LOCUS       DBNGSX010000001         5336 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1454513, whole genome shotgun sequence.
ACCESSION   DBNGSX010000001 DBNGSX010000000
VERSION     DBNGSX010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1454513"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            359..1798
                     /locus_tag="AC16CB_00005"
     CDS             359..1798
                     /locus_tag="AC16CB_00005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR3340873.1"
                     /translation="MEQMQRNDKLIEQSGSSKTVNRLKLALLAAFIIAAGITAYITFV
                     FVRDFVTSWELTSLPGITIRDEKPTSTPDASGVIQDVESPLQQFGGPTPQPWDGASRV
                     TLLVMGLDYRDWEAGEGAPRTDTMMLLTINPLDRTAGILSIPRDLWVNIPGFNYGRIN
                     TAYSLGEAYKYPEGGGPGLAMATVEELIGVPVDYYAQIDFGAFIRFIDEINGITITIE
                     KRMKVDQIGGKVKLLRPGTYTLNGELALGYARARNTEGGDFDRAFRQQEVIIAIRKRL
                     LESDNLPNLIAKSPVLYQELSSGVHTNLNLDQAIKLAWLSAQIPKENIKRGVIGPPDQ
                     VLLVQSPEGDQVLKPIADQIRLLRDTVFFHNRPESPAAVNLASIDRVTAEAPKISVLN
                     GTTTTGLAASTLEFLQSQGITAEAGNAEQVYNNTTIFNYTGKIYTVQYLVDLMKIDQN
                     HIFNRYDPTSQVDVVVILGMDWAANNPAP"
     gene            1871..4081
                     /gene="recD2"
                     /locus_tag="AC16CB_00010"
     CDS             1871..4081
                     /gene="recD2"
                     /locus_tag="AC16CB_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SF1B family DNA helicase RecD2"
                     /protein_id="HIR3340874.1"
                     /translation="MDTLTGSVERITYYNPENGYSVVRLRPERGKVPGLNRDGLVTVV
                     GNLPELAPGEYLRLSGRWTNHPKHGLQFQSEICEQAQPATLAGIRRYLGSGLIKGIGP
                     KLAERIVDSFGEQTLEVIEEHPERLYEVPDIGPKRSTLIASAWEEQKQVKEIMLFLHS
                     HEVSTNLAVKIYKQYGDQSLEVVQSDPYRLARDIFGVGFKTADRIAQALGLPADHPSR
                     VEAGLIYTLNEMSNEGHVYAPAPELSERAIQLLDVPAELVPPAMERLAKDDRIRQDVL
                     PPPDTTPKGETPALREPAGAYGLPAVYLTPLYHGEVGVAERLRALSQAQPRLNGIRLE
                     PWQNLASPLSPEQLSAVQAALKHPLSVLTGGPGTGKTTAVQALITALEAGRARLALAS
                     PTGRAAKRLSEATGRPASTIHRLLGYSPGEGFKHNAENPLPVDMLVVDEASMLDLILS
                     NQLLKALQPGTHLLLVGDVDQLPSVGAGDVLRDVIASGVAPVTRLSAIFRQAAGSHII
                     TNAHRINQGEMPLFPSDGDFFLFPGETAEEAGNWVQEVVCERIPKKFGYHPRDQVQVL
                     APLYRGPAGVHALNERLQEALNPPGAMKAEKRLFGQTFRLGDKVMQVQNNYDKDVFNG
                     DIGFVTAFDPIMHSMTVEFEGRKVDYDWSEADQLVLAYAISVHKAQGSEFTAVVLPIV
                     TAHYMMLQRNLLYTAVTRARQLCVLVGSRRAIGMAVKNDKVSRRYTALDWRLRL"
     gene            complement(4188..4481)
                     /locus_tag="AC16CB_00015"
     CDS             complement(4188..4481)
                     /locus_tag="AC16CB_00015"
                     /inference="COORDINATES: protein motif:HMM:NF020317.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HIR3340875.1"
                     /translation="MPIYITMMKLTDQGIKDIKNAPARFAEGVKGLEAVGGKMLGFYL
                     TMGEYDYVAITEGPSDEVALAYLLALGMQGNVRTNTIKAFPQELMAEMVKKLP"
     gene            complement(4775..>5336)
                     /locus_tag="AC16CB_00020"
     CDS             complement(4775..>5336)
                     /locus_tag="AC16CB_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HIR3340876.1"
                     /translation="SVHIFLRNRGKGEDFRYRGWREQAGGKWWWQSFFKVFLLQGVLL
                     WVISAPLLAAQISPIPAGLTWLDYAGLVLWGIGFAFEAVGDWQLRRFKADPANRGKLY
                     TGGVWRYTRHPNYFGDAALWWGFFMIAAAAGGWWSVFSPLLMTFLLVRVSGVAMLEKS
                     LAQAKPGYAEYIRTTSAFIPWFPKKN"
BASE COUNT         1182 a         1632 c         1452 g         1070 t
ORIGIN      
        1 gacaccatag cttctgtttt tctcttcccg tgaataccgt cattgcaaag gactccttcg
       61 gggcgactga agcattctta tcactcggac aggaagattg cttcgcctgc cagaatgcgg
      121 caggctcgca atgacgcggc attgcaatag ccatttcaag cgttctgacc aaccgatgag
      181 ggcgattata ccgtgttata atgcttccct gaggctgctg cgggtattcc tcgagcctat
      241 gcatcgtgta ctgtggcgca tgctaaaact cctggttatc tgcagggctt ttaaatctgt
      301 gaaggaaatg cccagatgat cttgaaggtc tctaaggcct tatggatctc agggttttat
      361 ggaacaaatg caaagaaatg acaaactgat tgaacaatct ggctcaagca aaactgtcaa
      421 ccggttgaag ctggcattgc tggcagcctt tattatcgcc gcaggcatca ccgcctatat
      481 cacatttgtc tttgtgcgcg atttcgtaac atcctgggag ctgacctccc tgcctgggat
      541 caccatccgc gatgaaaagc cgacttccac accagatgca tcgggcgtga tccaggatgt
      601 ggaatcaccc ttgcaacaat tcggaggccc cactcctcag ccttgggatg gcgccagccg
      661 cgtcacgctc ctggtgatgg ggctggatta ccgcgattgg gaggctggcg agggagcccc
      721 gcgaaccgac acgatgatgc tgctgactat caacccgctt gaccgcaccg ccggcattct
      781 ctcgatcccg cgcgacctgt gggtgaatat cccgggcttc aactacggtc gcatcaacac
      841 cgcctacagc ctgggtgaag cctacaaata cccagaagga ggcggtccgg gactggctat
      901 ggccaccgtg gaagagctga tcggcgtgcc ggttgattat tatgcccaaa tcgactttgg
      961 cgctttcatc cgcttcatcg acgagatcaa cggcatcacg atcactatcg aaaagaggat
     1021 gaaggtggat caaatcggtg gaaaggtcaa gttacttcgt ccaggcacgt atacgctgaa
     1081 tggcgaattg gccctgggtt atgcccgcgc ccgtaacacc gaaggtggcg atttcgaccg
     1141 cgccttccgc cagcaggaag tgatcattgc aatcagaaaa cggcttttag aatcggacaa
     1201 tttgcctaat ctgatcgcca agtcgccggt gctttaccag gagctctcct ctggcgtgca
     1261 taccaacctg aatctggacc aggctatcaa gctcgcctgg ctttcggcgc aaatacccaa
     1321 agagaacatc aagcgtggcg tcattggccc acccgaccag gtgctcctgg tgcaatctcc
     1381 agaaggcgac caggtattga agcccattgc tgaccaaatc cgcctgttgc gcgatacggt
     1441 gttcttccac aatcgtcctg agagcccggc ggcggtcaat cttgcatcga ttgatcgggt
     1501 aactgctgag gcacccaaga tctctgtgct caacggcacc accaccactg gattggctgc
     1561 aagcaccctg gaattcctgc aatcacaggg catcacagca gaggccggca acgccgagca
     1621 ggtttacaac aacaccacca tcttcaacta caccggtaaa atctacaccg tccaatacct
     1681 ggtcgacttg atgaagatcg accagaacca catcttcaat cgctatgatc caaccagcca
     1741 ggtggacgtg gtggtcatct tggggatgga ttgggctgca aataatccag cgccataatc
     1801 aagcgctgct tgtatcacgt gttccggtgt cacgtgataa tgaaacactt ggcaccggag
     1861 cacgtgacaa ttggacacat taaccggctc agtagagcgc attacctact ataacccgga
     1921 gaacggctat agcgtggtgc gcctgcgacc tgagcggggc aaggtgcccg gcctgaaccg
     1981 agatggcttg gtgaccgtgg tcggcaacct gcccgagctt gctccggggg agtatctgcg
     2041 gctgagtggt cgctggacca accatcccaa gcacggactt caatttcaaa gcgaaatttg
     2101 cgaacaagct cagcccgcca cgctggcggg catccgccgt tatttgggct ccggtctgat
     2161 caagggcatt ggcccgaagc tggcagagcg gatcgtcgac agcttcggcg agcagaccct
     2221 ggaggtgatc gaagagcacc cagagcgcct gtatgaagtg ccagatatcg gccctaagcg
     2281 ctcgacctta atcgccagcg cctgggaaga gcagaaacag gtcaaggaga tcatgctctt
     2341 cctgcacagt catgaggtga gcaccaacct ggcggtgaaa atctacaagc agtacggcga
     2401 ccagtcgctg gaggtggtgc aaagcgaccc ctaccggctg gcccgcgata ttttcggggt
     2461 ggggttcaaa accgccgacc gcatcgccca ggccctgggc ttgcccgccg accacccttc
     2521 gcgggtggag gcaggcctga tctacacctt gaacgagatg agcaatgaag ggcatgtcta
     2581 cgcccccgcc ccagagctat ccgagcgcgc catccagctg ctggatgtgc ccgccgagct
     2641 agtgccgcct gccatggagc gcctagccaa agacgaccgc atccgccagg atgtattgcc
     2701 ccctcccgat actacaccaa aaggggagac ccccgcattg cgcgaacccg cgggggctta
     2761 tggcctgccc gccgtttacc tgacaccctt gtatcacggc gaggttggcg tcgccgagcg
     2821 gctgcgcgcc ctgagccagg cgcagcctcg ccttaatgga atccggctcg agccctggca
     2881 aaacctggcc tcgcctctct cgccagagca gctttccgct gtgcaggctg ccttgaaaca
     2941 tcccctcagc gtgctcaccg gcggtccggg tacgggcaag accaccgccg tccaggcgct
     3001 catcactgcc ttggaggccg ggcgagcccg tctggcccta gcttcgccca ccgggcgcgc
     3061 cgccaaacgc ctctcggaag ccacaggacg cccagccagc accatccacc gcctgctggg
     3121 ctattctccg ggcgagggct tcaagcacaa cgccgagaac ccgctgccgg tcgatatgct
     3181 ggtggtggat gaggcctcca tgctcgacct gatcctctcc aaccagctgc tcaaggctct
     3241 tcagccaggc acgcacctgc tgttggtggg tgatgtggac cagttgccct ccgtgggcgc
     3301 cggcgatgta cttcgcgatg tgatcgccag cggggtcgca ccggtcaccc gcctgagcgc
     3361 catcttccgc caggccgccg gttcccacat tatcaccaac gcccaccgta tcaaccaggg
     3421 agaaatgccg ctatttccct cagatggcga ttttttcctc ttccctggtg agacagccga
     3481 ggaggccggc aattgggtgc aggaggttgt ctgcgaacgc atccccaaga aattcggcta
     3541 ccacccccgc gatcaggtgc aggtgctggc acccttatac cgcggcccgg caggcgtgca
     3601 cgctttgaat gagcgcctgc aggaagccct aaacccgccc ggtgcaatga aagccgagaa
     3661 gcgtctcttt gggcagacct tccgcctggg cgataaggtc atgcaggtgc agaacaacta
     3721 cgataaagat gtgttcaatg gggacatcgg ctttgtgacc gccttcgacc ccatcatgca
     3781 cagcatgacg gtggagttcg aaggccgcaa agtggattac gactggagcg aagccgacca
     3841 gctcgtgctg gcgtacgcca tctcggtgca caaggcgcag gggagcgagt tcaccgccgt
     3901 ggtgttaccc atcgtgacgg cgcactacat gatgctgcag cgcaacctgc tctatacggc
     3961 ggtcacgcgc gccaggcaat tgtgcgtgct ggtgggcagc cgccgcgcca tcggcatggc
     4021 agtgaagaac gacaaggtct cacggcgcta caccgccctc gactggcggc ttagactgta
     4081 aaactctctt ctactcggaa atgctctctg tatacctggg ggtggcgcac ccggtttact
     4141 gagctacgca tccaataaga tacctattaa gtccctgctg catcagctca cggcagcttc
     4201 tttaccatct ccgccatcaa ttcctgaggg aatgccttga tggtgtttgt gcgcacattc
     4261 ccttgcatgc ccagcgcaag caggtaggct aacgcgacct catcgctggg gccttccgta
     4321 atagcgacat agtcgtactc tcccatggtg aggtagaagc ctaacatctt cccgcccacc
     4381 gcttcaagac ccttaacccc ttctgcgaac cgtgcggggg cattcttgat atccttgatg
     4441 ccctggtctg tcaatttcat catagtgata tagattggca tagatcattc ctcctatcag
     4501 atcgtaaatt attaagcctg cgccaccccc acttcaaacc cacccagtgc aaggatcgaa
     4561 gccagggatt caattgcgat ggagtttcac actccgttga gccaattgaa tataaaaatg
     4621 ccactgacct atcgtcagcg gcacaaagga taaccacgtt gctgcccgct ttcagcgatc
     4681 actgggttgg gcaacgagaa gcattattat tatacaagac ggtacatcag agtcaatata
     4741 gtgaaatatt tcactaacaa ctcacccctt aagctcaatt cttcttagga aaccacggga
     4801 tgaaggcgct ggtggtgcgg atgtattccg cgtagcccgg cttggcctgg gccaggcttt
     4861 tctccagcat ggctacaccc gagacgcgca ccagcaaaaa agtcatcaaa agcgggctga
     4921 agacactcca ccagccgccg gctgccgcgg cgatcatgaa aaagccccac caaagggcgg
     4981 cgtcgccaaa ataattggga tggcgcgtgt agcgccacac cccgccggtg tagagcttgc
     5041 cccggttggc cgggtcagcc ttgaagcgcc ttaactgcca atcgcccacc gcttcaaatg
     5101 cgaatccaat cccccacaat accaggccgg cataatccag ccaggtcagc ccggctggta
     5161 tggggctgat ctgcgccgct agcagcgggg cggagatcac ccacagcagc acaccctgta
     5221 gcaggaacac cttgaagaag ctctgccacc accacttgcc gccagcctgc tcccgccagc
     5281 cgcggtagcg gaagtcctcg cctttgccgc ggttgcgcag gaagatgtgc acagac
//
LOCUS       DBNGSX010000002         4027 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1662383, whole genome shotgun sequence.
ACCESSION   DBNGSX010000002 DBNGSX010000000
VERSION     DBNGSX010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1662383"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1132)
                     /locus_tag="AC16CB_00025"
     CDS             complement(80..1132)
                     /locus_tag="AC16CB_00025"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HIR3340877.1"
                     /translation="MNNNHHWDQQGFHLVHSLEDDAWKAFVDNHPQGNIFHTPEMFEV
                     FKHTQGYRPSLWAVRGSDGQILALMLPVEITLSNVFRRLTSRNVVYGSILYEQSEKGK
                     EALEMLLKAYIRQTGGKPLFTELRNIFDLSPIQSLLQKCGFSYEDHLNYLINLDHPVD
                     AVFQNIGQRTRKNIRKAINRGEVQIEVVRDLQQMDDCYQLIWKTYRAASVPPPSKNMF
                     EAAYDVLGPKEMVRFTLARVNGVPVAVSADLLYKHVIYGWYGGMDRAYSAYSPNELLT
                     WHILKWGAEQGYHVYDFGGAGKPDEKYGVRDFKAKFGGQLVCYGRNTYVHNPITLSIS
                     KVGYQIYQGIVEARTS"
     gene            complement(1174..2160)
                     /locus_tag="AC16CB_00030"
     CDS             complement(1174..2160)
                     /locus_tag="AC16CB_00030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3340878.1"
                     /translation="MQNHRNNWLAISWAPYSRMSDTFAKELGGKLYCIHYLRFQSPPH
                     APLKYILQSFRTLQVLFKERPGAIHVQNPPFVSGLVVYLYCLISGAKFVLHYHSAAFA
                     KIWDWGLPIQKFLARRAVTNIVTNQHWADTVRSWGGHALVMTDPFLDLPEGEPFPVKT
                     GFKVAFVSTFAQDEPIEAVLEAAAQLPEIHFYITGDTRKKPASFFDKAPANITFTGFL
                     DPYRQYPSLLRAADTIMVLTTRDHTLQLGGCEAVATGKPLITSDWPYLREVFPKGTVY
                     VSDSAESIRDGILEMQKRYTDLAKEVIVLRQESRQEWDTRLGQLQELVAGAA"
     gene            complement(2197..3423)
                     /locus_tag="AC16CB_00035"
     CDS             complement(2197..3423)
                     /locus_tag="AC16CB_00035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3340879.1"
                     /translation="MNLIYLTGTYPILTTTFIDREIKMLRQWGVDLKIVAIRRPPADM
                     PLSKDQKELQQGVIYLLPVAWMSLIASHLYFAFLRPRRYFQSLIFLMTRSHPSLKARI
                     KTLLHFGEGVYAAYLVRKHQFREFHAHFVDRAATVALVAGRLLGKPYSLSIHAGADIY
                     VNPVLLREKITEARRAVTCSLYNKTHLEALIGQDLSHKISYIHHGLDLNEYQPSPPSS
                     NGKPLILSVGQLARRKGFAQLIKGCRGLKDQGYEFNCQIVGQGPQREELDELVSQLSL
                     EDTVTLLGAIPHDEVIERYRQATMFVLPCIQTDEGDVDGIPNVICEAMAMQLPVISTS
                     VSAIPEILEHEVNGLLLPPDDNQALVKAMAHLLDEPALRAQLGGNGRQSMIEIFDIER
                     NVRRFATTLWPEWFHQ"
     gene            complement(3450..>4027)
                     /locus_tag="AC16CB_00040"
     CDS             complement(3450..>4027)
                     /locus_tag="AC16CB_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340880.1"
                     /translation="VVGDLPSDAYAFRVDQKGVNLQNQNLYFYNNIWSDPSGTMGADL
                     SGDKNNFSGGEPVEVSNFILDNNLYWNGPNEIPPGKVASPRLDDARRVVTDPLLNTNH
                     AAIVLPRWNGSAFLSGSLTIQQEFERLVELYGKLPANSPAIGKADPAFAPPDDILGRP
                     RSASPEMGAYELDALASVPRVFLPLVTNNTP"
BASE COUNT          949 a          995 c         1048 g         1035 t
ORIGIN      
        1 gtgtaaccac agcaggtata aaatccatat atttaactct tttttataaa ggagtatttg
       61 ttgattagac gtctactagt taagatgtgc gggcttcaac gatgccctga taaatttgat
      121 accctacttt gctgatcgat agcgtaatgg ggttatgcac atacgtattg cgaccataac
      181 ataccagttg accgccaaat ttggctttga aatctcgtac accatatttc tcatcgggct
      241 tgccagcgcc gccgaagtcg tacacatggt agccttgttc tgcaccccat ttcaggatgt
      301 gccatgtcag cagttcgttc ggtgaatacg ccgagtatgc ccggtccatg ccaccatacc
      361 agccatagat cacatgttta tacagcaaat ccgccgatac agctacaggg accccattca
      421 cgcgtgccag ggtaaagcgc accatttcct ttggtccaag cacatcatag gcagcctcaa
      481 acatattttt gctgggagga gggacgctcg ccgcacgata ggttttccaa atgagctggt
      541 aacaatcatc catttgctgc aggtctcgca ccacttcgat ttgcacctct ccacggttga
      601 tcgctttgcg tatattcttg cgcgtccgtt gaccgatatt ttgaaagacg gcatccactg
      661 gatgatccag gttgatcagg taatttaggt gatcctcata agaaaagccg catttctgca
      721 agaggctttg gattgggctc aaatcgaaaa tgttgcgcag ctcggtgaat agcggtttcc
      781 caccagtctg gcggatgtat gctttcagga gcatttccaa tgcttctttg cctttttcgc
      841 tctgctcgta caagatgctc ccgtagacta catttcgact ggtgagtcgg cgaaacacat
      901 tgcttaaagt gatctcgacg ggtagcatca gtgccaggat ttgtccgtcg ctacccctaa
      961 cagcccatag cgatggacga taaccctgag tgtgtttaaa cacctcaaac atttctggtg
     1021 tgtggaagat attcccttgg ggatgattat cgacaaaggc tttccaagca tcgtcttcca
     1081 gactgtgtac taaatgaaat ccctgttgat cccaatgatg attattattc atgtctggta
     1141 tgtccagaag gaagataaga agagtaactc aggttacgct gcgccagcta ccaactcttg
     1201 tagttgcccc aggcgggtat cccattcctg ccggctttct tgacggagga cgatgacttc
     1261 tttggctaaa tccgtgtatc gcttttgcat ttccaatatt ccatctcgaa tgctttccgc
     1321 ggaatcggat acgtaaacag tacctttagg aaagacttct cgtagatagg gccagtcgga
     1381 agtaatcaat ggcttgcctg tcgccactgc ctcacagccg ccgagttgga gcgtatggtc
     1441 tcgtgtggtc agcaccataa tcgtatcggc tgccctgagc aagctgggat actggcggta
     1501 agggtctaaa aatccggtga aggtgatatt tgctggggct ttatcaaaaa agctggctgg
     1561 tttttttctg gtatcccccg taatataaaa gtggatctct ggtaattgtg ccgcggcttc
     1621 caaaacggct tcgatcggtt catcttgagc aaacgtgctg acgaaagcca ctttaaaccc
     1681 ggttttgacg gggaaaggct cgccctcggg taaatcgagg aagggatcgg tcatgaccag
     1741 ggcatgcccg ccccaggacc ggactgtgtc cgcccaatgc tggttcgtca cgatattggt
     1801 cactgcgcgc cgcgccagaa acttctggat aggcaatccc caatcccaaa tctttgcgaa
     1861 tgctgcacta tggtagtgaa gaacaaattt cgcgccgctt ataaggcaat agaggtacac
     1921 caccaagcca ctgacaaagg gagggttctg gacatggata gcaccagggc gttccttaaa
     1981 taaaacctga agagtgcgaa atgattgcaa gatgtacttc aaaggcgcgt gggggggtga
     2041 ttggaagcgt aaataatgga tgcagtacag ttttcccccc aactctttag caaacgtgtc
     2101 gctcatgcgg ctataaggag cccaggatat tgctagccag ttgtttcgat gattttgcat
     2161 ttctacctct tagtgaatta cagatgtgca aaaaaattat tgatggaacc attccggcca
     2221 caacgttgtg gcaaatcgcc gtacattccg ttcgatatca aaaatttcta tcatggattg
     2281 ccggccatta cctcccaatt gggcgcgcaa ggcgggctca tccagcaagt gggccattgc
     2341 tttcactagg gcttggttgt catctggggg caacagcaaa ccgttcactt catgctctaa
     2401 tatttcaggg attgcagaaa cgctggtgga gatcacgggt aactgcattg ccatggcttc
     2461 gcaaatcacg ttagggatgc cgtccacgtc cccctcatcg gtttgtatgc agggtaatac
     2521 aaacattgtc gcctgtctgt acctttcaat gacttcatcg tgagggatgg caccgagcaa
     2581 ggtcaccgta tcttctaacg acaattggct tactaactcg tctaactcct cccgctgagg
     2641 cccctgcccg acgatttgac agttgaattc atacccctga tctttcagcc cacggcaccc
     2701 tttgatgagc tgcgcgaaac ctttccttcg cgccagctgc ccaaccgaga ggatcagcgg
     2761 cttgccattc gaggaaggcg gggacggctg gtattcgttc aagtccaatc catgatggat
     2821 atagctgatt ttgtggctaa ggtcttgacc gataagtgcc tccaggtgcg tcttgttgta
     2881 cagcgagcag gtgacggcgc ggcgggcttc cgtgattttt tcacgtagca ggacaggatt
     2941 gacatagatg tccgctccag catgtatcga taagctgtaa ggtttaccca gcaatctgcc
     3001 tgccaccaat gcgacagttg cagctcgatc aacaaaatgg gcgtggaatt ctcgaaattg
     3061 gtgcttgcgc acgagatagg ctgcataaac accttcgcca aaatgcagta atgtcttgat
     3121 gcgtgctttc aggctagggt gagatcgagt cattagaaaa atcaggcttt ggaagtagcg
     3181 ccgtgggcgc aaaaaggcaa agtacaggtg gctggcgatt aaactcatcc aggctaccgg
     3241 gagcaggtag attacgcctt gctggagctc tttctgatct ttggagagag gcatatccgc
     3301 cggtgggcgg cggatggcta caattttcag atctacgccc cactgacgca gcatcttgat
     3361 ttcccggtca atgaatgtcg tggtcaggat cgggtatgtg ccggtgaggt aaatcaggtt
     3421 catttatctt aggcttgtta ctttcgctct caaggggtgt tattggtgac aagaggcaaa
     3481 aatacgcgcg gaacagaggc cagcgcatcc agctcgtaag cacccatctc gggagaggca
     3541 ctgcgcggtc ttcccaagat gtcatcgggt ggtgcaaagg ctgggtctgc ctttccgatg
     3601 gctgggctgt tagccgggag cttgccgtaa agctcgacca ggcgctcgaa ctcctgctgg
     3661 atggtcaggc tgccgctcag gaaggccgag ccgttccaac gtggcaaaac gatggctgca
     3721 tggttggtgt tgagcaatgg gtctgtcaca acacgccggg cgtcgtccaa tcgggggctg
     3781 gctactttcc cgggcgggat ttcattaggt ccattccagt acaggttgtt gtctaggatg
     3841 aaattgctca cctccaccgg ctcgccgccc gagaagttgt tcttatcccc agacagatca
     3901 gcacccatcg tgccggacgg gtctgaccag atgttgttgt agaagtagag gttctggttc
     3961 tgcaggttta cgcctttttg gtcgacacgg aaggcatagg catccgaagg caggtcaccg
     4021 acgacgg
//
LOCUS       DBNGSX010000003        11560 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1593110, whole genome shotgun sequence.
ACCESSION   DBNGSX010000003 DBNGSX010000000
VERSION     DBNGSX010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11560)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11560)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11560
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1593110"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="AC16CB_00045"
     CDS             complement(<1..1020)
                     /locus_tag="AC16CB_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007196187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR3340881.1"
                     /translation="MKTFLSILLALTLLLTACAQATPTEAPPEATELPAVEATEAPPQ
                     PPSAEGYAPPEGAMPPVPLAEGSQSTTPVAYKAGDPIKIAYMPPATEFNYYMAIGEGI
                     KALAAEAGVETFMLAPQSGSDIATQMKMLQDVTTQDVDAIILSTHDENAAAPLVKQAV
                     DMGIAVVIVNSDIASFPSPVNAVVGYSQRGGTYDLGKYAAEMVGGTAKVGVLEGLPGY
                     HSTERIGGFLDAFKEYPGMEIIASLPTAWNVETGNTAMMDLLQAHPEVNLVVCANDYI
                     AIGAATAAEALGRSDVLIFGNDGDTAGLEDIYNGKWTATVNTTPFVMGKVALQVALDV
                     LNGTYP"
     gene            complement(1115..2101)
                     /locus_tag="AC16CB_00050"
     CDS             complement(1115..2101)
                     /locus_tag="AC16CB_00050"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3340882.1"
                     /translation="MERSTTELRKAPTWLAQLVAFRELSLILVLLIFGIIMSLVSPVF
                     LTWPNLEAILLSLSVEATIAVGMVILLISGGLDLSVGSTLGLTGVVAGLVLTAGLPAP
                     VAILSGLLAALGVGLVNGLLVSKMKINPFITTLGMMITIRGLLLVLAQGRAVLNLAQS
                     FTVIGQGRLFSVQYPIFVMLALVLAGDLLMRNARFFRQSYYIGGNEKAARLSGINVDL
                     VKIINYCIVALLAGVAGLLITARFGSASVTVGSGVELRVITATIIGGASLNGGEGSVF
                     GAFLGALFMAVLANALNLMGVDVYWQNLVTGLILIIAVVVDVINERRKNLQR"
     gene            complement(2149..3681)
                     /locus_tag="AC16CB_00055"
     CDS             complement(2149..3681)
                     /locus_tag="AC16CB_00055"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR3340883.1"
                     /translation="MEDVLRAEHICKRFPGVLAVDHVSLSLRRGEILALVGENGAGKS
                     TLMQILGGALRPDEGQILLEDRPLVFNSPEDAIRSGISMVFQELSLVGSLSVAENIFV
                     NRQPVGALNVIRWGELYRQTADFLDRFELDLNPRWLVKQLSMGQQQILEILKAISTNP
                     KVLILDEPTSSLTESEIAYLFQNIRRLQAQGMSFIYITHKLSEVFQIADRVMVMRDGK
                     YVDSKPIRDVTENNLVAMMVGRQITHLYGARTDKDRDEYFRVEGFSRRGSFQDVSFSL
                     RRGEILGFAGLVGAKRTEVGRAIFGIDRRDSGRIILNGNELNIRKPEDAIRYRIAYLT
                     EDRKGQGLFLSMSLRDNLVAPSFNKFTSRLGFVRHPLLERYAGEMVRQFDIVTPSMEK
                     KVLNLSGGNQQKTLIAMWVGIQPEVIIFDEPTRGVDVGARAEIYARLRDFAATGVGVI
                     MISSDLPELIGMCDRILVMHQGRITGEVGREDFSEALILSFAAGLEQVVRSMDNRNAQ
                     AQ"
     gene            complement(3761..4789)
                     /locus_tag="AC16CB_00060"
     CDS             complement(3761..4789)
                     /locus_tag="AC16CB_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR3340884.1"
                     /translation="MKNKMYILIGFLVAISMLAAACAQQPAAPASEAQPESAQPATAD
                     EEYIYVSAMGNLEFFNAHKYGWKWAGERLGVTATYVGPAEYDMNAMVAAFDQAIAKKP
                     KGICTFAVEPVLTPSINKATEAGIPVVTILGDLPESKRIAFVGSRQYDLGFTGGVKIA
                     EALGGKGKVAILSIPGVGMFDDRETGFRAAFEAYPDIEVVQVGDTKADTVTAVSVAKD
                     IMQRYPDLAAFVGTDSTGGIGAATAVEEAGKVGQVKTVAMDRNSDVLEKIQKGTLTGT
                     VAQDDAAMAFWCLQTLYNYVHNQAPLTTDNAKAGASTGPTNVFMSVNYIDASNLQYFL
                     DANALYTK"
     gene            complement(4866..6023)
                     /locus_tag="AC16CB_00065"
     CDS             complement(4866..6023)
                     /locus_tag="AC16CB_00065"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HIR3340885.1"
                     /translation="MSDQKVQYNIIQDDLSGFADKGPAFVTIGECMVRDTPTDMQRLE
                     MTRQVHISLAGSEYTLAMTLARLGIPSAYITRLPDNPYGWMVRDTARSQGIHTDYIVW
                     APKAEPIGRLLYELGRTPRKSVAYYQRMCSAASRLSPGMVDWGSALRDCKLFHTSGIT
                     FGLANHSKYEHNYLLDTFHEAMANRQPDTLVGLDFNYRSTLWKPEQCKAVMTPLISDY
                     IDILITTIEDMAALYGIGCGQYSAEQIDKGDIGELTDSDIKAFAEQVGELFKTRIVAI
                     TIRYPDTFEQHRWEAAAMDAEGHFFRSPAVKPMVLLDRLGGGDTWNGGFYYGLLTTGF
                     NPDGIAKGVLVGDAATRIKQTLMFDLPIVTRHEVQDLMKADVLGGGKRVAR"
     gene            complement(6096..6866)
                     /locus_tag="AC16CB_00070"
     CDS             complement(6096..6866)
                     /locus_tag="AC16CB_00070"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3340886.1"
                     /translation="MKLKDRVAIVTGGAMGIGWGICKVLSQEGAKIVVVDWDEKVGKK
                     TAEELNQGGSEAIFVLCDVSNEDQVKAMIEATLKRFGKIDILVNNAGIGVYKPVLETS
                     SADWDRCLAVNLKGQFLCSKYAIPHMQEIGKGAIVNISSVHSFQTVNGVAPYAASKGG
                     ITALTRNMAIDYGPAIRVNSIAPGWVLTPLIQSIFDSYPDPADQQRQVERRQVMKRIG
                     RPEDIGYAVAFLASDEASFITGTQLYVDGGLTAQLETW"
     gene            complement(6897..8075)
                     /locus_tag="AC16CB_00075"
     CDS             complement(6897..8075)
                     /locus_tag="AC16CB_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3340887.1"
                     /translation="MSDSDRYRIERLQKKMADAGLAALVCRLSENVVYLTDYWPHHGF
                     SVAVLPQQGKPWLFVPEVELEYTHPEWADVTTFGWGLLKDGDLYENYRRLLTLAVEKL
                     GLKGKLVGVEQTFEVTGPTYRSAEPVTPAKPWADLLDTVFAGSTLVDNNALLSEVRAT
                     KSEYELKKLRVANEIAEMGIHHALSELKPGMTEAQVGALIEYKIRADGPGHKGARLVR
                     AMAEVAAGPVGSTKGTLLIPSTTYIIQAGDFVMIELATVVDGYWSDLTYMAVAGDPNE
                     RQKEVYNALLEAQQAAVQQMRAGASYTDPDTAARKVLQAAGLGDYFVHITGHGVGYRY
                     HEFIPFLMPGITGTLEQGMVSSVEPGVYIPGFGGLRIEDNVAVGVEGPVLLSSPRKPW
                     "
     gene            complement(8147..8827)
                     /locus_tag="AC16CB_00080"
     CDS             complement(8147..8827)
                     /locus_tag="AC16CB_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008204170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HIR3340888.1"
                     /translation="MRKIILERQTSPDPEILQGFKDLLKVYSPSCVVADSQERQGVMR
                     SYMRPISMETRFVGPALTVRLEPGNQVDCLDALSVAQVGDVIVVDAAGETETSIWGGL
                     MSGLCMMKGVVGAVVDGAIRDTDETRDLGFFIFSKAIVPRSTHSPYSGRMEPIEVNVP
                     IQCAGVVVRPGDLVLGDEVGVVVIPHENAADVLLKAREQADKEEKTRAKIREGKTVEQ
                     LLAEFGRL"
     gene            complement(8830..9654)
                     /locus_tag="AC16CB_00085"
     CDS             complement(8830..9654)
                     /locus_tag="AC16CB_00085"
                     /inference="COORDINATES: protein motif:HMM:NF013757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR3340889.1"
                     /translation="MSRPVKTVNQDEARYNIRVLDRAISILSLLADGKPRTSTEISEA
                     IELSPSTTFRMLSTLAYYHYVLREETTNQYRLGLACLELAHAYLTGNDLRRISLLELE
                     TLRNETKETVHLAVLEDMEIVYLEKLPGLHAIGLMSSRVGGRSPAYCTGVGKVLLAYQ
                     KLDRVQAYYQAYGLPRYTENTITDLRTLMSVLEAIRRVGYGFDHGEHEDEVRCVAAPI
                     FDFTGQAIAALSISGPEARMDPIEENNDLISLILHASMNISRQLGYNLFKNSSEGR"
     gene            complement(9651..9938)
                     /locus_tag="AC16CB_00090"
     CDS             complement(9651..9938)
                     /locus_tag="AC16CB_00090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HIR3340890.1"
                     /translation="MIERHVTFEVIPDKTQDFENLFVKAYRPAMASMPGFIKVELLRL
                     QDSSTIYQMIIRFETAEAASAWRNSPEHQGLSPQLKALYIASHLQVFEVIA"
     gene            complement(9996..10643)
                     /locus_tag="AC16CB_00095"
     CDS             complement(9996..10643)
                     /locus_tag="AC16CB_00095"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - (R,S)-4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HIR3340891.1"
                     /translation="MDQNAALGMIVESGVVAIMRAKNSDQLLDAARAVLAGGVQAIEV
                     TMTTPGALDVIHQATSQFGSEVLFGVGSVLDPETARAAILAGAKFVVCPTLNLKTIEI
                     CKRYSIPVMPGAYTPTEILTAWEAGAEIIKVFPASAGGPPYIKAVKAPLPHIRLAAVG
                     GVDLTNTADFIKAGVEVVGVGGELVNQKLLDARDFAAITERARGFRQGVQAGRGK"
     gene            complement(11078..11488)
                     /locus_tag="AC16CB_00100"
     CDS             complement(11078..11488)
                     /locus_tag="AC16CB_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR3340892.1"
                     /translation="MEIPRHWRLRKQRYSMVGEVCPHCEAKLFPPRDVCPECGQEAKT
                     LYQFSGRGEVYSYTTVYDPPAGFEEAAPYTVALIKLEEGPLVTAQLTDMEGQQVEIGT
                     PVEMVTRKLRNDGDERGMVVYGYKFRPLMSQAPA"
BASE COUNT         2527 a         3196 c         3178 g         2659 t
ORIGIN      
        1 ggggtaggtg ccattgagca cgtcgagggc gacctggaga gccaccttgc ccataacgaa
       61 gggcgtggtg ttgactgtag cagtccactt tccattgtag atgtcttcca gcccagcggt
      121 atcgccgtcg ttgccgaaga tgagcacgtc gctgcgcccc aaggcttcag ccgctgtggc
      181 agcaccgatg gcgatgtagt cgttagcaca cacgaccagg ttgacttcgg gatgagcctg
      241 gagcaggtcc atcatggcgg tgttgccggt ctcgacgttc caggcggtgg gcaggctggc
      301 gatgatctcc atgccggggt actctttgaa ggcatccagg aagccgccga tgcgctcggt
      361 ggaatggtag ccgggcaggc cttcgagcac accgaccttg gcggtgccgc cgaccatctc
      421 agcagcatat tttccaaggt cataggtacc cccccgctgg ctgtagccga cgacggcatt
      481 gacggggctt gggaaggaag cgatgtcaga gttgacgatc accacggcaa tgcccatatc
      541 cacagcctgc ttgaccagag gggcggcggc gttctcgtcg tgggtggaga ggatgatggc
      601 atccacgtcc tgggtggtga cgtcctggag catcttcatc tgggtagcga tgtcggagcc
      661 agactgtggg gcaagcatga aggtttccac gcctgcttcg gctgccagcg ccttgatgcc
      721 ctcgccgatc gccatgtagt agttgaactc ggtggcgggg ggcatgtagg cgatcttgat
      781 cgggtcgcca gctttgtagg ctactggagt ggtggattgc gatccctctg ccagcggcac
      841 aggcggcatg gctccctccg gtggggcgta gccttcagcc gatggtggct gaggtggtgc
      901 ctcggtcgct tccactgcgg gcagttcggt cgcttcaggc ggggcttcgg ttggtgtcgc
      961 ctgtgcgcag gcagtcaaca gcagggttag cgctagaagt atggatagaa aggttttcat
     1021 ctcaggtact cctccatttt tttgtgtgag tctgctttta ttaggaatct acttgaatta
     1081 gcataccggg tcattttttc tggggacctc ctgttcatct ctgaagattt tttcgacgct
     1141 cgttaatgac atcaacaacc actgcgataa tcaaaatcaa gcctgtaacc aggttttgcc
     1201 agtagacgtc tacccccatt aaattcaatg cgttggcaag caccgccatg aacaaagcgc
     1261 ccaaaaatgc gccgaacacc gacccctctc cgccgttaag gctagccccg ccaataatcg
     1321 ttgcagtgat gacgcgcaac tctacaccac tcccaactgt gacggatgcc gagccaaagc
     1381 gggcggtaat cagcaagcca gcgacgcccg ctaataatgc cacaatacag taattgatga
     1441 tcttgaccaa atcaacgttg atgcccgaaa ggcgggcagc tttttcattt ccgccaatgt
     1501 aataggactg gcggaagaaa cgcgcattgc gcatcagcag atctcctgcg agcactaacg
     1561 ccagcatgac aaagatgggg tactgcacgc tgaacaatcg tccttgaccg atcacggtaa
     1621 aagactgcgc caggtttagt actgccctcc cctgagccag taccaataac aagccgcgaa
     1681 tggtgatcat cattcccaag gtcgtaataa aagggttgat cttcattttt gaaaccagaa
     1741 gcccgttcac caggccaact cccaaggcag ccaacaatcc ggagaggatc gccaccgggg
     1801 ctggcaagcc agcagtcagg acaagcccag cgaccacacc tgtaagccct aatgttgagc
     1861 caacagacag atctaaaccg cctgagatca acagaatcac cataccaact gcgattgtcg
     1921 cctctacaga aaggctgagc aggatcgctt caaggttagg ccaagtcaga aaaacaggcg
     1981 aaaccaagct catgatgatg ccaaagatca gcagcaccag aatcaggctt aattcacgga
     2041 aagcgacgag ctgcgctagc caagtagggg cttttctgag ttcggttgtg cttctttcca
     2101 ttaacacctg ctctatgcgg gtttagttgg ataaaaatat ataactcgtc attgcgcctg
     2161 ggcatttcgg ttgtccatag atcgaacaac ttgctccaat ccagcggcaa aagaaaggat
     2221 caatgcttca gaaaaatctt cacgccccac ctcacctgtg atgcgcccct gatgcattac
     2281 caggatgcgg tcgcacattc caattagctc gggcaaatcc gaagagatca taatcacgcc
     2341 gacgcctgtt gcagcgaaat cgcgcaacct ggcatagatc tcagcccgcg cgccgacatc
     2401 cacgccgcgg gtgggctcgt cgaaaatgat tacttcgggc tggatgccca cccacatggc
     2461 tatcagtgtt ttctgctggt tcccgcccga caggttgagc accttctttt ccatagaagg
     2521 cgttacaatg tcaaattgcc tgaccatctc tccggcgtaa cgctcgagga gcgggtgtct
     2581 gacgaaacct aagcgacttg tgaacttatt gaacgagggg gcaaccaggt tatcgcgtag
     2641 tgacatactc agaaagagcc cctggccctt tcgatcttcg gtaaggtagg cgattctgta
     2701 tcgtatagca tcttccggtt tgcggatatt cagctcgtta ccattcaata tgatccgccc
     2761 gctatctcta cggtcgatac cgaagatcgc ccgtcccact tcagtacgct ttgcaccaac
     2821 caaaccagcg aagccgagga tctctccacg cctcaagctg aagctgacat cctggaatga
     2881 gccccggcga gagaagccct ccacgcgaaa atattcatct cggtctttgt ccgtgcgagc
     2941 gccatataag tgggtaatct gccggcctac catcatggca actaggttat tctcggtaac
     3001 atctctgatt ggcttgctgt cgacatactt gccatcccgc atgaccatca cccggtccgc
     3061 aatctggaat acttcggaga gtttgtgggt tatgtaaatg aaagacattc cctgcgcctg
     3121 caggcggcgg atattctgga acagataagc aatctcgctc tcagtgaggg acgaggtagg
     3181 ctcatcgagg atgagcacct tggggtttgt tgagatggcc ttaaggatct ccaggatctg
     3241 ctgctgaccc atggaaagtt gcttgaccag ccagcgaggg ttcaagtcca gctcgaaccg
     3301 gtccagaaag tccgcggttt gacggtacag ctcaccccat cggataacgt tcagcgcgcc
     3361 aaccggctgc cggtttacaa agatattctc ggctaccgag aggctgccca ccaacgacag
     3421 ctcctggaaa accatgctga tgccagatcg gatggcatct tcgggtgagt tgaacaccag
     3481 cggacggtct tccagtaaga tctggccctc atccggacgc agggcgccgc ccaggatctg
     3541 catgagggtg ctcttcccgg cgccgttctc acccaccagc gccaggattt cgccccgcct
     3601 caggctcagg ctgacgtggt ccaccgccag cactcctggg aagcgcttgc agatgtgttc
     3661 tgcccgtaaa acgtcttcca tcggatcctt agaggggcac agccagtgtt gggacagcgc
     3721 aggacgcgaa gcactcgccg tgcccctatt tgagggaggg ttatttcgta tagagcgcgt
     3781 tggcgtctaa gaaatactgc aggttgctgg cgtcgatata gttgaccgac atgaacacgt
     3841 tggtcgggcc agttgaagcg cccgctttgg cgttatcagt agttagcggc gcctggttgt
     3901 ggacatagtt gtacagggtt tgcagacacc agaaggccat ggctgcgtcg tcctgagcca
     3961 cggtaccggt cagggtgcct ttctggatct tctccagcac gtcggagttg cgatccatgg
     4021 caacggtctt cacctgaccc accttgcctg cctcttcgac cgcagtagca gcaccgatcc
     4081 cgccggtgga gtccgtgccg acgaaggctg ccaggtcagg atagcgctgc atgatatctt
     4141 ttgccacact gacggcggtc accgtgtcgg ccttggtgtc gccaacctga acgacctcga
     4201 tgtcggggta ggcttcgaag gcagcacgaa aaccggtttc gcgatcatcg aacatcccca
     4261 caccagggat gctaaggatg gccactttgc ccttgccccc cagagcttcg gcgatcttaa
     4321 cgccaccggt gaatcccagg tcgtactggc gcgagcccac gaaggctata cgcttggatt
     4381 cgggtaggtc acccaggatg gtcaccaccg ggatgcctgc ctcagtggcc ttgttgatcg
     4441 aaggggtgag gacgggctcg acggcgaagg tacagatgcc cttgggtttc ttggcgatgg
     4501 cctgatcgaa ggctgccacc atggcgttca tatcatattc agccgggcct acatatgtgg
     4561 ccgtgacgcc caggcgctct ccagcccatt tccagccgta tttgtgggcg ttgaagaact
     4621 ccaggttgcc catggcagag acatagatat actcttcgtc cgctgtggct ggctgagcgc
     4681 tctcgggctg ggcttcgctg gccggggcag ctggctgctg tgcgcaggct gctgcgagca
     4741 tagaaatagc gaccagaaag ccaatcagga tatacatttt gtttttcatg cgaccttctc
     4801 cttcttacgt tagacttttt cttagggcaa gaatgccgat gcaccccacc cttttattgc
     4861 ctagctcagc gagcgacccg cttgccgccg ccgagcacat cagccttcat aagatcctgc
     4921 acttcgtggc gggtgacgat gggtaagtcg aacatcaaag tttgcttaat acgtgtggca
     4981 gcgtctccga ccaacacgcc ttttgcgata ccatcagggt tgaaaccggt agtcagcaaa
     5041 ccatagtaaa atccgccgtt ccaggtatca ccgccgccca aacggtccag cagtaccata
     5101 ggcttgactg ccggcgagcg gaagaaatgc ccctcagcgt ccatggcagc cgcctcccaa
     5161 cggtgctgtt caaaggtgtc tggatagcgg atggtgatgg caactatgcg ggtcttgaac
     5221 agctcgccta cctgctcggc gaaggctttg atgtcggagt cggtcaactc gccgatatct
     5281 cctttatcaa tttgctcggc gctgtactgc ccgcagccaa taccgtacag agcggccata
     5341 tcctcgattg tcgtgatcag gatgtcaatg taatcggaga taagaggcgt catgacggct
     5401 ttgcattgct caggcttcca caacgtggag cgatagttaa aatccagacc caccagggta
     5461 tcaggttgcc gatttgccat cgcctcgtgg aaggtatcca gtaggtagtt gtgctcgtat
     5521 ttgctatggt ttgccagtcc gaaggtgatg ccggaggtgt ggaacagctt gcagtctcgc
     5581 agggcagatc cccagtccac catacctggg gatagacgac tggcggctga acacatgcgc
     5641 tgataatagg ctacactctt gcgaggggtg cggcctagct catagagcag acgaccaatt
     5701 ggctcagcct tgggggccca aacaatgtag tcggtgtgga tgccctggct tcgtgccgtg
     5761 tcacgtacca tccaaccgta gggattgtct ggcaggcgag tgatataagc ggatgggatg
     5821 cccaggcgag caagggtcat cgccagagta tattcgctgc ctgccagtga gatgtgtacc
     5881 tggcgggtca tctccaggcg ctgcatatct gtgggtgtat ctcgtaccat gcattcgcca
     5941 atggtaacaa atgctggccc tttatcggcg aagccgctca ggtcgtcctg tatgatgtta
     6001 tattggacct tctgatcgct cataaatgag cttctcctta attaatctgt acttcagcta
     6061 tgacgcactc cggaagcgca tcgcaccttt tctgattacc aggtttctaa ctgagctgtc
     6121 aggccgccat ccacgtatag ttgggtaccg gtgataaagg aagcttcgtc gctagccagg
     6181 aaagctacgg cataaccgat atcctccggg cgaccgatgc gtttcatcac ttggcgccgc
     6241 tcaacctggc gctgctggtc agctgggtct ggatagctgt caaagatcga ttggatcaga
     6301 ggggtgagca cccagccagg tgcgatggag ttgacccgga tagccggtcc gtagtcgata
     6361 gccatgttgc gcgttagggc agtgattcca cctttggagg cagcataggg agcaacgcca
     6421 tttaccgtct ggaaagaatg caccgatgag atgttgacaa tggctccttt gccgatttcc
     6481 tgcatgtgcg ggatggcata tttggagcaa aggaactggc ctttgaggtt taccgccagg
     6541 caacgatccc agtcggcgct gctggtctct agaaccggct tgtagacacc gatgccagcg
     6601 ttattaacca ggatatcgat cttaccaaag cgtttcagcg tggcttcgat catagctttg
     6661 acttgatcct cgttggatac atcgcacagg acgaatatcg cctcgctgcc accctgattg
     6721 agctcttcgg cggtcttttt gccaaccttc tcgtcccagt cgaccacgac aattttcgcc
     6781 ccctcctgac tgagtacctt gcagataccc cagccaatgc ccatggcccc tccggtgaca
     6841 attgcgacac gatcttttaa tttcatggtt gacctccaat tttgtcctaa attaccttac
     6901 catggctttc gtggcgaaga gagcagcacc gggccttcga cgccgaccgc tacgttatct
     6961 tcgatgcgca aaccaccgaa gccagggata tatacacccg gttcgacgct gctgaccatg
     7021 ccctgctcca gtgtgccggt aatgcctggc atcaagaagg gaatgaactc atggtaacgg
     7081 tagcccaccc catgcccggt gatgtggaca aagtaatcgc caaggccggc agcctgcaat
     7141 accttacggg ccgcagtgtc cggatcggta tacgaggcac cagcgcgcat ttgctgtaca
     7201 gccgcttgct gcgcctcaag caaggcgttg taaacctctt tctgcctttc gttggggtca
     7261 ccagccacag ccatataggt caggtccgac cagtagccgt ctacaacggt cgccagttcg
     7321 atcataacga aatcgccagc ctggattatg taggtggtgg aagggatcaa gagtgttcct
     7381 ttggtgctgc ccaccggtcc ggcggctact tctgccatgg ctcgtaccag acgggcgcct
     7441 ttgtggccag gtccatcggc gcggatcttg tactcgatca gcgctcccac ctgggcttcg
     7501 gtcatgccgg gcttcagctc tgacagggcg tggtgaatgc ccatctcagc gatctcattg
     7561 gcaacacgca gcttctttag ctcatattcg gatttcgtgg cgcgcacctc ggacaggaga
     7621 gcgttattgt ctaccaaggt tgaaccagca aagacggtgt ctagcaggtc agcccagggt
     7681 ttggcagggg taactggttc ggcagaacga taggtaggtc cagtcacttc gaaggtttgc
     7741 tccacgccga caagcttgcc cttcaacccc agcttttcga cggccaaggt cagcagacgg
     7801 cgataatttt cgtacagatc gccatcctta agcaagcccc agccaaaagt tgtcacatca
     7861 gcccattcgg gatgcgtata ttctagctcg acctctggga cgaacaacca gggtttgcct
     7921 tgttgcggta ggacagccac cgagaatccg tggtgaggcc agtaatcggt caggtacacc
     7981 acgttttccg acaggcggca aaccagcgcc gccaggccag catctgccat tttcttttgc
     8041 aagcgttcga tacgatatcg atctgaatca ctcatgcgat cctcctagaa ctatttatcg
     8101 atcagccttg caaaagcgat tagctgcctt ttgcaaggct tgttaattaa agccgaccaa
     8161 actctgccag caactgttct acggttttgc cctcgcggat cttggcgcgg gtcttttctt
     8221 ctttgtcggc ctgctcgcga gcttttagaa gcacgtcggc ggcgttctcg tgtgggatga
     8281 ctactacgcc gacctcatcg cccagcacca ggtcgccggg gcggacgacc acaccggcgc
     8341 attgaatcgg aacgttgact tctatcggtt ccatgcggcc cgaataggga gagtgtgtgg
     8401 agcgcggcac gatggccttg gaaaagatga aaaagcccag atcgcgggtc tcatcagtgt
     8461 cacggatcgc gccgtccacc accgcgccaa ccaccccttt catcatgcac aaccccgaca
     8521 tgaggccgcc ccaaatggat gtctctgtct cgccggctgc atcgaccacg atcacatccc
     8581 caacctgagc aactgaaagg gcatcgaggc agtctacctg attaccgggc tccaggcgga
     8641 cggtcagcgc tggcccgaca aagcgtgtct ccatgctgat cgggcgcata tacgagcgca
     8701 tcaccccctg gcgctcctgt gagtcggcca ccacgcaaga agggctgtac actttgagta
     8761 aatctttgaa accttgtaaa atctcggggt ctggggaggt ttggcgttcc aggatgattt
     8821 tgcgcatgat taccttcctt ctgaagaatt tttaaacaag ttgtagccga gctgccgtga
     8881 gatattcatg gaggcgtgca gaatcaggct gatcagatcg ttgttctctt cgattggatc
     8941 catgcgggct tccgggccag agatactcaa ggcggcgatg gcttgtccgg tgaaatcgaa
     9001 gatcggcgct gccacgcagc gcacttcatc ttcatgctca ccatggtcaa agccatagcc
     9061 tacccggcga attgcctcca gcaccgacat caatgtgcgt agatcggtga tggtattctc
     9121 ggtatagcgc ggcaagccgt aggcctggta gtacgcctgc actctatcga gcttctgata
     9181 agccagcaat accttgccaa cgccggtgca gtaggcggga gaacgccctc ctacccggga
     9241 cgacatcaga ccgatggcgt gcaatccggg cagcttttca agatagacga tctccatgtc
     9301 ctccaggacc gccaggtgga cagtctcttt ggtctcgtta cgcaaggttt ccagctctag
     9361 caaggaaatc cgccgcaggt cattgccggt caggtaggca tgggctaatt ccaggcaggc
     9421 aagcccgagg cgatactggt tggtggtctc ctcacgcagg acatagtggt agtaagccag
     9481 agtggagagc atgcggaagg tggtgctggg gctgagttca atcgcctcgc tgatttcagt
     9541 cgatgtgcgc ggtttaccgt ccgcaagtag ggagaggatg ctgatcgccc ggtccagcac
     9601 acggatgttg taacgcgcct catcctggtt tacggttttc acgggacggc tcatgcaatc
     9661 acctcgaaca cctgcagatg gctggcgatg tagagcgcct tgagttgggg ggaaagcccc
     9721 tggtgttctg gggagttgcg ccaagcagat gccgcttcag ccgtttcaaa gcggatgatc
     9781 atctggtaga ttgtcgagct gtcctgcagg cgcaacagct ccactttgat gaagccgggc
     9841 atgcttgcca tggcagggcg gtaggctttg acgaaaaggt tctcgaaatc ctgggtttta
     9901 tcagggatta cttcgaaagt gacgtgccgt tcgatcatgg ctgcctccag aaatcagaca
     9961 gggttgagtt ggcctgtcct ttcgaacctg tctgattact ttcctcgccc tgcctggacg
    10021 ccctggcgga aaccgcgcgc ccgctcggta atcgcggcga agtcgcgcgc atccaacaac
    10081 ttttggttga ccaactcccc accaactccc accacttcaa cccccgcctt gataaagtct
    10141 gccgtattgg tgagatcaac cccgccgacg gcagccaagc ggatgtgggg caaaggcgct
    10201 ttcacagctt tgatgtaggg ggggccaccg gcgctggcgg gaaagacctt aatgatttct
    10261 gccccagcct cccaggcagt cagaatctca gtgggcgtat aggcgccggg cattaccggg
    10321 atggaatagc gtttgcaaat ttcgatagtc ttaagattta aggttgggca gaccacgaat
    10381 tttgcccctg ccaggatagc ggcacgggct gtttcggggt ccagaaccga gccaacgccg
    10441 aacagtacct cactgccaaa ttgactggtc gcctggtgga tgacatccag cgccccgggt
    10501 gttgtcatgg tcacttcgat ggcctgcacg cctccggcga gcacagctct ggcagcgtct
    10561 aacagctggt cagaattctt tgcgcgcatg atggcaacca cgccgctctc cacgatcatt
    10621 ccaagggctg cattctgatc catgagattt gctcctaaac taaaatcgtt ttttattggt
    10681 attattcgat attttctggg gtggatttgt ttatttcatt caatgaaatg attattcact
    10741 aaatgaaatt atagcatcga aattatgaaa gtcaataatg aattttgtca ctaatcggca
    10801 acagatgtct tgtaccaatc ggacaggagc gaggcaagga tactgggctg gccatcctga
    10861 attatgcaaa ccagttgtgt ccaatggaga ctcttttttg tttagatact tatacccaac
    10921 ccggatgtct gggtgcgcga tttcacagag gagatggaag caaccctgga gcagttggtg
    10981 ggtatggtgg acaagctgaa gaacgacctt taaaagtaaa acttccgaga tagtcttctc
    11041 ggaagttgaa ttttgcttgc acgaaaacat tgggcggtta ggccggcgct tggctcatta
    11101 aaggcctaaa tttataccca tataccacca tgccgcgctc gtcgccgtcg ttgcgcagct
    11161 tgcgggtgac catttcaact ggcgtgccga tttctacttg ttgcccttcc atatcggtca
    11221 gttgagccgt caccaagggc ccttcttcaa gtttgatcaa tgccaccgtg tagggtgcag
    11281 cctcttcaaa gccggctggg gggtcgtaga cggtggtgta agaatatact tcaccgcgcc
    11341 cgctgaactg gtatagagtc ttggcttctt gtccgcactc ggggcacacg tcgcgcggag
    11401 ggaacagttt cgcttcgcag tgtgggcaaa cttcacctac catgctgtaa cgttgtttgc
    11461 gaagtcgcca gtggcgaggg atttccataa gttataactc cttgtcgtaa taaactacga
    11521 taaatgtatt cccattgagc tatcaagaac tatcagattt
//
LOCUS       DBNGSX010000004         4912 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_554216, whole genome shotgun sequence.
ACCESSION   DBNGSX010000004 DBNGSX010000000
VERSION     DBNGSX010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_554216"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="AC16CB_00105"
     CDS             complement(<1..835)
                     /locus_tag="AC16CB_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR3340893.1"
                     /translation="MKHLFAGIEAGGTKFVCAVGSGPQGIRDLTSFPTTQPDETLERA
                     YSFLRPYVHDLAAIGIASFGPVDLRVGSPTYGFITSTPKPGWANFNIVGAIQRAFDLP
                     VGFDTDVNGAALGEWRWGAGQGLDTFVYLTIGTGIGGGALVNSQLLHGLLHPEMGHML
                     LQHDWQSDPFPGICPYHGDCFEGMASGPAIEKRWGTPAETLPPDHPAWELEAHYLALG
                     LDAIISILSPQRIIMGGGVMMQSQIFPLLFAKLQKMLNNYIQVPEILNRIEQYIVPPD
                     LG"
     gene            complement(858..2495)
                     /gene="treS"
                     /locus_tag="AC16CB_00110"
     CDS             complement(858..2495)
                     /gene="treS"
                     /locus_tag="AC16CB_00110"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002620889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HIR3340894.1"
                     /translation="MTGNSLDQDTLWYKNAVFYEVYIRAFYDSNGDGHGDLRGLKEKL
                     DYLQDLGVDCLWLMPIYPSPLKDDGYDIADYYRILETYGTLQDFKDLLDAAHERGMRV
                     ITDLVLNHTSDQHAWFQASRSSRESPYRDYYVWSDTDQKYQEARIIFVDTQKSNWTWD
                     PGSGQYYWHRFYPSQPDLNFDNPAVQQEMLNAVKFWLDMGVDGFRADAVPYLFERDGT
                     NCENLPETHAFLKRLRRFMDENYPGRILLCEANQWPEDVRPYFGDNDEFHMGFHFPLM
                     PRIYMALRKADKQPLEWVLKRTPPIPEDCQWCVFLRNHDELTLEMVTEEERQWMWQEY
                     APDARMRLNLGIRRRLTPLLDNDRRKIDVANSLLFTMPGSPIIYYGDEIGMGDNIWLP
                     DRNGVRTPMQWEDGLNAGFSSATEDQVYAPVIQDDPYGVQWVNVAAQQADPSSLWHAI
                     RKMIKVRKANPTLGWGDFILVESGSPAVAAYWRTYQDDALLILNNLSGSRQAVRVDLP
                     AGSKFEGKDLFSGEKLSLSSLERLELILQPYQYLWLC"
     gene            complement(2528..3874)
                     /locus_tag="AC16CB_00115"
     CDS             complement(2528..3874)
                     /locus_tag="AC16CB_00115"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HIR3340895.1"
                     /translation="MKLTFPEGFLWGVATSSYQIEGAWNDDGKGESIWDHFVHKPYTI
                     LNADTGDKACEHYYRMPEDVELMKSLGLQAYRFSISWPRVLPEGRGKPNSPGLDFYDR
                     LVDNLLSAGIQPVANLDHWDFPQALQAVGGWANRDCTDWFADYAQLMFDRLGDRVSMW
                     STHNEPWVLAFLGHGTGEHAPGICNYAMAYQVAHHLLLGHGKAVQVFRQSARQGQIGI
                     ILNLNYFLPASDREADIAACRRVYMENVSLFLEPLFFGRYPQELFDWIGFHQPKIHDG
                     DLDIIRQPIDFLGVNHYFSESVSHAVNGPVLKARNEPYSAPGWGRTTMNWGINPQGFE
                     AVLLDIKDNYGNPTVFITENGCSFPDVADEHGVVADWDRVNYLRAHMHAAYRALQAGL
                     DLRGYFVWSLMDNFEWAWGYSRRFGLVRVDYPTQKRIPKQSATWYSQVIAANGIDI"
     gene            complement(3875..>4912)
                     /locus_tag="AC16CB_00120"
     CDS             complement(3875..>4912)
                     /locus_tag="AC16CB_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3340896.1"
                     /translation="IPMRYRTLGKSGLIVSELALGTMIFGEKSQRSASPSEAVRIIHR
                     FLEVGGNHIDTSNVYAKGRSEEILGDALRGKRQQVVLATKVRFATGSGPNEAGLSRYH
                     VLRSVEDSLRRLQTDVIDLLYMHSWDPLTPLEESLKVFDTLVTSGKIRYIGVSNFKAW
                     QLMKALGLCDAHGWERFIAAQYQYSLVERSIEHEFTDLCLSEGVGLVPWGPLGGGFLS
                     GKYQPDKRPSSPDEGRLATQPDSDEEAWWRRAIEKNWKILEEVDRVVKAHQGSTYSQV
                     ALAWLLSQPAVASVVIGARTLDQLDDNLASVDLQLDTEEISSLNSVSAVELGYPYRFL
                     QNYGKRSLEGN"
BASE COUNT         1302 a         1337 c         1091 g         1182 t
ORIGIN      
        1 atcccaaatc aggtgggacg atatattgtt cgatgcgatt caagatctct ggtacctgga
       61 tgtagttgtt gagcattttt tgcagtttgg caaacagtaa tggaaatatc tgtgactgca
      121 tcattactcc accccccatg ataatgcgtt ggggggagag gatggaaatg atggcatcta
      181 atcccaaagc taggtaatga gcttccaatt cccatgccgg gtgatcaggc ggcagcgttt
      241 ccgcaggtgt gccccaacgc ttctcaatcg ctggaccgct cgccataccc tcaaaacaat
      301 caccatgata tgggcaaata cctgggaagg ggtcgctctg ccagtcatgc tgcaggagca
      361 tatgtcccat ttcaggatgg agaagaccat gcaggagttg actgttgacc agtgccccac
      421 ccccaatccc tgtgccgatg gtcaggtaga cgaaagtatc taaaccttgg cccgcacccc
      481 atcgccattc acctaatgct gctccattga catctgtatc aaaccccacc gggagatcga
      541 atgcacgttg tatcgcacca acgatattga aatttgccca accgggtttt ggggtcgagg
      601 taataaatcc ataggtaggc gagcccaccc gcaaatctac aggcccaaat gaagctatac
      661 ctatggcagc caaatcatgg acgtatggtc gtaaaaaact gtatgcgcgc tccagtgttt
      721 catctggctg ggtagtagga aaggaggtca ggtcacgtat accctgcggc ccgcttccca
      781 cggcacagac aaatttggtc ccacccgctt ctatccccgc aaataaatgc ttcatgccga
      841 tctcaccgaa tatcgcccta acaaagccat aggtattggt agggctgcag gattaattca
      901 aggcgctcta aacttgacag ggataatttt tcacctgaga acagatcctt gccctcaaac
      961 tttgatcctg caggaagatc gactcggaca gcctgtctcg atcccgaaag attgtttaag
     1021 atcaataacg cgtcatcctg gtatgtccgc caataggcgg ctacagccgg agaaccactt
     1081 tcaaccaaga taaaatcccc ccaacccaat gtcggattcg ccttacgcac cttaatcatc
     1141 ttgcgtattg cgtgccataa cgagctagga tcggcttgtt gtgcagcgac atttacccat
     1201 tgcacaccat aaggatcatc ctggatcacc ggtgcgtaaa cctggtcttc tgttgcagac
     1261 gagaatccag cgttcagccc atcttcccac tgcatcggtg tgcgcacacc attgcgatct
     1321 ggtagccaga tattatctcc cataccgatc tcatccccgt aataaatgat cggagagcct
     1381 ggcatggtaa ataaaagcga gttagctaca tcaatcttac gccggtcatt atccaataaa
     1441 ggtgtcagac gacggcggat acctaaattc aagcgcatgc gcgcatccgg agcatattcc
     1501 tgccacatcc attggcgctc ttcttctgtg accatttcca aagttaactc gtcgtgattg
     1561 cgcaaaaata cacaccactg gcaatcctcg ggaatgggtg gtgtgcgctt cagaacccac
     1621 tccagaggtt gtttatcggc tttacgcaat gccatgtaaa tacggggcat aagcggaaaa
     1681 tggaatccca tatggaattc atcgttgtcg ccaaagtagg gccgtacatc ctcaggccat
     1741 tggttggcct cgcaaagcaa tattctgccc ggataattct catccataaa gcggcgcagg
     1801 cgcttaagga atgcatgcgt ctcgggcaag ttctcacaat tggtgccatc tcgctcaaat
     1861 aaatagggaa cagcatctgc cctaaaacca tccacaccca tatccagcca aaacttgact
     1921 gcattgagca tttcctgttg gacagcagga ttatcaaagt ttaaatctgg ctgggagggg
     1981 taaaagcggt gccaataata ctggcccgag cctggatccc aggtccagtt cgacttctgg
     2041 gtatcaacga agatgatgcg cgcttcttga tacttctgat ccgtgtcgct ccacacataa
     2101 taatcccggt aaggtgattc tcggctggat cgagaggctt gaaaccaggc atgctggtct
     2161 gaagtatggt taagcaccaa atctgtgatc acgcgcatgc ccctctcgtg cgcagcatcc
     2221 agtagatcct tgaaatcttg gagcgtgccg taggtttcaa gaatgcgata ataatcggcg
     2281 atatcatagc catcatcctt caggggtgaa ggatagatgg gcatcaacca caggcaatcc
     2341 acgcccagat cttgcaagta atccagtttt tctttcagac cgcgcagatc accgtgtcca
     2401 tcaccgttgc tgtcataaaa agcgcggata taaacttcgt aaaagacagc gtttttgtac
     2461 cacaatgtat cctgatctaa tgaatttcct gtcaattaaa ttgtttcctt ttcacaatca
     2521 tcatgattta tatatcgatc ccatttgccg caatgacctg actgtaccac gtagcactct
     2581 gtttagggat tcgcttctgt gttgggtaat caacacggac caacccaaac cgccgactgt
     2641 aaccccaagc ccattcgaag ttatccatca aactccagac aaagtagccc cgcaaatcta
     2701 gccctgcctg caatgcccga tacgctgcat gcatatgcgc acgcaaataa ttcacccgat
     2761 cccaatcagc gacaacacca tgttcatccg caacatctgg aaacgaacag ccattctccg
     2821 tgataaagac agttggatta ccataattgt ccttaatatc caacaataca gcttcaaatc
     2881 cctgtgggtt aatgccccaa ttcatggtgg tgcgtcccca accgggtgca gagtacggct
     2941 catttcttgc cttgagcacc ggcccattta ccgcgtgcga gacagattca ctgaaatagt
     3001 ggttgacacc caagaaatca atcggttggc ggataatatc caggtcacca tcatgaattt
     3061 ttggctggtg gaagcctatc cagtcaaaca gctcctgggg atagcgaccg aagaataatg
     3121 gttccagaaa tagactcaca ttctccatat agacacggcg gcatgcggcg atatcagctt
     3181 cgcgatcgct ggctggcaaa aaataattga gattgagaat aatcccgatt tgcccttgac
     3241 gagcactctg gcggaagacc tgaacagctt tcccatgccc taacaagaga tgatgagcta
     3301 cctggtaggc catggcataa ttgcaaatcc caggtgcgtg ctccccagtt ccatgaccta
     3361 gaaaagctaa aacccaaggc tcattgtgtg tagaccacat gcttacccgg tcccccagcc
     3421 ggtcaaacat caattgggca taatcagcaa accaatccgt gcaatcccgg tttgcccacc
     3481 caccaaccgc ttgcaacgcc tgagggaaat cccaatggtc caaattggct acaggttgta
     3541 taccggctga gagcaagttg tccactagcc tgtcgtaaaa atccaggcca ggagaattag
     3601 gcttgcctct cccttcagga agaacgcgtg gccatgatat ggaaaatcgg taagcctgca
     3661 gaccaagact cttcattagt tcaacgtctt caggcatgcg gtagtaatgc tcgcacgcct
     3721 tatcccctgt atcggcgttt aatatggtat atggcttatg aacaaaatgg tcccagatac
     3781 tctccccttt accatcatca ttccaggcgc cctcgatctg gtacgatgac gtagctacac
     3841 cccaaagaaa tccttccgga aaagtaagtt tcacctaatt tccctccaaa gaccgcttgc
     3901 cgtaattctg taagaatcga tatggataac ctagctcgac agcgctcact gaatttaaag
     3961 aggatatttc ttcagtgtca agctgcaaat caacgcttgc gagattatca tcgagctggt
     4021 cgagggtgcg cgcgccaatg accactgaag ccacggcggg ttgagataac agccaagcta
     4081 gagcaacttg cgaatatgtg ctcccctggt gtgctttaac cacccgatcc acctcttcta
     4141 aaatcttcca gttcttctcg atggcacgcc tccaccaagc ttcctcatca ctatcaggct
     4201 gagttgctaa acgcccctca tctggggaag aaggtcgctt atcaggttga tatttcccgc
     4261 tcaagaaacc acccccaagt ggtccccatg gcactaagcc tactccctca ctcaggcaaa
     4321 gatcggtgaa ctcatgttca attgagcgct ccaccaagct gtactggtat tgggctgcaa
     4381 taaagcgctc ccaaccatgt gcgtcgcata atcccaatgc tttcataagt tgccaggctt
     4441 taaaattcga cacaccgatg tagcgtatct tgcccgaagt taccagggta tcgaacacct
     4501 tcaaggattc ttccaagggc gtgaggggat cccaagaatg catgtagagc aagtcgataa
     4561 cgtctgtttg taatcgcctc aagctatctt cgacgctgcg caagacatgg tatctagaaa
     4621 gaccagcctc atttggacca cttcccgtag caaaccgcac tttggtggcc aggacgactt
     4681 gttgtcgttt gccacgcaac gcatcaccga gtatctcctc tgagcgcccc ttggcgtaca
     4741 cattagacgt atcgatgtga ttacccccaa cctccaggaa gcgatggatg atgcgcactg
     4801 cttcagaggg agaagcactt cgctggcttt tttccccaaa tatcatcgtg cctaatgcca
     4861 gctctgacac gatcaacccg cttttaccta aagttctgta acgcataggg at
//
LOCUS       DBNGSX010000005         5154 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_831294, whole genome shotgun sequence.
ACCESSION   DBNGSX010000005 DBNGSX010000000
VERSION     DBNGSX010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5154)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_831294"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="AC16CB_00125"
     CDS             <1..908
                     /locus_tag="AC16CB_00125"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3340897.1"
                     /translation="RIVYRHFPLISIHDKAALSAQAAEAAGMQGKFWEMHDLLFERQG
                     EWVDLSVEEFQEWVIARAEELDLNTEQFSSDLTSQPLADKAQAAWDRGVEIGMPGTPF
                     LLVNQSIWPSNLPMKQGNIAAVIRLELMESRQFSECPPMTIDASKQYVAILHTEKGDI
                     TIELYADKAPLAVNNFIFLARNGWYDEVTFHRVMENFVAQAGDPSGTGFGTPGYAFSN
                     EISPDLKFDKAGIVAMANAGPDSNGSQFFITYAPTPQLDGGYTIFGQVISGMDVAESL
                     TPRDPSQALDLPPGDEIISVEIQER"
     gene            945..2201
                     /locus_tag="AC16CB_00130"
     CDS             945..2201
                     /locus_tag="AC16CB_00130"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HIR3340898.1"
                     /translation="MDNSVYNQPTNINQAHDIHWPSVLQLGFSLLGISILWSGAFILV
                     LIGLIQLLLPAEAQGNSTIPLLLGSSGVMIGLLLVPSVVYPLQRLLGHRPSRLVPTSP
                     WLRPTLLIFALPLLLLAGWWISQQGAALQLILPIFHVPAIGIPILWLSYLAVRDLPLG
                     SPQRAWGLLGSGSVLAPLFILFAEVLVLLAGVITWSIWMSGQPGMLDELSILADRLRQ
                     AGNSPQLIAHIISPYLKDPLVIFSAFTFAAVIVPLIEEAIKPLGVWFLAGSRLTPAEG
                     FTAGILCGAGYALVESLTLSADSGNDWVSLVFARIGTAVVHILTSGLTGWALASAWRE
                     KRYFRLGVTYLGAVLLHGLWNGLTMLIVIADLSLPAAQLGQDLLSQAYQIVPYGLVGL
                     AIAGLATLLWINVRLRRTTDSPQAVV"
     gene            2333..2986
                     /locus_tag="AC16CB_00135"
     CDS             2333..2986
                     /locus_tag="AC16CB_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR3340899.1"
                     /translation="MELITYLIDLFLHLDKHLSIVIQNYGAWTYALLFLIIFLETGLV
                     ITPFLPGDSLLFAAGTFAGLGSLNVIVLFLLLSAAAIIGDTVNYWIGHYIGPRAFSGE
                     IRFLKKEYLDRTHEFYEKHGGKTIILARFVPIIRTFAPFVAGIGAMSYGRFITYNVVG
                     GVVWVAIFTFGGHFFGNLKFVQDNFTFVILAIIFISVLPGVFEFLKERSRSAQKVEA"
     gene            complement(3049..4137)
                     /locus_tag="AC16CB_00140"
     CDS             complement(3049..4137)
                     /locus_tag="AC16CB_00140"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3340900.1"
                     /translation="MKDNLIHRREFLKLAGLAALGTASACAPASETPAQDASWKPVTL
                     PPQPTSMATETTPPTYTATLTQAPEIASVSPCTHGKRVLRVAHLTDIHVQPEGIAPDG
                     MVKALRHAQEQPDPPDLIINTGDSIMDSLNTDKARTQAQWEVFNSILTAECKLPIIHA
                     IGNHDVWGWAHPDQSIRSDPLYGKNMAIEKLGLSHRYYSFDQAGWHFIVLDSNHPPNE
                     VSGYPYIGKLDEEQFAWLVQDVESTPHEMPICILSHIPIVAACEYFDGPNEDSGNWVV
                     PAAWMHIDARRFRQFFLQHLNIRLCLSGHTHQLEALDYLGVRYLTNGAVCGNWWNGSY
                     LDFPPAYVMVDLYQDGSFESISVHYENV"
     gene            complement(4237..4746)
                     /locus_tag="AC16CB_00145"
     CDS             complement(4237..4746)
                     /locus_tag="AC16CB_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340901.1"
                     /translation="MNTRKYLLFALPLILALVLMPTVQARARTVKTTFTGTDTVIRDI
                     NLGKETFPDINLYHIRDNVFITRIEVTDPRVSGEALIKMDANFKLVDPPVFVTGRMWG
                     TFKLSNADGYWEGIIEGVRIKNGYSYFKYVGHGEGAYKGLQLRMRYERLDPDPSVPGT
                     LRGYILETE"
BASE COUNT         1108 a         1399 c         1367 g         1280 t
ORIGIN      
        1 tccgcatcgt ctatcgccat ttcccgttga tctccatcca tgataaggcc gccctatcgg
       61 ctcaagctgc cgaggctgcc gggatgcaag gtaaattttg ggagatgcac gatctgctct
      121 ttgaacgcca gggggaatgg gtagatctta gcgttgaaga gttccaagag tgggtcatcg
      181 cccgtgccga agagttggac ctcaatacgg agcagttttc cagtgatctt accagccagc
      241 ccctcgccga caaagcgcag gctgcctggg atcgaggggt ggagatcggc atgcccggca
      301 cgccgttctt gctggtcaat caaagcatct ggcctagcaa cctgcccatg aaacagggga
      361 acattgcagc cgtgatccgc ctggaattga tggagtcaag gcaattcagt gagtgcccgc
      421 cgatgaccat cgatgcctcc aaacaatatg tcgccatcct gcacactgag aagggcgaca
      481 ttacgatcga gttgtatgca gataaagctc ctttggcggt gaataatttc atcttcctgg
      541 ctcgcaacgg ctggtatgat gaggtgacct tccatcgcgt gatggagaac ttcgtagccc
      601 aggctggcga tcccagcggc acaggctttg gcaccccagg atatgccttc tccaatgaga
      661 tatcccccga cctcaagttc gataaagccg ggattgtggc gatggcaaac gctggacccg
      721 actcgaatgg cagccaattt ttcatcacct atgcaccaac ccctcagcta gatggcggct
      781 ataccatctt tggacaggtc atttctggca tggacgtagc tgaaagccta acaccacgcg
      841 atccctccca ggcgctggat ctgccccctg gcgacgagat catcagcgtg gaaatccaag
      901 agagatagct acaagcaaaa tgtcgcaaat ttagggatat atttttggat aactcggtct
      961 ataaccagcc aaccaacatc aaccaggcac acgatattca ctggccttca gtgctgcagc
     1021 taggcttcag cctgcttggc atatccatcc tgtggagtgg ggcctttatc ctggtgttga
     1081 tcggcctgat acaactgctg ctgcctgcag aggcgcaagg caacagcaca atccctctat
     1141 tacttgggtc gtctggggtg atgatcggac tgctgctggt cccctccgta gtttaccctt
     1201 tgcagcgttt attgggacac cgcccttccc gtttagtgcc aacctcgcca tggctgcgac
     1261 cgaccttgtt gatcttcgct ttgcccctgc tccttttggc tggctggtgg atctcccaac
     1321 agggggcggc attacagctc atcctgccta tcttccatgt cccggcaatt ggcataccga
     1381 tcttgtggct gagttatctg gcggtgcgtg atctgccgct cggttctccc cagcgcgcct
     1441 ggggcttgtt ggggagcggc agcgtactgg cgccgctatt catccttttc gcagaagtac
     1501 tggttctgct ggctggtgta atcacttggt cgatttggat gtcaggacag cctggcatgc
     1561 tggacgaact ctccatcctt gccgatcgcc tgcggcaagc gggcaactcg ccgcagctca
     1621 tcgcccacat catttccccc tatctcaaag atccgctggt gatattttca gcctttacct
     1681 ttgccgcggt catcgtaccc ttgatcgaag aggccatcaa gccattgggg gtatggttcc
     1741 tggcgggctc gaggctaacg ccagccgaag ggtttaccgc aggcatcttg tgcggagcag
     1801 gctatgcact ggtggagagt ctaaccctgt ctgccgatag tggcaacgat tgggtctcgc
     1861 tggttttcgc ccgcatcgga accgctgtag tgcacatcct caccagcggg ctgaccggct
     1921 gggcactggc atctgcctgg agagaaaagc gatactttcg cctgggtgtc acctacttgg
     1981 gggctgtctt gctacacggc ttgtggaatg gcctgaccat gctgatcgtg atcgcagatc
     2041 tttccctacc cgccgcccag cttggacagg atctgctttc ccaggcttac caaatcgtcc
     2101 cctacgggct ggttggcctg gcgatcgccg ggctggcgac cttgctgtgg attaatgtgc
     2161 gcttgcgacg cacaacagat tctccccaag ctgtagtata atcggcggga ctttgatcaa
     2221 gataactctc accccacctg tactggaaaa gggagcaagc ctgaaattgg gaggcctatg
     2281 gccttcagca agctgaactc cctcaggggg aaagcgagat gaaaggattg aaatggaatt
     2341 aattacgtat ttgatcgatt tgtttcttca tttggacaag cacctgagta ttgttatcca
     2401 gaattacggc gcctggacct acgcgctgct tttcttaatc atcttcctgg agacaggact
     2461 ggtaataacc cccttcctgc caggcgactc cctgctcttc gccgcaggca ctttcgccgg
     2521 gcttggatcg cttaatgtga tcgtgttgtt tctgctgctc tccgctgcgg cgattatcgg
     2581 cgatacagtc aactattgga ttggacacta catcggtcca cgggctttca gcggggagat
     2641 tcgcttccta aagaaagaat atctggaccg cacccacgag ttctacgaga agcatggtgg
     2701 caagaccatc atcctggcgc gatttgtgcc catcatccgt acctttgcac ccttcgtggc
     2761 aggcatcggg gctatgtcct acgggcgctt catcacctat aatgtggtgg gcggcgtagt
     2821 ctgggtagcc atatttacct ttggcggtca tttcttcggc aacctgaaat tcgtccagga
     2881 taatttcacc ttcgtcatcc tggcgattat cttcatctcg gtcttaccag gagtatttga
     2941 attcctcaaa gagcgctccc gctctgccca gaaggtagag gcgtaaaagc cctaacctcc
     3001 ccccatccac gatctctttt agcggcgctg cacacagcgt cgcttttttt atacattttc
     3061 ataatggaca gatatgcttt cgaaagaacc atcctggtac agatccacca taacataagc
     3121 cggggggaaa tccaaatagg atccgttcca ccaattcccg cataccgccc catttgtcag
     3181 atagcgtacg cccaggtaat cgagcgcttc aagctgatgg gtgtggccgc tcaagcacag
     3241 ccggatgttt aggtgctgca ggaaaaactg cctgaaccga cgcgcatcaa tgtgcatcca
     3301 ggctgcgggt acaacccagt tgccgctatc ctcgttaggg ccatcgaagt attcgcatgc
     3361 agccacaatg gggatgtgtg aaaggatgca gattggcatt tcgtggggcg ttgactcgac
     3421 atcttgaaca agccacgcga attgctcctc gtctaatttg ccaatatatg gatatccaga
     3481 cacctcattg ggcgggtgat tgctgtccag aacaataaag tgccacccag cttggtcgaa
     3541 actgtagtag cggtgagaga ggcccagttt ctcgatcgcc atattcttac cgtatagcgg
     3601 gtcacttcga atgctttgat ctggatgagc ccacccccat acatcgtggt ttccaatggc
     3661 gtggatgatg gggagtttac actcagcagt caaaatacta ttgaatacct cccactgtgc
     3721 ctgggtgcgg gctttatccg tattaagtga atccatgatc gaatcgcccg tgttaataat
     3781 gagatcgggt gggtcaggtt gctcctgtgc atggcgaagg gctttaacca tgccgtcggg
     3841 agcgattcct tcgggctgaa cgtgaatgtc cgtcaggtga gcgacgcgca gaacgcgttt
     3901 cccatgcgtg cagggtgata cgcttgcgat ttcaggcgcc tgtgtgaggg tagcagtata
     3961 ggtaggcggg gttgtctcgg ttgccattga tgtgggctga ggaggcagcg ttaccggctt
     4021 ccaggacgcg tcttgtgcag gagtctctga ggcaggagcg cacgcactgg cagtccccag
     4081 agctgccaat ccagccagct tgagaaattc cctgcggtga atcaaattgt ctttcattac
     4141 ctatttcctt gttatggata ggtctggtga cataaagaga tcaaaacgga gtggcgcggg
     4201 gaactcccgc gccactccgt tcttgtggag cgaaggtcat tcagtttcaa ggatataacc
     4261 ccggagggtg cccggcacac tcgggtcggg atccaggcgt tcatagcgca tgcgcagctg
     4321 taaacctttg tatgcaccct ccccatggcc tacgtacttg aagtaggaat acccgttttt
     4381 gatgcgaact ccttcgatga ttccctccca atagccgtcg gcattggaaa gtttgaaggt
     4441 accccacatt ctaccggtga caaaaaccgg gggatcaacc aacttgaagt tagcatccat
     4501 tttgatcaag gcctcgccgc tgactcgcgg gtcggtcact tctatcctgg tgatgaacac
     4561 attgtcgcga atgtgataaa ggttaatgtc ggggaaagtc tctttgccca gattgatatc
     4621 cctaattacc gtgtcggtcc cggtaaaggt tgtcttgacc gttctggcgc gcgcctgtac
     4681 agttggcatc aacaccaaag ccaggatgag cggtagcgca aacaacaggt atttgcgcgt
     4741 gttcattttc ctcttccttt catgattttc ccattgtgta tcaaaatgat tgtcaaaatc
     4801 gatgcagtgc tcgtgaatcg cgttcggcga tccttatctg ccaaagatgt agagaaatca
     4861 ggtgtgcatc aaccaaatac atctctcctt tccggtgcgg ctcaggcatc agcttccgct
     4921 tacctgttgg accggcatca gaaatattac agtgtaaccg cgatcgccat gcattcccga
     4981 tcggcaggaa aggggctttg agcagcagac catttagacc ggcccatcct ccacctggat
     5041 ggtatgcgtt tcttgcttgc ctggaccgag ctcctcgaat aattggaagg tgcccagaaa
     5101 ggaggatacc cggtcgtgga tactatgagg tccagcatat ctttgcccgc atcc
//
LOCUS       DBNGSX010000006        19005 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1800957, whole genome shotgun sequence.
ACCESSION   DBNGSX010000006 DBNGSX010000000
VERSION     DBNGSX010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 19005)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19005)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19005
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1800957"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1364
                     /locus_tag="AC16CB_00150"
     CDS             <1..1364
                     /locus_tag="AC16CB_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HIR3340902.1"
                     /translation="ELRGFERFCVGRPVEEMPLLTNRICGVCPEAHHMAAAKAADAVY
                     HVDPPRTAKMLRELLYSAFYVTDHTTHFYALAGPDFVMGPESPVAQRNILGVIHKVGL
                     EVAGKVIQARKYGHTVIEMIGGRKVHPCTSIPGGLTKGITEEQRDEIEKMGRWAIEFA
                     QFTLQLFNDIVLGNKQYVDLILGDIYTHRTYSIGTVDENNKVNFYDGKVSVVSPDGER
                     IGKYAPNEYTEWIAEHVEPWTYLKFPYLKKIGWKGFVDGKDSGVYFATPLSRLNAAEG
                     MATPLAQEEYEKFFTTLGGKPVHQRLATHWARLIELLYAAERWVELATDPEITSNNFH
                     IAPTETPTEGVGIVEAPRGTLTHHYKTDNRGVLTEVNLIVGTTNNYAPISMSIKKAAQ
                     SLIKRGNVVTEGMLNMVEMAFRAYDPCFGCATHTLPGQMPLQVSIRDSNGNLVDILKQ
                     NCP"
     gene            1419..1913
                     /locus_tag="AC16CB_00155"
     CDS             1419..1913
                     /locus_tag="AC16CB_00155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HIR3340903.1"
                     /translation="MKTLIVGLGNPILGDDGVGWHVARALLKRLKEGYFDIEQPDIKC
                     LSLGGLSLMENLVGYDHVIIIDAINIGRGHLGSIYKFMLDDLPDPNTGHTSSAHDTSL
                     QSAIKMGSSIGLKLPASIQVIAIESQIELNFSEDLSPPVAKAVPLAVELLMNELVKSG
                     TLKG"
     gene            1951..2418
                     /locus_tag="AC16CB_00160"
     CDS             1951..2418
                     /locus_tag="AC16CB_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR3340904.1"
                     /translation="MISPETLRRYPHFAKLTEAQLRALAMIADEVNYTSGSIILEEGK
                     DAEWLYLLVEGCIDLSYKSEESYHPKATKIFQVGEINPGELFGISALIDPFKYNATAT
                     TSEQSTIVKFNAKSLRTISDLDCTLGYTLMQQIAKAVMERLTHTRIQLAAARD"
     gene            complement(2518..3237)
                     /locus_tag="AC16CB_00165"
     CDS             complement(2518..3237)
                     /locus_tag="AC16CB_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340905.1"
                     /translation="MKYITSFIIIAMLTLMPLSSGIFTQSASASATYYTGHPTLTILS
                     VKRDSSVTFYANNLPKHDAFRVRMGLMGTRGVRGTIVDSFETGSGGRKKFTFSIPSKY
                     HGDYRIAIRIESKTGSGYFAYNWFYNNTTGAGTGGKSGRKGYSGYPTMKILSVVRNNS
                     VTVRLRNLPPRDEFKVLMGRYGTRGINGIKVISIDTEKGGNQTMTFSIPSELRGLRRI
                     AIRIQSKTGSGYYAYNWFYNR"
     gene            3410..4336
                     /locus_tag="AC16CB_00170"
     CDS             3410..4336
                     /locus_tag="AC16CB_00170"
                     /inference="COORDINATES: protein motif:HMM:NF018677.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HIR3340906.1"
                     /translation="MDNPFFNQLSSCSTLSISVNEQLYGTAPKTNLWVVVEYTNPFGS
                     KALIESLIPERVQSHLTNLQKIVPGTRLVLIRRDQPRTDNQIAVFASISSADQQYLYE
                     FHLSNYDEILSLDLQSLITGKIDIRENIRVDPIFLVCTNGKRDPCCSRLGYPVYQAMA
                     SQQGDSVWQTSHVGGHRFAANVICLPHGIYYGRVNPENAVSLMSDYQNQRLTPYLYRG
                     RGCYDSQVQAAEYFFMESTSIESIDALQLIGSEEVGENRWEIKFLSRADKTRYKFIVA
                     AEKSAFEIFESCSKPDKSSSRLQYHLVEWSQA"
     gene            4333..4953
                     /locus_tag="AC16CB_00175"
     CDS             4333..4953
                     /locus_tag="AC16CB_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HIR3340907.1"
                     /translation="MTNLTIYQIYDLLNRPRPLWLVKINLSETSLWMADLSECNLSWA
                     DLTRCDLSWAKLIKTDLSRANLSYANFYEAHLTHANLTEANLNRANLTRANLEKTNLY
                     HAVATEANFSRANLSRSNLKQANLSGAIFSRADLSKSDLINCNMNGAILTHTNLFEAN
                     LQGVDLKGANLENADLTASNLADANLHGANLTGANLTDAILPEGMK"
     gene            5000..5566
                     /locus_tag="AC16CB_00180"
     CDS             5000..5566
                     /locus_tag="AC16CB_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340908.1"
                     /translation="MSADQNDKRVGFLPFHAINEFMTEEYRLQVIRDVLTGLPNLPED
                     HRNKINQLTRKLVKVPGFRNSVKAPLPIRIKSTVPAFEKKPDLAAALLAAWADAKAEL
                     REKVYALLKSRNWELLPLEIDRTKLPGFMTIWPGGENFEILNQAFGETYPDFSTTNDD
                     VSLMVVWISTRLPYQVTDEGEVEVQTIR"
     gene            complement(5572..6681)
                     /locus_tag="AC16CB_00185"
     CDS             complement(5572..6681)
                     /locus_tag="AC16CB_00185"
                     /inference="COORDINATES: protein motif:HMM:NF027787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HIR3340909.1"
                     /translation="MDPYNSNPKRYSDRILIYILVAILLAILCMVCLGSGILIRRYAF
                     PDVKSTRTALAELFGTITPLVTPSLQLDTPQASPTETPKPLPTQTQVPPKPTYSPPVW
                     LEPPPGKIVYACFVNGYDQICKMKADGSGIIQLTDEESTNFYPSFDPDGERIVFSSRR
                     DGNFEIYIMDVDGSNVHKLTDNVGNLYAPEISPNGKRIVFTAESGGKQAIWIMKIDGG
                     NARPISSDDIYGIDPTWSPSGDRIAFASIIGGATQLFIVNADGTKLKQVTKGISGIGG
                     RSTWSPDGEWLAFYAGPKGDRDIYMINVDDKELRKLTDGGDNLGPSFSPDGEWIAFTS
                     YRDGNNEVYVMRSDGSNATRLTGELRSDWQPRWGR"
     gene            6771..7829
                     /gene="rtcA"
                     /locus_tag="AC16CB_00190"
     CDS             6771..7829
                     /gene="rtcA"
                     /locus_tag="AC16CB_00190"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HIR3340910.1"
                     /translation="MLEIDGSIGEGGGQVLRSALTLSILTGTPMLIKEIRARRSTPGL
                     MPQHLKAVDAAAAVSRAHVEGAFKGSTSLKFEPGQLRSGRYRFDIGTAGSTSLVLQTI
                     FLPLSKASSASSVIITGGTHVPWAPSFHFLAWHWLEWMKKLGYNAEISLDQAGYYPQG
                     GGRISATIRPCDGQVPLPSVKRGQLLRIRGLSAVSNLNIDIAERQKRRAIQRLHPHFD
                     NVRIKIEQLPSRFKGTMLFLLAEFQSDDTQNGYTQCCYYSLGKLGKPAEAVADEAVDA
                     LLDFLNTDAAIDQYLADQLLLPLAFATSQSAIRTSQITPHLLTNAEVIRQFLSAKIEI
                     DSDIGKPGLIRVIPNPSS"
     gene            7837..8931
                     /gene="dinB"
                     /locus_tag="AC16CB_00195"
     CDS             7837..8931
                     /gene="dinB"
                     /locus_tag="AC16CB_00195"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HIR3340911.1"
                     /translation="MTRKILHLDLDAFFCAVEEQRDPSLIGKPFAVGGRPEHRGVVAS
                     CSYAARKYGVRSAMPMSRALRLCPNLIIVSSRHRIYSDVSKQVMVRLRQLTPLVEQIS
                     IDEAYLDVSELPDSGMVLAQRLQADIKEQLGLPCSLGVATNKLLAKTANDVGKSAARS
                     TGPPNAITVVPPGDEEAFLSPLPVQALWGIGPKTAERLKEIGVITIGDLARLPEQELV
                     SMLGKNGYDLAKRARGIDDRPIITEHKIKSISQETTFARDIRDKFTLRQTVLELSEKV
                     GRRLRAYSLSATTVKLKIRWHDFTTLTRQMTLSHPTDQDSEISLIALQLLDNVWKPSQ
                     LVRLIGVGVSGLKPPHHQLSFWDASDQKDL"
     gene            8928..10049
                     /locus_tag="AC16CB_00200"
     CDS             8928..10049
                     /locus_tag="AC16CB_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340912.1"
                     /translation="MNLIQINTKDKRQVNKFLDLPFRLYAYVPQWVPRLMPEARRMLD
                     TQRNPFFKHGEAAFFLVLDDQETPQGRLAILNNHKYNEYNQEKTAFFYLFECENNQQI
                     SNLLFEAAFAWVRLRGLDKIVGPKGFTVFDGLGMLVKGFEHRPAFGLPYNMPYYPELV
                     EAVGFQSKGELVSGYLNSKMQFPAKIHQVAELLQERRGLRVARFKTRRDLRAFVPKLK
                     ALYNGALASTDGNAPLSDEEVKALADQMIWFANPRLLKIVYKGDQPVGFLLAYPDISA
                     AVQRTRGRIFPLGWIDLLLELRRTKWININGAGMLEGYRGLGGTALLFSEMHKSVTEE
                     GYQHADLVQIGTENERMLRELRGLGIDFYKTHRMYSRTM"
     gene            10314..12041
                     /locus_tag="AC16CB_00205"
     CDS             10314..12041
                     /locus_tag="AC16CB_00205"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_function: GO:0004574 - oligo-1,6-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009311 - oligosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HIR3340913.1"
                     /translation="MTELKWWQRAVFYQIYPRSFADGNGDGIGDFPGMIQKLDYLQDL
                     GIDAVWLSPHYPSPQFDCGYDITDYKGIAPEYGTLSEFNRFIAGAHQRGIRVILDLVL
                     NHTSHEHPWFIESRSSRHNPKRNWYVWKDGKVDDNGILQPPNNWNSTFDGIAWEFDPP
                     TNQYYYHYFLKQQPDLNWHNPEVKREMWDSVRFWLDLGVDGFRLDAISTIYERPEMPK
                     HPLDMSLASLRRILRDSRTPEEHAQARRLQKLMFQYQNHQPGLHELMQELRSVIDEYD
                     DCVLVAEDDDIAYMGNGDNELHLVFNFPLMRTGRLTPAWVRANQKERLTTLASISPSA
                     WPCNTLGNHDSPRVYSHFGDGKNDQQLARISLALMLTLRGTPFLYYGEEIGMTDNYLE
                     HIDDFRDLLGTWQYQAEIDEFGTPPDLALQYAAKLTRDKNRTPMQWENSPNSGFSPAN
                     VRTWLPVNTNYDSGVNVADQVADPASLLKFYQRILSLRKNNPALIGGDYLPLHEHSDD
                     YLAFLRRAEQQTCLVVLNMVAKHLRIHFDLEGVSAQLLFSSQERQTNWLDLNLIQLSP
                     FEILIAEMI"
     gene            12258..14918
                     /locus_tag="AC16CB_00210"
     CDS             12258..14918
                     /locus_tag="AC16CB_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR3340914.1"
                     /translation="MDKETILVVDDNHQISRFLAHELLPSLGYSAMVARNGKSALEAL
                     RGEKVALMLLDLQLPDMSGLDVLKQVAKEGLNVPTILATAHGSENIAIESFRLGVEDY
                     LIKPIDPDRLNDAITHALSESRLRREKAVLTNKLREQLSWQSVLFKIGQSVISSLEAD
                     EVLRRIVEAGVLLTGAEEGFLALLDEQTDRLFLRASKNIDQEKSKTMRLPITDNLIAK
                     VLQTRKPVRTITQTAEDPLIKLSTGFLVRSLLHVPIISRGKALGVLTMVNHSHQKPFL
                     DKDESLMTSLASYASIALDNAGLYHRAQQEIIERRKIEEALRESEERYALAMRGSNDG
                     LWDWDLRSNEIYFSPRWKSMLGYSEKEITNSPQEWFSRVHPDDIDKLRIDIETHLTRN
                     SPHFENEHRLQHKDGDYRYILSRGIAVSDANGMAQRIAGSITDITERKAVEQKLLQYA
                     FYDKLTGLPNRALFVDHLSLAIERAKRRPDFQFAVLFMDLDTFKDVNDSLGHMVGDEL
                     LVSVARLLQSRMRSTDTVARFGGDEFVILLDDIHDKFNAQQVSEWIQSALAEPFILNG
                     QEVYISASIGIVLSETGYQRSEEVLRDADIAMYNAKANGKSRFEIFNPEMRTRLLDRL
                     ELANDLRRAIENQELRVHYQLIVSLNNGRITGLEALVRWQHPQRGLLAPIEFIPLAED
                     TGLIIALDRWVLREACRQMHSWKLENPMFSDLTISVNISGKQLSQGDFISFVEHTLEA
                     ADLDPKYLCLEITESVVIGNKEITVEMCNKLRQLGVHIHIDDFGIGYSSLSYLSDFPV
                     NALKIDRSFISKMSEGKNEADIVQAIVTLSQRIGVDVIAEGVETSGQLKKLQAMGCEY
                     GQGFYVSKPLAGDEAKKLVLQSLVVSVSPS"
     gene            15084..15893
                     /gene="fdhD"
                     /locus_tag="AC16CB_00215"
     CDS             15084..15893
                     /gene="fdhD"
                     /locus_tag="AC16CB_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HIR3340915.1"
                     /translation="MKPAKLPIQYHQVSPSQWDKVDTEVIVETPVSLTVNGEAWLTFM
                     CTPVDLEAMAVGFLFNEGLIKSADEVADVRLCDTGDNLDVWLHRSVDKPDKWSRTSGC
                     TGGMTSVESNITSPAITNGGVLYADRVGQLIAQLFESQQLYRASGGVHTSALSDGQRI
                     WIIAEDIGRHNTLDKIAGHYLLEGLQPQKRIILTTGRISSEMLQKSARLNSSVVISRT
                     SPSSLSIELAQKWGITLIGYARRNKFNIYTHPERIVFAPEGKKAKEEPIFV"
     gene            15931..17685
                     /locus_tag="AC16CB_00220"
     CDS             15931..17685
                     /locus_tag="AC16CB_00220"
                     /inference="COORDINATES: protein motif:HMM:NF013662.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HIR3340916.1"
                     /translation="MTNHHIRFKPPDPQIAALVMAHAGKPEAVLEVLKGVQDLYRGLS
                     QENIQDVALSLNIPAERAYGVASFYSMLSLEPVEGKIIRVCDGPVCWLKRASEVQLAL
                     ESEAGRAGYTIERSSCLGLCDRAPAALVVQEQAGPVNPGDARKLVQGERGVSIDYSHP
                     RRGEVRVMMAQAGMVDPDSLQSALSHGVYEGLKKALSLTPEQVVAVVENSGLTGRGGA
                     GFPVGRKWRFVAQSGRMPRYVICNADESEPLIFKDRVLIDTNPHQILEGMAISGYATG
                     SHEAFIYIRGEYAAQAARLERAISQAEEANLLGESILDSGFSFHIHVHMGAGAYICGE
                     ETALIESLEGKRGEPRARPPYPPSYGYMGQPTLVNNVESYAAVPAILQNGAQWYRSIG
                     NNNGGTSQPGITPGTKLYMILGHINQPGLFEAPFGLTLRQVIDEFGGGLLPGSQFNFA
                     LTGGAAGTIVPPSLLDVSIDYASAAMGVSLGAGAFLICDHTVSPIAFLRELLHFFTVE
                     SCGKCTPCRIGTQQSLLILNRMTLGEGKTGDVQQLRQLADLMQQASFCGLGQSVAIPV
                     KSALTHFAKQFQSSEQAQ"
     gene            17705..>19005
                     /locus_tag="AC16CB_00225"
     CDS             17705..>19005
                     /locus_tag="AC16CB_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:18791..18793,aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR3340917.1"
                     /translation="MAINVTINEQTINAKEGQTILEAAQDHGITIPTLCYHKDLSPVG
                     SCRLCLVEVDKWRGQIAACTTQVAQDMIVRTETPALIQSRRLILEMLLQNYVDAGYAS
                     GDEKHDEFMAWVRHYQVALPEGSIPAQRYRVDSDPNPFLWVDLNKCILCTRCVRACAE
                     IQGRFVWGVGQRGYEAHIIAGANTDLLNARCESCGACVTYCPTGALDHKMSINLGHPD
                     KVVTTTCTYCGVGCQLDLNVKDNHVIRVTSNPNAPVNGNHLCVKGRYGYDFVHHPDRL
                     TQPRVRSYLLQDDSKPKGDLGEWVEVDWDTALEITARKLTHTRDTFGPDSIGVLTSAK
                     CLNEENYLMNKFTRQVIGTNNIDHCARLUHSSTVAGLATSYGSGAMSNSMDDIANHAQ
                     SMLVIGSNTTEQHPVFGSMIRQAILKRKAKLVVADPRKIDLT"
BASE COUNT         5107 a         4650 c         4408 g         4840 t
ORIGIN      
        1 ccgaactgcg tggtttcgag cgcttctgtg ttggtcgccc ggtagaggaa atgcccctac
       61 tcactaatcg catctgtggt gtatgtccag aagcgcacca catggcagca gctaaagctg
      121 ccgatgctgt ctatcacgtt gatcctccgc gaaccgcaaa gatgcttcga gagctgctgt
      181 acagtgcttt ttacgtcacg gatcacacca cacattttta cgccctggct ggtccagact
      241 tcgtaatggg tccggagtcc cctgttgctc aacgtaacat actgggagtg atccataagg
      301 ttggactgga ggtagctggt aaggttatcc aagcgcgcaa gtacggtcac accgtaattg
      361 aaatgatcgg cgggcgcaaa gtgcatcctt gcacctcaat tccaggtgga ttaaccaagg
      421 gtataactga agaacaacgc gacgagattg agaaaatggg ccgatgggca atcgagtttg
      481 cccagtttac cctccagtta ttcaacgata ttgtccttgg caataaacag tatgtcgacc
      541 tgattctagg cgatatctac actcaccgta cctattcgat tggaacggtg gatgagaata
      601 acaaagtgaa tttctacgac gggaaagtga gcgtggtttc acccgatggc gagcggattg
      661 gaaaatatgc tcccaatgaa tacaccgagt ggattgcaga gcatgtagaa ccctggactt
      721 atctcaagtt tccatacctg aaaaagatag gctggaaagg atttgtggac ggtaaggatt
      781 caggtgttta ctttgctacg ccgctctccc gattgaacgc tgctgaggga atggccaccc
      841 cgcttgcgca ggaagaatac gagaaattct ttactacact tggaggcaaa ccagttcacc
      901 agcgacttgc aacccattgg gctcgcctga tcgagttact ttacgccgcc gaacgttggg
      961 tagagctggc aaccgatccc gagatcactt ccaacaattt ccacatagct cccactgaga
     1021 caccaaccga aggggtaggg attgtcgagg cgccgcgcgg cacactaacg catcactata
     1081 aaaccgataa tcgcggtgtg ctaaccgagg tgaacttaat tgttggcaca acgaacaatt
     1141 atgcacctat tagcatgtca atcaaaaaag ctgcccaaag cttgattaaa agaggcaatg
     1201 tggtaacaga aggtatgctc aacatggtgg agatggcctt ccgagcttat gatccctgct
     1261 tcggttgcgc gacgcacaca ctacccggtc aaatgccatt gcaggtctct atccgagatt
     1321 ccaatgggaa ccttgtggat atcctcaagc aaaattgccc gtaaattgtt gaaggttgag
     1381 gttcgcttgt tgatctcaac cttcaactct gatatcaagt gaaaacactg attgtaggct
     1441 taggcaaccc cattttaggg gatgatgggg tgggatggca cgtcgccaga gcactcctta
     1501 agagactcaa ggagggttac ttcgatattg agcaacctga catcaaatgc ttatcgcttg
     1561 gcggcttaag cttgatggaa aatctagtgg gttacgatca cgtaataatc atcgatgcta
     1621 tcaacattgg tcgtggacac ctggggagta tttacaagtt catgttggac gatctaccgg
     1681 accctaacac cggacataca agctctgcac atgatacctc tctccaaagc gcaatcaaaa
     1741 tgggaagttc aattggcttg aaactccctg cctcgataca ggtaattgct atcgaatcgc
     1801 aaatcgaatt aaacttttcc gaagatctca gccctccagt tgctaaagcc gtaccacttg
     1861 cggtcgagct actaatgaac gagctagtca aatcaggtac tcttaaggga taatcaatag
     1921 acttacaatt cagatggagg tatatatctt atgatttccc ccgaaactct ccgccgttat
     1981 ccccatttcg ccaagctcac cgaagctcaa ctacgggcat tagctatgat cgccgatgaa
     2041 gtgaattaca cttctggatc aatcattctt gaggaaggca aagatgctga gtggttatat
     2101 ttattggttg aagggtgcat cgaccttagt tacaaatcag aggaatccta ccatcccaag
     2161 gctacaaaaa tttttcaggt gggtgaaatt aatccagggg agctcttcgg tataagcgcc
     2221 ctgattgacc cattcaaata caatgccacc gctaccacct ccgaacaaag cacaatcgtt
     2281 aaatttaatg caaagtcact acgtactata tccgatctgg actgtacctt aggctacaca
     2341 ttgatgcagc aaatcgcgaa agccgtgatg gaacggctaa ctcatacacg catccaacta
     2401 gctgcggcgc gagattagaa gttatctttg tgaacgaatg agatttaacg aattatccag
     2461 ggtcatgccg ctgtatctac tcagcatgac cctctaaccg attgttaagt aactttttta
     2521 tcggttataa aaccaattgt aggcgtaata accggatcca gtcttgctct ggatacgtat
     2581 agcaatccgc ctcagaccac gcagctcgga ggggatgctg aatgtcatcg tctgattccc
     2641 tcctttttcc gtgtcgatac tgatcacttt tatgccattg atgcctctcg tgccatagcg
     2701 gcccattaat actttgaact catcacgggg aggcaggttt ctcaaccgta cagtgacaga
     2761 attgtttctg accaccgaca ggattttcat tgtggggtaa cccgaatagc ctttgcgacc
     2821 ggattttcca ccagtgccag caccagttgt attgttgtag aaccaattgt aggcgaaata
     2881 accggatccg gtcttgctct cgatgcggat agcaatccga taatccccat gatatttgct
     2941 ggggatacta aaggtgaact tcttcctgcc gccgctgcca gtctcaaagc tatccacaat
     3001 cgttccgcgg acaccgcgtg tgcccatgag ccccatgcgc accctgaatg catcgtgctt
     3061 tggtagatta ttcgcataga atgtgaccga agaatcacgc ttaacgctga gaattgtcag
     3121 tgtagggtgg ccagtatagt aggtcgccga agcgctggcg ctttgagtga agataccaga
     3181 gcttaagggc ataagggtta acatggcaat tatgatgaac gaggtgatat acttcatttc
     3241 aatctcctta tgataaacat gcagaactta aaaaaccttc gtcggcaacg aaacacctgt
     3301 atactatgat tgtattctaa tttggttaat cctcacttaa caattatttt agattttatc
     3361 agaaaagtgt aaaaaatggt tacataagcg agtaaaatat taaatcatta tggacaatcc
     3421 atttttcaat cagctatcga gttgttctac attatctata tcggtaaatg aacagcttta
     3481 tggtacagct ccaaaaacaa acctgtgggt ggtggtggaa tataccaatc cgtttggtag
     3541 caaggctttg attgagagtc ttatcccaga gcgtgtacag tcacatctaa caaatcttca
     3601 aaaaattgtc ccggggacac gcctcgtcct catccgacgc gaccagccca ggacggataa
     3661 ccagattgct gtcttcgcca gtatctcttc tgcagatcag caatatttat acgaattcca
     3721 tctatccaat tacgacgaaa tactctcttt agatctacaa tctctcatca caggcaagat
     3781 cgatatacga gagaatatcc gagtagatcc aattttcctg gtctgtacga atggcaaacg
     3841 cgacccctgc tgttcaaggc tgggttatcc agtctatcaa gctatggcct ctcagcaagg
     3901 cgattctgtt tggcaaacat ctcacgtcgg tggccatcgt tttgccgcaa acgtaatctg
     3961 cttaccccac ggcatttatt atggcagggt aaatccagaa aacgcggtct cactgatgag
     4021 cgattaccaa aaccaaagac ttactcctta tttatacaga ggtagggggt gctacgactc
     4081 gcaggtccag gctgctgagt atttctttat ggaatcaaca tccatagagt caattgatgc
     4141 cctccaatta atagggagcg aggaagttgg tgaaaaccgt tgggaaataa agttcttatc
     4201 ccgagctgat aaaacgcgct acaaattcat tgtggctgca gaaaaatccg cttttgagat
     4261 ctttgaaagc tgtagcaaac cagataagag ttcatctcgc ctgcaatacc acctggtgga
     4321 gtggagtcaa gcatgacaaa cctcacgatc tatcaaattt acgatttgct taaccgccca
     4381 cggccactct ggctagtaaa aatcaacttg agcgaaacga gtttatggat ggcagacttg
     4441 agcgaatgca acttgagttg ggctgacctc acccggtgcg atctgagttg ggcaaaactt
     4501 attaagactg atctatccag ggccaatctt agttatgcaa acttttacga agctcaccta
     4561 acccacgcca accttactga agccaacctg aatcgagcaa accttacacg ggctaatttg
     4621 gaaaaaacta acttgtatca tgctgtcgct accgaagcga attttagccg tgctaactta
     4681 agtcgatcca atttaaaaca agccaacctt agcggtgcaa tatttagccg ggcagatcta
     4741 agtaaatcag atttgatcaa ctgtaatatg aacggcgcca tcttgaccca tacaaacctt
     4801 ttcgaggcaa acctgcaagg tgttgatcta aaaggtgcta atctagagaa tgcagatttg
     4861 actgcatcta acctggcaga tgccaatctc catggggcaa atttgaccgg ggctaacctg
     4921 acagatgcca tactcccaga aggcatgaaa taagacaggg tattaataat cacttctaat
     4981 tatcattagc aggagagtta tgtcagctga tcaaaatgat aagagagttg gattccttcc
     5041 tttccacgcg atcaacgaat ttatgaccga ggaatatcgc ctgcaagtga ttcgcgatgt
     5101 gctgacaggg ttgcccaatt tacctgagga tcaccgaaat aagataaatc aactgactag
     5161 aaagcttgta aaagtcccgg gcttccgcaa tagcgtgaaa gccccattgc ctatacggat
     5221 aaaatctact gtccctgcat ttgaaaaaaa gcctgattta gcagccgctc ttttggcagc
     5281 ctgggcagat gcgaaagctg agcttcgaga gaaggtttac gcactgctca aatcacgcaa
     5341 ttgggaattg ctccccctgg agatagaccg caccaagcta ccaggtttca tgacaatttg
     5401 gcctggtggt gaaaattttg aaattctcaa ccaagccttt ggtgagacgt atcctgattt
     5461 cagtacgaca aatgatgatg tgtcattaat ggtggtttgg ataagcaccc ggctcccata
     5521 ccaggtcacg gacgagggag aggtggaggt gcaaacgata aggtgactct actatcgacc
     5581 ccaacgaggc tgccaatctg agcgcaactc acccgtcaga cgagtcgcat ttgaaccatc
     5641 gcttctcatc acatacacct cattgtttcc atctcggtac gatgtaaacg caatccattc
     5701 accatcgggg gaaaagctcg gtcccaggtt atcaccccca tcagtcagct tcctaagttc
     5761 cttgtcatcc acatttatca tataaatgtc gcgatctcct tttggtccag cataaaaagc
     5821 cagccattct ccatcaggag accacgtgct acgcccgcct attccagata ttccctttgt
     5881 gacctgcttt aattttgtgc catcggcatt gacaataaat aactgtgtcg cgccaccaat
     5941 aatggaggca aacgcaattc tatctccact tggcgaccag gttggatcaa tcccgtaaat
     6001 atcatccgag gatattggac gagcattgcc tccatcgatt ttcataatcc agatcgcctg
     6061 tttcccgcca ctctcggctg taaatacgat gcgttttccg tttggtgaga tctcaggagc
     6121 atatagattc ccgacattgt cagttaattt gtgaacattg ctgccatcaa catccatgat
     6181 gtagatttca aaatttccat ctcttcttga cgagaaaacg atccgttccc catcgggatc
     6241 gaaggaagga taaaaatttg tgctttcttc gtcagtcaat tggataatcc cgcttccatc
     6301 cgctttcatc ttgcagattt gatcatatcc atttacaaag caagcataca cgatcttacc
     6361 gggtggaggt tctaaccaaa ccggaggtga ataagttggt ttaggtggaa cttgtgtttg
     6421 tgtaggaagg ggtttgggcg tttcggttgg agaagcttgg ggtgtgtcaa gttgcaacga
     6481 tggtgtgaca agcggggtaa tagttccaaa tagctccgcc agcgcagtgc gggttgactt
     6541 cacatccgga aaggcgtatc ttctaatgag tatccctgac cccaggcaaa ccatacataa
     6601 aattgctagc aggattgcaa ccaagatata gatcaatatt ctgtcagaat atctctttgg
     6661 attggaattg tacgggtcca tatgaataca cacttcgcat acaggcagga tgtaattccc
     6721 aaattataac aatactggaa ttgcctaaac ctttgggtta gaataccctt atgttggaaa
     6781 ttgatggatc aatcggcgaa ggcgggggac aggtacttcg ctctgctctg acactgtcga
     6841 tattaaccgg aacacctatg ctcatcaaag aaatccgtgc acgccgatcg acacctggtt
     6901 tgatgccgca acatcttaaa gctgtcgatg ctgcagctgc tgttagccga gctcatgtag
     6961 aaggggcctt caaaggttcg acttctttga aatttgagcc aggccagctg cgatctggtc
     7021 gttaccgatt cgacatcggt accgctggat caacctcgct agtattgcaa accatttttc
     7081 ttcctctgag caaagccagt agcgcctctt ctgtcatcat cacaggcggc actcatgttc
     7141 cctgggcgcc tagcttccat tttcttgcct ggcactggct ggaatggatg aaaaaactcg
     7201 gctacaatgc agagataagt ttggaccagg caggatatta tccacagggt ggtggtcgca
     7261 tatcagcgac gatccgcccc tgcgatgggc aagttcctct cccaagtgtg aaacgcggtc
     7321 agctgttgcg catccgtggc ctttcggcgg tttctaattt aaatattgat attgcagaac
     7381 ggcaaaaacg gcgagcaatt caacgcctac accctcattt cgataatgta cggataaaaa
     7441 tcgaacaact gccctctcgt ttcaagggca cgatgttatt tttactcgca gaattccaat
     7501 ccgatgacac gcaaaatggc tacacgcaat gctgctatta ttctcttggt aaacttggca
     7561 agcctgccga ggccgttgct gacgaagcag ttgatgctct actagatttc ttgaatactg
     7621 atgctgccat cgaccaatat ctagcagatc agcttctgct accgttagct ttcgccacca
     7681 gccaatccgc aatccgcacg agtcaaatca ccccccacct gcttacaaat gccgaggtca
     7741 tacgccaatt tctttcggca aagatcgaaa tcgacagtga tatcggtaaa cctggattga
     7801 ttcgtgtgat acccaacccc tcatcttgaa accgccttga cgcgcaaaat cctccacctc
     7861 gacctagatg cctttttttg tgctgtcgaa gaacagcgcg acccgtcgct catcggtaaa
     7921 ccctttgcgg taggcgggcg tcctgagcat cgcggcgtgg ttgcctcttg ctcttatgct
     7981 gcccgaaagt atggtgtccg ttctgcaatg cccatgtccc gcgctttacg actctgccct
     8041 aatttaatca ttgtgtcttc acgtcacaga atttattctg acgtatcaaa acaagtgatg
     8101 gtacgtctgc gccaattaac cccgctggtg gaacaaattt ccatcgatga agcatatcta
     8161 gatgtcagcg aattaccaga ttcgggcatg gtcctcgccc agcgcttaca agcggatatc
     8221 aaagaacagc ttggattgcc ttgttctcta ggtgttgcaa ccaataagtt gctagcaaag
     8281 accgccaacg acgtaggaaa atctgctgca cgcagtacag gtccacccaa tgcaattacc
     8341 gttgtacctc ctggggatga agaggctttc ctctccccgt tacctgtcca ggcattatgg
     8401 ggtatcggac ccaagacagc tgaacgcttg aaagagattg gtgtcatcac gatcggagac
     8461 ttagcccgtc tccccgaaca agaattggtt tctatgttgg gaaaaaacgg ctatgatctg
     8521 gcaaagcgtg cgcgcggtat tgatgatcga ccaattatca ctgaacacaa gattaaatca
     8581 atcagtcagg aaacgacatt tgctcgagac attcgtgata agttcacatt gcgccaaaca
     8641 gttttagaat tatcagaaaa ggttggtcgc aggctaagag cctactcatt atctgccact
     8701 actgtaaagc tgaagattcg ctggcacgac tttactaccc taactcgcca aatgacgctc
     8761 agccacccca ccgatcagga ttccgagatc tctttgatcg ctctccagct tctggataat
     8821 gtttggaaac ccagccagct agtacgctta ataggcgtgg gggtcagcgg tctaaaacca
     8881 ccacaccacc aattgagctt ttgggatgcg tccgatcaaa aagacctatg aacctcatcc
     8941 agattaatac taaagacaag cgccaggtaa ataaattctt agaccttcca ttccgtctct
     9001 atgcatatgt cccccaatgg gtgccccgtc taatgcctga ggcacgccgc atgctggata
     9061 cgcagcgaaa tccatttttc aagcacggcg aagcagcctt tttcttggtg ctggacgatc
     9121 aagaaacccc tcaaggacgt ctggcgatac tcaacaacca caaatacaac gagtacaacc
     9181 aagagaaaac tgcttttttc tatctatttg aatgtgaaaa caaccagcaa atatccaatc
     9241 tgcttttcga agcagcattt gcctgggtgc gcttgcgcgg gttggataag attgtaggac
     9301 ccaaaggctt taccgttttc gatggtttgg gcatgctggt aaaaggtttt gaacaccgcc
     9361 ctgcgttcgg tttaccgtat aatatgccct attacccaga gctagttgaa gcagtaggtt
     9421 tccaatccaa aggcgaacta gtctctggtt atctaaactc gaaaatgcaa tttcctgcga
     9481 agattcacca ggtcgccgaa cttctgcaag aacgtcgagg tttgcgtgtg gcacgtttca
     9541 aaactcgccg tgatttgaga gcttttgtcc ctaaactaaa agctttgtac aatggcgcct
     9601 tggcaagcac agatggcaac gcgcccttat ccgatgagga agttaaagcc ctggccgatc
     9661 aaatgatatg gtttgccaat cctcgattat taaagatagt ctacaaggga gatcaaccag
     9721 ttggttttct gctcgcctat ccagatatat ccgcagctgt tcagcgtaca agggggcgaa
     9781 tcttcccttt aggttggatt gatcttctcc tcgagctacg acgcaccaaa tggattaaca
     9841 ttaacggagc aggaatgctt gaagggtatc ggggactagg cggcactgct ttgctcttca
     9901 gtgagatgca taaaagcgtc accgaagagg ggtatcagca tgccgacctg gttcaaatcg
     9961 gcacagaaaa cgaaaggatg ctacgagagc tacgaggtct gggcatcgat ttttacaaga
    10021 cacaccgcat gtattctcgt accatgtagg tcattgtatt catgttgcga cccgaaattg
    10081 actggggtat ttgccaaagt tgcctgcctt gtttagcagg ccacgcttgc cggactcgcg
    10141 ctattgtcca gattgatccg ggcgaggcgc catatataga gtattcgcgt tgtagcaact
    10201 gtgctttatg tgtcactgct tgttgctgtg aggcaatttt gatgaagaat ttaaccgcat
    10261 attcaggttc aatcattaaa taatttttcc agaatatcca ggaaactcat tcgatgacag
    10321 aactaaagtg gtggcagcgt gccgtcttct accagatcta cccacgtagc ttcgccgatg
    10381 gtaacggtga tggaattggt gatttccccg gcatgattca aaagcttgat tatcttcaag
    10441 acctaggtat cgatgccgtt tggctctccc cacattatcc atccccacag tttgattgcg
    10501 gttacgacat caccgattat aagggcatag cacccgagta tggcacactc tcagagttca
    10561 atcgctttat cgccggtgca caccagcgcg ggattcgtgt catcctcgat ctagttttga
    10621 accacacctc tcatgaacat ccctggttta ttgaatccag atccagccgg cacaatccaa
    10681 agcgcaattg gtatgtttgg aaagatggta aagtcgacga taacggcata ctccaacccc
    10741 caaataattg gaattcgact ttcgatggga ttgcttggga attcgaccct cccacaaatc
    10801 aatattatta tcactacttt ttaaagcagc aacccgacct caattggcac aaccccgaag
    10861 tcaagcgtga aatgtgggat tccgttcgtt tctggcttga tctgggggtg gatggattcc
    10921 gcttagatgc aatcagtacg atatatgaac gcccggagat gccaaaacat cccttagaca
    10981 tgagccttgc aagtttgcgc agaatcctga gagattcccg cacacccgaa gagcatgcac
    11041 aagcacgtcg tttacaaaag ctcatgttcc aatatcaaaa tcaccaacca ggcttgcatg
    11101 agctaatgca agagctgcgt tcggtcattg atgaatatga cgattgcgtc ctggttgccg
    11161 aggatgacga tatagcttac atgggaaatg gcgacaatga acttcatttg gtctttaact
    11221 tcccattgat gcgcacaggt cgattgaccc ctgcttgggt gcgtgccaat cagaaagaac
    11281 gcctgactac attagcctcg atatctccct cggcttggcc gtgcaacact ctgggcaatc
    11341 acgatagccc gcgcgtatat agtcacttcg gcgatggcaa aaatgaccaa caacttgccc
    11401 gtatatccct agcactcatg ctcacactcc gtgggacgcc atttctctat tatggcgaag
    11461 agataggcat gaccgacaat tatttagagc atatcgatga cttccgtgat cttcttggca
    11521 cctggcaata tcaggcagaa atcgatgaat ttggcacacc acccgatctc gccttgcagt
    11581 acgccgctaa actcactcgc gataagaacc gcactcccat gcagtgggaa aactcaccta
    11641 actcaggctt cagtcccgca aatgttcgaa cctggttgcc cgtgaacacc aattacgact
    11701 ctggcgtgaa tgtggctgat caggttgctg atccagcttc cctcttgaaa ttttatcaac
    11761 gtattctgag cttacgcaaa aataacccag cattgattgg gggcgactat cttcccctac
    11821 atgagcactc agatgactat ttggctttcc tccgcagggc agagcaacaa acctgcctgg
    11881 tcgtgttgaa tatggttgct aaacatctgc gtatccactt cgatttggaa ggggtatctg
    11941 cgcaattgct cttttcaagc caggaacgcc aaacaaattg gctcgatctt aatttaatcc
    12001 agctttcccc cttcgaaatt ctaatagccg aaatgatata aaagcaggga accgattcgt
    12061 acacaatcga ttgtgcacgc accttttccc tatcaatgtt gttagaaaag taaaatactt
    12121 acacaatatt cattgacaca ctcccgcatt aaaggtattt taacaacaca gcgaactttg
    12181 atcccatatg tcttcgattt ccaggagata gcaacaaccc tctcatgggg gtgacaatcc
    12241 tacattgagc ataatctatg gacaaagaga ctattctcgt cgttgacgac aatcaccaaa
    12301 tatcaagatt tctagcacat gagttattac cttccttggg ctattcggcc atggtcgccc
    12361 gcaatgggaa atcagcttta gaggccttgc gcggggagaa agtagctctc atgctattgg
    12421 atcttcagct acccgatatg agtggtttag atgtcttgaa acaggtggct aaagagggtc
    12481 taaacgtccc aacaatattg gcaactgcac atgggtcaga aaatatagcc atcgaatcct
    12541 tccgtttagg agtcgaagat tatctcatca aacccatcga ccccgatcga ttgaatgatg
    12601 ccatcacgca tgcactctcc gaatcgcggt tgcggcgcga gaaagcagtc ctgaccaaca
    12661 agttgcgaga gcagctttct tggcaatcag tactgttcaa gattggacag agcgttattt
    12721 cttcactgga agccgatgaa gttctaagac gcatcgttga ggctggcgtg cttttaactg
    12781 gcgccgagga gggattcctg gctctcctgg acgaacagac agaccggcta tttctgcgtg
    12841 cctcaaaaaa tattgaccaa gaaaagagca agacaatgcg cttgccgatt actgataatc
    12901 tgatcgccaa agtgctgcag accagaaagc ctgttcgcac catcacgcaa actgctgaag
    12961 accctttgat caaattgagt acaggatttc tcgtccgcag tctactccat gtacccatta
    13021 tttcacgcgg aaaagcgctg ggtgtcctta caatggttaa tcacagccat caaaaaccat
    13081 ttctggataa ggatgaatcc ctaatgacat ctctggcgag ttatgcctca attgcgttgg
    13141 acaacgccgg tttgtatcat cgagcgcaac aagagatcat tgagcgacga aaaatagaag
    13201 aagcattgcg cgagagcgag gaacgatacg cgctggcgat gcgtggttca aatgatggct
    13261 tgtgggattg ggatctccgt tcgaatgaga tatatttctc accgcgttgg aaatctatgc
    13321 tggggtacag tgaaaaagag atcactaata gccctcaaga atggttttct cgtgttcatc
    13381 ccgacgatat cgataaacta cgcatagata tcgaaaccca cttaactaga aattcacctc
    13441 attttgaaaa tgagcaccga ctacaacaca aagatgggga ttatcgctat atacttagcc
    13501 gtgggatagc cgtttcagac gccaatggga tggctcagcg tattgccgga tcaatcacag
    13561 atataaccga aagaaaagct gtcgaacaga aacttctcca gtatgccttt tatgataagc
    13621 tcacaggact tccaaatcga gcgttattcg ttgaccacct gagtttggca atcgagcggg
    13681 ctaagcgacg gcctgatttt caatttgcag ttctgttcat ggatttagac acatttaaag
    13741 acgtaaacga tagcctgggg cacatggtcg gcgatgaact acttgtctct gttgcacgct
    13801 tattgcaatc acgtatgcgt tccacagaca ctgttgctcg tttcggtggg gatgaatttg
    13861 ttattctgct tgacgatatc cacgataaat tcaacgccca acaagtgtcc gagtggattc
    13921 agtcagccct cgctgagcca ttcatcttga atggacagga ggtttacatc tccgccagta
    13981 ttgggattgt actcagcgaa acaggttacc agcgctccga agaagtcttg cgcgatgccg
    14041 atatcgccat gtacaacgcc aaagccaacg gcaaatcaag gtttgagatt ttcaaccccg
    14101 aaatgcgcac tcgtttattg gatcggttgg aactagccaa tgatttgcga agggcaattg
    14161 agaatcaaga attgcgcgtc cattatcagc tcatcgtttc cttgaataac ggtcgaatca
    14221 caggtctcga agcattggtt cgttggcagc acccgcagcg tggcttgctt gctccaatcg
    14281 aatttattcc attagctgaa gatactggct tgattatcgc cctcgaccga tgggtcttgc
    14341 gtgaggcttg ccgccagatg cactcttgga aacttgaaaa ccccatgttt tctgatctaa
    14401 ccattagcgt gaacatctct ggcaagcagc tatctcaagg ggacttcatt agctttgttg
    14461 aacatacact cgaagcagct gaccttgatc caaaatatct ctgcttggaa atcacggaaa
    14521 gtgtcgtgat cggtaacaaa gagatcacgg tagaaatgtg caataaattg aggcagcttg
    14581 gtgtccatat ccatattgac gattttggaa ttggttactc atcacttagt tacctgtctg
    14641 attttccagt caacgctcta aaaatagacc gtagcttcat tagcaaaatg agcgagggaa
    14701 agaacgaagc ggacattgtc caagcaatcg tcacgctctc acaacgaatt ggtgtcgatg
    14761 tgattgctga gggggttgaa acgagtggac aactcaagaa attgcaggct atgggctgtg
    14821 aatatggaca aggtttttat gtctccaaac cgctagctgg agacgaggca aagaaattgg
    14881 tccttcaatc gcttgttgtg agcgtttccc ctagctaata ctgcaggaat agcgcatttg
    14941 aatgaaaaat cctcacctgc gcaacttctg actgtcaagt ttctatattt ctgttagaat
    15001 ttagacataa atctttgctt gttaccgttt ttcggtaaac gaaatgttta tcgatcattt
    15061 aacgttatat atctggatgc gtgatgaaac cagcaaaact accaattcaa tatcatcaag
    15121 tctctcccag ccagtgggat aaggtagaca cagaagtaat cgtcgaaact ccagtttccc
    15181 tgacagtaaa tggggaagcc tggcttacat tcatgtgtac cccggtagat ttagaggcaa
    15241 tggcggtcgg attcttgttc aatgaaggat tgatcaaatc cgctgacgag gttgccgacg
    15301 tccgtctttg tgatactggg gataacttag acgtctggct ccatcgttcc gtagataagc
    15361 ctgacaaatg gagtcgcaca tccggttgca ccggtgggat gacttcggtt gagagcaata
    15421 tcacatctcc agccattacc aacgggggag tcctttacgc ggatcgagtg gggcaactga
    15481 tcgcccaatt gttcgaatcg cagcagttgt accgcgcttc tggtggagta cacacttccg
    15541 ccctcagcga tgggcagcgc atctggataa tcgctgaaga tatcggtcgg cacaatactc
    15601 tcgacaagat cgctggtcat tatcttttgg aaggtctgca accccagaaa cgaatcatcc
    15661 ttaccaccgg tcgcatcagt tcagagatgc tgcaaaagtc cgctcgatta aattcttctg
    15721 ttgtgatttc ccgcacatca cctagctccc tatccatcga gctagcccaa aaatggggaa
    15781 taactttgat cggatacgcc cggagaaata aattcaatat ttatacacat ccggaacgaa
    15841 ttgtgttcgc tccggaaggc aaaaaagcca aagaagagcc cattttcgta tgaaaatggg
    15901 ctcttttatc ataataagcg agagggagca atgaccaatc atcacatccg ctttaaacca
    15961 cctgatccgc aaatcgctgc cctagtcatg gctcacgctg gaaaacccga agctgtcttg
    16021 gaggtgttga aaggtgttca agatctatat cgcgggttgt cccaagagaa cattcaagat
    16081 gtcgccctca gcctaaatat tccagccgag agagcttatg gtgttgcatc tttttattcc
    16141 atgctctcat tagagccagt cgaaggaaag attatccgtg tatgtgacgg tccggtttgc
    16201 tggctaaaac gagcatccga ggttcagtta gctctcgaga gcgaggctgg aagggcaggc
    16261 tacacgatcg agcgcagcag ttgcctgggg ctatgtgacc gcgctcctgc tgctttggta
    16321 gtgcaagagc aggcaggacc agttaatcca ggtgatgcgc gcaaactggt gcaaggcgag
    16381 cgaggtgtga gtatagacta tagccatcct cggcgtggag aagtacgcgt gatgatggca
    16441 caggctggca tggttgaccc agattcgctc caatctgccc taagccatgg tgtatatgag
    16501 ggcctgaaga aggctttgag tctcactcct gagcaagtag tagctgtagt tgaaaattct
    16561 ggcctgaccg gacgtggagg ggctggcttc cctgttggac gtaaatggcg cttcgtcgcc
    16621 caatcaggtc ggatgcctag atacgtcata tgcaacgctg acgaatctga gcctctgatc
    16681 ttcaaagata gggttttgat cgatacgaac ccacaccaga tcttggaggg catggcaatc
    16741 tcaggctacg ccacagggtc gcacgaagcg ttcatctata ttcgtggcga gtatgcagct
    16801 caagctgctc gcctggaacg cgccatcagc caggccgaag aagctaatct gcttggggaa
    16861 agcatcctgg acagcgggtt ctcatttcac atccatgttc acatgggggc tggagcgtat
    16921 atttgcggcg aagagacagc tctcatcgaa tcgctggaag gcaagcgggg tgagccacgc
    16981 gctcgcccac cctatccacc ttcttatggc tacatggggc agcccaccct tgtaaataac
    17041 gtggagtcat atgctgctgt gcctgccata ctacaaaatg gcgcacaatg gtatcgctcg
    17101 attggtaaca ataacggcgg cacatcccag cctgggataa ccccaggcac aaaattgtac
    17161 atgatcttgg gccatattaa tcaaccaggg ctattcgaag ccccgtttgg cttgacttta
    17221 cgccaagtaa ttgacgaatt cgggggcggt ctgcttccgg gatcgcagtt caatttcgcc
    17281 cttactggtg gcgcggcagg cactatcgtc ccgccctcac tgcttgatgt gtccatcgat
    17341 tatgcttcgg cggccatggg tgtctcgcta ggtgctggag cgttcctgat ttgcgatcac
    17401 acggtatcgc ctatcgcctt tctaagggaa ttactccact tcttcacggt ggaatcgtgc
    17461 ggaaaatgca ctccgtgccg tatagggacc cagcaatctc tgctaatctt aaatcgtatg
    17521 acccttggtg aaggcaaaac cggggatgtt cagcaactta gacaactagc tgacttaatg
    17581 caacaagctt cattctgcgg tctaggacag tcggttgcaa ttccagtcaa atcagcgctg
    17641 actcactttg ccaaacagtt ccagagctct gaacaggcac agtaatattt tgggagatga
    17701 caatatggcg attaacgtca caatcaatga gcaaaccatc aacgccaaag aaggtcaaac
    17761 cattctggag gccgctcaag atcacggaat cactattcca accttgtgct atcacaaaga
    17821 tctctctcca gtgggctcat gtcgcctatg cctagtagaa gtagacaaat ggcgcggcca
    17881 gattgccgct tgcaccaccc aggtagctca agatatgatt gtgcgtacag aaacacctgc
    17941 gctgatccaa tcccgacgcc taattctgga aatgcttttg caaaattatg ttgatgcggg
    18001 ctatgcttca ggtgatgaga aacacgatga atttatggct tgggtgcggc actaccaggt
    18061 tgcgctaccc gaggggagta ttcctgccca gcgctaccga gtagacagcg atcccaaccc
    18121 gttcttatgg gtagatctca ataaatgcat cctatgcacg cgctgcgtgc gcgcctgcgc
    18181 cgagattcag gggcgttttg tctggggggt gggtcagcga ggatatgagg ctcacatcat
    18241 tgctggcgca aataccgatc tgctcaacgc acgctgcgag tcttgcggcg cttgcgtaac
    18301 gtattgccct accggcgcgc tcgatcataa gatgtctatc aatttaggtc atccagataa
    18361 agtagtcacc accacctgca cctactgcgg ggtaggctgc caactcgacc tgaatgtcaa
    18421 ggataaccat gtcattcggg tgacatctaa cccgaacgca ccggtcaacg ggaatcactt
    18481 gtgtgtcaaa gggcgctatg gatacgattt tgtccatcac ccggaccgtc ttacccaacc
    18541 aagggtgcgt agctatttac tgcaggatga tagcaaacct aaaggagatt taggggagtg
    18601 ggtcgaagtc gattgggaca cagccctgga aatcaccgcc cgcaaattaa cccatacccg
    18661 agatacattt ggcccggata gtatcggtgt cctgacctcg gcaaaatgcc tgaatgaaga
    18721 aaattatttg atgaataaat ttacccggca ggtgataggg actaataata tagatcactg
    18781 cgcccgtctc tgacactcga gcactgtggc cggtctggcc acctcatacg gctcgggcgc
    18841 catgtccaac tcaatggatg atatcgccaa tcatgcccaa tccatgctgg tgatcggctc
    18901 caacacgacc gagcaacatc cggtgtttgg cagcatgatc cgccaggcga ttctcaagcg
    18961 caaggccaaa ctggtcgttg cggaccctcg caaaatcgat ctcac
//
LOCUS       DBNGSX010000007         5164 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_277295, whole genome shotgun sequence.
ACCESSION   DBNGSX010000007 DBNGSX010000000
VERSION     DBNGSX010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_277295"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="AC16CB_00230"
     CDS             <1..308
                     /locus_tag="AC16CB_00230"
                     /inference="COORDINATES: protein motif:HMM:NF014667.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR3340918.1"
                     /translation="KVRFIFITVDPVRDTVEQIGSHLGNFDPALIGLTGSRAELEAVW
                     KNYGVFQSQHEGEGVEHYDVDHSSRIYAIDPQGNWRLTYPAEMDYETIAKDLEHLID"
     gene            308..832
                     /locus_tag="AC16CB_00235"
     CDS             308..832
                     /locus_tag="AC16CB_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR3340919.1"
                     /translation="MNAKFDVGPYIIRFIISALISVVVAGLINEGAYLFQKEKYDRPP
                     KTIQLVVPNGTADQLAAGESIKSIPENMVFVAGDVLEVKNEDSVAHQLGPVWVPAGAT
                     GRLDLNDPNKYAYSCSFTTQRYLGLDVRQPTTLGTRLTALAVGGPPLLVFFFIYSLLV
                     FPVKPAQKEAVGQA"
     gene            832..1626
                     /locus_tag="AC16CB_00240"
     CDS             832..1626
                     /locus_tag="AC16CB_00240"
                     /inference="COORDINATES: protein motif:HMM:NF014193.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial respiratory chain
                     complex IV assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HIR3340920.1"
                     /translation="MSGHAGSLVVHKRGGIRALRVFSRRWILSTLLVLAAAAVLARLG
                     LWQLDRLEQRRAFNARVQAQISQPVLELTAGTMGLDLASMEYRAVHLRGEYDFSHQVA
                     LRNQVWGNEPGVRLLTPLKIEGTDSYILIERGWVPGLESTPQDWQQYDETGMVEINGM
                     IRASQSKPDFGRRADPVPAPGEESLRLWHFANVERIADQVPYRLLAVYIQQAPSLALT
                     GLPNRSLPELELTEGPHMGYALQWFSFAVLLLIGYPYYVNKRELKR"
     gene            1678..2298
                     /locus_tag="AC16CB_00245"
     CDS             1678..2298
                     /locus_tag="AC16CB_00245"
                     /EC_number="1.17.99.9"
                     /inference="COORDINATES: protein motif:HMM:NF014665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HIR3340921.1"
                     /translation="MLQKFSRYPYIVLTALLGVFILIVMGGVLSVTGAGGACPDWPTC
                     FGAWTPPPASLEQPLADYLHRFGTALATFFILAAVWRGKRSQPWIIRPLLAALGTLLV
                     QVLLGAWMVLNPQAAGRAWISALHLGLSLIMLAALAVATVVAFVIRGRPSKDNEHRLV
                     LSFASPFARLSLVTLVATFLLLVTGAAVAAGEATQACLGWPLCNGM"
     gene            2323..3492
                     /locus_tag="AC16CB_00250"
     CDS             2323..3492
                     /locus_tag="AC16CB_00250"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HIR3340922.1"
                     /translation="MNLSHRLVVGLAGILVAGLVWRAWLTQRTQTPVLVSATAVGALF
                     FAQALMGAKMVQGFPPYLLVLHQATAVATWAALVVQVVTVGLAGRSSEQERVEAIAGR
                     GSRWQVRDYLMLTKPIVVALLLVTTYAGMVIGAKAWPSFWLTFWTLLGGFMAAGGSGA
                     INQYIDRADDLKMQRTQKRPIPSGRLTPGQGLAFGVGLALVSFYLMAALVNFTAALLT
                     LAGIIYYVLIYSLLLKKTTVQNIVIGGGAGAIPPLVGWAAATGGLNIPSLFLFAVVFM
                     WTPPHFWALALVRRNDYARAGVPMLPVVRGERETRWQIFIYTLELVALTLLLPLFGLG
                     GSVYLVGALALGGWLLLTAWKVWKQGGNRVAWKMYRYSSMYLAFLFVVLMVDALI"
     gene            3749..4243
                     /locus_tag="AC16CB_00255"
     CDS             3749..4243
                     /locus_tag="AC16CB_00255"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3340923.1"
                     /translation="MEVQKSILITASPEAVWPFMVQPDKVLQWYLTFQKFEYTSKPRS
                     GAGTTMYIEEKAAGPLMKMNFIVTEWVANQLLAFRMEPGAGPKSYEQRWSIEPSPSGC
                     IVTLWEEVQLPYGVIGKWVERIAQPKSEATVEKILVKLKTLVEAVEEETPETISDAHL
                     ELQF"
     gene            4566..4949
                     /locus_tag="AC16CB_00260"
     CDS             4566..4949
                     /locus_tag="AC16CB_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3340924.1"
                     /translation="MSPIRMSKVESGIRVVLEFNEAFNRHDVAGMMRLMSDTCVFENT
                     HPAPDGTVYTGKEVVTQYWQEFFHQSPHAHIAIEEIFGLGERCIMRWRYSWVDLEGEK
                     GHVRGVDIFLVRDGGIKEKLSYVKG"
BASE COUNT         1083 a         1255 c         1515 g         1311 t
ORIGIN      
        1 acaaagtgcg ctttatcttt atcaccgtag atcctgtacg tgatacggta gagcagatcg
       61 gctcgcacct ggggaatttt gacccggcgc ttatcggact tacaggcagc cgggcagaac
      121 tcgaggcggt ctggaagaat tacggcgtgt tccaatccca acatgaaggc gaaggcgtgg
      181 agcattacga tgtggatcac tcgtcgcgca tctatgccat cgacccacaa ggcaattggc
      241 gattgacgta tccggctgag atggattatg aaaccatcgc caaggatttg gagcatttga
      301 tcgattgatg aacgcaaagt ttgatgttgg tccctatatc atccgcttta ttatctccgc
      361 gctgatctcg gtggtagtgg ctgggctcat taatgaggga gcttacttgt tccagaaaga
      421 gaaatatgac cggccgccaa agaccatcca gctcgtcgtc cccaatggga cggcagacca
      481 gttggctgcg ggagagtcca tcaaatcgat cccggagaat atggtctttg tggcgggtga
      541 tgtgctggag gtcaagaatg aagatagcgt agcgcaccag cttgggcctg tctgggtgcc
      601 agccggcgct accgggcgct tggatctgaa cgatcccaat aagtacgctt attcctgttc
      661 ctttaccacg caacggtatc tggggttgga cgtgcgacag ccaaccaccc tggggacacg
      721 cctgacagcc ttggcggtgg gtggtcctcc cttattggtg ttctttttca tctatagtct
      781 gctggtattt ccagtcaagc cagctcagaa agaggcagtg gggcaggcgt gatgagcggg
      841 catgctggca gtctggtggt gcataagagg ggtggtattc gcgccttgcg cgtattcagt
      901 cgccgctgga ttctttcaac gctgctggtt ttggccgcgg cggcggtgct ggctcgcctg
      961 ggcctctggc agcttgaccg tctggagcag cgccgcgcat tcaacgcccg tgttcaggca
     1021 caaataagcc agccggtatt ggaattgacc gctggcacaa tggggctgga tctggcaagt
     1081 atggaatacc gagctgtaca cctgcgcggc gagtacgatt tctctcacca ggtagctttg
     1141 cgaaaccagg tgtggggcaa tgagcctggt gtacgcctgc tcaccccatt aaaaattgag
     1201 ggcactgata gctatattct catcgaacgc ggctgggtgc ctgggcttga atcaaccccc
     1261 caggattggc agcagtatga tgaaactggc atggtcgaaa ttaacgggat gatccgcgct
     1321 tcgcaaagca agccggactt cggtcgccgc gctgatcccg taccggcacc cggtgaggag
     1381 tccctacgcc tgtggcattt cgccaacgtg gagcgtatcg ccgatcaggt gccctatcgg
     1441 ctgctggcgg tttatatcca gcaggcgcct tctctagcct tgacgggtct gccgaatcgc
     1501 agcctgccag agctggaatt gacggaaggg ccgcatatgg gttacgcatt gcaatggttc
     1561 agctttgctg tcttgttgtt gattgggtat ccatattacg tgaataaaag ggagcttaag
     1621 cgctgacctg ctcccgggta ttccttgatt aaccgagcgc gttgcacaca aggtattatg
     1681 cttcaaaaat tctctcgcta cccatatatt gtcctgaccg ccttgcttgg cgtctttatc
     1741 ctgattgtca tgggaggcgt gctgagcgtc acgggcgctg ggggcgcctg cccggactgg
     1801 cccacctgtt ttggggcgtg gacacctccc cctgcctcgt tagaacaacc gttggcggat
     1861 tatctgcacc gtttcgggac ggctctggct acgttcttca tcctggcggc agtctggcga
     1921 ggtaagcgca gccagccctg gatcatccgt cccttgctcg cggcgttagg gaccttgctc
     1981 gttcaggtgc tcttgggagc ctggatggta ctgaatccgc aggctgccgg tcgggcttgg
     2041 atttcagcct tgcacctggg tttgtctttg atcatgctgg cagcgctggc agtggctacg
     2101 gttgtagctt tcgtgataag aggacgtccc tcgaaagata atgagcaccg cttggtattg
     2161 agcttcgctt caccctttgc tagactctct ctggtaaccc tggtggcgac cttcttgctc
     2221 ttggtaactg gggcagctgt tgcagctggt gaggcgacgc aggcttgtct cggctggcct
     2281 ttgtgcaatg ggatgtaatt accagataac ctggttggtt gggtgaacct gagccatcgg
     2341 ctggttgttg ggcttgcggg catcctggtt gcaggccttg tttggcgcgc atggcttacc
     2401 cagcgtaccc agacgcctgt gctggtctcg gcaacggcgg taggggcgct cttcttcgcc
     2461 caggcgctga tgggagccaa gatggtgcag ggctttccgc catacttatt ggtcttgcat
     2521 caggcgactg ctgtcgcaac ttgggcggcg ctggtggtgc aggtggttac cgtcggcttg
     2581 gctgggcgct cgtcagagca agaacgggtt gaggcgattg ccgggcgcgg ttcaagatgg
     2641 caggtgcgcg attacctcat gctcaccaag ccgattgtcg tggctttgct gctggtgacc
     2701 acctacgccg gcatggtgat tggggcgaaa gcctggcctt cattttggct cacattttgg
     2761 acgttactgg gcggtttcat ggccgcgggc ggctctgggg cgattaacca gtacattgat
     2821 cgcgccgatg atttgaagat gcagcgcacg cagaagcgtc ccattccatc aggcaggttg
     2881 acccctgggc aaggattggc ttttggcgtt ggcctggcgc tggtgtcttt ctacctgatg
     2941 gctgccctgg tcaatttcac agctgccttg ctcaccctgg ctggaattat ctattatgtg
     3001 cttatctaca gcttgctgtt gaagaagacc accgtgcaga acattgtcat cggcggaggg
     3061 gcaggggcta tcccgccgct ggtgggctgg gctgcagcga ctggtgggtt gaatatcccc
     3121 tcacttttct tgttcgccgt ggtcttcatg tggacgccgc cgcacttctg ggcgctggcg
     3181 ctggtgcgtc gcaatgatta tgcccgcgcc ggggtgccca tgctgcccgt ggtgcgcggt
     3241 gaacgcgaaa cgcgctggca gatctttatt tatactctgg aattggtggc gctcactttg
     3301 ttgctgccgc tctttggact gggtggcagc gtctacctgg tcggggcgct ggcgctgggt
     3361 ggctggctgt tgctgactgc ctggaaggtg tggaaacaag gaggtaaccg ggtagcctgg
     3421 aagatgtacc gctattccag catgtacctg gcgttcctct ttgtggtctt aatggtagac
     3481 gcattgattt aacggtatct tttatcggta tagaggacat ggcctggaag caaccttcca
     3541 ggccatgtcc ttattaattg acgcactata cttgacagca gatataattt gtaattgatt
     3601 cttgtgcagt aataatgtat actggactca agtctcaccg ttacggaatg catcgcaggc
     3661 gggagcctgt tatgggctta attcaggatc atgttcgttt agaccgctag caagagcttg
     3721 gcggtttttg ttgtgaaagg gaactacgat ggaagtacaa aaatcgattc taattacagc
     3781 ctcaccagaa gcagtttggc cttttatggt tcaaccagat aaggtcctcc aatggtatct
     3841 cacgttccaa aaatttgaat atacaagcaa acctcggagc ggtgcgggta caacgatgta
     3901 catcgaagaa aaagcggctg gtccactgat gaaaatgaat tttatcgtca cggaatgggt
     3961 tgctaaccaa ttgctggctt ttcggatgga gcccggcgct ggaccgaaat cctatgagca
     4021 gaggtggagc atagaacctt ctccctctgg atgcatagta accttatggg aagaggtcca
     4081 actgccatat ggggtcatcg gtaaatgggt agaacggatt gctcagccaa aatccgaagc
     4141 cacggttgag aagatacttg taaaactgaa gacacttgtc gaagccgtag aagaagagac
     4201 tccagagaca atatccgatg cacacctcga actgcagttt tgattgagag aaccgtggat
     4261 agcattaggg gctgacccaa tattgtcttt gcaaaagccg ctgacgagat tcggcggctt
     4321 tccatttggg taaaaatata actgtgatac cgatcttaac tactgataac tattgtgcat
     4381 aatatgtagc aaccacttaa ttgtttgtcg gttaaaatca ttaatgtgaa gaattcaaga
     4441 ggttgtcaca atatgcgctg aataaatccc ttggctgtcc ggcgtgagga gaaatgttca
     4501 ctaagtagca aatcctatgc ggtcaggcag ccattcggat ttctcctgaa ggaggtatga
     4561 accaaatgag cccaattcgg atgtccaagg tggaatcagg aatacgcgtc gtccttgagt
     4621 tcaatgaggc cttcaaccgt catgatgttg ccggtatgat gcggcttatg agcgacactt
     4681 gtgtcttcga aaacacgcat ccagcccccg acggcactgt atacacgggc aaggaagtgg
     4741 tcactcaata ttggcaggaa ttcttccacc agtcgccgca cgcccatatc gcaatcgagg
     4801 agatttttgg tcttggtgag cggtgtatca tgcgctggag atacagctgg gtggacttag
     4861 aaggtgaaaa agggcatgtc cgaggcgtcg atatattcct ggtgagagac ggtggcatca
     4921 aagagaagtt gtcctacgtc aagggataga ttggatttga gtggaggata tggttctgcg
     4981 gattgtgctc gaagataacc gtctccatcg attgctgttt atctgtctat ccgcctccac
     5041 ctttcttgca ccgagctatt cgccaatttt catgctacga tgaagcggta cgctggctga
     5101 atccaagttt gggtgagcga cattgatgag acaaacgcca gtgaaaactt gatcacaggc
     5161 gatg
//
LOCUS       DBNGSX010000008         5943 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_554327, whole genome shotgun sequence.
ACCESSION   DBNGSX010000008 DBNGSX010000000
VERSION     DBNGSX010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5943)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_554327"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..871)
                     /locus_tag="AC16CB_00265"
     CDS             complement(65..871)
                     /locus_tag="AC16CB_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340925.1"
                     /translation="MTTSKRLTGLLMVIVAAGSLMITLAALVQVWRLRQPVTQYLRSN
                     LRVVHESLGTTSDGLLLTSQSLDSVQASVTALSVTVDTLGKSIQDSEPMMDSLVVLMG
                     EDLPETISSAQTSLSSAQESAKIIDAVLRALTIFNPRIYNPRVPLNDALGQVSESLDD
                     LPKSFSNMEVGLKSTQRNLTTIQVQIDLMSGEIERINESLDGSRKVINQYQGLFTRAE
                     KRVINIKNRAPATVTTLAWVLSFLLFWLGVAQIGLLIRGLEWLRVKKQPD"
     gene            999..1766
                     /gene="surE"
                     /locus_tag="AC16CB_00270"
     CDS             999..1766
                     /gene="surE"
                     /locus_tag="AC16CB_00270"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIR3340926.1"
                     /translation="MHILVTNDDGVTASGLLALAQEMRQVGQVTVLAPDRNWSASGHV
                     KTLERPLRVRDVILADGTPALATDGAPSDCVALAVLGLLPSKIDLVVSGINPYANLGH
                     DVTYSGTVTAAMEAAIWGLPGIAVSLNSTENDRGPLDYGPAARVARRVVAEVIARQLP
                     KATLLNVNVPYLPDEQLKGIRITRQGLRVYHDLLVTREDPRGRPYYWIGGDAPTGVPE
                     NGTDFGALSEGFVSITPLHLDLTAYRALEEMSEWKWE"
     gene            1866..2141
                     /gene="rpsT"
                     /locus_tag="AC16CB_00275"
     CDS             1866..2141
                     /gene="rpsT"
                     /locus_tag="AC16CB_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR3340927.1"
                     /translation="MANIKSAIKRNKQNEKRRLRNRIFRGRARSAVKRARLAIDANEV
                     DSARQAALEAISALDKAAEKGVLHKNNAARRKSRLMRHLATLEKQSD"
     gene            2364..4172
                     /gene="argS"
                     /locus_tag="AC16CB_00280"
     CDS             2364..4172
                     /gene="argS"
                     /locus_tag="AC16CB_00280"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIR3340928.1"
                     /translation="MFEAEQQAIEKSIIAYCAQSGLPEPGPLQWSPIPFSGEWGISTS
                     FFQLAAQEARQGKSGGVPVPQRAAEIAAGVAAYLGTPPGYSRVEAVKAYLNCYFSASE
                     YARRVVDTVLTQGADFGRGAVKGERVMVEYANTNTHHSFHIGHARNALLGEALARLVE
                     FAGFETIRASYPGDIGLGVITVVWAYQKFYLGQEPSGVHERGQWLLKIYVEATKLLEP
                     REDETPEERAQRESYETERREIYRRWDANDPRVRELWRMTRQWSLDEQLAILDMLDIH
                     IDVWFFESEVDESAKTIVDELVALGVADDERSNGGPVIVKIDEKLGLKKEKYRSNIIL
                     RSDGTTLYLTKDLALAKEKFEKHHVDRSIYVIDVRQSLHMQQTFKILELWGFKQAEKC
                     FHLGYGFVSLPEGAISARRGRIVLFKDVADEAIRRVLSVIAEKNPEMPESDRQVVARQ
                     VGLGALAYSMLSVDTNKDIVFDWDAALNFDGHTAPYIQNAYVRANSILKKAGGLPTPA
                     SFDMPFSSHEVQLIDLISRFPQVVQSAAGEYRPLVMANYAYDLASTFHSFYHVVPVIQ
                     ADTEAMRDARLRLVAAARQTLANALRLLVIQPPEVM"
     gene            4249..5568
                     /locus_tag="AC16CB_00285"
     CDS             4249..5568
                     /locus_tag="AC16CB_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HIR3340929.1"
                     /translation="MSPTRILIMGAAGRDFHNFNVFYRDNPDYEVVAFTATQIPDIEG
                     RLYPPELSGPHYPKGIPIFSENELVKLIREMQVDMVDFAYSDVPHEYVMHKASLALSA
                     GADFHLLGMGSTQLKSSKPVISVCAVRTGSGKSQTTRRVSLILRGMGYKVAVIRHPMP
                     YGDLTKQVVQRFADYGDLDKYNCTIEEREEYEPHLDNGMIVFAGVDYERILRQAEQEV
                     DIILWDGGNNDFPFYVSDLKIVVADPHRPGHELSYHPGETNARSADVFVINKVDTAPP
                     ENVIKVREHLRSLNPNAVMIEGASPLFVDDPGAIQGKRVLAVEDGPTLTHGEMAYGAA
                     WVAARRFGAAEIVDPRPFAVGSIKATFEKYPTIGAVLPAMGYGGQQIKELEETINRSN
                     ADLVLIGTPIDLNRLVKINKPNQRVRYELQEIGQPTLQDLLKAKFGK"
     gene            5628..>5943
                     /locus_tag="AC16CB_00290"
     CDS             5628..>5943
                     /locus_tag="AC16CB_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013987847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HIR3340930.1"
                     /translation="MENTLALTITHRYWPRANGLLRDILLIGAGSFLVAALAQVRIPL
                     PFTPVPLTGQTFAVLLVGAALGSRRGAASLALYLSAGLLGLPVFTGLGSGLAHFAGPT
                     GGY"
BASE COUNT         1331 a         1583 c         1629 g         1400 t
ORIGIN      
        1 tcaggctcaa aacgatacag agaaatgatt gatatggggg cccttgcccc catatcaatc
       61 atcattaatc aggttgcttt ttaactctca gccattccag tccgcgaatt agtaatccta
      121 tttgagcgac acctagccaa aagagtaaga agcttaacac ccaggccaat gtggtgactg
      181 tggctggagc gcggtttttt atgttgataa ccctcttttc cgctctggtg aacagcccct
      241 ggtattggtt aatcactttt ctcgatccat ccaggctttc gttgatgcgc tcgatttccc
      301 cagacatcaa gtcgatctgc acctgaatgg ttgtcaggtt acgctgcgtg cttttcagcc
      361 ccacctccat attggaaaaa gattttggca ggtcatccag gctctcagaa acctggccca
      421 aggcgtcatt cagcggaacg cgtgggttat agatcctggg gttgaaaatc gtcaatgcac
      481 gcaggactgc gtcaatgatt tttgcgctct cctgagccga gctcaaagat gtctgggcgg
      541 aggagattgt ttctggcaag tcctcgccca tcaagaccac cagcgaatcc atcatgggct
      601 ctgaatcctg gatggatttg cccaaggtgt ccacagtgac actcaaggca gttacactgg
      661 cctgcacaga atctagcgac tggctggtga ggagcaggcc atccgaggtg gtccccaggc
      721 tttcgtggac cactcgcagg ttggatcgaa gatattgcgt caccggctgg cgcagtcgcc
      781 aaacctggac caaagccgcc aaggtgatca tgaggctacc ggctgccaca ataaccatca
      841 gcagaccagt gaggcgctta gaggttgtca tctttttccc ttatggctag tatgcaagga
      901 tcagaaccca ttttaccatc tcaatctttc atcttgtgtc atttgattta taattcatct
      961 ccatcctatg agtttcacct accgatagat aaaaacctat gcacattctt gtcacaaacg
     1021 atgatggtgt aactgcctca ggcttacttg ccttggcgca ggagatgcgc caggtgggtc
     1081 aggtgaccgt cctggcacca gaccgtaact ggtcagcctc tgggcatgtc aaaacgctgg
     1141 aacgtccctt acgggttaga gatgtcatct tagccgatgg taccccggca ctagccaccg
     1201 atggcgcacc atcggattgc gttgcactgg cagtgttggg cttgttgccc agcaagatcg
     1261 atctggttgt gtctggtata aacccctatg ccaacctggg tcacgatgtg acctactcgg
     1321 gaacggtcac tgccgccatg gaggcagcca tctgggggct tcctggaatt gccgtttcat
     1381 taaattccac ggagaatgat cgcggtccgc tggattacgg tcctgcggcg cgcgttgccc
     1441 gtagggtggt ggctgaggta atcgctcgcc aattgcctaa agctacttta ttaaatgtaa
     1501 atgtccctta cctgcctgat gaacagttga aggggatacg gatcacccgg caggggctgc
     1561 gcgtatacca tgatctgctg gtgacccgcg aagacccgcg cggccgcccg tattactgga
     1621 tcggcggcga tgcccccacc ggtgtcccgg aaaatgggac tgatttcggt gcactgtcag
     1681 aggggtttgt ctcgatcacc ccactgcatc tggacctgac cgcctaccgg gcgctggaag
     1741 agatgagcga gtggaagtgg gagtgacctt ctgaataccg gcaaatcgag ttcagaactg
     1801 gacaaatccg ccatcctcat gtaaaataac acgccgtact ttagttcgat gttggaggca
     1861 tatctttggc taatattaaa tctgcaatta agcgcaacaa acaaaacgaa aagcgccgct
     1921 tgagaaaccg tatcttcaga ggccgcgctc gctcagctgt gaagagagcc cgcttggcga
     1981 tcgatgcaaa cgaggtggat tccgcccgcc aggctgccct ggaggccatc agcgccctgg
     2041 acaaggctgc cgagaagggt gttctgcata agaacaacgc tgctcggcgc aagagccgtt
     2101 tgatgcgcca cttggccacg cttgagaagc aaagcgactg attgagcaat cattaattaa
     2161 attggggata gaaatgcatt cagtgcattt ctatccccaa tttctcttta attctgtatc
     2221 cttttagagc ggcataaacc gctctattct gaatttgatt tgatgtggct aataatccac
     2281 tgaaaaggga ggtaggtcag cacggcgcct gttttatgcg tgatataatc ctggctggca
     2341 tgccccatat tcaggtgtaa cccatgtttg aagcagagca gcaagctatc gaaaagagca
     2401 ttatcgctta ttgtgctcag agtggtctgc ctgagccagg accgctgcaa tggagcccca
     2461 tccccttctc aggggagtgg ggtatatcta cttctttctt ccagttggct gcccaagaag
     2521 cgcgccaagg gaagagcggt ggtgttccgg taccccagcg cgcggcggag atcgccgccg
     2581 gtgtggcggc ctatctaggc acaccgcctg gttattctcg cgttgaagct gtcaaggcat
     2641 acctgaattg ttacttttca gcttccgaat acgctcgcag ggtggtagat actgtgctga
     2701 ctcagggtgc tgactttgga cgaggcgcag taaagggcga gcgcgtcatg gtggagtacg
     2761 ccaacaccaa cacgcaccat tccttccaca tcggccatgc acgcaatgca ttattgggcg
     2821 aggctctagc tcgcctagtg gagtttgcgg gctttgaaac gatccgcgcc tcgtatccag
     2881 gcgatatcgg cctgggcgtg atcaccgtgg tgtgggctta ccagaagttt tacctgggac
     2941 aggaaccctc tggggtgcat gagcgcggcc agtggctgtt aaaaatctac gttgaagcta
     3001 ccaaactgct cgaacccagg gaggacgaaa ccccggaaga aagagcccag cgtgaaagtt
     3061 atgaaactga gcggcgtgag atttaccgcc gttgggatgc caatgacccc cgggtgcgag
     3121 agctatggcg gatgacccgc cagtggagcc tggatgagca gcttgctatc ctggatatgc
     3181 tcgatattca catcgacgtc tggttcttcg agagcgaagt ggatgaatct gccaaaacca
     3241 ttgtggatga actggtggcg ctcggcgtag ccgatgatga gcgctccaac ggtggcccgg
     3301 tgattgtcaa gatcgatgaa aagcttggct tgaagaaaga gaaatatcgc tccaatatca
     3361 ttttgcgcag cgatggcact accctttacc tgacgaaaga cctagcctta gccaaagaga
     3421 aatttgaaaa acaccatgtg gatcgctcga tctacgtgat cgacgtacgc cagagccttc
     3481 acatgcagca gaccttcaag atcctggagc tttggggctt caagcaagcc gaaaagtgct
     3541 tccatcttgg ctatggcttt gtcagcctgc ccgagggcgc catatcggcc cggcgcgggc
     3601 gcatcgtgct gttcaaagac gtggcggatg aagccattcg ccgcgtgttg agcgtgattg
     3661 cggagaagaa ccccgagatg cccgagtccg accgccaggt ggttgcccga caggtgggcc
     3721 tgggtgcttt agcctattcc atgctttcgg tggataccaa caaagatatt gtttttgatt
     3781 gggatgccgc tctgaatttt gacgggcata ccgctccgta tatccagaac gcctatgtgc
     3841 gcgccaacag catcctcaaa aaggctggtg gtctgccaac gcctgccagc ttcgatatgc
     3901 ctttttccag ccatgaagta cagctcatcg acttgatctc gcgctttccc caggtcgtcc
     3961 aatcggcggc tggagagtat cgcccgctgg tgatggctaa ttacgcctat gatctggcca
     4021 gcacctttca tagtttttac cacgtcgttc cggtaatcca ggctgataca gaagccatgc
     4081 gagatgcccg cctgcgtttg gtggcagccg ctcgccagac acttgccaat gccctgcggt
     4141 tgctggttat ccagccaccg gaggtcatgt aggcaaaaag aacaggtgct acctttgatg
     4201 ccctatcgct tggaaagaaa atcaatcatt gaggagataa aatagcatat gtcccctacg
     4261 cgcatcctga tcatgggagc cgctggccgc gacttccaca acttcaatgt tttttatcgc
     4321 gacaatcccg attatgaagt ggtggctttc acggcaaccc agattccaga tatcgaaggg
     4381 cgcctctatc cgcccgaatt gtctggccca cactatccca agggcattcc cattttctct
     4441 gagaacgagc tggtaaagct gatccgcgaa atgcaagtcg acatggtgga ttttgcctac
     4501 agcgatgtgc ctcacgagta tgtcatgcac aaggcctcgc ttgctctttc cgctggggcg
     4561 gacttccacc tgttggggat gggcagcacg caactcaaat ccagcaagcc ggtaatctcg
     4621 gtatgcgcgg tgcgtaccgg ctcgggcaag agccagacca cccgccgagt atccttgatc
     4681 ctgcggggca tgggctataa ggtggctgtc atccgccatc ctatgcccta tggtgacctc
     4741 accaaacagg tggtgcaacg atttgccgat tacggtgact tggacaaata caattgcacc
     4801 atcgaagagc gcgaggaata cgagccgcac ctggataatg gcatgattgt tttcgctggc
     4861 gtggattacg aacgtatcct gcgccaggcg gaacaagagg tggatattat cttgtgggac
     4921 ggtggcaata acgattttcc cttctatgtc tccgacctaa aaatcgtggt ggctgaccct
     4981 caccgccctg gtcacgaatt aagttaccat ccaggggaga ccaatgcccg ctctgcggac
     5041 gtgttcgtca ttaataaggt ggataccgct cccccagaga acgtcatcaa agtgcgcgag
     5101 catctgcgct cgctcaatcc gaacgccgtg atgatcgagg gggcgtcccc gctgttcgtg
     5161 gatgaccctg gtgcgatcca gggcaagcgc gtgctggcgg tcgaggacgg gcccactctg
     5221 acgcatggtg agatggcata cggcgcagcc tgggtggctg cgcgccgttt tggtgccgct
     5281 gagatcgtgg accccaggcc gtttgcggta ggatccatta aggccacctt tgaaaaatat
     5341 cccaccatag gggctgtcct gccagccatg ggctatggcg gacagcagat caaggagcta
     5401 gaagagacca tcaaccgctc caatgccgat ctggtgctga tcggcacccc gattgatctc
     5461 aatcgcctgg ttaaaataaa taagccaaac cagcgtgtgc gctacgagct gcaggagatt
     5521 ggtcagccca cgctgcaaga tcttttaaag gctaagtttg gtaaataggc tggtaatcca
     5581 agatcctaaa gatcaccgag atctttaggg tctttatttg gtgtattatg gaaaacacac
     5641 ttgctctcac aatcacccat cgttattggc cgcgggcgaa tggattgctg cgcgacatac
     5701 tacttatcgg tgcaggcagc ttccttgttg ctgctctggc tcaggtgcgc atcccgctgc
     5761 cgttcacgcc ggtgccgctc actggtcaga ccttcgccgt tctcctggtt ggcgctgcgc
     5821 tgggcagccg gcgtggcgcg gccagccttg cgctttatct gagcgcaggc ttgttaggct
     5881 tacccgtttt cactggttta gggagcggtc tggctcattt cgcaggccct acgggcgggt
     5941 att
//
LOCUS       DBNGSX010000009         3538 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1039262, whole genome shotgun sequence.
ACCESSION   DBNGSX010000009 DBNGSX010000000
VERSION     DBNGSX010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1039262"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(430..2367)
                     /locus_tag="AC16CB_00295"
     CDS             complement(430..2367)
                     /locus_tag="AC16CB_00295"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3340931.1"
                     /translation="MILRFYLLGPAIVTWKHTPLNITRRQVRKLLYLLATQESAVPLE
                     RLHYLFWSDKPESFCRSNLSHLLTHARRSLPNKDILTVTNSIAMLDPSQTWSDVGEFK
                     NLVKNEARIENLPSLVKAYDLYRGPYLDGVQLPGGRELEYVVEQERYTLERQYLNGLI
                     KLIEIETQRGKLENALEYAYKYLEIDNLSEEVHRQLIRLHAWMGNQDRAIRQYKLCEE
                     ILAREIQATPSKKTQAMYQEALSGNLSEYRVEKKPPEYKTSKVREIPISQRLSHLDQL
                     QKNFDNFFLGVGGVVLLSGEIGVGKNNLIQESLLRNQNNSLILHAHCDPGSESISFLP
                     LIDIATDVSNYQDKIRFLNPDSTHEIAQIAESISLTTSKWTEASEITKIDQKEYFFKL
                     ISRFFEKLLSASNGVILCLEDLEWADENTLDLILHLSSRFSHRKFLFIGSLCCKRDKK
                     IQDFEHHMQLTRSNVTLINVSGYGIHEIHNLVKYWIGSFNGSQAFSEKLLQICGGNPY
                     YLHEIVKWIAESGLSAKELDENPNIPIPNTISKTIEARLGRLEPVEREVLEYASLLGR
                     KFRFEELHSCSDLPLTHILDTLDNLVNRHLLVVHSTNYQFRHELIRFFILNSMNPARR
                     QAMEQNFVHEPDANLVATILE"
     gene            complement(2580..>3538)
                     /locus_tag="AC16CB_00300"
     CDS             complement(2580..>3538)
                     /locus_tag="AC16CB_00300"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR3340932.1"
                     /translation="PILSFGIQLIQALQTLSPALDVPMRFFTFLGTIEFYILIIPVFY
                     WLVDSQLGFRILLALLSTDILGTAFKQLLHQPRPYWVGDVKAMGDEISYGIPSTHASD
                     SLTVWGFFAYQMKKNWLWVASAGLVFLISLSRLYLGVHFPQDVLGGWLIGAFVLYLFI
                     KLEPSVLSWLKNLSQLTQIGIAFGISVLVILVGLLVGLAIASTPDPAEWAQYATQARS
                     PSHYYTLGGVFFGAASGFVLMWHRARFGTQGSGAQKAGRYLLGIAGVLVIYMGLDAVF
                     GLIAADETAAGYLLRYIRYASLAFWMTFGAPWAFLKLKLAHK"
BASE COUNT          848 a          876 c          911 g          903 t
ORIGIN      
        1 ccgccaccgc cccccccgcc cccgccggga cctggccctt tggccagccc caggctggag
       61 gtcagcatca aggctagaat caagatcata gaaattgcta ttcgcttaga catggtattt
      121 cactcccttc tcatttttct ttccactggg catttaggtg gttgaatcgt tgtgtccaca
      181 gccaaccgga tcgaggtata ggctacctcg ggaacgtgag ccgtgatttt ctccccggca
      241 atgcccttac cgcttggttg gctgttggtc attaccacta tagcaagaac aggtcaacgc
      301 tcttgctaca ttaatgctac aagagtggcc gttgtggaat tcttgtattc gaaggcgagt
      361 atttatcttt gtcgtgccaa tgcaattgag cggttcgctt tgcttgattc cccctggatc
      421 agcgagacct cattccaaaa ttgtcgcaac cagattcgca tccggttcgt gcacaaaatt
      481 ttgctccata gcctggcggc gcgctgggtt catgctattc aaaatgaaga agcggatcaa
      541 ttcgtgacgg aactggtagt ttgtagagtg gacaaccagc aggtgccggt taactaaatt
      601 gtccagggta tcgaggatgt gtgtcagggg taggtcgcta caagaatgta attcttcgaa
      661 tcgaaacttg cgacccagca gggaggcata ttccagaact tcccgttcaa ccggctccag
      721 gcgaccaagc cgcgcttcga tcgttttgga aatggtgttg gggatcggga tgttgggatt
      781 ttcatctaat tcctttgcag acaacccgga ttcagcgatc cattttacga tctcgtgcag
      841 gtagtaagga ttgcctccac aaatttgcag gagcttctct gagaaggctt ggcttccgtt
      901 aaagctccct atccaatatt tcaccaggtt atggatctca tggatgccat atccagatac
      961 attgatcaag gttacgttgg agcgggttaa ctgcatatgg tgctcaaaat cctggatttt
     1021 tttatcacgc ttgcagcaaa gcgaacctat aaacaggaac ttccggtggc tgaacctgct
     1081 ggatagatgc aggattaaat ccaacgtatt ctcatctgcc cactctaaat cttcgaggca
     1141 gagaatgacg ccattagaag cgctcaatag cttctcgaaa aaccttgaaa tcagcttgaa
     1201 aaaatactcc ttctggtcga ttttagtgat ctcactggcc tcagtccatt tagatgtggt
     1261 aagtgaaatg gattctgcta tctgggctat ctcatgtgtt gaatcggggt ttaagaaccg
     1321 gattttatcc tggtaattgg aaacatctgt tgcaatatcg attaagggga gaaatgagat
     1381 tgactcagac cccgggtcac agtgcgcatg tagaatgagc gagttgttct ggttgcggag
     1441 caggctttcc tggatcaggt tgtttttacc cacccctatc tcgccgctaa gcaacaccac
     1501 cccaccaacg cctaagaaaa aattgtcaaa gtttttctgt agttgatcta aatgagataa
     1561 cctttggcta atcggaattt ccctcacttt gctggtctta tactcgggtg gttttttttc
     1621 aacgcgatat tcgctcaggt ttccgcttag tgcctcctgg tacattgcct gcgttttctt
     1681 ggatggtgtt gcctgtatct ctcttgcgag tatctcttcg cataacttgt actgcctgat
     1741 ggcgcgatcc tggttgccca tccaagcatg taacctgatc aattggcggt gaacctcttc
     1801 agacaggtta tcaatttcaa gatatttgta agcatactcg agggcgttct ccagtttccc
     1861 cctttgtgtt tctatttcga taagtttgat caagccattt aagtactgtc tctccagcgt
     1921 gtatcgttcc tgctcaacca cgtactccag ctcccggccg cctggcaatt gaactccatc
     1981 cagatatgga cccctataca agtcataggc tttgaccagt gacggcaggt tttcaattct
     2041 cgcctcgttt tttaccaggt ttttaaattc ccccacatcc gaccatgtct ggcttggatc
     2101 aagcatggct atggaatttg taacggttaa gatatctttg tttggcaggg aacgccgggc
     2161 gtgcgtgagt agatgcgaga gattgctccg gcaaaaggat tcgggtttgt cactccaaaa
     2221 taaatagtgc agccgttcga ggggaacagc agactcctgt gttgcgagca gatataagag
     2281 ttttctaact tgccgacgtg tgatgttaag aggggtgtgc ttccaggtga cgatggcagg
     2341 accaagcaaa tagaacctta atatcaccgg ccttcatcct tgtttcttgg tacctaatct
     2401 gatactcgcc tagagtaaaa ctaactatat atgtgtatat ataatacagg taattgcgtt
     2461 ctgtgtcaat aattttgtgt atcagctgtg tatataaaca ctaaaagtta caccgcataa
     2521 aggtgtttat ggttacgcag gggggggtgt acaggtaagt aagtctgaat tcgggaaaat
     2581 tatttatgag ccagcttcag cttcagaaag gcccagggcg cgccgaaggt catccaaaag
     2641 gcgaggctgg catagcggat atagcgcaag agatagccgg cggcggtttc atcggcggcg
     2701 atcaggccga agactgcatc caggcccatg tagatcacca gcaccccggc gatccccagc
     2761 aggtagcgcc ccgctttttg ggcgcctgac ccctgggtac caaagcgggc tctgtgccac
     2821 atcagcacga agccggaggc tgcgccgaag aacaccccgc ccagggtgta gtaatgggag
     2881 ggcgagcgcg cttgcgtggc atattgcgcc cactccgccg ggtcgggtgt ggaggcgatt
     2941 gccaggccaa ccagcaggcc gaccaggatc accaataccg agatgccgaa ggcgatgcca
     3001 atctgggtca gctggctgag gtttttcagc cacgacaata cgctgggctc gagtttgatg
     3061 aacaaataga gcacgaaggc cccgatcagc cacccgccca gcacgtcctg ggggaaatgg
     3121 acgcccaggt acaggcgcga caggctgatc aggaaaacca gccccgcgga ggctacccag
     3181 agccagttct ttttcatctg gtaggcgaaa aatccccata cggtcagcga atcgctggca
     3241 tgagtggagg ggatgccgta ggatatctcg tcgcccatgg cttttacatc acccacccag
     3301 taggggcgcg gctggtgcag cagctgctta aaagccgtgc ccaggatgtc ggtgctgagc
     3361 agcgccagca agatccggaa tcccagctgg ctgtcgacca gccagtaaaa aaccggtatg
     3421 atcaagatgt aaaactcaat cgtccccaag aacgtgaaaa accgcatggg gacgtccaga
     3481 gccgggctca gggtctgcaa agcctggatg agctggatgc cgaagctcag gatagggt
//
LOCUS       DBNGSX010000010        11358 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_276, whole genome shotgun sequence.
ACCESSION   DBNGSX010000010 DBNGSX010000000
VERSION     DBNGSX010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11358)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11358)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11358
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_276"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            963..2363
                     /gene="ppcB"
                     /locus_tag="AC16CB_00305"
     CDS             963..2363
                     /gene="ppcB"
                     /locus_tag="AC16CB_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylphosphate carboxylase subunit beta"
                     /protein_id="HIR3340933.1"
                     /translation="MKDLRDYIETCEAEGELKRVKAEVDWNLELSHIAKLNEERSGPA
                     LLFENVKGYSSPVLTSACTTTARLALIMGMPKTSSLVEMMREWVKRSADKVPSKWVDS
                     APCKENIDKGDSVDLEKFPVPWVYPKDGGRYFGTAVYVVSKSPKTGWINLGTYRLQML
                     DKKTLGTQFIKGKHADIMLKEYGELKKPMPVAAIIGGDPLMFLMGAARLPAYESEYDF
                     AGAIRGTPIDVIKGETVDLPVPATAEIVVEGEVDPVAMLPEGPFGEYTGYYSGIGTTP
                     RPFIKVNCVTYRNNPILWSTTVGRAVTDTHMTMALTYGATLWQELVRMRIPGVKAVYC
                     PPEGSGRFIAIISVKQMYPGHAAQVGTAAISTEMGAYGLKTVIVVDDDIDPWDLPRVL
                     WAISFRFQPSRAEFIKRGRSTPLDPSLPLDAREITSRLIIDATIPYEWKEKPIPIDMD
                     PDMMKRVESRWSELGL"
     gene            2464..3741
                     /locus_tag="AC16CB_00310"
     CDS             2464..3741
                     /locus_tag="AC16CB_00310"
                     /inference="COORDINATES: protein motif:HMM:NF049415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HIR3340934.1"
                     /translation="MEIAVVGMLDEREPALRLLKERIERRGHKVTLIDVSIGTGGIMP
                     DLKPDITCEEVAQASGSSIEEIRKMLAKERDTATSLMAEGLAKKTLELYQAGRLNGML
                     GVAGMTGTFMTLTAMKSLPFGVPKVLISSVAAMPAYASRFAEYFSIRDITIIHSVVDT
                     VGWNPLVRSLMINGAGAICGMVEDVEPRLKEGKPSVAMTELGFCDKGAHYVRDLIQDS
                     YNIVSFHATGLGDRAAEDLVGQGLFDAFVDLVPAGVSERLLGGNRAAGPDRLEGACRQ
                     GTPYILAPCGFDMISCGPIERRDKGDPVWVSRKLAERKLLIQDAMRVQARTSVDEMAE
                     IAEAVAEKLNRHKDKRLVKFLIPTKGFSSLSVEGGQLYDPEVDRAFAQELRKRLAPEI
                     QVIEVDAHINEMAFAQAVAEALETSVRLRDSVK"
     gene            3949..5796
                     /locus_tag="AC16CB_00315"
     CDS             3949..5796
                     /locus_tag="AC16CB_00315"
                     /inference="COORDINATES: protein motif:HMM:NF006153.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HIR3340935.1"
                     /translation="MSNPNRFPFAREIEIPSEMQGWEEMYPAHRMFSPERREWEETQF
                     WYADKIHAPDPMVPLDEVFQQAWQIALSQHTTRIFCIPPAQGIAQRMVGCYMYICAIT
                     PPPPEVIQEKAELFPKRTFPLFESYGTLWEDWLTRFQTLGKELRSIKVPTEFPKYMPD
                     FNSATTLPAVTPAQELLEAFNTAVNIIYRGWQYHFNYLNVTYLSYLMFADVARQLFPG
                     ISESTIGKMVAGAEVSMFRPEEELCRLSRLAHAESKVADILRSDRPAASKIQSLRESE
                     AGRHWLDELERIKDPWMYVSCGAGWYHYEGSWITKLDVPFSYLKGYIERLDKGEKIER
                     SFVAIAEARDRVVEAYRKLIKTPEDQQSFEGAYKNTRTIYRYAEDHLFWVEHWFHTLW
                     FEKMREFARLFVKQGVLRDEEDFFMFNRLEVPYLIEDFSTSWALGEGIPTLNWADKAQ
                     KRKKILDAAAKWNPPPALGIPPDVVAEPFTVMLWGITTERVQDWLKGTSAKPGDVNEL
                     KGFASSAGVAEGKARVLKLLEEIVNIKQGEILVVGNTNPAWAPIFSKIKGAVTDIGGI
                     TSHAAIVCREYGIPSVTGTGTATSTIKTGDIIRVDGDSGMVTIVKRASS"
     gene            5844..6911
                     /locus_tag="AC16CB_00320"
     CDS             5844..6911
                     /locus_tag="AC16CB_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019986769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR3340936.1"
                     /translation="MDYVLDFSQIDKNLLAMVGGKNASLGEMLKAGIRVPPGFAVTTE
                     GYQEFLTGAGLDDVICGILDAVDPEDMASLDNASAQAQALINGAPMPSAIADAIRESY
                     AGLCEGCQSQDLPVAVRSSATAEDLPDASFAGQQDTYLWVTGGDQVIKMVQKCWASLF
                     TPRAISYRIKIDCSHITTLMSVGVQKMVNSRTSGVMFTLNPTNGDPSKVMVEASWGFG
                     EAVVSGIVTPDSFLVDKIVSEINQRTISAKTVECVRDGVVGVIHRDVPPERQTTPSLS
                     DEEIKELVNLAKQIENHYGRPQDIEWAIDIDFSPPENIFILQSRPETVWSQRPNKPLL
                     GGKTGFELLMDKALTTIKVRK"
     gene            7058..7711
                     /locus_tag="AC16CB_00325"
     CDS             7058..7711
                     /locus_tag="AC16CB_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR3340937.1"
                     /translation="MFRHGHVANHQGDVSLLPEGRQVAFNAGASLADQVPDNVAMTIL
                     FSPSARTRETAEELARGLAGGLASVNKLGARVLQPQPEDAIRNFQFIVDGREMMPTDG
                     MHASLPPSAEHDPFFQAFWQDQDDPIDYWLTHPSQSAEQPVAVAARMRTFFGSLLQFP
                     FPGIFLLVTHSGPMRAFLREALGADPGEPEFCEAFQVNANETLFRGQRAHFSISQPP"
     gene            7731..8249
                     /locus_tag="AC16CB_00330"
     CDS             7731..8249
                     /locus_tag="AC16CB_00330"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3340938.1"
                     /translation="MHVNFCARCGAELTTRFESGRDRPACPECGYIVYSNPTAVGIVV
                     ATRGDKLLLVHRRHPPLAGYWAPPAGHIEFDESIQEGTAREAKEETGFDVSIGRLLNV
                     YSQANTGVVLVVYDGQIIGGDMAADEEEVMEVRFFGRDELPRQPAPTNGTIVERMFFQ
                     SIEELFEDFRNR"
     gene            complement(8236..8751)
                     /locus_tag="AC16CB_00335"
     CDS             complement(8236..8751)
                     /locus_tag="AC16CB_00335"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR3340939.1"
                     /translation="MTADFETDPQSSDLHRRLQSFLADQTTMTLSSVTDEGNPHSCDL
                     FYAHTSKYTFYFLSDPKTLHSQNIARMPRVSVTIHGLSRGWEDIRGVQMEGVAMRVSE
                     PVERLRAFALYAGKYGFIKRWLTSVDTLGVMIEGLGVIELYRFSPQWLRFIDNSLGFG
                     HKDEINISTGS"
     gene            complement(8748..9344)
                     /locus_tag="AC16CB_00340"
     CDS             complement(8748..9344)
                     /locus_tag="AC16CB_00340"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HIR3340940.1"
                     /translation="MAERFVVGISGASGAIYGIRLLQALRTIPDVESHLVLSSAAQKT
                     IAQETDWDVKDVLALASKVYGNNEIGAAIASGSFAARGMVIIPCSIKTLSAVSNSYGT
                     DLISRAADVTLKEGRTLILVVRETPFHLGHLRLMTNAAEMGAVIFPPLPALYARPRTL
                     DDMINFTVGRILARLGVDNDLFSQWTGLDQKPTNSDNA"
     gene            complement(9347..10642)
                     /locus_tag="AC16CB_00345"
     CDS             complement(9347..10642)
                     /locus_tag="AC16CB_00345"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HIR3340941.1"
                     /translation="MEVTYFNREIETASREEMVGHQTGRMQVLLGEMLASNAFYGRKL
                     REAGFTQARDFKSLADITQLPFTRKQELVDDQETRPPFGTNLTYPLERYTHIHQTSGT
                     TGKPMRWLDTPDSWDWWARCWSAVYRAAGISESDRIFFAFSFGPFIGFWAAWAGSDKI
                     GALAISGGAQDSVQRLRNLIDLGATALCCTPSYALHLVEIAQKEKVDLRASGVRRIIV
                     AGEPGGSIPGTKARIEEAWGAKLFDHTGATEVGAHGFSCLAESGVHLNEGEFLIEAID
                     PQTGMPADEGELIITNLGRLGSPIIRYRTGDQVRLNRATCECGRTFVRMDGGIIGRID
                     QMLIVRGVNIFPSAIEAIMRSYPEVIEFAVEVYSIEAMDELAIKAEIAGENPQAIANS
                     IARDASHRLGLRVPISLAPPESLPRFELKARRLVDRRSK"
BASE COUNT         2769 a         2895 c         3008 g         2686 t
ORIGIN      
        1 gcatcgaaat catcaacgct gagatagtcg aattcaccct ttagttgaat aatggttact
       61 ggaactcgcc cctgtgcctt agaaattatt atctccattg atatttctcc cgattgtctt
      121 gtgcaatact caataaatag ggcaatttac tctaattagt aaggcccaat cgcccaaaat
      181 atggctgaaa gcaaatcgct cacacccgct tgccccattt aattcatacg gttcataatt
      241 tcggggacat tatactatac tggataatat aaggcgtctt taaattgcgg gggttcgatt
      301 tccttgattc aggccaagca ggccaactaa atgggatggg cgcttttgat gatggctaat
      361 atatcaccaa cacacacttt tcacccaagc tgacaaaaac atggatttag atggcactcc
      421 atctaaaaca aggactgaaa atgcaatatt atgaccgggg cgttggtcag gaatgtgcac
      481 actggacggg ataacttgac ttcatctaag ttagtttgag aaatatttca caatctattt
      541 tgtgcaagat gttactttta tcaattttta taggatattt atcactagga tcagacgcat
      601 catattgtta taaacacttt gtataggctg ggactggctt gctgtcccag ctatagtctg
      661 ttctctttat taaatatcaa atcaatgtat tgcttacgtt atggttaata atttaggaga
      721 attcactccg cgtggtagtt cctttacaag tgtcgtctaa gcagcaagca gcccatttga
      781 tcgaatagca tttgaccaat caaacacgtt tccgcaagtc tcgcttgcgg agactctcag
      841 ataagctcgc cgagacttaa catcgccata gcagcaacgg agatcactgg ctggtgattc
      901 ttcccgttaa tagcacaaaa cgttatgcaa ctcaaatact aacggtagaa aaggagaaaa
      961 aaatgaagga tttgcgagac tatatcgaga cgtgcgaagc ggaaggcgaa ctcaagcggg
     1021 tcaaagcaga ggtggattgg aatctggagt tgagccatat cgcaaaactc aacgaagagc
     1081 gcagtgggcc ggctcttttg tttgagaacg taaagggcta ttccagcccg gtgttgacca
     1141 gcgcctgcac gaccacagcc aggctggcct tgattatggg catgcccaag acttccagtt
     1201 tggttgagat gatgcgggag tgggttaagc gatcagcgga caaggttcct tccaaatggg
     1261 ttgattctgc cccttgtaag gagaacatcg acaaaggcga cagtgttgac ctggaaaagt
     1321 ttccggttcc gtgggtctat cctaaagatg gtgggcgata ctttggtacg gcagtgtatg
     1381 tcgtgtccaa atctcccaag accggctgga ttaatctcgg cacgtaccgc ctccagatgt
     1441 tggacaagaa aacgctgggt acccagttta tcaaaggcaa gcatgcagac ataatgttga
     1501 aggaatatgg ggaactcaaa aagcccatgc ctgttgcggc aatcattggc ggcgatccat
     1561 tgatgttctt gatgggggca gcccgtttgc ctgcttatga atctgagtac gattttgccg
     1621 gcgccattcg cggaacgccg atcgatgtga tcaaaggcga aacggttgat ctgcctgttc
     1681 cagctacagc cgagatcgtg gtggaaggcg aagtggatcc tgtggctatg ctccctgagg
     1741 gaccgtttgg cgaatatact gggtactatt ccggtattgg taccacgccc agaccattta
     1801 tcaaggtcaa ctgtgtcacc taccgcaata acccgatttt atggtctacg acagtaggcc
     1861 gggcagttac cgacacccac atgaccatgg ccctcaccta cggtgctacc ctgtggcagg
     1921 aattggtgag aatgcgcatt cctggcgtaa aagcagttta ttgcccaccg gagggctcag
     1981 gccggttcat cgccatcata tcggtgaaac agatgtatcc cggccatgct gcccaagtgg
     2041 gcaccgccgc catctcgacc gaaatgggtg cctatggact caaaaccgtc attgtcgtgg
     2101 atgatgacat tgacccgtgg gatctgccgc gcgttttgtg ggcgataagc ttccgcttcc
     2161 agccctcgcg tgccgagttc atcaagcgag gccgttccac ccccctcgat ccatccttgc
     2221 cgcttgatgc ccgggaaatt acatcgcgac tcatcataga cgccacaatc ccatatgagt
     2281 ggaaggaaaa acccatcccc atcgatatgg atcccgacat gatgaagcgc gtcgagtcca
     2341 gatggagtga gttaggtctc taaatcccag gttccttcag tttccgcggc tccttcgttt
     2401 aatagaaata tcaacgaacg aacccggaat actgaaggaa ctattaataa tacgaggatc
     2461 aacgtggaaa ttgctgttgt gggcatgttg gatgaaaggg aaccagcctt gcgcctgctc
     2521 aaggaacgta ttgagcggcg cggacacaag gttacgctca ttgatgtaag cattggcact
     2581 ggcgggatta tgcccgatct caagccggat ataacgtgtg aagaggtggc acaagccagt
     2641 ggaagttcca tcgaagaaat cagaaaaatg ctggctaaag aaagagacac cgccacatcc
     2701 cttatggcgg agggtctggc aaagaaaacc ttggagttgt accaagccgg acggctgaac
     2761 ggtatgctcg gtgtagcagg catgactggc acattcatga ccttgacggc catgaaatct
     2821 ttgccgtttg gcgttcccaa ggttctcatt tcaagcgtag ctgccatgcc cgcttatgcc
     2881 agccgttttg ccgaatactt cagcattcgc gacattacga tcattcatag tgttgtggac
     2941 accgttggct ggaacccatt ggtaagaagt ttgatgatca atggcgccgg ggcgatctgc
     3001 ggcatggtag aagatgtgga gccgcgattg aaagagggca agccctcggt cgccatgacc
     3061 gagcttggct tttgcgataa aggggcgcat tacgtacggg acctgataca agatagctat
     3121 aacatcgttt cgtttcatgc cacaggattg ggagatcgcg ccgccgaaga tttagtcgga
     3181 caggggttgt ttgacgcttt tgttgacttg gtgccagccg gcgtgagcga aagacttttg
     3241 ggaggcaacc gcgcagccgg ccctgaccgg ctggagggcg cttgtcgtca ggggacacca
     3301 tatatactgg ccccctgcgg attcgacatg attagttgtg ggcccatcga gcggcgggat
     3361 aaaggtgatc ccgtttgggt ttcgcgcaaa ctggcggaaa ggaaactttt gatccaggat
     3421 gcgatgcggg tccaggcgcg cacctcggtg gatgaaatgg ctgaaatagc tgaggcggtt
     3481 gcagagaagt tgaaccggca caaagacaaa cgcttggtca agtttttgat acccaccaaa
     3541 ggattttcct ctttgagcgt cgaaggcgga caattatacg atccggaggt tgaccgggca
     3601 ttcgctcagg agctgcgaaa gcggttggct cccgagatcc aagtgatcga ggtggatgca
     3661 catatcaacg aaatggcatt cgcccaagct gttgccgagg ccctggagac gagcgtgcgg
     3721 ttacgggatt cggtcaaatg aggtgataac gaggaggtga agataacgga tatcctatga
     3781 attgagagca ggttatatgg ggaaccggcg cgtgttgcgc ctttctacgc ggttttctgc
     3841 tggagcggtg atagttgtat cagcaatcaa cctttgctca attgcctgac catgtgaata
     3901 acaaacatta ggcaatcaaa acaatcaaca aagccaagga ggtaaagcat gtctaaccca
     3961 aacagattcc ctttcgcgcg ggaaattgaa atcccctctg agatgcaagg ctgggaggag
     4021 atgtatcctg ctcaccgcat gttcagtcca gaaagacgag aatgggaaga aacgcaattc
     4081 tggtatgccg acaagatcca cgcgccagac ccgatggttc ccctcgatga ggtttttcaa
     4141 caagcctggc aaatcgccct ttctcaacat accacccgca tattctgcat cccgcctgcc
     4201 caaggcatcg cccaacggat ggtcggctgc tatatgtaca tctgcgccat cacgccgccg
     4261 cctccagagg tgatccagga aaaggccgag ctgtttccca aacgaacttt tccgctattt
     4321 gaaagctacg gcaccctgtg ggaagattgg cttactcgct ttcagactct gggtaaggaa
     4381 ctgcgctcca tcaaagtccc cacagaattc ccgaaataca tgcccgattt taactcggcg
     4441 accactctac ccgccgtcac gccggcgcaa gaattgctgg aggcatttaa cactgccgtc
     4501 aacattatct accgcggctg gcagtatcat ttcaactact tgaacgtgac ctacctgtcg
     4561 tacctgatgt ttgcagatgt ggcgcgtcaa ctcttcccag gcatcagcga aagcaccatc
     4621 ggtaagatgg tagccggagc ggaagtttcc atgttccgtc cggaagagga actgtgtcgc
     4681 ctgtcgcgtc tggctcatgc cgaatcgaaa gtggctgaca ttcttcggtc tgaccgcccc
     4741 gcagcgtcca agattcaaag ccttagggaa agcgaagcag gccggcattg gctggatgaa
     4801 ctcgaaagaa tcaaagatcc ctggatgtac gtctcgtgcg gtgctggctg gtatcactat
     4861 gagggcagtt ggatcaccaa gttggatgtg ccgtttagct acctcaaagg ctacattgag
     4921 cggttggata agggcgagaa gattgaaaga tcgtttgtcg ccattgccga agcacgcgac
     4981 cgtgttgtgg aagcctatcg taaactcatc aagacgcctg aagaccagca gtcgttcgag
     5041 ggcgcatata agaacacgcg cactatctat cgatatgctg aagatcacct cttctgggtt
     5101 gagcactggt tccataccct gtggttcgaa aagatgcgcg aattcgccag acttttcgtc
     5161 aagcagggag tgctgcgcga cgaggaagac tttttcatgt tcaatcgcct cgaagtacct
     5221 tacctgatcg aggatttttc cacttcctgg gcgctgggcg aaggtattcc cactcttaat
     5281 tgggcggaca aagcccaaaa gcgaaagaaa attctagatg cagccgccaa atggaacccc
     5341 ccgccggcat tgggcatacc gccagatgta gtcgctgagc cgtttacagt catgctttgg
     5401 ggtattacga ccgagcgggt acaagactgg ttaaagggca cgagtgccaa gccgggcgat
     5461 gtgaatgaat taaagggctt tgcttcctcg gctggcgtgg ccgaaggcaa ggcgcgcgtc
     5521 ttaaaactgc ttgaagagat tgtgaacatc aaacagggag agattctggt cgtcggcaat
     5581 accaatccag cttgggctcc catattctcc aagattaagg gcgcggttac cgacattggc
     5641 ggaatcacgt cacatgccgc catcgtctgc cgcgaatacg gcataccgtc cgtaaccggt
     5701 accggcacag caacatcaac gatcaagacg ggcgacatta tccgggttga tggcgatagt
     5761 ggtatggtga ctattgtgaa acgggcgagt tcttgaagca cgctttttct caatggcata
     5821 taaacctacc atggaggtag cgtatggatt atgtgttgga ttttagccaa atagacaaga
     5881 atctgcttgc catggtaggc ggcaaaaatg ccagtctggg cgagatgcta aaagctggca
     5941 tcagggtgcc gccgggcttt gccgttacta ctgagggcta ccaggagttt ctcacgggtg
     6001 caggactgga tgatgtgatt tgcggcatct tggatgccgt agatcctgaa gacatggctt
     6061 cgcttgacaa tgccagcgcc caggcacaag ccttgattaa cggcgctccc atgccctctg
     6121 caattgctga cgcgatacga gaaagctatg ccggactgtg cgaggggtgt caatcacaag
     6181 acctccccgt tgcagtgcgt tcaagcgcca ccgccgagga tttgcccgac gcgagttttg
     6241 ccgggcaaca agacacctat ctgtgggtta caggcggtga tcaggtaatc aagatggttc
     6301 aaaagtgctg ggctagtcta tttactccaa gggctatcag ctacagaatc aagatagatt
     6361 gctctcacat taccacactg atgagcgttg gcgtgcaaaa gatggtgaac tcgaggacat
     6421 caggcgtaat gtttacgctc aatccaacga atggcgatcc ttcgaaggtg atggtcgagg
     6481 ccagttgggg ctttggagag gcggttgtat ccggcatagt aacgccggat tcatttcttg
     6541 tggacaagat cgtctctgag atcaaccaga ggacgatctc agccaagacc gttgaatgtg
     6601 tgcgagacgg cgtggtgggc gtgatacatc gtgacgtgcc gcctgagcgc cagaccactc
     6661 ccagcctgag cgacgaagag atcaaggaat tggtcaattt agccaagcaa atcgaaaacc
     6721 attatggcag gcctcaagat attgaatggg cgatcgacat agatttctct cccccggaaa
     6781 atatcttcat cttgcaaagc cgccctgaga cagtgtggag tcagcgtccc aacaaacctc
     6841 tcctgggcgg aaaaacgggt ttcgagttgt tgatggataa agccctaaca accattaaag
     6901 tgcgcaagta gctcgttaca gtaggccctc gaggttctcc ttgaggcctt gagggtctgc
     6961 ggtattaatc ttggaagtta cgcagttgaa tacgtccttt cctcttcaac tgcgtaagtc
     7021 ctaaatctgg agactgccta tcgcttcaag gatctttgtg tttcggcatg ggcacgtggc
     7081 aaaccatcag ggcgatgttt ctctgttgcc agaaggccgc caagtggcct ttaatgccgg
     7141 ggcttccctg gctgatcagg tccccgataa cgtcgctatg acgattcttt tcagtccctc
     7201 cgcccgcacg cgtgagaccg cagaagagct cgcccgtggt ttggctgggg gtttagcaag
     7261 cgtaaacaag ctaggggcgc gcgtgctgca gccgcagcca gaagatgcga ttcgcaattt
     7321 tcagtttatc gttgatggca gagagatgat gccgacggat ggaatgcacg catcgctccc
     7381 accttcagcc gaacacgatc cgttttttca ggccttctgg caggaccaag acgacccgat
     7441 tgactactgg ctgactcatc cgagccaaag cgcggaacag ccagtcgccg ttgctgcgcg
     7501 aatgcgcacc ttttttggct cacttctcca attccccttc ccggggattt ttctgctggt
     7561 tactcattcc ggtcccatgc gcgcttttct tcgtgaggcg cttggtgccg atcctggcga
     7621 gcccgagttt tgcgaggcat ttcaagtaaa tgccaacgag acgctctttc gcgggcagcg
     7681 cgcccatttc tcaatctccc agccaccatg aagaattgcg agattttgct atgcacgtaa
     7741 atttttgcgc ccggtgcggc gcagagttga cgacaagatt tgaatcagga cgtgatcgcc
     7801 cggcttgccc tgagtgcggt tatatcgtct atagcaaccc cactgcggtc gggattgtcg
     7861 ttgcgacgcg cggcgacaaa ttactgttgg ttcaccggcg ccacccgccg ctggctggtt
     7921 actgggcgcc gcccgcaggt cacatagaat ttgacgaatc catccaggaa ggaaccgctc
     7981 gggaggcaaa agaggaaact ggattcgatg tatccatcgg caggttgctc aacgtctaca
     8041 gccaggcaaa caccggcgtg gtcctggttg tgtacgatgg ccaaatcatc ggtggcgata
     8101 tggctgccga tgaagaagag gtgatggaag tgcgcttctt cgggcgtgac gagttgccgc
     8161 gccaacccgc gcccacaaac ggcacaatcg tcgagcgcat gttttttcaa tccattgaag
     8221 agctgtttga agatttcagg aaccggtaga gatatttatt tcgtctttgt gcccaaatcc
     8281 gagcgaatta tcgatgaacc gaagccattg cggggaaaac cggtaaagtt cgataacccc
     8341 cagaccctcg atcatgaccc caagtgtatc cacgctggtc aaccatcgct tgatgaaccc
     8401 atacttgcct gcgtacaatg caaatgcgcg caagcgctcc actggttccg agacgcgcat
     8461 tgcaacgcct tccatttgaa ccccgcgaat atcctcccag ccgcgtgaaa gcccatgaat
     8521 cgttaccgag acgcgcggca tgcgggcgat gttttgcgag tgtagggtct tagggtcaga
     8581 tagaaaataa aacgtgtact tgcttgtatg cgcgtagaac aggtcgcatg agtgtggatt
     8641 gccctcatca gtcacactgc tcagtgtcat tgttgtttgg tcggcaagaa aggattgcag
     8701 gcgacggtgc aagtcgcttg attgtggatc ggtctcgaaa tcagccgtca tgcgttgtca
     8761 gagttggtcg gtttttgatc caaccccgtc cattgggaaa acagatcgtt gtcgactccc
     8821 agccgtgcca gaatgcgccc gacggtgaaa ttaatcatat catccagcgt gcgcggacgc
     8881 gcgtaaaggg cgggcaacgg cgggaaaatc accgcgccca tctcagcggc attcgtcatc
     8941 aggcgtaaat gccccagatg gaaaggcgtc tcgcgcacaa cgagaataag cgtccgtcct
     9001 tctttgagcg tcacgtcagc cgcgcgcgaa atcaagtctg tgccgtatga attcgacacc
     9061 gccgagagcg ttttaatgct gcatggaatg atcaccattc cacgggcggc gaaacttcca
     9121 ctggcgattg cggcgccaat ttcgttgttt ccatatacct tgctagccag tgccaacaca
     9181 tccttgacat cccaatcagt ctcctgcgca atggtcttct gtgcagccga agataacaca
     9241 aggtgcgatt caacgtccgg gatagtccgc aaagcctgta gcaggcggat tccatagatc
     9301 gcgccgctcg caccagatat gcccacaaca aaacgctcag ccatttttac ttgctccgcc
     9361 ggtcgacgag gcgacgcgcc ttgagctcga agcgcggcaa tgattcaggc ggggcaagcg
     9421 aaatagggac gcgcaagccc aaacgatgcg atgcatcacg ggcaatgcta ttggcaattg
     9481 cctgtgggtt ctcccccgcg atttccgctt tgatcgctag ttcatccatc gcttcaatgg
     9541 aatagacttc aaccgcaaac tcaatcacct cggggtacga gcgcataatc gcttcgatgg
     9601 cgctggggaa aatgttcacg ccgcgcacga tgagcatctg gtcgatgcgc ccaatgatcc
     9661 cgccatccat tcgcacaaaa gtccgtccgc attcacaggt agcccgattc agccgcacct
     9721 ggtcgccggt gcgataccgg atgatcggcg aaccgagacg acccaggttt gtgataatca
     9781 gttcgccctc gtcggcaggc atgcccgttt gcgggtcgat cgcttcaatt aaaaactcgc
     9841 cctcgttcag atgcacgccg ctttcggcaa ggcaagaaaa gccatgcgct cccacttctg
     9901 tcgcgccggt atggtcaaag agtttggcac cccatgcctc ttcaatgcgc gctttggtgc
     9961 cagggatact gccacctggt tcgccagcca caatgatgcg ccggacgcct gatgcccgca
    10021 aatcgacttt ttccttttgc gcgatttcaa ccaggtgcag agcgtagctg ggcgtgcaac
    10081 acagcgcggt cgcgcccagg tctatcaggt tgcgcaaacg ctggaccgaa tcctgcgctc
    10141 cgcccgagat ggccagcgcc ccaattttat cggagccggc ccacgccgcc caaaacccaa
    10201 tgaagggtcc aaatgaaaac gcgaaaaaga tgcggtcgct ttcactgatc cctgcggcgc
    10261 gatagactgc gctccagcaa cgcgcccacc aatcccagct atccggcgta tccagccaac
    10321 gcatcggctt tcccgttgtg cccgaagttt gatggatgtg cgtgtacctt tcaagcgggt
    10381 aggtgaggtt cgtcccgaac ggcggacgcg tttcctggtc gtctactaac tcctgtttac
    10441 gcgtgaaagg cagttgcgtg atgtcggcca gggatttgaa atcgcgcgcc tgcgtgaatc
    10501 ctgcttcgcg cagtttccga ccataaaacg cgtttgaggc aagcatctcg cccaggagca
    10561 cttgcattcg cccggtttgg tgtcctacca tctcttcccg gctggcggtt tcaatctcgc
    10621 ggttgaagta agttacttcc actggttctc ctcttttggc gtatgttttt attggcaggc
    10681 tgattgcgaa gaagatatcg caaataagcc gggggtttgg acgtatttga ggacgggcag
    10741 gaaatatcga ctgttcgtta ggtaactagc actgccagcg actctatttt gccggaattt
    10801 cccaattcca tgttagcaat gccaagcata gcgcctttcg tcatgttgga ataaattctc
    10861 gatgaggttt ttacgaaaag cgccaattcc cacgtcgttt tgcagttcat cctcataatt
    10921 tggataatat ttattatact actacatctt ggcataaatg agtctttcgt gcagggcaat
    10981 cgcatttggg cgatttccca ccacttagga gagcaaatgc catctctatg atcagagtgc
    11041 ttgtcagaaa aagcgcacag gtcagagtag ctcacattca tgtgtatctc gtttgagaaa
    11101 aaattcacaa tataaattgt ataggatatt acttttatca atttttgcag gataaatgtc
    11161 actaggatca gacgcgcgat attgctataa acattttgta ttcgctggga ttggcttgct
    11221 atcccagcaa caaccggtcc ctattatcta ttcatccaaa atctaaaacc aaaataaata
    11281 gcctgtttag tttccataaa gccaaaccat agcttccatc cagccaaagg ccaggggatt
    11341 gcccgaagtt ttgcagga
//
LOCUS       DBNGSX010000011         2487 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1385596, whole genome shotgun sequence.
ACCESSION   DBNGSX010000011 DBNGSX010000000
VERSION     DBNGSX010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1385596"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            586..909
                     /gene="trxA"
                     /locus_tag="AC16CB_00350"
     CDS             586..909
                     /gene="trxA"
                     /locus_tag="AC16CB_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR3340942.1"
                     /translation="MSDLAHVNEADFQQEVLEASQPVLVDFTAVWCAPCKMLDPVVKQ
                     LAQEWAGKVKVVKLDVDSNPNLAMHYQVMSVPTLMLFVNGEPSQRIFGYQPKDRIIAK
                     FGPSI"
     gene            1100..1540
                     /locus_tag="AC16CB_00355"
     CDS             1100..1540
                     /locus_tag="AC16CB_00355"
                     /inference="COORDINATES: protein motif:HMM:NF013629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR3340943.1"
                     /translation="MTHCDTLLQALRDLGYRITPQREMIVEAIAHTSGHATAEEIFEQ
                     VKTRTKYVNLATVYRTLDLLVERGLASRSDLWDGRVVYTTMHHGPHIHLVCRQCGGTY
                     DADHMLLQSLGEQLLASYDFTADLQHLSIIGLCADCRNKTKNAH"
     gene            1609..>2487
                     /locus_tag="AC16CB_00360"
     CDS             1609..>2487
                     /locus_tag="AC16CB_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HIR3340944.1"
                     /translation="MHYSPQPLHIPDGFLSVIVSLILWAISILVVGYALRRVGRDMGE
                     RQVPMMGVLAAAIFAGQMLNFSVAGGTSGHLMGAAIGTILLGPWAGILVMTCVVSIQA
                     LIFQDGGLLALGANIFNMGFIGVAVSYMAYRTIHKLAGGRRWGLFAGGFMAAWLSVEL
                     SALSVALQLALSGTSPANLAIPAMAGVHALIGIGEGLITVGALAFIAAARGEMLKAGE
                     SQPAGGVVWVFGLLAAIGLAVASPLASSNPDGLNWVAKRYGFMGLEQGPLYNLIPNYI
                     FPGISNGALASIAAGIL"
BASE COUNT          513 a          748 c          665 g          561 t
ORIGIN      
        1 aacacgctca tcaacggggc gctgcgcccc aggtaggcaa cacgccagtt ctccagggca
       61 ccctcatcat ccaccgcttc aagcgcatcg agtgcggcac gttgaatctc ttccaatttt
      121 tgtaacattc aaccctccaa gataatgaaa aactcccatc ccggcgaagg gacgggagca
      181 aattcccgcg gtaccaccca acttgatcgc ccaaggcgat ccaactcaga ctgacgagtt
      241 catcagctcc ccttttaacg ccggggtcgc ggcccaaact actaaacagt tcatgttcca
      301 agtgccgcac tatgtccaga acagtagaac ttggtacctg gaacgtcatt cacccgggca
      361 gctcaggagg gaacttcgga cagtttccac cgggctgggg tctcagtctg cgccccatgc
      421 ctccctgtcg gcttcagcta gcctactttc ctccgtccaa gctattgcag gtcggttgtt
      481 gcccctatta tcttcaaaaa gaaggttctg tcaagatggg gacttccttg acttttcggg
      541 cgagtatgat taccatgtct cccaatagtg catggaggta attcgatgag cgatttggct
      601 cacgtgaatg aagccgattt tcaacaggag gtgttggaag ccagtcaacc cgtgctggtg
      661 gatttcacgg cggtatggtg cgcgccgtgc aagatgctcg acccggtggt caagcagctt
      721 gcccaggagt gggctggcaa agtcaaagtg gtcaagctgg atgtggacag caaccccaac
      781 ctggcaatgc actaccaggt gatgagtgta cccacgctga tgctgtttgt taacggggag
      841 cccagccagc gcattttcgg ctaccagccc aaggatcgca ttatcgccaa gttcggtccg
      901 tcgatttgac caaataatct atccccattt ttagcgagaa aaaaggtgtt tgcataggca
      961 gatacctttt tttatgccac aacacccctg tcctgtagca agggcttgga tttattatga
     1021 ccacatgaca agtcctttac aatctctacc taatccggta taatcttatt gcaaatattc
     1081 gcaactgaaa gttttcgcaa tgacccactg tgacacccta ctccaagccc tgcgtgatct
     1141 gggctaccgc atcacccccc agcgtgagat gatcgtcgag gccatcgccc ataccagcgg
     1201 tcacgccact gccgaagaga tcttcgagca ggtcaaaacg cgcaccaaat acgtcaacct
     1261 ggccacagtt taccgcaccc tagacttgct ggttgagaga ggcctcgcca gccgctcaga
     1321 cctatgggat ggccgcgtgg tgtacaccac catgcaccac ggaccgcata tccacctggt
     1381 ctgccgccag tgcggaggca cctatgatgc cgaccacatg ctgctccaat ctcttggcga
     1441 gcaattgctc gccagctatg actttaccgc agacctgcag cacctgtcta tcatcgggct
     1501 gtgcgctgac tgccgcaata aaaccaagaa cgcgcattga cctcttcaac aaccgccgcc
     1561 atactcgcca ggcggggttg tatgcgcttt tctccctagg aggtacccat gcactactca
     1621 cctcaacctt tgcatatccc tgacggtttt ctatcagtga tcgtctcatt aattctctgg
     1681 gctatctcga tccttgtggt cggttacgca ctgcgacgcg ttgggcgcga tatgggcgag
     1741 cgccaggtgc ccatgatggg tgtgctagcc gcggctatct tcgccgggca gatgctcaat
     1801 ttcagcgtgg cgggcggcac gtccggtcac ctgatgggcg cagcgattgg gaccatcttg
     1861 ctaggcccct gggcgggcat cctggtgatg acctgcgtgg tgagcatcca agccttgatc
     1921 ttccaggatg gcggcctgct tgcgctggga gctaatattt tcaacatggg gttcatcggt
     1981 gtggcggtct cctatatggc ttaccgcacc atccacaagc tggcgggcgg gcgccgctgg
     2041 gggttgttcg ctggcgggtt catggcggcc tggctatctg ttgagctatc ggccttgagc
     2101 gtagccctgc aacttgccct ctccgggaca tccccggcta atctcgccat cccagccatg
     2161 gcgggcgtgc acgctctcat cggcatcggc gagggattga tcaccgttgg cgcgctggcg
     2221 tttatcgccg ccgcacgggg ggagatgctc aaggccggag aatcacagcc agccgggggc
     2281 gtagtgtggg tcttcggctt actagctgcc atcggcttag cggttgcctc cccgctggcg
     2341 tccagcaacc cagacgggct gaattgggtg gccaagcgat atggcttcat gggactggaa
     2401 caaggcccgc tgtacaacct gatccccaat tacatcttcc ccggcatctc caatggcgcc
     2461 ctggcaagca tcgccgcggg catcctg
//
LOCUS       DBNGSX010000012         3031 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1039341, whole genome shotgun sequence.
ACCESSION   DBNGSX010000012 DBNGSX010000000
VERSION     DBNGSX010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1039341"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..2817
                     /locus_tag="AC16CB_00365"
     CDS             286..2817
                     /locus_tag="AC16CB_00365"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase family protein"
                     /protein_id="HIR3340945.1"
                     /translation="MVMKNAIQNHLPHAFKPNSRVGFALILLSTATLAFEINLTRLFS
                     VAQFYHFAFMVVSLALLGFGASGTFLAIFPLDTKHSMDRVMGNLALGASATMLASFLL
                     SNWIPFDSFSIAWDRRQVAILILQYLALVAPFFFCGLAISILLTTYNQEVGKVYAINL
                     LGSSVGCLCALVVPNFLGGVGMVSLASALAGIAALFCWLPAFVVKSVKKPKSVVKNLW
                     SNSLNALARILLLALLSFTLGDTFLRLAGMEGFPAMEIRISPYKSLSYALQYPDSQVS
                     YRRWNAYSRVDLVRSSGIRSLPGLSYRYLQPPPPQDGLLVDGDDPSPVVINPENPGFA
                     EYLPASLAFHLRPDAQTLILEPRGGVDILTALAGGARQITAVEVNPLIVEAASQIYGS
                     PKVQTVIDSGRSYLRRTDQQFDVIILSLISSYHPVRSGAYSLAEDYRYTQEAFQDMLD
                     HLKPGGLLVLMRWLQTPPSESLRVFALASTALEDRDADPKSQIVALRGYNAATLLIQN
                     GPFTNAEIDTVREFAASRAFDLDYFPGIAPQDVNQYNVLPEALDYQAYTDLLDSPTPQ
                     TFYRNYPFDISPPTDDHPFFSHSFKWEQADEVIAEMGKTWQPFGGAGYFVVIALLLLA
                     LIGSSLLIVLPLAIHRRNMPLTEKSSGLSKVRLRSLLYFGLIGIGFLFVEIPLIQKFI
                     LFLGNPAYAMTTVLFTLLLFSGLGSQFSHRIPIRLGIGALVILVVILPYFLPLLFRET
                     LGFSIAAKLLISCLVLSPVGFLMGIPFPAGLRLISRLVHSEAWIAWGWAVNGASSVMA
                     SILAALFALSWGFSWVFYLGAACYAGAWLTGSAMASLPVLPPPDR"
     gene            complement(2765..>3031)
                     /locus_tag="AC16CB_00370"
     CDS             complement(2765..>3031)
                     /locus_tag="AC16CB_00370"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase
                     family protein"
                     /protein_id="HIR3340946.1"
                     /translation="YYGWPEYAPFDAIIVTAAPDHLPSPLAAQLAENGRLVIPIGPPG
                     YFQSLWKFYLEDNQLKAVNLGGVAFVPFTGPGVEAQAETPSLIP"
BASE COUNT          676 a          795 c          706 g          854 t
ORIGIN      
        1 cgatgaacaa caccagaatg gtaatcagtg cgataaccat gctgaacaat aagtatcgcc
       61 cacgcaagcg ccaaatttct ttcaatgcta ggtaaaagga cattttccct ccgatgctac
      121 gcaattatac caaattagat ttaattacgc tcatttacat tgtacacata ttgtacagtt
      181 ttgtcaatgg ttttgtaaaa gattctcttt tctaacagca ttttccctgt ttaccttata
      241 cattttctct cccgccaagc agtataataa tcttgggaga catagatggt catgaaaaat
      301 gcaatacaaa atcatctccc tcacgccttt aaaccaaatt ctcgggtcgg ttttgccctc
      361 attttgttat ccacagccac tctggctttc gaaatcaatc tgacacgtct tttctcggtc
      421 gcccaattct accatttcgc tttcatggtc gttagcctgg cattgttggg ctttggtgcc
      481 agtggcactt tcttggctat ttttccgctg gatactaaac atagcatgga tcgagtgatg
      541 ggcaatcttg ccctgggcgc atccgcaacc atgctcgcat catttctcct atcgaattgg
      601 ataccatttg actcgttcag catcgcctgg gatcgacgtc aagtcgcgat attgatactg
      661 caatatcttg cactagtggc accatttttc ttttgcggtc tagccatcag tatcttgctc
      721 accacataca atcaagaagt cgggaaagtc tatgccatta atttactggg atcctccgtc
      781 ggttgcctat gcgcactcgt tgtgccgaat ttcttgggcg gtgtggggat ggtttcgtta
      841 gccagcgcac ttgctggcat cgcggcactc ttttgctggc tgcccgcttt cgtggttaag
      901 tcggtgaaaa agcctaagtc ggtagttaag aatctgtgga gcaattctct aaatgccctg
      961 gcacgaattc tgctgctcgc ccttctgagc ttcacattgg gcgatacctt cttgaggctg
     1021 gcaggcatgg aaggttttcc agcaatggag attcgcattt ccccatataa aagcctctct
     1081 tatgccctac aatatcccga ttctcaggtc agctaccgcc gctggaatgc atactcgcgt
     1141 gtggacctag ttcgcagcag cggtattcgc tccctgcctg gcttgagcta tcgctacctc
     1201 caaccccctc ccccacaaga tggtttgttg gttgatggcg atgatcccag tcccgtggtg
     1261 atcaatcccg aaaatccagg attcgctgaa tatctgccgg cgtccctggc gtttcacctg
     1321 cgtccggatg cacaaaccct cattttggag ccacgcggtg gtgtggatat cctgactgcc
     1381 ctggctggcg gggcgcggca gattaccgcc gttgaggtca atccgctgat tgtagaagca
     1441 gctagtcaaa tttatgggtc tccaaaagta caaactgtca ttgattccgg gcgcagctac
     1501 cttcgtagga cagaccagca attcgatgtc atcattctct ccctgattag ctcgtaccat
     1561 ccggttcgct ccggtgcgta cagcctggct gaagactatc gctatacaca agaggccttc
     1621 caggatatgc tggaccacct gaagccaggt ggcttgctcg tgctcatgcg ttggctgcaa
     1681 actcctccta gtgaaagcct tagggtcttc gccctggcat ccactgccct ggaagatcgt
     1741 gatgcagacc cgaaaagcca aatcgtagcc ttgcggggtt ataacgcggc taccctgtta
     1801 attcaaaatg gtccatttac aaacgccgag atagataccg tgcgtgaatt cgccgcctcg
     1861 cgcgccttcg acctggacta tttcccaggg atcgcaccac aagatgtgaa tcaatacaat
     1921 gtgcttcccg aagcgttgga ttatcaggca tatacggacc tattggactc ccccactcca
     1981 caaacttttt accgcaatta cccttttgac atcagccccc cgacagacga tcatcccttc
     2041 ttcagtcatt cctttaaatg ggagcaggca gacgaggtaa ttgctgaaat gggaaaaacc
     2101 tggcagcctt ttggcggggc aggatatttt gtggttattg ccttgttatt gttagcttta
     2161 atcggctcca gcttgttaat cgtcttaccc ttagcgattc accggcgaaa catgccactc
     2221 acagaaaaat caagtggatt atcaaaggtt aggcttcgat cattactata ttttgggttg
     2281 atcggtatcg gcttcttgtt tgtggaaatc ccgttgatcc aaaagtttat cttgttctta
     2341 ggcaacccgg catacgccat gaccacagtc ctcttcacat tattattgtt ctctggcttg
     2401 ggtagtcaat tcagccacag aatcccaatc cgtcttggga taggcgcact ggtgatcttg
     2461 gtcgtcattc ttccctactt tttgcccctg ctattcagag agacactggg cttttcgatc
     2521 gcagcgaaac tgctcatcag ttgtctcgta ttatcaccag tgggatttct gatgggcatc
     2581 cctttccctg ccggattacg actgatctca agattggttc acagcgaggc atggattgcc
     2641 tggggatggg ctgtaaatgg cgcatcttcg gttatggcgt ccattctggc agccttattt
     2701 gcgctctctt ggggttttag ctgggtattc tatctgggtg cagcttgcta cgcaggtgcg
     2761 tggcttacgg gatcagcgat ggcgtctctg cctgtgcttc cacccccgga ccggtgaacg
     2821 gaacgaacgc taccccaccc agattaactg ccttcaactg gttatcttct aaatagaatt
     2881 tccacagcga ctgaaaatat cccggcgggc cgatagggat gaccagacgc ccattctcag
     2941 ccagttgggc tgctaatggg gagggcaggt ggtcgggagc tgcagtgacg ataatggcat
     3001 caaatggggc atattcaggc cagccataat a
//
LOCUS       DBNGSX010000013         3292 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_485388, whole genome shotgun sequence.
ACCESSION   DBNGSX010000013 DBNGSX010000000
VERSION     DBNGSX010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_485388"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="AC16CB_00375"
     CDS             <1..308
                     /locus_tag="AC16CB_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004101778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR3340947.1"
                     /translation="MSNRLAVMYLGKIVEMGDTEEVLAKPLHPYTKALLSAVPVPDPT
                     YARTIPEIKGSIAQPINPLPRCRFYDRCPIAAKVCEQNDHPPLLDKGSRHQVACYLV"
     gene            complement(312..1748)
                     /locus_tag="AC16CB_00380"
     CDS             complement(312..1748)
                     /locus_tag="AC16CB_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3340948.1"
                     /translation="MASATQFQEDLKAYRLRQNRLRIWLSLQWRQARETWGLFSKNRL
                     AVFGVALIFIYALMSITHPILMSTVWSKGVYDPHVGYDLEIFPHPSPPGLGHPLGTDT
                     LGRDVLSRLLAATTPTFTLALTAALTTAIFSTLFAAFSAYAGGLIQSIQTHISDIFLL
                     LPAPLVMIIIGGTIDINPLVFGLIYGLIAGLGGAAIVMRSQALTIIRKPYIEAALVSG
                     ASGVQIIFKHIIPNMLPLAAVQMLISVTGAVFADGFSTFLGISRTRLNWGSMIYDSFT
                     YQAINPAITWNVLIPSALAISLFATAFYLIARGIHEVAEPRLRDWEAAPTIKKLLAEQ
                     LEQAKSPAKPIPPPSLDDTAPVRVAPRSRIVLDDQAAFAWLEYLAVSQGANEALLLRP
                     EERRSKPPEWVTQSNIGVPPPLQSEAKIKVDDPPIFQPAPSSDKSLSIHEQARQAIEQ
                     GDAYLRAGELQQALDAYIKAEKLIRDGK"
     gene            complement(1748..2914)
                     /locus_tag="AC16CB_00385"
     CDS             complement(1748..2914)
                     /locus_tag="AC16CB_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3340949.1"
                     /translation="MLRFIIKRFLSGLVVLFLFQTAIFFAVQLILPGDFASHFALMLS
                     RDEIQELRQILGLDLPIWERYLNWLQNMVRGDMGRSFTLRGSGPTVIEVLKAVTPPTI
                     LVFGIGTVLAFLLGLWLGKVTGWRRPGLITGSITFGGIALYTSFPPWLAFLLVYFLST
                     QLGLLPRSFTNKLWQGAPTSISQIMLQMVFALLVVLAILLLIQWLVRRITRRSMPTIV
                     LLLLTAAGWYASWYWMDILPYAVDIARAAALPLVGYVLLSFGEIMLLMRTTMLDVMHE
                     QYVHTARAKGLTSNDVRDRHVARNAILPVMSGLVVRLPYLLTGAVMLERALNWEGVGT
                     ELFTAVGLQNILLVMGLILIIGAISLVARLLLDILQALLDPRLRTAGGELGGTL"
     gene            complement(3087..>3292)
                     /locus_tag="AC16CB_00390"
     CDS             complement(3087..>3292)
                     /locus_tag="AC16CB_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340950.1"
                     /translation="ITVIGFTLPWAISALWSLNNFPDINRVHFFGARSNTLFAGLRMP
                     FRAYAIALMYLTKFTRNPRGALP"
BASE COUNT          796 a          859 c          927 g          710 t
ORIGIN      
        1 atatgtcgaa ccgcctggca gtaatgtatc tgggtaagat cgtggagatg ggtgatacag
       61 aagaagtgct ggcaaagccg cttcacccat ataccaaggc gctgctctcc gctgtgccgg
      121 ttcctgaccc aacctatgcg cgtaccatcc cagagattaa gggtagcatt gcccagccga
      181 tcaatccctt accgcgctgc cggttctacg accgctgccc gatagctgcc aaagtgtgtg
      241 agcaaaacga ccacccaccc ttgcttgata agggtagcag acaccaggtg gcctgctact
      301 tggtgtaaat attacttccc atctcgaatg agtttttctg ccttaatgta ggcgtctagc
      361 gcttgctgta gttcccctgc gcgcaggtat gcatcgcctt gttcgattgc ctggcgtgcc
      421 tgttcgtgga tggagaggct tttatcggag ctgggggctg gctggaaaat gggggggtca
      481 tcaaccttta ttttcgcctc agattgtagc gggggtggaa caccaatgtt agattgagtt
      541 acccattctg ggggtttgct gcggcgttcc tcaggcctga gcagcaatgc ttcgttggct
      601 ccctgactca ccgccaggta ttccagccag gcaaatgcgg cttgatcgtc tagaacaata
      661 cgactccgag gggcgacacg cacaggtgcg gtatcatcca gggatggtgg cgggataggc
      721 ttggctggcg atttagcctg ctctagctgc tctgcaagca gtttttttat ggtgggtgcg
      781 gcttcccaat ccctcaggcg tggttcagcc acttcgtgga tgccgcgggc gatcaaatag
      841 aaagccgtcg caaacaatga gattgccagt gcggaaggta tcaacacgtt ccaggtgatg
      901 gcgggattga ttgcctggta ggtgaaactg tcatagatca tgctgcccca gttgagcctg
      961 gtgcgtgata tccccaggaa agtgctgaag ccgtcggcga agactgcccc ggtgacactg
     1021 atcagcatct gtaccgcagc cagcgggagc atattcggga tgatgtgctt gaagatgatc
     1081 tgtaccccac tagccccgct gactagggca gcctcaatgt agggctttcg gatgatggtg
     1141 agcgcctgag agcgcatcac gatcgccgcg ccccccagcc cggcaattag cccgtatatt
     1201 aaaccaaaga caagcgggtt gatgtctatt gtgccaccaa tgatgatcat gaccagcggg
     1261 gcaggtagta ataagaagat atccgaaata tgcgtttgga tggactggat taagccaccg
     1321 gcgtaggcag agaaggccgc gaagagtgtg ctgaagatag ctgttgtcag ggcggcggtg
     1381 agtgccaggg tgaaagtagg tgtggtggct gcaagtagcc ggctgagcac atcgcgtccc
     1441 aatgtgtcgg tgcccagtgg gtgtccgaga cccggcggtg aggggtgggg gaagatttcg
     1501 agatcgtagc ccacatgcgg atcgtatacg cccttcgacc ataccgtgga catcaggatg
     1561 ggatgggtga tggacatcag tgcatagatg aaaattaacg ctaccccgaa tactgctaac
     1621 cggttttttg agaacaagcc ccatgtctcg cgtgcctgtc tccactgcag cgaaagccat
     1681 attctcaagc gattctgccg caggcgatag gctttcaaat cttcctggaa ttgtgtggcg
     1741 gaggccatta aagcgtcccc cccagctcac cacctgcagt acgcaggcgt gggtcgagca
     1801 aagcttgaag gatatccagc agcagtcgcg ccaccagtga gatcgcgccg atgatcaaaa
     1861 tcaagcccat gaccaataag atgttctgta gcccgaccgc cgtgaacagc tctgtgccaa
     1921 ctccctccca gttgagcgct ctttcgagca tgacagcccc cgtgagcagg taaggcagac
     1981 ggacgaccag accgctcatc acaggcaata tcgcgttgcg ggctacgtgg cgatcccgca
     2041 catcattgct ggttaaaccc ttggcgcgcg ctgtgtgaac gtattgctcg tgcatcacgt
     2101 cgagcatagt tgtgcgcatc agcagcatga tctcgccgaa tgaaagtaac acatacccga
     2161 ccaggggcag agcggctgcc ctggcgatat ccactgcata gggaagtata tccatccagt
     2221 accagctagc gtaccatccc gcagctgtca gcaagagcaa aacgattgtg ggcatcgacc
     2281 ggcgagtgat gcgacgaaca agccattgga tcagtagcag tatcgccagc acgacaagta
     2341 gggcgaagac catctgcagc atgatctggc tgatgctcgt tggggcaccc tgccatagtt
     2401 tgttggtgaa cgacctgggc agcaagccta attgggtaga caggaaatag accagcagga
     2461 acgccagcca gggtgggaac gaggtgtaca aggcgattcc gccaaaagtg atcgagccgg
     2521 tgattagtcc gggtcggcgc cagccagtga cctttcccag ccacagtccc agcaagaagg
     2581 ctagcacagt gccaatgccg aaaaccagga tagtcggcgg ggtaaccgct tttaggactt
     2641 ctataactgt cggaccagag cctctcaagg tgaaggaacg tcccatatcg cctctcacca
     2701 tattctgcag ccaattgaga tagcgctccc agatgggtag atctagccca agtatctgac
     2761 gcagctcttg tatttcatcg cgagatagca tcaaagcaaa atggctggca aaatcgccag
     2821 ggaggatcag ctgcacggca aaaaagatcg cagtctggaa caagaacaag acaaccagac
     2881 cggacaagaa tcttttaatt atgaaacgca gcatgatgga ccctttgcaa ctttggtgac
     2941 cccacccctt tggatattca cgatcttcag agcctataaa tgccttctgt ctctaaataa
     3001 taccatgtga tcactgcttt agttcatcat atctcctttc cttggatctg ccgttaccga
     3061 ccagcacttc tggagtctcg accaaactag ggcagcgcac cacgaggatt cctggtgaat
     3121 ttggtgagat acatcagggc aattgcatat gcccggaagg gcatgcgcaa tcccgcaaaa
     3181 agagtgtttg accttgcccc gaaaaagtgg acacgattaa tgtccgggaa attgttcaaa
     3241 ctccacaggg ctgagatagc ccaaggaaga gtgaagccga tgacggttat ac
//
LOCUS       DBNGSX010000014         3980 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_139087, whole genome shotgun sequence.
ACCESSION   DBNGSX010000014 DBNGSX010000000
VERSION     DBNGSX010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_139087"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1960)
                     /gene="acnA"
                     /locus_tag="AC16CB_00395"
     CDS             complement(<1..1960)
                     /gene="acnA"
                     /locus_tag="AC16CB_00395"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HIR3340951.1"
                     /translation="MHKDYFNARAVLKTSHGDYIFFHLGRLEKEGLTQLERLPFSIRV
                     MLESLLRQCNEREITRQDVINLAGWTAQAAERPTLPYRPARVIMQDLTGVPAVVDLAA
                     MRSAVSRLGGDPKQINPLVPVDLVIDHSVQVDFFATPDALMRNADLEFQRNQERYEFL
                     RWGQQAFDNFRLVPPATGIVHQVNLEYLADVVMANDEDGERIAYPDTLVGTDSHTTMI
                     NGLGVVGWGVGGIEAEAAMLGQPIDMLTPDVVGFKMEGQLNEGVTATDLVLTVTQMLR
                     KKGVVDKFVEFFGSGLDSMSLTDRATIANMAPEYGATIGFFPVDDETMRYLRLTGRSP
                     EVIERVERYTKEQGLFRTTHTPDPEFTDTLELDLSSVEPSLAGPKRPQDRIPLREMKR
                     AFRSALTAPVKERGYELEPEALERSAAMGTNGGSVEMGHGSVVIAAITSCTNTSNPAV
                     MIGAGLVAKKAVERGLQVRPYVKTSLAPGSRVVTDYLSKAGLLVSLEELGFNVVGYGC
                     TTCIGNSGPLSGEVVKAISGTDLVAAAVLSGNRNFEGRVHPFVRANYLASPPLVVAYA
                     LAGTVDIDLVNDPLGVDRDDQPVYLRDIWPSTQEIQDTIAQAVLPEMFAEKYADVFSG
                     TQTWKDIQASQGELFEWNPSSTYIQEPPF"
     gene            complement(2020..2913)
                     /gene="folP"
                     /locus_tag="AC16CB_00400"
     CDS             complement(2020..2913)
                     /gene="folP"
                     /locus_tag="AC16CB_00400"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006093086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR3340952.1"
                     /translation="MHNSLTIGNSTFTWGARTYVMGILNITPDSFSGDGLLSSISDTG
                     DKWLQTALELARGYVADGADLLDVGGESTRPGAETVSLEEELERVLPVIRQLASEIDV
                     PISIDTYKAEVAEAALEAGAHMVNDVWGFKVDPRLAEVVARHKVPVILMHNRSSWANA
                     EIQARLGGRYVGVPYENLLEDIRRELMESVAIARSAGIPDEHIILDPGIGFGKTVEQN
                     LELLDRLGEIRALGFPIMLGPSRKSFIGYTLNLPPDQRVEGTAAAVAIGITRGADIVR
                     VHDVKSMARVTRMGDAIVRRG"
     gene            complement(2913..3338)
                     /locus_tag="AC16CB_00405"
     CDS             complement(2913..3338)
                     /locus_tag="AC16CB_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340953.1"
                     /translation="MSEEKLKPLKWKPDPDVGYTVVRRQDGGMHITFTDVSHTTLEHW
                     REFAMEHLLNSDRLTRNLYDLRQIDELPEEAVQYAIEVNNDPSVRNIRLAVVATHDSV
                     KEALKRIAALTTSGGVEMGLFSTLEDADAWLNRPLTLLV"
     gene            complement(3483..>3980)
                     /gene="folK"
                     /locus_tag="AC16CB_00410"
     CDS             complement(3483..>3980)
                     /gene="folK"
                     /locus_tag="AC16CB_00410"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016330205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIR3340954.1"
                     /translation="VNKTHSAFVALGSNLGDRLGNLQAAIGLMPPALHVKEQSPIYET
                     SPWGYADQPDFLNQVVHAETDLAPLELLAYIKSIEVTLGRTPTFVYGPRQIDLDILFY
                     DDLVLQTQELIIPHPHLASRAFVLCPLADIAPDLRHPIQGQTVLQLLEGVDRQGIRLL
                     RANAS"
BASE COUNT          918 a         1104 c         1084 g          874 t
ORIGIN      
        1 agaatggtgg ctcctgaata taggtagatg acggattcca ctcgaacaat tcgccctggc
       61 tggcttgtat atctttccaa gtctgggtac cgctgaagac atcggcgtat ttctcggcga
      121 acatctcggg cagcacagcc tgtgcgatgg tgtcctgtat ctcctgtgtc gatggccaaa
      181 tatcgcgcag atacaccggc tggtcatcac ggtcaacacc caaaggatca ttgaccagat
      241 caatgtccac cgtccccgcc agggcatagg cgaccaccaa tgggggagag gcgagatagt
      301 tggcacgcac gaaggggtgt acccgtcctt caaagttgcg gtttcccgag agcaccgctg
      361 cagcaaccag atcagtgccg gaaatcgcct tgaccacctc gcctgagagc ggacccgagt
      421 ttccaataca agtggtacag ccatatccga cgacattgaa gcccagctcc tccagcgaga
      481 cgagcaggcc ggctttgctt agataatcgg tcaccacgcg cgagccaggc gccaggctgg
      541 tcttgacata gggcctgacc tgcaggccgc gctcgacagc cttttttgct accaggccgg
      601 ctccgatcat caccgctgga ttggaggtgt tggtgcaaga ggtgatagca gcgatgacga
      661 cagaaccatg ccccatctct acactacccc cgttggtgcc cattgccgct gagcgttcta
      721 gtgcttctgg ttccagctcg tagccacgct ctttgactgg ggcggtgagt gcgctacgaa
      781 atgccctctt catctctcta agcgggatgc gatcttgagg gcgcttgggg ccggcaaggc
      841 tgggttctac actgctcagg tcgagctcca acgtatcggt gaactcagga tcaggtgtgt
      901 gggtggtgcg aaacagcccc tgctctttgg tatagcgctc gacgcgctca attacctcgg
      961 gggatcgccc ggtcaggcgc aaatagcgca ttgtctcatc atccacaggg aagaagccga
     1021 tggtggcgcc gtattcggga gccatattgg cgatggtggc acgatccgtc agagacatcg
     1081 agtccagccc agagccgaag aactccacga atttatccac aacccctttc ttgcgcagca
     1141 tctgggtgac agtcagcacc aggtcggtgg cggtaacccc ttcattgagt tggccttcca
     1201 tcttaaatcc gaccacatct ggcgtcagca tatcaatcgg ctggcctagc atagcagcct
     1261 cggcttcgat gccgcctaca ccccagccga ccacacccag gccattgatc atggtggtgt
     1321 gcgagtcggt accgaccagt gtgtccgggt aggcaatcct ctcaccatcc tcatcatttg
     1381 ccatgaccac atctgccagg tattccaggt taacctggtg gacgataccg gtggcaggtg
     1441 gcaccaagcg aaagttatca aaggcttgtt gtccccagcg cagaaattca tagcgttctt
     1501 gattgcgctg gaattccaaa tcagcgttgc gcatcagcgc gtccggtgtg gcaaaaaagt
     1561 ccacttgaac ggaatggtcg atgaccaaat caaccgggac gaggggattg atctgtttgg
     1621 gatcaccacc caggcgcgac acagccgagc gcatagctgc taggtcgaca acggctggga
     1681 caccggtcag gtcttgcata attacacggg cggggcggta gggcagcgtg ggccgctctg
     1741 cggcttgtgc cgtccagcca gccagattga tcacatcttg gcgagtgatc tcacgctcgt
     1801 tgcattgcct tagcagagat tcgagcataa cacggatgga aaaaggcaag cgctccaatt
     1861 gggtcaaacc ttccttttcc agacgaccca ggtggaaaaa gatgtagtca ccgtgagacg
     1921 ttttgaggac cgcgcgagca ttgaaataat ctttgtgcat gaacatttcc ttgcaagagg
     1981 atatttgaaa aagctaattg gggattgaga ttataccatc tatccccgtc tgacgatggc
     2041 gtcccccatt cttgtcacac gagccataga tttcacgtcg tggacgcgga caatatccgc
     2101 cccacgggta ataccgattg cgacggctgc ggcggtaccc tccacacgct gatcaggggg
     2161 aagattcaag gtgtagccga tgaacgactt gcgcgatggc cccagcatta tagggaagcc
     2221 aagtgcgcgg atttccccca agcggtccaa aagctccaga ttctgctcca cagtcttccc
     2281 aaagccaatc ccagggtcaa ggataatgtg ctcgtctggt attccagccg acctggcgat
     2341 agctacgctt tccatcagct cgcgccggat atcttccagc aagttctcgt aaggaacacc
     2401 cacataacgc ccccccagtc gggcttgaat ctcagcattt gcccacgagg agcggttgtg
     2461 catgaggatg acgggcacct tgtgacgggc tacgacttcg gccaggcgcg ggtccacttt
     2521 gaacccccag acatcattga ccatgtgcgc tcctgcttcc agggcagcct ccgccacctc
     2581 agccttgtag gtgtcaatcg agatgggaac atcgatctcc gatgccagct ggcggatgac
     2641 cggcagaacg cgctccaatt cctcttcaag gcttacagtt tccgcgccag gacgcgtgct
     2701 ctcaccgccc acatcgagaa ggtcagctcc atcagcgacg tagccgcgag cgagctccag
     2761 ggcagtctgt agccacttat cccctgtatc agatattgag gaaagcagcc catcgcctga
     2821 aaaactgtca ggcgtgatat tgaggatacc catgacgtag gtccgggctc cccatgtaaa
     2881 agttgaattt cctatggtga gagaattatg cattaaacca gtagcgtgag aggacggttt
     2941 agccaggcat ctgcatcttc aagggtgctg aataggccca tctcaacgcc accggatgtg
     3001 gtcaaagctg caattcgctt gagcgcttct ttcacagaat catgggtggc tacgaccgcc
     3061 aagcggatat tgcgcaccga ggggtcgttg ttcacctcaa tcgcatattg aacagcttct
     3121 tcaggcaact cgtcgatctg gcgaagatcg tagaggttac gtgtcaggcg gtcggagttg
     3181 agcaagtgct ccatagcaaa ctcgcgccag tgttccagtg tagtgtgcga gacatcggta
     3241 aaagtaatgt gcataccgcc atcctgccgg cggacaaccg tatagcccac atcaggatcg
     3301 ggtttccact tgaggggctt taatttttct tcgctcatga cttgcctcca caactcggtt
     3361 atgaattgaa tattaaccga gaaaagatct cccatgcgag tcgtaaagat attctatcct
     3421 aaattttgtc caagtatatg ataagaaaat gaattcaaac ggacacatgt cacatttctt
     3481 gctcaggagg cattcgctcg cagcagtcga atgccctggc gatccacgcc ttccagtagt
     3541 tgcaacaccg tttgcccttg tatagggtgg cgcaagtctg gagcaatgtc agccaggggg
     3601 cagagtacaa aagcgcgcga tgccaggtga ggatggggga ttatcagctc ctgggtttgc
     3661 agcaccaggt catcgtaaaa caggatatcc aggtcgatct ggcgcggtcc ataaacgaag
     3721 gtaggtgtgc gtcccagggt aacctcgatg gacttaatgt aagccaacaa ttctaagggc
     3781 gctagatcgg tctcggcgtg aacaacctgg ttgaggaaat ccggctgatc tgcatagccc
     3841 cacggggatg tctcatagat aggcgattgc tccttcacat gcaaagctgg aggcatcagc
     3901 cctatagctg cttgcaaatt tcccagccgg tcacccaggt tgctacccag cgcgacgaag
     3961 gcggaatgtg ttttattcac
//
LOCUS       DBNGSX010000015         5744 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_208447, whole genome shotgun sequence.
ACCESSION   DBNGSX010000015 DBNGSX010000000
VERSION     DBNGSX010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_208447"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..549
                     /gene="ssrA"
                     /locus_tag="AC16CB_00415"
     tmRNA           151..549
                     /gene="ssrA"
                     /locus_tag="AC16CB_00415"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(595..1803)
                     /locus_tag="AC16CB_00420"
     CDS             complement(595..1803)
                     /locus_tag="AC16CB_00420"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014895731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HIR3340955.1"
                     /translation="MNEVILKSGREKSVLRQHPWVYSGAIHRVVGNPTLGETVLLKDF
                     LGQPLALGAYSPQSNIRIRIWTWNLDESIDREFFQRRLQEAVSLRQTLYNLRIADEVE
                     TDAMRLVHAESDNLPGLVVDRYGDTLVVQYLTAGVELWREILTDILMEITQVERIYER
                     SDVNVRELEGLPKRAGLLRGSAPPERVLIQEGKLSFWVDITKGHKTGFYLDQRPNRFK
                     IAQMARDREVLDCFTYTGGFALSSLTGGAKAVTALDVSADAVALAKANLAINRLSTDR
                     VEWIEGDVFQTLRKFRDQAREFDLIILDPPKFAHTTAQVERASRGYKDINLLALKLLR
                     PGGLLATFSCSGGVSEDLFQKIIFGAALDAGVEAKIIERLHQGFDHPVALNFPEGAYL
                     KGFILSVSKS"
     gene            complement(1800..3011)
                     /locus_tag="AC16CB_00425"
     CDS             complement(1800..3011)
                     /locus_tag="AC16CB_00425"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3340956.1"
                     /translation="MGQRINDAITERIQAMNERVNITKELGLYFYNQPIKELRGGARV
                     VVNGREMGMYASYSYLGLIGHPRINAAAKAAIDQYGTGTHGVRSLAGSLTLHKELEEK
                     IANFKGAEAAITFSSGYVTNLTVVSTLIGRGSYVISDKLNHASIVDGCLMSGAKFLRF
                     RHNDMESLEQRLQQAESDGGATKLVIADSVFSMDGDIIDFPKMVELCRNYNAWLMIDE
                     AHSVGVLGKNGRGIEEHFDMPGSIDIKMGTLSKTIPSVGGYVAGNRDLIQYLRHASRA
                     YIFSAALPPAQAAAANAAFDVILDEPWRVTKLNANTRQFINGLKERGFDTMYTQTAIV
                     PVLCGSDESAFGVTAEAQHNDLFVLPVVSPAVPPGLARLRATITAAHEMDEIEHAMDI
                     IEDAGKKVGII"
     gene            complement(3029..5068)
                     /gene="ligA"
                     /locus_tag="AC16CB_00430"
     CDS             complement(3029..5068)
                     /gene="ligA"
                     /locus_tag="AC16CB_00430"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR3340957.1"
                     /translation="MNISDVDQSLRDRIENLRREIHYHNYRYHVLDSPLISDYEFDQL
                     MLELRQIEEEHPDWITTDSPTQRAGSVPSEKFIKVRHPAPILSLGNAFDADGVRAWFD
                     RILKLDERVIQADFVVEPKIDGLTVVLHYRNGIFVQGATRGDGEIGEDITANLRTIRA
                     LPLRIPISVDGPMPPSYLVVRGEAFITPADFEALNRQLEERGEKTYQNPRNTAAGALR
                     QLDPTLTATRPLTLLTYDVVDGDGSLSESHTETLGMLRALGFPVIDEEHCDSLDDAIQ
                     AYNRLVERRDQLPFEADGVVIKINDLGLGENLGVVGKDPRGAIAFKFPAREVTTTLMG
                     IGVNVGRTGVLTPYAILEPVEIGGVIVRQATLHNFDYIADKDIRIGDRVAVKRAGDVI
                     PYVIGPLTTARTGQEREFIPPEVCPACEQPVEHLEGEVAWYCVNSACPAQLIRNLEHF
                     VSRGAMDIVGLGIRIVEQLVDSGFIKDVADLYSLKHEDLLGLEGFAEKKADNLLQSIE
                     LSKNQSLPRLVNALGIRGVGEVGAGDLARTFSDLDMLSDASYDELQTVEGIGPNIAQA
                     IVDWFDRPANKAVLEKLRAAGVWPRMMSSELEAEGPKPLDGMTFVVTGTLADFTREGV
                     KDFIETHGGKVTDSVSKKTSYLVLGENPGSKYEKARSLGVMVIDEEGLRSLVISN"
     gene            complement(5065..>5744)
                     /locus_tag="AC16CB_00435"
     CDS             complement(5065..>5744)
                     /locus_tag="AC16CB_00435"
                     /inference="COORDINATES: protein motif:HMM:NF014665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HIR3340958.1"
                     /translation="SFPKGDSKRRAAGFSFLFIVTEALVGAGLVLFEWVGQDASSGRA
                     VSIVVHLVNTFFLLASLVLTSWLATDIPGRGEVKNKPPVILLGIGLLGTIVLGASGAL
                     NALGDTLFPASSLLEGIQQDFSATAHFLIRLRVFHPTIAVVVGIYLIILAGFIRIRVK
                     QPATQLIARLLTISIIIQLGAGMVNLLLLAPVWMQIIHLLLADLVWICLVLLTFSSLG
                     LGYNTEL"
BASE COUNT         1517 a         1550 c         1278 g         1399 t
ORIGIN      
        1 gttcaggcgc atgcacgtgt gcctttctgt ttgtaggttg gggggtggtc cgctaactgc
       61 ccctcaacca tttaaccacg tttctaccag ctttggaacg atcacaatcg ccctaatacg
      121 attgccctac cgtgctataa tagggatacc ggggatgaca ggcttcgacg ggagaggttg
      181 gccccggatt gcgagccgat aggcgccgaa atcgttaaat cagcgcaaaa aacaagtgcc
      241 aatcgcactg aatacgctgc tcttcctctc gctgcataag cgatagcctg agcggtcggt
      301 cctctagata gggccgcgtc cacttgcccc gctctttggc cggaggcacg aatgggcgtc
      361 aaaaagccaa agtgccgtac gctaatgtcg ctaaacgtac gaaagagggg ctttcgggtc
      421 ctagcggctg gccggatgtc ccatttgtcg gtctgggtac taccggtaag atcgaaaaac
      481 cgactacgct cgtagatatc cgcgggagga atctttcgga cgggggttcg aatcccccca
      541 tctccaccat ggtaaaagca caccccagat agaattctgg ggtgtgcttt tacgctagga
      601 ttttgaaaca gaaagtataa agccttttaa gtaggccccc tcaggaaaat ttaatgccac
      661 aggatggtca aatccttgat gcaggcgctc aataattttc gcctccacac ccgcgtccaa
      721 tgctgcacca aatatgatct tttgaaataa atcttcactt accccacctg aacaagagaa
      781 ggttgccaat aatccccccg ggcgcaataa ttttaacgcc aatagattga tatccttata
      841 cccacgacta gctcgctcca cttgagcagt tgtatgggca aatttaggag ggtctaatat
      901 aataagatca aattccctgg cctggtcgcg aaattttcga agggtttgga aaacatcccc
      961 ttcaatccat tctacccggt ctgtagacaa acgattaata gccaaatttg ctttcgccag
     1021 tgcaactgcg tctgccgaaa catcaagcgc ggtaactgct ttagcccctc ctgttagtga
     1081 agacagagca aaaccgcccg tataggtgaa acaatccaag acttcccggt ctcgtgccat
     1141 ttgcgcgatt ttaaatcgat taggtctctg gtccaagtaa aatccggtct tatgaccttt
     1201 ggtaatatcc acccaaaatg atagcttacc ctcctgtatc agaacgcgct cgggtggagc
     1261 tgaacccctc agcaacccag ctcgcttcgg taagccctcc agctcacgca cattcacgtc
     1321 ggatctttcg tagatacgtt caacctgcgt tatttccatc aagatgtccg tcaatatctc
     1381 gcgccagagc tccacaccag cggtcaggta ttgaaccacc aaggtgtcgc cataacgatc
     1441 taccaccaat ccaggcaaat tatccgattc ggcgtgaacc aagcgcatgg catctgtttc
     1501 tacttcgtcg gcaattcgca aattatagag ggtctggcga agagagaccg cctcctgcag
     1561 gcgtctttgg aagaattctc gatcgatgga ttcatcgaga ttccaggtcc aaatccggat
     1621 gcgaatattg gattgcgggc tataggctcc taatgcgagg ggttgaccca gaaaatcctt
     1681 taataaaaca gtctcgccga gagttgggtt tccaaccacc cgatgaatag cccctgaata
     1741 cacccaagga tgttgtctga gtacagattt ttcccgcccg gatttgagaa ttacttcgtt
     1801 cagatgatcc ctactttttt gcctgcatcc tcaatgatgt ccatagcatg ctcgatttca
     1861 tccatttcat gagcagctgt tatggtagca cgaagacggg ctaaccctgg aggcaccgca
     1921 ggcgaaacaa ccggcaaaac gaataaatca ttgtgctgcg cctcggctgt tacgccaaac
     1981 gcactttcat cgctcccaca aagtactggc acaatggcgg tttgtgtgta catcgtatca
     2041 aacccgcgct ctttaagacc attaatgaat tgacgggtat tggcattcag ttttgtgacg
     2101 cgccaaggtt catcaaggat cacgtcaaaa gccgcattcg ctgccgcggc ctgcgccggt
     2161 ggcaacgcgg ccgagaagat gtatgctcgg ctagcgtgtc gcaagtattg tattaaatcg
     2221 cgatttccgg ctacatagcc gccaactgat gggattgttt tgctcaaggt gcccattttg
     2281 atatcgattg agcctggcat gtcgaaatgc tcttcaatcc ctctcccatt tttacccaat
     2341 acacctaccg aatgggcttc atcgatcatc aaccaggcat tgtagttgcg acaaagctca
     2401 accatttttg gaaaatcgat aatatctcca tccatactga aaacagagtc ggcgatcact
     2461 aatttggttg cgcctccatc agattcggct tgctgcaagc gctgctccaa cgattccata
     2521 tcattatggc gaaaacgcag gaacttagcg ccggacatca ggcagccatc tacaatactg
     2581 gcatgattta gcttatctga aattacataa gagccacgtc cgatcaaagt cgagacaaca
     2641 gtcaggttgg tcacgtaccc agatgaaaag gtaatcgccg cctccgcccc cttaaaatta
     2701 gcgatttttt cctcgagctc tttgtgcagc gtcaatgaac ctgctagcga gcgaacgcca
     2761 tgggtaccgg taccatattg gtcaatagct gccttcgcgg ctgcattaat tcgtggatgt
     2821 ccaatcaaac ccaagtagct ataggacgca tacatcccca tttcgcggcc attcaccacc
     2881 acgcgcgccc cgccacgcaa ttctttgatg ggttgattgt aaaaatacag cccaagttct
     2941 ttagtaatgt tgacgcgctc attcattgcc tggatgcgtt cagtgatagc atcgttgatc
     3001 cgctgaccca taaatatctc ctcgtcggtt aatttgatat tactaaactg cgtagacctt
     3061 cttcatcaat gaccataacg cccaaagacc ttgctttctc atattttgag cctggatttt
     3121 cacctaaaac caagtagctg gtctttttac tcaccgaatc agtcactttt ccgccatgtg
     3181 tttcgataaa atccttgacg ccttctcttg taaaatcagc aagagtccct gtaaccacaa
     3241 aagtcatacc gtctaaaggc ttaggacctt cagcctccag ctctgaactc atcatacgtg
     3301 gccagacgcc tgctgcacgg agtttctcca aaaccgcctt attcgctgga cggtcaaacc
     3361 aatccacaat ggcttgggcg atatttggtc caatgccctc aaccgtttgc aattcatcgt
     3421 agctggcatc tgagagcatg tccagatcgg aaaaagtgcg cgctagatca cctgcaccca
     3481 cctcccctac ccctcgaatg cctaatgcat tgaccaagcg gggcagagat tgattctttg
     3541 acaactcaat cgactggagc aagttatctg cctttttctc tgcaaaacct tcgagaccga
     3601 gcaaatcttc atgcttcagt gagtacaaat ccgctacgtc tttgataaaa cccgaatcca
     3661 cgagctgctc aacgattcgg atacccaacc cgacaatatc catagctcca cgcgagacaa
     3721 agtgctccaa attgcgaatc aattgggcag ggcaggcaga attcacacaa taccaggcta
     3781 cctctccctc aagatgctca acaggttgct cgcatgcagg acagacctct gggggtataa
     3841 attctctctc ctgtcctgta cgcgctgttg ttagcggacc tattacatag ggaatgacat
     3901 cacccgcacg cttgactgct actcggtcac cgattcgtat atctttgtcg gcgatgtaat
     3961 caaaattgtg caatgtggct tgacggacaa tcacaccccc gatctcaacc ggttccagga
     4021 tagcataggg agttaataca cctgtacgac caacattaac accgataccc attaaagtgg
     4081 tggtcacctc ccgcgcggga aacttgaacg cgatggcacc gcgtgggtcc ttgccaacca
     4141 cacctaaatt ttctcccaaa cccaggtcat taatcttgat aactacacca tctgcctcaa
     4201 aaggaagttg gtctcttcgc tcaactaatc ggttgtatgc ctggatggca tcatctaggc
     4261 tgtcgcaatg ctcctcatca atgacaggaa agcccaaggc acgcaacata cctagtgttt
     4321 cggtgtgact ctcagaaagg ctgccatccc catcaaccac atcataggtt agcagcgtca
     4381 aaggacgagt agcagttaga gttggatcga gctgccgcaa ggctcctgca gcagtattgc
     4441 gcggattctg gtaggttttt tcgccccgct cttctaattg tcgatttagg gcctcaaaat
     4501 cggccggagt gataaaagct tcaccacgca caaccaaata ggatgggggc atgggcccat
     4561 ctacggatat agggattctc aagggcagag cacgaattgt tcgcaggtta gctgtgatat
     4621 cttcgccgat ttcgccatcg ccgcgcgtcg caccttgcac aaaaatccca ttgcggtaat
     4681 gcaagaccac ggttaatcca tcaatctttg gctccacaac gaaatcagcc tgaattaccc
     4741 tctcgtccag cttcaagata cgatcaaacc aggcacgcac gccatccgca tcaaaagcat
     4801 ttccaagact caaaatgggt gctgggtgtc taaccttaat aaatttctct gatgggactg
     4861 agcctgcacg ttgggtcggt gagtcagtcg tgatccaatc tggatgctct tcttcgattt
     4921 gccgaagctc caacatcagt tggtcaaact cgtaatcact gatcaatggg gaatctaata
     4981 catgataccg ataattgtgg taatgaatct cgcggcgaag attctcgatg cgatcgcgca
     5041 gactctggtc aacatcagag atgttcatag ttcggtatta tagcccaaac caaggcttga
     5101 aaaggttaga agaacaaggc agatccagac caaatctgcc agcaacaagt gaatgatctg
     5161 catccaaacc ggcgccagca ataaaagatt taccatacca gcgcctaatt gaataatgat
     5221 gctaatggtc aggagccggg cgataagctg ggttgctggc tgttttaccc gtattcgaat
     5281 aaaaccagcc aggataatga gatagattcc cacaacgacc gctattgtgg gatgaaaaac
     5341 ccgcaaacga attaagaaat gggcagtggc agaaaaatct tgttgaatcc cttctaataa
     5401 cgagctggca gggaacagtg tgtctcctaa ggcgtttaga gcgccgctag cgccgagcac
     5461 gatagtaccc aacaacccga tgcccaacaa tatcacaggt ggcttgttct ttacttctcc
     5521 cctccctggg atatcggttg ccagccagga agttaacacg aggcttgcca agaggaaaaa
     5581 tgtgttcacc aaatggacaa caatcgaaac tgcgcgtccc gatgatgcat cctgtccaac
     5641 ccattcaaac aatactagac cagcaccgac caaggcctct gtcacgatga ataggaagga
     5701 aaaccccgca gcccgccttt tgctatcgcc cttcgggaag ctcc
//
LOCUS       DBNGSX010000016        10765 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_416299, whole genome shotgun sequence.
ACCESSION   DBNGSX010000016 DBNGSX010000000
VERSION     DBNGSX010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10765)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10765)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10765
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_416299"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(313..1836)
                     /locus_tag="AC16CB_00440"
     CDS             complement(313..1836)
                     /locus_tag="AC16CB_00440"
                     /EC_number="4.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016841 - ammonia-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0170035 - L-amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAL/PAL/TAL family ammonia-lyase"
                     /protein_id="HIR3340959.1"
                     /translation="MTIVIDGSDLTVEKMVRIARDGERVELHPEALDRIKACRGMLEE
                     KLKAKEIMYGTNTGIGEFSEVVLTDEQVEQFQRYLIYNHAAGIGEPVSIENVRGAIAS
                     RINVHAHGMSGCRPEITLTLVDMLNKGVTPVVCQKGSVGACGDLAPMAQIALLLMGEG
                     EAYFQGERLPGALAMERAGIPVPGLQARDGLATINGSNLITAISALHLYDMNRWLKQA
                     EIACAMSLEALKANLKPYDVRLHQARGFSGAVRSAKAIMQCIAGSDLVTGKMKTKVQD
                     AYSMRSSPQVIGAAHDAVAFARSQVEIELNGVGDNPIFMPEYNLTLTGANFQGSPVSL
                     PMDMAGAAITMVCVLSERRMNRLTNPALSVGLPAFLTKGAGMFSGLMLSQYTADSLIV
                     EQRILSMPASIQSIPAAADQEDFVSMGMNTALKNNQILDNAYGVLGIEFMAAAQALDF
                     REFTPGHGVQVARQVIRKHVAYLDEDRPLYPDHNAMKALVQSCEILQAVEDEVGSLG"
     gene            2136..2537
                     /locus_tag="AC16CB_00445"
     CDS             2136..2537
                     /locus_tag="AC16CB_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340960.1"
                     /translation="MSLTLQRLNPNLPTTYWLIWLGSFAVPSLSLYLTNQRDISISQA
                     ALMVSLFGIASFTASLAGGELADRLSFHGGDKFHRDLAKDGVCRNSPGGEISRAFPEM
                     RRIHSLQRSPHSIRCAAGECRCHVQSISTIR"
     gene            2553..3206
                     /locus_tag="AC16CB_00450"
     CDS             2553..3206
                     /locus_tag="AC16CB_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013402226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HIR3340961.1"
                     /translation="MEEKMQLLTDLHLAIIDGQRDQVVDLVKRLLEQHADPEIILRNS
                     MIPAMDIVGKRFEEELFFVPEMLIAARAMQAGLDILQPSLAASNIEPIGNVVIGTVQG
                     DIHDIGKNLVAMMLGGAGFRVVDLGVNVPAQKFVDAVQNENGVQIVSLSALLTTTMVN
                     MSAVLEALDKAGLRPDIKVMVGGAPITEAYAQKIGADGYAPDASQAVGLAKRLLGIV"
     gene            3274..4527
                     /locus_tag="AC16CB_00455"
     CDS             3274..4527
                     /locus_tag="AC16CB_00455"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HIR3340962.1"
                     /translation="MPSVQTTAVHASSPDMNRIFHRLNLNLPPTYWLIWLGTLINRLG
                     SFVIPFLTLYLTNQRGISISQAALMVSVFGIGSFIASLTGGELADRLGRRPVMLISFL
                     IAPLNMVLLGLARPVALIATLTLLQGFFTDLYRPAVNAVVADLVEPEGRPRAYGYIYW
                     AINLGAALAPLIAGLMARLDYFLLFLGDAITTFIFGLIVLWGVRETRPAEAQITTRAA
                     VRDRITKLGQEPLLLLFAGLALVFGMIYMQGYVTLPVDMQANGMRPDQYGLVISVNGA
                     LIVLLSIPVSNSAVRWPRFTALAAAALLMGMGYGLTAISSTMLLYAISVAIWTLGEIA
                     GAAVAPSVVADLSPVELRGLYQGVFGAAWGLSFFVGPMIGGWVLEHLGGWALWGGCFA
                     LGCLLALGYLAMTRPAQRRMARTSG"
     gene            4624..5070
                     /locus_tag="AC16CB_00460"
     CDS             4624..5070
                     /locus_tag="AC16CB_00460"
                     /inference="COORDINATES: protein motif:HMM:NF020977.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIR3340963.1"
                     /translation="MSVKSELENALKDAMRAGNDLHKRTLRMALSAIKQTEIDKGITL
                     DDSGVMAILQKEVKTRREAIADAERANRPDLTAASQAEITFLESFLPKELTPEELEDL
                     ARQAIAETNASSIREMGQVMKVLMPRLQGRATGDVASQTVRRLLAG"
     gene            5081..7240
                     /locus_tag="AC16CB_00465"
     CDS             5081..7240
                     /locus_tag="AC16CB_00465"
                     /EC_number="3.1.4.59"
                     /inference="COORDINATES: protein motif:HMM:NF019317.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0106409 - cyclic-di-AMP phosphodiesterase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD family phosphohydrolase"
                     /protein_id="HIR3340964.1"
                     /translation="MKVNGYRIVLLVVAVALSFIALVAPQLIPRSSQSLQAGQVAIQD
                     ILAPRDISFESEILTGQQREAVIRAVAPVYTPADTSISRRQLEKLRNALAFITSLRAD
                     PYASIEQKLTDLAALEDIQLSQETALRILALNDSRWQVIQQETIVVLEQVMRNTIRED
                     RLDDARRSVSSLVSLSLPEDQADIVTRLASALVAANSFYSEALTENARQEALDGISPI
                     IREYKAGETVVQRGQIISETDLEALGQLGLIQTQFNWKELVSAAAIVFLGIGFNLLYL
                     RYNTKIREDARGLAVITVLFLVFLLSARLIISGHVVLPYVFPLAAYGLTVAVLFGAEP
                     ALVSSLSLAILVAYGYPNALDLTLYYALSSFFGVLSLGRGQRILTFFWAGAVIASSGS
                     LVAIAFRLPLPETDWVGITTLVGAALLNGTASASLAVLLQFFLAQLLGMTTALQLLEI
                     SRPDHPLLQFALRNAPGTYQHSLQVANLAEQAAEHIGADALLTRVGALYHDVGKALNP
                     AFFIENQMPGNPNPHDQLDPLPSASIITKHIKDSLELARKYRIPRRLQDFMLEHHGTM
                     IARYQYVNAVKAAGGDESKVDPELFRYPGPRPRSRETAILMLADGCEARVRAERPKDT
                     DEMRALIKSVIQNRMSQGQLDDTDLTLRDLDAIAESFTTTLRGVYHPRIEYPNLEKTT
                     PSATEAVPTLPTSAQTAQKANPSPKSNPPTPAPDSSS"
     gene            7237..7704
                     /gene="ybeY"
                     /locus_tag="AC16CB_00470"
     CDS             7237..7704
                     /gene="ybeY"
                     /locus_tag="AC16CB_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR3340965.1"
                     /translation="MIHLQVSESLAKSIPIAPEILSDLERAGMQTLQQAGIQGSDLTI
                     VITDDAELHELNHRFLGIDTPTDVLSFPTDETDPDSGVRYLGDILISYPRAVAQADAG
                     RHPIQDELRLLVVHGVLHLLGHDHAEPDERDRMWELQGEVLKQLGSSVTLPKE"
     gene            7729..8121
                     /locus_tag="AC16CB_00475"
     CDS             7729..8121
                     /locus_tag="AC16CB_00475"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HIR3340966.1"
                     /translation="MFSFLSSRARAFRYAFRGWSFVIRTQRNAWIHAVISVAVIIISA
                     WLRLSPRDWAGIIIAIAMVWTAEFINTALEAVVDLASHHEHHELARVGKDVGAAAVLI
                     AAMAAILIGFLILGPPLWERLRPLVAGP"
     gene            8165..9016
                     /locus_tag="AC16CB_00480"
     CDS             8165..9016
                     /locus_tag="AC16CB_00480"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HIR3340967.1"
                     /translation="MSSTSFRFGTVGSPISTPSKPGGSVGAVLRSAELDLFALELGWV
                     RAVRVSPETCAAIQATAREQDVLISVHAPYYINLNANDEEWPKSRQRLMDAAHYGNLA
                     GASDIVFHPGSYFERPADEVLPTAISRLQGCIEELRQASNPVTLRPETMGKSAMLGSL
                     EDTLKMSQAIPGVQPCLDFAHLHARTGDGSMNTYDEWSGALDAYGKALGESALQNLHV
                     HLSGIEYGEKGEREHLPLQESDLDLGAILRALHAFGCQGRLLCESPVMEDDAAYMRQV
                     WAEASGE"
     gene            complement(9023..9772)
                     /locus_tag="AC16CB_00485"
     CDS             complement(9023..9772)
                     /locus_tag="AC16CB_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340968.1"
                     /translation="MDSFDYFDRERPIHRIMNFIWNVLTIVMLLGVLCVAGYFLLVFT
                     NPDTPFNPLRPQPLPSPISLPTSTSTPRSILPPTWTPVPTLEPTDTPTPLPTDTPTPT
                     ETPFSLFTPTETATLEGARGMPFDVSPGTPVSTSSLAFHPEVNCDWMGVAGQVFDLSG
                     APISGQQVRIGGFLGNQPVSMLTLTGLTNAYGTAGFYEFKLADKPSATFGSMWLQLLD
                     QAGLAMSDKIFFDTFDSCEKNLIFVNFKQVR"
     gene            complement(9797..10675)
                     /locus_tag="AC16CB_00490"
     CDS             complement(9797..10675)
                     /locus_tag="AC16CB_00490"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3340969.1"
                     /translation="MFLILVAAVVLAGCSRETPAPSASPPPTTSIMASSAPTQSQPSP
                     TPAPLAATVNGEAILLSDYQAELSRYQADVGTELATEDQQRVLNELIDQLLLAQAAAE
                     AGFVVDETILQERYAGLVSQLGSDQALRDWMDANAYTEESFRRTLARSVAAAWMRDQI
                     AAEVPSALEQVHIRQILLYNSEEADRVIAQLQSGEDFADLAALNDPAAGGDLGWVPRG
                     YLLDAKLDEAAFSLQPGEFSAVIQTSAGYHIVQVVEREPLLPLEFDARLALQRRAVQD
                     WLVARRGQSQIQVFTP"
BASE COUNT         2299 a         3199 c         2943 g         2324 t
ORIGIN      
        1 accctccaaa gtcatggcta ccatcgctac cagaggagta gcgacgcgta tgcccaataa
       61 ttctggcaaa aaagccatag ccaccaacgc cgagccaaat cccgccaggc tctgtgtgaa
      121 tacagccaaa aagatgatga aaacgatgag aagctgatcc atatctccca attaacggga
      181 caattctaac cgatcggcta gaattcgcaa tccctttact atattcgctc ccagacaatg
      241 acaggcaccc tcaagcaata aaaatccccc ttcgcctaga agggggatca caaatattga
      301 tcactggagg ctttatccca aactacccac ctcgtcttca accgcctgca aaatctcaca
      361 cgactgcacc aaagctttca tcgcattgtg gtccgggtaa agaggccggt cttcatccag
      421 gtaggcgaca tgcttgcgga tcacctggcg agctacctgc accccatgcc cgggagtgaa
      481 ctcacggaaa tccaaggctt gggcggcagc catgaactca atgcccagca ccccgtaggc
      541 attatccaat atctgattgt tttttaaggc agtgttcatg cccatagaga caaaatcctc
      601 ctggtctgct gctgccggta tggattggat gctggctggc atggagagaa tgcgctgctc
      661 cacgatcaag ctatcggcgg tgtactggct gagcatcagc ccagagaaca tgcccgctcc
      721 cttggtcagg aaagccggca ggccgacgct gagagccggg ttagtcaagc ggttcatgcg
      781 ccgctcggac agcacacaca ccatggtgat ggccgcgcct gccatgtcca ttggcaatga
      841 aaccggcgaa ccctggaaat tcgccccggt taatgtcaga ttgtactccg gcatgaagat
      901 ggggttatcg ccgactccat tcagctcaat ttcaacttga ctgcgggcga aggcgactgc
      961 gtcgtgcgcc gcgccgatca cctggggtga agagcgcatg gagtaggcat cctgtacctt
     1021 ggtcttcatc ttgccggtca ccagatcgct ccctgcaatg cactgcatga tagccttggc
     1081 gctgcgcacg gctcctgaaa agccacgcgc ctggtgcagg cgaacgtcat aaggcttcaa
     1141 gttggctttc agggcttcca ggctcatggc gcaagcgatc tcagcctgct tcagccaacg
     1201 gttcatgtcg tacaggtgca aagcactaat agcagtgatc aagttggagc cattgatggt
     1261 tgccagccca tcccgcgcct gtaagccggg aactgggata cccgcccgct ccatagccag
     1321 ggcgcctgga agccgctcac cctggaaata agcttcgccc tcacccatca gcaacagggc
     1381 tatctgtgcc ataggtgcca ggtcgccaca cgcccccaca gatcccttct ggcacaccac
     1441 cggtgtaacc cctttgttga gcatgtccac cagggtcagg gtgatctcgg gacggcagcc
     1501 cgacatgccg tgggcgtgca cattaatgcg gctggcgata gcaccgcgca cgttttcaat
     1561 ggagactggc tcgccgatac ccgcggcgtg gttgtagatc aggtatcgtt ggaactgctc
     1621 tacctgctcg tcggtgagca ccacctcgga gaactcacca atgccggtgt tcgtgccgta
     1681 catgatctcc ttcgccttga gtttctcctc cagcatgcca cggcaggctt taatacgatc
     1741 cagcgcttca gggtgcaact ccactcgctc gccatctctg gcgatgcgta ccatcttctc
     1801 aactgtcaga tcggaaccgt ctataacgat cgtcatgtgg ccattctcct tgatctgagt
     1861 tctgattact aaaaacggcg aggcagggtt tcttggcatc aaagtcagac ggggaaaacc
     1921 cgatgatctt tgaccgcttc tgcttccgca cttcctgaga ggttcgggcg tttgtcttta
     1981 taattatatc cccgaaggca gacttacaac ctgtgtgagg tgcattcagt tacaatttaa
     2041 tgaagacgtg aagctggagg agttcggaga agctcgatat caatttatta atttcccaac
     2101 acatttcctg taatatactt agtgctgtgt cttccatgag cctaacactt cagcgtctca
     2161 accccaacct gccgacaaca tactggctca tctggctggg cagcttcgcc gtcccctccc
     2221 tgtcacttta cctgaccaac cagcgggata tctcgatctc acaggcggcg ctgatggtat
     2281 cgctgtttgg catcgcgtca tttacagcat ccttggcagg cggtgagttg gccgaccgtc
     2341 tgagtttcca cggtggggat aaatttcatc gagaccttgc caaagatggc gtctgccgaa
     2401 atagcccagg aggtgaaata tcgcgtgcgt ttcctgaaat gcggcggata cattctctcc
     2461 agcgctcacc acattcaatc cgatgcgccg ctggagaatg tcgttgccat gtacaatcca
     2521 tatctacgat acgctgaggg ttgataaaaa ttatggaaga gaaaatgcaa ttgcttaccg
     2581 atctgcacct ggcaataatc gacgggcaac gcgatcaggt ggtggatctg gtgaaaaggc
     2641 ttttggaaca gcacgccgac ccggaaatca tattgaggaa ttcgatgata ccggcaatgg
     2701 acatcgtggg caagcgtttt gaagaggagc ttttctttgt acctgaaatg ctgatcgccg
     2761 cacgagcaat gcaagccggc ctggacatcc tccagccgtc gttggctgcg tcgaatatcg
     2821 aaccgattgg aaatgtcgta ataggaaccg tgcagggcga tatccacgat atcggcaaga
     2881 atctagtcgc catgatgctc gggggtgccg gttttagggt ggtggacctt ggcgtaaatg
     2941 tacccgcgca gaagtttgtg gatgcagtgc aaaacgagaa cggggtgcaa atcgttagtt
     3001 tgtcggcatt gcttactacg acaatggtca acatgtcggc ggtgttagaa gcgctggata
     3061 aagctggcct gcgcccggat attaaggtga tggtaggcgg tgccccgatt acggaagcct
     3121 acgcccaaaa gatcggtgcg gacggctatg ccccagatgc cagccaggct gtcgggttgg
     3181 ccaagcgcct actaggcatc gtttaagctc cgctcttatc ctgagagtcc ttgacccaag
     3241 aaaagcgctt ttgtcttgaa agatgaaggc cggttgcctt ctgtccaaac aaccgctgtt
     3301 cacgcctcaa gcccggacat gaaccgaata tttcatcgcc tcaacctcaa cctgccgcct
     3361 acctactggc tcatctggct gggtacgctc atcaaccggc tgggcagctt cgtcatcccc
     3421 ttcctgacac tttacctgac caaccagcgg ggtatctcga tcagccaggc ggcgctgatg
     3481 gtctctgtgt ttggtatcgg atcatttatc gcttcgctga caggcggcga gctggccgac
     3541 cgcctgggcc gccggccggt catgctgatc agcttcttga tcgctccgct caacatggtt
     3601 ctccttggac tggcgcgccc ggttgcgctg atcgccactc tcaccttgct ccagggcttc
     3661 ttcactgatc tgtaccgccc ggcggtcaac gccgtggtgg ctgacctggt agagccagaa
     3721 gggaggccgc gcgcctacgg ctacatctat tgggcgatca acctgggcgc cgcacttgca
     3781 ccactcatag caggcctgat ggcgcgcctg gattactttt tgctgtttct tggcgatgca
     3841 atcaccacct tcatttttgg cctgatcgtg ctgtggggtg tgcgtgagac gcggcctgcc
     3901 gaggcgcaaa ttaccacccg agccgccgtg cgagatcgca tcaccaagct cgggcaagag
     3961 cctcttttgt tgctcttcgc cgggttagcc ctggtctttg gaatgatcta catgcaaggg
     4021 tatgtcacgt tgccggtgga catgcaagcc aatgggatgc gcccagatca atacggatta
     4081 gtgatctctg tcaatggcgc gctgatcgtg ctgcttagca taccggtgag caactcggcg
     4141 gtgcgctggc cgcgctttac tgcgctggct gcggcggctt tgctcatggg catgggatac
     4201 ggcctgacgg ccatctcgtc aaccatgttg ctgtacgcca tctcagttgc aatctggacc
     4261 ctgggcgaga ttgctggcgc tgcggtagcc ccctcggtgg tggcagacct atccccggta
     4321 gagctgcgcg gcttgtacca gggcgtcttc ggcgccgcct gggggctttc gttctttgtc
     4381 ggccctatga tcggcggctg ggtgttggag catctcggag gctgggccct ttggggggga
     4441 tgcttcgcat tggggtgcct gctggctctg ggatacctgg cgatgacacg ccccgcccaa
     4501 cggcgcatgg cgcgtacttc aggctaaaat gtcagcgtcg tcatggggcg tgtataagcg
     4561 gagcaccttt ctcacctcca ctgcccatgc tgttgaatat gaaatattag gtataatgat
     4621 ctcatgagcg tcaaatccga actcgaaaat gccctcaaag acgccatgcg cgctggcaat
     4681 gacctgcaca agcgcaccct gcgcatggcg ctctctgcta ttaagcaaac cgagatcgat
     4741 aaaggcatta ccctggacga ctcgggggtg atggcgatcc tgcagaaaga ggtcaaaacg
     4801 cggcgcgaag ccattgcaga cgccgagcgc gccaaccgtc ccgacctgac cgccgcatct
     4861 caggcagaga tcaccttctt agaaagcttt ctccccaaag aactcactcc agaggaactg
     4921 gaagatctgg cacggcaggc tatcgccgaa acgaacgcta gctcaatccg tgagatgggt
     4981 caggtgatga aagtgctcat gccacgcctg cagggtcggg caactggaga cgtagccagc
     5041 caaacggtgc gcaggctgct agcaggttaa gggtttcatc ttgaaagtaa atggctacag
     5101 gattgtattg ctggtggttg ccgttgcgct ctcgttcatc gcgctggtag ccccccaact
     5161 cattccccgc tcttcgcaat ccctgcaagc tggacaggtt gctatccagg atatcctggc
     5221 cccgcgcgat atctctttcg agagcgaaat ccttaccggg cagcagcgcg aggccgttat
     5281 tcgggctgtt gccccagtct ataccccagc cgacaccagc atctcccgcc ggcagctgga
     5341 gaaacttcgc aatgccttgg ccttcatcac cagtctacgc gcggacccct acgcctccat
     5401 agaacaaaaa ttaaccgatc tagccgcgct ggaagacatc cagctcagcc aggagactgc
     5461 tctgcgcatc ctggccctca acgattcgcg ctggcaggtc atccagcaag agaccatcgt
     5521 agtcttggaa caagtgatgc gtaacacgat ccgggaggat cgcctggacg atgcccggcg
     5581 cagcgtctct tccctggtca gtttatctct tcctgaagac caggcggaca ttgtcacccg
     5641 actcgcctca gctttggtgg cagccaacag cttctacagt gaagccctta ccgagaacgc
     5701 ccgccaagaa gccctggatg ggatcagccc gatcatacgg gaatacaaag ccggcgagac
     5761 ggtggtgcag cgcggccaga tcatctccga gaccgatctg gaagccctgg gacagctggg
     5821 gctaatccag acgcaattca actggaaaga gttggtcagc gcggcggcca tcgttttcct
     5881 ggggattggt tttaacctgc tctacctgcg ctacaatacc aaaatacggg aagatgcccg
     5941 tggcctggca gtcatcaccg tactgttcct ggtcttcctg ctgagcgcgc gcctgatcat
     6001 ctcggggcac gttgtcctgc cttacgtctt tccattggca gcttacggcc tgacggtggc
     6061 tgtgctattc ggcgcagagc ctgccctggt ctcgtcgcta tccctggcga tactggttgc
     6121 atatggttat ccaaacgccc tggatctgac cctgtactat gctctgagca gtttctttgg
     6181 ggtgctcagc ctggggcgag gccagcgaat cctgaccttc ttctgggcag gcgcggtaat
     6241 cgccagtagc ggcagcctgg tagccattgc cttccggtta cccctgcctg aaaccgattg
     6301 ggtcggcatc accaccttgg tcggggcagc cctgctgaat ggcaccgcct cggccagcct
     6361 ggctgtgctg ctgcaattct tcctggccca attattgggc atgaccactg ctttgcaact
     6421 cctggagatt tcacgtccag accacccctt gctgcaattt gcgctgcgca atgcgcctgg
     6481 tacctaccag cacagcctgc aggtggcaaa cctggccgaa caggcagccg aacacatcgg
     6541 cgctgacgct ctgctaaccc gggtgggcgc actgtaccac gatgtgggca aagccctcaa
     6601 cccggccttc ttcatcgaaa accagatgcc cggcaatccc aaccctcatg atcaactaga
     6661 tccgttaccc agtgcatcga tcatcaccaa acacatcaaa gatagcctgg agctggcgcg
     6721 caagtatcgc atccctcgcc gcctccagga tttcatgctg gagcatcatg gcaccatgat
     6781 cgcccgttac cagtacgtga acgccgtgaa ggccgctggg ggtgacgaaa gcaaggttga
     6841 tccggagctc ttccgctatc caggtccacg ccccagatcg cgtgagaccg ccatcctgat
     6901 gcttgccgac gggtgtgaag cccgggtgcg cgccgagcgc cccaaagaca ccgacgagat
     6961 gcgcgccctg atcaagagtg tgatccaaaa tcgcatgtcc cagggacagc tagatgatac
     7021 cgatctgacc ctgcgtgacc tggatgcgat tgccgagtca ttcaccacca cgctacgggg
     7081 tgtttatcac ccgcgcatcg aataccccaa cttggaaaaa accacccctt ccgcaaccga
     7141 ggcggttccc actttgccca ccagcgccca aactgcgcaa aaagcaaatc cttctccaaa
     7201 atctaatcca ccaactcctg cccccgattc atcatcatga ttcacctgca ggtctcggaa
     7261 tctctcgcca aatccatccc gatagcacct gaaatcctgt ctgatctgga acgcgctggc
     7321 atgcagaccc tgcaacaggc gggcatccaa ggctcagatt tgaccatcgt catcacagac
     7381 gacgctgaac ttcatgagct caaccaccgc ttccttggaa tcgacacccc cacggatgtg
     7441 ctctccttcc ccacagacga aactgacccc gattctggcg tgcgttatct aggcgacatc
     7501 ctcatatcct acccgcgggc tgtcgcccag gcagatgcgg ggcgtcatcc aatccaggat
     7561 gaattgcgcc tcctggtcgt gcatggggtg ctacacttgc tgggccatga ccatgccgag
     7621 ccggatgagc gtgaccgcat gtgggagcta cagggggagg tcttgaagca actgggcagc
     7681 agcgtcacgc tgcccaagga atgatcatcg atccagcaaa acacccttat gttctccttc
     7741 ctgtcctctc gggcccgcgc ctttcgctac gcttttcgcg gctggtcgtt tgtcatccgc
     7801 acccagcgca acgcctggat tcacgccgtg atcagcgtgg cggtcatcat catctcggcc
     7861 tggcttcgct tatcgccgcg tgattgggct ggaattatca tcgccatcgc catggtgtgg
     7921 accgctgaat tcatcaacac ggcgctggaa gctgtggtag atctggccag ccatcacgag
     7981 caccacgaac tggctcgcgt gggcaaagat gtcggcgccg cagccgtgct gattgctgcc
     8041 atggctgcca tactgatcgg ttttctcatc ttgggtcccc ccttgtggga gcggctgaga
     8101 cccctcgtgg ctggaccctg atcgccggcc cttccctatt gaatcatcga acctggagct
     8161 atctatgtct tctacatcat ttcgctttgg cactgtcggt tcgcccatct ccaccccctc
     8221 caagcccggt ggcagtgtgg gtgctgtgtt gcgttccgcc gagctagacc tatttgccct
     8281 ggaactaggc tgggtgcgcg ccgtgcgcgt ctcccctgag acctgcgcgg ctatccaggc
     8341 aaccgcccgg gagcaggacg tgctcatcag cgtgcacgcc ccctactaca tcaatctgaa
     8401 tgccaatgat gaagagtggc ccaagtctcg ccagcggttg atggacgctg cccactacgg
     8461 aaacttggcg ggtgccagcg acatcgtctt tcatccagga tcatatttcg agcgaccggc
     8521 tgacgaggtg ctgccgaccg ccatatcccg cctgcagggc tgcatcgaag aattgcgcca
     8581 ggctagcaac ccggttacct tgcgccctga gacaatgggg aaatcggcaa tgcttggctc
     8641 gctcgaggac actttgaaga tgagccaggc catacccggc gtacagccct gcctggattt
     8701 cgcccacctg cacgcccgta ctggggacgg ttccatgaac acctacgatg agtggtcagg
     8761 cgcattggat gcatacggga aggcattggg tgaatctgcc ctccagaatc tgcacgtcca
     8821 cctatccggt atcgaatatg gggaaaaagg cgaaagggag cacctgccct tgcaagaatc
     8881 cgatctggac ctgggcgcca tcttgcgcgc cctgcacgca ttcggctgcc aggggcgctt
     8941 gctatgcgag agccctgtta tggaagacga tgccgcctat atgcgacaag tatgggcaga
     9001 agcaagcgga gaataaagcc ttctaccgca cctgtttgaa attaacgaag atcaggttct
     9061 tttcgcagct atcgaaagta tcaaagaaga tcttatccga catcgccaga ccagcctggt
     9121 cgagtaactg taaccacatg ctgccaaagg tggcgctggg tttatcggcc agtttgaatt
     9181 cgtagaatcc ggcggtgccg taagcgttgg ttaaccccgt cagagtgagc atgctcaccg
     9241 gctggttgcc caaaaagccg ccaatgcgca cctgctgacc cgagatcggc gcgccggaga
     9301 gatcaaacac ctgtccggct acacccatcc aatcgcaatt gacctccggg tgaaaggcga
     9361 gactggaggt gctgaccggc gtgcccggac tcacgtcgaa aggcatccct cgcgccccct
     9421 cgagcgtggc agtctcggta ggggtaaaca agctgaaggg tgtctcagtg ggagtaggcg
     9481 tgtccgtggg cagcggcgtg ggggtatcgg tgggctctag ggtaggcacg ggcgtccagg
     9541 tagggggcaa aatgctgcgc ggtgtggacg tggaggtagg caaggatatc ggcgaaggta
     9601 gcggctgtgg ccttaagggg ttaaaaggcg tatctgggtt ggtaaaaacc agcaagaagt
     9661 agccggccac gcacaacacc cccagcaaca tgacgatggt cagaacgttc cagatgaagt
     9721 tcatgatcct gtggataggg cgctctcgat cgaagtaatc aaagctgtcc atgctctcct
     9781 ctttccactg atggatttac ggtgtaaaaa cctgaatctg gctttggccg cgacgcgcca
     9841 ccagccaatc ctgcacagcc ctgcgttgca gcgccaaccg ggcatcgaat tccagaggaa
     9901 gtagtggctc gcgctctaca acctgcacta tatgatagcc tgcactggtc tggatcacag
     9961 cactgaactc accaggctgc aagctgaatg ccgcttcgtc taattttgca tccagcaggt
    10021 atccccgagg gacccaaccc agatcgccgc ctgcggccgg gtcgttaagt gccgctaaat
    10081 cggcgaaatc ttcgccagat tgcaactgcg ctatcacgcg gtcggcctcc tcggaattat
    10141 atagcaaaat ctggcgaata tgaacttgct ctagcgcgct cggcacttcg gcggcgatct
    10201 ggtcgcgcat ccaggctgca gccacagagc gggccagcgt ccggcggaag ctctcctcag
    10261 tgtaggcgtt ggcatccatc cagtctctca gtgcctggtc gctaccaagc tgagaaacca
    10321 atcccgcgta gcgctcttgc aagatcgttt cgtctacaac aaaaccggcc tcggccgcag
    10381 cctgcgccag caagagctgg tcgatcaatt cattcaacac gcgctgctgg tcttcagttg
    10441 ctagttccgt gccaacatcc gcctgatagc ggctcagctc ggcctggtaa tcgctcaaca
    10501 ggattgcctc cccattgacg gtcgcagcca aaggagctgg agttggtgaa ggctgcgatt
    10561 gggtgggcgc tgacgaggcc attatagacg ttgttggagg cggggaggcg ctaggcgctg
    10621 gcgtctcgcg cgaacagcca gccaagacaa cagccgctac caagattaga aacacgcagt
    10681 gtttcctcat tgggaagatt atacctattt atgcgaagca tcacaatcaa caattagtca
    10741 tgtttccccc acatttgcac ctgga
//
LOCUS       DBNGSX010000017         1714 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_901284, whole genome shotgun sequence.
ACCESSION   DBNGSX010000017 DBNGSX010000000
VERSION     DBNGSX010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_901284"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..595
                     /locus_tag="AC16CB_00495"
     CDS             98..595
                     /locus_tag="AC16CB_00495"
                     /inference="COORDINATES: protein motif:HMM:NF016089.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TQO small subunit DoxD"
                     /protein_id="HIR3340970.1"
                     /translation="MLKSIFKKIDNIDSLTWIALLRMMVGLMFLSTWYSNQLKGFYTP
                     DGLLEFFTNVFPQSENPLGWYAAFIQGIILPIRHLFAPFQLVAEFLLGLALLVGGLTP
                     LFSLAGIVFLTNTFLATFGHDWPWAYMLPIGILGVVFLARAGRSLGVDAILLRRFGER
                     GRFLW"
     gene            710..1471
                     /locus_tag="AC16CB_00500"
     CDS             710..1471
                     /locus_tag="AC16CB_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR3340971.1"
                     /translation="MLSVERQQAIVDLVDKNDSIQVADLVKLFDVSEMTIRRDLDILE
                     RKGLLRRVHGGAVSSHGRSYEPPFLLRSTDHSEEKQRIGKAAADLIKKGDSVMLDVGT
                     TTLEIARHLYAHQNLTIITPCFQIAALLAEHPGIRLILTGGILRRSELSMVGHLAERT
                     IQEFYVDKLFVGAGGVDIVTGFTEFNLEDTLVKQAMLRQAKDITAVVDSSKFGQVALT
                     AIAPLKAVNRIITDNGLEPKMADRIREMGIEIVLV"
BASE COUNT          428 a          399 c          447 g          440 t
ORIGIN      
        1 tattgatttg ggcagcctgg cctttgatcc ggcatggtaa gatgtttaag cgcgcagtaa
       61 aacggatcag ctagagggaa tattcccagg agaagaaatg ctaaaaagta tttttaagaa
      121 aatagacaac atcgattcgc taacttggat tgcattattg cgcatgatgg tgggtctcat
      181 gttcctgagc acttggtata gcaaccagct caaaggtttt tacacgccgg atgggctgtt
      241 ggagttcttc actaacgtct tcccgcaatc ggaaaatccg ctaggctggt atgctgcctt
      301 tatccaaggg atcattctgc caatccgtca cttgttcgcc ccttttcagt tggtcgcaga
      361 gttcctctta ggattggcgc tgctggtggg agggctgacg cccctattca gcctggcagg
      421 gattgtcttc ctgaccaata ccttcctggc tacctttggt cacgactggc cctgggcgta
      481 tatgctgccg attggcattc ttggggtggt atttctcgcg cgtgcagggc gttccctggg
      541 tgtggatgct atcttgctga gacgatttgg agagcgcggg cggtttcttt ggtaatgcaa
      601 gacagtttag gaggaaaacg gcttaaactt gttgacaaac gaacaaaaat atgtatcatt
      661 atgaacatat tcgaacgtag gaattgcctc tctcgaaagg cacattctca tgctttctgt
      721 cgaaagacaa caggctatcg tcgatctagt cgataaaaac gactccatcc aggttgccga
      781 tctggtcaaa ttgtttgacg tctctgaaat gaccatccgc cgcgatctgg atattttgga
      841 acgaaaaggt ctattgcgcc gtgtacatgg gggggcggtt agctctcatg ggcgcagtta
      901 cgaacccccc ttcttgttgc gtagcaccga ccatagcgaa gaaaagcaac gcattggcaa
      961 ggcggctgcc gatttgatta aaaaaggcga ctcggtcatg ctggatgtgg gcactaccac
     1021 tttggagatt gcccgtcatc tgtatgctca ccagaatctg accatcatca cgccctgttt
     1081 tcaaatcgca gcgttattgg cagaacatcc cggcatccgc ctcatcttga ccggcggcat
     1141 cttgcgccgc agcgagctat ccatggtggg ccatctggcg gagcgaacga tccaggagtt
     1201 ctacgttgat aagctgtttg tcggggctgg cggggttgac atagtcactg gtttcactga
     1261 attcaatctt gaagatacac tggttaagca ggctatgctg cgccaggcca aagatatcac
     1321 cgctgtggtc gattcgagca aattcggaca agttgcctta actgccatcg cgcccctgaa
     1381 ggctgtaaat cgcatcatta cggacaacgg acttgagccg aaaatggctg atcgaatccg
     1441 tgaaatggga attgaaatag tcttggtata acggcaaatt attggttgaa tcgattccta
     1501 aatgaggaga acacaagacc tggttgctga aaggaggcga atagcaaaaa ttcctggtaa
     1561 aaataatctg tagcatcaac caatcgaaat caatcttgca aggagagaaa aatgaaaaag
     1621 agcctgctga tcttatccat tctgttggtg gttgccctgg tggcatcctg cgcacctcaa
     1681 gcggctccga cagaagaagc tgcacccccc gaag
//
LOCUS       DBNGSX010000018         3099 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1109141, whole genome shotgun sequence.
ACCESSION   DBNGSX010000018 DBNGSX010000000
VERSION     DBNGSX010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1109141"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="AC16CB_00505"
     CDS             <1..597
                     /locus_tag="AC16CB_00505"
                     /inference="COORDINATES: protein motif:HMM:NF028425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HIR3340972.1"
                     /translation="KSVSVKIKMGAGDLKIIGDAEKLLEADFAYNVAKLKPEVDFTDG
                     MLVVQHPDVRGYRTLQDIKDFRNEWDLRLNNDVPMNLSLEMGAGTSALQLSGLSLTGL
                     DVTLGAGKSTVDLTGNWARDLDVTFDTGAADITVRLPRDVGVRVEVDSGLTAISAPDL
                     KQDGNVYTNAAYGVSDMTLQINLKAGIGQINLQVEEGP"
     gene            complement(1406..2785)
                     /locus_tag="AC16CB_00510"
     CDS             complement(1406..2785)
                     /locus_tag="AC16CB_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340973.1"
                     /translation="MRGRIIFRATLSAVLFMLVAGTDLDKVDSSNSSANPGAAAPWFI
                     DEVDTDGDMGLHASVAYDPSRGVTYVSYYDATNQELRLARSNGFGPDPCGPNGKWGCH
                     TVDRGPDVGKYSSVAVNPDTGGIGIAYYDATNGNLKYLIFENPNLLVHNIYTIDKGIE
                     GISSTGLHTSLAYSDEGRPFIAYYFENPSGVDALMFAYYSVTNGNCGYGYAEDTWRCH
                     TIISGEGVGQHPSLVVRNNPSTNDWDFYISFYNGGTGELWYSKTVETEGNCPTDLTDM
                     ICYGVAGGNDVGRYSSLYVDSAGDFHIAYYDATEKRLMYAVELASGTGNCGVLGSAQC
                     DEIDSMQANYHPLGISIAEDPAGYPAIAYQAANGSLKLARPINALGIPAGGGDCGPED
                     LFYLWFCETIDPFGTWINYRNGDFVSLAFSPSGLAHIAYNGFILPEGGNLYVAYQRFN
                     LHLPLVMKD"
BASE COUNT          808 a          772 c          742 g          777 t
ORIGIN      
        1 aagtccgtca gcgtcaaaat caaaatgggc gctggcgacc tgaaaataat tggcgatgcg
       61 gaaaaattgc tggaggctga cttcgcctac aacgtggcta aactcaagcc agaggtggat
      121 ttcacagatg gcatgctcgt tgttcaacat cccgatgtcc gcggctaccg aaccctgcaa
      181 gacatcaagg acttccgcaa cgaatgggac ctgcgcctca ataacgatgt accgatgaac
      241 ctgagcctgg agatgggcgc ggggaccagc gctcttcaac tatccggcct gtcattgacc
      301 gggctcgatg tcacgctggg cgcgggcaaa agcacagttg acctgaccgg caactgggcg
      361 cgtgacctgg acgtcacttt cgataccggc gcggctgaca tcaccgtgcg gctgcctagg
      421 gacgttggag tccgcgttga ggttgattct ggcctaaccg cgattagtgc cccggacctg
      481 aagcaggatg gaaatgttta cacaaacgct gcctatggtg tatcagacat gacattgcag
      541 atcaacctga aggccggcat cggccagatc aacctgcaag tggaagaggg gccgtgatga
      601 atggttactc cattcaaaaa taataggaga aatctcatga ccactcaaga aatcacatcc
      661 aggaaagatc aatgtatggg atctaataat ttgcattctc gattagggcg acggcgctgt
      721 agcaaagaaa tcaatattac atttgccatc ctccaggacc aatcctaaga ataaatcttg
      781 tagttatgtc ccctgcgtga attgaataag atatttcagt ctctcccgat tctcaaagtg
      841 aattaacgca aagaactgct cgacaggtat gtcgaacagt tctattcttt ttagtgcccc
      901 caagtggagc tgactcgaac tacgtgatga tgattctaat ctgtgggagt gataataggt
      961 ttgaattgga attttaaact tgagacctca tttgatgtaa tctgaccatt ctcaaccaag
     1021 taaaagtttg taccgctcaa ttgaattgtg caactgatcg ggtggctaac atacccttcg
     1081 gcttctgcag taacgacata gatcactcct tgattttctg gtagtactac tctcatttcc
     1141 cccatgccgg gatgagatcc tgttagtggt gtatctcccg atagtgtgtg aaaatatatt
     1201 ctgccaagag cactttcgtc taatctggat aatgacacaa ttatgtgcgg ttcttgcggt
     1261 tctggtctta gtggttccgg tggtcattgt gattagtaat aatacaaaca gcaccatagc
     1321 aactcgcagc cagttactgc tgtccgaaaa gcgaaacttc aagtcaccat ttttcctaat
     1381 tcgatgtcat aagctatcta agacattaat ccttcattac caatggcagg tggagattaa
     1441 accgctggta agccacatag agattgcctc cttcaggaag aataaaccca ttgtatgcga
     1501 tatgagctaa tccagacggg ctgaatgcga gggaaacaaa atccccgttg cggtaattga
     1561 tccatgttcc aaaggggtcg attgtctcgc aaaaccacag gtagaataga tcctcaggtc
     1621 cacagtcacc accaccggcg ggtataccca gggcattgat cggacgggca agcttgagcg
     1681 agccgttcgc tgcctggtat gcaatcgccg ggtagccggc tggatcctct gcaatcgaaa
     1741 tacccagcgg atgataattg gcctgcattg aatcgatctc gtcgcactgg gctgaaccca
     1801 gcaccccaca gtttcccgtt ccgcttgcca gctcgacggc atacatcagc ctcttttcgg
     1861 ttgcatcata gtaggcgatg tggaaatcac cggcgctatc cacgtacagg gatgaatatc
     1921 gaccgacgtc gttgccaccc gccaccccat aacagatcat gtctgtcagg tcggtgggac
     1981 agttcccttc ggtttccaca gtttttgaat accagagctc accggttccc ccgttgtaga
     2041 aggagatgta aaaatcccaa tcattggtcg acgggttatt tctaacaacc agggaagggt
     2101 gctgccctac cccttctccg ctgattattg tatggcacct ccatgtatcc tcggcataac
     2161 catagccgca attgccattc gtgaccgagt aataggcaaa cattaatgcg tcaacaccag
     2221 acggattctc aaagtagtag gcaatgaacg gcctgccctc atcactgtac gccagcgagg
     2281 tatgtaagcc tgtgcttgag atgccttcaa tccccttgtc gattgtgtag atattatgga
     2341 ctaaaaggtt aggattctcg aaaatgaggt atttgaggtt accgttggtg gcatcatagt
     2401 aagcgattcc tatccctccg gtatccggat tcaccgcaac tgaactgtac tttcctacat
     2461 ctggacctct gtccacggta tggcagcccc acttaccgtt gggtccgcag ggatcaggtc
     2521 caaaaccatt cgatctagcc aggcgcagtt cctggtttgt ggcgtcataa taactgacgt
     2581 aggtgacccc acgtgacggg tcatatgcta ccgaagcgtg cagacccatg tcaccatcgg
     2641 tgtcaacttc atcaatgaac cacggggcag ccgcgcccgg gtttgcactc gaattggatg
     2701 agtctacctt atccagatcc gtcccagcca ccagcatgaa caggaccgct gagagtgttg
     2761 ctctgaagat gatccgacca cgcatcactt cctcctttcc ttttttggcc tcacgcctct
     2821 caaaaattta tcaggggact gttgaaactc aaggcatcat tgtaaatttg atgtggtcgc
     2881 ccgacagagg gaaaatagta agctgttcag ttggtcatct gcgattcgtt aaatatcttc
     2941 tactgaaatt agaataaatt caccttcata accgagggtc aaactgtcat cgcggtggga
     3001 atgaccctca ttagaacacg atgatcaaaa tgcttttaca cccttaaaaa taatatatca
     3061 gaagttacgc aattggggag gtgatggggg agtttgggc
//
LOCUS       DBNGSX010000019         6011 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1317092, whole genome shotgun sequence.
ACCESSION   DBNGSX010000019 DBNGSX010000000
VERSION     DBNGSX010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6011)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1317092"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..814
                     /locus_tag="AC16CB_00515"
     CDS             131..814
                     /locus_tag="AC16CB_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR3340974.1"
                     /translation="MTKFTWYGHATMGLETGGYKLLIDPYFSENPAATTTVDQVQADY
                     ILVSHGHFDHVTDLLEVARRTEATIITNFEISNWLGQQGLNSHAQHIGGGFKHPFGYL
                     KLTLALHGSGLPDGFYGGNPAGFLLTTLDGKKVYLACDTGLFGDMRLIGEEGVDLAVL
                     PIGDNFTMGPDDALRAVKLIQPQHVIPVHYNTWPLIAQDPQAWASRVKAETSAQVHIL
                     NPGESFTLP"
     gene            complement(960..1904)
                     /locus_tag="AC16CB_00520"
     CDS             complement(960..1904)
                     /locus_tag="AC16CB_00520"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity
                     producing 5'-phosphomonoesters, hydrolytic mechanism
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HIR3340975.1"
                     /translation="MFEIIFLGTSASAPSVHRGLSAQVVMHDEYRFLIDCGEGTQRQI
                     LQSGIGFKRLSRILITHGHLDHILGLAGLLSTFSRWEAIDELEIYAGGWALQRIHDLL
                     YGVVLRGARPPMDIRLREIEPGEFFRDDDFSVSAIPVFHRGPDCFGFLFEEKPRRPFL
                     PEKAEALEIPPGPWRRDLVDGQVVTLPDGRVIHPDQVLGPERAGTRLVHIGDAGRTDN
                     LLQVCRDADALVIEATYLDEESEMAGEFAHLTARQAAELAQQANVQNLILTHISRRYR
                     ERDVLAEAQAIFPHTRVARDFDHFQIRRGEFIKMNEKP"
     gene            2006..4423
                     /locus_tag="AC16CB_00525"
     CDS             2006..4423
                     /locus_tag="AC16CB_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013727.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR3340976.1"
                     /translation="MARQTRKDGRKKPDKSSRPAVKSQKSKPNPGRGASNKKPGRTSK
                     PAARGSSSRQSRQAPGKPASRSKSTPSPRQSFTSGLSLDRKLDIAGVVLSLLGLLTLL
                     SLISSSRSAITSGWIGMLSKGLGWGIYLLPLTLLVVGLWLVLRNFEQIPLPSPERLLG
                     LSLLYLNLLGILHLFKLPETREASLALADAGQGGGYLGALTLEILRTILGMGGATVAL
                     LAWLLIAMALSLDVSVVELFHWLPPLLSRLQDRWNDFRLQRLSKGVSQPELDGFTPLQ
                     RGVDLDYELDPQIANRLAALPSLPPTLGVTPKDWQLPVVEEILEAGGEVSYDTELDKQ
                     RGRVIEETLASFGAPAKVVEISRGPTITQFGVEPDFIESRGGRMRVRVGKIAALADDL
                     ALALSASTIRIQAPVPGKGYVGIEVPNDEISLVTLRSVIENEAFRRVKSPLGFALGQD
                     VAGNAVAADLAGMPHLLVAGATGSGKSVCVNSLITCLLLHNTPEDVRLIMVDPKRVEL
                     TGYNGIPHLLAPVVVELERVVGALQWVLREMDQRYHKLAQAGCRNLKEYNAKLLARGE
                     KKLPFLVVFIDELADLMMLAPDETERTITRLAQLARATGIHLVIATQRPSVDVVTGLI
                     KANFPARIAFAVASGVDSRVILDQPGAERLLGRGDMLFQAPDAAAPVRLQGVFVSDSE
                     IQRLVQYWQSYAGAVALAPTAVGGVVDAVPSGLPLKQIPLWDDFTPEEEKDPLLDEAI
                     DLTRRQGRASVSMLQRRLRIGYTRAARLIESMEAKGIVGPPESGTGAREVLDYGPAAP
                     PIDEQRE"
     gene            4483..4950
                     /locus_tag="AC16CB_00530"
     CDS             4483..4950
                     /locus_tag="AC16CB_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015956747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HIR3340977.1"
                     /translation="MGSQSQRVATPYDELTYRIIGCAMAIHRKLGPGYRENTYQRDLE
                     AHFTDTGLAFEGQKLLEVYDSSKGEQLIGYYIPDFLVENTVVVEIKALRGLDNSHLAQ
                     VIGYLAVTGCPIGLLINFGTRSLQYRRIFPPKDITEHRVNQQWLFVPDWLKNN"
     gene            complement(5112..>6011)
                     /locus_tag="AC16CB_00535"
     CDS             complement(5112..>6011)
                     /locus_tag="AC16CB_00535"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR3340978.1"
                     /translation="MRAQVSRVAELRGSLIDEIFNAMGLPQGWLRRALWPLAWAPAHR
                     FARLVEGFERYNAEYGFSEAARRLLECFIHHVVVSGAEHIPPEGPLLIASNHPGAVDG
                     LAIAASLPRQDLKIVVSGVPFFHNLSFIHEHLIYTAENAHGRMLVLRNAIRQLRQGGA
                     VLIFPSGCVDPDPSLFPDAWRSLQAWSSSLALMLEKAPSTRLLVTAVSGVLAPECWRH
                     PLVRLRQKMWEKQKLAEFIQVIQQLVLGRRYPIVPRVSFAEPLTLSELQLGEKDGDTM
                     QAVRAHARRVMEAHLSLQGEVYR"
BASE COUNT         1303 a         1810 c         1669 g         1229 t
ORIGIN      
        1 tatgtgatgt ttggttgacg ccggatgccc ttcagttata tactatcttc tagagaatcc
       61 tttacatgtt aagaaagctt cagccattca aggcttccac acaaaggttt attttcaagg
      121 aggttacctt atgaccaaat ttacctggta cggccatgcc accatgggtt tggaaaccgg
      181 cggttataaa ctgttgatcg acccctattt ctctgagaac ccagcagcca ccaccaccgt
      241 ggatcaagtc caggcggact atatcctggt cagccacggg cacttcgacc atgtcaccga
      301 cctactcgaa gtcgccaggc ggaccgaggc gaccatcatc accaatttcg agatctccaa
      361 ctggctgggt cagcagggct tgaattcaca cgcccaacac attggcggcg gtttcaagca
      421 tccattcggc tacctgaagc tgaccctggc cttgcatggc tcgggcttgc cggatggttt
      481 ctatggcggt aatccggctg gtttcttgct caccacgctg gacggcaaga aagtgtattt
      541 ggcgtgcgat actggcctgt ttggcgacat gcgcctgatc ggagaggaag gcgtcgacct
      601 ggcggtgttg ccgattggcg acaacttcac catgggcccc gatgacgcgc tgcgcgccgt
      661 caaattgatc cagccccaac acgtcatccc cgtgcactac aacacctggc cgctcatcgc
      721 ccaggatcct caggcatggg ccagtcgggt gaaggccgag accagcgcgc aggtgcacat
      781 cctcaatcca ggtgagagtt tcaccctacc ctgatcgaga agtatagcca acctgcataa
      841 aaacaagatc gaggtttgta caggttttta caaacctcga tcttgcattg gacctgccct
      901 tcttcttcct attgaccgga gggagcgggc acaaatgagt ttagattctc ttcagtcggt
      961 tatggttttt cattcatctt gatgaactca ccccggcgga tctgaaagtg atcaaaatcc
     1021 cgcgccacgc gggtatgggg gaaaatcgcc tgcgcctcgg ctaacacatc gcgctcacga
     1081 taacgacgcg agatgtgcgt gagaatcaga ttctgcacat ttgcctgttg ggctagctcg
     1141 gctgcctggc gggcagtcag atgggcaaac tcgcctgcca tctcagattc ttcatccagg
     1201 taagtggcct caatgaccag ggcatctgca tcccggcaca cttgcaatag attatctgta
     1261 cggcctgcat ccccgatgtg caccaggcgc gtgccagctc gctctgggcc caatacctgg
     1321 tcgggatgaa tcacgcgccc gtcgggcagg gtcaccacct gtccatcgac caggtcgcgc
     1381 cgccatggac cgggggggat ttcgagtgcc tcagcctttt ccggcaggaa gggacggcgg
     1441 ggtttctcct caaacaaaaa gccaaagcaa tccgggccgc gatggaagac tggtatcgca
     1501 gagacgctaa aatcatcgtc ccgaaagaac tcgcctggct cgatttcccg caagcggata
     1561 tccatgggcg ggcgcgcccc gcgcaacacc accccataca gcaggtcgtg gatgcgctgc
     1621 aaagcccagc ctccagcgta tatctccagt tcatcgatcg cctcccagcg agagaaggtg
     1681 gacagcaggc ccgccagtcc cagaatgtga tccaggtgac cgtgggtgat caggatgcgg
     1741 ctcaggcgtt taaagccgat cccagactgc agtatctggc gctgggttcc ctcaccacag
     1801 tcgatcagaa aacggtattc gtcatgcatc accacctgtg ccgaaaggcc gcggtgcacc
     1861 gagggggcgg acgctgacgt accgaggaaa ataatctcaa acataggctc atctatttta
     1921 gccgcaaagg cacgcttatg gcaagcaata gagcgaccgt gtttcacgca ataatacgac
     1981 catatgtgct ataattttta tcgtcatggc ccgccagacc cgaaaagatg gccgcaaaaa
     2041 gcctgataag tcttccaggc ctgcggtcaa atcccaaaaa tcgaagccaa acccaggccg
     2101 cggcgcctcg aacaaaaaac cggggcgcac cagcaaacca gccgcccggg gcagttcgtc
     2161 gcgccaatcc cgtcaggctc ctggcaaacc agcctcccgt tccaagtcaa cacccagccc
     2221 tcggcagtcc ttcaccagcg gactatccct agatcgcaag ctggatatcg ctggggtagt
     2281 gctatccctg ctgggcttgc ttaccctgct cagcctgatc tccagcagcc gctctgccat
     2341 cacaagcggc tggatcggca tgctcagcaa aggacttgga tggggcattt accttctgcc
     2401 gctgaccttg ctggtggtgg gactttggct ggtgctacgc aacttcgagc aaatcccctt
     2461 gccatcccct gagcgcctgc ttgggctgag cctgctttat ctgaacttgc tgggcatcct
     2521 gcacttgttc aagcttccag agacacgaga agcctccctg gctctggctg atgcagggca
     2581 aggcggaggc tacctgggcg cgctgacttt ggagatattg cgcacgatct taggcatggg
     2641 cggggcaaca gtagccctcc tggcctggtt attaatcgcc atggcgttgt cactggatgt
     2701 ttcggtggta gaacttttcc attggctgcc acccttgctc tcccggctgc aagatcgatg
     2761 gaatgatttc cgtctgcaac gcctgtccaa aggtgtttca caacccgaac tagacggttt
     2821 tacccccctg cagcgcggtg tggatttgga ttacgagctc gatccccaaa ttgctaatcg
     2881 cctggcagcg ttgccatccc tccctcccac cctgggagtc acccccaaag actggcagct
     2941 ccctgtcgtt gaggagatct tagaagccgg aggcgaggtc agctacgaca cggagttgga
     3001 caaacagcgc ggcagggtaa ttgaagagac actagcctcc ttcggagcgc cagccaaagt
     3061 ggtagagatc agccgcggtc ccaccatcac gcagttcggc gtggagcccg attttatcga
     3121 gtcgcgcggc gggcgcatgc gcgtgcgcgt gggcaagatc gctgccctgg ccgacgacct
     3181 ggcgctggct ctttccgctt ccaccatccg catccaggca cccgtacccg gcaagggcta
     3241 cgttggcatc gaagtgccca acgatgaaat aagcctggtg acgctacgca gtgtgatcga
     3301 gaacgaagcc ttccggcggg tcaaatcacc cctgggtttc gccttgggac aggatgtggc
     3361 tggtaacgcc gtcgcggctg acctagctgg catgccacac ctgctggtag ctggggctac
     3421 cggctcgggc aagtcggtat gcgtcaactc gttgatcacc tgcctgctgc tgcacaacac
     3481 cccagaagat gtgcgcttga ttatggtcga tcccaagcgg gttgagctga ccggatataa
     3541 cggcatcccc cacctgctgg cacctgtcgt ggtggagctg gagcgagtgg tgggcgcgct
     3601 gcaatgggtg ctgcgcgaga tggaccagcg ctatcacaag ctggcccagg ctggttgccg
     3661 caacctgaag gaatacaacg ccaagctatt ggcgcggggt gagaagaagc tgccttttct
     3721 ggtggtgttc atcgatgaat tagccgactt gatgatgctt gccccagatg agaccgagcg
     3781 caccatcact cgtctggcac aactggcccg cgccacgggc atccacctgg tgattgccac
     3841 ccagcggccc tcagtggacg tagtcaccgg gctgatcaaa gccaatttcc cggcgcgcat
     3901 tgccttcgcc gtggcctccg gcgtggatag ccgcgtcatc ctcgaccagc caggtgctga
     3961 gcgcttgctg gggcgcggcg acatgctctt tcaagccccc gatgctgccg cgccggttcg
     4021 cttgcaaggt gtcttcgtct cagatagcga aatccagcgt ctggtgcagt actggcagag
     4081 ctacgccggc gcagtagcgc tcgcccccac tgccgttggg ggcgtggtgg atgccgtgcc
     4141 ctcggggttg ccacttaagc aaattccctt atgggatgat ttcactccag aggaggagaa
     4201 agaccccctg ctggacgagg ccatcgacct gacccgccgc cagggccgcg cctccgtctc
     4261 tatgctacag cgccgcttgc gcatcggtta cacgcgcgcc gcccgcctca tcgagagcat
     4321 ggaggctaaa ggtatcgttg gcccgcccga aagcggcaca ggcgcgcgtg aagtgctgga
     4381 ctacggccca gccgcgcctc cgattgatga gcagcgcgaa taggtgctga taacggattt
     4441 ggggaacgga ttacacggat cgtgcattaa agaggtaatc acatggggtc ccaaagccag
     4501 cgtgtcgcaa caccgtatga tgaactgacc tataggatca ttgggtgtgc catggcgata
     4561 catcgcaaac tgggacccgg gtaccgtgaa aacacctatc aaagggacct ggaagcgcac
     4621 ttcactgaca ctgggcttgc ttttgaggga cagaaactac tcgaagtata tgatagctcg
     4681 aagggcgagc aattaatcgg ttactatatc ccggattttc tcgtcgagaa tacagtcgtg
     4741 gtggagatca aggccttgag aggactggat aacagtcatc tcgcccaggt gatcggttac
     4801 ctggccgtga ctggctgccc aattggcctg ctgataaact tcggcacgcg cagcttgcaa
     4861 taccggcgta ttttccctcc aaaggatatc accgaacacc gtgttaatca acaatggttg
     4921 ttcgtgccag attggctcaa aaacaattaa accatcaggc aggtaatccg ttcaatccgc
     4981 tccaaaaatc cgttcacagc catccgttcc cattgtccgt cgccagccat ccgttcaatc
     5041 cgttcccgcc atccgtttac agtaatccgt tatcacgatc tcgtaaataa tccctcgggg
     5101 aagcgtaaag atcacctata aacctcaccc tggagtgaca agtgtgcttc catcaccctc
     5161 cgggcgtgag cccggacagc ctgcatggta tctccatcct tttcaccaag ttgcagctca
     5221 gatagggtga gcggctcggc aaagctcacc cggggcacaa tcgggtaacg ccgccccaag
     5281 acaagctgct ggatcacctg gataaactcc gccagcttct gcttttccca catcttctgg
     5341 cgcaagcgca ccagtgggtg ccgccagcac tcaggagcca gcacaccgct caccgccgtg
     5401 accaacagac gcgtgctggg cgctttctcc agcatgagcg ccagactgga agaccaggct
     5461 tgcagggatc gccacgcgtc ggggaagagg gatggatcgg ggtctacgca gccgctggga
     5521 aaaatcagca ccgccccacc ctggcgtagt tggcggatgg cattgcgcag caccagcatg
     5581 cgcccgtgtg cattctcagc cgtgtaaatc aggtgctcgt ggataaaaga caaattgtgg
     5641 aagaacggta caccgctgac cacgatcttc agatcctgac gcggcaggct ggcagcgatc
     5701 gccaggccgt ctaccgcacc cggatggtta gaggctatca gcagcgggcc ttcggggggg
     5761 atatgctcag caccgcttac caccacatga tggatgaagc actccagcaa gcggcgcgct
     5821 gcttcgctga agccatactc cgcattgtag cgttcgaagc cctccaccag cctagcaaaa
     5881 cggtgggctg gcgcccaagc caaaggccac agagcgcgcc gcagccatcc ttgcggcaaa
     5941 cccatggcat taaaaatctc gtcaatgaga gagcctctta gctccgctac tcgggagacc
     6001 tgtgctcgca t
//
LOCUS       DBNGSX010000020         2039 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1732549, whole genome shotgun sequence.
ACCESSION   DBNGSX010000020 DBNGSX010000000
VERSION     DBNGSX010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1732549"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..567)
                     /locus_tag="AC16CB_00540"
     CDS             complement(22..567)
                     /locus_tag="AC16CB_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340979.1"
                     /translation="MEISPPSLILVAVINDPRDMEIARLLGWYRIPLRSAPKVVAVDF
                     LAFYQTAAFGEEKWRIEFVAPVKGHELTTRAELLRDEPDHPSARHEYYKIQIGPLERL
                     PQPLLAEGWRRITFFYTTGEYFLGAQTIHDLILAEEDRRPLWQALRERASQAQQYAAD
                     IPDVEVDAALLASLLGIQKSR"
     gene            complement(575..2017)
                     /locus_tag="AC16CB_00545"
     CDS             complement(575..2017)
                     /locus_tag="AC16CB_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR3340980.1"
                     /translation="MEKDRRSRAIQRVRERNIIIPTFAQMKNPDKIPARVKEELRNVG
                     LWDLDPRNLFRITWHNEPSPQGGGFGRVNYLEFPKSLTGTEARVIALVGKWFPTGAHK
                     VGAAFGCLVPRLVSGQFEPTTQKSVWPSTGNFCRGGAYDSALLACQSIAILPEGMSRE
                     RFEWLSKIAGEVIATPGSESNVKEIFDKCWELRASGQDLMIFNQFEEFGNYLWHYEIT
                     GHAMQEVLEEVMGPRDAYRGMVSATGSAGTIASGDYMKQLFPASKIGASEALQCPTLL
                     ENGFGSHRIEGIGDKHVPWVHNVKNTDVVVAIDDNAVVNLARLFNEPAGRAYLVKQGV
                     PEALVGKLDLLGFSGISNMLSAIKMAKYYEMGENDIVLTVLTDSMELYGSRLREMHDE
                     YGAYSDLDAAAHFAQYLHGTSTDNMIELTYADRRRVHNLKYFTWVEQQGKTYEEIQAQ
                     WYERDYWTDVQSQVEQIDALIEEFNAEVGL"
BASE COUNT          476 a          591 c          483 g          489 t
ORIGIN      
        1 gtgtcagtgg atgtgcaata gttagcgtga cttttggata cctaacagcg acgccagaag
       61 agctgcatct acctcgacat ccggaatatc cgctgcatat tgttgggctt ggctggcgcg
      121 ctcgcgcagc gcttgccaca acggtcggcg atcctcttca gccaggataa gatcatggat
      181 cgtctgcgca cccaagaaat actcacccgt ggtatagaaa aaggttatcc tgcgccaccc
      241 ctccgccagg agtggctggg gtagcctttc gagagggcct atttggatct tatagtactc
      301 atgacgagcg cttgggtgat ctggttcatc tctcagcaat tctgcacggg tggttaattc
      361 atgccccttg accggtgcca cgaactcgat acgccacttc tcttcaccaa aagcggccgt
      421 ctggtaaaac gccaggaaat ccaccgccac cacctttggc gcgctgcgca agggaatgcg
      481 ataccatccc aacaggcggg cgatctccat atcgcgggga tcgtttatca ccgcgaccaa
      541 aatcagtgac ggtggagata tttccataat ccgatcagag accaacctca gcgttgaact
      601 cctcgatcaa agcatcgatt tgttccacct gggattgcac atcggtccag taatcgcgtt
      661 cataccactg tgcctggatt tcttcatacg tcttaccctg ctgctcaacc caggtgaaat
      721 acttgagatt atgcacgcgt cggcgatcgg cataggtcag ttcgatcatg ttatcggtgg
      781 aagtgccgtg caaatattgg gcaaaatgtg ctgctgcatc tagatcgcta taagcaccgt
      841 actcatcatg catctctcgc aggcggctgc catatagctc cattgaatcg gtcagcacgg
      901 tcaatacgat atcattctca cccatctcgt agtacttggc catcttgatc gccgagagca
      961 tattcgagat gcccgagaag cccaacaagt ccagtttgcc taccagggct tctggcacac
     1021 cctgcttcac caagtaagcc cgcccagccg gctcgttgaa caggcgcgcc aggtttacta
     1081 ccgcgttatc gtcaatcgcc acaaccacat cggtgttctt tacattatgc acccaaggta
     1141 cgtgcttatc tccaatgcct tcgattcgat gagaaccgaa tccgttctcc aatagagtcg
     1201 ggcactgcaa tgcctcgctg gcgccgatct tgctggctgg gaagagttgt ttcatgtaat
     1261 caccactggc aatcgttccc gctgaaccag tggcagagac catgccgcga taggcatcac
     1321 ggggtcccat gacttcctcc aacacctctt gcatggcatg cccagtgatc tcgtagtgcc
     1381 acaaataatt cccaaactcc tcgaactggt tgaagatcat caagtcctgc cccgaggcgc
     1441 gcaattccca gcacttatcg aatatctctt tgacattcga ctcgctgcca ggcgtagcga
     1501 tcacctcacc agcaattttc gagagccatt caaaacgctc gcggctcatc ccctccggca
     1561 atatcgcaat cgattgacaa gccaagaggg ctgagtcata ggccccaccg cggcagaagt
     1621 taccagtaga cggccataca gacttctgtg ttgtgggctc aaactggcca ctgaccaagc
     1681 gtggcaccag gcacccaaat gctgcgccaa ctttatgagc acccgtggga aaccatttcc
     1741 ctaccaaggc aatgacacgc gcctcagttc cggtcaagct cttgggaaac tccaggtaat
     1801 ttacccggcc aaatccacca ccttgcggag atggctcgtt atgccaggtg atacggaaca
     1861 ggttgcgtgg gtctaaatcc cataaaccaa catttcgcag ttcttctttc accctggcag
     1921 ggattttgtc tggatttttc atctgggcaa aagtcggaat aataatattg cgctcacgca
     1981 cgcgttgtat tgcgcgcgat ctgcgatctt tttccattgt aaggtcgatt ttcatttag
//
LOCUS       DBNGSX010000021         2494 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_486159, whole genome shotgun sequence.
ACCESSION   DBNGSX010000021 DBNGSX010000000
VERSION     DBNGSX010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_486159"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="AC16CB_00550"
     CDS             complement(<1..695)
                     /locus_tag="AC16CB_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3340981.1"
                     /translation="MYAVELKGITKRFGEVIANRQIDFSLRQGEIQALLGENGAGKTT
                     LMRILYGLYTADEGQIIVNGQEVEIKSPKDAIRSGIGMVTQHFTLVPTLTVAENVVLG
                     YTRGFRLNMSELQNEVAKAASQFGIELATDALVRHLSVGERQRVEILKSLYRNARVLI
                     LDEPTAVLIPQEVEVLFETLERLKQEGMSIVFISHKLYEVMAICDRITVLRDGQLVGT
                     VEKATTNQAELAR"
     gene            complement(957..2081)
                     /locus_tag="AC16CB_00555"
     CDS             complement(957..2081)
                     /locus_tag="AC16CB_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HIR3340982.1"
                     /translation="MKKRNVLYSVVLLVFLTSLVLVSCQAEPQTIIQTVEVPGEEVVV
                     TVEVPVIETVEVEVEVPVMPTEEAVRKLKVFGAFATPIEEPWDGVIHSALNKAQDEGA
                     IEYEYTENIGYAGDMERILREVSEQSKPDIIFGDAFGNEEAVRRVAAEYPDIAFVFGS
                     GLGPSNPNVSVFDNWIHEPAYLCGMLAGGLTKTNTIGVVGGYPVPEVNRLVNAFIAGA
                     KEVNPDAKVLVTFINSWFDPAAAKEAALAQIDSGADVLYAERFGVIEAASENGLWAFG
                     NMSDQNSLAPEIVVSSPVWNMNPTVEFIINQVQTGAYTAIDLKDFSMVAKGGASLAPF
                     HDTESKLPPDLVQKVREREQQIISGLFRVDIDEAQPGPVN"
     gene            complement(2147..>2494)
                     /locus_tag="AC16CB_00560"
     CDS             complement(2147..>2494)
                     /locus_tag="AC16CB_00560"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HIR3340983.1"
                     /translation="VEGAMIMALGATLMEKLHFDVDGRIQNHNLVDYRIITTTDTPRM
                     EVIFIETEDASGPFGARGLGEHGIVAIPPAIANALADALGVEFREMPVTSEMVFEALE
                     ARREGGSIEDYVV"
BASE COUNT          570 a          733 c          585 g          606 t
ORIGIN      
        1 atgcgcgcca gctcagcctg gttggtggtc gccttttcca ccgttccaac caattgaccg
       61 tctcgtaata cagtgatccg gtcgcagatc gccatcactt catacaactt atgactgatg
      121 aagacaatcg acatcccctc ttgcttcaac cgttctaggg tctcaaacaa aacctcaact
      181 tcttgcggaa taagcacagc agtgggttca tccaggatca agacacgcgc attccggtac
      241 aaggacttga gtatctccac cctttgccgc tcaccgaccg ataggtgcct caccagggca
      301 tctgttgcca gctcaatgcc gaactggctg gcagccttag caacttcgtt ctgcagttct
      361 gacatattga gtcgaaaacc gcgcgtgtat cccaggacta cattctcagc tacagtgagc
      421 gttggaacca aagtaaagtg ttgtgtgacc ataccgatgc cagatcgaat ggcatctttg
      481 ggagatttta tctccacctc ctgtccattg acaatgatct ggccctcgtc agcggtatat
      541 agcccataaa ggatgcgcat tagggtcgtt tttcccgcgc cgttctcacc caacaaggct
      601 tgaatctcgc cctggcgcaa ggaaaaatca atctggcgat tggcgatcac ctctccaaac
      661 cgcttagtga tgccctttaa ttccacagca tacataatga gtcctgttgg tagggctgcc
      721 caaaaaggtc aaaccttcaa tatgcaggtc gagtcgtcaa ctcgtacact accgcttttt
      781 ggacagcccc aaaagtggct cgggtcatcg aagatctgac cggtcttggg tcagacaacc
      841 cgatttccca atttgaaata aaatccagtt atttttgttg caaaacccag catttacccc
      901 aaccgcagtg taacttcggt tggagcggca attgcactgc cgcatcgcac caaaatctag
      961 ttcactggac ctggctgagc ctcgtctata tcaactcgga agaggccgct gatgatttgt
     1021 tgctctcttt cccggacttt ttgaaccagg tcagggggaa gcttgctctc cgtgtcgtgg
     1081 aaaggagcca ggctggctcc gcccttggcg accatgctga aatcttttag atcgatggct
     1141 gtgtaagcgc cagtctgcac ctggttgatg atgaattcta cagtggggtt catattccag
     1201 accgggctgc tgaccacaat ttctggggcc agcgagttct ggtcgctcat atttccgaac
     1261 gcccatagcc cgttttcact ggctgcttcg atcacgccaa acctttcggc ataaagcaca
     1321 tcagccccgc tgtcaatctg agccagggcg gcctccttgg cggcagctgg gtcaaaccag
     1381 ctattgatga aagtgaccag caccttggcg tctggattca cctccttggc acccgcgatg
     1441 aaggcattga ccaggcggtt gacctcaggt acgggatagc caccgaccac accaatggta
     1501 ttggttttgg tcaagcctcc cgccagcatt ccgcacaaat aggctggctc gtgaatccag
     1561 ttgtcgaaga ctgacacatt ggggtttgac gggcccaagc cagagccgaa cacgaaggca
     1621 atatccgggt attcagccgc cacccgccgg acagcctcct cgttgccaaa ggcatcaccg
     1681 aagatgatat ccggcttcga ctgttcggag acttcacgca aaatgcgctc catgtcgccc
     1741 gcgtaaccga tgttctcggt gtattcatat tcgatggccc cttcgtcttg ggctttgttg
     1801 agcgctgagt ggatcacgcc gtcccagggc tcctcaatgg gtgtggcaaa ggcaccgaac
     1861 accttcaact tgcggacagc ctcctctgtc ggcatgacag gcacctccac ctccacctcc
     1921 acggtctcga ttacgggtac ttccacagtg acgacaactt cctcgcctgg aacctcgacg
     1981 gtttggatga tcgtctgtgg ctcggcctgg cagctcacca gaaccaggct ggtcaggaaa
     2041 accagcagga caaccgaata tagaacattt cgctttttca tactctccaa ctcctcagca
     2101 tttaggcttg tctgataatt aaacaggcta attggttgat ccgattttat acaacataat
     2161 cctcaatact acctccttct ctcctagctt ccagtgcctc aaaaaccatc tcggatgtga
     2221 caggcatctc cctgaattcc acacccagtg catccgccag agcgttagca atcgctggcg
     2281 ggatagccac gataccgtgc tcgcctaatc cgcgtgctcc aaagggtcca gatgcgtctt
     2341 ccgtctcgat aaagatcacc tccattctgg gggtatccgt cgtggtgata atccggtagt
     2401 ctaccaggtt atgattctgg atgcgaccat ccacatcgaa atgcagcttc tccatcaatg
     2461 tcgcgcccag ggccatgatc attgctccct ccac
//
LOCUS       DBNGSX010000022         1753 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1871267, whole genome shotgun sequence.
ACCESSION   DBNGSX010000022 DBNGSX010000000
VERSION     DBNGSX010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1871267"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..619
                     /locus_tag="AC16CB_00565"
     CDS             134..619
                     /locus_tag="AC16CB_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HIR3340984.1"
                     /translation="MNITSVKIEKPDELNFILGQSHFIKTVEDVHEALVAAVPGIKFG
                     LAFCEASGKCLVRWSGTDQAMIELAQANALRIGAGHSFIVFLGEGFYPINVLNIVKMI
                     PEVCRIFCATANPTEVLIAETDQGRGILGVVDGAIPNGVEGDEDIAWRKDFLRMIGYK
                     L"
     gene            629..>1753
                     /locus_tag="AC16CB_00570"
     CDS             629..>1753
                     /locus_tag="AC16CB_00570"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HIR3340985.1"
                     /translation="MRLSPAQLDLIQSPLDAKIFLHGPAGCGKTTAAVERLLYLMEQG
                     VPGGSILLLLPQRTLAQPYNEALRTPGVVAGSMVMPQTLGGLAQRSVDLFWPLVAEEA
                     GFARPDRPPTFLTLETAQYYMAHLVRPLREEGGYFDSVSIDPNRLYSQILDNLNKAAV
                     VGFPLTEIGERLKASWVGEPGQVRIYDDAQACALHFRQYCLAHNLLDFSLQLEIFLRH
                     LWPLSVCRQLLMDTYQHLIFDNVEEDTPVTHDLVGEWLPHFTSALLVCDQDAGYRRFL
                     GADPQSASALSDLCQPVLFDESYVLPPELQAFGESVSHWLAPAEPGTRKAIVNSSAES
                     EVDTVLFAEYRRFYPEMLDLVSDQIAALVDGEGVPPSEIVV"
BASE COUNT          340 a          479 c          516 g          418 t
ORIGIN      
        1 caaaaagggc tcttcttcgg gataatcgct ttcgttattt acgtctgtcg gcatgttgac
       61 tcctttaatg gttcgaattg tactatataa tacgttttat gagcccgatc ctgaccagtg
      121 aaaggaaacc aatatgaata tcaccagtgt gaagatcgaa aaacccgatg agctcaactt
      181 catcctgggt cagtcgcact ttatcaaaac tgtggaggat gtgcacgagg cgctggtcgc
      241 ggctgtcccc gggataaagt tcgggctggc tttttgcgag gcttcgggta agtgcctggt
      301 gcgctggagc ggtaccgacc aggcgatgat cgagctggcc caggcgaacg cgctgcgcat
      361 tggcgccggg catagcttta tcgtgttcct gggtgaaggg ttttacccga tcaacgtcct
      421 gaatatcgtc aaaatgattc cagaggtctg ccgcatcttc tgcgctacgg ctaaccccac
      481 cgaggtgctc atcgctgaaa cggaccaggg gcggggcatt ctgggtgtgg tggacggcgc
      541 catacctaac ggtgttgagg gtgacgaaga tatcgcctgg cgcaaggatt ttttacgcat
      601 gattggttat aagttataga gggatacctt gcgcctttct cctgctcagc tcgatctcat
      661 ccaaagccct ttggatgcca aaatcttctt gcacgggccg gcgggctgtg gcaaaaccac
      721 tgctgcggtg gagcgcctgc tctacttgat ggagcagggc gtcccgggcg ggtcaatcct
      781 gctcctgctc ccacagcgca ccctggctca gccttataat gaggccctgc gtacccccgg
      841 tgtcgtggcg ggcagcatgg tcatgccgca gacattaggc gggctggcgc agcgcagcgt
      901 ggatctcttt tggccattgg tggctgaaga ggccggcttc gcccggcctg accggccacc
      961 tacattttta accctggaga cggcgcaata ttatatggcg cacctggtcc gtccgctgcg
     1021 agaagagggg ggctattttg actcggtctc tatcgaccca aaccgtttgt acagccagat
     1081 tttggacaac ctcaacaaag cggctgtggt gggcttcccg ctcactgaga tcggggagcg
     1141 cctcaaagct tcctgggtcg gcgagccagg acaggtgcgg atctatgacg acgcgcaggc
     1201 ctgtgcgctc cattttcgcc agtattgcct ggctcacaac ctactggatt tctccctgca
     1261 gttggagatc tttttgcggc acttgtggcc cctgtctgta tgccgccagc tcttaatgga
     1321 tacctaccag cacctgatct tcgacaacgt ggaggaagat acgccggtca cacatgacct
     1381 ggtcggtgaa tggctgccac atttcacttc ggctttgctg gtctgcgatc aggacgcagg
     1441 ctaccgccgt ttcttgggcg ccgacccgca gagcgcttct gcgctcagtg acctgtgcca
     1501 gcctgtgctg ttcgatgaat cctacgtctt gccgccggag ctacaggctt tcggagagag
     1561 cgtgtcacac tggttggctc ccgcagagcc tgggacgaga aaggccatag taaatagcag
     1621 tgcagagtca gaagtcgaca cggtgctgtt cgctgagtac aggcgctttt atcccgagat
     1681 gcttgacctg gtctctgacc agatcgctgc cctagtggat ggggaaggtg ttccccccag
     1741 cgagattgtc gtg
//
LOCUS       DBNGSX010000023        25031 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_70522, whole genome shotgun sequence.
ACCESSION   DBNGSX010000023 DBNGSX010000000
VERSION     DBNGSX010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 25031)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 25031)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25031
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_70522"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..161)
                     /locus_tag="AC16CB_00575"
     tRNA            complement(88..161)
                     /locus_tag="AC16CB_00575"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(125..127),aa:Asn,seq:gtt)
     gene            388..1209
                     /locus_tag="AC16CB_00580"
     CDS             388..1209
                     /locus_tag="AC16CB_00580"
                     /inference="COORDINATES: protein motif:HMM:NF025690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HIR3340986.1"
                     /translation="MALKGNLRDFSITQLLNLINLAQKTGTLVVESPKLAAWVAFRDG
                     KLAYAQIGQDDNSLATILYRTKKLSAAQHRIIKERAPQMSDKELGLLLINASYLTQQD
                     ILASLQGHFTGVVQQLFTWGEGFFRFDNDILPPNGKIMVRVNLENIILEGSRKLREYE
                     QLQDEIPSLDMALKFADRPGTNLRNLSLSADEWRVISLINPKNSIRQIAAAANKNDLE
                     IRRIVYGFLQAGIVELIRPAGAPPIPAMQRQFTQQGSKTEQKSLINRIISRIRSL"
     gene            1283..1822
                     /locus_tag="AC16CB_00585"
     CDS             1283..1822
                     /locus_tag="AC16CB_00585"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR3340987.1"
                     /translation="MQTVKMVVTGPFNAGKTEFIRSVSEIDVVSTEKKISSRAERVKE
                     TTTVAMDFGRITVDDDLVLYLFGTPGQKRFDFMWEILSEGMLGFVVMIDSTRPETFRE
                     ARSILETFRAYAPTPYVVTANKQDSDESWDLEDMRIALRLSPEVKLLPCVALDREAVK
                     TVLLELLYSILAEMDEEKS"
     gene            1832..2629
                     /locus_tag="AC16CB_00590"
     CDS             1832..2629
                     /locus_tag="AC16CB_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3340988.1"
                     /translation="MSSITTDQGIVHYEVFGRGRPVILLHGWLGSWGLWQDTMTWLGR
                     YYRTYALDFWGFGESGKKRDSYAVQDFVSLVDQFMEQLGIAQAPLVGHSMGGTVSLSV
                     AIQYPQRVSNVVVIGSPINGSSLNFLLKLFGYRLVAYIVHNNLWILRLGFRLLAPLYT
                     RDKRWPEMMDRDLSKTTLESFLMSIATLRRTDLRPSLDRIRVPVMGMYGDKDIVVDPG
                     QWKPLLDGAPHTRVERFKDAGHFIMLDEPKKFVETIQDFLSCETSSP"
     gene            2650..3261
                     /locus_tag="AC16CB_00595"
     CDS             2650..3261
                     /locus_tag="AC16CB_00595"
                     /inference="COORDINATES: protein motif:HMM:NF014845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V4R domain-containing protein"
                     /protein_id="HIR3340989.1"
                     /translation="MHNYSNKMGRIVLVAIEEILGRNGINATLNLAGLSHYINSYPPN
                     NLDLQFNFDDLVEIQVALEELYGPLGGNGIALRSGRASFKYFLREFGPPLGITDVSFR
                     LLPLNEKLSTGAQLFAEIFNRYSDQRVRVEDTKERVYWHVERCPICWGRQTDHPVCHF
                     IVGFLQETLYWVSAGKYFIVEETKCVAMGDPGCTIEINKQPVD"
     gene            3262..4539
                     /locus_tag="AC16CB_00600"
     CDS             3262..4539
                     /locus_tag="AC16CB_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012360.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HIR3340990.1"
                     /translation="MLKIILQSPQYIQPFNERARDLRVHNKPLWLFQRDVLSIYTHRE
                     LELPPRAPLPNTSEECLVYRDNLFFDAPYIDAFIKEARRRGRPCQAAFSASDLAFREH
                     ALPLSVSYTPMDDLYLAELWYYPQGVQLESEPLVIDLGASEVGYYHVPIYMAFEQGDL
                     VFQVPRRGLLAIDSWVHIFIADVVFGVFSRGARFEDRLKDDPLFKLRILARAMYEGKQ
                     VLQSSELVKVGRNCVIDPHAVIHGPTTIGDNVTINAGSVVENCIIGDNVNISQGCHLM
                     LSVVGDGAFLPFRSSLFMTTLMDNSMVAQNTCLQMSVIGRNTFIGAGSTFTDFNLLSI
                     PLRALDGNSLLNHANRPVLGGCVGHNCRIGSGMIVYPARTIESDVVLLATSERYVITQ
                     DVRYEDSDHHRLPFGEVHPRLYPRQAEAASESW"
     gene            4637..5524
                     /locus_tag="AC16CB_00605"
     CDS             4637..5524
                     /locus_tag="AC16CB_00605"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HIR3340991.1"
                     /translation="MAKGRLLIVEDDTDISTMLRIFFNGLNYEVDIAARGSDALEKTR
                     QNLPHLIVLDIMLPDIDGFEVCRVLRTNTRTSHVPIIFLTQKDERSDKLQGLELGADD
                     YITKPFDIEELKLRVQRAIQRADQQSLTDPRSGLPSGKLIEEQLRRIIRLRGWAMMDI
                     RIDHFEAFKEVYGFVAGDDVLRFGAMLMGEVVDEFGSPGDFIGHAGGDNFLLICAEEN
                     ASAIRQALKTRFANEVLSHYNFMDREQGYIVTYNEAGQPVQSPMMTLSIGLVSPSQQQ
                     FADIREITELAAEARRSEG"
     gene            5548..12630
                     /locus_tag="AC16CB_00610"
     CDS             5548..12630
                     /locus_tag="AC16CB_00610"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3340992.1"
                     /translation="MSSTGLGIVLLVLGLGVFALTLFLARSVPRNRDASQAASMPAML
                     LEISRHSEATFLVQSGGRLVHYNSAASELFNLEAEPNLNELGRRARPSEVFWGLCAAE
                     GQARFLLDGRVIEGISYAVPYGSGQAILVTLRRPKLLTSHRDEKASEDGSSHQYQGLD
                     ILAEVSQAMTSSLNLEATLRSIIESIEYVISCDRCEVNIWDADNRYLVPHSLTGTVQM
                     QRKLQTSDRRNLPNQGYTGYLVFHRKPLFISDVDNFIQARPAGDKADFPFHSYVGLPL
                     EAGGELVGTLELASLEKDGFTESDLELLRMLSRQAALALHNALSYEAQAKRAGELAGL
                     AQLAQVITAQQDPQDLYSRLVESIAPLLNVKILGFWVMDESQHALVGQTPFLGTQNSV
                     VEWTKVILQPGSQAEKLWLTQESIVSDDASSDVRIQHLGLEHFAVAAGVRQTVLVPLT
                     SSGHMLGYLQAANKRDGSDFDQSDLRLLAIIAGQAAPIIENAGLVHESSRRAQRAEML
                     RRIANLTASSAKLDEILKLSLQELAQLLGADMALMLLFDEGKGELRLHRPSLFGISPD
                     VTLRLGRLPVDDPEFPNTATGSLKSLLVGDVMDEPDVISTYRNTAINLQGRSILVTPM
                     VLRKRGIGELLLIGRDVASFNPAELQVVAAAADQLAGAIERADLYAQTDESLRRRVDQ
                     LTAVTRVSRELSSTLQLEQLLQRVFEEVLRTTRADCGTIVLFALHEADFQNNVSSSSP
                     RVLLRLGESKSGLHSLERMVLKRGETLILNDFNPLRDVVDALEAGIELAQPPHEGIQS
                     AMVIPIFYQDQIAGLIHLHARAPFRFDESAREIGETLAIQASIAIGNAQRYQEQYHRS
                     ELLDRRMGTIAKLLETSQAWQLESPLEKSLEEIASAIQGATPFDVVVISIYNPDDQKL
                     HRLANAGLSLTDMEELRAHPQSWGKLQDVLKPEFQIGRSYFIPVEQMPVMPPDVHSLI
                     IQPVEDAATDEADHPGRVWHPEDALVVPLHSAQGEPLGLISVDLPRDHLRPDLPTIET
                     LEIFASHARLVIESQREYSRLQQQLDTIQHELQLSKEVAETAQANLPVLLQKDIDQSL
                     ALRRLGQHARRIRAGIDIADLANSQSDRSSVLRTLGNEFLTRLNLDVVLVVEPGLGGP
                     HLLEALGELPSEINLEALIGQRNPLYHCLRTQDQLLVDRLESKMKWADAPLLQALDVQ
                     SFICFPILSGDRLDAAVLGVSRSPMDPFTEDDEQIFTLLSRQVGITIQNLQLLGETSQ
                     RLAEVNLLLDFNRRLGGLNPTGIAHTLGQSILQLVPNSQAVLVALWDTQRRALVPQDA
                     IGYADATRMMEIIYNPGEALPGQVFESGQAVILDEVDFAKHYSLTSENLLRFRNATGG
                     KLPVSTIVAPILPAEGAQALGIVVVDNFQMSAAFTPEILALVTSLAQQAALNLENTRL
                     VQAAEERASQLQALTGVAATITSNLQPDNLIAMLLSLLGAILPYDTGTLWLKQDENEM
                     IVRSAHGFSDNEQRIGLTVALQDSILLSDMIASGQPVAVGDIRHDARFSSLIEPQYIS
                     WLGVPLITSGEVIGVIALEKSESNYYTQEHRQIATTFASQAAVALENARLYHESLNRT
                     LELDQRSQRLEMLNRLSAALIATLEPEHILQVATQELGRAIQCSGVSAVLFDADGVGK
                     LHAESPQVEDVLPCALPDVPLFERLRQTQGVFNAGDIGVESELAPLSEFLEARQARSL
                     MALTLATGEDTQGILLAYADRESRFTPDEVGLARTIANQVSIAIQNARLYAETRSLSQ
                     DLEQRVIERTAQLEKAQQRAETLLRINMELSTSLDLEQVLNQSLHLVNQIVDAGQVSV
                     LITRSGEKKFYRLASRGYAGSPDSDGQTTLINVDEGLAGWVIKARQTVLVDDVLQDPR
                     WLKLTDAPEPHHRSALAVPLLRGAECVGVLLLYHQDVGHFSDDHLDLIQAVANQVAVA
                     VNNAELYRLIRDQAEDLGQMFRRQQVEASRARAILEAVADGVLVTDAKRNITLFNAPA
                     EEILGLDRSQVIGHSLDHFTGLFGRAAQRWNETIRTWTQDPSTYEQGELYAEQIDLDD
                     GRVVSVHLSPVIMEDNFLGTVSTFRDITHQVQLDRLKSEFVATVSHELRTPMTSIKGY
                     VDVLLMGAAGSLTEQQVEFLDIVRDNTERLTVLVNDLLDISRIESGQVTLSMLPLNLD
                     EIITQGAAALRTRAESDQKPMDIQFAPASELPLAMGDPERVRLILDNLLENAYYYTAP
                     KGRITVQVHATGDEIQIDVQDTGIGIPEEAQARVFERFFRGEHPFVLSTSGTGLGLSI
                     VQHLVEMHNGRIWVESSGVPGEGSTFSFTLPAFQHDQAGSQKFHDEQVRQLAKE"
     gene            12685..13077
                     /locus_tag="AC16CB_00615"
     CDS             12685..13077
                     /locus_tag="AC16CB_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3340993.1"
                     /translation="MDHKNMARILIAEDEKDIRELITFTLSFAGHQVTATTNGEEAYL
                     TAQEMLPDLILLDVRMPRMTGFEACEKIKANEKTRQIPVVFLSAKGQEVEIQTGLNLG
                     AVEYIVKPFAPEQLAERVGAVLANFGNG"
     gene            13107..13895
                     /locus_tag="AC16CB_00620"
     CDS             13107..13895
                     /locus_tag="AC16CB_00620"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3340994.1"
                     /translation="MSAVILQDEIVHYEVLGRGRPVFFLHDWIGSWRYWIPSMQATSL
                     SFRAYALDLWGFGDTSKNPSYYSIEQQVGLLEEFVNNLGIGKIALVGHGLGAVIAMLY
                     ASRHPEGVDRVMTIGMPFGEDRINSRLRLNSPSELASWLLGRSPAAEAAYVDAPKADH
                     KAILLSLTSLQGVELACLPHQLATACLLVHGGNDPLIETPNSNHVEELPEESQFIIFE
                     KSGHFPMLDEPGQFNRLLVDFLSLESGQSPRQLQLKEEWKRRVR"
     gene            14338..14790
                     /locus_tag="AC16CB_00625"
     CDS             14338..14790
                     /locus_tag="AC16CB_00625"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR3340995.1"
                     /translation="MTSKADVIRRWHKDSWANPPSSIGEAATKYFSDDYQGLDKDGNV
                     MSDKAAYIGMSQLLLTAFIGFKGVVHDISEEGDSVILTFHFEGTHTGDLDLSMMGLGV
                     IPASGKRIVTPESKTRFKVEGNQIVGSQPISGGFESLLAGLGVKPPPG"
     gene            14997..15527
                     /locus_tag="AC16CB_00630"
     CDS             14997..15527
                     /locus_tag="AC16CB_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HIR3340996.1"
                     /translation="MSKSTKAIALMLVLAFTLSVFVSGALAGPKGPTIVDVAIAVNSD
                     GPFAGQFDTLIAAVLAADPAVLNTLSGNGQFTVFAPTDAAFAALELTPDNIGTLPQAF
                     LTDVLLYHVAHGSRYAEDVVDSKRIRTLNKSFLLQDGGVLTDNLGREATIIVTDVPAA
                     NGVIHAIDAVVLPYAP"
     regulatory      15867..15978
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            16015..16674
                     /locus_tag="AC16CB_00635"
     CDS             16015..16674
                     /locus_tag="AC16CB_00635"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HIR3340997.1"
                     /translation="MTTPTRAVIFANGVLNHPETALAALRPGDLIIAADGGARLCRQL
                     GLVPAILIGDFDSIDEADLAYFDANGTQIIRHPARKDYTDLELALRHVKSLGIQDVLV
                     VAALGARWDQTLANLLLPAAVDLGDLTIRLMDGPQEVVLLRGGDEIDIHGKPGDTVSI
                     IPVGGDVHGVVTDGLEYPLFNESLFFGATRGLSNVLLVGRATIHLEQGLLLCVVIHQD
                     G"
     gene            16735..17916
                     /locus_tag="AC16CB_00640"
     CDS             16735..17916
                     /locus_tag="AC16CB_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3340998.1"
                     /translation="MFKNISLIRSNCRYAPPEGRRGNLLLDFFAWPQKCWPLSILLLF
                     LILGLSACAPASLAPTSTGELPTVTPSGPRTLTVMTHDSFAVSDEVILAFESAYSVAV
                     KILPSGDTGTALNKAILSKGNPIADVFYGVDNTFLSRALDEDIFEPYDAPGLADVPEA
                     FLLDPGKRALPVDYGDVCPNYDKTYFSEKGLAPPRTLEDLAKAEYKGLLVVENPATSS
                     PGLAFLLATIGRFGADGYLDYWKSLVENDVLVVNDWESAYYTEFSAHGGTRPIVISYG
                     SSPPAEVIFAESPIEESPTAVIAAGRSCFRQIEFAGILKGSQNRDLAELWMDYMLSKT
                     FQEDMPLQMFVFPVSRQAVLPDEFAKYTSIPADTAQVNPDEIAANREQWLNSWTEAVL
                     R"
     gene            17930..18076
                     /locus_tag="AC16CB_00645"
     CDS             17930..18076
                     /locus_tag="AC16CB_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340999.1"
                     /translation="MKFRTVYFLWLAPIAFLAIFFFYPLGSILAVSFGRALGEDMLEL
                     PRAL"
     gene            18098..19639
                     /locus_tag="AC16CB_00650"
     CDS             18098..19639
                     /locus_tag="AC16CB_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3341000.1"
                     /translation="MAFTFGQATLSTLLTLLMGLPGAYLIARYEFRGKSLFQALSGIP
                     FVLPTLVVAAAFNALLGPRGWINLALMDWFNLTQPPIQFLNTFTAIIVAHVFYNLTIV
                     LRIVGDFWSHLDPRLVQASQMLGASRLQTLLRITLPLLAPAIAAAALLVFIFDFTSFG
                     VILILGGPRFATLEVEIYYQAISLFNLPMAATLALLQLVCTLAMTTIYTRLAAGLARP
                     LGLRPRRFTQRRLVTWRSRLVAAMLVGLMSVFLITPLAALATRSFTRVDAGSQVGGSA
                     GDITLEWTLDYYRELSINRRGSLFFAPPSTAIAVSLGYAAATVVLALLLGLPASWALA
                     RYQSSALSRLLDPILMLPLGTSAVTLGLGFIVALDQPPLDLRASPVLVPLAHTMVAFP
                     FVVRSLTPSLRSIRPRLRQAASVLGASPSQVVRTIDLPMVGRALLVAATFAFTISIGE
                     FGATALIARPEYPTIPIAIYRYLGMPGALNYGQALALSTILMGVTAAGMLAIERFRIA
                     EVGEF"
     gene            19642..20691
                     /locus_tag="AC16CB_00655"
     CDS             19642..20691
                     /locus_tag="AC16CB_00655"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018839603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3341001.1"
                     /translation="MNTKLGLGVQGIHKTFGEIRAVSGVSFDVDPGEVMALLGPSGCG
                     KSTVLAVIAGLEQADAGGISWDGKSLREVPPHERGFGLMFQDYALFPHMNVFGNIVYG
                     LRMRGWPEDRLRKRVGDMLELVGLPGFDKRDVNTLSGGEQQRVALARSLAPEPRLLML
                     DEPLGSLDRNLRERLVSDLRQILKRMHQTAIYVTHDQEEAFALADRVAVMNAGRLEQL
                     DAPQMIYARPASPFVARFLGMTNLLDGVIRPTLDGIVVETAVGAFPVVGQFQGRVTLL
                     LRPDAVRPDGVGSMHLQGRLLGCSFRGSSCQATIEVDGVPLKFDFLTSTPLPPAGELV
                     TLGFEPGDALQVFSQ"
     gene            20775..21302
                     /locus_tag="AC16CB_00660"
     CDS             20775..21302
                     /locus_tag="AC16CB_00660"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: protein motif:HMM:NF016085.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016891 - RNA endonuclease activity
                     producing 5'-phosphomonoesters, hydrolytic mechanism
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease FAU-1 family protein"
                     /protein_id="HIR3341002.1"
                     /translation="MSSITVIKLDINGVETWRYPGQLLHKQDTQIVLEAYFDRDDMIF
                     HGMPLHRGDRFLELYFTDRWYNIYEIHSRADDHLRGWYCNVCTPAQLDGDRLFFKDLA
                     LDLLVFPDGNQIVLDEDEFARLELSPDGHRLALAALDELRAGFKQPAWLEALGNPGYL
                     TIPRANLPGKNRIGE"
     gene            complement(21452..22012)
                     /locus_tag="AC16CB_00665"
     CDS             complement(21452..22012)
                     /locus_tag="AC16CB_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014694373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HIR3341003.1"
                     /translation="MTGVFSAFGLSASAGLNAYIPLLVIALLARFTDLIELSSPWNAL
                     ESWWIIGLLVFLSLVEFFADKIPAVNHINDAIQTIVRPVAGALAFAASAKVITDVNPI
                     LSMAAGVLVAGGVHAVKSLAVRPAVTITTGGAGNVPVSMLEDFISTVLSILSIVIPIL
                     IGSVLILFTAWVVWRMWRRTNSISTA"
     gene            complement(22272..23090)
                     /locus_tag="AC16CB_00670"
     CDS             complement(22272..23090)
                     /locus_tag="AC16CB_00670"
                     /inference="COORDINATES: protein motif:HMM:NF021384.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HIR3341004.1"
                     /translation="MADAREKLHTNSQTLKLLVFVAVGFLFLGWLFNTPPGLLGKADA
                     IGYAVCHRIDLRSFHLGDRTLPLCARCTGMHLGALLGLVYQSAFSRRKAGLPPIRVLV
                     VLGLLASAFFLDGGNSFLSLIPGAPTLYTPQNWLRLFTGTSMGVVMAALLFPAFNQTT
                     WRYWQPQPALSGVRQLAWLVLLAAILDLVVLTQNPLILYPLALLSAIAVLVELTLVYS
                     MVSMMIFRLENTYTKLAKMMLPLTAGFGLGLAQIALLDIIRYLLTGTWDGFHLG"
     gene            23250..24452
                     /locus_tag="AC16CB_00675"
     CDS             23250..24452
                     /locus_tag="AC16CB_00675"
                     /inference="COORDINATES: protein motif:HMM:NF022702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HIR3341005.1"
                     /translation="MMNKRWTLVGLMVVALSACSTFAAPAPTPTTEATSTPSLAPSET
                     PTVTPTVQPTPTATETPSPPTATPLPQDVGPANFPQGVNPLTGLTLADPALLERRPLA
                     VKIQMFPRGQRPPWGLSLADIVYDYYQNDGMTRLHALFYGNDAEIVGPIRSARLLDNR
                     LVRMYRSIFAFGGADQRILNVLLNSEYYDRLIVQGSSSPMWREDPNGANYLLTGIQQM
                     AEYINSKGISNSRQNLDGMRFELQPPAGGGSASQVFVRYSISAYNRWDYDPTTGSYLR
                     YVDTQEDTGQGESYEQLVDRLTEQPISAQNVVVVFAVHDDYLRNGRYNVVDILLDVSG
                     PAFIFRDGQAYEVRWYRPQLESVLSLLDLNGNPFPFKQGNTWFQVIGQSSRWGNESGV
                     WRFESRIP"
     gene            24484..>25031
                     /locus_tag="AC16CB_00680"
     CDS             24484..>25031
                     /locus_tag="AC16CB_00680"
                     /inference="COORDINATES: protein motif:HMM:NF022702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HIR3341006.1"
                     /translation="MKTGLSSIAVLWALMISGCAAVALDPQVSVSSTPPAEVSEIATE
                     EPSAALEVIATIPIATETATPSVTLEPPTTATSFVYAYGPSDFPEPINPLTGLPVDPP
                     QLLERRPIVIKVTNFPRSVRPQWGLSQADHVYEYYIGDSMSRFVGVFYGKDAERVGPI
                     RSARLFDAHVMRMYHGIFIFGWA"
BASE COUNT         5565 a         6500 c         6863 g         6103 t
ORIGIN      
        1 aagaattctt caccaggcag ggataacgaa gtaatttaaa cgaaaacgca cccaggtctg
       61 ggtgcggatc gtttctcatc caagaggctc ctcgggtagg actcgaacct acaacctagc
      121 ggttaacagc cgcccgctct gccgaattga gctaccgagg aatttggcga cgtggattat
      181 aaacttaagg ggtgggggtg tcaagcgaat gagttagacg ccagcacatc gcacttcacc
      241 ttaccttaca acttcatcag ttctcctttg atgggctatt ctaataatgt ataatcgctt
      301 agcgttaacc tgtctagaag gtgtctcaaa aatccttcca accatttttg agatgaattc
      361 tgaccatttt ctcttggtga agctgatatg gcattgaagg gtaaccttcg cgatttttcg
      421 atcacgcaat tgctcaacct gatcaacctg gcccagaaga cgggtacgtt ggtcgtcgaa
      481 agccccaagc tggctgcctg ggtcgccttt cgcgacggga agctggcgta tgcgcagata
      541 gggcaggacg acaacagcct ggcgaccatc ctgtaccgca ccaaaaagct cagcgctgcc
      601 cagcaccgca tcatcaagga gcgcgctccc caaatgagcg ataaagagct ggggctgctg
      661 ctgatcaacg ccagttacct tactcagcag gatatactcg ccagcctgca aggccatttt
      721 accggagttg tacagcagct cttcacctgg ggagagggat ttttccgctt tgataacgat
      781 attctccctc ctaacgggaa aatcatggta cgggttaacc tggagaatat catcctcgaa
      841 ggctcgcgca agctgcgcga atacgaacaa cttcaggatg agatccccag cctggatatg
      901 gctttgaaat ttgccgatcg accgggcact aatctgcgca acctgagcct gagcgcggat
      961 gaatggcggg tcatttcgct gatcaatccc aagaattcca tccggcagat cgcagcagca
     1021 gccaataaga acgacctgga aatccgccgc attgtgtatg gttttttgca ggctggtatt
     1081 gtggagctta tccgcccggc gggggcaccg ccaatacccg ccatgcagag gcagttcacc
     1141 cagcagggca gtaaaacgga gcaaaaatcg ctcatcaacc gcatcattag tcgtatccgt
     1201 tctttgtagc ggatgaaaag gacgtgggaa tcccagcgcc aatcagcgct ggggcgaaaa
     1261 ttcctcaaat gaggatgggt acatgcaaac ggtaaaaatg gtggtcactg gccccttcaa
     1321 cgcaggtaaa accgagttca tccgctccgt gagcgagatt gatgtggttt ctacggagaa
     1381 aaaaatctcg tcacgagccg agcgagtgaa agagaccacc acagttgcca tggatttcgg
     1441 gcgcatcacg gtcgatgatg acctggttct gtacctgttc ggcacccccg gtcagaaacg
     1501 cttcgacttc atgtgggaaa tcctttccga gggcatgttg ggctttgttg tcatgataga
     1561 cagcacccgt ccggagacct tccgcgaggc ccgctccata ctggagacct tccgcgctta
     1621 tgcgcctaca ccgtatgttg tcactgcaaa taagcaagat agcgatgaat cttgggatct
     1681 agaggatatg cgcattgcat tgcgcttgtc ccccgaagtc aaattgcttc cctgcgtcgc
     1741 cttagatcgg gaagcggtga agaccgtcct gctggaatta ctttacagca tcctggctga
     1801 gatggatgag gagaaatcgt aacgcgaaac cgtgtcatcc atcaccactg atcaaggcat
     1861 tgtacattac gaagtattcg ggcgggggcg tcccgtcatc ttgctgcacg gttggctggg
     1921 ctcatggggc ttgtggcaag acaccatgac ctggctcggg cgatattatc gcacctatgc
     1981 gctagatttt tggggatttg gtgaatcggg caagaagcgc gattcttacg ccgtgcagga
     2041 ctttgttagc ctggtagacc agttcatgga acaattaggc attgcccagg ccccgctggt
     2101 agggcacagt atgggcggga cggtcagcct gtcggtggct atccagtatc cgcagcgcgt
     2161 gagcaatgtc gttgtcatcg gctctccaat caacggttca tcgctcaatt tcctgctgaa
     2221 attgttcggt taccggctgg tggcttatat cgtgcacaac aatttgtgga tattgcgctt
     2281 gggctttcgc ttactggccc ccctgtacac gcgggataag cgctggccgg agatgatgga
     2341 tcgagatctg tccaagacta ccctcgaatc atttctgatg agcattgcca ccctgcgccg
     2401 caccgatttg cgtccaagcc tggatcgaat ccgggtcccc gtgatgggta tgtacggcga
     2461 taaagatatc gtcgttgacc caggacaatg gaagccgttg ttggatggtg cacctcacac
     2521 tcgcgtggaa cgattcaagg atgcaggaca ctttatcatg ctggatgagc ccaaaaagtt
     2581 cgtcgagacc atacaggatt ttctgagttg cgagacatca agtccatgac aggagaagcg
     2641 aacattttga tgcataacta ttcgaacaag atggggcgga ttgtccttgt agcgatagag
     2701 gagatacttg gccgcaacgg catcaacgct accctaaacc tggccggcct ctcgcactac
     2761 atcaatagtt acccgcccaa taatttggac ctgcaattca atttcgacga tttggttgag
     2821 atccaggtcg ccttggaaga attatatggt cccttaggag gcaacggcat cgcgctgcgc
     2881 tccgggcgag cctcctttaa atacttcttg cgcgagtttg gcccgccgtt gggcatcacc
     2941 gatgtatctt tccgcttgct cccgctcaat gagaagctga gcactggcgc gcagctcttc
     3001 gctgagatat tcaaccgcta tagtgaccag cgcgtgcgcg tagaagatac gaaagagcgc
     3061 gtgtactggc atgtagagcg ttgtccgatc tgctgggggc gccaaaccga tcacccggtg
     3121 tgccacttca tcgtaggctt tctccaggag accctgtatt gggtcagtgc aggcaaatat
     3181 tttattgtgg aagagacaaa gtgtgtcgcc atgggcgacc caggctgtac catcgagatt
     3241 aataagcaac ccgtagatta gatgttaaag atcatcctgc agagccccca gtatatccaa
     3301 cccttcaacg agcgtgctcg cgatctgcgt gtccacaata agccactctg gctgtttcag
     3361 cgggatgttc tttctattta tacccatcga gaattggaac tgccccctcg ggctcctctg
     3421 cccaatacat ccgaagaatg cctggtatat cgcgataatc tgttcttcga tgccccgtac
     3481 atcgatgctt ttatcaagga agctagacgc cgcggacgcc cttgccaagc ggcgttttca
     3541 gcaagtgacc ttgctttccg ggagcatgct ctccccttgt ctgtctctta taccccgatg
     3601 gatgacttgt acctggcaga attatggtat tacccgcagg gtgtgcagct cgaatccgag
     3661 ccgctggtga tcgatctggg agcgagcgag gtggggtact atcatgtgcc catctatatg
     3721 gcttttgagc agggcgacct ggtatttcag gtaccgcgcc gtggtttgct tgccatcgac
     3781 tcttgggtgc atatattcat agcagatgtg gtctttgggg ttttttcgcg tggggcgcgt
     3841 tttgaggatc gcctgaagga cgatccgctc ttcaagctgc gtatcctggc gcgcgccatg
     3901 tacgagggga agcaagtgct tcagtcttcg gaattggtca aggtgggtcg gaactgcgtg
     3961 atcgatccgc acgcggttat ccatggtccg accacgattg gcgataatgt gaccatcaac
     4021 gccgggtcgg tggtggaaaa ctgcattatt ggtgacaatg ttaatatctc tcaaggttgc
     4081 cacttgatgc tatcggtggt gggggatggg gcgttcttgc ctttccgttc ctcgcttttt
     4141 atgacgacac tgatggacaa tagcatggta gcccagaata cctgcttgca gatgagcgtc
     4201 attgggcgga acacatttat cggggctggt tcgaccttca ccgatttcaa cctgctttcc
     4261 attcccctgc gtgccctgga tggcaatagc ctgctgaacc atgccaaccg tccagtattg
     4321 ggtggctgcg tggggcataa ctgccgtata ggctcgggga tgatcgttta tcccgcccga
     4381 accatcgagt cggacgtggt tttactcgct acgagcgagc gctatgtgat cacgcaggat
     4441 gtgcgctatg aagatagcga tcaccacagg ctgccttttg gtgaagtgca ccctcgcctt
     4501 tacccgcgcc aggctgaggc ggccagtgaa tcctggtaag taaaagaaca atatctcctc
     4561 cggggaattc gggatatgcc aatctgggat gtctcctgct tgaacccgaa ggtaaagaat
     4621 atattgggag gctcatatgg caaaaggccg gttgctcatt gtagaagacg atacagacat
     4681 ctccaccatg ttgcgtatct tctttaatgg cctgaattat gaggtggata ttgccgcgcg
     4741 cggcagcgat gcactggaaa agacccgcca aaacttgcct cacctcattg tgctggatat
     4801 catgcttcct gacatcgatg gcttcgaggt gtgccgtgtc ctgcgcacca atacgcgcac
     4861 cagccatgtg cctattatct ttttaactca gaaagacgag cgcagcgata aattgcaggg
     4921 attagagctg ggcgccgatg actatatcac caaaccattc gacattgagg agttaaagct
     4981 gcgtgtgcag cgtgcaatcc agcgcgccga tcagcagagc ctcaccgatc cccgctccgg
     5041 cctaccctcg gggaagttaa tcgaagagca gctccggcgt attatccggc ttcgaggttg
     5101 ggcaatgatg gacatccgca ttgaccactt tgaagccttc aaagaggtct atgggtttgt
     5161 tgccggcgac gatgttctgc gatttggcgc tatgcttatg ggagaggtgg tggatgagtt
     5221 tggctcacct ggcgacttca taggtcatgc cgggggcgat aatttcctgc ttatctgtgc
     5281 tgaagagaac gcctcagcta tccggcaggc gttaaaaacc cggtttgcca acgaggtgct
     5341 ctcgcattac aactttatgg atcgtgagca ggggtatatt gtcacttaca acgaagctgg
     5401 acaaccggtg cagtcgccca tgatgacact ctcgatcggt ctcgtctcgc cctcccaaca
     5461 gcagtttgct gatattcgcg agatcacgga gcttgctgcg gaggctagga ggagcgaagg
     5521 ttagatccta ttgcgattat ggctcaaatg agttcgactg gcttgggtat cgtgctgctc
     5581 gtcttgggat tgggcgtttt tgcgctcacc ctgtttttgg cgcgctccgt tccccgcaat
     5641 agagacgcct cacaggctgc ctccatgcct gccatgctgc ttgaaatttc ccggcattcc
     5701 gaggcgacgt ttttggtgca gagcggaggc cgccttgttc actacaattc agccgcttcc
     5761 gagctgttca atctggaagc agagccgaat ctgaacgagt tagggcgaag agcacggccc
     5821 agcgaggttt tttggggatt atgcgccgct gaagggcagg cgcgctttct cttagatggc
     5881 cgtgtcattg aggggatctc atatgccgtc ccatatggct ctggtcaggc tattctggtc
     5941 accttacgcc gcccaaaatt gttaacatct caccgagatg agaaggcctc agaagatggg
     6001 tcgagccatc agtatcaggg attggatatt cttgcagaag tcagccaggc aatgacctcc
     6061 agcctgaact tagaagcgac gcttcgctcc attatcgaga gcattgaata cgtgatttct
     6121 tgtgatcggt gtgaagtcaa tatctgggat gcagataatc gctacttggt tccacatagc
     6181 ctgaccggta cagtacaaat gcagcgcaag ctgcaaacca gtgaccggcg caatttgccc
     6241 aaccagggct acactggtta ccttgttttt caccggaagc cactatttat atcggatgtg
     6301 gataatttca tccaggctcg cccagccggg gacaaagcgg attttccgtt tcactcctat
     6361 gtcggtcttc ccctggaggc aggcggtgaa ttggttggga cgctggagct agcttctcta
     6421 gagaaagatg gttttaccga aagcgacctg gagttgctgc gcatgctctc ccgccaggca
     6481 gccctggcct tgcacaatgc gttatcctat gaagctcaag caaagcgcgc tggcgagcta
     6541 gctgggttgg cccagctagc tcaggttatc actgcgcagc aggaccccca ggatttatat
     6601 agtcgattag tcgaaagtat tgcaccgcta ttaaatgtta aaattcttgg cttttgggtg
     6661 atggatgaaa gccaacacgc gctggtgggg cagaccccct tcctgggaac acaaaacagc
     6721 gtggtggaat ggacgaaggt tatcctgcaa cccggcagcc aggctgaaaa gttatggctt
     6781 acccaagaga gtattgtttc tgatgatgcc tcctcggatg tgcgcatcca gcatttgggg
     6841 cttgaacact ttgccgtagc agcaggtgtt cgccagacgg tgctggtgcc actcaccagc
     6901 agcggtcaca tgcttggcta cctccaggca gctaacaagc gagatggcag tgacttcgat
     6961 caaagcgatc tacggctgct ggctattatc gccgggcaag ctgcgccgat tatagagaat
     7021 gctgggctgg tgcatgaatc cagtcgccgg gctcagcgtg ccgagatgtt aaggcgcatt
     7081 gccaatttga cagcctcctc ggcaaaattg gatgagatcc ttaagctctc tctacaagag
     7141 ctcgcccagt tgttaggggc tgatatggcg ctcatgcttc tttttgatga gggcaagggt
     7201 gagctgcgcc ttcaccgacc ttcgctattt ggtatctccc ccgatgtaac tctgcgatta
     7261 gggcgtttgc cagtggatga tcctgaattt cccaacactg ccacaggtag tttaaagtct
     7321 ttgttggtgg gggatgtgat ggatgagcca gatgtgatat cgacctatcg caacaccgcc
     7381 attaaccttc agggtcgctc gatacttgta acccccatgg tgttgcgcaa gcgaggtatt
     7441 ggggagttat tgctcattgg acgggatgtg gctagcttca atcctgccga attgcaggtg
     7501 gttgctgctg ctgccgatca gctggctgga gcgattgagc gcgccgactt gtatgcgcag
     7561 actgatgaaa gcttgcgccg ccgggtggat caactgaccg cagtcacccg tgtcagtcgg
     7621 gaactaagca gcaccttgca attggagcag ttgttgcagc gcgtcttcga agaggtgctg
     7681 cgcaccacac gtgctgactg tgggacgata gtcctctttg cgttgcatga agcggatttc
     7741 caaaacaacg tttcctcttc ttcaccacgc gtattgctac gcctcggtga atcgaagtca
     7801 gggctgcaca gcctggaacg catggtgcta aagaggggcg agacgctgat tttaaatgac
     7861 tttaatccgt taagggacgt ggtagatgcg ctagaagctg gaattgagct agctcagcct
     7921 ccccacgagg ggatccaatc ggcgatggtc atccctatct tctatcaaga tcaaattgcc
     7981 gggttgattc atttgcatgc ccgagcgcca tttcgttttg atgagtcggc gcgcgagatt
     8041 ggtgaaacac ttgctatcca ggcttccata gcgattggca acgcacaacg ctaccaggaa
     8101 caatatcacc gcagcgagct gctcgaccgc cgcatgggta ctattgcgaa actactggaa
     8161 acttcgcaag cctggcagtt ggaatcgccc ctggaaaaat ccttggagga aattgcttct
     8221 gcaatccagg gggcgactcc gttcgatgtg gtggtgatca gcatctacaa cccggatgat
     8281 caaaaactgc accgccttgc gaacgcaggc ttgtctttga cggatatgga ggagctccgt
     8341 gctcatccgc aatcttgggg taaattacag gatgttttga agccggaatt ccaaatcggg
     8401 cgctcgtatt tcattcccgt ggagcagatg ccggtaatgc caccagatgt gcactcgttg
     8461 attatccagc ctgttgaaga tgcagctaca gatgaggctg accatccggg ccgggtttgg
     8521 catcctgaag acgcattagt ggtccctttg cactctgctc aaggggagcc tttgggattg
     8581 atcagcgtag atttacctcg cgatcatttg cgccccgatc ttccgacaat cgagacgctt
     8641 gagatattcg ctagccacgc aaggttggtt attgaaagcc agcgcgagta ttcccgcctc
     8701 cagcagcaac tggataccat ccagcatgag cttcaactgt ctaaagaggt cgcggagacc
     8761 gctcaagcga acctgcccgt tttgctccaa aaggatattg accaatcctt ggctctgcgc
     8821 cgattaggcc aacacgcccg gcgcatccgt gctggcatcg atattgctga cctggctaat
     8881 agccaaagtg atcgctcgag cgtgcttcgt acattgggca acgagttcct cacccgtcta
     8941 aatttggatg ttgtcttggt agttgagcct ggattaggcg gaccacactt gctggaagct
     9001 ttgggtgaat tgcccagcga gatcaatttg gaagcactga tcgggcagcg caacccgcta
     9061 tatcattgcc tgcgcaccca ggatcaattg ctggttgatc gcctggagag caagatgaaa
     9121 tgggcagatg cacccttatt acaggcgctc gacgtgcaga gttttatttg cttcccgatc
     9181 ttgagcggcg atcggctgga cgcggctgtc ttgggagtaa gtcgctctcc aatggatcca
     9241 tttaccgaag acgatgaaca gatcttcacc ttgctctccc gccaggtagg gataacaata
     9301 caaaatctac aactgttagg tgagaccagc cagcgcctgg cagaagtcaa cctgctgctg
     9361 gactttaatc gccgcttggg ggggttgaat ccgactggca tcgctcacac cctgggacaa
     9421 agcattttgc aactggtacc caacagccag gctgttctgg tcgcactgtg ggatactcag
     9481 cggcgtgccc ttgtccctca agatgcaatt ggatacgcag atgccacgcg gatgatggaa
     9541 attatataca accctggcga agccttgcct ggtcaggttt ttgagagtgg tcaagctgta
     9601 attttggatg aggtggattt tgccaagcat tacagcctta cgtccgaaaa ccttctgcgc
     9661 tttcgtaatg caaccggtgg gaagttgccg gtatctacca tagtcgcgcc tatcctgccc
     9721 gccgaagggg cgcaggcttt gggcatcgtt gtggtggata atttccagat gtcagccgct
     9781 tttactcctg aaatattggc gctggtaacc tcgctagctc agcaggcagc tttgaattta
     9841 gagaatactc gcctggtgca ggctgctgaa gagcgcgcca gccaattgca ggccttgacc
     9901 ggtgtagccg ctacaattac atctaatctg caacctgata acctgattgc aatgcttttg
     9961 agcttgctgg gtgcaatcct gccatatgat acgggcaccc tatggcttaa gcaggatgaa
    10021 aacgaaatga tcgtgcgctc tgcgcacggt ttctcagata atgagcagcg tattggattg
    10081 actgttgcat tacaggatag tattttactc agcgatatga tcgccagcgg gcagccggtg
    10141 gcggtgggtg acattcgcca tgatgcgcgc ttttcctcct taatagaacc gcaatatatt
    10201 tcctggctgg gtgtgcccct gatcaccagc ggcgaagtga ttggcgtaat cgccctggag
    10261 aagtccgagt ccaattatta cactcaagag caccgccaaa ttgcgacgac atttgccagc
    10321 caggccgcag tagcgctgga aaatgcccgt ctctaccacg agagtctcaa ccgtaccctg
    10381 gagctggatc aacgctcgca gcgactggag atgctcaatc gcttgtctgc agccttgatt
    10441 gcaaccctgg agccggagca catcttgcag gtggcaaccc aagagttagg gcgagcgatt
    10501 caatgttctg gtgtgtctgc ggtattgttc gatgcggatg gtgtagggaa attgcacgcc
    10561 gaatctcccc aggtggagga cgtattgccg tgcgccttac cagatgtccc gttattcgag
    10621 cgcctgcgcc agacacaggg tgtgttcaat gccggggata tcggcgtgga gagcgaattg
    10681 gcgccattgt ccgaatttct tgaggcccgg caggcgcgtt cactcatggc attgaccctg
    10741 gccacgggcg aggatactca gggtatcttg ctggcatacg ccgatcggga atcccgcttt
    10801 accccagatg aggttgggct ggcccgcacc attgccaacc aggtttcgat agccattcaa
    10861 aatgctcgtc tgtatgccga gacccgcagc ttgagccaag atctggagca gcgtgtcata
    10921 gagcgcactg cccagctgga gaaagctcaa cagcgtgccg agaccttgct gcgcatcaac
    10981 atggaattga gtaccagtct agatttagag caggtgctca atcaatccct acacctggtc
    11041 aaccagattg tggacgcagg gcaggtgtct gtgttgatca cccgctcggg cgagaagaag
    11101 ttttaccgct tggcttcccg gggctatgct ggctcgcccg acagcgatgg gcagaccacc
    11161 ctcatcaatg ttgatgaggg tctagctggt tgggtgatca aagctcgcca aacagtattg
    11221 gttgacgatg tgttgcaaga tccgcgttgg cttaagctga cggatgcacc cgagcctcat
    11281 caccgtagtg ccttggctgt tccgctgctg aggggagcgg agtgtgtggg ggtcttgctg
    11341 ttgtaccacc aagatgtagg acatttctcc gacgaccacc tggatttgat ccaggctgtc
    11401 gccaaccagg tagcggtggc ggttaataat gccgagttgt atcgcttgat ccgcgaccag
    11461 gcagaagatt tggggcaaat gttccgtcgc cagcaagtgg aggctagccg tgcgcgtgcg
    11521 atcctggaag ctgtagccga cggcgttttg gttacagatg ccaaacgaaa tatcaccctg
    11581 ttcaatgcgc ctgcagagga gatccttggg cttgatcgat cacaggtgat tggtcattcg
    11641 ctcgaccact tcaccggctt gttcgggaga gccgctcagc gctggaatga gactattcgg
    11701 acctggacac aggatcccag cacctatgag cagggtgaac tctacgccga gcagatcgat
    11761 ttggatgacg ggcgggtggt gtcggtgcac ctctcgccgg ttatcatgga ggataacttc
    11821 ttgggtacag tctccacgtt cagagacatc acccaccagg tgcagctaga tcggctaaag
    11881 agcgagttcg tagccacagt gagtcatgaa ttgcgcaccc ccatgacttc tatcaaaggc
    11941 tatgtcgatg tcttgctgat gggcgcagct gggtcactca ccgagcagca agttgagttc
    12001 ttagatattg tgcgcgataa cacggaacgc ctgaccgtcc tggtcaatga tcttttggat
    12061 atatcccgta tcgaatccgg tcaggtaact ctctccatgc tgccgcttaa cctggatgag
    12121 atcattacac aaggggcagc tgccttacgg acccgcgctg agagcgatca aaagccgatg
    12181 gatattcagt tcgcccccgc ttcggaattg ccgcttgcta tgggtgaccc agaacgcgtg
    12241 cggctgatcc tggataacct gctggagaat gcttactatt acactgcgcc aaagggtcgc
    12301 ataaccgttc aagttcatgc aactggggat gaaattcaaa tagatgtaca agatacaggc
    12361 attggcatcc ctgaagaagc gcaagctcga gtcttcgagc gctttttccg cggtgagcac
    12421 ccctttgtcc tgtccacctc agggactggt ttagggctct ccatcgtcca gcacctggtt
    12481 gagatgcaca atgggcgcat ctgggtcgaa agcagcggcg ttcctgggga aggaagtaca
    12541 ttttcattca cattgccagc gtttcagcat gaccaagcag ggagtcaaaa gttccacgat
    12601 gagcaggtca gacagttagc aaaggagtaa cctggtagcg aatctgatga aatttctctg
    12661 ctctcttatc cagtatcgtt tggcatggat cataaaaaca tggcgcgcat attaattgcc
    12721 gaagacgaaa aagatatccg agaattaatc actttcacgc tgagtttcgc tgggcatcag
    12781 gttacggcga cgaccaacgg ggaagaggcc tacttgacgg cacaggaaat gcttccagac
    12841 ctgatacttc tggatgtacg tatgccgcgc atgactgggt ttgaagcttg cgaaaagata
    12901 aaggccaatg agaagacacg ccaaattccg gttgtgtttc tctcggcgaa aggtcaagag
    12961 gtagagattc agaccgggtt gaacctgggc gcggttgagt acatcgtcaa gccctttgcc
    13021 cccgaacaat tggctgagcg tgtgggagct gttcttgcga attttggtaa tggttgaatg
    13081 atctgaagat caggtgctac tgactcatga gtgctgtcat cctgcaggac gaaattgtgc
    13141 attacgaagt gctaggccgc ggacgcccgg tcttcttcct gcacgattgg atcggctcct
    13201 ggcgctactg gatcccaagc atgcaggcga cttccttatc atttcgtgcc tatgcactgg
    13261 atttgtgggg tttcggagat acaagcaaga atcccagcta ttattccatt gaacaacagg
    13321 tcggtttatt ggaagaattt gtcaacaacc tgggcattgg caagattgcc ctggtgggtc
    13381 atgggttggg ggcagtcatc gccatgctgt atgccagccg ccaccccgaa ggggtagacc
    13441 gtgtcatgac catcggcatg ccattcggtg aggatagaat taactcgcgc ctgcgcctaa
    13501 actccccgag cgagctggcc agctggcttt tggggcgctc accggctgct gaggcagcct
    13561 atgtggatgc tcccaaggcg gaccataaag ccattctgct ttctctgacc agcctgcaag
    13621 gggtggaatt ggcctgcctg ccccaccagc ttgccactgc ttgcctgcta gttcacggcg
    13681 ggaatgatcc cctcatcgaa acgcccaact ctaatcacgt agaagagctg cctgaagaat
    13741 ctcaatttat catattcgag aagtcggggc acttccccat gctggacgag ccaggtcaat
    13801 ttaaccgcct tctggtggat ttcttaagcc ttgaatcggg gcagagtccc cgacaactgc
    13861 agctcaagga agagtggaaa agaagggtaa ggtaatctgc tcgtagtggt ggacgccagt
    13921 ccaccagccc aatcagtcca acccgctgag acacacagag cgcagacgaa gattttccct
    13981 acacgaagtg atcaacacgt aaattcctta agtctgactc caaaaattag agaaccatta
    14041 gttaataatc caaaacatct ctaaattatc gcaatatgcg ctataatatt tttgttgtag
    14101 ccacggtctt tggaaataat tgacaagtta gcgattgtca atgggggcat aaaccgggac
    14161 ctcaacgatg catctgcaaa gtcccagatt aatttagcaa tagttaatat ggggtatacc
    14221 cacggcaata gcggatgtcg tatcactgct cattacatca attcctggtt catccaacct
    14281 tgccaaccaa atgtgcgcat cggaaactgc gcccaatcat acccaaagga gagcaagatg
    14341 acgagtaaag cagacgttat acgaaggtgg cataaggatt catgggccaa tccgccctca
    14401 agcatcgggg aagcggctac gaagtatttc tctgacgact atcaaggtct cgacaaagac
    14461 ggaaacgtta tgtcggacaa agcagcatat atcggcatga gccaactttt actgactgct
    14521 tttatcggtt ttaagggggt ggtccacgat atttccgaag agggtgacag cgtcatattg
    14581 acctttcatt ttgaagggac acatacgggc gatttggacc tgtcgatgat gggcctgggc
    14641 gtcatcccgg ccagcggcaa aaggatcgta acgccggaat ccaaaacaag gtttaaggtt
    14701 gaaggcaatc aaatcgtagg ctcccaaccg atttccggag gatttgagtc gttactggca
    14761 ggtttagggg tgaagccgcc gccaggataa tctaacactt cactgccggt cactgcaaaa
    14821 aatagtacgc gagatgtggc cacgagtgct gagcgcgtgc cggcaagcca ttacgatggc
    14881 ttgccgtcgt ttaagcggtg aaagattaat atttgtataa tatatgaaca atatattgac
    14941 aaaacgtagt taatctgata tattatataa aatattacat gcttaaggag agtacaatgt
    15001 caaagtcaac caaagctatc gcactaatgc tagttctagc ttttacctta tcggtcttcg
    15061 tctcgggtgc attggctgga ccaaaaggac cgacgattgt cgatgtggcc attgcggtca
    15121 acagtgatgg ccccttcgca ggccagtttg acactctgat cgccgccgta ctggctgctg
    15181 atccagcagt gctgaatact ctatccggta atggccagtt tacagtattt gcaccaaccg
    15241 atgccgcttt tgcagcgctc gagctcaccc ctgataatat cggcacgcta cctcaggctt
    15301 ttctgaccga tgttttactt tatcacgtgg ctcacggttc gcgctatgcg gaggatgtgg
    15361 ttgattcgaa gcgcatccgt acactgaata agagcttctt gcttcaagat ggtggagtgc
    15421 tcaccgataa tctgggtcga gaagccacta ttattgtgac tgatgtgccc gctgccaatg
    15481 gtgtcatcca tgccattgac gccgtcgttt taccatacgc cccgtaaaca actactctta
    15541 tttttaatgt gtcaagaccg gctatcaaag ccggtctttc actatgggga attgggatca
    15601 ttgaacttca tggatgcagg aatatttcgg atttcttctt tatcctcatg ataggtctgc
    15661 ttcaacattt actgatcaat gcagacacca ggaatcactt gctcccattc cttgatctct
    15721 tggatatcac caatcctatc aatcgctcta ggaagactca ccaatcatat cccgtaaagt
    15781 gtatatttct gctgaacaat aacacgacaa catcgcccca ctccttgaca acggctaacg
    15841 ctcgcaatat actatctgta caactaaatc cttgcacggg gagcctggtg atgccgggct
    15901 gagaggaggg ccgacagccc tcgacccgcg aacctgatcc ggataatgcc ggcggaggga
    15961 acgcaaatat cggaaaaatc acccctcctg ccgagccaac aggaggggtt gcttttgacc
    16021 acacccacgc gtgccgtcat ctttgctaac ggggtgctga accaccctga aacagccttg
    16081 gctgccctgc gccccggcga tctaataatt gccgccgatg ggggcgcgcg cctgtgccgt
    16141 cagttgggct tagtgcctgc catcttgatt ggcgacttcg actcgatcga cgaggctgac
    16201 ctggcgtatt ttgacgccaa tgggacacag atcatccgcc accctgcgcg caaggattat
    16261 acagatctcg agcttgcttt gcgtcatgtc aaatccctgg gcatccaaga cgtcctggta
    16321 gtcgcggcgt tgggggcgcg ttgggatcag acgctggcta atttgttgct cccggcggct
    16381 gtcgacctgg gtgatctaac cattcgcttg atggacgggc cgcaagaggt tgtgctatta
    16441 cgcggcggtg atgagatcga tatccatgga aagcctggcg acaccgtatc tatcatccca
    16501 gtggggggtg atgtgcatgg ggtagtaacc gacggtttag aatatccctt gttcaatgaa
    16561 tccttgttct ttggcgccac gcgcggcctg agcaacgtgc tgctggttgg gcgtgctacc
    16621 atccatctcg agcaggggtt gttgttatgt gtagtgatcc atcaggatgg atgatgaaat
    16681 tcgctgcttt ttcgcactat cgtcattcat cctggggacg aaacggaggt aagaatgttt
    16741 aaaaatatct cccttattag atcaaattgt cgttatgcgc cccccgaagg gcggcgtggt
    16801 aatctccttc tcgatttttt tgcgtggccc cagaagtgct ggccgctttc tattctcttg
    16861 ctcttcttga tcttgggcct gtctgcctgt gctcctgcga gcctggcgcc aacttcgact
    16921 ggtgagcttc cgacggtcac cccaagcggc ccacgaacct taaccgtgat gacccatgat
    16981 tcctttgctg tctccgacga ggtgatcctg gcttttgaga gcgcctacag cgtggcggta
    17041 aaaatcctcc cctccggtga tactggcacc gcgctgaaca aagccatcct ctcgaagggc
    17101 aaccctatcg cggatgtttt ctacggtgtg gataatacct ttctcagccg cgccctggac
    17161 gaagatatct ttgaacccta tgatgccccc gggctggcag atgtccccga ggctttcctg
    17221 ctcgacccgg gtaaacgcgc tcttccagtg gattacggtg atgtgtgtcc aaactacgat
    17281 aaaacctact tctctgagaa gggattggcg ccacccagga cgctcgaaga cctggctaaa
    17341 gcggagtata agggtttgct ggttgtggag aatccggcta cttcatcgcc tggactggcc
    17401 ttcctgctgg ctaccattgg gcgctttggg gcagatggct acctggatta ctggaaatcc
    17461 ctggtggaga atgatgtact ggtggtaaac gattgggaaa gcgcctacta cactgagttc
    17521 agtgctcatg gcgggacgcg tcccatcgtc atttcttatg gctccagccc cccagccgag
    17581 gtgatatttg cggagtcgcc catcgaagag tcgcctaccg cggtcatcgc tgcagggagg
    17641 agttgtttcc gtcaaatcga attcgcgggc atcttgaaag ggtcacagaa tcgtgatctg
    17701 gccgagttgt ggatggatta catgctttcc aagacgtttc aggaggatat gcccttgcag
    17761 atgtttgtct tccctgtcag ccgtcaggcg gtgctgcccg atgagtttgc caagtacacc
    17821 tccatccccg ctgacacggc ccaggtaaac ccagatgaga tcgcagcaaa tcgcgagcag
    17881 tggttgaata gctggacgga ggcggtgctc agataatggc gttggtcaca tgaagtttcg
    17941 tactgtatat tttctctggt tggcacccat agccttcctg gcaatatttt ttttctaccc
    18001 tttgggcagt attctggcgg tcagtttcgg gcgtgccctt ggagaggata tgcttgaact
    18061 tccgcgagcg ctctgatcac cttccattcg ccgtgtggtg gcatttacct ttggacaggc
    18121 aaccctctcc acgctgctga ccttgctgat ggggttaccc ggagcgtatc tgatcgcccg
    18181 ctatgagttt cgcggtaaat cgctctttca ggcgctcagt ggtatcccct ttgtgctgcc
    18241 cacgctggtg gtcgccgctg cctttaatgc attgctcggg ccgcgcggct ggatcaacct
    18301 ggcgctcatg gattggttta acctgaccca gccacccatc cagttcctca acacctttac
    18361 agctatcatc gtcgcgcacg ttttttataa tttaaccatt gtgctgcgca ttgtgggtga
    18421 tttttggtcg cacctcgatc cgcgcctggt gcaggcctcc cagatgctcg gagccagtcg
    18481 actgcagacg ctgttacgca tcaccctgcc gttgctggct ccggctatcg cagctgcggc
    18541 gttgctggta ttcatttttg atttcacgtc ttttggagtg atcctgattt taggaggccc
    18601 gcgctttgcc acgctggagg tggagatcta ctatcaggcg atcagcttgt tcaacctgcc
    18661 tatggctgct accctggcgt tgctccaact ggtttgcacc ctggcgatga ccactatcta
    18721 cacccgcctg gcagctgggt tagcacgccc attgggcctg cgcccgcggc gctttaccca
    18781 gcgccgtctg gtgacctggc gtagccgctt ggtagctgcc atgctggttg gcttgatgtc
    18841 tgtcttcctg atcactcccc tggctgctct tgctactcgt tcattcacgc gagtggacgc
    18901 tgggagtcag gtaggcggaa gcgcgggcga cataacattg gagtggacct tagattacta
    18961 ccgcgagctc tctatcaacc ggcgcgggtc gctctttttt gccccaccct caacggcgat
    19021 cgctgtgtct ttgggatatg ccgcggccac ggttgtcctg gcgttgctcc tcgggttgcc
    19081 agcctcttgg gcgctcgccc gctatcagtc ttcagcgcta agtcgcctgc tcgatccgat
    19141 cttgatgctt ccgctgggca cttcggcggt cactttggga ttgggtttca tcgtcgccct
    19201 ggaccaaccc ccactcgacc tgcgcgcttc gccggtgctg gtgcctctgg cgcataccat
    19261 ggtggctttt cccttcgttg tgcgcagcct gacgccttcc ttgcgcagta ttcgcccgcg
    19321 attgcgccag gcagcttcag tattgggggc ttcccctagc caggttgtgc gtaccattga
    19381 cctgcccatg gtggggcggg cattgctggt ggcggcgacg tttgccttca cgatctccat
    19441 cggtgagttt ggcgcgactg cgctgatcgc ccggcctgaa tatccaacca tcccaattgc
    19501 catttaccgc tatctgggca tgccaggtgc gctcaattat ggtcaggcgc tggcgctgag
    19561 cacgatcttg atgggcgtca ccgcggcggg catgctggcg attgaaagat tccgcatcgc
    19621 ggaagttggg gagttctagt attgaataca aagttagggc ttggcgtaca agggattcac
    19681 aaaacgtttg gagaaatccg tgccgtgtca ggcgtctcct ttgatgtaga ccctggcgaa
    19741 gtcatggcat tactcggtcc cagcggctgc gggaaatcca ccgtattagc ggtcatcgcc
    19801 ggtttggagc aagccgatgc cggtgggata agctgggacg gcaaatcctt gcgggaggtg
    19861 ccgccgcacg agcgcggttt tggtctgatg ttccaagatt atgctttgtt cccacacatg
    19921 aacgtgtttg gtaacattgt ctatggcttg cggatgagag gctggcctga agatcgcctg
    19981 cgaaaacggg tgggtgacat gctggagttg gtgggtttac caggtttcga taagcgtgat
    20041 gtgaacaccc tttcgggtgg cgagcaacag cgcgtggcgc tggcccgttc gctcgctcct
    20101 gagccgcgcc ttcttatgct ggatgagccg cttggctcgc tggatcgcaa tctgcgcgag
    20161 cggttggtct cagatttgcg ccaaatattg aagagaatgc atcaaactgc catctacgtt
    20221 acccacgatc aggaagaagc ttttgctttg gcagatcgcg tggctgtgat gaacgcgggg
    20281 cgcctggagc aacttgatgc cccgcagatg atttatgccc gtccagcttc gcccttcgtg
    20341 gcacgctttt tgggaatgac caacctgctg gatggcgtga tccgccccac gttggatggg
    20401 atcgtcgtgg agacggcggt tggcgcgttc cccgtcgttg gccaatttca aggcagggtc
    20461 acattgctcc tgcgccccga cgctgtgcgt ccggatgggg tggggagtat gcatctccag
    20521 ggcaggttgc tcggttgttc cttccgcggt agctcctgcc aggcgactat cgaagtggat
    20581 ggcgttccat taaaatttga ttttctgact agcacgccct tgccgcctgc cggcgaatta
    20641 gtgaccctgg gatttgagcc tggggatgca ctgcaagtgt tctcgcaatg aagccaagcg
    20701 catacgctgg tttgagatag aatagggcat cgcagctgaa ctaatcctac aactacgcaa
    20761 gcgctcttct caatatgtct tcgattacgg taattaagct cgatatcaat ggggtggaga
    20821 cctggcgtta tccaggtcag ttactgcaca aacaagacac gcaaattgtc ctggaggcgt
    20881 attttgaccg cgacgacatg atctttcatg gaatgcctct tcacagaggt gatcgcttcc
    20941 tcgaattgta ttttacagac cgctggtata acatttacga aatccacagc cgcgcagatg
    21001 atcatctgcg cggctggtac tgtaatgtgt gtacccccgc ccagctagat ggggacaggt
    21061 tgtttttcaa agatctggct ctcgatttgc tggtcttccc cgatgggaac cagattgttc
    21121 ttgatgagga tgagtttgcc cgccttgagc tttctccaga cgggcacagg ctggctttgg
    21181 ctgctttgga tgaactgcga gcgggtttca aacagccagc atggctcgaa gcgttaggaa
    21241 accctggcta tctaaccatt ccaagagcaa atctcccagg taaaaataga ataggagaat
    21301 gaaggcgcag cacgcgcaga gaagtaccaa gatcacgatg agaatgatca gcgtggtatt
    21361 tcttttcttg ggttcttgca tgggggtgat gggtgtctcg aattcttcag acattgctcc
    21421 tccttgtagg tttcagtgag attgtgaaag ctcaggctgt tgatatggag ttggttctac
    21481 gccacatgcg ccagaccacc caggcggtga acaagatcaa aacgctaccg atcaggatgg
    21541 ggattactat ggagaggatc gagagcacgg tcgagatgaa gtcttccagc atgctgacag
    21601 gcacgttgcc tgcgccacct gtagttatgg tgactgctgg tcgaactgcc aaggatttaa
    21661 cggcgtgcac tccgcctgcc accagtaccc ctgccgccat ggagaggatg gggtttacat
    21721 cggtaatcac tttggcgctg gcggcgaaag cgagcgctcc agcaaccggg cgcacaatgg
    21781 tctggatggc gtcattaatg tgattcactg ctgggatctt atcggcgaaa aactcgacca
    21841 gggacaggaa taccaaaagg ccaatgatcc accagctttc cagggcgttc cagggagaac
    21901 tgagctcgat caggtctgtg aaacgtgcca gcagggcaat caccagcagc gggatatagg
    21961 cgttcaatcc tgcgctggct gatagcccaa atgcagagaa aaccccagtc atgacctcca
    22021 tagcaattgc ctaattttac cctctatttg ccaattgcca aatatcaggt aaggcaatcg
    22081 catttgggcg atttcccacc agtgaggaga gaaaatgggg tgttttggtg ggggtatgca
    22141 cccaccaaaa caccccaaac actctttttg cgggactgtg catgcccctc agggcatatg
    22201 caattgccct gaatatcagg cgatatccaa aaaagtttcg taataaaagg aatatgacgg
    22261 atttaattaa ctcagcccag atgaaatcca tcccaggtac cggtgagcag gtagcggatg
    22321 atatccagta atgcaatctg cgctaatccc aggccgaacc ccgcggtcaa tggcagcatc
    22381 atcttcgcta gctttgtata agtgttttcc aagcgaaaga tcatcatcga aaccattgaa
    22441 tataccagcg ttaattcaac cagtacggcg atggcgctga gaagggcgag cgggtagagg
    22501 atcaagggat tttgagtgag cacaaccagg tctagaatag cggctagcag caccagccaa
    22561 gcgagttgtc taaccccgct caacgcgggt tgaggctgcc agtatcgcca ggttgtctgg
    22621 ttgaaagccg ggaagagcag tgcagccatc acaaccccca tgctcgttcc ggtgaacaac
    22681 ctcagccaat tctgcggtgt gtatagtgtg ggagccccgg gaatgaggga gagaaatgag
    22741 ttgccgccgt ctaagaaaaa agcgcttgcc agcaaaccca ggacaaccag gacacgaatt
    22801 gggggtaagc ctgctttgcg gcggctgaag gcgctctgat aaaccaggcc gagtaaggcg
    22861 cccaggtgca tgccggtgca gcgcgcgcac aatggtagcg tgcggtctcc tagatggaag
    22921 gagcgcaggt ctatgcggtg gcacaccgca taaccgattg cgtctgcttt gcccagtaag
    22981 ccagggggag tgttgaaaag ccagcctagg aataggaacc ctacggcaac gaaaaccagc
    23041 aatttcaaag tctggctatt tgtgtgcagt ttttcccgtg catcagccat acgagttgaa
    23101 ttatagagtg tttcaagaaa aacgcaagtt ttgcggtatg attacccagg gttttcaaaa
    23161 ggatgggatt tggggtcctc cttgggcttt cacaaggttg aaagccctgt atgattacct
    23221 tttgagtttt tttctggaga atggagatta tgatgaacaa gcgatggact ctggttggtt
    23281 tgatggttgt ggctctgagc gcatgttcaa cctttgccgc gcctgcgccc acgcccacta
    23341 cagaggcgac cagcaccccg agtctagcac cgagcgaaac acccacagta acaccaactg
    23401 tacaacccac ccctactgcc acagaaacgc cttcgccacc caccgccacc ccactcccgc
    23461 aagacgttgg cccagcgaat ttcccgcaag gtgttaatcc tctcaccggg ctaactcttg
    23521 ctgatcctgc cttgctggag cgccgtccgc tggcggtgaa gatacagatg ttccctcgcg
    23581 gccagcgccc accctggggt ctctcgctgg ctgatattgt ctacgattat tatcaaaacg
    23641 atggcatgac ccgcctgcat gccctctttt atggaaacga cgctgaaatt gttggcccga
    23701 ttcgctctgc gcgcttgttg gataaccgcc tggtgcgcat gtatcggtcc atctttgcct
    23761 tcggtggcgc cgaccagcgc atcttgaatg tcttgctgaa ctcggaatac tacgaccggt
    23821 taatcgtgca aggtagctcg tctccgatgt ggcgggaaga tcccaatggg gcaaattatt
    23881 tgcttaccgg catccaacaa atggctgagt acatcaatag caagggtatc tcgaacagcc
    23941 gccagaacct ggatggcatg cgctttgaac tccaacctcc cgcgggaggt ggatcggcga
    24001 gccaggtatt cgtccgctat tcgatcagcg cttacaatcg ttgggattat gacccaacga
    24061 ctgggtctta tctgcgttat gtggatacgc aggaggatac gggacaaggc gagagttatg
    24121 agcagctggt tgaccggttg acggagcagc caatatctgc ccagaatgtg gtggtagttt
    24181 tcgccgttca cgatgattac cttaggaatg ggcgatacaa tgtcgtggat atcttgctcg
    24241 atgtcagcgg tccagccttt atcttccggg atggacaggc atacgaggtt cgctggtacc
    24301 gccctcagct ggaatcggtt ctgtctctcc tagatctgaa tggcaaccca tttccattta
    24361 aacaaggcaa tacctggttc caggtgatcg gccaatcttc tcgctggggc aacgaaagcg
    24421 gagtatggcg cttcgaaagc cgcatcccat agaagtcatt ccagcctgcc tcaagttagc
    24481 ggtatgaaaa cgggtctttc tagcattgcc gtgctctggg cgttgatgat ctcaggctgt
    24541 gccgcggtcg cattagaccc gcaggtttca gtgtcttcta caccgccagc tgaagtcagc
    24601 gagattgcca ctgaagaacc aagcgctgct cttgaagtga tcgccacgat ccctatcgcc
    24661 actgagacgg ctacccccag cgtgacgctc gagccaccaa ccacggcaac ctcatttgtg
    24721 tatgcctacg ggccgagtga ttttcctgag ccgattaacc cactcaccgg tctgccggtt
    24781 gacccgccgc aactgttgga gcggcgcccg attgtcatca aggtgaccaa ttttccacgc
    24841 agcgtacgac cacagtgggg gctatcacaa gcggatcatg tctacgaata ttacattggc
    24901 gatagcatgt cgcgttttgt gggggttttc tatggaaaag atgccgaacg ggtagggcca
    24961 atccgctcgg cgcgcctttt cgacgcgcat gtcatgcgca tgtatcatgg catttttatc
    25021 tttggatggg c
//
LOCUS       DBNGSX010000024        17945 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1317335, whole genome shotgun sequence.
ACCESSION   DBNGSX010000024 DBNGSX010000000
VERSION     DBNGSX010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 17945)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17945)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17945
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1317335"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            532..1416
                     /locus_tag="AC16CB_00685"
     CDS             532..1416
                     /locus_tag="AC16CB_00685"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3341007.1"
                     /translation="MDIGFVGLGVMGEGIVPRLLAAGHSVTGWNRTQGKAEPLIKLGM
                     HWADSPREVAKQSEIVFSIVTDAAAVRAVALGTDGIIAGLREDGIYLDMSTIAPDASR
                     AIAAEFESAGLTMLDAPISGSPITLAQGKASIMVGGNKDAFERVHPVLLDIGAKAMYI
                     GGNGLAVQMKLAINLVLMVEVIAFGEGIALAEKGGVKREVAVEAMLNSVAASPVLGYR
                     GPFILEGNMPEVPLADVTLQQKDMILVLDLGRKLGSPVPLAAAANEMMNACRGLGIDH
                     RDFVTAHEVYRVLGGMLK"
     gene            1413..1829
                     /locus_tag="AC16CB_00690"
     CDS             1413..1829
                     /locus_tag="AC16CB_00690"
                     /inference="COORDINATES: protein motif:HMM:NF012417.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3341008.1"
                     /translation="MKPYRTNLRDVPYVEGLSPRDGWINMQVQFLIDEKTANSDKLVL
                     GWTVLPPGARHDRHRHFQADEFLLVLKGHGEIYTDEGNEPAGEGDVVYTTRGHWHGFN
                     NTSHEEVVLVWGWSGAGSLESAGYEVSHDHSHSDQA"
     gene            1833..2537
                     /locus_tag="AC16CB_00695"
     CDS             1833..2537
                     /locus_tag="AC16CB_00695"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: protein motif:HMM:NF013706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008684 - 2-oxopent-4-enoate hydratase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HIR3341009.1"
                     /translation="MNVAGYDSRILRGMEKLLKLRQGRLDAGEKSIGWKVGFGAPASL
                     EALRLDAPLVGFLTDKVLLDSGSALSIAGWTKPAAEPEIAVYLNRDLPGVSDRETIQA
                     AISAIGPAIEIADVHFPPNDVEAILAGNIYNRHVILGKSDNSRAGCTLDGLVGRIYRN
                     GQEIATVTDLQVLTGNFIGIVQHVANLLASLGQMLRSGDVIITGSIVPPLWIERDEEI
                     RFDLDPIDKLTIRLEN"
     gene            2729..3427
                     /locus_tag="AC16CB_00700"
     CDS             2729..3427
                     /locus_tag="AC16CB_00700"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR3341010.1"
                     /translation="MYQPIQSERLYERIVDQIERRILAGDLKIGDQLPAERELAEQFA
                     VSRTAVREAIKALREKGLVEIRLGRGTFVTSGAAGAVRRSLGLLLKTENGFVNLEEVR
                     EILEPEIAALAATRISEEYITAMTEAVETMDTALDNVEIFVEADLDFHLALAEATQNP
                     IIPSLMDSIIDLLREQRKRTGNVEGGLARGQYHHKKILEAVIQRDPQAARKAMQDHLQ
                     QVREDTYASAKNTD"
     gene            3449..4327
                     /locus_tag="AC16CB_00705"
     CDS             3449..4327
                     /locus_tag="AC16CB_00705"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3341011.1"
                     /translation="MANLGFIGLGVMGGRMVKRLLDAGHSVTGYNRTRSKAQWLLEAG
                     MTWGNTPRAVAKSADIILSMVTNTEALHSIAEGPDGLLAGLSAGKIYVDMSTVSPAFS
                     RELAKEVAIKGASMLDAPVSGSVVTLEEGRLSVMVGGDAETFEKAKPILQDIGPKATH
                     VGANGLAVTMKIATNLSLAVQMLAFSEGVLLAEKSGIKREIAVEVLVNSVIASPMVKY
                     RGPFVLEMPDEAWFDCYMMQKDMNLALELGRELSIPLPTTAVTNEFLSAARGMGLEKQ
                     DFAIMFDVLAQMSGVK"
     gene            4327..5331
                     /locus_tag="AC16CB_00710"
     CDS             4327..5331
                     /locus_tag="AC16CB_00710"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR3341012.1"
                     /translation="MANETQVTANPKPETLDVEQWLHAYEQMAKIREFEEKANELYMK
                     ALMPGLTHLYSGQEAVAVGVCEALRRDDYITSTHRGHGHCIAKGASVDLMFAELLGKE
                     AGYCRGKGGSMHIADPESGNLGANAIVGGSAGIATGAALSAKMRGTDQVAVCFFGEGA
                     LGQGLLYEVMNMASLWKLPIIYVCENNLYNEYTHYSEALAGEVTERARAFGIHTESIY
                     GQDVQLVYETMRKLVERARRGEGPAFLECLTYRYYGHHVGDINRTYYRSKEEEQEWRE
                     VHDPLKLLEEKLVASKLTDQDVLERIYADAKNEVEAGVQFAIDAPYPDQSQVTEDVYA
                     "
     gene            5347..6354
                     /locus_tag="AC16CB_00715"
     CDS             5347..6354
                     /locus_tag="AC16CB_00715"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR3341013.1"
                     /translation="MGGSTINMAEREITFAEAIREALAEEMRRDQNVFIIGEDVAEAG
                     TPFKVLSGLVEEFGKNRVFDSPISEPGFTGMGVGAAMTGMRPVVDIMFGDFLTLAMDQ
                     IVNQAAKVHYMSGGKLKVPIVFRTTMGATRRSAAQHSQSLQAWVSHVPGLKVTMPSTP
                     YDAKGLLKTAIRDENPVVFIEDKMMYKIKGPVPAEDYTIPFGVADIKLQGDDITLVAT
                     SSMVQVALGAAEKLKDIGISAEVIDPRTTTPLDKQTLIESAKKTSRAIVIDEGYEQYG
                     VTAEIASVIADGAFYYLDAPVKRMGAMNVPIPFSPVLEDLTVPTEETVFEMAKSLCGK
                     A"
     gene            6374..7699
                     /locus_tag="AC16CB_00720"
     CDS             6374..7699
                     /locus_tag="AC16CB_00720"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018226133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3341014.1"
                     /translation="MAIKTYGTMQVDWEQRIDFERLRRERLDRAKKLLKESEMGALLC
                     FDMNNVRYITATHIGTWAQDKVNRFTLLPQDDEPILWDFGSAAKHHQLYAPWLGEKRS
                     RAGISLLRGAMSPEMGRAEDVANKIWAELKERGLEKEPVGVDIAEVPVLFELQKKGLT
                     IVDGQQLMSEARVIKTQDEIALLTHAAAQVDAAYDELYRAMKPGMRENEAVGLVSKVL
                     YDLGSEYVEAVNAISGERCNPHPHVFSDRMLRPGDPVYYDILQSYMGYRTCYYRCFTI
                     GYASHAMVDAYKRCRDYLDAAIELVRPGRTTGEIASIWPKATEFGFPNEEAAFALQYG
                     HGVGLAIWEKPVISRLVSLDHPAEIKPGMVFALETYWPSSDGWSAARIEEEVVVTETG
                     HEIITRFPANDLMVAGHHYYTATGPLSGIRTHEPEMSMSVVELVESARG"
     gene            7700..9040
                     /locus_tag="AC16CB_00725"
     CDS             7700..9040
                     /locus_tag="AC16CB_00725"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIR3341015.1"
                     /translation="MATNVIMPALEMAQESGKLVRWLKREGETVTKGEPLMEIETDKV
                     TIEIEAPDSGILGGVLIKENDEVPVGQTIAWILAPGEKVPSIPVSEPQSGRVNTLQAL
                     PETKPQPTSAPVALEISPVARKMAEQHGIDLTQFKAKGKRIEKADIQAYLNAMQPAPA
                     RSSSTVAPTSPIVLASPKARRLASERGIDLSVLTGSGPDGAVLATDVPLSVERFSAAS
                     TSLETPGTVWRLMAERMSTSWTTVPHFYLVREVDASMLVDWRKSVTPTIEKRIGIKPT
                     FTDLLVKLIAFTLREHPRLNASWADGSIQWNQEINVGIAVAIDEGLIVPVIHGADGAS
                     LSEIATRRFDLIERARQKKLLPADISGGTFTLTNLGMYNIDAFDAIVNTPQAAILSVG
                     RIADRVVPVDRQVAIRPMMVMTLSLDHRVVDGARAAQFLDDLANLIEHPWGLMS"
     gene            9182..10495
                     /locus_tag="AC16CB_00730"
     CDS             9182..10495
                     /locus_tag="AC16CB_00730"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3341016.1"
                     /translation="MKSKFWKLLISITLMSMLIVACGGQPATEAPAPEAPAATEAPAP
                     TEVPPTEAPPPTEVPPTEEPEAEPIRIGASLPLTGRMSEPGTAAQQGYEVWAAIVNES
                     GGILGRQVELTIVDNASDQDTAVADYEKLITVDKVDLVVGPFSSFLVIPTSEVAARYG
                     YAFVEPAGGAPDVFNRGLTNIFFAQPAPGNQQAIPFTDYILALPDDQRPTTFAVVSQD
                     DPFTLGVMDGVKETLTGAGLELVFDEIYAPETTDFSSIAIQVADLDPDLIVGGTQLED
                     AVGQIRAYQEAGYQPRGAYFTTGPSLPGPFREGVGTATEGVFASISWFPEADEYQNAD
                     MVAKYTEMFGGNAGDIPEDVANAFTVGQVLHQAVENIDSLDNAALIEELHKGTYQTVV
                     GPLSFDEVGRPQGTFMLLQWQSDTFVIVGPGDRARADAIWPKPNW"
     gene            10655..11551
                     /locus_tag="AC16CB_00735"
     CDS             10655..11551
                     /locus_tag="AC16CB_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3341017.1"
                     /translation="MHLPSWNVLIQVTIIGILTGGVYALMATGLTLIFGVMRVINIAH
                     GAFLIFSAYISYWMFTKYGLDPFLSVIVSVPILFLIGIIFQRYVLSRVKGDPGLGVLL
                     TFAMALTLEGVMGSLWESTGRSIRTDYTSQVFAIHLSELTLRLPWMRVGGLVAAIAAL
                     GAIFLLLRSTNLGRAIRATIQNRSAAQLMGVNVERVQALTFGIGLASVAAGGALFSMI
                     WTFNSGSHEEWISKMLSIIVLGGMGSLPGTFVAAIIMGVAESVAAVVMTAYLSPIVFY
                     LILFVTLIFRPQGLFGVRIREG"
     gene            11553..12590
                     /locus_tag="AC16CB_00740"
     CDS             11553..12590
                     /locus_tag="AC16CB_00740"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3341018.1"
                     /translation="MTDFLARWWKYAVLLILLAYPWLFRAPYYHVLGFNVMLYAMMAT
                     SWNIMGGYTGYKSLGHSAFYGLGAYLVAIAANQLGWNPLLSAPLLAFVVALVAILLGW
                     IMLRTSGSAFVIATIAMLLIFRLTALNLREITKGAPGLSQPLPPWTPEFSRMPFYYYM
                     LLWLIISIFVSWYISRSRFGLGLVAIRDDEGKAEMVGVNTTLYKILAFGVSAYFVALG
                     GGVWSYFTSHISPVFAFDIIVGVDMILMTMLGGLGTLWGPVLGAFILIPSSDLILFSF
                     GSNTIYLAIIGALMITVIIFLPKGIIPTIQQRIQDHRAPRAAHEGAISMAEMHGLTDE
                     SASLVEENPHD"
     gene            12583..13317
                     /locus_tag="AC16CB_00745"
     CDS             12583..13317
                     /locus_tag="AC16CB_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012596637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3341019.1"
                     /translation="MTELLYTNGISRRFGGIQAVKDCNISVQAGSITGLIGPNGSGKT
                     TLFNLITGVYAPDEGEAYFDGKRITNLSPSKICHAGLSRTFQITRLFWEMTVLENMIV
                     PIRQVGLGSMLGPNMFKEEIDRAIGLLEHVGLTHLKDEPARKLSFGQQKLLEFAAILM
                     ASPTMVLLDEPAGGVNPTMIRFIMKLIQELNQDGVTFLVVEHNMGVVMELCNHVVVMD
                     QGTKLAEGAPEAIQANPAVLDAYLGN"
     gene            13320..14045
                     /locus_tag="AC16CB_00750"
     CDS             13320..14045
                     /locus_tag="AC16CB_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3341020.1"
                     /translation="MLLEIKDVVAGYLPDVNVLQGINIHVEPSELVCMIGPNGAGKST
                     VLRTISGLLQPKGGSIAFNGKEIGGLRPDLILHQGIAHVPQGHSSFPAMTVQENLLMG
                     AYILRNRAERNRRMGKVFEMFPILKERKNEKAGNFSGGQQKILEMGRALMLEPKMILL
                     DEPSLGLAPKTAKQVFDTIKSLNDEMGMTVLLVEQNAKSGLAIADRAYVLELGIERLE
                     GPASTLLNDPRVAELYLGGTLKS"
     gene            14050..14955
                     /locus_tag="AC16CB_00755"
     CDS             14050..14955
                     /locus_tag="AC16CB_00755"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3341021.1"
                     /translation="MSGALWAITAGVGFGLFQTFNRRAVRGMDVYVATFIQLLVSTIV
                     LLAIVLLTEDIGALLVAPFSVWVNFALAGILHFFLGWTFLNASQKRVGAARTSPLIGT
                     TPLFATAIAAVTLREFPGIWGIIGIGLIVLGAYVISQRSGDSREIEGNPQPIKAGWQA
                     SWLGLSAALCWALSPIFIRAGLEDLPSPLLGVTWGIAFSALAYGIVLLWQRDRWLGSE
                     ITGDAWMFKLLAGLLVGLSTWVRWIALDLAPVAIVIALTMISVPLVILLSPLVSGKQM
                     ESVTVSLWVGAGLILGGALLLTFIV"
     gene            14979..15962
                     /locus_tag="AC16CB_00760"
     CDS             14979..15962
                     /locus_tag="AC16CB_00760"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3341022.1"
                     /translation="MIIDTHAHIIVPEILQDAKPNEAWRPRVVWENGKQFVEYKSKRI
                     GSATREFVSVETILSEMDKSGVDAVLLCPWVSLVRYEAAPEESLDACQVQNDAMAELA
                     AKYPKKIAGLGMVPLQDVPLAVRELERLMKSGLKGVEIGTHVNGIYPGDERFRPFWEA
                     CEALGVFVFIHPVEGGGRAELRDYYMWNVIGNPLETTVAAGHLILSGVMDAYPRLNIL
                     LAHGGGALPYIHGRLDRGFKQRPEINKFISRPPTEYLKLFYFDTITHDPEVLRNLVDL
                     VGADHVLLGSDYPFDMGNEKPADLVRAAGLGADAEAKILGATAMQMLHWEV"
     gene            15963..16337
                     /locus_tag="AC16CB_00765"
     CDS             15963..16337
                     /locus_tag="AC16CB_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012417.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3341023.1"
                     /translation="MARLFTFEQLPHLHSTRDTRDRLDLVTDNVPVNAQAIRADRIIY
                     HPGDTAAKHYHTDCHHLFFVLYGNGILHVDGGSYRLAPGKVAVVAPNEIHWFENDTDE
                     NFAFVEFWGPPPKETVWITDDV"
     gene            16404..17636
                     /locus_tag="AC16CB_00770"
     CDS             16404..17636
                     /locus_tag="AC16CB_00770"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3341024.1"
                     /translation="MDMHMMDLSDEQRQMRAICREFVDNEVIPFIRANRENEWTTPPE
                     ERQPWELLKAADKLGLRALGVPEKYGGIQLEKQAQTFAIIAEELSRGDSGFADIISQL
                     WKVPVLLSNVAPEHLQDEWFPRFMDDPTFLMAHCLTEPRGASDRWLPYNVPEAGMHTK
                     AVLQDGHWVINGRKQFITNGYDAKLFVVYANTNPAVGIAEGTSSFLVPRDTPGLIPAR
                     VNEKMGARYMNNGEIIFENCRVPEDHLLVKDIALRKAGVYFRPGKILQAAKNLGVGVA
                     AYEDTAAYVQQYVQGGKILIKHQAVANKLADMAIKIETTRTFTRYAARALDEGASDAE
                     KLCLMAKTYAADAIFEVCKDAMELHGGAGVMVEVGIEKHFRDAAVYLHMDGTQDIHRF
                     KIIKTIYPETAGVYAGAD"
     gene            17659..>17945
                     /locus_tag="AC16CB_00775"
     CDS             17659..>17945
                     /locus_tag="AC16CB_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3341025.1"
                     /translation="MSKPLGVTVIGTGMWGKHMLAAVKRTPSLRLVACYSRDVAAREA
                     VAAEFGCAAPETFEQAINWDGVQGVLLVTPNHVHAEQARECARSGKHIFVE"
BASE COUNT         4234 a         4473 c         4994 g         4244 t
ORIGIN      
        1 gagagcggtt cgacttgtga ccccgacagg actcgaacct gtaaccaatt gatgaagagc
       61 gggaccgcaa ccccatatat tgcgctttat aagtcataga atcgataatg gagattgatt
      121 ctatgcaaat tgcaccacaa ttgcaccaac aaggaaaggc gttttgtaac ccggtcctta
      181 atatttactg tttctttttc taaaaatgct atttcgccag gtgtcgtaac gaaccccatt
      241 ggcgtaggat cagcacgcat aggctcgatc acagttgccg cggtctggat attcctgctg
      301 gccttaatca tcttttaccg cgaggaggtt aacctgcgct ggcccgatct tccagacaag
      361 ccaaatcttc ttcaaagttt taccttaccc aactgatccc attctgaagc aagcatgggg
      421 attcagatca tcaagcatgg caattgggaa tccccatagc tgaggatgtt gactacatca
      481 agatacatcc ttataatctc taaaatttta tgagacccta ggaggtgcgc attggatatt
      541 ggctttgttg gattgggtgt gatgggggaa ggtattgtac cgcgccttct ggcagctgga
      601 cactctgtca caggttggaa tcggactcag gggaaagcgg aacctctgat caaactgggg
      661 atgcattggg ctgatagtcc gcgcgaagtg gcaaaacaat cggagattgt gttttccatt
      721 gtcacagatg cagcggctgt cagagccgtg gcgcttggga ccgatgggat tatcgccggg
      781 cttcgggaag acgggatata tttggacatg agcacgatcg ccccggatgc aagccgtgca
      841 attgccgctg aattcgaatc ggcgggcttg accatgttgg atgcgcctat ctctgggagt
      901 cccattacgc ttgcacaggg taaggcctcg atcatggtgg gcggcaataa ggacgccttt
      961 gagcgagtcc acccggtgtt attggatatc ggagcgaaag ccatgtatat tggtgggaat
     1021 gggctggcgg tccagatgaa gctcgccatc aatctcgtgc ttatggtcga ggtgattgcc
     1081 tttggcgaag gcatcgcgct cgccgaaaag gggggcgtaa agcgtgaagt tgccgttgag
     1141 gcgatgctca acagcgtagc cgcttccccg gttctaggtt accgcggccc gtttattctg
     1201 gaagggaaca tgcccgaagt cccgctggca gatgttacct tacagcaaaa ggatatgata
     1261 ttggtcttgg atttggggcg caagctgggt tctccggtgc cgcttgcagc ggctgccaat
     1321 gaaatgatga atgcctgccg aggccttggc attgaccacc gagattttgt caccgcccat
     1381 gaagtgtatc gggttttagg aggaatgtta aaatgaagcc ttatcgtacc aatttgcgcg
     1441 atgtacccta tgtcgaaggg ttatcccccc gggatggctg gatcaatatg caggttcaat
     1501 ttctgatcga tgagaagaca gctaattcgg ataaactggt cctcggctgg acagtcttgc
     1561 cgcctggggc gcggcacgat cgtcatcgtc atttccaggc tgatgaattc ttgctggtgc
     1621 tcaaaggtca tggagagatc tataccgatg agggcaatga gccggcgggc gagggagatg
     1681 ttgtgtatac aacccgcggt cactggcatg gtttcaacaa tacctcccat gaggaggttg
     1741 tcctggtatg gggttggagc ggagccggct ccctggaatc ggctggctac gaagtctcgc
     1801 acgaccattc gcattccgat caggcataaa cggtgaatgt agctggttac gattcgcgca
     1861 tcctgcgcgg gatggagaaa ctcctaaagc tacgccaggg tcgattggac gctggtgaga
     1921 aatccatcgg gtggaaagtt ggatttggag cgcctgcctc attggaagcc ttaagacttg
     1981 acgcacctct ggtgggcttt ctaacggaca aagtgctcct ggattccggt tctgcgctct
     2041 cgattgctgg ctggacgaag cctgccgcgg aacccgagat cgctgtctat cttaataggg
     2101 accttcctgg ggtttcagac agggaaacaa tccaggcagc catttccgca atagggcctg
     2161 cgattgaaat tgcggatgtg cacttcccac caaacgatgt cgaagcgatt ttagccggga
     2221 acatctacaa ccgccatgtt atcttaggaa aatcggataa ctcgcgcgcc ggctgtacat
     2281 tggatgggtt ggtgggccgg atttaccgca atggacaaga aattgccacc gtgaccgatt
     2341 tacaggtgtt gacaggaaac ttcattggca ttgtgcaaca cgtggccaac ctattagcat
     2401 cccttggaca aatgctgcgt tctggggatg taattatcac gggctcaatc gtgccgccgc
     2461 tgtggatcga aagagatgaa gaaattcgat tcgatcttga tcctatagat aagctaacaa
     2521 ttagacttga aaattaatat ctacacctag cgctcggcat taccgaagta gatgattttg
     2581 aaacacaccg cagaaaactg cggtgtgttt cactaaaaag aaaccgttag gcgtttgtga
     2641 aataattcac catatttggc tattgacacc cgtttttgtc gtgatataat ggtatggtca
     2701 tcataccact tgtgaaaaac ctctcataat gtatcaacct atccagtctg aacgcctata
     2761 tgaacggatt gtagaccaga tcgaacgcag gattttagcg ggagatctga agatagggga
     2821 tcaattgcct gctgaacgag agcttgcaga acagttcgct gtcagccgaa cagcggtgcg
     2881 tgaggccatt aaggctttga gagagaaagg tctggtggag attcgtttag gccgggggac
     2941 attcgtgacc agtggtgcgg caggtgcagt tcgacgttcg ctaggtttgt tgctaaaaac
     3001 tgaaaatggc tttgttaatc tggaagaggt gcgggagatc ctggaacccg aaatcgctgc
     3061 gctggctgct actcggatta gcgaagaata tattaccgcg atgacggagg ccgttgaaac
     3121 gatggatacc gccctggata acgtcgagat ttttgtagag gccgacctcg acttccatct
     3181 ggcgctggcg gaagcaaccc aaaacccaat tattccatcc ttaatggatt cgatcattga
     3241 tttgctccgc gaacagcgca agcggacagg gaatgtggaa ggtggcctgg cacgagggca
     3301 atatcaccat aaaaagatcc ttgaagctgt catacaacgc gacccacagg cagcccgcaa
     3361 ggcaatgcaa gaccaccttc aacaagtgcg cgaagatact tacgcctcag ccaaaaacac
     3421 ggactgaggc ataatttcgg aggattgcat ggcaaacctg ggatttatcg gactcggtgt
     3481 aatgggtgga cggatggtaa agcgtcttct agacgctggt cacagcgtaa cgggctataa
     3541 ccgaactcgt tcaaaagccc aatggctgtt ggaagcaggc atgacctggg gtaatacgcc
     3601 tcgtgccgtg gccaaatctg ctgacatcat tttaagtatg gtcacaaaca ccgaagctct
     3661 tcacagcata gccgaaggcc ccgatggtct attagcgggc ttaagtgctg gcaagattta
     3721 tgtggatatg agcaccgtca gtccggcctt cagtcgggaa ttggcaaagg aggttgctat
     3781 aaaaggtgct tccatgctgg atgctcctgt atcaggcagt gtggtgactc tcgaagaggg
     3841 tcgattgtcc gtgatggttg gcggtgacgc agagacattc gaaaaggcaa aaccgatttt
     3901 acaagatatt gggcccaaag caacgcacgt gggcgccaat ggtctcgccg tgacaatgaa
     3961 aatcgcaacc aatctcagtc tggcagtgca aatgctggct ttcagcgagg gtgttctgct
     4021 ggctgagaag agtggcatca agcgcgagat tgcagtagaa gtgctggtta acagtgttat
     4081 cgcttcgcca atggtcaagt atcgcggtcc gttcgtcctg gagatgccag atgaagcctg
     4141 gttcgattgt tatatgatgc agaaggatat gaacctagca ctggaactgg gacgcgagct
     4201 gagcattccc ctgcccacca ctgctgtaac gaacgaattc ctttctgcag cccgaggtat
     4261 ggggcttgaa aaacaggatt ttgcgatcat gttcgatgtg ctggcgcaaa tgtcgggagt
     4321 gaagtaatgg ccaatgaaac gcaagtcact gccaacccca aaccagagac gctagacgtc
     4381 gagcaatggc tgcatgccta cgagcagatg gccaagatcc gcgagttcga ggagaaggcc
     4441 aatgagttat acatgaaggc actgatgccc ggacttaccc atttgtattc gggtcaagag
     4501 gcagtcgctg taggggtctg tgaagctttg cggcgggacg attacattac cagcacgcac
     4561 cgcggtcacg gtcattgtat tgcgaaaggt gcttcggttg acttgatgtt cgctgaactt
     4621 ctgggcaaag aagccgggta ttgccgcggt aaaggcggct caatgcacat tgctgatccc
     4681 gaatctggca atctgggggc gaacgccatt gtgggtggca gtgccggaat agcgactggc
     4741 gcagcattat cggccaagat gcgtggcacg gatcaggtag ccgtttgttt tttcggggag
     4801 ggggcattgg ggcaaggtct tttgtatgaa gtcatgaata tggcatcgtt gtggaaattg
     4861 cccattatct acgtctgtga aaataacctg tataacgaat atacccatta tagcgaggcg
     4921 cttgcagggg aggttaccga gcgcgccaga gcatttggga tccacaccga gagcatttac
     4981 ggtcaggacg tgcagctggt ctatgagacg atgcggaaac tggttgagcg cgcccgccgc
     5041 ggtgaaggac ctgcattcct agagtgcctg acctaccgtt attacggtca ccatgtgggg
     5101 gatatcaatc gtacatatta ccgctcgaag gaagaagagc aagaatggcg agaagtccat
     5161 gatccgttga aactgttaga ggaaaaatta gttgcatcaa agctcactga ccaagatgtg
     5221 cttgagcgca tttacgcaga tgcaaaaaac gaagtggaag caggtgtaca gtttgccatc
     5281 gacgcgccat acccagatca gagccaggtg accgaggatg tttatgcctg acattaaagt
     5341 ttcacaatgg gaggcagcac aataaatatg gctgagcgtg aaataacgtt tgcagaagcg
     5401 atccgcgagg cactggctga agaaatgcgt cgcgatcaga atgtttttat tatcggtgag
     5461 gatgtagcag aagcgggcac gccgttcaag gttttgtctg gtctggtgga agagttcggc
     5521 aagaatcgtg tgttcgactc gccaatctca gagcctgggt ttactggcat gggggttggt
     5581 gcggcgatga cgggcatgcg cccggtagtg gacatcatgt tcggcgattt cctgaccctt
     5641 gcaatggacc agattgtcaa ccaggctgcc aaagttcact acatgtccgg cggcaagctc
     5701 aaggttccga ttgtgttccg caccaccatg ggcgccacgc gccgttcagc tgcccagcat
     5761 tcccagagct tacaggcctg ggtcagccat gtgccagggc tgaaggtcac catgccatcc
     5821 acgccttacg atgccaaagg tttgctcaag accgccatac gtgacgagaa ccccgtggtc
     5881 ttcatcgaag acaaaatgat gtacaagatc aaaggtcccg tcccagcgga ggattataca
     5941 atcccattcg gggtagcaga catcaagctg caaggcgacg acattacatt ggtggcgacc
     6001 agttccatgg tccaggtggc tcttggagca gcagaaaaac tcaaggatat tggcatcagc
     6061 gccgaggtca ttgatccccg caccaccacc ccgctcgaca aacaaacgct gatcgaatcc
     6121 gccaaaaaga cttcacgggc catcgttatt gacgaaggct atgagcaata tggcgtcacc
     6181 gccgagattg cgtcggtgat tgccgatggt gcattctatt acctggatgc accagtaaaa
     6241 cgcatgggcg ccatgaacgt acccatcccg ttctcacctg tgctagaaga tttaaccgta
     6301 cctactgaag aaaccgtttt cgaaatggca aagtcacttt gtggcaaagc ctagtaaacg
     6361 aggagaaatt gaaatggcta taaaaaccta cggaaccatg caggttgatt gggaacagcg
     6421 catagacttc gaacgcttgc gccgtgaacg tctcgaccgg gcgaagaagc tactgaaaga
     6481 atcggaaatg ggcgcgctgc tctgcttcga tatgaacaac gtgcgctaca taactgccac
     6541 tcacatcggc acttgggcgc aggacaaggt caaccgcttt acattgttgc cccaggatga
     6601 cgaaccaatt ctgtgggact ttgggtctgc cgccaaacac caccagcttt acgctccctg
     6661 gctgggagag aagcgctcgc gggctggtat ctcgctgctg cgcggggcga tgtcgcccga
     6721 aatgggccgc gctgaagacg tggcgaacaa aatctgggcc gaactcaaag agcgtgggtt
     6781 agagaaagaa ccggtgggtg tggatatcgc tgaagtgccg gttctcttcg agctgcagaa
     6841 aaaggggttg actatcgttg acggtcagca gttgatgtct gaggcgcgcg tcatcaagac
     6901 gcaggatgaa attgctctac tcactcacgc cgcggcccag gtcgatgcag cttatgatga
     6961 gctgtaccgg gcgatgaaac ctggcatgcg cgagaacgaa gccgttgggc tggtctccaa
     7021 ggtgctgtac gacctcggct cggaatacgt agaagcggtg aacgctattt ctggtgaacg
     7081 ctgcaacccg cacccgcatg tcttctcaga ccgcatgtta cgccccggcg acccggtcta
     7141 ctatgatatt ttgcagtctt acatgggcta ccgcacttgc tactatcgct gtttcacaat
     7201 tggctatgcc tcgcatgcca tggtagacgc atacaaacgc tgccgcgatt atctcgatgc
     7261 ggctatcgaa ctcgtccgac ctggacgcac cacaggcgag attgcgtcaa tttggccgaa
     7321 agctactgaa tttggattcc ctaacgagga ggctgccttc gccttgcagt atggacatgg
     7381 cgtcggttta gccatctggg agaagccggt tatcagccgc ctcgtctcat tggaccatcc
     7441 tgctgaaatt aagccaggta tggtgtttgc gctcgaaacc tactggccat cctcagacgg
     7501 ctggagcgcg gcccgtattg aggaagaagt cgtggttacc gagaccggtc acgagatcat
     7561 cacccgcttc ccagccaatg acttgatggt agccgggcat cactattaca cggcgaccgg
     7621 ccccctgtct ggaatacgca cccacgagcc ggagatgagc atgagtgttg tggaactggt
     7681 agagtcggca agaggctaga tggcaaccaa tgtgataatg cccgcccttg aaatggctca
     7741 agagtcgggc aaactggtga gatggctcaa gcgtgaagga gaaaccgtca ccaaaggtga
     7801 gccccttatg gaaatcgaga ccgataaggt gactatcgag atcgaggcgc cagattcagg
     7861 cattctaggt ggggtgctta tcaaggagaa cgacgaggtg ccggttggtc aaaccattgc
     7921 ctggatcctg gcacctggtg agaaagtgcc atccatacca gtgagcgaac cacagtctgg
     7981 gcgggtcaac accctgcaag ctctccctga aaccaaacca caacccacat ccgcgcctgt
     8041 cgcgttagaa atttcaccag tggcgcgcaa aatggcagag cagcatggga tcgacctgac
     8101 acagtttaag gctaaaggga aacgaatcga aaaagccgac atccaggctt acctgaacgc
     8161 catgcaacct gctcctgctc gcagttcatc taccgttgca cccacttcgc ccatcgtcct
     8221 tgcttctcca aaagcgcgca ggctggccag tgagcgcggc atcgacctgt cagtattgac
     8281 cggctccggg ccggacggcg cagttttggc caccgacgtg ccgttgagcg ttgaaagatt
     8341 ctcggctgca tcaacaagtc tcgaaacgcc gggaacggtc tggcgcctga tggcagagcg
     8401 tatgtccacc agttggacaa ccgtgccaca tttctacctg gtccgcgaag tggatgccag
     8461 catgctggtt gattggcgaa aaagtgtcac gccgactatt gaaaaacgca ttggaatcaa
     8521 accaacattt acagatctac tggtgaagtt gatcgcgttc accctgcgcg agcatccccg
     8581 tctgaacgca tcttgggcag atggcagcat ccagtggaat caggaaatca acgttggcat
     8641 tgctgttgct attgatgagg gattgatcgt cccagtgatc cacggagcag atggtgcctc
     8701 attaagtgag atcgccacac ggcgctttga tctgatcgaa cgcgcccgcc agaaaaagct
     8761 gctgcctgct gacatctcgg gtggcacttt tacattgacc aacttgggca tgtacaacat
     8821 cgatgccttt gatgccatcg tcaatacacc gcaagccgcc attctatcag tgggacgcat
     8881 cgctgatcgg gttgtgccgg tggacaggca agtggcaatc cggccaatga tggtgatgac
     8941 actatcactc gatcatcgtg tagtggacgg ggccagggcg gcgcaattcc tggacgacct
     9001 ggccaatctg attgaacacc cgtggggact aatgagttga aatacctcac gtggtgggca
     9061 caatctcatg ctgctatcct cgaatatcgc aagtatggaa ggaggagtgg atggattaac
     9121 tcatcgataa tctgactagc ttataaactc aaaatctaat catatccaaa ggagagaaaa
     9181 aatgaaatcc aaattctgga aattgttgat cagtatcaca ctcatgagca tgctgatcgt
     9241 agcttgcgga ggacagcctg caactgaagc gcccgccccc gaagcaccgg cagctactga
     9301 agcaccggca ccgaccgaag ttcccccaac cgaagcgccg cctccgaccg aagttccccc
     9361 aacagaagaa ccggaagctg aaccgattag gatcggtgca tctctgcctc taaccggccg
     9421 gatgagcgag ccaggcacgg cagcccaaca aggctatgaa gtatgggcag ctattgtcaa
     9481 tgaatccggc ggcattcttg gtcgccaggt cgaattgaca attgtggata acgcctccga
     9541 ccaggatact gcggttgctg actatgaaaa actgatcacc gtcgataaag tagacctggt
     9601 tgtcggtcca ttctcgagct tcctggttat cccgacctca gaagtggcgg cacgctatgg
     9661 ctatgccttt gttgagccgg caggcggcgc ccccgatgtc tttaaccgcg gcttgaccaa
     9721 tatcttcttt gcccagcccg ctcctggaaa ccagcaggcc atccccttca ccgactatat
     9781 tctggcattg cctgacgacc agcgcccgac gacctttgct gtggtcagcc aggatgatcc
     9841 cttcacactg ggcgtgatgg atggcgtaaa agaaaccctg actggtgccg gcctcgagtt
     9901 ggtctttgat gagatctatg ctcctgaaac cactgacttt tcatccatcg ccattcaggt
     9961 tgccgatctc gatccagact tgatcgttgg cggcacgcag cttgaagatg ctgttgggca
    10021 aatccgcgcc taccaggaag ctggctatca gccgcgcggc gcctacttca ccactgggcc
    10081 gtcactgcct ggcccattcc gtgagggtgt gggcactgca acggaaggcg tattcgcctc
    10141 catttcctgg ttccccgagg cagatgagta tcagaatgca gacatggtag ccaagtacac
    10201 cgaaatgttt ggcggtaacg ctggtgatat ccccgaagat gttgctaatg ccttcactgt
    10261 cggacaggtg ctacaccagg ctgtcgaaaa tatcgactcc ctggataacg cggctctgat
    10321 cgaagagctc cacaagggaa cgtaccagac cgtggttggt ccgctcagct ttgatgaagt
    10381 cggccgcccg caaggtacct tcatgttact ccagtggcag agtgatacct tcgtgatcgt
    10441 cggccctggc gatcgcgccc gtgcagatgc tatctggccg aagcccaact ggtaaattat
    10501 agtttgaccg tcgattgggg ctgtctataa gttgtaggac gatttgccaa acctccgcct
    10561 gtaggtcggg atactaaccc gacctacagg caggagcact ggctgttcgt tcagttcttt
    10621 tgagacagcc ccaactttat ctaggagaga gcaaatgcat cttccttctt ggaatgtact
    10681 catccaggta acgatcatcg gaatcctgac gggaggggtt tacgccctga tggctaccgg
    10741 tctgacgttg attttcggtg tgatgcgcgt gatcaacatc gctcatggtg cattcttgat
    10801 ctttagcgct tacatttcct attggatgtt tacaaagtat ggtctggatc cttttctttc
    10861 ggtcattgtg tctgtgccaa ttttattttt aattggaatc atctttcaac gctatgtgct
    10921 ttctcgggtc aagggtgatc caggcctagg cgttttgctc acctttgcca tggcactgac
    10981 actggaaggg gtgatgggtt ctttgtggga gtcgactgga cgctccatcc ggacagacta
    11041 caccagtcaa gtgtttgcaa tccacctttc cgaactgact ttgcgcctcc cctggatgcg
    11101 cgtgggtgga ttggttgctg ccattgccgc acttggagcg atttttctct tattgcgttc
    11161 cactaacctt ggtcgcgcta tccgcgcaac gatacagaat cgctcggccg cacaattaat
    11221 gggggtaaac gtggaaagag ttcaggcact cacctttggg attggcctgg cctccgtagc
    11281 ggcaggtggc gcgctattta gtatgatctg gaccttcaac tctggcagtc atgaagagtg
    11341 gatctcgaaa atgctttcga tcattgtgct tggtggcatg ggtagccttc cggggacatt
    11401 tgtggctgcg attattatgg gcgtcgctga gagtgtggct gctgtagtta tgacagccta
    11461 tctctcaccg attgtttttt acttaatctt gttcgttaca ctgatattcc gcccgcaagg
    11521 tttgttcggc gtacggatca gggagggttg agatgacgga tttcctggct cgttggtgga
    11581 aatatgctgt cttgttgatt cttctcgcgt acccctggct gttccgagcg ccctactatc
    11641 atgttttggg attcaatgtc atgttgtatg cgatgatggc aaccagttgg aacattatgg
    11701 gcgggtatac gggttacaaa tccctcggcc atagcgcctt ctatgggctg ggtgcctatc
    11761 tagtggccat cgcagccaat caactgggtt ggaacccatt gcttagtgcg cctttgctgg
    11821 cgttcgtcgt cgccctggtc gctatcttgc tggggtggat catgctgcgc acaagcggtt
    11881 ctgcttttgt cattgcgacg attgctatgc tgctcatctt ccgcctaaca gccctgaatc
    11941 tccgagagat taccaaaggc gcaccaggtt tgagccaacc actcccaccc tggacgcctg
    12001 aattttctcg catgccgttc tactattaca tgctgttatg gctgattatc tccatctttg
    12061 tctcctggta catcagccgc tcccgctttg ggctgggatt ggttgccatt cgagatgacg
    12121 aaggtaaagc agagatggtg ggcgtcaaca caaccttgta taagatcctt gccttcggtg
    12181 tcagtgccta ttttgtggcg ctgggcggtg gcgtatggtc ctatttcacc tcccatatca
    12241 gcccggtttt tgcgttcgat attatcgtcg gggttgacat gatcttgatg acgatgctgg
    12301 gtggtctggg cacgctttgg ggacctgtgc tcggcgcatt catcctcata ccatcctcgg
    12361 atctgatcct attcagcttc ggttcaaaca ccatctacct ggcaattatt ggcgctttga
    12421 tgataacggt gatcatcttt ttgccgaagg gcatcatacc aaccatacaa cagcggatac
    12481 aagatcaccg tgccccacgc gcagcgcatg agggagcaat ttcaatggcc gaaatgcatg
    12541 gcttaacaga cgagtccgcc tccctcgtcg aggagaatcc ccatgactga attactctac
    12601 acaaatggga tttctcgtcg tttcggcggt attcaggctg tcaaggattg taatatctcg
    12661 gttcaagcgg gcagcatcac cgggttgatc ggtccaaacg gctcgggaaa gaccacctta
    12721 tttaacttga tcacaggcgt ctacgcccct gatgaagggg aagcctattt cgacgggaag
    12781 cgcattacga acttatctcc cagcaaaatt tgccacgcag gactcagccg aaccttccag
    12841 atcaccaggc ttttctggga aatgacagtg ctcgaaaaca tgatcgtccc catccgccag
    12901 gttggtttag gttcgatgct tggccccaac atgttcaaag aagagatcga tcgcgcaatt
    12961 gggctcctgg aacacgtcgg gctcacgcat ctgaaagatg agcctgcccg caagctgtct
    13021 tttggtcagc agaagctttt ggaatttgcc gcgatcttaa tggcaagtcc caccatggtt
    13081 ttgctagatg aaccggcggg gggagtaaac cccacgatga tccgtttcat catgaaattg
    13141 atccaggaac tcaaccagga tggcgtgact tttctggttg ttgagcacaa catgggcgta
    13201 gtcatggaac tttgtaatca tgtcgtcgtg atggatcaag gcacaaaact tgctgaaggc
    13261 gcgccagaag cgatccaagc caacccagcc gttttagacg catacctagg aaattaacaa
    13321 tgttactcga gataaaagat gtcgtcgccg gatacttgcc ggacgtgaat gtcctccaag
    13381 gcatcaatat ccatgtagag ccaagcgaac tggtatgcat gatcggtcca aatggcgcag
    13441 gcaaatccac agtactgcgt acgatctctg gcctgttgca acccaaaggc ggaagcattg
    13501 cttttaatgg aaaagagatt ggaggtctac gtcctgattt gatcttgcat caggggattg
    13561 cccatgtccc gcaggggcac agctcattcc cagcgatgac cgttcaggaa aatctgttga
    13621 tgggggctta tatattgcgg aacagggctg aacgtaaccg ccgtatgggc aaagtgtttg
    13681 aaatgttccc cattctgaag gagcggaaga acgaaaaagc cgggaatttt agcggcggac
    13741 agcagaagat cctcgaaatg ggacgggcac tgatgctcga accgaagatg atcttacttg
    13801 atgaaccttc gttgggactg gccccgaaaa ccgccaaaca ggtttttgat accatcaaat
    13861 ccctgaacga tgaaatgggc atgacagtgt tgttagttga gcagaatgcc aaaagcggat
    13921 tggcgattgc tgatcgcgct tacgtgctgg aactggggat cgaacgtttg gaaggccctg
    13981 cttccacctt gctcaacgac cctcgcgtgg ccgagcttta tctgggtggc acactaaaga
    14041 gctaaaccta tgagtggagc tttgtgggcc atcaccgccg gcgtgggttt tgggcttttc
    14101 cagactttta accgtcgcgc ggtgcgtggg atggacgtat atgtcgccac gttcatccag
    14161 ttgctggttt caacgatcgt cctcctagca attgtcctac tgacagagga tataggcgcc
    14221 ctgcttgttg ctccatttag cgtttgggtg aattttgctc tagccggtat attgcatttt
    14281 ttcttagggt ggacttttct gaacgccagc cagaaaagag ttggagccgc gcgcacaagt
    14341 cccttgatcg ggacaacccc gctctttgca accgccatcg ctgcagtgac gctgcgcgaa
    14401 tttcccggta tttggggcat tatcggcatt ggcctgatcg tactaggggc ttacgtgatt
    14461 tctcagagat caggcgattc gagagaaatc gaaggcaatc cacaaccgat caaggctggc
    14521 tggcaggctt cctggctggg gcttagcgct gctttatgtt gggcactcag cccaattttt
    14581 atccgagctg gcttggaaga cctgccctct cccttgttag gtgtcacatg gggcatcgca
    14641 ttcagcgcgc tagcgtatgg gattgttctg ttgtggcagc gagatcgctg gctcggatcg
    14701 gagattactg gcgatgcctg gatgtttaag cttctggcag gtttgctggt tggcttatcc
    14761 acctgggtgc gttggatcgc gctcgatcta gctccggtcg caattgtgat cgcactgacc
    14821 atgatatcgg tgccgctggt gatcttgctc tccccactcg tgtctggcaa gcaaatggaa
    14881 agtgtgaccg tctccctgtg ggtaggagca ggtctgattt tgggtggcgc gttgctgttg
    14941 acttttatcg tctgaatcga gaaggagttt ctagatacat gatcattgat actcacgctc
    15001 acattattgt ccccgagatt cttcaagatg caaaacctaa cgaagcgtgg cgtccgcgcg
    15061 tggtgtggga gaacggcaaa caatttgtag agtacaaaag taagcgaatt ggttctgcga
    15121 cacgcgagtt cgtatcggtg gaaaccatac tgtccgaaat ggacaaatct ggcgtggatg
    15181 cggtcttgct ctgcccgtgg gtctcactgg tgcgctacga agctgcgcca gaagagtcgc
    15241 tggatgcatg ccaggtacag aatgatgcta tggctgaact ggcggcaaaa tatcccaaaa
    15301 agatcgccgg ccttggcatg gtgcctctcc aagatgtccc actggctgtg cgcgaactgg
    15361 aacgtttgat gaagtctggg ctaaagggtg tcgagatagg cacgcatgtc aacggtatct
    15421 atcctggaga cgagcgtttc cgtccatttt gggaagcctg cgaagcgctt ggtgtgttcg
    15481 tatttatcca tccagtggaa ggtgggggac gcgccgaatt acgcgactac tacatgtgga
    15541 atgtgatcgg caacccgctg gaaaccaccg tcgctgcggg acatctaata ctgtctggcg
    15601 tgatggacgc atatccgcgt ctgaatattt tgcttgcaca cgggggcggg gcactgcctt
    15661 atatccacgg gcggctggac cgcggcttca agcagcgccc ggagatcaac aaattcatct
    15721 cccgcccacc gactgaatat ctcaagctat tctatttcga taccatcact cacgatcccg
    15781 aagtgctgcg caacctagtg gatctggtgg gcgccgatca tgtcctgctc ggttcagatt
    15841 atccttttga tatggggaat gagaagccgg cagatttggt gcgtgcggct ggtcttgggg
    15901 cagatgctga agcgaagatc ctgggtgcaa ccgcgatgca aatgctgcat tgggaggttt
    15961 agatggctcg tctcttcacg ttcgagcaat taccacatct gcactccact cgcgatactc
    16021 gcgaccggct ggaccttgtg acagataatg tcccagtcaa tgcgcaggca atccgcgctg
    16081 accgcattat ttatcatccc ggcgatacgg cagccaaaca ttaccacact gactgccatc
    16141 acctcttctt tgtgctgtac ggcaatggca tcctgcacgt ggacggggga agttatcgcc
    16201 tcgccccagg caaggtggct gtcgtcgccc ctaatgaaat tcactggttt gagaatgaca
    16261 cggatgaaaa ttttgccttc gtcgagttct ggggaccacc gccgaaagag acggtttgga
    16321 taacagatga tgtctgaact tgggagtcac acgcctgagt tgcaggcgtt ctatataacg
    16381 cgaaaaacac aggaggtacg cggatggaca tgcacatgat ggatctgtct gatgaacagc
    16441 ggcaaatgcg tgcaatatgc cgtgagttcg ttgacaacga ggtaattcct tttatccgtg
    16501 ccaatcgaga gaatgagtgg acaaccccac cagaggaacg ccaaccctgg gagctgctga
    16561 aagcagctga taagctcggc ctgcgcgccc taggtgtgcc ggagaaatac ggtgggatac
    16621 aactggaaaa gcaggcacag acattcgcaa tcattgcgga agaactgtcg cgcggagatt
    16681 cgggctttgc cgatattatc agccagctat ggaaagtgcc cgtcctgctt agtaacgtgg
    16741 cgccggaaca tctccaggac gagtggttcc cgcgcttcat ggacgatccc acattcttga
    16801 tggctcactg cctcactgag ccgcgcggtg cttctgatcg ctggctcccc tataacgtgc
    16861 ctgaagccgg catgcacacc aaagctgttc tacaggatgg acactgggta attaatgggc
    16921 gcaaacaatt catcaccaac ggttacgatg cgaaactgtt tgtggtctat gccaatacta
    16981 accctgctgt cgggattgct gaagggacaa gcagcttcct ggttccacgt gatacacctg
    17041 gtcttatccc tgcccgcgtc aacgagaaga tgggcgcgcg ctatatgaac aatggcgaga
    17101 tcatttttga aaactgccgg gttcctgaag atcacctgct ggtcaaggac atagcccttc
    17161 gcaaagcggg tgtatacttc cgcccgggga agatcctgca agcggcaaag aatttgggag
    17221 ttggtgttgc tgcgtatgag gacactgccg catatgtcca gcaatatgta cagggtggaa
    17281 agatactcat caagcaccag gcggtggcga acaaacttgc tgacatggca ataaaaatcg
    17341 agacgacgcg cacattcacg cgctacgccg ctcgcgcctt agacgaaggc gcttcggatg
    17401 cagaaaaact gtgcttgatg gcgaaaacct atgcggcgga tgccatattt gaggtctgca
    17461 aagatgccat ggagttacat ggcggcgctg gcgtcatggt cgaagttggc atcgaaaagc
    17521 attttcgaga cgcggctgta tatttgcaca tggatggcac gcaggatatc caccgcttta
    17581 agatcataaa aaccatttac cctgaaactg caggggttta tgctggtgcc gattgaccat
    17641 gaccaggatg atagtgtcat gagcaaacca cttggcgtga ccgtgatcgg cacaggtatg
    17701 tggggaaaac acatgctggc ggctgtcaag cgaacgccgt ccctgcgcct ggtagcctgt
    17761 tacagccgcg atgtggctgc ccgagaagct gtagcagctg agttcggctg cgctgcgcca
    17821 gagacattcg agcaggccat caactgggac ggagtccagg gtgtgctgct ggtcacgccc
    17881 aatcatgtcc acgcggagca agctagagaa tgtgctcgat cgggaaagca catcttcgtc
    17941 gagaa
//
LOCUS       DBNGSX010000025         3959 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1248195, whole genome shotgun sequence.
ACCESSION   DBNGSX010000025 DBNGSX010000000
VERSION     DBNGSX010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1248195"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="AC16CB_00780"
     CDS             complement(<1..270)
                     /locus_tag="AC16CB_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341026.1"
                     /translation="MVNHRATTQIPPLVRRAQNLAEQMEYSGSCSNETGRLLQLLTSS
                     FLSGVIGEIATGCGVGSAWIASGLAPSTSFFTVEIDDARAAATRAL"
     gene            complement(297..914)
                     /locus_tag="AC16CB_00785"
     CDS             complement(297..914)
                     /locus_tag="AC16CB_00785"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR3341027.1"
                     /translation="MDTDSGQINHFTARFEQALVYATRLHNGQSRKGTDIPYVAHLLA
                     VTALVIEWGGDEEMAIAALLHDAVEDQGGLETLTEIRRQFGERVARIVEGCTDSYLQP
                     KPPWRGRKEAYLAKLRGEPHDVRIVSLADKVHNARSILSDLRRNGLSAFDRFRGGRDG
                     TLWYYRSLVEVFTEVEESPLVAEFSRLVDEIEELANPEYKKHCGQ"
     gene            complement(923..2119)
                     /locus_tag="AC16CB_00790"
     CDS             complement(923..2119)
                     /locus_tag="AC16CB_00790"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019588617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3341028.1"
                     /translation="MALTSIHITNEEYRQRADALLATIQKEHLSEVVLFDNYYILYYT
                     GFAFVPTERPMAFLMNAKGERCLFVPRLELEHAQANANVDQVEYYIEYPFEPHPMEIL
                     YQLMKRLGFGEDYGADQDGYPWILGFRGPTLSDLTGWKPRRVTGFIEDQMAVKSPAEI
                     ALIKESVKWGHLAHVLLQRYTRVGESETQVSLRASMEATLTMLDTIGPIYRAQSVFST
                     GAEANYRGQIGRNAAIPHALANNLTFQVGDVLVTGAGAPVWGYNSELERTMVVGPPSD
                     MQKRMFDHMVALQDIAFEALIPGQSCSTVDKTVRAYYESHDLMDYWKHHVGHTIGLRY
                     HEGPFLDLGDATVIKPGMVFTVEPGLYVPELGGFRHSDTVLITEDGNEVLTYYPRDLD
                     SLTIPI"
     gene            complement(2181..2609)
                     /locus_tag="AC16CB_00795"
     CDS             complement(2181..2609)
                     /locus_tag="AC16CB_00795"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR3341029.1"
                     /translation="MLSVTLRWAGGTLMVGTDSGGHSVTIGHSPDDPSQFIGMKPSDL
                     LLLAASSCSTYDVVNILKKQREPLQDIKVICSGEQLPDPPYTFTRIHIHYMVYGSVNP
                     EKLERAIHLAEDKYCSVISTLRCGTPVTSDYEIIEPEVSR"
     gene            complement(2632..3669)
                     /locus_tag="AC16CB_00800"
     CDS             complement(2632..3669)
                     /locus_tag="AC16CB_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013046.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIR3341030.1"
                     /translation="MQEEQPAMVFYYPEGHQAHFEPGHPERPERVERLCQAFRDAGWW
                     DAYPHMDSYAIPDEVLQAVHTPAYLDSLRDASRRGAYLDMDTYTTPASWQLAYNAAGG
                     AIAVASAVWGGRAQRGFALTRPPGHHATINQGMGFCLLNNISLAAEFLIQKEGAQRLA
                     IVDLDLHHGNGTQDIFWRRGDVFYLSTHQYPHYPGTGDLGEIGAGSGEGTTANFPLPP
                     GSGDQAFKGIVDELIIPLLERYSPEMILVSFGFDPHWRDPLGNLTLSALGYKDILSSL
                     VILAENVCQGKIALFLEGGYDLEAGAACGQAAVAALLNRPWKDPIGPSPQAESQYWLV
                     MAKRAKEIWNI"
BASE COUNT          995 a         1081 c          885 g          998 t
ORIGIN      
        1 taaggcgcgt gtcgcagccg cgcgcgcgtc gtcgatctcg accgtaaaga aagatgttga
       61 cggggccagt ccactggcaa tccaagccga gccaacgcca caaccggtgg caatctctcc
      121 gatgacaccg ctgagaaatg agctggtcaa tagctgcaaa agcctgcctg tctcgttgga
      181 gcatgagcct gaatactcca tttgctccgc cagattttga gcacgcctga cgagtggagg
      241 aatttgtgtc gtcgcccgat gatttaccat aaacttcctc cgtttccaaa ggctaattac
      301 tgaccacagt gcttcttgta ttctgggtta gctagctctt caatctcatc tactaaccgc
      361 gagaattcag ccaccaaagg agactcctca acctcagtga atacttctac caggctacga
      421 taataccaga gagtaccatc cctgccaccc ctaaatcgat caaacgcaga caatccgttg
      481 cgccgtagat cgctcaaaat cgagcgggca ttgtgcactt tatcagccag agaaacgatg
      541 cgcacatcat gcggttcccc ccgaagcttt gcaaggtacg cctcttttct ccccctccaa
      601 gggggcttag gctggagata tgagtccgtg cagccttcaa caatccgtgc tactcgttcc
      661 ccgaattgcc ggcggatttc agtcagggtt tccaagcccc cctggtcttc tacagcatcg
      721 tgcaacaggg cagcgatggc catctcttca tcacctcccc actcgatgac cagggctgtt
      781 accgcaagga ggtgcgctac atacgggata tcagtgcctt tgcggctctg accgttatgt
      841 agccgcgtgg catataccag cgcttgttca aaacgagctg tgaagtggtt tatttgcccg
      901 ctatctgtgt ccaatggagc ctctatatcg ggatcgtcag gctgtcaaga tctcgagggt
      961 aataggtcaa aacttcattg ccatcttcag taatcaggac cgtatcagaa tgcctgaaac
     1021 ctcccagctc cggtacatac aatcctggtt caacagtgaa aaccatgcct ggcttgataa
     1081 cagtcgcatc acctaagtcc aggaaaggtc cttcgtggta gcgcaatccg atggtatgac
     1141 ccacatgatg cttccaatag tccatcaagt cgtgagattc atagtatgcg cgcactgttt
     1201 tgtccaccgt cgaacaggat tggcccggaa ttaacgcctc gaaagcgata tcttgtaagg
     1261 cgaccatatg gtcgaacatg cgcttttgca tatcgctcgg agggcctaca accatagtcc
     1321 tctccagctc tgagttgtag ccccaaacgg gcgctcccgc cccggtcacc agcacatccc
     1381 caacttgaaa tgtgaggttg ttcgccagcg catgcgggat tgccgcgtta cgcccaattt
     1441 gccctcggta gttggcctct gcccccgtag agaaaacact ttgagcacgg tagatcggcc
     1501 caatcgtatc cagcatcgtc aatgttgctt ccatgctggc gcgcaggctg acctgggttt
     1561 cactctctcc cacgcgtgta tacctctgta ggagcacatg cgccagatgt ccccacttaa
     1621 cgctctcttt gatcagcgca atctcagccg ggcttttcac cgccatctga tcctcaatga
     1681 aacccgtcac gcggcgaggc ttccatccgg taagatctga taacgtcggc ccacggaacc
     1741 ccaaaatcca gggatatcca tcttgatctg ctccgtaatc ctctccaaaa ccgagccgtt
     1801 tcatcaattg ataaagtatt tccattggat gtggctcgaa aggatactca atgtagtatt
     1861 caacttggtc gacattggca tttgcctggg catgctccaa ttcaaggcgg gggacaaaca
     1921 gacacctttc ccccttggcg ttcattagaa atgccatcgg gcgctctgta gggacaaagg
     1981 caaatcccgt gtaatacagg atgtaataat tatcgaataa caccacttct gaaagatgtt
     2041 ctttctgaat ggtcgccagt agtgcatccg cgcgttgtcg gtattcctca tttgtgatat
     2101 gaattgatgt taatgccatt ctgtcctctt cacttccaga attcatctaa acaacaaata
     2161 tactgcagaa attatctacc ttacctcgaa acttctggtt caataatctc gtaatcactc
     2221 gtcacaggcg taccacagcg taacgtgctg attaccgagc aatatttatc ctcagctaaa
     2281 tgtatcgctc tttccagctt ttcaggattg acagagccat aaaccatata gtgaatatgg
     2341 atacgtgtga aagtgtaagg cggatcagga agttgctcgc ccgagcagat caccttgata
     2401 tcttgtagag gttcacgctg ttttttaagg atattaacca catcataggt tgagcaagaa
     2461 gacgccgcca agagcagcaa atctgaaggt ttcatcccaa taaattggga tggatcatct
     2521 ggcgagtgtc caatcgtgac tgaatgacca cctgaatcag tcccgaccat taaagtacca
     2581 cctgcccatc tcaaagttac agataacatg aatttttcct tctagttaat ctcagatatt
     2641 ccaaatttcc tttgcacgct ttgccatcac gagccaatat tggctctcag cctgcggaga
     2701 aggcccgatt ggatccttcc aagggcgatt gagcaatgcc gctacagctg cctgcccaca
     2761 agctgcacct gcttccaaat cataccctcc ctccaggaat aaagcgattt ttccctgaca
     2821 tacattctct gccaaaataa ccaggctcga gagaatatct ttgtatccca acgcagataa
     2881 ggtcagattt cccaggggat ctcgccagtg gggatcaaat ccaaaactga ccaatatcat
     2941 ttcaggtgaa tatcgctcta gcaaagggat gatcaattca tccacaatac ctttgaaggc
     3001 ttggtcacct gagccggggg gcaagggaaa gtttgctgtg gttccctcgc cggacccagc
     3061 cccaatttca cccagatcac cagttccagg gtagtgtgga tattggtgtg tggacagata
     3121 aaacacatcc cctcgtctcc agaaaatgtc ctgcgtccca ttgccatggt gcaggtcgag
     3181 atccacgatc gccaaccgtt gtgctccctc cttctgaatc aggaattctg cagccaaaga
     3241 aatgttgttc aacaggcaaa atcccattcc ctgattgatc gtggcatgat gacccggcgg
     3301 gcgggttaag gcgaagccgc gctgtgctct cccaccccat actgctgatg ccactgcaat
     3361 cgctccccca gcagcattat atgcaagctg ccaggaggcg ggtgtagtat aagtatccat
     3421 atcaaggtat gcgcctcgcc tggaggcatc tcttaatgaa tctaaatagg caggtgtatg
     3481 cacagcctgc agtacttcgt ctgggattgc ataagaatcc atgtggggat atgcatccca
     3541 ccaccccgcg tcccgaaatg cctgacagag tctctccaca cgttctggac gctccggatg
     3601 tcccggctcg aagtgcgctt gatgcccctc tggataataa aaaaccatcg cgggctgttc
     3661 ttcttgcata tcgccctcct cggaatcaat ttaatgcatg ggtagttgaa tgaatagagt
     3721 gggaatccaa tatcccaaaa tcattctcca tcaaatcctc agctgctaac ttctgtgatt
     3781 ataatttatc acgaacaaga catcgttggc acccacaaat aattcactca taattcagta
     3841 atccaatttt gttatatact tgatagtgag ataaggttat ccagatgagt atattattcg
     3901 tcgtaatcgg ttttatcgta cttgtaaccg ggcatcaagc tggttggata tttgccggg
//
LOCUS       DBNGSX010000026         6881 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1525301, whole genome shotgun sequence.
ACCESSION   DBNGSX010000026 DBNGSX010000000
VERSION     DBNGSX010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6881)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1525301"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="AC16CB_00805"
     CDS             complement(<1..188)
                     /locus_tag="AC16CB_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341031.1"
                     /translation="MTTNQSACIYCERDHNQVPLLQIQYQGNVYWICPQHLPVLIHKP
                     AQLAHKLPGAENLDPGVH"
     gene            complement(312..1733)
                     /locus_tag="AC16CB_00810"
     CDS             complement(312..1733)
                     /locus_tag="AC16CB_00810"
                     /inference="COORDINATES: protein motif:HMM:NF013828.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3341032.1"
                     /translation="MSKKIDETLKKLAASMQSGKTSAPDKTEAERNSSSSDLPGDPNC
                     PHCGGLGYLRQDLPPGHPDFGKLQVCSCRNSQVSQQVRQRLFALSNLDELTHLSFENF
                     QPRGRVGLWPQEADSLEQAHNQARQFAQSLNGWVVFQGGYGCGKTHLAAAIANFAVGM
                     GVPTLFITVPDLLDSLRYTYDNPQATFEERFEEIRRAPLLILDDFGTQNATAWSQEKL
                     FQIFNFRYINRLPLVVTTNTPLEDIEGRIRSRLEDPDLVTKVQILAPDFRRPAGDTGQ
                     NRVSSLDLLYKCTFANFDLREGEGLKPPDLKSLKEAFRVARRCAEHPQGWLVFSGPNG
                     CGKTHLAAAIGHHRRDLGDRVVYTVVPDLLDHLRATFNPQSTVRLDRRFEEVRTAPVL
                     ILDDLGTQAATPWVREKLYQLFNYRYNAELPTVITTADYVRDVDPRLLSRMEDRRLCE
                     ISAITAPAYRGAPQKPGRRTARR"
     gene            complement(1645..2361)
                     /locus_tag="AC16CB_00815"
     CDS             complement(1645..2361)
                     /locus_tag="AC16CB_00815"
                     /inference="COORDINATES: protein motif:HMM:NF018914.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HIR3341033.1"
                     /translation="MNSSFKGFPQGKVRLTPIPALFFHELLPYIDHLGELKVSLYAFW
                     RMSYQEGNFRYLQLGDFTGDERFMQGLGGTPEAAQAALHDSLERAVQRGTLLRASIEV
                     EEGEQSLYFVNSPRGREALQAIARGEWRPSGQAQQPVEILDEAPNIFRLYEENIGPLT
                     PMIADALREAEQEFPIQWLEDAMRIAVENNARNWRYVEAILERWQDKGRDEQKDRRDT
                     EKARRKYAEWENLGSGQDRS"
     gene            complement(2388..3767)
                     /gene="dnaB"
                     /locus_tag="AC16CB_00820"
     CDS             complement(2388..3767)
                     /gene="dnaB"
                     /locus_tag="AC16CB_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR3341034.1"
                     /translation="MNDRFFPEENPTPASPPVLYSREAEEAVIGSVLINPEAYYDVAS
                     FLKADDFHIHRHRWIWEVFVRLHERRIPIDYLTVSEELNQLGQLSEVGGPAYLTALIN
                     NVPTSLHAEAYGRLVEENAIRRKMLAAANDIAKLAYQESIGVEDVMDGAEKSIFGVSE
                     RRMTRDLQPIQQVLSEYYDRIDQLASRGEDIYGVPTGFIDLDRLLMGLQPSDFIIVAG
                     RPGMGKTAFMLSAAKNAAQTHKKHVAVFSLEMSNEQLVQRLIAQETGIDSQRLRTGKL
                     SEDEWPLFTHAIEVLSDARVFLDDTPALTPLQLRTKSRRLHLEYGLDLIVVDYLQLMS
                     SGTRSENRVQEVSYISRNLKVLARELNVPVLAAAQLSRAVEQRADKEPQLSDLRESGS
                     LEQDADIVMFIHRPEVYEKDTLKQNIAQIKVAKHRNGPVGMVELIFRHQLAKFENAAT
                     RVIDLTKVA"
     gene            complement(3936..4589)
                     /locus_tag="AC16CB_00825"
     CDS             complement(3936..4589)
                     /locus_tag="AC16CB_00825"
                     /inference="COORDINATES: protein motif:HMM:NF014618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HIR3341035.1"
                     /translation="MSDMVEVVIDSIRVSLMSQQRIVILREVNADRYLPIWIGVYEAE
                     AITIALQDVEVARPLTHDLLKNIFSALNAQIIRVEVSALKDDTFFGNIIAEVNGRNLN
                     VDARPSDALNLAVRAHVPILVARSVMDAAGITPEEDIQEQKQIESKTQGETPEPSEES
                     QERLSVFEDFLEKLELEDEDDENGKEDDNGGKGKKDDDSGKGKEDDDGGKGKKDKEE"
     gene            4977..5630
                     /locus_tag="AC16CB_00830"
     CDS             4977..5630
                     /locus_tag="AC16CB_00830"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3341036.1"
                     /translation="MVRVIWIEGKRAEGPSFVPNMRKKGYSVEVVSTGTEALERLVEI
                     DPDLAVVNAASMRTSGKRICRSLREKMNGLPIVLINSPEQPALGDPSANVVLTLPFTF
                     RKLSNRIVPLLPGNGGNMLHVGPIRLDMGLKRVRCQGREARLTPRLVRILRILMQHPG
                     EVVERAVLFRHVWDTEYTEDTRTLDVHISWLRQAIEEDPRKPQFLKTIRGVGYRLDI"
     gene            complement(5736..6077)
                     /locus_tag="AC16CB_00835"
     CDS             complement(5736..6077)
                     /locus_tag="AC16CB_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341037.1"
                     /translation="MPSELETALKNTATSVAKYVQDASELQVETRYVDVSSDGTPDFD
                     QARPVARTVIRLDGDSESVVPMRKGTGGALEVDATLFEVHQQNVAAAVDYRARILNAL
                     LSALPSRARTP"
     gene            complement(6091..>6881)
                     /locus_tag="AC16CB_00840"
     CDS             complement(6091..>6881)
                     /locus_tag="AC16CB_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341038.1"
                     /translation="NKQTMQNEAGFPPIFQNWQTAPVQSGDEISFDVATAPGEPASRW
                     SLELPAKPEEAKAALAQAEAQLQATQALLNEAPNRLDAFVSRLQSGTDAHFSIESFAV
                     EGVPGQPAEADLLPWIEYFEPGQISFDGQGITQGQAKEALDQLRQMIDGLVGQILYLA
                     QVETRQGGALLARSLVNWKGDLDTSWGEAISPEQHDLHQRSLALALASRIAMLRVIMT
                     TTQGAAKIAALIAAPGGALLALPAAWKYVSKILSEIEKYQTSSA"
BASE COUNT         1412 a         1841 c         1969 g         1659 t
ORIGIN      
        1 taatgtactc cagggtcaag attttcagca cccggaagct tgtgagccag ttgggcgggc
       61 ttatggatga gcacgggcag gtgttggggg caaatccaat agacattccc ctggtactgg
      121 atctgcaaga ggggcacctg gttatggtcg cgttcacagt aaatgcaagc ggattgattg
      181 gttgtcacat taaactccat tgtttacgat aaatttttct cgtagattca gcgaattatt
      241 ctcagcacaa atagaggatt agggcagaga ttcaatttgc tctcagtatc tcagtatttg
      301 gggtgaaatt gtcacctgcg cgctgtgcgt cgccccggct tctgtggcgc gccgcgatat
      361 gccggggcgg tgatggcgct aatctcgcac agtctacggt cttccatgcg gctgagcaag
      421 cgaggatcta catctctgac gtaatcggca gtggtgatca ccgtgggcag ctcggcatta
      481 tagcggtagt tgaacaactg gtatagtttt tctcgcaccc agggggtggc ggcttgcgtg
      541 cccaggtcgt ccagtatcag cacaggagcg gtgcgcactt cttcgaagcg gcgatccaaa
      601 cgcactgtgc tttgggggtt gaatgtcgct cgcaggtggt caagcaagtc gggcacaaca
      661 gtgtatacga cgcggtctcc gaggtctcta cggtgatgcc caatcgcagc cgccagatgg
      721 gtctttccgc agccatttgg tccagagaac accagccagc cctgggggtg ttcggcacag
      781 cgccgcgcga cccggaaggc ctccttcagg ctctttaagt cagggggttt caagccctcg
      841 ccctcgcgca ggtcgaaatt ggcaaaagtg catttgtata gcaaatctaa agatgagacc
      901 ctattctggc cggtatcccc agctggacgg cggaaatcag gggctagaat ttgcactttc
      961 gtgaccaggt ctggatcctc caggcgagag cggatgcgac cctcgatgtc ttccaggggg
     1021 gtgttggtgg tcacaaccag gggcaggcgg ttaatgtagc ggaaattgaa gatctggaag
     1081 agtttttcct gcgaccaggc ggtggcgttc tgggtgccaa agtcgtccag gatgagcaaa
     1141 ggggcgcggc ggatctcctc gaagcgctct tcgaaggttg cctgtggatt atcgtaggta
     1201 taacgcaggg agtccagcag gtcgggcacg gtgatgaaca gggtaggcac gcccatgcca
     1261 accgcgaagt tggcgatggc tgccgccagg tgggtcttgc cacagccgta gccaccctga
     1321 aaaaccaccc agccgttgag cgactgtgca aactggcgcg cctggttatg ggcctgctcc
     1381 agcgaatcgg cttcttgggg ccatagcccc acccggccgc gcggctggaa gttctcgaag
     1441 ctcaggtggg tcagctcgtc tagattgctg agggcgaaca ggcgctggcg cacctgctgg
     1501 ctcacttggc tgttacggca ggaacatacc tgcagtttgc cgaaatcggg atgtcctggg
     1561 ggcaagtcct ggcgcaaata accgagcccg ccgcagtgcg ggcagtttgg gtcgccaggc
     1621 aaatcgctcg aagaagagtt tctctcagct tctgtcttgt ccggagccga ggttttccca
     1681 ctctgcatac ttgcggcgag ctttttcagt gtctcgtcga tctttttgct catctctacc
     1741 tttatcttgc cagcgttcca ggatggcctc aacgtagcgc caattgcggg cattgttttc
     1801 tacagcaatg cgcatggcat cttcaagcca ttggatggga aattcttgtt cggcttcccg
     1861 cagggcatcg gcaatcatcg gcgtaagcgg cccgatattt tcttcgtata ggcgaaaaat
     1921 attgggggcc tcgtcgagaa tctcgaccgg ttgttgcgct tgcccggatg ggcgccattc
     1981 gccgcgtgca atggcttgca aggcttcccg tccgcgcggt gagttcacga agtacaggga
     2041 ctgctcaccc tcttctactt caatggatgc ccgcagcaac gtaccgcgct gcaccgcccg
     2101 ctccagcgag tcgtgcagcg ctgcctgcgc cgcttcgggt gtgcctccca gcccctgcat
     2161 gaaacgctcg tcccctgtga aatcgcccaa ttgcaggtag cggaaattcc cctcctggta
     2221 gctcatccgc cagaaggcgt acaggctcac ctttagctcg ccaaggtggt cgatatatgg
     2281 caggagctca tggaagaaga gtgctgggat aggtgtgagg cgtacttttc cctgtggaaa
     2341 gcctttaaaa gaagagttca ttgcacttgt attttacaac tataacgcta tgccacttta
     2401 gtgagatcga tgacccgtgt ggcagcattc tcaaattttg ctaattggtg gcggaagatc
     2461 agttccacca tgccaaccgg accattacgg tgcttggcaa ccttgatttg ggcgatgttt
     2521 tgcttgaggg tgtctttctc atacacctca ggccggtgaa taaacataac gatatccgcg
     2581 tcttgttcca gtgaccccga ttcgcgcagg tcggagagct gcggttcctt gtctgcgcgt
     2641 tgctccacgg cgcgtgatag ctgcgcggca gccagcacgg gtacattgag ctcgcgcgca
     2701 aggactttta gattgcgcga gatgtaagaa acctcttgga cgcggttctc ggagcgtgtg
     2761 ccgctcgaca ttaattgcag gtaatctact acgatcaggt ccagcccata ctccaggtgc
     2821 aagcggcggc tcttggtgcg taattgcaag ggggtgagtg cgggcgtatc atccaggaag
     2881 acgcgcgcat cgctcaacac ctcgatggcg tgtgtgaaca gcggccactc atcctcagag
     2941 agcttgccgg tgcgcaggcg ctgcgagtca atgccggtct cctgggcgat caggcgctgc
     3001 accagttgct cgttggacat ctctagcgag aacactgcca catgcttctt gtgggtctgg
     3061 gcggcatttt tggcagccga gagcataaaa gccgtcttgc ccatgcctgg gcgtccggcg
     3121 acaatgatga aatccgaggg ttgcaagccc atcaacaggc gatccagatc gatgaatcct
     3181 gtcggcacgc cgtagatgtc ctcgccacgg ctggcaagct ggtcaatccg gtcgtaatac
     3241 tcgctcaaca cctgctggat gggctgcaga tcgcgcgtca tgcggcgctc actcacgcca
     3301 aagatggatt tctcagctcc gtccatcaca tcttcgacgc cgatgctctc ctggtaggcg
     3361 agcttggcga tgtcattggc cgcggctaac attttgcggc gaattgcgtt ttcttccacc
     3421 aggcgtccat acgcctccgc atggagcgag gtgggcacgt tattgatgag tgccgtcaag
     3481 taagctggac cgcccacttc ggataattga cccagttggt tgagttcttc ggaaacggtt
     3541 agataatcaa ttgggatgcg ccgctcatgc aggcgtacga aaacctccca gatccagcgg
     3601 tgacggtgga tgtgaaaatc atcggctttc aggaaagagg cgacatcgta atacgcctcg
     3661 gggttaatca gcacggatcc tatgacggcc tcttcggctt cccggctata gagaacaggt
     3721 ggggaagcag gggtcgggtt ttcttcggga aagaatctat cgttcatcac atcctatcta
     3781 tacctgcata tcagattagg caaaaagcgc tgcgctgttc ctgcaaatcc ggcaatctga
     3841 gagggtggtc cggttttgac caaaaaaccg gggacaccag tttggctgcc tggcgaaccc
     3901 cggtgcacat ttgtcaatcg ggagtacgca gcgctctact cctccttatc ctttttcccc
     3961 ttgccaccat catcgtcctc tttccccttg ccactatcat cgtccttttt ccccttgcca
     4021 ccattgtcgt cctctttccc attttcgtca tcctcgtctt ccagttcaag tttttcgagg
     4081 aagtcctcaa ataccgacaa acgttcctgg ctttcctcag atggttctgg ggtctcgcct
     4141 tgcgtcttgc tttcaatttg tttctgctcc tggatgtctt cctcaggtgt gatgccagcg
     4201 gcatccatca cggagcgggc gaccaggatg gggacatggg ctcgcactgc caggttcagc
     4261 gcatcggaag ggcgggcgtc tacattcaga ttgcgcccgt tcacctcagc gatgatgttg
     4321 ccaaagaagg tgtcatcttt caaagcagac acctcgaccc ggatgatctg tgcgtttagg
     4381 gcactgaaga tatttttcaa taagtcatgg gttaatggtc gtgcaacctc gacatcttgc
     4441 agggcgatgg tgatcgcctc tgcctcgtaa actccgatcc aaatgggcaa atagcggtcc
     4501 gcgttgacct cgcgcagaat tacaatgcgt tgctgcgaca tcaggctaac ccggatgcta
     4561 tcaattacca cttcgaccat atctgacatg aatcactttt ccctagcttg gattgtatcg
     4621 tattctaaca cgggcagggc agggttgcaa ctatatttat caaaggccag ggcaattgca
     4681 tatgcccgga agggcatgcg caatcccgca aaaagagtgt ttggggtgtt ttggtggggg
     4741 tatgccccca ccaaaaacac cccattttct ctcctcagtg gtggggaatc gcccaaatgc
     4801 gattgccctg tatcaaaggc tctttaggat ttgtcttgac catggagatt ctcagttatc
     4861 cttaaaaaag cacatcggtt ctgattgtca gtttttaaga ttgcgagtat aatcaattta
     4921 acattttttt taacattggg gtttaaaaat tagcgattat accgataggg aagagaatgg
     4981 ttcgggttat ttggatcgag ggcaagcggg ctgaaggccc gtcttttgtc ccaaatatga
     5041 gaaaaaaagg ctattccgtg gaggttgttt ccacgggaac tgaggcgttg gaacgattgg
     5101 tggagatcga cccagacctt gcggttgtta acgcggcctc gatgcgcacc agcggaaaaa
     5161 ggatttgccg atctctgcga gaaaaaatga atggtctccc gattgtgctg atcaacagcc
     5221 cagagcagcc cgccctgggt gacccgagcg ccaatgtggt cctcaccctt ccgttcacct
     5281 tccgcaagct gtctaatcgc attgtgcctc tgttgcctgg aaatggcggg aatatgctcc
     5341 atgtggggcc tatccgcctg gatatgggac taaaacgcgt gcgatgtcag ggcagagaag
     5401 cccgtctcac cccccgcctg gtacggatcc tgcgcattct gatgcagcat cccggcgagg
     5461 tggtggagcg agctgtcttg ttccgccacg tatgggatac ggagtacacc gaggatacgc
     5521 gcactctgga cgtgcacatc agctggctgc gccaggcgat cgaagaagac cctcgcaaac
     5581 ctcagttctt gaaaaccatc cggggggttg ggtaccggct ggatatttaa tcagtttttg
     5641 gtgcgcaacg gcagtagtat aaccttatca accgcagtgt aactgcggtt gatgttgttt
     5701 ttccatattg tattattaca gcagctgaga tttgattatg gcgtgcgcgc ccgactgggc
     5761 agcgcactga gcagggcatt gaggatgcgc gcacgataat cgactgcagc ggcgacgttt
     5821 tgctggtgca cctcgaacag ggtagcgtcc acctctaatg ctccacctgt tcccttacgc
     5881 atggggacga ctgactcgct gtcgccatcc agccggatga ccgtgcgcgc caccgggcga
     5941 gcctgatcga agtcaggggt gccatcgctg ctgacatcga cataacgggt ctctacctgt
     6001 aattcagatg catcctgcac gtatttggct acgctggtag ctgtgttctt aagggcggtt
     6061 tcaagttcgc tgggcatttt tactcctaga ttatgcggat gaagtttgat atttttcgat
     6121 ttctgacaaa atcttgctca cgtatttcca ggcggctggc agggccagca aggcgccccc
     6181 gggcgcggcg atcagggccg cgattttagc agctccttgg gtggtggtca taatgactcg
     6241 cagcatggcg atgcgcgagg ccagagccag cgctaacgag cgctggtgca agtcgtgctg
     6301 ttcgggagag atggcctcgc cccaggaagt gtccaggtcg cccttccagt tcaccaggga
     6361 gcgagccagc agagccccac cctggcgcgt ctcgacctgc gccaggtaga gtatctggcc
     6421 tacgaggcca tctatcatct ggcggagctg atcgagagcc tccttcgcct gaccttgggt
     6481 tattccctgc ccgtcaaatg agatttgccc cggctcgaag tactcaatcc agggcaatag
     6541 gtcggcttcg gcgggttgcc ctggcacgcc ttcgacagcg aaggattcga tgctgaagtg
     6601 cgcatctgtg ccagactgca agcgcgagac gaacgcatcc agacgattgg gagcttcatt
     6661 taatagcgcc tgagttgcct gcagctgcgc ctcagcttga gccagtgctg ccttagcttc
     6721 ttccggtttg gcaggcagtt ccaggctcca ccgtgaggcc ggctcgccag gagcggttgc
     6781 gacgtcaaag gatatctcgt cgccactttg tactggggcg gtctgccagt tctggaaaat
     6841 gggtggaaaa cccgcttcgt tctgcatggt ttgtttattt a
//
LOCUS       DBNGSX010000027        20805 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1733002, whole genome shotgun sequence.
ACCESSION   DBNGSX010000027 DBNGSX010000000
VERSION     DBNGSX010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 20805)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20805)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20805
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1733002"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /gene="lipA"
                     /locus_tag="AC16CB_00845"
     CDS             complement(<1..898)
                     /gene="lipA"
                     /locus_tag="AC16CB_00845"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013988649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HIR3341039.1"
                     /translation="MPFTPTRDRVKPEDIDTTPRRRPEWIKVRAPSGETYEWLQGLMR
                     SKSLHTVCEEAMCPNLGECWGAGTATFLMMGDVCTRSCGFCDIKRGQPSPLDWLEPER
                     VAQAVKDMDLKHAVITSVNRDDRRDGGAPIFAMVIRRIRQLHPGCSIEVLIPDFKGSL
                     EALQIVMQARPEILNHNVETVPRLFKRVQPQDRYEWAAATLTNAKKLDPDVLTKSGIM
                     LGLGESIEEVKAVMRDQRSWGVDILTLGQYLQPSKKHLPIDRYYTLDEFAELKEFGLG
                     IGFQWVESGPLVRSSYHAAEQVR"
     gene            1238..2764
                     /locus_tag="AC16CB_00850"
     CDS             1238..2764
                     /locus_tag="AC16CB_00850"
                     /inference="COORDINATES: protein motif:HMM:NF027484.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HIR3341040.1"
                     /translation="MNRVQNACILLLLLLVILAAACAPVSLIPTAEPLESVYTAAAQT
                     VAVELTLEAGQTAVARLTEMAQQQPTPTIWLPTLPPPPPPTSTPPPPAPTSTVTPLPT
                     QPTCDTASFVADITVPPNATFSPGERFVKIWRVQNTGDCTWTSQYTLAFSGGEGMGSP
                     LLVSLPATVNAGQSVDVPIELVAPSTPGFHQGYWKLFDPNNAPIDIAPGPLGALWVQI
                     QVVDTVSTSGEYDFAREYCAAQWRSNRDVLTCSGFSDDPDGAVILLADPRLESRNEDE
                     LALWMIPGQGGGGWITGEYPPMRIYGGDRFVSEIGCLRDNPDCELLFELGYRTQDGNV
                     RTLDSWFETYDGDTREIDLDLEDLAGETVQFILSVTNQGDQDDANGFWLVPHIHNLSQ
                     RSDLVISWRQEGGTNEVCYDVKLYLTGRHDGEARARSCGGGIRESGTLRLNDAQVDLV
                     LDWLDTFRPYEFEVETPTNNEDLVEKIIFDGEGDIEVLYEDIRSMQNFMRSLYNAIIF
                     "
     gene            complement(2848..4167)
                     /locus_tag="AC16CB_00855"
     CDS             complement(2848..4167)
                     /locus_tag="AC16CB_00855"
                     /inference="COORDINATES: protein motif:HMM:NF051235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR3341041.1"
                     /translation="MTRRIWLLLCIVAGILVLWPWKATQAQGESPLVLVLTADGPLTP
                     AMLEYLERGIRTADDRGAELLVFQLNTPGGSIDLMQDMVEAIRSSPVPVAVYITPRGA
                     TAGSAGTVITLAGHVAAMAPETAIGAASPVGMEGQELGETIEAKAKNILKATVRSLAE
                     RRGPQAVDLAERTIEVADAASANEALEVGLVDFIADDLTGLLDQLDGFTVELITGERE
                     LNTANADIVPLSLSFIEQLLNMLTNPNIVFLLIAIGVQAVLIEISSPGGWVAGFIGVV
                     CLALATYGLGILPVNWFGIVFLILAFALFILDIKAPTHGALTAAGVGSLIVGALVLFN
                     SPSIPTFQRVSVPLVVGMSLITGATFFTILMIALRAQRSPIRIGQESLAGRRGVVRTR
                     LSPVGTVQVGGELWTAEPVEGEEALEPGTRIQVERVQGVRLYVRKIG"
     gene            complement(4320..5075)
                     /locus_tag="AC16CB_00860"
     CDS             complement(4320..5075)
                     /locus_tag="AC16CB_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HIR3341042.1"
                     /translation="MDAHIQIRNLNVYYGKTHALKDITLNIPKRQITVIMGPSGCGKT
                     TLLRCLNRFLELTDHARLEGQILIDGENPYAAGVDVTEIRKRTGLLAQRPHPLPMSIY
                     DNVAYGLRVHSLCKRGCDLDDRVRHYLEVAGLWDEVKNRLHAPASGLSLGQQQRLCLA
                     RGLAVEPEILLGDEPTSALDPISALHIEARLLELKQSYTTVIVTHILRQARRLADYVI
                     FMYLGEVVEHGPAHQLFEHPEKPRTRAYLEGQF"
     gene            complement(5091..5951)
                     /gene="pstA"
                     /locus_tag="AC16CB_00865"
     CDS             complement(5091..5951)
                     /gene="pstA"
                     /locus_tag="AC16CB_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR3341043.1"
                     /translation="MKISISRRRLEEGLVKTIMRASLLLVAGSLGLILATVIVKGLPS
                     LDLAMLTQTPKGGYYLGKEGGILNAIVGSLYLAGGATALALMLSLPIALYLQSYARRS
                     RWATLVRLGLDVLWGIPSIVYGAFGFSIMLALGMRASLLGGILTLALLELPIMTRAMD
                     EVIRMMPFDLEAASYAIGATRLETATRVVVRQMLPGILTALLLAFGRGIGDAASVLFT
                     AGYTDRIPMSLFRPVASLPLAVFFQLGTPFPEVQARAYSAALVLTVIVLGVSLCSRWL
                     AARLGRYVIR"
     gene            complement(5948..6973)
                     /gene="pstC"
                     /locus_tag="AC16CB_00870"
     CDS             complement(5948..6973)
                     /gene="pstC"
                     /locus_tag="AC16CB_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013667758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIR3341044.1"
                     /translation="MKTQPIRHVNGDAQPGANLLGALRWMEWPQLRLSRRLKDRLAAH
                     TFLAITTTPLILATTIIITLAWRAWPILAIVSPWELLFGRIWQPLKGAFGFYPFILGT
                     VWVTGIAMTLAVPPALLCAIYLAEYARPATRVVAKPVLDLLASIPSVVYGVWGVVAIV
                     PWVQGRVAPLLNRWLGYLPLFRTTNPTGFGLLSGGVVLAVMVAPFIIAVTYEVVCAVP
                     AGVREASLAIGATQWQTIRHVLLPHTLPGILASIVLGASRALGETMAVLMVVGNVAQV
                     PVSIFDAAYPLPALIANNYGEMLSIPLYDAALLGAAFILLLIVVIFNLASRLVLRHLT
                     GREVVKR"
     gene            complement(6976..7947)
                     /locus_tag="AC16CB_00875"
     CDS             complement(6976..7947)
                     /locus_tag="AC16CB_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR3341045.1"
                     /translation="MRAQISRRSLALILAIIAATLSGCASQSPASGGSGELDGTLTIS
                     GAWALYPMMTRWAEEFQILHPGVQFDISAGGAGKGMADALAGAVDIGMVSRDVSPEEE
                     AKGAFWVAVTKDAVFPMVSAENPVLGDLLSKGVNRQVFTGIFISGEITTWGQVIGQPE
                     MGESIHVYTRSDAAGAPETWAKFLGGKQEDLLGIGVFGDPGLLDAVIKDPLGIGYNNL
                     GYAYDNQSGLPVTGAIALPVDVDGDGQADPEEILETKAEAVEMVATGKYPSPPARALN
                     LVTNGKPSGLAQVFIAWILTDGQDFVGEAGYVHLTEAQLAAELEKVR"
     gene            complement(8046..8510)
                     /locus_tag="AC16CB_00880"
     CDS             complement(8046..8510)
                     /locus_tag="AC16CB_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR3341046.1"
                     /translation="MKLSKRGEYGVRAMIDLASWEGRNGMVQIKDIAEREHIPAKFLE
                     QILLTLKNAGLLQSKMGMGGGYYLAKPADQINLGHIVRVLDGPLAPIRCVSQMAYDSC
                     GCPDEHSCGLRMVMNDVRSAIADVLDRTTLADITKRVEMAHNMPGDTTRPND"
     gene            8729..9805
                     /locus_tag="AC16CB_00885"
     CDS             8729..9805
                     /locus_tag="AC16CB_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3341047.1"
                     /translation="MSQNPYQLLAQRLDSLPNGFPPTDDGVELRLLAELFTPQEAMLA
                     AQLRLSRETPEQIAARVGANPQLLREQLKSMARRGLIKAGRVEGGLGYGLMPFVVGIY
                     EMQVERLDAELAQLFEDYYHRAFGQMLSVRPSVHRVVPVGESVHMDIQIHPYESVTDI
                     LAAAQAWGVMDCICRKQKALIGDPCQHPLDVCMILSQKPGAFDQSSFVRALTYDQAQE
                     TLGRAAQAGLVHSVSNSQSGIGYICNCCTCSCGILRGIAELGFANAVAHSAFINQVDE
                     DLCVGCELCVEYCQFDALTMDNIARVDAIRCVGCGVCVPSCPDGALGLVRRPSQEIET
                     TPVDEIAWLQERAAARGLDLGSVL"
     gene            9802..11001
                     /locus_tag="AC16CB_00890"
     CDS             9802..11001
                     /locus_tag="AC16CB_00890"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008235 - metalloexopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HIR3341048.1"
                     /translation="MIASSFREDVLRALEHIRYLSVIIGGRGSCTAAERQAAQYTVDQ
                     MDEMGVKNAHLEFYNGAASTYRPYALAFGAALLGTLLAWLGEGRLWLALAALLNVLGV
                     LGMLAETDFASNWTRWFSPRASSQNAVGIIPASIQLRRRAVLCAHVDSHRTPIFYSSP
                     TWRKLFQLLVSTAYLNMAVGGAFFALGALLGITWVRWFGLIIAVIQVSAILLCLHADF
                     TPFSPGANDNASGVGVILEIARRLVKQPLDFTEVWLAFTGCEETGASGMSAFLDSHAE
                     ALGSDTVYIIVDEAGLGKLQYLTNDGLVIKRNTHPQALELANRVGVLLPEPRATPRGG
                     IAYTDAAVATKRKLIALSLGCLPEAQAGQSSHWHQLSDTIDTIDRQALEDSLLFTWHL
                     LRQVDNY"
     gene            11324..12229
                     /locus_tag="AC16CB_00895"
     CDS             11324..12229
                     /locus_tag="AC16CB_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HIR3341049.1"
                     /translation="MKRAVSISIGSSKRDKAVEVELLGEKVRIERIGTDGDMEKAAQL
                     YKELDGKVDAFGVGGADLGVLVDDKWYPLYSVQSMVRFVEQTPLVDGAGLKNTLENRL
                     GPFIDSKIGDYVQALGKKAFITLGVDRWGMSCSFIDAGYECVFGDLMFGLGLPFTIRS
                     AQALKRLAGILMPIAGRLPFEWIYPTGEKQEERKPKWESYYNWGTVIAGDCHYIKRHM
                     PERLDGKVIATNTTTVSDVEAFRKAGVKYLVTSTPVLEGRSFGTNMMEAALVAVSGKG
                     RELKRDELNQLLDQLGFQPELQELN"
     gene            12231..13022
                     /locus_tag="AC16CB_00900"
     CDS             12231..13022
                     /locus_tag="AC16CB_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HIR3341050.1"
                     /translation="MDALVTAGGIPQPGEPLYDYTQGISKALLEIAGKPMIQWVFDAL
                     DGAASIQRVVTIGLDSESNVTCHKLIGFLPNQGGMVDNIRAGIFKLIELDPEPRPVLV
                     VSSDIPGITSEMVEWAVSTAMQSNDDVYYNVIPRQVMEKTFPESKRTYTRLKDLEVCG
                     GDMNIVRSSLVTSKDEIWEKLIASRKNVLKQAALIGYDTLLLLLMRGITLQDAVQRVA
                     RRLHITGRAVVCPYAEVGMDVDKPHQLELIRAFLERRIVQGASAS"
     gene            13019..13549
                     /locus_tag="AC16CB_00905"
     CDS             13019..13549
                     /locus_tag="AC16CB_00905"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR3341051.1"
                     /translation="MSWQKSLIELDERITSWLRIAERPGLLRNLAILLAHSGDSWFWL
                     LGLVLLWWLGGDFWQGRALELGVGILVTAVLVMLIKFSVRRRRPVGEWGKIYRNTDPH
                     SFPSGHAARAILLAVMAVGLGPGWFALLLWFWAPLVVLARVAMGLHYFSDVLAGAGLG
                     FVMGIVILRFLPAFPF"
     gene            complement(13515..14582)
                     /locus_tag="AC16CB_00910"
     CDS             complement(13515..14582)
                     /locus_tag="AC16CB_00910"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HIR3341052.1"
                     /translation="MNLDDLAYFSKVDIQHMHDQINSLPDQLEKAWSLGSSQSLPNWQ
                     GIRQVVIAGMGGSAIGGDLLSAYVEPFCKVPVIVHRDYGLPAWGRGPETLVIASSHSG
                     NTEETLSSFKEAQANNCRILAISTGGKLAEAAKNAGTPLWVFEHHGQPRAAVGYSFGL
                     LLSALAHLDLVPDHSDEIMDAAAAMKAQQAQLVASTPVVKNPAKRIAGQLMGRWVTVF
                     GAGILAPVARRWKGQVNELAKAWGQYDLLPEADHNTLAGILNPPEQLPRMVALFLRSP
                     SDHPRHRLRLDLTRKSFMLEGISTDFIDATGDSRLANQWTGLHFGDYTAYYLAMAYGI
                     DPTPVDPIETFKREMQAESAK"
     gene            complement(14588..15424)
                     /gene="rsmI"
                     /locus_tag="AC16CB_00915"
     CDS             complement(14588..15424)
                     /gene="rsmI"
                     /locus_tag="AC16CB_00915"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006516138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR3341053.1"
                     /translation="MGILYLVATPIGNLEDISARALRILGEARLIAAEDTRHTRKLLS
                     HFGIHTPMISYHEHNKLTRLDEVLAALAQGDVALVSDAGTPALNDPGYELVGAAIAAG
                     YTVSPIPGPTAPIAALVASGLPTDAFLYLGYLPRKAAERRNLLAQVASMPFTLIFLET
                     PHRLMGALEDMQSKLGDRPCAIARELTKLHEEIYRGSLGEALRHFGAQKPRGEFTIVV
                     AGSSIQQEPRWTEEKLRSALQSGLDQGESPSNLAKRIASKSGWSRREIYRMIANKHER
                     EE"
     gene            complement(15424..16494)
                     /locus_tag="AC16CB_00920"
     CDS             complement(15424..16494)
                     /locus_tag="AC16CB_00920"
                     /inference="COORDINATES: protein motif:HMM:NF027484.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HIR3341054.1"
                     /translation="MKLSLIRSSLLAFLILAIVSGCNIPRREASSTDINVTQAYETVQ
                     ARLTQNSQSNTSSQGTPTPTTGLTSPTPLTTPPTITPTREPTANQPTTTSRFSCDQAS
                     PGNPIDVTIPDDTPLQPGQSFTKVWRLQNTGTCTWNNTYAVAFFSGDQMGAPASVALA
                     GNVAPGQSVDISVDMVAPSKAGNYQGNWKLRNASNVLFGIGPGGAAPFWVRIVVSVTS
                     TPSSTPRTPTPTSTPTATATATSPVHIVGTANLNVGATLDLDNDQINGGGADIIYEPI
                     DSKHQFAPQDSTLLGVYGMSQPGQANCQSSSLDTSPIVVEDIPLNSYICYRTHEGRYG
                     RARLSEFNAVNFTVRLEFLTWP"
     gene            complement(16513..17664)
                     /locus_tag="AC16CB_00925"
     CDS             complement(16513..17664)
                     /locus_tag="AC16CB_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009247 - glycolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGDG synthase family glycosyltransferase"
                     /protein_id="HIR3341055.1"
                     /translation="MKHPVPGKPHILILFSDTGGGHRSAAEAIIEALHLEYEEQFTIE
                     MVDIFKDIALPPLNRMPDLYPTMVRIPQAWGFGFRLTNGSQRSRLLTSSTYPYVRKAL
                     RKLIAKHPSDLIVSVHPLANAPILRSLGKDRPPYITVVTDLVTAHALWYHRLTDLCLV
                     PTEAARQRALRYGLKPDQVKVVGLPVANRFCIAAGDPVELRNQYGWRQDRPVILLVGG
                     GEGMGPLEKTARAIADAGLNASLVVITGRNESLKARLESMSWSIPTHIYGFVREMPNF
                     MRAADILVTKAGPGTICEALNAGLPMILYSRLPGQEEGNVSYIVSEGAGVWAPQPEKI
                     VATLHNWITHPQELAQAEAACRRLARPQAARQVAHILAGFVGIQYQPGA"
     gene            complement(17642..17863)
                     /locus_tag="AC16CB_00930"
     CDS             complement(17642..17863)
                     /locus_tag="AC16CB_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HIR3341056.1"
                     /translation="MRLMLPDIQLDDVVRLRKPHPCGSYEWRIVRLGADIGLVCLGCG
                     RRVLLDRRTLAKRMKKILPKTSDETSGAG"
     gene            complement(17939..20716)
                     /locus_tag="AC16CB_00935"
     CDS             complement(17939..20716)
                     /locus_tag="AC16CB_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009547575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR3341057.1"
                     /translation="MGTWYQQDTEEVLNILDTSAMDGLSDEIATNRLEQYGPNELEER
                     GVKSPWLILWEQLTETMVVILIISAVISSILGDLKDAVAILAIVVINTLLGFRQEYQA
                     EKAMAALKKLAVPVVKVRRGGHVLEISSRDLVPGDIVLLEAGNLVPADCRILESVNMR
                     TQEAALTGESEPVSKSSQAQPGKSLPLAERYNMVYMGTLVTYGRGIGVVAETGMRTEL
                     GNIAELIQSAAQEPTPLQQRLNRLGKQLAWVALGLVILVFLLGVARGEELRLLFLTAI
                     SLAVAAVPEGLPAVVTISLALGARRMLRRQALIRKLPAVEALGSVTVICSDKTGTLTE
                     NRMTVTVIDLAEHRIDLSEEETRGYFSENLEPSHSETPGHQQLAYLNENPALTVMLAG
                     SVLCNDAYLEYDENTSQRFHIVGDPTEGALMVAAARMGLRREALEAIMPRVAEAPFDS
                     DRKRMTTLHELKSEQLEGWQETLLELLNLQPENLRLIAFTKGAMDGLLEVSDRVWVQN
                     HIEPLDAGWRKRIEDANERLAMEGVRVLGVASRAVDDQPEGDDVFKLEQGLIFIGLVG
                     MIDPARAEVKQAVQTCITAGIRPIMITGDHPLTARYIARELEIDIENGVPTGNDLDRM
                     SAQELEETVGKVSVFARVSPEHKLKIVQALQNRGQIAAMTGDGVNDAPALRQADIGVA
                     MGITGTDVSKEASDMVLLDDNFATIVAAVEEGRVIYDNIRKFIKYTFTSNAGEIWVMI
                     LAPFLGMPLALLPLQILWINLVTDGLPGLALGIEKAERNTMRRPPYPPNENIFARGVG
                     RDILFIGALMGLLSLGVGFWYWRMGHPTWQTMVFTTLTLSEMGYVLAIRSNRDSLFTI
                     GVFSNWALIGAVILTTLLQIAVVYVPFLQSIFETMALPVRDLGIAMLLSTSLFIVVEL
                     QKQLMRRLSPK"
BASE COUNT         4448 a         5840 c         5981 g         4536 t
ORIGIN      
        1 cacgcacctg ttcagctgcg tggtaggatg agcgcaccag cgggccgctc tccacccact
       61 ggaacccgat gcccaggccg aactccttga gctcggcgaa ctcgtccagg gtgtagtagc
      121 ggtcgatagg caggtgtttc ttgctgggct gcaggtactg gccgagggtc aaaatgtcca
      181 ccccccagct gcgttggtcg cgcatcactg ctttgacttc ttctatgctc tcgcccaacc
      241 cgagcatgat gcccgacttg gtgagcacgt ccgggtcgag ctttttggcg ttagtcagcg
      301 tggcggcagc ccattcgtag cggtcttgcg gctgcacccg cttgaacagg cgcggcacgg
      361 tctccacatt gtggttgagt atctcagggc gggcctgcat gacgatctgc agggcttcca
      421 ggcttccttt aaaatcgggg atgagcactt caatggagca gccggggtgc agctggcgga
      481 tgcggcggat gaccatggcg aagatcggcg cgccgccgtc tctacggtca tcgcggttca
      541 cgctggtgat cacggcgtgc ttcaggtcca tatccttcac cgcctgggcc actcgctccg
      601 gctccagcca gtctaaggga gacggctggc cgcgcttgat gtcgcaaaag ccgcacgagc
      661 gcgtgcacac atcgcccatc atcaggaagg tggcggtgcc cgcgccccag cactcgccca
      721 ggttggggca catggcctcc tcacacaccg tgtgcagcga cttgctgcgc atcagccctt
      781 gcagccactc gtaggtctcg ccagacgggg cgcgcacctt gatccactcc gggcggcggc
      841 ggggtgtggt atcgatgtct tcgggtttca cccggtcgcg ggtgggggta aacggcatac
      901 aagctcctcg tgtggcgcca ggcgtggcgg caccctgatt gtaccctgat ttatgcgcct
      961 taccttccta ttttaaccca catctcatct ggagttgccc ccccacgcgc gacctgccag
     1021 aaaacatccc gcacctgttg atgataaacc tgcgggacgt tcatcaccaa gatacccgca
     1081 tgcccataag gacgccacct cataaaagcc atcaacccat ggccagggca attgcatata
     1141 ccctgagggg catgcgcaat ctcgcaaaaa cgaggctata ataggggcgc cagccagtac
     1201 ctcaggcacg ctcaagggat ccggtatggg acgagagatg aacagggtac aaaatgcatg
     1261 catcctattg ctcctgctct tggtaatcct tgccgctgct tgcgcgccag tgagcctgat
     1321 ccccaccgcc gagcccctgg aatccgtcta taccgccgcc gcccagaccg tagccgtgga
     1381 gctgaccctg gaggccggcc agacagccgt cgcccgcctg accgagatgg cccagcagca
     1441 acccacgccg accatctggc tgcccacgct cccacccccg ccaccgccca ccagcacccc
     1501 gccaccgcct gccccgacct ctaccgtaac ccccctaccc acccagccta cctgtgatac
     1561 cgccagcttt gtagccgata tcacggttcc gccgaacgct accttttcac ctggagagcg
     1621 cttcgttaag atctggcgcg tccagaatac aggcgactgc acctggacgt cgcaatacac
     1681 gctggccttt tctggcggcg aaggcatggg cagcccgtta ctggtcagtt tgcctgccac
     1741 ggtcaacgcc ggtcaatcgg tggatgtgcc cattgagctg gttgccccat ccacgccggg
     1801 tttccatcaa ggctactgga agctgttcga cccaaacaac gccccgatcg atattgcccc
     1861 tggcccgtta ggtgctttgt gggtgcagat ccaggtggtg gatacggttt caaccagcgg
     1921 cgaatatgac ttcgccaggg agtactgcgc cgcccaatgg cgcagcaacc gggatgtgct
     1981 cacctgttcg ggcttcagcg atgaccccga tggagccgtg attctcctgg ccgacccgcg
     2041 cctggaatcg cgcaacgaag acgagctggc cttgtggatg atcccgggtc agggtggggg
     2101 tggctggata accggcgagt acccgccgat gcgcatctat ggcggcgaca ggttcgtctc
     2161 ggagatcggc tgtctcaggg acaacccgga ttgcgagctg ctcttcgagc tgggctaccg
     2221 cacccaagat ggcaatgtca ggacactgga ctcctggttt gaaacctacg atggcgacac
     2281 ccgcgagatt gacctggacc tggaagacct ggcaggcgag acggtgcaat ttatcctcag
     2341 cgtaaccaac cagggagacc aggatgacgc caacggattc tggcttgtgc cgcacatcca
     2401 taatctctcc cagcgttcag atctggtcat cagctggcga caagaagggg gcaccaatga
     2461 ggtttgttac gatgtcaagc tgtacctgac cgggcgccat gacggcgagg cgcgcgcccg
     2521 cagctgtggg ggcggcatac gagaatcggg caccctgcgc ttgaatgacg cccaggttga
     2581 cctggtctta gactggttgg acacattcag gccctacgag tttgaggtcg agacgcctac
     2641 caacaacgaa gatttggtcg agaagatcat cttcgatggc gagggggata tcgaagtgct
     2701 ctacgaagat atccgcagca tgcagaattt catgcggagc ctgtacaacg ccatcatctt
     2761 ctaaccgccg taccgtagat tagaatgctt ttgacaatct cccacccctg gaccactggg
     2821 gtagagcatg actaacggaa aaacgggcta gccgatcttg cgcacgtaca gccgcacccc
     2881 ctgcacgcgc tcgacctgga tgcgcgttcc aggttccagg gcttcttctc cctcgacggg
     2941 ctccgccgtc cacagctcgc cgcccacttg cacggtaccc accggggaca ggcgggtgcg
     3001 caccacgccc ctgcgcccag ccaggctttc ctgcccgatg cggatagggc tgcgctgggc
     3061 gcgcagggcg atcatcagta tcgtgaagaa ggttgccccg gtaatgagcg acatgcccac
     3121 caccagcggc accgacactc tttggaaggt ggggatgctg ggcgagttga acaacaccag
     3181 cgcgccgact atcagcgagc ccacgccggc ggcggtcaat gccccgtgtg tgggcgcttt
     3241 aatatccagg atgaagagcg caaaagccag gatgagaaac acgatcccaa accagttgac
     3301 gggcaatatg cccagtccgt aggtcgccag cgccaggcac accacgccga tgaagcccgc
     3361 cacccacccc cccgggctgg agatttcaat caagactgcc tgcacgccga tggcgatcag
     3421 caggaagacg atattcgggt tggtgagcat gttgagcagc tgctcgataa aggagagcga
     3481 gaggggcacg atgtcagcat tggcggtgtt cagctcccgc tcgccggtga tcaattccac
     3541 cgtgaaccca tccagctgat ccagcaggcc ggtcaaatca tcggcgatga aatccaccag
     3601 accaacctcc agggcttcat tggcagaagc cgcatccgcc acctcaatcg tccgctccgc
     3661 caaatcaacc gcctgcgggc cgcgccgctc agccagggag cgcaccgtgg ctttgaggat
     3721 gttcttggct ttggcttcga tggtctcgcc cagctcctgc ccttccatgc caaccgggct
     3781 ggctgctccg atagccgttt cgggcgccat ggcagccaca tgccccgcca gggtgatcac
     3841 tgtgccagcg ctgcccgccg tggcgccgcg cggtgtgatg tacactgcca cgggcacagg
     3901 gctggagcgg atggcttcga ccatatcctg catcaggtca atgctgccac ccggggtgtt
     3961 cagctggaaa acgagcagct cagccccacg gtcatcagcc gtgcgaatgc cgcgctccag
     4021 gtattccagc atagccggtg tgagcgggcc atcggcggtc agcaccaaaa ccagaggcga
     4081 ttcgccctga gcctgggttg ccttccaggg ccataatacg agtattcccg caacgatgca
     4141 aagcagtagc catattcgcc tggtcataat caactccacg attgagtgga accgctcaat
     4201 ccataactca attatagcaa atctggcagg cctggatacc cggctcagaa ttgccccagg
     4261 gcaagcgcaa tcccgtaaag agagtgattg gtgtgggaaa tcgtcctaat gcgattgccc
     4321 tagaattgcc cttccaggta ggctcgcgtg cgcggcttct cggggtgctc gaaaagttgg
     4381 tgagccgggc cgtgttccac cacttcgcca aggtacatga agatcacgta atcggcgagg
     4441 cggcgagcct ggcgcaggat atgtgtcacg atcacggtgg tgtaagactg cttcagctcc
     4501 agcaagcgtg cttcgatgtg taaggccgag atcggatcca gcgccgaggt cggctcatcc
     4561 cccaacaaga tctcaggctc gaccgccagg ccgcgcgcca ggcataatcg ctgctgctgc
     4621 ccaaggctca aaccagatgc cggggcatgc aggcggtttt tcacctcgtc ccacaagcct
     4681 gccacttcca ggtagtggcg cacgcggtcg tccaggtcgc agcccctctt gcacaaggaa
     4741 tgcactcgca gaccgtaggc gacgttatcg taaatagaca tcggcagcgg atgcgggcgt
     4801 tgagccagca agccggtgcg cttgcggatt tcggtcacgt ccacccctgc ggcataggga
     4861 ttctccccat cgatgaggat ctgcccctcc aggcgggcgt gatcggtaag ctccaggaag
     4921 cggttgaggc accggagcag ggtggttttt ccgcaacccg aagggcccat gatcaccgtg
     4981 atctgccgtt tcgggatatt cagggtgata tccttcaggg cgtgcgtctt tccataatat
     5041 acattcaaat tgcgaatttg gatatgcgcg tccattggca acctccgaga tcagcggatg
     5101 acgtacctgc ccagcctggc agccagccag cgcgagcaca ggctgacgcc cagcacgatt
     5161 accgtcagca ccagcgcggc agaatatgcc cgcgcctgca cttctgggaa gggggtgcct
     5221 agctggaaga ataccgccag cggcaaggat gccacagggc ggaataaaga catggggata
     5281 cgatccgtat aacctgcggt gaagagcaca gaagccgcat cgccaatccc ccgcccaaag
     5341 gcaagcagca gcgcggtgag gatgcccggc agcatctgcc gcaccaccac gcgcgtagct
     5401 gtctccaaac gggtggctcc aatagcatag gaagcagcct ccaggtcaaa aggcatcatg
     5461 cgtatcacct cgtccatggc acgggtcatg atgggcagct ccagcagcgc cagcgtcaag
     5521 atgcccccca ggagcgaggc ccgcatcccg agggccagca tgatgctgaa tccaaaggct
     5581 ccatatacaa tggatgggat gccccacagg acatccagac ccaggcgcac cagggtagcc
     5641 cagcgcgagc ggcgggcata cgactgcagg tatagggcga tgggcaggct gagcatcagg
     5701 gctagcgcag ttgctccgcc agccagatac agcgatccaa cgatggcgtt caaaatgccg
     5761 ccctccttgc ccaggtagta gccgccttta ggtgtctggg tgagcatagc gagatccagg
     5821 ctaggcagcc ccttgacgat cacggtcgcc aggatcaatc ccaggctgcc tgcaaccagc
     5881 agcaaggaag cccgcataat ggttttgaca agcccttctt ccaggcggcg tcgggaaata
     5941 gaaattttca tcgcttcaca acctcccttc cggtgaggtg gcgcagcacc agcctggatg
     6001 ccaggttgaa aatcaccaca atcagcagca agataaaggc tgccccaagc aaggccgcat
     6061 cataaagcgg gatggaaagc atctcaccgt agttattcgc aatcaaagcc ggtagcgggt
     6121 aagcagcatc gaagatggat acaggcacct gggccacatt gcccaccacc atcagcactg
     6181 ccatagtctc gcccagggcg cgggaggctc ccagtactat gcttgccagg atgccaggta
     6241 aggtgtgggg cagcaggacg tggcggatag tctgccactg tgtagcgccg atagccagcg
     6301 aggcctcgcg aaccccagcc ggtaccgcgc aaacgacctc gtaggtgacc gcgatgataa
     6361 acggcgctac catgaccgcc agcacaacac cccccgagag caagccgaag cccgtcgggt
     6421 tagtagtacg aaaaagtggc aagtaaccca gccagcgatt cagcagcggg gcaactctcc
     6481 cttgcaccca ggggacgatg gctaccacgc cccacacgcc atataccacc gaagggattg
     6541 aggccagcaa gtcaagcaca ggcttagcga ccacccgtgt agccgggcgg gcatattctg
     6601 ccaggtagat ggcgcacagc aaggccggtg gcacagccag tgtcatcgcg atcccggtta
     6661 cccaaaccgt cccaaggatg aatggataga agccaaatgc tcccttgaga ggctgccaga
     6721 tgcgcccgaa cagcaattcc caaggggaga cgatggctaa gatcggccag gcgcgccagg
     6781 ccagcgtgat gatgatggtt gtcgccaaga tcaatggggt ggtagtgatc gccaagaagg
     6841 tgtgcgcggc gaggcgatcc tttagccggc gagacagacg cagttgtggc cactccatcc
     6901 agcgcagggc acccagcagg ttcgcgccgg gctgcgcatc gccatttacg tggcggatcg
     6961 gttgagtctt catctttatc tcactttttc cagctcagcg gctagctgtg cttcggtcag
     7021 gtgcacataa cccgcctcgc caacaaagtc ctgcccatcg gtcagaatcc aagctataaa
     7081 aacctgcgcc aggccggacg gttttccatt cgtgaccagg ttgagggcgc gcgccggcgg
     7141 agacgggtac ttgcccgtgg cgaccatctc aaccgcctcg gctttggtct ccaggatttc
     7201 ttcgggatct gcctgcccat ctccgtccac atccaccgga agggcgatcg cccccgttac
     7261 cggcaagccg ctctggttat cataggcgta gcccaggttg ttatagccga tccccagggg
     7321 gtccttgatg accgcgtcca gcaggcccgg atccccaaac accccaatcc ccagcaggtc
     7381 ttcttgcttg ccccccaaga atttcgccca tgtctcgggg gcgccggccg cgtcagagcg
     7441 tgtgtacaca tggatagact cgcccatctc cggctgccca atcacttgcc cccaggtagt
     7501 gatctctccg ctgataaaga tgccggtgaa aacctggcgg ttgacgcctt tgctcaatag
     7561 gtctcccaag acaggatttt cggcgctcac catagggaat acagcatcct ttgtcacagc
     7621 cacccagaaa gcgcctttgg cctcttcttc aggggaaaca tcacgagaga ccataccaat
     7681 atccaccgcg ccagccaggg cgtcggccat gcctttaccc gccccgcccg cggagatatc
     7741 aaattgcacg cccgggtgca gtatttgaaa ttcttctgcc caacgggtca tcatgggata
     7801 gagtgcccag gcacccgata tggtcagggt gccatccaat tcgccagatc caccgctagc
     7861 cggtgattgg ctagcgcatc cgctcaacgt cgccgcaatg attgccagga ttagcgccag
     7921 agatcgtctt gaaatttgag ctcgcatggc attctccttt atgtctagta agtctagtct
     7981 tctaatagac tttttggcaa taagaaaaca acctcgcgcc tttcgggttt tccagtaaag
     8041 ttcactcagt catttggccg cgttgtgtcc ccaggcatgt tgtgtgccat ttccaccctt
     8101 tttgtgatgt cggctaacgt ggtgcggtca agcacgtctg cgatggcaga gcgcacatcg
     8161 ttcatgacca tgcgcaagcc gcaagaatgt tcgtcagggc agccgcatga atcataggcc
     8221 atctggctca cacagcggat gggggccagc gggccatcga gaacgcgcac aatatgaccc
     8281 aggttgatct ggtcggcagg tttagccagg tagtacccgc ctcccatccc catcttgctt
     8341 tgcagaaggc ctgcgttctt gagtgtcagc aagatctgct ccaggaattt cgccgggata
     8401 tgctctcgct cggcgatatc cttgatctgc accatcccat tacggccctc ccagctggcc
     8461 aagtcaatca tggctcgcac accgtattca ccacgtttgg atagtttcat agattaggct
     8521 cgtatattgt aggggaacaa tatcctacaa agtaaagtgt ttctatagta tttattacat
     8581 tcttagaata tacgcgaatt tgtcttttgt caattggatt tttgtcactt gtcttgctgg
     8641 catttttctt gtattctatt cgggtggcat aacgtctctt gcttagatgc ttgcacgacg
     8701 atgcccgaaa acccgaagga ggtagataat gagccaaaat ccctatcaac tgctggctca
     8761 acgcctggat agcttgccca atggctttcc accaactgat gatggcgtcg agctgcgcct
     8821 gcttgccgag ctattcaccc cgcaggaggc catgctcgct gctcagctac gcctgagccg
     8881 tgagactccc gaacagattg ctgcccgcgt cggagccaac ccgcagttgc tgcgcgagca
     8941 gcttaaatcg atggcacggc gcgggttgat caaagccggg cgggttgaag ggggtcttgg
     9001 ttacggtctg atgcccttcg tggtgggtat ttacgagatg caggtggagc gcttggacgc
     9061 tgaactagcc caactctttg aagattacta ccaccgggct ttcgggcaga tgcttagcgt
     9121 gcgtccatct gtacaccgcg tggtaccagt gggagagagc gtgcatatgg atatccaaat
     9181 acacccctat gagagcgtga cagacatcct ggctgctgcc caagcctggg gagtgatgga
     9241 ttgcatctgt cgcaagcaga aagccttgat tggcgatcca tgccagcacc cgctggatgt
     9301 ttgcatgata ctgagccaga agccaggcgc cttcgaccaa agctcctttg tgcgggcatt
     9361 aacctacgat caagcccaag agacgctggg gcgggctgcg caggctggtc tggtgcactc
     9421 ggtgagcaat agccagagtg gtattggtta tatctgcaat tgctgcacct gttcttgtgg
     9481 catcctgcgc ggtattgccg aactggggtt tgccaacgcg gtggcacact cagccttcat
     9541 aaaccaggtg gacgaagacc tgtgtgttgg ttgtgagcta tgtgttgagt attgccagtt
     9601 tgacgccttg acgatggaca acatcgcacg cgtggatgca atccgctgtg tgggctgtgg
     9661 cgtgtgcgtg ccatcctgcc cggatggcgc cctgggattg gtccgccgcc cgagccagga
     9721 aatcgagacc acgccagtcg atgagattgc ctggttacaa gagcgcgccg ctgcccgcgg
     9781 cctggacctg ggaagcgtac tttgatcgca tccagctttc gagaagatgt tcttcgagcg
     9841 ctggagcaca tccgttactt atcggtaatc attggcggaa gggggagctg caccgcagcc
     9901 gagcggcagg cggcacaata taccgtggat caaatggatg agatgggcgt gaaaaacgcc
     9961 caccttgaat tttataacgg ggcagcctct acctaccggc catacgccct ggcatttggc
    10021 gccgcactcc tgggcacctt gcttgcctgg ctaggcgagg gtcgtttgtg gttggcactc
    10081 gcggctttgc tgaatgtctt gggtgttctg ggcatgctgg cagagactga cttcgcctcg
    10141 aattggacgc gctggttctc cccgcgcgcc agcagccaaa atgctgtggg gatcatccca
    10201 gcctcaatcc agctacgtcg gcgcgcggtg ctgtgtgccc atgtggacag ccaccgcacc
    10261 ccaatatttt attcctcgcc gacctggcgc aaattatttc aactgctggt gagcacagcc
    10321 taccttaata tggcagtggg tggggctttt ttcgccctgg gcgcactcct gggcatcacc
    10381 tgggtgcgct ggtttggctt gatcatcgcg gtcatccagg tcagcgccat tctgctttgc
    10441 ctgcacgcgg atttcactcc cttctccccc ggcgccaacg acaacgcttc cggtgtcggc
    10501 gtgatccttg agattgcaag gcgcctggta aagcagccat tggatttcac cgaggtctgg
    10561 ttggctttta ctggatgcga ggagactggc gcttcgggca tgtcggcatt cctggattcc
    10621 catgccgagg cgctgggtag cgacacagta tatatcatcg tggatgaagc cgggttaggc
    10681 aaactacaat acctgaccaa tgacggtctg gtgatcaagc gcaataccca cccccaggcg
    10741 ctggagctgg caaatcgggt gggcgtctta cttcctgaac cgagggcgac gcctcgcggt
    10801 ggcatcgctt ataccgacgc cgcggtggct accaaacgaa aattgatcgc tctttcatta
    10861 ggatgtttgc ccgaggctca ggcaggtcag agctctcact ggcaccagct cagcgacacg
    10921 attgatacca ttgatcgtca agccctggaa gattcgctgc tgtttacctg gcatcttttg
    10981 cgacaggttg acaattattg agttccccct tacctccgca tcgcgccggg ttttaacatg
    11041 tatggggatc gggacgttct agtgcgggga atctcatttg agcgactgcc ctatcgacgg
    11101 attagggcaa tcgcattagg gcgatttccc acatctgagg agagaaaata gtgtgttttg
    11161 gtgggggtat gcccccacca aaacacccca aacactcttt ttgcgggatt gcgtatgccc
    11221 ctcagggcat atgccattgc cctggttggg gggttttcca attcaaacgt tccgtgtata
    11281 ctaggcatct gaccccaatc atttatccga cgggagggtt ttcatgaagc gtgcagtaag
    11341 catcagcatt ggatcatcca agcgcgacaa agctgttgaa gtggaattgc tgggggaaaa
    11401 agtgcgcatt gaacgaattg gcacggatgg cgacatggaa aaagccgccc aactctataa
    11461 ggagctggat ggcaaggtgg atgcatttgg cgtagggggg gctgaccttg gagtattagt
    11521 tgatgacaag tggtatccat tgtactcggt acaatctatg gttcgttttg tcgagcagac
    11581 ccccttggtg gatggtgcag gtctcaagaa caccctcgag aatcgcctcg gtccatttat
    11641 cgactcaaag atcggtgatt acgttcaagc tttgggaaag aaggccttca tcaccctggg
    11701 tgttgaccgc tgggggatga gctgttcttt catcgacgcg gggtacgaat gtgtttttgg
    11761 cgatctgatg tttggcctgg ggttgccctt tacaatacgc agcgcccaag cactgaagag
    11821 gctggcgggt attttgatgc cgatagccgg gcggctaccc tttgagtgga tataccccac
    11881 cggtgaaaag caggaagagc gcaaacctaa atgggaatcg tactacaatt ggggaacggt
    11941 gatcgctggc gactgtcact atatcaagcg ccatatgcct gaacgcctag atggcaaggt
    12001 gatcgccacc aataccacta cggtgagtga cgttgaagcc ttccgcaagg ctggagtcaa
    12061 gtatctggtg acatccacgc cagttctcga aggtcgttca tttgggacta atatgatgga
    12121 agcagccttg gtagcggttt cgggtaaagg tcgtgagctg aaacgagacg agctgaatca
    12181 actgctggac cagttaggat ttcaacctga attgcaggag ttgaactgat atggacgcct
    12241 tagtcactgc cggtggtatc cctcaacccg gagaacccct gtacgattat actcagggga
    12301 tatccaaggc gttgttggaa atcgcaggaa agcccatgat ccaatgggtc tttgatgcac
    12361 tggatggggc ggcatccatt caacgtgtgg tgaccattgg tcttgattca gagagcaatg
    12421 tgacatgtca taaattaatc ggatttttac ccaatcaagg tggcatggtg gataatatcc
    12481 gagctggtat ttttaagtta atagaacttg atcccgaacc ccgacctgta ttggtcgtct
    12541 cgtctgatat tccaggaatc acgtctgaga tggtggaatg ggccgtctcc accgccatgc
    12601 aatcgaacga tgacgtatat tacaacgtga ttccccgcca agtgatggag aaaacttttc
    12661 cagaatcgaa gcgcacctac accaggctaa aggatctaga agtatgtggt ggggacatga
    12721 acatcgtgcg gtccagcttg gtgacatcaa aagacgaaat ttgggagaaa ttgatcgctt
    12781 ctcgcaagaa tgtattgaag caagcggcat tgatcgggta tgatacgctc ctgctgttgc
    12841 tcatgcgggg catcactttg caagatgcag ttcaacgggt ggcgcggcgc ctgcacataa
    12901 ccggtagggc ggtcgtctgt ccctatgccg aggtgggcat ggatgtagac aagccccatc
    12961 agcttgagtt aattcgtgcc tttttggaaa ggcggatagt gcagggggct tccgcgagtt
    13021 gagctggcaa aaaagcctaa ttgagttgga tgaacgcata accagttggc tgcgcatagc
    13081 cgaaaggccg ggcttgttgc gcaacttggc aattcttctg gctcactcag gagattcctg
    13141 gttttggctc ctgggtctgg ttttattatg gtggttgggt ggagatttct ggcaagggcg
    13201 agctctggag ctgggggttg gtatcctggt gacggctgtg cttgttatgt tgattaagtt
    13261 ttctgtgcgc agacggcgac ctgtggggga gtggggcaaa atttaccgca acacagaccc
    13321 gcactcgttc ccatcgggtc atgcagcccg agctattctg ctggcggtta tggctgttgg
    13381 attggggcca ggttggtttg cgctgctgct ctggttttgg gcaccgctgg tcgtattggc
    13441 tcgggtagcg atgggtttgc actacttctc agatgtgctg gctggtgcgg ggttggggtt
    13501 tgtgatgggg atagttattt tgcgctttct gcctgcattt cccttttaaa cgtttcgatt
    13561 ggatccacag gcgttgggtc gatcccataa gccatagcca agtaataagc ggtgtagtca
    13621 ccgaaatgca gcccggtcca ttggtttgcc agccggctat caccagtcgc gtcgatgaaa
    13681 tcggtgctaa tgccctctaa catgaaactt ttgcgggtca aatcaaggcg caggcgatgt
    13741 cgggggtgat ccgatgggct acgcaggaat agcgccacca tgcgcggtaa ttgctctggg
    13801 gggtttagga tgcccgccag ggtgttatgg tcggcttccg gcagaagatc gtattgcccc
    13861 caggccttgg cgagctcatt aacctgccct ttccagcggc gggcgaccgg ggcaagaatc
    13921 cccgcgccga ataccgtcac ccagcgcccc atgagctgcc cagcgatacg cttggcagga
    13981 ttcttgacta cgggtgtgct ggcgaccagc tgggcttgct gggctttcat cgcggcagcg
    14041 gcatccatga tttcgtcgga gtgatctggg accaaatcaa ggtgagctaa tgcggaaagc
    14101 agcaacccga aggaatagcc gaccgcggcg cgtggttgtc catgatgttc aaaaacccat
    14161 aatggtgtgc cagcattttt agcagcttcg gccagttttc caccggtgga tatagccagg
    14221 atgcggcaat tgttggcttg ggcttcctta aatgatgaga gcgtctcttc ggtattgccg
    14281 gaatgagatg aggcgatcac cagcgtctcc ggaccgcgtc cccaggcagg caagccataa
    14341 tcgcggtgaa cgatgacagg cactttgcag aaaggttcca cataagcgga tagcaaatca
    14401 cccccaattg ccgagccacc catacccgca atcacaacct ggcggatgcc ttgccagttg
    14461 ggaagtgact ggctcgagcc caacgaccaa gctttctcaa gctggtcagg caagctgtta
    14521 atctggtcgt gcatgtgttg gatatcgact ttggaaaagt aagccaggtc gtctaagttc
    14581 atttgtttta ctcctctctc tcgtgtttat tggcaatcat ccgatagatt tcccgccggc
    14641 tccagcctga ttttgatgcg atgcgcttgg ctagattaga tggggattcg ccttgatcga
    14701 ggccagactg caaggcggag cgcagtttct cttctgtcca gcggggttct tgttgaatgg
    14761 aactccccgc cacgacgatc gtgaattcgc cgcgcggctt ttgagcgcca aagtgtctca
    14821 atgcttcgcc caggctgcct cgatagatct cctcgtgcaa tttggttagc tctctggcta
    14881 tggcgcatgg acgatcgccc agttttgatt gcatgtcttc aagggcaccc atcaagcggt
    14941 gaggtgtttc caggaatatc aatgtaaatg gcatgctggc tacttgtgca agcagattcc
    15001 gccgttccgc ggccttgcgc ggcaggtagc caagatataa aaaggcatct gtaggcaacc
    15061 cagaagccac cagggcggcg atgggcgcag ttggcccagg gatcgggctg acggtgtacc
    15121 cggcggcgat agccgcgcct accaactcat atcctggatc gtttaaggcc ggtgttcccg
    15181 catcggaaac cagcgcaacg tccccctggg ccagcgccgc cagcacctcg tccagtcggg
    15241 tgagcttatt atgctcatga tagctgatca tcggggtgtg gatgccaaag tgagagagca
    15301 gcttgcgtgt gtggcgtgtg tcttcagctg caatcaaacg cgcctcgccc aagatgcgca
    15361 gggcacgagc gctgatatct tcgaggttgc cgatgggagt agcaaccaga tacagtatac
    15421 ccattatggc caggtcagga attccagtct aacggtgaaa ttcactgcgt tgaattcaga
    15481 caggcgagcg cggccgtaac gtccctcatg agtgcggtag caaatatagg aatttaatgg
    15541 gatatcctca accacaatag gagacgtgtc gagggagctg gactggcagt tcgcttgccc
    15601 tggctggctc atgccatata ctcccagcag ggtgctatct tgcggggcaa actggtgctt
    15661 gctgtctatg ggttcgtaaa tgatgtctgc gccgccgccg ttgatttgat cattatccag
    15721 gtcaagagta gctcccacat tcaggttggc tgtgccaacg atgtgaaccg gggaggtggc
    15781 tgtggcggtg gctgtcggcg tggaggtggg ggtaggggtg cgcggcgtgc tgcttggcgt
    15841 ggatgttacg gataccacga tacgcaccca gaacggcgcg gcaccacccg gtccgatacc
    15901 gaagaggaca ttggaggcgt tgcgcagttt ccagttgccc tggtaattac cagctttgga
    15961 tggagcgacc atatccacgg aaatatccac gctctgtcca ggtgctacgt tgcctgcgag
    16021 agctacactg gctggtgcgc ccatttggtc gccggaaaag aatgccaccg cgtaggtatt
    16081 attccaggta caggtacctg tgttttgcaa acgccagact ttggtgaagg attgacctgg
    16141 ctggagaggc gtatcatcgg ggatagtcac atcgattggg ttgccaggag atgcctggtc
    16201 gcaagagaag cgggatgtcg tcgtgggctg gttggcggtc ggctcgcggg tgggagtaat
    16261 ggtgggaggc gtagtgagag gggttgggct ggtgagacca gttgttgggg tgggggttcc
    16321 ttgcgaagac gtgttggatt gtgaattctg ggtcagccta gcctgtaccg tttcatacgc
    16381 ttgtgtgaca tttatgtcgg ttgacgaagc ctcacgcctg ggtatattac aacccgatac
    16441 aatggcgaga ataaggaagg ctagcaggct cgaacgaatt aacgacaatt tcatgggttg
    16501 tcctgatgga gtctacgccc caggttggta ttgtatcccc acaaaccctg ccaggatgtg
    16561 ggcgacctgg cgggcggcct gcgggcgtgc tagccggcga caggcagctt cggcctgggc
    16621 gagctcttgt ggatgagtga tccaattgtg cagggtagcc acaattttct ctggctgggg
    16681 tgcccaaacc cctgccccct cggatacgat atagctcaca ttgccttcct cctgacccgg
    16741 aaggcggctg tagagtatca taggcagccc tgcgttcagc gcctcacaaa tcgtgcctgg
    16801 gccagctttg gtgaccaaaa tatcggccgc acgcatgaaa tttggcattt cgcgcacaaa
    16861 gccgtaaata tgggtaggta tggaccagct catggactcc aggcgggctt ttagactctc
    16921 gtttcgtccg gtgatcacaa ccagcgaggc gttcaagcca gcatcggcga ttgcgcgagc
    16981 ggttttctcc agaggaccca tgccctcacc accgcctact aacaggatga cgggtcgatc
    17041 ctgccgccag ccatattgat ttcgtagctc aaccggatct ccagccgcaa tgcagaagcg
    17101 gttagcaacg ggtagcccca ccacctttac ctggtcaggt ttcaacccat aacgcagggc
    17161 gcgttggcgg gcggcctctg tgggcaccag gcataaatcg gtgagacggt ggtaccaaag
    17221 agcgtgagcg gttaccagat cggttacgac agtgatgtag ggaggcctgt ctttgcctaa
    17281 agagcgcagt ataggtgcat tcgctagagg atgaacggat acgatcaggt cacttgggtg
    17341 ctttgcaatc agcttgcgca gcgctttgcg tacataggga taggtgctgg aggtgagaag
    17401 gcgcgatcgt tgagatccat tggtgagccg gaagccaaag ccccaggctt ggggaatcct
    17461 gaccatcgtc ggatacaaat ccggcatgcg atttagtgga ggcagagcaa tgtctttgaa
    17521 aatatccacc atctcgatcg tgaattgctc ttcgtattcc aaatgcagcg cctcgatgat
    17581 cgcctctgcc gcgctacgat gcccaccgcc agtatcggag aataatatca gtatatgagg
    17641 tttacccggc accggatgtt tcatcacttg ttttcggcaa tatctttttc atgcgcttgg
    17701 caagcgtgcg tcggtcaagt agcacacgcc gaccgcagcc taagcatacc agcccaatat
    17761 ctgcgcccag tcggacaatg cgccactcgt aactgccgca tggatgcggc ttgcgaagtc
    17821 gaacaacatc gtccagttgg atatctggga gcatcaagcg cattataaac tgaaaatcgc
    17881 tctttgcgga tgcttgcatc gagctggatg tgctcgggcg caggggtaga acgcctggtc
    17941 attttggtga tagacgtcgc atgagctgct tttgcaattc aaccacaata aacaaggatg
    18001 tgctaagcag catggcgatg cccaagtcgc gcacgggcaa ggccatcgtc tcgaaaatgg
    18061 attgcaagaa gggtacatag accactgcga tttgtaataa ggtagtcagt atcactgcac
    18121 cgataagcgc ccagttgcta aaaactccga tggtgaagag agaatctcgg ttagatcgga
    18181 tagccagaac atagcccatt tcagataagg tcagggtagt gaagaccatc gtctgccagg
    18241 taggatgtcc catgcgccag taccagaagc ctacacccag ggagaggagc cccatcaacg
    18301 cgccgatgaa caaaatatcc cgacccaccc cgcgcgcaaa gatattctca ttgggcgggt
    18361 aaggggggcg tcgcatagta tttctctcag ccttctctat gcctaacgct aatcctggca
    18421 gcccatcggt gacgaggttg atccaaagga tttgcagcgg gagcagggct agaggcatac
    18481 ccaggaaagg tgccaggatc atgacccaaa tttctccggc attgctggta aatgtatatt
    18541 tgatgaactt gcggatgtta tcgtagatca cccgcccctc ttcgacagct gccacgatgg
    18601 tggcgaagtt gtcgtccagc agcaccatat cggaagcttc tttggataca tccgtgccag
    18661 tgatgcccat ggctacaccg atatcggctt gtcgaagagc tggcgcgtcg tttaccccat
    18721 ctccggtcat agccgcgatc tgcccccgat tttgcagcgc ctgcacgatc ttcagcttgt
    18781 gttccggcga aacgcgggca aacaccgaga ccttcccaac ggtttcctcc agctcttgcg
    18841 cagacatacg atccaaatca tttcctgtgg gcacaccgtt ttcgatatca atctccagct
    18901 ctcgggcgat atagcgagca gtgagcgggt gatccccggt gatcatgatc ggccggatgc
    18961 cagccgttat acatgtctgg acagcctgct ttacctcagc gcgggcggga tcaatcatgc
    19021 ccaccaggcc aatgaaaatt aggccttgtt ccagtttgaa aacatcgtcg ccttctggtt
    19081 gatcgtcaac agcccggctt gccactccga ggacgcgcac tccttccata gccagacgtt
    19141 cgttggcatc ttcgatacgc ttgcgccagc ctgcatcaag tggttcgatg tgattttgca
    19201 cccacacccg gtcggatact tccaggagtc catccatagc acccttggta aatgcgatca
    19261 ggcgcaggtt ctcgggttgc aggttgagca gctccagcag cgtctcctgc catccttcga
    19321 gctgctcact tttgagttca tgcagggtgg tcatgcgttt tcgatcggaa tcgaaaggtg
    19381 cttccgcgac gcgcggcatg atcgcttcaa gggcttcgcg cctcaagccc atgcgcgccg
    19441 cggcaaccat caaggctccc tcggttgggt cgccaacaat gtggaagcgt tgcgaggtat
    19501 tttcatcata ttccagatac gcatcgttgc acaacacact gcctgcaagc atcacggtca
    19561 aggcaggatt ctcattcaaa tatgcgagct gttggtgacc tggtgtttca gaatgggacg
    19621 gctccagatt ctctgaaaaa taacctcgag tttcttcttc actgagatca atgcggtgct
    19681 cggctaggtc aatcacggtc actgtcatgc ggttctcggt cagtgtgccg gttttatcag
    19741 agcagatgac cgtcacggag cccaacgcct caacggcggg caatttgcgg ataagggctt
    19801 ggcggcgtag catgcggcga gcacccagtg ccagggaaat tgtgactaca gccggtaggc
    19861 cctcggggac cgctgcaaca gccaaactta tggcagtgag gaatagcaag cgcaactctt
    19921 cgccacgcgc cacccccagc aaaaatacta atatgaccaa gcccaacgct acccaagcca
    19981 gctgctttcc gagccggttc aggcgctgct ggaggggggt gggttcctgg gcagcagact
    20041 ggatcagttc agcaatatta cctaactcgg tgcgcatgcc tgtctcagcg accacaccaa
    20101 tgccgcgccc gtaggttacc agggtgccca tatagaccat gttataccgt tctgccagcg
    20161 gcagcgattt acccggctgt gcttgcgagc ttttggaaac tggctcggat tcaccggtta
    20221 gggctgcctc ttgagtgcgc atgttaacgc tctccagtat gcggcagtca gctggcacca
    20281 ggttacctgc ctcgagtaaa acgatatcgc ctgggaccaa atcgcgagaa gagatttcga
    20341 gaacgtgccc gcccctgcgc actttgacaa ccggcactgc tagtttcttc agcgcggcca
    20401 tagccttctc tgcttggtat tcctggcgga agcctaatag ggtgtttatc acaacgatcg
    20461 ccaggatggc gactgcatct ttaagatccc ccagaataga tgagataaca gccgagatga
    20521 tgaggatcac caccatggtt tcggtgagtt gttcccacaa aatcagccag ggtgacttaa
    20581 cccctcgctc ctccagctca ttgggaccgt attgctcaag gcggtttgtc gctatttcat
    20641 ctgacaaacc atccatagcg cttgtatcaa gtatgtttag cacttcttcg gtatcttgtt
    20701 ggtaccatgt tcccataatg cctcacaaag gacaaaatca gcttgattgt accttattta
    20761 tatctgcatt ttgcactgtt actatgccct tgcgtgcatg tgtaa
//
LOCUS       DBNGSX010000028         5215 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_70923, whole genome shotgun sequence.
ACCESSION   DBNGSX010000028 DBNGSX010000000
VERSION     DBNGSX010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_70923"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="AC16CB_00940"
     CDS             complement(<1..271)
                     /locus_tag="AC16CB_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3341058.1"
                     /translation="MTTRKIFQITVVLLVAASMVLAACAPQAPKPSGPLVMLDWAGYE
                     IPEFWGEFATKYPDVKVEFSFLTESAEVYSKLQSGFEADLVHPCSN"
     gene            complement(369..1844)
                     /locus_tag="AC16CB_00945"
     CDS             complement(369..1844)
                     /locus_tag="AC16CB_00945"
                     /inference="COORDINATES: protein motif:HMM:NF018012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR3341059.1"
                     /translation="MQPKLTLLSNDLIQRILDEAYQLLMNPGVKVQSPEARQLLAEAH
                     AQVDETNEIAKIPRQVVEKALESVPRQFSLYDRDGQPTVNYGGDAVHFDPGSSGVHVL
                     DPETLEHKPSYSADLVRIIKIAEMLPQYDAQSTAVVCNEIPKAIGDLYRLYLVLLYSK
                     KPIVTGAFSVETLQAMIDMLAIFAGGRAGLEKKPQAVFDVCPSPPLIWSEFGAQNLID
                     LGRAFVPVQIVSMPLAGAAAPVTLLGSVVQHAAECLSGITIHQLAQPGAPIIWGGAPA
                     IFDMRKGTTPMGAVETAMIDAAYAEVGKSLGLPTHTYLGASDAKLVDAQAGLESGITA
                     LIGALAGINMISGAGMLDFLACVSPEKLVVDAEAIAMVKRLRQGMQVHTDTLALAMFE
                     GINFKGDFLKQRITRELFSKEQYIPSKVIDRDSIRGWQQAGSLDTFARAKLRTQALLE
                     EYQPPQIPRAQAEELHSMVAALARRAGMDRLPQISEEAHRR"
     gene            complement(1851..3302)
                     /locus_tag="AC16CB_00950"
     CDS             complement(1851..3302)
                     /locus_tag="AC16CB_00950"
                     /inference="COORDINATES: protein motif:HMM:NF018012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR3341060.1"
                     /translation="MTQRIKSITNPKLKLEILTPEEVRRIHTTTLDIIENVGVRFPSQ
                     KALDIWDAHGAQVNRDSSIVKVPGHIIEEALKKAPSAYPLAARNPSQDLPLDGNHVFL
                     GTDGCGVEVLDIQTGKRRTSCWQDVADIARLGDALPELAFHWVAVSAQDCPPATRGLQ
                     ELRAVWENSTKHVQTESIYHEREAIASIEMAATIAGGREALRVRPVLSLMQCTAPPLG
                     HDGGSLEAALIAAEAGIPTGFMTMAACLTTGPATLAGTLAVGNAEVISATALLQLAYP
                     GAPVFYAAAQTASDPRSGAYTGGGPEDFLFGAATNLLSDFYNIPLSMGSFATGAKEPN
                     WQAGIDNSLSTFMACVVMSDMLLGVGLLHGSRIWSYEQMLMDCEIFSIVHKMMQGIVV
                     DDETLALEAISAVGPGGNFLTQKHSLRHMRELFIPQFMDRRPYNVWEEKGDGSRQWAL
                     EKARRILESHQPEPLDPQLSAELQRIIATVDKG"
     gene            complement(3312..3962)
                     /locus_tag="AC16CB_00955"
     CDS             complement(3312..3962)
                     /locus_tag="AC16CB_00955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HIR3341061.1"
                     /translation="MQTEDLFKAMSQSIIEGESETAAELARQAIELGIDPLEAINAGF
                     VHGINHVGDEFSCGNMFLPELVMAGEAMKTAISVLEPEMSKLGTERQVYGKVVLATVQ
                     GDIHDIGKTLVGTMLSAYGFQVYDMGVDVPITAIVEKAREVDADIVAVSALLTTTMVR
                     QRDVMEALDDMGLRAKVKVMVGGAPVTQEWVQQIGADGYSEDAIGAVSVAKKLMGV"
     gene            complement(4065..4823)
                     /locus_tag="AC16CB_00960"
     CDS             complement(4065..4823)
                     /locus_tag="AC16CB_00960"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3341062.1"
                     /translation="MIDHGEGEFLKRRSLKEEIFQILHQKIVAGKFAPGEWLRQEEIS
                     NQLGVSQTPVREALDLLVSAGLAERIPYRGVRVIQLTTEEIVDAYIMRLILESTAGRL
                     AAHNITPEQIRDINEIIEQTRGLVTLEDMSTQRQLNKKFHMAIVFASGNTLLTRLYEA
                     ASNLFPDWMLYEYMFRHPELLQSSLRREYQEHKAIADAITQHDADQAAQKIVEHIRNL
                     GNEFESFLNIPGEFIREKEQQAAPVWMAKIISSA"
BASE COUNT         1063 a         1428 c         1474 g         1250 t
ORIGIN      
        1 agttggagca cgggtgaacc aggtcggcct cgaagccgct ttgcagcttg gaatagactt
       61 cagcgctttc agtcaggaag ctgaactcca cctttacatc cgggtatttg gtggcgaatt
      121 ccccccaaaa ctcggggatt tcatatcccg cccagtccag catgaccagc gggccgctgg
      181 gtttaggggc ttgcggggcg caggcagcta gtaccatgga ggcggctacc agtagcacta
      241 cggtgatctg gaatatcttt cgggtggtca tttgaactct ccttcactag atgagtcgaa
      301 acgatgaatg gacaattgtt ctgcggtttg cttctcccag tctcctcctt ttggttcagt
      361 tgttcgtttt atcgcctatg ggcttcttcg ctaatttggg gaagcctgtc catccccgcc
      421 ctccgtgcga gcgctgcgac catgctgtgg agctcctctg cttgcgcgcg cgggatttgt
      481 gggggttggt actcctctaa cagggcttgg gtgcggagtt tcgcccgggc aaaggtatcc
      541 aggctgccag cctgctgcca gccacggata gaatcacggt cgatcacttt ggaggggata
      601 tactgttcct tagagaatag ctcgcgggtg atacgctgtt tgaggaaatc gcctttgaag
      661 ttgattccct caaacatcgc cagggccagc gtatcggtgt gcacctgcat accctggcgc
      721 aggcgcttga ccatggcgat agcctccgcg tccaccacca gtttttcggg gctcacgcag
      781 gctagaaagt ccagcatacc tgccccggag atcatattga tccctgccag agcgccgatc
      841 agggcagtga tcccgctttc caggccagcc tgggcgtcga ctagcttggc gtcgctggca
      901 cccaggtagg tgtgtgtggg caggcccaag gacttgccca cctcggcgta cgccgcgtcg
      961 atcatggcgg tttcgacagc tcccatggga gtggtgccct tgcgcatgtc gaagatggcc
     1021 ggcgcgccac cccagatgat cggcgcaccc ggctgggcaa gctggtggat ggtgatacca
     1081 ctcaggcatt cggcggcatg ttgcacaacc gagcctagca aggtgacagg cgcggctgcc
     1141 ccggctagtg gcatggagac gatctggaca ggtacaaaag cgcgccccag gtcaatcagg
     1201 ttctgtgcgc cgaattcgct ccagataagc gggggtgacg ggcacacatc gaagaccgct
     1261 tgcggtttct tttctaaccc agcccgcccg ccggcaaaga tcgccagcat gtcaatcatc
     1321 gcctggagcg tttcaacaga gaaggcaccg gtaacaatag gtttcttgga atacagtagc
     1381 accaggtaca aacggtatag gtctccaata gccttgggga tttcgttgca caccactgca
     1441 gtagactggg cgtcgtactg tgggagcatc tctgcaattt tgatgatccg aaccagatcg
     1501 gcggaatacg aaggtttgtg ttccagcgtc tcggggtcga ggacgtgaac accagaggaa
     1561 ccaggatcga agtgcacggc atctccgccg tagtttactg tgggctgacc atcccggtcg
     1621 tagaggctga actggcgcgg cacactttcc agggccttct cgaccacctg gcgggggatc
     1681 ttggcgatct cgttggtttc gtccacctgg gcatgcgcct cagccagcag ctgccgggct
     1741 tcgggcgatt gcaccttgac cccagggttc atcaagagct ggtaggcctc gtccagtatg
     1801 cgctggatta gatcgttgga gaggagggta agcttgggct gcatgttgat ttacccttta
     1861 tccaccgtag caatgatgcg ttgcagctct gctgatagct gcggatcgag aggctcgggt
     1921 tggtggcttt ccaggatgcg gcgtgctttc tccagagccc actggcgcga tccgtcgcct
     1981 ttttcctccc acacattgta gggtcggcga tccatgaact gggggataaa tagctcgcgc
     2041 atgtgccgga ggctatgctt ttgggtcagg aaattcccgc ctggccccac cgcgctaatg
     2101 gcttccagcg ccagggtctc gtcgtctacg acaatgcctt gcatcatctt gtgcacaatg
     2161 gaaaaaatct cgcagtccat cagcatctgc tcatacgacc agatacggct tccatgcagc
     2221 agccctactc caagcaacat atctgacatg actacgcagg ccatgaaagt ggacaggctg
     2281 ttatcgatgc cggcttgcca gttaggctcc ttggcgccgg tggcgaagga gcccatcgat
     2341 agcgggatat tgtagaaatc ggagagcagg ttggtggcag cgccgaaaag gaaatcttcc
     2401 ggccctccgc cggtgtaggc gccagagcgc ggatcggagg cagtttgcgc ggcggcatag
     2461 aaaaccgggg ctcccggata agccagttgc agcaaggctg tggcgctgat cacctcggca
     2521 ttgccaaccg ccagcgtgcc agctaaagtc gccggacctg tggtcaggca ggctgccatg
     2581 gtcataaaac cggtggggat gccagcttct gcagcgatca gcgcggcttc caggctgccg
     2641 ccatcatgcc ctagcggagg ggcggtacac tgcatgagag agagcaccgg gcgcacccgc
     2701 agcgcttctc tgcccccagc gatagttgca gccatctcga tggaagcgat cgcttctcgc
     2761 tcatggtaaa ttgactctgt ctgcacgtgc ttggtggaat tctcccagac agcacgcaat
     2821 tcttgcaagc cgcgagttgc cggagggcag tcttgggcgg agacagccac ccagtgaaaa
     2881 gccagctccg gcagggcgtc acccaggcgg gcaatatcgg ctacatcttg ccagcaggat
     2941 gtgcggcgct tgccggtttg gatgtctagc acctccacac cgcagccatc tgtacctaag
     3001 aagacatgat tcccgtctaa gggcaggtct tgggagggat ttcgggctgc caaggggtag
     3061 gctgaagggg cttttttcaa tgcctcttcg atgatatgcc cggggacttt gacgatactg
     3121 gaatcgcggt taacctgggc accgtgcgca tcccagatat ctagcgcttt ttgagatggg
     3181 aaacgcacac ccacgttctc gatgatatct aatgtggttg tatggatgcg gcgcacttct
     3241 tctggcgtga ggatttccag tttgagtttt ggattagtga tagattttat ccgctgggtc
     3301 atcgaatgcc tctacacgcc cattagcttc tttgcgacgc tcaccgcccc gatggcgtct
     3361 tcactgtaac catctgcgcc gatttgctgc acccattctt gggtgaccgg cgctccgccg
     3421 accataacct tgaccttcgc tcgcaacccc atgtcgtcca aagcttccat gacatccctc
     3481 tgacgcacca tggtggtggt cagcagggcg ctcacagcaa cgatatccgc gtccacttca
     3541 cgggcttttt caacgatggc agtgatggga acgtcgacac ccatatcata cacctgaaag
     3601 ccgtaagcag acaacatggt gcccaccagt gtcttgccga tatcgtgaat atcgccctgt
     3661 accgtagcca gtactacctt tccatagacc tgccgctcgg tacctagctt ggacatttcc
     3721 ggctctagca cgctgatggc ggtcttcatc gcctcgccag ccatcaccag ctcgggcaag
     3781 aacatattgc cgcagctaaa ctcgtcgccg acatgattga tcccgtgaac gaagcctgcg
     3841 ttgatggctt ccaggggatc tatacccaat tctatggcct gccgggctaa ttcagcggcg
     3901 gtttctgact cgccctcaat aatactctgg gacatggctt tgaataaatc ttcggtttgc
     3961 ataattagct cctgtgcaat gagggttaag ttttgtgaaa ccttgatgat ttaaatttct
     4021 aggcaattca gtgtaaggcc ccatcctccc ttctggatga cttgctatgc ggatgagatt
     4081 atcttcgcca tccaaaccgg ggcggcttgc tgttctttct cccggataaa ttcgcccgga
     4141 atgtttaaaa acgactcaaa ttcgttccct agattacgga tatgctccac gattttttgg
     4201 gcggcctggt ctgcgtcgtg ttgggtgatt gcatcagcaa tggctttgtg ctcctggtac
     4261 tcgcgcctca ggctggattg aaggagctcc gggtggcgga acatgtactc gtagagcatc
     4321 cagtctggga aaagatttga ggccgcttcg tacaatctgg tcaatagcgt attcccactg
     4381 gcgaagacga tagccatgtg gaatttttta ttcaattggc gctgggttga catatcttct
     4441 agtgttacca gcccccgggt ctgctcgatg atttcattaa tatctcgtat ttgttcggga
     4501 gtgatgttat gggcagccaa tctcccggcg gtgctctcca gaatcaggcg cattatgtag
     4561 gcgtctacga tttcttctgt ggtgagctgg atcacacgca ccccgcgata ggggatgcgc
     4621 tccgcgagac cggctgagac aagcaggtca agcgcttcgc gcacaggggt ttggctgaca
     4681 ccaagctggt tggagatttc ctcctggcgc agccactcgc ctggggcaaa ttttccagcg
     4741 acgatttttt gatgaaggat ttggaaaatc tcttccttca ggcttctgcg cttgagaaat
     4801 tcgccttctc catgatcgat catgccgtac ctgccttatt agtatgaagg ggagagccaa
     4861 tggagggtat ggggtggtcg agaaatgttc ctgcattcag ttagtttgta tataatgcat
     4921 tatatataat gtgtagaaca atgtaaagaa acttatccca tgagtttatg taacacaatt
     4981 ggttggtaaa tttcggaaat tggtagagac agaaaggtgt ggagcctctc ccaggctacc
     5041 aagccgcccc agatccggtc agtatcaatg cggtatgtat tcccgaccag gatttgtaac
     5101 ccgcaacgtg cgactgtgcc tctccatgcc gctggcatac tttcacttcg cctactggta
     5161 tctgatcgtc ggcacgctgt tactcctttc gcggcctgat gcagcgcgcc tcgaa
//
LOCUS       DBNGSX010000029        30638 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_417140, whole genome shotgun sequence.
ACCESSION   DBNGSX010000029 DBNGSX010000000
VERSION     DBNGSX010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 30638)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 30638)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30638
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_417140"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="AC16CB_00965"
     CDS             <1..223
                     /locus_tag="AC16CB_00965"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR3341063.1"
                     /translation="GQPVRVGVCVSRLGNKSMDMHYVIEDAESGEELASGSSVVVTYD
                     YHKNLAIPIPDEWRRVISAFDKIQARDKA"
     gene            220..1278
                     /locus_tag="AC16CB_00970"
     CDS             220..1278
                     /locus_tag="AC16CB_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIR3341064.1"
                     /translation="MNQLPPIDSDYLLTFLADLLNTPSPTGYAQRAIDLTQTALEKFP
                     GLTLWRTRKGALVAEWRGARSDSPRALTAHADTLGAMVKEIKSNGRLRLSKIGGFAWN
                     TVEGEGCIVFTSRGETVRGALLLDKASSHVHGKEVNELPRNDQNMEVRLDTRTTNAEE
                     TQALGIQVGDFVAFDPRVEITNGFVRSRHLDDKACVACILAAVKSLYDAGLQPDQTTF
                     LLISNYEEVGHGAAAGIPPQVTELLTVDMAALGKGQTSDEFHATLCVKDSGGPYHHGL
                     SQRLRELADAHGLPYKVDIYPYYGSDGEAFWRAGGDVAVSLIGPGVDASHNYERTHVE
                     ALIATTQWIAAYLLDNRD"
     gene            1303..2304
                     /locus_tag="AC16CB_00975"
     CDS             1303..2304
                     /locus_tag="AC16CB_00975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR3341065.1"
                     /translation="MMNIDASLPPVPLTSIPAIAQAAEQLGFAAVWSTETQHDPFLPG
                     ALIAEHTQRLQFGTAVAIAFARSPATIAYTAWDLAQASQGRFILGLGTQVKAHIERRF
                     GMPWPDSVVGKLREQITAIRAFWHTWQSGERLNHRGEYYKLTLMSPFFNPGPIEHPHI
                     PIYIAGVNTGLARLAGEIADGFHVHPLHTPRYLREVLLPAIEQGATGAGRSRQDLKIA
                     ASPFVVTGDQEDLFVRSQIAFYASTPSYKPVMALHGWQEVAERLSGLAARGEWGEMPA
                     LISDEMLSTFAVVAPTEDVRAALEERYTGLADRLTPYMPFIPGKRDAFWGALLKQAG"
     gene            2311..2982
                     /locus_tag="AC16CB_00980"
     CDS             2311..2982
                     /locus_tag="AC16CB_00980"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR3341066.1"
                     /translation="MDEDVFYTDERQRMVEEQLIYRNIRDERVLEAMRSVPRHAFVPL
                     EYRHMAYSDGPLPIGSGQTISQPYIVALMTQLLRLKGDENVLEVGTGSGYQAAVLAKL
                     AKQVYTIERYTELADQAAAVLQKLGLNNVRVHIGDGSLGLPEHAPFQAILVTAAAPKV
                     PQSLLEQLDEGGRLVVPVGGRMNQFLELWERRGAKYVQDVLVPVAFVPLRGRHGWKED
                     SWEVF"
     gene            3050..3388
                     /locus_tag="AC16CB_00985"
     CDS             3050..3388
                     /locus_tag="AC16CB_00985"
                     /inference="COORDINATES: protein motif:HMM:NF026248.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4491 family protein"
                     /protein_id="HIR3341067.1"
                     /translation="MNLTGFYLGLLMLCAIGFGFLWVIKLEYYLGARVWKAVLGVGVL
                     ICLASLFTPSFTLSAILGILGGSVVWGATELPPQEERVQRGQFPANPKRTAGRTGGEN
                     NIPPHERVES"
     gene            3385..3651
                     /locus_tag="AC16CB_00990"
     CDS             3385..3651
                     /locus_tag="AC16CB_00990"
                     /inference="COORDINATES: protein motif:HMM:NF026248.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4491 family protein"
                     /protein_id="HIR3341068.1"
                     /translation="MSLQWAGVILAVVTFATIAAGHVLVRRLHARYGTRPALPLFVIG
                     GLVLIGSLATKDNLLSGALGITAITILWDGIEIYRQEKRMKKNG"
     gene            complement(3705..5363)
                     /locus_tag="AC16CB_00995"
     CDS             complement(3705..5363)
                     /locus_tag="AC16CB_00995"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HIR3341069.1"
                     /translation="MSKKNNHGGKLRIIPLGGLGEVGKNMMAYQYEENILVVDAGLMF
                     PENDMLGIDYIIPDFEYLIQNRQKIRGVIVTHGHEDHTGAIHHLMQQINAPIYATPLT
                     RGLVEIKLSRGGLLDKIQLNTIQAGEKVQIGPFSVEFFHVCHSIPDGVGLGITTPAGL
                     VIHTGDYKFDHTPVDNWPTDYAKLADFAGRGVLALLADSTNSDKPGWTPSERVIDPGF
                     DQVFREAKGRIIIASFASLISRMQQVANAALRHHRKMAFVGTSMVENARMARKLGYLD
                     IPDDLLVSVDQALKMSPKDIVLMSTGTQGEPSSIMGRLSTGTNRQFDIIPGDTVVLSS
                     HPIPGNEESVYRTINRLFRRGANVIYEAVAPVHVSGHASQEEMKLMLNLVKPKYFIPI
                     HGELRHLKQHATLAKQVGLPEERIAIVENGQIVEFQNGEMNKGERVPGGYVFVDGAGV
                     GEIGPDIMREREILARDGFVLVNLVLDRNNYRLLEEPEIITRGFVYAPEAEELLEAAR
                     QVVVKTVNIASNGRMQSDLVQTLKSFFYSKTKRRPMVFVTMSES"
     gene            complement(5716..9255)
                     /locus_tag="AC16CB_01000"
     CDS             complement(5716..9255)
                     /locus_tag="AC16CB_01000"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3341070.1"
                     /translation="MNDRYRLDSELGSGGMGVVYRAQDMLLERPVAVKVLNQGGLGSE
                     GHSRLLQEAQAAAKLNHPNIVAIYDAGEVDSLAYIVMELVEGASLYEQRPKTIDEIIP
                     IASQLCAALEHAHSHGIIHRDLKPENVMVTSDGIAKLMDFGLARSVASRLTMEGTIIG
                     TVFYLAPEQALGKELDGRADLYALGAMLYEFCTGQLPFSGDDPFAIITQHLHAPVVPP
                     RAKNDAIPVSLDALVVRLLSKDPDERPASAALVLEMLDEVASAPSRPQVEAPIELSLL
                     DRIVRGRIVGREGELAEATVLWERARRGEGQLLLVSGEPGVGKTRLVREVVTRAEVSG
                     SLALAGECYAEGGAPFAPFAQIVRGAVQDGSQGLDVPEFILADLLLLAPDLRLYYPDI
                     PPNPSLDPQSDKVRLFENVVAFCQVLCQRTPLLLLLEDVHWADSGTLSLLHYLARRTR
                     RLPLLLVATYRETDLDQTRPLQALLHELNRERLGNQLKLRRLDKTSTENMLAALFAEG
                     ITPDFLAGIYQETEGNPFFIEEVCKALVESGKLAYADGRWYRPEMSDLEIPQSIRVAV
                     QSRASNLPVDAQEVLTLAAVLGRQFDYELLAAAGRLEENSLIRSLESAVQAQLIEEMS
                     GARGGVFRFTHALIPPILRESLSGPRRRRYHRQVMAALEELRPDDLESLTHHALESGD
                     LQKGYRYSLQAARKAHALYALDEALQHARHALDATEELDQVDQLVEIYELMGDILFLQ
                     GSYLPCAEAYQQALHLTEEPRQRVELNTKIGTTYIWAQDERGLEYLNTVVRDADPRTQ
                     SNELAHATALLGRFHHYRGLYRQAIKYLAEAQEIAEPFQDPLVLTSTYAAMAGAYQHL
                     SEFDNSIAWARRLTELGERHNYPLAIALGYEFQAECFTFMGFWSDTLQYGGKNRAIGI
                     KIGAQHRIGWADYSLAVAYFGMGDLSRAEELAQNSLDLADRIQDNRLQVLSESQLAVI
                     LADRGNHQAARTYAKRAQQRGEKLGDISMVGTGNQALAYCYAMNGDWTAAGQLMDAVA
                     VALKRSDTLMVPLLYFGFYSQVTLESGDLEKAEGIAGGNLTLAREAQSPHYEAEALRV
                     LAQLRSVQGQREPAKELLDQAIQIFERLGSRLELGKAFYYYGKAQIEWEEPQAARQSL
                     DRAAHIFEDCQAVHWIQITGDLSSEIKRKGHEG"
     gene            complement(9540..11174)
                     /gene="groL"
                     /locus_tag="AC16CB_01005"
     CDS             complement(9540..11174)
                     /gene="groL"
                     /locus_tag="AC16CB_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561045.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR3341071.1"
                     /translation="MAAKQLVFSEEARRRLQRGMDLLATAVVTTLGPKGRNVAIDRKF
                     GSPTITHDGVTVAKEIELEDPFENMGAQLLKEAATKTNDIAGDGTTTSTVLAHSIVTD
                     GMKSLAAGFNPMLLKRGVEAATKAVADQIKAQSIPIENKEDIANVASISAQDRAIGDL
                     IAEVMDKVGKDGVITVEESKGLEFETEYVEGMQFDRGYISPYFITDPEHMEASISDAY
                     LLIHDKKISAAQDIVPVLEKLVQVGKRELVIISEDVDGEALATLVLNKLRGMLNVVAV
                     KAPGFGDRRKAMLGDIATLTGATVISEELGRKLESATMQDLGRAEKIVVTKDDTTIVG
                     GKGDTSQIKGRIEQIRVEIDKSTSDYDREKLQERLAKLSGGVAIIRVGAATETELKEK
                     KHRVEDALSATRAAVEEGIVPGGGVALINAMAALEGVKMDNEDAQVGVNIVRKALEVP
                     MRKIAENAGKDGAVILDTVRRTQKEKKNKKIGYDVLTEEYVDMVKSGVIDPAKVTRGA
                     LENATSIAAMILTTEALITDVPEKEKAPAAPPMPEY"
     gene            complement(11214..11510)
                     /gene="groES"
                     /locus_tag="AC16CB_01010"
     CDS             complement(11214..11510)
                     /gene="groES"
                     /locus_tag="AC16CB_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR3341072.1"
                     /translation="MSMSLKPLGNRVVVEPIEMEEMTTGGIVLPETAKEKPQKGKILS
                     IGPGERDEKGTRVPMDVKAGDVVLYAKYAGTEIKVDGKKLLILKESDLLAIVES"
     gene            complement(11666..13105)
                     /locus_tag="AC16CB_01015"
     CDS             complement(11666..13105)
                     /locus_tag="AC16CB_01015"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3341073.1"
                     /translation="MARQLTGKRPNFRPVKSGSNFTRVMVLLGLILAGLWLLQGVQRG
                     QVEPLFLATPTPTRIASSYIFEAQAYFQAGKLDDPDPPKPAPPNPDAIDTYQKALEVD
                     PTNAQAWAEMARIQTYSINMLSNDAERRARLADALESINKAVELNPDDSTIHAVRAFV
                     LDWNASYATTEEGVAEFLKEAEGEAVRAYQLDPLNALALSFYAEVLLDQQKWIQAEQY
                     AAQATALDPNSMDAHRVYATVLESLGQYNSAIKEYLLASSINPNLTFLYVYVGRNYLR
                     LRLYEQALEYFAKAADINTQLGVQNPIPNLEIAKAYSQQGEFFIAARNAEKALSYDPT
                     NANTYGQLGIIYIRSRNYEGALSALRCAVRGCSAEENEIAMNLVDQELLPNSVAVDGL
                     PMTNLAVAYYYVEYGTVLAFLSRPEQNNCPEAIQVLDGVSAQFSDDPVLMEIVADSQG
                     ICARLGSSGAPPAPPDEMMSDIPTPVVSP"
     gene            complement(13171..14430)
                     /locus_tag="AC16CB_01020"
     CDS             complement(13171..14430)
                     /locus_tag="AC16CB_01020"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3341074.1"
                     /translation="MYLRGPKWSMARRLKKRPSPWRILLLLSFIVIALYFNQVIVPAT
                     PPLFIPTSTPTRSPESVVNEAEQLFEEGKLAQAIAAYQQAVIADPSNPILYVTLARIQ
                     IFTGEYQDALTNAQNALLKNPNNPMAHAVQAYAQSFLGDHLAAEASIKRSLELDPNSA
                     LAHAYYAEILMNKGDFEDFEKAAAESRLAKELDPSSLESHRARGLVLLYTGTENLDEA
                     ITELKAAVAVNDKIADLHLLLGFAYRLKGEKDQAVDELLAAYALNPKDPNIPTEITLA
                     YSNEGQFGKAAQYAEDAVKIDPSDAKLRGNLGVMYYKNNEMDKAIQELALAVRGGTTV
                     EGTPVEGLPLDYGKVAEYYWFYGFALAKRNRCAEAVPVFQALVSGVPDYDLAVENAIA
                     GMDLCKASLGTPEVESGESTPEVTATP"
     gene            14533..15312
                     /gene="trmD"
                     /locus_tag="AC16CB_01025"
     CDS             14533..15312
                     /gene="trmD"
                     /locus_tag="AC16CB_01025"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR3341075.1"
                     /translation="MRFDLFTLFPEVFEPYLQSSILQRARQRGLLEVNLYNIRDWTSD
                     RHHVTDDEPYGGGGGMVMKPEPIFAAVEGILGSPPACPVVLLTPQGRPFNQAVAQELA
                     QQPRLALLCGRYEGVDERVRLHLVSDEISIGDYVLTGGELPALVVVDAVTRFLPGVLG
                     DPEGAWDDSHAMGLLEYPHYTRPPEFRGWRVPDTLLSGDHARIARWRREQALLRTKQR
                     RPDLLARAELSAADRKFLAQLEAQDEGRGAADDSQSTDEQG"
     gene            15353..16090
                     /locus_tag="AC16CB_01030"
     CDS             15353..16090
                     /locus_tag="AC16CB_01030"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR3341076.1"
                     /translation="MDPQTPILWADDGLIAVNKPAGLPVLPDGYHPEVPHLRSVLQPV
                     YGRLWVVHRLDKETSGIAVLARTAKAHQALNTQFQERQVTKVYHALVTGTPTWQERVV
                     DAPLLPDGDRRHRTIIEGLRGKPALTQLRLLERFQDYTLLEARPKTGRAHQIRAHLAH
                     LDYPILGDRLYGGNDLYLSMLKPDFRGGINGECSILGRVGLHAWSLEFLHPTNGDKLS
                     LIAPYPKDFSGALKQLRKYQPRDIPLY"
     gene            16214..17248
                     /gene="hrcA"
                     /locus_tag="AC16CB_01035"
     CDS             16214..17248
                     /gene="hrcA"
                     /locus_tag="AC16CB_01035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR3341077.1"
                     /translation="MPKLTERQKLILALVIRSYIETAQPVGSAHLVRRFNLDFSSATV
                     RNEMQTLTELGYLRQPHTSAGRVPTEEGYRYFVRQLMGQSELPVATKHTIAHQFYQAG
                     HDVDRWMRLAASVLAHHSQAASLVTAPHTEQARFKHLELIATHGRQMLMVMVLSGGDV
                     RQQMLSLAEPVSQEQLSAAAAKINALCQGLTPEGLAGVTAQLDVLEKDVIKLVIEDMR
                     RSQDILGGEVYRDGLTHVLSEPEFAEVDVARKALRILEERSLLEDLLARTIMNDAIGG
                     VQVLIGGEGTWEELRDCSMVLARYGVPGLATGTLGVLGPIRMAYGQTISTVRYVAGLL
                     SDLVVDLHAE"
     gene            17320..17889
                     /locus_tag="AC16CB_01040"
     CDS             17320..17889
                     /locus_tag="AC16CB_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR3341078.1"
                     /translation="MTNESISQSENHEMDQVASGEAQETVEALQATTPDDVQVLKDEL
                     IEWQTKANEYLDGWQRARADYANYKKRVERDQAQAHQNATANAIRRFLEVKDDLELAL
                     KNRPNQGDDAGWADGVELIYRKLAAFLEAEGVSIIETDGKFFDPNFHEAITHEVHPEL
                     ESGQIIAVLKQGYRMGDRVLRPAQVRVAS"
     gene            17905..19809
                     /gene="dnaK"
                     /locus_tag="AC16CB_01045"
     CDS             17905..19809
                     /gene="dnaK"
                     /locus_tag="AC16CB_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR3341079.1"
                     /translation="MGKIIGIDLGTTNSVVAVMEGGEPVVIPSAEGERLVPSVVAFNK
                     NRERLIGRVARNQAVVNPENTIFSIKRFMGRKFSDPEVQRATTRVPYKVAQAPNGDVR
                     VILDEREYSPPEVSAMILGKLKADAEAYLGETVTQAVITVPAYFNDAQRNATKDAGKI
                     AGLEVLRIINEPTASSLAYGLDKKKDELIAVYDLGGGTFDISILDVGDGVFQVRATSG
                     DTFLGGDDFDQRIIDFIADEFKREQGIDLRKDRQALQRLKEGAEKAKIELSSMMQTEI
                     NLPYITADASGPKHLVMTLTRAKLEQLTADLIERSLGPVRQAMKDAGLEPDQVNEVVL
                     VGGMTRMPAVQDAVRKLFGKGPHKGVNPDEVVAVGAAIQAGVLGGEVKDILLLDVTPL
                     TLSVETLGSVASPMIERNTTIPTRKSQIFSTAADSQPQVEIHVLQGERPMANDNKSLG
                     KFILDGIPPAPRGIPQIEVTFDIDANGILKVTAQDKATSRSQNITITASSGLAEAEVE
                     RMRKDAEMHADEDRKRKELIEAHNTADNAIYTAEKSLRDLGDKIPAELKSQVEEHVSK
                     VREVINSEDAEKIRQETEALFSVVQQVGTAAYQQEAPSEQAPSEDEQGDEPDSGPDDE
                     EVVDGEFRNV"
     gene            19886..21016
                     /gene="dnaJ"
                     /locus_tag="AC16CB_01050"
     CDS             19886..21016
                     /gene="dnaJ"
                     /locus_tag="AC16CB_01050"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR3341080.1"
                     /translation="MMTERDYYEILGVPKNASNEELKSAFRRLARQFHPDVNKESDAE
                     ERFKEINEAYAVLSDQDKRSVYDRLGHAGVKGAGGVPDFSVDFSDFADIFGDLFGFGG
                     FTRSSRRQRNAPRRGQDLSYRLDLTFEEAVFGLEKEIEFVRDELCNTCSGSGAEPGTS
                     PVRCTTCNGSGEMRQVRQTLLGSMVQVSTCPTCKGVGETITTPCHTCQGRGLERRTRR
                     KVVNIPGGVDDGTQIRLAGEGQPGLNGGPNGNLYITIHVQPHKFFRRRENDIILDLDI
                     NLAQATLGAEVEIPTVDGPARLKIPSGTQPGKVLRMRSKGVPYLRSNGRGDQLVVVNV
                     DIPTRLTSEQRELFEQLAKSLGSEVRPQERSFIDWLKETLGG"
     gene            21101..22027
                     /gene="prmA"
                     /locus_tag="AC16CB_01055"
     CDS             21101..22027
                     /gene="prmA"
                     /locus_tag="AC16CB_01055"
                     /inference="COORDINATES: protein motif:HMM:NF018076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR3341081.1"
                     /translation="MGNSSWLEISLIVDGEMAEAVAEVLGRYVSGGVAIESTAVTARP
                     DDSEGQAIGPLRVCGYLPVDDQLDENRQRVEQALWFLGRIRPLPAPQYHIIHEQDWSQ
                     AWKSHYHPIAIGQRLMVVPAWEQPPDPVRIAIRMDPGMAFGTGTHPTTQLCLELMEIY
                     LPGREAAIIDLGCGSGILAVAALKLGAEQALGVDIDLEAVQATRDNAELNGVAGQLEI
                     GKGSVDEIRRGEFALRRANLVLANILAPVIIRLLDDGLPDLLTPDGVLILSGILEEQA
                     VEVEDALQRYNLRTLDRRQSGDWVALVAGMIL"
     gene            22035..22466
                     /locus_tag="AC16CB_01060"
     CDS             22035..22466
                     /locus_tag="AC16CB_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341082.1"
                     /translation="MNENTFPPSAPKPNPVTQRKHRQEVLWQITLPLALGALLVLAAG
                     AGVVYAGATQTGPVDRWASISIIWLIIPTMFLTLIFLVVTAGLAYGIIWLIGALPKYT
                     RQIQDVFVLIDARVRKAADAIVEPTLRVQSFTAGLRALRRK"
     gene            22482..22712
                     /locus_tag="AC16CB_01065"
     CDS             22482..22712
                     /locus_tag="AC16CB_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341083.1"
                     /translation="MSNNGNEQAGGNWKSKALLAGAVLGAVVGLSAAYLIIQRAERED
                     GEVRISPGDGVKLGVLVFGLLRQVAQLGEGEK"
     gene            22797..23135
                     /locus_tag="AC16CB_01070"
     CDS             22797..23135
                     /locus_tag="AC16CB_01070"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HIR3341084.1"
                     /translation="MEELKEMKCVPCRGGVPPLTSEQIAGLHPGVPEWQVLDVEDIQR
                     LQRVFKFKNFAQALEFTNKVGALAEEEDHHPALLTEWGKVTVTWWTHKIKGLHNNDFI
                     MAAKTDALVQ"
     gene            23373..23999
                     /locus_tag="AC16CB_01075"
     CDS             23373..23999
                     /locus_tag="AC16CB_01075"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3341085.1"
                     /translation="MSEEIAPRGERTRNDILQAAYQLFLERGFHGTSMRQIAQAAGIA
                     LGGIYNHFSSKEDIFTAVFLERHPFRDVLPLMNTAQGETVEEFVRDAAQRMVAGLGQR
                     TDFLNLMFIELVEFKGQHVPHIFQIIFPEMMEFTQRFQTERQELRDIPLLVVVRAFIG
                     LFFSLLITEIMIADQLPPEEREHALDYFVDIYLYGILKGGTTLEKVQQ"
     gene            23996..25126
                     /locus_tag="AC16CB_01080"
     CDS             23996..25126
                     /locus_tag="AC16CB_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3341086.1"
                     /translation="MNSRLTSLIRKEFLQILRDPRTLALALVMPLVQLFLLGYSATND
                     VRNVPLAVYNQDRGPEARALLDAYRAANYFSLEYDVSSEQELRDLIDRGQARGGLVIP
                     PDYSSRLQGDGQAEVAFILDGSDPTVAATALSAAQVIGQQHATSVLSDRLGRRGQSSA
                     IRPPIEVRTQVWYNPDLESAYFMIPGVIGMILYAITSILTSTAIVRERERGTIEQLIV
                     TPIRPWELVVGKMLPYVILAFLNTLEVLAIGALWFHVPIRGDLGLILLLSALFLLTSL
                     GIGLLASTIANTQQEAMLTVWMTLLPSIFLSGFFFPLEAMPKVLQWLSYLMPLRYYLV
                     IIRSLLLKGVGADMLKSDILALAIFGFAIMAAAAMRFRKRLD"
     gene            25145..26491
                     /locus_tag="AC16CB_01085"
     CDS             25145..26491
                     /locus_tag="AC16CB_01085"
                     /inference="COORDINATES: protein motif:HMM:NF052764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR3341087.1"
                     /translation="MHPNPRKIVPIVLLIAILGGAIWYFTLGQGNGNSSALTASGTIE
                     GTQVVIAPELGGRVREVMVQEGEVVETGQTLVRFDDSLLQAQLSQARAALHLTQANYD
                     LAAKGPTDEQRRLAIASAEMELTTARQALQALYDNSGLTESQLEQSIAAIDKSRDKAQ
                     QYLDNVNTEADPSDIDAAWASVVIAQDKLEDALEDFEPYEKKDQSNLTRAIFQAQVAS
                     AQKQYDSMVERYNNLVGTANQYELALARSEVSLAEAKLADAKRQYEELQEGIDPDSLA
                     LAQAHLNTAEANLAVAKADPSPEQLAAAQAQVEVAQGAMEVIEAQIAKLVINAPMDGV
                     VLTRTAEPGEVLVPGAPLLSLLRLDDLTITVYVPEDRYGTIKLGQTASVTVDSYPSEA
                     FEAMVIRIADQAEFTPRNVQTEAGRRTTVFAVELSVTNPEGKLKPGMPADVDFSLK"
     gene            26557..27504
                     /locus_tag="AC16CB_01090"
     CDS             26557..27504
                     /locus_tag="AC16CB_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3341088.1"
                     /translation="MHKSFGETRAVDGLNLRVAAGEIYGLVGPDGAGKTTTLRLLCAA
                     LQLDRIKDDEQSSIRISGYDAVRQTELVRAQIGYLPQRFSLYEDLTVLENIRFFAEVR
                     GMSSADWRPRCMEILGFVGLDPFVDRLAGHLSGGMKQKLGLAAALVHRPRVLLLDEPT
                     TGVDPVTRQDFWQLIIRLASQANHDHAEVAVLVSTPYMDEAARCTRVGFMRAGRLLIE
                     GSPADLRLRLKDQILELRGQPLDMLRHLAHADQDVEDVQMFGDRIHLRTRLGSRDLVE
                     ERLTQRILAEGGEITLLRPVAPQLEDVFISLLDQPNSSS"
     gene            27564..28499
                     /locus_tag="AC16CB_01095"
     CDS             27564..28499
                     /locus_tag="AC16CB_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3341089.1"
                     /translation="MSIEVIHATNLTRRFGDFTAVDHISFTMNAGEVVGYLGPNGSGK
                     TTTIRMLLGLLIPTEGEASVLGYDVVRQAEQIRERVGYMSQKFALYHDLTVGENLAFY
                     AGVYGERSPARLAEVLELVGLSQLEGERVSDLPVGWRQRLALGTAIVHRPKLLFLDEP
                     TSGVDPTTRRAFWDLIYALVDEGVTALVTTHYMDEAEYCGRVGIMRDGKLLAMDAPST
                     LKTSALPGLTWDVFLDGVSSTGASSLLVALNALETCPCVLRAGLVSDHLRAITPADVS
                     AETLRQHLDEAGIEGLRIDPAEPTLEDVFLALATR"
     gene            28596..29615
                     /locus_tag="AC16CB_01100"
     CDS             28596..29615
                     /locus_tag="AC16CB_01100"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR3341090.1"
                     /translation="MESKLPRFIIAGSLLREYVILPSGEALLDVPGGNVLYAAVGLKL
                     WEPEPPPAVLARVGEDYPQEWLDQFTRRGLDTRGVNVISQPVDLRAFTAFLSRTNRAT
                     DDPVAHFARAGLTFPKELLGYRDSSSDLNSRTNLSSISLRQGDIPADYLDASAAHLCP
                     LDYLTHSLLPAVLRQAEFTTVTLDPSPGYMNPTFWDDVPALVTGLTAFLPSEEEMSSL
                     FQGRSTDLWQMAEGLASYGCEIIVIKRGEGGQLLYDAAGRARWEIPAYPSRMVDPSGA
                     GDAFCGGFLAGYRRTYDPLQAALHGNISASLVVEGNGPFYALDCLPALPQARLEALQQ
                     GVRKI"
     gene            29612..>30638
                     /locus_tag="AC16CB_01105"
     CDS             29612..>30638
                     /locus_tag="AC16CB_01105"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HIR3341091.1"
                     /translation="MSSVLGLALSERVLALAMTIQQIPAPTFSEARRAAFIFERFQAE
                     GLSDISMDAIGNVYGRLPGAGSTPPLVVSAHLDTVFPEDTPLDCRREAETIYGPGIGD
                     NALGVAGLFGLLWGVKEIVSPRLQPNLSGPKAKSSLALPGDLWLVANVAEEGMGNLLG
                     MTAVVDRFGGDARAYLVLEGMALGQVYHRGLNVRRYQISASTAGGHSWVDYGQPSAIH
                     ELAGLITRLCDLSLPSQPRTTLNVGVISGGTTVNSIAAEANCLLDLRSEGHESMVNLA
                     TEVEGLIDAGNRSGVQFSWEVVGQRPAGEMPRDHPLVQLAASCLESQGIQPLFNIGST
                     DANLPLSR"
BASE COUNT         6618 a         8584 c         8829 g         6607 t
ORIGIN      
        1 cgggcagcca gtgcgcgtgg gggtgtgtgt ctcaaggctg ggcaataaaa gcatggacat
       61 gcattacgtc atcgaggacg ccgagagcgg cgaggaattg gccagcggct catcggttgt
      121 ggtgacatat gattatcaca aaaacctggc gatccctata ccggatgaat ggaggagggt
      181 gatcagcgct ttcgacaaaa ttcaagcaag ggacaaggca tgaaccaatt accccccatt
      241 gacagcgatt atctccttac gtttctagcc gacctgctga acacacccag ccccacgggc
      301 tacgcccagc gggcgattga cctgacccaa acagcgctag agaagttccc tgggctaacg
      361 ctgtggcgca cacgcaaagg ggcattggtg gctgagtggc gtggcgccag atcggattca
      421 ccccgcgccc tgaccgcgca cgctgacacc ctgggtgcga tggtcaaaga gatcaagagc
      481 aacgggcggc tcaggctgag caagatcggc gggttcgcct ggaatacggt ggaaggcgaa
      541 ggatgtatag tgtttaccag ccggggagaa accgtgcgcg gcgcattgtt gctcgacaag
      601 gcctccagcc atgtgcatgg caaagaggtc aatgagctgc cccgcaatga ccagaatatg
      661 gaagtccgcc tggatacccg taccacgaac gctgaggaga cacaagctct aggcatccag
      721 gtaggcgact tcgtggcctt cgatcctcgc gtcgagataa ccaatggctt tgtgcgctct
      781 cgccacctgg acgacaaagc ctgtgtagcc tgcattttgg cggcggtcaa gtcgctttat
      841 gacgctggct tgcagccaga ccagaccaca ttccttctga tcagcaacta cgaggaagtt
      901 gggcatggcg ctgccgctgg cataccacca caggtgaccg agctgctgac tgtggatatg
      961 gccgctttgg gtaaagggca gacctctgat gagttccacg ccacgctgtg tgtaaaggac
     1021 tcgggcggac cgtaccacca cggcctgagc cagcgcctgc gtgagctggc agacgctcac
     1081 ggcctgccat acaaagtcga tatctatcca tattacggct cagatggcga agctttctgg
     1141 cgcgctggcg gtgatgtggc cgtgtcgttg attggtccgg gcgtggacgc ctcgcacaat
     1201 tacgagcgca cgcatgtgga ggcgctgatt gctaccacac aatggattgc tgcttatctg
     1261 ttagacaaca gagactagat gaataagagt agtacaaatt gaatgatgaa tattgacgca
     1321 tcccttcccc cggttccatt aacttcaata cctgctatcg cccaggctgc agagcagttg
     1381 ggctttgcgg cggtgtggtc tacagagaca cagcacgatc ccttcctgcc gggggcgctg
     1441 atcgccgagc atacccagcg cctgcaattc ggcacagccg tggcaatcgc ctttgcgcgc
     1501 agcccggcaa ccatcgctta tactgcctgg gacctggcac aagcctccca gggtcgcttt
     1561 atcctgggct tgggcacaca ggtaaaagcg cacatagagc ggcgtttcgg gatgccctgg
     1621 cccgattcgg tggtgggcaa actgcgcgag cagatcaccg ccatacgcgc cttctggcat
     1681 acctggcaaa gcggcgaacg gctcaaccac cgcggagaat attacaagct cacgctgatg
     1741 tcaccattct tcaaccccgg cccaattgag cacccgcata ttcccatcta catcgctggg
     1801 gtgaataccg gtctggctag attggcaggt gagatagccg acggcttcca cgtacacccg
     1861 ttgcatacac cccgctacct gagggaggtg ctgctgcccg ccatagagca gggggcaacc
     1921 ggggcaggtc gatccagaca agatctaaag atcgccgcct cgccctttgt ggtcactgga
     1981 gaccaggaag accttttcgt gcgctcacag atcgccttct acgcttccac gccgtcttac
     2041 aagccagtga tggcgctgca cggctggcag gaggtcgccg agcgcctctc tgggctcgcg
     2101 gcgcgcggcg agtggggtga gatgccagcc ctgatcagcg acgagatgct atctaccttc
     2161 gctgtggtcg ccccaacaga ggacgtgcgg gcggcattgg aggagcgata caccggtctg
     2221 gcagaccggc tgacacctta tatgcctttt atcccaggta aacgagatgc attttgggga
     2281 gcccttttga aacaagctgg ctgagcagcc atggatgagg acgtttttta caccgacgag
     2341 cgccagcgca tggtagagga gcagctcatc taccgcaaca ttcgcgatga gcgtgtcctg
     2401 gaagctatgc gatctgtacc ccgccacgcc tttgtcccct tggagtatcg ccacatggcc
     2461 tacagcgacg gtccgttgcc catcggcagc gggcagacga tctcccaacc ctatattgtc
     2521 gccttgatga cccaattgct gcgcttgaaa ggagatgaga acgtgctgga agtgggcaca
     2581 ggctcaggat accaggcagc agtgctggcc aagctggcca agcaggtgta caccattgag
     2641 cgctacactg agctagcaga tcaagccgcg gccgtgttgc agaaactggg gcttaataac
     2701 gtacgcgtgc atatcggtga tggctccttg ggattgcctg agcacgcccc cttccaggcc
     2761 attctcgtca ctgcagccgc gcctaaagtc ccccagtcct tacttgagca gctcgatgag
     2821 ggtgggcggt tggttgtgcc ggttggcggt cggatgaacc agttcctgga gctatgggag
     2881 cgacgcggag cgaagtacgt ccaggatgtg ctggtaccag tggctttcgt gcccctgcgc
     2941 gggcgacacg gatggaagga agattcctgg gaagtttttt aacaaggggc gacaaagaat
     3001 ctgagttctt tgggatttgc atagtctggg caagatatga ggttgtgacg tgaatttgac
     3061 tggcttttac ttgggtttgt tgatgctctg tgccattggc ttcggtttct tgtgggtgat
     3121 aaagctcgag tactatcttg gggcgcgagt gtggaaggct gtgctgggcg tcggtgtgct
     3181 gatctgcctg gcttcgctgt tcacgccgtc gttcacacta tccgccatcc tgggcatttt
     3241 gggtggatcc gtggtgtggg gggcgaccga actaccccct caagaagagc gtgtacagcg
     3301 cgggcagttc ccggctaatc ccaagagaac agcggggaga actggcggag agaacaatat
     3361 tcctcctcat gaaagggttg agtcgtgagc ctccaatggg ctggtgtgat actggcggtg
     3421 gtcaccttcg ccaccatcgc cgccggacac gtcctggtgc gccggctgca cgcccgctac
     3481 ggcactcgcc cggcgctgcc tctctttgtt attgggggat tggtgctgat cggctcgctt
     3541 gcaaccaagg acaatctgct ctccggcgct ttaggcatca ccgccattac cattttatgg
     3601 gatgggatag agatctaccg ccaggagaaa cgaatgaaga aaaacgggta aatgggcgac
     3661 gataccgcct acatttaccc gtttttcgat caatcagctt gttattatga ttcgctcatg
     3721 gtcacaaaaa ccatggggcg gcgcttggtt ttgctgtaaa aaaacgactt cagcgtttgc
     3781 accaggtcag actgcatgcg tccattgctg gcgatattga ctgtcttgac aaccacttgt
     3841 cgggcagctt ccagcagctc ctcggcttct ggggcataga caaacccacg cgtgataatc
     3901 tctggctctt caagcaagcg gtagttattc cgatcaagca ccaagttgac cagaacaaag
     3961 ccatcacgag ccaggatctc gcgctcgcgc atgatatctg gtccaatctc gcccacgccg
     4021 gctccatcca caaaaacgta accgccaggc acgcgctcgc ctttattcat ctcgccgttt
     4081 tgaaattcaa cgatctgacc attttctacg atggcgatgc gctcttcggg gaggcctacc
     4141 tgcttggcca gggtggcgtg ctgcttgaga tggcgcagct ccccatggat ggggatgaaa
     4201 tatttaggtt tgaccaggtt gagcatcagc ttcatctctt cctggctggc gtgtcccgag
     4261 acatgcactg gggcaaccgc ttcatagatc acattggcgc cgcggcggaa gagacggtta
     4321 atcgtgcgat agacgctctc ctcgttgccg ggtatagggt gtgacgagag caccacggta
     4381 tcgccaggga tgatgtcaaa ttggcgattg gtgccggtgg ataggcgccc catgatcgaa
     4441 gacggctcac cctgggtacc agtggacatc aagacaatat ccttcggact catcttgaga
     4501 gcttgatcca cgctcaccag caaatcgtcg gggatatcca aatagcccaa tttacgcgcc
     4561 atgcgtgcgt tctcgaccat gctggtgcct acaaaggcca ttttccgatg gtggcgtaaa
     4621 gcggcgttgg caacctgctg catgcgcgaa atcaacgagg cgaagctggc gatgatgatg
     4681 cgccccttgg cttcccggaa aacctggtca aagcccgggt cgatcacccg ctcggagggg
     4741 gtccaaccgg gtttgtcgga attggtcgaa tcggcaagca aagccagcac gccgcgtccg
     4801 gcaaaatctg ccagcttggc gtaatcggtg ggccaattgt ccaccggggt gtggtcgaac
     4861 ttgtagtctc cggtatggat caccagacca gcaggggtgg tgatgcccaa gcccactcca
     4921 tcggggatgg agtggcagac gtggaaaaac tccacgctga aggggccaat ctggaccttc
     4981 tcgccagcct ggatggtgtt caattgtatt ttatccagca agccgcctcg cgagagcttg
     5041 atttccacca agccgcgcgt cagtggtgta gcatagatgg gtgcgttgat ctgctgcatc
     5101 aggtgatgga tggcaccagt gtgatcctcg tgcccatggg tgacgatgac cccccgaatt
     5161 ttttgacggt tttggatcaa atattcaaaa tcggggatga tatagtcgat ccccaacata
     5221 tcgttttcgg ggaacatcag gccagcgtcc acaacgagaa tattctcctc gtactggtag
     5281 gccatcatgt tcttaccgac ctcccccagg cctcccaggg ggataatgcg gagtttacct
     5341 ccatgattat tctttttact catatctttc tataatcctt actaagtatt aacctctccc
     5401 aggttaagaa cctgcggaag gttcatttgt cgtaatataa acgccccggc gggttgactc
     5461 gatagtctgt gaacatccgg ccggggcgca atatacaaat tggcattgat tcaatgcgta
     5521 atatgtttcc cctcacattc cttctggttg tttgaaccat ggtacatgat ggggccggag
     5581 taccggccag gcgtgactca cgaacgaaaa gaattgtagc atgatggata ggttttgtca
     5641 aagattaatt gggcaatatc tatgaattat taacacaatg agcacgaagt atcaataagg
     5701 gcccaactta tttcttcatc cttcgtgtcc tttgcgttta atttcgctac tcaaatcccc
     5761 agttatttga atccaatgaa ctgcctggca atcttcaaag atatgtgcgg ctcgatcgag
     5821 ggattggcgg gctgcctgtg gctcttccca ttcaatttgc gcctttccat agtagtagaa
     5881 cgctttgcca agctccaggc ggctgcctaa gcgctcgaaa atctggatcg cctggtcgag
     5941 caattccttg gctggctcgc gctgaccctg cacgctgcgc agctgcgcca agacacgcaa
     6001 agcctctgcc tcataatggg gcgattgagc ctcccgcgcc agggtcagat tgccgccagc
     6061 tatgccttca gctttctcca ggtcacctga ctcaagggta acctggctgt agaaaccaaa
     6121 atacagcaaa ggtaccataa gggtatcgga tcttttcaga gcaaccgcaa ccgcatccat
     6181 taactgacct gccgctgtcc aatcaccgtt catcgcatag caataagcaa gagcttgatt
     6241 gcctgtaccg accatagata tgtcccccag cttttccccg cgctgctggg ctcgtttggc
     6301 atatgtcctc gcagcttgat gatttcccct gtctgctaag atgaccgcaa gttggctctc
     6361 ggaaagcact tgcaaccggt tatcttgaat gcgatcagcc aggtccaagg agttttgagc
     6421 tagctcttcg gcgcgcgaca gatcacccat gccaaaatat gcaaccgcca gggaatagtc
     6481 ggcccagccg atgcgatgct gggcgccgat cttgatgcca atggcacggt ttttaccgcc
     6541 atactgaagg gtgtctgacc aaaatcccat aaatgtgaaa cattcagcct gaaattcgta
     6601 acccagggcg attgccaatg gataattatg gcgctcgcct aattcagtca aacgacgcgc
     6661 ccatgctatg ctgttgtcaa actcactcaa gtgctggtac gcccccgcca tcgctgcata
     6721 ggtggacgta agcacaagcg gatcttggaa tggctcggcg atctcttggg cctccgccag
     6781 atactttata gcttgccgat acaggcctcg gtagtgatga aacctgccaa gtaatgcagt
     6841 tgcatgcgct aactcattcg actgggtgcg tggatcggca tcgcggacga cagtattcag
     6901 gtactcaagc ccgcgctcat cctgggccca gatataagtt gtgccaatct ttgtattcaa
     6961 ctcaacgcgc tgccgaggct cttcggtgag atgcagtgcc tgttggtacg cctcagcaca
     7021 gggcagatac gacccttgta gaaagaggat atcacccatc agctcataga tttcgaccag
     7081 ttgatccacc tgatcgagct cctccgtcgc gtccaaggcg tggcgggcat gctgcaaggc
     7141 ttcatccagc gcgtaaaggg cgtgggcttt tctagcggcc tgaagcgaat aacgataccc
     7201 tttctgcagg tcgccgctct ccaaggcatg atgggttagg ctttccaggt cgtcgggacg
     7261 cagttcttcg agggctgcca tcacctggcg gtgatagcga cggcggcgtg gaccactcag
     7321 cgactctctc aagatgggtg gaatgagcgc atgtgtgaaa cgaaatactc caccgcgtgc
     7381 gccgctcatc tcctcgatga gttgggcttg aacagcgctc tccagggacc ggatcaagct
     7441 gttttcctca aggcgcccag ccgctgccag taactcgtaa tcaaactggc gccccagaac
     7501 tgcggccagg gttagcactt cctgtgcatc gactggcagg ttgctcgccc tggattggac
     7561 agccacgcgt atgctctgcg ggatttccag atcgctcatt tccggacgat accagcgccc
     7621 atcagcataa gcaagcttac cactctccac cagcgccttg cagacctctt cgatgaagaa
     7681 ggggtttcct tccgtctcct gataaatccc tgccaggaaa tcaggcgtaa tcccttcggc
     7741 aaagagagcc gccagcatat tttcagtgct ggttttgtcc aggcggcgca atttcaactg
     7801 gttgcccaat cgctcccggt tcagttcgtg caacaaggcc tgcagcgggc gggtctggtc
     7861 gagatcagtc tctcggtagg tggcaaccaa caggaggggc aggcggcgcg tccggcgagc
     7921 caggtagtgg agcaggctca atgtgccact gtcggcccag tgcacatctt ccaacaacag
     7981 caatagcggg gtgcgctggc acagcacctg gcagaacgcc acaacatttt caaaaaggcg
     8041 cactttatcc gattgtggat ccaatgatgg attgggcgga atgtcagggt agtaaaggcg
     8101 caaatcagga gccaagagga gcaggtcggc aaggatgaat tctggcacat cgagcccctg
     8161 cgagccatcc tgcaccgcgc cgcgaacaat ctgggcaaag ggggcaaaag gcgcgccccc
     8221 ttccgcgtaa cattccccag ccagtgccaa gctcccagag acctcagcgc gggtgacgac
     8281 ttcgcgcacc aggcgggtct tgcccacccc cggctctcca ctaaccagca ggagctggcc
     8341 ctcgccacgg cgagcgcgtt cccacaggac agttgcttca gccagctcgc cttctcgccc
     8401 cacgatacgc ccgcgcacaa tgcggtcgag cagggatagc tctatcggcg cctcgacctg
     8461 gggtctagac ggcgccgaag cgacttcatc tagcatttcc aaaaccagcg cagcagatgc
     8521 cgggcgctcg tctggatcct tgctcagcaa ccggacgacc aaagcgtcca aagagacggg
     8581 aatagcatcg ttctttgccc gcggtgggac taccggggcg tgcaggtgct gagtgataat
     8641 ggcaaagggg tcatcaccgg agaagggaag ttgaccggtg caaaattcat acagcatggc
     8701 acccaaagca tacaggtcgg cacggccatc cagttctttt ccaagcgcct gctcaggggc
     8761 caggtagaag accgtgccga tgatggtgcc ttccattgtg aggcgcgagg caaccgagcg
     8821 ggccaagccg aaatccatca gcttggcgat gccatccgag gtgaccatca cgttttcagg
     8881 cttcagatcg cggtggatga tgccatggct atgggcatgc tcaagcgcag cgcacagctg
     8941 gctggcgatc gggatgattt catcgatcgt cttgggtctt tgctcataca acgatgcccc
     9001 ctcaaccaat tccatgacga tgtaggctag gctatcgact tctccagcat cgtaaatcgc
     9061 tacgatattg ggatggttaa gctttgccgc cgcctgggct tcttgcagca accgcgagtg
     9121 tccttcgctg cccaaccctc cctggttcag aactttgacg gcgacgggcc gttcgagcag
     9181 catatcttgg gcgcgataga ccacccccat cccgccacta ccgagctcgc tatccaaacg
     9241 gtagcgatcg ttcaataaca tccccatagc tatcatggcg attactctac ccttctctgg
     9301 atagacaggt tttccgcagc cgaacccaac atgcgacgcc tgcccatcta tataaatttc
     9361 aggacaaacc cgctaaactt tcgcttatat gtctaatata attttattat acatgccttc
     9421 gcgttttatt tggcataaaa tgaacgggag agggtgagca atcaccatat ctacccgcgt
     9481 gagacaaaaa aatcccccca tcctgtatgg acggggggag caggtgcttt gagtgaattt
     9541 taatattccg gcataggggg agcagccggg gctttctctt tctcgggcac gtcggtgatc
     9601 aaggcctcgg tggtcaggat catggcagcg atggaggtag cgttttctag ggcgccgcgg
     9661 gtcactttgg cgggatcgat cacgccgctc ttcaccatgt ccacgtactc ctcagtgagc
     9721 acgtcgtagc cgatcttctt gttcttcttt tccttctgcg ttcgtcgcac ggtatccagg
     9781 ataacggcgc catctttgcc agcgttctcg gcgatcttgc gcatgggcac ttccaaggct
     9841 ttacggacga tattcacacc cacctgggca tcctcattgt ccatcttgac gccttccagg
     9901 gcagccatgg cgttgatcag ggctacgcca ccgcccggca cgatgccctc ttccacggcg
     9961 gcgcgggtgg ccgaaagggc gtcttcgacg cggtgcttct tctctttcag ctcagtttca
    10021 gtggccgcgc ccacgcggat gatggcgacg ccgccggaga gcttggccag gcgctcctgg
    10081 agcttctcgc ggtcatagtc gctggtgctc ttatcgatct caacgcggat ctgctcaatg
    10141 cggcctttga tctggctagt gtcgcccttg ccaccgacga tagtggtgtc gtccttggta
    10201 accacgatct tctcggcgcg gcccaggtct tgcattgtgg cgctctccag cttgcgaccg
    10261 agctcctcgg agatcacggt agcgccggtc agggtggcga tatcgcccag catagccttg
    10321 cggcggtcgc caaagccggg ggccttgaca gcgaccacgt tgagcatgcc acgcagcttg
    10381 ttcaacacca aggttgccag agcttcaccg tccacgtctt cggagatgat aaccagctcg
    10441 cgcttgccta cctgcaccag tttctccagc acggggacga tgtcttgggc ggcggagatc
    10501 ttcttgtcgt gaatcagcag gtaagcgtcc gagatggagg cttccatgtg ctcggggtca
    10561 gtgatgaagt aaggcgagat atagccgcgg tcgaactgca taccttcgac atactcggtc
    10621 tcaaactcca aacccttgga ttcttcgacg gtgatgacgc cgtctttgcc gaccttatcc
    10681 atcacctcag cgatcagatc gccgatagcg cggtcctgag cggagatgga agccacgttg
    10741 gcaatatctt ctttgttttc gattggaatg gactgggcct tgatttggtc agccacagcc
    10801 ttggtagcgg cttctacgcc gcgcttgagc agcatagggt tgaaaccagc tgccaggctc
    10861 ttcataccat cggtgacgat ggagtgcgcc aggacggtgg aggtagtggt accgtcgccg
    10921 gcaatatcgt tggttttggt agcggcttct ttgagcagct gggcgcccat gttctcaaag
    10981 gggtcttcca gctcaatttc tttggcgaca gtgacgccgt cgtgggtaat ggtgggggaa
    11041 ccgaacttgc ggtcaattgc gacgttgcgg cctttcgggc cgagggtggt gaccaccgcg
    11101 gtcgccagca agtccatgcc gcgctgcagg cggcggcggg cttcttctga gaataccagt
    11161 tgtttagcgg ccatagaaca tttactcctt atctaggatg aattcagggg gggttacgat
    11221 tcaacgattg ctaacagatc gctctctttg aggataagca gcttcttgcc gtccaccttg
    11281 atctcggtgc cggcatattt ggcgtacagg acaacgtcgc cagccttgac atccatgggg
    11341 acgcgtgtgc ctttctcatc gcgctcgcca ggtccgatag acaggatttt gcctttttgc
    11401 ggcttttctt ttgcggtttc ggggagcacg ataccgccag tggtcatctc ttccatctcg
    11461 atgggctcga ccaccacgcg gtttcctaaa ggcttgagtg acatagacat gactacctcc
    11521 ttggggtata aggtttgaat ttcaagtgac atgcagaaga gttagcactc agtgtgcgag
    11581 agtgctaact cgcggtcaaa tgatacccat tttttcgatg ggagtcaaga gggagcttga
    11641 aattttaata aaattctgac attccctacg gagatacgac cggtgtaggt atgtcgctca
    11701 tcatctcatc cggaggtgca ggcggcgcgc cactcgatcc caggcgtgcg cagataccct
    11761 ggctgtctgc cacaatctcc atcagtaccg ggtcatccga gaactgagcg ctcacaccgt
    11821 ccaacacctg tattgcctca ggacagttgt tctgctctgg tcggcttagg aatgccaaga
    11881 cagtaccata ttcaacatag tagtaagcca cggccaggtt ggtcatcggc aggccatcca
    11941 cagcgacgct attcggaagc agctcctgat ccaccaggtt catagcgatc tcgttctcct
    12001 cggcgctgca accacgcaca gcacagcgca acgcgctcaa ggcgccttca tagttgcgcg
    12061 acctgatgta gatgatgcct aattgcccgt aggtattagc attggtggga tcgtagctaa
    12121 gtgctttttc agcattgcgg gcggcgataa agaattcacc ttgctgggag taagccttgg
    12181 cgatctccag gttggggatg gggttttgca cacccagctg ggtgttaata tccgcggctt
    12241 tggcaaagta ttccaaagcc tgctcgtaga gtcgcaggcg caggtagttg cgaccgacgt
    12301 agacgtacag gaaggtcagg ttgggattaa tggatgaagc cagcagatat tctttgatgg
    12361 cactgttgta ctgcccaagc gactcgagta cagtggcata aacacggtga gcgtccatcg
    12421 agttggggtc cagggcggtg gcttgtgcag cgtactgctc ggcttgtatc cacttctgct
    12481 ggtcaagcag cacctccgca taaaatgaca gcgcaagggc attgagtgga tccagctggt
    12541 aggcgcgcac ggcttcccct tcagcctcct tcaagaactc ggctaccccc tcctccgtag
    12601 tagcgtagga cgcattccaa tccaggacaa aggcgcgcac agcatggatg gtgctatcat
    12661 ccgggttcag ctcgacagcc ttattgatcg attcaagggc atcagccagg cgggcgcgcc
    12721 gttcggcgtc gttgctgagc atattgatgg aataggtctg gatgcgggcc atctcggccc
    12781 aggcctgggc gttggttgga tcgacctcca gggctttctg ataggtatcg atagcatccg
    12841 ggttgggtgg agcgggcttt ggagggtccg ggtcatccag ctttcccgcc tggaagtatg
    12901 cctgagcctc gaatatatag gacgaagcga tgcgggtggg ggttggcgtg gctaagaaga
    12961 gaggctccac ctgtccacgc tgcacaccct gtaataacca caggccagcc aggataaggc
    13021 ctaataaaac catcacacgg gtaaagttag atcccgattt gactggacgg aaatttgggc
    13081 gcttaccggt gagttgtctt gccatcgtat tgaatcattc aaattcgcgc agttggggat
    13141 tcatttccat caactgcgta aatcctaaca ttaaggggtt gccgtgacct cgggtgtgct
    13201 ctcgccagat tctacttcag gagttcccag gctggctttg cataaatcca taccagcgat
    13261 ggcattctca accgccaggt cataatcggg cacgccagag accagcgcct ggaagacagg
    13321 aactgcttca gcgcaacggt ttctcttcgc cagggcaaaa ccgtagaacc agtaatattc
    13381 agctaccttg ccgtagtcca ggggcaggcc ttccacaggc gtcccctcaa cagtagtccc
    13441 gccccgcact gccaaagcta attcttgaat ggctttgtcc atctcgttat ttttgtaata
    13501 catgacgcct aagttgccgc gcagtttggc atcagagggg tcaatcttga ccgcatcctc
    13561 ggcatattgg gcagccttgc cgaactggcc ctcattggag tatgcgagcg tgatctcggt
    13621 agggatattg gggtctttcg ggttgagggc ataagccgcc agtagctcat ccaccgcctg
    13681 gtctttttca cccttgagac gataggcaaa tcccagcagc aggtgtaggt cggcgatctt
    13741 atcattaacg gcaacagcag cctttaattc ggtgatggcc tcatcgaggt tctcggtccc
    13801 ggtgtagagc aacaccaaac cacgggcgcg atgcgactcc agggaactgg ggtccagctc
    13861 ctttgccagc cgggattcgg ctgccgcttt ctcgaaatcc tcgaagtcgc ctttgttcat
    13921 caggatctcg gcgtaatagg cgtgagccag cgcgctgttt gggtcaagct ccagggatct
    13981 tttgatggat gcctccgcag ccaggtgatc gcccagaaag ctctgcgcat aggcctgaac
    14041 agcgtgcgcc atggggttat tggggttttt aagcagggcg ttttgggcat tggtcagggc
    14101 gtcttggtat tctccagtaa agatctggat acgggccagg gtgacataca agatggggtt
    14161 gctcggatcg gcgatgacag cttgctggta agccgcaatc gcctgtgcca gcttgccctc
    14221 ctcaaagagc tgctcggctt cgttgacaac cgactcggga ctgcgtgtgg gcgtggaggt
    14281 agggatgaac agaggtggcg tggcagggac aatcacctgg ttgaagtaca gggcaataac
    14341 gataaagctt aataggagca agatgcgcca gggactaggg cgcttcttca ggcggcgggc
    14401 catgctccat tttggaccac gcaaatacat attttgatca taccattggc aaggaataga
    14461 cgcaagaaag gtttttattg gctctttgaa aattccttgc caaccgatca tgaaatagca
    14521 tagaattaca ctatgcgctt tgacctattt accctatttc cagaagtttt cgagccttat
    14581 ttacagtcta gcatcctgca acgcgccagg cagcgcggcc tgctggaggt taatctgtac
    14641 aacatccgcg actggacaag cgaccgccat catgtcactg atgacgagcc gtatggcgga
    14701 gggggcggca tggtgatgaa gccagagccg atcttcgccg cagtggaggg catactgggc
    14761 tcgcccccgg cctgcccggt cgtgttgctc acaccgcagg ggcgcccgtt taatcaggcg
    14821 gtagcacaag agctggcaca gcagccgcgc ctggccctgc tgtgcgggcg ttacgagggg
    14881 gtggacgagc gtgtacgcct gcacctggtg agtgatgaga tctccatcgg cgattacgtg
    14941 ctcaccgggg gggaactgcc cgccctggtg gtggtggacg cggtgacccg ctttttgccg
    15001 ggggtgctgg gcgatccaga aggcgcctgg gacgattcgc acgctatggg tttgctggaa
    15061 tacccccact acacgcgccc gccggagttc cgcggctggc gcgtaccaga cacgctgctc
    15121 tcgggcgacc acgcccgcat cgctcgctgg cgaagggagc aagccttact gcgcacaaag
    15181 cagcgacgtc ctgacctgct ggcgcgcgca gaactctccg cagcggatcg caagttctta
    15241 gcccaactcg aagcacagga cgaggggcga ggggcagctg acgattcgca aagtacagac
    15301 gaacaaggct gaccacgcgc tttcaccgga ttaacaggca agcatcttga acttggaccc
    15361 gcaaactcct atcctctggg ctgatgatgg cctcatcgct gtgaacaagc cagccgggct
    15421 gcccgtactc ccagatggct accacccaga agtgccccat ttgcgcagcg tgttgcagcc
    15481 agtttatggg cggctgtggg tagtacaccg cctggataaa gagaccagcg gcatcgccgt
    15541 tctggcgcgc acggctaagg ctcaccaggc gttgaacacg caatttcagg agcgccaggt
    15601 gaccaaggtc taccatgccc tggtaactgg aacacccacc tggcaagaac gcgtggtaga
    15661 tgcgccattg ctgccggacg gagatcgcag gcaccgtaca atcatcgaag gtttgcgcgg
    15721 caagccagca ctgacgcagt tgcgcctgct ggagagattt caggattaca ctctgctgga
    15781 agccagaccc aagacgggcc gcgcacacca aatccgcgcc caccttgcgc acctggatta
    15841 tccaatcttg ggcgataggc tgtacggtgg taacgacctg tatctgtcca tgctcaagcc
    15901 agactttcgt ggcggcataa atggcgaatg tagcatcctg gggcgggtgg gcttgcacgc
    15961 ctggtcgctg gagtttttac acccgacgaa tggggacaaa ctgagcctca tcgctcccta
    16021 tccgaaggat ttttctggcg cgctgaagca gctgcgcaaa tatcaaccac gggatatacc
    16081 cctttattaa ccaaatatca gaattgtcag ctcgcatcac ctcgtttctt gacgttctca
    16141 tgcgggcatg gtattattta ttgcgctttt ttagcactct cacatcgaga gtgctaaatt
    16201 caatgaggtg aaaatgccga aattaaccga acgccaaaaa ctgatcctgg ccttggtgat
    16261 ccgcagctat atcgagactg cgcagccggt cggatcggcc cacctggtgc gacgtttcaa
    16321 tctggatttc agctcagcta cggtgcgcaa cgagatgcag accctgacag agctgggtta
    16381 cctgcgacag ccacatactt cggctgggcg cgtgcccacc gaggagggat atcgctactt
    16441 cgtgcgccag ctgatgggcc agtctgagct gcccgtagcg accaagcaca ctatcgctca
    16501 ccagttttac caggccgggc acgacgtgga tcgctggatg cgcctggctg cttcggtcct
    16561 ggcgcaccat tcgcaagctg cttcgctggt gaccgcccca cacacggaac aggcacgctt
    16621 caagcatctg gagctgatcg ctacgcacgg gcgacagatg ctgatggtga tggttctctc
    16681 tggcggcgat gtgcgccagc agatgctgag cctggcagag ccagtgtccc aggagcagtt
    16741 gagcgctgca gccgctaaaa ttaacgcact gtgccagggg ctcacgcccg agggtttagc
    16801 tggcgtgact gcccagctgg atgtgctgga aaaggacgtg atcaaactgg tcatcgagga
    16861 catgcgccgc tcgcaggata tcctgggcgg ggaagtatat cgagacgggc tgacgcatgt
    16921 gctgtctgaa cccgagttcg ctgaggtgga tgtggcccgc aaagcgctgc gcattttaga
    16981 agagcgttcg ctcctcgaag atctgcttgc ccgcacgatc atgaatgacg ccattggcgg
    17041 tgtgcaggtt ctcattggcg gcgagggcac ctgggaggaa ctacgtgact gctcaatggt
    17101 gctagctcgg tatggcgtgc caggtctagc cacgggcacc ctgggcgtgc tggggccgat
    17161 ccgcatggct tacggtcaga ccatctccac cgtgcgctat gtggccgggc ttttgagcga
    17221 tctggttgtc gatttacacg ccgaataatc gagcatcaca gttttttgat gagtttttga
    17281 tcgttatcag aaatttacct gtttgcagga caaggagcaa tgacaaacga aagtatcagc
    17341 caatcagaga accacgaaat ggatcaggta gcctccggag aagcacaaga aaccgtcgag
    17401 gcgcttcaag cgaccacacc agacgacgtt caggtattaa aggacgaact catcgaatgg
    17461 caaaccaaag ccaatgaata cctggatgga tggcagcgcg cccgggcgga ttacgccaac
    17521 tacaagaaac gcgtggaacg tgaccaagcc caggcacacc aaaacgcaac ggcaaatgcc
    17581 atccgccgtt tcctggaagt gaaagacgat ctggagctgg cactcaaaaa tcgcccaaac
    17641 cagggcgatg atgccggttg ggcagacggg gtggagttga tctatcgcaa gcttgctgcc
    17701 ttcctcgaag ccgagggtgt gagcatcatc gagacagatg gcaaattttt cgatccaaac
    17761 ttccacgaag ccatcactca cgaggtccat cccgagctgg agagtggtca aattatcgct
    17821 gtgcttaaac aaggctatcg tatgggtgac cgggtgttgc gccccgccca ggttcgggta
    17881 gctagctaat cggaggaatt cattatggga aaaattattg gaatcgattt aggtactacc
    17941 aactcagtcg tggctgtcat ggaaggcggg gagccggtgg taatcccctc ggcggagggc
    18001 gagcgcctgg tgccgtctgt cgtcgccttt aacaagaacc gggagcgcct gatcgggcgc
    18061 gtggcgcgca accaggctgt cgtaaacccc gagaacacga tcttctcgat caagcgcttc
    18121 atgggacgca agtttagcga tcccgaggtg cagcgcgcca cgacacgcgt gccatataag
    18181 gtagcacagg cgcccaatgg cgatgtgcgc gtgatccttg acgagcgcga atattctccg
    18241 cccgaagtga gcgccatgat cctgggcaaa cttaaggcag atgctgaggc ctacctgggc
    18301 gagacggtca cccaggcagt gatcaccgtg ccggcttact tcaacgacgc ccagcgcaat
    18361 gccaccaagg acgcaggcaa gatcgccggg ctggaggtgc tgcgtatcat caacgagccg
    18421 accgcctcct ccctggccta tggcctggac aagaaaaagg acgagctcat cgccgtctat
    18481 gacctgggtg gcggcacctt cgatatctcc atcttggacg tgggagacgg ggtcttccag
    18541 gtgcgcgcga ccagcggcga caccttcctg ggcggcgacg atttcgacca gcgcatcatt
    18601 gattttatag ccgacgagtt taagcgcgaa caaggcattg acctgcgcaa agataggcaa
    18661 gccctgcaac gcttgaaaga gggggctgag aaagctaaaa tcgagctttc cagcatgatg
    18721 cagaccgaga tcaacctccc atacatcacc gcagatgcca gtggaccgaa gcatctggtc
    18781 atgacgctga ctcgcgccaa gctagaacag ttgactgccg acttgatcga acgctcgctt
    18841 gggccagtgc gacaggcgat gaaagacgcc gggctagaac cagaccaggt gaacgaggtc
    18901 gtattggtgg gcggcatgac gcgtatgcct gctgtgcagg acgcggttcg caaactgttc
    18961 ggtaaaggcc cccataaggg tgtcaacccc gacgaagtgg ttgcggtagg tgcagcgatc
    19021 caggcaggcg tgctgggcgg cgaggtgaaa gatatcttgc tgctggacgt cacgcccctg
    19081 accctatctg tagagacctt gggcagcgta gccagcccga tgattgagcg aaacaccacc
    19141 atccccacgc ggaaaagcca gattttttcc acagctgccg acagccaacc tcaggtggag
    19201 attcatgtac tgcagggcga acgccccatg gcaaacgata acaaatcgct gggtaagttc
    19261 atcctggatg gtattccacc cgcgccgcgc ggcatcccgc agattgaggt gaccttcgac
    19321 atcgacgcca acggcattct caaagtcacc gcacaggaca aggctaccag tcgcagccag
    19381 aacatcacga ttaccgcctc atcggggctc gccgaagcag aagttgagcg catgcgtaag
    19441 gacgccgaga tgcacgctga tgaggatcgc aagcggaaag agttgattga ggcgcataac
    19501 acggccgaca acgccatcta cactgccgaa aagtcgctcc gcgacctggg cgataaaatc
    19561 cccgcagaac tcaagagcca ggtggaagag catgtttcca aagtgcgcga ggtgattaac
    19621 agcgaagacg cggagaaaat tcgccaagaa accgaggcgt tgttcagcgt ggtgcagcag
    19681 gtcggcaccg ccgcctacca gcaggaagcg cccagcgagc aggcgcccag cgaagatgaa
    19741 caaggcgacg agccagattc gggacctgat gacgaagaag ttgtcgatgg tgagtttaga
    19801 aacgtttaat gcttattggt tcaatgacaa caggttggag gctagcaggc tgctcgtatc
    19861 tgcctgcatc cggcaaccat aatccatgat gactgaacga gattattacg aaatcctggg
    19921 cgtcccgaag aacgcttcca atgaagagct gaagtccgct ttccggcgtt tggctcggca
    19981 attccatcct gacgtcaaca aggaaagcga cgccgaggaa cgctttaaag agatcaacga
    20041 ggcgtatgcc gtgctatccg accaggataa acgctcggtt tacgatcgct taggtcacgc
    20101 cggagtgaag ggagccggcg gagtacctga tttcagcgtg gatttctcgg atttcgccga
    20161 tatcttcgga gatttatttg gctttggtgg cttcacacgc agctcgcgcc gccagcgcaa
    20221 cgcaccccgc cgcggacaag acctctcgta ccggcttgat ctgacctttg aggaagccgt
    20281 atttggtctg gagaaagaga tcgagtttgt ccgcgatgag ttgtgcaaca cctgttctgg
    20341 ctctggcgcg gagccaggca cttccccggt tcgttgtacg acctgcaacg gctcaggcga
    20401 gatgcgccag gttcgccaga ccctgctcgg gtccatggtt caggtatcta cctgtcccac
    20461 ctgcaaaggt gtcggcgaaa ccatcaccac accctgccat acttgccagg gtcgcggcct
    20521 ggagcggcga acccgtcgca aggtcgtgaa catcccgggc ggagtggatg acggtaccca
    20581 aatccgcctg gctggggaag ggcagccggg cttgaacggc gggccgaacg gcaaccttta
    20641 catcaccatc catgtccagc ctcacaaatt cttccgccgc agggagaacg atatcatcct
    20701 tgacctggac attaacctgg cgcaggcgac cttgggagca gaggtggaaa tacccactgt
    20761 agatggccca gcgcgattga agatcccctc cggtacgcag cctggcaagg tactgcgtat
    20821 gcgtagcaaa ggagtgccct acctgcgcag caacggtcgt ggagaccagt tagtggtggt
    20881 taatgtagat attccgaccc gcttgaccag tgaacagcgc gagctttttg agcaactggc
    20941 aaaaagcctg gggagcgaag tgcgcccgca agagcgcagc tttattgact ggttgaaaga
    21001 aaccttggga gggtagttaa tgccctcgag tcgccccgac ttacacttca ctcacctcta
    21061 accccctttg gagcggggga aagatcggaa tacaggctga atgggtaatt caagctggct
    21121 tgagatatct ttgattgtcg atggagagat ggctgaggcc gtagcggagg tgctagggcg
    21181 ctatgtctcc ggcggtgtcg ccatcgagtc tacggcagtg acagccaggc cagacgacag
    21241 cgaagggcag gctatcggtc cgctgcgcgt gtgcggttac cttcctgttg acgaccagct
    21301 tgacgagaac cgccagcgtg tcgagcaggc tttatggttc ctggggcgta tccgtccttt
    21361 gccagcccca caataccaca tcatacacga acaagactgg tcacaagcct ggaagtctca
    21421 ttaccacccc atcgccatcg gtcagcggtt gatggtcgtg ccagcctggg agcagcctcc
    21481 cgatccagtg cgcatcgcca ttcgcatgga ccctggcatg gcttttggca ctggcacaca
    21541 ccccactaca cagctctgcc tggagctgat ggaaatttat cttcccggaa gggaagccgc
    21601 aattatcgac ctgggctgcg gatcgggcat ccttgccgtg gcggcgctca aacttggcgc
    21661 ggaacaagcc ctgggcgtgg atatcgactt ggaagccgtg caggcgacgc gcgataacgc
    21721 cgaactgaac ggcgtggcgg gtcagctgga aattggcaag ggttctgttg acgaaatccg
    21781 ccggggtgaa tttgcgcttc gccgcgcaaa cctggtgctg gccaatatcc tggcacctgt
    21841 gatcattcgc ttgctggatg atggcctgcc cgatctgctc acacccgacg gcgtcctgat
    21901 cctttcaggc atcctggaag agcaagcggt tgaggtcgaa gacgccctgc aaagatacaa
    21961 cctgcgcacc ctggatcgcc gccagtccgg ggattgggta gcgctagttg cggggatgat
    22021 attataataa atacatgaat gagaacacct tcccaccttc tgcccccaaa cccaatccgg
    22081 taacccagcg caagcatcgc caggaggttc tctggcaaat tacgctgcca ttagcgctcg
    22141 gcgcgctgct ggtgctggct gcgggggctg gtgtggttta cgctggagcg acccagacag
    22201 gcccggtgga tcgttgggcg agcatctcca tcatatggct gatcatcccg accatgtttt
    22261 taaccctcat ctttctggta gtgactgcgg gtctggcgta tggaatcata tggctaattg
    22321 gcgccctgcc taaatatacc cgccaaatcc aggatgtatt tgttttgatt gatgcgcggg
    22381 tgaggaaagc cgctgatgct atcgtggagc cgaccttacg tgttcaaagc ttcacagcag
    22441 ggctgcgggc gctgcgacgc aagtgagcaa aggagtgatt aatgtcaaac aatggaaatg
    22501 aacaggctgg cggaaactgg aaatccaagg ctctgctagc tggggcagtt ttaggcgcag
    22561 tggtaggtct tagcgctgcc tacctcatca ttcagcgagc tgagcgcgaa gatggggagg
    22621 tgcgcatctc cccaggtgat ggggtgaaac taggagtgct ggtgttcggc ttgctgcgtc
    22681 aagtggccca gctgggtgag ggcgagaaat aacccaggat aaatatccaa agattacaga
    22741 tcggggcgga ctgaccgccc cctttttatt ggtaggattt tgcaggagat tatcagatgg
    22801 aagaattaaa agaaatgaaa tgtgtgcctt gccgcggcgg agtgccccct ctaaccagtg
    22861 aacagattgc cggcttgcat ccgggtgtcc cagaatggca ggtgctcgat gtggaggata
    22921 tccaaaggtt gcagcgggta tttaaattca agaattttgc ccaagccttg gaattcacga
    22981 acaaagttgg cgcgctagct gaagaagaag atcaccaccc ggcgctgctc accgagtggg
    23041 gcaaggtgac cgtgacatgg tggactcaca agatcaaagg cttacacaat aacgatttca
    23101 tcatggccgc caagacggat gccttagtac agtaaatcca tataactggc aaaacatcgc
    23161 catataagct taccttgcga gtgcttgaga tgacgggctg tgactcttgg ggtggatgtg
    23221 cgccctgtgt gtaggggtac ctcgcaataa ctttttcaaa gaaccctcga accctcattt
    23281 gatagctacg ctcattgcaa gctcttgaca ggcgaactat ctctatctat aatatgaacg
    23341 aacgttcgtt cagtatagag cgagaaactc ctatgtctga agagatcgcg cctcgcgggg
    23401 agcgcacccg caacgacatc cttcaggccg cctaccaatt gttcttggag cgcggtttcc
    23461 atggcacgtc tatgcgccag atcgctcaag cagccggtat cgctctgggt ggcatataca
    23521 accatttctc cagcaaggaa gacatattca cagcagtctt cctagaacgc catccctttc
    23581 gggatgtact gcctctgatg aacaccgcgc agggcgagac ggtagaagaa tttgtgcgcg
    23641 atgctgccca gcgcatggtg gccggtttag ggcaacgcac cgatttcctc aacctgatgt
    23701 tcatcgagct ggtggaattt aaggggcagc atgtcccaca catctttcaa atcatcttcc
    23761 ccgaaatgat ggagtttacc cagcgtttcc agaccgagcg ccaggagctg cgcgatatcc
    23821 cgcttttggt ggtggtgcgc gccttcatcg gactcttctt ctctctcctg atcacagaga
    23881 tcatgatcgc agaccagttg ccgccagagg agcgcgagca tgccttggat tatttcgtcg
    23941 atatttacct atacggaatc ttgaagggtg gcactacact cgagaaggtg caacaatgaa
    24001 ctctcgtctg acctccctga tccgcaaaga gtttctccag atcttgcgcg acccgcgcac
    24061 cctagcgctg gcgctggtca tgccattagt gcaacttttc ctgctcggct attcagctac
    24121 caacgatgta cgcaacgtgc cgctggcggt ctacaaccag gatcgtgggc ctgaagcgcg
    24181 cgccttgctg gacgcttacc gcgccgccaa ttacttttct ctggagtacg atgtgagctc
    24241 cgagcaggaa ctgcgcgact tgatcgacag aggccaggcg cgcggcggcc tggtcatccc
    24301 accagattac agctcccgct tacagggaga tggccaagca gaggtggcat ttatcttgga
    24361 cggcagcgac cccacggtgg cagccacggc actctcggca gcgcaggtga tcggccagca
    24421 gcatgctacc agtgtgctca gcgaccgcct gggtcggcgc gggcagtcct ccgccatccg
    24481 cccgcccatc gaagtgcgca cccaggtttg gtacaaccca gacttggaga gtgcttactt
    24541 tatgatcccc ggagtgattg ggatgatcct gtacgccatc acctctatcc tgacttccac
    24601 agccatcgtg cgcgaacgcg agcgcggcac catcgagcag ctcatcgtca cccctattcg
    24661 cccctgggag ctggtggtgg gcaagatgtt gccctacgtc attttggcct ttcttaatac
    24721 gctggaagtg ctcgccattg gcgctttgtg gtttcatgtg cctatccgcg gcgacctggg
    24781 tctgattttg ctgctctccg cgttgttcct actcaccagt ctggggatcg gcctcttagc
    24841 ctctaccatc gctaacacgc agcaggaggc catgctcacc gtatggatga cgctactgcc
    24901 cagcatcttc ctctccgggt tcttcttccc gctggaggcc atgcccaaag tgctccagtg
    24961 gctttcttac ctgatgccgc tgcgctatta tctggtgatc atccgctcgc tgctgctcaa
    25021 gggtgtgggg gcggacatgc tcaagagcga tatcctggcg cttgcaattt tcggttttgc
    25081 cattatggca gccgcggcga tgaggttccg gaaaagactg gactgatcat cgtaggaggt
    25141 tcccatgcat ccaaatccta gaaagatagt gcctattgtt ttgctgatcg ccatccttgg
    25201 gggtgcgatc tggtatttta ccctcgggca ggggaacggc aattccagtg cgttaaccgc
    25261 ctcagggacc atcgagggca cacaggtggt catcgcccct gaacttggcg ggcgtgtacg
    25321 ggaagttatg gtccaggagg gtgaggtggt ggaaaccggg cagaccctag tgcgcttcga
    25381 cgactccctg ctgcaggcgc agttaagtca agcacgggct gcattgcatt tgacccaggc
    25441 caactacgat ttagccgcca aaggaccgac agatgagcag cgccgacttg ccatcgcctc
    25501 agcggagatg gagctgacga ctgcccggca ggcactgcaa gccctgtacg ataattcagg
    25561 cttgacggaa tcacagctcg agcagtccat cgctgccatt gataaatccc gcgataaggc
    25621 gcagcaatac ttggacaatg tcaataccga ggctgacccc tcagacatcg acgccgcatg
    25681 ggcttcggtg gttattgccc aggataagct ggaggacgcc ttagaggact tcgagccgta
    25741 tgagaaaaaa gatcagagca acctgacacg cgccatcttt caggcacagg tggcctctgc
    25801 acagaagcag tacgattcca tggtcgagcg ttacaacaat ctggtcggca ccgccaacca
    25861 gtatgaacta gccttggctc gttcggaggt aagccttgca gaagctaagc tggccgatgc
    25921 aaaacggcaa tacgaagaat tacaggaagg catcgatcct gactccctgg ccctggccca
    25981 ggcgcatctg aacactgccg aagccaacct ggcggttgcc aaagccgacc ccagccccga
    26041 gcaactggcg gctgcccagg cacaggtgga agtcgcgcag ggtgcgatgg aagtgattga
    26101 agcgcagatc gccaaactgg tcatcaacgc tccaatggat ggggtagtgc tgacccgcac
    26161 ggcggaaccg ggcgaggtgc tggttccggg cgcacccctg ctatctctac tcagattgga
    26221 tgatcttact ataaccgtat atgtacccga agaccgctat ggcaccatca agctgggaca
    26281 gaccgcttcg gtgacagtgg actcgtatcc aagcgaggcc tttgaggcca tggtcatacg
    26341 catcgctgac caggccgagt tcaccccgcg caacgtgcaa accgaagccg gtcgccgtac
    26401 aaccgtgttt gcagtggaac tctcggtgac aaatccggag ggcaagctca agccgggcat
    26461 gcccgcggat gtagacttta gtctcaagtg aaagtgttcg gcaatttcac cactttcacc
    26521 cccatctcta tggacatact tattgaagcc cttaatttgc ataagtcgtt tggcgaaacg
    26581 cgggctgtgg atgggttaaa cctgcgcgtg gcggcagggg agatctatgg tctggttgga
    26641 ccggacggcg ctggcaaaac caccacgcta cgcctgctgt gcgcggcgct gcaacttgac
    26701 cgaatcaaag acgacgagca atcctccatc cggatcagtg gatatgatgc agtccgccag
    26761 actgagctcg tccgcgctca gatcggctac ttgccccagc gcttttcgct atatgaagac
    26821 ctgaccgtgc tggagaacat ccgcttcttc gccgaggtgc gcggcatgag ctcggcggat
    26881 tggcgaccgc gctgtatgga aatcctgggc ttcgtcgggc tggatccgtt tgtcgaccgg
    26941 ctggcaggtc atctttcagg gggcatgaaa cagaaacttg gactggcagc agcgctggtg
    27001 cacaggccgc gcgtgctgct actagatgag cccactaccg gggtagaccc agtcacccgc
    27061 caggatttct ggcagttgat catccgcctg gcaagccagg caaaccacga tcacgccgag
    27121 gtggccgtgc tggtcagcac cccgtatatg gacgaggcgg cgcgttgcac gcgggtgggc
    27181 ttcatgcgcg cgggtcgttt gctgatcgaa ggctcgcctg ccgatctgcg cctgcgcctg
    27241 aaggatcaaa tcctggagct acgtggccaa cccttagata tgctgcgcca ccttgcacac
    27301 gctgaccagg atgtcgaaga tgtacagatg ttcggcgacc gcattcacct gcggacccgg
    27361 ctgggctcga gggatttggt cgaagagcgg ctgacacagc gcatccttgc cgaaggcggc
    27421 gaaatcaccc tgctccgtcc agttgcgccc cagctggagg atgtgttcat ttctttgttg
    27481 gatcaaccta attcatcatc ctgaaataaa ggtctcattg ggaatcgccg aggaaaaaca
    27541 gagataaaag cgcaagattg agtatgagta tagaagtcat ccatgcaacg aatctaactc
    27601 gccgctttgg cgatttcacc gcagtggacc acatctcttt caccatgaac gccggtgagg
    27661 tcgtgggcta tctggggcct aatggttcgg gcaagaccac cactatccgc atgttgctgg
    27721 gcctgctcat ccccaccgag ggagaggcca gcgtgctcgg ctacgacgtg gttcgccagg
    27781 ctgagcagat acgggagcgc gtggggtaca tgtcgcagaa attcgccctc taccacgacc
    27841 tcactgtagg cgaaaatctg gccttctacg ccggggtgta cggcgagcgc tcgccggcac
    27901 gcctggcaga agtgctggag ctggtggggc tcagccagct cgaaggggag cgggtaagcg
    27961 acttgccggt tggctggcga cagcgcctgg ctttgggcac agccatcgtt caccgcccca
    28021 agctgctatt tctggacgag cccaccagcg gagtagaccc tacgactcgc cgcgcttttt
    28081 gggatttgat ctatgcgctg gtggacgaag gcgtgacggc tttggtgacc acgcactaca
    28141 tggatgaggc ggagtactgc ggacgagtcg gcatcatgcg cgacggaaag ctactagcta
    28201 tggatgctcc atcgacacta aaaaccagcg ccctaccagg gctgacatgg gatgtgttct
    28261 tggatggggt cagtagcacg ggggcgtcat cactgctagt tgccctaaat gccctggaga
    28321 catgcccgtg cgtattacgg gcggggttgg tcagcgatca cctgcgggca atcacgcccg
    28381 ccgatgtgag tgctgagaca ctgcgccaac atctggatga ggcgggcatc gaaggcttac
    28441 gcatagatcc agccgagcca accctcgaag atgttttcct ggctttagcc accagataac
    28501 catctcttta ttgcccctgc tacgccaaat tcccaagacc caaactttta tatcttttct
    28561 ttctaacgta taattcttaa cggattattc gtcatatgga atcaaaattg cctcgcttca
    28621 tcatcgccgg atcgttgctt cgcgaatatg tcatcctgcc ttcgggcgag gcactcctgg
    28681 acgtacccgg tgggaacgtt ctctacgctg ctgttggatt gaaattatgg gaaccggaac
    28741 cccctcccgc cgttctggcc cgtgtgggcg aggactaccc ccaggagtgg ctggatcagt
    28801 ttacccggcg agggctagat acacgcggtg tgaacgtcat ctcccagccg gtggatctgc
    28861 gtgcttttac cgcttttctc agccgcacaa accgtgctac cgatgacccg gttgcgcact
    28921 tcgcccgtgc tggacttacc ttcccgaaag aactgctggg gtaccgtgat tcatcgtctg
    28981 accttaacag ccgcacgaat ctatcatcca tctcactgcg ccaaggtgat atccccgccg
    29041 actacctgga cgccagcgcc gctcacctat gccccctgga ttacctgacg cacagcctgt
    29101 tgcccgcagt tctgcgccaa gcggaattca ctaccgtcac cctggatcct tcgccaggtt
    29161 acatgaaccc tactttctgg gatgacgtac cagcgctggt gaccgggttg acggccttcc
    29221 taccctccga agaagagatg agctccctat tccaagggcg cagcaccgac ttgtggcaaa
    29281 tggccgaggg cctggcttcg tacggttgcg agattattgt gatcaagcgc ggcgagggtg
    29341 gccaattgct ctacgacgca gctgggcgcg cccgatggga gattccggcc tatccctctc
    29401 gcatggtaga tcccagcggc gcaggcgacg ccttctgcgg aggcttcctg gcaggctacc
    29461 ggcgcactta cgatcctctc caggcagccc tgcatggcaa catctcagct tcgctggtag
    29521 ttgaagggaa tggtcctttt tatgccctgg actgcctgcc tgcgctgcca caagccaggc
    29581 tggaggcttt acagcaagga gttaggaaaa tatgagttcc gtgttaggtc ttgcgctgag
    29641 cgagcgcgtg ctggctctgg ctatgaccat ccagcagatc cctgcgccca cattctccga
    29701 ggctcggcga gccgctttca tctttgagcg ttttcaagcg gaggggctga gcgacatctc
    29761 aatggatgca ataggtaacg tgtatggcag gttgccgggc gcagggagca cgccgccgct
    29821 ggtggtcagc gcccatctcg acactgtctt tccggaagat acgcctttag actgccgccg
    29881 cgaggcggag acgatctacg gtcccggcat tggtgataac gccctgggtg tggcagggtt
    29941 gttcggtctg ctttggggcg taaaagaaat tgtctcaccc aggcttcagc ccaacctctc
    30001 aggtccgaaa gcgaaatctt cgcttgcctt acccggggac ctgtggctgg ttgccaatgt
    30061 ggccgaagaa ggcatgggca acctgctcgg catgaccgcc gtggtagacc gcttcggggg
    30121 tgatgcgcgc gcctatcttg tcctggaggg gatggcctta gggcaagtat atcatcgcgg
    30181 tctcaatgta cggcgctacc agattagcgc cagtacagct ggcgggcact cttgggtgga
    30241 ttatgggcag ccctccgcca tccacgagct ggcggggctg atcacccgcc tttgtgacct
    30301 atcgctgccc agccagccac gcaccaccct gaacgtgggt gtaatttcag gtgggacgac
    30361 ggtcaactcc atcgccgcgg aggcaaactg cctgttagac ttgcgctccg agggacacga
    30421 aagcatggtt aatctagcca ccgaagtgga aggactgatc gacgccggca atcgcagcgg
    30481 cgtgcagttc tcttgggagg tggtcggcca acgccctgcg ggagagatgc cacgagatca
    30541 cccgctggtg caactggcgg cgagctgcct ggaatcgcaa ggcatccagc cgctgttcaa
    30601 tatcggttca accgacgcca acctgccgct cagccgcg
//
LOCUS       DBNGSX010000030         3575 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1664124, whole genome shotgun sequence.
ACCESSION   DBNGSX010000030 DBNGSX010000000
VERSION     DBNGSX010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1664124"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="AC16CB_01110"
     CDS             complement(<1..817)
                     /locus_tag="AC16CB_01110"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR3341092.1"
                     /translation="MSERSLQPDTDLVYDLFYGVFKPNLIRLALQLDIFTPLADGPAT
                     AQAVAMACACQVEGIQAMLEYLCSLLVLDQSGDKFSLTPTAGTFFIRGRKSYVGDLIL
                     HDTGPAIYERVRGALTSGVPSALGIDFVQDAWLESYRTSRLSSSLEMWGAAGIHPGPE
                     LDLRLLDIACGCAIKSLSLAQLSPRMRLTCLDTEQVLEVARDLAERMHAASQVTYLPG
                     SLHEINLGEDCYEAALLGQISHYLTVSEVQALFRRICAALTSGGKLVIDCPMGG"
     gene            complement(833..1279)
                     /locus_tag="AC16CB_01115"
     CDS             complement(833..1279)
                     /locus_tag="AC16CB_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HIR3341093.1"
                     /translation="MLDASTSALEIDFRWQINMFNAWRGWARSLKRNTYALYLAARHP
                     GVPVLAKILTALVAAYALSPIDLIPDFIPVLGYLDDMLLLPLGIALAIKLVPGPIWEE
                     CRQQADRELSSPLPRSRTAAAVIVAIWLLAISITLSVLWRTVIQYG"
     gene            complement(1309..2064)
                     /locus_tag="AC16CB_01120"
     CDS             complement(1309..2064)
                     /locus_tag="AC16CB_01120"
                     /inference="COORDINATES: protein motif:HMM:NF022441.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HIR3341094.1"
                     /translation="MFTKQSFPIKLLVMSMVSVVLIASCTNVLGFNRRVSGSGNVVQE
                     NRTLQSFTEVVLSEEGDLFIDVGDREQLIIEAEDNLQEYLVAEVNIGILEIKKLPENV
                     TLNATQPIHYYLTVKELKSLTIKNSGDAEVSEVRADKFLVHVTSSGSVHIGKLDVQRL
                     DVELTSSGDLVIDEGEADEQFIRLSSSGEYIGKNVICQSAQVRLSSSGNATLNVLEVL
                     EADLSSSGNVYYIGNPRIVYKDSSSSGRVQPLP"
     gene            complement(2950..3540)
                     /locus_tag="AC16CB_01125"
     CDS             complement(2950..3540)
                     /locus_tag="AC16CB_01125"
                     /inference="COORDINATES: protein motif:HMM:NF021221.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnetosome magnetite crystal formation protein
                     family"
                     /protein_id="HIR3341095.1"
                     /translation="MNQPDLGLKVAELRQQKALTQEQLAEKCEVSTRTIQRIERGEVD
                     PRAYTLQCLGEALDFDFGEDTSQSESFWLVVMHLSSILPTLIIPLLIWSWKKRQSYKI
                     NEQGRDVLNFQITMMLLLFLGGFLLTFLPVALPSLGKALDDMGVNMVLFQFMVLCATA
                     PMVLIGIFCTYQGVVNAMRALLDKPVRYQLSLPFVK"
BASE COUNT          923 a          946 c          841 g          865 t
ORIGIN      
        1 ctccacccat tgggcaatcg atgaccagct tgcccccaga ggtcaacgca gcacagattc
       61 tacggaacaa cgcctggact tctgatacgg tcaggtaatg cgagatctga cccaggaggg
      121 cagcctcata gcaatcctcg cccaggttga tttcgtggag actccctggc aggtaagtga
      181 cctgcgaagc ggcgtgcatg cgctccgcca ggtcacgggc tacttccaag acttgttccg
      241 tatccagaca ggttaagcgc atacgtggag agagctgggc tagcgataag ctcttgatgg
      301 cgcacccgca ggcgatatcc agcagccgca agtccagctc tggcccaggg tggatcccgg
      361 cagctcccca catctccagg ctacttgata agcgacttgt cctgtagctc tccagccagg
      421 catcctggac gaaatcaatc cccagagcag aggggacgcc gctcgtcagc gccccgcgca
      481 cgcgctcgta gatagccgga ccggtgtcgt gcaggatcaa atcgccgaca tatgattttc
      541 gtccacgaat aaaaaaggtc cccgcagtgg gcgtcaggct aaatttatcc ccagattgat
      601 ccaggacaag caggctgcac aggtattcca acatcgcctg gatgccttcc acttggcaag
      661 cgcaggccat ggctaccgct tgggctgtag ctgggccatc cgccaggggg gtgaaaatat
      721 cgagctgcaa ggccaggcgg atcaggttgg gcttgaacac gccataaaat agatcatata
      781 ccaggtcggt atcgggttgg agcgaacgtt cggacatcct ctttctcctt cgttatccgt
      841 actgaataac ggtccgccac aagacagaaa gcgtgatgct gatcgccagc agccagatgg
      901 cgacgatcac ggccgcggct gtcctgctgc gaggcagtgg gctggatagc tccctatcag
      961 cctgttggcg gcactcctcc cagatcggtc ctggcaccag cttgatcgcc agggcgatac
     1021 ctaagggcag caaaagcata tcatccaggt agcccagcac ggggataaaa tccggaatca
     1081 ggtcgattgg gctgagggcg taggcggcta ccagcgccgt taatatcttt gcaaggactg
     1141 gcacgccagg atggcgggca gccaggtaaa gagcataggt atttcgcttt agagagcggg
     1201 cccatccccg ccaggcgttg aacatgttga tttgccagcg gaaatctatc tcaagcgcgg
     1261 aggtcgatgc atccagcaaa ccattctcgc ccaggtctgc tctagtacct acggcaaggg
     1321 ttgaaccctg cctgaagatg aagaatcctt gtagacaatt cttggattac caatatagta
     1381 cacattcccg ctactggata ggtctgcttc cagcacttcc agcacattga gagtagcatt
     1441 cccgctactg ctcaatctta cctgagcgct ttggcagatt acgtttttac caatatactc
     1501 accagagctg cttaatctga taaattgttc atctgcctcc ccctcatcga tgaccaaatc
     1561 cccggaacta gtcaattcca catccagacg ttggacgtcc agcttgccga tgtgtacgct
     1621 tccagaactt gtcacatgaa caaggaattt atcggctctc acttcagaga cctcagcatc
     1681 accgctgttc tttatggtta aagacttgag ttcttttacc gtgaggtagt agtgtatggg
     1741 ttgcgtcgca ttcaaggtga cgttttcagg aagctttttg atctctaaga tgcctatatt
     1801 tacctcggcg accaggtatt cttgcaaatt atcttctgct tcaataatta actgctcccg
     1861 gtctccgaca tcgatgaaca gatctccttc ttctgagaga accacttctg taaagctctg
     1921 cagcgttcta ttctcttgaa ccacatttcc tgaaccagag actctgcgat taaaccccaa
     1981 tacattggta caactcgcaa ttagaacgac actaaccatg ctcatgacga gcaatttgat
     2041 tgggaacgat tgttttgtaa acatgattat cctttctagc tcttgatagc tcccctcaaa
     2101 ctgtcaaggg agcaaaatcg aatgcagaag atcattcata aaaaatccaa aatcaaatgc
     2161 cagtatgacc aatcccacat tgagagccaa atggcaaatc cgtggccaat tggtcatgtt
     2221 aacgtgcccg tccaaccacc tgaatatcac tgctaggatg ccaaacagag aaaatgtgaa
     2281 aggaaggggg aaatcccagc gccagaacaa caagctaatg acaaggttat gtaatagacc
     2341 gttcaccaaa aaaacagcca gtgttgcggt attacgtctt tttttcccgc caaactttaa
     2401 gtacagtcta taggtgaaat aaccgatacc cggattccat atctgccaga agcgatgaaa
     2461 gccgggctcc agccagcact cgagaaaccc tcgcctcacg aaaacccagt catttacatg
     2521 atttccaaag ccacgcagtt tcaagtattg cttgtaattc aaaacatatt ggttataaga
     2581 gcgaatcact cttcctttta tggcccaaat atcataggaa cccgacaggc tactcttcat
     2641 tattctccaa ttgcatagat attttggtat tcgtcatctt tgcaaatatt gaagtgacga
     2701 gtccaatcgc ctgtagaggc gcggtaaata cttgtggaat gatccgtaga tcgaggccaa
     2761 ccggagaatc aacgaccttt atgttatctc gggttgtaat ctccacatgg cggatagttc
     2821 ggtcccttgc acccttaata gatctccaga tgcctttaga aggaaattcg atgaggtttt
     2881 tgctagacat atttctcctc cgtattactt atgctttact atttcatgca gcaagactcg
     2941 ctacaaatat catttgacga atggaaggct aagctggtag cggactggct tatctaacag
     3001 cgcgcgcatg gcgttgacca cgccctggta agtacagaat attccgatca ataccattgg
     3061 cgcggtggcg cacaacacca taaattggaa cagcaccata ttcaccccca tgtcgtccag
     3121 tgcttttccc aaggatggaa gagcaacagg taaaaacgtg agcaggaagc cgcccagaaa
     3181 caacaatagc atcatggtga tctgaaaatt gagcacatca cgcccctgtt cattaatttt
     3241 atagctctgt cttttcttcc aggaccatat gagcagtggg atgatgaggg tcggcaggat
     3301 gctactcagg tgcatgacca caagccaaaa cgattcgctt tgggatgtat cctccccaaa
     3361 atcaaaatcg agcgcctcgc ccaagcactg tagggtgtaa gcgcgtggat ccacttcgcc
     3421 acgctcaatg cgctggatgg tgcgcgtact gacttcacat ttttcagcca gctgctcctg
     3481 ggtgagtgcc ttttgctggc gtagttcagc tactttgagt ccaagatctg gttggttcat
     3541 ggcgtactcc tttcgctatc tccccattat tatag
//
LOCUS       DBNGSX010000031         2953 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_971572, whole genome shotgun sequence.
ACCESSION   DBNGSX010000031 DBNGSX010000000
VERSION     DBNGSX010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_971572"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="AC16CB_01130"
     CDS             <1..519
                     /locus_tag="AC16CB_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HIR3341096.1"
                     /translation="SAQVLLPLDELNLLLIEQPLFEDDLWDHSRLQAQFTTPICLDES
                     IISPRHARQALEMGACKVINIKAGRVGGLSQALAIHDLCRSRGVPVWCGGMLETGVGR
                     AYNLALASLPGFALPGDISASDRYYTQDITQERFKLNPDSTIDVPDAPGLGVSIDQDA
                     LNKFTIKKDLLK"
     gene            complement(575..2689)
                     /locus_tag="AC16CB_01135"
     CDS             complement(575..2689)
                     /locus_tag="AC16CB_01135"
                     /inference="COORDINATES: protein motif:HMM:NF014628.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HIR3341097.1"
                     /translation="MADTAFRDHIRSALADENLQVALHANAVRRLNARQQAFSSLPRP
                     WEEMRQRAHAVRAVVIANLDELLAKFIERAEMNGMTVLRAADGTQAANTILEIARQNG
                     AQLVAKAKSMVSEEIGLNQILESAGLRVVETDLGEYIVQLRGEHPAHIITPAVHLRRE
                     QVGQTFYEKLGIPYTVDIPTLTLAARRVLRQVFLDAQIGLSGVNFGVVDSGTLCVVTN
                     EGNGRMVTSLPKVHIALMGIERLVPSLDDLALMLYLLPRSATGQKLSVYTNLINGPAH
                     LDAPSGPQQRYLILLDNGRTNLRQSPLVEALYCIRCGACLNACPVFREIGGHAYVNPS
                     GKSSPYSGPIGSVISQGLFGQDFGNLARASSLCGACEDVCPVGIDLPKLLLRVRAGDV
                     QPLKPPPMEDKPGESDNKTPISNAPGWLAFGIRMYTWFATSASRFSLAQRLASFFGYL
                     ASPVSMWMRFPAITGWGYSKDFPRPALKPFRARWREIRENDYSASRHSVNGESTELPP
                     NVGQQSSDQPAIDEQAQALARLVQFSNEISAVGGEFVRCREEELSKHVLDLLQDRDIR
                     SIQAWEEGYLPTGLLEELRAAGIAIHHEPDPEVRGGLTGASAAIAETGSLLVCEGGGR
                     PLTASLLPEVHIAVIQEHDIYENLTQVLNLQEVRQATSAVLITGPSRTADIEMTLTIG
                     VHGPKELVVVCMTPQEVAIHRE"
     gene            complement(2714..>2953)
                     /locus_tag="AC16CB_01140"
     CDS             complement(2714..>2953)
                     /locus_tag="AC16CB_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006839527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HIR3341098.1"
                     /translation="VELRETAECCGFGGVFSLEHPELSAAMLERKIANIETSGADWVV
                     SCDGGCITHINGGLNRSGKPRRAIHIAQILDRSDL"
BASE COUNT          669 a          868 c          765 g          651 t
ORIGIN      
        1 agcgcccaag ttttgctgcc cttggatgag ctaaacttgc tgctgatcga gcagccgctt
       61 ttcgaggacg atctctggga tcacagtcgc cttcaggcgc aattcactac ccccatttgt
      121 ctggacgaga gcatcataag ccctaggcac gcccgccagg ccctggagat gggtgcctgt
      181 aaggtgatta atatcaaagc cggacgcgtg ggcgggttga gccaagcact tgccatacac
      241 gatctttgtc gctcacgtgg cgtgccagtc tggtgtggcg gcatgctgga aaccggtgtg
      301 gggcgcgctt acaacttggc tctggcatcc ttacctggct tcgctctgcc aggagatatc
      361 tccgcctcag atcgttacta cacgcaggat attacgcaag agcgcttcaa acttaaccca
      421 gatagcacca tcgacgtgcc cgatgcccct ggtttgggtg tgagcattga tcaggatgcc
      481 ctgaataaat tcacaattaa aaaagatctt ctcaagtgat cttagacagg aatctccata
      541 gtatcacaca gcgttcctga cgccgcactt tcccctactc tctatggatg gcgacttctt
      601 gaggggtcat acatactacg accagctctt tgggaccatg gaccccaatg gttagtgtca
      661 tttcaatatc tgcggtgcgc gaaggcccgg tgatcaacac cgccgaagtt gcctgtctga
      721 cttcttgcag atttaaaacc tgcgtgagat tttcatagat atcgtgctct tggatcaccg
      781 cgatgtgtac ctcgggcaac agcgaggcag tcaatgggcg ccctccaccc tcgcaaacca
      841 gaagagaccc tgtctctgca atcgccgcgc tagcacctgt cagcccgccg cgcacctcgg
      901 gatcaggttc atgatggatg gcaatcccgg ctgcgcgcag ctcttctaaa aggcccgtcg
      961 gcagataacc ctcctcccag gcctgaatgg agcggatatc ccgatcttgt aacagatcaa
     1021 gcacgtgctt tgaaagctcc tcttcgcgac aacggacgaa ttctcctcca actgcactga
     1081 tttcattcga aaattgcacc agccgcgcca aggcttgtgc ctgctcatca atggctggtt
     1141 gatcgctgga ctgctgacca acgtttggag gaagctcagt cgattcgcca ttcaccgaat
     1201 gccgactggc tgagtaatca ttctcacgga tctcgcgcca cctggcccga aaaggcttca
     1261 gcgcaggacg tggaaaatcc tttgaatagc cccaaccggt gatcgccggg aaacgcatcc
     1321 acatggatac aggcgaagcc aaatagccga aaaaacttgc caaacgctgc gccagggaga
     1381 aacggctagc cgaagtcgcg aaccaggtat acatgcggat accaaatgcc aaccagccgg
     1441 gtgcgtttga gatgggcgtt ttgttatcgg actcacccgg cttatcttcc atagggggtg
     1501 gttttaatgg ctgcacatct ccggcgcgca cgcgcagcaa caatttcggc aggtcgatac
     1561 ctaccgggca aacatcctca caagccccgc acaggctgga ggcgcgcgcc agattcccaa
     1621 aatcctgtcc aaaaagacct tgtgagataa ccgagccaat cggtccagaa tatgggctgc
     1681 tcttaccaga tggatttaca taggcgtgac caccgatctc gcgaaatacc gggcaggcat
     1741 tcaggcatgc accgcaacgg atgcaataaa gcgcttcaac caggggcgat tggcgcaggt
     1801 tggtgcgccc gttatccagt aggatcaggt atcgctgttg cggcccgctc ggtgcatcta
     1861 ggtgagctgg cccattgatc aaattggtgt acacgctgag cttctgccca gtggctgagc
     1921 gcggcaacag gtatagcatg agggcaagat cgtccagact gggcaccagg cgctcaatgc
     1981 ccatgagcgc aatgtgtact ttaggcaagc tggtgaccat acgcccgttg ccttcgttgg
     2041 tgacaacaca cagggtgccg ctgtccacca caccaaaatt gacccctgaa aggccaatct
     2101 gtgcatcgag aaacacctgt cggaggaccc gtcgcgctgc cagggtcagc gtggggatgt
     2161 ctacggtgta tggtattcct agtttttcgt agaaggtttg cccaacttgt tcccgccgta
     2221 agtgtacagc cggggtgatg atgtgcgccg ggtgctcacc gcgcagctgc acgatgtact
     2281 cgcccaggtc ggtttcaacc acgcgcaagc ccgccgattc caagatctgg ttcaacccga
     2341 tctcctcgga gaccatgctc ttggctttgg cgaccagttg cgccccattt tggcgggcaa
     2401 tctccaaaat agtgttcgcc gcctgcgtcc catcagcggc tcgcagcacg gtcataccat
     2461 tcatctcggc gcgctcaatg aatttcgcta acaactcatc cagatttgct atcaccaccg
     2521 cccgaacagc atgagctcgc tgcctcatct cctcccaggg tcttggcaag ctggaaaacg
     2581 cctgctggcg ggcattcaga cggcgcacag cgttggcgtg cagggctact tgcagattct
     2641 catccgccag tgctgagcga atatggtcac gaaatgctgt gtctgccaca agtattgcct
     2701 tgatggtcaa atatcataga tcacttctat ccaatatctg ggcgatatgg atagcccgcc
     2761 tcggcttccc agaccggttt aaccccccgt tgatgtgggt gatgcacccc ccgtcacagg
     2821 acactaccca atctgcgcca ctggtctcaa tattagcgat tttccgctct aacatcgcgg
     2881 cagataattc aggatgctct aaagagaaaa ctccgccaaa tccgcagcac tccgcagttt
     2941 cacgcaattc aac
//
LOCUS       DBNGSX010000032        11640 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1981, whole genome shotgun sequence.
ACCESSION   DBNGSX010000032 DBNGSX010000000
VERSION     DBNGSX010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11640)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11640)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11640
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1981"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4798
                     /locus_tag="AC16CB_01145"
     CDS             <1..4798
                     /locus_tag="AC16CB_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR3341099.1"
                     /translation="GETDQQARLTARLARAAWYMGDLPKGLEICRQGLAAIPNYKELE
                     SPGVASLIHETARAYFFNDMPEEALPLCQHALDLARRLGLVEVQAETLATLGVLPNQS
                     SEDALQALLQAVELSESAGLLAPAVRAHTNLGTHLQNIGEFISAREHLMRSREVAHQL
                     GIPDWEHRMLTNVVDICFLLGDLVYIQTVVAELNELIQCIPNPEYSLLLNQFIEARMH
                     RFKGEIDEALTTLRRCSESAKQSGRTRIEAKNNVIWGDLLIELGKIEEAEKIIAETLQ
                     IGEYVDEEDKVIAQLLMGTVRTKQGRYDEAHQLIDSIKAQIVNNPTIGTKGYLAWSEA
                     HLAGAEKNWQTAFEYFQNLDQMTSQLKLPWYRARALVDWAAVHINRGEADDLLRSKEL
                     FEEAIQIYTDLGLPRYAARVEQIGHNLPKLEFHISRLARDRYDFEDTLFSLTGNVIFA
                     NFHAARLFAQKMNQKRDLINYPERAVRPGQINAMGLIDEILHLVISIYIQQRNPRAMR
                     EALAWLYDNLGQDTVDTTLLRFTEEFPPVSVYRRQLESDEYLSGESGGVSHILLALEE
                     MLMLSITNANPACGPYLELFDDSNLEKVTRYADIMAGLREFFDRQPYFGPDNQSLVDM
                     LRSPAVAVPHSLSGQLEFIRTRWGSLLGQHLYRLLSSLDLIKEEEKIYFFGLEPATAP
                     VIEFTGQELETERFSPDMDWMPRLVLIAKNAYVWLDQLSKKYQRPIKRLDQIPDQELA
                     DLERWGITGLWLIGVWERSSASKRIKQLRGNPEAEASAYSISSYEISADLGGDDAFQE
                     LQRIAWAHNIRLASDMVPNHMGIDSRWVVEHPDWFISTDYSPFPSYTFDGPDFSEAPR
                     VGLYLEDHYFDNSDAAVVFKRLDHWTGEVKYIYHGNDGTSFPWNDTAQLNYLKPDVRE
                     AVIQTILQVARKFSVIRFDAAMTLAKRHYQRLWFPEPGSGGDIPSRAEHGMTKAEFDA
                     AMPIEFWREVVDRVAQEAPGTLLLAEAFWLMEGYFVRTLGMHRVYNSAFMNMLRDEKN
                     QEYRLVIKNTLEFDPEILKRYVNFMNNPDERTAIDQFGAGDKYFGICTMLATLPGLPM
                     FGHGQIEGFTEKYGMEYRRAYWDEQPNQYLIERHERDIFPILRRRHIFAEAKNFLLYD
                     FFTPQGNVNEDVFAYSNRSGDERALVIYNNKYASTNGWIRTSSFYLLKSGDGTDSSPD
                     SGGGASTTSRQLVQKNLGAGLELTDDPNTYTIFSDQISGLEFIRNNHDLHQNGLYIEL
                     GAYQYHVFLEFRQVQDDDWHQYSQLNTYLNGRGVPSIDEALKQILLQVVQHPFRELFN
                     PGFIHWMRDQDTELPTALDDFESRVNRLLNGVRTIAGGESELGSIASIIRADAEALLR
                     LTGLPDRMPERTSAKTMAAINYLLSGPGEELPLDEDNQFVWVILYAWIICRRLGEVVN
                     PLDPGEISRSWIEEWQLGKMMAEMLREMGNDDASIESAVTLVKLLCSHQVFSGELDLG
                     AGAERGLLYTNWLRTWLQDVELQRFLKFNRYQGFLWFNREIFEDWLWWNLATAMIDLT
                     SGIPETIEIETRKSIAARIDIVRRLLKAAESSKYQVEILLEKARGK"
     gene            complement(4879..6285)
                     /locus_tag="AC16CB_01150"
     CDS             complement(4879..6285)
                     /locus_tag="AC16CB_01150"
                     /inference="COORDINATES: protein motif:HMM:NF015052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HIR3341100.1"
                     /translation="MQSFSIHAHFYQPPREDPFTGIIPIESGAAPFINWNERIHSECY
                     RPNAELGNFERIGYNIGPTLFEWMAAYDPIASQRIVAQDRANIDRYGVGNAIAQPYHH
                     TILPLATRYDKKTQIVWGIEQFKHRFGRKPQGMWLPETAVDEESLITLVDQGVEFTIL
                     APWQAEDDNLDVTEPYRVRLPEGREITVFFYHGWLSGKVSFDPMATMNADQFVRLHLK
                     PHFRPEKIQRGEPQLLMIASDGELYGHHQSLRDHFLAHLVNGAASQESLATTFPALWL
                     REHPLRQSVKIRERTSWSCIHGVARWVGSCSCVPGNQGWKSHLRHALDRFSQQLDVLY
                     HTAVSPFVKDPWALRDDYIRALLGLQPVEDLIAQATGKRLPEEICVRIQILLESQLER
                     QKMYASCGWFFEDFERIEPRNSVAYAAKALHLTNRATEVDLAPELMANLEKVVSSRAG
                     LRGDQLLNDYLSRSVLSK"
     gene            6421..7941
                     /locus_tag="AC16CB_01155"
     CDS             6421..7941
                     /locus_tag="AC16CB_01155"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033840 - alpha-1,4-glucan
                     glucosyltransferase (NDP-glucose donor) activity [Evidence
                     IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HIR3341101.1"
                     /translation="MKYPIKVLFLAAEANPFIKVGGLADVAGSLPHTLRSLRVRSTTK
                     GASANSDEIPAVDVRLVIPLHNAIRNRNLPLKVTTEFTIEHAQGPIPVQVFETDRNGM
                     IVYLIGSELISDDEHLYHADASQDGPKYTLFSLAALELVRKLKWSPDVLHANDWHTAL
                     AVYALDILRSSERFFKKSISVLGLHNLPYLGVGAGDALAPFGLTPAYGSALPWWAQDM
                     PLPMGLLTANHIVTVSPGYAQEILTPEYGSGLDEFLQSRSQDITGILNGLEIQDWDPN
                     SDLTLAANYSLDRLESRRANKAALQNELGLPIDPDIPLLTMVTRLDPQKGVDLLPNAL
                     RQVADLAWQAVILGTGTPSLEGMMRALETSFPDRLRAVVRFDSKLSRRIYSAADAILI
                     PSRYEPCGLTQMIAMRYGCIPIAHATGGLRDTIQDYADFSHSTGFLFQEATSENLGSA
                     LRRALRVFSERELWHAMQIRGMQKDFSWESSAKQYLDLYVSLITSYGKRKLQQVNK"
     gene            7938..9200
                     /locus_tag="AC16CB_01160"
     CDS             7938..9200
                     /locus_tag="AC16CB_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase family
                     protein"
                     /protein_id="HIR3341102.1"
                     /translation="MKTRAVILAGGEGSRLGILTAKRTKPAVPFAGKYRIIDFTLSNC
                     VNSGLFDIMILAQYRPHSLIDHIGAGNPWDLNRDFTGGVRIFTPYKARGSSDWYLGTA
                     DAVQQNFRFIKSGDPELILILSGDHIYEMNYDALIAFHLDHDADLTMATIRVPISDAS
                     RFGIVGVDNRFRVTTFVEKPAQPPSNLVNMGVYVFNFKALDQALLDDSEKTGSSHDFG
                     KDILPRMIQNQARVFAFPFSGYWVDVGTVESYWQAHMDLLYDNPPFDLNDRSWIIHTR
                     TEERPPAWISGGAVVIDSMITDGCVIAPGARIERTVLAPGVRIHAGAIVRESVLLTDT
                     LIEEGAVVERAVIDKRAVIGESARVGRSQPDEIPKIAMIGKNSYVPPGYIIEPGAVIA
                     TDVAQEDYPSKIIREGDYIQTKRPPYEV"
     gene            9190..10683
                     /gene="malQ"
                     /locus_tag="AC16CB_01165"
     CDS             9190..10683
                     /gene="malQ"
                     /locus_tag="AC16CB_01165"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HIR3341103.1"
                     /translation="MKFERASGILLHPTSLPGPYGIGDIGAGAHLWLEFLAKTGCSLW
                     QILPLGPTGYGDSPYQCFSAFAGNPYLISPEALLNDGLLFPDDLADIPAFSDERVDFG
                     LLIPWKLEILDQAYQRFEEHASDELRSAQAAFQQTHASWLDDYALFMALKDENGGASW
                     VTWAEPVRDRHPQALQQAKQRLAVSIKRHVFRQFIFFRQWGALREKAVDLGIRIIGDI
                     PIFVAHDSADVWARPELFFLESDGNPSAVAGVPPDYFSPTGQLWGNPLYHWSVHRDNG
                     YSWWLDRFQAVLSLVDIVRLDHFRGFAGYWRVHGKAKTAEKGRWMRGPGKDFLQAVRV
                     ALGDLPIIAEDLGVITPDVVRLRDGFKLPGMKILQFAFEGDPLDPFLPHNYPENCVVY
                     TGTHDNDTARGWYERINEDARHFCRRYLNSDGSRIAWDMIRACWASVAIFALAPLQDF
                     LELGNEARMNYPGNPSGNWTWRMREDIQLDELSEQIQELNYLYSRLK"
     gene            complement(10694..11278)
                     /locus_tag="AC16CB_01170"
     CDS             complement(10694..11278)
                     /locus_tag="AC16CB_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341104.1"
                     /translation="MPRQFRNIILLMAFLGLVQGSAACDSTQATPQPIPGATSTPTQI
                     LSPQIEIVTEEPQIDTPVPIMEEESDLDPYVKVEVSEINLNLGETVSVTGWPVDINLP
                     NYYLILRDEGIQDNPFTAMVTHDNQVTPGEGRSKVVEWVSAEGKVDQVIFVLRAIGEG
                     VTAMDIEAIGELQLQDGSTLSVAGSGSVLIIVGD"
     gene            complement(11282..>11640)
                     /locus_tag="AC16CB_01175"
     CDS             complement(11282..>11640)
                     /locus_tag="AC16CB_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341105.1"
                     /translation="AHIHRAHHPSPESELRLASVAEFVAAVSIGALIVGIVLLIVFAR
                     HYLLIGLAAMIGVLIFIEAGFRRQLHRLINSLTVGLAIVSAMVLLFEFFWQIVVIGVL
                     AAGLYIMWENLREITG"
BASE COUNT         2967 a         2952 c         2869 g         2852 t
ORIGIN      
        1 tggcgagaca gatcaacaag cgcgcttgac tgcccgatta gcacgcgccg cttggtatat
       61 gggagacctc cccaaagggt tggaaatttg ccgccagggt ttggctgcca ttccaaacta
      121 caaggaactt gagtctccag gagttgcttc cctaatccac gaaacggccc gcgcctattt
      181 tttcaatgac atgccagaag aagccctacc tttatgccag cacgcactag atttggccag
      241 gcgtcttggc cttgtggaag tgcaagctga aacgcttgct acacttggag tactgcctaa
      301 ccaatctagt gaagatgcct tgcaagcgtt attacaagcc gtagagcttt ccgaatccgc
      361 cgggttgctt gcccccgccg tgcgtgcaca caccaacctt ggcacacatt tgcaaaatat
      421 aggggaattt atcagcgcac gtgagcatct gatgcgcagt cgcgaagtag ctcatcaatt
      481 aggtattccc gattgggagc atagaatgct caccaatgtt gtagatattt gttttctctt
      541 aggagatctc gtctatatcc agactgtggt tgcggagcta aacgaactca tccaatgtat
      601 tccaaatcca gagtactcgt tattgttaaa tcaatttatc gaagctcgta tgcaccgctt
      661 caaaggagaa atagacgaag cacttacaac cttgaggaga tgcagcgaaa gtgcaaagca
      721 atccggtcgt acacgtatag aagccaaaaa taatgtcatc tggggagatc ttttgatcga
      781 attaggaaaa attgaagaag cggaaaaaat tatcgctgaa acgctacaga ttggagaata
      841 tgtagacgaa gaggacaagg tgatcgcaca attgctaatg ggcaccgtac gcacgaagca
      901 gggcaggtat gacgaggccc atcaattgat cgactcgata aaagctcaaa tagtgaataa
      961 tcccaccatt ggtactaagg gatatttagc ttggtcggag gcccatctcg ctggcgcgga
     1021 aaaaaactgg cagacagctt ttgaatattt tcaaaacctc gaccaaatga catctcaact
     1081 aaaacttcct tggtatcgag cccgcgccct ggtagattgg gctgcggttc acataaaccg
     1141 gggtgaagca gacgatcttc tcaggtccaa agagttattt gaggaagcga tccaaattta
     1201 cactgatctt ggtctgcctc gctacgccgc ccgtgttgaa cagataggtc acaacctccc
     1261 gaaattggag tttcacatct cgcgcctggc acgcgaccgc tatgacttcg aagacacact
     1321 tttctcacta accggcaatg tgatcttcgc caatttccat gcagctcgcc tttttgcaca
     1381 aaagatgaac caaaagcgcg acctgatcaa ttacccagaa cgagcagtgc gacccgggca
     1441 gattaacgcc atgggattga ttgatgagat cctccattta gtcatctcca tatatataca
     1501 gcagcgcaac ccgcgcgcaa tgagggaagc tctggcgtgg ctatacgaca acctgggtca
     1561 ggatacggtg gataccaccc tgctacgctt taccgaggaa ttccctcccg tgtcagttta
     1621 ccgccgccaa ttggagtcgg atgagtattt gagcggcgaa agcgggggtg tgtcgcacat
     1681 cttgctggcg cttgaagaga tgctcatgct ctcgattacc aacgctaatc cagcctgtgg
     1741 cccttatctg gagttatttg acgattccaa cctcgagaaa gttactcgtt acgctgatat
     1801 catggctggc ttgagagagt ttttcgaccg ccagccctat ttcggcccag ataaccagtc
     1861 tcttgtggat atgttgcgta gcccggcggt cgcggtaccc cattcgcttt ccggacaatt
     1921 ggaatttatc cgcacacgct ggggtagcct gctaggacaa catctgtacc ggctcttgag
     1981 cagtcttgac ctgatcaaag aagaggaaaa aatctacttc ttcggactcg aaccagcaac
     2041 tgctccagtg attgaattta ccggtcagga actggaaaca gagcgtttta gcccggatat
     2101 ggactggatg ccgaggctgg ttctcattgc gaaaaatgca tacgtctggc ttgatcagct
     2161 atccaaaaag taccagcgtc ccatcaagag actggaccag atacccgatc aagagctagc
     2221 cgatctggag aggtggggta tcactggttt gtggctgatt ggcgtttggg agcgtagctc
     2281 cgcttcaaag cgcatcaaac aactgcgcgg caatcccgaa gcggaggctt cggcttattc
     2341 gatctcgtca tacgagattt cagccgattt ggggggtgat gacgcttttc aagagctgca
     2401 acggatcgca tgggcgcata acatccgttt ggcgtccgat atggtaccaa accacatggg
     2461 cattgattcc cgctgggtgg tcgaacatcc tgactggttt atctcaacgg attatagccc
     2521 cttcccttcg tacaccttcg atggtcccga cttttcagaa gctcccaggg tcggtttgta
     2581 ccttgaagac cactattttg ataacagcga tgcagcggtt gtctttaagc gattggatca
     2641 ctggacaggt gaggtgaaat atatttatca cgggaatgat ggcacttcct tcccctggaa
     2701 cgatactgcc cagttaaact atctcaagcc cgatgttcgc gaagcagtca tccagaccat
     2761 cctccaggta gcgcgcaagt tctccgtgat ccgtttcgat gcagccatga cactggcaaa
     2821 gcgacactac cagcgattat ggtttcccga gcctggaagc ggaggcgata tcccatctcg
     2881 ggctgagcat ggcatgacca aagccgagtt cgacgcagcc atgcccattg agttttggcg
     2941 cgaagtggtg gaccgcgtcg ctcaggaagc acccggtacg cttttgctgg cagaggcttt
     3001 ctggttgatg gaaggttatt tcgtgcgaac tctcggcatg caccgcgtat acaacagcgc
     3061 attcatgaac atgctgcgag atgagaaaaa ccaggaatac cgcttggtga tcaaaaatac
     3121 tttagaattt gatcctgaga ttctcaaacg ttacgtgaac tttatgaaca acccggacga
     3181 gcgcaccgcg attgatcaat ttggcgcagg agacaaatat ttcggcatct gcacgatgtt
     3241 agctacgctg ccgggattgc ccatgttcgg tcatggccaa atcgaaggct tcacagagaa
     3301 atatggcatg gaatatcgcc gcgcatattg ggatgagcaa ccaaatcaat atctgatcga
     3361 gcgccatgaa cgggacatct tcccaattct gcgtcgacgc catatctttg ctgaggcgaa
     3421 aaacttcttg ctctacgact tttttacccc gcaagggaat gtcaacgaag atgttttcgc
     3481 ttattcgaat agatctggcg atgagcgggc gcttgtgatc tacaacaaca aatatgcttc
     3541 aacaaacgga tggatacgca cttcatcttt ctacctgctg aagtctggcg atggtaccga
     3601 tagctcacct gattcaggtg gaggcgcttc cacaactagc aggcagctag ttcaaaaaaa
     3661 cctgggagct ggcttagaac taacggatga cccgaacacc tatacaattt tctccgatca
     3721 aatctccggt ctagagttca tccgaaacaa ccacgatctc caccaaaacg gactctatat
     3781 cgagctgggc gcttaccaat atcacgtttt tctggaattt cggcaggtgc aggatgatga
     3841 ttggcatcag tatagccagc tcaacacata tttaaacggg cgtggtgtgc catctattga
     3901 tgaggcgctt aagcagattt tgctacaagt tgtgcaacat ccatttagag aattattcaa
     3961 tcccggtttc attcattgga tgagagacca agacactgaa ctgccaaccg ccctggatga
     4021 ttttgaatct cgcgtaaatc ggttattgaa tggcgtccgc acgatagcag gaggagaatc
     4081 tgaattgggt tctatcgcct cgattattcg cgccgatgcg gaggcgctgc ttcgtctcac
     4141 cggtcttcca gatcgcatgc ccgaaagaac gagcgcaaaa acgatggccg caataaacta
     4201 tttgctatct ggaccagggg aagaattgcc gctggacgaa gacaaccaat ttgtatgggt
     4261 tatattgtac gcctggatca tttgccgaag acttggagag gtcgtaaatc ccttagatcc
     4321 gggagagatc agccgttctt ggattgaaga gtggcaactc ggaaagatga tggctgaaat
     4381 gctacgagag atggggaatg acgatgcatc tatcgagagc gctgtcaccc tagttaaatt
     4441 gctttgcagc catcaagtgt tctctggtga acttgatctt ggagcaggag ccgaaagagg
     4501 attgctatac actaattggc ttcgaacctg gctccaagat gttgagctac agcgctttct
     4561 taaattcaac cgctaccagg gtttcctatg gttcaatcga gagatatttg aagattggtt
     4621 atggtggaat cttgccactg ccatgatcga tctcaccagt ggaattcccg agaccatcga
     4681 gattgaaacc aggaaaagca ttgcggctcg gattgatatt gtcagacgcc tacttaaagc
     4741 cgcagagagt agcaaatatc aggttgagat actactcgaa aaagctcgag ggaaatagca
     4801 tttctttatt ttctttttta cttcatggta aatgctatga aacggcatct attacacata
     4861 ctgatcagtt tattgtgatc atttagatag tacacttctg cttaaataat catttaagag
     4921 ctggtcgccc cgcaagccgg cgcgagagct aaccactttt tcaagattgg ccattaactc
     4981 aggcgccaaa tcgacctcag tagccctgtt tgtcaagtgc aaagctttgg ctgcataagc
     5041 cacgctattg cgcggctcta ttcgctcaaa atcctcaaaa aaccatccac aagaagcata
     5101 catcttctgg cgctcgagct gagattctaa aagaatctgg atgcgcacgc aaatctcctc
     5161 tggcaatcgc ttcccagttg cttgagcaat taaatcttca actggctgca aaccgaggag
     5221 ggcccggatg taatcgtcgc gcaaagccca gggatctttc acgaaaggtg acacagctgt
     5281 gtgataaagc acgtccaatt gttgtgaaaa ccgatctaaa gcatggcgca gatgagattt
     5341 ccagccctgg ttacctggca cacaggaaca actaccaacc cagcgtgcca caccgtgaat
     5401 gcagctccag gatgtgcgct cacgaatctt gacagattgc cgcagtggat gctcccttaa
     5461 ccataaagcc gggaaggtgg tagccagact ttcctggctc gccgcaccgt taacaaggtg
     5521 agctagaaag tggtcacgca gcgattgatg atgaccatat agctctccat ctgaggcaat
     5581 catcaatagc tggggctctc cgcgctgtat tttttctggg cggaaatggg gtttcaggtg
     5641 caggcggaca aattgatcgg cattcatcgt tgccatcggg tcgaagctaa ctttgccgct
     5701 aagccatcca tggtaaaaaa agaccgtgat ctctcgccct tctggcaagc gaacacggta
     5761 cggttcggtc acatcgagat tatcatcctc agcttgccag ggagccagga tcgtgaactc
     5821 aacaccttgg tcaacgaggg tgatcagaga ttcttcatct accgctgtct ccggcagcca
     5881 catgccttga ggttttcgcc cgaaacggtg cttgaactgc tcgatacccc aaacgatctg
     5941 cgtcttttta tcgtaacggg tggctaaggg gaggattgtg tgatggtaag gctgagcgat
     6001 ggcatttccc acaccatatc tgtcgatatt ggccctgtct tgtgccacga tgcgctggga
     6061 ggcgattgga tcataggctg ccatccactc aaaaagggtt ggtccaatat tgtagccaat
     6121 tcgttcaaag ttacctagct cggcgtttgg acggtagcat tcagaatgga ttcgttcgtt
     6181 ccagttaatg aaaggtgccg ccccagattc aattggaatt atccctgtaa aagggtcctc
     6241 tcggggcggc tgatagaagt gggcgtgaat actgaatgac tgcaatggat ttctcagatt
     6301 atgtttatca tatatattat attcgattat gtgatttaaa ccacataatc gtgtaaaatt
     6361 gattctaccc atcctcttgt gctcagaaac aaggacagga atctcgcagc caatactctc
     6421 atgaagtatc caatcaaagt tctctttctc gcggctgagg ccaacccatt cattaaggtc
     6481 ggtgggctgg cagatgtagc cggttccttg ccacatacgc ttcgttctct gagagtacga
     6541 agtaccacca agggtgcttc ggcaaattca gatgaaattc cagctgtcga tgtgcggctg
     6601 gtaattccgc tacacaatgc catccgcaac cgaaatttac cgctgaaagt taccaccgaa
     6661 ttcactatcg agcatgctca agggccgatc ccagtgcagg tttttgaaac cgatcggaat
     6721 ggcatgatcg tctacctgat tggcagtgag ttgatcagtg acgacgagca tctctaccac
     6781 gccgatgcaa gccaggatgg gcccaaatac acgttgttct cactcgccgc tctcgaacta
     6841 gtccgcaaat tgaaatggtc gcccgatgta ttgcacgcca acgattggca cacagccttg
     6901 gcagtttacg ccctagatat tttgcggagc tcagagcgct tttttaagaa aagcatctcc
     6961 gtactaggtc tccacaactt gccatacctg ggtgtgggag caggagatgc actggcaccc
     7021 tttggattaa cacccgctta cggctccgct ctcccctggt gggcgcagga tatgccccta
     7081 ccgatgggct tgcttacagc caaccatatc gtgactgtct cacctggtta tgcccaggaa
     7141 atcctcaccc ccgagtatgg ttcgggacta gacgaattcc tccaatcgcg ctcccaggat
     7201 atcactggca tcttgaatgg tcttgaaata caggattggg atccaaactc agaccttacc
     7261 ctagcagcca attattctct ggacaggcta gaatctcgtc gcgccaacaa agccgccctg
     7321 caaaatgagc tgggcttgcc catcgaccct gatatccctc tgctcacgat ggtcacccga
     7381 ctggatccgc aaaaaggcgt cgacctgctg cccaatgcgc tgcgccaggt agctgatctc
     7441 gcctggcaag cagtgatcct ggggacaggc accccctccc tcgaagggat gatgagagcc
     7501 ttagagacca gttttccaga tcgattgcga gctgttgtcc gcttcgactc gaagctaagc
     7561 cgacgtattt actccgcggc tgatgcgata ttaatccctt ctcgatacga gccttgtggg
     7621 ctaacccaga tgatcgcaat gcggtacggt tgcatcccga ttgctcacgc cacgggtggg
     7681 ctgagggaca caatacagga ctatgccgat tttagccaca gcactggatt tcttttccag
     7741 gaagctactt ccgagaacct cggctccgcc ctccgccgag ctttgcgtgt tttttcagaa
     7801 cgcgaactct ggcacgcgat gcagatacgc ggtatgcaaa aggatttttc ttgggaaagt
     7861 tcagccaaac aatatcttga tctctatgta tctctaataa cgtcttatgg taagagaaag
     7921 ttacaacagg tgaataaatg aaaacacgtg cagtcattct ggctggtgga gagggttcac
     7981 gccttggaat actcaccgcc aagcgcacaa aaccggctgt cccctttgca ggtaaatacc
     8041 ggattattga tttcacactc tcaaactgcg tcaattctgg tttgttcgac atcatgatct
     8101 tggctcaata ccggccccat tcacttatcg accatatcgg ggcaggaaat ccgtgggacc
     8161 tcaatcgcga ttttacaggg ggtgtgcgta tatttacccc ttacaaggcg agaggctcat
     8221 cagactggta tctaggcaca gccgacgctg tgcagcaaaa ctttcgcttt atcaagagcg
     8281 gcgacccaga attgatcttg atcctatccg gtgaccatat ttacgagatg aattatgacg
     8341 cgctgatagc attccacttg gatcatgacg cggatctaac gatggctacc atccgcgtgc
     8401 caatatcaga cgcttcacgt tttggtatcg ttggcgtaga taatcgattt cgagttacaa
     8461 cattcgttga aaagccagcc caaccccctt caaacctggt taatatgggt gtatacgtat
     8521 ttaactttaa agcccttgac caggctcttc tggacgatag cgagaaaacc ggaagctcac
     8581 acgactttgg aaaggatatt ctcccgcgca tgatccaaaa ccaggctcgc gtatttgcat
     8641 ttccctttag cggatactgg gtagatgtgg ggacggttga gtcctactgg caagcacata
     8701 tggacctgct atacgataac ccacccttcg acctgaacga tcgttcatgg atcatccata
     8761 cccgtacaga agaacgccct ccagcatgga tatcaggtgg tgcagtcgtg attgacagca
     8821 tgatcaccga tggctgtgtg attgcgcctg gagcgcgtat agaacgcact gtgctagctc
     8881 caggcgtgcg gattcatgcg ggtgcaatcg tccgagaatc tgtcttgctg acggatacac
     8941 tcatcgaaga aggggctgtg gtagaacgcg ctgtcatcga taaacgcgcg gttatcggag
     9001 aatctgcccg cgttggacgc agccagcctg atgagatacc aaaaattgcc atgatcggca
     9061 agaatagcta tgtgccacca ggatacatca tcgaaccagg tgcagtgatc gcgactgatg
     9121 tcgcccaaga ggattatcca tcgaaaatca tccgcgaggg tgattatata caaaccaaga
     9181 ggccgcctta tgaagtttga acgtgcttcc ggcatccttc tccaccctac cagcttgcct
     9241 ggaccttatg gcatcggcga tatcggggcg ggcgctcacc tgtggttaga atttctggca
     9301 aaaaccgggt gctctttgtg gcaaatctta cccttggggc ccaccggtta tggcgattcg
     9361 ccttaccagt gtttctccgc ctttgctggc aatccatact tgataagccc tgaagctttg
     9421 ctaaacgatg ggttattgtt tcccgatgac ttggctgata ttccagcatt ttctgacgag
     9481 cgggtcgact ttggcttact tataccctgg aagcttgaaa tcttggatca agcctatcag
     9541 cgctttgaag aacatgcttc agacgaactg cgtagtgcac aggcagcttt tcaacaaacc
     9601 cacgcatcct ggctggatga ttacgcctta tttatggcac ttaaagatga aaatggcggg
     9661 gcttcatggg tcacttgggc tgagcctgtg cgcgaccgcc atccgcaagc tctacaacag
     9721 gcaaagcaaa gactggcggt gtcaatcaaa cgccatgtct ttcgacagtt cattttcttc
     9781 aggcaatggg gcgcgctgcg cgagaaggct gttgatttgg gtatccgcat tatcggcgat
     9841 atcccgatct tcgttgccca cgacagcgcg gatgtttggg cacgccccga attgtttttc
     9901 cttgaatcag acggcaatcc cagcgcagtt gcgggagtac caccggatta tttttcgccc
     9961 accggacaat tatggggtaa tccattgtac cactggagtg tgcaccggga taacggttac
    10021 agctggtggt tggatcgctt ccaggccgtt ctaagcctcg ttgatatcgt tcggttagac
    10081 catttccgag gctttgcagg gtattggagg gtgcatggaa aagcaaagac agcagagaag
    10141 ggtcgttgga tgcgcggtcc gggtaaggat ttcctccaag ctgtgcgagt cgcgctagga
    10201 gatctaccca tcatagccga agacctaggc gtgattacgc ccgatgtcgt caggttacgt
    10261 gacggtttta agctcccggg aatgaagata ctgcaattcg cctttgaggg cgatccacta
    10321 gaccccttct tgcctcacaa ttacccagaa aactgcgtgg tctatactgg cacccacgat
    10381 aatgacaccg cacgcggctg gtatgaacgc atcaacgagg atgccaggca tttttgccgg
    10441 cgctacttga acagtgatgg aagtcggatt gcctgggata tgattcgtgc ctgttgggcc
    10501 tctgtggcaa tcttcgccct ggcacccttg caagattttc tagaattggg caatgaagcg
    10561 cgcatgaact accccggaaa tcctagcggc aattggacat ggcggatgcg cgaagatatc
    10621 cagctcgacg agttaagcga acagatacag gaattgaatt acttgtactc tcgcttgaaa
    10681 tagggataaa taatcagtcc ccaacaatga ttaatactga tcccgaaccc gccacactca
    10741 gggttgagcc atcttgaagt tgcaattcac cgatagcctc aatgtccatg gcagttaccc
    10801 cctcgccgat ggcgcgcagg acgaatatca cctgatccac cttaccctcg gcagagaccc
    10861 attccaccac cttacttctc ccttcgccag gtgtaacctg gttatcgtgc gtcaccatgg
    10921 cggtgaaggg attatcttga atgccttcgt cgcgcaaaat caaataataa tttggtaaat
    10981 tgatatcaac tggccagcca gtgaccgata ctgtttcccc aaggttcaga ttaatttcac
    11041 tgacctccac cttcacataa gggtctaaat cgctctcctc ctccattatc gggacggggg
    11101 tatcgatttg tggctcctca gttacgatct caatttgggg agacagtatc tgcgtggggg
    11161 tactggttgc cccaggtatg ggctggggag tggcctgtgt agaatcacac gccgcagaac
    11221 cctggactaa cccaagaaat gccattaaaa gtatgatgtt cctgaactgt cgaggcataa
    11281 attatcccgt aatctcacgc agattttccc acataatata caagcccgcc gccaatacgc
    11341 caatcacaac aatctgccaa aagaactcaa atagcaagac cattgccgaa acgatcgcta
    11401 gtcccacagt caggctgtta atcaggcggt gtaattgacg gcggaagcct gcttcgataa
    11461 agataagcac gccaatcatc gcggccagtc caatcaataa ataatggcgg gcgaacacaa
    11521 tcaaaagcac aataccgaca atcaatgcac cgatgctcac tgcagcgaca aactctgcca
    11581 cactggccag tcgcagctca ctttcaggcg aagggtgatg agcacggtga atatgagccc
//
LOCUS       DBNGSX010000033         3363 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_902376, whole genome shotgun sequence.
ACCESSION   DBNGSX010000033 DBNGSX010000000
VERSION     DBNGSX010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_902376"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1658)
                     /locus_tag="AC16CB_01180"
     CDS             complement(93..1658)
                     /locus_tag="AC16CB_01180"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3341106.1"
                     /translation="MLPDVRVRQRDYLLEISRAITQELNLDKLLTRILDISAEMLAGQ
                     AGLIALRSERGGWMVAASHGIPPAFLRYLDPLLAAVPDHEDPARFELPEINRLLQNLT
                     RMASLGLLTGVGLPMIVRKQVIGVVFIFRNYKGVFSANDRALLESFADQAAIAVQNAQ
                     LYTQVSREKQRMDALLDSAADGILIIGADHTIERCNPAFARMIGRSMDSIRKRSHEEV
                     IRWKRKDGLTLEQAEAGGWPLTPNATLYIEGDLQREDGLPLPVGITYAPLVNPEGNLV
                     NIIATIRDITRFREAEELKSTFISVISHELKTPVALIKGYVSTLRRDDASWDRKVVQD
                     SLEVIEEEADRLTELIENLLDASRLQAGGLVLSTTDVALNKIAERIAERFRTQSSLHT
                     IGVDFPPDFPVVLADEERISQVISNLISNAIKYSPEGGEVRISGQVLPDHVIVCVSDQ
                     GPGIAPDDIPHVFDRFYRSSIAVRTTKGAGLGLYLARAVIEVHGGRIWVDPKPGDGTR
                     VCFSIPRTYPVNY"
     gene            complement(1648..2985)
                     /locus_tag="AC16CB_01185"
     CDS             complement(1648..2985)
                     /locus_tag="AC16CB_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HIR3341107.1"
                     /translation="MQIMNLPGPKARAILKRDQAVISPSYPRGQPFVMDHGKGCHVWD
                     VDGNRFLDFAAGVAVVATGHSHPKVVQAIQEQAERFIHISSDFYHPLWVELGEKLNEI
                     APFLDNAVSFMTNSGTESVEAAIKLARHTTGRSQFIGFLGAFHGRTMGSVSFTASKSG
                     YHRGFYPLLNGVVHVPFPDPYRPVIQSKPGEDYGETIVRYIEEEIFGHLLPTNDVAGV
                     LVEPIQGEGGYVVPSPGFFPALRKLCDRHNMLLIVDEVQSGMGRTGKWWAIEHANVEP
                     DIVCAAKGIASGMPLGAMIARESLMTWPKGSHGNTYGGNPLACAAALATIQLLEDEYL
                     HNAGEMGEYMMDILQELKVRHPSIGDVRGKGLMVGIEFVLDHQTKVPAEKLRDRVVDL
                     AFSMGLLMLGCGKSVVRISPPLSISRSEVDEAMEIFEQAVTLAEREPEILHAA"
BASE COUNT          908 a          889 c          778 g          788 t
ORIGIN      
        1 aaccccccct cctttcctct tcaactgcgt aagtcctata actaataagt aacgcaattg
       61 gcatgagatt atccatcaat tgcgtgcgtg ctttaataat tcacagggta cgtccggggt
      121 attgagaaac acacccgtgt tccatcacct ggtttggggt caacccatat acgcccacca
      181 tggacttcga taacggcacg agccaagtat aagcccaatc ccgcgccctt ggtcgtccga
      241 acggcgattg aagaccggta aaagcgatca aacacgtggg gtatatcgtc gggggcgatg
      301 cctggaccct gatcgctgac acatacaatc acatgatctg ggagaacttg accagaaata
      361 cgcacctctc caccctcggg tgaatatttg atggcatttg agattaagtt cgagatcacc
      421 tgagaaatgc gttcttcatc cgctaggaca actgggaaat ccggagggaa atccacgccg
      481 atagtatgca gactgctctg ggtgcgaaat cgttcggcga tacgctcggc gatcttattc
      541 aaagctacat ccgttgtgct gaggaccaat ccaccagctt gcaatcggga agcgtcaagc
      601 aaattttcaa tcagttcggt taaccggtca gcttcttcct caattacctc caagctatcc
      661 tggaccactt tgcgatccca ggaggcgtca tcgcgacgca acgtgctgac ataaccttta
      721 atgagagcta caggtgtttt caactcgtga ctgatcactg atatgaaagt gcttttaagt
      781 tcttcggcct cacgaaaccg agtgatatct cggatcgtag cgataatatt aaccagatta
      841 ccttcggggt ttaccagtgg cgcataagtg atccccactg gcaagggcaa cccatcttcg
      901 cgctgcaggt cgccctctat gtacaaggta gcgtttgggg ttagaggcca ccctcccgct
      961 tcagcctgct ccagggttaa tccatctttt cgcttccaac ggattacttc ttcatgcgag
     1021 cgcttgcgga tgctatccat cgagcgacca atcattcgag cgaaggctgg gttacaacgt
     1081 tcgatggtat ggtccgctcc aatgattaag atcccatctg cagcggaatc tagtagggcg
     1141 tccatgcgtt gtttttcccg gctaacctga gtgtaaagct gggcattctg tactgcgata
     1201 gcagcttgat ctgcaaaact ctctaaaaga gcacgatcgt tggctgagaa tacaccctta
     1261 taattgcgaa aaatgaatac taccccaatg acctgcttgc ggacaatcat cgggagaccg
     1321 accccggtca ggagtcctaa gctagccatc cgggttaagt tctgcaaaag acgattgatc
     1381 tccgggagct caaaacgagc cgggtcttca tgatcaggaa ctgccgcaag aagcgggtcg
     1441 agatagcgta aaaaagcggg agggataccg tgggaggcgg caaccatcca tcccccccgc
     1501 tcagaacgta atgcaattaa gcctgcctgg cctgccagca tctctgcaga tatatccaga
     1561 atgcgcgtca atagcttatc taagttgagc tcttgtgtaa ttgcgcgcga tatctcaagc
     1621 agataatctc gctggcgtac acgaacgtca ggcagcatgc aaaatctccg gctccctctc
     1681 ggctaaggtt actgcctgtt caaaaatttc catggcttca tccacctcac tgcgtgagat
     1741 agagagcggt ggcgaaatgc gaacaacact tttcccacaa ccaagcatga gcagccccat
     1801 gctaaaagcc aaatcgacca ctctgtcgcg caacttttca gccggcactt tcgtctggtg
     1861 atccaggaca aattcaatgc cgaccatcaa acccttgcca cgcacatcgc caatacttgg
     1921 gtggcgaact tttaactctt gcagaatatc catcatatat tcgcccatct ccccagcatt
     1981 gtgcaaatat tcgtcttcca ataactggat ggtcgccagg gctgcggcac aggctaatgg
     2041 attgccgcca taagtattgc cgtgcgagcc tttgggccag gtcatgagcg actcacgggc
     2101 tatcatagcc cctaggggca tacccgaagc aatgcccttg gcagcgcaga caatatccgg
     2161 ttcaacattg gcatgctcga tagcccacca ctttccagtg cgccccatac ctgattgaac
     2221 ctcatctacg atcaaaagca tgttgtggcg atcgcacaac ttgcgcaatg cagggaagaa
     2281 acccggtgaa ggtacaacat agccgccctc gccctggata ggctccacca atacgcctgc
     2341 cacatcgttg gtgggtaaaa ggtgaccaaa tatttcttct tcaatgtagc gcacaatagt
     2401 ctcaccgtaa tcctcacccg gtttagattg aatcacaggc cggtatggat cagggaacgg
     2461 cacatgaact accccgttga gcagcgggta aaagccgcga tgatatccag acttgctggc
     2521 agtgaaggat accgacccca tggttcgccc gtgaaaggca cccaaaaagc caatgaactg
     2581 cgagcgacct gtcgtatggc gggctagctt aatcgccgct tcaacgctct ctgtacctga
     2641 attggtcatg aaagagacag cattatccaa gaagggagca atttcgttta acttttcacc
     2701 cagctctacc cagagaggat gataaaaatc ggaagatata tgaatgaatc tttctgcttg
     2761 ttcttgaatg gcttggacaa ctttgggatg gctgtgtccg gtagctacaa ctgccacgcc
     2821 tgcggcaaaa tcaagaaaac gattgccatc aacatcccaa acatgacaac ctttgccatg
     2881 atccatgaca aaaggttggc cccttggata tgagggggag atgactgcct gatcgcgctt
     2941 aaggattgcc cgtgctttcg gaccgggcaa attcatgatt tgcatacttt tatctcctga
     3001 ataattaaca aatctacgtc cgcttgggac ggaatagcca aagaattata tcattggtca
     3061 taataagcga cagataaagg ctacaagcct gatcaaatac tcaaagggaa cagccaatca
     3121 tccatctcta cataatgatt ttcaaccgaa attagaagct cttcgataca aaaacatcct
     3181 gcaggacgct cttaaacaag ctgtatttag attagtaagc ggctatcaca atgtatgtga
     3241 ccgttccaaa attggtaaaa gatggattgg taaacagcat tgacggcaaa accggcgaat
     3301 aatcttcaca gctatgccat caaatcagct aaacacaggc cacgggcaag gttgttccca
     3361 gca
//
LOCUS       DBNGSX010000034         7232 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_279170, whole genome shotgun sequence.
ACCESSION   DBNGSX010000034 DBNGSX010000000
VERSION     DBNGSX010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7232)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7232)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7232
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_279170"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1490)
                     /locus_tag="AC16CB_01190"
     CDS             complement(<1..1490)
                     /locus_tag="AC16CB_01190"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HIR3341108.1"
                     /translation="MSNRFLVGVDIGTYSSKGVLVKTDGTVVASHTVPHGMDMPKPGY
                     FEHDADQVWWHDFVVITKNLLSSSGIDPKQILGIGTSAIGSCVLPIDENGKPLRPGIL
                     YGIDTRASTEIEYLERELGKHEIFRTSGSHLSSQASGPKVLWIRNHEPEIYQKTRWFL
                     TSEAYLVHKLTGKASIDIYTAGGYAPLYDVHSREWISEAAELITPLERLPKAYWSYEI
                     VGEVTAEAAQETGLAEGTPVVAGTTDAAAEAVSAGVAEFGDMMLMFGSSIFFIMKTAE
                     LIKTQSFWSSNFLEEGSFAFLGGMSTSGSLTTWFRDQLAHYEIEKEKEGGENAYAVLA
                     SLASASPIGSNGLIALPYFEGERTPLHDPKAKGVWFGLSLKHTKGDIYRAILEGVAFG
                     IRHNVEVMGEEGVQPARILAVGGGTKNPLWLQIVADVCNIDLVVPEQQIGASYGDAFL
                     AATGVGLFKDLSEIKQWVRIKETIHPNLESRKLYEINYQIFRDLYA"
     gene            complement(1514..2581)
                     /locus_tag="AC16CB_01195"
     CDS             complement(1514..2581)
                     /locus_tag="AC16CB_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR3341109.1"
                     /translation="MDKRWAQLGDLLVNYSMAVKPGEKVMIAFVEVETYPLVHAIYRS
                     CLQAGALPQVQFLSEELNRLVLVHGTPEQIGWVPEIEAYGMEWADVYFGLRGAHNLDV
                     FWDIPAEKLSLLRQAMGKISTLRWQKTRWCLLRVPNAALAHQAGVDEETITDMFFNAC
                     FLDWPVVSQEWRRWADELTKGDQIRIVGKGTELSFSTEGRTWDVAAGHSNMPDGEIAT
                     APVESTIDGVIYFDFPGVLGGRLVHDIRLRWEQGKLVEATSSTNQDFLRSVLNTDAGA
                     SLIGEFAIGTNPEVNYFCKDILIDEKIGGTVHIALGRAYPKVGGTNQSAIHWDIIKDM
                     RQEGEIYLDDELVFRNGKVLL"
     gene            complement(2613..3650)
                     /locus_tag="AC16CB_01200"
     CDS             complement(2613..3650)
                     /locus_tag="AC16CB_01200"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3341110.1"
                     /translation="MDTKSNPSTLMKSLSTLIKKIQMGSQTALLLGVFVVLFAFFAIT
                     EDRFLTVRSVTSMGFQLPELGVLSLAMMLSILTGGINLSVVATSNLAAVLAGFFMVKF
                     IPPDASPQQIMLYIAIALLIALVVGWLSGVINGFLIAHVGVPPILATLATLTFYTGIS
                     TGLTGGATVTGFPEQLSGIGNETFFGIPIPFLIFVLLTIYTYFLLNRTTFGFKARMLG
                     SNPIASNFSGIDNKSIIMNTYIVSGVFSAITGILIMSRTMSAAYEYGSTTYVLLTILI
                     AVLAGILPGFGHVVDIFIAVLILQILSTGFHMVLVGIRGSSFFKDFSWGVLLIIIFVI
                     NYFTRFRKAHE"
     gene            complement(3673..4686)
                     /locus_tag="AC16CB_01205"
     CDS             complement(3673..4686)
                     /locus_tag="AC16CB_01205"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3341111.1"
                     /translation="MLNKFIKSSEFIIFLILVALSLVVGLINPAFFSISTVFDILRSS
                     IVFFIMAFGLLPIVIAGGIDISFVAIAAVTSYSTHMFLLNRGYQGGILLYYIIACTMG
                     LLAGLLNGFLVTRFKLPVFDVSLAAFTMWYGFTLFFVGATANFDLPSGTVGYYGKFII
                     TAQDPLVGATGLHESFIYVVVIGLLIWWFLNYTTIGRGVFAIGGNREVAVRSGFNITR
                     IMLITFAIMGVLSAIAGVTQAFLSRYFNPVIFITQPLDVLAAVILGGAAITGGRGTVF
                     GTALGVLLIQLINRTLILTGIPVEWQRLVVGIILILFTSLPALRERRAKRIGHTTELT
                     QAE"
     gene            complement(4697..6217)
                     /locus_tag="AC16CB_01210"
     CDS             complement(4697..6217)
                     /locus_tag="AC16CB_01210"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR3341112.1"
                     /translation="MTEEILRVENVSKRFGGVIALDNVSLSIHKGETCCLVGENGSGK
                     STMIKIISGVYTPDEGDIYINGNHYKKLTPIGSINEGIQVIYQDFSLFPNLTVAENIA
                     TNEQLASGKQLVNWSEINRIAKEGLAKINISLPLDAIVSTLSTADRQLIAIIKALLAN
                     ARIIIMDEPTTALTKKEVASLFKLIDELKAQGISTLFVSHKLTEVTEIADRTVIFRNG
                     KKVMDQDAKGLDVQTMEFYMTGRHIDTSAIQFGEVQEAAEPLFRVENLHLSHGFFDLS
                     FELKHNEVLGITGLLGSGRTELALSLFGELPADSGKIFIEGKEVKIKGITDAVRYGIG
                     YVPDDRIREGLFLEQSISNNIVVSVIDRLINKMRLLNMKGKAEESKRWIKQLEVKTPS
                     GELPAKSLSGGNQQRLVLAKWLASKPKILILNGPTVGVDVGSKAEIHELIRSLARQGI
                     GILLISDDIPELLMTCHRILLMRAGRIVEEFKREETTEQELSSELVGAAASPTNSH"
     gene            complement(6300..>7232)
                     /locus_tag="AC16CB_01215"
     CDS             complement(6300..>7232)
                     /locus_tag="AC16CB_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR3341113.1"
                     /translation="EGEEMVFASVVKSIAFNWFLRLETGVLDFGEDYGVKAFMEGPSQ
                     VDSAAQVAIIEDLIAQGVDAICNVPYGVPENEPAQKKAMDAGIIVIGHEAATAKAGTL
                     NYDVEAFDNCAYGEEMMKEVATRMGESGQYIQFVGSLTNASHNEWQDCAKAYADANYP
                     NLEFVAKYESKEDQEVAYNTMKDVLKTYPDVKGVLGSAAGDVVGAGRAIEEAGLQDAV
                     AVVGTSIPSYAGELLKTGAVDLAMAWDPATAGYACNVVAYKLIKGEAITDGMDLGVPG
                     YEKIVIRKGENGVDVIYGAAWIKMDASNMDDYPF"
BASE COUNT         1879 a         1947 c         1727 g         1679 t
ORIGIN      
        1 ctggcataca ggtcgcgaaa gatttgataa ttgatctcat acagcttgcg ggactccagg
       61 ttggggtgga tcgtttcttt gattcgcacc cattgcttaa tctcggacag atccttgaac
      121 aatcccacac cggtggcagc taagaaagca tcgccatagc ttgccccaat ttgttgttca
      181 gggacaacca aatcgatatt acatacatcc gctacaatct gcagccataa cgggtttttg
      241 gtgcctccgc cgacggcaag gatgcgcgct ggttgaacgc cctcctcgcc catgacctca
      301 acattgtgcc ggatgccaaa tgcaactccc tccaaaatag cccggtaaat atcccctttg
      361 gtatgcttga ggctcagacc aaaccagacc cctttcgcct tcggatcgtg caagggggtg
      421 cgctcaccct cgaagtaagg cagagcaatc aggccatttg agccgatggg tgaggcagat
      481 gccaagcttg caagaacagc ataggcattt tccccgccct ctttttcctt ttcgatctcg
      541 tagtgagcca attggtcacg gaaccaggtg gtcagacttc cagaggtgga catcccaccc
      601 aagaaggcaa acgagccttc ctctaaaaag ttggacgacc agaaactctg ggtcttgatc
      661 agctcggcgg ttttcatgat gaagaaaatg ctgctgccga acatcaacat catgtcgcca
      721 aattccgcta ctccagcact gaccgcctcc gcggctgcat ctgttgtgcc cgccaccacc
      781 ggcgtgccct cggcgagacc agtttcttgg gccgcctcgg cggtcacttc tccgacaatc
      841 tcgtaactcc aataggcctt ggggagtcgc tcgaggggag tgattagctc agcggcctct
      901 gatatccact ctcgcgagtg gacatcgtat aaaggagcat accccccagc agtgtagata
      961 tcaatcgaag cttttccggt cagtttgtgc accagatagg cttcgctggt caggaaccag
     1021 cgtgttttct ggtagatttc gggctcgtga ttgcgtatcc acaaaacctt cggcccagaa
     1081 gcttgcgaac tcagatgcga gccgctggtt ctgaatatct catgcttccc caattccctc
     1141 tccagatact cgatttctgt cgaggcgcgt gtatcaatgc catacaggat gcccggacgc
     1201 aatggcttgc cattttcgtc gataggtaat acacacgaac cgattgcgct agtgcctatg
     1261 cccagaatct gtttcggatc gataccactg ctggaaagca gattttttgt gattacgaca
     1321 aaatcatgcc accagacctg gtcggcatcg tgctcaaaat aacccggctt gggcatatcc
     1381 atgccatgcg gcacagtatg gctggcgacg actgtgccat ccgtcttcac tagcacacct
     1441 ttggaagaat acgtgccgat atcaactcca accagaaagc gatttgacat tctccctctc
     1501 tccaagcgag gatctaaaga agcactttgc cattccggaa gacgagctcg tcatccagat
     1561 agatctcgcc ctcctggcgc atgtctttga taatgtccca gtgaatagca gattggttcg
     1621 tgccgcctac ttttggatag gctcgaccca aagcgatgtg aacggtgccg ccaatctttt
     1681 catcgatcaa gatatctttg cagaagtaat tcacttccgg gttcgtgccg atggcgaact
     1741 caccgatcaa gcttgcaccc gcgtccgtat taagcacaga gcgcaggaaa tcctggttag
     1801 tggaggatgt ggcttcaacc aatttccctt gttcccagcg caagcggata tcgtgcacca
     1861 accgccctcc tagcacaccc ggaaaatcga aatagatgac accgtcgatg gtgctttcca
     1921 ccggcgcagt ggcaatttcc ccatccggca tattgctgtg gccagcggcg acatcccatg
     1981 ttcgcccttc ggtggagaag ctcaattctg tccctttgcc gacaatgcgg atttgatccc
     2041 ccttggtcaa ttcatcggcc caacgccgcc actcctggct cacgacaggc caatccagga
     2101 aacaggcgtt gaaaaacata tctgtgattg tctcttcgtc aacaccagcc tggtgtgcca
     2161 gggcggcatt cggcacgcgc agcaaacacc aacgggtttt ttgccaacgc aaggtggaga
     2221 tcttccccat ggcttggcgc aggagcgaga gcttttcggc agggatatcc caaaacacgt
     2281 ccagattatg ggcgccccgt aagccgaaat agacatcggc ccattccatg ccatacgctt
     2341 cgatctccgg cacccagccg atctgctcgg gagttccgtg tacaagtacc agccggttca
     2401 actcttctga gagaaattgg acttgtggga gcgctccagc ctggaggcaa gaccgatata
     2461 tggcgtgtac gagtggatag gtttccactt caacaaaggc gatcatcacc ttttcaccgg
     2521 gtttgaccgc catagagtaa ttcaccaaca aatcacccaa ctgagcccaa cgtttatcca
     2581 tgatcgatgc actcctaacc tagcgtgcgc tttcactcat gggctttccg gaagcgagtg
     2641 aaataattaa tgacaaagat gataatgagc aagacacccc acgagaaatc cttgaagaaa
     2701 gagctgccgc gtatcccaac cagcaccatg tgaaagccgg tggacaggat ttgcaggatg
     2761 agcacggcga taaagatatc gaccacatgc ccaaaccctg gcaaaatgcc cgccagtacc
     2821 gcgatgagga tagtcagcaa cacataggtt gttgaaccat attcatacgc cgcagacatg
     2881 gtgcggctca tgatcaaaat accagtaata gccgaaaaaa cgccgctgac aatataggta
     2941 ttcatgatga tggatttatt gtctatgcca gaaaaattag atgcaatcgg gttcgagcca
     3001 agcatgcgcg ctttgaagcc aaaagttgtc cggttgagga gaaaataagt gtaaatggtc
     3061 aacaggacga aaatcaggaa aggaatggga atcccaaaga atgtctcgtt cccaatcccc
     3121 gagagctgtt caggaaaacc agtcacggtt gcaccaccgg tcaagccagt agatatgccg
     3181 gtgtaaaaag tgagcgtggc aagcgtggct aagatcggcg gcacgccaac atgagcgatc
     3241 aaaaagccat tgatcacgcc actgagccac ccaacgacca gcgcaataag cagcgcaatg
     3301 gcgatataga gcataatctg ctgaggcgag gcatccgggg ggataaactt gaccatgaaa
     3361 aagccagcta gcaccgcggc taggttggag gtcgcaacca ccgagaggtt gatgccgccg
     3421 gtcaggatgc tgagcatcat agcaagcgaa agaacgccca gttcgggaag ctgaaagccc
     3481 atagaggtga cgctgcgcac ggttaagaag cggtcctctg ttatagcgaa gaaggcaaac
     3541 agcacgacga atacgcctag cagcagagcg gtctgactgc ccatctggat tttcttgatc
     3601 aaggtggaaa gacttttcat caatgtggaa gggtttgatt ttgtatccat aatcatgcca
     3661 ttgttaatgt aatcactctg cctgagttaa ttcggttgtg tgcccgattc gtttggcgcg
     3721 gcgttctctt agcgctggaa gcgaagtaaa gagtattaag atgataccca cgaccaagcg
     3781 ctgccattcg actgggatgc cggtcaaaat cagtgtgcgg ttaatgagct ggatgagcaa
     3841 tacacctaat gccgtgccaa agaccgtgcc gcgcccacct gtgatggctg cgccgcctaa
     3901 gataaccgct gccagcacat ccagtggttg cgtaataaag atcactggat tgaaatatct
     3961 actcaagaat gcctgggtaa cccctgctat cgctgagaga acccccatga tggcaaatgt
     4021 gatcagcatg atgcgggtga tattaaaacc cgagcgaaca gccacctcac gattgccgcc
     4081 aatggcgaaa accccccgcc cgatggtggt gtaattcaaa aaccaccaga tcaacaaccc
     4141 gatgacaacc acatagataa aagattcgtg caagcccgta gcaccaacca gtggatcctg
     4201 ggcagtaata atgaacttgc cgtaatatcc gaccgtgccc gaaggtagat cgaagttagc
     4261 agttgctcca acaaagaaga gggtaaaacc gtaccacatg gtgaaggcgg ctaaggatac
     4321 atcaaagacc gggagcttaa acctggtaac caaaaaaccg ttcaataaac ccgctagtag
     4381 ccccatggta caagctatga tgtagtacaa caggatgcca ccctggtaac ctctgttaag
     4441 gaggaacata tgagtggaat acgaagtaac agccgcaatg gcgacaaaag agatatcgat
     4501 gccgcccgca atgacaatgg gtagcaggcc aaaggccatg ataaagaaga caatagacga
     4561 gcgcaggata tcaaatactg tggatatgga aaagaatgct ggattgatta agccgaccac
     4621 caaggacagg gctaccagga tgagaaatat aatgaattca ctgctcttga taaacttatt
     4681 taacatggca caccctctag tgtgaattgg ttggagaggc ggcagcaccg accagttcag
     4741 agcttaattc ctgctcagtg gtctcctcgc gcttgaattc ttcaacgatt cttccagcac
     4801 gcatcaatag aatacgatgg caggtcatca gaagctcagg gatatcgtcg gagatcaata
     4861 agataccgat gccctgcctg gctaaagaac ggatcagctc gtgaatttcc gctttcgaac
     4921 caacatccac tcctacggtt gggccattta agatcagaat cttcggcttg cttgccagcc
     4981 acttggcaag caccaatcgc tgctgattgc caccagaaag gctcttggca ggcaactcgc
     5041 ctgaaggtgt tttcacctcg agctgtttga tccagcgctt cgattcttcg gctttgcctt
     5101 tcatattgag gagtcgcatt ttattaatca gccgatcgat aacactgact acgatattgt
     5161 tgctgataga ttgctccaag aacaagccct cgcggatgcg atcatctggc acgtaaccaa
     5221 tgccatatct gacagcatcc gtaatgccct tgatcttgac ctctttacct tcgatgaaga
     5281 tcttccctga atctgccggc aactccccaa ataaggataa tgccagctct gtccggcctg
     5341 atcccagcaa acccgtaata cctaatacct cattatgttt gagttcgaaa gagagatcga
     5401 agaaaccatg cgagagatgt aggttttcaa cccgaaacag gggttcagcc gcttcctgca
     5461 cttccccaaa ttggattgcg ctggtatcaa tgtggcgacc ggtcatataa aattccatcg
     5521 tctggacgtc cagccctttg gcatcctggt ccatcacctt tttgccattg cgaaagatca
     5581 cggtccggtc ggcgatttcg gtcacctcgg tcaatttatg gctaacaaaa agcgtcgaga
     5641 ttccctgcgc ttttagctca tcaatcaatt taaacaatga agccacttct tttttggtca
     5701 aggcagtagt cggttcatcc ataatgatga tacgagcatt ggccaaaagc gctttgataa
     5761 tggcaatcaa ctggcgatca gctgtggaca atgtactgac gatggcatcc agcggcagtg
     5821 aaatattgat tttcgccaag ccctccttcg caatgcggtt aatctccgac caattgacca
     5881 gttgcttgcc acttgcgagt tgttcattgg tagcgatatt ttcggctacg gtgaggttgg
     5941 ggaaaagaga aaaatcctga taaataacct ggatgccctc attaatcgat ccgatcgggg
     6001 ttaatttttt ataatgatta ccattgatat aaatatcacc ttcatccggt gtataaaccc
     6061 ccgaaatgat cttgatcatc gtggactttc cggagccgtt ctcccccacc aagcagcaag
     6121 tttcaccctt atggatagat aaactcacat tgtccagcgc gatcacccca ccaaatcgct
     6181 tgctgacatt ttcgacacgc aggatttcct ctgtcatgga ctatgcacct tatttattat
     6241 aggatgttta agagtgggcg gctgagatat tcagccgccc actagttcat tatattggct
     6301 tagaatggat aatcatccat gttgctggcg tccatcttga tccacgccgc cccatagatc
     6361 acatccacac cgttctcgcc cttcctgata acaatcttct catatccggg cacaccgagg
     6421 tccatgccat ctgttatcgc ctcgccctta atcaatttgt aggccaccac gttgcaggca
     6481 tatcctgcag tggcgggatc ccaagccata gccaaatcga ctgcgccggt tttgagcaac
     6541 tcgccggcat aggaaggaat gctcgttcca accaccgcaa cagcatcctg caggccagct
     6601 tcctcaatgg cacgaccagc acccaccacg tcgcccgctg cggaacccag gacacctttg
     6661 acatccggat aggtcttgag gacatctttc atggtgttgt aggccacttc ctggtcttct
     6721 ttggattcgt acttggcgac gaactcgagg tttggatagt tggcatcggc ataagctttg
     6781 gcacaatcct gccattcatt gtgggaggca ttggtcagcg agccgacaaa ctggatatac
     6841 tgaccggact cacccatgcg ggtcgccact tctttcatca tttcttcgcc gtaggcacag
     6901 ttgtcgaagg cttcaacatc gtagttgagg gtgcctgctt tggcagtagc agcctcatgc
     6961 ccaatgacaa taatgcccgc atccattgct ttcttctggg ctggttcgtt ctctggcaca
     7021 ccgtaaggga cgttgcagat cgcatccact ccctgagcaa tcaggtcctc gatgatggca
     7081 acctgggcag ccgagtcaac ctgggagggc ccttccataa aggccttaac cccatagtct
     7141 tccccgaaat ctaatacccc agtctccagg cgtaagaacc agttaaaagc gatcgacttc
     7201 accactgagg cgaagaccat ctcctctcct tc
//
LOCUS       DBNGSX010000035         4312 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1110365, whole genome shotgun sequence.
ACCESSION   DBNGSX010000035 DBNGSX010000000
VERSION     DBNGSX010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1110365"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="AC16CB_01220"
     CDS             complement(<1..537)
                     /locus_tag="AC16CB_01220"
                     /inference="COORDINATES: protein motif:HMM:NF018012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR3341114.1"
                     /translation="MTLQAIDHQLPRPYRYSRLTDEQFQLVHSASLEILERIGVRVDL
                     GEALDLLKHAGARVEDNLVRLPPRLVEQALQTAPKQVTLFNRHGEPVMPLDGDRCFFG
                     PGSDCLNIIDHRTGERRKPVMQDVVEGMILCDALPNIDFVMSMFLPADVDQTIADTYQ
                     AEVMLSHTTKPIILVSYGA"
     gene            complement(578..1687)
                     /locus_tag="AC16CB_01225"
     CDS             complement(578..1687)
                     /locus_tag="AC16CB_01225"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3341115.1"
                     /translation="MNETIVELRNVTKIFTSRRGATLEEVQAVKDVSLNIEQGEFFTL
                     LGPSGCGKTTTLRLIAGFEIPTSGEVLIQGNRMSRTPPYQRPVNTVFQNYALFPHLSV
                     LKNVAFGLAVKRMARAQREVLAQEALDLVRLAGMGDRKPSQLSGGQQQRVALARALVN
                     KPAVLLLDEPLGALDLKLRKAMQLELKQLQEQVGITFIYVTHDQEEALTMSDRIAVMK
                     DGIVQQLGDPVTLYERPANRFVADFIGESNLIQALVESISDRRVDLRIGQEQMTASVG
                     DAPPAPGQEIVVAIRPEKLRITAQQEILAQAMSGTVSQVIYIGTDTRYVVDLASGESI
                     TIRVQNSFGRDLEEFKRGDRVRISWAAEDARTLLV"
     gene            complement(1684..2490)
                     /locus_tag="AC16CB_01230"
     CDS             complement(1684..2490)
                     /locus_tag="AC16CB_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3341116.1"
                     /translation="MKRQHSFSPFTLYYWLVTAVLFLPIILLIIFSFNNSITLVFPLK
                     GFTLKWYQQLIQNYELIRSVRTSLLLGIGTSLVATALGAMGAIAMVRFNFPGKSLFIS
                     VAAMPLVIPYVILGVSLLIMFHQVGISLSAFTAGLAHVVVSVPYALLVIASRLVGFPQ
                     NLEEAAMDLGCTYWGALLRVTLPISFPALVAAFLICFTISFDEFAIASFLVGTQPTLP
                     VYLYSQLRFPTRLPLVVSMASILMTGSMILILLSEWLRRLGQTSGGSAKS"
     gene            complement(2491..3342)
                     /locus_tag="AC16CB_01235"
     CDS             complement(2491..3342)
                     /locus_tag="AC16CB_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3341117.1"
                     /translation="MENRSRAAYLVGPPAVYLLFLFILPLGIMAIFSFRAGTIGAASQ
                     QFTLDNYIDFIQNATYHRLLWRSALIAFTISAVSVLFAYPLAYFLVFIAGERRITFLT
                     LIIVPTWTSYLLRIFAWKLILGSQGLLNTLLLWLGLIEQASSILLYSRTAVVITLIYV
                     WVPFVALPIFAALERIDRNLLEAAADLGCRPWEAFLRVTLPLSMPGVVAGFMFALIPT
                     LGEFVTPLLVGGGQGAMYGNLIQDQFVRALNWPMGSVMSLAMLVMVLLIIFAFLRGGS
                     AAKLLDI"
     gene            complement(3429..>4312)
                     /locus_tag="AC16CB_01240"
     CDS             complement(3429..>4312)
                     /locus_tag="AC16CB_01240"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3341118.1"
                     /translation="FSFLTESAEVYSKLQSGFEADLVHPCSNFWKLLVDENLVQPIDT
                     SKLSRWNSVIESLAKEGEFNGKQYWIPWDWGFESILVRKDLVEQVPTSWADIWDPKYA
                     GQIAINDSGEAAHIWASLALGLDPWNQTDEQYAQVRQKLLELAPSVKAYWTGQTDLDQ
                     MLASGDVSLGVGAWNASYVSLLGEGLDVDYIAPKEGRTGYLCGFGIPTSAKNVDLAHE
                     MIDAYLAPESMVYLANEYGYGIVNEDAIPQVDPEMAELLGLEDPNVISQTVFYKSITS
                     EQRELWTNTWSEVKTSQ"
BASE COUNT          987 a         1168 c         1259 g          898 t
ORIGIN      
        1 tgccccgtaa gagaccagga tgatcggctt ggtggtgtgt gacagcatga cctccgcctg
       61 gtaggtgtcg gcgatggtct gatctacatc ggctggcagg aacatcgaca tgacaaaatc
      121 tatattgggt aaggcgtcgc acaggatcat accttcgaca acatcttgca tcaccggctt
      181 gcgccgctcg cccgtacggt gatcgatgat gtttaagcag tctgagccag ggccgaagaa
      241 acagcggtca ccgtccaggg gcatcaccgg ctcgccatgc cggttgaaca gggtcacttg
      301 tttaggtgct gtctgcaggg cttgctcgac caggcgcgga gggagacgca ccaggttgtc
      361 ttccacacgc gctccggcat gcttgagcaa atccaaggcc tctcccaaat ccacgcgcac
      421 gccaatccgc tctagaatct ccaggcttgc agagtgaaca agctggaact gctcgtcggt
      481 gaggcgtgag tagcgataag gcctgggtag ctggtgatcg atggcttgca gtgtcatctt
      541 gaaaatggtt atcctttttt cccttcgtag aaataaatca aaccaacaat gtccgcgcat
      601 cctccgcagc ccaggagatg cgcacgcggt cgcctctctt gaactcttcc aggtctctgc
      661 caaagctgtt ttgcactcga atggtgatcg attcaccgga ggcaagatcg acaacgtagc
      721 gagtatcggt gccaatgtag atcacttgcg atactgtgcc gctcatggct tgagcgagta
      781 tttcttgttg tgcagtgatc cgcagcttct ccggtcggat cgccacaact atttcctgac
      841 ccggtgcggg gggtgcatcc cctacgctgg ctgtcatttg ctcttgccct atgcgcaggt
      901 cgacgcgccg atcagatatc gactcgacga gggcctggat caggttgctc tccccgatga
      961 agtcggctac aaagcggttg gcgggtcgct cgtacagggt gaccggatct cccagctgct
     1021 ggacaatgcc gtccttcatc acggcgatgc ggtcagacat ggtcagcgct tcttcctgat
     1081 cgtgggtgac atagatgaac gtgatgccta cctgttcctg cagctgcttg agctcgagct
     1141 gcatggcctt gcgcaacttg aggtccaatg cccccagcgg ctcgtccaga agcaacacgg
     1201 caggtttgtt gaccagggcg cgcgccagcg ccacgcgttg ctgctgacct ccggagagct
     1261 gggatggctt gcggtcgccc atccctgcta gccggaccag gtcgagcgcc tcctgggcga
     1321 gtacttcgcg ctgggcgcgg gccatgcgct tgactgccag gccaaaagcc acgtttttca
     1381 gcacgctcag atgggggaac aaggcgtagt tctgaaagac tgtgttgacc gggcgttggt
     1441 agggtggggt gcggctcatg cgattaccct gaatgagaac ctccccagat gtcgggatct
     1501 cgaacccggc aatcagccgc aatgtggtgg ttttgccaca accgctggga ccaagcaagg
     1561 tgaagaactc gccctgctca atgttcaggc tgacatcctt cactgcctgc acctcctcca
     1621 aagtggcgcc gcgccgggag gtgaatatct tcgtcacatt gcgtagctcg acaattgttt
     1681 cgttcatgat ttagcacttc caccagatgt ttggccgaga cggcgtagcc actcggacag
     1741 cagaatcagg atcatggagc ccgtcatcag gatagatgcc atcgagacga ccaggggcag
     1801 gcgggttgga aagcgcagct gcgaatacag gtaaactggc agagttggtt gcgtgccgac
     1861 caagaacgaa gcgatggcaa attcatcaaa ggagatggtg aagcagatca ggaaggcagc
     1921 caccagagct gggaagctga tgggcagtgt aacccgcaat aatgctcccc agtaggtgca
     1981 ccccaggtcc atggcggctt cctctaggtt ttgcgggaag cccaccaggc gggaagcaat
     2041 gaccaacagg gcatatggta cgctgacgac cacatgtgcc agcccggcgg tgaaggccga
     2101 gagcgatatg cccacctggt ggaacatgat caatagagaa actcccagaa taacgtatgg
     2161 gataactaac ggcatggctg ccactgagat gaacaagctc ttgccgggga agttgaagcg
     2221 caccatggcg atagccccca tagcccctag tgcggtagct acgagtgagg tgccgatccc
     2281 taacagaagg ctggtgcgca cagatcggat cagctcgtag ttttgtatta gctgctggta
     2341 ccattttagt gtaaaccctt tcagcgggaa taccagggtg atcgagttgt taaacgagaa
     2401 tatgatcagc agaataatcg gaagaaagag gacagcagtc accagccagt aatacaacgt
     2461 gaagggggag aatgaatgct ggcgtttcat ctatatatcc aatagcttgg cggctgaacc
     2521 accgcgcaag aaggcaaaga tgatcagaag taccatgacc agcatagcca ggctcatcac
     2581 cgatcccatt ggccagttca aagcgcgcac gaattggtct tgaatcaggt tgccgtacat
     2641 cgctccttgc ccacctccca ccagcagagg tgtgacgaac tctcccaggg tagggatgag
     2701 ggcgaacata aagcctgcga ccacaccggg catgctgagc ggcagggtga cgcgcaggaa
     2761 ggcctcccaa gggcggcagc ccaggtcggc ggcggcctct agcaaattgc ggtcaatgcg
     2821 ctccagagcg gcgaagattg gcagggcaac gaagggtacc cagacgtaaa tcagggtgat
     2881 cactaccgcg gtgcggctat aaagcaggat ggacgaagcc tgttcgatca aacccaacca
     2941 taaaagcagc gtattgagca ggccttgaga gcccaatata agtttccagg cgaagatgcg
     3001 cagcaggtag ctggtccagg tgggcacgat gatcaaggtt aagaaggtga tccgccgctc
     3061 gccggcaatg aataccagga aataggccag cgggtaggcg aataggacag ataccgcaga
     3121 gatggtgaag gcgatcaggg ccgagcgcca gagcagccgg tgataggtgg cattctggat
     3181 gaaatcgata tagttatcca gggtgaattg ctggctggct gctccaatcg tccctgcccg
     3241 gaagctgaat attgccataa tgcctaacgg taggatgaaa aggaacaaca aatacacggc
     3301 tggcgggccg accaaatagg ccgcccggct tcgattttcc attggtttac tgaatcctct
     3361 ctaggccttt ccagttaggg atcgctgagg cctaactgga gaggcccagg ctgttcaggt
     3421 acagaacgct actgcgaggt cttcacttcg gaccaggtgt tggtccacag ctcgcgctgt
     3481 tcagacgtga tggacttgta gaacacggtc tgcgagatca cattgggatc ttccagaccg
     3541 agcagctcag ccatctctgg gtcgacctgg ggaatggcgt cttcattgac gataccatag
     3601 ccatattcat tggccaggta aaccatcgat tccggcgcca ggtaagcgtc aatcatctca
     3661 tgagccaggt caacgttctt tgcagaggtt gggatgccaa acccgcacag gtaaccggtt
     3721 ctaccctctt tgggtgcgat gtaatctaca tccaggccct caccaagtag gctgacgtac
     3781 gaggcgttcc atgcgcccac ccctagcgag acgtcgccgg aggccagcat ctggtccagg
     3841 tccgtttgcc cggtccagta agccttcacg gagggagcca gctctaatag tttttgcctg
     3901 acctgggcgt attgctcatc ggtctggttc cagggatcca atcctaaagc gagcgaagcc
     3961 cagatatggg ctgcttcccc cgaatcgttg atggcgattt gaccggcgta tttgggatcc
     4021 cagatgtctg cccaggaagt tggcacttgc tcaaccaggt ccttgcgcac caggatggat
     4081 tcaaatcccc agtcccacgg aatccagtat tgcttaccgt tgaactcccc ttccttggcc
     4141 agtgattcga ttacgctgtt ccagcggctc agcttggagg tgtcaatggg ctgcaccagg
     4201 ttctcgtcca ccagcagttt ccagaagttg gagcacgggt gaaccaggtc ggcctcgaag
     4261 ccgctttgca gcttggaata gacttcagcg ctttcagtca ggaagctgaa ct
//
LOCUS       DBNGSX010000036         7566 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_556099, whole genome shotgun sequence.
ACCESSION   DBNGSX010000036 DBNGSX010000000
VERSION     DBNGSX010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7566)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7566)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7566
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_556099"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2636
                     /locus_tag="AC16CB_01245"
     CDS             <1..2636
                     /locus_tag="AC16CB_01245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HIR3341119.1"
                     /translation="DYSQAVHITPRRASARPVNLTLPIERWAEQRAGGTWSELYNTRA
                     LHNRFPALGAGLWYLVLTVLGWVVYPLARLALPGLVDRGYPMARTIGLLLLSYLVWLA
                     GSARIPFTRITISLVFALILLASATVVYRQRGELRQEWRERRGYFLRVEMIFLAFFVF
                     DLLIRIGNPDLWHPWKGGEKPMDFSYFNAVLKSTSFPPYDPWYAGGYLNYYYYGFVFV
                     GVLVKWLGIVPAVAYNLIIPTLFSMIAMGAFSLGWNLFNSRYLFDNRQPFMQGSEPSF
                     FNRISPFIPALAAAFGMAVLGNLGTVRMLFQGYQRIVAPDGVIEGVGVLTRWWWAARG
                     FVRVLSGANLPYSIGDWYWIPSRAIPAPNDVEPITEFPFFTVLYADPHAHLFALPIAL
                     LVLGIALSLVLGRARWKSRLEVVCGFVLAGLAIGALRPTNTWDFYPYLALGSVAMIYA
                     LGLYFRPVAERPLPLIGRLPPSSQRLVVTISGFLLLLALSMFLYQPYATWYALGYSKL
                     AIWKGSHTTLSAYLTHWGLFLFVIVSWMVWETRDWLASTPVSALRKLKPYRELVLSSP
                     FVLLGVVLLLAFYFEVAIAWLVLPLAVWAGVLLLRPNQSDAKRFVLFLVGSGLLLTLM
                     VEVIVLVGDIGRMNTVFKFYLQVWTMFAVCAAAALGWILTDISGWLPGWRVSWQVVLT
                     ILGFGAAMFSLMGSMAKVEDRMSLEAPYTLDGMAFMAHTQYEETWGTMDLSQDYRAIR
                     WMQENVSASPVIVEANQLNLYRWGSRFSIYTGLPGVVGWEWHQKQQRAAIPTNWVSER
                     IAEIETFYTTTDLLQAQSFLSKYDVRYIILGQQERGLYAGEQGEQLEGLAKFPAADGL
                     LWRSVYRDGDTIIYEVMDKGN"
     gene            2638..5208
                     /locus_tag="AC16CB_01250"
     CDS             2638..5208
                     /locus_tag="AC16CB_01250"
                     /inference="COORDINATES: protein motif:HMM:NF021570.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HIR3341120.1"
                     /translation="MLSALLWYFTITLIGWITFPLVYRLLPALVDRGYAFTRTVGLLL
                     WAYAFWLLASLGILRNDLGGLLFSVLLLIALSGWALRSVSLEEMADWLRKQRSLVLVV
                     EILFLVAFAFMGLVRAANPEILGTEKPMELAFINAILNSPEFPPHDPWLSGYAISYYY
                     FGYVMVAMLAKLTGTPGGVAFNLGVTLVFALSAVGSYGLVYNLLSFRSTRLPRRQMES
                     SGRVAQAKSTFLSLALLGPIFVLLIGNLEGFLEVLHARGLFWQSTPTGELTSPFWKWL
                     DMRELSSPPQQPFSWTPSRYLWWWRASRVVQDYDFAENWKEVIDEFPAFSYLLADLHP
                     HVLAMPFAFLAMGLALNLFLGGGEGRLSWLQRGLGERVESWLSLEINPPVFLLGAVCL
                     GGLAFLNTWDFPFYVALFAGAFALVRMRQVGTGWGVGISEFFRLTIALVVTGIILYLP
                     FYIGFSSQAGGVLPNLVYPTRGTHLWVMFAPLLLPLFAFLYFRWMHSSGLGFWKGGAL
                     ALSLTLLLWLLSILLTVGIAMLPEVNNLFLGSLSAPDYGNLLAESLVRRIRSPGGWLT
                     LLILLAMTLGLMLKAVGEKKIEENVEGSRALSNRESAQDVPQSLSPNIFVLLLILLGT
                     LLVLGPEFLYLRDLFGWRINTIFKFYYQAWLLWAVAAAYASSILLLIPRRPWNLIYRG
                     VLILIMMAGMTYTSLGLWTKTNAFKPAMGLTLDGTAYLLNQSPNEMSAIAWLSAAPAG
                     VVAEAVGGSYSIYGRVSTLSGQPTVLGWDFHETQWRGSSLLLGTRQNDIQRLYCTRDW
                     NEAQEIINLYNIRYIFLGNLERSDFTPANCPGGLNDVKFIRNLAVAFQQGEVTVYMVP
                     "
     gene            5341..7092
                     /locus_tag="AC16CB_01255"
     CDS             5341..7092
                     /locus_tag="AC16CB_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341121.1"
                     /translation="MSFNRLSIEQVLYGLAILLALTVRLLNLGAIPLADSEAVLALQA
                     FDVSQGEKVAIDPQPGFAALTGLTFALLGSSEFTARLWPALAGGLLVIVPYFLRRQLG
                     RGAAIILAFGLAFDPGLVAVSRQADGPMLSVGFSLLVLGLAIAGYPILAGVAAGLALL
                     SGPALLPGLLGLALAWGASLLFSDTEEGDQLLPAAWDEKALRKGVLAMLGTLVLVGTL
                     FLRAPQGLGGWIAGLPSYLSGWAQPSGVPALRLVSALIVYQPLAVIFGLIGAIRGWLR
                     GKRLVKVASLWLVFALILSILYPARQVHDLIWALIPLWILAALTLAGLFADSGENSMI
                     SLGHATILFIFMTLVWVNIAGLGQSEPGSPGYLTRLAVVGGVIALGAVTSGLIGLGWS
                     WKTAQNGAVWGMCAILGIYTLAGMWWASQVREPGRVELWQPFPSSAGTRLITETVTDL
                     SAWNIGDTQALDVTLAVDSPALHWSLRTFPSLSVIPEGEQLISMGSPAIVITRQDVEE
                     PGLAAEYRGQDFVFRTYPGWFGALPPNILRWVAFRLAPLQEEQVIVWARADLFPGGSS
                     LSEESPPAFDIPGLDEP"
     gene            7097..>7566
                     /gene="cmk"
                     /locus_tag="AC16CB_01260"
     CDS             7097..>7566
                     /gene="cmk"
                     /locus_tag="AC16CB_01260"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIR3341122.1"
                     /translation="MPNPTVIAIDGPAASGKSTLGQNLAGELGFLYFDTGVMYRAVTW
                     AALQRNISVHDELAIVTLAESLTIDVRPPSMADGRAYDVWVEGKDVTWHIRRPEVEAD
                     VSEVSAYAGVRRALTLQQRNIGLRGRVVMVGRDIGTVVMPEAALKIYLDASAEER"
BASE COUNT         1385 a         1870 c         2190 g         2121 t
ORIGIN      
        1 ttgattactc tcaagccgtt catatcacac caaggcgggc aagcgcgcgg cctgttaatc
       61 tgaccctacc cattgagcgt tgggcagagc aacgcgccgg aggcacctgg tcagagttgt
      121 acaatacccg cgccctgcac aaccgattcc cggctctagg tgcaggacta tggtatctgg
      181 tcttgaccgt tttgggttgg gtggtttatc cgctagcccg tctggcgtta ccagggctgg
      241 ttgatcgcgg ctacccgatg gcgcgtacca tcggcctttt gctactcagt tacctggttt
      301 ggctggctgg ttctgctcgc attcccttta cccgtataac tatcagtctg gttttcgcct
      361 tgatccttct ggcaagcgcc acggtggttt atcgccaaag gggtgagttg cgtcaagagt
      421 ggcgggagcg ccgaggctat ttcctgcggg tagagatgat cttcctggca ttttttgttt
      481 tcgatctctt gatccgtatt ggtaatcctg atctgtggca tccttggaag ggcggcgaga
      541 aacccatgga tttctcgtat ttcaacgcgg tgttgaagag cactagcttc ccaccttatg
      601 atccctggta tgctggcggg tatctgaatt attactatta cggctttgta tttgtgggtg
      661 tgctggtcaa gtggttgggg atcgtcccag cggtagccta caatctcatt atccctacgc
      721 tgttttccat gattgccatg ggcgcctttt cgctggggtg gaatttgttc aattctcggt
      781 atctgttcga taatcgccag ccatttatgc aaggcagtga gccatcattt ttcaatcgta
      841 tctcgccgtt catccctgcc ctggcagctg cttttgggat ggcagtgcta gggaatcttg
      901 gaactgtgag aatgcttttc cagggatatc agcgcatcgt cgcaccagat ggcgtgatcg
      961 aaggcgttgg tgtattgacg cgctggtggt gggcagcgcg cggttttgtg cgcgtgctct
     1021 ctggggcgaa cttgccctat tctatcggcg actggtattg gatacccagt cgtgccatac
     1081 cggctcccaa cgacgtggag ccgataaccg agtttccgtt ctttacagtg ctatatgctg
     1141 atccccacgc acatctcttt gctttgccca tcgccctgct ggtattgggg atcgccttgt
     1201 cgctcgtatt agggcgcgcc cgctggaaaa gcagactgga agtagtgtgt gggttcgtcc
     1261 tggcgggtct agccattggc gcgcttcgac ctaccaatac ctgggatttt tatccttacc
     1321 tagcgctggg aagcgtagca atgatatatg cccttgggct atatttccgc ccggttgctg
     1381 aaagaccatt accgctgatc gggcgattgc cgcccagctc ccagcggctt gtggttacta
     1441 tcagtggctt cttgttgctc ttggctctct caatgtttct ttaccaacca tatgctacct
     1501 ggtatgcgct gggttactcg aagctagcca tttggaaagg cagccatact accctgagtg
     1561 cttacctcac ccattggggc cttttcctgt ttgtgatcgt ctcatggatg gtttgggaaa
     1621 cgcgcgattg gctggcttcc acacctgtct cggccttgcg caagctcaaa ccatatcggg
     1681 aattggttct gtcgtcaccc tttgtgcttt tgggtgtcgt tttgctattg gctttctatt
     1741 ttgaggtggc gattgcttgg ctggtattgc ccttggcagt ctgggcgggt gtgttgctct
     1801 tgcgccccaa ccaatcagat gccaagcgct ttgtcctctt cttggtggga agcggcctgc
     1861 tgctgactct aatggtagag gttattgtcc tggtcggcga cattggtcgc atgaatacag
     1921 tgtttaaatt ctacttgcag gtgtggacga tgtttgccgt ctgcgctgca gctgcactgg
     1981 gctggatatt aacggatata tccggatggc taccgggatg gcgggtttcc tggcaggtgg
     2041 tcttgacgat cctgggtttt ggagctgcta tgttctctct gatgggcagc atggcaaagg
     2101 ttgaggacag gatgagctta gaagcgccat acaccctgga tggaatggcc tttatggctc
     2161 atacacaata cgaagaaacc tgggggacga tggacctcag ccaagattac cgcgccatcc
     2221 gatggatgca ggagaacgtc tccgcctcgc cagtgattgt tgaagcgaac cagctcaacc
     2281 tttatcgttg gggatcgcgc ttttcgattt ataccgggtt gcccggtgtc gtggggtggg
     2341 aatggcacca aaagcaacag cgcgctgcca tacctacaaa ctgggtcagc gagcgtattg
     2401 ctgagattga gaccttttat acgacgacag acctgttaca ggcacagagc ttcttgagca
     2461 agtatgatgt gcgttacatt attctgggac aacaagagcg cggtttgtat gccggtgaac
     2521 agggcgagca attggagggc ttggcaaagt tcccagccgc agatggattg ttgtggcgca
     2581 gcgtgtaccg tgatggagat acaataattt acgaggtcat ggataaagga aattaatatg
     2641 ctttccgccc tcctctggta tttcactatc acgctgatcg gctggatcac tttcccactt
     2701 gtgtaccggc tgcttccagc actggtggat cgaggatacg ccttcacgcg cactgtggga
     2761 ttgttgcttt gggcgtatgc cttttggttg ctggcttcgc tcggaatttt gcgcaatgat
     2821 ctgggtggac tattgttctc tgtgctgctc ctcattgccc tgagcggatg ggcattgcgc
     2881 agtgtttcgc tggaggaaat ggccgattgg ctgcgcaagc agcgatcttt ggtattggtg
     2941 gtggaaatcc tatttctggt ggcatttgct tttatggggt tggtgcgggc ggcaaatcct
     3001 gagattctgg gcactgagaa acccatggaa ctggccttca tcaatgcgat cttgaactcg
     3061 cctgagtttc ctccacatga cccatggctt tctgggtatg caatttctta ttattacttc
     3121 ggctatgtca tggtcgccat gctggctaag ctaactggca cacctggcgg cgtggcgttt
     3181 aacctggggg tcaccctggt ctttgccttg agcgccgttg ggtcttacgg attagtatat
     3241 aacctgctgt cttttcgctc tacccggttg ccccgtcggc agatggaatc gtcagggcgc
     3301 gtagcccagg ctaaatccac cttcctgtct ttggcattat taggtccgat atttgtcttg
     3361 ctgatcggta acctggaagg cttcctggaa gtgctgcatg cgcgcgggct gttctggcag
     3421 agcacaccca ctggtgagct cacttcgcct ttttggaagt ggctcgacat gcgtgagctg
     3481 agctcgcccc cgcagcaacc tttctcctgg acgccctccc gctatttgtg gtggtggcgt
     3541 gcttcgcgcg tggttcaaga ttacgatttc gctgaaaatt ggaaagaagt cattgacgaa
     3601 ttcccagcat tctcctatct gctggcagac ttgcaccctc atgttttggc tatgccattt
     3661 gccttcctgg cgatgggact ggcgctcaat ctatttttgg gtggagggga ggggcgcctg
     3721 agctggttgc agcggggatt gggcgagcgc gtagagtcat ggttatccct ggagatcaac
     3781 cctccagtat ttcttctggg tgcagtctgc ctgggtggat tggcattctt gaatacctgg
     3841 gatttcccct tttatgtagc cctgttcgct ggggctttcg ctttggtgcg tatgagacag
     3901 gtaggcacag ggtggggagt tggaatcagt gaattcttcc gcttaaccat cgcattggtc
     3961 gttacgggga tcatccttta cctgccattc tacatcggct tttcatctca ggccggggga
     4021 gttctgccaa acctggtata tccgacgcgc gggacgcatc tttgggtcat gtttgcccca
     4081 ctgctgttgc cattgtttgc ctttttgtat tttcgttgga tgcactcctc tgggcttggt
     4141 ttctggaagg gtggtgcgct ggcattgagt ctcacattac tgctgtggct tctctccatc
     4201 ttgctgacgg tggggattgc catgttgccc gaagtgaaca atctgtttct gggtagcctc
     4261 tcggcgcccg attatggcaa tctgctggcg gaatcgctgg tccgccggat caggagccca
     4321 ggcggttggc tcacactgct gatcctgctg gcgatgaccc tggggctgat gctcaaggcg
     4381 gtaggagaga agaagatcga ggagaatgtg gaaggcagca gggcgttgag caatagagaa
     4441 agtgcccagg atgtgccgca gtcactttca ccaaatattt ttgtcttgct gctgatcctg
     4501 ctggggacac tactcgtcct cgggccggaa ttcctctatt tgcgcgatct gtttgggtgg
     4561 cgcatcaaca ccatatttaa attttattac caggcctggc tgctctgggc agttgcggct
     4621 gcttacgcct catcgatact cttgctcatc cctagacgtc cttggaacct tatataccgc
     4681 ggcgttttaa tcctgatcat gatggctggg atgacgtaca cttcccttgg cttgtggacg
     4741 aagacgaatg ctttcaaacc tgcgatgggt cttaccctgg atggcacagc ttatttatta
     4801 aaccagtcgc ccaacgagat gagcgcaatc gcctggctga gcgctgcacc tgcaggagtg
     4861 gttgcggaag ctgtgggggg cagttatagc atctatggtc gtgtatcgac cctcagcggt
     4921 cagccaacgg tgttaggttg ggatttccac gaaacgcaat ggcgcggcag tagtttactg
     4981 ctgggaacac gccaaaacga tatccagcgc ctgtattgca cccgtgattg gaacgaggcg
     5041 caggagatca tcaacctgta taatatccga tatatcttct tggggaactt ggagcgcagc
     5101 gatttcacac cagccaattg ccccggtgga ttgaacgacg tcaaatttat tcgcaacctg
     5161 gctgtcgctt tccagcaggg tgaggtcacg gtctacatgg tgccttagga gatttattca
     5221 ttgctggaac cacttccccc aagcgtatga agggtaccag atgccccgcc tgtttttcag
     5281 tataatttaa atcgcttgta tgacatattt ttgactatct aattcaatgg ctgattgatt
     5341 atgagcttca accgtctatc catcgaacag gtgctctacg ggctggcaat tctgctcgca
     5401 ttgacagtgc gattattaaa cttgggagcg ataccgcttg ctgacagtga ggcggtatta
     5461 gcgctccagg cttttgatgt atctcagggc gagaaggtgg ctatcgatcc tcagcctggg
     5521 ttcgctgccc tcactgggct aaccttcgcg ttattaggga gcagcgagtt cactgcccga
     5581 ctatggcctg ccctagcagg tgggctactg gtgattgtgc catatttcct gcggcgccag
     5641 ttagggcgag gagcagcaat catcctagcc ttcggcctgg ctttcgaccc tggtttggtg
     5701 gcagtctcgc ggcaagcgga tggtccgatg ctgtctgtag gctttagttt gctagtgttg
     5761 ggtcttgcca ttgccggata tccgatcctg gctggcgtgg ctgccgggtt ggctttgctc
     5821 tccggaccgg cgctgctccc tggcctgctt ggcctggctc ttgcctgggg agcgagcctg
     5881 ctattctcag acacagaaga gggggatcaa ttgcttcctg ctgcgtggga tgaaaaagcc
     5941 ctgcgcaagg gtgtgctcgc gatgcttggg acgcttgtgc tggttggtac cttatttctc
     6001 cgcgccccgc aaggtttggg cggctggata gctggattgc cgtcgtattt atctgggtgg
     6061 gcgcagccgt cgggcgtacc tgcccttcgc ctcgtgagtg cgctgattgt ctaccaacca
     6121 ctggcggtga tcttcggatt gattggggca attcgaggat ggctgcgtgg taagcggctg
     6181 gttaaggtcg ccagtttgtg gctggttttc gcattaattt tatcgatact ttaccctgcc
     6241 cgtcaggtac acgacctgat ttgggctctc atccctctat ggatactagc cgccttgaca
     6301 ctggctggat tatttgcaga ttcgggcgaa aattctatga tatcgctcgg tcacgccacg
     6361 attttgttta tttttatgac attggtctgg gtaaacattg cgggattggg ccaatctgaa
     6421 ccaggctcgc cgggttatct gacccggcta gcagtggttg gtggtgtcat cgcgctgggt
     6481 gcggtgacat caggcttgat tggcctgggg tggtcttgga agacggctca gaatggcgcg
     6541 gtttggggta tgtgtgccat tttaggcatc tatacactgg ctggtatgtg gtgggcctcc
     6601 caggtgcgtg aacctgggcg ggtggagctc tggcagccgt tcccaagcag cgctggtacg
     6661 aggctcatca ccgaaactgt gactgattta tctgcctgga acatcggtga tacgcaagct
     6721 ttggacgtga ctctggcagt agattcgcct gcccttcact ggtcgctgcg aacatttccc
     6781 tctttgagcg tgatcccgga aggtgaacaa ctgatttcca tgggctcccc cgcaattgtt
     6841 atcacccggc aggatgtgga agaaccaggc ctggctgccg aatatcgagg tcaggatttt
     6901 gttttcagga catatcccgg ctggtttggg gcattacctc ctaatatttt gcgctgggtt
     6961 gcttttcgcc tagcgccact gcaagaggag caagtgatcg tgtgggcgag ggccgactta
     7021 tttccgggtg gttcatcgct ctcagaagaa tctccacctg cctttgacat tcccggtctc
     7081 gatgaaccct agaagaatgc ccaatcccac ggttatcgct attgatggtc cggcagcttc
     7141 aggaaaatcc actctggggc aaaatctagc tggtgaactg ggttttttat actttgatac
     7201 gggcgtaatg taccgcgctg tgacctgggc tgctttgcag cgaaatatct cagtacatga
     7261 tgaattagcc atcgtcacct tagcagaaag cctaaccatc gatgtgcgac cgccttctat
     7321 ggccgatggg cgagcttatg atgtttgggt agagggtaag gatgttacct ggcatattcg
     7381 ccgacctgag gtggaagccg atgtgtccga ggtttctgct tatgcggggg tgcgtcgcgc
     7441 tttgactctc caacagcgta atattggatt gcgcggccgg gtagtgatgg tcggtcggga
     7501 tatcggcacg gtagtaatgc cagaagctgc cctgaaaatt tacctggacg cttcggcgga
     7561 ggagcg
//
LOCUS       DBNGSX010000037         2634 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_833368, whole genome shotgun sequence.
ACCESSION   DBNGSX010000037 DBNGSX010000000
VERSION     DBNGSX010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_833368"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="AC16CB_01265"
     CDS             complement(<1..818)
                     /locus_tag="AC16CB_01265"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR3341123.1"
                     /translation="MKAVLFNQHGGPEVLEYTDFDTPKPGAGQALVWLRAAALNRMDL
                     WVREGWPGIKLEYPHISGADGAGEIAALGDGVSGWSLGDQVVINPSLNCGHCEFCLAG
                     QDNLCNKWALLGETVRGTLAEYVIVPSTNLFSLPADYDPHQAAAAALVFQTAWHSLIT
                     RGGLRPGETVLVVGASGGVNTAAIQIAKLAGAVVYVVGSSEDKLALAESIGADYLIDR
                     SKDENWSKAIFHMTNRRGVDVVVDNVGITFPQSFRTARKGGRILTVGNTGGPKF"
     gene            complement(954..>2634)
                     /locus_tag="AC16CB_01270"
     CDS             complement(954..>2634)
                     /locus_tag="AC16CB_01270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR3341124.1"
                     /translation="FKARYAWAGVLLVAVTPAWFLHSRTAFETVLTTSFYALFLLFDL
                     LYYTESPRYLYAAVIFGAAAFYSYSNAQLIILAAATLLFISDVRYHLRNREVVLRGLL
                     LATVLAIPLIEFRLSHPQAIHEHLRVIGSYWTQAIPLQNKLLMFSQKYLYGLSPQYWF
                     LPNSQDLPRHRMAGFGQMQTLVFPLVLLGLAVCIAKWRSSPHRAVILAALATPVGAAL
                     LDIGVPRVLAFIIPANLLAAIGLEWIVARWKERLPYRPIAVILFLGLSWANFAMLRTA
                     LTQGPLWFQDYGLYGMQYGARQVFEEAIPEYLAKDPEAQILVSSTWANGAENFLQFFI
                     TPQELHRVRMDGIDTYLFRKLPLDDRMIFVMTALEYQKASQSPKFNMVTAEKIIPYPD
                     GSPGFYFTRLRYADNVDAIFAAEQEARRQLVTGPVSLDGKLVELRYSQIDMGEPALIF
                     DNDPFTLMRGLEANPFILEFIFPQPRHVGGVTADLGLINSTLTAKLYTDLDAEPATFA
                     SVFPSANNGDPNIEMPLPDAPERVSKVRIEILDVASGERANIHIRELHLLP"
BASE COUNT          617 a          707 c          738 g          572 t
ORIGIN      
        1 tcaaactttg ggccgccagt attgccaaca gtcagaatgc gcccaccctt gcgcgctgtc
       61 cgaaagctct gcgggaaggt gatgcccaca ttatccacca caacatccac accgcgccgg
      121 ttggtcatat ggaatatggc tttagaccag ttctcgtcct tggagcggtc aatcagataa
      181 tctgccccta tagactccgc aagagccagt ttgtcctcgc ttgagcctac cacgtagaca
      241 acggctcccg ccaatttggc aatctgaata gctgcggtgt tcacccctcc cgaagctcct
      301 accaccagaa ctgtctcacc tgggcgcagc ccgccgcgcg tgatgagcga gtgccaggcg
      361 gtttgaaaga ccagcgctgc tgcagcagcc tggtgcgggt cgtagtcagc gggaagcgag
      421 aagaggttag tgctcggaac aatcacatat tccgccaatg tgccgcgcac cgtctcgccg
      481 agtaaagccc atttgttgca caggttatct tgaccagcca ggcagaactc gcaatgtcca
      541 cagttcaggc tgggattgat cactacctgg tcgccaagag accatcccga gacgccgtcg
      601 cccagggcgg cgatttctcc agcaccatcc gccccgctga tatgagggta ttcgagtttg
      661 atgcctggcc agccttcccg cacccaaaga tccatgcggt tcaatgcagc cgccctgagc
      721 cataccagtg cttgtcctgc tcctggcttg ggggtgtcaa aatcagtata ttccaggact
      781 tcaggacccc cgtgttgatt aaaaagaaca gctttcaagt aatttctcct gaatgacaaa
      841 caggttttct gcaaccatac ccaactctcc catataggat aaagctacct ctatacactt
      901 ggccactctg ccttactcgt tgaaagtcgg gacgaagaca atcacgcttg ggattaaggg
      961 agcaggtgca actcccggat gtggatgttg gccctctctc cgctggctac gtcgaggatt
     1021 tcgatgcgaa ctttggaaac tcgctcggga gcatctggca atggcatttc gatatttggg
     1081 tcgccgttgt tagcgctcgg aaatacactg gcgaacgttg ccggttctgc gtccaggtca
     1141 gtgtaaagct tggcggtcag ggtactgttt atcaatccca aatccgcagt caccccgcct
     1201 acatggcgtg gttgagggaa gataaactcc agtatgaacg ggttggcttc taaaccgcgc
     1261 ataagggtga agggatcgtt gtcaaaaatg agcgcgggct ctcccatatc gatctgcgaa
     1321 tagcgcagct caacaagctt accatccaag cttacagggc cggtcaccag ttggcgcctg
     1381 gcttcctgtt cggcagcgaa aatggcatcg acgttatcgg cgtatctcag gcgggtgaag
     1441 taaaatcctg ggcttccatc gggatagggg atgattttct ctgccgtcac catattgaac
     1501 ttagggcttt gcgaggcctt ctggtattcc aaggctgtca tgacgaagat catgcggtca
     1561 tctaggggca gtttacggaa aaggtaagtg tcgattccat ccatgcgcac ccggtgtagc
     1621 tcttgaggag taataaagaa ttgtaggaag ttctctgccc cattagccca ggtggaagag
     1681 accaagattt gcgcttcagg gtctttcgct agatattctg gtatagcttc ttcgaagacc
     1741 tgtcgggctc cgtactgcat gccgtacaag ccgtagtcct gaaaccacag tggtccttgt
     1801 gttaaggcgg tgcggagcat cgcgaagttg gcccaagata gtcccaggaa caggatcaca
     1861 gcaatggggc ggtagggcag tctctctttc cagcgagcga ctatccattc cagcccgatg
     1921 gctgccagca ggttggcggg gatgatgaac gccagtacac gaggaacacc gatatctaat
     1981 aacgctgccc caacaggggt agcaagcgct gccaggataa ctgcccggtg gggtgaggag
     2041 cgccatttcg cgatgcacac tgccagtccg agcagcacca gcggaaacac gagggtctgc
     2101 atctgtccga agcctgccat gcggtggcgt ggcaggtctt gggagttcgg caggaaccag
     2161 tactgtgggc tgagaccgta caggtatttc tggctaaaca taagcagttt attctgcagt
     2221 ggtatggctt gtgtccagta ggagccaatc acgcgaagat gctcgtggat cgcctgcggg
     2281 tggctcaatc gaaattcaat caacgggatc gctagcacgg ttgctaagag cagccccctc
     2341 agcaccactt ctcgattgcg caggtggtag cggacatcgg agatgaagag taacgttgct
     2401 gcggccagga taatcagctg ggcattagaa tacgagtaga acgcggctgc cccgaagatt
     2461 acggcggcat agagatagcg cggcgactcg gtgtaataca gcagatcaaa caacagaaaa
     2521 agggcgtaga aggaggtggt caatactgtc tcgaaggctg tgcgagagtg taaaaaccag
     2581 gctggggtca ccgccaccaa cagcacgccc gcccaggcat agcgtgcctt gaag
//
LOCUS       DBNGSX010000038         2346 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_902615, whole genome shotgun sequence.
ACCESSION   DBNGSX010000038 DBNGSX010000000
VERSION     DBNGSX010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_902615"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1148)
                     /locus_tag="AC16CB_01275"
     CDS             complement(114..1148)
                     /locus_tag="AC16CB_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR3341125.1"
                     /translation="MAVTIKDVARRLNLSITTVSRALDGYDDVAEETRRKVIQTAREM
                     GYVPNRAARQLRKQRTETIGYILPTSAPRFSDPFFSEFISGLGDEAAVQGYDLLISSA
                     PPGEDAERLVYKRWVHGRRVDGYVLNRMRLRDWRVQFLSQMNAPFVALERTLDPVDYP
                     SVEVDGQAGIRDLMDFLIGKGHQRIAYIGTSPDMAIQADRYAGYRQSMQDAGLAFEQE
                     LVIEADLTRRGGYRAAQKLLSMQDLPTAIVCINDLTAIGALQCAHELGLKTGKDVAIA
                     GFDGIEEAENSLPPLTTLTQPVYEIARELVKILSAEINGTPLARRRIQIQPELILRAS
                     TGAVVSRLGS"
     gene            complement(1243..1455)
                     /locus_tag="AC16CB_01280"
     CDS             complement(1243..1455)
                     /locus_tag="AC16CB_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341126.1"
                     /translation="MSDNSDLIEEILVTIRDGFFGWIAGDPHRRVFNPAPAVAEQRAW
                     LERNSGEIRLSYTAYDWGLNIERDSE"
     gene            complement(1511..2020)
                     /locus_tag="AC16CB_01285"
     CDS             complement(1511..2020)
                     /locus_tag="AC16CB_01285"
                     /inference="COORDINATES: protein motif:HMM:NF019533.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HIR3341127.1"
                     /translation="MDSLQAFRAEKDQFFAHHPQSPLTREQKANFQGLDYFPETPDLR
                     LEVNVEEFAEKNHIEMQTTTGDVQSYIRFGKIHFQVDGQDAELTVYANQHGFFLPFVD
                     SLAGKETYPAGRYLEPELQHNGKLLIDFNMAYNPYCAYNEMWSCPLTPFENRLKVPIR
                     AGERVFQSH"
BASE COUNT          616 a          629 c          511 g          590 t
ORIGIN      
        1 tctcacttcc tcctttcaat tactcaacct agagcccctt ggtgcccgat acgttttgat
       61 taagttataa aaaccgaaat ccctcaagcg tgcgcagttg aaagaaaaca gttttaactc
      121 cctaaccgtg aaacaactgc tccggttgaa gcacgcaaga tgagctctgg ctgaatttgg
      181 atacgccttc tagccagggg cgttccgtta atctcggcag acaaaatctt cacaagctcc
      241 ctggcaattt cgtaaacagg ctgggtaagc gttgtcagag gcggtagcga attctcagcc
      301 tcttcaatgc catcgaagcc tgctattgca acatcctttc cggttttcaa tcctagctca
      361 tgggcgcact gcagtgcacc gatggcagtc agatcgttga tacacacgat agctgtgggt
      421 aggtcttgca ttgataacaa tttttgagcc gctcgatatc ctccccgtcg agttagatcg
      481 gcttcaataa ccagctcttg ctcgaaggca agcccggcat cttgcatgct ttggcgataa
      541 cctgcatacc gatcggcctg gatagccata tctggggatg tgccaatgta agcgatacgc
      601 tggtgccctt ttcctataag aaaatccatc aagtctctaa ttcccgcctg accgtctacc
      661 tcaacggatg gataatctac agggtctaat gttctctcaa gcgcaacaaa aggggcattc
      721 atctgggaca gaaactgaac ccgccaatct cgcaagcgca tacggttgag gacatatccg
      781 tccactctcc tcccgtgaac ccagcgtttg tacaccaaac gctcggcatc ctcacccgga
      841 ggagcagaag aaattaataa atcatagccc tgaacggcgg cttcatcccc cagacctgag
      901 ataaattcag agaaaaaagg atccgaaaaa cgcggggcgc ttgtcggcaa gatatacccg
      961 atcgtctccg tgcgctgttt gcgcaactgg cgcgccgccc gattggggac atagcccatc
     1021 tcgcgcgctg tctggatcac tttgcgacgt gtctcctcgg cgacatcgtc ataaccgtcc
     1081 aaggcgcgcg aaacggtcgt aatcgataaa ttcaatcgcc tagcgacatc cttaattgtg
     1141 acagccatag tccgttttcc aaaacgtttt ggatgattta tataatatca aacaatatat
     1201 cgaaggtcaa gcattttggc ttctaaaatg ctccaaaacc ccctattctg aatcgcgctc
     1261 gatattcaat ccccaatcat atgccgtgta gctcaggcgg atttccccag aattcctctc
     1321 cagccaggcg cgctgctcag caaccgctgg agctggatta aacacccgcc tatgaggatc
     1381 gccagcgatc caaccaaaga atccatccct tatcgtcacc aagatttcct caataagatc
     1441 gctgttgtca gacattttgg tatcaccagg taagtaaccc cggggcacag gcgaatcatc
     1501 gatcgcacaa tcaatgagac tgaaaaaccc gctcgccagc gcggataggc accttcagcc
     1561 tgttttcgaa tggggtcaga gggcatgacc acatctcgtt ataagcgcaa tacggattgt
     1621 acgccatgtt gaaatcaatt aacaatttgc cattgtgctg tagctcgggt tccagatagc
     1681 gcccagccgg gtaagtctcc ttacccgcca agctgtccac gaacggcaaa aagaaaccgt
     1741 gctggttggc gtaaacagtc agctcggcgt cttgcccatc cacctggaaa tgtattttgc
     1801 caaagcgaat gtacgattga acatctccag tagtggtctg catctcgatg tggtttttct
     1861 cggcaaattc ctccacattc acttccaaac gcagatcagg agtttccgga aagtaatcca
     1921 agccttgaaa gttagccttt tgctcgcggg ttagcgggct ttgggggtga tgggcgaaaa
     1981 attgatcctt ctcagcacga aaagcttgca aagaatccat gcaaatcctc catacagaat
     2041 acttatttag gttaaagacg ccgtattaaa tgctcgtcta acttggccag atatctttat
     2101 caactactaa tattagcctg tgcaaatctc agtatacatc tcataggtta tattctacac
     2161 ttgtgattga aattactagt ataggagaca ttgttggatg cctgagcaga cgccgcgcac
     2221 attgagccat ttcgcttttt atcggtttag caaacaactc taccttctcc cctcagacga
     2281 acgcaaacaa atcctttctg catggttggc tctgatttca gccactgctt caaaagtgga
     2341 cgtata
//
LOCUS       DBNGSX010000039         9926 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1041267, whole genome shotgun sequence.
ACCESSION   DBNGSX010000039 DBNGSX010000000
VERSION     DBNGSX010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9926)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9926)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9926
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1041267"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..815
                     /locus_tag="AC16CB_01290"
     CDS             39..815
                     /locus_tag="AC16CB_01290"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HIR3341128.1"
                     /translation="MVNSFMNILAFFAHPDDETVLIGGTLALLARNGARLHFLCATRG
                     EGGETGEPSLCSEEELGNEREKEMVCAVQNLGGSSLTFLDYVDPRVGPDEQLYAYTQD
                     LTLLAGQVATSIRQFEVDAVITHGSNGEYGHPAHVLTHQAARIAVESFGKGHPLLYTV
                     AANFPDHPRPRLANRDDPAHLVLDISPAMSEKTQAALCHRTQHALFVRRSSQEAGRQL
                     TVPEVIQHLESLHRALPPTNGGVQDELARLLKPWEAHGTA"
     gene            855..1595
                     /locus_tag="AC16CB_01295"
     CDS             855..1595
                     /locus_tag="AC16CB_01295"
                     /inference="COORDINATES: protein motif:HMM:NF013355.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/alpha barrel domain-containing protein"
                     /protein_id="HIR3341129.1"
                     /translation="MKFDLVFPTPLMNAAGSLGFAPDVRRMASLGELGAFVTNPVSLS
                     PRSPAQSRGLIAFSGGFLLHSGYPNPGLKAVIRRYALRWARADMPVIVHLLAQGEDEI
                     SSMVSRLENVDGVSAVEIGLPPDTSKELAIRLIQAGIGELPVIARLPLERAQELAELV
                     SQAGTAAVSLSPPRGAIPAPDGGLLRGRLYGPAIYPQALLVVQALARSGIPVIGGGGV
                     YRHADAEAMLSAGALAVQLDSVLWRGWK"
     gene            complement(1680..2117)
                     /gene="dtd"
                     /locus_tag="AC16CB_01300"
     CDS             complement(1680..2117)
                     /gene="dtd"
                     /locus_tag="AC16CB_01300"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR3341130.1"
                     /translation="MRIVLQRVTMGRVSVNDRTVAQIGSGLVLLVGIGPNDGEEQARY
                     LAEKIANLRIFEDDQGKMNLSVLETGGEALVVSQFTLYADTRKGRRPSFTDAAMPEVA
                     SPLVERFAALLSQQGIPTQTGEFAAHMQVEIHNDGPVTIILER"
     gene            complement(2170..3168)
                     /gene="trpS"
                     /locus_tag="AC16CB_01305"
     CDS             complement(2170..3168)
                     /gene="trpS"
                     /locus_tag="AC16CB_01305"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR3341131.1"
                     /translation="MTETKAKKGRVFSGARPTGRQHLGNYLGAIQNYVALQDEYECVY
                     CIVDLHALTTLDTTENLRQNTYEMALDWLAAGIRPDETIVFVQSQVPEVTELHTILSM
                     VTPLGKLTDLPTFKEKVRMQPHNINYGLVGYPVLQTADITLYKAHLVPVGVDQAPHLE
                     FAREVVRSFNFRYKTDVLIEPQMKATETPKVLGIDGQQKMSKSLDNQIEMAATPDETR
                     ARVMQMVTDPARIRRTDPGNPDVCNVFSLHKVFSSADEVAMINVECRRAGIGCVDCKG
                     IFARNLNAHFSPFRERRAELGKDTNLVWDVLHQGKERAQEIARQTMAEVRQAVGLP"
     gene            complement(3165..3362)
                     /locus_tag="AC16CB_01310"
                     /pseudo
     CDS             complement(3165..3362)
                     /locus_tag="AC16CB_01310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
     gene            3578..4429
                     /gene="amrB"
                     /locus_tag="AC16CB_01315"
     CDS             3578..4429
                     /gene="amrB"
                     /locus_tag="AC16CB_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HIR3341132.1"
                     /translation="MAATLDVRPSPIAGQWYPADPRRLAASVDAYIDQAQLPAIEGQV
                     IAVVTPHAGHRYSGPVAGYAFAALRSLNPDLVAVVSPMHYPEASPILTTAHQAYETPL
                     GSIPVDREALHTLEKELSPSGVNISSVRHDPEHSLEIELPFLQRALTKGFRLLPLMVR
                     DPSPQVAQELGRALASVLAEQQALLVASTDLSHFFSQTVAEVLDQEILRRLAAFDPQG
                     ILQAEEEGKGFACGRGALAAVLWAAKELGGNHVQILHYATSGDVTGDYSQVVGYASAV
                     VTRKEIA"
     gene            4431..5795
                     /locus_tag="AC16CB_01320"
     CDS             4431..5795
                     /locus_tag="AC16CB_01320"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR3341133.1"
                     /translation="MENPYLIDALKLLAVVLLVLANGFFVAAEFSLVSVRRTRVAELV
                     SQGVPAANWVQKAIENPDRVIAATQLGITLASLGLGWIGEPALADLLKPLVELFPATI
                     QSGISHSLSAGIAFAIITFLHVVVGELAPKSIALQSPESTALVIARPTVISEWVFKPA
                     IWLLNGSGNILLKLVGINPASGHELVHSVEELKMLVNQSAEGGIVQAEESEMLHAIFS
                     LGELVVRQVMIPRTEVIGVEADTPLEEIITLATKSTYTKLPVYEDNLDQVLGIVHVKD
                     LLRAMQSPDCDVCTARTLMREPVFVPETISVSALLGQFRENRQHIAIVLDEYGGTAGL
                     VTLEDLLEEIVGEVSDPFDRINPEIQTLADGSALIDGLALIEEVNHHLNLVLQDPNYD
                     TIAGYVLGKLGRIPRVGDAVEGDGVRLKVESMDGLRIARLSLMQTNPPPQPSPLDDGD
                     ERNR"
     gene            5847..8369
                     /gene="priA"
                     /locus_tag="AC16CB_01325"
     CDS             5847..8369
                     /gene="priA"
                     /locus_tag="AC16CB_01325"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication restart helicase PriA"
                     /protein_id="HIR3341134.1"
                     /translation="MPAYVEIAVNVPKVADVFHYHLPEALEGRVDVGHLVEVPFGRQR
                     VQGVVLGFVDKPAIPQTRAVLTTLDPQAVLTRAQLILAQRLAEATLSSLGAYIQLMLP
                     PGLGQQVDTLYTLNPQRSVRPGQSLSALQERLLALLGKRGPLRGRQIDSALPRIDWRP
                     VARSLVRRGLISAESILLSPSVRPKTVRTAQLACPPQVAEARLPELGRASSNALQRRQ
                     AMLRFLMREPGPVEVSWIYAESGGNLADLHKLAELNLVILGESEAWRDPLENIEFQPA
                     QPPKLTTDQQRCWDEIEQALRETATGQSAPPFLLHGVTGSGKTEIYLKAVETTLRMGK
                     QAIVLVPEIALTPQTIRRFVARFPGQVGLVHSRLSAGERYDTWRRARAGDLSVVVGPR
                     SALFTPLPELGMIVVDECHDDSYYQDDPPFYNGREAAVAYARLAGAICLMGSATPDIT
                     SAYQAAQGVWRYLRLPARILAHQEAVRQQADRLGQATRYRPLENLAETADLPPVQVVD
                     MRQELQRGNRSIFSQPLQEALARTLDHKQQAILFLNRRGTATHVFCRDCGYVLKCPRC
                     DTSLTYHSPQEALTCHYCGYQRKLPETCPSCKSRRIRQYGMGTERVEADLQGLFPQAR
                     TLRWDYETTRKKGAHEIILGHFVAGRADILIGTQMLAKGLDLPFVTLVGVVLADVGLN
                     LPDYRAAERTFQVLTQVAGRAGRSPLGGEVLLQTFQPEHYAIQAAAGHDFRSFYRQEL
                     EYRRQLRYPPFNRLVRLEYRHLRAHEAETAAHKLADQLRKWIQDEGRQATELAGPTPC
                     FFSRLAGLYRWQIILRGPDPVSLLRTRNLGEWRVEIDPPSLL"
     gene            8498..9019
                     /locus_tag="AC16CB_01330"
     CDS             8498..9019
                     /locus_tag="AC16CB_01330"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0106130 - purine phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006144 - purine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HIR3341135.1"
                     /translation="MQPTRHEVLTWNDVDRLIDHLLPQFLGEYEAMVMITRGGIIPGG
                     LLAEAMGITHLLTAAVDFPAQIEMEKARLFAWPQFIQFPEEQQIVGRRTLVVDDVWGS
                     GRTITTVRNRVEAAGGFPSTCVLHFNPYRNLFGKARPDYYAAITDAHIIYPWEINRGA
                     DRALLERSPQTPS"
     gene            complement(9132..9611)
                     /gene="sixA"
                     /locus_tag="AC16CB_01335"
     CDS             complement(9132..9611)
                     /gene="sixA"
                     /locus_tag="AC16CB_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HIR3341136.1"
                     /translation="MILYFLRHGLAGSRKDWRGDDFLRPLTKKGMKNMVRQADTFADL
                     DLNLDLIITSPLARAFQTADIVADELKLGEMLVQDERLSPGFGIDDLEQVLNEHAGAE
                     NLMLVGHEPDFSQTISALIGGCRVVCKKGGLARVDIFSTDPLQGELVWLIPPGVMIR"
     gene            complement(9648..>9926)
                     /locus_tag="AC16CB_01340"
     CDS             complement(9648..>9926)
                     /locus_tag="AC16CB_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341137.1"
                     /translation="TVISEIKSMQDHLGDLNDADVAAGILRDFLTRWDTLQAERPVAE
                     RRGPEPILTYLASRYAERQRLMLSFKEAWLNFNRPQLRQELAKAVAVL"
BASE COUNT         2104 a         2969 c         2761 g         2092 t
ORIGIN      
        1 tgcgaggatg gtcccgtgtt tgaagttgat gagatcaaat ggtgaattct ttcatgaata
       61 tcctggcttt ctttgcccac ccagacgatg aaaccgtgct gatcggcggc acactggcgc
      121 tgctagctcg caacggggcg cgcctgcact ttctgtgcgc cacgcgcggt gagggtggcg
      181 aaaccggcga gccctctttg tgtagcgaag aggagctggg taacgagcgc gagaaagaaa
      241 tggtttgtgc ggtacaaaac ctgggtggca gcagcctgac tttcttagat tacgtcgacc
      301 cgcgcgtcgg gccggatgag cagttatacg cttacaccca ggacctgacc ctcctggcgg
      361 gtcaggtagc caccagcatc cgccagttcg aggtggacgc cgtgatcacc cacggatcca
      421 atggcgagta cgggcatccg gcgcatgtgc tcacccacca ggcagctcgc atcgccgtag
      481 aatcttttgg caagggtcac ccattgctct atacagtagc agccaatttt ccggaccacc
      541 cgcggccgcg cctggcaaac cgcgatgacc ccgcccacct ggtcctggat atctcccctg
      601 cgatgagcga gaagacacaa gctgctttat gccatcgcac ccagcatgcc ctattcgtgc
      661 gccgttcgtc ccaagaagcc ggccgccagc tcactgtacc agaggtcatc cagcacctgg
      721 aaagccttca cagggcgctt cctccaacaa atgggggtgt gcaagatgag cttgccaggc
      781 tacttaaacc ctgggaagca catggcactg cgtaaggagc agggactcac actttacgta
      841 tcaagtcaaa gcccatgaaa ttcgacctgg tattcccgac tcccctgatg aacgccgccg
      901 gtagcttggg gttcgccccc gatgtgcggc gtatggcctc cctgggagag cttggggctt
      961 tcgtgaccaa cccagttagc ctaagtcctc gctcaccggc gcaaagccga ggattgatcg
     1021 ccttctcagg ggggttcttg ctgcacagtg gatatcccaa cccaggtctg aaggccgtga
     1081 tccgccgcta tgccttgcgc tgggcgcggg cagacatgcc tgtcatagtg cacctgctgg
     1141 cacagggaga agacgaaatt tcctcgatgg ttagccggtt ggaaaatgtg gatggagtaa
     1201 gtgctgtgga gatcggcctg ccgcctgata ccagtaagga gctagcaatt cgtttgatcc
     1261 aagccggaat tggggagctg ccggtgatcg cccggctgcc gttggagcgc gcacaggaac
     1321 tagcagagct agtttctcaa gcaggcactg cagcagtcag cctaagcccg ccacgcggcg
     1381 ccatccccgc ccctgatggc gggctgctgc gcggccgctt gtacggtccg gcgatttacc
     1441 cccaggcatt gctagttgtt caagcgcttg ccaggtccgg catccccgtg atcggaggcg
     1501 gcggtgttta ccgccacgca gatgccgaag ctatgctttc ggcgggtgca ctggctgttc
     1561 agctcgattc agtgctgtgg cgaggctgga aataacccac acccttctcc aaggatacag
     1621 gaaagcaagg ctggatgcat tgttatccaa ccgactcatt ggggacgcta tccccatctt
     1681 taccgttcca agatgatggt gactggccca tcattatgga tctccacctg catatgggcg
     1741 gcaaactcac cagtttgagt ggggatgccc tgctggctca gcagcgcagc gaagcgctcc
     1801 accagcgggc tggcgacctc cggcatggca gcatccgtaa aagacgggcg gcgacctttg
     1861 cgcgtgtcgg cgtacagcgt aaactgcgaa accaccagcg cctcgccgcc tgtctccagt
     1921 accgagagat tcatttttcc ctgatcatct tcgaaaatac gcaagttagc aatcttctct
     1981 gccagatagc gcgcctgctc ttccccatcg ttcggaccaa tgcccaccag gagcaccaga
     2041 ccggagccaa tctgggcgac ggtgcgatca ttgactgata cgcgacccat agtaacgcgt
     2101 tgcaacacaa tccgcatggg tgtgtcaggg aataaggtct tcccttatcc ccttatctca
     2161 aatcccctat caaggcaatc ccaccgcttg gcgaacttcc gccatcgtct ggcgggcgat
     2221 ctcttgcgcc cgttccttcc cctggtgtag cacgtcccaa accagattgg tgtctttgcc
     2281 gagctcggcg cggcgttcgc ggaaaggcga aaaatgggcg ttcaaattgc gggcgaaaat
     2341 ccccttacaa tccacacagc cgatgcccgc ccgccggcac tccacattaa tcatggcgac
     2401 ttcatcggca gatgaaaaca ccttatgcaa cgagaacacg ttgcacacat ccggattgcc
     2461 cgggtcagta cggcggatgc gggccgggtc ggtgaccatc tgcatcaccc gcgcccgggt
     2521 ttcgtccggt gtggcagcca tctcgatctg gttatccagg ctcttgctca ttttttgctg
     2581 cccatcgatg cccagcacct tgggtgtctc tgtggctttc atctgcggtt cgatgagcac
     2641 atccgttttg tagcggaagt taaacgatcg caccacctcg cgggcgaact cgaggtgcgg
     2701 cgcctggtca acccccaccg gcaccagatg cgctttgtac agggtgatat cagcagtttg
     2761 caacacgggg taaccgacca gaccatagtt gatattatgg ggctgcatgc gcaccttctc
     2821 tttgaacgtc ggcagatcag tcagcttgcc caatggcgta accatggaaa gaatcgtgtg
     2881 cagctcagtc acctcgggca cttgcgattg gacaaataca atggtctcat ccgggcgaat
     2941 acccgctgcc agccagtcga gggccatctc gtaggtattc tggcgcaagt tctccgtggt
     3001 atcaagcgtg gtgagagcgt gcaggtcaac aatacaatac acgcattcat actcgtcctg
     3061 caacgccaca tagttttgga tggctcccag gtaatttcct aagtgctggc ggcctgttgg
     3121 gcgagcccca gaaaacaccc gacctttctt cgccttggtc tcagtcataa attctcccga
     3181 agcagattgc gcaccaaccc cactacttca ccgggttcgg cgtggcgcct ggtttgcatc
     3241 ttagaataaa tcaactcgtt gttcacggtg acctcaaaac gccccccatc agacgggtac
     3301 agggtaagcg attcgatctc aggttcaaac tctttgacaa gttcggctac caggccgacg
     3361 acccggtcag cgtagtgtca tactgcgcag tacgtgatct gaatattgag caacataata
     3421 acacctcgaa tagacactat taaaacacaa cgttgtgcta aaatcaattc agtaaccaaa
     3481 attagattgg tagggcagcg caattcacgc aggcactttc acaattcaaa ccaatccgaa
     3541 tcccgcatcc cctgcctctt atctcctgga ggttgagatg gccgctacgc tcgatgtacg
     3601 cccctctcct attgccggtc aatggtatcc agcggatcca cggcggctag ccgccagcgt
     3661 ggacgcgtat atcgaccagg ctcaattgcc cgctatcgag ggtcaggtga tcgctgtggt
     3721 aaccccgcac gccggtcatc gctattctgg ccctgtagcc ggctacgcgt ttgctgcatt
     3781 acgcagcttg aatccagatc tggtggcggt ggtttcgccc atgcactacc cggaagcctc
     3841 acctattcta accaccgccc atcaagccta tgaaacaccc ctcggttcca tccctgtcga
     3901 tcgcgaggcg ctgcataccc tggaaaagga actaagccca tcgggagtca acatttcatc
     3961 cgttcgccac gacccagagc actcgctgga gatcgaactg cccttcttac agcgggctct
     4021 aacgaaaggt ttccgcctgc tgccattgat ggtgcgcgac cccagccctc aggtagcaca
     4081 ggaattgggg cgggcgctgg ctagtgtgct tgctgagcag caagcgttgc tggtagctag
     4141 cacggaccta tcccatttct tttcgcaaac ggttgccgag gttctagacc aggagatact
     4201 gcgccggcta gcagctttcg atccgcaggg tatactacaa gcggaggagg agggcaaggg
     4261 ctttgcttgc gggcgaggtg cgctggctgc ggtgctctgg gcagcgaaag agttgggggg
     4321 gaaccacgtg caaatcttgc actacgccac ctccggtgat gtgaccgggg attacagcca
     4381 ggtagtgggg tacgcttctg cggtggtcac aagaaaagag attgcatgat atggaaaacc
     4441 cttatttaat tgatgcgctt aaacttttgg cagttgtatt attggtgctg gcaaacggtt
     4501 tcttcgttgc agccgagttt tctctggtca gtgtgcgccg cacgcgcgtc gccgagctgg
     4561 tatcccaggg tgtcccagcg gcaaactggg ttcagaaagc cattgagaat ccggacagag
     4621 tgatcgccgc tacccagctt ggtatcaccc tagccagcct cgggctgggc tggatcgggg
     4681 agccagcttt agctgatttg ttgaaacccc ttgtggagct cttcccagcc accatccaat
     4741 ccggaatctc ccacagcctt tcagccggga tagcgtttgc gatcatcaca tttctccacg
     4801 ttgtagtcgg cgaactagcg cctaaatcca tcgccctgca aagtccagag agcactgccc
     4861 tggtcatcgc cagaccaact gtcatttctg aatgggtttt taagccagcc atctggcttt
     4921 tgaacggcag cggcaatatc ctcttaaagc tggttgggat caacccggcc tccgggcatg
     4981 agctggtaca ctcggttgaa gagctcaaga tgttggtcaa tcagagcgct gaagggggca
     5041 ttgtccaggc agaagagagc gaaatgctgc atgccatctt cagcctgggc gagctggtgg
     5101 tgcgccaggt gatgatcccc cgcacggagg tgatcggcgt ggaggctgac acgcccctgg
     5161 aagagatcat taccctggca accaagtcga cctatacaaa attacccgtg tacgaagata
     5221 acctggatca ggtgctgggg atcgtgcatg tcaaggatct gctgcgggct atgcagtcgc
     5281 cagactgcga tgtatgcacc gccagaacgt tgatgcgcga gccggtcttt gtgcccgaga
     5341 cgatctcagt cagcgctttg ctcggtcagt ttcgagagaa ccggcagcac atcgccatcg
     5401 tattggacga gtacggaggg accgctggct tggtcacgct ggaagatttg ttggaggaga
     5461 tcgtcggcga ggtcagcgat ccctttgacc gcatcaaccc agagattcaa acccttgccg
     5521 atggctctgc cttgattgac ggactggcac tgatcgaaga agtcaaccat catctaaact
     5581 tggttttgca agaccccaac tacgacacga tcgcgggcta tgtcctgggc aagcttgggc
     5641 gcatcccccg agtaggcgat gcggtagaag gagacggtgt acgcctgaaa gtggagagca
     5701 tggacgggtt aagaatcgcc cgtctatccc tgatgcaaac caatccccca ccccagccat
     5761 ctcccctgga tgatggggat gagagaaatc gctagaactc agcttaagca tggttgcttg
     5821 gggataggga cataagaatt cgggttatgc ctgcctacgt agaaatcgcc gtcaatgtac
     5881 ccaaagtagc ggatgtattt cattaccatc tacctgaggc cctggaaggg cgcgtggatg
     5941 tcggccatct ggtcgaagtc cccttcggtc gccagcgcgt tcagggagtg gtgctgggct
     6001 ttgtagacaa gccagccatc ccacaaacac gcgcggtatt aactacattg gacccgcagg
     6061 ccgtgcttac tcgcgcccag ctcatattgg cacagcgcct ggcagaagcg acgctttcct
     6121 cgctgggagc ctatatccag cttatgctcc cgcctggact gggacagcag gtggacaccc
     6181 tgtacaccct caatccccaa cgctctgtga ggccagggca gagcctgagc gctctgcaag
     6241 agcgcctgct ggccttgttg ggaaagcgcg gtcccttgcg cggccgccag attgactcgg
     6301 ctcttccccg tatcgactgg cgacctgttg cccgctccct ggtacggcgc ggcctgatct
     6361 ccgccgaatc gatcttgctc tctccatctg tgcgccccaa gactgtgcgc actgcccagc
     6421 ttgcctgccc gccacaagtt gctgaggcgc gcctgccaga gctggggcgt gcgagttcga
     6481 acgcactgca gcgccggcaa gccatgctgc gcttcttgat gcgcgagcca ggtccagtag
     6541 aggtgagctg gatttacgct gaaagcggag gtaacctggc agacttgcac aagctggctg
     6601 aactgaacct ggtcatcctg ggggaaagcg aagcctggcg cgatccgtta gagaacatag
     6661 aattccaacc agcccagccc ccaaagttga ctacggatca gcaacgctgt tgggacgaga
     6721 tcgagcaggc tttacgagaa acagccactg gccaatccgc ccctcccttc ttattgcacg
     6781 gcgtcaccgg ttccggtaag acagagatct acttaaaggc agtcgaaaca acgctgcgca
     6841 tgggcaaaca ggctatcgtt ctggtgcccg agattgccct cacccctcaa accatccggc
     6901 gcttcgtggc gcgctttcca ggacaggtgg gtctggtgca ctcgcgcctg tctgcaggtg
     6961 agcgctacga cacctggcga agggcgcgcg ccggcgatct gagcgtggtg gttgggccgc
     7021 gcagcgccct gttcacacct ttgccagagc tgggtatgat cgtggtggac gagtgccacg
     7081 acgattctta ctaccaagac gacccgcctt tctataatgg tcgcgaggcg gctgtggctt
     7141 acgcccggct ggcaggcgcg atttgcctga tgggctcagc cacgccagat atcaccagcg
     7201 cctaccaggc agcccagggg gtatggcgtt acctgcgcct gccggcgcgc atcctggccc
     7261 atcaagaggc agtgcgtcaa caggctgatc gattgggcca ggcgacgcgc tatcgcccgc
     7321 tggaaaacct cgccgaaacc gccgatttac ccccggttca ggtggtagac atgcgccagg
     7381 agctgcaacg cggcaaccgc tcgatcttca gccagccact gcaggaagct ttagcgcgca
     7441 ccctggatca caaacagcaa gccatcctct tcttgaaccg gcgtggcacg gctactcatg
     7501 tgttttgccg cgactgcggt tatgtgctca aatgcccgcg ctgcgataca tcgttgacct
     7561 atcacagccc gcaggaggcg ctcacctgtc actactgcgg ctaccagcgc aagctgccag
     7621 aaacctgccc gtcctgcaag agccgacgca tacgccagta cggcatgggt accgagcggg
     7681 tggaggctga tttgcagggc ctcttcccac aagcgcgcac actgcgctgg gattatgaga
     7741 ccacacgcaa aaaaggcgct catgagatca tcctggggca cttcgtcgct ggccgcgccg
     7801 atatcttaat cggcacgcag atgctggcca aagggctgga cttacccttt gtaaccctgg
     7861 tgggtgtggt cctggccgat gtgggtttaa acttgcccga ttaccgcgcc gctgagcgta
     7921 cattccaggt gctcacccag gtggctgggc gcgctgggcg tagcccgctg ggcggggagg
     7981 tgcttttgca aaccttccag ccagagcatt acgccatcca ggctgccgcg ggccatgact
     8041 ttcgcagttt ctatcgccag gagcttgaat accgccgcca gctgcgctat ccgccgttca
     8101 atcgcctggt tcgcttggag taccgtcatc tcagggcaca cgaagcagaa accgccgccc
     8161 ataaactggc tgaccagtta cgtaagtgga tccaggacga gggccgccag gcaaccgagc
     8221 tggctggccc gacgccctgc ttcttctcac gactggcggg gctgtatcgt tggcagatca
     8281 tcctgcgcgg tcccgacccg gtctccctgc tgcgaacccg aaatctgggt gagtggcggg
     8341 tagaaataga tcccccttct ttgctataat aggtgatatt tcccctcagt cccgctttcc
     8401 caagtggtgg gaccaggctc aggacttagg cagcagtaaa aaataacacc gatctgcata
     8461 attcctgaag ttatctttgc acaagatgga gttccctatg caacccacgc gccatgaagt
     8521 cctcacctgg aacgatgttg atcgcttgat cgaccacctg ctgccacaat ttttggggga
     8581 atatgaggcc atggtcatga tcacccgcgg cggcatcatc cctggcggtc tgttagccga
     8641 agcgatgggc atcacccacc ttttgaccgc agcagtagac ttccctgccc aaatagagat
     8701 ggagaaagcc cgacttttcg catggcccca gtttatccaa tttcccgaag agcagcagat
     8761 cgtcgggcga cgcaccttgg tggtggatga tgtgtggggt tccgggcgca ccatcaccac
     8821 ggtcaggaac cgggtggaag ctgccggcgg atttccatct acctgtgtac tgcacttcaa
     8881 cccataccgc aacctgttcg gcaaagcccg accagattac tacgccgcga tcaccgatgc
     8941 acacattatt tacccctggg aaattaaccg cggcgctgac cgagccttgc tagagcggtc
     9001 acctcagacc ccatcctgac catgttttgt caggttcacc catcccgttg gcaagagatg
     9061 aataggatct gcgggtgcac aaatgcccgc agaccccaat gatcatcaat gccttgatta
     9121 tcgagggaaa tctagcgaat catcacccca ggcggtatca gccacaccaa ctcaccctgc
     9181 aacggatccg tgctgaagat atccacccgc gccaggccgc cctttttgca caccacccgg
     9241 cagccgccga tcaacgcgct gatggtctgg ctgaaatctg gctcgtgacc caccaacatg
     9301 agattctctg cgccagcatg ttcattcaac acctgctcca ggtcatcgat accaaaacca
     9361 ggagacaggc gctcatcttg caccagcatc tcgcccaatt tcagctcatc agcgacgatg
     9421 tcagcagtct ggaaggcgcg cgccagcggg ctggtgatga tcaggtccaa attaaggtca
     9481 aggtctgcaa aggtatcagc ctggcggacc atgttcttca tccccttctt ggtcaatgga
     9541 cgcagaaagt catcacccct ccagtctttt cggctgcccg ccagaccgtg gcggaggaag
     9601 taaaggatca tgggttttct ccgaatatgc ttagtacagg ggtgaatcta caaaaccgcc
     9661 accgcttttg ccagctcctg gcgcaactgc gggcgattga aattcaacca ggcttcctta
     9721 aaggatagca tcaagcgctg ccgctccgca tagcgcgaag ccagataggt caatatgggc
     9781 tcagggccgc gccgctctgc aaccggtctc tcagcttgca gggtgtccca acgggtcaga
     9841 aaatcccgca ggatgccagc ggctacgtcg gcgtcgttca gatcgcccag gtgatcctgc
     9901 atactcttga tttcgctgat gacagt
//
LOCUS       DBNGSX010000040         3433 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_694984, whole genome shotgun sequence.
ACCESSION   DBNGSX010000040 DBNGSX010000000
VERSION     DBNGSX010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_694984"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="AC16CB_01345"
     CDS             complement(<1..319)
                     /locus_tag="AC16CB_01345"
                     /inference="COORDINATES: protein motif:HMM:NF014049.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HIR3341138.1"
                     /translation="MTTQLIIGLTLGAIIGWLAWRAGSLSDSGAWAAALTGGLIFGLG
                     GIKWAVLLLAFFVSSSVWSRAFNRRKAHLSNQYAKGSRRDWGQVLANGGLGAVLVVVG
                     YFFP"
     gene            complement(331..1272)
                     /locus_tag="AC16CB_01350"
     CDS             complement(331..1272)
                     /locus_tag="AC16CB_01350"
                     /inference="COORDINATES: protein motif:HMM:NF022338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR3341139.1"
                     /translation="MNTSFEFRPPRGAGIVFNLGGIVLLASGAIWGLWGASQAKNNVV
                     FILYLIPVLVAVLGVPFLTYRVLALRGAFYRLERDGVTLRWGLRSEDIPIDAVLWVRP
                     AAEVGSGLPLPLLRWPGNVVGTRRLSGGGVVEFMAANTRQLVLIATSRLIFAVSPENP
                     AEFISIYHRQTELGSLTPLTPRTVYPTFLLGRVWQRRSARALLLTSLVLNLILVTWVA
                     LVAPVRGEVIMGVQRGGEVVPAVQLMLLPVLSSFFFLVDFFLGLFFYRRGVLLLATST
                     RVGSARNPNMVLAYLLWGSAVITSLMFFSAVAYILLS"
     gene            complement(1273..2505)
                     /locus_tag="AC16CB_01355"
     CDS             complement(1273..2505)
                     /locus_tag="AC16CB_01355"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3341140.1"
                     /translation="MITLLHFADAHIDMANYGRHDPQSGLPLRVMDFLKSLDTIVDTA
                     IEEKVDLVLFAGDAYKDRSPAPTFQREWGKRIMRLSRAGIPILLLVGNHDLSPALGRA
                     HALESFDTLEVPRVRVLEKPEMLTPKDLWGLPLQILALPWISRSGIMAALDLSAADPK
                     QVYEQIEERLTGLIQDWLEAVDPSLPVVLTAHASVQGAIYGGERTVMLGSDLVLAGSL
                     VKDPRLDYVALGHIHKPQNINENAHPPVIYPGSIERVDFGEVKDDKFFVIARVGRGKT
                     EVEWRQLTGIRPFIERYLTLESQEGIAQKLSQALPPAEQIEGAIVRLVLEYPREWESL
                     IDESALREHTAAAFEFQLIKRPQMEARIRLPDGQAVGSLTPLELLNTYWDANHVQADE
                     KEALKNLAQNLIQEREEE"
     gene            complement(2510..>3433)
                     /locus_tag="AC16CB_01360"
     CDS             complement(2510..>3433)
                     /locus_tag="AC16CB_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341141.1"
                     /translation="MALTFWGWEGNRDVVGKAVKPNDKDKNVMPYEMQDFVAEQAPDL
                     DSLVRLGGDIELLKRLVAAGFPVVAEKGYYEYDYTGKLGWLGHYQFVTGYDEAKEVLI
                     VQDTYVKDGKDHEFSYADFIEGWRSFNYVFLVAFPRERAEEVYALLGPYADPTWAARH
                     ALEVAKAETETLTGIDQFFAWFNVGTSHVNLQEYGDATYAFDYAFSTLYPNLPEDKLR
                     PYRVMWYQTWPYWAYYYSGRYQDVIDLANTTLYDTISEPVLEESFYWRGLAREALGDS
                     QGAIDDFRTSVQLNPNFGPGWEQLNRLGVGS"
BASE COUNT          818 a         1062 c          900 g          653 t
ORIGIN      
        1 caggaaaaaa atagcctacc acaaccaata cagcccccaa tccaccattc gccagcacct
       61 gaccccaatc gcgccgcgag cctttggcat attggttaga caaatgagct ttacggcgat
      121 tgaaggctcg cgaccaaacg ctggacgaga caaaaaacgc cagtaacagt accgcccact
      181 tgataccgcc caggccaaag atcaaaccac cggtgagtgc cgctgcccac gctccactgt
      241 ccgagagcga tcctgcgcgc caagccagcc agccaattat ggcgcccaac gttagaccaa
      301 tgattagctg ggtggtcata ctctaattgt tcaggataga agaatgtaag ccactgcgct
      361 aaagaacatg agcgaggtga tcactgcgct cccccatagc aagtatgcca gcaccatatt
      421 ggggttccga gctgacccaa cccttgtgga agtcgccagg agcaatactc cccggcgata
      481 aaagaataac cccaagaaaa aatcgaccag aaagaagaaa ctgctcagca ctggcagcaa
      541 catcaactga actgccggca cgacctcccc accgcgctgc acacccataa tgacctcacc
      601 ccttaccggc gcaaccaggg ctacccaggt tactaatatc aagttgagta cgaggctagt
      661 caacaacagt gcccgtgccg agcgccgctg ccagacacgc cccagcagaa atgtcggata
      721 tacggtgcgg ggggtcagtg gggtgaggga gcccagctcg gtctggcgat ggtagatcga
      781 tatgaattcc gccgggtttt ctggcgagac ggcgaagatg aggcgcgagg tagcgatcaa
      841 caccagttgc cgggtattgg ctgccatgaa ttccacgaca cccccgccac tcaggcggcg
      901 agtacccacg acatttcccg gccagcgcaa caaaggcagt ggcaagccgc tgcccacctc
      961 agccgctgga cgcacccaca acaccgcgtc gatggggata tcctctgagc gcaaccccca
     1021 gcgcagcgtc acaccatcgc gttccaagcg ataaaacgcc ccgcgcaatg ctaagacacg
     1081 gtaggtcaaa aaaggtacac ccaaaaccgc aacaagcacc ggtatcaagt aaagaataaa
     1141 caccacgtta ttcttcgcct ggctcgcccc ccacagcccc caaatggcgc ctgaagccag
     1201 gagcactata ccgccgagat taaatacaat ccctgcgccg cgtggagggc ggaattcgaa
     1261 acttgtattc acctactcct cctcacgctc ctgaatcagg ttttgcgcaa gattcttgag
     1321 ggcctccttc tcatcggcct gtacgtggtt ggcatcccag tatgtattga gcagttccaa
     1381 tggggtcagg ctacccaccg cctgtccatc gggcaggcga atgcgcgcct ccatctgcgg
     1441 gcgcttgatc agctggaatt caaacgcggc agcggtatgc tcgcgcaaag ccgattcgtc
     1501 gatcaggctc tcccactcac gcgggtactc aagcaccagg cgcacgatag ccccttcgat
     1561 ctgttcagcg ggaggcagcg cctggcttaa tttctgagca ataccttctt gagattccag
     1621 ggttaagtag cgttcgataa aggggcggat tccggtcagt tgccgccatt cgacctcggt
     1681 cttgccgcgc cccacacggg caataacgaa gaatttatca tccttcactt cgccgaaatc
     1741 aacccgctcg atagagccgg ggtagatcac gggcgggtga gcgttttcgt taatgttttg
     1801 cggcttgtgg atatgcccca gggcgacgta atccaggcga gggtctttga ccagagagcc
     1861 agcaagcact aagtcgcttc ccaacatcac cgtgcgctca cctccataga tggctccctg
     1921 caccgaagcg tgggcggtca gcacgaccgg caatgaaggg tccacggcct ccagccagtc
     1981 ttggatcaaa ccggtcaggc gctcctcgat ctgctcatac acctgcttgg ggtctgctgc
     2041 gctcaggtct agcgcagcca tgatgcccga acgggagatc cagggcagcg ccaggatttg
     2101 caggggaagc ccccacaaat ccttaggagt cagcatttct ggtttttcta ggactcgcac
     2161 gcgcggaact tccagggtgt cgaagctctc caaggcgtgg gcgcggccca gcgccgggga
     2221 cagatcatgg ttgcccacca gcagcaggat gggaataccc gcccgcgata agcgcatgat
     2281 gcgctttccc cactcgcgct ggaaagtggg cgccgggctg cggtctttgt aggcatcacc
     2341 ggcgaagagc accagatcca ctttctcttc aatcgccgta tccacaatcg tatccagcga
     2401 cttaagaaag tccatcaccc gcaaaggcaa accgctctgc gggtcgtggc ggccatagtt
     2461 agccatatcg atatgtgcat ccgcgaagtg taagagagtg atcatcgtat caggatccca
     2521 cgcccaggcg attgagctgt tcccaacccg ggccaaaatt cgggttcaat tgcaccgagg
     2581 tgcggaaatc gtcgatggcg ccctggctat cgccaagcgc ctcacgcgcc aggccgcgcc
     2641 agtaaaagct ctcttccaat accggctcgc tgatcgtatc atacagggtg gtattcgcca
     2701 ggtcaatcac atcttggtag cgacccgaat agtagtacgc ccaataaggc caggtctggt
     2761 accacatcac acggtatggg cgcaacttat cttctggcag gtttggatac agggtggaga
     2821 aggcgtaatc gaaggcgtaa gtggcgtcgc cgtactcctg caggttgacg tggctggtgc
     2881 ccacattgaa ccaggcgaag aactggtcta tgccggtcag cgtctccgtc tcggctttag
     2941 ccacctcgag ggcatggcgc gcagcccagg tcgggtcagc atatgggccc agcagagcat
     3001 acacctcttc ggcacgctcc ctcggaaagg ccaccaggaa aacatagtta aacgatcgcc
     3061 agccttcgat gaaatcggcg tagctgaact cgtgatcctt gccatccttt acataggtgt
     3121 cctgcacgat cagcacttct tttgcctcat catagccggt gacgaattgg taatgcccca
     3181 accaacccag cttgccggtg taatcgtact cgtaatagcc cttctcggca accaccggga
     3241 agcccgccgc aaccaggcgc ttgagcagct cgatatcccc gccgagacgc accagagaat
     3301 ctagatcagg agcctgctca gccacaaaat cctgcatctc atagggcatg acattcttat
     3361 ctttatcgtt gggcttgaca gctttgccga ctacatctcg gttgccctcc cagccccaga
     3421 aagtcaacgc cat
//
LOCUS       DBNGSX010000041         1982 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1594946, whole genome shotgun sequence.
ACCESSION   DBNGSX010000041 DBNGSX010000000
VERSION     DBNGSX010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1594946"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1256
                     /locus_tag="AC16CB_01365"
     CDS             105..1256
                     /locus_tag="AC16CB_01365"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR3341142.1"
                     /translation="MTEISLDSERQKQAKIYARIQRRLMLVDLALGTVYLIAWLVFGW
                     SAALKGILSQWTANEWYLVAAYGLVFGAGIYVLGLPLSYYSGFILPHRFELSNQTLIG
                     WISDHVKSLLIGGLLGGFLLEVVYAILRATPDLWWLWVAGFLLVFNVLLANLAPVLLF
                     PIFYKFVPLGDEHADLAERLVRLAERAGARVQGVFKFDMSRRTKAANAALAGLGNTRR
                     IILGDTLISEFNPDEIETVLAHELGHHVNKDIPIGIVIESLMTLIGLFLASLGLKWGV
                     VIFGFSSTADIAALPVLALVMGAYGLITMPLGNAYSRWRERRADEYALQATSNGEAYA
                     SALTRLANQNLADADPEPWVEFLLYSHPALSKRIAMANATAPVAIENSE"
     gene            1282..1560
                     /locus_tag="AC16CB_01370"
     CDS             1282..1560
                     /locus_tag="AC16CB_01370"
                     /inference="COORDINATES: protein motif:HMM:NF041918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HIR3341143.1"
                     /translation="MKVNFYATLRQIVGTKIVEVDVPEGITIRELLLEVTRRHPALSR
                     EMFDKSGNLHAHIHVFINGREYSFIEGNLEARVKLSDTISVFPAVGGG"
     gene            1603..1845
                     /locus_tag="AC16CB_01375"
     CDS             1603..1845
                     /locus_tag="AC16CB_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin family protein"
                     /protein_id="HIR3341144.1"
                     /translation="MLMGVDRREFTFEGNKLGELLEAVFAGYDLRDLIFDEEGNIKTW
                     ARVAINGRFSYTIEDMNEAIQEGDLIALLHSYVVAF"
BASE COUNT          452 a          455 c          546 g          529 t
ORIGIN      
        1 cgcattggcg gctcaagcat agatacatag aagaagtctt taatagtttg ttggagtatg
       61 gatgcactac cgattctcat ttggtttcaa gatataatct cgccatgact gaaattagcc
      121 ttgattcgga gcgccaaaag caggcgaaaa tttatgcccg catccaacgc cgcctgatgc
      181 tggtggacct ggccttgggg actgtgtacc tcatcgcctg gctggttttt ggctggtcgg
      241 cagcgcttaa aggtattttg tcccagtgga ctgcaaacga gtggtacttg gtcgctgctt
      301 acggactggt ttttggcgcc ggcatttatg tgcttggctt gcccctttcg tattacagtg
      361 gctttatcct gccacaccgt tttgaacttt ccaaccagac ccttatcggt tggattagcg
      421 atcatgtgaa atccttgtta atcggtgggt tgttgggcgg ttttcttttg gaagttgtct
      481 acgccatatt gagggctaca cccgatctat ggtggttgtg ggtagccgga ttcctgctcg
      541 tgtttaatgt tctactggct aacctggcac cggtgctgct attcccgatc ttttataaat
      601 ttgtccccct gggagacgag catgctgatc tggctgagcg actggttcgc ctggctgagc
      661 gagccggtgc gcgcgtgcag ggtgtgttca agtttgatat gagccgtagg acgaaagccg
      721 ccaatgccgc ccttgcaggg ttgggtaata cacggcggat catcctgggc gatacattga
      781 tcagtgagtt caacccagat gagatcgaga ctgtgctggc ccatgagcta ggccaccatg
      841 tcaataaaga tatcccgata ggcatcgtca tcgaaagttt aatgacactt atcggcttgt
      901 tcctggcttc attgggactg aagtgggggg ttgtgatatt tggattcagt agcacggcgg
      961 acatcgccgc cttgccggtc ttggcgctgg tgatgggtgc ctatgggctg atcaccatgc
     1021 ctctaggcaa tgcctattcg cgctggcggg agcggcgggc agatgaatat gctctacaag
     1081 ctacttcaaa cggcgaagcc tatgcatcgg cgttaacccg cctagcgaac cagaacctgg
     1141 cggatgccga tccagaaccc tgggtagagt tcctgctgta ctctcaccct gccttgagca
     1201 agcgcatcgc gatggctaat gcaactgccc cggttgctat tgaaaatagt gaatgataat
     1261 gtatactaac ctcagaagga tatgaaagta aatttctatg caactctgcg ccaaatagtg
     1321 ggcaccaaga tcgtggaggt cgatgtgccc gagggaatta cgatccggga gctgctccta
     1381 gaagttactc gccgacatcc agccttgtct cgagagatgt tcgataagag cggtaacttg
     1441 cacgcgcata tccatgtatt catcaacggt cgggaatatt cgttcattga aggtaatctg
     1501 gaggctcgag tgaaactgtc ggataccatc agtgtgttcc ctgcggtggg aggaggctaa
     1561 ccgtagactc gagttgtact gaagttcact ggggatgtgc gcatgctgat gggtgtcgac
     1621 aggagagaat ttaccttcga gggaaataag cttggcgagt tattggaggc agtctttgct
     1681 ggatatgacc tgcgcgattt aatctttgat gaagaaggca acatcaaaac ctgggctaga
     1741 gtagctataa atggtcgctt ttcttataca attgaggata tgaacgaagc tatccaggag
     1801 ggcgatttga tagccctcct tcactcttat gtggttgcgt tctgaaaaaa tttatacatg
     1861 attaaccccc gaatttaaac tctctgggat taatcacata catcaactcc taaccctgcc
     1921 ctccccctgt ctagccgaac ggcaggggga gggaaaatga gccaaaatag ggtcttgcgg
     1981 gc
//
LOCUS       DBNGSX010000042         5751 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_487567, whole genome shotgun sequence.
ACCESSION   DBNGSX010000042 DBNGSX010000000
VERSION     DBNGSX010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5751)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_487567"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="AC16CB_01380"
     CDS             complement(<1..787)
                     /locus_tag="AC16CB_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012336.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR3341145.1"
                     /translation="MTDVVIAGIGQTEVSEHWGVSLRELALRAIEAAQEDAGGLQPQA
                     LFVGNMLAPTLSRQAHLGSLVADFAGLTGIEAMTIEAAGASGGAALRTGYLAIASGQV
                     DVAFVVGVEKFSDQVGAPVEEALASSTDSDFESIQGMTPTAQAALLMQRYLHEFGAPR
                     QAFAGFALTAHANGASNPYAMFRKAISAEIYNRAGFVSEPLNLFDVAPNADGAAALVL
                     TRPEMLPPGFPHPLVKIAGSSLVTDTLALHDRPDPLTFQAARVS"
     gene            complement(795..1871)
                     /locus_tag="AC16CB_01385"
     CDS             complement(795..1871)
                     /locus_tag="AC16CB_01385"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HIR3341146.1"
                     /translation="MDHTSLDHLLLKPNRAVGIVGYGAYVPRFRLPASEVSRVWTDGQ
                     AGLPIKEKAVAGIDEDVATMSIEAARNALKRARIEPTHIRAVWVGSESHPYAVKPTST
                     IVAEAIGAVPNVQAADWQFACKAGTEAMVAALGFIGSGMADYAMAIGMDIAQGRPGDA
                     LEYTAGAGGAAYLLGRAEDSLAEIESAYSFVTDTPDFWRRANQKYPEHGQRFTGEPAY
                     FKHITEAAQTLMDATHTTPADYQYAVFHQPNTKFPERVAAQLGFKKEQIATGLLVPVI
                     GNTYAGAALIGLTAVLDIAKPGDRILLVSFGSGAGSDAFALRVTEAIEERRDLAPHTT
                     EYIARRTQIDYATYTRLRGKLVMK"
     gene            complement(1875..3164)
                     /locus_tag="AC16CB_01390"
     CDS             complement(1875..3164)
                     /locus_tag="AC16CB_01390"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HIR3341147.1"
                     /translation="MSEKMSSSRLPGFYNLGIHERRAMLSKFVSAQDLAAMSGEAGLT
                     VEQADHMIENVIGLHGIPLGIALNFVVNGRDVLVPMAIEEPSVVAGASFMARLARAGG
                     GFIAHTTPPEMIGQMQVLEVAEPRSARMALLEQKESLLAEAAEIDPVLKDLGGGPRDI
                     EVRLIEDSPIGAFLVIHLIYDVRDAMGANAVNTACERLAPAVEAITGGRVHLRILSNL
                     ADRRLGRAQCTIPVEQLAFGEYTAEEVRDGVIAAWAFAAADPYRAATHNKGIMNGVDA
                     VVIATGNDWRAIEAGAHAYAARGGRYTSLSTWGVGDQGNLVGTLEMPMAVGVVGGATK
                     VHPTARAALALMGVTSASELAEIIVSVGLAQNLAALRALATEGIQRGHMSLHARQVAI
                     AAGAQGEMIERLAQRLVAEKTVRVDRAEAILRQWQEE"
     gene            3341..5014
                     /locus_tag="AC16CB_01395"
     CDS             3341..5014
                     /locus_tag="AC16CB_01395"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HIR3341148.1"
                     /translation="MALLTREQLEERLAALHRASLELVRELSLDTVLERIVHLAREQA
                     DASYAALGVLDEEGNLVRFIPVGMSAEAVRQMENPPVGMGLIGAMSREYRTIRVANIQ
                     DDPRSEGFPAHHPRMKSFLGVPVLRGDKLLGQIYLTDKESFPEFTEADERVIETLASY
                     AAIAISNARLYEDLLDRDVALDQRNQDLSLINDIATALAGSLDTDEIINQTLIRVMQY
                     LDVDAGEIFLREESEKEMRLALHRGEFAEAFTTRDHFRIGEGFIGLVAQSGQPLITCD
                     LTNDLRFLRPAVVTAGFKCVACIPLTAHGTVVGVLSVASREDRPFDDREVRILTAIGA
                     WAGVAVENARLHRQARRLAVLEERERIGMDLHDGIIQSIYAVGLALEYARVAIDEDPR
                     QSQYKIDQAIEGLNKTIRDIRAYILDLRPRHFVGLNLKEGLQRLVDEYRTNTLSDAVL
                     TAQQDGLPNLPSSHSTALFHICQEALANAAKHAQARRLEVHMWATKDRMLLEVSDNGQ
                     GFDLRKMSVTLGHGLSNMHVRARKVGGDVEITSAPGEGTTILAWVPRRE"
     gene            5014..>5751
                     /locus_tag="AC16CB_01400"
     CDS             5014..>5751
                     /locus_tag="AC16CB_01400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012797.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR3341149.1"
                     /translation="MDLISSRTNPKIKQIRALRGRQGRQQSDLFLVEGIFHVGEAVAA
                     TQAGKLGLDSVFYAPELLKSEFALELVQGLRRQEGAARIPCYELTRQVFESIAEKDNP
                     QGILAIARPVHYELKALNTSNFSWGVALVAPQDPGNLGTILRTIDAVGASGLLLLDSN
                     LDPYHPSAVRASMGALFWYPLVQASFAEFASWVTERGYTVYGTSAHASTDYRQLASAG
                     PPSRSSRPDQTRAQIYTKPMILLLGSER"
BASE COUNT         1177 a         1638 c         1671 g         1265 t
ORIGIN      
        1 cggatactcg ggctgcttgg aaggtcaatg ggtctggccg gtcgtggagc gccagcgtat
       61 ccgtcaccag gctcgatcca gctatcttta ccagcgggtg agggaagcca ggtggtagca
      121 tttcaggtcg ggttaacacc agggcggctg cgccatcagc attgggggct acatcgaata
      181 agttgagcgg ctcactgaca aagccagctc tgttataaat ctcggcgctg atcgctttgc
      241 ggaacatggc gtatggattg cttgctccat tggcatgggc ggtcagggcg aagccagcga
      301 aggcttggcg gggggcgccg aattcatgca ggtagcgttg catcagcagg gcagcctggg
      361 ctgtgggcgt catcccttgt attgattcga aatcactatc ggttgacgag gccagcgcct
      421 cctcgacggg cgcgcccacc tggtcgctga atttctccac acccaccacg aaggccacat
      481 ctacctgccc agaagcgatt gccaggtagc cagtgcgcaa ggctgcgcct cccgaagcgc
      541 cagccgcttc gatggtcatc gcttcgatgc cggtcaatcc ggcaaaatcg gctaccagac
      601 tgcccaggtg cgcctgccgg gagagggtgg gcgccagcat gttgcctacg aatagcgcct
      661 gtggctgcag gcctccggca tcttcctgcg ccgcttcaat ggctcttaac gccaactcgc
      721 gcagggaaac accccagtgc tcactgactt ctgtttgtcc aatacctgca atgacgacgt
      781 ctgtcatttt tcctctattt catcaccagc tttcctcgta atcgcgtata agtggcgtaa
      841 tctatttgtg tgcggcgggc gatgtactcg gtagtgtgtg gtgcaagatc gcggcgctct
      901 tcgatagcct cggtcacacg cagggcaaaa gcatcggagc cggctcctga gccaaaagaa
      961 accagcagga tccggtcgcc gggcttggcg atatccaaaa ccgcggtaag accgattaaa
     1021 gccgccccgg cgtaggtgtt gccgatcaca ggcaccaaaa gtccggttgc gatttgttcc
     1081 tttttgaatc ccagttgcgc agccactcgc tcgggaaact tagtgttagg ctggtggaat
     1141 acggcatact ggtaatcggc tggggtggtg tgggtggcgt ccattagagt ctgggcggcc
     1201 tcggtgatgt gcttgaaata ggctggctcg ccggtgaagc gctggccgtg ctcgggatat
     1261 ttttggttgg cgcgtcgcca gaaatcgggc gtatcggtga caaaggagta ggcgctctcg
     1321 atttctgcca gcgagtcttc tgcccgtccg agcaggtatg ctgcaccacc cgctccggcg
     1381 gtatattcta gggcgtcgcc agggcgcccc tgggcgatat ccatgccaat tgccatggca
     1441 taatctgcca tgcccgatcc aatgaagccc agggctgcca ccatcgcctc ggttccggct
     1501 ttgcaggcga actgccagtc ggcagcctgc acattaggca ccgctccgat agcctctgcc
     1561 acgatggtcg aggtgggctt gacggcgtag gggtgcgatt cggaaccgac ccatacggcg
     1621 cggatgtggg tgggttcgat cctggcgcgt ttcaaggcgt tgcgagctgc ttcgatagac
     1681 atggttgcca catcttcgtc aattcccgca acagcttttt ctttgatggg cagcccggct
     1741 tgaccgtcgg tccacacgcg agacacttcg ctcgccggca agcgaaagcg cggtacgtat
     1801 gcaccataac cgacaatccc taccgcacgg ttgggtttca gtaagagatg atctaatgaa
     1861 gtatgatcca taaattattc ctcctgccac tggcgcaaaa tcgcctcagc gcgatccacg
     1921 cgcacagttt tctcggctac caggcgctgt gctaatcgct cgatcatttc tccctgtgct
     1981 ccagccgcga tagctacctg gcgggcatgc aggctcatat gaccgcgctg gataccctcg
     2041 gtggccaggg cgcgcaaggc tgccaggttt tgagctaaac ctaccgagac gataatttct
     2101 gccagctcag atgcgctggt cacgcccatc aaagccagcg cggcgcgggc agttgggtgc
     2161 actttggtcg ccccaccgac cacgccgact gccataggca tctccagcgt gccgaccagg
     2221 ttaccctggt cacccacccc ccatgtactc agcgaggtat agcgtccacc gcgggccgcg
     2281 taggcgtgtg ccccagcttc gatggctcgc cagtcgttac cagtcgcaat gaccactgcg
     2341 tccacaccat tcataatgcc cttattgtgg gtagcggcgc ggtaggggtc ggcagcggcg
     2401 aaggcccaag cggcgatgac gccatcgcgc acttcttcgg ctgtgtattc gccaaatgcc
     2461 agctgttcca cagggatggt gcattgggcg cgcccaagac gccggtcggc caggttggat
     2521 aggatgcgca ggtgcaccct gccgccggtg atcgcctcaa ccgctggagc caggcgctcg
     2581 caagctgtgt tcactgcatt ggcgcccatg gcatcgcgca catcatatat caggtggatg
     2641 accaggaatg ccccaattgg cgaatcctca atcaggcgca cctctatatc gcgcggacca
     2701 ccgcccaggt cctttagcac tggatcaatt tccgctgctt cggccaggag gctttctttc
     2761 tgctctagca aggccattcg tgcggaacgg ggttcagcta cctccagaac ttgcatctga
     2821 ccgatcatct ccggtggggt ggtgtgggcg atgaatcccc cgccggcgcg ggccagccta
     2881 gccataaaag atgccccggc tactaccgat ggctcttcga ttgccatcgg caccagcaca
     2941 tcacgcccat tgaccacgaa attgagtgca attcccagag gtatgccgtg caagccaatc
     3001 acattctcaa tcatgtggtc agcctgctct acggtcaagc cggcctcgcc gctcattgcc
     3061 gccaggtctt gcgctgagac aaatttcgac agcatagccc ggcgctcatg aatgccaagg
     3121 ttatagaatc caggaagacg tgaactgctc attttttcgc tcatggtagg tttcggtctc
     3181 aagcctcgcc atttatacgg gtgacccgct ggcaaaaact atcaggttgc ctaataaccc
     3241 tgcaagggtc agtagattac agctggaatt gtgaacttca gcactttccc tcaaaagcgg
     3301 aggaaatgga gtaaaataca tatgtggtga ggtaccccaa atggcgttgt taactcgcga
     3361 gcaactagag gagcgattag ctgctcttca tcgtgccagc cttgaactgg tgcgcgaact
     3421 ttctcttgac acggttctgg agcgcattgt tcatctggcc agagagcagg ccgatgcttc
     3481 ttacgctgct ctgggtgtgc tggatgaaga gggtaatctt gtccgcttca tccctgtagg
     3541 catgtctgcc gaggcggttc gccagatgga gaaccctccg gtgggtatgg gtttaatcgg
     3601 cgccatgagt cgggagtatc gcaccatccg cgttgccaat atccaggatg accctcgtag
     3661 cgagggtttc ccagcacatc atccgcgtat gaagtccttt ctgggtgtac cagttctaag
     3721 aggggataaa ctgctagggc agatctatct aacagataaa gaatctttcc cggagtttac
     3781 cgaagctgac gaacgagtaa ttgagacgtt ggcttcttat gcagctattg ctatcagcaa
     3841 cgcgcgttta tatgaggatt tgctcgaccg cgatgtggca ttggatcagc gtaaccaaga
     3901 tctatcgctg atcaatgata tcgccaccgc gttggctggc tcgttggata cggatgagat
     3961 tatcaaccaa accttgattc gggtgatgca atatttggat gtcgatgctg gggaaatatt
     4021 tctgcgcgaa gagagtgaga aagagatgcg cctggcattg caccggggcg agtttgctga
     4081 ggcgtttacg acccgcgacc acttccgtat cggtgaaggc ttcattgggt tggttgccca
     4141 atccggacag ccactgatca cctgcgattt gacaaacgat ttgcgcttcc tgcgtccggc
     4201 ggttgtgaca gcggggttca aatgtgtggc ttgcataccg ctcacagcac atggtacggt
     4261 ggtcggcgtg ctgagcgtgg ctagccgcga ggatagacct tttgacgatc gagaagtgag
     4321 aatactgacc gccatcggcg cctgggcagg ggttgcggtt gagaatgccc gtttgcatcg
     4381 tcaggcacgc cgcttagctg tcctggaaga gcgtgagcgc atcggcatgg atcttcacga
     4441 tggcattata cagtccatct acgccgttgg cctggctctg gagtacgccc gcgtggcaat
     4501 cgatgaagac cccagacaat cgcagtataa gatcgatcag gctattgagg ggttgaacaa
     4561 aacgatccgg gatatccgtg cctacatcct tgatctgcgc ccgcgccatt ttgttggcct
     4621 gaatcttaaa gagggtttgc aacgcctggt cgacgaatac cgcaccaaca cactctccga
     4681 tgcggtcctc actgcacaac aagacggctt gcccaacctg ccctcttcgc actccacagc
     4741 ccttttccat atctgtcagg aggctctggc caatgccgct aagcatgctc aagcgcgacg
     4801 cctggaggta cacatgtggg cgaccaaaga ccgcatgctc ctggaggtct cggataatgg
     4861 ccaggggttc gatttgcgca agatgagcgt cacgctgggt cacgggcttt ctaacatgca
     4921 tgtccgcgct cgcaaagtgg ggggagacgt agagatcacc tcagctccgg gagagggtac
     4981 caccatctta gcctgggtcc cccgccggga ataatggacc tgatttccag tcgcaccaac
     5041 cccaaaatta aacaaattcg cgccctgaga gggcgccagg gacgccagca gagcgacttg
     5101 tttttagttg agggcatttt ccatgtcggt gaagcggtcg ccgcgacaca ggctggaaag
     5161 ctggggcttg attcagtctt ctacgcccca gagctgctca aaagtgaatt tgccctggag
     5221 cttgtgcagg ggctgcgccg ccaagagggg gcggctagaa ttccgtgtta cgagttgaca
     5281 cgccaggtgt ttgaatccat tgcagaaaag gacaaccccc agggcattct agcgattgct
     5341 cgcccagtac attacgagct aaaggcgctg aatacttcaa atttttcctg gggggtggcg
     5401 ctggttgccc cgcaagaccc aggcaacctg gggacaatat tgcgcacgat cgatgcggtg
     5461 ggtgccagcg ggctgctcct gctggatagc aacctggacc cataccatcc cagtgcggtg
     5521 cgcgccagca tgggggcgtt gttctggtac ccgctggttc aggcctcttt cgcagagttt
     5581 gcgtcttggg tcacggagcg aggctacaca gtgtacggca cttcagccca cgcctcgaca
     5641 gactaccgtc agcttgcctc ggctggtccg cccagccgat cctcgagacc agatcaaacg
     5701 agggcgcaga tctatacgaa gcccatgatc cttctgcttg gcagcgaacg c
//
LOCUS       DBNGSX010000043         2791 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1734091, whole genome shotgun sequence.
ACCESSION   DBNGSX010000043 DBNGSX010000000
VERSION     DBNGSX010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1734091"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="AC16CB_01405"
     CDS             <1..552
                     /locus_tag="AC16CB_01405"
                     /inference="COORDINATES: protein motif:HMM:NF036531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HIR3341150.1"
                     /translation="ISKEGPGRLYYRLGLRYAPTDLKLDPVDMGFVVTRSYEAVDDPK
                     DVSRDDDGVWHIRAGARVRVRLNMVADNRRYHVALVDPLPAGLEIVNPSLEVSESIPQ
                     DPNSSNYRYGWWWWGSWYEHQNLRDERAEAFTSLLWDGVYQYTYIARATTPGVVVVPP
                     SKAEEMYSPEVFGRSSSDVVVVK"
     gene            715..1452
                     /locus_tag="AC16CB_01410"
     CDS             715..1452
                     /locus_tag="AC16CB_01410"
                     /inference="COORDINATES: protein motif:HMM:NF015827.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hemolysin"
                     /protein_id="HIR3341151.1"
                     /translation="MFKYHFIVLWVFPTLALLLTACNPQPSIHPTENPPEISEVNIAN
                     PASAYCEEQGGKVEIREGEAGQYGICIFPDGSGCDEWALFHGVCRYGAKDTTSLQGIT
                     ANYVNLNYGFTLNPSEAWTIEDHIDSLLLKRPGYCLFIGFKPVGQALPLFRTGMPAGD
                     FVDGGEFQWLGSPARKNLLVYEGQTLLVNFCQQDQEDCLIAGDLSFVIWLEPCTENAE
                     ASPASNILADEIIEAQKVVESLTLLGK"
     gene            1779..2648
                     /locus_tag="AC16CB_01415"
     CDS             1779..2648
                     /locus_tag="AC16CB_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341152.1"
                     /translation="MNALRRILGALVMIAGILGIILSLAGVVSVWVVKPNVTNYVEIT
                     IQTIETSVITSQKALQVTGQALGATVVSVDALSTMLSTTAVSVEETQPVLDQLNVMMG
                     ETMPSSMESASDSLKTAQQAAVVLESAIKSLENFRAAMSATPLLSSLVEQPKQTYNPE
                     VPLADSLGELATTLESLPATFTEMSANLDKADDNLITIQSNLVTMSDSVGQISKSLSE
                     YQAMITQSQSSMENLISILTNINNNLTSILNGVAMAFSLFFLWLLVAQVVILTQGWEL
                     YQGTASSMESSES"
BASE COUNT          702 a          686 c          744 g          659 t
ORIGIN      
        1 atcagcaaag aaggcccggg acggctctac tatcgcctgg gattgcgcta tgcccccacc
       61 gacctgaagc tggacccggt ggacatgggt ttcgtcgtca cgcgttccta cgaagccgtg
      121 gatgacccca aggatgtgag ccgagatgac gatggcgtat ggcacatccg cgctggggcg
      181 cgcgtgcggg ttcgcctgaa catggtagca gataaccgcc gctatcatgt agccctggtt
      241 gatccgctcc cagccggtct ggagattgtt aacccgtccc tagaggtgtc tgagagcatc
      301 ccccaggatc ccaacagcag taactatcgt tatggctggt ggtggtgggg aagctggtac
      361 gagcaccaga acctgcgcga tgagcgcgcc gaggctttta cctcgctgtt gtgggacggg
      421 gtctatcaat atacctacat cgcccgcgct acaacgcccg gcgtggttgt ggtgccgccg
      481 tccaaggcag aagagatgta ctcgccggag gtctttggtc gcagcagcag cgatgtagtg
      541 gttgtaaagt agggagacaa caccgtaata gataaaaacg ggtgccatgc atccgttttt
      601 tctttctcca gacagagact gaggaacctc cattcaccaa agagcgtcta atacacaacc
      661 tgcgttgtgc atacttcgag caaagggata attcagtcgt tttaggaggg acctatgttc
      721 aagtatcact ttatagtttt gtgggtgttt ccaacgctgg cactcctatt aaccgcatgt
      781 aatccgcaac caagcattca cccaaccgag aatccacctg agatatccga agtcaatatc
      841 gctaatcctg cttctgctta ctgtgaagaa caaggcggta aagtagagat acgcgaaggc
      901 gaagcgggcc aatatggtat ctgcatcttc ccagatggca gcggctgcga tgagtgggcg
      961 ctctttcacg gcgtttgcag gtatggcgct aaagacacca cctctctgca agggatcacc
     1021 gcgaactatg tcaacctcaa ttacggtttt accttaaacc catctgaggc atggacaatt
     1081 gaagatcaca tcgacagcct gctgctaaaa cgccctggat attgcctgtt cataggattc
     1141 aagcctgtcg gacaagcctt gcctctgttc cgtactggca tgccggctgg ggattttgtg
     1201 gatggaggcg aattccaatg gctgggaagc ccagccagga aaaacctgct tgtttatgaa
     1261 ggccaaacgt tgctggtgaa tttctgccag caagaccagg aagactgtct catagcaggt
     1321 gatctaagct tcgttatctg gcttgagccg tgtactgaaa atgcagaggc atccccagca
     1381 tcgaacatcc tggcagatga gatcatagaa gcgcaaaagg tggtcgaatc gcttacgctg
     1441 ttaggcaaat gaactctttc gtattatcac aatcattatg aattctgttg gcaggtccgt
     1501 gccgcggatg cctgccagct ccgcaaggtt ggcgaaggtc tccggggagg gattctcgag
     1561 ggcgcgggcg agttctcctt agaagaagcc ggcgtccaag gatgagactt cttgacggtt
     1621 tctacgcatt cgccaaggtc acgcagcaag gcattatctg agatgtgcag gttgcacggc
     1681 gagagagggg tagtctcaaa caaatgttat cctgttgttc ctgtcgcgat ataattccga
     1741 agcaggctga ttttacaaag gataccagag gaggagatat gaatgcccta cgtcgtattc
     1801 tcggtgcgtt ggttatgatt gcgggaatcc taggcataat acttagcttg gctggagtgg
     1861 tttcggtttg ggtcgtgaag cccaatgtga ctaattatgt tgagatcacc atccaaacaa
     1921 tcgaaacgag tgtcataacc agccagaaag ctctgcaggt cacggggcaa gcattgggag
     1981 ctaccgtggt tagcgtggat gcgctctcca ccatgttgag cactactgca gtttcagtgg
     2041 aagaaaccca acccgtgtta gaccagctca atgtcatgat gggcgaaacg atgccctcct
     2101 ctatggaatc agccagcgac tcgcttaaga ctgcccagca ggcagctgtg gtgctggaaa
     2161 gcgccatcaa gtcgctggaa aacttccgag cagcgatgag tgccactcct ttactaagct
     2221 ccttggtgga gcagcccaag caaacctata atccggaggt accacttgcc gattcattgg
     2281 gtgagttggc taccacccta gaaagtctac cggcgacatt cactgagatg tcggctaacc
     2341 tagataaagc tgatgataat ttgatcacca tccaaagcaa cttggtcacg atgtcggaca
     2401 gtgttgggca gatctcgaaa agcctgagcg agtaccaggc tatgatcact caatcgcaat
     2461 cttccatgga gaatctaatc tcaattctca ccaacattaa taacaatctg acaagcatac
     2521 tgaatggggt ggcgatggca tttagcctgt tcttcttgtg gctgctggtt gcccaggtag
     2581 tcatcctcac ccagggatgg gaattgtacc aaggcacggc aagcagcatg gagagcagtg
     2641 aatcttgagc aatgcaatat aaaaccttta ccgagttgca tattatcaga catatcgatt
     2701 tatttttata ggcactccag ggcatataca cttgagacca tccttggtca actatgagac
     2761 gattcaagct cgggattgca caataaaata t
//
LOCUS       DBNGSX010000044         2820 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_556719, whole genome shotgun sequence.
ACCESSION   DBNGSX010000044 DBNGSX010000000
VERSION     DBNGSX010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_556719"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1342)
                     /locus_tag="AC16CB_01420"
     CDS             complement(<1..1342)
                     /locus_tag="AC16CB_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341153.1"
                     /translation="MQKSPDSVIPTVVQTRFRTPQLPLRSIANFIVLTSLFAIAYSQS
                     PLYTSNQNQYFLHGYASAGYGNLSRDWLAKTLDPTPIFSKLIELTALYLQQEITYYLY
                     YALLMGIYLFSLSGMASTLFDLRNSRSKSMAFTVLFIAIHSAGLRFALSRWLGVNWTY
                     VLEDGVADQRMLGPVFQPSAFGVFLALSLYLFLQRKPYRSLLAASIAATFHPTYLLSA
                     AMLAMAYALVDWIEGKRLSIPIKYGLAALLFVSPILAYVFTAFGNTSTEIASRAQEIL
                     AAIRIPHHVMINQWFDATVIAKLALVAGAIYLALRRHSFQATDTPRSPAWRLGLILLI
                     VSLECSLLTLLQVITQSQALALLFPWRLSILIVPVSTTLILAYFVERIMDTQAAQPAH
                     RKRLVRLLSTFILFMVVLVGGIRFWLDLGRKTAEPEQTLYSYVYTHNSRNDIYLI"
     gene            1435..1800
                     /locus_tag="AC16CB_01425"
     CDS             1435..1800
                     /locus_tag="AC16CB_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341154.1"
                     /translation="MKDHIRIIAWLYIILGVLGIFGALIAFAVIAGGGMISGDETAIY
                     VTGIVALVIAVIVVLVSVPGIIAGLGLLKFKPWARILAIILALLNLPGFPIGTALGIY
                     TLWAMLDSESSQLFNVIEG"
     gene            complement(1863..2270)
                     /locus_tag="AC16CB_01430"
     CDS             complement(1863..2270)
                     /locus_tag="AC16CB_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3341155.1"
                     /translation="MNTVNLPAEYKSLEKISKFVIQAAREAGLSDQDIYEVELAVDEA
                     CSNIIDHAYGGEGIGDMRCSVEVGKGTLKIILNDHGRPFDLALVPEPVVNIPLQKLKR
                     RGVGVYLMRKMVDELHYESSKEAGNVLVLIKRK"
     gene            complement(2277..2612)
                     /locus_tag="AC16CB_01435"
     CDS             complement(2277..2612)
                     /locus_tag="AC16CB_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3341156.1"
                     /translation="MEISTTEYKRCDVVEAKGRVDSSTAPQLEEALKSITDSGRFKIV
                     LDMSEVTFMSSKGWWVLIDTQKTCKRYKRGELVLVNVRDEIQDSLNLVGMGSYFNIFT
                     DLTSAVGSF"
BASE COUNT          719 a          649 c          738 g          714 t
ORIGIN      
        1 gtatcaggta gatgtcgttc cgagaattat gcgtatagac ataagagtac agcgtttgtt
       61 ccggctctgc ggttttgcgt cccagatcaa gccaaaagcg aattccccca accagtacca
      121 ccataaagag aatgaatgtg ctcaataagc ggacgaggcg cttacgatgg gcgggttgtg
      181 ctgcctgggt gtccatgatc cgttccacaa aataggccag gatcaacgtg gtggaaacgg
      241 gtacgatcag gatggataac cgccaaggga agagcagggc taaagcttgg ctttgagtta
      301 tgacttgtaa gagggtgagc aggctgcatt ccagcgaaac gatgagcaga atgaggccta
      361 accgccaggc tgggcttcga ggggtatccg tggcttgaaa gctatgtcga cggagtgcca
      421 ggtagatcgc accggctacc agtgccagtt ttgcgatgac agtagcatca aaccactgat
      481 tgatcatcac atggtggggg atacggatag ctgccaggat ttcctgcgca cgagaggcga
      541 tttctgtcga ggtgttgccg aacgcagtaa atacgtaagc caggatgggt gacacgaata
      601 gtaaagcagc caggccatat ttgatgggga tcgagagtct tttaccttcg atccagtcaa
      661 ccagtgcata agccatcgcc agcattgcgg cgctgagcag gtaggtgggg tgaaatgtgg
      721 ccgcgataga tgcggccagc aggctccggt agggttttcg ttgtaagaag agatagaggg
      781 agagcgccag gaacaccccg aaggcgctag gctggaagac tggcccgagc atgcgctggt
      841 cggcaacccc atcttcgagc acgtaggtcc aattcacccc cagccaccga gacagggcaa
      901 agcgcagccc tgccgagtgg atcgcgataa acaatacggt aaatgccatg gatttcgagc
      961 gagagttacg aagatcgaac agggtgctcg ccatgcctga caggctgaac aagtagatgc
     1021 ccattaatag agcatagtat agataatatg taatctcttg ctgcaggtat agggcagtca
     1081 gctcgatcag cttgctaaag ataggggtgg gatccagagt tttggctagc cagtctcggg
     1141 acagattccc ataacctgcc gaggcgtatc cgtgcaagaa gtattggttt tggttagatg
     1201 tgtaaagagg ggattgggag tatgcaatcg caaacaggct ggtcagcacg ataaaatttg
     1261 cgatggagcg aagaggcaat tgtggagtac gaaagcgcgt ttgcacaact gttggtataa
     1321 ccgaatcggg ggatttctgc aaggcttgtg tccatccaag gtatgcaaca aagtcgcata
     1381 gattacgtaa ataatatcag gaacacaaaa ctgggtcaca tatggaggat caacatgaaa
     1441 gaccacatca gaattatcgc ctggctatat attattttag gggtgctggg aatatttggg
     1501 gcgctgattg cctttgccgt catagcaggg gggggaatga tctctggcga tgaaacagcc
     1561 atctacgtga caggaattgt cgctttggtg attgctgtga tcgtggtgct tgtatctgtc
     1621 cccggcatca tcgctggatt gggattgctt aaattcaagc cttgggcgcg tatcctggcg
     1681 attatcctgg ctttactgaa tctaccaggg ttccctatcg ggacagcgct gggcatatac
     1741 acgctttggg cgatgctgga tagcgaatct tctcagcttt tcaatgtaat agaaggttaa
     1801 atcaagagag caaaaccggt gttgtatatc attacagcaa caattctggt tttgctctca
     1861 ctctatttac gctttatcaa aaccaataca ttgccagctt cttttgaaga ctcgtagtgt
     1921 agttcgtcaa ccattttgcg catcaggtaa acacccaccc cccgtctttt taacttttgc
     1981 aaggggatat ttaccactgg ttcgggtact aaggcaaggt caaaaggtcg cccgtgatca
     2041 ttgagaatga ttttcagcgt tcctttgcca acctcaaccg aacaacgcat atcgcctatg
     2101 ccttcaccac cgtatgcgtg gtcgatgata ttactacagg cctcatctac cgctaactcg
     2161 acttcgtaaa tatcttggtc actaagaccc gcctcacggg ctgcttgtat gacaaatttg
     2221 ctgatttttt ccaggctttt gtattcagcc ggcaaattaa ctgtattcat taactcctaa
     2281 aagctaccaa ccgcggaggt gagatcagta aaaatgttaa aatacgagcc catccctact
     2341 aaatttagag aatcttggat ctcgtcccgt acattgacca gtaccaattc gccgcgctta
     2401 tatcgtttgc aggttttttg tgtatcaata agcacccacc aacccttact ggacataaag
     2461 gttacttcac tcatatcaag tacaatctta aaccgacccg aatcggtgat agatttaagc
     2521 gcctcctcca gctgaggggc ggtgctgctg tcgacgcgtc ctttggcttc gaccacatcg
     2581 cagcgtttat attcagtggt tgagatctcc atcggctgga atcctcctgg tttttagatg
     2641 gggtaatgtg aaattatatc atgcagtcgg tgggctaggg ctctcttcat cgctggattc
     2701 ccttttgccc catcgagagg ttaccttctc aagctcagtg ttgattaaat tcaaagcttt
     2761 gagaagttcc tctctggctt tttcctcggc ctcaccactg cgcacgcgct ggctcaagtc
//
LOCUS       DBNGSX010000045         4125 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_695306, whole genome shotgun sequence.
ACCESSION   DBNGSX010000045 DBNGSX010000000
VERSION     DBNGSX010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_695306"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..>4125)
                     /locus_tag="AC16CB_01440"
     CDS             complement(116..>4125)
                     /locus_tag="AC16CB_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3341157.1"
                     /translation="IYIINGTQPARTALVTLLLLWLGLALLRTMPDFKASLGNGQILT
                     NGSLSQPFGVVASAFRLGAAMVVMIIIYQALSNLRNRYPSRAAASLGLLAAVWLDAIL
                     FSLLTSSSAPILANLILILLGGNTLAVVTLWPLFSIYLGHFAVYFPETGARTPRLALD
                     LFSNTTELEARARYQISLLRTISQISQLIGKTSDPQILLERACQYLVSNRAYSLVWIG
                     MVHPARDGSQLEIHPAARAGRGRHIIEDIHTSRKGVSERSNPAETAAQSGQAFLIQDI
                     AQTTQDAPWQEAALQYGFLSSAALPMRHAGHTLGVLNIFGEGPNAFDREETDLLQELA
                     DELAHALISLEARQQQAILHTAVETMQDGLFITNIEGRILYANPAIAQLLEVSSQELE
                     GLDIRRVFPTNGQFLPFDKLRLAIERDEKADIDLELRLSDQRNVYFSIRASLAMDTIS
                     QPAYVVINLRDTTRHHQYEHQLLTLNRFTTELVQAHEVNILMDRLFVAGESLLQADAS
                     SFFLLQGDNHSIAQSFTHNLPALKLENLNSLNELLPDTIPYPTRQPIVIAEPQDKPGY
                     AARLENLTGGMQTLLLLPISLQDRQIGALSFFYRQVRRFTEEEIQLGLTLAHTLAISL
                     ENTRLYQAEKSQREFAEALARGAAALNNSLEFDRVLDQILDQTLDVVQCRSVNIMLIE
                     GDQVRVVRHLDKSDPSEAKRPVTGKALSLLLPTLQQMLSSGKPLVIPNTSQDPLWQQL
                     EQTTWIRSYAAAPLQVQGRVIGFLNMNSDEVGYFTAQIFPRLQAFADTSAAAIQNASL
                     YKESQMRGEELATLVEAAAVVSSSLDVNQVLQVVAEQMISLLNIRGCAISDYDPETKS
                     VKLISDFGPIEWLNNKERDKPYYLDEYPLTRQVLETGEPLLVRVDDPDLEANERGFME
                     RNHIKVSLMLPLVTRDRTIGLVELVDDRLDHSFSESEIELVQTLASHAAIAIENARLY
                     QHTQKYAEELEDRVRERTMELRNAKERIEHILVSVPDAIIVLDEDNRLIQANPSGDAL
                     IVQANEHGLDIFNSDFLRSLARQNTPTEKTILQLRDRAYQALASSLPIDEKKFGTVIV
                     FRDVTRFREIDQMKTQFVSDVSHELRTPLTNLSLYLDLLATTNDTAKNQRFLATLRRE
                     TQRLTSLIEDLLTISRLEADRVEINIEPVNLNHIVSDLAEDRRQMAANLGLNLTYTTH
                     PELPYALADARLLTQVLSNLLTNALNYTPPSGRIHLQTLMQDWNDETWITVEVTDNGL
                     GIPPEELEHLFTRFFRGFASQESSAPGTGLGLAISKEIIDRLNGRITVKSQPNQGSTF
                     TVWLQPIQSHSGLSD"
BASE COUNT          920 a          990 c         1177 g         1038 t
ORIGIN      
        1 aagctgtgat aggctccgct gaagggaggt ttcctcccac ttctctaatt cttgctttag
       61 cttcgctagt ttctggcggt cgtacatgct atctccttcg ctctgtgacg atgttttaat
      121 cgcttaatcc tgaatgcgat tgtatcggct gtagccagac agtgaaagtg ctgccttggt
      181 ttggttggct cttcactgtg atacgcccgt tgaggcgatc gataatctct ttcgagattg
      241 ccagccccaa tccggttcca ggagcgctgc tttcctggct agcaaaaccg cggaaaaagc
      301 gcgtgaagag atgttccaat tcttctggcg ggatgcccag gccattatcg gttacttcta
      361 ctgtgatcca ggtttcgtcg ttccaatcct gcatgagagt ttgtaagtgg atgcgccctg
      421 agggaggggt gtaatttagt gcattggtga gtagattgga aagaacttga gtgagcaggc
      481 gtgcgtcagc cagggcgtag ggcagttctg gatgtgtagt gtatgtcaga ttcagcccca
      541 agttagctgc catctgcctg cggtcctcag ccagatcgga aacaatgtga ttgagattca
      601 cgggttcgat attgatctct acccgatcgg cttcgaggcg cgaaatggtc aagaggtctt
      661 caattaatga ggtcaagcgt tgagtctcac ggcgcaacgt ggctagaaag cgttgatttt
      721 tagctgtgtc atttgtggtg gcgagcaaat ccaggtaaag cgaaaggtta gttagcgggg
      781 tgcgcagctc atgtgagacg tctgatacaa actgggtttt catctggtct atttcgcgaa
      841 aacgggtgac atcccgaaag acgatcacgg tgccaaattt cttttcatca ataggtaggg
      901 atgaggccaa ggcttgataa gcacgatctc gtaattgcag gatcgtcttt tcggttgggg
      961 tattctgcct cgccagacta cgtaaaaagt cggagttaaa aatatccaaa ccatgttcgt
     1021 tagcctgtac tatcagagca tcgccggatg gattggcttg gatcaggcgg ttgtcctcat
     1081 ccagcacgat gatggcatca ggaacagaga ccaggatgtg ttcaatgcgc tctttggcat
     1141 tgcgcagttc catggtgcgt tcacgcacgc ggtcctcaag ctcttccgcg tatttctggg
     1201 tatgctggta gaggcgggcg ttctcgatgg cgatcgctgc gtgcgacgcc agggtttgca
     1261 ccagctcaat ctcgctctcg gaaaaactgt ggtccaggcg atcatcgact agctcgacca
     1321 gcccaatcgt ccggtcccta gttaccaatg gaagcatcag cgacactttt atgtggtttc
     1381 gctccatgaa cccgcgctcg ttcgcttcaa gatcagggtc atcaacacgc accagaagcg
     1441 gctcccctgt ttccagtacc tgacgtgtta atggatattc atctagataa tatggcttat
     1501 ctctttcctt gttgttcagc cactcaatgg gcccaaaatc cgatatgagc ttgaccgatt
     1561 tggtttcagg atcgtaatcc gagatcgcac aacctctgat attcagcagg cttatcattt
     1621 gctcagccac tacctggaga acctggttga cgtccaggct ggaggagacc actgcagcgg
     1681 cctctaccag ggtagcaagt tcctctccgc gcatctggct ttctttatac aggctggcgt
     1741 tctggattgc cgcggcagat gtgtctgcga aggcttgcaa gcgggggaag atttgggctg
     1801 tgaagtagcc cacctcatcg ctgttcatgt tcaagaagcc aatcacgcga ccctggactt
     1861 ggagcggcgc ggcagcatat gaacgaatcc aggtggtctg ctcaagctgc tgccacaagg
     1921 gatcctgaga tgtatttggt atcaccaggg gtttaccgct gctgagcatt tgctgcaatg
     1981 tgggtagcaa cagggaaagc gcttttcccg tcacggggcg tttggcttcg cttgggtctg
     2041 atttatccag atggcggaca acccgtacct gatcgccttc gatcagcatg atattgactg
     2101 aacggcattg gacgacatcc agcgtttgat caaggatttg gtccagtacc cgatcgaatt
     2161 ccaggctgtt atttagtgct gctgctccgc gcgccagtgc ttcggcgaac tcgcgctggc
     2221 ttttctcggc ctgatacagg cgcgtgttct ccagggagat tgccagcgtg tgcgccaggg
     2281 tgagaccaag ctggatttct tcctcggtga atctgcgcac ctggcggtaa aagaacgata
     2341 aagccccaat ttgcctgtcc tgcagcgaga tgggcaggag caacaaggtc tgcatgcccc
     2401 ccgttaaatt ttctagtcgg gctgcgtatc ctggcttatc ttgaggttcg gctataacga
     2461 tgggctgtcg ggttgggtag gggatcgtat caggcagcag ttcgtttaag ctatttaggt
     2521 tttccagttt aagggcaggt aggttgtggg tgaagctttg ggcaatggaa tgattatcac
     2581 cttgcaagag aaagaaactg ctggcgtcag cctgtagtaa ggattcgccg gcgacaaaaa
     2641 ggcgatccat caggatattc acttcatgag cctggacaag ctcggtcgtg aaacgattaa
     2701 gcgtaagaag ttggtgctcg tattggtggt ggcgagtggt atctcggaga ttaatcacca
     2761 cataagcagg ttgggagatt gtatccatgg ctaatgacgc ccggatagaa aaatatacat
     2821 tgcgttgatc tgagagtctt agctctaggt caatatcggc tttctcgtct ctctctatgg
     2881 ctaaccgtag cttgtcaaaa ggcaggaatt gtccgttggt tgggaatacc ctgcgaatat
     2941 ccaggccttc taattcctgg gaactgacct caagcagctg cgcgatcgcc gggttggcgt
     3001 agagaattcg tccttctata ttggtgatga atagaccgtc ctgcattgtc tccactgcag
     3061 tgtgcaggat ggcttgctgt tggcgcgctt cgaggctgat cagggcgtgt gctagctcgt
     3121 ccgccagctc ttgcaggagg tccgtttctt cacggtcgaa cgcgtttggg ccttctccga
     3181 aaatattgag cacgcccagg gtatgcccgg catgccgcat ggggagagcg gctgatgaga
     3241 gaaatccata ttgaagggca gcctcttgcc agggggcgtc ttgtgtggtt tgggcgatat
     3301 cctggatcaa gaaggcttgt ccactctggg cagctgtttc agccgggttg ctgcgctcag
     3361 atactccctt tcgcgaggtg tggatatctt caatgatgtg gcgcccccgt ccggcacgcg
     3421 cggctggatg gatttcgagt tgggagccat cacgcgccgg gtggaccatg ccgatccaca
     3481 ccagtgaata agcacggttg gagacaaggt attggcaggc acgttccagt aagatctggg
     3541 ggtcgcttgt cttgccgatc agttgactga tctggctgat ggttcgtaaa aggctgattt
     3601 gataacgggc gcgcgcctca agctcggttg tgttggaaaa caagtccaga gctaggcgag
     3661 gcgtcctagc accggtttca ggaaagtaaa cagcaaaatg gcctaaatag attgagaaca
     3721 gaggccataa ggtgacgact gccaacgtat tgccccccaa aagaataaga atcaaattgg
     3781 ctagaatggg cgcgctagaa gaggtaagca gtgaaaacaa aattgcatcc aaccagacgg
     3841 ctgccagcaa ccccaatgaa gctgctgcac gactgggata gcggtttctc aggttgctta
     3901 acgcctggta gatgatgatc atgaccacca tcgcagcccc cagtcggaag gcagaggcca
     3961 ctaccccgaa cggctgagag agagacccat tggtgagaat ctgcccgtta cccaggctcg
     4021 ccttgaaatc aggcatggtg cgcagtaatg ccagccccag ccaaagaagt aacagggtaa
     4081 ctaaagcggt ccgcgccggc tgagtgccat tgataatgta gataa
//
LOCUS       DBNGSX010000046         3538 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1180477, whole genome shotgun sequence.
ACCESSION   DBNGSX010000046 DBNGSX010000000
VERSION     DBNGSX010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1180477"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..1014
                     /locus_tag="AC16CB_01445"
     CDS             52..1014
                     /locus_tag="AC16CB_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3341158.1"
                     /translation="MTETAVSQPKPGIASLTPDELETPPLTLGQLTWRRFRRHRMAIF
                     GAVTLILMILYCFGGMLVFSEADANFVETGNRLSAPSREHPFGTDTIGRDILVRTIYG
                     GQISILIGLTAMLVELLIGITFGALAGYFGGILDSILMRITEAVLVIPQIFLLLVMAK
                     YFAQDIPNIQFLGRTLSGSVLVIIFVIGISSWPYLARIVRAQFLSVKENDFILAARAT
                     GTSTRDIIFRHILPNSIAPIIVSATLSVAAAITLEAYISFLGLGVRPPTATWGNMLEG
                     AYNYVESAWWLWFFPGVLIALTVLSINFLGDGLRDALDPHSRAI"
     gene            1028..2017
                     /locus_tag="AC16CB_01450"
     CDS             1028..2017
                     /locus_tag="AC16CB_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019955731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3341159.1"
                     /translation="MADRILEVNDLRTRFHIAEGTVHAVNGISFYLEHGKMLGVVGES
                     GCGKSVTMLSLLRLLPMPPAEITSGQVLYLGRDLLQISEHEMETLRGKEIAMVFHDPM
                     TSLNPVLTVGRQITETLRAHMGMEKQMARERAIELLDQVGIPDSARRLDDYPHQFSGG
                     QRQRAMIAMALACVPSILIADEPTTALDVTIQAQIVELVQRLRANLDMAVIWITHDLG
                     VVAEMAERVLVMYAGFIVEEALVDDLYEDPRHPYTIALLAALPRIDRRRDKRLRSIPG
                     APPNLLVEPTGCPFAARCELAFDRCRTEMPPLVQINPNHQAACWVDVTTGKPR"
     gene            2037..3044
                     /locus_tag="AC16CB_01455"
     CDS             2037..3044
                     /locus_tag="AC16CB_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3341160.1"
                     /translation="MADDQSTSSTKPEALVQVRELKKYFPVKRGLIFEREVGAVKAVD
                     GISFDIRKGETLGLVGESGCGKTTAGRVILGLYPITDGSVTISGIQVDQASKQELQTL
                     RRKAQMIFQDPYASLNPRWTVNAIIGEPLRVHKLLPNDKARMDRVNELMLLVGLSPRL
                     INRFPHEFSGGQRQRIGVARALASSPEFIVCDEPISALDVSIQAQVVNLLEDLQDEFN
                     LTFLFIAHDLSMVRHICDRVAVMYLGVIVELTGKDELYENPLHPYTQALLSAVPIPDP
                     KKGRARQRTILPGDVPSPINPPSGCRFHPRCPIAKGHCSQIVPEWREISEGHWVACHE
                     V"
     gene            3118..>3538
                     /gene="arfB"
                     /locus_tag="AC16CB_01460"
     CDS             3118..>3538
                     /gene="arfB"
                     /locus_tag="AC16CB_01460"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HIR3341161.1"
                     /translation="MVEITPTLSLEDNELRFDFIRASGPGGQNVNKVASAIKLYFDVR
                     QSPSLPDEVKERLAKLACKRMTESGGLVIDARRYRTQEGNRLDATQRLVALIQKALEE
                     PTPRKKTRPSAAARAARLAEKKRRSMIKRERRSQPPDW"
BASE COUNT          731 a         1097 c         1008 g          702 t
ORIGIN      
        1 atcacctacg cctggctgga ccccaggata cgttataaat agcgagctat catgaccgaa
       61 acagcagtct cacaacccaa gcctggaata gccagcctga cgcccgacga gctggaaacc
      121 cctcccctga cccttggcca gctcacctgg cgccgcttcc gccggcacag gatggcaatt
      181 ttcggcgcgg tgacgttgat cttaatgatc ttgtactgtt ttggtggcat gctggtcttc
      241 agcgaagccg atgccaactt cgtcgaaaca ggtaaccgtc tttcagcgcc ctctcgcgag
      301 caccccttcg gcaccgatac cattggcagg gacatcctgg tccgcacgat ttatggcggg
      361 cagatctcca tcctgatcgg cctgaccgcc atgctggttg agctgttgat cggaatcacc
      421 ttcggggcac tggcgggcta cttcgggggc attctggata gcatcctgat gcgcatcacg
      481 gaggcggtgc tggttatccc ccagatcttc ttgctgctgg tgatggccaa atatttcgcc
      541 caagacattc ccaacatcca gttcctcggg cgaaccttaa gcggcagcgt cctggtcatc
      601 attttcgtca tcggcatctc cagctggccc tacctggcgc gcatcgtccg cgcccaattt
      661 ctctctgtca aagagaacga cttcatcctg gcagcgcgag caaccggtac gtctaccagg
      721 gatatcatct tccgccacat cctgcctaac agcattgccc ccatcatcgt gtcggctacc
      781 ctgagtgtgg cggcggccat caccctggag gcttacatca gcttcctggg gttgggcgtg
      841 cgccccccca ccgccacctg gggcaatatg ctggaaggcg cctacaacta cgtcgaatcc
      901 gcctggtggt tatggttctt ccctggcgta ctgattgcct tgaccgtgct gagcatcaac
      961 ttcctgggcg acgggctgcg cgacgccctc gacccccata gccgtgcaat atagccggga
     1021 gaataagatg gcggaccgca tcctcgaagt caacgacctg cgcactcgat ttcacatcgc
     1081 cgaaggcacc gtccatgccg ttaatggcat ttctttctac ctggagcatg gcaaaatgct
     1141 tggcgtggta ggcgagagcg gctgcggaaa atcagtcacc atgctgtccc tgctgcgatt
     1201 gctacccatg ccaccggctg agattaccag tgggcaagtg ctatatctgg ggcgtgatct
     1261 gcttcagatc agcgaacacg agatggagac tctgcgcggc aaagagatcg ccatggtctt
     1321 tcatgacccg atgacctcac tcaacccggt gttgaccgtc ggtcgccaga ttaccgaaac
     1381 gctcagggca cacatgggga tggagaagca aatggcccgc gaacgggcta tcgaactgct
     1441 tgaccaggtc ggcatccccg actctgcccg gcgtttagat gattacccgc accagttctc
     1501 tggagggcag cgccagcgtg ccatgatcgc catggccctg gcttgcgtgc cgagcatctt
     1561 gatcgccgat gaacccacca ccgccttgga tgtgaccatc caggcccaga ttgtcgagct
     1621 ggtgcagcgc ttgcgggcca acctggatat ggctgtcatc tggatcaccc acgacctggg
     1681 tgtggtggct gagatggctg agcgggtgct ggtgatgtac gcgggcttca tcgtcgaaga
     1741 agcgctggtg gacgacctgt acgaagaccc gcgccacccg tacactattg cgctgctggc
     1801 agcactgccg cgcatcgacc gccgccggga caagcgcctg cgctccatac ccggggcgcc
     1861 gcccaacttg ctggtagagc caacgggctg cccgtttgcg gcgcgctgcg aattggcctt
     1921 cgaccgctgc cggaccgaaa tgccgccctt agtacagatc aacccaaacc atcaggccgc
     1981 atgctgggta gacgtgacca ccggtaaacc gcgctaatcc tatttggaga tatcccttgg
     2041 ctgacgacca atccacttcc tctacaaaac ccgaagcact ggtgcaggtg cgtgaactga
     2101 agaagtattt tccagtcaag cgtggtctta ttttcgagag agaagtcggc gcggtcaaag
     2161 ccgtagatgg tatatcattt gatatccgta agggggaaac gctgggcctg gtgggcgaga
     2221 gcggttgcgg caagaccacc gccgggcgcg tcattttagg cctgtacccc atcaccgatg
     2281 gttcagtcac aatctccggg atccaggtgg atcaggccag caagcaagag ctgcaaacac
     2341 tgcgtcgcaa agcgcagatg atcttccagg atccctacgc ctcgctcaac ccgcgctgga
     2401 cggtcaacgc catcattggc gagcccttgc gggtgcacaa actgctgcct aacgacaagg
     2461 cgcgcatgga ccgcgtcaac gagctgatgc tgctggtggg gttaagccca aggttgatca
     2521 accgcttccc gcatgaattc tccggcgggc aacgccagcg catcggtgtg gcgcgcgccc
     2581 tggcatccag cccggaattc atcgtttgcg atgagccgat ctccgccctg gatgtctcca
     2641 ttcaggcgca ggtggtcaac ttgctggaag acctgcagga cgagtttaac ctgacattcc
     2701 tgttcatcgc ccacgattta agcatggtgc ggcatatctg cgaccgggtg gcggtgatgt
     2761 acctgggtgt aattgtcgag ctgaccggga aagacgagct atacgaaaac ccattgcatc
     2821 cttataccca ggcgctgctc tcggcagtac ccattcccga cccgaagaag ggtcgcgccc
     2881 gccagcgaac catcctgccc ggagatgtgc ccagcccgat caatccgccc tccggctgcc
     2941 gctttcaccc gcgctgccca atcgccaagg ggcactgctc gcagatagtc cccgaatggc
     3001 gagagatctc cgagggtcac tgggtggctt gccacgaagt ttgacaacac ctgctagaag
     3061 acagcttaca actaatcgag ggggcagatg gctaccacca gatgatatac ttgcctcatg
     3121 gtcgagatca cacctacttt gagcctggaa gacaatgagc tgcgcttcga cttcattcgc
     3181 gcctccgggc cggggggaca gaatgtcaat aaagtcgcca gcgccatcaa gctgtatttc
     3241 gatgtgcgcc aatcgccgtc cctgcccgac gaggtgaagg agcggttggc gaagctggca
     3301 tgcaagcgca tgacagagag tggtgggctg gtgatcgacg cccgtcgcta ccgcacgcag
     3361 gagggaaacc ggctggatgc cacccaaagg ctggtggcgc tgattcaaaa ggcgctcgaa
     3421 gagcctaccc cgcgcaagaa gacccgcccc agcgccgccg ctcgggcagc acgtctggca
     3481 gaaaagaagc gccgcagcat gattaagcgc gaacgccgct cccaacctcc ggattggg
//
LOCUS       DBNGSX010000047        12320 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1318914, whole genome shotgun sequence.
ACCESSION   DBNGSX010000047 DBNGSX010000000
VERSION     DBNGSX010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12320)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12320)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12320
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1318914"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /locus_tag="AC16CB_01465"
     CDS             complement(<1..1213)
                     /locus_tag="AC16CB_01465"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR3341162.1"
                     /translation="MKMWRFKHLFVLGLVLLLVSLACGIGGSAPTEAPPPSEEPVEVT
                     EPPATEAPEMTEPPEPTSGAVSILQDVKKAVIQIESQGTFLDPEFGLVLNGAGRGSGF
                     IIDPSGIAVTNNHVVTGAALLKVWVGGESSPRNARVLGVSECSDLAVIDLEGDGYEYL
                     EWFDGAIDVGMDMYIAGFPLGDSEYTLTRGVISKATANGETSWSSVDSVVEYDATSNP
                     GNSGGPVVDRDGKVLAVHYAANRDTRQAFGISRDVGESIINELKTGKNVDTIGVNGTA
                     VSNEDGSLTGVWVASVQSGSAADKAGVEAGDIIMSMENLLLATDGTLSQYCDILRSHQ
                     PSDTLSLNVLRWASGELLEGQLNGRELEVTSTFELSDGTEGGSTAGSGTDVVNPNASA
                     SGEYYYYTDFDG"
     gene            complement(1463..1972)
                     /locus_tag="AC16CB_01470"
     CDS             complement(1463..1972)
                     /locus_tag="AC16CB_01470"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3341163.1"
                     /translation="MSTSEIPAEKAVKYCQRCGASMTHQQRYGRERPVCPRCEWIFFP
                     DPKVASAVLVEQDGKILLVRRALDPEKGKWTLPAGFIDAGEDPARAAERECLEETGLL
                     VQVKELLTVLYGQEHPHGAHIIIFYRAELVSGSLCPGDDVDAVEFFPRHQLPPLAFQT
                     THKVLSQKN"
     gene            complement(1983..2855)
                     /locus_tag="AC16CB_01475"
     CDS             complement(1983..2855)
                     /locus_tag="AC16CB_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR3341164.1"
                     /translation="MTTNLTPRDWEALSAYLDGQLAPKEQQLLEARMRSRADLRAAME
                     ELRRTRAVLRAHLEVRAPRNFTLTPEMAGVRPRSRPTVAFFPALRLTSAISSLLFVLV
                     ALSELLIGGRLALPMAAGEPQREAMSEAAPQEPVLEPSAAPAMAVEAPAAEEVEATPQ
                     PVAKAVEAPTPSLGLELAQPIVIETETVELPPFAGGMGGGDGADETQLDEEALSQMAP
                     IATATPLPTLTFTPQPSPSPTVMEQPTAPAPDRARFARFPEWTVLRWVEITLAIVGLS
                     TGLLAIILHRRGRS"
     gene            complement(2852..3460)
                     /locus_tag="AC16CB_01480"
     CDS             complement(2852..3460)
                     /locus_tag="AC16CB_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3341165.1"
                     /translation="MDEAALISDARQGDLNAFNRLILAYQDLVYAQAYRMMGEPEAAE
                     DATQEAFISAFRSIRSYRGGSFRAWLLRIVTNACYDELRRRKRRPTTPLEPMDAEDEE
                     IESPHWLADPSESPEDRTERLELDQAIQNCLEDLPPDFRSVVVLVDIQGFDYIEAAEV
                     IKKPVGTVKSRLSRARLRLRDCLQGWWELIPAQFRLVNEGKP"
     gene            complement(3465..3848)
                     /locus_tag="AC16CB_01485"
     CDS             complement(3465..3848)
                     /locus_tag="AC16CB_01485"
                     /inference="COORDINATES: protein motif:HMM:NF022700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3054 domain-containing protein"
                     /protein_id="HIR3341166.1"
                     /translation="MKDNRLLGVLAGDVITLALVTLAGFARHDELDNANFRMLTTFIP
                     LVLAWLLVAPHLAVFDLQRLSDPRQLWRPFWAMVLGAPFAGWLRGVMLNSPVLPIFVF
                     VLGGVSALALLAWRSAYWLIFARRS"
     gene            complement(3898..5676)
                     /locus_tag="AC16CB_01490"
     CDS             complement(3898..5676)
                     /locus_tag="AC16CB_01490"
                     /inference="COORDINATES: protein motif:HMM:NF015557.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR3341167.1"
                     /translation="MTYEISALLFILGIAVVLFSFEWVSVDIVAIGILLSLVLLGLVP
                     TEDAFAGFGNETVVMILCLFILTAALVRTGVIDIIGRALLSRINNNPSQITIIVMGAA
                     AVMSTFMSNTASAAFFAPVVMGLSRRLRISASRLLMPVAFAAILASSMTLVSTSTNLV
                     VNGLMLQYGMKAMGMFELTPVGIPILLVGLVYMASIGKRMIPDRSHPEELTQEFNLRP
                     YLTEIVILPNSSLAGKTLVESGLGREYDFTVLRITRDDDVHLVPHADVLLEQGDVLLV
                     EGHRDQLFKIKDWVGIGVAEEVALSDPDLQTKDYQLAEVILLPRSPLIGRRLTGVDLR
                     EQYGVQVLAINRHESTLRSKIGQIPLQMGDVLLVQGPRFNLSTLEKSNTFRVLGTFEN
                     MQPNHSRAWLAMAIFVGALLLAIFDLVSLPVAVLLGTLVAFITRCITPEEAYREVEWK
                     AVILIGSMLSLGGAMEFTGTSKFLALQIVRLVGETNPVWLLTGFFMLTVIMTQPMSNQ
                     AAAAVVLPVALQTAIQLGLNPRTFAMMIAVAASTSYITPLEPACLIVYGLGRYRFTDF
                     LKVGSLLTVFIYIVAILLVPRVWSLY"
     gene            5970..6701
                     /locus_tag="AC16CB_01495"
     CDS             5970..6701
                     /locus_tag="AC16CB_01495"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3341168.1"
                     /translation="MLERAQHCCSFHTVTLIAMALDTSRIQALCFDVDGTLSDTDDQF
                     VLRLTRMLRPVSFLFPRRDPHSFARQVVMATESPGNYLLGLPDRLGFDHNLAALGDWI
                     YRIGLGRGKSRFLLVPGVRELLNRLQPRYPMAIVSARGARSTRAFLDQFELSHFFKCI
                     VTAHTCSHTKPYPDPIQFAAQEMGVPPQACLMIGDTTVDMRAGKAAGAQTLGVLCGFG
                     VESDLRRAGADLILPTTSMLTDVID"
     gene            6790..7770
                     /locus_tag="AC16CB_01500"
     CDS             6790..7770
                     /locus_tag="AC16CB_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341169.1"
                     /translation="MVAKTRWVGVVFVLSLFLSWMPSLAQEITQPPNERRYYPQRGHW
                     VMGEFLEVYQSVPNPAEIYGYPITEAFQEQTLGRIVQYFEKARFELIPENPPELRVKI
                     TELGSYFYTTAGQELPLPANFPACLFFQETGKQVCYAFLEYFEANGGAAQFGYPLSNF
                     ETQDGLIVQYFQRARFEWHPEFPPGKRVKLTELGYRYFKKIGEDPARLLPAPWLAPGS
                     DIPQPVLSLRVRAFPLHAVLTQNAAQSIFIIVQDQNLLPVSNAQVTLIIRLPSGKEQR
                     VIVPGTTDRFGVIKYSFDYTDQPTGVAQVIVNAIYETFHKQTITSFRIWW"
     gene            complement(7840..9525)
                     /locus_tag="AC16CB_01505"
     CDS             complement(7840..9525)
                     /locus_tag="AC16CB_01505"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR3341170.1"
                     /translation="MRSKDEAFYFQAINLPPIGKGGGLRSGFLAGGSSLLKTLFFFIL
                     GVMLVIALGGCTSTPKNTSNPSAMTQTSKPVLPANTTMPSGGSTPVAGFEDQSPAILP
                     AAQAELALLPDLTRYDIDLVIDSAAQTFSGQARVDVTNTEGQPLESLYFRLLPNGGQS
                     YGNGSLTVSDTRLIGDTADTILSLNNTVLEVKLPQVLQPGERVQVEFDFQGIVPLDFG
                     GDASPYAYGIYNKSEGVLALSGWYPILAVYDEGGWHLDPISPIGDSVFSEMALYTVRV
                     TMPSDLVLVSTGVEISRESLGATTKYLLVSGPMRDFFMIASPDFQVVSQIVDGTRVNS
                     YYFSEDEYAGGVGLSVSAESLRVFNQRFGKYPYIELDMVQAPMRNALGVEFPGIYLIG
                     ASLYDEPDTPDFAVTVAHEVAHQWWYSLVGNDVFAEPWLDEALSTYSSGIYYQDVLGD
                     GAYSGLASYWQSRYDTLIQDGEDDVITRDLKYFESLGVPRVYSGVVYRKGALFFKALR
                     EAIGDSAFFDALQGYYLDNKYGVGNGDELLSAFEQASGQELDDLYNEWLYTSK"
     gene            complement(9509..10450)
                     /locus_tag="AC16CB_01510"
     CDS             complement(9509..10450)
                     /locus_tag="AC16CB_01510"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020488092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3341171.1"
                     /translation="MDAAPTTPAVSSSTPTMLASSTLAPVETPTASLTPFLVGPSDTP
                     SPTPEPTSENPVVFAVIGDYGSGNRTEGYVADLVNSWQPDFIITTGDNNYPDGSEDTI
                     DATIGQFYHNFIYPYVGDFGEGADQNRFFPSLGNHDWITDDARPYLDYFTLPGNERYY
                     DFTWGPVHFFALDSDSNEPDGVGRSSKQAEWLQASLAESTLPWNIVYMHHPPYSSGLH
                     GSVTWMRWPFKDWGATAVLAGHDHDYERLIVDDMLYFVNGLGGGGIYPFSNRLEGSQV
                     RYNDDYGAMRVKATTKKIMFEFISRDGEVIDSYEIER"
     gene            complement(10503..11801)
                     /locus_tag="AC16CB_01515"
     CDS             complement(10503..11801)
                     /locus_tag="AC16CB_01515"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR3341172.1"
                     /translation="MGIAGVHAVCVQRYDPSYRRASCSTCFCPGDPIPLEVVQPSATA
                     EPPVQESMTQPAPPTTLPSESPADTAPPAGPAAVLPEAADYLWERVVGGLVNPIGLEN
                     AQDGSGRLFIVEQDGRILIWKDGTLLDAPFLDIRDRVGNRGSEQGLLGLAFHPRYTEN
                     GYLYVNYTDTKGDTHIARFTVSEVNPDQADPTTEKTLLVVSQPFGNHNGGVVVFGPDG
                     YLYLGLGDGGSGGDPQGNAQSTQTLLGKILRIDVDSGDPYGIPPDNPFAGGGGLPEIW
                     AYGLRNPWRISFDRLTGDLYIGDVGQNQWEEIDFLSSGAQPGANFGWDYREGFHPFEG
                     AYQGNDLIDPLIEYDHSQGCSVTGGVVYRGINLQAWQGVYIYGDYCSGNVWGLLRNSD
                     GSWQNGLLFQTNSRISSFGVDEAGEVYLVDHRGDIYHLTQ"
     gene            complement(11812..12174)
                     /locus_tag="AC16CB_01520"
     CDS             complement(11812..12174)
                     /locus_tag="AC16CB_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341173.1"
                     /translation="MSSNRNRSITPSKGGGFFQDLAVRIKLILRLMSDARVSPWLKVL
                     PLGSLIYLIVPDIAPGPLDDAAVIWLGTYLFVELCPPEVVQEHLRALTQEIPGEYRDP
                     LANEGEIIDAEFTEEEKS"
BASE COUNT         3103 a         3396 c         3025 g         2796 t
ORIGIN      
        1 taccatcgaa atcggtatag tagtaatact cgcctgaggc gcttgcgttg ggattgacca
       61 catctgtgcc cgatccagcc gtgctgccgc cttccgtgcc atcggaaagc tcaaatgtcg
      121 aagtcacctc aagctctcgt ccattgagct gcccttccaa aagttcgcca gaagcccaac
      181 gcagcacgtt cagcgagagt gtatcgcttg gctgatggga gcgcaggata tcgcaatatt
      241 gggacagcgt cccatccgtc gccagcaaca gattttccat actcatgatg atatcgccag
      301 cttccacacc ggctttatca gcggcagagc cagattgcac tgaagctacc cacacaccag
      361 tcaatgagcc atcctcgttg ctcactgccg tgccattcac gccgattgtg tccacatttt
      421 taccggtctt gagttcattg atgatgcttt cgccaacatc gcgcgaaata ccaaatgctt
      481 ggcgggtatc acgattagca gcataatgca cggcaagaac tttgccatct ctgtctacca
      541 ctggcccacc ggagttccct ggattactgg tggcatcgta ttcaactaca gaatcaaccg
      601 aagaccagga agtctctcca ttcgctgtcg ctttggagat gacacccctt gtaagggtgt
      661 actccgagtc gcccagaggg aatccggcga tatacatatc catgcctaca tcaatcgcac
      721 catcaaacca ttccaggtat tcgtatccat cgccttccag gtcgatgaca gccaaatcag
      781 agcactcgga cacccccagc acgcgcgcgt tacgaggaga gctctcgcca ccaacccaaa
      841 ctttcagtaa agccgcgcca gtgacgacgt gattattcgt gacggcgata ccagaaggat
      901 caatgataaa tcccgagccg cgacctgccc cattcagcac caatccaaat tccggatcaa
      961 gaaaggtacc ctgagattca atttgaatca ctgctttttt aacatcttgc agtatactaa
     1021 ccgcccctga agtcggctca ggtggctcgg tcatttctgg agcttccgtg gcaggcggct
     1081 ccgtcacttc aactggctcc tcactgggtg gcggagcttc tgtaggagca ctccctccaa
     1141 tgccacatgc taggctcact agaagtaaga ctagacctaa cacaaacagg tgcttgaatc
     1201 tccacatctt cattgcttct tctccctata agcgaattga accaggaaaa ctaattactc
     1261 ctagctaaat ataggcgtag tttaccataa ccgcactgcc caacacgcat atagaaactg
     1321 cgcctattaa atttgtaaag caacgccaga ctttatcact gcctggactc atctcaagct
     1381 atcgtggtat tgacaccaac ccacacacga gcagtttaat gtctctgcat attcgtctta
     1441 ggctgaaaac agccagcatc agtcagtttt tttggcttaa tactttatgg gtggtctgaa
     1501 aagccaatgg aggtaactga tggcgtggaa agaattccac ggcatctaca tcgtctccgg
     1561 ggcacagcga gcccgagacc aactccgccc ggtagaagat gatgatgtga gcaccgtgtg
     1621 gatgttcttg accgtaaagc acagtgagca gttcttttac ctgcactagc agacctgtct
     1681 cttccaggca ctcacgttcg gcggcacgag cagggtcttc accggcatcg atgaagcctg
     1741 ccggcaatgt ccactttcct ttttctgggt caagggcgcg ccgcacgagc agaatctttc
     1801 catcctgctc tacaagaact gcagaagcta cctttgggtc agggaagaag atccattcac
     1861 aacgtggaca aaccgggcgc tcacggccgt aacgttgttg gtgggtcata gatgccccgc
     1921 agcgctggca atacttaacc gctttctcag caggtatctc gctggtactc acaagatttc
     1981 cttcatgaac gtccacgtcg atgaaggata atggccagca aacctgtgct caaacccacg
     2041 atagccaggg tgatttcaac ccagcgcaaa accgtccatt ctgggaaacg agcaaaacga
     2101 gccctgtccg gggctggcgc ggtgggctgt tccataactg ttggggacgg cgaaggttgt
     2161 ggggtaaagg taagtgtggg caagggtgta gcagtcgcta taggagccat ttgcgagagc
     2221 gcctcttcgt ctaattgggt ttcatcggct ccgtctccgc cgcccatacc gcctgcaaat
     2281 ggaggcaact cgacggtctc agtctcgata acaatgggct gggcgagttc cagtccaagg
     2341 ctgggagtag gggcttccac agccttggcg actggctgag gcgttgcttc aacttcttca
     2401 gctgctggag cttctacagc catcgctgga gctgccgagg gttcaagaac aggttcttga
     2461 ggtgcggctt cagacatcgc ttcacgctgc ggctcaccag ccgccatagg taaagccaga
     2521 cgaccaccaa tcagtagctc actcaatgcg acgaggacaa acagcaagct agatattgcc
     2581 gaagtcaggc gcaacgctgg aaagaaggcc accgtcgggc ggctgcgagg tcggacaccg
     2641 gccatctcgg gtgtcagggt gaaattacga ggggcgcgca cctctagatg cgctcgcaat
     2701 accgctcgag tccggcgcag ttcctccatg gcagcgcgta aatctgccct cgatcgcatc
     2761 ctggcttcga gaagctgctg ttccttcggg gccagttgcc catccaggta agccgataag
     2821 gcttcccagt cacgaggggt aagattagtc gtcatggttt accttcattc accagacgaa
     2881 attgggcggg aataagttcc caccaacctt gcaaacagtc gcgtaaacgt aagcgtgcac
     2941 gcgagagacg gctcttcacc gtccccacag gctttttaat cacctcagcg gcctcaatat
     3001 aatcaaatcc ctgtatatcc accaatacaa ccaccgaacg gaaatccggg ggtaagtcct
     3061 ccaggcaatt ttggatggct tgatccaatt ccaggcgctc agttcgatcc tccggcgatt
     3121 cgctggggtc agctaaccaa tgtggcgatt caatttcttc atcctcagca tccattggct
     3181 ccaggggagt cgtcgggcgg cgcttacgtc ggcgcaactc atcataacag gcattggtga
     3241 caatgcgcag caaccaggct cggaacgagc cgccgcggta agagcggata ctgcggaaag
     3301 ccgagataaa tgcttcctgg gtggcgtcct cggcggcttc tggctcaccc atcatgcggt
     3361 atgcttgggc gtagaccaga tcctggtatg ccagtatcag gcggttgaaa gcattgagat
     3421 caccctgcct ggcatctgag atgagcgcgg cttcatccat gcaatcagct ccgccgagca
     3481 aaaatcaacc aatatgcgct gcgccaggca agcaatgcca gggcgctcac tccacctagg
     3541 acgaacacaa atatgggtaa gaccggtgaa ttcaacataa cgcctcgcag ccatcccgca
     3601 aagggtgccc ccagaaccat tgcccagaag ggtcgccata actgcctggg gtcgctgagg
     3661 cgctgtaggt caaaaactgc caggtggggc gctaccagca gccaggcaag cacaagcggg
     3721 atgaaagtgg tcagcatgcg aaaattagcg ttgtctaact catcgtgcct ggcaaaacct
     3781 gccagtgtta ccaatgccag ggtgatcaca tccccagcta atacacccag cagacggtta
     3841 tccttcatga atcatatcct aacccaaagc gatcaaaggg acaagggaga tgcgctacta
     3901 atacaatgac catacgcgag gcaccagcag aattgccaca atatatatga acacggtcag
     3961 caacgatccc actttgagaa agtcggtgaa acgatagcgc cccaacccat atacaatcaa
     4021 acacgcgggt tccagcgggg tgatatacga tgtgctggca gccactgcga tcatcatcgc
     4081 aaatgtgcga ggatttagcc ctaattgaat ggcagtttgc aaggctaccg gcaagaccac
     4141 cgcggcggca gcctggtttg acatgggctg agtcattatc acggtcagca tgaaaaagcc
     4201 agtcaacaac cagactggat tcgtctctcc gaccaagcgc acaatctgca atgccaggaa
     4261 tttggaagtg ccagtaaact ccatggcacc accaagagag agcatacttc caatcaagat
     4321 gaccgccttc cattctactt cacggtaggc ttcctcggga gttatgcagc gtgttataaa
     4381 agccaccagt gtacccagga gcacagccac tggcaatgag accagatcaa aaatcgcaag
     4441 taacagtgcg ccgacaaaga tcgccatggc taaccatgca cggctgtggt tgggctgcat
     4501 gttctcaaag gttcccagga cgcgaaacgt attggatttc tcaagcgtgg acaagttaaa
     4561 gcgcggacct tgtaccagca gcacatctcc catttgcaat ggaatttggc cgattttgct
     4621 gcgcaaggta gactcatggc ggttgatagc caagacctgt acaccgtact gctcacgcaa
     4681 atcgacacca gttaatcggc gaccgatgag tggtgagcgt ggtaacagga tcacctcggc
     4741 aagttgataa tctttagttt gtaaatccgg atcagaaagt gctacttctt cagccacgcc
     4801 gatcccaacc caatccttta tcttgaagag ctgatcgcga tggccttcaa cgaggagcac
     4861 atccccttgt tccaaaagta catccgcatg aggcaccaga tgcacgtcat catcgcgcgt
     4921 gatgcgcaat accgtgaaat catactctcg acccagacca gattcaacca acgttttccc
     4981 cgccagtgaa gaattgggca gaatgacaat ttcagttagg taagggcgca aattgaattc
     5041 ttgagttagt tcttcaggat gactccgatc agggatcatt ctcttcccaa tcgacgccat
     5101 gtagaccaag ccaaccagga gaatgggaat ccccactggg gttagctcga acatgcccat
     5161 cgctttcata ccatactgca gcattagccc gttgacaacg aggttcgttg aagtgctgac
     5221 gagggtcatc gaggaagcca agatagctgc aaacgccaca ggcataagca atcgggaggc
     5281 gctgattcgc aaccttcttg atagccccat cacaactggc gcaaagaatg cagctgaagc
     5341 tgtgttactc atgaatgtac tcatcactgc tgcggcgccc attacaatta tggttatctg
     5401 gctggggtta ttgttgattc ggctaagcaa agcgcgtcca ataatgtcga tgacccccgt
     5461 gcgtaccagg gcagccgtca agatgaacaa acacaagatc ataaccaccg tctcgttgcc
     5521 aaaaccagca aacgcatcct cagtaggcac caacccgagc agcaccagtg acagcaaaat
     5581 gcctatggca acaatatcga cagacaccca ttcaaaggaa aacaatacca cagcgatacc
     5641 taaaatgaag agcagggcgc taatctcgta agtcatgcta cacttgaggc aattcttgag
     5701 tacggctcac cgaatcaact ttttgttacg gtgcaatggg attttctata aaagagggaa
     5761 gtttctctcg ccacgacaac tttattgatc agaacttgac tttgaaataa tacaggctga
     5821 tgtgataata ttgggatttt actataatcc tcgtttagct gcgccacttg ttggctagac
     5881 gtaattgtga agattaagat ctgaaaaatg tctgaccttc tccaaaaaat cggcatgtct
     5941 tgcctgacgc tgttaattgc agcctgttta tgcttgagcg cgctcagcat tgctgcagct
     6001 ttcatactgt tacgttgatt gccatggcct tggacacttc ccgcattcaa gctctttgtt
     6061 tcgacgtaga tggcacattg agtgatactg acgaccagtt tgtgttacgg ctgactcgca
     6121 tgcttcgccc agtgagcttt ttattcccca ggagagatcc acattccttc gccaggcaag
     6181 tggtaatggc aacagaaagt cctggtaact acctgcttgg gttgcccgat cgtttggggt
     6241 ttgaccataa tttggcagcc cttggcgatt ggatatatcg catcggctta ggaagaggga
     6301 aaagccgttt cttgcttgta ccaggcgttc gcgaattgct caatcggctc cagccccgct
     6361 acccgatggc gatcgtcagc gcacgtggag ctcggagcac gcgcgccttt ctagatcaat
     6421 ttgaactctc ccattttttt aagtgcattg tgactgcgca tacctgttcg cataccaagc
     6481 cgtaccctga cccgatccaa tttgccgccc aagagatggg ggttcccccg caagcatgct
     6541 taatgattgg agatacgacg gtggatatgc gcgctggaaa agccgctggt gcgcagacgc
     6601 taggcgtcct gtgtggattt ggggtggaga gcgaccttcg tcgcgcgggt gcagatttga
     6661 tcttacccac aacatctatg ttgaccgacg ttattgatta accgccaggg atggggggtg
     6721 acaaaatagc aaaccgaata cctggcatat ggtattgcta tttcgcaata ttttgcatat
     6781 aatcatttta tggtcgccaa aacccgctgg gtaggggtag tatttgtgtt gtcacttttc
     6841 ctgagctgga tgccttcact cgctcaggag atcacccaac ctcctaatga gaggcgctac
     6901 tacccccaac gcgggcactg ggtcatgggg gaatttttag aagtttatca gagcgtcccc
     6961 aacccggcag aaatttacgg atatccaata accgaggcat tccaggaaca aaccctgggg
     7021 cgcatcgtgc aatactttga aaaagcgcgc tttgagctaa ttcctgagaa cccgccggag
     7081 ctgcgtgtca agattactga gctgggcagt tatttctaca cgaccgcagg gcaggaactt
     7141 cccttaccgg caaatttccc tgcctgcctc ttcttccagg agacaggcaa gcaggtctgt
     7201 tatgcattct tggaatactt cgaagccaat ggtggggcag ctcaattcgg ttacccactc
     7261 tccaatttcg agactcaaga tgggctcatc gtgcagtatt tccagcgtgc ccgctttgag
     7321 tggcatccag agttccctcc aggaaagcgt gtcaaactca ccgaacttgg ataccgctac
     7381 ttcaagaaaa ttggagaaga ccccgcccga ttgctgccag caccatggtt agcaccaggg
     7441 agcgatattc cccaaccagt gcttagcttg cgcgtacggg ctttcccgtt gcacgctgtc
     7501 cttacgcaga atgccgcgca gagtattttc atcatcgtcc aggatcaaaa tctgcttccc
     7561 gtctctaatg cccaagtgac tctgatcatc aggctgccct ccggaaaaga gcagcgggta
     7621 attgtgcccg gcactacaga cagatttggg gtgatcaagt attcctttga ttacaccgac
     7681 caaccaacag gcgtggctca ggtgatcgtc aacgccatct acgagacatt ccacaagcaa
     7741 accatcacat cttttcgaat atggtggtaa gaacatccac agaatgactc agccccacag
     7801 aataatctat gggtgagtta ttttgatcgt ttgtgttccc tatttggatg tatataacca
     7861 ctcattgtac agatcatcca gctcctggcc tgaggcttgc tcaaaggcag aaagcaattc
     7921 atcgccgtta ccgaccccgt atttattgtc caaataatag ccttgtaagg catcaaaaaa
     7981 cgctgaatcg ccgatcgcct cccgtagcgc cttgaagaac aaggctccct ttctataaac
     8041 aactccacta tagacacgag ggacacctaa gctctcaaaa tatttcaaat cccgggtgat
     8101 cacatcgtct tcgccatctt ggatcagcgt gtcatagcgg gattgccagt aagatgccag
     8161 accactatat gcgccatccc ctagcacatc ctgataatat atccccgacg aatatgtgct
     8221 tagggcttca tccagccatg gctcagcaaa tacgtcgtta cccaccaggc tgtaccacca
     8281 ttggtgcgct acctcgtggg caacggtcac tgcgaagtcg ggtgtatccg gttcatcgta
     8341 tagcgatgcg ccgatcaggt atatccccgg aaattctacc cccagggcat ttcgcatcgg
     8401 cgcctggacc atgtccaact ctatgtatgg gtacttgcca aaccgttgat tgaacacacg
     8461 caaagattct gcgctgacag ataaacccac cccacctgca tactcatcct ctgagaagta
     8521 atacgaattg actcgggtgc catccactat ttggctcacc acctggaaat cgggactggc
     8581 tatcatgaag aaatcccgca ttggcccgct gaccaagaga tattttgttg tagccccaag
     8641 actttcacgg ctgatctcta cgcctgttga cacaaggacc agatcgcttg gcattgttac
     8701 gcgaactgtg tagagcgcca tctcgctgaa gaccgaatca cctataggcg aaatcggatc
     8761 caggtgccag cccccctcat cgtatactgc caggataggg taccagccgg agagtgccag
     8821 caccccttcg cttttattgt agatgccata agcatagggt gacgcatcgc ctccgaagtc
     8881 aagtggtacg atcccctgga aatcgaactc gacctgaact cgctcaccag gctgcagcac
     8941 ttgtggtaac ttcacttcaa gcaccgtgtt attaagcgac aggattgtat cggctgtatc
     9001 tcctatcaat cgggtatctg aaacggttag cgagccattc ccgtagctct gaccaccatt
     9061 gggtaacaga cggaaataca agctttctag tggctgcccc tcagtattgg tcacgtccac
     9121 acgcgcttgc ccactgaagg tttgagctgc ggaatcaatg accaaatcga tgtcatagcg
     9181 cgtcagatca ggtagaagag ccagttcagc ctgtgccgct gggagtatgg cggggctttg
     9241 atcttcaaac ccagctacag gtgtactgcc gccagacggc attgtggtat tcgctggcag
     9301 gactggtttg ctcgtctgtg tcatcgccga gggattggat gtattctttg gagtggatgt
     9361 acagcctcca agagctatca ccaacatgac ccccaagatg aaaaaaaaca gggtttttag
     9421 caagctcgaa ccacctgcca gaaaccctga acgcaaccca ccccccttcc caatgggagg
     9481 gagatttatg gcttgaaaat aaaacgcttc atctttcgat ctcatagcta tcgatgacct
     9541 ccccgtcacg tgaaataaac tcgaacatga tcttcttagt ggtagctttc acccgcatcg
     9601 ccccataatc gtcattgtag cgcacctggc tgccctccag ccggttgcta aatggataga
     9661 taccaccccc gcccagacca ttgacaaaat acagcatgtc atcaacgatc agacgctcgt
     9721 agtcgtggtc atgtcctgcc aggacagcag tcgcacccca atctttgaaa ggccagcgca
     9781 tccatgtcac cgagccgtgc aggcccgaag aatatggtgg atggtgcata tagacgatat
     9841 tccaaggcaa ggtcgactcc gccaggctag cttgcagcca ttctgcttgc ttagaactcc
     9901 tccccacacc gtccggttcg tttgaatcgc tatccaaagc gaagaagtgc accggtcccc
     9961 atgtgaagtc ataataacgc tcgttacccg gcaatgtgaa ataatccagg taggggcggg
    10021 cgtcatcggt tatccagtcg tgatttccca ggctggggaa aaagcggttc tggtcagctc
    10081 cttcaccgaa atctccaaca taaggataga tgaagttgtg atagaactga ccgatagtgg
    10141 catcgatagt atcctctgat ccatcgggat aattgttgtc gccagtggtg ataatgaaat
    10201 caggctgcca gctgttcacc agatctgcca cgtatccttc agtgcgattc ccagagccgt
    10261 aatcgccaat cacagcgaag accacggggt tctcagacgt cggttcaggc gtggggctgg
    10321 gagtatccga agggccaacc aggaatggcg ttagagaagc tgtcggagtc tccactggag
    10381 caagcgtgga gcttgccagc atggtcggcg ttgaggaaga tactgctggg gttgtcggtg
    10441 ctgcatccat caagccacac cccatcaggc ttagtatcca tatcaacccc aggatttgta
    10501 ttttactggg tgagatggta tatatctcct cgatgatcta ccagatacac ctctcctgct
    10561 tcatcaactc caaaagaaga gatacgagag ttggtttgga aaagcaagcc attctgccat
    10621 gagccgtccg agttgcgcag caagccccac acattcccgc tgcaataatc accatatata
    10681 tatacgcctt gccacgcctg gaggtttatg ccccggtaga ccacaccgcc tgtaaccgag
    10741 cagccctgtg agtggtcgta ttcgatcagc ggatcaatca ggtcgttacc ttggtaagca
    10801 ccctcaaatg ggtgaaaccc ctcgcggtaa tcccaaccga aattcgctcc tggctgcgcg
    10861 cctgaagaca agaagtcaat ttcctcccac tgattctgcc ccacatcccc gatgtataaa
    10921 tctccagtga gccggtcaaa gctgattctc caggggttac gcagaccata agcccagatt
    10981 tctggcaaac cacccccgcc agcaaacggg ttgtctggcg ggatgccata ggggtcacct
    11041 gagtccacat cgatgcgcag aatctttccg agcaaagttt gagtggactg agcatttccc
    11101 tgtgggtcac cacctgagcc accatcgccc aagcccaggt acaggtagcc atctggtcca
    11161 aagacgacca cgccgccatt atggttgccg aagggctgtg acaccaccag cagtgttttc
    11221 tctgtggttg ggtctgcctg atcaggattc acctcagata ctgtaaagcg ggcgatatgg
    11281 gtatcacctt tagtatcagt gtagtttacg tatagataac cgttctcagt gtagcgcggg
    11341 tggaatgcca gtcccagaag accttgctcg gacccacggt tgccaacccg atcgcgtata
    11401 tccaggaaag gggcatcgag caaagtacca tctttccaga ttaagatgcg tccatcctgc
    11461 tcgacaataa acaaccgacc ggaaccatcc tgagcgttct caagtccgat gggattaacc
    11521 aatccaccca ccacgcgctc ccagagataa tctgcagcct caggcaatac ggcagctgga
    11581 cctgctggag gtgcagtgtc cgctggactt tccgacggga gcgtggttgg aggtgcgggt
    11641 tgtgtcatag actcctggac aggaggttct gccgtcgccg agggttgcac gacttccaat
    11701 ggtattgggt cgcctgggca gaagcaggtg gaacagctgg cgcgtcggta gctggggtca
    11761 taacgctgca cgcagacagc atgaacacca gcaataccca cacattacgc atcatgattt
    11821 ttcttcttca gtgaattccg catcaatgat ctcgccttcg ttggctaatg gatcgcggta
    11881 ttcaccgggt atttcctgcg tgagagctct caggtgttct tgcaccacct ccggcgggca
    11941 gagctcaaca aacaggtagg tccccagcca gataacggca gcatcatcaa gcggacctgg
    12001 agcgatgtcg gggacgatca aataaatcag cgaaccgagg gggagaactt tcaaccaagg
    12061 gcttacgcgc gcgtcggaca tcaagcgcag gatgagctta atgcgaacag ccaaatcctg
    12121 aaaaaagccc ccacctttgg acggggtgat tgagcgattg cgattactgg acataggacc
    12181 tcaaaacact tgccttacac aaaatacttc atcagcccct attcatccct gaaccagata
    12241 ggtaacgaat attgcggata tttcagcgga gaattccgcc gaaacatccg caaactcacc
    12301 ctcctccagc acattgaggg
//
LOCUS       DBNGSX010000048         2275 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1111219, whole genome shotgun sequence.
ACCESSION   DBNGSX010000048 DBNGSX010000000
VERSION     DBNGSX010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1111219"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="AC16CB_01525"
     CDS             <1..721
                     /locus_tag="AC16CB_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3341174.1"
                     /translation="AVEAAHAAHTASPGWAMRHGYNRAQILYFIGENLDARSEEFAEQ
                     IRAMTGRSMKSAQAEVERAVERLFTYAAWADKYGGAVQETTLRGVTVAINEPIGVIGI
                     VCPDEYPLLAFVSLMAPAVARGNTVVVIPSQKYPLSATDFYQVLDTSDVPAGVVNIVT
                     GDRDHLTKTLIEHEDVDSVWYFGSTSGSYHTEYESAANMKRTWVSYGMQRDWLSHEQG
                     EGHDFLHEAIQVKNIWIPTGE"
     gene            complement(853..1392)
                     /locus_tag="AC16CB_01530"
     CDS             complement(853..1392)
                     /locus_tag="AC16CB_01530"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3341175.1"
                     /translation="MINSGFPQSFPVIETERLLLREITYDDTSAIFKNFSDPEIAKWF
                     FEQPLTEIEQTNEFIDQFISDFKQGKGLTWAITLKENGQCIGTCGYGDVEIGDRGEIG
                     FDLAQEHWGKGLMSEGLAAVINYGFSVLKLPRVEAHTYSQNERAKHLLEKLGFQLDHV
                     SEDSHYYLLSKDRKKDPGD"
     gene            complement(1430..>2275)
                     /locus_tag="AC16CB_01535"
     CDS             complement(1430..>2275)
                     /locus_tag="AC16CB_01535"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3341176.1"
                     /translation="TEALGYIMAVFGIGFIIGPALGGLLSAWLGARMPFVIAGLAAAA
                     TALMSRLFLDETVGQKTKNIREFRRVHLTPAEVARNYPLVLILVVAFVGQFGFGMLQS
                     TFALYGSAVLFRGESSQATNLGVGLLLAVVGSGQFITQTWLIGSLKRRFGDPLIVIQG
                     SLIRMTGLAILALVSSPWLAAVSCLFFAVGMGLMMPPLQSLTTRTVSEADRGGVLGLF
                     QSSASLAIILSTAIAGAIFTINPTLPYWIGAILSLAVVLPAWIVLKLSGEGRLKPMEA
                     TNPGD"
BASE COUNT          564 a          620 c          562 g          529 t
ORIGIN      
        1 tgcggtggag gcggctcatg cagcgcatac cgccagccca ggctgggcga tgcgtcacgg
       61 gtataaccgc gcccaaatcc tttacttcat cggggagaac ttagatgccc gcagtgaaga
      121 gtttgccgag cagattcggg ccatgactgg gcgctcgatg aagtcagccc aggctgaggt
      181 cgaaagggcg gtggagcggc tgttcactta cgccgcatgg gcagataagt acggtggtgc
      241 cgtgcaagaa accaccttgc ggggagtcac cgtcgccatc aacgagccga tcggcgtgat
      301 cggcattgtg tgcccagatg aatacccgct gctggccttt gtctcgctca tggctcccgc
      361 ggttgcccgt gggaataccg tggtcgtcat ccccagccag aaatacccgc tcagcgctac
      421 cgacttctat caggtgctgg acacctctga tgtaccagct ggcgttgtca acatcgtcac
      481 cggcgatcga gatcacctta ccaaaaccct tatcgagcat gaagatgtcg attcggtctg
      541 gtattttggc tcaaccagtg ggagttatca cactgagtac gaatcggcag ctaatatgaa
      601 gcgcacctgg gttagttacg ggatgcaacg cgattggctc agccacgaac agggtgaagg
      661 tcacgatttc ctgcacgaag cgatacaggt gaaaaatatc tggattccta cgggcgagtg
      721 agctctggca atagcagact attaaacaca aggcgtttgt aatatcctgg taattgcaga
      781 taatcccttg cagtcgtcgc taccctcgac gactgcaagt ttatgcctag gctattaaat
      841 acataaagcg cttcaatcgc ctggatcttt cttcctgtct ttggatagca gataatagtg
      901 actatcctcc gagacatgat ccaattgaaa gcctaatttt tccaggagat gtttggcgcg
      961 ctcattttga gagtaggtat gcgcctctac tcttggtaac ttcaatacag aaaacccgta
     1021 attgataacg gcggcaagac cttcgctcat taatcctttt ccccaatgct cttgcgccag
     1081 atcaaaccct atctcccctc ggtcaccgat ttctacatcc ccatagccgc atgtcccaat
     1141 acattgaccg ttctctttca atgtgattgc ccaggtgagt cctttgccct gcttgaaatc
     1201 tgagatgaat tgatcgataa attcattcgt ttgctcaatt tccgtcagag gttgttcgaa
     1261 aaaccacttg gcaatttctg gatcggaaaa gttcttgaat attgcgctcg tatcgtcata
     1321 ggttatttct ctaaggagta accgttcggt ttctatgacc ggaaagctct gagggaatcc
     1381 gctattgatc atttccttat tctcctttga cccgccaaac taccccgcac taatctcctg
     1441 ggttggtggc ttccatgggc ttcaatctgc cttcccctga gagtttcagc acaatccatg
     1501 ctgggagcac aaccgccagg gaaaggatgg cgccgatcca atagggcagg gtgggattga
     1561 tagtgaaaat cgctcccgcg atggctgtgc tcaagatgat cgccaggcta gccgaagatt
     1621 ggaataaccc tagcactccg ccgcggtcgg cttcgctcac ggtgcgcgtg gttagggatt
     1681 gcaaaggtgg catcatcaac cccattccaa cggcaaagaa caagcaagag actgcagcca
     1741 gccagggcga actgacaagg gcaaggattg ctaaaccagt catgcgtatc agcgagccct
     1801 ggataactat gagagggtca ccgaatctgc gttttagtga accgatcagc caggtttgcg
     1861 ttatgaattg tcccgagccc accactgcca gcaacaggcc aaccccgaga tttgtagcct
     1921 gactgctttc gcctcggaat aacaccgctg acccatacaa tgcgaaagtt gactgaagca
     1981 taccaaaacc aaactgtccc acaaaagcaa ccaccaggat gagcaccaat ggataattac
     2041 gtgccacttc tgcgggggtc aggtgtaccc ttctaaattc cctaatattc ttcgtctttt
     2101 gacccacggt ttcgtcaaga aaaagcctgc tcatcaaagc ggttgccgcc gcagccaaac
     2161 cagcaatcac aaatggcatc cgcgcgccca accaggccga aagcaagccc cctaaagccg
     2221 ggccaatgat gaagccgata ccgaaaacgg ccatgatata acccagggcc tctgt
//
LOCUS       DBNGSX010000049         6104 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_972627, whole genome shotgun sequence.
ACCESSION   DBNGSX010000049 DBNGSX010000000
VERSION     DBNGSX010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6104)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_972627"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(271..1023)
                     /locus_tag="AC16CB_01540"
     CDS             complement(271..1023)
                     /locus_tag="AC16CB_01540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3341177.1"
                     /translation="MQNKSSSRYDVNIGHADTAMGRYLFEHELSFIQRTLSTTTQPYR
                     MLEVGCGSGQITLALQDAGFPVMGMDIDLAALTICKHQSSTTPLVRGQAPDLPFADSS
                     IDCVIAIECVGFFAHRPFLEESKRVLRDGGLLIFDSVNSRSYRLAMRQLAGRSVTRFL
                     KRLLDRVAGIEQVRHIKTSAGDYQLSSREVLQATSECGFDILAVSGYNWIPFDVRSDS
                     PLVRPAARVEQMLRLEHHYHLSPWFLVAARKV"
     gene            complement(1089..2240)
                     /locus_tag="AC16CB_01545"
     CDS             complement(1089..2240)
                     /locus_tag="AC16CB_01545"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HIR3341178.1"
                     /translation="MIGNQLEHRLNTQETSGYWIWVNEEPEDPDWDAFLAKTPGGHYT
                     QSSLWAKMKALSGWKATRLVVAREDSIIAGAQLLTRPLPLFGKIGYVPKGPLVASDDI
                     HLSQLVINALHRLAKAHGVEYLAVQPPSKGQITHLLPGLGFHANPKMGTVTSTILIDL
                     TRDPNILLAQMRESTRTNIRRAQSRGITIREGTESDLGAFYHLVAVASQRKGYSTYSK
                     KFLAEFWQILDSHGHTKLFFAEYAGEAVSTLLAIHFGDTLFTHVSAWDGRHRELKPNE
                     LLEWETMMWAKAHGYRYYDFEGIDPQVAKVILQGQPLADSHFPVADRMVTRYKLGFGG
                     RVTLLPEVYEYIYSPLLRSVYFQLLPKIEHWPVIAKTWKAVTRAVQPNV"
     gene            complement(2237..3334)
                     /locus_tag="AC16CB_01550"
     CDS             complement(2237..3334)
                     /locus_tag="AC16CB_01550"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HIR3341179.1"
                     /translation="MKVTHITAERREEWNVFVAKEPSFALLQSWGWGEFKEKMGWKVF
                     RIGAEKEGRIVAGVQMLIRPIPPGLTSIAYIPRGPVGDWLNEEVLSLLLTEIHQVARR
                     HRAVFLRIEPPLLNNSTYATVLEKHHFHSSTYTNQPRATITLNLTPAIDEILATMRDK
                     TRYNIRYATKKGVTVRVGNYDDLLTFYRLMRITSLRGGFSHQTLNYYRHEWETFARLG
                     QIKLFVASYQGKTVAANMSAVFGEHGAFLHGASSGEFANLQPNYLLMWEAMLWAKDQN
                     CHTFDLWGIPDEVGLAVYQGQGLPKTDALAGLWGVYRFKRGFSQNVLFYASTYDYIYS
                     APLYKLSENKYVGISIFNRIYTLLDTLKNMR"
     gene            complement(3853..5091)
                     /locus_tag="AC16CB_01555"
     CDS             complement(3853..5091)
                     /locus_tag="AC16CB_01555"
                     /EC_number="6.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HIR3341180.1"
                     /translation="MSSNHKPIKKPYAIVVGLDHMNGIQTARILARHQVPVIAVAKDP
                     QHYCCRTRICERILFTDTATEALINTLEDLGPKLGEKAVLFPCTDAHVLLVSKYRHRL
                     EDWYHVVLPEHDVVEMLMDKSRFYAFAQEKGLPIPRTMFLHNWADAERASQELNFPCI
                     LKPPLSSTPKWEQNSKLKAYLLSNAEEFLTTYKRLAALADMLITQEWVEGPVSNLYSC
                     NCYFNAESEPVATFIARKLRQWPPETGESCLGEECRNDNVLRESLRLFKSVKYRGLGY
                     VEMKQDKRTGKHYIIEPNIGRPTGRSAIAEAGGVDLIYTMYCDALGWPLPPNLVQHYG
                     TVKWIHLRRDFQSAFYYWRRGELTLMDWWRSVRGRKVDALFAWDDPGPFIGDLERAIR
                     LFLSPEERRKRDYSTPLKQT"
     gene            complement(5173..5865)
                     /locus_tag="AC16CB_01560"
     CDS             complement(5173..5865)
                     /locus_tag="AC16CB_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341181.1"
                     /translation="MKLTISPKKITYWLFVIVVALNLFGFMARGIERLLGYNNTEFVR
                     LFDVSEEANITAWFSSLLLLISAALLSVIAMAKSASAKPYSRHWGFLSLIFLYLSLDE
                     AAKIHEITIKPLRALFNASGIFYYAWVIIAIPFILLLGFVFMRFVFDLPSRTRTQFVL
                     AGVIFLTGALGFEMLGGYFLDKLVGGLSINPILVTFEEFFENFGIVIFISALLTYMKS
                     HSDTKDISFELT"
     gene            complement(5862..>6104)
                     /locus_tag="AC16CB_01565"
     CDS             complement(5862..>6104)
                     /locus_tag="AC16CB_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341182.1"
                     /translation="YPKPNLVIFLDAPPEVLYNRKGEASPHYLEKRRETTLEQGKYVP
                     NFVQVDANRPIDQVLSDVTRYIIEFHTNKNSKKTKT"
BASE COUNT         1540 a         1493 c         1477 g         1594 t
ORIGIN      
        1 agacctcgcc actgtgcaag cctatatgat tttcgactta ccattttgga gataaaaggg
       61 gcgtgctaga taaagggata atgaccttgg aaaaatctac atctatctcc gattaataaa
      121 acgaatctaa ggttattttg ttacgggatt acaactcgtc aatttaggtc aaaaaacaaa
      181 aaaccccgca tcaaaccggg gctaaaatgg ctggttatca aaactgtaat gatcacggat
      241 agacgatttt tatcgtaagc ttatttgccg ttaaaccttt ctggctgcca caaggaacca
      301 ggggctgagg tggtagtggt gttcgagtcg cagcatctgt tcgacccgtg ctgcaggacg
      361 caccagcggg ctgtcgctgc gcacatcgaa agggatccag ttgtaaccac tcaccgccag
      421 gatatcgaaa ccacactcgc tcgtagcttg cagcacttct cggctactta gctggtaatc
      481 ccctgctgaa gtttttatat ggcgaacttg ttcaatccct gctacacgat ctaataagcg
      541 cttcagaaat cgcgttacag agcggcctgc gagctgcctc attgccaacc tgtagctgcg
      601 cgagttcacc gagtcgaaga tcagcaggcc gccatcgcgc agcacccgct tactctcctc
      661 aaggaatggt cggtgggcga aaaacccgac gcactctatc gctatcacac agtcaatact
      721 gctatcagcg aagggcaggt ctggcgcttg ccccctgacc aaaggggttg tgctggattg
      781 gtgcttgcat atcgtgaggg ctgccagatc gatatccatc cccatcacag gaaaacctgc
      841 atcctgcagc gccagggtaa tctgcccact cccacagcca acctccagca tacgatatgg
      901 ttgggttgta gtgcttaggg ttcgctggat aaagctcaac tcgtgctcga aaagatagcg
      961 ccccatggca gtatcagcat gtccgatgtt gacgtcgtat cgagaggagg atttattctg
     1021 cataggacct ggatgtgtgg atttgctcat caatttcaat aaccggaatc ttcccggcaa
     1081 acgatcaatt acacatttgg ctggacggca cgcgttacgg ctttccacgt tttggctatt
     1141 accggccagt gctcgatctt cggcaatagc tgaaaataca cactgcgcag taacgggcta
     1201 tagatgtatt cataaacttc gggtaagagt gtcacccgac caccaaatcc aagcttataa
     1261 cgcgtcacca tcctgtctgc aacagggaaa tgtgagtcag ccaggggctg cccttgcagg
     1321 atcaccttgg caacctgtgg gtcaatcccc tcaaagtcgt agtagcgata gccatgagct
     1381 ttagcccaca tcatcgtctc ccactccaac aattcatttg gcttgagctc ccgatggcga
     1441 ccatcccagg cgcttacatg ggtgaacaat gtatctccaa agtggatagc caagagtgtg
     1501 gagacggcct caccagcata ttccgcaaaa aacagcttgg tatgtccatg tgagtcaaga
     1561 atttgccaga attcagcaag aaattttttt gagtaggtag agtatccctt cctttggctc
     1621 gctacagcca ccaagtggta aaaagctccc aaatcgctct ctgtaccctc ccgaatggtg
     1681 atcccacggc tctgggcgcg gcggatgttc gtgcgggtgc tttcgcgcat ctgcgccaat
     1741 aagatgttcg gatcacgggt gagatcgatc aggatggtgg aggtaaccgt gcccattttg
     1801 ggattggcgt gaaatcccaa accaggtaac aggtgggtaa tctgtccttt gctcgggggc
     1861 tgcacggcga ggtactccac accatgcgct tttgctaatc tgtgcagtgc attgatcacc
     1921 agttgggaca agtggatatc atctgaagcg acgagcgggc ctttgggtac atagccgatc
     1981 ttgccaaaaa gggggagtgg gcgtgttaac aattgcgccc cggctatgat tgaatcctcg
     2041 cgtgccacaa cgagacgtgt cgccttccag ccagacaggg ctttcatctt cgcccacagg
     2101 ctgctctgcg tatagtgacc tcctggcgtt ttcgccagaa acgcatccca atcaggatcc
     2161 tcgggctctt cgttcaccca tatccaatag ccggatgtct cttgggtatt taggcgatgt
     2221 tccagttgat ttcctatcat ctcatgttct ttagcgtgtc taacaaagta tatatccgat
     2281 taaagattga gataccgaca tacttattct cgcttagctt atataaaggc gctgaataga
     2341 tgtaatcata cgtgcttgca tagaacaaca cgttctggct aaatccgcgt ttgaagcggt
     2401 acacacccca aagaccagct agtgcgtccg ttttgggcaa gccttgacct tgatatacgg
     2461 ccaagcccac ctcatcaggg atcccccaaa gatcaaacgt atgacagttc tgatcctttg
     2521 cccacagcat tgcttcccac atgagcagat aattaggttg taagttggca aattctccag
     2581 atgaagcccc gtgtagaaat gctccatgct ctccaaatac agcagacata ttggctgcaa
     2641 cagttttacc ttgatatgag gcgacaaata acttgatttg tcctaatctg gcgaaagtct
     2701 cccactcatg ccgataatag ttcaacgttt gatgactgaa cccacctcgg agacttgtga
     2761 ttcgcatgag gcggtagaat gtcagcaggt cgtcgtagtt gcccacccga acagtgaccc
     2821 cctttttggt tgcatagcgg atgttgtagc gggttttgtc tcgcatcgtc gccaggattt
     2881 cgtcaattgc cggtgtcaaa ttcaatgtaa tcgtggcgcg cggttggttt gtgtaggtac
     2941 tgctgtggaa atgatgtttc tctagcactg tggcatatgt agagttgttc agtaacggcg
     3001 gttcgatgcg tagaaataca gcccggtgtc tacgtgcgac ttgatgtatt tcggttaata
     3061 agagggataa tacctcttca tttagccaat cgcctacagg accccggggg atataggcta
     3121 tactggtcaa tccaggtggt atgggcctga tgagcatctg cacgccagct acaattcgcc
     3181 cttctttctc tgctccaatt cgaaaaactt tccaccccat tttttccttg aattcacccc
     3241 acccccaaga ttgtaatagg gcgaaagatg gctccttggc tacaaataca ttccactcct
     3301 ctcgccgttc agcggttatg tgcgtgactt tcattggaat acaacctgca tcacaaaatt
     3361 gcgcctgcaa tagtctgggt cgtgaaagcg aattttaggg tatctggcga aaaaatccat
     3421 aagtaagctt acttggttca gcaatgatgg tttgtgaata tttatatcac gcccgtagtt
     3481 taccagcctg ccccaaattt gggcttaact ctcgcaggtt gtacttatca tcgggtattc
     3541 cgctgccgca aaggaagcct aatgccatgg cttgtggtct tgagcatgtt caaacccttc
     3601 gaacgttcca gggtatgaag aataaccccc tgggtgtgtt taccaacaag ctctttccaa
     3661 atgtggctat ctaagccgct gggaagttcc attgtttaga tgtgaatgga aggccgacgt
     3721 ttaacaaaaa agcttggggg aggccagctc ccccaagatc tcctcccccg cgttatttgg
     3781 gaaataagaa gcagatcctc gctatgggtg cggattttga gacgatatat ccggaatcat
     3841 ttatgtgtga agctacgttt gtttcagcgg tgtgctgtaa tctcttttcc ttcgttcctc
     3901 cggggagagg aataaacgga ttgctctttc tagatcgccg ataaaaggtc ctggatcatc
     3961 ccaggcaaat aaagcgtcta ccttccggcc tcgtaccgac cgccaccaat ccatgagggt
     4021 aagctctccc cggcgccagt agtagaaagc agactgaaag tcccggcgca ggtgaatcca
     4081 ctttacagtc ccataatgtt ggacgaggtt tggtggcagt ggccaaccca acgcatcgca
     4141 atacatggtg tagatcagat caacccctcc ggcttctgca atggctgatc gtccggttgg
     4201 tcgtccaata ttgggctcaa tgatatagtg tttgcctgtg cgcttatcct gcttcatctc
     4261 tacataaccc agcccgcgat attttacact cttgaacaag cgtaaacttt cccgcaatac
     4321 attatcgttg cggcattctt ctcccaggca gctctcgccg gtctctggtg gccactggcg
     4381 aagctttcgg gcgataaagg ttgcaactgg ttctgattcg gcgttaaagt aacaattgca
     4441 cgaatacagg ttgctgactg gcccttcaac ccattcttgg gtgatcaaca tgtcggccag
     4501 cgcagccagg cgcttgtaag tagttaagaa ttcctccgca ttagatagaa gatatgcttt
     4561 taacttgctg ttctgttccc acttaggcgt gctgctgagc gggggtttca gaatacaagg
     4621 gaagttcagc tcttggctcg ctctttcggc gtctgcccag ttgtgcagga acatggtacg
     4681 cggtatggga agccctttct cttgagcaaa ggcgtagaag cggctcttat ccatcagcat
     4741 ctccaccaca tcgtgttcgg ggagaacgac gtgataccag tcttccagcc tgtgccgata
     4801 cttcgaaacc agcaatacat gtgcatctgt gcaggggaaa agcacggcct tttcgcccag
     4861 cttaggtccc aggtcctcta aagtattgat cagtgcttca gtggctgtat cggtgaaaag
     4921 gatccgctcg cagatacgcg tccggcagca ataatgctgc ggatctttgg caacggcaat
     4981 caccggtacc tggtgacggg ctaatatgcg cgctgtctga ataccattca tgtggtcgag
     5041 gccgacaact atggcatagg gttttttgat gggcttgtga tttgatgaca ccaataaaac
     5101 ctccttattc gttgttcgcc cctgtaggga aggagaaggg tcttggtaag cgagagttta
     5161 cagccttccc tatcatgtga gctcgaatga gatatccttc gtatccgaat gcgacttcat
     5221 ataagtcaaa agagctgaaa taaatataac aatgccaaaa ttctcaaaga actcttcaaa
     5281 cgtaacaaga atcggattga tactcagtcc tccaaccagt ttgtccagaa aatatccccc
     5341 aagcatctcg aatcctaacg cgccggtaag gaagatgacc cctgcgagga caaattgcgt
     5401 gcgagttctg gatgggaggt cgaaaacaaa gcgcataaac acgaacccca gtagaaggat
     5461 aaatggtatt gctataatga cccatgcata ataaaaaata ccggaggcgt taaatagggc
     5521 tcttaatggc ttgatggtga tttcatgaat tttggctgct tcatccagag ataagtataa
     5581 aaaaattagg gataagaacc cccagtgtct agaatagggt tttgcggatg cgcttttggc
     5641 catcgcgatg acggataaaa gagcagcgct gatcaacagc aagagggatg aaaaccaggc
     5701 tgtaatattg gcttcttcag aaacatcgaa aagccggacg aattcggtgt tgttataacc
     5761 aagtaatcgc tcaattcctc gcgccatgaa gccaaacaag ttcaaggcga cgacaataac
     5821 aaacaaccaa taagttattt ttttagggga aatagttagt ttcatgtttt cgtctttttg
     5881 gagttcttat ttgtatggaa ttcgataata tagcgagtca catcggaaag aacttgatct
     5941 ataggccggt tagcatccac ttgtacaaag ttcggcacat attttccctg ttctagggta
     6001 gtttctctgc gtttttccag gtaatggggt gatgcttcgc ctttgcgatt gtatagcact
     6061 tccgggggtg cgtccaaaaa aataaccagg tttggttttg gata
//
LOCUS       DBNGSX010000050         5595 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1180712, whole genome shotgun sequence.
ACCESSION   DBNGSX010000050 DBNGSX010000000
VERSION     DBNGSX010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5595)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1180712"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..1595)
                     /locus_tag="AC16CB_01570"
     CDS             complement(168..1595)
                     /locus_tag="AC16CB_01570"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent decarboxylase
                     family protein"
                     /protein_id="HIR3341183.1"
                     /translation="MQIPQNSLSKEEVFATLQRYKSYDMDWKSGKVWAYVYNPGSEVM
                     EVITKAHNLFLAENGLDPSVFPSLLKLETDVVRMVANLLRGDEQVVGHVTTGGTESIM
                     LAVKTARDKARFERPEITAPEMVLPKTAHAAFHKAAHFLGLKPVVTPFDPQTFRADVS
                     AMRSAITENTVLLVASAPCYSQGVIDPIREIGALAQEMGLPFHVDACVGGIHLSFMRE
                     MGYTLPEFDFSVPGVTSISVDMHKYGYAAKGCSTILYRDKSLRRYQIYACTDTTTYSL
                     INPTVLSTKSGGPLAGAWATLNYLGEAGYKRMVSEVQDATHRLIQGINHIEGLRVLGE
                     PDMCMFSFTSDIINVYQLADEMKKRGWYLQGQFSTPLSPRNLHVSVNHGTLPNVDAML
                     RDLGECTEIVKKAKPLDTTFIRSQVARALENPSPETFANLAELAGIHGTDLPNEMATI
                     NEVLDALPDEVANMLLIEYFNELYV"
     gene            complement(1627..3039)
                     /locus_tag="AC16CB_01575"
     CDS             complement(1627..3039)
                     /locus_tag="AC16CB_01575"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HIR3341184.1"
                     /translation="MLAQALDYTRSHQGKSLDRLCEWLRIPSISTLAENAQDVRRAAQ
                     WISEALREMGLDHIEVVETSGHPLVYADWLGAEGPTLLIYGHYDVQPVDPLDEWRTPP
                     FEPQVVGDDLFGRGTTDDKGQAYAALAALEAYLKTSGRLPVNVKVLLEGEEEVLSPSL
                     QVFLRERREKLLADAILICDQEMLGPQTPVIMYGVRGNTYLEVEVRGPAQDLHSGMFG
                     GAGDNPFNVLVRLLAALQDPETRRVTIPGFYDRVRPLSDDERNLIAKIPFDDEIGLHL
                     TGMPALAGEEGYNLAERLSVRPTLDIHGIAGGFTGKGAKTVIPSKATAKLSMRLVPDQ
                     QPDEIARLCEAYLKELAPKTVKLDIRTIGTAMPVVIDFNAPAVRAAEQAYQRSFGYNP
                     VYFRSGGSLPIVHDMIFLLSTPAQGEIPVVMIGFGLPDDNAHAPNEKLHLPNFYKGIE
                     TVIHYLDLLSKSGTIAGDPM"
     gene            complement(3139..4476)
                     /locus_tag="AC16CB_01580"
     CDS             complement(3139..4476)
                     /locus_tag="AC16CB_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3341185.1"
                     /translation="MKRTLFHISYSMGNFANTIAYQVFGNRIQFYYVDILGLNAALAG
                     ILWTIYGVWNAINDPLMGQLSDRTRTRMGRRVPYVFFGAIPLGLSFFFLWTPPTQSPW
                     MLAAYFFALLFIFDTIYSLTMIAYNALFPEVAPTLRDRVDLSATREILATVALLLAFI
                     LAPILAEEVGYIWMGAIMGTFVAIGYLVSMIGVRENLANIKDDSVGIIPSLKIVLSSV
                     PFRWFVGANMAKEYVWLALAAMLPFWRKYALGIQGEGLVFGIRMGAGDQEAILLGLPI
                     VLCIPCLLIWRPIVPRLGNRKTWILASFVFIPGFLVVAFANNFYTGLLGTVLAAPGLA
                     GSMIMPFPVISEVIDDDASRHGYQRSGIFFGMSWGIVKLAFSAQGVLFASVMSAAGYA
                     AGSVGQTASAVSGIRFLIGITPIIACLIVAYCMYRYPLGAAQIKDAPKAEELT"
     gene            complement(4744..>5595)
                     /locus_tag="AC16CB_01585"
     CDS             complement(4744..>5595)
                     /locus_tag="AC16CB_01585"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR3341186.1"
                     /translation="ARKPAMTHHLSVWTIKHALALYFVLAYAFSWAVELPIALSVHGI
                     IPTNVPLASHYFASFGPLLAALVVVSITEGVGGLRRLFQGLLKWRVEAGYALFAIAIP
                     AGLFAVAVVASWIMQGAWPDLGLLNEVDYLPPVGMLPALGLWLLTYGFGEEMGWRGYA
                     LPRLQSGRSAYSAALLLGVLWAGWHIPAIFYRDTYTALGFLALPLLLFSVCIASIIIT
                     WLYNGTRGSLLMVILFHAIFNFFSVSEAGGDSAPIVMTAVIVFWAVRVVKVYGKENLA
                     PVEKQTV"
BASE COUNT         1231 a         1581 c         1635 g         1148 t
ORIGIN      
        1 cattcgaaat acaccaacca tttaggatct gcgctaagta acctaggcct tttcaacgcg
       61 ttcgaaattg gttgtcgttg atgttgtcat gtacactgga tgcatttgat actgatggtt
      121 gatgcaatct ccacatcaag ctcgggattg cgcaacaaaa tatcccctca tacatatagc
      181 tcgttgaaat actcaatcaa cagcatgttg gcgacctcgt ccggcagggc gtccagcacc
      241 tcattaatgg tagccatctc atttggcagg tccgtgccgt ggatgcctgc cagctccgcc
      301 aggttggcga aggtctccgg ggaggggttc tccagcgcgc gggcgacctg cgagcggatg
      361 aaggtggtgt cgagtggctt ggcttttttg acgatctcgg tacactcgcc caggtcacgc
      421 agcatagcgt ccacattggg cagcgtgcca tgattgactg atacatgcaa gttgcgcggc
      481 gagagcggtg tggagaactg cccttgcagg taccagccgc gctttttcat ctcgtcagcc
      541 agctggtaga cattgattat gtccgaagtg aaggagaaca tgcacatgtc tggctctccc
      601 agcacgcgca gcccctcgat gtggttgata ccctggatga ggcggtgggt ggcgtcctgc
      661 acctcgctca ccatgcgctt gtaacccgcc tcccccaggt agttcagggt cgcccaggcg
      721 cccgccagcg gcccgcctga cttggtgctc agcacggtcg ggttgatcag ggagtaagtg
      781 gtggtgtcgg tgcaggcgta gatctggtaa cggcgcaggc tcttatcccg gtacaggatg
      841 gtcgagcagc ccttggcggc gtacccgtat ttgtgcatgt ctaccgagat ggaggtaact
      901 ccgggcacgc tgaaatcgaa ctccggcaga gtgtagccca tctcccgcat gaaagacagg
      961 tggatgccac ccacgcaagc gtccacgtga aatggcagcc ccatctcctg ggccagagcg
     1021 ccgatctcgc ggatggggtc gatcacaccc tgcgagtaac acggtgccga ggccaccagc
     1081 aggacggtat tctcggtaat ggcagatcgc atggcgctca cgtccgcccg gaaagtttgt
     1141 gggtcgaatg gggtcaccac aggctttaag cccaggaagt gagcagcttt gtgaaaagct
     1201 gcatgggctg tcttggggag caccatctcg ggagcagtga tttcagggcg ctcaaagcgg
     1261 gctttatcac gggcagtctt cactgccagc atgatgctct ccgtcccgcc ggtggtgacg
     1321 tgacccacca cctgctcatc gccgcgcagc aggttggcaa ccatgcgcac tacatctgtc
     1381 tccagcttca acaggctcgg gaaaacggat ggatcgaggc cgttctcggc gaggaacagg
     1441 ttgtgggctt tggtgattac ttccataacc tcggagcctg ggttatacac gtatgcccat
     1501 actttgccag atttccagtc catgtcgtag gatttatagc gttgcagcgt agcgaagacc
     1561 tcttctttgg agaggctgtt ttgtgggatt tgcatagttt tctccaggaa tgggggaatc
     1621 aggtatttac atgggatcgc ctgcgattgt cccgcttttc gacaatagat ccaggtaatg
     1681 gatcacggtc tcgatgcctt tgtagaaatt cggcaggtgc agcttctcgt tgggggcgtg
     1741 ggcgttgtca tcgggcaggc cgaagccgat catcaccacc gggatctccc cttgagcagg
     1801 cgtgctcagc aggaagatca tgtcatgcac aatgggcaac gagcctccac tgcgaaagta
     1861 gaccgggttg taaccgaagc tgcgctggta tgcttgctct gcagcgcgca cagctggggc
     1921 attgaaatca atgaccaccg gcatggcggt gccaatcgtg cgaatgtcca gcttaactgt
     1981 ttttggggca agctctttga gataggcttc acacaagcgg gcgatctcat cgggctgctg
     2041 gtcaggaacc aggcgcatgc tgagcttggc ggtggctttc gacggaatga ccgtcttagc
     2101 ccccttgccg gtgaaaccgc ccgcaatgcc gtggatatct agcgttgggc ggacgctgag
     2161 gcgctcagcc aggttatagc cttcttcacc tgccagggcc ggcatgccag tcaggtgaag
     2221 ccctatctcg tcgtcaaagg gtatcttggc gatcaggttg cgttcgtcgt cgctgagtgg
     2281 acgcacgcgg tcatagaagc cggggatggt cacacggcgc gtctcggggt cctgcaatgc
     2341 agccagcaag cggaccagca cgttgaacgg attatcccct gcgccgccaa acataccgga
     2401 gtgcaggtcc tgggctggac cgcgcacctc gacctcgaga taggtattgc cgcgcacgcc
     2461 gtacataatc actggagttt gcgggccgag catctcctgg tcgcagatca agatggcatc
     2521 ggcaagcagc ttctcacggc gctcacgaag aaagacctgc aggctggggc taagcacctc
     2581 ctcttccccc tcaagcagca ccttgacatt caccggcagt cgcccgctgg tttttaggta
     2641 ggcttccagg gcagccaggg cggcataagc ctgaccctta tcgtcagtag taccgcgccc
     2701 gaatagatcg tcgccgacca cttgcggctc gaagggcggc gtgcgccact cgtccaacgg
     2761 gtcaacaggt tgcacgtcat agtgcccgta gatgagcaga gttgggcctt ctgcgcccag
     2821 ccagtcggcg tagacgaggg gatgcccgct tgtctccaca acttcgatgt gatcgagtcc
     2881 catctcgcgc agggcttcgc tgatccattg ggcggcgcgg cgcacatcct gcgcgttttc
     2941 tgccagcgtg ctgatgcttg ggatgcgcag ccattcgcat agcctatcta gggattttcc
     3001 ctgatggctg cgggtgtagt ccagagcttg tgctaacatg atttatcctt cagccaggcg
     3061 agttttgttg ttggattcat ctcgtctcct atttgacctc gatccttaac ctaccgcagg
     3121 ttcagaaact gcggtaggtt aagtaagctc ttcagccttt ggagcgtctt tgatctgggc
     3181 tgctcccaag ggatacctgt acatgcaata ggctacgatc aggcaggcaa tgatcggggt
     3241 gatgccgatc aaaaaacgga tgcctgatac ggcgctggct gtttgcccca cgcttccggc
     3301 ggcatatcct gcagccgaca tcaccgaagc aaagagcact ccctgggcgg aaaaggccag
     3361 cttgacgatg ccccagctca tcccaaagaa gatgcccgac cgctgatagc cgtgacggga
     3421 tgcgtcatcg tcgatcacct ccgatataac cgggaagggc ataatcatcg agcctgccag
     3481 accgggagcc gccagtaccg ttcctaataa accggtgtag aaattattgg caaaggcgac
     3541 cacaagaaac cccgggataa agacaaagct ggcaagtatc caggttttgc ggttgcccag
     3601 gcggggcacg atcggacgcc agattaacag gcaggggatg cacagcacaa tcggcagccc
     3661 cagcaagatg gcttcctggt cgccagcgcc catgcgaatg ccgaatacca gcccctcacc
     3721 ctggatgccc agggcgtact tgcgccagaa gggtaacata gctgccagcg ccagccagac
     3781 gtattccttt gccatattgg cgcccacgaa ccagcggaaa ggcacgctgg atagcacgat
     3841 cttcagggat gggataatcc caaccgagtc atccttgatg ttggcgaggt tttctctcac
     3901 cccgatcatg gagactaagt agccaatcgc gacgaaggtg cccatgatgg cgcccatcca
     3961 gatgtagccc acctcttccg ctaggatggg cgccaggatg aaggctagca gtaaggcgac
     4021 agtcgccaga atctcgcgcg tcgccgacag gtccacccga tctctcagcg tgggagccac
     4081 ctcggggaat aaggcattgt atgcgatcat ggtcaggctg tagatggtat cgaagatgaa
     4141 cagcagggcg aaaaagtatg ctgccagcat ccacgggctt tgggtgggag gcgtccacag
     4201 gaagaaaaat gacagaccga gaggtatggc accgaagaag acgtaaggca cgcgccgccc
     4261 catgcgggtg cgcgtccggt cggagagctg ccccatcagc gggtcgttga tggcattcca
     4321 cacgccatag atggtccata ggatgcccgc caatgctgca ttcaggccca ggatatccac
     4381 atagtagaac tggatgcggt tgccaaacac ctggtaggca atagtgttgg cgaagttccc
     4441 catggagtaa ctgatgtgga aaagcgtcct cttcatgatg cgcttgcctc ccggctttgg
     4501 tcttggtgga tccgccttgc tgccccaccc acatcgtacc cattataaaa agttaaaccc
     4561 ctttgcccta tatgtggtta cggtattgac aatcttaatg ccaagtgtag ctcgttgaag
     4621 cgcgcataaa actcgcccgc gctatccgtc tccaatcgag gacggcagcc tgaaatcccc
     4681 ccggtgatac acgtcgtttt gcaacgggcg ataggtacta ctcgaatgtc aagtacgctc
     4741 ggactaaacg gtttgctttt ctactggcgc caggttttcc tttccgtaca ccttgaccac
     4801 ccgcactgcc cagaagacga tcaccgccgt catgacgatt ggtgcgctat ccccacccgc
     4861 ttctgagacg gagaagaaat tgaagatggc gtggaacagg atgaccatca acaggctccc
     4921 ccgggtgccg ttatacaacc aggtgataat gatcgaagcg atgcagacag aaaagagcag
     4981 cagcggcaac gccagaaaac ccagggccgt gtaggtatct cgatagaaga tggcggggat
     5041 atgccagcct gcccagagga cacccaaaag taaggcagcg gagtaggctg accgccccga
     5101 ctgcaggcgc ggcagcgcat aaccgcgcca acccatctcc tcgccaaagc catacgtcag
     5161 caaccataga ccgagcgctg ggagcatacc cacagggggt aggtaatcaa cttcattgag
     5221 cagcccgaga tcaggccagg cgccctgcat gatccatgaa gcgacgactg ccacggcaaa
     5281 aagccccgcc gggatggcga tcgcaaacaa ggcgtagccc gcctcaacgc gccatttgag
     5341 caacccctgg aatagcctgc gtaggccgcc cactccttct gtgatgctca ccacaaccag
     5401 ggcagccagg agcggcccga aagaagcgaa atagtggctg gcgagcggca cgttggtagg
     5461 gatgatgccg tgtacggaga gggcgatggg gagttctaca gcccatgaga aagcgtatgc
     5521 cagcacgaag tacaaggcta aggcgtgttt aatggtccaa acggaaagat ggtgtgtcat
     5581 cgcaggcttc ctcgc
//
LOCUS       DBNGSX010000051         3393 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_557096, whole genome shotgun sequence.
ACCESSION   DBNGSX010000051 DBNGSX010000000
VERSION     DBNGSX010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_557096"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..510
                     /locus_tag="AC16CB_01590"
     CDS             19..510
                     /locus_tag="AC16CB_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341187.1"
                     /translation="MQTKKIVLTTIAILLALSVIAASVLPATTSTIKKGETVEFYSGR
                     AGVSFTDSQFTGAVRLARIDDSKIKATNHPKFKHDLLSVRFTKSNGEKVTHVLGAVYV
                     FFIVNRSEVSAWEKGELAVYVYDSWKNEWQECYSFLVNDSDGKSSLACRMRIFSTYGV
                     AKK"
     gene            complement(532..2025)
                     /locus_tag="AC16CB_01595"
     CDS             complement(532..2025)
                     /locus_tag="AC16CB_01595"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3341188.1"
                     /translation="MRFFLPHTIQARLILSHLLVSLASVALIASYAGQILYSAVRQQA
                     EHRYEVLSFAAARDVEGSLIDYLSGQSSNERVRQSLARIFSSQPEVHYTIYLPDGIPL
                     MDSSGNLPPAADPQTRPELWEALSRQTGEGEVFRRSWQGEEFLYLVTRIEREDQVYGL
                     LSINIPLEAVLKPARSSLLLLIGFALIIGLVVSVAGFFLARSLAEPIEQITHAAESLT
                     SDKMSARFDSSTNLQEINLLAVAFNNMAARLESYVNELRSFVANASHELRTPLTSIKL
                     RVEALRNGALDDPPVSERFLGEVESEVDRMSSMVNDLLDLSRIETGLDPSRHEPVDLA
                     TITYDVFDAFKVRAERAEIQLGSSIHPDLPPVMGNEEQLRRMLYNLVDNSVKYTARNG
                     RVEISLEVNEQGGNLLLKVSDTGYGIAPVHLPHLFERFYRVEATRPRYGSSTGSGLGL
                     PIAKSIIEAHGGKIGVDSQLGQGTVFWVELSTLKMPISRANMNGTGE"
     gene            complement(2026..2724)
                     /locus_tag="AC16CB_01600"
     CDS             complement(2026..2724)
                     /locus_tag="AC16CB_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3341189.1"
                     /translation="MTRIMLVDDEPLITDSLTYSLRREGFDVKSVGDGSIALQEILDY
                     APDLVVLDVMLPGMNGLEICRRLRAQSTIPVIMLTARGEEIDRVLGLEVGADDYLAKP
                     FSFRELLARVRSILRRVELDRQVSMMQSINLGNLTLDSTARRVFKSGQEVQLSAREFD
                     LLSCLMKNAGRALSREDLLGEVWGSDWIGDQRTLDVHVRWLRLKVEDDPASPKYIQTV
                     RGHGYRFAGPEELE"
     gene            complement(3126..>3393)
                     /locus_tag="AC16CB_01605"
     CDS             complement(3126..>3393)
                     /locus_tag="AC16CB_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341190.1"
                     /translation="IIDLVTKQAEPRFRVIEQGKCRLCRVEIFSLVIGMASKTTPDVS
                     YQAVRPILAMDLPGHAAVALQAQGVLRGFERLVTTAAIRLDFGV"
BASE COUNT          857 a          931 c          845 g          760 t
ORIGIN      
        1 taagcagagg agggtgccat gcaaaccaaa aaaatcgtct taaccacaat tgccatcctt
       61 ttggcgttat ccgtcatcgc ggccagcgtt ttgcccgcta cgaccagcac catcaagaaa
      121 ggcgagacgg tggaattcta cagcggacgg gcaggcgtca gcttcaccga tagccagttc
      181 accggggctg tgcgcctggc gcgcattgac gacagcaaga tcaaagccac aaaccacccc
      241 aaattcaagc acgatttgct tagcgtgcgt tttaccaaat ccaacgggga gaaagtcacg
      301 cacgtgctgg gcgcggtgta cgttttcttc attgtaaacc gctctgaggt aagcgcctgg
      361 gagaagggcg agctggctgt gtacgtctac gatagctgga agaatgaatg gcaggaatgc
      421 tattcattcc tggtcaatga ctccgatggt aaatctagcc tggcctgccg aatgcgcatt
      481 ttcagcacgt acggtgtagc caagaagtaa tcagcagtct gctgatattt cctattctcc
      541 cgttccgttc atatttgcgc gggaaatagg catttttagc gtggatagct caacccaaaa
      601 aactgtgcct tgccccaatt ggctgtctac gccgatctta ccgccgtgcg cctcaataat
      661 agatttcgca attggcagac caagaccgct tcccgtggat gagccatagc gagggcgcgt
      721 agcctcgacg cggtagaaac gctcaaatag gtgggggaga tgcaccggtg caatcccata
      781 gcctgtgtca ctgactttca gtagaagatt tcctccttgc tcatttactt ccaaggaaat
      841 ctctacccgc ccattgcgag cagtatactt cacggaattg tccaccaggt tatacagcat
      901 acgccgcaac tgctcctcgt tgcccatcac gggaggcaga tctggatgga tggaagatcc
      961 tagctgaatc tcggctcgct cagcccgcac cttgaaggca tcaaagacat cgtaggtgat
     1021 agtagccaaa tcaactggct catgccggct cgggtccagc ccggtttcga tgcgcgacag
     1081 atccaataga tcgttgacca tactgctcat gcgatccacc tcgctttcga cttcacctag
     1141 aaagcgttca gataccgggg gatcatccaa agcaccgttg cgcagagctt cgacacgcaa
     1201 cttaattgag gtcagcggtg tgcgcaactc atggctggcg ttcgccacga agctgcgcaa
     1261 ctcgttcacg taggactcaa gccgggccgc catattattg aatgccaccg ccaaaaggtt
     1321 aatttcctgt agattggtcg atgagtcaaa gcgcgcgctc atcttatcgc tggtcaggct
     1381 ctcggcggca tgcgtgattt gctcaatcgg ctctgccaga ctgcgcgcca ggaaaaagcc
     1441 agctacgctc accaccagcc caatgatcaa agcaaaccca atcagcaata acaaggagga
     1501 gcgcgctggt ttcaggacgg cttccaaggg aatattgatg cttaatagcc catacacttg
     1561 atcctcgcgc tcgatgcgcg tgacgagata aaggaactct tcgccttgcc atgaccgacg
     1621 aaatacttcc ccttcccctg tttgcctaga taaggcttcc caaagctcag gcctggtttg
     1681 cggatcagcg gcagggggta aattgcctga actatccatc agagggattc catcaggcag
     1741 gtaaatcgtg taatggactt ccggttggct ggaaaaaatc cgggctagcg attggcgaac
     1801 tcgttcattt gacgactggc cagacagata atcgatcaaa gacccctcga catctctggc
     1861 agctgcaaaa ctcagaactt cataacggtg ttcggcttgc tggcggacgg cagagtatag
     1921 aatttggcca gcataagaag cgatcagggc cacgctggca agtgaaacca gcagatggga
     1981 aaggatcaac cgcgcctgga tcgtatgagg cagaaaaaag cgcatttatt ccaattcttc
     2041 tggcccagca aatcgatagc cgtgaccacg cacggtctgt atatatttag ggctggcagg
     2101 atcatcttcg acttttaagc gcagccaacg cacatgcaca tccagggtgc gttggtcgcc
     2161 aatccagtca ctcccccaca cctctccaag caaatcctcg cggctcaggg cacgaccggc
     2221 gtttttcatc aagcaagaaa gcaaatcaaa ctcacgcgcg gatagttgca cttcctggcc
     2281 gctcttaaac actcgtcgcg ccgtagaatc tagagtcagg ttacctaagt taatcgattg
     2341 catcattgat acctgacgat ccagctccac acggcgcaaa atcgagcgca cgcgagccag
     2401 gagctcccgg aatgagaacg gcttcgccag ataatcgtct gcccccactt ccagacccaa
     2461 tacgcggtcg atctcctcgc cacgcgcggt gagcataatc acagggatgg tactttgggc
     2521 acgcaatcgg cggcagattt ctaggccgtt catgccaggc agcatcacat ccagcaccac
     2581 cagatccggc gcataatcca ggatttcctg tagagctatg gagccatctc ccactgattt
     2641 gacatcaaac ccctcgcggc gcaagctata ggtgagggaa tcggtaatga gaggttcatc
     2701 gtcaacaagc ataatgcgcg tcaaggtcca cctccaaatc cagtttttat tcgaaactct
     2761 cgatcgcaca caaagataat caatgtttta agaaaatatg attaagttct gtttaaggtt
     2821 ttccaaatag ccgtcacgct ttcgcccaaa tggcgacaat ataggttagg aaagtcggcg
     2881 attgggcaat ttagctttca atattcaagg ctaaattatg caaatcttat cacattctgt
     2941 gagaaaaacc ggcaaattac ctcatgagga gagtgcaccc gttttcatcc atctttggat
     3001 tcctggccac tgctctttta ttggccggtc tggctatgct acttgtgcgg gacggaggga
     3061 ttgccttcag tccgggcaaa ttgagtgcga aaagccagcc cggcatttcg ctgcagggtt
     3121 ttacttcaca cgccgaaatc gagccggatt gcagccgttg tcaccagccg ctcaaaacca
     3181 cgcaggacac cctgtgcctg gagtgccaca gcagcgtggc cgggcaaatc catcgcaagg
     3241 atggggcgca cagcttgata gctgacatca ggcgttgttt tgcttgccat accgatcacc
     3301 aaggagaaga tttcgaccct acacaagcgg catttgcctt gttcgatcac tcgaaaacgc
     3361 ggttcagcct gcttcgtcac caggtcaatt atg
//
LOCUS       DBNGSX010000052        11376 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_972701, whole genome shotgun sequence.
ACCESSION   DBNGSX010000052 DBNGSX010000000
VERSION     DBNGSX010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11376)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11376)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11376
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_972701"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="AC16CB_01610"
     CDS             complement(<1..578)
                     /locus_tag="AC16CB_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sulfuration enzyme family protein"
                     /protein_id="HIR3341191.1"
                     /translation="MHIETQCIHAGQHPDPATGAVMTPIYQTSTYVQDRIGLHRGYEY
                     SRTGNPTRTALEACLTDLEGGQYGLAFSSGMAATDAVLRLLSPGDHVVASDDVYGGTY
                     RLFEQVLRPFGLDFTYVDTSNLAQIRAALRPQTRLVWLETPTNPLLSVSDVHAIVEIC
                     QAHPSLPLLVVDNTFATPFLQRPLELGADIVV"
     gene            823..1209
                     /locus_tag="AC16CB_01615"
     CDS             823..1209
                     /locus_tag="AC16CB_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341192.1"
                     /translation="MDIFFQDPTAIPLPPNEVRIKELLAEPWPDNRRVRITLEVTPFQ
                     KRPNGQIAITNPLGDEVASVSIIESITPRMEITMHLRGETPAGEYRVSAAIFYVNEPG
                     DQDAPHEERESVVVDQAEITFQISQM"
     gene            1354..3234
                     /locus_tag="AC16CB_01620"
     CDS             1354..3234
                     /locus_tag="AC16CB_01620"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3341193.1"
                     /translation="MQQDYMLKEAIEAVREGYKVRARDLLTRLLRADQTNPVYWLWMS
                     SVVETSREQVYCLNNVLRLDPHNEAARRGLTLIGAMPADQDMHPTPLVRRQWNVAVQD
                     EPPQGIKALWANPVIRISFSGTLALVVIALIFVGIFGLGEKPIAVAARPTKTPGPPPT
                     FTSTPTYIGSTRTANKITPTPPVTGPLPLWMLLDATYTPTPVYVNTPHPISEDYRIGQ
                     RAFGRGEWGVALRHFRNASQVQPDAADIQYLIGEAQRMVGDYRAAINSYDVALSMNQG
                     FAPAYLGRALASQAIDPKRDVGDDLAKAVQYDPDFGLARLEKASYALAGGDFEAAQED
                     LEVAKQIMTDSPLLAFYLAELALQSGDRVAALEYARQAYELDRTLLPAYRLLGEMALA
                     NGEFDTAREVLEIYVQYAEEDARGWLAMGQAYMEFAGPEQAYRDLVQKARKRDVEAAM
                     QAFERAIELDKTLPGVYLYRAVTHLAMNEGQPAVNDLMMARRLDTNSFAINLGLGRAL
                     LVAERLDDARAQIDSSEKLAENDAQLAAIYYWRALAMEAGNLERAAAPVWEAMLNLAD
                     DSVPEEWRLVAMAHIAALTPTPTVTFTPTSSSTPTATPTRKPTLVGTSLPTSKVTSTP
                     TQ"
     gene            3502..6153
                     /locus_tag="AC16CB_01625"
     CDS             3502..6153
                     /locus_tag="AC16CB_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3341194.1"
                     /translation="MSNEADLSHVITLGGGLLYGPVVACWAFTIGVALGYGIRWFESR
                     KWNQSSIPPAVPVLDVLTLIGVHHIAMLVALPLFGLWQVVSPIPPSSAVIWGEMGPAL
                     LLFALIYMFLYIGNTLVAGKKQYDFWRDLLYLALLKLLPLPFVLVVVMGYPALGLGAL
                     VFLGTIPVVMEVLFYGTYSIRRDLDRRSQELHTLNHISRTLRTAVDLNQLLEEVLNQV
                     SQLFAVENFYIALYNPEEEQLWYPIAVKHFQRVNWPPRALEDRLTDRVIRERSAILLP
                     HNARDALASVGLPAAEDSPTAWMGVPLISSERVIGCLGVFSFSPEVVFSKADVRWLTT
                     LSGQVSVAIENMLRYQTAQRRADLALNQQVHQLTILEAVGRELAAAIRSDHLMDVILG
                     YALEVTHSKWGSISLYEPIENLMAIKALRGYQKAIVSLSAEHGLCGRAVTTGQKIYAA
                     DVLQEPDYVDLTAGEARSQLCVPLLHESRVLGVLTLESEQVDGFSESDQAFINQLATQ
                     ATVALVNAELYAQAQERISEFSAVFNSVGEGLLVLDSSGQITLANEAIRILTGLSPTE
                     FSGQRLVDLPETILAYLGYNSEQAEALVGAMGQGQVLASPRATIRISELNSERILERT
                     SFPVWDRRGRVTGWMIVLRNMTEEIQITQAKDLITETLVHDLRSPASAVLGALDVLKD
                     SLNHDQTENEIVVQAIQVARRGAQRMLGMTESLLEISRLQSGKIETNLLNFSLRPMAT
                     SVLNDFKTDALEYGIILHNDIPDDLSAVRADLNKTTRVLTNLVDNALKFTPAGGQISL
                     SAAHLTDGFITVKVSDTGPGVPEGYREKIFERFSQVPGLVGRRRGSGLGLTFCRLAIE
                     AQGGKIWMEPRPGGGSIFMYTLPVSRA"
     gene            6326..6625
                     /locus_tag="AC16CB_01630"
     CDS             6326..6625
                     /locus_tag="AC16CB_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR3341195.1"
                     /translation="MPVYTYRCENCGVQFDRHQSFSDLPLTRCPECSKKTLRKVYTPV
                     GIVFKGSGFYATDNRSPSGSSRSSTGSEKMSEGKKDKKESESSSSTNSDGKEKSA"
     gene            6718..8055
                     /locus_tag="AC16CB_01635"
     CDS             6718..8055
                     /locus_tag="AC16CB_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HIR3341196.1"
                     /translation="MKNEEMLARIREDQVKFISLQFTDVTGTVKSVDIPSGRLESALE
                     DGVWFDGSSVEGFARIQESDMRLVIDPSTYAVLPWTPQELRRARVFCDIYLPNGNPFP
                     GDPRGTLKRILAEIESRGWLFNVGPEPEFFLFRRNGEKPIHPVPHDVGGYFDFSANDD
                     AVRVRTELMNALSGMGLDVEMGHHEVALGQHEIDFRYADALRAADNILTLKYTVKAIA
                     AQHGLVASFMPKPIFGINGSGMHCHQSLFNKEGENLFFDANDEYNLTKLAYGFIAGQL
                     GHARALAAVVSPTINSYKRLVPGYEAPVYVGWAQINRSALIRIPRYTPGREKSSRAEL
                     RFPDPSANPYLALTVMLAAALDGIDNNLPCPPPLNNVNVYHLTPEERVAMNIPELPGS
                     LAEALRELENDKVLQAALGAEAYEAFYRAKWAEVEEYRMRVTDWEVERYLETA"
     gene            complement(8137..9096)
                     /locus_tag="AC16CB_01640"
     CDS             complement(8137..9096)
                     /locus_tag="AC16CB_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP-loop family protein"
                     /protein_id="HIR3341197.1"
                     /translation="MKCRKCEAKAIINMRQHKLALCKQHFPIWIAEQTQRFIEKYHMF
                     SQEDRLLVAVSGGKDSLSLWDILHQLGYKADGLYIGLGIDGGLHYSDESHRLANKFAI
                     ERNLHLRVVDVHETYGETIPEIAERTTRGKGRPCSVCGLTKRHVMNRIARDEGYDVLV
                     TGHNLDDEAAVLFANTLNWSPGYLMRQSPVLQARQGMVRKAKPLCRFYEREMAAYALV
                     RGIDYIYDECPYVEGSKAIYYKEILNKLEADRPGAKLSFYLSFLQAKESGLFTSPPET
                     LAEIGASGEILNSCPSCGQPTTAPGECAFCRMVIQVSAVNPTG"
     gene            complement(9145..9393)
                     /locus_tag="AC16CB_01645"
     CDS             complement(9145..9393)
                     /locus_tag="AC16CB_01645"
                     /inference="COORDINATES: protein motif:HMM:NF014636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR3341198.1"
                     /translation="MRGRERQTIAMTAKLILRDKEYIVKSGTTVRHAIEKIGVNSETV
                     LATRAGELITDDEILLEDDVIKLVAVISGGRTTEDVDG"
     gene            complement(9380..10231)
                     /locus_tag="AC16CB_01650"
     CDS             complement(9380..10231)
                     /locus_tag="AC16CB_01650"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3341199.1"
                     /translation="MNEFHANDISLGRGQPVILIHGMAASLYDWTEIAPSLAANGYSA
                     YALDLLGHGDSAKPNDPQVYQVEALYDHFREWVNSHDIDSPPILVGHSLGGYLSLVHS
                     LRHPEQVRGLVLINPYYKAQQLSLLLRLVRRRPALSEKAMRVTPHWMIRAVMGWNPET
                     MTNFSAKARQQIVADYKRASPHFVYITHEMPDLSDQLAQVSLPVLVIWGKRDQTLHPT
                     SFPQLVQALPNAFGYPIQSAGHQPHISSPELVTRVMLEFIRKLNSSSSTNQPSPHQWS
                     EGHFAWS"
     gene            complement(10273..>11376)
                     /locus_tag="AC16CB_01655"
     CDS             complement(10273..>11376)
                     /locus_tag="AC16CB_01655"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3341200.1"
                     /translation="PEVPITPNDLAVIIYTGGTTDVPKGVMLSHQNLVANALQTRHWI
                     PDASEGRERFLCALPFSHSYGLTTALNVPIALGATLILKPRFEINDVLKTIKQYRPTI
                     FPGVPQMYVAIKDYPGVRRYGVSSIKACISGSAPLPVEVQEAFEKLTRGRLVEGYGLT
                     EASPVTHGNPLKGLRKVGSIGVPFPSTEARVVDLRNGKKEVRVGQIGELAVRGPQVML
                     GYWQNEEETRRVLTTDGWLLTGDVAQMDSDGYFRIIARKAEMWYPSKPGEPAFPRDVE
                     EVLFEVPQVKEAAVVAVAGQPIAFIIARSERPTADALVAYCKRRLPPEIVPRLVIFVD
                     DFPRSFIGKVLRRELAKRFEERTKEEGEQTIDE"
BASE COUNT         2689 a         2827 c         2987 g         2873 t
ORIGIN      
        1 tgcactacaa tatcagcgcc tagctccagt ggcctttgta agaaaggcgt ggcgaaggta
       61 ttatcaacga ccaagagcgg aagggatgga tgcgcctggc atatctctac gatggcgtgt
      121 acatcgctta cgctgagcag cgggttggtg ggtgtctcca gccaaaccag gcgggtctgt
      181 ggacgtaaag cggctctaat ttgagccagg ttggatgtgt ccacgtaggt gaaatccagc
      241 ccaaagggac gtaaaacttg ctcaaacaac cggtaggtac cgccataaac atcatcgctc
      301 gccaccacat ggtcacccgg agagagcagg cgcagcacag cgtccgtagc tgccataccc
      361 gaggaaaatg ccagcccgta ttggccgcct tccaaatctg taaggcaagc ttccagcgcc
      421 gtgcgcgtgg ggttgcccgt gcgtgaatac tcgtagccgc ggtgtaatcc tatcctatcc
      481 tgaacatagg ttgacgtctg ataaatgggc gtcataacgg ctcccgtagc agggtcaggg
      541 tgctgcccgg cgtgaatgca ttgcgtttcg atatgcatgg attgatcctc cttcgcgaac
      601 atcattgtag ccaaggttgg gctagagggg aagtgaatta atgagatatt ttcaaaccct
      661 cgtagctctc taaggtaagc cttctctgaa gacgggtgac aataaaactg tctgcccttt
      721 tacatgatta ggggagaagt ttgattcttt ggtttgtgtt cgctagcaga ccgcctctgg
      781 caagggttat aatcatctgg aaaaattacc aaaggaccca tcatggatat tttctttcaa
      841 gatcccacgg caattccctt gccgcccaac gaagtacgta taaaagagtt gctcgcggag
      901 ccctggccag ataatcgtcg ggtgcgaata accctggaag tgactccctt tcaaaaacgc
      961 cccaatgggc aaatagctat taccaatcca cttggagatg aggtcgccag cgtcagcatc
     1021 attgagagca ttacccctcg catggagatc accatgcacc tgcgtgggga aaccccagcc
     1081 ggggaatacc gtgtttcggc agcgatcttc tatgtcaatg aaccgggtga tcaagatgct
     1141 ccccacgaag agcgagaaag cgtcgtagtc gatcaagcgg agatcacatt ccaaatctca
     1201 caaatgtagt acatttgggg actcattcat atcatctcct tgcattggtt atcagcccat
     1261 tgagaatcct ttcaaacttg tattgtcctt atagcccctg ccgtccttgt ctgcatagcg
     1321 agcctatgct aaactccttt gtgggaacaa gccatgcagc aagattacat gctcaaagag
     1381 gctatcgaag ccgtccgcga ggggtacaaa gtccgggcgc gggatttgct tactcgcctg
     1441 ctgcgagctg accagacaaa cccggtatac tggctgtgga tgagcagcgt ggtggagaca
     1501 tcacgcgagc aagtttattg ccttaataac gtcctgcgcc ttgacccaca caacgaagcc
     1561 gctcggcgag gattaaccct gattggcgcc atgccagctg atcaggatat gcacccaaca
     1621 ccgctggtgc gtcgccagtg gaatgtggct gtccaggatg aaccgccaca aggcatcaaa
     1681 gccttgtggg ctaaccccgt aatacgaatc tcgttttctg gcaccctagc acttgtggtt
     1741 attgccttga tttttgtagg tatttttggc ttgggagaga aaccaattgc agttgctgcc
     1801 cgccccacga aaaccccagg tccgcctccc accttcactt ctacaccaac ctatattggc
     1861 agcacgcgta ccgcaaacaa gattacccca acaccaccag tgactggacc gctcccgctt
     1921 tggatgctgc tcgatgcgac ctacacgccc acgccagtct acgtaaatac gccacaccca
     1981 atcagcgagg attaccgcat tggacagcgt gccttcgggc ggggtgagtg gggggtagcc
     2041 ctgcgtcatt ttcgaaatgc gagccaggtg caacctgatg cagcagatat tcaatacttg
     2101 atcggtgaag cacagcgcat ggttggagat taccgcgctg cgattaattc ctacgatgtt
     2161 gcactaagta tgaatcaagg ttttgcgcca gcctaccttg ggcgggcgct tgccagccag
     2221 gcgatagatc ccaaaaggga tgtgggcgat gacctggcaa aagcggtgca atatgatcct
     2281 gattttggtc ttgctcgcct ggagaaggcc tcttatgctc tggcgggggg agattttgag
     2341 gctgcacagg aagacctgga agtagctaaa cagatcatga ccgattcacc cttgctggca
     2401 ttttacctcg ctgagcttgc ccttcaatct ggtgaccgcg tcgccgcgct agaatatgcc
     2461 cgccaagcat atgaattaga ccgcaccctg ctacctgcgt accgattgtt gggagaaatg
     2521 gctttagcca atggtgagtt tgacacggcg agggaagtgc tagaaattta tgtccaatat
     2581 gctgaggagg atgcgcgcgg gtggctggca atggggcagg catacatgga atttgccgga
     2641 cctgagcaag cgtatcgtga cttggttcag aaggcgcgaa agagggatgt tgaggcagca
     2701 atgcaggctt ttgagcgtgc cattgagttg gataaaactt tacctggtgt gtacttgtac
     2761 cgcgcagtta ctcaccttgc tatgaatgaa ggtcaaccag cggtcaacga cctgatgatg
     2821 gcccgccgcc tggataccaa ctcattcgcc atcaacctcg gtttggggcg tgccctcttg
     2881 gttgcagaac ggctggatga tgctcgcgcc caaattgaca gttctgaaaa actggcggag
     2941 aatgacgcac agctcgcggc tatttattac tggcgtgcat tggcaatgga agctgggaat
     3001 ctggagagag ctgcagctcc ggtttgggaa gcgatgttaa atttagcgga tgactcagta
     3061 cccgaagagt ggcgactagt cgccatggcg catattgctg ccttgacccc aactccaacg
     3121 gtgactttca ctcccacatc ctcttccaca ccaacagcca cgccgacacg aaaacccacc
     3181 cttgttggca cttctttgcc aacaagcaag gtgacgtcaa cccctaccca ataaacgaat
     3241 gttctgtttt atcaactttc tgtgaatctc atggaagacc gttcggacct gattacacta
     3301 ctcagtcaaa agtcgcctca gaggccaaaa atcattcggg atattaatca accggtttgg
     3361 tttcgctggc ttgtatcctt tgttggcttc cttcttttgt tagtaagtac ttggtttgca
     3421 accacagtgc aagatcattt ttggtacata ttgttaatcg gtttatttgt agcttttcaa
     3481 gtcattttcc ccctgaaatt attgtctaat gaagctgatc taagccatgt gattacattg
     3541 ggtgggggtt tattatatgg accggtggtg gcttgttggg catttacgat cggcgtagcc
     3601 ctgggatatg gcattcgctg gtttgaatca agaaaatgga accaatcttc aattccaccg
     3661 gcagttcccg tattggatgt tttgacgctg atcggagttc accatattgc catgctcgtg
     3721 gctttaccgt tgtttggttt gtggcaggtt gtcagcccaa ttccgccttc atctgcagta
     3781 atttggggcg agatgggccc ggcgttactt ttattcgcac tgatatacat gttcctttac
     3841 atcggaaata cactggttgc gggcaagaaa caatatgatt tttggcgcga tttgctttat
     3901 cttgccttgt tgaagttgct gcctttgcct tttgtgttgg ttgtggtaat gggttatcca
     3961 gcacttggat taggtgcgct ggtgttttta gggactattc ctgtagtaat ggaagtgctt
     4021 ttctacggaa cctacagcat tcgcagggat cttgatcggc gttctcaaga gttgcatacc
     4081 ctaaatcata ttagccggac cctgcgaacc gccgtcgact tgaaccaatt attagaagag
     4141 gtcctaaatc aggtcagcca gctatttgcg gtggaaaatt tttatatcgc attatataac
     4201 cccgaggaag agcaattgtg gtatcccata gccgttaaac attttcagcg agtaaactgg
     4261 ccaccgcgtg cgttagaaga tcgtcttaca gatcgtgtta ttcgcgagcg gagcgctatc
     4321 ttattgcctc acaatgcgcg tgacgctcta gcgtctgtag gtctgccggc tgctgaagat
     4381 tctccgactg cctggatggg agtgccattg atttcgtctg agcgtgttat tggatgcctg
     4441 ggtgtattct ctttctctcc tgaggtggtt tttagcaagg cggatgtgcg ttggttgacg
     4501 actttatcgg gacaggtaag tgtcgccatt gaaaatatgc ttcgttacca gactgctcag
     4561 cgacgtgcag atttggcttt gaatcagcag gtacaccaac ttactatcct tgaggccgta
     4621 ggccgagagc tggcggcagc catccggtcg gaccacttga tggatgtcat tcttggctat
     4681 gccttggagg taacccactc gaaatggggg agcatctctc tctatgagcc catcgaaaac
     4741 ctgatggcga ttaaggctct gcgtggatat cagaaagcaa tcgtttcatt gtccgcagag
     4801 catggtctct gcggtcgggc tgtaactacc ggacaaaaga tctacgcagc ggatgtcttg
     4861 caggaaccag actatgtcga cttgacggca ggggaagcca gatcacaact ttgtgtgcca
     4921 ttgctgcatg aaagccgcgt tctgggggtg ttgactctag agagtgagca agtcgatggc
     4981 ttttcggaaa gcgaccaggc tttcatcaac cagttagcta cccaggcaac ggtagcgtta
     5041 gttaacgctg aactttacgc ccaagcgcag gagcgcatca gtgagttttc ggctgtcttc
     5101 aattcggtgg gagagggatt gttagtcctc gactcttcgg gtcaaataac cctggctaat
     5161 gaggcaatcc gcatccttac tggtctctcg ccgacggaat tttctggtca gcggttggtt
     5221 gacttgcctg agacgatatt ggcttacctg gggtacaatt cggagcaagc agaagctctg
     5281 gtaggtgcta tggggcaagg acaggtgctg gcttctcctc gagccaccat ccgcattagt
     5341 gaactgaact cggaaagaat cctggagcgc acatcctttc cagtttggga ccgccgaggc
     5401 cgcgtgacgg gttggatgat agtgcttcgc aacatgaccg aggagattca gattactcag
     5461 gccaaggatc tgatcacgga aacgcttgta cacgatctac gctcacccgc cagcgctgtt
     5521 ctgggagctc ttgacgtact aaaggactca ctcaaccatg atcaaactga aaatgagatc
     5581 gtggttcaag cgattcaggt agctcggcgt ggggcgcaac gtatgttggg gatgactgaa
     5641 tcgttgttgg agatttctcg tctccaatct ggtaagatcg aaaccaattt gctaaacttc
     5701 tcgcttcgac ccatggctac cagcgtgcta aacgatttca agaccgacgc gctagaatac
     5761 ggcatcattt tgcacaatga catccctgac gatttgtcag ccgtgcgcgc cgatctaaat
     5821 aagaccactc gtgtgctcac caatttggtg gataacgcac ttaagttcac ccctgcgggt
     5881 gggcagatca gcttgagcgc cgcccatctc acggatggat ttatcacagt aaaggttagc
     5941 gacacgggac cgggtgtgcc cgagggctac cgggagaaga tatttgagcg cttcagccaa
     6001 gttccgggct tggtgggtag gcgacgaggt tcgggtttag ggcttacctt ctgccgcctg
     6061 gcaatcgaag cacaaggtgg caagatatgg atggagcctc gtcctggggg cggtagcatc
     6121 tttatgtata cccttccagt ctcccgtgcg tagagttaaa tttctgtaga cccccaaaca
     6181 actcatttca cttaatgaag tttcccctga ttgggggatt aaatcatttc atataaattt
     6241 ctgatgcacc ttccccttgt caacctattt ccagcgcgct ataatcaagt tttgtgatcg
     6301 gtcgcaatag ataaggtaag aattaatgcc tgtatatacc tatcggtgtg aaaattgtgg
     6361 tgtgcagttt gatcgacatc agtcatttag cgatctgccg cttacccgct gtccggagtg
     6421 tagtaaaaag acactgagaa aagtctacac gcctgtagga attgtattta aagggtctgg
     6481 cttctacgcc actgacaacc gctctccatc tgggtcgagc cgctcttcta cgggaagtga
     6541 gaaaatgagt gagggtaaga aggataagaa ggagagcgaa tcgagctcat caaccaattc
     6601 ggatggtaag gagaaatctg cataatggta gaagatagct ttaggctatc catttaacaa
     6661 tctacgcttg agacgcctgg atattgaggc gtctcatttt ataaataagg agacgctatg
     6721 aagaacgaag aaatgcttgc tcgtatccga gaggatcaag ttaaatttat atcgttgcaa
     6781 tttactgatg ttactgggac ggtcaaaagc gtggatatcc ctagtgggcg tttggaatcc
     6841 gcattagagg atggtgtatg gtttgatggc tcttcggtgg agggttttgc ccgtatccag
     6901 gagagcgaca tgcgcttggt gattgacccc agcacctatg ctgttctgcc gtggacaccc
     6961 caagaactgc ggcgggcgcg tgtattttgt gatatctact tgcccaatgg gaatccattc
     7021 ccaggcgatc cacgcggcac gcttaagcgt atactggcgg agattgagtc tcgcggctgg
     7081 ttatttaatg taggacccga acccgagttc ttcctcttcc gaaggaacgg agagaaaccc
     7141 atccaccctg tgccacatga tgtgggtggt tatttcgatt tctctgccaa cgatgatgca
     7201 gtgcgcgtac gcacagaact tatgaatgcc ttatcgggaa tgggtctcga tgtggagatg
     7261 ggacaccacg aagtggctct agggcagcat gagatcgatt ttcgctatgc agatgcatta
     7321 cgggctgcag ataatattct gaccctcaag tacactgtta aagctattgc cgctcaacat
     7381 ggattagtgg cttcattcat gcccaaaccg atatttggga taaacggatc gggtatgcac
     7441 tgccaccaat cgctttttaa taaggaaggt gagaatttgt tcttcgatgc aaacgatgaa
     7501 tataatctca caaagctagc ctatggtttc atcgctggtc aactggggca tgcacgcgcg
     7561 ttagcggcgg ttgtctctcc aacgatcaac tcttacaagc gacttgtacc gggttacgaa
     7621 gccccggtgt atgttggttg ggcgcagatc aatcgctcag cgctcatccg gatcccccgc
     7681 tatacgcctg gtagggagaa atcttcgcga gctgaactgc gctttccgga tccatctgca
     7741 aatccatatc tggctctcac cgttatgctg gcagccgcat tagatggcat cgataacaat
     7801 ttgccctgtc cgcctccgct caataatgta aatgtttatc atttgacgcc tgaggagcgc
     7861 gtggcgatga atattcccga gttgccgggc tctttggccg aagccttacg agaactcgag
     7921 aacgacaaag ttctccaggc tgcactcgga gcagaggcct atgaagcctt ttaccgtgcc
     7981 aaatgggcag aggtagaaga ataccgcatg cgcgtgacag attgggaagt cgagcgctat
     8041 ctcgagacag cataataacg aaaaaaacgg atggcgaaag gattcttgct ttcgccatcc
     8101 gtttttaatt cctgcttcac tcaattgtta ataaggttac ccagtgggat tcaccgcgga
     8161 tacctggatt accatgcggc aaaatgcgca ctctcccggg gcagttgtcg gttgaccaca
     8221 actgggacag gagtttaata tttcacctga agcgccgatt tctgccaatg tctcgggtgg
     8281 ggaggtgaac aagccgcttt ctttggcctg caagaaggac aggtaaaaac tcagcttcgc
     8341 acctggacgg tcggcttcta gcttattgag aatttctttg tagtagatgg cctttgatcc
     8401 ctcgacgtaa gggcactcat cgtagatgta gtcgataccc cgcacgaggg cataagcagc
     8461 catctcgcgc tcgtagaagc ggcaaagtgg cttagctttc cgcaccatcc cttggcgcgc
     8521 ttgcagcact gggctctgac gcatgagata gcccggagac cagttcaatg tattggcaaa
     8581 taatacggcg gcttcatcat ccaggttatg tccagtgacc agtacatcgt agccttcgtc
     8641 acgggcgatg cggttcatca catggcgttt ggtcaggccg cataccgaac aagggcgacc
     8701 cttgccgcgt gtggtgcgct cagctatctc agggatggtc tctccataag tctcatgcac
     8761 atctactacc cgaaggtgaa ggttgcgctc aatagcaaat ttgttcgcca ggcggtgcga
     8821 ctcgtcggag tagtggagtc ctccgtcaat acccaggcca atgtacaagc catctgcctt
     8881 gtaacctaat tggtgcaaga tatcccacaa ggagagagaa tccttgccac ccgaaaccgc
     8941 aactaacaag cgatcttctt gggagaacat atgatacttt tcgatgaagc gctgggtttg
     9001 ctctgcgatc cagatgggaa aatgttgctt gcataaggcc agtttatgct ggcgcatatt
     9061 gatgatggct ttggcttcgc atttacgaca tttcattttc tatcctaacc aataattttt
     9121 tcccttatcg cgatgaacaa cacctcatcc gtccacgtct tctgtagtcc gccctcctga
     9181 aattacggcg accaacttaa tgacatcatc ttcgagtagg atttcgtcat cggtgatgag
     9241 ctcacccgcc cgcgtcgcca gaaccgtttc gctgttgact ccgatttttt caatggcgtg
     9301 gcgaacggtg gtgccggact taacaatata ttccttgtca cgtaatatca atttagcagt
     9361 catggcgatt gtttggcgct cacgaccacg caaaatggcc ttcactccac tggtgagggg
     9421 agggttggtt ggttgagctc gatgaattca acttgcgaat aaattccagc atcacgcgag
     9481 taaccagctc ggggctgctg atatgaggtt ggtgtccagc ggactgtatt ggataaccaa
     9541 atgcattggg caaggcttgc accaattgcg ggaatgatgt tgggtgtagc gtctggtctc
     9601 gtttacccca gatgaccagc actggaaggc tgacctgtgc aagctggtca gacagatcgg
     9661 gcatctcgtg tgttatataa acgaaatgcg gtgacgcacg cttgtaatca gcgacgatct
     9721 gttggcgggc tttggcggag aagttggtca tggtctcggg attccagccc attaccgcac
     9781 ggatcatcca gtgcggtgtg acccgcatgg ctttctcact aagagccgga cggcgacgca
     9841 ccaaccgtaa taataaggat agttgctggg ctttatagta tggattgata agaaccaaac
     9901 cgcgcacctg ctctggatgg cgcaaggagt ggaccaggct tagatacccc cccaaagaat
     9961 gacccactag gatcggagga gaatcgatat cgtggctatt tacccattct cggaagtgat
    10021 cgtaaagcgc ttcgacctgg tatacctgcg gatcgttcgg cttggcgctg tccccatgcc
    10081 ccagtaagtc caaggcgtag gcagagtaac cgttggcagc cagagaagga gcaatttcag
    10141 tccaatcata aagcgaggcg gccatgccat gtattaagat gaccggctga cctcttccga
    10201 gtgagatatc gttggcgtga aattcgttca ttctaattgt tgaaatacct gagactaaat
    10261 gatgggctaa tcttattcgt cgattgtttg ctccccttct tccttcgtcc tctcctcaaa
    10321 gcgctttgcc agctcccgtc gtaacacttt tccgatgaaa gatcttggaa aatcgtcgac
    10381 aaatatgacc aggcgcggca ctatttctgg tggtaagcgg cgcttgcaat aggcaaccag
    10441 cgcatcagct gtggggcgtt ctgaacgggc aatgataaag gcaatcggct gcccggcaac
    10501 cgctacaacg gcagcttcct ttacctgggg tacttcaaag agcacttcct ccacatctcg
    10561 cgggaaggca ggctctccag gctttgaagg ataccacatc tccgcctttc tagcgataat
    10621 acggaaatac ccatcgctgt ccatctgagc cacatcaccc gtcagcaacc agccgtcggt
    10681 ggttagcaca cggcgcgtct cttcctcatt ctgccagtag ccaagcatta cttgtggtcc
    10741 gcgtactgcc agctcgccaa tctgaccaac acgaacctct ttcttcccat tcctcaggtc
    10801 aaccacgcgg gcttctgtgg acgggaaagg aacgccgatg ctacctacct tgcgcaagcc
    10861 ttttagcggg ttgccatgtg ttacaggcga ggcctccgtt aatccataac cttcaactag
    10921 ccggccacgc gtcagtttct cgaaagcctc ctgcacctct accggtagag gtgcagatcc
    10981 gctaatacag gccttgattg agctcacacc atagcggcgt acacctggat agtctttgat
    11041 cgccacatac atctgcggga caccagggaa tattgttggg cggtattgct tgatcgtttt
    11101 caacacatcg ttaatttcaa acctgggttt caagatcagc gtggcaccca aagcgatggg
    11161 aacattcagg gcagtggtca gcccatagct gtgcgagaag ggtagcgcgc agagaaaacg
    11221 ctcacgcccc tcgctcgcat caggaatcca atggcgggtt tgcaaggcgt ttgccactag
    11281 gttttgatgc gaaagcatca ctcccttggg cacatcggtg gtgccgccgg tgtaaataat
    11341 gacagccaga tcgttggggg ttatgggtac ttctgg
//
LOCUS       DBNGSX010000053         3120 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1526919, whole genome shotgun sequence.
ACCESSION   DBNGSX010000053 DBNGSX010000000
VERSION     DBNGSX010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1526919"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /gene="cysE"
                     /locus_tag="AC16CB_01660"
     CDS             complement(<1..536)
                     /gene="cysE"
                     /locus_tag="AC16CB_01660"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011604498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - L-serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - L-cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HIR3341201.1"
                     /translation="MWKTIRRDIAAVFERDPAARSTLEILFCYPGLHALWGHRLAHWL
                     WTRRLKLLARWVSQLARGLTGIEIHPGASIGPGFFIDHGMGVVIGETSEIGADVTMYH
                     GVTLGGTSLSKGKRHPTIGNRVVIGAGAKVLGAITVGDDSRIGANAVVVKSVPVNSVV
                     VGVPGQIVSRSRPHLATDA"
     gene            complement(540..1472)
                     /gene="cysK"
                     /locus_tag="AC16CB_01665"
     CDS             complement(540..1472)
                     /gene="cysK"
                     /locus_tag="AC16CB_01665"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019344 - L-cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HIR3341202.1"
                     /translation="MKIANDVTELIGNTPLVRIRRLSDGAKAEIVAKLEFYNPAHSVK
                     DRIGVSMIDAAERAGLIGPETIILEPTSGNTGIALAFVCAARGYKCVLTMPDTMSMER
                     RLLLRAYGAELVLTPGSEGMAGAVRKAEEMAASDSRYLIPQQFKNPANPEIHRRTTAE
                     EIWRDTDGKVDILVAGIGTGGTITGVGEVLKKRKPSFRCVAIEPAASPVLSGGQKGPH
                     MIQGIGAGFVPDVLNTQIYDEVVRVTNEDALETARRMAREEGLLVGISSGAAMWAAMQ
                     VARRPENEGKLIVVIIPSFGERYLSTALFAELAG"
     gene            complement(1494..1784)
                     /locus_tag="AC16CB_01670"
     CDS             complement(1494..1784)
                     /locus_tag="AC16CB_01670"
                     /inference="COORDINATES: protein motif:HMM:NF023174.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HIR3341203.1"
                     /translation="MSLYFVRHQHSPETCPAKDPQMGSMLLTHINPMNARKFGVNLIG
                     DAVLDGQHTFVVIAEADSPANIENFMQPFKMAGPVEISPASTCEVVVERLGC"
     gene            complement(1787..2278)
                     /locus_tag="AC16CB_01675"
     CDS             complement(1787..2278)
                     /locus_tag="AC16CB_01675"
                     /inference="COORDINATES: protein motif:HMM:NF025819.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008235 - metalloexopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HIR3341204.1"
                     /translation="MKREVIAARISLELQDLVINMLEIASEVLKKIHAHGEKAYPEEG
                     AGFLLGKVDGEQRRVTAILELVNAREDAARHNRYLLTPQDYLRGEQEADRLNLDVLGV
                     FHSHPDHPSLPSEFDRQWAMPWFSYLITSVQAGRAAGSRVWRLVEDRSQFDEEKIEIT
                     NRS"
     gene            complement(2275..>3120)
                     /locus_tag="AC16CB_01680"
     CDS             complement(2275..>3120)
                     /locus_tag="AC16CB_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIR3341205.1"
                     /translation="AEISPRVQVLAKAEWFNPGGSIKDRPALSILRFAQEEGLLRPGV
                     RLLDSTSGNMGIAYATFGATLGVPVTLVLPANASPERQTILRALGAELILTDPLEGSD
                     GAIRFARQLAIENPETYFYANQYDNPANWKAHYHATGPEIFQQTGGRITHFIAGLGTS
                     GTLTGVGRYLRQIGTQVQLIAVQPDAAFHGLEGLKHLPTAIQPGIYDSSMPDRILEIS
                     TEAAYAMVRRLAREEGLFVGISSGAAAVAALQVAAELDEGVVVTVFPDAGYKYLSDDA
                     LWDKA"
BASE COUNT          683 a          951 c          856 g          630 t
ORIGIN      
        1 gcatccgtag ccaggtgcgg tcggctgcgc gatacgatct gtccgggcac gcccaccacc
       61 accgagttga ccggcaccga cttgaccacc acggcgttgg ccccaatgcg gctgtcgtcc
      121 ccaacggtga tggcgcccaa caccttggct cccgcgccga tcaccacccg attgccgatc
      181 gtgggatggc gtttgccctt ggagaggctg gtacctccca aggtgacccc gtgatacatc
      241 gtcacatcgg cgccgatctc agaagtctcg ccgatgacca cacccatgcc atgatcgatg
      301 aaaaagcccg ggccgatgga tgcgccaggg tgtatctcga tgcctgtcag gccgcgcgcc
      361 agctgcgaaa cccagcgcgc cagcagcttc aggcggcgcg tccacaacca atgcgccagg
      421 cgatgccccc ataaagcatg cagcccagga tagcaaaaca agatttccag ggtactacgc
      481 gccgctggat cgcgctcaaa tacggcagcg atatctcgcc ggattgtctt ccacatggtt
      541 caccccgcca gttcagcaaa tagagcggtg ctcagatagc gctcgccgaa ggaagggatg
      601 atcacaacga tcaatttacc ctcgttctcc gggcggcgcg ccacttgcat ggccgcccac
      661 atggccgccc ccgaggagat gcccaccagc aggccctctt cgcgagccat acgccgggcg
      721 gtctccaggg cgtcttcgtt tgttacgcgc acgacctcgt catagatctg cgtgttgagc
      781 acatcgggca caaagccagc gccgatgccc tggatcatgt gtggaccttt ctgcccgcca
      841 gagagcaccg gcgaggcagc cggttctatt gccacacatc ggaaggaagg cttgcgcttc
      901 ttgagcacct caccgactcc ggtaatagtt ccacctgtgc caatgccggc aaccagaata
      961 tcaaccttgc catctgtatc tcgccaaatc tcttcggcgg tcgtccggcg gtggatttca
     1021 gggttggccg gattcttgaa ttgctgaggg atgagatagc gcgaatctga ggcggccatc
     1081 tcttccgctt tacgaaccgc gccagccatc ccctcgctac ctggcgtgag caccagttca
     1141 gcgccgtagg ctcgcaggag aaggcggcgt tccatgctca tcgtatcggg catggtaagc
     1201 acgcacttat agccccgcgc tgcgcagacg aaagccagag caatacctgt gttaccgctg
     1261 gtaggctcca gaatgatcgt ctcgggaccg atcagccctg cccgctctgc ggcatcaatc
     1321 attgacactc caatgcgatc cttaacgctg tgcgccgggt tgtaaaactc taacttggcg
     1381 acaatctccg ctttggcgcc gtcgctcaag cggcgaatgc gcaccagggg agtattaccg
     1441 atcaattcag tgacatcatt agcaattttc atctctgtct cccccgctca agactagcaa
     1501 cccaggcgct caaccaccac ttcacaagtg gaagcagggc tgatttcaac aggaccagcc
     1561 atcttgaacg gctgcatgaa attctcaata ttagccgggc tgtcagcctc agcgatgacc
     1621 acgaaagtgt gctgaccgtc caacacggca tcgccgatca agttgacgcc gaacttgcgg
     1681 gcattcatcg gattgatatg tgtaagcaac atcgagccca tctgcggatc tttagccggg
     1741 caagtttcag ggctatgctg gtgacgtaca aagtaaaggg acatggttaa ctcctatttg
     1801 taatctctat tttctcttca tcgaactgcg accgatcttc taccaagcgc cagacgcggc
     1861 tcccggccgc gcgaccggcc tgcacgctgg taatcaggta ggaaaaccag ggcatagccc
     1921 attggcggtc aaactcggaa ggcaggctgg gatggtcggg atgggaatgg aacacgccca
     1981 gcacatccag attcaatcga tcagcttcct gttcgccacg caggtaatcc tgaggggtaa
     2041 gcaggtagcg gttgtgccgg gcagcgtcct cgcgggcatt gaccaactcc aggatggcag
     2101 tcacccgacg ctgctcacca tccactttgc ccaacaagaa gcccgctcct tcttcgggat
     2161 aggctttctc accgtgagcg tgaatcttct ttaaaacttc agatgcaatt tccaacatgt
     2221 ttattaccaa atcttgtagc tcaagcgaaa tgcgagcagc gattacctct cttttcaagc
     2281 tttatcccac aaggcgtcat cgctcagata tttatagccg gcatcaggaa aaacggtaac
     2341 caccacgccc tcgtccaatt ccgcagcgac ctgcagggct gctaccgccg ctgcgccgga
     2401 ggagataccc acaaaaagac cctcctcacg agcgagccgc cgcaccatcg cataagctgc
     2461 ctcggtagat atctccagga tacgatcagg catggaggag tcatagatcc ccggttggat
     2521 ggcagtaggc aggtgcttaa ggccctccag cccatggaaa gcggcgtcgg gttggacagc
     2581 gatcagctgg acttgtgtgc cgatttggcg cagatagcgc ccaacgccgg tgagcgtgcc
     2641 agacgtgccc agcccggcga taaagtgggt gatccgcccg cctgtctgct ggaaaatttc
     2701 tggcccggta gcgtggtaat gagccttcca gttggcgggg ttgtcatatt ggttggcata
     2761 aaaataagtc tcaggatttt cgatggcaag ctggcgagca aagcggatgg cgccatcaga
     2821 gccttccaag gggtcagtca ggatcagttc cgctcccagg gcacgcaaga tagtctgccg
     2881 ttctgggctg gcattggcag gcagcaccag ggtgaccggt acacccagtg tggctccaaa
     2941 agtggcgtaa gcgatgccca tatttccaga ggtggaatcg agtaagcgca cacccggtct
     3001 taacaagcct tcttcttgag caaagcgcag gatactgagc gccgggcggt ctttaatcga
     3061 gccacccggg ttgaaccatt cggcttttgc gagcacctga acgcgcggtg atatctccgc
//
LOCUS       DBNGSX010000054        16901 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1526935, whole genome shotgun sequence.
ACCESSION   DBNGSX010000054 DBNGSX010000000
VERSION     DBNGSX010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 16901)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16901)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16901
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1526935"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            288..1220
                     /locus_tag="AC16CB_01685"
     CDS             288..1220
                     /locus_tag="AC16CB_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR3341206.1"
                     /translation="MYSEIEKAIKILQSVANTEVESKLVCEVIQAYIEAKASVLSSHT
                     MRDYGLTFRRFCDFLPDPRPIHEISAKEVRSFLNTIPGGRKNRKNAHIALGALWSFAV
                     GEDFTHEHLIRKVEVAKPEVRAIIPFSKEEIEKLLRVARTSGREKLRNVAVIKTLIDT
                     GVRASELCGLKLEDLEGDYLRVMGKGSKERRVPVSSLAMASVVEYLGARPPGKRNTPV
                     FLSEKGGALTRDSLRLLINRLAQRAGVNNAHPHKFRHTFALNYILNGGDPYSLQMILG
                     QSTMDMVKRYLYLSNRDISAVHNRVSPLKNWGLD"
     gene            1531..2109
                     /locus_tag="AC16CB_01690"
     CDS             1531..2109
                     /locus_tag="AC16CB_01690"
                     /inference="COORDINATES: protein motif:HMM:NF019844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR3341207.1"
                     /translation="MESGFFFPLIGVLLSLLWVYFSFRYYLYYRGLIYNRESNQEKSG
                     RLMGRAQLSLLVSGLVFIAMLVTLFSLSGTAISPPVAATPAPGEGADQLLPTPSPVME
                     LVLTPSPVSSPTPEVASSSGFARIGNTNTFGVYVRSEPGLKYEIVTQLSDGNRVELMG
                     EAQLADGFNWQQVRLEDGRVGWVADNFLIPEP"
     gene            2254..2706
                     /locus_tag="AC16CB_01695"
     CDS             2254..2706
                     /locus_tag="AC16CB_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341208.1"
                     /translation="MSPERLESEAEWEVWYQDTFDQESARQVEVVGQGLVEGLIELWA
                     RHLFETVQASGQQGFSRFNLWLKQERRSIEVVGEWDGQVRLRGWVFAGERQANTGYAE
                     KGDKSLLNLIAASHKDLILRGETSEAILATAKASGGREDFVERLLSMK"
     gene            2857..3540
                     /locus_tag="AC16CB_01700"
     CDS             2857..3540
                     /locus_tag="AC16CB_01700"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016107.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR3341209.1"
                     /translation="MDKKLIARFAIRETMGVVIMGVALFWSAGRIDWWPAWALLAVML
                     AWIIAMGFAILRFNPDLLAERLGPRKGGKHWDLIIMGIVGLAELARLIIAGLDQRYGW
                     TGGFPIAAQIAALAVCALGYALGVWATASNAFFSQIVRIQTERGHAVATGGPYQYVRH
                     PAYVGTILYQLAMPVLLASWWALIPGGVNAILLVIRTALEDRTLQAELSGYADYARLV
                     RHRLLPGIW"
     gene            3566..3943
                     /locus_tag="AC16CB_01705"
     CDS             3566..3943
                     /locus_tag="AC16CB_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341210.1"
                     /translation="MKDYAIELEIFEGNGGQPRTDGTHPDFAREGICAWMYGGLGETS
                     YQVGQKFRYPKDLGELCPWLVDSLTGIIRALEHGGTLSWKYQGTPYEKVIDPDGVTTE
                     YVRCLDPTASGIVIKVTRTALPD"
     gene            4091..4294
                     /locus_tag="AC16CB_01710"
     CDS             4091..4294
                     /locus_tag="AC16CB_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341211.1"
                     /translation="MITEYPLTKPNRVKLARAFKHVPRVDLSIQCAIEGLMGGDRRRL
                     RLVYRKKRAWRSRSILLEEAAYL"
     gene            4307..4579
                     /locus_tag="AC16CB_01715"
     CDS             4307..4579
                     /locus_tag="AC16CB_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341212.1"
                     /translation="MGEGGDSGGGVWHDGQLAGNLWATIGHKKDPIAAMLTGTAVQSA
                     LKATNKSYAAILPVLTQFTQPAVGSASEVGGVISIGSPYKNIIGAE"
     gene            4771..6843
                     /locus_tag="AC16CB_01720"
     CDS             4771..6843
                     /locus_tag="AC16CB_01720"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR3341213.1"
                     /translation="MADRPTWVGKTLGGRYRIDDILGQGGMSAVYKAYDPNLKRVVAV
                     KIIHGHLADDQRFMARFEEEATAVAQLRHPNIVQVFDFDHDGDLYYMVQEFIPGETLQ
                     QLLRRLERSGRRMPLGDVIRYTVHIANASGYAHQRGMIHRDIKPANIMIDVHGQATLM
                     DFGIVKITGGERHTATGAVVGTALYLSPELIRGEVPDPRSDVYALGVTLFEMVSGRPP
                     YEADSAMTLMMMHLNDPLPDLRQLRHGVPDALVAVIEKAMQKNRPDRYASMDEMAADL
                     QAALQTLPASPDVTQVEAAPVKSEPTSPTAALRDTGTQVEVTPEPEPVAETPPPSPAA
                     PPAQMPVSQPPTLDSPSIVTPPEQVASAELGTQAMPAVEPSVTPSPGADETLPPVERG
                     AGGPPAPPPTATSAPSAGKKPVPMAVWVGAGLVVVILVIFGIIQASRGGSQPSQALEP
                     AASATPTTAVVVAAAPTDTLAPTETAAPSATATLAPTDTPSPTPTPTLGLPPTPTFPA
                     GVPFAFIKSITLNEGQNYIVDYDTLEFQEQLPGVHVHFFFNTVLPEQAGNPGSGPWIL
                     WGGPRPFDKYRQRDHNEQATQMCVLVANADHSVQPESGNCAILPDVNAVTILTETACL
                     AGTDVSSQVVTSLPLGQVARVLGISTDGAWWNIANPEDMAANCWVPGDLAFFHGDQAS
                     VPVVEPVD"
     gene            6888..8576
                     /locus_tag="AC16CB_01725"
     CDS             6888..8576
                     /locus_tag="AC16CB_01725"
                     /inference="COORDINATES: protein motif:HMM:NF014503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR3341214.1"
                     /translation="MDTTYGKLILLNPNGPEQEFELAKASVSLGRAITNDIILSDVRI
                     SRNHARLEGGSQGCTLVDLGSSNGTRVNGIQVERATLKPGDTISLGSQQLRYEIADIS
                     EDLGLTQIDTQAQLEQTMDREFLPQAINETGNPSLVIFTGDRTWRVDLEDQATIGRDE
                     SCTVFIDAANVSRRHAEIVYTGGIYVLKDLGSTNGTWVRDQQVDQLILQDGDVMRIGP
                     AQIIFKGGFQEQALTMADEQLAKPTGRRTVVFVPGSMGSELWLGNERVWPNVKTIFTN
                     PEIFIYPSRVPLEPRGIVDEVVIVPNLIKQDQYNRLGDYLVEELNFRRGVDFFEFAYD
                     WRQDVRISARQLGELIQSLPDSQPLVLIAHSLGTMVSRYYIERLGGHKRIERAILMGG
                     PHQGSTKILTSMLIAPEVLPFGIMGERLRQIVLTFPSTYQIIPTYEVGTDQDGKKINF
                     LDDESWLAPEFVPLLRQGREFRSELGSMLSFPYVSVFGYGLKTISDVTIARDAAGKVQ
                     KVDYISGNAGDNSVLEQSAFLQGSEIHPVRQHHGSLFVDNDVKMRLKLELTRPY"
     gene            complement(8573..9814)
                     /locus_tag="AC16CB_01730"
     CDS             complement(8573..9814)
                     /locus_tag="AC16CB_01730"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3341215.1"
                     /translation="MMVNSAKSGRRQIYILAFVLLVVMLGFGVVIPIIPFFIEDMGAG
                     GIELGLLVASYAIMRLIFGPIWGSLSDRVGRKPILMVGIFGYGITMILFGLATKLWML
                     FVFRSLSGILSSATSPTTMAYISDSTSEKDRGGGMGTLGAAIGIGTILGPGFGGLLGG
                     ESLSIPFFIAGGMSFLALLLIWLFLPESLSVEARRQASHKKRLTASDLWQAMVGPIGI
                     LLGMAFLVSYAMTTFYSIFGLYALQKFSYGTQEVGAIMMVVGMVSALAQGVFTGPLTR
                     RWGEAAVIKTTLLATALGFIGISLGNTFVTFLIAIGFFTLATALLTPAITALTSKRTT
                     LEQGISMGLSNSFMSLGRIAGPMVAGITFDINIEYPNLSGALVLLVGFLASLVWVKKA
                     APTPKTADKALESPQPAISRH"
     gene            complement(9848..12475)
                     /locus_tag="AC16CB_01735"
     CDS             complement(9848..12475)
                     /locus_tag="AC16CB_01735"
                     /inference="COORDINATES: protein motif:HMM:NF013491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR3341216.1"
                     /translation="MTPYVLSFTDAEASLETVGGKGASLARLAGAGFPVPGGFHVTTQ
                     AYRQFVSANDLQTRILAALEEVDISIPNTLEKASHTIREAFLQGQIPPEIANDIVQSY
                     AELPGTNPAVAVRSSATAEDLPDASFAGQQETYLNVSGAGAVLEATKKCWASLWTARA
                     IGYRARQGIHAEGVALAVVVQLLVPAEAAGILFTANPMNGRRDQVLISASWGLGEAVV
                     GGLVTPDSLIMDKASGNVLERQTADKQVMTVRVDGKTEEQPIPENLRRIPVLDDEIAT
                     ELTRLAVQIEDLYGMPMDIEWTWTDGEFSIVQARPITALPTSETLASVEWTLPDPKGQ
                     YMRTSIIDFLPDPLTPLFATLGVSELNVGIKRLIAYMVGKETQDANWPDDSILTINDY
                     AYFAAKYTPRQMLWIIFVLVPKFPSLLRSGIPRWRDEVRPYYLKTVERWEDKPLVSLT
                     PSEIFSGVHEVMEAAIDHLGSLMVGTMGVAAGTEMLFTRVYEKMIKKDGDLSAPAFLM
                     GYDSIPIRSEKTLYDLAKWSRNIPDLAVYLSETPTLQIRAALDSNIAPLSVNVDTWHE
                     WQERFQSYLDAFGHLIYDLDFAKPLPKDDPTSMLETIKMYLRGEGTNPHERQQVAAER
                     RLQASENTLRRLKGLRRWAFRTTLKLAQRQAEVREDGIADIGLGYPLLREMLFELGSR
                     LVEAGAIQQADDIFWLEQAEIETLIAAMEQGSAINSLADSMQERKALWKSKKLLVPPP
                     TLPPTKKYMGFDIEAFVGAGEIGQVGDTLKGVATSAGRVTGTARVLHGPQDFDQMQAG
                     DILVAKITTPAWTPLFAMASAVVTDIGGPLSHGSIVAREYGIPAVLGTGVATHRISSG
                     QKIAVDGDAGTVTLLNGHE"
     gene            complement(12530..13123)
                     /locus_tag="AC16CB_01740"
     CDS             complement(12530..13123)
                     /locus_tag="AC16CB_01740"
                     /inference="COORDINATES: protein motif:HMM:NF036525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3341217.1"
                     /translation="MPKGIPLTEEEHARRRREIFDATIHLILEKGFTETSMREIAIEA
                     GVGKSTLYDYFHTKEDLLVFVVEEQLNHINHIALDIASQDGSAVERLRQLMQIHLDYL
                     VKNKNFLLQLMLEIQRLSPASQKKIQLKRYAYQDLLRSIIEQGVAQGSIRQVDPAMVA
                     KILIALMTPVVFTSRPVGSPEQMLSHALDIVMNGIQA"
     gene            complement(13244..15238)
                     /locus_tag="AC16CB_01745"
     CDS             complement(13244..15238)
                     /locus_tag="AC16CB_01745"
                     /inference="COORDINATES: protein motif:HMM:NF023128.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3341218.1"
                     /translation="MAASTSALIQIDEKEYSRRIWAWTMYDWANSAFATTILAAVLPI
                     YFSQVAGANLPSAATATAYWSTGLSVSLLIIAVLSPILGTISDVMRGKKPFLAVFAGI
                     GIVSTALLILVGTGDWVLASVFGVLGRIGFSGANTFYDALLPHVAREADRDKVSARGY
                     AMGYLGGGLLLAINIVMIQMLPGTWGARLSFFSVAIWWAVFSIPLFLRVPEPPAATAK
                     LDSGENVISVSFKRLAETLRDIRQYRELFKFLLGFLIYNDGIGTIIGVAAIYGAELGF
                     GSLELILALLLVQFVGIPYSLIFGRLPSQNEKRRPFFLAYVLFNLVALPLVGIAGTRL
                     LPSQITGTPPARFASSPTAAGQGIYLATDNRLKTTGTWSDLEVSAAELGGETSATYRA
                     GSDAEARASFTFNGEKVELTYSSGPQGGIWAVLMDGQPLIDPDSGEPLTIDAYHPTVR
                     YGEEVVLAAAAPGEHTLVLANTGRKAPESQGTQISLAQIEVLPPSRQSNLLVVIGLIA
                     AVEIIGLLISWVLGPPLFSRLAATIDTRRAILLALSVYAVISVWGFFLDSVIEFWFLA
                     WMVSVVQGGSQALSRSLFSTMSPASKSGEFFGLFGVMEKFSAIIGPLVFAFAVANFGS
                     SRPAVLSIVVFFILGGYLLTRVNVEEGQRVARAEDAALLG"
     gene            15647..16717
                     /locus_tag="AC16CB_01750"
     CDS             15647..16717
                     /locus_tag="AC16CB_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HIR3341219.1"
                     /translation="MSVEFIFRLVGMVVFAIMGAYFGVYLAAISNGPLDMYGVVVALT
                     GALAGLILTPYLTTRPARYMRAVFTRISAQTLAASLTGLIAGLIIAALLAFPFSMLPD
                     PFRAILPFIGVLVFGYLGVAVFVMRQNDIFSILRINLPGRSAPGNDSGANRDASRTIL
                     LDTSVIIDGRIADITRTGFLVGTLMIPRFVLNELQYIADSADGLRRQRGRRGIEVLSQ
                     LQKDTTIPVRISDIDIEGTREVDDKLVILARQLRCPILTNDYNLNRVAELQGVPVLNV
                     NELANAVKSVLLPGEALAVNIIQEGKESGQGVGYLDDGTMVVVEDGRGQVNSKATVVV
                     TKVLQTAAGRMIFARLDQRGEL"
BASE COUNT         3795 a         4556 c         4764 g         3786 t
ORIGIN      
        1 tagcgccccc tgctaggccc aggaaaacag ccaaaacacc caccattaat agtgttccaa
       61 gtgaatgcat agtccctcac aaatgattga ttcgttcaat caccagggtt acggctatga
      121 tcgtcaaagt agttatgacg aacaccagcc caaagaggat tagtttgatt tttccgttca
      181 tttcgtactc tttcaattag ctgcaaatta gctgtgctcg ataaatgtct atcatcggag
      241 caaaccccgc gaaccttgcg aaaaaaactg acagaatggg aaacctgatg tatagtgaaa
      301 tagaaaaggc catcaaaata ctgcaaagcg tagctaacac agaagtggaa tcgaaactag
      361 tttgtgaagt aatacaggca tatatcgaag ccaaagcctc agtgctcagt tctcacacaa
      421 tgcgggatta tgggcttact ttccggcggt tttgtgattt tctgcctgat ccgcgcccaa
      481 tccacgagat ttcagcgaag gaggttcggt ctttcctcaa caccattccc ggtggtagga
      541 agaaccgaaa aaatgcccac atagctttag gcgcattatg gtcttttgcg gttggggagg
      601 atttcaccca cgagcacctg atcaggaagg ttgaggttgc caagcctgaa gttcgggcga
      661 ttatcccctt cagcaaggag gagatagaga agctgcttcg cgttgctcgg accagtggga
      721 gagaaaagct gagaaacgtg gcggtaatca aaacgctgat tgacaccggc gtccgagcgt
      781 ctgagctttg cgggctgaag cttgaagatc tcgaagggga ttacttgcgg gttatgggaa
      841 aaggatcaaa agaacggcgt gttccggtat catccctggc gatggcatca gtggtagagt
      901 acctgggagc ccgaccgccg ggtaaacgaa acaccccggt atttctatct gaaaaaggcg
      961 gtgcgctcac ccgtgacagc ctgcgcctgc tcatcaatcg tctggctcag cgggcaggtg
     1021 tcaataacgc ccacccccat aaattccgac acacttttgc gctcaattac atcttgaatg
     1081 ggggcgaccc atacagcctg cagatgatct taggccagtc cacgatggat atggtcaaaa
     1141 gataccttta cctctcaaat cgggatattt ctgcggttca taaccgcgtt tctccgctga
     1201 aaaattgggg attagattga accgtaaggt catgcgcctt tattcgcgct catggttcac
     1261 atccgtgagg tcgggggttc gagcccctcc gcgcccacca cgccgatgaa ggctcccccg
     1321 tatcaaacac ttccctgggg gagttttcat ttaacgggcc attgcggctg ttgccgtaat
     1381 ggcctgcgct cgcactgcct cgcttactcc tacccacaag actcaaaatc gtcgccctcc
     1441 ggggcaggtt ggacgacgat tcttttttcc ccaaatatat cgattaaggt tttttgcgca
     1501 gggtaaaaaa tagcctaaaa tactatacta atggagtcgg gcttcttttt tcctctgatc
     1561 ggtgtgctgc tcagcctgct gtgggtctac ttcagcttcc gttactatct atattaccgc
     1621 ggcctgatct ataacaggga aagcaaccag gaaaaatctg ggcgtctgat gggcagggca
     1681 cagctatcct tgctggtctc gggcctggtt tttatcgcta tgttggtcac cctgttcagc
     1741 ttatcgggga ctgccatatc gccccctgtc gccgccacac cagcgccggg tgaaggcgcg
     1801 gaccagttat tgcctactcc cagccccgtc atggagcttg tgctcacccc ttcgccggta
     1861 agctcgccga cgcctgaagt cgcttctagc tcgggcttcg cccgcatcgg gaacaccaac
     1921 acttttggcg tgtacgtgcg ctccgagcct ggactgaaat atgagatcgt tacccaacta
     1981 tccgatggca accgggttga gctgatgggc gaggcgcagc ttgccgatgg gtttaactgg
     2041 cagcaagtgc gcctggagga cggtcgcgtg ggctgggtcg cagataattt cttaattccc
     2101 gagccttgaa ccgagcttcc gcgcagttta aagccaccgc acaatgttga tcttgaaccg
     2161 ctggtgctga agccatgcaa attgatatat actaagttaa gatgtgagag gagcgctatt
     2221 cacctgtttg cgcagaaggc acaaggaatg taaatgagtc ctgagagatt agagtctgag
     2281 gcggagtggg aggtctggta tcaagatacc tttgatcagg agagtgcccg ccaggtggag
     2341 gttgttgggc aagggttggt ggaagggtta atcgagcttt gggctcgtca cttgtttgag
     2401 actgtccagg caagcgggca gcaagggttc agccggttca atttgtggtt gaaacaggag
     2461 aggcgaagca tagaagttgt gggcgagtgg gatgggcagg tgcgattgcg gggctgggta
     2521 tttgcaggcg agagacaggc aaacacagga tacgccgaga aaggcgataa atcgttattg
     2581 aacctgattg cagcatcgca caaggatctt atcttgagag gcgagacgag cgaggcgatt
     2641 ctggcaacgg caaaagcttc cgggggccgt gaggattttg tggagcggtt gttgagtatg
     2701 aaatagtctg ctttgaatat gagcctgacc tgcccgcacc ctgctctacg aacagctgcc
     2761 ccctttgatt gttttagcca ggagcttcat gatgctgttg gcttgtagac acaccgtcta
     2821 ttctctataa gtaacccgat taggaagtga aaagacatgg acaaaaaact tatcgcacgt
     2881 tttgccatcc gtgagaccat gggggttgtc atcatgggtg tggctctgtt ctggtcggct
     2941 ggccggattg actggtggcc tgcctgggca ttgctggctg tgatgctggc ttggatcata
     3001 gccatgggat tcgccatctt acgcttcaat ccagacctgc tggctgagcg cctgggacct
     3061 cgcaaggggg gcaaacactg ggacttgatt atcatgggca ttgtgggtct cgcggaactg
     3121 gcgcggttga tcatcgctgg acttgatcag cgttatggct ggacgggcgg tttcccaata
     3181 gctgctcaaa tagctgcgct ggctgtttgc gccctgggat atgccctggg cgtttgggct
     3241 accgcctcca acgccttctt ctcgcagatt gtccgcatac agaccgagcg tggtcacgct
     3301 gttgccacag gcggccccta ccaatatgtg cgccatccag cttacgtcgg cacaattctg
     3361 tatcaactgg ccatgccagt gctgctggct tcttggtggg cattgatccc aggcggagtg
     3421 aacgcaatcc tccttgtgat ccgcacagcc cttgaagacc gcaccttgca ggccgagcta
     3481 agtggttatg ccgactacgc tcgcctggtg cggcaccgcc tgttaccagg catctggtag
     3541 tctcacccaa cgacaaggag aatatatgaa agattatgct atcgaattag aaattttcga
     3601 gggcaatggc gggcagccac gcactgatgg gacgcatcca gacttcgcga gggaaggcat
     3661 ttgtgcctgg atgtatggag gacttggcga gacaagttat caggtgggac agaaatttcg
     3721 ttaccctaaa gatcttgggg aattgtgtcc ttggctggtg gatagcttga caggaataat
     3781 ccgcgcgctc gaacatggtg ggacgctgtc ctggaagtat cagggcactc catatgaaaa
     3841 agtcatcgat cctgacgggg tgacaaccga gtatgtgcgc tgtcttgacc ccacagcatc
     3901 tggtatcgtc atcaaggtga ccagaaccgc tttgccagat tgatgatcaa gagaattacg
     3961 ttgccaccat ccaggcaccc tagaacttcc tcaaaaaggc acagcgtttt ggatgccgat
     4021 catgcctaac tccatgtgca ggcgaccctc ccatgccaaa cggcaagcgg cgcgctagag
     4081 agggctttga atgattactg aatatcctct cacgaaaccc aatcgcgtca aacttgccag
     4141 ggcatttaag cacgtccccc gtgtggactt gtctatccag tgcgctattg aagggttgat
     4201 ggggggagat cgaaggaggc tgaggctggt ttaccgcaaa aagcgggcgt ggcggtcaag
     4261 gtcgattctg ctggaggagg cggcgtatct gtaggcgagg ggcgcggtgg gtgagggtgg
     4321 cgactccggt ggtggcgtgt ggcatgatgg gcaactggca ggtaacttgt gggctactat
     4381 agggcacaag aaggatccca tcgccgccat gctgaccgga accgcagtac agtccgctct
     4441 caaggcgacc aataagagct atgcagccat ccttcctgtg ctcacacaat ttacccagcc
     4501 agctgttggg tcagccagcg aagtgggcgg tgttatttca ataggctctc cttacaaaaa
     4561 tattatcggg gcagaataaa tgcaaatctc ctgtttaagg aacatattct ctctcccaag
     4621 tacgatctaa actcgctcaa ggctcattgt cttgctgatt tccgcctcaa attatccaat
     4681 tatcctgcaa gtgcatgtat aatataagcc gtcgcgccga gtggactctc ggggtttgat
     4741 gactttattt tgttgttagg gagttaggga atggcagatc gtccaacctg ggtcggtaaa
     4801 acgttaggcg ggcgctaccg cattgatgat atcctggggc aagggggcat gtcggcggta
     4861 tacaaggcct acgatcccaa cctcaagcga gtggtagcgg tgaaaatcat ccacggtcac
     4921 ctggcggatg accagcgctt catggcgcgt ttcgaagaag aggccacggc ggttgcccag
     4981 cttcgccatc ccaatatcgt ccaggtgttt gattttgacc acgacggcga cctgtactac
     5041 atggtgcagg agtttatccc aggcgagaca ctgcagcaac tcctgcgccg cctggaaagg
     5101 tctggacggc gcatgccctt aggggatgtg atccgctaca ccgtgcacat cgctaacgcc
     5161 agtggctatg cccaccagcg cggcatgatc caccgcgata tcaaaccagc caatatcatg
     5221 atagatgtgc atgggcaagc caccctgatg gatttcggca ttgtcaagat cacggggggc
     5281 gaacgccaca ctgcaaccgg ggcggtggta ggtaccgcct tgtacctctc gcctgagctg
     5341 atccgtggtg aagtccccga cccacgctcc gacgtctacg ctctgggcgt gacgctgttc
     5401 gagatggtca gcggacggcc gccgtatgaa gccgattcag ccatgacttt gatgatgatg
     5461 cacttgaacg acccgctgcc tgacttgcgc cagttgcgcc acggggtgcc cgacgcattg
     5521 gtggccgtga tcgagaaggc catgcagaaa aaccgccctg accgctatgc atccatggat
     5581 gagatggcgg ctgacctgca ggcagcgctc cagaccttgc cggcttctcc agatgtcacc
     5641 caggttgaag ccgcccctgt gaaaagcgag ccaacctccc caaccgccgc gctgcgggat
     5701 acgggcaccc aagttgaggt cacccccgaa ccggagccgg ttgcagagac accccctccg
     5761 tcacctgctg ctccccccgc ccagatgccc gtctcccagc cgcccaccct ggacagccca
     5821 tccattgtaa ccccgccaga gcaggtggca agcgcagagc ttggcacgca ggcaatgccg
     5881 gccgttgagc cgtcagtgac gccctccccc ggtgcagatg agaccctccc gcctgttgag
     5941 cgtggtgccg gcggaccgcc tgcccctccg ccgactgcga caagtgctcc gagcgcgggc
     6001 aagaagccgg tgccgatggc ggtatgggtt ggcgcaggcc tggtagtcgt gatactggtc
     6061 atctttggga taatccaagc cagccgtggg ggatcgcagc ccagtcaagc gcttgaacca
     6121 gcagccagcg ccacgccaac tacggcggtt gtggtggccg cggctcccac cgacaccctc
     6181 gccccaaccg agacagccgc tcccagcgcc accgctacct tggctcccac cgatacaccc
     6241 tcgcctaccc ccacgcccac gctagggttg ccccccaccc ccacctttcc cgctggcgtt
     6301 ccctttgctt tcatcaagag catcacactg aatgaagggc aaaactatat tgtcgattat
     6361 gacacgctgg agttccagga gcagctgcct ggggtgcacg tgcatttctt ctttaacacc
     6421 gtcctgccgg agcaggctgg aaatccagga tcaggaccct ggatcctgtg gggcggaccg
     6481 cggccctttg ataagtacag gcagagggac cacaatgagc aagccaccca gatgtgcgta
     6541 ctggtcgcca atgccgatca cagtgtccag ccggaaagcg gaaactgcgc catcctgccg
     6601 gatgtcaacg cggtcacgat tctgaccgag accgcttgcc tggctggaac agatgtgagc
     6661 tcccaggtgg tcaccagcct gccgctcggg caggtcgcgc gtgtcttggg aatttcgacc
     6721 gacggagcct ggtggaatat cgccaatcct gaggatatgg cggcaaattg ctgggtgccg
     6781 ggcgacctgg ccttttttca cggtgaccag gcgtcggtgc ctgtcgttga accagttgat
     6841 taattcgtta attgcgtaac ttctgaagat tctgctcagg agggaagatg gacaccacgt
     6901 atgggaaatt aatcctgctg aaccccaacg gtccagagca ggaatttgaa ctggcaaaag
     6961 ccagcgtctc gttggggcgg gcgatcacta acgatattat cctcagtgat gtgcgtattt
     7021 cgcgcaatca cgctcgcctg gaaggtggct cgcaaggttg taccctggtt gatctaggct
     7081 cttccaacgg cacgcgggtc aacggtatcc aggttgagcg cgccacgctc aaaccaggcg
     7141 acacgattag cctgggtagc cagcagcttc gctatgagat cgctgacatc agtgaagacc
     7201 tgggcctgac ccagatcgac acacaggctc agcttgaaca aacgatggat cgggaatttc
     7261 ttccccaggc gatcaacgag accggtaacc ccagcctggt gatattcacc ggcgatagga
     7321 cctggagagt cgacctggag gaccaggcta cgatcgggcg cgacgagagc tgcacggtct
     7381 tcattgacgc ggccaatgtc tcccggcggc atgctgagat cgtctatacg gggggcatct
     7441 atgtgctcaa ggacctgggg agcacgaacg gcacatgggt gcgagaccag caggttgacc
     7501 agcttatcct gcaggatggt gatgtaatgc ggattggccc agcccagatc attttcaagg
     7561 gcggatttca agagcaggct ttgaccatgg ctgatgagca gctcgccaag cccaccggcc
     7621 ggcggacagt ggtgtttgtc ccaggttcga tgggctcaga gctgtggttg ggaaatgagc
     7681 gcgtctggcc gaatgtcaag acgatcttca cgaacccgga gattttcatc tacccatcca
     7741 gggtcccgtt ggagccgcgc ggtatcgtgg atgaagttgt aattgttcca aacttgatca
     7801 agcaagatca gtacaatcgc ctgggcgact acctggtgga ggaattgaat tttcggcgcg
     7861 gggtggattt cttcgagttc gcttacgact ggcgccagga tgtgcgcatc tcggcccgcc
     7921 agcttggcga gttgattcag agcctgcccg attcgcaacc cctggtcctc atcgctcaca
     7981 gtctgggcac catggtcagt cgctattaca tcgaacgcct gggcgggcac aagcgtatcg
     8041 agcgcgccat cctgatgggg ggaccgcatc aggggtctac caaaattctc accagcatgc
     8101 tgatcgcgcc cgaagtactg ccgtttggca tcatgggaga gcgattgcgc cagattgtgc
     8161 tgaccttccc cagcacgtac cagatcatac ccacctatga ggtgggcacc gatcaggacg
     8221 gtaagaagat caacttcttg gacgatgaga gctggctggc gccagagttt gttccgttgt
     8281 tgcgacaggg cagagaattc cgcagcgagc tgggcagcat gctgagcttc ccatatgtct
     8341 cggtcttcgg ctatggcttg aagaccattt ctgatgtcac aatcgccaga gacgctgccg
     8401 gtaaagtgca aaaggtggat tacatcagcg gcaatgcggg tgataactct gtcctcgagc
     8461 agagcgcctt cctgcaaggc agcgaaatcc accccgtgcg ccagcatcac ggctccttat
     8521 tcgtcgataa cgatgtcaag atgcggctca agctggagct gacgcgcccc tattaatgcc
     8581 tggatattgc tggctggggt gattccagtg ctttatcagc cgtttttggg gtgggcgcag
     8641 ccttcttcac ccaaaccagg ctggcaagga aaccgaccag caaaaccaac gctccgctca
     8701 ggttggggta ttcgatatta atatcaaagg taatccctgc aaccatcggt ccagcgatac
     8761 gccccaggct catgaaggag ttgctcaagc ccatactgat cccttgctcg agggtggttc
     8821 tcttcgatgt cagcgcagta atggctggtg tcagcaaggc ggttgccagt gtgaagaacc
     8881 caatggcgat cagaaaggtt acaaaggtgt tccccaggga gatgccgatg aaaccaagag
     8941 cggtggcgag cagagtggtc ttgatgacgg cggcctctcc ccagcgcctg gtcagcgggc
     9001 cggtaaagac gccctgagcc agggcggaaa ccatccccac aaccatcatg atcgccccaa
     9061 cctcctgggt gccatagctg aacttctgca atgcatacag accgaatatg ctgtagaaag
     9121 tagtcatggc gtatgagacc aggaaggcca tccccagcag gatgccgatt gggccaacca
     9181 tggcttgcca gagatcactg gcggtgaggc gctttttgtg actggcctgc cggcgagctt
     9241 ctaccgacaa tgactctggc agaaacagcc aaatcaaaag cagggccagg aaagacatcc
     9301 cgccggcgat gaaaaacggt atggacagtg attctccgcc cagtaaaccg ccaaagcctg
     9361 gtcccaggat ggtgccgatc ccaatggcgg cgcccagcgt gcccatcccc ccaccgcgat
     9421 ccttctccga tgtgctgtcg ctaatataag ccattgtggt tggtgaggtg gcagacgaga
     9481 gaatacccga gagtgagcgg aagacgaaca gcatccacag ttttgtcgcc aatccgaaca
     9541 gaatcatggt aatcccatag ccgaagatac ccaccatcag gatcggtttg cgtcccacgc
     9601 ggtcagaaag gctaccccag ataggcccga agatcaggcg catgatggcg tagctggcaa
     9661 ccagcaggcc tagctcgatg ccgcccgcac ccatgtcctc gatgaaaaaa gggatgatgg
     9721 ggatgacgac accgaacccc aacataacga ccaggagcac aaatgccagg atgtatattt
     9781 ggcgacgacc tgatttggct gaattaacca tcatgtacct ttacgagctt atatggatgc
     9841 gcctgatcta ctcgtgaccg tttagcagcg tcactgtccc agcgtcgcca tccactgcga
     9901 tcttctgacc gctggagatg cggtgcgtag ccacgcccgt accaagaacg gcagggatgc
     9961 cgtattcgcg agcgacgatg gaaccatggc tgagtggccc accgatgtcg gtgaccacag
    10021 cagaagccat tgcaaagagt ggcgtccagg caggggtggt tatcttggct actaagatgt
    10081 cccctgcttg catctggtca aaatcctgcg gcccgtgcag cacacgcgcg gtgccggtca
    10141 cacgtccagc gctggtagcc acgcccttca gggtatcgcc cacctgaccg atttctcctg
    10201 ccccgacaaa cgcttcgatg tcgaagccca tatatttctt ggttggcggc aacgtcggcg
    10261 gtggcaccag gagttttttg gatttccaga gcgctttgcg ctcttgcatg gaatcagcta
    10321 ggctgtttat cgcgcttcct tgctccatcg ccgcgatcag ggtttcaatt tctgcctgtt
    10381 ccagccagaa aatgtcatcg gcttgctgga tagcccctgc ttccacgagg cggctgccca
    10441 actcgaacag catctcccgt agcagcgggt atcccagacc gatatctgcg atgccgtcct
    10501 cgcgcacctc ggcctggcgt tgagccagct taagtgttgt gcggaaagcc caacggcgca
    10561 agcctttcag ccgcctaagt gtgttctcgc tggcttgcag gcgtcgctca gcggcgacct
    10621 gctggcgttc atgcgggttg gtgccttcgc cacgcagata catcttgatt gtctccagca
    10681 tggaggttgg gtcatctttt ggtaatggct tggcgaagtc cagatcgtag atcaggtgtc
    10741 caaaggcatc caggtaactt tggaagcgtt cctgccattc atgccaggta tctacattca
    10801 cgcttagggg tgcaatatta ctgtcgagcg cggcccggat ctgaagggta ggcgtctcgc
    10861 tgagataaac cgccaggtca gggatgttgc ggctccactt agccaggtcg tacagagtct
    10921 tttcagagcg gatggggatg ctatcgtacc ccatcaggaa ggctggcgcg gataggtcgc
    10981 catctttctt gatcattttt tcgtagacgc gtgtaaacag catttcagtg ccagccgcca
    11041 cgcccatcgt cccaaccatt aacgagccga ggtgatcgat cgcagcctcc ataacctcat
    11101 gcaccccgga gaaaatctcg cttggggtaa gactcaccaa aggcttatcc tcccagcgct
    11161 ctacggtttt cagataatat ggcctcacct cgtcgcgcca acgcgggatg ccgctgcgca
    11221 gcaagctcgg gaattttggc accaagacaa aaataatcca gagcatttgc ctcggtgtgt
    11281 atttggcggc aaagtatgca taatcattaa tggtcagtat cgaatcatct ggccagtttg
    11341 cgtcttgagt ttccttaccg accatataag cgatcaggcg cttaatgcca acattgagct
    11401 ctgaaacccc taatgtagca aagagcggcg tcaggggatc gggcaaaaag tcgatgatgc
    11461 tggtacgcat gtactgacct tttggatcag gcaaggtcca ttcaaccgag gctaaggtct
    11521 cgctggtcgg cagggctgtg ataggccgtg cctggacgat ggagaattcc ccatccgtcc
    11581 atgtccactc gatgtccatc ggcatgccgt acaggtcctc tatctggacc gccaggcggg
    11641 taagctcagt tgctatttcg tcatcaagaa cagggatgcg cctcaggttc tctgggatag
    11701 gctgttcctc ggttttccca tccacacgca cggtcatcac ctgcttatcg gcggtttggc
    11761 gctccagcac attcccggag gctttgtcca tgatcagcga gtcaggtgtg accagccctc
    11821 cgacaactgc ttcccccaac ccccaggagg cgctgatcag gacctgatcg cgccgtccat
    11881 tcataggatt ggcagtgaac aggatcccag cagcttcggc tgggacgagc agctgcacaa
    11941 ccactgctaa ggccacgccc tctgcatgaa tcccctgccg ggctcggtag ccgatggcgc
    12001 gcgctgtcca taggctggcc cagcacttct tggtcgcctc tagcacggcc cccgctccac
    12061 tgacgttgag ataggtctct tgctgcccgg caaaagaggc atcggggagg tcttcagcgg
    12121 tcgccgacga acgcaccgcc actgccgggt tggtgccggg aagctcagca tatgactgga
    12181 caatatcgtt ggcgatttct ggaggaattt gtccctggag gaacgcttcc cggatggtat
    12241 gggatgcctt ctcaagagta tttggtatgg atatatccac ttcttctaaa gctgccagga
    12301 tgcgcgtttg caaatcattt gcagacacga actggcgata agcctgtgtg gtcacgtgga
    12361 atcccccggg cacagggaaa cctgcgccag cgagccgggc tagcgaggcg cccttgccgc
    12421 caacagtttc caggcttgct tctgcatcgg taaaagaaag tacatagggt gtcatggttc
    12481 tctccattct aaagcgaaca atggtcgctc taatagacaa aaaaagaggt taggcttgaa
    12541 tgccattcat cacgatatct aatgcatgtg agagcatctg ttccggcgaa ccaaccgggc
    12601 gtgaagtgaa aacgaccggc gtcatcagcg caatcagtat tttggctacc atggctggat
    12661 ccacctgccg aatgctgcct tgtgcaacac cttgctcaat gatgctgcgc agcaggtcct
    12721 ggtaggcgta tcgcttgagt tggatctttt tttggctggc tgggctgagg cgctgaattt
    12781 ctaacatgag ctgtaacaag aaattcttgt tttttactaa ataatccagg tgtatttgca
    12841 tgagctgtcg gagcctctcg acggcagagc catcctggct ggcaatatcc agtgcaatat
    12901 ggttaatgtg gttcagttgt tcttcaacca caaacacgag caaatcctct ttagtgtgga
    12961 agtagtcata cagggtggat ttcccaacgc ctgcttcgat ggcgatctcg cgcatcgagg
    13021 tctccgtgaa acctttctct agaatgaggt gaattgtagc gtcgaatatt tcacggcgcc
    13081 gtctggcgtg ttcttcttcg gtgagaggaa ttcccttagg catcaataac tcctgaaagc
    13141 gaccatcggt cgctattatt ttatggcaaa gtcgaaattt tgtcaatacc tgaattgaaa
    13201 tggattttga atagacctgt taattacaac cgtcgcagca ctattagcct aataaagccg
    13261 cgtcctctgc ccttgccacc cgctgccctt cttctacatt caccctggtc aagagatagc
    13321 ctcccaggat gaagaatacc acgatgctga gcaccgccgg gcgactgctg ccaaagttgg
    13381 ccacagcaaa ggcgaatacc agcgggccga tgatggccga gaacttctcc atgacgccga
    13441 acaacccgaa gaactctccg cttttagagg ctggcgacat ggtcgaaaaa aggctgcggc
    13501 tgagcgcctg gctgccgccc tgaacgacag acaccatcca ggctaggaac cagaactcga
    13561 tgaccgagtc caggaagaag ccccagacgg agatcacggc atagaccgac agggcgagca
    13621 ggatagcccg acgggtgtca atggtcgccg ccaagcggga gaagaggggc ggacctaaaa
    13681 cccaggaaat cagcagtccg attatctcga ccgctgcgat caggccaatg accaccaaaa
    13741 ggttgctttg acgagagggt ggtaacacct caatttgggc caggcttatt tgtgtacctt
    13801 ggctctccgg agcttttcta ccggtattgg ccagcaccag ggtatgctcg cccggcgcgg
    13861 ctgctgcaag tactacctct tcgccatagc gcactgtcgg gtggtaggcg tctattgtca
    13921 ggggctcccc gctgtctggg tcgatcagcg gctgcccatc cattagaacc gcccagattc
    13981 cgccctgagg accactgctg taagtgagtt cgaccttttc gccgttgaag gtgaagctgg
    14041 ctcttgcctc tgcgtcagac ccggcgcggt aagtggcgct ggtctcacct cccagctccg
    14101 ctgcgctcac ttctaggtca ctccatgtgc cggtcgtctt caggcggttg tcggtggcta
    14161 agtaaatacc ctgtccggcg gctgttgggc tgctggcaaa gcgggcaggt ggcgtgccgg
    14221 tgatctggga tggcaggagg cgcgtgccag cgatccctac caagggcagg gcaaccaggt
    14281 tgaaaagcac atatgccagg aaaaacggcc gacgtttctc gttttgactg ggtagccggc
    14341 caaagatcag gctgtagggg atgcccacaa attgcaccag cagcagcgcc aggatcagct
    14401 ccaggctgcc aaagcccagc tcggcgccat agatagctgc tacgccaatg atagtgccga
    14461 tgccatcatt gtaaattaag aagcccagta aaaacttaaa cagctcgcgg tattggcgga
    14521 tgtctctcag tgtctctgcc aagcgcttga agctgactga aatcacattt tcacctgagt
    14581 ccagcttggc tgtggcggct ggcggctcag gcacgcgcag gaaaagcggg atagagaaga
    14641 ctgcccacca aatcgccacg ctgaagaagg acaggcgcgc tccccaggtg ccgggcagca
    14701 tctggatcat gacaatgttg attgccagca acagcccgcc gcccagataa cccatggcgt
    14761 agccgcgcgc tgacaccttg tcccggtctg cctcgcgcgc cacatgaggt agtaaggcat
    14821 catagaacgt gttggctccg ctaaaaccga tgcgccccaa gacgccgaag acggaggcta
    14881 atacccaatc ccccgtcccg accagtatca gcaacgcagt gctgacgatc ccgattccgg
    14941 cgaaaaccgc caggaaaggc ttcttaccgc gcatcacatc gctgatggtg cccaggatgg
    15001 gcgagagcac agcgatgatc agcagggaaa cactcagccc agtgctccag tacgctgtgg
    15061 cggtggccgc gctgggcagg ttcgcgccgg cgacttggct gaaatagatg ggtagcacag
    15121 cagccagaat cgtggttgcg aaagcggagt tggcccagtc gtacattgtc caggcccaaa
    15181 tacgccgaga gtattctttt tcgtcaattt ggattagggc agaggtactg gcagccatcg
    15241 tggaatctcc ctataggtca gatgccagca attatagccg acactgcaat aatacattga
    15301 tactactcac ccccaatttt ttagagaaga agggctggaa aaacgcaatc attgtctttt
    15361 ttggaaattc ccccaataat aagcccaata gataagtttt ttgaattata atgagacaca
    15421 tattaattca tttggcgatc cactccggcg ataattattc tctttgatta ttctttacct
    15481 gcgcgaagat gccggattgg ttgctgcatt attcaaggac ttatttttta ttatctacag
    15541 taagtagctt gataatagcg accttgggtt gtcacgccta ttgtgacagg cggacaggtg
    15601 tgagcgcagg tatattgcgc tctttttgtt tttataagga gttattatga gcgttgaatt
    15661 tatctttcgc ctggtcggta tggtagtgtt tgcaatcatg ggtgcttatt ttggggtata
    15721 tctggcggcg atctcgaatg gaccgctgga tatgtatggc gtggtggtgg cgttaaccgg
    15781 agcgttggca ggtctgatct tgacaccata cttgaccacg cgtccggcgc gatatatgcg
    15841 ggcagtgttc acgcgcatct ccgcgcagac tctggcggca agtctgacgg ggttaattgc
    15901 agggttgatc attgctgcct tgctggcttt ccctttctca atgctacctg acccatttcg
    15961 tgctatcctg ccatttatcg gcgtgctcgt ttttggctat ctcggtgtag cggtatttgt
    16021 catgcgccag aatgatatct tctcgattct gaggatcaac cttcctgggc gctctgcgcc
    16081 aggaaatgat agcggcgcaa atcgtgatgc ctcacgcacc attttgttag ataccagcgt
    16141 gattatcgac ggtcgtattg ctgatatcac ccgcacaggt ttcctggtag gcacgcttat
    16201 gatcccgaga tttgtgctca atgaattgca atacatcgct gactcagccg acggtttgcg
    16261 taggcagcga ggccggcgtg ggattgaggt gctatctcaa ttgcagaaag acacaacgat
    16321 ccccgttcgc atcagcgata ttgatatcga aggaacgcga gaggtggatg ataagctggt
    16381 catccttgca cgccaattgc gttgtcctat cttaaccaat gactataatc taaatcgtgt
    16441 ggctgaactg caaggtgttc cggtgctgaa tgttaacgaa ttggcaaatg cagtgaaatc
    16501 ggtgctgctg ccaggcgaag cgctggcggt gaatatcatc caagaaggta aagagagcgg
    16561 gcaaggcgta ggttacctgg acgatggcac catggtggtg gtggaagatg ggcgcggtca
    16621 agtaaacagt aaagcgacgg tggtggtcac caaggttttg cagacggcag ctggtcgcat
    16681 gatctttgcc cgcttggacc agaggggtga actatagagc ggtttcacac tatcgctgaa
    16741 ggtgcattgg tagcacgctc taaaattaat tgactatgcg cgaatggatc aacgggcgaa
    16801 acccggttta cgaggtgctg cgcgccgcga ggcgacaagt tttccgtctg caaattgccc
    16861 agggtgtcca ggaaaagggt cgcctggctg agatcgcgag c
//
LOCUS       DBNGSX010000055         4837 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1319171, whole genome shotgun sequence.
ACCESSION   DBNGSX010000055 DBNGSX010000000
VERSION     DBNGSX010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1319171"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="AC16CB_01755"
     CDS             complement(<1..808)
                     /locus_tag="AC16CB_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HIR3341220.1"
                     /translation="MTIIEAEIRSGVYYDSVILMQLQRSLIELPGILDAGVVMGTPAN
                     KDILTQSNLLFPEVEAAVADDLVIVVKGSDRESAKAAINKVDELLVTRRGTETEHEYR
                     PKSLDTAIQLLPEAQWVMISVPGRYAARIARQSLNSGKNVFVFSDNVLLDDEIEFKKL
                     AAAKGLLVMGPDCGTAIINGVGLGFANKVRRGSIGIVAASGTGLQQVSARIHQFGGGI
                     THAIGTGGRDLSSDVNAVTARQGLELLSRDANTQVIVLVSKPPSASVADEL"
     gene            complement(877..2085)
                     /locus_tag="AC16CB_01760"
     CDS             complement(877..2085)
                     /locus_tag="AC16CB_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3341221.1"
                     /translation="MRPLKDLRVLDVSRALAGPYCTMIFGDLGADVIKVELPGRGDES
                     RNWGPPFVGEPYEVYPGESSYFMAINRNKRSITVNLKTAEGQEIVKRLAAVSDVFVEN
                     YRTGTLEKMGLGYEDLHDLNPRLIYCSISGYGRNGPYAERPGYDFIIQAEGGIMGVTG
                     PEDGSPYRVGISIIDITTGIFASTAILAALHARELSGEGQLIDISLLDTSVALLSNVA
                     ANYLVAGVEPKRMGNAHFNIAPYEVFRARDKWFTLGAANVRQWEMLCEVVGKPDLKND
                     PRFTTNKDRVANRGALSKILNDAFAQQDAEFWLSKLQEAEIPSGPINTIADVFDHPQA
                     MARDFSVQIDHPTAGSINLSGFPYKLSQTPPEMYRHPPRLGEHTSEVLAKVLGYTVEN
                     IEHLKEQKVI"
     gene            complement(2449..>4837)
                     /locus_tag="AC16CB_01765"
     CDS             complement(2449..>4837)
                     /locus_tag="AC16CB_01765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3341222.1"
                     /translation="RRGPHEQEGLMTTLPTSEKVPHPIGDSVPRVDAHEKVIGSALFA
                     DDLQFGPGLLYARILRSPHPHALIKRIDTSQARALPGVRAVVTGEDYPGYIGLYLQDR
                     HIFCRGRARYVGDPVAGVAANSEEIAEKALELIEVEYELLPGVFDPEFGASPDAPLLH
                     PDLGIYEVANFIFPEPGTNISNHFRIRKGDVEAAWLECEAIVERSYRIPHVQHVPIEP
                     HVATAKVDEQGRVTLWGSSQSPFAQRNLIAKSLGKSQSDVQVVAPYVGGGFGSKAGVS
                     MEALAVAIAMKVPGRPVKLRLTREEEFYTAFVRQSLVSHFKMGCDKEGRLLAMEVKFY
                     WDGGAYTEYGVNITRASGYSSSGPYEVPNIKTDSYCVYTNHPVSGPMRGFGMPEMHSG
                     LEQCIDELAHAIKMDPVQFRKINCVKKGSTLITGMVMHPTGLSECLTKAAEAIGWGKK
                     DKPSAPNKRRGKGIALMWKAPAMPPNAGSSAKVALNEDGTLTVSVGGQEIGQGSFTIA
                     AQMAAGALGVPWDWVRVATPLNTDYSPYEWQTVASRLTWSMGNAIVAAARDARKQILD
                     MVAKAWSENPDDLDIIDGKVISYKSEESISLKDIVIYGISLPDDQGWIGGPVMGRGNF
                     MPTYVTGLDPETGQGPRAVVHYTTGAQAVEIEVDMDTGKVDVIRGVAAFDVGKAINPD
                     MVKAQMEGGFVQGLSTALFEDLKLKEGFLQNSSFVDYRIATSSDAPQSIEAIIVEVPQ
                     DDGPWGARGIGEHSMVPTIPAIANAIYDAIGIRPGAPPYTSEKIYLAMQDAGLVD"
BASE COUNT         1091 a         1337 c         1196 g         1213 t
ORIGIN      
        1 ctaattcatc cgccactgat gcagacgggg gcttggaaac caacacgata acttgtgtgt
       61 tcgcatccct gcttagtagc tctagcccct ggcgtgcagt tactgcattg acatcactgg
      121 atagatctcg accgcctgtc ccaatcgcgt gggtgatccc gcctccgaat tggtgaatgc
      181 gcgcgctcac ttgctgcaaa ccggtgcccg acgcggctac aattccaatc gatccacggc
      241 gcaccttgtt ggcaaatccc agaccgaccc cattgataat cgctgttcca cagtctggcc
      301 ccattaccag caaccctttg gcggcggcta gcttcttgaa ctcgatctca tcatccagta
      361 aaacgttatc gctaaaaaca aacacgtttt tgcctgaatt gagagactgg cgggctatac
      421 gtgccgcata gcgcccggga actgagatca tgacccactg cgcttcaggt agtaattgaa
      481 ttgctgtatc caggctcttt ggacgatatt catgctctgt ttcagttcca cggcgagtca
      541 cgagcaactc gtccacttta ttaattgctg ctttggcgga ttcccgatca ctgcctttta
      601 cgacgatgac cagatcatcc gcgacggcag cctcgacttc tggaaaaagc aggttgcttt
      661 gagtaagtat atctttgttc gcgggtgtcc ccattaccac cccggcatcg agtattcctg
      721 gaagctctat cagggaacgc tgcagctgca tcaagatgac tgaatcgtaa tatacaccag
      781 accgaatttc tgcttcaatt attgtcattg ttcaatccaa attgtaaccc tgtgttttga
      841 taagcatgga tagtgatttt tagcccgttt tatcggttaa atcaccttct gctcttttag
      901 gtgctcaata ttctcaacag tatatcctaa cacctttgcc agcacttcgc ttgtgtgctc
      961 tcccaatctt ggagggtgtc gatacatctc tgggggagtt tgcgatagct tgtacggaaa
     1021 accggacagg ttgatcgacc ccgccgtggg atggtctatc tgcactgaaa aatccctcgc
     1081 cattgcttgc ggatgatcga aaacatctgc gatggtgttg atcggtccgc ttgggatttc
     1141 agcttcttgc agcttgctca accaaaactc agcatcttgt tgagcgaaag catcgttcaa
     1201 tattttcgac aatgcaccac ggttggctac ccggtccttg ttggttgtaa accgcggatc
     1261 attttttagg tctggcttgc caactacttc gcatagcatc tcccattgtc gaacattcgc
     1321 cgcacccagg gtaaaccact tatcccgagc gcggaaaact tcataaggag caatgttgaa
     1381 gtgtgcattg cccatgcgct tcggttctac ccctgctacc aggtaatttg cagcgacatt
     1441 cgagaggagc gcaacggaag tatcgaggag cgagatatca attaactgac cttctccgga
     1501 taattcccgc gcgtgcaaag cggccaaaat cgccgtggag gcaaatatcc ccgtcgtaat
     1561 atcaatgatg gatatgccaa cacggtaggg agagccatct tccgggccgg taactcccat
     1621 aatcccgcct tctgcctgga taatgaagtc gtaaccaggt ctctcggcat aagggccatt
     1681 ccggccgtat ccactgattg agcaataaat caagcgtgga ttcaggtcat ggaggtcttc
     1741 ataacctaat cccatctttt ctaatgtgcc agttcgatag ttctccacaa acacatcgga
     1801 gacagctgcc aggcgcttga caatctcttg gccttcagca gtcttcaaat taaccgtgat
     1861 gcttcgttta ttgcgattga tcgccatgaa ataagacgat tcaccgggat aaacctcgta
     1921 tggctcaccg acaaagggcg gaccccaatt gcgactttcg tcgcctcgac ctggaagttc
     1981 caccttgatc acgtcagccc cgaggtcgcc aaagatcatc gtacagtatg gaccagctaa
     2041 agcacgtgaa acatccagta ccctcaaatc tttcaatgga cgcatgcaat ccttcccatg
     2101 atgaataccc ggattttttc aggtcttagg tcgttcatga taagaggttt ttcgctaact
     2161 ttaccgctac gtctttacac gtatgacatt aagacgggtt ggtctactgc gcggtgagct
     2221 cacccgtttg gtgtaatttt aaaaatatgg cctgtagaag aactgtgaga agggaaatcc
     2281 atcagtgtca taggcgaaat gtggatcagc gacattgtca atgggaatct aagatccaat
     2341 ttccgggttc attatacgca gaattggatg ggaaatcaat ctgtcttact tccacgttag
     2401 catcgtttgg tctggcggtc gttcctcact tggaaatcac gtcaatagtt aatccacaag
     2461 cccggcatcc tgcattgcca ggtaaatctt ttccgaggta tagggcgggg cgccggggcg
     2521 gatgccaatg gcatcgtaaa tggcgtttgc aattgccgga atcgttggaa ccatggaatg
     2581 ttcgccaatt ccgcgcgcgc cccacgggcc atcatcttgc gggacctcaa cgataatagc
     2641 ctcgatggat tgaggcgcat ctgagctggt agctatgcga taatctacaa aactggagtt
     2701 ctgcaagaag ccttctttga gctttaagtc ttcgaacagg gctgtactca gcccctggac
     2761 aaagccccct tccatctgtg ccttgaccat atccgggttg atagccttgc caacatcgaa
     2821 agctgctacc ccgcgaatca catccacttt tccggtgtcc atatccactt cgatctctac
     2881 tgcctgggct ccggttgtat agtggacaac ggctcgtggt ccctgtccgg tctcagggtc
     2941 aagtccagtc acataggtgg gcataaaatt gccacgtccc atgaccggac cgccgatcca
     3001 gccctgatca tctggcagtg agatgccata gatcacgatg tcttttagag agatggactc
     3061 ctcggattta taggagatca cctttccatc aatgatgtcg aggtcgtcgg ggttctccga
     3121 ccaggctttg gcgaccatat cgaggatctg tttgcgggcg tcgcgcgccg cggcaacgat
     3181 agcattgccc atgctccacg tcaggcggct ggcgacggtt tgccactcat aagggctgta
     3241 atcggtattg agcggagtag ccactcgcac ccagtcccac ggcaccccca gggcacctgc
     3301 tgccatctgg gcggcaatgg tgaacgaacc ttgtccaatc tcctgcccac ctacactgac
     3361 agtcagggtg ccatcctcgt ttaatgccac ttttgcactg gagccggcat tgggcggcat
     3421 ggcgggggct ttccacatta aggcaatgcc cttgccgcgt cgcttattcg gggcgctcgg
     3481 cttgtccttt ttgccccatc caatggcttc agctgccttt gtgaggcatt cactcaagcc
     3541 ggtggggtgc attaccatgc cggtgatgag cgtgctgccc ttcttgacgc aattgatttt
     3601 tcgaaattgt acagggtcca tcttaatagc atgcgccagc tcatcgatgc attgctccaa
     3661 accggaatgc atctcaggca tgccaaagcc gcgcatcggc ccgcttaccg ggtggttggt
     3721 atagacacag tagctgtctg ttttgatgtt cggcacctcg tagggtccgc tgctgctgta
     3781 accggaagcg cgggtgatgt tcactccgta ctcggtgtaa gcgcctccat cccagtagaa
     3841 ctttacctcc atcgccagca ggcgaccttc tttgtcacaa cccatcttga aatgagaaac
     3901 cagactctgc cgcacaaagg cagtgtagaa ttcctcttca cgtgtcaagc gcagtttcac
     3961 agggcgccct gggaccttca tggcgatggc aacagctaag gcttccatgc tgaccccagc
     4021 cttactgcca aaaccgccgc ccacatatgg ggcgaccacc tgcacatcag attgggattt
     4081 ccccaaagac ttggcgatca ggttgcgctg cgcgaaggga gactgagagc ttccccacaa
     4141 cgtcacccga ccttgttcat ccacctttgc tgtggcaaca tgcggctcga tcggtacgtg
     4201 ctgcacatgg gggatgcggt aacttcgctc gacgattgct tcgcactcaa gccaggctgc
     4261 ttccacgtct cccttgcgga tgcggaaatg gttggagata ttagtgcctg gttctgggaa
     4321 aatgaaattg gcaacttcgt atatgcccaa atcagggtgt agcagtggtg cgtccgggct
     4381 ggcaccaaac tccggatcga acacgccggg taaaagctcg tattccactt cgattaattc
     4441 aagcgctttc tctgcgatct cttcgctatt cgccgccaca ccagccaccg gatcgcctac
     4501 gtagcgcgca cgacctcggc aaaaaatatg gcgatcttgc aggtacaaac caatatatcc
     4561 tgggtaatct tcgccggtaa caaccgccct tactcctggc agggcgcgcg cctggctggt
     4621 gtcaatgcgc ttgatcagag cgtgagggtg tgggctgcgc aaaatgcggg cataaagcag
     4681 tccaggacca aactgcaggt cgtcggcaaa aagggctgag ccaataacct tctcgtgcgc
     4741 atcaacacgt gggacgctat ccccaatggg atgcggtact ttttcggaag tcggtagtgt
     4801 ggtcatcaat ccctcctgct catgtggtcc acgcctg
//
LOCUS       DBNGSX010000056        12069 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_280481, whole genome shotgun sequence.
ACCESSION   DBNGSX010000056 DBNGSX010000000
VERSION     DBNGSX010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12069)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12069)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12069
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_280481"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..102)
                     /locus_tag="AC16CB_01770"
     tRNA            complement(20..102)
                     /locus_tag="AC16CB_01770"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67..69),aa:Leu,seq:taa)
     gene            252..767
                     /locus_tag="AC16CB_01775"
     CDS             252..767
                     /locus_tag="AC16CB_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341223.1"
                     /translation="MGSQELLRRMIGMMLIALPLVGCASPASNPTPVLPIATPTAVPP
                     TATPSLIPPTITSTPDYIKVISLDDILGTWYNPNKKLYLRFYEDGILHHSHLLDVLDA
                     DPFAKTEIWFEGGQLYIKTKEVHGVPDCGDAIGVYEVRLYPKGNIQIVKIEDSCSPRS
                     GDTALPYNPVR"
     gene            916..2640
                     /locus_tag="AC16CB_01780"
     CDS             916..2640
                     /locus_tag="AC16CB_01780"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR3341224.1"
                     /translation="MVNKLYLPLCLLFLCVALSACVAKPDVQMSYDPGALRFSGDRAM
                     ELERDFVTRFPSRHSGQSNNRLAVKWLSAKLTDLGWSCSIDEWEIINYSRPVALNNAV
                     CRLPGKSDREILVVAHHDQAPTTIQGADNNGSGVAILLHLAEVFANENPLPYTLVFAF
                     TDAEEYGMIGSRRYVETHPNLQQIIAGISLDNLGRTYYDGVKMELVGRYDGYGALWLA
                     LTAREAARAAGLWDVYLRSTFDQVTDQAAPVSLMDQGPLVAASIPAIGFAGHFPESYA
                     DENYRLWHDPDDTLDYQSANVLGKSGLTAEALIRQLLSMDKFPKAFNPYLYFDSSGQV
                     LQGLPLWLIFCGFVIIFFLGSLLGGKTSIQEKLLGWRFALPHFLGLWMPLVASIILLY
                     VFVWVGLMDRYHAYPATTKDPALLHPRWPAVILFLLGLGVFFYLGRRLVRDYVNSTQH
                     ATHIQIKSLSMFIIGLSGVYVLWLNPFALLFFVPLLFWLLIAGRRGVGKILDLLLFLM
                     GGLVVYALIYVFGFITLRYNLAFLWFMMNMFSIRMISFPTAAMITAIFGAGLALVVNP
                     AIDRGRSK"
     gene            2637..3644
                     /locus_tag="AC16CB_01785"
     CDS             2637..3644
                     /locus_tag="AC16CB_01785"
                     /inference="COORDINATES: protein motif:HMM:NF039685.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6206 family protein"
                     /protein_id="HIR3341225.1"
                     /translation="MRGYEIEHYASLAMCQVDFELLKEFENGLNIASPKHSYTPCKVL
                     GYGEISTVLQIQDDSMRNLAFKRMSIFDSTEELRKYLELYLEYTRMLEYDLGMCLLPH
                     GHAAFKNASGRQIFYIIQLEVPPASIANKAIHYLSPAETKLLIRAILRELHKVWEFNL
                     DNQERQIAIDGQISNWSIANFDEDNPRLEEKFTLHYFDTSTPFLRIQGVEQLNPELFL
                     RAAPSFLVWILRLLFLEDVMTRYYDARKVTIDLLANLYKEQRSDLIPDMVAAINDFFS
                     GEAAHLGVKPLQQSEISSYYREDRIIWSWYLSMRKFDRFLQRVILRREYPYILPEKIK
                     R"
     gene            3673..4920
                     /locus_tag="AC16CB_01790"
     CDS             3673..4920
                     /locus_tag="AC16CB_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010879310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR3341226.1"
                     /translation="MSIIGHESKEGITDIFARHVSSGKAAFFAQAGIDFIIGQRDGIY
                     VWDLDGKRLINCHCNGGVFNLGHRNPRVVAALQRALGELDIGNHHLISEARAILAQRL
                     AAISPGDINRVIFGVSGGEAIDMAIKLARGHTRRSKIISAVGGYHGHTGLALAAGEEM
                     YRKPFQPLSPGFIHVPFGDLDALEATMDGDTAAVIFETVPATLGIVLAPENFFSGVRE
                     LCDRYGAVMIVDEVQTGLGRCGQVWGIDTYGVIPDIIVTGKGLSGGIYPITATMYREH
                     LNPFMHDNPFIHVSTSGGAEIGCHVALEVLDVLEEPGFLEHVRSLAFQFKEGFARLQD
                     NHPVIMVEPRQRGLMMGLKLANADYGPIMTQLGFHAGLLTIFANHDPSVIQILPPLII
                     QPGEVEQVLNILDEMLGMLEMMV"
     gene            5100..6671
                     /locus_tag="AC16CB_01795"
     CDS             5100..6671
                     /locus_tag="AC16CB_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341227.1"
                     /translation="MDDAYIHMVYAQNLVDKGSLMFNDPEEKGVGTTSASWVLLLAAG
                     YRLGISLHVVLKILGVASLIGLGVGLYLLLVRYWAAVPALIATLFVILSGHMLWFALS
                     GMETILFLTIGVFALLFYRNERWIWLGIFLGLLTLTRPEGILLAVSIGLVDIWRRKAL
                     AKGLVMAGIVVFLMCAPWYGYILARTGNLLPTSGIGKRVSSQVGMHLLLQQNEALSVF
                     AKYPSLLYPFVWLGYLLEYVLGGVALPPPDLRLVTSIASETYSISAWALLGFVGVVAP
                     LVWRGSRQIFSGLRQPNSILGEKSALMLVCLIWVVLHNLSYMSFLPFPGTASRYGAMN
                     HIVLWIVLLSGLFGFWHRRGIFLWLAAGLSVIGVANMGYWNRVYDANIDHMVSVRIQA
                     ARYINRVLAEEELCAAMDVGALRFYSQRPIVDLGGLIDPELGKLFLSGDLDRHLVEMG
                     VTCLILPGRAGESTDGWFDIAKELGLLESPFVRLHQEKVFEMDRKRWLFGYQPTRNYQ
                     ATVTIYRMESLCSMC"
     gene            complement(6675..7364)
                     /locus_tag="AC16CB_01800"
     CDS             complement(6675..7364)
                     /locus_tag="AC16CB_01800"
                     /inference="COORDINATES: protein motif:HMM:NF014826.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR3341228.1"
                     /translation="MTPTQIPMLRVEFHSHTMYSKDSLARPERLLSACRRKGIDRLVV
                     TDHNTIAGAMQCQELDPERVIIGEEILTQSGEILAAFVQEHIPARLTAQETIAHLRAQ
                     GAFISVSHPFDITRKGHWQLAQLLEIAPLVDAIETFNARCFLPSFNTQAQEFAHQQML
                     LGTAGSDAHTTYELGRATMLMPDFHDAESFKTALFQAQFETSLSSPWIHLSSRYATWV
                     KKMQRKRSQNR"
     gene            complement(7411..7737)
                     /locus_tag="AC16CB_01805"
     CDS             complement(7411..7737)
                     /locus_tag="AC16CB_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341229.1"
                     /translation="MLYILSGVAVVVLMEWFLITAWLSRSQTAWQYNFWAFVIVLLWA
                     QPWSSRNPYRTGRQIIRNMVYLVLLLATLASAIVFKRWGPLLVTMEILILALIEFAGR
                     RSLKAE"
     gene            7918..9453
                     /locus_tag="AC16CB_01810"
     CDS             7918..9453
                     /locus_tag="AC16CB_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341230.1"
                     /translation="MDQRLAKQAYKVQIILVSLFVILLYLYLANRASPDFSLWELIKG
                     VVKFFLLMWDRIPEGVMVFFRDMVLVVAAFLFWIAFFAQFALPVRNFGQRILASNYLF
                     SYAIGRHGPAIKIDNGDIPDRYPRDKPHRRGVIVLDTASAAVLRTRTQFTRSVGPGLV
                     FTLPGEYLAGSVDLHQKISHVPALGPLADEMYPFAPWDQENEAEEDHEERQKRRFETS
                     GETRDAVEVVPNIHTVTLIDPDHRPNQLSGVPADRGLYSWLREYMQSDSHTRFGYNPE
                     AVRLIVTGEAVDPMVNMPIPEKRQIPWYQFPAYLAVDLWREYLRRFTFEELFSRLEDY
                     GGQTAFQVIQDKIFERLTRPYVLEINQVGKPTGRLVESREFAMLRERGIRVLAAPIRN
                     LRFEKKIDQQLEDQWVSFWRLRAEAERDYINRRRSYSIHEGEKDAQREFAFWATRRIE
                     SSLLQSPKPPSQAEARIQMRVVLERLLRGTLGQCLHDTRLHQRLSGEESQLITIIEWL
                     RRL"
     gene            9462..11243
                     /locus_tag="AC16CB_01815"
     CDS             9462..11243
                     /locus_tag="AC16CB_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341231.1"
                     /translation="MNRNRKPRTMKYELSLNAMITDWLRWLAPLKDPVFGALDLLTAW
                     FFDFGSGWAILRWFAITLVGAGLWVLLAIQPLEKLAGAIDPNLVFDQVFYVICQVRET
                     IVALPPEEAAVHFCGAYANPEAILFEPLIFEIPLQWLFGAGVFRHILLLAFACWLAFK
                     IASQYQADVYDIPDISCSEHFLMQAALINPRNVINIQNASQRPLEEHLPLYLIGGPGK
                     VCVHLENAALFEKMDGVPRVVGPTVRQPVTLDGFERLRKVIDLRDQKESFDIRGRSQD
                     GIRVAAKDVQVVYSVFRNHIEPSYEQPYPFQDPRAIENLIFQQSQQVWNLSVGIQIRG
                     ALLDFFAQHTLNEFLAMIQEPELKKAQEYELELMKESRRLSGSEEPIGTPPIENVDGI
                     EYVSRPQITDLFYERAEETKLARGAEIDWVGVGTWDFPAQLIPVRHQEAWRITHDNLM
                     KNNPAVFNGLRWQYKINETLRLIQMVPIATFKKMISTPDIHTDDAIRALILAYHTQMR
                     EAYADYERSERELEKLESQETDPVKVLEHREKLGKLIAEKQRISEVMAFLARFIGRWL
                     VGPSLTGPTPKGPLPTGPQAAESGNGG"
     gene            11248..>12069
                     /locus_tag="AC16CB_01820"
     CDS             11248..>12069
                     /locus_tag="AC16CB_01820"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR3341232.1"
                     /translation="MFDSISPNPDGPVLVIGAAGVDIVGRLRGELRSKTSTPAVIRSS
                     YGGVARNVAENLVRLGQPVNLISVVGEDDEGDRLLQGLIAAGVNPERVLRTSQYVTNS
                     YLAIIDSQGELQFALDDMRAINSLTPQFIREQAALFKEASILFVDANLPKDTLRTVMS
                     LARRAHLPVCADPTSVSLAARLRPHLSRFFLTIPNNAEAAVLSERTSPVNGRRQALEV
                     AKYLVSQGVDIVIITMAEQGLCYATSESSGYIPAIKTSILDPTGAGDALTATVIFA"
BASE COUNT         2729 a         2861 c         3249 g         3230 t
ORIGIN      
        1 atccgcttaa aataaaaaat ggggcgggtg ggagtcgaac ccacacgagg ttaccctcag
       61 tggattttaa gtccacggcg tctgccattc cgccaccgcc ccagtggggt taattgtaca
      121 tcgaatgggg tatgggtcaa gatgtttatc tgtttatcct gagaactcta atttgactta
      181 tgccagtttc gagtatataa catgagtacg aggccaacgg cagccccgct ttcgttacga
      241 agggtatgaa aatgggttcc caagaattgc taaggcgaat gattggcatg atgttgatcg
      301 cattaccttt ggtgggatgt gcttcccctg cttctaaccc tactcctgtc ctgccaatag
      361 caacacccac agccgtcccg ccgacggcta cccctagcct catccctccg actattacct
      421 ccaccccaga ttacatcaag gtaatttccc tcgatgatat cctcggtact tggtataacc
      481 cgaataagaa actgtatctc cgcttctatg aggatggaat cttgcatcat tcacatttgc
      541 ttgatgtgct tgacgctgat ccatttgcca aaaccgaaat ctggtttgaa gggggacaat
      601 tatatataaa gaccaaggag gtccatggtg tccctgattg tggggatgca attggagttt
      661 acgaggtgag gttatatccg aaggggaata tccagattgt gaaaatcgaa gatagctgtt
      721 cgcccagatc gggtgatacg gcactgccat ataaccccgt tcgctgattt atgcgttaat
      781 aggttgagtg gtctgcactt agcgatcatt attcaacaca ctcaaattat taattagatg
      841 gcggactttg ctaataccag ttaatgtgcg cgattcgact gcataaccat tcttgatggg
      901 ctaggaggct taccgatggt taacaaactc tatttgcctc tgtgtttgtt gttcctttgt
      961 gttgcgctct cggcttgtgt cgctaaacct gacgtccaga tgagttatga cccgggagcg
     1021 ttgcgcttct ctggcgatag ggctatggag ttagagagag atttcgtcac ccgtttccca
     1081 tcccggcaca gcggacaatc aaataatcgc ctggctgtga agtggttgag tgcaaaacta
     1141 accgatttgg gttggagctg cagcatcgat gagtgggaaa ttatcaacta cagccggccg
     1201 gtggcgttaa ataatgccgt ctgtcgattg ccgggaaaat cagatcggga gattctggtt
     1261 gttgcccacc atgaccaagc gccaaccacc atccaggggg cggataacaa tggttccggc
     1321 gtagcgatac ttctccacct ggcggaggtt tttgccaacg agaatccctt gccctacacg
     1381 cttgtatttg ccttcaccga tgcagaagag tatggcatga tcggcagccg gcgctatgtc
     1441 gagacccatc caaacctaca gcaaattatc gcaggtatct cgctggataa cctgggaagg
     1501 acctattacg acggtgtgaa aatggagctc gtcgggcgat atgacggcta tggcgccctc
     1561 tggctggcat tgaccgcccg agaagcagcg agggcggctg gcttgtggga cgtgtatctg
     1621 cgatctacct tcgaccaggt gacggaccag gcagcaccgg tttcgttaat ggatcagggt
     1681 cccctggttg ccgcgagtat tccagctatt ggcttcgcgg gacatttccc ggaatcgtac
     1741 gcggatgaaa attaccgatt atggcacgac ccagatgata cattggacta tcagtccgct
     1801 aacgtattgg gtaaatccgg attaaccgcc gaagcgttaa tccgtcaatt gctttcgatg
     1861 gataagttcc ccaaagcatt taacccatat ttatattttg actctagcgg acaggtgttg
     1921 caaggcctgc ccttgtggtt gatcttctgt ggatttgtga tcatcttttt tctcgggagt
     1981 ttgttgggag ggaaaacttc aattcaggaa aagctcctgg gttggcgctt cgcccttccc
     2041 catttcttag gattgtggat gcctctcgtg gcttccatca ttcttctgta tgtgtttgtg
     2101 tgggtggggt taatggatcg ctaccatgct tatcccgcca cgaccaagga cccggcgttg
     2161 ctccatccgc gctggccagc agtgattcta ttcctcctgg gtctgggtgt attcttctac
     2221 ttggggcgga ggcttgttcg agattatgta aattcaacgc agcacgccac gcatattcag
     2281 attaagagcc tgtccatgtt catcatcggc ctatcgggag tgtacgtatt atggcttaat
     2341 ccattcgccc tgctcttttt tgtccccttg ctcttctggt tgctgattgc tggcagaaga
     2401 ggggtgggca aaatattaga tcttctgctt ttcttaatgg gtggattggt tgtttatgcc
     2461 ttgatctatg tgttcggctt tatcaccctg aggtataacc tggcatttct ctggtttatg
     2521 atgaacatgt tctctataag gatgatcagt ttcccaaccg cagccatgat taccgccatc
     2581 tttggtgctg ggttggcact ggtggtaaat ccagcaatag atagagggag gtcaaaatga
     2641 ggggatatga gatcgaacac tacgccagcc tggccatgtg ccaggttgac tttgaactgc
     2701 taaaggaatt tgaaaacggc ctgaatatcg cctcgcctaa acacagctac accccgtgca
     2761 aggttctggg ttatggcgaa atcagcactg tgctccaaat ccaagacgac tcaatgcgaa
     2821 acctggcatt taagcgcatg tcgatatttg attcaacaga agagttacga aaatatcttg
     2881 aactttatct ggaatatacc cgcatgctgg aatatgatct cggtatgtgt ctccttccgc
     2941 atggtcatgc agccttcaag aatgcatcag gtcgtcaaat cttttacatc atccaattgg
     3001 aggttccacc cgcctcaatc gcaaataagg ctattcatta tctctcaccc gctgagacga
     3061 agctcttaat tcgggcgatt ctgcgggagc tgcacaaagt ctgggaattc aacctggata
     3121 accaggaacg gcaaattgcc attgatggtc agatctcaaa ctggtcgata gcaaattttg
     3181 atgaggataa tcctcgcctg gaagagaaat ttaccctgca ttatttcgat acaagcacgc
     3241 ccttcttgag aatccaaggg gtcgagcagt tgaacccaga gttattcctt cgggctgccc
     3301 cctcattcct ggtatggatt ctcaggcttc tattcctgga agatgtcatg acacgctact
     3361 acgatgcccg caaggtaact attgatcttc tcgccaatct gtataaggag cagcgctctg
     3421 acttgatacc ggatatggtt gccgccatca acgatttctt ctctggcgag gcagcccacc
     3481 tgggagtgaa accccttcag caaagtgaaa tcagctctta ttatcgggag gatcgcatca
     3541 tttggtcttg gtatttatcc atgcggaaat ttgaccgctt tttgcagcgg gttatcttgc
     3601 gtcgcgaata tccatacatt cttcctgaga aaatcaagcg atgagatgcc ccgatgatcg
     3661 gaaggaacta tcatgtcgat aataggacac gaatccaaag aggggatcac tgatatattt
     3721 gcccgccacg tatcgagcgg caaggcagcc tttttcgccc aggcgggaat tgatttcatt
     3781 atcggtcagc gcgatggcat ctatgtctgg gatttagatg gcaaacggct gatcaattgt
     3841 cattgtaatg gcggggtgtt taatttagga catagaaacc caagggttgt ggctgcattg
     3901 cagcgcgccc tcggagagct tgacattggc aatcaccatt tgattagcga ggcgcgcgct
     3961 atactggcgc agcggttggc tgccatatct ccaggggata tcaatcgcgt gatctttggc
     4021 gtatccgggg gtgaggctat cgacatggcg atcaagcttg cccgtggaca cacccgtcgc
     4081 tcaaaaatca tctccgcagt aggcggatac catggtcata ccggcctggc tctggcggcg
     4141 ggggaggaga tgtaccgcaa gccatttcag ccactctcgc cgggtttcat tcatgtgcct
     4201 tttggtgatc tggatgctct cgaagcgacc atggatggcg ataccgctgc ggtgatcttc
     4261 gagacggtcc cagccacgtt gggtattgtg cttgccccag agaatttttt ctctggggtt
     4321 agggagttgt gtgaccgata cggtgctgtg atgatcgtgg acgaggtcca gaccgggctc
     4381 ggtcgctgcg gtcaagtctg ggggatcgat acctacgggg tgatacccga tatcattgtg
     4441 acaggcaaag gattatcagg gggcatttac ccgataaccg ccaccatgta ccgggagcat
     4501 ctcaacccgt ttatgcacga taatcccttt atccatgtat ccacttccgg aggagcggag
     4561 attggttgtc atgtagcttt ggaagtgctg gacgtcttgg aggagcctgg ttttttggag
     4621 catgttcgct cgttagcatt tcaattcaag gagggatttg cccgtctgca agataatcac
     4681 cctgttataa tggttgagcc tcgccaacgt ggtctgatga tgggattgaa gctagctaac
     4741 gccgactatg gacccatcat gacgcaattg ggttttcacg ctggcttgct gactatcttt
     4801 gccaaccatg atccctcagt aatccagatc ctgccgccat taatcatcca gcctggcgaa
     4861 gtcgaacagg ttctgaatat attggatgaa atgttgggca tgttggagat gatggtttaa
     4921 gcccgcggtg cacatacttg aggagttgat ctatcgccca ctctagttta gaaataagac
     4981 gcgcccaaac caagcgaaag gcttttctaa ccaggcactg gacacccttg gtaattatcc
     5041 tggtcgcggt ggttggtgct tctttagctt atctttatcc atcgcctgtt gaatttccga
     5101 tggatgatgc ctatatccac atggtgtatg cacaaaacct ggtggataag ggtagtttga
     5161 tgttcaacga tcctgaagaa aagggtgttg gcacaacaag tgcgtcttgg gtattgctct
     5221 tggcagcggg atatcgcctg ggcatctctc tccatgtggt cttgaaaata ctgggggttg
     5281 catcactgat tggtctgggt gtggggttgt acctcctttt ggttcgatat tgggcagcgg
     5341 tcccggcgct tatcgcgacg ctatttgtca ttctatctgg acatatgctc tggtttgcct
     5401 tgagtggcat ggagacgatc ctttttttga ccattggggt atttgcgtta cttttctatc
     5461 gaaatgagcg ttggatctgg ttgggtatct tccttgggtt actgaccctc acccgccccg
     5521 aggggatact gctagcggtt agtataggac tagtcgatat ttggcgtcgt aaagctctgg
     5581 cgaaagggct tgtgatggct ggtattgtgg ttttcctgat gtgtgcaccc tggtatggtt
     5641 acatcctggc gcgcactgga aatctactgc ctaccagtgg cataggcaaa cgtgtaagca
     5701 gtcaagttgg tatgcacctg ctactgcagc aaaacgaagc gttgtccgta tttgcgaaat
     5761 acccttccct actttaccct tttgtctggt tgggatatct tcttgaatac gtattaggcg
     5821 gggtagcgct gccgccccca gatttgcgcc tggttacctc gattgccagc gaaacttatt
     5881 caatttctgc ctgggccttg cttggctttg taggagtagt cgcacctctg gtttggagag
     5941 gttctcgtca aatatttagc ggacttcgcc agcccaattc gatactgggt gaaaaatctg
     6001 cattaatgtt ggtctgcttg atctgggttg ttctccataa cctgagctac atgtctttcc
     6061 tgccatttcc agggacagcc agccggtatg gggctatgaa tcacatcgtg ttatggattg
     6121 tattgctctc tggcttattt ggtttttggc accggcgagg tatcttcttg tggctggcag
     6181 ctgggttaag cgttatcggc gttgccaata tgggctattg gaatcgcgtg tacgatgcga
     6241 atatcgacca catggtgagc gtacgcattc aggctgcacg atatatcaat agggtgttag
     6301 cggaggaaga gctttgtgca gcaatggatg ttggagcgct gcgcttctac agccagcgac
     6361 ctattgtgga tttaggcggt ttgattgatc ctgagttggg aaagctattt ttgagtgggg
     6421 atttggatcg tcatttagtt gagatgggcg tcacatgctt gattttgcca gggcgagcag
     6481 gggagagcac cgatggctgg tttgatattg ccaaggaact gggcttactc gagagcccct
     6541 ttgttcgttt gcaccaagag aaggtctttg agatggatcg caaacgctgg ctctttggat
     6601 atcaacctac acgcaattac caggccacgg tgacgatcta ccgcatggaa tcgttatgct
     6661 ctatgtgcta gacattaccg gttttgtgat cgcttgcgct gcattttctt cacccaggtc
     6721 gcgtatcgtg agcttagatg aatccagggg gaggacaggc ttgtctcgaa ttgggcttgg
     6781 aacagggcgg ttttgaagct ttctgcatcg tggaaatccg gcatcagcat agtagcccgt
     6841 cccaattcat aggtagtatg tgcgtcggaa ccggccgtac ccagtagcat ctgctggtga
     6901 gcgaattcct gggcttgtgt gttgaaagaa ggcaggaagc aacgcgcgtt aaaggtttcg
     6961 atggcatcca ctagcggggc gatctcgagc agttgggcga gttgccagtg tcccttgcgc
     7021 gttatatcaa atggatgcga gacgctaatg aaagcgcctt gggcgcgcag gtgtgcaatg
     7081 gtctcctggg cggttaaccg tgcagggata tgttcttgga caaaggctgc caggatctct
     7141 ccggattgag tcaggatttc ctcgccaatg atgacccgct ccggatccag ctcctgacat
     7201 tgcattgcac ctgcgatggt gttatggtca gtgaccacca gccggtcaat gcccttgcgc
     7261 ctgcaggctg agagcaatct ctctggtctt gccaggctat cctttgaata cattgtatgg
     7321 ctgtggaact caacgcgaag catggggatt tgggttgggg tcataaatcg aagggatagg
     7381 ccgacgaggt ttaccccctc tccgtggtga ttattctgct ttaagtgatc tgcggccagc
     7441 aaattcgatc aaagccagga tgaggatttc catggtaacg agcagtggac cccagcgctt
     7501 gaatacaata gctgatgcca aggtagctag cagcagaaca aggtacacca tgttgcggat
     7561 tatctgcctg cccgtgcgat atggattgcg gctgctccaa ggctgtgccc aaagtagtac
     7621 aatcacgaac gcccaaaaat tgtactgcca ggccgtctgc gacctgctca gccaggcggt
     7681 gatcaggaac cactccatta gaaccaccac tgccacgccg ctgaggatgt acaacatagg
     7741 cacattctat cacagcagag tttattagat ctatgtattg gtggggaaga atagttgggt
     7801 gtgtgcgggt catgacccaa acacacccaa ttacatctag ccttgaccag ggattatcta
     7861 acagagccat tttttgagtg ttgccctatt cgcggttttt gatatacaat cagtggtatg
     7921 gatcaaaggc tggcgaagca agcctacaag gtgcaaatca tcctggtcag cttgttcgtc
     7981 attctcctat acctatatct ggcgaaccga gcttctcccg atttctcgct gtgggagctg
     8041 atcaaggggg ttgtcaagtt cttcctgctc atgtgggatc gaattcccga aggtgtgatg
     8101 gttttttttc gggatatggt cttggttgtt gctgccttcc tgttttggat tgccttcttc
     8161 gcccaatttg cccttccagt gcgcaatttt ggccagcgta ttctggcgtc aaattacctg
     8221 ttttcctacg ccattggtcg gcacggaccg gctatcaaga ttgataatgg tgatatcccc
     8281 gaccgctacc cgagggataa accacaccgc cggggtgtga tcgtgttgga tactgccagc
     8341 gctgcggtgt tgcgcacgcg cacccaattc acgcgcagtg tgggtcctgg gttggtcttt
     8401 acattgcccg gcgagtatct ggctggctcg gttgacctgc accaaaagat ctcccacgtt
     8461 ccggcgttag gtcctttggc agatgagatg tatcccttcg caccatggga tcaagagaat
     8521 gaggctgagg aagatcatga ggagcgccag aaacgccgtt tcgaaaccag cggggagaca
     8581 cgtgatgcag tggaagttgt accgaatatc catacagtta cgctcatcga tccagaccat
     8641 cggcctaacc agctctcggg tgtcccggca gaccgcgggt tgtacagttg gctgcgtgag
     8701 tacatgcaga gcgattcgca cactcgtttt ggatacaatc ctgaagcggt gcgtttaatc
     8761 gtcactggtg aggcagttga tccgatggtc aatatgccaa tacccgaaaa gcgacaaatc
     8821 ccctggtacc aattcccggc ttatttggcg gtggatctct ggcgcgaata cttgcgtcgc
     8881 tttacctttg aagagctctt ttccaggctg gaagattatg gtggacagac tgctttccag
     8941 gtcatccagg ataagatctt tgagcgcttg acacgccctt atgtgctaga gattaaccaa
     9001 gtgggtaaac caacgggaag actggtcgag agccgtgagt ttgccatgtt gcgagaacgt
     9061 ggaatccggg tgctagctgc tccaattcgt aacctgcgct ttgaaaaaaa gatcgatcaa
     9121 caactggagg accaatgggt gtcgttttgg cgcctgcgcg cggaagccga acgggattac
     9181 attaaccggc ggaggagcta tagcatccac gaaggtgaga aggatgccca gcgcgagttt
     9241 gcattttggg ctactcgccg tattgagagc agcttgctgc aatctccaaa gccaccctcg
     9301 caagcggaag cccgcatcca gatgcgcgtg gtattggagc gtttgttgcg gggtactcta
     9361 ggtcagtgtt tgcatgatac ccggttgcat caacgtttat ccggagaaga gtcgcagttg
     9421 attaccatca tcgaatggtt gcgcaggttg taggctggct aatgaaccga aaccgtaagc
     9481 ccaggactat gaaatacgaa ctaagcctga acgcgatgat cacagactgg cttcgctggc
     9541 tggctcctct taaagatcct gtcttcggcg cccttgatct gctgactgcc tggtttttcg
     9601 acttcggttc tggctgggcg atactccgct ggttcgcaat taccctggta ggcgcagggt
     9661 tatgggtgct tttggcaatc caaccgctgg agaagcttgc tggtgccatt gaccccaatc
     9721 tggtttttga tcaggtgttt tatgtgattt gccaagtgcg ggagacgatt gttgctcttc
     9781 cacctgaaga agctgctgtt catttctgtg gtgcgtacgc aaacccagag gcgatcttat
     9841 tcgagccgct gatatttgaa atcccattgc aatggttgtt cggtgcgggt gtattccgtc
     9901 atatcttgct gctggcgttt gcctgctggc tggcatttaa gatcgccagt cagtatcaag
     9961 cagatgtcta cgatatacca gacatctcct gctccgagca ttttttaatg caggcggcat
    10021 taatcaatcc ccgtaatgtc atcaacatcc agaatgccag ccagcgacca ctggaggagc
    10081 atttgccatt gtacctgatc ggtggtcctg gaaaagtatg tgtgcacctg gaaaatgccg
    10141 cgcttttcga aaagatggat ggggtgccaa gggttgttgg cccaacggtg agacaacctg
    10201 tcaccctgga cggtttcgag cgtttgcgca aagtgattga cttgcgtgat cagaaagaat
    10261 cttttgatat tcgcgggcgg tcgcaggatg gcatccgcgt ggctgccaaa gatgtgcagg
    10321 tagtctacag cgtttttcga aatcatattg aaccttcgta tgaacaaccc tatccttttc
    10381 aagatccaag agctatagaa aacctcatct tccaacaaag ccagcaagta tggaatctga
    10441 gtgtgggcat ccagatccgc ggtgcattgc tggatttctt tgcccaacac accttgaatg
    10501 agttcttggc gatgattcaa gaaccggaat taaagaaagc ccaagaatat gaactggagc
    10561 tcatgaaaga aagccggaga ctatccggtt cagaagagcc aattggcacg cctcccatag
    10621 aaaatgtgga tggtattgaa tacgtttcac gcccgcaaat caccgacctg ttctatgagc
    10681 gggctgagga gaccaagctg gcgcgcggtg ctgaaatcga ttgggttgga gttggaactt
    10741 gggattttcc agcccagctc atccccgtgc gccatcagga agcttggcgc attacccatg
    10801 acaacctgat gaaaaataat ccagcggtat ttaatggttt gcgttggcag tataaaatca
    10861 acgagaccct gcggctgatt cagatggtgc ccattgcaac tttcaaaaaa atgatcagca
    10921 cccccgatat ccacaccgat gacgcaattc gtgctctgat tttagcttat catacccaga
    10981 tgcgagaagc atatgccgat tatgagcgtt cggaacgtga gctcgaaaag cttgaaagcc
    11041 aggagactga cccggtgaaa gtgctggagc accgcgagaa actcgggaaa ttgatcgcag
    11101 aaaagcagag gatttccgag gtaatggctt tccttgcacg ctttattggt cgctggctgg
    11161 taggaccgtc tctaactgga ccaacaccga aaggtccatt accgactggt ccccaggcgg
    11221 ctgagtctgg caacggaggc taaggcgatg tttgatagca tttccccaaa tccagatggt
    11281 ccagtactgg tgattggcgc ggcaggagtg gatattgttg gcaggctgcg gggagagctg
    11341 cggtcaaaga cttcgacacc ggcggtgatt cgctcctcct atggcggggt agcccgcaat
    11401 gttgcagaga atctggttcg cttagggcaa cctgtaaacc tgattagcgt ggtaggagag
    11461 gatgatgagg gggatcgcct gttgcaaggc ttgattgctg ccggagtgaa tccggagcga
    11521 gttttgcgca cttcccaata tgtgacaaac tcttatctgg cgataataga tagtcaaggg
    11581 gagttgcaat ttgcgctcga tgatatgcgc gctatcaaca gcctgacgcc ccagtttatc
    11641 cgtgagcagg ctgcgctgtt caaagaagcc tccattctct ttgtcgatgc caatttgccc
    11701 aaggatactc tccgcaccgt gatgtcgctg gcgcgccggg cgcatttgcc ggtatgcgcc
    11761 gatcctacct cggtttcact ggcggcccgc ttgcgccctc atttatcacg attctttttg
    11821 acgatcccaa ataacgctga agccgcagta ttatccgagc gtacatcgcc agtcaatgga
    11881 cgccgccagg cgttagaggt ggctaagtat ctcgtatcac agggtgtaga tattgttatc
    11941 atcaccatgg cagagcaagg tttgtgttat gcgacttcgg agagttctgg ttatatccca
    12001 gccatcaaga catcgatttt agatccaacg ggtgctggtg atgcactcac ggcgactgtg
    12061 atttttgca
//
LOCUS       DBNGSX010000057        21070 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_3563, whole genome shotgun sequence.
ACCESSION   DBNGSX010000057 DBNGSX010000000
VERSION     DBNGSX010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 21070)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21070)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21070
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_3563"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="AC16CB_01825"
     CDS             complement(<1..326)
                     /locus_tag="AC16CB_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3341233.1"
                     /translation="MVDKKHVHGKNSLVWMEKISKKFGTITALQDVDFGVEGGEVRAL
                     LGDNGAGKSTLIKVLMGVYPPTTGQIYFQGQPVRISSPREAREVGIEAVYQDLALVNL
                     MSISRN"
     gene            complement(358..1362)
                     /locus_tag="AC16CB_01830"
     CDS             complement(358..1362)
                     /locus_tag="AC16CB_01830"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3341234.1"
                     /translation="MIVEDTWRKRLAHSFSMSTVLGPLVVLIIIFLLFSIFVPNFLTF
                     RSITGIINAITLTGTLTVGVAMLMISGEFDLSVGSIMAVGAYIYGGLTMAHQPALGFI
                     LALVVPALLGMVNGLILVWTGIPSFIVTLGTKYLFRGLLWVVTAGTMLQTIDRLPIYN
                     LFNGRLDIVNNLFTGANFRSSLLWLLMLVIFFQFILVRTRFGNHVFAVGGNQGAALGQ
                     GVNVKFVKLMGFIITGALAGFTGVLLFSQYYTVRVASGDGLELSAIAAAVVGGTLITG
                     GAGSIWGALVGALIISMLRTGVVLMNLPYIPADNFEAIIGVTIIAAAILNKYLRGQSS
                     "
     gene            complement(1448..3415)
                     /locus_tag="AC16CB_01835"
     CDS             complement(1448..3415)
                     /locus_tag="AC16CB_01835"
                     /inference="COORDINATES: protein motif:HMM:NF024799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR3341235.1"
                     /translation="MKKLNILLVLLVVFTLLLTACQPQPTPAPAEPTQPPAAEQPPAE
                     QPTEAPPAEQPTQPPAPEATEPPAPPPAEGALNLIMVQHALCAWDSFWCTVEDGIKTA
                     AAQMGVEVTILGPDKFDLEKTASLIDQAVAAQPDGIGLTVTDPVLFKEPIMKALDAGI
                     PVLAYNAGKGPLEDGIPYMTYLGMDEYQGGYQSGQRMAAAGASSGVCINHQVGHAGLD
                     ARCKGFVDAFTEKDLPAEVLAIKGEDAAQSQTTISDFYAANPDVDAFLTLGPSGANPF
                     YAFLEAEQPDGVMHGTFDLSPEIEANIQDGTTMFAVDQQPFLQGYNAVVYLVLANKYG
                     IRPALPVTATGPGFIDKNLVGKATQPDRPVKLIMVQHALCAYDPYWCVVEIGMQEAAE
                     HMGAELTILGPDKFDVEKTAALIDQAVASAPDGMGVVITDNQMFKPPLMKAVDAGIPL
                     VAYDTGLGPEADGIPYMTFWGAEDYVQGYYSGVRMGDEGATKGVCINHQVGQTGLDAR
                     CRGFVDALAEKGIPAEVLGVKGEDAAQVQTTISDYYTANPDVNAFLNLGPAGSDPFYA
                     FVEAEGIQPGQIIHGTFDISPAGVKAIKDGVTLFILDAQPYLIGYGTVESLVNYLRYN
                     ITPATGISATGPGFIDKSNVDIVEALAGTYR"
     gene            complement(3561..4559)
                     /locus_tag="AC16CB_01840"
     CDS             complement(3561..4559)
                     /locus_tag="AC16CB_01840"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3341236.1"
                     /translation="MSAFADKIAVMRYRLSYIPEAVALISFIALFLFFGFAAPNFLTP
                     LSIGNILTFGSITAIVTIGVAILMVAGEFDLSVGSNFAVTSYVIALSLIAGLSVWIAI
                     PLGILVSTLLGMLNGLIVTKTRIPSFICTLGTMLAYRGIVRAIGKGSFATYKGEPIPL
                     FSVLNGDFDLLNNVFGMQAFRTSMVWFILLAIIFSFVLMRTRFGNWIFAGGGAADAAR
                     AQGVDVTRVKILCFTLTGFLVALASTLQFAHRLSVDPLRGDGMELIAVASCVIGGVRL
                     TGGYGTVFGAILGTFLLQMLDQGLVLMQIPIQVFQACVGLIIIISVILNTYLGKSD"
     gene            complement(4721..5923)
                     /locus_tag="AC16CB_01845"
     CDS             complement(4721..5923)
                     /locus_tag="AC16CB_01845"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011605756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR3341237.1"
                     /translation="MPGPGYYFFGEEERKEVEDVLTSGYLSRYGREDDPLFKQKVFTF
                     ESEFARKMGARYCVAVNSGTSALMAALVAIGLQPGDEVLVPGYTFIASMSSVIAVGGH
                     PVLVEVDESLTMDPVDVERKITERTRVIMPVHMLGNPCDMERILTLAEKYNLIVIEDC
                     CQALGGSYCGQRLGTYGAFGGFSLNNYKVINSGDGGVLITNNQELYKRAFAFHDQGHL
                     PLRKGVEIGQRTLMGVNMRMNELTGAFALGQLRKLDLILSTLRKKKALFKQNIQVGDI
                     RNMQFRRINDVEECATLLTVQFPDEALTEKVVKALQTKSVYNSGWHVYNHMEQFLSYT
                     DASGKHPYHKNMLPQTDEILRRSVNLSVGVVDPGLGADFGINVLSSDDEIQQKAEEFI
                     RIVKPIAG"
     gene            complement(5988..6824)
                     /locus_tag="AC16CB_01850"
     CDS             complement(5988..6824)
                     /locus_tag="AC16CB_01850"
                     /inference="COORDINATES: protein motif:HMM:NF016668.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HIR3341238.1"
                     /translation="MKASEVLGYPKDARLLIVNIDDYGLCFTVNDTAIQIINKAAASS
                     CTVMTPAPWGIHGIDLVKANPGVKCGVHLTAISEHVCYRWKPLSSPETVPSLIDSDGY
                     FFLETYQDQFTRQADVHQLEREWRAQIEFALRRGLQVSHLDSHCNVHDSRDDFFAMTY
                     SLAQEYGFPLRIHNEKYLHQAKKEGRVTIDYPDLDSFRVPLENKSLLYVQMLRELPEG
                     LSEWAIHPAYDSAELRAITPEWPVRVTDYEFFSSDEFLHVIQTEGIELIDYSMLKPYW
                     VP"
     gene            complement(7072..8517)
                     /locus_tag="AC16CB_01855"
     CDS             complement(7072..8517)
                     /locus_tag="AC16CB_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HIR3341239.1"
                     /translation="MNIQLSNGNVIPVEMHKVRIIQKTSLPPIDRRLAAIQEAGYNTF
                     LLRTRDIFLDMLTDSGTNAMSDNQLAAMMVSDDAYAGCESFYKLADAVKDVLGFNYVI
                     PVHQGRAAEHLLAKAFVKPGDVVLMNYHFTTTKAHFELVGASVLELYTAEALNTQSSE
                     PFRGNLDLNRLKDAIQEHGADRVSFIRMEATTNLIGGQPFSLCNLRAVKEIAEANNIP
                     LVFDGSLISENAYFIKMRECEYAAQSITKLIQEMMKMVDIFYMSGRKSTSVRGGMMAT
                     NHKEYYDRILAWLPVYEGFATYGGMSTKEIEAMAVGLREMTDVNVAGSSPEFIQYFVN
                     RLVENGVPAVTPAGGLAGHVDASRFLHHIPQSQYPAGALAAAIYLASGVRSMERGTIS
                     MDRDLDGNEIYADLELARLALPRRVYTLSHIEYVVDRLTWLYEHRHLVKGLRFVEEPK
                     VLRFFFGKLEPLDHWGYQLAEAFKAEFGENF"
     gene            complement(8880..9956)
                     /locus_tag="AC16CB_01860"
     CDS             complement(8880..9956)
                     /locus_tag="AC16CB_01860"
                     /inference="COORDINATES: protein motif:HMM:NF048802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7544 domain-containing protein"
                     /protein_id="HIR3341240.1"
                     /translation="MDHMQTLKRAWNILWSYRALWVIGIILALTSPSGFSPGSSNFSP
                     DLNVPGRYETTPPEEIQRELEKLGELFNQGIPADTLETLTRIGVLFVLAMLLLAILFA
                     IGYYVSQAALIRMVDKYEQDEEKLTWKAGLRLGWSRAAWRLFLIDLVIYLPIFLAFIL
                     IFGCAALPVLLKTLAGQQPTMPGIIASVGLVFLLIFVAIIINVILSLVLEIIRRVGVL
                     QGSDAITSIRQGLEVIRGNFKDVFIMWLILLGIKVGFFIALIPVAILLAALGLFVGGG
                     VGFLFYTVFQATTSTLVAWIAAVLSGGILFILVLAIPMLFLEGLRETYLSSTWTLTYR
                     ELNLEMLDRSSSPEESDPSADVVA"
     gene            complement(9976..10551)
                     /locus_tag="AC16CB_01865"
     CDS             complement(9976..10551)
                     /locus_tag="AC16CB_01865"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR3341241.1"
                     /translation="MNGHLEIWIEAYLDDELSSLHRHQVEAHLGECQRCRALLEERKN
                     LSALLMALPDPANLKSEARFVAEVGLQLKQRRKALQIIPMVPDLGWYFIPVVLLLVLT
                     FFQTVAILSNMLWVVPGAKLILLDQVSFVSVLPSLPEPLSALINMMDLLSPLNGGFFT
                     GIGAGLLISLMYVCWLVGWWVWKQRTAVSQV"
     gene            complement(10548..11111)
                     /locus_tag="AC16CB_01870"
     CDS             complement(10548..11111)
                     /locus_tag="AC16CB_01870"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3341242.1"
                     /translation="MMMLEAVSVGDESKLISLAQEGDRQAFAELVSLYRLRVINMVYR
                     MCGDARVAEDAAQEAFIRVWQHLPDLRPGSSFRNWLYRIAVNSALDITRREKPNVDID
                     SLDLASPGEHLEVRLERRESAREIRQAVLSLPHASRVVLILREYEGLSYREIAATLDI
                     SIGTVMSRLHYARTRLVEILRPLMEAT"
     gene            complement(11447..11860)
                     /locus_tag="AC16CB_01875"
     CDS             complement(11447..11860)
                     /locus_tag="AC16CB_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3341243.1"
                     /translation="MDWNSRLEISQSEVQGRVPITILHVAGRINMSSTDILENAVRVA
                     YEKGARYFLLDLNKVPSLSSVGLRSIHWIYNILQSASQVGGEAKSGTAVGGTPVSPYV
                     KLLCSSPQVLKVLNMAGFDSYIEVYGELQEAISAF"
     gene            12401..12859
                     /locus_tag="AC16CB_01880"
     CDS             12401..12859
                     /locus_tag="AC16CB_01880"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3341244.1"
                     /translation="MNVGKSRQYTAGLNDLAEIRHFLQKRAAELQVDPSTTYDLLLAV
                     TELVTNTMIYGYQNEPGFIEAEMYLEKDSLIIHLRDHAAPFNPTQFPPPDLSISLEKR
                     AIGGMGIFLAKQLVDQMIYRELPQGGNEVTLVIHQISTRQKEEGNDEDRN"
     gene            12843..13253
                     /locus_tag="AC16CB_01885"
     CDS             12843..13253
                     /locus_tag="AC16CB_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3341245.1"
                     /translation="MKIEIEFTKGKIPVTILSLQGDLDASNFESVIARARDLYTEGTR
                     HLLLDLSELRFMSSSGLVAMHSIVLLMRGTDPHDLLEGWNAIHAIDRDRASEPQSYVK
                     ILNPQPKVANTLKMTGLDQFFEIYQDRQKAIASF"
     gene            13256..14494
                     /locus_tag="AC16CB_01890"
     CDS             13256..14494
                     /locus_tag="AC16CB_01890"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR3341246.1"
                     /translation="MDTSLIRQVPLFSSLEPSGIELLAAALKECHYPAKTVLFKEGDY
                     GDKFYIVAEGEVAIIKAIGTPNERLLAIRGSGQFIGEMSLLNRDGLRTASARVHSDAR
                     LLELSREDFNALLDREPPLAYQMLSVLSQRLQTSQNQTIRDLSEKNRQLAEAYENLKA
                     AQEQIIEKKILERELMQAREIQESMLPTVLPRLKGFDIVAQMLPARMVGGDFFDVIRL
                     SPKSLGIVIGDVAGKGVPAALFMALTRSLLRAEAKPLVSPEIVLQNVNHTLLDMNAKG
                     LFVTVLYGILNQETREFNYVRAGHESPMLWDKHGKIIPTVLGKGQVLGLLDHPILEPR
                     SLTIPLGGAMLLFSDGVTESANQQEEFFGTQGVEAIIPDLLNKPVQDQLDHLLQEVSH
                     HQGTNPQADDITIIAVQAHA"
     gene            14511..15479
                     /locus_tag="AC16CB_01895"
     CDS             14511..15479
                     /locus_tag="AC16CB_01895"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HIR3341247.1"
                     /translation="MITVQDVQDARRRIEPYVRRTPLERNDTLSKKLGSNIYLKFELF
                     QKTGSFKPRGAFNQILQLSQQERDKGAVAISGGNFAQGVAYAGQVLGMRTIICMPDYT
                     PRNYIQATQAYGAEVLLSPTFAETIELAQSYQRRGFSFLHAWDNPNQMAGNGTLGLEL
                     LEDLPELTDLIISIGGGGLIAGIIVAIKALKPGVRIWGVETEGADTMGQALKAGKVVQ
                     IQPTSLAKTLSAPYVAEDALLLTQKHLEKYILVTDREAFDAQLFLLERAKVITELAAS
                     CTLAAAEQISGAFEKNNHVILLLCGGNASLDNLMEYHRQLRGEPEP"
     gene            complement(15532..18942)
                     /locus_tag="AC16CB_01900"
     CDS             complement(15532..18942)
                     /locus_tag="AC16CB_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3341248.1"
                     /translation="MSPIAERYPSTSSSLPTGTLTYLFTDVEGSTRLWEQYPDQMRGV
                     MIRHDDLIESLVTNYGGSVVRPRGEGDSRFAVFPRAADAVAAASAIQRALSFEAWIIP
                     SPLRVRMGLHTGAADLRDNDYYGPAVNRCARLRNAARGGQVLVSQNTNVLVRDALPEG
                     VSMRDLGEYRLKDLNRTEHIYQLVIEGLPGDFPPLKALENLHTNLPAALTSFVGRERE
                     IEEVKQLLTQTRLLTIAGVGGAGKTRVALQVAEDLLEFFGDGVWWMDLAPISNPELII
                     QFVADVLGIREEAGKSLLQLLTSYMRTKNTLLVLDNCEHLIQGVALFVETILRSAPEL
                     RILATSREALGMSGEVIWRIPPLSAPGLRERVKSENLVQYEAVKLFVERAVAVKADFE
                     ITVENARSVAKICARLDGIPLAIELAAARVRVLSVEEIAERLDDRFRLLVSGNRTALP
                     RQQTLRALLDWSHELLSEHEQILLRRLSIFTGGWTLPAAEKVCFDEEVEAWQILELLT
                     HLIDKSLVVAESHDRSERYRYLETIRQYAQERLLEAKEVDELACKHAEYFLNLAEEAY
                     SELWGPRQATRLVQLEAEHENLRMALEYMSKDGSRAEMLLRLGSSLWRFWEIRGYISE
                     GRAWLKRALDANPDAPAYLRGNGLRGAGILACQQGDYMQAKALHEQSLTLFRELGNKL
                     GIARELNDLGEIARYLGNYQEAVALHTESLALRYEIDDKEGLAVSLGQLGTIALDRGQ
                     HIHARELLEEGLKLNRELGDKQSTALSLNNLGLVSQLLSDFVRAISLFEEALSLYREL
                     KDKLGISNALLNLGSVAKDQGYFKQATTLLNECLVLKQEVGDRRGIGRIIAALAEIAV
                     HQGNYARGAELAEQSLKIFRELGVKRGMIISLEVRVYAANHQGDYDLAQSLASQALTL
                     ANEIGAPRGIAYSKAFLGLIAYAKGNLTEALKLLHSSLDIFRDVGDRKDVAYVLVNLA
                     RVAYRQGDNSAASQMLEESLTISRELDIRWGLAFSLEIMGLLQRSEGHNDQAFSLFKE
                     SLILSAEQENLQGILNCLGALAGLAAMTGQPSLAARLFAVTDKMRDSTGARMGSDDRM
                     EYERYLSVLRNQMSDAEFESTWSGGYAMTTEQAIAELEGWSINPTSHS"
     gene            complement(18926..19342)
                     /locus_tag="AC16CB_01905"
     CDS             complement(18926..19342)
                     /locus_tag="AC16CB_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3341249.1"
                     /translation="MMKTGELGKVYQDRELIIRQGEAGDCMFVIQQGQVEILSEKDGK
                     EIRLAVRGEGEFFGEMAIFEREVRMATVRALGEVRVLTVDKKNILQRIHEDPSLAYRM
                     IQTMSRRIREQSQEVVRLTNLIKEKGALLDNDVPHR"
     gene            complement(19407..20774)
                     /locus_tag="AC16CB_01910"
     CDS             complement(19407..20774)
                     /locus_tag="AC16CB_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341250.1"
                     /translation="MDGSNNLKLKDGSRVAVIGGGPAGSFFSYFLLQFAERMGLEIFV
                     DIYEPRDYSKPGPTGCNMCAGVVSESLVQNLATEGIILPSTVVQRGIDSYVLHMDVGS
                     ALIPSIRDERRIGTAFRGVGPRGIKEIKWDSLDHFLMKLALDKGAQHIPARVSEVEKV
                     ADPEKLNGGGQRLQVKTTKGLTQTYDLLAVSAGVNTAILKLFQGMDFGYQPPLTTQCF
                     VREYYLGEEAVSKHLGSSFHAFLLNIPNLDFAAMVPKGDYASLALLGENIDADLLQTF
                     TSDPALERCMPPNYPMDQVDCWCSPRINIKGSARPYGDRIVFIGDSAVSRLYKDGIGA
                     AYRAAKAAATAAIFEGVSSEAFERRYWPACRSMEIDNTIGKILFAVVREVQKRKFARR
                     AILQMVLSEQKRGVVGDRNMSMVMWDMLTGSAPYRKILLRTLHPVFWMRLLKHFFVAA
                     VQSFG"
BASE COUNT         5464 a         5737 c         5030 g         4839 t
ORIGIN      
        1 aaattgcgcg agatgctcat caggttaacc aatgccaggt cctgatatac cgcttcaatg
       61 ccgacctcgc gtgcctcacg cggcgaggag atcctcacgg gctggccttg aaaatagatc
      121 tgacctgtgg tgggagggta tacacccatc aagactttga tcagggtgga tttcccagct
      181 ccgttatcgc ccagcaaggc gcgaacttcg ccgccctcca cgccaaagtc tacatcttgc
      241 aacgcagtaa tggtcccaaa tttcttggat attttttcca tccagaccag ggaattctta
      301 ccgtgcacat gttttttatc caccattttt acactccgaa gagatgatcc agggtcgcta
      361 agatgattga ccccgcaggt atttatttag gattgccgcg gcgatgatcg tgacaccgat
      421 aatggcttcg aagttatcgg cggggatgta cgggaggttc atcaacacca caccagtgcg
      481 caacatgctg ataatcaacg cgcccaccag cgcgccccat atactcccag ctccacctgt
      541 gataagcgtg ccccccacga ctgcggcggc gatagcactc agctctagcc cgtctccaga
      601 agccactcgc actgtgtagt actggctgaa aagaaggact cccgtgaagc ccgccagagc
      661 gccggtgatg ataaagccca tcaatttgac aaatttgaca ttaaccccct gacccagagc
      721 agcgccctga ttcccgccaa ccgcaaagac atgattaccg aagcgggtac gcaccaggat
      781 aaattggaag aatatcacca gcatcaacaa ccacagcaga gaagatctaa aattcgcacc
      841 ggtaaacagg ttgtttacaa tatctaaccg accattgaat aagttataga ttggcagccg
      901 atcaatggtc tgcagcatgg tcccggcagt gaccacccac agcaacccgc ggaacagata
      961 ttttgtcccc agcgttacga taaaagaggg gatacctgtc caaacaagga ttaacccgtt
     1021 caccatgccc aagagcgcag ggacaaccaa cgccaggata aatccaagag caggctggtg
     1081 tgccatggta agcccgccgt agatataggc tcccactgcc atgatcgagc caaccgaaag
     1141 gtcaaactcg cccgagatca tcaacatcgc aacccccacg gtgagtgttc cagtgagcgt
     1201 aatggcgttg ataataccgg taatcgagcg gaaggttagg aaattgggga cgaaaataga
     1261 aaaaagcaaa aagatgatga ttaagaccac gagagggcct aggacggtcg acatagagaa
     1321 tgaatgagcg agtctttttc tccaggtatc ttcgacaatc attttcttct cgccctccgc
     1381 ataccgacag agcttgggtg agcgggcagg actttcagcc ctgcccgctc tgaggtttta
     1441 acgatattta cctgtaggtg ccagccagtg cttcgacaat atcgacattg cttttgtcga
     1501 taaagccggg gccagtggca ctgatgcccg tggcaggcgt gatgttatag cgcaggtagt
     1561 tcaccagaga ttcgacagtc ccatagccga tcaggtacgg ctgggcatcc aggatgaaca
     1621 gggtaacacc atccttgatc gccttgacac cggcagggct gatatcgaag gtgccgtgga
     1681 tgatctgacc gggctggatg ccttcagctt cgacaaaggc atagaacggg tcggaaccgg
     1741 caggtcccag attcaagaag gcgttcacat ccgggttggc ggtatagtag tcgctgatgg
     1801 tggtttgaac ttgggctgca tcttcgcctt tcacacctag cacttcggcc ggtatgcctt
     1861 tctcagccag cgcatcgacg aagcctctgc aacgagcgtc aagaccagtt tgaccaacct
     1921 ggtggttaat gcagacgcct tttgtggcac cctcatcacc catgcggaca ccactataat
     1981 aaccctggac gtagtcctct gcgccccaga aggtcatata ggggatccca tccgcttccg
     2041 gacccaggcc cgtgtcgtaa gcgaccagcg ggataccagc atccacagct ttcatgaggg
     2101 gcggtttgaa catctggttg tccgtgatca ccacccccat gccatctgga gccgaggcca
     2161 cagcctggtc gatgagcgcg gcggtcttct caacatcaaa tttatcgggg ccgaggatag
     2221 tgagctctgc gcccatatgc tcggcagcct cttgcatgcc tatctcaact acgcaccagt
     2281 atgggtcgta agcgcatagg gcgtgctgaa ccatgatgag cttgacgggt cgatccggct
     2341 gggtcgcctt gccaaccagg ttcttgtcga tgaaacccgg accggtggcg gtgacaggca
     2401 gcgccggtcg gatgccgtac ttattagcca gcaccagata gacaaccgcg ttgtagcctt
     2461 gcaggaaagg ttgctggtcg acagcgaaca tggttgtgcc atcttggatg ttggcttcga
     2521 tctcggggct gagatcaaag gtgccatgca tgacgccatc gggttgttca gcttccagga
     2581 aagcgtagaa gggattggcg ccgctaggcc ctagcgttag gaacgcatcc acatccgggt
     2641 tggcagcgta gaaatcggag atggtggtct gtgactgggc agcgtcttca cctttgatgg
     2701 cgaggacctc ggcaggaaga tccttctcag tgaaggcgtc cacgaagccc ttgcagcggg
     2761 catccaaacc ggcatgaccg acctgatggt tgatgcaaac tccgctggaa gcgccagccg
     2821 cggccatgcg ctgaccgctc tggtatccgc cctggtactc atccatgccc agatacgtca
     2881 tataggggat gccgtcttct agcgggcctt tgccagcgtt atatgccaaa accgggatgc
     2941 cagcatcgag ggccttcata atcggttcct tgaacaacac cgggtcggtg acggtcaggc
     3001 cgatgccatc cggttgggca gccacagcct ggtcgatcaa ggaagcggtc ttctccagat
     3061 cgaacttatc tgggccgagg atggtgactt cgacacccat ctgagcggca gccgtcttga
     3121 tgccatcttc aaccgtgcac cagaaggagt cccatgcgca cagggcgtgc tgcaccatga
     3181 tgaggttgag ggctccttcg gctggtggtg gagcaggcgg ctcggtcgcc tccggtgcag
     3241 gcggctgcgt gggctgctcc gcaggtggcg cctcggtagg ctgctcagca ggcggctgct
     3301 cggcagcggg tggctgggtt ggttctgcgg gcgctggcgt gggttgtggc tggcaggctg
     3361 taagcaacaa tgtaaacacc acaagtaaaa ccagcaaaat atttagcttt ttcattcttc
     3421 ctccatattt acaaatcttg agagagttgt cttacactga gaataaacag cttggaatat
     3481 ttttcacata tggcgttatc aggtcatcac ctccttatga tccaaagaaa gagttttgca
     3541 tccaatagtg aagagaggct ttaatcgctc tttcccaaat aggtgttgag aattactgag
     3601 atgatgatga tcaggccaac acaagcctga aacacctgta tagggatttg catcaggaca
     3661 aggccctgat ctaacatctg gagtaagaaa gtacccaaga ttgcgccaaa cacggttccg
     3721 tatccaccgg tcaggcgcac gcctccaatc acgcaggatg caaccgcaat aagctccatc
     3781 ccatcaccgc gcagcgggtc tacactcaaa cggtgcgcaa actgcaaggt gctggcgagg
     3841 gcaaccaaga aacccgtcag cgtaaaacac aggattttca ccctggtcac atccacccct
     3901 tgcgcccgag cagcatctgc cgctccaccc ccggcaaaaa tccaatttcc aaaccgcgta
     3961 cgcatcaaaa caaacgaaaa aatgatggct agcagaataa accagaccat ggaagtgcga
     4021 aaagcctgca tgccgaagac attattgagc aggtcgaaat cgccatttaa gacactgaat
     4081 aacgggatcg gctcgccttt gtaggtggca aaactgccct ttccgatggc acgcacgatc
     4141 ccccgatatg ccagcatagt gcctaaagtg cagatgaatg atgggattct cgtcttggtg
     4201 acaatcaatc catttaacat cccaagcaag gtgctcacaa gaatcccaag gggaatggcg
     4261 atccaaacag atagaccagc aatcaaactc agggcgatga catagctggt gaccgcgaaa
     4321 ttcgagccca cggagagatc aaactcacct gcgaccatca atatcgccac gccaatggtg
     4381 actatagctg tgatgctacc aaaggtcagg atattcccaa tggataaagg ggttagaaaa
     4441 ttgggggcgg cgaaaccgaa gaatagaaac agggcaataa aactgatcag cgcgacagct
     4501 tcggggatat aagataaccg gtatctcatc actgcaattt tgtcagcgaa cgcgctcatt
     4561 cttcacctcg aagagtgctc tgcattcttt cattacatat cccctctgta ttgccaaatc
     4621 cactttatgc aaatgtttgc aaagagtcta gcatacatta ttttgcttgt caattctcgc
     4681 atcaccggat aggtgagaat cataaatatt aacgctgatc ctagcctgca attggcttaa
     4741 cgatgcggat gaattcttcg gctttttgtt gaatttcatc atcgctgctc aaaacgttga
     4801 tcccaaagtc tgcgcctagg ccagggtcca ccacgcctac gctgagattt acgcttctac
     4861 gaaggatctc atcggtctgc ggcagcatat tcttgtggta ggggtgtttg ccgctcgcgt
     4921 cggtgtaaga caggaattgc tccatgtgat tgtagacatg ccaaccgcta ttgtagacag
     4981 atttggtttg taaagcttta accacctttt cggtcaaggc ttcatcgggg aattggacag
     5041 tcaacagggt ggcgcattct tctacatcgt taatgcgacg gaactgcata ttgcgaatat
     5101 cgcctacctg tatgttctgt ttgaacaaag ccttcttttt tcgcaaggtg gagagaatca
     5161 agtccaactt tcttaactgt cccaaagcaa aggcgccagt taactcgttc atgcgcatat
     5221 tgacccccat cagggtgcgt tgaccgatct ccacgccttt gcgcagggga agatgtccct
     5281 gatcatggaa agcaaaagcg cgcttgtaaa gctcctggtt attggtgatc agcacacccc
     5341 catcaccaga gttgatgacc ttataattat tcaacgagaa gcccccaaat gccccatacg
     5401 tgcccaaccg ctgcccgcag tatgagccac ccagcgcctg gcagcaatcc tcaatgacga
     5461 tcaagttgta tttttctgcc agggtcagga tgcgctccat atcgcagggg ttgcccaaca
     5521 tatgcactgg catgatcaca cgggtgcgct cggtgatctt tcgctcaacg tctactggat
     5581 ccatggtcag ggattcatcc acctctacca gtacagggtg cccgccgaca gcgataacag
     5641 acgacatcga ggcgataaag gtataaccag gcaccagcac ttcatctccg ggttgaagtc
     5701 cgattgccac cagggcggcc atcagcgcgc tggtacctga atttaccgcc acgcaatagc
     5761 gagctcccat ctttcgggca aactccgatt caaacgtaaa taccttttgc ttgaacagcg
     5821 gatcatcttc cctgccataa cgcgacaggt aaccgctggt gagtacgtct tctacttctt
     5881 tgcgttcctc ttctccaaaa aagtaataac ctggacctgg catgtatgcc tcctgagaat
     5941 agcagttaga gggttccgga aaattccggg ctttcgaagc ccgactttta aggcacccag
     6001 taaggtttta gcatggaata atcaataagc tcgatccctt cggtttggat gacatgtaga
     6061 aattcatcgg agctgaagaa ttcataatcg gttacccgta ccggccattc gggggtgata
     6121 gcgcgcagtt cggcgctgtc ataggccggg tggattgccc attcagacaa tccctccggg
     6181 agttctctca gcatttggac atagagcagg gatttgttct ccaagggcac cctgaagcta
     6241 tccagatcgg gataatcaat cgtcaccctg ccttctttct tcgcttgatg taaatatttc
     6301 tcattatgaa ttcgcagagg aaatccatat tcctgcgcca gggagtaagt catcgcaaag
     6361 aaatcgtctc tcgaatcgtg gacattgcag tggctgtcca ggtgagaaac ctgaaggccg
     6421 cggcgcagcg caaattcgat ctgagcccgc cattcgcgtt ccaattggtg gacatcggct
     6481 tgacgggtga attgatcctg gtacgtctca aggaagaaat aaccgtcgga gtctatcaac
     6541 gaggggacgg tctctggaga agacaacggt ttccaacggt agcatacgtg ctcgctgatg
     6601 gcagttagat ggacgccaca tttcacccca ggatttgctt tgaccagatc aattccatga
     6661 atcccccacg gggcaggtgt catgaccgtg caggaactag ctgccgcctt gttgattatc
     6721 tggatagcag tgtcattgac cgtaaagcat aagccataat cgtcaatatt gacgatcagc
     6781 agacgagcat cttttggata accaaggacc tcacttgcct tcacgggaat tcccatggat
     6841 aacttgagat attctggatc aatactggat aaggagactg ccctttactt tatgcacacg
     6901 tgtgcataag ctattttact acacttttga attcggaact tatgactgca aaattaatct
     6961 ctatcaatga tatggctctc caacagcaca atgttggaga gccatatttt cctaaacgat
     7021 ggattattcg cgattaccag caaacaacct tgtctttatt cacgtctggt tttagaaatt
     7081 ctccccaaac tcagccttga atgcctctgc cagttgatat ccccaatgat ccaatggctc
     7141 cagtttgcca aagaagaaac gcaacacctt gggttcctcc acgaagcgaa gccccttgac
     7201 cagatgacgg tgctcatata accaggtcag tcggtccact acatattcga tatgggagag
     7261 cgtgtacacc cgtcttggca gtgccagccg tgccaattcc aagtctgcgt agatctcgtt
     7321 accgtccaga tcacggtcca tagagattgt gccgcgctcc atgctgcgca cccccgaagc
     7381 gagataaatg gctgccgcca gtgccccggc cgggtattgc gactgcggga tgtggtgtaa
     7441 aaagcgactt gcatccacat gtcccgccaa tcccccagcc ggcgtcactg cagggacgcc
     7501 gttctcaacc aatctattga caaaatactg gatgaattcg ggcgagctac ccgccacatt
     7561 aacatctgtc atctcacgca ggccgactgc catggcttcg atctccttgg tggacatccc
     7621 accataggta gcaaatccct cgtaaaccgg cagccaggcc aagatgcggt cgtagtattc
     7681 tttatggttg gtggccatca taccgccgcg caccgaggtg cttttgcgtc cagacatata
     7741 gaagatgtcc accattttca tcatctcttg gatcagcttt gttatggatt gagcggcgta
     7801 ctcgcattcg cgcatcttga tgaaataggc gttctccgag atcagactgc catcgaacac
     7861 cagggggata ttatttgcct cggcaatttc cttgaccgct cgcaggttgc acaacgagaa
     7921 aggctgcccg ccgatcagat tggtcgttgc ctccatgcgg ataaaagaca ccctatccgc
     7981 gccatgctcc tggatagcat ccttcagcct gttcaaatcc aggttacccc tgaacggctc
     8041 gctgctctgg gtgttcagcg cttcggccgt atatagttcg agcacacttg ccccaaccag
     8101 ctcaaaatgc gccttggtcg tggtaaagtg ataattcatc agcaccacgt cacccggctt
     8161 gacaaaggct ttcgccaaca ggtgttccgc tgctcgcccc tggtgaactg ggattacata
     8221 gttgaagccc aagacatctt ttacggcatc tgccagctta tagaagcttt cgcaaccagc
     8281 ataggcgtca tccgacacca tcatggcagc tagctggtta tcgctcattg cattcgtacc
     8341 gctatcggta agcatatcca gaaagatgtc cctcgtgcgt aacaagaagg tgttataacc
     8401 cgcctcttgt attgccgcaa gccgacggtc aatgggcggc aaactcgtct tttgaatgat
     8461 acgcactttg tgcatctcca ctgggattac gttaccattc gaaagttgga tattcatttg
     8521 tcttatctcc ttctgtgggt atcacacaga atacgcttta gcgccaggtg atgaattcaa
     8581 aaccaagttt catccctagt tcgtatggta ttaaacaaag cgcaacaggc aaattaaaat
     8641 cggattttcc cctcctcttt taagacacca tacccccaca cacttcctaa gaatttgtcc
     8701 ctctacggga taagccaaga tcaaacttgt gatgagcata caattacttg ctcacattta
     8761 tgtcacattt aatttacaga atagcataat tgtcaatcaa aatcaagcaa aaaagggttg
     8821 agcggatgtt aaataagtat ccgctcaacc ctttatacct tacccttacg cttctgccct
     8881 taggcaacca catccgcgga tgggtcgctt tcctcaggcg agctgcttcg atctagcatc
     8941 tcgaggttga gctcgcgata ggtcaatgtc catgtcgatg acagatatgt ctcccgcaag
     9001 ccctccaaga agagcatcgg aatcgccagc acgaggatga acaggatccc tccggataga
     9061 acagccgcga tccaagcgac caaggtgcta gttgtcgcct ggaaaaccgt gtaaaagaga
     9121 aagcctacgc ctcctcccac aaatagaccc aatgcagcca agaggatagc cacaggtatt
     9181 aaggcgatga agaagccaac cttaataccc agcaagatca gccacatgat gaacacatcc
     9241 ttgaagttgc cgcgtattac ctccaacccc tggcggattg aagtaatggc atcgctaccc
     9301 tgcaagacgc ctactcgccg gatgatctcc agcaccagcg ataaaatcac gttaatgatg
     9361 attgccacaa agatgagcag aaaaaccaaa cccaccgagg caataatccc gggcattgtg
     9421 ggttgttgac ctgccagggt ttttaatagc acaggcaagg ctgcgcaacc aaaaatcagg
     9481 atgaacgcca aaaaaatggg caggtaaatc accaaatcga tcagaaacag ccgccaggct
     9541 gcccgtgacc aacccagccg cagacctgcc ttccaagtca atttctcctc atcctgctcg
     9601 tatttatcca ccatgcggat caacgccgcc tggctgacat aatatccgat ggcaaacagg
     9661 atggctaaga ggagcatagc caacacaaac aacacgccaa tgcgggtcaa cgtttccagg
     9721 gtatcagctg gaatgccttg attgaacaac tcacccagct tctccagctc gcgctggatc
     9781 tcctctggtg gggttgtctc atagcgacca ggaacattta aatcgggtga gaagttactg
     9841 ctccccgggg aaaaacctga tggcgaagtc aatgccaata taatccctat gacccagagt
     9901 gcccggtagc tccatagaat attccaggct cgcttgagag tttgcatgtg atccattttt
     9961 cgtactcctt tcaaatcaaa cttggcttac tgctgttcgc tgtttccaaa cccaccagcc
    10021 cactaaccag cagacataca tcaggctgat cagcagacct gcgccaattc cggtgaagaa
    10081 gcccccattg agcgggctga gtaaatccat catatttatc agggcgctta ggggctctgg
    10141 taaactcggc aataccgaga cgaaagatac ctgatctaac aagattaatt ttgcacctgg
    10201 caccacccac aacatgttgc ttagaatagc cactgtctgg aaaaaagtca aaaccaatag
    10261 cagaacaacc gggatgaagt accagcctag atcgggcacc atcgggatga tctgcagtgc
    10321 tttgcggcgt tgtttgagct gcaaaccaac ctccgctacg aagcgggctt cggatttaag
    10381 gttagccgga tcagggagtg ccataagaag cgcggataga tttttgcgct cttcgagcaa
    10441 ggcacgacaa cgctggcatt ctcccaggtg cgcttcgacc tgatgtctat gcagggaaga
    10501 aagttcatcg tctagataag cctcaatcca gatctccaga tgtccgttca tgttgcctcc
    10561 atcaatgggc gcaggatttc caccaatcgt gtgcgggcat aatgcagtcg agacataacc
    10621 gtgccgatag agatgtccaa tgtcgcagcg atttcacgat aggaaaggcc ttcatattca
    10681 cgcaagatca acaccacccg gctggcatgt ggcaggctaa gcaccgcttg gcgaatttca
    10741 cgggcacttt ctcgccgttc caggcggact tccaaatgct ctcctggact ggcaaggtcc
    10801 aagctatcga tatccacatt tggtttttca cggcgggtaa tatccagggc cgaatttaca
    10861 gcgatgcggt acagccagtt tcggaaggaa gagccgggac gaagatcagg caggtgttgc
    10921 caaacccgga tgaaagcctc ctgcgctgca tcctcagcaa ccctggcatc gccgcacatc
    10981 cggtagacca tgttgatcac tctcaagcga tagaggctaa ccaactcagc gaacgcttgg
    11041 cggtcgcctt cctgcgcgag agatatcagc ttgctttcat cccccaccga aacggcttct
    11101 agcatcatca tatctttccc aacccaacca accccaccat tctatttaac gaattttata
    11161 tcgattttat tcggattcag aaaaataaaa gttttttgaa aaagcgcctg gaaaatcccc
    11221 ttatccttgg ctttgttgca accactacct caatcaacag ccatagtggc tttccccaca
    11281 tcacatgcac ttttgcaaat tcgcggcgca gatattttag cagtggacaa tatcacctaa
    11341 tccaggatga tggattagct tcgctcgcag aaaacaaaat gggatctaag caggtgtttg
    11401 tgcgcccgca tcgatcccaa tataatcaag ttgtcattag aacaaatcag aacgccgaga
    11461 ttgcttcctg aagctctccg tagacctcaa tatatgaatc aaagccagcc atattgagca
    11521 ctttgagcac ctgcggtgat gagcatagta gcttgacata aggcgaaaca ggagttccgc
    11581 caaccgctgt gccagactta gcttcgccgc ccacctgcga tgcactttgc aagatattat
    11641 agatccagtg aatcgacctc agtcccacgc tgctcagcga agggaccttg ttgaggtcga
    11701 gaagaaaata gcgagctccc ttttcgtaag ccacgcggac tgcgttctct aatatatccg
    11761 tactgctcat gttgatccga ccagccacat gcagtatagt gatcggaacg cgaccctgta
    11821 cttcagattg cgaaatttcc aatctactgt tccaatccat aatccacatt cctccctatc
    11881 cataaatgca acttgtatta tggataagat tctcaccttg gtaatcattg ttgaaaagcg
    11941 attataggat acttgatatg cagccaggaa tcaagaggga atcacccatt tgcaccgcaa
    12001 tcacgggcag atcgacaaat actatcccgg ttaaccagaa caggcctaat ccaaagtgga
    12061 gaaaaacatc gaccagggag atataatcat gaaaagtata ctcaagattt tcccattaac
    12121 ttcaataatt tatcctacat acaaaaacat atcgtgctca cagagtacat tagggcaatc
    12181 gcatttgggc gattgcccat cactgaggag agaaaatggg gtgttttggt gggggcatac
    12241 ccccaccaaa acaccccaaa cactcttttc acgggattgc gcaggcccct cagggcatat
    12301 gcaattgccc tgcagagtac atagaaaaga ctcgccaacg gcgaaacatc tgatatgatt
    12361 aatttttgat agctacgagt gtacattttc gctgaagctt atgaatgttg gtaaatcacg
    12421 ccaatatacc gccgggttaa atgacctagc agaaatccgc cattttctcc aaaaacgggc
    12481 agccgaactt caggttgatc cttctacaac ctacgatctc ctcctggcgg taaccgagct
    12541 ggtcaccaac acgatgattt acggctatca gaatgagcca ggcttcatcg aagcagagat
    12601 gtatttagaa aaggattcgc tgatcatcca cctgcgcgat cacgcggcgc cctttaatcc
    12661 aacgcaattc ccaccgcctg atctttctat atcccttgag aaacgggcga ttggggggat
    12721 gggtatcttt ttagctaaac aattagtaga ccagatgata taccgagagt tacctcaggg
    12781 aggcaacgag gttactctgg tgatccatca aattagcaca agacaaaaag aggaaggaaa
    12841 cgatgaagat agaaattgaa tttaccaaag gaaaaatacc cgtcacgatc ctctctcttc
    12901 aaggagatct tgacgcctct aacttcgaat ctgtgatcgc cagggcgcgc gatctttata
    12961 ccgagggaac tcgtcatttg ttgctagact tgagtgagct gcgcttcatg agcagctccg
    13021 gcttggtagc catgcatagc attgtcttgt tgatgcgcgg taccgatcct cacgatctgc
    13081 tagaaggctg gaatgcaatc cacgccattg atcgggaccg cgcgagcgag cctcaatcct
    13141 atgtaaaaat cctcaacccc caacccaaag tagccaatac tcttaaaatg acagggctgg
    13201 accagttctt tgaaatctat caagaccgac aaaaagcgat cgcatcattt tagcaatgga
    13261 cacatcactc atccgccaag tgcccctgtt ttcaagcctg gagccatctg ggatcgagct
    13321 cctggctgca gcgctcaaag aatgccatta tccagccaag acagtccttt ttaaggaggg
    13381 agattacggc gataaattct atatcgtcgc cgaaggagaa gtggccatca tcaaggctat
    13441 cggcactccc aacgaacgac tgcttgcaat acgtgggagc ggtcagttca ttggcgagat
    13501 gagcctgctc aaccgcgatg ggctgcgcac agccagcgcc cgcgtgcact cggatgcccg
    13561 cctgcttgag ctgtcgcgtg aagatttcaa tgccttgtta gaccgagagc cgccgctagc
    13621 ttaccagatg ctgagcgtac tcagccagcg cctgcagact tcccagaacc aaacgatccg
    13681 cgatctttcg gaaaagaatc gccagttggc tgaggcctac gaaaatttaa aagccgccca
    13741 agagcaaatc atcgagaaga agatcctgga gcgcgagcta atgcaggcac gcgagatcca
    13801 ggaaagtatg ctacccacag ttttaccgcg cctgaaaggc ttcgacatcg tagcgcaaat
    13861 gctacccgcg cgtatggtgg gtggggattt cttcgatgtg atccgtctca gcccaaaaag
    13921 tctcgggatc gtcatcggtg atgtggctgg aaaaggtgtc cctgcggcgt tgttcatggc
    13981 actcacacgc agtctattgc gagcagaagc aaagcctttg gtctcaccag aaatcgtgct
    14041 acagaatgtg aaccataccc tgttggacat gaacgccaaa gggctcttcg tgaccgtatt
    14101 gtacggaatc ctgaaccagg aaacccgcga gtttaattac gtgcgcgccg ggcacgagtc
    14161 gcccatgctg tgggacaagc acggaaaaat tatcccaacc gtgttgggca aggggcaagt
    14221 gctgggtctg ttggatcatc cgatactcga gcccaggagc ctgaccatcc cactcggtgg
    14281 cgcgatgctg cttttctccg atggggtgac cgaatccgcc aaccagcagg aagaattttt
    14341 tggcacgcag ggcgtggagg ccatcatacc tgatctatta aacaaacctg tgcaagacca
    14401 gctcgatcac ttgctacagg aagtgagcca ccaccaaggt acaaacccac aggcagatga
    14461 catcacaatc atcgcggtac aagcgcacgc gtaacctacc agagcgataa atgatcaccg
    14521 tacaagatgt tcaggatgcc cgtcgacgga ttgaacctta tgtccgcaga accccattgg
    14581 aaaggaacga cactctcagt aaaaagctgg gaagtaatat ttaccttaag ttcgaactct
    14641 ttcaaaagac aggatccttc aaacctcgcg gcgcgttcaa ccagatccta caactaagcc
    14701 agcaggagag ggacaaaggc gctgtagcca tcagcggggg caattttgcc cagggtgtgg
    14761 catatgccgg gcaagtgctg ggcatgcgca cgataatctg catgccagat tacacgccca
    14821 ggaattacat ccaagccacg caagcctatg gagcagaagt gctgctctct cccaccttcg
    14881 ccgaaaccat tgagctagca caatcctacc aacgccgagg gtttagcttt ctgcacgcct
    14941 gggataatcc caaccagatg gcaggcaacg gcacgcttgg gctggagttg cttgaagacc
    15001 tgccagagct caccgacctg atcataagca ttgggggtgg cgggctgatt gccgggatca
    15061 tcgttgccat taaagcactc aagccgggtg tgcgcatctg gggtgttgag accgagggtg
    15121 ctgatacgat gggacaggct ttaaaagcag ggaaggtcgt gcaaatccaa ccgacctcct
    15181 tggctaaaac actgagtgct ccttatgttg cggaggatgc tctgctactc actcagaagc
    15241 atcttgaaaa gtatatcctg gtcacagatc gggaagcttt tgacgcccag ctctttttac
    15301 tggagcgcgc caaggtgatt accgaactgg ctgcctcatg cacactggca gctgccgaac
    15361 agattagcgg tgccttcgaa aagaataacc acgttatctt gctcttatgt gggggaaatg
    15421 cctcgctgga caacctgatg gaataccacc ggcaattgag aggcgaaccc gagccctaga
    15481 ccaataaccc aggctaagga taaattgcta tttcctggtt tgggggtctg actatgaatg
    15541 gctggttgga ttgatcgacc aaccttctaa ctcagcaatg gcttgctccg ttgtcatggc
    15601 atagcccccc gaccaggtag attcgaactc agcatcgctc atttgattac gaagcacaga
    15661 cagatagcgc tcgtattcca tccggtcatc gctccccatt cgagcacccg ttgaatcccg
    15721 cattttatcc gtcactgcaa acaggcgcgc cgcaagagat ggttgaccag tcatcgctgc
    15781 caatcctgcc aatgccccca agcaattgag aatgccctgc agattctcct gttcggctga
    15841 gaggatcaaa ctttccttaa aaaggctaaa agcctggtca ttgtgcccct cgctgcgctg
    15901 taataaaccc atgatttcaa gggaaaatgc tagaccccaa cggatatcca gttcacggga
    15961 aatggtcagg ctttcttcaa gcatttggga tgcagctgag ttatcacctt ggcggtaagc
    16021 cacccgtgca aggttgacca atacataagc tacatctttt cgatcaccga catcacggaa
    16081 aatatccagt gaactatgta acagtttcag cgcctccgtc aaatttccct tggcgtaggc
    16141 gatcaatcca agaaatgctt ttgaataagc aattccgcgc ggggcgccga tctcattggc
    16201 taaagtcagg gcttgggaag ccagcgattg agcaagatca taatcgccct gatggttggc
    16261 ggcatacaca cgcacctcaa gcgaaatgat catcccccgt ttgacaccta gctcacgaaa
    16321 gatctttaag ctttgctcag caagctctgc tccgcgagca tagtttccct ggtgaacagc
    16381 gatttcagcc aacgcggcga tgatgcgccc aattccacgc ctatcgccga cctcctgctt
    16441 taacaccagg cattcattaa gcagggttgt tgcctgctta aaatagccct gatcttttgc
    16501 aacagatcct aaattgagca gtgcgtttga gatacccagc ttatccttta gttcacggta
    16561 aagggataaa gcctcctcaa atagagaaat cgcgcgaaca aaatcgctta acagttgtga
    16621 aaccaaaccc aaattattca aagacagtgc agtagattgt ttatccccca gctcacggtt
    16681 caattttagg ccctcttcga gcagctcgcg ggcgtgtata tgctgccccc ggtcgagggc
    16741 tatcgtaccg agctgcccca atgacacggc gagaccttct ttgtcatcaa tttcatagcg
    16801 caacgccagg ctttctgtat gtaaggcgac cgcttcttga taattcccca agtaacgagc
    16861 gatctccccc aaatcattga gctcgcgagc aatacctaac ttgttcccca gttctcgaaa
    16921 gagagttagg ctttgttcat gtagcgcttt ggcttgcata taatcaccct gctggcaggc
    16981 gagaataccc gccccacgca gaccgtttcc ccgcagatag gctggcgcgt ccggattcgc
    17041 atccaaagcg cgcttcaacc aggcacgacc ctcgctgata tatccacgaa tttcccaaaa
    17101 gcgccataga gaactgccca agcgcaacag catttccgcc cggcttccat ccttcgacat
    17161 atattccagc gccatgcgca aattttcatg ctcagcttcc agctgcacca agcgtgtggc
    17221 ttgtcttgga ccccacagct cactatatgc ttcctcggct aggtttaaaa aatactccgc
    17281 gtgcttgcac gctagctcgt ccacttcttt ggcttctaaa agacgctcct gggcatactg
    17341 gcgaatcgtc tctaaatagc gatagcgctc actgcggtcg tgagattccg caacgaccag
    17401 cgatttgtcg atcagatggg ttagcagctc gagaatctgc caagcttcaa cttcttcatc
    17461 gaagcatacc ttttccgctg ctggcaatgt ccagccacca gtgaaaatcg atagtcgccg
    17521 cagtaaaatc tgctcgtgct cagatagcag ttcatgactc caatccaaca atgcacgcaa
    17581 ggtctgctgg cgagggagag cggtgcgatt gccgctgacc agcaatcgaa aacgatcatc
    17641 caggcgttca gcaatctctt caacggatag caccctcacc cgcgcggccg ccagctcgat
    17701 ggccagtggt atgccatcca accgtgcgca aatctttgcc accgagcgcg cattctcaac
    17761 cgtgatctcg aaatccgctt tgactgccac ggcgcgttcg acaaaaagtt tgactgcctc
    17821 gtactgaacc aggttttcag acttgactct ttccctcaag ccgggcgcag aaaggggcgg
    17881 aatccgccag atgacctcgc ccgacatgcc caacgcctcg cgactggtgg ccagtatccg
    17941 caattcaggc gcactgcgca gaatcgtctc aacgaaaagg gcgacaccct ggatcaggtg
    18001 ctcacaatta tccagtacca gcagggtatt cttggtacgc atataactgg ttagcaactg
    18061 gagcaatgac ttacctgctt cttcgcggat gcctaaaaca tctgctacaa actggatgat
    18121 tagttcaggg ttggatatcg gtgccagatc catccaccac acaccatcac caaagaattc
    18181 cagaaggtct tcggccacct gcaatgctac gcgcgttttg cccgccccgc caacacccgc
    18241 gatggtgagc agtcgcgttt gcgtcagtaa ttgtttcact tcttctatct cacgttcacg
    18301 accgacaaag ctggtaagtg cggcgggtaa attggtatgc aggttctcca acgctttgag
    18361 gggtgggaaa tctcccggca atccctcgat aaccaattgg taaatgtgct ctgttcgatt
    18421 aagatctttt aggcggtatt cgcctaaatc gcgcatactt accccctccg gtaaagcatc
    18481 tcgtaccaga acattggtgt tctgggatac aagcacctgt cccccacgag cagcgtttcg
    18541 gagtctggcg catcgattca cggctgggcc gtaataatcg ttgtcgcgca aatctgccgc
    18601 accagtatgc aatcccatgc gcacgcgcag gggtgagggg attatccaag cttcgaaaga
    18661 cagagcacgc tgaatagcac tggcagcagc aacagcatcc gcagcgcgag gaaacacggc
    18721 aaaacggcta tcgccttcgc cacgcggccg gacgacagaa ccaccataat tcgtcaccaa
    18781 cgactcgatc aagtcatcgt gacgaatcat caccccacgc atttggtctg gatattgctc
    18841 ccaaaggcgt gtactacctt ctacatcggt aaaaagatag gttagcgttc ccgtgggcaa
    18901 agaagaagat gtggacgggt atcgctcagc gatgggggac atcgttgtct aacagggcac
    18961 ccttttcctt tataagattg gtcaaacgca ctacttcctg gctttgctcg cgtatgcgcc
    19021 gcgacatggt ctgaatcatg cggtatgcga gtgaggggtc ctcgtggatt cgttggagaa
    19081 tatttttctt gtccactgtc aatacgcgaa cctcacccag cgcccgaaca gtcgccatgc
    19141 gcacctcacg ttcaaaaatc gccatctcgc caaaaaattc cccctcaccg cgcacagcca
    19201 ggcgaatttc ttttccgtct ttttcactca ggatttcaac ctgcccttgc tggatgacaa
    19261 acatacaatc ccccgcctca ccctgtcgga tgatcagctc cctatcctga tatacttttc
    19321 ccaattcacc tgttttcatc attccctcat ctcacgatca ggaaggtgtt gctggttctc
    19381 tagcaacacg aattaattgt acgccatcag ccaaatgatt gtacagccgc aacgaagaaa
    19441 tgcttcaata gacgcatcca aaaaacaggg tgcaacgtgc gcaagaggat ctttcgatat
    19501 ggcgcgctac cggtcagcat atcccacatc accatactca tgttgcggtc tccgaccacc
    19561 ccgcgctttt gctcgcttaa taccatctgc aaaatcgctc gccgggcaaa tttccgtttc
    19621 tgaacttccc gaacaacagc aaacagaatt tttccgatgg tattatcgat ttccatcgac
    19681 cggcaagcag gccaataacg ccgttcgaaa gcttcagacg atacgccctc gaaaatcgcc
    19741 gccgtcgccg cagctttcgc cgcgcggtaa gctgccccaa taccatcttt gtacaaacgg
    19801 ctcacagcag aatcgccaat gaagacaatc cggtctccat aaggtcgggc actaccctta
    19861 atattaatgc gtggcgagca ccaacaatcg acctgatcca tagggtagtt gggaggcata
    19921 cagcgctcta gtgctggatc gctcgtaaag gtttgaagaa gatctgcatc aatattctca
    19981 cccaacaaag caagagaggc gtaatcacct ttagggacca tggcagcaaa atctagattg
    20041 ggaatgttca gcaggaaagc gtgaaacgat gagcctaaat gcttcgatac ggcttcttca
    20101 cccaagtaat actcacgtac aaagcactgc gttgtgagag ggggttggta gccaaaatcc
    20161 ataccctgga agagtttaag gatggcggta ttcaccccgg cagaaacagc cagcaggtca
    20221 taagtttgtg tcaatccttt ggtggttttc acctgaagtc gctgaccacc tccgtttaac
    20281 ttttcgggat cagcgacttt ctcgacttcg cttacccggg cggggatgtg ctgggcaccc
    20341 ttgtccaaag ccagcttcat caaaaagtga tccagactgt cccacttgat ttccttaatc
    20401 cctcgcgggc caacgcctcg aaacgcagtc ccgatgcgtc tctcatcacg aatggagggg
    20461 atgagtgcgc tgcccacatc catgtgcagc acataggagt caatcccgcg ctgcaccacc
    20521 gtggacggca gaattattcc ctcggttgcc aaattctgga caagtgactc agacaccacg
    20581 ccagcacaca tattgcagcc ggtcggacca ggtttggaat aatccctggg ctcgtaaata
    20641 tccacaaaga tctccaaacc catgcgttct gcgaattgca aaaggaagta actaaaaaaa
    20701 gagcctgctg gtcccccacc aatcaccgca acgcgggatc cgtccttcaa cttgagattg
    20761 ttgctaccgt ccatgtctat cgctcccttt accgcaacca tacttcatta tgccatgaac
    20821 ttaagcatct gagaaagttt aattaatcaa cttctcaaaa tcgttagtag aagtattgca
    20881 aatagaatgg acacttccca ctttcgaaac caccatgaca cgtaatcggg atacaggtct
    20941 tgaacaagga attagataag tgtaccatct tggatttagc cgggtttcta caagagcttc
    21001 cattgagatt attctggttg caatactcaa taattatagg acttacgcag ttgaagagga
    21061 aaggaggggg
//
LOCUS       DBNGSX010000058         5588 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_903960, whole genome shotgun sequence.
ACCESSION   DBNGSX010000058 DBNGSX010000000
VERSION     DBNGSX010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5588)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_903960"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="AC16CB_01915"
     CDS             complement(<1..196)
                     /locus_tag="AC16CB_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007162635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HIR3341251.1"
                     /translation="MSLQLSSSDQDMLAGAKGPAAKLAMSILARMAEVYGATELMDIS
                     AAHIDSTIYIGEAGLEFAERL"
     gene            complement(262..2103)
                     /gene="glmS"
                     /locus_tag="AC16CB_01920"
     CDS             complement(262..2103)
                     /gene="glmS"
                     /locus_tag="AC16CB_01920"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 -
                     L-glutamine:D-fructose-6-phosphate transaminase
                     (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR3341252.1"
                     /translation="MCGIVGYIGSRDATPIILNGLKRLEYRGYDSAGLAVLENGHIEV
                     RRDAGKLARLSAMVDDSPVHGNLGIGHTRWATHGEPSARNAHPHLGMTGNVVVVHNGI
                     VENFLELREELSAEGVEFKSDTDTEVIVHLVERYLSRDTGLAEAARSALSHLKGAHGV
                     VIFSSQEPDKIVAARVGNAGGVVVGVGEGEMFVASDMPAILEHTRRMVFLESRQMVVV
                     TRDGLEVKTLDGAPVNYEMHSVAWDPVAAEKGEYRHFMQKEIHEQVRSLTDTIGGRAD
                     FEEGRILLPELNLTPEFAQRLRKIFIIACGTAAHAGMVGKVLIEHIARIPVEVDIGSE
                     FRYRDPIIDQDTVVLAISQSGETADTLAAMEEARRKGATLWSIVNVIGSQAMRIADGS
                     VSMQTGPEIGVASTKAYTAPLVDLYMLAILLADLRGVISERERRRLVNDLRLVPDLAG
                     QCLDREQEVESVARALLHVNHCLYLGRGINMPTAYEGALKLKEISYIHAEGYPAGEMK
                     HGPIALIDKDMPVVVIAPRDPWYEKMLSQIEQAKARGGVVVALATDGDSLIPGLADYV
                     LWLPEAPWLLSPVTAVIPLQILAYHIAALRGLDVDQPRNLAKSVTVE"
     gene            2721..3791
                     /gene="mreB"
                     /locus_tag="AC16CB_01925"
     CDS             2721..3791
                     /gene="mreB"
                     /locus_tag="AC16CB_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HIR3341253.1"
                     /translation="MAFNPLNWLLGFFSLDIGIDLGTANTLVNVRGKGIVINEPSWVA
                     VEKRSRKPLAIGAEAKEMVGRTPANVIAIRPLRDGVISEFEITQAMLEYFIGKAHEQS
                     IVPVPRPRVVIGIPSGVTEVEKRAVYDASMSAGARETYMIEEPVAAALGAGLPVSEIR
                     GSMIVDIGGGTSEVAVMSMGGVVVSRSLRVAGDEMDEEIVQYMRNKYNLTIGLRMAEQ
                     AKISIGSAYHLPEEKTMLLRGRNLVSGLPESVEVSSVEVREAISGSVQTIIDTVKDAL
                     DEAPPEIIADLMDTGVCLAGGGALLQALDRRLTEDLRIRVWVAEDPMSCVARGAGMIL
                     EDLDHLNHLLVGLERGSTRHSQ"
     gene            3851..4705
                     /gene="mreC"
                     /locus_tag="AC16CB_01930"
     CDS             3851..4705
                     /gene="mreC"
                     /locus_tag="AC16CB_01930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR3341254.1"
                     /translation="MNTPSDRPRSLQTVIFILIAVGLIALALGGYLTPLSRILLNPMV
                     AVQTWVATRFQAVQDIVSAPQDTTRLRQRNAELEAEVARLQTQIIELQEQLAETNVLS
                     ALVDFARANPENRYQAAAVIARDPSPFLQYVIINRGSDDSLRRGMPVVTQQGLIGRIA
                     AVTAGAARVQLITDPASTVNVRLEPSGAQAILQGQITGEILLDMIPQGANVQPGDLVL
                     TSGLGGGYPANILIGQISSIRSRDQDIFQRASIQTVLDFSRINIVLVIVNFKPIDINP
                     LLPTPVAP"
     gene            4740..5240
                     /locus_tag="AC16CB_01935"
     CDS             4740..5240
                     /locus_tag="AC16CB_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341255.1"
                     /translation="MATFIAIPILSGLMILQSGIISRIPLLQGTPDLLMLFILGWAMQ
                     KRVQTAWQWCIIGALIFGLASALPLGTPLLGYGLATGVALLLRRQLWQIPLLAMLVAV
                     LVGTFANHAISLIALRLVGNPIPIPQALNLITLPSAFLNLIFAIPAFAMMGDLANWLY
                     PEALEA"
     gene            5237..>5588
                     /locus_tag="AC16CB_01940"
     CDS             5237..>5588
                     /locus_tag="AC16CB_01940"
                     /inference="COORDINATES: protein motif:HMM:NF015662.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341256.1"
                     /translation="MNESPALHNQVNPWRIWVFALIFLLVLGVYIFRLFTLQIIQGPV
                     WLAQATENRTRAINLPTERGVIYDRNGVVLARNIASYNVVITAANLPDDPGEIQEIFR
                     ALSAQIDVPINLNEI"
BASE COUNT         1260 a         1620 c         1441 g         1267 t
ORIGIN      
        1 ccagccgctc tgcaaactcc agcccggcct cgccgatata gatcgtgctg tcgatatggg
       61 ctgcgctgat atccatcagc tcggtcgcgc cgtacacctc agccatgcgc gccaggatgg
      121 acatggcaag cttggctgcc gggcctttgg cacctgccag catatcctga tcggatgaag
      181 aaagctgcag agacattaat cccaactttt tgaaagacaa gcggcgtacc aggatacgcc
      241 ttgatcgctg gttaagaact tttattccac ggtgacgctt ttcgccagat tcctgggttg
      301 gtccacgtcc agaccacgca gggcggcaat gtgataagcc aatatttgca gcgggatgac
      361 agctgtcacc gggctgagca accagggtgc ctcgggtaac cacagcacat agtcagctaa
      421 accagggatc aacgagtcgc catccgttgc cagagcaacc accaccccgc cgcgcgcctt
      481 ggcttgctca atctggctca acattttctc ataccaggga tcgcgcgggg caatcaccac
      541 aaccggcata tctttatcga ttaaggcgat tggaccatgc ttcatctcgc ccgccgggta
      601 tccctcagca tgaatgtacg aaatctcttt gagcttgagc gccccttcgt atgcagtggg
      661 catattgatc ccccggccca gatacaggca atggttgaca tgcagcaagg cgcgagcaac
      721 gctctccacc tcctgctcgc gatccaggca ttgcccagcc aaatcgggca ccaggcgcaa
      781 atcatttacc aagcgccggc gctcacgctc gctgatcaca ccgcgcaagt ctgccagcaa
      841 gatcgccagc atgtacaggt ccaccaaggg cgccgtatag gctttggtag aagccacacc
      901 gatctcggga ccggtttgca tagagacaga cccgtcggct atgcgcatcg cttgagagcc
      961 aataacgttc acgatggacc acaaggtggc tcctttacgg cgcgcctcct ccatggctgc
     1021 cagcgtgtcc gccgtctcgc ccgactgact gatcgccagc accacagtat cctggtcgat
     1081 aattgggtcg cggtagcgga actcggagcc aatatcaacc tccaccggta ttcgggcgat
     1141 gtgctcgatt agcacttttc ctaccatgcc ggcgtgggca gccgtaccgc aggcgatgat
     1201 gaagatttta cgcagacgtt gagcgaactc tggggtcagg ttgagctcgg gcagcagaat
     1261 gcgcccctct tcaaaatctg cgcgcccgcc gatggtatcc gtgagcgaac gcacctgctc
     1321 gtggatctct ttttgcatga agtgacggta ttcgcctttt tcggccgcca ctggatccca
     1381 ggctacgctg tgcatttcat agttcaccgg cgcgccgtcc agtgtcttca cctccaagcc
     1441 gtctcgagtg accaccacca tctgccgcga ctcaaggaac accatgcggc gggtgtgctc
     1501 caagatggcg ggcatatctg aggccacaaa catctctccc tcacccacac ctaccaccac
     1561 cccgcccgca ttaccaactc gagcggccac aatcttgtca ggttcctgtg atgaaaaaat
     1621 gaccacaccg tgtgcgcctt tcaagtgaga gagtgcgcta cgggctgcct ccgccaggcc
     1681 tgtatctcgg ctgagataac gctcgaccag gtgaacgatc acttccgtat ctgtatccga
     1741 tttaaattct actccctcgg cactgagctc ttcgcgcagc tccaggaaat tctccacaat
     1801 cccattgtgc acgaccacta cattgccggt catgcctaga tggggatggg cattgcgggc
     1861 acttggctcg ccatgcgtag cccagcgcgt gtgcccaata ccaaggtttc catgaacagg
     1921 cgaatcatcg accatcgccg aaaggcgcgc cagcttgcct gcgtcgcggc gcacctcgat
     1981 atgcccgttc tccaacaccg ccagtccagc ggagtcataa ccgcggtatt ccaaacgttt
     2041 caacccattc agaatgatgg gcgtggcgtc ccgcgatcca atgtatccaa cgattccaca
     2101 catgggattc cctccaaatt tcttttagat tctgccactc ttcacggttt tgtctgtgcg
     2161 gttgcccggg cagcgtttgc cgcgaagttt acaatggagg gaaagaaagc gggccgtggc
     2221 tggcccggag ggcttccgct gatcattcga ttttcccggc cctcaggctg gcaacctaaa
     2281 gggtagccgg ttaccccgcc ctcgcaggcg ggaaccctcg tcctcgtcaa ccttctgtaa
     2341 gaaggtcctt gcgctatctc tccctagaat aattgcttat aaaggccact tacctcctaa
     2401 atatagatca taacgcgcga aaattatacc tgattcctaa tccctaagac atggttttca
     2461 tgattgtaag ctaaatagta ccccacgacc gtctgacatt atgtacaaaa cagaaatggt
     2521 atggaaattg gtaaaacgac atgcccccaa gccataaatg acatgttttt atggaaaatg
     2581 tgagaatttt aacaatgtaa atactgaaat tcggtggggg tcatgttctg gtataatcca
     2641 tcccgatgtg tctgagaggg ccattcttaa caatgcctta aagagataca catccatagc
     2701 gaacgacgga ggagcctgaa ttggcgttca atcccctcaa ctggctgttg gggtttttct
     2761 ctctggacat tggcatcgac cttggcacag cgaacacatt agttaatgtc cggggtaaag
     2821 gcatcgttat taatgaaccg tcttgggtag cggtcgaaaa gcgcagccga aaaccgttag
     2881 ccatcggcgc agaggcgaag gaaatggtgg gacgcacccc agccaacgtg atcgctatcc
     2941 gaccactgcg cgatggagtg atctccgaat ttgagattac ccaagccatg ctggaatact
     3001 ttattggtaa agcccacgaa caaagcatcg tgccagtccc tcgaccgcgt gtagtcattg
     3061 ggatcccgtc tggcgttacg gaagtggaga aacgggcagt gtacgacgca tcgatgtctg
     3121 ccggagccag agaaacctat atgattgaag aaccagtggc agcggctctg ggcgctgggc
     3181 tcccggtgag cgaaattcgt ggtagtatga ttgtagatat tggcggcggc acttctgagg
     3241 tagccgtgat gtcgatgggc ggtgtggttg tctcccgctc gttacgcgtg gctggcgacg
     3301 aaatggatga agagattgtt cagtatatgc gcaacaagta caatttaacc attggtttgc
     3361 gcatggccga acaagccaag atatcgattg gctcagcgta tcacctgcca gaagagaaga
     3421 caatgcttct acgtgggcgt aacctggtaa gcggattacc cgaatcggta gaagtttcgt
     3481 ctgtggaagt ccgcgaggcg atctctggct cagtgcagac cattatcgat actgtgaaag
     3541 atgccttgga tgaggctccg ccagagatca ttgccgacct gatggatacc ggtgtctgcc
     3601 tggcgggtgg tggtgctcta ttgcaagccc tagatcgccg cctgacggag gatctccgca
     3661 tcagggtctg ggtcgcagaa gaccccatga gctgcgtggc gcgcggcgct gggatgatcc
     3721 tggaagactt agaccacctg aatcatttat tggttggatt agaacgtgga agcacaagac
     3781 atagtcaata gaaaatacct ggcgattcta tctggcatat cctacctgac ggaatcacca
     3841 atgctttaac atgaacaccc catcagatcg tcctcgctca ctgcaaaccg tgatctttat
     3901 cctgattgca gtggggttga ttgctcttgc gctgggcggc taccttaccc ctctttcccg
     3961 catcttactc aatcccatgg tcgctgtcca aacctgggtc gcgacgcgct ttcaagctgt
     4021 tcaggatatt gtcagcgcgc cacaagacac tacccgcctt cgccagcgca atgcagagct
     4081 ggaggctgag gtagcccgct tgcagacaca gatcatcgag ttgcaggagc aactggctga
     4141 aaccaatgtg ctctctgcgc tagtggattt cgctcgcgcc aaccctgaaa accgctatca
     4201 agccgcagca gtgattgccc gcgaccccag ccccttcctg cagtatgtaa tcatcaatcg
     4261 cggctccgat gacagcttgc ggcggggcat gcctgtggtg acacaacaag ggttgatcgg
     4321 gcgcatcgct gccgtcacag ccggtgcagc gcgtgtccag ctcatcaccg atcctgcctc
     4381 cacagtcaac gttcgcctgg aaccatccgg agcccaagct atcttgcaag ggcagatcac
     4441 cggtgagatc ttgttggaca tgatccccca aggagccaat gtacaaccgg gcgacttggt
     4501 gctcacttcc ggcttgggag gcggttaccc ggcaaacatc ctcatcggac agatctccag
     4561 cattcgcagc cgggaccaag acatcttcca acgcgcttcc atccagacag ttctggattt
     4621 ctcgcggatt aacattgtcc tggtgatcgt caacttcaag ccgatcgaca tcaacccgct
     4681 cctgcccaca cccgtagcac cgtaatcttt agaatctggc ttaaaatagt gtcagtgtca
     4741 tggcaacatt tatcgcaata cctatcctta gtggattaat gatcctgcaa agcggaatta
     4801 tcagccgcat cccgctgctc caaggcaccc ctgacctgct tatgcttttc atcctggggt
     4861 gggcaatgca aaaacgcgtc caaactgcct ggcagtggtg catcatcggc gcgctgattt
     4921 tcggcctggc ctcggctttg cccttgggta cgcccctatt aggatatgga ctggcaaccg
     4981 gcgtggcgct gctattgcgc aggcaattat ggcagattcc tctactggcg atgctggtgg
     5041 cagttctggt gggcacattt gccaaccatg ccatttcctt gatcgctttg cgactggtag
     5101 gaaatcccat tcccatcccc caggcgctga acctgatcac gctgccgagt gcatttctaa
     5161 accttatctt tgctattcca gccttcgcca tgatgggtga tctggcgaac tggctttatc
     5221 ctgaggcatt agaggcatga acgaatcccc tgccctgcac aaccaagtga acccctggcg
     5281 catctgggtt ttcgcgctaa tctttttgct ggtgctggga gtgtacatct tccgactctt
     5341 cacactgcaa atcatccagg gcccggtatg gttggcgcag gcgacagaga atcgcacccg
     5401 agcgatcaac cttccaaccg aacgtggtgt gatatacgac cgcaacggtg ttgtgctggc
     5461 gcgcaacatc gcttcctaca atgttgtgat tacagctgca aacctgcctg acgaccccgg
     5521 cgaaatccaa gagattttcc gcgcgctttc ggcgcaaatc gatgtcccaa ttaatctaaa
     5581 cgagatca
//
LOCUS       DBNGSX010000059         4884 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1181321, whole genome shotgun sequence.
ACCESSION   DBNGSX010000059 DBNGSX010000000
VERSION     DBNGSX010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1181321"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /gene="upp"
                     /locus_tag="AC16CB_01945"
     CDS             <1..398
                     /gene="upp"
                     /locus_tag="AC16CB_01945"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIR3341257.1"
                     /translation="RAGLGMVEGVWNLMPAAEVWHIGLYRDERTLKPVEYYNKLPIEP
                     TVAMCLILDPMLATGGSAVATVDVLKRWGVRKIKYVGIIGAPEGIRLMSEHHPEVPIH
                     LAAVDERLNEHGYILPGLGDAGDRQFGTG"
     gene            complement(414..1034)
                     /locus_tag="AC16CB_01950"
     CDS             complement(414..1034)
                     /locus_tag="AC16CB_01950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015078190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR3341258.1"
                     /translation="MKQKHFIDSHKSVTFIYILTMIVLYKQWENPTAWVYLALHGTYG
                     ILWVLKGRIFPDRQWEQPTGWLYGLVIWGGLSLYWVAPWLLMARGVQAPAWYLGLCIS
                     LNIFGVFFHFVADMQKYTTLSLQPNRLFTGGVFSKIRSINYFGEMLIYFGFSLLAMHW
                     LPVVILLVWIIFVWLPFMRKKEHRLSRYPEFAAYKNRTKMFIPFLF"
     gene            1266..3494
                     /locus_tag="AC16CB_01955"
     CDS             1266..3494
                     /locus_tag="AC16CB_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3341259.1"
                     /translation="MSTDKIGRYIIKAELGRGGMSTVFHAYDPSFERDVAIKVLPAAF
                     LHDPQFRARFDREAKMIANLEHPAIVPVYDFGEQDGLPYIVMRYMAGGSLADRLAKGA
                     LSFEETSRIISRLAPALDAAHARGIIHRDLKPGNILFDQYSNAFLSDFGIARLTESSA
                     TLITGESIVGTPAYMSPEQVQSNKTIDRHSDIYAMGVLTFQMLTGQAPYQAESPAKVM
                     MMHLLEPVPTILDKKNDLPQGTEEIISRSMAKEPADRYSTTAELASALEALVHGSQAE
                     IQIQPVAPRGLSTSGSVRGAAKPIKQPSPVVLPDESLATTPGLPGVQSVATTQPTRRT
                     LTLPLVLIIILLVGGIGAMSVFLFSGRQGSSFPLLGAVQASATSASTNTPVPPTQTTA
                     PQPTSTIPVLMLDTPSAEPSPTPMATPSPTITATSQPTTLVLGGADKLAFLSGSNVWA
                     ANLDGSELVQLTDDGANKTSLQWTPDGQALIYVSGKCVQMVSLDGATIENLTCINYID
                     SLKSFDISPDGKQTAITVDNQLYIVPFDMARLKEATTRSDLTEMAECKDFAPYQKNPV
                     TQVRWSKDGTTVAAKLMADVGGQRGNIVQLFKVDYCAPNPRPIDNFPPPRFTMSGYNK
                     NPFILNFGWDGVVLFALNNYVRNDGFGDLYLYNSEIHKVDEKANPVNNICCYRDPHFS
                     PDGSYLLFSFQNLNEGTGSKTWLYYIPVASIGTGASYEPLPLPPIDDPRESPWPVLRP
                     AK"
     gene            3581..4450
                     /locus_tag="AC16CB_01960"
     CDS             3581..4450
                     /locus_tag="AC16CB_01960"
                     /inference="COORDINATES: protein motif:HMM:NF048886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkZ-related protein"
                     /protein_id="HIR3341260.1"
                     /translation="MTSIPYELLLSYRNETYRLLPGKRISNRDEAVEFVNQRGFIYFW
                     PIRDTLLPSLWTAVAGDRPVADAHDDPGHVTWGWKDSLIGKNEWYYAKVLRRKATIIS
                     MSTAPFFYALSENYGSPEDDYLILYEQGHLTQEAKAVYQAILENGPLDTIALRRAARL
                     SSRESDSRFNKALADLQAGFLLVPVAVTQAGAWRYAFAYDIVARHHPEIVEKARYIGE
                     LDARRKLVELYFRSVGGAQLRDVNMLFRWKPADVSSTIDYWVENQLICRVEIEGQSGE
                     WLILQDLFLEHRE"
     gene            4461..>4884
                     /locus_tag="AC16CB_01965"
     CDS             4461..>4884
                     /locus_tag="AC16CB_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341261.1"
                     /translation="MNKNPFPSLPFPSLVMHADWSVTPAKRWIARATLGADGRYHAEQ
                     SQRVEHATSLLSTLGEQVGQAGAALVGFDFPIGLPLAYARRAGIKEFITILPELGKDR
                     WSKFYEPAHTPEEISIRRPFYPARAGNTRQQHLLKQLGF"
BASE COUNT         1276 a         1337 c         1179 g         1092 t
ORIGIN      
        1 tgcgggcggg gcttggcatg gtcgaagggg tttggaacct gatgccagca gctgaggtct
       61 ggcacatcgg cctctatcgt gacgaacgca cactcaagcc tgtagagtac tacaacaaac
      121 tgccgataga gccaaccgta gcaatgtgtt taattcttga tcccatgctg gcaacgggtg
      181 gttcggctgt cgctacggtg gatgtcctca agcgatgggg ggttcgcaag atcaagtatg
      241 ttggcatcat cggcgctcca gaaggcatcc gactgatgag cgaacaccac ccagaagttc
      301 ccatccacct ggcagcagtc gatgagcgcc taaatgagca tggctatatt ctgcccggtt
      361 taggcgatgc cggcgacagg caattcggaa cgggctaaga aacgctcagc ttttcagaac
      421 aagaagggga taaacatctt ggtgcgattt ttatacgctg caaattcggg gtagcgcgaa
      481 agacggtgtt cttttttgcg cataaatggc aaccagacga agatgatcca aaccaacaag
      541 atcactaccg gtaaccagtg catcgccagc aagctaaacc cgaaatagat caacatctcg
      601 ccaaaataat tgatactgcg tatttttgaa aacacacctc cggtgaacag gcgattgggc
      661 tgcaagctca aagtggtata tttttgcata tcagcgacaa agtgaaagaa cacgccaaag
      721 atattcaggc tgatgcaaag acccaggtac catgctggag cctgcacacc gcgagccatc
      781 agcagccagg gtgccaccca gtacaagctc agccctcccc agatcacaag accatacagc
      841 cagccggtag gttgctccca ttgccgatcg gggaagattc ggcctttcaa tacccacagt
      901 atgccgtatg tcccatgcaa ggcaaggtaa acccacgcag tgggattctc ccattgcttg
      961 tatagtacga tcatggttaa tatatagatg aatgtaactg atttgtgcga atcgataaaa
     1021 tgtttttgct tcaaaatagc accttttctg tcaattatgc aaggtcgagt ggcgtaactt
     1081 gcacaaccac taccattgac gtacaattcg aaaatagaac acatccacta ggttgtgaac
     1141 caacgcgacg ttagcattat atccaaataa aggcttctct tccccaaaaa taaaaagcag
     1201 tctcccaggt ttagtaaaac tggggctctg catctaataa cggaaatctc acagaagcaa
     1261 aatcaatgtc aaccgacaaa atcggacgat acatcatcaa agcagagctg gggcgtggcg
     1321 ggatgtcaac cgtatttcac gcctacgacc cgagcttcga acgcgacgta gccattaaag
     1381 tcttacctgc ggcttttctt cacgatccgc agtttcgagc ccgcttcgac cgcgaggcga
     1441 agatgattgc taacctcgag cacccggcga ttgtgcccgt ttacgatttt ggtgagcaag
     1501 atggcctacc ttacatcgtc atgcgctaca tggctggcgg atcactggca gacaggttag
     1561 ccaaaggggc tctgtcgttt gaggagacca gccggatcat atcccgctta gcccctgcac
     1621 tggatgcagc acatgcgcgc ggcatcattc accgcgatct caagccaggc aatatcctct
     1681 tcgaccagta cagcaatgca tttctttcgg atttcggcat tgcccgctta acggaatcct
     1741 cagccacact gatcactggt gagagcattg ttggcacgcc ggcttacatg agcccagagc
     1801 aagtgcagag caataaaact atcgacaggc atagcgacat ctatgcgatg ggtgtgctca
     1861 ccttccagat gctcaccggg caagctccat accaggcgga gtcccctgca aaagtaatga
     1921 tgatgcacct tttggagcca gtccccacca tcctggacaa aaaaaacgac ctgccacagg
     1981 gaactgagga gatcatctcc cgttctatgg caaaagaacc ggcagaccgt tattctacaa
     2041 cagctgagct ggcttctgcc ctggaggcat tggtacatgg ctcgcaggca gagatccaaa
     2101 tccagcctgt ggctccacgc ggcttgtcca cttctgggag tgtgaggggg gcagcaaaac
     2161 caatcaaaca accatcccct gtggttctgc cagacgaatc cctggcaact acaccaggat
     2221 tacccggcgt tcaatcggta gccacaacgc aaccaacccg tagaactctt actctaccgc
     2281 tagttctcat tatcatcctg ctcgtgggtg gtattggcgc gatgagcgtt ttcttattct
     2341 ctgggcgcca gggtagttca tttcctctat tgggtgcagt acaggctagc gcaacctctg
     2401 catccacaaa tacgcccgtc cctcccaccc aaaccactgc gcctcaaccc accagcacca
     2461 ttccagtact gatgctggat acgccctcag cagagccatc gcccacccca atggcaacgc
     2521 cttcccccac catcacagcc acatctcaac ctaccacact ggttcttggc ggagccgata
     2581 agctggcttt ccttagtgga tccaacgtat gggcggctaa cctggacgga agcgagctag
     2641 tgcaactgac cgatgacgga gcaaacaaaa ccagtttgca gtggacgcca gatggccagg
     2701 ccctaatcta tgtttccggt aaatgcgtgc aaatggtctc gctggatgga gcgacaattg
     2761 aaaacttaac ctgcatcaat tacatcgatt ccctaaaatc cttcgacatc tccccggatg
     2821 gcaaacaaac cgcgatcacc gtggataacc agctgtacat tgtcccgttc gatatggctc
     2881 gcctcaaaga ggcgacaacg cgtagtgacc tgaccgaaat ggccgaatgc aaagacttcg
     2941 ccccatatca aaaaaatcct gtcacgcagg tacgctggtc gaaagacggc actaccgtag
     3001 cagccaaatt aatggccgac gtgggtggcc agagaggcaa cattgtccag cttttcaaag
     3061 tggattattg cgcccccaat cctcgcccta tagataattt cccacctcca cgcttcacga
     3121 tgtctggtta taacaaaaat ccatttattt tgaatttcgg atgggacggt gtcgtcttgt
     3181 ttgcattaaa taactacgtc cgcaatgatg gcttcggaga cttgtacctc tacaatagcg
     3241 agatccataa agtcgatgaa aaagccaacc ctgtaaacaa tatttgttgc taccgcgatc
     3301 cgcacttcag tccggatggt agctacctgc tcttttcttt ccaaaactta aacgagggga
     3361 caggcagcaa gacctggctt tattacatcc cggttgccag catcgggact ggtgcaagct
     3421 acgagccatt accgctcccg cccatcgatg acccacgcga aagtccctgg ccggtactga
     3481 gaccagcaaa atagaggctt ggcagatgat aggacaaacg acgaagggag atcttgagcc
     3541 ttcgtcgttt gcctattgcc aatggattta taatcattgc atgacctcca tcccctatga
     3601 gcttctgctc tcttaccgga atgaaaccta tcgcctattg ccaggcaagc gcatctccaa
     3661 tcgggatgaa gccgtcgaat ttgtcaacca gcgcggcttc atatatttct ggcctatacg
     3721 agacacattg ctccccagct tatggacagc tgttgccggc gatcgaccgg tcgcagatgc
     3781 ccatgatgac cccgggcacg tcacctgggg atggaaagac tcgctgatcg gcaagaacga
     3841 gtggtattac gcaaaagtct tgcgcaggaa ggccaccatc atctcaatga gtacagcgcc
     3901 ctttttttac gctctatcgg agaactatgg ctcacctgaa gatgattacc tcatcctgta
     3961 cgagcaaggc cacttgactc aggaagccaa agcagtttac caggccattt tagagaacgg
     4021 cccactagac accatagccc tgcgccgagc ggcacgcttg agcagccgag aaagtgactc
     4081 ccgattcaat aaggcacttg cagacctgca ggcaggcttc ttgctcgttc cggtggcggt
     4141 tacgcaggct ggagcatggc gctatgcctt cgcctatgac atcgttgccc ggcatcatcc
     4201 ggaaatagta gaaaaagcgc gctacatcgg cgagctggat gcgcgccgca agctggtcga
     4261 attgtatttc cgatcagtgg gaggagcgca gttaagagat gtaaacatgc tcttccgatg
     4321 gaaacctgcc gatgttagct ctaccatcga ttattgggtc gaaaatcaat taatctgtag
     4381 agtggaaatt gaaggccaat caggcgagtg gctcatttta caagacctgt ttctggagca
     4441 tagagagtaa gaatttctga ttgaataaaa atccttttcc ttcgttacct tttccttccc
     4501 tcgtcatgca tgcagactgg agcgttaccc cagccaagcg ctggatagct cgcgctaccc
     4561 tcggcgcgga tggtcgctac catgccgaac agtcacaacg agttgaacat gctacttcat
     4621 tgctcagcac attgggagag caggtggggc aggctggtgc tgcgctggta ggtttcgatt
     4681 tccctatcgg tctgccatta gcttatgccc gaagagcagg cataaaggaa tttattacca
     4741 tcctccctga gctgggcaaa gatcgatggt caaaattcta tgagcctgca cacacacctg
     4801 aagagatttc gattagacgg ccattctacc cagccagggc gggaaacacc cgccagcagc
     4861 acctcctgaa acaactaggc tttg
//
LOCUS       DBNGSX010000060         5677 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_142330, whole genome shotgun sequence.
ACCESSION   DBNGSX010000060 DBNGSX010000000
VERSION     DBNGSX010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5677)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5677)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5677
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_142330"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..1182
                     /locus_tag="AC16CB_01970"
     CDS             211..1182
                     /locus_tag="AC16CB_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3341262.1"
                     /translation="MMEYRYLGRTGIKVSELCLGSMQFGWTASEEESYQILSAAFEAG
                     INFIDTADVYSRWVEGNPGGVAEAIIGKWMKRNNIPRHQVIIATKVRSRIGDDPNDEG
                     LSRNHIFRAVEASLNRLGTDYIDLYQSHWYDENTPIAETMVAFDDLVRQGKVRYIGCS
                     NYPAWRLTEALWTSKHHRIVRYDAIQPHYNLVHRAEFERELADVSRAYNLGVIPYSPL
                     AAGFLTGKYRKGKVVESSRANRARRYFNDRNWSLLNQMERIGKEKGGYTISQIALAWL
                     LNDPMITSPIIGPRSLEQLEDNLGSAGLRLSQNEKKELDETSRWTDE"
     gene            complement(1188..2159)
                     /locus_tag="AC16CB_01975"
     CDS             complement(1188..2159)
                     /locus_tag="AC16CB_01975"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR3341263.1"
                     /translation="MAGETILIVEDNDVLRQGLHELLLSEGYAVLTASHGMDAIEQMK
                     TKPPDLILSDIAMPEMDGYLFFEEVRTKPEWVSIPFIFLTARRGRDEIFTGKKLGVED
                     YLVKPITRQELLTTIRSRLTRSQQLLLAQLEQAYEASLIMLANAIELRDHYTRGHVER
                     VRDHALAIAQQLGWTTNHLNYLAYGSILHDIGKIHVLESVLSKAGALDKMEWEEMKKH
                     PVIGAEMVKNISYLQSAIDIIRHHHERWDGTGYPDGLAGEDIPLSARIVAVADSFDAM
                     ITSRSYRVALTPEAAYDEILRSSGTHFDPSVVLAFQRAWGEIRNSLE"
     gene            complement(2132..4978)
                     /locus_tag="AC16CB_01980"
     CDS             complement(2132..4978)
                     /locus_tag="AC16CB_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3341264.1"
                     /translation="MISSQRWWLSLPFIGALAVFVYLIWGASLAFNFPYDGINLHSLA
                     GDFEIEPGSPAAGILREGDRILLVNDVPLIEALPLYPGKIPGDLVILTVERDGSPIEL
                     TFKLEEPSYIERLYRLVPFLVALIFWGIGVGVQAFKPAQEGSVIFFLFFQIFATALSA
                     GSSSQFAPLWTAQLLSFLLWVGGPLMVHFHFNFPQSLNIKSQRVFLAGLYSVGLVFGL
                     LYAYVGSGEARSYWANQLFIASRFFLALCFLITLWLLFYSYRNATTAGVRSKIRIVVL
                     GGAISLLPMITMGILPNALLGDTFIPQPFLFLFLGILPLTYGFAILRYRLIEIERHVN
                     RGASIILVYSILGVIYLLMSVGLDRFLPNMPFKGPVINTVLVLILATIFSPLNRFVQR
                     VVDIIFYGGWYDYRSAVSLITQGLEQITDLQSLANNTSARLLDTIRLEEVCVFLADLE
                     GDFSVISVSPNPPTGEMHRPAYAPLPRSSLSYLLNMGGAVERSVLRESLSQTVLSPQE
                     LSLLNSEQAYLWVPIIGHREVLGLMALGPKLGGDLFSGEDTDILRVVARHLAPLIENL
                     HLVTRLRLHNAMLEQRVTERTEELSASKKRLEAILGSVGEGVVVIDLDGSLLSVNAAY
                     EAQTGYNEQELFAQKLWGLYKIDKADNFYSEIQGILQAGAVWTKEMVGLRKNGSQYDV
                     QMTIAPLRDENGQIVGYVGSQRDITHQKELDRLKDMFVSDVSHELRTPTTNIGLYLEL
                     LLDAPPEKRTHYLNVLKEQSNLLIKLVEDILDLSRLAVGKEKKVEFARIKLNKLVDQV
                     VTAIRPLAEAGGLELSFNPDPNLPLVMGEPNQLSRLISNLVANAIHYTVKGDVVVRTS
                     WGDSMVCLEVHDTGIGIDPEDRPHIFERFYRGKLVRQSKIHGTGLGLAIVKEIADMHG
                     SRIDFKSDLGKGTTFYVRFPALIGEEWLVKQSLS"
     gene            complement(5144..>5677)
                     /locus_tag="AC16CB_01985"
     CDS             complement(5144..>5677)
                     /locus_tag="AC16CB_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR3341265.1"
                     /translation="TLIVMGSGQHADLTRAMLSLEQYWQQAETKSGAFFALGCRAGAR
                     LATSIDDILAGYTDYGRNFGLLIQITDDLEDVFSPHDSGTPGQKHSFQRSLPVVYALD
                     VLPSDQAARLKKCLRNATQSKEAAQDAILLVDQSGAADYLLVKIEQHKERALEALERA
                     QPRSPAGGWLAKLARDF"
BASE COUNT         1573 a         1439 c         1301 g         1364 t
ORIGIN      
        1 tacccaccaa cacctggatg aaggtgagga gctcgacatt gtgatcaagc ctgtgcccga
       61 agtattggag atgatcttgg gtaacgagat catcgatggc tctctccaac tcgggtaatt
      121 acttgccctt caaaaagggc tgattaaatc tgaatgatcc gtttccaggg cagttagcct
      181 tcttgaagta gggctgcttt tcaaaggagc atgatggaat atcgttacct gggtcgcacg
      241 ggtattaaag tatctgaact ctgcttaggg tcaatgcagt tcggttggac tgcgagtgaa
      301 gaagagtctt accaaatcct cagcgcagca tttgaggctg ggatcaattt tattgatacc
      361 gccgatgtgt attctcgctg ggtggaagga aatccaggag gggtggcaga ggccatcatc
      421 ggtaaatgga tgaagcgcaa taatatcccc cgtcaccagg tgatcatcgc taccaaggtg
      481 cgtagtcgga tcggcgatga tcccaacgac gaaggtctct cgcgcaacca cattttccgg
      541 gcagtggagg cctcgttaaa caggttaggt accgactaca tcgacctgta ccagtcacac
      601 tggtatgatg agaatacacc aattgccgaa acaatggttg ccttcgacga tttggttcgc
      661 caagggaaag tgcgttatat cggctgctcc aattaccccg cctggaggtt gacagaggct
      721 ttgtggacat caaaacacca tcgaatagtc cggtatgatg caatccaacc tcactacaat
      781 ttggtccacc gagccgaatt cgagcgagag ttagccgatg tcagccgcgc ctacaacctg
      841 ggagtgatcc cctatagccc tctggcagcc ggttttctca ccgggaagta tcgcaaaggt
      901 aaagttgtcg agagctcgcg cgcaaaccgc gcccgacggt attttaacga tcgtaactgg
      961 tccttactca accaaatgga aagaattggt aaagaaaagg gtggctatac tatctcccaa
     1021 atagccctgg catggttgct caacgacccc atgatcacca gcccgataat tggacctcgc
     1081 tccttggaac aactcgaaga taacctgggc tcggctggat tacggctttc tcagaatgag
     1141 aaaaaggaat tagatgaaac tagtcgttgg acagatgaat aagcgaacta ctccaaacta
     1201 ttccgaatct ctccccaggc acgttgaaag gccagaacca ccgacgggtc aaaatgggtg
     1261 ccactggagc gtaatatctc atcataagcc gcttccgggg tgagggcaac ccgataggaa
     1321 cgagaggtga tcattgcatc aaatgaatcc gctaccgcca caatgcgcgc cgaaagaggg
     1381 atatcctctc ctgccagacc atcaggatac cccgttccat cccaccgttc gtggtggtga
     1441 cggataatgt cgatggctga ttgcaaatag gaaatatttt tcaccatttc cgccccgatc
     1501 acggggtgtt ttttcatctc ctcccattcc atcttatcca atgccccagc tttgcttaat
     1561 acgctctcca ggacatggat ttttccaata tcatgcagaa tactgccata agctaaataa
     1621 ttcaaatggt tggtggtcca ccccagttgt tgggcaatag ctaaggcatg atcgcgcact
     1681 cgctcgacat gcccgcgggt gtagtgatcg cgcagttcaa tcgcattagc cagcataatc
     1741 aagctcgctt catacgcttg ctcaagctga gctaatagca gttgctggct gcgtgtcaaa
     1801 cgggagcgaa tcgtagtaag cagttcctgt cgagtaattg gtttgaccag gtaatcttct
     1861 acaccaagtt tcttgcctgt aaaaatttcg tcccgaccgc gcctagcggt gagaaagata
     1921 aaaggtatcg agacccattc cggtttagtg cgaacttcct caaaaaataa atatccatcc
     1981 atttccggca tggcaatatc cgaaaggatc agatctggtg gcttggtctt catctgctca
     2041 attgcatcca tgccatgcga tgcagtgagt acagcataac cttctgataa aagcagctca
     2101 tgcagaccct gtcgaagcac atcattgtct tctacgataa ggattgtttc accagccatt
     2161 cttctcctat cagtgccgga aaacgcacgt aaaatgtggt gcctttccct agatcgctct
     2221 taaaatcaat tcgactaccg tgcatatcag caatttcttt tacaattgcc aaccctaatc
     2281 cagtgccatg aattttactt tgtcgtacta atttacctcg ataaaacctc tcaaatatgt
     2341 gcggtctatc ttccggatcg ataccaatgc ccgtatcatg cacctccagg cataccatgc
     2401 tgtcacccca ggaagtgcgg acaactacat cacctttgac agtgtagtgg atggcatttg
     2461 ctaccaagtt gcttattaaa cgggaaagct ggttgggttc tcccataacc aaaggcaggt
     2521 tggggtcagg gttaaatgat aattctaagc caccagcttc tgccaacgga cgaatggctg
     2581 tgactacctg atcaaccagc ttgttcaatt taatccgggc aaattcaact ttcttttctt
     2641 ttccgacagc caacctggat agatcaagga tatcttcgac caatttaatt aacagattac
     2701 tctgttcctt aaggacgttc aaatagtggg tgcgtttctc cggtggggca tccaacaaca
     2761 attcaagata caagccaata ttggttgtgg gggtgcgtaa ctcgtgagac acatctgaaa
     2821 cgaacatatc cttcaagcgg tcaagctctt tttggtgagt gatatccctc tggctaccga
     2881 catatccaac aatttgacca ttctcatctc gaaggggggc aatcgtcatc tgaacatcat
     2941 attgactgcc atttttccta aggccaacca tctcctttgt ccataccgcg cccgcctgca
     3001 gtatgccctg tatttcactg tagaaattat ctgctttgtc aattttatag agtccccaca
     3061 gcttctgcgc gaatagctct tgctcgttat agccggtctg cgcctcgtag gcagcattaa
     3121 cgctcagcaa actaccatcc aggtcaatga ccactacacc ctcgccaacg ctaccgagaa
     3181 tggcctcaag acgttttttg gaagccgaaa gctcttcagt acgctctgtc acccgctgct
     3241 ccaacatagc attgtgaagc cggagtcgcg tgaccaggtg aaggttttct atcaatggtg
     3301 ccaaatggcg tgcaaccaca cgcaggatgt ccgtatcctc tccactaaat aaatctccac
     3361 ctaacttcgg tcccaaagcc atcaacccaa gcacttccct gtgcccaatt atcggcaccc
     3421 aaaggtaagc ttgctcgctg tttaaaagag aaagctcctg aggcgataac accgtttgac
     3481 tgagtgactc gcgtagcacg gatctttcaa ccgcgccacc catattgagc aaatatgata
     3541 ggctgcttcg aggcagtggg gcgtaggcag gtcgatgcat ttcgccagtg ggtggattag
     3601 gcgagacgga aataacagag aaatcgccct ctaggtcagc aagaaaaaca cacacctcct
     3661 ctagacgaat ggtgtccagc aagcgcgcgc tcgtgttgtt tgccagggat tgcaagtctg
     3721 taatttgctc taatccttgg gtaatcaacg aaactgcact gcgatagtca taccaaccgc
     3781 cataaaatat aatgtcaaca acacgctgta caaagcggtt tagggggctg aagattgttg
     3841 ccaggataag taccagcacg gtgttgatta ctggtccttt aaacggcata ttggggagaa
     3901 aacgatctaa cccaactgac atgagcagat agatcacacc caggatcgaa taaaccagga
     3961 tgattgatgc gccacgattg acgtgccgct caatctcgat caggcggtaa cgcagtatgg
     4021 caaagccata ggtaagcggc aatattccta ggaatagaaa gagaaatggc tgcgggataa
     4081 aggtatcacc caacagcgca ttgggcaaga tacccatcgt aatcatgggc agaaggctaa
     4141 tagctccccc taaaacgaca atacgaattt tactgcgcac cccagctgtt gtggcgttac
     4201 ggtaagaata aaacaataac catagcgtga tcaagaaaca taaagcaagg aaaaaccggc
     4261 tggcgataaa caactggttt gcccaataac tcctcgcctc tccgctgcct acataagcat
     4321 acagcaagcc aaagacaaga cccacgctat ataaaccagc aagaaataca cgctggcttt
     4381 ttatgtttag actttgggga aaattaaagt gaaaatgaac cattaagggt ccgcccaccc
     4441 aaagtaaaaa actgagcagt tgggctgtcc ataatggcgc aaactggcta ctacttccag
     4501 cactcaaagc cgtggcaaat atttggaaga ataagaagaa tataactgaa ccctcttgag
     4561 ctggcttgaa cgcctgtacc cccacgccta tgccccaaaa gatcagggca actaaaaaag
     4621 gcactaagcg gtatagacgc tcgatatagg agggttcctc caacttaaaa gtcaattcga
     4681 ttgggcttcc atccctctct acggtaagga ttacaagatc tccaggtatt ttcccgggat
     4741 aaagtggcag cgcttcgatc aacggcacat cgtttactaa tagaatacga tcgccttcgc
     4801 gcaaaatacc ggcagccgga ctgccaggct cgatctcaaa atccccagcc aggctatgca
     4861 aattaatacc atcgtagggg aaattaaacg ccaaactggc accccaaatt aaataaacaa
     4921 acactgccaa tgcgccaata aatggcaggc taagccacca acgctgggag ctgatcatgt
     4981 atcctcgccc actgagcagg gcaaaaatga ttctatcaca aggggaatag tgaaatggcg
     5041 cgatgagcgc cacctaaaca aattgttggc ttttaataat tggcctcatt cagatgcaga
     5101 ataaggggat tcttgcattt tcccgttgtt cgcaaacagc tagttagaag tctcgcgcta
     5161 gcttagctaa ccacccacct gctggagagc gaggttgagc gcgctcaaga gcctctaatg
     5221 ctctttcctt gtgttgttca atcttaacca gcaaataatc tgcagcaccg ctttggtcaa
     5281 ccaataatat tgcatcctgg gctgcttcct tgctttgagt tgcgttccgc aaacacttct
     5341 ttaagcgagc cgcctgatcg gacgggagaa catctaaagc gtatacgacc ggcaacgaac
     5401 gttgaaatga gtgcttctgg ccaggtgtgc cggaatcgtg cggagagaaa acatcttcca
     5461 gatcgtctgt aatttggatg agcagaccga agttgcgacc atagtctgta tagccagcca
     5521 aaatgtcatc aatactggtt gctaggcgtg cgccagcgcg acaacccagt gcaaagaatg
     5581 cccccgactt tgtttcggct tgttgccagt attgttccaa agacagcata gcccgggtta
     5641 aatcagcgtg ctgaccgcta cccattacga taagggt
//
LOCUS       DBNGSX010000061         2311 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_73091, whole genome shotgun sequence.
ACCESSION   DBNGSX010000061 DBNGSX010000000
VERSION     DBNGSX010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_73091"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1162
                     /gene="tgt"
                     /locus_tag="AC16CB_01990"
     CDS             68..1162
                     /gene="tgt"
                     /locus_tag="AC16CB_01990"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR3341266.1"
                     /translation="MTTSLFTFEILAQQGRARAGVFHTPHGPIPTPVFAPVGTQATVK
                     AVTPAQLDDVGANLILANTYHLYLRPGDELVSELGGLHRFMNWSAPILTDSGGYQVFS
                     LADSRQVDDDGVTFKSHIDGSTHRFTPEKSIAIQENLGADIIMAFDECAPPYDRAYNE
                     RALERTHNWAQRCQQVKRRPDQALFGIVQGGVFPDLRQRSAEFISSLDFPGNAVGGLS
                     VGETKEEMHAMLDIVLPILPVEKPRYLMGVGTPEDLIEGVLRGVDIFDCVLPTRLARH
                     NAVMTRKGRLNLVNATYARDPGPIDPECTCYTCQNYSRAYLRHLITAREILSATLLSI
                     HNLHMLIKLMQEMRQAILERRVESITTRSI"
     gene            1171..1992
                     /gene="uppP"
                     /locus_tag="AC16CB_01995"
     CDS             1171..1992
                     /gene="uppP"
                     /locus_tag="AC16CB_01995"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HIR3341267.1"
                     /translation="MTLLQSIILGIIQGLTEFLPISSSAHLVLTPYLLGWKIPAEEAF
                     VFNVLVQVATLVAVFAYYWSDVRDILTAMLVALRHNKPFNDPQARLGWYILIGTLPAG
                     LIGLNIKNTVEAAFNSPATTAFFLLITAILLFISERAGKKNKNIDQMNWIDAIWVGLF
                     QATAIFPGISRSGSTISGGMLRGLERPAGARFAFLLSIPIMLAAGFLAVMDLFGMPNL
                     SSLLPIFIPGFVVAAITGYLSIRWLLGYLVRHPLLIFSAYCVALAALSFIRGFFL"
     gene            complement(2056..>2311)
                     /locus_tag="AC16CB_02000"
     CDS             complement(2056..>2311)
                     /locus_tag="AC16CB_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341268.1"
                     /translation="RAGMLDNLPLMPSMRDLFEKGETHHRTLLSVVEDQEKVDQMVAT
                     AKRIIGDLETGHTGFLFVVPVVQAHGLGKRRWNGPSENAD"
BASE COUNT          515 a          637 c          553 g          606 t
ORIGIN      
        1 gcgattgccc tattagtagg cggctatcac aatgtgtatc gcatcaatag ggttataatc
       61 acatcttgtg actacctcgc tatttacctt cgagattcta gcccagcaag gtcgtgctcg
      121 agctggcgtt tttcatactc cccacggtcc tatccccacc ccggttttcg cccctgtagg
      181 cacccaggct actgtaaaag cggtgacacc tgcccaactt gatgacgtgg gagccaatct
      241 aattttggca aatacatatc acctgtactt gcggcctggc gatgaactag tctctgaatt
      301 gggcggacta caccgtttca tgaactggtc tgcgcccatt ctcactgact cgggtggcta
      361 tcaggtcttc tccctggctg actcacgcca ggtagatgat gatggtgtta ctttcaagag
      421 ccatatcgat ggttcgacgc accgcttcac cccagagaaa tccatagcta tccaggaaaa
      481 cttaggggca gacatcatca tggcctttga tgaatgcgca ccaccctatg accgcgcata
      541 caacgaacgg gcattagagc gcacacacaa ttgggctcag cgctgccaac aggtcaagcg
      601 ccgcccagac caggcacttt ttggcattgt tcaagggggt gtctttcccg atctacgcca
      661 gcgttcagca gagttcatat cgtctctcga ttttcccggt aacgcggttg gcggcttgtc
      721 tgtaggggag acgaaagaag aaatgcacgc catgctggat atcgttttac ccatcttgcc
      781 agttgaaaag cctcgctatt tgatgggtgt aggcaccccc gaagacttga ttgaaggtgt
      841 tctgcgtggg gtcgacatct tcgattgtgt gctaccgacc cgtctggcgc gccataacgc
      901 cgtgatgact cgcaaaggaa gacttaacct ggtaaatgca acctacgccc gcgatccggg
      961 acctatcgat ccggagtgca cctgttacac ctgccagaat tattcccgtg cctatttgcg
     1021 ccacctgata accgcccgcg aaatcctctc agcgaccctg ctctccattc acaatctcca
     1081 tatgctgatc aagctgatgc aggaaatgcg tcaagcgatc ctggaaaggc gcgtcgagtc
     1141 gatcactaca cgctcaattt gacaaaatct atgacactat tgcaatccat cattctggga
     1201 attattcaag gcctgaccga gttcctgccc atatccagtt cagctcatct cgtgcttact
     1261 ccctacttgc tgggatggaa aataccagcc gaggaggcat ttgtctttaa tgtgctggtt
     1321 caggttgcca cgttggtggc cgtttttgcc tattattgga gcgatgtgcg ggatatactg
     1381 accgccatgc tggtagcctt gcggcacaat aaacctttta acgatcctca agcccgttta
     1441 gggtggtata tcctcatcgg aacgctacct gcgggtctta tcgggttgaa tataaaaaac
     1501 acggtagaag cagcatttaa cagccctgcc actacagcct tctttctgct gatcaccgct
     1561 attttattat ttatcagcga gcgcgccggt aagaaaaata aaaacatcga tcagatgaac
     1621 tggatcgatg cgatatgggt gggtctattc caagccaccg cgatattccc cggaatctca
     1681 cgctctggtt cgaccatctc gggtggcatg ttgcgtgggc tggaacgacc agcaggggcg
     1741 cgctttgcct ttcttctctc cattccgatc atgcttgcgg ctggattcct cgccgtcatg
     1801 gacttattcg gtatgccgaa cttgagtagc ttgcttccaa ttttcatccc cggctttgtg
     1861 gttgctgcga ttacaggcta tctctccatc cgctggctgc taggctactt ggttcgccat
     1921 cccctactca ttttttcagc ttactgcgta gcactggcag cgctaagttt tatccggggt
     1981 ttctttctat aagtgtttgc tagataactc atagcttgaa aatctttgta accgggcttc
     2041 ctattaaaga tccgattaat cggcgttctc actcggccca ttccatcggc gcttaccaag
     2101 cccgtgggcc tgaaccaccg gcacaacaaa gaggaagccg gtgtgaccag tttccagatc
     2161 gccaatgatg cgtttggcgg tggcgaccat ctggtcgacc ttctcctggt cttcaacaac
     2221 agaaagaagc gtgcgatgat gggtctctcc tttctcgaaa agatcccgca tactgggcat
     2281 caaaggcaaa ttgtcaagca ttcccgcgcg g
//
LOCUS       DBNGSX010000062        10819 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1458468, whole genome shotgun sequence.
ACCESSION   DBNGSX010000062 DBNGSX010000000
VERSION     DBNGSX010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10819)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10819)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10819
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1458468"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="AC16CB_02005"
     CDS             <1..914
                     /locus_tag="AC16CB_02005"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3341269.1"
                     /translation="HGSEICWKKFISAGKFYEVDTLILGGDMTGKAIVPIIAQGNDKY
                     KVTLLEQETILDGREEVEKVVKTIQNRGYYPYVTDPEEVAEISSTPGRSDELFLSQAL
                     ETVERWMAYAEEKLSGTGIRCYVCPGNDDMMEIDAVIAASKSVQLAEGRVVRLDDHHE
                     MASTGWSNPTPWDTHREEPDEALLERIEAVISQVQDIPNAVFNLHPPPYGSGLDEAPE
                     LTEDLRPAYAGRSLIPVGSRAVLKVIEKYQPLVSLHGHIHEGKGTRKLRRTLCINPGS
                     MYEQGILHGVVMELKPKGLGNYLMVTG"
     gene            1090..2949
                     /locus_tag="AC16CB_02010"
     CDS             1090..2949
                     /locus_tag="AC16CB_02010"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIR3341270.1"
                     /translation="MSEVTLFVRKASGLVRSWSIFDAFIYATFSINLITLGLFIFSYC
                     YYFEGNLATAVVVGGAFTIFEVIVYASLISAMPRAGGDYVWQSRILGRGWGFILAVTG
                     WWFILWLWVPLYGQMLNYEFFTPILGVLGAKDAALWFTNTPEGLLTGMLLVCALVFVY
                     IGIGMKWYARVQKFCFWGGMVGLLLVFILLLFGNNATFVNNLNTMMPKLFGTEPGVDL
                     YQATLDAGAEAGSVVLPLGNIAFLASFAVIPMIVFFNLWPNWGSTLYGEVRGASDYKR
                     NFWGMAAAVVVTAALALILFALISKTIGWNFYNSANGAFWNYTWGYTDQPPPLPIWPY
                     PVLFAAMLTTSPVLQFIVVLLVSLWWFGWSGTVFLSSTRVIFAAAFDRMLPEWASAVE
                     PRTRTPVNALLLMVIPSVVISILYAYDIFSLQTIVLDATLVIAVTFLGTTVAAILLPW
                     RQKEVFDGSPIAKYQVPSWLSWIVTLLYALAAIYLIRRTYTVGLEVFGGFADLGALTR
                     LMVWLIAILSVVNAALLVWIFYFAIRKLLSGETFPLITVAGLIFFGFLDWLLVEWFWD
                     PGYLYGIGWQNDISMLFMVAMYGLAAAIFYGFNAYRRRQGIDVDKVYKEIPVE"
     gene            3230..4390
                     /locus_tag="AC16CB_02015"
     CDS             3230..4390
                     /locus_tag="AC16CB_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3341271.1"
                     /translation="MPQKTSISRRDFLKMSALTGGAAFLAACGGGPAAPSGANLPDLT
                     SGNSIPLEELIAAAKTEGELTTIALPHDWANYGEIIETFKTKYGLKVNELDPNAGSAD
                     ELEAIRANKESKGPQAPDVIDVGVGHTETAMKDGLVAKYKVSTWDSIEMKDPDGYWWA
                     EYYGVLVFEVSKPAIENSPQDWEDLLKPEYKGKIAMAGDVLKSNQSVQTVLASGLSRA
                     GGDITKAAEAGLQFWKEVNDAGNFIPVIADQGTIAQGETPIVMEWDYLALANRDSLAG
                     NPDLEIVVPKTGVLAGPYAGGISAYAPHPYAARLWWEFVMSDEGQNLYLKGYAHPIRF
                     NDMAKRGVIPQEMLDKLPPAAAYEKAFFPTIQQLTETKTYITENWRKVVYGE"
     gene            4648..5553
                     /locus_tag="AC16CB_02020"
     CDS             4648..5553
                     /locus_tag="AC16CB_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006469433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3341272.1"
                     /translation="MSVARGQPSSGSKAGGGPSKRFSWDWVGLAPFFAFAIMFLFLPS
                     VSIFIRSFEAPGGGFTISNLVALVTQRDLINAYKTSLSISLITATGGAIFGFLLAYAI
                     VLGGLPPALRTFMTTFSGVASNFAGVPLAFAFIATMGNAGLVTRWLESALGLDIHARG
                     FTIYSIWGLSIVYMYFQFPLMVLIMAPALDGLRREWKEACQNLGGTNWHYWRHVAFPI
                     LLPSLLGTTILLFGNAFGAYATAYAFSGSFINLVTIVIGAQIQGDVLYNPGLGNALAL
                     GMIIIMSLSMSAYIYLQRLSARWVR"
     gene            5610..6416
                     /locus_tag="AC16CB_02025"
     CDS             5610..6416
                     /locus_tag="AC16CB_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3341273.1"
                     /translation="MKKSSSVWAWFIFLIGIIYFFLPLFATFEFSMRMIKDTYSFEAY
                     RVAFTDPRFFLSFGYSLLWGILTIFVSLLLVVPTAYWVHWRMQRARPFVEFVTLMPFV
                     VPAVVLVFGMSSLYGGALKIGGFPILVLTPTPILLIAGYVVLSFPYMYRAVDTGLRAI
                     DVRTLTEAALSLGAGWRVILTQVIFPNLRVAILSGVFLTFAIVMGEFTFASLLVWPAF
                     GPYMEEIGAMRAYTPQALSVVSFILTWASIGLINWVGRGAPGSQQVAGAH"
     gene            6434..7495
                     /locus_tag="AC16CB_02030"
     CDS             6434..7495
                     /locus_tag="AC16CB_02030"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008124572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3341274.1"
                     /translation="MAFLELHNLSKSFGTTEAVSDFNLDIEKGELVSFLGPSGCGKTT
                     TLRMVAGFETPTTGHIKIDGMDITNNPPNQRDVGMVFQAYALFPNMTVANNIGFGLKI
                     AKKPPEEIKSRVEEMLSLIHMNGFGDRYPYQLSGGQQQRVALTRALALSPQVLLLDEP
                     LSALDAKIRVSLRSEIRAIQQQLGITAIYVTHDQEEALSLSDRVVVMNAGRMEQVGPP
                     FEIYNYPTTEFVAQFVGTLNAVSAEVVDPASQTLRLEGQLLQTEENLDGRRSGERVMI
                     AIRPERLSFAAEEKKANVIDCSIENITFLGSIVRIQVRLGNVSFSMDTFNNPYLELPK
                     IGDKTQITCSRKAVLILKQ"
     gene            7492..8301
                     /locus_tag="AC16CB_02035"
     CDS             7492..8301
                     /locus_tag="AC16CB_02035"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR3341275.1"
                     /translation="MNKVILIISDGLRYDTAVARMGYLGHLVEAKHASLYKVNGELPS
                     MSRPMYETIHTGLLTNEHGIVSNAVVRRSTKPNLFELAAKAGKTTAAAAYYWFSELYN
                     RAPFDPIEDREVDDKALLIQHGRFYTQDDYPDIDLFNTAALLVRRFQPDYLLIHPSGM
                     DYTGETYGSDSSQYRNHAIRQDVWLSKHIVEWLERDYSILVTGDHGISADRTHGGTTP
                     DMREVPLYMLGTGIQGRGDTGEVLSQLQVAPTICRLLDIPIPETMRATPIL"
     gene            8478..9461
                     /locus_tag="AC16CB_02040"
     CDS             8478..9461
                     /locus_tag="AC16CB_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005315 - phosphate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HIR3341276.1"
                     /translation="MNLLIIIFVAIALLFDFLNGFHDSANVVATAIASRAMSPRFALG
                     MTALANFAGPFLFGVAVAKTIGNEVVGSEAVTVPVALAALISAIIWNVLTWLLGIPSS
                     SSHALIGGFVGAAVTGFGMSIVHLEGMVKVLIGLFISPLIGLVLGWFVMRQSLFVGRH
                     SPPSINLFFRRAQWLTALTLGLSHGTNDAQKTMGIIALGLVAFGVLPEFRVPFWVIAA
                     SAGAIALGTALGGWRLIRTMGAGFYRIRAIHALSSQVASTIVILGAAILGAPVSTTQV
                     ISSTIVGAGAAHRVQMVRWSVATQIVVAWILTIPATGILGAVFFLLLRPIL"
     gene            9478..10131
                     /locus_tag="AC16CB_02045"
     CDS             9478..10131
                     /locus_tag="AC16CB_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013982.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HIR3341277.1"
                     /translation="MKKSPWWKKFNFQSVFKRRPNRFIELLTQQAQITEEGLTALVAY
                     FEKPTKRRADVVSKLEGNADEIRRILIDELNHTFVTPFDREDIHALSRAIDDMLDYAY
                     TTVVEMSILEVEPTSHMSEIGALLAKAASEVHLGVQRLADHPGVAQNHAVRAKQLENQ
                     VEHLYREALADLFMQPKDAEHIVEMLKLRETYRHLSNAADRGDDAANVLSDIVVKMG"
     gene            complement(10134..>10819)
                     /locus_tag="AC16CB_02050"
     CDS             complement(10134..>10819)
                     /locus_tag="AC16CB_02050"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3341278.1"
                     /translation="VAGSGFSAAVYFLGLARAFESSDFTIVYPVARSLPVIFVAFIDV
                     LRGRHLTSFGWVGILLVVAGCVLVPTKSLKDIKISNYFNRTSLWMLLAAMGTVGYTLL
                     DKIAAETLQQGPDTAARYGYFYFTISFLPYLFFMKLFKPKTARPPTNDWKLAIPAAVL
                     GFSAYWLILWAYQLTPNASYIVAFRQFSIVIGAILAFIIYKEGGVKMRLSGAMLITSG
                     LVLIAGWGR"
BASE COUNT         2310 a         3113 c         2930 g         2466 t
ORIGIN      
        1 ttcatggttc ggaaatctgc tggaaaaagt ttatcagcgc cggaaaattc tacgaggtgg
       61 acaccctgat cctgggtgga gatatgaccg gcaaggctat cgtgcccatc atcgcccagg
      121 gcaatgataa atataaggtc accctcttgg aacaagagac catcctagac gggcgcgaag
      181 aagtggagaa ggtggtcaag accatccaga accgagggta ctatccttac gtgacggacc
      241 cggaagaagt ggcagaaata tcgtctaccc caggcaggtc ggatgagctc ttcctatcgc
      301 aagcacttga gaccgtggag cgctggatgg cctacgccga ggagaaactg tcaggcaccg
      361 gaatccgctg ctacgtctgc ccgggcaatg acgacatgat ggagattgat gcagtcatcg
      421 ccgcctccaa gagcgtgcaa ttggccgagg gccgggtggt cagattagac gaccatcacg
      481 agatggccag cactggttgg agcaacccca ccccctggga cacccaccgc gaagaacccg
      541 acgaagccct cctcgaacgt atcgaggcgg tcatatccca ggtgcaggat attcccaacg
      601 ccgtgttcaa cctgcacccg ccgccatacg gcagcgggct ggatgaagcg cctgagctga
      661 ccgaggatct gcgccctgcc tatgccggcc gctctttgat ccccgtgggc agccgcgccg
      721 tcctcaaagt gatcgaaaaa taccagcctc tagtgagcct gcacgggcat atccatgaag
      781 gcaagggcac gcgcaagtta cgcaggacgc tgtgcatcaa ccctggcagc atgtacgagc
      841 aaggcatctt gcatggtgtg gttatggagc tgaaacctaa aggcttgggc aattacctga
      901 tggtgacagg ttgagctcct caccccttcc cggttcccct acccttattc caagggagat
      961 aggggaaaag gttggggtga gggaattaga ggaaggaggt ggacctgagt agattggaac
     1021 acataaacaa aagtacccgg ttttcaatcc gttcatcatt agatattcca aaaatggagg
     1081 agtactgaca tgagtgaggt aacacttttt gttcgcaaag caagcggcct ggtgcggtcc
     1141 tggtcgatct ttgacgcgtt tatctacgcc accttctcga tcaatctgat tacgctaggt
     1201 ctgtttatct tctcctattg ctactatttt gagggcaacc tggcgaccgc ggtggtcgtc
     1261 ggtggggctt tcaccatctt cgaggtgatc gtctacgcca gcctgatctc tgccatgccg
     1321 cgcgccggtg gcgattacgt ctggcaatcg cgcatcttgg ggcgaggctg gggcttcatc
     1381 ctggccgtca ccgggtggtg gttcatcttg tggctgtggg tgccgctcta cgggcagatg
     1441 ctcaactacg agttcttcac ccccatcttg ggcgttctgg gcgccaagga tgccgccttg
     1501 tggttcacca atacccctga aggtctgctc accggcatgc tactggtgtg cgccctggtc
     1561 ttcgtctaca tcggaattgg catgaagtgg tatgcccgcg tgcagaaatt ctgtttctgg
     1621 ggtggcatgg tcggcctgct gctagtcttc atcctgctac tcttcggaaa caacgccacc
     1681 ttcgtcaaca atctcaacac catgatgccc aaattgttcg gcaccgaacc aggtgtcgat
     1741 ttgtatcaag ccacattgga cgcgggtgcg gaagcgggct cggttgtctt gcctctaggc
     1801 aatatcgcct tcctggctag cttcgccgtg atccccatga tcgtgttctt caacctgtgg
     1861 ccgaactggg gctccacgct gtacggcgag gtgcgcggcg cttcggacta caaacgcaac
     1921 ttctggggca tggctgccgc cgtcgtcgtg actgctgccc tggcgttaat cttattcgcc
     1981 ctgatcagca agactatcgg ttggaacttc tacaacagcg ccaatggcgc tttctggaac
     2041 tacacctggg gctacaccga tcaaccaccg ccacttccta tctggcccta cccggtgctc
     2101 ttcgccgcta tgctgaccac cagcccagtg ctgcagttca tcgtggtgct gctggtgagc
     2161 ctgtggtggt tcggctggtc gggtacggtc ttcctgagct ctacgcgcgt catcttcgcc
     2221 gcagctttcg atcgcatgct gccagagtgg gcctcggctg tcgagccgcg cacgcgcacg
     2281 ccagtgaacg ccctgctcct gatggtcatc ccatcggtgg ttatctcgat cttgtacgcc
     2341 tatgatatct tcagcctgca aaccattgtg ttggatgcca ccctggtcat cgccgtgacc
     2401 ttcctgggca ccaccgtggc agccatcctc ctgccctggc ggcagaaaga ggtcttcgat
     2461 gggtcaccga tcgccaagta ccaggtgcca agctggctga gctggattgt aacgctgctg
     2521 tatgcgcttg ccgcaatcta ccttatccgc cgcacctata cagtaggatt ggaggtattt
     2581 ggcgggtttg ccgatctggg cgccttgacc aggctgatgg tatggctgat cgccatcttg
     2641 agtgtggtca acgcagcgct gctggtgtgg attttctact ttgccatccg taagctactg
     2701 agcggcgaga cgttcccgct cattacggtt gccggtttga tctttttcgg cttcctggac
     2761 tggctgctgg tcgagtggtt ctgggacccc ggctacctgt atggcatcgg ttggcagaat
     2821 gacatctcga tgctctttat ggtggcgatg tacggtctgg ccgcggccat cttctacggc
     2881 ttcaacgcct accgccgccg ccagggcatt gacgtggaca aggtctataa agaaattccg
     2941 gttgaatagg tctccatgtg cgacgcacca ataaggtgcg tcgcacatta cttccgatta
     3001 ttaaaaactg ctttgacttt gctggttaat gatgctacaa tttacccctg cgcgcttttt
     3061 accatgcgcg cttcccatta cagctacatg tcgcttccat ttgtgtaatg tcgggggcga
     3121 atattagcca tacaggaggt gaaccgcgca aacacttctg agttaaaatg cactttcaac
     3181 caccaatctt tattgaatta ctcatactaa acaaacttcg gaggacaaga tgccacaaaa
     3241 aacatccatc agccgccgcg atttcctgaa aatgagcgct ctcaccggcg gagccgcctt
     3301 cctagcggct tgcggaggtg gtcctgctgc tcctagtggg gccaatctgc ccgacctgac
     3361 ttctggtaac tccatcccct tggaggagct gattgccgcg gcgaagacag aaggcgaact
     3421 gaccaccatc gcccttccgc acgactgggc aaactacggt gagatcatcg agacctttaa
     3481 aaccaagtat ggattgaaag tcaatgaatt ggacccgaac gccggctcag ctgacgagct
     3541 ggaagccatc cgggccaata aagagagcaa aggcccgcaa gcccccgacg tgatcgacgt
     3601 cggcgttggc cataccgaaa cggccatgaa ggacgggctg gtggctaagt acaaagtgtc
     3661 tacctgggat agcatcgaga tgaaggaccc ggatggctac tggtgggctg agtattacgg
     3721 tgtgctggtt tttgaggtgt ccaagccagc tatcgagaac agcccccagg actgggaaga
     3781 tctgctcaag ccagagtaca aaggcaagat agccatggct ggcgatgtgc tcaagtccaa
     3841 ccagtctgtg caaaccgtgc tggcgtctgg cttgtctcgc gccggtggtg atatcaccaa
     3901 ggcggcagaa gcaggtctgc agttctggaa ggaagtcaac gacgctggca acttcatccc
     3961 cgtcattgca gaccagggca cgattgccca gggagagacg ccgattgtga tggaatggga
     4021 ctacctggcc ctggccaacc gagactccct ggcgggcaac cctgatctgg agattgtagt
     4081 tcccaagacg ggtgtgctcg ccggcccgta cgccggcggc atcagcgcct acgccccgca
     4141 cccctacgct gcccgcctgt ggtgggagtt cgtcatgagc gacgaaggcc agaacctgta
     4201 cctgaagggt tacgcccacc ccatccgctt caacgatatg gccaagcgcg gcgtgatccc
     4261 gcaagagatg ctggacaagc tgccgccagc cgcagcttac gaaaaagcgt tcttcccgac
     4321 catccaacaa ctgaccgaga ccaagactta tatcactgag aattggcgca aggtcgttta
     4381 tggcgagtag aaatttcggt cgttgatcaa cccgcagttc tactgcgctt gaatacaaaa
     4441 tgttcattgc gagactgcct gcttgacctg tctccagcgg gcagtctcca aagaaactgt
     4501 cactgcgagc ctgcgagcct gccgttttca cctaacctgt aggtctggac aggtcggcag
     4561 gcgaagcagt ctcacccgtc gtcaagtcga gattgcttca gtcgtccgaa tggctccttc
     4621 gcaatgactt gtcaggaggt gctgaccatg agtgtagccc gaggacaacc atcatccggc
     4681 tccaaagccg ggggaggtcc atccaaacgt ttttcctggg attgggtggg gcttgccccg
     4741 ttttttgctt ttgccatcat gttcttgttt cttccgtcgg tttcgatctt catccgcagc
     4801 ttcgaagcgc ctggtggtgg cttcactatc agtaatttgg ttgccttggt gacccaaagg
     4861 gatctcatca acgcctacaa aaccagcttg agcatcagcc tgatcacagc cacggggggt
     4921 gctatcttcg gcttcctgtt ggcttatgcg attgttctag gagggctgcc gccggctcta
     4981 cgcactttca tgaccacctt ttcgggtgta gcctccaatt ttgccggcgt gccgctagca
     5041 tttgccttta ttgccaccat gggcaatgcc gggctggtca cccgctggct tgagtctgct
     5101 ctgggactgg acattcacgc ccgaggcttc accatctact cgatatgggg cttaagcatc
     5161 gtctatatgt acttccagtt cccactgatg gtgctgatca tggcccccgc cctggatggc
     5221 ctgcggcgcg agtggaaaga agcttgccaa aacctgggcg gcacaaactg gcactactgg
     5281 cgccatgtag ccttccccat cctgctgccg tccctactgg gcaccaccat tctgttattc
     5341 ggcaatgcct ttggtgccta cgccactgct tacgccttct cgggcagctt catcaacctg
     5401 gtgaccatcg tgatcggcgc ccaaatccag ggagatgtgc tgtataaccc cggcttgggc
     5461 aatgcactgg cgctgggcat gatcattatc atgtcgctgt caatgtctgc atatatctat
     5521 ctgcagcgcc tgtcagcgcg atgggtgcga tgagagatga ttctaaatag acgtaaagat
     5581 cacgccgatc caaggcgaaa ggaacgacta tgaaaaagag ttcaagcgtc tgggcttggt
     5641 tcattttctt gatcggtatc atctacttct tcttgccgct cttcgctacc ttcgagttca
     5701 gcatgcggat gattaaagac acctattctt ttgaagccta ccgggtagct tttacagacc
     5761 cgagattttt cctcagtttc ggctactctc tcctgtgggg gatcctgacg attttcgtca
     5821 gcctattgct ggtcgtaccg actgcttatt gggtgcactg gcgcatgcaa cgcgcccgac
     5881 cgtttgtcga gttcgtcacc ctgatgcctt tcgtggtgcc ggccgtagtc ctcgtttttg
     5941 gcatgagcag cctgtacggt ggcgcgctga aaattggcgg cttccccatc ctggtgctga
     6001 cccccacccc catcctactg atcgccgggt atgtggtttt atctttccct tatatgtatc
     6061 gcgcggtaga taccggcttg cgcgccattg acgtgcgcac cctcaccgag gctgccttga
     6121 gcctgggggc tggctggcgg gtcatcctga cgcaagtcat cttccccaac ctgcgcgtgg
     6181 ccatcctgag cggcgttttt ctgaccttcg ccatcgtgat gggcgagttc accttcgcct
     6241 cgctgcttgt ctggcctgct tttggccctt atatggaaga gatcggcgcc atgcgagcct
     6301 acacgccgca agcgctgtca gtcgtcagct ttattctcac ctgggcatcc atcggcctga
     6361 tcaactgggt tgggcgcggc gcccccggct cacagcaagt cgccggagca cactaataag
     6421 aagaggtcat aacatggcat ttctggaatt gcacaacctg agcaaatcct tcggcacaac
     6481 cgaggcggtc tctgatttca atctggacat cgaaaaaggc gagctggtct ctttcttggg
     6541 tcccagcggc tgcggaaaaa ccaccacact gcgcatggtg gctggctttg agactcccac
     6601 caccggtcat attaagattg atggcatgga catcaccaac aaccccccca accagcgcga
     6661 tgtcggcatg gtatttcagg cctacgccct atttccaaac atgaccgtag ccaacaacat
     6721 cggctttggc ctaaaaatcg ctaagaaacc gccggaggag attaagtccc gggtagaaga
     6781 gatgctctct ttgatccata tgaacggttt cggtgaccgc tacccctacc agctctccgg
     6841 cgggcagcag cagcgcgtcg ccctgacgcg tgccctggca ttaagtcctc aggtgctgtt
     6901 gctggatgag ccgctttccg ccttagatgc caagatccgc gtctcgctgc gctccgagat
     6961 ccgcgccatc cagcagcaac tgggcatcac cgccatttac gtgacccacg accaggaaga
     7021 ggctctatcg ctatccgacc gggtggtggt gatgaacgcg gggcgcatgg aacaggtggg
     7081 gcctcccttt gagatctaca actaccccac cactgaattc gtcgcccaat tcgtgggaac
     7141 tctgaacgct gtcagcgctg aggtggttga cccagcttct cagacattaa ggctcgaggg
     7201 gcaactgtta caaaccgagg aaaacttgga tggacggcgt tctggtgagc gggtgatgat
     7261 cgccatccgc cccgagcgcc tgagttttgc cgccgaagag aagaaggcca acgtcattga
     7321 ttgcagcatc gagaacatta cctttttggg ctctatcgta cgcatccagg tgcgcttggg
     7381 gaacgtcagc ttctctatgg acacattcaa caacccgtac ctggagctgc ccaagatcgg
     7441 ggataaaaca cagatcacct gctcgcgcaa ggccgttctg atcctaaagc agtgaacaaa
     7501 gttatcctga tcatttccga tgggctgcga tatgatacgg ccgtcgccag gatgggctat
     7561 ttaggacacc tggtggaagc caagcacgcc agcctgtata aagtaaatgg cgagctgccc
     7621 agcatgtccc gtcccatgta tgagaccatc cacactggcc tgctcaccaa tgagcatggc
     7681 atcgtctcca atgctgtagt gcgccgctcg acgaaaccta acctctttga gctggcagca
     7741 aaggccggga agaccactgc ggcagccgcc tactactggt tctccgaact gtataaccgc
     7801 gccccttttg accccatcga agaccgcgag gtagacgaca aggcgctgct cattcagcac
     7861 gggcgcttct atacccagga cgattaccca gatatcgact tattcaatac cgcggcactg
     7921 ctggtgcgcc gcttccagcc agattacctg ctgatccacc ctagtggcat ggattatact
     7981 ggtgagactt atggatccga ttccagtcag taccgcaatc acgccatccg ccaagacgtg
     8041 tggctgtcaa aacacatcgt ggaatggctg gaacgtgatt actcgatctt agttaccggc
     8101 gatcatggga tcagcgctga ccgcacccat ggcgggacca cgccagacat gcgcgaggtg
     8161 ccgctttaca tgttgggtac gggtatccaa ggcaggggcg acacagggga agtgctctcc
     8221 cagttgcagg ttgcccccac gatatgccgg ttgctggata ttcccatccc agaaaccatg
     8281 agggccacgc caatcctcta agccgtgccc gcatacatgt aggaaaagtg tcattattga
     8341 cgttctcagc caccaagatg acctcttcac aatatctgtg aaggcctaga tggcacaaag
     8401 aacgctacct ggtaccagaa gacatcaatt ctcaaacaga tcacggggtg gctgtccaag
     8461 gtaaggagtc tgtatcgttg aaccttctca tcattatctt cgtagccata gctttgctat
     8521 ttgattttct gaatggtttc catgacagtg ccaatgtggt cgccacggcc attgcctcac
     8581 gagccatgtc gccgcgcttc gccttgggca tgaccgccct ggcaaacttt gctgggccgt
     8641 tccttttcgg cgtggcggta gccaaaacca tcggcaatga agtcgtcggc tccgaggcgg
     8701 tcactgtgcc cgtggcgctc gccgccctga tctctgccat catctggaac gtgctcacct
     8761 ggttgctggg catcccctcc agttcctcgc atgcgctgat cggcggcttc gtaggcgcgg
     8821 cagttaccgg ctttggcatg agtatcgtgc atctggaagg catggttaaa gtcttaatcg
     8881 gtttatttat ctccccgctg attggcctgg tcctcggctg gtttgtcatg cgccaatccc
     8941 tcttcgttgg gagacattca cccccaagca tcaatttatt tttccggcgc gcccagtggc
     9001 tcacagcctt gacgctgggg ctcagtcacg gcaccaacga tgcccagaag accatgggca
     9061 tcatcgcttt ggggttggtg gccttcggcg tcttgcccga gtttcgtgtc ccattttggg
     9121 tgatcgcagc cagcgcgggc gccattgcct tggggacagc cttaggtggc tggcggctga
     9181 tccgcaccat gggtgccggc ttctaccgca ttcgcgccat tcacgcgctg tcttcacagg
     9241 tcgccagcac aatcgttatc ctgggtgcgg cgatcttggg tgcgccggtg agcaccaccc
     9301 aggtgatcag ctcaaccatc gtaggtgcag gcgccgctca ccgggtgcaa atggtgcgct
     9361 ggagcgttgc cacccagatt gtcgtggcct ggattctgac catcccggca actggaattt
     9421 taggcgcagt tttcttctta ttactgcgtc caatcttgtg aaaggtataa tccttgtatg
     9481 aaaaagagtc catggtggaa aaaatttaat ttccaatcag tattcaagcg gcgacctaat
     9541 cgcttcattg agctactcac ccagcaagcg caaatcactg aggaaggact gactgcactg
     9601 gtggcatatt ttgaaaagcc caccaaacgc cgtgccgacg tggtcagcaa gctggagggc
     9661 aacgcggatg agatacgtcg tattctgatt gatgagctga accatacctt tgtcacgccc
     9721 ttcgaccgcg aggacattca cgccctgtca agagccatcg acgatatgct ggattacgcc
     9781 tataccacag tggtggaaat gtcgatcctg gaggttgagc ccacctccca catgagcgaa
     9841 attggcgcgt tactggcaaa ggctgccagc gaagtgcacc tgggggtgca gcgcttggct
     9901 gatcaccctg gcgtcgccca aaatcacgcc gtgcgcgcca agcagctcga gaatcaggtg
     9961 gaacacctgt accgtgaagc cctggcagat ctttttatgc agccgaaaga cgctgaacac
    10021 attgtcgaga tgctcaagct gcgcgagacc taccgccacc tgagcaacgc tgctgaccgc
    10081 ggcgacgacg cagccaatgt actgtcagat attgtggtga agatggggta gtattatctc
    10141 ccccaccctg cgatcaacac cagccctgag gtgatcagca tggccccgct taagcgcatc
    10201 ttcactcccc cctctttgta aataataaat gcaaggatag caccaatcac gatgctgaac
    10261 tggcgaaaag ccacaatata gctggcattg ggggtgagct ggtaggccca taagatcagc
    10321 caatatgccg aaaagcccag caccgcagcg ggaatagcca atttccagtc gttcgttggg
    10381 ggtcttgccg tcttgggctt aaacagtttc atgaagaata aatacggcaa gaacgagatg
    10441 gtgaagtaaa aatagccata ccgcgccgca gtatcaggac cctgctgcaa ggtttcggcc
    10501 gcgatcttgt ctaacagggt gtaaccaaca gtccccatag cagccagcag catccacagg
    10561 ctggtgcggt tgaaataatt gctaattttt atatccttca gcgacttggt tggcacaagc
    10621 acacagcccg caaccaccag gagtatccca acccagccaa aagatgtcag gtgccggccg
    10681 cgcaacacat cgataaaggc cacaaagatc accggcagcg aacgcgccac agggtacacg
    10741 atggtaaaat ccgacgattc gaaggcccgc gccagaccca aaaaatacac cgccgcgctg
    10801 aatcccgaac cggccacac
//
LOCUS       DBNGSX010000063         4521 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1666217, whole genome shotgun sequence.
ACCESSION   DBNGSX010000063 DBNGSX010000000
VERSION     DBNGSX010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1666217"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="AC16CB_02055"
     CDS             complement(<1..386)
                     /locus_tag="AC16CB_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341279.1"
                     /translation="MKRPPLYTLLRLLLILSPLHLGSAAAPPPPTDTPEARTMSIENA
                     IQQEITNQQDSSLIYLLFETQIGQVSISENGDWAFAQLIPLDAKTGAVVPAEPGLALA
                     QNIEGSWHIFLPNDPQWSGTLNAVPDS"
     gene            576..1328
                     /locus_tag="AC16CB_02060"
     CDS             576..1328
                     /locus_tag="AC16CB_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341280.1"
                     /translation="MTVNFTRAAVALLYFRGYSQVDGILLTWETEVEPDIQGFYIMRS
                     DIENGNYERVSQFIPGLGSDTTGDYYTYIDSNVVGGVAYWYQLEMVDVNNLVTFSEKI
                     RVFAGIYSSTPTVTATALLKSATPTFSQLALTATQGVSATSQVTPSPAETSTSIQETT
                     SGFTEVVTATAVVSATATLIPLPEITLQFPTAGANPESANAGTNTISESTSAERDQQL
                     LVRLGRATILVLIVLVWAVLAGWFYISARRME"
     gene            complement(1368..2510)
                     /gene="tal"
                     /locus_tag="AC16CB_02065"
     CDS             complement(1368..2510)
                     /gene="tal"
                     /locus_tag="AC16CB_02065"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HIR3341281.1"
                     /translation="MTKLHELAALGQAIWLDYIRRAFITSGEMQALIDLGVRGMTSNP
                     TIFEKAILSGQGSKPDQLGEYDSDLERLSKAGKTPLAVYEALAIEDIQAAADLLRSVY
                     DDTEGGDGYVSLEVNPTLASQTMETIEEAHRLWLQVDRPNLMIKIPATVEGLPAITAS
                     IASGINVNVTLIFSLTRYHQVVDAYLKGLEQRLAAGKSINHVASVASFFVSRVDSKVD
                     KHLDSILLENGPHAELAASLKGKAAVANAKLAYSLFQELFGSQRFIALQSQGACVQRP
                     LWASTSTKNPAYSDILYVQELIGPHAVNTVPQNTLEAFLDHGEVRQTISEDIDEVRLV
                     LEKLENLGISMEQVTKELEDEGVEAFAKSFTSLLESIESRQNKLRR"
     gene            3165..3944
                     /locus_tag="AC16CB_02070"
     CDS             3165..3944
                     /locus_tag="AC16CB_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341282.1"
                     /translation="MTPKSLKRVADQALFSGVFLPYWTFDTTATASWRAEVGHTETER
                     YFQDGEWKTRTVIRWRWESGKVNEKFDDLLVPGTNRLSSLLLNKIKAFQMSQLRPYEA
                     GFLAGFQALSYDRPLDNSWEIARQQMREHTRLACRKQASTPRIRNFSMKLDFGNESWR
                     YILLPIYLAGYRYANQNFQVIINGQSGQIAGQRPVNWKKVWLVVGALLTPGLLLALIG
                     AITLLLGGVGALIGGVGFVLLLIGVVISVVILRQATSMDDI"
     gene            4077..>4521
                     /locus_tag="AC16CB_02075"
     CDS             4077..>4521
                     /locus_tag="AC16CB_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341283.1"
                     /translation="MTKPCPHCFHEALALIEQDSSQFPDLKPPELVMPFEIQAEAVLR
                     GIERFAEGIRFAPADLKADQLIRRLKRVYVPAWLVDVDVQATWELEAGFDYQVVSHQD
                     RYNEIGGGWTSQQVIEGRIRWEPRVGRLTRTYQNISAPAIEDDREI"
BASE COUNT         1144 a         1003 c         1134 g         1240 t
ORIGIN      
        1 gaatcaggta cagcattcag ggtgcctgac cattgtgggt catttgggag gaaaatatgc
       61 caactcccct caatgttctg ggctagggct aatccaggtt cagccggtac tacggcacct
      121 gtctttgcgt cgaggggaat tagctgggcg aaagcccagt cgccattttc tgagattgag
      181 acttgtccaa tctgggtttc aaagagcaga taaatcaaag aagaatcttg ctggttggtg
      241 atttcttgtt gtattgcatt ctcgatggac atggtgcgcg cttcgggggt atcagtgggt
      301 ggtggaggag cagctgcact tccaagatgc aacggcgata gaatcagtaa taatctcagt
      361 agcgtataca aaggtgggcg cttcatgcat taatgatact tcacatagat cgttgcatga
      421 agagtgtgcc gttacagact tgttaggcaa gataatttcg tttcattggt ataattccca
      481 cacttctctt tcatatgagc atctcatccc tccgttcgaa agaacgctcc cgaatctttc
      541 tcattggcct catattattg ctgacgataa cattcttgac tgtcaatttt acgcgtgctg
      601 cggtagcgtt gctctatttc cgcggttata gccaggttga cggcatcctt cttacctggg
      661 aaaccgaggt tgaacctgat attcagggtt tttacatcat gcgcagtgac atcgagaatg
      721 gaaactacga gcgggttagc cagtttattc ctgggttggg tagcgataca acgggcgatt
      781 actataccta cattgactct aatgttgtgg gaggagtggc gtactggtat caattagaga
      841 tggtcgatgt aaataatcta gttacatttt ctgaaaagat acgggttttc gcaggtatat
      901 atagttcaac accgactgta acggcaacgg cacttttaaa gagtgcaacg ccaacattta
      961 gtcagcttgc gctcacagct acccaggggg tatcagcaac ctcccaggtg accccctcgc
     1021 cggctgaaac atcgacgagt attcaggaaa caacatcggg cttcactgag gttgtgactg
     1081 ctaccgcggt cgtatctgct acagcgacac ttatcccctt gcctgaaata acattacaat
     1141 ttcctactgc aggcgcaaat ccagagtcag ccaatgcagg gacaaacaca atatcagaaa
     1201 gtacctcggc agagcgagat caacaattgt tggttaggct tgggcgagca accattttgg
     1261 tattgattgt attggtgtgg gcagtgctgg ctggatggtt ctatatttcg gcccgtagga
     1321 tggaatgata tattttggaa tgctaatccg caaataaggg tttgaactta tctccgtaac
     1381 ttgttttgcc ggctttcaat actctcaagc agcgaggtga aagatttagc gaaggcttca
     1441 accccttcgt cctctaattc cttagtgacc tgttccatcg agattcccag attttctaac
     1501 ttttccaata ccaatcgcac ttcatcaatg tcttcgctga ttgtttgccg gacttcacca
     1561 tggtcaagga acgcttccag tgtattttgt ggcacggtgt taaccgcgtg agggccaatc
     1621 aactcctgga catatagaat atcagaataa gctggatttt ttgtgctggt tgaggcccag
     1681 agagggcgtt gtacgcacgc cccttgtgat tgcagagcga taaatcgctg gctcccaaat
     1741 agttcctgaa ataggctata tgccagctta gcattagcaa ctgcagcctt gccttttaga
     1801 gacgccgcca attccgcgtg aggaccgttt tctaacagga tactatcgag gtgtttatcc
     1861 actttgctgt ctacccgaga gacgaagaac gaggcaaccg aggcgacatg gtttatcgat
     1921 ttaccggctg ctaggcgttg ctcaagtcct tttagataag catcaaccac ctggtgataa
     1981 cgagtaaggg agaagattaa agttacattg acattgatgc cactagcaat tgaggctgtg
     2041 attgctggca atccttccac ggtagcaggg attttgatca tcaagttcgg tcgatctacc
     2101 tgcaaccata atctgtgtgc ctcttcgata gtctccatgg tttggcttgc caaggttggg
     2161 tttacttcaa ggctgacata gccgtctcca ccttctgtat catcatatac cgaccgaagc
     2221 aaatcagcag ctgcctgaat atcctcaatc gccaaagcct catacacagc caatggggtc
     2281 ttaccagcct tgctaagcct ctccaggtca gagtcgtatt cccccaattg atctggcttt
     2341 gacccctgac cactcaagat agctttttca aatatggtgg ggttggacgt cattccacgg
     2401 acacccagat cgatcaaagc ctgcatttca cccgaggtta tgaaggctcg gcggatataa
     2461 tccaaccaga tcgcttgtcc aagtgctgca agttcatgta gtttggtcat taggcctcca
     2521 attactaatt gaggaaattc aaatttaggc attttttgcg gcaattttgt catcgccaaa
     2581 tacccacctt ttaccggtga atactaatga taatcgattt tccgatcgag ttcagactga
     2641 gaggaatcct tgctataatc taatttatgg atgtttcatt cccgccgcct aattttcatg
     2701 aagtaccttc aaacgttagt gggattgagg tatacgcccc aattccggag cagacgagcc
     2761 cccaacaagc tgttgtcgaa tttacttgcc ctcagtgtgg ggctacaact gcctatagtg
     2821 tggaagatgg tggtttaaaa tgctcccatt gtgggtatta ttcgccgcct gagagaaaga
     2881 caactggcgc gcaggcgagt gagcttgaat ttacggttga gacaatgcaa gacatggagc
     2941 atggttgggg agaggctcgc aaggatatgg agtgcatgac atgtggggca atcacatcga
     3001 tatctgagca gtcgttgaca cacacatgtc cattctgtgg ttctaatcat gtcattcaaa
     3061 aaacagcctc acaagacgta cttcgcccgc gttacttgat accattccaa atcgaagctc
     3121 aaaaatgcct ttctattgcc caggaatgga tgggcagcag ttggatgaca cccaaatcac
     3181 tgaaaagggt tgcggaccaa gccctgttca gcggagtttt cctgccttat tggacttttg
     3241 acactacagc tactgcatcc tggagggcgg aagttggtca tacggagact gaacgctatt
     3301 ttcaagacgg agagtggaaa acgcgcactg taatcagatg gcgttgggag tctggaaagg
     3361 tgaatgagaa atttgatgat ttactggttc ctgggacaaa taggttgagt agccttttgc
     3421 taaataagat aaaagctttc cagatgagtc agctcagacc ttatgaagct ggatttctgg
     3481 ctggatttca ggctttatct tatgaccggc ctctggataa ttcatgggag atagctcgcc
     3541 agcagatgcg agagcatacg cgcctggcat gtagaaaaca agccagtaca ccacgaatcc
     3601 gtaatttcag tatgaaactg gatttcggga atgagagctg gcggtatatt ttgctgccga
     3661 tctacttggc gggttatcgt tatgctaatc aaaacttcca ggtcatcatc aatgggcaaa
     3721 gcgggcaaat tgcggggcaa cgccccgtga attggaaaaa agtgtggttg gtagtcggtg
     3781 ctctgctcac gccaggatta cttctggcac ttatcggagc cattactttg ctacttggag
     3841 gagtaggcgc tctaatcgga ggtgttggct ttgtattgct gctcatcggt gtggtaatct
     3901 cggttgtgat ccttcgccag gctacgagta tggatgatat ctaaagaggg atatccatgg
     3961 gagaagactc gagagaactt gcccactttg agaatcttgc ttcagtttgg ggccttgacg
     4021 cagagttggt tttttgccaa aagtgtgaat ggacatattt ggctccaaaa aatcttatga
     4081 ccaagccctg tcctcactgc tttcatgagg cgcttgcgct cattgagcag gacagcagcc
     4141 aatttcctga tctaaaacct cctgagctag taatgccatt tgagatccaa gccgaagcgg
     4201 tattacgagg gattgaacgt tttgctgagg gaatcagatt tgctcctgcg gatctgaagg
     4261 cagaccagct tattaggagg cttaaacgtg tatatgttcc cgcttggtta gtagatgtag
     4321 acgtgcaagc tacttgggag ctagaggctg gtttcgatta tcaggtagtc agtcaccagg
     4381 atcgttacaa tgagatcggt gggggatgga cctcccagca ggttattgag gggcgtatac
     4441 gttgggagcc tcgggtaggt cgattgacgc gcacctacca aaatatctct gcgcctgcaa
     4501 ttgaagatga tcgagagatt c
//
LOCUS       DBNGSX010000064         3183 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_350427, whole genome shotgun sequence.
ACCESSION   DBNGSX010000064 DBNGSX010000000
VERSION     DBNGSX010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_350427"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..2066)
                     /locus_tag="AC16CB_02080"
     CDS             complement(186..2066)
                     /locus_tag="AC16CB_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3341284.1"
                     /translation="MFYTLTIIPIAFTLYDQFFANPLWYSGLPFNYTGGFIPLGEVTQ
                     GLIADRIRFVSFTLLPALALLVLLYFAIFDKRVLGENRRLAWLLLIAQAASYMTAKVL
                     SPQLLPAVAAIASSTLFITVFVYAFFLQILSERRIQRGRLQFRLTASIMVVAIPLLIA
                     ITVFVNWRASSLINWGATTQLSESELLAGEMLFQRTSLISLAIGVMLLVLISWLTIRY
                     ALQPLIDLSNTANGVASGDLTRVAVVQTEDELGDLARAYNSMITQVRETITSLQRRVA
                     ERDRDLERRTIQLQLSAEVARQSSANSGLDQLLDRLVRLISDRFNIYHAGIFLIDEEG
                     DHLVLRAASSPGGKRLVERNYKQAIDQTSLVGSVADKGELLLSQDIDKDTLAIGHADL
                     PQTRSEIALPLKTSDLTLGVLDLQASKIAAFSNDDIEIFEILSDQIALAIDRAKLQTK
                     NEELSREMGLLYQQQIHQSWQRRLEDLSMTFAYRSGNVTREFSHDAIEEYEEQKAHLL
                     KLPVILRDQSLGWVTLRRHHEGQPWSEEETDLVKETLTQIALALENARLLEENQQRAK
                     NEALVGNITGKTQGLLDVESVMKTAVREIGSSLGLTRVQITLKPDEPEPTGSNGGLEL
                     LE"
     gene            complement(2384..>3183)
                     /locus_tag="AC16CB_02085"
     CDS             complement(2384..>3183)
                     /locus_tag="AC16CB_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3341285.1"
                     /translation="LQTIANLIASHIQNLSLLRTTQSNLKVMEKRVAILETLDLVSKA
                     ISSDLDLNTLFQLAHQQINQLMGDVDVTITFEDTNINTAEVSYPAEPDGSSPKQSDIS
                     DQGLITRVLHTKSPLMLVEDAELQAAELGIPFTGAAAKSWLGAPLLVGGMAIGAIVVR
                     DLHQKHRFDEDDQRFLINLATQIAITVQNNQQLENARAQAERERIANEISSQLWSATD
                     VQSILRTAIKELSNKLGAVEGVIHIHAPNAPNGISAQANNGDNKGSP"
BASE COUNT          818 a          728 c          772 g          865 t
ORIGIN      
        1 gcttgggcaa gcagcgctgt actgctgatt gtttgagctc gatttcccga aatttggttg
       61 atcatcatgg caaccaacgc gacggatacc attgctaccg tcatcattcc caatagaaag
      121 atgagtaacc tcgtttttat gctggtgaga ggcgtaaaca agcctcgttt ttccataatt
      181 tcaggtcact ccagcagttc cagaccaccg tttgaccccg ttggctcggg ttcgtcgggt
      241 ttcagggtga tctgaactcg ggtaagcccg agcgagctcc caatttccct tacagcggtc
      301 ttcataactg attcaacatc cagcaaccct tgagtcttgc cagtgatatt tcctacaaga
      361 gcttcattct tagcacgctg ctggttttct tccaaaagcc gggcattttc aagcgctaat
      421 gctatttggg tgagtgtttc tttcactagg tcagtctctt cttctgacca gggttgacct
      481 tcatgatgac ggcgtagtgt gacccaacct aatgattggt cacgcagaat gacaggaagt
      541 ttgagaaggt gagctttttg ttcctcgtat tcttctatgg catcatgtga aaattccctt
      601 gtcacattgc cagagcggta agcgaaggtc atagacaggt cttctagccg tctctgccat
      661 gattggtgta tctgctgttg ataaagcaat cccatctcac gcgaaagttc ttcattcttt
      721 gtttgtagct ttgcgcgatc aattgcgagt gctatctggt cagaaaggat ttcaaatatt
      781 tcgatgtcat cgttggaaaa agctgcaatc ttgctggctt gcagatctaa aactccaagg
      841 gtgaggtcac tggtttttag tggcagtgcg atttcagaac gtgtttgcgg gagatcagca
      901 tgcccgattg cgagggtgtc cttgtcaatg tcttgggata ggagcagttc acccttgtcc
      961 gccacagacc ctaccaaaga tgtttgatca atggcctgtt tgtaattgcg ctccaccaat
     1021 cgctttcctc caggcgagga tgctgcgcgc aagacgaggt gatcaccctc ttcatcaatc
     1081 aggaagatac cagcatggta tatgttgaag cgatcggata tcaagcggac aagccgatcc
     1141 aagagctgat ccagaccgct atttgctgat gactgtctag cgacttcagc gcttaattgc
     1201 aactggattg tgcgacgctc aagatctcta tcgcgttcag caacacgccg ttggaggctt
     1261 gtaatcgtct cgcgaacttg tgtgatcatg ctattataag cacgcgcaag atctcctaat
     1321 tcgtcttccg tttggacaac ggccactcgg gtgagatccc ccgatgcgac gccattagca
     1381 gtgtttgata aatctattaa tggctggaga gcataacgaa ttgtcagcca tgaaatcaag
     1441 acaagaagca ttacgccaat agcaagggag attaatgacg tgcgttgaaa taacatttct
     1501 cccgccaata attcactttc agaaagctgg gtggtggcac cccaatttat caagctactt
     1561 gcacgccaat tcacgaatac cgtaatcgca atcagaagtg ggatggcgac aaccatgatc
     1621 gaagctgtta gccgaaactg cagtctgcct cgctgaatac gacgttccga taagatctgt
     1681 aggaaaaatg catatacaaa gacggtgata aatagtgtgc tggaagcaat tgcagccact
     1741 gctggtaaaa gttgcggtga caatactttc gctgtcatat aggatgcggc ttgggcaatg
     1801 agtagcaacc aggcaagccg ccgattttct ccgagcacac gcttgtcgaa aatagcaaag
     1861 tagagcaata ccaataacgc cagggctggc aacagtgtaa aagagacaaa tcggatacga
     1921 tcggcaatca gaccctgggt cacctcgcca agagggataa atccacctgt gtaattgaat
     1981 ggcaaaccac tataccagag gggattagcg aaaaattgat cgtaaagagt gaatgctatt
     2041 ggaatgatgg ttaacgtgta gaacatccac cctgtccagc gatttcgact gatgagccac
     2101 tcgggtttta caatagcgat tatggctacg aaaagcaatg gttggatgga tggagaggta
     2161 atcgctaaca agagagcagg aaagtgcgcc tgttgcaggt tttcaacccc gatcatccag
     2221 ccgactacaa atgtattgat tccaaccaag cacagtatcg cactggcatg acggttagcg
     2281 cgatcccaag cgtttaacac cagtacatac aagccgagaa tgagctcaat gatggcaatt
     2341 aaccaggcta aagattggat tgattgatcg atcatcgata gagtcacggt gatcccttgt
     2401 tatctccgtt gtttgcctgt gcagatattc catttggggc atttggcgca tgaatatgga
     2461 taaccccttc aacggctcct aatttgttgc ttaattcctt gatagcggtt cgcagaatgg
     2521 actgcacatc tgttgcggac catagttgtg acgagatttc gttagcaata cgttcgcgct
     2581 ctgcttgggc gcgagcattc tctaattgct gattattctg gactgtaata gcaatttggg
     2641 ttgccaaatt gattagaaag cgctggtcat cttcatcgaa gcgatgcttt tgatgtaagt
     2701 ctcttaccac tatggctccg atagccattc ctcctaccag tagaggtgct cccagccagg
     2761 acttggctgc cgcgcccgta aatgggattc cgagctccgc tgcctggagc tcagcgtctt
     2821 cgaccagcat tagcggcgat ttagtatgca ataccctggt aattagccct tggtctgaga
     2881 tgtcagattg ctttggtgag gatccatcag gttcagctgg atatgacacc tctgcggtat
     2941 tgatgtttgt atcttcaaac gtaatggtga catctacatc gcccatcaat tggtttatct
     3001 gttggtgggc cagctgaaat aaagtgttaa gatcgagatc ggatgagata gctttactaa
     3061 ccaaatcaag cgtctctagg atcgcaaccc ttttctccat tactttcaga ttactttgag
     3121 ttgttctcag cagagacaga ttctgaatgt gtgacgcgat caaattagcg atggtttgta
     3181 agg
//
LOCUS       DBNGSX010000065         5179 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1458600, whole genome shotgun sequence.
ACCESSION   DBNGSX010000065 DBNGSX010000000
VERSION     DBNGSX010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1458600"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="AC16CB_02090"
     CDS             <1..190
                     /locus_tag="AC16CB_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341286.1"
                     /translation="ATVNDPDGESSGIVDASAWFGPGAWLLDVQGHGANVAEEPQPDG
                     TLFKRESGQLLLMVIPGS"
     gene            541..2028
                     /locus_tag="AC16CB_02095"
     CDS             541..2028
                     /locus_tag="AC16CB_02095"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HIR3341287.1"
                     /translation="MERGKGFDEENFDYPSLPKYVYYGVGQLVYQLGYTKKEFIEAAR
                     LVSVILGGLVVALTYQLTRLIGGGITASILAALFVLSSRDMTLNSRFAHNDLFLVFFI
                     CLSVLFLIKYRLTNQRVWLYSAFFSVGLAASSKYNGGSMLLAVLVVFLISNRRLITDD
                     ILAFLEMLFIGIILSALGYAIGTPRSLTWMAFYFKRATPYILRHVAYNQEPDSLIGLF
                     GQWGVLWSMLGVGVSLLFLGAILWHGRRILILRSKIFQGENRQVELILVFLLCMIVFD
                     LPIAFSYNYQSRFFLAFIPLIAVLIGLFIEAFFAYFDSANYSIFKISSIIVLVIVLTF
                     SFLRVISVSLLFLNDNRIAASEFMTTLRKGKTLEYTLYPPTIPDEYFSEEIKHPLYFK
                     KYPNQPPPERNPSEYNRGEAGIEEREPDYFVVDSFTYDRLDQDGNCSYMKLECEFFQR
                     LFAGKTNYQLLRKFDYSVPPYLPEKSAPFLNPVIQIFQRKGTSPP"
     gene            complement(2119..2982)
                     /locus_tag="AC16CB_02100"
     CDS             complement(2119..2982)
                     /locus_tag="AC16CB_02100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR3341288.1"
                     /translation="MRKNLVSQVYEQINQFSGGALEILKRTMNSLSRERAGQAAAGLA
                     YYAFFSLFPLLLLLVVGSSFFFESDQASQQVVEFISQALPVSTTLLERNIAQVLELRG
                     PVSAIGLISLLWSATSVFSALAHNINRAWPKSKERNFFNKRLVGLLMMSALLALFGLS
                     VIVTSFNTLLSAWRIPAFGSVSKTNLWGILSQISSWFLIFLMFLALYRWVPTEDVGWK
                     PAVWGALAATFLWQIVTTAFTWFFRAGLGSYDLVYGSLGAVVALLILIYINAWVALFG
                     AHLCAAIMGEQ"
     gene            3224..3631
                     /locus_tag="AC16CB_02105"
     CDS             3224..3631
                     /locus_tag="AC16CB_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008869047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HIR3341289.1"
                     /translation="MTLSYYIKLYISALAAFLLIDAIWLGLVARGFYRKYLGFLLSPT
                     PNWAAAIIFYLLFIVGLVVFAIAPGLQAASLGKALLLGALFGLLTYATYDLTNLATIK
                     DWPLIVTVVDLVWGVILSSSVTWVAFQVGRWLG"
     gene            3661..5070
                     /locus_tag="AC16CB_02110"
     CDS             3661..5070
                     /locus_tag="AC16CB_02110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3341290.1"
                     /translation="MIETQKHKSPIHRSFWLEQVAGDAPEEALLMGDQRADVAIIGGG
                     YVGLWTALRIKEIDAGCDVVVLEQDICGGGASGRNGGFVLSYWPKLASLAKICGEQDA
                     IQIARDSEEAIDSIRDFCEAYQIEAHFRRGGWIWTATSQSQMDSWDSVVRLCDKLGVD
                     AFQELSPQEVAQRSGSPAHRAGVFEASAATVQPAALVRGMRRVALQRGVRIFEGTRVQ
                     AFGRTHPIAIKTNAGVLTAEKLVIASNAWAAGIRELSRSIVAVTSDMVITAPVPDELQ
                     NLGWVGGECITDSLMMVDYYHATQEGRVAFGKGGWGIAYGGNIGKDFDRNEQRAQNVI
                     AHLHRYYPSLRQVPLTHDWSGPIDRTPNSLPVLGRLGGRNHILYGIGWSGNGVGPSVI
                     GGRILASLALERDDDWSRYPLVEWSARRFPPEPIRYIGAHFVRNAVVQKEWAEIRDQK
                     PPYLAVKLSKLAPSGIEDK"
BASE COUNT         1201 a         1256 c         1358 g         1364 t
ORIGIN      
        1 agccaccgtt aacgaccccg acggcgagtc cagcggcatt gtggacgcct ccgcctggtt
       61 cggccctggc gcctggctgt tggatgtgca gggtcatggg gcgaatgtgg ctgaggaacc
      121 tcaaccagac ggcacgctct tcaaacggga atcaggccag ttgctgttga tggtgatccc
      181 tggttcgtag ttaaaaaaga atatccttga agttgagccg ccttcatcac catgaaggcg
      241 gctctgtttg tgcgctatgg ttgcttccct gtcggcgagc attctataat ggtgaacagc
      301 gatccaaact gttataatcg tgcatcgagt ttcctatcga agtacataaa cctttgttgg
      361 gatgttgtgc tctaaatcat ggcttatcgg tctcaactca cttctgaatt acacaaatac
      421 aataagttta tcccggttgc ttttttcttg gtctttgtcc tgatcactat cccaggaatc
      481 tcttggggcg cgcctgggtt ctggcatccg gatgaaattg tgcaagccgc ggaccgagcg
      541 atggagcggg gcaaggggtt cgatgaggaa aattttgact atccgtcctt gccaaaatat
      601 gtttattatg gggtgggcca gctcgtctat cagttgggtt acacaaaaaa ggagtttatt
      661 gaggcggcgc gattggtctc ggtcatattg ggcgggctgg ttgttgcgct gacctatcag
      721 ttgacccgat tgataggtgg gggaataaca gctagcatct tggcggcatt gtttgtgcta
      781 tctagtagag atatgacgct caactcccgc ttcgcccaca atgatctgtt tctcgtcttc
      841 tttatttgtt tgagtgtgct ttttctgatc aaatatcgcc tcacaaacca gcgggtttgg
      901 ttgtatagcg ccttcttcag tgttgggttg gcagcttcga gcaaatacaa tggcgggagc
      961 atgctgctcg ccgttttggt tgtttttctg atctcgaata ggcgcctgat aaccgatgat
     1021 attttagcat ttttggagat gttatttatt ggaatcatcc tgagcgcttt gggttatgct
     1081 attggtacac caagatcgtt aacttggatg gcgttttatt tcaaacgagc caccccatat
     1141 atcttgcgcc atgtagcgta taaccaggag ccggatagcc tgattgggtt attcgggcaa
     1201 tggggtgtgt tgtggtccat gttgggggta ggggtttccc tcttgttctt aggtgcgatc
     1261 ctgtggcatg gccgcaggat tttaatactt cgttcgaaaa tcttccaagg cgagaacagg
     1321 caggtagaat taatcttggt atttcttctc tgtatgatcg tattcgatct gccgatcgcc
     1381 ttttcctata actatcaatc cagatttttt cttgccttca tcccactaat tgctgtactg
     1441 attgggttgt tcattgaagc attctttgcg tatttcgatt ctgcaaatta ctcaattttt
     1501 aaaatctcga gcattatcgt cttagtaatt gtcttaacct tttcttttct tcgcgttatc
     1561 agcgttagtt tattgttttt gaacgataac cgtattgcgg ccagtgaatt catgacaaca
     1621 ttgcgaaagg gtaaaaccct tgaatatacc ttatatcccc ccactatccc tgatgagtat
     1681 ttctctgagg agataaagca ccctttgtac tttaagaaat atccaaacca accgcctcct
     1741 gagagaaatc catctgagta caatcggggg gaggcaggga ttgaagaaag agagcccgat
     1801 tattttgttg tcgatagctt cacctacgac cgcttagacc aagatggaaa ttgcagttat
     1861 atgaagttgg aatgcgagtt tttccagcga ttgtttgctg gaaaaaccaa ctatcagtta
     1921 ctaagaaaat tcgattattc cgtaccgccc tatttgccag agaaaagcgc ccctttcttg
     1981 aatccagtta ttcagatatt ccagagaaaa gggacatctc ctccatgagg gtggaggtcg
     2041 gtccggataa acttgcttgt gaccgtaaat aaacccatta tagagtttag gcaatgagcc
     2101 attaaaaggg atagcaattt attgctcacc catgattgcc gcgcacagat gggccccaaa
     2161 taacgccacc caggcattga tgtagatcaa aatcaatagc gctaccacag ctcccaggga
     2221 gccatagacc aaatcgtagc ttcccaagcc agccctgaag aaccaggtga aggcagttgt
     2281 aacaatttgc cagagaaatg tggctgccaa tgcgccccat acggctggct tccagcctac
     2341 gtcttccgtg ggcacccagc ggtacaaagc cagaaacatc aagaatatta ggaaccaaga
     2401 agaaatctgt gagagtattc cccacaagtt ggttttggat acgctgccaa aggcaggaat
     2461 gcgccatgcg gagagcaatg tattaaagga tgtcactatc accgataacc caaaaagcgc
     2521 caacaaggcg ctcatcatca gcaaccccac caggcgctta ttaaaaaaat tacgctcctt
     2581 cgattttggc catgcccggt tgatattgtg agccaatgcc gaaaacacgc tagttgcaga
     2641 ccataacagg ctgatcaagc cgatggcgct aaccgggccg cgcaattcca gcacctgagc
     2701 gatatttctc tccaggaggg ttgtggatac gggcagtgcc tggctaataa attcgaccac
     2761 ctgctgcgag gcctgatcgc tctcgaagaa gaagctgctt ccaactacga gcagtagcaa
     2821 gagcggaaac agtgagaaaa aggcatagta tgccaacccg gctgcagctt gaccggcgcg
     2881 ctctcggctc aaactgttca ttgtgcgctt caagatctcg agcgcaccac cgctgaactg
     2941 gttgatctgt tcataaacct gtgacacaag gttttttcgc atactcgcca tttcctgatt
     3001 cgtaaatatt acgttcgacg tataatatct attaggcgtt tcaagtcatc gtcttgacgg
     3061 tgcgtgaaaa atctaacgtt tgctgtatgg cgtgctctgc tttaggtttg gtgcgaacca
     3121 atgacttatc aatacgattc ggccctgaat tgattgccag cgcacgtcca tacttccaaa
     3181 gccctagcta tccaagctct atgtaagacc taatggaggc ctcatgactc tatcttacta
     3241 catcaagctc tacatctcag ccctggctgc cttcttgctc attgatgcga tttggttagg
     3301 cctggttgca cgcggatttt accgcaaata tttagggttc ctgctcagcc cgacccctaa
     3361 ttgggcagca gccatcatct tttatctgtt atttatcgta gggctggtgg tgtttgccat
     3421 tgcaccggga ttacaagccg catcgctggg caaagccctg ttgctgggcg cgctgttcgg
     3481 cttgctcacc tacgccacct acgacttgac caatctggcg accatcaagg attggccttt
     3541 aatcgtcacg gtggtagatt tggtatgggg ggtgatcctc tccagttccg tgacctgggt
     3601 ggctttccag gtcggcaggt ggttgggata aatccttgtt gagggaggcc acccaaggct
     3661 atgatcgaga ctcaaaaaca taaatcacca atccaccgct cattttggct cgagcaggtg
     3721 gcgggtgatg ccccagaaga agcgcttcta atgggcgatc agcgtgccga tgtcgctatc
     3781 atcggtggcg ggtacgtagg tttgtggact gcactccgca ttaaagaaat agatgcaggt
     3841 tgtgatgtgg tggtgttaga gcaggatatt tgtggagggg gcgcttcggg tcgcaacggt
     3901 gggtttgtgc tgtcttattg gccaaagctc gcctcgcttg ccaaaatctg cggtgagcaa
     3961 gacgccatac aaatcgcgcg agattctgaa gaagctattg attccatccg tgacttttgc
     4021 gaggcctatc aaatcgaggc gcattttcgg cgcggcggct ggatatggac tgccacctcc
     4081 cagtcgcaga tggactcctg ggacagcgtg gtgcgcttgt gcgacaagtt gggagtggat
     4141 gcctttcaag agctctcccc gcaagaggtc gctcagcgct cagggtcacc tgcgcatcgt
     4201 gcaggtgttt ttgaagccag tgccgccacc gtccagcccg ctgctctggt gcgtggaatg
     4261 cgccgggttg cgctccagcg tggcgtgcgc atttttgaag gcacgcgcgt ccaggccttc
     4321 ggccgaacac atcccatcgc catcaaaacc aatgctggag tgttgaccgc cgaaaagctc
     4381 gtcatcgcct ccaatgcctg ggcggcgggc atccgcgagt taagccggag cattgtcgcc
     4441 gtcaccagcg atatggtgat taccgcccct gttccagatg aactgcaaaa tctgggctgg
     4501 gtgggcggcg agtgcatcac cgattcgctc atgatggtcg attactatca cgccacacaa
     4561 gaaggtcggg tcgcttttgg taagggcggc tgggggattg cctatggcgg caatattggc
     4621 aaagatttcg accggaacga gcagcgtgct cagaacgtca tcgcccatct gcaccgttac
     4681 taccccagcc tgagacaggt gccccttacg cacgattggt ctggccccat tgaccgcacg
     4741 cccaacagtc tgccggtgct tgggaggctg ggtgggcgca atcacatcct gtacgggata
     4801 ggctggagcg ggaatggagt cgggccgagt gtaatcggtg gaagaatcct tgccagcctt
     4861 gcgcttgaga gggatgatga ttggagtcgc tatccgcttg tggagtggtc tgcaaggcga
     4921 tttccacctg agccaatacg ctatatcggc gcacactttg tgcgcaatgc tgtggtgcaa
     4981 aaagaatggg ccgagatacg tgaccaaaag ccgccttacc tggcagtcaa gctatcaaag
     5041 ctagcgcctt cgggcattga agacaagtga tgcgcgcggg tgataagtgg aggatatgcg
     5101 atgaaagaac ccggaaacaa cccgttacca tccatcctgg tggtaatcat tttgctggga
     5161 ttgatttacg ccttatatg
//
LOCUS       DBNGSX010000066         3421 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1804783, whole genome shotgun sequence.
ACCESSION   DBNGSX010000066 DBNGSX010000000
VERSION     DBNGSX010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1804783"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /gene="recF"
                     /locus_tag="AC16CB_02115"
     CDS             complement(<1..1125)
                     /gene="recF"
                     /locus_tag="AC16CB_02115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.2"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIR3341291.1"
                     /translation="MFLKRLSLTNFRNYARLDVDVPTGSVLLVGDNAQGKTSLLEAVY
                     FLATFVSFQADSDRQLINFIAGREPLAVARIVADFQRQEMDHSSRDHRLEVRIIQEAN
                     GINGGSRVRKEVLLDGVERKISEAVGVFNAVLFQPQTLRIIEGAPEDRRRYLNLTLAQ
                     AVPRYTAALTDYNRAITQRNALLKLLNERGGDPNQLTYWDELLASSGASLIYARIQAI
                     KGLERLAARIHSQLTQGAEVLRLSYLPAYDPLPKPERQYALPIDAPLDRSGIPLEKIQ
                     QGFFESLERLHSEEIARGVTTIGPHRDELRFLANGIDLGVYGSRGQSRTAILALKLAE
                     VAWLKEETGQWPVLLLDEVLAELDVQRRTDLLERLADIEQA"
     gene            complement(1125..1637)
                     /gene="def"
                     /locus_tag="AC16CB_02120"
     CDS             complement(1125..1637)
                     /gene="def"
                     /locus_tag="AC16CB_02120"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR3341292.1"
                     /translation="MAIREIITLPSSTLRRKARKVTSFGPQLQSLIDDMVETMREAPG
                     VGLAAPQVDVSWRVIVVEFGDEEDEEAPKKLYTVVNPEISRASQEMSVGTEGCLSIPG
                     FVGEVERPDAITIKGLNRYGKPFRINADGWLARIFQHEVDHLEGVLFIDRAEKVWKVE
                     EADAEVAAAD"
     gene            complement(1674..3374)
                     /locus_tag="AC16CB_02125"
     CDS             complement(1674..3374)
                     /locus_tag="AC16CB_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR3341293.1"
                     /translation="MVDPDISKYASLLKQQYLFQGLNDAQLAHVVTHVERIEKFKDDV
                     IYAQGSPGDKFYVIFQGRVRVTQKEGQRERQLRILNAGDYFGEGGLLFKRPRSDTITA
                     LEPTVLLRLSREDFFELLEIMPSIRMNLSATAESRYLALKENFDWLSDDEVIYLIRRK
                     HELFLLISLIPPVILGLIALPIFVLSFSQTSPSLTIAALLFGIFGMIGSVMWGIWNWL
                     DWGNDFYIVTSQRVVWLERVIFFYYSRQEAPLTQVLSVNVKTSFIGRIFNYGSVDVRT
                     FTGGIPMQNMTDPKQFASYVEGFQSRAQRQQREAEAEVIEKELHKRIHKESEPLPVKP
                     APPKPAENKKKPKQSALQYILDTFLKVRFEQDNVITYRKHWLVLIGKTLAPTMVFLML
                     VILYGLLLYQYLFRDGIGISGLPVSVLAGLLLFAVILWWGYHYLDWNNDIYQLTPEQI
                     VDIERKPLGEEQKKTAPLDSILSLEHARLGIIQLIFNFGNVVINVGQTKFIFRGVHNP
                     DQVHQDVADYIEARRRKRQEVEAERERKRMLDWFGSYHQQHDEIEQSKKDTDWDLFPG
                     "
BASE COUNT          829 a          853 c          866 g          873 t
ORIGIN      
        1 agcctgttca atatctgcca agcgctctaa taggtcagtg cggcgttgta catcgagctc
       61 agccaggact tcgtctaaca agagaactgg ccattgaccg gtctcttctt ttagccaggc
      121 cacctcggct agtttgaggg ccaggatggc ggtgcgcgac tggccgcgtg aaccgtatac
      181 ccccaaatca attccatttg ctaagaagcg taattcgtcg cggtggggac caattgtggt
      241 gacaccgcgg gcgatctcct cagaatgtaa gcgctccaga ctctcaaaaa aaccttgctg
      301 gattttttcc aatgggatgc cggagcgatc gagtggcgca tcgatgggta atgcgtattg
      361 gcgctcaggt tttggcagtg gatcgtaggc tggcaggtag gatagcctta atacttcggc
      421 tccctgtgtc agctggctgt ggatgcgtgc ggccaggcgc tccagccctt tgatagcctg
      481 gatgcgggca tagatcaagc tggctccgga agaagccaat aattcatccc aataggttag
      541 ctggttgggg tcgccgccac gctcattgag cagttttagc aaggcgttgc gctgggtgat
      601 ggcgcgatta taatctgtca gtgcagctgt gtagcgggga acagcctgag ccagggtcag
      661 gttgagatag cgacgacggt cttcaggggc accttcgatg atacgaaggg tttgcggctg
      721 aaagagcacg gcattgaata cccccactgc ctcgctaatt tttcgttcga caccatccag
      781 gagcacctct ttccgcacgc ggctaccacc attaattcca tttgcttctt ggataatgcg
      841 gacctccaga cggtggtcac gagaagaatg atccatctct tggcgctgaa aatccgccac
      901 gatacgcgcc acggctagcg gctcgcgccc ggcgatgaaa ttgatcaatt ggcggtcgct
      961 atcggcttgg aatgaaacaa aggttgctaa aaaatataca gcctctagga ggctggtctt
     1021 cccctgggcg ttatcgccca ccaaaagcac cgaaccggtg ggcacgtcta cgtccaggcg
     1081 agcgtagttg cgaaaattcg ttagcgaaag gcgcttgaga aacattaatc tgcggcggct
     1141 acttcagcgt cggcttcttc gaccttccag actttttccg cccgatcaat aaacaggaca
     1201 ccctctaagt ggtcgacctc gtgttggaaa atacgggcaa gccagccatc agcatttatc
     1261 cgaaatggct tgccataacg atttaatccc ttaatcgtga tggcgtcggg gcgttccacc
     1321 tcaccgacaa aaccagggat ggagagacaa ccttccgtac caacagacat ctcctgggat
     1381 gcgcgagata tttctgggtt gaccacggtg tagagctttt tcggagcttc ttcatcctcc
     1441 tcatcgccga attccacaac aatgacccgc catgagacgt ctacctgcgg cgcggccaag
     1501 cccacacccg gggcttcgcg catggtctct accatgtcat caataagcga ctgcagctgt
     1561 ggtccaaaac tcgttacctt gcgagctttg cgtcgcaagg tggaagacgg aagggttata
     1621 atctcacgga tagccatact tacctctaaa caaatctaaa ctgaagtact attttacccc
     1681 ggaaaaaggt cccaatcggt atcttttttc gattgttcta tttcatcatg ttgttgatgg
     1741 tagctgccga accaatctag catacgcttg cgttcacgtt ctgcctccac ctcctggcgt
     1801 tttcggcggc gggcttcgat ataatctgcc acatcctggt gaacttgatc gggattgtgc
     1861 acgcctcgaa aaataaattt ggtctgccca acgttgataa ctacgttgcc aaaattgaag
     1921 atcaactgaa tgattcctaa acgggcatgc tctaagctaa ggatggagtc taagggtgca
     1981 gtttttttct gttcctcgcc caatggcttg cgttcaatat caacgatctg ctcaggtgtg
     2041 agctggtaga tgtcgttatt ccagtctaaa taatgataac cccaccagag gattactgca
     2101 aacaagagca accctgccaa gacgctaact ggtaaacctg aaattccgat tccatctctg
     2161 aaaagatatt gataaagtaa taaaccatag aggatcacca gcatgaggaa taccatagta
     2221 ggcgccaggg tcttgccaat caaaaccaac cagtgtttgc ggtaagtgat cacgttatct
     2281 tgttcgaatc gcaccttcaa gaaagtatcc aggatatact gcagggcgct ttgttttggt
     2341 ttctttttat tctccgctgg cttgggagga gctggcttaa ctggcaatgg ttcagattcc
     2401 ttatggatgc gtttatgcag ctctttctca atgacctctg cttcggcttc tctttgctgg
     2461 cgctgggcgc gcgattggaa tccctcaaca taggaagcaa attgtttggg gtctgtcata
     2521 ttttgcatgg gaatgccacc cgtgaaggtg cgtacatcga cactcccata attaaatatt
     2581 cgcccaataa agcttgtttt gacattgaca gagagcacct gtgtgagcgg ggcttcctgg
     2641 cggctgtagt aaaaaaagat cactctttcc agccatacca cgcgctggct ggtgacaatg
     2701 tagaagtcgt ttccccaatc tagccaattc caaattcccc acatgaccga accgatcatc
     2761 ccaaatatgc caaaaaggag cgcagcaatt gtcagggaag gactggtttg cgagaagcta
     2821 agcacaaaga tcggtagcgc aatgagtccc agtatcactg gtgggattag agagataagt
     2881 agaaacaact cgtgcttgcg tcgaattagg tagatgacct catcatcgct caaccagtcg
     2941 aaattctctt tcagagctag gtagcgactc tcggcggttg cggagaggtt catgcggatg
     3001 ctcggcatga tttcgagcaa ttcaaagaaa tcttcacggc tcaggcgaag taataccgtg
     3061 ggctctaggg cagtgattgt gtctgatcgt gggcgtttaa atagcaagcc accctcgcca
     3121 aaataatctc ctgcgttaag tatccttaat tggcgctccc gttgaccttc tttttgtgtg
     3181 acacgcaccc tgccctggaa gatcacgtag aatttatctc caggcgatcc ctgtgcataa
     3241 atgacgtcat ctttgaactt ttcgatgcgc tcaacatggg tgacgacgtg ggctagttgg
     3301 gcgtcgttta gcccttggaa caggtattgt tgcttcaaca gggaagcata tttggagata
     3361 tcaggatcga ccatgcagtg attttagcac gaacttttct tgtattgggc gattcgattt
     3421 t
//
LOCUS       DBNGSX010000067         8313 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1389265, whole genome shotgun sequence.
ACCESSION   DBNGSX010000067 DBNGSX010000000
VERSION     DBNGSX010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8313)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8313)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8313
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1389265"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="AC16CB_02130"
     CDS             complement(<1..710)
                     /locus_tag="AC16CB_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3341294.1"
                     /translation="MSMLVVSDLSRHFGGLTAVQNLSFSIEPGEIVGLIGPNGAGKTT
                     TFNLLTGFTPPSSGEITFSGKLLNGLKPHDICQLGLTRTFQIVQPFPDMTVLENVMVG
                     AYVRRANPAETRRKAMQVLERVGLSHKSDILGRELTLLELKRLEIGKALATEPSLLLL
                     DEVAAGLNTVEVDDVLVLVRQLSQEGITLLIVEHLMKVIMNLSHRIIVLDFGQKIAEG
                     TPEEISRDPAVLEAYLGE"
     gene            complement(707..1705)
                     /locus_tag="AC16CB_02135"
     CDS             complement(707..1705)
                     /locus_tag="AC16CB_02135"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3341295.1"
                     /translation="MNALRNSSAYLILIAVILLIVPLFFTEYALHIAVLVFLYAYLAT
                     SWNILGGYAGQHSLGHSLFLGIGAYTSTFLFTKLGVTPWLGMWVGAAFAGLVGWFVGY
                     LCFRYGLKGPYFALVTIALAEAAVYLGNNIEFIGGAQGLEVKWEGHQPALMQFEGKAG
                     YYYIILIMTGLAIWLTHWYSRQRFGYRLVAVRENEDAAEALGVNTLRTKVQASILSAC
                     LTALGGTFFAQYFTYISPRNVFGEAPSIQILLFAIIGGLGTVWGPAVGALVLVPIAEL
                     ARSWLGGSFAGAHLLLYGTVMVIVMLFMPKGIMGLVQNSRARLANRRLPTTESEAA"
     gene            complement(1702..2580)
                     /locus_tag="AC16CB_02140"
     CDS             complement(1702..2580)
                     /locus_tag="AC16CB_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3341296.1"
                     /translation="MILSFELLAQAVLTGILIGGIFALISVGLSLIWGVMRIINFAHG
                     EFLMIGMYISYLLTAKAGWDPYLTVLVTTPLLFLVGVVIFQVSIRPILDHPSMNQIML
                     TLGLSLILQNLALVIFKADVLVTSTTYTRMTFTVGPVVVRVAQLIAFLGSGLAAILLY
                     WFLQKTDIGRAIRAAAQNRDAAVLVGINVQRTYLLAFGIGSACLGVAASFMIPFYYTS
                     PTVGLFFGLIAYVVVVLGGLGNFMGALVAGLIIGLTESVGASLLPGSLARVLTFGIFV
                     LVLLFRPQGIFGGKQR"
     gene            complement(2656..3924)
                     /locus_tag="AC16CB_02145"
     CDS             complement(2656..3924)
                     /locus_tag="AC16CB_02145"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3341297.1"
                     /translation="MKRNVLFSLIAVLMVLSMLLSACGGGAATQAPADTEAPPAETEA
                     PQAVDKVRVGAMYPLTGDLAKLGEENKNGLQLAIDEINAAGGIKALGGAQIELMWGDS
                     QGKPEVGISEVTRLVQQENVVAIIGAYQSGVTVPSTQEAERLATSFIVSMAVSDKITE
                     RGFKYTFRICPKASWYAKDQVTFLKDLKDLAGLDIKRVALLHEDTDFGESTAAGQKQY
                     LEEAGMEMVLEVKYPASAADLTTEVSKVKAANPDAVLTVTYLNDSILIARAREALGMT
                     NIPFIDAAGGTVDPEFIKQLGQTAEGWFTEIEYTKYAAGAEDLNNRYVAVFGDDITGN
                     GAYAYQAGYVIADALERAGSVDRDAVRDALAKTDLPSGPSMVLPTDHIYFDENGQNPN
                     APLFIVQVQGGELIPVWPGAYAAKPVQLSK"
     gene            complement(4056..5084)
                     /locus_tag="AC16CB_02150"
     CDS             complement(4056..5084)
                     /locus_tag="AC16CB_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009431001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR3341298.1"
                     /translation="MNVGNRYGEHRVIYPPGALPQAAQRLDNQAPPLDNEIGITVQAL
                     HITSTSFYRLWNEAYEDANNLANKIKEIVSSRGKFQDPVTGSGGVLKGVVDFVGPALN
                     IALKVGDPIVTMVSLALTPLRLDQVYDVHPASGQVEVQGYAILFESGLWAILPSDLPV
                     PLAMIALDVAGAPAYAARHVRAGQRILVIGGGKAGMLCLHEIKKHAGSAGQVILVEKN
                     ADRCQAALELGLADEALNLDATRALDVFSQINNITNEKLADVSFNCASLPGTEMATIL
                     STRDDGEIFFFSMATEFSRAALGAEGVGKPVRMIIGNGYLPGHAEIALRILRENKPLR
                     DYLSRSLA"
     gene            complement(5081..5908)
                     /gene="kce"
                     /locus_tag="AC16CB_02155"
     CDS             complement(5081..5908)
                     /gene="kce"
                     /locus_tag="AC16CB_02155"
                     /EC_number="2.3.1.247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019475 - L-lysine fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage enzyme"
                     /protein_id="HIR3341299.1"
                     /translation="MQPLIITAAVVGAELSRQDTPFLPLTPDEIAEEAWEAQQAGAAM
                     VHLHARAADGSPTQDIQAYQSIIQAVRSRCPDLIIQVSTGGAVGMSAEERLQPLALQP
                     DMASLTTGTVNFGEEVFYNSLPMIRQFARAMLEKNIRPELEIFDSGMIANALRLFKEG
                     LLLEPLHFNFVLGVPGGAAGTLKNLMHMVESVPPSSTWCASGIGASQLPLTTMAILLG
                     GHVRVGLEDNIYYRRGELSQGNAPLVARVFRLAGELGRPLATPNEARRILNLEREKA"
     gene            complement(5912..6349)
                     /gene="kal"
                     /locus_tag="AC16CB_02160"
     CDS             complement(5912..6349)
                     /gene="kal"
                     /locus_tag="AC16CB_02160"
                     /EC_number="4.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019475 - L-lysine fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminobutyryl-CoA ammonia lyase"
                     /protein_id="HIR3341300.1"
                     /translation="MSNTADTSSISAAPILTVKLRLRMSEHDVHYGGGLVDGARLMAL
                     FGDAATELCIRHDGDEGLFRAYNRVEFLRPVHAGDFLEITAHLVGVGRTSRQMRFLVH
                     RTICSAYSTENPSAAEVLSEPELVAIAEGTCVVPFDKQRRREG"
     gene            complement(6385..7830)
                     /locus_tag="AC16CB_02165"
     CDS             complement(6385..7830)
                     /locus_tag="AC16CB_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020266902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3341301.1"
                     /translation="MTEKFYHLINGEKVEGSDWFERRNPANTDEVVGLFPKGNQETAR
                     HALEAAEAAFPKWAATPPPARGDVLSRAAEIIHQRLDELAELMTREEGKTFAESRAEV
                     ARARDIFRYYSAEGRRISGETLPADKEKSILFTRREPLGVVSIITPWNFPMAIPAWKL
                     GPALVAGCTVVFKPASLAPLCALRLVEILHEAGLPKGVLNFITGSGREVGDVLVTSPT
                     VRGISFTGSYPVGHRIHQSAAPGMARTQLEMGGKNPLVVLDDADLNLAVNLAAAGGFG
                     LTGQACTATSRVIVQRGILPEFSSRLVERARSIVVGNGMQGAHMGPAVDEAQLQTDLE
                     YIDIGKSEGAQPLLVGGSIKGSTPGFFVSPTIFADVNPEMRIAQDEIFGPVIGIMPVE
                     SMEEAISYANRIEFGLSAGLVTNNLRHALVFAENIEAGVVKINQTTGGVAIQAPFGGF
                     KKSSSNTFREQGKTAVDFYSRTKSVYIDYPA"
     gene            complement(7850..>8313)
                     /locus_tag="AC16CB_02170"
     CDS             complement(7850..>8313)
                     /locus_tag="AC16CB_02170"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HIR3341302.1"
                     /translation="LALGVGNDKQYQRLCDVAGRTDLRDDARFQTNPGRVTFRDELIP
                     KLQEVFRSRPTATWIEQLSAMGIPVGPINDIPTVMQHPQVQARQMVQIIQRPDGKDVP
                     QLGPVAKYSATPARIYRHPPALGEHSQEILRQDLNLADHEIEELILEKVIA"
BASE COUNT         1927 a         2237 c         2228 g         1921 t
ORIGIN      
        1 tcctctccga gatacgcttc aagcacggcg ggatcgcggc tgatctcttc cggtgtgccc
       61 tcggcgatct tctggccgaa atccagaacg ataatgcggt gcgacaggtt catgatcact
      121 ttcatcaagt gctcaacgat aagcaaagtg atgccttcct ggctcaattg acgcacaagc
      181 accaggacgt catccacttc aaccgtgttg agtccagcag caacttcatc caacaacagc
      241 aagctgggtt cggtagccaa ggctttaccg atttccagcc ttttgagttc gagcagggtg
      301 agctcacgac ccaggatgtc agatttatgc gatagtccga cgcgctccag cacttgcatg
      361 gccttccgcc gagtttctgc gggattagcg cgacgcacat aggccccaac catcacattt
      421 tccaggacag tcatatctgg aaatggctgc acgatctgga atgtgcgcgt caggccgagc
      481 tggcagatat catggggttt gagaccattt agtagtttgc cagaaaaagt gatttcgccg
      541 gagctgggtg gggtgaaacc ggtcaggagg ttaaatgtgg tcgttttccc tgcaccattt
      601 gggccgatca gccctacaat ttctccaggc tcgatggaga atgacaggtt ttggactgca
      661 gtgaggccgc caaagtgacg gctcagatcg gagacaacca gcatgctcat gctgcctcgc
      721 tttctgttgt tggcaagcgc ctgtttgcaa ggcgcgcccg gctattttgc accagtccca
      781 tgatgccttt tggcataaac agcataacaa tcaccatgac cgtaccataa aggagaagat
      841 gtgcgccggc aaaactgccg cccaaccagg agcgcgctag ctcggcgatg ggcaccagca
      901 ccagcgcgcc aaccgccgga ccccacacgg tacccagtcc gccgataatg gcaaagagca
      961 atatttggat tgagggagcc tctccgaaga catttcgcgg actgatatac gtaaagtatt
     1021 gagcgaaaaa tgtcccacct aaggcagtca agcaagcgct taaaatggaa gcctgtactt
     1081 ttgtgcgcag ggtattcact cccaatgcct cggcagcatc ttcattttcg cgcacggcga
     1141 ccaatcggta gccaaaacgt tgacggctgt accaatgtgt caaccagatc gccagcccgg
     1201 tcataatcag gataatatag taatagcctg ctttaccctc gaattgcatc aatgcaggtt
     1261 ggtgaccttc ccatttcacc tctaatcctt gtgcgccccc gataaactcg atgttgttgc
     1321 ccaggtatac tgctgcttca gccaaagcga tggtcactag ggcaaagtac ggacctttca
     1381 gaccatagcg gaagcataaa tatccgacga accaacctac gaggcctgcg aaggctgcgc
     1441 ccacccacat gcctagccat ggagtgacgc ccaatttggt gaacagaaag gtggaggtgt
     1501 aagccccaat ccctaagaat aggctgtgtc caagagagtg ttgcccggcg tatccaccca
     1561 gtatattcca tgaagtggct aggtaagcgt agagaaacac cagtaccgcg atatgcagtg
     1621 catactccgt gaaaaatagt ggcacaatga gcaagattac tgcgatgagg atcaaataag
     1681 ctgacgagtt acgcagagca ttcatcgctg tttacctcca aaaataccct gtgggcggaa
     1741 tagaagcaca aggacgaaaa taccaaacgt gagtacgcgc gctaatgagc cagggagcag
     1801 tgaagctccc acggattcgg tcaggccgat gattaatccg gcgactaaag cgcccataaa
     1861 gttgcctagc ccgcccaaca caacaaccac ataagcgatc agaccgaaaa agagtccaac
     1921 cgtcggcgag gtgtaataaa acgggatcat aaacgacgcg gcgacaccca ggcacgcaga
     1981 accgattccg aaagctagca ggtaagtgcg ctgcacgtta atgcccacca gaaccgcggc
     2041 atctctgttc tgggcggctg cccgaattgc gcggccgata tctgtctttt gtaggaacca
     2101 gtaaagcaaa atggctgcca gacctgagcc cagaaaggca atcagctggg caacccggac
     2161 taccactggc ccaacagtga aggtcatgcg cgtgtaggta gttgatgtaa ccagcacatc
     2221 ggccttgaag atgaccagcg ccagattttg caagattagc gacagcccca atgtcaacat
     2281 gatctgattc attgagggat ggtcaagaat ggggcgaatg ctcacctgga aaatcacgac
     2341 tcccacgaga aaaagcaagg gtgtggtgac caggacggtc aagtagggat cccaccctgc
     2401 tttggctgtc aatagatacg agatatacat gccgatcatt aaaaactcgc catgtgcgaa
     2461 gttgataatg cgcattacac cccaaatcaa gctcaaacca accgatatta atgcaaaaat
     2521 tccgccgatt aatattcctg tcagcacagc ttgagccagg agttcaaacg agaggatcat
     2581 ttggcctcca attaccagtg aaataaaggt tggttcgggc agcctagccg cccgaaccaa
     2641 ccaatcgtca tgaaattact tgctaagttg gactggtttg gctgcatagg cgcctggcca
     2701 gaccgggatc agttcgccac cttgcacctg gacgatgaac aacggggcgt tcgggttctg
     2761 accgttttca tcgaaataga tgtggtcggt cgggagaacc atgcttgggc ctgatggcaa
     2821 gtcggttttt gccaatgcat cacggactgc atcgcggtcg actgagcccg cacgctcgag
     2881 tgcgtcggcg atcacgtagc cagcctgata tgcgtaggcg ccgttaccgg tgatatcgtc
     2941 accaaatacg gctacatagc ggttgttcag atcttctgct ccagcggcgt acttggtgta
     3001 ctcgatctcg gtgaaccagc cttcggctgt ttgccccagc tgcttaatga attcggggtc
     3061 taccgtacca ccggcagcat cgatgaaggg gatatttgtc atacccaagg cttcacgcgc
     3121 gcgcgcaatt aggattgagt cgttgagata tgtaaccgtc agcactgcgt ccgggtttgc
     3181 agctttgact ttggatacct cggtggtcag gtcggctgcg ctggcggggt actttacctc
     3241 cagcaccatt tccatgccag cttcttccag gtattgcttt tgtccggcgg cagttgattc
     3301 accaaagtcg gtgtcttcgt gcaacaatgc cacccgcttg atgtccaagc cggccagatc
     3361 tttcaggtct tttaagaaag tgacctgatc ttttgcatac cagctggctt tggggcagat
     3421 acggaaggtg tacttgaagc ctcgttcggt gatcttgtct gagaccgcca tgctgacgat
     3481 gaaggaagta gccaggcgtt cggcctcctg ggtggagggg acggtcacac cggattggta
     3541 tgcgccaata atggcgacga cattctcttg ttgtaccaat cgtgtaactt cgctaattcc
     3601 aacttcaggc ttgccttggg aatcgcccca cataagctca atttgagcgc cgccgagtgc
     3661 tttgatccca cctgctgcgt tgatttcgtc gatagcaagt tgaagaccgt tcttgttttc
     3721 ttcacccagt ttggctaggt cacccgtcag tggatacatc gcacccacac gaaccttatc
     3781 cactgcttgt ggagcctctg tctctgctgg cggagcctca gtatctgccg gtgcctgtgt
     3841 ggctgcccca ccaccgcatg cgctgagcag catagacagc accatcaaga cagcgatcaa
     3901 ggaaaacagg acatttcttt tcattgcatc ctccggttaa atgtaagata tggtgtttta
     3961 caatggatca cgaactttgg gtggcagtaa gatgtgtcaa tatgattatt tgacctcctt
     4021 ttctattaga ttcgggaagc ctcaagatat tcgtttcatg cgaggctacg gcttaaataa
     4081 tcgcgcaatg gcttgttctc ccgcaggatt cgcaatgcga tttctgcgtg accaggtaaa
     4141 tagccattgc cgatgatcat tctgaccggt tttcccacac cctctgcgcc aagcgcggcg
     4201 cggctaaatt cagttgccat actgaaaaag aatatctctc catcatcgcg ggtcgagagg
     4261 attgtcgcca tttctgtacc agggagactg gcgcaattaa agctgacatc ggctagcttt
     4321 tcgtttgtta tattattaat ttggctgaat acatccaatg cgcgggtcgc gtccaggttc
     4381 aaagcctcat cagcgaggcc gagctccagg gctgcctggc agcgatcggc attcttttct
     4441 accaggatta cttgacccgc tgatccagca tgctttttaa tttcatgcag gcacaacatc
     4501 ccagcttttc caccaccaat taccagaatt cgctgaccag cgcgtacatg tcgagcagca
     4561 taagctggag cgccggcgac atccaaagcg atcatggcga gcgggacagg taaatcgctc
     4621 ggaagtatag cccatagtcc ggattcaaac aagatggcat agccttgaac ttcaacctgc
     4681 ccgctagcgg ggtgcacatc atacacttgg tcaagacgca aaggagtgag agccagcgaa
     4741 accatagtga caattgggtc acccactttc agggcaatat ttaaggctgg accaacaaaa
     4801 tcgaccacgc cctttaggac cccgcccgat ccagtaactg gatcctggaa tttaccacga
     4861 gagctaacga tttccttgat tttgttggcc aaattgtttg catcttcata tgcttcgttc
     4921 cacaagcggt aaaagctggt cgaagtgatg tgtaatgcct gcacggtaat accgatctcg
     4981 ttatcgaggg gtggcgcctg attatccaat cgctgcgcgg cctgcggcaa cgcaccaggc
     5041 gggtaaatca cgcgatgttc tccgtagcgg ttacccacat tcatgctttt tctcgctcaa
     5101 ggttcaagat gcgacgagct tcatttggtg ttgccagcgg gcgaccaagc tcgccagcca
     5161 accggaaaac tcgggcaact agcggagcat tgccctggct cagctcacct cgccggtaat
     5221 agatgttgtc ctccaggccg acgcgaacat gtccacccaa aagaatggcc atggtggtca
     5281 gaggtagttg ggatgcgcca atgccgctcg cgcaccaggt gctgcttggt ggcactgact
     5341 cgaccatgtg catcaggttt ttcagcgttc cggcggcgcc acccgggaca cccagaacga
     5401 agttgaaatg gagcggttcg agtagcagtc cttctttaaa caagcgcagc gcatttgcga
     5461 tcatgccgct gtcgaagatc tccaattctg ggcggatgtt cttctccagc atggcacgtg
     5521 caaactggcg tatcatgggg aggctgttgt agaaaacctc ttcgccaaag ttgaccgtgc
     5581 cggtagtaag actggccatg tctggctgga gagctagagg ttgcaaacgc tcttcggcgc
     5641 tcatgcccac tgctccacca gtagaaactt ggatgatcag atcggggcag cgggaccgca
     5701 cagcctggat gatgctttga taggcctgga tgtcttgggt gggggagcca tcagcggcgc
     5761 gcgcatggag atggaccatt gcagccccag cctgttgggc ttcccaggct tcctcagcga
     5821 tctcgtctgg ggtgaggggc aagaaaggcg tatcctggcg ggacaattcc gccccgacca
     5881 ctgcagcggt gatgatcagc ggctgcacca tttaaccctc ccgccttcgc tgtttgtcaa
     5941 atggcactac acaggtgccc tcagcgatgg caaccagttc gggctcgctt agcacctcag
     6001 cagccgatgg gttttcagtg ctgtaggctg agcagatcgt gcggtggacg agaaaacgca
     6061 tctggcggga cgtgcgccct acaccgacca ggtgggcggt gatttccagg aaatcacctg
     6121 cgtggacggg gcgcaggaat tctacccggt tataggcacg gaagagtcct tcatcaccat
     6181 cgtgtcggat gcatagttcg gtggctgcgt cgccgaatag agccatcagc cgtgcgccat
     6241 ccaccagccc tcccccgtaa tggacatcgt gctccgacat acgcaggcgc aatttgaccg
     6301 tcaaaatagg tgcagccgat attgaggacg tgtctgcggt gtttgacatt tatctttgtt
     6361 tctattgata cccgctcgat tggatcaagc tggatagtct atgtaaactg actttgtgcg
     6421 gctatagaaa tctacagccg tcttgccttg ctcgcggaaa gtgttgctgc tgcttttctt
     6481 gaaaccacca aaaggtgctt ggatggctac tcctcccgta gtctggttga ttttcactac
     6541 acctgcctcg atattttccg caaatacaag ggcgtggcgc aggttgttgg tgacaagacc
     6601 cgccgaaaga ccgaactcga tgcggttggc gtaggaaatc gcctcttcca tcgattcaac
     6661 cggcataatg ccgattacag gtccgaagat ttcatcctga gcaatgcgca tttcggggtt
     6721 gacatcggca aatatggtgg gggatacaaa aaagccaggc gtgctaccct ttattgagcc
     6781 accgaccagc agcggttgcg cgccctcgct ttttccaatg tcaatatatt ccaaatcggt
     6841 ttggagctgg gcttcgtcaa cggctgggcc catatgggcg ccttgcattc cattgccgac
     6901 gacgatggaa cgcgcccgct ccaccagccg gctagaaaat tcgggtagga tgccacgctg
     6961 cacaatgact cgactggtgg cggtgcaagc ctgaccggtg agaccaaagc cacccgctgc
     7021 cgccagattg accgccagat tcaagtcggc atcgtctagc acgaccaggg gattttttcc
     7081 gcccatttcc agctgtgtgc gcgccatgcc aggagctgct gactggtgta tgcgatgccc
     7141 taccgggtaa gagccggtga atgagatgcc gcgcacagtt ggactggtca ctagcacgtc
     7201 gccaacttcc cgccctgatc cggtgatgaa attcaacacg cccttgggca agccggcttc
     7261 gtgcaatatc tccaccaggc gcaatgcgca caatggggcg agggaagccg gtttaaagac
     7321 tacagtgcat ccagctacca gggccgggcc gagcttccag gctggaatcg ccatgggaaa
     7381 attccatggg gtgatgatgc taaccacacc aagcggctct cgacgggtaa aaaggatgga
     7441 tttttctttg tcggcaggta aggtctcccc gctgattcgc cggccttcgg cactgtaata
     7501 gcgaaatatg tctcgcgccc tggctacctc agcccgagat tcggcgaaag ttttgccttc
     7561 ttctcgcgtc atcagctctg ccagttcatc cagtcgctgg tggatgatct cggcagcccg
     7621 agagaggaca tcaccccggg caggaggggg ggtagcagcc catttgggaa aagcggcttc
     7681 agctgcttcg agcgcgtggc gcgccgtttc ctggttgcct tttgggaata gtcccacaac
     7741 ttcatctgta ttcgccgggt tgcgccgctc gaaccagtcg ctgccttcaa ctttttcgcc
     7801 gttgatcagg tgataaaact tttctgtcat agatgatctc gctcaggaat tacgcaatca
     7861 ctttctctag aatcaattct tcaatctcat gatcagccag gttgaggtcc tggcgtaaga
     7921 tctcttggct atgctctcct agggcaggag ggtggcgata gatgcgggct ggtgtcgcag
     7981 aatatttggc tactggtcca agctggggaa cgtcttttcc gtctggtcgt tggattattt
     8041 ggaccatttg gcgagcctgt acctgagggt gttgcataac ggtggggatg tcgttgatcg
     8101 gacccacggg tatgcccatt gcgctgagtt gttcgatcca ggttgcggtg gggcggcttc
     8161 ggaagacttc ctgtaacttt ggtatcaact catcgcgaaa tgtaacccgc cctggattgg
     8221 tctgaaagcg ggcatcatcc cgcaggtcag tccgtccagc gacatcacac agccgttgat
     8281 attgtttatc gtttcctacg ccaagggcta ggt
//
LOCUS       DBNGSX010000068         2429 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_835372, whole genome shotgun sequence.
ACCESSION   DBNGSX010000068 DBNGSX010000000
VERSION     DBNGSX010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_835372"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1176)
                     /gene="pyrB"
                     /locus_tag="AC16CB_02175"
     CDS             complement(184..1176)
                     /gene="pyrB"
                     /locus_tag="AC16CB_02175"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013905494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HIR3341303.1"
                     /translation="MTTFSPPSRVQNPFLPFGDQRKAPFYGKDIISVKQFSRPDLDYI
                     FGVAHEMREMVERIGTFDLLKGKILANLFYEPSTRTSSSFTSAMERLGGSVIPINEVR
                     YSSVSKGESLPDTVRTLECYADVIVLRHPEVGASALAAQYARKPIINAGDGIGEHPTQ
                     ALLDLFTIIEELGQADSLTVTMLGDLKYGRTVHSLARLLSLYNVSLHYVSPDILRMPP
                     EIIDELNEKGIPQYEHSTLDEVVPKTDVLYVTRVQKERFADQAEYESVKGAYVITPDI
                     MSKAKERMIVMHPLPRVGEISIEFDSDPRAAYFRQMEYGLYVRMALLAMVFGKA"
     gene            complement(1186..2250)
                     /locus_tag="AC16CB_02180"
     CDS             complement(1186..2250)
                     /locus_tag="AC16CB_02180"
                     /inference="COORDINATES: protein motif:HMM:NF024294.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3341304.1"
                     /translation="MQLPGLIDPHVHMREPGATHKEDWGSGTSAALAGGFTIVLAMPN
                     TQPPVTDRDSFSMALKAARLKALCDYAQYLGAGPDNVEVLPRMALRSAGLKMYLDQTY
                     GPLRLDDMALWMAHFAQWPRQMPIVAHAEGSTLAAVILLALLYDRPLHLAHVSRRDEI
                     LLIRAAKERGIRITCEVAPHHLFLTHDDLPGGRGEVRPRLATAADRDALWANMDVIDC
                     FATDHAPHTQVEKDSPNPPPGFPGLETALPLLLTAVNEGRLNTDDLLTRMVNNPRRIF
                     NLSEQPDTWIEVDTDDVWEIRAADTFTRCAWTPFEGWKVRGRVRKVTLRGREAFRDGK
                     ILAPAGYGKHIRDENRSLAI"
     gene            2221..>2429
                     /locus_tag="AC16CB_02185"
     CDS             2221..>2429
                     /locus_tag="AC16CB_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341305.1"
                     /translation="MGIDQAWKLHAFLTDKKREPVRLSGHIKKFRPSQLALRGETLIN
                     DGHKSIPFNPILRVLWNLSSSFPFTG"
BASE COUNT          555 a          636 c          679 g          559 t
ORIGIN      
        1 tgggcaggta gatctgaggg atgtgggtca agtcctacac ataacagcga atctatctgc
       61 cgggcgcgag cctctatgac cgagaagaat cccatagctc actcacttat ccagttcctg
      121 atggctgggt ttctctgcat taacggacgc aaaagaatat tcaccaggct atttacctca
      181 tctctaggct ttgccaaaaa ccatagccag caatgccatg cgcacgtaca aaccgtattc
      241 catctggcgg aagtaagctg cgcgcgggtc agaatcaaac tcaatgctga tctcaccgac
      301 tcgtggcagg gggtgcatca cgatcatacg ctcttttgcc ttgctcatga tatctggtgt
      361 aatgacgtac gcacccttta cgctttcata ctcggcctgg tcagcaaaac gttctttttg
      421 tacacgggtg acgtaaagca cgtcggtctt gggtactacc tcatcaagag tggaatgttc
      481 gtattggggg atgcccttct cgttgagttc atcaataatc tcaggaggca tgcgcaggat
      541 atctggtgag acataatgca ggctgacatt gtataaagag agcaggcgcg ccagggagtg
      601 aactgtacga ccgtatttta aatcacccag catagtcact gtcaggctgt ccgcctgtcc
      661 cagctcttcg ataatagtga acaggtcgag tagagcctgg gtggggtgtt caccgatgcc
      721 gtcaccggcg ttgatgattg gtttgcgtgc gtactgagct gccagggctg aagcgccaac
      781 ctcggggtgc cgcagcacaa tcacgtcagc atagcactct aaagtgcgca ctgtatctgg
      841 cagcgattcg cctttggaga ccgacgaata gcgcacttcg ttaatgggga taacgctacc
      901 ccccaggcgt tccatcgctg aggtaaatga cgaagaagtg cgcgtggagg gctcgtagaa
      961 caggttagcg aggatcttgc ctttcaacag atcaaatgtg ccgatgcgct cgaccatctc
     1021 acgcatttca tgcgctacgc cgaagatata atccaggtcg gggcgagaga attgcttgac
     1081 cgagatgata tctttcccat agaaaggagc tttgcgctgg tcaccgaacg gtaaaaatgg
     1141 attttgcact cgggaaggtg ggctaaaagt ggtcataaaa tctccttata ttgctagact
     1201 gcggttttca tcgcgtatat gctttccata tcccgcgggt gccaggattt ttccatctcg
     1261 aaacgcttcg cgcccgcgca aagtcacttt ccgcacccgc ccgcgcacct tccagccttc
     1321 gaagggtgtc caggcgcagc gggtgaaagt gtctgcggcg cgaatttccc aaacatcgtc
     1381 cgtatccact tctatccagg tatccggttg ctcggacaga ttgaaaatgc gacgtgggtt
     1441 gttcaccatg cgggttagca ggtcatcggt gttcaatcgg ccttcgttca ccgcagtaag
     1501 cagcaaaggc aaggcggtct ctaagcccgg aaagccaggg ggtgggttgg ggctgtcttt
     1561 ctccacctgg gtatggggag catggtcagt ggcaaaacaa tcgatgacat ccatgttcgc
     1621 ccacaatgcg tctcgatcag cagcggtcgc cagtcgtggg cgcacttcgc cgcggccgcc
     1681 aggtagatcg tcgtgggtca aaaagagatg gtgtggggct acctcacagg taatacgaat
     1741 gcctcgctcc ttggcagccc gaattaagag tatttcatcc cgccgcgaga catgtgccag
     1801 atgaagcggg cggtcgtata gcagcgccag gaggatcacc gctgccagcg tgctcccttc
     1861 agcgtgggca acaatcggca tctggcgcgg ccactgagcg aagtgtgcca tccaaagcgc
     1921 catatcgtcc aggcgcagcg gcccgtaggt ctggtccagg tacattttca atccagcgga
     1981 gcgcaaggcc attctgggga gaacctccac gttgtctggt cctgctccta ggtattgagc
     2041 gtaatcgcag agagctttga ggcgggctgc cttcagtgcc atagagaagg aatcgcgatc
     2101 cgtcacaggc ggctgggtat tgggcatcgc cagcacgata gtaaatccgc cggccagggc
     2161 ggctgatgtg ccgctacccc agtcctcttt atgggtagcg ccgggctcgc gcatgtgcac
     2221 atggggatcg atcaggcctg gaagctgcat gcatttctca cagacaaaaa aagagagcct
     2281 gtacggctct ctggtcatat aaaaaaattt cgcccatcgc agctggcgct gcggggcgaa
     2341 accctgataa atgacggaca taagagtatt ccattcaacc cgattttaag ggtattgtgg
     2401 aatctgtcaa gtagttttcc ttttactgg
//
LOCUS       DBNGSX010000069         2115 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1320270, whole genome shotgun sequence.
ACCESSION   DBNGSX010000069 DBNGSX010000000
VERSION     DBNGSX010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1320270"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="AC16CB_02190"
     CDS             <1..652
                     /locus_tag="AC16CB_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR3341306.1"
                     /translation="MGSTLARPGAPILLALPTVALLNLIDPDSALGTWENGGWNYIIY
                     ITFFLGGFIIFSHEGLQRRIQGQRWLWLVAGVVMLAVAFALWDGKDPLFGSLQFFMIF
                     SLYGLCSWSFILAILGFGMKHLTFSTPFLKYANEAVLPFYVMHQTVLLVIGYFIIQWN
                     IPDLVKFLIIAPTSFGIIVGLYEYLVRRVDILRLLFGMKPQARLQVSVSREKVLAR"
     gene            1075..>2115
                     /locus_tag="AC16CB_02195"
     CDS             1075..>2115
                     /locus_tag="AC16CB_02195"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3341307.1"
                     /translation="MKTIIKSGTLITASDKFTADIFIDGEKITQIGEDLKDESAKIVD
                     ARGKLVMPGGIDPHTHFDLPMFNTVSSDDHYTGHKAAAFGGTTTVMDFVPQERATLKE
                     NVELWRAKADPKAAIDYSFHMNITHFSEAIAEQITSLVDLGITSVKVFTAYNNRLRLM
                     DGDIFRVMRIAKENGFLTMLHAENGDVIEVLVEEALAAGHTSPEWHALTRPTWGAVES
                     ALRGIALTAETGAPLYIVHMNAAGEVDQLSYGRERGLPVMGETCPAYLFFTIDHLRRP
                     DGAKWICSPPMRSEQDNARLWEGIADGTIQTIGTDHCPFFYDGTKPILYENKPIAIPG
                     KELGKDDFTKIPN"
BASE COUNT          521 a          504 c          556 g          534 t
ORIGIN      
        1 catgggtagc accctggcgc ggccaggcgc cccaatcctc ctggctttgc cgaccgtggc
       61 gttgctgaac ctgatcgatc cagactcagc tttaggcacc tgggagaacg gcggctggaa
      121 ctacatcatc tatatcactt ttttcctggg tggtttcatt atcttctccc acgagggatt
      181 gcaacggcgc atccaagggc agcgctggct gtggttggtg gctggtgtgg taatgctcgc
      241 ggtcgccttc gccctttggg atggaaaaga cccgcttttt ggcagcctgc agttcttcat
      301 gatcttctct ctttacgggt tatgctcttg gtccttcatc ctggcgatcc tgggtttcgg
      361 gatgaagcac ctgaccttca gcacgccgtt tctgaaatat gccaatgaag cagtgctacc
      421 cttctatgtg atgcaccaga ccgtgctgct ggtcatagga tacttcatca ttcagtggaa
      481 tatcccagac ctggttaaat tcctgatcat cgcccccact tcgttcggga tcatcgtggg
      541 cttgtacgag tacctggtcc gaagggtaga catcttgcgc ctcttgttcg gtatgaagcc
      601 gcaagctaga cttcaggttt ccgtgtccag ggagaaggtc ctggcgaggt aaattagaag
      661 gtgtaggtcg ccgcactggg tctttttccc gcaggcggcg acctttgtgt ttaaaccccc
      721 gttcccccag cttaactcgt caggtagaaa attgatgact ggttctgatt attcgatcgt
      781 agactaacgt ctgttttcat aggaaaccag gccaaactgc gggaaacctc ttgcaagtgt
      841 tttcgtaaat ccgtggctat aatgtctcta gaaaaatccg acttcagttg agaacatata
      901 aatcgaggta tagatttgat aactcggttt gtggtaaact cacggctatg gattcatagt
      961 cgaggtctaa gatttcggtg tcgattgaaa aaatgagctt gattgctgcg atgtctctta
     1021 cgcaccaata tagaataaat ccatacctgc catgcataag atcgaaagaa aaagatgaaa
     1081 acaataatta aatctggcac cctgattacg gcttcagata agttcacggc ggatatattt
     1141 attgatgggg aaaaaatcac tcaaattggc gaagatctaa aagatgaatc ggcaaagatt
     1201 gttgatgcac gcggcaagct tgtaatgccg ggcgggatcg acccgcatac gcactttgac
     1261 ctgccgatgt ttaatactgt ctcatcagat gaccactaca caggtcacaa ggcagcagca
     1321 tttggcggaa ccacaactgt gatggatttt gtgccacagg agagagccac gctgaaggag
     1381 aatgtggagc tttggcgcgc caaagctgac cctaaagctg ccatcgatta tagcttccac
     1441 atgaatatta cccattttag cgaagcgatt gctgagcaaa taacgagtct ggttgacctg
     1501 ggtattacga gcgtgaaggt atttaccgcc tacaacaacc gcttgcggct aatggatggg
     1561 gacatattcc gtgtgatgcg catagccaaa gagaatgggt tcttgacgat gctgcatgcg
     1621 gagaatggcg atgtgataga agtgttggtt gaagaggctc tagcggcagg gcacacctca
     1681 cccgagtggc acgcgctcac gcgtcctacc tggggagcag tagaatctgc cctgcgcggg
     1741 atcgcattga ctgccgagac cggcgcaccc ctttacattg ttcacatgaa tgctgccgga
     1801 gaagtagacc agctgagtta cgggcgggaa cgaggtcttc cagtgatggg ggagacatgc
     1861 ccggcatatt tgttctttac catcgatcat ctgcgacgac cagacggagc aaagtggatt
     1921 tgctcgccac ctatgcgctc ggaacaagat aacgctcgct tgtgggaggg catcgccgat
     1981 ggtactatcc aaactatagg gacagaccat tgcccgttct tctacgatgg gacaaaacct
     2041 atactttatg aaaataagcc gattgcaatt cccggtaagg agttgggcaa ggatgatttc
     2101 acgaagatac ccaat
//
LOCUS       DBNGSX010000070         2965 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_489408, whole genome shotgun sequence.
ACCESSION   DBNGSX010000070 DBNGSX010000000
VERSION     DBNGSX010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_489408"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1449)
                     /locus_tag="AC16CB_02200"
     CDS             complement(<1..1449)
                     /locus_tag="AC16CB_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341308.1"
                     /translation="MSIILDYVFSGFGLAYKQGISKGQLRSNVKYGLWCYTYNPMNPT
                     FSNDLPIRRIMQTWWPLAASWLMMSLEGPALSAVVARLSQPEINLAAYGGVVFPLALI
                     IEAPIIMMLAASTALSKDWDSYWRLRRYMMFAGLSLTVLHLLVAFTPLYYVVVRDLIG
                     VPSEILEPARIGLMIMTPWTFSIAYRRFNQGVLIRFGRSQAVGIGTVVRLSTNIGVLI
                     VGFALKTVPGIVVGACAASAGVISEAAYAGIAVRPVIRGSLKLAPPVAQALDLRNFLD
                     FYIPLAMTSLLLLVAQPMGSAAISRLPMALNSLAIWPVVSGLIFIFRSFGIAYNEVVV
                     ALLDEPRSYTNLRRFTHWMAAILSLLFLAVAATPLAHFWFESISALPSELVALGQQSL
                     WFALPLPALAVWQSWLQGAILHGKHTGAISEAVIIYMSSMAVLLFASVAWGQMVGINA
                     ALLAMGVSMALQTAWLWWRSRSMLRDVANRDAA"
     gene            1530..>2965
                     /locus_tag="AC16CB_02205"
     CDS             1530..>2965
                     /locus_tag="AC16CB_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3341309.1"
                     /translation="MNLSQPNWQKITRLVLLSRELDKLEVDQLAPQGKVKYQFSSGGH
                     ELAQIMLAQALSHPHDAAAVYYRSRPFMLACGLSVAEALAAGMGRKSILHQGRDAGVV
                     FNMPRREGLTILPSSGDVGAQYTPAAGWAQAISYRKIVLGETEWDGAISAALGGDGSV
                     ASNGFWAALNIATTQQLPLLFFIEDNAYGLSVPTHLQTPGGNIAVNLSGFINLKVLDG
                     DGSDPDDAWEKIRQGVEHVRSGSGPCLLRMSVPRLAGHTFVDDQAYKSPETRLSEAER
                     DPLPRLKQHLDLQSSTWDQILKDVQTELSQALEIAESQPEPTSNEILTHRFYQGVAPA
                     QGGLRPEGELIPLGSDVPQPSGPRINLLDAVRRTLEHEMRINPRMSVFGEDVGVKGGV
                     HGATMDMQKRFGVQRVFDTSLSEDGIIGRAVGIALAGLLPVPEIQFRKYAEPAQEQLS
                     DLGTIRWRTAGKFAAPVVVRIPVGYGKKV"
BASE COUNT          759 a          826 c          756 g          624 t
ORIGIN      
        1 tgcggcgtcg cggttagcca cgtctcgcaa catagaccgg cttcgccacc aaagccatgc
       61 ggtctgcaga gccatgctga cccccattgc caacaaggct gcgtttatgc ctaccatctg
      121 cccccaggca acgctcgcga agagtaaaac tgccatggat gacatataga tgatcaccgc
      181 ttcagagata gccccggtat gctttccgtg caatatggcg ccctgcaacc agctctgcca
      241 taccgcaagg gcgggcaggg gaagagcaaa ccataagctt tgctgtccta atgccaccag
      301 ttcagagggc agggcagaga tactctcgaa ccagaaatgt gccagtggtg tcgcagccac
      361 agccagaaac aacaggctca aaattgccgc catccaatgc gtaaagcgac gcaggttggt
      421 atacgagcga ggttcgtcca acaaagccac caccacttca ttataggcaa taccaaagct
      481 gcggaaaata aatatcaagc ccgaaaccac cggccaaatc gccagcgagt tcaatgccat
      541 tggcaggcga ctaatagcag cactacccat aggctgggcg accaggagca ggagcgacgt
      601 catcgccagg gggatataaa aatcaagaaa gttgcgcagg tcaagcgctt gcgctacagg
      661 gggtgccagc ttcaacgatc ctcttatcac cggtcgcacc gcaataccag cataggcagc
      721 ctcgctaatc acgcccgcgc ttgcagcaca tgctccaacc acgataccag gaacagtttt
      781 taaagcaaaa ccaactatca atacgccgat attggtgctc aatcgcacaa ctgtacctat
      841 tccaactgcc tgggagcgac caaagcggat taaaacaccc tggttaaaac gccgataagc
      901 gatcgagaat gtccaagggg tcataatcat caatccgata cgggctggtt ctaggatctc
      961 acttggcaca ccgatcagat ccctgacgac gacataataa agaggtgtaa aggctactaa
     1021 aaggtgcaaa actgtcaatg agaggccggc aaacatcata taccggcgca aacgccaata
     1081 cgaatcccaa tctttgctca gagcagttga agccgccagc atcatgatga taggcgcctc
     1141 aatgatcaat gccaggggga agaccacccc accgtatgcc gccaaattga tctcgggctg
     1201 cgaaagccgc gccacaactg cgctcagcgc tggtccttcc aggctcatca tcagccaact
     1261 ggcagccaac ggccaccaag tttgcatgat gcgccgaatg ggtagatcat ttgaaaaagt
     1321 cggattcatc gggttgtaag tatagcacca cagcccatac tttacattag atctgagctg
     1381 tccttttgaa atcccctgtt tgtaagccaa gccaaagccc gagaaaacat aatccagtat
     1441 aatactcaaa taatttcacc tatatatatc cacttacggc tcattaagat ttgtattggt
     1501 aagcaggtaa atggaggact tgctatgtca tgaacttatc ccaacccaac tggcaaaaaa
     1561 ttacacgctt ggtgctgctt tctcgcgagc ttgataagct ggaagtggat cagttagccc
     1621 ctcaaggtaa agtcaagtac cagttttcat ctggtggtca cgagctggct caaattatgc
     1681 tcgcacaggc gctatcccac cctcacgacg cagcagccgt ctattaccgc tcgagaccgt
     1741 ttatgctcgc ttgcgggctt agtgtcgccg aggcattagc cgctggcatg ggtcgtaaga
     1801 gtattctcca ccaagggcgg gatgccggcg tcgtttttaa tatgcctcgc cgagaaggac
     1861 tgactatcct gccctcctcg ggtgatgtgg gtgcacaata cacgcctgcc gcaggctggg
     1921 cacaggcaat cagctaccgc aagattgtat tgggtgaaac agagtgggat ggtgccatca
     1981 gcgctgcgct cggaggcgat ggcagcgtgg catccaatgg tttctgggct gcactcaata
     2041 ttgctactac ccaacagctt ccattactgt ttttcatcga agacaatgct tatggcctct
     2101 cggtacccac acatctgcag acgcctggcg gtaatatcgc agttaaccta tctggcttta
     2161 tcaatctgaa ggtattggat ggcgatggca gtgatcccga cgatgcgtgg gagaaaatca
     2221 ggcaaggagt ggagcatgtt cgttctggct ccggcccatg cctgctccgc atgagcgttc
     2281 cccgcttggc tgggcatacc ttcgtagacg atcaggctta caaaagcccc gagactcgtc
     2341 tctctgaagc agagcgggat cccttaccgc gcttaaaaca gcaccttgac ctgcaatcct
     2401 caacttggga tcaaatcctc aaggatgttc aaacggaatt atctcaagcc ctggaaatcg
     2461 ctgaatccca gcccgaaccc acatccaatg aaattctcac ccaccgcttc taccagggtg
     2521 ttgcccctgc ccagggaggg ctgcgacctg aaggagagct catccctctg ggatctgacg
     2581 taccccagcc gtctggccca cgtatcaacc tgctcgacgc ggtgcgtcgc acgctggagc
     2641 acgagatgag gataaatcca cgcatgtcgg tcttcgggga ggatgttggc gtgaagggag
     2701 gtgtgcatgg cgccaccatg gatatgcaga agcgctttgg cgtgcagcgt gtcttcgata
     2761 catccctatc cgaagatggc atcattgggc gtgcggtggg catagccttg gcgggtctgc
     2821 ttcctgttcc tgaaattcaa tttcgcaagt acgcggagcc cgcccaggaa cagctatccg
     2881 atcttggcac aatccgctgg cgaacagcgg gcaagttcgc tgcgcctgtg gtggtgcgaa
     2941 ttccagtggg ttatgggaaa aaggt
//
LOCUS       DBNGSX010000071         3555 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_489609, whole genome shotgun sequence.
ACCESSION   DBNGSX010000071 DBNGSX010000000
VERSION     DBNGSX010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_489609"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="AC16CB_02210"
     CDS             <1..557
                     /locus_tag="AC16CB_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3341310.1"
                     /translation="GGPRGFMGQPDWANLPLIFVITMLCVLIINNFVRSTLGKALNAV
                     RDDEIAAEAMSVDTRKTKMVAFLFSAFWAGVAGGLLAHVLRYVNPSTFGIFKLSEVLA
                     MVYLGGLNSVVGSVVGAVGFNILSEALRPLEILKWIVIAVLLILVMIFRPTGLIAFRE
                     LKIEDLIKPKTETEEEVEHVAASS"
     gene            538..1308
                     /locus_tag="AC16CB_02215"
     CDS             538..1308
                     /locus_tag="AC16CB_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3341311.1"
                     /translation="MSLLQVDNLTVYFGGLRAVHNFHVEIERGQVRGLIGPNGAGKTT
                     IFNLITGIFRPSQGQVVFDGRDIVGLRPHQLAALGIGRTFQNLRLWRHMTALEHVKLA
                     RYSKIHYGLMGAFFGTPQRQREESEIDEMAYNLLDMLGIGRLADQVVTSLPYGVQRRV
                     EIARALAMEPKLLLLDEPTAGMNPEELVEMVNFIRRIRNQFDIAIFLIEHRMKVVMEL
                     CETIQTLDFGEVIAEGTPEEIHSNPRVIDAYLGKEVVV"
     gene            1308..2021
                     /locus_tag="AC16CB_02220"
     CDS             1308..2021
                     /locus_tag="AC16CB_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3341312.1"
                     /translation="MLLSVENLRVSYGGIRALHGIDFSVDEGEIVCIIGANGAGKSTT
                     LRAISRMIQPESGSKLVFKDIDLLKYPADKIVSELGITHVPEGRRLFGNLTVMENLTL
                     AAFSRKNGSGIEQDYERVFSLFPRLAERKTQKAETLSGGEQQMLAVGRALMSGRKMML
                     LDEPSMGLAPVLMMTMFDALREINKEGTTILLVEQNARLALQFAQRGYVLENGHLVLQ
                     GNSKELITNPDVKKAYLGG"
     gene            2192..2842
                     /locus_tag="AC16CB_02225"
     CDS             2192..2842
                     /locus_tag="AC16CB_02225"
                     /inference="COORDINATES: protein motif:HMM:NF015722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HIR3341313.1"
                     /translation="MVKVPAGTFFMGMNQMKSEDNRPEHEIWLDSFLIDIYEVTNKQY
                     RGCVAAGVCNEPEDLRFFADEHFADHPVVYITWYDASDYCQWQKKRLPTEAEWEKAAR
                     GDKGNLYPWGNRLYLDKLNANNQYLGTTPVGNFPAGASPYGAFDMAGNVWEWVEDWYE
                     AYPGSNFQSDLFGHKYKVVRGGSWNHPAEDARSTQRDMAHPERAIRVVGFRCASTR"
     gene            2865..>3555
                     /gene="alr"
                     /locus_tag="AC16CB_02230"
     CDS             2865..>3555
                     /gene="alr"
                     /locus_tag="AC16CB_02230"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR3341314.1"
                     /translation="MNQGILSWAEIDLNAITFNVKALQRHVGRAVEIIAVVKANAYGH
                     GAVAVARTTLEAGATRLAVHRAIEGIELRQGGITAPILIMGYTPPDGAELIARWKLTP
                     SLTTSESALALSAQAISLGASIPVHIKVDTGMSRYGLMPSELVDFLQELNNFPGLQIE
                     GLFTHFATADWADTNFTLQQLQVFNDVLRAAQGSGLHIPIVHAANSAATLNLPQAHFN
                     AVRPGIALYGLQ"
BASE COUNT          944 a          808 c          906 g          897 t
ORIGIN      
        1 taggaggacc gcgcgggttc atgggacaac cagattgggc taatttgcca ttgattttcg
       61 tgatcacgat gctatgcgtg ttgattatca ataattttgt ccgttctacc ttgggaaaag
      121 cgctaaatgc agtgcgggat gacgagatcg cggccgaggc tatgtcagtc gatacccgga
      181 aaaccaagat ggtggctttc cttttttcag cattttgggc gggagtcgcc ggtgggcttc
      241 tggctcatgt cctgagatat gtaaatccat ctaccttcgg tatattcaaa ctaagcgagg
      301 ttttggccat ggtctacctg ggaggcttga actctgtggt cggatcggtc gtgggcgctg
      361 taggtttcaa cattctgtca gaggcattgc gtcctctaga aatcttgaaa tggatcgtga
      421 tcgcggtttt attgatattg gttatgattt tccgtcccac cggactgatc gcttttaggg
      481 aactcaaaat cgaagatctc atcaaaccca aaacggaaac cgaagaagag gtggagcatg
      541 tcgctgcttc aagttgacaa tctcaccgtt tatttcggcg gcttgagagc tgttcacaat
      601 ttccatgtgg agatagaacg tggccaggtc aggggtttaa tcggccccaa cggggctggt
      661 aaaacaacga tttttaatct gatcaccggt atctttcgac cgagccaggg gcaggttgtc
      721 tttgatggca gggatattgt aggtttgcgt cctcaccagc tagcggcttt gggcatcggc
      781 aggacctttc agaatctgcg cctgtggagg cacatgactg ccctggaaca cgtcaaatta
      841 gctcgttatt caaaaatcca ttacgggcta atgggagcat tcttcggcac accccaacgg
      901 caacgtgaag agtctgagat cgatgagatg gcttacaacc tcctggatat gttaggaatt
      961 ggccgtctgg ccgatcaagt cgtgaccagc ctgccctatg gagtacagag aagggtagaa
     1021 attgccagag cattggcgat ggaacctaag ctattgcttc tggatgaacc tactgctggg
     1081 atgaacccag aagagctggt cgaaatggta aacttcatca ggagaattcg caatcagttc
     1141 gacatagcta ttttccttat tgaacacagg atgaaagtag tcatggaact ttgtgagact
     1201 atccaaactc tcgattttgg agaggttatt gcagaaggca caccagagga aatccacagc
     1261 aaccccaggg tgatcgacgc ctatttgggc aaggaggtag tggtctaatg ctactctcgg
     1321 tggaaaattt acgtgtctca tatggtggta tacgagccct tcatgggata gattttagcg
     1381 ttgatgaagg ggaaattgtt tgtattattg gtgccaatgg ggctggcaag agcacaaccc
     1441 tacgggcaat ctcccgcatg atacagccag aatcaggcag taagctggtt ttcaaagaca
     1501 ttgatttgct caaataccct gccgataaga tagtcagcga attggggatt acccatgtcc
     1561 ccgaaggcag gcgactattt ggtaatctga cggtcatgga gaatttaacc cttgcagcct
     1621 tttcacgtaa aaacgggtct ggtattgaac aagattatga gcgcgtgttt tcgctctttc
     1681 cccgtctagc agagcggaaa acacaaaagg ctgagacatt gagcggtggc gaacaacaaa
     1741 tgttagcagt cgggcgagca ttgatgagcg gcaggaagat gatgctgctg gacgagcctt
     1801 ctatgggact agctcccgtc ctgatgatga ccatgtttga tgcattgcga gaaataaata
     1861 aggaaggtac aaccattctc cttgtcgagc aaaacgctcg tctagcactt caatttgctc
     1921 aacgtggata tgtcttggaa aatggtcatt tagtgctcca gggcaactca aaagagctca
     1981 taacaaaccc agatgtgaag aaagcatatc tgggaggctg aaccaaccaa gacttcccta
     2041 aatattgttc ttaataagcg acttattcaa tcctgaggcg tacaaaaaga tagcgacacc
     2101 tcgtgtaacc ttgtttctat tactctcaat acattctttc gtattgaatg catgtctgct
     2161 cacataccgc ccggcacaaa atcacacaaa tatggtcaaa gtccctgccg ggacattttt
     2221 catgggcatg aatcagatga aatcagaaga taacaggcca gagcatgaaa tctggctaga
     2281 ctcattcttg atagatattt acgaagtaac caataaacag tatcgaggtt gtgtcgctgc
     2341 aggggtatgt aatgagcctg aggatttacg cttttttgct gatgagcact ttgccgatca
     2401 tccggttgta tatataacat ggtatgatgc cagtgactac tgccagtggc aaaaaaagcg
     2461 cttaccaact gaagcggaat gggagaaggc agctcgcgga gataagggca atttatatcc
     2521 ttggggaaat agactctatc tggataaact caatgccaac aatcaatatt tgggcacaac
     2581 cccagtgggt aatttccctg caggtgccag cccttatggc gcattcgata tggcgggcaa
     2641 tgtatgggag tgggtagaag actggtatga agcttaccct ggaagcaact tccagagtga
     2701 ccttttcggt cataaataca aggttgtccg gggaggatcc tggaaccatc ctgcagaaga
     2761 tgcacgttcc acacagcgcg atatggctca tcctgaacgc gcaatccgtg tggttggttt
     2821 ccggtgtgca tcaacgcgct aaataggagc agaaaattat atcgttgaat caaggcatac
     2881 ttagttgggc tgaaatcgat ctgaacgcca tcacctttaa tgtgaaagcg ctgcaaaggc
     2941 atgtgggtcg agcggtcgag atcatcgctg ttgtcaaggc aaatgcatat ggtcatggcg
     3001 cggtagctgt tgctcgtacc acgttagaag cgggggcaac cagattagca gttcatcgcg
     3061 ccatcgaagg gatcgaattg cgccaggggg gcataacagc cccaattttg atcatgggtt
     3121 acaccccgcc agacggtgct gagttaatcg cccgctggaa gctcactccc agcctgacaa
     3181 cctcagaatc tgccctggca ctctcggctc aagctatctc cctaggggca tcaatccctg
     3241 tccacatcaa ggtcgataca ggcatgagcc gttatggatt aatgccttca gagctagtag
     3301 atttcttaca agaactgaac aattttccag gtctccaaat cgaaggtctt ttcactcact
     3361 ttgccaccgc cgattgggcg gacacaaatt tcacactgca acagctacag gttttcaatg
     3421 atgtcttgcg cgccgctcaa ggaagcggat tacacattcc aatcgtccac gccgccaaca
     3481 gcgcagcgac attaaacctt cctcaagctc acttcaatgc cgtgcgccca gggatcgctc
     3541 tctatggatt acaac
//
LOCUS       DBNGSX010000072         3951 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1182328, whole genome shotgun sequence.
ACCESSION   DBNGSX010000072 DBNGSX010000000
VERSION     DBNGSX010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1182328"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="AC16CB_02235"
     CDS             complement(<1..586)
                     /locus_tag="AC16CB_02235"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR3341315.1"
                     /translation="MNAIHCINCHLQYPDTDIPYRCPRCGGLFDFEALWSFDPSQVDS
                     GQPGIWRYRHTFGFPDDAPTVSLGEGNTPLLWAQAFGKKVAFKCEYLNPSGSFKDRGS
                     VPLVSFIRSRGVESAVEDSSGNAGASFAAYAARAGLKAQVYIPDSASGAKRRQIEAYG
                     AEVVRIMGPRSNAAEAVRRVADQGAVYASHAYLPF"
     gene            complement(597..1319)
                     /locus_tag="AC16CB_02240"
     CDS             complement(597..1319)
                     /locus_tag="AC16CB_02240"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014448.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030366 - molybdopterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein"
                     /protein_id="HIR3341316.1"
                     /translation="MNRIKVLFFATLRQRAGLQHIFIEVKENTSIQDLKSLLGRQIPA
                     LEPVLQTALASINREFAFTEDLIPLDAEVAFFPPVSGGVELPTIYAITEDQLDLDNVI
                     AQITLPTTGAACIFTGMVRAVTRRLDPHDTIFLEYEAYKPMAEAKMRQVAEEIRSRWP
                     DVEGIAIIQRIGRLLPGTPTVMIACTAAHRDTGVFEAARYGIDRLKEIVPVWKKEVGP
                     AGEVWVEGEYTPERDDTWDSQG"
     gene            complement(1312..1794)
                     /gene="moaC"
                     /locus_tag="AC16CB_02245"
     CDS             complement(1312..1794)
                     /gene="moaC"
                     /locus_tag="AC16CB_02245"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HIR3341317.1"
                     /translation="MNSSLSHIDETGRARMVDVGHKPDTERIAVARGEVHMRAETLAL
                     IRGGALKKGDALSVAQVAGVMAAKRTSELIPLCHPLLLNQILVEFEMDDELPGVRIKA
                     TVRTVGKTGVEMEALTAVSVAALTLYDMAKSAEKTMRIQNIRLIEKHGGQSGDVVQDE
                     "
     gene            1870..2778
                     /gene="ftsY"
                     /locus_tag="AC16CB_02250"
     CDS             1870..2778
                     /gene="ftsY"
                     /locus_tag="AC16CB_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR3341318.1"
                     /translation="MSNVFTKWKEGLSKTSKATFGQIASLLGASEITDQTFEELEALM
                     IQADLGVDTSEEILAALWERERREGMTKSDQLREAFREELRKRLSAPPSIQYPAQPTV
                     ILIVGVNGSGKTTTIAKLGKRFTDQGKRVVLGAADTYRAAAVDQLQIWAERLNLPIIT
                     GQMGGDAGAVAYDAVQAAIARKADIVLIDTAGRLHTRFNLMEELKKVHRVVGKAMPGA
                     PHAVWLVMDATTGQNALQQARAFKDVVQVTGILLAKLDSSARGGMAFAIQRELGLPIL
                     FAGLGEKPTDLEPFDPDAFIAGILAE"
     gene            complement(2825..3316)
                     /locus_tag="AC16CB_02255"
     CDS             complement(2825..3316)
                     /locus_tag="AC16CB_02255"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020533409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIR3341319.1"
                     /translation="MIISIIVAMDEERGIGVNNRLPWHLSADLKRFKALTMGHHILMG
                     RKTYESIGRALPGRVMMVITRQQDYRPEGCLVVHSLQQGLALANSKGESEFFIIGGGE
                     IFAQSLDYADRIYLTLVHTVAGCDVFFPEINKNTWQERDVSDQVADAQNDFPHTFKTL
                     FRR"
     gene            complement(3313..>3951)
                     /locus_tag="AC16CB_02260"
     CDS             complement(3313..>3951)
                     /locus_tag="AC16CB_02260"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIR3341320.1"
                     /translation="KSIIYELIWFLNGDTNIKYLNDHGVRIWDEWADEHGELGPVYGA
                     QWRSWNTGDGRSVDQISQVIEQIKTNPNSRRLIVSAWNVAEIENMALPPCHTLFQFYV
                     ANGRLSCQLYQRSADVFLGVPFNIASYALLTMMIAQVCDLEAGDFVHTLGDAHLYLNH
                     LEQARLQLTRQPYPLPQMRINPAVKSIFDFEYADFELLNYQAHPSIKAEISV"
BASE COUNT          987 a         1060 c          965 g          939 t
ORIGIN      
        1 tgaatggcaa gtaggcgtga ctggcgtaca cagcgccttg atccgcaaca cgacgaacag
       61 cctcagccgc gttggatcgt ggacccataa tacgtaccac ctcagcgccg taagcttcta
      121 tttgccggcg ttttgcacct gatgcagaat ctgggatata aacttgagct ttcaagccgg
      181 cgcgagcagc ataggctgcg aaagacgccc ctgcgtttcc ggatgaatcc tcaactgccg
      241 attctactcc tcgagagcga atgaagctaa ccaatggaac actaccacgg tctttgaatg
      301 atccagatgg atttaggtat tcacatttaa aggccacttt tttaccaaaa gcttgcgccc
      361 aaagcaatgg cgtgtttcct tctcccaaag acactgtagg cgcgtcatcg ggaaacccaa
      421 atgtatgccg ataccgccaa atccccggtt gccctgaatc cacctgagaa gggtcaaatg
      481 accataatgc ttcgaagtca aacaaaccac cgcagcgcgg gcagcgatag ggtatatctg
      541 tatccggata ttgaagatga caatttatgc aatggatcgc attcatccgc tgtctattac
      601 ccctgcgaat cccaggtatc gtctcgctct ggagtatact caccttccac ccagacctcc
      661 ccagcaggcc ctacttcttt tttccatacc gggacgatct ctttaaggcg atcaattcca
      721 taccgagctg cctcaaatac gccagtatca cgatgtgccg cggtacaagc gatcatcact
      781 gtaggtgtgc caggtagtaa tcgcccaatg cgctggataa tggcgatgcc ttccacatct
      841 ggccagcgag agcggatttc ctcggcaacc tggcgcattt tggcctccgc catcggtttg
      901 taggcttcat attccagaaa aatcgtgtcg tgagggtcga gtcgacgggt aactgcgcgc
      961 accatgcccg tgaaaataca ggcggctccg gtagtgggca aggtgatctg agcaatcaca
     1021 ttgtccaggt ccagctggtc ttccgtaatg gcgtaaatcg tcggaagctc aacccctccg
     1081 ctaacgggtg ggaaaaaggc tacctccgca tctaggggaa tgaggtcttc tgtaaaagca
     1141 aactcccgat taatcgaagc caatgcagtc tgcaaaacag gctccagcgc aggtatctgt
     1201 cggcctagca agcttttcag gtcttgaatg ctcgtatttt ctttcacttc gataaatatg
     1261 tgctgcaagc cagcccgttg cctcaacgtc gcgaaaaaca gcactttgat tctattcatc
     1321 ttggactaca tctccgctct gacccccatg tttctcaata aggcgaatat tttggatgcg
     1381 catggttttc tcggcagatt tcgccatgtc gtagagtgtt aaggcagcga cggatacagc
     1441 agtcagtgct tccatctcta cgccggtttt tcccaccgtg cgaactgtag ctttgattcg
     1501 cacacccggc aactcatcat ccatctcgaa ttccacaagg atttgattta acaagagcgg
     1561 atgacagagg ggaatcagtt ctgaggtgcg ttttgccgcc atcaccccag caacctgcgc
     1621 cacggagagc gcatcgcctt tcttgagagc accgccgcgg ataagcgcca gcgtctcagc
     1681 ccgcatatgt acctcaccac gggctacggc aatacgttcg gtatcaggtt tatggcccac
     1741 atccaccatg cgagcacgac ctgtctcgtc aatatgagag agcgaagaat tcatggcgga
     1801 attatatcgt attctagaaa tctacacttt tgaaacatct ttggaaaatt tcaagctata
     1861 attcagccca tgagcaatgt ttttactaag tggaaggaag ggctatccaa gaccagcaaa
     1921 gccacgtttg gtcagattgc ctcactgcta ggcgcgagcg agattacaga ccaaaccttt
     1981 gaggaattgg aagctctgat gatccaggcc gacctgggag tggataccag cgaagaaatc
     2041 ctggctgctc tgtgggaacg cgagcgccgg gaggggatga ccaaatctga ccagctccga
     2101 gaagccttta gagaggagct ccgtaagcgg ctatccgcac ctccctcaat ccagtaccct
     2161 gcccagccga cggtgatctt aattgtaggg gttaatggtt cggggaagac tacgaccatt
     2221 gccaaactgg gcaaacgctt tactgaccag ggcaagcggg ttgtcttggg agcggcagat
     2281 acatatcgag ccgccgcggt ggatcagcta cagatttggg ctgagcgctt gaatctcccc
     2341 attatcactg gccaaatggg tggtgatgcc ggtgctgtgg catacgacgc tgtacaggca
     2401 gctatcgctc gtaaagcaga cattgtgctt atcgacactg caggtcggct gcatacgcgc
     2461 ttcaatttga tggaagaatt gaagaaggta catcgcgtgg ttggcaaagc catgcccggc
     2521 gcacctcatg ctgtctggct ggtgatggac gccaccacag gtcaaaatgc cctccaacaa
     2581 gcgcgcgcct tcaaagacgt ggtccaggtg actggtatct tgctagctaa gctcgattct
     2641 tcggcgcgag gtggcatggc ttttgcgatt cagcgcgagc tcgggctgcc aatactattt
     2701 gccggtctgg gggagaaacc caccgatctg gaaccattcg acccagatgc atttatagca
     2761 ggtatcttag cagaatgatc tcgacttaca ctcaaccgac acaggcataa caaagcacaa
     2821 attcttatcg tctaaataaa gttttgaatg tatgcgggaa atcgttctgc gcatcagcaa
     2881 cttggtcgga tacatctctt tcctgccaag tatttttatt gatctccgga aaaaacacat
     2941 cacacccagc aactgtatgg accagggtta gatatatcct atctgcgtaa tccagactct
     3001 gggcaaagat ttccccgccg ccgataataa aaaactcact ttcccccttg ctattagcca
     3061 gcgctaaccc ttgttgcagc gaatggacca ccagacagcc ctccgggcgg taatcctgct
     3121 ggcgagtgat caccatcata actctgcctg gtaatgctcg accaatcgac tcataggtct
     3181 tgcgccccat caggatatga tgccccatag tcagtgcttt gaagcgcttc aagtctgcag
     3241 ataaatgcca gggcaagcga ttatttacac caatgccgcg ctcctcatcc atcgctacga
     3301 tgattgagat gatcacaccg agatttccgc tttaatgctt gggtgagcct ggtaattcaa
     3361 caactcaaag tctgcatatt caaagtcaaa tatggactta accgcggggt ttatgcgcat
     3421 ctgcgggagc ggatacggtt gtcgggtcaa ttgcaaacgc gcctgctcga ggtgattcag
     3481 gtacaggtgt gcgtcaccga gtgtgtgtac aaaatctccc gcctccagat cacatacctg
     3541 ggcgatcatc atggtcaaaa gcgcatacga ggcaatatta aaaggcaccc ccaaaaagac
     3601 atccgctgaa cgctggtaga gctggcagga tagccgccca ttggcaacgt agaactggaa
     3661 cagggtatgg caagggggta aggccatatt ttcaatctcc gccacgttcc aggcgctgac
     3721 aatcagccgg cgggagtttg gattggtctt gatttgctcg atcacctggg atatttggtc
     3781 cacgctgcgc ccatcgcccg tgttccacga acgccattgt gcaccataaa caggtcccag
     3841 ctcgccatgc tcatcagccc attcgtccca aattcgcact ccgtgatcat ttagatattt
     3901 tatgtttgta tctccattta aaaaccagat taattcataa atgatggatt t
//
LOCUS       DBNGSX010000073         4566 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_212562, whole genome shotgun sequence.
ACCESSION   DBNGSX010000073 DBNGSX010000000
VERSION     DBNGSX010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_212562"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="AC16CB_02265"
     CDS             <1..764
                     /locus_tag="AC16CB_02265"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIR3341321.1"
                     /translation="FARFTTLGGVRAQNLPGGRVRFMNGASGVIGVQYHGEPKASAFE
                     EMFIDLGAKNRADCPVRVGDVAVFERPFTDLGGRLVAKAMDDRIGVAVLIETLRQLNS
                     SPHEIYFVFSVQEEVGVRGATTAAFGVDPDIGLAVDVTRTGDTPRDKKMEVSLGKGPA
                     VKVRDGGMLSDPRVVDWMVRTAEKARLPYQIEILDGGSTDARAIQVSRAGVPAGCLSI
                     PCRYVHSPSEMVDYSDVQNGVQLMVALLSGPIELG"
     gene            769..2046
                     /locus_tag="AC16CB_02270"
     CDS             769..2046
                     /locus_tag="AC16CB_02270"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR3341322.1"
                     /translation="MVKRSLILVLLWLFVAGCTATPTPVITGVPTVTATPTATQPPAP
                     RITPTPDRQLTLEVWLPPQFDTVTGTVAGDLLQARLDQFATEQADVIVQMRIKPLDGP
                     GGLLDSLTTASAAAPLALPDLVALPRPVLETAALKGLLRPFDDLVNNLEDTDWYDYAL
                     QLSQLQDSHFGQPFAGDALVLVYRKEIVPEPPSTLTYTLHSEGPLIFPAGDPQALYTL
                     ALYQAAGGSILDDEGRPFLEEEPLLEVLSYYHEAALNEFTPFWLTQFQSDDQAWDAFQ
                     TGQANMVVTWASRYLKNKSEDIQVVPIPTLDGKNFTLATGWVWALASPFPERQRMAAE
                     LAIFLSDSEFLAQWTEATGYLPPRPSALSDWNTAGLRAIVEDVSSSAYLYPSADVLPG
                     LAAPLSEATILMLKQQRDPASAAQEAVNRLLGP"
     gene            2106..3350
                     /locus_tag="AC16CB_02275"
     CDS             2106..3350
                     /locus_tag="AC16CB_02275"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HIR3341323.1"
                     /translation="MRSPEEESRLGELSVAEDVPSRIPSQDTPETGSSPATTERNWFL
                     EQWERLSQAGLAESVLRLGTHTLLLALILLVAWAMRQFYRLGETQEAQQDAALAAPLP
                     TPTPTEPPPQLPLFDPQAMEASGIMRLAILDTMIPARPRTVVDKYIVEKGDTIFGIAE
                     KFNLQPETILWGNQFALADNPHNLRPDQELNILPIDGTYYQWQAGDGLNGVSKFFGVK
                     PEDIINYPGNQLNPDEIGDFAHPNIEAGTWLIVPGGRREFVTWSAPAIPRDNPGVAKV
                     LGPGACGSVVDGAVGVGAFIWPSNNHFLSGFDYSPASNHPGVDIDGNTGDAVYAADNG
                     VVVYAGWNNWGYGYVVVLNHGNGWQTLYAHLSAYNVGCGQSVYQGTLIGAIGSTGNSS
                     GSHLHYEMMYQGTKVNPWDYLP"
     gene            complement(3347..3496)
                     /locus_tag="AC16CB_02280"
     CDS             complement(3347..3496)
                     /locus_tag="AC16CB_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341324.1"
                     /translation="MMDEKRDASIPFFDVFLFNRNAHLPTYADLYVRRIATEGAGEGE
                     EGIFV"
     gene            complement(3524..4366)
                     /gene="arsM"
                     /locus_tag="AC16CB_02285"
     CDS             complement(3524..4366)
                     /gene="arsM"
                     /locus_tag="AC16CB_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HIR3341325.1"
                     /translation="MTIEQNTQDIRTFVRERYGKIAEDFQPTSEASCGCAPSDSDCCG
                     GESSSSVRFYDTPDAAALPDEVTGLSLGCGDPVTLAALQPGQTVLDLGSGGGIDCFLA
                     AQRVGADGQVIGIDMTPAMLDKARANQKKLGIENVEFRLGEIEHLPVPDSTVDVIISN
                     CVVNLSPDKPQVFREAFRALKSGGKLAVSDLVTDGPLPQAVKSSLSAWAGCVAGALDV
                     QEYISAIEAAGFVDVKLNPVYFEPQTIDEAIDQVGEAFDLKDIPSESLRKTVFSAKIT
                     AIKP"
BASE COUNT          997 a         1272 c         1218 g         1079 t
ORIGIN      
        1 gttttgctcg ctttaccacg ctcgggggcg tgcgagccca gaatcttcct gggggtaggg
       61 tgcgtttcat gaatggcgcc tcgggcgtga tcggcgtgca atatcacggc gagcccaaag
      121 cctcggcttt cgaagagatg ttcatcgacc tgggcgcaaa aaatcgggca gattgcccgg
      181 tgcgagtggg tgatgtcgct gtctttgagc gcccattcac cgacctgggc ggacgactgg
      241 tagccaaggc gatggacgat cgcatcgggg ttgcagtatt gatcgagaca ctgcgccaat
      301 taaattcatc tccacacgag atctatttcg tcttcagtgt gcaggaggag gttggcgtgc
      361 gtggggctac cacagccgca ttcggtgtag acccagatat tggcctggct gtggatgtaa
      421 cccgcaccgg cgacactcct agggataaaa agatggaggt tagcttggga aaaggcccag
      481 ctgtcaaggt gcgtgatggt ggtatgctca gcgacccacg cgtggtggat tggatggtgc
      541 ggactgccga aaaagcacgg ctgccctacc agatcgaaat attagatgga ggcagcacag
      601 atgcccgtgc tatccaggtc tcaagagcgg gtgttcccgc aggatgccta tccatcccat
      661 gtcgctatgt tcactcgccc tctgaaatgg tcgattacag cgatgttcag aatggtgtcc
      721 aactgatggt cgcgctgctc agcgggccaa ttgaactagg ataggttgat ggtaaaacga
      781 agtttgattc tggttttgct ttggctcttc gtggcgggtt gtactgcgac gcccacgccg
      841 gtaattaccg gcgttcccac tgtgacagct acccccacag ccacgcagcc gcccgcgccc
      901 aggatcaccc ctaccccaga caggcaactg accctggaag tctggttgcc acctcagttt
      961 gacaccgtga caggcacggt cgctggagat ctgctccagg cccggctgga tcaatttgct
     1021 acagaacaag cagatgtgat agtgcaaatg cgcatcaaac ctctagatgg accgggtgga
     1081 ttgctcgatt cgctcaccac cgccagcgca gctgcgccgc tggccttgcc agacctggtc
     1141 gctttgcccc gtcctgtgct ggaaacggct gccctcaagg ggcttctgcg cccatttgac
     1201 gacctggtca ataaccttga ggatactgat tggtatgatt atgccttgca gctgtcccag
     1261 ctccaagata gtcattttgg gcagcccttt gctggcgacg cgttagtcct ggtctaccgc
     1321 aaagaaattg tacctgaacc tccttcaaca ttaacctaca ccttacatag cgaaggacct
     1381 ctaatatttc ctgcaggtga cccgcaggct ttatacactc ttgcgctcta ccaggcggca
     1441 ggcgggtcca tattggatga cgaaggacgt ccctttttgg aagaagaacc gctcctagag
     1501 gtgctgtcct attaccatga ggctgctctc aatgagttca cacctttctg gcttacccag
     1561 tttcagagcg atgatcaggc ctgggatgct ttccaaacag gccaggccaa tatggttgtc
     1621 acctgggctt cgcgttatct gaagaataag tcggaagaca ttcaggttgt ccccataccg
     1681 actctggacg gcaaaaattt caccttggct acaggctggg tttgggcctt ggctagtcct
     1741 ttccctgagc gccagcggat ggctgccgaa ctggcaatat ttcttagtga tagtgagttt
     1801 ctggcacaat ggacggaagc tactggctac ttgccgcctc gccctagtgc attgagtgac
     1861 tggaatactg cgggactgcg tgccatcgtt gaagatgttt cttcgtctgc ctatctctac
     1921 ccttctgcgg atgtactccc tggcttggca gcgccgctgt ccgaggctac catcctgatg
     1981 ctaaaacagc agcgcgatcc cgcctcggcg gctcaagagg ctgtaaatcg tctccttggg
     2041 ccttaatccc acaattttcc tggtttgacg aaaacccctg ccagtgttat tatccgtgca
     2101 caaatatgag atcgcctgaa gaagaatccc gcctcggcga actttctgtc gccgaggatg
     2161 tgccttcccg gataccctct caagatacac cagagacagg ctcctccccc gcaacaacgg
     2221 aaaggaactg gtttctggaa caatgggagc gcttatcgca agcgggtttg gccgagtcag
     2281 tgctccgctt ggggacgcac acattactac tagctttgat actgctcgtt gcatgggcta
     2341 tgcggcaatt ctatcgcctg ggagagacac aagaagccca gcaggatgcc gcgttagccg
     2401 caccgctgcc taccccgacg ccgacagaac cccctcctca gttgccgctc tttgatcccc
     2461 aagcgatgga agcctcgggg ataatgcggc tagcgattct ggatactatg atcccagctc
     2521 ggccgcgcac tgtagtggat aaatacatcg tagagaaggg cgatacaata ttcgggatcg
     2581 ccgaaaaatt caatcttcag cctgagacca tcctatgggg aaatcaattt gccctggctg
     2641 ataaccccca taacctgcgc ccagatcagg agctaaacat cctgccaatt gacggcactt
     2701 attatcaatg gcaagccggc gacgggctaa acggcgtttc caagttcttt ggtgtcaagc
     2761 ccgaagatat aatcaattac cctggtaatc aactgaaccc ggatgaaatc ggtgattttg
     2821 cccaccccaa catagaagcc ggcacatggc tgatcgtacc gggtggtcgg cgcgaattcg
     2881 ttacctggag cgccccagcc atcccgcgcg ataacccagg cgtagccaag gtgcttggtc
     2941 ctggggcttg cggctcagtt gtggatggtg ctgttggtgt gggtgccttt atctggccct
     3001 caaataacca cttcctgtct ggctttgatt actctcctgc ttctaatcat cctggcgttg
     3061 atattgacgg aaacactggc gatgcggtgt acgcagccga taatggcgtt gtcgtctatg
     3121 ctggatggaa taactggggc tacggttatg tcgtggttct caaccatggg aacggctggc
     3181 agacccttta tgctcacctg agcgcctaca atgtaggctg cgggcagagc gtctatcaag
     3241 gaactttgat tggagccatc ggtagcaccg gaaactcctc tggctcgcac ctgcactacg
     3301 agatgatgta ccagggcacc aaggtgaatc cctgggatta cctcccctaa acgaaaatcc
     3361 cttcctcacc ctccccagct ccctcagttg ctatcctcct gacatagagg tctgcatagg
     3421 ttggcaggtg ggcgttcctg ttgaatagga agacatcaaa aaacgggatg ctggcatccc
     3481 gtttttcgtc catcatcccc tccccatctg ggtaatccaa aaattatggt tttatcgctg
     3541 tgatttttgc actgaacacc gtcttgcgca gcgactcact gggaatgtct ttcaggtcaa
     3601 acgcctcgcc gacctggtca atggcttcgt cgatcgtctg cggctcaaag tagaccggat
     3661 tcagcttcac atccacaaag ccggcagctt cgatggcgga gatgtattcc tggacatcta
     3721 aagcgcccgc cacgcagcct gcccaggcgc tgaggctgga cttaactgcc tggggcaggg
     3781 gaccatccgt cactagatcg ctcacggcaa gctttccgcc cgatttgagc gcacggaaag
     3841 cctcgcgaaa tacctgtggc ttgtcgggac tgagattgac cacacagttt gagatgatca
     3901 cgtcaacggt gctatccggt acaggcaggt gctcgatctc gcccaatcgg aattctacat
     3961 tctcaatgcc cagctttttt tggttggcgc gcgccttatc cagcatggcg ggagtcatat
     4021 cgatgccaat tacctggcca tcagcgccca cccgctgcgc agccaggaag caatcgatgc
     4081 caccaccgga gcccaggtcc aatacagttt gaccaggctg tagtgcggcc agcgtcactg
     4141 gatcgccgca acccaaagag agaccggtaa cttcatccgg cagggcagct gcgtcggggg
     4201 tgtcgtagaa gcgcacagag ctactggact ctccgccaca gcaatcgcta tccgatgggg
     4261 cgcagccgca agaggcttct gaggtcggct ggaaatcctc agcgatcttt ccatatcgtt
     4321 ctcgcacgaa ggtgcgaatg tcctgggtgt tttgttcaat ggtcatgata ttctcctaat
     4381 atattgaaat atttatatgt gtctatatgt tacacgcagt ttttactctt gtcaagatta
     4441 aatacgcata attacgccaa aacaccccaa acactctttt tgcgggattg cgcatgccct
     4501 gaggggcata tgcaattgcc ctggcgctga atctggattt ctcctcataa ggagattcag
     4561 gtatca
//
LOCUS       DBNGSX010000074         1743 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_420257, whole genome shotgun sequence.
ACCESSION   DBNGSX010000074 DBNGSX010000000
VERSION     DBNGSX010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_420257"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="AC16CB_02290"
     CDS             complement(<1..638)
                     /locus_tag="AC16CB_02290"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3341326.1"
                     /translation="MDSELLAEMMVAILERGYEIDSVSIIRNGYLVADVSVYPFRPNT
                     KHVIHSCTKSIVSILIGIAIEQGYIEGVQQPILSFFPGKTVANLDARKEAMTLEQVLS
                     MATGLNCRDSYLYRWQGLNQMRESDDWVQFMLDLPMVEAPGTRFEYCNGASYLLSAII
                     KQTTGVSALEFAQEHLFGPLGISDVVWPYSPQEVNIGWGEIRMLPLDMAKIG"
     gene            complement(1031..1264)
                     /locus_tag="AC16CB_02295"
     CDS             complement(1031..1264)
                     /locus_tag="AC16CB_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341327.1"
                     /translation="MEVILTTLASMFKTDERFIMHRYYSTRISMMVGIILIVAWFSYE
                     LLVNNLLRWDLAIIAGAMAITKILAMLYFRATG"
     gene            complement(1255..1479)
                     /locus_tag="AC16CB_02300"
     CDS             complement(1255..1479)
                     /locus_tag="AC16CB_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015321249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3341328.1"
                     /translation="MGTEVRNQIKERRLRHGLSQEELAKAVGVSRQSINSIERGRYIP
                     SLPLALRFARLFDCPLDDLFQLQEEGETWK"
     gene            complement(1550..>1743)
                     /locus_tag="AC16CB_02305"
     CDS             complement(1550..>1743)
                     /locus_tag="AC16CB_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341329.1"
                     /translation="EVELYGSLVHVVAPQMQEQQRLIAAKLRQFGIQAGQMSIIEPSL
                     EDVFIACMKGKAARDPHKT"
BASE COUNT          451 a          464 c          406 g          422 t
ORIGIN      
        1 atcccaatct tcgccatatc cagaggcagc atgcgtattt caccccaccc gatatttacc
       61 tcttgagggc tgtacggcca gaccacgtcc gagatcccca atggcccaaa tagatgctcc
      121 tgagcaaatt caagagcgct caccccggtc gtttgcttaa taatagcgga cagaagatac
      181 gaagctccat tgcagtactc gaaccgcgtt cctggggctt ccaccatagg caggtccagc
      241 ataaattgaa cccaatcgtc actttctctc atctggttca gtccctgcca gcgatagaga
      301 tacgaatcgc ggcaatttag ccccgtcgcc atgctcaaga cctgctccag cgtcattgcc
      361 tctttgcgtg catccagatt tgcaactgtc ttccccggga aaaagctcaa aatgggttgt
      421 tgcactccct cgatataccc ttgctcgatg gcgataccaa ttaatataga gacaatgctc
      481 ttggtacagg aatggatgac gtgcttcgta ttcgggcgaa acgggtaaac cgaaacatct
      541 gccaccaggt agccattccg gatgattgaa acgctgtcaa tttcgtagcc ccgctccagg
      601 attgccacca tcatttctgc taacaattcc gaatccatgc cttgttgctc aggcgtagag
      661 gtccgaaaac ccttggttgg ccagtattcg acaaattcct gtgtcgcaac cggtgccacg
      721 gcgggtgtaa cttgagcgca tccagaaagg aacgcgagga tacaaaacag aagaagagat
      781 ctgctgattt ttaggttaaa gatcatcaca cccccggagt tctagctaat ttttgttaat
      841 gtaaaccagt caatataaca ataatactcc cgacactcgc cgggcgttta aatttccttg
      901 aacccgatgt atggaatatg cagatgaaaa gctgggatca cctggctgta caaccggaat
      961 gatcaaagag tgttgatcac aatgctagtc cacttgccga aggaggctat agccaactgc
     1021 atccatgcaa ttaaccagtg gctcggaaat agagcatcgc cagaattttt gtgatcgcca
     1081 tcgcccccgc aatgatagcc agatcccagc gcagtagatt attgactaaa agctcatagc
     1141 tgaaccaagc cacaatcaga atgatcccga ccatcatgga tattcgagta gagtaatacc
     1201 tgtgcatgat gaagcgctca tccgttttga acatgctggc cagtgtggta agtatcactt
     1261 ccatgtctcg ccttcctcct ggagttggaa caaatcatcc aacggacaat caaataatcg
     1321 ggcaaatctc aacgcgagtg gtaagctggg tatgtaccgc cctcgctcga tggagttgat
     1381 gctctgacga gacacaccga cagccttcgc gagctcttct tgagagagac cgtgccgcaa
     1441 gcggcgttct ttgatttgat tcctgacttc agtgcccatt ctaaattccg agtagatgtc
     1501 aagtctactt gacatcatac acgagaaatt ccgcggtgtc aagttggctt tacgttttgt
     1561 ggggatctcg agctgcttta cccttcatgc aggcgatgaa cacgtcttcc agggaaggct
     1621 cgatgatgga catctgtcca gcctggatgc caaactggcg caatttggca gcgatgaggc
     1681 gctgctgctc ctgcatctgc ggcgcgacca cgtgtacgag tgagccatac aactcgactt
     1741 cgg
//
LOCUS       DBNGSX010000075         5962 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_143465, whole genome shotgun sequence.
ACCESSION   DBNGSX010000075 DBNGSX010000000
VERSION     DBNGSX010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5962)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_143465"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="AC16CB_02310"
     CDS             <1..415
                     /locus_tag="AC16CB_02310"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3341330.1"
                     /translation="VFWSTPIVSWSKLTPDTMLFLFGARPEDASLIPGEVIPESMLRA
                     LKALADPTRLQILRYLSEGNLTPAQLSRRLRLRAPTVIHHLKTLRLAGLVHLTVDREG
                     ESSYAARLEAISGTLSHVGDFLGSEMQSEMELIEV"
     gene            624..1019
                     /locus_tag="AC16CB_02315"
     CDS             624..1019
                     /locus_tag="AC16CB_02315"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR3341331.1"
                     /translation="MEMIVDFPGGARVDAHFGSFTLKTDQPPAASAPTPFATFLASIA
                     TCAGIYVLGFCQQRGLPTDGIRIIQRTSKDPASGMVSKIDLEIQVPPSFPEKYLPSLV
                     RSAELCAVKKHIEKPPVFDVFTKTVEPAA"
     gene            complement(1159..2418)
                     /locus_tag="AC16CB_02320"
     CDS             complement(1159..2418)
                     /locus_tag="AC16CB_02320"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HIR3341332.1"
                     /translation="MPNLNNVLAVILGGGVGKRLHPLTKQRAKPAVPIAGKYRLIDVP
                     ISNCINSGISHIAVLTQFNSVSLHRHITQTYTFDDFHRGWVQILAAEQTPRSTDWYQG
                     TADAVRKQLFEIKATDAELVLILAGDHLYRMDYSSMVEFHYEQGADVTVAVQPVAASE
                     SFRFGLLKKDPDQHIAAFVEKPRDPNVLAQFVSRDDPARPYLGSMGIYVFNTAVLVDL
                     LENSLHEDFGGQIIPNAIHTHKVYGFDFDGYWEDIGTIRSFFETNLSLTQPNPPFNFY
                     DPVRPIYTRQRHLPSTVIDGATLHNVMLAEGSLIHHAEINDSIVGLRSIIADGVRINK
                     SIIMGADYYDEPEVLSRKGIPLGIGRDCRIEGAIVDKNVRMGEGVTILPFERGTDLDA
                     GSWVVQDGIVVIPKSTILHPGTYIGPD"
     gene            complement(2608..2689)
                     /locus_tag="AC16CB_02325"
     tRNA            complement(2608..2689)
                     /locus_tag="AC16CB_02325"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2653..2655),aa:Leu,seq:caa)
     gene            2766..3836
                     /gene="ychF"
                     /locus_tag="AC16CB_02330"
     CDS             2766..3836
                     /gene="ychF"
                     /locus_tag="AC16CB_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR3341333.1"
                     /translation="MKLGIIGLPQAGKTTVFNALTRGNAPTTMSGGRFEVHTAVVDVP
                     DQRVDRLSAIFRPQKTVYAKVTYADIAGLEAGASKAGISGTLLNQLTQMDGFVHVVRC
                     FQEASVPHPSGSVDPQRDIDAMDAELLLNDLIAVERKLERLGDERRKGGGRDKAVIER
                     ELALFEKLHDALGQEKPLREVEFSAEEQKSLSGFGFLTRKPVLLVLNLSDEQTPPDVA
                     YPHAHTAVMPLQGRLEMELAQLSPEDAEVFMGEYGIQELSLYKVIRLSYDLLGLQSFF
                     TIGSDEVHAWTICRGDNAHSAAGVVHTDLQKGFIRAEVISFDDLVALGGLSEARAKGK
                     LRLEGKDYILQDGEIMHVRFNV"
     gene            3909..5687
                     /locus_tag="AC16CB_02335"
     CDS             3909..5687
                     /locus_tag="AC16CB_02335"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIR3341334.1"
                     /translation="MTLPYTQTRWSLSDLFPALDSPELEQTFTDLETQVAKFESMRSQ
                     LSSQIKQAEFMNFVTLLEEINQLGSRLYAFAGLSFSADTQDQIAQTLVARVDQFIAIL
                     QNRTLFFSLWWKDLDDDNAARLMSEAGDYRYWLEEMRHFKPHTLTEPEEKIINIKNVT
                     GVSALGNLYDSITNRYTFKLQVDGEVKELTRGELMVYVRQHDADLRAAAYQELYRVYG
                     EEGPILGQIYQTLVRDWRNEQVDLRRFSSPISARNLMNDIPDPVVNTLLDVCERNASL
                     YQRYFRLKAKWLGMDRLRRYDIYAPVVKSDKRYEYTQAAEMVLGSFHEFEPRLAGLAR
                     RVFAENHLDSEVRKGKRGGAFCMSVTPKMTPYVLLNYQGRADDVATMAHELGHAIHTM
                     LAADHSMFTFHASLPLAETASTFGEMMLIDRLLAQEDDESVRRDLLFRQVDDSYATIM
                     RQAYFALFERDAHEMVDQNASADELSAAYMENLKRQFGEVVEISDEFRWEWVSIPHIY
                     QVPFYVYAYAFGQLLVLSLYKQYKVEGESFKPRYVSLLSSGGSEAPERLLSQAGIDIR
                     SADFWQGGFDVLSGLLDQLEEIPIDA"
BASE COUNT         1326 a         1606 c         1662 g         1368 t
ORIGIN      
        1 tgtattctgg agcacgccaa tcgtttcatg gagtaagctc acccccgata cgatgttgtt
       61 tttgtttgga gcacggccgg aggatgcttc cttgattccc ggggaggtga tacccgaaag
      121 catgttgcgc gccctcaagg cactggctga ccccacacga ttgcagatat tacgctacct
      181 gagcgaagga aacttgacgc ccgcccaact ctcgcgccgg ctgcggttgc gcgccccaac
      241 cgtcatccac catctgaaaa cgctgcgcct ggctgggctg gtgcatttga ccgtggatag
      301 agagggtgaa agcagttatg ctgctcgcct ggaagctatc tctgggacct taagccacgt
      361 gggggatttt ctggggagcg agatgcaatc tgagatggaa ctaatcgaag tataaaaacc
      421 ttatactcct ctcttgtgtg ctactccctc ttcggtctct ggctgagagg gggtagtttc
      481 tacttaaggg gagtttccgt attgtcctga ataaatctct tcccggcacc cttttccttt
      541 tgggcgggta tgtaactttt taagggctat ggcatctaat aaattgtgag ataatacaca
      601 actagtagag gaggtccttt ttcatggaga tgattgttga ttttcccggc ggagcccgcg
      661 tggatgccca ctttggctca ttcactttaa aaacagacca gcccccggca gcttcagccc
      721 ccaccccgtt tgccactttc ctggcctcca tcgcgacgtg cgccgggatc tatgttctcg
      781 gtttttgcca gcagcgcgga ctgcccacag acggtatccg cattatccag cgcacctcaa
      841 aagatccagc gagtggcatg gtgagcaaga ttgatctgga gattcaggtt cccccttctt
      901 tccccgagaa atacctgccc tccctggtgc gttcagcaga gctgtgtgcg gtgaaaaagc
      961 atatcgagaa accaccagtt tttgatgttt ttactaagac cgtcgagccc gcagcttaag
     1021 gaacaccctt gatcccacct aacagttagg tggtcaggca agagattgga aataacgagt
     1081 tgttatgcgg cagtttcgcc gcataacaac tcgttttctt taagcaagga agagatggga
     1141 gcgttgggag aaaatcaact aatccggacc gatgtaggtg cctgggtgca ggatggtgct
     1201 cttggggatg accacgatgc cgtcttgaac tacccaacta cccgcatcca aatctgttcc
     1261 gcgctcgaag ggtaggatag tcaccccttc gcccatgcgc acattcttat ccacaatggc
     1321 tccctcgata cggcagtcac gcccaatccc tagcgggatc cctttgcggg agagcacttc
     1381 cggctcgtcg tagtaatctg cccccatgat gatgctcttg ttgatgcgca ccccatcggc
     1441 gatgatgctg cgcaacccga cgatcgaatc gttgatctcc gcatgatgga tcagactgcc
     1501 ttcagccagc atcacgttgt gtagggtggc gccatcgatg actgtgctgg gcaggtggcg
     1561 ctggcgggtg tagatcggac gcacagggtc gtaaaagtta aagggagggt tgggctgggt
     1621 caggctcagg ttagtctcga agaatgaacg gatcgtcccg atatcctccc aatagccatc
     1681 gaaatcgaag ccatagacct tgtgggtgtg gatggcgttg gggatgatct gaccgccgaa
     1741 gtcttcgtgc agggagtttt ccagcaggtc caccagcacg gcggtgttaa agacgtaaat
     1801 gcccatcgaa cccaggtagg ggcgcgctgg atcgtcgcgg ctgacaaact gtgccagcac
     1861 gttggggtcg cggggtttct ccacgaaagc agcaatgtgt tggtccggat ccttcttgag
     1921 cagaccgaag cggaaagatt cgctcgccgc caccggctgg acagcaaccg ttacatcggc
     1981 tccctgttca taatgaaatt cgaccataga actgtaatcc atgcggtaaa gatggtcgcc
     2041 agccaggatg agcacgagct cggcatcggt ggctttgatt tcgaagagct gcttgcgcac
     2101 ggcgtcggcg gtgccctggt accagtcggt gctgcgcggg gtttgttccg ccgccaggat
     2161 ctgaacccag ccgcgatgga aatcgtcgaa ggtgtaggtc tgtgtaatgt ggcggtgcag
     2221 ggagaccgag ttgaactggg tgagcacagc gatgtggctg atgccggagt tgatgcagtt
     2281 actgataggc acatcgatca ggcggtactt tccggcgatg ggtacggcag gcttggcgcg
     2341 ctgtttggta agcgggtgga gacgcttgcc tacgccgccg cccaggatga ctgctaagac
     2401 attattcaaa tttggcatga caacctctgt gttttactct gaaggggcaa tggaacacgc
     2461 tagcatccaa attcatttct cggattggca catcgtgttt taaatggaac cctgtttaat
     2521 tatagccgca tatcccttaa gcaagcgacg cacgaggcgt ttattgatgc actcatgcgt
     2581 ctgcctgtcc gcgtgctata atgtcagtgc cgaaggtggg aatcgaaccc acacccccga
     2641 ggggacacga ttttgagtcg tgcgcgtctg cctattccgc cacttcggct cgatgacaat
     2701 tatactcaaa ttatctgtaa tcttcaatca gcatcttatc agggtgacct acacctgaaa
     2761 catctatgaa actgggaatt atcggactcc cccaagcagg aaagaccacc gtatttaacg
     2821 ccctgacacg cggcaatgct cctaccacca tgagcggcgg gcgttttgaa gtacacaccg
     2881 ccgtggtgga tgttcccgat caacgcgtcg atcggctatc cgccatcttc cgtccccaaa
     2941 agaccgtcta tgccaaggtc acctatgccg atattgccgg tttggaggcc ggagccagca
     3001 aagcgggtat ctcggggact ttgcttaacc aattgaccca gatggacggc tttgtgcacg
     3061 ttgtccgctg ctttcaggag gctagcgtgc cccacccatc cggcagcgtc gacccacagc
     3121 gtgacatcga cgccatggat gcggagcttc tgctcaacga tctgatcgct gtggagcgca
     3181 agctggagcg cctgggtgac gagcggcgta agggcggggg acgtgataag gcggtgatcg
     3241 agcgcgagtt agcccttttt gaaaagctgc acgacgccct cggacaggag aagcccttgc
     3301 gggaagttga gttctccgcc gaagagcaaa agtctctctc gggttttggc ttcctgaccc
     3361 gcaaaccagt gctgttggtg ctgaacctct cggacgagca gacgccgccg gatgtggctt
     3421 accctcatgc tcatacggca gtcatgccgc tgcaaggacg cttggagatg gagctggcac
     3481 agctctcgcc ggaggacgcc gaggtcttta tgggggagta cggcatccag gaactgagcc
     3541 tgtacaaggt gatccgcctg tcatacgatc tgttgggctt acagtctttc tttaccattg
     3601 gatcagacga ggtgcacgcc tggaccatct gccggggcga caacgcccac agcgcggcgg
     3661 gcgtcgtcca taccgatctg cagaaaggct tcatccgcgc cgaggtgatt tcctttgatg
     3721 atctagttgc gctgggaggg ttaagcgagg cccgcgccaa aggcaagctg cgcctggagg
     3781 gcaaagacta tatccttcag gatggcgaga tcatgcacgt caggttcaat gtgtgagcca
     3841 atgagccctt cgggtataat actctccgcc atcttgacta tatatattca tatcaaagag
     3901 agatccccat gaccttaccc tacacccaga cccgctggag ccttagcgac ctttttccag
     3961 ctctggatag cccggagctg gagcagacct ttaccgacct agaaacccag gttgccaaat
     4021 ttgagtccat gcgctcgcag ttgtcctcac agatcaagca agccgaattt atgaactttg
     4081 tcaccctact ggaagagata aaccagttgg gatcgcgcct gtatgccttt gctggattga
     4141 gcttcagcgc cgatacacag gaccagatcg ctcagaccct ggtagcccgc gtggaccaat
     4201 ttatagcaat cctgcagaac cgcacgctct tcttcagcct gtggtggaag gatctggacg
     4261 acgacaacgc ggcgcggctg atgtctgaag ctggcgatta ccgatactgg ctggaggaga
     4321 tgcgccactt caagccgcat accctcaccg agccagaaga gaagatcatc aatatcaaga
     4381 atgtcactgg tgtaagcgca ctgggcaacc tgtatgactc gatcaccaac cgctatactt
     4441 tcaagctgca ggtggacggc gaagtcaagg aactgacccg cggggagttg atggtatacg
     4501 tccgacaaca cgatgccgac ctgcgtgccg ccgcctacca ggagctctac cgcgtttacg
     4561 gggaagaggg tccaatcttg gggcagatat accagacttt ggtgcgcgac tggcgcaacg
     4621 agcaggtgga cctgcgccgc ttcagcagcc cgatcagcgc ccgcaacctg atgaacgata
     4681 ttcccgatcc ggtagttaat accctgttgg atgtttgtga gcgcaatgca tccctgtacc
     4741 aacgctattt ccgcctcaag gcaaaatggc tgggaatgga tcgcctgcgc cgatacgata
     4801 tctacgcgcc ggtggtcaaa tcggataagc gttacgagta tacccaggcg gctgagatgg
     4861 tgctgggctc attccacgaa tttgagccca ggctggcagg cttggccagg cgcgtcttcg
     4921 ctgaaaatca cctggatagc gaggtgcgaa agggcaagcg cggcggggct ttctgcatgt
     4981 cggtgacgcc caaaatgaca ccttatgtgt tgctcaacta tcagggcaga gcagatgatg
     5041 tcgccaccat ggcgcatgag ttgggacatg ccatccacac catgctggca gccgaccaca
     5101 gcatgttcac attccacgcc tcgctgcccc tagcggaaac ggcttctact tttggcgaga
     5161 tgatgctcat cgatcgcttg ctggcacagg aagatgacga gagcgtccgc cgtgacctgc
     5221 tcttccgcca ggtggacgat tcctacgcca cgatcatgcg ccaggcatac ttcgcactgt
     5281 ttgagcggga tgcccacgag atggtagatc aaaacgcttc ggcggatgag ctctcagcag
     5341 cctatatgga gaacttgaag cgtcagttcg gcgaggtggt cgagatcagc gacgaatttc
     5401 gctgggagtg ggtttccatc ccgcacatct atcaggtacc gttctatgtg tacgcctatg
     5461 cctttggtca gctcctagtg ctatccctgt acaaacagta caaagtggag ggcgaatcct
     5521 tcaagccacg ctatgtcagc ctgctttctt ctggtggttc cgaagcccca gaacgcttgc
     5581 tctctcaggc agggattgat atccgcagtg cggatttctg gcaagggggc ttcgatgtgc
     5641 tctctggctt gctggaccag ctcgaagaga tacccatcga cgcgtaacca aacgttcaat
     5701 cttctgtcaa agcagcccca gcctaagatc gggtataagc cctgctggac aaatccaacg
     5761 ttccatgtat caaaacagga tacttgttga tatattttcg gtttgtggtt ggcacaaatc
     5821 ttgcagctaa atcagaacaa tttagtctcc ctgagtaata cgagacactc gccgcattca
     5881 gggagagatg ccctggaggt tccacagatc tccagggcat tttttatatt attgacgttt
     5941 accaaagttg gttaagtttg ag
//
LOCUS       DBNGSX010000076         5183 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_212828, whole genome shotgun sequence.
ACCESSION   DBNGSX010000076 DBNGSX010000000
VERSION     DBNGSX010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5183)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5183
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_212828"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="AC16CB_02340"
     CDS             <1..754
                     /locus_tag="AC16CB_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341335.1"
                     /translation="EPLHEPLLHDSRVRLRTDYVYRKDLQPATDDWPYLYLQKPTLPS
                     NYWIGLAGVILISLFMVTRAFPNLRQFNHHFFFMGAAFFLLETKSITEMALLFGSTWI
                     VNAVVIAGILVMIVISNLIVLYYKISNPNPWYIALFASLLVNFFIPVSNYLGMQLFWR
                     IGLATIIQVSPLFFAGMIFATTFSKTSSIESALGSNLLGSVLGGVLEYTSLTYGIRSL
                     YLLAFALYVISMFVLNSSRFTQKQVEDVVASH"
     gene            complement(790..1605)
                     /locus_tag="AC16CB_02345"
     CDS             complement(790..1605)
                     /locus_tag="AC16CB_02345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3341336.1"
                     /translation="MSIDKEKIRLAAEQETLLITLYSKVYGCPQTVFKDEKTRQILDR
                     IDYDFSQLKVPTGTRLTVCLRAKKFDEIVREFLASHPQGVVLHLGCGLDTRFVRVGSP
                     RIDWYDLDLPEVIDLRKKFFDETDNYHMIPSSVTDLSWVDRIPTRDQDVLVVAEGLLM
                     YLKDEQVKALVLRLKEAFPGCELAFDAYSTLTVRRVKDHPSIKKTGALIQWGIDDARQ
                     IEHWASGIRLKEEWFYTQSKDIKKLAFSHRLIFAFVGLFPVALKAHRILYYAL"
     gene            complement(1646..2554)
                     /locus_tag="AC16CB_02350"
     CDS             complement(1646..2554)
                     /locus_tag="AC16CB_02350"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR3341337.1"
                     /translation="METIITSPQKVVKIGPEHPFVVIGERINPTGRKLLAAEMAAWNF
                     DRVISDAIAQVQAGARMLDVNAGIPLADEPAILAEAVRVVQSVVDVPLSIDSSVIKAL
                     EAGLAAYNGKPLVNSVTAEEDRLEVVLPLVKKYGAAVIGIANDETGISEDPDVRFTIA
                     KKIVERAEDYGIPRQDVLIDPLAMPVGAVRMAGVTLFTLVRRLRDELGVNTVCGASNI
                     SFGLPERGTLNATFLAMAISAGMTCAITNPLEESIRKTVLAADVMMGRDENCLAWLSA
                     HREEAPGESGSARQRRRSRREELSRK"
     gene            complement(2558..3577)
                     /locus_tag="AC16CB_02355"
     CDS             complement(2558..3577)
                     /locus_tag="AC16CB_02355"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008205181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - L-methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HIR3341338.1"
                     /translation="MDQRLPSTTEDGTSWVGRVRSGSRLERVLRSGRFAVTAELNPPD
                     SADPQSVYDRALVLAEVCDAINATDASGANVHMSSVGVCALLTRAGYEPVMQVSCRDR
                     NRIAIQGDLLGAAAMGVRNVLCLTGDGVQVGDQPEAKPVFDLESITLLRTARMLRDQG
                     HFLSGRQLDVKPKFFLGAAENPFIPPYDFRSLRLAKKIEAGAEFIQTQYCFDFRRLCK
                     FMDSVRDMGLHEQAYILVGVGPLRSAKAAEWMRSRVPGVVIPDEIIRRLNGVPSSRQW
                     EEGKRICIEIIQQVREVEGVSGVHVMAYRQEELVAEIIQEAGLLPRRRISELGQYDPC
                     EEYTI"
     gene            complement(3502..3966)
                     /locus_tag="AC16CB_02360"
     CDS             complement(3502..3966)
                     /locus_tag="AC16CB_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HIR3341339.1"
                     /translation="MKDSPHFLEVVYRSVYRMLLPFRPWLKPDGWVERVFVQLERLSK
                     GALFDCRMCGQCVLHYTGMTCPMTCPKNLRNGPCGGVRLDGNCEIYPEKACVWVQAWQ
                     RSQQMPAYGGEILHIQAPLNYSLKDTSAWINDFHLQQKMAPVGWDESGQVPA"
     gene            complement(3979..4281)
                     /locus_tag="AC16CB_02365"
     CDS             complement(3979..4281)
                     /locus_tag="AC16CB_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011975919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HIR3341340.1"
                     /translation="MTVTYQIVYWRDIPAQIRLRSPQGRISRPLSPRFQEAIDAAAMR
                     AHATSTDDYLEDWRTGDTQSREGDAQQVADSVADEIEAAYPQDRLLGLAANGGLEK"
     gene            complement(4278..4859)
                     /locus_tag="AC16CB_02370"
     CDS             complement(4278..4859)
                     /locus_tag="AC16CB_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HIR3341341.1"
                     /translation="MKTALIACGALAREVLALKEKHGWDVEVLALPSLLHNEPARIPG
                     AVQERIRQARESHDRVLVVYGDCGTGGLLDRLLAKEGVQRVAGPHCYEMYADGTFERL
                     MAEAPGTFFLTDYLVQSFDHLVLEGLGIDKHPELRDEYFRNYTRVVYLAQRDDPALLE
                     RARWAADCLALPLEVHKAGYGKLESRLKELIEA"
     gene            complement(4856..>5183)
                     /locus_tag="AC16CB_02375"
     CDS             complement(4856..>5183)
                     /locus_tag="AC16CB_02375"
                     /inference="COORDINATES: protein motif:HMM:NF014615.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR3341342.1"
                     /translation="PEDAATTLSSYGAVAVGGNCGNGVEEIITVVEKMHAVEPEAILV
                     AKANAGIPKLVGGQAVYAATPQDMADYAVKAFAAGARVIGGCCGSTPAHIQAISTALA
                     SQVVAG"
BASE COUNT         1121 a         1578 c         1284 g         1200 t
ORIGIN      
        1 tgaaccgcta catgagcctt tattgcatga ctcacgtgtc cggctgcgga ccgattatgt
       61 ctatcgaaaa gacctgcaac ccgctacaga tgattggcct tatctgtatc tgcaaaaacc
      121 tactctaccg tcgaattact ggattggttt ggctggtgta atccttatta gcctttttat
      181 ggttacacgt gcttttccca atcttcgaca gtttaaccac cactttttct tcatgggcgc
      241 agcttttttc ctgcttgaga ccaaaagcat taccgagatg gcgctgttat ttggctccac
      301 ttggattgtc aatgctgtgg ttattgctgg aatacttgtg atgattgtca tttctaatct
      361 aattgttttg tattacaaaa tctccaaccc aaatccttgg tacattgcct tgttcgctag
      421 cttattggtt aacttcttta tacctgttag caattacttg ggaatgcaac tattctggcg
      481 aattggttta gctactatta ttcaggtaag cccgttattt ttcgcaggca tgatctttgc
      541 cactaccttt agcaagacct catccataga gagtgcgtta ggctcaaacc ttttaggttc
      601 tgtcctagga ggcgttctag aatatacgtc actgacttac ggcattcgca gcctatatct
      661 gcttgctttt gcactatatg taatttcgat gtttgtgctt aattcgtcta gattcaccca
      721 aaaacaagtt gaggatgtag tagcatccca ttaacaaaat gaagtaccta aatcttctac
      781 attctcttct tacagcgcgt aatacagaat tcgatgggct ttaagcgcca cagggaagag
      841 cccgacgaat gcgaatatca agcgatgact gaaggcgagt ttcttaatat cctttgattg
      901 tgtgtagaac cattcttctt ttagacggat gccagatgcc caatgttcga tctgcctggc
      961 atcatcgatg ccccactgga tcaaagcccc ggttttcttg atagaggggt ggtctttcac
     1021 tcttctaacg gtcagggtgc tataggcatc gaatgccagc tcacatcctg gaaaagcctc
     1081 tttcagcctg agaactaacg cctttacctg ctcatctttt agatacatca gcaaaccctc
     1141 ggcgacaacg aggacatcct gatcccgagt aggaatccgg tcgacccaac tcagatcagt
     1201 gacagaagac gggatcatgt ggtaattgtc tgtctcgtca aaaaacttct ttctaaggtc
     1261 aatcacctcg ggcaaatcca ggtcgtacca gtcgatcctt ggggaaccaa ccctgacaaa
     1321 gcgagtgtcc agcccgcacc ccaaatggag cactacaccc tgcggatgac tggcaaggaa
     1381 ttcccgcaca atctcatcaa acttcttggc gcgcaggcaa accgtcaggc gcgtccccgt
     1441 ggggactttc aattgagaaa aatcataatc aatgcgatcc agaatctgac gcgttttctc
     1501 atctttaaag acagtttgag gacaaccata gaccttggaa tatagcgtga tcagcagggt
     1561 ttcttgttct gctgccaggc ggattttctc tttatcgatc gacatctact taccctcgaa
     1621 aaatcccccc caaattcatc aatgtttact tgcgcgataa ttcctctcta cggctgcgcc
     1681 ggcgctgtct tgcgctgccc gattcccctg gagcttcctc acggtgggcg ctcaaccatg
     1741 ccaggcagtt ctcatcgcgc cccatcatca catcggctgc cagcacagtc ttgcggatgc
     1801 tctcctccag cgggttggtg atggcgcagg tcatcccggc cgagatcgcc attgccagga
     1861 aagttgcgtt caatgtgccc cgctccggca agccaaagga gatattcgac gctccgcaca
     1921 ccgtgttcac gcccaattca tcgcgcagcc tgcgcactag ggtaaagagc gtaacgcccg
     1981 ccatgcgcac cgcccccacc ggcatagcca gcgggtcgat cagcacatcc tggcgcggga
     2041 taccgtagtc ttccgcccgc tccacaatct ttttggcgat ggtaaagcgt acatccgggt
     2101 cctcagagat tcccgtctca tcgttggcga tgccgatcac tgccgccccg tacttcttta
     2161 ccagaggcag caccacttcc agccgatcct cctcggcagt caccgagttc accaatggct
     2221 tgccattgta agcagccagg cccgcctcca gcgctttgat caccgacgaa tcgatactca
     2281 gcggcacgtc cacaacgctc tgcaccacgc gcactgcctc tgccaggata gctggctcat
     2341 ccgccagcgg gatgccggcg ttcacatcca gcatgcgcgc ccccgcttgc acctgtgcga
     2401 tagcgtctga gatcaccctg tcgaaattcc aggcagccat ctctgccgcc agcagcttgc
     2461 gcccggtggg attgatgcgc tccccgataa ccacaaacgg gtgctccgga ccgatcttaa
     2521 ccactttctg aggtgaggtg atgatcgttt ccatagttta aatcgtgtac tcctcgcacg
     2581 ggtcgtactg ccccaactcg ctaatccgac gacgcggcag cagcccggct tcctgaataa
     2641 tctcagccac cagctcttcc tgccggtagg ccatcacgtg cacgccgctc acaccttcga
     2701 cttcgcgcac ctgctggata atctcgatgc agatgcgctt gccctcctcc cactgacgtg
     2761 atgatgggac gccattcaag cggcggatga tctcatcggg tatgaccaca cctggtacgc
     2821 ggctacgcat ccattccgcc gcttttgctg agcgcagcgg gcctaccccc accaagatat
     2881 aggcctgttc gtgcaatccc atatctcgta cagagtccat aaatttgcat aagcggcgaa
     2941 agtcgaaaca atactgcgtc tggatgaact ccgccccagc ctcgattttc ttggccagcc
     3001 gcagggagcg gaaatcgtag ggtggaataa atggattctc ggctgccccc aaaaagaact
     3061 tgggtttcac atccagttgc ctgccgctta aaaagtgccc ctgatcgcgc agcatgcgtg
     3121 ctgtgcgcag cagggtgatc gattcaagat caaagaccgg cttggcctct ggctgatcgc
     3181 ccacttgcac gccatcaccg gtcaggcaga gcacattgcg cacgcccatt gccgctgcgc
     3241 ccagcaagtc tccctggata gcgattcggt tgcggtcgcg gcaggagacc tgcatgaccg
     3301 gctcgtagcc ggcacgcgtc agcaaagcac acaccccaac gctggacatg tgcacgttgg
     3361 cacccgaggc gtctgtggca ttgatggcgt cgcacacctc tgccagcacc agcgctctat
     3421 catacaccga ctggggatcg gcgctgtccg gggggttgag ttcagcggtc actgcaaacc
     3481 gcccagagcg cagcacgcgc tctaagcggg aacctgacct gactcgtccc acccaactgg
     3541 tgccatcttc tgttgtagat ggaagtcgtt gatccatgcc gaggtatctt tcaaactgta
     3601 gttcagcgga gcttgaatgt gcaggatctc cccaccatag gctggcatct gctgggagcg
     3661 ctgccaggct tgcacccaca cgcaagcctt ttctgggtaa atctcgcagt tgccgtccag
     3721 ccgcacccct ccgcaaggtc cattgcgcag gttcttgggg caggtcatgg ggcaggtcat
     3781 gccggtgtaa tgcaacacgc attgcccgca catgcggcag tcaaaaaggg cgcccttgct
     3841 caagcgctcc aactggacga acactcgctc aacccacccg tctggcttca gccaaggtcg
     3901 gaaaggcagc agcatgcggt acaccgaccg gtaaacaacc tcaaggaaat ggggtgaatc
     3961 tttcaaacga cgaaacactt acttttctag gcccccgttc gccgccaacc ccaacaaccg
     4021 atcctgcggg taagcagctt cgatctcgtc tgctacagag tcggccactt gctgcgcatc
     4081 gccctcgcgg ctctgtgtat caccggtacg ccagtcctca aggtagtcgt cggtactggt
     4141 ggcatgggca cgcatagcgg ctgcgtcgat tgcctcttgg aaacgcggcg aaagtgggcg
     4201 agagatgcgc ccctgcggag agcgcaagcg tatttgggcc gggatatccc gccaatagac
     4261 gatctggtag gtgacggtca tgcctcgatt aattccttca acctgctttc cagcttgccg
     4321 tagcctgcct tgtgtacctc caaggggaga gccagacaat cagctgccca tctagccctt
     4381 tccagcagcg caggatcgtc acgctgggca agatacacca cgcgggtgta attacggaaa
     4441 tattcgtcgc gcagctcggg gtgtttatcg atacccagcc cctccagcac caggtggtca
     4501 aaggactgca ccaggtaatc cgtcaagaag aacgtgccgg gtgcctcagc catcaagcgc
     4561 tcaaacgtgc cgtcggcgta catctcataa cagtgcggtc cagccacgcg ctgcacgcct
     4621 tccttcgcca gcagccggtc gagcaagcca cccgtgccgc agtcgccata gactaccagc
     4681 actcgatcat ggctttctct cgcctggcgg atacgctcct ggactgcccc aggaatgcgc
     4741 gcaggctcgt tgtgcagcaa ggagggcaaa gccagcacct ccacatccca gccgtgcttc
     4801 tccttcagcg ccaacacctc acgcgccaac gcgccacagg cgattagggc ggttttcatc
     4861 ctgctaccac ttgagatgcc agggctgtgg agatggcctg gatatgggca ggtgtgctgc
     4921 cacagcagcc tccaatgacg cgggcgcccg ctgcgaaggc tttaacggcg taatcagcca
     4981 tgtcctgcgg ggtggctgca tacaccgctt gcccgcccac cagcttgggg atgcccgcgt
     5041 ttgccttggc aaccagaatc gcttctggct caactgcgtg catcttctcc acaacggtga
     5101 tgatctcctc caccccgttg ccgcagttgc ctcctaccgc tacggcacca taggatgaca
     5161 aagtcgtggc agcatcttcc ggt
//
LOCUS       DBNGSX010000077         5019 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1321048, whole genome shotgun sequence.
ACCESSION   DBNGSX010000077 DBNGSX010000000
VERSION     DBNGSX010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1321048"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1544)
                     /locus_tag="AC16CB_02380"
     CDS             complement(54..1544)
                     /locus_tag="AC16CB_02380"
                     /inference="COORDINATES: protein motif:HMM:NF053209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptA/MptB family glycosyltransferase"
                     /protein_id="HIR3341343.1"
                     /translation="MSEIIWSKLNQWGGITLLAGVGMTSAFVPLACLPTLRGQNVIVF
                     LSHFLLAGAAYCIAVSRLGRDRLPMRVLWAFAILFRLIFLTTSPTLSDDVYRYIWDGH
                     LLGERVNPYALEVNSPALDAYTIPQRAQVNHNWMASPYLPVAQLFFGSVTRIAPQSVL
                     AFQIASVVLDLFTGWLLVRILKQVALPRIHALIYLWNPLVVVEFSHGAHVDALMLFFI
                     TLALYFIVCVPDGPSRQWWLYTSAIALAAATLTKGLPLIVAPFYLRRWGWRRLIVYVT
                     ILVGVLASCAVGAGWGLFGALDGRGLFGALRIYLNGWSFNSGINYWLETGLSSYQQSG
                     RWPAWAVGADVVRLVRWLTSAAQILVVIVAGMVAWRWDGSPRGEPVERDRRLLRLCAI
                     PIGAYLLLTPTLHPWYVTLLLIFLPFLSPAQGEKMSVGRFIWPWVYFSIAVALSYLTY
                     IDPNNLREFAQVRLIEYIPLYLLLIWAAWPLIRHGKMFKRAVKRIS"
     gene            complement(1600..3153)
                     /locus_tag="AC16CB_02385"
     CDS             complement(1600..3153)
                     /locus_tag="AC16CB_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341344.1"
                     /translation="MKRPDKLRINQIPWHPVIIALFPVLTLLAYNVAEIKLSEGTRSL
                     IGAFCLGVTLLVISRLIIKDWYKAAAVASLALILFSSYGHFYNYLEPISLAGLAVGRH
                     RVLVPVYMALFGLGIWWITRSMVDIKVLTRALNVVSITAIIIPVLQISIYSVREASAE
                     IVEPSTGTQQLSYSESKSPPDIYYIILDGYSRDDVLKNFYGYDNSSFLDELTASGFYI
                     ARCSQSNYSQTQLSLSSALNLNYLSELGQGFTEDRTDRVGMPGLIKHSAVRQLLEDLG
                     YTMVAFETGYYWTQVEDAGVYISSKSSVTNLLDITGGLNGFESLLIKTSAGLILADGA
                     TILPRFMRADLDHPQEIHRQRVLFTLDQLGKLANMPGPKFVFAHIVSPHRPFVFGPQG
                     ESVDHEKEPIIGYRDQVAYLNSRMVPILQKIITDSATPPIIIIQGDHGGVDTSGKDRL
                     AILNVYYLPDGGDQQLYENITPVNTFRLIFNYYFGGQYDLLEDASYSSAYKTPFALTP
                     IPNSRPGCP"
     gene            3468..3962
                     /locus_tag="AC16CB_02390"
     CDS             3468..3962
                     /locus_tag="AC16CB_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3341345.1"
                     /translation="MSNLIRRDPFREMMSLRSAMDRLFDSAFFGTQWEWQSLSGEMAL
                     DVAETENEFLVKASLPGINPDDLEITFSGKTLTIKGEYKIEEEKEDVHYHMRERRFGS
                     FSRSVSLPTPIVADDIEARYESGVLTLHLPKTEEVKPKRITVQRVETPRVIEGKATDI
                     ASKN"
     gene            complement(4082..4726)
                     /locus_tag="AC16CB_02395"
     CDS             complement(4082..4726)
                     /locus_tag="AC16CB_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015143807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR3341346.1"
                     /translation="MIHVAVVAQALALRTGLRALLSDGELIEVVAEATSLDELETLLS
                     DIDVLLLAEPRSLEGLSDLELSGTLPSILLLSDEARAAQDLAGISPHAWGVVPMEATA
                     EELQAAVVALHEGLIVLSPLQLRRGAALNRLLGLPEGEVQGLAEHPIEPLTERELQVL
                     QLLAQGLANKQIAAALSISEHTVKFHVSSIYAKLGAANRTEAVRLGVQQGLVIL"
     gene            complement(4723..>5019)
                     /locus_tag="AC16CB_02400"
     CDS             complement(4723..>5019)
                     /locus_tag="AC16CB_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR3341347.1"
                     /translation="QIVLAVGHPWGQRGVVTAGIISGLGTAQTRDQRQSVPIIRSDVV
                     LAPGNSGGPMVNVAGGVVGINTMIVGGDQGVAIPSHVVAAFVRRALKQNEEVTA"
BASE COUNT         1357 a         1346 c         1235 g         1081 t
ORIGIN      
        1 gtctattttc ttaaaaatac tttttagcat ttcttctcct gggaatattc cctctagctg
       61 atccgtttta ctgcgcgctt aaacatctta ccatgccgga tcaaaggcca ggctgcccaa
      121 atcaatagca ggtataaggg gatgtattcg atcaagcgca cctgggcgaa ttcgcgcagg
      181 ttattcggat caatgtacgt caggtaggac aatgcgacag caatcgaaaa ataaacccaa
      241 ggccatataa atcgccccac agacatcttt tctccttggg cgggcgacaa gaaggggagg
      301 aaaatgagga gcagggtcac ataccaggga tgtagggtcg gggttaacaa caggtaggca
      361 ccgattggga tcgcgcacag acggagcagc ctgcgatccc tttcaactgg ctcaccccga
      421 ggcgaaccat cccaccgcca ggcgaccatc ccagccacta tcaccacaag gatctgtgca
      481 gcactcgtca accaccttac caggcggacc acatccgctc ccacagccca ggcaggccac
      541 ctgccgctct gctggtaact gctcaacccg gtttccagcc aataattaat cccgctatta
      601 aagctccacc cgttcaagta gatgcgaaga gcgccaaaca gtccccgacc atccagcgcg
      661 ccaaatagcc cccatcctgc cccaacagca cacgacgcca atactccaac caggattgtg
      721 acatagacga tcagccttcg ccagccccaa cggcgcaaat agaaaggcgc cacaataagg
      781 ggcaagcctt tggttaacgt cgcagccgcc agggcaattg cagatgtgta caaccaccat
      841 tgtctcgacg gaccatcagg cacacagacg atgaaataaa gcgcaagcgt aataaaaaag
      901 agcatcaatg catccacatg cgccccatga gaaaactcca ctacaaccag tggattccac
      961 aaatagatca aggcgtgtat ccttgggagc gcgacctgtt tcagaattcg caccaacaac
     1021 cagcctgtaa ataggtctag cacaaccgaa gcgatctgaa atgccagcac gctctgcggc
     1081 gcaatcctgg taaccgatcc aaaaaataac tgagcaactg gtagataggg ggaggccatc
     1141 caattgtgat tgacctgggc tcgctgtgga atggtatagg cgtccaaggc gggtgaattc
     1201 acctctagtg cataggggtt taccctctct cctaacaagt gaccatccca tatatatcga
     1261 taaacgtcgt cggagagggt gggcgaggtg gtcaagaaaa tcagccggaa caggatggca
     1321 aaagcccata gcaccctcat cgggagcctg tcgcggccca atcgactcac tgcaatacag
     1381 taagctgcgc ccgctagcag gaaatggctc aggaagacaa taacattctg gcccctcagc
     1441 gtggggaggc aggcaagcgg cacaaaagca gatgtcatcc caaccccagc caggagcgta
     1501 attccacccc actggtttaa tttactccaa ataatttcgc tcacagtatc tggtagccaa
     1561 ctatcctgca tattcctggc attttattgt gcgcgagctc tacggacagc caggacgcga
     1621 attcgggata ggagttaatg caaagggagt cttgtaagca gaagagtagc tggcatcttc
     1681 gaggagatca tactggcctc caaaatagta attgaagatc agccggaagg tgttgacagg
     1741 ggtgatgttt tcatacaatt gctggtcgcc tccgtctggc aggtagtaca cattcaagat
     1801 cgccaagcgg tccttgcccg atgtatccac gcctccatgg tcaccttgaa taatgataat
     1861 tggcggtgta gcagaatcag tgataatttt ctgtagtatg gggaccatgc gcgaattcag
     1921 ataggcaacc tgatcgcggt atccaatgat gggttccttc tcgtgatcca cactctcgcc
     1981 ctgtgggcca aatacaaagg gccgatgggg ggatacgata tgggcaaaga cgaatttcgg
     2041 ccctggcatg tttgccagct tgccaagttg gtcgagggtg aacagcactc tctggcgatg
     2101 gatctcctgg ggatgatcta ggtcggcgcg cataaatctg ggcaagatcg ttgctccatc
     2161 tgccagaata agcccggcgg aagtcttgat gagcaaggat tcaaaaccat tcaaaccccc
     2221 ggtaatatcc aacaggttcg tcacgctgga ttttgatgaa atgtagacgc ccgcgtcctc
     2281 cacctgggtc caatagtaac cggtttcaaa ggccaccatg gtgtacccaa gatcttcgag
     2341 caattgtcga accgcgctgt gcttgatcaa accgggcatc cccacccgat cggtccggtc
     2401 ttccgtaaag ccctggccca attcagatag ataattcagg ttcagcgcag aagagagaga
     2461 aagctgggtc tgggaatagt tgctctggct gcagcgagca atgtaaaaac cagatgcagt
     2521 caattcatcc aggaaggagc tgttatcgta accatagaag tttttcagga cgtcgtcacg
     2581 cgaatagcca tccaggatga tatagtaaat atctggggga gatttacttt ctgaatagga
     2641 taactgctgc gtgccagtag atggttcgac aatctcagcg ctcgcctctc gaacagaata
     2701 aatagagatt tgcaatacag ggatgattat cgcagtgatc gagacgacat tcagagcgcg
     2761 tgtcagaacc ttgatgtcaa ccattgatcg agttatccac catattccca acccgaataa
     2821 agccatatag acagggacca gaacgcggtg ccggccaact gcaagcccag ccaggctgat
     2881 tggctccagg taattataga agtgaccata ggaggagaac agtatcagcg ccagcgaagc
     2941 aactgctgct gctttatacc aatccttgat aatcaatctg gagataacga gaagtgtgac
     3001 gcccaggcaa aatgccccga tcaaggagcg tgtcccttca gatagcttaa tttctgcaac
     3061 attataagcc aatagagtca acaccggaaa aagcgcaatg ataacagggt gccatggaat
     3121 ttgattgatt cgtaatttat ctggtcgctt cataaacaat tgcccaatac agtaagaggc
     3181 gggcacctta ttgagaataa atacccaagg tcagctgctc taattggtat cggagggtga
     3241 ggccgctcag caggccgaag gttgggtagg tgaaatatta tagtccaaac taagggcaac
     3301 ggattgacga gcctcaatcc cctctatttc ccaccagcaa gtgcatagat ttacaaacct
     3361 catatataaa tcctatcttt ttctaatacc cattgggtaa gatgatattg caaggaaacc
     3421 gaacaatagt aaaccataaa taatttacca gtaaaggaga ttaaaccatg agcaacttaa
     3481 ttcgtagaga tcccttccgg gagatgatgt ctttgcgcag tgcgatggac cggctttttg
     3541 acagtgcttt cttcggcacg caatgggagt ggcaatcact aagtggtgaa atggccctgg
     3601 atgtagccga gactgagaat gagttcctgg tcaaagcctc cttgcctggc atcaaccccg
     3661 acgacctgga gatcaccttt agcggtaaga cgctgactat caaaggcgaa tacaaaatcg
     3721 aagaggaaaa ggaagacgtc cattatcaca tgcgcgagcg ccgttttggc tccttctctc
     3781 gcagcgtatc cttacccacg cccatcgtgg cagatgatat tgaggccagg tacgagagtg
     3841 gtgtacttac cctgcacctg cccaagaccg aagaggtcaa gcctaagcgc atcacagttc
     3901 agcgggtaga aacgccgcgg gtgatcgaag gtaaagctac agacatcgca agcaagaatt
     3961 aattacccgt aacgggaaaa acggtctgtc tcgctgcatt actgcgacga gacaggcctt
     4021 tttttgtcca ttatttctct ctttctgccc taaacagtga aagatagaaa ttgaggtatt
     4081 tctacaagat caccagccct tgttgaaccc ccagtctgac cgcctccgtc cgatttgcag
     4141 ctcccagctt tgcataaatc gaggagacgt gaaattttac cgtgtgctcg ctgatgctta
     4201 gcgcggcggc gatttgtttg ttagccaagc cttgagccag taattgcagc acctgcagct
     4261 ctcgctctgt gagaggctca atcgggtgtt cggctagccc ctgtacttcg ccttcaggca
     4321 atcctaacag gcgattcagc gctgcccccc gtcgcagttg cagcggggaa agcacgatca
     4381 agccttcatg caacgcaact actgctgcct gaagttcttc tgcagtggcc tccattggaa
     4441 caacccccca ggcatggggt gaaatcccag cgagatcctg tgcagcacgg gcttcgtctg
     4501 atagcaacag tattgatggg agcgtcccgc tcaattctag atcgctcaac ccctccaggg
     4561 atcgaggttc tgccaacagc agaacatcaa tgtcggacaa caacgtctct aactcgtcca
     4621 gggaggtagc ctcggcgacc acctcgatca actcgccatc actcaggagg gctcgtagcc
     4681 cagtacgcag tgccagcgcc tgggcaacta ccgcgacatg gatcacgcgg tgacctcctc
     4741 attttgtttc agagcccgtc tcacaaaagc cgccaccaca tggctaggga tcgccactcc
     4801 ttgatctcca cctacaatca tggtattgat acccaccacg ccgcctgcta cgttcaccat
     4861 aggcccgccg gaattgcccg gtgcaaggac cacatcggag cggataatag gcactgattg
     4921 gcgctgatcg cgcgtttgag cagttcctaa accgctgatg atcccagcgg ttaccacacc
     4981 ccgctgaccc cagggatggc ccaccgccag cacaatctg
//
LOCUS       DBNGSX010000078         5996 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_213077, whole genome shotgun sequence.
ACCESSION   DBNGSX010000078 DBNGSX010000000
VERSION     DBNGSX010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5996)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5996
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_213077"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="AC16CB_02405"
     CDS             complement(<1..223)
                     /locus_tag="AC16CB_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341348.1"
                     /translation="MMKNWHFRSNTVPIALFAYCLISFGLLIPWLGFYWDDWPSIYYL
                     HVLGPRGFIDAFAVDRPTLGWFFMLTSSIV"
     gene            complement(412..3180)
                     /gene="alaS"
                     /locus_tag="AC16CB_02410"
     CDS             complement(412..3180)
                     /gene="alaS"
                     /locus_tag="AC16CB_02410"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIR3341349.1"
                     /translation="MTNSLSGSKIRQEFIDYFVNKAGHTFVPSSSLVPGGDQTLLFTN
                     AGMVQFKDVFLGTDKRPYTRAANSQKCMRVAGKHNDLDDVGRDDTHHTFFEMLGNWSF
                     GDYYKKEAIAWAWELLTDIWGLPRQQLWVTCFRDDIGDIPQDDEAASEWTKQPGMDPS
                     HVLFFGRKENFWEMAEVGPCGPCSEIHIDRGPQYCNKQAVPGHVCRVNGDCTRFLELW
                     NLVFIQYNRHSPTMLEPLPAMHVDTGMGLERIVSVLQDVDSNYKTDLLNPMMDAVQRL
                     TGHNQAERRANVTPYRVIADHSRAAAFLIADGVVPGNVGRNYVCRMIVRRAARFGSKI
                     GLDEPFLAKVAEVVIDTYGDFFPELNRNRTSILDNLTREEIRFQRTVEGGVTKLENLL
                     TRLKIQGGIVLSGDQAFDLYATYGLPLEITRDIAREQNLEVDEPAFRRAMEEHRLASG
                     AGETFGPLGGEDVDIYRKLLEELQGQGKLGDSGVAYDPYSTVEVEGPVLALVKEGQII
                     SSASQGEKVEIVLPETCFYVEAGGQVSDTGSIVAAGKPDSSETSRSMWIIHISEMYKP
                     AAGMIVHAGEVLQGEVKTGDQAIARVDVQRRHDIMRNHTATHLLHHELRAVLGEHARQ
                     AGSLVAPDRLRFDFTHPEALTTQQLEQIEAGVNRNILSNYRLSIAFKPLQQAIAEGAI
                     ALFGEKYAETVRNITIGDAEPFSNELCGGTHVDETGDIGTFLITNEGSAAAGIRRIEA
                     VTGRGAYNLIQRRFRALKQSAALLAAPPEETPTKTQALLDELENARKQVAALRSELAA
                     SDFAQHLEDVPLIVDVPVLVAKLPGADVDTLRLMTDRFRERYPSGVAVLASIIEGRPI
                     LVAAVTQDLIERGLHAGELIKFVAQPLGGGGGGRPNLAQAGGKDASQLDKALGSVAGW
                     VAEKLGAD"
     gene            complement(3239..3679)
                     /gene="mscL"
                     /locus_tag="AC16CB_02415"
     CDS             complement(3239..3679)
                     /gene="mscL"
                     /locus_tag="AC16CB_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HIR3341350.1"
                     /translation="MLKEFKEFAMRGNVLDLAIGIIIGAAFGKIITSFVNDVLMPPIG
                     LLLGGVNFANFFLTLSGPTVKTLEEAQAAGAVTLNYGVFINTIIDFLIVAFAIFLLIR
                     QVNRLQRQPAPEPAAPTTKECPYCFTSISIKAVRCPNCTSQLEE"
     gene            3831..5384
                     /locus_tag="AC16CB_02420"
     CDS             3831..5384
                     /locus_tag="AC16CB_02420"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HIR3341351.1"
                     /translation="MTGYLYFAVFASGMTTLAIELSASHLLGSVFGTSNLVWASIIGL
                     ILIYLTAGYFIGGRWADRSPHERTMYTVMAWGAFTSGLVPVAAYPVLRLAADSFDQLQ
                     MGVLFGSFAAVLVLFILPVTLLGTISPFAIRLAIRDTQSAGVTSGRIYAISTLGSFVG
                     TFLPSLILIPLVGTRRTFLIFSAFLVLTAFGGLWRSSGWRGVLRLLWMPVLLILLDIL
                     GAGRPIKSTSGQIFETESAYNYIQVLERDGYRLLRLNEGQGIHSMWHPTQIAYEGPWE
                     QFLVAPFFNPPRYEPERVASMAILGLAAGTTARQATAVFGAIPIDGYEIDPKIIEVGR
                     EYFGMTMPNLNPIAQDGRVGLARSPKRYTVIGIDAYRPPYIPWHLTTREFFQQVQEHL
                     TDDGTLVINVGRSPTDRRLIDGLVGTISSIFPSIYVIDVPNTFNSIVYATNQPTNIEN
                     LYENWLYLKTRQDVHPLLLHAIELAIIHQQPTPESDIVYTDDLAPVEWVTNSMILNFV
                     LKGDMNQLR"
     gene            5381..>5996
                     /locus_tag="AC16CB_02425"
     CDS             5381..>5996
                     /locus_tag="AC16CB_02425"
                     /inference="COORDINATES: protein motif:HMM:NF018964.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein intramolecular transacylase Lit"
                     /protein_id="HIR3341352.1"
                     /translation="MNAQSQSSKLNTQIIKILSWLVTLLMPVVLVMAAVRLLLTPAFL
                     IIEYNTPGFPPDPYGFTKSERLTWSKIAVEYLLNDADVNFLGDLRFPDGKQAPAESCR
                     SMDDCTRLYNDRELKHMLDVKIVTQAAVRVWYISLAVLLLLYIWAWRGGWWGAFRQGM
                     GRGGWLTVFLLAGIILFVMLAFGVIFVAFHNVFFEAGTWQFLFSD"
BASE COUNT         1388 a         1520 c         1533 g         1555 t
ORIGIN      
        1 ccacaattga cgaagtgagc atgaaaaacc agcccagagt tgggcgatct actgcgaacg
       61 catcgataaa cccccttggg ccgagcacat gaagataata aatcgaaggc caatcatccc
      121 aataaaatcc caaccaaggt atgagtaacc caaagctaat caggcaatag gcgaaaagtg
      181 cgatagggac tgtgttcgaa cggaaatgcc agtttttcat catcgctggg acttatttga
      241 tggaattata acgtaaaagg gtaatgccct cccccgaggg cattgaattg tctcaccccc
      301 ctctttacat tcagagagac ggttcaagag actggggttg ggatccgatt tctacccgag
      361 tacccattat ttgggaataa attgcttttt tgatggagag ttgtctccag gctaatcagc
      421 gcctagcttc tcagcaaccc agccagctac gctacccagg gctttatcca gctgagatgc
      481 gtcttttccg cctgcctgtg ctagatttgg acggcctccg cctccgccac ccaatggctg
      541 ggcaacaaac ttgatcagct caccagcgtg gaggcctctt tcaatgagat cttgtgtaac
      601 tgccgccacc aaaattggac gtccttcaat aatcgatgcc agcacagcca cgccgctagg
      661 atatcgctcc ctgaagcgat cggtcatcaa tcgcagcgta tcgacgtctg cccccggtag
      721 tttggcaacc agcactggaa cgtcgacgat caaagggaca tcctctaagt gttgggcgaa
      781 atcgcttgcg gctagctcgc ttcgcaaagc agcgacttgc ttccgagcgt tttccagttc
      841 gtccagcaag gcttgggtct tagttggggt ctcttcaggg ggcgcagcca gcaaagctgc
      901 agattgtttt aacgcgcgaa agcgacgctg aatgaggttg tatgcgccgc gcccagtcac
      961 agcctctatg cggcggatgc ccgcagctgc gctgccttcg ttggtgatca aaaaagtgcc
     1021 aatgtcgcct gtctcgtcga cgtgcgtacc tccacatagc tcgttggaaa atggttctgc
     1081 atcgccaatg gtgatgttgc gaaccgtctc agcgtatttt tcaccaaaaa gtgcaatggc
     1141 gccttcagcg atagcctgct gcaatggctt gaatgcaata cttaacctat agttgctcaa
     1201 tatattgcgg tttaccccag cctcgatttg ctcaagttgt tgagtggtaa gcgcttcagg
     1261 atgggtgaag tcaaagcgca gcctatccgg agctaccagg gaaccagctt ggcgagcgtg
     1321 ctcaccaagt acagcgcgca attcgtgatg gagcaagtgg gtggcagtgt ggttacgcat
     1381 aatatcgtgg cgtctttgga catccacgcg ggcgatagct tgatcacccg tcttcacctc
     1441 gccttgtagc acctcgcccg catgcacgat cattcctgcg gctggtttat acatttcgga
     1501 aatatgtatt atccacatcg atctactggt ctcggaggag tcgggtttgc ccgccgcgac
     1561 gattgaacca gtatcggaga cttgaccacc agcctcgaca tagaaacagg tttctggcag
     1621 tacaatctct accttctctc cctgcgaagc gctcgaaata atttgccctt ctttgaccag
     1681 agcaagaact ggaccttcca cctcaacggt actataagga tcatatgcca ccccgctatc
     1741 cccaagcttg ccttgtcctt gtagctcttc caggagcttg cggtaaatat ccacatcctc
     1801 cccacccagg ggaccgaagg tttcacctgc gccagaagcg aggcggtgct cctccattgc
     1861 cctgcgaaaa gcgggttcat ctacctcgag attttgttcc cgggcgatat cgcgggtgat
     1921 ctcaagcggt aggccatagg tagcgtacaa gtcaaatgcc tggtcacccg ataagacaat
     1981 tcccccctgt atcttcaggc gagtgagaag gttttccagt ttggtcacac caccttctac
     2041 agtcctttgg aagcgtatct cctcgcgggt taagttgtcc aggatacttg tgcggtttcg
     2101 attcaactcg ggaaaaaagt cgccgtaggt gtcgatgacc acttcagcca ccttggcgag
     2161 aaacggttcg tccaacccaa ttttactgcc gaagcgggca gcacggcgca cgatcatacg
     2221 acatacgtag ttgcgtccta catttcctgg tacgacgcca tcagcgataa ggaatgcagc
     2281 tgcacgggaa tggtcagcga taacacggta aggagtgaca ttagctcgtc tctcagcctg
     2341 gttatgccct gttaagcgct gtaccgcatc catcattgga ttgagtagat cggttttata
     2401 gttggagtcg acatcctgga gcaccgaaac gatacgctcc agccccatgc cggtatcgac
     2461 gtgcatagcg ggcagtggtt ccagcatcgt ggggctatgg cggttatact ggatgaacac
     2521 caggttccac agctccaaga aacgggtgca gtcaccgttg actcgacaga cgtggccggg
     2581 gacagcttgt ttattgcaat attgtggacc gcggtctatg tggatctcgc tgcatggacc
     2641 gcaggggccc acttcggcca tctcccagaa attttccttg cgcccgaaaa agagcacatg
     2701 agacgggtcc atgcccggtt gcttggtcca ctcactggca gcctcatcgt cttgtgggat
     2761 atctcctata tcgtcacgaa aacaggttac ccaaagctgc tggcgcggca gcccccaaat
     2821 atcggtgagc aattcccaag cccaagcgat agcctctttc ttgtaatagt ctccaaagga
     2881 ccaattcccc agcatttcga agaacgtgtg atgcgtgtcg tctcgaccga catcgtcgag
     2941 atcattatgc ttgccagcta cgcgcatgca cttttgtgaa ttggcagctc gtgtgtatgg
     3001 tcgcttgtcg gtgccgagga agacatcctt aaattgcacc atacctgcgt tggtgaaaag
     3061 gagcgtctga tccccacccg ggacaagtga ggaggacggc acaaatgtgt gcccggcttt
     3121 attgacgaag taatcgataa attcttgtct aatcttggat ccagataatg agttggtcat
     3181 accatcctaa atgttctttt tatagatatt ctaatcttaa ttcttgatag ccaattcgtt
     3241 attcttctaa ttgcgaagtg caattcgggc agcgtaccgc cttgatcgaa atagacgtga
     3301 aacaatatgg acactctttt gtggtgggag cggcaggttc tggggctggt tggcgttgca
     3361 agcgattgac ctggcgtatc agcaaaaaga tggcaaaagc gacgatgaga aaatcgatga
     3421 tcgtattgat aaatacacca taattgagcg tcactgctcc tgccgcctgg gcctcctcca
     3481 gtgttttaac tgtaggaccg gatagggtta gaaaaaagtt agcaaaattt accccgccca
     3541 gcaataaccc aatggggggc ataaggacat cgttcacaaa cgaagtaatg attttaccga
     3601 aagccgcgcc gataatgata ccaattgcca ggtcgagtac attgccgcgc atagcaaatt
     3661 ctttaaattc ctttagcatt ttggcctcct tcgaaatatg ggctcggttt gagctattat
     3721 aagtgatgaa aataaattgg gctagtcgga aatcacgtta aaaaaaacac agtgagagat
     3781 gaccttacct tgcgtgcagg gtgaagatag gggataatca ggtactaatt atgacaggat
     3841 atttgtattt cgccgtcttc gcttcgggta tgactaccct ggcgattgaa cttagcgctt
     3901 cacacctgct gggcagtgtt ttcggcacga gcaacctggt ctgggcgagc attattggcc
     3961 tgatcttaat ctacttgact gctggctatt ttattggggg gcgctgggca gaccgctcac
     4021 cgcatgagag gacgatgtat actgttatgg cctggggggc attcacctct ggtttagttc
     4081 cggtagccgc ttacccggtt ttgcgcctgg cggcagactc cttcgatcag ttgcagatgg
     4141 gcgtgctatt tggttctttt gctgctgttt tagtgctttt tatcttaccg gttacgctgc
     4201 tgggaacgat ttctccattt gccattcgtc tggcgatccg tgatacacag agcgcagggg
     4261 tgacctctgg gcgcatctat gccatctcca ccctgggctc atttgtgggc acatttttac
     4321 cgtccttgat attaattcct ctggttggca cgcgccgtac ttttcttatc ttcagcgcgt
     4381 tcttggttct cactgccttt ggtggactct ggcgttcatc tggctggcgg ggtgttctgc
     4441 gcctcctgtg gatgccggtc ttattgatat tgctggatat cctaggtgca gggcgaccca
     4501 ttaaatccac ctcaggccag atcttcgaga ccgaatcggc ttataactat attcaggtac
     4561 tggagaggga cggttaccgt ttgttgcgtt tgaatgaagg acaaggtatc cactccatgt
     4621 ggcatccaac ccaaatcgcc tatgaaggcc cttgggagca gttcctggta gcgccatttt
     4681 tcaaccctcc caggtatgag ccggagcgag tagcgagtat ggcaatcttg ggattggcgg
     4741 caggcacgac tgccaggcag gcgacagcgg tttttggagc catccctatt gatggttacg
     4801 aaatcgatcc gaagataatt gaggtaggcc gagaatattt cggcatgacg atgcccaacc
     4861 tgaatcctat tgcccaggat gggcgcgtgg gactggcgcg cagtccaaag cggtacacag
     4921 taattggtat tgacgcctat cgaccaccat atatcccctg gcacttgaca acgcgagaat
     4981 tttttcagca agtgcaggag cacttaaccg atgatggaac cctggtgatc aatgttggtc
     5041 gctcgcccac ggatcgccgt ctgatcgacg ggctagtggg cacaatttcc tcaattttcc
     5101 cttcaatcta cgtgatagat gtcccgaata cattcaattc gattgtatat gctaccaacc
     5161 agccgaccaa tattgaaaat ctatatgaaa actggttgta tttaaaaact cgccaggatg
     5221 tacaccccct cttgttgcat gccattgagc tagctattat ccatcagcag cctacacccg
     5281 aaagtgatat cgtatataca gatgatctcg ccccggttga gtgggtaacc aactccatga
     5341 tcctcaattt tgtactgaag ggagatatga accaactgcg atgaatgccc aatctcaatc
     5401 ttcgaaatta aacacccaaa taatcaagat cctctcctgg ctggttaccc tgcttatgcc
     5461 ggttgtttta gtgatggcag cagttcgcct tttgcttacc cccgcttttt tgatcattga
     5521 atacaacacc cctggatttc cgcctgaccc gtatggcttt acgaaatctg agcggttaac
     5581 gtggtcgaag attgcagtcg aatacctgct caatgatgca gacgtcaatt ttttaggtga
     5641 cttacggttt cccgatggca agcaggcgcc cgcggagtct tgcagatcga tggacgattg
     5701 cactcgtctg tataatgatc gcgagctgaa gcacatgctg gatgtgaaga tcgtgaccca
     5761 agccgcggtg cgcgtgtggt atatcagcct ggcggtgttg ctactcttat atatttgggc
     5821 ctggcgtggt ggctggtggg gggcattccg gcaaggaatg gggcgaggtg gttggttgac
     5881 tgtgtttctg cttgcaggaa ttatcctttt tgtgatgttg gcgttcgggg tgatcttcgt
     5941 cgcatttcac aatgtcttct ttgaggcggg cacatggcaa ttcctgttta gcgata
//
LOCUS       DBNGSX010000079         9691 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1183201, whole genome shotgun sequence.
ACCESSION   DBNGSX010000079 DBNGSX010000000
VERSION     DBNGSX010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9691)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9691)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9691
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1183201"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(379..1308)
                     /locus_tag="AC16CB_02430"
     CDS             complement(379..1308)
                     /locus_tag="AC16CB_02430"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR3341353.1"
                     /translation="MRKTIVRLLYGFIGLLILAVLAITALIGYDFLFPSQRITDFANV
                     TYPGPQGTTLNGYLARPGDGEPGPALLLIHAFYGLDQDIIQKADLLAQQGYTVLAVDA
                     FRGQTTRRVPRGIWLVVTTPQDEIRADIDAAYTYLAGLESVDPQRIGVVGFCFGGSQA
                     MFLGTRNPDLRATVIFYGGGPITDPAELDFLGESGPVLGIYGAEDQSIPLEQVSAFEA
                     AMNTRGISNQISVYPGVGHAFVHADNIAQPGAAQDAWNEMLTFLSENLGSGVTWDGSG
                     RAQSPDVPGADKRLATSGFHLSHALYHVWSGAR"
     gene            complement(1449..1763)
                     /locus_tag="AC16CB_02435"
     CDS             complement(1449..1763)
                     /locus_tag="AC16CB_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HIR3341354.1"
                     /translation="MTMWLVMLAAGLLTYLTRLSFIMLWGKINIPIWLLRSLRFVPPA
                     VFTAIIFPELFLPQATLDISLGNARLIAGILAALVAWKTRNIVLTILVGMSVLLLIQY
                     FV"
     gene            complement(1760..2461)
                     /locus_tag="AC16CB_02440"
     CDS             complement(1760..2461)
                     /locus_tag="AC16CB_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIR3341355.1"
                     /translation="MTSPRTEFLSGVRDELPLLVGVIPFGMIYGILAVSAGIPAVQAQ
                     AMSLILFAGSSQFITAQLFGHGTPAMVMVLTAAIVNLRHALYSASIAPYTRKLRPAWK
                     WTLAYLLTDEAYAVSIHHYQSHDSHSNPESTGNKHWYLLGAGLGLWSTWQVSTAMGVF
                     IGAQVPASWSLDYSLVLTFIALLVPNLRDRPSLAAALAAGVTAVLAHPLPFKLGLVCA
                     ALVGIITGMWLESRK"
     gene            complement(2576..4525)
                     /locus_tag="AC16CB_02445"
     CDS             complement(2576..4525)
                     /locus_tag="AC16CB_02445"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015408016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3341356.1"
                     /translation="MFHGSGWWSYISSTGEKPRVTWDLLRRVLRYARPYRGQIAGMLS
                     LILVNTSLTLLSPLILRALIDKALPARDINQLMLLASGLLFIPALGGGLNVIQRRLNA
                     MIGEGVIYDLRVALYAHLQRMSLRFFTHTKVGELMSRLNNDVVGAQNAISNTIVAAIT
                     NLVQTSAVLAVMLALEWRLTLVSIAILPLFILAARRLGTRLRDIARQAMDFNAQMNAM
                     MNETLNIGGALLVKLFGRAPVEVSRFELRASKVRDTGVQRASLGASFFVIVGLISAMG
                     TALVYGIGGYLVIQGDFTIGTIVAFGAYLTSLYSALQGLANAPVEFATSIVSFERVFE
                     VIDLPMDIVEKSEARVLSNISGELIFEEVSFRYEVDEQVELSAVRRFGQMDSVTTVLS
                     GERPEHHPRDDEGNEDVPRSQARVIALDKISFRVEPGQLVALVGPSGAGKTTLTYLIP
                     RLYDPTEGRILIDGHDLRQVSLDSLSAQIGMVTQETHLFHDTIRTNLLYARLEATQDE
                     LEAAARAANIHDFIAGLPDGYDTIAGERGYRLSGGEKQRIALARVILKDPRILVLDEA
                     TSHLDSESELLIQEALKRVMSGRTSIVIAHRLSTILAADLILVMDRGRFVERGTHTEL
                     LALNGLYASLYETQFSREKDQIQKA"
     gene            complement(4708..4878)
                     /locus_tag="AC16CB_02450"
     CDS             complement(4708..4878)
                     /locus_tag="AC16CB_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341357.1"
                     /translation="MTTQFTWYGHATMGLEMLLGIGHLIIHAAKQVNDKIFGGHEGTL
                     LESDGLLEQCTS"
     gene            5149..5814
                     /locus_tag="AC16CB_02455"
     CDS             5149..5814
                     /locus_tag="AC16CB_02455"
                     /inference="COORDINATES: protein motif:HMM:NF025559.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HIR3341358.1"
                     /translation="MNPILVKMVYRPAIKRAAYAGLVGRNRDCQRLDKGRFTRAEVDY
                     IMEQVWQGYDQLAPAIPREPKLGNRMNMLLACVTLSCFQALTSAGIERDYAIELIGDV
                     AWKVYEKWGRLTIFVARLLSRHPRERMRMSVNMFLRFPFTPPGYVFERLDSSDGVAFN
                     MLRCPISEYFRAQGASDLCVGSWCNLDFSLAEMWGGWLERKETLAAGCTRCDFRFKTV
                     AQV"
     gene            5897..6958
                     /locus_tag="AC16CB_02460"
     CDS             5897..6958
                     /locus_tag="AC16CB_02460"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR3341359.1"
                     /translation="MTAQKVRVAVNGYGVIGKRIADAVVAQDDMELVGVADVVSDWRI
                     RVAVEKGYPVFASTPQAAEGMRTAGILVAGVLDDLVSQIDIVADATPKKVASANLEKY
                     RAIGVKSVFQGGEKHSLTGHSFVAQANYETAIGRDTTRVVSCNTTSIVRTLGALKTAG
                     LLKKARGVLVRRATDPWESDHGGVMNTVVPETIIPSHQGPDAQTVDPDLDVVTIALVA
                     AHTTSHLHSWSVELTRPVTKEEVLDAFRAAPRIAFLRAGEGIVALNSTLEMMADLGRP
                     RGDMWEVGLWEDSLTVKGDEVFYNYQVYNQAIVVPETIDAIRAITGIEKDGAASIAKT
                     DRAMGLVREFVRRTDRLAV"
     gene            6960..7958
                     /locus_tag="AC16CB_02465"
     CDS             6960..7958
                     /locus_tag="AC16CB_02465"
                     /inference="COORDINATES: protein motif:HMM:NF009498.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose 1,6-diphosphate aldolase"
                     /protein_id="HIR3341360.1"
                     /translation="MKNQLTIGKLRGLQQISDARGIFAMCAMDHRGSMQRMISKAESK
                     KVAYETLAEYKRDLAEYLAPESTGILLDPIYGAAQSIAFGALPGGVGLLVSLEETGYE
                     SDAQGRVTTLLPDWSVEKIKRMGASAVKMLLYYRPDLTENAARQREVTRQVSAECLSW
                     DIPFLIEPIAYAVSEAELDPSTFAARKTSLVVDSARDLTQLGVDVLKAEFPADMRFER
                     DEDKLWAACELLDASSVSPWILLSAGVSFDEFARQVKIACQAGASGFLGGRAIWEEAM
                     HITDRGERRQWLVTVGRDRMRRLGDIAGEYGRPWWKKWAGSMSELMDVSPEWYRRY"
     gene            7983..8444
                     /locus_tag="AC16CB_02470"
     CDS             7983..8444
                     /locus_tag="AC16CB_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341361.1"
                     /translation="MKVRSWYGLTGEIEVENELWHLVRVGGVALKHPPIINLLLRRGL
                     PREDRLYLSFLHEFGHLQTLLVALIHGTLMLFARRRHGRGSLRSLTWKWQALIAHQAV
                     WELAAESYTAYYARPRYRQIYRRYPNPLGRSIFWIGMLGLAVYFTQRAVNS"
     gene            8592..9014
                     /locus_tag="AC16CB_02475"
     CDS             8592..9014
                     /locus_tag="AC16CB_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341362.1"
                     /translation="MDTQHPSKLSDPFFSLIVFSIYSGFMGAVLLLAPRIILPAFQVH
                     EEVNSFTYMLGFVLIASSFYYFASGVGRDRFFAKLTIYTRIASPIVTIILYITGNVPV
                     NYVLLSILDASGGVWTFYALRKTRSGDSLKPLGYAQRV"
     gene            9256..>9691
                     /locus_tag="AC16CB_02480"
     CDS             9256..>9691
                     /locus_tag="AC16CB_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR3341363.1"
                     /translation="MTTEDFIIDLFCPVGDQRPDTKKHSQANHYPSEVVTLAILFALK
                     GMGNRAFYRWLERDYGHLFPDLPGRIRLFRLFNTRRHWTQRFMAEPSLNGLIDTYGIE
                     LLHPRREGHSRRQIGKKGLSNRRWIVGGKLCFVLNHLGLIVDW"
BASE COUNT         2233 a         2515 c         2765 g         2178 t
ORIGIN      
        1 aagatacccg ctctgttgcg ccgtttgaaa ggagaaatcg ttcgcggggg acagctctcc
       61 ctgtttgtta gtgatgagga tgccgacgat tggtcattgg aagagaaagt cgctgcccag
      121 gaagccattc ttggggtgag tgtcatcgcc cacccgctgg agatggtaac tgaccagatt
      181 gctgaagctg gggcactcag tacgctggag gctgcatcga gaattgggca gcctttgcgc
      241 gtggctggaa tgcgccagac ctggcgtcgc agcccaacgg tgcgcgggga ttatatctac
      301 tttatgtccc tggaagacct ggaagggatg ctggatgtgg tcatttcttc cgatgtctac
      361 cggcgctgtc gcaaggagct atctggcccc ggaccatacg tggtagaggg cgtggctcag
      421 atggaagccg ctggtggcga gccttttatc cgcgccggga acatctggac tttgcgccct
      481 tccagaccca tcccaggtaa cccctgatcc caggttctcc gacaaaaagg tcagcatctc
      541 gttccaggcg tcttgtgctg cgccgggttg agcgatgttg tccgcatgaa cgaaggcatg
      601 tccaacaccc ggataaacgc ttatttggtt agagatgcct ctagtattca tggcggcctc
      661 aaaagcactt acctgctcca aggggatgct ttggtcttcc gcaccgtaga tgcccagcac
      721 cgggcctgat tcccccaaga aatccagttc cgcagggtcg gtgatcggtc caccaccgta
      781 aaaaatgact gtggcgcgca ggtccgggtt gcgtgtgccc aggaacatcg cctgcgagcc
      841 gccgaagcaa aagcctacca cacctatccg ttgggggtct acgctctcca atcctgccag
      901 gtacgtgtag gctgcatcaa tgtcggcgcg gatttcgtct tgcggagtcg tcactaccaa
      961 ccagatgccg cgcgggacac ggcgggtggt ctggccgcga aaagcgtcga ctgccaggac
     1021 agtgtatcct tgctgagcaa gcaagtcggc tttttggatg atgtcttggt caagcccata
     1081 aaaagcgtgg atgagcagca gcgctggacc tggttcgcca tcaccaggac gggctagata
     1141 gccgttcagg gtggttcctt gcgggccggg ataggtcaca ttggcgaagt cggtgatgcg
     1201 ctgagatgga aacaaaaagt catagccgat caaggcggta atcgcaagga cagccaggat
     1261 cagcaaaccg ataaacccat aaagcaggcg gacgatcgtt ttacgcataa aatctctccc
     1321 atgccaatcg ctggcttgcc agcgtgttgg ataatggctg ttattgtact ctatcgtaaa
     1381 gttttccctg accttacccc ctttttcatg tgtttacctg agatagtcaa tgccgagcta
     1441 tttgtgtcct aaacaaaata ctggatgagc agcagcacgc tcatgcccac taatatggtg
     1501 agcacgatat tgcgtgtctt ccaggcgacc agcgcagcca ggatgcctgc aatcaggcgg
     1561 gcattgccca gggaaatatc cagggttgcc tgcgggagga acaactcggg gaaaatgatg
     1621 gctgtgaata cagccggggg gacaaagcgc agcgagcgca gcaaccagat ggggatgtta
     1681 atcttacccc acagcattat gaaagaaagg cgggtgaggt aggtaagcaa gcccgcagcc
     1741 agcatgacca gccacatggt cattttctgc tctccaacca catgccagtg attatgccca
     1801 ccagagcggc gcaaaccaag cccagtttaa agggtagcgg gtgcgccagg actgccgtga
     1861 ccccagccgc cagagcggcc gccaggctag ggcggtctct caagttaggc accaataggg
     1921 cgatgaatgt gagcaccagg gagtagtcca gcgaccagct ggcgggaacc tgggcgccta
     1981 tgaagacgcc catcgctgtg ctcacttgcc aagtagacca caaacccaac cctgcaccca
     2041 gcaagtacca gtgcttattc cccgtggact ctgggttact gtgactgtca tggctttgat
     2101 agtggtgaat gctcaccgcg taagcttcat cggtgagcaa gtaagccagg gtccacttcc
     2161 aggcgggtct cagcttgcgg gtgtatggtg cgattgaggc gctatacagt gcgtgccgaa
     2221 ggttgacgat ggctgccgtc agcaccatga ccatggcagg cgtgccgtgc ccgaacaatt
     2281 gggcggtgat gaactgcgaa gagcccgcaa acaggatgag tgacattgcc tgcgcctgca
     2341 ccgccgggat gccagccgag acagccaaaa tgccatagat cattccaaag gggattacac
     2401 ccaccagaag cggtagctcg tctctgactc ccgagagaaa ttccgtccga ggcgatgtca
     2461 tttctcactc cagatagggt aagggatgag ggatgtgggg atgagagacg aagtaccttt
     2521 ttgcacttaa acctaactgg gattcggatg ggattggaca gatcctgaag gctactcaag
     2581 ccttttggat ttgatctttc tcacggctga attgagtttc gtataggctg gcgtacaagc
     2641 cgttcagggc aagcaactcg gtatgggtgc cgcgctccac gaagcgaccc ctatccatca
     2701 ccaggatcag atccgctgcc aggatcgtgc tgaggcggtg ggcgatgaca atactggtgc
     2761 gccctgacat gacgcgcttc agggcttcct ggatgagcaa ttccgattcg ctgtccagat
     2821 ggctggtagc ctcgtcgagc acgaggatgc gcgggtcttt caatatcact cgcgccagtg
     2881 caatgcgctg tttctcgccg ccgctcaaac gataaccacg ctctccggca atggtatcgt
     2941 aaccatccgg cagcccagcg ataaaatcat ggatattggc cgccctggcc gcggcttcta
     3001 attcgtcctg ggtcgcctca aggcgagcgt agagcaggtt ggtgcggatg gtgtcgtgga
     3061 agagatgggt ctcttgggtg accataccga tctgggcgga gagcgagtcc agggatacct
     3121 ggcgcagatc atgcccatcg atcaggatgc gcccttcggt ggggtcgtaa aggcgaggga
     3181 tcaagtaggt gagggtggtc ttgcctgctc cgctggggcc caccagcgcc acgagttgtc
     3241 cgggttccac gcgaaagctg atcttgtcca gtgcaatcac gcgcgcctgg ctgcgcggta
     3301 cgtcttcatt cccttcatcg tcgcgagggt ggtgctccgg cctctcgccg gaaagaacgg
     3361 ttgtcacgct atccatttgc ccgaagcggc gcaccgcgct gagctctacc tgctcatcca
     3421 cttcataacg gaagctcact tcttcaaaga tcaattcacc ggaaatgttg gataagacac
     3481 gcgcctccga cttttccacg atatccatgg gtaggtcgat gacctcaaag acgcgctcaa
     3541 agctaacgat tgaggtggca aactcaaccg gtgcgttggc cagaccttgc aaggcgctgt
     3601 agaggctggt taggtatgcg ccgaaggcca cgatggtacc gatggtgaaa tccccttgga
     3661 tcaccaagta acccccgata ccgtatacca gggctgtacc catggcagag atcaggccaa
     3721 cgatgacaaa gaaggaagcc cctagcgagg cacgttggac gccggtatcg cgaaccttgg
     3781 atgcacgcag ttcaaagcgt gacacctcca ccggagcgcg tccaaagagc ttgaccagca
     3841 atgctccacc gatattaagc gtctcgttca tcatggcatt catctgggca ttaaaatcca
     3901 ttgcctggcg tgcgatatca cgcaggcgcg tccctaaccg gcgcgctgcc aggataaaaa
     3961 gcggcaaaat ggctatgctt accagtgtca gtcgccattc cagcgcaagc atcactgcca
     4021 gcacggcgga tgtctgcacc aagttggtga tggcagccac gatagtattg ctgatggcgt
     4081 tctgcgcgcc gacgacatcg ttgttcagcc ggctcatcag ctcgccgact ttggtgtggg
     4141 taaagaagcg cagcgacatg cgctgcaagt gggcgtagag cgctacacgc agatcgtaga
     4201 tcaccccctc gccgatcata gcgttcaagc ggcgttggat cacgttcaac ccgcccccca
     4261 gggcagggat gaacaataaa cctgaggcta gcagcatcaa ctggtttata tctctggcgg
     4321 gcagggcttt atcgattaaa gcgcgtaaga tcaatgggct gagcagcgtc agactggtgt
     4381 tcaccaggat gagggatagc atgccagcga tttgaccgcg gtaggggcga gcatagcgca
     4441 gcacacgccg cagcaaatcc caggtaacgc gcggcttttc accggtcgaa ctgatatagc
     4501 tccaccatcc agaaccatga aacatcaaga ctcctccaaa acaactgccc ctaaaaattt
     4561 acaccatata tggcaatttt tcaatggtat atagggatag atgggaactt tcttcatttg
     4621 tggatgtata ctggatacaa ggctcagaat cggcttgttt ttttgaacag cctcggttaa
     4681 ttaaccaatg gtgctagttt gaaaaagtca cgaggtacac tgctccaaaa gtccatcaga
     4741 ctcaagaagt gtgccctcat gaccaccgaa gattttatca ttgacttgtt ttgccgcgtg
     4801 gatgatcaaa tgcccgatac caagaagcat ttccaagccc atcgtggcat ggccgtacca
     4861 ggtaaattgg gttgtcatag gttcctcctt acaataagac aaaggggaag gctggcttga
     4921 agttccctcc attggtaaag gcctattcag cggatagtat attactgaag gtcatcctcc
     4981 atcaaccata gttgtggaaa cccgacattt tgcctaaagg taatgcgcca tttggctctt
     5041 gtatgggtga tttaaagctc ataaaataac gatactggtt gctgcgcagg cacaacttcg
     5101 ccggttaatc gcaatcgaag tcaacgtaga ccagcaggtg agtaccaagt gaatccgatt
     5161 ctggtaaaaa tggtttatcg tcctgccata aagcgtgcgg cctatgcagg tctggttgga
     5221 aggaatagag actgccagag gctggataaa ggccggttca ctcgtgccga agtagactac
     5281 attatggaac aggtttggca aggttacgac caacttgcac cagctatccc acgagaaccc
     5341 aaacttggta ataggatgaa tatgctcctc gcttgtgtga ccctttcctg ctttcaggca
     5401 ctgacatcag ctggtattga aagagattac gctatagaac tgattggaga cgtggcgtgg
     5461 aaagtatacg agaagtgggg acgactcact atatttgttg ctagattatt atcacgccac
     5521 ccccgggagc gaatgcgtat gtctgtgaat atgttcttgc gttttccatt tacaccccct
     5581 ggatatgtct tcgagcgttt agatagtagc gatggggtcg cgtttaatat gctcaggtgt
     5641 cccatatccg aatattttcg ggctcaaggt gcgtccgacc tttgtgtagg ctcatggtgc
     5701 aacttggatt tctcgcttgc ggaaatgtgg ggtggttggc tcgaacgaaa agaaactctg
     5761 gcagcaggat gtacgcgctg tgattttcgt ttcaagacag tggctcaagt ttgatggtca
     5821 taaccaatga ataaattaga gcgagagaaa accactttta gatgatttct agtcgatgca
     5881 cgacggaggt aataagatga ctgctcaaaa agtccgtgtg gctgttaatg gctacggagt
     5941 gatcggcaaa cgcatcgccg atgcagttgt agcccaggac gatatggaat tggtcggtgt
     6001 ggctgacgtg gtcagcgact ggcgcatcag agtcgccgtc gaaaaaggtt acccggtgtt
     6061 cgcctccacg ccgcaggctg ccgaagggat gcgtacggca ggtatactag tggctggtgt
     6121 tttggacgac ctggtatctc agatagatat agtagctgat gccacaccca aaaaggtggc
     6181 ttcggccaat ctggaaaaat accgtgctat cggggttaaa tccgtctttc aaggcggcga
     6241 aaagcatagc ctcactggac actcatttgt agcacaggca aactacgaaa ctgcaatagg
     6301 ccgggatacg acaagagtgg tttcgtgtaa tacgaccagc attgtgcgca ctttgggcgc
     6361 cctaaaaaca gccgggctgt tgaagaaagc gcgcggtgtg ctagttcgac gcgccaccga
     6421 cccgtgggaa tccgatcacg gcggtgtcat gaataccgtc gttccagaga cgatcatccc
     6481 ctctcatcag ggaccggacg ctcaaacggt cgatcctgac ctggacgtgg tgacgattgc
     6541 cctggtagcg gcccacacga ccagccacct gcattcttgg agcgtggagc taacccggcc
     6601 ggtcacgaag gaggaggtgc tggacgcgtt tcgcgcggcc ccacgcatcg ccttcctgcg
     6661 cgccggtgag ggcatcgtgg cgctcaacag cacgctggag atgatggccg accttgggag
     6721 accacggggc gatatgtggg aagtgggctt gtgggaggac agcctgacgg tgaaagggga
     6781 tgaagttttc tacaactacc aggtttacaa tcaagccatc gtcgtcccgg agacgatcga
     6841 cgccatccgg gcgataaccg gcatcgaaaa agacggcgcg gcgtccatcg ccaaaaccga
     6901 ccgggcgatg ggcctggtgc gcgagttcgt gcgacgaacg gacaggcttg ctgtgtgaga
     6961 tgaagaacca gctcacgatt ggtaagttgc gcggcctgca gcagatttcg gatgcgcgtg
     7021 gtatcttcgc catgtgcgcc atggaccacc gcggctcgat gcaacgcatg attagcaagg
     7081 ccgaatctaa aaaagtcgcc tatgagacat tggcggaata caagcgtgac cttgccgagt
     7141 acctggctcc tgagtctaca gggatcctgc tcgatccaat ctacggggcg gcgcagtcta
     7201 ttgcctttgg tgcgctgccc gggggtgtcg gcttgttggt cagcctggaa gaaactggct
     7261 acgagagcga cgctcaaggc cgggtgacca cgctgctgcc ggactggagc gtggagaaaa
     7321 tcaagcgcat gggcgcttct gcggtcaaga tgctgctata ctatcgcccc gacctgaccg
     7381 agaatgccgc ccgccagcgc gaagtcactc gtcaggtcag cgccgaatgt ttgtcttggg
     7441 acattccgtt cttgatcgag ccaatcgcct atgcggtctc tgaagctgaa ctagatccat
     7501 ccacctttgc cgcccggaag acgagcctgg tagtcgattc ggcgcgtgac ctgacgcaac
     7561 tgggcgtaga tgtgctgaaa gccgaatttc ctgccgacat gcgcttcgag cgggatgagg
     7621 acaagctgtg ggcagcctgt gaactgctcg atgcaagctc ggtatcgccg tggattttgt
     7681 tgagcgccgg ggtaagcttc gacgagttcg cccggcaggt gaagatcgcc tgccaagctg
     7741 gggcttcggg cttcctgggc ggccgcgcca tttgggaaga agcaatgcat atcaccgacc
     7801 ggggagaacg ccgtcaatgg ctggttactg tagggcgcga tcgcatgcgc cgcttgggcg
     7861 acatcgccgg tgaatacggc cggccgtggt ggaagaaatg ggctggctca atgagcgagc
     7921 tgatggatgt ctcacccgag tggtatcgcc gttattgatg gctacggcta tggcgaggtg
     7981 agatgaaggt tcgctcgtgg tatggcctca ccggcgagat agaggtcgag aacgagctct
     8041 ggcacctggt cagagtcggg ggagtggctt tgaaacaccc tcctatcatt aacttgctcc
     8101 tacggcgagg cctacctcgc gaggaccgcc tgtaccttag ctttctgcac gagtttggtc
     8161 acctgcagac cctcctggtc gctctgattc atggcactct aatgcttttt gcaaggcgta
     8221 ggcacggcag aggaagcttg agatcattaa cttggaaatg gcaagctctc atcgctcacc
     8281 aggctgtatg ggaattggca gccgagtctt acacggcgta ctatgccaga cctaggtatc
     8341 gccagattta tcggcggtat cccaatcccc ttggtcggtc tattttttgg attgggatgt
     8401 taggtttggc agtatatttt acacaacgcg cagtgaattc ttgaaggcaa tactctctca
     8461 taagagtggt cgttgataca agcaaattgg ctggggaaaa aacacgccaa acggcgctat
     8521 acactatagc aatcgaacct tatactctca ataattgaaa atgaaatatt tccattcaac
     8581 aggaggtcct cgtggataca caacatccaa gtaagttgtc tgacccattc tttagtttga
     8641 ttgtgtttag catctactcc gggttcatgg gagcggtgct tctgctcgca ccccggatca
     8701 tccttcctgc gtttcaggtg cacgaagagg tgaattcttt tacctatatg cttgggtttg
     8761 tcttgatagc ctcgtcgttc tactattttg cttccggcgt cgggagggac agattcttcg
     8821 ctaaacttac catatatacc cgaattgctt cccctattgt aacaatcatc ctgtacatta
     8881 ctgggaacgt gccggtcaat tatgtactcc tcagtatcct ggatgcctca ggcggagtct
     8941 ggacgtttta tgccctcaga aagacccgtt ctggggatag cctcaaaccg ctagggtatg
     9001 cacaacgggt ataatccatg ggggggcggg caatgaaagc aaatgccgcc aaagaatagc
     9061 tcggattacg tgccagggca tcactcagag cgagccgggt tgtaaagtaa cggcgctctt
     9121 agcagcctac gctggtcagc atcccattgt gctgaccagc attttgtatc atcttgttaa
     9181 accaatggtg ctagtttgaa aaaatcatga ggtacactgc tccaagtgtc catcagactc
     9241 aaggagtgtg ccctcatgac caccgaagat tttatcattg acctgttttg ccccgtgggt
     9301 gatcaaaggc ccgataccaa gaagcattcc caagccaatc attatcccag cgaagtggtc
     9361 acgcttgcca ttctgttcgc tctcaaaggg atgggcaatc gcgcctttta tcgctggctg
     9421 gagcgagatt acgggcattt gttccctgat ctgcccggcc gcatccgcct gtttcggctg
     9481 ttcaataccc gtcggcattg gacgcagcgc tttatggctg aaccttccct caatggtttg
     9541 atcgacacct atggcattga actgcttcac ccgcgccggg aaggccacag caggcggcaa
     9601 attggcaaga aagggctgtc caaccggcgt tggatcgtcg gaggcaagct gtgttttgtg
     9661 ctcaatcatc tgggtctgat cgtggattgg g
//
LOCUS       DBNGSX010000080         3126 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1598239, whole genome shotgun sequence.
ACCESSION   DBNGSX010000080 DBNGSX010000000
VERSION     DBNGSX010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1598239"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="AC16CB_02485"
     CDS             <1..298
                     /locus_tag="AC16CB_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341364.1"
                     /translation="YRLALIGYGGDLAEKAEWCDAQEQLELALSMGPDEQASRLLEDV
                     SNVCGGGDSGGSDNSNSNDNDNSQQPAETEAPPTEEQPGEPAATEPPPDSTPAP"
     gene            302..811
                     /locus_tag="AC16CB_02490"
     CDS             302..811
                     /locus_tag="AC16CB_02490"
                     /inference="COORDINATES: protein motif:HMM:NF017262.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HIR3341365.1"
                     /translation="MSSPPVWALSLIYWLHMLATVVWIGGLAALSFLVLPSAKRILEP
                     VAYSRLLDGLQRRLDPLSWLCLVVLVGTGLFQMSANPNYQGLLAISNRWSAAIFIKHL
                     VFLGMTATSAALSWWVLPQLRRAALRRTRQLDGTDEQRLHRREALLLRLNLMLAIVIL
                     ALTALARVS"
     gene            947..1156
                     /locus_tag="AC16CB_02495"
     CDS             947..1156
                     /locus_tag="AC16CB_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR3341366.1"
                     /translation="MDDRIVGIVKWFNSAKGYGFLGRDEGDDVFVHFSAIQMNGYRRL
                     EKDQKVEFSVEEGPKGLQAANVIPL"
     gene            complement(1258..2568)
                     /locus_tag="AC16CB_02500"
     CDS             complement(1258..2568)
                     /locus_tag="AC16CB_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341367.1"
                     /translation="MKRLPFILGGLILILLATGVRWSQPTSAAPALPFDELFIAPQGE
                     KPEQNWRVCRDLGVGNVPGLGIRRQRFRLCHSQGWEVLAYCLEPSLPAPAVGTRCSRT
                     NANTYWCGSGLQRLREYSVQETPTAAAPPSPVPTETALPTPTNSPPPPAAPPSAPPET
                     ATPERRAEATPERRARATQRVRAGGRGNFDLRSLLNLDWLAVPSKTPFQPQIATPTPF
                     QPHAPTAMIEATAQPSSSGFYGLDLADPGHRVRILIFPPNKKVNRGNPILISFIPGQT
                     CNYGDNRACVNTYQADTGASTTFITVHSGVGSEAQAYRHAIEGTGINSAGLSLKQVRA
                     NLQALDGAEVVIMQGKRRYEGFTLVAATRIPAKFMKAYFNAPIQESLAFASTIDDDLK
                     RLSDNNLPLLIFETCGWKMPGEAWARGVTSTTASVYLGVIQKKP"
     gene            complement(2565..3098)
                     /gene="hpt"
                     /locus_tag="AC16CB_02505"
     CDS             complement(2565..3098)
                     /gene="hpt"
                     /locus_tag="AC16CB_02505"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIR3341368.1"
                     /translation="MAQLPSDLARILIAEEAIQARVKELANEISKDLRGVESLYLVGI
                     LKGAFIFLADLARQLQIPHSVDFMALSSYGKSTTSGAVRILMDLREPIEGRHVMIVED
                     ILDTGHTLSYLYHILKGRNPASIRTCVLVRKRRESVEVPLDYLGFEIPDVWVVGYGLD
                     FADKYRTLPYIAELKQK"
BASE COUNT          673 a          835 c          925 g          693 t
ORIGIN      
        1 ctaccgtctg gccttgatcg gctacggcgg cgacctggct gaaaaagccg aatggtgtga
       61 tgctcaagag cagcttgaac tggcgctctc gatgggacca gatgagcagg cttcgagatt
      121 gctcgaagac gtgtcgaatg tctgtggcgg aggagatagc ggcggaagcg ataacagcaa
      181 tagcaatgac aacgacaaca gccaacaacc tgctgagaca gaagcaccgc caaccgaaga
      241 gcagccaggc gaaccggctg ccactgagcc tcctccagac agcacacccg cgccttaggc
      301 aatgagctcg ccaccagttt gggcgcttag cctgatctat tggctacata tgctcgctac
      361 cgtggtttgg attggcggac tggcagcgct ctcatttttg gtgttgcctt ctgccaagcg
      421 gatactggaa ccagttgcct acagtcgctt attagatggg ctgcagcgcc gcctcgaccc
      481 cttgagttgg ctctgcctgg ttgtattggt ggggacgggc ctgttccaaa tgagcgccaa
      541 tcccaactac cagggcttat tagcgattag caatcgctgg tcagctgcta tttttatcaa
      601 acatctggtt ttcctcggaa tgacagccac cagcgcggcg ttatcctggt gggtattgcc
      661 tcaacttcgc cgtgcagcct tacggcggac tcgccagctc gacggtaccg acgagcagcg
      721 tttgcacagg agggaggcgc tgttgctgcg tctcaacctg atgttggcga tcgtcatcct
      781 ggccttaacc gcccttgcca gggtatcata aatgtgtaag gattttccac ttgacatagt
      841 tttccaaaaa tgataatctt acgcccgttg tacgcagcag cctgactgtt attagaccca
      901 ggcgggtgac gctgtaaatc taatgtaaga gatggagaac gaaaaaatgg acgatcgtat
      961 tgttggaatt gtaaaatggt tcaactcagc gaaaggctac ggctttctcg gacgcgacga
     1021 gggcgatgat gtttttgtgc atttctctgc catccagatg aatggctacc gtcgcctgga
     1081 aaaagatcaa aaggtggaat tttcagtaga agaaggcccc aaaggtttgc aggctgctaa
     1141 cgtaattcct ctataataca tcaagacaat tgaataccaa cacaccccct tctcagtaga
     1201 agagggtgtt tttattttct ctgcttgtat tggatagggc aagcagggaa ctcttgctca
     1261 gggtttcttt tgaatcacac ccagataaac cgaggcggta gtgctggtga cccccctggc
     1321 ccaggcctca ccgggcatct tccagccaca ggtctcgaag atcagcagcg gcaggttgtt
     1381 atctgacaac cttttcaaat cgtcatcaat ggtcgaagcg aatgccagag attcctggat
     1441 gggcgcgttg aaataagcct tcatgaactt ggccgggatg cgcgtcgctg caaccagtgt
     1501 gaaaccctcg tacctgcgct tgccctgcat gatgacgacc tcagcgccat ccagcgcctg
     1561 caggttggcc cgcacctgct tgagagacag ccccgcgcta ttgatccctg tgccttcaat
     1621 ggcgtgtcgg taggcttgcg cctcactccc cacgccggaa tggacggtga tgaaggtcgt
     1681 cgatgcacct gtgtctgcct ggtaggtgtt gacacaggcg cggttgtcgc cgtagttgca
     1741 ggtctgcccc gggatgaacg agatcaagat cggattgccc cggttgacct ttttattggg
     1801 tgggaagatc aagatgcgca cgcgatggcc tggatcggca aggtccaggc cataaaaacc
     1861 gcttgaggat ggttgcgctg tggcttcgat catcgcagta ggagcgtgcg gttgaaatgg
     1921 ggtaggggtg gcgatctgag gctggaaggg ggtcttgctt ggaaccgcca gccagtccag
     1981 gttcagcagg ctacgcaggt cgaagttgcc cctcccgcca gcccggacgc gctgtgtagc
     2041 ccgtgcacga cgctccggtg tggcttcggc gcggcgctct ggtgtggcag tttcaggggg
     2101 agcactggga ggtgcagctg gcggaggagg ggaattggtg ggggtgggca atgcagtttc
     2161 ggtaggcacc ggggaagggg gcgcagctgc cgtgggcgtc tcttgcacag aatactcccg
     2221 cagtctctgc aggccactgc cgcaccaata ggtattggcg ttggtgcgcg agcagcgcgt
     2281 ccccaccgct ggggcaggca ggctgggctc gagacagtac gccagcacct cccaaccttg
     2341 gctgtggcac aagcggaaac gctggcggcg gatgcccaaa ccgggcacat tgccaacccc
     2401 caggtcacgg catacccgcc agttctgctc cggtttttcg ccctgggggg cgatgaacaa
     2461 ttcatcgaag ggcaaagcag gcgcggcgga tgtgggctgc gaccagcgca ccccggtcgc
     2521 tagcaaaatc agtatcagtc cccccaggat gaagggcaag cgcttcattt ttgctttagt
     2581 tcagcgatat atggcagggt tcgatattta tcggcgaaat ccagcccgta gccgaccacc
     2641 catacatcgg ggatctcaaa gcccagataa tctaggggca cttccacgct ttcacgccgt
     2701 ttgcgcacca gcacacaggt gcgaattgat gccgggtttc tacctttaag tatatggtac
     2761 agataactca aggtatggcc ggtatccagg atatcctcaa cgatcattac gtgtcgcccc
     2821 tcgattggct cgcgcaggtc catcaggatg cgtaccgctc cagaggtggt gctcttcccg
     2881 tagctggaga gcgccatgaa gtccacgcta tgagggattt ggagctggcg cgccaggtca
     2941 gccagaaaga taaatgcgcc tttgaggatg ccaaccaggt aaaggctctc caccccgcgc
     3001 aggtctttgg aaatctcgtt ggctaattct ttgacacggg cctggatggc ctcttctgcg
     3061 ataagaatgc gggctaagtc agagggcaat tgggccatgt gggcaactcc cagtttgaat
     3121 tatttg
//
LOCUS       DBNGSX010000081        33227 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_144334, whole genome shotgun sequence.
ACCESSION   DBNGSX010000081 DBNGSX010000000
VERSION     DBNGSX010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 33227)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 33227)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33227
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_144334"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1960)
                     /locus_tag="AC16CB_02510"
     CDS             complement(<1..1960)
                     /locus_tag="AC16CB_02510"
                     /inference="COORDINATES: protein motif:HMM:NF014647.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3341369.1"
                     /translation="MTKIFKFLTLIMVLAMLLSACKPAATPTEEAPPPVEATEPPAVE
                     PEAPKQFRVAVVMPSATNDLAFSQSMFDALNAIKNEMGAENFDFAYSEGIFVVDDAAA
                     ALRDYASQGYDLVIAHGSQYGSSLQEIAPDFPDTAFAWGTTVDTFVDQGINNVFAYEA
                     RSEEGGYVNGVIAASLSQSNILGVVGPIETGDAKLYVDGFVAGAKATKPDLQVNVNYI
                     GSFSDVALASEAAQTHINAGADGLTGTAQMVVGAIGVAEEKGVPWFGTQSNQTSLAPS
                     IVVANQVYDWTVALDEIISLAKSGTLGGKSYALTLANGGLKIEFNPDYAVPDDVKALA
                     DQTIAGIIDGSIVVGEAPPPVAAEEIAFGMVLVGPKGDRGWSQAHFTGGEYVEQNLPG
                     ARMMVFESFNPADKPEATLEGVVADMINDGAKLIFTTSDEFEEDTKTVAEGRPDVIFI
                     NISGDDVLTGEAPANEGNIMGRMEDMKAVAGCAAALATQTGRLGYLGPLINFETRRLA
                     ASAYLGARYCYENYRGMNPDDLKFTVTWIGFWFNIPGVTLDPTEVTNSFLDSGVDVVM
                     SGIDTTEGIDVSGQRAQQGEQVWAVPYDFEGACEQAPDICLGVPYFNWGPAYLETVKA
                     VQAGTWEQAWDWNPPAWDALTDNTKTAVG"
     gene            complement(2067..2999)
                     /locus_tag="AC16CB_02515"
     CDS             complement(2067..2999)
                     /locus_tag="AC16CB_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3341370.1"
                     /translation="MISDLLTMNVLVGILASGIRLATPYLYATIGEAFGQRSGVLNLG
                     LDGQMLLGALAAFYVAFNTGNLWLGLLAAVIVGAILGLAMAFVSVTLQAEQGISGIGF
                     YLFGLGMSDLLFQKTLGTVETVSGFPPIHIPFLSDIPILGRVFFSQNLMVYMAYLLVP
                     IAWFVLNKTTIGLKIRAVGENPDAADSLGVSVSRVRYLTVTLGGALSGVAGASLSIAL
                     LNVFQQNLTNGLGFIAVALVYFGGWRPVGVLAGSLLFSMVNALQLWVQVLGIPIPSDI
                     AIMMPYVLTILVLVFAGQRVRPPAALSKPFERGE"
     gene            complement(2996..4132)
                     /locus_tag="AC16CB_02520"
     CDS             complement(2996..4132)
                     /locus_tag="AC16CB_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3341371.1"
                     /translation="MKLLLGINPRWDKLANFFMPILAALAALAVGAVMLLLLGVNPLV
                     AYSALLEGAFGSQNALADTLVKATPLLLVGIGICIAFRGGVINIGGEGQLVVGAIAST
                     LVGLSFPEAPGWLIIPLALLAGFLSGAIWGGIPGVLKAYFNVNEILSTIMMNQIAVQG
                     MNFLLRGPMIDPVQLEAASKIPQTARLSRLFDLPRLVPTRLHLGSALAVIMAILIFIF
                     LWRTITGYRIRAVGLNPHASRYAGIHVQRYVVLALLLSGALGGLAGAVQVFGVHHRMF
                     TDGSATGFTGSAGFNGIVAALFGQLHPLGTIPASFLFGALIVGANAMQRAVQVPSALI
                     TALNGLLVVFVVSTDLWRRRLQRRQLSEAIREEAAQVKEVKAAS"
     gene            complement(4149..5126)
                     /locus_tag="AC16CB_02525"
     CDS             complement(4149..5126)
                     /locus_tag="AC16CB_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011432830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3341372.1"
                     /translation="MNEIEFVRTMASIILASSPLIIAVCGETITERSGVVNLSLDGTM
                     LMAAMTGFVVGLKTDNVWLGFLAGAIIGAIFGAIVAFSSISLGKSQVAVGFVLTLLGD
                     DLSAFLGQNFTRIPGALVTRFGIPFLEDIPFLGPILFKQDIVVYFAYALVIFTWWFLN
                     RTQPGLHLRSSGERPEAAFARGVNVRRVRYLYALVGGALVGVAGAAYSLDVKIGWSDG
                     HTRGLGWIALAIVIFGGWSPIRGALGAILFGATKALATILQRTFPEVSVVAFNAIPWV
                     LMIAVLLVVSSDVTERIVSVMPNRIQPPLRRMLRINAPLALGSSLAEKG"
     gene            complement(5123..6166)
                     /locus_tag="AC16CB_02530"
     CDS             complement(5123..6166)
                     /locus_tag="AC16CB_02530"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3341373.1"
                     /translation="MRLNRVASWFWPAVPVVAALLFTAGLLFLFQASPIESFKAMYDG
                     AFGDQSKTLSVLAFWVPITLASSGLLVTFTAGLWNIGVEGQIILGSIAASWIALKFKA
                     PTPFIQVTLEILAAMAGGALWAALTAVLKTRGGVNEIFGGLALNNLAIILTNYLISGP
                     WQPPEGGTFRGTQPFAPEALLPRYAESRFSPISMVITFVVIGLLIVMLRGTFWGLKLK
                     ALGKNAYSSFLLGVSSEREVILAMMLCGALAGLGGAVRVLSWFDSLRQSISGGIGFLA
                     LLVVMLANYHAYWVPFIAIFFSAVLNGSITLQLRTQLHSSLGGILTGVMVLFVLLFGD
                     VGVLRWFRKEKQE"
     gene            complement(6163..7629)
                     /locus_tag="AC16CB_02535"
     CDS             complement(6163..7629)
                     /locus_tag="AC16CB_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3341374.1"
                     /translation="MQIELKEIRKYFGNVKANDGISLVLQAGKIYGLLGENGAGKSTL
                     MKILSGYQPADSGSILLDGQSVHFTSPAQALSNGIGMLYQEPLDFPPFRIVDNYLLGR
                     DRKLMLDHKSAENILNNLASRYSFELDVHAFVDSLSMGERQQLELVRLLAGGAEVLIL
                     DEPTTGISAEQKDLLFDSMRKLAHEEGKTLILVSHKLDEVQELCDHAYVLRRGKLVGE
                     IDIPCPNEKLVEMMFAQIPPRTDRAANIIPGHPVVEMQDVTISTYRLSIEKINLAIQP
                     GEVFGLAGLEGSGQQLVLQACTGLLHPVHGHMRLAGQDVTNWSYHHMREAGVAYIAAG
                     RLEGGLVAGLSLTEHFILAQPEHTFFLDWSGAQKESASRIEKYQVVGTPLSLADELSG
                     GNQQRLLFALLRDDLKLVVMEHPTRGLDVRSSDWIWNILYERRKEGTAILFMSADLDE
                     IVERSDRIAVFSGGRMSRIVNAKETSVEELGHLIGGQE"
     gene            complement(7690..8652)
                     /gene="arcC"
                     /locus_tag="AC16CB_02540"
     CDS             complement(7690..8652)
                     /gene="arcC"
                     /locus_tag="AC16CB_02540"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HIR3341375.1"
                     /translation="MGNNGNKVAVVAIGGNSLIKDKNHQTVEDQYLAAKETTLHIADM
                     IEAGWDVAIGHGNGPQVGFILRRSEIAHKVEGMHEVPLDVCGADSQGAIGYALQQTLQ
                     NELYKRGIQKPVATVVTQVLVDKDDPAFRNPSKPIGGFMDEAEAQTRAKEMGWSVVED
                     AGRGWRRVVASPLPKEVVELESVNALIKHGVVVITVGGGGIPVIDAGDGEYRGTAAVI
                     DKDYASSLLAREINADLFLISTAVEKVGLNYGKPDQKWIDRMTLAEAKQYLADGTHFA
                     KGSMAPKVQAIIWYLEAGGKQAIITNPENIGRALRGETGTWIVP"
     gene            complement(8674..9654)
                     /locus_tag="AC16CB_02545"
     CDS             complement(8674..9654)
                     /locus_tag="AC16CB_02545"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005543 - phospholipid binding [Evidence
                     IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0006593 - L-ornithine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR3341376.1"
                     /translation="MQTDLRNRDLIGDLDFSREEVETVMEVAFDLKRKRALGESHAYL
                     RDKVLAMLFFFSSTRTRGSFEAGMAQLGGHAAFIESKTTQIAHGDTPKEIGEIFGRYF
                     DGIAIRHVDWGVGNGYLNAVAKASRVPVLNMQCDIYHPFQCLADLMTIFEKKGRDLRR
                     KRMVVSWAYAASYQKPMSVPQSLVLQMPRFGMDVILAHPPEFKLMPDIMDQAQEQARK
                     FGVGFEVMDDMEAAFKDADIIYAKSWGAMVHTPDPEEGGKIIKKYSHWITDERKMALA
                     KPDAIYMHPLPADRNIEVTDGVMDGPNSVVYDEAENRMHAQKAVMALTMR"
     gene            complement(9669..10619)
                     /gene="pyrB"
                     /locus_tag="AC16CB_02550"
     CDS             complement(9669..10619)
                     /gene="pyrB"
                     /locus_tag="AC16CB_02550"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HIR3341377.1"
                     /translation="MRSFNGRDILSLKGFEREEYFRVFEVADELSPFARNRRNTDLLK
                     EKTLVTAFYQPSTRTRLAHEAAMHRLGGHVTGFSDAKMTRAGDFYQESIKDTVKMLEF
                     YGDAIVMRHFQQGAPHEAARWASIPVINAGDGWGEHPTQVLTDLYTIQEEFGTLDGLN
                     FLLIGDMRMRTMHSISYAMTQFDINAFYVSPPEMSLTEDFKKELVDLNLNFKEIEHVE
                     QAIAEADVIYMEPVVQPDYTKSRDERTGEVGGTPANYKVTKELLRKAKQSAIVLHSLP
                     RMDELPTEVDDTRHERYWIEAFNGVVVRMALLALVLGAME"
     gene            complement(10697..11416)
                     /locus_tag="AC16CB_02555"
     CDS             complement(10697..11416)
                     /locus_tag="AC16CB_02555"
                     /inference="COORDINATES: protein motif:HMM:NF019322.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3341378.1"
                     /translation="MIHTSNTSQRLHESLGEIIAGTHNGGRLPSEPELARRLGVSRAT
                     LREAMRTFETQGLIRRRQGSGTYVVHPTHVLESGLEMLESIETMAERIGLRVSMGELK
                     IQHRPATAEEARNLALPASNMVMQLTRVMKAEGRPAAYLIDIIPDDVLPISELEEQFT
                     GSVLDLIIRRNEPELLTSRCEINADTASAEVARSLGIQRGDVLLRFVAYLYDSSGRVV
                     DYSISYFLPGYFRFHVVRRVG"
     gene            complement(11413..13227)
                     /gene="ade"
                     /locus_tag="AC16CB_02560"
     CDS             complement(11413..13227)
                     /gene="ade"
                     /locus_tag="AC16CB_02560"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HIR3341379.1"
                     /translation="MKKSPFNRVDTRALVDVAMGRVPADLVIRNGRWVCVQSGEIIPN
                     TDIAVKGERIAYVGPDAGHTIGKETKIIDAHGQYLVPGLLDGHMHVESGMLTVTEFVR
                     AVIPHGTTGMFVDPHEIANVFGLKGVRLMVDEAVVQPIHVWVQMPSCVPSAPGLETPG
                     ASIGPEEVAEAMTWEGIIGLGEMMNFPGVFFSDEKMHAEMAETRQAGKVIGGHYASPD
                     LGVPFHGYVAGGPEDDHEGTRLEDAVARVRQGMKPMLRFGSAWHDVASQVRAITEMGL
                     DARHFILCTDDSHSETLVKEGHVNRAVKIAIANGVPPITAIQMSTLNTAEHFGLARDV
                     GLIAPGRFADVLLVKDLPEMVPSQVISRGKLIAENGQLLIDLPAYPYPEWAVHSVRQA
                     HPLAAEDFRLSLPDGFSSNLKTVSANVIGVIENQAPTRHLKIDVPALEGEVNVDISRD
                     LAKIALVERHEGSGRVQVALVHGFGFDTSCAIGSTVAHDSHHMIVVGTDEADMAMAAN
                     ELAKAGGGQVVVKEGKIIGMVELPIAGLMSNEQADVVAEKALTVLEGFRTCGCKLNNP
                     NMQLSLLALVVIPELRISDLGLVDVTRFEFVPVFEERE"
     gene            complement(13276..14667)
                     /locus_tag="AC16CB_02565"
     CDS             complement(13276..14667)
                     /locus_tag="AC16CB_02565"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HIR3341380.1"
                     /translation="MTTILIQHANTLVTMDDHRREIPDGGLFIRDGFIEQVGETSQLP
                     ETADDILNLRDHIVLPGLVNTHHHFYQTLTRVIPAAQNANLFNWLKTLYPIWAKLTPD
                     DIYVSTQTALAELALSGCTTASDHLYIFPNGSRLDDEIHAAQEIGLRLHASRGSMSLG
                     ESKGGLPPDSVVEEEDFILRDSIRLIETYHDSKPGAMTQIVLAPCSPFSVTGDLMRQS
                     AVLARQYGVQLHTHLAETQDEEAFCQEMFGYRPVGYMQHLDWVGMDVWFAHSVHVSQE
                     EVGLYSRTGCGVAHCPTSNMRLASGFAPIYEMLEARVKVGLGVDGSASNDGSHLLEEV
                     RQAMLMARLRAALGGASLSSEGAPPILTARQALELGTRGGAAVLGRRDLGSLEPGKCA
                     DFFAINLNRLDYAGALHDPVAAVVFCTPQHVDYTMVGGKLVVKEGQLATLDLPMLVER
                     HNQSAHRLVYKPD"
     gene            complement(14891..16144)
                     /locus_tag="AC16CB_02570"
     CDS             complement(14891..16144)
                     /locus_tag="AC16CB_02570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3341381.1"
                     /translation="MISYHTCPLATLGGKDTGGMNVYVRDLTRQLGKMGIHVDVFTRS
                     QDEHVPHVLHDLGYGNRVVHIPAGPEVPLPKEVLASHLPEFVEGILAFAESKGLQYDL
                     IHSHYWMSGLAARDLKAEWNVPIVHMFHTLGIMKQRVARVPDEAEGDYRLKGESDVLQ
                     LADRIVASTLAELAQLQWLYKANVRKVVIIPPGVDLTHFYPIPTDEAKEYIGVLPCEQ
                     VVLFVGRIEPLKGIDTLIEAIAILRQRNVTVCLSVIGGDADVSQDELSDEMARLQVLR
                     EKYDLQDLVTFLGRRGQDTLPYYYSASEAVVVPSHYESFGMVALEAMACGTPVVASQV
                     GGLAYLVQDGVTGFTVPVDEPQALADCLIRLLQDASLRKRMGEQAAAFALDYDWDKIA
                     ARIVKLYAEISREDSRLSEADGTPH"
     gene            16294..16366
                     /locus_tag="AC16CB_02575"
     tRNA            16294..16366
                     /locus_tag="AC16CB_02575"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16327..16329,aa:Thr,seq:cgt)
     gene            16442..17083
                     /gene="udk"
                     /locus_tag="AC16CB_02580"
     CDS             16442..17083
                     /gene="udk"
                     /locus_tag="AC16CB_02580"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010688926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HIR3341382.1"
                     /translation="MQNRATPVVIGIAGGTGSGKTTVANVIMDRVGARHIAYLPHDAY
                     YKDLSALPEAQRVMVNFDHPNSLDTELLIFHIRQLRDWQTVDLPVYDFKTHSRTKEII
                     QIKPQPVILVEGILIFAEAALRPLFDVKIFVDTDADIRFIRRLQRDIEERGRTTHSVI
                     QQYLNTVRPMHLEFVEPSKRYADVIIPEGGLNTVAMDMVVARLEALLRGNNIG"
     gene            17080..17601
                     /locus_tag="AC16CB_02585"
     CDS             17080..17601
                     /locus_tag="AC16CB_02585"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HIR3341383.1"
                     /translation="MTNLRINIARVVAALLVIAISVTIYSIRDRADQFAVYGYPGIFF
                     LAFLAYATVLLPAPGLAVIFTMGAVFNPVGVALAAGSGAALGELTGYMAGFSGQAIVE
                     RTKIYARIAGWMKYNGPLTILFLAALPNPFFDIVGAIAGISKMPLLKFLFWCWIGETL
                     KMLFFALIGSGIL"
     gene            17622..18989
                     /locus_tag="AC16CB_02590"
     CDS             17622..18989
                     /locus_tag="AC16CB_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3341384.1"
                     /translation="MKNFEGIDTYLESNLEQSIQELSLLAAQPSISAQNLGLEECAHL
                     VGDLLKKRGFTVEIMQTGGPPVVYAERRGQSDKTLLFYNHYDVQPPEPLELWESPPFE
                     PSLRDGKLYARGVSDDKGHITSRLFALDALIDSNGDLPCNVKFVIEGEEETSSVHLNK
                     FVKDNAEKLAADACVWEFGQVDHREVPIQHLGLRGICYVELSVETAEQDVHSGLGGSI
                     FPNAAWRLVWALHTIKGPDERILIPGHYDNVVAPSEQDEQYIAELPEVADEYKNRYGV
                     QYFLHGLDGGVELRLAEVFQPTCTICGLTSGYQGPGSKTVLPAQASAKVDFRLVPNQT
                     PEQVLSGLRRHLDDQGFTDVKITYLGGESPARTDPNHSFVDMVVKTSQSVYDHPMQII
                     PITGGSGPIHAFIHYLKLPVVTAGLGYPGTRAHAPNENIRLDLYLKHAKHVARIMTAF
                     GDGTG"
     gene            19085..21796
                     /locus_tag="AC16CB_02595"
     CDS             19085..21796
                     /locus_tag="AC16CB_02595"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3341385.1"
                     /translation="MKLSLQRDLGLQLLALYLLFVGPVVVAALIFDRVASQRIESDVK
                     AADLALARAIAQETNTALGDALQSVENLASYQEVISMDKRGMEAIFTTLMSARPDVNL
                     VYRLDSQGLMVFHVPLGPGTTVGQDFSFRDYYQHALTARQPFLSQGRISPTTEQPVTT
                     AVTPIWDSHDNFLGLVATNIKLQSLSNTLSSIVAEHRPEEFFNVTIIDISGKVIAHPD
                     PQYPLTEAEFNLPEVTRAVLSGSSGNLIRPDPGGNNTLYSYVPIPSAGWGVIVSRPTA
                     AAFATSQATHRGVLLTIGVFLIVGLFFWLALSRQVLHPLERLAAFSRTIGLDEEFSED
                     HRLQLTTLSERPDQIGHLTRSLTRMEKAIEARLQELSTLLETSAAVVSTLDSNLVLNR
                     ILEQVERLMDIQMGAILALDEKRGVFRAQASRGLSKRYTENLAISPQEPISVTLRAIR
                     SGEPIQISDTEKDSSFTAMRPRARSEGYRSVLAVPLNIKHGPPSALLVYRPDPHIYTK
                     REINLLTSFANHAAMAIENAALYARSDMRLREQTRRLEALIQSLQDGLIMEDLQGIVL
                     HANRRIGEILELPLEEIIGVPGSQLIEHLLNHLASQDNAQRAKTTRLVEDALTGEGSR
                     RVELEIVTNHKLCYMVLRTFDVTDADGTPIGRGHILRDITRNREIDRMKSSLISTVSH
                     ELRTPLAAIKGYVTTLLAEDVMWDSLTQREFLEIISNESDRLSKLVNDLLDMSRIEAG
                     SLDVNRAQCNLEEIIQRAAQRAYPTPIERLVVNIPPDLPSLYVDPQRIEAVLRNLIEN
                     AAKYAGDISPVSISASVEGEEMVVRVRDEGPGIPAEHSNRIFESFYRLESNLTRMAHG
                     DGLGLAICQGFISAHGGKVWLEKQTKGTCVAFSLPLSQIPVEQLKAE"
     gene            21838..22527
                     /locus_tag="AC16CB_02600"
     CDS             21838..22527
                     /locus_tag="AC16CB_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3341386.1"
                     /translation="MVNITVLVVDDEKPLRDFVRRNLDVRGFNVRVASNGLEALAIFN
                     TQTIDLVILDVMMPRMDGLETIRRIRQSSLVPIIVLSALGEESDKIRALNLGADDYLT
                     KPFGVGELLARVQAVLRRARWAEPPTRQGRLARGEVVIDLERHETTVQGKEIELTPTE
                     FNLLVYLMENSGKVLPHQTILRHVWGPEYGPEHEYLRVYIGRLRQKIEDDPANPKYLI
                     TERGIGYSFEG"
     gene            22763..24100
                     /locus_tag="AC16CB_02605"
     CDS             22763..24100
                     /locus_tag="AC16CB_02605"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3341387.1"
                     /translation="MKHAFMTLLTLLIAATLLLSGCAPQAAPEEPAAPEAPAEPAATE
                     PPQEEPMGEEVTVVVGFTSSLTGSQEVSSKRQVNGFTLWINQINESGGITLSDGTVVK
                     FDFKTYDDESNAERVQELYTRLITEDNADFLISPYSSGLTSAASIVAEQNGKVMITAG
                     AADDAAYKTGNTSLFQLYTPGSLYMVSTVDMLKEIDPTATIALVHESDKFSTGVVDGI
                     KPYLESNGFEIVLEEGYASDTTDFGPIVNKIAGSGATVLLGGGHYNDGTALARALYDR
                     NIGLKFVYLLVAPADTKFPELGNAALGIATSSQWELAATHTEEEAANLGVEGFGPSGQ
                     EFATAYEAAYGDPPTYHVAGGYAAGLVLQKAIIDADSVEPEAVKAALNAMNLFTFYGG
                     IQFDTSEEAHGLQIAHKMVVAQWQKDDAGELKRVIIAPADVATAEPLYPIPAP"
     gene            24319..25185
                     /locus_tag="AC16CB_02610"
     CDS             24319..25185
                     /locus_tag="AC16CB_02610"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3341388.1"
                     /translation="MQQFSLLLTSLPDGILLGFVYGVAAMGLTLIWGVMNVINLSHGP
                     IIALGMFGTFFIFIRLGINPYIGLLLIAFLGLIIGVLIYFGAVHCVINTPHLSSLLAT
                     FSVNMIIIGLGTAALTTSPYNVDFNMGSFRIGLTTILGTRLIAALIAILFAGGLYLFL
                     YKTRPGKFIRAVANNRAAAELMGIPSTRILALSFGLGTMLAAVSGALIATFFPFSILS
                     GSGYELKSFVIGVLGGLGNPIGALLGGLILGVLEGIIPAFMETSWVPVIEFGLFVVIL
                     LVRPHGLFGIKK"
     gene            25182..26153
                     /locus_tag="AC16CB_02615"
     CDS             25182..26153
                     /locus_tag="AC16CB_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012941224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3341389.1"
                     /translation="MNKWRNHLGLLIPLLAVAAMVLWPIVTGNVSNREIMFTILKAIA
                     LASSLNILLGYTGYVSFGHIVFYGFGGYVGLYLVNEQGWSIWLGMLVGGLASGALAYL
                     LGKAILRLRGAYFALATIGINEAMKAFINNFEPFGGPIGMTLNFSVYKAYGGAAQALS
                     MSFYIMAFLTFATLIISYLVRTSKFGLGLRAIRENEDAAEVMGVVAPEAKTWAYVLSA
                     IIPGMVGVLFFFKNGNVEPYVAFPLHASIELLVMVMLGGQGTVLGPVLGALAYQGMRG
                     YLVTSTFFKDIQLSVSGVLLLLIVLFIPAGAIGWLRHRFSVLHKVLP"
     gene            26150..26896
                     /locus_tag="AC16CB_02620"
     CDS             26150..26896
                     /locus_tag="AC16CB_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012941225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3341390.1"
                     /translation="MILQVEGVSKRFGGLQALIRVTFDLPDGQILGLIGPNGAGKTTL
                     FNVINGVYPPNEGRVTFRGTDVTGMKPYQLAHLGLARSHQIVLPLNELSVRENVMVGA
                     CFGREKQSLGGAGKTAEEILDFVGLADRAEQLAGSLNIAQKKRLEMARALASRPHLLL
                     LDEVLAGLNPAEITTMVETIRRIRQQGVTIIMIEHVMHAVMNVSDRVIVLDYGEQIAE
                     GTPEEVSRNEKVIEAYLGDPKLAESLLKRN"
     gene            26910..27620
                     /locus_tag="AC16CB_02625"
     CDS             26910..27620
                     /locus_tag="AC16CB_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012941226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3341391.1"
                     /translation="MAIFEIRDLTSGYGEVQILWGATLSLDESKLTSLVGGNGAGKTT
                     MLRTIMGLTRPWEGSVWFEGKDISTLPPYMKAEMGLVLVPEGRQLFTDMSVAENLEMG
                     ASPKRARPHYKRNLERVYDLLPRLKERQHQKAGTLSGGEQQMLAVGRGIMAEPKILMI
                     DELSLGLAPVLVLSLFQTLLQLKDLGITLLLVEQNVQMALAVSNYGYVLSQGRVDLQG
                     PSEELAQNEHIRAAYLGL"
     gene            27641..28288
                     /locus_tag="AC16CB_02630"
     CDS             27641..28288
                     /locus_tag="AC16CB_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3341392.1"
                     /translation="MLVGERMNHPVITINPDMPVVDALKLMKRERIRRTPVIKDGKLV
                     GIVSDKDLLNASPSPATSLSVWELNYLLSKITVSEVMTRKVLIVSEDTPIEEAARIMA
                     DNKIGGLPVMRGNNLVGIITETDLFKIFLELMGARYMGVRATVLVHEERGQLAKLSQT
                     IANAGGNFVAFGMFQGEVPTNRLVTFKVDGIDKEQLKAVLEPIVESVVDIRDNSK"
     gene            complement(28393..29913)
                     /locus_tag="AC16CB_02635"
     CDS             complement(28393..29913)
                     /locus_tag="AC16CB_02635"
                     /inference="COORDINATES: protein motif:HMM:NF024948.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HIR3341393.1"
                     /translation="MNGTISPANQPTSEPRIKPQAWRTLSFFAAVTMELSWVALWIQL
                     LLATRIMISYWRAFLTCGAILLFSYYLDELNLRRNIHAAKRRVIILGVMLISVVFGLV
                     TLLNPGEWQSLKEFVLSPVQSFNDVAVSIPGEFLVIVASLMMSWRGLSLVGAMLEPES
                     IIGRFRTGIIMLFIFGLVVHLRETSPGFSFYLFIFSSLVGISAARIASQAHMRGGKTI
                     PFDRKWMAAILLTTSLVVGLAYWAVSWLRSGGLETIFTVYSWIISILVFLISPLLWAI
                     AELLRLLTEFISFEGVLQILIALFDRLGLFISELMEFVRSWIGNLDRLINLSWLQPLK
                     IPKSAVLWGTILFFVILILISLQKQRWKNIANEEEDLADIDQPDLFDLLRKGLRRAVD
                     QLINQVDSMLGLRQIQRRLAAARVRRIYAQLLKLSARLDSPRRSSQTPLEFLPSLRGL
                     FPDLMMELEAITKAYLHVRYGELPETANEVDQVERAWKLIQAEGQTKLREKRVVGQ"
     gene            complement(29964..31160)
                     /locus_tag="AC16CB_02640"
     CDS             complement(29964..31160)
                     /locus_tag="AC16CB_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR3341394.1"
                     /translation="MPIQRWLPLFIIIFYLGIVLRSPFLTAFSSTLLVIFGIAYWWHK
                     HALDGVRYTRRFRYTRGFPGEHFPVRLEIENNKLLPLSWLRVTDPWPKAVGPEDERAL
                     TPSHIADQGLLIHAFSLRWFERARRMYELYLRSRGVYKVGPVQVQSGDFFGIYKQSDR
                     LGSVDQITVFPTLIPLEELKLPPENPFGDQRSRRRFLEDPNFPMGVREYRPEDGFRRV
                     HWPATARTGQLQVKVFQPTSALTLVVCLNVLTYQHSWEGINPALFERLLSIAATLLYQ
                     GIEHGYRVGLISNGCLSNSDQPFHIPPGRSPKQLGRLLEALAGASPVMAKPFENFLMR
                     EIPHVPYGSTLVVVTALTSPALVETLSRLKKHERQITLFSLAEEPPPDILGVRCLHMP
                     FKEEEV"
     gene            complement(31162..32097)
                     /locus_tag="AC16CB_02645"
     CDS             complement(31162..32097)
                     /locus_tag="AC16CB_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3341395.1"
                     /translation="MIIDIAQRLRQNIQKVIIGKDEVSNLTLTVVLCEGHMLLEDVPG
                     IGKTTLARALAASLGCTFRRIQFTPDLLPSDVTGISWFNQKTQEFEFRSGPVISQVVL
                     ADEINRATPRTQSALLEAMQERQVTVDGVTRPLPRPFLVMATQNPIELEGTFPLPEAQ
                     IDRFLMRIALGYPEESEENAILERFRLADPLVDLEPVTTPQEILEYINQRRQVRIAEP
                     VREYIVRVARATRQNTEIELGASPRATLSLYQAAQAWAAIHGREYVLPDDVKNLAPYI
                     LSHRLMISPQAQLRGRKIEELISDIVEAVPVPVEG"
     gene            32282..>33227
                     /locus_tag="AC16CB_02650"
     CDS             32282..>33227
                     /locus_tag="AC16CB_02650"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR3341396.1"
                     /translation="MSSIVVLDIETTGLDSQKDAVIEIGAVRFNGRRVEDEWSTLINP
                     GRRIPPYITQLTGITDQMVLQAPAIKNVLQELVDFTGNSPVLGHNVGFDLSFLRRYNI
                     LLTNEPIDTYELASVLLPGARRYNLGALAQELSVPLRATHRALDDAHTTRGVFLRLID
                     LIMELPLNLLAEIVRLSEPLEWGGSWAFRQALRSRTKEIVNAQQVRHAYQGPIYTEAL
                     PRPSAPLHPLEKPVSLDIDDTAALLEHGGAFSHHFPQYEYRPQQVAMLRAVAEAISYS
                     RHLMVEAGTGTGKSMAYLIPATLWAIQNNNRVIISTNTI"
BASE COUNT         8350 a         9045 c         8066 g         7766 t
ORIGIN      
        1 atccaactgc agtcttggtg ttgtcggtta aggcatccca tgcaggcgga ttccaatccc
       61 aggcttgttc ccaggtgcct gcctggacag ccttgacagt ctccaggtaa gcaggacccc
      121 agttgaagta cggaacgcca aggcagatat ccggtgcttg ttcgcaggca ccttcgaagt
      181 cgtagggaac tgcccagacc tgctcacctt gctgagcgcg ctgcccagag acatcgatac
      241 cttcggtggt atcaatgccg ctcatcacga cgtcgacgcc agaatccaag aagctgttgg
      301 tgacttcggt cggatcgagg gtgacgccgg ggatattgaa ccagaagcca atccaggtca
      361 cagtgaactt caggtcatct gggttcatgc cccgatagtt ctcatagcaa tagcgcgcac
      421 ccaggtaggc ggaggctgcc aggcggcggg tttcaaaatt gatcaatgga ccaaggtaac
      481 cgagcctgcc agtttgggta gccagggctg ccgcacaacc tgcaacagct ttcatgtcct
      541 ccatacgacc cataatattg ccttcgttgg cgggggcttc accagtcaga acatcatctc
      601 ctgagatgtt aatgaagatg acatctgggc gaccttccgc tacggtcttg gtatcctcct
      661 cgaactcgtc cgaggttgtg aagatcaact tggcgccgtc attgatcatg tcggcgacca
      721 cgccctccag ggtagcctca ggcttatcgg ctgggttgaa ggattcaaag accatcatcc
      781 gtgcgccagg caagttctgc tcaacatatt ctccgccggt gaaatgcgcc tggctccaac
      841 cacggtcgcc cttggggcca acaaggacca tgccaaaggc aatctcttcc gcggcaaccg
      901 gaggcggggc ctcacccacc acgatggagc catcgataat cccggcgatg gtctgatcag
      961 ccagagcttt aacatcgtca ggcactgcat aatccgggtt aaactcgatc ttcaagccgc
     1021 cgtttgccag ggtcagggca taggatttac cacccaatgt cccgcttttc gccaggctga
     1081 tgatctcgtc tagggctact gtccagtcat acacctggtt ggcaactaca atgctaggag
     1141 ccagagaggt ctggttggat tgcgtgccaa accagggcac acccttctcc tccgccacac
     1201 cgattgcacc taccaccatc tgggcagtgc cggtcaggcc atctgcgcca gcgttgatgt
     1261 gggtttgggc agcttcagag gcaagggcca cgtcactgaa cgaaccaata tagttgacgt
     1321 ttacctggag atctggctta gtcgcctttg ccccagcaac gaagccatcc acgtacagct
     1381 tggcatcgcc ggtctcgata ggaccgacta ctccgagaat gttactctgt gacaggctgg
     1441 cagcgatcac gccattgacg taaccaccct cctcagagcg cgcctcatag gcgaacacat
     1501 tattgatacc ttgatctaca aaggtatcaa ccgtggtgcc ccaggcaaag gcagtatctg
     1561 ggaagtcggg ggcaatttcc tgcaacgatg agccatattg cgaaccatgg gcaatcacca
     1621 ggtcataacc ctggctagcg tagtcgcgca aggcagccgc agcgtcgtca actacaaata
     1681 tgccctcaga gtaggcaaag tcaaaattct ctgcccccat ctcattcttg atggcgttga
     1741 gggcatcgaa catgctctgg ctaaaggcca ggtcattggt ggcactgggc atcaccaccg
     1801 caaccctaaa ctgctttgga gcttcgggct caaccgctgg tggttcggtt gcttctacgg
     1861 gtggcggggc ttcttcagtc ggcgtcgcag ctggtttaca ggcgctgaga agcattgcca
     1921 gtaccattat tagggtgagg aatttgaata tcttagtcat ttctaccttc cttaaagtat
     1981 gagttagtct aaactgtcaa atatctctga tattgttgaa ggacaatctc tgaaattctt
     2041 ctctattcac ctccttggtg aaggatttat tctcctcgtt cgaatggttt ggacagcgca
     2101 gcgggaggac ggacccgctg cccggcaaat actaacacca atatggtgag cacatagggc
     2161 atcataattg cgatgtccga tgggattggg atgcccaata cctgcaccca gagctggagt
     2221 gcatttacca tgctgaagag cagcgaacct gccagcacac ccactggacg ccatccaccg
     2281 aaatacacca gcgcaaccgc aataaagccc aatccattgg tcaggttctg ctggaagaca
     2341 ttgaggagtg caatagacag ggatgcacct gccacacccg agagcgcacc tcctaaggtg
     2401 accgttagat agcgcacacg gctgacgctc acacccaaag agtctgcagc gtcgggattt
     2461 tcgccaaccg cccttatctt aagcccgata gttgtcttat tcaatacaaa ccaagcgatt
     2521 ggaaccagca ggtatgccat ataaaccatc aagttttggc tgaaaaatac ccgtccaagg
     2581 ataggtatgt cgctcaaaaa cggaatatgt atgggaggaa aaccactcac cgtttctaca
     2641 gttcccagag tcttctgaaa aagcagatcg ctcatgccca ggccaaatag ataaaagccg
     2701 atgccgctga tcccctgctc agcctggaga gttacgctga caaacgccat cgccaatccc
     2761 aggatagcac ccacgatcac ggcggcaagc agccccaacc acaggtttcc tgtattaaaa
     2821 gcgacataga atgcagccaa agcgcccaaa agcatttgcc catccaaacc gagattgagc
     2881 acgccgctgc gctgcccaaa agcctctcca atggttgcat acaggtaagg cgtggccagg
     2941 cgaatgcctg atgccaggat gccgacaagc acattcatag ttaataggtc agagatcatg
     3001 atgctgcctt cacttctttg acctgcgcgg cctcttcgcg tatcgcttca ctaagctgcc
     3061 ggcgctgcaa acggcgcctc caaagatcag tgctgaccac aaaaaccacc agcagtccgt
     3121 ttagcgctgt gatcaatgcc gaaggcacct gcacggcgcg ctgcatggca ttggctccca
     3181 caatgagtgc accgaagagg aacgaggctg ggattgtacc aagcgggtgt aattgcccaa
     3241 agagcgccgc cacaatccca ttaaatccag cactgccagt aaatccggtg gcagagccat
     3301 ctgtaaacat gcggtggtgg actccaaata cctgtaccgc gcctgccagc cccccgagcg
     3361 ccccactcag caacaaggcg agaacaacat accgctgcac atggataccc gcatagcgag
     3421 atgcatgggg attcagccct actgcgcgga ttctatagcc tgtaatggtg cgccagagaa
     3481 atatgaaaat gagaatcgcc atgatcactg ccaatgcaga tcctaagtga aggcgcgtgg
     3541 gcactaaacg aggaaggtcg aacaatctgg ataaccgcgc agtttgaggg attttcgatg
     3601 ctgcctcaag ttgaaccgga tcaatcatcg ggccgcgtaa taggaaattc atcccttgta
     3661 cagcgatttg gttcatcatg attgtactca ggatttcatt gacattaaaa tacgctttca
     3721 acacaccagg tatgccaccc catatcgctc cgctcaaaaa gcccgctaac agcgccaagg
     3781 ggatgataag ccagcctggt gcttcgggga aagataatcc caccagggtg gaggcaatcg
     3841 ccccaaccac cagttggccc tcaccgccaa tattgatcac gccgccccta aaagcgatac
     3901 aaatgccgat acccacaagc agcaagggtg tagctttcac cagggtatcc gctagagcat
     3961 tctggctgcc aaaggctcct tccagcagcg cagagtaggc cacaagaggg ttgacgccca
     4021 acaggagtaa catcaccgca cccacagcta gagctgccag agctgccagg atgggcataa
     4081 agaaattagc tagcttgtcc caacgcggat tgatacctaa aagtaatttc ataaaataac
     4141 gaattatcct atcctttttc agccagagag ctccccaggg ctagcggagc attgatacgc
     4201 aacatgcgcc tcagaggcgg ttggatccga ttaggcatca cgctaactat ccgctcggtc
     4261 acgtcactgc tgactacgag gagaactgcg atcatcaaca cccaggggat agcattgaaa
     4321 gccacaaccg agacctctgg gaaggtgcgc tgaaggatcg tagccagtgc ttttgtggca
     4381 ccgaacaaga ttgcgccaag cgcaccgcga atcggagacc agccgccaaa aataacaatc
     4441 gccagtgcaa tccagcccaa gccgcgggta tgcccatccg accagcctat tttaacgtct
     4501 aacgaatagg ctgcgcctgc tacacccacc agcgcgccgc cgaccagtgc atacagatag
     4561 cggacacgcc gcacattcac tccgcgagcg aaagctgcct caggtcgctc gccactactt
     4621 cttaagtgca acccgggttg ggtgcgattg agaaaccacc aggtgaagat aaccaacgca
     4681 taggcaaaat acacgacaat atcttgtttg aataagatcg gtccgagaaa agggatatct
     4741 tccaaaaagg ggataccaaa acgcgtgacc aatgcgcccg ggatgcgagt gaagttctgc
     4801 ccgagaaatg cgcttaagtc atcacctaga agtgtgagca caaagccaac tgccacctgg
     4861 ctctttccga gggagatgct gctgaatgcc acaatcgcac caaatattgc cccaataatt
     4921 gctcccgcca agaaaccaag ccagacgtta tcggttttta gcccaacaac aaatcccgtc
     4981 attgccgcca tgagcatcgt gccatccagg gataggttta ccacaccgga ccgctctgta
     5041 attgtctcgc cacaaacagc aataatcagg ggcgaagaag ctaaaatgat ggaggccatc
     5101 gtgcggacga attcgatctc attcattctt gcttctcctt gcgaaaccac ctaagtacac
     5161 cgacatcccc aaataacagg acaaacaaaa ccatcacacc ggtcaagata ccgcccaagg
     5221 atgagtgcaa ttgcgtacgc agttgcaatg tgatactgcc atttaagacc gcagagaaaa
     5281 aaatcgcaat aaagggtacc caataggcat gataattcgc caacatcacc accagcaagg
     5341 ccaaaaagcc aatgcctccg gagatgcttt ggcgaaggct gtcgaaccag ctgagcacac
     5401 ggacagctcc acccaagccg gctaatgccc cgcacagcat catcgccagg atgacttccc
     5461 gttcactgga cacccctaga aggaatgagg agtaggcatt tttacccaat gctttgagct
     5521 tcaaacccca aaacgtcccg cgcaacataa caatcagcaa accgatcacc acaaatgtga
     5581 tgaccatcga aatagggcta aagcgtgatt cagcgtatcg gggtagcagg gcctctggag
     5641 caaatggctg tgtaccccga aaagtacccc cctccggtgg ttgccagggg cctgagatga
     5701 ggtaatttgt caggataatc gccagattgt tcaaagccag cccaccgaag atctcgttca
     5761 ctcccccccg ggtttttaat actgcagtga gagccgccca gagcgcccct cccgccatgg
     5821 ctgccaggat ttccaatgtc acctggataa atggtgttgg cgctttgaat ttcagcgcaa
     5881 tccaacttgc tgcaatcgag cccaaaatga tctgtccttc gacaccgata ttccacaaac
     5941 ccgcagtgaa ggttaccaat aaaccggaag aggctaaggt aatcggcacc caaaatgcca
     6001 gcacgctcag ggttttcgac tggtcgccaa atgcgccatc atacatcgct ttaaatgatt
     6061 caatggggct ggcttggaat aagaacagca agcccgcagt aaagagcaat gcagccacta
     6121 caggcacggc gggccagaac cagcttgcca ctcgattcaa tctcattctt gtcccccgat
     6181 caaatgtccc aattcttcca cggaagtctc ctttgcattc acaatgcgtg acatcctccc
     6241 accagagaag accgcaatgc gatcgctgcg ctccacgatc tcatccagat cggcggacat
     6301 aaacaatatg gctgttcctt ctttccggcg ttcatataat atgttccaaa tccaatctga
     6361 agaacgaacg tcaagcccgc gggtgggatg ctccatgaca acaagcttta aatcatccct
     6421 caacaaggca aacaataagc gttgctggtt accacctgaa agttcatccg ccaatgagag
     6481 cggagtacct accacttgat atttttcaat tcgggaagca gattccttct gcgcacccga
     6541 ccagtccaag aaaaatgtgt gttcaggctg cgctaagatg aaatgctcgg ttaacgacag
     6601 accagcaact aatccccctt ctaagcgacc agccgctata taggctacac cggcttctcg
     6661 catatggtga taagaccaat tcgtcacatc ttgaccagca agacgcatat gcccatgcac
     6721 aggatgaagc agaccggtac aggcctggag cacgagttgc tgtccactgc cctccaagcc
     6781 cgccaatcca aacacctcac cgggttggat agccagattg attttctcaa tggataaacg
     6841 atatgtggag atcgtgacat cctgcatctc aacaactgga tgccctggga tgatatttgc
     6901 tgcacggtca gtgcgcggag ggatctgggc aaacatcatc tcgaccaatt tctcatttgg
     6961 gcatgggata tctatctcgc ccaccagctt gccccgcctc aagacatagg catgatcaca
     7021 tagctcttgg acttcatcaa gcttgtgtga taccagtatc agcgtcttac cttcttcatg
     7081 ggcaagcttt cgcattgagt cgaacagcaa atctttctgt tcggcgctga ttccggtagt
     7141 cggttcatct aaaatcaaga cctcagctcc ccccgctaag aggcgcacca attcaagctg
     7201 ctgtcgctct cccatggaga ggctatctac aaatgcatgc acatccaatt caaagctgta
     7261 gcgtgatgca aggttgttta aaatattttc tgcggatttg tgatccagca taagcttgcg
     7321 gtcacgaccc aggagataat tatccacgat gcggaaagga ggaaaatcta gcggctcctg
     7381 gtagagcatg cctatcccat tggataaagc ctgggctggc gaggtaaaat gcacactctg
     7441 accatccaat aagattgatc cactatcagc cggctggtag ccagataaaa tcttcatcaa
     7501 ggtgctctta ccagcaccat tttcccccaa taacccataa attttacctg cctgaaggac
     7561 aagactgata ccatcatttg ccttgacatt cccgaagtat ttgcggatct ctttgagttc
     7621 gatttgcatc gcgccagccc tccaggcagg gttttagttg cagatatttt atcctatgat
     7681 ttaataaatc tagggcacaa tccacgtgcc tgtttcacct cttaatgctc gcccgatatt
     7741 ctcgggattt gtgatgatgg cttgcttgcc gccggcctct aggtaccaga tgatcgcctg
     7801 taccttgggc gccatggagc ctttggcaaa gtgggtgcca tcagccagat actgcttagc
     7861 ctcagccagt gtcatgcggt caatccattt ctgatccggc ttcccgtagt tgagacctac
     7921 tttctcaacc gcggtggaga tgaggaaaag gtcggcgttg atctcgcgtg ccagcaagct
     7981 gctggcgtag tctttatcga tcactgcggc tgttccacga tattcgccat caccggcatc
     8041 gatcacaggt atgcctcctc cacccacagt aatcacaacc acaccgtgct tgatgagtgc
     8101 attgaccgat tccagctcga ccacttcctt tggtaatggc gacgcaacta cgcgccgcca
     8161 gccacgacca gcatcttcca caacgctcca gcccatctcc ttggcgcgcg tttgggcttc
     8221 agcctcatcc atgaatccgc cgatgggctt ggatgggttg cgaaaggccg gatcatcctt
     8281 atccaccagc acttgggtga ccaccgtagc cactggtttt tggataccgc gcttgtacaa
     8341 ctcgttctgc agggtctgtt gcaacgcata tccgatggcg ccctggctat ctgcaccgca
     8401 tacatccagc ggcacctcgt gcatgccctc aaccttatga gctatttccg agcggcgcag
     8461 gatgaagcct acttgcggcc cgttgccatg accgatggca acatcccaac ctgcttcaat
     8521 catgtcggcg atgtgtaatg tggtctcctt agccgccagg tattggtctt ccaccgtctg
     8581 gtggttttta tctttaatga gcgaattgcc accgatggca actacggcca ctttatttcc
     8641 attgtttccc atgtcgctgt tcctcctgcc taactatctc attgtcagcg ccatcaccgc
     8701 tttctgcgcg tgcatgcggt tttcggcttc gtcataaact acggagtttg gaccatccat
     8761 tacgccatcg gtgacctcaa tattgcgatc agcaggcagc ggatgcatat aaatagcgtc
     8821 tggcttagcc agagccattt ttcgctcatc tgtaatccag tgcgaatact ttttaatgat
     8881 cttgccgccc tcttccgggt cgggagtgtg aaccatagca ccccaagatt tagcgtagat
     8941 gatgtcggca tccttgaagg cagcttccat atcatccatg acctcaaaac cgacgccgaa
     9001 cttgcgcgcc tgctcttgag cctgatccat gatatccggc atcaatttga actccggggg
     9061 atgagccagg ataacatcca tgccgaagcg aggcatctgc aacaccagtg actgcggcac
     9121 cgacatgggt ttctgatacg aagcagcata cgcccacgat actaccatgc gcttgcggcg
     9181 cagatcgcgg ccttttttct caaatatcgt catcaagtcg gctaagcatt ggaatggatg
     9241 ataaatatcg cactgcatgt tgagcactgg cacgcgtgag gccttagcca ccgcattcag
     9301 gtaaccattg ccaacccccc aatccacatg gcggatagcg atgccatcaa aataccgccc
     9361 aaagatctcg ccgatctcct ttggggtatc gccgtgagca atctgggtgg tcttgctctc
     9421 gataaacgcg gcatgaccgc cgagctgagc catgccagct tcaaacgagc cacgcgtgcg
     9481 cgtgctggag aagaagaaca acatggccag cactttgtcg cgcaggtaag catgcgattc
     9541 gcccaacgcg cgcttgcgct tcaggtcgaa agccacttcc ataacggttt caacctcttc
     9601 gcggctgaaa tccagatcgc caatcagatc acgatttctt aagtctgttt gcatcttaca
     9661 cttcctgttt attccatcgc gccgagaacc aacgccagca gtgccatgcg caccaccacg
     9721 ccattaaacg cctcgatcca ataacgttcg tgacgggtgt cgtccacttc ggttggaagc
     9781 tcgtccatgc ggggcaagga atgtaacaca atggcactct gctttgcttt gcgcaataac
     9841 tcttttgtga ccttgtagtt cgccggggtc ccacccactt cgccggtgcg ctcatcacgc
     9901 gacttggtgt aatctggctg tacaaccggt tccatgtaaa tcacgtcagc ttccgcgata
     9961 gcctgttcaa catgttcaat ctctttgaag ttcaggttga ggtcgaccag ctccttcttg
    10021 aaatcttcag tcagactcat ctctggaggg gagacataga aagcgttgat gtcaaattgg
    10081 gtcatagcgt acgaaatgct gtgcatagtg cgcatgcgca tgtcgccgat gagcaagaaa
    10141 ttaagcccgt ccagcgtgcc aaactcttcc tggatggtgt acaggtcggt cagcacctgg
    10201 gtgggatgct ctccccagcc gtcccccgcg ttgatcaccg gaatgctggc ccagcgtgca
    10261 gcttcgtgcg gcgcgccttg ctgaaagtgg cgcatcacga tggcatcgcc gtaaaattcc
    10321 agcatcttta cggtatcttt gattgactcc tggtagaaat caccagcgcg ggtcattttt
    10381 gcatcagaga aacccgtcac atgaccacca aggcgatgca tggcggcttc atgtgccagc
    10441 cgcgtgcgcg tgctgggctg gtagaaagcc gtcaccaggg ttttttcctt gagcagatca
    10501 gtattacgcc gatttcgagc aaatggagac agctcgtcgg ctacttcgaa gacgcggaaa
    10561 tattcctctc tttcgaagcc tttgagtgac agaatgtcac gtccattgaa actacgcatc
    10621 ttactacctc cgagaactca ttcttaaaat tgagattcgt tgcgcatgat acatcgctgt
    10681 gcgtcatgcc acacggttaa cccacccggc gtacaacatg gaatcggaaa tatccgggta
    10741 ggaaataact aatagaataa tctacaacgc gaccagacga gtcgtacaaa taagccacaa
    10801 agcgcaggag cacatctcca cgctgaatac cgagagaacg cgccacctct gctgaggctg
    10861 tatcggcgtt gatctcgcat cgtgatgtga gcaactccgg ttcgttgcgg cgaatgatta
    10921 gatcaagcac cgagccagta aactgttctt caagttcaga gatcgggaga acatcatctg
    10981 ggataatatc aatcagatat gcagcaggcc taccttcggc tttcatgacc cgggtcagtt
    11041 gcatcaccat attgcttgcc ggcaaggcca ggttacgtgc ctcttcagct gttgccgggc
    11101 gatgctgaat ctttaactcc cccatggaaa cccgtaaacc gatgcgctcc gccatcgtct
    11161 cgatagattc cagcatctcc aacccgctct caagcacatg ggtaggatgc acaacatagg
    11221 taccagaacc ctgacggcgg cggatcaatc cttgggtctc aaaggtgcgc atggcctcgc
    11281 gaagagtggc tctggaaaca cccaagcgcc ttgctagttc gggctccgag gggagtctgc
    11341 caccgttatg ggtgccagca atgatctcac caaggctttc gtgcaatctt tgagaggtgt
    11401 tggaggtatg gatcattcgc gctcctcaaa aacggggaca aactcaaaac gagtcacatc
    11461 aaccagaccc aggtcagaga tacgcagttc gggtatcacc accagtgcta gtaggctgag
    11521 ctgcatattt gggttgttga gcttgcaacc gcaggtgcgg aaaccctcta aaactgttaa
    11581 tgctttttcg gcaacgacgt ccgcctgctc gttcgacatc aacccggcga tgggtaactc
    11641 aaccatgcct atgatcttgc cctcttttac aaccacttga ccacccccag ctttggccag
    11701 ctcattggct gccatagcca tatcggcctc atctgtgccc acaacaatca tatggtggct
    11761 atcatgggcg accgtggaac ctatcgcgca ggaggtatca aagccaaacc catgtacaag
    11821 ggcgacctgc accctccccg agccctcgtg gcgttccacc aaagcaattt tcgccaaatc
    11881 gcgcgagata tccacattaa cctcgccctc tagagcagga acatctatct tcaaatggcg
    11941 ggttggagct tggttttcaa ttactccaat cacattggcg ctcacagttt tgaggttgga
    12001 tgagaagcca tccggcaagg acaatcggaa gtcttctgca gccagcgggt gggcttgtcg
    12061 gacggaatga accgcccatt ctggatatgg gtatgcaggc aggtcgatca gcaattgacc
    12121 attctccgct attaacttcc cgcgtgagat gacctgggag ggcaccattt cagggagatc
    12181 cttaaccagc aatacatcag caaaccggcc gggagcgatc aatccgacat cccgcgccaa
    12241 cccgaagtgt tctgctgtat ttaaggtgct catctggatg gctgtaatcg ggggcacacc
    12301 attggcgata gcaatcttca cggcacgatt aacatgccct tcttttacca gtgtctcaga
    12361 atgcgagtcg tcagtacaca gtatgaagtg cctggcatcc aaacccatct cagtgatcgc
    12421 ccgaacctgg gaggctacat cgtgccatgc agatccgaag cgaagcatgg gcttcatgcc
    12481 ttggcgcaca cgggcaacag catcttccag gcgtgtgccc tcatggtcgt cctctgggcc
    12541 accagccaca tacccatgga aaggcacccc caggtctggc gaggcataat gccccccgat
    12601 cacctttccg gcttgacgcg tctctgccat ttcggcatgc attttctcat cgctgaagaa
    12661 aactccggga aagttcatca tctctcccaa gccgatgatt ccctcccagg tcatggcctc
    12721 tgccacctct tctggtccta tactcgcccc aggcgtctcc aggcccggag cggaggggac
    12781 gcaggaaggc atttgcaccc aaacgtggat aggttgcaca acggcttcat ccaccatcaa
    12841 ccgcacgcct ttcagaccaa agacgttggc gatttcatgg gggtccacga acataccggt
    12901 cgtgccatgc ggaatgaccg cccgcacaaa ttcagtgacg gtcagcatgc ctgattcaac
    12961 atgcatatgc ccatctaata atcctgggac aagatactgc ccgtgagcat cgatgatttt
    13021 ggtctccttg ccgattgtat gaccagcgtc aggtcctacg taagcgatcc gttcaccttt
    13081 cacagcaata tctgtattgg gaatgatctc accggattgc acacataccc aacgcccatt
    13141 tcggatcacc aggtcggctg gcactcgccc catggcaaca tccaccagcg ccctagtatc
    13201 aacgcgattg aaaggggatt ttttcatctt tcctccaatt ccggtaattg ctcgcttccc
    13261 aatatgcgct ggtaattaat caggcttgta aaccaacctg tgagcagact gattatgtct
    13321 ctccaccagc ataggcagat ccaatgtagc taactggccc tctttgacga ccagcttgcc
    13381 acctaccatg gtgtaatcta cgtgttgagg tgtgcaaaaa accacggctg cgaccggatc
    13441 gtgcagcgcg ccggcatagt ccaagcgatt gaggttaatg gcaaagaaat cggcgcattt
    13501 acccggttct aatgagccca aatcgcgcct gccaagcacg gcagctcctc cacgagtacc
    13561 gagctccaaa gcctgcctgg cggtcaatat tggcggtgcg ccttcgcttg aaagtgatgc
    13621 acctcctaac gcagctcgta aacgagccat taacatcgct tgccgaactt cctcaagcag
    13681 gtgggagcca tcgttactag cagagccatc tacacccaac cccaccttta cacgcgcttc
    13741 tagcatctca taaatgggcg cgaaaccaga agccagacgc atatttgagg ttgggcagtg
    13801 ggccacgccg cagccagtgc gtgagtacag acccacctct tcctggctaa catgaacaga
    13861 gtgggcaaac cagacatcca tcccaaccca atccaggtgc tgcatgtaac caacgggacg
    13921 gtaaccaaac atctcttggc aaaacgcctc ttcatcctgc gtctccgcca gatgagtatg
    13981 taattgaaca ccatactggc gcgccaggac tgcgctctgg cgcatcagat cacccgtgac
    14041 agagaatggc gagcaaggcg ccaaaacaat ttgggtcatc gcgccaggtt ttgaatcgtg
    14101 ataagtttcg atcaagcgga tagaatcgcg caggatgaaa tcctcttcct ccacgacaga
    14161 atcaggtggc aagcctccct tgctctcacc gaggctcatc gagccgcgtg aagcatgcag
    14221 gcgcaaacca atctcctgcg cagcatggat ttcatcatcc aggcgcgagc catttgggaa
    14281 aatgtacaag tgatcagagg ccgtagtgca acctgaaagc gccaactctg ccagagcagt
    14341 ttgggttgag acataaatat catcgggcgt caactttgcc caaatgggat atagggtctt
    14401 gagccagttg aacaggttgg cattttgcgc ggctggaatg acccttgtca gcgtttgata
    14461 aaagtggtga tgggtattga ccagacccgg tagaacgatg tgatcgcgta aattaaggat
    14521 atcatcggca gtctccggaa gctggcttgt ctctcccacc tgttcgatga aaccatcccg
    14581 aataaaaaga ccaccgtctg gtatctcacg gcgatggtca tccatagtca caagcgtatt
    14641 ggcgtgttga ataagaatag tagtcatcgt atgacccaag ccaacttgtc tgacaaattt
    14701 attttaacaa acaaaagcaa aatcgtcaat tgtctgacaa gtaatatttg taacttgagc
    14761 gattgccgaa aataatattt cccccctcgg ctatgctagc cgacaaatgg tctgaaaata
    14821 tccgagcaat ccgctatcgc gtctccagcg acccgcatat ggattatatg gcttgtttgg
    14881 aaatcaaaac ttaatgagga gttccgtcgg cttcagacaa tcgactatct tcccgtgaaa
    14941 tctccgcata gagcttgaca atcctcgctg caatcttatc ccaatcatag tcgagtgcaa
    15001 atgcagctgc ttgctccccc atacgcttgc gtaaagaggc atcctgcaac agccttatta
    15061 aacagtctgc aagtgcctgg ggctcatcta ctgggacggt gaatcctgtg accccatctt
    15121 gcaccaggta agcgagccca cccacctgcg aggccactac tggtgtaccg catgccatgg
    15181 cttccagggc aaccatgcca aacgattcat agtgagatgg gacaaccacc gcctcagaag
    15241 ccgagtaata atagggtagc gtatcttgcc cccgacgccc taaaaaagtg accaaatctt
    15301 gtaagtcgta tttctcgcga agaacttgca ggcgagccat ttcatcgctc aattcatctt
    15361 gactgacatc cgcatctcca ccgatcactg agaggcagac cgtcacattt cgctgtcgaa
    15421 ggatcgcaat cgcttcaatc aacgtgtcaa tacctttcaa tggctcgatc cgccccacaa
    15481 agagcactac ctgttcacaa ggcagcacac cgatgtactc tttagcctca tcggttggaa
    15541 tagggtaaaa atgggtcaaa tccacgcctg gtggaataat aaccaccttg cgcacattcg
    15601 ctttgtatag ccattgcagt tgagccaact ccgctaaagt agaagccaca attcgatccg
    15661 caagctgcag cacatcactt tcacccttca aacggtagtc cccttctgct tcatcaggca
    15721 cgcgcgccac ccgttgcttc attatgccaa gcgtgtgaaa catgtgcacg atgggaacat
    15781 tccactccgc cttcaggtcg cgggctgcca gccccgacat ccaataatgg ctgtgtatta
    15841 agtcgtactg caatcccttg ctttctgcga aagctagaat accttccaca aactcaggaa
    15901 gatgagatgc cagaacttct ttgggtagcg gtacctccgg acctgccggt atgtgtacca
    15961 cccggttacc ataacccagg tcgtgcaaaa catgcggcac gtgctcatcc tgcgaacgcg
    16021 taaagacatc cacatggata cccatctttc caagctgacg agtcaaatcg cgcacatata
    16081 cattcatacc gccagtgtct tttccaccca gggtggctag cggacaagta tgataagata
    16141 tcatggctat gcgcaactca cacctcgtct tacgcgactg ccttgcaatt cctacctatc
    16201 ccttgtataa tgtgccccgc atcccaaatt ttacgacccg cgcctcccaa acctaccagg
    16261 aggtcagcaa ccttgatggg ttcataatca ggtgccaccg tagctcagtt ggcagagcag
    16321 gcgtttcgta aacgtcaggt caagggttcg aatcccttcg gtggctctct ttaattggga
    16381 tgaatgagtg ggtatacctc tcattcatcc caaatttatt ccattccagt ataattccag
    16441 tatgcaaaat cgtgcaaccc cagttgtcat tggaatcgcg ggaggtactg gttccggtaa
    16501 aacaacggtg gccaatgtca tcatggatag ggttggcgca cgccatatcg cctacctgcc
    16561 gcatgatgcg tattataaag acctcagcgc acttcccgag gcgcaacgcg tcatggtgaa
    16621 ttttgatcac cccaactcat tggataccga attactcatc ttccatatcc gtcagcttag
    16681 ggactggcaa accgtcgatt tgccggttta cgattttaag acccacagcc gaaccaagga
    16741 aatcatccag atcaagcctc aaccggtcat actggtcgaa ggtatcctga tttttgccga
    16801 ggcagccctg cgcccgctct ttgatgtcaa gatttttgta gataccgatg ctgacatccg
    16861 gttcattcgc cgattgcaac gtgatattga ggagcgcggt cggactactc actcagttat
    16921 ccagcaatac cttaataccg tgcgccctat gcacctagaa ttcgtagaac catccaaacg
    16981 ttatgctgat gtgattatcc cagagggtgg attgaacacc gtggctatgg acatggtggt
    17041 ggcgcgcttg gaagccttat tgcggggtaa taacatcggg tgacgaatct tcgcatcaat
    17101 atcgcgcgcg tcgttgcagc actgctcgtc attgctatca gtgtcaccat atatagcatt
    17161 cgtgaccggg cagaccagtt cgctgtctat gggtatccgg gcatattttt tcttgccttt
    17221 ttggcatacg ctaccgtact actacccgct ccgggcttag cagtgatctt caccatgggc
    17281 gcggtgttta atcccgttgg tgtcgccctg gcagctggca gcggtgcagc attgggagag
    17341 ctaactggct acatggcagg ttttagtgga caggctatcg tcgagcgcac caagatttac
    17401 gcccggattg caggctggat gaaatataat ggtccactta caatcctttt tctggctgct
    17461 ttgcctaatc ccttttttga tattgtcggc gcgatcgccg gaatctcaaa aatgcccctg
    17521 ctaaaatttt tattctggtg ctggattggt gagacgctca aaatgttatt ttttgcccta
    17581 attggatcgg gcattctatg atctttcact aaggaaaaat gatgaaaaat tttgaaggaa
    17641 ttgacacata cttagaatca aatttagagc aaagcattca ggaattatca ttgctcgccg
    17701 cacaaccaag catcagtgcc caaaacctgg ggttggagga atgtgcccat ttggttgggg
    17761 atttactcaa aaagcgaggc tttacagtag agatcatgca gaccggaggc ccacctgtag
    17821 tttacgctga acggcgcggg caaagcgata aaacattgct gttctacaac cattacgatg
    17881 tccaaccccc tgagccgctc gagctttggg agagcccacc atttgaaccc agcttgcgtg
    17941 atgggaagct ctatgctcgc ggggttagcg atgataaagg tcacatcacc agccgtcttt
    18001 tcgcgctgga tgccttgata gactcaaatg gtgatctgcc ctgcaacgtc aaattcgtaa
    18061 tcgagggtga agaagagacc agcagcgtcc atctcaataa atttgtcaag gacaacgcag
    18121 aaaagctcgc cgcagatgct tgtgtatggg agttcgggca ggtggaccat cgcgaagtgc
    18181 caatccagca ccttggcctg cgcggaattt gctatgttga gttatctgtg gagacagcgg
    18241 agcaggatgt ccactcggga cttggcggat caattttccc gaatgcagcc tggaggctgg
    18301 tttgggcatt gcataccata aaagggccag acgagcgcat tctcatccct ggtcactacg
    18361 acaatgttgt tgcgcctagc gagcaagacg agcagtacat agcagaactc cctgaagtgg
    18421 cagacgaata caagaatcgt tatggagtac aatattttct acacgggctg gatggcggtg
    18481 ttgaattgcg cctggcagag gtatttcagc ctacttgcac catttgtgga ctgacgtctg
    18541 ggtaccaagg accaggcagt aaaactgtat taccggccca agcctcagcc aaggtggatt
    18601 tccgcttggt gccaaatcaa accccagagc aggtgttatc aggtttgcgc agacacttag
    18661 acgaccaagg atttaccgat gtgaagataa cctacctggg tggggagtcc cctgcgcgca
    18721 ccgacccgaa ccactccttt gtcgacatgg tagttaaaac ctcacaatct gtctacgatc
    18781 acccgatgca aatcatcccg atcaccggtg gatccgggcc aatccatgct tttatccatt
    18841 acctgaagct accggtagtt accgcaggtt tgggctaccc tggtactcga gcgcacgcgc
    18901 ccaacgagaa tatccggcta gatctttatc tcaaacacgc caagcatgta gcaagaatca
    18961 tgacagcgtt tggcgacggt actggatagt ttatgcgagg atatgagcgg atcccattac
    19021 gtgatcctca ccagcgaatt caataattca tgatattctt tttggaattc ggtgatagat
    19081 ttcgatgaaa ttatctctgc aacgtgacct gggattgcaa ttactggcgc tatatctctt
    19141 atttgtagga ccagtggttg tggctgcgtt aattttcgac cgcgtggctt ctcaacgtat
    19201 cgaatctgat gtcaaagcag cagacctcgc cctggcgcgc gctatcgccc aagagaccaa
    19261 taccgcttta ggtgacgctc tccagtcggt tgagaacctg gcctcatatc aagaagtaat
    19321 atcgatggac aaaagaggca tggaagccat ctttaccaca ctgatgagcg cccgccctga
    19381 tgtcaaccta gtataccgcc tggattcgca aggcttgatg gtctttcatg taccacttgg
    19441 gccaggcaca accgtaggcc aggatttctc gtttcgagat tattaccagc acgctttgac
    19501 agcacgccaa cccttcctct cccaagggcg tatatcaccc acaacagaac aacccgtaac
    19561 taccgccgtc acgccgattt gggactcaca tgataacttt ctcgggctgg tggcaaccaa
    19621 tattaagctg caatctctaa gcaacaccct atccagcatt gttgcagaac accggccgga
    19681 agaattcttc aacgtgacga ttatcgacat cagcggtaag gtgatcgcgc accccgaccc
    19741 acaataccca ctcaccgaag ccgaatttaa cctacccgag gtaacccggg cagtgctctc
    19801 cggctcttcc ggcaatctga tcaggccgga cccaggtgga aataataccc tgtacagtta
    19861 tgtacccatc ccaagcgccg gatggggggt tatcgtcagc cgccctactg ctgctgcatt
    19921 cgctacctcg caagctactc accggggtgt gctccttacc atcggagtgt tcctgattgt
    19981 tggcttgttt ttctggttag ccctatcccg acaagtgctt cacccattag aacgcctggc
    20041 cgcgttcagc cggactattg gtctggatga ggaattctcc gaggatcatc gcctccagct
    20101 aacaacactg tccgaacgac cagatcagat cgggcacctc acacgcagcc tgacccgcat
    20161 ggaaaaagct atcgaagcgc gcctacaaga gctctcgaca ttacttgaaa ccagcgcggc
    20221 ggtcgtatcc accctcgact ctaatctggt ccttaaccgg attctcgaac aagttgaacg
    20281 cctcatggat atccagatgg gtgccattct ggctctggat gaaaagcgtg gcgtcttccg
    20341 cgctcaagct agccgagggc tctcaaagcg atacacagag aatttagcca tctcccctca
    20401 agaaccgatt tcagtaaccc tgcgagccat ccgtagcggg gaacccatcc aaatcagcga
    20461 cactgaaaag gatagctcct tcaccgcaat gcgcccgcgc gcccgttcag aaggatatcg
    20521 ctctgtctta gctgtgccgt tgaatattaa gcacggacca ccttctgcac tgctggttta
    20581 tcgccctgat ccacatatct acaccaagcg tgaaattaat ttgttgacta gctttgcaaa
    20641 tcatgctgcc atggctatcg agaacgctgc cctctacgca cgcagcgata tgcgattacg
    20701 agaacaaacc cgtcgacttg aggcactgat ccaatcgctg caggatggtt tgatcatgga
    20761 ggaccttcaa gggattgttc ttcacgccaa ccgccggatc ggggagatac ttgaattgcc
    20821 cttggaggag atcatcgggg ttccgggcag ccaactcatc gagcatctgt taaatcactt
    20881 agccagtcaa gataatgcac agcgggcaaa aacaacccgt ttggtggaag atgcactcac
    20941 cggagaaggt tcgcgccgtg ttgaactgga aatcgtgacc aaccataagc tatgctatat
    21001 ggtccttcga acttttgatg tcaccgatgc ggacggcaca ccaattggtc gtggccatat
    21061 cttgcgtgac atcacacgta accgagaaat tgaccgcatg aaatccagcc tgatctccac
    21121 cgtgtcccat gaactgcgta cccccttggc tgctattaaa ggctatgtga ctacactgct
    21181 tgccgaggac gttatgtggg atagcctgac acagcgtgaa ttcctggaga tcatctcaaa
    21241 cgaaagcgat cgtctgagca agctggtcaa cgacttactg gatatgtcgc gcatcgaagc
    21301 aggcagtctg gatgtaaacc gagcgcagtg taatctagaa gaaataatcc agcgcgccgc
    21361 tcagagggcc tatccaaccc caattgaacg gcttgtggtt aacatcccac ccgatctgcc
    21421 gtctctctat gtcgatccac agcgtattga ggctgtctta cgtaacctaa tcgagaatgc
    21481 cgcaaaatat gcgggtgaca tctctccggt aagcataagc gcttcggtgg aaggagaaga
    21541 gatggtagtg cgtgttcgtg atgagggacc cggaataccc gctgagcaca gcaaccggat
    21601 ctttgagagc ttttaccgct tggaaagcaa tctgacacgc atggcacatg gggatgggct
    21661 gggtttggca atttgccagg gattcatcag cgcgcatggt ggcaaagtat ggcttgagaa
    21721 acagaccaaa ggcacatgcg tcgcattctc tctaccactg tctcagatac ccgtggagca
    21781 gctcaaggct gaatgacccg tagttgatta tccagccagg gaacaacctt ctttgtcatg
    21841 gtcaacatta ccgtcctagt cgtagatgat gagaaaccgt tgcgtgattt tgtgcgccgc
    21901 aatctggacg tgcgcggttt caatgtacgt gttgcctcca acgggttgga ggcccttgcc
    21961 atattcaaca cccaaactat cgacttggta atcttagatg tgatgatgcc gcgcatggat
    22021 ggtctagaga ctatacggcg catcaggcag agttcactgg ttcccatcat cgtattgtct
    22081 gccctgggtg aagagagcga taaaatacgc gccttaaacc tgggcgctga cgactacctg
    22141 actaagcctt ttggggttgg tgagttactg gcgcgcgtcc aggccgtcct tcgtcgcgcc
    22201 cgttgggcag aaccaccaac tcgtcaaggt cgactggcgc gaggtgaggt cgtgatcgat
    22261 ctagagcgcc acgaaaccac tgtacagggt aaagagatcg aattgacacc cactgaattc
    22321 aatttgcttg tttacttgat ggagaattcc ggcaaggtat tgccccatca aaccattctg
    22381 cgacacgttt ggggaccgga atacggcccg gagcacgaat acctgcgggt gtatatcggc
    22441 aggttgcgac aaaaaatcga ggacgatcct gccaatccca aatatttgat taccgagcgt
    22501 ggaataggct acagcttcga aggctgatca accagagacc aaacctcagc gctcacaaga
    22561 ggatttccac caccctcccc ggtcgcttat aggctgctta atcaccacgg tgagtctttt
    22621 ggattggtct tttatgtcat atttataaac aatccccccc ttttagccgt tttttgaata
    22681 ttgtatgtac aataatgtag tcaggcaaat acaatttgcc ataacgaact atttcatttt
    22741 tcaaaagaag cagatgaaac ttatgaaaca cgcctttatg accctgctta cgctactcat
    22801 agcggcaaca ttattgctat ctggctgcgc accacaagcc gcaccagaag aaccagctgc
    22861 tcctgaagca cctgcagaac cagctgccac cgagccacct caggaagagc cgatggggga
    22921 agaggtcacc gtagtggttg gctttacctc ttcattgacc ggaagtcagg aagtttcatc
    22981 aaagcgccaa gtaaatggct ttaccttatg gattaatcag atcaatgaat caggtggtat
    23041 tacgttgagc gatggcacgg tagtcaagtt cgacttcaag acctacgacg atgaatccaa
    23101 cgctgaacga gtacaagaac tgtacacccg cctgatcacc gaagataacg ccgatttctt
    23161 gatcagcccg tattcatctg gcctgacatc tgccgcatca attgtagccg aacagaacgg
    23221 caaagtgatg attacagccg gcgcagcaga tgatgctgct tataaaaccg gtaataccag
    23281 tctattccag ttgtacactc ccggctccct atacatggtc agcacggttg atatgcttaa
    23341 agaaatagat ccaacagcta cgatcgccct tgttcacgaa agtgataagt tctctacagg
    23401 cgtagtagat ggtatcaagc cctatctgga atctaatggc tttgagatcg tgctcgaaga
    23461 agggtatgct tcagacacta ccgattttgg tcccatcgtg aacaagatcg ctggttctgg
    23521 cgcaaccgtt ctactcggtg gtggtcacta caacgacggc actgccctgg cccgcgccct
    23581 ttatgatcgc aatattggcc tcaagttcgt ttacctgctg gtagcccccg ccgacaccaa
    23641 attccccgaa ctgggcaatg ctgctttggg tattgctaca tccagccaat gggaacttgc
    23701 tgcgacacat acggaagaag aagccgccaa tttgggggta gaagggttcg gcccctcagg
    23761 tcaagaattc gccactgctt acgaagctgc ttatggcgat cccccgacat atcatgtcgc
    23821 tggtggttat gcagctggtc tggttttgca aaaagccatt attgatgctg actctgttga
    23881 acctgaagct gtcaaagccg ctctgaacgc catgaacctt ttcaccttct acggtggcat
    23941 ccaatttgac acttcagaag aagcccacgg cttgcagatc gctcacaaaa tggtcgttgc
    24001 ccaatggcag aaggatgatg ctggcgaact gaaacgtgtg attattgccc cagcggatgt
    24061 tgccacggct gaacccctct atccaattcc agctccttaa aacggttcac aaaaaactat
    24121 cccctcagcg atcctggggg gatggtttct atttctccac agaaatgcta cattatgcac
    24181 actatcagaa tttagaaaca gataatatgg atcaattcag attcttctat tcctctaaac
    24241 aaaacgtgcg tgcaaccggt gggaatacgc gtccagtttt attacgggac aaacttaaca
    24301 tctatgatgg gagtaattat gcaacaattt agcttgctac tgacatccct cccggatggc
    24361 attttgcttg ggtttgtgta cggtgtggcg gccatgggtc tgacgctgat ctggggagtg
    24421 atgaatgtca tcaacttgtc tcatggtccg atcatcgctt taggaatgtt tggaactttc
    24481 tttattttta tccgactggg tatcaatccc tacattggcc tgctcttgat cgcattcctc
    24541 ggattgataa ttggggtgtt gatttatttc ggagcagtcc actgcgtaat caataccccc
    24601 cacctatcat cgctgttagc gacgttttct gtcaacatga tcattatcgg tctaggaaca
    24661 gccgccctaa caacctcgcc atataatgtt gatttcaata tgggcagttt ccggattggc
    24721 ttaaccacaa ttcttggaac gcgcctgatc gcggcattga tcgcaattct gttcgctggt
    24781 gggctttatc tatttttgta taaaacccga cccgggaagt tcatccgtgc tgttgccaac
    24841 aatcgcgccg cagcagaatt aatgggtata ccctctactc gcatcctggc acttagcttt
    24901 gggctaggca ccatgctggc tgcagtttct ggggcgttga tcgccacatt tttccccttc
    24961 agcatcctat cggggagcgg ttacgaatta aagagcttcg tcatcggtgt tctaggcggc
    25021 ctgggcaatc cgatcggtgc gttattggga ggcttaatcc tgggtgtgct tgagggtatt
    25081 attcccgctt tcatggagac ttcttgggta ccggtcattg aatttgggct gtttgtggtc
    25141 atccttttag ttcgaccgca cggactcttt ggaataaaga aatgaataaa tggcgaaatc
    25201 accttggtct tcttatacca ctattggcag ttgcagcaat ggtcctgtgg ccgatcgtta
    25261 caggaaatgt ttccaaccgc gaaataatgt ttaccatctt gaaggctatc gcattggcct
    25321 ccagcctgaa tattttactc ggttatactg gctatgttag ttttggccat atcgtattct
    25381 atgggtttgg cggctatgtt ggtttgtact tagtcaacga acaaggctgg tcgatatggc
    25441 tggggatgct ggttggcgga ttagcctctg gggctttggc ttatttgctg ggtaaagcga
    25501 tcttgcgttt acgcggcgct tactttgctt tggcaacgat cggcattaat gaagccatga
    25561 aagcattcat caataatttt gagcccttcg gaggccctat cggcatgaca ctcaacttct
    25621 cagtctacaa agcctatgga ggcgcagccc aggcattgag catgtcattt tatattatgg
    25681 ccttcctgac atttgccacc ctcatcatta gctacctggt ccgcacctcg aagtttggct
    25741 taggtctgcg agcaattcgt gagaacgaag acgctgccga agtcatgggt gttgtagctc
    25801 ccgaagccaa gacctgggct tatgtgcttt cagccatcat acccggaatg gtgggtgtgt
    25861 tattcttctt caaaaatggt aacgtggagc catatgtcgc ttttcccctg cacgcctcca
    25921 tcgagttatt ggttatggtg atgttgggag gtcagggtac cgttctcgga ccagtccttg
    25981 gggcactggc ctaccaaggc atgcgcggct atctcgtaac cagtactttc ttcaaagata
    26041 ttcagctttc cgtttccggg gtgcttttgt tattgatcgt gctctttatt ccggcgggtg
    26101 ctatcggctg gctgcgccat cgcttctcag ttctccacaa ggtgctacca tgatcttaca
    26161 agtagaagga gtatccaaac gcttcggcgg gcttcaagca ctcatcaggg ttacattcga
    26221 cctaccagat gggcagattt tagggctgat cggacccaat ggcgcaggaa aaactaccct
    26281 gtttaatgtc atcaatggag tctatccacc caatgaaggc agggtaactt ttcggggcac
    26341 tgatgtcacc gggatgaagc cttatcagct agcccacctg ggcttagccc gctctcacca
    26401 gattgtgctt ccactcaacg agctcagtgt gcgcgagaac gtcatggtgg gcgcctgctt
    26461 tgggagagaa aaacaaagcc ttggcggagc aggcaaaaca gcagaagaaa tcctggattt
    26521 cgtcggcctg gcggatcgtg cagaacaact tgccggcagc ctgaacatcg ctcagaagaa
    26581 acgcctcgag atggcacgcg ccctggcatc ccggccccac ctgctgctct tggatgaagt
    26641 gctggctgga ttaaaccctg ctgaaatcac aacaatggtc gagactatcc ggcgcatacg
    26701 ccagcaaggt gtcacgatca tcatgatcga acatgtcatg cacgctgtaa tgaacgtttc
    26761 tgaccgggtc atcgtgctgg attacggtga gcagatcgcc gaaggcaccc ccgaggaagt
    26821 atctcgtaac gagaaggtaa ttgaagccta cctgggcgat ccaaagcttg ctgagagctt
    26881 attaaaacga aactagtagg gatgaagtaa tggctatctt tgaaattcgc gatctaactt
    26941 ctggatacgg cgaggtgcaa atcctatggg gtgcaactct ctcattggat gaaagcaagc
    27001 tcacttcgct ggtaggaggc aacggggcag gaaaaaccac tatgctgcgc acgatcatgg
    27061 ggttgacccg gccatgggaa gggtcggtct ggtttgaagg caaagatatt tctaccctgc
    27121 ctccatatat gaaagcagag atgggcctag tattggtacc cgaaggccgc cagctcttca
    27181 ccgacatgtc tgtagcagag aatctcgaaa tgggagcttc gcccaaacgc gcccgccctc
    27241 actacaaacg taatctagag cgagtctacg atctcttgcc acgcttgaaa gagcgtcagc
    27301 atcaaaaagc tggcacactg agtggtggcg aacagcaaat gctggctgtt ggcaggggta
    27361 ttatggcgga accaaagata ttgatgatcg atgaactctc acttggcttg gcgcctgtcc
    27421 tggtactcag cctgtttcaa acccttctac aactcaaaga tttaggtatc acgctcctgc
    27481 tcgtagaaca gaacgtacaa atggccctgg cagtcagcaa ttatggttat gtgctctccc
    27541 agggacgcgt cgatctgcag ggtccaagcg aagaactggc acaaaatgag cacataagag
    27601 cagcctatct aggtttataa ttcatactaa ggaggctact atgttagttg gcgaacgcat
    27661 gaatcaccct gttataacca tcaacccgga tatgccggta gtcgacgcgc tgaaactgat
    27721 gaagcgcgag cgtatccgtc gtacaccagt gatcaaagat ggcaaactgg taggtatagt
    27781 atcggacaaa gatttactaa acgcctcacc ttcaccggca acctccctca gtgtatggga
    27841 acttaattat ctgctgagca agatcaccgt cagcgaggtg atgaccagaa aggtgttgat
    27901 cgtcagcgaa gacaccccca tcgaagaggc cgcgcgcatc atggcggaca ataaaattgg
    27961 tggtcttcca gtgatgcgag gcaacaactt ggtcggcata atcactgaaa cagacctatt
    28021 taagatcttc ctggaattaa tgggggctcg atatatgggt gtgcgcgcca ccgtgctggt
    28081 tcacgaagag cgcggccagc tcgccaagct gagccagaca atcgccaatg caggtggcaa
    28141 ttttgtcgcc tttggcatgt tccaaggcga agtcccaacg aaccggttgg tgaccttcaa
    28201 ggtagatggc atcgataagg agcaacttaa ggcagtactt gagcctattg tggagagtgt
    28261 ggtagatatt cgcgataact caaaataata ccgagctttt taccaagata ttacattaaa
    28321 cgattaacca gaaagtactt ctttcctggg ataattagga ttgacagaaa aacggggcat
    28381 cgggtggtga agtcattgcc cgacaacccg tttttcgcgt aatttcgtct gcccttcggc
    28441 ctgtattaat ttccaagcgc gctccacttg atcgacttcg ttggcggtct ccggtagctc
    28501 tccataacgg acatggagat aagccttcgt gatggcttct aactccatca tcaagtcagg
    28561 aaataagccc cgcagactgg gtaaaaactc caatggcgtc tgggaagacc ggcggggaga
    28621 atccaggcgc gcactcaatt ttagtaattg ggcgtatatt cggcgtacac gcgccgcggc
    28681 cagccttcgc tggatttgcc ttaacccaag catgctatct acctgattaa ttaattgatc
    28741 tactgccctt ctcaaacctt ttcgtagcag atcaaataaa tcgggttgat ctatgtcagc
    28801 tagatcttct tcctcgttgg caatattttt ccatctctgc ttctgaaggc ttatcaaaat
    28861 cagaatgaca aagaacaaga tcgtccccca aagaactgcc gactttggaa ttttgagcgg
    28921 ttgaagccaa ctcaaattaa tgaggcgatc caggttacct atccatgagc gcacaaattc
    28981 catcaattcc gatataaata accccagcct atcaaataat gcgatcagta tttgcagtac
    29041 tccttcaaaa cttatgaact cggtgagcag acgaagcagc tcagcgatcg cccagagcaa
    29101 ggggctaatc agaaaaacaa ggatcgagat aatccacgag tacacagtga agatcgtttc
    29161 gagacccccg ctgcgcagcc aacttactgc ccaatatgcc agcccaacca ccaaagaggt
    29221 ggtcagcaat atagcagcca tccatttccg atcaaaagga atggtcttcc ctccacgcat
    29281 gtgagcttga gaggcgatac gcgccgcact gatgcccacc aagctggaaa aaatgaacag
    29341 gtaaaatgag aagccgggag atgtttctct cagatgaaca accaacccaa agataaacag
    29401 catgatgatg cctgtacgga atcgaccaat gatcgactcg ggttcaagca ttgccccaac
    29461 aagcgataaa cctcgccaac tcatcatcaa gctggctaca atgacaagaa actccccagg
    29521 gatcgagacg gctacatcgt tgaaagactg aactggacta agcacaaact ctttcaggct
    29581 ttgccactca cccggattca gcagtgttac taacccaaaa accacactta tcagcatcac
    29641 gccgagaata ataacccgac gcttagcagc atgaatattt ctgcggagat tcaactcatc
    29701 caaatagtat gaaaacagga gaatagcccc acaggttaag aatgccctcc agtagctgat
    29761 cattatcctg gtcgcaagaa gcaattgaat ccacaatgcc acccagctca gctccatggt
    29821 aacagcggca aaaaacgaca atgttcgcca ggcttgaggc tttattctcg gctcgctcgt
    29881 tggctggttg gctggagaga ttgtcccgtt cacaagctca catccatcgt gatcacatct
    29941 tggttaatcg tgcaaggaca catctacacc tcttcctcct tgaaaggcat gtgcaggcag
    30001 cgcacgccca agatatcggg cggtggttct tcagctaacg agaacaatgt gatttgccgt
    30061 tcgtgtttct ttaatctaga gagagtctct accagggcag gggatgtcaa agcagtcacc
    30121 acaaccagtg tcgagccgta aggaacatgg ggtatttctc gcatcaagaa gttctcgaaa
    30181 ggtttcgcca tcactggcga tgcacccgcc agcgcctcta acaagcgtcc caattgtttg
    30241 ggggagcgcc caggggggat atgaaacggt tggtctgaat tcgaaaggca tccattggat
    30301 atcagaccaa cccgataacc atgctcaatg ccttgatata acaaagtcgc cgcgatactt
    30361 agcaagcgct caaataatgc aggatttatt ccttcccagg aatgctgata cgtcaagacg
    30421 ttcaggcata cgaccagggt cagggctgac gttggctgga aaactttcac ttgcaattgt
    30481 cctgtgcggg ctgtcgctgg ccaatgcacg cggcggaagc catcctccgg gcgatattca
    30541 cgtaccccca tcgggaagtt gggatcttcg agaaatcgcc ttcgagagcg ttgatcgcca
    30601 aaggggtttt cagggggcag cttcaactct tcgaggggaa taagcgttgg aaagaccgtt
    30661 atctgatcta ccgaacccaa acgatcagat tgtttataga ttccaaaaaa atcaccagac
    30721 tgcacctgta ctggtcccac tttataaact ccacggcttc tcagatatag ctcgtacatg
    30781 cgccgagccc tctcaaacca acgtaaactg aatgcgtgga tcagtaatcc ctggtctgcg
    30841 atgtggcttg gcgtgagcgc gcgctcgtcc tccggaccca cagcttttgg ccagggatcc
    30901 gtgacgcgca accaggataa tggaagcaat ttattatttt cgatctcaag ccgtacggga
    30961 aagtgctcac ctgggaagcc gcgtgtataa cgaaagcgcc gcgtatagcg caccccatcc
    31021 agggcgtgtt tatgccacca gtaggctatt ccaaaaatga caagcagtgt ggaggagaag
    31081 gctgtcagaa atggcgagcg cagaactatc ccaagataaa agatgataat gaataagggg
    31141 agccagcgct ggatgggcac gtcagccctc cacgggcacg ggcacagcct cgacgatgtc
    31201 cgagattaac tcctcgatct tcctgccacg caactgcgcc tggggagaga tcatcaggcg
    31261 atgactgagt atgtatggcg ccaaattttt cacatcgtca ggcaaaacgt attcgcgtcc
    31321 gtgaatggca gcccaagcct gggctgcttg gtagagtgaa agggttgctc gggggctggc
    31381 acccaactca atctctgtat tttggcgggt tgcgcgagcc actcgcacaa tatactctcg
    31441 aacaggctca gcgatacgca cttgccggcg ctggttaatg tactcgagta tctcctgcgg
    31501 tgtagtcact ggctccagat ccaccaaagg atccgctagg cgaaatcgtt ccaggattgc
    31561 attctcttct gattcttcag ggtagcccaa agcaatccgc atcaagaagc ggtcaatttg
    31621 cgcttccggc agtgggaaag tcccttccag ttcgatcggg ttctgcgtgg ccatcaccaa
    31681 aaaaggtcgt ggcagcgggc gcgttacccc atccacagtg acctgccgtt cttgcatggc
    31741 ctccaataat gcagactgcg tgcgcggcgt ggcgcggttg atctcatctg ccagcaccac
    31801 ctggcttatc accggacccg aacgaaactc gaattcttgc gttttctgat taaaccagct
    31861 gatcccggta acatcagaag gcaacaggtc aggcgtaaat tggatccggc gaaatgtaca
    31921 gcccaacgaa gctgctaagg ctcgagccag ggtggtttta cctatgccgg gaacatcctc
    31981 aagcagcatg tggccctcgc agagcaccac tgtcagggtg aggttactca cctcatcctt
    32041 accgatgatt accttttgga tattttgtcg taagcgttga gcgatatcaa taatcatttt
    32101 ctacaatgaa gtttaccaga aataccatca ccagccatta caacggctgc ctcggatatt
    32161 cccaaagaac taatatcatt acccgcgaca caggtatcaa gttcatcaaa tgattagcgt
    32221 ctttagatat ccgtggtcag agtggtatac ggcatttcta cagcagagta taatcctttg
    32281 catgagctct atcgttgtcc tggatatcga aactaccgga ctggattcgc aaaaagacgc
    32341 tgtcatcgag atcggtgctg tgcgcttcaa cggtcgtcgt gtcgaggacg aatggagcac
    32401 gctgatcaat ccaggtcgcc gcatcccccc ctatatcacc cagctgaccg gcattacaga
    32461 tcaaatggtt ttgcaggctc ctgccattaa gaatgtgctg caagagcttg ttgatttcac
    32521 tggaaatagc cctgtcctcg gtcacaatgt tggctttgat ttatccttcc tacgccggta
    32581 taacattctg ctgacgaatg agcccatcga cacctatgaa ctagcctctg tattacttcc
    32641 tggtgcgcgc cgttacaacc tgggcgccct ggcacaggag ctgagcgtcc cgctgcgggc
    32701 cacccatcgc gccctggacg atgcccatac cacgcgcggg gtcttcctgc gcctcattga
    32761 tctgatcatg gagttaccac tgaatttatt ggcggaaatc gtgcgcttga gcgagccgct
    32821 cgagtggggt ggctcgtggg catttcggca agctctgcgc tcacgcacga aagaaatcgt
    32881 caatgcccaa caggttcgtc acgcctacca gggtccaata tacactgaag ccttaccccg
    32941 tccttcggct cctctacatc ccttggaaaa acctgtttcc ctggatatcg atgatacggc
    33001 tgcgttacta gagcatggag gggcattctc acatcatttc ccgcagtatg aataccgacc
    33061 ccagcaagta gccatgttgc gagctgtcgc agaggccata tcctatagca ggcatctgat
    33121 ggttgaagct ggcaccggga cgggaaaaag catggcatat ctcatacctg ccacgctgtg
    33181 ggctatccag aacaacaacc gcgtcatcat ttctaccaat accatta
//
LOCUS       DBNGSX010000082        18310 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_490746, whole genome shotgun sequence.
ACCESSION   DBNGSX010000082 DBNGSX010000000
VERSION     DBNGSX010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 18310)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18310)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18310
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_490746"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="AC16CB_02655"
     CDS             <1..230
                     /locus_tag="AC16CB_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341397.1"
                     /translation="ERLRLIRICLERLRFLASAAPLLVSASPDSGPPMSGGRGRRQLQ
                     PDDRLLALLEEAADQVWRFGPPPPTVQLRLL"
     gene            complement(476..1756)
                     /locus_tag="AC16CB_02660"
     CDS             complement(476..1756)
                     /locus_tag="AC16CB_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_246585292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HIR3341398.1"
                     /translation="MSKKELSRLEIMQRIQEKRLTQKEAAWMLGLSVRQVKRLYRAYR
                     QQGAAGLVSRRRGKPSNNRMDPQVEQQVIDLIHERYRDFGPTLAHEKLVELHGLKISD
                     ESVRKLMIAERLWKPKKAKRSAVHPLRERRACLGELVQIDGSEHAWFEARGPKCVLLV
                     FIDDATGQLGELRFVPQETFFAYCEASRHYFEQHGKPLAFYSDKDSIFRVNQPRPLGL
                     SSGQTQFARAMEELDIQTLCANTPQAKGRIERVIQTLQDRLVKELRLRGISDMQAGNA
                     FLPEFRADFNRRFGVVPRSSHDAHRPLLPSDNLERILTHQEIRTLTKNLTVHYHNVVY
                     QIHTQRPTYALHKAPVTVCENARGEVTLLYKDQPLDYSTYHKPQRQAEVVSSKTLDHQ
                     LRLPKPPPANHPWRRYGRHLNGKPIQKVFPDGAD"
     gene            2035..2352
                     /gene="rplU"
                     /locus_tag="AC16CB_02665"
     CDS             2035..2352
                     /gene="rplU"
                     /locus_tag="AC16CB_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR3341399.1"
                     /translation="MKYAIVEDGGKQYKAVEGATIDVDRFVADEGDQLDMERVLLVVD
                     GDDVKVGTPLVAGAKVQATVISQVKGPKVVVFKYRPKQRYRVKKGHRQQYTRIMIDSI
                     SVE"
     gene            2363..2626
                     /gene="rpmA"
                     /locus_tag="AC16CB_02670"
     CDS             2363..2626
                     /gene="rpmA"
                     /locus_tag="AC16CB_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR3341400.1"
                     /translation="MAHKKGGGSSRNGRDSQSQRLGVKVFGGQFVSSGSILVRQRGTR
                     IKPGRNVDVGRDDTLFATSDGIVVYETIRDDRKRVSVIPEPTA"
     gene            2677..3150
                     /gene="rpmE"
                     /locus_tag="AC16CB_02675"
     CDS             2677..3150
                     /gene="rpmE"
                     /locus_tag="AC16CB_02675"
                     /inference="COORDINATES: protein motif:HMM:NF015102.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIR3341401.1"
                     /translation="MKSEIHPVFYPDAQVICACGNTWTTGSTKKVIHTDVCSNCHPFY
                     TGEQRIVDTEGQVDRFYKKLQARQQYLSEVEAREAARISPERPITELDLGPRVTDKLA
                     KAGIVNVGQALDKLALGEASLLSIDGFGRKSLADLKKRLRSFGYSIPEEGEEITV"
     gene            complement(3258..3521)
                     /locus_tag="AC16CB_02680"
     CDS             complement(3258..3521)
                     /locus_tag="AC16CB_02680"
                     /inference="COORDINATES: protein motif:HMM:NF051948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341402.1"
                     /translation="MQDLQAQTEKIRSVRRAYEKALMSKANVVGVGLGYRHHAGKRTD
                     ELALVVMVNKKVPLTQLAPEDVIPTKLEGVTVDVQEVGELKAF"
     gene            complement(3529..4536)
                     /locus_tag="AC16CB_02685"
     CDS             complement(3529..4536)
                     /locus_tag="AC16CB_02685"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chymotrypsin family serine protease"
                     /protein_id="HIR3341403.1"
                     /translation="MAERAAIQRVKDAYKEEILSKPHVVGVGTGYKISKGVRTSEMCI
                     VTLVRQKMPVDGLSPKAIVSKQYDGILTDVIEVGDVRALQTPTSRWRPAPGGVSIGHY
                     KITAGTLGAVVRDRASGDRLILSNNHVLANSNDAAVGDPILQPGPTDGGSTSSDVIAH
                     LERFHPIDFSQEPGTCSLANSFAELGNAIASAMGAKHRVQAIYANPQAANLVDGAVAR
                     PVSDNDIKDEILNIGVVSGTMEATLGMSVRKSGRTTGFTTGEITVLDATVNVSYGTGR
                     VARFDNQIVTGPMSQGGDSGSLVVAGDALKAIGLLFAGSDQTTIINPIQAVLDSLSIT
                     I"
     gene            complement(4809..6011)
                     /locus_tag="AC16CB_02690"
     CDS             complement(4809..6011)
                     /locus_tag="AC16CB_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3341404.1"
                     /translation="MRSNSISKRINNVMTSSQPFIKFFTNPVFEHRNDDPLACDFALG
                     NPQEMPLAEYVNALQRWITPGNKDWFAYKTSEPAAIAALDESLYRWRGIRYSPEDIFL
                     TSGAFASLNVTLGAILDPGDEVIFISPPWFFYEPMILSHNGVPIRVRINSVTFDLDID
                     AIAAAITAKTRAIIINSPNNPTGKIYPPQTLRQLADALHAAQKKIGRPIYLLSDESYS
                     RIIYDGIQFPSPTQYYPYSFLIYTYGKTLLTPGQRIGYIVLPPKMPDRGQMREALFIS
                     QVVNGYAFPNALLQHALADLNELSIDVDHLQQKRDYLVGELRNMGYELHFPDGTFYLL
                     PRSPWKDDEAFVEMLQSNKIFCLPGTVVEMPGYFRISLTASDDMIQRALLGFAAALEK
                     AGQIAQSM"
     gene            complement(6122..6907)
                     /locus_tag="AC16CB_02695"
     CDS             complement(6122..6907)
                     /locus_tag="AC16CB_02695"
                     /inference="COORDINATES: protein motif:HMM:NF015534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HIR3341405.1"
                     /translation="MKSMIALIGGEEFADGFEDVHANLLELSFSNRSQKDGKPVQVVF
                     LPTCATDDGPDTVTYWCDEATKRLEAVGAKVKPLRIVDRPSADDPENARLIIEADWIY
                     IGGGYPHVGMRILRGTRAQQALEAAWQRGALIAGASAGAMMLGAQSWVITPELDAAVT
                     KLFSQGAGISEWDLPLPPALECLGFVPRSMCWPHANQLFSLRWLKSGLLPPHHFVIGI
                     DEQTAFVQTTNRGWQVIGRGKVSLVDDQFQVTELNSGSRIENL"
     gene            complement(6914..7723)
                     /locus_tag="AC16CB_02700"
     CDS             complement(6914..7723)
                     /locus_tag="AC16CB_02700"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HIR3341406.1"
                     /translation="MNETLKLMCIMAHPDDETLGVGSTLAKYADEGIEVYLVTATRGE
                     RGWFGTREDFPGKEALGRIREAELRCAAQTLGIKQVEFLDYIDGDLDKADPTEAVGKI
                     VESIRRVRPDVVITFPPDGAYGHPDHIAICQFTTAALVSAADPTYPVKNQLQPHRVSK
                     LYYTADNQELFEIYTQIFGDLRMTIDGKERQAVAWSDWAITTHIDGEKYWPSTWQAVQ
                     CHASQIAGLGDRERITEEQHRRLWSERTYYRAYSLVNGGRKIETDLFAGLR"
     gene            8122..8694
                     /locus_tag="AC16CB_02705"
     CDS             8122..8694
                     /locus_tag="AC16CB_02705"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019153983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HIR3341407.1"
                     /translation="MKHHTGSIEVITGSMFCGKTDELIRRLRRATIARQRVQVFKPVI
                     DDRYSVEKVTSHAGSEYAAQPLQHAAEIPSLIEPDTTVVGIDEAQFFDSDIVPIVQSL
                     ADRGIRVLIAGLDTDFRGEPFGPMPLLMAAAENVDKLHAICMMCGEAASRTQRLVNGK
                     PAHYNDPVVIVGASELYEARCREHHDVPRN"
     gene            8716..9273
                     /gene="hpt"
                     /locus_tag="AC16CB_02710"
     CDS             8716..9273
                     /gene="hpt"
                     /locus_tag="AC16CB_02710"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIR3341408.1"
                     /translation="MQGYKEFLDEILITEEALQNRIAELGAQISKDYEGKQPHLICIL
                     RGGVMVLSDLIRHLTVPNTIDFMAISSYGVGARQSTGKVRITLDLQEDIIERDVLLVE
                     DIVDSGYTIATVLEFLKTRHPSSLRVCTLLNKAERREIQVPIDYCGFVIPDKFVFGYG
                     LDLDELYRNLPFIGTVNPERYHPPV"
     gene            9324..10940
                     /locus_tag="AC16CB_02715"
     CDS             9324..10940
                     /locus_tag="AC16CB_02715"
                     /inference="COORDINATES: protein motif:HMM:NF013871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA nucleotidyltransferase/poly(A)
                     polymerase-related protein"
                     /protein_id="HIR3341409.1"
                     /translation="MGRWVARLGERIIGQGGTPEQALWAARAARSKENPQIDYIPTVQ
                     PLVFSARLEPIRSLMPVDMPAYLVGGAVRDALLMRTTHDLDFVLPSGALKAGRAVANA
                     LRGAYYPLDAVRQTARVILYAPDGTREFLDFAVIRGEDLESDLRARDFTINAMAIDLR
                     HPTQLLDPLGGAADLLARQLRACSPRAFLDDPVRILRGVRLAAELNLKIQPETLKIMR
                     QSVGELERVSSERKRDELFRILDGPQPAKALRALEILGALVYVLPELVSLKNVMLAPP
                     VDTNVWTHTLDVVRNLATLLDVLDLKYKPDTAANLSMGLVSMRLGRYREQLAEHLKSS
                     ITPERPLRSLLFLAALFHEVAQSQIPQVDESAGDMIDNDIAGAQMVEERGEALHLSND
                     EIARLKIIVRFHRRTILLAQEEDPPSRRAIYRFFRDVGDVGVEISLLSLADTLATYGP
                     APHQDMWARHINTVRMLYEAWWEHHQEYISPPALLGGHDLIDELRLKPGLHIGQILEA
                     IKEAQATGQVTDRDGALSLARSWLAEHSID"
     gene            complement(11344..11934)
                     /locus_tag="AC16CB_02720"
     CDS             complement(11344..11934)
                     /locus_tag="AC16CB_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3341410.1"
                     /translation="MNNAALTRVLVIDDDAAMTEMLRLILEPNAFDVKVSHSGREAIE
                     SAKHLSPEVIILDLSLEDMDGGEVCKQIRSFSQAPILVLSAVSRPGMVAKALDEGADD
                     FLLKPMTSSVLIAHLKRLVWRARAEENANHRTDKSSQKAQPSTGSTLHRNSEPRRGKD
                     IRALHAAKYPSQPKDDSTLARNKAQRRISTDKPKKV"
     gene            12124..13281
                     /locus_tag="AC16CB_02725"
     CDS             12124..13281
                     /locus_tag="AC16CB_02725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3341411.1"
                     /translation="MSSQIHCPQCGQPTESSSRTCEKCGVVLAIAAVLAEREILPHRI
                     DGSLAVTPEILVPRLGEYLVEKGKLTRPNLERALDYQKKKSRSGKTLLLGQALRELEL
                     ITAEELDQAITEQILNLQVALRRSNQELEARVRERTIELERALEMLGELNQLKANFIA
                     NVSHELRTPLTHIKGYLGVMATGELGPISVDQADALRVMQQAEARLERLIEDLIQFAL
                     ISRGQLTLSLSHANIAEMVENVVNQVQQKANLAGINLETDISEGLPLAVCDNDKIAWV
                     IGHLLDNACKFTPRGGRVKLEVDQSDKFVKIAVTDTGIGIPAHRMPEIFEPFHQLDGS
                     STRQFSGTGLGLALSQRILTAHGTQIEVQSQEEKGSRFEFLLPLAEVAMQM"
     gene            13290..14540
                     /locus_tag="AC16CB_02730"
     CDS             13290..14540
                     /locus_tag="AC16CB_02730"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3341412.1"
                     /translation="MVDETSNPTALRPEELEAVYSISRAVAGAVDLDTALDEIIRLTR
                     SVFIFDNMVLYNLRSIDTLEPAYARAIGRGRSQEAELVWGEATALEAIQKGQVIILQE
                     ESGDQEDRTGVRFSLGLPLRSGKDIKGALVFIRFGGPTYKPDQIRLAEFIAMHVAQLL
                     EHGQLVQRVVSLEAERRLDRLQEDFIATVSHELLTPLGFIKGYATTLLREDAVWDDAT
                     RREFLSIIDEEADRLRELIDNLMDSSRLQAGALRMSFQPVRMDTFLKDLSFRAKSRDE
                     KQKINLELYSPDLIIQVDPARLAQVFDNLLSNANKYAPGAAISITLDRVNELAHIRVK
                     DDGPGIDPEHLPNIFKRFYRVPSNNTTVRGTGLGLFICRQIIRSHGGNISVESKPGDG
                     TAFDIYLPIDRVQTEEQISLRESN"
     gene            14540..15235
                     /locus_tag="AC16CB_02735"
     CDS             14540..15235
                     /locus_tag="AC16CB_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3341413.1"
                     /translation="MSTKILVVDDEPRYLRLIEANLASDGFTVIKAMNGQEAVEKVAS
                     HQPDLVLLDIMMPVLDGFGATERIREFSNVPIIVVTARGEEGARVRGLDLGADDYIVK
                     PFSATELLARVRAVLRRAQVSGGSFQQSIFNHGNLKIDFARAEVFRNDRVVFLSATEY
                     RLLLQFAHNLGQVLTSEQLLTNVWGQEYRDDKEILWVSISRLRQKLEDDPKNPIHIVT
                     RSGLGYTMPPMQD"
     gene            15259..15936
                     /locus_tag="AC16CB_02740"
     CDS             15259..15936
                     /locus_tag="AC16CB_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3341414.1"
                     /translation="MVNPLSISLRSKKLGVLIRDARLAARKSIEECCAVIEVSPEEFE
                     AYELGDKSLSLPKLEVLALYLGIPLDHFWGSKARSEDASPALDFDVKQLLRVRQRMIG
                     ALVRQARTQGDISLEELSQEVGIAPDQLEACENGEAELPLPTLEAFCARLNRPIEEFF
                     DKQGPVGVWIGQQQAVQDFLQLPDEIQTFVAKPINRPYLELAQRLSEMSVDKLRALGE
                     GILEITL"
     gene            15945..16496
                     /locus_tag="AC16CB_02745"
     CDS             15945..16496
                     /locus_tag="AC16CB_02745"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3341415.1"
                     /translation="MNEQISVNQLISQGKSAYQQGDFIAAARAFDAAGDSYSVSGDEL
                     NAAEMRNNSSVAYLQAGEAQAALVAVEGTPAIFATAGDLRRQGMALGNLAAALEAMNR
                     LEEATKAYLQSAELLESIGEKDLRAYVMKSLSALQLRSGRHIEALSTMQAGLDGIEHP
                     KPQQRLLKRLLNVPFKFLGKSSD"
     gene            16574..17434
                     /locus_tag="AC16CB_02750"
     CDS             16574..17434
                     /locus_tag="AC16CB_02750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3341416.1"
                     /translation="MIANLLQLTVRPVSDKDLQRLANLIHFEALVHRHLDWRAPLDWV
                     GYNPYLVAEHNDEILAALACPPDPPSVAWIRLFATSSEISVNLAWEELWNAACDQICN
                     LGDTALVAAIPLQNWFRKLLEKSEFVRSHNVIVLSWRRAPRPPASRALPGFIRTMELR
                     DINAVEELDKAAFGLIWQNSYSSLEVAFRQAAVATVLDNDGEIIGYQISTATPVGGHL
                     ARLGVLPGYQGQGIGYAILDDLLVQFERRGAHNVTVNTQSDNLNSLALYKKAGFKRTG
                     EEYPVYQYSP"
     gene            17551..18294
                     /locus_tag="AC16CB_02755"
     CDS             17551..18294
                     /locus_tag="AC16CB_02755"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR3341417.1"
                     /translation="MAKKQRPGTPSKRQIIREQRMKRQQQQRLLTILVIVVAAVVIAG
                     LLIWPSLRPVGEIKTITPVERPQEDGTALGDPNAPVLVEVWEDFQCPACTGYSQQIEP
                     QVVDNYVATGKVRYVFRHYPFIDDRVATKESDQAANASMCAAEQGRFWDYHDIVFANW
                     NGENAGAYSDKRLIAFAESIGLKMDDFNACFDANRYKDQIDKDFADGTDALVQGTPSV
                     FVNGVIVKPGFVPSYPEIAAAIDAALASQ"
BASE COUNT         4389 a         4472 c         4865 g         4584 t
ORIGIN      
        1 gggagcggct tcgcctgatc agaatttgtc tggagcgttt gcgcttcctg gcttcagctg
       61 caccgttgct ggtgagcgcc tcgcccgata gcggtccgcc gatgagcggg gggcgcggtc
      121 gacgccagct tcagcctgat gatcgactgc ttgccttgct ggaggaagcc gccgatcaag
      181 tgtggcgctt tggccccccg ccgccaactg tgcagttgag attactctag caattatccc
      241 tgtcaagcca aagtagaaat gtcccttatt tgacaaagta gagatgtccc ctttgaagag
      301 ggcagataga aaggcatggt agactccaag cactcgtgcg gagggtacgg cactgctgga
      361 gagcaaccgg agcgctggca cgagtgccga agactgggca gagcggtgct cgctctgcac
      421 tttgcattct gcccagtttt tggccgaaag cgataaactc aagagccagc tcaaattaat
      481 ctgcgccatc tggaaagacc ttctggatgg gtttgccatt gaggtggcgg ccatagcgac
      541 gccaggggtg attggcaggg ggtggcttag gtaggcgcaa ctggtggtcg agggtcttgc
      601 tggagaccac ttctgcctgg cgttggggtt tgtgataggt ggagtaatcg aggggttggt
      661 ctttgtagag cagggtgact tctccacggg cattttcgca caccgtgacc ggggctttgt
      721 gcagggcata ggttgggcgc tgggtgtgga tctgatagac gacattgtgg tagtgcacag
      781 tcaagttctt ggtcagggta cgtatttcct ggtgggtgag gatgcgctcc aggttatcgg
      841 agggcagcaa aggtcggtgg gcatcgtggg aactgcgtgg gaccacccca aagcggcgat
      901 tgaaatctgc ccggaattca ggtagaaagg cattacctgc ttgcatatct gagatgccgc
      961 gcagacgcag ctccttgacc aggcgatcct gcagggtctg gatgacccgt tcgatgcgac
     1021 ctttggcttg cggggtgttg gcgcacaagg tttggatgtc cagctcttcc atcgcccgtg
     1081 caaactgggt ttgcccactg ctcagtccca aggggcgggg ctggttgacc cggaagatgc
     1141 tgtctttgtc gctgtagaag gccaacggct tgccatgctg ctcaaagtaa tggcgtgaag
     1201 cttcacaata ggcaaagaaa gtctcctgtg gtacaaacct caactctccc aactgcccgg
     1261 tggcgtcgtc gatgaacact aacaggacac atttcggccc acgcgcttcg aaccaggcgt
     1321 gctccgagcc atcgatctgc accaactccc ccaaacaggc tcgccgctca cgcagcgggt
     1381 gcacggctga ccgcttggct ttctttggct tccacagcct ttcagcgatc atcaacttac
     1441 gcacactctc gtcagatatc ttcaacccat gcaactccac cagtttctcg tgcgccagcg
     1501 tcggcccaaa gtcccgatag cgttcatgga tcaaatcgat gacctgctgc tctacttggg
     1561 ggtccatacg gttgttactc ggtttgccac gccgtctgct caccagcccc gctgctcctt
     1621 gttggcgata agctcgatac agtcgcttca cctgtcgcac gcttagccca agcatccaag
     1681 ctgcttcctt ctgcgtcaaa cgtttctcct ggattcgttg catgatttct aatcggctca
     1741 gttctttttt gctcattgtc agtaggttgt ccactttgtt gtccctttcg aaggggacat
     1801 tttaactttg gtaaaagggg actctactac tttgggctaa cattatttct cgcattggtt
     1861 gacaaccagt ctcctctcag gtataatccc tattcgcact gccccggctg caagctgctg
     1921 gggctatatc ttgtaccccc aactttcccg tacctgggcg catccttctt ggtggatgct
     1981 ggaagcgatc ggagcgcgtg gactacggca aagtgaagct tggaggaatt tcaaatgaaa
     2041 tatgccattg ttgaagatgg cggtaagcag tataaagccg tcgaaggggc cacaatcgat
     2101 gtcgaccgct ttgtggctga tgaaggggac cagctagaca tggaacgcgt cttgctggtg
     2161 gtcgatggcg atgatgtgaa agttggcact cctctcgttg caggtgccaa agtacaggcc
     2221 acagttatct ctcaggtgaa aggtccgaag gttgtggttt ttaagtacag gcccaagcaa
     2281 cgttatcgcg tcaagaaggg gcatcgccag caatataccc ggattatgat cgactccatc
     2341 tcggtggaat agcgaggtaa ctatggctca caagaaaggt ggcggctcca gccgcaacgg
     2401 aagagatagt cagtctcaac gcctgggcgt taaggtcttt ggcgggcaat ttgtaagttc
     2461 tggaagcatc ctggtgcgcc agcgcggcac gcggattaaa ccaggtcgca atgtagacgt
     2521 cggtagagac gacactctgt tcgctacgag cgatggcatt gtggtctacg aaactatacg
     2581 cgatgaccgc aagcgcgtta gcgtgattcc cgagcctact gcttgatgtc ttccacatat
     2641 gtggaaagaa ggctattaaa gttaaagggt taacttatga aatccgaaat ccatcccgtt
     2701 ttttacccgg atgcccaggt gatctgcgcg tgcggaaata cttggacaac cggctcgact
     2761 aagaaagtca tccatactga tgtttgctca aattgtcatc cgttctacac tggtgagcag
     2821 cgcatcgtgg ataccgaagg tcaggtcgat cgcttctata agaagctaca ggcccgacag
     2881 caatatttaa gcgaggtcga agctcgcgaa gcagctcgca tatccccaga gcggcctatt
     2941 acagagcttg acctaggacc gcgtgtcacc gacaaactgg ccaaggcagg tattgtcaac
     3001 gttggccagg cgctggataa gttggcttta ggggaggctt ccttgctcag catcgatggt
     3061 ttcggtcgca aatcgctggc agacctaaag aaacgattac ggtcgtttgg ttactccatc
     3121 cctgaggaag gcgaggaaat cactgtttaa ttacacttag cccctcgcgg atcctatccg
     3181 ggggcataca atacggaacc aagacctggt gttctgtgat gaacaattga ctgctcatac
     3241 agcaccaggt ctgtttatta aaaagccttc agttctccca cctcttgaac atcaaccgta
     3301 accccttcca atttggtggg gatgacatcc tcgggggcga gctgggttaa gggtactttt
     3361 ttattgacca tgaccaccaa cgccagctca tccgttcgct tgccagcatg atggcgatag
     3421 cctaaaccca cgccgactac attggctttg gacatcaggg ctttttcata tgcgcggcgg
     3481 actgagcgga ttttttcagt ttgggcttgt aaatcttgca taataaactc aaattgtgat
     3541 acttaggcta tccagcaccg cctgaatagg gttgatgatg gtggtttgat ccgatccggc
     3601 gaagagcaaa ccgatagctt ttaaggcatc tccagcaacg accaaggatc ccgaatcgcc
     3661 gccttgcgac ataggtcctg tgacaatctg gttgtcgaag cgggctaccc gtcctgtgcc
     3721 ataacttacg tttacggtgg catctaagac ggtaatctca cctgtggtga agccggtggt
     3781 gcgcccggat ttacgcactg acatgcccag ggttgcttcc atggtgccgg agaccacacc
     3841 aatattcagg atttcatctt taatatcgtt atcgctcacc gggcgggcga cggcgccgtc
     3901 cactagatta gcggcctgcg ggttggcata gatagcttgc acgcggtgtt tggctcccat
     3961 cgcgctggcg atggcattgc ctaactcggc aaaagaatta gccaggctgc aagtgcctgg
     4021 ttcctggctg aaatcgattg gatgaaagcg ctccaggtga gcgataacat ccgaagaagt
     4081 gctgcctcca tccgtaggac caggctgaag gatgggatcg ccgacagcgg cgtcgttgct
     4141 attcgccagg acatggttgt tggataagat caggcgatct ccgcttgccc ggtcgcgaac
     4201 tacagcccct agcgtcccag cagtaatttt atagtggccg atgctcaccc cacctggggc
     4261 aggtcgccag cgggaagtcg gggtttgtag cgccctcaca tcacccactt cgatcacatc
     4321 ggtcaatatt ccatcatact gtttggatac aatcgctttt ggtgataggc catccaccgg
     4381 cattttctgg cggaccaatg taacaatgca catctcgctg gtgcgcaccc ctttggatat
     4441 cttatacccc gttccaactc caacaacatg cggtttggaa agaatttctt ctttatatgc
     4501 atccttgact ctttgtattg cggcacgctc ggccatttct tcctccttgc ttgagatttt
     4561 attattaaca atctgtcaat tcgtgatgcc cgggaaatct atcttattaa agataatcat
     4621 aatcgaaaag tcaaaaaacc caaaataact tgaagggatt ctatttagag cattttaccc
     4681 ccactggggt ttgaatttag ggtattttgg aggtggggtg ctcgaccaca attccccaaa
     4741 cacccgtttt gtgggattgc acctaatgta aggggaaatc tattctctta agaataggta
     4801 aagaggggtc acatagattg agcgatttgc ccggcttttt caagtgctgc cgcaaagcca
     4861 agcagggcgc gctggatcat atcgtcactg gcggtcagag aaatgcgaaa atagcccggc
     4921 atttcgacca cagtccctgg caggcagaat atcttgttgg attggagcat ttcaacgaaa
     4981 gcttcatcgt ccttccaagg cgagcgtggc agcaagtaaa aagtgccgtc tggaaaatga
     5041 agctcgtacc ccatattcct caattctcca accaaataat cgcgcttttg ttgtagatgg
     5101 tcaacgtcga tggatagttc gttcaaatct gccaaggcgt gttgaagcag ggcgttggga
     5161 aaagcgtacc cattgacgac ttgagagatg aacagcgctt ctcgcatttg tccgcgatct
     5221 ggcatcttgg gagggaggac aatatatcca atgcgctggc ctggggtgag caaggtcttt
     5281 ccataggtgt agatgaggaa agaatagggg tagtattgcg ttgggctggg aaattgaatc
     5341 ccatcgtaga tgatgcggct ataggattcg tcagagagca gataaattgg tcgtccgatc
     5401 tttttctgag cagcatgaag tgcgtcggct aattggcgca atgtttgggg agggtatatt
     5461 ttcccggtcg gattattggg cgagttaatg attatggcgc gcgttttggc agtgatcgcc
     5521 gcagcgatgg catcaatatc caggtcgaag gttactgaat ttatccgtac ccgaataggc
     5581 acgccattgt gagagagtat cattggctca tagaagaacc agggcgggct gataaaaatc
     5641 acttcgtcgc ctgggtcgag aattgcgcca agcgtaacgt tcagcgatgc aaaagctcca
     5701 gatgtgagga aaatatcctc tgggctataa cgaattccac gccaacgata taacgattcg
     5761 tccaaggcag cgattgctgc tggctcgctt gttttgtagg caaaccaatc cttgttgccc
     5821 ggggtaatcc agcgttgcaa ggcgtttaca tattctgcca aaggcatttc ctggggattt
     5881 cccaaagcaa agtcgcaagc caggggatcg tcattacgat gttcaaaaac aggattggta
     5941 aagaatttta tgaacggctg agatgacgtc ataacattat ttatgcgctt agaaatgctg
     6001 ttcgatcgca tggttaatct cctatcaagg gttgataaca taatggttgt ggagcgtatc
     6061 ttgttgtagt aagcgacgct cactttctgg acttcggttt gttatccaga tttgtttaca
     6121 tctacaggtt ttctatccgg gatcctgaat tcagctctgt tacctgaaat tggtcatcca
     6181 cgaggctgac tttaccccgc ccaatgactt gccatccacg attggttgtt tgcacaaagg
     6241 cggtctgttc gtcgatcccg atgacaaaat gatgcggcgg gagcagtcca ctttttagcc
     6301 agcgaaggga gaataattgg tttgcatggg gccagcacat gctgcggggc acaaaaccca
     6361 agcattccag tgcaggtggc aggggtaaat cccactcgga aatcccagct ccttgagaga
     6421 ataacttggt gacagccgcg tcaagctctg gggtgatcac ccaggattga gcacccagca
     6481 tcatggcgcc ggcgctggcg ccagcgatca acgcgccgcg ctgccaagca gcttcaagcg
     6541 cttgctgcgc tcgggtgcca cgcaggatgc gcatgccgac gtgtggatat ccaccaccga
     6601 tgtaaatcca atcagcctcg atgatcaggc gtgcgttctc aggatcgtcg gcgctgggtc
     6661 gatcgacaat gcgcaaaggt ttaaccttgg ctcccaccgc ttcgagacgc ttagttgctt
     6721 cgtcgcacca gtaggtaacc gtatctggac cgtcgtcggt cgcacatgtt ggcagaaata
     6781 ctacttgaac tggcttgcca tccttctggc tacggttgga gaaagacagt tctagcaggt
     6841 tagcgtgaac atcctcgaag ccatctgcaa attcttcgcc tccaattaag gcaatcattg
     6901 atttcatgac tatttagcgc aatccagcaa atagatcggt ctcgattttt cgcccgccat
     6961 ttaccaagct ataagcgcgg taataggtgc gctcgctcca caagcgcctg tgctgttctt
     7021 cggttattcg ttcccggtca ccaagccctg caatttggga tgcgtggcat tggactgctt
     7081 gccaggtaga aggccaatat ttctccccat cgatgtgagt ggtgatagcc caatcagacc
     7141 aggctactgc ctgtcgctct ttaccgtcga ttgtcatgcg cagatcgcca aatatctgag
     7201 tatagatttc aaacaactcc tggttgtccg ccgtgtaata cagctttgag acgcggtggg
     7261 gctggagttg attctttaca gggtaggtag ggtctgctgc actgacgagt gcagcagttg
     7321 tgaattggca gatagcgata tggtcgggat gcccgtaagc tccatcgggc gggaaggtaa
     7381 ttacaacgtc agggcgaact cgacggatgc tttcgacgat cttaccaacg gcttctgttg
     7441 gatcagcctt gtcaagatct ccatcgatat aatccagaaa ttccacctgc tttatgccca
     7501 gcgtttgggc agcgcagcgt agttcggctt cgcggattcg ccccagagct tctttaccgg
     7561 gaaaatcctc tctggtgcca aaccaacccc gttcgccgcg cgtggctgtt accagataaa
     7621 cctcgatacc ttcatcggcg tattttgcca gggtactacc aacgcctaaa gtttcatcat
     7681 ctggatgagc catgatacac attaatttaa gagtttcgtt cataatttgt agccccttat
     7741 ttgacaaaac agaaacaaat atacgattaa agattatatt actctaatta caattatttt
     7801 tcaaccattt aacagtctat ttcacgtcaa atccgagcgc atcgacaaaa tatctggttt
     7861 gccagtggga tggctcaatc ccccagggca attgcatatg ccctgagggg caggcgcaat
     7921 cccgcaaaaa gagtgtttgg ggtgatttgg cgggggcata cccccaccaa atcaccccat
     7981 tttctctcct cagttgtggg aaatcgccca aatgcgatta ccctactcaa tccccagctc
     8041 ctttcaacaa acatattttg ggtgtggtag aatccgtcac cagatgtcat tggtaataat
     8101 ccaaatcatc cgaggggtaa tatgaaacat catactggct cgattgaggt gattaccggt
     8161 tccatgtttt gcggtaaaac cgatgagttg attcgtcggt tgcgcagggc aaccattgcc
     8221 cggcaaagag tccaggtctt taagccagtt attgatgacc ggtattccgt tgaaaaagtt
     8281 acctcgcatg caggcagtga gtatgctgct cagcctttac agcatgcggc agaaatccca
     8341 tccttaattg agccggatac cacagtggtt ggcatcgatg aagcgcaatt ctttgatagt
     8401 gatattgtcc ccattgtgca atccctggca gaccggggga ttcgtgttct cattgctgga
     8461 ttagacacgg attttagagg cgagcctttt ggcccaatgc cattgcttat ggcggcagca
     8521 gagaacgtgg ataaactcca cgcgatatgt atgatgtgcg gtgaagcggc tagtcgcacc
     8581 caacgtctgg taaatggcaa gcctgcacac tataatgacc cggtggtgat tgttggtgct
     8641 tcggaattgt acgaagctcg atgtcgtgag catcatgatg ttccaaggaa ctaagcttgg
     8701 gatgatgggc atcttatgca gggctataaa gaatttttag atgagatatt aatcactgag
     8761 gaagcactac agaaccgcat tgcggagctc ggggctcaaa tcagtaaaga ttacgagggg
     8821 aagcagccgc acttgatctg cattcttcgc gggggtgtga tggtcttatc tgacctgata
     8881 cgccacctca ccgttccaaa tacaatcgac tttatggcga tttcatctta cggggtcggg
     8941 gcgcgtcaat ctaccgggaa ggtgcgcata actctcgact tgcaggaaga catcatcgaa
     9001 cgcgatgtgc tgctggtcga agacattgta gatagcggct acaccatcgc cacagtactt
     9061 gagtttctta aaacacgcca cccgagcagt ttgcgagtct gtacattgct gaacaaggcc
     9121 gagcgccggg aaatccaggt gccaatcgat tattgtggat ttgttattcc ggataaattt
     9181 gtgtttggtt atgggttaga tttggatgag ctgtaccgta atttgccgtt cattggcacg
     9241 gtgaatcctg agcgttacca tccgcctgtg taatcaaaat gccgcgccag ccgaagtccc
     9301 ctccgggtaa tcatgatccc tatatgggtc gttgggttgc ccgcttgggt gagcgtatca
     9361 tcgggcaggg aggtactccc gagcaagcgt tatgggcggc aagagcggcc cgtagcaaag
     9421 agaatcccca gatagattac atacccacag tgcaaccatt agtattctcc gcgcgattag
     9481 agccaatccg tagccttatg cccgttgata tgccggcata tctggttggc ggcgcagtgc
     9541 gggatgccct cctgatgcgc acaacccacg acctggactt tgttttacca tccggcgctc
     9601 tgaaagcagg gcgcgcggtg gcgaatgcct tacggggcgc ttattatccc ttggatgcgg
     9661 tgcgccagac tgcgcgagta atcctgtatg ccccagatgg gacgcgggag tttttggatt
     9721 ttgctgtaat tcgcggagag gatttagaga gcgatttgcg cgctcgggat tttaccatta
     9781 atgccatggc tattgacttg cgccacccca cgcaattgct agacccgctc ggcggtgcgg
     9841 cggatttact tgcccgccaa ttgcgtgcct gctctcctcg ggctttttta gacgatccag
     9901 tgcgtatatt acgcggtgta cgcttggctg ccgagttgaa tctgaaaatc cagcccgaga
     9961 cgttgaaaat aatgcgccaa tccgtggggg agttggagcg agtctcgtcg gaacgaaaac
    10021 gagatgagtt gttccgcatc cttgatggcc cgcagccagc caaggcactg cgcgcactgg
    10081 aaatactcgg cgcgctggtc tacgtgctac ctgagttggt gagcctgaaa aacgtaatgc
    10141 tagcaccgcc tgtagataca aatgtgtgga cacacacttt ggatgtggtg cgaaacctgg
    10201 cgacccttct cgatgttctt gatctgaaat acaaacctga taccgccgct aacctatcga
    10261 tgggtttggt atctatgcgt cttgggcgtt accgagagca acttgcggag catctgaaaa
    10321 gctccattac gccagagcgt cccctgcggt ctttgctctt tctggcagcg cttttccatg
    10381 aggtagccca gtctcaaatc ccccaggtgg acgagagtgc gggcgatatg attgacaacg
    10441 atatcgcggg agctcagatg gtcgaagaac ggggagaggc actccatttg agtaatgatg
    10501 agattgcacg tctaaagata attgtacgct ttcacaggag gacgatcctg ctggcacagg
    10561 aagaagaccc cccatcccgg cgagctattt accgcttctt cagagatgtg ggtgatgtcg
    10621 gcgtggaaat cagcctgcta tcccttgcgg acaccctggc aacctatggc ccagctccgc
    10681 accaggatat gtgggcgcgt cacatcaata ccgtccgcat gttgtatgaa gcctggtggg
    10741 agcatcatca agaatacatt tctccccctg ctctgttagg tggccatgat ctaatagatg
    10801 agcttcgcct caaacccggc ttgcatatcg ggcaaatatt agaggcgata aaggaagcgc
    10861 aagccacagg tcaggtgacc gatcgtgatg gtgcgctaag tctggctcgc tcctggttgg
    10921 ctgaacacag tatcgattag gggtatatct cagagagcat cggctcgtag aagcaacgac
    10981 aacccagcga gtgtgagcaa ccctcgaccg gtagcttggg tacttgatct ttggcgtagg
    11041 ttccctccac ctgctgacaa gcaggacagc aggtgtcggc gactaccagg cgcaggttaa
    11101 gtacccttgg attggcgcgc agtctcttca aagccgcgga tattttagag aattcactca
    11161 gatctgcttg acaattactg caataagcag ccgaatctgg agattgggca ttgcattgag
    11221 aacagatttg cacgtcactg cctccgtgaa aattttcgct aattgtaatc gaaaataagg
    11281 cagttagata tgtcacccca aacactctct tcagtggtgg gaaatcgccc taatacggat
    11341 gccctacacc tttttaggct tatctgtcga gatccttctc tgggctttat ttcgggctag
    11401 agtggaatca tcttttggct gggatggata tttggcagca tgtaaggcgc gaatgtcttt
    11461 ccccctgcga ggctccgagt tgcggtgcaa cgtgcttcca gtagagggtt gtgctttctg
    11521 gctgcttttg tcagttcggt ggttggcatt ttcttcagct cgagcacgcc agaccagtct
    11581 tttcagatgt gcgatcaaga cgctactggt catgggcttg agcagaaaat cgtcggcgcc
    11641 ttcatccagt gccttggcga ccatccccgg tctgctgact gctgaaagta ccaggatagg
    11701 cgcctggcta aaggagcgta tctgcttgca gacttccccc ccatccatat cttccaggga
    11761 tagatccaag atgatcacct ctgggctcag gtgcttggct gattcgatgg cttctcgtcc
    11821 cgaatgggat actttaacat caaatgcatt tggctctagg attaaccgca acatttctgt
    11881 catggcggca tcatcgtcga tcaccaatac tctggttaat gcagcgttat tcacctagac
    11941 ggttttactc ctcaaatgaa cgaatcggga tcaggaaaaa attctcctga ttgcttcccg
    12001 gctgtctgct ccaattatgc ctgaaaacag cggtgccaca ccttacaatc acttaacagg
    12061 tcggtctgcg aaaatgcttc tgacttgcgc cctggaagat tcggagatat gattatatcc
    12121 cagatgagct cacaaataca ctgtcctcag tgcggtcagc cgactgaatc ttcttcgcgt
    12181 acttgtgaaa aatgtggagt agtcctggca attgccgcgg tgttggccga acgcgagatt
    12241 ctgcctcacc ggatagatgg tagcttagcg gtaaccccgg aaatcctggt gccacgtctc
    12301 ggtgaatatt tggttgaaaa ggggaagttg acacgtccca atctagaacg tgccctggat
    12361 tatcaaaaga agaaatcccg ttctggaaag acgctattat tgggacaagc cctgcgcgag
    12421 ttagaattga tcacagccga ggaattagat caggcgatta ccgagcagat tcttaatttg
    12481 caagtggcct taagacgctc caatcaggag ctggaagccc gcgtgcgcga gcgcacaatc
    12541 gaattggagc gggcgctcga aatgctgggg gagctcaacc agttgaaggc taatttcatt
    12601 gccaacgtat cccacgaatt gcgaactccc ttgactcata tcaaaggata tctcggagtt
    12661 atggctactg gtgagttggg accgatttcc gtcgaccaag ctgatgcctt gcgcgtaatg
    12721 cagcaagctg aagcacgttt ggagagattg atcgaagatt taatccaatt tgcgctcata
    12781 tcgcgcggtc agcttacctt gagcctgtca catgcgaaca ttgccgaaat ggttgagaat
    12841 gtggtaaacc aagtgcagca aaaggctaat ttggctggca tcaatttgga gacagatatc
    12901 tctgaaggtt taccgctcgc ggtctgcgac aatgacaaaa tagcctgggt aattgggcac
    12961 ctgttagata atgcttgtaa attcactccc agaggtggtc gcgttaaact ggaggtagac
    13021 cagtccgata aatttgttaa aatagcagtc acagacacgg ggattggcat tcctgcccat
    13081 cggatgccag agatcttcga gccatttcac caattggatg gttcgtctac ccgtcagttt
    13141 agtggaactg gcctagggct ggcattatct cagagaattt tgacggccca tggcacacaa
    13201 atcgaagtcc aatcacaaga agaaaagggg tctcgatttg agttcttgtt gccactcgca
    13261 gaagttgcga tgcaaatgta aaaccagata tggttgacga gaccagtaac ccaaccgcac
    13321 ttcgccctga agaactagag gcggtatact ccatcagccg ggctgtagct ggggctgtag
    13381 atttggatac agccctggat gaaattattc gtttaacgcg ctccgtattt atttttgata
    13441 acatggtgct ctataatttg cggagcatcg acacgcttga gcccgcatat gctcgcgcca
    13501 ttggccgggg gcgctcccaa gaggccgagc tggtttgggg cgaggctacc gccttggagg
    13561 caattcaaaa aggtcaggta atcattctcc aagaggagtc gggggatcaa gaagatcgta
    13621 ccggagtgcg tttttcactc ggtttaccac tcagatcagg caaagatatc aaaggagccc
    13681 tggtgtttat ccgttttggg ggtccaacgt ataaacctga ccagatacgc ctggcagagt
    13741 ttatcgcaat gcacgtcgcc cagttgttag agcacggtca acttgtgcag cgagtggtca
    13801 gcctggaggc agaacgccgt ctggaccggt tacaggaaga tttcatagcc accgtatctc
    13861 acgaattgct tacaccatta ggtttcatta agggttacgc caccacgctg ttgcgtgaag
    13921 acgcggtctg ggatgatgca acgcggcgtg aattcttgag catcattgat gaagaagcgg
    13981 atcgcctgcg agaactgatc gataacctga tggattcatc caggctacaa gccggcgcgc
    14041 tccgcatgag cttccagccc gtccgcatgg atacctttct caaagatctt agtttccgag
    14101 caaagtctag ggatgagaag cagaaaatca atctcgaatt atattcccca gatctgatca
    14161 tccaggttga tccagcacgg ctggctcaag ttttcgataa cttgctgagt aacgccaata
    14221 agtacgcacc aggtgcggct atctcgatta cgctcgatcg agttaatgag ctggctcata
    14281 ttcgcgtcaa agatgatggc ccaggaattg atcctgagca tttaccaaat attttcaaga
    14341 ggttctatcg tgtacccagt aacaatacca cggtgcgcgg cacgggacta gggctgttca
    14401 tttgccggca aataattcgc tcccacggcg gaaatatcag cgtggaatcg aagcctggtg
    14461 atggaacagc ctttgatata tatttaccca tagatcgtgt tcaaaccgaa gaacaaattt
    14521 cactgcggga gagtaactga tgagtacaaa aatcctggtc gttgacgatg aaccacgcta
    14581 tttgcgtctg atcgaagcta acctggcatc ggatggtttc acggtgatca aggcgatgaa
    14641 tggacaagag gcagtggaaa aggtggcttc ccaccagcca gacctggtcc tattagatat
    14701 catgatgccc gttctggatg gatttggcgc gacagaacgt atccgggaat tctctaacgt
    14761 tcctatcatt gtcgtcactg ctcgaggaga agagggcgcg cgcgtgcgtg gattggattt
    14821 gggagcagac gattacattg tcaaaccctt ctcagccacc gagttgctgg caagagtgcg
    14881 cgcggtgctc cgccgggctc aagtctccgg tggtagtttt caacagtcaa tcttcaacca
    14941 tggcaatctc aaaattgatt ttgcccgtgc agaggtattc cgcaatgatc gcgtggtgtt
    15001 cttatctgct acagaatatc gcttgctgtt acaattcgcg cacaatcttg gacaggttct
    15061 cacctcggag caattgttga ccaatgtttg gggtcaggaa taccgtgatg acaaggagat
    15121 cttgtgggta agtatctctc gattgcgcca gaagctagag gatgatccca agaatcctat
    15181 ccacattgtc actcgctcgg ggcttggcta taccatgccc cccatgcaag actaaatgac
    15241 agataaggca aatatataat ggtaaatccc ctttcaatat ctcttcgttc aaagaaactc
    15301 ggtgtactta tccgcgacgc ccgtcttgca gcgcgtaaga gtatcgaaga gtgttgtgca
    15361 gtgattgagg tatctccaga agaatttgag gcctatgaat taggggacaa atctctatcg
    15421 cttcccaagc tcgaggtgct cgcattgtat ctgggtatac ctctggatca cttttggggt
    15481 agcaaagcga gatcggaaga tgcaagcccc gcactggatt ttgatgtcaa acaactgctg
    15541 cgcgtacgcc agcgcatgat tggcgcgctg gtgcgtcaag cgcgaactca gggcgatatc
    15601 tcgctggaag aattgtcaca ggaagtcggc attgcccctg atcaacttga agcctgcgag
    15661 aatggtgaag ccgagctgcc tctaccgaca ctcgaagcgt tttgtgcaag attaaatcgc
    15721 ccaatcgaag aattttttga taaacaaggg ccagttgggg tttggattgg acaacagcaa
    15781 gccgtccaag acttccttca acttccagat gagatacaaa catttgtggc taagccgatc
    15841 aatcgtccat atctggagtt ggcacagcgt ctgagtgaga tgtcggtcga taaactgcga
    15901 gctttaggag aggggatctt ggagattaca ttatgaggga cagtatgaat gagcagattt
    15961 ctgtcaacca gctaatcagt caaggaaaat ccgcctatca acagggggat ttcattgcag
    16021 ctgcacgtgc atttgatgct gcaggagata gctacagtgt tagcggagac gagctaaatg
    16081 ctgctgagat gcgcaataat agcagtgttg cctatttgca agctggcgaa gctcaagctg
    16141 ccctcgttgc agtggaaggg acgccagcaa tctttgcgac cgctggcgat ttacgccggc
    16201 aggggatggc gctgggtaac ttggcggcag ctcttgaggc aatgaatcgc ctagaggaag
    16261 cgactaaagc ctacctccag tctgctgaat tgctcgaatc aattggggag aaagacttgc
    16321 gggcttatgt aatgaagtca ctctcagcat tacaattacg ctcgggtcgc catatcgagg
    16381 cattgtctac gatgcaagct gggctggatg ggatcgaaca cccaaagccc cagcaacgtc
    16441 tcctgaagcg gttattgaat gtcccattta aatttctggg aaagtcatca gactaaacgt
    16501 tatcccgctt cttgtaatcg cttcattcca gctttcaaag catatataat agtcatcgat
    16561 aaagacggcg ttcatgatcg ctaatctcct gcagcttact gttcggcctg tatcagataa
    16621 ggatctccag cgattggcga acctgatcca ttttgaggcg cttgttcacc ggcatttgga
    16681 ctggcgagca ccgctggatt gggtcggcta taacccttat ctggtcgcag aacacaatga
    16741 tgagatcctc gcggcactgg cctgcccacc agatcctccc tctgtggcct ggattcgttt
    16801 attcgcgaca tcatcggaaa tctcggtcaa tctggcgtgg gaggagctat ggaacgcagc
    16861 ctgtgaccaa atatgtaacc tgggtgatac agcgttagtt gccgctatcc ctctgcaaaa
    16921 ttggttcaga aaattgctcg aaaaaagcga atttgtgcgc tcacacaacg ttattgtgtt
    16981 gagctggaga cgggctccaa gacctccagc atcaagagct ctccctgggt tcatccgcac
    17041 aatggagcta agagacatca atgccgtaga ggaattggat aaggctgcat ttggcctaat
    17101 atggcagaat tcttattcca gtttagaagt ggcgtttcgc caggctgcgg ttgccaccgt
    17161 cttggataat gacggagaga tcattggcta ccagataagc accgctaccc ccgtaggagg
    17221 tcatctggcc cgcctgggag tattgcctgg gtatcaagga caaggcattg gctatgccat
    17281 cttggacgat ttgttggtgc aatttgagcg ccggggtgca cataatgtta cggtgaatac
    17341 gcaaagtgat aacctcaatt cgctcgctct gtataaaaag gcggggttca agcgtacggg
    17401 tgaggaatac cctgtttatc agtattcgcc atgactgaat tgacaaattg ttctggtcat
    17461 ggcaaaattt ttcaaatcta gagattatgc agttggaacc tttcctataa ttgcctaagc
    17521 tatagaaaca cctcaaatca aggagattga atggctaaga agcaacgacc tggtacaccc
    17581 agcaagcgtc aaatcatccg cgaacaacgc atgaagcgcc agcagcaaca gcgcctgctc
    17641 accattttgg tgattgtggt agctgctgtg gtgatagcag ggctattgat ttggccttct
    17701 ctacgtccgg ttggcgagat taaaaccatc acgccagtcg agcgtccaca agaagacgga
    17761 actgcattag gtgatccaaa tgcaccggta ctggttgaag tatgggaaga tttccagtgt
    17821 cctgcctgta cgggttattc ccaacaaatc gaaccacagg tcgtggacaa ctacgtcgcg
    17881 acaggtaaag ttcgctatgt atttcgacac tacccgttca ttgatgatcg cgtggcgacc
    17941 aaagaatcgg atcaggctgc caacgccagc atgtgtgcag cggagcaggg gcgtttctgg
    18001 gattaccatg atattgtgtt tgctaactgg aacggcgaaa acgctggggc ctacagtgat
    18061 aaacgcctga tagccttcgc tgaaagcatc ggccttaaga tggacgattt taatgcctgt
    18121 tttgacgcca accgctacaa agatcagatc gataaagatt ttgcggatgg cacagatgcg
    18181 cttgtgcagg gtacacccag tgtgtttgtg aacggagtaa ttgtgaagcc cggctttgtt
    18241 ccctcttatc cagaaatcgc tgcggcaatt gatgcagccc tggcatcaca ataggattgc
    18301 tctttccggc
//
LOCUS       DBNGSX010000083         8716 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1391342, whole genome shotgun sequence.
ACCESSION   DBNGSX010000083 DBNGSX010000000
VERSION     DBNGSX010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8716)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8716)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8716
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1391342"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="AC16CB_02760"
     CDS             <1..260
                     /locus_tag="AC16CB_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HIR3341418.1"
                     /translation="FELCNAFTELNDPLDQEARFREMGRDYAADDEERHPMDDDYLRA
                     MRYGMPPNGGFGMGIDRLTMLFTDSANIREVILFPHLRERD"
     gene            complement(281..514)
                     /locus_tag="AC16CB_02765"
     CDS             complement(281..514)
                     /locus_tag="AC16CB_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341419.1"
                     /translation="MKKFTGPRAHGAKLADFEMAKLWREIRYAPPSGAHVPQYRSGTR
                     QAGADIPLLVVTTIQPGPLLPVNRIAKLGTAVK"
     gene            612..1043
                     /locus_tag="AC16CB_02770"
     CDS             612..1043
                     /locus_tag="AC16CB_02770"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3341420.1"
                     /translation="MIKVKGSILINAPVEKVFAFMDDPCNLPEIWPSMIETHAKGPSS
                     AGGQDFEWEYKMAGLRIKGESEVVERIPNRKMITKSKKGIETTFVWDYVSEAGGTRLT
                     VEAEYNIPVPLVGRLAESVIVKQNEYEANMLLENLKTRMEG"
     gene            complement(1228..2775)
                     /locus_tag="AC16CB_02775"
     CDS             complement(1228..2775)
                     /locus_tag="AC16CB_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR3341421.1"
                     /translation="MSNDQAASKPSLWHRASLLIYPFLLVYYPILALRAHNIVYVNLP
                     SILRSLLLVTAGTAILIAITHLFVRNLAKTSIIVSLIGMFFLSYGHIYIKIEETLGSP
                     VRHRYLVAASFLILLLVTILILKKDQVARVLAQFLATTSIVLVAIVLFQTVNYDIGVY
                     RAEAASAQEEAPVAPVQADEKLPDLYLIILDAHTRTDVLEARFGFDNSSFIQKLNDMG
                     FYVASCSQSNYASTKLSLTSTFYGDYIQNFVEEGEILPSLKRSALNQTLKSLGYKIFA
                     FENRASGHFDLKEDVLLSRNQMAFGKFDFIGGISEYEKMLIDTSFLRFVVDTELIPGF
                     DNTRTQEWELWEHYYQTNYILAELEKVPDSPGPKFVFVHIMVPHSPFIFAPDGSYQHN
                     NSPIDGYRANTEFIDSHLPPVLQTILEKSNPTPIIIVMGDHGPSTRKTITKEMRMATL
                     NAYLVNEAAKAQMYPAMTPINATRIILNAHYDRALPLLEDVSYYAYKGSQLPDAEIIT
                     NNCPPTP"
     gene            complement(2923..3792)
                     /locus_tag="AC16CB_02780"
     CDS             complement(2923..3792)
                     /locus_tag="AC16CB_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR3341422.1"
                     /translation="MSNSPRKVKQVIEPQYVIEGAGVLLRRSFGPNRTNLFDPFLLFD
                     HFAFNDPIEGPIRGFPTHPHRGIETVTYMLEGNVRHRDSLGNTGIIGPGDVQWMTSGR
                     GILHEEMPRRGPNGVIYGFQLWVNLPAAQKMSQPRYQEVNADSIPTAEKQGAKVRLVA
                     GEFGGVKGPVTQIAAQPLYMDVTLDPGAEFYLPVPVGHTTIAYLFEGEGLFGLDAEGQ
                     GEFTPAVRMIEFEDGDHVRVQAGQGSHARFMLMAGAPFKEPIFPYGPFVMNTQEEIQQ
                     ALNDLRNGTFVQN"
     gene            3974..4570
                     /locus_tag="AC16CB_02785"
     CDS             3974..4570
                     /locus_tag="AC16CB_02785"
                     /inference="COORDINATES: protein motif:HMM:NF019959.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3341423.1"
                     /translation="MSPRPDVSLERKHQILNAAEEVFSRKGFDEARIDDIAEETGLSK
                     GTIYWYFDSKDDIIIGILDRIFQREFKHMESLNLLELPANEAVWQYVKIASQDITNML
                     QLMPIAYEFLALAFRNQIVQKALQQYFGRYMDILIPIIQGGIDSGEFRTVEAREVAIA
                     IGAIFEGTILLWVYDQNMVEPERHISSGIRLLLEGIRA"
     gene            4626..4898
                     /locus_tag="AC16CB_02790"
     CDS             4626..4898
                     /locus_tag="AC16CB_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341424.1"
                     /translation="MLQVIILVLAMVLVGLLVGWLAGLIWKNQRPIGVSGDYMVAIIS
                     AIVIGLIDWFVIPAMGFSDTMKYLGVAFEPLLGALLVLWIIRKAKQ"
     gene            4968..5837
                     /locus_tag="AC16CB_02795"
     CDS             4968..5837
                     /locus_tag="AC16CB_02795"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3341425.1"
                     /translation="MDGLDLSVQPGELFGLVGPDGAGKTTTLRLLAGLLDVSGGEATI
                     LGFDLKSQPEAIKPRVGYMAQQFSLYAELSVLENMRFFAELFDVSAQDLSERMQGLLV
                     FAGLTEFQDRRAAHLSGGMQKKLALACTLVHRPEVLLLDEPTTGVDPISRREFWNILT
                     ELHLDGTTIMVSTPYMDEAERCSRVGLMFTGKLVVCDTPQNIRNRLEGDVIELYSEDW
                     QSARERVAGLPGVREVQTYGESLHLVVDAGEKRLPEIESALKKAGVDLRSMRIAPPRM
                     EEAFISLIRKMEK"
     gene            5837..6970
                     /locus_tag="AC16CB_02800"
     CDS             5837..6970
                     /locus_tag="AC16CB_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3341426.1"
                     /translation="MSISHIWAVTRKEFLHIIRDRSTLFLVLFTPTLVLLLMAYALTV
                     DIKHVPIGILDYDRSPTSRAFIQQITAGQDLDIYAYAENMDEIDSMLMHGKIKAALVI
                     APGFSSELQGLKGMPIQVIIDGTEPESGNFAMQHITRRAEEFIDQVLTRRLQAAGVSL
                     ASLQPLDLRVHTWYNPDLKPRNDLIPGLISMVLGFPALSVALTLAHEREHGTLEQLLA
                     TPITRGELLLGKIVPYLAVGLVNVVMIPALAIAWFQISFNGNFLLYFGLSAIFLFAVL
                     SMGMIVGVFMRTQAAALALSFLVIFFPGFFLTGIFFPLASMPEEVRMEAMMLPGTHYA
                     VITRGVFLPGVGLEVLWPYAVMLIVLGLAFTGLAALFFRKKLG"
     gene            6974..8098
                     /locus_tag="AC16CB_02805"
     CDS             6974..8098
                     /locus_tag="AC16CB_02805"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3341427.1"
                     /translation="MLRRIWILVIKEFIHLRTDWWLPAFMLLGGAMELLLVGWATSRP
                     ITNLPLMVLDQCNSPASRSLLVALENTGTFQLQEQVSDMVTIESALEQGKINAALVIP
                     PDFSEQINSSYGRPTLLAILNGAESTPAMAALRAIDGVSREVDKRILIQRLGLSADEF
                     SDFNLSLRVWFNEELSEALYTTPAELGLMLEFTVLLFAALAFSRERELGTLEQLLVMP
                     FSSLEIIIGKAIPVIIVGFTDFLLMLGMVHFAFGVPVRGSLPLLLVLALGYLLVELSK
                     GMVISVISRTQHQAFLLVMLLGMVDFMFTGYAAPVDGMPKALQYFANLIPAHHWLSIL
                     RGILLKGAGLDVLWQHVLTLFILGLLIGTFSWRFVRRALD"
     gene            8103..>8716
                     /locus_tag="AC16CB_02810"
     CDS             8103..>8716
                     /locus_tag="AC16CB_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3341428.1"
                     /translation="MLERTPAIVAHKLTKRFGDFTAVNDVDFEVQRGEIFGFLGPNGS
                     GKTTTIRMLLGLMKPSAGSVEVLNMPVDGDTQKIRPRVGYMSQRFSLYNDLSVLQNLR
                     FYGAAYGLSDAELEVRIGEALVMCGLEGREDSKTKDLSGGWRQRLALSAAILHRPEVL
                     FLDEPTAGVDPVSRRVFWDLLYALVVQGVTVFVTTHYMDEAEHC"
BASE COUNT         1984 a         2111 c         2479 g         2142 t
ORIGIN      
        1 gatttgagct gtgcaatgcc tttaccgagc ttaacgaccc gctggatcag gaagcccgct
       61 tcagagaaat gggccgcgat tacgcagccg atgacgaaga gcgccacccc atggatgatg
      121 actacctacg cgccatgcgc tatggcatgc cacctaacgg tggttttggc atgggcatcg
      181 accgcctgac tatgctgttc accgatagcg ccaatatacg cgaggtgatc cttttcccgc
      241 acctgcggga gagggactag agcgaaaatt tttttaagtg ctacttgaca gccgtgccca
      301 attttgctat tctgtttaca ggtaagagtg gccctggctg gattgtggtc acaacgagca
      361 aaggtatatc agctccagcc tgacgggtgc cggagcgata ttgggggaca tgggcacctg
      421 agggcggggc atatcgaatt tccctccata gcttagccat ttcgaagtca gccagcttag
      481 ctccgtgagc tcggggtccg gtaaattttt tcatatggac ctatcgggtt ttcactgatg
      541 tcaagcaaac atccactgga tggtttgtcc atcgtattcg tatccaacta aaaaaagcag
      601 gaggaatgac aatgatcaaa gtgaagggta gcattctgat caatgcaccg gttgaaaaag
      661 ttttcgcttt catggatgac ccatgcaacc tgccagaaat atggccgagc atgatagaaa
      721 cacatgcaaa aggaccttca agcgctgggg gtcaggattt cgaatgggag tacaagatgg
      781 ctggcttgcg cattaagggt gaatcggagg tcgtggagcg catacccaat cgtaaaatga
      841 tcaccaagag caagaaaggc atcgagacaa cattcgtgtg ggactatgta tctgaagcag
      901 gcggcacccg cctgaccgta gaggctgagt acaatatccc ggtcccattg gtgggtaggt
      961 tggctgagag cgtgatcgtc aagcaaaatg agtacgaggc caatatgcta ttggagaatt
     1021 tgaaaacccg aatggagggc tgagagccgg caaataccaa tctggaagac catctgatgt
     1081 ggaggccgcg ccccgctgtg ccgactgatc ttccctccgg taaaatttag gtgggtggct
     1141 cggtggaaag tatgccagcg gggcgcaggt ggtgattaga tcaagcgccc tgggttggtt
     1201 tgtcaaatgt atatagggta aagggggcta tggcgtaggt gggcaattat tggtaatgat
     1261 ttctgcatca ggcagctggg agcctttgta agcataataa ctgacgtctt caagaagcgg
     1321 aagagcgcga tcgtaatgag cgttcagtat aatgcgcgtc gcattgatcg gggtcatggc
     1381 gggatacatt tgcgctttgg ctgcctcatt gaccaggtag gcattcaggg ttgccatgcg
     1441 catttccttg gtgatcgttt ttcgggtgga tggcccgtga tcgcccatga cgatgatgat
     1501 gggggtcgga tttgattttt cgagtatagt ttgcaggaca ggcggcaggt gactatcgat
     1561 gaactcggtg ttggcacgat agccatcaat cgggctgtta ttgtgctggt aggagccatc
     1621 tggagcaaat ataaacggcg agtggggtac catgatatgg acaaagacga attttggccc
     1681 aggagaatcg ggaactttct ccagttcggc gagaatgtaa ttcgtctggt aatagtgctc
     1741 ccacaactcc cattcctgtg tgcgggtgtt gtcgaagcca gggatcagtt ctgtgtcaac
     1801 cacaaagcgt aaaaatgacg tgtcaatcag cattttctca tattcactga tgccaccgat
     1861 aaagtcaaat ttgccaaagg ccatctggtt gcgcgaaagc aggacatcct ccttgaggtc
     1921 gaaatggccg ctggcgcgat tttcaaaggc gaagatttta tagcccaaag atttgagcgt
     1981 ctggttgagg gcacttcttt tcaaagaggg taagatttcc ccttcctcga cgaagttctg
     2041 gatgtaatcg ccgtaaaagg tggaggtcag cgagagttta gtggatgcgt aattgctctg
     2101 gctacaactg gccacgtaaa accccatatc attcagcttt tgtataaagc ttgagttgtc
     2161 aaatccaaag cgcgcttcaa gcacgtccgt gcgtgtgtga gcatccaaga taatcaggta
     2221 taagtctggc agtttctcgt cagcctgtac aggcgcaacg ggagcttctt cttgggcaga
     2281 cgcggcttcg gcccgataca cgccaatgtc gtagttaaca gtttggaata ggactattgc
     2341 gaccaggaca atactggttg tggcaaggaa ttgcgccagc accctggcga cctggtcttt
     2401 cttcaagatc aggatggtaa ccagtagcaa gatcaagaac gatgcggcga caagatagcg
     2461 atgccggacc gggctgccca aggtctcttc gatcttgata tagatgtgac cgtaggacag
     2521 gaagaacatc ccgattaaag acacgatgat gctggtttta gccaaattgc ggacgaacag
     2581 gtgggtaatg gcaatgagga tcgctgtacc agctgtcacc aggaggaggc tgcgcaggat
     2641 agacggcagg ttgacgtaga caatgttgtg ggcgcgcaga gccagaatcg ggtaatagac
     2701 cagtaaaaac gggtaaatta gcaggcttgc tctgtgccag agggatggct tgctggcggc
     2761 ttgatcattg gacataaaaa aagccttcca ttgtcaggca gtcaagaggc ttggacttta
     2821 ataccgccag agtatatcat aatggacggg cgaaaaagtc gccagcccga tgcagggcta
     2881 aacatcccca cctaattttg agcagcctat cagtacgctg tgctagttct ggacgaaggt
     2941 gccgttgcgc agatcgttca acgcttgctg gatctcttcc tgggtgttca tcacaaatgg
     3001 accatatggg aagatcggct ctttgaacgg cgcgccagcc atcagcatga agcgggcatg
     3061 agagccttgt ccagcttgga cgcgaacgtg gtcgccatct tcaaattcga tcatgcgcac
     3121 cgcgggggta aattcaccct gcccttcagc gtccagaccg aataacccct ccccctcgaa
     3181 aagataggcg atggtggtat ggcctactgg gacaggcaag tagaattccg cgccaggatc
     3241 gagtgtcacg tccatataga gcggttgggc ggcaatctgt gtcacaggtc ctttaacacc
     3301 cccgaattca cccgccacca ggcgtacttt tgcaccctgt ttttctgcgg ttgggatact
     3361 gtcggcattc acttcctgat agcgcggctg gctcatcttc tgggcggcgg gcaggttgac
     3421 ccatagctgg aagccataga tgacgccatt cggcccgcgc ctgggcatct cttcgtgtaa
     3481 gattccgcgc ccgctggtca tccactgtac atcgccggga ccaatgatgc cggtattgcc
     3541 caggctgtcg cggtgtcgca cattaccttc cagcatgtat gtaacggttt caatgccgcg
     3601 gtgtgggtgg gtgggaaagc cacggatagg gccttcgatc ggatcgttaa aagcaaaatg
     3661 gtcaaacaga agaaacgggt cgaaaaggtt ggtgcggttg ggtccaaagg agcgccgtaa
     3721 cagcacgcct gcgccttcga taacgtactg cggctcgata acctgtttaa ctttgcgagg
     3781 ggaatttgac ataaaaccaa ctttctatct aaaaaggata atgataatca tgattatacc
     3841 aaatcccgtt gccagacccc tgcaagtata tgaatttata taaacttctt agaaaaccaa
     3901 tatcttgaat aataagctta aattgtatat actataactg actgaccagt cggtttatta
     3961 tagagagaag catatgtccc ctcgccctga cgtaagccta gaacgcaaac accagatact
     4021 gaacgccgct gaagaagtct tcagccgtaa gggttttgat gaagctcgca ttgatgatat
     4081 cgctgaagag accggcttga gcaaaggcac aatttactgg tacttcgaca gcaaagacga
     4141 catcattatt ggtatcttgg atcgcatttt tcagcgcgaa ttcaagcaca tggaaagtct
     4201 taaccttctt gagttgccgg ccaatgaggc ggtttggcaa tatgtcaaga ttgcctccca
     4261 ggacatcacc aatatgttgc agctgatgcc aatcgcctat gaattcctgg cgctagcatt
     4321 tcgaaatcag atcgttcaaa aggcgctgca gcaatatttc ggccgctaca tggacatcct
     4381 tattcccatt atccagggcg gcattgactc gggcgagttt cgcacggtgg aggcgcgcga
     4441 ggtcgccata gcgattgggg cgatcttcga agggacgatt ctattatggg tatacgatca
     4501 aaatatggtg gagcctgaac gccatatcag ctccgggatt aggctgttgc tcgaggggat
     4561 aagggcttag ccctcgctac ccatgcccca acagcgtagc ttctacaaat aaaggagttt
     4621 gagacatgct gcaagtaatt atcttagtcc tggcaatggt ccttgtcggc ctgttggttg
     4681 gctggctggc tggcctaatc tggaaaaatc agcgtccgat cggtgtaagc ggggattaca
     4741 tggtggctat catctcggct attgttatcg gattgattga ctggtttgtg atcccagcta
     4801 tgggcttcag cgatacgatg aaatatttgg gcgtggcttt cgaacctttg ctgggcgcgc
     4861 tgctcgtgct ttggatcatc cgcaaagcca agcagtaaat ggagatgatc gtggttccgg
     4921 ttatcgagac ccacgaactc gcacgaaact ttaaagccac ccgcgcggtg gatgggttgg
     4981 atttatccgt acagccaggg gaactttttg gattggtagg gccggacggg gcggggaaga
     5041 ccactacctt acgcctgctg gcggggctgc tggacgtgag cggcggcgag gccaccatcc
     5101 ttggttttga tctcaagagc cagccggagg ccatcaaacc acgggttggt tatatggcgc
     5161 agcagttcag cctgtacgcc gaactttccg tgctggaaaa catgcgcttc ttcgctgaac
     5221 tttttgatgt ctcagcccaa gacttgtccg aacggatgca agggctgctg gtttttgccg
     5281 gcttgaccga attccaggac cggcgcgcgg cccatctttc gggcgggatg cagaagaaac
     5341 tagccttggc gtgtaccttg gttcaccggc cggaagtcct gctgctcgac gaacccacca
     5401 cgggcgttga ccccatatcg cggcgcgagt tctggaatat tttgactgaa ttgcaccttg
     5461 atgggacgac cataatggtc agcacgccct atatggacga agcggagcgc tgctcgcggg
     5521 tggggttgat gttcactggc aagcttgtag tgtgtgatac gccccaaaat atccgcaatc
     5581 ggctggaagg agacgttatc gagttgtaca gcgaggattg gcaatctgcg cgagaaagag
     5641 tcgcagggtt gccgggcgtc cgtgaggtgc agacttacgg cgagtcgttg catctggtgg
     5701 tcgatgcggg agaaaaacgc ctgcccgaaa tcgaaagcgc gttgaagaag gccggcgttg
     5761 acttgcggag catgcgcatc gctccacctc gcatggaaga ggcgtttatt tcgctgatcc
     5821 gcaaaatgga gaaatgatgt cgatttctca catctgggcg gttacccgca aggaatttct
     5881 ccacatcatc cgcgaccgga gcacgctctt cctggtgctt tttacaccca ccctggtgct
     5941 cctgctgatg gcctatgccc tgactgtaga tattaagcac gtgccgattg gcatactgga
     6001 ctacgatcgc agcccaactt cgcgcgcttt tatccagcag ataaccgccg ggcaggattt
     6061 ggatatttat gcttatgcag aaaacatgga tgagatcgat tccatgttga tgcacggaaa
     6121 aattaaagct gccctggtca tcgctccggg attttccagt gaacttcagg ggcttaaagg
     6181 gatgccgata caggtcatta ttgatgggac agagccggaa agcggcaact ttgccatgca
     6241 gcacatcacc cggcgggcgg aagaatttat tgaccaggta ttgacacgcc gtttgcaggc
     6301 cgcgggcgtc tcgctcgcct cgttgcaacc actagacttg cgcgtgcata cctggtacaa
     6361 ccccgatctt aaaccccgca atgacctgat ccctggcctg atctcaatgg tgttgggctt
     6421 cccagcccta tctgtggcgc tgacgctagc tcacgaacgc gagcacggga cgctggagca
     6481 actcctggcc acgcctatca cgcgcggcga actgctgctg gggaagatcg tcccgtatct
     6541 cgcggtaggt ctggtgaatg tagtgatgat ccccgcgctg gcaatcgcct ggttccaaat
     6601 ctccttcaat ggaaactttc tgctatattt tggcctctcg gcgatcttcc tgtttgctgt
     6661 gctgagcatg ggcatgattg tgggcgtgtt tatgcgtact caggctgctg cgcttgcact
     6721 ttcgttcctg gtgatattct ttcctggctt cttcctgacc ggtattttct tcccattggc
     6781 ttcaatgcct gaagaagtgc gcatggaagc catgatgctg ccgggcacgc actacgcggt
     6841 gatcacgcgc ggggtcttcc tgccaggcgt gggtctggag gtgttgtggc cgtatgccgt
     6901 gatgctcatc gtgctgggtc ttgcatttac gggactggca gccttgtttt tccgtaagaa
     6961 attgggatag ctcatgctga ggcgaatctg gatactggtg atcaaagagt tcatccactt
     7021 gcgcacagac tggtggctac cagccttcat gctgctgggt ggggcgatgg agcttttgct
     7081 ggttggctgg gcgacctcgc gtccgatcac caatctgcct ttgatggtgc tcgaccagtg
     7141 caatagccct gccagtcgat ctcttttggt agctctagag aatacgggca cgtttcagct
     7201 acaggagcag gttagcgaca tggtcacgat tgaatcggca ctggagcagg gcaagatcaa
     7261 tgcggctttg gttatcccac cagatttcag cgagcagatc aactcgtctt atgggcgccc
     7321 gacactgctt gcgatcctaa acggtgcaga aagcacgccg gcgatggccg cgctgcgcgc
     7381 catcgatgga gtcagccggg aagtagataa acgcatactc atccagcgac taggcttgag
     7441 cgcggatgaa ttttcggatt tcaatcttag cctgcgggtg tggttcaatg aagagctcag
     7501 cgaagcttta tacactactc cggctgagct gggcctgatg ctagagttta cagtgctgct
     7561 ctttgcagcc ttggcctttt cacgcgaacg cgaattgggg acacttgaac aactgctggt
     7621 aatgccattc tcctcgctgg agatcatcat cggcaaagcc atcccagtga tcatcgtggg
     7681 ctttaccgat tttctgctca tgcttggcat ggtacatttc gcttttggcg tgccagtgcg
     7741 cggatcgttg ccactgctcc tggttttggc tctgggctac ctgttagttg agttgagcaa
     7801 gggcatggtc atttcggtta tctcacgcac ccagcatcaa gcctttttgc tggtgatgct
     7861 gctgggtatg gtggatttca tgttcaccgg ctatgctgct cccgtggatg gtatgcccaa
     7921 agccttgcaa tactttgcta acctgattcc cgctcaccat tggttgagca ttctgcgcgg
     7981 catactgctc aaaggcgccg ggctggatgt gctctggcag catgttttga ccctgttcat
     8041 cttgggattg ctgattggta ctttctcttg gcgcttcgtc cgccgggcat tggattaaat
     8101 cgatgttgga aagaacacct gccatcgtgg cgcacaaatt aaccaaacgc ttcggcgact
     8161 tcaccgctgt gaacgatgtg gactttgaag tacagcgcgg ggagattttt ggcttcttgg
     8221 gtcccaatgg atcgggcaaa actaccacaa tccgcatgtt gctggggctg atgaagccaa
     8281 gcgccgggtc ggtggaagtg ctcaatatgc ctgtggatgg agacactcaa aagattcgcc
     8341 cgcgtgtggg gtacatgtcg cagcgtttca gtttgtataa tgacctgagc gtgctgcaaa
     8401 acctgcgctt ttacggagct gcatacggat tgtctgacgc tgaattggaa gtgcgcattg
     8461 gtgaagccct ggtgatgtgt gggctggaag ggcgcgaaga ttccaaaacc aaagacctat
     8521 ctggagggtg gcgacagcgc ctggctctta gcgcggctat cttgcaccgt cctgaagtgc
     8581 ttttcttgga cgagcctaca gcgggcgtgg acccggtctc gcgtcgcgtt ttctgggatt
     8641 tgctgtatgc cctggttgtt cagggggtga cggtctttgt caccacacac tatatggatg
     8701 aagcagaaca ttgcca
//
LOCUS       DBNGSX010000084         4589 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1391605, whole genome shotgun sequence.
ACCESSION   DBNGSX010000084 DBNGSX010000000
VERSION     DBNGSX010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1391605"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..837)
                     /locus_tag="AC16CB_02815"
     CDS             complement(82..837)
                     /locus_tag="AC16CB_02815"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016972.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3341429.1"
                     /translation="MSTQNPIQLIVFDIDGVLTEGETKPLDLELLADLARLNRESRHN
                     PIFPAVTLCSGRPAQYVELMLQAIDGYLPGVFENGAGLYVPETYRFLPNPALSNNGKM
                     IHIIRQRLEETIIKSGEAYIQPGKEYSLTLFSSDPGRTSKLYDLVVSVLGDLMREVKL
                     VYSTSCLNILPRQIDKGEGIRFLSEWTGYALSGMLGVGDSDVDQEFLSVVGYSAAPAN
                     SHPEIKAIVQYVSPLRTGQGVRDILAHFNIPLP"
     gene            1203..2195
                     /locus_tag="AC16CB_02820"
     CDS             1203..2195
                     /locus_tag="AC16CB_02820"
                     /inference="COORDINATES: protein motif:HMM:NF014110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341430.1"
                     /translation="MDNHIILATYDRGISRAERGSDGNWAVEQHATDYEVTCLAADPS
                     NPQTIFAGTRNGMLRSDDQGLTWYLSGMEGQIVKSLAVSPHDSNIIYAGTKPALMYIS
                     RDGGENWSELEGFRRIPNRWWWFSPAEPPDRRPYIISIAVSPSDPNVILAGIEFGAVV
                     RSADGGQSWSRHRTGALRDCHSLKFHATDGNWVYQAGGTGGGASLSQDGGLTFRKAKR
                     GLAKHYGIVCAADPERPDIWYVCVAPSPFNAFGEHPEIYLYRAKGDAGWEPIGWQPHP
                     LPVTPTALTTVHNKPAHLYAGLHNGDVWLTTDYGDSWTKMPFSFKGIWFSLLVI"
     gene            2418..4439
                     /locus_tag="AC16CB_02825"
     CDS             2418..4439
                     /locus_tag="AC16CB_02825"
                     /inference="COORDINATES: protein motif:HMM:NF016648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HIR3341431.1"
                     /translation="MMETNGKRNFPCLVILLAAGLVIVLACGVAATLGIGTFFWLTQR
                     TVTTSQSEPLQASSKINPQDAILTPTRGLVEIQDQAGGWSVLAAEMPISEGSRVRTGA
                     LSGAQLIFYNGSRATLWPNTEIAIEKLDFQDGRQVVVLTQSTGESDHQVVSSPENKTR
                     YEVHTPAGSAEAKGTSFRVSVTPDHSAHFTVEEGSLAVSGQGESVVLEAGQTSAIYTD
                     QPPTEPALRVSGEGVVTQTGATWVIDGQSYTVHEHTIIIGNPRVGDIVHVEGRLLADD
                     TRLADLIVLLRTSPANRFTLIGVVEEIDEQAWTVAGQTIEIGENADIDPSIQEDDLVR
                     VEGLILENGALQAEKILPAGEEDDLRFEFTGVVETTGTSSWKISGIEVVIDEDTTIDD
                     ELKSGDLVRVRGIIQENGDWLARRITRAIEGDRFFEISGHIESIDPWKVAGVSFETRS
                     WTDIEPGLELGDLVRVKGQVQEDGTWVAHQIERIQDIPSPLIVIIGKVISTDPWVVSG
                     IPLNVTSETIIEGDIVPGMLVRVEISLLPDGTWQVLRITPLDEFTGIPGCTNLTATVV
                     SFDGNRLQLLGWPILVLGDEVKIDGKLRHNAVVVVQICFNEDGTFEVIQIIVIFLPEI
                     DVEPPPVEGHKVTICHKPYKKKGGNTINISRSALPAHLGHGDYVGACSR"
BASE COUNT         1072 a         1276 c         1263 g          978 t
ORIGIN      
        1 cgggtaacat cctcataccc aggcggtcgg ctccaactac tggctctcag cattggttcg
       61 actgccttgt tgatcatcgc ctcaaggcag agggatatta aaatgcgcca ggatatcgcg
      121 cacgccctgc ccggtccgca agggggatac atattggaca atagccttga tttcaggatg
      181 gctattagcg ggcgcggcgc tgtagccaac gactgacaga aattcttgat ccacgtcaga
      241 gtcgcctacc cctagcatcc ccgacaatgc gtagcccgtc cactcagata aaaaccggat
      301 cccctccccc ttatcgatct ggcgcgggag gatgttcaaa caagaggttg agtaaaccag
      361 cttgacctcg cgcattagat cgcccagcac agaaacaacg agatcataaa gcttactcgt
      421 acgccctgga tcgctggaga agagggtcag cgaatattcc ttgccgggtt ggatgtaggc
      481 ttcgccagac tttatgatgg tctcttccag gcgctggcgg atgatgtgga tcattttgcc
      541 gttgttggag agggccgggt tgggcaggaa gcgatacgtt tcaggtacat agagtccggc
      601 accgttttca aatacacccg gcaggtaacc gtcgatagct tgcagcatca actccacgta
      661 ttgggccggg cgccccgagc agagtgtcac ggcggggaaa atcgggttgt gacgggattc
      721 ccggttcagc cgggccaggt cagccaacaa ttctaagtca agcggcttgg tttccccctc
      781 ggtcagcacg ccatcgatat cgaagactat cagttggata gggttttggg tagacatagg
      841 aaactgatta tatagccagc gctgcgacct gccaaggttc gagatgtcga acctaaccta
      901 ctaagaaaat gtaggggttc ccccttgatg gcgactaact taaactccac aacagacttt
      961 ttcgccgcgg attgaaatct gccgcttagg ataggcacat ttctttgccc cagagaattg
     1021 ctactccatt gagaaggagc actcctttcc caatcaaata tcggctccca ggatgccgta
     1081 ctggtgctca cttgtggctt ggcggaaatc aaagacctgt caggcatacg tgtataatct
     1141 agttgggtta taaatttagt gctcagctca cgggcatagt atcccatagg gaataacaac
     1201 cgatggacaa tcatatcatc ctggctacat acgatagagg tatcagccgc gctgaacgcg
     1261 gctctgatgg caactgggct gtcgagcagc acgccacaga ctatgaagtc acctgcctgg
     1321 cagccgatcc gtcgaatcct caaaccatat ttgcgggtac gagaaacggc atgttgcgat
     1381 ccgatgacca gggtttgacc tggtatctca gcggcatgga agggcaaatc gtcaagtcgt
     1441 tggccgtcag cccccacgat agcaacatca tctatgctgg aacaaaaccg gccctgatgt
     1501 atatctctcg cgacggcggg gaaaactggt cagaattgga aggcttcagg cgcattccca
     1561 accgctggtg gtggttctcg cccgcagaac cacctgatcg ccgcccatac attatttcta
     1621 tagcagtttc tccctcagac ccaaacgtga ttcttgccgg tatcgagttc ggcgcggtag
     1681 tgcgcagcgc agacggcggg caatcctggt ctcgccaccg cacaggcgcc ttgcgcgact
     1741 gccattcgct caagtttcac gccacggacg gcaattgggt ctaccaggcg ggcggcaccg
     1801 gtggtggggc ttcattgagc caagacggcg gcctcacctt ccgcaaagcg aagcgcggtc
     1861 tggctaaaca ttatggcatc gtctgcgctg ccgacccaga aagaccagac atctggtacg
     1921 tttgcgttgc acccagccca tttaacgcct ttggcgagca cccagaaatc tatctttacc
     1981 gggcaaaggg cgatgctggt tgggagccta tcggatggca accccatcca cttcccgtca
     2041 caccgacggc tctcaccacg gttcacaaca agccggctca cctgtacgcc gggctgcata
     2101 acggcgacgt ctggctgacg acagattatg gtgactcctg gacaaagatg ccgttcagct
     2161 ttaagggcat atggttttcg ttgctagtta tctaatcttt cgataaacat atccggaatt
     2221 ctgtataggc aggttgtatc tacctgatca ctgaacaata cgaacacagt ttcgcatagc
     2281 tcgactccca aatacaggct ataaaaatct caccgccgaa actgatctcg aatttacgct
     2341 ttcgggctta atcattcctt atctctaccc acacccatat acgatataat ccatataatg
     2401 atctgtcgta ggagggaatg atggaaacaa atggcaaacg taatttccct tgcttggtga
     2461 tcctgcttgc tgcagggctt gtgatcgttt tagcctgcgg agtcgctgcc acgctcggga
     2521 taggtacatt cttttggctt acgcaacgaa ctgtcacaac cagccagtca gagccgctcc
     2581 aggcttcatc aaaaataaac ccccaggatg ccatactgac ccccacccgc ggcctggttg
     2641 aaatccaaga ccaagcaggt ggctggtctg ttcttgccgc ggagatgccg atctcggagg
     2701 ggagtcgcgt gcgcaccggg gcgctatccg gcgctcagct gatattttac aatggcagcc
     2761 gggcgacact ttggcccaac accgagatag cgatcgaaaa actggatttc caggatgggc
     2821 gccaggtggt cgtgctgacc cagagcacag gcgaaagcga tcaccaggtg gtgtcatctc
     2881 ccgagaataa gacgcgctac gaggtgcata ccccggcagg cagcgccgaa gccaaaggca
     2941 cgtctttccg cgtcagcgtc acgcctgacc attcagcgca tttcactgtc gaggaaggct
     3001 ccctggcggt aagcgggcag ggcgaatccg tcgtcctgga ggccgggcag acctccgcca
     3061 tctacaccga ccagccgccc accgaacccg ccttgcgtgt ttccggcgag ggggtggtca
     3121 cccaaaccgg agccacctgg gtgatcgacg ggcagagcta caccgtccac gaacacacca
     3181 tcatcatcgg caacccgcgg gtcggggata tcgtgcacgt cgaggggcgg ctgctggcag
     3241 acgatacgcg cctggccgac ctgatcgttt tgctgcgcac ctcccctgcc aaccgcttca
     3301 ccctgattgg tgtggtggag gagatcgacg agcaagcctg gacagtcgcc ggacagacga
     3361 tagaaattgg agagaacgca gatatcgatc cttcgatcca ggaggatgat ctcgtcaggg
     3421 tggagggcct gatcctggaa aatggcgccc tgcaggccga gaaaatcctg cctgctggag
     3481 aggaggatga tctgcggttt gaatttaccg gcgtggttga gactaccgga acgagcagct
     3541 ggaagatttc agggattgag gtcgtcattg atgaggacac aaccatcgac gatgaactga
     3601 agtccggcga cctcgtccgg gtgcgcggca ttattcaaga aaatggagac tggctcgcca
     3661 ggcgtatcac gcgagccatc gaaggcgatc gcttcttcga gatctccggg cacattgaga
     3721 gtatcgatcc ctggaaagta gcgggggttt cattcgagac ccggtcttgg acagacatcg
     3781 aacctggact ggagctaggc gacctggtgc gggtgaaagg ccaggtccag gaggatggca
     3841 cctgggtggc ccatcagatc gagcgcatcc aagacattcc atccccgctc atcgtaatca
     3901 ttggcaaggt catcagcacc gatccctggg ttgtcagcgg catcccgttg aacgtcactt
     3961 cggagacgat catcgaaggg gatatcgtgc ccggtatgct ggtgcgggtc gaaatctccc
     4021 tgctgccaga tgggacatgg caggtgcttc ggatcacgcc gctggatgaa ttcaccggca
     4081 tacccggatg cacgaacctg accgctacgg tagtcagctt cgacggaaat cgcctccagc
     4141 tcctcggttg gccgattctt gtgctgggtg atgaagtaaa aatcgatggc aagctgagac
     4201 acaacgctgt cgtcgtggtc cagatctgct tcaatgaaga tggcactttt gaggtcatcc
     4261 agatcatcgt catattcctg ccggagatcg atgtcgagcc gccgccggtg gaggggcata
     4321 aagtgacgat ctgccacaaa ccgtataaga agaaaggcgg caacaccatt aacatctcgc
     4381 gctctgccct gcccgcccat ctgggacatg gggactatgt cggggcttgc tcaagatgac
     4441 gtgaatatcc cctgattttc acaagggatg ggacaacagc gtgaccaaag cttcggtcac
     4501 gctgttgctt ttataaactg agccaatcac caattaggtc agcataagat tcatgacccc
     4561 ttcgtatgac ctccggcact tccaaacat
//
LOCUS       DBNGSX010000085         3486 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_421986, whole genome shotgun sequence.
ACCESSION   DBNGSX010000085 DBNGSX010000000
VERSION     DBNGSX010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_421986"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="AC16CB_02830"
     CDS             complement(<1..326)
                     /locus_tag="AC16CB_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341432.1"
                     /translation="MSQEEQVLLFNQQLDRLLADEMPDKVLPDLDEQTLRLAQHLSQV
                     NFSDESLNRHALYTRLRKLSSTGNSKTARHWLPSIIRITSFQVQFVTWVRATLVLLSV
                     LFFGLG"
     gene            complement(323..895)
                     /locus_tag="AC16CB_02835"
     CDS             complement(323..895)
                     /locus_tag="AC16CB_02835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3341433.1"
                     /translation="MTTFETKIIRKAAIAEQSLNDRHAFLDRYHEYFPRVYNYVRYRC
                     GDQSITDDITSIVFEKALTKFHQYSPKRGSFGAWLFAIARNEVNAYLRSLLGRNRIVP
                     MEQVQDWPTGKLSPEEALIDGELRNELIAALEGLNERERDLLGLKFSARFTNRQIASM
                     TGLTENNVGIILYRALQKLRRQLIPLEAQK"
     gene            complement(1028..2911)
                     /locus_tag="AC16CB_02840"
     CDS             complement(1028..2911)
                     /locus_tag="AC16CB_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CASTOR/POLLUX-related putative ion channel"
                     /protein_id="HIR3341434.1"
                     /translation="MSKHNLRRRLNYWFDNLMARGTIMHIAWLFIFSAMMIFSIAALA
                     RFTEDGQQVSLMQLAWNSLMRTLDPGTMGGDEGSWPYLFLMFTITLGGIFVVGTLIGI
                     ITNGIDEKLTALRKGRSIVIESGHTVILGWSNQIFSIISELVVANQNIPRACIAILAN
                     KSKVDMEDELRDRIGPTGNTRLVCRTGDPIELANLEIINPSTARSVIILPPDSDIPDA
                     QVIKLVLALSHHFNKSNFHIVAEFQNSKNLEVARLVGGDRGFFIQANELIARITAQTC
                     RQSGLSVVYNEILDFEGDEIYFQLEASLFGKTFGMALLAYEDSCVIGLRVRDGIIRLN
                     PPMDTIIEPGDAIIAISADDDTVRVSGLTNAPVNENAIQNPVPSLKGPEHTLILGWNH
                     LAPMIVKELDNYVAPDSGVLIVADVQNSIMESSFELENIKNQKVSFIQGDSTDRQTLD
                     KLDIPNFQHVIVLSYSDQLDTQQADARTLMTLLHLRDIGNQSKNRFSIVSEMLDFRNR
                     ELAEIARPDDFIVSDRLISLMLSQISENKELIHVFDDLFNPQGSEIYLKPAGDYVHLG
                     TPTNFYTVVESARRRDQVAIGYRLNDLSHDPRMTYGVMVNPDKSELVTFSEQDRIIVL
                     SEE"
     gene            complement(3044..>3486)
                     /locus_tag="AC16CB_02845"
     CDS             complement(3044..>3486)
                     /locus_tag="AC16CB_02845"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR3341435.1"
                     /translation="PVGRLDVDSEGLVLLTNDGELANKITHPRYGHEKEYQVLIARRP
                     DNDQLQTWRRGVVLEDGHRTAPAEVNFVRTHGKGAWLRIVMREGRKRQIREVGGRLGL
                     PVVRIIRTRIGTLLLGNLKPREWRHLTQREINDIKNSIKSSSSS"
BASE COUNT          897 a          800 c          847 g          942 t
ORIGIN      
        1 attcccaggc caaaaaacag tactgacagc aataccaaag tggctctaac ccaggtaaca
       61 aattgcacct ggaaagaagt aattctaatt atcgaaggga gccaatgtct cgcagtttta
      121 gaattgccag tagaagatag ctttctcaaa cgagtgtata aagcgtgtct gttgagactt
      181 tcatcgctaa aatttacttg ggataagtgt tgggcaagcc ttaatgtttg ctcatcgaga
      241 tcaggtaaga ctttgtcagg catctcgtct gccaggagtc ggtctaattg ttggttgaag
      301 aggagcacct gttcttcctg gctcattttt gcgcttccag agggataagc tgacgccgaa
      361 gtttttgtag ggcgcgatac agaataatac ccacattatt ctcagtgagg ccagtcatcg
      421 aagcgatctg gcggtttgta aaacgggcac tgaattttag gcctaataga tctctttctc
      481 gctcgtttaa gccctccaat gcagcgatca attcgttgcg caattcgcca tcaatcaacg
      541 cttcctcagg ggatagcttg ccagtaggcc agtcttgcac ttgttccatc ggcacaattc
      601 ggttccgccc aagcaaagat cgaaggtacg cgttgacctc atttcgggca atcgcaaaca
      661 accaggctcc aaaggaacca cgctttgggg agtattggtg aaatttcgtc agcgcttttt
      721 caaacacgat cgaggttata tcgtccgtga tgctttgatc gccacaacgg tatcgaacat
      781 agttatagac tcgaggaaaa tattcatgat aacggtctaa aaaggcgtgc ctatcgttga
      841 gcgattgctc tgcaatggct gccttgcgaa taatcttggt ctcgaaggtg gtcacaacaa
      901 tgccatgtag tcgaatagaa gttctactag ttaatatgtg ctttggcgaa aagtattaca
      961 taatgtcgcc ttgttctgga aattatcaca caacgagaat caccgattat attttttgca
     1021 ggattgttta ctcctcagag agcacaatga tacggtcttg ctctgaaaaa gtaaccaatt
     1081 ccgacttatc gggattcacc atcacgccat atgtcattcg agggtcgtgg ctcagatcat
     1141 tcaggcggta tccgatagcg acttgatccc gtcgacgcgc cgattctacc actgtataaa
     1201 aattggtcgg cgtacccagg tgaacgtagt ctcctgcggg cttaaggtaa atctctgagc
     1261 cctgtgggtt gaataaatcg tcgaagacat ggatgagctc tttgttctcg gagatttgtg
     1321 agagcatcaa gctaatcaat cgatcgctga caataaagtc gtcagggcgg gcaatttcag
     1381 ctaattcacg atttcgaaaa tctaacatct cgctgacaat tgagaagcga tttttggatt
     1441 ggttaccgat atcacgcagg tgtagaagtg tcatcagggt gcgggcatca gcctgctggg
     1501 tatccaattg atctgagtag ctcaatacga tcacgtgctg gaaattaggt atgtcgagct
     1561 tgtcgagtgt ctggcgatcg gtggaatcgc cctggatgaa cgaaactttt tggttcttta
     1621 tgttttcaag ctcaaaagag ctttccatga tgctattttg cacatctgcc acaattaaca
     1681 cgccagaatc tggggcgaca taattatcca attctttgac gatcattgga gccagatggt
     1741 tccaacccaa aataagcgtg tgttctggcc cttttaggga tggtacagga ttctggatgg
     1801 cattttcatt tacaggggca tttgtcagtc ccgagacgcg gaccgtgtca tcatccgcag
     1861 agatggcgat aattgcatca cctggctcta ttatcgtgtc cataggaggg ttcaggcgga
     1921 tgattccgtc tcgaacgcgc aagccgatca cacaagaatc ttcgtaagcc aacaaggcca
     1981 tcccaaatgt cttcccaaat aggctagcct ccagttgaaa gtaaatctca tcaccttcaa
     2041 agtctaagat ctcgttatag acaactgaaa ggccagactg gcggcatgtt tgagcggtaa
     2101 tacgggctat cagctcgttt gcctggatga aaaaacctcg gtcgccgccc accaaacgtg
     2161 caacctcaag atttttagag ttttgaaact cggctacgat atggaaatta cttttgttaa
     2221 aatggtggct gagagctaag acgagcttga tcacctgtgc gtcaggtatg tcgctatccg
     2281 gagggagaat gatcaccgat ctagcagttg aggggttgat gatctctagg ttggctagct
     2341 cgataggatc acctgtgcgg cagaccaagc gcgtgttgcc agtggggcct atacggtcgc
     2401 gcagctcatc ctccatatcg accttacttt tatttgccag gatagcgata caagcacgtg
     2461 ggatattttg gttggcgacc acaagctctg agattattga gaaaatttga ttagaccatc
     2521 caagtattac cgtgtggcct gattcgatca cgatggagcg gcctttgcga agagcggtca
     2581 atttctcatc aataccattc gtgatgatcc caatcagtgt tccaacgacg aatattcctc
     2641 ctagtgtgat ggtgaacatg aggaaaaggt agggccaact tccctcgtca ccacccattg
     2701 tgccggggtc aagtgtgcgc ataagactat tccacgccaa ctgcatgagc gatacttgct
     2761 gcccatcttc ggtaaaacga gccaacgcag cgatcgagaa aatcatcatt gctgaaaaga
     2821 tgaacagcca ggcaatatgc atgatggtgc ctctggccat caggttgtcg aaccagtagt
     2881 ttaatcgtcg gcggagatta tgtttgctca tggtccaact gacatcttga tgatagaaat
     2941 taattctgac ctaaataata ttcggttgat gagtatggcg tgacgaagcc gttgcaatag
     3001 acgcaatcat cttaacttag acgtttttaa gtaatacgtt ggattatgat gatgacgaag
     3061 atttaatgct gttcttaatg tcgtttattt cccgctgggt gagatggcgc cattctcggg
     3121 gtttaaggtt tcccagtagt agtgtcccaa tgcgagtgcg gatgatgcgc acaacaggga
     3181 ggccaaggcg cccccctacc tcgcgaattt gccgtttgcg cccctcacgc attacgatgc
     3241 gcagccaggc tccttttcca tgtgttcgta caaaattcac ttcggcaggc gctgtccgat
     3301 gaccatcttc tagaaccacg ccacgccgcc aggtttggag ttggtcatta tcagggcgtc
     3361 ttgcgatgag cacctgatat tccttttcgt gcccgtagcg tgggtgagtg atcttgttgg
     3421 caagctcccc atcgttggtg agaagtacca acccctcact gtccacatcc aatctaccaa
     3481 ctggat
//
LOCUS       DBNGSX010000086        16610 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1530377, whole genome shotgun sequence.
ACCESSION   DBNGSX010000086 DBNGSX010000000
VERSION     DBNGSX010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 16610)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16610)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16610
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1530377"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="AC16CB_02850"
     CDS             complement(<1..418)
                     /locus_tag="AC16CB_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR3341436.1"
                     /translation="MSKLVTLTIDGLQVSVPAGTLLVNAAKKIGIDIPVFCYHPKMEP
                     VGMCRMCLVDIGRPVIDRATGQPVLEADGSPKIQFGPKLETACTTPVSEGMVVVGMSD
                     KVKAGREDILEFLLTSHPLDCPICDKGGECPLQNLTM"
     gene            complement(485..1777)
                     /gene="nuoF"
                     /locus_tag="AC16CB_02855"
     CDS             complement(485..1777)
                     /gene="nuoF"
                     /locus_tag="AC16CB_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HIR3341437.1"
                     /translation="MNKYLLLRNRELPTINRLEVYRQHGGFQAFHKVVTSMQPDEVIE
                     VVKASGLRGRGGAGFPTGLKWAFVDKKSWPHYVVANADESEPGTFKDREIMEGNPYQF
                     LEGVAIAAYAVQAKAAYVYLRGEFWQIASQLDQNIAALELAGLLGDNLFGTGYNLHIY
                     THLGAGAYICGEETALLESLEGKLGQPRLRPPFPPSYGLYGKPTVVNNVETLANVPLI
                     LDRGAEWYRTYGTEKSPGVKIFSLSGKVNRPGNYELPLGTTFRELIFEHGGGIPNRNK
                     IKAIMPAGASSSLIVATDQALDTPMDYESVPSVGAQLGSASIIVIDDSVNIPWLINKT
                     IHFFKHESCGKCTPCREGTYWMMHLTDRIADGKATQNDVELLHDVASQIKGKCLCALG
                     EFSIEAVLSGIDRFKDDFDLQTGGQQSVVQSEESAQTP"
     gene            complement(1774..2274)
                     /locus_tag="AC16CB_02860"
     CDS             complement(1774..2274)
                     /locus_tag="AC16CB_02860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013426.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE family
                     protein"
                     /protein_id="HIR3341438.1"
                     /translation="MLTEKYPEEIRTILAKYPSDQKRSAVMPLLYLAQREQGYVTKQS
                     MSEIAEILGISSTDVASIVGFYTLYYDEVGGKYHLQVCTDLPCALRGADEFLENLCQN
                     LGIRVGETTPDGLVTVEAVMCLAGCDKAPMFQVQSGDGLTYHENQTVESAMNLVKSWR
                     KEEGRA"
     gene            complement(2289..3518)
                     /gene="nuoD"
                     /locus_tag="AC16CB_02865"
     CDS             complement(2289..3518)
                     /gene="nuoD"
                     /locus_tag="AC16CB_02865"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HIR3341439.1"
                     /translation="MLEKTEVSLDELRHLVSARAIEGETVLLNMGPQHPSTHGVLRLL
                     LELDGEIVVNCIPDVGFLHTGIEKNMEAKTYEKAAVMTDRLDYLNTTGNNLVYCLAIE
                     KLVDLDVPLRAQAIRVILTELQRIASHLVWLGTHALDLAAMSVFLYCFREREMILDVL
                     EMCGGQRMMSTYFRPGGLWRDIPVEFEEAVRQILRVFPARIDEYEALLTKNPLFIERT
                     KKIGVLSRQDAIGWGITGPSLRASGVAHDLRKAQPYSGYEQYDFVVPTRTDGDTYARY
                     LVRIEEMRQSLLIVQQALDKLPYGPVRSNNRKFVPPPRSELGVSMEALIHHFKLWTEG
                     FNAPKGGVYIAVESPRGELGVYLEGDGGPKPHRVYWRTPSFVNLQSLPLLSKGHLVAD
                     LVALIGTLDVVLGDVDR"
     gene            complement(3521..4033)
                     /locus_tag="AC16CB_02870"
     CDS             complement(3521..4033)
                     /locus_tag="AC16CB_02870"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR3341440.1"
                     /translation="MDEHLQSAAQAIQERFGANISEYRGDVSLLVSPDQIVEVCRALR
                     DEFNFEMLSGETAVDYWPELEPRFHLVYLLYSLVNNVRIGLRVPLNGNAPSLPTLESV
                     YPNANWHEREIWDMFGIRFEGHSDLRRILMPYEWEGHPLRKDYPLGYEEVQFTFNYDE
                     IDARKPYAKE"
     gene            complement(4036..4518)
                     /locus_tag="AC16CB_02875"
     CDS             complement(4036..4518)
                     /locus_tag="AC16CB_02875"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HIR3341441.1"
                     /translation="MGIEQKLGNMGVVTIKLEQAVNWARTNAMWPMLFGLACCAIEMM
                     SAQASNYDMSRFGMELMRASPRQSDLMIVAGRVSRKMAPVLRRLYDQMPDPKWVVSMG
                     DCASCGGVFNNYAIVQGVDEVVPVDVYVAGCPPRPEALIHGIMTLHEKVRGEKIKDWA
                     "
     gene            complement(4509..4865)
                     /locus_tag="AC16CB_02880"
     CDS             complement(4509..4865)
                     /locus_tag="AC16CB_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR3341442.1"
                     /translation="MLTQYLPIAILVVLATGLAFLVIALGHLFGPRRPTERKSLPYES
                     GMIPIGPGTRRMPVRFYLVAVLFILFDIEVIFFLPWAIVLRQLKLFGLLEMFVFVFIL
                     LVGYFYAWKKGALEWE"
     gene            complement(4992..6611)
                     /locus_tag="AC16CB_02885"
     CDS             complement(4992..6611)
                     /locus_tag="AC16CB_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3341443.1"
                     /translation="MVKSSKSPKPPSEDRTQPHHEDRENGEITSPLSLDKMGDLLAEI
                     QEDSIIEEPDDTALTLEAEELESLVKEEPQDILDDPKLIMELSDDPVRLYLKEIGGIK
                     LLDADEEFWLSTRLETVRRIDSLSRSHPLARRGTSVPKNIYRALFDELYFAWKRVQED
                     VKRLKHSAPDLLLVLAEAQTLRRSWNIQEPSYLRSFLDNGLWGTDPLWDGVARNAFTV
                     YLCLDSLPDGLAEFLDQDLKKRGNLPKESALLRRIPEDDILKAELEDIQMRANEAHHA
                     VIRANLRLVVSVAKRYIGRGSSFLDLIQEGNIGLLRAVSKFDPTRGYKFSTYATWWIR
                     QSISRSIADQARTIRIPVHIFESINRLIRVQRRLVQQLGREPTNEELAVEAGYLDGAD
                     QQNIARAQAEGTPLPIDLRRRLARAVAKVNRILQAAEEPMSLESPVGSEDSSQLGDFI
                     EDEEALEPMDAAAREMLRESVKNALAVLSERERQVLELRFGLMDGKDHTLEEVGQYFN
                     VTRERIRQIESKALRKLRHPTRSRHLRDYLG"
     gene            complement(6622..8574)
                     /gene="dnaG"
                     /locus_tag="AC16CB_02890"
     CDS             complement(6622..8574)
                     /gene="dnaG"
                     /locus_tag="AC16CB_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR3341444.1"
                     /translation="MSTIDEIKARLDIIDLVSESVQLRRSGKNYTGFCPFHSNTRTPA
                     FVVFPETGTWRCFGQCNEGGDIFSYVMKKEGWDFAETLRFLADKAGVELKPVTPEEQA
                     AAEEHDSLRLLLEEAVTFYRHQLLNTPAGQQALAYLHQRGLNDETIEAFDLGYAPQSW
                     DATLSYFKSKNYTDDDLLAVGLLTQSSQSEAEGGSQHPARIYDRFRHRIMFPIRDERG
                     RMAGFGARILDPNDIPKFLNSPQTAIFDKGQLLYGLDRARKPIRKVDQAVIVEGYLDV
                     IALHQAGFDNAISPMGTALTERHLYLLKRYTRRIVMALDPDAAGSQATLRGLQIARQS
                     LDREQELVFDARGLLGFEARLQADIRVTTLPEGMDPDDVVNRDPQEWQRILEHAKPVV
                     LHVMETLAAQQDLDDPKVKSQIAAQVLPLINDLPSAIERDTYRQRLARILKLDERTLS
                     GEVRSSRLRPPRRAPQKLKTAEPVETPDTSKTTSVSHRREAHALGVLLRRPDLLYQVN
                     RHLQESGLSRLTEDDFQHADHQAILHLLLESVDQDVAEPLNYVLNSLSLDLMEVADRL
                     IAPTAKLDPNDERVLADLVRAILDLRERHLKQFINHLRFLQEEAQQSGDLMAVEYGQI
                     VVTNSRILNQLNKALGKYSDRVIVNR"
     gene            8652..9209
                     /locus_tag="AC16CB_02895"
     CDS             8652..9209
                     /locus_tag="AC16CB_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010565120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR3341445.1"
                     /translation="MMTTPQNSPEGYISVDVETAGPNPSQYSLLTIGACTLSGSPRTF
                     YVELKPVNMNAIPEALAASRLSMEHLAERGLEPAEAMLRFEDWLKVEVPSGQKPLFVS
                     FNAPFDWMFVNDYFYRFLGRNPFGHTAVDIKAYYMGLTGVTWDQTSMKILGPRYLGNR
                     ELTHHALRDAMDQAELFRKMLEEKR"
     gene            9584..10903
                     /locus_tag="AC16CB_02900"
     CDS             9584..10903
                     /locus_tag="AC16CB_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015209715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341446.1"
                     /translation="MDPTPTNDLDRIIASAHRLGVEMDEEEALQWLTAIAASQAGDED
                     VVVDIHRGVFGHRVSMLDFDPKDLEHFREIGRLVEFQDVPDMVETALALSGSAAQSKI
                     QSFPGDCDYFERVNIKAATHAEACQILANLMREKALSTERGPTYQLIEVKFGSYPYDV
                     VVDGHPIKAGSPVSWTPEKIRDGQIGAFLPDGKPTAVRWEDVQTQPGWCKLDWVVADP
                     VRQQLANASNMLDVTWEAPDGTITPLDGYLDPYFQEVYLEAESIPIFSKLARHVSTDA
                     LDQYVAQLEKEVNKYLTKDVNYGKAAKRMYNVFRLTGRYEEAAFLRELFDEPTTLLYQ
                     IWSLIRTIDDCFKPNSSIGIEDLLTQTDQLILAIIKTLEGDQEAEIVRLLLRLRDSLS
                     RQDDTQILNAQSEAARAEVINVVNNFFHERLMAVPTIKSYIESMSTK"
     gene            complement(10911..13964)
                     /gene="glyS"
                     /locus_tag="AC16CB_02905"
     CDS             complement(10911..13964)
                     /gene="glyS"
                     /locus_tag="AC16CB_02905"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HIR3341447.1"
                     /translation="MTKTLDFQSIIMALQDFWAQQGCLIWQPYYTQVGAGTMNPATAL
                     RVLGPEPWNVAYVEPSIRPDDGRYGENPNRMQMHYQFQVILKPDPGNPQEIYLKSLQA
                     IGIDPRQHDIRFVEDNWESPALGAWGLGWEVWLDGQEITQFTYFQQAGGQILDPVSVE
                     LTYGLERIAIALQRVGSFREIQWSPTLTDGDVNLQAEQEHSQYYFEVADVNRLRQMYD
                     VFEAEAEACLAKNLVLPAHDYVLKCSHTFNVLDTRGAIGVTERAAFFGRMRDLSRRVA
                     EAYIEQRQRLEFPWLNHLETGETTVPAPHSSSPITYSLSPATFLLEIGTEELPSRDLD
                     AAIEQLRSRMAAMLDTLRLEHGELLVQGTPRRLVVYVEQLAPRQSDLEQVVKGPPAER
                     AFNPDGTPTQAAEGFARSKGIAVSDLQVREMDGGRYVVAVVRQEGLPAHSVLAEALPD
                     LIASLRFDKSMRWNSTNVAFSRPIRWLLALLGDQVVPFSYAGLGAGRSTRGLRFHAPA
                     EISLKTTQDYFNALKAQDIILDVEERRTYIQNQIESLAAEVGGVIAPDSDLLAEVANL
                     VEAPIALRGSFDPSHLELPREVLISVMKKHQRYFPVFEASESTDETAPDIQKPVQPLL
                     PYFVAIRNGDDLGLNLVVEGNEHVIRARFADAAFFVRDDSKRHLQEYLPQLKTLSFQK
                     KLGSMLDKTQRISEMLTDLAPLVGLSRDEMAVAGRAAELCKADLATKMVVEMTSLQGL
                     MGRHYALISGEPSEVANAIFEHYLPRFSGDEIPGERPGLVVGLADRLDTLAGLFAAGL
                     APTGNKDPFAQRRAALGLVQNLIAWELPFDLRQALELAAKRLPIPASHESQKACLEFI
                     IERLRNLLLEQGWRYDVVDAVLAEQGHNPARAAQAVKSLAAWVSRPDWSTILPAYARC
                     VRITRDQAEHFPVDRSKFVERAEGELFSALLTAESASAENRQHGSPDIFLNALLPMIP
                     AIDQFFDDVLVMTEDIAIQHNRLGILQRISALADGVADMSRLEGF"
     gene            complement(14092..14406)
                     /locus_tag="AC16CB_02910"
     CDS             complement(14092..14406)
                     /locus_tag="AC16CB_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR3341448.1"
                     /translation="MIPSLRDWHESYSPEGLVVIGNHYPEFDYEADLENLKEAVKALE
                     VPYAVAQDNDGETWRAYHNRYWPTLYLIDKWGHIRYRHIGEGRYDETEAAIQALLKET
                     FP"
     gene            complement(14422..14586)
                     /locus_tag="AC16CB_02915"
     CDS             complement(14422..14586)
                     /locus_tag="AC16CB_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341449.1"
                     /translation="MDTFTSPGGPFRADLDDLGMAPELDNEVWLNTESPLRLADLRGK
                     VVLLDMWTFG"
     gene            14788..15036
                     /locus_tag="AC16CB_02920"
     CDS             14788..15036
                     /locus_tag="AC16CB_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIR3341450.1"
                     /translation="MVMPVIQYAPETCAWCEGSGKYGAYNDACLVCGGQGSLLVAQPA
                     RKCPWCEGKGEVQTEEFRDRCKMCGGAGWSHVFKQASR"
     gene            complement(15082..16131)
                     /gene="tsaD"
                     /locus_tag="AC16CB_02925"
     CDS             complement(15082..16131)
                     /gene="tsaD"
                     /locus_tag="AC16CB_02925"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR3341451.1"
                     /translation="MSQSPPPPEALPLARVLGIETSCDETAAAVVENGRLILSNLVAS
                     QVELHAQFGGVFPEVASRQHIRTIYAIVEGALQQAHLDLRDVDAIAVTRGPGLPGSLV
                     VGMNVAKGMSLGSGLPLLGINHLEAHIYSAWLYAADAAPVAEPRFPLVVLIVSGGHTE
                     LILMTDHLQYRRLGGTLDDAAGEAFDKVARLLGLGYPGGPSIQKSAEIGNPRAFDLPR
                     AWLEDTWNFSFSGLKTAVLREVHRIQERGRPVPVPDMAASFQAAVVDVLVGKTLRAAE
                     SFKANDILVAGGVSANRALRQEFTTQSRFRVHIPPLWLCTDNAAMIAGAGYYRYIHEQ
                     RDGLEMDVLPNWALG"
     gene            complement(16155..>16610)
                     /locus_tag="AC16CB_02930"
     CDS             complement(16155..>16610)
                     /locus_tag="AC16CB_02930"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3341452.1"
                     /translation="GYFYDLTRTWCLGHATDEAQALYEDVLAVYTQVLSELKPGIHYP
                     RFQQRACELFEARGHPTQHSDPQTQRGYVHSLGHGVGMDIHERPFSGRSAAPDDTLQP
                     GSVVSHEPGLYYPERKLGVRLEDTLWMRTDGSVEILAEYPLELVLPVKP"
BASE COUNT         3882 a         4486 c         4549 g         3693 t
ORIGIN      
        1 ccatggtcag gttctgtagc gggcactcgc cgcccttatc gcagatgggg caatcgagag
       61 ggtgggaggt gagcaggaac tcaaggatgt cctcgcggcc ggccttgact ttatcagaca
      121 taccaacaac gaccatccct tccgagacgg gtgtcgtgca ggcggtctcc agcttaggac
      181 caaactggat ctttggcgag ccatcggctt ctaaaaccgg ttgtcctgtg gcgcggtcaa
      241 tcactgggcg gccgatatcc accagacaca tacggcacat tcccacaggc tccatcttgg
      301 gatggtaaca aaataccggg atgtcaatgc cgatcttttt agcggcgttc actagcagtg
      361 tgccagcagg cacggagact tgcaacccat caattgtcag tgttaccagt ttgctcatta
      421 aagatactcc aatgaaagcg gatcgcaatc ggaggccagc ccatttgccc tcgatgtgaa
      481 gcaattatgg cgtttgggct gattcctcac tctggacaac ggattgctga ccacctgtct
      541 gcaaatcaaa atcatccttg aagcggtcaa tccccgaaag caccgcttca attgaaaact
      601 cacccagggc acataagcat ttccccttga tctggctggc gacatcgtgg agcaactcta
      661 cgtcgttttg ggtggctttg ccatccgcga tacggtctgt gaggtgcatc atccaatatg
      721 tgccctctcg acaaggggtg cacttgccac aggattcgtg cttgaagaag tggattgtct
      781 tattaataag ccaggggata ttaaccgaat catcgatgac gataattgaa gctgagccaa
      841 gttgcgcgcc aacgctgggc acgctctcgt aatccatggg cgtgtccaga gcctgatccg
      901 ttgctacgat gagcgaggaa gaggcgccgg ccggcatgat ggccttgatt ttgttgcggt
      961 tagggattcc gccgccgtgc tcaaagatca attcgcggaa agtagtaccc agcggcaact
     1021 cataattgcc aggacggttg accttgccgg agagcgaaaa gatcttcacg ccgggacttt
     1081 tctcagtgcc gtaagtgcgg taccattctg cgccgcggtc taggatcaat ggcacatttg
     1141 caagcgtctc cacattgttg accactgtgg gcttaccata caacccgtag gagggtggaa
     1201 agggaggacg aaggcgcggt tggccaagtt tgccctccag cgattcgagc aaggcggtct
     1261 cttctccgca tatatatgct ccagcaccca ggtgggtata gatatgcagg ttgtagcctg
     1321 taccgaaaag attatctcca agcaaaccag ccagctctag cgcagcgata ttttggtcaa
     1381 gctggctagc aatttgccag aactcgccgc gcaggtaaac ataggccgcc ttcgcctgta
     1441 cggcgtaagc agcgatcgct acaccctcca aaaactggta ggggttgcct tccataatct
     1501 cgcgatcttt gaaggtaccc ggttctgatt cgtcggcatt tgcgaccaca taatgtggcc
     1561 aggatttttt atcaacaaaa gcccacttca gcccggtggg gaaacctgcc ccgccccggc
     1621 cgcgaagccc tgaggctttg accacctcga tgacctcgtc aggctgcatc gaggtgacga
     1681 ccttatgaaa ggcctggaag ccgccatgct ggcgataaac ctccaaccga ttgatggtgg
     1741 gtagctcacg gttgcgaaga agcaggtatt tattcatgcc cgcccctcct ctttacgcca
     1801 agacttgacc aagttcatgg cgctttcaac cgtctgattt tcatgatagg tcaagccatc
     1861 gccagactgc acctggaaca tgggggcttt atcgcaccca gccaagcaca taaccgcttc
     1921 tactgtcacc agcccgtcgg gtgtggtctc tcccacgcgg atgcccaggt tttggcagag
     1981 attctccaga aattcatcgg cgccacgcag ggcgcagggc aggtcggtgc aaacctggag
     2041 atggtactta cctccgactt cgtcataata aagtgtgtaa aaaccgacaa tagaagccac
     2101 atcggttgaa gatatgccca atatttcggc gatctcgctc attgattgct tggtcacata
     2161 gccctgctcg cgctgtgcta ggtagagcaa cggcattact gctgagcgct tttgatccga
     2221 ggggtatttg gctaggatag tgcggatttc ttctggatac ttctcggtca gcatgtgccc
     2281 tcctcttatc accggtcgac gtcaccgagt accacgtcga gggtgccgat taaagccacc
     2341 aggtcggcga ccagatgccc ttttgacagc aagggcagag attgcaaatt tacaaaagaa
     2401 ggcgtgcgcc agtagacgcg atggggtttg ggaccaccgt cgccctccag gtacacaccc
     2461 agctcgccgc gaggcgactc aaccgcgata tagacgccac ctttaggggc gttgaagccc
     2521 tcggtccaga gcttgaaatg gtggatgagc gcttccatgc tgacgcctag ctctgaacgt
     2581 ggtggcggga cgaactttcg gttgttgctg cgcaccgggc catagggaag cttatctagc
     2641 gcctgttgca caatgagcag cgattggcgc atttcttcga tacgcaccag gtaccgcgcg
     2701 taggtatcgc catcggtgcg ggttggaacg acaaaatcgt actgttcata tcctgaatag
     2761 ggctgggctt tgcgcaggtc gtgcgccact cctgaggcgc gcaggcttgg gccagttata
     2821 ccccagccaa tagcatcctg gcgggatagt acaccgatct ttttagtccg ctcaatgaac
     2881 aggggattct tggtgaggag cgcctcgtat tcatcaatgc gagcggggaa aacacgcaag
     2941 atttggcgca cggcctcctc aaactcaacg gggatatccc gccataggcc gcccggacgg
     3001 aagtaagtag acatcatgcg ctgaccaccg cacatctcga ggacgtccag gatcatctcg
     3061 cgctcgcgga agcagtaaag aaaaaccgac atcgccgcca ggtcaagggc gtgcgtgcct
     3121 aaccagacga gatgggaagc aatgcgttgc agctcggtaa ggatgacccg gatagcctgg
     3181 gcgcgcaggg gaacatccag atccaccagt ttttcaatag ccaagcagta gaccaagtta
     3241 ttgcccgttg tgttgagata atccaagcgg tcggtcatta ccgctgcttt ttcatacgtc
     3301 ttggcttcca tgtttttctc aatgccagtg tgcaggaagc caacatctgg gatgcagttg
     3361 accacgatct caccgtccag ctctaacagc aagcgtagta ctccgtgcgt gctgggatgc
     3421 tgcggcccca tgttgagcaa cacggtttcg ccttcgatag cccgagctga gactaggtgg
     3481 cgtagctcat cgagtgagac ttcagttttt tccaacatgc ctattccttc gcgtagggct
     3541 ttcgagcgtc aatttcatcg taattaaatg tgaactgcac ttcttcatag cctaagggat
     3601 agtctttgcg cagggggtgc ccctcccatt catagggcat gagaatgcgg cgtagatcgg
     3661 agtgaccttc gaagcgaatg ccaaacatat cccagatctc gcgctcatgc cagttagcgt
     3721 ttgggtatac gctttcgagg gtaggcaagc tgggggcgtt gccattcaat ggcacgcgca
     3781 ggccgatacg cacattattc accagcgagt agagtaggta aaccaaatgg aagcgcggct
     3841 ccagctcagg ccagtaatcc actgcggtct cgccagaaag catctcgaaa ttgaactcgt
     3901 cgcgcaacgc acggcagact tcgacgatct ggtcaggaga gacaagcagg ctgacatcgc
     3961 cgcggtactc gctgatatta gcgccaaagc gctcttgtat cgcttgagca gcagattgta
     4021 agtgttcgtc catgattaag cccaatcctt gattttctcg ccgcgcacct tttcgtgcag
     4081 ggtcataata ccgtgaatca gggcctcagg gcgcggcggg cagcccgcca cgtaaacatc
     4141 cactggcacc acttcatcca caccttgtac aatggcgtaa ttattgaaaa cgccaccaca
     4201 tgaggcgcaa tcacccattg agaccaccca cttgggatca ggcatctggt catacaggcg
     4261 gcgtaaaaca ggcgccatct tgcggctgac gcgccccgca acgatcatca aatccgattg
     4321 gcgcgggcta gcgcgcatca gttccatgcc gaagcggctc atgtcgtaat tggaagcctg
     4381 cgctgacatc atttcgatgg cgcagcaagc taacccaaaa agcatcggcc acatggcgtt
     4441 ggtgcgagcc cagttgacag cttgctcaag tttgatggtc accacgccca tgttgcccag
     4501 tttttgctct attcccattc cagcgctcct ttcttccacg cataaaagta acctaccagt
     4561 aatataaaga cgaagacaaa catctcaagc aatccgaata gcttcaactg gcgcaggacg
     4621 atagcccaag gcagaaaaaa gatcacctca atatcgaaca ggataaacaa caccgccacc
     4681 agataaaacc gaactggcat tcggcgagtg ccaggtccga tgggtatcat tccagattca
     4741 tagggaaggg acttgcgttc tgtggggcgt cttgggccga agaggtggcc tagtgctatg
     4801 accaagaagg ctaaaccagt agccagaacg actaaaatgg cgatggggag gtattgcgtc
     4861 agcatagttt gcctcgaaag tgaattcgtc gattgttaat ctaatctggt ctaagttgta
     4921 agtttatcac aggtggtaaa tttcacaaat taaaaataga taaatgacca gtttgccaaa
     4981 aacgcaacat gtcagcccaa ataatcgcgt aaatggcggc tacgcgtagg atggcgcaac
     5041 ttccgcaatg ccttggattc aatctggcga atgcgctcgc gggtgacatt gaaatattgc
     5101 cctacttctt ccagtgtatg gtccttgcca tccatcaggc caaaacgcaa ctcgagcacc
     5161 tgtcgctcgc gctctgagag caccgccagt gcattcttta ctgattcgcg tagcatctca
     5221 cgcgccgcgg catccattgg ctctagggcc tcctcatctt caatgaagtc accaagctgg
     5281 ctgctgtctt cgctgccgac cgggctttcc agcgacatcg gttcttcagc ggcctgcagg
     5341 atgcggttca ccttagccac cgcacgcgcc agcctgcggc gaaggtcgat tggcaggggc
     5401 gtgccttcgg cctgggcgcg ggcaatgttc tgctggtcgg ctccatcaag atagccagct
     5461 tcaacagcca gctcctcgtt ggtgggttcg cggcctagtt gctggactaa tcgcctctga
     5521 acgcgaatca ggcggtttat agactcaaag atgtgtacag ggatacggat ggtgcgcgcc
     5581 tggtcagcga ttgatcggct gatagactgg cgaatccacc aggtggcata ggtactgaat
     5641 ttatagccgc gtgtcggatc aaacttggat acagcccgca gcaatccaat gttcccttcc
     5701 tggattagat ccagaaaaga actgccccgc ccaatgtagc gtttggccac gctaactacc
     5761 aggcgcagat tggcgcgaat gacagcatgg tgggcttcgt ttgcgcgcat ttggatatct
     5821 tcaagttcag ccttcaagat atcatcttcg ggtatacgcc gcagtagagc gctctcctta
     5881 ggcaaattac cacgcttctt gagatcttgg tccaaaaatt cagccagccc atccgggaga
     5941 gaatctaggc agagatagac ggtaaaggca ttgcgtgcca cgccgtccca aagcgggtcc
     6001 gtgccccaaa gtccgttgtc cagaaaactt cgcaggtatg aaggttcttg gatattccat
     6061 gatcggcgga gagtctgggc ttcggcgagt acaagcagta aatccggagc actgtgcttc
     6121 agccgcttga catcttcctg tacgcgcttc caagcgaagt acagttcgtc gaagagagcc
     6181 cgataaatat ttttcggtac agaggtgccc cggcgcgcca gagggtggct gcggctgagc
     6241 gaatcaatac gccgcactgt ctccaggcga gtagatagcc aaaattcttc gtctgcatcc
     6301 agcaatttga tcccgccgat ctccttgagg tacaggcgaa ccggatcatc gctcaattcc
     6361 atgatcaatt tcggatcatc caggatgtcc tggggctctt ctttaaccaa actttccaat
     6421 tcttctgcct ccagggtgag agcggtatcg tctggctctt caattattga gtcttcttgg
     6481 atctcggcga gcaaatcacc catcttatct agcgaaagag gcgaagtgat ctccccattt
     6541 tctcgatctt catgatgagg ctgggttcga tcttccgacg gtggtttagg cgatttagaa
     6601 ctttttacca ttacaattta ctcagcgatt tacgatgaca cggtcagaat acttaccaag
     6661 cgccttattc aattggttca aaattcggct attcgtaacc acgatctgcc catattctac
     6721 cgccatgaga tcccctgact gctgcgcctc ttcttgaaga aagcgcaagt ggtttatgaa
     6781 ctgctttaga tggcgctcgc gcaggtccaa gatggctcga accagatccg caaggacgcg
     6841 ttcatcattt ggatccagct tggcggtggg tgctataagc ctatcggcga cttccatcaa
     6901 gtccagggag aggctattga gcacatagtt aagtggctca gccacatcct gatccacgga
     6961 ttcgagcaac aagtggagta tcgcctggtg gtcggcatgc tgaaaatcat cctcagtgag
     7021 gcggcttagc ccgctctcct gcaggtggcg attgacttgg tagagtaaat ctggacgacg
     7081 tagcagcaca cccagggcgt gagcttcccg tcggtgggaa actgaagtgg ttttagaagt
     7141 gtccggggtc tcaacgggct cggcggtctt gagcttttga ggcgctcgtc gaggcgggcg
     7201 caatctggaa ctgcgcacct cacccgagag ggtgcgctca tctagtttga ggatgcgggc
     7261 aaggcgctgg cggtaggtat cgcgctcgat ggcgctgggc agatcgttga tcagtggtaa
     7321 cacctgggca gcaatctggc tttttacttt ggggtcatcc aaatcttgtt gagcagccaa
     7381 tgtctccatg acgtgcagaa cgaccggctt agcatgctcg aggatacgct gccattcctg
     7441 cgggtcacgg ttcacgacat cgtcgggatc catgccttca ggcagcgttg taacccggat
     7501 atccgcctga aggcgagcct caaagccaag cagaccgcgg gcatcaaaga cgagctcctg
     7561 ctcacgatcc agcgattggc gagcgatttg tagcccgcgc agcgtagcct gactgcccgc
     7621 cgcgtcggga tctagcgcca tgacaatgcg gcgggtgtag cgcttgagca agtacaggtg
     7681 gcgctcggta agagcagtgc ccatcgggga aatagcattg tcaaagccag cctggtgcag
     7741 ggcgattaca tccagatagc cttctacgat gacggcttga tccactttgc ggatgggctt
     7801 tcgagcgcga tccaatccat aaagcagttg gcctttgtca aagatagctg tttgggggga
     7861 attcaggaat ttgggtatat cgttggggtc caagatacgc gccccgaagc cagccatgcg
     7921 cccacgttca tcacggatgg ggaacatgat gcggtggcga aagcgatcat atatcctggc
     7981 gggatgctgg cttccccctt cagcctcact ctggctcgac tgggtgagca gacctacggc
     8041 cagcaaatca tcgtccgtgt agttcttgga tttgaaatag ctcagcgttg catcccaaga
     8101 ctgcggggca tatcccaggt cgaaggcttc gattgtttca tcgtttaggc cgcgctggtg
     8161 caggtaagcc aacgcctgct gaccagcagg cgtattaagc aattggtgcc gataaaatgt
     8221 gactgcctct tcgagcaaaa gacgcaagct atcgtgctct tccgcagcag cctgttcttc
     8281 tggggtgact ggcttaagtt ccaccccagc tttgtctgcc aggaagcgta atgtctccgc
     8341 aaaatcccag ccctctttct tcatcacgta gctaaagatg tctccccctt cattgcactg
     8401 accgaagcag cgccaggtgc ccgtttcagg gaaaactaca aatgctgggg tgcgcgtatt
     8461 gctgtgaaat ggacaaaacc ctgtataatt cttgccagag cggcgcagtt gcaccgattc
     8521 cgagacaaga tctataatat ctaggcgggc ttttatctcg tcaattgtac tcatgggctt
     8581 atacgattga gcatgtaaat tataccagca ggagaatatt tcatcctgcg aatggactgg
     8641 tttataatca gatgatgact acaccccaaa actctcccga aggttatatc tcggtggatg
     8701 tcgagaccgc cgggccgaat cccagtcaat attcgctgct gacaattggc gcctgcacct
     8761 tgagtgggag cccgcgcacg ttttacgtgg agctcaagcc ggtcaatatg aatgcgatcc
     8821 cagaggcact ggcagccagc cgtctttcca tggagcacct ggctgagcga ggactggaac
     8881 ccgccgaggc gatgctccgc ttcgaggact ggctaaaggt tgaggtaccc agtgggcaaa
     8941 agccgttgtt cgtctccttc aatgcgcctt ttgattggat gtttgttaac gattattttt
     9001 atcgattcct cgggcgcaat ccgtttggac atactgcggt ggatattaag gcctattaca
     9061 tgggtctgac gggtgtgacc tgggatcaga catccatgaa aatcctcggt cctcgctacc
     9121 tgggaaacag agaacttacc catcacgcct tgcgtgacgc catggaccaa gccgaactct
     9181 tccgaaagat gctggaagaa aaacgttgat cagttatcaa taattggaga caagaggccg
     9241 tccaataagg tatttgtggc ggtcggggtg attaaatcaa gagcggtcag atggctggca
     9301 attcgatcga caaagggggt gtaggaatat tcggttaaat aacccaatag cctcgatatt
     9361 tgagaagttc attagcccta cccttgaaga gtaacgaaga gaaaaaagga acgtttatta
     9421 ttacagtagt tagcttcggt gcacaactac cgtttcgatc gtatggcaac ggtggttagg
     9481 gcacaatgtt ctttgagtga atcagacaat agatacgcgg tctggaagtc tttttgatca
     9541 accgcgtatg ccacatattt aacacgcatt taggagaaat aggatggatc caacgccaac
     9601 aaacgatctt gaccgaatta tcgcttctgc acaccgcctg ggtgtagaga tggacgagga
     9661 agaggcctta cagtggctga ccgctattgc cgcctcccag gctggcgatg aagatgtggt
     9721 ggtggatata caccgtggtg tattcggcca tcgggtgagc atgttggact ttgatcctaa
     9781 agacctggag cattttcgtg agattggccg attggtcgaa ttccaagatg tgcccgatat
     9841 ggtcgagacc gcactggcgc tctcgggctc ggcggcgcaa tccaaaattc aaagcttccc
     9901 tggtgattgc gattacttcg agcgtgtcaa catcaaagct gcgactcatg ctgaagcctg
     9961 tcaaatcctg gctaacctga tgcgcgaaaa agcattgagc actgagcgcg ggccgaccta
    10021 ccagctcatc gaggtcaagt tcggatcgta tccatatgat gtggttgtgg atggacatcc
    10081 tattaaggct ggatcgccgg tttcctggac cccggaaaaa atccgagatg ggcagattgg
    10141 cgctttcctc cccgatggca agcccaccgc ggtacgctgg gaagatgtgc aaacgcaacc
    10201 gggctggtgc aagctggatt gggtggtagc tgatccagtc cgccagcagc tggcaaatgc
    10261 tagcaatatg ctggacgtca cctgggaagc gccagacggc accatcaccc cattagatgg
    10321 atacctggat ccgtattttc aagaagttta cctggaagct gagtctatcc ccatattctc
    10381 caagctggca aggcacgtct cgacagatgc cctagaccaa tatgtggccc aactggaaaa
    10441 agaggtcaat aaatacctga ccaaagacgt aaactatggc aaggctgcca agcgcatgta
    10501 taacgtcttc cggctgaccg ggcgttacga agaggcggct ttcctgaggg agttattcga
    10561 cgagccgaca accttgctct accagatctg gtcattgatc cggacaattg acgactgctt
    10621 caagcccaat tccagcattg ggatcgaaga cctgcttacc cagaccgacc agctcatcct
    10681 ggcgatcatc aagacccttg aaggagatca ggaagccgag atcgtgagat tgctgctgcg
    10741 cctgcgcgat agtctatccc gccaggatga cacgcagata ctcaatgcac agtcagaggc
    10801 ggctagggca gaagtgatca acgtggtcaa taacttcttc catgagcgac tgatggctgt
    10861 gccgaccatt aagtcatata ttgagagtat gtcaactaaa taagagcata tcaaaagcct
    10921 tctaggcggc tcatatccgc tacgccatcc gccaatgcag atatccgctg caggatcccc
    10981 aggcggttgt gttgaatggc aatgtcttcg gtcatcacaa ggacgtcatc aaagaactgg
    11041 tcgatggccg ggatcatggg caggagagcg ttcaggaaaa tatccggtga tccatgctgc
    11101 ctattctccg ctgatgcgga ttcggctgta agcaaagcgg agaagagctc accttcggcg
    11161 cgctcgacga acttgcttcg atcgaccggg aagtgctcgg cttggtcgcg ggtgatgcgc
    11221 acgcagcggg cgtaggcagg caagatggta ctccaatctg ggcgtgacac ccaggcagcc
    11281 agtgatttga ccgcctgagc tgctcgagcc gggttgtgac cctgttcagc cagcacagca
    11341 tcgactacat catatcgcca gccctgttcc agcaacaggt tccgcaagcg ctcaatgata
    11401 aactccaggc aagctttttg actctcatgg cttgcaggga tgggcaggcg ctttgcagct
    11461 aactccagag cttgtcgcaa atcgaagggc agttcccaag ctatcaggtt ctgtaccaaa
    11521 cccagggcgg cgcggcgctg agcgaaggga tctttgttgc cagtgggcgc taacccagcc
    11581 gcaaataggc ctgccaatgt atccagtcgg tctgccaacc caaccactaa accagggcgt
    11641 tctccaggaa tctcgtcgcc cgagaaacgc ggcaggtaat gctcaaaaat ggcatttgcc
    11701 acttcactcg gctcacctga gatcaaagcg tagtgacggc ccatcaaccc ttgcaatgaa
    11761 gtcatctcaa cgaccatctt ggttgccagg tcagccttgc ataactcggc ggcgcgtcca
    11821 gcgacagcca tctcgtcacg gctgagcccc accaggggag ccaggtccgt caacatttcg
    11881 ctgatgcgtt gcgtcttatc cagcatcgag ccgagcttct tctggaagga gagagtcttc
    11941 aactggggaa gatactcttg caggtggcgc ttgctatcgt cacgcacgaa gaaggctgcg
    12001 tcggcaaagc gggcgcggat aacatgctcg ttgccctcca ccaccaggtt caacccaagg
    12061 tcatcgccgt tgcgaatggc tacgaaataa ggtaataatg gttgcactgg tttttgtata
    12121 tctggcgccg tttcatcagt cgactcggaa gcctcaaaaa cggggaaata tcgctggtgc
    12181 tttttcataa cggaaatcag cacctcgcgg gggagctcta gatgagatgg atcgaaggag
    12241 ccacgcagcg caatgggggc ttctaccagg ttggcaacct cggcaagcag gtcgctatct
    12301 ggtgcaatta ccccgccgac ctctgccgct aaactttcga tttgattttg aatgtatgtg
    12361 cggcgctctt ccacatccag gatgatgtct tgtgctttca aagcgttaaa gtaatcctgg
    12421 gtggtcttca agctgatctc agccggcgcg tgaaaacgca gacctcgcgt ggaacggcct
    12481 gcgcccaaac ctgcatacga gaagggcact acctgatcgc ccaacaaggc aagcagccag
    12541 cggattgggc gggaaaaagc cacattggtg ctgttccagc gcatcgactt gtcaaaacgc
    12601 agtgaggcga tcagatcggg caatgcctct gctaacacgc tatgggctgg gaggccttcc
    12661 tgccggacca ccgcaacaac atagcgcccg ccgtccatct cccggacctg caggtcgctc
    12721 accgctatcc ccttactgcg agcgaaaccc tcggcagcct gggtgggggt gccatcggga
    12781 ttgaaggcgc gctcggcagg cggacctttc actacttgct ctaggtcgct ctgacgtgga
    12841 gctaattgtt ctacataaac aaccaggcga cgcggcgtgc cctggacgag cagctcgcca
    12901 tgttcaagac gcagcgtgtc caacatggca gccatacgag agcgcagttg ctctatcgcc
    12961 gcatccagat ctcgggaagg aagctcttcc gtgccgatct cgagcaagaa agtggcaggg
    13021 gagagagagt aagtgatagg ggacgatgaa tgaggggcag gcactgttgt ttcccctgtc
    13081 tctagatgat taagccaggg gaactcaagg cgctgtcttt gctcaatata ggcttctgcc
    13141 accctgcgcg acaggtcgcg catgcgtccg aagaaagccg cgcgctcagt gacgccgatg
    13201 gcgccacgcg tatccagcac attgaacgtg tgagagcact tcagtacata atcgtgggca
    13261 ggcagcacaa gattctttgc taggcaggcc tccgcctcag cctcgaacac atcatacatc
    13321 tggcgtaggc ggttgacatc agcaacttcg aagtaatatt ggctgtgttc ttgctcagct
    13381 tgcaggttca cgtcgccgtc ggtgagcgtt gggctccact gtatctcgcg gaagctgccc
    13441 acgcgctgca gggcgattgc aattcgctcc agcccgtagg taagctccac cgagactgga
    13501 tccagaattt gcccacctgc ctgctggaaa taggtgaact gggtgatctc ctgtccgtcc
    13561 agccagacct cccagccaag tccccaggca cccagggcag gcgattccca attatcctct
    13621 acaaagcgga tgtcgtgctg gcgcgggtca atgccaatcg cctgtaatga ttttagataa
    13681 atctcctggg ggttgcctgg atcaggcttg aggatcacct ggaactggta atgcatctgc
    13741 atgcgattgg gattctcgcc ataacgacca tcgtcgggac ggatagacgg ctcgacatag
    13801 gcgacattcc acggttctgg tccaagcacg cgcagcgcag tcgctggatt catcgtacca
    13861 gcgcccacct gggtgtaata tggctgccag atcaggcatc cctgctgagc ccaaaagtct
    13921 tgcaaggcca tgataatgga ttgaaaatca agagtttttg tcattgagtg tagtaaatcg
    13981 cagatactgg tgtgaatatt cgcccgaatt atacacgtaa aaatgggcag catctaaatg
    14041 ctgcccgtaa atgatgatgg gtcttgtagc accttgggga ggtcacgcgt tctaggggaa
    14101 tgtttccttt aggagcgcct gaatagccgc ctcggtctcg tcgtaccttc cttcgccgat
    14161 atggcgatag cggatatgac cccatttgtc aatcaaatac agcgtaggcc agtagcggtt
    14221 atgatacgcc cgccaggttt cgccatcgtt gtcttgggcg actgcgtagg gcacttccaa
    14281 tgccttgact gcctctttta gattttcaag atcggcttcg taatcgaatt ctgggtagtg
    14341 gttgccgatc accaccagcc cctctgggct ataactttca tgccaatccc tcaacgaggg
    14401 aatcacgcga cggcaattga ttcagccaaa ggtccacata tcaagcagca ccaccttgcc
    14461 gcgcagatca gccaggcgca agggggattc ggtgttgagc cagacctcat tatccagctc
    14521 tggcgccatc ccgagatcat ccaggtcggc gcggaatggt cccccaggcg aggtaaatgt
    14581 atccatgatc tcaaccttcg ggttggtcag tgcagtaaca catccaacca acacaagtgt
    14641 aagcgttcct aataaccacc atctcacaag tataattctc cctgcaatgt gggaaattgc
    14701 tctaatgcga ttaccctatt ctatcaacaa ggtattacga aagaattacg cttggggagt
    14761 ataatccaaa gcaggaggat ttacccgatg gtgatgcccg taatccaata cgctcccgaa
    14821 acatgcgcct ggtgcgaagg tagcggcaaa tatggcgctt ataacgatgc ttgtctggta
    14881 tgcggcgggc agggaagtct cctggtggcg cagcctgcac gaaagtgtcc ctggtgtgag
    14941 ggcaagggag aagtccaaac agaagaattc agagaccgct gcaaaatgtg cggcggggca
    15001 ggctggtcac acgtattcaa gcaagccagc cgctaaacct tttccaggca cgttttagaa
    15061 gagggataaa tgggggagaa tctacccaag tgcccaattg ggcaatacat ccatctctag
    15121 cccgtcacgc tgttcatgga tatagcggta atatcctgcc ccagcgatca tggcagcatt
    15181 gtcggtgcac agccacaagg gcggtatgtg cacccgaaag cggctttggg tggtaaattc
    15241 ttggcgaaga gcgcggttgg cagagacgcc tcccgccacc aggatatcat tggctttgaa
    15301 cgactccgca gcgcgcaggg tcttgccgac cagcacatct accacagcag cctgaaaact
    15361 ggcagccata tcgggcacgg gcacgggccg gccgcgttct tgaatgcggt gcacctcccg
    15421 cagcacagcg gtcttcagcc cgctgaagga gaagttccag gtatcttcca gccaggcacg
    15481 tggcagatcg aaggcgcgcg ggttgccaat ctcggcagat ttctggatgg acgggccgcc
    15541 agggtagcct aaccccaaca agcgggcgac cttatcgaaa gcctcgccag ccgcgtcgtc
    15601 cagagtgccg cccaggcggc gatactgcag atgatcggtc atcaagatta gctcggtgtg
    15661 cccaccagag acgatcagca ccaccagcgg gaagcgcggt tcagccacgg gcgccgcatc
    15721 ggcagcatac aaccaggcgg agtaaatgtg tgcctccagg tggttaatcc cgagaagcgg
    15781 aagaccgctg cctagggaca tccccttggc tacattcatg cccaccacaa gggagccagg
    15841 cagcccaggc ccgcgcgtca cggcaatagc gtctacgtct cgcaggtcaa gatgggcttg
    15901 ctgcaacgca ccttctacga tggcgtagat ggtgcgaata tgctggcggg aggccacctc
    15961 gggaaagaca ccaccaaact gggcgtgcag ttccacctgg gaggcgacca gattggagag
    16021 gattaagcgc ccgttctcaa ccactgcagc ggcggtttca tcgcaggagg tttcaatgcc
    16081 cagaacacgt gccaggggta atgcttcggg gggtggtgga gattggctca tgattacctt
    16141 tccaactaat ttgcttatgg cttgaccggg aggaccaatt cgagtggata ctcagccaga
    16201 atctccactg atccatccgt gcgcatccac agggtatcct ccaggcgcac tcccagcttg
    16261 cgctcagggt aatacaaccc gggttcgtgg ctgaccaccg acccaggctg gagggtgtca
    16321 tctggtgcag cgctgcgccc gctgaacggg cgctcatgaa tgtccatacc caccccatgt
    16381 cccaggctgt gcacgtagcc tcgctgagtc tgcgggtcgc tgtgttgggt ggggtggccg
    16441 cgggcttcaa aaagctcaca ggcacgctgc tgaaagcgcg gataatggat gccaggcttg
    16501 agctcgctca aaacttgcgt atagacagcg agcacatctt cgtaaagcgc ctgcgcttca
    16561 tcggttgcat gccccaggca ccaggtgcgg gtgagatcat aaaaatagcc
//
LOCUS       DBNGSX010000087         7982 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1391788, whole genome shotgun sequence.
ACCESSION   DBNGSX010000087 DBNGSX010000000
VERSION     DBNGSX010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7982)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7982)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7982
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1391788"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..1029
                     /locus_tag="AC16CB_02935"
     CDS             88..1029
                     /locus_tag="AC16CB_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020481635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3341453.1"
                     /translation="MKSRIWLALIAVYIAWGSTYLAIRFAVESMPPFLMAATRFLIAG
                     MVVYIWRRMAGDPPPTRTQWRSAIIIGLFLLLGGNGGVTWAEQRIDSGMAALLVASAP
                     FWMVLIDALRPGGEHPNWQISAGVLIGLAGIAILVTPGQKLGSAIGFDLLGVTALMLA
                     SLFWAIGSIYGRGADLPKSPLLGTGMEMLAGSAGLFIAGTITGEWGRLELATITMRSL
                     SGLAYLIVVGSLVGFVAYSWLMRVAPMPIVSTYAYVNPLIAVILGSLLAQEELTVRVL
                     LAAPIIVSSVALINMARFKTTSRAPARLAVYAASGED"
     gene            1089..1733
                     /locus_tag="AC16CB_02940"
     CDS             1089..1733
                     /locus_tag="AC16CB_02940"
                     /inference="COORDINATES: protein motif:HMM:NF024247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3341454.1"
                     /translation="MMSTDHIKSFIRDLGIDLVGVADMTTLNGMPLGISAEGASFLDE
                     FPRAIVLGAQYGKIGKNAKGREVSFFLENAALQVLEYLEEEGTQGLIIHTEDEYNPIT
                     RQGLLSLKVLAKAAGLGWQGRSLLIVSPEYGPVHRWIAVLTDLDISPDQTLSNECGSC
                     SLCVDKCPQDALTLVPFEDHPESREAVLNVPACKGDNGCKVCIVVCPWFRQAIC"
     gene            complement(1723..2214)
                     /locus_tag="AC16CB_02945"
     CDS             complement(1723..2214)
                     /locus_tag="AC16CB_02945"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR3341455.1"
                     /translation="MKTGKFFLAFVALISILVTGCAKSTPEPVTYTIEMSEYSFSPNT
                     LELKVGQQVTINLVNKGTLAHEIMFGSEAMMMNNRPSGYQHDMFEESGSEPMVMGEMH
                     MEGEHDEGHTGFMVVLPEEGDTASITFTVTDEMVGEWEMGCFEQEGVHYDAGMKGKIT
                     ISK"
     gene            2439..3377
                     /locus_tag="AC16CB_02950"
     CDS             2439..3377
                     /locus_tag="AC16CB_02950"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR3341456.1"
                     /translation="MKQFKKNRIFELAKLGLPHIPAALGKFYPFLAIIYVLLGPVITS
                     AIAAIPIAIVFIISGSTHVDLQAIMGSSVGMTGLMLVGFLPIYLLVWGWLRVFEHRPL
                     WTIGMERKEWLSKYLRGALVGSVMISAAVGLPAMFGYIQLQGNSSGVSVATMGALILF
                     IGWVVQGAAEEVLFRGFLLQIIGSRFGVIAGVLVTSILFALLHIFNASLGVIALINLM
                     LFGVFAAFYTLWEGSLWGVFAVHSFWNWVQGNLFGMEVSGLPVRLEALIDLKEVGPDW
                     FTGGAFGPEGGIAVTLLLLAGIAVLWLLESRKQSES"
     gene            complement(3411..5315)
                     /locus_tag="AC16CB_02955"
     CDS             complement(3411..5315)
                     /locus_tag="AC16CB_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HIR3341457.1"
                     /translation="MDIDQQIILIEEIVIALLFVAVLVGIVAHRLRVPYTVGLVLMGL
                     TMTIWEQVDVQIAPNLILALLVPPLIFEAAFHLNINDLRRNLAPILALAIPGVIITTL
                     IVGGIVAWGTGLALPIAMVFGALVAATDPVSVVSLFRTMGVPKRLQVLLEGESLFNDG
                     TAIVVFDLVVVLAASEASHFNLASSVVEFVRVSGGGLVVGLLLGALISQMISRIDDYL
                     IETTLTSVLAYGAYLIAEILGVSGVLAVVAAGLINGNIGPRGMSPTTRIVVFNFWEYA
                     GFLANSFIFLLIGLQIHLPVLYQNWQMIVWAIVAILIARAIGVYGLSWFEKEIPPRWR
                     HVLYWGGLRGAISLALALSLPASLPQASPQLQAMAFGVVLFTLLVQGFTMKPLVTQLK
                     LTERSESQDEYERRHARAVATRHAYEHLSRHHSQGLLSDHSWDIIGPLLQEHNRTLAD
                     SVQEVVSSDPQVEQEELDTARREYLSAQRSAINSLRRDGTISDEIFNQLTAEIDGAIT
                     NPSAGWPELLSGNISQHTQVNRLMAVIVQEQDVENALSTLNKLGIPTTRLPSTGGFFR
                     RTNAILLIGIEKGQEASVVRALQLSCRKRVEYISAPIEGFPPGLPEPIPITVGGATIF
                     VFEVERFEIF"
     gene            complement(5385..6674)
                     /locus_tag="AC16CB_02960"
     CDS             complement(5385..6674)
                     /locus_tag="AC16CB_02960"
                     /inference="COORDINATES: protein motif:HMM:NF014135.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HIR3341458.1"
                     /translation="MTLPMTLSGIIFILSLYLIFSEKLNRTITSMAGAAVMIGAGLAF
                     NFYSEEQAVEAIDFNTVGLLLGMMILVALLEPTGFFQYLAIWATRWSGGNQMRLLITL
                     GTLTTLLSMVLDNVTTVVLIAPVTILISEILGINATPLLMAEALLSNTGGVSTLVGDP
                     PNVLIASAADFSFNDFLTHSLPVVLVAWLVALALISYLFRADLAKKPTNIKAIIDLNP
                     SEALTDRVTVGKVLIVLCATMILFFMEEILHTSPSFIALAAASVAVVWVQPDMAEMFK
                     RIEWSVLIFFCALFIMVGGLKSSGLLHSLVEVLTWGRTVNPAMFGVLLIWVIAILSAL
                     VDNVPITIALIPVIQDLELQGINVTPLWWALAFGAGFGGNATIIGSTANIVVATLSEK
                     THNPITSPLWNKRGLPVMIATCFVASVLYTLAFPLFQ"
     gene            complement(6671..7141)
                     /locus_tag="AC16CB_02965"
     CDS             complement(6671..7141)
                     /locus_tag="AC16CB_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3341459.1"
                     /translation="MEIKHCMKPQVVSIPETATIAEAATIVLEKRIGTLPVVDQDGVL
                     VGVLRLRDLVKLVMPDFIQLVEDFKFVHDFGAVETRLPSTATLSQSVLDIILDPISVD
                     ETCGLLRAAAIMYRQNLADILVVSADGKLVGIASHVDIGVALLSTWNISQGGMT"
     gene            complement(7152..7925)
                     /gene="fabL"
                     /locus_tag="AC16CB_02970"
     CDS             complement(7152..7925)
                     /gene="fabL"
                     /locus_tag="AC16CB_02970"
                     /EC_number="1.3.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabL"
                     /protein_id="HIR3341460.1"
                     /translation="MFTGKIALITGSGRGIGRAIALHLANLGADIVVNFFRNRRPAEE
                     TAEEIRALGRRSLVVKANVGEVDEINRLFETVEKEFSTLDILVSNAASGYNRPAMEQK
                     PKGWDWSMNINAHALLLSAQRAVPLMEQRGGGSIVAISSPGASRVLPDYIVVGASKAA
                     LEALVRYLAVELAPKNIVVNAVSPGIVLTNALKHFDNVKIEQSLIDKATEHIPAGRLV
                     TSQDVANVVAFLCSPQASMIRGQTILVDGGHSLPFQSIN"
BASE COUNT         1941 a         1913 c         2077 g         2051 t
ORIGIN      
        1 aaaaacgcta cgatacgccg cctacagctg tggtgcaact aaagactcag tccaactctg
       61 attgttgata aacgaaagtt gggatagatg aaatcgcgaa tctggctagc actgatagca
      121 gtatatattg cctggggatc gacctatctg gcgatacgct ttgccgtgga gagcatgccg
      181 ccatttctga tggcagccac acgttttcta attgcaggta tggtcgttta tatctggcgg
      241 cgcatggctg gcgacccacc tcccactcgc acccaatggc gctcggcgat aatcatcggt
      301 ttattcctgt tgttaggtgg aaacgggggc gtcacctggg ctgagcagcg cattgattct
      361 ggcatggctg ctctcttggt tgcctcggca cccttttgga tggtgttgat tgacgcattg
      421 cgtcctggcg gagaacatcc caactggcag atatcggctg gtgtgctaat cgggttagct
      481 ggcatcgcga ttttggttac tcccggtcaa aagttaggca gtgcgatcgg ttttgacctt
      541 cttggagtga ccgctttaat gctagcttct ctcttttggg ctatcggttc tatttatggt
      601 cgcggagccg atttacccaa atccccgctt ttggggacag gcatggaaat gcttgcaggc
      661 agtgccgggc tgttcatcgc ggggacgatt accggagaat gggggcgcct ggaactggct
      721 acaattacta tgcgctcact atcggggttg gcttatctga ttgtggttgg atcgctggtg
      781 ggtttcgttg catattcatg gctcatgcgc gttgccccca tgcccatcgt gtcaacctat
      841 gcctatgtca atcccttgat cgctgtcatc ctggggagtt tgctggctca agaagagctg
      901 actgtacgtg tgttactggc tgcaccaatc atcgtaagct ccgttgcgct cattaatatg
      961 gcgcgcttta agacaacatc tcgtgcgcct gcgcgtttgg cggtctatgc agcgtctggg
     1021 gaagattgag atgcatggat cttaaccccc agatccagat tatatgtctc gatgaggaat
     1081 aaaaacacat gatgagtacg gatcatataa aatcatttat ccgcgacctg gggattgacc
     1141 ttgttggagt ggcggatatg accacattaa acggtatgcc actcggtata tctgcagaag
     1201 gtgcatcctt cctagatgaa ttccctcgag cgattgtact gggggcacag tatggcaaga
     1261 ttggcaaaaa tgccaagggt cgggaagttt cctttttcct ggagaatgcc gctttacagg
     1321 tattggaata cttggaggaa gaaggaacac aagggttgat tatccataca gaggatgagt
     1381 acaacccgat cacgaggcag gggttgctct cattaaaagt gcttgcgaaa gccgccggac
     1441 ttggatggca ggggcgctct ttgctgatcg tttcccctga gtatggaccg gtgcaccgtt
     1501 ggatcgccgt tctaacagat ttggacattt cacccgatca aactctctcg aatgagtgtg
     1561 gatcatgttc cttgtgcgtg gataagtgcc cgcaagacgc ccttacactt gtgccatttg
     1621 aggatcaccc cgaaagccgg gaagctgtgc taaacgtccc ggcttgtaaa ggggataacg
     1681 gctgcaaggt gtgtattgtt gtctgcccgt ggttccgcca agctatttgc tgatggtaat
     1741 tttgcctttc atgccggcgt cgtagtgtac gccttcttgc tcgaaacaac ccatttccca
     1801 ttcaccgacc atctcgtcgg tcacggtgaa ggtgattgag gctgtatccc cttcttcagg
     1861 taatacaacc ataaatccag tatgcccctc atcgtgttca ccttccatgt gcatctcgcc
     1921 catcaccata ggttccgagc cgctctcttc aaacatatca tgctggtaac cgctcgggcg
     1981 gttgttcatc atcatcgcct cactcccaaa catgatttca tgtgcaagcg tacccttgtt
     2041 gaccaggtta atggtgactt gttgtccgac tttcaactcg agcgtgttgg gcgaaaaact
     2101 atattcgctc atttcgattg tataggtcac tggttctggt gtacttttag cgcaacccgt
     2161 cactaagatg gaaatcaatg ccacaaaagc caagaaaaat ttgccggttt tcatttttac
     2221 ctccaggttg taattggatt tatgcatttt aagactaagc gaggcttttc ctattttaac
     2281 tgacaacttc tgataatatt gaattatcgt gttgttgatg gagaaggctc cccatctgcc
     2341 tgttggcaaa taatatacca taatcggaaa agctcaggat tgtcatccac cagtttgccc
     2401 aaaaagggct tggtgctagc ctaaaaatcg aggaaacgat gaagcagttt aaaaaaaacc
     2461 ggatattcga gctcgccaaa cttggattac cacatatccc tgcagcgctg ggaaaattct
     2521 atcctttcct ggcaattatt tatgtattgc ttggtccagt gattacatct gcaatcgccg
     2581 ccataccgat tgcgatcgta ttcattattt cgggctccac acacgtcgat ttgcaggcga
     2641 tcatgggttc gagtgttggg atgacggggc tgatgctggt gggctttttg ccgatctacc
     2701 tcttggtttg gggctggttg cgcgttttcg aacatcgtcc actgtggacg atagggatgg
     2761 agagaaagga atggttgagt aaatatctgc gtggcgcgct ggtgggcagc gtgatgatta
     2821 gcgcggcggt gggcttgcct gctatgttcg gttacataca gctccaaggg aatagctctg
     2881 gagttagcgt tgctacgatg ggggcgctta ttctatttat aggttgggtg gttcaaggcg
     2941 cggccgaaga ggtgctgttt cgtgggtttc tgcttcagat tattggctcc cgctttggtg
     3001 tgatagctgg ggtgctggtt acctcgatct tgtttgccct actgcatatt ttcaatgcca
     3061 gtctcggcgt aatcgcgtta attaatctca tgctatttgg ggtatttgca gcgttctata
     3121 ccttgtggga ggggagcctt tggggagttt ttgcagtgca tagtttctgg aattgggtgc
     3181 aaggtaacct ttttggcatg gaagtgagtg gtctgccggt tcgcttggag gccttgatcg
     3241 acctaaaaga ggtaggacct gactggttta ctgggggagc gtttggacca gaagggggta
     3301 tcgcggtcac gctgttgttg ttggcgggga ttgccgtttt atggttgctt gaaagccgca
     3361 agcagagcga atcctgacat ttcggcattc gcgttgggtt gtattcttat tcaaaagatt
     3421 tcaaatcgtt caacctcaaa gacaaatatc gtcgcgccgc caacagttat gggtattggc
     3481 tctggcaggc cgggcgggaa gccttcaatc ggcgcggaga tgtattctac tcgcttgcgg
     3541 caacttaatt gtaaagccct gaccacgctc gcctcttgac ccttttctat cccaatcaat
     3601 aggatggcgt ttgtgcgcct aaaaaatcca ccagtgctgg ggaggcgcgt ggtggggatg
     3661 cccaatttgt tcaaggtgct cagtgcattc tcgacatctt gctcttgtac gatgacagcc
     3721 atcaaccggt tgacttgtgt atgctgagaa atattcccac tgagcaactc tggccaacct
     3781 gcggatggat tagtgatcgc cccgtcgatt tcagcggtga gttgattgaa aatctcgtct
     3841 gagatggtgc catctcgtcg taggctgttg attgcgctgc gctgtgcact cagatattcc
     3901 cggcgggcgg tatcaagctc ttcttgttct acttgtggat ctgaggacac gacctcttgt
     3961 acagaatctg ccagtgtgcg gttatgttct tgcagcagag gtccgatgat atcccaagaa
     4021 tggtctgaca gcaatccctg ggagtgatgg cggctcaagt gctcataagc atgccgggta
     4081 gcaactgcac gagcatggcg gcgctcatat tcatcctggg actcgctgcg ctcggttaac
     4141 ttaagctggg ttacaagagg tttcatggtg aagccttgga cgagaagggt gaaaagcacg
     4201 acaccaaagg ccatggcttg tagctgaggg ctggcttgcg gcaatgatgc tggcaggctg
     4261 agcgccagag ccaatgaaat tgccccgcgc aagccccccc aatagagcac gtgacgccac
     4321 ctgggtggta tctctttctc gaaccaggat agaccataaa ccccgatggc tcgggcgatg
     4381 aggatagcca cgatggccca aacgatcatt tgccagttct ggtacaggac aggcaaatgg
     4441 atttgtaacc caatcagcaa gaaaatgaat gagttagcca agaagcctgc atattcccag
     4501 aagttgaata ccacgatgcg cgtggtcggt gacatcccac gcgggccgat gttgccattg
     4561 atcaaaccgg ctgcaacgac tgccagcaca ccgctcactc caagtatttc tgcaatcaga
     4621 taggcgccat aagcgagcac gctggtgagg gttgtctcga ttaagtaatc atctatgcga
     4681 ctaatcattt gcgagattag cgctcccagc aacaagccaa ccacgaggcc tccacctgag
     4741 acgcgtacaa actcaaccac gctggaggca aggttgaagt gggaagcttc tgatgcggct
     4801 agaacgacta ccagatcaaa taccacgatg gcggttccgt cattaaacag gctctcgcct
     4861 tcaagaagca cctgcaggcg cttaggcacg cccatggtgc gaaacaggga taccacggat
     4921 accgggtctg tggccgctac cagagcacca aacaccatgg cgatgggtag tgccaagcct
     4981 gtgccccaag cgacgatgcc tcccacaata agcgtggtga taatgacacc cggaattgcc
     5041 agcgccagta tcggcgccag gttgcgccga agatcgttaa tgtttaaatg aaatgctgct
     5101 tcgaaaatca ggggaggcac cagcaaagcc aaaattaagt tcggcgcaat ctgaacatcg
     5161 acctgttccc atatcgtcat cgtcaggccc attagcacca gcccaacggt gtatggcacg
     5221 cgcaaacggt gggcaacaat gcccaccaat acagctacaa acagcagggc aatgacaatt
     5281 tcttcgatca ggatgatttg ttgatctata tccattgtga tttcttataa atatgggtag
     5341 tcacctgcca ctgaaattgc catacctagt tgggtactat gctgctactg gaataaagga
     5401 aatgccagtg tatataacac gctggcaaca aaacaggttg ctatcatcac tggaagaccg
     5461 cgtttgttcc aaagtggaga tgtgatggga ttgtgagttt tctctgataa tgtggcgacg
     5521 actatattgg ccgtggagcc gataattgta gcattgccgc caaatccagc cccaaaggcc
     5581 aaagcccacc agagtggtgt tacgtttata ccctgtagct caagatcctg aattacagga
     5641 atgagagcaa tggttatggg tacattgtcc accaacgccg agagaatagc gatgacccaa
     5701 ataagcagca caccgaacat tgccggattc actgtcctgc cccaagtcaa cacttcaacc
     5761 aggctgtgca acaagccaga tgattttagc ccgcccacca ttataaacaa tgcacagaaa
     5821 aaaatgagca cactccattc gattcgctta aacatctcag ccatgtcagg ttgcacccag
     5881 accactgcaa cagaagcggc tgctagggca atgaaagaag ggctagtgtg caagatttct
     5941 tccataaaga acaaaatcat cgtcgcgcat aatacaatga gtacttttcc cactgtaacc
     6001 cgatcggtaa gcgcttcaga agggttaagg tcaataattg cttttatgtt ggtgggtttt
     6061 ttggctagat ctgcccggaa caggtagcta attagcgcca atgcaaccag ccatgcaact
     6121 agcacaactg gtaacgaatg agttagaaaa tcattgaaag agaagtctgc agccgacgcg
     6181 attaagacgt ttggcgggtc accaaccagg gttgatacac ctccagtatt tgagagcaga
     6241 gcttcagcca tcaataaagg tgtggcgttg atgccgagaa tctcgcttat caggattgtg
     6301 accggggcaa tgagtacaac ggtggtgaca ttgtctaaaa ccatggagag caatgttgtg
     6361 agtgttccca gcgttattag caagcgcatc tgattgccac ccgaccaacg agtagcccag
     6421 atcgctaggt attgaaaaaa accagtgggt tccaggagcg caaccaggat catcattcct
     6481 agtagcaaac caaccgtatt gaaatcaatt gcctcaaccg cctgttcttc cgaatagaaa
     6541 ttaaatgcta gacccgcccc gatcatcact gctgcccctg ccatggaagt aatggtacgg
     6601 ttcagctttt cagaaaaaat taaataaagg ctcaggataa agatgattcc tgagagggtc
     6661 atgggtaagg tcatgtcata ccgccttggg atatgttcca cgttgagagc agggctaccc
     6721 cgatatcaac atgggatgcg atcccaacca actttccatc tgccgataca accaatatat
     6781 cggcaagatt ctggcgatac attattgcgg ctgccctcaa taacccacac gtttcatcga
     6841 cggatatggg atcaaggata atatctagca ctgattgcga cagagtagcg gttgaaggca
     6901 agcgggtttc gactgcgcca aagtcatgta caaatttaaa gtcctcaact aattggataa
     6961 aatcaggcat gaccaatttg actaaatctc tcaagcgtaa aacgccaacc agtactccgt
     7021 cctggtcaac cacaggtaag gtgccaatcc gcttttccaa tacgattgtc gcggcctctg
     7081 caatcgtagc agtctcagga atcgatacta cctgtggttt catacagtgc ttaatttcca
     7141 tggtctagct cctaatttat tgattgaaaa ggtagtgaat ggcctccatc caccaggatg
     7201 gtctgcccgc gaatcatgct ggcttgtggg ctgcatagga aggcgaccac attggctacg
     7261 tcttgggatg tcaccagcct gcctgcaggg atatgttcag tagctttatc gattagggac
     7321 tgttctattt ttacattgtc gaaatgcttt aaggcattcg tcaaaactat gccgggggag
     7381 actgcgttca cgacaatatt ttttggcgca agctccactg caaggtagcg cacgagcgcc
     7441 tccagagcgg ctttgctagc gccgacgacg atgtaatcgg gtagtacacg cgaagcgccg
     7501 ggactcgaga tagcaacgat cgacccgccg ccgcgttgct ccatcagcgg cactgctctt
     7561 tgcgctgata aaagcaaggc gtgagcgtta atgttcatgc tccaatccca gcctttgggt
     7621 ttttgttcca tcgctggacg gttgtatcca gaagccgcgt tgctgacaag gatatctagg
     7681 gtactgaatt ccttttcgac ggtttcgaag aggcgattta tttcatccac ttcgcccacg
     7741 tttgctttca cgacgaggga acggcgccca agggcacgaa tttcttcggc cgtctcctcg
     7801 gctgggcgac gatttcggaa aaaattgacg acgatgtcgg ctcctaaatt ggctaagtgt
     7861 agggcgatgg cgcgcccaat accgcgtccg gagccggtga tcagggcgat tttgccagta
     7921 aacatcaata ctattttact ggattatttg gagtttattg gcaatactga gacaattgct
     7981 ga
//
LOCUS       DBNGSX010000088         5443 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1391804, whole genome shotgun sequence.
ACCESSION   DBNGSX010000088 DBNGSX010000000
VERSION     DBNGSX010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5443)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1391804"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="AC16CB_02975"
     CDS             <1..501
                     /locus_tag="AC16CB_02975"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR3341461.1"
                     /translation="ILLVILGMRFAFGIAILAGLARFVPYVGPLTTTVVTFLVALFQG
                     ENYFGLLPYQYAILVVVASFILDQIYDNLISPRLLGEALGVHPAGVLIVAIIAANLIG
                     LIGLVLAAPVLATLRLISRYVGRKMFDLNPWPETEPPPPTSPLDVPWSRWIRRLRAWA
                     RSLKLK"
     gene            complement(599..1969)
                     /gene="dnaA"
                     /locus_tag="AC16CB_02980"
     CDS             complement(599..1969)
                     /gene="dnaA"
                     /locus_tag="AC16CB_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIR3341462.1"
                     /translation="MKAEQAWQATVGQLQLEMPKAAFNTWVRDTELLSYEDGSFVVGV
                     HNAYARDWLESRLSSTVKRLLVGMMNRSVEVRFIVWQNPKETTETSASPIVLGDAEEQ
                     IDTAPSPNGYHQSPSFVSRYSFDNFVVGHSNRLAHAACLAVSENPARAYNPLFLYGGA
                     GLGKTHLLHAIGSVNAARGLHVLYVTSEEFTNDLINALRTHTTQAFREKYRQPDVLLI
                     DDIQFIAGKESTQEEFFHTFNALHSQDKQLVMTSDRPPKAMVTLEERLRSRFEWGLIA
                     DIQPPDYETRLAILRSKADRAGYRMQSEILELIARRIQSNIRELEGALTRIAAFAELS
                     GMPMTAQLAESVLTDLLPRRNEVQPEEVIRKVADVFGVAFERMLGRDRSREVALPRQI
                     AMYLLREEANISLPQIGQTLGGRDHTTVMYGCDKVADLLEQDDRLRRQVIQIKEQLFG
                     MSQLGY"
     gene            2574..4478
                     /gene="dnaK"
                     /locus_tag="AC16CB_02985"
     CDS             2574..4478
                     /gene="dnaK"
                     /locus_tag="AC16CB_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR3341463.1"
                     /translation="MSKIIGIDLGTTNSVVAVMEGGEPFVISTAEGSRLMPSVVAFNK
                     NGERLVGQVAKRQAVVNPENTIYSIKRFMGRRYDEVETERKMVPYQVAQGPTGDARVH
                     IPQTKREYSPQEISAMILGKLKADAEAYLGESITKAVITVPAYFNDSQRQATKDAGKI
                     AGLEVLRIINEPTAAALAYGLDKKKNETILVFDLGGGTFDVSVLDVGEGVIEVRATNG
                     DTHLGGDDWDERIVNWIADEFKKEQGIDLRQDRQALQRLREAAEKAKIELSSVHETEI
                     NLPYVTADSGGPKHLQIKLTRAKFEQLTEELVNRLRGPFKAALNDANLSASEVNEVVL
                     VGGATRMPMVQELVRSLTNKEPHKGVNPDEVVAVGASIQAGVLGGEVKDVLLLDVTPL
                     SLGLETLGSVMTALIERNTTIPVKKSEVFSTAEDNQTAVDIHVLQGERPMAGDNMSLG
                     RFRLEGIPPAPRGIPQVEVTFDIDANGILNVTARDKATGKEQKVTITASTNLSKSDID
                     RMVNEASAHESEDRKRRELVEARNMGDSLTYQTEKTLRELGDKVPADERKNIEEKIKA
                     LREAIQGDDINRIKQLQEDLQNAFHALSQQLYAQQAQAGDTSPPDNGHDRGPSSDGEG
                     EVIEGEYREA"
     gene            4711..>5443
                     /locus_tag="AC16CB_02990"
     CDS             4711..>5443
                     /locus_tag="AC16CB_02990"
                     /inference="COORDINATES: protein motif:HMM:NF025104.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HIR3341464.1"
                     /translation="MQSTVPGVNTSWEAQRTVKISQASRNIPRHWQWRLLIMCLIIGD
                     ALMIGIGFRVAYYLLFETAISRSFLATPDGFYLYREFVFTTVPIWLAIFATNRLYSRQ
                     NLLGASKEYSLVFKATSTGMLLVFAVGFLLPGFNLHRGWLLLSWLLTFLFTTTGRLLL
                     YRAVVHLREHGYYLSPAVIVGANNEGLSLAKQLIRWRSSGIHILGFLDKKLPAGKKVI
                     NHLYCLGSVDELEAIVERYGVEEIIL"
BASE COUNT         1360 a         1424 c         1402 g         1257 t
ORIGIN      
        1 atcttgctgg tgatcctggg catgcgcttc gctttcggta tcgccatcct agctggtttg
       61 gcccgctttg tgccctacgt cggtccgcta accaccacgg tggtgacttt cctggtggca
      121 ttattccagg gcgagaacta tttcggcttg ctcccgtacc aatatgccat actggtcgtg
      181 gtagcatcat ttatcctcga ccagatctac gataacttga tctcgccgcg cttgctgggt
      241 gaagccctgg gggtgcaccc ggcaggtgta ttaattgttg ccatcatcgc tgccaacctg
      301 attgggctga ttgggttggt gctggcagcg cccgtcctcg ctaccctgcg ccttataagc
      361 cgatatgtgg gccgaaaaat gtttgatctg aatccttggc ctgagacgga gcctccgccc
      421 ccaaccagcc ctctggatgt gccctggtcg cgctggataa ggcgcctgcg cgcctgggca
      481 cgctcactga aattgaaata aatagaccct gggtagggaa ggcaaactac ccaggatcca
      541 tttttaatca tctagctctt tgggtaagcc aacacgtacc ccaatgagct agtcggtttt
      601 aatatcccaa ttgcgacatg ccgaataact gctccttgat ctggatcacc tgcctgcgca
      661 gacgatcatc ctgttccagc aagtcggcta ccttgtcaca gccgtacatg acggtcgtat
      721 ggtcgcgccc acccagggtt tggccaatct gaggtaagga gatattggcc tcctcgcgta
      781 aaaggtacat ggcaatctgg cgtggtaagg ccacctcgcg agagcgatcg cgccccagca
      841 tccgctcaaa ggccacaccg aatacgtcag ctactttgcg tattacctct tcaggctgca
      901 cttcgttacg gcgtggcagg aggtctgtca gcaccgattc ggcgagttgc gctgtcatgg
      961 gcatgccgct cagctcagca aaggcagcga ttcgggtaag agccccctcc agctcgcgga
     1021 tattcgactg aatgcgacga gcgataagct ccaatatttc gctctgcatg cggtatcccg
     1081 ctcgatccgc cttcgagcgc aaaatagcca aacgggtttc gtaatcaggc ggctggatat
     1141 cagcgatcag tccccactcg aagcgtgagc gcaggcgctc ttccaaggtc accatcgctt
     1201 tcggaggccg gtccgaggtc atgaccagtt gtttgtcctg gctgtgcaag gcattgaagg
     1261 tatgaaagaa ctcctcctgg gtcgattcct tgcctgcaat gaactggata tcatcgatca
     1321 gcagtacatc gggttggcga tacttctctc gaaatgcctg agtcgtatgg gtacggaggg
     1381 cgttgatcag gtcgttggtg aactcttcgg aggtgacgta caacacgtgc aagccacgcg
     1441 ccgcatttac actaccaatg gcatgcaaca agtgtgtctt gcccaagcca gcgcctccat
     1501 atagaaacaa tggattatag gcacgggccg ggttctcgga taccgccaag caggcggcgt
     1561 gggccagccg attgctatgc cctactacaa aattatcaaa tgaatagcgg gagacgaagg
     1621 agggagattg atgataaccg tttggcgagg gagcagtatc gatctgctct tctgcatctc
     1681 ctaacactat cggcgatgca gatgtctctg tcgtttcttt cggattctgc cagacgataa
     1741 atcgcacctc aacactgcga ttcatcatgc caaccagcag ccgtttcacc gtgctggaaa
     1801 ggcggctttc cagccagtcg cgagcatagg cattgtggac ccccactaca aaactgccat
     1861 cctcataaga gagcaattct gtgtcgcgca cccatgtatt gaaggcagct ttgggcattt
     1921 ccaattgtaa ttgaccaact gtggcttgcc aggcctgttc tgctttcatc ttgccttcct
     1981 cctcatccag aaatttgcaa catttaagcc gcccctgcag agaagaccag caggggaaaa
     2041 taaaggcttt caattgttac gaggtttatt accaccaaaa ctcagaagga ctgaccccca
     2101 aacacctcac atgtccatca tcctgtacat tctagcagat acctgccctg atatgcaata
     2161 ctaaaaccct acggtaacct aaaaaaaaac aaaactttaa ggttcgccaa tgttaaaaac
     2221 aacctgatat tttgcactta aatatgatat tgcaaaacat tatcagaagg ctatagatag
     2281 gggataccct cttgataacc cccacatcta cccctatgca gggcttccta ttcccccccg
     2341 caggtgcttg ctatagaatc cttaaaatat tacgggcaca gaaattgcac cctccttgct
     2401 tggtaaaagc cagttatcgc gcttcgcaaa ataacgtccc aaaaacgttg atatgcctct
     2461 tacacactgt taacggattt cttgcacaag tgcgttatcc ttacctacac aagtgcgtta
     2521 tccttaccta tgaatataaa ctgacgaaaa ttaatcagat agaggagaga gttatgtcga
     2581 agattatcgg tattgatctg ggaacaacca acagcgtggt agccgtgatg gaaggcgggg
     2641 agccatttgt tatatctacc gctgagggct cccggctcat gccttcggtt gtggctttca
     2701 acaagaacgg ggagcgcctg gttggacagg ttgccaagcg ccaggctgtg gtaaacccag
     2761 aaaatacaat atattctatt aagcgcttta tggggcggcg atatgatgaa gttgaaacag
     2821 aacgaaaaat ggtgccgtat caggtcgcac aaggtcccac aggagatgct cgggtgcata
     2881 tcccgcagac caaacgcgag tatagtcccc aagagatctc cgcgatgatc ctgggaaagc
     2941 tcaaagcaga cgcagaggct tacctgggcg agtcaattac gaaggcggtc atcaccgtgc
     3001 ctgcatattt caacgacagc cagcgccagg ctaccaaaga tgctggcaaa atcgctgggc
     3061 tggaagttct acgcatcatc aacgagccca ccgctgcagc cctggcttat gggctggaca
     3121 aaaagaaaaa cgagacgatt ctcgtctttg acctgggtgg cggcaccttt gatgtcagcg
     3181 tcctggacgt gggtgagggc gtgatcgagg tccgtgccac caatggcgat acacacctgg
     3241 gtggcgacga ctgggatgag cgcattgtca attggatagc ggatgaattc aaaaaagaac
     3301 agggtattga cctgcgacag gaccgccaag ccttacagcg cctgcgcgag gctgctgaaa
     3361 aagccaagat tgagctctct agcgtgcacg agacggagat caacttaccc tatgtcacag
     3421 ccgactcggg cgggccgaag cacttgcaga ttaagctcac ccgcgccaaa tttgaacagc
     3481 tgacagaaga attggtcaat cgcctgcgcg gtcccttcaa ggcagctttg aatgatgcca
     3541 acctgagtgc cagcgaggtt aacgaagtcg tgctggtagg tggtgctacg cgcatgccaa
     3601 tggttcagga attggtgcga tcgctcacca acaaagaacc acacaaaggt gtcaaccccg
     3661 acgaggtggt cgcagtcggt gcttctatcc aggccggcgt gctgggcggc gaggtgaaag
     3721 acgtactgct gctggatgtc actcccctct ctttgggatt ggaaaccctg ggaagcgtaa
     3781 tgaccgcgct tatcgagcgc aataccacca tcccggtcaa gaaaagcgag gttttctcta
     3841 ctgcagagga taaccagacc gccgtagata ttcacgtgct gcaaggcgag cggcctatgg
     3901 ctggtgataa tatgagcctt gggcgttttc gcttggaggg catcccaccc gcgccgcgcg
     3961 gcattcccca agtcgaggtc accttcgata ttgatgccaa tggcatcctc aacgttaccg
     4021 cccgcgataa agctactggt aaagagcaaa aggttaccat caccgcttcg acaaacctat
     4081 ctaagagcga tattgaccgc atggtgaacg aggcgagcgc tcatgaaagt gaagaccgca
     4141 agcgccgcga gctggtagaa gctcgtaaca tgggtgatag cctaacatac cagacagaga
     4201 aaacgctgcg cgagttgggc gataaggtcc cagcggacga gcgcaaaaat attgaagaga
     4261 aaatcaaagc gctgcgtgag gccatccaag gtgacgatat caaccgcatt aaacagttgc
     4321 aggaagattt gcaaaacgca ttccacgccc tgagccagca gttgtacgcc caacaggcgc
     4381 aggcaggcga cacctcgccg cccgataacg ggcatgaccg aggtcccagc tcagatgggg
     4441 aaggcgaagt gattgagggc gaataccgag aagcttaatc gataacaagt ggttgtaaaa
     4501 gattaagcag ggatttgtgg gcagggttgc acccacaaat cccttttcta tttaaattaa
     4561 ataaaggtaa catttcgcat aatatttcgt taaccatatt tagagagtct ccgcccggaa
     4621 aagacacctc ctctcaggag gaattcttgg gggaagccgc aaggaaaaac caatctctca
     4681 ttgtccgcat cgattttgga ggagaggggt atgcagtcaa ctgtgccagg tgtgaataca
     4741 tcctgggaag cccaacgtac tgttaaaatt agccaggcat ccagaaatat cccgcgccac
     4801 tggcagtggc gtctgttaat catgtgccta atcataggcg atgcgctcat gatcgggatt
     4861 ggttttcgcg tggcttacta cctgctcttt gaaacagcta tctcgcgatc gttcctggca
     4921 acgccagacg gtttttattt atatcgggaa tttgtgttta ccaccgtccc catctggttg
     4981 gcgatatttg ccacaaatag actttatagc aggcagaacc ttttgggtgc ttccaaagag
     5041 tattccttag ttttcaaagc tacaagcact gggatgctgc tggtattcgc tgtgggtttc
     5101 cttttacctg ggttcaacct ccatcgcggc tggctgctgc tgtcctggtt acttacattt
     5161 ctattcacca caacaggtcg cttgctcctt tatcgcgccg ttgttcatct gcgcgaacac
     5221 ggatactatc tctccccggc agtgatcgta ggagcgaaca atgaagggct ctccctggcc
     5281 aaacagctca tccgctggcg ctcgtcggga atccacatat tgggttttct ggacaaaaaa
     5341 ttgccagccg gtaaaaaggt gataaaccac ctctactgtc tgggatcagt ggatgagctc
     5401 gaagcaattg tcgaacgata tggtgtagaa gagattatcc tag
//
LOCUS       DBNGSX010000089         4303 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_6782, whole genome shotgun sequence.
ACCESSION   DBNGSX010000089 DBNGSX010000000
VERSION     DBNGSX010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_6782"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="AC16CB_02995"
     CDS             complement(<1..312)
                     /locus_tag="AC16CB_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341465.1"
                     /translation="MVMKNAIQNHLPHTFKPNSGVGFALFLLSTATLAFEINLTRLFS
                     VAQFYHFAFMVVSLALLGFGASGTFLAIFPLKAKQSIDRVMGNLALGASVTMLASFLL
                     SN"
     gene            495..1646
                     /locus_tag="AC16CB_03000"
     CDS             495..1646
                     /locus_tag="AC16CB_03000"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3341466.1"
                     /translation="MSFYLALKEIWRLRGRYLLFSMVIALITILVLFIAALAGGLANA
                     NKQLIEKLDGEMLIYQNNVDLQLTASRLGQSKLSDIRRIDGVADVGPVGLATATVVFA
                     DGRSPIDIALVGVEPGKPGDPPLMAGPGLRTKRNNDAVIDEDLASRAGLTIGDRFIIK
                     SVQGTEEEFYELSVVGITDSRQYFFLPSAIMPIRTWSKVRPQGDGESSTSEIIFNLAA
                     VKLQDPQQFDLVAQKLEYFVDGIEVADKQAAILALPGYTAQQSTLNTQQIFTLLIGVL
                     VVGGFFQIQTLQKVAQIGVLKAIGSSSATVAFATLLQIILVTLIGVGIGSLGTMLLVL
                     GMPSEIPILFTGSTVISAAVLLLFIGPIGGLVSIRLALKIDPLTAIGLS"
     gene            1697..2494
                     /locus_tag="AC16CB_03005"
     CDS             1697..2494
                     /locus_tag="AC16CB_03005"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3341467.1"
                     /translation="MTIALRTDEITKIYGKDTSAVVAVDQVSLEVKRGEFIALVGPSG
                     SGKTSMLAMLAALLSPTSGSIFIGDVALAEMKEKERTRFRREKIGFTFQTNNLVPYLT
                     ALENVELMLRLNKTLNKEGKAHAKELLARLGLQERLNSLPNQLSGGQQQRIAIARALV
                     HRPEVVLADEPTANLDTERAFQVVETFANLIHEQNRAGIMVTHDLRMTKYVDRVIQMV
                     DGKVTRSLDDRAAIDLLAQTSSFEAAEQYIGSTPFQYAVAPAASKVA"
     gene            2614..3669
                     /locus_tag="AC16CB_03010"
     CDS             2614..3669
                     /locus_tag="AC16CB_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR3341468.1"
                     /translation="MTNHRITSQDVAEKAGVSRTTVSLVLNNVPGVKISPQTRRRVLD
                     AARELNYVPEAAAQALASRRARIIGLILIRNPHQISTDAFLTQILDGLIETIRPSGMR
                     LLIDIVEPQHQKETYLQLIQAKHIDGIILSGPRYDDDALSSLLEVNFPAVLLGQIPDS
                     DFAFVDVDNRAAVNTAVAHLINLGHSRIACITNAPISYTAALDRLTGYKQALKENGIA
                     LDSELIRYGDFDIESGYRQMHSLLASNVTMTAVFIGSDTLAYGAVAAIRERGLRIPQD
                     IAVVGFDDIPFSRYSDPPLTTVRLPAGDLARRSAEILSQLLAGDEPESKQVILETQLV
                     VRDSCGASRSNRRMKLQ"
     gene            3785..>4303
                     /locus_tag="AC16CB_03015"
     CDS             3785..>4303
                     /locus_tag="AC16CB_03015"
                     /inference="COORDINATES: protein motif:HMM:NF013697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR3341469.1"
                     /translation="MNRKAISTILSVFFILSFVLAACAPKATPTEPPVAETAAPVVTE
                     APPAMPEADFAMMPGGFLERALTGEFSGTTVTVDGAFEGNDPDGVKMDRSVAQFVELT
                     GINVNYIGNKEFEATISVRVEAGDAPDIADFPQPGKMANFVRDGKVVDVTSWMTEEWL
                     SQQYNQSWRDMAT"
BASE COUNT         1114 a          994 c         1130 g         1065 t
ORIGIN      
        1 attcgagagg agaaatgaag cgagcatggt tacggatgcg cccagggcaa gattgcccat
       61 cactcgatct atgctttgtt tagccttcag cggaaaaatt gccaagaaag tgccactggc
      121 accaaagccc aacaatgcca ggctaacgac catgaaagcg aaatggtaga attgggcgac
      181 cgagaaaagg cgtgtcagat tgatttcgaa agccagagtg gctgtggata acaaaaagag
      241 ggcaaaaccg accccagaat ttggtttaaa ggtgtgaggg agatgatttt gtattgcatt
      301 tttcatgacc atgtacgtct cccaaaatta ttatactgct tggcgggaga gataatgtat
      361 aaggtaaata gggaaaatgc tgttagaaaa gagaatcttt tacaaaacca ttgacaaaac
      421 tgtacaatat gtgtacaatg taaatgaacg taattaaatc taatttggta taattacgta
      481 gcatcggagg gagaatgtcc ttttacctag cattgaaaga aatttggcgc ttgcgtgggc
      541 gatacttatt gttcagcatg gttatcgcac tgattaccat tctggtgttg ttcatcgctg
      601 cgctggctgg ggggctggcg aatgctaata aacagctcat cgaaaagctg gatggcgaga
      661 tgctgatcta ccaaaataat gtcgacctac aactgaccgc cagtcgcttg ggacaatcta
      721 agctgagcga tatccgccgc attgacggcg ttgcagatgt cggccctgtc ggtctcgcta
      781 cggcgacggt ggtttttgcc gacgggcgat ccccgattga catcgccttg gtgggggtag
      841 aaccaggcaa gcctggcgat ccgcccttga tggctggccc gggtttgcgc acaaagcgaa
      901 ataatgatgc ggtcatcgat gaagacctgg cttcacgggc gggcttgacc atcggagacc
      961 ggtttattat caagagtgtt caaggcacag aggaagaatt ctatgagctg agcgtggtgg
     1021 gtattaccga ctctcgccag tattttttcc tgccatcagc aatcatgccc attcgaacct
     1081 ggtcaaaggt gcggcctcaa ggggacggtg aaagctccac atccgagatt attttcaatt
     1141 tggccgcggt gaaacttcaa gatccacagc agtttgatct ggtcgctcaa aagttggagt
     1201 attttgtgga tgggattgag gtggccgata agcaggctgc cattctagca ctgccaggat
     1261 atacagctca gcagagcacg ctgaatacgc agcaaatctt caccttgttg atcggcgtat
     1321 tggtagtcgg cggtttcttc cagattcaaa cgctacaaaa ggtagcgcaa attggtgtgc
     1381 taaaagcaat tggcagctcg agcgctacag tggctttcgc aaccttgttg cagatcatcc
     1441 tggttacttt gattggtgtg ggcatcggca gcctgggtac gatgctgctg gtgctgggca
     1501 tgccgagcga aattcctatc ctctttaccg ggtcgacagt gatttcagca gccgtactgt
     1561 tgctcttcat tggcccaatt ggaggtctgg tatccattcg cctggcactg aaaatagacc
     1621 cgctgacagc cattggactg tcttagtaat tgaaactaca gcgctctctg acttcaaata
     1681 acgggaagaa atatttatga ccatagctct aagaacggat gagataacga aaatatacgg
     1741 taaagacacc tcggcagttg tggctgtgga tcaggtctca cttgaggtga aacgcggtga
     1801 attcattgca ctggttgggc ccagtgggtc tggaaagact tcgatgcttg cgatgctggc
     1861 tgctttgctc tctccaactt caggaagtat tttcatcggg gatgtggcgt tggcagagat
     1921 gaaagagaaa gaacgcacaa gatttcgccg ggaaaaaatc ggttttactt tccagactaa
     1981 taaccttgtg ccctacctga ctgctttaga gaatgtggaa ttgatgctgc gtctcaataa
     2041 aaccctgaat aaagaaggaa aagcccacgc caaggaatta ttggctcgat tgggcttgca
     2101 ggagcgatta aatagcctgc caaaccagct atctggaggg caacaacagc gcatagccat
     2161 cgcccgcgcc ttggtacatc gccctgaagt tgtgctggct gacgagccta cagccaattt
     2221 agatacagaa cgggcatttc aggtggtaga gacatttgca aacctgatcc atgaacaaaa
     2281 ccgggcaggg atcatggtga cccacgatct tcgcatgacc aagtatgtgg acagggtcat
     2341 tcaaatggtg gatggaaagg tgacacgttc tctggatgat cgggcagcta tcgatctgct
     2401 ggcacagacc agtagttttg aggctgcgga acagtatatc ggtagcactc catttcaata
     2461 tgccgtagcc ccggctgcgt cgaaagtggc atgacaaact agcacccgtt tggtgaatct
     2521 acaataattg tattgactat tatctaatca gaatatataa ttaacgcgcg ttactaacgc
     2581 gtgttagtta cagatacatt cccaggattt cgattgacca accatcgtat aaccagtcag
     2641 gatgttgccg aaaaagccgg tgtttcgcgg accacggttt cgttggtgtt aaataacgtc
     2701 ccgggcgtga aaatcagtcc ccaaacccgc cgacgagttc tggatgcggc acgtgagctt
     2761 aactatgtcc ccgaagcagc tgcccaggcc ttagccagcc ggcgtgctcg cattatcggc
     2821 ttaatcctga ttcgcaatcc ccatcaaatc tcaactgatg ccttcttaac ccaaatttta
     2881 gatgggttga tagagaccat ccgcccatcg ggcatgcgtt tgctcatcga tatcgtcgag
     2941 cctcagcacc agaaagaaac ttatttacag cttattcaag ccaaacacat tgatgggatc
     3001 atcttatccg gcccgcgtta tgatgatgat gccttaagta gcttgctgga ggttaatttt
     3061 ccggctgttt tgctgggaca gattcccgat agcgactttg catttgttga cgtggataac
     3121 cgcgccgcgg ttaatacagc cgtggcgcat ttaattaacc tgggtcattc tcgaattgct
     3181 tgcattacca atgctcccat ttcatatacg gccgccttag accgtttaac gggctataaa
     3241 caggccctta aagagaacgg gatagccctt gattccgaat tgatccgcta cggagacttt
     3301 gacatcgaga gtgggtatcg acaaatgcac agcctattgg cgagtaatgt tacaatgact
     3361 gcagttttta tcggtagtga taccttggcg tatggcgcgg tggctgctat tcgcgagcga
     3421 ggactgcgta ttccacagga tatcgctgtg gttggattcg acgacatccc cttttcccgc
     3481 tactctgatc ccccactgac caccgtccgt cttccggcag gtgatttggc acgccgttca
     3541 gcagaaatat tatcccagtt gttagccgga gatgaaccag agagcaagca ggtcatcctg
     3601 gaaacccaat tagtagtgcg tgattcgtgt ggtgcatcac gtagtaatag acggatgaaa
     3661 cttcaataat cagttcagcc ttcattacgt ctcggagtat gaaaggaggt ggttctcatt
     3721 agataaaatc agttcggcca aaattcggca aagacaattc aatctataca ttaaggagta
     3781 gaagatgaac cggaaagcaa tttccacaat actatctgta ttttttatcc tgtcgtttgt
     3841 gctggcggcc tgtgctccaa aagcgactcc aacagaacca ccggttgcgg agacagcagc
     3901 cccagtggtg acagaagctc ctccggcgat gcctgaggca gatttcgcga tgatgccggg
     3961 tggttttctg gagcgagcct tgaccggcga gttttcaggg acgacggtga cggtggacgg
     4021 cgcattcgaa ggcaacgacc ccgacggcgt caagatggac cgctcagtag ctcagttcgt
     4081 agaactgacc ggcattaacg tcaactacat tggcaacaaa gaattcgagg ccacgatctc
     4141 tgttcgcgtc gaagccggcg atgcgcccga cattgcagac ttcccgcagc cgggcaagat
     4201 ggctaacttc gtgcgggatg gtaaggtggt tgatgtcacc tcctggatga ccgaagagtg
     4261 gttgagccag cagtacaacc agagctggcg ggatatggcc act
//
LOCUS       DBNGSX010000090         3095 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_630323, whole genome shotgun sequence.
ACCESSION   DBNGSX010000090 DBNGSX010000000
VERSION     DBNGSX010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_630323"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..325)
                     /locus_tag="AC16CB_03020"
     CDS             complement(35..325)
                     /locus_tag="AC16CB_03020"
                     /inference="COORDINATES: protein motif:HMM:NF042802.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6773 family protein"
                     /protein_id="HIR3341470.1"
                     /translation="MIMIRDERIGKAKNQIAASGYGIWYILLLISLLYRQFILDQPVT
                     EYWDIALIFFIGTFYVAVASFAKGAVYESTITRFGKRAIPIIVITIVLVNYQ"
     gene            complement(415..867)
                     /locus_tag="AC16CB_03025"
     CDS             complement(415..867)
                     /locus_tag="AC16CB_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341471.1"
                     /translation="MSKKPINIDEREMNIFRNICTTLYIITLFSLIGMQLYRQFALHQ
                     SQEEWNDIALLIAFNVVVLLGSVLYVSGTINPKKIKFRYLIAGYLGFILFGFLFTIFK
                     YTVLLGQNIGLTQVLDYLLIVASISGLLALTWGLLAFLGSKRIEKLIE"
     gene            complement(860..1081)
                     /locus_tag="AC16CB_03030"
     CDS             complement(860..1081)
                     /locus_tag="AC16CB_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3341472.1"
                     /translation="MTMKAQVNMKLKLARVEKQLSQQELADIVEVTRQTIGLIEKGNY
                     NPTLKLCVKIAKALDRTLDQLFWEDEKGE"
     gene            complement(1457..>3095)
                     /locus_tag="AC16CB_03035"
     CDS             complement(1457..>3095)
                     /locus_tag="AC16CB_03035"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3341473.1"
                     /translation="VTWAAGFGYADLEKQVEATPSTAYPLASLTKPFAAAIIMQLVED
                     GTLSLDDPITDYGVDFSSDGVIELRHVLSHTSHGMPGEEYRYNGDRFADLGYLVRNAS
                     GRSFRELLNARILEPLEMTNTAPNPISMGDDFLDVFRIWMDADNTRVYRATAKPYRLD
                     QEYKLVKSGCPDFFTPAAGLISTVLDIAKFDIAMDQNILLEQETKVQMFTPTTSNNGS
                     ELPYGIGWFTQEYKDQPLIWHYGWQPSCGSSLILKIPDEEITFIVLANSDNLSRPYRL
                     GSGEVLAIDSILALAFYKTFVFEPQYSLVVPQIDWEADESDLVAQLQEVENENVNEIL
                     QRELLSYRKLFHSVGRVDQAEKLRNVYEKVFMNNEARLENYAELLELGELPWIYLPLP
                     RMRHVTILGFFVLIVLSVFTLWPIDYAIGRLRTRKSGLSRSVDKNKKAARNARAAAYL
                     AVITCLVVVFLFVSYISSYPNGGLLEWKNGTMLVKTLIGFANLSVVFSFLLAGFTIQA
                     WRKGYWSAGWRIHYTLTTIAILCNSYLWQQLDLLGWV"
BASE COUNT          898 a          729 c          616 g          852 t
ORIGIN      
        1 agacccattt atctttatgc ccggtgacaa caaactactg gtagtttact aacacaatcg
       61 ttataacaat aatcgggata gcacgtttgc caaatcttgt tatcgtactc tcatataccg
      121 ctcccttcgc aaaactggcg actgccacat agaatgtgcc aataaaaaaa atcagggcaa
      181 tgtcccagta ttcagttact ggctgatcca aaataaactg cctatacaac aacgagatca
      241 gcaacaagat gtaccatatc ccgtatccac ttgccgcgat ttgatttttg gccttaccaa
      301 ttctttcatc tcgaatcatg atcataccct acatcccaaa atttctaatc aagcgtattt
      361 ttcgttgttc tgtcgatcga caactcctga ctacaaacca tatgagatat attttcactc
      421 tatcagcttt tcaattcttt tgcttccgag aaacgcgagg agaccccacg taagcgcaag
      481 gagcccacta atagacgcga ctatgagtaa atagtccaat acttgcgtca ggccaatgtt
      541 ctgccccagt agaacagtgt atttaaagat cgtaaacagg aaaccaaata ggataaagcc
      601 gagataaccg gcgatgagat agcgaaattt gattttcttc gggttgatgg ttccactaac
      661 atacaaaact gaccccaaca aaacgacaac attaaatgca atcagaagag cgatatcgtt
      721 ccattcttct tgtgattgat gcagggcaaa ttgtctgtac aattgcatgc caatcaacga
      781 aaacaaagtg ataatatata acgtggtaca gatattccga aagatgttca tttcgcgttc
      841 atcaatattg atgggtttct tactcacctt tttcatcctc ccaaaacagt tggtctagcg
      901 ttctatccaa tgcttttgca attttgacac aaagtttgag cgtagggtta tagtttccct
      961 tttcaatgag cccgatggtt tgacgggtca cctcgacgat gtcagcaagt tcttgctggg
     1021 agagttgttt ttcaactctg gccagtttga gcttcatgtt aacttgtgcc ttcatggtca
     1081 taatatacta tatcaattgc ataatgtcaa gtataaattg cattaagcaa tttaattatt
     1141 acaatttgtg aagtagtttc acgcttatac gcttccccgt tctgcgcgga aaagtactcg
     1201 aataagagat agggtagata tctcacgagt ggctgaaaaa tgaaggattg gcagtgaaat
     1261 ttcctcgggc aataaatgca tcatttgatg tacgtcaggc acatatactc ccaaccaccc
     1321 gggtcatcca cctcaggcta gcgccccaac aggctcagat agattgaact cgatcatatg
     1381 agatggttgc tcattaggtt gtacactcct gggatccatg cgaattctcc ttgtccattc
     1441 catccaaagc cttgaattag acccatccca acaaatctaa ttgttgccat aaataactgt
     1501 tgcatagtat agctatggtt gtgagcgtat agtggattcg ccatccggcg ctccaatagc
     1561 cttttctcca cgcctggatt gtgaaacccg ccagcaagaa cgaaaaaaca acgcttaaat
     1621 tcgcgaaccc gatcagtgtt ttaacaagca ttgtcccatt cttccactcc aataaccctc
     1681 catttggata gctggaaatg tagctgacaa agagaaatac gacaactagg catgtgatta
     1741 cagcaagata tgccgcagct cgagcgtttc tcgctgcttt tttatttttg tcaactgatc
     1801 tggataagcc agactttcga gttcgtaatc gaccaattgc ataatctata ggccataggg
     1861 tgaacaccga gagaacaatc agcacgaaga aaccaagaat ggtgacgtga cgcattctcg
     1921 gcaatggaag atatatccag ggtaactcac caagctcaag taattcagcg tagttttcca
     1981 ggcgcgcctc gttgttcata aatacctttt cataaacatt ccgtaacttt tcagcttgat
     2041 ccactcgacc aacgctgtga aaaagtttcc gataggataa tagctccctc tggagaattt
     2101 cattcacatt ttcattttca acttcctgca attgggctac gagatcactc tcatcagcct
     2161 cccaatctat ttgaggcacc accaggctat attggggttc gaagacgaat gtcttataga
     2221 acgctaatgc caggatcgaa tcgattgcca atacttcccc cgaccccaaa cgatatgggc
     2281 ggctcaagtt atctgagttt gccagaacga taaaggttat ctcttcatca ggtattttaa
     2341 ggatcaaaga tgatccacaa gaaggctgcc agccgtaatg ccagattaag ggttgatctt
     2401 tgtattcttg tgtaaaccag ccgataccat agggcaactc gcttccgttg tttgatgtgg
     2461 ttggggtgaa catctgcacc ttggtttcct gctcgagcag gatattttga tccatggcga
     2521 tatcaaattt agctatatca aggacagtcg aaatgagacc agcagctggc gtgaagaaat
     2581 cggggcaacc gcttttcact agcttgtatt cttgatccaa tcgatagggc ttggccgttg
     2641 ctcgatatac cctggtgttg tctgcatcca tccaaatacg gaacacatcc agaaaatcat
     2701 cacccatgct gatgggattg ggagcagtgt tggtcatttc gagaggctcc agaatccgag
     2761 catttaacaa ctccctaaac gaccttccag aagcattcct aaccaaataa cctagatcag
     2821 caaaacgatc tccattgtag cgatattctt cacccggcat tccgtgggag gtatgcgaca
     2881 agacatgcct caactcgata acgccatcac tggaaaaatc caccccatag tcagttatcg
     2941 ggtcatcaag actaagcgtt ccgtcttcaa cgagctgcat gataatcgct gcagcaaaag
     3001 gttttgtaag agatgctagc ggataagcag tgctgggggt cgcttcaacc tgtttctcga
     3061 gatcagcata gccaaatcct gctgcccaag taact
//
LOCUS       DBNGSX010000091         2668 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_630362, whole genome shotgun sequence.
ACCESSION   DBNGSX010000091 DBNGSX010000000
VERSION     DBNGSX010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_630362"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1581
                     /locus_tag="AC16CB_03040"
     CDS             <1..1581
                     /locus_tag="AC16CB_03040"
                     /EC_number="3.4.22.70"
                     /inference="COORDINATES: protein motif:HMM:NF016119.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HIR3341474.1"
                     /translation="DAGAAYVYIHNGKTWVLQDELTAKDASPGDTFGVSVAISGNTIV
                     VGATGVNLEEQDEEDEELKDVGAAYVFTRSGGDWVQQVKLTASDREEDDSFGSAVAIH
                     KNTVAVAAETKTFNTGSVYVFYRSGVKDWKQQAKLATFPPSFNFGSSIDIYGDLLAVG
                     STQFNPLEDSGTGGVFLYRREGKVWEKETELEAEKGRIGDAFGNSVAIYGNTVVVGAH
                     HADPDLGAGRVTSAGTAYVFTRSAGAWKEEATLLASNSGAFDQFGQSVDIYGSTIVVG
                     ADGATQAGNSGAGAVYVFTKKNGTWNLATRAVTDPTSDDDAFGKSVGVYRDWFIIGAS
                     GRDPNSKTRAGEAFLSLLGSVQLPNTGFAPGQQTILPEQPSSLAYEDYGEMWLEIPSL
                     QQKMTIQGVPKGDNGWDVRWLSRQAGYLEGTAFPTWSGNTGLAGHTVLPDGTPGPFAK
                     LHTLSWGDQVYIHAWGKRYIYEVRENSMVQPNTLKVLKHEELDWVTLITCQGYDEKSG
                     KYRWRRVVRAVLVAVVEE"
     gene            1609..2505
                     /locus_tag="AC16CB_03045"
     CDS             1609..2505
                     /locus_tag="AC16CB_03045"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR3341475.1"
                     /translation="MKKQLAFVLGGGGARGALQVGALRALMEAGYQPDLLVGTSAGAI
                     NATFMAVRGMSLQSITELVQAWHDAATAEFFPQNYLWLTVRALFRRPDVHTSHRLRDF
                     FVSHGLGPELRFGDIQGIRLVLVATDLNASQLALYGLDPAEYVLEGLLASTALPPWVT
                     PIERDGSLLMDGGAISTLPIEPAMLTGATEIIALDLYDPNSLPHEPNGFGPFLAKLLN
                     TVERRQTEMELALASARRVPVHYLHLRAPDSVPLWDFSHAGELIEIGYELARAQIPRW
                     QAESKPRFLKGLQGFIEGTIGP"
BASE COUNT          600 a          733 c          796 g          539 t
ORIGIN      
        1 gatgccgggg cagcatatgt ctacatccac aatggtaaga cttgggtact gcaggatgaa
       61 ctcacagcca aagacgcctc ccctggggat accttcggcg tttcggttgc gatcagcggt
      121 aataccatcg tcgtgggggc taccggtgta aatctggaag agcaggatga agaggatgag
      181 gagctgaagg acgttggagc tgcctacgtg ttcacccgca gcggcggtga ttgggtgcag
      241 caggtcaagc taaccgcctc ggaccgtgaa gaggatgact cttttggctc ggctgtggcg
      301 atccataaga acactgtcgc ggtcgccgct gaaaccaaga catttaatac cggctcggtg
      361 tatgttttct acaggagcgg cgtcaaggat tggaaacaac aggccaagct ggcaacattc
      421 ccaccgagtt tcaattttgg ttcttccata gacatctacg gagatttgct agccgttggc
      481 tcgacccagt tcaaccctct ggaggatagc ggcacaggcg gggtcttcct ttaccggcgg
      541 gaagggaaag tctgggagaa ggaaacagag ctggaggcag aaaaaggccg cattggtgat
      601 gcctttggca actcggtggc catatacggc aatacagtcg ttgtgggcgc gcaccacgct
      661 gacccagacc tgggcgcggg gcgggttacc agcgctggca cagcttacgt attcacccgc
      721 agtgcgggcg cctggaaaga ggaggccacg ctattggcga gcaatagtgg cgccttcgac
      781 cagtttggac agtcggttga tatctatggc agcaccatcg tggtgggagc agatggggct
      841 acccaagcag gcaactcagg tgcaggagca gtgtacgtgt tcaccaagaa gaacggcacc
      901 tggaacctgg cgactcgggc ggttaccgac cccacctcgg atgatgatgc ctttggcaaa
      961 tcggttgggg tgtatcgcga ttggttcatc atcggcgcca gcggcagaga tccaaattcc
     1021 aagacgcgcg ccggcgaagc cttcctaagc cttctgggct cagttcagct ccccaatacc
     1081 gggtttgcac ctgggcagca gaccatcctg cctgagcagc catcatccct ggcttatgaa
     1141 gattacggcg agatgtggtt agagataccc tccctgcagc agaaaatgac catccagggc
     1201 gtgcccaaag gcgacaacgg ctgggatgtg cgttggctct cacggcaggc aggttatttg
     1261 gaaggcaccg cctttccaac atggtctggc aacaccggtt tggcaggtca caccgtcttg
     1321 ccggatggaa ccccagggcc atttgccaag ctgcatacat tgagttgggg tgatcaagtt
     1381 tacatccacg cctggggcaa gcgctatatt tacgaggtgc gggagaactc catggtgcag
     1441 ccgaacacac ttaaagtact caaacatgaa gagctagact gggtaacact gatcacatgc
     1501 cagggatacg acgagaaaag cggcaagtac cgctggcgcc gggtggtccg ggcagtgctg
     1561 gtggctgtcg tagaggaata ggccccaacg agctgatgag acgaggatgt gaaaaaacag
     1621 ctggcttttg ttcttggtgg gggtggagcg cgcggagcgc tacaggttgg ggcactgcgc
     1681 gccctgatgg aagccggtta ccaaccggat ttactggtcg gtacctccgc gggggcgatc
     1741 aatgccacgt ttatggcagt acgtggcatg tccctgcaaa gcatcaccga gctggtccag
     1801 gcctggcacg atgctgccac agctgaattt tttccccaga attacctgtg gcttacggtg
     1861 cgcgcccttt tccgccggcc cgatgtccac acaagtcatc gcctgaggga tttttttgta
     1921 tcccacggat taggcccgga actgcgcttt ggcgacatcc aggggattcg cctggtcctg
     1981 gtagctaccg acttgaatgc ctcacagcta gccctgtatg gcctggaccc agcggaatat
     2041 gtgctggaag gcctgctggc ttcgactgcc ctgcccccct gggtgacacc tattgagaga
     2101 gacggcagtc tcctgatgga cggcggggca atcagcaccc tgcctatcga gccggcaatg
     2161 ctcactggag ccactgaaat catcgccctc gatctatacg accccaacag ccttccgcat
     2221 gagccaaatg gctttggccc tttcctggca aaactattga atacggtgga acggcgccaa
     2281 acagagatgg agctagccct ggcttctgca cgccgcgtgc cggtgcatta cctgcatctg
     2341 cgcgccccgg actcggttcc cttgtgggat ttcagccatg ccggcgagtt aattgagatc
     2401 ggatacgagc ttgcgcgagc tcagataccc cgctggcaag cggaaagcaa gccaagattc
     2461 ttgaaagggc tgcaagggtt cattgaaggc acaattggtc catgaacaag ttgtgacccg
     2521 tgaagggagt cgtcgccccc aaggcaacgc accacccctg acagcagcca acagcctcaa
     2581 cgcacaccca gcgaataagg gcaccctgcc gcagttgaga ttttacaaag caagcgacct
     2641 caagccacat tgaacgctat tccccttt
//
LOCUS       DBNGSX010000092         3117 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1876907, whole genome shotgun sequence.
ACCESSION   DBNGSX010000092 DBNGSX010000000
VERSION     DBNGSX010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1876907"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="AC16CB_03050"
     CDS             <1..1044
                     /locus_tag="AC16CB_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HIR3341476.1"
                     /translation="QHSLDGIIAIDQQFLVALLNVIGPVNVEGSSDPISGSNVIEFMR
                     AEKTPEVQPQPQNWSRKSSIKNISVAIINKLFSSLNDVNVLAMVEMVVRMLNERHLLL
                     QIDDPQTSSILADLDWDGGIRPGSGDFIMLVDTNVGFNKVNAAVTSSINYDVNLTQLN
                     QPTSALTLTQTNHSPGTQSCAQYEKHKLEDSRYPINYCYWNYLRVYVTGETQIIDATP
                     QQISANWWLIPQKAVPARVDRLDENILGVQVYGTLMAVPVGQTNVTRFGFALPARVVS
                     CEPETKQCTYTLKIKKQPGTGAIPAKVRVYLPGAARVELLVNLTATIEDSNVMVETSL
                     RDDVIVTVVFALP"
     gene            1135..2337
                     /locus_tag="AC16CB_03055"
     CDS             1135..2337
                     /locus_tag="AC16CB_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341477.1"
                     /translation="MGKIIRFFLMLFTTKRVLYLRLMIILTLGALGAVVTATLASDVP
                     PVLVTGNPSCTDLGYAYGTKWDYPADSSGGTYSLGTGTVTWSTDGTYVDWSSTFGVGA
                     VIVKGSKAANVYYYDPESFGDTGLVSPVTSSGRAAGLSHVEFCYDIDGTGGEPPVTVS
                     GNPSCTDLGYESGTKWDYPEDSSGGTYSLGTGTVTWSTDGTYVDWSSTIGIDAVIIKG
                     GTAANVYTYDPESFGDTGLVSPVTSSGKPAGLSHVEFCYDIEGTVDVPSSYATVTEDG
                     SFALGGSVTGDVIDLAEGEEYKIEAQLIGSSSDGKLLSDDVGEFQKSIIDLKIYQNNV
                     LVDELPTKDGNVQICFAVPPDQNGTMYFLDKYFDQQAEWKAVSEPFKTGIACNPVTKT
                     GYYGMAGK"
     gene            2596..>3117
                     /locus_tag="AC16CB_03060"
     CDS             2596..>3117
                     /locus_tag="AC16CB_03060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 117 protein"
                     /protein_id="HIR3341478.1"
                     /translation="MITAVATGGVPHPTGYPTYLLMASLFQHLPFGTLAYRTNLFSAF
                     WAILAALLVYATVTYQANLSGHKNAFAGLIAGLMFGLSPLVWSQAVITEVYTLHAFFM
                     ALMLFLIVRVSIQNRHLLLDGAQGLTMGLAAGNQITSLILIPPLVFGSVRKSAKNPDW
                     GAMGRIFVGLGAGL"
BASE COUNT          809 a          697 c          804 g          807 t
ORIGIN      
        1 cagcactccc tggatggcat cattgcaata gaccaacaat ttttggttgc attgctcaat
       61 gtgatcgggc cggtaaatgt ggaaggcagt tccgatccaa tctcaggcag caatgtgatt
      121 gaatttatgc gagcagagaa aactccggaa gtccaacctc aaccccaaaa ttggtcccgc
      181 aaaagttcaa ttaagaatat tagtgttgca ataatcaata aattattctc cagtttgaat
      241 gatgttaatg tactggcaat ggttgagatg gttgtccgga tgctaaatga gcgccatttg
      301 ctgctacaga tcgatgatcc tcaaacatct tcaatcctag cagatttgga ctgggatggc
      361 ggtatcagac caggctcggg agatttcatt atgctggtgg ataccaatgt tggatttaat
      421 aaggtcaatg ctgcagttac atccagcata aactacgatg tcaatctcac acaattgaat
      481 caacccacca gcgcgctgac attaactcaa acaaaccata gccctggcac ccaatcatgc
      541 gctcaatatg aaaagcacaa actcgaagac agcagatacc ccattaacta ctgctattgg
      601 aattatttgc gagtatatgt tactggtgaa acccaaataa tagatgcaac cccacaacaa
      661 atatcggcga actggtggtt gatcccgcaa aaagcggttc cagccagggt agataggtta
      721 gatgaaaata tccttggtgt ccaagtgtac ggaacactga tggccgtgcc agtggggcag
      781 acaaatgtca ctcgctttgg gtttgccctg cctgcccggg tagtctcatg tgagcctgag
      841 acgaaacaat gtacttacac gttaaaaata aagaagcaac ccggcactgg agcgatacca
      901 gctaaagtac gggtctactt gccaggcgca gcgcgcgtgg aattgttggt aaatttgacc
      961 gcgacgattg aagactcaaa tgtcatggtt gagaccagtc tcagagatga cgtgatcgtc
     1021 accgtggttt tcgcgcttcc ctaaaattcg gactgaatcc ggatagcaaa ttccttaatt
     1081 atatagtttt ttatgatagt attcaattgg aattcttgac aaaaaggata tattatggga
     1141 aaaataattc gattcttttt gatgttgttt accacaaaac gtgttctcta cttaaggtta
     1201 atgattatcc tgacgctggg agcacttggc gcagtggtaa cggcaaccct ggcatcggat
     1261 gtgccgcccg tgttggtgac tggcaaccca agctgcaccg atctgggata tgcttatggc
     1321 acaaaatggg actacccggc agactctagt ggcgggacat attctctcgg cactggaacc
     1381 gtcacctggt cgacggacgg gacgtatgtg gattggagtt cgaccttcgg cgtcggcgcc
     1441 gtgatcgtca aaggtagcaa agcggccaat gtctattact acgacccgga gtcatttggc
     1501 gataccggtc tggtctctcc agttacctcc agtggtaggg cggccggcct gagccacgtg
     1561 gaattttgct atgatattga cggcaccggc ggtgagccgc ctgttacggt gagtggcaac
     1621 ccaagctgca ccgacctggg gtatgaatct ggcacaaaat gggactaccc ggaagactct
     1681 agtggcggga catattctct cggcactgga accgtcacct ggtcgacgga cgggacttat
     1741 gtggattgga gttcgaccat cggcatcgac gccgtgatca tcaaaggtgg caccgcggcc
     1801 aatgtctata catacgaccc ggagtcattt ggcgatactg gtctggtctc tccggtaacc
     1861 tccagtggta agccggccgg cctgagccac gtggaattct gctatgacat tgagggcacc
     1921 gtcgatgtgc cgagttctta cgccacggtt actgaagacg gctcgtttgc gttgggtggt
     1981 agtgtgaccg gtgatgttat agacctggct gaaggggaag agtacaagat agaagcccag
     2041 ctaatcggct catcgtcgga cggcaaactg ctatctgacg atgtaggaga gtttcaaaaa
     2101 agtatcattg atctgaagat atatcagaac aatgttttag tcgacgaact tccgacgaaa
     2161 gatggaaatg ttcagatttg tttcgccgtt ccccctgatc agaatgggac gatgtatttt
     2221 ctagataaat attttgatca acaagccgag tggaaagcgg tcagtgaacc ttttaagacc
     2281 ggcattgcct gcaatccagt gacgaaaaca ggctattatg gtatggcagg caaatgatca
     2341 agaaagaaat aaatagcgat aaatatgaac agaggtagaa ggatgattca atgattttca
     2401 caactctaat cggatttcga aaattgggaa aggccggtgc attaagtctt tgatagataa
     2461 tggaattgaa atattaagat gcccattcga aaagccattc caccggtttt cttagttctg
     2521 agcctgtcct taatctatct ggccacgatg gcgcccgggt taacctgggc aaatgatggc
     2581 gcggatggag gggatttgat cactgcagtt gcaacgggcg gagtaccgca tcctaccggt
     2641 tatccgacct atctgctcat ggcgagtttg tttcagcacc tgccgtttgg aaccctggca
     2701 tatcggacaa atttattttc agcgttttgg gcgattctgg ccgcgctcct cgtttacgca
     2761 acagtaacct atcaagccaa tttatcgggg cacaagaacg catttgcagg gttgattgcc
     2821 gggttgatgt tcggattatc acctctcgtt tggtctcaag ctgtaatcac ggaggtgtat
     2881 acactgcatg cgttttttat ggcgctgatg ttgttcctga tcgtccgggt ttccatccaa
     2941 aatagacatt tgctgctcga cggggctcaa ggtcttacca tgggcctggc agcgggtaac
     3001 cagatcactt ccctgatttt gatccctccg ctggtttttg gtagtgttcg taaatctgcc
     3061 aagaaccctg attggggcgc aatgggcagg atttttgttg ggttgggtgc cggtctg
//
LOCUS       DBNGSX010000093         5013 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_7149, whole genome shotgun sequence.
ACCESSION   DBNGSX010000093 DBNGSX010000000
VERSION     DBNGSX010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_7149"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="AC16CB_03065"
     CDS             <1..200
                     /locus_tag="AC16CB_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341479.1"
                     /translation="DVVIVAGVNRLSEAAFAHILSEIPDISILCIDLGTNDVQVVTNQ
                     RVSVHTTSDLLAVIGALPKQV"
     gene            215..3655
                     /locus_tag="AC16CB_03070"
     CDS             215..3655
                     /locus_tag="AC16CB_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR3341480.1"
                     /translation="MTKLTRREFLKLSGSAALSLAFTGLDLQLLEPIVVDNPLASYPD
                     RNWESIYRDQYRYDSSFTYVCSPNDTHACRLRAFVRNGIILRSETNYDVERYSDLYGN
                     RATPHWHPRGCKKGQTFHRRIYGPHRLKGPLMRKGWKEWADAGFPDLDAATKSKYKFD
                     SRGLDELLPVSWETAYDYIARGMVAIAKRYSGEAGAALLREQGYPEEMIAAMGGAGTR
                     TFKCRGGMGLLGVIGKYGMYRFANTLALVDEHVRGVGEDEAKGGRTWSNYTWHGDQAP
                     GHPFVTGLQASDEDFNDLRNSRLHIQCGKNLVENKMPESHFFIEAMERGAKIVTITPE
                     YSPPATKSDYWIPIRPATDTALFLGISRWLMDNKKYDEAFVKRFTDLPLLVRADTLER
                     LRASDLFAGYQLGLNPDGPSFKLQGLKQDQYEKLGDYVVFDNKTSAPVAITRDDVGEA
                     IDNKGIDPALEWSGELRLVDGTQVEVLTIWSMYVEHLKDYDLDAVAEITHAPKAMIER
                     LANDIATLSPVAIHIGEGINHWFHATLANRVQFLPLMLTGNIGRPGAGCYTWAGNYKA
                     ALFQGSEQTGPGFKGWVAEDPFHPNLDPKEHGKKIVAHSFTKDEEPAYWNHSERPLIV
                     DTPKYGRKVFTGNTHMPTPTKVQFFTNVNLLNNAKHHYEMIKNVNPNIELIISMDIEM
                     TSTVEYADFGLAANSWAEFQVHEITASCSNPFLQIWKGGIPPLYDTRDDVRIVAEVAA
                     TLGDQFGDPRFRDYWKFALEGKPEVYIQRLLDSSLTTSGYRFEDIVAGRYGEPGAALM
                     LFRTYPRIPFWEQLNDSVPFYTDTGRMNAYCDIPEAIEYGENIISHRESPEATPYLPN
                     VIVTSSRFVRPDDYGIPLDAEGWDERTVRNVSMAWEKVKDTKNPLWEAGYQFYCFTPK
                     TRHRVHSSWSTVDWTIILDSNFGDPYRMDKRLPGPGDHQLHMNPQAAKDMGLEDGDYV
                     YVDANPADRPYVGWKPDDPFYKVARLMLRLKYNPAYPYHIVMLKHGPFMATEKSVLAH
                     ETRPDKLAKSAGTGYQANLRYGSQQSITRDWSMPMHQTDTLFHKGKVAMQFIFGGEAD
                     NHALNTVPKETLVKITKAEPGGLDGKGPWEPTKTGFTPGHEGDFMQKYLAGGAVEIKK
                     "
     gene            3680..3943
                     /locus_tag="AC16CB_03075"
     CDS             3680..3943
                     /locus_tag="AC16CB_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341481.1"
                     /translation="MPYEEFDVEDPMDLVGMVLPGEPGQLEAMAECFVEEYVRMGWNE
                     TRLMTLFVNPMFMATHRIYRQKGEKYVKELIQRVGSKYQLKTG"
     gene            3984..>5013
                     /locus_tag="AC16CB_03080"
     CDS             3984..>5013
                     /locus_tag="AC16CB_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3341482.1"
                     /translation="MAEVYNWQLGRNMSYVFDEAHPQKQFVAIFNINRCIGCQTCTGA
                     CKATWTFSKGQEYMWWNNVETKPYGGYPQHWDIKILQILDEAHKTAGVKAEWDTSQTS
                     ENAPYGVYNGMTITEAGGDNQQALGYIPPDTEWRAPNFYEDTSTAYKGDALGISKEGV
                     SLPEHQTWFFYLQRICNHCTYPACAAACPRKAVYKRPEDGIVLIDQARCRGYRKCVEQ
                     CPYKKSMYRGTTRTSEKCVACYPRVEGTDPLTDGVPMETRCMAVCPGKIRLNGLVDIG
                     PDGSWAEDPQNPLYYMIQVEKVALPLYPQFGTEPNIYYIPPRWAPRSYLRQMFGPGVD
                     EAIARYAAP"
BASE COUNT         1167 a         1394 c         1401 g         1051 t
ORIGIN      
        1 ccgatgtggt catcgtcgcg ggggtgaacc ggctatccga ggcagccttt gctcatatcc
       61 tctctgaaat tcctgatata tcgatcctgt gtatcgatct aggcaccaac gatgtgcagg
      121 ttgtcactaa tcaacgtgtg agcgtgcaca ccaccagtga tttgctggcg gtgatcggtg
      181 cgctacccaa gcaagtataa atggagatga cctgatgaca aaacttaccc gtcgcgagtt
      241 tctcaaattg tctgggtctg cagccctcag cctggcattc accggactgg acctgcaatt
      301 actcgaaccc attgttgtgg ataacccact agcgtcttat ccggatcgca attgggaaag
      361 catttaccgg gaccagtatc gttacgactc ttcctttacc tacgtctgct ctcccaacga
      421 tacgcacgcc tgccgcctgc gggcttttgt ccgcaatggg attatcctgc gttctgagac
      481 gaactacgat gtggagcgtt acagtgacct gtacggcaac cgggcgacgc cgcactggca
      541 cccgcgtgga tgtaagaaag gccagacttt tcatcgccgc atctacggac cgcaccgcct
      601 taaaggccct ctgatgcgca aagggtggaa ggagtgggct gacgctggct tccctgattt
      661 ggacgctgcc acaaagtcca aatacaaatt tgattcacgc gggctggatg agctgctgcc
      721 ggtctcgtgg gagacagctt acgattacat cgcccgtggc atggtcgcca tcgccaagcg
      781 ctactctggt gaggcaggcg ctgcgctttt gcgggagcag ggctaccctg aagagatgat
      841 tgcggcaatg ggcggcgcgg gaacgcgtac ttttaaatgc cgcggcggca tgggattgtt
      901 aggcgtaatc ggcaaatatg gcatgtaccg ctttgccaac acgctggctc tggtggatga
      961 gcacgtgcgc ggcgttggcg aagatgaagc caagggtggg cgtacttggt ctaattacac
     1021 ctggcatggc gaccaggctc ccgggcatcc cttcgtcacg ggcttgcagg cttcagacga
     1081 ggatttcaat gacctgcgta actcgcgcct gcacatccag tgtggcaaga acctggtcga
     1141 gaataagatg cctgaatctc acttctttat agaggcgatg gagcgcggtg ccaagatcgt
     1201 cacgatcacg ccggagtatt cgcccccagc aactaagtcg gattactgga tccccatccg
     1261 acctgcgact gacactgcgc tgttcttggg aatctcccgc tggctgatgg ataacaagaa
     1321 gtacgacgaa gcatttgtca agcgatttac cgatttgcct ctcctggtac gggctgatac
     1381 cctcgagcgc ttacgcgcca gcgatctctt cgccggttac caacttgggc tgaaccccga
     1441 tgggccgagc tttaagctgc aagggctcaa gcaggaccag tacgaaaaat tgggtgatta
     1501 tgtggtcttc gataataaaa cctcagcccc cgtcgccatc accagggatg atgtgggaga
     1561 ggcgattgat aataaaggga tagatcctgc cctggagtgg tctggggagc tcaggctggt
     1621 cgatgggacc caggtagagg tgctgacgat ttggtctatg tacgtggagc atctcaagga
     1681 ttacgatctg gatgcggtgg cggaaatcac ccatgcgccc aaagcgatga tcgagcgcct
     1741 tgcaaacgac atcgccacac tcagcccggt cgccattcac atcggtgagg gtattaacca
     1801 ctggttccat gccaccctgg ctaaccgggt gcagtttctg ccgctcatgc tcacgggcaa
     1861 tatcggaaga cccggtgcag gctgttacac ctgggctggc aattacaagg cggctctctt
     1921 ccagggctca gagcagaccg gacctggctt caagggctgg gtagctgaag accccttcca
     1981 tccaaatctg gatccgaaag agcacggcaa gaaaatcgtg gcgcattcct tcaccaagga
     2041 tgaggagccg gcttattgga accattcaga gcgcccctta attgtcgata cccccaagta
     2101 cggtcgcaag gttttcaccg gcaataccca catgcccacg ccaaccaagg tgcagttctt
     2161 caccaatgtc aacctgttaa acaatgccaa gcaccattat gagatgatca aaaacgtcaa
     2221 cccgaacatt gagttgatca tctcgatgga cattgagatg acctccacag ttgagtacgc
     2281 cgactttggc ttggcggcca actcctgggc tgagttccag gtgcatgaga tcaccgcttc
     2341 atgctccaat cccttcctgc aaatctggaa gggcggaatc ccaccgctct acgatacacg
     2401 cgatgatgtg cgcatcgtgg cggaggtcgc ggctaccctg ggtgaccagt ttggcgaccc
     2461 gcgcttccga gattactgga aattcgccct cgaggggaaa cccgaggtgt atattcagag
     2521 gctgcttgac tcatcgctga ccacctctgg ctaccgcttt gaggatatcg tcgctggaag
     2581 atacggcgag ccgggcgcag cgttaatgct cttccgcacc tacccgcgca tccccttctg
     2641 ggagcagttg aacgattccg tgccgttcta caccgacacg ggccgcatga acgcttattg
     2701 cgatataccg gaagcgattg agtatggcga gaatatcatc agccatcgcg aaagcccgga
     2761 ggccactccc tacctgccga acgtcatcgt cacctccagc cggtttgtgc gtcccgatga
     2821 ttatggcatc ccgctcgatg cagaaggctg ggatgagcgc acggtgcgca atgtatccat
     2881 ggcttgggag aaagtaaagg acaccaaaaa tcccctgtgg gaagccggct accagttcta
     2941 ctgcttcaca cccaagacgc gccatcgagt gcactcttct tggagcacgg tggactggac
     3001 catcatcctt gattcgaact ttggcgaccc ctaccgcatg gacaaacgcc tgcctggtcc
     3061 aggcgatcac cagcttcaca tgaatcccca ggcggccaaa gatatgggcc tggaagacgg
     3121 cgactacgtg tatgtcgatg ccaatcccgc cgaccgccca tatgtgggct ggaaaccaga
     3181 cgacccattt tacaaagtcg cccggctgat gcttcgcctg aagtacaacc cagcctaccc
     3241 ctatcacatc gtcatgctga agcacggacc tttcatggca accgagaaat cggtgctggc
     3301 tcacgagaca cgcccggaca agctggcaaa gtcggcgggg acgggatatc aagccaacct
     3361 gcgttatggc tcgcagcagt cgatcacgcg tgactggtct atgcccatgc accagaccga
     3421 tacgctcttc cacaagggga aggtagctat gcagttcatt ttcggtggtg aagccgacaa
     3481 tcacgccttg aacaccgtcc ccaaagagac cctggtaaag atcaccaaag ccgagcccgg
     3541 aggtttggac ggcaaaggcc cctgggagcc gaccaagacc ggctttaccc ctgggcatga
     3601 aggcgacttc atgcagaaat atctggctgg tggggcggta gaaataaaga aatgattggt
     3661 gtgcacactg gaggaaagaa tgccatacga agaatttgat gtagaagatc caatggacct
     3721 ggtcgggatg gtgctgcccg gcgagccagg ccagcttgaa gcgatggcgg aatgcttcgt
     3781 ggaagagtat gtgcgcatgg gttggaatga aacacgcctg atgaccctct ttgtcaaccc
     3841 gatgttcatg gccacgcacc gcatttatcg ccaaaagggt gagaagtacg tgaaagagct
     3901 gatccagcga gtgggctcaa aatatcagct caagacaggc tagtcatgct aggaaaattg
     3961 ctcgcaccaa ggagttaatc gatatggctg aagtctacaa ctggcaacta ggccgaaata
     4021 tgagttacgt tttcgacgag gcgcaccccc agaaacagtt tgtcgccatc tttaacatta
     4081 accgctgtat tggctgccag acctgtaccg gggcttgcaa ggctacctgg accttttcga
     4141 aaggtcagga atacatgtgg tggaacaatg tggagaccaa accctatggt ggctaccccc
     4201 agcactggga tatcaagata ttgcagatac tggacgaggc tcacaagacc gctggcgtaa
     4261 aggctgagtg ggatacatcc cagaccagcg agaacgcccc ttacggtgtc tacaacggga
     4321 tgaccattac cgaagcgggt ggcgataacc agcaagcgct ggggtatatc cctccagaca
     4381 ccgagtggcg cgccccaaat ttctacgagg acacgtctac tgcatataag ggcgacgcgc
     4441 tgggtatcag caaggagggc gtatcgctgc ccgaacacca gacctggttc ttctacttgc
     4501 agcgcatctg caaccattgt acctatccag cctgtgcagc ggcttgcccg cgcaaggcgg
     4561 tttacaagcg cccagaggat gggatcgtgc tgatcgacca ggcgcgctgc cgcggttatc
     4621 gcaagtgcgt tgaacaatgc ccctataaaa aatcgatgta tcgcggcact acccgcacca
     4681 gtgagaagtg cgtggcctgt tacccgcgtg tggagggcac tgacccgctt acggatggcg
     4741 tgcctatgga gacgcgatgc atggcagtct gccctggcaa gattcgcttg aatggtctgg
     4801 tagatattgg cccggatggc tcgtgggcag aagacccaca gaacccactg tactacatga
     4861 tccaggttga aaaagtggcg ctgccactct acccgcaatt tggcaccgag cctaatattt
     4921 attacatccc gccgcgctgg gcgccgcgct cctacctgcg ccagatgttt ggtccagggg
     4981 tggatgaggc gatcgctcgc tatgcggcgc cat
//
LOCUS       DBNGSX010000094         2318 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1115243, whole genome shotgun sequence.
ACCESSION   DBNGSX010000094 DBNGSX010000000
VERSION     DBNGSX010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1115243"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..182
                     /locus_tag="AC16CB_03085"
     CDS             15..182
                     /locus_tag="AC16CB_03085"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3341483.1"
                     /translation="MYCGSVDKHVYCLEYSTGRLKWKYPTNGPITGTPIAVDGVVYIG
                     SNDHHLYALPA"
     gene            210..1106
                     /locus_tag="AC16CB_03090"
     CDS             210..1106
                     /locus_tag="AC16CB_03090"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF012693.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR3341484.1"
                     /translation="MKRFIDKLRGSQQALSQEIPTRPIPKENLETISNSPHPRPEPPQ
                     LIVGCAQSTGVQRNNNEDALFTLTTTLTGDFRNTPFGLYIVADGMGGHQHGEIASGIA
                     IRSMASYVIRKLYSPLLSLKPNLPEESLLEIMQAGVQDAHSAIIKQVPGGGTTLTAAL
                     IVGNRMTIAHVGDSRAYLIGNNGKVEALTRDHSLVNRWVELGQITLEEAAVHPHRNVL
                     YRALGQGEPFDPDINSIQIPDTGYLLICSDGLWSVVPEEEIQAIVQKSKNPEQACQML
                     VEAANAAGGPDNITVILIRLHV"
     gene            1115..>2318
                     /locus_tag="AC16CB_03095"
     CDS             1115..>2318
                     /locus_tag="AC16CB_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HIR3341485.1"
                     /translation="MEQLPDYYAQLGVSREASAEEIRRAYHRAARELHPDLNEDVNAN
                     DSFLQLQAAYEVLSDPDKRTEYDQSLPPLEEKTPAVGLNVLFSRPSLIKISEPQMIFV
                     MMELTAPPAAKAEPSPPLNVCLVLDRSTSMRGERMDTVKRTAIKLLRQLRQEDIISIV
                     TFSDRAEVLVSGGKLSNRIEIENKIKMIQPSGGTEIYHGLDAGFFEVRSRSRNNSTNH
                     IILLTDGRTYGDEAECLRIADQAAYSGIGISGLGIGSEWNDVFIDSIAARTGGSSLYI
                     SNPSEIEKALNEVISGLRQIYAERVSFEFVAQPGVEMHYAFRLSPQANPLKTSSPIRL
                     GRIPMEADLTFLLEFIVDPIDKTSDALTLIEGQISMDIPTRANTEHTIPLHLSGVISQ
                     ESSTEPPPT"
BASE COUNT          619 a          611 c          557 g          531 t
ORIGIN      
        1 tctataacga ttcgttgtat tgtggatcag ttgacaagca tgtttattgc cttgaataca
       61 gcacagggcg gctaaagtgg aagtacccca caaacggacc aattaccgga acgccaattg
      121 ctgtcgacgg tgtagtatac atcggttcaa atgatcatca cctctatgca cttcctgcat
      181 agtagttaaa aacagagaag gtaaactacg tgaagcgctt catcgacaaa ttgcgcggct
      241 cccagcaagc cctgagccag gagataccga cgcggccaat tcccaaagaa aacctcgaga
      301 cgatatcgaa ctcacctcat ccgcgccccg agcccccaca gctaattgta ggttgtgcac
      361 aatcaacagg agtacagcga aacaacaacg aagatgcctt attcaccctt accaccaccc
      421 tgactggcga ctttagaaat actcccttcg gcttgtatat cgtggctgat gggatgggcg
      481 gacaccagca tggggaaatc gccagtggca ttgccatccg ttcgatggct agttacgtta
      541 ttcgcaagtt atattcccct ctactgagcc taaaaccaaa tctccccgaa gaatcgctcc
      601 ttgaaattat gcaggcaggc gttcaagacg ctcactctgc gatcattaag caggtgccgg
      661 gcggtggcac caccctgacc gcggcattga tcgtcggcaa ccgtatgacc atcgcacatg
      721 taggagatag ccgcgcatac ctgattggca acaacggaaa agttgaggcg ctaacacgcg
      781 accattcttt ggttaatcgc tgggtggaac tgggacagat cacactcgaa gaagcagctg
      841 tccaccctca ccggaatgtg ctctatcgag ccttgggtca aggcgaacca tttgatcccg
      901 atattaattc aatacaaata cctgacacgg gttacctgct tatttgctct gatggacttt
      961 ggagtgtggt gccagaagag gaaatccaag ccatcgtaca gaagtcgaag aatcctgaac
     1021 aggcttgcca aatgctggtg gaggctgcca acgcggctgg tgggccagat aacattactg
     1081 tcatccttat ccgtttgcat gtataacagc ctttatggag caattacctg attactatgc
     1141 gcaactgggg gtgtctcgag aagcctcagc ggaggaaatt cgccgtgcct accacagagc
     1201 cgctcgcgag ctccatcctg acctaaacga ggatgtgaac gcgaacgact ctttcctaca
     1261 acttcaagcc gcctatgagg tgctatccga tccagacaag cgtaccgaat acgaccaatc
     1321 tttaccaccg ctggaagaga aaacgcccgc agttggcctc aatgtcttgt tcagccgccc
     1381 ttcgttgatc aaaatcagcg agccgcaaat gatatttgtt atgatggagc tgactgcccc
     1441 gccagcagcc aaggcagaac ccagcccccc tctcaatgta tgtctggtgt tggatcgctc
     1501 gacttccatg agaggcgagc gcatggatac ggtcaaaaga acagccatca aattgctccg
     1561 ccagctccgc caagaagaca tcatatccat tgtgactttc agtgatcggg ctgaggtttt
     1621 agtctctggc ggaaaactct ccaatagaat cgagattgaa aacaagatca agatgataca
     1681 gcccagtggt ggcaccgaaa tctaccacgg gttggatgcc ggtttttttg aggtgcgcag
     1741 ccggagcaga aataactcca ccaaccacat tatcctgctc acggatggtc gcacctatgg
     1801 agacgaagcc gagtgcttgc gtattgctga ccaagctgcg tactccggaa taggaattag
     1861 tgggcttggg atcggttctg agtggaatga tgtattcatc gatagcatcg ctgctcgcac
     1921 cggtggcagt agcctgtata tctccaatcc gagtgaaatc gagaaagcct tgaacgaggt
     1981 aatttcagga ttacggcaga tatatgccga acgagtgtct tttgaatttg ttgctcaacc
     2041 tggagttgaa atgcattacg cttttcggct ctccccacaa gctaaccccc tgaagaccag
     2101 ctctcctatc cgtttgggca ggatcccaat ggaagcagat ttgacatttt tgcttgaatt
     2161 tattgtagac cccatcgata aaaccagcga tgcgctgaca ctcattgagg gacaaatttc
     2221 gatggacatt cctactcgag ccaatacgga acacacaatt cctttgcacc tatccggggt
     2281 gattagccag gaaagcagta ccgaacctcc tccaacac
//
LOCUS       DBNGSX010000095        14305 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_976817, whole genome shotgun sequence.
ACCESSION   DBNGSX010000095 DBNGSX010000000
VERSION     DBNGSX010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14305)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14305)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14305
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_976817"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..111)
                     /locus_tag="AC16CB_03100"
     CDS             complement(<1..111)
                     /locus_tag="AC16CB_03100"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR3341486.1"
                     /translation="MEENRLSSKLYRALITGGAGFIGSHLAEALLSRGHHV"
     gene            complement(111..1175)
                     /gene="gmd"
                     /locus_tag="AC16CB_03105"
     CDS             complement(111..1175)
                     /gene="gmd"
                     /locus_tag="AC16CB_03105"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR3341487.1"
                     /translation="MKKALITGITGQDGSYLAELLLSKGYEVHGLIRRASTFNTQRID
                     HIYRDPHAQNGVNFHLHYGDIAASGNLIELVYNVQPDEIYHLAAQSHVRVSFDLPEYT
                     GDVTGLGAMRILEAIRKSGIKARFYQASSSEMFGSAPPPQSENTAFEPQSPYAAAKLY
                     AFWVTRNYRQAYDIFASNGILFNHESPRRGETFVTRKITRALAAIKAGKQQYLYLGNL
                     DAKRDWGYAPDYVAAMWKMLQHDQAGDFVIGTGEAHSVREFLDEAFGYLNMDWKDCVK
                     IDPRYYRPTEVDYLLADPGKAQRELGWEPRVFFKDLVHIMVDADLELIGLESPGEGKR
                     ILEQRHGKWHRWESQVVSME"
     gene            complement(1172..2542)
                     /locus_tag="AC16CB_03110"
     CDS             complement(1172..2542)
                     /locus_tag="AC16CB_03110"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR3341488.1"
                     /translation="MTLDELKIKIQAKEARLAVIGLGYVGFPVACEFARLGFDVLGVD
                     LRRERVDMINSGQSPIVGNEPGLSELAAEVIGKQRFKAVFDYEQMADRDVIIICVETP
                     VDEHNIPRYTALRSALNELGPVLKDGALVIVESTIAPRTMQDVVLPNLEQATLKRLNE
                     GFYLGNCPERVMPGKLLANIRGVHRVVGGMTPETAQVIALLYRHIVHAEIDQTDCITA
                     EVVKTVENTYRDVQIAFANEVALICEQVGGDVWRVRELVNKSPGRMMLMPGAGVGGHC
                     IPKDPWLLAYSVHEDDIQLRIIPAARSVNNYMPYHMVELIKKGLESIGSQIPGAKILV
                     MGYSYLEDSDDSRNSPSKVLIGYLEDQDADCIIHDPFIEDYQGSLLDKAQGCDAAVLM
                     VGHTEYRKLDLKALSGVMKTPVLIDGRSVFDPATAARAGFTYWGVGVHPEAHEKIQMN
                     EVKPIQ"
     gene            complement(2529..3545)
                     /locus_tag="AC16CB_03115"
     CDS             complement(2529..3545)
                     /locus_tag="AC16CB_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIR3341489.1"
                     /translation="MERLRVAVIGTGSIGRNHARVFSELPEVELAAVCDTDSSLVNDI
                     ADRYRTRAFTNYQEMLEKIKPDAVTIAVPTASHIEVAMAALEAEVDLLVEKPIAATLD
                     EGRKLIEKARSKSRLLMIGHIVRFNPAIQELKRRLQSGEVGRIFQIISRRVGPFPARI
                     RDVGVVIDLAPHDLDMMRFLTDMEPIRVFCETEQRIHTDHEDLLLGLLRFPEGITAAI
                     EINWLTPTKVREIIVLGERGMFRVDDLTQDLYFYENAEVGEKLWPALQNLKGVSEGSM
                     TRYAVKRYEPLKAELSAFVRAIKDNTPSPVSGEDGLAALRLALALVESGLKHQVIEVL
                     HDAG"
     gene            complement(3559..4077)
                     /gene="nusG"
                     /locus_tag="AC16CB_03120"
     CDS             complement(3559..4077)
                     /gene="nusG"
                     /locus_tag="AC16CB_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014417.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0031564 - transcription antitermination
                     [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIR3341490.1"
                     /translation="MTDYWYALRSKPRKEEVVWRQVRTQGIEVFYPRLRVQPVNPRSK
                     KWRPYFPGYMFIKVDIDQIGLSSFQWMPHAIGLVCFGDEPAIVPENLIFAIKKRVDEI
                     AAIGGEVFDGLNPGDIVMIDGGPFTGYEAIFDARIEGSERVRVLLQLLNDNRQVPLEL
                     DATQIKQKKRSK"
     gene            complement(4364..5134)
                     /locus_tag="AC16CB_03125"
     CDS             complement(4364..5134)
                     /locus_tag="AC16CB_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341491.1"
                     /translation="MFSFVLKNLDGLWWLLIFLGPFLILQRRLHMEIQSVLILLTHRN
                     DISLAIFSLLFFPGVLLHELSHFLLARLMGVRTGGFSLIPRPIEDGRLQLGYVETAKT
                     DFVRDALIGAAPLLAGGLFVAWVGIVHLGLLGIWDSLNSTDEQALSLAVQDLLVRSDF
                     WLWFYLIVAVSSTMMPSRSDRRAWLPVALIVAFLLGLGIFAGAGPWLLDNITAPLNNA
                     LRTVAIVFAISMGVHLVLVMPFIFLRRVLERLTGYEIA"
     gene            complement(5140..5916)
                     /gene="tpiA"
                     /locus_tag="AC16CB_03130"
     CDS             complement(5140..5916)
                     /gene="tpiA"
                     /locus_tag="AC16CB_03130"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR3341492.1"
                     /translation="MRTPLIAGNWKMYNTIAEARHLVSEMVPGLQAIKSVEKVLCPPF
                     TSLLAVRSLLEGTEIGLGGQDVYWEASGAFTGEVSPTMLAELCQYVIIGHSERRAYFG
                     ESDVSVNRKVHASLAHRLKPIVCVGETLDENEAGKTTEVVTRQVKVGLQGLDSSTIGL
                     GDGNISIIIAYEPIWAIGTGRAATAEDANGVVANVIRPILKEMFNETIAQAIRVLYGG
                     SVKASNAGEFFQQPEIDGALVGGASLKATEFVSIVQAASR"
     gene            complement(6144..7319)
                     /locus_tag="AC16CB_03135"
     CDS             complement(6144..7319)
                     /locus_tag="AC16CB_03135"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR3341493.1"
                     /translation="MFNKKSITDISVSGKRVLVRVDYNVPIKDGKVDDDTRIRAAMPT
                     LDYLLDHGASVILCSHLGRPKGEPNPKYSLQPVAEHLGRLMAKPVMFAEDCIGPSAEK
                     AARALNAGDVLLLENTRFHPEEENNDTNMARQLAGLADIFVNDAFGSAHRAHASTEGV
                     AHFLPAVAGFLMEKEIRYLGQAIDNPKRPFYAILGGAKVSDKIGVIRNLLQKTDQVLI
                     GGGMANTFFKAEGYPVGDSLVEDEALDIARELIQKSASHLRLPVDVVIGDRFDAEAER
                     KVMPMGPIPDGWRILDIGPESVANFQKVLSTAGTIVWNGPMGVFEFPRFAEGTFGVAK
                     AVAASKAISIIGGGESVSAIQKSGLADKITHISTGGGASLEMLEGLVLPGLAALNDK"
     gene            complement(7388..8443)
                     /gene="gap"
                     /locus_tag="AC16CB_03140"
     CDS             complement(7388..8443)
                     /gene="gap"
                     /locus_tag="AC16CB_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR3341494.1"
                     /translation="MKVKVGINGFGRIGRQVLKAIGDYHPDSLEVVAFNDIGDLKTMA
                     HLLKYDSNYGRFDGEIELGKDELIINGKSVKVFKETDPAKIPWGDLGVEIVIESTGIF
                     TIKKDGVNKKGKQVQGAENHITKGGAKKVIISAPAEGEDLTVVLGVNDDKYDPAVHHV
                     ISNASCTTNCLAPAAKVVNDKFKIKRAFMTTIHAYTNDQRILDLPHSDLRRARAAAMN
                     IIPTTTGAARALKLVIPELAGKFDGYALRVPTSTVSVVDFTADVEIATTTEELRQAFR
                     DAAAGPMKGILAAVEEPLVSVDFKGDPHSSSVDLAFTTVLGKEKSDFVKVVTWYDNEW
                     GYSVRTADLAAMMARSL"
     gene            complement(8555..9889)
                     /locus_tag="AC16CB_03145"
     CDS             complement(8555..9889)
                     /locus_tag="AC16CB_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016869756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HIR3341495.1"
                     /translation="MEKDSAPLYRRWLNAAARSLRWLVPGLGVKRWLLVILAGTTLIG
                     VGLAIIILDIYRTTPDTWWLPLLSTASLRFIARPLRAIIFGGLGLGLIITGIYGLNRT
                     ILRPFLRPGRHVVDAITQHRRKERGPRVVAIGGGNGLSTLLRGIKSYTHNITAIVSVA
                     DDGGSSGRIRREIGILPPGDIRNCLAALSSDEALLTQLFQYRFPEGNEDLVGHSFGNL
                     FISALADLTGSFEDAVAESGRVLAVNGRVVPSTLHDVRLEADLKLPHLVSEVRIKGES
                     HIPEASGSIRRVWLEPNNPPAFPQAVRAILAADLIIVGPGSLYTSILPNLLVPDLLEA
                     IRASRALRIYVCNVATQPGETQNYTCGDHIRAIEEHVGDGLFEVIVANNNYESDLPGP
                     SSWVRVEDDLDVDHAVYRADLIANDFPWRHDSDKLATVLMDILAERTGPLVE"
     gene            9962..10894
                     /locus_tag="AC16CB_03150"
     CDS             9962..10894
                     /locus_tag="AC16CB_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR3341496.1"
                     /translation="MSVHIAVDIGGTHMRAAYYPAHSHEPLEHKKITTKHPSESAWER
                     LVKLITSIWPAGEEVASIGVAAPGPLDPFSGVLFSAPNIHEWINLPLRKLLEDQFEVP
                     IAVGNDANLAALGEWKFGAGIGHKHLVYLTISTGIGGGVIIDNRLLVGTRGLTAEFGH
                     VSVQPDGPLCGCGQRGHLEALAAGPAIARWVETEIAQGVPSSLAKLAQFTTKQVAEAA
                     NQGDDLAIRALNRAGNFIGRALADILHIFNPSIIIIGGGVSRSGDLLMHPIRKEMEAR
                     VMNPHYLDNLTLTTAALGDETGLLGALALARDIK"
     gene            10894..12573
                     /gene="recJ"
                     /locus_tag="AC16CB_03155"
     CDS             10894..12573
                     /gene="recJ"
                     /locus_tag="AC16CB_03155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR3341497.1"
                     /translation="MEKRWIIPPRLPLEVDHALHGYPPILRQILYNRGYATHEDARSF
                     LEARTPPGTDPLNLSGVAPAVERIQSAIRHAEPIAIYGDYDADGVTATALLVEVLNRL
                     GADVRGYIPNRFDEGYGLNNEALSSLRDQGIRLIITVDCGIRSVSEAEHARQLGIDLI
                     ISDHHHPGPEIPHAFAVINPKLPGDTFPEKDLAGVGLAYKLACALIGDDQAESSTDLL
                     DLVALGTVADMAPLVGENRALVRAGLEVLRRPRRQGIRSLMGASGLMPDKINASHIGF
                     VLGPRLNAAGRLDTALAALNLLLTSDLGEAGILSQQLDMYNRERQEITRLIQESAEQM
                     VLADDPQALLLVAAHSDFNAGVVGLAASRLCEKYYRPSIIATRGEEFTRASCRSIPEF
                     HITQALDQCADLLEHHGGHAAAAGFTVVNENVDELIARLRLIANQELGELDLRPVLQS
                     DMELPLNELKPELLNYLDWLQPTGYGNRAVHFVSRNLRVTRSQAVGRDGSHLKMSVSD
                     GRITYDAIAFRQGHWLEDLPTHVDLLYEFETNEYNGRVSLQLNVRDIKPSS"
     gene            12638..13987
                     /locus_tag="AC16CB_03160"
     CDS             12638..13987
                     /locus_tag="AC16CB_03160"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3341498.1"
                     /translation="MTAFRPANPAFSTSKPSGGHFVPPPLSLIEDTGLSALWLQDLAL
                     KIMYFQGYMTGFKMAEEIALPFNGIVDQILESLKREKFVEVKSSQQMGLGEGSYQYAL
                     TGAGIARAREAMERSQYAGPAPVPLKVYNESILRYSKGRPMVGSRDLRQALSELVLNE
                     RTYQRLGPAINSGTSIFLYGPPGNGKTTIARAVGQMILRESMYIPYALYVDGQVVKLF
                     DSVNHQLLRDEDSTPLGTGSLRSGSRRDPRWLRIRRPFIVVGGELNMESLDLVFDDVN
                     KFYEAPFQVKATGGILLIDDFGRQQIRPRDLLNRWIVPLENRIDYLTLHTGRKVEIPF
                     DVLVIFSTNLPPKDLVDEAFLRRLRHKIEIGDPNYNDYREIFRRVAVAKDITYSDRGL
                     AYLLQEWYIKRGRKMRASHPRDLCDQILDIARYLSVEPAMTRDLLDRAAESYFVELY"
     gene            13999..>14305
                     /locus_tag="AC16CB_03165"
     CDS             13999..>14305
                     /locus_tag="AC16CB_03165"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003212355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR3341499.1"
                     /translation="MLDQLPNPAIIAHRGASAYAPENTLAAFELAVRQKADAIELDAK
                     LTLDNHVVVFHDQTLERTTGVHGRVIGSKLADLRRLDAGSHFDVAYWGETIPTLDEVF
                     "
BASE COUNT         3606 a         3829 c         3301 g         3569 t
ORIGIN      
        1 gacatggtgt cctcgtgata gcaacgcttc tgctagatgc gatccaatga agcctgcccc
       61 gccggtgatc agggctcgat ataatttaga ggataatcta ttctcttcca tcattccatg
      121 ctaactactt gactttccca acggtgccat tttccatggc gctgctcgag aatgcgcttt
      181 ccctcccccg gagattcgag gccaattaat tcaagatcgg cgtccaccat aatatgcacc
      241 aggtctttaa agaacaccct tggctcccag cccaactccc tttgagcctt tccggggtct
      301 gccagcaagt aatccacttc agttgggcgg taatagcgcg ggtctatttt tacacaatct
      361 ttccagtcca tgttgagata accgaaagcc tcatcaagga actcacgcac cgaatgggcc
      421 tcgcccgtgc caatgacaaa atcacccgct tggtcatgct ggagcatctt ccacatagcc
      481 gccacgtaat ctggggcgta tccccaatct ctcttagcat ccagattgcc cagatagaga
      541 tactgctgct tgcccgcttt gatggcggcg agggcgcggg tgatcttgcg ggtcacaaac
      601 gtttcgccac gtcggggcga ctcgtgatta aataatatcc cgttgctggc aaagatatca
      661 tatgcctgac ggtagttgcg agtgacccaa aaagcataga gttttgccgc tgcgtacgga
      721 ctctgtggtt caaatgccgt attctcgctc tgcggtggtg gcgcgctacc aaacatctca
      781 ctggacgaag cctgataaaa gcgcgccttg atgccactct tgcgaatcgc ctccaagatg
      841 cgcatggcac ccaaccccgt cacatcgcca gtatattccg gtagatcaaa gctcacccgt
      901 acatgcgact gggcagccag atggtagatc tcatccggtt ggacattgta aaccagctca
      961 atcaagttac cagatgcggc aatatcccca taatgcaggt gaaagttaac gccattttga
     1021 gcatggggat cgcggtatat atgatcaatg cgttgtgtat tgaaggtgct tgcccggcga
     1081 attaatccgt gaacctcata tcctttggaa agcaacaatt cagccagata agacccgtct
     1141 tggcctgtga ttccagtaat caacgctttt ttcattggat tggttttacc tcattcattt
     1201 gaattttttc atgtgcttca ggatggacac cgacccccca gtatgtgaaa ccagctctag
     1261 cagccgtcgc tggatcaaaa acagatctcc catcaattaa tacgggtgtc ttcatcacac
     1321 cagacaacgc cttgaggtcc agcttgcggt attccgtgtg ccccaccatt aacactgctg
     1381 catcgcaccc ctgtgccttg tccagcaatg atccctgata gtcttcgata aagggatcat
     1441 ggatgatgca atcagcatcc tgatcttcca gatacccaat aagaacttta ctggggctat
     1501 tgcgtgaatc gtcagagtcc tctagataag agtaacccat aaccagaatc tttgcacccg
     1561 gaatttggct tccaatgctt tctagcccct tcttgatcag ctcaaccata tggtaaggca
     1621 tatagttgtt cacagatcgt gctgcaggaa tgatgcgtaa ttggatatca tcctcgtgta
     1681 cgctatatgc cagaagccat gggtctttgg gaatacagtg ccctcccacc cctgctccgg
     1741 gcatcaacat catcctgcca gggcttttat tcactagctc acgaaccctc caaacatctc
     1801 cacccacctg ttcgcagatc aatgccacct cattagcaaa tgcaatctgg acatctcgat
     1861 atgtgttctc aacggtcttt accacctcag ccgtgatgca atccgtctga tcaatctcgg
     1921 cgtgcactat atggcggtat aacaatgcaa ttacctgcgc tgtttctggc gtcatgccac
     1981 ctaccactcg atgtacccca cgaatattgg ccagcagttt gccaggcatt actctttctg
     2041 ggcagtttcc aagataaaac ccctcattta gccgcttcaa agtggcttgc tccaaattgg
     2101 ggagtacgac atcttgcatc gttcttggag ctatggttga ttcaacaatg acgagtgcgc
     2161 catctttaag cacgggtcca agttcgttta gagcggatcg caaggccgtg tagcgaggga
     2221 tattgtgttc atcgacgggg gtttcaacgc atatgatgat cacatcccga tctgccattt
     2281 gctcatagtc aaacacagcc ttaaacctct gcttgccgat gacttccgcg gcaagctcac
     2341 ttaacccagg ctcgttgcca acaatgggcg attgtccaga gttgatcata tcaactcgct
     2401 cccggcgtag gtcaaccccc aagacatcaa acccaagacg agcgaattcg caggcaactg
     2461 gaaatccgac ataacccagt ccaatgacag ctaaacgcgc ttctttggct tggattttta
     2521 ttttgagctc atccagcgtc atgcagcacc tctatcacct gatgttttaa gcctgactca
     2581 accaaagcca gcgccagccg taaagccgcg agaccatcct ctccgctgac tggagatggg
     2641 gtgttatctt ttattgcgcg tacaaaagcc gacagctcag ctttgagtgg ctcgtagcgc
     2701 ttgacagcgt aacgcgtcat gcttccttca ctgacacctt tgagattctg tagagcaggc
     2761 cagagttttt ctcccacttc cgcattttcg taaaaatata gatcttgggt taaatcatca
     2821 acccgaaaca tcccacgctc acccaataca ataatctcgc gaactttggt cggagtcaac
     2881 cagttgatct caatggccgc ggtaatccct tcagggaagc gcaataaacc cagcagcaaa
     2941 tcttcgtgat cggtgtgaat gcgctgttca gtttcacaga atacacggat gggctccata
     3001 tctgtgagaa agcgcatcat atcgagatca tgtggagcca gatcaattac cacacccacg
     3061 tcgcgtatgc gcgccggaaa tgggccaaca cgccgggaga ttatttgaaa gatacgacca
     3121 acctcaccac tttgtaatct gcgtttgagt tcttgaatgg cggggttaaa tcgcacaata
     3181 tgaccaatca tcaacaagcg gcttttagaa cgggcttttt caatgagttt gcgtccctca
     3241 tccaatgtag cagcaatcgg tttctcgacc aacaaatcta cctcggcttc caacgctgcc
     3301 atggcgacct caatatgaga ggctgttggg acagcaatgg ttactgcatc cggttttatt
     3361 ttttcgagca tctcttgata gttcgtaaaa gccctcgtcc tgtagcgatc agcgatgtcg
     3421 ttcaccaaag atgaatccgt atcgcatacc gcagcaagct ccacctctgg cagctcactg
     3481 aagacgcggg catggttacg cccaattgag cccgtcccga taacggctac tcgcaatctt
     3541 tccatgaaga ttcctctatt attttgatcg cttcttttgc ttaatttgcg tggcatcaag
     3601 ctcaagcggt acctgtctgt tgtcgtttag caactgcaac agcacccgca cacgctcgct
     3661 tccttctatg cgagcatcaa atattgcttc ataaccagta aaaggtcccc catcaatcat
     3721 cacgatgtcg ccgggattca agccatcaaa tacctctcct ccgatagcgg cgatttcgtc
     3781 aacccttttt ttaatggcaa agatcaggtt ctcaggcaca atagcaggtt catcaccaaa
     3841 acaaaccaaa cctattgcgt gtggcatcca ttgaaatgat gataacccaa tttgatcgat
     3901 atccacttta ataaacatat agccagggaa ataaggtcgc cactttttgg agcgggggtt
     3961 gactggttgc actcgcaagc gagggtaaaa cacttcaatt ccctgagtcc ggacttgacg
     4021 ccagacaact tcctctttcc tcggcttact gcgcaaagcg taccaatagt cggtcatatt
     4081 cctacccggt ataagtccaa gattgcatat tcaaggaggc caaggaactc aaaatctcta
     4141 aatgttgagg ggcaattttc tcaatgatca gctgatcttc gccacaagct gctggtgaaa
     4201 ttgaagcgcg gattttgccc gccatattct ctttattcaa tcccccactt cgcaacttct
     4261 ggaataagtg attaaggtta cacatgaata ataactcgct aagatgctca taattgtacc
     4321 aataaaagtt ataggtacta tcgcagcttc actaggggtt tagctaggct atttcatagc
     4381 cagtcaaccg ttccagcacc ctccgcaaga aaataaaagg catcaccaac acaaggtgca
     4441 cacccatact gatcgcaaac acgatggcta ccgttctcaa tgcattgttc aagggagctg
     4501 tgatattgtc caataaccaa ggaccagctc cagcgaagat acctaaaccc agaaggaaag
     4561 cgacgattaa cgctactggc agccacgcac gacggtctga ccgggaaggc atcattgtac
     4621 tgctaacggc tacaatcaga taaaaccaca accagaaatc tgatcttacc agcaaatctt
     4681 gtacagccaa acttaatgcc tgctcgtcag tgctgttcaa gctatcccag atacccagca
     4741 aacccaagtg gactattcct acccaagcta cgaaaagacc acctgctaag agaggagcag
     4801 ccccaatcaa cgcgtcacga acaaaatccg tcttggcagt ttcaacataa cccaattgta
     4861 gccgtccatc ctctatgggg cgcgggatca gcgaaaatcc tcctgtgcga actcccatca
     4921 ggcgtgcaag aagaaaatga gatagttcat gcagaagcac acccggaaaa aagagcaagg
     4981 agaagattgc cagggagata tcgtttcggt gagtgagcaa gattaatacc gattggatct
     5041 ccatgtgcag tcgtcgctgc aaaatgagaa atggaccgag gaagatcaga agccaccaca
     5101 atccatctag gttttttaga acgaatgaga acaagttact taccggctgg cagcttgtac
     5161 aatagacaca aattccgtcg ccttcaaact cgccccaccc accaaagcac catcaatctc
     5221 gggctgttgg aagaattcgc cggcattgct ggcctttact gacccaccat acaaaacacg
     5281 aatagcttga gcaatggttt cgttaaacat ctcctttaaa atgggacgaa ttacattggc
     5341 aactactcca tttgcatctt ctgccgtagc agccctccca gtgccgatcg cccagattgg
     5401 ctcgtatgcg atgattattg aaatattccc gtcgcccaat ccaatggtgc tcgaatctaa
     5461 gccctgcaag cccactttta cctgacgggt aacaacctct gtggttttcc cggcttcatt
     5521 ctcatccagt gtctctccca cgcaaacgat tggcttcagt cggtgtgcca aggatgcgtg
     5581 tacctttcgg ttcacggata catcggattc gccaaaatac gcacgcctct ccgaatgtcc
     5641 aattatcaca tactggcata attctgccag catagtcggt gatacttctc cagtaaaggc
     5701 gccagaagcc tcccaatata cgtcctgtcc acccagacca atctcagtgc cttcaagtaa
     5761 ggaacgtact gccaacaaag atgtgaatgg cgggcagaga actttctcaa ctgacttgat
     5821 cgcctgcaaa cctggcacca tttccgatac cagatgccgc gcttcagcga tggtgttata
     5881 cattttccag tttccggcaa taaggggcgt tcgcataatc tcctcaaagg tatttttggg
     5941 tagctcaatg cttacgatat actctacaag tcaatcctgc ttggagtttc ttgatatctc
     6001 gtaaatgtac ccaatggata aaggcatatt tagaaggatt tctatccttc gatcaccggg
     6061 ggatgaaccg gatttggatc gaacgagatg gatcattgac gggagacaaa gatcatggat
     6121 atatctttgt ctcccgtcat caattactta tcgtttaatg ccgcaagtcc aggcaatacc
     6181 agaccctcca gcatctccaa ggaagcccca cctccagtcg agatgtgggt gattttatct
     6241 gccaatcctg atttctggat cgctgagacc gactctcctc ctccaatgat actgatagct
     6301 ttgctagctg ccactgcctt cgctaccccg aaggtacctt cagcgaaacg aggaaattca
     6361 aacacaccca ttggaccatt ccatacaatg gtgccagctg ttgataaaac cttttgaaag
     6421 ttagccacac tctctggacc aatatcaagt atacgccagc catccggaat cggtcccatc
     6481 ggcatcactt tacgctctgc ctcggcgtca aaccggtcac caatgaccac atctaccgga
     6541 aggcgcagat gagacgcact tttttggatt aactcccggg cgatatctag cgcttcatcc
     6601 tccacaagag aatccccaac aggatatcct tctgccttga aaaaggtatt cgccataccg
     6661 ccgccgatca atacctgatc ggttttttgg agcagattgc gaatcacgcc aatcttatcg
     6721 ctgacctttg ctccacccag tatggcatag aaaggacgct ttgggttatc tatggcctgc
     6781 cctaggtagc gaatctcttt ttccatcaga aaaccagcta ccgccggcag gaaatgagct
     6841 acaccctccg ttgaagcatg ggcgcgatgc gcagagccaa atgcatcatt cacaaagata
     6901 tctgccaatc cagccaactg tctggccatg tttgtatcgt tattctcttc ctcgggatgg
     6961 aaacgcgtat tttccagcaa caatacatcg cccgcgttga gagccctggc agctttctca
     7021 gcagaaggtc caatgcaatc ttcggcgaac attactggct tcgccatcaa tctgcccaga
     7081 tgctctgcta caggctggag ggaatattta ggatttggct cacccttcgg tctcccaaga
     7141 tgggagcaca agatcactga tgcaccatga tccaatagat aatccaaagt gggcattgct
     7201 gcccggattc gagtatcgtc atcaacctta ccatctttaa tgggtacgtt gtaatccacc
     7261 cttacaagca cgcgcttgcc acttacactg atatcagtga tggacttttt attgaacata
     7321 ggcgcctcat tatcgcggaa tttcgagatt gcaatttttt gcacctcgaa ctccgcgctt
     7381 aattctccta taaggatctc gccatcatcg ctgccaaatc agccgtacgt acagagtacc
     7441 cccactcatt atcataccag gtgacgactt tcacgaaatc gcttttttct ttgccaagca
     7501 cagttgtgaa ggccagatct actgatgaac tgtgcgggtc gcctttgaaa tcaacactca
     7561 caagtggctc ctcaacagca gccaggatgc ctttcatcgg acccgcagcc gcatcgcgga
     7621 acgcctgtcg cagttcttca gtggttgtcg caatttcgac atcggcagtg aaatccacaa
     7681 ccgatactgt ggaggttggg acacgtaacg cataaccatc gaatttacct gccaattcag
     7741 ggatcaccag ctttagtgcc ctggctgcac cagtagtcgt gggaataatg ttcatggctg
     7801 cagcacgggc gcgccgtagg tcgctgtgag gcagatctag gatgcgctgg tcattggtat
     7861 aggcatgaat ggtggtcatg aaggcgcgct tgattttgaa tttgtcgtta accacctttg
     7921 cggccggggc aaggcagttt gtcgtgcagg atgcgtttga tattacgtga tggactgcgg
     7981 ggtcatactt gtcgtcattg acacctaaaa ccacggtcag atcctcgcct tccgcaggtg
     8041 cggagatgat cacttttttg gctccccctt tagtgatgtg attttcggct ccctggacct
     8101 gtttcccctt cttattgacg ccatctttct taatcgtgaa gattcctgtg ctttcgatga
     8161 ctatttctac ccccaaatcc ccccacggaa ttttcgccgg atcggtttcc ttaaagactt
     8221 taacggattt tccgttgatg atcaactcat cttttccgag ttcgatctca ccatcaaatc
     8281 ggccatagtt cgagtcgtat ttgagtaagt gtgccatcgt cttcaaatca ccgatgtcgt
     8341 tgaaagccac gacctcaagt gaatcaggat ggtaatcgcc gattgccttg agaacctgcc
     8401 gtccaatacg gccaaatccg ttaataccca ctttgacttt catttttgcc tccgatcgta
     8461 aagtttcatc ttgttgtgta taaaattggt gggtttcgac caccgaatca tcaaattaat
     8521 aaatagattt caatttatcc cagatacata cgtcttattc tactagcgga ccagtccgct
     8581 cagctaatat atccatcaat actgtggcga gcttatccga atcatgccgc catgggaaat
     8641 cattggctat cagatccgca cggtagacag catggtcaac atccagatca tcttccaccc
     8701 gcacccagct cgatggtcct ggtaaatcac tttcataatt attattcgcc acaatgacct
     8761 cgaacaagcc atccccgaca tgttcctcaa tggcacgaat atggtcgcca cacgtataat
     8821 tttgcgtttc accaggctga gtggcgacat tacacacata tatccttaaa gcccggctgg
     8881 cacgtattgc ctcaagtaga tctggcacga gcaaatttgg caaaatgctg gtatataggc
     8941 tgccaggtcc aacgataatc aggtcagcag ctaaaatcgc acgcaccgct tgagggaaag
     9001 ccggcgggtt atttggttcc agccagacgc ggcgaatgct gcccgatgct tctggaatgt
     9061 ggctttctcc ctttatgcgc acttcactca ccaggtgcgg taattttaaa tcggcctcca
     9121 agcgcacatc gtgcaacgta gagggaacca cccgaccatt cactgccaga acacgccctg
     9181 attctgctac cgcgtcttca aagcttccgg tgagatcagc cagagcactg ataaacaggt
     9241 taccgaagga atgacctaca aggtcctcat taccctcagg aaagcggtat tggaagagtt
     9301 gggtgagcaa cgcttcgtcg cttgagagtg cagctaggca gttacggata tcgccgggag
     9361 ggagaatacc aatctcccgc cggatacgtc ccgatgaacc accatcgtca gctacagaga
     9421 cgatcgcggt gatattatgc gtgtagctct tgatgccccg aagcaatgtg gaaaggccat
     9481 tcccaccccc aatagcgaca acccgcgggc cgcgctcctt gcgccgatgc tgggtgatcg
     9541 catcaacaac atgccgtcca ggtcttagga agggtcgcag gatagtgcga ttgaggccat
     9601 agataccggt gataataagc cccaacccca acccaccaaa aattatcgcc cgcagcggtc
     9661 gagcgataaa tcgcaatgaa gccgtcgata acagtggcaa ccaccacgta tcgggggttg
     9721 tccggtaaat atccagaatg atgattgcca ggcctacgcc aattaacgtc gttcctgcca
     9781 gtattaccag caaccagcgt ttgaccccca atccgggaac aagccatcgc aaacttcgcg
     9841 ccgctgcgtt gagccagcgg cggtagagag gagcactatc tttctccata tcatcaagga
     9901 tgatagcagt gttttcctaa ctcgtcaaca gtggggcaag ctttatgcta tactttcgaa
     9961 tatgagtgtg catatcgccg tggatattgg gggcacacat atgcgggctg catattatcc
    10021 cgctcacagc cacgagcccc tcgaacacaa gaagatcact accaagcatc ccagcgagtc
    10081 ggcctgggag cgcctggtga aattgatcac ctccatctgg cccgcgggag aggaagtggc
    10141 tagcattggc gtggcagcac ccggtccatt ggacccattt agcggagttt tattctctgc
    10201 tccgaatatc catgaatgga tcaatctgcc cttacgcaag ttgcttgagg atcaatttga
    10261 agttcctatt gcagtgggta atgatgccaa ccttgccgcc cttggcgaat ggaagtttgg
    10321 tgcagggata ggtcataaac accttgtata tttgacaatc agcacaggga taggaggtgg
    10381 ggttatcatt gataatcgcc tgttagtagg aacgcgcggc ctaaccgccg aatttggaca
    10441 tgtcagtgtc caacccgatg ggccactttg cggatgcggt cagcgcggcc acttggaagc
    10501 actggcagcg ggacccgcca ttgctcgttg ggtggagaca gaaattgctc agggtgtacc
    10561 atctagtctg gcaaagcttg cgcaattcac aaccaaacag gttgctgaag ccgctaacca
    10621 aggagacgac ctggccattc gcgccctgaa ccgcgcaggc aacttcattg ggcgggcgct
    10681 ggcagacata ctgcatatct ttaacccatc tatcattatt atcggtggtg gcgtctcgcg
    10741 cagtggcgac ctcctcatgc acccgattcg aaaggaaatg gaagcgcgcg taatgaaccc
    10801 gcattatctt gacaacctga ccctgaccac tgccgcccta ggagatgaga cggggctact
    10861 aggagcatta gctcttgcgc gggatattaa ataatggaaa aacgctggat aatccctcca
    10921 cgactaccat tggaagtcga tcatgcattg catggttatc cgcctatatt gcggcaaatc
    10981 ctatacaatc gcggttacgc aacccacgaa gatgctcgca gcttccttga agcgagaacc
    11041 cctcctggca ccgaccccct caatctctca ggcgtagccc cagcagttga gcgaatccaa
    11101 tcggcgatcc gccatgctga accaatagcc atatatggtg attacgacgc agatggcgtt
    11161 accgctacag ccttgttggt ggaagtatta aatcgcctgg gagctgatgt gcgcggctac
    11221 atccccaatc gctttgatga aggttatgga ttaaataacg aggccctcag cagtcttaga
    11281 gaccagggca ttcgcctcat catcacagtg gattgcggca ttcgctccgt atctgaagca
    11341 gaacatgccc gccaattggg tatagatttg atcatcagcg atcatcacca ccccgggccg
    11401 gagatccctc atgcttttgc ggtgatcaat ccgaaattac cgggagatac ttttccagaa
    11461 aaggatctag ccggtgtagg actagcatac aaattagctt gcgccttgat aggtgatgac
    11521 caagctgaat caagcactga tctgctcgac ctggttgcct tgggcacagt agctgacatg
    11581 gctccgttgg ttggtgagaa ccgcgccctc gtgcgcgccg ggcttgaagt attgcgacga
    11641 cctcgccgtc aagggattcg gtcattgatg ggagcctctg gcctgatgcc agataaaatc
    11701 aacgcgtccc acatcggctt tgtgctcgga cccaggttga acgctgctgg tcgcctggat
    11761 accgccctgg ctgccttaaa tcttttatta acatccgatt tgggtgaggc tggcatatta
    11821 tcacagcaac tggatatgta caatcgcgaa cgccaggaaa ttacccgact tatccaggag
    11881 tctgctgaac aaatggtact cgccgatgac ccacaagcat tgttgcttgt ggctgcccac
    11941 tctgatttta acgctggcgt ggtggggctt gcagcctcaa ggctttgcga gaagtactac
    12001 cgaccatcga ttattgccac acgtggagaa gagtttacac gcgcctcgtg tcggagtatc
    12061 ccggaatttc acattaccca ggcgcttgac caatgtgccg atttgttgga acatcatggc
    12121 ggtcatgcgg cggcggctgg ttttacagtg gtcaacgaga atgtggacga attaatcgca
    12181 cgtttgagat tgatcgccaa tcaagaacta ggtgaactgg acttgcgccc tgttctgcaa
    12241 agcgacatgg agctgcctct taacgaactc aaaccggaat tgcttaatta cctggattgg
    12301 cttcaaccaa ctggttatgg caaccgagcg gtccattttg tgtctcgtaa tttgagggtg
    12361 acacgctcgc aggcggtcgg cagagatggc tcccatttga aaatgtcggt gagcgatgga
    12421 cgcatcacct atgacgctat tgcgtttaga cagggtcact ggttagaaga tttacccact
    12481 cacgtggatc tgctgtatga atttgaaacg aacgaataca acggtcgggt ttcgcttcag
    12541 ttaaacgtgc gggatattaa accgtccagc taactccgtg gcaaaaagct cgccatgcta
    12601 taattcaaag gttacatttg attgaggagc ataaaatatg accgctttcc gtcccgcaaa
    12661 tcctgccttc agcacgagca aaccatcagg tggtcatttc gtccctccac cgctctcact
    12721 aattgaagat accggcctgt ctgctctctg gttgcaagat cttgctctaa aaatcatgta
    12781 tttccagggt tatatgacgg gcttcaagat ggctgaggag attgccttgc ctttcaatgg
    12841 cattgtagac cagatactcg aatctctaaa aagggagaaa ttcgtcgagg taaagtcatc
    12901 tcaacaaatg gggttgggtg aagggtctta tcaatatgct ctcactgggg ctgggattgc
    12961 ccgcgctcgt gaggccatgg agcgcagcca gtacgccggc cctgctccgg ttcctttgaa
    13021 agtgtataac gaatcgatct tacgttatag caaaggccgg cctatggttg gcagccgcga
    13081 ccttaggcaa gcgctgtctg agctggtgct caacgagcgc acataccagc gcttaggccc
    13141 agcgatcaat tctggcactt cgatcttcct ctacggcccc cctggcaatg gtaaaactac
    13201 gatcgctcgt gctgtaggcc aaatgatttt gcgcgagagc atgtatattc cctatgcact
    13261 atatgtcgat ggacaagtgg tcaagctttt tgattcggtc aatcatcagc tactacgtga
    13321 tgaagattcc accccattag gcactggttc attgcgtagc ggctcacgac gagatccacg
    13381 ctggctacgg attcgacgcc catttatcgt tgtcggcggc gagcttaata tggaaagctt
    13441 ggatctggtt tttgatgatg tgaataaatt ctatgaagcg cccttccagg ttaaagccac
    13501 aggcggtatt ttattaattg atgactttgg caggcagcaa attagacctc gcgatttact
    13561 caaccgctgg atcgtgccat tggaaaatcg catcgactat ttgaccttgc acacgggtcg
    13621 caaggtcgaa ataccctttg atgtactagt gattttttca accaacctgc ccccgaaaga
    13681 cctggtagac gaagccttcc tacgtcgctt gcggcataaa attgaaattg gtgacccaaa
    13741 ctacaacgat tatcgggaga tcttccggag agtggcagta gccaaggaca ttacctatag
    13801 tgatcgcggg ctggcttact tgcttcaaga gtggtatatc aagcgagggc gtaagatgcg
    13861 cgcctcccat ccgcgtgacc tgtgcgatca aattctggat atcgcccgtt atttatcggt
    13921 tgaaccggcg atgacacgcg atcttctcga ccgggcggca gaatcgtatt ttgttgaact
    13981 gtattaaaaa ttcaacctat gttggaccaa cttcccaatc ctgccattat cgctcacagg
    14041 ggcgctagcg cctacgcgcc tgaaaatacg cttgcagcat tcgagctagc tgtgcgccaa
    14101 aaggctgatg cgattgaact cgatgccaaa ctcacgctcg acaaccatgt ggtagtattt
    14161 catgaccaaa ccctggagcg aacgaccggc gttcatggac gggttattgg ctccaaatta
    14221 gctgatttac gccgcctaga tgcaggcagc cacttcgatg tggcttattg gggtgagacg
    14281 atcccgacct tggacgaggt tttcg
//
LOCUS       DBNGSX010000096        15068 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1392233, whole genome shotgun sequence.
ACCESSION   DBNGSX010000096 DBNGSX010000000
VERSION     DBNGSX010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 15068)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15068)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15068
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1392233"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1121
                     /locus_tag="AC16CB_03170"
     CDS             135..1121
                     /locus_tag="AC16CB_03170"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3341500.1"
                     /translation="MSKVRWGLLSTANINRRLIPAIRASQRGELVAVASRSKATSAAY
                     AKKWDIPHAFGSYEGMLNSGMVDAVYISLPNHLHAEWSIRAMQAGVHVLCEKPFAISL
                     EEVDAMVAASHQYGRALAEAFMYRHHPQTKIAGEWAHSGRLGKICLVRSIFNFPLQNR
                     GNVRLVPEYGGGCLWDVGVYPLSFSQYILGRLPEWVIGAQWIGASGVDETFAGQMHYS
                     GDQMAQIASSFRSPQYTYAEIIGTEGQLILNRPFIGLGDPERQLMFYPVKGEAQEIPV
                     PEQELYVGEVEDMHAAILDNQPCYIKLEESRSHVQTALALYEAAQKGKSVSV"
     gene            1209..1997
                     /gene="pstB"
                     /locus_tag="AC16CB_03175"
     CDS             1209..1997
                     /gene="pstB"
                     /locus_tag="AC16CB_03175"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIR3341501.1"
                     /translation="MNILIPDGNISLEVRDLKVWYGTTPALKDLDLQIPSRKITAIIG
                     PSGCGKSTFIRCFNRMNDLIPGARVGGDVLYHGKNIYSSDIDPVEVRRSIGMVFQKPN
                     PFPKSIYDNIAYGPRISGYKGNMDDLVEKALRNAALWEEVKDKLKTSGLALSGGQQQR
                     LCIARAIAVEPEVILMDEPASALDPIATLKIEELMQQLKENYTIVIVTHNMQQAARSS
                     DYTAMMMLTEDMKSGTVIEFGRTTDIFTHPKDNRTEDYVTGRFG"
     gene            2028..2708
                     /gene="phoU"
                     /locus_tag="AC16CB_03180"
     CDS             2028..2708
                     /gene="phoU"
                     /locus_tag="AC16CB_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIR3341502.1"
                     /translation="MIRELLDRKLQHLLDELLVLDGMVEQAIIESVDALKQQDLALAR
                     KVYDGDIKVNAKRFEIENECMITIATQQPIMAGDLRLLASILEVVGELERMGDYAKGI
                     AKICLMVGNEPHVKPLIDIPRMAEIAVSMLHRAVGAFINKDTETAKLIPQEDDMVDGL
                     YNQIYRELVAIMLTNPTTIDRANYLMWAAHNLERVADRVTNVCERTIYVTTGELKEID
                     VSDDESKV"
     gene            2712..3164
                     /locus_tag="AC16CB_03185"
     CDS             2712..3164
                     /locus_tag="AC16CB_03185"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HIR3341503.1"
                     /translation="MGKKTILFLCTGNSCRSQMAEAIVNARLGERWHAYSAGTEPTGY
                     VHPKALATLAEIGINHQGHSKSLDTFRNTRFDLVVTVCDSAAEECPYWPGSGRTVHLG
                     FPDPAKVSGDDEQVMAVFRAVRDQIAEKMTELFGQSVPSNLQPYIPVQ"
     gene            3390..3749
                     /locus_tag="AC16CB_03190"
     CDS             3390..3749
                     /locus_tag="AC16CB_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341504.1"
                     /translation="MKIIAYIAAAILIFFGVLFIWGAFSPEGSVGWIPVGLISVGIGF
                     GLIWFASRKKPQVEASSQNITLKIDLPGDVNLETLKCQSCGGTLTMDNIEMVAGAPVV
                     NCPYCKTTYQLTEEPKW"
     gene            3751..4332
                     /locus_tag="AC16CB_03195"
     CDS             3751..4332
                     /locus_tag="AC16CB_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341505.1"
                     /translation="MKKKIFPFLLLCLFALLPGSALAQTYYFSMDQETVHVFWNEDGT
                     TSIDYVFVFTNDNSASPIDFVDVGLPNSNFDKSTIFADVNGKELTNISASDYQGGGDS
                     GVAVALGSYAIRPGQTGQVHVFVGTIRRMLRPDSDDKEYASGVFSPTWFGRQYVYGST
                     NLTVTYHFPPGVNPKSHAGMPHQAAGLQSQSQA"
     gene            4479..5408
                     /locus_tag="AC16CB_03200"
     CDS             4479..5408
                     /locus_tag="AC16CB_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341506.1"
                     /translation="MDDLLGLFFCLGFIGIFGFSIVASIRSVSRRKLQYLPPKIAIEG
                     HGIKRGLTAIEAAILMEQPLDKILTMILFAVIKKGAASVTVRDPLKLDIVSPLPEDLH
                     GYETKFLEAFSKSPSARRSDLQNMMVDLVKTVSTKMKGFSRKETLAYYRDIVQRAWGQ
                     VETADTPEVKSQKFDEVMEWTMLDRDYDGRTRRVFTGPVFVPIWWGRYDPTYTRGPIS
                     APTPTSGGGGISLPHLPGSDFAASIVNGVQNFSGNVIGNVTDFTSKVTNKTNPAPVST
                     SSGRSFTGGGGGGGCACACACAGCACACAGGGR"
     gene            5527..6837
                     /locus_tag="AC16CB_03205"
     CDS             5527..6837
                     /locus_tag="AC16CB_03205"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3341507.1"
                     /translation="MNSKPAHNLSQPGLYHFTRQKRFERSRIHLRIDPDGSGLLLVNA
                     SRVMHLNPTATYMAHLYLEDIPEAQALRAITKTYHVSLDQAKADFRQIATQLDELIRP
                     DGVCPIHELEIDMIPPFSARPSAPYRMDLALTYRCNNDCAHCYNARSRSYPELTTEQW
                     KQILDRLWELGIPHVVFTGGEPTLRSDLPELVAHAEHNGQITGLNTNGRRLKDPHFLK
                     LLVEAGLDHVQITLESHNPKIHDIMVQKNGAWNQTVAGLRNALDSPLFVMTNTTMLQE
                     NSPTLAETLDFLGELGVPTIGLNALIYSGHGLTVGTGLRERDLQPLLELARQRTEIFA
                     QRLIWYTPTQYCNFDPMQLELGVKGCTAALYNMCVEPDGAVLPCQSYYQSLGNLLIDP
                     WDKIWSHDLAVSLRERRSIPSKCNDCALLAECGGGCPLMFNLNN"
     gene            6849..7997
                     /locus_tag="AC16CB_03210"
     CDS             6849..7997
                     /locus_tag="AC16CB_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HIR3341508.1"
                     /translation="MTSLFAPIRRLFDPIKPIPQGIYHYQSPLEDPRNYRLHLRIEED
                     GSGIMIVNASTVLHLNQTAAEYAYYFVKNTPAEKVTQLVSQRYSVDPAQAAKDYHNLT
                     ERILTLIEVPDLDPVTFLDFERKTPYARTILAPYRLDCALTYRLPKGADPEAAPKKRV
                     DRELNTQEWISVLDKAWHAGIPHIVFTGGEPTLREDTLTLIAHAEHNGQVSGLLSDGL
                     RLVEEAYLVDLLQTGLDHLMMVLEPENDLSWQALGKVLAADLFTAIHLTLTPQNAASL
                     PGYIKKLAEMGTPAISLTSQDPSLGETLQALRDHAADLQMSLVWDLPVPYSSQNPIAM
                     ELVEEKLVDGAGRAWLYVEPDGDVLPAQGLNQILGNILNDPFEKIWNR"
     gene            complement(7994..8638)
                     /locus_tag="AC16CB_03215"
     CDS             complement(7994..8638)
                     /locus_tag="AC16CB_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012797.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR3341509.1"
                     /translation="MDTHGIQVRKCVRAECGMRFPLLLSDQHGKRCPECGASTRIVLE
                     KIVAPESGGELKTYHNIKIEAMLDNIRSAWNVGSMFRTADGVGIRYMHLCGLTPTPDN
                     PKVTKTSLGAEVSVPWLSYRDGVRTAGELKGKGMRLWALEHNPGAESMLELQDIPADT
                     SIVLVVGNEIAGVDPGILELCERVMYLPMIGLKRSYNAAVAFGIAAHYLRFAVP"
     gene            8666..9433
                     /locus_tag="AC16CB_03220"
     CDS             8666..9433
                     /locus_tag="AC16CB_03220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3341510.1"
                     /translation="MSANETPINWHLRFAQQARWTEDVRRFLFTRAAIDSARRVLDVG
                     CGTGVLLDELEGNFDTALYGIDIASNHLKMAIRNTQRSFFTQSDAHALPYPSQSFDLA
                     YCHFLLLWVSEPWKVIQEMARVVRPGGAVLALAEPDYGGRIDYPSELEELGQAQHASL
                     AIQGADPLMGRKLSEIFHQAGLTSIETGVLGGNWTSSPLLEEWQDEWRVVEHDLQQTS
                     ELIPNPEVLKKFDWVAWQAGKRVLFVPTFYAFGLVPL"
     gene            complement(9435..9785)
                     /locus_tag="AC16CB_03225"
     CDS             complement(9435..9785)
                     /locus_tag="AC16CB_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HIR3341511.1"
                     /translation="MYNYKNLKPEECDFVAVASEDDIPPGERLFVEIDQFPIVVFNLA
                     GQFYAIADLCSHDDGPLGDGNVEGWEVACPRHGARFDIRTGKVLSLPAVEDIPAYPVL
                     VLDGEIQVGIPLED"
     gene            complement(9856..10251)
                     /gene="sufU"
                     /locus_tag="AC16CB_03230"
     CDS             complement(9856..10251)
                     /gene="sufU"
                     /locus_tag="AC16CB_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HIR3341512.1"
                     /translation="MDDFYRELIIDRYKNPQFRGLLDPHDISFEDENPLCGDHIRIDV
                     RVDGNGTVTEAAFDGHGCAISQASADLLLESIVGKPLEDIKEMNREDILDLLGIELGP
                     VRLKCALLSLKVLKAGVYGLGEASDDLVE"
     gene            complement(10252..11499)
                     /locus_tag="AC16CB_03235"
     CDS             complement(10252..11499)
                     /locus_tag="AC16CB_03235"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - obsolete cysteine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HIR3341513.1"
                     /translation="MTIHTDNKLNVQRIRQDFPILTREVRPGVPLVYLDSTATSQKPQ
                     VVIEAMDNFYRRSNANIHRGIHTLAEESTAAYEAARQQVADFIHAPSARQVVFTRNTT
                     ESINLVAQSWGRANMNPGDVIILTEMEHHSNLVPWQILAAEKNLRLEFIPVTPDGLLD
                     LETYGELLRLEPKLVSFTHMSNVLGTINPAEEIVQLAHQSGAVTLIDAAQSVPHFSVD
                     VQELDADFLAFSAHKMCGPSGIGALYGKKALLEAMPPFLGGGDMIKRVELRSFVPNSL
                     PHKFEAGTPAIAEAVGFAAAVDYLSKVGMDAIAAHEHEIVEYALERLEEVPGVSMFGP
                     AAQFRGGVAAFTLAGVHAHDVAQVLDTDGIAVRAGHHCAMPLHNKFNLPATARASFYL
                     YNTRQEVDMLAESLYKVKTIFRV"
     gene            complement(12008..13366)
                     /gene="sufD"
                     /locus_tag="AC16CB_03240"
     CDS             complement(12008..13366)
                     /gene="sufD"
                     /locus_tag="AC16CB_03240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HIR3341514.1"
                     /translation="MTTRKVVSRTRHAERQISREFPFGQEMIPALAGEPHLPQFLVDY
                     RSRAWQAFNSLPLPSLTDEPWRRTDLRGLNAGSFRLSQPETGGHLTEVPDEYLQPVAA
                     EDHGGQIVLLPGGNSIIELHPDLAAKGVVFTDLVTAQRQYPEILRRILGQVVRPEEGK
                     FAALAAAFAQSGVLLYVPRGVQVDLPLQSLFWGPGSGLAHVSHILVLVDDGASLTYVH
                     ESASQTEPSGQTMHAGIVEIHVGAGANLRFVELQSWGEHVWNFTHERARVDRDGNLDW
                     IFGAVGTQLTKNFSDLDLIGQGAVGRMSGFYFTDGIQHLDHDTQQNHLAPNTTSDLLF
                     KGALKDRSRSVWQGMIYVAPGAQKTDGYQANRNLTLSPKARADSIPGLEILADDVRCT
                     HGATVGKIDPEQTFYLRSRGIPTIEAEKLIVEGFFEPIMQRIPFEGVRGRFQSAIQEK
                     LV"
     gene            complement(13368..14774)
                     /gene="sufB"
                     /locus_tag="AC16CB_03245"
     CDS             complement(13368..14774)
                     /gene="sufB"
                     /locus_tag="AC16CB_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HIR3341515.1"
                     /translation="MSSDAVILDGIGEYKYGFSDPETYVFKSRKGIDREVVEQISSMK
                     GEPEWMLAFRLKALDHFLKRPTPTWGGDLSTLNLDDIYYYVKPADSESKTWDDVPEAI
                     KNTFDKLGIPEAERKFLAGVGAQYDSEMVYHKILEHLEAQGVIFLSIEDGLRKHPDLF
                     REYFSTVIPIEDNKFAALNSAVWSGGSFIYVPKGVKVDLPLQAYFRLNVANIGQFERT
                     LIIADEGAQLHYVEGCTAPIYTTDSFHSGVIEIVVKKGARVRYTTIQNWSVNVYNLVT
                     QRALVYEGATMEWVDANLGSKLTMKYPSCYLLGPGAHGEILSMAFAGKGQHQDAGGKV
                     IHFAPHTSSKVTSKSISKSGGRASYRGLLKVHKEAEDVRSNVVCDALLLDETSRSDTY
                     PYIEIDAEDVTIGHEASVSKVGEEQLFYLMSRGLSEEEATTMVVSGFIEPLVKELPME
                     YAIEMNRLIQLQMEGSIG"
     gene            complement(14787..>15068)
                     /gene="sufC"
                     /locus_tag="AC16CB_03250"
     CDS             complement(14787..>15068)
                     /gene="sufC"
                     /locus_tag="AC16CB_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358469.1"
                     /note="part of SUF system involved in inserting
                     iron-sulfur clusters into proteins; in Escherichia coli
                     this protein forms a complex with SufBD; the SufBCD
                     complex stimulates the cysteine desulfurase SufS in
                     conjunction with SufE; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HIR3341516.1"
                     /translation="LRPEIAILDETDSGLDIDALRIVSEGVNALRSEELGVLVITHYQ
                     RILNYIKPDYVHIMLGGRVVESGGPDLALHLEEHGYDWIREKFESEPVS"
BASE COUNT         3894 a         3950 c         3539 g         3685 t
ORIGIN      
        1 gtatggctat aaaaaagggg gtggtcatgt tacactattc atgtctttga attgcctcca
       61 cccaccgata gaaaccagtt tctgtgccat ggggaaggct atttaatcct gaaattgaga
      121 ttacaggagg aacgatgtcg aaagttcgct ggggattact ctcgactgcc aatatcaatc
      181 gacgcttgat ccctgccatc cgagcctcac agcgaggtga gctggtagct gttgccagcc
      241 gctcaaaggc aacttcagca gcctatgcaa aaaagtggga cattcctcac gcatttggaa
      301 gctatgaagg catgctcaac tccggcatgg tcgatgcggt ctatatcagc ttaccaaacc
      361 atttgcatgc cgagtggagc atccgcgcaa tgcaagctgg agtacatgtg ctctgcgaaa
      421 agccttttgc catctcattg gaggaggtgg atgccatggt cgccgccagc catcaatatg
      481 gacgggcgct ggctgaagct ttcatgtacc gccaccatcc gcagacaaag attgccggtg
      541 aatgggcgca cagcgggcgg ctgggaaaga tttgtctggt gcgcagcatt tttaacttcc
      601 ccctgcaaaa tagagggaac gtgcggctgg tgccagagta cggaggtgga tgcctgtggg
      661 atgtaggagt ttatccgctg agtttctccc aatatatact cgggcgtctt ccagagtggg
      721 taataggggc acaatggata ggtgcaagtg gtgtagacga aacctttgcc ggccagatgc
      781 attattctgg tgaccaaatg gctcaaatcg catcgtcttt tcgttcccct caatatacgt
      841 acgctgaaat catcggcact gagggtcaat tgatactaaa tcgacccttt atcggtcttg
      901 gagatccaga aaggcagtta atgttttatc cagtaaaagg cgaagcccaa gaaatccctg
      961 tcccagaaca ggaattatat gtcggcgaag tagaggatat gcacgctgcc atcttggata
     1021 atcaaccatg ttatattaaa cttgaggaat cacgcagcca cgttcagact gccttggctc
     1081 tctatgaggc tgctcaaaaa ggaaaaagtg tttcagtata aaaactctgc gttgatgcct
     1141 gtgtgttggt gatataaatt atccgccatc attttgcgca atcgactagc ataccaggtg
     1201 atatttacat gaacatttta atacccgatg gaaacatctc cctggaggtt cgcgacctaa
     1261 aagtatggta tgggaccaca cccgcattaa aagatcttga ccttcaaata cctagccgaa
     1321 aaattactgc tattatcgga ccatctggct gcggtaagag cacttttatc cgctgcttca
     1381 accggatgaa tgacttgatt ccaggcgccc gggtgggtgg ggatgtactc taccatggta
     1441 aaaatattta ttcatccgat attgaccctg tcgaagtacg tcggagtatt ggaatggtat
     1501 tccaaaaacc caatcctttc cccaaatcaa tctatgataa tatcgcctat ggtccacgga
     1561 tcagtggtta caagggaaat atggatgatt tagtggaaaa agccttacgc aacgcagcgc
     1621 tctgggaaga agtaaaagac aaactcaaga catctggcct ggctctctcg ggcggtcagc
     1681 aacagcgtct ttgcatcgcc cgggcaattg ctgtcgagcc tgaggttatt ttgatggatg
     1741 aaccggcatc ggctttagat ccaattgcca ctttaaagat agaggagttg atgcagcaac
     1801 tcaaagagaa ttatacgatt gtgattgtca cccacaatat gcagcaagcc gcccgctctt
     1861 ccgactatac cgccatgatg atgctcaccg aagatatgaa atcggggacg gtgattgagt
     1921 ttgggcggac gactgatata ttcacccatc ctaaagacaa tcgtacagaa gattatgtaa
     1981 ctgggcggtt tggataggag agagaatcca gataaacggg gtagaacatg atcagagaac
     2041 tgttagatag aaaacttcag caccttctgg acgagcttct ggtcctcgat ggcatggtcg
     2101 aacaggctat catagagtct gtcgatgcat tgaaacaaca agacctggct ctagccagaa
     2161 aagtatacga tggcgacatc aaggtaaacg caaaacgttt tgaaattgag aatgagtgca
     2221 tgattaccat cgccacgcaa cagcccatta tggctggtga tctccgtttg ctggcttcaa
     2281 ttctggaggt cgtcggagag ctcgaacgta tgggcgatta tgcaaaaggc attgctaaaa
     2341 tctgtttgat ggtgggcaat gagccccatg taaaaccgct catcgatatc ccgcgcatgg
     2401 ccgaaatcgc ggtcagcatg ctgcatcgtg ctgtgggtgc gtttatcaat aaggacacag
     2461 agaccgccaa gctcattcca caagaggatg atatggttga tggtctctac aaccagatct
     2521 atcgagaact ggtagccatt atgttgacca atccaaccac catagaccgc gctaattacc
     2581 tgatgtgggc cgcgcacaac ctggagcgag tagcagaccg cgtgaccaat gtatgcgaac
     2641 gtacgatcta cgtaaccaca ggcgaactca aagaaatcga tgtatccgac gacgagagta
     2701 aagtataaac tttgggtaag aaaacaatcc tctttttatg cacaggtaat tcctgtcgct
     2761 cccaaatggc tgaggcaatc gtcaatgctc gcctgggcga acgctggcat gcttatagcg
     2821 ccggcaccga accaacggga tatgtacacc ctaaagccct ggctaccttg gctgagatcg
     2881 gaattaatca ccaagggcac tcaaaaagcc tagatacttt ccgcaacacg aggttcgatc
     2941 tggtagtaac cgtttgtgat tcagccgcag aagaatgccc atactggcct ggatcaggtc
     3001 gaaccgtcca ccttggcttc cctgatccag caaaggttag tggagacgat gaacaggtca
     3061 tggctgtctt tcgcgctgta cgtgatcaga tcgctgaaaa gatgaccgag ttgtttggac
     3121 aatctgttcc atcaaactta cagccatata tcccagttca ataatttaag ccgaggcaaa
     3181 agaaacccac aataatatcc attcacaccc tgattcgata aaagcaaagc caggacaacg
     3241 cattttcgat aggatatacg cggattctgg cttttattat ccgagttgtg acaaaataag
     3301 gaaatttatc atttgcccga attcaattaa attattaaac ttataggaac aatccacgta
     3361 tataggacat agacaggagc aaattttcaa tgaaaattat cgcctatatc gccgcagcca
     3421 tattaatctt ttttggagtt ctttttatct ggggggcttt cagcccagaa ggctctgtcg
     3481 gttggatacc tgtcgggcta attagcgtag gaattggctt tggcttgatc tggtttgcca
     3541 gccgaaaaaa acctcaggtc gaggcaagct cccaaaatat caccctcaag atcgatttgc
     3601 caggtgatgt caacctggag accctcaagt gccaatcctg cggcggcacg cttacaatgg
     3661 ataacattga aatggtagcc ggtgctccag tagtaaattg cccttattgc aagactacct
     3721 atcagctaac cgaggagccg aagtggtaac atgaagaaaa aaatatttcc tttccttctt
     3781 ctctgtctgt ttgccctctt accgggcagc gccttggcac aaacctacta cttcagcatg
     3841 gaccaagaga ccgtccatgt tttctggaat gaagacggga caacctcgat agattatgta
     3901 ttcgtcttca ccaatgataa ttctgcatca cccattgatt tcgttgatgt tggtttacct
     3961 aactcaaatt ttgataaaag cacaatcttc gccgatgtga atggcaaaga attgaccaac
     4021 atctccgcca gcgattacca gggtggaggg gatagcggcg tagcagtcgc cttaggcagc
     4081 tacgcaatta gaccgggaca gactgggcaa gtgcatgtct ttgtgggtac tatccgccgt
     4141 atgctcagac cagacagcga tgacaaggaa tacgcgagtg gcgtgttctc accaacctgg
     4201 tttgggcgtc aatatgtcta tggttcgaca aatttaaccg ttacctacca ttttccgccc
     4261 ggtgtaaacc cgaagagcca cgctggcatg cctcaccaag cggctggtct tcagagccaa
     4321 tcacaggcgt agacaatgaa gggcgaattt attacacctg gacgaacccc aatgccaatg
     4381 gctatacgca gtatcttttt ggagcttctt tcccgataca atacgttcct gactcagcta
     4441 tcgttaaacc caatattttc gatctactcg gaatctccgt ggatgacctc ctaggactgt
     4501 ttttctgttt gggttttatc ggaatattcg gtttcagcat cgtcgcttcc atccgcagcg
     4561 tttccaggcg aaagctgcaa tatctgcccc ctaaaatcgc catcgaaggc catggcatca
     4621 agcgcggatt gacagccatt gaagccgcaa tcctcatgga gcagcccctg gacaaaatcc
     4681 taaccatgat cttgttcgca gtgattaaaa aaggcgccgc ttctgtgacc gtgcgcgatc
     4741 ctttgaaact cgatatcgta agccccctcc cggaggattt gcacggctat gaaacaaaat
     4801 ttctcgaggc gttctcaaaa agcccatccg cgcgccgctc agacttacaa aatatgatgg
     4861 tcgatttagt taaaactgtc tcaaccaaaa tgaaggggtt tagccgaaaa gaaaccctgg
     4921 cttactaccg cgacattgtc cagcgcgcct gggggcaggt agaaaccgcc gacacacctg
     4981 aagtaaagag tcagaaattt gatgaggtca tggaatggac catgctggat cgagattacg
     5041 acggccgcac gcggcgggtt ttcaccggtc cagtattcgt tccgatctgg tggggccggt
     5101 acgatcccac gtatacacgc gggccaatta gtgcgcccac tcccacttct ggaggtggcg
     5161 gaatttcgct gccccatcta cccggctcgg atttcgctgc ttcgatcgtt aatggtgtgc
     5221 aaaatttctc tggcaacgtg atcggcaacg tgaccgattt taccagtaag gtaacgaaca
     5281 agaccaatcc cgcgccagtt tccaccagca gcggtcggag cttcaccggc ggaggtggcg
     5341 ggggtggctg cgcttgtgcc tgtgcctgcg ccggttgtgc ctgcgcctgc gcgggtggtg
     5401 ggagatagcc tgaaattaga gaacataaga taatccaggt ttatgaagtg caatcaaatg
     5461 gtgcgtatga cggaaatccg atctaaaatc aagtcgttag gtcagatgtt taatattcag
     5521 accctaatga actcaaaacc ggcgcataat ctctcgcaac caggtcttta tcatttcact
     5581 cgccaaaagc gtttcgagcg cagccgcatc cacctgcgaa ttgacccaga tggcagtggt
     5641 ctgttgctgg tcaatgctag ccgggtgatg catctcaacc ccaccgcaac gtacatggca
     5701 catttgtact tagaagatat tcctgaagct caggcgttac gcgccatcac gaagacctat
     5761 catgtctctc tcgaccaggc aaaagctgat ttcaggcaaa tcgccacaca attagatgaa
     5821 ctaatccgtc cggatggcgt ctgcccaatc cacgagttgg aaatcgacat gataccgcca
     5881 ttcagcgctc gcccttcggc gccatatcgc atggaccttg cattgaccta ccgttgcaat
     5941 aacgactgtg cccactgcta taacgcccga tcgaggtctt atcccgagct gacgactgaa
     6001 cagtggaaac aaatccttga tcgcttgtgg gagctaggca ttccgcatgt agtctttacc
     6061 gggggtgagc ccacgttgcg cagcgatctg ccagaattgg tcgcccacgc cgagcacaat
     6121 gggcagatca ctggtctaaa taccaacggg cggcgactaa aagacccgca ctttctaaag
     6181 ttattagttg aagctggctt agatcatgtc cagataacgc ttgaatcaca taacccaaaa
     6241 attcatgaca taatggtgca aaagaatggc gcgtggaatc aaaccgtcgc aggactacgc
     6301 aacgccctcg attctccgct gttcgtcatg accaatacta ctatgctcca agagaacagc
     6361 ccaaccttgg cggagacgct cgattttctt ggtgagctgg gcgtccctac cattgggctc
     6421 aatgcattga tctattcggg tcatggcctc acggtgggaa ccggattgag agaaagagat
     6481 ttgcaaccgc tcctcgaact ggcacgccag cgaaccgaga tattcgccca acgcttgatc
     6541 tggtacacac ccacccaata ttgcaatttc gacccgatgc agcttgaact gggcgtcaaa
     6601 gggtgcactg cagcgctcta caacatgtgc gtcgagccag atggcgcggt attgccttgc
     6661 caatcttatt atcagtccct gggtaattta ctgattgatc cgtgggacaa gatttggtct
     6721 catgatttag cggtgtcatt gcgcgagcgc cgcagcatcc cgtcaaaatg caatgactgc
     6781 gcccttcttg ctgagtgcgg aggcggttgc ccgcttatgt tcaacctgaa taattgagga
     6841 aaaaatccat gacatcctta ttcgccccaa ttcgccgcct gttcgacccg atcaaaccga
     6901 taccccaagg tatttaccat taccaatcac cgctcgaaga cccgcggaat tatcgcctac
     6961 acttgcgcat cgaagaagat ggcagcggca ttatgatcgt taacgcctcc accgtgttgc
     7021 acctcaacca aactgcggct gaatacgctt actatttcgt caagaacacg cccgccgaga
     7081 aagttaccca actggtctca cagcgttaca gtgttgatcc tgcgcaagct gctaaagact
     7141 accacaacct caccgaacgg atcctgaccc tgatagaggt acctgacctc gacccggtca
     7201 cattcctgga tttcgagcgg aagacaccct acgcacgcac gattttggct ccctaccggc
     7261 tggactgcgc ccttacatac cgcctaccaa agggcgctga ccccgaggct gctcctaaaa
     7321 agcgcgtaga tcgagaactg aacacgcagg aatggatttc agtcctggat aaagcgtggc
     7381 atgcgggcat tccacatatc gtatttactg gcggtgagcc cactttgcga gaagacactc
     7441 tgacattgat tgcccatgcg gagcataatg ggcaggttag cggcttgcta tccgatggtc
     7501 tgcgccttgt tgaagaggct tacttggtcg atctgctcca gactggttta gaccacctga
     7561 tgatggtcct ggaacccgag aatgatcttt cctggcaagc tttggggaag gttctcgcgg
     7621 ccgatctctt tacagcaatc cacctgacac tcacacctca aaatgccgca tctcttcctg
     7681 gatacataaa gaagcttgcc gagatgggca cccctgcaat ctccctcact tctcaagatc
     7741 caagcctggg agagacgctt caagcgttgc gggatcacgc tgccgatttg caaatgtcgc
     7801 ttgtatggga tttaccagtg ccttactcaa gccagaatcc aattgctatg gagctggttg
     7861 aagagaaact ggtggatggc gctggacgcg cttggcttta cgttgagcct gatggcgatg
     7921 tgctccccgc tcaaggcctc aaccagatcc tgggcaacat actaaacgac cccttcgaga
     7981 aaatctggaa tcgctaaggc acagcaaagc gcagataatg cgctgcaatt ccaaaagcca
     8041 ctgccgcgtt ataggaacgc tttaaaccta tcatcgggag atacatcacc cgctcacaaa
     8101 gctccaagat cccagggtcc actcccgcaa tttcgttccc cactaccagc acaatcgagg
     8161 tgtctgctgg aatgtcttgc agctcaagca tcgattccgc acctggatta tgttcgagag
     8221 cccagaggcg catgcccttc cctttaagct cgccggctgt gcgtacgcca tctcgatagg
     8281 ataaccaggg tacgcttact tctgcaccca gcgaagtctt cgttacctta ggattatccg
     8341 gcgtcggggt caatccacac agatgcatat accggatgcc aaccccatct gccgtgcgga
     8401 acattgagcc gacattccat gcactgcgga tattatccag catcgcctca atttttatgt
     8461 tgtggtaagt ttttaattcc ccaccagatt cgggcgcgac tattttttcc agcacgatgc
     8521 gcgtcgatgc cccacattcg ggacagcgct tcccatgttg atcgcttagg agcaggggaa
     8581 agcgcatccc gcattcagct cgaacacact tacgcacctg aattccatgg gtatccatag
     8641 gcacattcca ttataatcac tttatatgtc tgcaaatgaa acaccaataa attggcacct
     8701 gcgttttgca cagcaggcac gctggacaga ggatgtccgc cgtttcctgt tcacacgggc
     8761 ggctatcgat agtgctcgtc gtgtactgga cgtgggctgc ggcacaggtg tgcttttgga
     8821 tgagttggaa gggaacttcg atacggcatt atatggcatt gatatcgcat caaaccacct
     8881 aaagatggct atacgcaata cgcaaagatc attttttact caaagtgatg ctcacgcgct
     8941 tccctaccct tctcaatcat ttgatctggc atactgccat tttttgcttt tatgggtcag
     9001 tgaaccctgg aaagtaattc aagagatggc ccgtgtagta cgacctggcg gcgcagtgtt
     9061 agccttagcc gagccagact atggcgggcg catcgattac ccatccgagt tggaagaact
     9121 gggacaggca caacacgctt ccttagcaat tcagggcgct gatcccctta tgggtcgcaa
     9181 gctgagcgag atttttcatc aagctggcct cacatccatt gagactggag tgctcggtgg
     9241 aaattggact tcttcgcctt tgctagaaga atggcaagac gaatggcgcg tggtcgagca
     9301 tgatttacaa caaacctccg aacttattcc aaatccggag gtcttgaaaa aattcgattg
     9361 ggttgcctgg caggctggca agcgggtatt atttgtgcct actttttatg ccttcggcct
     9421 ggtgcccttg tgatctaatc ctccaagggg atgcctacct gtatctcccc atcgagaacc
     9481 agcaccgggt aagccgggat atcctccaca gccggcagag aaagcacctt tccagtacgt
     9541 atatcaaatc gagcaccgtg gcgcgggcag gcgacttccc agccctcaac attgccgtcc
     9601 cctagcggac catcatcgtg agaacacagg tctgcaatgg cataaaactg acctgctagg
     9661 ttaaacacca cgattggaaa ctgatcaatc tcgacaaaca gacgctcgcc cggcgggata
     9721 tcatcctcac tggcaactgc aacaaaatca cactcttccg gcttcaaatt cttatagttg
     9781 tacatcgaag tcatatgctt tttgggaaat tttattattt atattggcta tgcgaaaata
     9841 atcagagaaa agaaatcatt cgaccaaatc gtcactggct tcgcccaatc catatacccc
     9901 ggccttcagc acctttaatg ataataacgc gcatttcaat cgtaccggcc ccaactctat
     9961 ccccagaaga tccaaaatat cttccctatt catttctttg atatcttcga gcggtttacc
    10021 aacgatcgat tcaagcaaca gatctgcaga tgcttgagat atggcgcacc catgcccatc
    10081 aaaagcggct tccgtcactg ttccattccc atccacgcgc acatcgatgc gaatgtgatc
    10141 gccacacaga gggttttcat cctcaaatga tatgtcgtgt gggtctagca agccgcgaaa
    10201 ttgggggttc ttatagcgat caatgatgag ttcgcggtaa aaatcgtcca tttacaccct
    10261 gaaaatcgtt tttactttat ataaggactc agccaacata tccacttctt ggcgggtgtt
    10321 gtagaggtag aagctggcac gcgcagtcgc tggcaaattg aatttattat gcaatggcat
    10381 agcacagtga tgacctgcgc gcacagcaat gccatccgta tccaaaacct gcgcgacatc
    10441 gtgagcatgt actccagcca gtgtaaatgc tgccacacct ccccggaatt gagcagcagg
    10501 accgaacatg cttacacctg ggacctcttc caatcgttcc agggcatact caacaatttc
    10561 atgctcatgc gcagcgatgg catccatacc cactttcgag agataatcca cggccgctgc
    10621 aaagcccaca gcctcggcga tagcaggtgt gcctgcctca aatttatgcg gcaacgagtt
    10681 ggggacaaac gagcgcaact cgacgcgctt tatcatatcg ccacctccta gaaaaggtgg
    10741 catagcctct aaaagcgctt tcttgccata aagcgcacca attccgcttg gcccgcacat
    10801 cttatgcgct gagaaggcta agaaatctgc atcaagctcc tgtacatcca ctgaaaaatg
    10861 aggcaccgat tgcgccgcat cgatcaaggt cactgcaccg gattgatggg caagttgaac
    10921 gatctcttca gccggattga tggtccctaa aacattagac atgtgtgtaa aagagaccaa
    10981 cttcggctcc agcctcagta actcaccgta ggtttccaga tcgagtaatc cgtctggggt
    11041 taccgggata aactccaatc gcagattttt ttcagcggcc agaatttgcc agggtaccag
    11101 gttggagtgg tgctccatct cggtcaagat aatcacatcg cctgggttca tgttggcacg
    11161 cccccagctc tgagcaacta aattgatcga ttcggttgta ttacgcgtaa agacaacctg
    11221 ccgagcagat ggcgcatgga taaaatcggc tacttgctgc cgggcagcct cataggctgc
    11281 cgtagattcc tctgcaagtg tatgaatacc tcgatggata ttagcatttg aacgccgata
    11341 aaaattatcc atggcttcga tcaccacctg cggtttttgg ctggtggctg tcgaatccag
    11401 gtataccagc gggacaccag gacggacctc cctggtcaag attgggaaat cctggcgaat
    11461 tctctgcaca ttcagcttat tgtcggtatg gatggtcatg atcgtgcgat ccaaaagata
    11521 agatagcgct gaatacctat ctgacccgca cgcgcggacg cctcttgcgc cgcgcaagtc
    11581 ggacattact agaatttttc gggcaccata aaacgtgatc cggtaccatc cacccatcgg
    11641 tcaggtaaac attttcgcga aattgaacgg ctatcaactt cgtgtccact tggacgacag
    11701 taccgaccag ccaatcacgg acccgagtac catttttgtc gtgatcacac agcacctcaa
    11761 cctgatcgcc gacattatat ttttctactc cactaggagt tgttttgaac tttaactctg
    11821 ccaactccgc ctcctgattt aatatttcaa acaccagagg caagctaatg attttatgct
    11881 cgccatgttg ttgccattac ccctccactc gcctgtgatg cttttttatt caattagtat
    11941 tcatctcata atcaacggaa acccgatgat tacagcactc actatttaga agatgtctat
    12001 taatattcta tactaatttt tcctgaatcg cgctctggaa gcgccctcga acaccctcaa
    12061 atggaatgcg ttgcatgatt ggctcaaaga aaccctctac aattagcttc tcggcttcga
    12121 ttgtgggtat accacggctg cgtagataga aggtctgctc agggtcaatc ttgccgactg
    12181 tagcaccatg tgtgcagcgc acatcgtccg ccaatatctc caacccgggg attgaatcgg
    12241 cacgcgcttt cgggctgagt gtcaaattcc ggtttgcttg atagccatcg gtcttctgtg
    12301 cgcccggggc tacatagatc atcccttgcc acactgacct gctgcgatct ttcagcgcac
    12361 ccttaaagag taaatcgctg gttgtgttgg gggcgagatg gttctgctga gtatcatggt
    12421 cgaggtgttg gataccgtct gtaaagtaaa aaccagacat ccgcccaaca gccccttgcc
    12481 caatcaaatc cagatcggaa aaattctttg tcagttgagt gccaactgcg ccgaagatcc
    12541 aatccaggtt gccatctcgg tcgacgcggg cgcgttcgtg ggtaaaattc caaacgtgct
    12601 ctccccagga ttgtaattcc acaaatcgca agtttgcccc tgcccccaca tggatctcga
    12661 caattccagc atgcattgtt tgaccactgg gctcagtttg cgaggctgat tcgtgcacgt
    12721 aagtcaatga ggcgccgtca tcgaccaaaa caaggatatg agaaacatgg gctaagccgc
    12781 taccaggtcc ccaaaacaaa ctctgcaatg gcaaatcaac ctgtacacca cgcggcacat
    12841 acaacaaaac acctgactgc gcaaaggcag ccgctagagc cgcaaatttt ccttcttctg
    12901 gacgtacaac ttgacccaaa attcttctta atatttcagg atattgccgt tgggcagtca
    12961 ccaagtcggt gaaaaccact ccctttgcag ccaaatcagg atgcaactca attattgagt
    13021 taccacccgg caataatact atttgtccgc catgatcctc cgcagcgaca ggctgtagat
    13081 attcatcggg gacctcggtc aaatgcccgc ctgtttcggg ttgagaaagg cgaaaagagc
    13141 ctgcattcag tccgcgaaga tcagttctcc gccagggttc atctgtcaga ctaggcagtg
    13201 gtaagctatt aaaagcttgc catgcccgtg agcggtaatc aacgagaaat tggggaagat
    13261 gaggttctcc ggcaagcgca ggtatcatct cctgtccaaa aggaaattca cgagaaattt
    13321 ggcgttctgc gtgtcgagtt cggcttacta ctttgcgtgt cgtcatatca gccaatggat
    13381 ccttccattt gcaattggat caagcggttc atctcaatag catattccat gggcaattcc
    13441 ttgacaagcg gttcaatgaa gccagacacc accatagttg tggcttcttc ctcgctcagc
    13501 ccacggctca tgagataaaa cagttgctct tcgccaacct tagataccga agcctcatgt
    13561 ccaatggtga catcttcggc atcaatttca atgtatggat atgtatcaga cctggacgtc
    13621 tcgtccagca ggagcgcgtc gcagacaaca ttcgagcgca catcttcggc ttctttatgc
    13681 actttaagca agccgcgata agaagcgcgc ccgccacttt tgctaatcga tttcgaggtg
    13741 actttgctgc tggtatgcgg cgcgaaatgg atcactttgc cgcctgcgtc ctggtgctgt
    13801 cccttacctg caaatgccat agacaggatc tcgccatgcg ccccaggccc cagcaggtag
    13861 caagaggggt atttcattgt cagcttggag cccaggtttg catctaccca ttccatggtc
    13921 gcaccttcat acaccagagc tcgttgcgta accaggttat acacattcac tgaccaattt
    13981 tggatagtgg tgtaacgcac gcgtgctcct tttttgacca caatctcgat cacacctgaa
    14041 tgaaacgaat cggtagtata gatgggcgct gtgcagcctt ctacgtagtg cagctgtgcg
    14101 ccctcatcgg caataatcaa ggtgcgctcg aattgtccaa tgttggcaac attcaagcgg
    14161 aagtatgcct gcaagggcag atccactttg acaccctttg ggacatagat gaacgatccg
    14221 ccagaccaga ccgcgctatt aagcgcagcg aacttgttat cttcgatggg tatgaccgtg
    14281 ctgaaatact cgcggaacag gtcggggtgc ttgcgtaggc catcctcaat gctcaaaaag
    14341 atcacaccct gagcttccag atgctccaat atcttgtggt aaaccatctc agagtcatat
    14401 tgcgccccta cgcccgccag gaatttgcgt tctgcctctg gaatgccaag cttatcgaag
    14461 gtgtttttaa tggcttcggg aacatcgtcc caggttttcg attcagaatc cgctggtttc
    14521 acataataat aaatatcatc caggttcaga gtggagagat ctcctcccca agtgggtgta
    14581 ggtcgcttca ggaaatggtc cagcgccttg agacggaatg ccagcatcca ctccggttca
    14641 cccttcatac tcgagatctg ttcaacaact tcgcgatcaa tacctttacg ggatttaaat
    14701 acataagtct ccggatcgct aaatccgtat ttatactcgc caataccatc caaaattacc
    14761 gcatcactac tcattgaatt cctccgttaa gaaaccggct ctgactcgaa cttctcccta
    14821 atccagtcgt atccgtgttc ttcaagatga agcgctaaat caggaccacc agattccaca
    14881 acccgccccc ccaacatgat gtggacatag tccggcttga tatagttcaa aatacgctgg
    14941 tagtgtgtaa ttacgagcac accaagctcc tcgctgcgca atgcattgac tccctcagag
    15001 acaatccgca acgcatcgat atccaagcct gaatctgtct catccaaaat agcgatctcg
    15061 gggcgtaa
//
LOCUS       DBNGSX010000097        11626 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1392354, whole genome shotgun sequence.
ACCESSION   DBNGSX010000097 DBNGSX010000000
VERSION     DBNGSX010000097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11626)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11626)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11626
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1392354"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1558)
                     /locus_tag="AC16CB_03255"
     CDS             complement(<1..1558)
                     /locus_tag="AC16CB_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR3341517.1"
                     /translation="MSEPKTLEVPILGMDCADCTLHVKRAISALPGVEAVEVFLASEK
                     AIVSLDPRLVDMPAIRKAVASAGYAVPEMEEGAKTSSLQDFTRPILTLLGVIFGVVLF
                     VVVVGEWLGMFEAITKRIPWPVGLAAVLLAGFPVFRNVARAALKRQVIAHTLMSVGVV
                     AALAVGEWATAVVVVFFMRVGDYAEHFTTDRARRAVRDLAELAPQTARLERDGEEIEV
                     PIDQVAPGDLLVVRPGEKIPVDGEVIAGRATVNQSMITGEGLPVEVGPGSRVYAASLA
                     SLGAVKVRADGVGVDSTFGRVIKLVEQAEASRAEVQRIADRFSAYYLPVVAGVAAITF
                     LLRRDPLATAAVLVVACSCSFALATPIAMLASIGAGAKRGLMIKGGKYLESLAQADVL
                     LIDKTGTLTLGKPEIVDFRLLMGDSSDQSTISNQQSQILMYAASAERYSEHPLAEAVR
                     QAARQRELSLYEPVDFQALPGKGVQARVDGHWIEVGREAAGQVTNSPYHELETQGKTP
                     LFVKIDGELVG"
     gene            1658..2821
                     /locus_tag="AC16CB_03260"
     CDS             1658..2821
                     /locus_tag="AC16CB_03260"
                     /inference="COORDINATES: protein motif:HMM:NF013381.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR3341518.1"
                     /translation="MSMTSRERVQTALHHEEPDRVPIIIGASNATSMKLKPYLGLKDL
                     LGIQGEDRYLYDWPELGSIHPDERILQRLLSDVRGVLDRAPQAIYHRNRKRPAHTPYI
                     DDWGIGQEEIEPDVWFPSLHPMAAARSLDDIENYPWPDMHDPTRVAHVRQVAKRLSQE
                     NQYAIMATPWLLFPFERANALMGMEHFLPNLVENREFAVALLLKITELCKTLMGHFLS
                     ELGKDVDIIKIGDDLGMQSGLLISPRMYRQVLKPIHADYIAFIKAHTKAKVFFHTDGD
                     VFDLIDDFIEIGVDILNPIQTSAGKMADLVAIKKRYGRNLCLCGAVDTYRILPFGTPE
                     QVRQEVRRVIDILGPGGGYMVAAVHTILDEVPAENILAMVDAVEAFGHYPLGN"
     gene            2923..3687
                     /locus_tag="AC16CB_03265"
     CDS             2923..3687
                     /locus_tag="AC16CB_03265"
                     /inference="COORDINATES: protein motif:HMM:NF015722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HIR3341519.1"
                     /translation="MSGRSASLLAIALLGLAGCSRPTPTPTIPKSDLVQVPAGWFWMG
                     EDDARRSSQPQHRVYLDTFEIQRTEVTRSDYIQSLAAIGYSAPDTDQSKLEASSQLPV
                     VGVLWEHADAYCRWIGMRLPTEAEWEKAARGDNGRHFPWGNEWDPNKANTSENGQGNV
                     LPVGSFLDGASPYGLLDMCGNAAEWVADYYQASYYHDAPDRNPTGPKEVTDHVLRGGS
                     FDSTYEQTTTYFRDSSHSVRPNLRVGFRCARSVETK"
     gene            complement(3735..4895)
                     /locus_tag="AC16CB_03270"
     CDS             complement(3735..4895)
                     /locus_tag="AC16CB_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3341520.1"
                     /translation="MKFVTNGSVMAPLPIVIQPASDLPLAFQLKQQITWLVATNVIKP
                     GEKLPPIRVLAAQVGVHYQTVRAVYHQLSEDGLVAIRQGSGATVLPFSGLQLARPETS
                     VHAKMVGVLIAGMDPFYLPFLQGVEEVAEALPSLVVICNAEDNPVKAKLQINQLILRG
                     MDGIIAASVGNLVQRELGFPQTGGPFPIVYCDQPDTSGHVILFDAENAGYQAVKHLLD
                     HGHRRIGIVTAPIDWPNMSECFAGYRRALMEAGIAPRQSLVSVTKGFSISQGSDAARS
                     LLVGSNPPTAMFATADILAIGAQRAIKSEGLRVPEDVAMIGYNDIEIASMVEPALTSL
                     AAPAYEMGRLAMGLLARLIAGERVNPTITRLETSLVIRRSCGCYPPGEGDHH"
     gene            4992..7061
                     /locus_tag="AC16CB_03275"
     CDS             4992..7061
                     /locus_tag="AC16CB_03275"
                     /inference="COORDINATES: protein motif:HMM:NF042769.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341521.1"
                     /translation="MRRISAITRTAVVMMLFTWLSACSSQPPTPAKSPPTHTDIPVEP
                     TAAPVTPEARAVATQTLPEPTAPPSWDYVAIGGDFTYAQNWDKLYAGYMEDDLEVPVN
                     FSDLSTHDFMTWNGWLERLKNEEQLRSALQGAEAVTFDVPLEAYFSMPIAMYQNKQCG
                     GDDGENCYRQRVEQLRQDLDAFLAELTALADPSQVVVRTVLRGSIPAFYGEGVGEGFV
                     QDFSEAEMQALIKYMLAANEVVRQATAAHGIPAVDLPATLQPDGPDTPPASEYLLMLG
                     LSEAGDQEVADLLRQAGYESSPQAEVTLREEGCSLGVYANPVPNPIQVKVANPTNEIM
                     ALVVVTLKEDASRYDLLEFTGGGIPDEVDKRVAHPEPGPNNEMITSITLEEGRENYFV
                     CAQPEKVLAVPGALTPGQAYQPFPPVRAVREVPYTKPVSATAQEFKLDIYAPKQAGLY
                     PVVVFLHGGNTSKDDPVYQELFTDLVMAGAVVLSAQSFENSGGALARADKGAGIRHDH
                     ENAACAVRYARAVAPQYSGDPKQVILIGHSGGGYTGLWVTLAGDQLDQAWETVASQGG
                     PPRQVECLAGQDESPLPDGFIGYNGGLVYFDQMRNEFPSLFKVLDPRSHVGRNPEIPL
                     LLVFGDQDFSYVYMPWFKKGVDEMVSMLEKAGHEVQFELWPGIGHSVDSRSIPLIVSA
                     FQRMVRR"
     gene            complement(7074..8354)
                     /locus_tag="AC16CB_03280"
     CDS             complement(7074..8354)
                     /locus_tag="AC16CB_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3341522.1"
                     /translation="MAATTLRREIEFVSPTRQMVMGILFVIIGAGIWFFFSQGIQPDV
                     QTKFSLTPGGSTAVLPDWIVPTAMTSNVLSGLSILLGLFQIVRKGGFRRYTNWVLGGV
                     AAMFIFAFLTWAAAGKSLNLAGMINTTLIKAVPLTLGAMSGILCERSGVVNIAIEGMM
                     LMGAMVGALVASIADNLWIGLLAAVASAALLGLVHAVLSIKYKTDQIISGTAINIFST
                     GMTSYISAKFMQVYQTELNNPGVFLPIEIPLLSKIPILGTILFRQNIFFYAMFVFLVL
                     LSFGLFKTRWGLRLRSVGEHPRAADTLGINVFRTRYMAVLLGSMVAGFGGAYFTIGSV
                     GGFDEVMTAGRGFISLAAMLFGNYTPFGSFGAGMLFGFADSLASKLSILGVKIPSEFL
                     LMVPYVATMVVLAGVVGRSRMPAADGKPYEKEGH"
     gene            complement(8358..9653)
                     /locus_tag="AC16CB_03285"
     CDS             complement(8358..9653)
                     /locus_tag="AC16CB_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3341523.1"
                     /translation="MSKAQPQSPEGPPKKDAGKIFLEELIGMRPHPGSRWRRILQSIL
                     VPALAIFTGLVLGALVILFTSPEFFAAFKVSLWEGLKTGWKVVSITYGALFVGAFGSP
                     SAIFSALLSGETAAIREAFYPFLESLVASTPYIFGGLSVALGFRVGLFNIGGEGQLFL
                     GAIFATYVGYSLTGLPVFLHMPIAFLAGALGGAVWGFIPGILKAKTGAHEVINTIMLN
                     YVAFRLSEWLLNGPMQRPDSFNPVSPFIQESAKLPRFFAPPIRFHMGFFIALLVAWLV
                     YWFLWKTKWGFSLRMVGSNPHAARYAGASVVMGTVMGMTLAGALAGLAGANEVLGLNY
                     YLAMAFSSGYGFDSIAIALLGNTQPLGVVLSALLFGALRNGATLMQLKGGIPIDIVSI
                     VQAMVLVFVAAPAIIRSVYRLRAPAPGEEAGLATSWGGD"
     gene            complement(9646..11202)
                     /locus_tag="AC16CB_03290"
     CDS             complement(9646..11202)
                     /locus_tag="AC16CB_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3341524.1"
                     /translation="MATVLELKGITKRFPGVLANDHIDLTLEEGSIHALLGENGAGKT
                     TLMNILYGLYQPDEGEIFVRGKPIVVHSPSDAIAAGVGMVHQHFMLVPVFTVTENVML
                     GEEALRYGDVLDRKSAAKKIRQISEQYSLEVDPDAYVEDLPVGVQQRVEIIKLLYREA
                     EILILDEPSAVLTPQEVEELFKIMRSLVAQGKSIIFITHKLKEVLEIADQITVLRRGK
                     VIGTTTPADADQHVLAEMMVGREVQLVVDKESIKLGDVVLDVQDLVVLNQRHQMAVND
                     VSFKVSAGEVLGIAGVQGNGQTELIEALTGLRAVQSGRIQIDGTEVTHATPRKITEQG
                     VAHVPEDRHRDGLVLAFPVSDNLMLNTYYLPPFSKGVVLQESEIVRSADKRIQEFDIR
                     TPGALTSTSSLSGGNQQKVIVAREFSRPIKLLIAAQPTRGLDVGSIEYIHNRLIQKRD
                     EGCAILLMSTELDEILGLSDRIAVMYRGRIVETLKASEATKEGLGLLMAGMRPVSQAA
                     INSIGGADNV"
     gene            complement(11410..>11626)
                     /locus_tag="AC16CB_03295"
     CDS             complement(11410..>11626)
                     /locus_tag="AC16CB_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3341525.1"
                     /translation="VLKNMDITTQAAIEQAYNDSFEGGSIVGTLENGGVGLAPFHDAD
                     ALVPADLKAELDQVKADIIAGTIQTSP"
BASE COUNT         2745 a         3415 c         3141 g         2325 t
ORIGIN      
        1 ctccaaccag ctcgccgtcg attttgacga atagaggcgt ttttccctgg gtttctagct
       61 cgtggtaggg cgagttggtt acttgccctg cggcttctct gccgacctcg atccagtgac
      121 catccacgcg cgcttgcacg cccttgccag ggagagcctg aaaatccacc ggttcgtaca
      181 agctcaattc acgctggcga gctgcctggc gcactgcctc agccagtggg tgttcggaat
      241 agcgctcagc ggaggcggcg tacatgagaa tttgagattg ttgattgctg attgttgatt
      301 ggtcagacga atcacccatc agcaatctaa agtctacaat ttccggtttg cctagcgtca
      361 gggtacccgt cttatcaatg agcagcacat ctgcctgcgc cagactttcc aggtacttac
      421 ctcctttgat catcaggccg cgtttagccc ccgcgccgat agaggccagc atggcgatgg
      481 gtgtcgccag ggcaaaagag caggagcacg ccactaccag cacggcagcc gttgccagcg
      541 gatcgcggcg caacaagaaa gtgatcgcag ccacacccgc cactaccggc aggtaatagg
      601 ctgagaaacg gtcagcgatg cgctgtactt cagcgcggga agcctcagcc tgctccacga
      661 gcttgatcac acgtccgaag gtggagtcta ctccaacgcc atcagcgcgc accttcacgg
      721 cacccaggct ggccagcgag gctgcgtaga ctcggctccc cggccccact tcaacaggca
      781 gcccctcgcc ggtaatcatg gattggttca ccgtcgcccg gccggcgatt acttcaccgt
      841 cgactgggat tttctcaccc ggcctgacca ccagcagatc gccgggagct acctgatcga
      901 taggcacttc gatctcctcg ccatcacgct ccagccgcgc cgtctgcggc gcaagctcag
      961 ccagatcgcg caccgctcgg cgggcgcgat cggtggtgaa gtgctcagcg taatcgccta
     1021 cacgcatgaa aaaaaccacc acaacagcag tagcccactc acctaccgcc agggcagcaa
     1081 ccacgcccac cgacatcagc gtatgggcga tcacctgacg cttcagggca gcccgtgcca
     1141 cattgcggaa caccgggaaa cctgccagca gcaccgccgc cagccccaca ggccagggta
     1201 ttcgtttcgt gatcgcctcg aacatcccca gccactcgcc gaccactacc acgaacagca
     1261 ccaccccaaa gataaccccc agcaaggtca ggataggtcg ggtaaaatct tgcaaggatg
     1321 aggttttcgc cccttcctcc atctcgggga cggcataccc tgcactcgca acagctttgc
     1381 ggatggcagg catatccacc agccgcggat caagactgac gatggctttt tcagaagcca
     1441 aaaaaacctc caccgcctcc acacccggta atgcagaaat ggcacgcttg acgtgcagcg
     1501 tgcaatcggc gcagtccatg ccgaggatag ggacttcgag tgtcttgggt tcggacatat
     1561 ggacagatta tattacaatc agagttaaga atttaatcct gctcgatcac gatcaactct
     1621 gcgtgctttg taaatctact aggctgaaga atttaatatg agcatgactt cacgagagag
     1681 ggttcagact gctctccacc acgaagagcc ggaccgcgtg ccgatcatta tcggagctag
     1741 caatgctact tcgatgaagc taaaacccta ccttgggcta aaagacctgc ttggtatcca
     1801 gggtgaagac cgctatctat atgattggcc agagctcggc tcgattcacc ctgacgagcg
     1861 catcttacaa cgcctgctca gcgacgtgcg cggcgtgctg gatcgcgccc cgcaagccat
     1921 atatcatcgc aatcgaaagc gtcctgcaca cacgccctac atcgacgatt ggggcattgg
     1981 gcaagaggag atcgagcccg acgtctggtt tcccagtctg caccccatgg cggcagccag
     2041 aagcctggac gatatcgaaa actacccctg gccagacatg cacgatccaa cacgcgtggc
     2101 acatgtccgc caggtcgcca agcgcctgtc acaagagaat caatacgcca tcatggctac
     2161 accctggctg ctcttcccat tcgagcgcgc caacgcattg atgggtatgg aacacttctt
     2221 accgaacctg gtggaaaacc gcgagtttgc tgtggcactg cttcttaaga tcactgagct
     2281 atgcaaaacg ctcatggggc attttctgag cgagcttggc aaggacgttg atatcatcaa
     2341 gatcggtgac gacctgggta tgcagagtgg gctactcatc tcgccgcgca tgtatcgcca
     2401 ggtgctcaaa ccgatacacg ccgattatat tgctttcatc aaagcacaca cgaaggccaa
     2461 agtatttttt cacacggatg gtgatgtgtt tgacctcatc gatgatttca tcgagatcgg
     2521 tgtagacatc ctcaatccca tccagacttc ggcaggtaag atggctgatc tggtggcaat
     2581 caagaaacgc tacggcagga atctatgctt gtgcggggcg gttgacacgt accgcatcct
     2641 gcctttcggc acgcctgaac aggtgcgcca ggaagtgcgg cgggtgattg acatcctggg
     2701 accggggggc ggctacatgg ttgctgcggt acacactatc ctggatgagg tgcctgccga
     2761 gaacatcctg gctatggttg acgcggtaga agcgttcggg cattatccac tgggaaatta
     2821 aatctcacca ccaagaacac aaaggaacac gaaaggaaaa ggattattta accgtatgtt
     2881 taacattcgt gtcctttgtg tcagaaagtt atccgtatga atatgtcggg aagatccgcc
     2941 agcttgctcg caatcgccct gctgggactg gcaggctgca gtcgtccaac ccctacgcca
     3001 accataccaa aatccgacct ggttcaggtg cctgctggct ggttctggat gggcgaggac
     3061 gatgccagaa ggtcgagcca accccagcat cgcgtctatc tggatacgtt tgaaattcag
     3121 cgcactgagg tcacccgtag cgattatatc cagtctctcg cagccatcgg ttactcagcg
     3181 cccgacacgg accaatctaa acttgaggca tccagccagc tgcccgtggt tggtgtccta
     3241 tgggaacatg ccgacgcata ctgccgctgg atcggcatgc gcctgcccac agaggccgag
     3301 tgggaaaaag ccgcccgtgg cgacaacggg cggcatttcc cctgggggaa cgagtgggat
     3361 cctaataaag ccaatacgtc agagaacggt cagggcaacg tactgccagt aggcagcttt
     3421 ctggacggtg ccagccccta tgggctgctc gatatgtgcg ggaacgcggc cgaatgggta
     3481 gcagactatt atcaagcgtc ttattaccac gatgcaccag atcgcaaccc aaccggtccc
     3541 aaagaagtga cagatcatgt actgcgcggc ggctcgtttg actctaccta cgagcaaacc
     3601 accacctact ttcgtgactc gtcccactcc gtccggccaa acctgcgggt tggtttccgc
     3661 tgtgcgcgtt ctgttgagac caaatgaggt tatcgttttt tggcaagaat ggggtggtca
     3721 ggaatcgcaa gagcctaatg atggtcccct tccccaggtg gatagcagcc acaactgcgt
     3781 cggataacta gactggtctc caacctcgtg atagtaggat taacgcgctc tccagcgata
     3841 agtctggcaa gtaaccccat tgccaggcgg cccatctcat aggctggggc ggctaggctg
     3901 gtcaacgctg gttccaccat agacgcgatc tcgatatcgt tataaccgat catagcaaca
     3961 tcttctggca cgcgcaatcc ctcggatttg atggcgcgtt gagcgccaat tgccaggata
     4021 tcagcggtgg cgaacatggc ggttggagga ttggagccta ccagcaatga gcgagccgca
     4081 tcgctcccct gtgaaatcga gaaacctttc gtgactgaca ccagagattg acgcggcgca
     4141 atgccagctt ccattaaggc tcggcgatag ccagcaaagc attcgctcat attcggccaa
     4201 tctatggggg cagtgacgat accaatacgg cggtgaccat ggtccagcag gtgttttacc
     4261 gcctgatatc cagcgttttc agcgtcgaac aggatgacat gaccgctcgt atctggctga
     4321 tcgcagtaga cgattggaaa aggaccgcct gtctgaggaa atccaagctc tctttgcaca
     4381 agattcccaa ccgaggcagc aataatgcca tccataccgc gcagaatcaa ctggttgatc
     4441 tgcagcttgg ctttaaccgg attatcttct gcgttgcaaa tcacgaccag ggatggtaat
     4501 gcttcagcga cctcctcaac cccttgcaga aagggcaaat agaagggatc cattccagcg
     4561 atcaatacgc cgaccatctt tgcatgcacg ctggtttccg gcctagctag ctgcaggccg
     4621 gagaatggca gcacggtagc acccgatccc tggcggatgg ccaccaagcc gtcttccgag
     4681 agctggtgat agactgcccg cacggtttgg taatgcacac ccacctgggc tgctagcacc
     4741 cgaatgggtg ggagcttttc gccaggtttg atcacgttag tggcgaccag ccaggtgatt
     4801 tgctgtttga gctggaacgc caggggtaaa tctgaggctg gttggatgac aatggggaga
     4861 ggagccatca ccgaaccgtt tgtgacaaat ttcattaatt ctacctgctt gacctgaaat
     4921 aaatcaatgt gttataatgg attataacac attgatcgta ccggcctgct gatccaacct
     4981 aggagggact gatgcgccgt atatccgcaa tcacccgcac tgccgttgtc atgatgctct
     5041 tcacttggct gagcgcgtgc agcagccagc cgcccacccc agcaaagtcc ccccccaccc
     5101 acaccgatat acccgtggag cccaccgcgg caccagtcac cccggaagcg cgtgccgtag
     5161 ccacccagac tctgccggag ccaaccgcgc ctccatcatg ggactatgtc gccatcggcg
     5221 gcgatttcac ctacgcgcag aactgggaca agctgtacgc cggttacatg gaagatgacc
     5281 tagaagtgcc ggtcaacttt tccgacctgt ccacccacga cttcatgacc tggaacggct
     5341 ggctggagcg gctgaaaaac gaagaacagc tgcgcagcgc cctgcagggg gcggaggcgg
     5401 tcacttttga tgtgcccctg gaggcctatt tctcgatgcc catcgccatg taccagaaca
     5461 agcagtgcgg cggcgatgac ggggagaact gctaccggca gcgcgtcgag caactgcgcc
     5521 aggatctgga tgcctttctg gcggagctga ctgcgctggc cgatcccagc caggtggtgg
     5581 tgcgcacggt gctgcgcggt tcgatccccg ctttctacgg cgagggggtc ggcgaaggct
     5641 tcgtgcagga tttcagcgag gcggagatgc aggcgctgat caagtacatg ctggctgcca
     5701 atgaggtggt gcgtcaggcg acagctgcgc acggcatccc tgcggttgac ctgccggcca
     5761 ccctgcagcc ggacgggccg gacacccctc cggcgtcgga atatctgctc atgcttggcc
     5821 tgtccgaggc cggggaccag gaggtggctg atctgctgcg ccaggcgggc tacgaatcct
     5881 caccccaggc ggaggtgacc ttaagggagg agggctgcag cctgggggta tacgccaacc
     5941 cggtgccgaa tccgatccag gtgaaggtcg ccaacccgac caatgagata atggcgctgg
     6001 tggtggtaac gttgaaagag gacgccagcc gctacgatct gctcgaattc actggcggcg
     6061 ggatcccgga tgaagttgat aagcgcgtcg cccacccgga gcccggcccg aacaacgaga
     6121 tgattaccag catcaccctg gaagaggggc gtgagaatta cttcgtctgc gcccagccgg
     6181 aaaaagtgct ggcggtgccg ggggcgctga cccccgggca ggcctaccaa cccttcccgc
     6241 cggtgcgcgc ggtaagggaa gtgccctaca ccaagcctgt ctcagccact gcgcaggaat
     6301 ttaagctgga catctacgcc ccgaaacagg caggattata cccggtggtg gtcttcctac
     6361 acggtggcaa cacctccaaa gacgacccgg tttaccagga gctgttcaca gaccttgtga
     6421 tggcaggagc agtggtgctc tctgcccagt catttgagaa ttcaggcggg gccctcgccc
     6481 gcgcagataa gggtgctggc atccgtcatg accacgaaaa cgcggcatgc gcggttcgct
     6541 atgcccgcgc cgttgcgccg caatacagcg gcgaccctaa gcaggttatc ctgatcggcc
     6601 actccggggg aggctacacc ggtttgtggg tcaccctggc aggcgaccag ctcgaccagg
     6661 cctgggaaac tgttgcaagc cagggcgggc caccccgcca ggtggaatgc ctagcagggc
     6721 aggatgaaag ccctctacca gatggcttca tcggatacaa cggtggcctt gtctatttcg
     6781 accagatgag aaatgaattt ccctccttat tcaaggtact agacccgcgc agccatgtgg
     6841 gtcgcaatcc tgaaatacct ctcctgctgg tgttcggcga ccaggatttc agttatgtat
     6901 atatgccctg gttcaaaaaa ggggtggacg agatggtatc catgctcgag aaagccggcc
     6961 atgaagtcca gttcgagttg tggccgggca tcggtcactc tgtcgatagc cgtagcatac
     7021 cactcatcgt gtctgcgttc cagaggatgg tgaggcgcta ggcaatcaaa tccttagtgc
     7081 ccttctttct catacggctt gccatctgca gctggcatac ggctgcgccc gaccacgccc
     7141 gccagcacga ccatcgtggc gacatagggt accatcagca agaactcgga tgggatcttg
     7201 acccccaaga tggagagctt ggaggctagc gagtcggcaa agccaaataa catcccagcc
     7261 ccgaaagagc caaagggggt gtagttgcca aagagcatcg ccgccagaga gataaaaccg
     7321 cgcccagcgg tcatgacctc atcgaagccg cccaccgatc caattgtgaa gtaggcgccg
     7381 ccaaatccag ctaccatgga tcctaagagc acagccatgt agcgcgtgcg gaagacattg
     7441 atgcccagcg tgtcagcggc gcggggatgt tcacctacac tgcgcaggcg taacccccag
     7501 cgagttttga aaagcccaaa gctaagcagc accaagaata caaacatagc atagaagaag
     7561 atgttctgcc gaaacaggat ggtgcccagg atgggaattt ttgacagcag cgggatctcg
     7621 ataggcaaaa atacacccgg gttattcaat tctgtctggt agacctgcat gaacttggcg
     7681 gaaatatagg acgtcatacc agtcgaaaag atgttgatgg ccgtaccgga gataatctgg
     7741 tcagttttgt atttgatgga cagcacagcg tgcaccaggc cgagcaaagc cgccgaggca
     7801 accgccgcca gcaaaccgat ccataggttg tctgcaatgc tggcgaccag agcgccaacc
     7861 atggcaccca tcaacatcat gccttcgatg gcgatgttca ccaccccgga gcgttcgcat
     7921 aaaatcccgg acatcgcgcc cagggttagc gggacagctt taatcagggt ggtattgatc
     7981 ataccagcca ggttgagcga tttgcccgcc gccgcccagg tcaggaaagc gaagatgaac
     8041 atggctgcca ccccgcctaa aacccagttg gtgtaacggc gaaaaccgcc tttccggaca
     8101 atttggaata accccagcaa gatagacagc cccgatagca cgttcgacgt catagcggtc
     8161 ggcacgatcc aatccgggag cacagcggtt gagcctcctg gggtcaggct gaacttggtc
     8221 tggacatccg gctggatacc ctggctgaaa aagaaccaga tacccgcgcc gatgatgacg
     8281 aagagaatac ccatcaccat ctggcgtgta ggagagacga actcgatctc gcgtcgtaat
     8341 gtcgtagctg ccatggatca atcccctccc caggaagtcg ccagtcccgc ttcctcccca
     8401 ggtgccgggg cacgcaagcg atagactgag cgaatgatgg caggcgcagc cacgaatacc
     8461 agcaccatcg cctgcacaat cgagacaata tcgatgggga tacccccctt gagttgcatc
     8521 agggtcgccc cattacgcaa ggcaccgaac agcaaagccg agagcaccac acccagtggc
     8581 tgcgtattgc ccagcagagc aatagcaatg ctgtcaaaac cataaccgga tgagaatgcc
     8641 atggccagat aataatttaa acccaagact tcatttgcgc cggccaagcc tgccagcgcc
     8701 ccggccagcg tcatgcccat caccgtgccc atgaccacac tcgcccctgc gtagcgggca
     8761 gcatggggat ttgaccccac catgcgcaag gagaagcccc acttggtctt ccacaaaaac
     8821 cagtaaacca gccaggccac cagcagggcg atgaaaaatc ccatgtgaaa acggatgggt
     8881 ggggcgaaga agcgcggcag cttggcactt tcctggataa aaggactaac cgggttgaat
     8941 gaatcagggc gctgcatcgg gccattcaac agccattcgc tcaagcggaa agcgacatag
     9001 ttgagcatga tagtgttgat cacctcatgt gcgccggtct tggccttcaa aatacctggt
     9061 ataaaacccc acaccgcccc acccagcgcg ccagccagga aggcgatcgg catgtgcaag
     9121 aagaccggta gacctgtcag cgagtagcct acataagtgg caaagattgc tcccaaaaag
     9181 agctgcccct caccgccaat gttgaacaag cccacgcgaa atcctaacgc caccgacagg
     9241 ccaccgaaga tgtagggagt cgacgcaacc aggctttcga ggaagggata gaaggcctca
     9301 cggatggcag ccgtctcacc gctgagcaaa gcggagaaga tcgcactcgg gctgccgaag
     9361 gcgcccacaa agagagcgcc gtaggtaatg ctgacgacct tccagccggt tttcaacccc
     9421 tcccaaagag agaccttgaa agctgcaaag aattcagggc tggtgaacaa gatcaccagc
     9481 gctcccagca ccagcccggt aaagattgcc agtgctggga ctaaaatact ttgcagtatg
     9541 cgccgccagc gcgagccggg atgagggcgc ataccaatca gctcttccaa gaaaatcttg
     9601 ccggcgtcct tctttggcgg gccttctggc gattgaggtt gtgctttaga cattgtcagc
     9661 acctcctata gaattaatcg ccgcctggga cacaggtctc atcccagcca ttaggagacc
     9721 taaaccttct ttcgtggctt ccgaggcctt caaagtttcc acaatcctgc cgcgatacat
     9781 cacggcaatc cggtctgaaa gtcccaggat ctcgtccaac tccgtcgaca tcagcaggat
     9841 ggcgcacccc tcatcccgct tttgaatcag gcggttatgg atatattcaa tcgaaccgac
     9901 atccaggccg cgcgtcggct gggcggcaat caacagcttg atgggccggg aaaactcgcg
     9961 cgcaacgatc actttttgct ggttgccacc cgaaagcgag ctggttgatg tcaacgcgcc
    10021 gggtgtgcgg atgtcgaact cctggatcct tttatctgca gacctgacaa tctcactctc
    10081 ctgcagtaca acccccttag aaaatggagg caggtaatag gtattgagca taagattatc
    10141 tgatactgga aatgccagca ccaaaccatc cctgtgccgg tcctccggaa cgtgcgccac
    10201 tccttgctcg gtgattttgc ggggtgtggc gtgggtaact tctgttccat caatctggat
    10261 gcgaccggat tgcaccgccc tcaagccggt caacgcttcg atcagctcag tttggccatt
    10321 accttgcacg cctgcgatcc ccaacacctc ccctgcactc accttgaagg atacatcatt
    10381 cacagccatc tggtggcgct ggttcagcac gaccaggtct tgaacatcca gaactacatc
    10441 cccgagtttg atagattctt tatcgaccac cagctgcacc tcgcggccga ccatcatctc
    10501 agccagcaca tgttggtccg catccgcagg tgtggtcgtg ccaatcactt tcccacgccg
    10561 caggacggtg atttggtcgg caatctccaa cacttctttc aacttgtggg tgataaaaat
    10621 aatcgactta ccctgggcta caagagagcg catgatcttg aaaagctctt caacttcctg
    10681 tggggtgagc actgcgctgg gctcgtcaag gatgaggatc tcagcttctc ggtagagcag
    10741 cttgataatt tccacccgtt gctgtacacc tacaggcaag tcttcaacgt aggcgtcggg
    10801 gtctacctcc aggctgtatt gctcggaaat ctggcggatt ttcttggcag cagacttgcg
    10861 gtccagcaca tctccataac gcagcgcttc ctcccccagc atcacatttt cggtgacggt
    10921 aaacacgggg acgagcataa agtgctggtg aaccatacca acaccagctg caatagcatc
    10981 gctgggagaa tggactacaa taggcttacc acgcacaaag atttcaccct catcgggctg
    11041 gtagagcccg taaagaatgt tcattaatgt ggtcttgcct gccccattct cccccaacag
    11101 ggcgtggata ctcccctctt cgagcgtcag gtcgatatga tcgtttgcaa gcacgcccgg
    11161 aaaacgcttg gtgatccctt tgagttcaag aacggttgcc atgatgacct ttggacctcc
    11221 ggcagagcga tctcaatgtc tttattagat tgaaaagaca gccaataaag gatttgcaca
    11281 aactcctggt taatttaaac ttggcttaag tcattttacc ataaaggaaa gagccggtct
    11341 gtaaccggct cgaaaaaagc aagggggttg gcccccccca accccgtatg ttgtcttttg
    11401 tagcaaaacc tatggcgatg tctggatagt tccagcgatg atatccgcct tgacctgatc
    11461 cagctcagcc ttgagatcag caggcaccag cgcgtccgca tcgtggaacg gagccagacc
    11521 cacgccaccg ttctccaggg tacctacaat cgatccaccc tcaaagctat cattgtacgc
    11581 ctgctcaata gcagcctggg tggtaatgtc catgttcttc aagacc
//
LOCUS       DBNGSX010000098        15530 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1392392, whole genome shotgun sequence.
ACCESSION   DBNGSX010000098 DBNGSX010000000
VERSION     DBNGSX010000098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 15530)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15530)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15530
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1392392"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="AC16CB_03300"
     CDS             complement(<1..469)
                     /locus_tag="AC16CB_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013158724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR3341526.1"
                     /translation="MKEAVIVAAVRTPIGAHGGALSAVRPDDMAALVIAEVVKRSGID
                     PAIVEEVYFGCANQAGEDNRNVARMATLLAGLPISVAAVTFNRLCASSLAAINAAARA
                     IKVGEGDVFIAGGVESMSRSPWVLPKSESAFPWGNLTAWDTTIGWRFPNQTLKE"
     gene            complement(466..1248)
                     /gene="fabG"
                     /locus_tag="AC16CB_03305"
     CDS             complement(466..1248)
                     /gene="fabG"
                     /locus_tag="AC16CB_03305"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HIR3341527.1"
                     /translation="MTSEQSGLSPAPRLDLADKVAIVTGGSRGIGQAIAWDLACNGAH
                     VALNYRRSADEAQQLIQQIQGLGRKGLAIQADVASFADAQRMVETVVRELGSLDILVN
                     NAGVNRDSVIWKMSEEQWDEVISIDLKGTFNYIRAAAPIFKEQGSGKIVNVTSINGLR
                     GKFGQSNYTAAKGGVNALTKTVARELGRYNVNVNAVAPGLIETDMVKQAPQQVRDLAL
                     SEIVLGRLGTPEEVAWIVTFLCSERARHITGEVITVDGGQYL"
     gene            complement(1270..2037)
                     /gene="fabG"
                     /locus_tag="AC16CB_03310"
     CDS             complement(1270..2037)
                     /gene="fabG"
                     /locus_tag="AC16CB_03310"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HIR3341528.1"
                     /translation="MKLAEKVALITGGANGIGRATALRFAREGARVVVSDVDAEGLAT
                     VCQEVEQAGGQAISAVGSVTDRSQVQDIVDKALNTFGRLDILINNAGINRDALTTRIK
                     DDEVRFMSDENWDLVVNVNMKGTWLCSQIASVPMMKQKFGRIVNTSSIGALGNIGQAN
                     YAASKAGVIGLTKTLALEWARFNIAVNCVAPGATKTRMTAGIPEKLLEGLVAKIPFGR
                     LAEPEEIASAHLFFASNDADYITGQVLFVDGGISLGV"
     gene            complement(2108..2566)
                     /locus_tag="AC16CB_03315"
     CDS             complement(2108..2566)
                     /locus_tag="AC16CB_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HIR3341529.1"
                     /translation="MGSNRGLTFDEFEIGDRYQSQARTVTEADVVAFAGISGDFNPLH
                     TDAEFGKSTPFGERIAHGMLLVAMATGMANWTGVFEGTTIALMEQLIRYKGVVKFGDT
                     IHLELEVMEKTPTSKPDRGVVKYAARVINQRGEVVIDGVWTLLMKRQINQ"
     gene            complement(2584..3357)
                     /locus_tag="AC16CB_03320"
     CDS             complement(2584..3357)
                     /locus_tag="AC16CB_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR3341530.1"
                     /translation="MSSEGNEFITLEIHDGVATLSLNRPPLNVMNLAMLQQMEATLEK
                     AASDTSIRVLVVQAKGKAFSAGVDVADHTADKVGEMIPLVDRVCKALADFPVPTLAAV
                     HGHALGGGCELVLCCDLAVMAAEASIGQPEIYLAALAPIAALRLPYLVGYRMAADILL
                     TGRRLTSEEAVKAGLVNAVVPQDQVKPWAIEKAGHLAGLSRAACILVKKAIRMGYGNW
                     AAAMPEMEKLYLQELMATDDAHEGLAAFMEKRSPEWRHR"
     gene            complement(3398..4444)
                     /locus_tag="AC16CB_03325"
     CDS             complement(3398..4444)
                     /locus_tag="AC16CB_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR3341531.1"
                     /translation="MDVLTDKKTTFEEIIYEKENWVARITINRPYNYNAYSTNCLEEL
                     AIAFRQASFDDEVAVIVYTGAGDKAFCTGGDVKEYQDRYTRSPHDYWKYMRLFGAYIE
                     SIVNTGKPVIARLNGMAVGGGNESQMACDLAIIAEHGWIGQVGSGVGSVAAGGATQWL
                     PIMVGDRRARYMLMTNRRIPPYQALEWGLVNEVVPSVKADGEFINHATPEQISMASKG
                     KDGYSIDLSLLDEAVNKLAQELVDKFPECIRYTKEQTNFWKNLAWHQTIGHARDWLSI
                     HYTSWEPLEGMTAFVEKRPPQYRLLRERAAQGKSSEFIWGPYSQTCSNCGAKALPEEF
                     TFCGECGNPLDGAS"
     gene            complement(4503..5429)
                     /locus_tag="AC16CB_03330"
     CDS             complement(4503..5429)
                     /locus_tag="AC16CB_03330"
                     /inference="COORDINATES: protein motif:HMM:NF019829.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator"
                     /protein_id="HIR3341532.1"
                     /translation="MNFSERPAVRTQFLVFTLFGEYVLPRGGSIWTSNLLSLMEVLGV
                     SERAARSTLSRMSRKGWITARKEGRRSRYSLTPRGWMLLKQGEKRIFEPTPQEWDGLW
                     YLVVYSLPEKKRRLRHSLRQGLSWLGFGPLSPGTWVSPHNRFIEVKNMCKDLGVQQHV
                     EIFSGAHVGLSTDQEIVRTCWDLAELEAQYKEFIRLYQGQYEGYLQQGDNEKAVSLEQ
                     CFVLRFWLTHQFQSFPRTDPNLHPALLPPDWIGFKARELFENYRALLESHANQYVDDV
                     LGAGNTNKESVQSIPLENRQTALDIPAKEVSV"
     gene            complement(5476..5841)
                     /locus_tag="AC16CB_03335"
     CDS             complement(5476..5841)
                     /locus_tag="AC16CB_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341533.1"
                     /translation="MPDIQQGNLQDEVDISKTSPAPKAQPQLDETANCYPQTPVSKHD
                     LSLPDLDFEPDPRLVAEGWERRFMADADRAKEAVELYQSLGFEVLTEEVKPTELSDLC
                     LDCQLVVCRYYVTVYTRKK"
     gene            complement(5846..6691)
                     /locus_tag="AC16CB_03340"
     CDS             complement(5846..6691)
                     /locus_tag="AC16CB_03340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HIR3341534.1"
                     /translation="MSYVTGIDVGSTYTKAVILDENNEMVGRGMTNTSFKLEEAARRA
                     YQLALEQAGLQEADIAYIVATGFGRHQIPFRDVNVTDLTASSFGASFLYPKTSTILDV
                     GGQTMKAIRLDGNQRVKSFRLNDKCAAGTGAFLEKTARYLGYSTEEIGTLAATSKTAV
                     PISGVCAVFAESEVINHLSQGAAPADIMYGSIISLVDRSVQLMKRVRMEPEFTLIGGI
                     LRFSSMAKVVREQLNTEVNVPEGDLVQFTTALGAAILGQRRLKKLQEAKADQETIEDK
                     SLAET"
     gene            complement(6705..7508)
                     /locus_tag="AC16CB_03345"
     CDS             complement(6705..7508)
                     /locus_tag="AC16CB_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013986.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HIR3341535.1"
                     /translation="MAYAAGVDVGSTQTKAIIVNENEEIVSRALVDTGANVVQAAENA
                     FREVLISGDVREEEVEFVVGTGYGRYRVTFGNTQVTEISCHARGAIKMFPNTHTVIDM
                     GGQDTKAIRVSPTGEVLDFCMNDKCAAGTGRFLGAASMALEIPLDNLGPTALLAEKPV
                     RISTTCTVFAESEVLSWLGKGKKIEDILLGVHQSIASRSVGLLRRVGIEEEITFTGGV
                     AKNVGMIKALEDNLEMKLNVSPDSHYMGALGAALFALDHIISKRAPLTA"
     gene            complement(7525..8823)
                     /locus_tag="AC16CB_03350"
     CDS             complement(7525..8823)
                     /locus_tag="AC16CB_03350"
                     /inference="COORDINATES: protein motif:HMM:NF017828.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HIR3341536.1"
                     /translation="MSDKQKPDGIVGRGNRDGAQLFRDWFNVLDETAARNGPSAYVFV
                     MGSMAEILNVFDLPLVFPEINSLQTAVRHLAHEFLSEAEDYGYSPDICGYVKADVALQ
                     LRGGDHPMGRVPKPALAVLTNACNTYIKWGEIWERMYNVPVFVMDVPGSREAGKQSWP
                     GEASFERDRRYIEEQIKELIHLCENITGRKFDIDRLRQALDHANRMNLAWKGVLELNK
                     SHPSVFNALTDGTIYLGVSNGLRGSVAGSQYFEDLLEEMQYKAANGIGTLTDEKYRLV
                     FVGVPCYPIFRRFNELFTEWGGTFVNSTYLWFASGGANRGFEYDLNRPLESLAEGTLL
                     SVRDAMDSMFHQNLALEEMIDEYSIDGVIFHPIKSCRTVSTGLADGRRALMAARDIPS
                     LFIESDMMDRRVVSEAQMKNRIDAFFEGLASRRQRETVTV"
     gene            complement(8839..10005)
                     /locus_tag="AC16CB_03355"
     CDS             complement(8839..10005)
                     /locus_tag="AC16CB_03355"
                     /inference="COORDINATES: protein motif:HMM:NF017828.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HIR3341537.1"
                     /translation="MTAEVFNTWRDQPVEEILYQCRELLEDMDYPTVQRWRENGGKVI
                     GHFQVYFPEEIAHAAGALPFKLRGAPVEPRHADSRFGSYLCSIIKSSLELALSKNIEL
                     DMFVTHPICDAARNLAAIWGRNFEYPCQILYLPQNANSSYTIQYLRDEYHRLQGEIEG
                     VTGRKISPEDLRRSLAVFNRNRTLLRQLYQIKRDTPWLCSADEAYALLAVGGLLPREE
                     HNELLETVLPLLKNRPNKRQDKIRVVFEGGFCEQPPFDMIRAIGQSCYVVDDDLLIGL
                     RWITDDVPVEGDPLYNLAQAYVEQSSYSPVQHDHRKPKEKMLLERIKNARAQAAIVTS
                     AKMCEPGLEEQVTYTRFLDEEGIPYFVSEFEENMTSFDHMEIQLETFVENLLFT"
     gene            10314..11225
                     /locus_tag="AC16CB_03360"
     CDS             10314..11225
                     /locus_tag="AC16CB_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HIR3341538.1"
                     /translation="MTASKYMTLEEAISTFVLDGSSLALGLAQETLIPFAAGWEIMRQ
                     SRRDLTLIGPISDILFDQMIGAGCVRWVRSAWVGNVITGSGYNFRRAVESGTLEIEDY
                     SNLSLAMALQAGALGVPFLPTYTALGSDLFQTNPSLNKVVCPFTGQVLTAVAALRPDV
                     AIVHVQRSDEQGNAHAWGNLGMTREACLAAEQVILTCEEIVPAEVISSDPNRVVVPGF
                     QVVAVVQAPWGAYPSPVPGYYNRDHQAFLDYQEASHTAERFASWLESRVVAPKDYDSY
                     LELIGKTRLAELVPKVSAPSAVVDFGY"
     gene            11277..12053
                     /locus_tag="AC16CB_03365"
     CDS             11277..12053
                     /locus_tag="AC16CB_03365"
                     /EC_number="2.8.3.-"
                     /EC_number="4.1.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase subunit beta"
                     /protein_id="HIR3341539.1"
                     /translation="MPVHSERYTAAELMVTAAANEIVNGEVVFVGMRLPLLGFQLAKS
                     THAPHAIGIFELGIVRDKPAPEPILTMGDLPNLYHAQWLTDTMGLMGMLQRGMVDVSF
                     IGGAQVDRFGNLNTSYIGPVRQPQTRLPGSGGACDLASLAKRHIIVMPHQKRRFVHNV
                     DYITSPGFGKGRGWREAQGLPRGGPAAVITTLGVLHFDQSTFEMVLTSIHPGVTVEEV
                     QANTGWALRVAPDITQTPEPSDEQLAILRKNDLEGYWTGV"
     gene            complement(12070..13623)
                     /locus_tag="AC16CB_03370"
     CDS             complement(12070..13623)
                     /locus_tag="AC16CB_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR3341540.1"
                     /translation="MSEIDPKIQELRKLKSQSRLGGGAERIESQHQRGRLTARERIDL
                     LMDKGSFREIDAFAEHRTTDFGLEKQKYLGDSVVTGWGTIGGRLVYVFSQDFTVFGGS
                     LGEVHAEKICKIMDMAMKNGAPLIGLNDSGGARIQEGVVSLGGYADIFLRNTLASGVI
                     PQISVIMGPCAGGAVYSPALTDFIIMVRNSSYMFVTGPEVVKTVTHEEVSFEELGGAS
                     VHSETSGVCHMAADSEADALYLIQKMLSYLPQNNMEDPPFVPNGDDPLRMEEALDKLI
                     PDDPGKPYDIKNAIRLIVDDRQFFEIHEAYAQNIVVGFARLGGHSIGIIANQPAVLAG
                     VLDINASEKAARFVRFCDAFNLPILTFVDVPGFLPGTAQEHGGIIRAGSKLLYAYCEA
                     TVPKLTVVTRKAYGGAYDVMSSKHIRGDVNLAWPTAEIAVMGPDGAVNIIFRRELAQA
                     KDPAKRKAELVDEYRKKFANPYVAASRGYIDDVIEPRETRPRLINALEMLSNKRDTNP
                     PKKHGCIPL"
     gene            complement(13722..14177)
                     /locus_tag="AC16CB_03375"
     CDS             complement(13722..14177)
                     /locus_tag="AC16CB_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341541.1"
                     /translation="MSNKNLQPSNASFGAQFSSTYPRPFSVTLLVFGVLTIAGINLYR
                     CIEAIVQWRFLAGLLPALVSYIMLSGLFWSLIGIPLAWSLWRGNRRAPQMTLWIVLVY
                     ITYYWLDRLLIRQGSASDNLPFTLGFAVLVLAFIFWTLSRPVAKAFFDT"
     gene            complement(14247..15281)
                     /locus_tag="AC16CB_03380"
     CDS             complement(14247..15281)
                     /locus_tag="AC16CB_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007976917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR3341542.1"
                     /translation="MKAVLFNQHGGPEVLEYADFEPPEPGEGQVLVWLRAAALNRMDV
                     WVREGWPGIKLEYPHISGADGAGEIVALGDGVTGWSLGDQVVINPSLNCGHCEFCLAG
                     QDNLCNKWALLGETVRGTLAEYVVVPSTNLFSLPADYDPHQAAAAALVFQTAWHSLIT
                     RGGVRPGETVLVVGASGGVNTAAIQIAKLAGAVVYVVGSSEDKLALAESLGADYLIDH
                     TKEENWSKAIFQLTNRRGVDVVVDNVGTTFPQSFRTARKGGRILTVGNTGGPKFEIDN
                     RYIFGKHLSIIGSTMSTHQDFARVMELIFAGKLKPVVDRTFPLSETRAAEEHLEKGNQ
                     KGKVTIDIRG"
BASE COUNT         3434 a         4287 c         3999 g         3810 t
ORIGIN      
        1 tctctttcag ggtttgattg gggaaacgcc agccaatggt ggtatcccag gcggtcaggt
       61 tgccccaggg aaaagcgctc tcagacttgg ggagtaccca cggagagcgc gacattgatt
      121 ccaccccacc ggcgatgaaa acatctccct caccgacctt gatagcccgc gcagcggcgt
      181 tgatcgcagc caggcttgac gcgcagaggc gattgaaggt gacggctgcc accgaaatgg
      241 gcagccctgc caggagggtt gccatgcggg caacgttgcg gttgtcctcg cccgcctggt
      301 tcgcgcagcc gaaatagact tcctcgacta ttgcgggatc gatccccgat cgttttacga
      361 cctcggcaat gacaagagct gccatgtcgt cggggcgtac agcgcttaag gcgccgccat
      421 gtgcgccgat gggtgtgcgc acagcagcga caattactgc ttctttcata ggtattggcc
      481 tccatccaca gttatgacct ccccggtaat atggcgggcg cgctcgctgc acaggaaggt
      541 cactatccag gctacctctt caggcgtgcc caggcgacct aatacgatct ctgacagcgc
      601 taaatcgcgc acttgctgcg gggcctgttt gaccatgtcg gtttcgatca atcctggcgc
      661 gactgcgttg acattcacgt tatagcgacc cagctcgcga gccacagttt ttgtcagcgc
      721 gttcactcct ccttttgctg cggtgtagtt cgattgccca aatttgccgc gcaacccgtt
      781 gatcgatgtg acgttgacga tttttcccga gccttgctcc ttgaaaatcg gcgcagctgc
      841 ccggatgtag ttgaaggtgc ctttcaggtc aatcgaaatc acctcgtccc actgctcctc
      901 gctcatcttc cagatgacac tgtcacggtt tacgccggcg ttgttcacca ggatatccag
      961 gctaccgagt tctcgcacca ccgtctcgac catgcgttgg gcatctgcaa aagaagccac
     1021 atcagcctga atcgcaagac cttttcgtcc gagaccctgg atttgctgga tcagttgctg
     1081 cgcttcatcg gcgctccgcc ggtagttgag ggctacatgc gccccattgc aagccagatc
     1141 ccaggcaatg gcttgcccaa tgccacggct tccaccggtg acaatggcga ctttatcggc
     1201 taaatccagc ctgggagctg gagacaatcc ggattgttct gatgtcatat tattcttttc
     1261 cttccagcgt tacacgccca gactaattcc gccatctaca aacaggacct gaccagtgat
     1321 gtagtcggca tcgtttgaag cgaagaacag gtgagcggag gcgatctctt cgggttcagc
     1381 cagcctgccg aaggggatct tggctaccag cccctccaag agcttctcgg ggatacccgc
     1441 ggtcatcctg gtcttggtcg cacctggcgc aacacagttc accgcgatgt tgaaacgcgc
     1501 ccactccagc gcaagtgttt tggttagacc gatcacgccc gccttggagg cggcgtaatt
     1561 ggcctgtccg atgttaccca gcgcgccgat tgaagacgta ttcacgatgc gaccgaattt
     1621 ctgcttcatc atcggcactg aggcgatctg cgagcacagc caggtgcctt tcatattgac
     1681 attcaccacc aggtcccagt tctcatccga catgaagcgc acttcatcat ccttgatgcg
     1741 ggtggtgagc gcatcgcgat tgataccggc attattaatc aggatgtcta agcgcccaaa
     1801 tgtatttaag gctttatcaa caatgtcctg aacctgggat cggtcggtga cactgcctac
     1861 ggcagatatg gcttgtccgc cggcttgttc gacctcctgg cagacagttg ccagaccttc
     1921 agcgtctaca tcgctgacca ccacccttgc cccttcacgg gcgaagcgca aggcagttgc
     1981 ccggccaatc ccattggcgc cccccgtaat taaagcgact ttttccgcaa gcttcatttt
     2041 tccctccgca ttttcttaaa gaattgaaac gtcaattcac tgatttactg ctcgggcttc
     2101 aaagttttca ttgattgatt tgtcttttca ttagcagggt ccacacgcca tcgataacca
     2161 cttcacctct ctggttgatc acccgcgcag cgtacttgac aacccctcga tctggtttgg
     2221 atgtaggggt cttttccatc acttctagct caaggtggat cgtgtcgcca aatttcacca
     2281 cgcctttgta tctgatgagc tgttccatta gggcaatggt ggtgccttca aacacgcctg
     2341 tccagtttgc catcccggtc gccatcgcca ccagcagcat gccatgggcg atccgttccc
     2401 caaagggtgt gctcttaccg aactctgcat cggtatgaag ggggttgaaa tcccccgata
     2461 tacccgcgaa ggcgactaca tcggcttcgg tcactgtgcg agcttgagat tggtatcgat
     2521 caccgatttc aaattcgtca aaggtcagtc cgcgatttga gcccatttac cctcccataa
     2581 atctcatcga tgtctccact cgggagatcg tttttccatg aaagctgcca gcccttcatg
     2641 cgcatcgtcg gttgccatta attcctgcaa gtagagcttt tccatttcgg gcatggctgc
     2701 tgcccaattg ccgtatccca tgcggatagc ttttttaacc aggatgcaag ctgcccggct
     2761 cagaccagcc agatgcccag ctttttcaat agcccatggt ttcacctggt cttgtgggac
     2821 gactgcgttc acaagcccag ccttaacagc ttcctcggat gttagccggc gccccgtcaa
     2881 caggatatcg gcagccatgc gataaccaac caggtatggc aatcgcaacg ctgcaattgg
     2941 agccaatgct gccaggtaga tctcgggttg cccgatgctg gcttcggctg ccatgaccgc
     3001 caagtcacag cacaacacca attcacagcc accgcccagg gcgtgtccgt gtaccgcagc
     3061 cagtgtggga acgggaaaat ctgccagcgc tttgcatacc cggtctacca ggggaatcat
     3121 ctcacctact ttgtcggcgg tgtggtctgc tacatcaacg cccgctgaaa aggccttgcc
     3181 ctttgcctgc actaccagca cgcgtatgga ggtatcggaa gctgcctttt ccagggtggc
     3241 ttccatttgc tgtaacatcg caaggttcat cacatttagg ggaggccggt taaggctcag
     3301 tgtggcgacg ccgtcgtgta tttcgagtgt gatgaattca tttccttcgg aagacatgtt
     3361 gctatccttg gttcgttact atgatctgac tgcttgccta acttgcccca tccagagggt
     3421 taccgcactc gccgcagaag gtaaattcct ccggcaaggc tttagcccca cagttggagc
     3481 acgtttggct atatggaccc caaataaatt cgctgctttt accttgggca gcgcgctcgc
     3541 gcaatagacg atactgaggg ggacgcttct caacgaaggc agtcatgccc tccagaggct
     3601 cccaggacgt gtagtggatc gatagccaat cgcgggcgtg cccgatggtc tggtgccagg
     3661 ccaggttttt ccagaagttg gtttgttcct tcgtgtatcg aatgcattct gggaatttgt
     3721 caaccagctc ctgcgccagt ttgttcaccg cctcatcgag caaagataaa tcgatgctgt
     3781 agccgtcctt gcctttagat gccatcgaga tttgctcggg cgtggcgtgg ttaatgaatt
     3841 cgccgtctgc tttcaccgag ggcactactt catttaccaa accccattcc aacgcctggt
     3901 acgggggtat gcggcggttg gtcatcagca tgtaacgggc gcgccggtct cctaccataa
     3961 tcggtaacca ttgggttgct cccccggcag ccacactgcc taccccgctg cccacctgcc
     4021 cgatccagcc gtgctcagcg atgatcgcca gatcgcaagc catctgcgac tcgttgccac
     4081 cccccacggc catgccattc agacgggcga tgacgggttt cccggtgttt acaatgcttt
     4141 cgatgtaagc gccgaatagg cgcatatatt tccaatagtc gtgcggcgat cgggtataac
     4201 ggtcctgata ttctttcacg tctccgccgg tgcaaaatgc cttgtcacct gctccggtgt
     4261 atacaatcac agccacttcg tcatcaaagc tggcctgccg gaatgcgatt gctaattctt
     4321 ccaggcagtt ggtgctgtag gcattgtagt tataggggcg gttaatcgtg atacgggcta
     4381 cccaattttc tttttcgtaa atgatctcct cgaatgttgt ttttttatcc gtaagcacat
     4441 ccattgtggc ctccaagctc agcactcatt gaacttctgc tatttcctgt gtcccttccc
     4501 atttacacgg acacttcttt cgccggaatg tctagcgcag tctggcgatt ttccaggggg
     4561 attgattgga cgctctcttt gttggtgttc cccgcgccca gtacatcatc aacgtattgg
     4621 ttagcgtgtg attcgagcaa cgctcgataa ttctcgaaca actcgcgcgc cttgaatcca
     4681 atccagtcgg ggggcagcag cgctgggtgt aggttcgggt cggtgcgcgg gaaggattga
     4741 aattggtgag tcagccagaa ccgcagtaca aagcattgtt ccagggagac ggctttctca
     4801 ttgtctccct gttgcaggta accctcgtat tgaccctggt ataacctaat aaattcctta
     4861 tattgcgctt ccaattcagc caagtcccag catgtgcgca cgatttcctg gtctgtagac
     4921 aggccgacgt gtgcgcctga aaaaatttcc acgtgctgct gaacccccag gtctttgcac
     4981 atattcttta cttcgatgaa acgattgtga ggagataccc aggttcccgg tgatagtggc
     5041 ccgaagccca accagctcaa gccttggcgc aaagagtggc gcagccggcg ttttttctca
     5101 ggcagggaat acaccaccag gtaccataga ccatcccatt cctggggagt gggttcgaaa
     5161 atgcgttttt caccttgttt caacagcatc cagccgcgtg gagttagtga atatcggctg
     5221 cgtcgcccct cttttctggc agtgatccag cccttgcggg acatgcgtga aagagtggaa
     5281 cgggcagcac gctcgctgac ccccagaacc tccatcaggc taagcagatt gctggtccat
     5341 atagaacccc cgcgcggtaa gacatactca ccaaataagg taaaaacgag gaattgggtt
     5401 cttacagccg ggcgttcaga gaagttcatc aacctacata tcctcggtca gcatccttat
     5461 ttaagagaat aggggtcatt tcttgcgagt gtacacggta acgtagtaac ggcagacaac
     5521 cagttgacag tctaagcaca aatcgctcaa ttccgttggt tttacctctt ccgtcaagac
     5581 ctcaaaacca agcgactggt atagctcaac agcctctttc gctctatccg catcggccat
     5641 aaaacgccgc tcccaccctt ctgctaccag cctcgggtct ggttcaaaat cgagatccgg
     5701 caaagaaaga tcatgtttgc ttactggcgt ttgggggtaa cagttggctg tttcatctag
     5761 ttggggctgg gccttgggag ccgggctggt tttcgagata tcaacctcgt cctggagatt
     5821 accctgttgg atatcaggca tatcgttatg tctcagctag cgatttatct tcgatcgtct
     5881 cttggtcagc ctttgcttct tgcagtttct tcaaccggcg ttggccgaga atcgcagcac
     5941 ccagcgccgt agtaaattgg accaggtcac cctcaggcac gtttacttca gtgttcaact
     6001 gctcccgcac gactttagcc atggagctga agcgcagtat tccgccaatc agggtgaact
     6061 cgggttccat gcggacacgc ttcatcaact gtacagaccg gtcgaccaac gaaatgatag
     6121 atccatacat gatgtcggcc ggcgccgcgc cttgagagag atggttgatg acctctgatt
     6181 cggcgaacac tgcgcaaacc ccagagatgg ggacagccgt ttttgaagtc gctgccagtg
     6241 tgccaatctc ctcggtagag taacctagat agcgggctgt tttttccaga aaggcacccg
     6301 taccagctgc gcatttgtca ttcaggcgga aggacttcac gcgctgattc ccgtccagcc
     6361 ggatggcttt catggtctgc ccacctacat ccaggatggt ggacgtcttg ggatagagga
     6421 aggatgcccc gaaggagctg gcggtgaggt cggttacgtt cacgtcgcga aaaggaattt
     6481 ggtgacgacc aaaacccgtg gcaacaatat aggcaatatc ggcttcctgc aggcctgctt
     6541 gctccaatgc gagctgataa gcgcgccgcg ccgcctcctc cagcttaaag ctggtgttgg
     6601 tcatgccccg accgaccatc tcattgtttt catccaggat gacggctttg gtatacgtgg
     6661 atccaacgtc gataccggta acgtagctca tggttgctcc tgccttatgc ggtgagaggc
     6721 gctcgttttg aaatgatatg atctagtgca aagagggccg cgcccaaagc acccatataa
     6781 tgcgaatccg ggctgacatt caatttcatc tccaggttat cctccagggc tttgatcatg
     6841 cctacgttct tcgccacccc gccagtgaag gtgatctctt cttcaatccc gacccggcgc
     6901 agcaatccga ccgagcgcga ggcaatcgac tgatgcactc ccaacaggat atcttcgatt
     6961 tttttgcctt tgcctaacca ggaaagcact tccgattcag caaaaacggt gcaggttgtg
     7021 ctgatccgga ccggtttttc agctagcaag gcagtgggtc ctaggttgtc cagggggatc
     7081 tccagtgcca tggatgccgc cccaaggaac cgtccggtgc cagccgcgca tttgtcgttc
     7141 atgcagaagt ccaagacttc gccggtcggg ctgacgcgaa tagcctttgt atcttgccca
     7201 cccatatcga tcacggtgtg ggtgttgggg aacattttta ttgccccgcg tgcgtggcag
     7261 ctgatctctg tcacctgcgt attgccaaag gttactcggt agcgtccgta acctgtacca
     7321 acaacaaact ctacctcctc ttcacgcaca tcaccggaga taagcacttc ccggaaggcg
     7381 ttttcagcag cctgaacgac attggcgccc gtatctacga gggcgcgact gacgatctct
     7441 tcgttttcat taactatgat cgccttggtc tgtgtggaac caacatctac acctgcagca
     7501 tatgccatgg aaccctctcc tgactcaaac agtcaccgtt tctctttgtc ggcgagatgc
     7561 caaaccttca aaaaaggcat caatgcggtt cttcatttgg gcttcagata ccacccggcg
     7621 gtccatcata tccgactcga tgaacaggct ggggatatct cgggcagcca tcaacgcccg
     7681 ccgcccatca gctaggccag tcgatacagt gcggcagctc ttgatgggat ggaaaatgac
     7741 gccatcgatg ctatactcgt caatcatctc ctccaaggct aaattttggt ggaacatgct
     7801 gtccattgca tctcgcacac tgagcagtgt gccttctgcc aggctctcca gcgggcgatt
     7861 caggtcgtac tcaaaccccc ggttggcgcc gccagatgca aaccacaagt aagtggaatt
     7921 tacaaaggtg cctccccact cggtgaatag ttcgttgaag cggcggaaaa tggggtagca
     7981 tggcacaccg acgaaaacca ggcgatattt ttcgtctgta agcgtgccaa ttccgttggc
     8041 agccttgtat tgcatttctt ccaacaggtc ctcgaaatac tggctgccag ccacactccc
     8101 tcgtaagccg ttcgagacgc ccagatagat ggtcccatcc gtcaacgcat tgaacaccga
     8161 aggatggctt ttattgagct ccaggacacc cttccaggcc aggttcatgc ggttggcgtg
     8221 gtccagagcc tgtctcaggc ggtcgatatc aaacttgcga ccagttatgt tctcacataa
     8281 gtggattaat tccttgattt gttcttcaat atagcgccgg tcccgttcaa aggaagcctc
     8341 acctggccag gactgctttc cggcttcacg cgatccgggt acatccataa caaaaactgg
     8401 gacgttatac atgcgctccc agatttctcc ccatttgata taggtgttgc aggcgtttgt
     8461 cagcacagcc agggcgggct ttggcacacg ccccatgggg tggtcaccac cgcgcaattg
     8521 cagggcgaca tcggctttta cgtagccgca aatatccggc gagtaaccat aatcttcggc
     8581 ttcactcagg aactcgtgag ctaggtggcg caccgcagtc tgcagtgagt tgatttctgg
     8641 aaacaccagg ggcagatcaa aaacgttgag tatttcagcc atgctgccca tcacgaacac
     8701 gtaagcagac ggcccgttac gtgctgcggt ttcatccaac acattaaacc aatcgcggaa
     8761 cagctgggcg ccgtctcgat tgcctcgtcc cacaatacca tctggttttt gtttatcact
     8821 catatttctc acctttggct atgtaaataa gaggttctca acgaatgtct ccagctggat
     8881 ttccatgtgg tcaaagctgg tcatgttttc ttcaaattca ctgacgaaat agggtatacc
     8941 ttcttcatcc aagaagcggg tgtatgtcac ctgttcttcc aggccgggtt cgcacatttt
     9001 tgccgaagtg acgatggctg cctgagcgcg agcgttctta atgcgctcaa gcaacatttt
     9061 ctctttcggt ttgcggtggt cgtgctgaac ggggctgtaa gaggattgct ccacgtacgc
     9121 ttgcgccagg ttgtagagcg ggtcaccctc cacaggcacg tcatcggtga tccagcgcag
     9181 gccgatcaac aggtcgtcat ccacgacgta gcatgattgc ccgatagcgc ggatcatgtc
     9241 aaagggaggc tgctcgcaga agccgccctc gaacaccacg cgaattttgt cttgtctttt
     9301 attgggcctg ttcttcaata agggcagcac agtctctagt agttcgttgt gctcctcgcg
     9361 tggaagcaaa ccgccgacag ccaataatgc gtaggcttcg tctgcggagc ataaccaggg
     9421 ggtgtctctc ttgatctggt aaagctggcg gagcaaggtg cggttgcgat taaatacagc
     9481 cagtgaacgg cgcaagtcct ctggactgat tttacgccca gttacgcctt cgatctcacc
     9541 ctgcaagcga tgatattcat ctctcaggta ttggatagtg tatgatgagt tggcgttctg
     9601 cggcaggtag agaatctggc acggatactc aaaattacgc ccccaaatgg cagccagatt
     9661 gcgagcggca tcgcaaatgg ggtgggtgac aaacatgtct aattcgatgt ttttactgag
     9721 tgccagttcc agggaggatt taataatcga acataagtag gagccaaagc gggaatcggc
     9781 atggcgtggt tccactggtg cgccacgtaa tttgaaaggc agcgctccag cggcatgggc
     9841 gatctcttcc ggaaaataaa cctggaagtg accgatcacc ttccccccgt tttctcgcca
     9901 gcgctgaacg gtgggataat ccatgtcttc tagcagttcc cggcactggt acaggatctc
     9961 ttctactggt tgatcgcgcc atgtgttgaa aacctcagcc gtcatgtgat cactccggga
    10021 taatatgctt cgattttata aggtatcgtc actgaaatgc gacatatttc agtatacctc
    10081 aatttcgtta attgtcaata aattgggcga gttaatttgt ttatcgaatt ctggtcatcc
    10141 ttgcgaggat attggctata atttggccta tcagcatacc tacaacctgt ggtatttctt
    10201 atatgcacac acaagctcaa gcagggtaga tagagggaga agcctagctt ctgcattttt
    10261 tcctgcagcg gagaaagtcc caagtgcaat taatctctgg acgaggaggg tctatgactg
    10321 cgtcgaaata catgactctg gaagaagcga tcagcacctt tgtattggat ggttcatcgt
    10381 tggcgctggg tctcgcccag gagacgctta tcccctttgc tgccggttgg gaaatcatgc
    10441 gccaatcccg gagggatctc acattaatcg gcccgatttc ggatattctt tttgaccaga
    10501 tgatcggtgc tggttgtgtt cgctgggttc gttctgcctg ggtgggaaat gtcatcacgg
    10561 gctcaggata caatttccga cgagcagttg agagcggtac cttggagatt gaggattatt
    10621 ccaacctgtc attggctatg gccttgcaag ccggtgcgct gggtgtgcct ttcctcccta
    10681 cctacactgc actgggtagt gacttattcc aaaccaatcc cagcctgaac aaggtcgttt
    10741 gtccttttac cggtcaggtc ctgacggccg tggcagcact caggccggat gtggccatcg
    10801 tccatgtgca gagatctgac gagcaaggca acgcccatgc ctggggtaac ctgggcatga
    10861 ctcgcgaagc ctgtttagct gccgagcaag tcatcttgac ttgcgaagag atcgtacccg
    10921 ccgaagtgat cagtagcgat ccaaaccgcg tagtagttcc tggcttccag gttgtagcgg
    10981 tggtccaggc gccctggggc gcttatccat cgccagttcc cggttactat aaccgagatc
    11041 accaggcttt cctcgattac caggaagcaa gccacaccgc agagcgtttt gccagctggc
    11101 tggagagtcg ggtggtcgcg ccaaaggatt atgatagcta tctggagctg attgggaaaa
    11161 cacgcttggc tgagcttgtg cccaaagtaa gtgcgccctc ggcagtggtg gactttggat
    11221 attgattgcc cgaagacagg cattgtcaga ccagatcaga gcaggcgagg atgatcatgc
    11281 cagtacattc tgagcgttac acagccgctg aattaatggt cacagcagct gcaaatgaaa
    11341 ttgtcaatgg ggaagtggtc tttgtcggaa tgcgcttacc gttattaggc ttccaattgg
    11401 cgaagtccac ccatgccccg catgccattg ggatttttga attaggcatt gtccgcgata
    11461 aaccggctcc ggagcctatt ctcacaatgg gggatttgcc taatctatac cacgcgcagt
    11521 ggctcacgga tactatggga ttaatgggga tgctccagcg agggatggtt gacgtgagct
    11581 ttatcggcgg cgctcaggtg gatcgttttg gcaatttgaa taccagctac attggaccag
    11641 tgcgccaacc acagacccgc ttgccgggta gcggtggggc ttgcgacctg gcttcactgg
    11701 ctaagcggca tattattgtg atgcctcacc aaaagcgtcg atttgttcat aacgtggatt
    11761 acatcacttc gcccggcttc ggaaagggtc ggggctggcg agaagcacaa ggcttgccgc
    11821 gcggcggtcc agcagcagtg atcacaactc tgggcgtgct gcatttcgac cagtctacct
    11881 ttgagatggt gctcacctca atccatccgg gggttacagt tgaggaagtg caagcaaaca
    11941 cggggtgggc actccgtgta gcgccggaca ttacacaaac gccagagcct agtgacgagc
    12001 agcttgcaat cttgcgcaaa aatgatttgg aaggttattg gacaggggtc tgaccaacag
    12061 ctagagattc tagagcggaa tgcagccgtg tttcttcggc ggattggtat cgcgcttgtt
    12121 tgagagcatc tccagggcat tgatcaggcg cgggcgcgtc tcgcgcggct cgatcacatc
    12181 gtcaatatag ccgcgtgaag ccgctacata cggattggcg aatttctttc ggtattcgtc
    12241 aactaactca gccttgcgtt tggcggggtc tttcgcttgt gccagttctc tgcggaaaat
    12301 aatgtttacc gcgccgtctg gccccatcac ggcgatctct gccgtaggcc aggccagatt
    12361 aacgtctcct cggatgtgct tgctgctcat cacgtcatac gcgccgccgt aagctttacg
    12421 ggtgaccacc gtcaactttg gcacggtggc ctcgcaatag gcatacagca acttggagcc
    12481 cgcgcgaata atgccgccgt gttcctgcgc agtgccaggc agaaaaccag gcacatccac
    12541 aaaggtcagt atagggagat tgaatgcatc gcagaaccgc acaaagcggg ctgctttctc
    12601 ggatgcattg atgtccaaaa cgccagctaa tacagcgggc tgattggcaa taataccaat
    12661 gctgtggcct cccaagcggg cgaagcccac gacgatgttt tgcgcgtatg cttcgtggat
    12721 ttcaaaaaat tgcctgtcgt ccacgatcag gcggatcgca ttttttatgt cgtagggttt
    12781 ccccgggtcg tcagggatca gtttatccag cgcctcttcc atgcgcagtg gatcatcccc
    12841 gttaggcacg aagggagggt cttccatgtt gttctggggc aggtaggaga gcatcttttg
    12901 gataagatag agggcatcag cttcactgtc ggcagccatg tggcagacgc cagacgtctc
    12961 agagtgcaca ctggcgccgc ccaactcttc gaaggaaacc tcctcgtgtg tcaccgtctt
    13021 aaccacctct ggtccggtga cgaacatata cgaggagttg cgcaccatga tgatgaagtc
    13081 agtcagggca ggagagtaga ctgctccccc ggcacaaggt cccataatca cactaatctg
    13141 ggggataacg ccagaagcca gggtgttcct caggaatata tctgcataac ctcccaagga
    13201 gactacccct tcttggatgc gtgccccgcc ggaatcgttt aacccgatta ggggcgcgcc
    13261 attcttcatt gccatatcca ttatcttgca gatcttctca gcatgcacct ctcccaggct
    13321 gccgccaaag actgtaaaat cttgcgagaa gacatagact aacctaccgc caatggtgcc
    13381 ccaaccggta actacactgt cgcccaggta cttctgtttt tccaggccaa aatctgtcgt
    13441 gcggtgttct gcaaatgcat caatctcgcg gaaagaaccc ttatccatca gcagatcaat
    13501 gcgctcgcgt gcggtcaggc ggccgcgctg gtgttgcgat tcaatgcgct cagcaccgcc
    13561 gcccagccgg ctctggcttt tcagtttgcg taattcttgg atcttaggat caatttcgct
    13621 cattcaacgc tccaaaagat aagcttcctt cttgatcgca tttcaatttt acccatgctg
    13681 tatacgggaa tatagaaaag ctgattatag gctatttttt gctatgtatc gaaaaatgct
    13741 ttggcaactg gtcgagataa cgtccaaaaa ataaatgcta gcaccagcac agcgaatccc
    13801 agtgtgaagg gcaggttatc ggatgctgat ccttgacgta taagcaaccg gtctaaccag
    13861 tagtaggtta tgtacaccag gacaatccat aaggtcattt gaggtgccct gcgattgccg
    13921 cgccataggc tccaggctaa agggatccca atcaggctcc aaaataatcc acttagcata
    13981 atataagata caagcgccgg gagcagcccg gcgagaaagc gccattgtac gatggcttca
    14041 atgcagcgat ataagttaat cccagcaatg gttaaaaccc caaataccag cagggttacg
    14101 ctgaaaggtc gaggataggt tgatgaaaat tgggcgccaa atgaagcgtt cgagggctgc
    14161 agattcttat tggacataga aagaattcta cccttaactg gtcacccttt tgtgatcaat
    14221 ccaaggtttg ggtttatctg cagcaattat cctcgaatat caatggtaac tttccccttt
    14281 tgattcccct tttctaggtg ttcctccgct gcacgcgtct ctgagagagg gaacgtacga
    14341 tccacaactg gtttgagctt gccagcaaag atcagttcca tcacgcgggc aaagtcctgg
    14401 tgggtgctca tcgtagaccc aataatggac aagtgtttgc caaatatata acggttatcg
    14461 atctcaaact ttgggccgcc agtattgcca acagtcagga tgcgcccacc cttgcgcgct
    14521 gtccgaaagc tctgcgggaa ggtggtgccc acattgtcca ccacgacatc cacaccgcgc
    14581 cggttggtca attggaatat ggccttggac caattctcct ccttggtgtg gtcaatcaga
    14641 taatctgccc ctagagactc tgcaagagcc agtttgtcct cgcttgaacc aaccacgtag
    14701 acaacggctc ccgccaattt ggcaatctga atagctgcgg tgttcacccc tcccgaagct
    14761 cctaccacca gaactgtctc acctgggcgc accccgccgc gcgtgatgag cgaatgccag
    14821 gcggtttgaa agaccagcgc cgctgcagca gcctggtgcg ggtcgtagtc agcgggaagc
    14881 gagaagaggt tagtgctcgg aacgactaca tattccgcca acgtgccgcg cactgtttca
    14941 ccgagtagag cccatttgtt gcacaggtta tcttgaccag ccaggcagaa ctcgcagtgc
    15001 ccgcagttca ggctgggatt gatcactacc tggtcgccaa gagaccatcc cgtgacgcca
    15061 tcccccaggg cgacgatttc cccagcccca tccgccccgc tgatatgagg gtattcgagt
    15121 ttgatgcctg gccagccttc ccgcacccaa acatccatgc ggttcaatgc agccgccctg
    15181 agccatacca gtacttgccc ctctcctggc tcggggggtt caaaatcagc atactccagg
    15241 acttcaggac ccccgtgttg attaaaaaga acagctttca agatatttct cctgaatgac
    15301 aaacaggttt tctgcaacca tacccaactc gcccatatag gataaagcta cctccataca
    15361 attggccact ctgccttact cgttgaaagc tggtacgaag acaatcacgc ttgggattaa
    15421 gggagcaggt gcaattcccg gatgtggatg ttggccctct ctccgctggc tacgtcgagg
    15481 atttcgatgc gaactttgga aactcgctcg ggagcatctg gcaatggcat
//
LOCUS       DBNGSX010000099        16219 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1323312, whole genome shotgun sequence.
ACCESSION   DBNGSX010000099 DBNGSX010000000
VERSION     DBNGSX010000099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 16219)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16219)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16219
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1323312"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="AC16CB_03385"
     CDS             <1..473
                     /locus_tag="AC16CB_03385"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR3341543.1"
                     /translation="TRALAAIKAGKQQYLYLGNLDAKRDWGYAPDYVAALWKMLQHDQ
                     AGDFVIGTGEAHSVREFLDEAFGYINMDWKGCVRIDPRYYRPTEVDYLLADPGKAQRE
                     LGWEPRVFFRDLVRIMVDADLELMGLKSPGEGKRILEQRHGKWHRWESQVVSME"
     gene            473..1498
                     /locus_tag="AC16CB_03390"
     CDS             473..1498
                     /locus_tag="AC16CB_03390"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015149721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR3341544.1"
                     /translation="MEENRLSSKLYRALITGGAGFIGSHLAEALLSRGHHVTVIDNLS
                     TGRFENIQHLIDHPRFRFAIDTITNEVVMDRLVSECDVIFHLAAAVGVMLIVEKPVHT
                     IETNVMGTEAVLKAALRYRAKVLIASTSEVYGKGNHIPFREDDDVVLGPTSRNRWAYA
                     ASKMVDEFLALAYHREKGLPVVVYRLFNTVGPRQTGRYGMVIPRFVQQALRGDTLEVY
                     GDGQQSRCFLHVTDAVDAILALADCSEAVGEVFNIGSVEEITIDALARRILDRAQQIT
                     KKSENVSRQIKYVPYDQAYAIGFEDMRQRVPEVTKIAQYTGWKPKRSLEQILLDILED
                     TVKKSTV"
     gene            1495..2622
                     /gene="wecB"
                     /locus_tag="AC16CB_03395"
     CDS             1495..2622
                     /gene="wecB"
                     /locus_tag="AC16CB_03395"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008735112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HIR3341545.1"
                     /translation="MTKPHRILSILGTRPEVIKLAPVILEMERKPGQFISRVCATAQH
                     RQMLDQTLDVFGLKVHHDLNIMTPGQTISQVTARAVEGLDHVISSERPDLVLVQGDTT
                     TAFCGALAAYYHQVRVGHVEAGLRTGDKYAPYPEEINRRLIGQLADFHFAPTERARQA
                     LLLEGFPEANIFVTGNTVVDALLWVRERVRKAVPELPDGINEWLQDRQMVLVTGHRRE
                     SFGEGFENICQAIRQVADILPEVVFIYPVHLNPNVREPVGRILGGHERIHLIDPLPYE
                     PFVWLMDRATVVLTDSGGIQEEAPSFGKPVLVMREVTERPEGIDAGVARLVGVRQEGI
                     AASLIELLTHPQARQAMAAVQNPYGDGLAAKRITNILERML"
     gene            complement(2653..4713)
                     /locus_tag="AC16CB_03400"
     CDS             complement(2653..4713)
                     /locus_tag="AC16CB_03400"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF015531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR3341546.1"
                     /translation="MMKNPFNSTFINLRFGLAFAVVFALFALGCQALTNTAIPPTPTP
                     IPEPTPIPTRLPPVPVSPGEENPDEPVFITGDIPYTSPFFINTIFEPFVLLEDQAGFA
                     SRDKEFIFRLEGQAIGPVEIHNDQRVSYSLALPSIPQGTQLDVDNDGKDEVGVQIFAV
                     AYWSNTWGGPFLERRDGKGWSTSYVSTIVDPDREDEIVGGILVVWAPDDQQSFPTGFG
                     EDGLLFTKDDPAAPIPSGYNLVDINQEPFSFYKEARPNITLSEGASAVNDFSTMSYAD
                     AFQAMIEKVERAYPFTREKNISWETLKDEYAARIANVSNDKDFYRVLHDFTQEIPDGH
                     VGMQINGEIFFEDNGGGFGLNLTELSDGRVLVTNVIPNSPGERAGIRIGAELLTWEGK
                     PISEAIDAVNPYFGPYSTQHNRRIGQVLFLTRVPPNTNVTVTFQNPGSNAEQEARMRA
                     DVDYDSLFAAIPELSKDELIIPVEGEILDESGLGYVRVNTFSADYSLIARLWEHFIKG
                     LIDNEIPGLIIDLRVNSGGSGHLATDFAGYFFEEEITLYESLYYSEESNRFEASGLTS
                     KIEPGPLFYAGQIAVLVGPDCVSACEGFAYALTQNGRSTVVGHYPSAGAYGEVGRGQY
                     TLPGDIKMQFPTGRYVMPDGQLALEGVGVIPDVVVPVTEESALGEEDAVLEAAVSTLL
                     ESIK"
     gene            complement(4717..5859)
                     /gene="chrA"
                     /locus_tag="AC16CB_03405"
     CDS             complement(4717..5859)
                     /gene="chrA"
                     /locus_tag="AC16CB_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008183941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HIR3341547.1"
                     /translation="MKPHQTSLLRLLELAGLFLRLGFTAFGGPAAHIAMYRDEVVTRR
                     KWLSDQDFLDLLGATNLIPGPNSTEMVIHIGLRRAGWIGFLLAGTCFILPAMSIVLIL
                     AWLYANYGTTPQSGWLMKGIQPVVIAIIIQALWGLGQKAIKGTQTAFAGLAALILYFL
                     NLHPILILLICGSLVMVFENAKRLRSYNMTCAILIGGSSLVPLGVAPFSLGLLFLTFL
                     KIGAVLYGSGYVLLAFLHADFVARLGWLTEGQLLDAIAIGQVTPGPVFTSATFIGYLL
                     GGIPGAILSTVAIFLPAFIFVAASSVWIPRLRNSPWLASLLDGVNVASLGLMAGVTWQ
                     LARSSLNNPAGILLLLISGFLLVKYKINTTWLIFAGALIGYLFRLG"
     gene            complement(5923..6477)
                     /locus_tag="AC16CB_03410"
     CDS             complement(5923..6477)
                     /locus_tag="AC16CB_03410"
                     /inference="COORDINATES: protein motif:HMM:NF019494.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HIR3341548.1"
                     /translation="MPIIKRYPNRKLYNTQAKQYITLQGIAELIFEGEEVQVIDHATR
                     EDLSAVTLTQILFELEKKSGFLPLKILANLIKVSNNRLDALQRYLASRIGPSNPVDEE
                     IKQRIQILVQRGELAETEGKRLQEKLIHLGIGRRSNQPTPFIWDMVQFLEKQNIPTRD
                     DLEPIMAQLDSLSFELENLYKAQK"
     gene            complement(6484..6855)
                     /locus_tag="AC16CB_03415"
     CDS             complement(6484..6855)
                     /locus_tag="AC16CB_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HIR3341549.1"
                     /translation="MPTKEKVEGSAKGEELKPLFEAARKVLLAGIGAIALAQDEIEEF
                     VNRLVERGEIAEQDGKKLIRELKDKRKKEAQQFQDEVTKRVEETLLQMNIPSKEDIKS
                     LSDKIATLSKKVDELKKAQQQ"
     gene            7017..7673
                     /locus_tag="AC16CB_03420"
     CDS             7017..7673
                     /locus_tag="AC16CB_03420"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3341550.1"
                     /translation="MKILSVSDVLVPFIYSSQVRAKFSGVDFVIAGGDLPYYYQEFIL
                     SMLDVPLFFVRGNHDKLEEHSQVGKRSGPDGGVDMHLRTEECKGILLAGVEGCIRYRL
                     GDFQYSQSEMWRHVWRLIPSLLKNRIQHGRYLDIFITHAPPRGIHDQDDLPHHGVDAF
                     RWLIQTFHPAYHFHGHIHVYRPDAPVESLVGMTKVINTYGFRETLITNQRQINTGLRA
                     "
     gene            complement(7640..9367)
                     /locus_tag="AC16CB_03425"
     CDS             complement(7640..9367)
                     /locus_tag="AC16CB_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3341551.1"
                     /translation="MNYDPNQRNLDTYRYITSLQDFRRARSQAKLKDLVARVTGESTE
                     LLSYEEVRQKLRAYASSNRGLQDIPLDAIVGSVGRYNDFTRDFLPRQDFTQDRWARVK
                     AASEASRGLPPIDVYKIGEAYFVQDGNHRVSIARQYGSKTIEANVTEVLVRVPLDPDT
                     QPDDLILKAEYAGFLERTHLDELRPEADLSVTIPGQYPVLEEHIAVHRHYMGQDQQRF
                     IPNDEAVADWYDSIYLPAIRAIQEGGILRNFPDRTETDLYLWIAEHRAALENEWGVEI
                     KTEVAALDLLKHEKPDADSVFARIGRRILESGPPPGKWRQEAQVIHRDNWLFTDILVP
                     ISGAPHSWLALEQAMVIAHREAARLHGLHVLSEERNGVTEELQGEFVRRCAEQGLEGH
                     LLVTSGEVAQQISIQARLTDLVVTTLAYPPPPKFLARLDSGFHELIQRCPRPVLAVPQ
                     VVTPLNHALLAYDGSPKADEALFVAAYLAGCWKIPLTVATVFDEKRVAPETLLRAKIY
                     LEDHDVQANYSPETGDVAEAIINASDAHQADLIIMGGYGFNPVMEIILGSAVDQVLRQ
                     ARKPVLICR"
     gene            9551..10363
                     /locus_tag="AC16CB_03430"
     CDS             9551..10363
                     /locus_tag="AC16CB_03430"
                     /inference="COORDINATES: protein motif:HMM:NF046770.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaI-LiaF-like domain-containing protein"
                     /protein_id="HIR3341552.1"
                     /translation="MKRGTLFWGFVLIILGLALLLDNLGILGNIDIWNLLWPLFLIAL
                     GAWILFGRVVRRAPTTEHVSIPLEGAQSARVRVGHGAGRLDVYAGSGLGNLVEGDFTG
                     GLDYRSQLSGDVLEVKMRPPETYFVIVPKYFPFDLSPGLTLDWSFGLTADIPLSLDFE
                     TGASESRLNLRDLRLTELRLKTGASSTSIDLPANAGQTRATIESGAASVNIAIPEGVA
                     ARIRTQGGLSTFEVDTNRFPQVGGVYQSPDYDTATNRIDMDIQMGVGSVKIY"
     gene            10376..11929
                     /locus_tag="AC16CB_03435"
     CDS             10376..11929
                     /locus_tag="AC16CB_03435"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HIR3341553.1"
                     /translation="MKPRQRRMAEGILFTDQYQLTMAQLYYRMGLHEKRVQFDHYFRD
                     YPQYNSHKAGYCISAGLEWLIDWMSEARFGEQEIEYLHSHQSSAGGRLFAEDFLNWLQ
                     QNGNFDGISLQAIPEGRVIHPNEPITVVQGPLAMAQILETSLLNHINYQILIATKAAR
                     IREIGRGQVMLEFGLRRGHGTGANAGARAALIGGADYSSNVGISAIMGYPPKGTHAHS
                     MVQLFIALGMSELDAFQAYADVYPDDCLLLVDTINTLESGVPNAIKVFDNLRKKGHKP
                     VGIRLDSGDLAYLSIQAAKMLDKAGFNDASIVLSNNLDELIIWQIVTQIEEEAPRYGV
                     IPDQLIRRLVYGVGTRLITSWGEPALGGVYKLVAVCDEGVWKPAIKISESISKTPNPG
                     HKQVWRLYDQRGNATADLLSLEDENLKDAKQIVLRHPTDHAKYRVLARDQISEIESLL
                     VEVLKDGKLVVDLPNIEQMRQQRLADVERLDAGVRRIMYPHIYHVSITQELWDLKQKL
                     IKSTMSGIH"
     gene            12011..12649
                     /locus_tag="AC16CB_03440"
     CDS             12011..12649
                     /locus_tag="AC16CB_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341554.1"
                     /translation="MVKWLESRVLWGSLLILAGVVFLLQNLFHIDIGAFFWAVLLAMA
                     GAVFITVFISNRQLWWALIPGFTLLGVSATVFISNYLPRLEGVLGGFFVLGGIGLAFV
                     VVYLNDRRFWWALIPAGVLFTVAIISVVEGLFGGLETGGIFFIGLGLTFAAVAIFPAP
                     QGKMRWAWYPAAVLTVMGLLIAAAAENLINYVWPVALILGGLVLIYRNIASR"
     gene            12659..13126
                     /locus_tag="AC16CB_03445"
     CDS             12659..13126
                     /locus_tag="AC16CB_03445"
                     /inference="COORDINATES: protein motif:HMM:NF015957.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIR3341555.1"
                     /translation="MENKLYRSRTDAMLGGVCGGLAHYLRIDSTLVRLFFILIALGGS
                     GIGVFVYLLLWIIIPLEGQKKDVTLQDTVRSGSEEIAERTRQMGDDLRHLVRNPNPQA
                     GLLIGAALIILGAIFLIQNLDLPWLYWLDFDVIWPALLIVGGIALLMRHWRGE"
     gene            13128..14198
                     /locus_tag="AC16CB_03450"
     CDS             13128..14198
                     /locus_tag="AC16CB_03450"
                     /inference="COORDINATES: protein motif:HMM:NF046770.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF transmembrane domain-containing protein"
                     /protein_id="HIR3341556.1"
                     /translation="MSERKETPHRRPRSLFGPLLLIALGIVFLLNNLGILRGDGWNTV
                     VQLWPLILIVIGLDSLYQRQGLVGAIFIIGLGTVFLLSNFNLLNVNAWQLVLRLWPLL
                     LVAIGLDLIVGRRSLWASLAGLAVLLAILAGVLWLQGVRLGAGQVLSGQEVRQSFQGA
                     TRAEIILQSSAGDVYIHSLPGSNELIAGQVASGKRRRIFEEISMQGEQANYRLREDEN
                     FGYFWGRAGQRTWDLGLTPTIPLEIHYSQGAGVSNLDLSELQVYALRVNMGVGQTTVT
                     LPAKGEFDGRIEGAVGQLIIIVPKGMELRVRSNLALANIITPAAYRKRDRVYTSPGYE
                     TAANRIDLEVGMAVGNVMIREK"
     gene            complement(14240..>16219)
                     /locus_tag="AC16CB_03455"
     CDS             complement(14240..>16219)
                     /locus_tag="AC16CB_03455"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HIR3341557.1"
                     /translation="SYSRHLMVEAGTGTGKSMAYLIPATLWAIQNNNRVIISTNTINL
                     QDQLINKDIPDLRNAMGLDVQAVVLKGRSNYFCPRRLEILRRRSPETSEELRVLAKVL
                     VWLQNTRTGDRAEINLNGPIERAIWSRISAEDEGCTSENCIKRTGGICPFHRARQAAQ
                     SANILIVNHALLLSDVATGNRVLPEYQTLIVDEAHHLEDATTNALSYRVTQWDIERLL
                     RELGGPRGGALGWLLAAAQEVLGPDEFVAFEQLAHHAADLAFQFEHLSRLFFSSLDEF
                     LTEQREGRQVGAYSHQVRIQPATRTLSTWASVEAAWDEAEHTLSPMLDVLAKLLQGLS
                     EILPALPEGDEELFSALSNLYHRFTELHDNMEGMVFKPDQGLIYWAEANTNSSSVALN
                     AAPLEIGPLMQRYLWHEKSAVILTSATLTAAGEFTYLRHRLYAEEADELSVGSPFDYE
                     NAALLFVANDIPEPGDRQGHQRGVESGLIGLCRAIGGRTLVLFTSYDQLRRTSNAISP
                     ILAKNDILVYEQGEGASPHSLLESFRTSERAVLLGTRAFWEGVDIPGEALSVLVIVKL
                     PFDVPSDPIIAARSETFEDPFYEYSLPEAILRFRQGFGRLIRTQSDRGVVAILDRRIL
                     TKRYGRLFIDSLPECTSRVGPLGELPRLAKQWLNL"
BASE COUNT         3852 a         3884 c         4394 g         4089 t
ORIGIN      
        1 tcacccgcgc cctcgccgcc atcaaagcgg gcaagcagca gtatctctat ctgggcaatc
       61 tggatgctaa gagagattgg ggatacgccc cagattacgt ggcggctctg tggaagatgc
      121 tccagcatga ccaagcgggt gattttgtca ttggcacggg cgaggcacat tcggtgcgcg
      181 agttccttga tgaggctttc ggttatatca acatggactg gaaaggatgc gtgcgcatcg
      241 acccgcgcta ttaccgccca accgaagtag attacttgct ggcagaccct gggaaggctc
      301 agcgggagct gggctgggag ccgagggttt tctttagaga cctggtgcgt attatggtgg
      361 atgccgatct cgaattaatg ggtcttaaat ctcctggcga ggggaagcgc attcttgagc
      421 agcgccatgg gaaatggcac cgttgggaaa gtcaagtagt tagcatggag tgatggaaga
      481 gaatagatta tcctctaaat tatatcgagc cctgatcacc ggcggggcag gcttcattgg
      541 atcgcatcta gcagaagcgt tgctatcacg aggacaccat gtcaccgtga tcgacaacct
      601 atcgacgggg aggttcgaga acatccagca tctgatagac cacccgcgct ttcgttttgc
      661 gatcgatacc atcaccaacg aagtggtgat ggatcggctg gtgagtgagt gtgatgtaat
      721 ctttcatttg gcagcagcag tgggtgtcat gttgattgtt gagaaacctg tgcacaccat
      781 agagaccaat gtgatgggca cggaagccgt tctgaaagcc gcattgcgct atcgagccaa
      841 agtattgatt gcatctacct ccgaggtgta tggcaaaggt aaccatatcc catttcgaga
      901 agatgacgat gttgtgcttg gtcctacttc gcgaaaccgc tgggcatacg cggcctccaa
      961 gatggtagac gagtttttag cactcgctta tcatcgcgag aagggtttac cggtagttgt
     1021 ttatcgttta ttcaacaccg tgggaccacg acagacggga cgttatggca tggtgatacc
     1081 gcgttttgtt caacaagccc tgcgcggaga taccctcgag gtttatggcg atgggcaaca
     1141 atcacgatgt tttctgcatg tcacggatgc cgtggatgcg atcttggctc tagcggattg
     1201 ttcggaagcc gtgggagagg tatttaatat cgggtctgtg gaagaaatta cgatcgatgc
     1261 gctggcgcgt aggatcctgg atcgtgcaca gcagattacc aagaagagtg agaatgtctc
     1321 acgccagatc aaatacgtgc cttatgatca agcgtatgcc attggttttg aagacatgcg
     1381 ccaacgcgtc ccagaagtca ccaagatcgc tcaatacaca gggtggaagc caaaacgaag
     1441 cttggagcaa atcctgctgg atattcttga agatactgta aaaaagagta ccgtttgacc
     1501 aaacctcata gaattctcag tatattgggt acccggccgg aggtgatcaa gttagcgcct
     1561 gtcattttgg agatggagcg caagccgggt caatttataa gccgggtgtg cgcaaccgct
     1621 caacaccgcc agatgcttga ccaaactctt gatgtctttg gattgaaagt tcatcatgat
     1681 ctgaatatca tgaccccagg gcagacgata tcacaggtca ctgcccgcgc cgttgaggga
     1741 ctggatcatg tgatctccag cgagcgtccg gatcttgtcc tggtgcaagg tgatactacc
     1801 acggcgttct gtggtgcgct ggcagcatac tatcatcaag tgcgcgtggg tcatgttgaa
     1861 gcaggattgc gtacaggtga taaatatgct ccctatcccg aggagataaa tcgtcgcctg
     1921 attggccagc tggcggattt tcacttcgcg ccgacggaac gggcgcgcca agcacttctt
     1981 ttagaaggat tccctgaggc taatattttc gtcacgggca atacagttgt tgacgccttg
     2041 ctgtgggtgc gtgaacgcgt acgaaaagct gttcctgaat tgcctgatgg catcaatgag
     2101 tggctccagg atagacagat ggttctggtg acaggccatc ggagagagag cttcggcgaa
     2161 ggatttgaga atatctgcca ggcaattcgt caggttgcgg atatcttgcc cgaggtagta
     2221 tttatttacc cggtgcacct caatccgaat gtacgcgagc cagttggtcg cattctaggc
     2281 gggcatgaac gcatccactt gatcgatcct ttaccatatg agcccttcgt ctggctgatg
     2341 gaccgtgcca ctgtagtgct cactgactcg ggcggcatcc aggaagaagc gccttcattt
     2401 ggcaaaccgg ttttggtgat gagggaggta accgagcgcc cggagggtat cgatgcaggg
     2461 gttgcccgcc tggtgggtgt ccgtcaagaa gggattgctg ccagcctgat tgagttacta
     2521 acccatccac aggctcgaca ggcaatggcc gcggtgcaga acccctacgg tgatggcctc
     2581 gcagcgaaga gaatcacaaa tattctggaa agaatgctat aactggcgtt tgctgtttaa
     2641 gtatttcctg tactatttaa tgctctccaa cagcgtgctc acagccgctt cgaggactgc
     2701 atcctcttct cctaaggcac tttcttcagt taccggtaca accacatcgg ggattacacc
     2761 cacgccctcg agggccaact gaccatccgg cattacatac cgcccagttg ggaactgcat
     2821 tttgatatcg ccggggagtg tgtattgccc gcgcccaact tcgccatatg cacctgcact
     2881 agggtagtga cccacgaccg tagagcgtcc attttgagtg agcgcgtaag caaatccctc
     2941 acaagcgctg acgcagtctg ggcccactag cactgcaatt tgccctgcat agaaaagcgg
     3001 tcccggttcg attttactag tcaagccgct tgcctcaaag cgattacttt cttcgctgta
     3061 atagagactt tcataaagag tgatttcttc ttcaaagaag tagccagcaa agtccgtcgc
     3121 cagatgtccc gatccccctg agtttacgcg caggtcaatg attaagccag ggatttcatt
     3181 atcaatgagc cctttgataa agtgttccca aaggcgggcg atcaggctgt aatcggcgga
     3241 gaaagtattg acgcgcacat aacccagacc tgattcgtcc aggatctcgc cctcgactgg
     3301 gataatcagt tcatccttgg acaattcagg gatggctgca aacagggagt cgtaatcgac
     3361 atcggctctc atgcgggctt cttgctcagc gttgctgccc ggattctgga acgttactgt
     3421 aacatttgta ttgggtggca cgcgagttaa gaagagcacc tgtccaattc ggcggttatg
     3481 ttgtgtagaa tatggtccga aataggggtt caccgcatcg atggcttcac tgattggctt
     3541 gccttcccag gttaacaact ctgctccaat ccttatgccg gctcgctccc cagggctgtt
     3601 tgggattaca ttggtcacca atacacgccc atcgctcagt tcagttaagt ttaggccaaa
     3661 acctccgcca ttatcttcaa agaaaatctc tccattgatc tgcatgccga catgcccgtc
     3721 ggggatttcc tgtgtaaagt catgtaaaac tctataaaaa tccttatcat tggatacatt
     3781 agctatgcga gctgcatact catccttaag tgtttcccag gagatgttct tttcccgggt
     3841 aaatgggtat gcgcgctcaa ctttctcgat catggcttga aaggcatccg catagctcat
     3901 tgtggaaaag tcgttgactg cactcgcacc ttcgcttaag gtgatatttg gtcgcgcttc
     3961 tttgtaaaaa ctgaaaggct cctggttgat atccacaagg ttgtaacccg atgggatagg
     4021 agcagcggga tcatcctttg tgaacagcaa gccgtcctct ccaaatccag taggaaagct
     4081 ttgctggtcg tcaggagccc agaccaccag aataccacct acaatttcgt cctctcgatc
     4141 agggtcaacg attgtagaca cataagaagt tgaccatccc ttgccatccc gcctttcaag
     4201 gaaaggtcca ccccaggtat tagaccagta tgcgacggcg aagatttgta cgccgacttc
     4261 gtcctttcca tcgttgtcca catccaattg ggttccctgg ggtatagaag gcaaggccag
     4321 gctgtagctt actctctgat cattgtggat ctccacaggt ccaatggctt gcccttctag
     4381 ccggaagata aactctttat ctctgctggc aaaaccagct tgatcctcca gtagaacgaa
     4441 gggttcaaat atggtgttga tgaaaaatgg cgatgtgtat ggaatgtctc cagtgatgaa
     4501 aaccggctca tccggatttt cttcgcctgg actgacagga acgggaggaa gccgggtcgg
     4561 tatgggggta ggctcgggta tcggggttgg ggtcggggga attgcagtat tggtaagagc
     4621 ttgacagccc aaggcaaaaa gagcaaagac aactgcgaac gcaagtccaa atcttaggtt
     4681 aatgaaggta gagttaaagg ggtttttcat catcggttat cctaatcgaa ataagtatcc
     4741 aattaaagct ccggcgaaaa tcagccaggt tgtattgatt ttgtatttca ccagcaaaaa
     4801 ccctgaaatt agtaacaaaa ggatgcctgc cgggttgttc agagatgagc gtgccaattg
     4861 ccaggttact ccagccatta agcccagtga agcaacattt accccatcta atagactggc
     4921 gagccagggc gagttccgca agcgaggtat ccacacactg ctcgcggcga cgaagataaa
     4981 ggcgggcaaa aagatcgcaa ccgtgcttaa gatggctccc ggaattccgc cgaggagata
     5041 gccaataaat gtggccgacg tgaataccgg gccgggtgtc acctgaccga tggcgatggc
     5101 atccagcagc tgcccttcgg tgagccatcc gagtcgagct acaaagtctg cgtgcagaaa
     5161 agccagtaat acgtagccgc tcccatacaa gacagcacct atcttcagga atgttaagaa
     5221 aagcaagccc aggctgaacg gagcgacgcc caagggtacc agagagcttc cgccaattaa
     5281 tatggcgcag gtcatattat acgaccttaa tcgcttagcg ttctcgaaaa ccatgaccag
     5341 agatccgcaa atgagcaaga tcaagatggg atgcaagttt aaaaaatata gaatgagcgc
     5401 cgcaagtcca gcaaatgctg tctgggtgcc cttgattgcc ttctgcccta aaccccacaa
     5461 tgcctggatt atgatggcga tcacaacggg ctggatgccc ttcatcaacc aaccagattg
     5521 gggtgtggtg ccataattcg catacagcca agcgagaatc aacacgatgc tcatcgccgg
     5581 gaggatgaaa cacgtcccag ccaggaggaa tccaatccac cctgccctcc gtagcccgat
     5641 atggatcacc atctcggtgg agttaggacc tgggataagg ttggttgcac ccaggaggtc
     5701 tagaaagtcc tggtcactga gccatttacg tcgggtaacg acctcgtccc gatacatggc
     5761 gatgtgggcg gcaggcccgc caaatgccgt gaagcctaac ctcaagaata ggccggcaag
     5821 ctcgagcaaa cggaggagac tggtctggtg tggcttcaat ggctcagata ggattaaagg
     5881 gtgtcagtaa taaagcccta agagacgtgt aactgtgaaa acctactttt gtgccttgta
     5941 aaggttctct agctcgaatg acagtgaatc tagttgagcc ataataggtt ctaaatcatc
     6001 acgggtgggg atattctgct tttccaagaa ttgaaccata tcccatataa atggggtggg
     6061 ttgattggag cgccttccaa ttcccaggtg gatcaacttt tcttgcaagc gcttgccttc
     6121 cgtttcagcc agctcgccgc gctggaccaa aatctgtatg cgctgtttga tctcttcgtc
     6181 caccgggttc gatggcccaa tccgtgaagc caggtagcgt tggagcgcgt caaggcgatt
     6241 gttgctgact ttaatcagat tggctaatat cttaaggggg agaaaaccgc ttttcttttc
     6301 cagttcgaag agtatctggg ttagagtgac agccgaaaga tcctcgcggg tggcatggtc
     6361 aatgacctga acttcttcac cctcaaagat gagttcagca ataccttgca aggtgatata
     6421 ttgtttggct tgcgtgttat atagcttgcg atttggataa cgcttgatga tgggcatcgt
     6481 gagctattgt tgttgcgctt ttttcagttc atcaactttc ttagaaagtg tggcaatttt
     6541 gtcgctcagg gatttgatat cctctttgct agggatattc atttgaagta gggtttcttc
     6601 aacgcgcttc gtaacttcgt cttgaaactg ctgtgcctct ttcttgcgct tgtcctttaa
     6661 ttcgcgaatc aactttttcc cgtcctgctc tgcaatctca cctcgctcta ctaagcggtt
     6721 gacaaattct tcgatctcgt cttgggcaag ggcgatagcc cctattccag ccagcaagac
     6781 tttgcgggca gcttcgaaca aaggcttgag ctcctctcct ttagctgatc cctcgacttt
     6841 ttccttcgta ggcatgatta acctcccggt tatactagtc aggaataatt aggttagacc
     6901 ttttgcattg tttatgacgc gctgcgttat aaataatcat aacatgccga ggtacacatg
     6961 tcaaaccgcc aaaagatgtt aaatagacgg aaaaggttat aattcaacca atccctatga
     7021 agatcctctc tgtatccgat gttctcgtgc cgtttattta tagctcacaa gtgagggcta
     7081 agttttctgg agtcgatttt gtcatagctg gtggagacct gccatattat taccaggaat
     7141 ttatcttaag tatgctggat gtgccccttt tttttgtgcg tggcaaccac gacaagctgg
     7201 aggagcacag ccaagtgggc aaacgcagcg ggccggatgg cggtgtcgat atgcatcttc
     7261 gaaccgagga atgtaaaggg atactattgg caggggtgga aggctgcatt cgttacaggc
     7321 taggtgattt tcaatattcc caatctgaga tgtggagaca tgtatggcga cttattccct
     7381 cattgctcaa aaaccgcatc caacatgggc gctacctgga tatttttatc acccacgcgc
     7441 cacctcgcgg catccacgat caggacgact tgcctcacca tggcgtcgat gccttccgct
     7501 ggctgataca gacttttcat ccagcctacc atttccatgg tcacattcac gtctaccggc
     7561 cggatgcgcc agttgaaagc ttggtgggta tgacaaaggt gatcaatacc tacggttttc
     7621 gcgagacctt aattacaaat cagcgacaaa ttaatacagg tttgcgcgcc tgacgtagca
     7681 cctggtccac agcacttcct aggatgatct ccattacagg attaaacccg tagccaccca
     7741 taatgatcaa atcagcctga tgagcgtctg atgcattgat gattgcttct gctacgtcac
     7801 cggtctctgg ggaatagttg gcctgaacat catgatcttc caaatagatc ttggcgcgca
     7861 gcagcgtttc gggggcaacg cgtttttcat caaatacggt ggctaccgtg agggggattt
     7921 tccagcagcc tgctaaatag gcggcaacaa ataaagcctc atccgctttg gggctgccat
     7981 cgtaagccag cagggcatga tttagcggag tgacaacttg agggactgcc aatactggtc
     8041 tgggacaacg ttgaatgagt tcgtgaaagc cagaatctag ccgggcgagg aatttgggcg
     8101 gaggcggata tgctaaggtg gtaactacaa gatcggtcaa tctggcttga atggatatct
     8161 gttgggcgac ctcaccggat gtaacaagta gatgcccttc caagccttgc tcggcgcaac
     8221 gccgcacaaa ttcaccctgt aactcctccg taactccatt tcgctcttcg gagagtacgt
     8281 gtagcccatg caatcgggca gcttcgcggt gggcgatcac catagcctgc tccagggcaa
     8341 gccagctgtg gggagcgcca cttattggaa ccaggatgtc tgtgaacaac cagttgtcgc
     8401 gatgaataac ctgtgcttct tgacgccatt tacctggggg agggccgctc tcaagtattc
     8461 ttctgccaat tcgtgcgaag accgaatcag cgtctggttt ctcatgcttg agcaagtcta
     8521 gcgcggctac ttcggtttta atctccactc cccattcatt ctccaaagcc gcacgatgtt
     8581 cggcgatcca taaatatagg tcggtttccg tgcggtcggg aaaatttcgc aggataccac
     8641 cttcttgaat tgctcttata gcaggcaaat agattgaatc gtaccagtca gctactgcct
     8701 catcgtttgg gataaatcgt tgttgatcct gccccatata atgacggtga actgcgatat
     8761 gctcctccag aacggggtat tgcccgggta tcgtcacact taaatccgcc tcaggtcgca
     8821 attcatctag gtgggtgcgc tctaagaatc cagcatattc tgccttcaaa atcaggtcat
     8881 ccggctgagt atctggatca agagggactc tcacaagaac ctcagtgacg ttggcctcga
     8941 tggttttaga tccgtactgg cgagcaatag acactcggtg gttgccgtct tggacgaaat
     9001 aggcttcacc aatcttataa acgtcgatgg gcggcaatcc gcgggaggcc tcggaagccg
     9061 ccttgaccct tgcccaacga tcctgggtga agtcctgacg agggagaaaa tccctcgtaa
     9121 agtcattgta acgacctaca cttcctacta tggcatccaa ggggatatcc tgaaggccgc
     9181 ggttcgagct ggcatacgcc ctgagcttct gccgtacttc ttcgtaggac agtagttcag
     9241 tggattcacc cgtgacgcgc gcaaccaaat ccttaagttt tgcctggcta cgggcacgac
     9301 ggaaatcctg taaggaggtg atatagcggt aggtatctag gtttcgttga tttggatcgt
     9361 aattcacgct aatattttaa tacgaaacca gccgatggag gtaaattaca tctttatcgg
     9421 ggtattcagg tggattttat taacaatttc cctgctaaat aataggtgta gaatacagat
     9481 atagattttg tgtgtctaac acgcgacagg atccaccttt tctacgtatt aatacaagga
     9541 gtaaacgaac atgaaaagag gcactctttt ttggggattc gtattgatca tcctaggttt
     9601 agctttgctt ttagacaatc tgggtatatt ggggaacatc gatatctgga atttgctctg
     9661 gccgttattc ctgatcgccc tgggggcttg gattttgttc ggtcgggtgg tcaggcgcgc
     9721 tccaacaacc gaacacgtca gcatacctct cgaaggtgca cagagcgcac gggtacgcgt
     9781 ggggcacggg gctggaaggc tagatgtata cgctgggtca gggttgggta atctggtgga
     9841 gggtgacttc accggaggac tggattatcg cagccagctc tcaggagatg ttttggaggt
     9901 caagatgcga ccgccagaaa cctatttcgt gatcgttcca aagtatttcc cgtttgattt
     9961 gtcgcccggt ctaacattgg actggtcgtt tggacttacg gctgatattc ctctgtcgct
    10021 ggatttcgag accggggcaa gtgaaagccg attgaattta cgcgatctcc gcctgacgga
    10081 gctacgcctc aagactgggg ctagctcgac atcgatcgat cttcctgcca atgcagggca
    10141 aacgcgcgcc actatcgaaa gcggagctgc ctcggtgaat atcgcaatcc ccgaaggcgt
    10201 agccgcgcgc atccgtacac aaggtgggct ttccacattc gaagttgaca ccaaccgttt
    10261 cccccaagtg ggcggcgtat atcaatctcc agattatgat accgccacca ataggatcga
    10321 tatggatatc cagatggggg ttggatctgt caagatttat taatcaggtt ttgctatgaa
    10381 accaagacaa aggcgtatgg ctgaaggtat ccttttcaca gaccagtatc agctaaccat
    10441 ggcgcagcta tattaccgta tggggctgca cgagaagcgt gtccagtttg accactactt
    10501 ccgagattat ccccaatata actcgcacaa agcgggttac tgcatcagtg ccgggctgga
    10561 gtggttaata gattggatga gcgaggcgcg atttggcgag caagagattg aatacttgca
    10621 tagccatcaa agtagcgcag gcgggcgcct gtttgcagaa gattttctga attggctaca
    10681 gcaaaatggc aattttgatg gcatcagctt gcaggcgata ccagaagggc gcgtgataca
    10741 cccaaatgag ccgatcacgg ttgtccaggg tccgcttgcg atggctcaga ttttggagac
    10801 ttcgctgctc aatcatataa attaccaaat cctgatcgcc accaaagctg ctcgtatccg
    10861 ggagattgga cgggggcagg ttatgctgga atttggcctg cgtcgtgggc atgggaccgg
    10921 agcgaacgcc ggggcacgcg ccgccttgat tggcggtgct gactattcat ctaatgtggg
    10981 gatttcggcg atcatgggct acccgccaaa gggtacccat gcgcacagca tggtgcaact
    11041 cttcatcgcg ttgggtatga gcgaactgga tgcatttcag gcttacgcag acgtctaccc
    11101 cgatgattgt ttgcttttgg tagataccat caacacgctg gagagcggtg tacccaatgc
    11161 aattaaggtc tttgataatc tgcgcaaaaa ggggcataag ccggtaggca tccgcctgga
    11221 ttcgggtgac ttggcttacc tctccatcca ggctgccaag atgctggata aggccggctt
    11281 taacgatgca tccatcgtgc tctcgaataa tctggacgag ttgatcattt ggcagatcgt
    11341 cacacagatt gaagaggagg cgccccgtta tggggttatc cctgaccagt tgatccgcag
    11401 gctggtgtat ggggtgggca cgcgcttgat cacctcttgg ggcgaaccag ccttgggtgg
    11461 ggtttataaa ctggtagctg tttgcgatga aggggtgtgg aagccggcta tcaagatttc
    11521 cgaatcgatc tcaaaaacgc caaacccagg tcataaacag gtctggcggc tgtatgacca
    11581 gcgtggcaac gctaccgccg atctgctcag cttggaggat gaaaatctga aggatgcaaa
    11641 acagattgtg ttgcgccacc ctacggatca tgccaagtac cgggtgctgg cgcgcgatca
    11701 gatctccgag attgaatcgt tgctggtcga ggtcttgaaa gatggaaaac tggtcgttga
    11761 cttgccaaac atcgagcaaa tgcgccagca gcgcttagcg gatgttgagc gcctggatgc
    11821 cggtgtccgc cgcatcatgt atcctcacat ctatcacgtc tcaataaccc aggaattgtg
    11881 ggatctaaag caaaaattga tcaaatcgac gatgtctggc attcattagt gcaagtagat
    11941 gcgcagttga agaaggtctc tccaactgta tatcttctca ataaatttcg taggatcgag
    12001 gaggatgatc atggtcaaat ggctggaatc tcgtgtcttg tggggatcat tattgatcct
    12061 ggccggagta gttttcttac tgcaaaattt attccatatt gatattggcg catttttctg
    12121 ggcagtattg cttgccatgg cgggcgcagt attcattact gtgtttatca gcaaccgtca
    12181 actgtggtgg gctttgatac caggttttac tcttttgggc gtttctgcca ccgttttcat
    12241 aagcaactat ttacccagat tagagggtgt cctgggtggt ttctttgtct taggtggaat
    12301 tggtttggct tttgtcgtag tctacctgaa cgaccggcgt ttctggtggg cattgatccc
    12361 ggcgggggtg ttatttaccg tggctataat cagcgtggta gaaggtttgt ttggaggatt
    12421 ggaaacaggc gggatatttt tcatcgggct ggggttgact tttgccgcgg ttgctatttt
    12481 tcctgctccg caagggaaga tgaggtgggc atggtaccca gccgcagtgc tgacagtgat
    12541 gggtttgctg atcgcagccg cggctgagaa cctgattaat tacgtttggc cggtagcttt
    12601 gatcttgggg ggtcttgtcc tgatatatcg caatatcgca tctcggtgaa ggagtgaaat
    12661 ggagaacaaa ctatatcgca gcagaacgga tgcaatgctt ggtggtgttt gtggaggcct
    12721 ggcacactac ctgcgcatcg attcaaccct ggtgcgcctg ttttttatcc tgattgcgtt
    12781 gggaggcagt gggatcgggg tttttgtcta cctgttactg tggattatca ttcctttgga
    12841 aggtcaaaag aaggatgtga ctttacagga tacagttcgc tctggcagcg aagagattgc
    12901 cgagcgaaca cgccagatgg gcgatgatct gcgccatctt gtgcgtaatc ccaatccaca
    12961 ggctggcttg cttattggcg cagcattaat tattttgggt gcgatcttcc ttatccaaaa
    13021 tcttgatctg ccctggttgt attggttgga ctttgatgtg atttggccag ccttgttgat
    13081 tgttggaggc attgcactgc tcatgcgcca ttggagagga gaataagatg agcgaaagaa
    13141 aagaaacgcc gcaccgcagg ccaagaagcc tgtttggtcc attgttatta atcgccttgg
    13201 ggattgtatt cttgctcaat aacttgggca tactccgggg tgatggatgg aatacagtag
    13261 tgcaattatg gccgctgata ttgattgtca ttggcttaga cagcttatat cagcgacagg
    13321 ggctggtcgg tgcgatattt atcatcggct tgggcacggt cttcctgctg tctaacttta
    13381 atctgctcaa tgttaatgcc tggcaacttg ttctgcggct ctggcctttg ctattagttg
    13441 cgattggctt ggatctgatc gtgggcaggc gctcgctgtg ggcatctcta gcgggtttgg
    13501 ctgtactgct ggctattctg gcgggggtct tatggctcca gggtgtgcgg ctgggagcag
    13561 gacaggtttt gtcgggtcaa gaagtgcgcc agtcttttca gggagcaaca cgcgccgaga
    13621 tcatccttca aagtagcgca ggcgatgtct atattcattc tttacctggc tcgaatgagc
    13681 tgatagctgg ccaggtggca tctggaaaac gcagacggat atttgaagag atatctatgc
    13741 aaggggagca agccaattac cgtttgcgcg aagatgagaa ttttggatat ttttggggac
    13801 gcgctgggca gcgtacctgg gatctgggct tgactccgac aattcccctc gaaattcact
    13861 acagccaggg ggcgggggta tccaacctgg acttgagcga gttacaagtc tacgccttaa
    13921 gggtcaacat gggtgtaggc cagaccactg tcaccttgcc agccaaggga gagttcgatg
    13981 gcaggattga aggagctgtc gggcagctga ttatcattgt gcctaaagga atggaattga
    14041 gagtgcgctc caatctggct ctggctaata taatcacccc ggctgcttac cgaaagaggg
    14101 acagagtata tacctcccct ggatatgaga cagcggcgaa tcgcatcgat cttgaagtcg
    14161 gaatggctgt cgggaacgtg atgatccggg agaagtaata acctgaaatg aattagagaa
    14221 gcaacaaaac tcgtcttctc tataaattca accactgttt tgccagcctg ggcaattctc
    14281 ccagcgggcc aacacgcgag gtgcattctg gcagggaatc tatgaaaagg cgcccatagc
    14341 gtttggtcag gatgcgccta tccagtatgg caaccacacc acggtcactc tgggtgcgga
    14401 tcaggcgccc aaaaccctgg cggaagcgca agatcgcctc tggcaaggaa tattcgtaga
    14461 atggatcctc aaaggtctcc gagcgggcgg caataatcgg atcggaaggc acgtcaaagg
    14521 gaagcttgac aattaccagt acagacaggg cttctccggg gatatccaca ccctcccaaa
    14581 aggcacgcgt acccaataat acggcacgct cgctagtgcg gaaactctcg agcagactat
    14641 ggggtgaggc tccctcgcct tgctcgtaaa ccaggatgtc attttttgct aatattgggg
    14701 atatggcatt tgacgtgcgg cgaagctggt cgtaagaagt gaaaagtacc agcgtccgcc
    14761 cgccgatggc acggcacagt ccaatgagcc cgctttccac gccacgctgg tgaccttggc
    14821 ggtcgcctgg ctcggggata tcgttagcca cgaataagag agctgcattt tcataatcaa
    14881 atggcgaacc tacggagagc tcatcggcct cctcggcgta caagcggtgt cgtaagtagg
    14941 tgaactcccc ggcggcagtc agtgtggcag atgtgagaat cacagcggat ttttcgtgcc
    15001 atagatagcg ctgcataagc ggaccgatct ccaggggtgc ggcgttcaaa gcgacgctgg
    15061 agctatttgt gttggcttct gcccaataga tgagcccttg atccggcttg aagaccatcc
    15121 cttccatgtt gtcgtgcaat tcagtgaaac ggtgatacaa gttgctaagc gcgctgaaaa
    15181 gttcctcgtc cccttctggc agggctggca aaatctctga tagcccttgg agtagcttgg
    15241 cgagcacgtc aagcatggga gagagcgtgt gctcggcttc gtcccaggca gcttcaacgc
    15301 ttgcccaggt agaaagggtg cgtgtggcgg gttggatgcg cacttggtgg gaataggcgc
    15361 ctacttgacg gccttcacgt tgctcggtga ggaattcgtc taaggaacta aaaaagaggc
    15421 gcgagaggtg ctcgaattga aaagccagat cggcggcatg atgggctaat tgttcgaagg
    15481 cgacaaactc atctggtcca agtacctcct gggcagccgc cagcagccag ccgagcgcac
    15541 cacctcgcgg tcctcccaat tctcgcaaca ggcgctctat atcccattgg gtgacgcgat
    15601 agctcaaggc attggtcgtt gcatcctcca ggtggtgggc ttcgtctacg attagggttt
    15661 gatattcggg taagacacgg ttaccagtag ctacatccga aagcagtaga gcgtggttga
    15721 cgattaagat attggctgat tgagccgcct ggcgcgcccg gtgaaatggg caaatgccgc
    15781 ccgtgcgctt gatgcaattc tcggaggtgc agccttcatc ctcggcggag attcgactcc
    15841 atatggcgcg ctcgatggga ccgttcaagt ttatttcagc tcggtcacct gtgcgagtat
    15901 tttgcagcca gaccagcact tttgccaaaa cacgcaattc ttcgcttgtc tctgggctac
    15961 gacgccgcag aatctctaac ctgcgcgggc aaaagtagtt cgagcggccc ttcaaaacca
    16021 cggcttgcac atccaacccc atcgcgttgc gcaagtctgg aatgtctttg ttgatgagct
    16081 gatcctgcag gttaatggta ttggtagaaa tgatgacgcg gttgttgttc tggatagccc
    16141 acagcgtggc aggtatgaga tatgccatgc tttttcccgt cccggtgcca gcttcaacca
    16201 tcagatgcct gctatagga
//
LOCUS       DBNGSX010000100         2483 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_422955, whole genome shotgun sequence.
ACCESSION   DBNGSX010000100 DBNGSX010000000
VERSION     DBNGSX010000100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_422955"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1369
                     /locus_tag="AC16CB_03460"
     CDS             <1..1369
                     /locus_tag="AC16CB_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3341558.1"
                     /translation="YYEKELSFINSRSYGPGRYDPTYEQEGQDYPIGYVRWTEGRNME
                     AFVELLASSRLDVKPLITHRFPIERAPEAYELITGKAKEPFLGVILTYPGASESSISL
                     SSAPASRIIQYEVREAGQVSSVKLGVLGAGNFASAVMLPVLKKMAGIEMLGVASASGV
                     SAAQAARRFGFRYATSQESDVLSDPSINTVAILTRHHLHASQVISALDVGKNVFCEKP
                     LALTREELEDIAATLVRINEKQDEVVPLLMVGFNRRFSPMAKKLQAFLADRHEPLVAH
                     YRVNAGYIPPTHWVQDPAQGGGRIIGEGCHFVDFLTYLVGAPPVSVVGQAMSDNGHYQ
                     GDNVVLTFTFADGSVGTLTYLANGDKAFPKERVEVFAEGRVAVLDDFRALEMVRDGKR
                     DSYRSRLRQDKGHAGEWQALIAAISAGGPPPIPYDHIIGVTQATFAAVEALRNNERRL
                     VKFQE"
     gene            1366..>2483
                     /locus_tag="AC16CB_03465"
     CDS             1366..>2483
                     /locus_tag="AC16CB_03465"
                     /inference="COORDINATES: protein motif:HMM:NF028199.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HIR3341559.1"
                     /translation="MRSVSKLEIAIKALRELGLGQVSQYAWYKILLRTGYIRWATRNP
                     ALKTAGARRDQSRPRGLSGVRPLFRLPAREQVMLTLGESGLADLLKEADEILSGQVRL
                     FGGEPQPLNLSPPGPLLHWTRYDPGRGEGGDAQDIKFLWEPGRFGWAYILGRAYYLSQ
                     DERYAEGFWKFTETFWEANPPYLGPHWVSAQEVALRLLALVFAYQIFSPSIHTTTPRS
                     VLIYQIIADHARRIPPSMTYARAQNNNHLLSEAVGLYTAGLALPNHPKAVRWQKMGWR
                     WIQRGLQKQIAEDGAYIQHSTNYHRLMLQLALWVHALPETQRQPFPAATRQSLSAATR
                     WLLELVDGESGRVPNLGPNDGAYIQPLTVCPFHDFRPVL"
BASE COUNT          525 a          654 c          721 g          583 t
ORIGIN      
        1 ttattatgag aaggagcttt ctttcatcaa ctctcgttct tacgggccag ggcgctatga
       61 cccaacttac gagcaggagg gacaggatta tcccataggc tacgtgcgct ggaccgaggg
      121 gcgaaacatg gaagcttttg tcgaactgct agcgtcatcc cgcctggatg taaagccttt
      181 gatcacacat cgcttcccta tcgagcgtgc cccagaagcc tatgagctga tcactggcaa
      241 agccaaagag ccattcctag gcgttatttt gacctatcca ggagcttcag agagcagtat
      301 ctccctttca agtgctccag cctcgcgcat catacaatac gaagtgcgtg aggcagggca
      361 agtaagcagt gtgaagctgg gggtattggg agctgggaat ttcgcctcgg cggtgatgct
      421 gcctgtgctc aaaaagatgg caggtatcga gatgctgggc gtggcctcgg catccggcgt
      481 cagcgcagcg caggcagcca ggcgctttgg ttttcgatat gctaccagcc aagagagcga
      541 cgtgctcagc gatccttcga tcaatacggt ggctattctg acgcgccacc acctgcatgc
      601 ctcgcaggtg atttcggctc tagatgttgg aaagaacgtc ttttgtgaaa aaccgctggc
      661 tttgaccaga gaggaattag aagatatcgc cgcaactctc gtgaggatta atgaaaagca
      721 ggatgaggtg gtacccctgc tcatggtagg gtttaaccgc cgtttttccc caatggctaa
      781 gaagctgcaa gccttccttg cggaccgcca cgagccattg gttgcgcatt accgtgtcaa
      841 tgccgggtac atccccccga cgcattgggt gcaagatcct gcccaaggag gcgggcgcat
      901 catcggggag ggctgccatt ttgtggactt tctcacctat ctggtcggcg cgcccccagt
      961 ttcggtagta ggccaagcta tgtcggataa tggtcattac cagggggata acgtcgttct
     1021 aacctttacc tttgccgatg gctcagttgg gacattgact tacctggcca acggcgacaa
     1081 ggcttttccc aaggagaggg tggaagtgtt cgctgaaggg cgtgtggctg tgctggacga
     1141 ttttcgcgcc cttgagatgg ttcgggatgg aaaacgggat agctatcgat ctcgcttgcg
     1201 gcaggataaa ggccatgccg gggagtggca ggccctcata gccgccattt ccgctggtgg
     1261 cccgccccct attccttacg atcatattat tggggtgacg caggcgacct tcgccgcagt
     1321 cgaggccttg cgaaacaatg aacgcaggtt ggtaaaattt caggaatgag gtcggtttct
     1381 aagctagaaa ttgcaataaa agcgttgcgg gagctgggtc taggtcaggt gtcgcagtat
     1441 gcctggtaca agatcctgct gcgaacgggt tatatccgct gggcgacgcg caatcctgct
     1501 ctaaaaacgg cgggtgctcg ccgtgatcaa tctcgtccgc gcggtttgtc cggtgttcgc
     1561 ccattattcc gcctgcctgc aagggagcaa gtgatgctga ccttgggtga gagtggtttg
     1621 gcggacttgc taaaggaggc ggacgagatc ctttctgggc aggttcgatt gtttgggggt
     1681 gagccgcagc cattgaattt gagcccaccg ggccctttgc ttcactggac tcggtatgac
     1741 cccgggagag gggagggggg agatgcacaa gatataaagt ttctctggga gccagggcgc
     1801 ttcggctggg catatattct ggggcgtgcc tactacctca gccaggatga gcgatatgcc
     1861 gaaggttttt ggaaatttac ggagacattt tgggaggcca acccgcctta tttgggtccg
     1921 cattgggtct cagctcaaga ggtcgctttg cgattgctcg cattggtctt tgcgtaccag
     1981 attttttctc cttctatcca caccacaacc ccgcgctctg tgctcatcta ccagataatc
     2041 gctgaccacg cccgacgcat accgcctagc atgacttacg cccgggcgca gaataataac
     2101 cacttactca gcgaggcggt tggcttgtac actgctgggc tggctctccc caaccaccct
     2161 aaagcagtgc gctggcagaa gatgggctgg cgctggatac aaaggggctt gcaaaagcaa
     2221 atcgctgaag atggcgctta tatccagcac agcacaaatt accatcgttt gatgctgcaa
     2281 cttgccctgt gggtgcatgc ccttcctgaa acccaaaggc aacccttccc ggctgctacg
     2341 cgccaaagcc tgtcagcagc cacccgctgg ttgctggagc tggttgatgg tgaaagcggg
     2401 cgcgtcccca atttaggacc gaacgatggc gcctatattc aacctctcac tgtttgccct
     2461 ttccatgatt tccgcccggt gct
//
LOCUS       DBNGSX010000101         6360 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_284233, whole genome shotgun sequence.
ACCESSION   DBNGSX010000101 DBNGSX010000000
VERSION     DBNGSX010000101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6360)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6360)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6360
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_284233"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="AC16CB_03470"
     CDS             complement(<1..939)
                     /locus_tag="AC16CB_03470"
                     /inference="COORDINATES: protein motif:HMM:NF017346.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HIR3341560.1"
                     /translation="MKEIKGIAASPGIAIGPIFVYRPAELVIHNLQAEDASLEWERYQ
                     NAAQKALLELVALRQKVASEVGEEDAAIFDAHQMILRDPDLEEELRAELDTGATAESA
                     TEVVFARQVKSFEEIDDPLFRERAADFSDVGRRVLCILTGVQDNALAALDAPAIVVAQ
                     DLMPSDTAQMNKAMVRGFCTAGGGPLSHTAILARNLGLPAVVGAGKDVLDLKNGDWLV
                     VDGGAGVVVIGADEQQCNQYQQEADRQAKERAIAQAHAHEPAITKDGRQIEVVANVGL
                     PGEEVDALQAGAEGIGLLRTEFLFVERRVAPSEEEQY"
     gene            complement(936..1202)
                     /locus_tag="AC16CB_03475"
     CDS             complement(936..1202)
                     /locus_tag="AC16CB_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR3341561.1"
                     /translation="MSEIELIVRNKVGLHARPAVLFVQTAQGFQSDIRVLKDGKEGNA
                     KSFKAILALAVNQNDKVVVRAEGPDAEQALIALTKLVENNFGEL"
     gene            complement(1199..2464)
                     /locus_tag="AC16CB_03480"
     CDS             complement(1199..2464)
                     /locus_tag="AC16CB_03480"
                     /inference="COORDINATES: protein motif:HMM:NF023401.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 4 glycosyl hydrolase"
                     /protein_id="HIR3341562.1"
                     /translation="MAEKIKLAVLGGSSVATPQLIQALSAQVNRPPMEVALLGRTASK
                     LEAVAAVSARLASRSSVPLQVTHSTDLQRGLEGAGYILNQVRVGGYAARAYDESFPQS
                     FNLPGEETYGPGGMNNALRTIPVTLKHCQIIEDVAPEALLVNLTNPSSFIQYAITKYT
                     NVQVVGVCDSPVWLARSLASLLGAPPGEIWVGYVGMHHFGWVTEVRWRGRDVMGDVLA
                     RLDDHSGLPVDLDFVRAIGAIPTSYFKYYYHPNRMLDKQRGQKTRAEQLMELEEKILA
                     EYEVADLESVPDSLQARGAHWYDEIVVPVLLAHANNAQAVFTLNVVNATALPWMPPEA
                     IIELPVVVTRQGFYPLQLPAAPLDIQAMVRVNAAFEMLWVEAVVERSYEKALRAMMLN
                     HLVQNLDQARAILKEIWPAASGPLSEVHL"
     gene            complement(2464..3648)
                     /locus_tag="AC16CB_03485"
     CDS             complement(2464..3648)
                     /locus_tag="AC16CB_03485"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016874304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR3341563.1"
                     /translation="MTKIDLTLPVEIPEWLQIARRPVEVGEGHLERQYQEIADEVRAA
                     VDAVLPTGKYTLGPHLAQFEKDFAAYCDCKYSIGISSGTAALHLALEALDIGPGDEVI
                     TVSNTYVATAFAVSYTGATPVFVDVDPKTFNMTAELVEAKITPRTRAIIPVHLYGHPV
                     DMDPIMELAKQHDLWVLEDAAHSHGARYKGRRTGSLGHIAAFSFYPSKNMGAYGDGGA
                     ITTNDPELDQKVRVLRYVGQRMKFVHEVIGFQERLDELQAAILSVKLKRLDEWNDRRR
                     SLAVIYDEMLGNTPLTLPYEAGYAHHVYYSYATLAPSEEERRELMIYLYEHGIGTFAM
                     YPTLVPMQRAYAHLGYRASDFPVSAPYANRVLNLPMFETLREDEARRTCEAILAFYEN
                     KI"
     gene            complement(3635..4942)
                     /locus_tag="AC16CB_03490"
     CDS             complement(3635..4942)
                     /locus_tag="AC16CB_03490"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HIR3341564.1"
                     /translation="MAQPSIEKLTVIGGGSSYTPELIDGFIQNEDKVRVGEIVLFDVD
                     QERLKIVGGMAQRMAQYAELETKITLSHDRPASVEGAQFVLSSMRVGQMPARIRDERI
                     PLKYGVIGQETTGPGGTLKAWRTIPVTLDVARDMARYAPESWYLNFTNPSGIITEAIL
                     KHTDIKVVGLCNNPINTQAGMAEFFGVEPQDVFLEWVGLNHVNWVRRVYVRGKDVTSD
                     IMAGLEGLSQLEDLPHFDPELVRTLGVLPTYYLEYYYNHPRKLSEARTSEKTRGEVVR
                     EVEEALMKKYADPNQVVVPPELSKRGGARYSEAAINLILSLMLDRRDIQIVVTRNGTS
                     LPDLPPDASVEVPCVVGSHGVTPLSMGPLPETIRSLCQQAKAWESATVKAGVSGSRRD
                     ARLAMLLNPLVPSYEVAAALVDEMLEANRDYLPQFYGDKNDKN"
     gene            complement(4915..5280)
                     /locus_tag="AC16CB_03495"
     CDS             complement(4915..5280)
                     /locus_tag="AC16CB_03495"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014326.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HIR3341565.1"
                     /translation="MTDELEWEQTLFNLILCAGNARSKAKEAAEHARTGEWDEAQAAL
                     DEANEEQLKAHQINADIIRLEASDQAVPFSVLLVHAMDLLLLAWSELDYTEQFIKMYQ
                     RMDALEAEVAQWRNQASKS"
     gene            complement(5277..5906)
                     /locus_tag="AC16CB_03500"
     CDS             complement(5277..5906)
                     /locus_tag="AC16CB_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR3341566.1"
                     /translation="MQPTLQLILVRHGETEWTELGLLHGRLDSPLSPTGRQHAKQTAD
                     RLRGETFDALFASPKGRAMQTALILGEALGMEPIALDGLSEMDFGWSEGKPLTHFDPD
                     GTGFWLFRPLVRFAMRLTAERTQHFAARIREAIAAMQAQYPNGRLLVVTHWGVISMLM
                     ALLLDGDLQRWRDYGPWEACSISELHAEDGVWHSIRMNEHVHLQEERSI"
     gene            complement(5914..>6360)
                     /locus_tag="AC16CB_03505"
     CDS             complement(5914..>6360)
                     /locus_tag="AC16CB_03505"
                     /inference="COORDINATES: protein motif:HMM:NF015722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HIR3341567.1"
                     /translation="KANHPVVQITWDSAFAYTQWAGKRLPTELEWEKAARSTDGRVWP
                     WGNVFDPRKCNTSSEGTTPVGAYSPGGDSPYGLWDTAGNVWEWIGGKPSPLRMPLRGG
                     DWLDGPDEAQTFFRRMHTPKRKNDFMGFRCAADNALPMETAASGGA"
BASE COUNT         1329 a         1912 c         1752 g         1367 t
ORIGIN      
        1 atattgttct tcttcgctgg gggcaacccg gcgctcgaca aaaaggaact cggtgcgcaa
       61 caaacctatg ccttcagcgc cagcctgcag ggcgtcgacc tcttcaccgg gcaggcccac
      121 attggcgaca acctcaatct gccgcccatc tttcgtgatg gcgggctcat gtgcgtgcgc
      181 ttgcgcgata gcgcgctctt ttgcctgcct gtccgcctcc tgttgatact ggttacattg
      241 ctgctcatct gccccgataa ccaccacccc agcaccccca tccacaacca accaatcccc
      301 attttttagg tcgagcacgt ctttgcctgc gccaactact gctggcaggc cgaggttgcg
      361 cgccaggata gcggtatgcg acagcggccc gccgcctgcc gtgcaaaaac cacgcaccat
      421 ggctttgttc atctgggctg tgtcggaggg catcaagtct tgagcgacca caatcgcggg
      481 tgcatccaga gcggcgagcg cattatcctg cacgccggtg aggatacaca gcactcgccg
      541 gcccacatcg gaaaagtcag ctgcgcgctc tctaaacagc gggtcgtcaa tctcttcgaa
      601 actttttacc tgccgggcga agaccacttc agtggcagat tctgccgtgg cgcctgtgtc
      661 taactcggca cgcagctctt cttcgaggtc gggatcgcgc aggatcatct ggtgggcatc
      721 aaaaattgcg gcgtcttcct cgcccacctc agaagccact ttctggcgta aggcaaccag
      781 ctcaagcaaa gccttttgcg ctgcgttctg gtatcgttcc cattccaggg aggcgtcttc
      841 cgcttgaaga ttgtgaatga ccagttcagc cggacggtaa acaaaaatcg ggccgatggc
      901 gataccaggc gaggcggcaa ttcccttaat ttccttcaca gctctccaaa gttattttcg
      961 actaacttgg tcaaggcgat cagggcttgc tcagcgtccg ggccttctgc acggaccacc
     1021 actttgtcgt tttgattgac ggcgagagcc agaatcgcct tgaagctctt ggcgtttccc
     1081 tctttgccat ctttgagcac acgaatatct gactggaagc cctgtgcggt ttgaacgaac
     1141 agtaccgccg ggcgggcatg caagcccact ttgttgcgaa cgatcagctc gatctcgctc
     1201 ataaatgcac ctccgataac ggaccactcg ctgcgggcca gatctctttc aggatagccc
     1261 gcgcctggtc gaggttttgt accaggtgat tgagcatcat ggctcgcaag gctttttcgt
     1321 atgacctctc caccacagcc tcgacccaca gcatctcaaa agccgcattt acacgaacca
     1381 tagcctgaat atccagcggc gcagcgggaa gctgaagagg gtaaaatcct tggcgcgtga
     1441 ccacgaccgg cagctcgatg atggcctccg gcggcatcca gggcagcgcg gtggcattga
     1501 cgacgttcaa ggtaaaaaca gcttgcgcat tattggcgtg cgccagcagt acgggcacca
     1561 cgatctcatc gtaccaatga gcgccacgcg cctgcaggct atcaggcacg ctttccaggt
     1621 cagcaacctc gtattccgcc aaaatctttt cttctaactc catcaactgc tcggcacggg
     1681 tcttttgccc gcgctgttta tccagcatgc ggttgggatg atagtaatat ttgaaatacg
     1741 aagtggggat agcgccgatg gcgcgcacga agtccagatc gacgggcagc cctgaatgat
     1801 cgtccaggcg cgcaaggaca tcacccatca catcacggcc gcgccagcgc acctcggtca
     1861 cccaaccaaa gtgatgcatg cctacataac caacccatat ctctcccggc ggcgcgccca
     1921 gcaagcttgc caggctgcga gccaaccaaa ctggcgaatc gcaaacaccc accacctgga
     1981 cgttcgtgta cttcgtaata gcatattgta taaagctgct tgggttggtc aaattgacga
     2041 gcagcgcttc aggcgcgacg tcttcgataa tctggcaatg cttcaatgtt accggtatag
     2101 tgcgcagggc attattcatt ccacctgggc cataagtctc ctcgccgggc aggttaaacg
     2161 attgcgggaa agattcatcg taggcccgcg ccgcgtaacc gcccacccgc acctggttga
     2221 gaatgtagcc cgcgccctct aaaccgcgct gtagatcggt ggaatgggtg acttgaagcg
     2281 ggacagatga gcgggaggca aggcgggcgc tgaccgccgc cacagcttcc agcttggaag
     2341 ctgtgcgccc aagcaaagca acttccatcg gcgggcgatt gacttgggca ctcaaggctt
     2401 ggatgagttg cggcgtggcg acgctgctgc cacctaacac cgccagcttg attttttcag
     2461 ccatcagatc ttgttctcgt aaaacgccag gatggcttca cacgtacgcc gcgcctcatc
     2521 ctcgcgcagg gtctcgaaca tcggcaggtt gagcacgcga ttggcgtaag gcgcgctgac
     2581 cggaaaatca ctggcgcggt agcccagatg agcataggcg cgctgcatag gtaccagcgt
     2641 ggggtacatg gcaaacgtgc cgatgccatg ctcgtaaaga taaatcatca gctcgcgacg
     2701 ctcttcctcg ctgggtgcaa gcgttgcgta agaatagtag acatggtggg catagcctgc
     2761 ttcgtaaggg agtgtcaatg gagtgttccc cagcatctcg tcatagatga cagccaggga
     2821 acgccgccgg tcgttccact cgtccagccg tttgagcttg acgctgagga ttgcagcctg
     2881 caactcgtcc aagcgctcct ggaatccaat cacctcgtgg acaaacttca tgcgttggcc
     2941 tacgtagcgc aggacgcgca ccttttggtc cagctcgggg tcattggttg taatcgctcc
     3001 cccgtcccca tacgctccca tgttcttgct gggatagaag gaaaaggcgg cgatgtgccc
     3061 cagtgagccc gttcgccgac ccttgtagcg ggctccatgg gagtgtgcag cgtcttcgag
     3121 cacccagaga tcgtgctgtt tggcaagctc cataatcggg tccatatcca cggggtggcc
     3181 gtagaggtga accggtatga tggcacgcgt acgtggagtg atctttgctt cgaccagctc
     3241 ggcggtcata ttgaacgtct tggggtccac atccacgaaa accggcgtgg caccagtata
     3301 cgagacagca aaggcagtcg ccacatacgt gttgctgacc gtgatcacct cgtcaccagg
     3361 gccgatatcg agagcctcta aggccaggtg caatgcagct gtaccactgg atattccgat
     3421 gctgtacttg caatcgcaat aagccgcgaa gtccttctca aactgagcca gatgggggcc
     3481 aagagtatat tttccagtgg gtagcaccgc atcgactgcg gcgcgcacct cgtcagcaat
     3541 ctcttgatat tggcgttcca gatggccttc gccgacctcg actggacggc gggcgatttg
     3601 cagccactcc gggatttcga ccgggagcgt gagatcaatt tttgtcattc ttatctccat
     3661 agaactgcgg caggtaatct cgattagctt cgagcatctc gtccaccagg gccgcggcga
     3721 cctcgtaaga cgggacaagc gggttcagca acatcgccag gcgggcatcg cgacgagagc
     3781 cagaaacccc agctttcacc gtggccgact cccaggcctt ggcctgctgg caaagcgagc
     3841 ggatcgtctc tggcagcggt cccatgctca gcggagtgac cccgtggctg cccaccacgc
     3901 atggcacctc tacggaggca tccggcggca ggtcgggcaa gctggttccg ttgcgggtga
     3961 ccacgatctg aatgtcgcga cggtcaagca tgagcgatag gatcaaattg atcgctgcct
     4021 ccgaatagcg cgcgccaccc cgcttggaga gctcaggtgg cactaccacc tggttcgggt
     4081 cggcgtactt tttcatcagc gcctcttcga cctcgcgcac gacttcgccg cgcgtttttt
     4141 cactggtgcg cgcctccgac agcttgcggg gatggttgta atagtattcc aggtaataag
     4201 tcgggaggac gcccagggta cgcaccagct cagggtcaaa gtgcggcaga tcctccaatt
     4261 gactcaagcc ttccagacct gccataatat ctgaggtgac atccttaccg cgcacatata
     4321 cacgccgcac ccaattgacg tgattcagtc caacccactc cagaaagaca tcttgcggtt
     4381 ccactccgaa aaattccgcc atgccagctt gcgtgttaat ggggttgtta cacaggccta
     4441 caaccttgat gtcggtgtgt tttaagatgg cttcggtgat gatgccagaa gggttggtga
     4501 agtttaaata ccacgattcg ggagcataac gcgccatgtc acgggcgacg tccagggtaa
     4561 cagggatcgt ccgccaggcc ttcagggtac caccggggcc ggtggtctcc tggccgataa
     4621 ccccgtattt aagggggatg cgctcgtcac ggatgcgggc tggcatctgc cctacacgca
     4681 tggaggagag gacaaattgg gcgccctcaa cggaagcagg gcggtcgtgg ctgagcgtga
     4741 tcttggtctc aagctcagca tactgcgcca tacgctgcgc catgccaccc acgattttca
     4801 aacgctcctg gtcaacatcg aaaagaacga tctcgccgac gcgcaccttg tcttcgtttt
     4861 gaatgaagcc atcaatcagc tcgggagtat aggagctccc gccgccaatg acggtcagct
     4921 tttcgatgct tggttgcgcc attgcgcgac ctccgcttcc agcgcatcca tacgctgata
     4981 catcttgata aattgttcgg tgtaatccag ctcactccag gctagtagca acaagtccat
     5041 ggcatgcacc agcaggactg agaaagggac ggcctgatcg ctggcttcca ggcgtatgat
     5101 gtctgcatta atttgatgcg ccttgagttg ctcctcgttg gcttcatcca gcgcagcttg
     5161 agcttcatcc cattcgccag tccgggcgtg ctctgcagcc tctttggctt ttgagcgggc
     5221 attgccggca cagagaatca ggttaaaaag ggtctgctcc cattcgagtt cgtccgtcat
     5281 atcgacctct cttcttgaag atgcacgtgc tcgttcatgc ggatagagtg ccacacgcca
     5341 tcctcggcgt gcaattctga gatactgcag gcctcccatg ggccatagtc tcgccagcgc
     5401 tgcaaatccc cgtccaacaa tagcgccatc agcatgctga ttacgcccca atgggtcaca
     5461 accagcaggc gaccgttggg atactgcgcc tgcatagccg ctattgcctc ccgaatacgc
     5521 gctgcaaagt gctgagttcg ctcagcggtt aaacgcatcg caaatcgcac gagcgggcgg
     5581 aacagccaga atccggtgcc atccgggtca aagtgcgtga gtggcttgcc ctcgctccag
     5641 ccgaaatcca tttcgctcaa tccatcgagc gcgatgggct ccatcccaag tgcttctccc
     5701 aggatgaggg cggtctgcat ggcacggcct ttcgggctgg caaacaaagc gtcaaaggtt
     5761 tccccacgca aacgatctgc ggtttgtttg gcatgctgcc taccggtggg gctcagcggg
     5821 ctatccaggc gaccatgcaa taggccaagc tcagtccact ccgtttcgcc gtggcgcaca
     5881 aggatgagtt gtagtgtcgg ctgcattata aagctacgcg cccccgcttg cagcggtctc
     5941 catgggcagc gcgttgtcgg cggcacaacg aaaacccata aagtcgttct tgcgcttagg
     6001 ggtgtgcata cggcggaaga aggtctgagc ctcatcaggt ccatccaacc aatccccacc
     6061 gcgcaacggc atgcgcaggg gcgagggttt tccgccgatc cactcccaaa cattgcccgc
     6121 cgtgtcccat aagccatatg ggctgtcgcc gcccggcgag tacgctccta cgggtgtggt
     6181 accttctgag ctggtattgc attttctggg atcgaagacg ttgccccacg gccaaacacg
     6241 cccatcagtg ctacgcgcgg ctttctccca ttccagttcg gtgggcaatc gtttcccggc
     6301 ccattgggta tatgcaaagg cactatccca ggtgatctgc accaccgggt ggttcgcctt
//
LOCUS       DBNGSX010000102         9593 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1323350, whole genome shotgun sequence.
ACCESSION   DBNGSX010000102 DBNGSX010000000
VERSION     DBNGSX010000102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9593)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9593)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9593
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1323350"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="AC16CB_03510"
     CDS             <1..581
                     /locus_tag="AC16CB_03510"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039905.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR3341568.1"
                     /translation="VIAAGKSRTIDLGRVTFGAEKTCRYFSNNSAIGMEPTVTLIQEE
                     ELVRMKGIARYLAATIIAVFRNAHWTVSLEWEGGDYQGPANLVTVGNSPLTGGLFYMT
                     PHANPEDGLLTCVFGYLPSRLKILSVLPKTMKPGEGSYVEHPAIQEIHTPWLKIHTHT
                     PTPLHTDGVIQSKSVTDVEYSILPSKLSILIP"
     gene            636..1247
                     /locus_tag="AC16CB_03515"
     CDS             636..1247
                     /locus_tag="AC16CB_03515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3341569.1"
                     /translation="MRVFFKLLYHQFACGYDLVSTIVSAGRWRMWVVSVLPDVNGPLV
                     LEIGHGPGHLLTELGDRGTRVVGLDESWPMSRLAFLRLVSLGLSRLLINGYAQFLPFR
                     NNTFNTVVSTFPSEYILDTRSLSEVLRVLVPGGKLIILPIAWITGNGLYDRLAAWLFR
                     VTGQTPDWDELYTQHLVSTGFSVSIEHRALISSQLLIVHAEKP"
     gene            1298..2005
                     /locus_tag="AC16CB_03520"
     CDS             1298..2005
                     /locus_tag="AC16CB_03520"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR3341570.1"
                     /translation="MDIRIAVAKTRKYATSDSGDTVEIIERPNGGLSVVMADGQSSGR
                     GAKSISTMVVRKVISLLAEGVRDGAAARAASDYLFTERGGKVSATLNILSVDKQTRTL
                     VITRNNPAPVLRAIGDEIRSLDEKCVPIGLYRGTRPLISEVPLEVGLTSVMYTDGFIH
                     AGSRYGESMDVVACLQGLLEDENPSPQLIADDLLDTAIRLDHGRPSDDISIVVLRVTR
                     NAGDNVRRMYVRLPFDS"
     gene            2017..2382
                     /locus_tag="AC16CB_03525"
     CDS             2017..2382
                     /locus_tag="AC16CB_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HIR3341571.1"
                     /translation="MKSLIIGVVGPCGAGKTTLIQGLRANGYLAKHIAQEHSYVPQMW
                     SRLTNPDILIYLDVSYPLSIQRRRIDWTSSEYSKQLRRLKHARENADFYLNTDDLTPQ
                     EILQQVAEFIDRYQIGHSG"
     gene            2476..3843
                     /gene="secD"
                     /locus_tag="AC16CB_03530"
     CDS             2476..3843
                     /gene="secD"
                     /locus_tag="AC16CB_03530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HIR3341572.1"
                     /translation="MQRNQIARIVLIVVLIIIAIYLDIPNTPGIPGSERKIETRLGLD
                     LVGGVQALLEADLPSEQAIDTEAMQTARSIVENRVNGLGVTEAVVQQAGDRRIVVELP
                     GEKDPEQALATLKQTGLLEFVDFSELTPQEAFTLVGTKIETDFATQQSSVQSSETLTP
                     TQAAKIFPTVMTGSALKSVSVTSTQLNVEPQVAFELTSEGAQIFKDYTTANVGQVLAI
                     VLDKEVISVPTINEPITEGQGTISGNFTIESANVLAVQLRYGSLPIPLKIVESRTVGP
                     TLGQDSLRKSLVAGAVGFAVVILFMGLYYRLPGFVAIFSILVYALITFALFRTIPITL
                     TLPGIAGFLLSTGSALDANILTFERLKEELRAGQTLRQAVKLGWDRAWPSIRDSNIAT
                     LITCAILYYFGSTLGATIVKGFSLTLFLGVLVSLFTAIYVTRTTLTLVLEYIKPTNLA
                     RWFGI"
     gene            3857..4780
                     /gene="secF"
                     /locus_tag="AC16CB_03535"
     CDS             3857..4780
                     /gene="secF"
                     /locus_tag="AC16CB_03535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HIR3341573.1"
                     /translation="MINLLGKRYYFFLLSLLIIIPGMVVLAVKKFPLSIDFTGGTLLE
                     VQFEKQTPQPAEVIAVYQDLGVEDVLAQTTSEGTIVARSSFLDDNVRSKIVDELSKEL
                     NQKVTILKLDSVGPSIGQEVAQKAAIAVGVAALIVVLYMTFAFRGVEHALRYGICAII
                     AMVHDVAIVVSLGAIGGHFWGWQIDSLYLTAVLTVIGFSVQDKIVVFDRVRENSRVFR
                     RLDFETLVNHSIIQTLQRSINTQLMTVEFMLLALALFGGVTLREFAVILLVGLFSGTY
                     SSIFIAAPILVVWENREWQHWFTRKDRQAIA"
     gene            complement(4855..5625)
                     /locus_tag="AC16CB_03540"
     CDS             complement(4855..5625)
                     /locus_tag="AC16CB_03540"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIR3341574.1"
                     /translation="MPTILAIILLGLVAGAIVNYLADVLPIKRQLVAPICLNCESRQN
                     LVNYLVLPRRCEVCGQPRNIRSYVVDVVFALSAVWLWQSPPEDIGFVSGMLLLTFFGV
                     VTVIDIEHRLILNPVSLVGAIIGLSVGTWLHGLKSTLIGGVAGFLAMLLLYLLGGLLM
                     GWLARRRGQILDEDALGFGDVNLSGILGLLLGWPGILIGLILTILLAGIVSFVYLLGA
                     LLTRSYRMNMALPYGPFLISSAVALLFFREYVLQYMGW"
     gene            5753..7045
                     /gene="murA"
                     /locus_tag="AC16CB_03545"
     CDS             5753..7045
                     /gene="murA"
                     /locus_tag="AC16CB_03545"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR3341575.1"
                     /translation="MDKFIIQGGVPLTGQVTPSGNKNAALPLLAACTLTEEPVILHNI
                     PQIRDVNTMCLLLESLGVQIDKIDDNSWRIQAKHVRPADLDPNLCKQIRASILLAGPM
                     VARSGLLELPPPGGDIIGRRRVDTHILALKALGAQADFHRDEHIFRFSAKKLTGANIL
                     MDEASVTATENAIMAAVTASGTTVIRNAASEPHTQELCSFLNVMGAKIENIGSNTLEI
                     HGVPSLHGGEYTLGPDYLEVVSFIGAAAITKGSIRIHKAAPEYLDMIAIVFRRLGVSW
                     EVEGEDIVVPAQQSMEVEPDLGGVIPEISVMPWPSFPTDLMSIAIVIATQSKGSVLFH
                     DWMYPSRMFFIDKLVGMGAQIILCDPHRCIVQGPTQLYGENLESPDIRAGMALLLAAL
                     ASKGKSVIRNVGQIDRGYERVDEKLYALGAHIKRVHQD"
     gene            complement(7107..7406)
                     /gene="trxA"
                     /locus_tag="AC16CB_03550"
     CDS             complement(7107..7406)
                     /gene="trxA"
                     /locus_tag="AC16CB_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR3341576.1"
                     /translation="MQSTIPVVVDFWAPWCGPCKMIAPTLEKLAKEHAGKLLIAKVNT
                     DEHPEWSMRFGVQGIPTMLFIAGGKVIHRQVGALPEPMLRDIIGQFLEVVQKVES"
     gene            7647..8345
                     /locus_tag="AC16CB_03555"
     CDS             7647..8345
                     /locus_tag="AC16CB_03555"
                     /inference="COORDINATES: protein motif:HMM:NF014654.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR3341577.1"
                     /translation="MHLIERAELDITRLALAQVTDQFLAYINNMPELSADEVSAFLVI
                     AAKLIQIKSEALLPRPPVREEGEEDPGEALARQLLEYRRYKYIADFLSQKEELGLQTY
                     LRLAPPPRIEGVVDLSEIHLDDLVAAAQAILHKIDPRSSLNSVVSPSPVTIRQKISRI
                     ARILRRERRVSFFTLLNGARTRLDIVVTFLAILELIKQKIVLASQTMQFGNIEIEALK
                     EWDEDVEFELEFDD"
     gene            8387..>9593
                     /locus_tag="AC16CB_03560"
     CDS             8387..>9593
                     /locus_tag="AC16CB_03560"
                     /inference="COORDINATES: protein motif:HMM:NF015168.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR3341578.1"
                     /translation="MSNLLANENSPYLLQHANNPVQWYPWGNEALKKAQREEKPIFLS
                     IGYAACHWCHVMAHESFEDPDTASIMNQRFINIKVDREERPDLDNIYMNAVISMIGHG
                     GWPLSVFLTPEGHPFFGGTYFPPTRRYNMPSFREVLLSVYNAWTEDRAHILDVAQQIS
                     SRLAQSTIKSGEYPEVTPETLDNASLALAQTYDWKYGGWGSAPKFPQPMTIEFLFRRA
                     STGDKLALDLATHALRAMAKGGMYDVIGGGFARYSTDNEWRVPHFEKMLYDNALLSQV
                     YLHAYLLTGDKSFRRVCEETLDFIARELCHPSGGFFSSLDADSEGEEGKYYLWTTATI
                     QAAISDKEQLEMVTAAYEITDSGNFEGSTVLQRVIDDASLAVRFAIAEEDVPLRLAEI
                     HKQLLRAREK"
BASE COUNT         2555 a         2283 c         2250 g         2505 t
ORIGIN      
        1 aggtgattgc ggcaggtaag agtcgaacga tcgacttggg acgagtcacc tttggggctg
       61 aaaaaacatg tcgttatttt agtaataact ctgcaattgg catggaaccg actgtcacac
      121 tcattcaaga agaagagctc gttcgaatga aaggtatagc ccgctatctg gctgctacga
      181 taatagccgt ttttcgaaat gctcattgga cggtttcgtt agaatgggag ggcggggatt
      241 accagggtcc ggctaatttg gttaccgttg ggaatagtcc gctcacggga ggattattct
      301 atatgacccc tcacgctaat ccggaagatg gcttattaac ttgtgtgttt ggatatctcc
      361 cctcgcgtct aaaaatattg agtgttttgc ctaaaaccat gaaaccgggc gagggaagct
      421 atgtggaaca tcctgctata caagaaatcc acacgccttg gttgaaaatc catacacata
      481 caccaacccc cctgcatact gatggcgtaa ttcagtcgaa gtctgttact gatgtagagt
      541 acagtattct tcccagcaaa ctttctatct tgataccata aataccctca ttagcgctga
      601 tgaactcgct cccctaccgc atccttatgt cctttatgag ggtgtttttt aagttgctct
      661 atcaccaatt tgcctgtgga tatgatttgg tatcaactat cgtctctgct ggcagatgga
      721 ggatgtgggt agtctccgtc ctccctgacg tgaatggccc attggtattg gaaattggtc
      781 atggtccggg tcatttgctg acggaattag gagatagagg tacgagggta gttggcctgg
      841 atgagagctg gccgatgagt cgtttggctt ttttgaggtt ggttagtctg gggttaagcc
      901 gattgttaat taatggctat gcgcaattct tgcctttccg caacaacacc tttaatactg
      961 ttgtctccac tttcccttcg gaatacatac tagatacccg atcgctctcg gaggtcttgc
     1021 gagttctggt accgggtggc aaattgatca tcctccccat cgcatggatt actggcaacg
     1081 gcttgtacga tcgcctagct gcgtggctct ttcgcgtcac tgggcaaaca cctgattggg
     1141 acgaactcta cacccagcat ctcgtctcta ccgggttttc tgtaagtatc gagcaccgcg
     1201 cattgatatc aagccaacta ttaatagttc acgcggaaaa accttagttc aacgtagtct
     1261 aggcctaaaa atctctatac tgatgtaaaa ttgggttatg gatatcagga ttgccgttgc
     1321 caaaacccgt aagtacgcca cttccgatag cggtgacaca gttgagatca ttgaacgccc
     1381 caacggtggt ctctcggtag tcatggccga tggccaaagt tccggccggg gcgctaagtc
     1441 gatttcaacg atggttgtcc gaaaggtgat ttcccttttg gcggaaggcg tgcgcgatgg
     1501 agccgcagct cgggcggcgt ccgattattt atttacagaa agaggcggca aagtttccgc
     1561 aacattaaac atcctctccg tagataagca aaccagaacg ttagtaatta cacgcaacaa
     1621 tccagctccg gtattgcggg ctattggcga tgagattagg tcgctcgatg aaaaatgcgt
     1681 acccataggc ctttatcgag ggactcgccc actaatcagc gaagtgcctc tagaagtagg
     1741 tttaacttcc gttatgtaca cagatggatt catccatgcg ggatctcgct acggcgagag
     1801 catggacgtt gtcgcctgtt tacagggatt gttggaagat gagaacccgt ctcctcaact
     1861 gattgcagat gatcttttgg atactgctat acggcttgac catgggcgcc cgtcagacga
     1921 cattagcatt gtggtgttac gtgtgacccg caacgctgga gacaatgtcc ggcgcatgta
     1981 tgtacgcctc ccatttgata gttaagatct gttgccatga aatccttgat aattggtgtt
     2041 gtcggccctt gcggggctgg caagacgacc ctgatccagg ggcttagagc gaatgggtac
     2101 ctggcgaagc atattgcaca ggaacactcc tacgtacccc aaatgtggag taggctgacc
     2161 aatccagata ttttgatcta tctggatgtt tcttacccgc tatcaattca gcgccgaaga
     2221 atagattgga cttcatcaga atattctaaa cagctccggc gtctaaaaca cgcccgagag
     2281 aatgccgatt tttacctgaa tacagatgac cttacccccc aagagatatt gcaacaagta
     2341 gcagagttta ttgatcgata tcaaattgga cattccggct aattcggagt ataatgcgac
     2401 cctgccagcc caaagtgtga ggtcgatcag tcaaaggctg gcatttgttt gaattatgga
     2461 ggcggaagaa gagtaatgca acgtaatcaa atcgcgcgaa ttgtcttgat cgttgttttg
     2521 atcatcattg ctatctattt agatatccca aatacccctg ggattcctgg tagtgaacgc
     2581 aaaatagaaa ctcgcttggg tttggacttg gttggcggag tccaggcact tttagaagct
     2641 gatttaccct ctgagcaagc catcgatacg gaggccatgc aaacagcccg ctcgatcgtt
     2701 gagaaccggg tgaatggtct tggggtgacc gaagctgttg tgcagcaggc tggagatcgg
     2761 cgcattgttg tggaattgcc cggagaaaag gatccagagc aagcactcgc caccctaaaa
     2821 caaactggct tgctggagtt tgtggatttc agtgagctca ctccccagga agctttcaca
     2881 ctagtaggta caaagatcga gactgatttc gcaacccagc agtcatctgt ccagtcctca
     2941 gagacactga cccctaccca agcagcaaaa atcttcccaa cggtgatgac cggcagcgca
     3001 ctaaaatctg tatccgtcac aagtacacag ctaaacgttg aacctcaagt cgcgttcgaa
     3061 ctaacgagtg aaggcgcgca aattttcaaa gattacacaa cggccaatgt cggacaggtg
     3121 ctggctatcg tgctagacaa agaggtgata tcagtcccca cgatcaatga gccaatcact
     3181 gaaggccagg gcacaatcag cggcaatttt actatcgaaa gcgctaatgt attagctgtt
     3241 cagttgcgtt acggctcttt gcctatcccg cttaaaatcg tagagagtcg cacggtaggc
     3301 ccaactttgg gtcaggattc actacggaaa agccttgttg caggggcagt tggctttgca
     3361 gtcgtgatat tgtttatggg actgtattat cgcctgccag ggtttgttgc tatcttctcg
     3421 atcctggtgt atgcccttat aacctttgcg ttgtttcgaa ccatacccat cacccttact
     3481 ttacctggta tcgccggctt tctattaagc actggcagcg cactggatgc aaatattctt
     3541 acattcgaac gattaaaaga agagctgcgc gctggtcaaa ctttgcgcca ggcagtcaaa
     3601 ctcggatggg acagagcctg gccttccatt cgcgattcga atatcgcaac attgatcacc
     3661 tgcgctattc tttactattt tgggagcact ttaggggcaa cgatcgtcaa gggcttttcg
     3721 ctcaccctgt ttctaggcgt gcttgtcagc ttattcaccg ctatctacgt cactaggaca
     3781 actttaactc tcgtattgga atatatcaaa ccgaccaatc tcgctcgctg gtttggtatc
     3841 taaaaggatc gcagtcatga tcaatctcct tggcaaacgc tattacttct ttctgctctc
     3901 gctgctaata atcattcccg gcatggttgt cttggcggtt aagaaattcc ccctctcgat
     3961 cgatttcact ggtggaactc ttttagaagt gcaatttgaa aaacaaaccc ctcaaccggc
     4021 tgaggtcatt gctgtttacc aagatcttgg tgttgaagac gtgctggcac aaaccacaag
     4081 cgagggcacc atcgttgcac gatctagctt tttagatgac aacgtgcgca gtaagatcgt
     4141 tgatgagttg tctaaagagc tcaaccaaaa agtgaccatt ctgaaactag acagcgtagg
     4201 cccctcgatt ggtcaggaag ttgcccaaaa agccgccatc gcggtcgggg tcgctgcgtt
     4261 aattgtagtc ctatatatga cctttgcatt tcgtggagtg gagcatgcct tgcgttatgg
     4321 gatttgtgcc attatcgcca tggttcacga tgtggctatt gtagtgagcc tgggcgccat
     4381 cggtggccac ttctggggct ggcaaattga ctcgctatat ttgacagcag ttttgactgt
     4441 gatcggcttt tctgtacagg ataaaatagt ggtttttgac cgggtgcggg agaatagtcg
     4501 cgtttttcgc cgattggatt ttgaaaccct ggtcaatcac tccatcatac agacgctaca
     4561 acgctctatc aatacccagt taatgacggt agagtttatg ttgctcgccc tggctttatt
     4621 tgggggcgtc acattacgtg agtttgcggt aatactgctt gtaggccttt ttagcggtac
     4681 ttattcatcg atattcattg ctgcgcctat cttggttgta tgggagaatc gtgagtggca
     4741 gcactggttt acacgcaagg atcgccaagc catcgcataa taaaaacagg tcaaaaaagg
     4801 ccgggtgtag agttacaaac cggcctttta tttggatatc aaaggttgtt gtggttacca
     4861 gcccatatac tggagtacgt attctctgaa aaatagcaag gcaaccgcac tgctgattaa
     4921 gaatggccca taaggcaggg ccatattcat ccggtagctg cgagtcagca aggctcctaa
     4981 caaataaaca aagctaacta ttcctgcgag caggatggtt agaatcaaac cgattaggat
     5041 tcctggccat cctaaaagca atcccaatat accgctcaaa ttgacatcac caaatcccaa
     5101 agcatcctca tctaatatct ggcctcgcct gcgagccaac caacccatta atagtccacc
     5161 cagtagatac aacaaaagca tagccaaaaa tcctgcaacg cccccaatta gtgtagattt
     5221 taagccatga agccaggtgc ctacagacag tccaattatc gcgccgacta agctcactgg
     5281 atttaaaatt aacctatgtt ctatatcaat cacagtgacc accccaaaaa atgtcaataa
     5341 aagcatcccg cttacaaatc cgatgtcttc tggaggcgat tgccatagcc ataccgccga
     5401 gagagcaaaa accacatcaa caacatatga ccgtatattt cgaggctgcc cgcacacctc
     5461 gcacctgcga ggcaaaacca gataattgac caaattctgg cgggattcgc agttcaaaca
     5521 gattggcgca acgagttggc gttttatcgg caaaacatca gccagataat ttacaatagc
     5581 acctgctacc agacccagaa gaataatagc taaaattgtg ggcataaatt agacctaatt
     5641 atttcatcct ttgaaaatta gtataatggg cgtattatag cagggtctct ggccagagta
     5701 tagacacagc cagacgcatc cttctgaaaa tcaatgattg ccaggagatt caatggataa
     5761 attcatcatc cagggaggtg ttcctctgac tgggcaagta accccgtcgg gtaataaaaa
     5821 cgctgcacta cctctccttg cggcttgcac actcaccgaa gaacccgtca tattgcacaa
     5881 tatcccgcaa attcgcgatg taaataccat gtgcttacta ttggagagcc tgggtgtcca
     5941 aattgacaaa atcgatgaca atagctggag aattcaggca aagcatgtgc gtccagccga
     6001 cttagatccc aacctgtgta agcagattcg agcatctatt ctgctggcag gaccgatggt
     6061 tgctcgatcg ggattgttgg aacttcctcc accaggaggc gatattattg gcaggagacg
     6121 tgtagatacc catatccttg ccctaaaagc gcttggggca caggctgatt ttcatcggga
     6181 tgaacatatt ttcaggttct cagccaagaa actgaccggc gcaaatatat tgatggacga
     6241 ggcaagcgta actgcaactg aaaatgcaat tatggccgct gtaacagctt cgggaacaac
     6301 ggttattcgc aatgctgctt ccgagcctca cacccaagag ttatgcagct ttctcaatgt
     6361 aatgggggct aaaatagaaa atattggctc aaacacgctc gaaattcatg gtgtgccaag
     6421 cttgcatggg ggtgagtaca ctctcggacc cgattatctt gaagtggtca gttttattgg
     6481 agccgctgcc ataactaagg gatcaattcg tattcacaaa gcggctcctg aatacttgga
     6541 tatgatcgcc attgttttcc gcaggcttgg agtgagctgg gaggtggaag gtgaggatat
     6601 cgtcgttccc gcccagcaga gcatggaggt tgaacccgat ttaggaggcg taatacccga
     6661 aatcagcgtg atgccctggc cttctttccc caccgacctg atgagcatcg ctattgtcat
     6721 tgccacacaa tccaagggta gtgttttgtt ccatgactgg atgtacccca gccgaatgtt
     6781 tttcatcgac aaattggtcg gcatgggcgc ccaaatcatc ctatgtgatc cccatagatg
     6841 tattgtccaa ggtcccactc agctttacgg agaaaatttg gaaagcccag atatacgcgc
     6901 agggatggct cttttgcttg ctgccttagc gagcaaaggg aaatctgtca ttcgcaatgt
     6961 cggtcaaatt gatcgcggat acgaacgagt ggatgaaaaa ctatatgcgt tgggtgcaca
     7021 tattaaacga gtccatcagg attgagaaat aaactacgcc cagcactttg cctgctgggc
     7081 gtagcaaaac caatccgtta aagggcttaa gattctacct tttgtactac ttctaaaaac
     7141 tgtccaatga tatcacgcag catcggctca ggtagcgctc ccacttgccg atgaattacc
     7201 ttcccacccg ctataaacag cattgttgga atgccttgca cgccaaacct cattgaccac
     7261 tcaggatgct cgtcggtatt aactttagct atcaatagtt tgccagcatg ttccttggcg
     7321 agtttctcta gtgttggggc aatcattttg cacggcccac accagggagc ccaaaaatcc
     7381 acaacaactg gaatggtgga ttgcaatact gtcttctcaa agcagcatca gtaacatgga
     7441 taggttcttg aaccatcaat ttacctccta ataatcaaat ctttgcatta taaagattag
     7501 atgcagattt cctctaaagg ttacaaacaa agtatatcat ttaattgtca ggtataatcc
     7561 aagccatgta tcccgaacgg gtaagtccaa agccacagcc atataccgtt tcaacgccag
     7621 tgtatcaagg gccattggat ttattgttgc accttattga acgtgcagag ttagatatca
     7681 ccagacttgc tctcgctcag gttacggatc agttcctcgc atatatcaac aatatgcctg
     7741 agctctctgc cgacgaggtt tctgctttcc tggtcattgc agcaaagttg atccagatca
     7801 agtccgaagc tttgttgccg cgcccaccgg tacgtgaaga aggcgaagag gatccaggcg
     7861 aagctctggc gcgccagtta ttggaatacc gccgctataa atacattgca gattttttat
     7921 cacaaaaaga agaactggga ctgcagacgt acttacgcct cgctcctccc cctcgcattg
     7981 aaggagttgt cgatttgagc gagatacacc tggatgacct ggtagctgcc gcccaagcca
     8041 ttctgcacaa aatagacccg cgttcatcat taaattctgt tgtcagtcca tctccggtga
     8101 ccattcgcca aaaaatcagt cgcattgccc gaattctacg ccgagagcga agggtatcct
     8161 tttttacact gttaaatggc gcgcgcactc gtctggatat cgttgtgaca tttcttgcaa
     8221 ttttagagct catcaaacaa aaaattgtcc ttgccagtca aacaatgcaa tttggcaaca
     8281 tcgaaattga ggcacttaaa gaatgggacg aggacgtgga gttcgagtta gaatttgacg
     8341 attagccatt tcgcttaccc ctacctgaac ctgattgata attcacatgt ctaatttatt
     8401 agcaaatgaa aattccccat acctgctcca acacgcaaac aatccagtcc aatggtatcc
     8461 atggggcaac gaagcattga aaaaagctca aagggaagaa aaaccaatat tcctgagtat
     8521 cggctatgct gcctgccact ggtgccatgt tatggctcac gagtcatttg aagatcctga
     8581 tacagcatcc atcatgaacc agcgattcat aaatatcaag gtggacagag aagagcgacc
     8641 tgatctagat aatatatata tgaatgctgt tatatccatg attggacatg gaggctggcc
     8701 tttgagtgtt tttctcaccc cggagggaca tcctttcttt ggtgggacct attttccacc
     8761 aacccgtcga tataatatgc cctcatttcg cgaggtcctg ctgagcgttt ataacgcatg
     8821 gacagaagac cgggcgcata ttttggatgt tgcccagcaa ataagcagtc ggcttgcgca
     8881 atctactatc aaaagtggtg aatacccaga ggttaccccc gagacgctgg ataatgcatc
     8941 ccttgcttta gcacaaacct atgactggaa atacggtggt tggggtagcg cgccaaaatt
     9001 cccgcaaccc atgaccattg aatttttatt tcgccgagcc agtacgggtg acaagcttgc
     9061 tctcgaccta gctacgcatg ctttgagagc catggctaaa ggcggcatgt acgatgtgat
     9121 tggaggaggt tttgcccgct atagtaccga taacgagtgg cgcgtcccac attttgaaaa
     9181 aatgctctat gataacgccc tactatccca ggtatatctc catgcttacc tacttactgg
     9241 agacaagagc tttcgtcgcg tttgcgaaga gactttagat ttcatagctc gcgagctttg
     9301 ccacccatct ggggggtttt tcagcagcct agatgccgat tcagaaggag aagaaggaaa
     9361 atactatctt tggacgactg ccacaattca ggccgcaatc tccgacaagg agcaattgga
     9421 gatggtcaca gccgcgtacg agataacaga tagcggaaat ttcgaaggaa gcactgtatt
     9481 gcagcgggta atagatgatg catcccttgc agtaaggttt gccatcgccg aagaagatgt
     9541 tccactccgc ttagcggaaa ttcataagca attattgcgc gcgcgcgaaa aac
//
LOCUS       DBNGSX010000103         9016 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1254098, whole genome shotgun sequence.
ACCESSION   DBNGSX010000103 DBNGSX010000000
VERSION     DBNGSX010000103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9016)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9016)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9016
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1254098"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2925)
                     /locus_tag="AC16CB_03565"
     CDS             complement(<1..2925)
                     /locus_tag="AC16CB_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like domain-containing protein"
                     /protein_id="HIR3341579.1"
                     /translation="MINLFNLTRNQRLFIKGQSFILLFAFLFGVIAPAFQTITAYAAG
                     GDFANTDFAASGPYSYDHAIGGGAYDDGSNSKATGLVESLEGSEFACGDVVTFLTAIK
                     ISAKPVHANQVAEFDYKFLADSTGQSGAAITDIIDVRVNRGVVSNGAGAGGTDAGLID
                     DGGSVATLVSETVVGTLFTPGSEVHGTVRVTDLEANEQVILRIDVRLSCQPGTSPTGN
                     LQGQLDAGRVVSPVSDTINTGQQTIPFLKIGDLLGAGEPLLSISKTVTTADGICPGSE
                     TLTVNAGDTVKYCYTVSNPGTAELYNVTLVDDNGTPANSVDDFSVSLSGLTSGGLLAG
                     DSATGSALVTLSSAGTITNIANANGNNNQSGQKLVSYNDQDSATVFVMGPANSAPAAV
                     DDSTTVPEDGSVTIPVTGNDSDADGNLDPSTATVIDAPMYGTLVNNGDGTFTYTPNPD
                     YNGSDSFSYQVCDVYGLCDTALVNITVTSANDVPVATDDTASTLEDTSLVINIVDLLL
                     NDLDADGDSLTITGIASQPQHGTLSDNGDGTYTYAPAQGFFGEDSFTYTVCDPSGACD
                     TATVIITITPVNDAPEASDDSASTEEDTAVNINVLGNDSDPENNLDAGSVTIVEPPAN
                     GSLAVNPDGSVTFIPNTNWSGTDSFTYQVCDSADPALCDTATAIITVIPVNDAPVAND
                     DAATTLEDQAVVIAVLPNDTDADGDALSISSYTQPANGSVVLNADGTFTYTPNSNWYG
                     TDTFNYTVCDLSGACDEAMVTVEVLPINDPPVAEPDAATTAEETSVKVDVAMNDFDVD
                     GNLVPSSALQISEPANGSLINNGDGTFTYTPNENFNGYDSFRYQICDAENECSTAEVT
                     IYVTPVNDAPVALDDATTATEDTPLVINPIDLLGNDSDVDGDSLNISGFTQPSNGTLT
                     YENGTFTYSPNANFCGSDSFTYTISDGELSAIAAVTIEVVCVNDAPVALDDATIATED
                     TPQ"
     gene            3258..3938
                     /locus_tag="AC16CB_03570"
     CDS             3258..3938
                     /locus_tag="AC16CB_03570"
                     /inference="COORDINATES: protein motif:HMM:TIGR04305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CADD family putative folate metabolism protein"
                     /protein_id="HIR3341580.1"
                     /translation="MNPQETLSQLDQLIHSRSILCHPFYVAWQRGELTRDQLATYAEV
                     YYPHVASFPGYLKAAIDAADDDGVRQELERNLADEIANPKPHPELWLDFAAGLGLDPA
                     NIANGAARPAAIQMVNTFDRLAQGDSAAALAALYAYESQQPEVSRQKIEGLRLRYGVD
                     SLDALAYFEVHAEADIQHRDGERQALLRCLKNGVSAQIVLESAEQALDAYWGLLDGIC
                     EEAGITTG"
     gene            complement(4046..7186)
                     /locus_tag="AC16CB_03575"
     CDS             complement(4046..7186)
                     /locus_tag="AC16CB_03575"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3341581.1"
                     /translation="MNERQKLEQAIAALEAQRNVLGDAVVDPAIAGLRKQLAELEPPS
                     EQQLKLATLLYTDIVDSTHLIEGLDPEDIIHIMDGALKYLSTVIDQHGGHVTRFMGDG
                     FKAVFGTPVAREDDAEQAVRAGLGLLKAAEVYAAKIEQEWGRSGFNIRVGINTGPVAI
                     GGLSEAGDTIMGLTVNVAARLENAAPAGGVLISHNTYQHIRGVFDVQALEPLHAKGIA
                     EPILVYLVQRIKARAFYRESRGVEGIETRMVGRDAELRHLQDALYSAIEKRQVHRVTV
                     IGEAGLGKTRLLYEFENWFDLLPEEFWVFKGRAFRYTQSLPFALARDIFSLRFQIQDS
                     DSSAVAKQKLEAGFVDFMGEAGIEKSHFIGQLLGFDFTDSPFIAGIRGDVKQIRNRAF
                     YYTTQFFSAVAAQDPVIILLEDIHWADDGSLDLVEHLSRNWRGFPLLIVGLTQPSLFE
                     QRSTWGEGDDNHSRIELHPLSKQECRRLVLEILRKVVDLPQVLEKTIIGTADGIPFYV
                     EELVRMMIEDGVIIKGEQEWRIQPDKLAKLRVPPTLIAVLQARLDRLSQTEREVLQRA
                     SVVGRIFWETTLEQMFENNQAGSITNVSELHQILQILRSREMIFRHEPSAFDGTVEFI
                     FAHTMLHEVTYESVLKRDRRAYHAQVAAWLEKQGSERVDEHAGLIAGHYALAGVANKA
                     VRWYIRAADLALRISAFGEARAMYTQALDLLSGKDQDRLPIQLHLGKVYYQLSDYSAA
                     RETLEIAQRIAQDLDDRPRSADALYWLSQVASAGGDYQQAQAYLEESLPLARVAGDRA
                     TLAQVLYGLGDLQWRMGNFDQARLNCEESLVLARQLGDLNLELYALNRLGTVPLVKGE
                     LDKAQPFFEETLERARQAGNRDREANALNNLGAVAYERGDLVATQAYFQNALSLVREI
                     GQQEFEAVLLSNLADISIRLDKLPAARKYICEALDIALAIGAIPGVLFSIKNIGWLLT
                     KQGQTERGLALLGMVLYHPAAFSENIREVHNALEHLGLVADEPVVAAGLELGKNLALD
                     LVAAELLMEMRCAKEY"
     gene            complement(7228..7710)
                     /locus_tag="AC16CB_03580"
     CDS             complement(7228..7710)
                     /locus_tag="AC16CB_03580"
                     /inference="COORDINATES: protein motif:HMM:NF023295.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR3341582.1"
                     /translation="MASTIDREAAVALMMEKYETCQRLFYLPSEAGQHCVYALLCENG
                     SLYIGQTSDLMKRFREHQAGRAADWTRRHKPLRIVHIETVDTRQEAMRLEYRWKTSSG
                     RKRLKRLAKAGNERLIKAVTELSQAFTLALPHPKALEIQDEMDFFRALSAAIQGDEPE
                     "
     gene            complement(7828..8466)
                     /locus_tag="AC16CB_03585"
     CDS             complement(7828..8466)
                     /locus_tag="AC16CB_03585"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR3341583.1"
                     /translation="MASQSYQLIMRTGPTPGKVFELDKSEIYLGRDVSNDIVVNDSEV
                     SRKHARMIMQAGGYILEDLGSTNGTFVGGQRLMGPHGLRPGEVVMLGENVTLAFEVGY
                     DPDATMVAAPAQMPARPPSQERQAQVPRAQQPAYTGQIPQGPAEPYYEGEVDEKKTNT
                     RTLILAGCGVLFIALCACIAGLFFIDQMRMWCEVPFKWFSFLYPLIGGAPCP"
     gene            complement(8645..>9016)
                     /locus_tag="AC16CB_03590"
     CDS             complement(8645..>9016)
                     /locus_tag="AC16CB_03590"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF046470.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HIR3341584.1"
                     /translation="PQADYVGYVRRLEQVIILTLARLGVSARQVEGLTGVWVDTQDSP
                     AKIAAIGVKVDARGVTRHGFALNVDPDMSYWEGIIGCGLVGYPVTCMANLLAEHPPMA
                     QVIDETILAFAQVFGYEMIEV"
BASE COUNT         1971 a         2398 c         2441 g         2206 t
ORIGIN      
        1 ctgtggtgtg tcttcggttg ctatggttgc atcgtccaac gctaccgggg catcatttac
       61 gcagactacc tcgattgtga cggcggcgat ggcgctcagt tccccatccg agatggtgta
      121 ggtgaagctg tcgctaccac agaagttggc attcggagag taagtgaatg ttccgttctc
      181 gtaggttagc gtgccgtttg atggttgggt aaaaccactg atgttcaggc tgtcgccatc
      241 gacatcgctg tcgttgccca gtaggtcgat cggattaatc accagcggtg tgtcttcggt
      301 tgctgtggtt gcatcgtcca gcgctactgg ggcgtcgttt acgggggtga cgtagatggt
      361 cacttcggcg gtgctgcact cattttcggc atcgcagatc tggtacctaa agctatcgta
      421 gccgttgaag ttctcattgg gggtgtaggt gaaggtgcca tcgccattgt tgatcaggct
      481 gccgttggca ggctcgctga tctgcaaagc gctggatggg accaggttgc cgtccacgtc
      541 gaagtcgttc atcgctacgt caactttcac cgatgtttct tcggctgtgg tcgcagcatc
      601 aggttctgct actggtgggt cattgatcgg caggacttcc acagtcacca tggcttcgtc
      661 acaggcgccg gagaggtcgc agacggtgta gttgaaggtg tcggtaccat accagttgga
      721 attgggtgtg taagtgaatg tgccgtcagc gttcagcacc accgagccat tggcgggctg
      781 ggtgtagctg ctgatcgaaa gggcatcgcc gtccgcgtcc gtgtcattgg gcagtacagc
      841 gattactacc gcctgatctt ctagggttgt cgcggcgtca tcatttgcca caggggcatc
      901 attgaccggg atcaccgtga tgatagccgt ggcagtgtca cacaaagccg gatcagcgct
      961 gtcgcaaacc tggtaggtga agctgtcggt gcctgaccag ttagtgttgg ggatgaaggt
     1021 gacgctacca tctgggttga ctgcaagcga accgttggct ggtggttcaa cgattgtaac
     1081 agagccggca tccaggttgt tctccgggtc gctgtcattg ccaagcacgt tgatattcac
     1141 ggcagtgtct tcctcggtgc ttgcgctgtc atcgctcgcc tcgggggcgt cattgaccgg
     1201 ggtaatggtg atgatcactg tggcagtgtc gcaggcgcca ctcggatcac acactgtgta
     1261 ggtgaagcta tcttcaccaa aaaagccttg ggcaggagcg taggtatagg tgccgtcgcc
     1321 attgtcgctt agtgtgccgt gttggggctg gctggcaatg ccggtgatgg tcaggctatc
     1381 gccatcggca tcgaggtcat tgagcagcag gtcgactatg ttgatcacca gcgatgtgtc
     1441 ttctaacgtg ctggctgtgt catccgttgc cactgggaca tcgtttgccg acgttaccgt
     1501 gatattcacc aaggcggtgt cacacaggcc gtaaacgtcg catacctgat aggaaaagct
     1561 gtcggagccg ttgtaatcag ggttgggggt gtaggtgaag gtgccgtcgc cgttgtttac
     1621 tagcgtgccg tacatcggcg cgtcgataac cgtcgcggtc gaggggtcta ggttgccgtc
     1681 cgcgtcgctg tcgttgcctg tgacaggaat agtcacagag ccgtcttcgg gaactgtggt
     1741 gctatcgtca acggcagccg gcgcgctgtt ggccgggccc atcacaaaaa cggtggcgct
     1801 gtcctggtcg ttgtaggata ccagcttctg accactctgg ttgttgttgc catttgcatt
     1861 ggcgatgttg gtgatggtgc cagccgaaga gagggttacc agggcgctgc ctgtagcaga
     1921 atcacctgcc agcagaccac cagaagtcag accgcttagc gagacggaga agtcatcgac
     1981 gctgtttgcc ggggtgccat tgtcatccac gagcgtaacg ttgtaaagct ctgctgtgcc
     2041 gggattactg accgtgtagc agtatttgac ggtatctcca gcattgacgg tcagggtctc
     2101 tgaacctggg caaatgccat ctgcggtagt gacggtcttg ctgatcgaga gcagcggttc
     2161 gcccgcaccc aaaagatcgc cgattttcaa gaaggggatg gtctgttgac cggtgttgat
     2221 cgtatcgctg accggggaaa ccacacgccc ggcgtcgagc tggccttgta ggttgccggt
     2281 tgggctggtg ccaggctggc aggagagtcg gacatcgatg cgcaggatga cctgctcgtt
     2341 ggcttcaaga tcggtcaccc gcacggtgcc gtggacttca ctaccggggg tgaagagcgt
     2401 tccgacgact gtctcgctga ccagggtggc aacactgcca ccgtcatcga tcagcccggc
     2461 atccgtgccg ccagctcctg ctccattgga gaccacgccg cggttaaccc tgacatcgat
     2521 gatatcagtg atcgccgcgc cagattgacc ggtgctatca gccaggaact tatagtcgaa
     2581 ctcagcgacc tggttggcat ggaccggttt ggcgctgatc ttgatggcgg tgaggaaagt
     2641 gaccacgtcg ccacaggcaa attcagagcc ttccaacgat tcgaccaggc cggtagcctt
     2701 gctgtttgag ccatcgtcat atgcaccgcc gccgatggca tggtcatacg aatacgggcc
     2761 ggaagcagca aaatcggtgt tagcgaaatc gccgcccgcg gcgtaagctg taatagtttg
     2821 aaaagctggt gcaatcacgc caaacaaaaa ggcgaacagc aaaataaacg actggccctt
     2881 tataaacaga cgttgattgc gagttaggtt aaacaagttg atcattatag tttgaatctc
     2941 caggtgtttt tatccatagc agaaatggat ttgttacata caggtagcat tgtaaccagc
     3001 ccataaccga ttgtctgtcg gagaagtttg atattttcag taaggattgt tagattttta
     3061 ttgatcatat gtttctgtgg ggtgggcagc gcggttgaga gctcaatttc ttgggccgat
     3121 gttccatcaa tcattgatgt caacagccga gagtagctca gtatctctgc cgataaatca
     3181 ttgataaatt ggacaataat tggtatattt attctgagca acgacactat ttcatcactt
     3241 caaaggagaa tttcaccatg aacccgcaag agaccctttc ccagcttgac cagctcatcc
     3301 attcccgctc gatcctgtgc cacccgttct acgtggcatg gcagcgaggt gaattgaccc
     3361 gcgaccagct cgctacatat gccgaggtgt attaccccca cgtggcatcc ttccccggct
     3421 atctcaaagc ggccatcgat gccgccgatg atgacggcgt gcgccaggag cttgagcgca
     3481 acctggcgga tgagatcgcc aatccgaaac cccatccgga actttggctg gatttcgccg
     3541 caggcttggg tctggaccct gccaatattg ccaacggagc ggcgcgacct gccgcaatcc
     3601 aaatggtgaa caccttcgac cggcttgccc agggtgatag tgcggcggca ttggcagcct
     3661 tatatgccta cgaaagccag cagcccgaag tctcgcggca gaagatcgaa ggattgcgct
     3721 tgcgttatgg tgtggacagt ctcgatgcgc tggcctattt cgaagtgcac gccgaagccg
     3781 acatccagca ccgggatggc gagcgccagg cgcttctacg ttgcctgaaa aacggcgtat
     3841 ctgcccagat tgtcctggaa tcagctgagc aggctttgga tgcttattgg ggattgctgg
     3901 atggcatttg tgaggaggcg gggataacca caggttaggc ctgcgactac cctcgcatgg
     3961 atgatgtggg ggatttttcg gagttttacc caaaaatccc cctatctcta gagggaatgt
     4021 gacggtttcc tgttctgaag gggcatcaat attcttttgc gcacctcatt tccataagca
     4081 gttccgctgc gacaagatcc aaagcgagat ttttcccgag ttcaagacct gctgccacaa
     4141 ctggttcgtc cgctacaagc ccgaggtgtt caagagcatt gtgtacctcc cttatgtttt
     4201 cgctaaatgc agcaggatga tacaagacca tccccaatag agctaatcct cgttcagttt
     4261 gtccttgttt ggttagtaac cagccaatat tttttattga aaacaaaacc cctggaattg
     4321 ctccaatagc tagggctata tccaatgctt cacatatgta cttccttgcc gcaggtaatt
     4381 tgtctaaacg gatcgatatg tctgccaaat tgctgagcaa tacggcttca aattcctgct
     4441 gcccaatctc acggacgagc gaaagtgcat tctggaaata agcctgggtt gccaccaggt
     4501 cgccacgttc ataggcgact gcacccaagt tgttgagagc gttagcctcc cggtcccggt
     4561 tgcctgcttg tcgcgctcgc tccagggtct cttcaaagaa tggttgtgct ttatccagct
     4621 ctcctttaac gagaggcacc gtccccaatc ggttaagggc gtataattcc aggtttagat
     4681 cacccagttg acgggctaga accaggcttt cttcacagtt gagacgcgcc tgatcaaagt
     4741 tgcccatacg ccattgtaaa tcccccaatc cgtacaaaac ttgcgctaat gtagccctat
     4801 caccggcaac ccgtgccagg ggcaggcttt cctctaagta ggcttgcgct tgttgataat
     4861 cacctccagc agaggcgact tggctgagcc agtataaggc atccgcactg cgcggccggt
     4921 catctaaatc ttgggcgatt ctctgtgcaa tttccagcgt ttccctagcg gcggaataat
     4981 cactcaactg gtaatacact ttccccagat gaagttgaat cggcaatcga tcttgatctt
     5041 ttcccgacaa caaatccaag gcctgtgtgt acatcgcccg agcttctccg aaggcgctga
     5101 ttcttagcgc gagatcagcc gctcggatgt accatctgac tgccttattt gctacccctg
     5161 ccagcgcgta atgcccagcg atcaaaccag catgctcatc tacccgctca ctgccctgtt
     5221 tctccaacca ggcagctacc tgggcgtggt aagcccgtcg atcgcgcttt aatacacttt
     5281 cataagttac ctcgtggagc atggtgtgcg cgaagatgaa ttccaccgtt ccgtcaaagg
     5341 ctgaaggctc atgccggaag atcatctcgc ggctacgcaa gatttgcagg atctggtgaa
     5401 gctccgagac gttggttatc gatcccgcct gattattctc aaacatttgc tctaaggtcg
     5461 tctcccagaa gatacgcccc actacggagg cgcgttgtag aacttcgcgc tcggtttggg
     5521 ataatcgatc aagccttgct tgcaagacgg cgatcaacgt tgggggaact cgcaacttgg
     5581 ccaacttatc gggctggatt cgccattctt gctccccttt gataatgacc ccatcctcaa
     5641 tcatcatcct caccaactct tcaacgtaga atgggatgcc atcggcggtg ccgattattg
     5701 ttttctccag cacctgtgga agatcgacca ctttccgcag gatttccaag accaagcggc
     5761 ggcattcttg cttcgataaa gggtgcagtt caatgcgact gtgattgtcg tccccttctc
     5821 cccaggtaga ccgttgttca aacaaagatg gctgggtcag gccaacaatc agcaaaggaa
     5881 atcccctcca attcctggac agatgctcta ccagatccaa tgaaccatca tccgcccagt
     5941 ggatatcttc taagagaatt atcaccgggt cctgcgctgc gactgcagaa aagaactggg
     6001 tggtgtaata gaaggcacgg ttgcgaattt gtttgacatc acctcggatg ccggcgatga
     6061 aaggactatc cgtaaagtcg aagcccagta actgcccgat gaagtgggat ttctcgattc
     6121 ccgcctcgcc cataaagtca acgaaacctg cttctagctt ctgcttggcg accgcggaag
     6181 agtcgctgtc ttgaatctgg aaacgcaatg aaaaaatatc gcgcgccaaa gcaaagggta
     6241 aactttgtgt ataccggaag gcccttccct taaataccca aaactcctcc ggaagcaagt
     6301 cgaaccagtt ttcaaactcg tacaacaggc gagttttgcc caatccagct tcaccaatca
     6361 ctgtgaccct atgcacctgg cgtttctcga tagcggagta tagggcatct tgtaaatgtc
     6421 tcaattctgc atctcgcccg accatgcgtg tttcaatacc ctcaaccccc cgggattctc
     6481 ggtagaaggc gcgagcttta atcctctgta ccaggtagac cagtattggt tcggctatac
     6541 ctttggcatg taaaggctca agggcttgca catcaaaaac gccacggata tgttgatagg
     6601 tgttgtgaga tatcaagaca ccaccagcag gagcagcatt ttccaagcgg gctgccacgt
     6661 tgaccgtcaa gcccatgatg gtatctcctg cctcagaaag gcccccaatt gctaccggac
     6721 ccgtgttaat tccaactcgg atattaaatc cactacgacc ccattcctgc tcaattttgg
     6781 cggcataaac ctcagctgct ttgagcaaac ccaacccggc tcgaacagct tgttcagcat
     6841 catcttctcg agcgacgggg gtgccaaaga cagccttgaa accatcaccc atgaagcggg
     6901 ttacatgtcc accatgctga tcaatgacgg ttgacaggta ctttagtgcc ccatccatga
     6961 tgtggatgat gtcttcagga tctaatcctt caatgagatg ggttgagtcg acgatatctg
     7021 tataaagcag ggtagccagc ttgagttgtt gttctgaggg aggttcaagc tcggctaatt
     7081 gcttgcgcag gccagcaatc gcaggatcga ccacggcgtc tccgagcacg tttcgctgtg
     7141 cttctaaagc agcaatggct tgttctagtt tttggcgttc gttcatctac ttctcttggt
     7201 aatttcccgg cgattttcaa gataatttta ttccggctca tccccctgga tggcagcgga
     7261 taaggcacga aagaaatcca tctcgtcttg gatctccagc gctttggggt ggggtagcgc
     7321 cagggtaaag gcttgcgaga gttccgtcac agccttaatc aagcgctcat tgccagcttt
     7381 tgccagtcgt ttgagccgct ttcttccaga gcttgtcttc caacgatatt ccaggcgcat
     7441 ggcttcctgg cgtgtatcca ccgtctcaat gtgaacgatg cggagtggct tgtgccgccg
     7501 cgtccaatca gccgcccggc cagcctggtg ctctcggaaa cgtttcatca ggtcggaagt
     7561 ctggccgatg tacaggctgc cattttcaca aagaagggca taaacgcaat gctggcctgc
     7621 ctctgacggc aagtagaaca gcctttggca ggtttcgtac ttctccatca tcaaagcgac
     7681 ggctgcttcc cggtcaatgg tggatgccat gcttaatcaa tgcctttacg aggcgggtgt
     7741 ccccatgccc aatgaatgca tccaactacc ctggatgtat aggatagagg caaggataat
     7801 ggttgttctg gagatggtgt gaaattctta tgggcagggc gccccaccga tcagcggata
     7861 gaggaagctg aaccacttga agggcacctc gcaccacatt ctcatctggt cgataaaaaa
     7921 caagcctgcg atgcatgcgc acagcgcgat gaacagcacg ccgcagccgg cgaggatcag
     7981 ggtgcgagta ttggtttttt tctcatcaac ctcgccttcg taataaggtt cagcggggcc
     8041 ttgcgggatc tgaccggtgt atgcgggttg ctgcgcgcga ggaacctggg cttgccgctc
     8101 ctgggatggc gggcgagccg gcatttgagc tggggcagcc accatggttg cgtcggggtc
     8161 atagccgact tcaaaggcca gggtaacgtt ctcgcccagc atgacaactt cgcctgggcg
     8221 caacccgtgt gggcccatca gccgctggcc accgacaaag gtgccgttgg tagagcctag
     8281 atcttccagt atgtatcccc cagcttgcat gatcatgcgg gcatgcttgc gcgagacctc
     8341 cgaatcgtta accacgatat cgttgctcac gtcacgacct aagtagattt cactcttgtc
     8401 gagctcgaat acttttcccg gtgttgggcc tgtgcgcatg attaattggt aggattgtga
     8461 agccatttat tcctcctcgc aaaggttggc ttgaagcccg ttgatcggta aagcaatctg
     8521 atcaggctct cctgctcggt tcaatatgat gaatttacat aataagagag atcttttccc
     8581 tcaactgcat aaggcctcag tatgctcata gtgtactgag ttttggggtt tgtgtcaaac
     8641 ggaattaaac ctcgatcatc tcgtatccga atacctgagc aaacgccagg atggtctcat
     8701 caatcacctg tgccatcgga gggtgttcgg cgagcagatt cgccatgcag gtgaccgggt
     8761 agccgaccag cccgcagcca atgatccctt cccaatagct catgtctgga tccacattca
     8821 aggcgaagcc atgtcgggtc accccgcgcg catctacctt aaccccaatg gcggcgattt
     8881 tggcgggcga gtcttgggta tccacccaaa ccccggtcag gccttccacc tgccgggcag
     8941 atacgcccag gcgtgccagg gtcaggatga tgacctgctc cagcctgcgc acatagccga
     9001 cgtaatctgc ctgggg
//
LOCUS       DBNGSX010000104         9969 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_7693, whole genome shotgun sequence.
ACCESSION   DBNGSX010000104 DBNGSX010000000
VERSION     DBNGSX010000104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9969)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9969)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9969
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_7693"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..677
                     /locus_tag="AC16CB_03595"
     CDS             180..677
                     /locus_tag="AC16CB_03595"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SixA phosphatase family protein"
                     /protein_id="HIR3341585.1"
                     /translation="MKTLLILRHAKSSWKNKALSDHDRPLSPRGQRDAPNVGKRLRSE
                     DLIPDAILSSTAKRARKTAEAVAEESGCAGELQLSRELYGGGPEAYLEAIRSLPSLVE
                     CALVIGHNPDLEELLEILSGESVRLPTAALAHLQLDIQDWQDLNEEEQGKLVNLWTPR
                     ENPFL"
     gene            1124..1621
                     /locus_tag="AC16CB_03600"
     CDS             1124..1621
                     /locus_tag="AC16CB_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR3341586.1"
                     /translation="MFADPEELRIAMRQWATGVTIVSAQHNGRRHGMAVSSFTSVSLD
                     PPLVLVSLEQVTKTHKLVMQAAYFGVTILDQNQRDISNRFAGRLTERSDRFSDLETFT
                     LISSAPLLAQGLAWFDCHVVSTYKAGNHTVFIGEVLAVKSVDAGKPLIYFDRDYRSLE
                     ADTAG"
     gene            1626..2201
                     /gene="amrA"
                     /locus_tag="AC16CB_03605"
     CDS             1626..2201
                     /gene="amrA"
                     /locus_tag="AC16CB_03605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HIR3341587.1"
                     /translation="MVVTLSPDERRILLGLARQAIEDAVLDRPITPLDLESLPEALRQ
                     PGVTFVTLTIHGGLRGCIGALEAYQSLAEDVREHAVGAALQDYRFPPVNENELKDLKI
                     EISRLTPPQALIYETPEELLERLRPGVDGVILRDGPRRSTFLPQVWMKLPQPADFLSH
                     LCAKMGANPNLWCEKTLQVFTYQVEEFHEVE"
     gene            complement(2203..2469)
                     /locus_tag="AC16CB_03610"
     CDS             complement(2203..2469)
                     /locus_tag="AC16CB_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HIR3341588.1"
                     /translation="MKMIIAIIGDHDNESVSQGLIQSDFRVTRIASTGAFLRRGSSTL
                     MIGVEDDKVDEAIQIIRERTTSTGETGVKRATLFVLNIEHYSQI"
     gene            complement(2473..2868)
                     /locus_tag="AC16CB_03615"
     CDS             complement(2473..2868)
                     /locus_tag="AC16CB_03615"
                     /inference="COORDINATES: protein motif:HMM:NF017920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HIR3341589.1"
                     /translation="MNEKYLKVVRLPDMERGAERIEPSVDRLMFAVVQAQDVRKASRA
                     LNAAGLLVAQLTSTGGFLGRRNVTLLIGMRENQEELAVKLIHDKCRQRVEYVSTPLEG
                     APLPIPVATPVTVGGATVFTFMVEHYEEI"
     gene            complement(2933..5311)
                     /locus_tag="AC16CB_03620"
     CDS             complement(2933..5311)
                     /locus_tag="AC16CB_03620"
                     /inference="COORDINATES: protein motif:HMM:NF046269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGH1-like glycoside hydrolase domain-containing
                     protein"
                     /protein_id="HIR3341590.1"
                     /translation="MRKWNLKAGDPLALTLMADARLGPTDYCDDQIWELRIGGGDPPA
                     LALQTTFGLRARSVRVFPRFGEGDTMVTAPAEFAAPPVVTNFFPNYLSVQCSPLSDID
                     VELEYWVPQSHAVAGRVGLMNNSSKARLIRLSLVAQLNPTEGQRMAPIEMQNAPALAG
                     MTGGLTPVMFVTGGPQAVGSPYPALNLEIELPPRASRRFTWCHAALQDVETSFSLARQ
                     MVACPWEAEIARVELVNSAQLEIYTGDPDWDAVFAFSQKLAAATFVGPTQHLPFPSIV
                     LSRQPDQGYSLRGDGGDYNHLWNGQPPLEIYYLAGLLLPGGTSLLQGLVRNYIAVREE
                     DGGLDWKPGLAGQRSGLMAMPILATLSWEIFQISQDRVFLEDVFEPLLKFIYRWFDDR
                     HDRDGDGVPEWDHPMQAGFEDHPVYSRWHEWSQGVDISSAESPALCALLHRECQALIQ
                     MGQILERVESLPVLQSYADHLHTALDAAWAPRRHSYLNWDRDSHYSTRGEKLGEITGP
                     GEVLIQRQFDHPVRLFVRIKTADENTRHPDLFIYGESASGQPRVERVPNEMFQWFLGQ
                     GSLTGERVYAKVERVKVQGIDQNDRMSLFSVGYSRPDHTVLLPLWSGIPDSKRALRIV
                     GKTILAERRYWRPYGIPACPDDAPNGHDQVCRCAHLPWNNLIGEGLLGYGYQEEATEL
                     ISRLMAAIIRNLKQNGAFSRYYHVETGEGLGERNVLTGLAPLNLFLSTLGVRIYSHEK
                     VFLSGSNPFPWPVTVKYRGLTVLRRADSTVITFPDGQTATITDPSPQMVTLD"
     gene            5576..6040
                     /locus_tag="AC16CB_03625"
     CDS             5576..6040
                     /locus_tag="AC16CB_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019550919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HIR3341591.1"
                     /translation="MSPLPSTPRGMYFEEFQAGQKFITAARTITEADIVNFAGLSGDY
                     NQIHTDAEYSKNSPFGQRVAHGLLGLSTVSGLAVQTGIVEGTIMAWREINEWKFIQPV
                     LIGDTIHAELEIIETKALRRIGGGAVVLDIAVKNQNGQTVMKGIWTFLVASK"
     gene            6053..9586
                     /locus_tag="AC16CB_03630"
     CDS             6053..9586
                     /locus_tag="AC16CB_03630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR3341592.1"
                     /translation="MTEDQSTPPEDKNEGKGRFRRLISDSDNEEEKLIEELLAKNKSQ
                     SETLPLDVGEEAKGTDDIPAFLRKRAEMEGEEDQFPSIPPFVDMEGAITYPYPAEELE
                     NEHAAAESPLLAEEEAQPPESEPSPSATETQPIDDGETKDNQPLPQRKSDTPPPPPLG
                     RAPRVSSPAVGTQGLPLPRRVDEIDISATRVSPSAYAQQRRGTPSRPIRPPGTPSGRP
                     GGPTSPRRVATPPRRPPPRPPAPQIDLRQGMGCFLRIAIPTLFVLVVISLCLGSYLLF
                     QYNRIKRGLPEVASLRQKASQFETTRILDRNGNVLYEILDPNAGRRTYVPLDKISPYM
                     LAATVATEDKEFYNHPGYDVFAIVRAFWQNYQSGETVSGASTITQQLARNLLFTPEER
                     SLRSYDRKLREAILASELTRVYTKDEILELYLNENYYGNLAYGVEAASQTYFGISADK
                     LTLAQASFLAGLPQAPAVYDVYTNRELTLNRQQAVLELMYLASQEQGCIDVSNSPQPV
                     CIDPVSAVSAANELRDYSFRPPEVQIRYPHWVTFIRTLLESQYDPQTIYRSGFSVYTT
                     LDPGLQDQAERVVREQVDRLADRHATNGALVAIRPTTGEILAMVGSADFYNASISGQV
                     NMALSPRQPGSSIKPITYVAAFERGWTPSTLIWDVSSEFTPSGKPNDPGPVYKPVNYD
                     DRFHGPVTVRSALANSYNVPAVKTLNFVNIYDDPTTPAQDGFIAMARRLGITTLLRPD
                     YGLSLTLGGGEVSLVELTGAYAVFANGGRRVPPVAITRILDHLGNQVYQYEPPQGEQV
                     IRSEHAFLISSILSDNQARTPAFGSNSLLNLPFQAAAKTGTTNDFRDNWTLGYTPDVA
                     VGVWVGNADYTPMQNVSGITGAAPIWNEFMQVAILQLTGNRPMPFTRPAGVIEKVICA
                     VSGAEASQWCPRQTTELFAADQPPLPKEQDLWQKVVIDTWTGLKASASCSDFTDERFV
                     LNVTDPWAKKWIREIKDGQDWAEEMGFEDPVTFTPERECKSDDPRPKLAFIKPTNNET
                     INTSPLEIVVVADASRNFRSFRLDYGVGENPVEWDTLEQRDSTVPQASEIYEWDLNDI
                     PRGIVTLRLEMKSSEDTEADLRVKINIQVPTPTPTPTQTPTPTLTSTLTPTSTNTPTI
                     TSTPTETLTPTPTATVTQTPTPTATPITPGP"
BASE COUNT         2493 a         2812 c         2467 g         2197 t
ORIGIN      
        1 caccactgaa gagaaaaatc aaggagtttt gctgagatta tgatggttgg tccacgaatg
       61 tccccggagc tgggactcta tagctcaaag aacgatgcgg cctgagagtg ttgtaggtat
      121 ttgccatcta tctgatcgtt tctacccatc tgatacaatt gatcagcaga tcttcttgaa
      181 tgaaaacctt acttatcttg cgccatgcca aatcgagctg gaaaaacaag gcgctttctg
      241 accacgatcg cccgctaagt ccgcgcggcc aacgagacgc acccaacgtt ggcaagcggt
      301 tgcgaagcga agatttgatc ccagatgcca tcctcagctc aaccgctaaa cgcgcccgca
      361 agacggcaga agccgtagca gaggagagcg gctgcgcggg agagcttcag ctatccagag
      421 agctttacgg aggcgggcct gaagcttacc tggaagccat tcgtagcctg cccagcctgg
      481 tggagtgcgc cttggtcatt gggcacaatc ctgatctgga ggagctgcta gagatactat
      541 cgggagaaag cgtccgcttg cctactgcgg cgctggcgca cttgcagcta gatatccagg
      601 attggcagga tctaaacgag gaagagcagg ggaagctggt caacctgtgg acgccgcggg
      661 aaaatccctt tttataaagt aaaggccggc ggctcctcaa aatgggggcg ccctgtgctc
      721 tcataactaa cccaagttgc caccttgggt gaatgatgat aaaagtccat gtgtgtctgg
      781 taaagttaat ggtacccaaa ccaaaacaaa ccggagcaac acatggacac ccgaattgta
      841 gcagtttatt gcgtttgtga cgatatgttg aaagccctgc cccaaaggag tggctaaaat
      901 ccagccttac tacccaaatg gtgtaaaatt gatacacacc gaggagttgt acagcgaaag
      961 gttttgacct agcgaggtcg atctacccaa ttgatggaaa attgatgtac agcggcagca
     1021 ctatctcata cataaccgcc acaggtgggg ttatacgtta aacttacaac catctaagac
     1081 gattaaatga tcaaatataa atcgcataat acaagttgaa gccatgtttg cagaccctga
     1141 agaactccgc atagccatgc gccaatgggc aaccggggtg actattgtct cagcgcagca
     1201 caacggtcgc cgtcacggga tggcggtcag ttcctttact tcggtgtcgc tcgacccacc
     1261 cctggttctg gtatcgctag aacaggttac caaaactcac aaactggtca tgcaagcggc
     1321 ctatttcggg gtcaccatac tggatcaaaa ccagcgagac atatccaatc gttttgccgg
     1381 gcgcctgacc gaacgcagcg atcgttttag cgacctggaa actttcaccc tgatctcctc
     1441 tgcaccgttg cttgcgcagg gattggcctg gtttgattgc cacgttgtat ctacatacaa
     1501 ggcgggtaac catactgtat tcatcggcga agtgctagcg gtaaaaagcg ttgatgccgg
     1561 taaaccctta atatacttcg accgcgatta tcgcagcctg gaggctgata ccgcaggtta
     1621 gtgcaatggt cgtgacgctc tcaccagatg aacgcaggat cttgcttggg ctggcgcggc
     1681 aagcaatcga agatgctgtg ctggataggc ctatcacgcc cctcgacctg gagagtctgc
     1741 cggaagcgct gcgccaaccc ggcgtgacct ttgtcaccct gacgattcat gggggtctgc
     1801 gcggctgcat tggcgcgctg gaggcgtacc agtcgctagc tgaagacgtg cgcgagcacg
     1861 cagtcggtgc tgctttacaa gactatcgat ttccccctgt caatgaaaat gagctcaaag
     1921 atttaaagat cgagatttct cgtctcaccc cacctcaagc cttaatctat gaaacacctg
     1981 aagaactgct ggaacggctc cgaccgggag ttgatggagt catcctacgt gatgggccac
     2041 gccgctctac cttcctgcca caggtctgga tgaagcttcc acagccagcg gatttcttga
     2101 gtcacttatg cgccaaaatg ggggccaatc caaatctctg gtgcgaaaaa actttacagg
     2161 tatttaccta ccaggttgaa gagttccacg aagtcgaatg acctatatct gggaataatg
     2221 ctcaatattt aagacaaaca aggtcgcccg cttaacccct gtttcgccag tcgaggtggt
     2281 acgctcgcgg atgatctgaa tcgcctcatc cactttatca tcctccaccc caatcatcaa
     2341 cgtgctggag ccgcgccgca agaatgctcc ggttgaggcg atgcgcgtca cgcggaagtc
     2401 ggattggatc aacccctgcg aaaccgactc attatcatga tctccgatga tggcaatgat
     2461 catcttcatg gcttaaatct cctcataatg ctcaaccata aaggtaaaaa ctgtggctcc
     2521 acctacagtg actggcgtgg caaccgggat cggcaacggc gctccttcca gcggtgtgga
     2581 aacatactcc actcgctgac ggcatttatc atggataagt ttgaccgcca gttcttcctg
     2641 gttttccctc atcccaatca gaagggtgac attgcgccgc ccaaggaatc cacctgtgct
     2701 ggtaagctgc gccaccaata aacccgctgc attcagagct cggctagcct tccggacgtc
     2761 ctgcgcctga acaacggcaa acattaagcg atccacagac ggctcaattc tttcagctcc
     2821 gcgttccata tccggcaacc ttaccacctt tagatacttt tcgttcatag atgtcacctg
     2881 tattatctat gcaatcaaat atgccaagag aatttttttg atctcaaacc agttagtcca
     2941 gggtaaccat ctgcggagac gggtccgtaa tcgtggctgt ttgaccatca ggaaaggtaa
     3001 tcaccgtgct atctgcccgt cgcagcactg tcaaccctcg atattttacc gtaactggcc
     3061 agggaaatgg gttagaaccg gatagaaaga ctttctcgtg agaatatatg cgcacaccca
     3121 gggtgcttaa gaaaagattc aacggagcaa gtccagtcaa tacgttacgc tcgcccaacc
     3181 cttccccggt ttcaacatgg taataacgtg agaatgcacc attctgtttg aggttacgga
     3241 taattgctgc catcagacgc gatataagct cagtagcctc ttcctgatag ccatagccca
     3301 gcaaaccttc tccgattaaa ttattccacg gcaaatgcgc acagcggcag acctggtcgt
     3361 gaccatttgg cgcgtcatct gggcaggctg gaatgccata tggtcgccag taacgccgtt
     3421 cggccaatat ggtctttccc actattctca gagcacgctt cgaatcgggt atacccgacc
     3481 ataagggtaa tagcacagtg tggtctgggc gagaatagcc cacactaaag agagacatcc
     3541 ggtcattttg gtctatcccc tgcaccttga cgcgttccac cttggcatag acccgctcac
     3601 cagtcaagct gccctggccc agaaaccact ggaacatctc gttggggacg cgttcaacac
     3661 ggggttggcc tgaagcgctc tctccatata taaaaagatc tggatgtcgt gtattttcat
     3721 cggcggtttt tatgcgcaca aacaggcgta ccgggtggtc aaattgacgc tggataagca
     3781 cctcgccagg accggttatt tcacccagtt tttccccgcg ggtactatag tggctgtctc
     3841 tatcccaatt caggtaggag tgacgcctgg gtgcccaagc tgcatccaaa gccgtatgca
     3901 ggtgatcggc ataagactgg agcacgggta aagactcaac ccgttcgaga atttgaccca
     3961 tctggatcaa ggcctgacat tcgcgatgca atagcgcaca cagcgccgga ctctccgcag
     4021 atgagatatc gacgccttgc gaccattcat gccaccgtga gtacacaggg tgatcttcga
     4081 agcctgcctg catgggatgg tcccactcgg gaacaccatc cccatcccgg tcatgacgat
     4141 catcgaacca acgatagata aacttaagca gcggttcgaa aacatcttct aaaaacacac
     4201 gatcctggct aatctggaaa atttcccagc tcaatgtggc caggataggc atggccatca
     4261 ggccgctgcg ttgtcccgca agacctggtt tccaatctag accgccgtcc tcttcccgga
     4321 cagcgatgta atttcgtacc agcccttgca gcaaagacgt cccacctggg agcaataatc
     4381 ccgccaggta ataaatctcc agcggtggct ggccgttcca caggtgattg tagtcgcccc
     4441 cgtcaccacg tagagaatat ccttgatcgg gctgtcgtga taaaacaatt gaagggaagg
     4501 gcagatgctg tgtaggaccg acaaaagtcg ccgcagccaa tttctgagaa aaggcaaaaa
     4561 ccgcatccca atcgggatca ccagtataga tctctaactg agcggaattg accaattcga
     4621 cgcgtgcaat ttctgcctcc caggggcagg cgaccatctg gcgggcgagt gaaaaagagg
     4681 tttcgacatc ttgcaaggca gcatggcacc aggtgaatcg tcgcgaggct ctgggtggca
     4741 attcaatctc caggttaagc gctgggtaag ggctccccac cgcctggggt ccgccagtga
     4801 cgaacattac cggcgtcagc ccgcctgtca tcccagccaa cgcgggagca ttttgcatct
     4861 cgatcggagc catgcgctgc ccttccgttg ggttcagttg ggcaactagg ctaaggcgta
     4921 tcaggcgtgc cttggagctg ttgttcatca gtccaaccct gcccgccacc gcatgggatt
     4981 gaggaaccca atactccagc tctacgtcta tatcggaaag aggagagcat tgcacgctca
     5041 gataatttgg gaagaaattg gtcacgactg gaggggcagc aaactctgct ggagcggtta
     5101 ccatcgtatc tccctcgcca aaacgcggga acacacgcac cgagcgggca cgcagcccga
     5161 aagtggtttg cagtgccaga gcaggcgggt cacctccccc tatcctgagt tcccaaattt
     5221 gatcgtcgca ataatctgta ggccccaggc gagcgtcagc catcaaggtc aacgccagcg
     5281 gatcgccagc tttcaggttc cattttcgca tggtacattg tattgtatgg tatgttgccc
     5341 taaaggtcaa acggatcagg gcaatcgcat ttgggcgatt tcccaccaat gaggagagaa
     5401 aatggggtgt tttggtgggg gtatacccac caaaacaccc caaacactct ttttgcggga
     5461 ttgcgcatga ccctcagggc atatgcaatt gccctgtcaa acggattaca gttcatgatt
     5521 ttatgctaga atgggtaaaa ttatccgcac gctttgctat cttcaaggag ccttcatgtc
     5581 ccctctccca tccacgcctc gcggtatgta ctttgaagag ttccaggctg gccaaaaatt
     5641 catcactgcc gcgcgcacca ttacggaggc agatattgta aactttgcag gcctctctgg
     5701 cgattataac cagatccata ccgatgccga gtacagcaaa aactcaccct ttggtcagcg
     5761 ggttgctcac gggctgctgg gattgtccac cgtttctgga ttggcagtgc aaaccgggat
     5821 cgttgaaggc actatcatgg catggcgaga gatcaacgag tggaaattta tccagcccgt
     5881 tcttatcgga gacacaattc acgccgagtt ggagatcatt gagacaaagg ctctccggcg
     5941 catcggcggt ggggcggttg tgctcgatat agcagtaaaa aatcaaaatg gccagaccgt
     6001 gatgaaaggc atttggacat ttttagtcgc ttcaaaatag gtagtaattt atatgaccga
     6061 agatcagtct actcccccag aagataaaaa cgaaggaaag ggtcgtttcc gacgtttaat
     6121 ttcggattca gacaatgaag aagagaagct tattgaagag ctgctcgcca aaaacaagtc
     6181 ccaatccgag acccttcctc tggatgtagg tgaagaagca aaggggacag acgacattcc
     6241 agccttcctg cgaaaaagag cagagatgga aggggaagaa gatcaatttc ccagtatacc
     6301 ccccttcgtc gatatggaag gggcgattac ctatccttac ccagccgaag aactcgaaaa
     6361 cgagcacgcg gccgccgaat ccccgctgct agcagaagag gaagcccaac caccggagag
     6421 cgagccctct ccatccgcaa ccgaaaccca gcccattgat gatggagaga ccaaggacaa
     6481 ccagcctctc ccccaacgaa agtcagatac gccaccaccg ccaccattag gacgcgcccc
     6541 tcgtgtctct tcccctgcgg tgggcaccca gggattgccc ctgccgcgtc gcgtggacga
     6601 gatcgatatt agtgcaacgc gtgtctcgcc ttccgcctac gctcaacaac gcagggggac
     6661 tcccagcaga cccatacgac ctccagggac cccatccggt cgaccaggag ggccaacctc
     6721 cccaagacga gtagcgaccc ctccgcgccg cccaccccct cgccctcccg ctccgcagat
     6781 tgacttgcgc caggggatgg gctgttttct gcgtatcgct atccccactc tctttgtgct
     6841 ggtggtcatc tcgctctgcc tgggttcata cctgctcttt caatacaacc gcataaaaag
     6901 agggctgccg gaagtggcct cgcttcgcca gaaagcatcc caatttgaga ccacgcgcat
     6961 tctggatcgc aacggcaacg tcctttacga gatactggat ccgaatgccg ggcgacgcac
     7021 ctatgtaccc ctagataaga tctctcccta catgctggct gctacggttg caacagagga
     7081 caaagagttt tacaaccatc caggctacga tgttttcgcc atcgtgcgtg ccttctggca
     7141 aaactaccag agcggtgaga cagtttcagg tgcatccacc atcacacagc aactggcgcg
     7201 caacctgctc tttaccccag aggagcgctc ccttaggagc tatgatcgta aactgcgcga
     7261 ggcaatactg gcttctgagc tcacccgtgt ctacaccaaa gatgagatcc tggagttgta
     7321 cttaaatgag aactactatg ggaatctggc ctatggcgta gaagctgctt cccaaaccta
     7381 ctttggtatc tccgccgata aactgacctt agctcaggca tccttcttgg ctggtctgcc
     7441 tcaggcgccg gctgtatacg atgtatacac caaccgcgag ttgactttaa atcgccagca
     7501 agccgtgctg gaattgatgt acctggcaag ccaggagcaa ggctgcattg atgtcagcaa
     7561 cagccctcaa ccagtgtgca tcgatcctgt atctgcggtc agtgctgcca acgaactgag
     7621 ggattactct tttcgacccc ccgaggtgca gatccgctac ccacactggg taacgtttat
     7681 acgcactttg cttgagtcac agtacgatcc acaaacgatc taccgctccg gattctcagt
     7741 ttacaccact ttggatccag gtctgcaaga tcaggctgag cgggtcgtgc gcgaacaggt
     7801 agatcgttta gcagaccgtc atgctaccaa tggcgcattg gtagcaatcc gacctacaac
     7861 cggtgaaata ctggcgatgg taggctctgc cgacttctac aatgcctcca tatctggcca
     7921 ggtgaatatg gcccttagcc ccaggcagcc cgggtcatcc attaaaccaa tcacctatgt
     7981 tgctgccttt gagagagggt ggacaccttc cacgctgatc tgggacgtgt cttccgagtt
     8041 caccccctcc ggtaagccca acgaccctgg tccagtgtac aagccggtca attacgatga
     8101 tcgattccat ggcccggtca cggttcgttc cgccctggca aactcttaca acgtgcctgc
     8161 agtaaagacg ctaaacttcg tcaacattta cgatgatccg accacccctg cccaggatgg
     8221 ctttatcgct atggcgcgcc gcctgggcat caccactttg ctacgccccg attacggcct
     8281 ctcgctcacc ttgggaggcg gggaagtctc gttggtggag ttaaccggtg cttatgcggt
     8341 cttcgccaat ggcgggcggc gtgtcccacc agtagccatc acccgcatct tagatcacct
     8401 gggcaatcaa gtgtatcaat acgaaccccc acaaggggag caagtcatcc gcagcgagca
     8461 tgccttccta atctcctcga tcctctcgga taaccaggcg cgcactccgg cttttggatc
     8521 caactcactc ctcaacctgc cattccaagc agctgctaag accggcacta ccaacgattt
     8581 tcgggacaac tggacactgg gttatacacc agatgtcgcc gttggggtat gggttggtaa
     8641 cgccgattac acccctatgc agaatgtaag cggcatcact ggtgctgcgc caatctggaa
     8701 cgagttcatg caggttgcca tcctgcaact caccggcaac aggccaatgc ccttcacccg
     8761 tcctgcagga gtgattgaaa aggtgatttg cgcggtatct ggcgccgaag cctcgcagtg
     8821 gtgtcccagg cagacgacag aactcttcgc cgcagatcaa cctcccctgc ccaaggagca
     8881 agacctttgg cagaaagtgg tcattgatac ctggactgga ttgaaagcct cggcctcttg
     8941 cagcgatttc accgacgagc gtttcgtctt aaacgtgacc gatccttggg ctaaaaaatg
     9001 gatccgtgag atcaaggatg ggcaggattg ggctgaggag atgggctttg aagatccggt
     9061 cacttttaca cctgaaagag aatgtaaaag cgatgacccg cgaccaaagc tcgcctttat
     9121 taaacccact aacaatgaaa ccatcaatac cagccccctg gagattgtgg ttgttgcaga
     9181 cgccagccga aatttcagat ccttccggct ggattatggc gtgggggaga accctgtgga
     9241 atgggataca ttggagcagc gcgattctac cgtccctcaa gcaagcgaaa tttacgaatg
     9301 ggacttgaac gatattccac gcgggatagt aacattgcgc ctagaaatga aaagtagtga
     9361 agacaccgag gctgatcttc gcgtcaaaat caacatccag gtacccacgc cgacacccac
     9421 cccgacgcag acgcctactc ctactttgac gtctacactg acaccaacat ccactaacac
     9481 acccactata acctctaccc ctaccgaaac gctcaccccc acaccaacgg caacggtgac
     9541 ccaaacccca acgccaacag caacacccat tactccgggc ccataggata cgcacaaaaa
     9601 gcaaaaagcc aaccaatggt tggcttttta taacactcgc ccgagtgtca gatatatatt
     9661 agatcaatgc tgtcccaatt gcaatactct atttgtacta ttttttgact atttttggag
     9721 cgccgtgctg aagttaatgt atggggaaac tagccggtga attaacaaca tgacaactcc
     9781 aggcgggaac cttatccatg cctatccccc gagtctcacc aatctcgatc tcctgtccag
     9841 ggagaggcgg gagccatagt gatcttcgcc atctccagca atacatggtc ataattcacc
     9901 cgccagccgg caaaatccat ccatgcctgt tcacggttcg cagtgagggg caccccctgc
     9961 gcagccaac
//
LOCUS       DBNGSX010000105         3204 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_7759, whole genome shotgun sequence.
ACCESSION   DBNGSX010000105 DBNGSX010000000
VERSION     DBNGSX010000105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_7759"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..680
                     /locus_tag="AC16CB_03635"
     CDS             282..680
                     /locus_tag="AC16CB_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR3341593.1"
                     /translation="METLTLELQTMYGDHHVLEVRRLMLEMPGVEDVYASSGFRVVEI
                     AYDPAKIAPEALRSKLSEAGYLGDLPVPVEAGAAVYVEKGDRSFFRHTTVYENTQQVV
                     SFAQNVGYSGRPLWPCPGMGPIQAKNLEEE"
     gene            683..2698
                     /gene="cooS"
                     /locus_tag="AC16CB_03640"
     CDS             683..2698
                     /gene="cooS"
                     /locus_tag="AC16CB_03640"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0043885 - anaerobic carbon-monoxide
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006091 - generation of precursor
                     metabolites and energy [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HIR3341594.1"
                     /translation="MARARRSYEEQSSDPAAQEMLIHADELGLSTAFTRADDMVPCNI
                     GAAGMCCKLCGMGPCRLTKDGQTGVCGATIDTIQARNLIRAIAAGSAAHSDHGRDMAF
                     TLKAVANGEAEGYVLRDIAKLRTVAAEYGIPIEKRSPQEIANDLADLYIAQFGQQKGS
                     ISPVIRAPKKRQKLWEEQGVIPRGIDREVVEALHRTHIGDDQDPEHILEHAIRTGLAD
                     GWGGSLIATDVSDILFGTPAPILGQANLGVLKDDMVNVVVHGHEPTLSQMIVAATQDP
                     EIIEYAKAAGTNGVNVSGICCTANEILMRQGIPAAGNFLHQELAILTGAVEAMVVDVQ
                     CIMQALVELASNFHTMVITTSPKVKIKGSVHIEFNEHKALTIAKQILKTAIDNYKKRG
                     NTRIPQVREDLIPGFSHEYINYMLGGSYRASFRPLNDAIMAGRIRGVAAIVGCNNPRS
                     KQDYMHTYVTQELLKQDVLVVETGCGAIAAAKLGLLLGEAGLDKVGPGLKEVCEAIGI
                     PPVLHMGSCVDNTRILTVLTQMVEEGGLGDDIDQIPAVGLAPEWMSEKALSIATYVVA
                     SGGYVIFGGSSPVSGMPDRMSDSDIILRYISEGWEKIYGGKLEFIQEADEMIRRTLEH
                     IDKKRAALGLPEYNPDNFGRSGDWRMLEVENLPLSERRAAIYGVAAD"
     gene            2773..>3204
                     /locus_tag="AC16CB_03645"
     CDS             2773..>3204
                     /locus_tag="AC16CB_03645"
                     /inference="COORDINATES: protein motif:HMM:NF036895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341595.1"
                     /translation="MSRYIATRAIRGANALVSEAELMLQKAIREKGIETPVSFPNTAY
                     FLPLTLGMTGVAVENLGDLKPVLDHARNLLHPTPSVKHWTPYLGETLDSGMATLLAAE
                     TIEAVRFVYNLQPEAMPGFQLAGGTAFTSPDAGANGGGNGMD"
BASE COUNT          833 a          697 c          882 g          792 t
ORIGIN      
        1 aggcaaatag aataaaaaag agacgaaatg atcaatatcc agacaacttt actaataaat
       61 atagtaataa atgtcacttg tctgtctaga tcatttttgc tatagtggat tgcgtaattc
      121 atacatccat ccccattgga gcagttttta tggcaggtaa acaatcgagc cagcctgaat
      181 ttcacctgta gtaattgcta taggtgtgaa tccaggcaca tttttgtatg gaagtaatta
      241 tttcatcaac gaaatcaaaa aagagataag gaaggagaat catggaaact ttgacactcg
      301 aactacaaac catgtatggt gaccaccacg tgctcgaagt aaggcgcctg atgcttgaaa
      361 tgccaggtgt tgaagatgtt tacgcaagta gtggatttcg agtagtcgaa attgcatatg
      421 acccagccaa gatagcacca gaagctttgc gctccaagtt aagtgaagca gggtatcttg
      481 gagatttacc ggttcccgtg gaagctgggg cagccgtgta tgtggaaaaa ggggatcgtt
      541 ccttcttccg acatacaacg gtttatgaga acacccaaca ggtggtaagt tttgcgcaga
      601 atgttggtta ttctggtcgg cctctttggc cttgcccggg catgggccca attcaagcga
      661 agaatttaga ggaggagtaa aaatggcgag agctaggcgg tcatacgaag aacaaagttc
      721 agatcctgct gcgcaagaga tgctcattca tgcggatgaa ctcggtctga gcactgcgtt
      781 cacccgcgct gatgacatgg taccttgcaa catcggtgcg gcgggtatgt gttgtaaatt
      841 atgtggtatg ggtccatgtc ggttgacgaa agatggacag acaggtgttt gtggggctac
      901 cattgacact attcaagcgc gcaatcttat ccgtgctatt gctgcaggat ctgcagcgca
      961 ttctgatcac gggcgcgata tggcatttac cctcaaggcg gtagcgaatg gtgaagcaga
     1021 aggttatgtg ctgcgtgata tagccaaact gcgtaccgta gctgcagaat acggcatccc
     1081 gattgagaag cgctcaccgc aggagatagc caacgaccta gcggatctct atattgccca
     1141 attcggacag caaaagggaa gtatttctcc tgtcatccgc gcaccaaaga agcgccagaa
     1201 gctatgggaa gagcaggggg ttataccgcg cggtattgat cgcgaggttg tggaggcttt
     1261 acatcgtacc cacattggcg atgatcagga tccagaacac atcctagagc atgctattcg
     1321 caccggcctt gcggatggct ggggcggttc gttgattgca acagatgtat cagatatttt
     1381 attcggtact cctgccccga tcttaggcca ggcgaacttg ggtgtattga aagatgatat
     1441 ggtcaatgtt gtggtgcatg gtcatgagcc aaccctgagc cagatgattg tggcagcgac
     1501 ccaggaccct gagatcattg agtacgcaaa agctgctggg acgaatggcg tcaacgtctc
     1561 gggtatttgc tgtacagcca atgaaatctt gatgcgccaa ggtatcccgg cggcagggaa
     1621 cttcttgcat caagaattgg cgatattgac tggtgcggta gaagctatgg ttgtggatgt
     1681 gcagtgcatt atgcaggcat tggtggaatt ggccagcaat ttccatacaa tggttatcac
     1741 tacttctccg aaggtgaaaa ttaaaggttc ggtccacatc gagttcaacg agcataaagc
     1801 tttgacgatc gcaaagcaga tattgaagac cgccatcgat aattacaaga aacgcggcaa
     1861 cactcgtatt ccgcaggtcc gtgaagattt gatcccgggc ttctcgcacg agtacataaa
     1921 ctacatgtta ggtggttcat atcgggcttc cttccgcccg ttgaatgatg ccatcatggc
     1981 ggggcgcatt cgaggcgttg cagctattgt cggctgcaat aacccgcgca gcaaacagga
     2041 ttacatgcac acctatgtta cccaagagct gctcaaacaa gatgtcttgg ttgttgagac
     2101 gggatgtggg gcgattgccg cagccaagct gggcctgctc ctaggcgagg ctggtttgga
     2161 taaagttggg ccaggcttga aagaggtatg tgaagccata ggtattccac ctgtattgca
     2221 catgggatct tgcgtggata acacccgcat tttgaccgtc cttactcaga tggttgaaga
     2281 gggcgggtta ggtgatgata ttgatcagat acctgcagtt ggcctagcgc cggagtggat
     2341 gagtgagaaa gcgctctcta tcgccaccta cgttgttgca tctggaggtt atgtgatatt
     2401 cggtggttcg tccccagtga gcggaatgcc cgaccgtatg tcagatagcg atattatctt
     2461 gcgatacatc agcgaaggct gggaaaagat ttacggcggc aagcttgaat ttatccagga
     2521 agcggatgaa atgatccgcc gcacacttga gcatatcgac aagaagcgag ctgcgcttgg
     2581 gttgcccgaa tacaatccgg ataactttgg gcgtagtgga gactggcgaa tgctggaggt
     2641 agagaacttg ccactctccg aacgccgcgc agcaatctat ggtgttgcgg ccgattgaga
     2701 aacagcctgt attgtgcggc ttcactgaat atgagaaaaa tgtccacaat acgatagcga
     2761 ctcggaggaa aaatgtcacg atacattgca acccgcgcaa tccgtggtgc gaatgcatta
     2821 gtttctgaag cggagttaat gctccaaaag gctattcgtg agaaaggaat tgagacacca
     2881 gtttctttcc caaatacagc ttattttctc cctttgacct tagggatgac cggggtggct
     2941 gtagagaatc tgggcgatct taagcctgtt ctcgatcacg cgcgtaacct attgcatccc
     3001 acgccgtcag tcaagcactg gacaccttat ctgggtgaaa cacttgacag tggtatggcg
     3061 acgctgctgg cagccgaaac gattgaggct gtgcgttttg tttataacct tcagcctgaa
     3121 gccatgcctg gcttccagct agctggtggg actgccttca ccagcccgga tgctggtgct
     3181 aatggtgggg gtaatggaat ggat
//
LOCUS       DBNGSX010000106         1601 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_492747, whole genome shotgun sequence.
ACCESSION   DBNGSX010000106 DBNGSX010000000
VERSION     DBNGSX010000106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_492747"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="AC16CB_03650"
     CDS             complement(<1..560)
                     /locus_tag="AC16CB_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341596.1"
                     /translation="MTTKLGYLTHRRPRALIFSLVSYLLWVTVTIRWITEFIEASHPL
                     TWLVSGMLVLYGILMGLEPLITKGSTWRAHLYLALQTALVMVAMLFYFELDFFAILLL
                     PLCGQAVLIFPRRAASIWVIIFVLANIVGQIHQFGWPQAISFILLYFGAMFFVAAFSF
                     LTVQAEEARQKSEELLGELREAHKKL"
     gene            772..>1601
                     /locus_tag="AC16CB_03655"
     CDS             772..>1601
                     /locus_tag="AC16CB_03655"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3341597.1"
                     /translation="MFRKSIISQMSLVMIAVLTVLAPLSTGYAHPSDGTSIYTLDGAS
                     SESESIESFVDQYVADGMQAAHVPGFAVVVVKDGKTLISKGYGYADLEQRIPMTPQTN
                     LRAGSVSKPVVATAVMQLVDQGLVDLDAPISRYIPDLELTDRFGQGSTVAQLLTHQSG
                     YTDADLLSHAPTLEEWQPLGEYLHENLGPRDIQPGVMAYSSRNTALLGYMIEKITGLP
                     YDQAIARSLFDPLGMSKTTFTQPLPDEIRANLATGYFYQGGTYEIVPLDYVRSSPGIA
                     "
BASE COUNT          425 a          394 c          408 g          374 t
ORIGIN      
        1 tgcagtttct tgtgagcctc ccggagttct cccaatagct cttcgctttt ttgacgcgcc
       61 tcctcagctt gaactgtgag aaatgaaaat gcagccacga aaaacatagc tccaaaatat
      121 agcaggataa aagaaatagc ttgaggccag ccaaattgat ggatctgccc cacaatgttg
      181 gccaagacga agataatcac ccaaatggac gcagcccggc ggggaaatat taataccgcc
      241 tgcccgcaga ggggtaacaa gaggatcgca aagaagtcta gctcaaaata aaagagcatg
      301 gcgaccatca ccagggctgt ttgtaaggcc aggtatagat gggcgcgcca ggtagagccc
      361 ttcgtaatca agggttccaa tcccatgagg atgccataga gtaccagcat accgctgacc
      421 agccaggtca gtgggtggct ggcttcgatg aactccgtaa tccagcgtat tgtgacggtc
      481 acccatagca ggtaacttac gagcgagaat ataagggcgc gtggtcggcg atgagtaagg
      541 tagcctaatt ttgttgtcat gtgtgcacat ccaattccga aggaaaccca attgaaatca
      601 ttatatgaac aaatatcccc cgcatcaata cataaaatta tggatatggg ggtctgggag
      661 ataaatagtt ttatggatgt ccatttccag gctttttcgg atgacaacta aggtgaactt
      721 gggcatactg agatcaaaga tttcagcaaa ccaaatatac ggaggtgtgc aatgtttaga
      781 aagtcaataa tctctcaaat gtccctggtc atgattgctg tactcactgt gctggctccc
      841 ctctcaactg gttacgccca tccttcggat ggcactagta tctacacctt ggatggggcc
      901 tccagtgaat cagaatctat cgagagcttt gtagaccagt atgtagccga tggcatgcag
      961 gcagcccacg ttccgggttt tgcggttgtc gtggtgaaag atgggaaaac cctgatttcc
     1021 aagggttacg gctatgccga tcttgagcaa cgaatcccga tgaccccgca aacgaacctg
     1081 cgcgccggat cggtgtcaaa accagtcgtt gccacggctg tcatgcaact ggtcgatcaa
     1141 ggcttggtgg atttggatgc ccccatcagt agatacatcc cggacctgga actgactgat
     1201 cgatttgggc aaggtagcac ggtagcgcag ttgctcacac atcaaagcgg ttacacggat
     1261 gctgacttgc tctcgcatgc cccgaccctc gaagaatggc agccgttagg cgagtatctg
     1321 cacgagaatc taggtccgcg cgatatccag cctggtgtga tggcttacag cagtcgaaac
     1381 actgcgctgt tgggctacat gattgaaaaa atcaccggtc ttccctatga ccaggccatt
     1441 gcgcgcagcc ttttcgatcc cttgggcatg agcaaaacga catttacaca gccactacct
     1501 gatgagatca gagccaatct ggcaacaggc tatttctatc agggtggcac ttacgagatt
     1561 gtgcccctgg attatgtacg ttcctcgccg ggcatcgcta t
//
LOCUS       DBNGSX010000107         5798 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1462432, whole genome shotgun sequence.
ACCESSION   DBNGSX010000107 DBNGSX010000000
VERSION     DBNGSX010000107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5798)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5798)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5798
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1462432"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            708..1382
                     /locus_tag="AC16CB_03660"
     CDS             708..1382
                     /locus_tag="AC16CB_03660"
                     /inference="COORDINATES: protein motif:HMM:NF046475.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HIR3341598.1"
                     /translation="MNQSERKITALKAQKRNRSRVSVYLDGEYAFGLARIVAAWLQVG
                     QVITDEKIAELKAQDAGEVAYQQALRLLNYRDRSESEIIRSLKQRQTPEEIITEVIAR
                     LRRNGLLDDQRFAKDWVENRTEFRPRSSRALSYELQEKGVEQETIQQVLESFDEDEAA
                     YLAAKKYVRRIENLEWPEFRQRLLGYLARRGFQYETTRAAIARIQAEFKMNESTQNTL
                     LEEVDR"
     gene            1379..2911
                     /gene="rny"
                     /locus_tag="AC16CB_03665"
     CDS             1379..2911
                     /gene="rny"
                     /locus_tag="AC16CB_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HIR3341599.1"
                     /translation="MNGIWFWIPITLIALLIGGAIGYYLKRILIEKELEKDKAKANLI
                     LEESQKRANELELRAKNQALEIRQTAEAEIARRRGEINREEDRLQKRREEVDMRSDRL
                     EKREQALNKRQSAVDRRTNEIERLHSEQVEELQRISQMSLDEARNQLLSEVEKESRND
                     MARIIRQIEAEAREQGEQRARLIITGAIQRVASEHVSEVTTSVVPLPADEMKGRIIGR
                     NGRNIHAFEQVAGVDVIVDDTPEAVTISCFDPVRREIARRALAKLVMDGRIHPAHIEK
                     VLKNEQKEVERDIVDAGEQATYDAGVAGLHPEVIKTLGRLKYRTSYGQNQLAHAVETA
                     RIAAMIASELGAEVEVARAGALLHDLGKAIDHNMEGTHAQIGADLARRCGVNPKVVNA
                     IASHHHEIDQETVEAAIVEAADAISGARPGARRESLEQYIKRVRALEEIANSFKGVNQ
                     SYALQAGREVRIFVKPEEIDDLDSVRLARDIARNIEDSMQYPGQIKVTVIRETRTIDY
                     AK"
     gene            complement(3040..3636)
                     /locus_tag="AC16CB_03670"
     CDS             complement(3040..3636)
                     /locus_tag="AC16CB_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3341600.1"
                     /translation="MTNCYLVADPDTGEAAVIDPADNGRTIVGEAERRGWRIGNIWLT
                     HAHFDHLAGASGVADHTNPPPPVALHPEDYPLWRMQGGAMLFGMRIDPGPEPTVDLKH
                     GMILRLGGNQFEVRHAPGHTRGHVILYCAEEKVVFCGDVIFQGSIGRTDLPGGDFNTL
                     IQSIRTQILTLPDDTRLLSGHGPETTVGQERRFNPFLL"
     gene            complement(3684..4406)
                     /locus_tag="AC16CB_03675"
     CDS             complement(3684..4406)
                     /locus_tag="AC16CB_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341601.1"
                     /translation="MQSIRSHLSLTSLAILMLVMHLSACATTGAPPAMAPEASLPTLN
                     PTDVASAPLLIPTQAPAILEARRLNLEWPPTIRVGDSDVIRLTLEVDDQGNLTPTAQI
                     EGHETRGETVFIPDLYDTHNVRAEARLDMSGMQVTPEGEVSEPLAPGQSVSFFWSVRP
                     VDVGTYRGTIWMHLRFIPLDGSAESRKPLTAQLVEVRAVNFLGLGGLAARLLGVLGTL
                     VGSVLGLDNIIPWVIKRFRRKK"
     gene            complement(4423..4602)
                     /locus_tag="AC16CB_03680"
     CDS             complement(4423..4602)
                     /locus_tag="AC16CB_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341602.1"
                     /translation="MGFRKLRRILGILILLLSLALLIWGLWPYTELTRSTIIQPGEMQ
                     LPTPESFMWMAQYVI"
     gene            4725..5126
                     /locus_tag="AC16CB_03685"
     CDS             4725..5126
                     /locus_tag="AC16CB_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341603.1"
                     /translation="MKLSQLFTVNAIVFISLGIAFALYAPLMLGMFGIQESEGSTVMY
                     WYTTSFARLLGAALFGFGFVLWALRRQSTLPDVRKGVSLALTISYTLGFVVAITQQAS
                     IWGTLYGWITAGVFLVLTAGYAYILVKKNSE"
     gene            5134..>5798
                     /locus_tag="AC16CB_03690"
     CDS             5134..>5798
                     /locus_tag="AC16CB_03690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014283858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3341604.1"
                     /translation="MNDFPDIHPSRPELNLLTPSGWVDYALLDSGNGLKLERYGPYTF
                     VRPEPQAIWQPAASQESWQAAHAIFQPTREESGGHWQFNKPVKESWVMQYKGLKFRVQ
                     TTAGRHLGVFPEQAPHWDWIGNVVHTAKRDINVLNLFGYTGLASLEAARAGAHVTHID
                     ASKKSVAWARENQALSDLGNKPIRWIVDDALKFVQRDARRGVKYDGLILDPPKFGRGP
                     KGEV"
BASE COUNT         1518 a         1464 c         1519 g         1297 t
ORIGIN      
        1 ggcgaaacta tcaaattgat aacgcaatct tactaggtca tccgcaacta atatgacata
       61 ttatatctgg tttacttggc atgatgtaaa aacaagaaga atatgacctg cgcagatgaa
      121 ggaaaagagg gattttggat gatttcacct atccagatcc ccgaaattcg gtatagtgca
      181 aggtattttt ttcatttaga ataaatttgg ggtgaataaa agatttccca ttatagtgaa
      241 ggctataagc ccaacaaatc ttgtgtgatt ttctatgcac ataatgatat aatacctaac
      301 aatatagacc attaataaat cattggttgc attaagaaat gtaacccagg ttctcctgct
      361 gatttttgat cgatcacggc tttggaatct aacgcttgaa aaagcttgtc tcagaaaaaa
      421 tgattgtcct ggtgtatttt cgaactggaa taagtgacta taaatttagc gcatgtgact
      481 gcggccggcc ttatatcctg tgccggaact cgagacctta tcgccattgc caatcattga
      541 gatagatgtg cttgacaggt taataatttg ctgtaaatag acctttgtgt ggtctacatg
      601 gcacgtttcg cctgtcgtgt catataactg agatatagaa tatcccccaa gcagccgcga
      661 ttgatcaagg tgtgatcacg gctgtttttt attagatacg cgatttgatg aaccaatcgg
      721 agcgaaaaat caccgcccta aaagcccaaa agcgcaaccg ctctcgagtt agtgtctacc
      781 tcgacgggga atatgctttt ggccttgccc gcatcgttgc cgcctggtta caggtcggac
      841 aggtgataac cgatgagaag atcgcggagc tcaaggcaca agacgctggc gaggtggcat
      901 atcaacaagc gctccgattg cttaattatc gcgaccgctc tgaatccgag attatccgca
      961 gcctaaagca acggcaaact ccagaagaga tcataacgga agtgatcgct cgcttgcgca
     1021 gaaatggact gctggacgat cagcgctttg cgaaagactg ggtggagaac cgtactgaat
     1081 ttcgtccacg cagtagccgc gcattgtcgt acgaattgca agaaaaaggt gttgagcagg
     1141 aaaccatcca gcaagtgctt gagtccttcg acgaggatga ggcagcatac ctggctgcta
     1201 agaaatatgt tcgcaggata gaaaatttgg aatggcctga attccgtcaa aggttactag
     1261 gttatttagc ccgccgtggt tttcaatatg aaaccacccg cgctgccatc gcacgtattc
     1321 aggctgaatt taagatgaat gaatctacac agaatacctt acttgaagag gttgatagat
     1381 gaatggaata tggttttgga ttccgattac cctgattgct ttgctgattg gtggtgcaat
     1441 cggttactat ttgaagcgaa tcctgattga aaaagaactt gaaaaggata aagctaaagc
     1501 aaatctcata ctagaagaat cccaaaagcg cgccaacgag ttggagctac gcgccaagaa
     1561 tcaagctctg gagattcgcc aaaccgccga agctgaaatt gcacgccgta gaggtgagat
     1621 caatcgcgag gaagaccggt tgcaaaaacg tcgcgaagaa gtggacatgc gatccgaccg
     1681 gctggagaag cgtgagcagg cactcaacaa gcgccagagc gctgtggatc gacggaccaa
     1741 tgagatagag cggttgcatt ccgagcaggt agaagaattg cagcgcatct ctcaaatgag
     1801 cttagacgaa gcgcgcaacc agctgctttc cgaggtggaa aaagaatctc gtaacgacat
     1861 ggctcgaatt attcgccaga tcgaagccga agcgcgcgag caaggcgaac aacgagcccg
     1921 cctgatcatc accggtgcta tccaacgcgt ggcctctgag catgtttccg aagtgaccac
     1981 atcggttgtg cctctgcctg ccgacgagat gaaaggacgc attatcgggc gcaacgggcg
     2041 caatatccac gccttcgagc aggtcgctgg agttgatgtc atcgtggatg acacgcccga
     2101 ggcagtgaca atttcctgct ttgatccggt gcggcgcgag attgccaggc gggcactagc
     2161 caaactggtt atggacggtc ggattcaccc cgcccatatc gaaaaagtct tgaagaatga
     2221 gcagaaagaa gtcgaacgag atattgtcga tgccggagag caggccactt acgatgctgg
     2281 tgtggcgggt ttgcatccag aggtcatcaa aaccctagga cgtttaaaat accgcacttc
     2341 ctacgggcag aatcagctcg ctcacgcggt ggagactgcc cgcatcgccg ccatgatcgc
     2401 gtctgagctg ggcgccgagg tggaagtcgc cagggcaggc gctcttctcc acgacctggg
     2461 taaagcgata gatcataata tggaaggcac tcacgctcaa attggcgctg atttagcacg
     2521 ccgctgcggt gtcaacccga aagttgtcaa tgctatcgcc tcccatcacc atgagatcga
     2581 ccaagagacc gttgaagctg ccattgtcga ggcagcagat gcgatctctg gagctcggcc
     2641 tggagcgcgg cgagagagcc tggagcaata cattaaacgc gtgcgtgccc tggaagaaat
     2701 cgccaactcc ttcaaaggcg tcaaccagag ctatgccctg caggcgggtc gtgaggtgcg
     2761 catctttgtc aagcccgagg agatagacga cctcgactcg gtgcgcctag cgcgcgacat
     2821 cgcccgcaac attgaagatt ccatgcagta tcccgggcaa atcaaagtga cggtcatccg
     2881 agagacccgc acgatcgatt acgcaaagta gagaccgctg cgcaggtcat ccagcaaaag
     2941 aataatatgc tgtccaaaaa atatttggat ggcttggaac gcaaaccaga gtaggtcggg
     3001 tgccatacct gacctacttt tctttcacct aagaaatcat cacaacaaga agggattgaa
     3061 gcgccgctcc tgcccaacgg tcgtttcagg tccatgccct gaaagcaggc gcgtgtcgtc
     3121 tggcagggtg agtatctggg tccggatgct ttggataagc gtattaaaat caccccctgg
     3181 caaatcagtg cggccaatgc tgccctggaa tatcacatca ccgcaaaaaa ccaccttttc
     3241 ctcagcacaa tacaatatga catgcccgcg cgtatgacca ggagcatggc ggacttcaaa
     3301 ctggtttcca cccaagcgca agatcatgcc atgcttcaaa tccaccgtcg gctcggggcc
     3361 cgggtcgatg cgcatgccaa acagcatggc ccctccttgc atgcgccata gtggataatc
     3421 ttcggggtgc agggcaaccg gtggcggcgg gttagtgtga tctgcaaccc cactcgctcc
     3481 tgcaagatgg tcgaagtgtg catgagtaag ccagatattt ccaatgcgcc aaccgcgcct
     3541 ttctgcctca ccgacaatgg tacgaccatt atcagccgga tcaatcaccg ctgcttcacc
     3601 ggtatcagga tcagctacca gatagcagtt ggtcattaca gagcccagag taaatgagac
     3661 tatcgaaagc atagcgatct ggtttacttc tttctacgaa atctcttgat cacccatggg
     3721 atgatattat cgagcccgag cactgatccc accagggtcc ctaatacgcc cagcaacctg
     3781 gctgccagtc cacccaagcc taggaagttt actgctcgta cttcaacaag ctgggcagta
     3841 agcggtttac gactctcagc gcttccatcc aggggaataa agcgcaaatg catccagatc
     3901 gtaccacggt aagtacccac atcaaccggt cgcacactcc agaaaaatga aaccgactgg
     3961 cctggggcta gtggctcact cacctcccct tcaggtgtca cctgcatacc tgacatatcc
     4021 aagcgcgcct ccgcccgtac attatgcgtg tcgtaaaggt ctgggataaa gactgtctct
     4081 ccgcgcgttt cgtgaccttc aatttgcgcc gtgggagtca ggttgccctg atcatccacc
     4141 tcgagggtca ggcgaataac atccgagtca cctacccgga tcgtaggagg ccattccaag
     4201 ttcaggcgac gcgcctccaa aatggctggg gcttgagtag ggatcagtag tggagcagaa
     4261 gccacgtctg ttgggtttag agttggcagt gacgcttcag gggccatcgc tgggggagca
     4321 ccagttgtgg cacatgcgct caggtgcatc acgagcatca ggatagccaa actggttagg
     4381 ctcagatggg acctgatact ctgcatggca atcgatatcc agctagatga catactgagc
     4441 catccacatg aaactctccg gggtgggcag ttgcatttcc cctggctgga tgatggtaga
     4501 gcgggtcagc tcagtgtaag gccataatcc ccagatcaga agtgccaggg aaagcagcag
     4561 gatcaaaata ccgagaatcc gccttaattt tcggaagccc atggctgcta ttatacaaca
     4621 gcaaaccatc cattaggccg ctaaagtatt ctaaaataca tcgaagcatg ctacactgta
     4681 catgccacca attggtaatt attaaccaaa cgaaggaatg tcacatgaaa ctcagccaac
     4741 tcttcacggt caatgccatt gtatttatta gcctgggtat cgccttcgcg ctgtacgcgc
     4801 cgctgatgct tgggatgttt ggtattcaag agagcgaagg aagcaccgtg atgtattggt
     4861 acacgacctc gtttgcccgc cttctcggtg cggccttgtt tggatttggc ttcgtccttt
     4921 gggctttacg ccgtcaatct actttgcccg atgtgcggaa gggggtcagc ctggctttga
     4981 cgatctcgta caccttgggg tttgtggttg ccatcacgca gcaggcatcc atctggggga
     5041 cactctatgg ttggatcact gcaggggtat tcctggtact gaccgccggt tacgcttata
     5101 ttctggtaaa gaaaaattcc gagtaagcat ttgatgaacg actttccaga tatacacccg
     5161 agcagacctg agctaaactt gcttaccccg tccggctggg tggattacgc cctgttagat
     5221 agcgggaatg ggctaaaatt ggagcgctat ggcccataca ctttcgtgag acccgaaccg
     5281 caggctatat ggcagccggc agcatcccag gaaagctggc aggcggctca tgcaatattc
     5341 caacccaccc gcgaggagag cggcgggcac tggcagttca acaagccagt caaggaaagc
     5401 tgggtgatgc aatataaagg gttgaagttt cgcgtccaga ccaccgctgg ccgccacctg
     5461 ggcgtttttc ctgaacaagc gccgcattgg gattggatcg ggaatgtcgt gcacactgcc
     5521 aagcgcgata taaacgtgct caatttgttt ggctacactg gcctggccag cctggaggcg
     5581 gccagagctg gagcgcacgt cactcatatc gatgcctcca aaaagtccgt cgcttgggca
     5641 cgagagaacc aggccctctc ggatctagga aataagccta tacgctggat tgtggacgac
     5701 gctctgaagt ttgtgcagcg cgatgccagg cgaggcgtca aatatgatgg tctgatactt
     5761 gacccaccca aatttggacg cggaccgaaa ggcgaggt
//
LOCUS       DBNGSX010000108         3609 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1323866, whole genome shotgun sequence.
ACCESSION   DBNGSX010000108 DBNGSX010000000
VERSION     DBNGSX010000108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1323866"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="AC16CB_03695"
     CDS             complement(<1..271)
                     /locus_tag="AC16CB_03695"
                     /inference="COORDINATES: protein motif:HMM:NF016206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HIR3341605.1"
                     /translation="MFFFYDYRYLMFMLPAFLLMLAVQWYVNSAYNKWGRVPARSRMS
                     GAQAAQRLIERGGLYDVKIEGVQGNLTDHYDPRTRVLRLSRGVYSG"
     gene            complement(361..1605)
                     /locus_tag="AC16CB_03700"
     CDS             complement(361..1605)
                     /locus_tag="AC16CB_03700"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR3341606.1"
                     /translation="MKSKALVTFISVIIAGILLTGACSAGFVAGRVFNPPEQILALLP
                     GQEISPPVAQEESGTPQELEELFVPFWQAWDLVHDQYVEQPVDDEVLMRGAIEGMLDS
                     LDDPHTSYLNPEQYQSLNAQLQGDESYEGIGAWVDTTSDFLTIISPIPGSPAEKAGLR
                     TGDKVIAVDGEDMTGIDGELVRQRVIGPAGSTVNLTILRTGQDPFDVQITRAEITIPS
                     VDYRMLEDDIAYVRVLIFADNTKDQLQDALKDLMSQNPQGMILDLRGNGGGYLDSAID
                     VASEFISEGVIAYEEYGDGTRDTYDARRGGLATDIPMVVLINEGSASASEIVAGAIQD
                     LGRGQLIGMTSFGKGSVQIPTALRNQQGAVRITVARWLTPKERTIHGKGLTPDVEVPV
                     TEEDFANNRDPQLEKAVEIISK"
     gene            complement(1835..2632)
                     /locus_tag="AC16CB_03705"
     CDS             complement(1835..2632)
                     /locus_tag="AC16CB_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013323.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HIR3341607.1"
                     /translation="MDKLITLEQAVGLVRSGDMLALGGITLYRRPIAFVRALIRRFFG
                     EAEPGDLTLLAFTAGLESDLLVGSGMVSRIRTCYFGLEIFGLAPMFTYFANRGEIEIM
                     EESEASLAFGLRAQMAGVGFMPGLGWLGTDLPKLRPDVLTVTDPYNGEELIAFPAIHP
                     DVAVIHALQADPHGNAQIGDNKGIDEELALTARTVIVTAEEIVPEMEKANLVAPLVHY
                     IVHASHGALPTSCHPLYPLDGQALLAYVDKVSEPESCRAFLESWIAE"
     gene            complement(2680..3342)
                     /locus_tag="AC16CB_03710"
     CDS             complement(2680..3342)
                     /locus_tag="AC16CB_03710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HIR3341608.1"
                     /translation="MTYSTLFFDLDDTLYPPQNGVWTAIRERMNLYMRDKMRLPSEQI
                     PDLRRYYYETYGTTLRGLQIHYQVDPDEFLAFVHDLPIRKMVQPDPKLRDLILSLPQR
                     KYIFTNADSAHADRVLDALGMQGCFDGKIDVRALDFHCKPEPLAYQTALSLAGETLPQ
                     RCVYLDDSPRNLAPARKLGFFTILIGDDDIHTAADLAMRLPHDLPRLMPELWQENHRH
                     PG"
BASE COUNT          857 a          923 c          967 g          862 t
ORIGIN      
        1 tcccggaata tacgccccgc gagaggcgca gcacccgcgt gcggggatcg tagtgatcgg
       61 tcaagttgcc ttgtacgccc tcgattttta catcatataa gcccccgcgc tcgatcaggc
      121 gctgggctgc ctgggcaccg ctcatacggc tgcgggcagg taccctgccc cacttattgt
      181 aggctgaatt tacataccac tgtactgcca gcatcagcag aaatgctggc agcatgaaca
      241 tcaaataacg gtagtcataa aagaagaaca cagtatacct ccttaccaga tacaggcttg
      301 atatagcctg aaatactcac atatcattag ttgtgtattg atttaattga caaaagtcaa
      361 tcatttgcta attatttcta cggctttctc caactgcggg tcgcgattat tggcgaagtc
      421 ttcttcggtg acgggcactt ctacatcggg tgttaaacct tttccgtgga ttgtgcgctc
      481 tttgggtgtt agccagcggg ctacagtgat tcgaaccgct ccctgttggt tgcgcagagc
      541 cgtggggatc tgcaccgatc ctttgccgaa ggaggtcatt ccgatgagct gccctcgacc
      601 caggtcttgg atcgcaccgg cgacaatctc tgaggctgag gcgcttccct cattgattag
      661 caccaccatg gggatgtccg tagccaggcc tccccgtcgg gcatcatagg tatcccgcgt
      721 accatctccg tactcctcgt aggcgataac cccctcggaa atgaactccg aggcgacatc
      781 aatggcagaa tccagatagc caccgccatt gccccgtaag tctagaatca tgccctgtgg
      841 attctgggac atcagatctt tgagcgcatc ttgtaattga tctttagtgt tatcggcaaa
      901 gatgaggacg cgcacatagg cgatatcgtc ctccagcatg cggtaatcca cacttgggat
      961 agtgatctct gcgcgtgtga tctgtacatc gaaagggtct tgaccggtgc gcaagatggt
     1021 caggttaacc gtgctgcccg ctggaccaat cacacgctgg cgcactagtt cgccatcgat
     1081 accggtcata tcttcaccat ccacggcgat gaccttatcc ccggtgcgca aacctgcttt
     1141 ctccgctggg gaaccgggga tgggactgat aatggttagg aaatcgctgg tggtgtctac
     1201 ccacgcgccg atgccttcat aggattcatc tccctgaagt tgggcattga gagactggta
     1261 ctgttctgga tttagataag aagtgtgcgg atcgtccagg gaatccagca tcccctcaat
     1321 tgcgccgcgc attaatactt cgtcatcgac gggctgttct acatactggt cgtgaaccag
     1381 atcccaggct tgccagaagg gtacgaagag ctcctcgagc tcctgtggcg tgccgctctc
     1441 ttcttgagca actggggggc tgatttcctg gccaggcaaa agggctaaaa tctgctcagg
     1501 gggattgaat actcgcccag cgacaaaccc cgctgagcag gcgcctgtca gcaatatccc
     1561 tgcgatgatc acactgataa aggttactaa agccttggat ttcatgagaa atctccacta
     1621 tctcttctaa aactcatccc gtaaattggt gagttggggt gatttgaaga cgcttctctt
     1681 atgcaatcac tgcactcacc gagcaataag gatacttttt catatcgtaa agattaccat
     1741 aatagcatta gaggattata aaaaatatta tcaagtctag aatgtggggt gaattaccac
     1801 actaccctcg cttctgcatt cacctatagt gagactattc agctatccaa ctttctagaa
     1861 aggcgcggca agattcaggt tcgctgacct tgtcgacata agccagcaag gcttgtccat
     1921 ccagcgggta aagcgggtga caagaagtag ggagtgcgcc atgtgaggcg tgcacgatgt
     1981 aatgcaccaa gggggctacc agattggctt tctccatttc tggtacgatc tcttctgcag
     2041 tgacgatcac ggtacgggca gtcagggcca actcttcgtc gatgccctta ttatccccaa
     2101 tctgggcatt accatgcggg tctgcttgca aagcatgaat aactgccaca tcgggatgga
     2161 tggcggggaa ggcgataagc tcctcgccat tgtaagggtc ggtcactgtg agcacatcag
     2221 gtcgaagctt aggcaagtcg gttcctaacc agccgagccc gggcatgaaa cccacgccag
     2281 ccatttgcgc ccgcagccca aatgccaggc tggcctcact ctcctccatg atctcaattt
     2341 caccgcgatt ggcaaagtaa gtgaacattg gcgccaggcc gaagatctcc agtccaaagt
     2401 aacacgtgcg tatacgcgag accatgcccg atcccaccag gaggtcgctt tccaggccgg
     2461 cagtaaaggc cagcagggtt aggtctcccg gttctgcctc gccgaagaaa cgacggataa
     2521 gagcgcggac gaaggctatt ggacggcggt agagtgttat gcctcctaag gctagcatat
     2581 cgcctgagcg caccaatcca acagcctgtt caagtgtaat gagtttatcc atatccctcg
     2641 gagctcttcg gtctttggta tcccttgctc aactcgtgct tatcctggat ggcggtggtt
     2701 ttcctgccac aactctggca ttagcctggg caagtcgtgg ggaaggcgca tggcgaggtc
     2761 tgctgcggtg tggatgtcgt cgtccccaat caagatcgtg aaaaaaccta gcttccgggc
     2821 tggagcaagg ttacgaggtg agtcgtcgag ataaacgcaa cgctgaggga gcgtttctcc
     2881 tgccagggaa agagccgttt ggtaggctaa aggctccggt ttgcagtgga aatctaaggc
     2941 gcgcacgtcg atcttgccat cgaagcagcc ttgcatgccc agtgcgtcca gtactcgatc
     3001 tgcgtgggcc gagtcagcgt tggtgaagat gtactttcgc tgggggaggc tgaggatcag
     3061 atcgcgaagc ttggggtcgg gttggaccat tttacggata ggcagatcgt gaacaaaggc
     3121 tagaaattca tcagggtcaa cttggtagtg aatttgaaga ccgcgcaggg tggtgccgta
     3181 ggtctcataa taatagcggc ggagatcggg gatttgctca gatggcaggc gcattttatc
     3241 gcgcatatag agattcatgc gctcccgtat ggctgtccat accccgtttt ggggtggata
     3301 gagtgtatcg tccaaatcga aaaacagcgt tgaataggtc acagggtaat tataaggcac
     3361 aggggaggtt aggcaaagat taatagatgg tgtgaatttg gttagccagc gtcacatttt
     3421 gctcaaaaat gcgactaata aggtagacaa acaaacataa gatcgctagg tgaggaccac
     3481 cgagataaca cacataagga gataccaaat gaaatccata catcgctttt tattccccct
     3541 gcttgctctg gctgcgattg cgctcagcgt cgctggtgta gccctcgctc gcgaaatgtc
     3601 cgaaaggga
//
LOCUS       DBNGSX010000109         2128 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_631706, whole genome shotgun sequence.
ACCESSION   DBNGSX010000109 DBNGSX010000000
VERSION     DBNGSX010000109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_631706"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..1072
                     /locus_tag="AC16CB_03715"
     CDS             320..1072
                     /locus_tag="AC16CB_03715"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3341609.1"
                     /translation="MAIVTGAGSPTGIGFATAKLLAEMGAKVAVAATGQRIHDRAAEL
                     LQVGHTARGYIADLTDRAATRSLVEAVESDFGKIDILVNNAGMTMEGAPEEFSEFAEL
                     SDEAWDLSIARNLTTCYNVTRLVIPGMIGRRYGRIINVSSVTGPLVSNRGEAAYSAAK
                     AAMIGMSRSIALEVAEHNVTINNVAPGWVATGSQTDEEKIASAHTPFGRAGTPTEMAT
                     MIAFLATPGASYITGQMMIVDGGNCLQESKGV"
     gene            1075..1572
                     /locus_tag="AC16CB_03720"
     CDS             1075..1572
                     /locus_tag="AC16CB_03720"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3341610.1"
                     /translation="MKTNIFAPDLFKDRVVLITGAAGGVGGATAELLGSLGAKLALTD
                     CKEEAVKEKAATLEALGVQADISSVADCQRVIATTLAHYGGLDVLVNCAGVWVEGDSA
                     EATEEDWERCVNVNLKGTFFMCSKAIPALKKSRGAIVNISSDAGVVGNVGAAVMLDFG
                     VTAGY"
BASE COUNT          505 a          504 c          621 g          498 t
ORIGIN      
        1 ggaagagctg gcgcgcatca aggacgaatt gccccccgtt gaggctcgcc tcagcggacc
       61 atcgaggctg gaagagctgg cacgcatcaa ggatgaggtg taccctacga ctactcgtct
      121 cagtgggtca tcccgactgg aagagctggc gcggatcaag gaacgctgat accttgaatc
      181 tgatctattc ctaacgccgt ttctctactt ctacccacag ctgcgcacca tccatggtcg
      241 gatcgatcca gccattgcga tcggtgcgca gcagggtatg gccttgcatc acttccagtg
      301 tctctagggt gggcaggccc tggcgatcgt gaccggcgcg ggcagtccaa cgggcattgg
      361 gtttgccacc gccaaactgc tggcggagat gggagcaaag gtcgctgttg ccgcgacagg
      421 gcaacgcatt cacgaccgcg ccgccgaatt gctacaggtg ggccataccg cccgcgggta
      481 catcgccgac ctgacggaca gagcagctac caggtcactg gtcgaagcgg tcgaatctga
      541 ttttggaaaa atcgatatcc tcgttaacaa tgcagggatg acaatggaag gagcgccgga
      601 ggagttcagt gagtttgcgg aactctcgga tgaagcttgg gatttatcta tcgcgcgcaa
      661 cctgaccact tgctacaatg tgacccgcct cgttattccg ggaatgatcg gtcgccgcta
      721 tggacgcatt atcaatgtct catctgtgac cggtccgctg gtgagcaatc gcggcgaagc
      781 ggcgtatagc gctgcaaaag cggcgatgat cggcatgagc cgaagtattg cattggaggt
      841 cgctgaacat aatgtcacga tcaataatgt agctccgggc tgggtggcaa caggctcaca
      901 aacggatgaa gaaaaaatcg cttcggctca tactccattt ggccgcgccg gtactccgac
      961 tgaaatggca accatgatcg cattccttgc aacaccaggt gcgagttata tcaccggaca
     1021 aatgatgatc gtggatggcg gcaactgtct gcaagaaagc aaaggagtgt aatcatgaaa
     1081 acaaatattt ttgctcctga tctcttcaag gatcgtgtgg tgttgattac aggcgcagcc
     1141 ggcggagtag gtggggcgac ggctgaattg ttggggagtt tgggagccaa actcgcgctc
     1201 accgattgca aagaggaggc ggtcaaagag aaagccgcga ctctcgaagc gttgggcgtt
     1261 caagcagata tttcatccgt ggcggattgc caacgcgtga tcgcaaccac acttgcccat
     1321 tacggtggtt tggatgtgct tgtcaattgc gcaggggttt gggtggaggg cgatagcgcc
     1381 gaggcaaccg aggaagattg ggagcgttgt gtcaacgtaa atctcaaagg cacattcttt
     1441 atgtgcagta aagccatccc tgcgttaaag aaaagccgcg gcgcaattgt gaatattagt
     1501 tcggatgcag gtgtggtggg aaacgtgggc gcggcggtga tgttggattt tggcgtcacg
     1561 gcgggatatt gaaactgctt tctcctcaac aaatcggttt cttactcggg gagcatgctg
     1621 agcacctctc gcgactggat gattggcgat ccttttaata ttggtacacc tacatcccgg
     1681 tgagcgccgc cgtagtgctt ttctcgacgg ggtggagcct ggtaggagat ggcttgcggg
     1741 atgccctcga cccgcggctg agggtcttga acgttggccc tatttcagtg tcttagcggt
     1801 tgcatagctg cgggcaagtt ccctaatcgc ccgcgcgctg aatgggcaga acacatcata
     1861 gggaccgtat gcccagtaag aactgggcgt aattgtttta agaattcatt gccgaatctg
     1921 ttgataacgc gagaaaaata ttgtaaagta cattatagta aagataaccg ttcttaaaaa
     1981 tatcatctct ggtgaatcct taaaatcgcc caattggtaa gatatgctgc aaatatgcag
     2041 tatatccaat agttgggcag ttcacattta ctggcttgat tttatccttt tttgcagtag
     2101 aatgagtttg tggagtaaat cacaagtt
//
LOCUS       DBNGSX010000110         5598 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_8190, whole genome shotgun sequence.
ACCESSION   DBNGSX010000110 DBNGSX010000000
VERSION     DBNGSX010000110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_8190"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(302..766)
                     /locus_tag="AC16CB_03725"
     CDS             complement(302..766)
                     /locus_tag="AC16CB_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012036844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HIR3341611.1"
                     /translation="MSHPCEITEQPAQPTLSIRTRAAVQNLPQVLGDGYGAIMQYLGE
                     LGMSPTGHPFVIYYNMDMQDLDIELGFPVSQPLPGKGQIHAARMEGGKVAEYLYVGPY
                     ADCGPAYEELAQFVKDQGYEATGVAIEYYLNDPSQPPYEEPQTRIVLPLKNT"
     gene            complement(938..3100)
                     /gene="fdhF"
                     /locus_tag="AC16CB_03730"
     CDS             complement(938..3100)
                     /gene="fdhF"
                     /locus_tag="AC16CB_03730"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014515597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HIR3341612.1"
                     /translation="MIKPDRVVSTTCPYCGVGCNLELHVKDETIYRVTSPFDAVVNQG
                     NLCVKGRFGYDFVYHRRRVTTPLLRKVRQEPGKRTQAFERDEWRQVSWDEALDYVADR
                     LVEIYRRDGPDALAVYCCAKATNEDNYLLQKMFRSLFRTNNVDHCTRLCHAGSVVALQ
                     QAVGSSAMSNTASEVIKSDVFIVTGSNTPENHPIIALQMKAAVMKHGAKLIVIDPRRS
                     ELCDFATLWLPLKPGTNVPVFSAMAHVIIRDGLVNRQFVEQRTEGFDEFARSLEKFTP
                     EYAEMVSGVKRDLIVQAAHMYASAERGAIYWGMGISQLSHGTASALAVIHLALLTGHI
                     GREGTGLNPLRGQNNVQGASDMGSMPYHYPGYMLVDNPDNAAKWERAWNIEPGGLSLK
                     RGLTTTEILSHARPGGVRSLYIMGENPMMSEPNLNLTRHHMEQLEFLVAQDLFINESG
                     AFADVILPSTSWAEKDGTFTNTDRRVQRVRQAIAPRGDSRPDWQIICDLAKRIEARLG
                     RPNSTGWDYAQPPEVLAEVGRVVPEFTGVTYERLEESGLQTPVWDESHPGTPYLFSQS
                     FPRGRGKFHALEFVPSVELPDDEYPFILSTGRVLEHWHGGTLTRHSKLDDLYPEARVE
                     LHPTDAALIDIRDGQAVRVSSRRGTVALRATVTEKTRAGVVFIPFHFAEAAANLLTID
                     ALDPQAKIPEFKACAVKIEPVSDADLPHPEAVLRRGRY"
     gene            complement(3173..3892)
                     /locus_tag="AC16CB_03735"
     CDS             complement(3173..3892)
                     /locus_tag="AC16CB_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024900.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR3341613.1"
                     /translation="MPEKVTLTIDGSSVTVPAGTTILKAAEQIGEKIPTICYHDHCTA
                     NALCRLCVVEVEGARVLSPSCVAQVSEGMKVETRSPKVERSRRTILEMLASAVDLSEA
                     PEILDFMGEYDVHDEPFPGSERRQPPLLDDNPMYMRDYAKCVLCWRCVQVCADDAQYT
                     YAINFSGRGFETSIGTFFDKTMPDTTCVFCGQCVGVCPTGALKPKREWLLEQGMTPDE
                     VMQMTRTERRGKRDSRVENQE"
     gene            complement(3983..>5598)
                     /gene="nuoF"
                     /locus_tag="AC16CB_03740"
     CDS             complement(3983..>5598)
                     /gene="nuoF"
                     /locus_tag="AC16CB_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HIR3341614.1"
                     /translation="GHLPEPVAAEVGRALGVPLADVYGVIDFYSMFYSHPTGKTMVRV
                     CTDPACALGGGEDVLERLCRQLNVSPDEPSPDGAFQVERAPCLGLCEHAPAALVGDRA
                     VGDAGSLQLDDLLSGHVSPLQAVLGGDLRLLTANCDNGQPTSLQEYAARGGFAGLKKA
                     LRMPPDSVIAEVKASGLVGRGGAAFPTGIKWEGAAKAEGSPKYVICNADESEPGTFKD
                     RLLLEGDPQLILEGMLIAAYATGASRGYIYIRGEYPHAYQIMEDALSQARQAGYLGEN
                     ILGTGFEFDLELRLGAGAYICGEETALFESIEGKRGFPRVKPPFPTTHGVFGKPTVIN
                     NVETLCNVPTILQHGAAAYRQMGTEKSPGPKLFCVSGDVARPGLYEVPFGITIRRLVY
                     DLAGGVRDGRSLQTILFGGAAGAFATPDQLDVRMSFEDLRAAGLPLGSGVVMVFDERR
                     DLRDVLVRLGRFFAHESCGKCYPCQLGTQRQYEILSRLAAGETRPGDAERLSDVGWTM
                     TDASLCGLGQTAATAVLSAMKIWPELFGN"
BASE COUNT         1074 a         1686 c         1649 g         1189 t
ORIGIN      
        1 ccagggatag tgctggtgcc gcgcaccagc accccatcca tatcgattag gtaattatta
       61 caggacatta tccatgactc cagtacaatc cagtgaataa caattaatat tgatgaatgc
      121 atctccccaa gccattatat aaatgacctg agcagagtta atcataccac cggctatatc
      181 gttcgctcga gtctgacgag gggcttgttc ttttctgccg attaaacgca aagagcacaa
      241 aggaagatgt cgagttcgtt ttattgctga ctttgtgccc tttggggtgt aaataacttg
      301 atcaggtgtt tttcagcggc aaaacaatgc gggtttgcgg ctcttcgtag ggtggttggc
      361 tggggtcgtt gaggtaatac tcgatggcca cgccggtggc ttcataaccc tgatctttga
      421 cgaattgggc caattcctcg taagcgggcc cgcaatctgc gtagggaccg acatataggt
      481 actccgcgac tttacccccc tccatgcggg cagcgtggat ctgacccttg cccggcagcg
      541 gctgggatac ggggaagccc agctcgatgt ctaaatcttg catgtccatg ttgtaataga
      601 ttacgaatgg atgtccggtt gggctcatcc ccagctcgcc caggtattgc atgatagctc
      661 catagccatc tcccagcacc tgtggtagat tctgcaccgc agccctggtg cggatgctca
      721 gtgttggctg agcaggctgc tccgtgattt cgcacgggtg agacattgca ctccctccca
      781 atagatgtaa agattgaacg aaaatctttt gtgaataata gcacatgcgt tctattttgt
      841 caagattggt gcggctgccg tccccacggc actggaatga tggctacaaa acctctatta
      901 atctcgatcc gtacgcctga tgatttggcg cgttgcctta atacctcccc cgccgtagca
      961 cagcctccgg gtgcggcagg tccgcgtcgc tgaccggctc gatcttcacc gcgcaggctt
     1021 tgaattccgg gatcttggcc tgcgggtcga gggcgtcaat ggtcagcagg ttggcggcag
     1081 cctcggcaaa gtggaagggg ataaagacca ccccggcgcg ggtcttctcg gtcaccgtgg
     1141 cgcgcagcgc caccgtcccg cgccgcgaag agacgcgcac cgcttgaccg tcgcgaatat
     1201 cgatcagggc tgcatcggta gggtgcaatt ccacccgcgc ctccgggtaa agatcgtcca
     1261 gcttagaatg gcgggtgagc gtcccgccgt gccagtgctc cagcacccgc ccggtgctca
     1321 ggatgaaggg gtactcgtcg tcaggtagct ccacgctggg cacaaactcc agcgcgtgga
     1381 acttgccccg cccgcgtgga aagctctggc tgaacaggta aggtgtgccc ggatggcttt
     1441 catcccatac cggtgtctgc aagccactct cttctaagcg ctcgtaggtc acgccggtaa
     1501 attccggcac cacgcggccc acctcggcca gcacctcggg aggctgggca taatcccagc
     1561 ctgtggaatt tgggcgcccc aggcgagcct cgatgcgctt ggccaggtcg cagatgatct
     1621 gccagtcggg gcgcgaatct ccgcgtgggg cgattgcctg gcgcacgcgc tgcacccgcc
     1681 ggtcagtgtt ggtgaaggtg ccgtcttttt ctgcccagga agtggatggc aggatcacat
     1741 cggcaaacgc tccggattcg ttgataaaca ggtcctgcgc cacgaggaac tcgagctgtt
     1801 ccatgtggtg gcgggtcagg ttcaggttgg gctcgctcat catcgggttt tcacccataa
     1861 tgtagagcga tcgcactccg ccagggcgag cgtgggagag gatttcggta gtggtcagcc
     1921 cgcgtttcag gctcaacccg ccgggttcga tattccaggc ccgctcccat ttggccgcat
     1981 tgtcgggatt atctaccagc atgtagcccg ggtaatggta aggcatggag cccatgtctg
     2041 acgcgccctg cacgttgttc tgtccccgta agggattcag cccggtgccc tctcgcccga
     2101 tgtgcccggt gagcagcgcc aggtggatga ctgccagggc gctggcagtg ccatgcgaga
     2161 gctgcgaaat gcccatgccc cagtaaatag cccctcgctc ggcgctggcg tacatgtgcg
     2221 ctgcttgcac gatcaggtcg cgctttaccc ctgaaaccat ctcggcatac tctggggtga
     2281 acttctccaa ggagcgggcg aactcgtcga agccctcggt gcgctgctcc acgaactggc
     2341 ggttgaccag gccatctcgg ataatgacgt gcgccatggc gctaaatacg ggcacgttcg
     2401 tgcctggctt gagcggcagc cacagggtgg caaagtcgca cagctctgag cgccgcgggt
     2461 cgatcacgat cagcttggcg ccgtgcttca tcacggctgc tttcatctgc agtgcgatga
     2521 tggggtggtt ctcgggtgtg ttcgagccgg tgacgatgaa tacatcgctc ttgatcacct
     2581 cggaggcggt gttgctcatg gctgacgatc cgactgcctg ctgcagcgcc accaccgagc
     2641 cagcatggca caggcgcgtg cagtggtcta cgttattggt gcggaacagg gagcgaaaca
     2701 tcttctgtag caggtagtta tcctcgttgg tggccttggc gcagcaatat actgccaggg
     2761 cgtctggccc atcgcggcga tagatttcta ccagccggtc agctacgtaa tccagcgctt
     2821 catcccagga gacctggcgc cactcatccc gttcgaaggc ttgcgtgcgc tttccaggct
     2881 cctggcgcac cttgcggagc agtggggttg tcactcgccg gcggtgatag acaaaatcgt
     2941 agccaaagcg ccctttcacg cacaggttcc cctggttcac tactgcgtca aagggtgagg
     3001 tcacccgata aattgtttcg tccttgacgt gcaattccag gttacatcca accccgcaat
     3061 aagggcaggt ggtcgatact actcggtcag gcttgatcat tgttgcctcc aaaaagagaa
     3121 tcgggattcg agcatcgaga ttggggattc gtaatctgcg tttctcgatt ctctactctt
     3181 gattctctac tctcgaatct cgcttccccc tgcgttcggt tcgcgtcatc tgcatgacct
     3241 catctggggt catcccctgc tcgagcagcc attcacgctt gggcttcaat gcaccagtgg
     3301 ggcacacgcc cacgcactgt ccgcagaaca cacaggtggt atcaggcatg gttttatcga
     3361 aaaatgtgcc gatggaggtc tcgaagccac gcccgctgaa gttgatcgcg taggtgtact
     3421 gggcgtcatc ggcgcatacc tgtacacagc gccagcacaa cacgcacttg gcgtagtcac
     3481 gcatgtacat gggattatcg tccaacaggg gtggctggcg tcgctcgctc cctgggaagg
     3541 gctcgtcatg aacgtcatac tctcccatga agtccagaat ctccggcgcc tctgacaggt
     3601 ccaccgccga ggccagcatc tccaggatgg tgcgccggct gcgctccacc ttggggctgc
     3661 gcgtctctac tttcatgccc tcgcttacct gtgctacgca ggaagggctg agcacgcgcg
     3721 cgccttctac ctctaccacg cacaagcggc acagggcgtt tgccgtgcaa tgatcgtgat
     3781 agcagatcgt gggtatcttt tcgccgattt gctcggcggc tttgaggatc gtggtccctg
     3841 ccgggacagt taccgaagag ccgtcgatgg taagcgttac tttctctggc atgcgagctc
     3901 cttcgaataa tatgggtaat atccgcgaat tacgcgaata tcgctaagca tttgtgtaat
     3961 tcgcgcagcg cggcatctct tcttaatttc caaatagttc aggccagatc ttcatggcac
     4021 tcagcaccgc cgtggcagca gtttgtccca agccgcacag agaagcgtcc gtcatggtcc
     4081 agcccacatc agacaggcgc tcggcatcgc caggccgcgt ctcacctgct gccagccggg
     4141 agaggatctc gtactgccgt tgcgtgccaa gctggcaggg gtaacacttg ccacacgatt
     4201 cgtgggcgaa gaatcgtccc aaccgtacca gcacatcgcg caggtcgcgc cgctcgtcga
     4261 agaccatcac tacaccagaa cccagcggca agcccgcggc gcgcaaatct tcgaagctca
     4321 tgcgaacgtc taattggtca ggcgtggcga aggcccctgc ggcgccacca aacaggatgg
     4381 tctgcagcga tcgcccgtcg cgtacgcctc ctgccaggtc gtacaccagg cggcggatgg
     4441 tgatgccgaa tggcacctcg tacagcccgg gacgagccac gtcgcctgag acacagaaca
     4501 gcttaggtcc tggagatttc tccgtaccca tctggcggta ggctgctgcc ccgtgctgaa
     4561 ggatggttgg cacgttacac agcgtctcta cattgttaat gacggtgggt tttccaaaca
     4621 ccccgtgggt ggtggggaag ggcggcttga cgcgcgggaa accgcgcttg ccctcgatag
     4681 actcgaacag ggcggtctcc tcaccgcaga tataagcccc cgcgccaagg cgcagctcga
     4741 ggtcgaactc gaagcccgtt cccaggatat tttcccccag gtagcccgcc tggcgcgcct
     4801 gggaaagggc gtcttccatg atctggtatg catgggggta ctcgccacgg atgtagatat
     4861 atccccggct tgctccagtg gcgtaagcgg cgatgagcat gccttcgagg atgagctgcg
     4921 ggtcaccctc cagcaggagg cgatctttga acgtcccagg ctcagattca tcggcgttgc
     4981 aaatcacata ctttggcgag ccttcggcct tggcggcgcc ctcccactta atcccggtgg
     5041 ggaaggctgc cccgccgcgc cccaccaacc cggaggcctt tacttcggcg atcacgctgt
     5101 cggggggcat gcgcaaagct ttcttcagcc cggcgaatcc ccctcgggcg gcgtactctt
     5161 gcaaggatgt cggctgcccg ttatcgcagt tggcggtcag gaggcgcagg tcaccaccca
     5221 gcacggcttg gagtggtgaa acatgacccg acagcaggtc gtcgagctgc agcgagccgg
     5281 cgtccccgac cgcccgatcg cccaccaggg ctgctggcgc gtgctcgcaa agccccaggc
     5341 aaggagcgcg ctccacctgg aatgccccgt cggggctggg ttcgtctgga ctgacgttga
     5401 gctggcgaca caggcgctcc agcacatcct cgccaccccc cagggcacag gctggatcgg
     5461 tgcacacgcg aaccatcgtc ttgccggtag gatggctgta gaacatgctg taaaagtcga
     5521 tcacgccgta cacatcggct aagggcacac ccagggcgcg gcctacctcg gcggcgaccg
     5581 gctcgggcag gtgcccgt
//
LOCUS       DBNGSX010000111         2868 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_146742, whole genome shotgun sequence.
ACCESSION   DBNGSX010000111 DBNGSX010000000
VERSION     DBNGSX010000111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_146742"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..157
                     /locus_tag="AC16CB_03745"
     CDS             59..157
                     /locus_tag="AC16CB_03745"
                     /inference="COORDINATES: protein motif:HMM:NF014628.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HIR3341615.1"
                     /translation="MIISGPSRTADIAMQLTLGMHGPRELQIVILS"
     gene            complement(390..647)
                     /locus_tag="AC16CB_03750"
     CDS             complement(390..647)
                     /locus_tag="AC16CB_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341616.1"
                     /translation="MMVQVIGADVAAKQVVWVKARPDFDVLFQFLHNLQVDDRRRFWI
                     LYPVADEDICDIGNELGQIATEVKIALPVSHNALPGVEKYP"
     gene            772..1413
                     /locus_tag="AC16CB_03755"
     CDS             772..1413
                     /locus_tag="AC16CB_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341617.1"
                     /translation="MSQQISLKEAEQKVFRTRYNDGLWDIFMGCFFLMFGLALYLSPI
                     LGDFWSSVALLPFWALLFLGIWLIRKYVVTPRIGSVKFGPARKVKLMRFNVVMLIVNF
                     IALLLGIAAAMSFGRVPGQMFSVGLGMILLMGFSMAAYFLDFTRLYIYGLLVGFSPLL
                     GEWLWNQGLASHHGFPITFGVSAGIMIIVGLTVFVRLLRNNPVPTEGTPSEEA"
     gene            1415..1741
                     /locus_tag="AC16CB_03760"
     CDS             1415..1741
                     /locus_tag="AC16CB_03760"
                     /inference="COORDINATES: protein motif:HMM:NF024988.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3341618.1"
                     /translation="MADHQSGSELHPLADIDQVIHASARLMVLTYLYVVESADYVFLM
                     RLTGLTWGNLSTHLTKLEEAGYITINKEFKGKKPHSTIHLTRQGRSAFKEYKKSLQQV
                     LDDLPD"
     gene            2252..2434
                     /locus_tag="AC16CB_03765"
     CDS             2252..2434
                     /locus_tag="AC16CB_03765"
                     /inference="COORDINATES: protein motif:HMM:NF025156.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HIR3341619.1"
                     /translation="MGFGGKSTDIWSKKDKKTGRTQWDTLTDLASSGWELIDVTPISV
                     GGTTSEILYTFKRPVE"
BASE COUNT          789 a          644 c          711 g          724 t
ORIGIN      
        1 ctggatagcc accctacgca aaaagggatt ggatgccttt cagggggcag ccagcgcaat
       61 gatcatcagc ggccccagtc gcacagcaga cattgccatg cagttaacgc tgggtatgca
      121 cgggccgcgg gaattacaga tcgtcattct aagctgaatc cgcaagtcgt tattcacttt
      181 gatgtagcaa tcgctccaat aaagacgatc caaccttagc tgttcacgga cgtgtgggta
      241 ggggacccac cctctccgca gagcatagta ctttcagagc atcttcgccg gcagcaacgc
      301 ttggtgaacg actgtcaaag tgctggaggt atacgctgga gatcgctcag ttccagctgg
      361 gcgatctcac ttttagtgca ataccttcat taagggtact tctcaacgcc tggcaaggca
      421 ttgtgggaca ccgggagggc gattttgact tccgtcgcta tctgtcccaa ttcattccca
      481 atgtcacaaa tgtcctcatc tgccacagga tacaaaatcc agaaacgccg tcgatcgtct
      541 acctgcaagt tgtgtaagaa ttggaaaagc acgtcaaaat ccgggcgggc tttcacccac
      601 acgacttgct tggcagcaac atcagcgcca attacctgaa ccatcatgcg agcaagattc
      661 tcgattggct attgacattc gagtaaaaag ttgtatacta aagtcaaagt actttctata
      721 tcaaagtact tttaggatca ggaaatttag ttcttcacaa ggaggtcaat catgtctcaa
      781 cagataagtc taaaagaggc tgaacagaag gtgtttagaa ccagatacaa tgatggattg
      841 tgggatattt tcatgggatg ctttttcttg atgtttgggc ttgcccttta tctcagtcct
      901 atcctgggag atttttggag ttcggtggcg ctcctaccat tttgggcctt gcttttcctg
      961 ggtatatggc tgattagaaa gtatgtagtc accccccgga ttggcagcgt gaaattcggt
     1021 ccagcgcgta aagtcaaatt aatgagattc aatgtcgtta tgctcatcgt taatttcatc
     1081 gccctcctcc taggcattgc cgccgcgatg agtttcggga gagtccccgg acagatgttt
     1141 tccgtcggcc tcgggatgat cctcctgatg ggtttcagca tggcagctta ttttttggat
     1201 tttacccgcc tgtacattta tggtctgtta gtcgggtttt cgcctcttct cggtgaatgg
     1261 ttatggaacc aaggacttgc ctctcatcac ggcttcccaa tcacctttgg cgtctcggca
     1321 ggcattatga tcattgtcgg cttgaccgtc tttgtgcgtc ttttgcgcaa caatcccgtt
     1381 cccacagaag gaacaccatc ggaagaagca tgagatggct gatcaccaat ctggctcaga
     1441 actgcacccg ctagccgaca ttgaccaggt catccatgca tcggcgcggt tgatggtact
     1501 cacctacctc tatgtcgttg agagcgctga ctatgttttc ctgatgcgcc tgacaggact
     1561 cacttgggga aacctgtcca cccatttaac caaactagaa gaagcgggct acattacgat
     1621 caataaggaa ttcaaaggca agaaacccca ttccaccatc cacctcaccc gccaggggcg
     1681 atctgctttc aaggagtata agaaaagctt gcagcaagta ctcgatgact tgcctgacta
     1741 atgggtaact gactttctaa ttcaccttga aggagagcat accatgcgta ttgcacagat
     1801 agcgtgaagc cggttatctt caagctagaa ggcattgaag ggtaatgcga ggttgaggaa
     1861 ctagcacgaa gagagagcaa tttcactaca gatgttctta gcaaagctag attttcggga
     1921 acacagaggt tagcaaagtg tgattcggcg tgagtctaat aaatctctgg agataacaca
     1981 gctgcaccgg gatgtaatcg tgggcttggg caattgcatc ggttgtatcc cgaggtcgcc
     2041 gagtagtggc caccgcgcgc ctcagctcac ccagttagat tgatcagtac atttatagag
     2101 tgtaaaccaa attattttta cattttgaaa aaaaaccgcc agacgagtac aatggcaggt
     2161 caagttaatt ttcgcaggaa gttagttggg ctaagttaaa gaaaggagtt ctgagatgca
     2221 gaagtgggag ttgtatgtta tgccttgtgg tatgggtttt gggggcaagt caactgatat
     2281 atggagcaag aaggataaaa agacaggaag aacgcagtgg gacacgctca cagacttggc
     2341 atctagcggg tgggagctta ttgacgtgac tccaatttct gtaggaggaa ccacttcaga
     2401 aattctatat accttcaaaa gaccagtaga gtaaatagag tgtaaaccca attaggtcaa
     2461 catttttgtc aaagccgcca gacgagtaca atagcgatat gaaaaaacaa cagttatggg
     2521 aatttccatc aaaaagcctg tacttctcca actgttggaa gatacaggct tttggtcaaa
     2581 cgttataccc accgtggagt gggcacccac cctctccgca ggttatatga attcccgagg
     2641 cggtgtaagt gtagcgtgtc agaacccgtg agacataatt aggggaaaaa agaatggaga
     2701 cttcttagcc aggataccaa gtaagatagt gaaaaggaaa ccacaatggc taagaagaaa
     2761 gagattgtag aacaaaacac acggaatatc cgcaggaaaa cgcgcaagca gtattcgtct
     2821 gaggagaagg tccgcattgt cttagaaggt tttagagggg agagcaca
//
LOCUS       DBNGSX010000112        11641 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1462765, whole genome shotgun sequence.
ACCESSION   DBNGSX010000112 DBNGSX010000000
VERSION     DBNGSX010000112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11641)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11641)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11641
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1462765"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="AC16CB_03770"
     CDS             complement(<1..1033)
                     /locus_tag="AC16CB_03770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HIR3341620.1"
                     /translation="MSAQPAEKPSSPPLPSAYLERMQRLLGGEFEAFLQSYQQPPSTG
                     LRVNTLKISPQAFQANFPYPLSPIPWCPSGFFLKETPTSPGKHPYHAAGLYYLQDPSA
                     MIVAELLRPQAGELVLDIAAAPGGKSTHLASWMGGDGVLVANETHPQRVWELAENLER
                     WGARNTVILNETPARLAQSFGDIFDKVLLDAPCSGEGMFRKNEMSRLEWSPQRVASCA
                     LRQSAILRETAGLVRPGGTLVYATCTFAPEENEAVIAMFLKENPHFELVPALPVPNVA
                     PGQPKWANFDARKKFGDTYTAALKGAVRLWPHHGAGEGHFIAMLRRTDQTNRDKKKPW
                     QPPAPSAEAA"
     gene            complement(1121..2470)
                     /locus_tag="AC16CB_03775"
     CDS             complement(1121..2470)
                     /locus_tag="AC16CB_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3341621.1"
                     /translation="MFDLSNEEILPLAQQHTFWTWSAQGRVEPIPVTRAKGVHFWDAE
                     GKRYLDFNSMTMCVNIGHGEERVVEAIAEQARQLPYAAPNMATKPRALLGKLLSEITP
                     GDLDHFLFTLGGADANENAIKLARATTGKHKILARYRSYHGATAGAMAATGDPRRVTW
                     EPNLMTGVVHFLDPYRYRSTFHRTNPDISESEFTQDYLNHLEEIIIYEGADSIAAILL
                     ETVTGTNGVIIPPEGYLQGVRALCDRYGILLIADEVMSGFGRTGKWFAVDHWGVVPDL
                     MTMAKGLTSAYAPLGAVGMRPQIAAFFNERVFQSGLTFTAHPISLAAAIANIRVMQDD
                     HIVEHAAAMGPVLRRMLTDLGEQHPSVGDARSIGLFGILELVRDRRTKEPMAPYDGSS
                     AEMVALRKYCLEHGLFLYTHWHTVLIIPPLIITEEQLAEGLEILDKALEITDAAANP"
     gene            2710..3159
                     /locus_tag="AC16CB_03780"
     CDS             2710..3159
                     /locus_tag="AC16CB_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341622.1"
                     /translation="MYSILLAVHNVLRWVVVVLAILALVRAYRGWLGRRDWGSADRRA
                     GVFFTSFMDLQLLLGLLLYIFFSPITKAALADFGAAMSNAGARFFALEHFVYMFVAVV
                     FAHMGNAFSKKAAEPHAKHQRAAIWYTLTVLAILLGMPWMRPLFPGW"
     gene            3304..4197
                     /locus_tag="AC16CB_03785"
     CDS             3304..4197
                     /locus_tag="AC16CB_03785"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3341623.1"
                     /translation="MINRDRRIFPYLETLFAVIVWGASFIATKVALRDISPVTLVWLR
                     FGIGVVILGIVTAARRQFAWPARSELGYFALLGFLGIAFHQWLQSTGLLTAQATTTAW
                     IIASTPIFMALLGWSFLKENLGRLQVAGIALAAVGVLLVVSKGDFSSLAAGKFGTPGD
                     FLILISAPNWAVFSALSQRGLQRHPATRMMLYVMSFGWLFSTLLLFTGPGVLELGQLT
                     RDGWLAMIFLGVFVSGLAYIAWYDALQAIPSSRVGAFLYLEPLVAVGVAAVVLSEPLL
                     LSSLLGGGTILFGVWMVNRQG"
     gene            4568..4933
                     /locus_tag="AC16CB_03790"
     CDS             4568..4933
                     /locus_tag="AC16CB_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR3341624.1"
                     /translation="MKLYVGNLSYNSTEEDIRTLFAQAGTVVSVDLIKDRFTGSSKGF
                     AFVEMSSQSEGENAIRMFDGYSLDDREIKVNVARPREERSSGGFDNRRGGYGGGRSQG
                     GQGGRGGRDRRGGGGTRRY"
     gene            5037..5537
                     /locus_tag="AC16CB_03795"
     CDS             5037..5537
                     /locus_tag="AC16CB_03795"
                     /inference="COORDINATES: protein motif:HMM:NF044704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009303 - rRNA transcription [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HIR3341625.1"
                     /translation="MEFQVGDKVVHWSYGPGEIIQLDEKSISGQNALYYVVQIRDLTL
                     WVPVQGGDQSSLRLPTSTREFRRLFKILRSPGEKISDDRYERKTQLTERMREGTLESI
                     CGIIRDLTTYSYSKKLNDYDASLLERAKKFLLEEWKISLSVPLSDAQRELDILLEEGR
                     IVAADE"
     gene            5838..6146
                     /locus_tag="AC16CB_03800"
     CDS             5838..6146
                     /locus_tag="AC16CB_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR3341626.1"
                     /translation="MKTKLFVGNLTHATTEDELSRLFAQVGKVTSIELISLPHLDTSK
                     HIAFVDMDSRLEAEKAIRLLNGSDLHGRALKVNIARPREARPASGGWYTDSPSPSRRR
                     "
     gene            6398..6685
                     /locus_tag="AC16CB_03805"
     CDS             6398..6685
                     /locus_tag="AC16CB_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR3341627.1"
                     /translation="MDTKLYVGNLSYDTTEDTLRSLLSQAGTVESVDMITDRDTGRPK
                     GFAFVTMGSQVDAEKAITMFNEKIVDNRTIKVNIARPREERPRAGGYGYRR"
     gene            6913..9108
                     /locus_tag="AC16CB_03810"
     CDS             6913..9108
                     /locus_tag="AC16CB_03810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3341628.1"
                     /translation="MTSIGKSVHRIDAFGKVTGQTLFPGDINLPDQASMKILFANRPH
                     AIIRRIDTSQAEALPGVIAVFTAKDVPVNEYGLIMNDQPVLCGPGSSKPFVDRVRFVG
                     DQVALVVAETEAIAARGRDLIQVEYEDLPIVTHALEAMRDGAPLLHPDRDSNIFCHYR
                     IRKGDAESAFSQADVIVEGEYHTPAQEHAYLQPEAGLGYIDEAGRLTVQVAGQWTHED
                     QKQIAHALGLPQDQVRVVYPAIGGAFGGREDMSVQIVLALASWRLSQRGINRPVKIIW
                     SREESIIAHHKRHPYFIKTRWGATRQGKLVAAEVEVIADGGAYAYTSTKVLGNATLMC
                     TGPYEIPNVKVDSYAVYTNNLPNGAFRGFGGPQGAFAAEMQMNHLAEKLGMDPVEIRQ
                     RNILREGALLSVGTPLPKGVSMPQVVERCGLESARLKEAISTSKVQPSHLKRGFGFAC
                     AFKNVGFSFGFPEQCWATIELHGGAKIERAVVHHAGADVGQGAHTVFAQMAAEALDLP
                     MDKVDLVVSDTAFTDNSGSSSASRMTFMAGNAIKGAAELALEKWRAEERPAIATYQYR
                     PPSTTPYDPQTGKSEPNISYGYVAQAVTVEVDTETGHVRILDVVSVDDVGKALNPLQV
                     QGQIEGAVVQAAGYTILENFVQSGGYVQTQHLSTYLIPTVLDVPERVQSLILEYPDPR
                     GPWGARGMAEMPFLPLAPAIVAAVHDAIGVWFHEFPLIPERVLRGLGKI"
     gene            9158..>11641
                     /locus_tag="AC16CB_03815"
     CDS             9158..>11641
                     /locus_tag="AC16CB_03815"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HIR3341629.1"
                     /translation="MEIAATIRLLGELLGQVITTEESPAIFDLEERVRGYAKARRSGL
                     AGSPQRVQAANRLAGEIRALTSQSARPVAGAFKLYFDLVNLAEENFRANLLRQEEFAN
                     HPHPIHDSIGEAINVLKDSGVSHDEMGQLLDDLHIELVLTAHPTEAKRRTILSKLQRI
                     AMQIRRLERGDLPPRQVVSSRKAILEEITALWHTDPARTTRIKVTDEIRTTLYFVDEV
                     FWDVLPRIYADLDAALAQHYPGLKATHPWLSLASWVGGDRDGNPNVTHAVTAEALRLH
                     RGLAVERHRLTLQDTARRLSLSASRVPPTPELAAWFESRRPLPAHVSFLESRYSNEPY
                     RLAISLLADDLAEASKDDVTACLLSRNPTNNRLRSNDFIYPLKIIRQSIPELVADGLP
                     LTVQRHFEIFGLHAARLDIREDSSRLSEALGELLRALGIHAHFEHANQAERLVLLTEL
                     LASPKPDFAPQPGVTPRTAETFALFQLVNRTREVYGAELIGPVVISMARGAADVLAVL
                     LLARWAGCANCLDIVPLFETLDDLRAAPKVMSELFNLDVYRAHLATCRDHQMVMVGYS
                     DSNKDGGYLAANWALYRAQEDIAQVCKQHGVRFTLFHGRGGTVARGGGPANRAIRAQP
                     PATVQGRFRLTEQGEVISSRYAEPQLAYRHLEQIINAVLLSSFPDKMDHGRAVQEKWR
                     VAMNGMAVAAHRAYRRLVYETPGFIDYWLVVTPIEEIQRLQIGSRPAARQSEENSLAR
                     VRAIPWVFSWMQSRFNLPGWYGLGSGLAASPLALPEMKEMYAEWPFFRSLFDNAEMSL
                     LKADLDIAALYSALMPDQVFAKRIFDDIQR"
BASE COUNT         2503 a         3032 c         3281 g         2825 t
ORIGIN      
        1 gagcggcttc tgcagatggg gctggcggtt gccagggctt ctttttatct cggttggttt
       61 gatcagtcct acgaagcatg gcaataaaat gtccttctcc tgcgccgtga tggggccata
      121 agcgcactgc tccctttagc gcagccgtgt aggtgtctcc aaatttcttc cttgcgtcga
      181 aatttgccca cttgggctgt ccaggcgcca cgttgggcac cggcagcgcc ggcacgagct
      241 cgaagtgggg gttctccttt agaaacatag cgatgactgc ctcgttctct tcaggtgcaa
      301 acgtgcaagt ggcgtagacc agtgtgccgc cgggtcgcac cagcccagcc gtctctctca
      361 gaatagcact ctgccgcaag gcgcagcttg ccacccgctg tggcgaccat tccaggcggg
      421 acatctcatt cttgcggaac ataccctctc cagagcaggg tgcatccagc agcactttat
      481 cgaaaatgtc accaaaggat tgtgccagcc gcgcaggtgt ctcgttcaag atgacggtgt
      541 ttcgcgctcc ccagcgttct aagttctctg ccagctccca aacacgttgt gggtgtgtct
      601 cattcgccac caggacgccg tctccgccca tccaggatgc caggtgggta gacttgccgc
      661 ctggtgcagc ggcaatatct aatactagct cgcccgcttg aggtcgaagc agctcggcga
      721 cgatcattgc cgatgggtct tgcaggtaat acagcccggc agcgtgatag gggtgcttgc
      781 cgggtgatgt gggcgtttct ttaagaaaga atccagaggg gcaccagggg atgggggaga
      841 ggggataggg aaaattagcc tggaaggctt ggggtgagat tttcagggtg ttgacgcgca
      901 ggccagtgct gggcggctgt tggtagctct gcaagaaggc ctcgaattca ccgcccagga
      961 ggcgttgcat gcgctcaaga taggccgacg gcagcggtgg ggaggagggc ttttcagcag
     1021 gttgcgcgct catctttgac agcccgcgag acgacagttt atctctgccc ttcccgtgat
     1081 cctgttaata agtgatgtgg gtagcgatcc atttccggca tcagggattc gcggctgcgt
     1141 ctgtaatctc tagcgcctta tccaaaattt caagcccttc agccagctgc tcttccgtga
     1201 taatcagcgg agggatgatc agcacagtgt gccaatgggt atataagaat aagccatgct
     1261 cgaggcaata cttgcgcagg gcaaccatct ctgcgctgga gccgtcgtag ggagccatgg
     1321 gctccttggt gcgccggtcg cgcaccagct cgaggatgcc gaacaggccg atagagcggg
     1381 catcgcccac ggaagggtgc tgttcgccga gatcagtgag catgcggcgc agcaccggcc
     1441 ccatggcggc ggcgtgctca acgatatggt cgtcctgcat gacgcggata ttggcgatag
     1501 cagccgccag cgagataggg tgagctgtga aggtcagccc gctttgaaag acgcgctcgt
     1561 tgaaaaaggc ggcgatttgt gggcgcatcc ccaccgcacc cagcggggca taagccgagg
     1621 tcaatccctt ggccatagtc attaggtcgg ggacaacgcc ccagtgatcc acagcaaacc
     1681 acttgccggt gcgcccaaat ccactcatca cctcatccgc gatgagcagg atgccatagc
     1741 gatcgcataa tgctcgcact ccttgaagat aaccctctgg gggaatgatg actccgttag
     1801 ttccggtgac agtttccagc aggatagcag cgatgctgtc cgccccctca taaatgataa
     1861 tttcttctag gtgattgagg taatcctggg tgaactcgct ctcggagata tccgggtttg
     1921 tgcggtgaaa ggtggagcgg taacggtagg gatccaggaa gtgtaccacg ccggtcatca
     1981 ggttgggttc ccaggttacg cgccgcgggt cgccggtggc tgccatagct cccgctgtgg
     2041 ccccgtgata ggagcggtag cgggctagga ttttatgctt gcctgtggtt gcccgtgcca
     2101 gcttaatggc gttctcattg gcgtcggcgc cgcccagggt gaacaggaag tgatccaagt
     2161 cgccaggcgt gatctcgctc agcagtttgc ctaataatgc tcgtggcttg gtcgccatgt
     2221 tcggcgcagc gtaggggagc tggcgcgcct gctcggcgat ggcttcaacg acgcgctctt
     2281 cgccatgccc aatattgacg cacatggtca tggagttgaa atccaggtag cgcttgcctt
     2341 ccgcatccca aaagtgcacg cctttggcgc gtgtcaccgg gattggctca actcgccctt
     2401 gtgccgacca ggtccagaat gtgtgctgct gagccagggg gaggatctct tcattggaaa
     2461 ggtcgaacat ggttgcctcg tgattagaaa ggttctcaaa cacaagatga gggggtgcct
     2521 tgctgcgacg cagtaactgc aaaacacccc aaataaccat tttatgatga gttctagtct
     2581 tgatgtcaat ggtatcacag aaggcaatag tcaatcaacc tcgaaacctt ctcacgctag
     2641 gcaatttcgg ctaaactatt cgggtgcttg tctcgttttt ggaataatct gcaatggagg
     2701 tccctttcga tgtattctat actactcgct gttcataatg tattgcgctg ggttgtcgtc
     2761 gtgctggcta tcctggcttt ggttcgagcc taccggggct ggctgggcag gcgggattgg
     2821 ggcagcgctg accgccgcgc tggcgtgttc ttcacttcat ttatggatct tcagcttttg
     2881 cttgggttgt tgctctatat tttcttcagc ccgatcacga aggctgccct agctgatttt
     2941 ggggcggcca tgagtaatgc tggggcgcgt ttctttgccc tggagcattt cgtgtatatg
     3001 tttgtggcag tagtttttgc tcacatggga aatgccttct ccaagaaagc ggctgagcca
     3061 catgccaagc accagcgcgc cgccatctgg tacacgctga ctgttctggc tatcctgcta
     3121 ggcatgccct ggatgaggcc gctgttcccc ggttggtgat gccattcccc acccaaccct
     3181 catctctcca ctcttgcttc ccagtggtaa gctgggtaac cgccgaaatt aagctatgct
     3241 ccttatttta gcctctatat atttcctctt atgagatatg gggaatctat cgcacaactg
     3301 tatatgatca atcgagaccg taggatattc ccttatcttg aaacgctgtt tgcggtcatt
     3361 gtttgggggg cttctttcat tgccaccaag gttgccttgc gagatatctc gccagtgacc
     3421 ctggtttggc tgcgatttgg aattggggtg gttatcttgg gaattgttac ggctgcacgc
     3481 cgacaatttg cttggcctgc ccgcagcgag ctgggctact tcgccttgtt gggctttttg
     3541 ggcatcgctt tccatcaatg gctgcaatcc actggactgt taactgccca ggcgaccacc
     3601 accgcctgga tcattgcctc cacgccgata tttatggcgc tcttaggctg gtcctttctc
     3661 aaggaaaacc ttggcaggct acaggttgct ggaatcgctc tggctgcggt gggtgttttg
     3721 ctggtagtaa gcaaaggtga tttctctagc ctggccgcag gaaaatttgg cactccggga
     3781 gatttcctca tccttatcag cgcacccaac tgggcggtgt tctctgccct ttcgcagcgg
     3841 ggattgcagc ggcatccagc cacgcgcatg atgttgtacg tgatgtcctt tggctggtta
     3901 ttctctacct tgttattgtt tactggcccg ggcgtccttg agcttgggca attgacccga
     3961 gatggctggc tggcgatgat cttcctaggc gtgttcgtgt cggggttggc ctatatcgcc
     4021 tggtatgacg ctttgcaggc tatcccctct tcgcgggtcg gcgcatttct ttacctcgag
     4081 ccactcgtgg cggttggcgt ggcggcagtc gtgctgagtg agccgctgtt gctgtcctcg
     4141 ctcttgggag gagggacgat cttgtttggg gtgtggatgg tgaatcgcca gggttgatga
     4201 caccacaacc cccgtttcgc gaggttgcac atgcccagaa gggtgttgta aatgccctga
     4261 tttcaaaggt agggcaatcg cattagggtg atttcccacc gctgaagaga gtaatttggg
     4321 tgttttggtg gggggatacc ccaccaaaac acccaaaacc ctcgttttgc gggattgcgc
     4381 atgcccctca gggcatatgc aattgccctg atttcaaagg ggctttttac cggctggggt
     4441 cagaatatgg taaaatgtag ttgtccctgt caaaggattt gcgtgttgga taaccgccat
     4501 tcctgtttgt aaggatggcg gaattttatt gcctatctac tcaatagccc gaaaggatca
     4561 tttgaaaatg aagctctatg ttggaaatct gtcctataac tcaactgaag aggacatccg
     4621 tacattgttc gcccaggcgg gaacagtggt ctccgtggat ctgatcaagg atcgctttac
     4681 gggctcttcg aaaggcttcg catttgtgga gatgagctcc caaagcgaag gcgagaacgc
     4741 catccgtatg tttgatggat atagcttgga tgaccgcgag atcaaggtaa atgtggctcg
     4801 cccacgcgag gagcgcagtt ctggtggctt tgacaatcgg cgcggcggct acggcggcgg
     4861 tcgtagtcaa ggcggtcaag gtggtcgtgg cggtcgggac cgacgcggtg gcggtggtac
     4921 ccgcaggtat taaaatcaga acttgtttcg ttaaagaaaa aaattggggg ttgggcggca
     4981 ttgtccgcca accccttact ttctcgccaa ctgattagct tcttaaagta ggaattgtgg
     5041 aatttcaagt aggtgataaa gtagttcact ggtcgtatgg tccgggagag atcattcagc
     5101 tggacgagaa gagcatctcg ggccagaatg ctttgtatta tgtggtccag atcagggacc
     5161 tcaccctatg ggttccagtg cagggaggcg atcaatctag ccttcgattg ccaacctcga
     5221 cgcgtgagtt taggagactt tttaaaatat tacgcagtcc tggagagaaa atttctgacg
     5281 atcgctatga gcgaaaaact caattgactg agcgcatgcg agaagggacg cttgagtcga
     5341 tctgcggcat tattcgtgat ctcactacct atagctattc aaagaaacta aatgactatg
     5401 atgcatccct gctggagcgt gctaaaaaat tcttgctgga ggagtggaag atttccctct
     5461 ctgtcccatt aagtgatgct caacgcgaat tggatatact tttagaagag gggcgaatcg
     5521 tcgctgcgga tgaatgaagt gctgcgagcg catcacttga ttgtatcagc aaaacaataa
     5581 agacagatca gaatcaccca attggaaatc gcctcacgct tgggctggtg gtatcgccgc
     5641 gaagaggagg gaactccggt cattaagtgt cagagagtgc cataccacca tcatcttatt
     5701 atctaagaat aatcttcatc taatcttggc gtgttagaat gctttaatca ttcttcgatt
     5761 ggtacctgcc ctaacacaat gaaggtgata cctatcagta aacagggatt gtaaatccta
     5821 taagtatcga ggtaaatatg aaaaccaaat tgttcgttgg aaatctgact catgctacaa
     5881 ccgaagatga actgagcaga ctctttgccc aggtgggtaa ggtgacttcc attgaactga
     5941 tttctctccc gcatttagac acatcaaaac acattgcctt tgtggatatg gatagccgcc
     6001 ttgaggccga gaaagccatc cggctactga atggatctga tctgcacggt cgagccttga
     6061 aggttaatat cgctcgccca cgtgaggcgc gcccggcaag tgggggctgg tacacagact
     6121 ccccgtctcc cagccgccgg cgctaaaacc catttcgcaa caagtaatta gctcaggact
     6181 tgcgcagttc aaagacatcc taactacgta atttcaatag atgagtaaat ggttagaata
     6241 tccccattta caagtcttga gtgctttatg tggtagaata cttgagtctt ataatcagat
     6301 ttgcgtgtat tgatgtgtcg ccattccgct ttataaggga atggcggtgt ttgttaacca
     6361 cggatctgca caattaaatt taaggagaaa gttagaaatg gatacgaaat tgtacgttgg
     6421 aaatttgtct tatgatacga ccgaagatac cttgcgcagc ctgctgagcc aggcgggcac
     6481 agtggaatcc gtggatatga tcacagatcg ggatactggg agaccgaaag gttttgcctt
     6541 tgtcaccatg ggcagccagg ttgatgcaga gaaagcgatt accatgttca acgagaagat
     6601 cgtcgataat cgcacgatca aggtcaatat cgcccgtccc cgcgaagagc ggccgcgcgc
     6661 gggtggttac ggctaccgcc ggtaatcatt ccacatgtca tagggacccc acccaattac
     6721 ggtggggttt tttattttaa gtacttccac agttgaacaa atggactggg gatctatcct
     6781 gatccactgt acccccacac aggacttctc aactcgctcg gcgtactatg actgatcgct
     6841 cgcggggtat aatacacaca tctcacctag agatataacg tttgttattc caaccatctc
     6901 atcggagtac ccatgacaag cattggaaaa tccgtccacc gcatcgatgc gttcggcaaa
     6961 gtcactgggc agacactctt tcctggtgat atcaacttgc cagatcaggc aagtatgaag
     7021 attctgttcg ccaaccgtcc tcacgccatc atccgccgta tcgataccag ccaggccgaa
     7081 gccctgccgg gcgtaatcgc cgtcttcacc gccaaggatg tacctgttaa cgagtacggg
     7141 ctgatcatga atgaccagcc agtgctgtgc ggtcctggtt ccagcaaacc ttttgttgac
     7201 cgtgtgcgtt tcgtgggtga ccaggtagca ctggtggttg ccgagaccga agccattgct
     7261 gcccgggggc gcgacctgat ccaagtggag tatgaggatc tacctattgt cacccatgcg
     7321 ctggaagcca tgcgagatgg cgcgcctcta ttgcaccccg accgagactc caatatcttc
     7381 tgccattacc gcatccgcaa gggagatgcg gagtctgcct tctcacaagc cgatgtgatt
     7441 gttgaaggcg agtatcacac acctgcccag gaacatgctt acctgcaacc cgaggctggg
     7501 ctgggctaca ttgacgaagc cgggcggctc actgtccagg tcgcaggaca gtggacgcat
     7561 gaagaccaga agcagatcgc ccacgctctt ggactaccgc aagatcaggt gcgcgtggta
     7621 tatccggcga taggcggtgc atttggcggg cgcgaagata tgtccgtgca gattgtcctg
     7681 gcactagcat cctggcggtt aagccagcgc ggcatcaacc gtccagtaaa gatcatctgg
     7741 agtcgcgaag aatcgattat tgcgcaccat aagcgccatc cttacttcat aaaaacccgt
     7801 tggggtgcca ctcgccaggg taaactagtc gcagccgaag tggaagtcat tgcagatggt
     7861 ggtgcctacg cctacacttc caccaaggtg ctgggcaatg ccaccttgat gtgtactgga
     7921 ccgtacgaga tccccaatgt taaagtggat tcgtatgcgg tctacaccaa caacttgcct
     7981 aacggtgcgt ttcgcggctt cgggggaccc cagggcgcgt ttgccgctga gatgcagatg
     8041 aaccacctag ccgaaaagct aggcatggac ccggtggaga tccgccaacg caacattttg
     8101 cgcgagggtg cgctgctctc tgtgggcaca cctttgccca aaggggtcag catgccgcag
     8161 gttgtcgaac gttgcgggtt agagagcgcc aggttaaagg aggcgatcag cacatccaag
     8221 gtacagccat ctcacctgaa gcgcggtttc ggttttgcct gcgcctttaa gaatgtgggc
     8281 ttctccttcg gctttccaga gcagtgctgg gcgaccatcg agcttcacgg cggcgcaaag
     8341 atcgagcgcg ctgttgtcca ccatgcgggg gctgatgtag ggcagggtgc gcacaccgtc
     8401 ttcgcacaga tggcggcgga agcccttgac ctgccgatgg acaaggtcga tctggtcgtt
     8461 tccgacacgg cttttacgga taactccggc agttcgtcgg cctcgcgcat gactttcatg
     8521 gcgggcaatg cgattaaggg cgctgccgag ttggccctgg agaaatggcg cgcagaggaa
     8581 cgtccggcaa ttgcgacata ccaataccgc cctccaagca caacgcccta tgatccccag
     8641 actggcaagt cggagccaaa tatcagctac ggatacgtcg cccaggcggt aaccgtggag
     8701 gtggataccg agactgggca tgtgcgcatc ctagatgtgg tttcggttga tgatgtgggc
     8761 aaagcgttga atcccctgca ggtgcaggga cagatcgagg gggcggtcgt acaggctgcc
     8821 ggatacacca tcctggagaa ctttgttcaa agcggaggct acgtacagac ccagcatctc
     8881 tctacctatt tgattcccac agtactggat gtgcccgaac gggtgcaatc tttgattctg
     8941 gaatacccag acccgcgcgg accttggggg gcgcgcggta tggcagagat gcctttcctg
     9001 ccgctggcgc ctgcgatcgt tgccgcggtt cacgacgcga tcggcgtgtg gttccacgaa
     9061 ttcccgctca tccccgagcg cgttttgcgc ggtttaggca aaatctgaca ttgactggat
     9121 tgcaacggtc attgcaagta ctccccactg gtccggcatg gaaatagcgg caacaattcg
     9181 tttacttggt gaacttctcg gtcaggttat cactacagaa gaatccccgg cgatcttcga
     9241 tctagaagag cgcgtccgtg gatatgccaa agcccggcga agtggactcg ctggcagtcc
     9301 gcaacgcgtc caagccgcca ataggttggc aggagaaatt cgtgccctga ccagccagtc
     9361 tgctcgtccc gtggcaggcg ccttcaagct gtactttgac ctggtcaatc tcgctgaaga
     9421 aaattttcga gccaacctgc tgcgccagga agagttcgcc aatcacccgc accccattca
     9481 cgattcgatc ggcgaagcaa tcaacgtatt gaaggattct ggggtttctc acgatgagat
     9541 gggacaactt ttagatgatc tgcacatcga gctggtcctc acagctcatc ctaccgaagc
     9601 gaaacgtcgc accattcttt ccaagttgca aaggattgcc atgcagatac gccgcctgga
     9661 gcgcggtgac ctgccgccgc gccaagtagt ttcatcccgc aaagccatat tggaggagat
     9721 taccgcactc tggcatacag accccgcccg cacaactcgg atcaaagtca ccgatgagat
     9781 acgtacaacc ctatacttcg tagatgaagt attttgggac gtgttaccgc gcatatatgc
     9841 ggacttagac gctgcacttg cgcagcacta tcctggttta aaagccacgc acccttggct
     9901 gagcctggct tcctgggtgg gaggtgaccg tgacggcaac ccgaatgtga cccatgctgt
     9961 tacggctgag gcgctaagat tgcaccgtgg gctggcagta gagcggcatc ggcttacatt
    10021 gcaagatacg gcgcggcgtt tgagtttgag cgccagccgg gtgccgccaa caccagagct
    10081 ggctgcctgg tttgagagcc gcagaccgtt gccggcgcat gtgtcctttt tagagagccg
    10141 ttattcgaat gagccctatc gcctagcgat ttccttgctt gccgatgatc tggcagaagc
    10201 ctccaaggat gatgtgactg cttgcctctt gagccgcaac ccgacaaaca accgccttcg
    10261 ctcaaatgat tttatatatc ctttgaaaat tattcgccaa tctattccag agctggtcgc
    10321 cgatggtttg ccgctcacag ttcaacggca cttcgaaatc tttggcttgc acgcagcacg
    10381 tttggatatc cgcgaagact cttcccgttt gagcgaagcg ctaggcgaac tattgcgcgc
    10441 gctggggatt catgctcatt tcgagcatgc taatcaggct gagcgcctgg ttttacttac
    10501 ggaattgctg gcatctccca aacctgactt cgcccctcaa cctggtgtga cccctcgaac
    10561 agcggaaacc tttgccctgt ttcaattggt gaaccgcacc cgtgaagtat atggggctga
    10621 gctcatcgga ccggttgtca tttcgatggc ccgtggtgct gcagatgttc tcgcggtact
    10681 gcttctggcg cgctgggcgg gttgcgcaaa ttgccttgat attgtcccat tgtttgaaac
    10741 cctggacgat ctgcgtgccg cgcccaaagt catgtcagag ctgttcaatt tagatgtata
    10801 ccgggcccat ctggcgacct gccgtgacca tcaaatggtt atggtaggtt attccgatag
    10861 caataaggat ggcggttatc tggcagcgaa ttgggccttg tatcgggcgc aggaagacat
    10921 cgctcaggtt tgcaaacagc atggggtgcg ttttaccctg tttcacggtc gtggtgggac
    10981 cgttgcacgg ggaggtgggc ctgctaaccg ggcaatacgc gcccaaccac ccgctacagt
    11041 gcaagggcgc tttcgtctga cagaacaagg cgaggtgatt tcctctcgct acgctgaacc
    11101 acagttggca tatcgtcact tggagcagat tatcaacgcg gtgctgctat cttcatttcc
    11161 agataagatg gatcatggtc gtgctgtgca agaaaaatgg cgggtcgcaa tgaatggcat
    11221 ggctgtggcg gcgcaccgtg cttaccgccg attggtttat gagacgcccg gttttataga
    11281 ctactggctt gttgtaaccc ccatcgagga gattcagcgc cttcagatag gctcccggcc
    11341 ggctgcccgt caatctgagg aaaattctct tgcgcgcgtc cgtgccattc cctgggtgtt
    11401 ctcctggatg cagagtcgtt tcaatctgcc gggctggtac gggctgggat ccgggttggc
    11461 tgcttcaccc ctggcgcttc cagagatgaa agagatgtat gcggagtggc ccttctttcg
    11521 cagcctgttc gacaacgctg aaatgtcctt gttaaaagcg gacctcgaca tcgctgccct
    11581 ttactcggct ttaatgccgg atcaagtctt tgctaagagg attttcgatg atatccagag
    11641 g
//
LOCUS       DBNGSX010000113         4345 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_147062, whole genome shotgun sequence.
ACCESSION   DBNGSX010000113 DBNGSX010000000
VERSION     DBNGSX010000113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_147062"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="AC16CB_03820"
     CDS             complement(<1..329)
                     /locus_tag="AC16CB_03820"
                     /inference="COORDINATES: protein motif:HMM:NF013112.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HIR3341630.1"
                     /translation="MAQLLVGSGYRLQGDAANAEVVIVNTCGFIGPAKEESLQVLSDL
                     AAHKRRGQLLIAAGCLTQRYGVEVARQVPGIDGILGTRRWMDIVDVVTRLRDGAHPEP
                     IYHLPDAP"
     gene            complement(695..1948)
                     /locus_tag="AC16CB_03825"
     CDS             complement(695..1948)
                     /locus_tag="AC16CB_03825"
                     /inference="COORDINATES: protein motif:HMM:NF024805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HIR3341631.1"
                     /translation="MSESIGQQLQQARQARSLTLEQVARATHLRVHYLQALEAGDLSR
                     VPSLVQARGFLRIYAEYLGLNVKTLLGELEGLASEPPVAPAPPEAQAEPVSAGSAEPL
                     ASPAEATSAFTEIGLKLRQQREVLGLSLDDVERHTHLRIFYLKALEAGELDRLPSPVQ
                     GRGMLSNYAEFLGMNPEPLLLRFADGLQAKLVERQAIKQPPRPRAERRPPRPPSLLRR
                     LFSAELLVGLSLVVVLVGFALWAAIRINALREEQQPAPTAPPIAAVLLATPVESPSPS
                     PVPPTATIPAPVAALAQPTEGTAQEALPPLASGEFVQVYVTVRQRTWMRVLVDGEVEF
                     EGRVIPGSAYQYSGEEKVEIVTGNGAALQVFYNQNDLGIIGLYGEVVHQVYTLQGIQS
                     PTPTMTMTPTTTARPSRTPRVTATP"
     gene            complement(2059..3063)
                     /gene="rplI"
                     /locus_tag="AC16CB_03830"
     CDS             complement(2059..3063)
                     /gene="rplI"
                     /locus_tag="AC16CB_03830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR3341632.1"
                     /translation="MKVLLLEDVYKLGRAGDVKRVADGYGRNYLIPQGLAVLATPGAL
                     KQVEHIRARAETSRAALNQEMSGIAERLAKLTLAFPARAGETGKLYGSITSQMIADSI
                     QDKLGLEIDRRQLDAQPLRTLGEHNIHVRLTIDLVPEVRVIVYREGETVDLGEEEVAA
                     EAVAEAEVAEESAPEAEVAEVSAPEAEVAEVSEPEAEVAEVSEPEAEVAEVSEPEAEE
                     AEVPEPEAEVIEEAQPEAEVAEVPEPEAEVTEEAQPEAEVAEVPEPEAEVAEVPEPEA
                     EVAEESEPEAEEAEESEPEAEVAEEAQPEAEVAEEAQPEAEEAEEAQPDTEAPAKAAE
                     "
     gene            3221..4018
                     /gene="recO"
                     /locus_tag="AC16CB_03835"
     CDS             3221..4018
                     /gene="recO"
                     /locus_tag="AC16CB_03835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR3341633.1"
                     /translation="MPSRDRSFRVEAVVLRHSDWGEADRLLWLFTREMGKVKAVAKGV
                     RKLRSRKAGHLEPQTRVNLLLARGRDILLVTQAETVEAFLPLREDLKLWEYACHIIEL
                     LDRFTYEEDENRALYRLLVDTLGRLARQPQAELVVHYYEIRLLDLLGYRPHLFHCANC
                     SKEIQPEDQYFSADQGGVLCPSCGREVKGARPITMSALKYLRHFQRSSFSEAARARIP
                     HNVMREIEALMEYYLTYLLEQGLNSLAFIRRMRRGEMSNKARDQEII"
     gene            4116..>4345
                     /locus_tag="AC16CB_03840"
     CDS             4116..>4345
                     /locus_tag="AC16CB_03840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013426.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE family
                     protein"
                     /protein_id="HIR3341634.1"
                     /translation="MSDFDLAPLQPVLAEFTTQGRTALLPALHAAQKLYGHLPEPVAA
                     EVGRALGVPLADVYGVIDFYSMFYSHPTGKTMV"
BASE COUNT          895 a         1271 c         1194 g          985 t
ORIGIN      
        1 ggtgcatctg gcagatggta aatgggctcg ggatgggcac cgtctcgcaa gcgggtcacc
       61 acatccacga tatccatcca gcgacgggtc cccaggatgc cgtcaatgcc aggcacttgc
      121 cgggcaacct cgacgccgta acgttgcgtc aggcatccgg ctgcaataag cagctggcct
      181 cggcgcttgt gcgcggccag atcgctcaaa acctgcagcg attcctcttt cgccgggccg
      241 ataaaaccac aggtgtttac aatcaccact tctgcattgg cggcatcacc ttgcaggcgg
      301 taacctgatc caaccagtaa ttgcgccatc gactgcgaat caacggtgtt tttcgcacac
      361 cctaacgata ccaagtgaaa tgttttcgat ttcttactag agctcatcaa taagttgatt
      421 ccttgttctt aatatcttac cttcggataa cagttcggtc atttctcgaa atcctgtcat
      481 cgcgagcctg ccgcaatttg gcaggcgaag cgatctcata gaaatgtcag gagattgcca
      541 cgcccctgcg gggctcgcaa tgactccttt agtggatatt gtccgaactg ttctgaaaat
      601 agccattttc atgtttagtg gtgaacacag ttcatgaaga cttttgtctt ttgtctcaac
      661 ctcagcttaa tatatcttga aagcgtaccc tcgctcatgg agtagctgtg actctggggg
      721 tgcggctggg acgtgccgtg gtggtgggcg tcatcgtcat ggtaggtgtt ggggattgta
      781 tgccctgcag agtgtagacc tggtgaacca cctcgccata caggccaatg atcccgaggt
      841 cattttgatt ataaaacacc tgtaacgcag ctccattgcc tgtcactatc tcaacctttt
      901 cctcgccaga atactggtag gcgctgcccg gaataacacg cccttcaaac tccacctctc
      961 catccaccag cacgcgcatc caggtgcgct ggcgtacagt gacatagacc tggacaaact
     1021 ccccgctggc aaggggtggc agggcctcct gggcggtgcc ttcggttggc tgtgccaagg
     1081 cagccacagg tgccgggata gttgccgtgg gcggcacagg cgatggtgaa ggggattcaa
     1141 caggcgtggc cagcaggacg gctgcaatgg ggggagcagt aggagcaggc tgctgctctt
     1201 cccgcagggc gttaatgcga atggcagccc acagggcaaa tccgactagc actaccacca
     1261 gggataggcc aaccagcaac tcggcggaaa ataagcgccg cagcaaggac ggtgggcggg
     1321 gagggcgacg ttcggcgcga gggcgcgggg gctgctttat cgcctgtcgc tcaaccagct
     1381 tagcctgcaa gccatcagca aagcgcaaaa gcagaggctc aggattcatc cccagaaatt
     1441 cagcatagtt gctgagcatg ccgcgtccct gcaccggcga gggcaagcga tctaattcac
     1501 ccgcctcgag cgcctttaga taaaagatac gcaggtgggt gtgtcgctcg acgtcgtcta
     1561 aagacaggcc cagcacctcg cgctgctggc gcagcttcag accaatctca gtgaaggccg
     1621 aggttgcctc agccgggctg gcgagtggct cggcagaacc agctgagaca ggctcagcct
     1681 gagcttcagg tggagcaggg gcaactgggg gctcagaggc cagcccctcc agctcgccca
     1741 gcaatgtctt aacattcaac ccaaggtatt cggcataaat acgcaggaaa ccgcgcgcct
     1801 gcaccaaaga gggcactctg gagagatcac cggcttccaa tgcttgcaag taatggacgc
     1861 gcaggtgcgt cgctctggcg acttgctcca aggtgagcga tcgcgcctgg cgtgcttgct
     1921 ggagctgttg accgatcgac tctgacatta cggttaccag gtatgattac ggcaacaggg
     1981 gcggggtttc cagacgctgg ttggttaccc cgcccctgtg gatgctacac aggagcgctg
     2041 tttcctgccc ggacggaact actcagcagc ttttgcaggc gcctctgtat caggctgagc
     2101 ttcctcagcc tcttcggctt ctggctgagc ttcttcggcc acttcagctt ctggctgagc
     2161 ttcttcggcc acttcggctt ctggctctga ctcttcagcc tcttcggctt ctggctctga
     2221 ctcttcagcc acttccgcct ctggctctgg cacttcagcc acttcagcct ctggctctgg
     2281 cacttcagcc acttcagcct ctggctgagc ttcttcggtc acttctgcct ctggctctgg
     2341 cacttcagcc acttctgcct ctggctgagc ttcttcgatc acttccgctt ctggctctgg
     2401 cacttcagcc tcttcggctt ctggctctga aacttcggcc acttctgcct ctggctctga
     2461 cacttcagcc acttctgcct ctggctctga aacttcggcc acttctgctt ctggcgctga
     2521 cacttcagcc acttctgctt ctggcgctga ctcttcagct acttcagcct cagccactgc
     2581 ttcagcggca acttcctcct cgcccagatc gaccgtctct ccctcacggt agacgatcac
     2641 ccgcacttcg gggaccagat cgatagtgag gcgcacatga atattatgtt cgcccagcgt
     2701 gcgcaggggc tgtgcgtcca gctggcggcg atcgatttcc aggcccagct tatcctgaat
     2761 agagtcggca atcatctggg aagtgatgga accgtacaat tttccggtct ctccagccct
     2821 agccggaaag gcaagggtga gcttagctaa ccgttcggcg atcccgctca tctcctggtt
     2881 gagggctgcg cggctggtct ctgcgcgggc gcggatgtgc tccacctgtt tcaaagcccc
     2941 gggggtagcc agcactgcca gaccttgcgg gatcaggtag ttgcgaccat agccatcggc
     3001 gactcttttg acatcgccag cgcgaccaag tttatacacg tcttccaata acaaaacttt
     3061 catttctttc tcaagccctt tcaatagaat gtctcgacat acaaacccgg gcgaagggta
     3121 caacgctccg ggcaggggga ttttaccaga tattttaaga aattgggcac aaaccgctct
     3181 ccatcccgca aacgcccgat ttcgccatta taatcaagcc atgccttcca gagaccgctc
     3241 cttccgtgtc gaggcggtgg tgctgcgcca cagtgactgg ggtgaggctg accgcctgct
     3301 gtggctgttc acgcgcgaga tgggcaaggt gaaagcggtg gcaaagggcg tccgcaagct
     3361 gcgttctcgc aaggcaggtc acctcgagcc gcaaacgcgc gtcaacctgc tgctggcacg
     3421 cgggcgcgat atcctgcttg tcacccaggc agagacagtg gaagccttcc tgcccttgcg
     3481 ggaagatctt aagctgtggg agtatgcctg ccacattatc gagctgcttg accgtttcac
     3541 gtatgaagaa gacgagaatc gggctctata ccgcttgctt gtggacacgc tagggcggtt
     3601 agccagacag ccgcaagcgg aattggtggt gcactactac gagatacgcc tcttggattt
     3661 actgggctac cgcccgcatc tatttcactg cgcaaactgc agcaaagaga tccagccaga
     3721 ggaccagtat ttctccgcag atcagggggg tgtgttgtgc ccatcttgcg ggcgagaggt
     3781 gaaaggtgcg cgtcccatca ccatgtcggc gttgaaatac ctgcgccact tccagcgcag
     3841 cagttttagc gaggccgctc gtgcccgcat cccccacaac gtgatgcgcg agatcgaggc
     3901 gttgatggaa tattatttga cttatttgtt agagcagggg ttaaattcac tggcgttcat
     3961 tcgcaggatg agaaggggag agatgagcaa caaagcgaga gatcaagaaa ttatatgaac
     4021 tggtcatccc cttcgctcaa ctcactagct aactgtccaa taccgttgta aattatcatc
     4081 aagatgggta caatggacga tgatatggag gaattatgtc ggattttgat ctggcacccc
     4141 tgcagcctgt attagctgaa ttcactaccc aggggcgcac tgccctatta cccgccctgc
     4201 atgccgccca gaaattgtac gggcacctgc ccgagccggt cgccgccgag gtaggccgcg
     4261 ccctgggtgt gcccttagcc gatgtgtacg gcgtgatcga cttttacagc atgttctaca
     4321 gccatcctac cggcaagacg atggt
//
LOCUS       DBNGSX010000114         3239 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1739731, whole genome shotgun sequence.
ACCESSION   DBNGSX010000114 DBNGSX010000000
VERSION     DBNGSX010000114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1739731"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1948
                     /locus_tag="AC16CB_03845"
     CDS             <1..1948
                     /locus_tag="AC16CB_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006727758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HIR3341635.1"
                     /translation="VPQGINLLKAAQELGVELESICGGRQTCGKCQVVVEDGHFPKHA
                     LTSRADHLSAPTTREIEYSQEHRLQGRRLACAAEVLGDVLISVPEESQARKQVIAKAA
                     SERSIEIDPAVRQVYVEMQPSDLSDSRGDWERLQEALEAQWGLKGLDIDSSALATLQI
                     ALREGDFSVTVNVWQEREVLRIQPGYQEGTYGLAVDIGSTTVVAHLCDLRTGAVLATE
                     AAMNPQVRYGEDLMSRVSYANSEPQGLSRLHRAVIRTLNELAENATKAAGLSPEDILD
                     AVIVGNPVMHHLFLGIHPRELGAAPFAPAVSSALDLKARELGLAFHPAARLHTLPLIA
                     GHVGADNVAVLLAEAPNEQDDVMLIVDVGTNAEIVLGNRQRLLAASSPTGPAFEGAQI
                     THGQRAAPGAIERVRIDPKTLEPRFKVIGYESWIEPGAELPHEAKPTGICGSGIIEAV
                     SEMLLAGIIRPDGLFDRSMTDRSPRVNYQGRTGEYILADASLTATGLAIVITQNDIRA
                     IQLAKAALYAGTKLLMNHWGAERVDKVVLAGAFGSFISPYHAMLLGLIPDCDLENVYA
                     VGNAAGDGARFALLNRNLRSKAAQLALWVEHVSTPLESSFQDEFVAALALPHARDPFP
                     TLQAFLPQVEGDPKPGRRNRVRDR"
     gene            2097..2789
                     /locus_tag="AC16CB_03850"
     CDS             2097..2789
                     /locus_tag="AC16CB_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009759767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HIR3341636.1"
                     /translation="MSDEIDISQLETPEILELIQDDLYDGLADEVVKSVKELLSRSMT
                     PYDVLTKGLVAGMDVVGVDFRDGILFVPEVLMAAKAMKAGMEILRPLLAETGAPQVGT
                     MVIGTVKGDIHDIGKNLVAMMMEGGGFTVVNLGINVDADTFIKAIHEHKPEILGMSAL
                     LTTTMPYMGVVIETLKEQGLRDQLIVLVGGAPLNDAFAEEIGADAYCRDAAVAVETAK
                     KLLAIKRGEPIG"
     gene            2866..>3239
                     /locus_tag="AC16CB_03855"
     CDS             2866..>3239
                     /locus_tag="AC16CB_03855"
                     /inference="COORDINATES: protein motif:HMM:NF014615.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR3341637.1"
                     /translation="MTTIPSLLEKSPYILADGAMGTMLFASGLEQGGAPELWNVEQPE
                     RVGAVHRAYLEAGAQLLLTNTFGGNRHRLTLHKLQGRVAELNRAAVKILRDVVDASGV
                     EALVAGDIGPSGGVLLPYGTMT"
BASE COUNT          676 a          868 c         1028 g          667 t
ORIGIN      
        1 cgtgcctcag ggcatcaatt tgctcaaagc agcccaggag ctcggcgtgg agctggaatc
       61 aatctgtggt gggcggcaaa cctgtggcaa gtgccaggtt gtcgtcgaag atgggcattt
      121 tccaaagcat gctctcacct cgcgcgccga tcacctttct gctcctacca cccgcgagat
      181 cgaatatagc caggagcacc gcctgcaagg gcgtcgcctg gcatgcgctg ccgaagtgct
      241 gggtgacgtg ctgatctcgg ttcccgagga gagccaggcg cgcaagcagg tgattgccaa
      301 ggctgcttct gagcgcagca tcgagattga tcctgctgtg cgtcaagtgt acgtagaaat
      361 gcagccatca gacctgagcg attcgcgcgg tgactgggag cggttgcagg aagcattgga
      421 agcacagtgg gggttgaagg ggctggacat agatagtagc gccctggcga ctcttcaaat
      481 cgccttgcgt gagggcgatt tttcagtgac agtgaacgta tggcaggagc gcgaagtgct
      541 gcgcatccag cctggctatc aagaagggac gtacggcctg gcagtggata ttggctcgac
      601 caccgtggtg gcccatttgt gtgacctgcg caccggcgct gtgctggcaa cagaagcggc
      661 catgaacccc caggtacgat acggcgaaga tctgatgagc cgggtgagtt acgccaatag
      721 cgagccccaa ggtctctcgc ggttgcaccg ggcggtgatc cgcacgctca acgagctggc
      781 ggaaaatgcg accaaagccg ccgggctctc cccggaagac atcctggatg cggttatcgt
      841 gggtaatccg gtcatgcacc acctgttcct gggcatccac ccccgtgagt tgggagcggc
      901 gccgtttgcc cctgcggtga gctctgccct agacctaaaa gcgcgtgagc tgggcctggc
      961 attccatccg gcagctcgct tgcacaccct gccgctgatc gctgggcatg ttggggcaga
     1021 taatgtggcg gtgttgttag ccgaggcgcc aaacgaacag gatgatgtca tgctgatcgt
     1081 ggatgtgggc acgaatgccg agatcgtatt aggcaaccgc cagcgccttc tggcagcctc
     1141 aagccccacc ggaccagcct tcgaaggcgc gcagatcacc catggacagc gcgctgcgcc
     1201 gggagctatt gaacgggtgc gcatcgaccc aaagacactg gaaccacgct tcaaggttat
     1261 cggttacgaa tcctggattg aaccgggcgc agagctaccc cacgaggcca agcccacggg
     1321 tatctgcggc agcgggatca ttgaggcggt ttcggagatg cttctggctg ggattatcag
     1381 gccggatggt ctcttcgatc gctccatgac ggatcgctcg ccgcgcgtga actaccaggg
     1441 caggacgggc gagtatatcc tggccgatgc cagcctgacg gctacaggtc tagcgattgt
     1501 catcacgcag aatgacatcc gcgccatcca gctggccaaa gcagccctgt atgccggaac
     1561 gaagttgttg atgaaccatt ggggcgcgga gcgggtggat aaggtcgtgc tggcgggcgc
     1621 ttttggtagt tttatcagcc cgtaccatgc catgctgctg ggcttgatcc ccgattgcga
     1681 cctggaaaat gtttacgcgg tgggtaacgc cgccggcgat ggggcgcgct tcgcgctgct
     1741 caatcgcaac ttgcgctcga aagcggccca gctggctctg tgggtggagc acgtttcgac
     1801 cccattggaa tcgtcttttc aagatgagtt tgtggctgct ctggcgttgc cacatgcccg
     1861 cgatcctttc cccactttgc aagcattttt accgcaagtg gaaggagatc caaagcccgg
     1921 tcggcgcaat cgtgtgcggg accgataata gttaaatttg ttgaagctac cctgtggtca
     1981 acactggaat gggtagcgaa aaaaagcaag aatgcctcgt gggcgccttt gtgcccgcaa
     2041 cacacaaacg ttgatttgtg tataatacct aaactgctcg aaggagtact taatacatga
     2101 gcgacgaaat cgatatttct caactggaga ccccagagat acttgagttg atccaggatg
     2161 acctgtacga cggcctggca gacgaggtgg tcaagagcgt caaggagctg ctctcgcgca
     2221 gcatgacccc ttacgacgtg cttaccaagg gattggtagc cggcatggat gtcgtgggcg
     2281 tggactttcg cgatggcatc ctgtttgtgc cagaagtgct gatggctgcc aaggcgatga
     2341 aggccggcat ggagattctg cgcccgctgc tggccgagac cggcgcgcct caggttggca
     2401 ccatggtgat cggcaccgtc aagggcgata tccacgatat tgggaagaac ctggtggcaa
     2461 tgatgatgga aggcggcggt ttcaccgtgg tcaacctggg catcaatgtg gatgccgata
     2521 cgtttatcaa agccatacac gaacacaaac cggagatcct ggggatgagt gctctgttga
     2581 ccaccaccat gccctatatg ggcgtggtta ttgaaaccct caaggagcag gggctgcgcg
     2641 accagttgat tgtattggtg ggcggggcgc cgctcaacga tgccttcgcc gaggagatcg
     2701 gagcggatgc ctattgccgc gatgcagccg ttgcagttga gacggctaag aaactgctgg
     2761 cgatcaaacg cggcgagccg atcgggtaat aatacggcgg cattggaaag acgctaattg
     2821 actatgccgg gttgcatctc ggcagctttg gaaaggcagg ataccatgac aaccatccca
     2881 tctcttctcg aaaaatctcc ctatattctg gcggatgggg ccatgggcac catgctcttt
     2941 gcgtccggtt tggaacaagg tggtgcgcca gagctgtgga acgtggagca acctgaacgg
     3001 gtgggagcgg tacaccgcgc ctaccttgag gctggcgcgc aattgttgct taccaacacc
     3061 ttcggtggca atcggcaccg cttaacgttg cacaagctgc aggggcgcgt agctgagctt
     3121 aatcgcgccg cggtgaaaat tttgcgtgat gtggtggacg ccagcggggt ggaagccctt
     3181 gtggctggcg atatcggccc ttcgggcggg gtgttgctcc cctatgggac gatgaccta
//
LOCUS       DBNGSX010000115         3601 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_8633, whole genome shotgun sequence.
ACCESSION   DBNGSX010000115 DBNGSX010000000
VERSION     DBNGSX010000115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_8633"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="AC16CB_03860"
     CDS             <1..208
                     /locus_tag="AC16CB_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF268 domain-containing protein"
                     /protein_id="HIR3341638.1"
                     /translation="FNAHRIFSVSTILELARERMELIDFSYVDDRGDLHTQAALTTSN
                     IETSFGCQYGCGIFEFRKRAGTIH"
     gene            205..1128
                     /locus_tag="AC16CB_03865"
     CDS             205..1128
                     /locus_tag="AC16CB_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341639.1"
                     /translation="MIGIIFSRDRAFQLDGTLRSLLWHCQDTNVLELFILYKATSEKH
                     MRQYEQLKKQYTAKNIRFLAERSFKEDILKLLFTPSLSLWEQSRTYLSGRLGEWFGHP
                     FSVLMPFGRENTILFLVDDNIFVRAFRLAEVTAALDANSTVLGFSLRLGPNTSYCYPL
                     DQQQTPPDFRPVNDHVLEYHWGGAEGDFGYPLEVSSSIYRIKELLPLLVIMPYTNPNQ
                     LEAQLARRAKKFRRKAPLLACFRQSVTFCNPVNRVQTVLNNRVGSNQEFSNENLANMF
                     DADYRLDVDAYKDFVPVSCHQEVDLTFRKVL"
     gene            1125..2300
                     /locus_tag="AC16CB_03870"
     CDS             1125..2300
                     /locus_tag="AC16CB_03870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3341640.1"
                     /translation="MTAAVHQEKPSILFLGSQMATGGAQHLLLEQARWFHQRGHRVVA
                     AYFYDKEGHYEVWREQAPYPLINLNGWRKEKSLVNGLRLTRALIQLYFFLKKEKFTVV
                     ETFTHSANILGLPLAWAAKIPVRIASHHGRFEGLTSLQERFHRVIINSGLATRMVAVS
                     SCVAELATEKEHINPNKITVIRNGIDMSVEVNLDEEERISLRQSLGVGTNDFLIITVG
                     RLVNQKGHVYLLEAMAEVIKHFPHTKLVIAGNGPLQATLVAKARTLQIEKSVHFAGNR
                     GDVPYLLSIGDCFVLSSLWEGLPIALLEAMRAGLAIIATKVEGITEVITDGINGLLVP
                     AADARALAQSIMRIISDDVARERIGRQGRTEVMRTYSYERMCEEYATLFQRELEARG"
     gene            2297..>3601
                     /locus_tag="AC16CB_03875"
     CDS             2297..>3601
                     /locus_tag="AC16CB_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341641.1"
                     /translation="MKEKARAINFLFRLAATYFICKVLIEAANEYFEYAWGYGSWLGG
                     FTLNGAIGLIALVLAWAMLFALIMLRLWLYDRTIRIEGVLSSWRNRLGPLCGLIIIIP
                     LLTPLWFMQYSRWGGEFIGLPFRLVVFLVSSYMVCVLLTRSHTKLVTLPNLAISILLV
                     TSGLIIGSTAAGITDYPFSLTWSEGNRMWDYSVLFGRAIYDYPSDRPIFAYLDLGRQS
                     LWGLPFLLPNVSIVAVRAWNAFLLTIPYAIFGWIVFMKGAKLGKLWMLAGLWSLAFMS
                     QGPIYTPLVLGAIFVAIAWFTPHWMAFILIAVAGYYLNIGRYTWIFAPAMWASMIYFG
                     EIKISVSKFDPKRVMPAIGAVLAGLFGGYVLPRWQVIFQAATSILRPSDISGQAAAPG
                     MDIVSVEGVSSMISRQPLLWGRLLPNPTYELGILLGLLVATLP"
BASE COUNT          901 a          812 c          879 g         1009 t
ORIGIN      
        1 attcaatgcc caccgaatat tttctgttag cacaatcctg gagctagcca gagaaagaat
       61 ggaattaatc gacttttctt atgtagacga tcgaggagat ttgcacacgc aagctgcact
      121 cacaacaagc aacatcgaga cctctttcgg atgtcagtat ggatgtggta tttttgaatt
      181 ccgtaagcgg gcaggaacaa ttcattgatt ggtatcattt ttagccgcga tcgcgcattt
      241 cagttagatg gcacactgcg ctctttgctc tggcactgcc aggacacaaa tgtgctggag
      301 ctatttatct tatacaaggc tacgagcgaa aagcacatgc gtcaatatga gcaactaaaa
      361 aagcaatata cagcaaaaaa tattcgtttt ttagcagaac gctcttttaa agaggatatc
      421 ctaaaactct tatttacacc ttcgttgagc ttatgggaac agtcacgtac ctacttatct
      481 gggcgactag gtgagtggtt cggtcatcct ttttctgttc ttatgccatt tggccgggaa
      541 aacacaatcc ttttcctggt tgacgacaat atcttcgtgc gtgcatttag gctggccgaa
      601 gttactgctg cgctcgatgc caactcaact gtcctgggct tctcattacg tttgggacca
      661 aatacgtcct attgttatcc cttggatcag caacagacac ccccagattt tcgcccggta
      721 aacgatcacg tcctggaata ccattggggc ggtgcagaag gtgattttgg gtaccctcta
      781 gaagtatcca gctccatata ccgaatcaaa gagctgctcc cactactggt aattatgcct
      841 tatactaacc ccaatcagct ggaagcgcaa ttggcccggc gtgcaaagaa gtttcgccgt
      901 aaagcccccc tgttagcgtg ttttcggcag agtgtgacgt tctgtaatcc agttaatcga
      961 gtccaaaccg tgctaaacaa ccgggtaggt agcaatcagg aattctccaa tgagaatctt
     1021 gccaatatgt ttgatgctga ttatcgcctg gatgtggatg catataagga ctttgtgccg
     1081 gtctcttgcc accaagaagt ggatttgaca ttcaggaagg tattatgacg gcagccgtgc
     1141 accaggaaaa accatccatt ctctttctgg gctcccagat ggcgaccggc ggagcccaac
     1201 accttctatt agaacaagct cgctggtttc accaacgagg acatcgggta gtggccgctt
     1261 atttttatga taaagagggc cattacgaag tttggcgtga gcaagcacca tacccgttga
     1321 tcaatctcaa cggctggcga aaggaaaaga gcctagtcaa tggattacga cttacccgtg
     1381 ctctaataca gctctatttc ttcctaaaaa aagaaaagtt tactgtggtt gagactttta
     1441 cccatagcgc caatatttta gggttacctt tagcatgggc ggcaaaaata cccgtgcgca
     1501 tcgcctcgca tcacggtcgt tttgaagggc ttacctcgct gcaagagcgc ttccatcggg
     1561 tgattattaa tagcggctta gccacgcgaa tggtggcagt ctcaagctgt gtagctgaac
     1621 tggctacaga aaaagaacat attaacccca acaaaatcac agtgatacgt aatggcatcg
     1681 atatgtcggt ggaagtgaat ctagacgagg aagagcgaat ttctctccga caatcgctag
     1741 gagtcggcac caacgacttt cttatcatta ctgttggtcg cctggtaaat caaaaagggc
     1801 atgtatattt acttgaggca atggcagagg taataaagca ttttccccat accaaactgg
     1861 taattgcagg taatggcccc ctacaagcaa cattggtggc gaaagctcgt acattgcaaa
     1921 tcgaaaaatc ggtccatttt gccggtaacc ggggtgacgt gccatatctg ctaagcattg
     1981 gggattgttt tgtattatct tctctctggg agggtttacc gatcgcccta ctcgaagcca
     2041 tgcgtgcggg tcttgcaatc atcgctacca aagtggaagg catcacagag gtgatcacag
     2101 atggcataaa tggtttgctt gtgcctgcgg cagatgcaag ggctctcgca caatccatca
     2161 tgcggataat atctgacgat gttgcccggg aaagaatcgg cagacagggt agaaccgagg
     2221 tcatgagaac atatagctat gaacgaatgt gtgaagaata tgcaacttta ttccagcgag
     2281 agttggaggc gcgaggttga aagaaaaagc ccgagccata aattttctat ttcgccttgc
     2341 agcaacttat ttcatttgta aagttctcat cgaggcagca aacgagtatt ttgaatatgc
     2401 ttgggggtat ggtagttggc tgggtggctt cacgctaaat ggggctattg gattaattgc
     2461 gcttgtgctg gcatgggcta tgctgtttgc tctcatcatg cttagactat ggctgtacga
     2521 tcgaacgata cgcattgaag gagtattatc gtcctggcga aatcgcctag gtccgctgtg
     2581 tgggttaata atcattattc ccttactaac cccattatgg tttatgcaat attcccgatg
     2641 gggtggtgaa tttattggat tgccattccg cctggtggta tttttagtgt cgagttatat
     2701 ggtttgcgta ttattaacac gaagccacac caagttggtc accttgccca atctagccat
     2761 tagcattctc ctggtgacca gcggcttaat aattggaagc actgctgcgg ggataactga
     2821 ttatcctttc tctttgactt ggtcagaagg caaccggatg tgggattatt ccgtattatt
     2881 cggacgcgca atttacgatt accccagcga tcgacccatt tttgcctatt tagatcttgg
     2941 aagacaatcg ctttggggac tcccatttct cctaccgaat gtctctatcg tggcggttag
     3001 agcttggaat gctttcctcc tcacgatacc ctatgcaatt ttcggctgga tcgtttttat
     3061 gaaaggcgca aagttaggga agctttggat gttggctgga ttatggagcc tggcttttat
     3121 gagtcagggt ccaatctaca cccccttggt actaggtgct atcttcgttg cgatagcttg
     3181 gtttactccg cattggatgg cctttatctt aatcgctgtg gctgggtact acctcaatat
     3241 cggtcggtac acctggatat ttgcgccagc aatgtgggcc agcatgattt attttgggga
     3301 gataaagatc tccgtttcga agtttgaccc taagcgggtc atgccagcca ttggagctgt
     3361 tctggcggga ctttttggag ggtatgtcct gcctcgctgg caagtgattt tccaggcagc
     3421 gacttcaatc ttaaggccta gcgatatatc tggccaggct gccgcgccgg ggatggatat
     3481 tgtcagcgta gagggagtgt cttcaatgat ctcgcgccaa ccgctcctat gggggcgttt
     3541 gctaccaaat ccaacctatg aactagggat tttactcggt ctcttggttg ccactttgcc
     3601 a
//
LOCUS       DBNGSX010000116         6733 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1393637, whole genome shotgun sequence.
ACCESSION   DBNGSX010000116 DBNGSX010000000
VERSION     DBNGSX010000116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6733)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1393637"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2337
                     /locus_tag="AC16CB_03880"
     CDS             <1..2337
                     /locus_tag="AC16CB_03880"
                     /inference="COORDINATES: protein motif:HMM:NF037660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3341642.1"
                     /translation="WKISKSYWMQRNSRLFKYILDEVTNALARLEEPAILVLENYDLI
                     TSPLIHEMPKYFLKHQPHQAHLVVITRSDPPLPLARLRARRQMVEIRASDLRFTEEEA
                     GHFFSQSMQLVLEEEDIHSLEMRTEGWAVGLQLAALALKNLPDPQNFVETFRGSHRYV
                     LDYLAEEVIRQQRDDIREFLMQTSILERLNAESCEALTGYPNSQGLLSELEQANLFLI
                     PLDDERVWYRYHHLFADFLRTELSKTETEKLYKKAALWHEQNGLLSEAVQYAIASGDL
                     EFLADVIDRGLKKDAIWSGGNLTLYATWLDALPPQAFQSRPALSLNASHILYLLGRFD
                     LAEKQIDQAEQTLHALPSSPEKEQMLALASLYRGSIASVRGETQQAIDKITFAQERLP
                     QEYHLQHARGFFSLGLAYELSGETALAGQNYLKSSEEAQSAGVLYLAIHALGAAAQVQ
                     ISQGQLHLAEQACQGVIQIAGGKRLPPLGLVETILGSIALERNDLAAAEENLQKGIAL
                     SRRGGLIDDVILGLSYLARLHAYQGRVSNAFETAQELNTIIQGFGVERMSMLAAAYIA
                     NLQLYTGQERAATQWAMEYQADRPDQPHEVAELTLVRVLLKAGDRENVPSILDALLKR
                     GQAQGRVRTCMEVMILMALFHRAEKDIPAAIDWLSQALGLAAPEGFVRIFLDEGEALL
                     DLLPKARQAAPELVDAILGNLQAESGKSPLDQLPDPLSEQEIRVLGLIVAGNSNKEIA
                     GELVISVGTAKWHVHNILQKLGVSNRSQAIARARELGF"
     gene            2475..3128
                     /locus_tag="AC16CB_03885"
     CDS             2475..3128
                     /locus_tag="AC16CB_03885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3341643.1"
                     /translation="MKINQLLNLGTISGIVILIVAVFLVWRFISNRRSLPCPSWLGWM
                     VEMDNPFTKTNRASVIIDLLGLQPGMKVLDAGCGPGRLAIPAAKAIGTQGELTALDLQ
                     AGMLARVQEKTRTAGLSNIRLMQAGLGEGKLEKAYYDRAMLVTVLGEIPEQEAAMQEI
                     YQALKPGGILSVTEVIFDPHFQSSKSVKQVAGKVGFREVGFFGKRLAYTMHFEKKNQ"
     gene            3162..5480
                     /locus_tag="AC16CB_03890"
                     /pseudo
     CDS             3162..5480
                     /locus_tag="AC16CB_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009677144.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            5484..6248
                     /locus_tag="AC16CB_03895"
     CDS             5484..6248
                     /locus_tag="AC16CB_03895"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015736594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3341644.1"
                     /translation="MNKILVGENIVKSFGEGAEKRNVLDGVSVEVNEGEFVSVMGPSG
                     SGKSTLMYALSGMDGVDSGKVAFDCRELSTLSENELADIRRRNMGFVFQQPTLLKNLT
                     ILDNIILPSMRDQRDEAAKLTKKARTLMKQADILELENRTITQASGGQLQRVGICRAL
                     MNNPKIIFGDEPTGALNSTSANEIMQMLAEIHQSGTTIMLVTHDVKVAAKTERVLFLF
                     DGKIAGEYFVGAYDQAGDDLKAREERLTAWLADLEF"
     gene            6405..>6733
                     /locus_tag="AC16CB_03900"
     CDS             6405..>6733
                     /locus_tag="AC16CB_03900"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3341645.1"
                     /translation="MATQFSVQDNKPWKTTFFTIWSGQALSILGSQLVQFALIWHLTV
                     QTGSATVLVTASLAGMLPNVVLGPFIGTLVDRWDRRRIMLIADSIVALATIVLAVLFA
                     LDVVEVW"
BASE COUNT         1659 a         1680 c         1779 g         1615 t
ORIGIN      
        1 tggaagatat ccaagagcta ttggatgcag cgcaactccc gccttttcaa gtatattctg
       61 gatgaagtca ccaacgctct tgcacgtctt gaagagccag ccatccttgt cctggagaat
      121 tacgatctca tcaccagtcc gctcattcac gagatgccga aatattttct caagcaccaa
      181 ccccatcagg cacatctggt cgtcatcacg cgctcagatc ccccgctccc cttggcacgt
      241 ctaagagccc gcaggcaaat ggttgagatc cgcgccagtg acctgcgctt cacagaggaa
      301 gaagccggtc atttcttcag tcaatccatg caactcgtgc tggaagagga agacatccac
      361 tctttggaaa tgcgcacgga gggatgggca gttggcttgc aactcgcagc attggcgctc
      421 aaaaacctgc cagatccgca aaattttgta gaaacattcc gcggcagcca ccgctatgtg
      481 ttggattact tggcggagga agtcattcgc cagcaaaggg acgatattcg cgaatttctt
      541 atgcagactt ccatcttgga acgcttaaat gccgaatcct gcgaagcact cactggatac
      601 cctaacagcc aaggcttgct ttcagagctt gaacaggcga acctgttcct gatcccactg
      661 gatgatgaac gcgtctggta ccgttaccat cacctttttg ccgatttcct gcgaacggaa
      721 ctctccaaaa ctgaaaccga aaaactgtac aagaaggctg ctctttggca cgaacagaat
      781 ggcttgctct ctgaagcggt gcaatatgcc atcgcaagtg gcgatcttga atttttggcg
      841 gatgtcatcg accggggatt aaaaaaagat gccatttggt cgggaggaaa tctgacgttg
      901 tatgcaacct ggctggatgc gctcccccca caagcctttc aaagccgccc cgcattgagt
      961 ttgaatgctt cgcacattct ctacctgtta ggacgctttg atcttgctga aaagcaaatt
     1021 gatcaagccg agcaaacctt gcatgccctg ccctcctcac ctgaaaaaga gcaaatgctg
     1081 gcactggcat cactgtaccg cggctcaatc gcctccgtgc gtggggaaac gcaacaagcc
     1141 attgataaga tcactttcgc ccaggaacgg ctcccacaag aatatcattt gcagcacgca
     1201 cgcggattct ttagtctggg gctggcatat gaactctccg gagagaccgc acttgccgga
     1261 cagaactatt tgaaatcaag cgaggaagcg caatccgctg gggtgcttta tcttgccatc
     1321 catgcgcttg gcgcagcggc acaggtgcag atcagccagg gacagttaca cttggcggag
     1381 caagcctgcc aaggtgtaat tcaaatcgca gggggcaaac gattaccccc actgggtttg
     1441 gtagagacta ttctaggcag cattgctctc gaacgaaacg atctcgccgc cgccgaggaa
     1501 aacctgcaaa aaggcattgc gttatcccgt cgagggggcc tgatagatga cgtcatatta
     1561 gggctatcat atctcgcccg tttacatgcc tatcaagggc gcgtatcgaa tgccttcgag
     1621 actgcccagg aattgaacac gatcattcaa ggcttcgggg tggagcgtat gtccatgctc
     1681 gcggcagcct acatcgcaaa cctgcaactc tacactggtc aagaaagggc agcaacccaa
     1741 tgggcgatgg aataccaagc tgaccgcccg gaccaaccgc acgaagtcgc ggagctgacc
     1801 cttgtgcgcg tcctgctgaa agcgggcgat cgcgaaaatg tgccgtctat cttggatgcc
     1861 ctgcttaagc gagggcaggc tcaaggtcgc gttcgaacct gcatggaggt catgattctg
     1921 atggcattat tccatcgtgc cgaaaaagac atcccggccg ccatagactg gctatcccaa
     1981 gccctgggat tggcggcacc cgaaggcttt gtccgcatct ttttggatga aggggaagcg
     2041 cttctcgatt tgttgcccaa agcgcgtcaa gccgcccccg aacttgtaga cgctatcctg
     2101 ggtaatcttc aggctgaatc gggaaaatcg cccctagacc agttgcctga tcctctcagt
     2161 gagcaagaaa tacgggtatt gggcttgatc gttgcaggaa attccaataa ggaaatcgca
     2221 ggcgagctgg tcatcagtgt ggggactgcc aagtggcacg ttcataacat cctgcaaaag
     2281 ttgggtgtta gcaatcgttc gcaggccatc gcccgggcca gggaattggg cttctaaatg
     2341 tcctcttctg attcctgaga gccgttcttc gcggctcttt tttatttaac ctacccccac
     2401 aacctatctt ccgatagggg acaaaaactg tgccagattg taaactaggg ctgtaaaaac
     2461 agaaggagta aatcatgaaa atcaatcaat tactcaacct cggcacaatt tctggcattg
     2521 tcatactgat tgtagccgtt tttctagttt ggcggttcat atccaatcgc aggtcgctgc
     2581 cttgcccgtc ttggctgggt tggatggttg agatggacaa cccatttact aaaacaaacc
     2641 gcgccagtgt cattatcgac ttgttgggtt tacagcctgg tatgaaggtg ctggatgccg
     2701 gatgcggacc ggggcggcta gcaattcccg ccgcgaaagc cattggcacg cagggcgagc
     2761 tgaccgcgct tgatctccaa gctggaatgt tggcccgcgt gcaggaaaaa actcgcactg
     2821 ccggactgtc caacatccgc ttgatgcagg ccggtttggg cgaaggcaaa ttagaaaagg
     2881 cttactatga tcgtgccatg ctggtcacgg tgctggggga aattcccgaa caggaagcag
     2941 ccatgcagga aatttaccag gctctcaaac cgggcggcat tctctcggtc acagaggtca
     3001 tctttgatcc gcacttccag agcagcaagt ctgtgaagca ggtggcaggc aaagtcggat
     3061 tccgtgaagt gggtttcttt ggaaaacggc tggcttacac catgcacttt gagaaaaaga
     3121 accaatagcc gtgtgttgat acagatattc agaggtgaaa gatgtattac agaataatcc
     3181 gcaacgacat tttcagaaat cgagcgatca cactgacaat catcatgttt gtcgcggctg
     3241 cagctatgct ggtttcgctg gcggcgatac ttgtcgtaaa cctttcggga gcgatcgaca
     3301 cgctgatgaa ccaggcgaaa acgccgcatt tcatgcagat gcattcgggc gaaatggact
     3361 ctgcccggct ggcagctttt gttgagcagc acagcgatat taatgaatat caagtgcttg
     3421 aattcctaaa cgtcgatggc gcgcagatca tttttgacga aggttcgctg gcgggtagtg
     3481 ttcaggataa tggcttctct gtgcagagcg aaaaattcga ttatcttctc gatctcgacg
     3541 gcaatgtcat ccaggtatcc gatggtgaga tttatgttcc gataccctat atgcagcaag
     3601 gcctcgcaaa tatcggcgac acggtttagg tatccggaaa gggatttacc gttgcgggat
     3661 ttgtccgtga ttcacagatg aattcgctgc tttcatcatc aaaaagattc ctggtgagca
     3721 aaaatgacca cgctgaacta agggaattcg gaagcgtaga atacctgatc gagttcaggt
     3781 taaccgactt atccgagctg ggcgcatttg aaaccgcgta cacctccgcg ggcttcgaag
     3841 caaacggacc aacgatcaca taccctcttt tcaagttgtt gaacggtctt tccgacggac
     3901 tgatgatcgg ggtcattcta ctggtcagtg ccctggtgat cgccgtcgcg ttcctgtgca
     3961 tccgcttcac gctgctggcg aaaatcgagg aggattaccg ggaaattggt gtgatgaaag
     4021 cgatcgggct gcgcgtatcc gatatcaaga taatatacct tgttcaatac gcagcgattg
     4081 cggcattggg cagtatcctc ggatttgcgc tctcatttgt atttcggggc atgcttcttg
     4141 agaacatccg cctctatatg ggtgaaagcg aaagcgcttc ctttgccatg ctcttcggga
     4201 tgatcagcgt gctgcttgtg ttcctggcga ttatcgctta tgtgaacggt gtgctcaggc
     4261 gcttccgaaa aattcccccg gcggaggcga tccgcttcgg gatctcacag gaaaagtccg
     4321 gcgggggaaa gtttttccga ctgagcatga acagactgct gggtacaaat gtcttcctgg
     4381 gattgaaaga tgttctggcg agaagaaagc tctatgccac gatgctcgcg gtgctggtga
     4441 tttcggcctt catcatcatc gtcccgcaga atttacacaa caccatttct tcaaacgact
     4501 tcatctctta tatggggatc ggaatgagtg atctgcgcat tgacattcag cagaccgatc
     4561 atatttctga aaaagcggca gaaatcgcag ccgcaatgga aaccgacagc gcaatatcca
     4621 gatatgtcgt gctcaccacc aaaacattca gtgtcaagat ggatgacggc tcggaggaac
     4681 ggatcaagat tgaactgggc gaccattcgg tcttccccct gacatatttc gcaggtcgcg
     4741 cacctgctga agagaatgaa attgctcttt cggtgatgaa cgccgatgag atgggcaaga
     4801 cggtggatga tgtcatcctg ataatggtcg aaggacagga aaaagctttc actgtgagcg
     4861 gaatttactc agatattact aacggtggta agaccgcaaa agcggttttc tccaatacgt
     4921 cggcagacac gatgtggatc gtgatcggtg ctgaactttc ggatggatcc ctgatcgacg
     4981 caaaaattgc cgaatatgca gacaggtttg aatttgcaaa ggtttctgga attgatgaat
     5041 atatcgcaca gactttcggc gcgaccatcc gttccgtcgg aacagcttcc tatgccgcaa
     5101 ttgtcgttgc gatgacgatc acggtgctga tcacgctgct atttatgaga atgctggtta
     5161 caaaggatcg atactcgatt gcggtgatga aggcctttgg ttttacgaac tcggacattc
     5221 agacacagta tattgcgcgt tcggtattcg tcctgatcgt tggcgttttg ctcggcgcgc
     5281 tcctggcaaa tacgctcgga gaagcccttg cgagcgcggt gatcgcctca gttggagcat
     5341 catcattcaa gtttgtggtc aatccgcttt cagcgtatct gctgagtccg ctgttgatga
     5401 tgggtgccgt gcttgctgca acatacctgg gcacactgga tgcaggccaa atccaaattt
     5461 ctgaaaacat aaaggagtaa atcatgaata agatcctggt cggtgaaaat atcgtcaagt
     5521 cttttggcga aggcgctgaa aaacgaaatg tgctggatgg ggtctcggtc gaggtcaacg
     5581 aaggcgagtt cgtctcggtg atgggacctt caggttcggg aaaatcgacg ttgatgtatg
     5641 ccctgagcgg aatggatggt gttgacagcg ggaaggtcgc gtttgactgc agggagttat
     5701 cgacactcag tgaaaatgag cttgccgaca tccgcagaag aaacatgggg tttgtttttc
     5761 aacagccgac tttgctgaaa aacctgacca tcctcgacaa catcattctc ccgtccatgc
     5821 gagaccagcg tgatgaagcc gcgaaactca ctaaaaaagc gcgaacgctg atgaaacagg
     5881 ccgatattct ggaactggaa aaccgcacga ttacccaggc ttcaggtggg cagctgcagc
     5941 gtgtaggaat ctgccgcgca ctgatgaata atccgaaaat tattttcggt gacgagccaa
     6001 ccggcgcttt gaattccaca tctgccaatg aaatcatgca aatgctggcc gagattcatc
     6061 agagcggcac cacgatcatg ctggtcacgc atgacgtgaa agtagcagcc aaaacagagc
     6121 gggttctttt cctgtttgat ggcaagatcg cgggagagta tttcgtgggt gcatatgatc
     6181 aggccggcga cgatcttaaa gcccgcgaag aacgtctcac cgcctggttg gccgatttgg
     6241 aattctgatt tcctgtcgtc agcctggctt ttgtcaatcg ctgaattcat ctgtgaaacg
     6301 gatggaactg cgaaaaaacg gtttcccgga actatccttg agggcgaaga gatgcgctct
     6361 tgcccctaaa gatgatccca aatggaaaag caaggagttc tctcatggca acacaatttt
     6421 cagtccaaga caacaaaccc tggaagacca ccttctttac catctggagt ggacaggcgc
     6481 tttccatttt aggcagccag ctggtgcaat tcgccctgat ctggcatctg accgtccaga
     6541 cgggttcagc cactgtgctg gtgactgcct ccctggccgg gatgctgccc aacgtagtcc
     6601 tcggcccatt catcggaacg ctggtcgacc ggtgggatcg tcgccggatt atgctgatcg
     6661 ctgacagtat tgttgctttg gcgaccattg ttctggctgt tttgttcgcg ctggatgtcg
     6721 ttgaagtctg gca
//
LOCUS       DBNGSX010000117         3744 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_147369, whole genome shotgun sequence.
ACCESSION   DBNGSX010000117 DBNGSX010000000
VERSION     DBNGSX010000117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_147369"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="AC16CB_03905"
     CDS             <1..294
                     /locus_tag="AC16CB_03905"
                     /inference="COORDINATES: protein motif:HMM:NF024919.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HIR3341646.1"
                     /translation="AYAHRPLSWAELKAEIAAGRPVIAWIIGSVNNGIPVYYTPSDGL
                     HTVVARYEHTVIVTGYDSNNVYYLNGATIYSRTISQFLDSWSALGNMAITAHP"
     gene            complement(278..1186)
                     /locus_tag="AC16CB_03910"
     CDS             complement(278..1186)
                     /locus_tag="AC16CB_03910"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR3341647.1"
                     /translation="MIEAVVFGNITLDIICKTVNDVPRHDSLAFEEAAVMPGGCGSNV
                     ALGLCALGVPTALVGCIGSDDAASVVKKFWGRFGLDHRFVQTVEGHSTGISIGLVDRD
                     MQPRFIHTPGANASLTVDVLDIPFLVQEGARFLHIAGYFVLPGILDGRLPKTLEKAQR
                     FGLVTTLDVVRSKRMQDPSLLWPCMPFLDIFLCNAYEARRLTGEKEPHHAASALRTRG
                     AKAVVVKLGKDGCWVESTDFNGHIDAPRVEIVDTTGAGDAFAAGLIRGLSAGDGLYDA
                     CVAANQAGARMVGYLGAVGGWIKDEQ"
     gene            complement(1291..1524)
                     /locus_tag="AC16CB_03915"
     CDS             complement(1291..1524)
                     /locus_tag="AC16CB_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341648.1"
                     /translation="MPRIRCHYMDCVLLDDGYCGAAAIELDPDMGCMTYKRSDDLVEE
                     DDWDAEEEEELDEWEELDGEDEDDELWIDDDEF"
     gene            complement(1526..1987)
                     /locus_tag="AC16CB_03920"
     CDS             complement(1526..1987)
                     /locus_tag="AC16CB_03920"
                     /inference="COORDINATES: protein motif:HMM:NF019499.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HIR3341649.1"
                     /translation="MITDSKLRSAAIVVSAIGAIDSLYLSWVKITHSQAFCGTSGQCE
                     TVNNSPYSEIAGIPIAYLGLGAYLVVIGLLYLEKREGLWRDNAPLLVFGISLVGVIYS
                     AYLTYLEIAVIHAICPYCVVSAIMMLFLLIISIIRLVQSQTTLNPVQNGGG"
     gene            complement(2009..3253)
                     /locus_tag="AC16CB_03925"
     CDS             complement(2009..3253)
                     /locus_tag="AC16CB_03925"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF015418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIR3341650.1"
                     /translation="MSDLLSVSEALQRLLSAFSSLEIETVSITKAVGRVNAGSVQATF
                     DIPPFDNSSMDGFAVRAEDISMSSSDNGVVLNVVADIPAGQVSDVLLGKGEAARIMTG
                     APLPAGADAVVPVEDTDFNDRQPGTAAPPKVLVYRAVNSGENVRPRGQDVAAGEIVLT
                     AGQRLRPQDIGFFAMLGIPHINVYRLPRIAIFSSGDELIPVSSNLAPGKIYDSNTYTL
                     IGQVEKYGAHPVNYGIVPDNIEAVRACLDRAVTEQVDLILSSAGVSVGAFDFVKHVVE
                     QGGSLGFWRVNMRPGKPLAFGEYKGIPYIGLPGNPVSAFVGFEVFVRPSILKMSGAAD
                     FTRPVTKVRILEAIESDGRESYLRGVVTHKLGGWEARLTGHQGSGNLRSLVQANALLL
                     VPSGVKSLPIGAEVDAWILDDL"
     gene            complement(3557..>3744)
                     /locus_tag="AC16CB_03930"
     CDS             complement(3557..>3744)
                     /locus_tag="AC16CB_03930"
                     /inference="COORDINATES: protein motif:HMM:NF024687.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR3341651.1"
                     /translation="DVATLIADEGVNMTGINVDVNRNLAILDLTLEVPDVAKLSRVLD
                     RLENLPNVMAAQRVKPG"
BASE COUNT         1065 a         1022 c          729 g          928 t
ORIGIN      
        1 gcttatgccc atcgcccatt gagttgggct gagctcaaag cagagatagc cgccggcagg
       61 ccagtgattg cttggattat cggatcggtc aataatggca tcccggtata ttacaccccc
      121 tccgatggtc tccataccgt tgttgcgcgc tacgaacaca ccgttattgt gaccggttac
      181 gattcaaata atgtgtatta ccttaatgga gcgacaatct attctcgaac gattagccaa
      241 ttccttgatt cgtggtctgc cctagggaat atggctatca ctgctcatcc ttgatccatc
      301 cccctaccgc acccaaataa cctaccattc gagctccggc ttgattggct gccacgcagg
      361 catcatacaa gccatcgcca gctgataaac ctcggatcag acctgcagcg aacgcatctc
      421 cagcgccggt ggtatcgact atttcgactc gtggagcatc aatatgcccg ttgaagtctg
      481 tgctctctac ccaacaacca tccttgccaa gctttacaac cactgcttta gcccctcgag
      541 ttcgtaaagc gcttgctgca tgatgaggtt ccttttcacc tgttaatcgg cgcgcttcat
      601 atgcattgca aagaaaaata tccagaaatg gcatacaagg ccagagcaaa ctgggatctt
      661 gcatccgctt agatctaacc acatccaacg tggtaactag cccaaaacgt tgtgcctttt
      721 ctaaagtctt tggcagtcgc ccatctaaga tccctggaag cacaaaataa ccagctatat
      781 gcagaaaccg agccccttcc tgaaccaaaa aaggaatatc gagcacatct acagtaagag
      841 acgcattcgc tccaggtgta tgaataaacc gcggttgcat atcccgatcg acaagaccta
      901 tgcttatgcc agttgagtga ccctccaccg tttggacaaa acgatggtcg aggccaaaac
      961 gcccccagaa tttctttacc acacttgccg catcgtcaga accaatacat cctactaagg
     1021 ctgttggcac gcccagcgca catagcccca aagcaacgtt tgaaccgcag ccccccggca
     1081 ttactgccgc ctcctcaaaa gccagagaat catgtctggg tacatcgtta actgttttgc
     1141 aaattatatc gagggttatg ttcccaaata caactgcttc aatcatctca aattaaatga
     1201 gccttccaat tgacagaagg ctccattaaa aataaaaagt atgtttaaca ttgcaaattt
     1261 caaggaaagc gcaccgacac gatagtcatc ctagaactcg tcatcgtcaa tccataattc
     1321 atcgtcttca tcttcgccat ctaattcttc ccattcatcc agctcttctt cctcctctgc
     1381 atcccaatca tcctcctcaa caaggtcatc cgagcgtttg tatgtcatac agcccatatc
     1441 cggatctaat tcgattgcag cagcaccgca gtatccatca tctaataaca cacaatccat
     1501 gtaatgacag cgaatacggg gcatttcaac ctcctccatt ttgaacaggg tttaatgtag
     1561 tctgagattg caccagacga attattgaga taattaacaa gaataacatc atgatcgccg
     1621 agacaacgca ataagggcaa atagcgtgaa tgacagcaat ctctaggtat gtaagatagg
     1681 cagaataaat aacaccaacc aggcttatcc caaacactag aaggggagca ttgtcacgcc
     1741 acaatccctc tcgtttctcc aaataaagca gtccaattac aaccaggtat gcacctaatc
     1801 caaggtaggc gataggtatg cctgctattt ccgaataggg actgttattc acggtctcac
     1861 attgcccaga tgtgccgcaa aaggcctggc tatgtgtgat ctttacccaa ctcaagtata
     1921 gggaatcaat agccccaatc gccgaaacaa caattgcagc tgagcgtaat ttgctatctg
     1981 tgatcatagt aatttgacgg tcacctctct agagatcatc caatatccag gcgtccactt
     2041 ctgcgccaat cggcagtgat tttaccccag atggcaccag aagcaaagcg tttgcttgca
     2101 ccaaagagcg cagatttccc gatccttggt ggcctgtcaa tcgcgcctcc cagccgccca
     2161 acttatgggt aaccactcct cggagataac tctcgcgacc atcggactca atcgcttcca
     2221 aaattcttac cttggttacg ggtcgagtaa aatcggcagc cccagacatt tttagaattg
     2281 acggtcgcac aaatacttcg aaccccacaa acgctgagac tggattacca ggaaggccga
     2341 tatatggaat ccccttatat tctccgaatg caagtggttt gccgggacgc atattgacac
     2401 gccagaaacc cagacttcct ccctgctcta cgacgtgctt tacaaaatca aatgcaccca
     2461 cgcttacccc agcagacgat agtatcagat ccacttgctc ggtcacagcg cggtccaggc
     2521 aagcccttac agcctcaata ttatcgggca caatcccata gtttaccgga tgggcaccat
     2581 atttctcaac ttgaccgatc aaggtgtatg tattcgaatc ataaatttta cccggcgcaa
     2641 ggttggagct gactggaatt aactcatctc ctgaagaaaa aatagcaatc ctgggtagac
     2701 gatacacatt aatatgcggt attcccaaca ttgcaaaaaa gccaatgtct tgaggtcgca
     2761 ggcgttgccc agccgttagt acaatttccc ctgcagcaac atcttgccct cttggtctta
     2821 cgttctctcc agaattaacc gctcgatata ccaatacttt aggaggcgct gcggtgccag
     2881 gctgccggtc gttgaaatca gtgtcctcca caggaaccac ggcatctgca cctgccggca
     2941 aaggcgcccc tgtcattatt ctggcggctt ctcccttgcc taataagaca tctgaaacct
     3001 gaccagctgg gatatctgcc acaacattca acaccactcc attatcagaa gagctcatcg
     3061 agatatcttc tgcccgaacg gcaaagccat ccatactgga gttatcaaaa ggagggatat
     3121 caaaagtcgc ttgaaccgac cccgcattca ccctcccaac cgcttttgtt atggagactg
     3181 tttcaatctc aagtgatgaa aaagccgaca aaagtctttg aagtgcttcg gagacactca
     3241 gcaaatcgct catacgatat aaaagaaatt ctttgactta cgcacattca tcgaatgggc
     3301 gcggcgcaaa attataccat tcccttgaat aaaaattgaa ctattgtgcc ttaataaaca
     3361 caaacatttt tattaggcaa actcatcaat tatccgcgag attgtagaga gcaaaataaa
     3421 gcattgctat aaacaaaata gaaggttatt ttaatgattt accagattta ggctaatata
     3481 cactgcgccc catttctagc ccccaaggca cataaagggt atgcacatca cgcaaattga
     3541 tatccttcgt gatgttttac ccaggtttga cacgctgggc agccatcaca ttgggtaaat
     3601 tttccaggcg atcgagtacc cggctcaatt tggctacgtc gggaacctcg agagttaaat
     3661 ccaaaattgc cagattcctg tttacatcta catttatgcc ggtcatattg accccttcat
     3721 cggcaatcaa tgtagccaca tcct
//
LOCUS       DBNGSX010000118        10245 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_562799, whole genome shotgun sequence.
ACCESSION   DBNGSX010000118 DBNGSX010000000
VERSION     DBNGSX010000118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10245)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10245)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10245
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_562799"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1880
                     /locus_tag="AC16CB_03935"
     CDS             <1..1880
                     /locus_tag="AC16CB_03935"
                     /inference="COORDINATES: protein motif:HMM:NF014071.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR3341652.1"
                     /translation="LVIEIIHGSTVATNALLERNGARTALITTRGFGDVIQIGRQERP
                     SLYDLNFVPAQPLVPEDLRFEVDERVNSSGNVQLALSVEQVKEISSYLKQHNPESIAI
                     CLLFSFLHPDHERRIAEQLRQEGYFVSLSCEVLPEFREYERTSTTVVNAYVSPVLDAY
                     LDQLETELRSISSSKLQIMQSNGGAISPYEARQKGVHCILSGPAGGVIGSQYIANQTE
                     VTNETSTGSEDSLKLITFDMGGTSTDVSLLVGEPNITTEATISGLPIRIPLLDIHTIG
                     AGGGSIAYVDAGGALRVGPQSAGADPGPACYGRGELPTVTDANLVLGRLDPDYFLGGK
                     MPLEAERSRQALHKLGLSLGLDSVNAALGVIEVANAHMERALRLISVERGYDPAEFTL
                     LSFGGAGGLHACMLAKKVGMHKIIIPPYASVLSALGMLAANVIKDYSLTVMLPGDTQI
                     DTIESAYAPMVERAMVELQKEGFSEDLIHIERNLDMRYKGQSYELIVPFSDRCLEEFQ
                     ALHHKEYGYFRAEAPIEIVNLRVRSIGKVVPPQLRLNKDAGPDPARAYIGSRPVLFHE
                     GSLPTPFYRGEELLPGNQIAGPAVIVRDDTTILLLDDVSGSVDTFGNLWLQIHSEEYG
                     Q"
     gene            1877..3496
                     /locus_tag="AC16CB_03940"
     CDS             1877..3496
                     /locus_tag="AC16CB_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR3341653.1"
                     /translation="MNPEVRVDPVRLEVFKHLYASIAEEMGVVLRKASYSPNIKERRD
                     FSCAIFDERGTMISQAAHIPVHLGSMPLSVRSAISAFTSASHPEGFRPGDMVILNDPY
                     RGGTHLPDITLIAPVFLQSTGGDENLMGYVASRAHHADVGGIAPGSMSIAREIFQEGI
                     VIPPIKLFDGGQINQSVLDLILSNVRTPQERLGDLWAQVAANQRGVTRFLEIINRYGV
                     HETRLYMQLLLAYSERMTRRLLEAIPDGEYLFEDYLDDDGINLEPVPIRVKITVSGDE
                     AIFDFSGSGKQVQGSLNAVYAITLSAVNYVVRCLVGLDVPNNGGCLAPVKLIAPRGTV
                     VNAEHPAPVAGGNVETSQRIVDVLLGALALACPGRVPAASQGTMNNITIGGWDPDQNN
                     LFTYYETVGGGMGARPTSDGPTAMHSHMTNTLNTPIEALEYAYPLQVVRYEIRRGSGG
                     QGEFNGGDGICRDIKLLADAQVSLISERRHIPPYGLAGGQPGLRGENVLIKGIEEYQL
                     PGKGTFDLKDGDILSIRTPGGGGYGDVEPLA"
     gene            complement(3488..3895)
                     /locus_tag="AC16CB_03945"
     CDS             complement(3488..3895)
                     /locus_tag="AC16CB_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341654.1"
                     /translation="MGRIINPDNVGSLRNRVSKAVVLALNEYSEKSYPRGEARDMLAF
                     VILSLEAISNTIDQSVLAWEKRGYWVKADRFRLEWLWAKQLAESLRKALLQDDWASAE
                     KIVYQIRQKLIDVQVSPRHRMGKPWVGAWQKLR"
     gene            4089..4170
                     /locus_tag="AC16CB_03950"
     tRNA            4089..4170
                     /locus_tag="AC16CB_03950"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4123..4125,aa:Leu,seq:tag)
     gene            4301..5749
                     /gene="tig"
                     /locus_tag="AC16CB_03955"
     CDS             4301..5749
                     /gene="tig"
                     /locus_tag="AC16CB_03955"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR3341655.1"
                     /translation="MKIDIQPREDHQVILTVEVEPQMLEDAKARAARHISKHTKIPGF
                     RPGKAPLAVVQRTVGAEAILEEAVELLINDVYPKALDESGIEPYGPGSLENVKSMTPP
                     VFEFIVPLRAEVELGDYQSIRLPYELSDIRDEEVENVISGLLEQQAVIEPVERKAQEG
                     DLIYLHLDAHRKQPKEGESQTIIDNRAIPVVVLKEDDDSSNDWPFPGFSRKLIDLSSG
                     EEVSFEYDFPEDAANESLQGVKADFHVSVDAVNSRTLPELNDEFAQSVGDFPTLDSLR
                     SQIRKNLEEQNLEMYHETYDEDLLEKVVEMSSIKYPPQMLEHEIDHLIQHLKERLERQ
                     RLDMDLYLKSRQMDLDGLREELKPLAEARIKKSLVLFDVADQLKINVSPDDLQAETER
                     TLGDFSRIMPEKEFRKMVASQEERSDLIGNIMMDMIIVRAKEQLRDIARGLEPKSSDQ
                     LQETDDTEKPAAEVESENASSETVDGEDEENN"
     gene            5820..6434
                     /locus_tag="AC16CB_03960"
     CDS             5820..6434
                     /locus_tag="AC16CB_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015130999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HIR3341656.1"
                     /translation="MVKNPLSLIPTVIETSGRGERYFDIYSLLLRNRIVFLGTPINDQ
                     ISNLIVAQLLYLSSEDPEAPIQMYINSPGGQVYAGMAIYDTMQMIPNPLSTLAVGMTA
                     SFGTILLTAGTKGQRYALPNATIHMHQPLGGAQGQASDIEIQAREILRLKDKLNEILA
                     KHTNQSVETIEKDTNRDFYLDAEGAVEYGLVDQVMELPNPKQSG"
     gene            6511..7383
                     /locus_tag="AC16CB_03965"
     CDS             6511..7383
                     /locus_tag="AC16CB_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018461587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HIR3341657.1"
                     /translation="MLHPSTFSIVMYDAKEKAWGIVVASKFLAVGSVVPWARANAGAV
                     ATQSFANTTYGNRGLTLMEAGHSAEETLALLLDDDPERELRQVGLVDAQGRSATYTGS
                     SCFVWAGGLSGDGFAIQGNILAGEQVILAMEASLRENTEKTIIWRLYNALLAGDQAGG
                     DRRGKQSAAILVVKAQAGYGGFNDRWVDYRVDDHHEPVKRLGELLNLHELYFGKSPLE
                     ERVRLVGEPLRQLQDIMAKFGYYRGRFHGEYDAPTQTALRAFIGNENFEDRTNFDAGL
                     IDRPVLEHLLSRFG"
     gene            7389..8183
                     /locus_tag="AC16CB_03970"
     CDS             7389..8183
                     /locus_tag="AC16CB_03970"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HIR3341658.1"
                     /translation="MKTPTSADIEALILDMDGVLWRDDQPIGNLKAIFSQIQQQGWQV
                     TLATNNATRSVEQYLKKLQGFGVSLNPDQIVNSAQAVVHYLAINHPLGGNVYVIGEEG
                     LLHTLAEYNFRHSLENPIAVIVGMDRELTYKKLRQATLLIRAGIPFIGTNPDRTFPTP
                     DGLVPGAGAILAALEASTSISPIIVGKPSPEMYMVAMDRMGIKPEATLVVGDRLETDI
                     AGAQKLGCPTALVLSGVTSWQAAQSWSPRPEWIEPDLASLLNRLVG"
     gene            8187..9230
                     /gene="rlmN"
                     /locus_tag="AC16CB_03975"
     CDS             8187..9230
                     /gene="rlmN"
                     /locus_tag="AC16CB_03975"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual-specificity RNA methyltransferase RlmN"
                     /protein_id="HIR3341659.1"
                     /translation="MNEREQQFIFDIDYEGLVSIIESWGEPLYRAQQIWQGLYKNLWN
                     EPDRFTTLSGKLRKKLAEVFTFYQIEPVQVLTSGDKETEKTLFRLSDQRAIEAVLMRY
                     NKRRTLCISTQSGCAMGCVFCATGQMGFQRNLSSGEIVEQVIYFARKLQAQDDKVSNV
                     VIMGMGEPFHNYDATMQAIERLNHPEGMGLGARRFTISTVGLVPAIKRFTAEHRQINL
                     AVSLHASDDDLRSSMLPINKKYPISELIQTCRDYVKQTRRRITFEWALIRDVNDSIDQ
                     ARQLAELLQGMLCHVNVIPLNPTQRYTGQATTRQRALAFKSELDKRGVPCSIRLRRGI
                     DIQAGCGQLAIEV"
     gene            9258..9476
                     /locus_tag="AC16CB_03980"
     CDS             9258..9476
                     /locus_tag="AC16CB_03980"
                     /inference="COORDINATES: protein motif:HMM:NF020940.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HIR3341660.1"
                     /translation="MSDQIYRLIYPVSLLSVPVINQLIRRYDITVNILRAHIGGENRW
                     MEVQLTGDPMVIEDAIHWLKDQGIEVTY"
     gene            9495..>10245
                     /locus_tag="AC16CB_03985"
     CDS             9495..>10245
                     /locus_tag="AC16CB_03985"
                     /inference="COORDINATES: protein motif:HMM:NF015413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR3341661.1"
                     /translation="MTENDAELNARRIRAALEIQRTDSAIAILYELRPADRADVFNML
                     SDTEQEILLPLLDIPATADLLEELEDQDALDAIGDFSPEKLGDVLDEMQPDEAADLLG
                     DLSPEQVTQALAHMEDADDDNPLLGYPDETAGGLMTTSFIALRRYTTAEQAIEFMRQV
                     ELETEVPYYLFVVDREKRLIGVIGLREIIVAQPSTTMETIMDREIVFVTVTTDQEEVA
                     RVISRYDLAAVPVVDESGRLIGVITHDDIVDV"
BASE COUNT         2752 a         2268 c         2574 g         2651 t
ORIGIN      
        1 atcttgttat cgagataatt catggctcga ctgttgctac aaacgcactt ctggagcgca
       61 atggagcacg caccgccctt atcacgacac gcggctttgg tgatgtaatc caaattggtc
      121 gccaggaacg tccttcccta tatgatttaa actttgtccc tgcccaacca cttgtgccgg
      181 aagatctgcg gtttgaagta gatgagcgtg tgaacagcag tggcaatgtc cagctagcat
      241 taagcgttga acaggttaag gaaatttctt cctatctaaa acaacataat cccgaatcga
      301 tagctatttg cctgctcttt tccttccttc atcctgatca tgagcggcga attgccgagc
      361 aattacgcca agaaggatat tttgtttcat tatcctgcga ggtattgccg gaattccgtg
      421 agtacgagcg aaccagtaca accgtggtta acgcctatgt atcgccagtc ttggatgctt
      481 atctcgatca attggaaacg gaattgcgct caatctcaag ttcaaaactg cagattatgc
      541 aatccaacgg cggtgcgatt agcccttatg aagctcggca aaagggtgtg cactgcatct
      601 tatctggacc tgctgggggt gttattgggt cacaatatat tgccaatcaa actgaggtaa
      661 caaacgaaac cagtactggt tcggaggatt cacttaaact gatcactttt gatatgggtg
      721 gcacctccac agatgtgtcg ttgctggttg gagagccaaa cattaccact gaagccacaa
      781 tcagcgggtt gcccattcgg atccctctat tggacatcca tacaatcggc gcgggaggtg
      841 gatctatagc atatgttgat gcgggaggtg cattgcgcgt cggtcctcaa agcgctggag
      901 ctgaccctgg tccagcttgt tatgggcgcg gtgagttacc aactgtgact gatgctaact
      961 tagttcttgg tcgtttggac ccagattatt tcttgggcgg taaaatgcca ttagaggccg
     1021 agcgatcaag acaagcgctt cataagcttg gcttatccct cggattggac agcgtgaatg
     1081 ctgcccttgg agtgattgaa gttgccaatg cgcatatgga acgcgctctg aggcttatct
     1141 ctgttgagcg tggttacgat cccgctgaat tcaccctgct ttcctttgga ggtgctggcg
     1201 gccttcatgc ctgtatgtta gccaaaaaag ttgggatgca caagattatc atccctccat
     1261 acgcatctgt actctctgcc ctgggcatgc tggctgccaa tgtaatcaaa gactactcat
     1321 tgactgtgat gttgcccggc gatactcaaa tagacacaat tgagagtgcc tatgctccta
     1381 tggtagaacg cgctatggtt gaattgcaga aggaaggttt ttcagaagat ctaattcata
     1441 tcgaacgcaa tctcgatatg cgctataagg ggcaatctta tgagttaatt gttccgtttt
     1501 cagatcgctg tcttgaagaa tttcaagctt tacatcacaa ggaatatggg tattttcgtg
     1561 cagaagcgcc aattgagatt gtcaatttgc gggtacgctc tattggcaag gtagtaccac
     1621 cgcaactgcg gttaaataaa gacgcaggcc cggacccggc acgtgcctat attgggtctc
     1681 gacctgtttt attccacgaa ggatcacttc caaccccctt ttatcgtgga gaggaattat
     1741 tgcctggaaa ccaaattgca gggccggctg ttattgtcag ggatgatacg accattttgc
     1801 ttttggacga tgttagcgga tcagtggaca catttggtaa cctttggctg caaattcaca
     1861 gcgaggaata cggacaatga acccagaggt gagggtagac cccgtacgct tagaggtctt
     1921 taaacacctc tacgcttcaa ttgcagagga gatgggggtc gtcctgcgta aagctagcta
     1981 ctcccccaat atcaaagaac gccgcgattt ctcctgcgcg atattcgacg aaagagggac
     2041 catgatctcg caagcggctc acatacctgt ccatttgggt tctatgcctc tctcggtcag
     2101 atcagcgata agcgctttta catctgcttc ccaccctgaa ggattccgcc ctggcgatat
     2161 ggtcattctt aatgatccct atcgtggcgg gacacacttg ccggacatca ccctcatcgc
     2221 accagtgttt ctgcagtcaa ctggaggaga tgaaaatctc atgggatatg tcgccagccg
     2281 tgcccaccat gctgatgtag gcggcatagc gccaggctca atgtccattg cacgggagat
     2341 attccaagaa gggattgtta tcccgcctat aaagctgttt gatggtgggc aaattaacca
     2401 atctgtactc gacttgatcc tttcgaatgt gcgcacgcct caagagcgtc tgggcgattt
     2461 atgggcgcag gttgccgcta accaaagggg cgttacccgt ttcttagaga taatcaatcg
     2521 ctacggtgtt catgaaactc gcttgtatat gcaattatta ctggcatact ccgagaggat
     2581 gacccgtcgt ctattggagg ctatcccaga tggtgaatat ctattcgaag attatttgga
     2641 cgacgatggg ataaatttgg agccagtgcc aattcgtgtg aaaatcaccg tctctggcga
     2701 cgaagcgatc tttgattttt cagggtctgg aaaacaggtt cagggcagcc tcaacgcagt
     2761 ctatgcaatc actttatcgg ctgtcaatta tgtagtgcgc tgtttggttg gtttagatgt
     2821 gcccaataac ggtggttgcc ttgcccctgt aaagttgatt gcccctagag ggacagtggt
     2881 gaatgcagaa catcctgctc cagtggcagg aggaaacgta gaaacctctc agcgcatcgt
     2941 cgatgtactc ctgggggcgc tggcattggc ttgccctgga cgtgttccag ctgccagcca
     3001 gggcacaatg aataacatca ccattggggg gtgggatcca gaccagaata atctatttac
     3061 ttattacgag acggttggag gaggcatggg ggcgcgcccg accagcgatg gtcccaccgc
     3121 gatgcatagt cacatgacga acacgcttaa taccccgatt gaagccttgg aatatgccta
     3181 cccactacaa gttgttcgct acgaaattag gagaggatcg gggggtcaag gtgaatttaa
     3241 tggcggcgat ggtatttgta gggatatcaa actgctggca gacgcccagg ttagtctaat
     3301 tagcgagcgc aggcacatcc ctccctatgg tcttgctggg gggcaaccag ggttgcgcgg
     3361 agagaatgtc ttgattaagg ggattgaaga atatcagcta ccagggaaag ggacatttga
     3421 tttgaaagac ggggatattt tgagtatccg cacgccaggc ggtggaggct acggtgatgt
     3481 cgagccctta gcgtaacttc tgccacgcac caacccaggg cttgcccatg cgatggcgag
     3541 gagaaacctg tacatctatc aatttttgac gaatctggta gacaatcttt tcagcacttg
     3601 cccaatcatc ttgcaataat gcttttcgca aactctccgc cagctgtttt gcccagagcc
     3661 attccaaacg aaaacggtct gccttcaccc aataaccgcg tttttcccat gctaaaaccg
     3721 attggtcgat agtgttagag atggcttcaa gggaaagtat gacaaaggcg agcatgtcgc
     3781 gcgcctcccc cctaggatag ctcttctcag aatattcatt tagagccaag acaactgcct
     3841 tggacaccct attgcgaagc gaaccgacat tatctgggtt gattatacgt cccaagtgtt
     3901 tctcctcgat ataaatagtt ctccaagttg attatttgac agtcctctga gaaaattaaa
     3961 ccctttccaa ggtacaatta gttactcatt atagcacctt gagcgttcga tattttataa
     4021 accaattatt gacgatgaaa cctcctgagg atataatccc cgagcaacta aacggaggca
     4081 acgtcgttgg ggaagtgctg gaattggcag acaggcttga cttaggatca agtgccgcaa
     4141 ggcgtgaggg ttcaagtccc tccttcccca cttatcgcca atggagagcc cgcttttaaa
     4201 atacgaaggc gggcttcttc atatattttt ctaacaacta ctaaatgttt cgcatgatat
     4261 aatcctgccg tctttagcgt ttactgaaag gaatttcaac ttgaaaattg atatccaacc
     4321 tcgtgaagat catcaggtca tattgactgt agaagtcgag ccccaaatgt tagaggatgc
     4381 caaggcgcga gctgcccgcc acatctctaa gcacacgaaa atccctggtt tccgccctgg
     4441 caaagcccct cttgccgtag ttcaaaggac tgtcggtgca gaggctattt tagaggaagc
     4501 cgttgaatta ttaattaatg atgtctatcc gaaagccctc gatgagagtg gtattgaacc
     4561 atatggacct ggctcgttgg aaaatgtcaa gagcatgacc ccaccagtat ttgaatttat
     4621 tgtgccttta agagcagagg tcgagctggg cgattatcag tccattcggt tgccgtatga
     4681 gttgagtgat atccgtgacg aggaagtgga gaatgtaata tccggtttgc tcgaacagca
     4741 agctgttatt gaaccagtcg agagaaaggc acaagaagga gatcttatat atcttcacct
     4801 tgatgcccat cgtaagcaac caaaggaagg cgagtcacaa actatcattg ataatagagc
     4861 tatcccagtc gtggttctaa aagaagatga cgatagcagt aatgattggc catttcctgg
     4921 tttctcccgc aaattgatcg atttatcttc tggcgaggag gtcagctttg agtatgactt
     4981 ccctgaagat gctgccaatg aatcgttgca aggtgttaaa gccgatttcc atgtgtctgt
     5041 cgatgcggta aactcgagaa ctttacctga actaaacgat gaatttgctc aatcggtggg
     5101 cgattttccc actttagatt ctctgcgttc ccagattcgc aagaatcttg aagaacagaa
     5161 ccttgagatg tatcatgaga cctatgatga ggatctgctc gaaaaagtcg ttgaaatgtc
     5221 gagcattaaa tatccgccac agatgctcga acacgagatc gatcacttga tccaacacct
     5281 caaagaacga ttggagcgac agcgtttgga catggacctt tatctaaaat ctcgtcaaat
     5341 ggatttagat ggtcttagag aagagttaaa acctttggct gaagctcgaa tcaagaaatc
     5401 actggtcctg tttgatgttg ccgatcaatt gaaaattaat gtcagtccag atgatttgca
     5461 agcggaaacc gaacgtactt taggtgattt ttcccgcatt atgcccgaaa aagaatttcg
     5521 taaaatggtc gcttcgcaag aggaaagatc agacctgatt ggcaatatca tgatggatat
     5581 gattatcgtg cgtgccaaag aacaactgcg tgatatcgct cgtggattag agcctaaatc
     5641 tagtgatcaa cttcaggaaa ctgatgatac tgaaaaacct gccgccgagg tagaaagtga
     5701 aaatgcttct tcggaaacgg tggacggaga agacgaagag aataactagc cccataattc
     5761 tggggacaaa tagtgatttg atcgcataat tttgtgtatc gaagagaggc aaatagtcta
     5821 tggttaaaaa tcccctttcc ttaatcccta cggtaataga aacctcggga cgcggtgaac
     5881 gatattttga tatctattcg ttattgttgc ggaatcgcat cgtatttctc ggcacaccta
     5941 tcaatgatca gatatcaaat ttaattgtcg cccaactgct gtacctgagc agcgaagacc
     6001 cagaggctcc aatacagatg tatattaact cgcctggagg gcaggtatat gctgggatgg
     6061 caatatatga tacgatgcaa atgataccta acccactaag tacccttgca gtcggcatga
     6121 cagcttcctt tggaacgatt ctcctaactg cagggacaaa aggtcaacga tacgcgctcc
     6181 caaatgccac aatccacatg catcagccgt taggtggcgc ccagggacaa gcgtcagata
     6241 tcgaaataca agcacgcgaa atccttcgcc tgaaggacaa gttaaacgaa attttagcca
     6301 agcataccaa tcagagtgtt gagacaatcg agaaagatac gaacagagat ttctatctcg
     6361 atgccgaggg agctgttgag tacggtttgg tggatcaggt tatggagcta cctaacccca
     6421 aacaaagtgg gtaaaacgct ctgaccactg agatcattcc ccctccatgc aaattgctgc
     6481 atggaggggg aatattatca ggaggtattt atgcttcatc cgtcgacatt ctctatcgtg
     6541 atgtacgatg cgaaagaaaa agcctggggg atagtagtgg catcaaaatt cctggctgtg
     6601 ggttcggtag tgccttgggc tcgtgctaat gcaggtgcag ttgctacaca atctttcgcg
     6661 aacaccactt atgggaatcg aggattgact ttgatggaag cagggcattc tgctgaagaa
     6721 accctagcgt tactattgga tgatgacccc gagcgagaat tgcgacaggt tgggttagtt
     6781 gatgcccaag ggagatcagc tacatatact gggagctcct gttttgtgtg ggcgggcggc
     6841 ctgagtggtg acggtttcgc gattcaaggt aatatactcg ccggagaaca ggttattctt
     6901 gcaatggaag catcattaag ggaaaacaca gagaaaacga ttatatggcg cttgtataat
     6961 gctctgctag ccggcgacca ggcgggagga gatcgacgtg gcaaacaaag cgcagccatt
     7021 ctggtggtaa aagctcaagc tggctatggt ggcttcaatg atcgttgggt ggattatcgt
     7081 gtggacgacc accatgaacc tgtcaagaga ctgggtgagc ttttaaatct gcatgaactt
     7141 tactttggaa aaagtccctt agaggagcgt gttcgcctgg ttggcgaacc cctgcgccag
     7201 cttcaagata ttatggcgaa attcgggtat taccggggtc gtttccacgg agaatacgac
     7261 gctccaaccc agaccgctct gagagcattt attggcaatg aaaactttga agaccgcaca
     7321 aatttcgatg caggattgat cgaccgacct gtcttggaac acctgttaag ccggtttgga
     7381 taaactctat gaaaacaccc acatccgctg atatcgaagc gctgatcctg gacatggatg
     7441 gagtattgtg gcgggatgat cagcctattg gcaatttgaa ggcaatattt tcccaaatcc
     7501 aacagcaagg atggcaggta actctcgcaa cgaacaacgc aacacgctca gtagaacaat
     7561 atcttaagaa acttcaaggt tttggcgtat cactcaaccc agatcaaata gtcaattcag
     7621 cccaagcggt ggttcattac ttagccataa atcacccctt gggtggcaat gtgtatgtca
     7681 tcggagaaga ggggttgctg catactctgg ctgagtataa ttttcgacat agccttgaaa
     7741 acccaatagc ggtgatcgta ggaatggatc gggaattgac atataaaaag ctgcgccaag
     7801 caactttgtt gatacgggca ggcatcccat ttatcggtac aaacccggat cgcacattcc
     7861 ccactcctga tggtctggtt ccgggagcag gcgcaattct tgccgctctg gaagcttcta
     7921 catctatctc gcccattatt gtcggcaaac cctcgcctga aatgtacatg gtagcaatgg
     7981 atagaatggg gataaaacca gaagctacat tggttgtcgg tgatcgtctg gaaactgata
     8041 ttgcaggagc gcagaaactt ggttgtccaa cagcactggt gctatctggg gtaacaagct
     8101 ggcaagcggc tcaatcctgg agcccacgtc cggagtggat tgaaccagat ctggcttctt
     8161 tattgaaccg tttagtagga taaaaaatga atgagcgaga acagcagttc atatttgata
     8221 tcgactacga aggtttagta tctataattg aatcatgggg agagcctcta taccgggcgc
     8281 aacaaatctg gcagggcttg tataaaaatc tctggaatga accagatcgt tttacgacat
     8341 tgtctggcaa gctgcgcaag aagctggctg aagtgtttac attttaccaa atagagccgg
     8401 ttcaagttct tacttctggg gataaagaga ctgagaagac gctctttcgt ctttctgatc
     8461 agcgcgcaat tgaggcggta ttgatgcgct ataataagcg tcggacgctg tgtatatcta
     8521 ctcagtcggg ttgcgctatg ggatgtgttt tttgcgccac tggacagatg ggatttcaac
     8581 gcaatttaag tagtggcgag atcgttgaac aagtcattta tttcgctcgg aaattacagg
     8641 cacaagatga taaagtgtcc aatgtggtca tcatgggtat gggtgaacct tttcataact
     8701 atgatgcaac gatgcaagcg attgaacggt tgaatcaccc tgaagggatg gggttgggtg
     8761 cgcgccgatt tacaatttcg acggtaggct tggtcccagc cataaagcgc ttcactgctg
     8821 aacacaggca gatcaatttg gcagtatcat tacacgcctc cgatgatgac cttcgctctt
     8881 cgatgttgcc aatcaataag aaatatccga taagcgaatt gatccagacc tgtcgagatt
     8941 atgtgaagca aacccgtcga aggatcacgt tcgagtgggc gttgatcagg gatgttaatg
     9001 atagtattga ccaggcaagg caactggctg aattactgca aggaatgtta tgtcacgtga
     9061 atgtgatacc actaaatcca actcagaggt atacaggtca agcgacgact cgccaacgcg
     9121 cccttgcctt taagtcagag ctggacaaac gtggggttcc ctgctccatt cgcctgcgga
     9181 ggggcattga tattcaagca gggtgcgggc aattagcgat agaagtatag gcaagtttca
     9241 aaagtttgga ggttgctatg tccgatcaga tttaccgact catctatcca gtttctttac
     9301 tgagtgtccc ggtaatcaat cagctcatcc gtcgctacga cataaccgtc aatatattac
     9361 gtgcacatat tggaggggaa aaccgctgga tggaagttca attaaccggt gatcctatgg
     9421 ttatcgaaga tgctatccat tggctgaaag atcaggggat cgaagttaca tactaatttc
     9481 gagtataaaa aatcatgact gaaaacgatg ccgaattaaa cgctcgacgg attcgagctg
     9541 cgcttgagat tcaacgaact gacagcgcaa tagcaatcct ctacgagttg cgccctgcag
     9601 atcgagccga tgtcttcaat atgctgagtg atacagagca ggagatcttg ctgcctttgt
     9661 tggatatccc ggcaactgca gaccttttgg aagagctaga ggatcaggat gctctggatg
     9721 caattgggga tttttcacca gagaaactgg gtgatgttct agatgaaatg cagcctgatg
     9781 aggcagcaga tttattaggg gatctttcac cagaacaggt cacccaagca ctggcgcaca
     9841 tggaagatgc cgatgatgat aacccattgc tcggctatcc tgatgaaacg gccggtggtt
     9901 tgatgaccac ttcatttatc gccttgcgaa ggtatacaac tgctgagcag gcgattgaat
     9961 ttatgcgtca ggtggagctg gaaactgaag tcccgtacta cctgttcgtt gttgatagag
    10021 aaaaaaggct gattggagtg attggcttac gcgaaatcat agttgcgcaa ccatctacta
    10081 cgatggaaac aatcatggat cgtgaaattg tattcgttac tgtgaccact gaccaagagg
    10141 aagtcgcccg agtaatatct cgttatgatc tggctgctgt gccagtggtg gacgaatcag
    10201 gacgtttgat aggtgtgatc acgcacgatg atatcgttga tgtgc
//
LOCUS       DBNGSX010000119         2895 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_285617, whole genome shotgun sequence.
ACCESSION   DBNGSX010000119 DBNGSX010000000
VERSION     DBNGSX010000119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_285617"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1850)
                     /locus_tag="AC16CB_03990"
     CDS             complement(<1..1850)
                     /locus_tag="AC16CB_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3341662.1"
                     /translation="MRRSGVLETHRITQNLDNTGSHPTQQLEPGSSLGNRYLIQDVIG
                     LGGMGAVYRARDLHFPNVEKLVAVKEMINQARDALVRSTIVRNFEREANLLATLDHPS
                     IPRIYDYFTHNERSYLILEFIKGKDLEAILGETDGFVPQEKVIYWAVELCDVLHYLHS
                     HKPEPIIFRDMKPSNVMINHHNHVILIDFGIAKPFQGSQRGTMIGTEGYSPPEQYRGE
                     ADHLADIYALGATLHHLLTKIDPRLEAPFSFGERPLRQFNPNLSPELEGVVYKALQYA
                     PQERYQSALEMKEALLIFIKNTGILPPKPDKKSVPGAQEQEFQPMWEFACEDEIRGSA
                     THANGAIYIGSYDNNLYALKATSGEFLWKYPADGGIVSKPLVYEGNIYFGSEDHRLHV
                     INARHGQINWTYFTNGPVRSSPYIAEGHVFIGSDDGYMHAVNTLSGRPAWKLDVGAAV
                     RSTPIVEQEYVYFGTEAGELFCVDFSSKVRWRFKAKRAITSSPIVQNGVVYFTSMDST
                     LYAVDAKTGWVIWRFRLGKASISTPCIAEDYIFTGAADGNIHCVDMRTAKQVWSFATE
                     HQVTGSPIIYNDSLYCGSVDKHVYCLEYSTGRLKWKYPTNGPITGTPIAV"
     gene            2028..>2895
                     /locus_tag="AC16CB_03995"
     CDS             2028..>2895
                     /locus_tag="AC16CB_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HIR3341663.1"
                     /translation="MINYENYLSPFTWRYGSPEMRRLWSEITKRLLWRRMWLALAEVQ
                     SEFGLLSPEQLDDLRTHVNDVDVARALEIEAEIHHDLMAELKTYAQQCPKGGGMLHLG
                     ATSTDIEDNADALRIRQSLDLLIENLKGLMEGFAEKIETWADTPLIAFTHLQPAEPST
                     LGYRLALYAQDLLTDWENMRRARAHLRGKGFKGAVGTGASYAELIGVEQLSEFETRLS
                     AHLGLPFFPVTSQTYPRKQDYQVISALAGLGGSLYKFAFDLRVLQSPSIGEMSEHFAE
                     KQVGSSAMPFKRN"
BASE COUNT          709 a          692 c          749 g          745 t
ORIGIN      
        1 tcgacagcaa ttggcgttcc ggtaattggt ccgtttgtgg ggtacttcca ctttagccgc
       61 cctgtgctgt attcaaggca ataaacatgc ttgtcaactg atccacaata caacgaatcg
      121 ttatagatga tgggagagcc agtgacctga tgctccgttg caaaactcca tacttgcttg
      181 gctgtgcgca tgtccacgca gtggatattt ccatctgctg cgccggtaaa gatgtaatct
      241 tcagcaatgc aaggggttga tatggatgct ttccctaagc ggaaacgcca aatcacccag
      301 ccagtcttgg catccactgc gtacagggtt gagtccatgg atgtgaagta aactacacca
      361 ttttgcacaa tgggcgagga ggttatggcg cgtttcgctt taaatcgcca acgcaccttg
      421 ctgctgaaat ccacacaaaa aagttcgcct gcttctgttc caaagtagac atattcttgc
      481 tctacaatgg gagtcgagcg cacagcggca cccacatcga gcttccaagc aggccgaccg
      541 ctcaacgtgt tgacggcatg catatagcca tcatccgatc cgataaaaac atggccttcg
      601 gcgatgtatg gcgaggagcg aactggccca ttggtgaagt atgtccagtt gatttgcccg
      661 tggcgggcat tgattacatg caagcggtgg tcttcagagc caaagtaaat attgccttca
      721 taaactaggg gtttgctgac aatgcctcca tctgcagggt atttccatag aaattcgcca
      781 gatgtagctt tcaaggcata taggttgttg tcgtatgaac caatgtagat agcgccattt
      841 gcatgggttg ctgagccgcg gatctcgtct tcacaggcga attcccacat cggctgaaat
      901 tcttgttcct gggcgccagg cacagacttt ttgtcgggtt ttggaggaag tatgccggtg
      961 tttttaataa atataagcag agcttccttc atttccaggg cgctctgata gcgttcttgc
     1021 ggcgcgtatt gtaacgcttt gtaaaccaca ccctccagct ctggagagag gttggggtta
     1081 aattgccgga gcgggcgttc accgaaggaa aatggcgctt caaggcgagg atcgatttta
     1141 gtcagaagat ggtggagcgt ggcacccaat gcataaatgt ctgctaggtg gtcggcttca
     1201 ccccggtact gttcgggcgg tgaatagcct tcagtgccga tcatggtgcc ccgctgactc
     1261 ccttggaaag gcttagcgat gccaaaatcg atcaggatga catggttgtg atggttgatc
     1321 atcacattgg agggtttcat gtcccgaaag atgattggct caggcttatg gctatgtagg
     1381 tagtgcaaaa catcgcataa ttctacagcc caatagatga ccttttcctg cgggacaaaa
     1441 ccatccgttt cgcctaggat ggcttccaag tctttcccct tgataaattc gagaattaga
     1501 taagagcgct cgttatgagt aaagtagtcg tagattctag ggatacttgg atggtctagg
     1561 gtggcgagca gattagcctc tcgctcaaaa ttgcgcacta tagtgctgcg gaccagagca
     1621 tcacgcgcct gattgatcat ttcctttact gcaactaatt tctctacatt gggaaagtgc
     1681 aggtcacggg cgcggtaaac tgctcccatg cctcccaaac cgatcacatc ctggatcaaa
     1741 tagcgatttc ccagcgatga gccgggttca agttgttggg tagggtgact gccggtgttg
     1801 tctaaatttt gagtaattcg atgagtttcc agaacaccac tcctgcgcaa taattttacc
     1861 aaccgcttga caaattatac aggatcattg ggctttgcct ttgtaagggc tggagatggg
     1921 gtgtgatcga tctttacctg cctacacctc ccataagttt actccactaa ggagattctc
     1981 aatgacccat tatacagtat tataactcgt gctaaaattt aaatgtgatg atcaattacg
     2041 aaaactatct ttcgcccttt acctggcgtt atggcagccc cgaaatgcgc cgcttgtgga
     2101 gcgaaattac aaagcggctt ctctggcggc ggatgtggct tgcgctagca gaagtgcaaa
     2161 gtgagttcgg cctattgagc cccgagcagt tggacgacct gcgcacgcat gtaaacgacg
     2221 tagatgttgc gcgagcatta gagattgagg cggagattca tcacgatttg atggctgagc
     2281 ttaaaaccta cgcccagcaa tgccctaaag gtggcggtat gttgcattta ggtgccacct
     2341 cgacagatat cgaggacaac gcggatgcct tgagaatacg ccagtcactt gacctgttga
     2401 ttgaaaacct gaaaggcttg atggaaggct ttgcagagaa aatcgaaacc tgggcagata
     2461 cgccattgat cgcatttact cacctgcagc cagctgagcc atctacattg ggatatcgcc
     2521 tggctttgta cgctcaagat ctattgaccg attgggagaa tatgcgccgc gcccgagctc
     2581 acctgcgagg caagggcttt aaaggagcag ttggcacggg ggcttcctac gctgagctga
     2641 tcggtgtgga acaactgtca gaatttgaga cacggctctc agcgcacctg ggcttgccct
     2701 tttttcctgt tacatcacag acatatccgc gcaaacagga ttaccaggtg atcagcgcgc
     2761 tcgccggact gggtggatct ctgtataaat ttgcttttga cctgcgagtg ttgcaatccc
     2821 cctcaatcgg cgaaatgagc gaacactttg ccgaaaagca ggtgggttca tcagccatgc
     2881 cattcaagcg caacc
//
LOCUS       DBNGSX010000120        14637 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_424444, whole genome shotgun sequence.
ACCESSION   DBNGSX010000120 DBNGSX010000000
VERSION     DBNGSX010000120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14637)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14637)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14637
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_424444"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="AC16CB_04000"
     CDS             <1..461
                     /locus_tag="AC16CB_04000"
                     /EC_number="2.3.1.-"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF046470.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HIR3341664.1"
                     /translation="LSLQAQAKPIAASDENGEAKGPVCFEVPSNYEITVNGKKLLGSA
                     QARKQGGILQHGTLPLWGDLTRITQALHYESESQRERSAARLQERATTVEEILGHRPT
                     WEAATRAFISAFKQELALELEPAELSAAEQGRAMELVSEKYGNPIWTERI"
     gene            complement(525..1223)
                     /locus_tag="AC16CB_04005"
     CDS             complement(525..1223)
                     /locus_tag="AC16CB_04005"
                     /inference="COORDINATES: protein motif:HMM:NF025380.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HIR3341665.1"
                     /translation="MANDNNLRGWVLVIGVLLILGLAAFGVVSVVQQSANQAKQAIQP
                     VNELTSNVATQVAQFLNPTPTVLPDPVTIIQGVRSLSRLETIQYSVEKVITAETNQGP
                     FGFLFGDRLILVAHGIVIAGVDLEKLTPENMQLRGGVLYVQLPEAEIFVATLDNDKSY
                     IYNRDTGLLTKGNINLETEARRAAEDEIELATLEDGILEQANQNAENYLYRLLRDLGY
                     PEVIFIRITPEPQD"
     gene            1297..1581
                     /locus_tag="AC16CB_04010"
     CDS             1297..1581
                     /locus_tag="AC16CB_04010"
                     /inference="COORDINATES: protein motif:HMM:NF016351.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HIR3341666.1"
                     /translation="MTLGIITLHLQIPGCTSLKEKRRRLKPLLARLHRQFNISVAEMD
                     QHDAWQESLIACAVVSNDIGFTTRSLQAIPDWVEVHWPDVVVLDNQIEVF"
     gene            complement(1722..2324)
                     /locus_tag="AC16CB_04015"
     CDS             complement(1722..2324)
                     /locus_tag="AC16CB_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3341667.1"
                     /translation="MSLPALIILVGLAYIVLFGGLSLLRREGLSSRFAIEALVITFMV
                     GGLTALTGIQIHPIMFLIVLYLITMRVRLLVDIANFFAQRSQFERANLFYNLAQRLWP
                     DQTGHLVIQVNQGTALLQNDALDEAIAVFTDVLKNANQGYLGVKYEAATHYNLGIAYR
                     RKDMEPQARLEFTAVLDTWPASQYAQRAEIALSKDRKKSN"
     gene            2616..4412
                     /locus_tag="AC16CB_04020"
     CDS             2616..4412
                     /locus_tag="AC16CB_04020"
                     /inference="COORDINATES: protein motif:HMM:NF014598.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HIR3341668.1"
                     /translation="MNAVLTLVLGLVGIGIGYGFYARTINAGVVKPDDKKATPARMFM
                     DGVDFTPANRNVLFGYQFKSIAALGPIVGPIVAIQWGWLPALLWVIFGTFFIGWVQDY
                     SAIIMGVREEGQSFGALSYRFVSPRARTILLMFIYFYLLLIMGAFGKIVGYDLMTNPA
                     VPLGVLLVIGVGLLAGQMTYKWKMDIILTSVVTVVLAFLGIWLGTLPGVKSLFLAING
                     GEKSPVVFGSVTQAQFIWSLVVLVICYFGAVLPIWRWAQPINYVAFWIVFLGIVAGTI
                     GLLVWHPSFPSDFPAFTNWAGNLCQTADCTGVEKAPLALGPLWPILFVTIACGAVSGW
                     HSLVSSSGTARQLEKETDALYVGGGAMFLEMFLATLSILTAVVGVGVTRGFVGTADKP
                     GYVLLVAAGKNAGIFATGLSEMMARVGVPTSFGLPYGSVFLTLMALTIMYLVVRFMRV
                     ASAEFLGDSVSIFRNVTFGSIVALALSALLIWTGFWARIWVLFGGANQLMAALALLLV
                     TLWLMSNGKKYMWTLVPFLFMFVTTIAALIITAYNVFAQIARTPDMPIDGLIGNVIAG
                     VIAIFLVIAALILAWDALKAFQRIRATAPAKA"
     gene            4534..4845
                     /locus_tag="AC16CB_04025"
     CDS             4534..4845
                     /locus_tag="AC16CB_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341669.1"
                     /translation="MSCEMLLARANLDKAPYERGLDEYPPEWQEEFQRLASFVMDLAV
                     QYGDQIQIRIWDPRSLQGLAKSLRYGVRRYPTFVVDRRVKITGWDVSGLEQALLAAFA
                     A"
     gene            4948..5214
                     /locus_tag="AC16CB_04030"
     CDS             4948..5214
                     /locus_tag="AC16CB_04030"
                     /inference="COORDINATES: protein motif:HMM:NF052803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7990 family protein"
                     /protein_id="HIR3341670.1"
                     /translation="MIRQIARTTGDIFYGATIYEMVRDLKKERSHLEHLFILIVFGDL
                     LGVPVLPPYYTLRLLPYVMPDITGWKHSLLRERDLTDMCDVEIT"
     gene            5282..5551
                     /locus_tag="AC16CB_04035"
     CDS             5282..5551
                     /locus_tag="AC16CB_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018032434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7990 family protein"
                     /protein_id="HIR3341671.1"
                     /translation="MADENKKPSFLDGFKSVLYGMAGHDMARYALKTRGSMEHLFILI
                     TMGDLLGIPILPPYYSLRLLPYVVPQISTWKRRMLREKDLTDALA"
     gene            5686..6666
                     /locus_tag="AC16CB_04040"
     CDS             5686..6666
                     /locus_tag="AC16CB_04040"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HIR3341672.1"
                     /translation="MSLREVFEKNPERRYIMFGGKGGLGKTTFSAATAFWLAKQGYKV
                     LVFSVDPQASLSDIFQRDIFGKGPVEIMPNLYAQEIDADRRIHEYQEEIRQKIREMYG
                     LEQIPEEIENYIQAAAAEPAMEESAIFDEVVDIVVGGQYDFYIYDLVPLGHALYYLSM
                     ASVYDEWIDKITKLREEMAEYDQVAAVMKREKESEEDMILKELQYIKYRINTSSSILT
                     DKQKTAFFFVVTPEEMIILDTQKAAGLFAKFDVPIAGYIVNRVIPPELTKQKIPDYLR
                     NRLTMQKDYLTEIDQVFGGQVLARVPEFERDITGLTMIEQMADAMFGGGA"
     gene            6663..7643
                     /locus_tag="AC16CB_04045"
     CDS             6663..7643
                     /locus_tag="AC16CB_04045"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014433.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HIR3341673.1"
                     /translation="MIEKSMVQFLSDNPRLKYIFFGGKGGVGKTVLAGTAALWAAQQG
                     KKTLLASTNPVHSLSSLLDQNVFGKPTPVSGAPNLLAYEIDTRETIEKSKKEIREKIE
                     WFLKFADIKTKADEFVESATMNPAFEESAMFENMIDLMFEDKYQVYVFDTAPTANARR
                     LLGMSSVYSMWVNKMVQSREEAMSLKDLLSYSKKKKEKDPLMDYLLNLRERMGRAKML
                     LTDAELTAFFFITLPEALPIAVITRFINWFHDFGIPVGGVIVNMQIDKTSLGPDAPDF
                     VKNRVAMQDEHMLTIWKEFDGQVRAILPLLETEVRGVDMLKRMASLLYAT"
     gene            complement(7729..8373)
                     /locus_tag="AC16CB_04050"
     CDS             complement(7729..8373)
                     /locus_tag="AC16CB_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341674.1"
                     /translation="MNVGQWIVIGLCIFIGLWYATGFYYNRRKSKAVAAWLYTGLSLY
                     GNVKVSRLKGTAAWGAQFHVDCREAPFRYFEVAFLLEARENLPLWIYNRLLGTRDEIV
                     LKSNLLRVPSQELEAAYKGDQQFKNLVDGEEKRPYESMSVPPWFVLAYRGRKDLDSLD
                     HLQELLKKYNRGITRVSIQRKDPHLLMRANLSALQVEPAEKFLGEIRDFAISLR"
     gene            8461..9252
                     /locus_tag="AC16CB_04055"
     CDS             8461..9252
                     /locus_tag="AC16CB_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012407398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3341675.1"
                     /translation="MDLGLTNKRALVTGSSRGLGYETARLLAMDGCRVVINSRSSENL
                     FTAAKKLEEETGGQVFPLVGDVTDPAIPEQLVRKSAEALGGLDLLVTNSGGPPSGKFE
                     IFDDATWQKAVELNFLSHVRLIRAALPYLRQSKAASVLAITSYSVKQPIPNLVLSNSV
                     RAATVGLIKTLALELGGDGIRFNSILPAWTETERVIELMAFRAQTNGTTVEEEIAKQA
                     KDSPIGRMGHPEEFANAAVFLLSPAASYITGVMLTVDGGMFKGTL"
     gene            9356..11731
                     /locus_tag="AC16CB_04060"
     CDS             9356..11731
                     /locus_tag="AC16CB_04060"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HIR3341676.1"
                     /translation="MSAILNIAGSIARQGLTWLGRRRLPQITGNIDIPGLHAPAEIIR
                     DRWGIPHLYANDRHDLFFAQGFVHAQDRLFQMELNRRTSSGCLSEIFGPLALDTDRAS
                     RTFGFARLGRIDWENANDELRSVILAYTEGVNAYLNHPDSKLPVEFTLLGYHPELWKP
                     EDSSALARFMMWQLSHAWYGEIVRAQLIQAVGAERAAELEIHIPSQNPITLPRGIEFN
                     RLQPGEGLQQVGGPFLKRGMGSNSWSISASRTENRHTYLCNDMHMPLSQPSLWYGNHL
                     IAGDFNVTGVGLPGVPMVLVGHNARIAWGMTLAYTDCEDLFIEQFDTQNPHRYRFRDD
                     WLEAEIIPETIKVKGRPEAHVEKVVITHHGPVISDVVGYPEQRLAVRSMALQPCQAFY
                     GWYLLNQAADWDEFVEAVRLIDAPQLNINYADVDGNVGHWVSGKVPIRAKGDGNIPAP
                     GWTGDYEWIGEVPFEEMPHALNPAQGYIVSCNHRITPDDYPYFLGNVWMNGYRARRIV
                     DYFESRDVLKVADFQALHVDFTCLPGAEFVASLDDFKSDAPDVVLALKILRAWDGKLT
                     ADSVAGALYEVARYHIVRNLMEPALGEALTFHWMGQGFHPVLLPSNEFYGHDTLVMLR
                     LLSNPDSWWVVQAGGRHKVLSQGLAQAVRWLRAQLGADTQSWQWGRIHRAIFPHPLGL
                     QKPLDRVFNCGPFPIGGDTDTPCQTATHPNAPYDSNSWAPSFRQIVDMSDLSQSLVII
                     PSGQSGQLGSPHYDDMIQPWLKGEYIPMLWTREQVESHAEGKLDLKPSLPT"
     gene            12148..>14637
                     /locus_tag="AC16CB_04065"
     CDS             12148..>14637
                     /locus_tag="AC16CB_04065"
                     /inference="COORDINATES: protein motif:HMM:NF037660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3341677.1"
                     /translation="MTTSTLFSPSDLPTGTLTFLFTDVEGSTRLWELYPEEMRVAMAR
                     HDELIESYVVQSGGKVVRPRGEGDSRFAVFQYASDAAIAAIAIQRKFFTASWPFSEPL
                     RVRLALHTGEADLREGDYYGSAVNRCARLRGAAHGGQILISQTTQLLITEALPDGADL
                     LDLGEHKLKDLHRSERIYQIIIQGLPADFPPLKTPDSFLTNLPIPRNQLIGRNLEMET
                     ISDLLLLQDVALVTLTGTGGTGKSRLGLQIALDLRENFEDGVYLVALESIRDPKLVIP
                     TIAETLGVRESLGSRPITDILKEFLLNKQMLLLLDNFEQVVAAAPSVGDLLEACPRLK
                     ILVTSRTPLRLRAEKELPVSPLMVPTLEEYSNRSSLSQYAAVELFIQRAAAIKPDFAV
                     TNANAPAVAEICHRLDGLPLAIELAAARIKLLTPQGILTRLENRFELLRGGTRDLPER
                     QRTLRGTIDWSYNLLNELEKKLFQRLSIFIGGWTLEAAEMVCDIDGDLTQTLYDVLES
                     LIDNSLVIQIQDSEEGPRFGMLSTIHEYGRERLMESQEADTIHYQHAQYYLNFVQKVE
                     PLIRSAERMRWQQVMQQELGNVRGVLEWICRTGKCVEIGQHIVITLGIYWMISGYIAE
                     GQQWCNLITALSDESTPISIRAGLLGVAGEIAWMQGDHSSSKTNLDESLELLHRLDDK
                     TLEDKHILATVTMIRGLLAATSRDYETATTLYQRVIELCEDINDQWLEVITISWLGDI
                     ALYKNDSDRAMALYNQSIKLARQQGDPWSLMPSLMSSGQIALVKGDLANARSILNEAE
                     ALLRNTGDHWSLSWVLNDLGHIMLMEGKLDQA"
BASE COUNT         3759 a         3612 c         3700 g         3566 t
ORIGIN      
        1 tgctgagcct ccaagcacag gccaaaccaa ttgctgcgtc agacgagaac ggagaggcca
       61 aaggcccggt gtgcttcgaa gtcccatcaa attatgaaat aactgtcaat gggaagaagc
      121 tgttgggcag cgctcaagcg cgaaagcagg ggggaatact acaacacggg actttaccgc
      181 tttggggtga cctgacacgc atcacccagg ctctacatta tgaaagcgag agtcagagag
      241 aacgatccgc agcgcgccta caagagcgcg ctacaaccgt tgaggaaatt ctggggcatc
      301 gtcctacctg ggaggcagct actcgggcat tcatctcagc ttttaagcag gagctagcgc
      361 tggagctgga gccagctgaa ctctccgccg cagagcaagg ccgcgccatg gagttggtca
      421 gcgaaaagta tggaaatcct atctggactg agcggatcta gctgccagga attgttctct
      481 tatagccttt gaagatcgca agcgggtgag atcgccccaa ggtatcaatc ttgaggttca
      541 ggggttattc gaatgaagat cacttcaggg tagccaagat cgcgcaataa gcggtaaaga
      601 tagttttcag cattctggtt tgcttgctct aaaataccat cttccagggt tgctaattcg
      661 atctcatctt ccgcggctcg gcgagcttcg gtctctagat taatattccc cttggtcagc
      721 aggccggtat ctctattgta tatataagac ttatcgttat ccaaggtggc gacaaaaatc
      781 tccgcctcag ggagctgaac atacaaaaca ccgccacgca attgcatgtt ttcgggtgtg
      841 agtttttcca aatccacccc agcaatgaca atgccatgcg ccaccaggat caggcggtcg
      901 ccgaagagga aaccgaatgg accctggttg gtttcagcag tgatcacctt ctccacgctg
      961 tactggatcg tctccaggcg ggataacgag cgcaccccct ggataatggt cacgggatca
     1021 ggcaacacgg tgggggtagg gtttagaaat tgggccactt gagtggcgac attgctggtc
     1081 aattcgttta ccggctggat agcctgcttg gcctggttag cactctgttg cacaacagat
     1141 accacgccaa atgccgctag ccccaggatc aacaacacgc caatgaccag aacccaacca
     1201 cgaagattat tgtcatttgc cataaaaata attatactgt aagtacctac gcagttgaag
     1261 aaaagaccct atgcgtatct gttgtcgata taggctatga ccctaggtat catcaccctt
     1321 cacttacaaa taccaggatg cacctcgctg aaagagaaac gtcggcggtt gaaacccctt
     1381 ctggcgcgct tgcatcgcca attcaacatc tcagtagccg agatggatca acacgacgcc
     1441 tggcaagagt cactcatcgc ctgcgcagtg gtgagcaacg atattggatt caccacgcgc
     1501 agcctgcaag ccatacccga ctgggttgag gtgcactggc cagacgtggt tgtgctcgac
     1561 aaccagattg aagtattcta gagactgctg aaaataacaa ttgggggatt ttatctaatg
     1621 tttattaaag tagctcgatt tttttcgttg tatatatata tatcaagcca taatttagaa
     1681 accgagctaa ggtgcgggtg ttttaagaat taagaaactc gttaattact ctttttacga
     1741 tctttcgata acgctatttc tgcgcgttga gcatattgag aagcaggcca cgtatccaaa
     1801 acagcagtaa actccagcct agcctggggt tccatatcct tacgccgata agctatgcca
     1861 aggttgtagt gggtagcagc ttcatattta actccaaggt aaccctgatt agcatttttt
     1921 aagacatctg taaaaacagc aatagcctcg tcgagggcat cattttgtaa aagcgctgtt
     1981 ccttggttca cttgtatcac caaatgaccg gtttgatcgg gccataacct ttgagcaaga
     2041 ttatagaaga gatttgccct ctcaaattga gagcgctgtg caaaaaagtt agcgatgtca
     2101 accaatagcc tgactcgcat ggttatcagg taaagtacga tcagaaacat gattggatgg
     2161 atctgtattc cggttaatgc agtcaaacca cccaccataa aggtgatcac caatgcttca
     2221 atagcaaatc tacttgataa cccttcccga cgaaggagtg ataatcctcc aaagaggaca
     2281 atataggcaa gacctacaag gatgatcagc gctggcaaac tcacataaac ctccgaaaag
     2341 atagctgaat accagttagt atacaacaaa tgagaggatt atggtgttct ttgtaacacc
     2401 tcacatgtaa caatctacat ttgacttatg agaacaaatt cctctacaat cgagtatgcg
     2461 cccaaaacat ggagaagatt tgttcggatg atcttcaaat atatccgtag agggggatca
     2521 gaggattgtt cttgtcaaag gaggtgccat cgataattct gctctaataa taacaattta
     2581 gatcaaatat tttgtagaaa tgaggagaat ttgcgatgaa tgcagtctta acccttgttt
     2641 taggactggt gggcatcggc attggctatg gcttttatgc cagaaccatc aatgccggcg
     2701 tcgtcaaacc ggatgacaag aaagctacgc cagctaggat gtttatggac ggcgttgact
     2761 tcacccccgc taaccgaaat gtattgttcg gataccaatt caaatccatt gcagcgttag
     2821 ggccgattgt agggccgatt gtagccatcc aatggggctg gttacctgct ctattatggg
     2881 tgatcttcgg cacattcttc atcggctggg tgcaagacta ttcggcaatc atcatgggcg
     2941 tgcgagaaga gggtcagagc tttggcgcgc tgagctatcg ctttgtttcc cccagagctc
     3001 gaacgatttt gttgatgttc atttacttct acttgttgct catcatgggc gcatttggaa
     3061 aaatcgtcgg ctatgacctg atgaccaatc cggcagttcc attgggtgtg ctattggtga
     3121 ttggtgtagg tttgctggca ggacagatga cgtacaagtg gaagatggat atcattctaa
     3181 ccagtgtagt cactgtcgta ttagccttct taggtatttg gctcggcaca ttacctggtg
     3241 tgaaatcgct cttcctggca atcaatggag gcgaaaaatc acccgtggtc tttggctcag
     3301 tcacgcaagc ccagttcatt tggagcctgg tagttctggt aatctgctac tttggtgccg
     3361 tactgccaat ctggcgctgg gcgcaaccga tcaactacgt ggctttctgg atcgtcttcc
     3421 tgggtattgt tgctggcacc atcggcttgc tggtttggca tccatccttc ccatccgatt
     3481 tcccagcatt tacgaactgg gcaggcaatc tatgccaaac agccgactgc acaggagtag
     3541 agaaagcacc acttgcccta ggaccacttt ggccgattct attcgtgacc atcgcctgtg
     3601 gcgcagtatc tggatggcac agtttggtgt cttcatcggg aacagcacgc cagcttgaaa
     3661 aagagaccga tgccttgtat gtaggcggtg gcgccatgtt cctcgaaatg ttcctggcaa
     3721 cactgtccat tctcacagcg gtggtaggcg taggcgtcac acgaggcttc gtaggcacag
     3781 ccgataaacc aggatatgtg cttctggtag cggctggcaa gaacgctggt atatttgcta
     3841 ccggcctaag tgagatgatg gcgcgggttg gtgtcccgac cagcttcggt ttgccctatg
     3901 gttcggtctt cctgacgctg atggctctca cgatcatgta cctggtggta cgcttcatgc
     3961 gtgtggcctc tgctgaattt ttgggtgatt ccgtatccat attcaggaat gtaacctttg
     4021 gctcaatcgt cgctctggct ctctccgccc tgctgatctg gaccggcttc tgggcacgca
     4081 tttgggtact ttttggaggt gctaaccaat taatggcggc gctggctttg ttactggtta
     4141 cgctgtggct tatgtccaat ggcaagaaat atatgtggac gcttgtcccc ttcttgttca
     4201 tgttcgtaac cacgatcgcc gcattgatca tcacagctta caatgtattt gctcagatcg
     4261 ctagaacgcc cgatatgcca attgacggct tgattggtaa cgtcatcgcg ggtgtgattg
     4321 ccatcttcct ggtgatcgca gcgctaatcc tggcatggga tgccctgaaa gcgttccagc
     4381 gaattcgggc tacggcacct gccaaagcct gataatgcca tacaagagga gttgcgcgct
     4441 gaaagctggt acacaactcc tcttttccac atttcaatga agcccgtaca ggttgaggtc
     4501 atttccccat tacccgagag ttgggggatg tgtttgtcat gtgaaatgtt attggcacgc
     4561 gccaatctag ataaagcgcc atacgagcga ggattagatg aatatccgcc cgaatggcaa
     4621 gaagaattcc agcgcttggc ctcttttgtc atggacctgg ctgtacaata tggtgaccaa
     4681 atacaaattc gcatttggga tccccgctct cttcaagggc ttgccaaatc gctacgctat
     4741 ggcgtgcggc gttatccaac ctttgtggta gatcgtcgtg tcaagataac aggctgggac
     4801 gtatctggcc tggaacaagc cttacttgca gcatttgccg cataatattc atgagctcaa
     4861 aaaaatactt caggtcctta ctatttcaaa acgtactata atcaaatcac tgtgaataac
     4921 caacccagtc tcaccaggtg gaaatctttg atcagacaga tcgcgaggac gactggtgat
     4981 attttctatg gggctaccat ttatgagatg gtgcgtgact tgaaaaagga gcgttcccat
     5041 cttgagcatt tgttcatact gatcgtcttt ggggacctgt tgggcgtacc tgtcttgcct
     5101 ccctattaca ccttacgtct attgccttat gttatgccag atataactgg ctggaaacat
     5161 agtcttctac gtgagcgaga tttgaccgat atgtgtgacg tggaaattac ataatgtgag
     5221 gctctacaat accgggaagt ataattgtaa gatatagata ccatcatcat gaggaaaaaa
     5281 catggctgac gagaataaaa aaccaagctt tttggatgga tttaaaagcg tcctatatgg
     5341 catggctgga cacgacatgg cgcgttatgc acttaagaca cgcgggagca tggaacacct
     5401 ctttattctg atcacgatgg gtgacctctt gggtattccc attcttccgc cctattatag
     5461 cctgcgcctt ctcccttacg tggtccccca aatcagcacc tggaagcgcc gcatgttgcg
     5521 cgaaaaagat ctgacagacg ctctggcgta gctgtagtgt ggatgtaacc aacaaatcct
     5581 aaccaggtgg atacggggag gaaaggcatg acgctggtta gcgccttcgg gtcgccagcc
     5641 tcattaggat gagaaaaaga atagacggag aatacggaga acgttatgtc tcttcgagaa
     5701 gtgtttgaaa aaaatcctga aagacggtac atcatgtttg gggggaaggg tggattggga
     5761 aaaactactt ttagcgccgc aacagccttc tggctggcaa aacaaggtta taaagtactc
     5821 gttttttcag tcgatcctca agccagtcta tccgacatct tccagcgcga catttttggt
     5881 aaggggccgg tagaaatcat gcccaatctc tatgcccagg aaattgacgc agatcggcgc
     5941 atccacgaat atcaggagga gatccgccag aaaatccgcg agatgtatgg gttagagcag
     6001 ataccagaag agattgagaa ttatatccag gctgccgctg cagaaccagc tatggaagag
     6061 agtgctatct tcgatgaggt agtagacatc gtcgtcggtg gtcagtatga cttttacatc
     6121 tacgatttag ttcccttagg gcatgcgtta tattatctaa gtatggcctc cgtgtatgat
     6181 gagtggatcg ataaaatcac taaattacgc gaggagatgg cagagtacga tcaggtagcg
     6241 gctgtgatga agcgcgaaaa agaatccgaa gaggacatga tcctcaaaga attgcaatac
     6301 atcaagtaca ggattaatac ctcctcaagt atattgaccg acaaacaaaa gacggctttc
     6361 tttttcgtcg tgacacccga agaaatgata attctggata cacagaaggc agctgggcta
     6421 tttgcgaaat ttgatgtacc catcgcaggc tatatcgtca atcgtgtcat ccccccagaa
     6481 cttacgaaac aaaagattcc cgattatcta cgaaaccggt tgacaatgca aaaggactac
     6541 cttactgaaa ttgatcaggt gttcggtggc caggttttag cccgcgtacc cgaatttgag
     6601 cgcgatatta cggggctgac aatgattgag caaatggccg atgccatgtt cggaggtggg
     6661 gcatgataga aaaatccatg gtacagtttt tatctgacaa ccctcgccta aaatatatat
     6721 tctttggggg aaaaggaggc gtcggtaaga ctgttctcgc tggcactgct gcactctggg
     6781 cagcccaaca aggcaagaag acattacttg catcaacaaa ccctgttcat tctctgtcca
     6841 gcctgctaga tcaaaacgtg ttcggcaaac cgactcctgt ttccggtgct ccaaatttgc
     6901 tggcttatga gatcgatact cgtgaaacga tcgaaaaatc caaaaaggaa atccgcgaga
     6961 agatcgagtg gtttctcaaa tttgccgata tcaaaacgaa ggcagatgaa tttgtcgaat
     7021 cggctaccat gaatcctgcc tttgaagaat cggcgatgtt cgagaacatg atcgatttga
     7081 tgttcgagga caagtatcaa gtatatgtgt ttgacactgc accgacggca aacgcccggc
     7141 gacttttggg gatgtccagc gtatattcca tgtgggtcaa caagatggtc caaagccgcg
     7201 aagaagccat gtccttgaaa gacctgctct cgtatagcaa aaagaaaaaa gagaaagacc
     7261 cccttatgga ttacttattg aatttacgtg agcggatggg acgcgcaaag atgctcctaa
     7321 ccgacgctga actcaccgcc tttttcttta tcacattgcc cgaagccctg cctattgctg
     7381 tgattactcg ctttatcaac tggtttcatg actttggtat accggtgggc ggagtaatcg
     7441 tcaacatgca aatcgacaag accagcttgg gaccagatgc gccggatttt gtaaagaacc
     7501 gtgttgcgat gcaagatgag catatgctga ccatctggaa agaattcgat ggccaggtgc
     7561 gtgcgatcct gcccctactt gaaaccgagg tccgcggagt tgatatgctg aaacgcatgg
     7621 catcgctgct atacgctacg tgaattcctg atatttgtat cgaataaata cgagagtaga
     7681 tagcgatatt gggtgtgtgc agtgaaaccg cacacaccca tccaaaaatt atcgcaatga
     7741 aatggcaaaa tcccgaattt cccccaggaa cttctcagca ggctcgactt gcaaggcgga
     7801 aagatttgcc ctcataagca aatggggatc ctttctctga atggagacac gcgttatccc
     7861 tctattatat tttttcaaca attcctgcag gtgatccaaa gagtcaaggt ccttacggcc
     7921 gcggtacgcc agcacaaacc acggtgggac agacatcgat tcatagggtc gtttttcttc
     7981 accatccacc agatttttaa actgctgatc tcctttatac gcagcctcca actcctgaga
     8041 tggcacacgc aataaatttg acttcaagac aatctcatca cgcgtaccca acaggcgatt
     8101 gtaaatccag agcggcaggt tttcgcgtgc ttccaataga aatgctacct cgaaataccg
     8161 gaaaggagct tctctacaat caacgtgaaa ttgcgctccc caggcagcag tgcctttcaa
     8221 gcgagatacc ttcacgttac catacagact aaggccggta tacaaccatg ctgcaaccgc
     8281 tttagacttt ctgcggttat aataaaatcc ggtcgcgtac catagcccaa taaaaatgca
     8341 aaggccgatg acaatccatt gaccaacatt catgcgaacc tcccgcgtga ttatttctcc
     8401 agtttagccc aagaggggct caatgtaaag attctataaa tctaatgagg agagaacttt
     8461 atggatctgg gattaacgaa taaacgcgcc ctcgtgacag gatcaagtcg cggtctgggc
     8521 tatgaaaccg cgcgcctcct ggcaatggat ggttgccggg tggtcatcaa tagccgcagt
     8581 tcggagaacc tgttcacggc ggcaaagaag cttgaagaag agaccggggg tcaggtcttt
     8641 ccactggtcg gtgatgtaac cgatccagcc ataccggaac aacttgtgcg gaagtctgcg
     8701 gaggcgctcg gtggcttgga cttactggtc accaattctg gcggacctcc atccggcaaa
     8761 ttcgaaattt ttgatgacgc aacgtggcag aaagccgtag aattgaactt tctcagccat
     8821 gtgcgcctga tccgcgccgc tctaccctac ctgcggcaga gtaaagcagc cagcgtgctt
     8881 gccatcactt cctattcggt caaacaacct atccccaacc tggtgctctc caatagcgtg
     8941 cgggctgcca cagtcggcct tattaagacc ctcgcccttg agttaggggg ggatgggatc
     9001 cgcttcaact ccatcctgcc tgcctggacc gagacagaac gtgttattga gctaatggct
     9061 tttcgcgccc agacgaacgg taccacggtc gaggaagaga tcgccaaaca agccaaagat
     9121 agcccaatag ggcgcatggg ccaccccgag gaattcgcta acgccgccgt tttccttctc
     9181 tcgccggcag cttcctatat taccggcgtg atgttgactg tggatggggg aatgttcaaa
     9241 gggacactat agccagtcat acaagcaatc ttatctggct ggtctgagca tatagaccag
     9301 ccagattctt tcagcctaag ctgcttatct agtagataga atacgaggtc accatatgtc
     9361 tgccatatta aatatcgctg gatctatcgc tcgccaaggg ttaacctggc tggggcgcag
     9421 gcgcttgcca cagatcacag gtaatattga tatccccggg ttgcatgcgc cggcagagat
     9481 catccgcgat cgttggggca taccccacct atacgccaat gacagacacg acctgttctt
     9541 cgcccaaggc tttgtccacg cacaggatcg cctgtttcag atggaattga acagacgcac
     9601 gtcaagcgga tgcctgagtg aaatttttgg tccgctcgcg ctggatactg acagggcatc
     9661 tcgcactttc ggcttcgctc gcctgggacg tatagattgg gagaacgcaa atgatgagct
     9721 gcgcagcgtc atcctggcct acaccgaagg ggtgaacgcc tacctgaacc acccggatag
     9781 caagctaccc gtcgagttta ccttgctcgg ctaccatccg gaactatgga agcccgagga
     9841 ttcgtccgca ctggcgcggt tcatgatgtg gcagctttcg cacgcctggt acggagagat
     9901 tgtgcgagca caactaatcc aggctgtagg agcggagcgc gcagccgagc ttgaaataca
     9961 cattccttca caaaatccca tcacactacc acgtggcatt gaatttaacc gcctgcagcc
    10021 cggagagggg ttgcagcaag taggggggcc attcctcaaa cgcggcatgg gtagcaatag
    10081 ctggtctata tcggcatcgc ggactgaaaa tcgccacacc tacctgtgca acgatatgca
    10141 catgccgcta tcacaacctt ccttgtggta tggaaatcat ttaatcgcgg gagatttcaa
    10201 tgtcactggc gtaggcttgc caggcgtccc tatggtactc gttggacata acgctcgcat
    10261 cgcctggggg atgacgttgg cttataccga ttgtgaggac ctctttatcg agcaattcga
    10321 cacccagaac ccccatcgct atcgattccg tgacgattgg ctcgaagccg agatcatccc
    10381 tgagaccatc aaggtcaaag gtcgcccaga agcgcacgtg gagaaggtag taataaccca
    10441 tcacggcccg gttatctctg atgtagtcgg ttatcctgag caacgcctgg cggtcagatc
    10501 gatggctctg caaccctgtc aggcgtttta tggttggtat ttattgaacc aggcagcgga
    10561 ttgggacgag tttgtagagg ctgtgcgcct gatcgatgcg ccccagctaa atataaatta
    10621 tgcagatgtc gatggcaacg ttggacactg ggtgagcggc aaggtcccta ttcgagccaa
    10681 aggtgatgga aacatcccag cccctggctg gacgggcgat tacgaatgga tcggcgaggt
    10741 accttttgag gaaatgcccc atgccctcaa ccccgctcaa ggctatatag tttcttgcaa
    10801 ccaccgcata acaccggatg attaccctta cttcctgggc aatgtgtgga tgaacggata
    10861 ccgcgcccgt cgcattgtag attactttga gagcagggat gtgctcaagg tggcggattt
    10921 ccaggctttg cacgtagact ttacctgcct gcctggggct gaattcgtcg ccagcctgga
    10981 cgattttaaa agcgacgctc ccgatgtggt attggcatta aagatcctgc gcgcctggga
    11041 cggcaaactg actgccgatt cagtggcagg tgcactttac gaggtagctc gctaccacat
    11101 cgttcgcaac ttgatggagc cagccttggg ggaggcattg accttccact ggatgggaca
    11161 gggtttccac ccggtgctct tgccttctaa cgaattttac ggtcacgata cgctggtcat
    11221 gctgcgactg ttatccaacc cagattcgtg gtgggtagtg caagctggtg ggcgccataa
    11281 ggtcttgagc cagggtttag ctcaggcggt ccgctggctt cgcgctcagc tgggagcgga
    11341 cacgcaatcc tggcaatggg gcaggattca ccgggcgatt ttcccccacc cattgggtct
    11401 gcaaaaaccg cttgaccggg tcttcaactg cggtcctttt cccatcggcg gggacacgga
    11461 taccccctgc cagaccgcca cccatcccaa cgctccttac gacagcaact cctgggcgcc
    11521 atccttccgc cagatcgtcg atatgagcga cctttctcaa tcgctggtca tcatcccgtc
    11581 agggcagtct gggcaattgg gtagcccgca ttatgatgat atgatccaac cctggctgaa
    11641 gggtgaatat atccccatgc tgtggacgcg ggaacaggtg gaaagccacg ccgaaggaaa
    11701 actagatctc aagccatccc ttccgactta gaactggcag ggattggtcc acgccaaaca
    11761 cattcgcctc gaggcagaaa ggcataatca agatcgcctg gcatgtatga actatccatc
    11821 gccgggggaa gcttaactga acatccccaa ggtgcgaatt aggggctggg ctcataaccc
    11881 atagctcttg attcgaatcc aagccccgct actctgaaaa agacaccaca aagttccttt
    11941 gattcgttat aatagtgcga aatctggatt ctgagtaagg ggatagcaat cgttgcacat
    12001 gaggtcccca tggcgatagt tcgatcactg ggaactgcag ggttttgacc atcgacaaga
    12061 agaaatttct gcgccatatc catgaatatg cctcgaaaat attctaaggg agagtggtgg
    12121 ttgatccgaa atggttaggc caaattgatg accacatcga ctctattctc accttccgat
    12181 ttgccgaccg ggacgctgac atttctattt accgatgtag aaggcagcac gcgcctatgg
    12241 gagttatacc ccgaggagat gcgggtggcc atggcgcgcc atgatgaatt gattgagtcg
    12301 tatgtagtcc agtctggggg caaggtggtc cgaccccgcg gagaaggcga cagccgcttt
    12361 gccgtctttc agtacgcttc agacgccgca attgctgcca ttgctattca gcgtaaattt
    12421 ttcacagcat catggccttt ctctgaacct ttgcgtgtgc gcctggctct gcatacgggt
    12481 gaagccgacc tacgtgaggg cgattattac ggaagcgcgg taaaccgctg tgccaggttg
    12541 cggggcgccg ctcacggcgg acaaatcctt atatctcaga ccacacaatt attgataaca
    12601 gaggcattgc ctgatggtgc agacctgctt gacctgggtg aacataaact caaagatctc
    12661 caccgttctg aacgcatata tcagatcatc atccagggat tacccgctga tttcccacca
    12721 ctaaaaacac cagatagctt tctcacgaac ctaccaatac cgcgtaacca gctgatcggg
    12781 cgcaatttag agatggaaac gatatccgat ctgctcttgc tccaggatgt cgccctggtc
    12841 acgttgaccg gaacaggagg gacaggtaaa tcccgcctgg gcttacaaat cgcgctggat
    12901 ttgcgtgaga actttgaaga tggggtttac ctggtagcgc tggaatcgat ccgcgacccg
    12961 aaactggtga ttccaaccat tgccgagacc ctgggtgttc gcgaatccct tggcagccgg
    13021 ccaataaccg acattctcaa agaattttta cttaacaaac agatgctctt gctgctggat
    13081 aattttgagc aagtagtggc agctgcaccc tctgtagggg acctactcga agcctgccca
    13141 cggttgaaga ttttagtaac cagccggaca ccattacgcc tacgggcaga gaaggaactt
    13201 ccagtttcac cgctgatggt tccaaccctg gaggaatatt ccaacaggag cagtctctca
    13261 caatatgctg cagtcgagct atttattcaa cgcgcggcag cgataaagcc ggatttcgca
    13321 gtaaccaatg ccaatgcacc cgccgtggct gaaatctgcc accgcctgga tggattgccg
    13381 ttagcaatcg agcttgcagc agcgcggatc aagctgctca ccccccaagg gatactcacc
    13441 cgtctggaaa accgctttga gctcctacgc ggcgggacgc gtgatcttcc ggagcgacaa
    13501 cgcaccttgc gcggcactat cgactggagc tataaccttc tcaatgaatt ggagaagaag
    13561 cttttccagc ggctatcaat ttttatcggc ggatggacgc ttgaagcagc agaaatggtc
    13621 tgcgacatcg atggagactt aacccagact ctgtatgatg tgctcgagtc gttgatcgac
    13681 aatagcttag tgattcagat tcaggatagc gaggaaggcc cgcgctttgg gatgctcagc
    13741 acaatccatg aatacgggcg cgagcgcctg atggagagtc aggaggctga taccatacat
    13801 taccagcatg cccagtatta tctcaatttt gtccaaaagg ttgaacccct catccggtcg
    13861 gctgagcgta tgcggtggca acaagttatg cagcaggaac tcggaaacgt acgcggggtt
    13921 ttggaatgga tctgcagaac tgggaaatgc gtcgagatcg gacaacacat tgtcatcacg
    13981 ctggggatat attggatgat aagtggctat atagccgaag gacaacaatg gtgtaatcta
    14041 atcacagcgc tgagtgatga atcgacacca atatcgatcc gcgccggctt gttgggagtt
    14101 gcgggtgaga ttgcctggat gcaaggcgac cattcctcat cgaagactaa tctggatgag
    14161 agcctggaac tgctacatag gctggatgac aaaacgttgg aagacaaaca catattagcc
    14221 accgtcacga tgatccgagg cttattggcg gcaacctctc gcgattacga aacagcaact
    14281 acgttgtatc aaagggtcat tgaactttgc gaagatatta atgaccaatg gctagaagtt
    14341 ataacaatat cctggttagg tgatatcgca ttgtataaga atgatagcga tcgcgccatg
    14401 gctctctaca atcaaagtat caaactggca cgacaacaag gcgacccctg gagtctgatg
    14461 ccctccctga tgtcatccgg gcaaattgcc ctggtgaaag gggatcttgc caatgcccgc
    14521 tcaatcctca atgaggccga agctctccta cgcaatacag gtgatcattg gagtctttca
    14581 tgggttttga atgacctagg ccatatcatg cttatggaag gcaagcttga ccaagca
//
LOCUS       DBNGSX010000121         4438 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_701629, whole genome shotgun sequence.
ACCESSION   DBNGSX010000121 DBNGSX010000000
VERSION     DBNGSX010000121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_701629"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..836)
                     /locus_tag="AC16CB_04070"
     CDS             complement(252..836)
                     /locus_tag="AC16CB_04070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016107.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR3341678.1"
                     /translation="MIILLAVFVYSLFHSILASLWVKARARAWFGPETERWYRLAFNA
                     IAVVSLVPVLTLPALLPDRNVYTIPFPWAYINLAIQGLAGLALIVGLWQTGLWSFLGI
                     QQFLTPPKSDPPKLVVGGLYRWVRHPLYTAGLALIWFIPVMTTNLLALILGLSIYLVV
                     GALFEERRLLVEHGEAYAEYQRRTPMLIPTRFPR"
     gene            1022..1762
                     /gene="lexA"
                     /locus_tag="AC16CB_04075"
     CDS             1022..1762
                     /gene="lexA"
                     /locus_tag="AC16CB_04075"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIR3341679.1"
                     /translation="MMARRRTKGLSERQKRILEVLERFQNQNGYPPSIREICEKTNIS
                     STSVVNYYLNQLEEAGYIERDGRVSRGIRLLRPLTEITGSAAAALKQTAQSVRETVEE
                     LLHIPVVGRIVAGEPVPVPQSDFSYFDADSMVDVARSLLPAREKGSSNLFALEVQGNS
                     MIDAMVNDGDIVIMKQAREARNGEMVAVWLNDRDETTLKYFYLENGTVRLQPANPTMD
                     PIFIDDPSLVEIQGKVVMVIRKVGGLPS"
     gene            complement(1834..3114)
                     /locus_tag="AC16CB_04080"
     CDS             complement(1834..3114)
                     /locus_tag="AC16CB_04080"
                     /inference="COORDINATES: protein motif:HMM:NF013125.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005882 - intermediate filament [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HIR3341680.1"
                     /translation="MSAPIHAQSVQTQDLNFMPIILKNWPPPTPTPSPGSVLISEVMY
                     SPKNLDTESEWIELYNPGDFAYDLSGHKLGDEEIQGENEGMYLLPDGTILSPHATIMV
                     AYRAEEFYSLYGFYPDFELFNTSISVPELPRYTDWASGILGLDNSGDEVIILDGSDQM
                     LDIVVWGNGYPLFYPTVDNVALGHSIERYPPFQDSNTAQDWRDQPDPSPAAVDNTPPT
                     PTPSATPTSINTPTPTPTATPTQTQTPTPTPTMIPGLVINEVHADPDADFGDANGDLV
                     VDTYDDEFVEIVNATGGAIDLNGWRLNDRVGERHVFPPGSLLPNGCSVVVFGGGNPSG
                     GFGGSLVQTASTGGLSLNNEGDRLTLYNASSTAVAAIGYGAEANDDQSLTRYPDITGP
                     EPMVKHATIPEAGGKLFSPGKRLDGAVFSGCPAN"
     gene            complement(3277..>4438)
                     /locus_tag="AC16CB_04085"
     CDS             complement(3277..>4438)
                     /locus_tag="AC16CB_04085"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR3341681.1"
                     /translation="AIVSSEPRVNVGATFSGVITGVMDYSLGNFKLFNTEPFPSITAT
                     GVTSETVSEASPDQLSVVTLNTKNLDPEESPSKYARLARQIVGYLLSPDILVLEEIQD
                     SNGSLNDAVVTATLTYSMLVTAIQEAGGPLYQVRQIDPLDDQDGGQLGGNIRVGFVFR
                     TDRGLSFVDRPGGDAITPVTATLGTNESQISISPGRVDPLNQAFNESRKPLAGEFIFK
                     GHKLILIACHLNSRSDDSLLFGRYQPPRQYSQAQRIQQAGAVNAFVHHILTLDPLANV
                     IVLGDFNDFPFSTILAALQGEILTNVLDQIPEAERYTYIFEGNSQALDHILVSDNLLQ
                     TSLDEVDIVHLNAEFAEATRPTDHDPVMVRFSFEEIRHAIYLPILSRATPPE"
BASE COUNT         1073 a         1132 c         1227 g         1006 t
ORIGIN      
        1 cttttgaagg taggctagta tgctggttgg atcgtagatg actccgctct cattgatctc
       61 ctgccagggt tgaagcgtgc tatactccat gtggtcctcc tcagtttttt ggctgtcaga
      121 acatccattt tactgaagag gactagtttt attcaattgt caaggaactt tgagaacgcc
      181 atcccgccct ccgaacatac aaaaaaggaa ggagtctcac tttcaaaggc gtgcaatata
      241 gtgagcattt tttatctagg gaaacgagtt gggatcagca tcggtgttcg cctttgatac
      301 tcagcatacg cctcaccgtg ctcaaccagc aacctgcgct cctcgaacaa cgcccccacc
      361 accaggtaaa tgcttagacc caggatcagc gccaggagat tagtggtcat cacggggatg
      421 aaccagatca aggccaatcc agccgtatag agcgggtggc gcacccagcg atacagccca
      481 cccaccacca gctttggcgg atcagattta ggaggggtga gaaattgctg aattcccaag
      541 aaagaccata ggccagtctg ccacaagcca acgatcaacg ccagccctgc cagcccttgg
      601 atagccaagt tgatgtatgc ccatgggaag gggatggtgt aaacgttccg gtcgggcaac
      661 agagcgggca aggttaacac cggcaccaaa ctgaccactg cgatggcatt aaatgccagc
      721 cggtaccaac gctcggtttc cggtccaaac caagcacgag cgcgcgcttt aacccacaaa
      781 gaggccagaa tcgagtggaa taagctgtat acaaaaacag ccaggaggat aatcacaaat
      841 gatctcatcg gataggttgg caatagttta tcgcaattcg tcaaatatgc aaactacccc
      901 gttaaattcc taaaaaacag gtcttgacac agaacgaatg ttctggtaat atatttttag
      961 cacaaatgaa cgaattaatg gatgtccttt aatttgccaa gattggacag gagaaaagac
     1021 tatgatggca aggcgacgta ccaaaggatt gagtgagcga caaaagcgta tcctggaggt
     1081 gctggaacgt tttcagaacc agaacggata ccctccgtcg attcgcgaga tctgtgaaaa
     1141 gaccaatatc tcttccacat cggtggtcaa ctattacctg aaccagctag aagaggctgg
     1201 ttatattgag cgcgatggcc gggtctcgcg aggcattcgc ctgttacgac cgctgacgga
     1261 gatcaccggt tccgcagctg ccgccctgaa gcagaccgcc caatcggtgc gcgagacagt
     1321 cgaagagcta ctccatattc cagttgttgg gcgcattgta gccggcgaac cggttccggt
     1381 gccacagtcg gatttctcct atttcgatgc cgatagtatg gtggacgtcg ctcgcagcct
     1441 gctacccgcc cgtgaaaaag gtagcagcaa tctctttgcc ctggaagtac agggcaactc
     1501 catgatcgac gccatggtca atgatggcga catcgtcatc atgaagcaag cccgtgaagc
     1561 ccgcaatggg gagatggtgg cggtatggct gaatgaccgc gatgagacca ccctgaaata
     1621 cttctatctc gagaacggta cggtgcgcct gcaacctgca aatcccacca tggatcctat
     1681 atttattgat gacccctcct tggtggagat acagggcaaa gtagtaatgg tcatccgcaa
     1741 ggtgggtgga ttgccttcgt aaaatcctct cgcctcctct ggaggaagcg aaggcagtgg
     1801 taaaggaacg ggctgcaacc agcccgttcc tgcttaattc gcgggacagc cggagaatac
     1861 agccccatct aggcgtttgc cgggcgagaa gagcttccca cctgcctcag gtatggtggc
     1921 atgtttcacc atgggttccg ggccggtgat atccgggtag cgcgtcaggg attgatcatc
     1981 attggcctcc gcgccatacc caattgctgc caccgctgta gacgaggcgt tatataaggt
     2041 caaccggtct ccttcgttgt ttaaagagag gccgccagtc gaggctgttt gcaccaggct
     2101 gccgccaaaa cccccgctgg gattcccacc gccgaagacc accacagagc aaccgttggg
     2161 aagcaacgaa cctgggggaa agacatgccg ctcgcccaca cgatcattta gacgccaccc
     2221 gttcaggtcg atggccccgc cggtggcatt gacgatttcg acgaactcgt catcgtaggt
     2281 atcgaccact aaatcgccgt tggcgtctcc aaagtcagcg tccgggtcgg catggacctc
     2341 attgatcact aggcctggga tcatggtagg tgtgggggtg ggcgtctgtg tctgggttgg
     2401 cgtcgcagtg ggagtggggg tcggcgtgtt gatcgaggta ggcgtggcag atggcgtggg
     2461 ggttggcggc gtgttgtcca ccgccgcggg cgacggatct ggctgatcgc gccagtcctg
     2521 ggcagtgttg ctatcctgga agggcgggta gcgctcgatg ctgtgaccta gagccacatt
     2581 gtcgaccgtc ggatagaaga gcgggtatcc gtttccccac accacgatat ccagcatttg
     2641 atcgctgcca tcaaggatga tcacctcatc ccctgagtta tcaagtccca atatcccact
     2701 tgcccaatca gtataacggg gcaattccgg cacggaaata ctggtattga acagctcgaa
     2761 gtcggggtag aacccataaa ggctgtaaaa ttcctcagct cggtaagcga ccataatcgt
     2821 agcatgcggg ctgagaatcg tcccgtctgg aagcaggtac atgccctcat tttccccctg
     2881 gatctcctcg tcacccaatt tgtgaccact aaggtcatag gcaaaatctc ccgggttata
     2941 tagctcaatc cactcgctct cagtgtccaa attcttgggg ctgtacatga cctcgctgat
     3001 caggacactg cccggggaag gtgtaggcgt ggggggaggc cagtttttta atattatcgg
     3061 catgaaattt agatcttgtg tttgaacgga ctgggcgtgg atcggtgcgg acatcctcac
     3121 caacgcgaag gccagataaa ccaacaagac gaatattaga aatttgtctg tttggcgacc
     3181 tggtccctgc agcatattga tattgtatgg tcaatatttt attattgact tatcactttt
     3241 aataactgga attgtcatcc ctgtttgaga aactggtcac tctggaggcg tcgcccggga
     3301 aagaatgggt agataaatag catggcgaat ttcctcaaag gaaaaacgca ccataacagg
     3361 atcgtggtct gtggggcggg tagcctcggc gaactcagca ttcaggtgaa cgatatccac
     3421 ttcatctaaa gatgtttgca gcaggttgtc gctcaccagg atgtggtcca acgcctggga
     3481 gtttccttca aagatataag tgtacctttc agcctctggt atttggtcca atacgttggt
     3541 cagaatttca ccttgcaagg cggctaggat ggttgagaaa ggaaagtcgt taaaatcccc
     3601 caatacgatc acatttgcca gcgggtccag cgtaaggatg tgatgaacaa aggcattgac
     3661 cgcgccagcc tgctgtatgc gctgcgcctg ggagtattgc cggggtggtt ggtagcgccc
     3721 aaataaaagg ctatcgtcgc ttctcgagtt gaggtggcaa gcgatgagga ttagcttgtg
     3781 acccttgaag ataaactcac ctgccagggg cttgcggctt tcattgaagg cctggttcag
     3841 tgggtcaaca cgtcccgggc tgatagagat ttgggattcg ttggtaccca gcgtggctgt
     3901 gaccggagtg atggcatccc ctccagggcg gtcaacaaag gataaacccc ggtcggtgcg
     3961 gaagacaaat ccaacgcgaa tattgccgcc aagctggcct ccatcctggt catccagggg
     4021 atcaatctgc cgaacctggt aaagcggtcc gcccgcctct tggatggcag tcactagcat
     4081 cgagtaggtg agcgtggcgg tcaccacggc atcattcaag ctgccattgc tgtcctgtat
     4141 ctcctccagg accaggatgt ctggagaaag gagatatccc acaatctgcc tggctagtct
     4201 ggcgtatttg ctagggctct cctcggggtc gaggttcttg gtattaaggg taacgacgct
     4261 gagctggtcg ggtgaggctt cggataccgt ttccgaggtt acccccgtgg cggtgattga
     4321 aggaaagggt tcggtattga acagcttgaa attaccaagg ctgtagtcca tgacgccagt
     4381 gatcacaccg ctgaaggtgg ctcctacgtt cacccgcggc tcggaagata caatcgca
//
LOCUS       DBNGSX010000122         6090 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1048516, whole genome shotgun sequence.
ACCESSION   DBNGSX010000122 DBNGSX010000000
VERSION     DBNGSX010000122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6090)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1048516"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(329..1105)
                     /locus_tag="AC16CB_04090"
     CDS             complement(329..1105)
                     /locus_tag="AC16CB_04090"
                     /inference="COORDINATES: protein motif:HMM:NF013752.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR3341682.1"
                     /translation="MEIYLNAPKPGQPHLDGHHPGYDSKIAWTWVPHVRGHSSARKQL
                     ALLAYVRRLIPAKAAVFLVGDCEFGAVDVLKQLRRWHWFYFLRQKSDTCLWFSDVQGW
                     LPFGSFIQKPGQSFWLGSGYLARNHIFRVNLLVHWKRGEKEPWCLATNLPDKGLALRF
                     YRLRSWIEEMFGDFKKHGFDLEQTRLCHFQRLSRLTLTVAILYVWLVSIGTRTIHAGN
                     RHLVDRNDRRDLCIFQIGLRIIERCLTNALPFKIPLFSYR"
     gene            1003..1836
                     /locus_tag="AC16CB_04095"
                     /pseudo
     CDS             1003..1836
                     /locus_tag="AC16CB_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611865.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2128..2199)
                     /locus_tag="AC16CB_04100"
     tRNA            complement(2128..2199)
                     /locus_tag="AC16CB_04100"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2165..2167),aa:Lys,seq:ctt)
     gene            2355..3353
                     /locus_tag="AC16CB_04105"
     CDS             2355..3353
                     /locus_tag="AC16CB_04105"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005581764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HIR3341683.1"
                     /translation="MRRSLAITSIPGLRVGHAQDDEALTGVTVVLCEQGAVGGVDQRG
                     GAPGTRETDAMHPLHLVERVHAIVLSGGSAFGLDSASGVVRYLEERGVGFDVRVARVP
                     IVPAAILFDLGIGRADVRPDATMGYQACLNASANPPAEGNVGAGIGCTVGKILGITQA
                     MKSGLGSAAIEIGKGVIVAAIVAVNVFGDVINPQTGQIMAGARAITDEPLQMGAKGYF
                     ADTLQVMKTMVGRTFGFARRENTAIGVVATNAKLSKEHINKVAQMAQDGLARTVRPAH
                     TMLDGDTIFALSAGSRKADVNIVGAFAAEAFAQAVLRAVQMAQPLAGLPSALGGAL"
     gene            3356..4363
                     /locus_tag="AC16CB_04110"
     CDS             3356..4363
                     /locus_tag="AC16CB_04110"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HIR3341684.1"
                     /translation="MDTILIVEDNRDLCEALHEILSFDGYAVITASNGADALGKMQTV
                     SPDLIVSDITMPQMDGFTFFQAVRARPDWLAIPFIFLTARGERDDIIRSKYLGAEDYL
                     IKPLTQEELLTAVRARLDRSQQIRIATLRQAYETSLTVLANAIDVRDVNAQGHVERVV
                     AYSMILATELNLQGRLLDQLRLAAILHDIGKIIIQESTLFKFVSLNVTEMAEIRKHAS
                     AGAEMVRDIEYLTAIAPLIRHHHERWDGGGYPDGLSGENIPLGARIIALADSLDNMVL
                     EKPYKAALTMDQAYEEILNNSGSRYDPAVVAVFQRAWQAGKIHAVRDQWVSTKQEATQ
                     T"
     gene            complement(4373..5311)
                     /locus_tag="AC16CB_04115"
     CDS             complement(4373..5311)
                     /locus_tag="AC16CB_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020481700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3341685.1"
                     /translation="MKFRIWLALISVYITWGSTYLAIRFAVETIPPFLMAGVRFLIAG
                     TILYAWRRLSGDAPPTRVQWRSAAIVGLFLLLGGNGGVSWAEQYVPSGIAALIVGSAP
                     FWMVLIDALRPGGQRPNWQTASGVLVGFLGIALLINPSQESGGLLEMNVAGVVALLLA
                     SLLWAIGSLYSRTAQLPESPLLGTGMEMLVGSASLFLVGTLAGEWGSLDLNAITPRSL
                     WGMAYLITFGALVGFVSYTWLLRVAPTPLVATYAYVNPLIAVILGNLLAKEILTPQII
                     VAALIIVGAVAMINTGRTRALKPAGQLSVVPVSRED"
     gene            complement(5315..>6090)
                     /locus_tag="AC16CB_04120"
     CDS             complement(5315..>6090)
                     /locus_tag="AC16CB_04120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional helix-turn-helix domain-containing
                     protein/methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR3341686.1"
                     /translation="LSDVAASVNLSEYHFQRLFTHWVGISPKRFLQFLTKEHAKELLS
                     HSENILDVAYETGLSGPGRLHDLLVTTEAATPGELKSQGEGLVITYGFHPTPFGKCLL
                     ALTERGICDLVYIERDDRNEALALVKGRWPRATLQQDERQTQTVLPKIFSKLAGYETG
                     PLSLYLRGTNFQIKVWEALLRIPYGAVVSYRDIASHLGIPSAARAVGNAVANNPIPVL
                     IPCHRVIRKAGDFGAYRWGSARKKALLGWELAKNANWKE"
BASE COUNT         1544 a         1692 c         1591 g         1263 t
ORIGIN      
        1 aagaggtaac attgaggaaa aatgtgttgt caaacgctgc caattgtggg aaacgtggct
       61 ggaatacccg gagtgcagtc gcaaaaaata gcagacgaag tcggaggctc caaatcctgg
      121 ggtgtagcgc ctcagcccct acagttttac ccctcatata gtcactacta tgacttcagc
      181 ggggtacata tctggaatac gggtataaat ttaggccaag agtaacggtg gcggtgtaag
      241 ctttgtaaat tttgacactt ctcacgaaaa aagtgagaag tgtttttatt taatctagta
      301 cttcatctag ctacccgaca gttttgaatt agcggtaaga aaatagcgga attttgaaag
      361 gaagcgcatt agtcaaacac ctttcgatga tgcgtaagcc gatttgaaag atacacaaat
      421 cacggcggtc atttcggtcg accagatgac ggtttccagc gtggatcgtg cgagtaccga
      481 tagagactaa ccagacgtac aggatagcca ccgtcaaagt caaccgcgac aaacgttgga
      541 aatggcacaa ccttgtttgc tccagatcaa aaccatgttt tttaaagtca ccgaacatct
      601 cttcaatcca actacgcaag cgataaaaac gcagcgccag gcccttatcg ggcaagttgg
      661 tggccaagca ccagggttcc ttttccccgc gcttccaatg aaccagcaag ttgacccgga
      721 agatatggtt acgagccaga taaccgctgc ccaaccagaa actctgaccg ggcttctgaa
      781 taaagctacc gaagggcagc caaccctgta catcggagaa ccacagacaa gtatcggatt
      841 tctgacgcaa gaaatagaac caatgccagc gtctcagttg tttgagcacg tctaccgcac
      901 cgaattcaca gtcgcccacc aggaaaacgg cggcttttgc agggatcaag cggcgcacat
      961 aagccaacaa agccagttgc ttacgagcac tgctatgccc gcgcacatgc gggacccagg
     1021 tccaggcgat tttggaatcg tatcccggat gatgtccgtc aagatgtggt tgacctggct
     1081 ttggagcatt cagataaatc tccacggcag ttagcctggc aattcaccga ccaggaaggc
     1141 tatttcatct cagaatcaag cgtgtatcgc atcctgaagg gttacgacct gatcgaaagt
     1201 ccagccttca cagtgatcaa agccgcggac accttcaagc acccgaccac acgagtgaat
     1261 gagctgtggc aaaccgactt cacttatttc aagatcacgg ggtggggttg gtattacttg
     1321 tccaccattt tggatgatta ttcccgcttc attctggctt tcaagctgac caacactatg
     1381 tccgccctgg atgttcagga caccctgcag atagctctgg atgggaccgg cgtggagcat
     1441 gtcctggtgc agcaccgccc tcgattgttg tccgataacg gttcctgtta tgtttccgga
     1501 aaactcaaaa cctttctgga gagcaagcat atcgaccata cacgcggtgc gccctatcat
     1561 cccatgaccc agggtaagat cgagcgctat catcgttcga tgaagaacgt ggtcaagctg
     1621 cagaactatt actatccctg ggagcttgag caggagatcg cccgttttgt ggactactac
     1681 aatcatcaac gctatcacga atcgctggac aatctgaccc cggcagatgt atattttggc
     1741 agagataagg aggttttgag caagcgagag caaatcaaaa aacgtaccct gcatcaacgc
     1801 cgcctgcgcc attcacaggc gcaggcggga gtataatttc cgctagctgc gggaaaagtg
     1861 tctcttatca aattgccgtt tttgtcccaa atgttctgac gacgtacacc aagcatcact
     1921 ctggatgtgt atggacacct ctatcacgag atgcagggcg aagcggccga gatcatggat
     1981 aagcttgtta caccagttag gatcgaacta cctcaaggga ccaagcagga tgcagaacga
     2041 taaggtgcag gggaaaagaa ttgcaccaaa ttgcaccacg cgaacaaaaa gcccaccgat
     2101 ttggcaggct taccccccac atataggtga ccccgacagg actcgaacct gtaaccaatt
     2161 gattaagagt caactgctct accattgagc tacggggccg tgcgggagaa ttatacccca
     2221 aagggaagtc ttgtcaaaca aacccatggg ctcaattccg cccggagttg acggacttgt
     2281 aaggaggggg tgagcttttt gcctatataa ttatagaccg caagccaaaa cttgactgat
     2341 cagcggatgt aactttgaga cgatctttgg ccatcacctc catacctggg ctacgtgtgg
     2401 gtcacgccca ggacgatgaa gcgctcaccg gtgtcaccgt ggtgctctgt gagcagggtg
     2461 ctgtgggtgg cgtggaccaa cgcggcggcg ctcccggcac gcgggaaact gacgctatgc
     2521 acccgctcca cctggtcgag cgcgtgcacg ctatcgtgct ctcaggcggc tccgccttcg
     2581 gattagactc ggccagcggt gtggtgcgtt atctagaaga acggggagtg ggcttcgatg
     2641 tccgcgtagc ccgcgtgccg atcgtacccg cagcaatcct cttcgacctg ggcatcggtc
     2701 gcgccgatgt gcgccctgat gcaaccatgg gataccaggc ctgcctgaac gcctcagcaa
     2761 atccccccgc tgaaggcaat gtgggtgccg gtattggctg tactgtaggc aaaattttag
     2821 ggattaccca agccatgaaa tcaggcctgg gcagcgctgc catcgagatc ggcaaaggcg
     2881 tgatcgtggc cgctatcgtt gcggtgaatg tcttcggcga tgtcatcaac cctcaaaccg
     2941 ggcagatcat ggcaggcgcc agggcgatca ccgatgagcc ccttcaaatg ggtgcaaaag
     3001 gctacttcgc cgacacgctg caggtgatga aaaccatggt tgggcgaacc tttggcttcg
     3061 cccggcggga aaacacagcc ataggcgtgg tcgccaccaa cgctaagctg agcaaagagc
     3121 atatcaacaa agtcgcccag atggcacagg acgggctggc gcgcaccgta cgcccggctc
     3181 acaccatgct ggatggagac accatcttcg ccctctccgc tgggagccgt aaagcggacg
     3241 ttaatattgt gggcgctttt gctgccgagg cctttgccca ggcagtcttg cgcgccgtgc
     3301 aaatggctca accgctcgcc ggattgcctt ccgccttagg gggagcatta tgaccatgga
     3361 tactattcta atcgttgaag ataaccgaga tctgtgtgaa gctctgcatg aaatcttgag
     3421 ctttgatggc tatgcggtca tcaccgcctc caacggggcg gatgcattgg gaaaaatgca
     3481 aaccgtcagc ccagacctga tcgtatcgga tattaccatg ccccagatgg atggtttcac
     3541 ctttttccaa gccgtgcgcg cccggccgga ttggctggcg atccccttta tcttcctcac
     3601 cgctcgcggc gagcgagatg atatcatccg aagcaaatac ctgggcgcag aagattacct
     3661 tatcaagccg ctcacgcaag aagaattgct caccgcagtg cgcgccaggc tggaccgctc
     3721 tcagcaaatt cgcattgcca ccctgagaca agcctacgag accagcttaa ccgtattggc
     3781 aaacgccatt gatgtgcgcg atgtcaatgc ccaagggcac gtagaacggg tggttgccta
     3841 ctcgatgatt ttagcaactg aattgaatct gcaagggcgc ttgttggacc aattacgtct
     3901 ggcggctatt ttgcacgata tcggcaaaat catcatccag gaatcgactt tattcaaatt
     3961 tgtatccctg aacgtgacag agatggcgga aattaggaaa catgcctcgg ctggtgcaga
     4021 gatggttcga gatatcgaat accttaccgc aatcgccccg cttatacgcc accatcacga
     4081 gcgctgggac gggggtggat accctgacgg tctctctgga gagaatatcc ccctgggggc
     4141 gcgcattatc gccctggcag atagcctaga caatatggtc ctcgagaagc cttacaaggc
     4201 cgccctgacc atggatcagg cctatgagga aatcctgaac aactctggaa gccgctatga
     4261 cccagcggtg gtggcagtct ttcaaagagc ctggcaagct ggcaaaattc acgccgtgcg
     4321 tgatcaatgg gtatctacga agcaagaagc aacccaaaca taataatccc acctaatcct
     4381 ctctagatac tggtacaacg cttagctgcc ctgctggctt gagcgccctg gtgcgtccgg
     4441 tgttaatcat cgctaccgcg cccacgatga tcagcgcggc tacgatgatt tgcggggtaa
     4501 gtatttcttt tgccaacagg ttgcccaaga tcaccgcgat aagcggattc acgtacgcat
     4561 aggtagccac caggggcgtc ggggcgacgc gcagcagcca tgtataggat acaaagccca
     4621 ccagcgcccc aaaggtgatc agataagcca ttccccatag tgagcgcggc gtaatggcat
     4681 tcaagtccag gctgccccat tcgccagcca gcgtaccaac caggaacagg ctggcgctac
     4741 ctaccagcat ttccatgcca gtgccaagca gcggcgattc tggcagttgc gccgtgcggc
     4801 tataaagcga tccaatcgcc cataacaagg acgcaagcaa taacgcaacg acacccgcaa
     4861 cgttcatctc caacaaccca ccactttcct gagatgggtt aatcaatagg gcaataccca
     4921 aaaagcccac cagcacgccg ctggcagtct gccagttggg tcgctgtcca cctgggcgca
     4981 aggcatctat caacaccatc caaaacgggg ctgatcccac gatcagtgct gcgattcccg
     5041 aaggaacgta ctgttcggcc caagaaaccc caccgttccc gcctaacaac aagaaaaggc
     5101 cgacgatcgc cgctgagcgc cactgcacgc gggttggcgg agcgtctcca gaaagccggc
     5161 gccaagcata caggatagtc ccggcgatca aaaagcgcac cccagccatg agaaacggcg
     5221 ggatggtctc caccgcaaaa cggattgcaa ggtaggtaga cccccaggtg atatataccg
     5281 aaattaaagc aagccagatg cgaaacttca tccactattc cttccagttt gcattctttg
     5341 ccagctccca tccaagtaat gccttctttc gagcggaacc ccaacggtaa gcgccaaaat
     5401 cccctgcctt gcggatgaca cgatgacagg gaataagcac aggaattgga ttgtttgcga
     5461 cggcattgcc cacggctctc gcggcggagg gtatacccag gtggctggca atatcccggt
     5521 atgataccac agcgccgtac ggaatgcgca aaagagcctc ccagactttt atttgaaaat
     5581 tagtgccgcg caagtacagg ctaagcggtc ccgtctcata tcccgccaac ttcgagaaga
     5641 tcttgggcaa aacggtctgt gtctggcgct cgtcctgctg cagggttgcg cgcggccaac
     5701 ggcctttgac cagcgccagc gcctcgttac ggtcatcccg ctcaatatag accagatcgc
     5761 agatgccccg ctccgtaagg gctaacaagc acttgccaaa aggtgtggga tgaaatccat
     5821 aggtgatgac caacccctcg ccctggctct tcaactcgcc gggggtcgcc gcctcagtgg
     5881 tgacgagcag gtcgtgcagc cgccccgggc cagacaggcc agtctcataa gccacatcca
     5941 agatgttttc cgaatgggac aaaagctcct tggcatgttc tttggtcaga aattgcaaaa
     6001 aacgcttcgg actgatgccc acccagtggg tgaacaacct ttgaaagtgg tactcgctca
     6061 ggttaacact ggcagccacg tcggacaggc
//
LOCUS       DBNGSX010000123         4410 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_424658, whole genome shotgun sequence.
ACCESSION   DBNGSX010000123 DBNGSX010000000
VERSION     DBNGSX010000123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_424658"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(451..942)
                     /locus_tag="AC16CB_04125"
     CDS             complement(451..942)
                     /locus_tag="AC16CB_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341687.1"
                     /translation="MKHSTIIWFALFALVLAACSPAQAPVPSDIQPTNQPSSVLPSTL
                     QPAVKDLTRSDSQGAVVVEIKPLNLDNPADTLLFEVSLNTHSVDLSMDLAALATLTTD
                     TGHTVEAAHWDAPRGGHHVSGTLSFPATWDGAPLLEGASMLTLSIQNLDASLRMFNWN
                     LSH"
     gene            complement(942..1436)
                     /locus_tag="AC16CB_04130"
     CDS             complement(942..1436)
                     /locus_tag="AC16CB_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341688.1"
                     /translation="MTLSRRIIWSVGAGLAGMLFLSSLFFGVVSWAGGLEHAASLFWQ
                     DRRFIVPIILGFGIQTGLYTILKKQLFLPTAKVGPSGVLTGTGGATSTAAMVACCAHH
                     VTDVLPILGLTAAATFLAQYRTMFMLVGLGTTLVGVAVMLFILYTERRKVLKMSAKAA
                     LPEA"
     gene            1669..2124
                     /locus_tag="AC16CB_04135"
     CDS             1669..2124
                     /locus_tag="AC16CB_04135"
                     /inference="COORDINATES: protein motif:HMM:NF025170.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCYCGC motif-containing (lipo)protein"
                     /protein_id="HIR3341689.1"
                     /translation="MKRTVYKTLLPLMVILMIFSGSLSACSRDGGAENEHDLAMRPLD
                     ELPAEVKSAPVVVQQAYQFAAANPEIMTHIPCYCGCGEMGHASNYACYVSGVGEGGEI
                     QYDPHALGCSICVDITQDTMRLLQQGKTVPEIKAYVDQTYAKYGPSNMP"
     gene            2175..2606
                     /locus_tag="AC16CB_04140"
     CDS             2175..2606
                     /locus_tag="AC16CB_04140"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR3341690.1"
                     /translation="MFFWTIILALGLLIWWVPVPSALAGAPSERVIRIQASRFAYSPH
                     VMKVNRGDRVTFELEATDVVHGLSIDSYGLETFAEPGRTARLTFDADRVGTFRFHCTV
                     TCGNMHPFMTGKIQVGNDYVMWRVVSLAALVMVAGVWGWRR"
     gene            2603..4027
                     /locus_tag="AC16CB_04145"
     CDS             2603..4027
                     /locus_tag="AC16CB_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024211.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR3341691.1"
                     /translation="MNTLKELRWGKKAVEGRPQSTVQRLISQPGRTNLLRLPLMRFLL
                     ESRWPITLARGVTLAGFVFTLTAGLLGPRVGSHNFAIIFVWIAWWTALKLAFIPLGGR
                     SWCSVCPIPLPGEWLQQGGVLERGSRRLGLNLRWPKRLKGSWLQSGGFLLIGLFSAIT
                     LTDPRVTAWVLLALFGLAIALSLVFERRAFCSHICPIGGFTGLYAKTAPLEVRVVDTK
                     LCTTHSEKTCYQACPWGLYPVALRDAAACGLCMECLRACPRDNIAVNLRPFGSDLGAG
                     RSGIRLDDTFLALVMLGSALVFAAVFTGPWGALKSAAYAIGTSAWWGYVLGFLTLNLV
                     LLPGLFSLAVWSGHQASALKTPLKRLLADRSQVLLPLGLTAWIAFTVSFAFPKASYVL
                     AVLSDPFGWGWNLLGAASSIKLPDVTGLSQLLQTVLLLGGLFWSTRVAGRLASAEERS
                     LRQEVPLLAFCLVFTLVMFWLLIG"
     gene            4031..>4410
                     /locus_tag="AC16CB_04150"
     CDS             4031..>4410
                     /locus_tag="AC16CB_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012344.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR3341692.1"
                     /translation="MSREWLARILIISLVVAALAVPAWGWWQRSQGVLIHGRMAETGG
                     WSPENLTAEVGKPLKLRLTSDDVMHGFAVGQMAMQPVDMLPGEISEIVLNFERPGKYV
                     FYCSRWCSVNHWRMRGTIEVSGAA"
BASE COUNT          905 a         1144 c         1320 g         1041 t
ORIGIN      
        1 ggatcgacca gcgtggtgca gccctgctgg gcgctggctg aagaaacgtg gggactctca
       61 tccagcgatg acaagacagg ccaagtgaat accaacagca aggctgaaat caccaggaca
      121 ataacaaacc agtaacgatt cttggtcggc atggcgatac ctcacaaaca tttcggattt
      181 tgccgcgttg ttctatctgg aaatgcaacc ccccatggga gtgaacgatg tggagcaatt
      241 ataccttatt gccctaaaaa gtatgatata agacttgtgt ttcctctagt tctgccttga
      301 tgatgcgctg gcagttggat taccaatctg tcttggatat agagcggggt taattggaat
      361 attgaaaatc tgactatata tggctttgca ccaaggaatt atccgatact cacattaaat
      421 aaaaaattgg gcgaggagta aaaaagcacc ttaatgcgat aaattccagt tgaacatccg
      481 taaggaagca tccaggtttt ggatgctcag ggtgagcatg ctggcacctt cgagcaatgg
      541 agccccatcc caggttgccg gaaaagagag cgtgcctgag acgtgatgtc caccgcgtgg
      601 cgcatcccag tgcgcagctt caaccgtatg ccccgtatct gtggtcaatg tggccagggc
      661 ggctaggtcc atgctcaggt cgactgagtg ggtgttcagg ctcacttcaa aaaggagggt
      721 gtctgcagga ttatccaggt taagcggctt gatctcaacc accaccgcgc cctggctgtc
      781 tgagcgagtt agatccttga ccgcgggttg caatgtgctg ggcaacacac tggacggctg
      841 attggttggc tgaatatccg agggaacagg tgcctgtgct gggctgcaag ctgccagcac
      901 aagcgcgaac aaggcaaacc aaattattgt ggaatgtttc attatgcctc cggcagggcg
      961 gctttcgctg acattttgag gaccttacgg cgttctgtgt aaaggatgaa caacatcaca
     1021 gctacaccga ccagggtggt tcccaatccg accagcatga acattgtgcg atactgggca
     1081 agaaaagtcg ctgccgccgt aagcccaagg attgggagta catcggtgac gtggtgggcg
     1141 cagcaggcca ccatggcggc tgtggatgtg gctccgcctg tccccgtgag cactcccgaa
     1201 ggtcccacct tggcagtggg caggaaaagc tgctttttaa ggatggtata gagtcctgtc
     1261 tggattccaa atcccagaat gatcgggaca atgaatctcc gatcttgcca gaacaggctg
     1321 gcggcgtgtt ccaatcctcc tgcccaggag acaacgccaa agaacaggct gctcagaaaa
     1381 agcatcccgg ctaggccggc tcctaccgac cagataatgc gtctcgataa tgtcatggcg
     1441 aagagctcct gtaatgttcg aatacaacgc gtccgagtta atcgtatcgg tttgggcgtt
     1501 tttggtcatg cgctactcag ctattcttgg agtaggcaat agtgcctata cgcgcccccg
     1561 tcagattgcg ctgactgtca cgaagcaggt tccttataat gggtgcagat tgaagaaaac
     1621 ctatggcaga ttttgcgcct gcaataggat gagaggagct cgcgcttcgt gaaacgcacc
     1681 gtgtataaaa cccttctacc cctgatggta atattgatga ttttttccgg ttcgctaagc
     1741 gcttgcagcc gcgatggcgg cgcggagaac gagcatgacc tggcgatgag accgctggat
     1801 gagctgccgg cggaagtcaa aagcgcacca gtcgttgtgc agcaggctta tcaatttgct
     1861 gcagcaaatc ctgagatcat gacccacatc ccctgctact gcgggtgtgg cgagatgggg
     1921 cacgcgtcca attacgcctg ctacgtttcc ggcgtgggtg aaggagggga gatccagtac
     1981 gacccgcacg cattgggctg ctcaatctgt gtggatatca ctcaagatac aatgcgcctg
     2041 ctccagcaag gaaaaaccgt gcccgagatt aaagcttacg tggaccagac ttacgcaaag
     2101 tacggtccat cgaatatgcc ataacctgac gattgagaga tagtgccaaa ggatctggag
     2161 tgtctctgcg gactgtgttt ttttggacta taatccttgc gcttggcctg cttatttggt
     2221 gggtacctgt gccatccgcc ttagcaggcg cgccctcgga gcgggtaatc cgcatccagg
     2281 caagccgttt tgcctattcg ccgcacgtga tgaaggtcaa ccgcggcgac cgggtgactt
     2341 ttgagctgga ggcgaccgat gtggtccatg gcttgtctat tgatagctat gggctggaaa
     2401 catttgccga gccgggcagg actgctcgac tgacctttga tgcggatcgg gtgggcacgt
     2461 tccgcttcca ttgcacggtg acttgcggga atatgcaccc ctttatgaca ggtaaaatcc
     2521 aggtgggcaa tgactatgtg atgtggcggg tggttagcct ggcggctctg gtgatggtag
     2581 ccggggtatg ggggtggcga agatgaacac gctcaaagag cttcgctggg gtaaaaaagc
     2641 ggtagagggt cgcccacagt ccactgtgca gcgtcttatc tctcaaccag ggcgcacaaa
     2701 tcttttacgc ctgcccttga tgcgctttct attggagagc cgctggccga tcaccctggc
     2761 gcgtggggtc actttagcag gcttcgtctt caccctgact gctggcctgc tcggtccccg
     2821 ggtaggcagt cacaactttg ccattatctt tgtatggatt gcctggtgga cagcgctcaa
     2881 actggctttc atcccattgg gtgggcgttc ctggtgcagt gtttgcccga tcccgctgcc
     2941 gggagaatgg ctgcagcagg gaggcgtgct ggagcgaggc agccggcggt tagggctgaa
     3001 cctgcgctgg cctaagcgct tgaaaggctc ctggctgcag tccggcggtt tcttgctgat
     3061 cgggctgttc agcgccatca cgctcaccga cccgagagtg accgcctggg tgctcctggc
     3121 gcttttcggg ctggcgattg ctctctcctt agtctttgag cggagagcct tctgtagtca
     3181 tatctgccca attggcggtt tcactggact gtatgccaag accgcgcccc tggaagtgcg
     3241 cgttgtggat acgaagttgt gtaccaccca cagcgagaag acctgctacc aagcctgccc
     3301 ctggggcttg tatccagttg cgctgcggga tgccgctgcc tgcggtctgt gcatggaatg
     3361 ccttagagcg tgcccgcgcg acaatattgc tgtcaacctg cgcccgtttg gcagcgacct
     3421 gggtgcgggt cgatcgggca tccgactgga cgatactttt ctagccctgg taatgcttgg
     3481 ttcagcgctg gtctttgcag cggtgtttac cggaccgtgg ggcgcgctca agtcggctgc
     3541 ctacgcaatc ggcaccagcg cctggtgggg ttacgtcctg ggtttcttga cactcaacct
     3601 ggtgttgctg cctggactgt tcagcctggc agtttggagc ggacatcaag cctcggccct
     3661 caagacaccc ttgaagcgcc tgctcgctga tcgttcccag gtgctgcttc cactcggctt
     3721 gaccgcctgg atcgccttta ccgtgtcctt cgcgttcccc aaggcttcct atgtgctggc
     3781 agtgctatct gaccccttcg gctggggatg gaatttattg ggtgcagcca gttctatcaa
     3841 gctgccggac gttactggct tgagccagtt gttgcaaaca gttctgttat taggtggctt
     3901 gttctggtcg acgcgggttg ccggtcgctt ggcttcggca gaagagcgat ccttgcgtca
     3961 agaagtgccc ttgctggcct tctgcctggt ttttacgctg gtcatgttct ggctgctgat
     4021 tggataagcg atgagtcgag agtggcttgc ccgtatcctg attatctcgc tggtcgttgc
     4081 agcactggcg gtgccggcct ggggctggtg gcagcgcagc caaggcgtgc tcatccatgg
     4141 gcggatggcc gaaaccggcg gttggtcgcc agagaaccta accgctgagg tgggcaagcc
     4201 gctcaagctg cgcctcacat ctgatgacgt gatgcacggc ttcgccgtgg ggcagatggc
     4261 gatgcaaccg gtggatatgt tgcccggtga gatcagcgaa atcgtgctga attttgagcg
     4321 accgggaaaa tacgttttct actgctcgcg ctggtgcagc gtcaatcact ggcgcatgcg
     4381 cgggacgatt gaagtaagcg gagcggcgca
//
LOCUS       DBNGSX010000124         5511 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1186748, whole genome shotgun sequence.
ACCESSION   DBNGSX010000124 DBNGSX010000000
VERSION     DBNGSX010000124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5511)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1186748"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1294)
                     /locus_tag="AC16CB_04155"
     CDS             complement(<1..1294)
                     /locus_tag="AC16CB_04155"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019344 - L-cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase"
                     /protein_id="HIR3341693.1"
                     /translation="MSTNGLSTSDTLRVYEDILQTIGNTPLVHLTRLGRGLSCSVYAK
                     VEFFNPGGSVKDRIALNIIDEAERSGRLKPGGLVVEATSGNTGVGLAIACAIKGYRTV
                     FVMPDKMSQEKIQFLRAFGAKVVITPTAVEPDDPRSYYSVARRIVSESPNAILANQYH
                     NPENPRSHYLTTGPEIWEQTQGRVTDIVIAMGTGGTITGVGRYLKEKNPAIRIVGVDP
                     AGSILLETWQNGGRIPEDARAAPYKVEGFGEDFLPSTLDLSLVDEVIRVTDKESFLWT
                     RRLVKEEGIFSGGSSGAAVAAAIRYARTLTPERVVVVLLPDSGSRYLSKVFDDKWMRE
                     NGYLEATWGEVTLDDVLESKSIRQLITANQQDRIVDVIALMKKHDISQVPVLNGDDTL
                     VGMVAEVDLLKYMLESGVTHSPDESIAAIVQPARAVFPV"
     gene            complement(1458..2306)
                     /locus_tag="AC16CB_04160"
     CDS             complement(1458..2306)
                     /locus_tag="AC16CB_04160"
                     /inference="COORDINATES: protein motif:HMM:NF014679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR3341694.1"
                     /translation="MSKVALVTDSTAYIPKDLVDQYSITVAPQILIWGDKTYLDGVDI
                     TPEEFYTRLTNTTVMPTTSQVNIASFQQIFKQLIDEDYEVLAVLLSKHFSKTIDSALQ
                     AREMFPDAPIEIVDSTTTAMAMGFIVLSVARLAEQGASLAECKALAEKATNHVGVLLT
                     VDTLEFLHRGGRIGGGQRFLGSALSIKPILEVRDGRIEPVERVRTRKKSLLRLIELVK
                     ERIGEREPVRLATLHANAHDEAKEIHEEAKTQLKIVESVFSELSPVVGTHAGPGTVGL
                     AFMAGM"
     gene            2551..3345
                     /locus_tag="AC16CB_04165"
     CDS             2551..3345
                     /locus_tag="AC16CB_04165"
                     /inference="COORDINATES: protein motif:HMM:NF013757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR3341695.1"
                     /translation="MKPVSTPTRSRYNIEALERGLDILALFTSDTPSLSLTQIVEILN
                     INKSTAYRVLSTLEGLNYLERDPITRLYRPGLKVLQLGFTAISNLDVREVAHPFLVKL
                     SQDLGETVSLAVLDGLYTVYVDRVRNQAIVGVVLGVGSRLPAHCSSLGKVLLSDLPTD
                     ELERRLAQAELTSFAPNTISNQEDLISELSKVREQGYAIGDEELAVGLRAAAAPIRDI
                     NHRIVAAINATGATINISYERLNNEIIPALIETAHQISITLGYTPN"
     gene            3366..4577
                     /locus_tag="AC16CB_04170"
     CDS             3366..4577
                     /locus_tag="AC16CB_04170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3341696.1"
                     /translation="MDFNLSEEHLQVQKVVHDFCQKEVAPIIKEADRTQTMHPSILPR
                     MAELGILGICIPEKYGGQGFDYITLGLVCEELEAMDTHLRVVMSVHMGLNSMALLQWG
                     TEEQKQKFLVPQARGEKYAMFGLTEPGVGSDVANMASTARRDGDSYIINGEKMWISLA
                     TRAHNILWVARTNPERPDPHDQLSAFIIETDRPGISVGDIHGKLGVRAGSTGWVSCQD
                     VRVPASNRLGEEGEGFKIAMSCLDNGRYTVGAGAVGLIRASLEASVKYAKERKAFGKE
                     ISKHQLVQQKIATMVRDYEIGRLQYLQVGWMKNMGMRNTRETSLLKWFATDASFEAAH
                     QAIQIHGAYGYSDEYDVERYLRNARGAIIYEGTSEIHQLIQAGYALGYRKDSKLRCEL
                     PAYDEKEWRTS"
     gene            4585..>5511
                     /locus_tag="AC16CB_04175"
     CDS             4585..>5511
                     /locus_tag="AC16CB_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3341697.1"
                     /translation="MSPSALAGLRVIDFSRVLAGPYCTMLLADLGADVIKVEHPQGGD
                     DTRQWGPPWLDGESAYFLSVNRNKRSLTLNLKHQKGRELAHQLAISADILVENFKVGT
                     MAKYGLDYETLSQQNPGVIYCSITGYGQNGPDYDRAGYDFIVQAEAGLMSIIGPPEGP
                     PYKVGVAIVDITAGMFASHAILAALYSRQQTGRGQMIDIALLDSQIAWLANVAQNYLV
                     SGQAPKRYGNAHPNIVPYEVFQTSDGYLALGVGNDKQYQRLCDVAGRTDLWDDTRFQT
                     IPGRVTFRDELIPKLQEVFLSRPTATWIEQLSA"
BASE COUNT         1377 a         1458 c         1405 g         1271 t
ORIGIN      
        1 caaccggaaa aaccgcccgg gcgggctgga caattgcggc aatgctctca tcaggagaat
       61 gcgtaacacc cgactccagc atgtatttga gtaaatccac ctcggcgacc atgcctacca
      121 aagtgtcatc tccattcaat accggcactt gggagatatc gtgctttttc atcagagcaa
      181 tcacatccac gatgcggtct tgctggttcg ccgtaataag ctggcgtata gacttagact
      241 ccagcacgtc gtctaaggtg acctcacccc atgtcgcctc aagataacca ttttcgcgca
      301 tccatttgtc atcaaacacc ttggataggt aacgcgagcc agaatcgggc agcaatacca
      361 ccactacacg ctcaggtgtg agagtccggg cgtagcgaat tgcagctgcc actgccgctc
      421 ccgaggaacc accgctgaaa ataccctctt cttttaccaa gcggcgcgtc cacaagaagg
      481 actccttgtc cgtgacacgg atcacttcat ctaccaaaga cagatccaat gtcgaaggca
      541 gaaagtcttc cccaaaaccc tcaaccttat aaggtgcagc ccttgcatct tcgggaattc
      601 tgcctccgtt ctgccaggtc tccagcaaga tcgagccggc tggatctact ccgacgattc
      661 ggatcgccgg gtttttctct ttcagataac gtcccactcc tgtgattgtg ccaccagtgc
      721 ccatggcgat cactatatcg gtcacgcgcc cctgggtctg ctcccagatt tccggaccag
      781 tggtcagata atgactgcga gggttctctg gattgtggta ctggttggcc aggatagcat
      841 tgggggattc agatacaatc cggcgggcta cactgtaata cgagcgcgga tcatctggct
      901 cgacagcagt gggggtaatg accactttgg caccaaaggc gcgcagaaac tggattttct
      961 cctggctcat cttgtcgggc atgacgaata ccgtgcggta acccttgatg gcacaggcaa
     1021 tggctaagcc tacacccgta ttaccagatg tggcttccac gaccaatccg ccgggtttga
     1081 gccggccgct acgctcagcc tcatcgatga tattcaaggc tatgcggtct ttgaccgatc
     1141 cacccgggtt gaaaaattcg accttggcgt acacggaaca ggataacccc cgtccaaggc
     1201 gagtcaaatg aacgagggga gtattgccga tcgtttgcag gatatcttca tatacccgta
     1261 atgtgtcgga agttgagaga ccatttgtgc tcatatttcc atccattaag atcaaaaata
     1321 ttggtttatt tgttaattac ttctaagcgt accagaagta tattcaagcg acaagggttg
     1381 caaaataaaa aaatatggtg cagccactgg ctgagcagcg gactacacca tttgaatcgt
     1441 tgacctaggg ttgatggcta catgcctgcc atgaatgcca gaccaaccgt acccggacca
     1501 gcgtgggtgc cgaccacagg gctgagttca gagaatacgc tttctacgat cttcaattgc
     1561 gtcttggctt cttcatgtat ttccttggct tcatcgtggg cattggcatg cagagtcgcc
     1621 agacgcacag gctccctctc accgatccgc tccttgacca actcgatcag gcgaaggagt
     1681 gattttttgc gcgtccgcac tctttccacg ggttcgatac gcccgtcacg tacctccagg
     1741 ataggcttta tactcaaggc tgaacccagg aaacgctgcc caccgcctat tctccccccc
     1801 cgatgcaaga attcgagggt atccacagtt aagagcacac ccacatggtt agtggctttc
     1861 tctgctaagg ccttgcattc cgccaggctg gcaccctgct ctgccaggcg tgccaccgag
     1921 agcacaataa agcccatggc catggcagta gtggtagagt caacaatctc aatcggtgca
     1981 tcaggaaaca tctcgcgtgc ttgcagggct gaatcgatcg ttttcgaaaa gtgtttggaa
     2041 agcaggactg ccaatacttc ataatcctcg tcgatgagct gcttgaagat ctgctggaaa
     2101 gaggcgatgt taacctgcga ggtggtgggc atcactgttg tatttgtcaa gcgggtatag
     2161 aactcttctg gggtgatatc aacgccatcc aggtaagttt tgtcccccca tattaagatc
     2221 tgtggtgcaa ccgttatact atattgatca acaaggtctt tggggatgta ggcagtgctg
     2281 tcagtgacta gcgctacttt tgacatattt cctcctccgg caaatatatg catttgcgta
     2341 ttactattca atcctctata acccatatta catggcaaag ttacgcgaga aataccatgc
     2401 attcggctac ctgggttatt agaattaggg aaagtatcaa gatgaagggt ttacggaaaa
     2461 cgaattccaa ttttcgaaag gcggatatct tgacaagggt gtgtattgat gctaatatga
     2521 attttattca gcaatacttg gtttcacata atgaaacccg tctcaactcc cacgagatcg
     2581 cgatataaca tagaagccct ggagcgcggg ctggatatcc tcgcgctctt cacctcagat
     2641 accccctccc tttccttaac tcaaattgtt gaaatcctga acatcaataa gagcacggct
     2701 taccgcgtgc tctccacctt ggaagggctc aactatctgg agcgagatcc aattacccgt
     2761 ctctaccggc ctggtttaaa agttctccag cttgggttca cagccatcag caacctcgat
     2821 gtgcgtgagg tggctcaccc gttcctggtg aaattgtcgc aagatttggg tgaaacagtg
     2881 agcctggcag tattggacgg tctttatacg gtctatgtgg atcgcgttcg caaccaggcc
     2941 attgtagggg tagttttggg ggtggggtcc cggctaccag ctcattgctc atccttgggt
     3001 aaggtgctgc tcagtgacct gcccaccgac gaattggaga ggcgcctcgc tcaagctgaa
     3061 ttgacatctt tcgcccccaa cacgatcagt aaccaggagg acttaataag cgaattgtcc
     3121 aaagtgaggg aacaaggcta tgccatagga gacgaggaac tggcggttgg attgcgtgcc
     3181 gccgcagccc ctattcggga tatcaatcac cggattgtgg cagccatcaa cgcaactggg
     3241 gcaaccatca acattagtta tgaacgcctc aacaatgaaa tcataccggc tctcatagag
     3301 accgctcatc aaatatccat taccctaggg tacacaccaa attgaagcat aaaaaggatt
     3361 aagctatgga tttcaatctc tctgaagaac atctacaagt gcaaaaggtt gtccacgact
     3421 tctgccaaaa agaagtcgca ccgattatca aagaggctga ccgcacccaa accatgcatc
     3481 ccagcattct accgcgcatg gcagaactgg gcatcttggg catctgcata cccgaaaaat
     3541 atggcgggca aggtttcgat tacatcacgc ttggcctggt ttgcgaggaa ttagaagcca
     3601 tggataccca tttgcgggtt gtgatgtctg tgcacatggg actgaacagc atggcgcttc
     3661 tccagtgggg caccgaggag caaaaacaaa aattcctcgt gccacaggct cgcggtgaga
     3721 agtatgccat gttcggactc accgagccgg gcgtgggcag cgacgtggca aacatggcgt
     3781 ccaccgctcg ccgcgatgga gattcttaca tcatcaacgg tgagaagatg tggatttcac
     3841 tggctaccag agcccacaat atcttatggg tggcgcgaac caaccctgag cgaccagatc
     3901 cgcatgacca gctctccgct tttattatcg aaaccgaccg accgggtatt tcggttgggg
     3961 atatccacgg caagcttggc gtgcgtgcgg gctccaccgg ctgggtaagc tgccaggatg
     4021 tacgcgtgcc cgccagcaat cgcctgggcg aagaaggaga aggcttcaaa atcgccatgt
     4081 cctgcctgga taacggtcgt tataccgtgg gagccggagc agtcggcttg atccgtgcca
     4141 gcctggaagc cagcgtgaaa tatgccaagg agcgcaaagc ctttggtaaa gagatcagca
     4201 agcaccagct tgtacagcag aaaattgcca cgatggtgcg ggattatgaa atcggacgac
     4261 tgcaatacct gcaggtaggc tggatgaaaa acatgggtat gcgcaacacg cgcgagacat
     4321 ctctgctcaa gtggtttgcc accgacgcct cgttcgaggc cgcccatcag gccatacaaa
     4381 tccatggcgc ctatggatac agcgatgaat atgatgttga acgctatctg cgcaacgcac
     4441 gcggcgccat aatttatgaa ggcaccagtg aaatccacca actcatacag gctggctatg
     4501 ctctaggcta ccgtaaggat agcaagctgc gctgtgaact acctgcttac gacgaaaaag
     4561 agtggcgaac ttcgtgagta ttcaatgagc ccatcagcgc tggctggttt acgtgtgatt
     4621 gacttctcaa gggtgctggc aggtccctac tgcaccatgc tcttagccga cctcggggct
     4681 gatgtaatca aggttgagca tccgcagggc ggtgatgaca cacgccaatg gggacctccc
     4741 tggttggatg gcgagagcgc ctatttcctg agcgtgaacc gcaacaaacg cagcctgaca
     4801 ctgaacctga aacaccaaaa agggcgagag ctagctcacc agttggcgat cagcgcagat
     4861 attttggtgg aaaacttcaa agtgggcaca atggcgaagt atgggctgga ttatgaaacc
     4921 ctgagccagc aaaatccagg ggtaatatat tgcagcatca ccggctatgg gcaaaatggg
     4981 ccagactatg accgtgccgg gtatgatttt atcgtccagg ctgaggcagg gctgatgtcc
     5041 atcattggcc caccagaagg gcccccgtat aaagttggtg tggcgattgt ggatataacc
     5101 gccggcatgt tcgccagtca cgccatcctg gctgcgctgt acagccgcca acaaactggc
     5161 cgcggtcaaa tgattgatat cgccttgctg gattctcaga tcgcctggtt agccaatgta
     5221 gcccaaaatt accttgtctc tggtcaggct ccaaagcgct atggcaatgc ccaccccaac
     5281 attgtcccct atgaagtctt tcagacatca gatggctacc tagcccttgg cgtaggaaac
     5341 gataaacaat atcaacggct gtgtgatgtc gctggacgga ctgacctgtg ggatgatacc
     5401 cgctttcaga ccattccagg gcgggttact tttcgcgatg agttgatacc gaagttacag
     5461 gaagtcttcc tgagccgccc caccgctacc tggatcgaac agctcagcgc a
//
LOCUS       DBNGSX010000125         9277 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_633211, whole genome shotgun sequence.
ACCESSION   DBNGSX010000125 DBNGSX010000000
VERSION     DBNGSX010000125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9277)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9277)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9277
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_633211"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..1049)
                     /locus_tag="AC16CB_04180"
     CDS             complement(99..1049)
                     /locus_tag="AC16CB_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIR3341698.1"
                     /translation="MRYRILVASRSFGSTSSKPWDVLNQAGCEILQADMTHKMTEERL
                     IELLQGVDGAIIGVVPMTAYVLENAPNLKVVSMHGVGVDHIDLTAAAQKGIVIANCLG
                     ANDQSVADLTIGLMISIARDIPSVDRVVRGGGWGAYEGHELWNKTLGLIGLGRIGRGV
                     AKRALGFDMKVLAYDPYVQPENVEQDISLVSFDKVLREADYISLHATLSEETRHMIGA
                     AQLQAMKPTAYLINTARGALVDEEALYTALINKQISGAALDVYNNEPPKGSPLLQLEN
                     VVVTPHIGAHTRESIERVGVMAAENVLRTLQGGQPHYRVV"
     gene            complement(1046..1696)
                     /locus_tag="AC16CB_04185"
     CDS             complement(1046..1696)
                     /locus_tag="AC16CB_04185"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - (R,S)-4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HIR3341699.1"
                     /translation="MKSHFEILSKLLAERAIAIVRLDSGEKLVKVAEALKAGGLSAIE
                     FTFSTPGALDMMKEASAHFGGEVLLGAGTVLDPETARAAILAGAEFIVTPTVNLDTIG
                     ICKRYGKPIVAGAMTPSEMLKVWEAGADLVKVFPASTIGGPDYIRAVLAPLPQPRLVP
                     TGGVSADNAAQFLKAGAVAVAVGGNLVDKKAVARGDWAAITAEAQRLVAAIGRVAA"
     gene            complement(1780..2757)
                     /gene="iolN"
                     /locus_tag="AC16CB_04190"
     CDS             complement(1780..2757)
                     /gene="iolN"
                     /locus_tag="AC16CB_04190"
                     /EC_number="3.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007933246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-scyllo-inosose hydrolase"
                     /protein_id="HIR3341700.1"
                     /translation="MSKYNWQIPPSGGHMDNPDGIYFQNMTMLEVEERLKKNDLIIIP
                     VGSSEAHGPHACYGEDTFLVTRMAEAVAKQTGCTVSQPIWFGSHPYHHIGMPGTIPVD
                     EDTFTAYLSSVIAGFWNTGFRKQILLNGHGQEYVIPTAIHRFAKRYQVPSILINLNWY
                     YAIQPYVKDKAHGGPFETPFVHADEAETSFSLGLFPEMIHMQDAVDTEFHGFLPAGHV
                     DSAANAYQRAIPWYGHVGLGTIEIIGNPEGVVGKATLASVDKARPGLEALLDYMVKLH
                     DDIMERFPVGKLPDANLVTQRFTQEQVDELIKGPLHGGKHLYTVAWPPI"
     gene            complement(2772..3839)
                     /locus_tag="AC16CB_04195"
     CDS             complement(2772..3839)
                     /locus_tag="AC16CB_04195"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF028222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR3341701.1"
                     /translation="MGIPKTMKAAVLFDYGDLRVTEVPVPTLEVGEVLVRVHSVAICG
                     SDPAIIAKGWKGYSKLGEFIPGHEFTGTAVALAPDVTELREGDRVAVEPHKGCYRCEN
                     CLRGLYTTCLNYGNHEKGHRHYGFSSNGGYAEYAACHVNTLHKIPESIAFEEGAILTG
                     AGTVLYGYERIGWVRPGETVVVTGPGSIGLISAHIAKILGAGRVILTGTRDDRLAVGR
                     QMGVDITLNINEVNVREKIMELTKGIGADMVVECTGQPGPAAEALEIVRKNGRISYNG
                     IYHEQVTLQLDKIVQWNLLITGPKAEGMLNLERAIPLMADGRMNLKPLITHTFSLDDI
                     NEAFDTFTGRVGGAIKVIVQP"
     gene            complement(3861..4895)
                     /locus_tag="AC16CB_04200"
     CDS             complement(3861..4895)
                     /locus_tag="AC16CB_04200"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR3341702.1"
                     /translation="MRALVKTVKGPGNLELREVPVPQIGADEALIRVRACGICGSDLK
                     IQDDQHPYTPPVVIGHEFAGEIAEIGAGVSGWQVGDRVVSEQHIGACGHCRQCLTGNA
                     FACSAKRAPGYFTDGAFTEFIKVPAWLLHRIPEKLSFVEAAFTEPSAVAAHGMLDRTG
                     INPEDVVVILGCGPIGLVAAKMAQEEGASKVIVTGIDRDEIARLPKGREIGIDHVVNV
                     MKEDLAGLVNNLTHGEGADVVIELSGALPAINQAFQLARRLGRVGIIGQPPADEIKIP
                     YRQAMFRALTVSFSYSSKYTSWERALSLFERGAINPSQFITHVLPLEAWEQGFELSRS
                     GQAVKVVLEP"
     gene            complement(4905..5963)
                     /locus_tag="AC16CB_04205"
     CDS             complement(4905..5963)
                     /locus_tag="AC16CB_04205"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF028222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR3341703.1"
                     /translation="MRAVVFTRPDTFELSDVPDPTPDANGVIVSVKATTICATDVKIL
                     HGTVPFVKFPHIPGHEWGGEIVEVGSNVKGLRKGDLIGVEAHVGCAKCERCLEGLYNL
                     CENYGHPETGHAHIGFTVPGGLAEYCAIPARAAYVMPEGLHADHAAFTDTVGIVLWAF
                     ARAGGVHGGETVAVVGPGALGLLSVQVARALGAGRLIVVGTALDRQRLEMARQMGADE
                     IVIAQQDINPAQVVRDLTDGKGVDLVVEFAGSAEAGRQSLEMARRGGRVVLGGATSPG
                     RELNIDLSIIVRGHLNIFGSVANPQSISRRANVLMQKGLVDVTPLITHQMPLTDFSKA
                     WKMFLEREGDPIRILMHP"
     gene            complement(5978..7201)
                     /locus_tag="AC16CB_04210"
     CDS             complement(5978..7201)
                     /locus_tag="AC16CB_04210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reductive dehalogenase"
                     /protein_id="HIR3341704.1"
                     /translation="MPKNFLSRLYVKQLPPDNPPYQVNESYQRFNQRDNLTVGRPSWD
                     EEFKPLLRKSVEVRVKKIKLGKSNYKLEDYSLFDAGGVSVFRMGNAINHSNRGVTSWS
                     SLGNKLPPGVEAWQGSPEKAAEMVKRVARYFGASLVGIVPLNKQYIYSHAWWKDGTHK
                     EIVFADVDSPKESEEQLIISEKMRWVIVMGIPMDPQMMDYTPSPLGCAETRITYSRMG
                     LIVSGVAEFLRGIGYNAIPSINDLGLNIPMAIDAGFGEQGRNGKLVTPEFGPSLRLCK
                     IVTDLPMERDNPIHFGVTEFCEVCKKCADECPAHAISTGERTWSRDSISCSQGHYTWH
                     LDNEACRKYWDLGVAANCTVCVRACPFTKQPGLVHEVARTFVSNFPVMDPLLKKMDDW
                     LGYGQEKDGSRFWDG"
     gene            complement(7203..7655)
                     /locus_tag="AC16CB_04215"
     CDS             complement(7203..7655)
                     /locus_tag="AC16CB_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3341705.1"
                     /translation="MILNVNGNNYEVDARTSTLLEVLRDELGLLGTKEGCSIGICGAC
                     TVLVDGKPHSSCLVLAEQVAGKEILTIEGLGGVETLHPVQQAFLKHGAFQCAYCTPGF
                     VLTTVALLNENPSPTEDEIREYLSGNLCRCGSYQNILQAVRTASQGGG"
     gene            complement(7652..>9277)
                     /locus_tag="AC16CB_04220"
     CDS             complement(7652..>9277)
                     /locus_tag="AC16CB_04220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3341706.1"
                     /translation="GNKLTIWTGSQAPYLVRRVVAGILGIEQESVRVIVPPLGGGYGG
                     KGHIRIEPIVAAMARKTGGRPVKFVLSRAEEFVTVTKHAASITLKTGVRRDGTFTARQ
                     VTIYWNGGAYADASPMLVPQGMVRSVGPYRIPAVHVDSYGIYTNLPPAGAYRGAMSSQ
                     TTWAYESQMDIIAHKMGWDPLEFRLKNLFISGDHFATGEELHDVHFIECLEASAKPLG
                     AKPEDSTRRIGRGYGVMMKSTISTSKSQARLRLADDGKVTLFTSTVEMGQGGHTAMAQ
                     ITAEALGLPVEMVSVVGPDTDVTPFDSTTSASRSTNMMGNAVMAAGAAVSRKLIELAA
                     PYLETPPEELRLENGHVVAEDTSISFADVLKRNGLDSLEESGEYATAFKMDSETGQGV
                     STPHWHQGAGAAEVEVNTETGRVTILRYSAASFAGKVVNPHLAQMQNDGNVVYGLGPA
                     MMEEIVFDGGQPTNPNLADYPIPSIRDVPPELISICIESEEGEMHGIGEMTLPPVAPA
                     IANAIEDAVGVRIKDLPITAEKVLRALREKPGL"
BASE COUNT         1995 a         2809 c         2354 g         2119 t
ORIGIN      
        1 gcctgtgcat caatgatgac gctcttgcaa ctgcctgtcc ctaggtcgat gcctaagaac
       61 caatcggtgc gtgcgttttc agacattcct gtttttattc acaccactct atagtggggc
      121 tgaccacctt gtagagtccg cagcacgttt tcagctgcca tcacgcccac acgctcgatt
      181 gattcgcgcg tatgcgcgcc gatatgcggc gtaacgacca cattctccaa ttgcagcaag
      241 ggactgcctt tcggtggctc gttgttgtac acatccaggg ctgcccccga aatttgctta
      301 ttgataagtg cggtgtacag cgcctcctca tcgaccaacg cgccgcgtgc ggtgttgatc
      361 agataagcgg ttggcttcat ggcttgcagt tgcgccgcgc caatcatgtg tcgcgtctct
      421 tcacttagcg tggcgtgaag ggaaatatag tcggcttccc tgagaacctt atcaaagctc
      481 accaacgaga tatcctgctc cacattctct ggttgtacat aaggatcgta agcgagcacc
      541 ttcatatcaa atcccagtgc ccgtttggct acgccgcggc cgatccgccc caagccaatt
      601 aagccgagcg ttttattcca caactcatgc ccctcgtatg ctccccatcc gcctccccgc
      661 acaaccctgt ctacagacgg gatgtcgcga gcgatggaga tcatcagccc gatcgtcagg
      721 tctgccacag actggtcgtt tgcgcccagg cagtttgcga tcacgatgcc cttttgggct
      781 gctgcggtta aatcgatgtg atctacgccc acaccgtgca tactgaccac ttttagattg
      841 ggcgcgtttt ccagcacata ggcggtcatg ggtaccacac caatgatagc cccgtctaca
      901 ccttgcagca gttcgatcaa gcgttcctcg gtcatcttgt gtgtcatgtc tgcttggaga
      961 atttcacagc ctgcctggtt cagcacatcc cagggtttag aagacgtgct cccgaatgaa
     1021 cggcttgcca ccaaaattct atacctcatg cagcaaccct cccaatggct gctaccagtc
     1081 gctgcgcttc ggcggtgatc gcggcccagt cgcccctcgc cacggctttc ttgtcgacta
     1141 gattgccgcc caccgcgaca gcaactgccc ctgccttgag gaactgggct gcgttatccg
     1201 cgctgacacc acctgtcggc accagccgcg gttgcggaag gggagcgagc accgccctga
     1261 tatagtctgg ccccccgatg gtgctggcag ggaaaacctt cacaaggtca gcacccgcct
     1321 cccaaacctt cagcatttca gagggcgtca tggcgccagc cacgatgggt ttgccatagc
     1381 gcttgcaaat cccaatcgtg tcgaggttga cggtcggtgt gacgatgaac tccgctcctg
     1441 cgaggatcgc cgcccgcgct gtttcggggt cgagcacagt gcctgcaccc aggagcactt
     1501 ccccaccgaa atgagctgac gcttctttca tcatgtcaag cgcgcctggc gtggagaagg
     1561 tgaactcaat agcagataac ccgcctgcct tgagcgcctc ggctaccttg accagttttt
     1621 ctccactatc cagtctaacg atagcgatcg ctctctccgc gagcagttta ctcaagattt
     1681 caaaatggga tttcattcag aatgcctccc caggcgatgc gcagtcacag gatattaagc
     1741 gaaaacttgc ctatcgatcc gcttaaccaa aacacacgct caaataggtg gccaggcgac
     1801 tgtgtagaga tgtttaccgc cgtgaagcgg gcctttgata agctcgtcca cctgctcttg
     1861 cgtaaagcgt tgggtgacga gattggcgtc gggcaattta ccaacgggga agcgttccat
     1921 gatatcgtcg tgcaatttga ccatgtaatc gagcagggcc tcaaggccag ggcgtgcctt
     1981 atcaacgctg gcgagcgtgg ctttgcctac cacaccttca ggattgccga ttatttctat
     2041 ggtgccgagg ccgacatgcc cgtaccaggg gatggcgcgc tgataggcat tggctgccga
     2101 gtcgacatgc cccgctggca ggaagccgtg gaactcggta tccaccgcat cctgcatgtg
     2161 gatcatctca gggaataagc caagcgagaa ggatgtctcg gcttcgtcgg catggacgaa
     2221 gggtgtctcg aatggccctc catgcgcctt gtctttgaca taaggttgga tggcgtaata
     2281 ccagttgaga ttgatcaaaa tggaaggcac ctgatacctt tttgcaaagc ggtgaatagc
     2341 agtcgggatg acgtattcct gcccgtggcc attgagcagg atttgcttgc ggaagcccgt
     2401 gttccagaat cctgcaatga ctgagctcaa gtaggcggtg aatgtatctt cgtctacagg
     2461 tatcgttcca ggcatcccga tatggtgata ggggtgactg ccaaaccaga tgggttgcga
     2521 aaccgtgcag cctgtctgct tggcgaccgc ttccgccatg cgcgtgacga ggaacgtatc
     2581 ttcgccatag caggcgtgcg gcccgtgtgc ttctgaacta ccgacaggaa tgatgatgag
     2641 gtcgtttttc ttcaaacgct cctcgacctc cagcatggtc atgttctgaa aatagatgcc
     2701 atcggggtta tccatatgcc cgccgcttgg cgggatctgc caattgtatt tgctcatagt
     2761 tatctccatt ctcaaggttg aacaatcact ttgatcgcgc cgcccacgcg accagtaaac
     2821 gtatcgaaag cttcgttgat atcatccaaa ctgaaggtgt gtgtgataag cggtttcagg
     2881 ttcatgcgac catctgccat caacgggatc gcccgctcta ggttcaacat cccctcggct
     2941 tttggcccag taataagtaa gttccactga acgattttat ccagttggag ggtcacttgc
     3001 tcgtgataga tgccattata ggaaattcgc ccgttcttgc ggacaatctc cagcgcttct
     3061 gccgccggcc caggttgccc ggtgcactcg acgaccatat ctgcgccaat acccttggtc
     3121 agttccatga ttttctcacg aacattgacc tcgttgatgt taagggtgat atcaacgccc
     3181 atttggcgac caacagccaa acggtcgtcg cgcgtgccag taaggatgac acgccccgcc
     3241 cccaggattt ttgcgatgtg agcagagatc agtccgatcg agcctggtcc ggtcacaact
     3301 accgtctccc ccggcctgac ccagccaatg cgttcataac cgtataacac cgtcccagcc
     3361 cctgtcagaa tagcaccctc ttcaaaagcg atgctttccg ggatcttgtg cagggtgtta
     3421 acgtggcagg ctgcatactc ggcgtagccg ccattggaac taaagccata atggcggtgg
     3481 cctttttcgt ggttgccata gttcaggcag gtggtgtaca ggccgcgcag acagttctcg
     3541 cagcgataac agcccttgtg tggctctact gccacccggt ccccttcacg gagttcggtc
     3601 acatccggcg cgagggctac cgctgtgccc gtgaactcat ggccggggat gaactcgcct
     3661 aatttagagt atcccttcca gcctttggca atgatggctg gatcgctgcc acagatggca
     3721 accgaatgga ctctgaccag cacctcgccc acttccaggg tgggcacagg gacttcggtg
     3781 acccgcaaat caccatagtc gaacaacact gcggctttca tagtctttgg gatgcccatt
     3841 gtttctcctc tttcttattc tcaaggttcc agcacaacct tcacagcctg gccacttcga
     3901 gatagctcga agccttgctc ccaagcctcc agcggcaaga catgtgtaat aaactgcgaa
     3961 ggattgatcg cgccgcgctc gaagagggac agcgctcttt cccaactcgt gtatttggaa
     4021 ctgtagctga atgataccgt caacgcccga aacatagcct ggcgataggg gattttgatc
     4081 tcatctgcag gtggctggcc gatgatgccc acccgtccca ggcgacgcgc cagctgaaaa
     4141 gcctgattaa tcgctggaag ggcgcctgac agctcgatga ccacatccgc gccctcgcca
     4201 tgtgtcaggt tattcaccag ccctgccaag tcttccttca tcacattgac cacatggtct
     4261 atgcctattt cacgcccttt aggcagacgg gcgatttcat cgcggtcaat gcccgttacg
     4321 atcacttttg aggcgccctc ctcctgtgcc atcttagctg ccaccaggcc gatgggacca
     4381 cagcccaaaa tcacgacaac atcttcaggg ttgattcccg tccgatctag catgccatgc
     4441 gcggcgacag ccgagggctc ggtaaatgcc gcctcgacaa aacttaattt ctcagggatg
     4501 cgatgtaata accacgcagg gaccttgatg aattccgtaa acgccccatc cgtaaaatag
     4561 cctggggcac gcttggcact gcacgcaaaa gcgttgcccg tcaggcactg ccgacaatgc
     4621 ccacaggctc caatatgctg ttccgagacc acgcggtcgc caacctgcca gcccgaaaca
     4681 cccgcgccaa tctcggcaat ttcgcctgcg aactcatgcc cgatcacaac aggtggcgta
     4741 tacgggtgct ggtcatcctg gatcttgaga tcgctgccgc aaattccgca cgctcgcacg
     4801 cggatcaagg cttcgtctgc gccgatctgc ggaacgggaa cttcacgcag ctctaaattt
     4861 cctggcccct taaccgtctt caccaaagcg cgcatttagc ctccttatgg gtgcatcaag
     4921 atacggatcg gatcgccttc gcgctccaga aacatcttcc aggctttcga aaaatcagtc
     4981 aatggcattt ggtgtgtgat caatggtgtt acatccacca agcctttttg catcaacaca
     5041 ttcgcgcggc gtgaaatgct ttgcggattg gcaactgagc cgaagatgtt caagtgaccg
     5101 cgcacgatga tgctcaaatc gatattcaac tcacgcccag ggctggttgc accgccgagg
     5161 acaacgcgcc caccccgccg cgccatttcc agtgactggc gacctgcttc ggcagaacca
     5221 gcgaattcga ccaccaggtc aacacctttg ccatctgtca agtcacgcac aacttgggca
     5281 ggattgatat cttgttgcgc tatgacaatc tcatccgcgc ccatctgccg cgccatctcc
     5341 aatcgctggc ggtctaacgc agtgcccaca acaatcaacc tgccagcccc cagcgcgcgc
     5401 gccacctgta ccgatagcaa tcccagcgct ccaggaccaa cgacggccac agtctcgccg
     5461 ccgtgcactc ccccggcgcg cgcaaaagcc caaagcacta taccgaccgt gtcagtgaaa
     5521 gccgcgtgat cggcgtgcaa gccttcgggc atcacatacg cagcgcgtgc tgggatagca
     5581 caatattcag ccaatccacc tggcacggta aacccgatgt gcgcgtgacc tgtttcagga
     5641 tgtccgtaat tttcgcaaag gttatagaga ccttccaggc agcgctcgca tttggcgcag
     5701 ccgacatgcg cttctacgcc aataagatcg cctttacgca agccttttac attggacccg
     5761 acctccacaa tttcaccgcc ccattcgtgg ccagggatgt gtggaaattt tacaaatggg
     5821 actgtgccgt gcagtatctt gacatccgtc gcacaaatag tggtggcttt aacggaaaca
     5881 atcaccccgt tagcgtctgg tgtggggtca ggaacatcgc tgagttcaaa tgtatcaggg
     5941 cgggtaaaga caaccgcgcg catgactcat cccaccctta tccatcccag aaacgggagc
     6001 catctttctc ttgaccatat ccaagccaat catccatctt tttcagcaac gggtccatca
     6061 cagggaaatt ggagacgaat gtgcgcgcca cttcatgcac cagtccaggt tgcttggtga
     6121 acgggcaagc ccgcacgcaa accgtgcaat tggccgctac acccaggtcc cagtatttgc
     6181 ggcaagcctc attatcgaga tgccaggtgt aatgaccctg gctgcatgat atgctgtcac
     6241 gcgaccaagt gcgctcaccc gtcgagatgg cgtgggcggg acactcgtcg gcgcacttct
     6301 tacaaacctc gcagaattcc gtcacaccaa agtggatcgg gttgtcccgt tccatcggca
     6361 ggtcggtgac aatcttgcac aggcgcaggc tgggaccgaa ctctggcgtg acaagcttcc
     6421 cattgcgacc ttgctcgcca aagcctgcgt caatcgccat cgggatgttc agccccagat
     6481 cgttgatcga cgggatggcg ttgtaaccga tgccgcgcaa gaattccgct acacctgaaa
     6541 cgatcaatcc catgcggcta tatgtgatac gcgtctcagc acagcccagc ggcgaagggg
     6601 tgtaatccat catctggggg tccatcggga ttcccatgac gatcacccag cgcatctttt
     6661 ctgagatgat caactgctct tcgctttctt tgggcgagtc cacgtctgcg aagacgatct
     6721 ctttgtgcgt gccatccttc caccaggcgt gcgagtagat gtactgtttg ttcaggggca
     6781 cgatgcccac cagcgaggcg ccaaaatagc gcgccactcg cttgaccatt tcagctgctt
     6841 tctcgggcga gccctgccag gcctccaccc caggcggtag cttgttgccc agcgaggacc
     6901 aagatgtcac gccacgattg gaatggttga tagcattacc catgcgaaac acgctcacgc
     6961 cacccgcgtc gaacaggctg tagtcctcca gcttgtaatt ggacttgccc aacttgatct
     7021 ttttaacccg cacttcaacg gattttctga gcaatggctt gaattcttca tcccagctcg
     7081 ggcgaccaac cgtcaggttg tcacgctggt tgaagcgctg gtagctctca tttacctgat
     7141 acggcgggtt gtcgggcggc aattgcttga catacaatcg agacaaaaaa ttcttcggca
     7201 tctcatcctc ctccctggct tgccgttctt accgcctgca aaatattctg gtatgatcca
     7261 cagcggcata gattgcccga taaatattcg cggatttcat cttcggttgg cgaggggttt
     7321 tcgttcagca aagccaccgt ggttagcaca aaccctggtg tgcagtatgc gcattgaaac
     7381 gccccatgct tcaaaaacgc ttgctgcaca ggatgcaaag tctccacgcc tcccaatccc
     7441 tctatggtca ggatttcctt acctgcaacc tgctcagcca gcactagaca gctgctgtgc
     7501 ggcttgccgt ccaccagcac agtgcaggct ccgcagatgc caatactgca accttcttta
     7561 gtccccaaca aacccagctc atcgcgcagg acttcgagca gcgtgctcgt tcttgcatca
     7621 acctcgtaat tattcccgtt tacattcagg atcatagccc aggcttctcc cttagcgccc
     7681 gcagcacttt ctcggctgtg atgggcaaat ccttgatgcg taccccaacc gcatcctcga
     7741 tcgcgtttgc aattgcaggt gcaactggcg gcagggtcat ttcacctatg ccgtgcatct
     7801 caccctcttc ggattcgata cagatgctga tcaattcggg tgggacatcg cggattgatg
     7861 ggatcgggta atctgccaga ttaggattgg taggctgacc accgtcgaaa acgatctcct
     7921 ccatcatggc aggaccaaga ccgtagacca cattaccatc attttgcatt tgtgccaggt
     7981 gtggattgac caccttacca gcgaaggatg ccgcagaata gcgcaaaatc gttacacgcc
     8041 ccgtttcggt attaacttcc acctcggctg cgcctgctcc ttggtgccag tgtggcgtgg
     8101 acacgccctg tcccgtctca gaatccatct tgaatgccgt ggcatattct cctgactctt
     8161 ccagagagtc gagaccatta cgcttgagca catccgcaaa tgaaatggat gtgtcttcgg
     8221 ctacaacatg gccgttttcc agtcgcagtt cttcaggtgg cgtttccaaa taaggtgcag
     8281 ccagctcgat gagtttcctg gacacggcag cccccgctgc catgacggcg ttgcccatca
     8341 tgttggtcga gcggctagcg gaagtggtcg aatcgaacgg tgtcacgtcg gtgtcggggc
     8401 cgaccacgct caccatttcg acaggcagcc caagcgcctc tgcagtgatc tgtgccatgg
     8461 cggtatgccc accctggccc atctcgactg tactggtaaa aagcgtaacc tttccatcat
     8521 ccgcgagtcg caagcgcgcc tgcgatttgg aggtcgaaat cgtgctcttc atcatcacgc
     8581 cgtagccgcg accgattcga cgtgtggaat cttctggctt cgcgcccaat ggtttggctg
     8641 acgcctccaa acactcgata aagtgcacat catgcaactc ttcgcccgtg gcaaaatgat
     8701 cgccagagat gaacaagttc ttcaggcgga actccagcgg gtcccagccc attttgtggg
     8761 cgataatatc catttgggat tcataagccc aggtggtctg cgaactcatt gcgccgcgat
     8821 aggcgcctgc aggcggcaga ttggtgtaaa taccatacga atccacatgc actgctggga
     8881 tgcggtatgg cccgacagag cgcaccatgc cttgaggtac gagcatcggg cttgcgtccg
     8941 catacgcgcc gccattccag taaatcgtca cctgccgcgc cgtaaaggtg ccatcccgtc
     9001 tcacgcccgt tttcagcgtt atggaggcgg catgtttcgt caccgtgaca aactcctcag
     9061 cgcgcgaaag cacaaacttg acaggccgtc cgcccgtctt acgtgccatg gctgccacaa
     9121 tcggctcaat gcggatgtgt cccttcccgc catagcctcc acccaacggc gggacgatca
     9181 cgcgcaccga ttcctgttcg atgccaagga tgcctgccac cacgcgccgc accaggtacg
     9241 gggcctgtga accagtccat atggtcagct tattgcc
//
LOCUS       DBNGSX010000126         1988 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1325754, whole genome shotgun sequence.
ACCESSION   DBNGSX010000126 DBNGSX010000000
VERSION     DBNGSX010000126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1325754"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="AC16CB_04225"
     CDS             <1..986
                     /locus_tag="AC16CB_04225"
                     /inference="COORDINATES: protein motif:HMM:NF018012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR3341707.1"
                     /translation="LSSLHDLDASWNNTVKHIKTETLLGEVATRYAIEMATVVAGSRE
                     ALRENPLLSVLICTIAPLVQDKEGIEGAMLLAEEGIPVSFMAMPTMGTTAPVTQAGAF
                     VVADAEIISATVLMQLINPGTPVSHSLLQGWVDPRNGNYIAYPVDARVRYGPIDMSHH
                     WGMPSFGGAVGTESVEPGTWQSAADVSLDPLLISLASCEWATGIGLNRTFTLLHPEAI
                     ILDDEIYHRARYALAEEEVSPETLALDVIDKVGPGGHYLSQKHTRKYIRTTVRLGLTH
                     VTDQTGKYRHPLEVAREKFDWILQNHQPPPLEADKQAELNKILASAEKEIV"
     gene            1022..>1988
                     /locus_tag="AC16CB_04230"
     CDS             1022..>1988
                     /locus_tag="AC16CB_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaminase"
                     /protein_id="HIR3341708.1"
                     /translation="MDGQKTPINRDRLRALIAQEQQRFVAEHPASKKLFEKAKAHLLD
                     GVPMNWMVEWAGTFPIFVKEGSGAYLWDVDGHRYLDLCLGDTGAMTGHSPHAAVQAVT
                     DRLGRGITFMLPTEDAVWLGEELHRRFGLPYWQIALTATDANRFTIRLARYITGRKRI
                     LVFNGCYHGTVDESFVTIVDGKPVSREANAGPPVDPTLTTRVIEFNDIPALEAALQPG
                     DVACVLAEPALTNIGIVLPEPGYHAALREITHRTGTLLIIDETHTISAGPGGYTQAHH
                     LQPDMLTLGKPIGSGVPTAVYGFSAEVADRISRAIQNEGETIHGVG"
BASE COUNT          463 a          559 c          537 g          429 t
ORIGIN      
        1 aattatcctc tttgcacgat ttggacgcat cctggaacaa tacggtcaag cacattaaaa
       61 ccgagacgct gctgggagag gtcgccactc gctatgccat cgagatggct acagttgtcg
      121 cagggagtag agaggcgcta cgggaaaacc cgttgttatc cgtgctaata tgcaccattg
      181 cgcctttggt gcaagataaa gaagggattg agggcgccat gctcctggct gaggaaggca
      241 ttcccgtgag tttcatggct atgcctacta tgggcacgac tgcacccgtt acacaagccg
      301 gtgcgtttgt ggttgcagat gctgagatca tcagcgcaac ggtgctcatg cagctgatca
      361 atccgggcac accagttagc cactccttgt tacaaggctg ggttgacccg cgaaatggca
      421 actacatcgc ctatccagtc gatgctcgcg ttcgttacgg gcccatagac atgtctcatc
      481 attggggtat gccttcgttc gggggcgctg ttggaactga gagcgttgaa ccagggacct
      541 ggcaatcggc cgccgatgtg tctctggacc cattgttgat cagtttggca tcttgtgagt
      601 gggcaaccgg tattggtcta aaccgcacct tcaccctgct ccatccagag gcaatcatcc
      661 tggatgatga gatctatcat cgagctcgct acgccctggc agaggaggag gtatcacctg
      721 aaacacttgc actggatgtg atcgacaagg tcggtcccgg cgggcattat ctgtctcaaa
      781 aacacactcg caagtatatc cgcactaccg taagactcgg tcttacccat gtcaccgatc
      841 aaaccgggaa atatcgccat ccactcgaag tcgctcgtga aaagttcgac tggatcttgc
      901 agaaccacca accccctccc cttgaagctg acaagcaagc cgagctgaac aaaattctcg
      961 cctctgcaga aaaagagatc gtctaggtgg caactcaaaa ccaaattggg gattgaaagg
     1021 catggatggg caaaaaacac caatcaatag ggacagatta agagccctca ttgcccagga
     1081 acaacagcgc ttcgtggcag aacatcctgc ctcgaagaag cttttcgaga aggcaaaagc
     1141 tcatttgctg gatggcgtgc ccatgaattg gatggtcgag tgggctggga cgtttcccat
     1201 tttcgtcaag gaaggatcag gggcttatct atgggatgtc gatggccatc gttatctcga
     1261 cctgtgcctg ggcgacacag gtgctatgac aggccattcg ccgcatgctg ctgtccaggc
     1321 cgtcaccgat cgcctgggcc gcggaatcac cttcatgctg cctaccgagg atgccgtctg
     1381 gttgggagaa gagctgcacc gtcgcttcgg tttgccatac tggcaaatcg ccttgaccgc
     1441 cacagacgcc aatcgcttca caatccgctt agcgcgttac atcaccggac gcaaacgcat
     1501 attggtgttc aatggttgct atcacggcac ggtggacgag tcgttcgtca ccatcgtgga
     1561 tgggaagccc gtatcgcgcg aagccaatgc aggcccgcca gtggatccca ccctgaccac
     1621 gcgggtgatc gagttcaatg atatccccgc cctcgaagct gccctccagc ccggcgacgt
     1681 ggcctgcgtg ctggccgagc ccgccctgac caacatcgga attgtgcttc ccgaaccagg
     1741 ttatcacgcg gctttacggg agatcacaca ccgcacaggg acattattga tcatcgatga
     1801 aacgcacacc atctctgccg gtcctggtgg gtacacgcag gctcatcatc ttcaaccaga
     1861 tatgctcacc cttgggaaac ctatcggtag cggtgtgccc actgcggtat acggcttctc
     1921 ggccgaagta gctgatcgca tctccagggc catacaaaac gaaggcgaga ccatccacgg
     1981 tgtgggtg
//
LOCUS       DBNGSX010000127         2877 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_79267, whole genome shotgun sequence.
ACCESSION   DBNGSX010000127 DBNGSX010000000
VERSION     DBNGSX010000127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_79267"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="AC16CB_04235"
     CDS             <1..214
                     /locus_tag="AC16CB_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004472686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HIR3341709.1"
                     /translation="IPMTAHILGGCPFGRDDQEGVIGLDFQVHNYPGLFIVDGSVVPA
                     NPGINPTLTITALSEYAMSQMPPSSS"
     gene            230..1216
                     /locus_tag="AC16CB_04240"
     CDS             230..1216
                     /locus_tag="AC16CB_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR3341710.1"
                     /translation="MTVLSKSSLHLILIITFIVLFLAACGPETSAPAPTQMEQLAPTQ
                     TQPSMPSRTIPPPAVTEAPAEPSNESTPLPTQIPAVEVLPAEPQEIEFQAEDGQSLIG
                     RYYPSAVSPAPLVVLMHWAPGDQNDWSAIATWLQNREINASTPGGKSWLDSSWFPAML
                     EGQSFAVFTFTFRNCEGGCKSFDRAAWLLDAQSAMRIARGLKGVNTNRIATIGASIGA
                     DGAHDGCAWLNTQFPNSCLGALSLSPGSYLTIPYKDVVSALGSEQPSRPAWCLYAEGD
                     AESVQACEAASGDNYRSVKYSGNHHGMMLVQPELEPSVLQIMLVFLKLAFSL"
     gene            1308..1685
                     /locus_tag="AC16CB_04245"
     CDS             1308..1685
                     /locus_tag="AC16CB_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341711.1"
                     /translation="MSEKITNQLDQLANYMAQRDVLSLQKKELIDQVLTPEIKARLEE
                     IEAEFAGKLETVDANINALEEEIRQMALQHGASVRGTFLQVVWHKGRVSWDTRSLDEY
                     AARHPEISAFRKQGEPYVSILKR"
     gene            1689..2018
                     /locus_tag="AC16CB_04250"
     CDS             1689..2018
                     /locus_tag="AC16CB_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3341712.1"
                     /translation="MSYINLNDIEEREMIPGYRARFVHSEHMTLAYWTIKAGSSLPEH
                     SHPHEQVVNLLEGEFEFSLAGEIRRMRPGMVVDIQPNVSHAGKAITDCRILDVFYPVR
                     EDYRLSK"
     gene            complement(1990..2484)
                     /locus_tag="AC16CB_04255"
     CDS             complement(1990..2484)
                     /locus_tag="AC16CB_04255"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3341713.1"
                     /translation="MFTLRPATSDDEAIIRDLVNAARINPTGLDWRRFIVATLSGGEV
                     IGCGQVKPHKDGSSELASIVVASHWRGNGVASAIIEHLVAANPGPLYLTCRSGLGPFY
                     EKFGFRVVSESEMTPYFRRLIRLIKAIGFVALGSEGVLVMLRNSEAGSGNSENIILKG
                     DSLL"
     gene            complement(2527..>2877)
                     /locus_tag="AC16CB_04260"
     CDS             complement(2527..>2877)
                     /locus_tag="AC16CB_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3341714.1"
                     /translation="AMQIILANFEITEDETILPRLSFDNHMQIVRAMWEFKTYERFSR
                     LNCPVLLVPARPTEPLSPQESQHLAFKERGVEEAMKRIQDVRVQWMEDAIHDIPLQRP
                     AELAARLADFAASL"
BASE COUNT          708 a          811 c          757 g          601 t
ORIGIN      
        1 catccccatg accgctcaca ttttgggtgg ctgccccttt ggacgagatg atcaggaggg
       61 cgtgattggt ctggacttcc aagtccataa ttaccctgga ctctttatcg tggatggatc
      121 ggttgtgcct gccaatccag gcatcaaccc caccctgacc atcaccgcgc tgtctgaata
      181 tgccatgagc cagatgcctc ccagctcctc ctaaagggag gtaaaagcaa tgacagtttt
      241 atccaaatca tccctacatc tgatattaat cataaccttc atcgtcttat tcttagcagc
      301 ctgcgggccg gagacaagcg cccccgcgcc gacacagatg gagcagctcg cccccaccca
      361 aacgcagcca agcatgccct cgcgcaccat ccctccccca gcagtgacag aagcaccagc
      421 cgagccatca aatgaaagca ctcccctacc gacgcaaata cctgctgtcg aagtactgcc
      481 agcggagccg caggagatcg aatttcaggc cgaagatggt caatcgctta tcggccgtta
      541 ttacccatcg gcggtatccc ctgccccgct agtggtattg atgcactggg cccccggaga
      601 tcaaaacgac tggtctgcca ttgctacctg gttgcagaat cgcgaaatca atgcctcgac
      661 gcccggaggg aaatcttggc tggactcgtc ctggtttccc gccatgctgg aggggcagtc
      721 tttcgcagtg ttcactttca cattccgcaa ctgcgaagga gggtgcaaat cgttcgatcg
      781 cgccgcatgg ctattggacg ctcaatcggc tatgcgcata gcacgcgggc tgaaaggtgt
      841 aaacaccaat cgaatcgcca ccatcggagc cagcatcggg gccgacggag cgcacgacgg
      901 atgtgcctgg ctgaacaccc aatttcccaa tagttgtctg ggggcactct cgctctcgcc
      961 gggcagctac ctgacaattc cctacaaaga cgtggtgagc gctctggggt cggagcagcc
     1021 atccaggcca gcctggtgtt tgtatgccga aggtgacgcg gagtcagtgc aggcttgcga
     1081 agcggcctca ggtgacaact accggagcgt gaaatacagc gggaaccacc acggcatgat
     1141 gcttgtccag cctgaattgg agcccagtgt attacaaatt atgctggttt ttcttaagct
     1201 tgcatttagc ctataggcag atttcccatt gtttcactcc cccatccccc ttgtcccctc
     1261 tccccaggaa aaggggatac aatcaaacct tggcaaccga tgaacccatg agcgaaaaga
     1321 ttaccaatca attagatcaa ttggccaact atatggcgca gcgcgatgtc ctgagcctgc
     1381 agaaaaaaga gctgatcgac caggtgctga ctccggaaat caaggcacgc ctggaggaga
     1441 tcgaagccga gttcgccggt aagctagaaa ccgtcgatgc aaatatcaac gcattggaag
     1501 aagaaattcg ccagatggcg ctccaacatg gcgcgtcggt gagaggcacc ttcttgcaag
     1561 ttgtctggca taaggggcgc gtctcttggg atacaagatc actagatgaa tacgcagcga
     1621 gacatccgga gatcagcgcc ttccggaaac aaggtgagcc atacgtgagc attcttaaga
     1681 ggtgagacat gtcctacatt aatctcaatg acattgaaga gcgagaaatg atccccggat
     1741 accgggcgcg ctttgtgcat tcggagcaca tgaccctagc ctattggacg atcaaagccg
     1801 gatcgtccct gccggagcac agccacccac atgagcaggt tgtcaatcta ctcgaaggtg
     1861 agtttgagtt tagcttagcc ggcgagatcc ggcgcatgag accaggcatg gtagtggata
     1921 tccaacccaa tgtaagccat gctggcaaag caatcaccga ttgccgtatc ctagatgtgt
     1981 tttatccggt cagagaagac tatcgccttt caaaatgata ttttccgaat tgcctgaccc
     2041 ggcttcggaa ttgcgcagca tcaccagcac cccctcggaa cccaatgcaa caaagccaat
     2101 cgccttaatc aggcggatga gccggcgaaa gtaaggggtc atctcggact cactaaccac
     2161 ccggaaaccg aatttctcgt agaatggtcc caatccactt cggcaagtca ggtagagcgg
     2221 gccggggtta gcagccacca gatgttctat gatggcgctc gcgacaccgt tgccgcgcca
     2281 gtgcgatgcg acaacaattg aggccagctc gctcgagcca tccttgtggg gtttaacctg
     2341 accacagccg atgacctccc caccagacaa agtggcgaca ataaagcgcc gccagtccag
     2401 accggtaggg ttgatccggg cagcgttgac taggtctcgg atgatcgcct catcgtcaga
     2461 ggtagcaggg cggagggtga acataggata taacatgcgc taacaatcaa atttgcatgg
     2521 accaacctac agggatgcag caaaatctgc cagtcgcgca gccagctcag ccgggcgctg
     2581 tagagggatg tcgtggatag catcttccat ccactgcacg cgcacatcct ggatacgttt
     2641 catcgcctct tctaccccgc gctctttgaa agccaggtgt tgactctctt ggggagagag
     2701 cggctctgtt gggcgtgcag gcaccaacag cacagggcaa ttcaagcgag agaagcgttc
     2761 gtaggttttg aattcccaca tggcgcgcac aatctgcatg tggttatcga agcttaggcg
     2821 tgggagaata gtttcatctt cagtgatttc aaaattcgcc aggataattt gcatggc
//
LOCUS       DBNGSX010000128         6744 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_79305, whole genome shotgun sequence.
ACCESSION   DBNGSX010000128 DBNGSX010000000
VERSION     DBNGSX010000128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6744)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6744)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6744
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_79305"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="AC16CB_04265"
     CDS             complement(<1..602)
                     /locus_tag="AC16CB_04265"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR3341715.1"
                     /translation="MNHKIFEASEALMGLDKAVAVARPDPPGRLLFVDNIRTMLTIQV
                     LLFHLMIIYAGTGSWIYKEGGEDFITGMIGAYFVVVSQAYFMGLFLLISAYFVPGAYD
                     RKGSKKFLKDRLVRLGIPLAFYSWIISPLLVYLDLRVTEGVTLPWWDYFPGLKHEAFI
                     GSGPLWFVETLLIFTLLYALRRRLARPVQGITAPASSFPS"
     gene            complement(599..1117)
                     /locus_tag="AC16CB_04270"
     CDS             complement(599..1117)
                     /locus_tag="AC16CB_04270"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3341716.1"
                     /translation="MKIPQLATSRLTLRAFTDDDVVPFHRMLIEKDVLRYFPNTDPPS
                     LERVQKMVSGILKHWAEHGFGLWAVERRQSGEFMGRCGLQVIPDTGEVEVDFILGKAF
                     WGQGYATEGGQVSVQYGFERLGVEMIVGIVHPENMASQRVLEKLGLVFREKTQYFGMD
                     CFRYAVENQGEP"
     gene            complement(1351..1965)
                     /gene="tmk"
                     /locus_tag="AC16CB_04275"
     CDS             complement(1351..1965)
                     /gene="tmk"
                     /locus_tag="AC16CB_04275"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR3341717.1"
                     /translation="MFITLEGPDGSGKSTQAGLLVNFLRQRGSSVLATREPGGTAIGD
                     QVRSVLSNLENTAMHPRTEILLFQASRAQLVEQVIRPQLARRGIVVCDRYADSTLAYQ
                     GYGHQVDMVRLRAIVEFATGGLKPDLTLLLDIDVEEGLRRRTKSGEWNRLDAYDLAFH
                     QRVRQGYFQLAQDEPQRWVKIDAAQPPVMVQADIQRVVLERLEA"
     gene            complement(2059..3678)
                     /locus_tag="AC16CB_04280"
     CDS             complement(2059..3678)
                     /locus_tag="AC16CB_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HIR3341718.1"
                     /translation="MSQRRITDDLQALLDVLPQSISQAVSDANDSDNLLEVILDLGRV
                     PTARFVDREVVLSPTEVPHKDIDYVVERIGAFDADNRAGLERTLHRISAIRNRRGHIV
                     GLTCRVGRAVYGTTDIIKDLIEGEKSLLLLGRPGVGKTTMLREAARLLAESKRVVIVD
                     TSNEIGGDGDVPHPAVGRARRMQVSTPSLQHEVMIEAVENHNPEAIIIDEIGRELEAA
                     AARTIAERGVQLIGTAHGNTLENLLLNPTLSDLVGGIESVTLSDEEARRRGTQKTVLE
                     RRSPPTFDVLIEIQTRQRLAVHEDVAASVDALLRGFPLSPEIRSRDESGSIQIEKPVQ
                     PARAYSQQQGLRRQHTNGGRAYSPQAIRDEYPAESQPPAYTAEDRASRGPLQTVRIYA
                     YGVARNRLSQAARRLSVPVVLVNDPGQADVLVTLRSYYRKRQRTVVDAENRGMPIYVL
                     RANTINQMLQFLMGLFNLAEEQPDEMGEDRIRQETMAAISAVLNGERWVELSPASSGV
                     RRLQHQMARQANLLSHSYGKEPRRRVRIFRE"
     gene            complement(3684..4667)
                     /locus_tag="AC16CB_04285"
     CDS             complement(3684..4667)
                     /locus_tag="AC16CB_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341719.1"
                     /translation="MNIRPFDWRDLPALHRYRQQSVFLHSSLVLTRGPMLISSALLSS
                     LAPNMGISTSVSVEEGDQDQKLIGQSLHSLGSQCAQLTFLTPDGALDSDNLSSLLEHL
                     SKQAVERGAFRLLADVDENTQAFEALHQAGFAIYARQRIWQLKGSPTMPSVSAGWRHA
                     SDRDLIPVRSLYANIIPGLVQQIEPFPAERLNGLIYRQGDELLAYVELKYGHRGIWAQ
                     PFVDPDLKDVTSRLAELLSNLPNRLSRPVYLCVRSYQAWLESTIEELGAEPSQRQAMM
                     VKHLAIPQKALRAVSLPNLEGGHPEVTAPIRQTQAVSPQNGGRAAAQLENH"
     gene            complement(4752..5348)
                     /gene="pdxT"
                     /locus_tag="AC16CB_04290"
     CDS             complement(4752..5348)
                     /gene="pdxT"
                     /locus_tag="AC16CB_04290"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal 5'-phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HIR3341720.1"
                     /translation="MVIGVLALQGDFVEHIAMLRRIGAETREVRLPEQLEDLDGLVIP
                     GGESTTIGKLANDFGMLEPLRRFGEQKAIWGTCAGAIFLSKDVHRQQPLLGLMDIKVE
                     RNAFGRQVASFEVDLDVAALRQIAPNGGPYHAVFIRAPLIESVSGDAQILARLDDGRI
                     VAAQQGRLLATSFHPELTRDDRFHRYFLHLVGTMVERL"
     gene            complement(5342..6229)
                     /gene="pdxS"
                     /locus_tag="AC16CB_04295"
     CDS             complement(5342..6229)
                     /gene="pdxS"
                     /locus_tag="AC16CB_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal 5'-phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HIR3341721.1"
                     /translation="MEQQIATFAVKKGLAQMLKGGVIMDVVTPEHARIAEEAGAVAVM
                     ALERVPADIRADGGVARMSDPELILQIMDSVSIPVMAKCRIGHFVEAQILEAIGVDYI
                     DESEVLTPADEAHHINKHVFKVPFVCGCRNLGEALRRIGEGAAMIRTKGEAGTGDVVE
                     AVRHARSVLGEIRRLQNMPEEELMTFAKEIGAPYELILETRSLGRLPVVNFAAGGVAT
                     PADAALMMQLGVDGVFVGSGIFKSGDPARRAAAIVKAVTHYNDPYILAEVSRNLGEPM
                     VGRQISSIPESELIAGRGW"
     gene            6653..>6744
                     /locus_tag="AC16CB_04300"
     CDS             6653..>6744
                     /locus_tag="AC16CB_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018774649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3341722.1"
                     /translation="MANNKLVLVTGATGYIGGRLAPALIENGYQV"
BASE COUNT         1545 a         1975 c         1784 g         1440 t
ORIGIN      
        1 ttgctgggaa aactggatgc tggtgcggta ataccttgga cggggcgagc caggcgccgc
       61 cgcagcgcat acaacagcgt aaaaatcagc agcgtctcaa caaaccacaa cggtccagaa
      121 ccgatgaagg cttcgtgttt gaggccggga aagtagtccc accacggcag ggtcacgcct
      181 tccgtcacgc gcagatccag atacaccagc agcggactga taatccagct ataaaaggcc
      241 aggggaatcc ccaggcggac cagacggtct ttcaggaatt ttttggagcc tttgcggtcg
      301 taagcccccg gcacaaagta ggccgagatg agcagaaaca ggcccatgaa gtacgcctgg
      361 ctgacgacaa caaaataagc accgatcata ccagttatga aatcttctcc gccttcctta
      421 taaatccatg atcctgtccc ggcgtagatg atcatcagat ggaacagcag cacctggatg
      481 gtcagcatgg tgcggatgtt gtccacgaag agcagccgac cggggggatc cggtcgggct
      541 acggctacag ctttgtccag ccccataagc gcctcgctcg cttcaaagat tttgtggttc
      601 atggttcccc ttgattctct actgcgtaac gaaaacaatc catgccgaag tattgcgtct
      661 tctccctgaa aaccaatccc agcttctcga gcacgcgctg ggaggccata ttttctggat
      721 gcacaatgcc cacaatcatc tccacaccca atcgctcgaa gccatattgc acgctcacct
      781 gtcccccttc cgtggcatat ccttgccccc aaaaggcctt gcccaagata aaatccacct
      841 ctacctcacc tgtatctggg ataacctgca gtccacagcg ccccatgaat tcaccgctct
      901 ggcgacgttc aaccgcccac aggccaaagc cgtgttcagc ccaatgtttc agtatgccgg
      961 agaccatctt ctgcacccgc tccagcgaag gtggatcggt attgggaaaa taacgcaaga
     1021 catccttttc gatcagcatg cgatgaaaag ggacgacatc gtcgtcggta aatgctctaa
     1081 gcgtcaatcg cgaggttgcc agctgcggta ttttcatgtg ggttggcgct ttcctgcgta
     1141 tcggaggaaa ctgcccaatc aatcttgacc tgagctaccc agtcccatgc ctgattcaaa
     1201 ggaaatgtgt atttgggttg gattttctat aatttaacgt ttcagattgt ccggcccata
     1261 ttcagtttac aaaatcttgc aggcaaagtc aataaattgc atcccccgac gtatccctaa
     1321 gtgcatgcta gcgctacagc ttctattttt ctaggcttcc aatcgctcca gaaccaccct
     1381 ttggatatct gcctgcacca ttacgggagg ctgagcggcg tcgattttca cccaacgttg
     1441 cggctcgtcc tgggccaact ggaagtaacc ctgacgcacg cgctggtgga aggccaggtc
     1501 atacgcgtcc aggcgattcc actcgccgct tttggtgcgc cgccgcaagc cttcttctac
     1561 atcaatatcc agcaacaagg tcaggtcggg cttcaagccc ccggtagcga actccacaat
     1621 ggcgcgcagg cgcaccatgt cgacctggtg gccatatccc tggtaagcca gggtcgaatc
     1681 ggcgtagcga tcgcagacca caatacccct tcgggccagt tgtgggcgga taacctgctc
     1741 aacgagctgg gcgcgcgagg cctggaagag caagatttcg gtgcgcggat gcatggctgt
     1801 attctctaga ttggagagca ccgagcgcac ctgatcgcca atggcggtgc ctcctggctc
     1861 gcgcgtggcc agcacgctgg agccgcgctg gcgcaagaag ttgaccagca ggcccgcctg
     1921 ggtggatttt ccgctgccat caggaccttc aagggtaata aacacctatc cctccaaccc
     1981 cctcttcatc ttactaggga aggtaagaag agaacacccc ggaaaatgga gctttgcatg
     2041 gacaatgcgc accccaagct actcgcggaa gatgcgcacc cggcggcgag gctcttttcc
     2101 gtaagaatgc gagagcaggt tagcctggcg agccatctgg tgttgcagtc ggcgcacccc
     2161 agacgaggcc ggtgacagct ccacccagcg ctccccgttc agcacagcgc tgatcgccgc
     2221 catggtctcc tggcggatgc gatcttcacc catctcgtct ggctgttcct cagccaggtt
     2281 gaataagccc atgaggaact gcagcatttg gttgatcgtg ttggcccgca acacatagat
     2341 gggcatgccg cggttctcag catccactac tgttctctgg cgcttgcgat aataagagcg
     2401 cagcgtgacc agcacgtccg cctgcccggg gtcgttaacc agcaccaccg gcacgctcaa
     2461 gcggcgggca gcctgactca agcgattacg cgccacgcca taggcgtaga tgcgcacggt
     2521 ctgtaacggg ccgcggcttg ctctatcttc agcggtgtag gcaggtggtt gagactcagc
     2581 cgggtactca tcgcgaatcg cttggggtga ataagctcgt ccgccattgg tatgctggcg
     2641 gcgcaaacct tgctgttgag aataggccct ggccggctga acaggtttct caatctggat
     2701 gctcccagac tcatctcgcg agcgaatctc tggtgatagt gggaaacctc gcagcagggc
     2761 atccacagag gcggctacat cctcgtgcac cgccaaccgc tggcgggtct ggatctcaat
     2821 gagcacatcg aaggtgggcg gcgagcgccg ttccaaaacc gtcttttggg tgccacgccg
     2881 gcgggcttcc tcgtcggaca aggtgaccga ctcgataccc cctaccaggt cggagagggt
     2941 tgggttcagc agcaggttct ccagagtgtt gccgtgagca gtgccgatta attgcacgcc
     3001 gcgctccgcg atcgtgcgcg cggcagccgc ctctaattcg cgcccgatct catcgataat
     3061 gatcgcctcc gggttgtggt tctccaccgc ttcgatcatc acctcgtgct gcaaggaggg
     3121 tgtgctgacc tgcatgcggc gggcacggcc tacagccggg tgaggcacat cgccatcgcc
     3181 gccgatttcg ttggaggtat ccacaatcac cacgcgcttg ctctccgcca gcaatctagc
     3241 agcctcacgt aacatggtgg tctttcccac gccggggcgt cccaacagaa gcagactctt
     3301 ctcaccctcg atcaagtcct taatgatgtc agtggtgcca tagacagcgc gcccaacacg
     3361 gcaggttagc ccgacaatgt gtccacggcg gttgcgaata gcagaaatgc ggtgcagggt
     3421 acgctccagc ccggcgcggt tatccgcatc gaacgcgcca atgcgctcga cgacatagtc
     3481 gatgtccttg tggggtactt cagtcgggct gagcaccacc tcacgatcca caaaacgcgc
     3541 agtcggcacg cgccccagat ctaggatgac ctccagcagg ttatcgctat cattcgcatc
     3601 tgatactgct tgtgaaatcg attggggaag aacgtcgagt aaagcctgta aatcgtcagt
     3661 tatacgtctt tgcgacataa tatctaatgg ttctccaatt gtgccgccgc ccgtcccccg
     3721 ttttgaggtg atacagcctg ggtttgccta atcggtgcgg tcacctcggg gtggccgcct
     3781 tccaggttcg gcagcgacac ggcgcgtagc gctttctggg ggatggcaag atgtttgacc
     3841 atcatcgcct ggcgctggct gggttctgct cccagctcct caatggtcga ttccagccaa
     3901 gcttggtatg aacgcacgca caaatatacc ggccgggaga gacggtttgg caggtttgac
     3961 agcagttctg ccagcctact ggtcacatct tttagatcgg ggtcgacaaa cggctgcgcc
     4021 cagatacctc tatgcccata cttgagctcc acataagcca gcagttcgtc tccttgacgg
     4081 tagatcaggc cgttgagacg ctcagcgggg aagggttcaa tctgttgcac cagtcccgga
     4141 atgatgttgg catacaaaga gcgcaccggg atgagatccc tatctgaagc atggcgccag
     4201 cctgcagaaa cagacggcat ggtcgggctt cccttcaatt gccagattcg ctggcgcgca
     4261 taaatggcaa aaccggcctg gtgcagcgcc tcaaatgcct gggtattctc atccacgtcg
     4321 gctaacaggc gaaacgcgcc gcgctccacg gcttgtttcg aaaggtgctc caacagggat
     4381 gacaagttgt ccgaatccaa agccccatca ggggtcagga aggtcaactg ggcacattgc
     4441 gatcctaaag aatggaggga ctgcccgatg agcttttggt cttgatcgcc ttcttccacg
     4501 ctcaccgagg tagaaatccc catgtttggc gcgaggctag acaacaatgc actggaaatg
     4561 agcatcggtc cacgcgtcaa aaccagcgag ctatgtagaa acacgctctg ctgccgatag
     4621 cggtgcagtg caggcagatc acgccagtca aacggacgaa tattcattta gatgttagtt
     4681 acaggttgaa cgttgaagag cgtacactga accttcaacc taataacctg caaccattat
     4741 aaaccttcca cctataatct ttcaaccatt gtacccacta aatgtaggaa atagcggtga
     4801 aaacggtcat ctctggttag ctctggatga aaagaggtcg ccagcaagcg cccttgctgc
     4861 gcagcgacaa tacgcccgtc gtccaggcgc gccagaatct gggcatcgcc ggaaacagac
     4921 tcgatgagcg gggcgcggat gaatacggcg tgatatggcc ctccattggg ggctatctgt
     4981 cgcagcgcag ccacatccaa atccacttca aagctggcaa cctgccgtcc aaaggcgttg
     5041 cgctccacct tgatgtccat caagcccaat aaaggttgct gccgatggac atctttggag
     5101 agaaatatcg cccctgcgca ggtaccccag atcgcttttt gctcgccaaa tctgcgcaac
     5161 ggctctagca tgccaaagtc gttcgccagc ttgccaatcg tggtcgattc gccaccaggg
     5221 ataacgagcc catccaggtc ttccagctgc tcaggaagac ggacctctcg cgtttcagcc
     5281 cctatccggc gaagcattgc gatgtgttca acaaaatcgc cctgaagcgc aagaactccg
     5341 atcaccatcc gcgcccagcg atcaattcag actctggaat gctggagatt tgtctgccta
     5401 ccatcggctc acccaggttg cggctaacct cagccaggat atacgggtcg ttatagtgcg
     5461 tcacggcttt gacgatggcg gccgcgcgcc gggctggatc gccagacttg aagatgcccg
     5521 agccgacaaa cacaccgtcc acgcctaatt gcatcatcaa ggcagcatct gcaggtgtag
     5581 ccacacctcc ggcggcaaaa ttcactaccg gaaggcgacc caggctgcga gtctccagta
     5641 tcagctcgta gggagcgccg atctccttgg cgaaggtcat caattcctct tcgggcatat
     5701 tctgcaggcg gcggatttca cccaataccg agcgggcatg gcgcacggct tcaaccacgt
     5761 cgccagtgcc ggcctcgcct ttggtgcgga tcatggctgc accctcgccg atacggcgca
     5821 gagcctctcc cagattgcgg cagccacaaa caaatggcac cttaaaaaca tgcttgttaa
     5881 tgtgatgggc ctcatcggcg ggggtaagca cttccgattc atcaatatag tccacaccga
     5941 tagcttccag gatctgcgcc tctacgaagt gaccgatacg gcatttcgcc attaccggga
     6001 tcgagacgga gtccataatt tgcaagatca attctgggtc gctcatgcga gccacacccc
     6061 catcggcgcg gatgtccgcc gggacgcgct ccagcgccat gaccgccaca gcaccggctt
     6121 cctcggcgat gcgtgcatgc tccggggtaa ccacatccat gatcacgcca cctttaagca
     6181 tctgagctag ccctttttta acggcaaaag ttgcgatttg ctgctccata taccagacct
     6241 cctgagtcga attcgactcg gttttttcaa cattctacca cgcagccata ggcaatgcaa
     6301 tccgccactt ttagagcaac tcgatcgcgc cagttgggtc actggatgga tgaaattaag
     6361 tattccagtc aggtcaagcg gactacccat gtatgccggt gctctcctgg gagcagcaag
     6421 tgtagcacca cgagagggga aaaatagttc ggatcatagg cagtacaatc gccaaagaat
     6481 aattgtatat attttttaca caaatcatct ataatacatt gacaaaaacg tgactttgta
     6541 gtatatttta tatggatttg ggtaaacaat atcgcattta gtctgagatg attatcgctg
     6601 tggctgcaaa gacctgtcct gaaaatagag atctcgcata agaggaaatc aaatggcaaa
     6661 taacaaactc gtactcgtca ctggcgcaac cggctatatc ggcgggcgtt tggcgccagc
     6721 gcttattgaa aatggatacc aggt
//
LOCUS       DBNGSX010000129         6778 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1049383, whole genome shotgun sequence.
ACCESSION   DBNGSX010000129 DBNGSX010000000
VERSION     DBNGSX010000129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6778)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6778)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6778
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1049383"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2390
                     /locus_tag="AC16CB_04305"
     CDS             <1..2390
                     /locus_tag="AC16CB_04305"
                     /inference="COORDINATES: protein motif:HMM:NF014763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3341723.1"
                     /translation="LIFLLGMMTVAMVSVALVAMMINQISGNRAQTISSTALLAQAET
                     YLLQLTKSNARENDKSLELVKRETQKISDYVAAVYENPGAFEQKGIWTVDQHMSFGPN
                     RQYANSASDITSVFVPNFAKIDDNILRDIELGAYLEHLFASAYDNTPNVEAIYFATPH
                     EVTRYYPNIDLGSILPPDFQVTGRVWYKDSILENNPDKDPLWTAPYVDATGLGLVTTS
                     AAPVYSRGGELIGVVGLDLTLSDLIANVEATRLLKSGYTFLIDHTGHTIALPEQGYKD
                     FLGRAPEPGEIYIDLNQSHAQFKPIISRMVVGDTGLEHLNIGNRSIFVAFAPLNSTGW
                     SLGSVIEADDILNSISSLKGQLNDTTRYTVLTRILPITTAIFILIVILGLFFTNRLVV
                     PIQKLAAEAQKIGAGEWDITVPPTTNDEIGVLAGAFKAMAEQIHVFIRDLEKRVAERT
                     RDLERRNTQLQVAAEIAREATAVHELDELLELAVNLIRGRFGFYHAGIFLLDENKEFA
                     VLSAATGEAGREMLARGHKLRVGQTGMVGYASKRGQPRIALNVESDPTHYRNPLLPET
                     RSEMTLPLKVGSVIIGALDVQSHFPDAFSQDDITIMQVLADQLAIAIENARLIQEAQE
                     HVRQLEKLYGTYSKKAWNELRESSITLGYQYDSSGVKPIYRISNGHNTQNVSEAPPPS
                     IQIPIQIRGQAIATLDIWHEGDKLDAETHALLNSIAERLSLALEGARLFEEEHQRAEY
                     ERVVGDVTSQFRATLDIQTVIQTAAREFRKVLNLEEAEVRLTNNQIGEKPDHGTQ"
     gene            2377..5307
                     /locus_tag="AC16CB_04310"
     CDS             2377..5307
                     /locus_tag="AC16CB_04310"
                     /inference="COORDINATES: protein motif:HMM:NF014763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3341724.1"
                     /translation="MEHNNTQIEIVDKPPVNREIEIIDSDQQPESEYAAGLKHYSSAW
                     VLTWTGILASFLAILYFAYLGYRFDAWQLHIVTLSLVGLMLTAVISTVLIRRNKPKGI
                     WFFLVGVQLCFFLYGLLIDRQGLISLGCILAISSLVAIQSLPISGFRRALNLAMIFGI
                     LAALTDFIHLSFQYVMPDSVNYFVATILIVTMIVFCVNLIKNFRSYHTRVQLVFIVTA
                     AGIVALGIFSVVMTAFIERRITQNYNYVLVSTAEHTAKTLDNFFSDNLITIRAESQLP
                     VFSEYLQTPDQKRVNSQLQEDLHITLQGLVQKNPKHVSSYSVLDLRGVNVADTVLKDI
                     GEDRSGLDAFKRVLENGQAYVSPIEFTKNTTIATFNFSAPITDDVGGILGVLMVTYKA
                     DLIQDFLDSYKGSGNENIYVFIVDENHLRLAHTLNPNLRFKKVTPLDQNLWIRLTDER
                     RLPNLPIEELSTNIPSLEKALQVASEYPFYATQFNQPDQGGSQASTMQAGFYSMKTQP
                     WQVVYAVDHAAILHPIQDLMPLAIMLTFILIGLFVAAGLYTADVINKPIKELTETTKL
                     VIAGNWDEEAPVQSGADAGSLAQSFNTLLAQIKLKLEGMENTVSKRDQLSEHHSNQLR
                     ALSEVSQTIVTTRIIDELLPKIVQLISQHFGYSHIGIYLIDATGEYAILQAMISKEGE
                     SISAYAQKIQVAEGGIIGYVINNKEHRIVEDMLEDDLYTNNPDFPDTRSEIAIPLLGG
                     DQVIGVLDIQSREPNAFAQDDITTLLLIADQVAIAIDNARLFRENQGALEAIRRAYSQ
                     LSQDAWAKLLHEQPELGYLANRLDISYTPANGWHPEEVQAAQTGSTIQVSNDTLAIPI
                     KERDHVLGILRLRKPDSHMWSKDELALAETLAQQLYLALENARLYQETQRRAERERLA
                     SEITAKLRASNDPQVIMQTAIQELRRVLNIRSEEVPPNAALRSTDHMPRQQDASSQES
                     QEIG"
     gene            5310..6110
                     /locus_tag="AC16CB_04315"
     CDS             5310..6110
                     /locus_tag="AC16CB_04315"
                     /inference="COORDINATES: protein motif:HMM:NF018290.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR3341725.1"
                     /translation="MTYIIAVANQKGGVAKTTSAVSLGGSLALTGKDVLMIDLDAQAD
                     LTLAMGVTPSKSGGTIADVFFSSANLTGVTRETAIPGMDLIPSFNEMELAERFLPVRP
                     NFEFILRDALKNHLPANFYDYVILDCPPALGAITTNALQAAQMLIIPTQPEFFSAHAL
                     RNMMPIIRRVRNQGNPNMLYRILITMFDKRNRVHRNLSEQLNSTFREGVFQTVIGTDT
                     KLREASIAGLPITHFNIKTRSAGQYQALAQEIIQYVQNPVTKSALLST"
     gene            6268..>6778
                     /locus_tag="AC16CB_04320"
     CDS             6268..>6778
                     /locus_tag="AC16CB_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341726.1"
                     /translation="MGQPVSEFRLTPDSGTLLKSKLEEGAYPIEVAVQYETINGEYIP
                     VVLDILQPPLIEEENGKNRVWHGFARVQLPKITKQERIPRNNEFYPVMPVPKPRSRSQ
                     TTARIKPSSRKTGLAKPSAVEIAPQVAKEEVNNLIELLDASPDRRWSDDELLLIEQVA
                     GQLTLALDNA"
BASE COUNT         1947 a         1636 c         1513 g         1682 t
ORIGIN      
        1 tactcatctt tctattggga atgatgacgg tagcaatggt atccgtcgcg ttggttgcca
       61 tgatgatcaa ccaaatttcg ggaaatcgag ctcaaacaat cagcagtaca gcgctgcttg
      121 cccaagcaga aacctatcta ctgcaactta ccaaaagtaa cgcgcgggaa aatgataaat
      181 ccctggaact cgtcaaaagg gaaacccaaa agatatcgga ttatgttgca gcggtatatg
      241 aaaatccagg tgcattcgag caaaaaggaa tttggactgt agatcaacac atgtcatttg
      301 gcccgaatag gcaatatgcg aattctgcga gcgatatcac cagtgttttt gtaccaaact
      361 tcgccaaaat cgatgacaat attcttcgcg acatcgagct cggtgcctat cttgaacatc
      421 tcttcgcaag cgcctacgac aatacaccca atgtagaggc aatctatttt gctacacccc
      481 atgaagtaac tcgttactac cccaatatcg accttggatc aatattacct cccgattttc
      541 aggtcacagg acgggtttgg tataaagaca gcattctaga aaacaatcca gacaaggatc
      601 cattatggac cgcaccatat gttgatgcaa ctggattagg gttagtcact acttcagcgg
      661 cgccggtata tagtcgtggt ggggaattga ttggggtcgt cggacttgac ctaacattaa
      721 gtgacctgat cgccaatgta gaagcaaccc gcttattaaa aagcggctat accttcttga
      781 ttgatcatac tggccacacc atcgcccttc ccgaacaggg ttacaaagac ttccttgggc
      841 gagctccaga accgggtgaa atctacattg accttaatca gagccacgca caattcaagc
      901 caatcataag cagaatggtt gttggagata ctggtcttga acatctaaac ataggcaacc
      961 gttcaatctt tgtagcattt gcacccttga acagtactgg gtggagcctg ggtagtgtaa
     1021 tcgaggctga tgatatccta aactcaatca gttcattgaa aggccagctc aacgacacca
     1081 ctcgctacac ggtgctgacc cgaatattac cgataactac agcgatattt atcctgatcg
     1141 tcatcctggg attattcttt accaaccggt tggtagttcc catccagaaa ctggcagccg
     1201 aggcgcaaaa aatcggcgct ggtgaatggg atatcaccgt tccacctacc accaatgacg
     1261 agattggcgt tttagccggc gcatttaagg ctatggcaga gcaaatacac gtattcattc
     1321 gcgacttgga gaagcgtgtg gccgaacgca cccgtgacct tgaacgccgc aatacccagc
     1381 tccaggttgc cgcagagatt gcgcgtgaag cgactgctgt ccatgagctg gacgagctct
     1441 tggaattggc tgtcaatctg atccgcgggc gctttggctt ctatcacgca ggcatattcc
     1501 tgctcgacga aaacaaggaa tttgctgtac taagcgccgc cacaggtgaa gcaggcagag
     1561 aaatgctcgc ccgcggtcat aaattgcggg ttggtcagac cggcatggtt ggctatgctt
     1621 ccaaacgcgg gcagccccgt atcgcgctca atgtagaaag cgatccaacc cactatcgca
     1681 atccactact gccggaaact cgctcggaga tgactcttcc actcaaggtt ggaagcgtca
     1741 tcatcggtgc actagatgtt caaagccatt ttcctgacgc tttcagccag gacgacatca
     1801 caatcatgca ggttttagcc gatcaattag ctattgccat cgaaaacgcc cgtttgatcc
     1861 aggaagctca ggaacatgtc aggcaattag agaagttata cggaacatac agcaaaaaag
     1921 cctggaatga actaagagaa tcgagcatta ctttgggtta ccaatacgat tcatcagggg
     1981 tgaaacccat ttatcgaatc tcaaatggac acaataccca gaatgtctct gaagcacccc
     2041 cgccatccat ccagattccc atccaaattc gcggacaagc catagcaacc ctcgatatct
     2101 ggcacgaagg tgataaattg gatgcagaga cacacgcatt attaaactcc atcgcagaac
     2161 gattgagctt ggcgcttgaa ggagcccgat tgtttgaaga ggaacatcaa cgcgccgaat
     2221 atgaacgtgt tgtgggtgat gtcactagtc aatttagagc gactcttgac atccagactg
     2281 ttatacaaac agctgctcgt gagttcagga aagtacttaa tcttgaagaa gctgaggtta
     2341 gattaaccaa caatcaaatt ggtgagaaac cggaccatgg aacacaataa tacccagatt
     2401 gagatagttg ataaacctcc agtaaataga gagatcgaaa tcatcgattc cgatcaacaa
     2461 ccggagtctg agtatgctgc tgggttaaaa cactactcga gtgcctgggt acttacctgg
     2521 acaggtattc tcgcctcatt tctagcaatt ctctacttcg cctaccttgg ttatcgtttc
     2581 gatgcctggc aacttcatat tgtgacactg agcctcgtcg ggctgatgct tacggcagtg
     2641 atcagtacgg tattgatccg cagaaacaaa cctaaaggca tctggttttt tctcgttggc
     2701 gttcaattat gtttcttcct ttacggcctg ttaattgatc ggcaaggctt gatcagtctg
     2761 ggatgcattc ttgcgatttc tagtctggtt gcaatccaat cgcttcccat tagtggattc
     2821 cgccgggctc ttaacctcgc catgattttt ggtattctgg cagcccttac cgactttatt
     2881 cacctatcct tccagtatgt gatgccagat agtgttaatt atttcgttgc cactatatta
     2941 atcgtcacaa tgattgtgtt ttgcgtcaac ctaataaaaa atttccggtc ttaccacaca
     3001 cgcgtccaac ttgtatttat tgttacagcg gccggtatag tcgcattggg aatattttct
     3061 gttgtgatga ccgcttttat cgaacgtaga atcacacaaa actacaatta tgtcttggtc
     3121 agcacagcag agcataccgc aaaaacgctg gataatttct tttcagataa cctgattaca
     3181 attcgtgccg aaagccaact cccagtattt tcagaatatc tacaaacacc tgatcaaaaa
     3241 cgcgtgaaca gccaattgca ggaagaccta catattacat tacaaggttt ggtgcaaaaa
     3301 aatcccaaac atgtctcttc atacagcgtt ttagatctca ggggagttaa tgtagcggat
     3361 acggttttga aggatatcgg cgaggataga tcagggcttg atgcattcaa acgcgttttg
     3421 gaaaacggtc aagcatatgt atctcccatc gagttcacaa aaaacactac cattgccacg
     3481 tttaatttca gcgcaccgat caccgacgat gtagggggga tactaggtgt ccttatggtg
     3541 acctacaagg cagaccttat acaagatttc ttggattcat ataaaggctc aggcaacgaa
     3601 aacatatatg tgtttattgt agatgaaaat catctcagac tggcgcatac gctgaaccca
     3661 aatttgcgtt ttaaaaaagt aacaccgcta gatcaaaatt tgtggatacg cctgaccgac
     3721 gaaaggaggt tgccaaattt acccatcgaa gaactttcaa cgaacatacc gtcattggaa
     3781 aaagccctgc aggtagcatc ggagtatcca ttctatgcca cccaattcaa tcaacctgat
     3841 caagggggga gtcaggcaag tacaatgcaa gcagggtttt actctatgaa aacgcaacct
     3901 tggcaagtgg tatatgcggt cgatcacgcc gcgattctcc atccaataca ggatttaatg
     3961 cctcttgcta tcatgttgac tttcatactt atcgggttat ttgtcgcggc tggcttgtac
     4021 acggctgatg tcatcaataa accaatcaag gaactgactg aaactacaaa gcttgtcatt
     4081 gcggggaatt gggatgagga ggcaccagtc caaagtggtg ctgatgccgg ctcgctggct
     4141 caatcgttta acaccctgct ggctcaaatt aaattaaaac tcgagggtat ggaaaatacg
     4201 gtttcaaaac gcgatcagtt atcggaacat cattccaatc aactgcgcgc tttgtcagag
     4261 gtaagccaaa ccatcgtcac tacccgcatt attgacgagc tcctgccaaa gattgtacaa
     4321 ctaatcagcc agcacttcgg ctactcacac atcggcatat acctgattga cgcaaccggt
     4381 gaatacgcaa tacttcaagc aatgatcagc aaagaagggg agagcataag cgcttacgcc
     4441 caaaaaatcc aagttgccga aggaggaatc atcgggtatg taattaataa taaggaacat
     4501 cgtatcgtag aagacatgct tgaagatgac ctatatacta ataaccccga ttttccagac
     4561 acgcgttctg aaatagccat acctttactt ggtggtgacc aagtaatcgg ggtgctggat
     4621 attcaaagca gagagccaaa tgcatttgcc caagacgata tcaccacatt gctccttatt
     4681 gccgaccagg ttgctattgc tatcgacaat gcccgcctgt tccgcgagaa tcaaggggct
     4741 ctcgaagcca ttcggcgcgc ttacagtcaa ctaagccaag atgcatgggc aaagcttctt
     4801 catgagcaac ccgaattggg ctatttggct aaccgcttgg atattagcta tacaccggcg
     4861 aatggttggc accctgaaga ggtccaagcc gctcaaaccg gtagcacaat acaagtcagc
     4921 aacgatacac tcgccatccc cataaaggaa cgagaccatg ttttgggaat attgcgcttg
     4981 cgcaagcccg actcccatat gtggtctaaa gatgagctcg cattggcaga gacactcgcc
     5041 caacagcttt acctggcttt ggaaaacgcc cgtctgtatc aagaaactca aaggcgcgcc
     5101 gaacgcgaac gcctcgctag cgaaataacc gcaaaattgc gcgccagcaa tgatccccaa
     5161 gtgataatgc agaccgcaat tcaagaattg cgacgggtat tgaatatccg tagtgaagaa
     5221 gtccctccca atgcagctct acgatcaaca gatcacatgc cacgtcaaca agacgcttca
     5281 agccaggaaa gccaagagat tggatgaata tgacctacat aatagctgtt gctaatcaaa
     5341 aaggcggtgt tgccaagacc acctcagcgg tgtccctggg tggttcattg gctctcactg
     5401 gaaaagatgt attgatgatt gacctcgatg cccaggctga tttgacttta gcaatggggg
     5461 ttaccccttc aaaatctggt ggcacaatcg cagatgtgtt tttcagctcg gctaacctga
     5521 caggggttac gagagagaca gcaattccag gcatggattt aattccatca tttaatgaaa
     5581 tggagttggc tgagcgattt ttacctgtcc ggccgaattt cgagttcatt ttacgagacg
     5641 cgttgaaaaa tcacctgccc gcaaattttt atgattatgt cattcttgat tgtcccccag
     5701 cactgggtgc catcacaacc aatgcgcttc aagccgccca aatgcttatc attcccacac
     5761 agccagagtt tttctctgca catgcattgc gcaacatgat gcccattatc cggcgagtcc
     5821 gcaaccaggg caacccgaac atgctctacc ggatattgat taccatgttt gacaaacgga
     5881 atcgcgtgca tcgtaatttg agtgaacaac tgaactcaac ctttcgcgaa ggtgtattcc
     5941 aaaccgtaat tggtacagac actaaattgc gagaggcctc tatcgcgggc ttacctatca
     6001 cgcatttcaa tataaaaact cgaagtgctg ggcagtatca agctctggct caggagatta
     6061 ttcagtatgt ccagaatcca gttacaaaat ctgcactcct ctcgacttaa ttcacttttc
     6121 gccgacctgg gtaaagaaag ccatgatatc ccggtaacca gcaataagac tgtttcgggg
     6181 tggacctggg aatgcgattc tcaaggctac ttcacagatt gcagccccga ggttgagtca
     6241 attctaggta tccctgttaa ggaatttttg ggtcaaccgg tttctgaatt ccgtttgaca
     6301 cccgattcag gcacactcct aaaatccaag ctggaagaag gcgcttatcc catcgaggtt
     6361 gctgtccaat acgaaacgat aaatggagaa tacataccag tcgtattgga catactccaa
     6421 cctcccttga ttgaagaaga aaacggcaag aatcgagtct ggcatggatt tgccagggtg
     6481 cagttaccca aaataaccaa acaagaacga attccgcgca acaacgagtt ttatcctgta
     6541 atgccggtcc ccaagccgag atccagatca caaacaacgg cgcggatcaa accaagtagc
     6601 aggaaaaccg gtttggcgaa gccatctgct gtggagatag cccctcaagt tgcgaaagag
     6661 gaagttaata atttaattga gcttctagac gcgagccctg atcgtcgctg gagtgacgat
     6721 gaacttttat tgattgagca ggtggctggt caattgacgc ttgctttgga taatgcca
//
LOCUS       DBNGSX010000130         3605 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1741299, whole genome shotgun sequence.
ACCESSION   DBNGSX010000130 DBNGSX010000000
VERSION     DBNGSX010000130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1741299"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="AC16CB_04325"
     CDS             complement(<1..412)
                     /locus_tag="AC16CB_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR3341727.1"
                     /translation="MLGYEAVIGLEVHAELETRSKMFCSCARVDSTTAQPNSAVCPVC
                     AGMPGVLPVVNRRAVEYALRVALALECEIAHTSLFVRKNYFYPDLPKGYQISQYEQPL
                     ARNGRLTILTSQGERVINVRRVHLEEDTGKLTHVQ"
     gene            complement(570..1763)
                     /locus_tag="AC16CB_04330"
     CDS             complement(570..1763)
                     /locus_tag="AC16CB_04330"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR3341728.1"
                     /translation="MTTIAPSKGADLGKKYLATRVANLKPSGIRKFFDIVATMQDVIS
                     LGIGEPDFDTPKPILEAGLRSLQDGHTHYTSNAGQLELRQALCEHLYSLYEVKYDPAD
                     LVITVGVSEALYLAMTALLNPGDEVIVPTPCFVAYQAEVILAGGVAVELPTAAQDGFQ
                     LRPDALKAAITPRTKAILLGYPNNPTGAVASRETLLEIARIAEEHDLVVVSDELYDQL
                     VYGVSHVCFAALPGMQDRTILLGGFSKNYAMTGWRVGFAAGPADIIKGLLRVHQYTIM
                     SAPTTAQEAALEAIKNGQPHVEEMRREYDRRRKLIVTGLNELGLATVEPHGAFYAFPS
                     IAMTGMDEETFARKLLEEESVAVVPGTAFGPGGEGYVRCSYATAYEKIEEALHRIERF
                     TRRHG"
     gene            complement(1775..1987)
                     /locus_tag="AC16CB_04335"
     CDS             complement(1775..1987)
                     /locus_tag="AC16CB_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341729.1"
                     /translation="MVDLVECHSEVTYAERPTALIWQGQRLPVDEVLARWRVPGAVRF
                     RVRTEGGLVFELEYDEVKDIWQVEER"
     gene            complement(2029..3039)
                     /locus_tag="AC16CB_04340"
     CDS             complement(2029..3039)
                     /locus_tag="AC16CB_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR3341730.1"
                     /translation="MDQTLKIVIPMAGLGTRLRPHTWSKPKQLIGVAGKPVLDHVLDT
                     LSTLPDPENIELINIVGYRGEQIEAHLGARYPHLKAHYVLQEDPRGQSHAIYLAKEYL
                     KGPMLVIFADTLIETDLSFLASEPAEAVVWVKPVPDPRRFGVAVVGDDGWVDRLIEKP
                     QEMTNNLAVVGFYYIRSAEDLVSAIEEQMKRNMQLKGEFFLADAINIMLERGLRMRTQ
                     KVDIWLDAGTPEALLETNRYLLENGRNNSAQLADREGLVVIPPVFIHPTAEVIHSIVG
                     PNVSLGAGCRVERSILSDSILEDEAQVLDVILEKSLVGRRAHIQRRAGVISAGDQTVV
                     IL"
     gene            3145..>3605
                     /locus_tag="AC16CB_04345"
     CDS             3145..>3605
                     /locus_tag="AC16CB_04345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HIR3341731.1"
                     /translation="MENDTLRQAFRLLNKFIMVPLFRLGLGALVGNPLTGYIMVIQTV
                     GRKTGRLRYTPVNYALMDGYVYCMSGWGAKADWHRNLMTNPRLEVILPGGNLSGAAEV
                     VAEPGEWLRAARQLLKNAGLAGFLSGVNPFTASDEQIRQKYQGYPIMRIRPT"
BASE COUNT          769 a         1010 c         1027 g          799 t
ORIGIN      
        1 gctgcacgtg ggtgagcttg ccggtgtctt cttccaggtg aacgcggcga acattgatga
       61 cgcgctcccc ttgcgaggtc aggatcgtca accgaccgtt tcgagccagg ggttgctcgt
      121 attgggaaat ctggtagcct ttcggcaaat ctgggtaaaa gtaattcttg cggacgaaaa
      181 ggctggtatg ggcgatttca cactccagcg ccagcgctac gcgcagggca tactccacgg
      241 cgcggcgatt tacaaccggc aatacaccgg gcataccagc gcagacgggg cacacagcgg
      301 agttgggctg tgcggtggtt gaatcgaccc gcgcgcaaga gcagaacatc ttggagcgcg
      361 tttctagctc ggcgtgcact tccagcccga tgacggcttc gtaacccaac atgcttatcc
      421 gcaggtcata ctgaactaga atccggggtt atgggaactt gcgtggcaca ggggaagtat
      481 ggtgagtgcg ggcgggtcat gacctgctca cacaccgcac atccggctct gaccacggca
      541 tatatcaatg agcccgggag ttttcatctc tatccatggc ggcgcgtgaa gcgctcgata
      601 cggtgcaagg cctcttcgat cttctcgtaa gctgtggcat aggaacagcg cacatacccc
      661 tccccgcctg gtccaaaggc tgtgccaggg acgacagcca cgctctcctc ttccagtaat
      721 ttgcgggcga aggtctcctc gtccatcccg gtcatagcga tactcggaaa ggcgtagaat
      781 gcgccgtgtg gctcgacagt cgccagcccc aattcattca agcctgtgac gatcagctta
      841 cgccgccggt cgtactcgcg gcgcatctct tccacatgtg gctgaccatt ctttatggct
      901 tccagtgcag cctcctgagc agtggtgggc gcagacatga tcgtgtactg atgcacgcgc
      961 aagagacctt tgatgatatc cgccggtcca gcggcgaaac cgacgcgcca gccagtcatg
     1021 gcatagtttt tggagaaacc gcctagcaat atcgtgcggt cctgcatgcc gggcagggca
     1081 gcaaagcaga cgtgagacac accatagaca agctggtcat acaattcgtc agataccaca
     1141 accaggtcat gttcctcggc gatgcgggct atttcaagca atgtctccct actagcgacc
     1201 gcgccggtgg gattgttggg gtagccaagc aagatggcct tcgtgcgggg cgtgatggct
     1261 gccttcaggg catcggggcg caactggaag ccatcttgtg ctgctgtggg aagctccaca
     1321 gccacgccgc ctgccaggat cacctctgcc tggtaagcga caaagcatgg ggttggtacg
     1381 atgacttcgt cgccaggatt gagcagggca gtcattgcca gatagagcgc ctccgagacg
     1441 cccaccgtga tgaccagatc ggccgggtca tatttcacct catacaagct gtacaggtgt
     1501 tcacacagcg cctggcgtag ctccaattgc ccggcattgg aggtgtagtg cgtgtgcccg
     1561 tcctgcaggg agcgcaagcc agcttctaag attggcttag gtgtgtcaaa atccggttca
     1621 ccaatgccca gcgagatcac atcttgcatg gtggcgacaa tgtcaaaaaa tttgcggatg
     1681 ccggagggtt ttaagtttgc gacgcgggtg gcgaggtatt ttttgcccaa atcggctcct
     1741 ttagatggcg caatggtggt cattagactt catcctatcg ttcctcaact tgccagatat
     1801 ctttgacttc atcatattcc agctcgaaga ccagaccgcc ctcggtgcgg acgcggaaac
     1861 gcactgcccc gggaactcgc cagcgtgcca gcacttcgtc cacgggtagg cgttgccctt
     1921 gccagatcag ggcggtcggt cgctcggcgt aagtcacttc agaatgacat tcaaccaaat
     1981 ctaccatatt aagggcttgc gtacttcccg aaggtgtaac ttgtgtaatt ataagatgac
     2041 cacggtctgg tcgccagcac tgatcacgcc agcgcggcgc tggatgtgcg cacggcgacc
     2101 caccagagat ttttccagga taacatccaa cacttgggct tcgtcttcca ggatcgaatc
     2161 tgagaggatg ctgcgttcca cgcggcaacc tgcacctaat gaaacatttg gtccgacgat
     2221 ggagtggatc acctcggcag tgggatggat gaacactggc gggatgacca ccaagccctc
     2281 acggtcggca agttgggcgg aattgttgcg cccattctcc agcagatagc ggttggtctc
     2341 cagcagcgcc tctggcgtcc cggcatctaa ccagatatcc accttctgtg tgcgcattct
     2401 caagccgcgc tctagcataa tgttgatggc atcggccagg aagaactcgc ctttgagctg
     2461 catgttgcgc ttcatctgct cttcgatggc ggacaccaag tcctcggcgg agcggatgta
     2521 ataaaaaccc accacggcca ggttgttggt catttcttgc ggcttctcga tcaagcggtc
     2581 gacccaacca tcgtcgccca ccacagccac gccaaagcgg cgcgggtcgg gcacgggttt
     2641 tacccatacc accgcctccg ctggctcgct ggccaggaaa gatagatcgg tctcaatcag
     2701 ggtgtcggca aagatcacca gcatgggacc tttgaggtat tccttcgcca aatatatggc
     2761 gtgcgactgt ccgcgcgggt cttcttgcag cacgtaatgt gccttcaagt ggggatagcg
     2821 cgccccgaga tgagcctcaa tttgctcgcc gcgatagcca acgatgttga tcagctcgat
     2881 attttctggg tcggggaggg tggataaggt gtccagcaca tgatccagga cgggcttgcc
     2941 cgccacgccg atcaattgct tgggtttgct ccaggtatgc gggcgcaggc gggtgcccag
     3001 cccagccatg gggatgacga tctttagtgt ttgatccata aaaatgccgt ttatcctata
     3061 ataagtataa agttctgagt aatttcgttt ctaccatcat tacaatttca aacaagttgc
     3121 gttgcgcaaa tattataacc tgatatggaa aacgatacct tacgccaagc ctttcgcttg
     3181 cttaacaaat ttattatggt acccctcttc cgtttggggc taggggcgct ggttggcaat
     3241 ccgcttaccg ggtacatcat ggtcatccag acggtaggcc gcaagacggg gcgcctccgc
     3301 tacaccccgg ttaactacgc cttgatggac ggctacgttt attgcatgtc aggatggggg
     3361 gcgaaggccg actggcatcg taatttgatg accaacccgc gcctggaggt gattttacca
     3421 gggggtaacc tgagcggcgc ggctgaagta gtggctgaac ctggcgaatg gctgcgagct
     3481 gcccgacagc ttttgaagaa cgctggcttg gctggttttt tatcgggcgt gaaccccttc
     3541 accgccagcg acgagcagat ccgccagaaa taccaggggt acccaatcat gcgcatccgc
     3601 ccgac
//
LOCUS       DBNGSX010000131         5091 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1672704, whole genome shotgun sequence.
ACCESSION   DBNGSX010000131 DBNGSX010000000
VERSION     DBNGSX010000131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1672704"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..1370)
                     /gene="nagA"
                     /locus_tag="AC16CB_04350"
     CDS             complement(231..1370)
                     /gene="nagA"
                     /locus_tag="AC16CB_04350"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HIR3341732.1"
                     /translation="MRNSELTLTGLALLPDGVISEATIHISNGTITQVSRGADPQADI
                     QTTGTILPGFIDLQLNGAFGYDFTLNGASVAAVSERLPSTGVTSFIPTIITSPFKDYP
                     VRFSEIAGAMQIAKGAQPLGVHLEGPYLNPAKRGAHDAAYMRPIDLDEILGWADPALV
                     RIVTLAPELPGALEAIRALRNNGITVSLGHSNATLEQANAGFEAGANWATHLYNAMSP
                     PHHREPGLTGAILTSSVPCGLIPDGVHVHPSMVNLAFHAKGVNGITLVTDAMEALGMP
                     SGIYRLGDREVHVSEDRVQLADGTLAGSKIEMDAAVRNMMAFSGCTLAQAASMASLIP
                     AQVLGLDRKGAIGPGYDADLVILDQTLHVSLTIVAGKLSFERSPV"
     gene            1491..2825
                     /locus_tag="AC16CB_04355"
     CDS             1491..2825
                     /locus_tag="AC16CB_04355"
                     /inference="COORDINATES: protein motif:HMM:NF015948.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR3341733.1"
                     /translation="MARNLDHKPVFLTMALLPMVMEKVFLFKAEGYDPRWIEAGIKQA
                     AQALLVSLPAQGDVFLHVDCPWADSRFAPHAHTHPAMIEGVAKALSGASLSLGGCSLP
                     YFPTRYSFKQAGYDALARKLDAELVAFEEVATRQVELGGTAVVNKQVGLPSAWDQAAF
                     RISLPKLRCSTLVSFAGALRHMQSLLSQAAQVTDSHRLPEKMVDLLPTVSPNLIVVDA
                     IHALHKGGELSGEPVELGLIIIGTHPLAVDVVCAVLLGLEPPRVDFLGEAASRGLGPS
                     SLDEIELLGDLTLDEVRSLSQKVQMVDPDPVHYVLPKQVKILRSEKARQAGSSGNLTD
                     VLSLIERSGVSLKSATEATIVIGAVAQIPPPTSDTATLIFIDDTSRGEYTGYSRIVRL
                     PGRNIPISQMLNDVPYALKVINLRAELGAGYLTAKLGSNLTRIVRRMLGGKV"
     gene            2956..4050
                     /locus_tag="AC16CB_04360"
     CDS             2956..4050
                     /locus_tag="AC16CB_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIR3341734.1"
                     /translation="MFATLQRIGRSLMLPIAVLPAAGILLRFGQADLLNIPWLADAGG
                     AIFGNLALLFAIGVAVGFTADVGTAGIAGAVGYWVFTKVLVDISNSIYQAEVNMGVLA
                     GILMGLTAAWLYERYHTIQLPEWLAFFGGKRFVPIVTAFVALVLGIVFGFIWHWPEQW
                     LAAVGNWATGAGPMGAAVHAFLNRLLIPLGLHHVINSIVWFQFGDLNNFFDTAGQTGG
                     LFMTGFFPIMMFGLPAAALAMYVTAKDANRRAIGGVLFSAALTSLITGITEPVEFAFM
                     FVAPVLYVVHALLTGVSAFVTIALGLRDGFTFSAGLIDYILNFGIATNPIMLLVVGVI
                     FGVIYYFVFVTLIKALNIPTPGREPEVVEE"
     gene            4229..4474
                     /locus_tag="AC16CB_04365"
     CDS             4229..4474
                     /locus_tag="AC16CB_04365"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIR3341735.1"
                     /translation="MEEKARQFIEALGGKENIVEIEGCITRLRGTLRDPSKVDMKKLQ
                     SLGVIGRPILMGKGIQVVVGTHAELIGTAMNKIMRAS"
     gene            4471..4938
                     /locus_tag="AC16CB_04370"
     CDS             4471..4938
                     /locus_tag="AC16CB_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR3341736.1"
                     /translation="MRIVPVYSPFTGRVIPLEDVPDPVFSAKMLGDGLAVLPETDQVV
                     APISGKLTVLHSAGHAFAVQAHEAPVTVLVHIGLDTVKLKGVGFKILTSLGDQVTLGQ
                     PVVQVDREAIEHAGYSLLSPVILPDLPEGIKVELTDTAKVVQGVDVLLNVVVP"
     gene            4953..>5091
                     /locus_tag="AC16CB_04375"
     CDS             4953..>5091
                     /locus_tag="AC16CB_04375"
                     /inference="COORDINATES: protein motif:HMM:NF051685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7916 family protein"
                     /protein_id="HIR3341737.1"
                     /translation="MTSVKRLLDANASDFAAMTAPQLIESIRQAEGRTVAAEVIATAP
                     PL"
BASE COUNT         1095 a         1273 c         1419 g         1304 t
ORIGIN      
        1 gtgcagcgaa agagatagcc acgataattt ctagaatata aaccaggagg ctggtggtca
       61 ggcttctaac caattgccct gggcgaagtt catccgccat ccaggtagtt agacgcgctt
      121 gcagggtcag ctttgattca ggtgatgcca tctttccacc tctaatctcg attgaaagcg
      181 atatgtaaca tgtaaagagg tcgctttact gtaaggaact cttaatccaa ttaaacgggc
      241 gacctttcga aagacagctt tcctgccaca atggttaagg aaacatgcag tgtctggtct
      301 agtatgacca gatcggcgtc atagccaggc ccaattgcgc ctttacgatc taatcccagc
      361 acctgcgctg gtataaggga cgccatgcta gctgcctgcg ccagtgtgca tccagagaaa
      421 gccatcatgt tgcgcaccgc ggcgtccatc tctattttgc ttccagccag tgtgccgtcg
      481 gctaactgca cgcgatcctc actgacatgg acctctcgat ctcccaggcg atagatgcca
      541 gaaggcatgc ccaaggcttc catggcatcc gtgaccagcg ttatgccatt tacccctttg
      601 gcgtggaatg ccagattgac catgctcggg tgcacgtgca cgccatccgg aatcagccca
      661 cacggcactg atgaagtcag tattgctccg gttaatcctg gctctcgatg atgagggggg
      721 ctcatggcat tgtacaagtg cgtggcccaa ttcgctcccg cctcgaaccc ggcgttggct
      781 tgctcaagag tggcattgga atgtccaagg ctaacagtga tcccgttgtt gcgcagggcg
      841 cgaatggctt ccagcgcgcc gggtaattct ggggcaaggg tgacgatgcg aacaagcgcc
      901 ggatcggccc agcctaggat ctcgtctaaa tcgatggggc gcatatatgc tgcatcatgt
      961 gcaccgcgct tggctgggtt aagatagggg ccttccagat gtacgcccag cggctgagcg
     1021 cctttggcga tttgcatcgc ccctgcgatc tcgctgaacc gcaccgggta gtccttaaat
     1081 ggtgacgtga ttatggtagg aataaagctt gtcacgccag tggacggcaa tcgctccgac
     1141 acagctgcca cgctggcgcc attcagggtg aagtcataac cgaaagcgcc gttgagctgc
     1201 aggtcgatga agcccggcag gatggtccct gtggtctgta tgtctgcctg tggatctgca
     1261 ccgcgagaaa cctgggtgat ggtgccattg ctaatgtgga tagtagcttc ggagatgacg
     1321 ccgtcaggta gcaatgctag tccggttaga gtcagttcag aatttctcat aggattcaat
     1381 ttatggaatt tacttgattt ggactcaaat attatttatg ctctatggaa gcttaatatt
     1441 atcacatgga attaagcaat tgaagccttt attcccagta ttttcgtttt atggcgcgta
     1501 acctagatca taaaccagtg tttttaacta tggcgttgct acccatggta atggaaaaag
     1561 tatttctctt caaggccgaa gggtatgatc ctagatggat tgaggcaggg atcaagcaag
     1621 cagctcaagc cttgctagtt tctctgccag cgcaagggga cgtgtttttg cacgtcgatt
     1681 gcccttgggc tgattcgcgt ttcgcacctc atgcgcacac gcacccagct atgatcgaag
     1741 gcgttgcgaa agcactttca ggcgcatcgc taagcttggg cggttgcagt ttgccgtatt
     1801 ttcctacacg ctatagtttc aagcaagccg gctatgatgc tcttgcgcgt aagctcgatg
     1861 cggagctggt tgcatttgag gaggtggcga cgcgtcaggt cgaactgggt ggcaccgctg
     1921 ttgtgaataa gcaggtgggc ttgccttctg cctgggatca ggcagctttc cgaatctccc
     1981 tgcccaagct gaggtgcagc acgctggtct cttttgccgg tgctctacgg catatgcaga
     2041 gcttgctgtc acaagctgcc caggtcacgg acagccatcg cctaccggag aagatggtag
     2101 atctcctgcc cacggtgtcc cctaatctca tcgtagtgga tgctatccat gcgcttcata
     2161 agggtggtga actctccggt gagccagtgg agctggggtt gatcatcatc ggcacacatc
     2221 cacttgcggt agatgtggtc tgcgctgtgc tcctgggcct cgagccaccc cgggtcgatt
     2281 tcttgggtga ggctgcctca cgcggtcttg gtccttccag cctagatgag atcgaattgc
     2341 tgggtgatct gacccttgat gaagtgcgtt cactgtccca gaaagtgcaa atggttgacc
     2401 ctgatccagt gcactatgta ttgcccaagc aggtgaagat tctgcgctca gaaaaagccc
     2461 gccaggcggg tagttcaggc aacctgaccg atgtgttgtc cttgatagag cgctccggtg
     2521 taagcttgaa atcagcgacg gaggctacca tcgtcatcgg tgcagtggcg caaataccgc
     2581 cgcctacatc agatacggct acactgatct tcatagacga tacctcgcgc ggcgagtaca
     2641 ccggctacag ccgcatagtt cgattaccgg ggcgaaatat cccgattagc cagatgttga
     2701 acgatgtccc ttatgctctg aaagtgatca acctgcgcgc tgaattaggt gcaggctatc
     2761 tgacggcaaa attgggctct aatctcacac gtatcgtgcg acgaatgttg ggtgggaaag
     2821 tctgataatc aactcgcaca atgaggaggt gaatcgcgaa caaaaaattc gatgggctcg
     2881 agtacgggtg acaaattttc accagagcct ctcatcataa ttctatccag attatcattt
     2941 agttaggagg attcaatgtt tgctacacta caacgtattg gacgttcgct catgctcccg
     3001 atcgccgttc tgcctgcggc gggtatcctg ctccgctttg gacaggctga cctgcttaac
     3061 ataccctggc tagcagatgc gggtggcgcg atctttggga acctggcact tctatttgcc
     3121 attggcgtgg ctgtaggctt cactgctgat gtcggcacag ctggtatagc cggcgctgtg
     3181 ggttactggg tgtttaccaa agtcctggtt gatatatcga atagtatcta ccaggctgag
     3241 gtgaacatgg gagttctggc tggcattcta atgggattga ccgctgcttg gttatatgag
     3301 cgttaccata ccatccagct gcccgaatgg ctggctttct ttggtggcaa gcgctttgtc
     3361 ccaatagtga cagcctttgt ggcattggta ttggggatcg tatttggctt catctggcac
     3421 tggcctgagc aatggctggc ggcggtaggg aattgggcta ctggcgcagg ccccatgggc
     3481 gcagcggtgc atgccttctt gaaccgcttg ttgatcccac tcggactgca ccatgtgatc
     3541 aattccatcg tctggttcca gtttggcgat ctgaacaact tctttgatac cgctggccaa
     3601 accggtggcc tgttcatgac gggcttcttc cccatcatga tgttcggact gccggcagca
     3661 gccctggcga tgtatgtcac tgccaaagat gcaaatagac gagctatcgg gggtgtgctc
     3721 ttcagcgccg ctttgacctc gctgataacg ggcatcaccg agccggttga atttgctttc
     3781 atgttcgtcg ctccggtatt gtacgtcgtg cacgccctgc ttaccggcgt ctcggcattt
     3841 gtgaccattg cacttggcct gcgagacggc tttacttttt cggctggctt gatcgactac
     3901 atcctcaact ttggcatcgc taccaacccc attatgctgc tggtagttgg cgtgatcttt
     3961 ggcgtgatct attacttcgt cttcgtgacg ttgatcaaag ccttgaacat ccccacgcca
     4021 ggtcgcgagc ctgaagtcgt cgaggagtaa ttctgcaaga agtttgtacc ctttgaaatg
     4081 ggctgtccaa aatgtgcggt ttgataaacc gccgcctgtt gcagccggag tgattatccg
     4141 gcctacagct agagtttatg atggatcttt tttctttttg gacagcccaa tttccccttt
     4201 gttggcaatt gaggaggtta tttagattat ggaagaaaag gcccggcagt ttattgaagc
     4261 cctgggaggc aaagagaaca ttgtcgagat tgaaggttgc atcacccgct tgcgcggtac
     4321 gttgagagac ccttcaaaag tggacatgaa gaaactgcaa tcgctgggtg tgattggtcg
     4381 gccgatcctg atgggcaaag gtattcaggt ggtggtcgga acacacgctg agttaatcgg
     4441 cacagccatg aacaaaatca tgcgggcttc atgaggatcg ttccggtcta ttcccctttc
     4501 accggcaggg taatacctct agaggatgtt ccagacccgg tattctcggc taaaatgctt
     4561 ggtgatggat tggcggtcct gcctgagaca gatcaggtgg tggcgccgat cagtggaaag
     4621 ctgactgtgc tgcatagcgc cgggcacgca tttgctgtcc aggcgcatga agccccggta
     4681 actgttttag tccatattgg gctggacacc gtgaagttga agggcgtcgg gttcaagatc
     4741 ttgaccagtc ttggcgatca ggtcacgctt ggacagccag tggttcaggt tgatcgagag
     4801 gcgatcgaac atgctggata ttctcttctt tcaccagtaa tattgccaga tttacccgag
     4861 gggataaagg tcgagcttac ggatactgca aaagtggtcc agggggtgga tgtgctgctc
     4921 aatgtagttg ttccataagg atccaaatcg tgatgacgtc agttaaacgc ttattagacg
     4981 ccaatgcgtc agattttgcc gcaatgactg cgcctcagct tattgagagc attcgccagg
     5041 cagaggggcg cacagtggct gctgaggtga ttgccaccgc cccgccgttg c
//
LOCUS       DBNGSX010000132         5721 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1187861, whole genome shotgun sequence.
ACCESSION   DBNGSX010000132 DBNGSX010000000
VERSION     DBNGSX010000132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5721)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1187861"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="AC16CB_04380"
     CDS             complement(<1..290)
                     /locus_tag="AC16CB_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341738.1"
                     /translation="MSLKSTIRRISSSRWTAEAIAVLAGIAYLVQSVIYAHSQESILD
                     EGAYLYKGYLFVTGQYWPYQDYGPWSNHMPLSFLIPGAVQFLIGPDLRTARY"
     gene            complement(287..1255)
                     /gene="gmd"
                     /locus_tag="AC16CB_04385"
     CDS             complement(287..1255)
                     /gene="gmd"
                     /locus_tag="AC16CB_04385"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR3341739.1"
                     /translation="MPVALITGITGQDGSYLADFLLAKGYKVVGMVRRSSTVTFERIQ
                     HIQDDITVIQGDLHDQSSLVDVMEEYQPDEVYNLAAQSFVPTSWNQPVLTGEVTALGV
                     TRILEAIRHSNPKARFYQASSSEMFGKVREVPQRETTPFYPRSPYGVAKVYGHWITVN
                     YRESYDLYAVSGILFNHESPRRGLEFVTRKITYNAARIKLGLTKELRLGNLEARRDWG
                     FAGDYVEGMWLMLQQDEPADYVLGTGVTHSVREFCEAAFSYLGLDYNDYVIQDPRFYR
                     PAEVDLLVADPGNAKEKLGWQPRVSFQELIQLMVNADLRRVSAGIS"
     gene            1365..2063
                     /locus_tag="AC16CB_04390"
     CDS             1365..2063
                     /locus_tag="AC16CB_04390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HIR3341740.1"
                     /translation="MAYLTGGERARYVQGMFAHIARRYDLMNRLMTAGQDVRWRREVI
                     RRAELPASGRLLDLGAGTGDLAREALRQVPKSLPVAADFTLEMMRVGKTRPNMGLEWA
                     AADALCLPFPDGSFDAVVSGFLLRNVSDILQSLNEQRRVLKPGGRIIALDTTQPPKNP
                     LAPLIKLHLHTVIPTLGRLIAGSAEAYRYLPETTENFLSAEGLAERMQTAGFQGVGFR
                     RLMFGTVAIHWGFK"
     gene            2080..2277
                     /locus_tag="AC16CB_04395"
     CDS             2080..2277
                     /locus_tag="AC16CB_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341741.1"
                     /translation="MTKSLLTMSLPNASSSSEFHTARFPFVSKFRGEIERNALYIFIS
                     RWTRLMGNRSGFNFNQGWYLP"
     gene            2274..3026
                     /locus_tag="AC16CB_04400"
     CDS             2274..3026
                     /locus_tag="AC16CB_04400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3341742.1"
                     /translation="MSAASRDFEKAALRGEPSYVWRAGQERRLKMILAAAGERVKGRV
                     LENGCGVGMYAAHLLPHAGSVTGLEYDFERAGQASKVPGQVVNAAGERLPFPDETFEI
                     ILSHEVLEHVANDELAVQEMVRVLRRGGRIILFVPNRGYPFETHGVYWRGGYRFGNIP
                     LVNYLPRRLRDRLAPHVRVYTRSDLEKLFDGLPVRFVDRTIIFGAYDNIIARWPRLGR
                     LLRVLLHGLEKTPLRILGLSHFWVVEKTALEI"
     gene            3039..>5721
                     /locus_tag="AC16CB_04405"
     CDS             3039..>5721
                     /locus_tag="AC16CB_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341743.1"
                     /translation="MNLAFSEDIWKARIYRIYFILLVLQGSAALITLVSIPSDSRQAL
                     FWRLSWERLFLVGLLSAFVLTAGYLLIKVMRDSNWLRLLDQRLHAWLAYNQRWKKILF
                     LCVLSVLAGGLYLLVISSQSFYILAPAEIMDPSFNEYFVNQAYLRLQPYLVRILPIVT
                     LLIGTCAQTVILLPLAFFGVKAWHKQLKERNVYLMAGIYATLLILWQVVGWTQLKIEP
                     DYAFTGWYPLGAPILDNQVLLTFLVGTGLATFGIFLARVQKETSPGKNLAGMASRIDL
                     VVALALWVFAALHWLSIPTQTSWFVAGPRYPNYQYYPNSDAYLYDSTAQSLLVGSGFE
                     MGNREYARRPVYIMFLAGLYRLMGQDYETVTLVQSAIFAVFPVLIYWLTLTLHNRLSA
                     LIAALLVLFREGNAIALAERITVSNSRMFMAETPATIGVVLFACLVVWWLRQSGRRPV
                     IALILGGVLGVFMQIRIEVAFLLPSFLLLAGFVTCRAPKQWLKNAVLISLGMCLFLAP
                     WVWRNWHRTGLVFLERPAERLAFVAVRSQTGVGDLQTASAPAQDTLAAKINEASVFLR
                     RMSSHYINSQAQAFLIFPDAFRLMDSTIGYLEQGKSPRFWKACCSRQDYIDRFTFWMW
                     GKWEGAIPAQSVASIIINMLLVVIGLVKLWQWKRPAGIFLFIVSIGYYLATSAVRVSG
                     GRFVQVVDWIWIVCFSVGLAQIIEWTVASLSSANLPTWLFGEVHQAQIGWQGTPIQAI
                     TPLRTYMGVSLAIILAGASLPLVEAIIQPRYTEVARQAWLAELQQSELLEQNYPDVLE
                     TLKNIRRYRLIVLHGRALYPRYYASGEGESSTKITPFTPRDFARFSFYLVGPRNIGVL
                     LPLDEQPLVHFPNAEDVLVVGCMENGYLRALVVYLHSQ"
BASE COUNT         1194 a         1466 c         1596 g         1465 t
ORIGIN      
        1 aaatagcggg cggtgcgcaa atctggtccg atcaagaact ggacggcgcc aggaatcaga
       61 aacgacaatg gcatgtggtt gctccacggc ccgtaatcct ggtatggcca atactgtcct
      121 gttacaaaaa ggtaaccttt ataaaggtat gccccttcat ccagaatgga ttcctggcta
      181 tgtgcatata tcaccgattg cactaagtaa gcgatccccg ccaggacggc tattgcttct
      241 gctgtccagc gagaagagga aatacgccgt atggtggatt ttaggctcat gaaattcctg
      301 cggatacccg tctcaaatcg gcattgacca ttaattgaat taattcctgg aagctcacac
      361 gaggttgcca gcctagcttc tctttggcat tgcctgggtc ggctaccagc aggtctacct
      421 cggctgggcg gtaaaagcgc gggtcctgga ttacgtagtc attgtagtct aatcctagat
      481 agctaaaggc ggcttcacag aattcgcgca ccgagtgcgt gacgccagtc cccagcacgt
      541 aatcagccgg ttcgtcctgc tgtaacatca gccacatgcc ctcgacgtaa tcaccagcga
      601 agccccagtc tcgtcgcgct tccagattgc ccaggcgcag ttctttcgtc agacccagtt
      661 tgatacgggc cgcgttgtag gtgatcttgc gcgtgacaaa ctccaggccg cggcgcgggc
      721 tttcgtggtt gaataaaatg cccgaaacgg cgtataaatc atatgactca cgatagttga
      781 ctgtgatcca gtgcccgtac accttggcca cgccgtaagg gctgcgcggg taaaagggag
      841 tggtctcccg ctgaggcact tcgcgcacct taccgaacat ttcgctagaa gaagcctggt
      901 agaaacgcgc cttgggattt gaatggcgta tggcttctag tatgcgggtg actcctagcg
      961 cggttacctc gccggtaaga accggctggt tccaggaggt aggcacaaac gattgtgcgg
     1021 ccagattgta cacttcatcg ggctggtact cctccatgac gtcgaccagc gagctctggt
     1081 cgtgtaggtc tccctgaatg acggtgatat catcttggat gtgctggatg cgctcaaagg
     1141 tcacggtgct ggaacggcgt accatgccaa cgaccttgta tcctttggca agtaaaaaat
     1201 ccgccaggta tgagccatcc tggccggtta ttccggtgat gagagcaaca ggcataaggc
     1261 cttctccaat ataatattca acgggtgaat tataaccgtt gggtggggct ggtcaagagg
     1321 tacaaatatg gtatagtgag aatccgaaag gtatcccaga gacgatggca tatctcacag
     1381 gaggcgagcg ggcgcgttat gtgcagggta tgtttgcaca catcgcgcgc cgctatgatt
     1441 tgatgaaccg cctgatgacc gccgggcagg atgtgcgctg gcggcgcgag gtgatccgcc
     1501 gggcggagtt gcctgccagc ggtcgcttgc tggatctggg ggctggcaca ggcgatctgg
     1561 cgcgtgaagc gttgcgccag gtgccaaaga gtctgcccgt agcagccgat ttcaccttgg
     1621 aaatgatgcg agtgggcaag acacgcccca atatggggct ggaatgggca gcagccgatg
     1681 ccctgtgcct gcccttcccg gatgggagct tcgatgcagt cgtctcaggc ttcttgctgc
     1741 gtaatgtaag cgatatcctt cagtcgctca acgagcagcg ccgcgtgctt aagcccggtg
     1801 gacgtataat tgcacttgat accacccaac caccgaaaaa ccctcttgcg cctttgatca
     1861 agctgcacct gcacactgtt attcccacct tgggtcgcct gattgctggg tctgctgagg
     1921 cttatcgcta cttacccgaa accacggaga atttcctgag cgctgaaggg cttgcggagc
     1981 gcatgcagac cgctggtttc cagggggtgg gcttccgccg cctgatgttc ggcacggtgg
     2041 caatccactg gggattcaag tgagtccgga aaacggaatg tgaccaaatc cctcttaacc
     2101 atgtctctac ccaacgccag ttcctccagt gagtttcata ctgccaggtt tccctttgta
     2161 tccaagttta gaggggaaat cgagcgcaac gcgctgtaca tctttatctc acgctggacg
     2221 cgtctaatgg gcaaccgctc tggcttcaac ttcaatcagg gatggtacct gccttgagtg
     2281 ccgcatctcg cgattttgag aaagccgcct tgcgtgggga gccttcatac gtttggcgag
     2341 ctgggcaaga gcgccgccta aagatgatct tggcggcggc cggtgagcga gttaagggac
     2401 gcgtgctgga gaatggctgt ggggtgggca tgtatgccgc ccatttgttg ccccatgctg
     2461 gatcagtgac tggcctggaa tatgactttg agcgtgccgg ccaggcgagc aaagtccctg
     2521 gccaggtggt gaacgctgca ggagagcgtt tgcctttccc agacgagaca ttcgagatca
     2581 tcttgagcca cgaggtgctt gaacacgttg ctaacgatga gttggcggtg caagagatgg
     2641 tgcgcgtctt gcgccggggt gggaggatta tcctgtttgt ccccaatcgc ggctatccat
     2701 tcgaaacgca cggagtttac tggcggggag gctatcgttt cggcaacatc cctctggtga
     2761 attacctgcc gcgccgcctg agagacaggc tggcaccgca tgtgcgcgtc tacactcgta
     2821 gtgatttaga gaaactcttt gacggcttgc cagtgcgctt tgtggatcgc acaatcatct
     2881 tcggtgccta cgataatatc attgcccgct ggcctcgcct gggtcgcttg ctgcgagtgc
     2941 tcctgcatgg gctagagaag acaccgctgc ggatccttgg gctatcgcac ttttgggtgg
     3001 tggaaaaaac tgccctggag atataacgag aagagagtat gaatcttgcg ttttcggaag
     3061 atatctggaa agcccggatc taccggatct atttcatttt acttgtctta cagggtagtg
     3121 cagcactcat cacactcgtc tctatcccaa gcgattcgag gcaagccctt ttttggagac
     3181 tctcttggga gcgtctgttt ttagtgggat tactatctgc ttttgtgctg accgcgggtt
     3241 acctgctgat aaaagtcatg cgggattcaa attggctgcg gttgctcgac cagcgtctgc
     3301 acgcctggct tgcttacaac cagcgttgga aaaaaattct attcttgtgt gtgttgtcag
     3361 tcctcgcggg aggtttgtat ttgctggtga tctctagcca gagtttttat attcttgccc
     3421 cagcagagat catggatcca tcttttaacg agtattttgt gaaccaggct tacctgcgac
     3481 tgcaacccta tctggtgcgg atactcccta tcgtaacctt gctgattgga acctgtgccc
     3541 aaacggtgat cttgcttccc ctggctttct ttggggtgaa agcctggcac aaacaattga
     3601 aagagcgcaa tgtctatctc atggcgggta tctatgcaac gcttttgatc ttgtggcagg
     3661 ttgtaggctg gacacaattg aagatcgagc cggattatgc cttcacgggc tggtatcccc
     3721 tcggcgcgcc cattctggat aaccaggtcc tgctcacatt cctggttggg acaggcttag
     3781 ccaccttcgg catatttctt gctcgagttc agaaggaaac ttcacctgga aaaaatttgg
     3841 ctggtatggc atccaggatc gacctggttg tcgctcttgc cttatgggtc tttgcagcac
     3901 tacactggct ctctatcccc acccagacaa gctggtttgt tgcaggaccg cgttatccca
     3961 actaccaata ctacccaaac tccgacgcgt atttatatga ttcgactgca caaagcttgc
     4021 tggttggttc gggctttgag atggggaata gggagtatgc gcgccggccg gtatacataa
     4081 tgttcttagc cggcttatat cgattgatgg gccaggatta tgagacggtt acgctggtgc
     4141 aatcagcaat ttttgccgtc tttcctgtgt tgatttattg gcttaccctg acgctgcaca
     4201 atcgcctttc cgcactgatc gcggcgttat tggtgttatt ccgagaaggg aatgccatag
     4261 cgttggcgga aaggattacg gtctcgaact ccaggatgtt catggcagag acgcccgcca
     4321 ctatcggcgt ggttctgttt gcctgtctgg tcgtctggtg gctgcgtcaa tctggtcgca
     4381 ggccagtcat cgcgctcatc ttgggtggcg tgttgggtgt atttatgcag atccgcatcg
     4441 aggtcgcttt tttgttgccc tcctttctcc tgctggcagg atttgtcacc tgtcgtgctc
     4501 caaagcagtg gcttaagaac gccgttctga tttcgctagg gatgtgtttg ttcttagcac
     4561 cctgggtgtg gcgcaattgg caccgtactg ggctggtatt cctggagaga cccgctgaaa
     4621 ggctggcctt tgttgcggtg cgatctcaga ctggtgtggg cgaccttcaa acagcatctg
     4681 cccctgccca agatactctg gctgccaaga tcaacgaagc ctccgtcttc ttacggcgca
     4741 tgagttcgca ttacatcaat agccaagctc aggcgtttct gatctttcca gatgcctttc
     4801 gcctgatgga cagcacgata ggatacctgg aacaaggaaa atccccccgt ttttggaagg
     4861 cctgttgttc tcgacaagac tatatcgacc gcttcacttt ctggatgtgg ggcaaatggg
     4921 agggagctat tccggcccaa tccgttgcat cgatcataat caacatgctc ctggttgtca
     4981 ttgggcttgt caaactatgg caatggaaac gccccgcggg gatatttctg ttcatagtga
     5041 gcatcggata ttatctggcc acaagcgctg tacgtgtttc tggtggtcgt ttcgtccagg
     5101 tcgtcgattg gatatggatc gtttgcttca gcgttgggtt ggcacaaatt attgaatgga
     5161 cggttgcctc tttgagcagc gcaaaccttc caacctggtt atttggtgaa gtgcatcaag
     5221 cacagatcgg gtggcaggga acgcccatcc aggctataac accgcttcga acctacatgg
     5281 gggtcagcct ggcgatcatc ttggctggcg cttcccttcc attggttgag gccatcatcc
     5341 agccgcgtta cacagaggtc gcgcggcaag cctggctcgc tgagttgcag caatcggaat
     5401 tgctggagca gaattacccg gatgtgctgg aaaccctgaa aaatatccgg cgatatcgtc
     5461 tgatcgtcct gcatggtaga gcgctctatc cccgctatta tgcaagcgga gaaggcgagt
     5521 ctagcactaa aataacaccc tttacaccca gagattttgc gcgctttagc ttttacctgg
     5581 tcggtcctag aaacattggc gtactgcttc cattggatga acaaccgctg gtgcatttcc
     5641 ccaacgcaga agatgttttg gtcgttggtt gcatggaaaa cggctacttg cgggctttgg
     5701 tcgtctatct tcattcacag g
//
LOCUS       DBNGSX010000133        14247 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_772628, whole genome shotgun sequence.
ACCESSION   DBNGSX010000133 DBNGSX010000000
VERSION     DBNGSX010000133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14247)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14247)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14247
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_772628"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..3969)
                     /locus_tag="AC16CB_04410"
     CDS             complement(16..3969)
                     /locus_tag="AC16CB_04410"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3341744.1"
                     /translation="MKVSQNIQQFQPPRGILGKRRLNVSDFPETASSAIFIYQGNKFR
                     YVNKAMEAITGYTQPELLTMDFWDFIHPDYVDLIKYFSQQRNRRDKDSPSHFEIKIIN
                     KEGKERWLDFTPNLIEIQFEGRPAGLGTAFDISQRKNAEQSSSQTEVKLRRRAEELSV
                     LHSLSLDISAPLELPEMLQKIVKRSVELLNAAGGAISLCDDEHNKVVVQFENYHALEN
                     HHDVVFAYGEGAAGWVAQTGKPLIIDDYRIWDGRASVYEERQPYSAVLSVPMISQGKV
                     IGVLQVMADNMERLFDQSDQELLSLFANQAAIAVENARLLNQASIEKRHLSLLYDVGR
                     ELTTSLDPDEILNRAITLTCQALEGLVGQAFYYVPNNDLLSLRAIYGRPATLLKDFDE
                     QIAINPGRGLAGWTALHRQPVYVPDVTQDERWLFVQGIDDDARSAISAPILAGERLFG
                     VLSVLHVETGAFSSDQLNLLQGICQEVGLALSNATRYQQAQRQLAEITLIQNLAQTFN
                     RRLGLQALLNVIVTQLAERLGYPLVEIFLIEGDSLVQRAAYGVAHSQDLIPLTKGIIG
                     RVARTGQAALIPDVYLDPDYIPSFSDTVSELVVPIFHSTVVVGVVNIESRVPGQLTVQ
                     DRDLLEVLAVQVSIALENAVLYDHLVQHAADLEHKVVERTIELTELYELSQKIGYTLS
                     TRDLLQMLLSHLRNALRSDLVMGGMFSNGQRLLIVETNQPLAPDCVTLLHAHWLDMLQ
                     SQNISHKEYEDFQPEIVHAKTFDTSHEPLTHIDSLISSPITVAGDVVGLLIAGCAREN
                     AFAEAHIRILNTFANQAAVAFQRMAAILAAEQKRLEGLVEHLPVGILLLDSEFRLVVA
                     NPLGKEILAELSEEYAEGSLEYLGSQSVAELVARHNDQFPVEIVQRGTARRVFEAQAR
                     PAGKETGQWLLVLREVTYEREYQMRIQMQERLATVGQLAAGIAHDFNNIMAAIMVYTD
                     LLSDDPNIPPTSQDRLNTIQQQVQRATSLIRQILDFSRRSVMEQCDLDLLPFTKELDK
                     MLRRVLPETIQLELSYRSNKYMVRADPTRLQQALMNLALNARDAMPDGGLLQFELNRI
                     LIQPGERPPMEDMLPGEWIRFTISDTGQGISTDKLPHIFEPFFTTKPVGQGTGLGLAQ
                     VYGIIKQHDGFIDVKSRKGQGTTFSIYLPASSVPPVEEPVTKTKIQFSGGGRYVLVVE
                     DDQATREALEALLEAHQYSVVTSNNGAEALKLLEDGHTKIDLIVSDVVMPQMGGVALY
                     REVRNRWPQIKFLFITGHPMGGEYQDLLEKGKVLWLQKPFSAQSFNQSVHSLLEE"
     gene            complement(4084..5961)
                     /locus_tag="AC16CB_04415"
     CDS             complement(4084..5961)
                     /locus_tag="AC16CB_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0035999 - tetrahydrofolate interconversion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIR3341745.1"
                     /translation="MKRRFPVPSDIEIAQEAVLKPITQIADEVGLLAEEIELHGTYKA
                     KVKLSVLERLKDVPDGIYVDVTAITPTPLGEGKSTTMVGLSQALGAHLGKRVFTCIRQ
                     PSQGPTFGIKGGAAGGGYSQVVPMEDFNLHLTGDIHAITAANNLLAAAIDTRIYHETT
                     QKDDLKLFNALCPPDKEGNRSFSLSMLHRLKKLGIDKTDPDQLTDEEKSRFARLDIDP
                     DSITWRRVVDTNDRFLREITVGQGKEEKGRTRVTGFDITVASEIMAILALTTDLADMR
                     ERMGRIVIGTNRKGEAITAEDLGVAGAMTVLMKDAIKPTLMQTLEGTPVFVHAGPFAN
                     IAHGQSSIIADRIALKLADYVVTESGFGADIGMEKFFDIKCRYSGLIPNVVVMVATVR
                     ALKMHGGGPKVIAGKPLDPAYTDENLDLLRAGLPNLQHHIKNALRYGVTVVVAVNSFK
                     DDTQAEVELVRKAAIEAGAEDAVVCTHWMHGGEGAVKLAEAVVKAAEKPTNFKFLYPL
                     DISIKEKIETICKEIYGADGVEYLPEAEAKIELYTRLGFDKLPLCMAKTHLSLSHDPS
                     LKGVPNGFIVPIRDIRASVGAGFLYPLLGEMRTMPGLPTRPVFYDVDIDLETERVVGL
                     F"
     gene            complement(6135..6980)
                     /locus_tag="AC16CB_04420"
     CDS             complement(6135..6980)
                     /locus_tag="AC16CB_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3341746.1"
                     /translation="MSDSLLRLLLCTNGNENTRPALNYGVWMAGLLHSQVTLLGVIEE
                     LADEKIVKKLLDDTEEKLKDNDIPYDIRLDYGRGSVVVAKHASTGPFLTILGPLGRPA
                     WRRVIQGRSFRRVLEKVATPILYVRQVRPQLNKILLCLGGLEYSESVQQLCLYLAKIS
                     SASLTLLHVVEPVKLDYPISKEIHNHWQHILETDTPQGKTLRKAFEEAQSSGLPVQFK
                     VRHGNPVHEILEEVKSGGYDLIGMGSPHSAHSLRHLYMPNVTAEVAEVTDCPVLSVRK
                     GFSLV"
     gene            complement(6977..7495)
                     /locus_tag="AC16CB_04425"
     CDS             complement(6977..7495)
                     /locus_tag="AC16CB_04425"
                     /inference="COORDINATES: protein motif:HMM:NF014471.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HIR3341747.1"
                     /translation="MSRWMSLFWIFIKVNLLTTSGPASVGLLYKESVGKLMTESQFVE
                     AVGFSSVLPGSDALQLAMFVGYSAGGVPGALAALLGSILPPTLIMLVIASLLQRLRGE
                     AWIGSFVSGLTPAVAVLMVLIAWQIFNSEKNGYGLVTMGIAGLSLVALLLKAPPPLVL
                     LGAGLMGVIFFR"
     gene            complement(7522..9111)
                     /locus_tag="AC16CB_04430"
     CDS             complement(7522..9111)
                     /locus_tag="AC16CB_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006448 - regulation of translational
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongator complex protein 3"
                     /protein_id="HIR3341748.1"
                     /translation="MEYNNFRFRPHTPETLEIARLVLNDVHSGRKVLDAIRRHPLPGG
                     GYLSKSMLISAYKQLAESGIYEIDPSFLARIRMKPVRTLSGVTTVTVLTKPYPCPGKC
                     IFCPTDVRMPKSYLPDEPGAMRALQHDFDPYGQVVGRIQALQAVGHPTDKIELLVLGG
                     TWNSYRRDYQEWFIRRCFDALNGFASETLIDAHQYNETAAHRAVGLVVETRPDHVNPQ
                     EIAWLRYLGVTKIQMGAQSLDDRILLLNQRGHSVDDTHQAVALLRAAGFKIVLHWMPN
                     LLGATQDSDRLDFTRLWDNLCPDEIKIYPTQLLANADLYAYWQRGEYHPYTTEELINL
                     IADIKPSIPRYCRVNRVIRDIPSTNVVEGNKRTSLRQDVHQEMKRRGTRCQCIRCREV
                     RGQEVHPKKLRLDEYIYSAGGAQEYFISYTTPDDKLAAFLRLSLPRDGSPETGLIDLE
                     GAAIIREVHVYGQSLKVGAEQHGAAQHIGLGTRLLSHAEEIARSNGYSHLAVISAVGT
                     RRYYTRRGFERGELYLVKPID"
     gene            9098..9907
                     /locus_tag="AC16CB_04435"
     CDS             9098..9907
                     /locus_tag="AC16CB_04435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3341749.1"
                     /translation="MLYSIWRGRGKMVGLTYNIGVKSATAEKLIALNHQFYQTLALQF
                     SATRMRLQPGVRRILETLPSQVNILDLGCGNGELARELAKRDHQGFYMGLDFSSEMLA
                     EAQRSRLETLKAFFIQVDLSSPDWFTTIQRLQPGILEHPLDDILAFAVLHHLPGDDLR
                     KLVLRDVRKNLAPHGLFIHSEWQFLNSPRLRQRIQPWESIGLNESDIDEGDYLLDWRS
                     GGYGLRYVHHFTEQELMQLASETGFTVQQTFYSDGESGKLGLYQIWQPDLI"
     gene            9994..10722
                     /locus_tag="AC16CB_04440"
     CDS             9994..10722
                     /locus_tag="AC16CB_04440"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3341750.1"
                     /translation="MRILIISDIHSNLTALEAVLADAGQVDAVWCLGDLVGYGPDPNE
                     CISCVRELPNLACIIGNHDAASLNQIDSATFNPEARDAVRWTRDTLSDASVAFLQGLP
                     ERVEMDNVTLVHGSPRHPVWEYLLDTKNATQSFAYFESAYCFVGHTHLPSIYLLGTDN
                     HSARLMIPEANSRLSLSPRAILNPGSVGQPRDRDPRAAYAIYFPEEDIWEHRRATYQV
                     KSVQERMVAAGLPDRHIQRLAAGW"
     gene            10790..11740
                     /locus_tag="AC16CB_04445"
     CDS             10790..11740
                     /locus_tag="AC16CB_04445"
                     /inference="COORDINATES: protein motif:HMM:NF025616.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HIR3341751.1"
                     /translation="MIKRILPLILTTSLLWISACASARSLAPEAGAPPFEELAAREMA
                     EPASAVFMDDAQGEIMPAVGQTQTERLVIKNADLTIVVDDPVKSMDNITYLAEEMGGF
                     VVSANLREMTLDSGAKSPRASITIRVPAADFNDALTRIKEETEQLPLNENISSQDVTS
                     EYTDLQSRLRNLEATEDQLTEIMGSANKTEDVLSVYNRLVQVREQIEVIKGQIQYYEQ
                     SAAMSLISVELIANEAVQPLTIGGWQPVGTAKDAVQTLINTLKVLGNLVIWIILYVLP
                     VLVVLFVIFVLPLRLVWQAWRRRKAKGKESLPPAPPSQST"
     gene            complement(11841..12854)
                     /locus_tag="AC16CB_04450"
     CDS             complement(11841..12854)
                     /locus_tag="AC16CB_04450"
                     /inference="COORDINATES: protein motif:HMM:NF024919.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HIR3341752.1"
                     /translation="MQPAGKQTGVSPKTSAPGAPVPSYSRTHYNWGNHGFGKKIRHLL
                     ALVFLGILLPVTRPLSAKADHLPERAYIRGVIGHAQSYPLSCESRSAADWAAFWDVNI
                     RERKFLTKLPRSENPDEGFVGNPSDAWGNIPPKSYGVHAEPVAALLREHGLQAEARRG
                     MQWDELRAEIAAGRPVIVWVIGQIWQGTPIKYSAPDGHKTTVARFEHTMIVIGYGPSV
                     VHLVDAYSGKTVSHPLRRFLASWKTLGNMAVVGQANPEEPSKPEQGNSSQVFLPVIIS
                     GTSETSQSVSPTPSPKTYVVKRGDYLVGVARRFSVNWRKLANLNGIKYPYTIYPGQVL
                     KIP"
     gene            12785..13495
                     /gene="rsmG"
                     /locus_tag="AC16CB_04455"
     CDS             12785..13495
                     /gene="rsmG"
                     /locus_tag="AC16CB_04455"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR3341753.1"
                     /translation="MRELARLVHSFLGIRLSASQLAALTSYEAELVEWNSRFNLTAID
                     TPENIRIKHFLDSLTCLLVMKDRPMERIIDIGTGAGFPGLPIKIVYPSVALTLVDSVG
                     KKVDFCRHIAQKLGLENVDIIQRRAEELGQLPEHREQYDWAVARAVAVLPVLVEYMLP
                     LVRVEGGMIAMKGESAPVEAHSADNALRLLGGRLKNLVPVTLPGVADERYLVVVDKVV
                     ATPLQYPRRVGIPAKRPL"
     gene            complement(13523..14023)
                     /locus_tag="AC16CB_04460"
     CDS             complement(13523..14023)
                     /locus_tag="AC16CB_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR3341754.1"
                     /translation="MSLNNPTMGERVGWIFQINASNGGVPKLSRKLGEVTFNGLNGDN
                     QANPDVHGGPERALCLYSLERILALQAEGHPIFPGSAGENLTLAGLDWSLVNPGTRLQ
                     LGADVLVQVTRYTSPCNTISGSFLKNDYSRISQKVFPSWSRVYAQVLQPGTIQVSDAV
                     RISTLD"
     gene            complement(14010..>14247)
                     /locus_tag="AC16CB_04465"
     CDS             complement(14010..>14247)
                     /locus_tag="AC16CB_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIR3341755.1"
                     /translation="EVAWLKEETGQWPVLLLDEVLAELDVQRRTDLLERLADIEQAML
                     TTADLEAFSKDFIRQATLWRIYAGQLEELKHVPE"
BASE COUNT         3407 a         3513 c         3727 g         3600 t
ORIGIN      
        1 gctggggttg tagggttatt cttccagaag tgagtggaca gactgattga agctctgggc
       61 tgagaatggc ttttgcagcc acaggacctt gcctttttct agcaaatctt ggtattctcc
      121 gcccatgggg tgtccagtga taaacaggaa tttgatttga ggccagcgat tccggacttc
      181 cctgtagagg gcgacaccac ccatttgggg cattactaca tcgcttacga tcagatcgat
      241 tttcgtatgc ccgtcctcca ataactttag cgcttccgcg ccattgtttg atgttactac
      301 ggagtattgg tgagcttcga gcaatgcttc taaggcttcg cgagtagctt ggtcatcttc
      361 gactactaaa acatatcttc ctcccccact aaattggatc ttggttttcg tgactggctc
      421 ttcaaccgga ggcacgctac tggcagggag ataaatcgaa aatgtggtgc cttgtccctt
      481 gcggcttttg acatcaataa agccatcgtg ttgcttaatg atgccataga cctgtgcaag
      541 acccaatcct gttccctgcc cgacgggttt ggtggtgaag aaaggctcaa atatgtgggg
      601 gagtttatct gtagagatac cctgcccggt atcgctgatg gtgaaacgaa tccattcccc
      661 cggaagcatg tcttccatgg gaggcctctc gcctggttgg attaggatac gattcagctc
      721 aaattgtaat aaacctccat caggcatggc gtctcgggcg tttaaagcca ggttcatgag
      781 agcctgttgt aaacgggtgg gatcagctcg aaccatatac ttatttgaac ggtaggataa
      841 ttccaactgg atagtctctg gtagcacgcg ccgcagcatc ttatccagtt ctttggtgaa
      901 aggtagcaaa tccaagtcac actgctccat taccgagcga cggctgaagt ccaagatctg
      961 gcggatcaaa ctggtagcac gttgcacttg ctgctgaatg gtgtttagtc gatcttggct
     1021 ggtaggagga atgttcgggt cgtcactcag caagtctgta taaaccatta tggctgccat
     1081 aatgttgtta aaatcgtgcg cgatgcctgc tgcaagttgc ccaacagtag ccagtcgttc
     1141 ttgcatctgg atgcgcattt gatactcgcg ctcgtaggtc acctcacgca agactaataa
     1201 ccactgacct gtttcttttc ccgctgggcg tgcctgggct tcaaagactc gccgagctgt
     1261 accgcgctgc acaatctcga caggaaattg gtcattatgg cgggctacga gttcagcaac
     1321 actttgagac cctaagtatt ccagggatcc ctcggcatac tcttcactga gctcagctag
     1381 gatctccttg ccgagcggat tagccaccac cagccgaaat tcagaatcca ataataggat
     1441 acccaccggt aagtgttcaa ccaaaccttc caggcgtttc tgctctgcag ccagaatagc
     1501 agccatgcgt tgaaaagcga ctgcagcctg gttagcaaaa gtattcaaaa tgcgtatgtg
     1561 cgcttcggca aaggcatttt ctcgtgcaca cccggcaatc aacagaccga ctacgtctcc
     1621 ggcgactgtg atgggtgagc tgatcagtga gtcgatatgc gtcaaaggct catgactggt
     1681 gtcgaacgtc ttcgcatgga cgatctctgg ctgaaagtct tcatattctt tgtgcgatat
     1741 attttggctt tggagcatgt ccagccagtg agcgtgcaaa agagttacgc aatcgggtgc
     1801 cagaggctgg tttgtctcga cgatgagcaa tcgctgtcca ttactaaaca tgcctcccat
     1861 cactaggtcg ctgcgtagcg cattgcgcag gtggctaagc agcatttgga gcaaatccct
     1921 agttgatagc gtatagccga ttttttgact taattcatac agctcggtaa gctcaatcgt
     1981 gcgctccacc actttgtgtt ccaggtcggc ggcatgttgc acaaggtgat catataaaac
     2041 cgcgttttct aatgctatcg aaacctggac tgccagcacc tcaagcagat cgcgatcttg
     2101 gacggtcaat tgtccaggaa ctcgagattc aatgtttact accccaacca ccactgttga
     2161 gtgaaatatc ggaacaacca gttcagagac tgtgtccgag aagctaggga tatagtctgg
     2221 atcgaggtat acatcgggaa tgagagcggc ttgtcctgtg cgcgctacac gcccaatgat
     2281 gcctttggta agcgggataa gatcttggga atgggcgact ccataggcag cacgttggac
     2341 cagagagtca ccttcgatga ggaaaatctc tactagcgga tatcccaagc gttcggctaa
     2401 ttgcgtgaca atgacgttga gtaatgcctg cagacctagt cgtctattaa atgtttgcgc
     2461 gaggttttgg atgagggtga tctcagctaa ttggcgctgg gcttgctggt aacgcgttgc
     2521 gttgctgaga gctaatccca cctcctgaca gataccttgt aataaattca gttgatcaga
     2581 ggaaaatgcg ccggtctcga catgcagcac cgagagcaca ccaaatagtc gctcgccagc
     2641 gagaatggga gcgctgatcg ccgagcgggc atcatcatca ataccttgaa caaatagcca
     2701 acgctcatct tgggtgacat cgggcacgta aactggttgg cgatgcaaag cagtccaacc
     2761 cgctaatcct cggccaggat taatggcaat ttgctcgtcg aaatctttaa gcaaggttgc
     2821 tggacggcca taaatggcac gcaggctcaa aaggtcatta ttgggaacat agtagaacgc
     2881 ctgaccaact aatccttcca gagcctggca tgtaagagta attgctcggt ttaggatttc
     2941 atctggatca aggctggtgg ttaactctcg ccctacatcg taaagcaggc tgaggtgacg
     3001 cttctcgata gatgcctggt ttaagagacg cgcattctcg accgcgatgg cggcctgatt
     3061 ggcaaataat gacagtaatt cctgatcgga ttgatcaaag agacgctcca tattgtcagc
     3121 catcacctgt aagacaccaa taacctttcc ttgggaaatc atgggtacac tgaggacagc
     3181 ggaatagggt tgcctttcct cgtaaactga tgcgcgacca tcccagatgc ggtaatcgtc
     3241 gataatcaat ggtttgcctg tttgggcaac ccacccagcc gctccctctc cataagcgaa
     3301 aaccacatca tgatgatttt ctagagcgtg ataattttcg aactgtacga ctaccttatt
     3361 atgctcatcg tcacacagag agattgcgcc accagctgca tttaaaagct ctacagagcg
     3421 cttgacaatc ttttgcagca tttcggggag ttcgagcggt gcagagatgt ccagcgagag
     3481 agagtgaaga actgaaagtt cttcggcgcg acggcgtaat ttaacctcag tttggctgct
     3541 ggattgctca gcgttctttc tctgggaaat atcgaaagct gtccccagac cagctggtct
     3601 gccttcaaac tggatttcaa tcaggttagg agtgaagtct aaccagcgtt ctttcccctc
     3661 cttattgata atcttgattt caaaatgtga gggactgtct ttatctctgc gattacgctg
     3721 ctggctaaaa tatttaatca aatcaacata atcagggtgg ataaaatccc agaaatccat
     3781 ggtgagcaat tctggttgtg tatagcctgt gatggcctcc atagctttgt tgacgtagcg
     3841 gaacttattc ccctgataaa tgaaaattgc cgaggaagct gtctcgggaa aatcgcttac
     3901 attgagtctg cgcttaccca ggatccccct tggaggctga aattgttgaa tattctggct
     3961 caccttcatg gctatagcat tatagagtaa attatttggt ttttgcctta catattcgtt
     4021 gagacaaaag aagagcgggc ggattttccg cccgctcacc atcatatgga ggtaggttcg
     4081 agtttagaat aatcccacga cgcgctcggt ctcaaggtca atatcgacat cgtagaaaac
     4141 cgggcgggtt ggcaagccag gcatggtgcg catctcaccc agcagggggt aaaggaatcc
     4201 ggctccgacc gaggcgcgaa tatcgcgaat cggcacgatg aagccatttg gcacgccctt
     4261 caagcttgga tcatgactca aactgaggtg ggtttttgcc atgcacaagg gcagcttgtc
     4321 aaatcctaaa cgcgtgtaga gttcgatttt ggcctcagcc tctggtagat attccacacc
     4381 atcagcgccg tagatttcct tgcaaatagt ctctatcttc tctttgatag aaatatccag
     4441 cggatatagg aatttgaaat tagtcggttt ctctgcggct ttaaccacag cctcagccag
     4501 cttgactgcc ccttcaccac catgcatcca gtgagtgcag actaccgcat cttcagcacc
     4561 tgcttcgata gcggccttgc gcaccagttc cacttctgct tgggtgtcat ctttgaagga
     4621 gttgacagcc accactaccg tgacgccata tctcagggca ttcttaatgt ggtgctgcaa
     4681 gttgggcaag ccggcgcgca gcaaatccaa attctcatcg gtataggctg gatcaagtgg
     4741 cttcccagct attaccttgg gaccgccacc gtgcattttc agggcacgga cggtcgccac
     4801 catcaccaca acattaggga tcagacctga atatcggcac ttgatgtcga aaaacttctc
     4861 catgccaatg tcggcgccaa aaccagattc ggtcacgaca taatctgcta gttttagggc
     4921 gattcgatcg gcgataatgg agctttgtcc gtgagcgata ttggcaaatg ggccagcatg
     4981 cacgaagacc ggtgtgccct ccaaagtttg catcaaggtt ggtttgatcg catctttcat
     5041 caagactgtc atcgcaccgg cgacccccaa atcttctgcg gtgatggctt cgcctttcct
     5101 gttcgtgcca atgacgattc ggcccatacg ctcacgcata tcagccaggt cagtggttaa
     5161 agccaagatc gccatgatct cactggctac tgtgatatcg aagccagtta cgcgagtcct
     5221 gcctttctct tccttacctt gaccgacggt gatttcacgc aggaagcgat cgttggtatc
     5281 aactacccgc cgccaagtga tgctatcggg gtcgatatcc aggcgggcaa agcggctctt
     5341 ctcctcatcg gttaattgat caggatcggt cttgtcaatg ccaagtttct tcaagcggtg
     5401 taacatggag agagagaaac tgcgattacc ctccttgtct ggcgggcaca aggcgttgaa
     5461 aagcttgaga tcatcctttt gggtggtctc atggtatatg cgcgtgtcaa tggcggctgc
     5521 cagtagattg ttggcagcag taatggcatg gatatcgccg gtgaggtgca ggttgaagtc
     5581 ctccattggg accacctggc tgtatccacc accagcggcg ccgcctttaa tgccaaaggt
     5641 tggaccctgg ctgggttggc gaatgcaggt gaacactctt ttgcctaggt gtgcgcccag
     5701 agcctggctt aaacccacca ttgtcgtgct ttttccttca ccgagtggtg taggtgtgat
     5761 cgctgtcaca tccacgtaga tgccatcagg gacatctttg aggcgttcaa gcactgagag
     5821 ctttactttg gccttatacg tgccatgcag ctcaatttct tctgctaata ggccaacctc
     5881 gtcggcaatt tgggtgatgg gttttaagac ggcttcttgg gcgatctcga tatcggaagg
     5941 gacgggaaag cgccgcttca ttttgttcgg tgtgaagcga cgtagaccag ggaaatcttt
     6001 agcagcagac atacactctc ctttgtatag gtattttgat gatttttatt aacgtatcta
     6061 ttgtgacagg tttcattaat ctaatcaaga gacgcatatc attttcgttt atgccgagtg
     6121 tcacaatttg gtgttcagac gagtgaaaaa ccctttcgta cagacaacac tggacaatcc
     6181 gtaacttcag cgacctcagc ggtcacattg ggcatatata aatggcgtaa actatgtgcg
     6241 ctatgtgggg agcccatccc tatcaggtca tatcctcctg atttcacttc ttctaaaatt
     6301 tcgtgtactg gattgccatg tcgaacttta aattggaccg gcaaccctga ggattgcgct
     6361 tcttcgaaag ctttccttag ggtctttccc tgaggtgtat ccgtttccaa aatgtgctgc
     6421 cagtggttat ggatctcttt ggagatcggg taatcaagtt tcacgggctc aacgacatgc
     6481 agcaaagtca aactggcact actgatcttt gctaaataga ggcagagttg ttgaacactc
     6541 tcggagtatt ccaaacctcc caaacagagc aatattttat tgagttgcgg tctaacttgg
     6601 cgcacataca atatgggtgt ggcgaccttc tctaacacac ggcggaagga gcgtccctgg
     6661 atcactcgac gccaggcagg tctgccaagc ggacccaaga tggttagaaa gggtcccgtg
     6721 ctggcatgct tggcaacgac caccgatccc ctgccataat ccaaacggat gtcatacggg
     6781 atatcgttgt cctttagctt ttcttcggta tcatcaagga gttttttgac gatcttttcg
     6841 tccgctagct cttctatcac gcccaatagc gtgacttggg agtgtaataa ccctgccatc
     6901 cacacaccat aatttagtgc gggacgcgta ttttcgttgc cgttggtaca caaaagcaag
     6961 cgtagcagac tatcgctcat cggaaaaaga tcactcccat caggccagca ccgagcaaca
     7021 ccagtggggg gggagctttg agcagcaaag cgaccaggct cagccctgcg atacccattg
     7081 ttaccagacc ataaccattt ttttcgctgt tgaagatctg ccaggcgatc aacaccatca
     7141 ggactgccac tgctggggtt agcccgctca caaaactgcc aatccaagcc tcgccgcgca
     7201 gacgctgtag taacgaagct atcaccagca tgatcagcgt gggcggcaga atcgaaccta
     7261 gcaaggctgc cagagcacct ggcacgccgc ctgcgctata gcccacgaac atcgccagtt
     7321 gaagagcgtc gctgcctggc agtacactcg aaaagcccac agcctccacg aactggcttt
     7381 cagtcatcag ttttccgact gactctttat ataacaaccc aaccgaggca gggcctgagg
     7441 tagtcagcag gtttacctta atgaatatcc aaaatagact catccagcga gacacaagga
     7501 gatctccttt gtttttgtag attaatcgat cggtttaacc agatacaatt ccccccgctc
     7561 aaatcccctt cttgtgtaat aacgccttgt tcccactgcc gagatgactg ctaaatggga
     7621 gtagccgtta gagcgagcta tctcttcagc atgtgaaagc agacgcgtgc ccagacctat
     7681 gtgctgggcg gcaccatgtt gctctgcccc tactttcaat gattgaccgt aaacgtggac
     7741 ctcgcggatg atcgccgctc cttccaggtc gatcagcccc gtttcgggtg aaccatctct
     7801 aggtagcgag agtcgcagaa aagcagccaa cttatcatct ggggtggtat atgagataaa
     7861 gtactcctga gctccaccag cgctataaat gtactcatct aaacggagct tttttgggtg
     7921 tacttcctga ccgcgaactt ccctgcagcg aatgcattga cagcgagtgc cgcgtcgttt
     7981 catttcttgg tggacatcct ggcgcaggct ggtgcgcttg ttcccttcaa caacgtttgt
     8041 agaaggaata tcgcgtataa cgcggttcac gcggcagtac cgcgggatgc tgggtttaat
     8101 atctgctatt aaattaatca gttcttcagt ggtgtacggg tggtattcac cacgctgcca
     8161 ataagcatat aagtcagcgt tggcgagcag ttgggtggga tagatcttta tctcatcagg
     8221 acatagattg tcccacaagc gggtgaaatc taaacggtcg gaatcttgtg tagcgcccaa
     8281 tagatttggc atccagtgca acacaatctt gaaaccagca gcacggagga gagccaccgc
     8341 ttggtgcgtg tcatcgacgc tatggccgcg ctgattgagg agcaggatgc gatcatccag
     8401 gctctgagcg cccatctgta ttttggtgac gcccagatat cgtaaccagg cgatctcctg
     8461 ggggttgaca tgatcggggc gtgtctccac gaccaatccc acggcgcgat gagcggctgt
     8521 ttcattatac tggtgcgcgt ctatcagagt ttctgaggcg aagccgttga gtgcatcgaa
     8581 gcatcgacgg ataaaccatt cctggtaatc acggcggtag gagttccatg tcccacccaa
     8641 gaccagtaac tcgattttat ccgtggggtg gcctacggcc tgcaacgcct ggatccgccc
     8701 cacgacctgt ccatagggat caaaatcgtg ttgcagggcg cgcatagcac ctggctcgtc
     8761 tggcaggtaa ctcttgggca tgcgcacatc ggtggggcaa aagatacact ttccggggca
     8821 tgggtagggc ttggttaata cggttaccgt ggtaactccg gagagtgtgc gcacaggttt
     8881 catgcgaatg cgcgccagga aagatgggtc aatttcgtag atgccgctct cggcgagttg
     8941 tttataggct gagatcagca tggatttact cagataacca ccgcctggca gtgggtgtcg
     9001 ccgaatggcg tccagcacct ttcgaccgga atggacatcg ttgaggacca aacgggctat
     9061 ttcgagggtt tctggagtat gtgggcgaaa gcgaaaattg ttatattcca tctggcgcgg
     9121 acggggaaaa atggtcggtc tgacttataa tatcggtgtg aagtctgcca ctgcagagaa
     9181 attaattgca ctcaaccatc aattttacca gaccttagca ctccagtttt cagccacccg
     9241 catgcgcttg cagcccggcg tgcggcgcat cctggaaacc ttgccaagcc aggtgaacat
     9301 cttggacctt ggatgtggaa acggagaact agctcgtgaa ctggcaaaac gcgatcacca
     9361 ggggttctat atggggctgg actttagcag tgaaatgctc gcagaagccc aaaggtcccg
     9421 tcttgagacc ctgaaggctt tttttattca agtagacctg tcctccccag attggttcac
     9481 aactatccag cggttacaac ccggcatttt ggaacatccg ctggatgaca tcctcgcatt
     9541 tgccgtattg caccatctgc ctggggatga tttgcgcaaa ttggttttac gggatgtgcg
     9601 caaaaacctt gccccgcatg ggctgtttat ccactcggaa tggcagtttt taaacagccc
     9661 tcgattacgc cagcgcatcc agccgtggga gagcatcggg ctcaacgaaa gtgatataga
     9721 tgaaggtgat tacctgttag attggcgcag tggtgggtat ggtttgcgct atgtgcatca
     9781 ctttacagaa caggagttga tgcaattggc aagtgagacg ggcttcaccg tgcaacagac
     9841 tttttattct gatggtgaaa gcggcaagtt gggtttatac caaatctggc agcctgattt
     9901 gatttgagcg catcgttatt gatttgttac agggtggttt gtcgactgat ctcttaacga
     9961 tatgcttatt ctgtgctact attaggtgtt cctatgcgaa tcttgattat ttcggacatc
    10021 cattccaacc tgactgcttt ggaagccgtg ctggcggatg ctggacaggt tgacgcggta
    10081 tggtgcctgg gggatttagt gggttatggt ccagacccaa atgagtgtat ttcctgtgtg
    10141 cgtgagctgc ccaacctggc atgtatcatc ggcaatcacg atgcagcatc gctcaaccag
    10201 atcgattcgg ccactttcaa tccagaagcc agagatgcag tcagatggac ccgggatacc
    10261 ctatctgatg ctagtgtagc attcctccag ggcttacctg agcgggtcga gatggataat
    10321 gtcactttgg tgcatggcag ccctcggcat ccggtttggg aatatttatt ggatacaaaa
    10381 aatgcaactc agagttttgc ttacttcgaa tcagcatatt gttttgttgg gcacacccac
    10441 ctgcctagca tatatctact tggcacggac aaccattctg ccaggttgat gatcccagag
    10501 gccaactcgc gtttgtccct ttcgcctcgg gctatcctaa atcccggatc agtgggccag
    10561 ccgcgagatc gagatccgag ggccgcctat gcgatttatt tcccagagga ggatatctgg
    10621 gaacaccgtc gagcgactta ccaagtcaaa tctgtacagg aacgaatggt ggcagctggt
    10681 ttgccagatc gccatatcca acgccttgct gcgggatggt aaggggagtg gggaacattt
    10741 tgattgcggg ggacgtcttg ggagaaatag ggtgaaagga gttatggcaa tgatcaaaag
    10801 aattctccct ttgatattaa caacgagctt attatggatt tcagcctgcg catcagcgag
    10861 atccttggcc cctgaagctg gcgcgccgcc tttcgaagag ctggctgcaa gggagatggc
    10921 agagccagcc tccgctgttt ttatggatga tgcacagggt gagatcatgc ctgctgtagg
    10981 tcagacacag accgagcgct tggtgataaa gaatgccgat cttaccattg tggtagacga
    11041 ccccgtgaag tctatggaca acattaccta cctggctgag gagatggggg ggttcgtggt
    11101 ttcggctaat ctgcgcgaga tgacgctgga cagcggcgca aaatctccgc gggcttctat
    11161 caccattcga gtcccggctg cggatttcaa cgatgccctg acccgtatca aggaggagac
    11221 agagcaactc ccgctcaatg aaaatatcag cagtcaagat gtaaccagcg agtacaccga
    11281 tttgcagtcg cgtttgcgca acctggaagc caccgaagac cagcttacgg agataatggg
    11341 ttcagcgaac aagactgaag atgtgctatc ggtttacaac cgattggtgc aggtacgtga
    11401 gcagatcgag gtgataaagg ggcagataca atattatgag caatcagcgg cgatgtcgct
    11461 catcagtgtt gaattaattg ccaatgaggc tgttcagccg ctcaccatcg gtggatggca
    11521 gccagtgggc accgccaaag acgctgtcca gacattgatc aacaccctga aggtgcttgg
    11581 aaacctggtt atttggatta tcctgtacgt gctgcctgtg ttggtagttt tatttgtgat
    11641 ttttgtgctg ccgcttcgat tggtttggca agcctggcgg cgccgcaagg cgaaaggcaa
    11701 ggagagcttg cccccagcac ctccttcaca atcaacgtaa tattacctct ccatttcaac
    11761 gtcctctgta cggtagagag ataaataatc gaagaaggta tctgcaaggg tttacacccg
    11821 ccgcagatac cttttgctat ctatggaatt tttagaactt gacctggata aatggtatac
    11881 gggtatttga tgccattcag attagcaagt ttgcgccagt tgacgctgaa tcgccgcgcc
    11941 actccaacca ggtaatcgcc gcgtttgacc acgtaggttt tagggctcgg ggttggagaa
    12001 acggattggg aagtttccga agtacccgaa attataactg gtaggaatac ctgggacgaa
    12061 tttccctgct cgggtttcga tggctcttcg gggttggctt gcccaacaac agccatattt
    12121 cctagcgtct tccaggaggc taggaatctg cgcagcgggt gagataccgt cttaccacta
    12181 taagcatcta ccagatgtac aaccgatgga ccgtaaccga tgacaatcat ggtgtgctca
    12241 aagcgggcga cagtggtctt gtgcccatcg ggtgcggaat atttgatcgg agtgccctgc
    12301 catatttgac cgatgaccca tacgatcacc gggcgccccg cagcgatctc agcgcgcaat
    12361 tcgtcccatt gcatgcctcg ccgagcttct gcctgaagac catgctcacg caacagagca
    12421 gctactggct cggcatggac gccataagat ttgggcggga tgttccccca ggcatcactt
    12481 ggatttccca caaagccttc gtcggggttt tctgaacggg gcagtttggt aagaaacttc
    12541 cgctcacgga tattgacgtc ccaaaatgca gcccaatcgg ccgccgagcg cgactcgcac
    12601 gatagcgggt agctctgagc gtggccgatt accccgcgta tgtaggcgcg ctctggaagg
    12661 tgatcagctt tagcagatag ggggcgggtg acgggaagaa gtataccgag gaatacgagc
    12721 gctaggagat ggcgtatttt tttcccaaag ccatgattcc cccagttata atgggtacgt
    12781 gagtatgagg gaactggcgc gcctggtgca ctcgtttttg gggatacgcc tgtctgcttc
    12841 ccagctggct gcactgactt cctatgaagc agagttggtg gaatggaatt caagattcaa
    12901 cctcactgct atagacactc cagagaacat ccgcatcaag cactttcttg actccttaac
    12961 ttgcttgttg gtgatgaaag atcgacccat ggagcggatc attgatatag ggactggagc
    13021 tggctttcca ggattgccaa ttaagatagt ctatccatct gtggcattga ccttggttga
    13081 ctcggtgggg aagaaggtcg atttttgtcg gcatatagct cagaaactag gattggaaaa
    13141 tgtcgacata atacagcgcc gtgccgagga actgggtcag cttccagagc atcgtgagca
    13201 atatgattgg gctgttgcca gggctgtggc agttctaccc gtgctggtag agtatatgct
    13261 gcctctagtg agggtggaag ggggtatgat cgctatgaag ggtgaaagtg ctcccgttga
    13321 agcacattca gcggataatg ccctgcgcct gttgggtggc aggctcaaga atctggtacc
    13381 tgtcaccttg cctggggtcg cagatgagcg atatctggtg gtggtggaca aagtggtagc
    13441 tacaccactg caatatccca gacgagtggg tatacccgcc aaaaggccat tataggcaaa
    13501 aattgattcc tttatttctt tatcagtcca gtgtagaaat gcgtactgca tcactcacct
    13561 ggatcgtgcc cggttggagg acttgcgcat agacacgcga ccagcttgga aagaccttct
    13621 gggagatacg tgagtagtcg ttcttcagaa atgaaccgga aatggtattg cagggggatg
    13681 tgtagcgagt cacttgcact agcacatcgg ctcccaattg gagacgggtg cccggattga
    13741 cgagagacca atccagacca gccaaagtga ggttttcacc agctgatccc gggaatatgg
    13801 gatgtccttc agcttgcaag gcaaggatac gctccaggga gtaaaggcaa agcgcccgtt
    13861 ctggtccacc atgaacatcc gggttggcct gattatcccc atttagaccg ttgaaggtga
    13921 cttcgccgag ctttcggctt aacttgggca caccgccatt tgaggcgtta atttgaaaaa
    13981 tccaaccaac gcgctctccc atggtcgggt tattcaggga catgcttcaa ctcctcgagc
    14041 tggccggcgt agatacgcca gagggtggcc tgacggatga aatccttaga gaaagcttcc
    14101 agatcagcag tggtaagcat agcctgttca atatctgcca agcgctctaa taggtcagtg
    14161 cggcgttgta catcgagctc agccaggact tcgtctaaca agagaactgg ccattgaccg
    14221 gtctcttctt ttagccaggc cacctcg
//
LOCUS       DBNGSX010000134         4267 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1741809, whole genome shotgun sequence.
ACCESSION   DBNGSX010000134 DBNGSX010000000
VERSION     DBNGSX010000134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1741809"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="AC16CB_04470"
     CDS             <1..552
                     /locus_tag="AC16CB_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3341756.1"
                     /translation="SLDFHSPYGCSKGSGDQYTRDYYRIYGLPTVVFRQSCIYGPRQF
                     GIEDQGWLAWFMIAVVASRPITIYGDGKQVRDVLFIDDLLDAYDAALERIDIAAGQVY
                     NIGGGPDHAIAVWTEFGALLENLMGQKIHPKMSDWRPGDQKIYVSDIRKAERDLAWAP
                     KIGVKKGLKRLYEWVLENRELFR"
     gene            558..1718
                     /locus_tag="AC16CB_04475"
     CDS             558..1718
                     /locus_tag="AC16CB_04475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3341757.1"
                     /translation="MRILTVLTYYRPHTSGLTIYAERLARALARRGHQVTVFTTQYDG
                     SLARDEMQHGVRVVRAPVALRISKGVIAPAFGFMATRLVQEHDVVHLHLPQFDAPGVA
                     LRGRLLRKPTVLTYHCDLKLPPNPFNYFLNRGVHLVHHMAAKLANRIVTYTQDYADNS
                     RYLSNYKHKLQIIPPPVELPAVPHGDVEAFAEAHNTSERRPVIGMAARLATEKGVEIL
                     LEALPSIIEKFPRAQVLFAGQYEDVLGEREYYNRLMPVIRGYQQQGQWEFLGVLNPNQ
                     MAAFYPNLDVLVVPSLNSTEAFGLVQIEAMMHGVPCVASALPGVRQPILTTGMGKVVP
                     IGDSGALANAIQAILAEPDQYQKDPRAIAQRFTPDAIALLYEEMFEDMLAKK"
     gene            1739..2530
                     /locus_tag="AC16CB_04480"
     CDS             1739..2530
                     /locus_tag="AC16CB_04480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3341758.1"
                     /translation="MQEKDFLWLHLRDLPYFRALVRAVEAEYYQDIELPSPQLDIGCG
                     DGHFASLVFEKPVDVGVDPGGTAIRQAARLGSYRSLVQSDAGALPFPEGYFGSAFSNS
                     VLEHIPHVRDVLAETARVLKPGALFLFCVPNPRYLSELSVPGILARARLNGLGKAYTE
                     WFRRISRVQHAQPPEVWCLWLEAAGFTVEKWWHYFSPAALHMLEWGHYFGAPSLLPHV
                     LTGRWILARTRWNLALTERIARPYAQPNPHPQGTFTFYITRKVAG"
     gene            2551..3282
                     /locus_tag="AC16CB_04485"
     CDS             2551..3282
                     /locus_tag="AC16CB_04485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3341759.1"
                     /translation="MTGSSNPYDDTYFMRAAYANVSFARFSQYWWSNRYYALLARRHG
                     PPNGRVLEVGCGLGHLLGWLTDSYQVFGVDINSWALAQARQNVPQGHFAYLSAEDLGA
                     FQDGSFQIVVAKHVVEHLPDPDHALKEMSRVLAKDGLLLLATPNLDSLMRSVKKERWI
                     GYQDPTHINMKPPDEWIGSLHRYGLWPKRVFSDGFWDAPYINWLPTRLQKLIFGAPGG
                     LQAILGWSIIPLRMGESMIVLAKKS"
     gene            3222..>4267
                     /locus_tag="AC16CB_04490"
     CDS             3222..>4267
                     /locus_tag="AC16CB_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341760.1"
                     /translation="MEYHTSSNGRKHDRSRKEIVMEEPTLLDYLKAKLTPWRKQKIEI
                     PLEEVPEEAPEKTGAGVHASQEGFSLGEELSIQGAVPSPGVARALPTSPDALQGDFLK
                     KVVLPWRSLLAFALALIAQLTMQPGPGRSWKLGVVLYLLGMAFLAWAYFRSEWAVDGS
                     PQAGEDEQFSSVSTSSWSVIRRPFFLVISFALGVISFLAFGGNNFNTLNVSLWTLGLA
                     ALVWAVWQPGEASSSLFKQIRSIFLTKEWQIRISRQGLLLLAAVLLVVFFRVYRIQQV
                     PPEMFSDHAEKLLDIWDVLHGQTSIFFMRNTGREAFQMYLTAAIIKIFGTGYSFLSLK
                     LGTVLAGLLTLPYI"
BASE COUNT          948 a         1013 c         1142 g         1164 t
ORIGIN      
        1 tcgctggatt tccactctcc atatggctgt tctaaaggta gtggggatca atacacgcgt
       61 gattattacc gcatctatgg tctgccaacc gtcgtatttc gccagagctg catttatgga
      121 ccaaggcaat ttggtattga ggatcagggc tggctggcgt ggtttatgat tgcggtagtc
      181 gcttccagac caattactat ctatggcgac ggtaaacagg tgcgcgatgt gctgtttatt
      241 gatgacctgc tggatgccta tgatgctgcc ttggagcgaa ttgatattgc cgctggtcaa
      301 gtctataaca ttggcggtgg tccggatcat gcaattgctg tgtggacgga gtttggtgcg
      361 ctgcttgaaa acctgatggg gcaaaaaatt caccctaaaa tgagtgattg gcgccctgga
      421 gaccagaaga tttacgtctc tgatatccgc aaagccgaga gagatttagc atgggctccg
      481 aaaatcggtg taaaaaaggg tctgaagcga ctctatgagt gggtgctaga aaatcgggag
      541 ttgtttagat agtatccatg cgaattctta ccgttcttac ttattaccga ccgcatacca
      601 gcggtttgac aatttatgcc gagcgattgg cacgcgcact tgccaggcgc ggccaccagg
      661 ttaccgtttt taccacccag tacgatgggt ctttagcccg agatgaaatg cagcatggcg
      721 tgcgggtcgt tcgcgcccct gtcgcattac gcattagcaa aggggtgatt gcacccgcat
      781 ttggcttcat ggcgacgcgt ttggttcaag agcatgatgt ggttcacttg catttgccac
      841 agtttgacgc accgggagta gcgctgcgcg gtcgtctcct gcgcaagccc actgttttga
      901 cttatcattg cgatcttaaa ttgccaccca atccgttcaa ctattttcta aaccggggag
      961 tgcacctggt ccatcacatg gcggcaaagc tggcaaatcg gatcgtgacc tacacccaag
     1021 attacgccga caattctcgc tacctatcca attacaaaca taagctgcaa atcataccac
     1081 cgccagtaga attacctgcg gttccgcatg gtgacgtcga ggcatttgca gaggctcaca
     1141 atacatcaga gcgccggccg gttattggta tggctgctcg cctggcaaca gaaaaagggg
     1201 tagaaattct cctggaggca ttaccgagca tcatcgaaaa atttccccgg gcacaggttc
     1261 tctttgccgg gcagtatgaa gatgtattgg gtgagaggga atactataac cgcttgatgc
     1321 cggtgatacg aggatatcag caacaaggtc agtgggagtt tttgggggtg ctgaacccca
     1381 atcaaatggc ggctttttac ccgaatctgg atgtactagt tgttccaagc ctgaattcga
     1441 ctgaagcctt tggattggtg caaatcgaag cgatgatgca tggggttcct tgtgtggcct
     1501 ccgcattacc tggcgtgcgg caacccatac ttaccaccgg tatgggcaag gtggtgccaa
     1561 ttggagattc gggcgcatta gctaacgcta tacaagctat cctggccgaa ccggatcaat
     1621 accagaagga tccgcgtgct attgcccaac gatttacacc agatgctatc gccttattgt
     1681 atgaagagat gtttgaggac atgctggcga agaaatagaa aagtttattg gtctgaatat
     1741 gcaagaaaag gattttctct ggctacacct tcgcgatctc ccatacttcc gcgctctggt
     1801 gcgggcagtt gaggctgagt attaccagga tatcgaattg ccgtccccac aattggatat
     1861 cggttgtgga gatggtcact tcgcctccct ggtttttgaa aagccagtcg atgtaggggt
     1921 tgaccctggg ggcaccgcga ttcgtcaggc tgcccgcctc ggtagttatc gctcactagt
     1981 tcaaagtgat gctggagcac tgccatttcc agaaggttac ttcggtagtg cgtttagcaa
     2041 ctcagttctt gagcatattc cgcatgtccg agacgtactg gcggagacgg cgcgtgttct
     2101 caagccaggt gcgctatttc tgttttgtgt gcccaatcca cgctatttgt cggaactttc
     2161 ggtgcctggg attttggcac gtgcccgcct gaacggctta ggtaaagcat atactgagtg
     2221 gtttcggcgc atttctcgtg tccaacatgc tcaaccccct gaagtttggt gcttatggtt
     2281 agaggctgca ggattcacag tggagaaatg gtggcattat ttttcgcccg cagctctgca
     2341 catgctggag tggggtcact actttggtgc accgagcttg cttccgcatg tgttgacagg
     2401 acgctggata ctggctcgaa cgcgttggaa tctagctttg actgaacgca ttgcgcgccc
     2461 ctatgcccag ccaaaccctc accctcaagg cacattcact ttttatataa ccaggaaggt
     2521 tgcaggctga gaataggaga gcactcgttc atgaccggtt cttcaaatcc ctacgacgat
     2581 acctatttca tgcgggcggc atatgccaat gtttcatttg cccgcttcag ccaatattgg
     2641 tggtcaaacc gctattacgc tcttcttgcc cgccgtcatg gccctccaaa tgggcgggtg
     2701 cttgaagtag gctgtggttt aggccatttg ttgggttggc tgacagatag ctatcaggtc
     2761 tttggggtgg acatcaattc gtgggcattg gcccaggcac gtcagaatgt tccccagggg
     2821 cattttgctt acctctcagc agaggatttg ggtgcttttc aggatggttc atttcagatt
     2881 gtggttgcta agcatgtggt ggagcatctg cctgatcctg atcatgcttt aaaagaaatg
     2941 agccgcgttt tggcgaagga tgggctgctt ctgctagcca cgcctaattt ggattcgcta
     3001 atgcgttctg taaaaaaaga gcgttggatc ggctatcaag atccgactca tattaatatg
     3061 aagccacccg atgaatggat tggtagcctg caccggtacg gtctatggcc taagcgggtc
     3121 ttttccgatg gtttctggga tgccccatat ataaattggc tacccactcg gctgcagaaa
     3181 ctaatctttg gagcgccggg ggggttgcag gcaatattgg gatggagtat catacctctt
     3241 cgaatgggcg aaagcatgat cgttctcgca aagaaatcgt gatggaagag cctactctac
     3301 ttgattatct taaggccaag cttactccct ggcgaaagca gaagatcgaa attccgctag
     3361 aggaagtgcc tgaagaagct ccagagaaaa caggggcagg ggtccatgcg tcccaggagg
     3421 gcttctcttt gggagaagag ttgtcgattc agggggcggt tccatcacct ggggttgcga
     3481 gagctttacc tacgtcaccg gatgcattac aaggcgattt cttaaagaaa gtggtcctcc
     3541 cttggcgctc cctgttggct tttgcacttg cgttaatcgc ccagctcacc atgcaacctg
     3601 gtccaggacg ctcatggaag ctgggcgtcg tcctgtatct gctaggcatg gccttcctgg
     3661 catgggcata ttttcgctca gagtgggcag ttgatggcag cccgcaagct ggtgaagatg
     3721 agcagttttc ctcagttagc acttcttctt ggtccgttat tcgtcgccca tttttccttg
     3781 tcatttcatt tgccctgggc gtaatatctt tcctggcctt tggtggcaac aacttcaaca
     3841 ctctgaatgt gtcactctgg acattaggct tggctgccct ggtatgggct gtttggcaac
     3901 ctggtgaagc gtcttcatcc ttatttaagc aaatacgctc catatttttg accaaagagt
     3961 ggcaaattag gataagccgg cagggtttgc ttcttctggc ggcagtattg ctagtggtct
     4021 tttttcgcgt ctatcgtatt cagcaagtcc cccctgagat gttcagtgac catgcggaga
     4081 agttattaga tatatgggat gtgcttcatg ggcagaccag cattttcttc atgcgaaata
     4141 caggccgtga agcttttcag atgtatctca ctgccgcaat catcaaaatt tttggcaccg
     4201 gctattcttt cctgagcttg aagcttggca ctgttttggc gggattactc acgctgccct
     4261 atatcta
//
LOCUS       DBNGSX010000135         7398 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_980413, whole genome shotgun sequence.
ACCESSION   DBNGSX010000135 DBNGSX010000000
VERSION     DBNGSX010000135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7398)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7398
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_980413"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="AC16CB_04495"
     CDS             complement(<1..738)
                     /locus_tag="AC16CB_04495"
                     /inference="COORDINATES: protein motif:HMM:NF026257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR3341761.1"
                     /translation="MRDNKLFHNLVPSSGPERIHDLARLISGRVGAREIPAGHWADIN
                     QLAIRHGLGPLLWCILLQQDQQVCSTAVFQPLADSVNIASFNYQLLRESQSWVQAAFS
                     QAGIPSVWFKGMALAQTHYPKPDLRPMDDQDVLVPFAQRQDALEALLALGYFFPPKDE
                     LEKRLGAPDRTAYHFHLRGGPSGAVILEVHFSLPDGSRNLPLEAVDWFLQQTDTVGQK
                     KSAFNTLKAEAHLLYLCAHTILQHGEAE"
     gene            complement(731..1624)
                     /locus_tag="AC16CB_04500"
     CDS             complement(731..1624)
                     /locus_tag="AC16CB_04500"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HIR3341762.1"
                     /translation="MDITEHIPPISCRLAGQTIWFNQSLNELQPFRLEGLTSPPCSLP
                     ANLLELVREPPLLQAVGGLADKSTSISLWRDDNGYLLEIPAAGFFWTAMSGSCIYQVF
                     RVPSANPSLLCESILGPPLALALCLSGIWCLHASAIEYENQLAVFVGSSGAGKSTLAT
                     YLADHSGIRQTADDILPVSEAVQGVNALPHFPQLKIHPEEQPGLAFPERLPLSVIYLL
                     DEGVDVSLETLSPAEAAMSLAGHTVAAHLFDRQLISQHFTFCSQSATVVPVRKLIYPR
                     KFSQLPQVWQVLKNDLAGIHA"
     gene            complement(1625..1900)
                     /locus_tag="AC16CB_04505"
     CDS             complement(1625..1900)
                     /locus_tag="AC16CB_04505"
                     /inference="COORDINATES: protein motif:HMM:NF017239.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HIR3341763.1"
                     /translation="MVNFDRRVILPDGVLMRPVGDEAVLLNLDTNTYFALNPVGADML
                     IVLTQSSSIEQAFTRLLDMYDIAPDVMRADLQELIEDLAQQGLVLIA"
     gene            complement(2415..4280)
                     /locus_tag="AC16CB_04510"
     CDS             complement(2415..4280)
                     /locus_tag="AC16CB_04510"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:NF012936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B family protein"
                     /protein_id="HIR3341764.1"
                     /translation="MSAILAIFNREAGPVDQQNVLRMLAAAPQRSIDGQQIWIQESMA
                     LAHQHFWITPEEQNEIQPLEDSGCTITCFARLDNRAELTHTLRLENHPPLTDAYLILR
                     SYLHWGVDCVEHLQGDFAFALWDSRQQTLFLARDALGIADLCYYFDQRLFLAASQVSQ
                     ILAHPGIPRELNERKVCDHLEGYSLDQENSYFENIYYLLPAHCLLVSSSAVSKWQYWD
                     VDPSLSIRYRRDEDYAEHFRELLVQAVQARLRTRGPVGISLSGGLDSTSIAAIIARLF
                     AQDPSLVGPLLSFSYVFESMPTADERQFIRPLVEHLGIEACFIPADEHWTLYDLENWP
                     VLPDTPAQDPFSWQLIANQRAASAAGCRVMYTGIFGDALYLGWRYWAADLLRDGHLRE
                     LVRLTWCERQKIALWNELFLYGLRATAPAWLKKAYLSFRPLRFPVTNPGLELDFARDT
                     QIQARRLNDYMSISKGFGAPGQWMRYQALLMNGYAESLAYQRQVANAERIEMISPYLD
                     RRLIEFILAIPAYQLDRPGTYKYILHNAMRGWLPEVVRSRTLVTSLFPLFEKGIREKA
                     QPDVLNLLNKPQIVERGYVKQSWLDQEANPGKPLTDEGYWFWLAISLELWLEKYW"
     gene            complement(4374..>7398)
                     /locus_tag="AC16CB_04515"
     CDS             complement(4374..>7398)
                     /locus_tag="AC16CB_04515"
                     /inference="COORDINATES: protein motif:HMM:NF013524.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HIR3341765.1"
                     /translation="DSNGHYNPYAVTLAAGETNTSLDFAYQAPVGRSLSGTVFEDSGS
                     SGTLGINDGSTVDPVVPGATVDLYKVVNGVEYLYATTTTDSNGSYSFTGLPEGDYVVK
                     VNTTGTDADGYTQTVDPDEPGVVCTTCDSETTTPITLSATTPSVASVDFGYWNGGVET
                     TPVSLAFFQAKRGGKNVRFEWATSTEAGNAGFNLYVQTGDDLLKINQELIPTQSSSSL
                     DWHSYNYSADVAGTTFFLEEVSVRLETRLYGPFELGQAVGKRNERQKINWGAIQSEHA
                     AKRSERAARDVAEVNRRLQELPTLEEPVNSATSDQSSSSLKLSAPTNEPTSAQLPSDA
                     LPITYFGVDQTGIHRVTYEALQAAGFDLNGIQVAQLALTNQGVPVPIRISGDKRWGAG
                     SFIEFYGQALDTIYTTTNVYTLWYDRSIAVRVAVDKTKPDPSAEIPAYYMETAQVNRQ
                     ANYFFGAPGSDPWYDTMLFVYRNNPNSWNFSLDLDEVVPGPASTLSLDLWGITQFPVS
                     PDHHAVVSFNGATVADQWFDNGEAITINATLDNINAGVNTLTVALPGDVPLGVDFDLS
                     ALDRYSVTYPRAFVARNGSLSFTSTAKVFEVAGLPSRNVVAYRIQNDEITYLSGTQVA
                     SRTGTAQFVGSANEADYIVVDEGALLSPTIQPVPADAGINSGLAQYLVIAHPDFINAD
                     LDRLVQARSAQGLSVKVVDVNQIYAQYSHGVTDPYAIQSYIKYAAANLGTQYVLLVGG
                     DTYDYHNYLGLDSISFIPSLYAATDEIVLHTPVDPLYGDTNNDELPDLAIGRFPVRTA
                     AELAMIVDKTLTYDNKSYAQTAVFAADEGFAENSDSFIAKLPAGWTSQKAYIEEVGVA
                     AAHETLIEKINEGVAMTTFVGHSDDWSWTYLGLFDTRDAAALTNYDKPTVVTQWGCWN
                     TWYVNPYYDNLGHILMLTGTNGGAAVLGATTLAYDDSELALGNLLTPKMVQPGLPIGA
                     ALQAAKAELAASQPDARDVLLGWTLLGDPYLTIQP"
BASE COUNT         1828 a         1900 c         1925 g         1745 t
ORIGIN      
        1 ctctgcctct ccatgctgca ggatggtgtg agcgcacaag tacagcagat gcgcttctgc
       61 tttaagagta ttgaaggcag attttttctg cccaactgta tcagtttgct gcaagaacca
      121 gtctactgct tccagtggta gattgcggct gccatctggc agactaaaat gaacttccag
      181 gataacagcc ccggaagggc caccgcgcag gtgaaaatgg taggctgttc gatcgggtgc
      241 ccccaacctc ttttccagtt cgtccttggg tggaaaaaaa tagccaagcg ccagaagtgc
      301 ttctagtgca tcctggcgtt gcgcaaaagg caccagcaca tcctgatcgt ccatcgggcg
      361 cagatcaggc ttcgggtaat gggtttgcgc cagagccatc cctttaaacc agaccgaggg
      421 tataccagcc tgtgagaagg cagcctgcac ccaagactgg ctttctctaa gcagctggta
      481 attaaagctg gcgatgttta cagaatcggc caagggctga aagacagcag ttgagcatac
      541 ctgctggtct tgctgtagaa gtatacacca cagcagcgga cctaatccat gtcggatagc
      601 cagctggttg atatccgccc aatggcctgc aggtatctcc cgcgccccaa cccgtccact
      661 gatcaaacgc gcaaggtcgt ggatccgttc aggaccagaa cttgggacca ggttgtggaa
      721 gagtttattg tcacgcatga atgcctgcca gatcattttt aagcacctgc cagacctgcg
      781 gcagctgtga aaatttacgg ggatagatca atttacgaac cggtacaact gtagcagact
      841 ggctgcagaa ggtgaaatgc tggctgatga gctgccggtc aaagaggtgg gctgccaccg
      901 tatgcccagc aagactcatg gcagcctcag cagggctcag tgtttcaagc gagacatcga
      961 caccctcatc cagcagataa ataactgaaa gtggcagtct ctcaggaaat gctaacccgg
     1021 gctgctcctc aggatgtatt ttaagctgag ggaaatgggg taaggcattt accccttgga
     1081 ctgcctcgga tacaggcaga atatcgtctg cggtttgccg tatcccggag tggtcggcca
     1141 gataggtcgc aagggttgac tttcccgcac cagatgaccc tacaaataca gcgagctggt
     1201 tttcatactc aattgcgctg gcatgtaagc accagattcc agacaagcat aacgccagag
     1261 ccagaggcgg ccccaagata gactcgcata agagagatgg gttcgcacta ggcacccgaa
     1321 atacctgata gatacagctt ccagacatcg ctgtccagaa gaatccggca gcagggattt
     1381 cgagtaaata gccattgtca tcccgccata gagagatgga cgtggacttg tcagctagcc
     1441 ctcctaccgc ttgaagcaac gggggttccc gaactaattc caggaggttg gccgggagag
     1501 agcagggtgg ggaggtcaat ccctccaatc ggaatggctg tagctcattc agcgattgat
     1561 taaaccaaat cgtctgtcct gccaggcgac atgaaatggg tggtatatgt tcagttatat
     1621 ccatctaggc gatcaggacc agcccttgct gggctaaatc ctcgatcagt tcttgaagat
     1681 ctgccctcat aacatccggg gcaatatcat acatgtccaa gaggcgtgtg aatgcctgct
     1741 cgatcgagga gctttgcgta agcacgatca gcatgtccgc ccccaccggg ttcaaggcaa
     1801 agtatgtatt ggtatccagg tttaacagca ctgcctcatc acccacgggc cgcatcaaaa
     1861 caccgtccgg aagaattacg cgtcgatcaa agttaaccat ggatgatttc tggcgtaccg
     1921 attgcgttca ctttagaaca atcgaacatg cgaaacactc aagtataatt aggttacaaa
     1981 tagatgggaa aagcccgaaa acctagggca atcgcatttg ggcgatttcc caccactgag
     2041 gagagaaaat ggggtgtttt ggtgggggta tgcccccacc aaaacacccc aaacactctt
     2101 tttgcgggat tgcgcatgcc cctcagggca tatgcaattg ccctgccgaa aacattattg
     2161 acagatcagg gtcctgtgcg tggtctaaaa atgaaaggag ttccgctgtc tccagtacca
     2221 ggagacccac ccagggtgtt gcttcgtata tcaccaagaa ttcttaaaac tggcctgctg
     2281 tatggttttt tatcttgagg caattttttt tgtgcagttt caaatttggt tttagtattg
     2341 taatccatag taaaattcct tactcaatat caattagtct agttaagtga tttttcattg
     2401 taggttaacc cattttacca atatttttcc aaccatagtt ccaggctgat tgccagccaa
     2461 aaccaataac cctcatccgt taatggtttt cctggatttg cttcctggtc taaccaggat
     2521 tgcttcacgt agccacgttc cacaatttgg ggtttattca agaggttgag cacatcaggc
     2581 tgggcttttt cccggatacc tttctcaaac aatgggaaaa gcgaagtgac cagcgtcctg
     2641 ctacgcacca cctcaggcaa ccagcctcgc atggcgttgt gtaggatgta tttatatgtc
     2701 ccaggtcggt cgagctgata ggcaggaata gccagaataa actcgatcaa tctccgatca
     2761 agatatgggc tgatcatttc aattcgttcg gcattagcca cctgcctctg ataagcgagg
     2821 ctctcagcgt agccattcat taataaagcc tgatagcgca tccattggcc cggagcacca
     2881 aaacccttgg atatagacat gtagtcattc agcctgcgcg cctggatttg ggtgtccctg
     2941 gcaaaatcca gttcgagtcc gggattggtg accgggaacc tgagcggtcg gaaagacagg
     3001 taagcctttt tcagccaggc tggggcggtc gctcgcaacc catacaagaa aagttcattc
     3061 cataaagcaa tcttttggcg ttcacaccag gtgagcctga caagttctct caagtgaccg
     3121 tcgcgcagca ggtcggctgc ccagtaacgc caacccagat atagcgcgtc gccaaaaata
     3181 ccagtataca tgacccggca gccagccgca ctggcggcgc gttggttagc tatcagctgc
     3241 caggagaatg gatcttgcgc gggagtatcg ggcaacacag gccagttctc caagtcgtac
     3301 aatgtccaat gttcatctgc agggatgaag caagcctcga tccccaggtg ctcgaccaaa
     3361 ggacggataa actgacgctc atcagcggtg ggcatgcttt caaaaacata tgagaagctt
     3421 aataacgggc ccaccaagct tggatcttgg gcgaataatc gggcaatgat tgcagcaatg
     3481 gaggtcgaat ccaggccgcc acttaaagag ataccaacgg gtcctcgcgt tctcagtcgc
     3541 gcttgcactg cctgaacaag cagttcacgg aaatgctccg cgtaatcttc gtcccgccga
     3601 tagcgaatgc tcagactcgg atcgacatcc caatactgcc acttggatac tgcgctgctg
     3661 gaaaccagca ggcagtgtgc tggcagcaag taatagatat tttcgaaata tgaattttcc
     3721 tggtcgaggg aatatccttc taaatgatca catactttcc tttcgttcaa ttcacgcgga
     3781 attcccgggt gagcaagaat ttggctgacc tgtgaggcag ccagaaatag tctttggtca
     3841 aagtaatagc ataggtcggc aatccccaga gcatcacgtg ccaggaatag cgtctgttgc
     3901 cggctgtccc acagggcgaa agcaaaatct ccttgaagat gctcgacaca gtcaacaccc
     3961 cagtggagat aagaacgcag gataagataa gcatcggtca aaggcggatg gttttctaat
     4021 ctcagggtat gagtcaactc tgcccgatta tccaaacgtg cgaagcaggt gatggtgcag
     4081 ccactatctt caagtggttg gatttcgttc tgttcttcgg gtgtgatcca aaaatgctgg
     4141 tgcgccaggg ccatactctc ttggatccag atttgctgtc cgtcaattga gcgctgcgga
     4201 gcagccgcta acatcctcag aacgttttgc tggtcgactg gtccagcctc ccggttaaaa
     4261 atcgcaagga ttgcactcat ttggtattat tttatatcaa gttgtgcaga aaattgaagt
     4321 ttggaatgga tataccccca ccaatagggg gtatatccat ttgcaccatg cgtttatggc
     4381 tggattgtga ggtacggatc gccaagcagt gtccaaccaa gcaagacatc cctggcatct
     4441 ggctggctgg ctgccaactc tgccttggct gcctggaggg cagcgccaat gggcagcccg
     4501 ggctgcacca tcttgggagt aagcaggttg ccaagagcca attcggaatc gtcataggcg
     4561 agcgtagtag cacccaggac agccgcacca ccgttcgtac cagtcagcat caagatgtga
     4621 cccaaattgt cataatatgg attcacgtac catgtgttcc agcaacccca ttgagtcacc
     4681 accgtgggtt tatcatagtt ggtcagcgca gctgcatccc ttgtgtcgaa gagtcccaga
     4741 tatgtccaac tccaatcatc cgaatggccg acgaaagtgg tcatggcgac gccctcattt
     4801 atcttttcga tcagagtttc atgggcagca gccacaccca cttcctcgat gtaggctttc
     4861 tgggatgtcc atcctgcagg gagcttggca ataaacgaat cgctgttttc agcaaaacct
     4921 tcgtcggcag cgaagactgc ggtctgagcg taagatttat tgtcataggt cagggtttta
     4981 tccacgatca tcgcaagttc agcagcagta cgtaccggga agcggccgat ggccaggtca
     5041 ggcaactcat cgttgttcgt gtcgccatac agcggatcaa ccggtgtatg gagcactatc
     5101 tcatcggtgg ccgcgtacag tgatgggatg aaacttatgc tgtccaggcc taggtagttg
     5161 tgatagtcgt aagtgtcgcc tccgaccagc agcacatatt gtgtgcccag attggctgcg
     5221 gcatatttga tgtagctttg aatagcatac gggtcggtaa cgccatggct gtattgggca
     5281 tagatttgat tgacatccac aacttttact gagagaccct gggcagagcg cgcctggacc
     5341 agccgatcca ggtcggcatt gataaaatca gggtgagcaa tgaccaggta ttgcgccagg
     5401 ccagagttaa ttccggcatc agctggcaca ggctgaattg ttggagaaag cagcgctccc
     5461 tcgtcaacca cgatgtaatc agcctcattc gcagaaccca caaactgggc agtaccagta
     5521 cgactggcaa cctgcgtgcc acttaagtaa gtgatctcat cattctgaat ccggtaggct
     5581 acaacgtttc tgctgggcaa cccggcgacc tcgaagacct tggcagtcga ggtaaagctc
     5641 aagctgccgt tccgagcaac aaaagcccgc gggtaagtga ctgagtagcg atccaaggct
     5701 gaaaggtcaa agtccactcc cagaggtaca tcaccgggca gggcaactgt aagggtgtta
     5761 actccggcat tgatattgtc cagggtggca ttaattgtaa tggcttcacc attatcgaac
     5821 cactgatcgg ccacagttgc tccgttgaag ctaacgacgg catgatgatc tgggcttacg
     5881 gggaattgag tgatccccca caaatcgagc gaaagagtag aagccgggcc aggaaccact
     5941 tcatccagat caaggctgaa gttccagcta ttcggattgt tgcggtaaac aaagagcatt
     6001 gtatcgtacc agggatcgct gcctggagca ccgaaaaagt agtttgcttg gcggttgacc
     6061 tgggcagttt ccatatagta agctggaatc tcagctgatg ggtcgggttt ggttttatcc
     6121 acagctaccc gcacagcaat ggaacggtca taccacaagg tgtagacgtt tgtggtggtg
     6181 taaatggtgt cgagcgcctg cccgtagaac tcgatgaaac tgccagcacc ccatctcttg
     6241 tctccactga tacgaatcgg aactgggacg ccttggttgg tcaaggccag ctgagcaacc
     6301 tggatgccat tcagatcaaa acctgccgct tgtaaagctt cgtaggttac ccggtggata
     6361 ccggtttgat ctaccccaaa gtaggtgatc ggtagggcat ctgaaggcaa ctgtgcagac
     6421 gttggttcgt tggtcggtgc tgacaacttg agactagagc ttgactgatc gcttgtggcg
     6481 ctatttacag gctcttcgag cgtcggtagc tcctgcaaac gcctgttgac ttcagccaca
     6541 tcccgagccg cgcgctcaga tcgtttcgcg gcatgctccg actgtatagc tccccagttg
     6601 atcttctggc gctcattgcg tttgcctaca gcctgaccaa gctcaaacgg accgtagagc
     6661 ctggtttcta accttacact cacttcctcc aggaagaaag tcgttcctgc cacatcggca
     6721 gaatagttgt aggaatgcca atccagagag gaagagctct gggttgggat taactcctgg
     6781 ttgattttca gcaagtcgtc cccggtttga acgtacagat tgaagccggc gtttcctgcc
     6841 tcggttgacg tagcccactc aaatcgtacg tttttgcctc cacgcttggc ctggaaaaag
     6901 gccagggata caggcgtggt ctcaacaccg ccattccagt agccaaagtc gacgctggcg
     6961 acggatggtg ttgtagcaga aagcgtgata ggcgtcgtcg tctcgctgtc gcaagttgtg
     7021 cataccacac cgggttcgtc agggtcgacc gtctgcgtat aaccgtcggc atcggtcccc
     7081 gtcgtattaa ccttgacgac ataatctccc tcaggcaatc ctgtaaagct gtagctgcca
     7141 ttggaatctg tcgtcgtggt agcatagaga tactctaccc cattcaccac tttataaagg
     7201 tccaccgtcg cacccggtac gaccgggtca accgtcgaac cgtcattgat gcccagggtg
     7261 ccagaactgc cactgtcctc aaacaccgtg ccgctcagcg agcggcctac aggtgcctgg
     7321 taagcgaagt caaggcttgt gttggtctct ccggctgcca gggttactgc gtaggggttg
     7381 tagtgaccat tgctgtct
//
LOCUS       DBNGSX010000136         2153 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1395634, whole genome shotgun sequence.
ACCESSION   DBNGSX010000136 DBNGSX010000000
VERSION     DBNGSX010000136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1395634"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2153
                     /gene="glpB"
                     /locus_tag="AC16CB_04520"
     CDS             <1..>2153
                     /gene="glpB"
                     /locus_tag="AC16CB_04520"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HIR3341766.1"
                     /translation="DVKISDPDHFAIEPWEVHLCLEEGDKLVPGFKNMRMLRAWAGVR
                     PLYQESQVSDTRDVTRAFVLLDHELRDGVTGIVTITSGKWTTYRKMAEVTVDLVCKKL
                     GTQRACRTHLEMLPGPDHKKHYLGARLAQIEQDKSYGQLVCECELATYADVTRSILER
                     NAKTIDDVRRDVRLGMGPCQGGFCTYRVAGILHRLAPSQVDSSKPSVELTNVALRDFL
                     QERWKGLLPVLWGQQLRQERLDELIYLDVLNADHLPGPMSSRLGPEMYAQPSKLEGNS
                     AAADMFLPEQSQTPTSRTPAPITKREGVRKRSLLDILVIGGGLAGLFSAWKVAQKGKK
                     VRLVTKGWGALHWGSGCINVLGYYPLDSTDPLQSPVAGIKQILRENSKHPYALVQLEH
                     IEAALEDFKTLSAQLGYPFHGDLDRNWLLPSALGTFRPACLVPETMVAGDLRQNQPML
                     IVGFEQYPDFYSELIAANLTRQGRQALGVTIDLPSLRQQRFVSGRVLASLFESAEFRR
                     EVAWQLKTHLQGAKRVGFPAVLGLKDTLKVKNELETLLEMPVFEIPTLPPSIPGIRLH
                     NLLIAEIERLGGQVYDGMQAQSAAYDGARILTVFTEAAARLKPHHANTFILATGGILG
                     GGIHFEYGQKPSEIVFNLPVTAPDDHNNWFESQFLATTSHPIYQSGILVSDEFKPMQA
                     DGSPLFENLYIAGNQIGSCDSIRERSAEGIALATA"
BASE COUNT          524 a          564 c          565 g          500 t
ORIGIN      
        1 gatgtaaaaa tctccgatcc agaccacttt gccatagagc catgggaagt gcatctctgc
       61 ctggaagaag gtgacaaact ggtacccggc tttaagaata tgcgcatgct gcgtgcctgg
      121 gcgggggtcc gtccactcta ccaggagagc caggtcagcg atacacgcga cgttacgcgt
      181 gcttttgttc tcctcgacca tgaactgcgc gacggagtaa cgggtatcgt caccatcacc
      241 agcggcaagt ggaccacgta ccgcaagatg gctgaggtca ctgtcgatct ggtgtgcaag
      301 aaattaggga cgcagcgtgc ctgccgcacc catttggaga tgctccctgg accagatcac
      361 aagaaacact atctgggcgc ccgcctggcg cagattgagc aagataaaag ctacggtcaa
      421 ttagtctgcg aatgcgagct tgccacgtac gcagatgtaa cccgatctat tctcgagcga
      481 aatgcgaaaa ccatcgatga tgtgcgccgt gatgtgcgcc tgggcatggg accctgccag
      541 gggggctttt gtacctaccg cgtggcgggt attctccacc gtttggcacc atcacaagta
      601 gatagctcaa aacctagcgt agagctaacc aatgtcgccc tgcgcgattt tctccaggag
      661 cgatggaagg gtttgctgcc cgtgctatgg ggccagcaac ttaggcagga acgcctggat
      721 gaactgatct atttggatgt gctgaatgcc gatcacctgc ctggtcctat gagctcacgc
      781 ttggggccag agatgtatgc tcaaccttcg aaactggagg gaaatagcgc agctgcagat
      841 atgttcctac cggaacaatc tcaaacccca acctcccgaa ctcctgcgcc gattacaaag
      901 cgtgaagggg tgagaaagag atcattactg gatatccttg tcattggagg tggtttggct
      961 gggctattct ctgcctggaa agtagcgcag aagggtaaaa aagtgcgcct ggttacaaaa
     1021 ggctggggtg cgcttcactg gggctctggg tgcattaacg tattaggtta ctacccacta
     1081 gatagcacag atcctttaca atcacctgta gcggggataa agcaaattct ccgcgaaaat
     1141 tcaaaacacc catacgcatt ggtccaactg gagcatattg aagctgcgct ggaagacttt
     1201 aaaactttgt ctgctcagtt gggctatcca tttcacggag atttggacag aaactggcta
     1261 ctcccctcgg cgctggggac attccgtccc gcttgcctgg tgcctgagac tatggtggcc
     1321 ggcgacctac gccagaacca accgatgctc atcgtgggat tcgagcagta tcctgacttc
     1381 tattccgagc tgatcgccgc aaacctcact cgacaaggta ggcaagcttt aggggtgaca
     1441 attgatttac cgtctctgcg ccagcagcgc ttcgtcagcg gtcgggtatt agcatcactg
     1501 tttgagtcag ccgagtttcg tcgtgaggtc gcttggcaat taaaaacaca tttgcagggc
     1561 gcaaaaaggg tgggtttccc ggctgtcttg ggcctgaaag acactcttaa agtcaaaaat
     1621 gaactcgaaa cgcttctcga aatgccggtt tttgagatcc ccaccctgcc accctccatc
     1681 cctgggatcc gtttgcacaa cctgttgatt gcagagattg agcggctcgg cgggcaggta
     1741 tacgatggta tgcaagccca atcggctgct tatgatggag cacgaatatt aaccgttttt
     1801 acggaagctg cagcgcgctt aaagccccat catgccaaca ccttcatcct tgctaccgga
     1861 ggtatcttgg gtgggggaat ccattttgaa tacgggcaaa aacccagtga aattgtgttc
     1921 aatttgcccg ttacggcacc agacgatcac aacaattggt ttgaatccca gttccttgct
     1981 acaacaagcc atcctattta ccaatcaggc atattggtga gcgatgaatt taaacccatg
     2041 caagccgatg gcagccctct cttcgaaaat ctctatatcg ctggcaacca aattggttcc
     2101 tgtgattcca tacgcgagcg ttccgccgaa ggcatcgcct tagccactgc ata
//
LOCUS       DBNGSX010000137         2715 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_772839, whole genome shotgun sequence.
ACCESSION   DBNGSX010000137 DBNGSX010000000
VERSION     DBNGSX010000137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_772839"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..616
                     /locus_tag="AC16CB_04525"
     CDS             110..616
                     /locus_tag="AC16CB_04525"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR3341767.1"
                     /translation="MSEVTEINGINVAELLEFKNICKQDPSKADRDPVLVAHWVGGSR
                     SRVEFKDKVIHLGGDDEFNPMQMFLATLAACDVDLVAMHASFLGIKIESLSVEVTGHF
                     NVRSYLGLEGAPGPGYDSISYTVRLSAPKITPEQVAQLRERCDRSSPVGDSMARVIPL
                     KLEFTANT"
     gene            715..1533
                     /locus_tag="AC16CB_04530"
     CDS             715..1533
                     /locus_tag="AC16CB_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001986571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3341768.1"
                     /translation="MMDNYICITCGHQFAESVGPPERCPICEDDRQFVHPRGQSWTTV
                     DKLAREHRNVLVALEKGLTSINTQPKFAIGQRSLLVQTSRGNVLWDCVSLIDEPTIEA
                     VGHLGGISALAMSHPHLAGSMVEWSRAFGDAPIYLHADLEPWIQRPDPVIQFWEGEDL
                     LLEEGLSLHRCGGHFEGSTVLHWPEGADGQGVLLTGDTMYVTPDRKHVSFMYSFPNFI
                     PLPAPVVERIVERALTLRFDRMYGHFADLDIKADAKQVVKRSAERYKRAIGVED"
     gene            1612..2064
                     /locus_tag="AC16CB_04535"
     CDS             1612..2064
                     /locus_tag="AC16CB_04535"
                     /inference="COORDINATES: protein motif:HMM:NF019544.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol phosphotransferase CPT family
                     protein"
                     /protein_id="HIR3341769.1"
                     /translation="MPRRYLDRPLWDEVLGLATEAGPVGQRLISGMHQSIAALSRAGV
                     NVVADHVLVEQEWLQECVILLSVLPALFVGVRCPLAVLEAREAARKDRTLGQARAQFD
                     LVHEHGIYDLEVDTSQLSAQACAQQIKERIEEDRSPDAFKRLRQEMAA"
     gene            2212..2646
                     /locus_tag="AC16CB_04540"
     CDS             2212..2646
                     /locus_tag="AC16CB_04540"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3341770.1"
                     /translation="MITIERSIFINRPPEDVFDFMSDPTNDMKWRDSAVSAEWTSEAP
                     HGVGSTLRSVDKILGREMESAAEITAWDRPNLWGQKSVGGPMPFEMTVKFEAKGQGTQ
                     LTLRGSAEPGGFFKLAEGLVRKQLLNQFDTDLNGLKRVMEAG"
BASE COUNT          652 a          628 c          778 g          657 t
ORIGIN      
        1 gatttatatc cgaattaacc gaatagttgc gttatataca ctttttgggc aacctgcttc
       61 cgccaatagt attatcaagt caaagcatgt ttgatagaaa ggagaaaaaa tgagcgaagt
      121 aactgaaatc aatggcataa acgttgctga attgctggag ttcaagaata tatgcaagca
      181 agatcccagc aaggcagacc gcgatcctgt attagttgcg cattgggttg gcggctcacg
      241 ttcgcgagtg gaatttaaag ataaagttat ccacctcggc ggcgacgatg aattcaaccc
      301 aatgcaaatg tttcttgcaa cacttgccgc ttgtgatgtt gatttggttg caatgcacgc
      361 ttcctttctc ggtataaaaa tcgagagtct ctcggttgaa gtgactggtc attttaatgt
      421 acggtcatac ttaggtttag aaggtgcgcc aggccctgga tacgatagca tttcctacac
      481 cgtgcgcctt tccgctccca aaataacccc tgaacaagtt gctcaattac gagagcggtg
      541 tgaccgttcg tcacctgtgg gtgattcgat ggcacgggtg atacctttaa aattagaatt
      601 tacagcgaat acttgacaag gcatttgatt ggtaagggtc tgttcctttg tcgatctaaa
      661 tctgtcggtt gtaagaattt ctcaaacagc cagtattgcg atcgagagta gaacatgatg
      721 gacaactaca tctgcatcac atgcgggcat caattcgccg agagcgtggg acccccggag
      781 cgctgtccaa tctgtgaaga tgatcggcag ttcgtacatc ctcgcggtca gtcctggact
      841 acagtagata aacttgcccg agagcatcgc aatgtattgg tggctctaga gaagggcctg
      901 accagcatta atacgcagcc gaagttcgcg atcggccagc gctctttgct tgtgcagaca
      961 tccagaggta atgtcctgtg ggattgtgtg agccttattg atgagcccac tattgaggct
     1021 gtaggccatc tgggcgggat ttcagctctg gcgatgtccc atccccactt ggcgggaagc
     1081 atggtcgagt ggagtcgtgc attcggcgat gctcccattt acttgcatgc cgaccttgag
     1141 ccttggattc agcgaccgga tcccgtcatc caattctggg agggcgagga tttgctactg
     1201 gaagaagggc tctcgctcca tcgatgtggc ggccatttcg aaggctcgac ggtcctgcat
     1261 tggcctgaag gggcagatgg ccaaggtgtg ttgttgaccg gtgacacaat gtatgtgacc
     1321 cccgatcgca agcacgtttc gttcatgtac agtttcccta atttcattcc tctcccggca
     1381 ccggttgttg aaaggatcgt tgagcgggct ttgaccttga gattcgaccg gatgtacggt
     1441 cacttcgccg acctagatat caaggcggac gccaagcaag tcgtgaaaag atcggcggag
     1501 cgatacaaac gagcaattgg ggtcgaggat tgagactgta cccccgatgc tgcctagccg
     1561 ctcgctggag cggatgaagg ctcagcgcgg tttcatgtac gaggtaggcc catgccccgg
     1621 cgttatcttg accgcccgtt gtgggatgag gtattgggtt tggcgacgga agcaggtccg
     1681 gtggggcaga gattgatatc cggcatgcac cagtccattg cggcattatc cagagccggc
     1741 gttaatgtgg tggcagacca cgtgttggtg gagcaggagt ggttgcagga gtgcgtgata
     1801 ctattaagtg tattgccagc cctttttgtt ggtgttcgat gtcccttggc tgtactagag
     1861 gcgcgtgagg cagcgcgaaa agatcgcacg ttagggcagg cacgggcgca gtttgatctg
     1921 gtacatgaac atggcattta tgatcttgaa gtggatacat cgcaacttag cgcccaagct
     1981 tgtgcgcagc aaatcaagga gcgtatcgaa gaggatcgat caccagatgc tttcaagcgc
     2041 ttgaggcagg agatggcggc ttaaccactc tcctgaagag tttgcccaca ttggagaaga
     2101 ggcgcgctgc ccagaaggtc gatccaagga ggaaagtcca tgagccaatg atcttcatcg
     2161 catttttttg tttcagtatc cggtttctat tagccaaaag gagaacagac catgatcaca
     2221 attgaaagaa gtatcttcat caaccgtccc ccagaagatg tcttcgactt tatgagcgat
     2281 cccactaacg atatgaaatg gcgagacagc gcggtttcgg cggaatggac ttccgaggcc
     2341 ccgcacgggg tcggctcgac cctgcgctcg gtggacaaga tattggggcg cgaaatggaa
     2401 tccgccgcgg agatcaccgc ttgggatcgc cccaatcttt gggggcagaa atcggttggc
     2461 ggaccgatgc cgtttgagat gacagtcaag tttgaagcta aggggcaagg cactcagctg
     2521 acattgaggg gcagtgcgga gccgggtggc ttcttcaagc tagcagaagg gctggttcgt
     2581 aagcaacttc taaaccagtt tgacaccgat ttgaacggtc tgaagcgggt gatggaggca
     2641 ggctaggcag gcctcccgag agcacatagt cccaatgatt gaagatggcc agctgactca
     2701 cagatgaaaa gctgg
//
LOCUS       DBNGSX010000138         8111 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_564642, whole genome shotgun sequence.
ACCESSION   DBNGSX010000138 DBNGSX010000000
VERSION     DBNGSX010000138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8111)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8111)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8111
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_564642"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="AC16CB_04545"
     CDS             <1..695
                     /locus_tag="AC16CB_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048039 - ubiquinone binding [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="complex I subunit 4 family protein"
                     /protein_id="HIR3341771.1"
                     /translation="VLPLYPVEAKYYAGALGFLAVMAIIFGAFASYGQDDFKRLVAYS
                     SVNHMGFVVLGIAAAAFASGTQDADIALNGAVLQMFNHGLSAAGMFFLVGVIYERTHT
                     RNLKDFGGLFPLVPVYGGILIFTAMGSLGLPGLGGFISEFLVVRGSWPIFTLYTALAM
                     LGLLFTGAYILKGVKMVLHGPLNEHWVGHLTEINAREIFVIAPLMVLMLWIGVWPGWI
                     LDVINKAVVMLF"
     gene            751..2223
                     /locus_tag="AC16CB_04550"
     CDS             751..2223
                     /locus_tag="AC16CB_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048039 - ubiquinone binding [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 4 family protein"
                     /protein_id="HIR3341772.1"
                     /translation="MQFPILSVIVFTPILAGVLILLIPAERKNEVRVTALAAATFALF
                     LSVWAYFAYDISAGGYQFVEKYTWLPALGISYHVGIDGMNAPLVLLTGVVMFTGVLIS
                     WGIDDRPREFFAFLFILATGVFGVFVALDLFMLFFFYEIAVFPMYLLISIWGWKETRE
                     YAAMKLTLYLFIGSVISLVGALAMYFTSGLNTFDMLELEKAGFSLAFQQLWFPFVFFG
                     FAVLGGIFPFHNWSPDGHVAAPTAVSMFHAGVLMKLGAFAALRVGIMLLPEGAKFHLP
                     WIILLSLVNVVYGAYIAMVQTDFKYVIGFSSVSHMGLVAMGFATLTRNGMLGAGVQMF
                     SHGVMTALFFAVVGMVYDQAHTRDIPRLGGFRNVMPLVAVAFIIGGLVSMGMPGFSGF
                     IAEFPIFMGVWESHPWIAIVAAISIVITASYIIRIIGKVFFGPMPEEFGDHMHDVTVL
                     DKVALVFLSAILVGLGVYPSVMVPLVESGVTNILRLLGGA"
     gene            2224..3681
                     /locus_tag="AC16CB_04555"
     CDS             2224..3681
                     /locus_tag="AC16CB_04555"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HIR3341773.1"
                     /translation="MSSVSFEPSMILAILPEILILVLAILVLTLDLVLPEERRGSLGW
                     ITAGGLFAIIGVSLAVGQPAADAKLVWGGMLRHDWLGFVFKMTFLFAAAITAMFSTDL
                     ARMGKRGEYYLLMLSSTLGMTLMASAADLIMLYLAIETTSIPLYILAGFLTRDDKSTE
                     SGFKYLLFGAMTSSVMVYGFSLLYGFAGTTNLYALAEGVMAGDLPIVSLFGSLLLVLV
                     GFGFKISAVPLHFWAPDTYEGAPTPITGFLSTASKAAGFAVLMRVLIAAFPPEITPLW
                     AAVIAALSTATMTLGNTLALAQRNIKRMLAYSSVAHAGYALIGLVAVSGLGIASVVFY
                     LVVYVVTNLAAFGVVAAFGKVTGSDEIAAYAGLSRRSPWLALTFMVALLSLSGMPPFG
                     GFVVKFAVFAAAVQAGQVWLAVVGVLNSIVGLYYYLTVLKVVYLYRSDEEDKPFPISR
                     SYAVALTVLVFGIILLGTVFAPWFSVSNSAAAALF"
     gene            3800..4096
                     /locus_tag="AC16CB_04560"
     CDS             3800..4096
                     /locus_tag="AC16CB_04560"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HIR3341774.1"
                     /translation="MESTPKQPERSRSRYAINMTLAAVAGQVGCLTLLIVLAALFGGL
                     WLDMRFGTKPMITVVLMVASVPVTLAGMFWIVRMAVSRINSASPANSTPREGEN"
     gene            4099..5085
                     /gene="atpB"
                     /locus_tag="AC16CB_04565"
     CDS             4099..5085
                     /gene="atpB"
                     /locus_tag="AC16CB_04565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR3341775.1"
                     /translation="MEQKKNRFFGIPNRYFVLAFIVLGYFAARAFPPILPHIQLPAER
                     VSHDPLFTLPVIGDFYWTNTLWAMLLVDVILIIIALVVRRAASGGSLVPSGFSGAVEA
                     LLEVIYNLTESTSGKWAKSILPFFVTITLMVLVANWIELIPGVDSIGVLEESDHGYAV
                     QEVLPGVATIIKGEEEVEHGGYSLVPYVRVLSTDLNFTVGLALISVIMTQVLGVRSLG
                     GKYFTKFINVGGLFSRPVFGVIDFGVGLLELVSEISKILSFSFRLFGNIFAGSVLLFV
                     IGTLVPVFAQSLFLMLEFFVGLIQAFVFGMLTMVFMAQATAGHGDHGDEQGH"
     gene            5130..5357
                     /gene="atpE"
                     /locus_tag="AC16CB_04570"
     CDS             5130..5357
                     /gene="atpE"
                     /locus_tag="AC16CB_04570"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIR3341776.1"
                     /translation="MEVAAAKLIGAGLAMIGTIGAGLGIGVLVNGAVQGIARNPDASA
                     NVQTNMILGIAFAEAVAIYCLVVALLILFVG"
     gene            5384..6121
                     /gene="atpF"
                     /locus_tag="AC16CB_04575"
     CDS             5384..6121
                     /gene="atpF"
                     /locus_tag="AC16CB_04575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR3341777.1"
                     /translation="MVALGLNLGYLLVQVFNFLIIFIVLRAWVVDPIVNILERRRETI
                     AQGLEDARVAAEARANAEVEAREIVAKAQTEANQKVREATERAEAAAREVQARADADA
                     AKAREAALAEAALERDRILADVRGQIAALAMAAAQRLIGDALDESRQRVLIEEFFSGV
                     RTGKVVVLEGEGISGSSAEITSALPLTPAEQEAVKSDVLSKIGSQSTVTFRVDPSILG
                     GLVVRVGDKVLDGSVSGQLETLRQSLR"
     gene            6214..7785
                     /locus_tag="AC16CB_04580"
     CDS             6214..7785
                     /locus_tag="AC16CB_04580"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HIR3341778.1"
                     /translation="MGRTVAWNHLVQNLPPELGKAALKAQPLPAMVTGIALDSRQVRP
                     GNVFVALTGGSTDGHGYIPEAVQRGAVAIVGEKPMGGLAVPYLQVAHSREALACLAAA
                     FYDLPARRLTVIGVTGTDGKTTTTNLIYRIMLDAGLRAGMISTVNAVVGDQELDTGFH
                     VTTPEAPDVQNYLAQMVEAGITHVVLEATSHGLAQGRVTACDFDLGVVTNITHEHLDY
                     HGTYEAYREAKGRLIASLADTPSKAQGNYRLAVLNRDDSSHDYLAQLVQQRISYGMHP
                     QADLRGVELEERAGGLRFVAKGAGMRLAVSSPLLGEYNVSNCLAAIGATVSGLGLDPQ
                     AAQAAIANFTGISGRMERIDLGQDFLAIVDFAHTPNALKVALSAARKMVGRRGRVIAV
                     FGSAGLRDRMKRRMMAEISVELADLTILTAEDPRTESLDKILAEMAAGAAGRGGVEGH
                     TFWRVPDRGEAIRHALRIAKAGDVVIALGKGHEQSMCFGETEYKWDDRIAMRAALSEL
                     LAIRGPSMPYLPTQR"
BASE COUNT         1522 a         2062 c         2439 g         2088 t
ORIGIN      
        1 tggtgctgcc gctttatccc gttgaggcga agtactatgc cggagcgctg ggtttcctgg
       61 cggtgatggc gattatcttt ggggcttttg cctcctacgg gcaagatgat ttcaagcggc
      121 tggttgcgta ttcttcggtc aaccacatgg gatttgtggt gttaggtatc gctgcggcag
      181 cctttgccag cggcacacaa gacgctgata ttgcgctcaa tggagctgtg ctgcagatgt
      241 tcaaccacgg gctctccgca gcgggaatgt tctttttggt gggtgtgatc tatgagcgca
      301 cgcacacgcg caacttaaag gactttggcg ggcttttccc actggtgccc gtatatggtg
      361 gcatcttgat cttcaccgcc atgggatcgt tgggattgcc cgggcttggc ggctttatct
      421 ctgagtttct ggtcgtgcgg ggatcctggc ctatcttcac cctgtatacc gccctggcga
      481 tgcttggatt gctattcaca ggcgcttata tcctcaaggg tgtcaagatg gtgctgcatg
      541 gcccgctgaa cgagcattgg gtgggtcatc tgaccgagat caacgctcgc gaaatctttg
      601 tcatcgctcc actgatggtt ttgatgctgt ggataggcgt ctggcccggc tggatactgg
      661 acgtgatcaa taaagctgta gtgatgctct tctaatgtgc gaagtgcgac gcgccattga
      721 tgaggccctt cgcacaggat gaggtgattt atgcaatttc cgatattgag tgtgatcgtc
      781 tttaccccaa tcttagctgg tgtgcttatc ttgctgatac cagctgagcg taagaacgag
      841 gtgcgtgtta ccgccctggc tgcggcgact tttgcattgt tcctttctgt ctgggcgtac
      901 ttcgcatacg atatctctgc tgggggttat caatttgtgg aaaaatacac ctggttgccg
      961 gccttgggga tatcctatca cgtgggtatc gacgggatga atgccccact ggtgctgctg
     1021 acaggcgtgg tcatgttcac aggcgtgctc atttcgtggg gtattgatga ccggccgcgc
     1081 gaatttttcg ccttcttgtt tatcctggca accggtgtgt ttggcgtctt cgtcgccctg
     1141 gacctgttca tgctgttctt cttctatgag attgccgtct tccccatgta cttgctgatc
     1201 tccatttggg gctggaagga gacgcgcgaa tacgccgcca tgaagctgac cctttacctg
     1261 tttataggtt cggtgatctc gctggttggt gccctggcga tgtatttcac ctccgggttg
     1321 aacacttttg atatgctcga gctggagaag gctggttttt ccctcgcttt ccagcaacta
     1381 tggtttccat ttgtcttttt cgggtttgcg gttctgggtg gcatcttccc cttccacaat
     1441 tggagcccag atgggcacgt ggccgcgccg acggcggttt cgatgttcca cgcgggtgta
     1501 ttgatgaagc taggcgcttt tgccgccttg agggtgggta ttatgctcct gcccgaaggc
     1561 gctaagttcc acctgccatg gataatcctg ctctcgctgg tcaatgtcgt ctacggcgct
     1621 tatattgcca tggtgcaaac cgatttcaag tatgtcattg gcttctcttc tgtctcccat
     1681 atggggctgg tggcgatggg tttcgccacg ctgacgcgca atggcatgct gggcgcgggc
     1741 gtgcagatgt tctcgcatgg tgtgatgacg gctctcttct ttgctgtggt cggcatggtg
     1801 tatgaccagg cacacacgcg agatatccca cgcctgggtg gcttccgcaa tgtgatgcct
     1861 ttggtggctg tggcatttat catcggcggt ttggtctcga tggggatgcc tggcttttcc
     1921 ggtttcattg ccgaattccc gatttttatg ggcgtgtggg agagccatcc ttggatcgcc
     1981 attgtagcgg ccatatcaat tgtgataacc gcttcgtata tcatccgtat catcggcaag
     2041 gtgttttttg gcccaatgcc ggaagagttt ggagatcata tgcatgatgt cactgtgctg
     2101 gacaaagtgg cgctggtctt cctttcggcg atcctggtcg gcctgggtgt gtatccatcg
     2161 gtgatggtgc cactggtcga aagcggggtg accaatatct tgcggctgtt gggaggtgca
     2221 taggtgtctt cggtgagttt tgagccttcc atgatcctgg caatactgcc ggaaatcctg
     2281 attttagtcc tggctatcct tgtattgacg ttggacctcg tcttgcctga ggaacgccgc
     2341 ggcagcctgg gttggattac tgcgggtgga ctctttgcta tcatcggcgt gagtctggct
     2401 gttgggcaac ccgctgccga cgccaaactt gtttggggtg gcatgttgcg gcacgactgg
     2461 ttgggattcg tgttcaagat gaccttctta ttcgcagccg cgatcaccgc aatgttctcg
     2521 acggacctgg cgcgcatggg caaacgcggc gagtactacc tgttgatgct gtcttccaca
     2581 ttgggtatga ccttgatggc atctgccgcg gacctgatca tgctctacct ggcgatcgag
     2641 acgacatcca tcccgctcta tatcctggca ggtttcctga cccgcgatga caagtccact
     2701 gaatcgggct tcaagtacct gctgtttggc gccatgactt cttccgtgat ggtgtacggc
     2761 ttcagcctgt tgtacggttt cgccgggaca accaacctgt atgccctggc tgagggcgtg
     2821 atggcgggag atctgccaat tgtgtccttg tttggcagcc tgctgctggt gctggttggt
     2881 tttggcttca agatatcggc agtcccgttg catttctggg caccagatac ctacgaaggc
     2941 gcgccgaccc cgatcacggg tttcctgtca actgcttcga aagccgctgg ctttgcagtg
     3001 ttgatgcgcg tcctgatcgc agcctttccc cccgagatca cacccttgtg ggctgcggtc
     3061 atcgctgcac tttccactgc caccatgacg ctcgggaata cacttgccct ggcgcagcgc
     3121 aatattaagc gcatgctggc ttattcatcc gtagcccatg ccggttatgc cctgatcggg
     3181 ctggtggctg tctctgggct gggaattgcc agcgttgtct tttacctggt ggtgtacgtc
     3241 gtcactaacc tggctgcctt cggcgtggtg gcagcatttg gaaaagtgac tggctcagat
     3301 gagatcgctg cgtatgccgg gttgagccgc cgatcgccat ggcttgccct gacattcatg
     3361 gtggcattgc tttcgctatc aggcatgccg ccattcggcg ggttcgtagt gaagtttgcc
     3421 gtcttcgcgg ccgcggtgca ggcaggacag gtttggctgg cggttgtcgg cgtattgaac
     3481 tcgattgtcg ggttgtacta ctacttgacg gtgcttaaag tggtgtacct gtatcgctcc
     3541 gatgaggaag ataagccgtt tccaatctcg cggtcgtatg cggtggcttt gacggtgctt
     3601 gtctttggaa tcatcctgct cggcacagtt tttgcgccct ggtttagcgt gtccaactcg
     3661 gccgcggcag cgctctttta aaagatagat gatcattttg gaatcacatg ctgcggatta
     3721 accgctcagt atgaataatt gatcagaatt atcatatttg ttgacgatcc gcaaccgcat
     3781 gtgataaaat tcgctaacta tggagtcgac cccgaaacag cccgagagaa gccgctcccg
     3841 ttatgcaatc aatatgacct tggcagcggt ggctgggcaa gtgggctgcc taaccctgtt
     3901 gatcgtgtta gcagcccttt ttggtggttt atggctggac atgcgttttg gcaccaaacc
     3961 gatgatcacc gtggtgctca tggtcgccag cgtcccagtc actttagcag gaatgttttg
     4021 gatcgttaga atggcggtct cccgcatcaa ttcggcttct ccagcaaact ctacgccgcg
     4081 tgaaggagaa aattgacagt ggaacagaaa aagaacagat ttttcggaat tccaaatcgc
     4141 tattttgtgc tggcgtttat cgtcctgggt tatttcgcag cccgggcatt cccacccatc
     4201 ttgccacaca tccaactacc cgcagagcgc gtctcgcatg atccgctctt tacattgccc
     4261 gtaattggcg acttttactg gacgaatacg ctgtgggcta tgctcttggt ggatgtgatc
     4321 ttgatcatca tcgcgctggt cgtgcgcagg gctgccagcg gaggcagcct ggtgccgagt
     4381 ggtttcagcg gtgcagtgga agccctgctt gaggtgattt ataacctcac cgaatctaca
     4441 tccggaaaat gggccaagtc catcctgccc ttctttgtca ccatcacctt gatggtgctg
     4501 gttgcgaact ggattgagct catcccaggc gtggatagca tcggagtcct ggaggaatcc
     4561 gatcatggct acgcagtgca ggaggtcttg cctggggttg ccacaattat caagggtgaa
     4621 gaagaggtcg agcacggcgg ttattcatta gtcccctacg tgcgcgtgct ctccaccgac
     4681 ctgaatttca cggtaggcct ggcgctgatc tcagtgatca tgacccaggt gctgggtgtg
     4741 cgctctttag gcggcaagta cttcaccaaa tttattaatg tcggcgggct gttctcgcgg
     4801 ccggttttcg gggtgatcga ctttggcgtc ggtctgctgg agctggtctc ggaaatctca
     4861 aaaatccttt cgttctcctt ccgtcttttt ggaaatatct tcgctggctc ggtgctgctg
     4921 tttgtgatcg gcacgctggt gcctgtattc gcccagtctc tctttttgat gctggagttt
     4981 ttcgtaggcc tgattcaggc ctttgtgttt ggtatgctca ccatggtgtt catggcccag
     5041 gcgaccgccg ggcatggcga tcatggtgat gagcaaggtc attaaatagt caatacttta
     5101 tctaaagttt caaggaggat cgattaacaa tggaagttgc agctgcaaaa ctcatcggag
     5161 caggtttggc aatgattgga acaataggtg cagggttggg tatcggcgtg ctggtgaatg
     5221 gcgccgtgca gggcatcgcc cgcaacccgg acgcatctgc aaatgtccag actaacatga
     5281 tcttggggat tgccttcgcc gaggccgtag ctatttactg cctggtagtc gccctgttga
     5341 tcttatttgt tggctaaatc caagctgtga aaggagataa gccttggtag cattaggtct
     5401 taacctggga tacctgttag ttcaggtttt taacttcttg attattttta tcgtcctgcg
     5461 agcgtgggtg gtcgacccga tcgttaacat actggagcgc cgccgcgaga caatcgctca
     5521 aggtctggaa gatgcccgcg tggctgccga agcgcgagcc aatgccgagg tcgaagcgcg
     5581 cgaaattgtt gcaaaagctc agactgaagc caaccagaag gtgcgagagg cgacagagcg
     5641 ggctgaagct gccgcacggg aggtgcaggc gcgcgcggat gccgacgcag ccaaagcccg
     5701 tgaagccgct ctggcggaag ccgcattgga acgcgaccgc atactggccg atgtgcgcgg
     5761 gcaaatcgcc gctctggcga tggctgctgc ccagaggctg attggcgatg ctttggatga
     5821 gagccggcag cgcgtcctga tcgaggagtt cttctcggga gtgcgcaccg ggaaggtggt
     5881 ggtattggaa ggcgagggca taagcggctc gtctgccgag attaccagcg ccctgccgct
     5941 cactcctgcc gagcaggaag cggtgaaatc tgatgtcttg agcaagatcg gttcccagtc
     6001 cacggtgacc ttccgcgtcg acccgagcat tttgggtggc ctggtggtgc gcgtgggcga
     6061 taaggttctg gatggctcag tgagcggtca gctagagacc ctccgccaga gtttacgcta
     6121 gtttattctt agaaaagccg ccaaggcgca aaggacgcga tggaggttgg ccatcctcgg
     6181 cgtgctttgc gccttggcag ttaaaataaa cccatgggtc gcaccgttgc gtggaatcat
     6241 ttggtccaga atctgccccc tgagctgggc aaggcggccc tcaaagcgca acctttgcca
     6301 gctatggtga cgggcattgc cctggattcg cgccaggtgc ggccgggtaa tgtctttgtc
     6361 gcactcaccg gcggcagcac cgacggtcat ggttatatcc cggaggcagt gcagcgcggg
     6421 gcagtcgcca tcgtggggga gaagccgatg ggaggacttg cggtccccta cctgcaagtt
     6481 gcgcatagcc gtgaagcgct tgcctgcctg gcggcggcgt tttatgactt gccggcccgt
     6541 cgcttgacgg ttatcggcgt gaccggcacg gatggcaaga ccaccacgac gaatttgatc
     6601 taccgcatca tgctggatgc aggcctccgg gcggggatga tctctacggt caatgccgtc
     6661 gttggagatc aggagctgga tacgggattc cacgtcacca cccccgaagc gcccgatgtg
     6721 caaaattacc tggcccaaat ggtggaggcg ggtatcacgc acgtcgtctt ggaggccacc
     6781 tcgcatgggc tggcccaggg gcgcgtgact gcctgcgatt tcgacctggg cgtggttacc
     6841 aatatcaccc acgagcacct ggattatcac ggcacgtacg aagcgtaccg ggaggcgaaa
     6901 gggcgcctga ttgcatccct ggcggatacc cccagcaaag cccaggggaa ttaccgcctg
     6961 gcagtgctca accgggatga ttcttctcat gattacctgg cgcagcttgt ccagcaacgc
     7021 atatcctacg gtatgcaccc gcaggctgat ttgagggggg ttgaactcga ggagcgcgcc
     7081 ggtggattgc gcttcgtcgc aaagggcgcg gggatgcgcc tggcagtctc cagcccgctg
     7141 ttgggcgaat acaatgtctc caattgcctg gcggcgattg gtgctacggt gtctgggttg
     7201 ggattggatc cgcaggctgc ccaggcagca attgcgaatt ttaccggtat atccgggcgc
     7261 atggaacgca tcgacctggg acaggacttc ctggcgatcg tggattttgc ccatacgccc
     7321 aatgcgctga aggtagcctt atccgctgcc aggaaaatgg ttggcagaag ggggcgtgtg
     7381 atcgccgtgt tcggttccgc agggctgcgc gaccgcatga aacggcgcat gatggcggaa
     7441 atctcggttg agctggcaga tctaactatt ctgacggcgg aagacccgcg caccgaatca
     7501 ttggacaaaa ttctggcgga gatggcagcc ggcgcagcgg ggcggggcgg ggtggaaggg
     7561 catacatttt ggcgcgtgcc cgaccgggga gaggcgatcc gccatgcttt acgcatagcc
     7621 aaagcagggg atgtcgtaat cgccctgggt aaaggtcacg agcaatcgat gtgctttggc
     7681 gagacggagt acaagtggga cgaccgcatc gccatgagag ccgcactcag cgagctgctg
     7741 gcgatccgcg gacccagcat gccttacctg cccacccaga gataagcaaa tttgagtgga
     7801 aatttggctt gatgcacctg ttgtataggg caatcgcatt agggcgattg cccaccactg
     7861 aggagagaaa atggggtgtt ttggtggggg tatgcccctc agggcataag caattgccct
     7921 gcctgttgta agcttcggat agcatttctt agaggggggt taatgttgct gaaaaacgct
     7981 gacaattact tgataatttc gatgatattt ggtataaaaa tttagacaat tgttgtatat
     8041 ccgtattgtc ttattccccc aatctaaagg agcaaacagt gaaaagaagc aatccaaccc
     8101 gaaggaaaat c
//
LOCUS       DBNGSX010000139         9657 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_980863, whole genome shotgun sequence.
ACCESSION   DBNGSX010000139 DBNGSX010000000
VERSION     DBNGSX010000139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9657)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9657)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9657
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_980863"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="AC16CB_04585"
     CDS             <1..992
                     /locus_tag="AC16CB_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HIR3341779.1"
                     /translation="VGRIGLVDYDVVDFSNLQRQVIHGTSRIGTLKVESARDRMLDLN
                     PDIQVDIYNEPFTSENAMRIAADYDLIIDGTDNFPTRYLTNDVCVLLGKPNVYGSIFR
                     FDGQVSVFYAKEGPCYRCLFPEPPPPGLVPSCAEGGVLGVLPGTIGSLQATEALKLIL
                     GIGDTLIGKLMLYNALDMTFEYVKLRKNPNCKICGPNPEVTELIDYEAFCGVPGHDHE
                     EGSVGGGWDITATELSQRLQQGNHIHLLDVREPHELQISKLEGADLIPLGHLAARLSE
                     LDSAQEMVVFCKAGTRSARALELLVSAGFRKVKNLKGGINSWARDVDPSMPIY"
     gene            1091..1927
                     /locus_tag="AC16CB_04590"
     CDS             1091..1927
                     /locus_tag="AC16CB_04590"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HIR3341780.1"
                     /translation="MSTLLAILAHPDDESFGMGGTLALYARRGMAVHLVCATRGEAGD
                     VDERYLRGFDSKAERREAELRCAAEKLGLASVHFLDYRDSGMPGMPDNQHPQALIAAP
                     QADVVAKVVHLIRELRPQVVLTFDPIGGYKHPDHITIHNATVQAFELAGQDRYVDGLP
                     AHQPDKLYFHVIPKGYLRFAVRVMPLLGKDPRRLGRNGDINLVSLVEDGDFPIHAVID
                     YRAVAELKDDASLCHASQLDGASLRRGPMRWAQVLFGQKDHFMQAYPLPENGRRKHDL
                     FV"
     gene            2310..2825
                     /locus_tag="AC16CB_04595"
     CDS             2310..2825
                     /locus_tag="AC16CB_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341781.1"
                     /translation="MLNKAYLFYILVIALVTAGCQPGESGDFAIYLLAQDVASVELAK
                     VDLDQLALEEGPIISAEDIVTYDQTTHEIELTQAAYARIQQIFPLPVKVDGIPFVVCV
                     GEQPIYAGAFWTPLSSLSFDGVVIMQPFDTDGTTIRIVLGYPGPDAFMGRDPRADERV
                     MKALQAAGKLK"
     gene            complement(2982..3284)
                     /locus_tag="AC16CB_04600"
     CDS             complement(2982..3284)
                     /locus_tag="AC16CB_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3341782.1"
                     /translation="MNEQLRDLVDIDRTIHEPARLAILAILWAAESVDFLYLLNTIEL
                     SRGNLSSHLSKLEAAGYILIQKTFEGKIPRTVCRLTDTGKAAFEGYKEQMRAFLAG"
     gene            complement(3281..3898)
                     /locus_tag="AC16CB_04605"
     CDS             complement(3281..3898)
                     /locus_tag="AC16CB_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341783.1"
                     /translation="MSKQINDFIRQTYQYFYIDGLPELASGSMYLVTGILLAVMTRVE
                     SGTLAAGLVGLALIPIILGGIYLQNRLLRHLKERITYLRSGYVSYRRERKDSRRWIVI
                     VVALLLPFVLTLLPEQWQDLPLIIGGILGSVHIYMGYRLSIKRFYIEGAFAVFLGIVA
                     AIFLNNEVLGAAIIFLGAGIVLMAAGGLTLRHYLQTHPLPDEVTG"
     gene            4282..5253
                     /locus_tag="AC16CB_04610"
     CDS             4282..5253
                     /locus_tag="AC16CB_04610"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3341784.1"
                     /translation="MNQIFRKLQVSEHFELYQIAEGVYAAIGLIGGAAYSNAGIIDLG
                     DRALIFDTFETPTAADDLRAAAEQLVGNQFSYIIISHAHTDHWMGNQSFPESVAIITT
                     QVIRDEMYEYGAEMRELQQNPSEFEEEIREYEERLQAETDKGQRASLQATISRYRYSL
                     EMLPDLKLRFPNQTFDGKLVFHGSQRVVELVASGKGHTASDCFLVLPAEKVAFIGDLG
                     FFQCQPYMVYCDPDAWVAQLEAMEAWDIETFVPGHGPVGSKVDLALQKEYIRTLNQLV
                     ARVIGEGGTVEDALRQPLPAPFDAWLPAGRARFEANVRSSYKRLSGG"
     gene            complement(5279..5869)
                     /locus_tag="AC16CB_04615"
     CDS             complement(5279..5869)
                     /locus_tag="AC16CB_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR3341785.1"
                     /translation="MKSWWGILWPISLGLMLGLLASGLILLVSSQPRGEAVRLSPPPT
                     APPLVVHVSGAVSQPGVFNLMQGSRVQDAIQAAGGITPDADAQALNLAAPLEDGVLVY
                     VPLKSQEGPATMSPGISAPSPAGNPGPTAGGLININTASQSELESLPGIGPVLAQRII
                     EYRQSHGAFSSTEAIIDVSGIGPGIFEKIRDLITVN"
     gene            complement(5929..6915)
                     /locus_tag="AC16CB_04620"
     CDS             complement(5929..6915)
                     /locus_tag="AC16CB_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012784.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIR3341786.1"
                     /translation="MVNKQPYKFTNELSEGPPPLDESWWDAVMAEEGAHKPTSPRSGS
                     TRPTTNDKASAAHTAEHPTPVDWDMAIELYESDKIVTLQVSGYNRGGLLVNGDGLQGF
                     VPVSHLVELPAEKSAAEQELALSEYVGRDLELKIIECDPSRGRVVYSERAALAEPGSR
                     NTLLHQLQSGQCVQGTVTNITDFGVFVDLGGVEGLVHVSEISWGRVRHPADVVRYGEP
                     LQVQVINVDPERSRVALSIKRLNQNPWETAETRYFPGQVTEAVITSLVPFGAFARLEE
                     GLDGLIHISEINSGNPPANLNNLLQEGQRVTVRVLHVDSANQRLGLSMVVQE"
     gene            complement(6915..8876)
                     /locus_tag="AC16CB_04625"
     CDS             complement(6915..8876)
                     /locus_tag="AC16CB_04625"
                     /inference="COORDINATES: protein motif:HMM:NF028237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HIR3341787.1"
                     /translation="MVDLFVSTLVTLNQLLTAGVAITAFSLLLYALTFNLRDRVARSF
                     AWILTCVVIVFASDAISSTTNSPADLVFWLKLQWVGIVFLPATYLHFSDGLLATTGRP
                     SRGRRRMVVRLSYGLSLAFLATLPFNLLVGSLVQGEQPAPHLDRTWLTWVFSIYFGIA
                     IAFAMINFARAYRRTLASTSRRRMGYLLVGALAPALGSFPYLLFGSGLASRHTLLFWI
                     IVTLSNLAISALLVLMAYAVAFFGVNWPDRVVKRRLFKWLMRGPVTASTVLAATTLAR
                     RAGESFGQSYTTAVPIIMVALILLLEYLITLAAPVWERWLFHGGDRENIEMLQNLEER
                     LLTLGDLRQFLESVLAAVCDRLQASNAFVAALGQNGLEMVVTIGSNHILKQEDLSANL
                     LEQVNQNGLHTNLFSWGGYWLVPLYAHQDDQDELLGLMGTKQQPGYEIDEEQQEALSI
                     LANRAALALEDRRAQEQLFTSVEALTPAVELIQNLRAATRYGGGGALGELLTQPELSK
                     EKGAFSSWVKDALTHYWGGPKLSQSPLIKLRVVQQALEEHEGNPTNALRAILRQAIDR
                     VRPEGDRRFTAEWILYNILELKFMEGRKVREVALRLAMSEADLYRKQRVAIEEVAKAI
                     IDMETIAVDEDNVAMGVATTEANHYTRWA"
     gene            complement(8919..9458)
                     /locus_tag="AC16CB_04630"
     CDS             complement(8919..9458)
                     /locus_tag="AC16CB_04630"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3341788.1"
                     /translation="MPEIQIRPAIASDIPAIMAIDHNYSSDYVWQMEIQSEEGQVGVS
                     FRQIRLPRSVRVEYPRPISQLEHDWTQRPGLLVASLNEETVGWIGLRLDIAPVTAWAT
                     DLGVLRRLRRHGIGSALVLAAQEWGETHDCRRLVLEMQPKNYPAIQMAHKLGLDFCGY
                     NDRYYANHDIGLFFAKSLR"
     gene            complement(9473..>9657)
                     /locus_tag="AC16CB_04635"
     CDS             complement(9473..>9657)
                     /locus_tag="AC16CB_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341789.1"
                     /translation="WLTILAGNEVKVDDTLGAWNTSLLTPGDHELSLVVTDNQGNSLS
                     PCIIGVRVINPVAPQP"
BASE COUNT         2144 a         2618 c         2612 g         2283 t
ORIGIN      
        1 gcgtggggcg tatcggtctg gtggattatg acgtggtgga tttctccaac ctgcaacgcc
       61 aggtcatcca cggcacctct cgcattggca cactaaaagt cgaatcagcc cgcgatcgca
      121 tgctggacct caatcccgat atccaggtag acatctataa tgagccgttc acttcggaaa
      181 atgccatgcg catcgctgct gattacgatc taatcatcga tggcacagat aatttcccca
      241 cccgctacct gaccaatgac gtgtgcgtgc tgttgggcaa acccaatgtg tatggctcca
      301 tcttccgctt cgacggccag gtgagtgttt tttacgccaa agagggccct tgttaccgct
      361 gtctcttccc tgaacccccg cctccgggat tggttccttc ctgcgctgag ggtggcgtgc
      421 tgggtgtgct cccaggcacc atcggatcgc tgcaggctac agaggcgctc aagctcatct
      481 tgggtattgg agacacgctc atcggcaagt tgatgttata caatgccctg gatatgacct
      541 ttgagtatgt caagctgcgc aagaatccca attgcaagat ctgtggaccc aatccggaag
      601 tcacggagct gattgattac gaagccttct gcggcgtgcc cgggcatgac catgaagagg
      661 gttcggtggg cgggggctgg gacatcacgg ccaccgagct gtcccagcgc ctgcagcagg
      721 gcaaccacat ccatttgctg gatgtgcgcg agccgcacga gctgcagatc tcgaaactgg
      781 aaggcgccga cctcatccca ctgggacatc tggcggcgcg cctgtccgag ctggacagcg
      841 cccaggagat ggtcgttttc tgcaaagccg gcacccgttc agctcgtgcc ctggagttac
      901 tggtcagcgc tggtttccgc aaggtgaaaa acctgaaggg tgggattaac tcgtgggcgc
      961 gcgacgtcga tccaagcatg ccgatctatt agtgattttg gattaaagat tggtgattga
     1021 agatatattc gctcattaat caacaatcgg caatctgcaa tcgacaatct tgaaatttgc
     1081 atcaatgaac atgagcactc ttcttgctat tcttgcccac cctgacgacg agtcgtttgg
     1141 gatgggtggc acattggccc tttatgcccg gcggggtatg gcagtgcacc tggtttgtgc
     1201 cacgcgtggt gaagctggtg atgtggatga acgctatctt cgggggtttg actccaaagc
     1261 cgagcgccgc gaagccgagc tgcgctgcgc tgccgagaag ctggggctag cgagcgtgca
     1321 tttcctggat taccgcgatt cgggcatgcc cggcatgcct gataaccagc accctcaggc
     1381 cctcatcgct gccccgcaag cagacgtggt ggcgaaagtg gtacatttaa tccgtgagct
     1441 acgccctcag gttgtcttaa cctttgatcc tattggcggc tacaagcacc cggatcatat
     1501 cactatccac aatgctaccg tgcaagcctt tgagcttgcc gggcaagacc gctatgtgga
     1561 tgggctgcct gcccaccagc cagacaaact ctattttcat gtcatcccca aaggctacct
     1621 gcgctttgcc gtgcgtgtga tgcccttgct gggcaaagac ccgcgccgtc tcgggcgtaa
     1681 tggcgatatt aacctggttt ccctggtgga ggatggggat tttcccatcc atgctgtgat
     1741 tgactatcgc gccgtggctg agttaaagga tgatgcctca ttgtgccatg ccagccagct
     1801 cgacggcgcc tcgctgcgcc gcgggccgat gcgctgggcg caggtgctct ttgggcagaa
     1861 agatcacttc atgcaggctt atccgctgcc cgagaacggt cgccgtaagc acgacctatt
     1921 tgtgtgaaat gatgagacga ccttcgattt ccgatttctt gaccaatttg gaccaaccca
     1981 tgccctttcc agagaagatg gcaaagctta ctcgcaacct ctggcgcagg gtgttgttgc
     2041 gccagaactg ttgtgggcat gatggtgagc caggctgttg aaaagcagat ccctcatccc
     2101 gtgaggatga acaccaccat caccaacact agccatttaa ccgctttgcc cagctggtgg
     2161 gtccgcttgg atcggtgaca ttaatcccct ataagacaag catcctcttc tcctcacccc
     2221 ctggggtgca ggggaagcgc cacggtgaag tgcagtgcta ccacagcggg ggtaagatca
     2281 ttgtaaaatg tggtcctcag gaggcattcg tgttgaataa ggcatatctc ttttatatcc
     2341 tcgtcatcgc tttggtaacc gctgggtgcc agccaggaga aagcggcgat ttcgccatct
     2401 atttgcttgc ccaggatgtg gcctctgtgg agcttgccaa ggttgatctt gatcaattag
     2461 cccttgaaga ggggccaatt atctcggcgg aggacattgt cacctacgat caaaccaccc
     2521 acgaaataga gctcactcag gccgcctatg ctcgcattca gcagatattc cctttgccgg
     2581 ttaaggtgga cggcattccc tttgtggttt gtgtggggga gcaacctatc tatgccggtg
     2641 cattctggac gccgctatcg tccctcagtt ttgacggtgt tgtcatcatg caaccctttg
     2701 acactgacgg gaccacgatt cgaatcgtat tgggttatcc cgggccggat gcatttatgg
     2761 gaagagaccc gcgcgccgat gagcgggtga tgaaagcctt gcaggcagca ggtaagttaa
     2821 agtagaacgg ggctgttgtg tgaggccctt ttctattcca gctcgttcct ggatctcgga
     2881 atggggggca ctgggatcac cgtaatctcc ccaactgcgg tttgacgcat cgcctcgaca
     2941 tcccacgtct ctagctgcca gagcgatgca caagttgatc tttagcccgc caggaaggcg
     3001 cgcatctgct ctttatagcc ttcgaaagcc gctttgcctg tatccgtcaa gcgacagact
     3061 gtccggggaa ttttcccttc aaaggttttt tggatcagga tatatccagc tgcttctagc
     3121 ttgctgagat gggaagacag gtttccccgg ctgagctcga tggtgttcaa caggtataag
     3181 aaatccacgc tctccgcagc ccataatatc gccaggatag ccagccgggc tggctcgtgt
     3241 atggtgcggt cgatatcgac cagatcgcgc agctgctcgt tcaccctgtc acctcgtcag
     3301 gcaaaggatg agtttgtaga tagtgtcgca gcgtcagccc gccggctgcc atcagcacga
     3361 tgccagcacc aagaaagatg atcgctgcac caagcacttc attgttcaga aatatggctg
     3421 cgacaattcc caaaaaaacg gcaaacgcac cttctatgta gaagcgcttg atgctaagcc
     3481 ggtatcccat ataaatatgc accgagccca ggattcctcc aatgatcaga ggaaggtctt
     3541 gccattgctc aggcagcaag gtcagaacaa acggaagcag caaagcgaca acgatgacga
     3601 tccaacgcct gctatctttg cgctcgcgtc ggtaggagac atagccagat cgcagataag
     3661 taattcgctc cttgaggtgt cgtagaagac ggttttgcag gtaaatcccc cccaggatga
     3721 tggggataag tgccagccca accagacctg cggcgagcgt tccagactcg acgcgggtca
     3781 tgactgccag taagatacca gtaaccaggt acatgctgcc gctggctagt tctggcagcc
     3841 catcgatgta aaaatactgg taggtctggc ggataaaatc gttgatttgc ttgctcatga
     3901 ttgactccgg ttatataaca cgaactagtt tgtattgcaa acttatttgt attattttag
     3961 cagatggttg tagaattgtc aagggctgtt tcttactcta tctggctgtt ttcacaggaa
     4021 agctgtgttt ctctatctgt gtcagccgaa gtgcttgccg aatctctgtt tgtgcaattc
     4081 ccttgctttc ttcccgcatt caatggtata cagatagagc gctttacctg gtaaagtatt
     4141 tgtgtcgatt tggagttcgt tcccgtcgca caatgggttt atctcatcca gtattggtct
     4201 gcatcaccca ttctgagcgg catcctgtat gaggttttta gacgatccaa atttctctgt
     4261 ggttcgagag gtgagttaaa tatgaaccag atttttcgta aactccaggt ttctgaacat
     4321 tttgagctct atcaaatagc cgagggggtc tacgccgcga tcggccttat tggtggtgcg
     4381 gcatacagca atgctggcat catcgatctg ggcgatcgcg cattgatctt tgacaccttc
     4441 gagacgccca ctgcagcaga tgatttgcga gctgccgctg agcaattggt cgggaatcag
     4501 ttcagctaca tcattatcag tcacgcccac acggatcatt ggatgggcaa ccaatctttc
     4561 cctgagtcgg ttgccatcat caccacccag gtgatccgcg acgagatgta cgaatatggc
     4621 gctgaaatga gagagcttca gcaaaatcca tcagagtttg aagaggagat tcgggagtat
     4681 gaagaacgcc tccaggcgga gacagacaag ggccagaggg catctctcca agctacgatt
     4741 tcacgctatc gctattcact tgagatgttg cctgacctca aacttcgatt tccaaaccaa
     4801 acctttgacg ggaagctggt atttcatggc tcgcagcggg tagtggaatt agtcgcctca
     4861 ggcaagggcc acactgccag cgattgcttt ctcgtattgc cagccgagaa ggttgccttt
     4921 ataggggatt tgggattctt tcaatgccag ccctacatgg tttactgcga tcccgatgcc
     4981 tgggtggccc agctagaagc gatggaagct tgggacattg aaaccttcgt gcccgggcat
     5041 ggtccggtgg ggagcaaagt ggacctggca ttgcagaaag aatacatccg cacgctgaat
     5101 cagctggttg cccgtgtgat tggagaaggt ggaacggtgg aagatgcgct gcgccagccg
     5161 cttcccgcgc cgtttgatgc gtggttgcca gccggaaggg cgcgctttga agctaatgtg
     5221 cgctcatcct acaaacgact ttccggcgga tgagcaacaa tccagccagc gcaaccgttt
     5281 agttcaccgt gatgaggtct ctgatcttct caaatatccc tggtccaatg ccagagacat
     5341 ctataatcgc ctcggtgctt gaaaaagctc cgtgactctg ccggtattcg atgatgcgct
     5401 gtgccagcac tggaccgatg cccggcaggc tctccagctc gctttgagag gccgtgttga
     5461 tattgatcag gcctcccgct gtcgggccgg ggtttcccgc gggggaggga gcggatatgc
     5521 ccggtgacat tgtggcaggc ccctcttggc ttttcagggg gacatagacc agcaccccat
     5581 cctccagggg agcggctagg ttgagggctt gcgcatctgc atctggggtg atgccccccg
     5641 cagcctgtat cgcatcctgg acgcggctgc cttgcattag attgaagacg cccggttgag
     5701 aaacagcgcc gctcacgtgg acgacgagag gtggcgctgt gggaggtgga gagaggcgga
     5761 ccgcctcgcc gcgtggctgg ctgctgacca gcaagattaa acccgaagca agcaagccaa
     5821 gcattagccc caggctgata ggccacaaaa taccccacca ggacttcatg gcactttttt
     5881 accttcccct acccaagtcg ctcccccgtg attgggggat gagagatatt actcttggac
     5941 gaccatactc agccccaggc gctggttggc ggagtccaca tgcagcacgc gaaccgtaac
     6001 gcgctgtccc tcctgcagta agttattcag gtttgccgga gggttgcctg agttaatttc
     6061 ggagatgtga atcagcccgt ccagaccttc ttcaaggcgg gcaaaggcgc caaagggtac
     6121 caggctggtg ataaccgcct cggtaacctg cccggggaaa tagcgcgttt cggctgtttc
     6181 ccacgggttc tggtttaacc tcttgatact gagagccact cgagagcgct ctgggtctac
     6241 attgatcacc tgaacctgca gtggctcccc atatcggaca acatccgctg ggtgccgcac
     6301 gcgcccccaa gaaatctcgg atacgtgaac cagcccctcc acgcccccca gatctacaaa
     6361 gactccaaaa tcagtgatat tggtgactgt gccctgcacg cattggccgg attgcaattg
     6421 gtgtagcagc gtgttgcggc tgcctggctc agccagtgcg gcgcgttcag aatagaccac
     6481 cctccccctg gaagggtcac actcgataat cttaagttct aaatccctgc cgacatactc
     6541 gcttagggcc agttcctgct ctgcggcgct tttttctgca ggcagctcga ccaggtgcga
     6601 aacgggcaca aagccttgca atccatctcc attgaccagc aatccacccc ggttatatcc
     6661 gcttacttgc aaggtgacga tcttatctga ttcatacagc tctatagcca tgtcccagtc
     6721 gactggagtt ggatgttcag ccgtgtgcgc agcacttgct ttgtcatttg tcgtgggccg
     6781 ggttgacccg gacctgggtg atgttggttt atgagcaccc tcttccgcca tgactgcatc
     6841 ccaccaagat tcatctagcg gaggtggacc ctcgctcagt tcgttagtaa atttgtaagg
     6901 ttgtttgtta accattaagc ccaccttgtg taatgattag cttctgtcgt agcgaccccc
     6961 atcgccacat tatcttcatc gacggcgatt gtttccatat ctataatagc tttggcgacc
     7021 tcctcgattg ctacccgttg cttacgatac aggtcagctt cagacatggc gaggcgtaaa
     7081 gctacctcgc gcactttgcg accttccatg aatttcaatt ctaatatatt atacaaaatc
     7141 cattctgctg taaagcgtcg atctccctcc ggtctgactc ggtcaatagc ttggcgcagg
     7201 atagctcgca aagcgttggt ggggttcccc tcgtgttcct ccagcgcctg ttgcaccacg
     7261 cgcagtttga tcaatggact ctgagatagc tttggacctc cccaatagtg agtaagtgca
     7321 tctttaaccc aactagagaa ggcgcccttc tctttggaga gctcgggctg agtgagtagc
     7381 tctccgagtg cccccccacc gccgtagcgc gtggctgcgc gtagattttg gatcaattct
     7441 accgcaggtg tcaatgcttc gacagaggta aataattgct cctgcgcccg ccggtcttct
     7501 aatgccagtg cagcccggtt tgccagtatc gagagcgctt cctgctgctc ttcatcaatt
     7561 tcatatccag gctgctgttt ggtccccatc aagcccaata gttcatcctg atcgtcttga
     7621 tgggcataca ggggtaccag ccagtatccc ccccatgaga acagatttgt gtgcagaccg
     7681 ttttgattga cttgttccag taaatttgcc gatagatcct cctgtttaag aatgtgattg
     7741 ctgccgatgg taactaccat ctctaacccg ttctgtccaa gtgccgccac gaaagcattg
     7801 gaggcctgca agcgatcgca caccgcagcc agcaccgatt ccaggaactg cctcagatcc
     7861 cctaaagtca gcagccgttc ttctaggttt tgcagcatct cgatgttttc gcgatcgcct
     7921 ccatgaaata gccagcgctc ccatacaggt gctgcgagag tgatcaggta ctccaggagg
     7981 agaatcaagg ctaccataat gatgggcacc gcggttgtgt agctttgccc aaaggactcc
     8041 ccagcccgcc gggcgagcgt ggtggcagcc agcacggtgg aggcggttac cggaccccgc
     8101 attaaccact tgaaaaggcg tcgcttgacc acccggtcgg gccagtttac cccaaagaat
     8161 gccaccgcgt atgccattaa gaccagcaac gcagagatag ccaggttgct taatgtgaca
     8221 attatccaga aaagcagtgt atgtctgcta gccaggccag aaccgaataa caggtacggg
     8281 aaagagccca aagcgggcgc aagcgcccct accagcaggt agcccatacg ccttcggctg
     8341 gtgctcgcca gggttcgtcg gtaggcgcgc gcaaagttga tcatcgcaaa ggcgatcgcg
     8401 atgccgaagt agatgctgaa gacccaggtg agccaggtgc ggtccaggtg aggcgcaggt
     8461 tgctcgccct gtaccagcga accgacaagc aggttgaagg gcaatgtggc taagaaagcc
     8521 agtgaaaggc cataggacag gcgcacaacc atgcggcgtc ttccacgcga tggacgtccg
     8581 gtggtggcta agagtccatc ggaaaaatgc aggtaagtgg ctggcaggaa aacgatacct
     8641 acccattgga gcttgagcca aaataccagg tctgcaggcg aattggtggt ggatgatatg
     8701 gcatcgctgg cgaagacgat gactacacat gtcaggatcc aggcgaatga gcgcgccact
     8761 cgatcgcgca aattaaaagt cagcgcgtat agcagtagcg agaatgccgt gatggcaact
     8821 cccgcggtga gcagttggtt aagagttacc agggtagaga caaacagatc gaccatacct
     8881 tgttaatagt ttactacggt tcaagatggc atagcatact atcttaaaga ctttgcaaaa
     8941 aataaaccaa tatcatgatt cgcgtagtag cgatcgttgt agccacaaaa atctagacct
     9001 aatttatgcg ccatctggat agccgggtag tttttgggct gcatctccaa aacaagccgc
     9061 cggcagtcat gcgtctcgcc ccattcttga gctgccagca ccagagcgct gccgatccca
     9121 tggcggcgca gccggcgcaa cactcccagg tcggtagccc aagcggtgac cggggcgatg
     9181 tccagcctca aaccgatcca gcctaccgtc tcttcattta gcgaggccac caataatcca
     9241 ggtcgttgtg tccaatcgtg ttccaactgg ctaatgggtc tgggatattc tactcgcaca
     9301 gagcgtggca ggcgaatttg gcggaagctg acacccacct gtccttcttc agactggatt
     9361 tccatctgcc atacataatc gctggagtag ttgtgatcaa ttgccatgat cgcggggata
     9421 tcggaagcta ttgctgggcg aatttggatc tcaggcatca ataccacctt tattagggtt
     9481 ggggtgccac ggggttgatc acccgcaccc cgattataca gggagagagg gagttccctt
     9541 ggttgtcagt gaccaccagg ctgagttcgt gatcacccgg ggtgagcaag ctggtgttcc
     9601 aagctccgag cgtatcatct actttgacct cattcccggc caggatggtc aaccagt
//
LOCUS       DBNGSX010000140        15238 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_149580, whole genome shotgun sequence.
ACCESSION   DBNGSX010000140 DBNGSX010000000
VERSION     DBNGSX010000140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 15238)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15238)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15238
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_149580"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="AC16CB_04640"
     CDS             complement(<1..592)
                     /locus_tag="AC16CB_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341790.1"
                     /translation="MAFIIPARAQDEVELTLNLTRDFGYSSGTGRIQGRFTMKVNGPA
                     NLERVVFLIDDLMVGEDTQAPFLLQFSTGDYALGVHTMSAVGYTSDGRELRSNAQRRE
                     FVSAEEGWQSAMKIAIPIIGLAFAAMLFSFILPSLLGKRVSMATPLGAPRSYGLLGGT
                     VCPKCSRPFGMHVWGMNLVIGKLDRCPYCGRWSLVRR"
     gene            complement(651..1439)
                     /locus_tag="AC16CB_04645"
     CDS             complement(651..1439)
                     /locus_tag="AC16CB_04645"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR3341791.1"
                     /translation="MNKIKTIILKEWAQVFKNRLVLSTLVFMPLLFTAIPLMMLYATR
                     GTGAQELSSDMPPQFTSMCSQGLTAGECFQVYIVNQFMMLFMMIPLIIPVNIAAYSIV
                     GEKTTRSLEPLLATPITTAELLLGKNLAAAIPAVVATWMGFLLYVLGAWIIVQSSRVV
                     AALLDPMWWLAVFVAGPLLAVLSVNFALMVSSRVNEPRVAEQLSAIVILPLLALFIGQ
                     IAGLFLINRQLVLLFTIILALIDALMIYVAIRLFQREVILTRWK"
     gene            complement(1436..2338)
                     /locus_tag="AC16CB_04650"
     CDS             complement(1436..2338)
                     /locus_tag="AC16CB_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3341792.1"
                     /translation="MIQTHNLTKEFNGTLAVERLNLEVGEGEVFGFLGPNGAGKTTTI
                     RMLTSLIAPTSGGAIVNGYTIGEDDRKIRQTVGILTESPGLYDSLSAEYNLQIYANLY
                     EVKDVRGQVEKYLRMLGLWDRRQDEVGTFSKGMKQKLAIARAILHEPRILFLDEPTAG
                     LDPEASHLVREFIEELRKQGRTIFMCTHNLDEADRLCDKIGVFKTRLLTLDTPTNLRT
                     QLFGRKVVFHLSHVEETYLALLRSMPFVKEARAVDNKLVATLDDPENQNPEIVRNLVE
                     AGAEIRFVGELRHSLEDVYLQLVK"
     gene            complement(2335..3210)
                     /locus_tag="AC16CB_04655"
     CDS             complement(2335..3210)
                     /locus_tag="AC16CB_04655"
                     /inference="COORDINATES: protein motif:HMM:NF021592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HIR3341793.1"
                     /translation="MPPTLAYPLSAFLMIALPLGLGIALERKFRLGWRLWWIGGAGFV
                     LSQVGHIPFNIGLTVLFQRGILPAPPDSAKLVFNSTILGLSAGLWEELTRYAVFRWWV
                     KDARSWRKGVWLGAGHGGIEAVLLGIIVFANFFYQMAIRNVDLSTMVPQNQLAIAKDA
                     VAAYWSAPWYLILLGALERAMTLPIQICFSVLILQSFIRGQIGWLFLAVGWHAIIDSV
                     VVFSASTRGVYIAEFLVAVACLISLTIIFVLRKPEPQPMEDSTVDAFQPEPPQVIDHN
                     IIETPEKIDRTRFIE"
     gene            3547..4506
                     /gene="pdhA"
                     /locus_tag="AC16CB_04660"
     CDS             3547..4506
                     /gene="pdhA"
                     /locus_tag="AC16CB_04660"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HIR3341794.1"
                     /translation="MEKNEYLDLYYQMVLIRIVEEQAAQLYQQGKIGGFLHLYIGQEA
                     VSTGLISVRQPQDRVITAYRDHGVAINCGISAKEVMAELLGKATGISKGKGGSMHMAD
                     VKKNFWGGHAIVGAHLPLAAGLALGDQYQGNDGVTICMFGDGATNIGYFHEALNLSKV
                     WNLPVLWVCENNQYGMGTAVERASAVSEIRQKAEGYAMANGRVDGMDIMAVREASRQA
                     LEQVRSGQGPYFLEVITYRYRGHSMGDPERYRQSDEIRKWQENDPIGIYRGYLTGQKI
                     ASEEELDQIDSQVGDEVQQAVQFAEASPEPAPEALFEHVYVEG"
     gene            4519..5490
                     /locus_tag="AC16CB_04665"
     CDS             4519..5490
                     /locus_tag="AC16CB_04665"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR3341795.1"
                     /translation="MSKITMREAISKALWEEMERDSKVFILGEEVGVWGGTYAVTKGF
                     VDHFGPDRVRDTPIAEAAIVGAAIGAALTGLRPVAELMTINFAFSAMDHIVNEAAKLH
                     YMFGGQLVLPVVIRTVSGGGRQLGATHSQTPDAIFAHFPGLKVVAPGTPEDAKGLLKS
                     AIQSDDPVLFIEHATLYQVRGEVPDGDYTIPIGKSKIQRPGRDVTIVTYSKMLEISTK
                     AAEQLSQEGIEVEIVDLRSLRPLDMEPVLESFKKTNRAVIVEEGWRSYGVGSEVAARI
                     YEQAFDYVDAPIQRVAQEEVPLPYNRNLEQLALPQVEDVVRAVKEVL"
     gene            5490..6737
                     /locus_tag="AC16CB_04670"
     CDS             5490..6737
                     /locus_tag="AC16CB_04670"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010507946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIR3341796.1"
                     /translation="MAETVTMPKLGFDMAEGTLIRWVIAEGEAVNKGAILAEIETDKA
                     TVEVESVYDGILARHLVAEGDIVPVNTPIAIVAAPGEKVEKQPQLVTDQAAPTPKSAA
                     EAEPISAPSQAPSEPTVVEGQLPEGVKASPLARRMAEERGIPLRSISGSGPGGRITKK
                     DVEAYKAVQAAPTQPQVAFQAGLPSIPSTTIPLKRLRAIIGRRMTEAKQSVPHFYVTH
                     EYDMAALLALRQQVNLTLPDEEKTSVNDFILKASALTLREFPNLNASLDDNNNQIIQH
                     GAVNIGVAVAVEGGLLTVVCRDADRKPIRQISAEVRQMVQRAREGKVRPDDIEGSTFS
                     VSNMGMYDVEHFIAIINPPEAAILAVGSAREVPVVKDGEVVPGLRMKATLSVDHRVSD
                     GAEGARFMQTLARYIEEPIRLLL"
     gene            complement(6805..8418)
                     /locus_tag="AC16CB_04675"
     CDS             complement(6805..8418)
                     /locus_tag="AC16CB_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341797.1"
                     /translation="MTGDLSEKNTSKFEMREQIKPRVRIVRFTLDHPEVRTFLSIIGM
                     TLIFIALLSIVQFSTDNLAGVDGYFHIRFASIMRTEGLKPGFPWLPLTILNPREYSDH
                     HLLFHIALIPFTLGDLREGAKLAAVLFPAIAFLCIWWLLRVQRVPYSALWALGLLAIS
                     EAFIFRMSLVRRQSLTLAVMAVALHWMLTKKYVRLIPLGFVYVWLYDAFPLLLTLTTI
                     YVASILLIERRLELRPLIYCGVGVTLGLVLNPYFPNDIIFAIRHLAPKITETTALDVG
                     NEWYPYQTSQLLENSPLTLVAFFSGVLALGLRERRMETRTATSLFIALLFGLLLLQSR
                     RFIEYFPPFALIFAAFAWSPLLQLQDTNQDDINVSDPSLRFLKYSILGLRWRQVTAVV
                     VLVALLIPGIWLTVRDAQKSVKSSKPYTLFSGASEWLKANTPSGSRVFQTDWDDFPRL
                     FYHNTHNTYLIGLDPTYMQLYNADLYDLWEKITEGDVERPSQFIYDRFGANFVVSDLN
                     HKDFIRRAEEDPGLEEVYRDEESVVYAFK"
     gene            complement(8507..9790)
                     /locus_tag="AC16CB_04680"
     CDS             complement(8507..9790)
                     /locus_tag="AC16CB_04680"
                     /inference="COORDINATES: protein motif:HMM:NF039823.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HIR3341798.1"
                     /translation="MTTLNDHEQPIDPRLKELLDSLRETPARNPEAEARGRAKYLSQL
                     DALFDEDTRPVIHQAGISAPKESRSKWGFSLASLQQRLAFTTFMAVLAIILLLFGGAG
                     ATAYASQGALPGDALYPVKTSLELTQVRLARDAARQAELHLSFAERRLVEIASLIAEG
                     DFDHIETATREFETHVQEAIRALETVSAGDPQRAGELAIQITSALTRYAEILKGMLIS
                     VPDTIKPSVEKAILISEQEEHEDEIEFTGLIESISDEGYRIAGQLVVITPETEIKGSL
                     DIGAMVKVHATQAADGTLTAREIEVTLASDENLNGNENTNESDDITNANDNLGTANLN
                     VNEIENENETMNASNENEGLQHGIGNQNMNDNTGSGTSGSGSQTNTNNNDNSGGSGGN
                     SNDNSGSGGNSNDNSGSSGGSNDNSGSGGNSNDDD"
     gene            complement(9798..10337)
                     /locus_tag="AC16CB_04685"
     CDS             complement(9798..10337)
                     /locus_tag="AC16CB_04685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3341799.1"
                     /translation="MQIDTTQRPQAKILTKQDMINSYEQYSPGIYRYAYRLLGDQDLA
                     EDCVSETFSRFIQAIRNGGGPSENVQAYLYRVAHNYITDHYRRQPPPPLSLEEDLLAD
                     NDTNPMQLMTKNIEQERVRRAMLQLPPEQRRVIMLRVFEDWSHEEVSAVLGKTVEATR
                     ALQYRALAALRRLLIENEA"
     gene            10687..11262
                     /locus_tag="AC16CB_04690"
     CDS             10687..11262
                     /locus_tag="AC16CB_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341800.1"
                     /translation="MPGPAYEISYLQNTLELLEKYVLSADIYWPVRSAPEAGELPFPS
                     LTLGGVLLSLARLNSTTISDQHQAMREQLRGDIESIAIRWRVAWESKAVREFHARLIL
                     WRDYLDEYRSNPENNTDRYSYEVNRRVMLHLLEPYVEHAPEAEKEMLRGLDQLLKATF
                     QAGEFIWDAWLESAFPPQPYWYLFGKLRGSK"
     gene            11259..12491
                     /locus_tag="AC16CB_04695"
     CDS             11259..12491
                     /locus_tag="AC16CB_04695"
                     /inference="COORDINATES: protein motif:HMM:NF024241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3341801.1"
                     /translation="MTFIRWSPAPVGSTPVQRKNFINVQIDAIGIGLASALAPFLPVF
                     LTRLGASSVQVGLLTSMPAFTGLLLAIPLGRFLQTRRQIVPWFGAARLMVVSSYAMTG
                     LITFFVPEKYLVNSILLIWAAATLPQTIVAVCFSVVMNAVAGPQHRYELMSRRWSILG
                     VTTALTVALVGQMLNRIEFPLNYQFAFLTLSIGGLISFYYSSHIRLPDAVLEETARGI
                     SWRDRFRDYIRLITSEKAFVNFSLKRLFFMFGFTLAVPLYPLYYVREVHANDAWIGII
                     STVQSAVLLVGYFLWTRGSRLRGSRFVLLRTTFGLAVYPALVALTTRVELIALFAGLA
                     GIFQAGLDLVFFDELMKTVPPQYSATFVSLAQSFQHLAAMLGPMLGTLLADHFGLGIG
                     LFVSAILRLLGFILFSRR"
     gene            12628..13743
                     /locus_tag="AC16CB_04700"
     CDS             12628..13743
                     /locus_tag="AC16CB_04700"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR3341802.1"
                     /translation="MDASLRLGKIWEIPIGLHTSWFLVFGLLTWSLASGYFPEEYPNL
                     SSIVYIILALVTSLLFFGSVLAHELGHSFVAIRNRIPVKGITLFIFGGVAQISKEPGS
                     PGEEFRIAIAGPIVSLLLAGAFGVVWLFDQSIPILAAPSMYLLRINFMLAAFNMIPGF
                     PLDGGRVLRAIIWRINGNFQKATQTASYAGQLVAFGFIGWGVFTIFNGQFLNGLWLAF
                     IGWFLQNAAATAYAQTNMQSTLRSIHVSQVMERDCPRVPRLLTINQLVEDHILTGGQR
                     CFFVADDERLLGMLTLRDITQIPRPKWRFTTTEQAMIPLRNLVSVEPNTQLMSALQTM
                     DNAKINQLPVLIGEELVGMLSREQIVHYLRTRTELGV"
     gene            13750..14967
                     /locus_tag="AC16CB_04705"
     CDS             13750..14967
                     /locus_tag="AC16CB_04705"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR3341803.1"
                     /translation="MEATPFLQLIIALGVIIVAAKLGGYASYSLGQPSVLGELLVGII
                     IGPSLLNLLHLPYFSDEHLVETVAHLGEIGVLLLMFIAGLELHLDELAKTGKVAVFAG
                     ILGVVVPLVLGTGLGLLFEFDIAPAVLVGLVLSATSVSISAQTLMELKVLRSRVGIGM
                     MGSAVFDDILVILGLSLLFAVTHSGGNASIFGILWIALRMVLYLVGAWLVGAWLLPKL
                     GAWVEKLPISMGLISFAFVIMLVYAWAAETLGGMAAITGAFLAGLLLARTRLKERIDT
                     GIATISYAIFVPIFFVNVGLSANARLLTVDTAWFFILITILAIIGKVLGAGLGANLAG
                     FSRLEALQLGVGMMSRGEVGLIVATVGIAEGLLFPEAFASIVGMVIVTTLITPPLLRV
                     LFTRPLNKKPSEA"
     gene            14987..>15238
                     /locus_tag="AC16CB_04710"
     CDS             14987..>15238
                     /locus_tag="AC16CB_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341804.1"
                     /translation="MAYLIVLIIDDPDDCPAILEGWEAIGIGGITILESSGLGRFRRA
                     GMLDNLPLMPSMRDLFEKGETHHRTLLSVVEDQEKVDQMV"
BASE COUNT         3645 a         3541 c         4057 g         3995 t
ORIGIN      
        1 cgcggcgcac caggctccat ctaccgcagt aggggcagcg gtctaatttg ccgatcacca
       61 gattcatgcc ccatacatgc atcccaaatg ggcggctaca ctttgggcag accgtgccgc
      121 ccaacaaacc gtaagaacga ggcgcaccca agggtgttgc catgctgaca cgcttcccta
      181 agagactcgg caggataaaa gaaaacagca tggcggcaaa tgcaaggcca atgatgggaa
      241 tagcgatttt catggcagac tgccagcctt cttctgcgct gacgaattca cgccgttggg
      301 cattagatcg cagctcgcgc ccatcgctgg tgtatcccac tgcgctcatg gtgtgaacac
      361 ccagcgcata gtcgccagtg gaaaattgaa gcaagaatgg cgcttgggtg tcttcgccaa
      421 ccatcagatc atcgataagg aatactactc tctcaagatt tgcaggcccg tttaccttca
      481 tggtaaacct gccctggatg cgaccagtcc cagaagagta accgaagtcg cgggtcaagt
      541 tcagggtaag ctcaacttca tcctgggccc tggcagggat gataaaagcc atcccaaata
      601 ccatcaatat taaacataac ttggttagtt tcttcatcga ttatgaggtg tcatttccag
      661 cgggtcaata tcacctcgcg ctggaataat cgaatagcca catagatcat tagcgcatca
      721 ataagtgcca gaattattgt aaaaagtaaa acgagttggc ggttgattaa gaaaagcccg
      781 gcaatttgcc cgatgaaaag tgccaggagt ggcagaatca caatcgctga aagttgttct
      841 gccacgcgcg gctcgttaac cctagaagag accatcaggg cgaaattcac ggataagacg
      901 gctagcaatg gccctgccac gaatactgcc agccaccaca tcgggtccaa caatgcagcc
      961 acaacacggg atgactgcac gatgatccat gcgccgagca catagagcag aaagcccatc
     1021 catgtcgcta ccacagcagg gatagctgcg gcgaggtttt tacccaagag caactcagcg
     1081 gttgtgatgg gtgtagccaa gagtggctcc aggctgcgtg tggttttttc ccccacaata
     1141 gaataggccg cgatattgac cggaatgatc aaggggatca tcataaatag catcatgaac
     1201 tggttgacta tgtagacctg aaagcattca ccagcagtaa ggccttgtga gcacatggat
     1261 gtaaattggg gcggcatatc gctgcttagt tcctgcgcgc ccgttccgcg cgttgcgtaa
     1321 agcatcatca acggaatcgc tgtgaagagc agcggcataa ataccagggt tgagagcacc
     1381 aggcggtttt tgaacacctg ggcccattcc ttgaggatga tggtcttaat tttgttcatt
     1441 tgacaagttg taggtacaca tcttccaaag agtggcgcag ctcgccaaca aagcgaatct
     1501 ctgcgccagc ctctaccagg ttacgaacga tttccggatt ttggttctcg ggatcgtcca
     1561 aggttgccac cagtttattg tcgactgccc tggcttcttt gacgaagggc atcgatcgga
     1621 gcaatgcgag ataggtttct tcgacgtgac tgagatggaa aactactttg cgcccgaaca
     1681 gctgtgtgcg caggttggtg ggtgtatcca gggttaataa tcgggtttta aatacgccaa
     1741 ttttgtcaca cagacgatcg gcttcgtcga ggttgtgcgt gcacatgaag atcgtgcggc
     1801 cttgtttgcg cagctcttca ataaactcgc gcaccaagtg tgaggcttca gggtcgagcc
     1861 cggctgtggg ttcatccaga aacaatatac gcggttcgtg gagtatggca cgggcaattg
     1921 ccaatttctg cttcatgcct ttcgagaatg tacccacctc atcctggcgt ctgtcccaca
     1981 ggcctagcat ccgcaggtat ttctccacct ggccgcgcac atccttcact tcatacagat
     2041 ttgcataaat ctgaagattg tattcggcgc tcaggctgtc gtacaaacca ggcgattcgg
     2101 tgagaatacc taccgtttgg cgaattttcc ggtcgtcttc tccgatggtg tagccgttga
     2161 caatggcgcc tccgctggtt ggggcgatca ggctggtcag catgcggatg gtggtagtct
     2221 tccctgctcc attgggtcca aggaagccaa atacttctcc ttcacctact tccaaattga
     2281 ggcgctcaac ggcaagcgtg ccgttaaatt ctttggtcag attgtgcgtt tgaatcattc
     2341 tataaaccgt gtccggtcaa ttttttccgg tgtttcaatt atattatggt caataacttg
     2401 tgggggctcg ggttgaaaag catcgacagt ggaatcttcc atgggttgcg gctctggttt
     2461 tcgcagtaca aatatgatag tcaagctgat gagacaggca acggcgacaa ggaattcagc
     2521 tatatatact ccccgcgtac tcgcgctgaa gactaccacg ctgtctatta tggcgtgcca
     2581 gcccacggcc aggaagagcc atccaatctg cccgcgaata aaggattgca agatgagcac
     2641 gctgaagcag atctggatag gcagtgtcat agcccgctcc agagctccca acaggataag
     2701 ataccaaggc gcagaccagt aggcagcgac tgcatccttg gcgatggcaa gttgattttg
     2761 aggcaccatg gtggacaggt ctacgttgcg tatagccatt tgatagaaga agttggcaaa
     2821 tacaataata cccagcagaa ccgcctcgat cccaccatgc cccgcgccca gccagactcc
     2881 cttgcgccac gagcgtgcat cttttaccca ccagcgaaaa acggcgtagc gtgtaagttc
     2941 ttcccataag ccagcgctca atcccaagat tgtcgagttg aaaaccagct tggcggagtc
     3001 gggtggggcg gggagtatgc ctctttgaaa taggacagtc agcccgatat tgaaggggat
     3061 atgacccact tgcgaaagaa cgaacccagc tccaccgatc caccaaagtc gccatcccaa
     3121 tctgaattta cgctccaagg ctatccccaa accaaggggc agagcgatca tcagaaaagc
     3181 agatagcggg tatgcgaggg taggaggcat ggatgtgtag taaccttttc gtccccccca
     3241 catggggcat tagtacatct atgaaatagg ggtgaggtca agattattat acctgccgat
     3301 ttatttataa actaaataga ctcgctgatc gtcgcagggc aattgcatat gacctgaggg
     3361 gcatgcgcaa tcccccaaac actctcttca gtggtgggaa atcgtcctaa tgcgattgcc
     3421 ctactgatcg tcgagcttat tactcctatc ttggaggatt ctggtataat ttttgctggt
     3481 acgacgactt aatggtatct acttttaacg ccggggtttc cccggtgttc ttatgtggga
     3541 gaaaatatgg agaagaacga gtatctcgat ttgtattacc agatggtgct gatccgcatc
     3601 gtcgaggaac aagctgccca actctaccag cagggaaaaa tcgggggctt tttacaccta
     3661 tatatcggtc aggaggctgt cagcacaggc ctgatttccg tgcgccagcc acaagatcga
     3721 gttattacgg cttaccgcga tcacggtgtg gcgattaatt gcggcatttc agccaaggaa
     3781 gtcatggcag aactgctagg caaagccacc ggcatctcga aaggtaaggg cggctctatg
     3841 catatggccg atgtcaagaa aaatttctgg ggtggtcatg cgatcgtggg agctcatctc
     3901 cccctggcag cagggttggc tttaggcgat caatatcagg gtaatgatgg cgtgactatc
     3961 tgtatgtttg gcgacggtgc cacaaacatc ggttacttcc acgaagcgct caacctttca
     4021 aaagtttgga atctgcctgt gctgtgggtg tgcgagaata accagtatgg catgggcacc
     4081 gcggtagagc gcgcttcagc tgtatctgaa atccgacaga aggcagaagg atacgccatg
     4141 gcgaatggtc gcgtagatgg gatggatatt atggcggttc gcgaagcatc ccgtcaggca
     4201 cttgagcagg tgcgcagcgg tcagggacct tacttcctag aggtgatcac ctaccggtac
     4261 cgaggtcact cgatgggcga tccggagcga taccgccagt ccgatgagat cagaaagtgg
     4321 caggaaaatg atccgattgg tatatatcgc ggctatctca cgggtcagaa aattgcttct
     4381 gaagaagagc tggatcaaat cgactcgcag gtaggagacg aggtccagca ggctgttcag
     4441 ttcgctgagg caagccctga accggctccc gaagcgttgt ttgagcatgt ttatgtagaa
     4501 ggataggaat aggggataat gtcaaaaatc accatgcgag aagccatctc caaagcactt
     4561 tgggaggaga tggagcgaga ttcaaaagtg tttatcttgg gtgaagaagt gggggtttgg
     4621 ggtggcactt acgctgtaac caagggtttc gttgaccact ttgggcctga cagggtgcgc
     4681 gataccccaa tcgctgaggc ggcaattgtc ggcgcagcca tcggggctgc tctcactgga
     4741 ttacgaccgg tggctgagct catgacgatt aattttgctt tttccgctat ggatcatatc
     4801 gttaatgagg cggctaagct gcattacatg ttcggcgggc aattggtgtt gccggtggtt
     4861 atccgaacgg ttagcggcgg gggacggcaa ttaggagcta cccattctca aactccggac
     4921 gcaatttttg cccattttcc tggattaaag gtggttgccc ctggaacacc cgaggacgcc
     4981 aaagggctgt taaagtcggc cattcaaagt gatgaccctg tattgttcat cgaacatgca
     5041 acgctatacc aggttcgggg cgaggtgccg gatggcgatt acacgatccc cattgggaag
     5101 tcaaaaattc agcgacctgg ccgagatgtg accatcgtta cctacagcaa gatgcttgaa
     5161 atctcgacca aggcagctga gcagctttcc caagaaggca tcgaagttga gatcgtcgac
     5221 ctgcgctctc tgcgaccatt ggatatggag ccagtgctcg agtcattcaa gaagaccaat
     5281 cgtgccgtga ttgtggaaga aggctggcga tcttatgggg tgggttctga agtggctgcc
     5341 cgtatttatg agcaagcttt cgattatgtc gatgccccta tccaacgggt tgcccaagaa
     5401 gaagtgccct tgccatataa ccgtaatttg gaacaactgg ctttgcctca ggtggaagat
     5461 gtagttcgtg cggtgaagga ggtactctga tggcagaaac cgtcacgatg ccgaaactag
     5521 gttttgatat ggctgaaggc accctgatac gatgggtgat tgctgaaggt gaggcggtga
     5581 ataaaggggc gatcctggcg gagattgaga ccgataaggc gactgtggag gttgagtcag
     5641 tgtatgatgg catcctggcc agacaccttg tcgccgaagg cgatattgta ccggtaaata
     5701 cacccattgc aattgtcgct gcccccggag agaaagttga aaaacagcct caattggtaa
     5761 ccgaccaagc tgctccgacc ccaaaatcgg cagcggaagc cgaacctatc agcgctcctt
     5821 cccaggcgcc cagcgaacca accgtggtcg aaggacagct ccccgaaggg gtgaaggctt
     5881 cacccctggc acgccgcatg gcggaagagc ggggtatccc tttgagatcc atctcaggta
     5941 gcggtcctgg cgggaggatc acgaagaaag atgtggaggc ctacaaagct gtgcaagccg
     6001 caccaaccca acctcaagta gcctttcaag ccggcttacc ctcgattcct tcaaccacca
     6061 ttccgttgaa gcgcttgcga gccatcattg ggcggcgtat gacagaagcc aaacaaagcg
     6121 ttccgcattt ttacgtgaca catgaatacg atatggcagc tttactggca ttacgccagc
     6181 aggtcaacct gactctgcct gacgaagaga aaacctctgt aaacgacttc attcttaaag
     6241 cttctgcgct tacccttagg gaatttccca atctgaatgc ttcgctggac gataacaata
     6301 accagatcat ccaacatggg gcagtaaata tcggtgtggc ggttgccgtc gaaggcggtt
     6361 tgctgaccgt ggtttgccgg gatgctgatc gcaaaccaat ccggcagata tctgccgaag
     6421 tgcgtcagat ggtgcagcgt gcccgagaag gcaaggtgcg cccagatgat atcgaagggt
     6481 ctacattctc ggttagcaat atgggaatgt atgacgtgga gcactttatc gccatcatca
     6541 atccccctga agcagccatc ttggccgtag gatcggcgcg tgaggtgccg gtggtgaaag
     6601 atggggaagt tgtgcccgga ttgcgcatga aagcaactct ctcggtggat catcgcgtca
     6661 gcgatggagc tgaaggtgct cgcttcatgc agaccttggc aaggtatatc gaagagccca
     6721 tccggttatt actttagaaa agagcaactc cctacaaaaa cttgtaggga gttgtttctt
     6781 gtatagcttc attcattgta gcacttactt gaatgcgtac acgactgatt cctcatcgcg
     6841 gtatacctct tccagaccgg ggtcctcttc ggctctgcgg atgaagtctt tatggtttaa
     6901 atcgctcacc acgaaattgg cgccaaagcg atcgtaaata aattgggaag gtcgttcgac
     6961 atctccttcg gtgatctttt cccagagatc atacaaatcg gcgttgtaca attgcatata
     7021 ggtgggatct aggccgatca aataggtatt gtgcgtgtta tggtagaaga gacgaggaaa
     7081 atcatcccaa tctgtctgaa atacacgcga gccagatggt gtgttggctt tcaaccattc
     7141 ggatgcccca gaaaaaagtg tgtatggttt agaagatttt acgctctttt gcgcgtcgcg
     7201 cacggtcagc catatcccag ggattagtaa ggcgaccagt acaacgactg cggtcacctg
     7261 gcgccagcgt aatcctaaaa tggaatattt cagaaagcgg aggcttggat cggatacatt
     7321 aatgtcatcc tggttggtgt cttggagttg caagagggga ctccaagcaa atgctgcgaa
     7381 tatcaaggcg aatggaggga aatattcaat aaatcgccgc gattgaagga gcaataaacc
     7441 aaaaaggagg gcgatgaaga ggctcgttgc agtgcgtgtt tccatgcgtc gttctcgcaa
     7501 gcccaaagca agcaccccgg aaaagaaagc gaccagtgtc agtggggaat tctccagcaa
     7561 ctggctggtt tgatagggat accattcgtt gccgacatct aaggcagtcg tctccgtgat
     7621 tttgggtgcg aggtgacgaa tggcgaatat gatgtcgttt ggaaaatatg gattaagaac
     7681 caaccccagg gttaccccta ctccacaata gatcaggggg cgcagctcca agcggcgttc
     7741 gatgagtagg atcgaagcga cgtaaatggt ggttagtgtt aataaaaggg gaaatgcatc
     7801 gtatagccag acgtacacga atcccagggg aatcaaacga acgtattttt tcgtcagcat
     7861 ccagtgtaga gcgaccgcca tgacagccaa ggtgagtgat tgtctcctta ccaggctcat
     7921 gcgaaaaata aaagcctccg aaattgctag caatcccagc gcccacagcg cgctataggg
     7981 gacacgctgc actcgcagca accaccaaat gcacagaaaa gctatggcag ggaagagcac
     8041 agcagctaat ttggctcctt ctcgtaagtc acccaatgtg aagggtatta atgcaatgtg
     8101 aaagagaaga tggtggtcgc tatattcgcg cgggttgaga attgtaagcg gtagccaagg
     8161 gaaaccgggt tttagtccct ccgttcgcat gatgctcgca aagcgtatgt ggaaatagcc
     8221 atctactcct gcgagattgt ctgtggagaa ttgcactatg cttagaaggg cgataaaaat
     8281 gagggtcatc ccaataatgg atagaaaagt ccgcacctca ggatgatcca gcgtaaatct
     8341 tacaattcga acacgtggtt taatctgttc tctcatctca aatttggatg tgtttttctc
     8401 ggataggtct cctgtcatca gatctccgct agaacaaaaa acctccgagg ttaaacgcat
     8461 gtcgatgcga ttaatggcct cggaggtctg aagatggttt taggcattaa tcatcgtcgt
     8521 tgctattacc accagaacca ctgttatcgt tgctcccacc actcgatcca ctgttatcgt
     8581 tgctatttcc tccagagcca ctattatcgt tactgttacc acccgatccc ccactgttat
     8641 cgttattatt cgtgttggtt tgggaaccgc tacctgaggt gccgcttcca gtgttgtcat
     8701 tcatgttctg gtttccgata ccgtgctgca agccttcatt ttcgttgctc gcattcattg
     8761 tctcattctc attttcgatc tcgttcacgt tgagatttgc agttcccaag ttgtcattag
     8821 cattggtgat atcgtcgctc tcgttggtat tttcattccc gttcaagttc tcgtcacttg
     8881 ccagggtcac ctcgatctcg cgcgctgtca aggtgccatc agcagcctga gtggcgtgta
     8941 ccttgaccat tgcgcctatg tctaggctgc ctttaatttc cgtttcgggt gtgatcacta
     9001 ccagctgacc agcgatgcga taaccctcat cagatatgct ttcgatgaga cccgtgaatt
     9061 caatttcgtc ctcgtgctct tcttgctcgg atatcaggat tgctttttca acagatggct
     9121 tgatggtgtc tggaactgag atcagcatac cttttagaat ctcggcatat cgggtgagcg
     9181 ctgaagtgat ttggattgct agttcgccag ctcgctgggg atccccagcc gagacagttt
     9241 cgagggcgcg aatggcttct tggacgtgtg tttcaaactc acgggtggca gtttcaatgt
     9301 gatcaaaatc cccttcagcg atcagggatg cgatttcgac cagtcggcgt tcggcaaacg
     9361 ataagtggag ctcagcctgc cttgcagcat cacgagctaa cctgacctgg gttagttcca
     9421 ggctagtttt taccggatag agggcatcgc caggaagggc accttgggag gcgtatgctg
     9481 tagcacctgc acctccgaag agtagcaaga taatggcgag aactgccatg aaggttgtaa
     9541 aagccagcct ttgctgcagt gaagccaggc tgaagcccca ttttgaccgg ctttccttcg
     9601 gtgctgatat acctgcctgg tgaattaccg gacgtgtatc ctcgtcaaaa agcgcatcca
     9661 attgagataa atattttgcc cgtccgcgag cttcggcctc tgggtttctt gcaggggtct
     9721 cgcgaagcga atccaataat tctttcaatc ggggatcgat gggttgttcg tgatcgttta
     9781 gtgtagtcat tattctttca tgcctcattt tcaattagaa ggcgtctaag tgcggctagc
     9841 gcccggtatt gtaaggcgcg ggttgcttcg acagtcttgc cgagaacagc ggagacctct
     9901 tcgtgagacc agtcctcaaa tactctcaac ataataactc tccgctgctc tggcggtaat
     9961 tggagcatcg ctcgtcggac acgctcttgc tcaatatttt ttgtcatcag ttgcataggg
    10021 ttagtgtcat tgtcagcaag cagatcttcc tcaagcgata gcggaggagg tggctggcgt
    10081 cggtagtgat ctgtaatata gttatgagcc acccggtaaa gatatgcctg cacattttca
    10141 ctcggcccgc ctccgtttcg aatagcctgg atgaaacgac taaaggtttc agagacgcaa
    10201 tcctcagcca gatcctggtc gcctaagagg cgataggcat atcgatatat ccccggactg
    10261 tattgctcat aagaattaat catatcttgt ttagtcaata tcttggcttg aggcctttga
    10321 gtggtatcaa tttgcatgtc tttcactcat tatgacgaag aaagtggaat tatgtgacga
    10381 atttgataat aattgtttgg gattatccaa tctcttatta ccttactata tacgtgtaat
    10441 tatatcgcaa cagatttgtc ttcccgtttg catccttgat gaaggttatc atgtggtgtg
    10501 tcagttaggt gaagcgagct agattgcagg gagcgtagca gcttagtcgc ttggttgggt
    10561 gatgggggta taatttaatc acaaccaaat ggctataacc attgagtctg atcggtaaat
    10621 tgacatggta gccgtgttgg aggtgaactt tgttaggggg agcatgtcga atgaaaggaa
    10681 gggagagtgc caggtcctgc ctatgagata agctatttac aaaacacact agagttgttg
    10741 gagaagtatg ttttgtcagc agacatctac tggccggttc gctctgctcc ggaagcgggt
    10801 gaattgccat tcccaagtct taccctgggc ggggtgctac tatctctggc ccggttaaac
    10861 tcaacaacga tctcagatca acatcaagcc atgcgagagc aacttcgtgg agatatcgag
    10921 tccatagcta tccgctggcg ggttgcatgg gagagcaaag cagtgaggga attccacgcc
    10981 cgattgatac tttggagaga ctatttagac gagtatcgct ccaacccaga gaacaacacc
    11041 gaccgctact cttacgaagt gaatcgtcgg gttatgttgc atctgctaga gccgtatgta
    11101 gaacatgctc ctgaagctga aaaagagatg ttacgcggcc ttgaccagct attaaaggca
    11161 acctttcaag ctggggagtt catttgggat gcttggttgg aatctgcttt cccacctcaa
    11221 ccatactggt atctattcgg taaattgagg ggatctaagt gacatttata cgctggtcgc
    11281 ctgctccagt tggctccacg ccggttcaac gtaagaattt tattaatgtc cagatcgatg
    11341 cgattggcat tggcttggca agtgctctgg caccgttctt acctgtgttt ctaacccgcc
    11401 tgggtgccag tagtgttcag gtgggtcttc tgacctccat gcctgcattc actggtttgc
    11461 tcctagcaat tcccttgggg cgttttctgc aaacccgtcg tcaaatagtg ccctggttcg
    11521 gtgcagcccg cttgatggta gtctcgagct atgccatgac cggcttgatt accttctttg
    11581 tgccagagaa atacttagta aattcaatat tgctgatatg ggcggcagcc acgttgccac
    11641 aaaccattgt ggcggtgtgt ttttctgttg tgatgaacgc tgttgctggc ccacagcatc
    11701 gctatgagct gatgagtagg cgttggtcta ttttgggagt gacaactgct ttgaccgtgg
    11761 cgttagtggg gcagatgctg aatcggattg aattcccctt gaattaccaa ttcgcctttc
    11821 taaccctctc aattgggggc ttaattagct tctattattc aagtcatatt cgcttgccag
    11881 atgcagtatt agaagaaact gctcggggga tatcttggcg agaccgtttt cgtgattaca
    11941 tacggctcat taccagcgag aaggctttcg taaatttttc attgaagcgc ttgtttttta
    12001 tgttcggctt cacgcttgct gtgccattgt atcccttgta ttatgtgcgc gaagttcatg
    12061 cgaatgatgc ctggataggc ataatcagca cggtccagtc ggctgtattg ctggttggtt
    12121 atttcctatg gacacggggg tcgcgactgc gaggttcccg ttttgtgttg ctaaggacta
    12181 ctttcggttt agccgtctac ccagcacttg ttgcgctcac gactcgggtg gagttgattg
    12241 ccctgttcgc agggcttgcg ggaattttcc aggctggtct cgaccttgtt ttttttgacg
    12301 agctaatgaa gaccgtgcca ccacaatata gtgcgacgtt cgtgtcgctt gcgcaaagct
    12361 ttcaacatct tgcggctatg ttgggaccta tgttgggaac cttacttgct gatcattttg
    12421 ggctgggcat tgggttgttt gtcagcgcaa tcctgcggct attagggttt atacttttct
    12481 cgcgtagatg atgggttatg agtggttcac agtgtaagag ttttattaga aacttgactt
    12541 aatgtggaca ataacttgac ataataggta taatttattt aggttagtct ctaatgacat
    12601 gttatattaa gaggctagga ggataaaatg gatgccagtt tgagactggg taaaatttgg
    12661 gaaataccaa tagggctgca tacaagctgg ttcttggtat tcggcttgct gacatggtct
    12721 ctggcaagcg gttacttccc tgaggagtac ccgaatctct cgagcattgt ctatatcatt
    12781 ttagcgttgg tcaccagcct tctgtttttt ggctctgtgc ttgctcacga gcttggtcat
    12841 tccttcgtgg ctatccgtaa ccggatacca gtcaaaggaa ttacactatt catctttggg
    12901 ggagtggcgc agataagtaa ggagcctggt tccccgggcg aggaatttcg cattgccatc
    12961 gccgggccaa tcgtcagcct gttattggcg ggtgcgtttg gagtagtctg gctgtttgac
    13021 cagagcatac cgatcttggc cgcgccgagc atgtacctat tgcgaatcaa ttttatgctg
    13081 gctgccttca atatgatccc aggatttccc ctggatggtg gacgtgttct gagagcaatt
    13141 atctggcgta ttaatggaaa ctttcaaaaa gctacccaaa ctgcctcgta cgcgggacaa
    13201 ctggtcgcat ttggttttat tggttgggga gtattcacca ttttcaatgg tcaatttctc
    13261 aatggattgt ggctggcctt tatcgggtgg tttttgcaga atgcagccgc tactgcctat
    13321 gctcaaacaa acatgcagag cacacttcgt tccatccatg tctcgcaggt catggaacga
    13381 gattgcccta gggtgccaag gttgctcacc atcaaccaat tagtggagga tcacatatta
    13441 acgggtggtc aacgctgttt ctttgttgct gatgacgaga ggctattggg gatgttgact
    13501 ctgcgagata tcacccagat cccccgccct aagtggcgat tcacgaccac tgaacaggcg
    13561 atgatcccgc taaggaattt agtcagcgtt gaaccgaata cgcagttgat gagcgcttta
    13621 caaacgatgg ataatgcgaa aatcaatcag ctgcccgttt tgattggaga ggagcttgtt
    13681 gggatgcttt ctcgtgagca gattgtccac tacctacgta cacgtacaga attaggggtt
    13741 tgatatccga tggaagcgac accatttctt caactcatta ttgccttagg ggtgattata
    13801 gtagccgcta agttaggtgg ttacgcaagc tactctctgg gtcagccttc cgtactaggg
    13861 gaattgttgg ttggaattat tattggacct tccttgctta acttactcca cttgccatat
    13921 ttttctgacg agcatctggt agaaaccgtt gctcatctag gtgagattgg tgtacttctc
    13981 ttgatgttta tcgcggggtt agaactacat ttggatgaac tggccaaaac tggcaaggta
    14041 gcagtttttg caggtatcct gggggttgtg gtgccgctgg ttcttggtac aggattagga
    14101 ttattgttcg aatttgacat agcacctgct gtcctggttg ggttggtatt atcagcaacc
    14161 agtgttagta tctccgcgca aaccctgatg gaacttaagg tgctacgcag ccgagttggg
    14221 atcggcatga tgggatcagc ggtattcgat gatattttgg tgattctggg gttgtcgttg
    14281 ttattcgccg tcacccactc cggagggaat gctagcattt tcggtatcct gtggatcgct
    14341 ttacgtatgg ttctttatct ggttggagcc tggttggtgg gtgcgtggct gttacctaag
    14401 ttgggggcct gggtggaaaa gctgccgatc agtatgggtt tgatcagttt cgcattcgtc
    14461 atcatgctgg tttacgcctg ggctgctgag acattaggtg gcatggcagc aattacgggt
    14521 gcatttttag cgggtttgct gctggcacgt actcggctca aagaacgcat cgatacaggc
    14581 atagctacaa tatcctatgc catctttgtg ccgatcttct ttgtaaatgt tggattatct
    14641 gcgaatgccc gattattgac tgtagatact gcctggtttt tcattttgat aaccatatta
    14701 gctatcattg gtaaggtgct tggcgctggg ttaggcgcca acttggcggg attttcccgc
    14761 ctggaagcac tccagcttgg tgtagggatg atgtcacgag gagaggtagg gttaattgtt
    14821 gctacggtag gaattgctga aggcttgctg ttcccggagg cgtttgcttc gattgttggc
    14881 atggtcatcg ttaccacgtt gatcaccccc ccgctcctac gagtattgtt tacccgcccg
    14941 ctcaataaga agcccagcga ggcttagttt cggaggacat taaacaatgg catacttaat
    15001 tgtattgatt attgatgatc cagatgactg cccagcaata cttgaagggt gggaggcgat
    15061 tggcatcggt ggaataacca ttctagaaag cagcggtttg ggtcgcttcc gccgcgcggg
    15121 aatgcttgac aatttgcctt tgatgcccag tatgcgggat cttttcgaga aaggagagac
    15181 ccatcatcgc acgcttcttt ctgttgttga agaccaggag aaggtcgacc agatggtc
//
LOCUS       DBNGSX010000141         3050 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1534547, whole genome shotgun sequence.
ACCESSION   DBNGSX010000141 DBNGSX010000000
VERSION     DBNGSX010000141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1534547"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="AC16CB_04715"
     CDS             complement(<1..360)
                     /locus_tag="AC16CB_04715"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HIR3341805.1"
                     /translation="MPCYIDDHQRLEATLRYNIQSNITAIFILIAGVGWIWLSKAFPD
                     DLSVDGKIAPRAGFLAPDFSLLDRDSNQITLSELRGRPVIINLWASWCIPCREEMPAL
                     EQVHQAYKDQGLIVLAVN"
     gene            612..1472
                     /locus_tag="AC16CB_04720"
     CDS             612..1472
                     /locus_tag="AC16CB_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012314.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR3341806.1"
                     /translation="MKISVCLVFVSLLLLTACRGPETLEPVVTSQVSITPLGETGQLL
                     TPSPVDQKPLGGAYPEPEVTMPSQSLQSTSVATENETYPPPGSTSVTGGSDAYPPPGT
                     LATTTGQELGNFNQPYPAPETDIPLGSTGTSQSQGISGQTAVSTPTTTLIPTSNPTIT
                     SISTATPTPTAPMGLVRTELRASDPSEFSLVSGELQLVMFFAHWSPLSKSMAPVMNAL
                     EEKYQDRITFFYLDIDDPANNFYKYLLGDRLPPVFFLIDPQGIVLNEWQGFVMPEEFE
                     AAFASVVITP"
     gene            1635..1958
                     /locus_tag="AC16CB_04725"
     CDS             1635..1958
                     /locus_tag="AC16CB_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341807.1"
                     /translation="MRNKLKYVWVNLFVFLLVALMAGCGRATQPEATNPVETPTASQT
                     VVIQPTPSATEASAPTQTPTTEIIIESPTAPPPTPRPGLEASEPSSFVQASGQYQLVE
                     FFAFW"
     gene            2341..2610
                     /locus_tag="AC16CB_04730"
     CDS             2341..2610
                     /locus_tag="AC16CB_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HIR3341808.1"
                     /translation="MKHDNVSLRLKNVEGHIRGIRRMADQDAYCIDIIRQIQAVQAAL
                     NKISTAILEQHLNSCLTTAVRGDDPDERERVLNEIASVFETATKT"
     gene            2631..2837
                     /locus_tag="AC16CB_04735"
     CDS             2631..2837
                     /locus_tag="AC16CB_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR3341809.1"
                     /translation="MTTVTYSIPNISCGHCVQTIQNEISELDGVISVIANRDMRTATI
                     MFEPPATEEKIQSLLAEIDYPVVA"
     gene            2840..>3050
                     /locus_tag="AC16CB_04740"
     CDS             2840..>3050
                     /locus_tag="AC16CB_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HIR3341810.1"
                     /translation="MSETKHVTLPVTGMTCANCVSTVERNLKKVQGVQQASVNLSSER
                     ASVEYDPSLANLGDLIARVQKAGYGV"
BASE COUNT          829 a          728 c          703 g          790 t
ORIGIN      
        1 atttactgcc agtacgatca gaccttggtc tttatatgct tgatgtactt gttcgagggc
       61 aggcatttcc tcgcggcatg gaatgcacca acttgcccat aggttgatga tcactggccg
      121 gccgcgaagc tccgataaag ttatttgatt actgtctcga tccagaaggc taaaatccgg
      181 cgccagaaag cccgcacgcg gcgcaatctt gccatcgacc gataaatcat cagggaatgc
      241 tttactcagc caaatccagc ctactcctgc gatcagaatg aatatagcgg ttatattcga
      301 ttggatgtta taccgcaatg ttgcctccaa acgctgatgg tcatcaatat aacatggcat
      361 aaagatctca tcaatggcga tgatctttgt aagaccgttt ggacaccggt tgtggttata
      421 tctcgtgcgg caatttcacg ccgtgcaggt aatggagact ccccagcctg cttgggtaat
      481 cacgttgtat atctttgaga taacatgttg gaatgtatac ggatcttgat ccaattctga
      541 ggttactggg cttgacatat tattgatcaa tgatatgctt tggtcagtta gtttatgggt
      601 ctggaattta tatgaaaatc tctgtatgtc ttgtgtttgt aagcttgctt ttgctgacgg
      661 catgccgtgg accggaaacg cttgaaccag tggtcacttc tcaagtgagc ataactccac
      721 tcggtgagac aggtcaacta cttaccccaa gccctgttga tcaaaagcct ctaggcggag
      781 cctatccgga gcccgaagtt accatgcctt cgcaatcgct gcaaagcacc tctgttgcta
      841 ccgagaatga gacttatcca cctccaggaa gtacctctgt tactggggga agtgatgctt
      901 acccgccccc aggtacttta gcaacaacca ctggacaaga attgggcaat tttaatcaac
      961 cttatcctgc tcctgagaca gatattccat tgggtagcac aggtacaagt cagtcacagg
     1021 gaattagtgg acagaccgcg gtgtcgacac ccacaaccac gctaattccc acatcaaacc
     1081 cgacaatcac atcaatatcc acagcgacac caacccccac agcgccgatg gggctagtgc
     1141 gcactgaatt aagagcctca gaccctagcg agtttagcct cgtttctggg gagcttcaac
     1201 tagtaatgtt ttttgcacat tggtcaccgc ttagtaaaag catggctccc gtaatgaacg
     1261 ctctggaaga gaagtatcaa gatcgaatca ctttttttta tctggatatc gacgatccag
     1321 ccaataactt ctacaaatat ctacttgggg atcgtttacc gcctgtcttt ttcctgatag
     1381 accctcaggg gattgtctta aacgaatggc aagggtttgt gatgccagag gaatttgaag
     1441 ctgcttttgc ctcggtggtt attacaccct gatacccaaa actgtaatat ttatgtaatc
     1501 attgcattat tgacaatcaa gggggaaatg gtaagattat tctcctagat gttatccaaa
     1561 agaaaggggc gcatgcaggt ggaagattaa catcgctgcg taatatttgg aaaaacacac
     1621 gaggataatt ggcattgaga aataaactaa agtatgtatg ggtaaacctt tttgtcttct
     1681 tgctggtagc gctcatggca ggctgcggac gagcgacaca acctgaagcg acgaaccctg
     1741 ttgaaacccc aacggcttca caaactgtgg ttattcaacc gactccttct gcgactgagg
     1801 caagtgcacc gacgcaaaca ccaaccacag aaataataat tgaaagtccc acggcaccac
     1861 cgcccacacc acgtcctggg ctagaagcgt ctgagcctag ctcattcgtg caggcctcag
     1921 gtcagtatca actggttgag ttttttgcct tctggtgaac gacctgccag cgaatggcgc
     1981 ccatcgtgca tgggttggaa gccaaatatt ctaaccgcat caagtttatt tatcttgata
     2041 tagacgaccc agctaatcaa caagccatga aggatttagg atatatcggg agaccacatt
     2101 tctttctcct agataccgat ggaaatgtac tagggcagtg gctcggatac gttccggtgg
     2161 aggatttcga aatagcgttt gcccaaataa aagaataaag gtgttggttc tactggattt
     2221 gctaagacct ggcaatagat tggcgtttcc aggtcttttt tcttgacaag atatggctat
     2281 gtgatacaat aaccataccc ccccaggggg ggtgggatac ttaggaacaa ggatagtgct
     2341 atgaaacatg ataatgtctc gctacgtctc aaaaatgttg aaggtcacat tcgcggtatc
     2401 cgtcggatgg ctgaccaaga cgcatactgt attgacatta tccggcaaat ccaagctgtc
     2461 caagctgcgc tcaataaaat cagcactgca attcttgaac aacacctaaa ttcttgcctt
     2521 actacggccg ttcgcggcga tgatcccgat gagcgggagc gcgttctaaa tgaaatcgcc
     2581 agtgtttttg aaacagcaac gaaaacctaa atctaactaa ggagataccc atgactactg
     2641 taacctattc cattccaaac atttcttgcg gtcattgcgt gcagactatc cagaacgaga
     2701 tcagcgaact ggatggtgtg atctctgtga tcgcaaatcg ggatatgcgt acagcgacaa
     2761 ttatgttcga gccccctgcc acagaggaaa aaattcaatc actcttggcg gagattgatt
     2821 accctgttgt agcttgatca tgagcgaaac caagcacgtc acactaccgg ttaccggtat
     2881 gacttgtgcc aactgtgtct cgacggttga gcgtaatctc aaaaaggtac aaggcgtcca
     2941 acaggcatcc gtcaatctgt cttctgagcg cgcgagcgtt gagtatgatc cttcgctagc
     3001 gaaccttgga gacctcattg cccgcgtcca aaaagcaggc tacggggttg
//
LOCUS       DBNGSX010000142         2283 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1119178, whole genome shotgun sequence.
ACCESSION   DBNGSX010000142 DBNGSX010000000
VERSION     DBNGSX010000142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1119178"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="AC16CB_04745"
     CDS             complement(<1..528)
                     /locus_tag="AC16CB_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HIR3341811.1"
                     /translation="MSSSADHNHSISSATENYLESVALARSQNTARTYRNALSFFLQV
                     LAQHNHSPKYDPISELPEDAISWFAAALKGYAPATERLYLTAAIGFFEYLAAERLADV
                     NLPRVRLLVRQRARRPGQRLPQFSRNSIEDVVKYAINIANLPTDTEPERLRNLRDRAF
                     LITLADSGLRVHEACG"
     gene            1018..2208
                     /locus_tag="AC16CB_04750"
     CDS             1018..2208
                     /locus_tag="AC16CB_04750"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HIR3341812.1"
                     /translation="MPIPTPFHSRTAPLCKSHEWRTWSGYLAASLYQINHDAEYYAIR
                     NAVALIDVSPLFKYEIRGPDSVRLLNRVMTRDIAKCVVGQVMYTPWCNDEGKVIDDGT
                     VAHLEQNLFRVTAADPNLVWLQDNGIGMDAEVTDVSSDLAALAVQGPNSRQVLKHLLP
                     KSDLDALRYYRLIHAKTGRINLTVTRTGYTGDLGYELWMDPKHADTVWDMLMEIGVKY
                     GMMPAGMVALDIARIEAGLLLIDVDYISSRKAHIPDQKSSPFELGLDWTVDLKKENFI
                     GRAALMEEKRRGSKWQFTGIEVNWKSLEALFSEADLPPLVAGRASRTSLPVYKHNRQI
                     GYASSHTFSPIMKKYIALASLNYPLAKPGDSVEIEITVEHVRHRAKAVITDLPFYNPV
                     RKRA"
BASE COUNT          612 a          512 c          549 g          610 t
ORIGIN      
        1 accacaggct tcgtgtactc gtaggccgct atctgccaga gtgatcagga aagcgcgatc
       61 ccgcaagttc cttagtctct caggctcggt atcagttggt aaattagcaa tattaattgc
      121 atatttaact acatcctcaa tggaattgcg tgagaattgc ggcaaacgct ggcctgggcg
      181 gcgggctcgc tggcgtacca gcaggcgaac acggggtaga ttgacgtcag ccagacgctc
      241 ggctgccagg tactcaaaga aaccgatcgc agcggtcaag tacaggcgct cagtggcggg
      301 tgcgtaacct ttgagtgcgg ctgcaaacca ggaaatggca tcctctggta gctcagatat
      361 tggatcgtac ttgggggagt ggttgtgctg agcaagaacc tgtagaaaga atgacagtgc
      421 attacggtag gtacgtgcag tgttttgact tcgtgcaagc gccacgctct ccaggtaatt
      481 ctcagttgca gaggaaatag aatggttgtg atcagcagaa gagctcatga tgcgtgtttt
      541 tcctgaaata ataatgttta tgataataat acttatcaaa agcataaata ttatagcaag
      601 gaatttttgt ttgtcaaccc ccctgctcta gtgttccaaa caattctcgc ggaattggtc
      661 tcaccaaagc caattggcgg taaacaatga gtaaatacac cgcggcttat ttctactacc
      721 cagaccttct cgcaaaacct cccccctctt tgtaagatct ctgtttgctt ttgtgggatg
      781 ttttttgtat tacaattaga aaccctgaat tgaagagtcg aatgcatttt ctgggatcgc
      841 agctctattg cttaaaatac aagtatatat gcgtaatata tatagagcac ataattaatt
      901 gaaataatca cataatactt gcgctaagta aagggctgga cgtatattaa ataatgcatt
      961 aaattacgtt gaaaatcgtt cccgagataa aatatttcaa tcctcacaga atagtctatg
     1021 cctatcccaa cacccttcca ctcccgcact gcgccattat gcaaaagcca cgaatggcgc
     1081 acttggtcgg gctatttggc tgcgtcgctt tatcagatta atcacgacgc cgagtattat
     1141 gccattcgta acgcagtggc tttaatagat gtttcgcctt tattcaagta tgaaattcgc
     1201 ggtcctgatt ctgttcgctt acttaatcgc gtgatgacca gggatatcgc caagtgtgtg
     1261 gttggtcagg taatgtatac cccttggtgc aacgatgaag gtaaagtaat tgacgatggc
     1321 actgtggctc atctagagca gaacctgttt cgcgttaccg ctgcagaccc aaacctagta
     1381 tggcttcagg acaacggtat cggtatggat gccgaagtaa ctgatgtttc atctgaccta
     1441 gcagctctgg ctgtgcaagg tccaaattcg cggcaagtcc tgaagcacct actccccaag
     1501 tccgatctgg atgccttgcg ttactaccgt ttgatccatg cgaagacagg caggattaat
     1561 ctaaccgtta cacgaactgg atatacaggc gatctaggtt atgagctttg gatggatccc
     1621 aaacacgcag atactgtgtg ggacatgctc atggagattg gggtgaagta tggcatgatg
     1681 ccggcgggta tggtcgcctt agacattgcc cgaatagagg ccggcctttt actgattgat
     1741 gtggactaca tttcgtcacg gaaagcccac attccagatc aaaaatcttc tccttttgag
     1801 cttggcttgg actggacagt agacctcaag aaggaaaatt ttataggccg tgctgccttg
     1861 atggaagaaa aacggcgtgg ctcgaaatgg cagttcacag gaattgaagt aaattggaaa
     1921 tcgcttgaag ccttgttctc ggaggcggat ttgccaccat tagttgctgg tcgcgcttcg
     1981 cgtacatcct tgccggttta taagcataat cgccaaattg gctacgcctc gagccacacc
     2041 ttctccccta ttatgaagaa atacatcgcc ctggctagct tgaattatcc attagcaaaa
     2101 ccgggtgata gtgtagagat tgagatcact gtggaacacg ttcgtcacag agccaaagct
     2161 gttatcaccg acctgccctt ctacaatcct gttaggaaaa gagcctgaga acatgccgga
     2221 tttaagccta tacgaaaact acgatgtaat tgtgatcggt gccggtcaca atgggctggt
     2281 aac
//
LOCUS       DBNGSX010000143         6860 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_149781, whole genome shotgun sequence.
ACCESSION   DBNGSX010000143 DBNGSX010000000
VERSION     DBNGSX010000143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6860)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6860)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6860
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_149781"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..733
                     /locus_tag="AC16CB_04755"
     CDS             248..733
                     /locus_tag="AC16CB_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341813.1"
                     /translation="MTISNRIILVVALLTIVFGGVTLAFASGKDTIYAAEVGPEIEGT
                     WLATITIPDGPPPFPSLVTYARGGALTVTDSSGSPALGNVYQGSWAKTGPHKYVFTVM
                     GFLYDEEGVFAGYIRLHEIVKIEPGGNVYNGEASIEILDPEQNVVMTGSSISYAERIN
                     P"
     gene            1062..3257
                     /locus_tag="AC16CB_04760"
     CDS             1062..3257
                     /locus_tag="AC16CB_04760"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR3341814.1"
                     /translation="MDILAGLNPQQQQAVMAGLGPVLVLAGPGSGKTRVLTHRIGYLL
                     GAMGVRPYQILAVTFTNKAAREMQSRLTQLLEARPGGSPQSADSPQMGTFHGACARIL
                     RREAEHLPFQSNFVIFDADDQLNVVKTAIKDLGLDDKLYRPPGVHAAISNAKNELLLP
                     DDYPVQTYRDEVVARVYRRYQELLLASNALDFDDLLLWMTYLLDNEPAVRDKYARRYE
                     HVLVDEFQDTNMAQYTLLKHLASFHRNLYVVGDVDQSIYGWRGADYRNVLRFEKDYPD
                     AQVVLLEQNYRSTQIILDAAMGVIDRNPHRTPRRLFTERGSGQKISLYEAYDDRGEAA
                     FVVDTIASLVGSRRAQPGDFAVMYRTNAQSRVLEEAFLHAGLPYKLVGAQRFYGRREI
                     KDVISYLRLVHNPNDEISLTRVINTPARGVGNKTVLALRTLAKKQGLSPGVLLLELGR
                     DPDAAGIEALPGRSAGPLANFGRNLVGWRDFANIATPLSLMDRILSDIDYQSYLDDGS
                     DEGRDRWDNVMELRRLAAEFQENGLEAFLEQVALVSDQDTLEERINVPTLLTLHAAKG
                     LEFPVVFIVGMNDGTLPHSRSFDDPEAMQEERRLLYVGITRAKDRLYLVYAQNRSLYG
                     YSEPIEPSRYLDDIPVDLVDETQPTRSGRLHRPAPRTDRWQPQKASVQSSDQPRFHPG
                     LRVMHNVWGEGMVLNSRLQDDDEIVDIFFESTGLKRVAASLAKLEVMEK"
     gene            3297..3794
                     /gene="tsaE"
                     /locus_tag="AC16CB_04765"
     CDS             3297..3794
                     /gene="tsaE"
                     /locus_tag="AC16CB_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR3341815.1"
                     /translation="MPILDPHSFEFISRSAEQTRRLGMRLGALLRAGDVICLVGDLGS
                     GKTTLVQGIASGWGSLDLVTSPTFVLVNVYSGPDAARLYHLDAYRLSSAREAEDLDLE
                     PMLETGLLVVEWAERIQEALPREHLWVTLRWIDNEQRDLVFAARGKRYQSVLASVRKQ
                     IFGIM"
     gene            3794..4462
                     /gene="tsaB"
                     /locus_tag="AC16CB_04770"
     CDS             3794..4462
                     /gene="tsaB"
                     /locus_tag="AC16CB_04770"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR3341816.1"
                     /translation="MLLALDTSTRVVGLALYDGVQVLGETVWLSRDYHTVELAPAVAN
                     MLARSDVKISDLSALAVAIGPGSFTGLRIGLALAKGLALAHSLPLVGVPSLDILAAAQ
                     PIQEEVMAAVLRAGRGRLAVGWYQASDGGWRAKGQVEALTAQELNERIQEPTLVCGEL
                     EEDEQRLLARKRKNVILAPVAQSVRRPAWLAELGWKRWLAGRVDDPNTLSPIYLHYNE
                     PIPG"
     gene            4459..5016
                     /gene="rimI"
                     /locus_tag="AC16CB_04775"
     CDS             4459..5016
                     /gene="rimI"
                     /locus_tag="AC16CB_04775"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIR3341817.1"
                     /translation="MMAVRSKTSPIDPPQRSNLIHIRPMRQDDLDQVITIDRASFSLP
                     WPESSYRYELLENPHSLLMVAEAEAPDGEKQVVGAVVVWLLEDEAHIATLAIHPEYRG
                     RGISQELLATVLARSIRGGARIATLEVRANNQIAHALYRRFGFEVVGRRLRYYLDNNE
                     DALIMTADGLDERYLEWLESHGWRK"
     gene            5102..5731
                     /locus_tag="AC16CB_04780"
     CDS             5102..5731
                     /locus_tag="AC16CB_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HIR3341818.1"
                     /translation="MNSKEILAQVSQEVSACTQCVLHHSRKNAVPGEGPANAKIMFIG
                     EGPGFHENEQGRPFVGAAGKFLEDLLGKIGMQRTDVYITNVVKCRPPANRDPMAEELE
                     ACNEYLERQIQAINPRVIVTLGRFSMARFLSNAKISDVHGQAMRVRGRLIVPMYHPAA
                     ALHQGSLRPVIERDFALLPELIASAADAPEVEEPKPPEEKTQPRQLSLF"
     gene            5743..>6860
                     /locus_tag="AC16CB_04785"
     CDS             5743..>6860
                     /locus_tag="AC16CB_04785"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR3341819.1"
                     /translation="MSANIKHNLIDQIKERKAQVAILGLGYVGLPLAAVMAEAGFHVV
                     GVDPDQRKVEMISRGESHVKDVPSEQVARLVKAGQLVATTDFSVLQNAQAVSICVPTP
                     LRKTGDPDLSFILDATAELARYIHPGMVVVLESTTYPGTTREILLPHLGNKKGLVVGE
                     NFFLAFSPERVDPGRKDWTTLNTPKVVGGITADCGEVASIWYSQALQSVVPVSSTEVA
                     EMAKLLENTFRMINIGLVNEMALMCNRLGVDVWEVIDAAATKPFGFMKFTPGPGLGGH
                     CIPIDPLYLSWKLRALKYTARFIELASEINTAMPRHVVDLVQDALNGQSKALKGSRVL
                     VLGAAYKPDIDDLRESPALDVIGLLEQKGALVSYNDPY"
BASE COUNT         1477 a         1796 c         2026 g         1561 t
ORIGIN      
        1 aattttacga tcacttactt tacggtgtaa taaatttata aatactttca actcacaaat
       61 atgtgttttc cgggcatgat agatcctcaa atttggggtg agtcatgttt attagatttc
      121 tcatggaggt gtgtctattg attacagatg tgccatgtct taattgaaaa tcgtatgtcc
      181 cgatagttgt cgtgtgttgt gagtaaacta tgtcaaattc cgtgtaacta acttaggaga
      241 gaatatcatg acaatctcga acagaattat cttggtagtt gcgttactga ccatcgtctt
      301 cggaggggtc acattggctt tcgcttcagg caaagacaca atttatgctg ccgaagttgg
      361 tccggagatc gagggtacct ggttggcgac gattactatt ccggacgggc cgccgccatt
      421 tccgtcactc gtcacttatg ctcgtggtgg tgctctcacc gttaccgaca gtagcggatc
      481 accagcattg gggaatgttt accagggatc ctgggccaaa acgggtcccc acaaatacgt
      541 cttcactgtt atgggatttc tgtatgatga agagggtgtc ttcgcaggct acatcaggtt
      601 gcacgagatc gttaagatcg agcccggtgg caatgtttat aacggagaag catcgatcga
      661 gatccttgac cctgagcaga acgtggttat gactggtagc tctataagct acgccgaacg
      721 tatcaatcca tagcgactgg cgtcctcttc acccagaata gaagcgggtg ccgagcccct
      781 gggcattcct gacaacattt gtcgcgagta ggaatgcaga tttcgcgcac tccatccctc
      841 tccctgcctg gtcgaacgtc aggccagggt cgcctgtggg agagggacga tttttatcag
      901 ccgaaggtat gtccaagtga tggatgtgca aaagttgcag ggaatgtctt atatattgtc
      961 tgaacttcgg atcttgtctc ttaagcgcaa accttgtgtc tgcattgagc gggtggaaat
     1021 tctgttgaca tgccccttcc ctgacggtag aatgataaat cgtggatatt ctcgctggcc
     1081 tcaaccccca acagcagcag gcggtaatgg ctgggctggg tcccgtgctg gtgctggccg
     1141 ggcctggctc tggcaagacg cgcgtgctca cccaccgcat cggttacttg ctgggcgcga
     1201 tgggtgtgcg cccctaccag atcctggctg taaccttcac caacaaggcc gcccgagaga
     1261 tgcaaagccg cctgacccag ctcttggagg cgcgacccgg cgggtcgccc caatccgctg
     1321 atagccctca gatggggacc ttccatggcg cttgtgcccg catcctacgc agggaggcgg
     1381 agcacctgcc ttttcaatcc aactttgtca tctttgacgc cgatgatcag ttaaatgtcg
     1441 taaagactgc tattaaagac cttggtctgg atgacaagct ctaccgcccg ccaggcgtgc
     1501 atgcagccat atccaacgcc aagaatgagc tgctgctccc cgacgattac cccgtgcaaa
     1561 cttatcgtga tgaggtggtt gcacgcgtct accgtcgcta ccaggagctg ctgctggcca
     1621 gcaacgccct ggatttcgat gatctgctcc tgtggatgac gtacttgctg gataacgaac
     1681 cagcggtgcg cgataaatac gcccgccgct acgagcacgt gctggtggat gaattccagg
     1741 ataccaacat ggcgcaatac accttgctca aacacctggc ctccttccac cgcaacctgt
     1801 acgtggtagg tgatgtggac cagtctattt acggctggcg cggcgccgat tatcgcaatg
     1861 tgctgcgttt tgagaaggat taccctgatg ctcaggtggt cctgctggaa cagaactatc
     1921 gctcgacgca gatcatcctg gacgcagcca tgggggtgat cgaccggaac ccgcatcgca
     1981 cgccgcgccg cctgttcacc gaacgcggca gcgggcagaa gatcagcttg tacgaagctt
     2041 atgatgaccg cggtgaagct gccttcgtgg tggataccat cgccagcctg gtgggcagtc
     2101 gccgcgctca gccaggcgat ttcgctgtga tgtaccgtac caacgcacaa tcgcgtgtgc
     2161 tggaggaagc cttcctgcat gccgggctgc cctacaagct ggtgggagcg caacgctttt
     2221 acgggcgtcg tgaaatcaaa gatgtcattt catacttgcg cttggttcac aacccaaatg
     2281 acgagatcag cctgacgcgg gtgatcaata cgccggcgcg cggtgtgggc aataagaccg
     2341 tgctggcgct ccgcacgctg gctaagaaac agggactgtc ccctggcgtt ttgctgctgg
     2401 agctggggcg cgacccagat gcggcaggca ttgaggcgct ccccggacgg tcggctggtc
     2461 ctctggcgaa tttcgggcgc aacctggtcg gctggcgtga tttcgctaac attgccacac
     2521 ccttgagcct gatggaccgc attctgagcg atatcgacta ccaatcctac ctcgatgacg
     2581 gcagcgatga aggccgcgac cgttgggata atgtgatgga gttgcgccgc ctggctgctg
     2641 agtttcagga aaacgggctg gaagccttct tagagcaggt ggccctggtc tccgaccagg
     2701 atacgctgga agaacgcatc aatgttccca ccctgctgac cctgcatgcc gctaaaggat
     2761 tggagttccc ggtggttttt atcgtgggga tgaatgatgg caccttgccg cacagccgct
     2821 cgtttgatga tccggaggcc atgcaggagg agcgtcgcct tctatatgtg gggatcactc
     2881 gcgccaagga tcgcctgtac ctggtgtatg cccagaatcg cagtttgtat ggttacagcg
     2941 agccaatcga gccgtcacgc tacctggacg atatccccgt tgatctggtc gatgagacgc
     3001 aaccgacgcg ctcaggcaga ttacatcgcc cggctccacg aactgatcgc tggcaacccc
     3061 agaaagcctc cgtgcaatcg tcagaccagc cgcgttttca ccctggattg cgcgtgatgc
     3121 acaacgtctg gggagaaggc atggtgctca acagtcgcct tcaggacgat gatgagattg
     3181 tggatatttt ctttgaaagc acggggctca aacgtgttgc ggcttcctta gccaagctag
     3241 aagtcatgga gaaataggcg catccagaat tcggcaggac agattataat cttctcatgc
     3301 ccatactcga tccacattcc tttgagttta tcagccgcag cgcagagcaa acccgtcgcc
     3361 taggtatgcg cctgggtgct ctattgcggg cgggcgatgt aatctgcctg gtgggtgatc
     3421 tcggctcggg caagaccacc ctggtgcagg gcattgcctc tggatgggga tcgttagact
     3481 tggtaaccag cccaaccttt gtgctggtga acgtttattc cgggccggat gcagcgcgcc
     3541 tgtaccacct cgacgcatac cgcttgagta gcgctcgcga agccgaagat ctggatttgg
     3601 aacccatgct ggaaaccggg ctgttggtgg tggaatgggc tgaaagaatc caggaggcgt
     3661 taccgcgcga gcatctgtgg gtgaccctgc gctggattga taatgagcag cgcgatctgg
     3721 tcttcgctgc acggggcaaa cgttaccaat ccgtgctggc cagcgtgcgc aagcaaatat
     3781 ttgggattat gtgatgttat tggctttaga tacctcaacc cgagtggtag gtctggcgct
     3841 gtatgacggt gtccaagtgt tgggtgaaac cgtgtggttg agccgtgatt atcatactgt
     3901 cgagttggcg ccagcggtag ccaatatgct ggcgcgctca gatgtcaaaa tctcggatct
     3961 gagtgccctg gcggtagcca tagggccagg ctccttcact gggctgcgca ttggtttggc
     4021 gcttgccaag ggattggcgc tggcgcacag cttgcccctg gtgggcgtgc cttccctgga
     4081 tatactggct gccgcccagc cgatccaaga agaggtgatg gcggctgtct tgcgagccgg
     4141 acgggggcga ctggccgttg gctggtacca ggcatctgat ggtggctggc gcgccaaagg
     4201 gcaggttgag gcgcttacag cacaagagtt gaatgagcgc atccaggaac ctacgctggt
     4261 ttgcggcgag ctggaagagg atgaacagcg cctgctggcg cgcaagcgca agaacgttat
     4321 attagctcca gtggcccagt cagtgcggcg ccctgcctgg ctggcagagc tgggctggaa
     4381 gcgctggctg gctggcagag tggacgaccc caacacactc tcgcccatct atctgcatta
     4441 caacgagccg ataccgggat gatggctgtt cgatcgaaaa cttcgcccat cgatcctccc
     4501 cagcggtcaa atctcatcca tattcgcccg atgcggcagg acgacctaga tcaggtgatc
     4561 accatcgatc gggcatcgtt tagcttgccc tggccggaga gctcctaccg ctacgaattg
     4621 ctggagaatc cccattctct gctcatggtg gcggaggctg aggcgccgga tggggaaaag
     4681 caagttgtgg gggcagtggt ggtctggttg cttgaggatg aagcccacat tgcaacgctt
     4741 gcgatccatc cagagtaccg cggcagaggc atcagccagg agcttctggc gaccgtgtta
     4801 gccagatcga ttcggggagg cgcgcgaata gctaccctgg aagtgcgagc aaacaatcaa
     4861 attgctcacg ccctctatag gcgctttggc tttgaggttg tgggtcgccg cttgcgctat
     4921 tacctcgata acaatgagga tgcactgatt atgacggctg atggattgga tgaaagatac
     4981 cttgagtggc tagaatccca tgggtggcgc aagtgacctt gatgaaggca gctaagccgc
     5041 tgtatacttc agctcccggt agcaaatggt aagagaggca ctcaattaag gaggtgttgt
     5101 tttgaattct aaagagatct tagcgcaggt ttcacaggag gtgtctgcct gcacccaatg
     5161 tgttttgcac cattctcgca agaatgccgt accaggtgag ggtccggcga atgccaaaat
     5221 tatgttcatc ggcgaaggcc cgggtttcca tgaaaacgag cagggtcggc cgtttgtagg
     5281 tgcggcggga aaattcctgg aagatctgct gggaaagatc ggcatgcagc gcacggatgt
     5341 ctatattacc aatgtggtca agtgtcgccc tccagccaat cgtgacccta tggccgaaga
     5401 gctggaggct tgcaacgaat acctggagcg ccaaatccag gccatcaatc cgagggtcat
     5461 cgtcacatta ggacgctttt ccatggcgcg ttttctgtcc aatgcgaaga tcagcgatgt
     5521 gcacggccag gcgatgcgtg tgcgcggccg gttgattgtt ccgatgtacc atccagccgc
     5581 ggcgctgcat caaggctcgc tcagaccagt tatagagcgt gatttcgcat tgctccccga
     5641 gctgattgcc agcgccgcgg acgcgcccga ggtggaggag cctaagccac cagaggagaa
     5701 aacgcagcca agacaattga gtctttttta gcgtgaggaa ggatgagcgc aaatattaaa
     5761 cacaatctga tcgatcagat aaaggaacgt aaagcgcagg tagccatcct ggggctgggt
     5821 tacgtcggct tgccgctggc agccgtgatg gctgaagcag gttttcatgt ggttggtgta
     5881 gatcctgacc agcgcaaggt ggagatgatc agtcgcgggg agagccatgt caaggatgtg
     5941 cccagcgagc aagtggcgcg cctggtgaaa gcaggccaac tggttgccac cacagatttc
     6001 tctgtcttgc agaatgctca ggcggtcagc atctgtgtgc ctacgccgct gcgcaagacg
     6061 ggcgaccccg acctttcctt tatactggat gctacggctg agctggctag gtatattcac
     6121 cccggaatgg tggtcgtgct tgaatcaacc acctatcctg gtaccacgcg tgagatcctg
     6181 ctgccgcacc tgggtaacaa aaagggtttg gtggtgggcg agaatttctt cctggctttc
     6241 tcaccggagc gagttgaccc agggcgcaaa gactggacca cgctgaacac ccccaaagta
     6301 gtcggcggga tcactgctga ctgcggcgag gtagcgtcta tctggtactc gcaggcattg
     6361 cagtcggttg tgcctgtctc ctctacggag gtagctgaga tggccaagct gctggagaac
     6421 accttccgca tgattaacat tggcctggtc aatgagatgg ccctgatgtg caaccgcctg
     6481 ggtgtggacg tatgggaagt gatcgatgcg gctgcgacca agccttttgg attcatgaag
     6541 ttcacccctg gaccagggct gggagggcac tgcataccga ttgacccact ctatctttcg
     6601 tggaaactgc gcgccctcaa gtacactgcc cgctttattg agctggcatc tgagatcaac
     6661 acagccatgc cgcgtcacgt agtcgactta gtgcaggatg cactcaatgg gcagagtaag
     6721 gcattaaaag gaagccgagt actggtgctg ggggcagcct ataaacccga tatcgacgac
     6781 ctgcgcgaat cgcctgccct ggatgtaatt ggattactgg agcagaaggg tgctctggtt
     6841 tcttacaatg acccatatat
//
LOCUS       DBNGSX010000144         3371 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_149783, whole genome shotgun sequence.
ACCESSION   DBNGSX010000144 DBNGSX010000000
VERSION     DBNGSX010000144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_149783"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1390)
                     /gene="aspS"
                     /locus_tag="AC16CB_04790"
     CDS             complement(<1..1390)
                     /gene="aspS"
                     /locus_tag="AC16CB_04790"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR3341820.1"
                     /translation="MYKTHTCGELRSDHVGQNVTLAGWVHRRRDHGGVSFFDLRDRFG
                     LVQVVANSDISPESHSAGQGVRMEWVIQVEGIVRARPAGAENPNLPTGAIEVEVHNFK
                     VLNPAKTPPFLINKEEEADENSRLRYRYLDLRRERMRQNLILRHRMVKFIRDFLDERG
                     FLEIETPILFKTTPEGARDYLVPSRVHPGEFYALPQSPQQLKQLLMVAGVERYFQIAR
                     CFRDEDQRGDRQPEFTQLDLEMSFVEREDVLQLVEELFTKMVAEIVPHKRLLASPFPR
                     LTYAEAMSRFGKDNPDLRYGMELKDITDVAAGSGFKVFDAVVADGGHVRGLNASGCGD
                     YSRAQIEELTEYVKQYGAKGLAYLAYSSGGEQRSSFAKFLTPERLQMILERMEAKSGD
                     LLLFVADQPAIVYESLGRLRVFLAERFGLPDPDVLAFCWVIDFPFVNWNEDEQRWDPS
                     HHLFTSPMLEDIP"
     gene            complement(1446..2093)
                     /locus_tag="AC16CB_04795"
     CDS             complement(1446..2093)
                     /locus_tag="AC16CB_04795"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3341821.1"
                     /translation="MDASDSGINSIDVGGMLRQLRQERNMSMRALARASGLSTNALSM
                     IERSRTSPSVSTLYKLADALGVPITAFFRLEPPKQEIVFRKANQRSRLKIPRGNWEGL
                     GGESFIGGVEPLLLTLETGANSGQFAMLHTGHEFVMCLKGLLEYEVEKERFLLEPGDS
                     LLFAAQLQHRWRNVNKGVTTVIIVLSGFGRGERPSEYHLKTGTDEEVGLESRPKD"
     gene            complement(2319..2738)
                     /locus_tag="AC16CB_04800"
     CDS             complement(2319..2738)
                     /locus_tag="AC16CB_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR3341822.1"
                     /translation="MKAASRTLILSHTAIEKLKRHKKLQQLEIAAAEKWNYNNLIFSR
                     TDGEKYAPGSATEFHRRLMASLGLDHVRLHDLRHTAATLMLQEGIHPKAVQEIMGHAS
                     ISTTLGIYGHVTPGMREQAANVMDDLLAPIPVELKRS"
BASE COUNT          841 a          871 c          765 g          894 t
ORIGIN      
        1 aggggatatc ttctagcatt ggtgatgtga agaggtgatg gctgggatcc caacgctgct
       61 catcttcgtt ccagttgaca aacggaaaat ctatgaccca acaaaatgcc aacacatctg
      121 gatctggtag accaaatcgc tcagccagga aaactctcaa tcgtcccaag gactcatata
      181 cgattgcggg ttgatcagca acgaagagga gaagatcgcc cgacttggcc tccatccgct
      241 caaggatcat ttgcagccgt tctggcgtta aaaacttggc gaaacttgag cgctgttcgc
      301 cacccgagct gtacgctaga taggccaagc cttttgcacc gtactgcttg acatattcgg
      361 tcaattcttc aatctgagcc cggctgtagt caccacaccc gctggcattc agaccgcgta
      421 catgcccacc atctgcgaca acggcatcaa atactttgaa accgcttccc gccgccacat
      481 cagtgatatc ctttaattcc attccatacc gcaaatcagg attatctttt ccaaagcgtg
      541 acatcgcctc ggcatatgtc agccgtggga aaggcgacgc caaaagtcgt ttatggggga
      601 caatctccgc caccattttg gtgaacaatt cctccaccag ttggagcaca tcttcgcgct
      661 ctacaaacga catttcaaga tcaagctgcg tgaattctgg ctggcgatcg ccgcgctgat
      721 cttcatcgcg aaaacagcgc gcaatctgaa agtaccgctc gacacctgca accatcagca
      781 gttgcttgag ttgttgtggg gattgcggca gagcgtagaa ttcccctgga tggacgcggg
      841 aaggcactaa gtaatcacgc gcaccctccg gagttgtttt aaacaagata ggggtttcaa
      901 tttcgaggaa accgcgctca tctagaaagt cgcgtataaa tttgaccata cggtggcgca
      961 ggattaaatt ttggcgcatg cgctccctgc gtaaatccag gtacctgtag cgaagccggg
     1021 agttctcgtc ggcttcttcc tctttattaa ttaagaatgg cggtgttttc gctggattta
     1081 gtaccttaaa attgtgcacc tccacctcaa ttgccccagt aggcaaattg ggattttcag
     1141 ccccagcagg ccgtgccctt actatacctt caacttgaat cacccattcc atgcggaccc
     1201 cctgcccggc agaatgtgat tctggggaaa tatctgaatt ggctacgacc tgaaccaggc
     1261 caaatcggtc gcgcaaatcg aaaaacgata cgccaccgtg atcccggcga cggtgtaccc
     1321 atcctgcaag ggtcacgttt tgaccaacat gatctgaacg cagctcgccg caggtatgtg
     1381 tcttatacat aataaatcct ccccgatggg atacgttgaa tacacatatt caattcattc
     1441 tcctattaat cttttggcct agattccaac cctacttctt catcagtccc cgtttttagg
     1501 tgatattcac tggggcgttc ccccctccca aatccagaca aaacgatgat tactgtggta
     1561 actcccttgt tcacattccg ccagcgatgc tggagctggg cagcaaaaag caggctatcc
     1621 cccggttcga gtagaaatcg ttccttctcg acttcgtact caagaagtcc tttcaggcac
     1681 atgacaaatt catgacctgt atgcagcatg gcaaactgcc cactattcgc accggtctcc
     1741 aaggtcaaca atagcggttc taccccgccg ataaaagatt ctcctcctag gccttcccaa
     1801 ttcccccgcg gtattttcag cctggaacgc tggttggctt ttcgaaatac aatctcctgc
     1861 ttgggaggtt ctagccggaa aaaggcagtg atgggaacgc ccagtgcatc agcaagctta
     1921 taaagcgtac tgactgaggg cgaggtccgt gatcgctcaa tcatactcaa agcattcgta
     1981 gacaatccac tggcgcgtgc cagggctcgc attgacatat tccgctcttg tcgaagctgt
     2041 cgtaacatcc cacccacatc aatagaatta attccactat cagaggcatc catagcacaa
     2101 ctccggtcga gataatgcgc tattataatt gaatatttcc ttaccgtaca tatgggggta
     2161 gatatgtcct tactgcccac atatggcggt atttatctcc atattgtcct cacgttccac
     2221 tacgaaaaca tactgttgat gtaaaattga tgtaattccg acatcggaaa tgatgtcact
     2281 caatgatatc actccacatc ttgaaatgtg cagtgatttt atgatctttt cagttcaacg
     2341 ggaatcgggg ccagcaagtc atccatcaca tttgcagctt gttctctcat gcctggtgta
     2401 acatgaccat agataccaag tgttgtcgat atggatgcgt gacccatgat ttcttggact
     2461 gctttgggat gtatgccttc ttgcagcatc agcgtggcgg ctgtgtgacg cagatcgtgg
     2521 agcctgacat gatccaggcc aagacttgcc attaacctgc gatgaaattc ggttgcgcta
     2581 ccaggagcat atttttcgcc atctgttcta gagaatatga gattgttgta gttccacttt
     2641 tcggcggcag caatctcgag ctgctgcagt tttttgtgcc gttttagctt ttcaattgct
     2701 gtatggctaa gtatcagcgt tcttgatgcc gctttcatga gtttgctgtc ccaccatcaa
     2761 ctcctggcta tccaatggtt ggttgacgca agcacttcac catgccatca cctcgatata
     2821 gctataggtt gcgtgtttgc ggcagcacga caagatacgg ggagacgtgc tataccatta
     2881 ggatgtattt gcgctgggta tgactgccag attattcccc catatcatag tgaataatcc
     2941 tgattatttc gagcttttat gaggttgtat ttacgagtgc taatccccgc tagtttgtct
     3001 aaaatcaata tatctgtagt gaggtagatt gcggcattgt accggcttct gccgcatttc
     3061 cgacatcgga aatgccctct ttgcttaaat tgctggggca agacggggtc tgggacggcc
     3121 tggtagcttg ggaaaggctc cacagctatc ggtgtcgacc aatttcctaa attaaccaat
     3181 cacttacgtt acaaacacta atgtcttgct gtggaatatg cctgcacttt ccttcacaga
     3241 ataaaggata tacccccaaa atgaggcatg atatgtcatt ctttgacatt tccaatggtg
     3301 gagaagaact caaggctaac ggctggcgtt agcggcgact ggggagaaaa agcctcgaaa
     3361 acgcggcgcg t
//
LOCUS       DBNGSX010000145         2444 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1465662, whole genome shotgun sequence.
ACCESSION   DBNGSX010000145 DBNGSX010000000
VERSION     DBNGSX010000145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1465662"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..1842
                     /locus_tag="AC16CB_04805"
     CDS             322..1842
                     /locus_tag="AC16CB_04805"
                     /inference="COORDINATES: protein motif:HMM:NF014565.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004576 - oligosaccharyl transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STT3 domain-containing protein"
                     /protein_id="HIR3341823.1"
                     /translation="MSRANRLPLLLSLLAILAAYLVAERVFERMPHIEDEIAYLWQAQ
                     AIAGGHLTVPTPAHPKSFLVPFVVDYNGQRFGKYPLGWPAALAVGVFFGARQWVNPLL
                     AGLGVWLTYLLAKRLFGQTVGLLAALLTLTSPFFLMNSGSLLSHPFGLVLSTTFALAW
                     LDSWDHESMRPWLATIAAALALGLLAFTRPLTALGVSLPFLPHAAILWFREGWSTRRR
                     LLVFALIVLGVSSLIFVWQWAVTGDPLFNPYTLWWDYDKVGFGPGVGRLEVGHSLHQA
                     RINTEHSLWVGWHDYFGWGRFSWIFLPFGALALLRVRLAKALPVFLVFPSLVVIYLAY
                     WIGSSLFGPRYFYEGLYSLTIASAAGIAFLAGWPIHPGEPWMDRLGWARARPLLITAL
                     VALLLSANLLFYTPIRLGGMHGLYGISRDRLAPFQSAQAQGMIPALVIVHPDRWTEYG
                     SLLELTDPFLNTPFLVVISVGDNADAALAADYPDRAVYHYYPDRPWVFYKRPLNSP"
     gene            complement(1917..>2444)
                     /locus_tag="AC16CB_04810"
     CDS             complement(1917..>2444)
                     /locus_tag="AC16CB_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007504043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR3341824.1"
                     /translation="SGTPTKVDAVLAEADISSKVVEYTTSDGGVSLADAAVIVSGGRG
                     VSNNPALTPPAGLDEKQAEVWRAQQGFKLVSDLATVLGGAVGASRAAVDAGYIPYEHQ
                     VGQTGKVVSPDLYIACGISGAIQHQAGMRTSKMIVAINKDPEAPIFKMARFGVVGDLY
                     AILPPFTEALRKKLG"
BASE COUNT          420 a          706 c          684 g          634 t
ORIGIN      
        1 aacccggaga gaaaaaggcg gtctataagg gggtaatggc tggaacatac aaagcccccc
       61 atctcaaatt gctcagtcca aacaatagag tacttgaatc gctaaagcta attggcttag
      121 acctgtatat cgatattttt gatgacaaac aggaagcttt agcggctttt taactcccta
      181 aatactttca ctgagctccg ccctcaactt tccgaacttt caccccaact acctcccact
      241 ccgaggtggt aggtatcgcg accttcgatc tcattattcg cccgaattaa tggtacgata
      301 gattgaacgg ttttctcctt catgtctcgc gccaatcgcc tcccccttct acttagcctg
      361 ctggcaatcc tggctgctta cttagtcgcc gagcgcgtct ttgaacgaat gccccatatt
      421 gaagatgaga tcgcctatct ctggcaagcg caggcgatag caggcgggca cctgaccgtg
      481 cctacgcctg cccacccgaa gagcttcctg gtgcctttcg tcgtggatta caatgggcaa
      541 cgctttggta agtacccgtt gggttggcca gccgccctgg cggtgggtgt gttctttggc
      601 gccaggcaat gggttaaccc cttgctggct ggactgggtg tatggctgac atatctcctg
      661 gctaagcgcc tgtttggcca gactgtcggc ttgttagcgg cgttgctgac cctgacttcc
      721 ccattcttcc tgatgaactc cggctcgttg ctctctcatc cctttggatt ggttttgagc
      781 acaaccttcg ctttggcttg gctggactct tgggatcacg aatcaatgcg tccctggctg
      841 gcaacaatcg ccgcggctct ggccttaggc ttgctagctt ttacccgccc attgactgcg
      901 ctgggggtat cgctgccttt cttgcctcat gccgcgatcc tgtggtttcg cgaggggtgg
      961 tcgacacgtc gccgcctgtt agtcttcgct ttgatcgtct tgggtgtgtc gtccctgatt
     1021 tttgtctggc aatgggctgt gaccggtgat ccgttgttta atccttatac cctgtggtgg
     1081 gattatgata aggtcggttt tggaccgggg gtggggcgcc ttgaagtagg gcactctttg
     1141 caccaggcgc gcatcaacac cgagcacagc ctgtgggtgg gttggcacga ttactttggc
     1201 tgggggcgtt tttcttggat ttttctgccc tttggcgccc tggcactcct gcgcgtccgc
     1261 ttggcgaaag ccttgccggt gttcctggtc tttcccagcc tggtggtcat ttacctggcg
     1321 tattggatcg gctcttccct gtttggtccc cgttatttct atgaaggcct gtatagccta
     1381 accatagcct ccgcggctgg gatcgccttt ctggctggct ggcccattca ccctggtgag
     1441 ccctggatgg atcggctagg ctgggcgcgc gctcgcccgc tgctcatcac tgccctggtt
     1501 gctctgttgc tgagtgccaa cctgctcttc tacaccccca tccgcctggg gggtatgcac
     1561 ggattatatg gtatctcgcg tgaccgctta gcgccctttc aatctgccca ggcgcagggg
     1621 atgatccctg cgcttgtcat tgtccaccct gatcgctgga cggaatacgg tagcctgctg
     1681 gaattgacgg atcccttcct gaacaccccc tttctggtgg tcatcagtgt aggcgataac
     1741 gctgatgccg ccctggcggc ggattatcca gaccgggcgg tgtatcacta ttatcccgat
     1801 aggccatggg tgttttataa gcggccatta aatagtccat aagatcaacc tcccgcaggg
     1861 ttgaaccctg cgggaggttt ttttatctat tcattgagag gaagggacct gccctgctag
     1921 ccgagcttct tacggagagc ctcggtaaat ggcggcagga tggcgtacag gtcgcctacc
     1981 acgccaaaac gagccatctt aaagatcggg gcctcggggt ccttgttaat ggctacgatc
     2041 atcttgctgg tgcgcatgcc ggcctggtgc tggatcgccc cagagattcc gcaggcgatg
     2101 tacagatcgg gggagaccac cttgcccgtc tggccaacct ggtgctcgta aggaatatag
     2161 ccggcatcga cggcggcgcg gctggcgccc accgccccgc ctagcacggt agccaggtcg
     2221 gagaccagct tgaagccctg ttgggcgcgc cagacctcgg cctgtttttc gtccagcccg
     2281 gcgggtgggg tgagcgctgg attattggag acgccgcgcc cgccagagac gatgaccgcc
     2341 gcgtcggcca ggctcacacc gccatctgat gtggtatatt ccaccacttt gctggaaata
     2401 tcagcctctg ccaggactgc atcgaccttt gtcggcgtac ctga
//
LOCUS       DBNGSX010000146         2938 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_357716, whole genome shotgun sequence.
ACCESSION   DBNGSX010000146 DBNGSX010000000
VERSION     DBNGSX010000146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_357716"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2278)
                     /locus_tag="AC16CB_04815"
     CDS             complement(<1..2278)
                     /locus_tag="AC16CB_04815"
                     /inference="COORDINATES: protein motif:HMM:NF021504.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HIR3341825.1"
                     /translation="MRLQIGIPRAIQFYQHYPLWRTFFEELGADTVVSPPTNREIVTA
                     GAKVVADVTCLPVKVYAGHVTWLRDHGNVDFVFSPAIWNLEKDAFHCSKLKGLPDITK
                     ATVPNCPPLLVAEVDPYWRKLYENDAFYKVGRTFTRNSWKIRRAWALAKDADAKFQAL
                     IVREQITYPEAMARLYGSEWLTTERIVSKDAGLVIGLVGHPYCLYDDYLNHNLIRRLG
                     ELGVKVITSEMVPADAANRGIERTTRQTRWFFDAWMSGAAGYYLYEANVDGVIAALAF
                     GCGPDSTMVETINRRAQASGRSFLSLILDEHGSATGMVTRLEAFVDMLLRQKHSRLEK
                     DSTRVIEHRYPLKISPPALLSQIRRPVVGIPTMGTTAVPIKSLFKGIGAQVEMGPPVS
                     KQTVTLGAKSSPEFICTPYKQILGNMIEMLDAGADTLIYVDGLDLCRNSSYHQLLEDA
                     LSDLGYKFRLMTFAQIYEGGIFAMPKFLRQFAPELTWQDVVRELILGIAKIHAIDAIE
                     RRVQTIRPREVTQGTVDKLWVDALNRIEATKDMDALKRTKVDLLKAIDRIEIDPSRQP
                     VKIGLTGEIFAVLDPFYNQDIERELGRAGAEVHRTLMISNWLHGEMILGAFGFPHNKD
                     IERAAKPYLRANVSGEGWATIGETVICAEKGFEGMVELLPFTCEPEITALNILPRVSQ
                     DYNIPVISFIFDEQSGRAGIHTRVEAFVDLLYRRRELLESSTSFVSSSELPRQTFLGE
                     DVCNACPAIDNCQRNMLNA"
     gene            complement(2513..>2938)
                     /locus_tag="AC16CB_04820"
     CDS             complement(2513..>2938)
                     /locus_tag="AC16CB_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341826.1"
                     /translation="RWTNFGIGFGAGIGLHMLLDLLIWFNGVELLWPFGGWVNFWEAI
                     KPPEWFAKLLDPAEFAFLALFLAWLATTARKSQTNTDFLATLRRWTLVMVVLLIIFTP
                     LAYVMSKGFLTIFGVFYLISITAVFVITIRMRQTVEVLS"
BASE COUNT          708 a          752 c          743 g          735 t
ORIGIN      
        1 ttgcattgag catatttctt tgacaattgt cgattgctgg acatgcgttg catacatcct
       61 caccaaggaa ggtttgtctt ggtagttcgc tggatgatac gaaacttgtc gatgattcta
      121 gcaattctcg ccgccggtac agcaagtcga caaacgcttc cactcgtgtg tgtattcctg
      181 cacggcctga ttgttcatca aatatgaagg agatcactgg aatgttgtaa tcctgactta
      241 cgcgcggcaa gatgttcagc gcggtaattt ctggttcgca agtaaatggc aggagttcga
      301 ccatgccttc aaatcctttt tcggcacaga tgaccgtttc gccaatagtt gcccagcctt
      361 ctcctgagac attcgcgcgc agatatggtt tagcagcgcg ctcgatgtct ttgttatgtg
      421 gaaaaccaaa cgcacccaat atcatctcac cgtgcagcca gttgctgatc atcagcgtgc
      481 ggtgtacttc cgcccctgct cgtcccaatt cacgttcaat atcctggtta taaaatggat
      541 ccagcacagc aaatatctct ccagtgaggc cgatctttac cggctgtctt gatggatcga
      601 tctcgattcg atcgattgct tttagaagat ccactttggt gcgtttgagt gcatccatat
      661 cttttgtggc ttcaattcga ttaagcgcat ctacccacaa tttgtcaacc gttccctgag
      721 tcacttcccg cgggcgaata gtttgcacgc gccgttcgat tgcatcaatc gcgtgaattt
      781 ttgcaattcc gagaattagt tcccgcacaa catcctgcca ggtaagttcg ggagcgaatt
      841 ggcgcaggaa ttttggcatc gcaaatatcc cgccttcata aatctgagca aaggtcatta
      901 acctgaattt atagcccagg tcactgagtg catcttccag taattgatgg tagctgctgt
      961 ttcggcatag atcaagccca tcgacataga tgagcgtatc tgctccggca tccagcattt
     1021 cgatcatgtt gccgaggatt tgcttatagg gtgtgcagat aaactcgggg gaagatttag
     1081 cgcctagtgt caccgtctgc ttgctgacag gcggccccat ctccacttgc gcgccgatcc
     1141 ccttgaacag ggattttata ggtacggctg tggtgcccat cgtagggatg cccaccactg
     1201 gcctgcggat ttgggaaagc aatgctggtg gggagatttt aagtgggtat ctgtgctcaa
     1261 taacgcgggt tgagtctttc tctaatctag aatgtttttg gcggagcagc atatcgacaa
     1321 aggcctccaa gcgggttacc atgccggttg cactgccatg ttcatcaagt atcaggctca
     1381 agaaagaacg gccagaagcc tgcgcgcggc ggttgatggt ctcgaccatg gtcgaatcgg
     1441 gcccgcagcc aaaagccaga gcagcaatga cgccatccac attggcttca tacaggtaat
     1501 agcctgcagc tccagacatc catgcatcga aaaaccagcg agtctgccgg gttgttcgct
     1561 caataccgcg gttggcggca tctgccggga ccatctcgct ggtaatgact ttaactccca
     1621 attcccccaa tcgtctaatc aggttgtggt taagataatc atcgtagagg cagtaggggt
     1681 gtcctaccag gccaatgacc agcccggcat ccttggatac aattcgctcg gtggtcagcc
     1741 attcggagcc atacaagcga gccatcgcct ctggataagt gatttgttcc ctaacgatga
     1801 gtgcctgaaa ttttgcgtcg gcatcctttg cgagcgccca ggctcgacgg atcttccatg
     1861 aattccgcgt gaacgtccgg ccaaccttgt agaaggcatc attttcgtaa agttttcgcc
     1921 agtaagggtc aacttcggcg accagcaatg gcgggcagtt tggcacggtt gcttttgtga
     1981 tatccgggag gcctttgagc ttcgagcagt gaaaagcatc tttttccagg ttccaaatag
     2041 cgggtgaaaa aacaaagtcc acatttccgt gatcacgcag ccaagtgaca tgccccgcgt
     2101 aaactttcac tggcaggcag gtgacatcgg cgaccacttt ggctccggcg gttacaatct
     2161 ctcgattggt tggcggggag acgactgtgt ctgcccccaa ttcttcaaag aatgtgcgcc
     2221 agagagggta gtgttgataa aactgtatcg cgcgcggaat accaatttgt agtctcattg
     2281 actccctcgc tcatcattga cagatcctag tcgtaattaa cattagactt gatggcacag
     2341 gctccaatag gtagattttt agtctgggca cgtttaaata tctatcgggc gagatcaatg
     2401 cccacaactt cagcaccttg gctggcagat gaatttcatg aagttgtata aggaggcaga
     2461 gtgtttgtaa tgacggagga tgtgcttaag atctgacctc cttaggtaaa tattaactca
     2521 acacctccac ggtttggcgc atgcggatcg tgatcacaaa caccgccgtg atcgagatga
     2581 ggtagaaaac gccaaaaatg gtcaagaacc ccttgctcat cacgtacgcc aggggagtga
     2641 agattatcaa caagacaacc atgacgagcg tccatctgcg cagcgtggcc agaaaatcgg
     2701 tgttcgtctg ggatttgcga gcagttgtcg ccagccaggc cagaaacagg gccaagaagg
     2761 caaactcggc cggatccagc agtttggcga accactcagg cggcttgatg gcttcccaaa
     2821 aattcaccca accgccaaac ggccacagca gttccacgcc attgaaccag atcagcaggt
     2881 ccaggagcat gtgcagaccg atgccggccc caaagccgat gccgaagttc gtccagcg
//
LOCUS       DBNGSX010000147         1576 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1050637, whole genome shotgun sequence.
ACCESSION   DBNGSX010000147 DBNGSX010000000
VERSION     DBNGSX010000147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1050637"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="AC16CB_04825"
     CDS             complement(<1..359)
                     /locus_tag="AC16CB_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HIR3341827.1"
                     /translation="MKELIQTLVEIPGPSGYESKIREAIRLQIEPLVDETRIDALGNL
                     IARKGQSQGDGQKIMLAAHMDEIGLIVTHVDENGFARFTTLGGVRAQNLPGGRVRFMN
                     GASGVIGVQYHGEPKASA"
     gene            complement(356..1429)
                     /locus_tag="AC16CB_04830"
     CDS             complement(356..1429)
                     /locus_tag="AC16CB_04830"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017181.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIR3341828.1"
                     /translation="MMTPNSDKKSPKIGAAQIRLLERLTQACAVSGDEGEVRAIVLEH
                     IRPVADDVRVDALGNVLATRRGQVEPRLRVLLTAHMDEVGFILTHDEDNGFFRFERVG
                     GIDVRYLPGKSVWVGKDHLPGVIGAKPIHLATKEELRQTISQDTLRIDVGPGNGSKVK
                     PGDRATFATSFTRLGASLRAKALDDRLGVATLIELVKHAPANIDLLAAFTVQEEVGLR
                     GAGVAAYALNPDLAIILDSTPANDLPAWDESENTKYNTRLGAGPAIYMADRGTISDPR
                     LVRHLVETAEAAGIPYQIRQPGGGGTDAGAVHKQRAGIPSVSVSVPARYNHTPAAIAR
                     LSDWKNTLALLHAALKQLTAEIL"
BASE COUNT          340 a          470 c          426 g          340 t
ORIGIN      
        1 gccgaggctt tgggctcgcc gtgatattgc acgccgatca cgcccgaggc gccattcatg
       61 aaacgcaccc tacccccagg aagattctgg gctcgcacgc ccccgagcgt ggtaaagcga
      121 gcaaaaccat tctcatccac atgggtcaca atcaaaccta tctcatccat gtgggcggcc
      181 agcatgattt tctggccatc cccctgggat tgccctttgc gggcgatcag gttacccagg
      241 gcatctatac gcgtctcatc caccagcggc tcaatctgaa ggcggatagc ctcgcgaatt
      301 ttggattcat agcctgaagg gccaggtatt tccaccaagg tttgtatcag ttctttcata
      361 ggatctccgc tgtaagctgt ttgagcgcgg catgcaatag cgccagagta ttcttccaat
      421 ccgataagcg ggcaatggcc gccggggtat ggttatagcg cgccggtaca gagacggaca
      481 ccgagggtat tccggcacgc tgcttgtgca ccgccccggc atctgtgcca cccccgccgg
      541 gctggcgaat ttgatacggg ataccagcag cctcagcggt ctcaaccaag tggcgcacca
      601 agcgcggatc ggaaatcgtg ccccggtcag ccatgtagat cgccgggccg gcgccgagtc
      661 gcgtgttgta ctttgtgttc tccgattcgt cccaggcagg caggtcgtta gcaggggtac
      721 tatcgagaat aatagccaaa tctgggttca gagcatatgc agcaacacct gcacctcgca
      781 agcctacttc ctcctggacg gtgaaagccg ccaggagatc aatgttcgcc ggagcatgct
      841 tgaccagttc gatcagggta gccacaccca gtcgatcgtc cagcgcttta gcgcgcaagc
      901 tggcacctag tcgagtgaaa gacgtagcaa aagtagccct gtcgcctggc ttgaccttcg
      961 acccattacc gggtccgaca tcgatgcgca gcgtatcctg tgatatcgtc tgccttagct
     1021 cctccttggt tgcaaggtgg atcggtttgg caccgattac gccgggcaaa tggtcttttc
     1081 ctacccaaac tgacttgccg ggcaagtagc gcacatcgat accgcccacc ctctcgaagc
     1141 ggaagaagcc gttgtcttca tcatgcgtga ggataaaacc cacttcgtcc atgtgagcag
     1201 ttaataagac ccgcaggcga ggctctacct ggccgcgccg cgtagccagc acgttgccca
     1261 gggcatctac tcgcacgtca tccgcgacag gacgaatgtg ttccagaaca atggcgcgca
     1321 cctcgccctc gtcgccggag acggcgcagg cttgtgtcag gcgttccagc aaacggattt
     1381 gggctgcgcc gatctttggg gattttttat cgctgttagg tgtcatcatt ttacaattca
     1441 catatcatgg gccatgtgta ccaaactgag tgttgcataa tcattggcaa ctggcacagg
     1501 gtcaatccca ggtcagcttg tccataaact cagcatctaa gctaacggca aactcggcca
     1561 acaagcgtcc agcgcg
//
LOCUS       DBNGSX010000148         9385 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1603956, whole genome shotgun sequence.
ACCESSION   DBNGSX010000148 DBNGSX010000000
VERSION     DBNGSX010000148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9385)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9385)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9385
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1603956"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="AC16CB_04835"
     CDS             complement(<1..467)
                     /locus_tag="AC16CB_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013486.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HIR3341829.1"
                     /translation="MSEFTNKISLLRDLVARHDLDAVLLRRASNFAWITCGASGYINI
                     ASSEAEAYLLVTADRQFLITNNIEAPRLDKEEALTAQGWEFRVSPWYETRDAIAELTH
                     GLKTGADVPLPGAVNLMEKITHLRAKLTPEEGERFRTLGRLCAQAIETAARSVR"
     gene            complement(542..2047)
                     /gene="araA"
                     /locus_tag="AC16CB_04840"
     CDS             complement(542..2047)
                     /gene="araA"
                     /locus_tag="AC16CB_04840"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HIR3341830.1"
                     /translation="MMEFKNLEVWFITGSQHLYGEATLKKVADHSQEIAREMTQSGKL
                     PIKVVYKPVVTTPDAVTAICREANNAPNCVGLITWMHTFSPAKMWIAGLNALDKPFVH
                     LHTQYNRDLPWADIDMDFMNLNQSAHGDREFGFIGSRMRLRRKIIVGFWQDEQVLAKL
                     GIWMRAASAWHDMQGMKIARFGDNMREVAVTEGDKVEAQIRLGYAVNGYGLGELVNEV
                     KAVSDAEVDRLVAEYDERYLVAEALTPKGSQREALRQAARIEAGLRTFLEKGRFKAFT
                     TTFEDLHGLTQLPGLAVQRLMAEGYGFGAEGDWKTAALVRAMKVMGAGLEGGTSFMED
                     YTYDLDETTPQVLGAHMLEVCESVAEGKPSLEIHPLSIGGKSDPPRLVFDIRNGPAVN
                     ATLLDMGNRFRMVVNEVDVVPPKRPLSKLPVARAVWVPRPDLKTAAEAWIMAGGAHHT
                     GFSQAISAEYLEDFSEMAGLEFLSIDNSTQITEWKKELRWNEMYYLLAKAL"
     gene            complement(2077..3117)
                     /locus_tag="AC16CB_04845"
     CDS             complement(2077..3117)
                     /locus_tag="AC16CB_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR3341831.1"
                     /translation="MSIHNKRPTIIQVARAAGVSTQTVSRVINDRPDVAPETRKRVKQ
                     VIDQLGYQPSALARSLSQRRSYTLGVVTAGLKYTGPSRTLNGITTKAEELGYALLLKE
                     LPSFTTNDIQSLIQFLLARHIDGLLWAVPEIGNNRKWIEMGVDKIPVPIVFITMQSRP
                     GYSIVSVDNHRGGILATQHLLDLGRQHIGHVSGPLEWWEARQRKQGWQEALHAAGRKV
                     TETHCAEGNWSASSGETAFLQLLQSYPELDAVFVANDQMALAALQIAHRRNLHIPKDL
                     AVIGFDNIAESAYFYPSLTTINQNQHELGCQAVQELVSLIEAAHSGNQAIEPKSFLLA
                     PELILRESSVVR"
     gene            complement(3758..4822)
                     /locus_tag="AC16CB_04850"
     CDS             complement(3758..4822)
                     /locus_tag="AC16CB_04850"
                     /inference="COORDINATES: protein motif:HMM:NF025839.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HIR3341832.1"
                     /translation="MYTYGRKTSCRISLDYTYRGMRVAFLENDLIRVGVLLDKGADIF
                     EFTFKPVDLDFMWQSPIEMRKPFVATSALPEGAFHDYFYGGWQEVLPSAGWASEPYQG
                     TFQGLHGEVSLLPFEANITEDTPEVVSLRTWVRMYRTPLKLERTLTLHRSKSALFIHE
                     RLVNESPGEFAIMWGHHPAFGEPFLDEHCVVRAPAKKVEVLAFHPNGLWEPGGDYEFP
                     MVPNRRTGQLQDITHVLGRSARSVDVVFFKELSEGCYTLTNEKMDVGFGMAWDVSLFK
                     YLWMWQVYAGHDDYPWYGRTYNCALEPFTSYPPAGIQNAMRNGSAYILMPDEVIETDL
                     VAVAYQSITPSRVSLDGTVS"
     gene            complement(4934..5923)
                     /locus_tag="AC16CB_04855"
     CDS             complement(4934..5923)
                     /locus_tag="AC16CB_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015500608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3341833.1"
                     /translation="MDQIAKFFRSTVAGPLVAFVVVAILASLATPRFLLPQNLSNVSL
                     QVATVAVVSIGATLVILTGGIDLSPGSTVALVTCTLAILVKNMGLPLPLGIFLVLLLG
                     AVCGFINGFFSTFMKIPSFIVTLATMSIYRGLAFLITNGTPIFSVSPELEPIFYGKFL
                     GIPLPFYYVITLYLLGAFFLRSTVQGRAIYAVGGNESAARLTGIRVNRIRLLAFILAG
                     LSASIGGVLLTAWLNSGSPNYGAELGLQSIAAAVIGGASLTGGYGSMIATLIGALTVA
                     VVQNGLNLLAVPASWQEITLGMVIVLAVGLDMWRADIGRGFRRLFRISSGKPG"
     gene            complement(5907..8027)
                     /locus_tag="AC16CB_04860"
     CDS             complement(5907..8027)
                     /locus_tag="AC16CB_04860"
                     /inference="COORDINATES: protein motif:HMM:NF013757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3341834.1"
                     /translation="MSVFTEEDTLTSMAPTGEPLWELRNISKAFPGVQALDDVSFEVY
                     PGEVHALLGENGSGKSTLAKCLAGVHQAESGQMLFKGREVIFRNPMEARQMGIATIYQ
                     EFSLVPTLTVAENIFLGRYPRNAAGTIDWNTARKATSEVLESLSLTIDPDAIVKGLSV
                     AEQQLVEIAKAISLKSNLLIMDEPTAALGLVETQHLMELIHRLTAQNKAIIYISHRLD
                     EVFQIADRVTILKDGRRVKTARLADFQMGEVVRLMIGFDIQQHYPKAHNTQAIPCLEV
                     EDLSTENGVHNVNFTVNVGEVFGLGGMLGSGRTEIARAIFGMDKLAYGRLRLNGREVR
                     FRSPSEAVAAGIGLIPENRKTDGCFFNFEAPKNITISRLREVLSGPFLSLSKEYQVGK
                     SYISKMNIHPTALEKSVKFLSGGNQQKVIVARWLFSQARLLIMDEPTQGIDIGAKLEV
                     YNVINELTAAGISILLISSDFPELLAMSDRVAIVRDGHILHITEAHRMSEYQLIGMAS
                     GIGLSQEIEMWMDLRKIAHPYLQSLRERTGFIVHLGVMERDEMTLLYLDKVGYGSSDM
                     ISRSGASGPLSCTGLGKVLLAFEPPEKVRAWVEENGLPQYTENTITDPQAFISEMKTI
                     SQQGFGLDLEEHEPGVRCIAAPIYSKSGEVVAAISISGPTEKMPSEPGSSPIREEVQS
                     IAEAISREFATNHSAGENPHGSNR"
     gene            complement(8263..>9385)
                     /locus_tag="AC16CB_04865"
     CDS             complement(8263..>9385)
                     /locus_tag="AC16CB_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007196187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR3341835.1"
                     /translation="YPGGWTETPTVNTDKDNAGSFLCKPENLYPKPSKEFACEAAPME
                     EGYTPPEGAMPPVPLAEGSQSTTPTAYKAGDPIKIAYMPPATEFNYYMAIGEGIKALA
                     AEAGVETFMLAPQSGSDIATQMKMLQDVTTQDVDAIILSTHDENAAAPLVKQAVDMGI
                     AVVIVNSDIASFPSPVNAVVGYSQRGGTYDLGKYAAEMVGGTAKVGVLEGLPGYHSTE
                     RIGGFLDAFKEYPGMEIIASLPTAWNVETGNTAMMDLLQAHPEVNLVVCANDYIAIGA
                     ATAAEALGRSDVLIFGNDGDTAGLEDIYNGKWTATVNTTPFVMGKVALQVALDVLNGT
                     YPGGWTETPTVNTDKDNAGSFLCKPENLYPKPSKEFACP"
BASE COUNT         1978 a         2572 c         2602 g         2233 t
ORIGIN      
        1 cgcacagaac gggcagcagt ttcgatcgcc tgggcgcaga gccgtccaag tgtgcggaag
       61 cgttccccct cctcaggcgt cagtttcgct ctcaggtggg ttattttctc cataagattg
      121 accgcaccgg ggaggggaac gtctgcgcca gtttttaagc cgtgggttaa ttcagcgatg
      181 gcatccctcg tctcatacca ggggcttacc cgaaactccc atccttgcgc ggtgagggcc
      241 tcttctttgt ccagacgggg cgcctcgata ttatttgtga tgagaaactg acggtcagct
      301 gtgactagta aatatgcttc ggcttcgctg gaagcgatat ttatgtatcc tgatgcccca
      361 caggtgatcc aggcaaaatt gctggcgcgc cgcagcagga ccgcgtccag gtcgtgcctg
      421 gcaaccagat ctcggagcag gctgattttg ttggtgaact cactcatcga cctgttctct
      481 tgtgactaac aagcttgctc tatttctgcg ttcggcgaat tgcgattcct ctcggcttgc
      541 gtcatagggc tttcgccagg aggtaataca tttcgttcca acgcagctct tttttccatt
      601 cggttatctg tgtgctatta tcaatcgata gaaactccag acctgccatc tctgaaaaat
      661 cctctagata ttccgcgcta atggcctggc tgaatccggt gtggtgggcg ccgcctgcca
      721 ttatccaggc ttcggctgca gtcttgagat ccggccgggg cacccagacc gctcgtgcga
      781 ccggcagttt tgacaagggt cgctttggcg ggaccacatc cacttcattc acaaccatgc
      841 gaaagcggtt gcccatatcg agcagggtgg cattgaccgc tggaccattg cggatgtcaa
      901 aaaccagcct gggcggatcg gatttcccgc caatggagag gggatgaatc tctaggctgg
      961 gttttccctc ggcaaccgat tcgcaaacct cgagcatgtg tgcgccgagc acctggggtg
     1021 tagtctcatc caggtcgtac gtgtagtcct ccatgaatga cgtcccgccc tccagtccgg
     1081 ctcccatgac cttcatggcg cgtaccaagg cggcggtttt ccagtcccct tctgcgccga
     1141 agccgtagcc ctctgccatc aggcgctgga ccgccaaacc cggtagttgt gttaatccgt
     1201 gcaaatcttc gaaggttgtg gtgaaggcct tgaatcggcc tttttccagg aacgtcctta
     1261 accctgcttc gatgcgggct gcctggcgca aggcttcacg ttggctaccc ttcggcgtca
     1321 aggcctcggc aacgagatag cgttcgtcgt attcagctac cagcctatcg acctcggcat
     1381 ccgatacagc cttcacctca ttcaccagtt cacccagtcc gtatccgttg acagcatatc
     1441 ccaggcggat ttgagcttct accttgtcgc cctcggtgac tgccacttcg cgcatgttat
     1501 cgccaaagcg ggcgatcttc atcccttgca tatcgtgcca ggcagaggca gcccgcatcc
     1561 agatgccaag ttttgccagg acttgctcgt cctgccagaa accgacgatg atcttccgcc
     1621 tcaatcgcat gcggctgccg ataaatccaa attcccggtc gccgtgtgcg gactggttga
     1681 gattcataaa atccatgtcg atgtcagccc acggcaggtc gcggttgtac tgtgtatgga
     1741 ggtgaacgaa gggcttgtcg agcgcattca atccagcgat ccacattttc gctggagaga
     1801 aggtgtgcat ccaggtgatc agcccgacac aattgggggc gttattcgcc tcccggcaga
     1861 ttgcggtgac cgcatcgggg gttgtcacga ctggcttgta gacaaccttt atcggcagct
     1921 tgcccgactg ggtcatctct cgcgctattt cctgcgaatg atctgccact ttcttgagcg
     1981 tggcttctcc atatagatgc tggcttccgg taatgaacca aacttccaga tttttgaatt
     2041 ccatcacaat gcctccctaa gataagcatt aggcggttat cgaacgactg agctctcccg
     2101 caaaatcagc tcaggtgcaa gaaggaatga tttaggttct atggcttgat tgcccgagtg
     2161 ggcagcttcg atcaggctga ccagctcctg cactgcctgg cagcctaatt cgtgctggtt
     2221 ttgattaatg gtggtgagag aaggatagaa gtaagcggat tcggcgatgt tatcgaagcc
     2281 aatcacggcc agatcctttg ggatgtgcag gtttcgacgg tgggctattt gcaatgcagc
     2341 caaggccatc tggtcgttgg cgacgaagac cgcgtccaat tctggatagg attgcagcaa
     2401 ttgcaggaag gcggtttctc cgctggatgc ggaccagttt ccctccgcac aatgtgtctc
     2461 agtcactttc cgacctgcgg catgcaacgc ctcctgccag ccctgtttgc gctggcgcgc
     2521 ttcccaccat tccaggggtc cggaaacgtg gccaatgtgc tggcgtccca gatcaagcaa
     2581 gtgttgcgtt gccaatatgc cgcctcgatg gttatccacg ctgacaattg agtaccctgg
     2641 gcgcgactgc atggtgatga atacgatcgg caccgggatt ttgtcgacgc ccatttctat
     2701 ccatttgcgg ttgttgccaa tttcgggcac tgcccatagt agaccatcaa tgtgtcgggc
     2761 gaggagaaat tgaatcaacg attggatgtc attggtggta aagctgggaa gctccttgag
     2821 gagcaaggcg taccccagtt cttcggcttt ggttgtgatg ccgttcagag tgcgcgaggg
     2881 gccagtgtat ttcaatccag ccgtgacaac gcccagcgtg tagctgcgtc gctgggacag
     2941 gctgcgagcc agagcgctgg gctgatagcc aagttgatca atgacctgct tcacacgctt
     3001 gcgagtctcc ggagctacat cggggcggtc attgattacc ctggagacag tctgcgtgga
     3061 gactccagca gcccttgcta cttgtatgat ggtggggcgc ttattatgaa tactcatcac
     3121 agggaaaacc ctttagaggg ctttctgtta tcgttatcgg gaacgataac agaatagcaa
     3181 atctgggcat gtttgtcaag aggagaccga gttccgccag ctggactgtc cggaatcgcc
     3241 agcatctcaa agtaggcacc aactagtgcg gtgtggtttg tttgaggtag gggggagttg
     3301 gaaggggctt ataaacagtt cattgtgata tggcgtgtat ttggtgcagg tccatcaaag
     3361 agaaacttcc gagataatct tctcggaagt tgaattttgc tcgcacgaaa atattgggcg
     3421 gttaggccgg cgcttggctc attaaaggcc taaatttata ccggtatgtt tgatataaca
     3481 aaaattctat gatttagcca attgttttgt tgtaagatta ctcactctgg gttgaagggg
     3541 gcttccatgc ttatcggctc ggctgtcgtc tggcaagccc gaacttcggc tgaatgccag
     3601 tgcacctgcg cctaatctcc aaattcctcc accgctcatt ttacagactc cttcatcagc
     3661 atcggacaaa tcccctccga aggggttgga ttaatccaaa aagaggtcat atggggtgtt
     3721 taggcttcat atgacctctt tttgggtctt tcccttacta gctgactgtg ccgtccagac
     3781 tcacccggct gggggttata gactggtaag ccaccgccac taggtctgtc tcgatgacct
     3841 catctggcat caagatgtac gccgagccat tgcgcatggc attctggatg ccggctggtg
     3901 gatagctggt gaacggttcc aaggcgcagt tataggtgcg cccgtaccag gggtaatcgt
     3961 catgccctgc atacacctgc cacatccaca ggtatttgaa caggctgaca tcccatgcca
     4021 tgccgaagcc cacatccatt ttttcgttgg tcagggtgta gcaaccttcg ctcagctctt
     4081 taaagaaaac cacatccacc gagcgcgctg accgccccag gacgtgggtg atatcctgta
     4141 actggcccgt gcgccggttg ggcaccatgg ggaattcata atctccgccc ggctcccaca
     4201 ggccattcgg gtggaaggcc agtacttcca cctttttcgc tggcgccctg accacacaat
     4261 gctcgtccag gaaaggctca ccaaaagctg ggtggtggcc ccacatgatt gcgaattcac
     4321 ccggagattc attgaccagc cgttcatgaa tgaacagcgc agatttgctg cgatgaaggg
     4381 tcagtgtacg ctccagcttc aagggtgtgc ggtacatgcg tacccaggtg cgtagtgaga
     4441 ccacttcagg cgtgtcctcc gtgatgttgg cttcaaaggg tagcagtgaa acctcaccgt
     4501 gcaagccttg gaaggtaccc tgatagggct cagaagccca gccagcagag ggcagcacct
     4561 cctgccagcc accatagaaa taatcgtgaa aagcgccttc gggaagggcg ctggtagcga
     4621 cgaaaggctt gcgcatctcg atgggcgatt gccacataaa gtcgagatct acgggcttga
     4681 aggtgaactc gaagatgtct gcccctttgt cgagcagtac acccaccctg atgaggtcgt
     4741 tttccaggaa ggccacgcgc atccctcggt aagtgtaatc aaggctaata cggcagctgg
     4801 tctttcttcc atatgtgtac atcttgatgc tctcctatct caggtaattc ttcgccacaa
     4861 aggtcacaaa gtggcaccaa ggaagaaaat gtttgatgta ttttgtgatc cttcgtgtct
     4921 aatcgatcca gtcctaacca ggttttccgc tggagatcct aaacaggcgc cggaagccac
     4981 gcccgatgtc cgcccgccac atgtccagcc ctactgccag cacgatgacc attcccaggg
     5041 tgatctcttg ccaagaagct ggcacagcta gcagattgag cccgttttgc accactgcca
     5101 cagttaacgc cccaattagg gtggcgatca tgctgccata gccgccggtc aggctggccc
     5161 cgcctatcac tgccgcggcg atagactgta agccaagctc tgccccgtag ttgggggaac
     5221 ccgagttcag ccaggcggtc aataaaaccc cacctatgga ggcgctcagc cctgccagga
     5281 taaaggccaa cagccggatg cggttaacgc ggatgcctgt caggcgcgct gccgattcgt
     5341 tgccgcccac ggcataaatc gctcgccctt gaaccgtgct gcgcaggaag aacgctccta
     5401 gcaagtatag ggttatgacg taataaaatg gcaggggaat acccaaaaat ttaccataaa
     5461 aaatgggctc gagttcaggc gagacagaga aaatcggggt gccattggtg atcaagaaag
     5521 ccaatccccg gtaaatgctc atggttgcaa gggtaacgat gaaagaaggg atcttcatga
     5581 acgtgctgaa aaacccgtta ataaacccac agacagctcc cagtaataga acgaggaaaa
     5641 tacctaacgg caggggcaaa cccatattct tgaccaggat cgcaagtgta caggtcacca
     5701 gggcgaccgt ggacccgggg gataaatcga tcccgcctgt gaggatcacc agcgttgcgc
     5761 caatagagac aaccgcaacc gttgctacct gcaaggatac attgctcagg ttttgtggca
     5821 ataagaagcg gggtgtggcc aggctggcca ggatcgccac cacaacaaag gccaccagag
     5881 ggccggcaac cgtggagcgg aagaatttag cgatttgatc catgtgggtt ctctcctgct
     5941 gaatgattgg tggcaaattc cctggatatg gcttctgcaa tcgattgaac ctcctccctt
     6001 attgggctgc tgcctggttc ggagggcatc ttctcggtag gtcctgagat gctgatcgca
     6061 gcgaccactt ccccgctttt gctgtatatt ggcgcagcga tgcagcgaac accaggctcg
     6121 tgctcctcca gatcgagccc aaatccctgc tggctaatgg tcttcatttc ggaaatgaat
     6181 gcctgggggt cagtgatggt gttctcagta tactgtggta ggccattctc ttcgacccat
     6241 gcacgtactt tttctggtgg ttcgaaagcc agcaatacct tccctaaccc tgtgcagctt
     6301 agtggtcctg aggcccctga gcgtgagatc atgtctgatg agccataccc aactttatcc
     6361 aagtagagca acgtcatctc gtcacgttcc atcacgccaa gatgtacaat gaagcctgtt
     6421 cgctcgcgta gcgattgcag gtaggggtgg gcaatcttgc gcaggtccat ccacatctcg
     6481 atctcctggc tcaagcctat tccggatgcc atgccgatca actggtattc agacatacgg
     6541 tgcgcttctg tgatgtgcaa gatatgcccg tcgcgcacga tggctacccg gtcgctcatc
     6601 gccagcagtt cggggaaatc ggagctgata agcaggatgc tgatacccgc cgctgtcagc
     6661 tcgttgatga cgttatacac ctccagtttg gcgccgatat cgatcccttg ggtgggttca
     6721 tccatgatca gcaggcgtgc ctgcgagaac agccagcgcg ccacgatcac cttttgctgg
     6781 ttgcctcctg agaggaattt aacggatttc tccagggcag ttgggtggat gttcatcttg
     6841 ctgatgtagc tcttccccac ctggtactct ttggaaaggc tgaggaaggg gccgctcagc
     6901 acttccctca accgggagat cgtgatgttc ttcggggctt caaaattgaa gaaacagcca
     6961 tccgtcttgc ggttctcagg gatcaacccg atcccggctg ctacagcctc gctcggtgaa
     7021 cggaagcgca cttctcgccc attcaaacgg agtcttccgt acgccagctt atccataccg
     7081 aagatagcac gtgctatctc cgtgcgaccg gagccgagca tcccacccaa tccaaagacc
     7141 tcacccacgt ttacagtgaa attgacgtta tgcacaccgt tctccgtgct caggtcttcc
     7201 acttccaggc agggtatcgc ctgtgtgttg tgcgccttgg ggtagtgctg ctggatgtcg
     7261 aagccgatca tcaagcgcac cacctccccc atctgaaaat cggctaatcg cgctgttttc
     7321 acccggcgcc catccttcaa aatagtaacc ctatcagcga tctggaaaac ctcgtccagg
     7381 cgatgggaga tatagatgat ggctttattc tgggcggtca ggcggtggat aagctccatc
     7441 aggtgctggg tttccaccag gcccagcgcg gcagtgggct catccatgat tagcaagttg
     7501 gatttcagcg agatcgcttt ggcgatttcg actaactgct gttcggcgac ggagagtccc
     7561 ttcacgatag catccggatc gatggtcagg gataggctct ccagcacctc gctcgtcgcc
     7621 ttgcgagctg tgttccaatc gatggtgcct gcagcatttc gagggtaacg tcccaggaaa
     7681 atattctctg ctacggtcag cgtggggacc agcgaaaact cctggtagat cgtcgcgatc
     7741 cccatctgtc gcgcttccat cggattccga aaaatgactt cgcgcccctt gaaaagcatt
     7801 tgtccgctct ccgcttggtg tacgcctgct aggcacttcg ccagagttga cttgccgctt
     7861 ccgttctccc ccagcagggc atgcacttct ccggggtaaa cttcaaacga gacgtcatcc
     7921 agggcttgga cccccgggaa tgctttggag atgttgcgta gctcccagag cggctctccg
     7981 gttggagcca tgcttgtaag ggtatcctcc tcagtgaaga cgctcataca aatctcctat
     8041 gacacctgtg taattcattc ttgccttacc tatgatttta caataaacct cggcaatcag
     8101 aacatccaat cctatccaga gaagaagcct tgcgaagctt gtccgttggc ttgaagacag
     8161 tgcaatacga ccttcgcaag gccattctcc tgaaattagt agccttgcga aggtcgctct
     8221 cagtatccgt caaaccttcg caaggcgatt ttcgtgacta gcttacgggc aggcaaactc
     8281 cttagatggt ttggggtaga ggttttcggg cttgcacagg aagctgccgg cgttgtcctt
     8341 atcggtgttg acggtggggg tctcggtcca tccgccgggg taggtgccat tgagcacgtc
     8401 gagggcgacc tggagagcca ccttgcccat aacgaagggc gtggtgttga ccgtagcagt
     8461 ccactttcca ttgtagatgt cttccagccc agcggtatcg ccgtcgttgc cgaagatgag
     8521 cacatcagat cgacccaagg cttcagcggc tgtggcagcc ccgatggcga tgtagtcgtt
     8581 ggcgcacacc accagattga cttcgggatg agcctggagc aggtccatca tggcggtgtt
     8641 gccggtctcg acgttccagg cggtgggcag gctggcgatg atctccatgc cggggtattc
     8701 tttgaaggca tccaggaagc cgccgatgcg ctcggtggaa tggtagccgg gcaggccttc
     8761 gagcacaccg accttggcgg tgccgccgac catctcagca gcatattttc caaggtcata
     8821 ggtgcccccg cgctggctgt agccgacgac ggcattgacg gggcttggga aggaagcgat
     8881 gtcggagttg acaatcacta cggcgatgcc catgtccact gcctgcttga ccagaggggc
     8941 ggcggcgttc tcgtcgtggg tggagaggat gatggcatcc acgtcctggg tggtgacgtc
     9001 ctggagcatc ttcatctggg tagcgatgtc ggagccggac tgtggggcaa gcatgaaggt
     9061 ttccacgcct gcttcggctg ccagcgcctt gatgccctcg ccgatcgcca tgtagtagtt
     9121 gaactcggtg gcggggggca tgtaggcgat cttgatggga tcgccagctt tgtaggctgt
     9181 tggagtggtg gattgcgatc cctctgccag cggcacaggc ggcatggctc cctctggtgg
     9241 cgtgtaaccc tcctccatgg gagcagcctc gcaggcaaac tccttagatg gtttggggta
     9301 gaggttttcg ggcttgcaca ggaagctgcc ggcgttgtcc ttatcggtgt tgacggtggg
     9361 ggtctcggtc catccgccgg ggtag
//
LOCUS       DBNGSX010000149         3804 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1396361, whole genome shotgun sequence.
ACCESSION   DBNGSX010000149 DBNGSX010000000
VERSION     DBNGSX010000149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1396361"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="AC16CB_04870"
     CDS             complement(<1..266)
                     /locus_tag="AC16CB_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3341836.1"
                     /translation="MSQATYLDKPWIKSYRAGPYKLDASLAPYPREPVFKALDDAAQN
                     YPGQTAVLYLGRSLKYSQLKNQVDRLAAGLVRLGIEKGDRVCLFL"
     gene            complement(287..2173)
                     /locus_tag="AC16CB_04875"
     CDS             complement(287..2173)
                     /locus_tag="AC16CB_04875"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HIR3341837.1"
                     /translation="MSHAYMGKILRVNLTKGEIIEEELREQWVREFIGGAGLATKYLY
                     EEVPKGVDPLGPDNRLIFMTGPLTGTSSASASRYSVVAKSPLTGIWGQANSGGSFGPA
                     LRRSGYDGIIFEGASAIPVYLRIIRGRAELCDASHLWGKTISETEDLIQQSSEQKLTI
                     ASIGPAGENLVRFAAIMNNTHRAAGRCGLGTVMGSKRLKAIACAGNSPVPLANEDLFR
                     EHAKRQIDLLDESMLKVGFDAFGTNMVADMVNVRGGYPTHNWQEGVFEFIDAVNAQGL
                     TDTVFVRGVNCFACPVSCGRGTEIREGKWQGNKGEGPEYETTNTLGALCGVSDLNAIT
                     MANYRCNQFGLDTISAGATIAFAMECYRRGILTPEQTHGLEINFGDADLVVNLVEKIA
                     LREGVGDLLAEGSRGMAQRLGQGSEHFAMNVKGLELPAYDPRAAKLCGLAYVTANRGG
                     DHMTAYIEGPTFVDVPFLLVDDSHIQDPFTANPQEARIVVDLENALTVFDSIGGCKFM
                     GILLTAEDMTNLLANATGWQIGVDDFRKSGERIYNLMRAFCVREGIDRHQDTLPGRLL
                     EDPLPGGPAEGMVIERDSLEKMKDAYYEMRGWDLATGIPTPQKLSELGLEYLIADLWN
                     AKPN"
     gene            complement(2452..3012)
                     /locus_tag="AC16CB_04880"
     CDS             complement(2452..3012)
                     /locus_tag="AC16CB_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341838.1"
                     /translation="MVSTETIERAQDPVEALNASLERAWHSLNQAYDSTAFMPQLPEL
                     FARADLSRPEQAAQAVLVSMLHEVMETAGRFSPWYKQPARAFGLTSVRDPLTQRPRWL
                     LAPEAAQRWRRVLESLSDVISQYAGLIQATILVDDLMSDLPANDPRIIASCTCRPPRT
                     IQLRQSVMDKAEILCQNCSQPFLERG"
     gene            complement(3151..>3804)
                     /locus_tag="AC16CB_04885"
     CDS             complement(3151..>3804)
                     /locus_tag="AC16CB_04885"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HIR3341839.1"
                     /translation="EVTMPCAACFNRHKAALYEIHQQEAHKQQVEKSLDYAYQDRVRV
                     STLTETILNHVGKETIAAKTKKPLKGLRVVCYYGCLLTRPPQVTGAKHPENPTDMDEL
                     LAAAGAEVLDWSYKTVCCGASHSLSRPDIVLKLSGDLIKHARQAGADAMVIACPLCHL
                     NLDARQFQMKLADSMPVLYFTQLLAIALGLPEKEAALHKNLVDPRPLLSAKGMIGTS"
BASE COUNT          760 a         1057 c         1039 g          948 t
ORIGIN      
        1 aggaagaggc aaactcggtc tcctttttcg atccccagcc tgaccagtcc agccgccagc
       61 cggtcgacct ggttctttaa ctggctgtac tttaacgacc tcccgagata tagaactgcg
      121 gtttggccgg ggtaattttg ggctgcgtca tcaagcgcct tgaagacggg ttccctggga
      181 tagggagcca ggctggcatc tagtttatag ggccctgccc ggtagctttt gatccagggt
      241 ttatccagat aggtggcttg actcatggca aaaaatggct ttcccgctaa tttggttttg
      301 cattccagag atctgcgatc agatattcca gccccaattc actcagcttc tggggagtag
      361 ggatgccggt agcgagatcc cagccgcgca tttcgtaata agcgtccttc attttctcta
      421 ggctgtcgcg ctcgatgacc atcccctctg ctggtccgcc cggcaatggg tcttccagca
      481 agcgacctgg tagtgtgtct tgatgccgat cgatcccctc tcgcacacaa aaagcgcgca
      541 tgagattgta gatgcgttcc ccgctcttcc ggaagtcatc taccccaatt tgccatccag
      601 tggcgttggc gagtaagttg gtcatgtctt cagccgtcag aagaataccc atgaacttgc
      661 agccgccgat ggagtcaaaa acagtcaggg cgttttcaag atcaacgaca attctggcct
      721 cttgcgggtt agcagtgaac ggatcttgaa tatggctgtc atcaaccagc aggaagggca
      781 catccacgaa ggtggggcct tcgatgtagg cggtcatgtg gtctccccct cggttggctg
      841 ttacataagc caggccgcag agcttggctg ccctgggatc ataggcaggt agctcgagcc
      901 ctttgacgtt catggcgaag tgttcgctgc cttgacctaa tctttgtgcc atgcccctgc
      961 ttccctccgc cagtagatcg ccgacgcctt cccggagagc gattttttct accaggttca
     1021 ccaccaggtc ggcgtctcca aagttgattt caagaccatg cgtctgttcg ggagtgagga
     1081 tacctctgcg gtagcattcc atggcaaagg cgatggtggc gccagcgctg atggtatcca
     1141 gaccgaactg gttgcaacga tagttcgcca tggtgatggc atttaaatcg gaaacgccgc
     1201 acaaagcacc caatgtattg gtggtttcat attccggccc ttcgcctttg ttcccctgcc
     1261 atttgccttc tctgatttca gtcccgcgac cacacgagac cgggcaagca aagcaattga
     1321 cgccccggac gaatactgtg tccgtcaagc cctgcgcgtt gacagcatca atgaattcaa
     1381 aaaccccttc ctgccagttg tgcgtcgggt atccgcctcg cacgttcacc atatccgcaa
     1441 ccatgtttgt tccaaacgca tcaaaaccga ctttcagcat cgattcgtct aacaaatcga
     1501 tctgtctctt ggcgtgttcc ctaaaaagat cttcgtttgc caggggaaca ggggagttcc
     1561 ccgcgcaggc aatagccttc aaccgcttgg agcccatcac cgtgcctaaa ccacaccgcc
     1621 cggcggcgcg gtgtgtgttg ttcataatag cggcgaagcg gacgaggttt tctccggctg
     1681 gtccaatgga ggcgatagtc aatttttgtt cagagctttg ctgaatcaaa tcctcggttt
     1741 cagagatcgt ctttccccac aggtgggagg cgtcgcacag ctcggcgcga ccgcgtatga
     1801 tccttaagta gacgggaata gcagatgccc cctcgaagat gatgccgtca tatccgcttc
     1861 tcctaagggc agggccaaaa ctgccccctg aattggcttg tccccagatg ccggtcagag
     1921 gagatttcgc caccaccgaa taccggctgg cactggcaga tgatgtgccg gtaaggggac
     1981 cggtcatgaa gatcagccgg ttatcgggcc ctaagggatc aacccctttg gggacctcct
     2041 cgtagaggta tttggttgct aacccggcgc caccaatgaa ctccctgacc cattgctcgc
     2101 gaagctcttc ttcgataatt tctcccttgg tcaggtttac ccgcaagatt ttccccatat
     2161 acgcgtggct cattgtttta tctcctccaa ggcaaattga agatttttta ttatcgctac
     2221 ttacacggtc tattgtgtga acttaaggtt cgtattgagt tggtttagga gattgtgttg
     2281 gacaatatct cagcctagtg tgctcgacga ttcattttgc aatgtggatt ggggcaactt
     2341 tagccagcat gtggcgtgtg tctctctaag ttggcctggc ttatgttggc ggtttgtcct
     2401 ggtaattatg cctatcaccc gcctccgggt ttcgcccagc gtgataataa atcaccctct
     2461 ctctaaaaac ggttgcgagc agttctggca caagatctct gccttgtcca tcactgactg
     2521 gcgaagctgg atcgtgcgcg gcggccgaca ggtgcagctg gcgatgatgc gggggtcgtt
     2581 agcaggcagg tcgctcatca gatcgtccac caggatcgta gcctggatga gacctgcgta
     2641 ctggctgatt acatcgctaa gcgattccaa tactcttcgc cagcgctgag ctgcctcggg
     2701 agccaagagc cagcgcggtc gctgggttag cggatcgcgc accgaagtca acccgaatgc
     2761 ccgcgcgggt tgcttatacc agggtgagaa gcgacccgct gtctccatca cctcatggag
     2821 cattgagacc agcactgcct gagcggcctg ctctggcctg gaaagatctg ccctcgcgaa
     2881 cagctcgggc agctggggca taaaagctgt gctgtcgtat gcctggttca ggctatgcca
     2941 ggcgcgctct agagaggcgt tgagcgcctc aaccggatct tgtgcccgtt cgattgtttc
     3001 cgttgaaacc atgcgaagac ctcctctggt tactcctcaa acctgggtgt gatctttcgg
     3061 gtcttggagc gtgtagcctc tctgagcgag gtggtgttaa ggaaggagta cttcgatgcc
     3121 tctccgggat ggctcagccg cctggagaat tcacgatgtt ccgatcatcc ctttggcaga
     3181 taacagcggc cttggatcta ccaggttttt atgcagagcc gcttccttct caggtaatcc
     3241 aagtgcaatg gctagcaatt gggtgaagta cagcactggc atcgaatccg ccagtttcat
     3301 ctggaactgg cgtgcgtcca ggttcaggtg gcacagcggg caagcgatga ccatggcatc
     3361 tgctcccgcc tggcgggcgt gcttgatcaa atctccgcta agcttgagca cgatatccgg
     3421 acggctgagc gagtgcgacg caccgcagca gacggtcttg tacgaccagt ccagcacctc
     3481 cgccccggct gccgccagca gctcgtccat gtcggtggga ttctccgggt gcttggctcc
     3541 ggtcacttgc ggtgggcggg tgagcaagca gccgtaataa cacactaccc gcagaccttt
     3601 aagcggtttt ttggtttttg ctgcgattgt ctccttgcct acatgattga ggattgtttc
     3661 cgtcagcgtg ctgacgcgca ctcgatcttg ataggcgtag tccagcgatt tttctacctg
     3721 ctgcttgtgc gcctcctgct ggtggatttc gtacagcgca gctttgtggc ggttgaagca
     3781 ggctgcgcaa ggcatggtca cttc
//
LOCUS       DBNGSX010000150         4098 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_80814, whole genome shotgun sequence.
ACCESSION   DBNGSX010000150 DBNGSX010000000
VERSION     DBNGSX010000150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_80814"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..1123)
                     /locus_tag="AC16CB_04890"
     CDS             complement(188..1123)
                     /locus_tag="AC16CB_04890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3341840.1"
                     /translation="MKIVEYDDVDPLKVLCVNQLALEFALSPEKVAHIRQTDPRPFPC
                     LAIYAVEDGVVLGQVGIFRLPMISTEGREDVGGIWAVSTHPQYAGHGVASRLLEEAHT
                     RMRDAGLRFSTLGTDRYRVAYNLYRQHGYEETNVWATALARWETAHQPTRLRAQPPGP
                     EGHDIVEQVYKNIASDYLGFAWRHTPFVRLHDTVSLENIWILWEHQQPVGYALVQVDK
                     AMLKITNLLLRDGINAAEAIAAVVVRTKTPYIQVRVSRPSEVASLQRAGYQVAHPTWD
                     AFLVKSLVPEVTADDARRLFGIGTDRFLISWLDVT"
     gene            complement(1110..2135)
                     /locus_tag="AC16CB_04895"
     CDS             complement(1110..2135)
                     /locus_tag="AC16CB_04895"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HIR3341841.1"
                     /translation="MDKTKQQITRIAIVGTGHVGATCAYALQLLGHASEIVLINAHRE
                     RAEGEAMDISHGSLFARPVRIWAGDFSDCRDADIVVLTAGASQKPGETRLDLLRRNAA
                     IYQNILPQVVQYNRNAILIVVANPVDVLTYITLQITQLPARQVIGSGTILDTSRFRCL
                     LSQHFGIDPRSIHAYILGEHGDSQVPVWSLANIAGMRLDEYARLNNKPLDDYSRHEIA
                     QNTRQAAYEIIRRKGATYYAIAAGVVRIIEAIVRDENSVLTLSSLIQGKYGLNDVCLS
                     LPSIVNRQGILETLELPLSPEEQTGLEKSARVLREAIDSLQEYTSPNLVFNIPSRYSV
                     GCTYENR"
     gene            complement(2223..2600)
                     /locus_tag="AC16CB_04900"
     CDS             complement(2223..2600)
                     /locus_tag="AC16CB_04900"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3341842.1"
                     /translation="MKPLTLDLFNSWLETYGKASRENDLQASADLFSLDAKYYETPFD
                     EPMIGRDAIYQYWSQGAQRFKDKESSYEIISMEDNLGIARWQSQFTAINSGKRLRLDC
                     LFLVEFDENGLCSVFREWWHISK"
     gene            complement(2597..3475)
                     /locus_tag="AC16CB_04905"
     CDS             complement(2597..3475)
                     /locus_tag="AC16CB_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012995.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HIR3341843.1"
                     /translation="MAYPNLSDTLVKDILDYLECPKKAPTLRYLNHLIKAYLRKVPWE
                     SVSRIVKRHATQETEDCPRWPEEFWQETMQLGFGGTCYESSLAFYSLLMALGYEGYLT
                     VNDMGASRGCHAAIVLLLNKHKYLVDITMPIHVAVRIDPQKIVRRNTAFQNYTIRPVQ
                     ENKFEVERSHHPNRNAFTMIDIPVSLQDYQEIVKNDYRETGFFLRSVVMVRVMGDKTW
                     RFFSDHKPYKLESFDRAGKREIFLEPETLPHILAELFQMPEDQISAALSWISTPSIMP
                     RNEIISLPMVLEGSAQ"
     gene            complement(3509..>4098)
                     /locus_tag="AC16CB_04910"
     CDS             complement(3509..>4098)
                     /locus_tag="AC16CB_04910"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HIR3341844.1"
                     /translation="EFTIMLFEVHQNLANLVAELLDLTGVERMMDLGGNSGVVSMALL
                     RKYPELTSTVVDIENVCFAGREIAEREGFSDRITYHTADFEDDEFPSGFDLVLQCDVA
                     QYGSELINKLYRSLKPGGRLIYVDHFSPAENLAPITRLEWTFLDSLHDPNFSFPTLDE
                     VKSRLAQTGFEVSDELHTLGRGLIVLQAWKRDLAH"
BASE COUNT         1016 a         1066 c          958 g         1058 t
ORIGIN      
        1 ccaataaccc tgcaccaatg ttttcctttt attgtcttta gttctattta ataactccgt
       61 cgtctaccag ataggctggc gattttgcca tcggctgagt agggaccgag cagccacgcg
      121 cgtgcgctgt ttggcggtct gaggatcgac gtggcgccaa accccgtagg tgaatttgtc
      181 ctggttttca cgtcacatcc agccacgaga tcaagaagcg gtctgtgcca atgccgaaca
      241 gccgccgggc atcatccgca gtgacctcag gcacaagcga tttgaccagg aaagcgtccc
      301 aggttgggtg cgcaacctga tatcctgcgc gttgcaggct ggcaacctcg cttgggcggc
      361 tgaccctgac ctggatataa ggggtctttg ttctcaccac cacagccgcg atcgcttcag
      421 ctgcgttgat gccatcgcgc agcaataaat tggtgatctt caacatggcc ttgtcaactt
      481 ggaccagcgc atacccaacc ggctgttggt gttcccaaag gatccaaata ttttcaaggc
      541 taactgtgtc atgcaggcgc acaaagggag tgtgccgcca ggcaaagccc aggtagtcac
      601 tggcaatatt cttatatacc tgctcaacaa tatcgtggcc ttcaggccct ggaggttggg
      661 cgcgcaggcg tgtcggctgg tgggctgttt cccagcgcgc aagcgcggta gcccaaacat
      721 ttgtctcctc gtacccgtgc tggcgataca ggttataagc cacgcgatac cggtcggtac
      781 ccagtgtgga aaagcgtaag ccagcatcgc gcattcgagt atgcgcctct tccagtaatc
      841 gggaggcaac tccatgccca gcgtattgcg gatgtgttga gacagcccag atacccccca
      901 cgtcttccct tccttcggtt gaaatcatcg gcaggcgaaa aatccccacc tgacccaaga
      961 caacgccatc ttcaacagca taaattgcca ggcaaggaaa tgggcgcgga tctgtttggc
     1021 ggatatgcgc caccttttcg ggtgaaagcg caaattcaag cgccaattgg tttacacaca
     1081 ggacctttaa tgggtccacg tcgtcatatt caacgatttt catacgtaca tccgacagaa
     1141 tacctggaag gtatattaaa aacaaggtta ggtgaggtat attcctgaag cgaatcaatt
     1201 gcctcgcgca ggactctggc tgatttttca agacccgttt gttcttcagg agagaggggc
     1261 aactccaacg tttctaggat gccttgccga ttcacaatgc tgggcaggct gaggcaaaca
     1321 tcattcaatc catacttgcc ttgaatcagg cttgataagg ttagaacact gttctcatcg
     1381 cgcacgattg cctcaataat ccttactaca cccgcagcaa ttgcataata cgtagcgcct
     1441 tttctgcgga taatttcata cgccgcttgg cgcgtgtttt gggctatctc atgccttgaa
     1501 taatcatcaa gaggcttatt attcaagcgg gcatattcat ctaatcgcat gccggcaata
     1561 ttagcgagac tccataccgg aacttgcgag tcgccatgtt ctccgagaat gtaagcgtga
     1621 atgctgcgcg gatcgattcc aaaatgctgg ctgagtaaac agcggaagcg ggatgtatcc
     1681 agaatcgtcc cagagccaat cacctggcgc gccggcaatt gagttatttg caatgtaata
     1741 taggtaagca catcgaccgg attcgccact acgatcaaaa tagcattcct gttatattgc
     1801 accacctgag gcaggatatt ctgatagatc gcagcattgc gtcttagcaa atccaacctg
     1861 gtctcacctg gtttttgcga agctcccgcc gtcaacacca caatatccgc gtctctgcaa
     1921 tcagagaaat cacccgccca tatccgcacc ggccgagcaa acaaggatcc atgactgata
     1981 tccatggctt caccttcagc gcgttcgcgg tgagcgttga tcaagacgat ttcagatgca
     2041 tgccccaaga gctgcagcgc atacgcgcat gttgcaccta catggcctgt tccgacaatc
     2101 gctatgcggg ttatttgttg ttttgtttta tccatgttta cctctgcaaa accgaaacta
     2161 tttagcgttg atgtgtgttt ctataattca aaaaaattgt ctcgctaccg gtcatgggca
     2221 tactatttcg atatatgcca ccattcacga aacacgctac aaagtccatt ttcatcaaac
     2281 tcaaccagga acagacagtc cagcctgagg cgtttcccag aattgatggc ggtgaattgg
     2341 gattgccagc gtgcaatccc caaattgtct tccatcgaaa taatctcata agaagattct
     2401 ttgtctttaa acctctgcgc gccttggctc caatactggt aaatcgcgtc gcgtccaatc
     2461 ataggttcat cgaagggcgt ttcataatat ttggcatcaa gcgagaataa atcagccgag
     2521 gcttgtagat cgttttctct gctggctttg ccatatgttt caagccagga gttgaacaaa
     2581 tccaatgtca gtggtttcat tgcgcgcttc cttccagaac catgggaaga cttatgattt
     2641 cattacgagg cataattgac ggggttgata tccatgatag cgcagcagaa atctgatctt
     2701 caggcatttg gaataattct gccaatatgt gaggcagagt ttcgggctcc agaaatattt
     2761 cccttttacc tgctcgatcg aagctctcca gtttgtaggg cttatgatcg ctaaagaatc
     2821 gccaggtttt gtcgcccatc accctgacca tgacaacgct gcgtagaaag aaacctgttt
     2881 cccgataatc atttttcaca atttcttgat agtcctgcaa actgacagga atatcaatca
     2941 tggtgaaggc gtttctattc gggtgatggg atcgctccac ttcaaatttg ttttcctgca
     3001 cgggtcgaat cgtgtaattt tggaatgcgg tgttccgcct cactatcttt tgcggatcga
     3061 tgcggacggc aacgtgtatg ggcatggtga tatccaccag atatttatgc ttgtttagca
     3121 atagaacaat ggcggcatga cacccgcgcg aggctcccat gtcattgacg gtcagataac
     3181 cttcatagcc cagcgccatt aacaggctgt aaaaagccag gcttgactcg tagcacgtcc
     3241 cgccaaagcc aagctgcatg gtttcctgcc agaattcttc aggccagcgc ggacaatctt
     3301 ctgtttcctg cgttgcatga cgttttacga tacgcgagac gctttcccac ggaaccttgc
     3361 ggagataagc cttaatgaga tggttcaaat atcgcaaggt cggcgctttt tttggacatt
     3421 ctaggtagtc caatatatct tttaccaggg tatcgctcaa atttgggtat gccattgact
     3481 gccgctcctt gcttttccac attgaacatc aatgtgctaa atcccttttc cacgcctgga
     3541 gcactatcag ccctctgccc agtgtgtgga gttcatctga cacctcaaag cctgtttgag
     3601 ccaatcgtga tttgacctca tcaagtgttg ggaaactgaa attggggtcg tgcaaagaat
     3661 ccaggaatgt ccactccagc cttgtaatag gcgcgagatt ttccgcaggg gaaaagtgat
     3721 ccacatagat caatcgtccg cccggtttca aagaccggta caatttattg atcagttcgg
     3781 aaccatactg ggctacgtca cactgcaata ccaggtcgaa accggatggg aattcgtcat
     3841 cctcaaaatc agcagtgtga tacgtaatgc ggtcagaaaa tccctccctc tcggcaattt
     3901 cacgcccagc aaaacagaca ttctcaatat ccaccaccgt ggaggtgagt tcgggatact
     3961 ttcgcaacaa cgccatggag accacccctg aattaccacc caagtccatc atgcgttcga
     4021 cacccgttag atccagcagt tctgcgacca ggtttgccag gttttggtgg acttcgaaga
     4081 gcataatcgt gaattcac
//
LOCUS       DBNGSX010000151         9595 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_565429, whole genome shotgun sequence.
ACCESSION   DBNGSX010000151 DBNGSX010000000
VERSION     DBNGSX010000151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9595)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9595
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_565429"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..497)
                     /locus_tag="AC16CB_04915"
     CDS             complement(189..497)
                     /locus_tag="AC16CB_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341845.1"
                     /translation="MKKIILLLTGMALTLAACQGQAVSPQPAPTEAATPSLVPTIQAT
                     LAEATPTQPEAPAGCTAISPKPTPGPTETSLFPPVSPDDWVKGPADASITLTEYSDFQ
                     "
     gene            complement(672..5984)
                     /locus_tag="AC16CB_04920"
     CDS             complement(672..5984)
                     /locus_tag="AC16CB_04920"
                     /inference="COORDINATES: protein motif:HMM:NF044287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nSTAND1 domain-containing NTPase"
                     /protein_id="HIR3341846.1"
                     /translation="MLEVWLLGKFEVRLDGVPVHLPSRSEQSLLAYLLLNAGTAYRRE
                     KLAGLFWPESDESNARGYLRIALWRIRKAFKDLSPHSPDFFVTDKLTIAFNCELECWS
                     DVAVLKNGDGQSIQGLVEATSVYQGELLPGFYDEWVVLERESLRAAFERKIYTLVERL
                     LSERRWQEAIEQTERWISLGDTPEPAYRGLMIAHAGLGEASAAITAYQRCVEALKKDL
                     DIQPSTETRALYEKIRSGQEIYPALRSRHIRGFELHEKIGEGSFGVVYRALQPSVGRE
                     VAVKFILPQYANEPEFIRRFETEAQVVARLEHPRIVPLYDYWREPDGAYLVMRLLNGG
                     SLEAVLDKGPMEIIAATDLVDQIASALALAHQHGVIHRDIKPANILFDESGNAYLSDF
                     GIANVLGGNRHQTQAGTLFGTPVYMSPEQIRDQPVTPQSDIYSLGIILFQLLTGEAPF
                     LDEALPALIEKHLHQLLPSVRHRRPDLLDEVDQVIQRATAKDPAGRFPDALSLAAAFR
                     QALSGLQLQDPAEAVPPKEFVAELTNPYKGLRAFQEADAADFFGREAMLERLLARLAE
                     DGPACRFLAVVGPSGSGKSSLVKAGLIPALRRGALPSSKRWFVVEMIPGSHPFEELEI
                     SLLRIASNPAVSLIEHLRRDQRGLLRAARLALPSEEGHLLLVIDQFEEIFTLAEDEAE
                     ARQFMESLYVAVSEPRSPIHVVISLRADFFDRPLMNKDLSTLLQDRTEVVVPLTVSEL
                     ESAIRSPAHRVGVEIEPGLVTAMVAEVVEQPGALPLLQFALTELFEQRTSQRMTLEDY
                     NAIGGVEGVLDQRAEDVFSRLSEAGKATARQIFLRLVTLGEGVEDTRRRVLRSELEAL
                     SPAPSPLQGKGNQLQGSVESVIDVFGRARLLSFDRDAATRSPTVEIAHEALLREWRRL
                     RGWRDDGRADVRMQRALGNAAAEWIKASQDASFLLHGARLEQFEGWAGETNLALTGDE
                     RLFLETSLAEREAQRELEVSRQRREAALELRVRRFLRALVVVLLLATLGSSALAWTAR
                     RAQIFAEGQSQARATQQAVAIQERDKAQQQTDLASSRELAVVAINNLEADPERSILLA
                     LNALRTAYTREAEASLHQAVQASRVRMALTGHAGAARSVEYSPEGTTIATASDADEVT
                     VWEAVSGQKLFNLTGRVARYSPDSSRLATGSQDGTVTIWDLGTRQKILTLRGHSKGIG
                     DVHFSPDGRLLVSSSLDDTFIVWDTQTGEELFTSSARTGGYDTLYNVGFSPDGRSLIS
                     TDFYSTDEIEMSIWGVEQDWDLLNQTPGYAIFVISPDGRWMAASVGELLSDIFLWDLS
                     KLSGPNPKGLDLSTVESILVPAAHDSVIVGLAFSLDGTHIASASLDGTAKLWSLSPGG
                     VELLMTLSGHTKEVVDVTFSPDGTRLATASIDGSVRIWDITPDGASEWFALPAHSEAI
                     FRFGLAADGKYLATASPDGSAKVWNLASRQELIAIDQHGGPLFGVAISPDGSLLATAG
                     YDNIAKIWKLDLSPGAASAELLHALTGHAAGPPLGGTFPGLSSVAFSPDSTMLATGGV
                     DNYAKVWDVKTGQELLSVQVDPDGSGVTRLAFNPDGLLLATASDDVPDVTLAKIWDVT
                     SGREISTFSGHSQTGRIWGLAFSPDGKRVASSGGAGVLKIWDTSSGEELLNIAGHSSV
                     VTGVDFSPDGKYLASTSLDGTARVWDASSGEELRLYTNPSGPLYGVAFTPDGKNVIAT
                     GAGLVYGYIFDTQDLIRLATSRLTRWFTLDECRQYLHRDDCPSR"
     gene            6218..6406
                     /locus_tag="AC16CB_04925"
     CDS             6218..6406
                     /locus_tag="AC16CB_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341847.1"
                     /translation="MYNDVTQRNYGKARMEILQKEAQSRRSVAAYEAGEAEKSPPIIK
                     LQKSGFQRLLSWLLAGLR"
     gene            complement(6418..7674)
                     /locus_tag="AC16CB_04930"
     CDS             complement(6418..7674)
                     /locus_tag="AC16CB_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341848.1"
                     /translation="MRLDRIASIVAFLALVALLLGGSSLPAGDKIERVRAFTREIEFD
                     YVAWTLNALEVKLSQVALGTSAYLSNDARHQQVLDYLDLVVQIQQSEAQLEEFFASPE
                     IANPQEASAPLRQKLEEMYARRSQLGPLAESILQEQLSNTAAEIGITLGGQPIPPVLY
                     HSTPLPRALIVSPREVIRQDNNISINPDLTIDQRVTLEERVDQALNVSSLVVEIGGIG
                     VYPTMVMQTSDLNWMAEVVAHEWIHNFLTLRPLGLNYETSPELRTINETVASIAGKEI
                     GRILVARYYPELAPPPPPEAPAESPPPLLPVEPPPFDFRAEMRITRVTVDQLLAEGKI
                     EEAEDYMEQRRLIFWDNGYHIRKINQAYFAFYGAYADQPGGAAGEDPVGAAVRLLRSR
                     SATLAEFLNRVSWMYSYDQLQRAVGE"
     gene            7768..8718
                     /locus_tag="AC16CB_04935"
     CDS             7768..8718
                     /locus_tag="AC16CB_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3341849.1"
                     /translation="MNFLITGAAGFLGSALANQLGREGHQVRGLDDLSTGDPQALSPD
                     VLFTRGDVNDRPKLWTLLQDVDCVYHLAARVAVPESLLYPREYNAVNVGGTVSLMEAM
                     RDVGVRRVVFISSGAVYGDQGEQPLREDAIPNPRSPYAVSKLAAEYYVRTIGDQWGIE
                     TVCLRVFNAYGPGQHLPASHPPVIPYFLKQAVRGGTLVLHGDGTQTRDYVYVSDVVRC
                     MVSAATAPNINKMIINVGSGTETSVRELVRLVRKVVTGGKPELIFNPRTHPGVSRMCA
                     DLTLAREKLGFQPRVSLVDGLSMTLERDLRFKKEPTRPLR"
     gene            8722..9327
                     /locus_tag="AC16CB_04940"
     CDS             8722..9327
                     /locus_tag="AC16CB_04940"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HIR3341850.1"
                     /translation="MPDPEQLERPFFSRSTLQVARELLGMRLVRQERDVRLVGVISEV
                     EAYRGEEDQGCHARAGRTPRTRVMYGPPGHAYVYFTYGMHWMLNFVTEAEGYPAAVLI
                     RAILPVEGLDVIAARRDGQPFAHWTDGPAKLCQALGIDRRHNGLDLCAPQSEIFVQVG
                     ESIPDSAVTIGPRVGLNNVPEPWKSIAWRFRIQSADFTGVY"
     gene            9432..>9595
                     /locus_tag="AC16CB_04945"
     CDS             9432..>9595
                     /locus_tag="AC16CB_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3341851.1"
                     /translation="MKLLEGKTALVFGLANERSIAWGITKAFHEHGATLGISYAGEVL
                     ERRVRPLAAE"
BASE COUNT         1960 a         2714 c         2848 g         2073 t
ORIGIN      
        1 cgttcaaaga gcagatcgtg catctcccaa aatttacctt gcatcccggc agcctcggca
       61 gcttgagccg atagggcggc cttatcatgg atggagatca acgggaaatg gcgatagacg
      121 atgcggatat ctttgggtga ctcttcttgt agctgcgcca acacgggcgc cagatcagca
      181 cagcctggtc attggaaatc gctatactca gtcagcgtga tggaggcatc ggcagggccc
      241 ttgacccaat catctgggct gacgggtggg aatagcgagg tttccgtcgg gccaggcgtg
      301 ggcttggggc tgatcgcggt gcagccagcc ggggcttctg gctgagtggg tgtggcttct
      361 gccagggtgg cctggatggt tgggaccagg gagggcgtag ctgcttcggt cggtgcgggc
      421 tgaggcgaaa ctgcctgccc ctggcaagcg gcgagggtta gcgccatgcc ggttaacaat
      481 aggatgattt tcttcatagg tagtcttgat ccagttgtgt gaatttcgat ctgcccattg
      541 tatcctattc tggagaatat ttttaaaaaa ccacttccct ccaccataga atgaggaggg
      601 aagcggcaag cgggatgggg gataccagat gattgcagat tcgtccctac cagaaaccct
      661 tgagggatcg atcagcgtga ggggcaatcg tcccgatgaa gatactggcg gcactcgtcg
      721 agcgtgaacc agcgcgtcag acgcgaagtg gccagacgga tcaagtcctg cgtgtcgaaa
      781 atatagccgt acacaagacc tgcgccggta gcgatgacat ttttcccgtc tggggtgaaa
      841 gccacgccgt agaggggccc gcttgggttg gtatataacc gcagctcctc ccctgaagaa
      901 gcgtcccaca ccctggcggt gccatccaag cttgtcgatg ccaggtattt cccgtccggg
      961 ctgaaatcca cacctgtgac cacggagcta tgcccggcga tgttcagcag ttcttcgccg
     1021 ctcgatgtat cccaaatttt taacacgcct gcccctccac ttgaagctac gcgtttccca
     1081 tccgggctga aagccaggcc ccagatacgc ccggtctggc tgtgccctga aaaggtggag
     1141 atctcacggc cactggttac gtcccagatt ttggccagcg tgacatcagg tacatcgtca
     1201 ctggctgtcg ctaagaggag accgtccggg ttaaaagcca gccgggtgac cccactgcca
     1261 tccggatcga cctggacgga gagcagctct tgaccggtct tcacgtccca caccttcgca
     1321 taattatcca cgccgccggt cgccagcatc gtgctatccg ggctgaaggc gacagagctc
     1381 agcccgggaa aagtgccccc gagcggcggc ccagccgcat gtccggttag agcgtgtaac
     1441 agctcggcgg aggctgctcc cggggagaga tccaatttcc agattttggc gatattgtcg
     1501 tatccggcgg ttgccagcag gcttccatcc gggctgatgg ccacgccaaa tagcgggccg
     1561 ccatgctggt cgatggcgat cagctcctgt ctcgaagcca ggttccacac tttagcgctt
     1621 ccatccgggc tggcagtagc caggtatttg ccatctgcgg cgaggccgaa gcgaaagata
     1681 gcttcggagt gggccggcaa agcgaaccac tccgaagctc cgtctggagt gatgtcccag
     1741 atgcgaacgc tgccatcaat gctggcggtg gccagtcgtg ttccatccgg gctgaaggtg
     1801 acgtcgacaa cttccttggt gtggccggat agggtcatga gcagttcaac ccctcccggg
     1861 gaaaggctcc acagtttagc agtgccgtcc aaactcgccg acgctatgtg tgttccatcg
     1921 aggctaaaag caagaccgac aattacagag tcgtgtgccg caggaacaag gatggattcc
     1981 acggttgaca ggtccagacc tttcgggttt gggccggata acttcgagag atcccacaaa
     2041 aaaatatctg atagtagctc cccaaccgac gctgccatcc acctgccatc cgggctaatg
     2101 acgaagatcg catagcctgg agtttggttg agcaaatccc agtcctgctc caccccccaa
     2161 atactcatct cgatctcgtc agtgctgtaa aaatctgtgg agattaacga cctgccatcc
     2221 gggctgaagc ccacattata aagcgtatca tacccgcccg tcctggcgct tgaagtaaac
     2281 agttcctcac cggtctgtgt atcccacacg atgaaggtgt catccaggct ggagctcacc
     2341 agcaacctgc catccgggct gaagtgcacg tctccgatac ccttgctatg gccgcggagg
     2401 gtcaagatct tctgccgtgt ccctagatcc caaatcgtga ccgtaccgtc ctgacttccg
     2461 gtagccaggc gcgagctatc cgggctgtag cgggcaaccc tcccggtcag gttgaacagc
     2521 ttctgcccgc tgacagcctc ccagacggtc acctcgtccg catcgctggc tgtggcgata
     2581 gtcgtcccct ccgggctgta ctcgaccgag cgggcggcac cggcatgccc ggtcaatgcc
     2641 attctcacac gcgaagcctg gacggcttgg tgcagggaag cttcagcttc gcgtgtatac
     2701 gctgtgcgta gagcgttcag ggcgagcaag atgctgcgtt cagggtcggc ctccaggtta
     2761 ttgatcgcta ccacggccag ctctcgcgag ctagccaggt cggtctgctg ttgggcctta
     2821 tctctttcct ggatggcgac tgcctgctgg gtggcgcggg cttgcgattg cccctcggcg
     2881 aagatttgtg ccctgcgggc ggtccacgcc agtgctgaag atcccagggt ggccaataac
     2941 aagacgacca ccaaggcgcg cagaaagcgg cggacacgca gctccagggc ggcctcgcgc
     3001 ctctggcggg atacttccag ctccctttgt gcctcccttt cagccaggct ggtctccagg
     3061 aagaggcgct cgtctcccgt gagggccagg ttggtctccc cagcccagcc ctcgaattgc
     3121 tccaggcggg cgccgtgcag caagaagctg gcatcctggc tggccttgat ccactcggcg
     3181 gcggcgttcc ccagggcgcg ctgcatgcgc acgtccgccc ggccatcgtc ccgccaccca
     3241 cgcagccgcc gccattccct gagcagggct tcgtgggcga tctccaccgt cgggctgcgg
     3301 gtcgcggcgt cgcggtcgaa ggagagcagg cgcgctctcc cgaaaacgtc gatgacggac
     3361 tcgaccgacc cctgtaactg attccccttg ccttggaggg gagaaggggc aggggacagg
     3421 gcttccagct cggagcgcaa gaccctgcgg cgggtatctt cgacgccttc acccagcgtg
     3481 accaggcgca ggaagatctg ccgcgcggtg gcttttccgg cttcactcag ccgggagaat
     3541 acgtcctcgg cgcgttgatc caggacgccc tccacgccac cgatggcatt gtaatcctcc
     3601 agggtcattc gctgggaggt acgctgctcg aacaattcgg tcaatgcaaa ttgaagcagt
     3661 ggaagagcgc ctggttgttc caccacctcg gccaccatgg cggtcaccag gccaggctcg
     3721 atctctacac ccacccggtg cgctggggag cgaatggcgc tttccagctc gctgacggtg
     3781 agcgggacca ccacctcggt gcggtcttgc aatagggtgc tgaggtcctt gttcatcaag
     3841 gggcggtcga agaagtctgc cctcaggctg atcacgacgt gaattgggct gcgcggctca
     3901 ctgacggcga catacaggct ttccataaat tgcctggctt cagcctcgtc ttctgccagg
     3961 gtgaagattt cttcgaactg gtcgataacc agcagcaggt ggccctcctc ggatggcagc
     4021 gccagccgcg ccgctcgcag aagcccgcgc tggtcccggc ggagatgctc gatcaggctg
     4081 accgccggat tggaggcgat gcgcagcagg ctaatctcca gctcttcgaa gggatggctg
     4141 ccggggatca tctccacgac gaaccatctc ttggagctgg gcagcgcccc acgtcgcagt
     4201 gccgggatca agccggcttt caccagggag gatttgccgc tcccgctggg cccgaccacg
     4261 gccaggaaac ggcatgccgg accgtcttcg gcaaggcggg cgagcaaccg ctccagcatg
     4321 gcttcccggc cgaagaagtc ggctgcatcc gcctcttgaa aggcgcgcag gcccttgtag
     4381 gggtttgtca gttccgcaac aaactctttg ggaggtacag cttctgccgg gtcttgaagc
     4441 tgcaatccgc tcaacgcctg tcggaacgcc gcggccaatg acagagcatc tgggaagcga
     4501 ccagcgggat cttttgccgt ggcgcgctgg atcacctgat ccacctcatc cagcaggtcg
     4561 ggccgacgat ggcgcacgga tggcagcagt tggtgaaggt gtttttcgat cagcgctggc
     4621 aaggcttcat caaggaaagg cgcttcccca gtcagtaatt ggaacaggat gatgcctaag
     4681 ctgtagatat ccgattgggg tgtgaccggc tggtcccgga tctgttccgg agacatatat
     4741 accggggtac cgaatagcgt cccggcctgg gtctggtgtc tattccctcc cagcacattg
     4801 gcgatgccga aatcggacag ataggcgttc ccgctctcgt cgaaaaggat attggctggc
     4861 ttgatatctc gatggatcac tccatgttgg tgtgccaggg cgagcgctga cgcgatctgg
     4921 tccaccaggt cagtggcagc gattatctcc atcggtcctt tatccagcac ggcctccagg
     4981 ctacctccat tgagcaggcg catgaccaga tacgcgccgt ccggctcgcg ccagtagtca
     5041 tataatggga cgatgcgcgg gtgttccagg cgggcgacga cctgcgcctc tgtctcgaag
     5101 cggcggatga attcaggttc gttggcgtac tggggcagga tgaacttaac tgctacttct
     5161 ctgccgactg atggctgcaa agcgcgatag acaaccccaa agctaccctc tccgatcttc
     5221 tcgtgtaatt caaaacctcg gatgtgtcgc gatcttaggg ctgggtagat ctcctggcca
     5281 gagcggattt tctcatacaa ggccctggtt tcagtcgatg gctggatgtc aaggtctttc
     5341 ttcaaggctt cgacgcagcg ctgataggcg gtaatggcag cagatgcttc ccccaatccg
     5401 gcatgggcga tcattaagcc cctataggct ggctctgggg tatcgcccaa ggatatccat
     5461 cgctctgtct gctcaatcgc ctcctgccag cgtctctcgg agagcagcct ttctaccaga
     5521 gtatatatct ttcgctcaaa ggctgctcgc aggctctccc tttctaacac cacccactcg
     5581 tcataaaagc cggggagcag ctcgccttgg tacacgctcg ttgcctccac cagaccttga
     5641 atcgactgcc cgtcaccgtt tttcagaacg gcaacgtctg accagcattc gagttcgcag
     5701 ttgaaggcga tggttaattt gtcggttacg aagaaatcgg gagagtgggg agaaaggtcc
     5761 ttgaacgcct ttcgaattcg ccacaatgca atccggagat atcccctggc gttcgactca
     5821 tctgattcag gccagaacaa gccagccagc ttttcgcgcc ggtaggccgt cccggcatta
     5881 agcagcaaat acgccagcag agactgctcg gagcgtgatg ggagatgaac agggacgcca
     5941 tccagccgaa cttcaaattt gccaagaagc catacttcga gcacttgttc ctccaaactt
     6001 tataaaaatt ggctgtcgag gggatgatga cgtgaagctc atcgcgccat aattaagaac
     6061 gctacttgga gtaattctat catagttggg aaaatcatac gatgaatata tggcgataaa
     6121 tacgatctat atacgtcgtt tatttatact cttgaacaga tatgagaaac cgaatctaga
     6181 gtatagatcg aaattagtca ggtgaaatga ggttgatatg tataacgatg tgacgcaaag
     6241 gaactacggc aaagcccgca tggagatcct tcagaaagag gctcaaagca gaagatcagt
     6301 ggcggcttat gaagccgggg aagctgagaa atctccccca atcatcaaat tgcagaagag
     6361 tggcttccag cgcctcctga gctggctgct cgctggtttg agatagtgat gtgtggatta
     6421 ttcccccacc gctcgctgca attggtcgta tgaatacatc cacgagacgc ggttgaggaa
     6481 ttcggctagg gtggcgctcc gggagcgcaa caggcgcacc gccgcgccca ccggatcttc
     6541 gccggcagca ccgccgggct ggtctgcgta ggcaccatag aaggcgaaat aagcctggtt
     6601 gatcttgcga atatggtagc cattatccca gaagatcagg cggcgctgtt ccatgtagtc
     6661 ttctgcctcc tcaatcttcc cctctgccag cagctgatcc actgtcacgc gtgtgatgcg
     6721 catctcggcg cggaagtcga agggtggcgg ctctacaggg agaagcggag gcggggattc
     6781 ggctggcgct tccggcggtg gtggcggggc taactccggg taatagcgcg ctaccaatat
     6841 tcgcccgatc tctttgccgg caatggaggc gacagtttcg ttgatggttc gtaattctgg
     6901 gctggtctca taattcagcc cgagtgggcg cagggtgagg aagttgtgga tccactcgtg
     6961 tgctaccacc tccgccatcc agttgagatc gctggtctgc atgaccatgg ttgggtagac
     7021 accgatgccg ccgatctcaa ccaccagcga agatacgttc aaggcctggt caacgcgctc
     7081 ttctagcgtc acccgctggt cgatggtcag gtcagggttg atagagatgt tgttatcctg
     7141 gcggattacc tcgcgcggcg agacgatcaa tgcccgcggc agcggggtgc tgtgataaag
     7201 gaccgggggt atgggctgcc cgccaagcgt tatcccgatc tcggctgccg tgttggaaag
     7261 ttgttcctgt aagatcgact cggctaaggg acccagctgt gagcggcgcg catacatctc
     7321 ttccagtttc tgtcgcagcg gggcgctggc ttcttggggg ttggcaatct ctggactcgc
     7381 gaagaattcc tccaattgtg cttcactttg ctgaatttgc acaaccagat ccaggtaatc
     7441 gagcacttgt tggtggcggg cgtcgttgga cagataggct gaggtgccca gggcgacctg
     7501 gctcaacttt acttccagcg cgtttagcgt ccaggcgaca taatcgaatt caatctccct
     7561 ggtgaaagcg cgcacgcgct cgatcttatc cccggcgggc aggctgctgc cgccaagcaa
     7621 aagtgctacc agggcaagaa aggctacgat tgaagctata cgatccaagc gcatgggggc
     7681 gatgataacg ccatcatagc agtatgtcaa ttatggtaaa ttgggagagc aggcatccat
     7741 gcctttcatg ctataatcac ccccacgatg aatttcctga tcaccggcgc ggccggattt
     7801 ttaggctcag cgctcgctaa ccaattaggg cgcgaaggcc accaggtgcg cggcctcgac
     7861 gatctttcca caggcgatcc gcaggcgctc tccccggatg tgctctttac gcgcggcgac
     7921 gtgaacgacc gccccaagct ttggacgctc ctccaggatg tagattgtgt ttaccatcta
     7981 gccgcgcgtg ttgcggtgcc cgaatcgctg ttgtacccgc gcgaatataa cgctgtcaat
     8041 gtgggtggca cggtcagttt gatggaggcc atgcgcgatg tgggcgtgcg ccgggtggtg
     8101 ttcatctctt caggcgcggt gtatggcgac cagggcgaac agcctctgcg cgaagacgcc
     8161 atccccaacc cgcgctcacc ttatgcagtt tctaagctgg cggcggaata ctacgtgcgc
     8221 acgattgggg accagtgggg catcgagacg gtctgcttgc gggtcttcaa tgcctacggc
     8281 ccgggtcagc atttgcctgc ttcccatcct cctgtaatcc cgtatttcct taaacaggcg
     8341 gtgcgcggag gtactttggt gttgcatggc gacggcaccc aaacgcgcga ctatgtttac
     8401 gttagcgatg tagtgcgctg catggtatct gcggcgactg cacccaatat caataaaatg
     8461 atcatcaatg tgggcagcgg cactgagacg agtgtgcgcg agctggtacg gttggtgagg
     8521 aaagttgtca ctgggggaaa gccggagtta atttttaacc ctcgcaccca cccgggtgtc
     8581 tcgcgcatgt gcgccgattt gaccctggcg cgtgaaaagc tgggcttcca gccacgcgtt
     8641 tcactggtcg atggactcag catgactctg gagcgcgatc tgcgttttaa gaaagagcct
     8701 actcggcccc tgcgctaatt tatgcccgac cccgagcaat tagaacgacc ttttttctcc
     8761 cgaagcacct tacaagtggc gcgggaattg cttggcatgc gcctggtacg gcaggaaagg
     8821 gacgtgcgtt tggtgggtgt gattagtgag gtggaagctt accgtggtga agaggatcaa
     8881 ggctgccatg cgcgcgctgg ccggactccc agaacgcgcg tgatgtacgg ccccccgggt
     8941 cacgcttatg tgtactttac ctatgggatg cactggatgc tcaactttgt aaccgaggct
     9001 gagggttatc cggcggcggt gctgattcgt gccatcctgc cagtggaagg gttggatgtg
     9061 atcgccgcgc ggcgggatgg gcaacccttc gcccattgga ccgatgggcc agctaagctg
     9121 tgccaagcgt tgggcatcga taggcggcat aacggcctgg acctgtgcgc tccacaatca
     9181 gagatttttg tccaggtcgg cgaatctatc cccgattcag ccgtaaccat tggccctcgg
     9241 gtagggttaa ataatgtgcc agaaccctgg aaaagtatcg catggcgttt tagaatacaa
     9301 tccgcagatt ttactggtgt ttattaatgc agagatattg gtttaaccat tagtgttaac
     9361 ccgcagtaaa actgtatttg gagcataata cttatatcct gcgtctgtaa tgattgaatg
     9421 tggaggaata gatgaaatta cttgaaggta aaaccgcgct ggtatttggt ctagccaatg
     9481 aacgttcgat cgcttggggg attaccaagg cttttcatga gcatggcgcc accttgggca
     9541 tcagctacgc aggcgaagtc ttggagcggc gtgtccgccc ccttgcagcg gagat
//
LOCUS       DBNGSX010000152        14503 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1742910, whole genome shotgun sequence.
ACCESSION   DBNGSX010000152 DBNGSX010000000
VERSION     DBNGSX010000152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14503)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14503)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14503
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1742910"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="AC16CB_04950"
     CDS             <1..250
                     /locus_tag="AC16CB_04950"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIR3341852.1"
                     /translation="YVQGVGFRYFVQENAVTLGLRGWVRNLWSGDVELVAEGDRQALD
                     KLIAALRRGPPTAHVSGVRTEWGDFTGEFRSFFVKSTI"
     gene            530..1798
                     /locus_tag="AC16CB_04955"
     CDS             530..1798
                     /locus_tag="AC16CB_04955"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3341853.1"
                     /translation="MARWGKLLIVLVLVLTMVPVLPALAQTGIVCDYYGVHGNGAKYC
                     ITLPSEGDWNGDLVIFAHGYVSVREPLEIPWDQLILPDGTPMPAIVNNLGYAFATTSY
                     SENGLAVVRGVIDILDLVEVFNDTVGRPNRVYLVGASEGGLVTTLAVEQHPGPFAGGM
                     AMCGPIGGFRGQVDYWGDFRVVFDYFFPGLLPGSPVNVPDEVMDNWESVYAPGVLVAI
                     QANPLNAQQLLSVTQAPIDPFDPSTIPQTVLGILWYNVFATNDGIEKLRGQPFGNVTV
                     ERIKRMTIYAEKWYMGSLDDQALNQDVQRFIADQSALNKIDKKYETSGKLKKPLVTLH
                     TTGDPIVPYWHAIKYGAKVDENGMGGAYSHIPVNRYGHCNFELAEILGGFGWLVLQTT
                     GQPLNMAGLSLDAASQARIFEFSQGVVSGE"
     gene            complement(1949..2650)
                     /gene="radC"
                     /locus_tag="AC16CB_04960"
     CDS             complement(1949..2650)
                     /gene="radC"
                     /locus_tag="AC16CB_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HIR3341854.1"
                     /translation="MTSQRPSYRITDLDEAERPRERLQRLGAQALSSAELLAILLRVG
                     VHGENAVQVGQRLMQTFGGLPGLHRASFDEVCAQRGIGEAKAAQVKAAIELGRRLAAE
                     APDERPAIHSPQDAADLVRYEMSALEQEELRVILLDNRNRMITVEAVYRGSVNSSQVR
                     VGELFKAAVRRNAAALIVVHNHPSGDPTPSPDDVAVTRAIVQAGKLLDIDVLDHLVIG
                     GGKFISLKERGLGFG"
     gene            2847..3359
                     /locus_tag="AC16CB_04965"
     CDS             2847..3359
                     /locus_tag="AC16CB_04965"
                     /inference="COORDINATES: protein motif:HMM:NF024183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR3341855.1"
                     /translation="MTFDPSAFSNVILIVTAWGGAFIAALWLSLVIWTYRDIRGRARD
                     PLGRILAVLVVAVLFLPGIVIYLILRPARTLEEEYQQSLEEEALLHSIEDASLCPGCG
                     RRIKENWMVCPGCHTKLKKNCHQCGKLMELGWNLCPYCGTPAPGMRREGLTMDDALRP
                     MPIEEEQASE"
     gene            complement(3442..4731)
                     /gene="eno"
                     /locus_tag="AC16CB_04970"
     CDS             complement(3442..4731)
                     /gene="eno"
                     /locus_tag="AC16CB_04970"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR3341856.1"
                     /translation="MDLIIEDVVGREILDSRGNPTVEVEVTLMDGSWGRAAVPSGAST
                     GVHEALELRDGDKSRYGGKGVLKAVEHVNNQIADLLLGWDASDQKGIDAALLELDGTP
                     NKANLGANAILGTSLALAKAAANALGLPLYRYIGGVYAHVLPVPMLNILNGGAHTSWQ
                     STDAQEFMVMPLGASSFAEGLRWGAEIYHALKTVLKGRGYTALVGDEGGYAPALKANA
                     EAVEVILEAIQKAGYKAGEQVAIALDPAASELYEEDTGLYNLRKEGKKLTGEQMVAFW
                     KSWVDQYPIVSIEDGLAQDDWESWQLMVQELGDRLQIVGDDLLVTNPDRVRRAIKEKA
                     ANALLVKLNQIGTLTETIEAVETCQRAGWRAVTSHRSGETEDSTIADLAVALNMGQIK
                     TGAPARSDRVAKYNQLLRIEAELGETAKYAGWSALNK"
     gene            4848..5534
                     /locus_tag="AC16CB_04975"
     CDS             4848..5534
                     /locus_tag="AC16CB_04975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3341857.1"
                     /translation="MSAETSPELTDPRDRLTYHFDYTPFRRVLTSTVQTLFHLIAIID
                     ASGVEHLPKEGPVVLAANHVTNFDVFPMQFSLPRPIFFMAKAELHRNPILDVALRQMG
                     SFPVERGARDEWALRHAQKVLEHRQMLGIFPEGKRNMGRGLRPAKTGAARLALSVNCP
                     IVPLAVEGTQRMFQRFPRRTLVHVTVGEPIFPQHGDTALSLTDQMMFSLAELLPPEQR
                     GVYAQHPTGF"
     gene            complement(5573..7222)
                     /locus_tag="AC16CB_04980"
     CDS             complement(5573..7222)
                     /locus_tag="AC16CB_04980"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR3341858.1"
                     /translation="MATKFIFFTGGVVSSVGKGVTAAAIGRLLRERGFNVAVQKLDPY
                     INVDPGTMSPYQHGEVFVLDDGAETDLDLGHYERFIDISLNSVCNVTTGQVYADVIGK
                     ERRGDFLGGTIQVIPHITNEIKRRISLVAKTTGAEIVLVEIGGTVGDIESLPFLEALR
                     QMRSDLGRENTVYIHVTWLPYIGATRELKTKPTQHSVRELRSIGISPDVIVARSDYPV
                     DEDLCEKIALFCDVERKAVFPMVTTDVLYEIPLLLERQHAGDYLLDRLGLKPRRQPDW
                     QEWEQLVQEVRKSKPVVNIALVGKYVELQDAYMSVREALKHAAISQGVEVEISWVHSA
                     ELEKGRSWEMVRNADGIIVPGGFGSRGIEGKIQAAHYARENKIPYLGLCLGMQLMVIE
                     FARHVLGKEEVNSTEFDRSTPYPAIDLMPDQKSISDMGGTMRLGLYPCQLQPGSIAAK
                     AYQKKVVQERHRHRFEFNNAYREILAKAGMRYSGISPDGRLVEIAELTNHPFMLGTQF
                     HPEFLSRPNRPHPLFVAFVKAVRERSGVQVGLEVDKLKIEG"
     gene            complement(7320..7670)
                     /locus_tag="AC16CB_04985"
     CDS             complement(7320..7670)
                     /locus_tag="AC16CB_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HIR3341859.1"
                     /translation="MVDRLREMQASSPEIEASAVVSVDGLIIASALPADAEEDRVSAM
                     SAAMLSLGERIANELGRGTLDQVYIRGNSGYVILSAIGEEAVLTALAREGAKLGLVFL
                     EMRRAAEDLLRLVE"
     gene            7935..8570
                     /locus_tag="AC16CB_04990"
     CDS             7935..8570
                     /locus_tag="AC16CB_04990"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009785910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR3341860.1"
                     /translation="MRIYDISLPILPGMPVWPGDPQVIMDRVGNMDEGADANVTHLSM
                     SVHTGTHVDAPYHFMGGEAATVDLLSLKLLTGRAYVLHLPDIDLITADVLEQADIPPR
                     TRRLLFRTRNSDHWGNPSHEFHTNFVAINSDGAQHLVDRGVKLVGVDYLSVAPYGDST
                     PTHRILLSAGMLIVEGLDLSGVSQGRYTLHCLPLKLVGCDGAPARAILIGV"
     gene            8585..9022
                     /locus_tag="AC16CB_04995"
     CDS             8585..9022
                     /locus_tag="AC16CB_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HIR3341861.1"
                     /translation="MKLSSTAFAEGDAIPRKYTCDGDNVSPALSWQGAPQATKSLVLI
                     MDDPDAPSGTFVHWILFDIPPAFSQLREGVRGVGMPGANSYRKVGYNGPCPPPGPAHR
                     YFFKVYALDQLLRLRPGVIKAELEGAMQGHILDSGQLMGRYGR"
     gene            complement(9063..9507)
                     /gene="rnpB"
                     /locus_tag="AC16CB_05000"
     ncRNA           complement(9063..9507)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="AC16CB_05000"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            9864..11371
                     /locus_tag="AC16CB_05005"
     rRNA            9864..11371
                     /locus_tag="AC16CB_05005"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            11514..11587
                     /locus_tag="AC16CB_05010"
     tRNA            11514..11587
                     /locus_tag="AC16CB_05010"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11548..11550,aa:Ile,seq:gat)
     gene            11608..11680
                     /locus_tag="AC16CB_05015"
     tRNA            11608..11680
                     /locus_tag="AC16CB_05015"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11641..11643,aa:Ala,seq:tgc)
     gene            11873..>14503
                     /locus_tag="AC16CB_05020"
     rRNA            11873..>14503
                     /locus_tag="AC16CB_05020"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         3362 a         3717 c         4120 g         3304 t
ORIGIN      
        1 ctatgtgcag ggggtgggct ttcgctattt tgttcaggaa aatgcggtta ccctggggct
       61 gcgcggttgg gtgcgcaatc tttggagcgg ggatgtcgaa ttagtggcag aaggagaccg
      121 ccaggcgctg gataaactga tcgccgcctt gcgccgcggg ccgcccaccg cccacgtgag
      181 cggagtacgc acagagtggg gggattttac aggggagttc aggagttttt tcgttaaatc
      241 gactatctga cggtcaaacc tgccaacggc ttccacactc caaagattgc cttcttgtga
      301 aattctcgat ttaccctttt cgagggttga attacataaa aaaattgcta taatgattta
      361 tagcatagca taattaactg ggtatttttt tcgcgacagg gtttgtgctt cgttcttata
      421 gcactgacca acggtagtta tgttattgct gggcttgaat tttttattag tagggcggtg
      481 attatcagat attcaaatag tatgactgat ttgtaaaagg aggtctatca tggctcggtg
      541 gggaaaactg ttaatagtcc ttgtcctggt ccttactatg gtccccgtct tgcctgcatt
      601 ggcgcaaaca ggtattgtat gtgactacta tggagtgcat ggcaatggag ccaaatattg
      661 catcacgttg ccctctgaag gtgattggaa tggcgatcta gtcatttttg cgcacgggta
      721 tgtttctgtc agagagccat tggagatccc atgggaccag ctgatcttgc cggatggcac
      781 tccaatgcct gccattgtaa acaatttagg atatgcattt gccaccacga gctacagcga
      841 gaatgggctg gcggtcgtgc ggggagtaat tgacatcctc gatctggtgg aggtgttcaa
      901 cgatactgtc ggccgcccca accgggtcta cctggtgggg gcttcggaag gtgggttggt
      961 cacgacgctg gctgttgaac agcatccggg tccatttgcc ggggggatgg ccatgtgcgg
     1021 gccaattggg ggcttccgcg gccaggttga ctactggggt gatttccggg tagtgttcga
     1081 ctattttttc ccaggcctat tacctggcag tccagtcaat gtccctgacg aagtgatgga
     1141 caattgggaa agtgtatatg cccccggtgt tttggtggca atacaagcta atccattgaa
     1201 tgctcagcaa ctcttaagtg tcacccaggc accaattgat ccctttgatc ccagcacaat
     1261 tccgcagacc gtgctgggta tcctgtggta caacgtgttc gccacaaatg acgggatcga
     1321 gaagttgcgc ggccaaccct tcggaaacgt caccgtggag cgaataaaac ggatgacaat
     1381 atatgcagaa aagtggtaca tgggctcact cgacgatcaa gccctcaacc aggatgtaca
     1441 gcgcttcatt gccgatcaat ctgccttaaa caagatagat aaaaaatacg agacctccgg
     1501 caaactgaaa aagccattgg tgaccctcca taccacgggc gacccgatcg tgccctactg
     1561 gcatgctata aaatacggtg cgaaagtgga cgaaaatggc atgggtggag catattctca
     1621 tataccagtt aatcggtatg gtcattgcaa ttttgagctg gctgaaatcc tgggtggatt
     1681 tggctggctt gtgctccaaa ccacagggca accgctcaat atggctgggc tttctctgga
     1741 tgcagcctca caggcgagaa tctttgaatt cagccaggga gtcgtctctg gggagtgaac
     1801 ttctctaaaa acaatatata gcatgagtct aaagagcact tgcgcacgat tttgaaaaac
     1861 gcaggtgctc tttcgtatta ctttggtgta tttgattacc agctgcaaat tgggatgcga
     1921 taatgggtaa gtgtcggtag caataaacct aaccgaatcc caagcctctt tctttgagcg
     1981 agataaactt gcccccgcca atcaccaagt gatctaaaac atcgatatcc aggagcttgc
     2041 cagcctggac gatggcgcgc gtcaccgcaa cgtcatctgg gctgggggtg gggtcgccgc
     2101 tggggtgatt gtgcaccacg atcagcgcag cggcgttgcg ccggacagcg gctttgaaca
     2161 gctcgcccac ccgcacctgg gaggaattta ccgagccgcg atagaccgct tcgacggtga
     2221 tcatccggtt gcgattgtcc agcagaatca cgcgcaactc ctcctgctcc aatgcactca
     2281 tctcgtagcg aactaggtcg gcggcatcct gcggactgtg tatcgctggg cgctcatccg
     2341 gggcttcagc agccaggcgg cggcccagct cgatggctgc ttttacctgg gcagccttgg
     2401 cctcgccaat tccgcgctgg gcgcacacct cgtcaaacga agcgcgatgc agccccggca
     2461 aaccgccaaa ggtctgcatc aagcgctgcc cgacctgcac agcgttctcg ccgtgcactc
     2521 ccaccctgag caggatagcc aatagctcag cggatgagag cgcctgggca cccagccttt
     2581 gtagtcgctc gcgcggccgt tcagcctcgt ccaggtcggt gatgcggtag gacgggcgtt
     2641 ggctagtcat ggtgaatcaa ggtgggttga atgttgcgga agggggtggg gttgtccatt
     2701 ggcaatctcc tgcaagtttg gcagatgaag ttatcttgga cagtcgagct acctggtaga
     2761 agtattcccc ggcagcacta tataatttta ctctggaagt catctgatct ccaataatgc
     2821 tataatgtac tcccagggag cctgctatga cctttgatcc gtctgccttc agcaatgtta
     2881 ttctcatcgt gaccgcctgg ggaggggcgt tcatcgctgc cctttggctg agcctggtaa
     2941 tctggaccta ccgcgatatc cgcgggcggg cgcgcgaccc tctcgggcgc atcctggcgg
     3001 tgctggtggt ggcggtgttg tttttacccg ggattgtcat ctacctgatc ttacgcccgg
     3061 cgcgcaccct ggaagaggaa taccagcagt cccttgaaga ggaagccttg ctgcactcga
     3121 tcgaagatgc cagcctgtgt ccgggttgcg gcaggcgcat caaggaaaac tggatggttt
     3181 gcccaggttg ccatacgaag ctgaagaaaa attgccacca atgcggcaag ctgatggagc
     3241 taggatggaa cctgtgccct tactgtggta cacctgcccc cggaatgcgc cgcgaaggtc
     3301 tgaccatgga tgatgccctg agacctatgc caattgaaga agagcaagct tccgagtaaa
     3361 tgtagttatt aaaacgaagg acaccaagga aacataaagg gtgttttaga ttccttggtg
     3421 ccctttttgt tttatttgtt cttatttatt taacgcactc caccctgcat attttgccgt
     3481 ctcacctagc tcggcttcga tgcgcagcaa ctggttgtat ttggcaacac gatcggagcg
     3541 agctggagca ccggtcttga tttgccccat attgagcgcc accgctaggt cggcgatggt
     3601 ggaatcctcg gtttcgcctg atcggtgtga ggtgactgct cgccagccgg cgcgctggca
     3661 ggtctccact gcttcaatgg tctcagtgag cgtgccgatc tggttgagct taaccaacaa
     3721 ggcattggca gctttctcct tgatggcacg acgcacccga tcggggttcg tgaccagcag
     3781 gtcatccccc acgatttgaa gacggtcccc gagctcctgg accattaact gccagctttc
     3841 ccagtcatcc tgagccagcc catcttcaat cgagacgatt ggatactggt cgacccagga
     3901 tttccagaag gcgaccatct gctcgccggt cagcttttta ccctcttttc gcaggttgta
     3961 caagccggta tcttcctcgt aaagctccga tgctgctgga tcgagagcaa ttgctacctg
     4021 ttccccagcc ttgtagccag ctttctgaat agcctcgagt ataacctcga ccgcctcggc
     4081 gttagccttc aatgcaggcg cataaccgcc ttcatcgccg accagggcgg tgtagccgcg
     4141 tcctttgaga acggttttga gggcgtgata gatctctgct ccccagcgta gaccttcagc
     4201 gaagctcgat gcccccagag gcattaccat gaactcctgg gcgtcggtgg attgccagct
     4261 tgtatgggcg cctccattaa ggatgtttag cataggtaca ggaagcacat gggcatatac
     4321 gccaccaatg tagcggtaga gcggcaagcc aagagcgtta gccgcagcct tggcaagcgc
     4381 aagactggtg ccaagaatgg catttgcgcc caaattggcc ttattgggtg tcccatccag
     4441 ctcgagtaag gcagcgtcaa tgcctttctg gtcactggcg tcccagccca gtagcaggtc
     4501 tgcaatttga ttgttgacgt gctcaacagc tttcaatacg cctttccccc catagcgaga
     4561 tttgtcgcca tcgcgcaatt ccagcgcttc gtggacgccg gtggatgccc ccgaaggcac
     4621 tgctgcccgc ccccagcttc catccatcag ggtcacctcc acctcaacag tggggttgcc
     4681 gcgcgaatcg agaatctcac gaccaacgac atcttcgatg atcaaatcca tagttcacct
     4741 cgttgatatt gaaaaagtga tgcagttgaa tgtttatcct tcaaccgcgt taatccgata
     4801 tccgttaaaa tattgattaa gccgaaatgc aagtataatg caattcaatg tctgcagaga
     4861 cctccccaga acttacagat ccccgcgatc gccttacgta ccattttgat tatacgccct
     4921 ttcgccgcgt attgacatct accgtccaga ccctattcca tctcatagcg attatcgatg
     4981 cgagtggtgt ggaacacttg cccaaagaag ggccggtggt gttggctgcc aaccatgtca
     5041 ccaacttcga tgtcttccca atgcaattta gcttgccacg accgatcttt tttatggcta
     5101 aggccgaatt gcatcgcaat cctatcttgg acgttgccct gcgccagatg ggtagcttcc
     5161 cagtggagcg cggcgcccgg gatgaatggg cgctacgcca tgcccaaaaa gtgctggaac
     5221 ataggcagat gttgggcatc ttcccagaag gaaagcgcaa tatgggtcgg ggcttgagac
     5281 cagccaagac tggtgctgct cgattggctt tatccgtgaa ctgcccgatc gtgccgctgg
     5341 cagttgaagg aactcagcgc atgtttcaac gctttccgcg ccgtacgctt gtgcacgtca
     5401 cggtaggtga gcccattttc ccacagcatg gcgatacagc cctgtcgctc accgatcaga
     5461 tgatgttctc actggctgag ttgttgccac cagagcagcg tggcgtgtac gcgcaacatc
     5521 ccactgggtt ttaataccaa caaacccgag ctattcccca ggtttaattt ttctagcctt
     5581 caatttttaa tttgtcgact tccaagccta cctgaacccc agaccgctct cgcacggctt
     5641 tcacaaaggc gacgaacagg ggatgtggtc gattgggacg tgagaggaat tctggatgaa
     5701 actgcgtccc aagcataaag ggatggttgg tcaattcggc gatctccacc agacgaccgt
     5761 cgggggagat gccggaatag cgcattcccg ctttagccaa tatctcccga taagcattgt
     5821 taaactcgaa gcgatggcga tgacgttcct ggacgacctt cttctggtag gctttggcgg
     5881 cgatgcttcc gggctgcaat tggcacgggt acaatcccaa gcgcatggtg cctcccatat
     5941 cggatatact tttttgatcg ggcattaaat cgattgccgg gtagggtgtg gaacgatcga
     6001 actcggtaga attgacctct tctttgccca aaacgtggcg agcaaactca ataaccatca
     6061 gctgcatgcc caggcaaagt cccaggtagg gaattttgtt ctcgcgggcg tagtgtgctg
     6121 cctgaatttt gccctcaatc ccgcggctcc caaacccgcc cgggacgata atgccatccg
     6181 cgttacggac catttcccaa ctgcggcctt tctccagttc ggcagagtgc acccaagata
     6241 tctcaacttc gaccccctgt gagatggcag catgttttaa cgcttctcgc acactcatgt
     6301 aggcatcttg taattcgaca tatttcccga ccaaggcgat attcacaaca ggtttggact
     6361 tgcgcacttc ttgtaccaac tgctcccatt cctgccagtc tggctggcgc cgcggcttaa
     6421 gacctagcct gtctaaaagg taatcaccag catgctggcg ttcgagcagc agagggattt
     6481 catataatac atctgttgtc accattggga agactgcctt gcgttcgaca tcgcagaaga
     6541 gggcgatttt ctcgcacagg tcctcatcga cgggataatc cgaccgggcg actatgacgt
     6601 cgggtgagat accaatggag cgcagttcac gcaccgaatg ctgcgttggc ttggttttaa
     6661 gttcacgggt cgccccgatg taaggcagcc aggtcacgtg gatgtagacg gtattctccc
     6721 gccctaaatc gctacgcatc tggcgcaagg cttccagaaa gggtaaggac tcgatatcac
     6781 ccaccgtccc gccaatctcg accagcacaa tctcggcgcc cgtagttttg gcaaccaagc
     6841 tgatgcggcg tttgatctcg ttggtgatat gcgggatgac ctggatcgtg ccacccaaaa
     6901 aatcccctcg tcgctcttta ccaatcacat cggcatagac ctgccctgtt gtaacgttac
     6961 atacactgtt caggctgata tcaatgaatc gctcataatg gcccaggtcc aggtcagtct
     7021 cggcaccgtc atcaaggacg aacacctcac catgctggta agggctcatc gtgcccgggt
     7081 ccacgttgat gtatgggtcg agcttttgca cagcgacgtt aaagccgcgc tctctaagga
     7141 gtcgcccaat tgctgcagcg gtgacacctt tgcctactga gctaactact ccaccggtaa
     7201 agaatatgaa cttggttgcc attcgtgcct ccattggtcg gtgttaaagg aagtggggag
     7261 gtccgtttag cggggacctc cccacgaggg tgtcaatata agggaacctt gctggtaaat
     7321 cattctacca acctcaatag atcttctgct gctcgccgca tttcgagaaa aaccaatcct
     7381 agcttggcac cttcgcgcgc caatgcggtt agcacagcct cctcaccgat ggcggacaga
     7441 attacgtaac cgctgttgcc ccggatatat acctggtcca aggtacccct gcccagctcg
     7501 ttggcaatgc gctcaccgag gctgagcatg gcagcagaca tcgctgagac tcgatcttct
     7561 tctgcatcgg ctggtagcgc agaggctatg atcagcccat ctacagaaac tacagcggag
     7621 gcttcgatct caggagatga cgcctgcatt tcgcgaaggc ggtcgaccat caattctgtt
     7681 cgcgagcggg tcaaggcgat gtccactcct catgaggatt gattgaaccc gccatgagtc
     7741 taccataact gatagactaa tgcaaaagca ggttcttcgt tgtctgagat attctggctg
     7801 gcgtcgctgc aaaggttgga ggccaataca tccttaattt ggcggcgcat gacgagaatt
     7861 ctcacacaga ccacgccatt aagttgcttt ttaaaatgcc ttgggctata ctttttaata
     7921 atcacggagg gagtatgcgc atatatgata tttccttgcc catattgcct ggaatgcccg
     7981 tgtggcccgg agacccgcag gttattatgg atcgggtagg gaacatggac gaaggcgcgg
     8041 atgccaatgt gacacatcta agcatgagtg tccatacagg cacgcacgtt gacgctccct
     8101 accattttat ggggggggag gcggcaaccg ttgacctatt atcgctcaag ctgttgactg
     8161 gcagagccta tgtgctgcat ttgccagaca tagatctcat caccgcagat gtgctggagc
     8221 aggcggatat ccctccacgc acgcgtcgct tgctctttag aacgcgtaac tctgatcatt
     8281 gggggaatcc ttcgcatgag tttcatacca attttgtggc catcaactcg gatggcgccc
     8341 agcatctggt ggatcgaggc gttaagctag tgggtgtgga ctatttgtca gtggctcctt
     8401 atggcgatag tacaccaaca caccggattt tgctgagcgc aggcatgctg attgtggagg
     8461 ggctggactt gtctggcgtc tcgcaaggcc gctatacgct acattgtcta cccctcaaat
     8521 tggtcggctg cgacggggcg ccggctcggg cgattctaat tggcgtttag caggaggtaa
     8581 gccaatgaaa ttaagcagta cggctttcgc agaaggagat gccatcccac gcaagtatac
     8641 ctgcgacggc gataatgttt ctccagcact ctcctggcag ggagccccgc aggctacaaa
     8701 gagcctggtg ttgatcatgg atgacccaga tgccccatcc gggacctttg ttcactggat
     8761 tctattcgac atccctcccg cattcagtca gctgcgcgaa ggcgtgcggg gtgtgggcat
     8821 gccaggggca aacagctatc gcaaagtggg ctacaatggt ccctgcccgc cgccaggccc
     8881 agcgcaccgc tatttcttta aagtatatgc actggatcaa ttgctcaggt tgcgtccggg
     8941 ggtcatcaaa gccgagctgg aaggcgccat gcaggggcat atcctagact caggccagtt
     9001 aatgggtcgc tacgggcggt aattctagcg tttgcagagc gttagttttc aggaataaga
     9061 aaatcccagg ccagtctata agccgcattc tgtagggcag gaacctgccc tggtgatcat
     9121 ctctctgggc tgcgcgtctc cgcgcagctc ttgcagccta cccgggactt tggaccggag
     9181 tcctggagac gagcaatctc cttagattgc tgattataga tatccaattg ctgattggtt
     9241 cttcaaccaa caatctgcaa tcatcattca acaatcctct tgtccctgct tggccttgct
     9301 cctggcgggg gttgcctggc cgaccgcatt gctgcgggcg ccggtggtct cttacaccac
     9361 cttttcaccc ttgccgaacc ggcggtttgt ttctgtggcc cgatccaaca ggttaccccg
     9421 ttccggggat tacccggcgc cgtgctctat ggagtgcaga ctttcctcga ctccggaaaa
     9481 cggagccgcg atcacccgac caacctgggt gagaatcatg ataacatatc ttgacagaat
     9541 tgtcaggtga agtataatag ggattgcaaa cgacaatcga ggtctcgccc gaacagggaa
     9601 gatcgattac aactgagcag gacgcctctg cgccagagac cgaagcgaaa ccgctttagg
     9661 tctgacgggc cgatgtcaga gagtatgggc atcggcagtt gtgtctgtgt gcaaatcgac
     9721 ttagagcctt gacaggcgta agtgaggctg gtacaatcca gcccgcttga aagaccagga
     9781 ccttaacaac tgaagagtga tagaaagtca gccttgtctt ttccaggact tccgtaacca
     9841 taacgcacta agtgcgtaaa agttttgcgg agagtttgat cctggctcag gatgaacgct
     9901 ggcggcgtgc ttaatacatg caagtcgaac ggggactttg tattcgtaca tagtcctagt
     9961 ggcgaacggg tgagtaacgc gttggtgacc tgccccgaag agagggacaa cagctcgaaa
    10021 gggctgctaa tacctcatgt ggtcgtaagg attagatgcc ttgcgtctaa atggagcaat
    10081 tcgcttcggg aggggcctgc gtcccatcag cttgttggta gggtaacggc ctaccaaggc
    10141 aacgacgggt agggggcctg agagggtggc cccccacact ggcactgaaa cacgggccag
    10201 acacctacgg gtggcagcag tagggaatat tgcacaatgg gcgaaagcct gatgcagcaa
    10261 cgccgcgtgc acgatgaagg ccttcgggtc gtaaagtgct tttcggggag atgagagagg
    10321 acagtatccc cggaataagg atcggctaac tacgtgccag cagccgcggt aaaacgtagg
    10381 atccgagcgt tatccgaatt tactgggcgt aaagcgcgtg taggcggttc ggtaagttgg
    10441 atgtgaaatc tcccggctca actgggagag gtcgttcaat actgtcggac tagaggatgg
    10501 tagagggagg tggaattccc ggtgtagtgg tgaaatgcgt agatatcggg aggaacacct
    10561 gtggcgaaag cggcctcctg gaccattcct gacgctcaga cgcgaaagct agggtagcaa
    10621 acgggattag aaaccccggt agtcctagcc gtaaacgatg tggacttggc gttggtgggg
    10681 taaaagccat cagtgccgaa gctaacgcga taagtccacc gcctggggac tacggccgca
    10741 aggttaaaac tcaaaggaat tgacgggggc ccgcacaagc agcggagcgt gtggtttaat
    10801 tcgatgctac gcgaagaacc ttacccgggt ttgacataca ggtggtaggg atctgaaaag
    10861 tgaccgaccc ttcggggagc ctgtacaggt gctgcatggc tgtcgtcagc tcgtgccgtg
    10921 aggtgtccgg ttaagtccgg taacgagcgc aacccacgcc gtatgttaca agtgtcatac
    10981 gggactgccg gtataaagcc ggaggaaggt gtggatgacg tcaagtcagc atggccttta
    11041 tatccggggc tacacacacg ctacaatggc cagtacaatg ggttgcaaag ccgcgaggta
    11101 gagccaatcc ccaaagctgg tctcagttca gattgtaggc tgcaactcgc ctacatgaag
    11161 tcggagttgc tagtaaccgc aggacagcta tactgcggtg aatacgttcc cgggccttgt
    11221 acacaccgcc cgtcacgtca tgggagctgg taacacctga agccggtatc ctaacccgta
    11281 agggagggag ctgtcgaagg tgggactggt gactgggacg aagtcgtaac aaggtaggag
    11341 taccggaagg tgcgcctgga tcacctcctt tctaagagtt ctgagcaact ttctggcatc
    11401 ctcgtgatgc ggattgaagc tctactcact aacaaatcgt ctcatcgcgg tgatgagacg
    11461 tctggaagca agtcgctttc tatcactcct cagttgctaa ggcctgtgtt ggcgggcctg
    11521 tagctcaggt ggttagagca ctgtgctgat aacgcagggg tcactggttc gaatccagtc
    11581 aggcccactt cccggtggtt taaccctggg gatgtagctc agttgggaga gcgccgcctt
    11641 tgcaaggcgg atgtcagggg ttcgagtccc ctcatctcca cctgactgcg agatcgaagt
    11701 tagtcaaaag gacagagctt gatcgaattg ttgaatttct aatcaattca gggcaaaatc
    11761 gtaagggtaa agcgacccgg cgatttgccg ggtcgctttg tctcgaaaat agtaccttac
    11821 caactgaata ggcgcattaa tgaagaaaag ttttgatttc tccgcatcac gaagttaagc
    11881 tactaagagc ttacggtgga tgccttggcg ccaagtgccg atgaaggacg tgggacactg
    11941 cgataagcct cgggtagccg tgtacaggcg ttgatccggg gatctccgaa tggggaaacc
    12001 cgccgctgca aaccagcggc atctcctagt tgaacacata gaccaggaga agggcacctg
    12061 gggaactgaa acatctaagt acccagagga aaagagatta ttcccctagt agtggcgagc
    12121 gaacggggat cagcccaaac catcccggct tgctgggatg gggttgtagg gctcgacaca
    12181 tgggagttac aaatcgatct gttagcagaa aggtatggga aagcctacca aagagggtga
    12241 cagtcccgta tgcgaaaata gatcgactcc cgtcgagtac ctgagtacca ccgggcacgc
    12301 taatccggtg ggaagctggg gagtccactc tccaaggcta aatacacttg gcgaccgata
    12361 gtgaacaagt accgtgaggg aaaggtgaaa agtaccccgg tgaggggagt gaaatagaac
    12421 ctgaaaccgt aagcttacaa gcagtcggag ggctaatggt gggtctgtgc gccggcgaaa
    12481 gccggaagcg cgccactatg cctgacggcg tgccttttgg agaatgagcc tgcgagttaa
    12541 tctctgtggc aaggttaagg gagtgacacc cgaagccgta gcgaaagcga gtctgaatag
    12601 ggcgtacagt cgcagggatt agacacgaaa ccgggtgagc tacccatggc cagggtgaag
    12661 cgagtctaac cgctcgtgga ggcccgaacc cactaacgtt gcaaagttag gggatgagct
    12721 gtgggtaggg gtgatatgcc aaacgaaccc ggagatagct tgttctcccc gaaatagctt
    12781 tagggctagc gtcatgtgtt cagtaccgga ggtagagcac tggatgggct agggggctta
    12841 ctgcttaccg aacccaacca aactccgaat gccggtactc catagcatgg cagtcggact
    12901 acgggagatg agtttcgtag tcgaaaggga aacagcccag accatcggtt aaggtcctta
    12961 aatctatgct aagtgggaaa cgatgtggga ctacacggac aaccaggagg ttggcttaga
    13021 agcagccacc ctttaaagag tgcgtaatag ctcactggtc aagtggtcct gcgcggaaaa
    13081 tttaacgggg ctaagcatag taccgaaacc atgggtgtgc ggtgatcttc gggtcaccgc
    13141 gtacggtagg ggagcgttct gccagcggtg aaggtcgacc gtaaggacgg ctggagcggg
    13201 cagaagtgag aatgcaggca tgagtagcgt aaaacacgtg agaaccgtgt tcgccgtaag
    13261 tctaaggttt ccgacggcag gtcaatccgc gtcgggttag tcgggcctta gccgaggccg
    13321 agaggcgtag gcgatggata tccggtcaac attccggaac cacctaggag gagcgatgag
    13381 gggacgcagt gaggtaggcc agcccacgat tggtagtgtg ggtgtaagcg tgtaggcgtt
    13441 gaagtctgtt ggtaaatccg cagaccgagc tgaggcgcga atgcgagccc ctgaggcaga
    13501 agtggttaag cctcgctgcc gagaaaagcc tctaagtgtt gaatcctagg tgtccgtacc
    13561 gcaaaccgac actggtagac gggtagagta taccaaggcg atcgggagaa ccttcgttaa
    13621 ggaactaggc aaattagccc cgtaacttcg ggagaagggg tgccctactg cgtgaaattt
    13681 atacagatgg agcgcggtgg ggtcgcagtg aaatggctct agtgactggt taacaaaacc
    13741 acaggtctct gcaaagccgt aaggcgatgt ataggggctg acacctgccc agtgccggaa
    13801 ggttaagggg aggggttagc gcaagcaaag ctctgaaccg aagccccggt gaacggcggc
    13861 cgtaactata acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg
    13921 aacggtgtaa caactggagc tctgtctcaa cgaaggaccc ggtgaaattg aaatacgtgt
    13981 gaagatgcgc gttacccgca gcaggacaaa aagaccccgt ggagctttac tgtagcttgg
    14041 cattgagttt gggcatggtc tgtgtaggat aggtgggagg ctgtgaagct ggggcgttag
    14101 cctcggtgga gccaaccgtg aaataccacc ctgattatgt ctagacccta accccacgcc
    14161 gttaaccggg tgggggaccg tgccaggtgg gcagtttgac tggggcggtc gcctcctaaa
    14221 aagtaacgga ggcgcccaaa ggttccctca ggctgaatgg aaaccagccg tagagtgtaa
    14281 aggcacaagg gagcttgact gcaagaccaa cgcgtcgagc agagacgaaa gtcgggctta
    14341 gtgatcccac ggtaccgagt ggaagggccg tggctcaccg gataaaagct accccgggga
    14401 taacaggctg gtgaggtcca agagttcaca tcgacgacct cgctcggcac ctcgatgtcg
    14461 gctcatcgca tcctggggct ggataaggtc ccaagggtcg ggc
//
LOCUS       DBNGSX010000153         2417 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_219211, whole genome shotgun sequence.
ACCESSION   DBNGSX010000153 DBNGSX010000000
VERSION     DBNGSX010000153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_219211"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="AC16CB_05025"
     CDS             complement(<1..959)
                     /locus_tag="AC16CB_05025"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HIR3341862.1"
                     /translation="MNNILEIKTPAQSQASALKCDYGLESLGLMNLNRAYWNLPTEAL
                     YEEAVFRNEGKITRQGPIVVNTGIHTARAANDKMIVRESSSENNIWWGQYNRPYNPEN
                     FNDLFNRVQGYLQGRDIFIQDCYAGADPNYRIPIRIITEYAWHSIFARNMFLIPKTTE
                     DYYRHVPEFTVLAVPSFKSFPQIDGTATNTVITINFEQNLCIIGNSGYAGEIKKSIFT
                     MMNYLLPLQGVMTMHCSANVGSKGDVALFFGLSGTGKTTLSADPRRGLIGDDEHGWSD
                     DGVFNFEGGCYAKVIALSPNAEPQIYACTRKFGTILENVVFDPV"
     gene            complement(956..2188)
                     /locus_tag="AC16CB_05030"
     CDS             complement(956..2188)
                     /locus_tag="AC16CB_05030"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR3341863.1"
                     /translation="MSISQLARTIAESPTLALNEEARILRERGEPVINMSIGEPKNKA
                     PITAILSSAAKLTSGDVKYTPPDGTPSLKKAIIRYMEENYDRMVAPENVVISAGAKQS
                     LYNVLFSILNPQDEVIILAPYWVSYPEMVKMCYGVPVFVTPEDGSFHPRMEEIERAVS
                     SYTKAIIVNSPNNPSGCVFSKEFIAQIVEFCERKKIYLIMDDIYHKLVFDGIKAAPAY
                     HFTKQDIESTMVIVVNGVSKLYGMTGFRIGWVVTSRKLAEVITNVQAQTTSCTSVVLQ
                     AAAEGALTGMQSIVENLRLTFENNRDVIMQELSSFNGIRVIKPNGTFYILPDFRAYCP
                     SSLAKNSVELSKFLLKKALVVTIPGKEFGMEGYLRLSFSGSVKDITTGIERIKWALDP
                     QAPNEIYIGDRKLVRDWL"
BASE COUNT          605 a          559 c          599 g          654 t
ORIGIN      
        1 acaggatcaa aaaccacatt ttccaatatt gtgccgaatt tacgcgtgca ggcataaatt
       61 tgtggctctg catttggcga aagggcgatt actttggcgt agcacccacc ctcaaagttg
      121 aaaacaccgt cgtcgctcca tccatgttcg tcatcaccga ttaatccacg tcggggatca
      181 gcggagagag tggtcttgcc ggtaccagac aagccaaaaa acagggcgac gtcgcctttg
      241 gatcccacat tggccgagca atgcattgtc atgacgccct gcaacggcag taagtagttc
      301 atcatggtga agatggattt cttgatttca cctgcataac cggaatttcc gattatgcat
      361 aagttttgct cgaagtttat cgtaatgacc gtgtttgtgg cggttccatc gatttgcgga
      421 aaagatttga aagatggcac agcgagtacg gtaaactccg gcacatgtcg gtaatagtcc
      481 tctgtggtct ttgggatcag gaacatgttg cgggcgaata tgctgtgcca agcgtactct
      541 gtaatgatgc gaatgggaat gcggtaattg ggatcagcac ccgcgtagca atcctggata
      601 aaaatgtctc gaccttgtaa gtatccctgg acgcgattaa ataagtcgtt aaaattctct
      661 gggttataag ggcggttgta ttggccccac cagatattgt tctcagagct gctctcgcgc
      721 acgatcatct tgtcattggc tgcgcgcgcc gtgtgaatgc cagtgttaac gacaattggc
      781 ccttggcggg tgatttttcc ctcgttgcgg aatactgctt cttcatatag cgcttctgtt
      841 ggcagattcc agtatgcccg attgagattc atcaacccca ggctctctag gccataatca
      901 cacttgagtg ctgacgcttg tgattgggca ggtgttttga tctccaagat gttgttcata
      961 gccaatctct caccagcttg cgatcaccga tgtagatctc gttgggcgct tgcggatcta
     1021 atgcccactt tatccgctcg ataccagtag taatatcctt gacactaccg gaaaaactca
     1081 agcgaaggta gccttccatt ccaaattctt ttccgggaat ggtcacaacc agggctttct
     1141 tgagtaagaa cttagacagc tctacagaat tcttggcgag actagagggg caatatgctc
     1201 ggaaatctgg caggatgtaa aaagtaccat tcggcttgat gacgcgtatc ccattaaaag
     1261 aggacaactc ttgcataatc acatcgcgat tattttcaaa agttaggcgc aagttctcga
     1321 cgatgctctg catgcccgta agcgcgcctt cggcagccgc ctgaaggaca accgatgtac
     1381 atgaggtggt ctgtgcctgc acgttggtga tgacttcggc tagtttgcgg ctggtaacta
     1441 cccaaccgat acggaaaccg gtcatcccgt agagtttcga gaccccgtta accacgatca
     1501 ccatggtgga ttcaatgtct tgcttggtga aatggtaagc aggagcagct ttgatgccat
     1561 caaagacaag cttatggtag atgtcgtcca tgatgagata tattttcttt cgctcgcaaa
     1621 attcgacgat ctgagcaatg aactccttgg aaaacacaca cccagaagga ttattggggc
     1681 tattaactat gattgcttta gtatatgagc taacagcgcg ctcaatctcc tccatacgcg
     1741 ggtgaaaact accgtcctcg ggcgtgacga agaccggcac gccatagcac atcttgacca
     1801 tctcgggata gctgacccaa tagggggcca ggataattac ttcatcctga ggatttaaga
     1861 tactaaagag aacattgtac agcgattgtt tagcgccagc cgagatgacg acattctccg
     1921 gtgcaaccat gcggtcataa ttttcttcca tatagcggat gatggctttt tttagggagg
     1981 gtgtcccatc gggaggtgtg tatttgacat cgcccgaggt cagtttggct gctgagctca
     2041 ggatggcggt tattggggct ttatttttcg gttcgccgat gctcatatta atgacaggct
     2101 ccccgcgctc ccttaagatt ctcgcctcct cattgagcgc cagcgtgggc gactcggcga
     2161 ttgtccgcgc taattggctg attgacatgg gcacttttat ctccaaaatg agattttatc
     2221 gagagaaatc gatactccag ggtgagaaat atgaattttt cgataaggca attaattatg
     2281 catcaatgta gagtgtatct ctattcaaat taggcgtcaa tgtgacatta atcaagtacc
     2341 gcgacagggt aattgcatat gcccgcaagc ccgtaataag agtgtttggg gtggactttg
     2401 gctctttaac caaaatc
//
LOCUS       DBNGSX010000154         3297 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1535546, whole genome shotgun sequence.
ACCESSION   DBNGSX010000154 DBNGSX010000000
VERSION     DBNGSX010000154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1535546"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="AC16CB_05035"
     CDS             complement(<1..621)
                     /locus_tag="AC16CB_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR3341864.1"
                     /translation="MSKKLFTILSALLLVTLLLGACGPKPTEAPQQPVATEEPAAPEV
                     PAAPEETAAPEEPMEEGFTIGISNPFISSEYRTQMIAELIEVNQEYMDQGITTELVIE
                     SADTDVAGQIQQLQNLIAKDVDAILVNPGDVSGLNATLEEAVAKGIIVVSVDQELNVP
                     GVYNVGIDQKAWAMTSARWLADKLGGEGDIVQIEGFPGHPANVARME"
     gene            complement(1068..2264)
                     /locus_tag="AC16CB_05040"
     CDS             complement(1068..2264)
                     /locus_tag="AC16CB_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009370445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3341865.1"
                     /translation="MHKIAMLGTGLIGRFYTMSLLNFRGKDEIKVACAANAAHAKNFA
                     DEFGIPRWTDDMATAIRDPEIDTVIIGIPNYLHKKAALLAAEAGKAVLCTKPLATSGP
                     EALEMLEAVENAGVFHGYLEDLVYTPKTLKALDAARKGAFGKVLWARSRETHGGPHSD
                     WFWNKELSGGGAIVDMGCHCIEIARNFIGKDNRPLEVTCWADTQVHPIDAEDHAVGLV
                     RYDNGAIGQFEVSWTFRGGMDLRDEVSGTEGTVWLNHWLRTGFEMFTNVGQGGYVAEK
                     AEGDTGWLFPVGDEVGALGYVAMFLDMFDAMDTGRVPMETFYDGYIVNAIIDACYKAA
                     ETKKWEAVEIQDWRGSGLARATDTVLKDYDAERYLIKEERMPNGNLKVILKHKKTGEI
                     SQVIKE"
     gene            2386..>3297
                     /locus_tag="AC16CB_05045"
     CDS             2386..>3297
                     /locus_tag="AC16CB_05045"
                     /inference="COORDINATES: protein motif:HMM:NF013381.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR3341866.1"
                     /translation="MTSAKMTSRQRVLAALKHEEPDTVPLALGGGPYGIVDDLYLRLI
                     DLLDLGAPASPFRAGHNISYMDDRLLEKLGTDIRYCWPGLMPNSPIIPGSDENTFYDS
                     YGQVWKRALPYYYSGDGILRAATSIDDIERLVRWPDLSDPRWMAGVAERARVLNEQTD
                     YFVVMRMVASHGPFQTACDLRGTENFLKDMIRNPEFAETLLEKVTSVIENLLNLAMQA
                     SGQYFHMIELPGDDYAGNTSTIMSPVMFRKFIKPCIERLVKAVKEHDPDIQVMLHSDG
                     AITSFLPDVIDLGIDVIHPLEPLPATDL"
BASE COUNT          762 a          889 c          760 g          886 t
ORIGIN      
        1 ttccatgcga gccacgttgg ccgggtgtcc ggggaagcct tcgatctgga cgatatcgcc
       61 ttcgccgccc agtttatcag ctaaccaacg agcgctggtc atcgcccagg ccttctggtc
      121 aatgcccaca ttgtacacgc cgggcacgtt cagttcctgg tccaccgaga cgacgatgat
      181 gcctttggca accgcttctt caagggttgc gttcaaaccg ctgacatcgc cggggttgac
      241 caggatggcg tccacgtcct tggcaatcag gttctgcaac tgctggatct ggcctgccac
      301 gtccgtgtcg gcgctttcaa tgaccagctc agtagtgatg ccttgatcca tatattcctg
      361 gttgacttcg atcagctcgg caatcatctg tgtgcggtat tcgctgctga tgaatgggtt
      421 ggagatgcca atcgtgaagc cctcttccat aggttcttca ggtgctgcgg tttcttcagg
      481 tgctgcgggt acttcaggtg ccgcaggctc ctcagtggct acaggttgtt gaggggcctc
      541 ggttggtttg gggccgcacg cgccgagtag tagagttacc aaaagcaagg cgctcaaaat
      601 agtaaacaat tttttagaca tgtgttctcc tcttgtattc tctattgaaa tttttatacg
      661 atgggtgagt ttctcttctt tgaatatcac tacctccttg tgtgtatact gattattggc
      721 gtaatctcaa ccagttacca aaattctgga tgacctcaaa atggagagcg tgtaaatagg
      781 gctatcatgc cagtgtaatc aggcttcgcc tcccttgtcg tatcttagct cagttagcac
      841 tattgtacgc cataacaggt cagaatatgc gaaagtcggt tgcgcttaca cgatatgtga
      901 gaatattatg tattgtacaa ttactttgtg aattctaatc actttgtttg tatttaataa
      961 ttagagcttc gattattttc agattaaagt aggcgataaa acataaagcg ttttatcaga
     1021 cttacataag gaagatgggc aagacaggta ctttaccagt agcagtatta ttctttgatc
     1081 acttgcgaaa tttcaccagt cttcttgtgc tttaagataa ccttcaagtt gccatttggc
     1141 atacgctctt ctttaataag gtaacgctct gcatcgtaat ccttgagaac tgtatcagtc
     1201 gcccttgcca aaccgctgcc gcgccaatcc tggatctcaa ccgcttccca ttttttggtt
     1261 tcagcagctt tgtagcaggc atcaatgatc gcattgacaa tatacccatc gtagaaagtc
     1321 tccattggaa ccctgcctgt gtccattgca tcgaacatgt ctaagaacat ggccacatat
     1381 cccagagcac caacttcatc ccccacaggg aacaaccagc cggtatcgcc ttccgccttt
     1441 tcggccacgt aaccgccctg gcctacattc gtgaacatct caaaacccgt ccgtagccag
     1501 tggttcaacc ataccgtgcc ctcagtgcct gaaacttcat cgcgcagatc catgccgccg
     1561 cggaacgtcc agctgacctc gaattggcca atcgcaccgt tgtcgtaacg taccagcccc
     1621 accgcgtggt cttcagcgtc aataggatgg acttgcgtat ctgcccagca ggtcacttcg
     1681 agcgggcggt tgtccttgcc aatgaagtta cgcgctatct caatgcagtg acagcccatg
     1741 tccacgatcg cgccgccacc cgatagctcc ttgttccaga accagtcact gtgtgggccg
     1801 ccatgtgtct cgcgcgaacg ggcccagagt accttaccaa aagcaccctt ccgggctgcg
     1861 tctaatgcct tgagtgtctt gggcgtgtag accagatcct ccagataacc atgaaaaacg
     1921 cccgcgttct ctactgcttc gagcatctcc agggcttccg gcccgctggt cgccagaggt
     1981 ttggtacaca ataccgcctt tccagcctcg gcggccaaca gggcagcttt tttgtgcagg
     2041 taattgggaa tgccaataat tacggtatca atttcaggat cccggatggc tgtggccatg
     2101 tcatctgtcc aacgtggaat cccaaattcg tccgcaaaat ttttcgcatg cgctgcattg
     2161 gctgcacaag ccaccttgat ctcatctttt ccacggaaat taagtaaact catcgtataa
     2221 aaacggccga tcagacccgt ccctagcatg gcgattttgt gcatagctgt ctccttttca
     2281 tcacttgtag gaaattatat agtaacaatt ttcacacgtc aattttgtgg gcaataagat
     2341 agaattattt tatcctgaca ttttgaagga aatgagaata caacgatgac ctctgcaaaa
     2401 atgacctctc gccaacgcgt actcgcggct ctgaaacacg aggagcctga taccgttcca
     2461 cttgctctgg gtggtggccc atatggaatc gtggacgatc tctatttacg cctgatcgat
     2521 ttactcgacc tgggcgcacc tgcatctccc tttcgcgctg gacacaacat ctcatacatg
     2581 gacgaccgcc tgctggaaaa actaggcacg gacatccgct actgctggcc gggtttgatg
     2641 ccaaacagcc ccatcatccc tggttcagac gaaaatacct tctatgactc ctacggacag
     2701 gtttggaaac gtgctttacc ttattattac tctggtgatg gcatcctcag ggcagccacc
     2761 agcatagatg atattgagcg tcttgtccgt tggcctgatc tgtccgatcc tcgttggatg
     2821 gctggcgtcg ccgagcgcgc tcgtgtgctg aacgagcaga ctgattattt cgtggtcatg
     2881 cgcatggtcg cttcgcacgg ccccttccag accgcttgtg acttgcgcgg tactgaaaac
     2941 tttctaaagg atatgatacg taacccagag tttgccgaaa ccttgctcga aaaagtcaca
     3001 tccgtgattg aaaacctgct gaatcttgcc atgcaggcga gcggacagta ttttcacatg
     3061 atcgaactgc ccggcgacga ttacgcggga aacaccagca ccatcatgtc acccgttatg
     3121 ttccggaaat ttatcaagcc ctgtattgag cgcctggtca aggcagttaa agaacatgat
     3181 ccagatattc aagttatgct tcacagcgac ggcgcaatta cgagctttct acctgacgtt
     3241 attgatcttg gtattgatgt catccatccg cttgaaccac ttcccgccac tgacctt
//
LOCUS       DBNGSX010000155         5671 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_912779, whole genome shotgun sequence.
ACCESSION   DBNGSX010000155 DBNGSX010000000
VERSION     DBNGSX010000155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5671)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_912779"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="AC16CB_05050"
     CDS             <1..579
                     /locus_tag="AC16CB_05050"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007959390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3341867.1"
                     /translation="MGPSGSGKSTLMNMIGCLDQPTSGEDILDGERVSNLKDDQLATI
                     RNRKVGFVFQSFNLLPRATALANVELPLRYAGVGRGRKDRAREALELVGLADRIHHRP
                     NELSGGQQQRVAIARALVNNPAIILADEPTGNLDSKSGEEIMQLLLGLNQEHGTTLVI
                     VTHDPVVASRTPRIIQIMDGVVVEQDTGNRNQ"
     gene            576..1802
                     /locus_tag="AC16CB_05055"
     CDS             576..1802
                     /locus_tag="AC16CB_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3341868.1"
                     /translation="MNIIQSFYEALESVSSNKMRSGLTILGIVIGVAAVIAMVSVGRG
                     AQNTITGSIQGIGTNLLFVFRGGSEDVRNPKPITLGDGDAIADPFQAPSVADVAPMLQ
                     GDGIVSYAGENHFTSIIGVTPSYGPVRNTSVSEGEFIREEHILGQASVVLLGPEVAKK
                     LFNRTEGLVDETVRIEGQPFRVLGVLESKGGSAFSNQDDRILVPFSTAQTRLLRRATR
                     DRVDLLMVQAVSAQAVPQASEEVAQILRTRHRTEVGADDFTVLTQQDFLDTAQTITGV
                     LTVFLGGIAAISLLVGGIGIMNIMLVSVTERTREIGLRKALGARKIDILVQFLTESSV
                     LSLIGGIVGIMLGWLISFIVGRIAAANEADITPAIGVDTILLATLFSTAVGLFFGLYP
                     ANRAANLEPVEALRYE"
     gene            complement(1833..2714)
                     /locus_tag="AC16CB_05060"
     CDS             complement(1833..2714)
                     /locus_tag="AC16CB_05060"
                     /inference="COORDINATES: protein motif:HMM:NF024117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR3341869.1"
                     /translation="MKLPDGFIFSQSSLQDFVDCRRRFQLRYINRLAWPALETEPALE
                     AERYLQQGARFHRMVQQHLLGVPAERLSNMITDDDLSRWWHNYLSFVQALRQQSAAPT
                     EGVSEHAIFGAARGYPEISLSATLAGFRLEAKFDLVLLLPDGGTRIYDWKTSRRRPER
                     RWLANRLQTRLYPYLLTRAGAVLNGGEDLLPEKVEMIYWFADHPGQPERIAYSAQAFR
                     DDEAYLTDLVATIQRLGDDDFSLTTDLRRCAFCSYRSLCDRGARAGDLDEMQGEADIT
                     DEMALNLDFEQIGEIEF"
     gene            complement(2751..4991)
                     /locus_tag="AC16CB_05065"
     CDS             complement(2751..4991)
                     /locus_tag="AC16CB_05065"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR3341870.1"
                     /translation="MEFIPRPKQQEVLDYTGGYMGVSAVPGSGKTWTLSLLAAQIVAS
                     GELDSDQEVLVVTLVNSAVDNFYKRVSDFIQAAGLLPHLGYRVRTLHGLAHDIVRERP
                     ELVGLADNFQIIDERQVDSIRSEVAEAWLRANPNALDGYLNPELDDERLAWVRREPLP
                     NLVSEIALSFIRYAKDLQLTPERLRLQLESLPVPLSLAEMGHAIYADYQRALSYRGAV
                     DFDDLIRLALEALRLDDKLLERMRYRWPYILEDEAQDSSRLQEEILKTLAGPGGNWVR
                     VGDPNQAIYETFTTANPKYLRAFIKSRGVLGRELPNSGRSTQTIIDLANHLVKWTQRQ
                     HPVRQAREALGGPPFIEPVPEGDEGANPPDDLSKLHLVARKYSPVDEISAVVDSLQRW
                     LPDHPDDTVAVLVPRNQRGFELVDVLRRRGIEVVDSLLRSTASTRFSARVLTQLLSYL
                     SDPGSPSRLALLYKAWQRGEQAGARRERVSELLRKCGQVEDFIWPNPGQDWLEELDLE
                     DTEPQVYTQLLEFRQLVRRWQEAILLPVDQMILTASQDLFTEPSQLALAHKLAALLRQ
                     AGQSHPSWRLPELTEELAVIARNERRFLGFSDDDTGFDPGQYRGKVVVATMHKAKGLE
                     WDRVYLMSVNTYDFPSGLQGDRYISEKWFLRDSLNLEAETLAQLDALLSIDEYTWYQE
                     SEATHRARLDYVRERLRLFYVGITRAKKELVVTWNTGRRGELQPSTPLLALMDYVSSQ
                     ETSNDL"
     gene            complement(4997..>5671)
                     /locus_tag="AC16CB_05070"
     CDS             complement(4997..>5671)
                     /locus_tag="AC16CB_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341871.1"
                     /translation="QERITYLLGNRYEDLRLWLAEYAQSLDAELDHFLSRLFGELLSQ
                     PGFGFHVNYAAGEVTANLIESVQKFRWAVGDALADEGIPLGKEYLMMVQDGVIAAQYV
                     RSWQILPGDAVMVAPAYTFLMSNRPVEVQFWLDVGSRGWFERLYQPLTHPYVLSRGWG
                     QGQIWTDEHEFHASQDSLRRLVLGLVRRCRRGIYLGLSELSEQGYEERGMLLQVIQRV
                     LREGRA"
BASE COUNT         1163 a         1747 c         1648 g         1113 t
ORIGIN      
        1 atgggaccat caggctcggg caaatccacc ctgatgaaca tgatcggctg cctagaccag
       61 cccacctccg gcgaggatat cctggatggg gaacgcgtct cgaacttgaa ggacgaccag
      121 ctcgccacta tccgcaaccg caaagtgggc tttgtcttcc agagcttcaa cctgctgccg
      181 cgtgccacgg cattagccaa tgtagagctg cctctgcggt acgcaggagt agggcgcggg
      241 cgcaaggatc gtgctcgtga agctctagag ctggtggggc tggccgaccg catccaccat
      301 cgccccaacg agctttcggg tgggcagcaa cagcgcgtcg ccatcgcccg cgccctggta
      361 aacaaccccg ccatcatcct ggcggatgag cccaccggca acctcgacag caagtcgggc
      421 gaggagatca tgcaattgtt attgggactt aaccaggaac atggcaccac gctggtgatc
      481 gtcacacacg acccagtggt agcctcacgc acgccacgca tcatccaaat tatggatggg
      541 gtcgttgtgg agcaggatac agggaaccga aaccaatgaa catcatccaa tctttctacg
      601 aagccctgga gagcgtgtca tcaaacaaga tgcgctccgg gctgaccatc ctggggatcg
      661 ttatcggcgt ggcggcagtg atcgctatgg tatctgttgg gcgaggcgcc caaaacacca
      721 tcaccggctc aatccaaggc atcggcacca acctgttgtt tgtcttccgc ggtggctcgg
      781 aggatgtgcg caatcctaag cccatcacct taggagatgg agacgccatc gccgacccgt
      841 tccaggcacc ctccgtggca gatgtagctc ccatgctgca gggcgacggc atcgtctcgt
      901 atgccggcga aaatcacttc acctccatca ttggagtgac accatcctac ggtcccgtgc
      961 gcaatacctc tgtctcagag ggtgagttta ttcgagagga gcatatcttg ggtcaggcct
     1021 cggtggttct tctcggccca gaggtggcga agaaattatt caaccgcacc gaggggctgg
     1081 tagacgagac agtgcgtata gaaggccaac ctttccgggt gctcggcgtg ctggagtcaa
     1141 aaggcggctc agccttctcc aaccaggatg accggattct cgttccgttc tccaccgccc
     1201 agacccgtct gttgcgacgc gccacccgcg accgggtgga cctgctcatg gtacaggcgg
     1261 ttagcgccca ggcggtcccc caggcttctg aggaggtcgc tcaaatcctg cgcacgcgcc
     1321 accgcacgga ggttggcgcc gacgacttca ccgttcttac ccaacaggat ttcctggata
     1381 ctgcccagac catcactggt gtactaaccg ttttcctggg cggcatcgct gccatctcgc
     1441 tgctggttgg cgggatcggc attatgaaca tcatgctggt ctcggtcacc gagcgcacgc
     1501 gcgagattgg tctgcgcaaa gccctgggag cgcgcaagat cgatattttg gtgcagtttc
     1561 tcaccgaatc ctcggtgctc agcctgattg ggggcatcgt gggcatcatg ctcggatggt
     1621 tgatctcttt catcgtaggg cggatcgccg cggcaaatga agccgacatc acccccgcca
     1681 tcggcgtgga taccatcctg ctggcaaccc tcttctcaac cgcggtgggc ttattctttg
     1741 gactataccc agccaaccgc gccgccaacc tggaaccggt agaggcgctg cgatacgagt
     1801 gatgtgcagt ttgggatggt agtagttatt ggttaaaact cgatttcgcc gatttgctca
     1861 aaatccaggt tgagagccat ttcatcggtg atatccgcct ccccctgcat ctcatccaaa
     1921 tctcccgcgc gtgcgccgcg atcgcaaaga gagcgatagc tgcaaaaggc gcagcggcgc
     1981 agatcggttg tcagggagaa atcgtcgtcg cccagacgct gaatggttgc gaccaggtcg
     2041 gtcaggtatg cctcatcgtc ccggaaagcc tgtgcgctat aggcaatacg ctccggttgt
     2101 cccgggtgat cggcgaacca gtaaatcatc tcaaccttct cgggcagtag atcctccccc
     2161 ccattgagaa cagcaccagc acgggtaagc aggtaggggt acaggcgcgt ttgcagacgg
     2221 ttagccagcc agcgccgttc aggcctgcgc cggctcgtct tccaatcgta aattcgagtg
     2281 cccccatccg gcagcaatag caccaggtcg aacttggctt ccaggcgaaa accagctagt
     2341 gtcgcagaca gactgatctc cggatagcct cgcgctgcac cgaagatggc atgctcagaa
     2401 accccctctg taggagccgc actctgctgg cgcaacgcct ggacgaagct caggtagtta
     2461 tgccaccagc ggctcaggtc atcgtcagtg atcatgttag atagacgctc cgctggcaca
     2521 cccaagagat gctgctgcac catgcgatgg aaacgcgcac cctgctgcag gtagcgttca
     2581 gcctcaaggg ctggctccgt ctctagggct ggccatgcca ggcggttaat gtagcgcaac
     2641 tgaaagcggc gacggcagtc cacaaaatct tgcaagctgc tctggctaaa gataaaacca
     2701 tcaggcagtt tcacgggtca gtctccagac atcttgaccg atcgagataa tcacagatca
     2761 ttggatgttt cctgggagct cacgtagtcc atcaaggcta atagcggcgt ggatggttgc
     2821 agctcgcctc tcctgccagt gttccatgtg accactagct cttttttggc gcgcgtgatg
     2881 cccacataga acaagcgcag ccgttcacgc acataatcca ggcgggcgcg gtgggtggcc
     2941 tcgctctcct ggtaccaagt gtactcatcg atcgagagca atgcatccag ctgcgccaac
     3001 gtctcagctt ctaaattcaa gctatcgcgc agaaaccact tctctgagat gtagcgatct
     3061 ccctggaggc cggaggggaa atcataggtg ttgaccgaca tcaggtagac acggtcccat
     3121 tccaagccct tggccttgtg catggtggcg accaccacct tgccccgata ttggcccggg
     3181 tcgaagccgg tgtcgtcatc gctgaaaccc aggaaacggc gctcattgcg agcgatgacc
     3241 gccagctctt cagtcagctc gggcaagcgc caggaggggt gcgattggcc tgcctggcgc
     3301 agcagcgccg ccagcttgtg tgccaatgcc aattgtgacg gctcggtaaa caggtcttgc
     3361 gaggcggtga ggatcatctg atctacaggc aggaggatgg cttcctgcca gcggcggacc
     3421 aattggcgaa actccaggag ctgagtatag acctgtggtt cagtgtcctc cagatccagc
     3481 tcttccaacc aatcttgccc cgggttaggc cagataaagt cctccacctg tccacactta
     3541 cgcagcagct cggagacgcg ctcacggcgg gcacctgctt gctcaccgcg ctgccaggct
     3601 ttatacaata gggccaggcg agatggcgag cccggatcgg agaggtagct gagcagttga
     3661 gtgagcaccc tggcagagaa gcgggtcgag gccgtgctgc gcaacaagct gtccactacc
     3721 tcgatgcccc ggcggcgcaa aacgtccacc aactcgaagc cgcgctggtt gcgcggcacc
     3781 agcacggcta ccgtgtcatc tgggtgatct ggcagccagc gctgcagcga atcgactacc
     3841 gccgagattt cgtctacagg gctgtatttg cgcgccacca agtgcaactt agatagatca
     3901 tccggggggt tggcgccttc atcgccctcg ggcaccggct cgatgaaggg cggtcctccc
     3961 aaggcctcgc gagcctggcg aactgggtgc tggcgctggg tccacttcac caggtgattt
     4021 gcgaggtcga ttattgtctg tgtggagcgg cctgagttgg gcagctcccg ccccaaaacg
     4081 ccgcgactct tgatgaaagc gcgcagatat tttggattgg cggtggtaaa agtctcgtag
     4141 attgcctggt tcgggtcgcc cacgcgcacc cagttgcccc ccgggccggc cagggtcttg
     4201 agtatctcct cctgcaggcg gctgctgtcc tgggcttcgt cctccagaat atatggccag
     4261 cgataacgca tccgctccag caacttatcg tccagacgca acgcctccag ggccaggcgg
     4321 atgaggtcat caaagtccac cgcgccgcgg taggacaagg cgcgctggta gtcagcatag
     4381 atggcgtgac ccatctcagc caggctgagc ggcacgggca ggctctccaa ttgcaatctc
     4441 agccgctcgg gggtaagctg aaggtctttt gcatagcgga tgaagctcag ggcaatttcg
     4501 ctgaccaggt tagggagcgg ctctcggcgc acccaggcca aacgttcgtc atccagctca
     4561 gggttcaggt agccatccag tgcattgggg ttggcgcgca gccatgcctc agccacctca
     4621 ctgcggatgc tgtccacctg gcgctcgtca atgatctgga agttatccgc cagccccacc
     4681 agctcagggc gctcgcggac gatatcgtgc gccagaccat gcaatgtgcg cacgcggtag
     4741 cccaggtgcg gaagaagccc ggcggcctgg atgaagtcgc tgacacgctt atagaaatta
     4801 tccaccgcgg aattgaccag cgtaaccacc aagacttcct ggtcgctgtc cagctcgccg
     4861 ctggcaacga tctgagctgc cagcagggag agcgtccagg tcttgccgct gcccggcacg
     4921 gcagagacac ccatgtagcc gccggtgtag tcgagtactt cttgctgctt ggggcgtgga
     4981 atgaactcca tgttttctac gccctgccct ctcgcagaac acgctgtatc acttgcagca
     5041 gcatgccgcg ctcctcgtag ccctgctcgc tcagctcgct cagccccaga tagatacccc
     5101 ggcggcagcg gcgcaccaga cccaacacca ggcggcgcag ggaatcctgg ctggcatgga
     5161 actcgtgttc atccgtccag atttgcccct gcccccagcc ccggctgagc acgtaggggt
     5221 gggtgagcgg ctggtacagt cgctcgaacc aaccgcggct gcccacatcc agccagaact
     5281 gcacctcgac cgggcggttg ctcatcagga aggtataggc tggggctacc atcacggcat
     5341 cgcctggcag tatctgccag ctacggacat attgggctgc gatgacacca tcctgcacca
     5401 tcatcaggta ttccttgccc aaggggatgc cctcatctgc cagagcatct cccaccgccc
     5461 agcggaactt ctgcaccgac tcgatcaggt tggcggtgac ctccccggcg gcgtaattca
     5521 cgtggaagcc gaagccgggc tgcgagagca actcgccaaa caagcggctt agaaagtgat
     5581 ccagctcagc atcgaggctt tgggcatact ccgccagcca caagcgcaaa tcctcgtagc
     5641 ggttgcccag caagtaggtg atgcgctcct g
//
LOCUS       DBNGSX010000156         4284 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_704538, whole genome shotgun sequence.
ACCESSION   DBNGSX010000156 DBNGSX010000000
VERSION     DBNGSX010000156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_704538"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..931
                     /locus_tag="AC16CB_05075"
     CDS             170..931
                     /locus_tag="AC16CB_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR3341872.1"
                     /translation="MSEQTASDEPIIIARDVHKWYGHFHALKGINMDVMRGEVIVIFG
                     PSGSGKSTFIRTINRLEEHQRGQIIIDGIELTHDTRNIEKVRAETGMVFQQFNLFPHL
                     TVLQNVALAPIWVRKWKKQKAEEVGMQLLERVGIPEQAAKFPGQLSGGQQQRVAIARA
                     LAMQPKIMLFDEPTSALDPEMIKEVLDVMIGLAKTGGMTMIVVTHEMGFARGVADRMF
                     FFDEGLIVESGTPDDIFKNPQEDRTKLFLSQILTH"
     gene            complement(1013..1486)
                     /gene="rnhA"
                     /locus_tag="AC16CB_05080"
     CDS             complement(1013..1486)
                     /gene="rnhA"
                     /locus_tag="AC16CB_05080"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015142759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HIR3341873.1"
                     /translation="MGDELKQIQIYTDGACEGNPGPGGYGVILIYARHRKELSGGFRL
                     TTNNRMEVYAAIAGLRSLKEPCKVTLYSDSEYLVKAMTLGWVQRWKAKNWKRNKREKA
                     LNVDLWEQLLVECERHQVEFVWVKGHAGQPENERCDQLSMNALRRGELPIDEGYE"
     gene            complement(1498..1989)
                     /locus_tag="AC16CB_05085"
     CDS             complement(1498..1989)
                     /locus_tag="AC16CB_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341874.1"
                     /translation="MNHKILVSLGLCLLALALAACAGLSDPVATEPTIDPSEPISNDE
                     QPSGEAPISPLDPIPGEDKMRRGEVVIEKSEVLIMESYPLQVMLSIQGTLSTPCHHLR
                     AKLEKPDGENRIQVEVYSLVDPNEICVQMLEDFETNLPLGSYPDGSYTIWLNGVQVGE
                     FKQ"
     gene            complement(2041..3492)
                     /locus_tag="AC16CB_05090"
     CDS             complement(2041..3492)
                     /locus_tag="AC16CB_05090"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017720266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HIR3341875.1"
                     /translation="MVNLSDLKKINDYEWEIPKSFRSDMRVPVRIFATRRLLEAVMDD
                     RSLEQAVNSATLPGLVGHVVVMPDMHQGYGFPIGGVAATRYPEGVISPGGIGYDINCG
                     VRLLASQIEYDAVADQMSALASALNNYCPSGVGTKGHVRVSEAELDEVCRDGSRWAQK
                     KGFATEMDVRRTEENGRLEGADPNKVSKRAKERGRAQIGTLGAGNHFIEVDLVEKVFD
                     AQAAQAMGLTEGYLAVQIHCGSRGFGHQICTDYVNEFQRAVHRYGIKLADRELVCAPL
                     NSPEGEAYLAAMRCAANYAFANRQVLAFYARKAFEEVLAGRVKNWHLHQVYDIAHNMG
                     KIETHEIDGERIKVCVHRKGATRAFGPGFEGLPGEFRAIGQPVLVPGSMGTASWVLVG
                     TQGSMERSFGSTCHGAGRVMSRAQAKKKVWGEDLRNQLESGGIHVRAGSMAGLAEEAP
                     QAYKDVDDVVETVSGAGIARKVARLVPVAVVKG"
     gene            complement(3608..4021)
                     /locus_tag="AC16CB_05095"
     CDS             complement(3608..4021)
                     /locus_tag="AC16CB_05095"
                     /inference="COORDINATES: protein motif:HMM:NF014058.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0072669 - tRNA-splicing ligase complex
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HIR3341876.1"
                     /translation="MPTPSAGFVERPHTADWELAVWAPDLPTLFEQAARGMYMLAGLR
                     LQEVPHLKRRLELQAIDDESLLVQFLDELLYLQGVEGIAFEKFQLQITGNELKAELDG
                     APVASLDKEIKAVTYHNMKIQAGPCGLEVRIVFDV"
     gene            complement(4146..>4284)
                     /locus_tag="AC16CB_05100"
     CDS             complement(4146..>4284)
                     /locus_tag="AC16CB_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_201960451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HIR3341877.1"
                     /translation="TGQFATRAQARTTIFEYIEVWYNRHRLHSSLGYLSPVEFEQFSG
                     H"
BASE COUNT         1023 a         1278 c         1014 g          969 t
ORIGIN      
        1 gtttatcgga ctgcaaatgg aggtttatct atttgtcgcg ttagtatttt ggattttcag
       61 ttacctcatg tcgtatgcca gccagcgact ggaagttgcg cttggcgttg gtgagaggta
      121 aaataaatta cagaactaca tattatcagt gatagatttg gagaagctaa tgagtgaaca
      181 aacggcatcg gacgaaccta tcatcatcgc cagagatgtg cataaatggt atggtcactt
      241 ccacgccctg aaaggcatca atatggacgt gatgagggga gaagtgatcg tcatcttcgg
      301 cccatcaggt tcgggcaaat cgacatttat ccgaacgatt aatcgcctgg aagagcacca
      361 gcgaggccag attatcatcg atggtatcga gctcactcat gacacgcgta atattgagaa
      421 agtacgggcg gagacgggaa tggtcttcca gcagttcaac ctttttcccc acctcaccgt
      481 gctgcagaac gtcgctctgg cacccatctg ggtgcgcaag tggaaaaagc aaaaggctga
      541 agaagtaggc atgcagctcc tggaacgcgt ggggatcccg gagcaagcgg ccaaattccc
      601 tgggcaactc tccggcggtc agcagcagcg cgtcgctatc gcccgcgccc tggcgatgca
      661 gcccaagatc atgctcttcg acgagcccac ttccgccctc gatccagaga tgatcaaaga
      721 agtgctggac gtgatgatcg gactggccaa gaccggcggc atgaccatga tcgtggtcac
      781 tcacgagatg ggtttcgcca gaggcgtggc tgatcgcatg ttcttcttcg atgaaggctt
      841 gattgtagag agcggcacac cggacgatat cttcaagaac ccccaggaag atcgcacgaa
      901 gctgttctta tcgcaaatcc tgacgcatta gtcaaaattg cagcaatcgc aagctgaaaa
      961 ccacacgccc caatcgtgca ttggggcgtg tggttttcag tcaaagaatt tattattcgt
     1021 aaccttcatc gataggcaat tcacccctgc ggagcgcgtt catcgagagc tgatcacacc
     1081 gttcattctc gggttgtccg gcatgcccct tgacccacac aaattccact tggtggcgct
     1141 cgcactccac cagcaattgc tcccacaaat cgacgttcag agccttctcg cgcttgttgc
     1201 gcttccaatt cttggccttc cagcgctgca cccaacccag ggtcatggcc ttgaccaggt
     1261 attcagaatc gctgtataag gtcaccttgc agggttcttt aagagaccgt agacccgcaa
     1321 ttgctgcata gacctccatg cggttattgg ttgtcaaccg gaacccacca gatagctctt
     1381 tacggtgcct agcgtagatc aaaataacgc catagcctcc cgggccagga ttgccctcac
     1441 aggctccatc agtatatatt tgaatctgtt tgagctcatc acccataaaa aaccggccta
     1501 ctgcttgaac tcaccaacct gcaccccatt gagccagatc gtgtagctgc cgtctgggta
     1561 gctacccaag ggcagattcg tctcgaagtc ctccaacatc tgtacgcaga tctcgtttgg
     1621 atctaccagc gaatacacct cgacctggat gcgattctcg ccatctggtt tttcgagctt
     1681 ggcgcgcagg tggtggcagg gggtcgagag cgtgccttgg atgctcaaca tcacctgcaa
     1741 tgggtagctt tccatgatca gtacctcgct cttctcaatg accacctcgc cgcgcctcat
     1801 cttatcttcg ccgggtatgg ggtcgagcgg agagataggc gcttcacccg agggctgctc
     1861 atcgttggaa atcggttcgg aggggtcaat agttggctct gttgccaccg gatcgctcaa
     1921 tcctgcgcag gctgccaacg ccagagcaag caaacataac ccgagtgaaa ccagtatctt
     1981 gtgattcatc tccacctctt aatatgctat ccagaatctc tgaaaccccg cacccgaatc
     2041 tcagcccttg actactgcca cagggaccaa gcgagcgacc tttcgagcga tgccagcccc
     2101 tgaaacggtt tcaacgacat catccacatc tttataggct tgcggggctt cttccgccaa
     2161 tcccgccatc gagcctgctc gcacgtggat gccgccggat tccaactggt tgcgcaagtc
     2221 ttcgccccac actttcttct tggcctgtgc ccggctcatc accctgccgg ctccgtggca
     2281 agtggagcca aaggaacgct ccatgctgcc ctgtgtccct accagcaccc aagaggctgt
     2341 ccccatacta ccaggcacga gcaccggctg cccaattgcg cggaattcgc ccggcaagcc
     2401 ttcgaaacca gggccgaaag cgcgggtcgc acccttgcgg tgcacacaca ccttgatgcg
     2461 ctccccatcg atctcatggg tctcaatctt tcccatattg tgggcgatat cgtaaacctg
     2521 gtgcaagtgc cagtttttca ctctacccgc caggacctcc tcaaacgctt tcctggcata
     2581 gaatgccagc acctgccggt tagcaaacgc gtagttggct gcacagcgca tggctgccag
     2641 gtaagcctct ccctcaggcg agttcagcgg tgcgcatacc agctcgcgat cagccagttt
     2701 aatcccataa cgatgcaccg ctctctgaaa ctcgttgaca taatcggtgc aaatctggtg
     2761 accaaagccg cgcgagccgc agtgaatctg cacagctaga taaccctcgg tcaatcccat
     2821 ggcctgagca gcctgggcgt caaacacttt ctccaccaga tccacctcga taaaatggtt
     2881 gcctgcccca agtgtgccga tctgggcccg gccccgctct ttggcgcgct tgctcacttt
     2941 gtttgggtca gcaccttcca ggcggccatt ttcctcagtg cggcgcacat ccatctcggt
     3001 agcaaaaccc ttcttctgcg cccagcgact gccatctcgg catacctcgt ccaactcagc
     3061 ttccgaaacg cgcacgtgcc ctttcgtacc taccccgcta ggacaataat tgttcaaagc
     3121 agaggctaac gcactcatct ggtcagcgac agcatcatat tcgatctgag acgccaataa
     3181 gcgcacgccg cagttaatat cataaccaat cccaccaggt gagatcaccc cttctgggta
     3241 acgcgtagcc gccacgccac caattgggaa accatagccc tggtgcatat ccggcatcac
     3301 cacaacatgt cccaccaagc ccggtagcgt agcagagttt accgcttgct ctagcgaccg
     3361 atcatccatt accgcttcta acaagcgccg cgtagcaaag atgcgcactg gaacacgcat
     3421 atcgctgcga aaagatttcg ggatttccca ttcataatca ttgattttct ttagatcact
     3481 caagttaacc atcactacac ctcttgccca ggtgaagggc aaatgaccac atcaacaaat
     3541 gtcaaacgaa tctcgcaaag cccccttctc agctcttgta caccgatccc atccagatcg
     3601 cttgctgtca aacgtcaaag acaatcctca cttccagccc gcacggtcca gcctggattt
     3661 tcatgttatg gtacgtcacc gctttaatct ctttatccag ggatgccacc ggcgcaccat
     3721 ccagctctgc cttcaattca ttgcctgtta tctgtagctg aaatttttca aacgcaatcc
     3781 cttccacgcc ttgaagataa agcaactcat ctagaaattg caccaagaga ctttcatcgt
     3841 caatagcctg caattcgagc cgacgcttga gatgcggcac ttcctgcaag cgcaggcctg
     3901 ccagcatgta catgccgcgc gccgcctgtt caaacaaggt aggcaagtcg ggtgcccaga
     3961 ctgccaattc ccagtcagca gtgtggggcc tctcaacaaa accggctgaa ggtgtcggca
     4021 tagaattatt atacctaaat cacagaagca tagctgctca tcccagggca attgcatatg
     4081 ccctgatggg catgcgcaat cccgtaaaaa aagtgtttga ccttgccccg aaaaagtgga
     4141 cacgattaat gtccggaaaa ttgttcaaac tccacagggc tgagatagcc caaggaagag
     4201 tgaagccgat gacggttata ccacacctcg atgtactcga agatagtggt ccgtgcttga
     4261 gcccgagtgg caaattgtcc agta
//
LOCUS       DBNGSX010000157         4105 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1812845, whole genome shotgun sequence.
ACCESSION   DBNGSX010000157 DBNGSX010000000
VERSION     DBNGSX010000157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1812845"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(384..1409)
                     /locus_tag="AC16CB_05105"
     CDS             complement(384..1409)
                     /locus_tag="AC16CB_05105"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR3341878.1"
                     /translation="MDKEQVLQSNLGEYSRCDKHKQKTDHRTIISKTQKTDAALKEKI
                     RNALWNDKVLRALDDNEIDVQVINQVVYLEGHIVSSSSLNRIINALHTISGILGIKNY
                     LVLDDKLSSQIASSLGMLEHNNDCKFFTGVTHGVVVLNGQVNSKNLRKKAEQCAANNP
                     KVRGVINYIRVPGVELDIQDHRFLQPSIGEEIKFLDGISGTVRKVIINPDNRRVVAMI
                     IQGHFYGVMRNLPLSNLNASQSEEQIIVIPMVATRHVNERSIFLNINSSDFTKYPEFD
                     PSKFIVPNKDWLPPYPYCPDDILIPVKFQPVDKDIAGNPIQASSTHITGIPKWMEELL
                     YNDSLGG"
     gene            complement(1479..1613)
                     /locus_tag="AC16CB_05110"
     CDS             complement(1479..1613)
                     /locus_tag="AC16CB_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011715667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341879.1"
                     /translation="MAKSQKNKKDSKKKPLRTKKEKRQAKRDKKNNNNDLITSINQTN
                     "
     gene            complement(1733..2122)
                     /locus_tag="AC16CB_05115"
     CDS             complement(1733..2122)
                     /locus_tag="AC16CB_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017301671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341880.1"
                     /translation="MKKEVGLWIDHREAFLVTLTDNMEELNRIYSKIEVDAQFSGVSR
                     SAGSQEAENIQDRKYLNNLEKYYDVVIGYIRDADSILIFGPGEAKGELAKSLKNKNLS
                     QHIVSIETSDKLTEPQIAAKVREYFPR"
     gene            complement(2374..2694)
                     /locus_tag="AC16CB_05120"
     CDS             complement(2374..2694)
                     /locus_tag="AC16CB_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341881.1"
                     /translation="MNRETIGKWAFIIGLVIAVLAGLFFQPGWAIWVLAVLGVIVGLL
                     NVTAEETRGFLLASIALTISATALNSLPIIGTAFSYVLPFIVAFVAGGMIVVALKELF
                     QTAR"
     gene            complement(2795..3649)
                     /locus_tag="AC16CB_05125"
     CDS             complement(2795..3649)
                     /locus_tag="AC16CB_05125"
                     /inference="COORDINATES: protein motif:HMM:NF013324.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR3341882.1"
                     /translation="MRTIYGPIFTLRPYERGIQEKLGKYARFVMPGLGFQFPFIQITR
                     VRDIREHTMDIHPQPVITKDNVEILVDGIMWARPTIEEEAIKRTFYNIDDWKRAIIQL
                     AMTNLRQEFGDLTLDESLVARETIATNLQRILNTYAIEWGLTVNKVEIRLIDPPEDIK
                     KAMHKQKTAEQERRSMRLLATGEFEAAEQQKLAIIQRAEGEREAAIYVAQGKAEAIRL
                     VNEAAEKYFIGNAQTLKQLEMVENSLRENAKVVITENGISPTLLLGSLPVSLSSLPVN
                     LSSEKGIK"
     gene            complement(3698..3928)
                     /locus_tag="AC16CB_05130"
     CDS             complement(3698..3928)
                     /locus_tag="AC16CB_05130"
                     /inference="COORDINATES: protein motif:HMM:NF039818.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5661 family protein"
                     /protein_id="HIR3341883.1"
                     /translation="MGDALGINWQAFNVEQFQIGLEVELEHGASDTQTNATGDDALNT
                     GKIALAHLKEFPDYYTHLSAMEKEADEYWANV"
BASE COUNT          960 a          945 c          862 g         1338 t
ORIGIN      
        1 cgcagcgttg gcaactctca gaaagccatt gactctctgc gccgcacctg ctccgttctc
       61 agaggtccga gtcgccttgc aagctagcta tttcatcttc cgtccgcgat tattagtctt
      121 ggattcaact cgtaagtgca acaggtacat tctctttaaa gaactcttga aaaggagaat
      181 tgaaggcgag actgtggtgg acccatcatt gtttgttggg gggatatcta taagccttat
      241 taataccccg atgacacctt acgctaacag gatgttttcg gtataagttt acttcacgca
      301 tattcacgca tattattttt ttcttcatca tgttttttat gcgtttttaa tgttagtcat
      361 cgtttaatgg ttattctcct taactatccg cccaggctgt cattatatag gagttcttcc
      421 atccatttcg ggattccagt aatgtgtgtt gatgatgcct ggatcggatt gcccgctata
      481 tctttatcta cgggttggaa cttaaccgga attagaatat catcgggaca gtaggggtat
      541 ggcggtaacc aatctttatt cgggacgatg aatttggatg ggtcaaattc tggatacttt
      601 gtgaagtctg agctattaat gtttaggaaa attgaccttt cattcacatg ccttgtggca
      661 accatcggaa taacaattat ttgttcttct gattggctag cgttgagatt cgataacggg
      721 agatttcgca ttaccccata aaagtgccct tgaattatca tggcgactac tcgacggtta
      781 tctgggttga tgataacttt tctaactgtt ccagaaatgc catcaaggaa ttttatttcc
      841 tcgccaatgg atggttgtaa aaaacggtga tcttgaatat ccaattctac cccgggcaca
      901 cgaatgtaat tgataacacc acggactttg gggttattgg cggcgcattg ttctgctttt
      961 ttacgtaaat tcttgctgtt cacttgaccg ttcagcacca ctacgccatg tgtgactcct
     1021 gtgaaaaact tacagtcatt attgtgttcc aacatcccta atgatgaagc aatttggctg
     1081 gatagtttgt catccaaaac gagataattc ttaattccta agattcctga aatggtgtgc
     1141 aaggcgttta taatccggtt caggctggat gaactcacaa tatgtccttc aaggtagaca
     1201 acctgattta ttacctggac atcaatttcg ttatcatcca gagctctgag aaccttatca
     1261 ttccataagg cgttcctaat tttttccttt aatgccgcat ccgttttctg ggttttacta
     1321 ataattgttc tgtgatctgt cttttgctta tgtttatcac accgggaata ttcacccaaa
     1381 tttgattgca gtacctgttc cttatccatt tgtgtttctc tcaagactat cttttggcgg
     1441 attttccata taaaccagca aaggttatat ttgattggct aatttgtttg attgattgat
     1501 gttatcaagt cattattatt gtttttctta tcccttttgg cttgtctttt ttccttttta
     1561 gttcttagcg gtttcttctt agaatccttt ttattcttct gacttttagc catgattttt
     1621 ctccttaggt ggtttatttt taggttagat tgcagctatg aatttgtgtt tgttagttga
     1681 aactgggact tattcttaat tccgagacat tctttacttc ttgtggcaac tgctatcttg
     1741 gaaaatactc tcggacttta gccgcaattt ggggctctgt gagcttgtca ctagtctcaa
     1801 tgcttacgat atgttgactt aagtttttat ttttcagact ttttgcgagc tcgcctttgg
     1861 cttcaccagg tccgaagatc aggattgagt ctgcatctcg aatataccca atgacgacat
     1921 catagtattt ctctagattg tttaaatatt ttctgtcctg aatgttttcc gcttcctggg
     1981 atcctgctga ccttgagacc ccagaaaact gggcatctac ttcaattttt gagtagatcc
     2041 gattcagttc ctccatatta tcagtgagtg tcacgaggaa cgcctctcga tggtcaatcc
     2101 acaatcccac ttctttcttc atggatatgg ttcctttctt tcttccttgt cacattgatt
     2161 ggctggctgc cttctggcag cgttgtcgtg ctacgggtct gcgtcggctc agacatgttt
     2221 ttgtaaggcg aattcataga gcccgaagat ggttgtcaat aaacctttcc gaacgtcacc
     2281 aacacctaat acaaaatgcg ctttgcctta tcgatagcag gtgggaaata taccattgcc
     2341 tgctatcgat aattgggcag ttggctagga ttattatcga gcggtctgga atagttcttt
     2401 caatgcgacc acaatcattc caccagcgac aaaggctaca atgaacggca ggacatagga
     2461 aaaagctgtt ccgataatgg gtagagagtt taacgccgtt gctgaaatag taagtgcaat
     2521 cgaagccaac agaaagcctc gtgtttcttc ggcggtgaca ttgagcagac ctacgatcac
     2581 accaaggacc gccagcaccc agatagccca tccgggttga aagaacagcc cggcgagtac
     2641 ggcaataacc aggccaatga taaaagccca tttcccgata gtttcacgat tcattttagt
     2701 ctcctttcac taacgaatag caagtccctc ttcgaaggtt atgttttggt tcctcaaaga
     2761 ggataattta acttactgga acattattct caatttattt gattcctttc tcgctggata
     2821 agttcaccgg caagctggac aagctgaccg gcaagctacc cagcaacaga gtcgggctga
     2881 tcccattctc ggtgataacg actttggcat tctcacgcag cgaattttca accatctcca
     2941 gctgcttgag ggtctgcgcg ttgccgataa agtatttctc agcagcttca ttgaccaggc
     3001 ggatggcttc agctttgcct tgagctacat aaatcgctgc ttcacgttct ccctctgccc
     3061 gctggatgat ggccagtttt tgctgttcgg ccgcctcaaa ctcacccgtt gctaatagac
     3121 gcatggaacg ccgctcttgc tcggcagtct tttgcttgtg catggctttc ttgatatctt
     3181 cgggtggatc aatcaggcgg atttccactt tgttgacagt cagtccccat tcgatagcat
     3241 aagtgttcaa gatgcgttgt aaatttgtgg caatcgtctc gcgcgccacc aggctctcgt
     3301 ccagagtcaa atccccaaac tcttgtcgca aattggtcat cgccagttgg ataattgctc
     3361 gtttccaatc atcgatattg taaaatgtgc gcttgatcgc ctcttcctcg atggtcggac
     3421 gcgcccacat tattccatcg accaggattt cgacgttatc tttggtgatc accggctggg
     3481 ggtgaatatc catcgtgtgt tcgcggatat cacggacgcg ggtaatttgt atgaacggga
     3541 attgaaatcc caagccgggc atcacaaacc gtgcgtactt tcccagcttt tcctggattc
     3601 cccgttcgta aggacgcaag gtaaagattg gaccatagat tgttctcatt gcattctcct
     3661 tattcaaccc ttcgattttg caactgcctg gatggtgcta cacatttgcc caatattcat
     3721 cggcctcttt ttccattgct gagaggtgtg tgtagtaatc cgggaactct ttgagatgcg
     3781 caagagcgat tttcccagtg ttcaaagcat catcaccggt tgcattggtc tgtgtatcgc
     3841 tggcgccatg ctcaagctcc acctcgagcc cgatttggaa ctgttccaca ttaaacgcct
     3901 gccaattgat tcctaaagca tcaccaattt gttgcttcgc ctcagcgagt ctttaccgtt
     3961 tttctgcgca catttataga tctccttatc caggttcttc cctggcactc tacgctgatt
     4021 ccatagcaca agtcactgtg tggttgaggt gcttttttgg ggcacagcag gaagccaatc
     4081 atacctgctg tgccccaaat atgat
//
LOCUS       DBNGSX010000158         1908 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1674642, whole genome shotgun sequence.
ACCESSION   DBNGSX010000158 DBNGSX010000000
VERSION     DBNGSX010000158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1674642"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="AC16CB_05135"
     CDS             <1..377
                     /locus_tag="AC16CB_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3341884.1"
                     /translation="TTTTGGGALACSAAIASIHVTLRDRMWEQAAEKGDYLIPKLKEL
                     AARYPQIFEDITGKGLLIGMHFHNPETGYKIAAGLFKRGVLVAGTLTSAQTVRIEPPL
                     VITYDQINTVLDRLEDTLREVS"
     gene            466..1671
                     /locus_tag="AC16CB_05140"
     CDS             466..1671
                     /locus_tag="AC16CB_05140"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003987887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HIR3341885.1"
                     /translation="MKVLLVGVGGVGEAIAAIAKNRSWIEQIVLADYNLERSREVQAR
                     LNDKQRFPVEWVDASQQDQIEALASKYQVDMIMNACDPIFNVPIFEAAYNYGCNYMDL
                     AMTLSEPHPEKPYELCGVKLGDYQFERAGLWEKKGLLALVGLGVEPGMANVFARYAQD
                     YLFDEIDEIGVRDGANIEVRGYEFAPNFSIWTTIEECLNPPVIWEKKRGWFTTEPFSE
                     PEIFEFPDGIGSIEVVNVEHEEVLLIPRWVKCNRVTFKYGLGDQFIDVLKTLRMLGLD
                     NKEPIKVKDVMVAPRDVVAACLPDPAHMGDLMSGKTCAGTWVKGVKDGKQRQVYLYQV
                     ADNEECMKTWGCQAVVAQTAFNVVIGWDLLVHGVWQGEGILGPEAFDPLPFMNKMAEY
                     GFPYGIKEM"
BASE COUNT          513 a          356 c          543 g          496 t
ORIGIN      
        1 ataccactac tactggaggg ggtgccttgg cgtgcagcgc ggcgatagcc tcgatccatg
       61 tcacattgcg cgatcgaatg tgggagcaag ctgctgaaaa gggcgattat ctcatcccaa
      121 aattgaagga actggctgcg cgttatccgc aaatttttga ggatattacc ggcaaaggct
      181 tattgatcgg catgcatttt cataatccgg aaacgggata taagattgca gctggtctat
      241 ttaagcgagg agtgcttgtg gctggaacgc tgaccagtgc tcaaaccgta cgaatcgagc
      301 cgcctctggt gatcacctat gatcagatta acacggttct ggaccggctc gaggatacac
      361 taagagaggt gagttaagtg caatgagatt gaacggatat tttttatttt ttggagccat
      421 gcctctagag acattagctg agagcatgaa aatcggaggg agaatatgaa agttcttttg
      481 gtcggtgtcg ggggggttgg agaagctatt gccgccattg ctaaaaatcg atcttggatc
      541 gagcagattg tgttggcaga ttacaatctg gagcgatcga gagaggttca agcgaggttg
      601 aatgataagc aacgattccc ggttgaatgg gtggatgctt cgcaacaaga tcagattgaa
      661 gccttggcga gtaaatacca agtagatatg atcatgaacg cctgtgatcc aatcttcaat
      721 gtgcccattt ttgaggctgc gtacaactac ggctgtaatt acatggattt agcgatgacg
      781 ctatctgaac cgcacccaga aaaaccctat gagttatgtg gtgtgaagct gggtgattac
      841 cagtttgagc gtgcaggctt gtgggagaag aagggcctgc tggccttggt gggcttgggt
      901 gtggaaccag gaatggctaa tgtgtttgct cgctatgctc aagattacct cttcgatgag
      961 attgatgaga tcggagtgcg tgatggcgca aacattgaag tgcgcggtta cgaatttgcc
     1021 ccaaatttct ccatctggac aacgatcgaa gagtgtttaa atccgccagt catatgggaa
     1081 aagaagcgag gctggtttac taccgaacca ttctctgagc cggagatatt cgaatttcct
     1141 gatggaattg gttcgataga ggtcgtcaat gtagagcacg aagaagtgtt gcttatacct
     1201 cgctgggtca agtgtaaccg tgtaaccttt aaatatgggc ttggcgatca atttattgac
     1261 gtgctcaaaa cgctacgtat gttgggatta gacaacaagg aaccgatcaa ggttaaagat
     1321 gttatggtag cgccgcgaga tgtggtcgcg gcatgcttgc ctgatccggc acatatggga
     1381 gatttgatga gcggtaaaac atgcgctggt acctgggtta aaggagtcaa agatggcaag
     1441 cagcgtcagg tatatctcta ccaggttgct gacaatgaag agtgcatgaa gacctgggga
     1501 tgccaggctg ttgtggcgca aacggcgttt aacgttgtca ttggatggga tcttctggtg
     1561 catggggtgt ggcaagggga agggattctt ggaccggagg cgtttgatcc attgccattc
     1621 atgaacaaga tggcagaata cggatttccg tatggtatca aggagatgta gactgggatt
     1681 tgtctgcaga ttttgtggac aagggagttg acacctctaa ttgaagatcg aataggcgca
     1741 tcattgctct ctattcctgt tcaggcaaat gccttttcaa tgcatgaaaa acgagtaaaa
     1801 tcgaaaggat aaataataac ctttagaaga tgaatatgcc ccaatatgca gttgaaattg
     1861 acgcaaaact tgtcccattg cgggatcaat tatggataca ggctgctg
//
LOCUS       DBNGSX010000159         4682 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_704581, whole genome shotgun sequence.
ACCESSION   DBNGSX010000159 DBNGSX010000000
VERSION     DBNGSX010000159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_704581"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="AC16CB_05145"
     CDS             complement(<1..1079)
                     /locus_tag="AC16CB_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341886.1"
                     /translation="MRFRRISHLFILILFILYPTGMVLSAPENEPESPDAWSAVAPGI
                     EYQKYHVGKPTYPRDINIFVARMDRSNLGVAIESSIAQGRLSGGTETIGGMAARYDQA
                     INYWGETWGNRNRVVVAINGYFFGYPEELPGVPWSGQIHSGWYAKRYSETVGDAGFVW
                     KLNREAFIGKCVDRHPGEKNDIFFLGIAYDPNIDAINVARSDEQLILYTPQYDATTKT
                     LENGATPRVEILIELTRPGLLVSDPSYVRGYIREIRRNNGSTPIPFDHVVLAAWGDVG
                     SAMVSRINSDDIALGNEVRITQEIRDCPSETIKHDWSKAYAGIGGDYHFLKDGGYYAP
                     NNPDATWPNSRTVIAYNASYVYYIVV"
     gene            complement(1235..2509)
                     /locus_tag="AC16CB_05150"
     CDS             complement(1235..2509)
                     /locus_tag="AC16CB_05150"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR3341887.1"
                     /translation="MNFSLRVVLFLLVASLLAGVVSGSNLYYRLAYLWIFLLAGSYLM
                     SRLALRNLSFQRTARSLRAQVGQFFDERFEIYNSSRLPRLWLEVRDETTLPGTRGSQV
                     LSMIGGRESRTYLVRTRLMQRGVFPLGQTILASGDLFGLFPVSHVYPAQDSLLVYPMM
                     VDVHAFPSPMGWLSGGEALRRRTHQITANASGVRDYAPGDPLNRIHWLSTARRNRLIV
                     KEFELDPLAEVWIFVDASRYVQSELPHQPLVLEEREVWRPSVKIPLPPSTEEYAVSIA
                     ASLARFFLQRKRAVGLVYAGRYFSVLSAERGGRQLGKILETLALLRADGKMPLQSLVD
                     AEVRSLPRGSTVILITPSTGNEVVLSAELLARRGMRPVAVLINSASFGGSAGSERIAS
                     NLEAMGIPVCRISYGDELSATLSDAAREQVWV"
     gene            complement(2506..3489)
                     /locus_tag="AC16CB_05155"
     CDS             complement(2506..3489)
                     /locus_tag="AC16CB_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3341888.1"
                     /translation="MPNVQAFSQGVIDNIEKVIVGKRATVEQAIISLLCQGHLLIEDV
                     PGVGKTMLARSLARSLGCSFSRIQFTPDMLPSDVTGVSIFNQVSREFEFRSGPVMAQI
                     VLADEINRATPKTQAALLEAMEERQVTVDGVTHSLPRPFMVLATQNPIEYEGTFPLPE
                     AQLDRFLMRIRLGYPGVEDEVLVMERQQYRHPLEILEQMVSAEELATMQETVKGVYVA
                     PSIKRYIVTLARHTREHPDVYLGASPRGSLALYRTSQALAAVRGREFVLPDDVKELVV
                     PTFAHRIILGPAARLRDLDDEQIVLEILENVPVPGGDLGESRVFDGSEVRA"
     gene            complement(3508..4623)
                     /locus_tag="AC16CB_05160"
     CDS             complement(3508..4623)
                     /locus_tag="AC16CB_05160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF046093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AZOBR_p60025 family cell surface glycopolymer
                     formation protein"
                     /protein_id="HIR3341889.1"
                     /translation="MRSRSFFDLRSWFLHPASITFIVLVAIATWWIADSGGDPLALAR
                     LGTKFSHGNPNGTEGYDGQFVYYIARDPSPEGVREYLDVPAYRYQRILLPLLARLVAF
                     GHIPALPWALIFLGILVQTAGTWVVTELMAGWGVSRWYALCYGLYAGFTLSIVLDLPE
                     PLAYGLIAGAFLAIERHKNKLAWLLFGLALFAKEVTGLFVAAALISAITNRRWVDVTG
                     ISLFALLPYGLFQLWLWEVFGQPGLGSGGAMATPFEVIPFMGLWRIGYYSLAYLAAML
                     VVFGPMIILPTLWGLVAGIKKWLSKDSEIFVIALILNALIMLFLPFSTYRETGGILRF
                     ACGLLLAVLIFSSRYRLWKVLNYSQFWFVLNVFLLKS"
BASE COUNT         1281 a         1266 c         1047 g         1088 t
ORIGIN      
        1 accacgatgt aatacacata cgaggcatta tatgcaatga cagttcgcga gttaggccag
       61 gttgcatcag gattgttggg cgcgtagtac cctccgtcct tgaggaaatg ataatctccc
      121 ccgatacccg cgtaggcttt cgaccagtcg tgtttaattg tttcagatgg gcaatcccta
      181 atttcttggg taatgcgcac ttcattaccc agagcaatat catcgctatt aatacggctc
      241 accatcgctg aacccacatc gccccacgca gctaacacga catgatcgaa tggaataggt
      301 gtagagccat tattgcgtcg gatttctcgg atgtaccctc gtacataact tggatcagaa
      361 accaataatc ccgggcgagt caattcaatc agtatttcca cgcgaggtgt ggcaccattt
      421 tccagcgttt tggttgtggc gtcatattgt ggggtataca ggatgagctg ttcatccgag
      481 cgtgccacat tgattgcgtc tatattagga tcatatgcta tacccaaaaa aaatatgtcg
      541 tttttttcgc caggatggcg atcaacacat ttgccaataa aagcttcccg attgagcttc
      601 cacacaaagc cagcatcgcc taccgtttca gaatatctct tggcatacca ccccgagtga
      661 atctgcccgc tccacggcac ccctggcagc tcttcgggat acccgaagaa atagccgttg
      721 atagctacca caacgcggtt gcgatttccc caggtttcgc cccaatagtt gatcgcctgg
      781 tcgtaacggg ctgccatgcc accaatggtt tccgtgcctc ccgaaagcct gccctgggcg
      841 atactgctct caatcgccac accaagatta cttcggtcca tacgcgccac aaaaatattt
      901 atatctcgag gataagtggg tttaccgaca tgatattttt gatactcaat gcctggagca
      961 acagccgacc aggcatctgg agattcaggc tcattctcgg gcgcgctgag taccatcccg
     1021 gtggggtaaa gaatgaatag gattaatata aaaagatggc ttattctgcg aaatctcatc
     1081 aatgatatcc cttaatgtct cagactttct ctatccaatt gataatatat tacccttttc
     1141 tcattattcg atcaagacat caatttttat tgctttttct gagcaatagt gtggatcatg
     1201 tccaacgaat atttatcaat gtcggttagc aaaatcacac ccacacttgt tcccgtgcag
     1261 catccgacag ggtagcagag agctcatctc cataggatat acggcaaacg ggaataccca
     1321 tggcttcaag gttggaggca atcctttcag aacctgctga tccgccaaag ctagccgagt
     1381 tgatgagtac agcaaccgga cgcatccctc gcctggctaa aagctctgcc gataaaacaa
     1441 cttcattacc tgttgagggg gtgataagaa tcactgtgct gcctctcgga aggctgcgca
     1501 cttctgcatc tacaagagat tggagcggca tcttcccgtc tgctctcaat aaggcgagcg
     1561 tctccaaaat tttacccaat tgtcgacctc cacgttctgc tgatagcacg ctaaaatagc
     1621 gccctgcata tactaagccg acagcccgct tgcgctgcaa gaaaaagcgc gccaacgagg
     1681 cggcgatgct cacagcatat tcttcagtgg agggtggtaa agggattttc acactgggtc
     1741 tccatacctc cctttcctcc aataccagtg gttgatgtgg gagttcgctt tgcacatagc
     1801 gggaggcatc cacaaaaatc cacacctccg ccagcggatc tagctcaaat tcttttacaa
     1861 tcaaccggtt tcggcgggct gtgctcaacc agtgaatgcg gttcaggggg tcaccaggtg
     1921 cataatcgcg cacaccggat gcgttagcag tgatctggtg tgtccttcga cgcaaggctt
     1981 caccacctga taaccatccc atcgggctgg gaaaagcatg cacatcgacc atcattggat
     2041 aaacaagaag cgaatcttgg gctgggtaca catggcttac tgggaaaagg ccgaataaat
     2101 cacccgaagc cagtatcgtc tgcccaagag ggaatacgcc gcgctgcatc aggcgcgtcc
     2161 ttaccaggta cgtgcggctt tcccgccctc caatcatgct gagcacctgg gatccacgcg
     2221 tgccaggcag ggttgtctcg tcgcgcacct ccagccataa gcgaggtagc cggctagaat
     2281 tatatatctc gaaacgctca tcaaagaatt gcccaacttg ggcacgcaaa gacctggcag
     2341 tccgttgaaa gctcagattt ctcaatgcca ggcgtgacat gaggtagctt cccgcgagca
     2401 aaaaaatcca tagatatgct aggcggtaat acaggttgga accactcaca acccccgcca
     2461 acaaactggc aacgagaagg aacaatacca cacgcagtga gaagttcatg ctctcacttc
     2521 actgccatca aaaactcttg attctcccaa atcgcctccc ggtacaggca cgttttcgag
     2581 gatctccaag acgatttgct cgtcatccag atcgcgtaga cgggcggcag gccccaggat
     2641 aattcgatga gcaaatgtcg gtactactaa ttccttgaca tcatcaggta gcacaaattc
     2701 gcgcccacgc accgcagcca gcgcttgaga tgtccgatac agagccaggc tgccgcgcgg
     2761 gctggcgcct agatacacat caggatgttc gcgggtatgc cgggctaaag taacaatata
     2821 acgcttgatc gaaggcgcta cgtacacccc ttttacggtc tcctgcatag tagccaactc
     2881 ttcagcggat accatttgct caagaatctc caatggatgg cggtattgct ggcgctccat
     2941 cactaatact tcgtcttcaa ctccaggata gcccaggcga attcgcatca agaatcggtc
     3001 taattgtgct tcagggagag gaaatgtccc ctcatattcg atcgggtttt gtgtagccag
     3061 caccatgaat gggcgtggca gggagtgcgt caccccatcg acggtaacct gtcgttcttc
     3121 catagcctct aatagagcag cctgggtctt gggtgtggcg cggttaattt catctgctag
     3181 cacaatttgc gccattactg gcccagagcg aaattcgaat tcccggctaa cctggttgaa
     3241 tatcgagacg ccggttacgt cgctgggaag catatcaggc gtaaactgta tccggctaaa
     3301 gctacatcct aaagagcgtg ccaggctacg cgcaagcatg gttttaccga cacctgggac
     3361 gtcttcaatc agcagatgtc cttgacatag caggctaatg attgcctgct ccacagttgc
     3421 acgcttccca acaatcactt tttcaatatt atcaattaca ccttggctga aagcttggac
     3481 gtttggcatc ttttactccc attagaacta tgactttagc agaaatacat tcaggacaaa
     3541 ccaaaactga ctatagttta acaccttcca gaggcgatac cgtgaagaaa atattaatac
     3601 agccagaagc agaccacaag caaatcgcaa aatcccacca gtctcacggt atgttgaaaa
     3661 cggcaagaac aacatgatca gggcgttcaa aattaaggca atcacaaaga tttcactatc
     3721 tttggagagc catttcttaa tacccgctac taaaccccaa agtgtcggta aaataatcat
     3781 gggtccaaag acaaccagca tggctgctaa atatgcaagg ctgtaatatc ctattcgcca
     3841 aagccccatg aacggaatca cctcaaacgg ggtcgccata gctccgcccg agcccaaacc
     3901 tggctgtcca aatacctccc acagccaaag ttggaataag ccataaggca ataaagcaaa
     3961 taggcttata ccagtcacat ccacccagcg gcgatttgtg atagcagata tcagcgcggc
     4021 tgccacaaaa agccctgtta cttcttttgc gaacagcgcc aaaccgaata gaagccaggc
     4081 tagcttgttt ttgtggcgct caattgccag aaatgcccca gctatcaacc cgtaagccag
     4141 aggctcgggc aaatccagca cgatggataa agtaaaacct gcatacaaac cataacataa
     4201 cgcataccag cggctcacac cccaaccagc cattaactcg gtcactaccc aggtgcctgc
     4261 tgtctgtacc aggatgccaa gaaaaatcaa tgcccaggga agtgctggta tatgtccaaa
     4321 cgccaccaac cgtgccagta acgggagcag tatgcgctgg tagcggtaag cagggacatc
     4381 taaatactcc cgcactcctt ctgggcttgg atcgcgggct atgtaataca cgaattgacc
     4441 gtcataacct tctgtgccat tcggattgcc gtgactgaac tttgttccca ggcgtgctaa
     4501 tgctaatgga tcaccgcccg aatcagctat ccaccaggtc gcaatcgcga ccaaaacaat
     4561 aaaagttata ctggcaggat gtaaaaacca gctacgaaga tcaaaaaagg atctacttct
     4621 catcgttttt tacgccatac cgcccaaatg atcaataaaa cgaaaaaact gatcaaaaca
     4681 cc
//
LOCUS       DBNGSX010000160         2106 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1535747, whole genome shotgun sequence.
ACCESSION   DBNGSX010000160 DBNGSX010000000
VERSION     DBNGSX010000160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1535747"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="AC16CB_05165"
     CDS             complement(<1..545)
                     /locus_tag="AC16CB_05165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR3341890.1"
                     /translation="MLRNLFIRLGLLNSILGITVLTILISILLTSGFSALIGDGPDLT
                     GLWTAIFVPAIITPIFSSIFLRMLFQFVSTESQLRELNRRDSLTGVYNRAYFIELAER
                     ELVRTLRYGETFSIAIIDIDDFKRVNDTYGHLAGDRALQVISEVSLHNLRQTDTIARF
                     GGDEFVVLFPYAGSQHVHESV"
     gene            complement(558..1070)
                     /locus_tag="AC16CB_05170"
     CDS             complement(558..1070)
                     /locus_tag="AC16CB_05170"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR3341891.1"
                     /translation="MMKNIYIGEVAPSPLGPIWVGLSERGLLAVEIQASRDAFCAHLL
                     RKFPGARVIIDQDRTAQAVQQIGEYLSGERRSFDLSIDWSGMTAFQEQALRVTLGIPY
                     GQVTTYGEIARQIGRPRAARAVGRAEATNPIPLVVPCHRVLGKDGGLHGFGAPGGVKT
                     KAWLLQLEGK"
     gene            complement(1077..1922)
                     /locus_tag="AC16CB_05175"
     CDS             complement(1077..1922)
                     /locus_tag="AC16CB_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3341892.1"
                     /translation="MMMWRSFRMASWLGWQIESNWADPFLFAVYSFAKPLAGAAILVV
                     MYSVITQGDFDSPIFPYMYLGNAFYIYVGQLMTGISWAVIDDREHYKTLKYIYAAPVN
                     FPVYLLGRGVARLLIASISVVVTILFGVFFLRLNIDLSAVNWPLFLVTLLIGVFMLSL
                     MGLILAGAMLLLVHHMWELGGAVAGALYLFSGAIFPLSVLPAFLRPIGYLMPITYWLE
                     LLRRSLVGSVAQAFPTLAGFNNSQLMGILLALTIAFAFISVGSFRWCENKARERGVID
                     VVTNY"
     gene            complement(1923..>2106)
                     /locus_tag="AC16CB_05180"
     CDS             complement(1923..>2106)
                     /locus_tag="AC16CB_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3341893.1"
                     /translation="LDAMRQLAFPSGESLGFLSVRVELGVLLVLCFLFLLGARWLLNY
                     IEKLAILEGRLTESRR"
BASE COUNT          629 a          598 c          469 g          410 t
ORIGIN      
        1 tcaacggact cgtgaacatg ctgacttccc gcatatggaa agagaaccac aaactcatcg
       61 cccccgaaac gtgcgatggt atctgtctgg cgcaagttat gtaagctgac ctcgctgatc
      121 acctgtaagg ctctatcacc agccagatgg ccataggtat cgttgacgcg cttaaaatca
      181 tcgatatcaa tgatagcgat cgaaaaagtt tctccatagc gcaaggtacg caccaactcc
      241 cgctcggcta attcaatgaa atacgctcga ttgtacacac cggtcaggct atctctcctg
      301 tttagttctc gcaattgact ttcggtgctg acaaattgaa acaacatacg caggaatatc
      361 gaactgaaga tcggtgtaat gatcgcaggg acgaaaatcg ctgtccagag tccggtcaga
      421 tcaggaccat ctccgattaa tgcactaaat ccacttgtca acagaatcga aatcaggatc
      481 gttagaacgg tgatccccaa tatcgagttt agcagaccca aacggatgaa cagattccgc
      541 aacatgcgat cttttgttta ctttccttca agttgaagta gccaggcttt agtcttcacc
      601 ccacctggtg cgccgaatcc atgcaagcca ccgtccttac ccagcacacg atgacatggt
      661 acaaccaagg gaatagggtt ggtagcctca gcgcggccta cagcacgagc agcgcgcggc
      721 ctgccaattt gacgggcaat ttcgccgtaa gttgtcacct gaccgtaggg aatgcccagc
      781 gtaacccgca gtgcctgctc ttgaaatgcg gtcatcccag accagtcaat agaaaggtca
      841 aaactccggc gttcccccga caagtattct ccaatctgct gaacagcctg cgcagtacga
      901 tcttgatcga ttataacccg ggcacccgga aatttgcgga gcaaatgcgc acaaaacgca
      961 tcccggctag cttgtatctc gacagccaac agcccacgct ccgatagccc tacccaaatg
     1021 ggacccaagg gggagggtgc tacttcacca atgtaaatat ttttcatcat tgttactcaa
     1081 taattcgtca ctacatctat taccccgcgc tcacgcgcct tattctcaca ccagcgaaat
     1141 gagccaacag aaataaatgc aaatgctatg gtcagcgcca acaagatacc cattaattga
     1201 gaattgttga acccggcaag cgtgggaaaa gcctgtgcca cagagcccac cagagatcgt
     1261 cgcagtaact ccagccaata agtaatcggc atcaaatagc caataggacg aaggaaagct
     1321 ggcaaaacac tcaggggaaa aatcgccccg ctgaacagat acagggcacc tgccactgcg
     1381 ccgccaagtt cccacatatg gtgcaccagc agcaacatcg ctcctgccag gattaaaccc
     1441 atcagcgata acatgaacac accaatgagc agagttacca ggaaaagcgg ccagttgaca
     1501 gctgacaaat caatattcag gcgcagaaag aatacaccga acaggatggt cactactact
     1561 gagatggagg cgatcagcaa gcgtgccaca ccccgcccca gcaagtagac agggaaattg
     1621 actggagcag catagatata cttcaaggtc ttataatgct cgcgatcatc aatcacagcc
     1681 caggaaatgc ccgtcatcag ctgcccgaca tagatataaa aagcatttcc taagtacata
     1741 tacggaaaaa tcggcgagtc aaaatccccc tgggtaatga cactatacat caccaccaga
     1801 atagccgccc ctgccaaggg tttggcgaac gaatatactg cgaacaggaa cggatctgcc
     1861 cagttcgatt cgatctgcca gcccaaccag gaagccatgc gaaacgagcg ccacatcatc
     1921 atttaccggc ggctctctgt cagccgcccc tcgagaatgg caagcttctc gatataattt
     1981 aacagccagc gcgcacccag tagaaaaagg aagcataaca ccagcagcac gcccagctca
     2041 acccgcacag agaggaaacc caagctttca ccggagggga aggcaagctg ccgcatggca
     2101 tccaac
//
LOCUS       DBNGSX010000161         2768 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1535768, whole genome shotgun sequence.
ACCESSION   DBNGSX010000161 DBNGSX010000000
VERSION     DBNGSX010000161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1535768"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..1616
                     /locus_tag="AC16CB_05185"
     CDS             27..1616
                     /locus_tag="AC16CB_05185"
                     /inference="COORDINATES: protein motif:HMM:NF037660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR3341894.1"
                     /translation="MAAKDFERAAGLIEEQVDAVWQRGEHAKILGWLNMLPKEQFSTR
                     PHLSIFHAWVQLEDGRVKAAELSLRAVEQAIKTNAANEPEKFVYPNSAHLKSRASVMH
                     AMLGFRTADAPSIIKFSTEALESLPEKDLSWRGIAAMALGDSHYIVGDMNGAYKALSE
                     AIRISKTAGNIYMTAYTSIKLAIILQYQGKLHQAQEICRELLTLMYASGLSRSALTGL
                     LHAQWGEILWDLNDVDNAFQYVSRGLELSKLENDVANLVWAHIVLVKVLYAKRDLTGA
                     MDTILKLEKIARKSHVPPWIVNRVAAWKARIWLTERNLVAIDHWVQGRQLNKDDELIF
                     AREVEYIVLARILIMKGELYDAVNLLERLIEKAEAGDRITRLIEMLLVLALAKNAQGD
                     IDNAVVTLLKSLSLAASGGHIRIFVDEGPSIEALLRRVKVENGKIKGHISTLLATFEE
                     KITGSPSRTQPLIEPLSERELEVLHLIAQGLTNQEISSRLYLSLNTVKVHTRNIYGKL
                     GVNNRTQAVARANGLGILLYA"
     gene            1845..2480
                     /locus_tag="AC16CB_05190"
     CDS             1845..2480
                     /locus_tag="AC16CB_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341895.1"
                     /translation="MKKQKIGLSLFWIGVIFALAFAAIGTRSLMHNLRNLTAEELDAT
                     IWAMGDPLFILWALSVTLGSVLAGIGAFAYVKTKQAFSWLTGIGVLGAVIVMVMVWSR
                     VYNATLFGIGGIIILVSFFAIVWVWIKKYAALESEEKTAGSYKLIGYIFWINASWFLC
                     GETAKMHLRAFEGQSAPSPIEIIVFLILGWVFVLIGEFKEMRLTRKKAVSL"
     gene            2569..>2768
                     /locus_tag="AC16CB_05195"
     CDS             2569..>2768
                     /locus_tag="AC16CB_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002169108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIR3341896.1"
                     /translation="MKAIVYTKYGPPEVLQLTKVRKPAPKENEVLIKVFATTVTKYDC
                     WMRSCTAPPGFGLLMRISSGRKP"
BASE COUNT          780 a          577 c          694 g          717 t
ORIGIN      
        1 tgggtaaaga ggcagtagat cacgcattgg cagctaaaga tttcgaacgg gcggcgggcc
       61 tgatcgagga gcaggtcgat gctgtttggc agcggggaga gcatgcaaaa atattgggat
      121 ggctgaacat gttgcccaag gaacagtttt ccaccaggcc acatctctct atttttcacg
      181 cctgggtaca attggaagat ggcagagtta aagcagcgga attgagctta cgggcagttg
      241 agcaagcaat taaaactaac gcagccaatg aaccagagaa atttgtctat ccaaactctg
      301 cacacctaaa aagcagggct tctgtgatgc acgccatgct gggttttcgc acagcagatg
      361 ctccaagcat catcaaattc tctaccgaag cacttgagtc tctacccgaa aaggatttgt
      421 cgtggcgcgg catcgctgcg atggcactag gtgattcgca ttatattgtt ggcgacatga
      481 atggggccta caaagccttg tctgaggcga tcagaattag taaaactgcg ggcaatatct
      541 acatgaccgc atacaccagt atcaaacttg ccataattct tcagtatcaa ggcaaattgc
      601 atcaagccca ggaaatatgt cgagagttat tgacgcttat gtacgcaagc ggattatcgc
      661 ggtcagcact aactggtttg ttacacgccc aatggggtga gattctatgg gacctgaatg
      721 atgttgataa cgctttccaa tatgtgagta ggggtcttga attgagcaaa ctcgaaaatg
      781 atgtggctaa tcttgtatgg gcacacattg tattggtgaa agttttatat gcaaagagag
      841 atttgaccgg ggcgatggat acaatcctga aattggaaaa aatcgcaaga aaatcccatg
      901 ttccgccctg gattgttaac cgggtagcag cctggaaggc acgcatttgg cttaccgaga
      961 gaaatcttgt tgccatagat cattgggttc aggggcgaca actgaacaaa gatgatgaac
     1021 tcatatttgc gcgcgaggtt gagtatattg ttcttgcccg cattctcata atgaagggtg
     1081 aattgtatga tgcggttaat ctgcttgaac gactgattga aaaagctgaa gcaggtgacc
     1141 gcattacgcg actgatcgaa atgttactgg ttctggcatt ggctaaaaat gcacaaggag
     1201 acatagacaa tgctgtggtc accttgttga agagcctttc actcgccgca tcgggaggcc
     1261 acatccgtat ctttgtggat gaaggcccgt cgatagaggc attattaaga agggtcaaag
     1321 tcgaaaatgg aaaaattaag ggacatatca gcacgctgct cgctaccttt gaagaaaaga
     1381 tcactggttc tccatctcgc acccaaccgc tgattgagcc gttaagcgag cgtgagctgg
     1441 aggtgcttca tctcatcgcc cagggactaa ccaatcagga aatctcttcc aggctttacc
     1501 tgtcactaaa cacagtcaag gttcacactc ggaatatcta cggaaaactc ggtgtcaaca
     1561 accgaacaca agctgttgcc agggcaaatg gattggggat tttgctctat gcttgattac
     1621 cagaaggtct gaattcggca ctggttacaa acaataacaa agaatactga cggggcacta
     1681 aacagtggcc cgtttttgtt tccttttcca ctttgaaatt aaccctctga ataacacttt
     1741 cggggtatga cacttataca cccggttgct atactgtttg tgcaagaatt cacttcttgg
     1801 aaaaagcaac tcctcaaatc aacttatcca atggagagct aaaaatgaag aaacaaaaaa
     1861 ttggtcttag cttattctgg attggcgtaa tctttgcctt agcattcgcc gctattggca
     1921 cgcggagtct tatgcacaac ctcaggaatc ttacggctga agaacttgac gcgaccattt
     1981 gggcgatggg cgacccattg ttcatactct gggctctatc tgtgactctg ggttcagtac
     2041 tggcgggcat tggcgccttt gcgtacgtga aaaccaagca agctttttcg tggctcactg
     2101 gtattggggt ccttggtgca gtcattgtta tggtcatggt ttggagccgt gtatataacg
     2161 caacgctgtt tggcattggc ggcatcatta tcttggtttc cttttttgca atcgtgtggg
     2221 tatggataaa aaaatatgcc gcgcttgaga gcgaagaaaa aaccgcaggg agctataagc
     2281 tcatcgggta tattttctgg ataaatgctt cctggttcct gtgtggagag actgcgaaaa
     2341 tgcacctaag ggcgtttgaa gggcagtctg cgcccagccc aatagagatc attgtttttc
     2401 taatcttggg ttgggtcttt gtgctgatag gtgaattcaa agaaatgcgt ctgacgagaa
     2461 aaaaagctgt aagcctctga gctggattgg agcatcactg attgactagg gcttatatga
     2521 tttcactaat ctgcaaaaag cagaaaaatg atgactaaaa aagacgaaat gaaagcgatt
     2581 gtatacacaa aatacgggcc tcctgaggtt cttcaactca caaaggttag aaaacctgct
     2641 cccaaggaga atgaggtact gatcaaagta tttgctacaa ctgtaaccaa atacgactgt
     2701 tggatgcgga gttgtaccgc gccgcccggt ttcgggctat tgatgcgaat atcatcaggg
     2761 agaaaacc
//
LOCUS       DBNGSX010000162         5002 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1743466, whole genome shotgun sequence.
ACCESSION   DBNGSX010000162 DBNGSX010000000
VERSION     DBNGSX010000162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5002)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1743466"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="AC16CB_05200"
     CDS             <1..416
                     /locus_tag="AC16CB_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3341897.1"
                     /translation="DFQAGDFWTQTSRARRSGESYDCVILDPPFFSVTPKGRVDLAGD
                     SARLINKVRPLVNQGGYLVAVNNALFISGADYLQTLEELCADGYLAIEELIPVPGDVT
                     GYPQTIRRSPPVDPAPFNHSTKIAILRILNRSSHE"
     gene            409..1458
                     /locus_tag="AC16CB_05205"
     CDS             409..1458
                     /locus_tag="AC16CB_05205"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR3341898.1"
                     /translation="MSKEIIFENARQVAFQDLPDPPLTARQVRVRTLYSGISAGTEMT
                     AYRGSNVYLFKHWDAKNRLFISAETPSSPYPLGGFGYEEVGEVIEIGEQVTCLKQGDI
                     VFGTWGHRTLHILEEEYASQRIKPADLEPILGIFSHIGPIALNGILDAQIHIGENVAV
                     FGLGVIGQIVAQLAKRSGARVFGVDPIETRLALAAQLGSIDVGLDPRQGSPAERIKAL
                     TDGLGADVSIEVSGSMPALHEAIRATSYSSRVVALGFYQGEANNLFLGEEFHHNRINL
                     VCSQISNVDPSLHFRWDRIRMIRTIMDFQVSGILNLRPLITHVIPFDEAAEAFRLIDT
                     SPEQVLEVVLDFSKV"
     gene            1468..2466
                     /locus_tag="AC16CB_05210"
     CDS             1468..2466
                     /locus_tag="AC16CB_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3341899.1"
                     /translation="MKRLRTAILGCGNFAKRHVQNLINLKDEVDLVAFCDHHEKNSRE
                     YAEKYTAGKAAFYTDHHVMLDQAELELMIVCLPPFAHSDEVESAAIHGVHLLVEKPIA
                     LTSEHAWRMVEAAEASKIKTQVGFMFRFGEAIEHMKALLNSGEAGQAGLMAARYFCNS
                     LHAHWWRKREKSGGQLVEQVIHMVDLLRYLLGEPSSVFSRQANLFHKDVPDYSIEDVS
                     TTIFSYDSGALGVIYATNGAIPNRWINDFRVVTRNVTAEFTNANNAVFTLTSKPDLPQ
                     QVIKSDRNVHLHELLDLLNAIRNGGETRTPLREGAKTLDLALAATTSAQNRREIFL"
     gene            complement(2498..4195)
                     /locus_tag="AC16CB_05215"
     CDS             complement(2498..4195)
                     /locus_tag="AC16CB_05215"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3341900.1"
                     /translation="MNRLRWQFLIVVLALVAIGVLLRFQQQPGIVQQPAPIQPTTGGV
                     YTEAIIGSFGRLNPVLDLFNPADRAATRLLFSGLVRFDDRGLPMGDLAESWGISEDGT
                     VYNFSIRPSAVWHDGEPVSSDDVVFTIEYLRSDELPLPEDLRAMWQQVEVRVLDEKTL
                     QFHLPEPFAPFLDYLSFGVLPRHLLVNVSPTELVDSDFNLKPVGSGPYRLDRLISEQG
                     QIKGVVFSAFEEYYMGRPFIDQVVFRYFPDANSAFTAYQAGEVLGISQVTMDILIEAL
                     NIEDLNFYSGRMPELTLIYFNLDETELPFFQDVSLRRALLMGLNRQRLVDQNLGGQAI
                     VADGPVFPGTWAYYEGIEHLDYDPNAALAAIKAAGYTIPAEGGSVREKEGVRFSFEMV
                     YPDNALHAGLVEMIRSDWAKLGVEVNPKPVTYDELVDNYLETRDYQAALVDLNLSRYP
                     DPDPYPFWDQAQATGGQNYGKWNDRRASEFLELARVSSDLDERAKAYRNFQVRFTTEM
                     PALPLFHPVYTYAVDAQVQGVRMGPLYDPSDRFATLTTWFLIAKRPVATPETATPAP"
     gene            complement(4197..4421)
                     /gene="secG"
                     /locus_tag="AC16CB_05220"
     CDS             complement(4197..4421)
                     /gene="secG"
                     /locus_tag="AC16CB_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR3341901.1"
                     /translation="MALYLDIALIITSIALIASVILQSKGAGLGGLTGSDTGGIFTAR
                     RGIEKVLFRITIGLSLLFFILAIATVIVKG"
     gene            4653..>5002
                     /locus_tag="AC16CB_05225"
     CDS             4653..>5002
                     /locus_tag="AC16CB_05225"
                     /inference="COORDINATES: protein motif:HMM:NF014826.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR3341902.1"
                     /translation="MHEIIVNLHMHTPYSDGHYTHSEIARAALETGLEAVIVTDHNVL
                     VNGVEDYFQEGSRKVLLLVGEEVHDQAREPQKDHLLVFGANRELATLAHNPQLLIDGV
                     QNANGISFIAHPVDP"
BASE COUNT         1292 a         1340 c         1244 g         1126 t
ORIGIN      
        1 cagatttcca ggctggtgat ttctggacgc aaaccagccg tgctcgccgt tcgggggaga
       61 gttacgactg cgtcattctt gacccccctt tcttctcggt gacacccaaa gggcgagtgg
      121 acttagctgg cgacagcgcg cggctgatca acaaagttcg tcccctggtc aaccagggtg
      181 gttatctggt ggcggtcaac aatgccttat ttatcagtgg ggctgactac ttgcaaactt
      241 tggaagagct atgcgcggat ggctacctcg ctattgagga gttgatcccg gtgccgggtg
      301 acgtgaccgg ctacccccag actatccgcc gctccccacc agttgatccg gctcctttca
      361 accacagcac aaaaattgcc atactacgta ttctcaatag gtcatcccat gagtaaagag
      421 attatttttg aaaatgcgcg ccaggtcgct ttccaagatt tgccagaccc cccgttgacc
      481 gccagacagg ttcgcgtgcg gacgctttac tcggggatca gcgctggcac cgaaatgaca
      541 gcataccgtg gtagcaatgt ttaccttttt aaacattggg atgcaaaaaa ccgcttgttc
      601 atctccgccg aaaccccttc ctcaccttac ccactaggtg gatttggcta tgaggaggtt
      661 ggcgaagtaa tcgaaattgg cgaacaggtc acctgcctca agcagggcga tatcgtattt
      721 ggcacctggg gacaccgcac cctgcatatt ctcgaagagg agtatgcttc ccagcggatc
      781 aagccagctg atctcgagcc aatcttgggc atcttctcgc acattggacc gattgcactg
      841 aatgggatat tagatgctca aattcatatc ggagagaatg tcgccgtctt tggcctgggt
      901 gttatcgggc agatagtcgc acaactcgca aaacgatcag gggctcgggt ctttggagtg
      961 gatccaattg agacccgctt ggctctggct gcgcagcttg ggtcgataga tgttggcctg
     1021 gacccgcgcc aaggttctcc agccgagcga ataaaagcct tgacagacgg gcttggcgca
     1081 gatgtatcca tcgaggtctc tggaagcatg ccagcactgc atgaagcaat ccgcgccaca
     1141 agctattcat cgcgtgtggt agctttgggt ttttaccaag gcgaggccaa caacctcttt
     1201 ttgggtgaag aattccacca caaccggatc aacctggtct gctcacaaat ttcaaacgtg
     1261 gatccatctt tacatttccg ctgggatcgc atccgaatga tccgcaccat tatggacttc
     1321 caggttagtg gaatactcaa tttgcgaccc ctcatcacgc atgtcatacc gtttgatgag
     1381 gccgctgaag cattcagact aatcgacaca tctccagaac aagtgctaga agttgttctc
     1441 gacttttcta aagtataggg gatcgagatg aaacgactac gaacagcgat cttgggttgc
     1501 ggcaattttg ccaaacgcca cgtgcaaaat ctaatcaact tgaaggatga agtagatctg
     1561 gttgcatttt gcgaccatca tgagaagaac tcccgtgaat acgcggaaaa atacaccgca
     1621 ggcaaagcag ccttctacac cgaccaccac gtaatgctcg accaggctga attggagttg
     1681 atgatcgtct gcttgccacc attcgcgcac agcgacgaag ttgaatctgc cgccatacac
     1741 ggtgtgcact tgttggttga aaagccgatt gcgctaactt cagaacacgc ctggcgtatg
     1801 gtggaagccg ccgaggcgtc gaagatcaag acacaagtag gttttatgtt ccggtttgga
     1861 gaagcgattg agcatatgaa agccctgctc aactccggcg aagctggtca agcggggctg
     1921 atggctgctc gatatttctg caattctctt catgcccatt ggtggcggaa acgcgagaaa
     1981 tcgggcggtc aacttgtcga gcaagtcatt cacatggtcg atttgctgcg ctatttgctg
     2041 ggcgagccct cgagcgtctt cagccgccag gcaaatctat tccataagga cgtgccagat
     2101 tatagtatcg aagatgtgag cactacgatt ttcagttatg actctggagc tctcggagta
     2161 atctatgcca cgaacggcgc cataccaaat cgctggatca atgatttccg cgtggtaacc
     2221 cgcaatgtaa ctgcagaatt tacaaatgcc aacaacgccg tcttcacgct cacatccaaa
     2281 ccagatcttc cacagcaggt tatcaaaagc gacaggaatg tgcatctaca cgagctgctg
     2341 gatttactga atgcgatccg taacggtggt gaaacccgaa ctccgctccg tgagggagcg
     2401 aaaacactcg acctggcact tgcagctact acatctgccc agaatcgacg agaaatattt
     2461 ttatagcaag aaggcgggag atcttataac aaaattttta gggagcgggt gtagccgtct
     2521 ctggagtagc cacaggccgc tttgcgatca ggaaccatgt tgtgagcgta gcaaagcggt
     2581 cgctagggtc atataacgga cccatacgca ccccctgaac ctgtgcgtcc acagcataag
     2641 tgtatacagg gtgaaacagc ggaagagccg gcatttccgt cgtgaagcgc acctgaaaat
     2701 tacggtaggc tttggcacgc tcgtcgagat ctgagcttac gcgggccagc tctagaaact
     2761 cgctcgcccg gcgatcattc cacttaccat aattctgacc acctgttgcc tgagcttgat
     2821 cccaaaatgg gtaagggtcg gggtcaggat aacgggacag attaaggtcc accagtgcag
     2881 cctggtaatc gcgcgtctcc agatagttat ccaccagctc gtcataagtt accggtttgg
     2941 gatttacttc aacgccgagc ttcgcccaat cactgcggat catctcaact aacccagcat
     3001 gcagggcgtt gtctggatag accatctcaa acgagaacct tacgccttcc ttctctcgca
     3061 ccgagccacc ctcggctggg atggtataac ccgcggcttt gatagctgcc agcgctgcgt
     3121 tgggatcata atccaggtgt tcgatacctt cataataagc ccaggtacct gggaagaccg
     3181 gcccatcagc gacaatcgcc tgaccaccca ggttttggtc caccaagcgc tggcggttca
     3241 gacccatcaa taatgcgcgg cgtaaggata catcctggaa gaagggcagc tcagtttcat
     3301 ccagattgaa atagatcaaa gtcaattccg gcatgcgccc agaatagaag ttcaaatcct
     3361 cgatatttag cgcctcaatc aggatatcca tcgtaacctg gctgattcca agcacttcac
     3421 cagcctggta agctgtaaaa gccgaatttg cgtctgggaa ataacggaaa accacctgat
     3481 cgatgaatgg ccggcccatg taatactctt caaaagcaga aaaaacgact cctttgattt
     3541 gaccctgttc ggagatcaag cgatccaagc ggtatggccc actgcccacc ggtttgagat
     3601 tgaagtcgga atcaactagc tcagtaggtg acacattcac caggaggtgc cttggcaaca
     3661 ccccaaagga aaggtaatct aagaaaggcg caaacggctc aggcaggtga aactggagag
     3721 tcttttcatc cagcaccctg acttccacct gctgccacat ggcgcgcaaa tcttctggaa
     3781 ggggcaattc gtcgctgcgc aggtactcaa tcgtgaatac tacgtcatcg ctggaaacag
     3841 gttccccatc gtgccagacc gcggaaggac gtatggagaa attgtaaact gtcccatctt
     3901 cagagattcc ccacgattca gctaaatcac ccatgggcaa accgcggtcg tcaaaccgca
     3961 ccagaccgct aaataacaag cgagtcgcgg ctcgatcggc agggttaaaa aggtcgagga
     4021 ccgggtttag acgaccaaaa gaaccgatga tggcttcggt ataaacgccc cctgtcgtcg
     4081 gttggatcgg ggcaggctgc tggacaatgc ccggctgctg ctgaaatcgc agcaacacac
     4141 caatagccac cagcgccagc accacaatga ggaattgcca gcgcagccta ttcaaactag
     4201 cctttgacga tcaccgtcgc tatggccagg atgaaaaaca ataaactcaa accgatagta
     4261 atacggaaga gaaccttttc aatcccgcgc cgcgcggtaa aaatgccccc tgtatctgag
     4321 ccggttaatc cacctaatcc tgcgcccttg ctctgcaaga tgacactagc gattagagca
     4381 atagacgtta taatcaatgc gatatccagg tataatgcca caatgtcctc cggacaaatt
     4441 tacatactaa atgggccttt gggtccgttg tttttaagcc tgcgtttgtg catggtgcaa
     4501 cccaaagagc ctattgtttt caagcaggcg gattatacct tcgcgcaggc gtttcgtcaa
     4561 atcaagaatt atgtctctaa ctaagcaact tcacacaaaa acgccgcatc cgtcgtctaa
     4621 tatgagccat ctttttccac aaccgagcag ccatgcacga aatcatcgtt aacctgcaca
     4681 tgcatacacc ctactccgac gggcactata cgcactccga gattgcacgg gcagccctag
     4741 aaacaggatt ggaggctgtc atagtcaccg accacaacgt acttgtaaat ggggtagaag
     4801 actatttcca ggaaggcagc cggaaggtcc tgttactggt cggcgaagag gttcacgatc
     4861 aagcacgcga gccgcaaaag gatcatttgc tggttttcgg agccaaccgg gagctggcta
     4921 cactggcgca caacccgcag ctccttatcg atggtgtaca gaatgcaaac ggtatttcat
     4981 tcatcgccca tccggttgac cc
//
LOCUS       DBNGSX010000163         6795 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1813013, whole genome shotgun sequence.
ACCESSION   DBNGSX010000163 DBNGSX010000000
VERSION     DBNGSX010000163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6795)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1813013"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="AC16CB_05230"
     CDS             <1..902
                     /locus_tag="AC16CB_05230"
                     /EC_number="1.17.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HIR3341903.1"
                     /translation="LYILGEDPVMSDPDSNHIRHCLESCDFIVLQEIFPSETAVFADV
                     LLPGASFAEKTGTFTNTERRIQMVRSAIDPLGDARFDWQITSDLAKRILGSSSRKVGN
                     GTHASWDFTDTSEIMDEIAALTPSYAGISQARLERGERLQWPVKGPDHPGTPILHVGQ
                     FARGRGKFMPIDHVPPAEMPDDDYPMLLSTGRVLYHWHGGEMTRRAEGLMEVYGQSLI
                     EVNPEDATRLGLNGNQRVRVTSRRGSIEAQAWVTERVPPGMVYANFHFPDASANELTI
                     AALDPVAKIPEYKVCAVKVESVA"
     gene            918..1409
                     /locus_tag="AC16CB_05235"
     CDS             918..1409
                     /locus_tag="AC16CB_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HIR3341904.1"
                     /translation="MDVRKIYEYALAREHEGKRFFETNAARMSHAAAVGAFKQLAAEE
                     QKHIDFITLQIAAFDRGLYSDMSVKGELEKGGLFSQRASLESIDQTISEAMVPDLPVL
                     RMAYLIERDFAEFYEMAAQKMEGEPKQVLSMLAQWERGHERYFKEWHDKAFEEYAKMP
                     WGG"
     gene            1507..2691
                     /locus_tag="AC16CB_05240"
     CDS             1507..2691
                     /locus_tag="AC16CB_05240"
                     /inference="COORDINATES: protein motif:HMM:NF027484.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HIR3341905.1"
                     /translation="MNPRIYRPIIALPMAFLLMIACNFFSPTAPPGEFDQGQLYTQAA
                     ETLSVQLTQVALFATATDLSKPTQTPTQIVLPSNTPLPTATNTPVPTATQFPTATRVP
                     PTSTPRPCLAARFVRDVTVPDGTKYAGGTSFTKTWELENVGSCTWTDDFELVFVKGDR
                     MSGDDNIPIDERVEPGETVRVSVDLLAPFTKGEYRGDWMLADDDGEEFGLGAGANKSF
                     WVSIRVTEVKEGVVLSFVDSYCSADWESSAGDLPCPGKGTEPSGFVVRLEEPDQENRK
                     ENEPALWTNPEMEDDGWITGTYPAIKIKDGDRFIADIGCLDDSEDCDVIFRLNYKIGS
                     GPVKNLGEWHEVFDNLITRVDIDLSDLDGENVKFILTVTVNDDYDEDAAFWLQPHIQR
                     ED"
     gene            2895..3611
                     /locus_tag="AC16CB_05245"
     CDS             2895..3611
                     /locus_tag="AC16CB_05245"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3341906.1"
                     /translation="MGALLIHGLTATTAEVRPLGQILHQRLGYTISGPLLPGHNTRPD
                     DLNRFRWQDWLATVDEAYKNLSQRCQTIIVGGESTGALLALYLASQYPEIACILIYSP
                     ALKLIVSRFDLIRLYLIAPFVSSMPKGSLDSPNEWQGYPVNPLKGAIQLLKFQEVVKK
                     RLADIHQPILIVQGRLDTTVHPTAPQMIYDTVNSRVKELHWMENSSHVVIIDKELDSI
                     AEITIRFIEKSLSQPAKEHG"
     gene            3674..4258
                     /locus_tag="AC16CB_05250"
     CDS             3674..4258
                     /locus_tag="AC16CB_05250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR3341907.1"
                     /translation="MEKNLLALVMSYLIGSIPTALIVSRVVAGVDIRQIGDGNMGARN
                     VTRSLGWGPGIVVAAFDFSKGGLGVLVARQVGYSLNWQLACGVSAVLGHDFPIFAGFR
                     GGQGMATTLGTLFVFMPYQTFLGLITFAAAYYMWRNFDFSAATGLGLLAVFAWISKQP
                     FILVAYTVLLFISIPAKKILDWPRRRRLAQGHKQ"
     gene            4336..5847
                     /gene="glpK"
                     /locus_tag="AC16CB_05255"
     CDS             4336..5847
                     /gene="glpK"
                     /locus_tag="AC16CB_05255"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015909396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HIR3341908.1"
                     /translation="MPKYIAAIDQGTTSTRCMLFDHSGGVVSVSQKEHRQIYPQPGWV
                     EHDPLEIWERTQDVIRKAVATSDAQQGDIAAIGVTNQRETSIVWDRRSGSPYYNAIVW
                     QDTRTKQICDALSSQGGQDRFRAKVGLPLATYFSGPKIRWILDHVQGVRAAAERGDAL
                     FGNIDSWIIWWLTGGPAHGAHVTDVSNASRTMLMNLETLDWDDEILSEMEIPRAMLPR
                     IVPSSDPRTWGNTATDSPFGNGIPVCGDLGDQQAALVGQTCFSVGEAKNTYGTGCFML
                     LNTGSDIVQSTSGLLTTLAYKMGDKPATYALEGSIAITGALVQWLRDNLGLIEKSTQI
                     EELARSVEDNGGIYFVPAFSGLFAPYWRSDARGAIVGMTRYVNRGHFARAALEATAYQ
                     TREVLDAMEKDSNVKLKSLKVDGGMVFNELLMQFQADILDVPVVRPKVAETTALGAAY
                     AAGLAVGFWENQEDLRQNWQVDKTWDPQMTDKARQELYHGWLKAVERTFGWID"
     gene            5844..>6795
                     /locus_tag="AC16CB_05260"
     CDS             5844..>6795
                     /locus_tag="AC16CB_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3341909.1"
                     /translation="MNKKSLDTEILVIGGGATGTGVARDLAMRGFKTILVEKADLTHG
                     TTGRYHGLLHSGGRYVVKDPQAAKECIEENRILRKIMPQCIEDTGGYFVVTPWDDPNY
                     APLFVEGCKSAGIPVEELSIAQMLRSEPLLNPEIIRCFRVPDASADSFLGADLNADSA
                     QAHGAQILTYHKVVRLLTEESRPGHTPKKVAGAVCQDLVKDQEVVIHADMVVNASGAW
                     AGKIAETASIPLTIIGGKGTMIAVNHRIVQTVINRCKMPSDGDILVPAHTVCVIGTTD
                     VKISDPDHFAIEPWEVHLCLEEGDKLVPGFKNMRMLRAWAG"
BASE COUNT         1756 a         1705 c         1730 g         1604 t
ORIGIN      
        1 ccctttacat actgggtgaa gatccggtga tgagcgaccc agatagcaac catatccgcc
       61 attgtctcga atcttgtgat tttatcgtcc tccaagaaat attcccatcc gagaccgcgg
      121 tttttgctga tgtgcttcta cccggcgcaa gctttgccga gaagaccggc acattcacca
      181 ataccgagcg ccgaatccag atggttcgca gtgcgattga cccattgggt gatgcgcgtt
      241 tcgactggca aatcaccagc gatctagcta agcgcattct gggaagtagc agtcgcaaag
      301 taggcaatgg gacacacgca tcttgggact ttacagatac atccgagatt atggatgaaa
      361 ttgccgccct tacgccaagc tacgcaggca tcagccaggc gcgccttgag cgtggtgaac
      421 gactgcaatg gccggttaaa ggacctgatc acccgggaac gccgatccta catgttggtc
      481 agtttgcccg aggtcgaggg aaattcatgc ccatcgatca cgtacccccg gctgagatgc
      541 cagacgatga ttatccgatg ttactcagca ctggccgcgt gctatatcac tggcatggag
      601 gagagatgac ccgccgcgcc gaggggctca tggaagtcta tggacaatcc ttgatcgaag
      661 tcaaccctga agacgcaacg cgccttggac taaatggcaa ccaacgtgtg cgagtcactt
      721 cccgcagggg gagcatcgag gctcaagctt gggtgactga acgtgtacca ccaggcatgg
      781 tttatgcgaa ttttcatttc cccgatgcat cagcaaacga attaactatc gcagctcttg
      841 atcccgtagc aaaaattccg gaatataaag tctgtgcagt caaagtagaa tccgttgcat
      901 aggagaggta tacagccatg gatgtccgaa aaatctacga atacgccctt gctcgtgagc
      961 atgaaggaaa acgcttcttt gagaccaacg cggcccgtat gagccatgcg gcagcggtcg
     1021 gtgcgttcaa gcaattagct gctgaggaac aaaaacatat cgacttcatc actctacaga
     1081 tagctgcctt cgaccgaggg ttgtattctg atatgtccgt gaagggtgag ctggagaaag
     1141 gcggattatt ctcgcaacgc gcttcccttg aatcaatcga tcagactatt tctgaggcaa
     1201 tggtccccga cctgcccgtc ctccgcatgg catatcttat cgagcgcgat tttgccgaat
     1261 tttacgagat ggccgcacaa aaaatggagg gcgaaccgaa gcaggtcttg agcatgttag
     1321 cccagtggga acgcggtcac gagcggtact tcaaagaatg gcatgataaa gcctttgaag
     1381 agtatgctaa aatgccctgg ggtggttgac gcggaacaaa aaacaattgt atatcgtcag
     1441 tagcataggc cattagcagc acctaggcct catttatctt gcgtgatttc tgaggagcag
     1501 atatttatga acccgagaat ttaccgccca atcattgccc taccaatggc atttctatta
     1561 atgatcgcct gcaatttctt ttcgccaacc gcgcctcctg gtgaatttga tcaaggtcag
     1621 ttatacaccc aggcagctga aacattaagt gttcagctaa cgcaagtcgc cttgttcgct
     1681 acggcaactg atttatccaa gccgacacag acacctaccc aaatcgtgct gcccagtaac
     1741 acgcccttgc ctaccgcaac aaatacgcct gtgcctaccg ctacccaatt tccgacagcg
     1801 acacgtgtcc cgcccacgtc gacccctcgg ccttgtctgg ctgctaggtt tgtcagagac
     1861 gtcaccgtac cagatgggac gaaatatgcc ggaggtacgt ccttcaccaa gacctgggag
     1921 ctcgagaatg tcggatcttg cacatggacg gatgattttg aactggtctt tgtcaagggg
     1981 gatcgtatga gcggtgatga taatatccct atcgatgaaa gggtcgaacc cggcgagaca
     2041 gtcagagtat cagttgatct gttagccccc ttcacaaaag gtgaatatcg tggtgattgg
     2101 atgctagcag atgatgatgg cgaagaattt ggcttaggtg ccggagccaa taaatctttc
     2161 tgggtaagca tccgtgttac agaagtgaaa gaaggagtag tacttagctt tgtagattca
     2221 tactgctctg cagattggga aagcagtgcc ggtgatctac cttgcccagg taagggcacc
     2281 gaaccatctg gctttgtcgt tcgcttagaa gaaccagatc aagaaaatcg aaaagagaat
     2341 gagcccgccc tatggaccaa tccagagatg gaagacgacg gctggatcac tggcacttat
     2401 ccagccataa aaattaagga cggagatcgt tttattgccg atatcgggtg ccttgacgac
     2461 agtgaagatt gcgatgtcat attcaggctg aattacaaga tcggcagcgg accggtcaaa
     2521 aatttgggcg agtggcacga agtatttgac aacttaatca cccgcgtcga cattgaccta
     2581 agcgatctgg atggagaaaa tgtaaaattc attctcacag tgaccgtgaa tgacgattac
     2641 gatgaagatg ccgcattttg gctacaacct cacattcaga gagaagatta atactaacct
     2701 aacgagaaaa acggcctcgc ctctaatcag ggtgaggccg tttgtttacc tattagcccc
     2761 atttatttac tgtttgactt tcccatgtta agatataagc aatgaaccca gatccttctt
     2821 cggcagcagt atacaatccg ttcctccata atccacatct tgatgggtat acattcttct
     2881 gggaaggagg tcaagtgggt gcactgctca tccacggatt gaccgccacc actgctgaag
     2941 taagaccatt gggtcagata ttgcaccaac gccttggtta tacaatctca ggtcccctgt
     3001 tacccggcca caacacgcgt cccgatgacc tcaatcgatt ccgctggcag gattggcttg
     3061 ccaccgtgga tgaagcctat aaaaacctat cccagcgatg ccaaacgatt attgtcggtg
     3121 gcgaatcaac tggcgccctg ctggcgctat acctagcaag tcaataccca gaaattgcgt
     3181 gtattcttat ttattcccct gctctgaagc ttatcgttag ccgattcgac ctaatccgcc
     3241 tttacctgat cgcccccttt gtatcatcta tgccaaaagg tagtttagat agcccaaatg
     3301 aatggcaagg ctacccggtc aatcctctga agggtgcaat tcaattgcta aaatttcagg
     3361 aggtagtaaa gaagcgattg gcagatatcc accagccaat cctgatcgtt caaggtcgtc
     3421 tagatacaac agttcatccc actgccccac agatgatcta tgacactgta aattccagag
     3481 taaaagaact ccactggatg gaaaactctt cacatgtagt cattatagat aaggagctgg
     3541 acagtatcgc ggagattacg attcgtttta tcgaaaaatc cttgagccag ccagcaaaag
     3601 agcacggtta atcgcaccca ataaaacctg acctcctggt acattgagca tacagaacgt
     3661 ctctacgtca agtatggaaa agaacctgct cgccttagtg atgtcttacc tgattggctc
     3721 cattcccact gcgttgatcg tttcgcgtgt cgtggctggc gtggacattc gacagatagg
     3781 tgatggtaac atgggggcgc gcaatgttac gcgcagcctg ggttggggac caggaatcgt
     3841 tgttgcagcc ttcgatttca gtaaaggtgg attgggcgtg ctggtggcgc gacaagtggg
     3901 ttattcactg aattggcagc tcgcctgcgg tgtgagcgcc gtgctggggc atgatttccc
     3961 tatttttgcc ggatttcgag gaggtcaggg tatggctacc acgcttggga cgctctttgt
     4021 atttatgccg taccaaactt tcttaggatt gatcacgttc gcagctgctt attacatgtg
     4081 gcgtaacttc gactttagcg cagccacagg attaggcctc ttggcggtgt tcgcctggat
     4141 ttcaaagcag ccgtttattc ttgtagcata cacagtatta ctattcattt ccatccctgc
     4201 taagaaaata ttagactggc ctcgccgtcg gcgattggct caaggacata agcaataaat
     4261 ttctgaccct ggcagtagtc catgatttca tactattgac aaattccagc tcttcctgaa
     4321 aaaaggaata cccctatgcc gaaatacatt gctgcgatag accaagggac taccagcacc
     4381 cgctgcatgc ttttcgacca cagcggaggg gtagtcagcg tctcacaaaa agaacatcgc
     4441 cagatatacc ctcaaccagg ctgggttgaa catgacccgc ttgagatttg ggagagaacg
     4501 caagatgtta tccgaaaagc agtcgccacg tccgatgccc aacaagggga tattgctgca
     4561 attggcgtca ccaaccagcg ggaaacctcg attgtctggg acaggcgctc tggaagcccc
     4621 tattacaatg ccatcgtctg gcaggacacg cgcacaaagc agatctgcga cgctttgtct
     4681 tcgcaaggag ggcaggatcg ttttagagcc aaagtcggct tgccactggc tacttatttc
     4741 tctggcccaa aaatcaggtg gattcttgac catgtacaag gtgttcgtgc tgccgccgag
     4801 cgtggcgacg ccctgtttgg caacatcgat agctggatta tttggtggct aactggcggc
     4861 ccggctcatg gcgctcatgt tactgatgtc agcaatgcca gtcgcacgat gttgatgaac
     4921 ctagagactt tggactggga tgacgagatt ttaagcgaaa tggaaatccc gcgcgccatg
     4981 ctgcccagga ttgtgccgtc aagcgatcca cgtacatggg gaaatacagc tacagacagc
     5041 ccctttggca acggcatacc cgtttgcgga gacttaggag accagcaagc cgccctggtg
     5101 ggtcagactt gtttcagtgt gggcgaagca aaaaatacat atggcacagg ctgcttcatg
     5161 cttctaaaca caggctcaga cattgtacaa tccacgagtg gattattaac caccctggct
     5221 tataaaatgg gcgacaaacc tgccacctat gctcttgaag gttccatcgc catcactggg
     5281 gctctggttc aatggctccg cgacaacctc ggtctaatcg aaaaatccac ccagattgaa
     5341 gagctagcgc gcagcgttga agataatggc ggtatttact tcgtaccagc attctctgga
     5401 ttgttcgccc catattggcg ctcggatgcc cgcggggcta tagtcggtat gacacgctac
     5461 gtgaataggg gacacttcgc acgcgcagcc ctggaagcca ccgcctatca gacacgcgaa
     5521 gtgttggatg cgatggaaaa ggattctaac gtcaagttga aatctctcaa ggtcgatggc
     5581 ggcatggtat tcaacgaatt gctgatgcag ttccaggctg atatattaga tgtcccagtt
     5641 gttcgcccca aagtggcaga gaccaccgca ttgggcgcgg cttatgctgc tggcttagcc
     5701 gtcggatttt gggagaatca ggaagacctg cggcaaaatt ggcaggtgga taagacctgg
     5761 gatccacaaa tgaccgataa agctcgccaa gaactgtacc acggctggct gaaagcagtc
     5821 gagagaacgt ttgggtggat cgattgaaca agaaaagcct tgacaccgaa atactcgtca
     5881 taggtggcgg cgcaaccggg accggcgtcg cccgcgatct ggctatgcgg ggtttcaaga
     5941 cgatattggt tgaaaaagca gacctgacgc acggtacaac tgggcgttac catggactac
     6001 tgcatagcgg cgggcgctat gttgtcaaag accctcaagc cgccaaagaa tgcatcgagg
     6061 aaaatcgtat tctgcgcaag attatgcctc agtgtatcga agatacaggc ggttatttcg
     6121 tcgtcacgcc ttgggacgat cccaactacg cccctctctt cgtggagggt tgcaagagtg
     6181 ctggtatacc agtcgaagag ctctcgatag cccaaatgtt gcgttcagaa cccctattaa
     6241 accctgaaat tatccgctgc tttcgtgtcc cagatgcatc agccgattcg tttctgggag
     6301 cagacctgaa cgctgactcc gcacaagccc atggggcaca aatattgacc tatcataaag
     6361 tggttcgttt gctcacagag gaaagtcgcc caggtcatac ccccaaaaaa gtcgctggcg
     6421 cagtttgtca agacctggtt aaggatcaag aagtcgtcat tcacgctgac atggtggtaa
     6481 atgcctccgg tgcttgggca ggaaaaatag ctgaaacagc gagtatcccc ctcacgatca
     6541 tcgggggcaa aggcaccatg attgcggtga atcaccgcat tgtacagact gtgatcaacc
     6601 gctgcaaaat gccctccgat ggcgacattt tggtacctgc tcatacggtc tgcgtgattg
     6661 gcaccacaga tgtaaaaatc tccgatccag accactttgc catagagcca tgggaagtgc
     6721 atctctgcct ggaagaaggt gacaaactgg tacccggctt taagaatatg cgcatgctgc
     6781 gtgcctgggc ggggg
//
LOCUS       DBNGSX010000164         2634 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1397344, whole genome shotgun sequence.
ACCESSION   DBNGSX010000164 DBNGSX010000000
VERSION     DBNGSX010000164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1397344"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(315..731)
                     /locus_tag="AC16CB_05265"
     CDS             complement(315..731)
                     /locus_tag="AC16CB_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR3341910.1"
                     /translation="MSDDVIRQILTTSDVIATVGLSANPAKTSHGVAQYLIKQGYDVI
                     PVNPGVDEIFGRKAYPDLISIPRKIDVVQIFRPSSDVPPIVDQAIQIGAKVVWMQEGI
                     TNPEAAETARAAGLQVVMDRCMRQEHIRLVGHIMDM"
     gene            complement(728..2128)
                     /locus_tag="AC16CB_05270"
     CDS             complement(728..2128)
                     /locus_tag="AC16CB_05270"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3341911.1"
                     /translation="MNAELLQKAPLLASLSPEDLDWLATLSINVVVQAGETLIREGDE
                     GDSAYLILDGELEVSKRSGDRDVMLSVRRSGEVIGEMALLTQAPRMATVRALQETHLC
                     KIPAEAFEQLLRTHPSATLVILRTVTTRLKNTEALLHQREKMASLGTMAAGLAHELNN
                     PAAAAQRSVAQLQEALVNWQKLNFSLQQSASDERTSMLIDQLHAELLRRSAVPASLDP
                     LARLDQEQSLQEWLEERGMDDAWQLTQPLVLAGWSSDELNSFTGSIPSEQFIQLIRWL
                     AAGCLVYSLLDEVHQSTSRISEIVKGVKNYTYLDQAPIQEVDVHEGLENTLLILRHKL
                     KTGVNVIRQYAQNLPHIEAYASELNQVWTNIIDNGIDAMQGQGELILRTYAKNDHVVV
                     EIQDSGPGIPPDIQPRIFDPFFTTKPPGSGTGLGLNIAYGIVVNKHCGEIHLSSKPGA
                     TCFQVILPVRLGKDKQ"
     gene            complement(2137..>2634)
                     /locus_tag="AC16CB_05275"
     CDS             complement(2137..>2634)
                     /locus_tag="AC16CB_05275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3341912.1"
                     /translation="ALYLSRFARKVNLIVRGELSAAQYLADELRQNEKIETYLNTDLI
                     DVRGEGKLEEIVVKNNRTDELQTLKGAAMFVFIGVRPQSQFVADLVARAEKGYVLTGP
                     DLLVEGKRPANWPLDRDPYLLETSVPGIFAAGDVRYGTTHRVSAATSEGGLAVALIRQ
                     YLKTI"
BASE COUNT          595 a          753 c          663 g          623 t
ORIGIN      
        1 acagtatcga tcgtggcgaa ggaaagcatg cttagctttt catcgcgccc ttgctctgcc
       61 tgccgaaagc gctgcgaggc ttcatcggct ttgcgcaagg ctttgtcttg aatagattcc
      121 atgacaatct cctagggtaa ataatagaat atgctactta catctacgcc gcacccctga
      181 atagtctgga ggatgtgatt gtatccatag cgttctataa tctctcctcc cggggaaggg
      241 gagacagcct gcccaccgga acggcagcgc acgcagggga tgaggaatat aatatccaaa
      301 tggtctctat accatcacat atccataata tggccaacca ggcgaatgtg ttcctggcgc
      361 atacagcgat ccatcaccac ctgaagaccg gcggcgcgcg ccgtctcagc ggcttctgga
      421 ttggttatac cttcttgcat ccacacaact ttcgctccaa tttgaatcgc ctggtcgaca
      481 atcggaggca catcgctcga cggcctgaaa atctgcacca cgtctatttt acgcgggatt
      541 gaaatcaggt cagggtaggc cttgcgacca aatatctcgt ctaccccggg gttgaccggt
      601 atcacatcat acccttgttt gattaagtat tgggcaaccc catggctggt cttagccgga
      661 ttggccgaca atcccacagt tgcgattaca tcgctggttg tcaatatttg tctgataacg
      721 tcatcactca ttgtttatcc tttccgagac gtaccggtaa tattacctgg aagcaggtcg
      781 caccgggctt ggatgacagg tgaatctcac cgcaatgctt gttgaccaca ataccgtaag
      841 cgatgttcaa ccccagtccg gtcccgctgc caggaggttt tgtggtaaag aacgggtcaa
      901 aaatgcgcgg ctgaatatcg gggggaatgc ccggccccga atcctgtatc tcgaccacca
      961 catgatcgtt tttcgcatag gttctcagaa tcagctcacc ctgcccctgc atggcatcga
     1021 taccgttatc gatgatgttc gtccacactt ggttaagttc gctggcatat gcctcgatat
     1081 gcggtaaatt ctgcgcatac tgccggatga catttacccc cgtctttagc ttatggcgga
     1141 ggatcaacag tgtgttctcc agaccttcgt ggacatccac ctcctggatc ggcgcctggt
     1201 ctagatacgt ataatttttg acccccttga cgatctccga gatgcgactg gtgctttggt
     1261 gcacttcatc taacaggctg tacaccaggc agccggcagc cagccagcgg atgagctgga
     1321 taaattgttc agagggaatg gaaccagtaa aggaattcag ctcatcagaa gaccatccag
     1381 ccagaacaag gggctgcgtc aattgccagg catcatccat accgcgctct tccagccact
     1441 cttgcaggga ttgttcctgg tcgagccgcg ccagtgggtc aaggctggct gggacagcac
     1501 tgcggcgcaa tagctcggca tgcaattgat cgatgagcat agaggtgcgt tcatcactcg
     1561 cactctgctg caagctaaag ttcagcttct gccaatttac gagcgcctct tgtaattggg
     1621 ctacactgcg ctgagcagcc gcagcaggat tgttcagctc atgggcaaga ccagccgcca
     1681 tcgttcccaa tgaggccatt ttctctcgct ggtgcagcaa ggcctcggta ttcttcagtc
     1741 gggtggtgac tgtacgcaat attaccagcg tagctgaagg atgagtcctg agcaactgtt
     1801 caaacgcctc agccgggatc ttgcacaagt gggtttcctg caaggcgcgc acagttgcca
     1861 tgcggggagc ctgcgtgagc aaagccatct ccccaatcac ctctccagac cggcgaaccg
     1921 ataacatgac atcccggtct ccagagcgtt tgctcacctc gagctctcca tccaggatga
     1981 gataagctga gtccccttcg tcgccttcac gaattaacgt ctcacctgcc tgcacaacga
     2041 cgtttatgga taacgtcgcc agccaatcaa gatcctctgg agaaagacta gccagcaagg
     2101 gcgctttttg caggagctca gcgttcatca cacgacctaa atggttttca ggtattgccg
     2161 gatcaaggcc accgccaggc cgccttcgct ggtggcagct gaaacgcggt gggttgtgcc
     2221 gtaacgaaca tctcccgccg caaaaatacc cgggacgctg gtttccagca ggtatggatc
     2281 gcggtccaga ggccaatttg ccggccgctt gccctcgaca aggagatccg gcccggtgag
     2341 cacgtagccc ttctcagccc gcgccaccag atcggcaacg aactggctct ggggtctcac
     2401 cccaataaac acaaacatag ctgccccttt gagggtctga agctcgtcag tacggttatt
     2461 cttcaccact atctcttcta gttttccctc tccgcgcaca tcgatgaggt ctgtgttgag
     2521 ataggtctcg atcttttcat tctggcggag ctcatccgcc aggtattggg cagcgctgag
     2581 ctcaccccgc acaattaagt tcaccttacg cgcaaaacgg ctcaggtaca atgc
//
LOCUS       DBNGSX010000165         4758 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1467064, whole genome shotgun sequence.
ACCESSION   DBNGSX010000165 DBNGSX010000000
VERSION     DBNGSX010000165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1467064"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1973
                     /locus_tag="AC16CB_05280"
     CDS             117..1973
                     /locus_tag="AC16CB_05280"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3341913.1"
                     /translation="MLPYLFLLIAALAQLAVPRLVRNIIDAVAQGVMAKSMLAGLNQI
                     PAALLLEALPTILQTFGLPNDWTAEQLLSKLATDIANAPRLMVSAAVAIVIFAALRGL
                     FAFLQVYLAERNSQSVAFDLRNDLFAKIQRLSFSYHDRNQTGQLMIRATDDVEKVRIF
                     IGQGLLQLAGALVLLAGTVILLLVTNLRLALVILPILPVALVLFILFGSLSQPLFAKV
                     QIKLSTLNTVLQENLAGIKVVKAFTRERSEQVKFQTAADALMHQQIGISRLFTFLFPL
                     VFLTANLGQASILYFGGRQIIAGTLSLGEWQEFSLYLIYLFLPIAQLGIIITQFGQAS
                     ASAARIFEILDAHSDVSDLPGAIQLPPVQGSIRFENVTFRYFSSGEPVLKNVSFEALP
                     GQTVALLGATGSGKTTIINLLPRFYDPSEGRISIDDHDLRQVTLDSLRSQIGIVLQET
                     TLFAGTIRDNIAFGKPEASQEEIIAAAKAAAAHDFIMSFPQGYDTPVGERGTTLSGGQ
                     KQRIAIARALLLDPHILILDDSTSSVDLATEAQIQAALDDLMKGRTSFVIAQRISTVM
                     NADLILVLDKGAIVARGQHAELMEESPIYAEIYNSQLIGDVEVSVSPLASEA"
     gene            2069..4144
                     /locus_tag="AC16CB_05285"
     CDS             2069..4144
                     /locus_tag="AC16CB_05285"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3341914.1"
                     /translation="MFGGQDGLRRVMSRETIKPRKVGETLARLGGYFKPYWYALVLTA
                     ILAVASTWAQVVTPELTGQVVDCFLTPAAGSAFTSFSGLPGTPVSAGQNGAAAANCWL
                     AAGKPSAGLSQTIVKSLLSVVSFQAPNLEPGQMNTAERLIGMGQLILLITIIFIFGSI
                     LVGLTFFTMSWAGQHVLRSLRVDVFRHLHRLPLSYYAEQEAGDLMSRITNDSEAIQQA
                     LSFALVNVATGALLLGWIAYNMLARSLVFGLLSLIVVPIMSIATFWFSDQARKAFRRT
                     RQEMGSVNAELQESIAAVREVQAFNRAEENIENFRITNAANRDANIRAVAFTSALAPT
                     LEALGYLALAIVTGVGGLALLRDEPLFGTAVSLGLVITFLGYVQRFNQPIQQIAVLWT
                     NLQNAIAGGERIFNLLDVEPAIQDSPNAQPMPTIQGRVEFNNVYAAYTKGQPVLEGVN
                     FIAEPGQTIAIVGPTGAGKTTIINLIPRFYDVTGGAVLIDGMDVRYATTESLRQQIGI
                     VLQDTFLFSTTVMDNIRFGHPEASDEEVIAAARLAHADSFVERLPEGYQTVLGERGSG
                     LSQGQRQLIAIARAALADPRILILDEATSSVDTRTERLIQKALEKLLEGRTSFVIAHR
                     LSTIRNADQLLVLDMGQIVERGVHSELLERKGFYYDLYMSQFKRQDETVAAGGNGRHA
                     LKTKSQTPG"
     gene            complement(4248..4323)
                     /locus_tag="AC16CB_05290"
     tRNA            complement(4248..4323)
                     /locus_tag="AC16CB_05290"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4288..4290),aa:Arg,seq:cct)
     gene            complement(4396..>4758)
                     /locus_tag="AC16CB_05295"
     CDS             complement(4396..>4758)
                     /locus_tag="AC16CB_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3341915.1"
                     /translation="MPYLVNDDPVPNQERLQSPEQRERFDDTTKCILCAACTTSCPSF
                     WASDKYIGPASIVNAHRFIFDSRDQAGAERLSLLTDQWGVYRCHTIFNCTEACPREIQ
                     VTQAIGEVKQAIATGKID"
BASE COUNT         1018 a         1387 c         1338 g         1015 t
ORIGIN      
        1 gacgggaaaa ggagaacttt ttgcgaaaca gacgcattag caactcgcaa cgcactatcc
       61 agcgccaagc gttgggtcgg gcggtgcgtt acttggcacg ctaccgaagg caggcagtgt
      121 tgccctacct cttcctgctc atcgccgcgc tggcacaact ggcggtgccg cgcctggtgc
      181 gcaacatcat cgatgcggtg gcgcagggcg tgatggcaaa atccatgctc gccgggctaa
      241 atcagatccc cgccgccctt ttacttgaag ctttgcccac catcttgcaa accttcggcc
      301 tgccaaacga ttggaccgca gagcaattgc tgtccaaatt ggcaaccgac attgccaatg
      361 caccccgctt gatggtcagc gcggcagtcg cgatagtgat ctttgccgcc ctgcgaggcc
      421 tgttcgcttt tctgcaggtc tacctggcag aacggaactc gcagagcgtg gccttcgacc
      481 tgcgcaacga tctgtttgcc aagatccagc gcctctcatt ctcctaccat gaccgcaacc
      541 agaccgggca gttgatgatc cgcgccacag acgatgtgga aaaggttcgt attttcatcg
      601 gccagggctt gctgcaactg gcgggtgcgc tggtgttgct ggcgggcact gtgatactgc
      661 ttttagtgac caacctgcgc ctggcgctgg tcatcctgcc tatcctgccg gtcgccctgg
      721 tcttatttat actcttcggc tccctcagcc agcctttatt cgccaaggtg cagataaagc
      781 tctcgacgct gaacaccgtg ctgcaagaga acctggccgg catcaaggtg gtaaaggctt
      841 tcactcgaga gcgaagcgag caggtgaaat tccagacggc tgccgatgcc ttgatgcacc
      901 aacagatcgg tatctccagg ttatttactt ttcttttccc gctggtgttc ttgaccgcca
      961 acctgggtca ggcctccatc ctgtattttg gtggcaggca gattattgcc ggcacgctat
     1021 cgctgggcga gtggcaggag ttcagcctgt acttaattta cctgttttta cctattgccc
     1081 agctcggcat catcatcacg caatttggtc aagcttccgc ctccgccgca cgcatcttcg
     1141 aaatcctgga tgcacacagc gacgtgagcg atctgcctgg tgccatccaa ctgccgcctg
     1201 tacaagggag catccgcttc gagaatgtca ctttccgata tttcagtagc ggagaaccag
     1261 tgctgaaaaa cgtgagcttc gaagcgttgc cagggcagac cgtcgccctg ctgggcgcaa
     1321 ctggctctgg taaaaccacc atcatcaacc tgctgccgcg cttctacgat cccagcgagg
     1381 ggcgcatttc cattgacgat catgacctgc gccaggtgac cttggattcg ctgcgctcgc
     1441 agatcggaat tgtcctgcaa gagaccaccc ttttcgccgg caccatccgg gataacatcg
     1501 cattcggcaa gccggaagcc agccaagaag agataatcgc cgccgccaaa gccgcagcag
     1561 ctcacgactt tatcatgtcc ttcccgcaag gctatgacac cccggtggga gagcgcggca
     1621 ccacgctaag cggcgggcag aaacagcgca ttgccatcgc cagagccctg ctgttggatc
     1681 cacatattct catcctggac gactcgacca gcagcgtcga cctggcaacc gaggcgcaaa
     1741 tccaggctgc cctggacgac ctgatgaaag ggcgcaccag cttcgtcatt gcccagcgca
     1801 tcagcaccgt gatgaatgct gacctgatcc tggttctgga taagggcgct atcgttgccc
     1861 gaggtcagca tgccgagttg atggaagaaa gccccattta tgccgagata tacaactctc
     1921 aactcattgg ggatgtggaa gtttcagtgt caccgctggc gagtgaagcg tgaccgcact
     1981 tcgaaagaca agcagcatcg tgcgcgaaca ttacacgccg cacaatgtag gacaaggaat
     2041 tctataagaa ggtatttggg agcaaaacat gttcggtgga caagatggat tgcgccgcgt
     2101 gatgagccgg gagacgatca agcctcgcaa agtgggcgag accctggctc gcctgggcgg
     2161 ctattttaag ccttattggt acgcattggt gcttacggcg atactggcag tggcttccac
     2221 ctgggcacag gtggtcacac cagagttaac tgggcaggtg gtagattgct ttctgacacc
     2281 cgcagcggga agcgccttta ccagcttctc ggggctgcca ggaacgcccg tctcggctgg
     2341 gcagaacggc gcggcggctg ccaactgctg gctggcagca ggtaaaccat cggcaggcct
     2401 cagccagacc atcgttaaaa gcttattgtc tgtagtcagc tttcaggctc ccaatttgga
     2461 acctgggcag atgaacaccg ctgagcgcct gattgggatg gggcaattga tcttgctcat
     2521 tacgattatc ttcatctttg gatcaatcct ggtcggcctg acctttttca ccatgagttg
     2581 ggctggccaa cacgtcttgc gctccctgcg cgtggatgtc ttccgtcacc tgcaccgcct
     2641 cccactgagc tattacgccg agcaggaagc tggcgatctg atgagccgca taaccaatga
     2701 ctccgaggcc atccaacagg cactcagctt cgccctcgtc aacgtggcaa ccggggcgct
     2761 gctgctgggc tggatcgcat acaatatgct ggcgcgcagc ctcgttttcg gcctgctcag
     2821 cctgatcgtc gtgccaatca tgtccatcgc cacgttctgg ttctcagacc aagcgcgcaa
     2881 agccttccgc cgcacccgcc aggagatggg cagcgtaaac gccgagctgc aggagagcat
     2941 cgcagcagtg cgagaggtgc aggctttcaa ccgtgctgag gaaaatattg agaacttcag
     3001 gattaccaat gccgccaacc gggatgctaa tatccgcgcg gtggccttca ccagcgcttt
     3061 ggcgcccacc ctagaggcac tgggctacct ggcgctggct atcgtcaccg gcgttggcgg
     3121 gctggctttg ttacgcgacg agccgctctt cggcacggca gtctcgttgg gtttggtgat
     3181 cacctttctg gggtacgtgc aacgcttcaa ccagcctatc cagcagattg ccgtgctgtg
     3241 gacaaaccta cagaatgcta ttgctggtgg ggagcgcatt ttcaacctcc tggacgtgga
     3301 gcccgctatc caggactcac ccaatgccca acccatgccc accatccagg gcagggtgga
     3361 attcaataat gtctatgccg cttatacgaa ggggcaacct gtattggaag gcgtgaactt
     3421 catcgccgag ccgggtcaga ccatcgccat cgtggggccc accggagcag gcaagaccac
     3481 gatcatcaac ctgatcccgc gcttttacga tgtgacaggt ggagcggtat tgatcgacgg
     3541 gatggatgtg cgctatgcca ctaccgaatc gctgcgccag cagatcggca tcgtgttaca
     3601 ggatacgttc cttttcagca cgacagtgat ggataacatc cgattcgggc acccggaagc
     3661 cagcgacgaa gaggtgatcg ctgccgcccg cctggcacat gcagacagct tcgtcgagcg
     3721 cttgcccgag ggctaccaga ccgtactagg tgagcggggc agcggcctga gccagggaca
     3781 gcgccagttg attgccatcg cccgcgccgc cctggctgat ccgcgcattc tcatcctgga
     3841 cgaggccacc tcaagcgtgg acacgcgcac cgagcgcctg atccaaaaag cccttgagaa
     3901 gctgctggaa gggcgcacta gctttgtcat cgcccatcgc ctgagcacca tccgcaacgc
     3961 tgatcaattg ctggtactgg acatgggtca aattgtggaa cggggggtgc acagcgaatt
     4021 gctggaacgc aaaggctttt actacgacct gtatatgagc caatttaagc gccaggatga
     4081 gacggtcgcg gctggtggga acgggcgcca cgccctgaaa acaaaaagtc agacgccagg
     4141 gtgagggggg caccctggcg tctgacaagc aatatattac tgcaactcct cagaagaatc
     4201 aagacccatt tcattgcaat ttcgttgcaa tttgcaattc ttaatcttgg tgccccaggc
     4261 gggagtcgaa cccacaacct cggccttagg agagcctcgc tctatccatt gagctactgg
     4321 ggcgtgcgag gggattatag cataaccaac aaaaaaactt cccgcaggct ctgaacctgc
     4381 gggagaaatt tctcactaat cgatcttccc tgtggcgatg gcttgtttta cctcgccgat
     4441 ggcttgcgtc acctgtatct cgcgcggaca ggcttctgta cagttgaaaa tagtatggca
     4501 gcggtacaca ccccattggt cagtcagcaa actgaggcgc tcggcacctg cctggtcgcg
     4561 gctgtcgaag atgaagcggt gggcgttgac gatcgacgca ggtcctatat atttatcact
     4621 tgcccagaat gaagggcagg aggtagtgca ggctgcgcac agaatgcact tggttgtatc
     4681 gtcgaagcgc tcgcgctgct cagggctctg caagcgttcc tgattgggta ctgggtcatc
     4741 gttgaccaga tagggcat
//
LOCUS       DBNGSX010000166         2403 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_843843, whole genome shotgun sequence.
ACCESSION   DBNGSX010000166 DBNGSX010000000
VERSION     DBNGSX010000166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_843843"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="AC16CB_05300"
     CDS             complement(<1..390)
                     /locus_tag="AC16CB_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR3341916.1"
                     /translation="MQTEIVIAGFGGQGVLFAGQVLAYAAMDNGKEVTWIPSYGPEMR
                     GGTANCTVIIADEEIGSPLVLNPKAAIVLNLPSLDKYESLIQPGGVLVANASLINRGP
                     SRSDIKSVFIPANEIAESIGNKRLTNMI"
     gene            complement(404..1180)
                     /locus_tag="AC16CB_05305"
     CDS             complement(404..1180)
                     /locus_tag="AC16CB_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3341917.1"
                     /translation="MELSAQAQIVYQRPESLADVPSHYCPGCTHGIAHRLLAEVLDEM
                     GVRENTIGVAPVGCSVFAYNYFDFDFVEAAHGRAPAMATGIKRLLPERVVFTYQGDGD
                     LASIGMAEIMHAAGRGENITVVFINNAIYGMTGGQLAPTTLPGQKTTSSPSGRDVEVM
                     GFPLRMSEVLSTLDGAGYIVRRSLHDPKNIRMAKKAIRLAFETQKRGLGFSMVELLST
                     CPTNWGITPLEALRWLEEHMIPVYPIQDFKVSSTIARLRI"
     gene            complement(1193..2326)
                     /gene="vorB"
                     /locus_tag="AC16CB_05310"
     CDS             complement(1193..2326)
                     /gene="vorB"
                     /locus_tag="AC16CB_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HIR3341918.1"
                     /translation="MPRELLKGNNAMAEAAIRAGVEAYFGYPITPQTELLEHFSQRMP
                     ELGRVFLQAESEVAAINMVYGAACTGARVMSSSSSPGVSLMLEGISYIAGTEIPVVLI
                     DVVRGGPGLGNIAPSQGDYNQIVHGGGHGDYHPIVLAPASVQEAIDLTVLAFDLADQY
                     RTIVIVLADGNIGQMMEPAELPPMRPLRKEYPDWAVRGASGRERRILTSIYIDPKDEE
                     VVNYRLMKVWNQIQANEVRYKEYYLDDAEFVVVGYGTAGRVALSAVREARSQGIKAGL
                     FRPVSLSPYPYAQLEALTDRTQAFLVTEMNGGQMLDDVLKAVKGRVPVEFYGRMGGMV
                     PFPDEILAEIQRIAQEPLSIKENPRLDWMKRIAGIIDAARSRN"
BASE COUNT          560 a          706 c          592 g          545 t
ORIGIN      
        1 aatcatattg gtcaagcgtt tgttgccaat cgattcggca atctcgttgg ctgggatgaa
       61 aaccgatttg atatcactcc gagatggacc acgattgatc aatgaggcgt tggctaccaa
      121 gacacccccc ggttggatga ggctttcata tttatccagc gaaggtaggt tgagcacaat
      181 ggctgccttg ggattgagca ccagaggcga ccctatctct tcatcagcaa taatgaccgt
      241 gcagtttgcg gtgcccccac gcatttctgg cccataagaa ggaatccagg tcacctcttt
      301 tccgttatcc atagcggcat aggctaaaac ctgccctgcg aacagtactc cctgcccacc
      361 aaatcccgcg atgacaattt ccgtttgcat cgtgatcatc tccctaaata cgcagccttg
      421 ctatagttga ggacactttg aagtcctgga tgggatagac gggtatcatg tgctcttcca
      481 gccagcgcaa tgcttctaag ggagtaatac cccagttcgt cggacaggta gagagcaatt
      541 caaccattga gaaccccagc ccacgcttct gcgtttcaaa ggctaaacgg atggcttttt
      601 tagccatgcg gatattcttc ggatcatgca gacttcgccg cacaatgtaa ccggctccgt
      661 ccagtgtcga taatacctcc gacatgcgta gagggaagcc catcacctcc acatcgcgcc
      721 cgctagggga ggaggtcgtc ttctggcctg gaagggtggt gggagctaac tgcccccctg
      781 tcatcccgta aatggcgttg ttgataaaaa caacggtgat attctccccg cgacctgcag
      841 cgtgcataat ctctgccatg ccaatagacg ccaggtcgcc atccccctga tatgtaaaaa
      901 ccactcgttc ggggagcagg cgcttgatgc ccgttgccat ggccggagcg cgcccgtgcg
      961 ctgcctccac aaagtcaaaa tcaaaataat tgtaggcaaa aacagagcag cctaccggcg
     1021 ctacaccaat cgtgttttca cgcaccccca tctcatccag cacttctgca agtaggcggt
     1081 gtgcaatccc atgagtacac ccaggacaat aatgggatgg cacatcagct aaagattcgg
     1141 ggcgttgata gacaatttga gcctgagctg acaattccat tagcagagct cctcagttcc
     1201 ttgacctagc cgcgtcgatg attccagcaa tacgcttcat ccaatctagg cgcggatttt
     1261 ctttgataga tagcggctcc tgtgcgatgc gttgaatttc cgccagaatc tcatccggga
     1321 acgggaccat accgcccatg cgcccgtaaa actctactgg cacgcgaccc ttgaccgcct
     1381 tgaggacatc atccagcatc tgaccgccgt tcatttcggt caccaagaaa gcttgggtgc
     1441 ggtctgtcaa ggcctccagt tgggcatatg gataggggct gagtgagact ggccggaata
     1501 agcctgcctt gatcccttga ctgcgcgcct ctctaactgc agacagggca acgcgccctg
     1561 cggtgccgta acccacaacg acaaactccg cgtcatccag ataatactcc ttgtagcgca
     1621 cctcattggc ctggatttga ttccagactt tcatcaggcg atagttcacc acctcttcat
     1681 ctttcggatc gatgtaaatc gaggtgagaa tgcgccgctc gcggcctgag gcaccgcgta
     1741 ccgcccaatc gggatattcc ttgcgcaatg gacgcatggg aggtagctct gccggctcca
     1801 tcatctggcc gatatttcca tccgctaaga cgatcacaat cgtgcgatat tgatctgcca
     1861 agtcgaaggc aagcaccgtc agatcaattg cctcttgcac actggcaggc gctaacacga
     1921 tggggtgata atcgccatgc ccgcctccat gcactatttg gttgtaatcc ccctgagagg
     1981 gggcaatgtt tcccagacca ggcccgccac gcaccacatc aattaacacc actggtatct
     2041 ctgtccctgc aatgtaggaa ataccttcca acattaagct tacaccgggg ctggaggatg
     2101 aactcatcac tctcgccccg gtgcaggctg ctccgtacac catgttgatg gcagcaactt
     2161 cactctctgc ctgcaggaag acacgcccaa gctcgggcat acgctgggag aaatgttcca
     2221 gcaactcggt ttgaggtgtg attgggtagc cgaaatacgc ctcaacgcca gcgcggattg
     2281 ccgcttcggc catagcattg tttcccttaa gcaattctcg aggcatacgt cctcctaagc
     2341 tgccgccgct ctcgcagcac gcacaggggt ctcgcgataa accgtcagcg ctgcctcggg
     2401 aca
//
LOCUS       DBNGSX010000167         2901 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_636240, whole genome shotgun sequence.
ACCESSION   DBNGSX010000167 DBNGSX010000000
VERSION     DBNGSX010000167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_636240"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1618
                     /locus_tag="AC16CB_05315"
     CDS             <1..1618
                     /locus_tag="AC16CB_05315"
                     /inference="COORDINATES: protein motif:HMM:NF037660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR3341919.1"
                     /translation="LINEAIDHSLDGEDFQHAAQLIESISPDMMMQNEFDQLEKWLDA
                     IPQELVETWPWLCIIRAWMNQRWARLDEAEQYIIYAEQALEAETIQKPVEGANVIRGQ
                     AAAIRALVALNKTRIPQAIEFANHALNLLPVGHFNRAISMLVLGMAKGANGEYVEANQ
                     ILVEAYRDSLNVGNLTQAQNILLENGILLEKQGRLHQALETYEEAVQLTYSKTEIKLP
                     HASFASVFMGNIYREWNELDAALRHVKEGIEIGLPFKMVDAVATGYAVITRIHLALDD
                     LESAIEAGLIAGRYVRELPGLEPITITQIWDSEARVKFAQKKIDEAASLFLENNPCDV
                     YDINCFGEFEQIIWARILLYRGRECKNVSDLLKAQDLINQMLELTKDLGLTSKTIELL
                     VLNSLVLIEIETMDGALDALAEALWLAETGSYMRVFIDEGDSMKALLREAETRGIAPD
                     YVSKLLAGFETDRVPVEITSVHSLIEPLSEREIEVLRLLKTELSGPDIADVLMIALST
                     LRTHTQNIYGKLGVSNRRAAVRKAEELNLR"
     gene            1817..2536
                     /locus_tag="AC16CB_05320"
     CDS             1817..2536
                     /locus_tag="AC16CB_05320"
                     /inference="COORDINATES: protein motif:HMM:NF025687.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HIR3341920.1"
                     /translation="MNSETDTSKFADTSQRTAAIAVGIGLLASILGAVFVELTTNSLI
                     VADDAATTARNIMASEALYRAGIFGWVVAILGDMLRAWGLYVFFKRVNQSLALLSVWF
                     MLVHDAIFAASLVNLYFGSVFPSSADLLTVFEAEQLQALGMLVLNGFEYGFHMGLFFF
                     SFHLGVVGYLAYKSGFVPKILGVLMMAAFLSYLIDSFAFLFLPIYPELLNQFLMLPEI
                     IGEVAIAVWLAFKGGKGATDK"
     gene            2609..>2901
                     /locus_tag="AC16CB_05325"
     CDS             2609..>2901
                     /locus_tag="AC16CB_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007694271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HIR3341921.1"
                     /translation="MKNLQKMGGVAALYEAAAYLIALVGYLLVVGTMDVVDPVEKVSS
                     LVNNQAFLYILNLIAYIFWGFFQIALALALYERLKAGSPALVQTATGIGLVWA"
BASE COUNT          788 a          602 c          727 g          784 t
ORIGIN      
        1 tcttatcaac gaagccatcg accattcttt ggatggtgag gattttcagc acgctgccca
       61 gttaattgaa agcatcagcc cagatatgat gatgcaaaat gaattcgacc aattggaaaa
      121 atggttggat gcaatacccc aagaactcgt ggaaacttgg ccatggcttt gtattattcg
      181 agcctggatg aatcaaagat gggcacgtct agatgaggca gagcagtata ttatatatgc
      241 tgaacaagct ctggaggctg aaacaatcca aaaacctgtg gaaggagcca acgtaatacg
      301 gggccaagcg gctgcaatcc gtgcattagt tgccctcaac aaaacacgaa tacctcaagc
      361 aattgaattt gcgaatcatg cactaaattt attacctgtg gggcatttta acagagccat
      421 ttcaatgttg gtgctgggta tggcgaaagg ggcaaacggc gaatacgttg aagctaacca
      481 gattttggtt gaggcataca gggatagctt gaatgtaggt aacctaaccc aggcacagaa
      541 tatattgctc gaaaacggga ttttattgga aaaacaagga cggcttcatc aagcattaga
      601 aacttatgaa gaagctgtac agcttacata ttcaaaaacg gagattaagt taccccatgc
      661 aagttttgcc agtgtattca tggggaatat ttatcgagag tggaatgaac tggatgctgc
      721 cttgagacat gtaaaggaag gtatcgagat tggtttaccg ttcaaaatgg ttgatgccgt
      781 tgcgacaggt tatgcggtta taacccgaat acatcttgcc ttggatgacc ttgaatcagc
      841 cattgaggcg ggtcttattg ctggaagata tgttcgcgaa ttaccaggtt tagagccgat
      901 taccatcacg caaatttggg atagtgaggc gcgggtgaaa tttgctcaaa agaaaataga
      961 tgaggctgcg agcttatttc ttgagaataa cccgtgtgat gtatatgata tcaattgttt
     1021 tggtgagttt gagcaaataa tttgggccag gattctcctt tacaggggga gagagtgtaa
     1081 aaatgtgtcc gatttattga aagctcaaga cttgattaat caaatgttgg agctgaccaa
     1141 agatttggga ctgacaagta aaacgataga gctcctggtg ctgaattccc tcgtcctaat
     1201 tgagatcgaa acaatggatg gcgcgttaga tgctttagca gaagctttat ggcttgcaga
     1261 aaccggaagt tacatgcgcg ttttcatcga tgagggtgat tcgatgaaag ctttgctgcg
     1321 tgaagctgaa accaggggta tcgcacccga ctatgtctct aaattgctgg cgggatttga
     1381 aactgatcgt gtcccggtgg aaataacctc tgtccattca ctcatcgaac ccctgagcga
     1441 gcgagaaatt gaagttctgc gcttgctcaa aacagaatta tccgggccag atatagctga
     1501 tgtgctgatg atcgccttga gcacattgcg cacccacacg caaaacatct atggcaaatt
     1561 gggcgtgagt aaccgccggg cggctgtccg taaagcggaa gaacttaact tgcgataatc
     1621 aatacatatc acaactgcca ttctgccctg cctggatatg ggtcaacata gctgcttcaa
     1681 ggcagggctt tttttgtccc tcttggctta actgatacag taacaaaaat catcacatgt
     1741 gatgatgaca tctccacaca ttaatttgta tattccaatt gataggatcg attggaaaag
     1801 ctagagagga gaaaaaatga attcagaaac agatacctca aaatttgccg acacatcaca
     1861 acgcacagct gcaatagccg taggcatcgg attactagcc tcaattctag gcgctgtctt
     1921 cgtcgaactc actacgaaca gtttgattgt ggcggacgat gcggcgacaa cagccagaaa
     1981 tatcatggca tcagaggcgc tatatcgcgc cggtattttt ggctgggtgg tcgcaattct
     2041 gggtgacatg ctgagggctt ggggacttta tgtctttttc aaacgcgtta accagagtct
     2101 ggcgttgctc tcagtatggt tcatgttggt tcacgatgcg attttcgcag cctcgttggt
     2161 caatttgtat ttcggttcgg tctttcccag cagtgctgat ctcttgaccg tatttgaagc
     2221 tgaacagcta caggcgctgg gcatgctagt cctgaatggg tttgaatacg gattccatat
     2281 gggactattc tttttcagct ttcacctcgg tgtcgtgggt tatttagcat acaagtcagg
     2341 cttcgttccc aagattctgg gcgtcttgat gatggctgca ttcctcagct atctaattga
     2401 cagctttgca ttccttttct tgcccatcta cccagagcta ttgaatcagt ttctaatgct
     2461 gcctgagatc attggggaag tggcgattgc tgtgtggctt gccttcaaag gtggtaaagg
     2521 cgcaacagac aaataactgt gctcttgaat caacccgaat aagactcatg ccgaaggaat
     2581 caatcaataa caataaagga gaaatgaaat gaagaattta cagaagatgg gcggcgttgc
     2641 cgcactgtac gaggcagcag cctacttgat cgctctggtg ggttacctct tggtagtcgg
     2701 cactatggac gttgtcgacc ctgtcgagaa agtgtcttcg ctcgtgaaca accaggcctt
     2761 cctgtacatt ttgaacctga tcgcctatat attttggggc ttctttcaga ttgccctggc
     2821 gctagcgctc tacgagcgct tgaaggccgg ctcacccgca ttggtgcaga cagcgactgg
     2881 cattgggctt gtctgggcct g
//
LOCUS       DBNGSX010000168         8379 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1882550, whole genome shotgun sequence.
ACCESSION   DBNGSX010000168 DBNGSX010000000
VERSION     DBNGSX010000168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8379)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8379)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8379
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1882550"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="AC16CB_05330"
     CDS             complement(<1..431)
                     /locus_tag="AC16CB_05330"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004939904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR3341922.1"
                     /translation="MNLQDANTPAPGPDNQPGQETAPATAGGQRYVVVKMPPTKPYVT
                     YAFMGFTILVYLLQLGSKFFLGGLDYPAALGLKANDAILQGELWRLFTPMFLHSTSSL
                     LHIGFNMYALYALGPELERHFGHLRYLALYLLSGFAGNVAS"
     gene            complement(428..1051)
                     /locus_tag="AC16CB_05335"
     CDS             complement(428..1051)
                     /locus_tag="AC16CB_05335"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR3341923.1"
                     /translation="MTERNIFNSHQDQPLLIVISGPSGAGKDTVIEHMKQRELPFYFV
                     VTATTRPPRPNEVHGVDYFFFSKDEFAGMIERGELMEYALVYKDYKGVPKEQVRRALA
                     SNQDVVMRLDVQGAATVRKLAPEAVLIFLTTSSEEELITRLEARMTESPEGLKLRIAT
                     ARSEMGRLAEFDYVVVNAQNQLDETVDTILAIIKAEHHRVLPRMVNL"
     gene            1239..1634
                     /locus_tag="AC16CB_05340"
     CDS             1239..1634
                     /locus_tag="AC16CB_05340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR3341924.1"
                     /translation="MPFYQYHCLNCRRRFDTFMSYSEYGTKSVHCPNCNSDQVRRRIG
                     RVRVARSEESRMEDLADPGNLAGLEDDPKALGRMMRKMSREMGEDMGPEFDDVIDRLE
                     SGQSPEEIEKAIPDLGGEGGGAGGLDEDF"
     gene            complement(1772..2680)
                     /locus_tag="AC16CB_05345"
     CDS             complement(1772..2680)
                     /locus_tag="AC16CB_05345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3341925.1"
                     /translation="MKPLTNKVAVVAGATRGAGRGIACMLGEAGATVYCTGRSTRARL
                     KSRRSGNMAPFALEGRPETVEETAELVSRYGGVGIPVQVDHTDEEQVEHLFVQVQGEQ
                     GKLDILVNDIWGGDELSEWGQPFWELSIQKGLLMQQRGVHTHIITSRYAVPLMVARRR
                     GLIIEITDGDSAAYRGNLFYDLAKSSVIRLAYAMAEELRPHNITALALTPGFLRSEAM
                     LEHFGVTEANWQEGATKDPHFIASETPYYIGRAVAALAADPNVFQMSGKALSSWNLAL
                     EYGFKDIDGRQPNWGKYFEEHIAGKM"
     gene            complement(2689..3051)
                     /locus_tag="AC16CB_05350"
     CDS             complement(2689..3051)
                     /locus_tag="AC16CB_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012694571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR3341926.1"
                     /translation="MAKILVHVTYGPEHPTRAALAFLVAKSAIDEGHTVTLFLAGDAI
                     QLLRDAVLDTLAGLGTGKLRDSYDAIVAGGGRFYLSGMSSKARGLNEADLAGKPAEFA
                     MPNVLVRLSLENDRMFTY"
     gene            complement(3171..3596)
                     /locus_tag="AC16CB_05355"
     CDS             complement(3171..3596)
                     /locus_tag="AC16CB_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3341927.1"
                     /translation="MFLYYFSGGLLVFSTVLYHVTQKMTPQDANPALALTVTYMSSLI
                     LCILLFFLFPVSSNLRTAFSQLNWASFGLAFALLGLEVGFLLAYRAGWNISIAAIFVN
                     VAGILLLVPIGLFFFKERLSLINLLGILVCIAGLIMVNW"
     gene            complement(3626..3757)
                     /locus_tag="AC16CB_05360"
     CDS             complement(3626..3757)
                     /locus_tag="AC16CB_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341928.1"
                     /translation="MGYKGPSAPRSVAEGADTIVWLATLPDGGPSGQFFRDQAQIRW"
     gene            complement(3747..4538)
                     /locus_tag="AC16CB_05365"
     CDS             complement(3747..4538)
                     /locus_tag="AC16CB_05365"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3341929.1"
                     /translation="MKHVVITGSTRGLGFGLADAFLELGCSVTVSGRMQSSVAAAVGK
                     LNEKHDSSRISGCACDVRHLEQIRALWDASIERFKQVDVWINNAGFSGPQLSAWKIPP
                     DQAQDIIQTNLLGVIYGSMVSIKGMLGQGYGSLYNMEGMGSDGRKHNGLALYGTTKYG
                     VHYFTECLVNEIQGTPLVVGSIRPGMLITDMILKQYEDGSPDWERAKRVFNLLADRVE
                     TVAPWLAAQILANQRSGACIRWLTKGKLMRRMLFSPFQKRDLFGV"
     gene            complement(4606..5964)
                     /locus_tag="AC16CB_05370"
     CDS             complement(4606..5964)
                     /locus_tag="AC16CB_05370"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR3341930.1"
                     /translation="MKTIKFVLINPTSPLWRVRGGERPRGSRYFRFSMLPSLYVAASM
                     PANVETQIIDEDVESIDFDVDADLIGVSFMTYNAPRAYEIADIFRTQKGKPVIFGGYH
                     PTFMPEEAIQHADAVCIGEAESNVPRMIDDFLSGDLKPFYHSEPVRLEALPKLNRNLI
                     RKKDYAPVDVLQATRGCFYRCSFCSVAAFHQYCFRTRPVADVIADLKTLGPHVLFMDD
                     NLIGDREYARELFSAMIPLGKRWFSQCGVGIASDDELLRLAARSGCGGLFIGFESLSQ
                     EGLRSWKKRTNLGKDYMEVVRRLHQAGMAVFAGFVFGGDEDTPDVFEQTLEFLLEANI
                     ETLQATRLTPFPGTTLFEELDGQGRIFDKDWSHYDFNHVVFEPRQMDRETLDNGVAWV
                     LREFHTRSRVARRLWKSLGYLHPQVVLRGVLPLNVGFRYKLSMDGNFQRGVDFAPPSM
                     MP"
     gene            complement(6058..7671)
                     /locus_tag="AC16CB_05375"
     CDS             complement(6058..7671)
                     /locus_tag="AC16CB_05375"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR3341931.1"
                     /translation="MKDARPKTLSGGRHRLGLPSLVATLVLVFSMLACSTGSYWVPPK
                     GTDTPPPDISFLLEPTPTEITILDPLPGDIATPVTDPPLPQDTPVPPAINTTPVLYYT
                     QAADTLPVVAVRFGVRPEEITSPDPVPEEALLPPNQLLIIPRRLVNAASSQRIFPDSE
                     VVYSPSAIDFDIQAYVQEAGGYLSTYKEWLQTTKTTTGADIVARVAVENSINPRLLLA
                     LLEYQSGWVYGQPSDLKMADYPMGLVNLNKKGLYQQLVWAINHLSVGYYSWREGRLLE
                     LRFADGVTARLAPDVNAGSAALQYYFSQIYTGEQWVRAIDLQDGLPALYERMFGSPWM
                     RAQAVEPLYPPGLAQPELILPFEVDRLWSYTGGPHGAWEHDGSYAALDFAPASSEPGC
                     QQSNAWVTASASGLVVRSGNGVVVLDLDGDGYEQTGWVVLYLHVSTQSRAPVGTWVGA
                     GDHLGHPSCEGGMATGTHVHIARKYNGEWIAADGPMSFQLSGWMAHAGDQAYKGSLTR
                     DGETIPACTCASFQTNITRSENDPYEAGS"
     gene            complement(7634..>8379)
                     /locus_tag="AC16CB_05380"
     CDS             complement(7634..>8379)
                     /locus_tag="AC16CB_05380"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR3341932.1"
                     /translation="LLGDGSVVPVASTINAGTAGVQHMFAALYGRADWEQAVGENGVF
                     SVYNTFFGYPFDYAIEPLLSPGLRQPPMQLPFEPEVAWAFTGGPHGGWGDGSAWAALD
                     FAPPSDLLGCIVSNDWVTAVADGLILRAENGAVIQDLDGDGYEQTGWVVLYMHVESRE
                     RVSPGTFLKAGERIGHPSCEGGISSGTHVHLARRYNGEWIPADQNLPFVLDGWVSRGA
                     GLEYDGYLQKDGYEVEAYEGRSAENAIRR"
BASE COUNT         2009 a         2399 c         2238 g         1733 t
ORIGIN      
        1 aaggaggcca cattcccggc aaagccactt agcaggtaca aagccagata gcgaagatgc
       61 ccaaaatggc gctccagctc tggccccaga gcgtacaaag catacatatt aaaaccaatg
      121 tgcagcaggg agctggtgga atgcaggaac attggggtga acaagcgcca aagctcgccc
      181 tgcaaaatgg cgtcgttagc tttcaagccc aacgctgccg gataatctag cccacccaag
      241 aagaacttgc tccccaactg caacagatag accaggatgg taaaacccat aaaagcatac
      301 gtgacatagg gcttggtggg aggcatctta accactacat atcgctgccc gcctgcagtg
      361 gctggcgcag tttcctgacc tggctgattg tcgggaccag gtgcaggtgt gtttgcgtct
      421 tgcaggttca caggttcacc atccggggga ggacgcggtg gtgctcagcc ttgatgatcg
      481 ccaagatagt atccaccgtt tcgtccagtt ggttctgggc gttaaccacc acatagtcaa
      541 actcagccag acgtcccatc tccgagcgcg cggtggcgat gcgcagcttg agcccctcgg
      601 gcgattcggt catgcgcgcc tccaggcgtg tgatcaactc ctcctcgctg ctggtagtca
      661 ggaagatgag caccgcctcc ggagccagct tgcgcacggt tgcagccccc tgcacgtcca
      721 ggcgcataac cacgtcctga ttgctggcca gcgcacggcg gacctgctcc ttgggcacac
      781 ctttatagtc cttgtagacc agggcatatt ccataagttc gcctcgctcg atcatcccag
      841 caaactcatc ctttgaaaag aagaaatagt ccacaccgtg cacttcgttt gggcgcggcg
      901 ggcgcgtcgt ggcagttacg acaaaataga atggcagctc gcgctgcttc atgtgctcta
      961 tgaccgtgtc cttgcctgcc cctgaagggc cagagatcac aatcaagagg ggttgatcct
     1021 gatgtgaatt aaaaatattt cgctcagtca tacaatttgc tcaggttttc cctaacctcg
     1081 cttccctgat ttaaaactcg tgggagtttc ttccctggat accaatttat ctcgacacct
     1141 gattataata ggatttacgc gattgaataa gaaagcgcct gtctggccgc gtaacttttg
     1201 tataattcaa ataaagacat tgataatgga ggtacggcat gccgttttat caatatcatt
     1261 gcctgaactg ccgcaggcga tttgatacct ttatgagcta tagcgaatac ggaaccaagt
     1321 cggtgcactg cccaaactgc aacagcgacc aggtgcgccg gcgcatcggg cgcgtgcgcg
     1381 tcgcccgctc tgaggagagt cgcatggaag acctggctga tccgggcaac ctggctggcc
     1441 tggaagatga tcctaaagcc ctggggcgca tgatgcgcaa aatgagccgc gagatgggcg
     1501 aggatatggg ccccgaattt gatgatgtga ttgaccgcct ggagtctggg caatccccag
     1561 aagaaatcga aaaagccata ccagacttag gcggagaggg cggcggggcg ggcggtctgg
     1621 acgaagattt ctaagcaaaa cgctttcctc ccctcaacac tgcactctcc attaagcgta
     1681 atctaggtct ttacaatatc atttattctc aacaggggta taaattacta tgttcctaga
     1741 gattgctcta ggacaagggg gttttctgca cctacatttt accggcaata tgttcttcaa
     1801 aatacttgcc ccaattaggt tgccgaccat cgatatcctt gaagccatac tccaatgcca
     1861 agttccagga gctgagcgcc ttgcccgaca tctgaaacac atttgggtcc gctgccagcg
     1921 cagccactgc ccgcccaatg taatacggcg tctcggaggc gatgaaatgc gggtcttttg
     1981 ttgcaccttc ctgccagttg gcctcggtta ccccaaaatg ttccagcata gcctccgagc
     2041 gaaggaagcc aggggttagg gctaaggcag tgatgttgtg cgggcgcagc tcttcagcca
     2101 tggcgtaggc gaggcggatc accgacgact tggccagatc gtagaataga ttgccccggt
     2161 aggcagcact atcgccatcc gtaatctcga tgattaaccc ccgacggcgt gcgaccatca
     2221 gcggtactgc atagcgactg gtgatgatat gcgtgtgcac accgcgctgc tgcatcagca
     2281 gccccttctg aatcgagagc tcccagaacg gctgacccca ctcggaaagc tcgtccccac
     2341 cccaaatatc gttgaccaaa atatccagct tgccttgctc gccctgcacc tgtacaaaaa
     2401 gatgttcaac ctgctcttcg tctgtatggt ccacctggac cggtatgccc actccgccat
     2461 atcttgagac caactccgca gtctcttcga ccgtttcagg acgcccttcc agggcaaatg
     2521 gggccatatt tccagatcgg cgcgatttta acctggcgcg cgtgctgcgc cccgtacaat
     2581 acaccgtggc acctgcttca cccagcatgc aggcaatgcc gcggccagcg ccgcgagttg
     2641 caccagcaac aaccgcgacc ttattggtaa gtggtttcat tttgaaaatt aataggtaaa
     2701 catgcggtcg ttctccagcg agaggcgcac caggacattg ggcatggcaa actccgccgg
     2761 cttgccggcc aggtcagctt cgttgagccc gcgcgccttg ctggacatac cggataaata
     2821 gaagcgcccg ccgcctgcaa cgatcgcatc atacgaatcc cgcaacttgc ccgttcccag
     2881 gcctgcgagc gtgtcaagca ctgcatcgcg cagcaactgg atagcatcac cggcaagaaa
     2941 cagcgtgacg gtatggccct cgtcaatcgc gctcttggcg accagaaatg caagggctgc
     3001 tcgtgtcgga tgctccggac cgtaggtgac atgaacgagt atcttagcca tgtttttcct
     3061 cctttgtctg tgagctgagg ggtagcacca atcccagcaa tacctaagtc tgactcaatt
     3121 atcctatcat tagcgaatac aaaattgaag cgctgcacag ccgatcattt ttaccagttc
     3181 accatgatca ggccagcgat acaaaccagg atacccagca ggttgatgag agataatctc
     3241 tccttgaaga agaaaagccc gatcggcacc aaaagaagta tcccagccac attgacaaaa
     3301 atggctgcga tgctgatatt ccagcccgcc cggtaggcca gtaagaaccc cacttccaag
     3361 ccgagtaggg caaaagccaa gccaaagctt gcccaattca actggctgaa agcggttctc
     3421 aagttcgagc tcaccggaaa aagaaaaaac aaaaggatac acaagatcag cgagctcatg
     3481 tacgtgacgg tcaaggccag ggctgggttc gcatcctggg gagtcatttt ttgggtcacg
     3541 tgataaagca cggttgagaa gactaataga ccacccgaga aatagtagag gaacatgttg
     3601 gaaccctttc tcattttagt taaacttacc atctgatttg cgcctggtcg cggaaaaact
     3661 gcccgcttgg gccgccatct ggcaatgtag ccagccagac gattgtgtct gcaccttctg
     3721 caacggaacg gggagcgctg gggcctttat accccaaaca agtctcgctt ttgaaaaggg
     3781 ctgaagagca tgcggcgcat gagcttgcct ttagtaagcc agcggatgca agcacctgat
     3841 ctctgattgg ctaggatttg cgcggccaac caaggcgcca cagtttcaac ccggtcggcg
     3901 agaaggttga aaacacgctt ggcgcgttcc caatcgggtg agccatcttc atattgcttg
     3961 aggatcatat cggtgatgag catgccggga cggattgaac ccacaactag cggagtgccc
     4021 tggatctcat ttactaagca ctcggtgaaa tagtgcacac catacttggt cgttccatac
     4081 aaggccaggc cattatgctt gcggccatca ctgcccatgc cttccatatt gtagaggctg
     4141 ccatagcctt gccccagcat acccttgatg gagaccatcg agccatatat gacgcccaat
     4201 agattggtct ggatgatatc ctgggcttga tcgggtggga tcttccaggc tgacagctgg
     4261 ggtccgctga agccagcgtt gttaatccat acgtccactt gcttgaagcg ctcgatagac
     4321 gcatcccaca gcgccctgat ttgctcgaga tgccgaacat cacaagcaca gccagagatg
     4381 cggctgctat catgcttctc atttagcttg ccaacagctg cagcgacgct cgactgcatc
     4441 cgaccgctaa cggtaaccga gcatccaagc tctaagaagg cgtccgccag cccaaaacct
     4501 aggccacgtg tgctgcctgt gatcacgaca tgcttcatag tttatagctg ctattctccc
     4561 ctaagaatta tagcctgtca accgccagat aacttcctgc gtttatcatg gcatcataga
     4621 aggcggggcg aagtccaccc cgcgctgaaa gttcccatcc atggaaagtt tgtaccgaaa
     4681 tccgacattc aggggcaaaa ctccgcgcaa aaccacctgg ggatgcaggt agcccaggct
     4741 cttccagagc ctgcgggcaa cccggctacg cgtgtgaaac tcgcgcagaa cccatgcaac
     4801 cccattatcg agcgtttcac gatccatctg ccgcggctca aatacgacat gattgaaatc
     4861 gtaatgactc caatccttat cgaagatccg tccctgccca tctagctcct cgaagagtgt
     4921 tgtacctggg aatggcgtca gcctggtggc ttgcaacgtc tcgatattgg cctccagtaa
     4981 aaactccagg gtttgctcaa acacgtccgg agtatcctca tccccaccaa agacaaagcc
     5041 agcgaacacc gccatccccg cctggtgtaa tctccgcacg acttccatgt agtctttccc
     5101 cagattagtg cgcttcttcc aggaacgcaa accctcctgc gacagggact caaagccgat
     5161 aaacagcccg ccacatccgc tgcgggctgc caggcgcagc agctcatcgt cggaggcaat
     5221 ccccaccccg cattggctga accatcgttt gccaagtggg atcatggcag agaataactc
     5281 tcgggcgtat tctcgatcac cgatcaggtt atcatccatg aagagcacat gaggcccaag
     5341 cgtcttgagg tcagcaatga cgtcagccac cgggcgggtg cggaaacaat actggtggaa
     5401 ggctgccacg gagcagaaac tgcagcggta gaagcagccc cgcgtggcct ggagcacatc
     5461 gaccggggca tagtccttct tgcggatgag attgcgattt agttttggca gcgcctcaag
     5521 cctgactggc tcgctgtggt agaagggctt gaggtctcca gataaaaagt cgtctatcat
     5581 gcgcggcacg ttgctctccg cctctccaat gcaaaccgcg tcggcatgct ggatagcctc
     5641 ctcgggcata aaggttgggt gatagcctcc aaatatcacg ggcttgcctt tctgggtacg
     5701 gaatatatcc gcgatctcat aggctcgcgg cgcattatag gtcatgaagg acaccccgat
     5761 gaggtccgca tccacatcaa agtcaatgga ttccacatct tcatcgataa tttgtgtctc
     5821 aacattggct ggcatcgagg ctgccacgta gaggcttggg agcatggaaa aacggaaata
     5881 gcgagagccg cgtgggcgct ctccaccgcg gacgcgccat aatggggagg ttggattgat
     5941 gagcacaaac ttaatggtct tcaaatttgg tagcctactt cttcccaccc aattacctcc
     6001 catggcatca attttgcgaa aagttgtctg gttgaggcga ttgaaagcca caccgcatca
     6061 agagcctgct tcgtaagggt cgttttcgct gcgagtaata ttggtctgaa aggaagcaca
     6121 ggtgcaggcg gggatggtct cgccatcgcg ggtcaggctg cccttataag cctgatcgcc
     6181 agcatgggcc atccaaccag agagctgaaa tgacatgggg ccatccgcgg cgatccattc
     6241 gccgttgtat ttgcgggcaa tgtggacgtg cgtgccggtt gccatccccc cttcgcagga
     6301 gggatgaccc aaatggtcgc cagcacccac ccaagtaccc accggggcgc ggctctgcgt
     6361 agaaacgtgc aaatagagca ccacccatcc agtctgctcg tagccatccc catctagatc
     6421 gaggacaacc accccgttgc cagaacgcac caccagccca gaggccgagg cggtcaccca
     6481 ggcattagac tgctggcaac ctggctcgga gctggcaggg gcaaagtcca aagcagcgta
     6541 agagccgtcg tgctcccagg caccgtgggg accgccggta tagctccaca agcggtcaac
     6601 ttcgaagggc aggatgagct cgggctgggc cagaccggga gggtaaagcg gctccactgc
     6661 ctgggcgcgc atccaagggg agccgaacat gcgctcgtac aaggcaggca aaccgtcctg
     6721 caggtcgatg gcgcgcaccc actgctcacc ggtgtagatt tgtgaaaagt aatattgcag
     6781 ggcagcgctg ccggcgttca catccggtgc caggcgggca gtgaccccat cagcgaagcg
     6841 caactccaac aaccgaccct cccgccagct atagtaaccg accgagagat ggttgattgc
     6901 ccagacaagc tgttggtaga gccctttctt gttaaggttt accaaaccca tggggtaatc
     6961 tgccattttg agatcggagg gctgaccgta gacccagccg ctctggtatt ccaacaaagc
     7021 gagcagcaag cgcgggttga tggaattctc gactgccacc cgagccacga tatcggcacc
     7081 tgtggtggtt ttggtggttt gaagccactc tttataggtg ctcagataac cacccgcctc
     7141 ttgtacatat gcctggatat caaagtcgat ggcggacggc gaataaacca cctcgctgtc
     7201 agggaatata cgctgcgaag aggccgcgtt taccagtcga cgtggaatga tcagcagctg
     7261 gttaggtggc agcagggctt cctctgggac agggtcaggg gaggtgatct cttcagggcg
     7321 cacaccaaag cgcaccgcta caacgggcaa ggtatctgca gcctgggtgt agtaaagcac
     7381 aggcgtggtg ttgatagcag gcggaacagg cgtatcctgg ggaagaggcg ggtcggtcac
     7441 cggagtggcg atatcccctg gtagagggtc cagaatcgta atttccgtgg gggtaggctc
     7501 taacaggaag gagatatcgg gtgggggcgt gtcggtgcct ttgggaggca cccagtaact
     7561 gcctgtggaa caggcaagca tagaaaaaac cagcaccaga gtcgcgacca gggaaggcaa
     7621 gcccagacga tgcctaccgc cggatagcgt tttcggccga gcgtccttca taggcctcca
     7681 cttcgtaacc atctttttgc aaatatccat cgtattccaa tcccgcgccg cgcgacaccc
     7741 agccatccag gacgaagggc aggttttggt cagcggggat ccactctcca ttataacgcc
     7801 gggcaaggtg cacatgcgtg ccagacgaga tgccaccctc gcaggaggga tgaccgatac
     7861 gctcgcctgc tttgaggaac gttcccggac ttacacgttc ccggctttcg acgtgcatgt
     7921 acagcaccac ccagccagtt tgttcatagc catccccgtc caagtcctgg atgacagccc
     7981 cattttcagc acgcaagatc aatccatcgg cgacagccgt cacccagtca ttgcttacga
     8041 tgcaccccag caggtcggaa ggcggagcaa aatccagggc agcccaggct gatccatcac
     8101 cccagccacc atgtggtcct ccggtgaaag cccaagctac ctctggctcg aagggcagct
     8161 gcatgggcgg ttgtctcaat ccaggcgaca acaatggctc aatggcgtaa tcaaaggggt
     8221 agccaaaaaa ggtgttatag acagagaaca caccattctc acccacggcc tgctcccagt
     8281 cggcgcgacc gtacaaagcg gcgaacatat gctgcacgcc agcggtgcca gcattgatgg
     8341 tggatgcgac aggcaccaca ctgccatcgc ccaacaacc
//
LOCUS       DBNGSX010000169        11743 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1536190, whole genome shotgun sequence.
ACCESSION   DBNGSX010000169 DBNGSX010000000
VERSION     DBNGSX010000169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11743)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11743)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11743
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1536190"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="AC16CB_05385"
     CDS             <1..255
                     /locus_tag="AC16CB_05385"
                     /inference="COORDINATES: protein motif:HMM:NF040290.6"
                     /note="in acetogenic organisms, this enzyme complex
                     converts carbon dioxide to acetyl-CoA while in
                     methanogenic organisms this enzyme is used to degrade
                     acetyl-CoA to form methane and carbon dioxide; part of an
                     enzyme complex; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha/beta"
                     /protein_id="HIR3341933.1"
                     /translation="MGVGKYYLISPKFISADGGFKRVVWMSSVLKESMAEELEAVAGR
                     EGDPDLINKIADDRNASTVDELLAWLEEQNHPALVMDPIF"
     gene            338..853
                     /locus_tag="AC16CB_05390"
     CDS             338..853
                     /locus_tag="AC16CB_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3341934.1"
                     /translation="MADNTQNSPRLVRELMTVGVETCPPDTLVVDLARLFLEKGIEEV
                     VVLDDGNALGVVGQEELMRAYARGGFESLKAADVLREGVLQVPPDIPLAAAAEIMLDH
                     GVRSLFLMHHAGGIEYPAAYISYRHYLRYIAAQEIDDLQDLGIYAQRSSPLETFFQKR
                     DKLRRPGSSAK"
     gene            904..1878
                     /locus_tag="AC16CB_05395"
     CDS             904..1878
                     /locus_tag="AC16CB_05395"
                     /inference="COORDINATES: protein motif:HMM:NF003376.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HIR3341935.1"
                     /translation="MPIEIPIDKWPGSVRAITIGAMQSEGGTRERTVVVGGEKAMPYM
                     HFESEMPHPPVIGMEIRDRRPDDWSPLLLETWKDVIDDPAKWAKAAEDTGVDLLVLYL
                     SLTDREGNETTPEGAVSAVKSVLGASGLPLVVFGPGQAELDNNLLVAVAEATKGERLV
                     LGICEDKNYRTIVATAMANGHLVSARTPMDVNLAKQLNILITDMGLPLERILMDPTTG
                     ALGYGIEYGFSVMERLRLAALQGDNMTQLPMIVTPGFEAWKTKESKVGEGVPASWGDW
                     LRRAINWETLTAVALLESGADIVVLRHPDSVKQVRVAIDELMAVHQIV"
     gene            1941..3260
                     /gene="acsC"
                     /locus_tag="AC16CB_05400"
     CDS             1941..3260
                     /gene="acsC"
                     /locus_tag="AC16CB_05400"
                     /inference="COORDINATES: protein motif:HMM:NF003195.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HIR3341936.1"
                     /translation="MALSGIQIYKLLPQTNCKECGFPTCLAFAMKLAAKQVELSACPY
                     VTEESKAQLAESAAPPIRLVSLKSNGYEVKAGNEVVLYRHEKTFYNRPGVFVKVQDDR
                     AIDDIKAEVARADAFTANYVGIDLTIDGIAVESVSGDATKFADAIGAVRSASQKPLIM
                     ISLNSDVMSAGLRAVDGETPLIYGASVDNWEAMAELAKQHKAALAVIADSLEGLVDLT
                     EKIKGKGVEDLVLHPASAGLSTALIMNTQIRRLALKKNFRPLGYPSMAFASKPADAAQ
                     AITKYSGFVVLDQFAPELAYPILVLRQNIYTDPQKPIQVQPGLYEINNPKTDDPVLVT
                     TNFSITYFSVANEVEGSGLPAWLLVTDAEGMSVLTAWAAGKFDSERIAKAVKTFNVAD
                     KVTRHRLVLPGHVAVLSGELESELPDWEIRVGPREAVDIPKFMKQAL"
     gene            3327..5249
                     /locus_tag="AC16CB_05405"
     CDS             3327..5249
                     /locus_tag="AC16CB_05405"
                     /inference="COORDINATES: protein motif:HMM:NF025925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HIR3341937.1"
                     /translation="MAEHLVTFDKSPNPVRVVTGTLLSEAAREAGLDIMQPCGGQGRC
                     GRCAVLVTSGSVRRRSMLRLSSEDLERGYALACQTVVEGDVSISVPPQEKIERRLTTD
                     RTVAEIEVPEGYDFARSQTVRRLTLMLTPPSMDDQTDDLSRLKIGMRQQADIGEIDIS
                     LPLLRKIGAVLREDDWRVTAILESESWDKPARSARLIDLRPGHELLNEPVWGAAIDIG
                     TTTITLWVVDLVTGKVHAQVAEYNGQISRGEDVISRIVFSAKNGGSDQLRSLVLETIN
                     ALLDRACKRVKITPDQVVKASIAGNSTMMHLLLGIPATSIRLAPFITAVNHLPLLSAH
                     EVGLAINPEGVVDCLPGIASYVGADISAGVFSSGMSNTENLTLFLDVGTNGETVLGSR
                     EWLVTCACSAGPAFEGAGVIHGMRATKGAIEEVWVNSDTFEPTFRVIGGVKPRGLCGS
                     GLISLLSEMFITGLVDKGGNINAHLGIPRVREGEHGLEYVIVWGQDSAIGEDIVITHV
                     DIDNLLRAKAAIYAGFTILADSVGIPLEAVEQVLIAGSFGKYINVEKAVQIGLLPDMP
                     WERFQFLGNTAIRGAYYALLDKEAREEIRAVASRMTYIELSADNTFYDAFMSALFLPH
                     TDISRFPSVEGVMSKT"
     gene            5330..6265
                     /locus_tag="AC16CB_05410"
     CDS             5330..6265
                     /locus_tag="AC16CB_05410"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR3341938.1"
                     /translation="MYILGENIHIISEKVKEALANKDKKFFQEQAVKQVEAGAQALDL
                     NLGPRKKDWEEVFPWIVKTVEAVVDVPLCFDSTNILGIEAGLKHVSKAQPIINSTSAE
                     PERLEKVPLLAKKYNTKLIALTIGSGGIPVAADERVTIALETLIPRMLEIDFPISDLI
                     IDPLVLTVSGCQEYCPQLIEAVRTLEYAWDPPPTISVGLSNVSNAVPHERRSLINRVY
                     CAMLMGVGLQMMIANPLEEKLKDTIRIIEERDESTPVSRLYLKIADRIQAMEEPSIDD
                     VDMDDPEQEAIWKTVQVLLNKVIYADAYLGQLEVM"
     gene            6555..7517
                     /gene="msrP"
                     /locus_tag="AC16CB_05415"
     CDS             6555..7517
                     /gene="msrP"
                     /locus_tag="AC16CB_05415"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HIR3341939.1"
                     /translation="MKKILSSEITPEQVYLNRRQFIKLGALTAGALALAACGEASDEK
                     KVPETKAEDAGETVNPQSSELKDELGDTANTYEEITNYNNYYEFSMDKEAVAPLSKDL
                     KTRPWSIEVGGLVHNPKTYAIEDILSKFPAEEHIYRLRCVEAWSMVIPWLGFPLKKLL
                     DEVEPTSQAKYVRFQTLLDSEQMPGQRGGWFDWPYVEGLRLDEAMHDLTILSTGLYGK
                     ELLPQNGAPLRLVVPWKYGFKSIKSIVRIDLVEEMPESFWMASAPDEYGFYANVNPEV
                     SHPRWSQASERRIGELGRRDTLAFNGYADEVAHLYTGMDLRANY"
     gene            7594..8238
                     /locus_tag="AC16CB_05420"
     CDS             7594..8238
                     /locus_tag="AC16CB_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016679 - oxidoreductase activity, acting
                     on diphenols and related substances as donors [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HIR3341940.1"
                     /translation="MGVDYFQDNLTINPIQELTSRTGKTALVLLVLTLAATPANTVFG
                     LRSALKVRRALGLYTFMYVSIHFLIFIGLDYGFNLKLLKEAIFEKRYALVGFAAGLIM
                     LPLALTSTRGWMARLGKNWKRLHRFVYLAGVLAVVHYVWLVKSDIRDPLAYGLAVLLL
                     LIARLRIVRKSLSSLRLNIMGRLRRQSKMIRIEINPVNSVERIRKRIFGDLNPR"
     gene            8369..9253
                     /locus_tag="AC16CB_05425"
     CDS             8369..9253
                     /locus_tag="AC16CB_05425"
                     /inference="COORDINATES: protein motif:HMM:NF046451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3341941.1"
                     /translation="MDIQKLWMVAEEAWSALAGRFEPLAEQMCAQHDMEGRTFGLLLS
                     VLTFEPEDTTPGHLMVRGPYTSADEFLNRLQGAADQGFLKEITQGKYRLSENGREITL
                     NIAEEIRRVMAEVDPLPLEDSVRLAELLDRLVQSSMNTPPPPNPWSINLSYKLIPHLN
                     PPMPFIEQAFSCLSAYRDDSHLAAWRRSGLSAIALESLTLLWRGETTSLAGLYNKLFQ
                     RGHSLGIYQGALQELGNRGLVAGSDQAIRVTRAGKGFRNNIEAYTDKYFYLPWSCLKE
                     SEMSEMLALLSRMRDGLK"
     gene            9255..11486
                     /locus_tag="AC16CB_05430"
     CDS             9255..11486
                     /locus_tag="AC16CB_05430"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR3341942.1"
                     /translation="MSIDKLIKSLPESCSPADQELVLRAYRVAERAHEGQTRASGEPY
                     VTHCVAVAAILSEFGVSAEVIAAGLLHDVVEDTSISLEDLRRDFGNEIAMLVDGVTKL
                     TQLPRVSRGDQHPEETQKEEELREIAERRGLPEPDEQEEMLTRSRRYDAASETLRKTF
                     LAMGENINVVFIKLADRLHNMRTLGKLPEIKRKRIAQQTLDIFAPLANRLGIWQMKSE
                     LEDLAFRYTNPENYGEIKDKLDTHQTQRDAEMKEITDQLENVLSKAGIQAEISSRSKH
                     IYSIYKKMVRKDLSFEMVYDVRGVRIIVPDIPACYSALGVIHTHWRPLPGQFDDYIAS
                     PKDNFYQSLHTSVVYSDGKTLEVQIRTPEMHEYAERGLGSHRIYKEQRLAQDLNYIKS
                     LMEWRFDVSDAKEFVDSMKTDVFQDRVYIFTPKGDIIDLPAGSTPIDFAYHVHTDVGN
                     RCRGAKVNGKLVSLDYALKTGDKVEVITAKRGGPSRDWLNTNLGLVKTQRARAKIRYW
                     FKRQARDQNIAQGKSILDKELRRLGLTDVNVDRLSREFDQRSLDNLYESIGCGDLPLG
                     RLVNHLTLGGKDDGEEYVISTRPVAETVPSSHEAVSVLGLKGLLTNFARCCNPAPGDD
                     IVGYITRGRGATIHRQDCPNILRVQDRERLVRVTWGEARNTYPVPVRVKAYDRDGLMK
                     DVATLIADEGVNMTGINVDVNRNLAILDLTLEVPDIAKLSRVLDRLENLPNVMAAQRV
                     KPG"
BASE COUNT         2996 a         2536 c         3140 g         3071 t
ORIGIN      
        1 atgggtgttg gcaagtatta cttgatcagc cccaagttta tctcagcgga tggtggcttc
       61 aaacgcgtgg tttggatgag cagcgtgttg aaggagagca tggcggagga gcttgaagct
      121 gtcgctgggc gtgagggcga tccagacctc atcaataaaa tcgcagatga tcgcaatgca
      181 tctacggtgg atgaactgct ggcatggttg gaagaacaaa accatccagc gctcgtaatg
      241 gatccaatat tctaaaggag atgggaataa gggataggga aacatcaatt tatcgtcgtc
      301 gcattcaccc tatccctttc ccctctaaat taaacttgtg gcagataata cacaaaattc
      361 tccccgtctg gtccgcgagt taatgactgt gggcgtggag acatgtccac cagataccct
      421 ggttgttgat ctggcgcgtc tctttttaga aaaagggatc gaagaggttg tcgttttaga
      481 tgatggcaat gctcttggtg ttgtaggtca agaggagctt atgcgcgctt acgcccgagg
      541 aggctttgag tctctcaaag ctgcggacgt gctccgtgaa ggggtgctgc aagtgcctcc
      601 agatattccg ttggcagccg cggccgagat tatgcttgac catggtgtgc gctcattgtt
      661 cttgatgcat catgccggtg gaattgaata tcctgcggct tatatttctt accgtcatta
      721 cctgcgttat atcgcagctc aagaaattga tgatctccag gatttaggaa tctatgctca
      781 gcgtagctct ccgcttgaga cattcttcca aaaacgggac aagctacgca gaccaggcag
      841 ttccgcaaaa taattaggat tgccgttaat aagtcgattc taaacgacat aggagtgaaa
      901 tacatgccga tcgaaattcc catagataaa tggcctggtt ctgttcgcgc catcacgatt
      961 ggtgcgatgc agtccgaggg ggggacaagg gagcgcacgg tggtcgtggg gggcgaaaag
     1021 gccatgcctt acatgcactt tgaaagcgaa atgccacatc ccccagtgat tggaatggaa
     1081 atccgtgacc gccgcccaga tgattggtct cccttgttat tggagacatg gaaagatgtt
     1141 atcgatgatc cagcgaagtg ggcaaaggct gctgaggata ccggggtgga tcttctcgtg
     1201 ttgtacctga gcttgactga ccgggagggt aatgagacta ccccagaggg ggctgtctct
     1261 gcagtaaaat ctgtactcgg ggctagtggt ttgccactgg tcgtttttgg cccgggtcaa
     1321 gcagaactgg acaataatct gcttgtggcg gttgcagaag cgacgaaggg cgagcgcctg
     1381 gttttaggga tttgcgagga caagaactac cgtacaatcg ttgcaacagc catggcaaat
     1441 gggcacttag ttagtgcccg cacaccgatg gatgtcaacc tggctaagca gctcaatatt
     1501 ttgatcactg atatgggctt gcccttggag cggatcttga tggatccgac tacgggcgcg
     1561 ctaggctatg gcattgaata cggtttctcc gttatggagc gcttgcgact agcagctctc
     1621 cagggagaca atatgactca actaccaatg attgttacgc ctggatttga agcctggaaa
     1681 accaaagaat ccaaagtcgg tgaaggagta ccggcttctt ggggtgactg gctccggcgg
     1741 gcgattaact gggaaacgct aactgctgtc gcattgttgg aatctggagc cgatatcgtt
     1801 gttctgcgcc atcccgattc ggtcaagcag gtaagggtag ctatcgatga gttgatggct
     1861 gttcatcaga tcgtataaca accacaattc ggtttcgatc acaacgatgg aataaagagt
     1921 agactgtacg gagattatat atggctctat ctggaattca aatctacaaa ctacttccac
     1981 aaacaaattg caaggaatgt ggattcccca cttgcttggc cttcgcgatg aaattggctg
     2041 ccaaacaagt cgaactgtca gcttgtccgt atgtcactga agagtcgaag gcgcaattag
     2101 cagagtcagc cgcaccgcct atccggctgg tttctctgaa gtccaatggc tacgaagtca
     2161 aagcgggcaa tgaagtggtc ttgtatcgtc acgaaaagac tttctataat cgccccgggg
     2221 tgtttgttaa agtccaagac gatcgcgcaa ttgatgatat caaggctgag gttgcccgag
     2281 cggatgcatt cactgctaat tatgtaggta ttgatctgac aattgatggc atcgctgtag
     2341 aatctgtctc tggcgacgcg acaaaatttg ctgatgctat cggggctgtg cgctctgcca
     2401 gccaaaaacc gctcattatg atcagcctca attctgatgt gatgtctgct ggcttgcgtg
     2461 cagtagatgg tgagacaccg ctgatttacg gcgctagtgt ggataactgg gaagcgatgg
     2521 ctgaattggc caagcaacac aaagcagccc tggcagttat cgccgattcc ttggaagggc
     2581 tagtcgactt aaccgagaag atcaagggca aaggcgtcga agacctggtt ttgcatcctg
     2641 ctagtgccgg cttgagtact gctctaatta tgaacacaca aattcgccgg ctggcgctca
     2701 agaagaactt ccgcccctta ggatatccga gcatggcatt tgcctctaag ccggcagatg
     2761 ccgcgcaagc tataaccaag tattctggtt ttgtcgtcct ggatcagttc gcgcccgaac
     2821 tggcttatcc gattttggtt ctacgccaga acatttatac cgacccacaa aagccgatcc
     2881 aggtacagcc tggcctctac gagatcaaca atccaaagac tgacgatcct gttttggtca
     2941 ctacgaactt cagcattacc tactttagcg tggcgaatga agtggagggc tctggcttac
     3001 cggcctggct tctggtgact gatgcggagg gcatgagcgt cttgacggca tgggcggctg
     3061 gcaagtttga ttcggaacga attgccaaag cggttaagac cttcaatgtg gcggataaag
     3121 tcacccgtca ccgactagtt ttgccgggac acgttgctgt gctctcgggt gagttagaaa
     3181 gcgaactgcc agattgggag atacgcgtcg gcccacggga ggcggtggat atccccaaat
     3241 tcatgaagca agccttataa aaggtgatag actgcgaccg atccacgata tatttttcgc
     3301 ttattgttgg caaatatcga gagtgaatgg ctgaacatct cgttacattt gacaagtcgc
     3361 ccaatcctgt gcgcgtagtt accggtacgc tcctttccga ggccgcgcgc gaggcagggc
     3421 tggacatcat gcaaccttgc ggagggcagg gccgctgcgg ccggtgcgca gtcttggtta
     3481 cttccgggtc tgtgcgccgc cgcagcatgc tgcgtttatc ctcagaggat cttgagcgag
     3541 gttatgccct ggcttgtcag acggttgtcg agggtgacgt atctattagc gtgccgcccc
     3601 aagaaaaaat cgagcggcgc ctcactactg atcgcacggt agcggagatt gaggtccctg
     3661 agggttatga ttttgcccga tcacagactg tccgcaggtt aacactgatg ctcactccac
     3721 ccagcatgga tgaccagact gatgatttga gtcgtctgaa aattgggatg agacaacaag
     3781 ccgatattgg cgaaattgat atctcattac ctctattgcg caagatcggg gcagtattac
     3841 gtgaagacga ttggcgggtc acggcgattt tggaatccga atcttgggat aaaccggctc
     3901 gctcggcgcg tttgatcgac ctccgacctg gacatgaact cctgaatgaa cctgtgtggg
     3961 gggctgccat tgacattgga acgaccacga tcactttatg ggttgtggat ctagtcacag
     4021 gaaaagtgca cgcacaggtt gcagagtaca atggccaaat ttcgcgcggc gaagatgtga
     4081 tttctcgtat tgtcttttcg gccaaaaacg gcggaagtga tcagttgcgc tcactggtgc
     4141 ttgaaaccat caatgcactg ttggacaggg cctgcaagcg agtcaaaata actcccgatc
     4201 aagtggtgaa ggcaagtatt gccggtaaca gcaccatgat gcacttgctt ttaggtattc
     4261 cggcaaccag catccgtttg gcgccattta ttacagctgt aaatcatttg cctttactct
     4321 ctgcgcatga ggttggcttg gcaattaatc ctgaaggagt agttgactgc cttcctggca
     4381 tcgcaagcta tgttggtgct gatattagcg cgggtgtatt ctcatcgggc atgagcaata
     4441 ccgagaatct gaccctattt ctagatgtag gcaccaatgg cgagactgtg ctcggttcaa
     4501 gagagtggct tgtgacatgt gcctgttcgg ctggaccagc ttttgagggc gctggcgtca
     4561 tacatggtat gcgagctacc aaaggggcta ttgaagaagt atgggtcaac agtgatactt
     4621 tcgagccaac gtttcgtgta atcggtggcg tgaagccacg cggactatgt ggcagtggtt
     4681 taatctcgct gctttcggag atgttcatca ccgggttagt ggacaagggc ggtaacatca
     4741 atgctcattt aggcatacca cgtgtgcgtg aaggcgagca tggactcgaa tatgtcattg
     4801 tttggggtca agatagcgcc attggagagg atatcgttat tacccacgta gatattgata
     4861 atttactgcg tgctaaggcg gctatatacg ctgggttcac aatcctggca gatagcgtag
     4921 gcatcccact tgaggcagtt gaacaagtgc tcattgccgg ctcgtttggg aaatatatca
     4981 atgtcgaaaa agccgttcag attggtctgc ttcccgatat gccttgggag cgtttccagt
     5041 ttttaggcaa tactgcaatt cggggtgctt attacgcgct gctggacaaa gaggcgcgtg
     5101 aagaaattcg ggcagtagca tccaggatga cgtatattga gctatctgct gataacactt
     5161 tttatgatgc gttcatgtcg gcattgtttt tacctcatac ggatatttcc cgctttccat
     5221 ccgttgaagg cgtgatgagt aaaacctgag ggagaacctg taatgcgcag gataataatc
     5281 ttgcgctcag atcgattaga cctgcaaaaa atcgagggag cgaaatacca tgtatatttt
     5341 aggcgaaaat atccacatca tctctgaaaa ggtcaaagaa gccctggcaa acaaggataa
     5401 aaagtttttc caggagcagg ctgtgaaaca ggttgaagcc ggtgcacaag ctctggattt
     5461 gaatctgggt ccacgcaaga aggattggga ggaagtattt ccctggatag tgaaaaccgt
     5521 cgaagctgtt gtggatgtgc cgctttgttt tgacagcacc aacatcctgg gaatcgaagc
     5581 tgggttgaag catgtcagta aagctcagcc aatcattaat tcaacgtctg cggagcctga
     5641 aaggctagaa aaagtgcctt tgctggcaaa aaaatataac accaagttga ttgccctgac
     5701 gattggctcg ggcggaatcc cggtagctgc tgatgagcga gtgactattg ctctcgagac
     5761 attgattccg cgaatgcttg agatcgattt tcctatttcc gatctgataa tcgatccctt
     5821 ggtcctgact gtatcgggat gccaggaata ctgtcctcag ttgattgagg cggtgcggac
     5881 attggagtat gcctgggatc cgccgccgac catttcggta ggcctctcca atgtttccaa
     5941 tgccgtacct cacgaacgaa ggtcattgat caaccgcgtt tactgcgcga tgttgatggg
     6001 ggttggcttg cagatgatga tagctaatcc tttagaggaa aaattgaagg acactatccg
     6061 catcatcgaa gaacgagatg aatcgacacc tgttagtcgc ctgtacttga agattgctga
     6121 tcgcatacaa gccatggaag agccttccat tgatgatgta gacatggatg atcctgagca
     6181 ggaagcaatt tggaaaaccg tacaggttct cttgaacaag gtaatctatg cggatgccta
     6241 tcttgggcaa ttagaagtga tgtaaattca ccatttatat tacgcaggag tggaaatgac
     6301 cgaggagttt tggcgggcga agcctgccca agctcctcat gtttttaatg gagcaaactg
     6361 agatacatca cccagtctag ggttgattaa ttcctggtaa cttcgcatag tcatatagaa
     6421 aattgtggta ggctgaatag ttcagccgta aataacccta aattatatta gaatattatt
     6481 atcttatttg taataaatag ttaattatta acaggtacac tattagcaca tatgaattgg
     6541 gttaggagta tacaatgaaa aaaattctgt cctctgagat cacacctgaa caggtttatc
     6601 taaatagacg ccagtttatc aagctagggg cgctcacagc cggcgcgctt gctttggcgg
     6661 cgtgtgggga ggcatcagat gagaaaaagg ttcctgaaac caaggctgag gatgccgggg
     6721 agactgtcaa cccccaatcg agtgaattga aagatgagct tggtgacaca gctaacactt
     6781 acgaggaaat tactaactat aataattatt atgaattttc catggacaag gaagctgttg
     6841 ctccattgag caaagatcta aaaactcgcc cgtggtcaat tgaggtgggg gggctggttc
     6901 acaatccgaa aacgtatgct atcgaagata ttttaagtaa atttccggct gaggaacaca
     6961 tttaccgcct gcgttgtgtt gaagcctggt ctatggtcat accctggttg ggcttccctt
     7021 taaagaaatt attggatgaa gtggagccca cttcgcaagc aaagtacgtg cgattccaga
     7081 ccctccttga ctctgagcag atgccagggc aaaggggtgg atggtttgat tggccttatg
     7141 ttgagggttt gaggctagat gaggcaatgc atgatttaac catcctgtca acaggcttgt
     7201 atgggaaaga gctattgccc caaaatggtg ccccacttcg tttagtggtt ccatggaagt
     7261 atggtttcaa gagcataaaa tcgattgtca ggattgacct ggtggaagaa atgcccgagt
     7321 cattttggat ggcttctgca ccagacgagt acggattcta tgccaatgtc aacccagaag
     7381 tctctcatcc tcgttggtcg caagcttcgg aaaggcggat tggcgaactc gggcggcggg
     7441 atacgctggc tttcaatggc tatgctgacg aagtcgcaca cctttacaca ggtatggatt
     7501 tgcgtgcgaa ctactaattt ggcaatgaga tttcctaggc ttaatttgct ccaatgggcg
     7561 gtgcacattg gggcatggat tccattaatc ctgatgggcg tagattattt tcaagataat
     7621 ctaacgatca atccaatcca ggagcttaca tcccgaacgg gcaaaaccgc cctggttcta
     7681 ttggtattaa cccttgctgc aactccggct aatacggtgt ttggtttgag atcagcatta
     7741 aaagtgcgcc gggctcttgg cctgtacaca ttcatgtatg tatcaatcca ctttctcatt
     7801 tttattggtc tggattatgg atttaatctg aaactcttga aagaagcgat ctttgagaag
     7861 cgatatgcct tggttgggtt tgctgctggc ttgataatgt tgcccttagc cttgacttcc
     7921 actcgaggtt ggatggcgcg cctgggtaag aactggaagc ggttacaccg ttttgtgtat
     7981 cttgctggtg ttttagcagt cgtgcattac gtctggttgg taaaatctga tatccgagat
     8041 cctctagcct acggcttagc tgtattactt ttacttattg cacgtttgcg aattgttagg
     8101 aaatccttga gcagcctgcg cttaaatatc atggggagat tgcgtaggca aagcaagatg
     8161 atacgtattg agattaatcc agtgaattca gttgagagaa ttcgtaaacg gatattcggt
     8221 gatctaaatc ctcggtaaat tttctagtaa attaaataca tcagacctac ttacctaagt
     8281 tgagaccggt aaatgttgcg caggcattgg gtatgatccg gtataattca ggttaggatg
     8341 atcatttaag tattggagag atagccgagt ggatatccaa aagttgtgga tggtagccga
     8401 agaggcatgg tcagcattag ctggccgatt tgagccacta gcagagcaaa tgtgcgctca
     8461 acatgacatg gaaggacgta cgtttggatt gctcttgtcg gtgttgacat ttgagcctga
     8521 ggacactacc cctgggcatt taatggtgcg cggaccatat acctcggcag atgaatttct
     8581 caaccggttg caaggcgctg ctgatcaagg tttcttgaaa gagataacgc aaggaaaata
     8641 tcgattgtcc gaaaatggtc gggaaatcac ccttaatatc gctgaagaaa ttcgccgggt
     8701 gatggccgaa gtagatccat taccgctgga agattctgta cgcttggctg agcttctgga
     8761 tcgccttgtt caatctagca tgaatacgcc gcccccaccc aatccgtggt caatcaattt
     8821 gagctacaaa ctaattcccc atttgaaccc acctatgcct tttattgagc aagctttcag
     8881 ctgcctatca gcgtatcgtg acgattcgca tctggctgct tggcggcgct cgggattaag
     8941 cgctatcgcg ctggaatcgc tgacgttgct gtggcgcggg gaaacaactt ctctggctgg
     9001 gttgtacaat aaattattcc aacgcggcca ttctttaggc atctatcagg gagcgttgca
     9061 agagctagga aaccgtggat tggttgctgg atcggatcag gcaattcggg tgactagggc
     9121 gggtaaaggt tttcgcaata acattgaagc ttacacggac aaatactttt atctaccctg
     9181 gtcttgtcta aaagagtccg aaatgagtga gatgttggct ttattatcca ggatgaggga
     9241 tggcttgaag taacatgagc atcgataaat tgatcaaatc actgcccgag tcctgttcgc
     9301 cggctgacca ggagctggtg ttgcgggcat atcgcgttgc agagcgcgct cacgaggggc
     9361 aaacgcgcgc ttctggagaa ccctatgtga ctcattgcgt ggctgtggca gcaatacttt
     9421 ccgagtttgg cgtttcagcc gaagtcattg ctgcgggttt gttgcatgat gttgttgagg
     9481 acacctcaat ctccctggag gacttgcggc gcgatttcgg caatgaaatt gctatgttgg
     9541 tcgacggggt taccaagctt acccaattac cgcgcgtctc ccgcggggat cagcaccccg
     9601 aggagacaca aaaagaagag gaattgcgtg agattgctga gcgccgcggt ttgcccgaac
     9661 cggatgaaca agaggagatg ttgacccgaa gccggcggta cgatgcggct tccgagactc
     9721 ttagaaaaac cttccttgcg atgggggaaa atataaatgt tgtgtttatc aagctagccg
     9781 atcgactgca caatatgcgc acgttgggca agctgccaga aattaaacgc aaacgtattg
     9841 cccaacaaac attggatatc ttcgcgccgc ttgccaatcg cttgggtata tggcagatga
     9901 aatcagagct tgaagatctt gccttccgct acacaaaccc tgagaattat ggggaaatca
     9961 aggacaaact tgacactcat cagactcagc gggatgctga gatgaaggag attaccgacc
    10021 agcttgaaaa tgttctctct aaggcaggta ttcaggctga gatttcttcc cggtcgaaac
    10081 atatttattc catctataaa aaaatggtac gtaaagattt aagctttgag atggtttatg
    10141 atgtgcgagg cgtgcgaata attgttccgg atattcctgc gtgttattct gcacttggag
    10201 tgatacatac tcactggaga ccacttccag gccagttcga tgattatatt gcatccccga
    10261 aagacaattt ctaccagtcc ctgcacacct ccgttgtata ttcagatggt aaaaccctgg
    10321 aagttcagat acggacaccg gagatgcatg agtacgctga aaggggcctt ggatctcata
    10381 ggatttataa agagcagaga cttgcacagg accttaatta tataaaatct ttgatggaat
    10441 ggcgttttga tgtttcagat gccaaagaat ttgtagacag catgaagacg gatgtcttcc
    10501 aggaccgcgt gtatattttc accccgaaag gggacattat cgacttaccc gctggttcta
    10561 cacccattga ttttgcctac catgtccaca cggatgtggg taatcgatgc cggggtgcga
    10621 aggtaaatgg aaagctagtc tcattggatt atgcgctgaa aacaggtgat aaagttgaag
    10681 tcattacggc taagcgggga ggcccaagtc gtgattggct gaataccaat cttggtttgg
    10741 tgaagaccca acgggcacgg gccaaaatcc gctactggtt caagcggcaa gcacgcgatc
    10801 aaaatattgc ccagggtaaa tccattttgg acaaagagct gcgtcgactg gggttgaccg
    10861 atgtaaacgt tgatcgtctg tcgcgcgagt tcgatcaacg cagcctggat aatttgtatg
    10921 aatccatcgg atgtggagat cttccgcttg ggcgtttggt caatcattta actttaggag
    10981 ggaaggatga tggcgaagag tatgtcattt caactcgtcc agttgcagaa actgtccctt
    11041 catctcatga ggctgtcagt gttttaggat tgaagggctt gctgaccaat ttcgcacggt
    11101 gttgtaaccc agctccaggt gatgatattg tgggttacat caccagaggg cgaggggcga
    11161 cgattcacag gcaggattgc ccaaatatct tgcgcgtcca agatcgggag cggctagttc
    11221 gtgttacctg gggtgaagcg aggaatactt acccggtgcc agtgagagtg aaagcctatg
    11281 accgtgatgg cttgatgaag gatgtggcta cattgattgc cgatgaaggg gtcaatatga
    11341 ccggcataaa tgtagatgta aacaggaatc tggcaatttt ggatttaact ctcgaggttc
    11401 ccgacatagc caaattgagc cgggtactcg atcgcctgga aaatttaccc aatgtgatgg
    11461 ctgcccagcg tgtcaaacct gggtaaaata tcacgaagga tatcaatttg cgtgatgtgc
    11521 ttacccttta tgtgccttgg gggctagcaa tggggcgcag tgcaaattag cctaaatctg
    11581 ataaatcatt aaaaaagcct tctattttgc tctctacaat ctcgtggata attgatgagt
    11641 ttgcctaata aaaatgtttg tgtttattaa ggcacaatag ttcaattttt attcaaggga
    11701 atggtataat tttgcgccgc gcccattcga tgaatgtgcg taa
//
LOCUS       DBNGSX010000170         2027 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1397782, whole genome shotgun sequence.
ACCESSION   DBNGSX010000170 DBNGSX010000000
VERSION     DBNGSX010000170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1397782"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="AC16CB_05435"
     CDS             <1..1017
                     /locus_tag="AC16CB_05435"
                     /inference="COORDINATES: protein motif:HMM:NF024634.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HIR3341943.1"
                     /translation="NIVLATGFDLFDARRVSNYGYGRLANVFTSLEFERLSNAAGPTN
                     GNVVLRDGVTVPKAVGIIHCVGSRDRNFNNYCSVICCMQGLKFAHLIHERTGATVYNF
                     YIDMRTAFKAYDEFYQRILEEGTLFVRGKVAEVTDAARLPGEEGKLIIQVEDTLAGKQ
                     RRIPVDMVILSSGLEPRHDSKEVAKLFGISCSADGWFIEKHPKLDPVATMTEGVFIAG
                     CVQGPKDIPASVSQGAAAAARVLGRIQQGEIALEPVRATVNENKCSGCRICNDLCPFN
                     AILFHEDRMVTEVNPALCQGCGTCIAACPAGAIAGTGFSNEQILSQIEGLLLLNLAPE
                     LVPA"
     gene            1083..1556
                     /locus_tag="AC16CB_05440"
     CDS             1083..1556
                     /locus_tag="AC16CB_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HIR3341944.1"
                     /translation="MNDNHIFEPVIIGFTCNWCSYRAADLAGTARMKYPPNVRLIRLM
                     CSGRLDPTFVIKAFSGGADGVMITGCHPGECHYIEQNYKALRRYLLLRRTLAQFGIEP
                     ERIKLVWASAAEGARLSEEITKMVEDIRTLGPLNWPKLSNGRPDHTVLLQEEVKV"
     gene            1553..>2027
                     /locus_tag="AC16CB_05445"
     CDS             1553..>2027
                     /locus_tag="AC16CB_05445"
                     /inference="COORDINATES: protein motif:HMM:NF013242.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HIR3341945.1"
                     /translation="MTTNGKPKFAMYWAASCGGCEIAVLNIHEKILDVDANFEVAFWP
                     VAMDAKYKDVQAMPDKSILLTLFNGGIRNNEHEEIAHLMRRKSQILVAFGSCACEGCI
                     PGLANFSQTEQIFEAAYQSISTDNPDRILPQPDYSMPEGEIHIPEFYPVLKTLDQV"
BASE COUNT          498 a          516 c          519 g          494 t
ORIGIN      
        1 aatatcgtgc ttgcaaccgg ctttgacctg ttcgatgctc ggagagtctc taattatggt
       61 tatggacgac tcgctaacgt ttttacaagt ctggagttcg aacgcctgtc taacgctgct
      121 ggccccacta acgggaatgt ggttctccgg gatggtgtaa ctgtccccaa agctgttggt
      181 attatccact gcgtgggcag tcgcgaccgt aacttcaaca actattgctc ggtcatttgt
      241 tgtatgcagg ggttgaaatt cgcccatctc attcatgagc gcaccggtgc aacagtttat
      301 aacttctata ttgatatgcg caccgcattc aaagcttacg acgagtttta ccagcggata
      361 ctcgaagaag gaacactctt tgtgcgcggt aaggtcgccg aggtgacgga tgcagctcgc
      421 ttacccggcg aagaaggcaa actgatcatc caggttgaag acactctcgc tggtaagcag
      481 cgacgtattc cggtagatat ggtcatcctc tcatctggac ttgagccacg ccacgactca
      541 aaggaagtcg ccaagctgtt tggtatctcc tgcagcgctg atggctggtt tatcgaaaag
      601 caccccaaac ttgacccggt cgcaaccatg acagaaggcg tatttatcgc cggttgcgtg
      661 caaggtccaa aggatatccc tgccagcgtc tcccaaggag cagcggcggc ggcgcgcgtc
      721 ctgggtcgta tccaacaagg cgaaattgct ctcgaacctg tacgtgctac cgttaacgaa
      781 aacaagtgct ctggctgccg gatttgcaac gatctgtgcc cgttcaatgc gatccttttc
      841 cacgaagatc gtatggtgac tgaagtcaat cctgctctct gccagggatg cgggacttgc
      901 atagccgctt gccctgctgg agctatcgct ggaactggat tcagtaatga acagattctc
      961 tcccagatcg agggtttgct gctgctcaat ttagcgccag aactcgttcc tgcttgaagg
     1021 gcgaaccatg aagcaattca acccaatcca acaaagcgtc atcttccaat gggagatcaa
     1081 ccatgaatga taatcatatt tttgaacccg tgataattgg gtttacctgt aactggtgca
     1141 gctatcgcgc cgcagacttg gctggcactg cacgcatgaa atatccacca aatgtccgtc
     1201 tgattcgtct gatgtgctca ggtcggttag atccaacttt tgtgataaaa gcgttctcgg
     1261 gcggagccga tggggtcatg attacgggat gccatccggg tgaatgtcat tacatcgaac
     1321 agaactacaa agctttacgg cgttacttac tcttacgccg cacccttgct caatttggca
     1381 ttgaaccgga gcgcattaaa cttgtatggg caagtgcagc tgagggggct aggctatccg
     1441 aggaaatcac caaaatggta gaggatattc gcactcttgg tccactgaac tggccaaaac
     1501 tgtccaacgg acgccctgac cacaccgtgt tgctgcagga ggaggtaaaa gtatgactac
     1561 aaatggcaag ccgaaattcg cgatgtactg ggctgcctcc tgcgggggtt gcgagatcgc
     1621 tgtgctcaat atccacgaga agattttgga tgtagatgct aacttcgagg tcgcgttctg
     1681 gcctgtagcc atggatgcca agtacaaaga tgtgcaggcc atgcccgata agtccatttt
     1741 gctcactcta ttcaatggtg gcattcgcaa caacgaacac gaggaaattg ctcacctgat
     1801 gagacgcaag tcgcagatcc ttgtcgcctt cggatcctgc gcatgtgaag gttgcatacc
     1861 gggtttggcg aactttagcc aaacggagca aatatttgag gcagcatacc agagcatttc
     1921 aaccgataac ccagacagaa tcctgcctca acccgactat tcaatgcctg aaggcgagat
     1981 tcacatcccg gagttctatc cggtgctaaa aacactcgat caggtgg
//
LOCUS       DBNGSX010000171         6110 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_289710, whole genome shotgun sequence.
ACCESSION   DBNGSX010000171 DBNGSX010000000
VERSION     DBNGSX010000171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6110)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_289710"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="AC16CB_05450"
     CDS             <1..225
                     /locus_tag="AC16CB_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016997795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR3341946.1"
                     /translation="LSDPAFDTADFNDSAPGNLRADYVLPRKNLRIVDAGVFWPLNTD
                     PLFSLVGTFPFPSSDHRLVWVDAWVPGSQN"
     gene            476..1375
                     /gene="ppk2"
                     /locus_tag="AC16CB_05455"
     CDS             476..1375
                     /gene="ppk2"
                     /locus_tag="AC16CB_05455"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009148897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HIR3341947.1"
                     /translation="MAKKNKNDKGKENLREKPPKQSEPIAAPLVVEAEPAKELEKKLR
                     NKEYLKELGQLQIELVKLQEWIKLKGLKLVVIFEGRDAAGKGGVIKRITECLNPRYAR
                     VVALPAPTEREKTQWYFQRYVAHLPAAGEMVFFDRSWYNRAGVERVMGFCTNEEYQEF
                     LRACPEFERMLVRSGIILVKYWFSVSDEEQERRFQARIEDPTKRWKLSPMDLESRTKW
                     VEYSIAKDEMFKYTDIKQAPWYVVNSDNKKRARLNCISHLLSVIPYQDLTPEPIVLPP
                     RREDKTGYVRPPITDQTFVPEDY"
     gene            1705..2592
                     /locus_tag="AC16CB_05460"
     CDS             1705..2592
                     /locus_tag="AC16CB_05460"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012958.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HIR3341948.1"
                     /translation="MKGAPDVQGKTAEAITSPQSPPTQMEKATPTNSQPMPTETPTPT
                     KSQPSTVETVTPTASLPTPVAPFTPGSIGPGEQMAYSFQSSSGEEIIFWLYLPQGYDE
                     SRSWPLILSLHGFLGFEPSLEKVRGQSPLAWVSPDVEFPFIVIAPQAPSGSWAKYHEP
                     MDELIEYLGESLSIDPQAQFLTGLSAGAIGAWQWALAFPDRFAGVAPIAGGLSMNRND
                     PVPENICSLKDLPIWAAHSEADREAPIDSVLAAVSALEECGSTVIRFNVYTDLSHIDT
                     ISTAYAGPELYDWMLGLVR"
     gene            2597..3055
                     /locus_tag="AC16CB_05465"
     CDS             2597..3055
                     /locus_tag="AC16CB_05465"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3341949.1"
                     /translation="MIPGEHEFTLNIRQAQIDDIPFIVQLIQELAEMEKVSSPLNKTY
                     VEYYLSQPNCYILVAEAGDQSIGLLSYMMKPDLFHGSDTCTISELVVSKGFRGRGVGS
                     ALLDHLMRQLISIGCVEISVSTLPDNQGAIQFYKRHGLVDEAVLLERHFN"
     gene            3156..3953
                     /locus_tag="AC16CB_05470"
     CDS             3156..3953
                     /locus_tag="AC16CB_05470"
                     /inference="COORDINATES: protein motif:HMM:NF019322.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3341950.1"
                     /translation="MTSLSSDLFAIFQTDSPQQVSLPEFTANRIKEMINSRKLLPGAR
                     LPNELELADAMGVSRGTMRTALNILQEQGLIWRRQGIGSFVSEQPILENRLDINSGVT
                     ELIESMGLTPGTKILEIKQVAADVHQAGQLNLPIGSQLVFIRRIRMANTRPVVSSIDI
                     FPYEILKQGTGLMELEKFKMLLEKGHSIYRLFESEFSLSIDYGITRLRPVKVNARQMR
                     HIGLGLPAGSVLLYMEQLDYDRNRRPIYFSSEYHVADFCTFTIFRRR"
     gene            4062..5606
                     /locus_tag="AC16CB_05475"
     CDS             4062..5606
                     /locus_tag="AC16CB_05475"
                     /inference="COORDINATES: protein motif:HMM:NF013697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3341951.1"
                     /translation="MKSKLYIVLSILVSAVLLLSACAPAPAPTPEAPEPPAGGEAPAG
                     EWTLATAAEPWKGETLRLIGEALPPLEALTQLKGEFEEITGVNVVIEGLGHEEAVEKV
                     TLDFAGQTGVYDVVLQPHRALGKLVANGWVRPIEEFYNDPALRNPDFSFENDLLNQTW
                     FRETCTYQGDIYALPFHFISMYLWYRYDWFEHPDEQAAFKAEYGYDLPSPPVTWQEYR
                     DAAEFFTRASGETLAGETLDHDVYGHAWGAARHVSTWYDYLNVLYSVGGRDIQLPDNR
                     GDSYGPVTINSPEALEALNMIIDMMQFAPPGAVSYTWDEKQAAQQSSLVAMAIQWDDA
                     TFAVEDPEQSTASGKIAYSGVPIGKEKMTQIEGWSYFIPNSSKKPELAWLFIQWAMGK
                     DVQIAQQNLGGESALRPTYDDAQVSSIPYAPTAVYLKTAGEWVLKERNIGDATGLGVP
                     KRYLEAVNPATGDTSVSIVAKPTFPEEERLTEAIILHVNRAVSGEVTPEEALSDLAEE
                     MESILP"
     gene            5913..>6110
                     /locus_tag="AC16CB_05480"
     CDS             5913..>6110
                     /locus_tag="AC16CB_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3341952.1"
                     /translation="MTTLTLKNVWKIYEGNVLAVKDVTLSINEGEFFALLGPSGCGKS
                     STLRMIAGLEKLTKGEIWFDHT"
BASE COUNT         1563 a         1624 c         1498 g         1425 t
ORIGIN      
        1 ctgagcgatc cagccttcga tacggctgac tttaacgatt cggcacctgg taacctgcgc
       61 gccgattacg tgctgccgcg caagaacctg cgcatcgtag acgccggcgt cttctggcct
      121 cttaataccg acccgctctt ctcgctagtc ggcaccttcc ccttccccag ctccgaccac
      181 cggttggtgt gggtggacgc ttgggtgccc ggttcccaaa actaacccta cctcaattgc
      241 aaccttgcgc ctggcacaat aagaatgcca ggcgcaattt gattaagtgt tacgcgtcca
      301 cacggagagt gacccattaa aaccaacaac caatcccggt tttttctgcc tgattttcga
      361 caatgtccaa atctcgtcca agcatgagcc gctccggttg acaaaggggg aatctcccct
      421 ataataaatg gcagctaaca atcgtcaatc cttataattt tctcgggagg caacgatggc
      481 aaaaaagaat aaaaacgata aagggaagga aaatctgagg gaaaagccac ccaagcaatc
      541 tgaacccatc gccgcgcctc tcgttgttga agccgagccc gccaaggaac ttgagaaaaa
      601 actgcgcaat aaagaatatc tcaaagaact cggtcaatta cagatcgagc tggtcaaatt
      661 gcaggaatgg atcaagctca aaggcttaaa gctggtggtc atctttgaag gccgcgacgc
      721 ggctggcaag ggtggcgtga tcaagcgcat caccgagtgc ctgaaccctc gctatgcccg
      781 cgtggtggca ttgcccgccc ctacagagcg cgagaaaacg caatggtact tccagcgtta
      841 cgtggcgcat ctcccggcgg ctggcgagat ggtcttcttt gaccgcagct ggtacaaccg
      901 ggctggcgtt gagcgcgtca tgggcttttg caccaatgaa gaataccagg aatttctgcg
      961 cgcctgcccc gagtttgagc gcatgctggt gcgctcaggg atcatcctgg tcaaatactg
     1021 gttctcggtc agcgatgaag agcaggagcg ccgcttccag gcgcgtatcg aagaccccac
     1081 caagcgctgg aagctcagcc caatggacct cgaatcgcgc acaaagtggg tagaatactc
     1141 catcgccaag gatgagatgt tcaagtacac cgatatcaag caggcaccct ggtacgtggt
     1201 caactccgac aacaagaagc gcgcccgcct gaactgcatc agccacctgc tgagcgtcat
     1261 cccctatcaa gacttgacgc ccgagccgat tgtccttccc ccgcgacgag aagataagac
     1321 cggatacgtg cgcccgccca tcaccgacca gacttttgtt ccggaagatt attaagtaac
     1381 attacgcgac atcgatcatc ccgcttatta gttgggatac gcatccagaa caccaatcac
     1441 attcaactac tcgttagcaa taacggcata aaatctttcc atacctggat acacactgct
     1501 ggcctgcaac ggttgtgctc ggattcggta gattgttcag gcagtaaatg aaagaagtca
     1561 ggtatgctga agagggaaac gaacctcaaa ttttgttcac tgtccaaagg actgaaatcg
     1621 gaaggtaggc accaggagga tcaaatgaaa cggatattta tttattcggt ggtgattttt
     1681 ttcttactat caggatgttc cccgatgaaa ggtgcccctg atgtgcaagg taaaactgcg
     1741 gaagccatta catcgccaca atccccgccc actcaaatgg aaaaagccac tcctacgaac
     1801 tctcagccta tgcctacaga aacccctact ccgacaaaat cccagcctag cacagtcgaa
     1861 accgtcactc caaccgcatc gctacccacc cccgtggccc catttacccc aggctcgata
     1921 ggacccggcg agcagatggc ttattccttc cagtccagct cgggcgaaga aataattttt
     1981 tggctctatt taccgcaggg ttacgatgaa agtcgaagtt ggccactcat tctttccctc
     2041 cacgggttcc tcggctttga gccgagccta gaaaaagtgc gcgggcaaag cccgctggcc
     2101 tgggtcagcc cggatgttga gttccccttc atcgttatcg cccctcaagc tccgtctggc
     2161 tcgtgggcga aatatcatga gccaatggat gaattaattg agtatttggg cgaatcttta
     2221 tcaatcgatc cgcaggctca atttctcact gggctaagcg ccggcgcgat tggggcttgg
     2281 cagtgggcgc ttgcatttcc ggatcgtttt gctggggtag ccccgatcgc cggtggtcta
     2341 tcaatgaatc gcaatgaccc ggtcccggaa aatatctgct ccctcaagga tctgcctatt
     2401 tgggctgctc acagcgaagc cgatcgagag gcacccattg attctgttct cgcagcagtt
     2461 tcagctttgg aagagtgcgg gagcactgtt attcgtttta atgtatatac agacttgagc
     2521 catatagaca ccataagtac agcctatgca ggtccagagc tctacgattg gatgctgggg
     2581 ttggtgagat aagtaaatga ttccaggaga acacgaattc actctcaata ttcgccaggc
     2641 gcagatagac gacatcccgt tcatcgtcca gttgattcaa gaactggctg agatggagaa
     2701 agtatctagc ccactcaaca agacttacgt cgaatattac ctttcacagc ccaattgcta
     2761 tatcctcgtg gctgaagctg gggatcaatc catcgggttg ctcagctaca tgatgaaacc
     2821 cgacctcttt cacggcagcg atacctgtac tatttccgag ctagtcgtct caaaaggttt
     2881 tcgcggtcgc ggagtgggca gcgccttgct tgaccacctg atgcgacagc taatttctat
     2941 cggctgtgtt gaaatctccg tctcaacttt gccagataac caaggggcaa tccagtttta
     3001 taagcgtcat ggattggtcg atgaagccgt cttattagag cgccatttca attaaggatt
     3061 aaccgtaaat atattataaa ttcttcttga cataggatat atttttctat acactaatac
     3121 caatcattaa tcattaaaca ttaatcatta tatttatgac ttccctgagc agcgatttgt
     3181 ttgccatttt tcagacagac tctccccagc aagtcagtct gcctgagttc actgcgaatc
     3241 gcatcaaaga aatgatcaac agccgcaaac tcctgccggg ggcacgatta cccaacgagc
     3301 ttgagctggc agatgcgatg ggggtcagcc gcggcacaat gcgcactgcg ctaaacatcc
     3361 tccaagaaca aggcttaatc tggagacggc aaggaattgg tagctttgtc agcgagcagc
     3421 ccattctcga gaacaggctg gacattaata gcggtgtcac tgaactgatc gaatcaatgg
     3481 gcctgacacc tggcacaaaa attctggaaa ttaaacaggt agccgctgac gtccaccaag
     3541 ctgggcaact caacctgcca attggtagcc aattggtttt tatccgccgc atccgcatgg
     3601 cgaatacccg accggtagtc tcatcgatcg atatcttccc atacgaaatt ctcaagcagg
     3661 gcacaggctt gatggagtta gaaaaattta aaatgctcct tgaaaaaggc cactctatct
     3721 atcgactatt cgagagcgag ttttcgctat ctatagacta cggtatcacc cgcttgcgcc
     3781 ctgttaaggt taacgcccgc caaatgagac acattggtct tggattgcca gcgggttccg
     3841 tgctgctcta tatggagcaa ttggattacg accgcaaccg cagaccaatc tacttttcca
     3901 gcgagtacca tgtcgccgat ttctgcacct tcacgatctt ccggcgcaga tgaaataatg
     3961 atttgacaga aaggaggcct ttggagacca gagattcaac gtttctatcc gattcgttta
     4021 tctaatccca aacaaacata aagaagaaga ggaggagtag catgaagtcg aaattgtata
     4081 ttgtgcttag cattctcgtc agtgctgtgt tattgctctc agcttgtgca ccagcgccgg
     4141 caccgactcc tgaggcccca gaacctccag cgggtggtga agcgcccgct ggtgaatgga
     4201 ccctcgccac agccgctgaa ccctggaaag gagaaaccct gcggctgatc ggcgaagccc
     4261 tgccccctct ggaagccttg acgcaattga agggcgagtt tgaggagatc acaggggtta
     4321 atgtcgtcat cgaaggcctg ggacatgaag aggcagtcga gaaagtcacc ctggattttg
     4381 ccggtcaaac cggcgtatac gacgtggttt tgcagccaca ccgtgctctc ggaaagctcg
     4441 tagccaatgg gtgggtacgc ccaatcgaag aattctataa cgacccagcc cttcgcaacc
     4501 cggacttcag tttcgagaat gatctactca accagacatg gttccgtgaa acctgcacat
     4561 accagggtga catctacgct ttgccgttcc atttcatcag catgtacctg tggtatcgct
     4621 atgactggtt tgagcaccca gacgagcagg ctgcctttaa agctgagtac gggtacgacc
     4681 tgccgtcgcc tccagttaca tggcaagaat atcgcgatgc agccgagttc tttacccgcg
     4741 cgagcggaga aaccctggct ggcgaaaccc tcgaccacga cgtctatggt catgcttggg
     4801 gcgcggctcg ccatgtatct acttggtacg actatttgaa tgttctttac tctgtgggtg
     4861 gtcgggatat ccaactgcct gacaaccgtg gcgacagcta cggccctgtg acaataaatt
     4921 cccctgaagc tctagaagcg ctcaatatga tcatcgacat gatgcagttc gcgccacctg
     4981 gagctgtcag ctatacctgg gatgagaaac aggctgctca acaatccagc ctggtcgcaa
     5041 tggctattca atgggatgat gccactttcg cagtcgagga cccagagcag tcaactgcct
     5101 ccggcaagat tgcctatagc ggtgtcccca tcgggaaaga aaagatgacc cagattgaag
     5161 ggtggagcta cttcattccg aacagctcaa agaaaccaga gttagcctgg ctattcatcc
     5221 aatgggcaat gggcaaggac gtgcaaatcg cccagcaaaa cctgggcggc gagtccgcgt
     5281 tgcgaccaac ctatgatgat gctcaagtat cctccatacc ttacgccccc accgcggtct
     5341 acttgaagac tgctggagag tgggtgctca aggagcgcaa tattggagac gccactggtc
     5401 tcggtgttcc aaagcgatac ctggaagcag ttaatccagc aaccggcgat accagcgttt
     5461 ccatcgtcgc taagcccacc ttcccggagg aggaacgctt aaccgaagcg attattctgc
     5521 atgtaaatcg cgctgtcagt ggcgaagtca ccccagaaga ggcgctcagt gacttggcgg
     5581 aagaaatgga gagtatcctt ccataggcat gcatcccctc gcattcttgt ggagatttga
     5641 attggctggt tttgcggctt ttcagccgca aaaccagcct ccatactgca atacaggaga
     5701 tcactcatga atattgggga caagctcacc tgggcttatc gatcactgtt cattttgatg
     5761 ctaatatggc tgcgccttat tgaaacttat attcccttct ggggtatctg gatcgtgtgg
     5821 gggatcattg tctttataat tttttaccaa ccgttcaaaa gaaaacagct ccaaccaaag
     5881 cctcaggagg aagttagaca agaggcgaaa ccatgaccac gttaaccctt aagaacgtgt
     5941 ggaaaattta tgagggtaac gtccttgccg ttaaggatgt taccctctcg attaatgaag
     6001 gggaattctt cgctctgctc ggtccttccg ggtgtggaaa atcatccaca cttcgcatga
     6061 tcgcaggctt ggaaaagtta acgaagggtg aaatttggtt tgaccatacc
//
LOCUS       DBNGSX010000172         4002 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1744333, whole genome shotgun sequence.
ACCESSION   DBNGSX010000172 DBNGSX010000000
VERSION     DBNGSX010000172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1744333"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="AC16CB_05485"
     CDS             complement(<1..230)
                     /locus_tag="AC16CB_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341953.1"
                     /translation="MRILLLLVILVVWWIFWHTDTVFSHILHARPDNAVTVRMLIPWP
                     TAFVWISWGVTFWFLAWIAVTFAPKLKRQKLR"
     gene            complement(249..1868)
                     /locus_tag="AC16CB_05490"
     CDS             complement(249..1868)
                     /locus_tag="AC16CB_05490"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3341954.1"
                     /translation="MFHLNTWVKVAGMVFLILLTVHPASTTLAQSRQTVAFVNVNVIP
                     MDTERVLESQTVIVQGDRIVEIGAAKEVSVPPGAQVIDGNAKYLIPGLTDTHFHILDN
                     QDSLILAIANGVTTIRDPNADYVGTGMEILEARDQIAAGQRLGPTIMAAKSIIGASDR
                     VQVLFDNVDKAVGPWFAGDWGAATNIPTDPATGRALVYKAHEEGYDIIKVNWYLSRET
                     FDSIVAAAAELGMPVLAHVPADVGIEHAIRSGVEIQHNGNLLAFYAKDYVRQPGPNYL
                     DAFDLSEADQRLPELVTLMKENDVAFTPTMAVDVTAFELFDNLPDWSRADIFQHPGYR
                     YTPPSYMSQWTNAAGGEFGVVARGRGVSSVEEIVPPPDAREEILALHLRQLKAFVDAE
                     VPVMTGSDASAVGVVWGFSIHEELELFVKAGLTPYQALEASTRIPSEVMGDPEEWGTI
                     KVGKRADMVLLNANPLENISNTREIEGVIVRGQWLSKDMLQSMLNELAATYEIPKKVQ
                     LMFASVLTLGALIFVVFIVRGVRNGRRRLAW"
     gene            complement(1923..2588)
                     /locus_tag="AC16CB_05495"
     CDS             complement(1923..2588)
                     /locus_tag="AC16CB_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341955.1"
                     /translation="MKIKRFLHKIFVLIILLAALFVLAACSGKGQAKEPSFPSTEFVS
                     STKLFSLYVPTGWSAHEVIPGANLVMASTEAALDRYRNRNALESGELVLNVGFLPLAL
                     FQEKELSHLGIQSDSSPDAFLQSLLPMFVVGDEPARNVAGETALVTLGDGRDAGMLTF
                     SKEGREGMTLVFRAGKDVFAFISAATYPGGLSESQEIIYALATGVTYNGSQDALYQKL
                     YGD"
     gene            complement(2620..2898)
                     /locus_tag="AC16CB_05500"
     CDS             complement(2620..2898)
                     /locus_tag="AC16CB_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341956.1"
                     /translation="MDPLPEFNKPAYYEFRVRSRISPQSAAWFEGMEIAVDETAEPVQ
                     TLIKGYMEDQSALHGLISRIRDLGLTLVSVNHIERKEDQNQNDGASEV"
     gene            complement(2999..3802)
                     /locus_tag="AC16CB_05505"
     CDS             complement(2999..3802)
                     /locus_tag="AC16CB_05505"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR3341957.1"
                     /translation="MKSFIERHPFWFSLLFTIIVMQILGFVVAILGRVLGLPDLVIRT
                     SAMGVIAIVSLIFIWQIGWWKDAGLVNTTQNVYALAVPLILTFFPLVFFGTYTMEPQR
                     VNIFLLAVLFTGISEEAVFRGLFIRAFLPHGKLKAVLIPAILFGAAHIVQSLGGGMSL
                     EDNLVQILNAFLYGVMLGAVRLRVNNIWPLIILHTVHDLFWVTAGLPDGVITMAEIPL
                     SEYLIEWIPSVVTAIYLMRKPIAVTINGVPVGTMDKPLAASRAVSQPAD"
BASE COUNT         1025 a         1054 c          965 g          958 t
ORIGIN      
        1 tgtctcaatt tttgtctttt gagctttggt gcaaaggtga cggcaatcca agccaagaac
       61 cagaacgtta ccccccagga aatccacaca aaagcggtcg gccaaggaat cagcatgcgg
      121 acagtgacag cattgtcagg acgagcatgc agtatgtgag aaaacacggt atccgtgtgc
      181 caaaagatcc accatacaac caagatcacc aaaagcaata agatacgcac aagcttgcct
      241 cgggacaact accaagcaag tcggcgacgt ccattgcgga ctcctcgtac aatgaaaaca
      301 acaaatatta gcgcaccaag cgtcaataca ctcgcaaaca tcaattgcac tttttttgga
      361 atttcgtatg tggcagccaa ctcattcaac atgctctgaa gcatatcttt ggacaaccac
      421 tgccctcgca ctatcacacc ttcaatttca cgggtattgc tgatgttttc cagggggttg
      481 gcattcaata gcaccatatc agcccgcttg cccactttga tcgttcccca ttcctctgga
      541 tcgcccatca cctccgatgg aattcgagtt gacgcctcca gcgcctgata tggcgtcaaa
      601 ccggctttga cgaagagttc gagctcctcg tggatcgaga atccccatac cacgcccaca
      661 gcgcttgcgt ctgaaccggt catcacgggt acctcggcat cgacaaatgc tttgagctgt
      721 cgaaggtgca gtgccagaat ttcctcccgg gcatccggtg gaggcactat ttcctcaacg
      781 ctggaaacac ctctaccgcg ggctaccacg ccaaattcac caccagcggc attcgtccat
      841 tgggacatgt aagaaggagg agtgtatcgg tatcccgggt gctgaaagat gtccgctcga
      901 ctccaatcag gcaggttatc gaagagttca aaggcagtaa cgtcaaccgc catggttggc
      961 gtgaaggcaa catcgttttc tttcatcaag gttacgagtt ccggcagtct ttgatctgcc
     1021 tctgacaaat cgaacgcatc gaggtaattg ggtccgggtt ggcgcacata atccttggca
     1081 taaaaagcca gtaggttacc gttgtgctgg atttccaccc cagaccgaat cgcgtgttcg
     1141 atgccaacat cagccgggac atgcgccaaa accggcatcc ccaactcggc ggcggcagca
     1201 acgatgctgt caaacgtttc cctgctgaga taccaattga ctttgatgat gtcgtatcct
     1261 tcctcatggg ctttatagac cagcgctctg ccggtggcag gatcggttgg gatattcgtc
     1321 gcggctcccc aatctcccgc gaaccaggga ccgacagcct tgtcgacgtt atcaaacaag
     1381 acctgtacgc ggtccgaggc accgataatg ctctttgccg ccatgatcgt gggtcccagc
     1441 cgctgaccgg ctgcaatttg gtcacgggct tcgagaattt ccatcccggt gccgacatag
     1501 tccgcgttag ggtcgcgtat ggtggtgaca ccattggcaa tcgccagaat caatgaatcc
     1561 tggttatcca gaatatggaa atgagtatct gtcagaccag ggatgagata tttggcattt
     1621 ccatcaatta cttgcgctcc aggcgggacg ctgacctctt ttgccgcgcc gatttcgacg
     1681 atacgatctc cctgcacaat gactgtctgc gattccaaga ctcgttccgt atccatcggg
     1741 atgacgttga cattcacaaa ggcaacggtc tgcctgctct gagccagagt tgtagatgcg
     1801 gggtggacag tcaggagaat caggaagacc atgccagcca cctttaccca ggtattcagg
     1861 tgaaacacaa aggttctctg tattgaaacc tttgtatgct ttatgcctat gcttttcatc
     1921 tcctagtctc cgtataattt ttgatagagt gcgtcttgag aaccgttgta ggttacccca
     1981 gtggcgagcg cataaatgat ttcctgagat tcgctcaatc caccaggata agtcgcggct
     2041 gagatgaagg caaaaacgtc cttgccggct ctaaatacaa gggtcattcc ctcgcgcccc
     2101 tcctttgaga aggttagcat cccggcatcg cgtccatcac ccaatgttac taacgctgtt
     2161 tcacccgcga cattccttgc tggctcatcc ccgactacga acatgggtaa gagtgactgt
     2221 aagaacgcat ccggtgaaga atcggattgg attcccaaat gcgagagttc tttctcttga
     2281 aaaagagcca gagggaggaa tccaacattc agcacaagtt cacccgattc gagagcgttc
     2341 ctgttgcgat aacggtcaag agccgcctcc gtactcgcca tgacgaggtt ggcaccgggg
     2401 atgacttcat gcgcgctcca gccagttggg acatataggc tgaataattt cgtggaagag
     2461 acaaattccg tggacgggaa gctgggttct tttgcctgcc ctttcccgct gcacgccgcc
     2521 aggacaaaga gtgccgccaa taaaataatg agaacaaata tcttgtgcaa aaaccgcttg
     2581 attttcatga actctcctga gaaataaatt gaattgatat tagacctcgg atgctccgtc
     2641 attttgattt tgatcttctt ttcgttcaat atgattcaca gacaccagcg tcaatccgag
     2701 atcacggatg cggctgatca aaccatgcaa tgccgattga tcctccatgt agcccttgat
     2761 cagggtctgc accggttcag cggtttcgtc aacagcaatc tccattcctt cgaaccacgc
     2821 tgcgctttgc ggactgatgc ggcttcgcac tcgaaattcg tagtaagcag gtttgttgaa
     2881 ttcgggtaag ggatccataa gctgtcttct taggcactgg gcttttgcca cttccgacaa
     2941 aagcccagtg aagaggaatt ggtggagagt cgaattcctg ataatgttca gcgcactcct
     3001 aatcggcagg ttgacttaca gctcttgatg cggcgagtgg cttgtccatt gtgccaacag
     3061 gcaccccgtt aatggtaact gcgatcggtt ttctcatgag atatatggcg gtgacaacac
     3121 tcggtatcca ttcgatgaga tattccgaca agggtatttc agccattgtg ataaccccat
     3181 caggtaatcc tgcggtgacc cagaacaaat cgtgaaccgt atggaggatg atcaagggcc
     3241 agatattgtt gacccgcaag cgcacggctc caagcatcac cccatagaga aatgcattga
     3301 gaatctgaac caggttatcc tccaacgaca tcccaccacc cagggactga acgatgtggg
     3361 ccgcgccgaa caaaatcgcc gggatgagga ccgcttttag ctttccatgc ggcagaaatg
     3421 cccttatgaa caagccgcgg aacacagcct cttcgcttat cccggtgaaa agcacggcca
     3481 ggagaaatat gtttactcgc tgaggttcca tcgtgtaagt tccaaagaat accagcggga
     3541 agaaagtaag gatgagcgga accgccagcg cgtacacatt ctgggttgta ttgaccaaac
     3601 cggcatcctt ccaccagccg atctgccaga taaagataag cgatacgatg gcgatcactc
     3661 ccattgcgga tgtcctgatc accaaatcgg gaagccctag cactctacca aggatggcaa
     3721 ccacaaatcc gagaatctgc attacgatga tggtaaagag cagtgagaac caaaaggggt
     3781 gtctttcgat aaagcttttc atgatcttcg ctccttcttt gaggtatgga attttaaaaa
     3841 tactacattc attagcactg aatgtagcca aaaaaggtat ggtttgtcac catacaaagg
     3901 tatggggttg gtatgggatt atcgaaaggt tataaaattc ccaattctct cgcgcgtgtt
     3961 actgcgtgcg cccttcgatg tacccccaac ttgccataga ta
//
LOCUS       DBNGSX010000173         6459 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1329034, whole genome shotgun sequence.
ACCESSION   DBNGSX010000173 DBNGSX010000000
VERSION     DBNGSX010000173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6459)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6459)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6459
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1329034"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /gene="holA"
                     /locus_tag="AC16CB_05510"
     CDS             <1..974
                     /gene="holA"
                     /locus_tag="AC16CB_05510"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR3341958.1"
                     /translation="DDPPVVYVLHGEDEFGIASFLADLSGKLGDATTAAMNTTRMDGN
                     TMTLEGLREAAMALPFLASRRLVVVTNPRARLNSPEIQEKFFHLLDQVPVSTAVILVE
                     GRTLKEDDWLLRWAMGAGSRGYLRSFAQQKEGEMVQFILQQARQAGGQFAPAASWRLA
                     RMVGSDPRLAFQEVHKLLAYVNYKRPVEPEDVETLSVPVFHDKIFALVDALAVQDGHK
                     ASGLLRRFLEEEDPMYVMAMIVRQFRLLLLAREILDQGGMAKDVQSRLKLKRFEASKI
                     TEQARRFSLAALEGVYRSLRDIDEAIKSGRIEDEVALDSMVAGFTTR"
     gene            complement(961..1227)
                     /locus_tag="AC16CB_05515"
     CDS             complement(961..1227)
                     /locus_tag="AC16CB_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341959.1"
                     /translation="MNIGMLWFDNDPKTELNAKIERATSYYRNKYGKTPNVCFVHPSM
                     IGASPTSNGNETLKSGNVEVRISPSIRPNHFWIGVNGTLQANES"
     gene            complement(1224..2036)
                     /locus_tag="AC16CB_05520"
     CDS             complement(1224..2036)
                     /locus_tag="AC16CB_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341960.1"
                     /translation="MLSTQERLEIFLEVLERVAPEMGARAMYNLNTPVRDINQSLSGL
                     LERLSPLPPYSALIGMCEDGLPFLLDLSDTSPGSILIIADEQSGQTRLLQTILESACI
                     LNSPAKVVCSVISPNPGQFESLEAMDNCRAILSTYDRAASEMVVELAELADQRIYGRN
                     QGSVELLAIDDLAAFVQNNDHEINCYLKWLVAHGSQGAVWPISSIKMNQLWRIRDGMY
                     EAFGTHFIGRVSTSQLPAGLGAAPPHFEIPGVFSTLMDGEWIHFWVPERNSL"
     gene            2332..2628
                     /locus_tag="AC16CB_05525"
     CDS             2332..2628
                     /locus_tag="AC16CB_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HIR3341961.1"
                     /translation="MATYVSLISYTQQGMQKIKESPARLDEAKKVFKSMGAELKAWYL
                     LVGEYDALVISEGPDGETVAKLLLMIGALGNVRTHTMLAFTENEYRKIVAGLPG"
     gene            complement(2762..4006)
                     /locus_tag="AC16CB_05530"
     CDS             complement(2762..4006)
                     /locus_tag="AC16CB_05530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HIR3341962.1"
                     /translation="MSSSSILEVELNSLTYGGDALGRLPDGRIVFVPLGLPGEKVAIR
                     LVEDKRRHARGEVVEILRPSPERVEARCRHYGTCGGCHYQHLPYVAQLAAKQAIVAEQ
                     LERIAGISNPPVGQTIASPLAWGYRNFMQFHQTSQGALGLQKPRSNEVFPLQECHLPE
                     EALNTTWPMLEFEPIPGLDRIGLRAGAGDELQLILESKLPETPELSIEDLPLSVVHLS
                     PWGSLLLAGSQFIEIEVLGRPFRVSAGSFFQVNRLVAEAMVEHLLSNLSLETSTTLLE
                     LYCGVGLFSAFLAPRVARFVGVEASPQACSDFEYNLDEYDNVELYEASAEHVMESLDM
                     HADIVLVDPPRAGLRRDVMDGLLALRPQQIAYISCDPATLARDARALIDGGYDLVNIT
                     PFDVFPQTYHIESISWWIKSEI"
     gene            complement(3978..5054)
                     /locus_tag="AC16CB_05535"
     CDS             complement(3978..5054)
                     /locus_tag="AC16CB_05535"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HIR3341963.1"
                     /translation="MSRMFVPGPVDVDPQVLYAQTRPMLPHRSKEFEAIFQRASEKAR
                     QLFYTQQRVFLMTSSGSGLQEAAVRNFAKESVLSCVNGAFARRWYETARANGKQADLL
                     EVEWNRPISGDLVASALKSKHYEMLTVVHNETSTGLQNPVQEIAAAARSVSPDTLICV
                     DAVSSLSGAKIEMDAWGIDMLLTSSQKCLALPPGLALGAVSDRALAYAEGVPNRGWYF
                     DLVLMEKHRLKDSTPATPALSLIFALDLQIDRIMAEGLENRFARHSAMAERVQNWAAQ
                     RGLGLHAPEGYRSQTVSAVLNTLEWDIPALNKFLLEREMRIANGYGPLKGKTFRIAHM
                     GETQLDDIQKLLAALDEFIKHPRS"
     gene            5159..>6459
                     /locus_tag="AC16CB_05540"
     CDS             5159..>6459
                     /locus_tag="AC16CB_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341964.1"
                     /translation="MRRTLIPFVMVLLLACRAASSLPSSLAPATLTPTLPPTVTPAPT
                     YTPSPNLPRATYDVRFHPDGGLYVGDQVSFEVIAPPETNLRGRSAVVEVQGVEDAVFG
                     PVEFERYGIAGRVQSTMTWAWDTSGLLPGDYAVTFSIEPNGPSWTETVALLPEDALPA
                     VEQRARWARAESNCCLLYYITGSEAERDLAELLQMADREAATVEERLDTNLSEVVGLV
                     ILPRILGHGGFANQDLSVSYLDRRYTGGEPATILRHELVHFLDNQLGGELRPTMLVEG
                     LAVYLSGGHYKPEPLLPRAAALLELGWYLPLDELADDFYPSQHEIGYMEAGALVEYMV
                     ERWGWEAFNDFYRDIRSGPDQVEAMDKALVRHFGITFSRLEDDFIGALGEQAVSEAIE
                     DDVRLSVNFYDTARRYQQALDPSAYYLTAWLPNGKEMRERG"
BASE COUNT         1369 a         1740 c         1937 g         1413 t
ORIGIN      
        1 ctgacgaccc ccctgtagtc tacgtcctgc acggcgagga cgagttcggc attgccagct
       61 tcttggccga cctgtctggc aagctggggg atgctacgac cgcggcgatg aacactaccc
      121 gcatggatgg caatacaatg accttggaag ggttgcgcga agcagccatg gccctgccat
      181 ttttagcgtc ccggcggcta gtggtggtga cgaacccaag ggcgcggctt aattctcccg
      241 aaattcagga gaaattcttc cacctgctgg accaggtgcc tgtctctact gcagtgattt
      301 tggtggaggg acgcaccttg aaggaggatg attggttgtt gcgctgggcg atgggagcgg
      361 gttccagagg ctacctgcgc tccttcgctc aacagaaaga gggggagatg gttcagttta
      421 tactgcagca agccaggcaa gccggagggc agttcgctcc tgcggcgtcc tggaggctgg
      481 ctcgcatggt tgggtccgat ccccgcctgg ctttccaaga agtgcacaaa ctcctggctt
      541 atgtaaacta taaacgcccg gtagaacccg aagacgtgga gacgctcagc gtccctgtct
      601 ttcacgataa gatttttgcc ctggtggatg ccctggctgt gcaggatggt cataaagcga
      661 gcgggttatt gcgccgtttt ctcgaagaag aagacccaat gtatgtaatg gcgatgatag
      721 tgcgccagtt ccgcctgctg ctgctggctc gtgagatact cgatcaggga ggaatggcaa
      781 aggacgttca atcccggttg aagttgaaac gcttcgaagc cagcaagatc acagagcagg
      841 cacggcgctt ctccttagcg gcgctggagg gagtttatcg cagcttgcgg gatatcgacg
      901 aggcgatcaa gtctggtcgg attgaggacg aagtagcttt ggacagcatg gtggcgggct
      961 tcacgactcg ttagcctgaa gtgtcccatt aacgccaatc caaaaatggt tggggcggat
     1021 agacggggag atgcgcactt cgacatttcc gcttttcaac gtctcatttc cattagacgt
     1081 aggtgatgcg ccgatcatgc tgggatgcac aaagcacacg tttggcgtct taccgtactt
     1141 attacggtag tagctggtag cgcgctcgat cttggcattc aactcggttt tggggtcgtt
     1201 atcaaaccag agcataccga tattcatagg ctattccttt ccggaaccca gaaatggatc
     1261 cattcgccgt ccatcagggt tgagaatacc cctggtattt caaaatgtgg gggtgcagcc
     1321 cccaatccag cgggcaattg agacgtggaa acccttccta tgaaatgggt tccaaatgct
     1381 tcatacatcc catcacgaat ccgccatagt tgattcatct tgatcgagct gatgggccaa
     1441 accgccccct gggatccgtg tgcgacaagc cacttgagat aacaattgat ttcgtgatcg
     1501 ttgttctgaa cgaatgctgc caggtcgtcg atagccagga gctcaacact gccttgattc
     1561 ctgccataga tgcgctggtc agccagctca gccagctcga caaccatctc gctggcagcc
     1621 cggtcgtagg tcgacaggat tgcccggcag ttatccatcg cttctaagga ttcgaactga
     1681 cctggatttg ggctgatcac cgagcagaca acctttgctg gtgaatttaa gatgcaggca
     1741 gattctagaa tggtctgcag caggcgtgtc tgtccgcttt gctcatcggc gatgatgagg
     1801 atagagcctg gtgaggtgtc cgataaatcg agcaggaagg gtagcccgtc ttcgcacatg
     1861 ccgatcaaag cagagtaggg cggcagcggg cttagacgtt ctagcaagcc gctcagggat
     1921 tgattaatat ctctcaccgg tgtgttaagg ttgtacatgg cgcgtgctcc catttccggg
     1981 gctacccgtt cgaggacctc aagaaagatt tccagacgct cttgggtgga taacatcgca
     2041 attgtattat aacagaacgt atgttctatg tcaagagatg cgcgcagaaa tttccccctt
     2101 ctgtgggggg aagccgctct catgcgattg cctgcctgag ctttgacagg cagtatattc
     2161 ggtcttataa tttgagtgag ccacacatct atttattcca gatcggccac atcactacaa
     2221 ttcaaaacgg atgccactgg cggtatgtta cagcttgaac ccaattgctc agttgggttt
     2281 gtggatatta ggcaaattga ggcaggcgca aatagcaatg gaggaaggaa aatggctact
     2341 tatgtgtcct tgatcagcta tacccaacaa ggtatgcaaa aaatcaaaga gagtcctgcg
     2401 cggctggatg aagccaaaaa ggttttcaaa tctatgggtg cagagttgaa agcgtggtat
     2461 ttattggttg gtgagtatga cgcgcttgtc atcagtgaag gtccggatgg tgaaacagtc
     2521 gctaaattgc tcttgatgat cggcgcgtta ggaaatgtgc gcactcatac gatgctggct
     2581 ttcacagaaa acgagtacag gaagatcgtc gctggtttac cgggataata taaatcgagt
     2641 aagtcaatca atctgagatc agctgcgctg cctcatagag caactcctgg cggaaggtta
     2701 ttctgccagg agttttttgt aggaaaagcg agagtatgca gcgagcagcc tcaccgacta
     2761 ttcaaatttc agatttgatc caccagctga tgctttcgat gtggtaggtc tgggggaaaa
     2821 cgtcgaaagg ggtgatgttg actaaatcgt atcccccgtc gatcaacgcc cgagcgtcac
     2881 gcgccagggt agccgggtcg caggaaatat aggcgatctg ctgaggccgc aaggcgagca
     2941 atccatccat cacatctcgt ctcaagccag cgcgtggggg atcgaccagc acgatgtcgg
     3001 catgcatgtc caggctttcc atgacgtgtt ctgcggaagc ctcgtacaac tccacgttgt
     3061 cgtactcgtc caggttgtat tcaaagtcag agcaggcctg aggggaggcc tcaaccccca
     3121 cgaagcgcgc cacgcggggt gctagaaagg cgctgaacag tcccacgccg caatacagct
     3181 ccagcagcgt tgtgctggtt tccagggata agttcgacaa aagatgctca accatggcct
     3241 cagccaccaa gcggttgacc tggaagaatg acccggcgga gacgcgaaaa gggcggccca
     3301 agacttcgat ctcgatgaac tggctgcccg ctaataaaag cgagccccac gggctgaggt
     3361 gcacaaccga gaggggcaag tcttcaatgc tgagctctgg agtctctggc agtttgcttt
     3421 ccaggatgag ctgcagctcg tccccggcac ccgcccgcag cccgatgcga tccagaccgg
     3481 gaatcggctc gaactccagc atcggccagg tggtgttgag cgcctcttcg ggcaggtggc
     3541 actcctggag cgggaagacc tcgttggaac gcggtttttg gagaccaagt gcaccttggg
     3601 aggtctggtg gaactgcata aagttgcgat agccccacgc cagaggcgag gctatggtct
     3661 gccccacagg cgggtttgag atgccagcga tgcgctccag ctgttcggcc acgatcgcct
     3721 gcttggcagc cagctgcgcc acatagggta ggtgctggta atggcagccc ccgcaggtgc
     3781 cataatgccg gcagcgggct tcgacgcgct ccggcgatgg acgcaggatt tccaccacct
     3841 ctcccctggc atggcggcgt ttgtcttcca ccaggcggat agcgactttc tcgccgggca
     3901 ggcccaaagg gacaaacact atacgtccat ccggcagcct gcccagggca tcgccgccgt
     3961 aggtcaggga attcagctca acttcgagga tgcttgatga actcatccag cgccgccagc
     4021 agtttctgga tatcgtccag ctgggtttcg cccatgtggg caatgcggaa ggttttgccc
     4081 ttcagcggcc cgtagccatt ggcgatgcgc atctcgcgct ccagcaggaa cttgttgagg
     4141 gccgggatat cccactccag ggtattgagc actgcggaga cagtctgcga gcggtaaccc
     4201 tcgggggcgt gcagccccag cccgcgctgc gcagcccagt tctgcacgcg ttcagccatg
     4261 gcgctgtgac gggcaaagcg attctccaac ccctcagcca tgatgcggtc aatttgcagg
     4321 tctagggcga agatcaggga gagggctggt gtggcggggg tggagtcttt caggcggtgc
     4381 ttctccatca ataccaggtc gaaataccag ccgcggttcg gcacgccctc ggcgtaagcc
     4441 agagcccggt cagagactgc ccccaatgcc aagccggggg gtagcgccag gcacttctgt
     4501 gaagaggtga gcagcatatc gatgccccag gcgtccatct cgatctttgc cccgctcagc
     4561 gaggagacgg catccacgca gatcagcgtg tctgggctga cgctgcgggc tgccgcggcg
     4621 atttcctgga cgggattttg caagccagtg gaggtctcgt tgtgcaccac ggtgagcatt
     4681 tcgtaatgct tgctttttag agctgaagcg accaaatcgc cgctgatggg gcggttccat
     4741 tccacttcca gcaggtcagc ctgcttgccg ttggcgcggg ctgtttcata ccagcgcctg
     4801 gcaaaggcgc cgttgacgca ggagagcacg ctctctttgg cgaaattgcg cacagcggct
     4861 tcctgcagcc cgctgccgga gctggtcatg agaaagaccc gctgctgggt gtagaacaac
     4921 tggcgcgcct tttccgaagc acgctggaag atagcctcga actccttgct gcggtggggc
     4981 agcatgggtc ttgtctgggc atataaaacc tgcgggtcga catcgaccgg gccggggaca
     5041 aacatgcggg acataacgcc tccgagaaac gtgcctatag attcgagtgg ggatatatat
     5101 ccagacccca ttgtactatt atcttctctc tattctcgaa tcccttataa ttgcccccat
     5161 gcgtcgcacc ctgataccct ttgttatggt cttgctgctg gcttgccggg ctgcctcttc
     5221 cttgccatcc agcctggctc cagctaccct aacgccgacc ctgccgccaa ccgtcacgcc
     5281 tgcgcccact tacacgcctt cgcccaactt gccaagagca acctatgatg tgcgctttca
     5341 ccccgatgga ggcctgtatg tcggcgacca ggtgagcttt gaggtgattg cacccccgga
     5401 gaccaacctg cggggtcgca gtgctgtggt agaagtgcag ggggtagaag atgctgtctt
     5461 tggaccggtg gaattcgagc gctacggcat tgcggggcgg gtgcagtcca ccatgacctg
     5521 ggcttgggat acgtccggtt tgctgccggg cgattatgct gtgacattct cgatagaacc
     5581 caatgggcca agctggactg agacggtggc gctgctcccc gaagatgcct tgcccgcggt
     5641 tgaacagcgg gcgcgctggg cgcgcgccga gagcaattgc tgcctgctct actacatcac
     5701 cggctccgag gctgagcgcg atctggctga attgctgcag atggcggatc gagaagctgc
     5761 tacggtggag gagcgcctgg ataccaattt atctgaggtg gtggggctgg tcatcctgcc
     5821 gcgcatcttg ggacatgggg gctttgccaa ccaggactta tccgtgtcct atcttgaccg
     5881 gcgttacacc ggtggcgaac cggcaaccat cctgaggcat gagctggtcc attttttgga
     5941 taaccagctt ggaggcgagt tgcgcccaac gatgctggta gaggggctgg cagtgtacct
     6001 gagcggtggg cactacaaac cagagccgct cctgccgcgc gctgccgccc tcttggaact
     6061 gggctggtac ctccccctgg acgagctggc agatgatttc tatccctccc agcacgagat
     6121 cggctatatg gaggccggcg ccctggtgga atatatggtg gagcgttggg ggtgggaggc
     6181 tttcaacgat ttttaccgag atatccgctc tggccccgac caggtggagg caatggacaa
     6241 agcgctggtc aggcattttg ggatcacctt ctcccggctg gaagacgatt tcataggtgc
     6301 cctgggggag caggcggtca gcgaggcgat tgaagacgat gtgcgcctga gcgtgaactt
     6361 ctacgatacc gcccggcgct accagcaggc gcttgaccca tcggcctatt acctgaccgc
     6421 ctggctgccc aatgggaaag agatgcgaga gcgggggat
//
LOCUS       DBNGSX010000174         2409 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_428700, whole genome shotgun sequence.
ACCESSION   DBNGSX010000174 DBNGSX010000000
VERSION     DBNGSX010000174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_428700"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="AC16CB_05545"
     CDS             <1..525
                     /locus_tag="AC16CB_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR3341965.1"
                     /translation="VEREESGAEVLPNKLSNVILYNEAESSNRRSPRDFTLSMGMGMR
                     WMINGRTFEMEGVARDEVVKLGDLETWQFTNQVGGGMGMMGGMALPHPMHVHGLQFQI
                     VERDIDASGRAAWETVSDGFVDEGWHDTVLVMPGERVKILLKFEDFEGLYLYHCHNLE
                     HEDMGMMRNYRVDS"
     gene            complement(564..1496)
                     /locus_tag="AC16CB_05550"
     CDS             complement(564..1496)
                     /locus_tag="AC16CB_05550"
                     /inference="COORDINATES: protein motif:HMM:NF025063.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HIR3341966.1"
                     /translation="MSGSSGHLVIERLKESGHEPDYPGKVFANPELSYTEAMQAFVDA
                     SHPLSGPRKRVAAAEHRSQRAEIRELRREEAELCAERRRLRQHRKLEDAAWQHQRRAQ
                     QSVEEPAQPKLRARWGSRKRQFELQQALREQRHLQLALRQQEDEQWRLARQSLRERMA
                     RLPIVTAWIAVLVVTDNCSRQCLGLPLFVAGSHVTAEMIVAALRVLLPANLQFLISDR
                     GIHFTAKVFQQLAIEASFIHVVIARHRPQSNGIAERFVRTIKEWLADKVWSSDQELLA
                     LLCRFLPEYNDRPHQGLPVPGLSPNEFAKRLWLM"
     gene            complement(1586..2005)
                     /locus_tag="AC16CB_05555"
     CDS             complement(1586..2005)
                     /locus_tag="AC16CB_05555"
                     /inference="COORDINATES: protein motif:HMM:NF024954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3341967.1"
                     /translation="MKAKRVYFPATTPQQRKLLFETWEQSKNVTAACRKAHVGRSTFY
                     YWKPRFEEKGYAGLETYEKLGVPPGTGRVTDEIKNKVLELHGAHADWGKRRLADEMTK
                     SNNWVPLISPNSVRRILREAGMWEPDENKVKKASSRQ"
     gene            complement(2042..>2409)
                     /locus_tag="AC16CB_05560"
     CDS             complement(2042..>2409)
                     /locus_tag="AC16CB_05560"
                     /inference="COORDINATES: protein motif:HMM:NF024954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3341968.1"
                     /translation="EEPGLKKKLAGFGGGNVERLIRYKPEEKLEIIHLVEHSNLPVKQ
                     TLEELQVSRSTFYDWYRRYQELGPEGLKNRHSKGDQFWNRIPDPVREQIVDLALKYPE
                     EAPRQLTYRFIDENAYFVY"
BASE COUNT          565 a          624 c          603 g          617 t
ORIGIN      
        1 gtggaacggg aagagagtgg agcagaagta ttgcccaaca aactttcaaa tgtcattctc
       61 tataacgagg cagaatcttc caaccgtcgt tcacctcgcg acttcaccct atcaatgggc
      121 atgggaatgc gctggatgat caacggtcgc acgtttgaga tggagggtgt tgccagggat
      181 gaagttgtaa agctagggga tttggaaaca tggcaattca ccaaccaggt gggcggcgga
      241 atgggaatga tgggtggtat ggcattgcct catccaatgc acgttcatgg gctgcagttt
      301 cagattgtgg aacgggatat tgatgcatct ggtcgtgcag cttgggaaac ggttagtgac
      361 gggtttgttg atgaaggctg gcatgatacc gtgctagtca tgccgggaga acgcgtcaag
      421 attttgttaa aattcgagga ctttgaagga ttgtatctgt atcactgcca taaccttgaa
      481 cacgaggata tgggcatgat gcgtaattat cgagtggatt cataagtaac tgtattggtc
      541 ataaaaaggt tgaacaccac cggttacata agccagagcc gcttggcgaa ttcattggga
      601 gacaagcccg gaactggcag tccctgatgc ggacgatcat tgtactcggg caaaaaccgg
      661 catagcaatg ccagtaattc ctggtcggac gaccagactt tgtcagccag ccattctttg
      721 atcgttcgga caaagcgctc ggcaatccca ttcgactgcg ggcgatggcg agcgatgacc
      781 acatggataa agctggcctc gatcgccaac tgctgaaata ctttggcagt aaagtgaatg
      841 ccacgatccg aaatcaagaa ctgcaagttt gcaggcagca aaacgcgtaa ggcagcgacg
      901 atcatttcgg cggtgacatg cgagccagcc acaaaaagtg gcaagcccaa acattgccga
      961 gagcaattgt cagtcacaac cagtacagca atccaggcgg tgacaattgg cagcctagcc
     1021 atcctttctc gcagactttg tcttgctaag cgccactgtt catcttcctg ttggcgcaag
     1081 gccagttgca gatgacgttg ttctcgcagc gcctgttgca gttcgaattg tcttttgcga
     1141 gaaccccatc tggcacgcaa tttgggctgg gcgggttctt ccacagattg ttgtgcgcgg
     1201 cgttggtgct gccaggccgc atcttccaac tttcggtgtt gtcgtaaacg acgccgttcg
     1261 gcacataatt cggcttcttc ccggcgaagt tcccggattt cagcccgctg cgatcggtgt
     1321 tcagccgcag ccaccctctt gcgcggtcca gaaagcgggt gagaagcatc tacgaaggct
     1381 tgcatcgctt ccgtgtaact cagctcagga ttggcgaaca cttttcccgg atagtccggt
     1441 tcatgcccgc tctctttgag ccgctcgata acaagatgac cggatgagcc gctcacagct
     1501 ggcaacttga catctgtctg atggcaggct ggcacaaaaa gcaggtcgac gttgagcgcc
     1561 tggccaggtt gatcagccgt acgatttact ggcgcgaact cgcttttttt accttgttct
     1621 catctggctc ccacatcccc gcctcacgca ggatccgccg aacgctgttc gggctgatca
     1681 agggcaccca gttattgctt ttagtcattt catccgccaa ccgtcttttg ccccaatcag
     1741 cgtgggcacc atgcaactcc aaaaccttgt ttttgatctc atccgtaact cgacctgtcc
     1801 ctggcggaac gcctaatttc tcgtaggttt ccagacccgc atatcctttc tcttcgaatc
     1861 gcggcttcca gtagtaaaaa gtgctccgcc ctacatgcgc tttgcggcag gccgcagtaa
     1921 cattcttgct ttgctcccag gtttcaaaga gcagtttccg ctgctgcggt gtggttgctg
     1981 gaaagtaaac acgttttgct ttcatcctta tataatactc tagtgtccaa gctttctatg
     2041 actaatacac aaaataggca ttttcatcga taaaacgata agtcagctgc ctgggtgctt
     2101 cttcagggta tttcaaagcc agatccacga tctgttcacg cactggatca ggaattcgat
     2161 tccaaaattg atcccccttt gagtgtctgt ttttcagccc ctcaggaccc agttcctgat
     2221 agcgccgata ccaatcataa aaggtgctgc gtgatacctg cagctcttct aaggtttgct
     2281 tgacaggcaa attagaatgc tccaccagat gaatgatctc caacttctct tctggcttat
     2341 atcgaatcaa tcgttccacg tttcctcccc caaaccccgc aagctttttt ttaagacccg
     2401 gttcttcaa
//
LOCUS       DBNGSX010000175         2401 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1605803, whole genome shotgun sequence.
ACCESSION   DBNGSX010000175 DBNGSX010000000
VERSION     DBNGSX010000175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1605803"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="AC16CB_05565"
     CDS             <1..281
                     /locus_tag="AC16CB_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341969.1"
                     /translation="MPWQAPGSLSEDEYWQLTAFLVQANGFELGTEPLDRERAAKVFL
                     GLKPTPTTTPALFLESSGFGFWVVSGGVLALIALLVILKLRAGLGRNN"
     gene            389..970
                     /locus_tag="AC16CB_05570"
     CDS             389..970
                     /locus_tag="AC16CB_05570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3341970.1"
                     /translation="MLEVTDKALVIQLQEGNLEALGALYDRHRRLVYRTALAITGDAD
                     SAADLLQDVFLRLNRFSRNIDPLRPLEPWLYRMTTNLSYDWVKRRKRWPRPLEDLADW
                     LIGSVKNSPYEVVEKNDEWSRVQRAVAALPLPQRVVVVLYYLNDLALQEIAEILDVPI
                     GTVKSRLYYGRLGLKRSLGLEQDLVPDLQFEQT"
     gene            981..1229
                     /locus_tag="AC16CB_05575"
     CDS             981..1229
                     /locus_tag="AC16CB_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341971.1"
                     /translation="MKNLFDATIRKAMKEWADQHEPPKQGKARLLMLAATSASKHIHT
                     PTFRKRDFYSLSQTSSNLASSMVAEKFHSPWLWMFNVA"
     gene            1326..>2401
                     /locus_tag="AC16CB_05580"
     CDS             1326..>2401
                     /locus_tag="AC16CB_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3341972.1"
                     /translation="MIKPSQLFSPFPWIVLVFLLSSCTLPTSTPIPETTPLDPTAQSP
                     TLQALIPPTTPPPRALTVCLGQEPTSLFIYADGSSAARSVRQAIFDGPAEIVNYELQP
                     VILERLPNLANADVLLEPTQVVSGTLIVASDGSLANLEEGVVFLPAGCNRAECAQSYA
                     GEAAVSMEQLVVRFRLRPGLLWADGVALTAADSVFSYEVARALYPRARPNLIDFTHSY
                     LALDELTVEWRGLPGYKDPLYQTNFFVPLPQHLWGSIPPGELLSAEISSRMPLGWGPY
                     MIEEWTSGDHITLIKNPNYYRASEGLPYFERLVYRFIGQGETALSALLAGECDFVDES
                     GLDGIPHQRLFQLQDEGRIKVIAEP"
BASE COUNT          517 a          626 c          654 g          604 t
ORIGIN      
        1 gcatgccctg gcaggcgccg ggcagcttga gtgaagatga atattggcaa ctcaccgctt
       61 tcttagtgca ggcgaacgga tttgagcttg ggacagagcc cctggatagg gaacgagcgg
      121 ccaaggtgtt cctgggcctc aaacctaccc cgaccaccac gccagccctg tttctcgaaa
      181 gctccgggtt tggcttttgg gttgtctctg gtggggtgct ggctctcatc gctttgctgg
      241 tgattctgaa gctgcgcgct ggtttgggta gaaataattg aagttttgct gctgcttttt
      301 ggtataataa tcaaaggttt gggtttcttt tattggtttc gggtggtagc cgtctaaaag
      361 tgattgtata agcatgggtg aggtgataat gctagaagtc acagataagg ctcttgtcat
      421 tcagctacaa gaaggtaatc ttgaagcact gggcgcgctc tatgatcgcc accgccgctt
      481 ggtttatcgg actgcgctgg cgattacggg ggatgcggat tcagccgctg atcttttgca
      541 agatgtattt ttgcgcttga atcgtttttc gcgtaacatc gatccgctgc gcccgctgga
      601 accctggtta tatcgcatga cgactaatct gtcgtatgac tgggtgaaac gccgcaaacg
      661 ttggccacgc cccttggaag atctagcaga ttggctaatt ggctcagtga aaaactctcc
      721 ttacgaggtg gtagagaaga acgacgaatg gtcccgggtg caacgggcag ttgccgcact
      781 gcccctgccc cagagggtag tagtggtttt atattacctc aatgatcttg cccttcaaga
      841 gatcgctgag atcctggatg tgcccatcgg cacggtgaaa tcccgcctgt attatggtcg
      901 ccttggattg aaaaggagcc tggggctgga acaggatctg gtaccagact tacagtttga
      961 gcaaacatag gtgaagtgag atgaagaatt tatttgacgc gacgattagg aaagcaatga
     1021 aggagtgggc ggatcaacat gagcccccga aacaaggaaa ggcgcgctta ttaatgcttg
     1081 cggcgacaag cgcttcaaaa catatccata cccccacatt tcgcaaacgc gatttttact
     1141 cgctatcgca aacgagttcg aatctagctt ccagcatggt cgctgaaaag tttcatagcc
     1201 cctggctatg gatgttcaat gtggcctgat tttgcagcct cttggctggg tcagcagttt
     1261 aactgctgac ccagttgtgt gcaagccccc attttatcga aactcattcc ctataaggtt
     1321 gccctttgat taagccatcg cagctattct cgccattccc ctggatagta ttggtatttc
     1381 tcttaagttc ttgcacgctt cctacatcta cccctatccc cgaaacaacc ccactagatc
     1441 ctactgctca atcacctacc ctgcaggctc ttatcccacc gacaactcca cccccgcgcg
     1501 ccctgacggt ttgcctgggg caagagccca catcgttgtt tatatatgca gatggctctt
     1561 cagccgcccg tagcgtgcgc caggcaatct ttgacggacc agcggagatc gttaattatg
     1621 agctccagcc ggtgattttg gagcgcttac ccaacctggc aaacgcagat gtccttcttg
     1681 agccaaccca ggtggtcagc ggcacgctga tcgtcgccag cgatggtagt ctggctaacc
     1741 tggaggaggg cgtggtgttc ttgccggcgg gctgcaatcg ggctgagtgc gcccaatcct
     1801 atgccgggga ggctgccgtg agcatggaac aactggtggt gcgcttccgg ctgcgacctg
     1861 gtctgctgtg ggccgatggg gtcgctttaa ctgccgctga ttcggtcttc tcgtatgaag
     1921 tagcgcgtgc cctgtacccg cgcgcccgcc ccaacctgat cgactttacc cattcctatc
     1981 tagcactgga cgagctgaca gtggaatggc gcgggcttcc cggttacaaa gatcccctgt
     2041 accagaccaa cttctttgtt ccgctaccac agcacctgtg gggtagtatt ccccctggtg
     2101 aattgttgag tgccgaaatc tccagccgta tgccacttgg ctggggaccg tatatgatcg
     2161 aggaatggac atctggcgat catatcacgc tgattaagaa tcccaactac taccgcgcct
     2221 ccgaggggct gccgtacttc gagcgcctgg tgtatcgatt tattggtcaa ggagaaacag
     2281 cactttcagc gctgctggca ggcgaatgcg attttgtgga tgaaagcggg ctagacggca
     2341 ttccgcacca gcgcctgttc cagctacaag atgaagggcg tatcaaagtg attgcagagc
     2401 c
//
LOCUS       DBNGSX010000176         7945 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1744770, whole genome shotgun sequence.
ACCESSION   DBNGSX010000176 DBNGSX010000000
VERSION     DBNGSX010000176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7945)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7945)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7945
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1744770"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..590
                     /locus_tag="AC16CB_05585"
     CDS             273..590
                     /locus_tag="AC16CB_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HIR3341973.1"
                     /translation="MISRVWHGWTTHQNATAYETLLKSEIFTGIQSRQIRGYKGIHLF
                     RRDLIDEVEFVTVMWFDTLEAVRDFAGEDYETAVVPPKARALLSRFDARSQHYEVKAE
                     MRV"
     gene            643..834
                     /locus_tag="AC16CB_05590"
     CDS             643..834
                     /locus_tag="AC16CB_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341974.1"
                     /translation="MSDGNQRTNTLIGLATIGSVVTGVISLLGALITLLSGEFLPAAV
                     FLIAAALAFGLFAIALIRK"
     gene            complement(900..2072)
                     /gene="galK"
                     /locus_tag="AC16CB_05595"
     CDS             complement(900..2072)
                     /gene="galK"
                     /locus_tag="AC16CB_05595"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HIR3341975.1"
                     /translation="MELVIPFQQVFSRRPTLQVRAPGRVNLLGEHVDYNDGMVLPVAL
                     DRAVYLVAAPTEDQIIHLRALDLSKAVSFRADQVEAKVDLEGEPLPGWARYPAGVAWA
                     LQEAGLEVVGAQVAYSSTVPIGAGLSSSAAVEVAFAGLWRAMGGWSVGNLDLARLCQR
                     AENHYVGVACGLMDQFASACGVEGHALYFDTRSLEWQSLPLPAGTVIIVADSGVRRSL
                     TSSAYNQRRQACEQAVELLRQYKPDMRALRDIAPPEFAAYSALLPPEIARRAEHVVRE
                     IARVESAVAALQRQDAQIFGALMYAGHASLRDFYEVSIPELDTLVDITRDLPGCYGAR
                     LTGAGFGGCTVNLVEESQAEAFIQSLSEAYQGRTGRQAQVYLCRASSGVIVKPVLT"
     gene            complement(2139..2312)
                     /locus_tag="AC16CB_05600"
     CDS             complement(2139..2312)
                     /locus_tag="AC16CB_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341976.1"
                     /translation="MKNSKQNKTYEIHSFAFGLMVLSSAGLYFAAGAGSEIWIWGLIG
                     LFVSGNVLVMLVK"
     gene            complement(2318..3847)
                     /locus_tag="AC16CB_05605"
     CDS             complement(2318..3847)
                     /locus_tag="AC16CB_05605"
                     /inference="COORDINATES: protein motif:HMM:NF014683.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HIR3341977.1"
                     /translation="MTFILALTPIVLILFLMVGLRWGAAQAGGAGYLVALLIAILFFG
                     AGPELIAYAHAKALLLAIDVLLVIWAAFLVYRVADEAGAIRTIGQALPQLTNDQGMQA
                     LVIGWIFASFLQGVGGFGVPVAVIAPILVGLGFTPLASVVIPSIGHGWAVTFGSLGSS
                     FLALMAATGLPSTALAPPSALFLGISGLGVGWMVAHATGNWKAVRRLFLPVLILGIVM
                     GIVQYLVAVSGLWNIAAFAGSIAGLAMAFPIARRYRGDQKDNGVLDGRALLVALSGYA
                     ILVLVILGVQLTPGVKSFLGQVVVQVQFPELHTALGFITPAGAGRKIAVFGHTGTLLM
                     YSAILAYVVYRLNGLYKPGSVRRILDGTLRRVMSSSVSIASMVAMAVIMENAGMTDRL
                     ARGLAEGVGVLFPAVAPWIGALGAFMTGSNTNSNVVFGALQLRTAELLGYTAAIILAG
                     QTSGAALASVMAPTKVVVGASTAGMAGREGDVMRKLVIYAVLLVLLISILTSIGVILS
                     I"
     gene            complement(3894..4664)
                     /locus_tag="AC16CB_05610"
     CDS             complement(3894..4664)
                     /locus_tag="AC16CB_05610"
                     /EC_number="2.8.3.-"
                     /EC_number="4.1.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004595063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase subunit beta"
                     /protein_id="HIR3341978.1"
                     /translation="MIEYSSSELLTINAARLLRDGDVVFVGVGLPNLACNLARHTHAP
                     SLVMIYEAGVIGAQPARLPLSIGDPTLVSGALSVCSMYDIFAFYLQRGNIDVGFLGGA
                     QIDRFGNINATVIGDYQHPKVRLPGSGGSMEISAWANRCYILTPHQKRRFPEKVDFRT
                     SAGFLGGRAEREAANLRGGGPQAVVTDLGIMEPDENGELTLTALHPGCTVEQAKANTG
                     WDLKVAAQLRLTDPPSEEELRILRLELDPDGIYLKSSA"
     gene            complement(4661..5098)
                     /locus_tag="AC16CB_05615"
     CDS             complement(4661..5098)
                     /locus_tag="AC16CB_05615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR3341979.1"
                     /translation="MDYRDLKIYQKPREVVQLLNKIMNSWPKTMQSQEIKRQLFRSAT
                     SIGANIAEGHGRHVGQEYIHYLTIAQGSANEVDHWLNTALDCGIGSAKELNNLISLNN
                     ETRKMLNASINTLKKRRDNKIMHETPAPYAPIPYSPENSEEKL"
     gene            complement(5131..6033)
                     /locus_tag="AC16CB_05620"
     CDS             complement(5131..6033)
                     /locus_tag="AC16CB_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HIR3341980.1"
                     /translation="MSKLLSLSEAVARYVQDGDLVYAAGFTHLIPFAAGHEIIRQEKR
                     DLVLARATPDLIYDQVVAAGCARKIIFSYMGNPGVGSLRICRAELEAGNLDWEEYSHF
                     SMISRIQAGATGLPFMPMKPTAAVDLERSNPNYRTVVDPYSGQNVVVVPALNPDVAIV
                     HVQRADAQGNAHIWGIIGEQKEAAFAADRVILTAEEIVDEEIIRSDPNRTLIPGFIVD
                     AVCHVPYCAHPSYTQGYYDRDNAFYLDWDRISQSKEAVQSYLDEWVYGVSDRAEYWEK
                     LGKEVHQRLQVGSRPSATVDYGKY"
     gene            complement(6039..6278)
                     /locus_tag="AC16CB_05625"
     CDS             complement(6039..6278)
                     /locus_tag="AC16CB_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341981.1"
                     /translation="MFKWQVGQFLVLLGLIGLVVFFMTDQVREPIYSFFCYGLVVLFF
                     GGYLMWIGRGPAPPPSGRFRLFKGRGDKKVDENKK"
     gene            complement(6436..>7945)
                     /locus_tag="AC16CB_05630"
     CDS             complement(6436..>7945)
                     /locus_tag="AC16CB_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3341982.1"
                     /translation="FEDALAKVKANLGKEYAMLIDGKDRFAAEKFEDRSPINTDMVLG
                     VFQKGTEQDAHDALAAARRAFPVWSKMKWQERVALLRKAADLIDERTFEIGAAMALEV
                     GKNRMESLGDVAETADLVRYACYQMEKSEGFRVEMGRDPLAGYTATNFSVLRPYGVWQ
                     VISPFNFPTALTGGPTGSALVTGNTVVMKPATDTPWVVRLLVECFRDAGLPDGVVNYV
                     TGPGRTLGQALIESPEVDGVTFTGSYDVGMKIFRDYAQGRYVRPIILELGGKNPAIVS
                     RNADLERAAIGIVRSAFGLQGQKCSACSRVFVEEPVYDKLVDRLVELTKKLVVGDPTE
                     RQVYMGPVINQNSYKDFKNYTEELSQAGTFLTGGKVVTDGDYAKGYFCEPTLVADVPL
                     DHRLWQHEMFLPITTVARIGSLDEAMQHANSVTYGLTAGFYGSKEETQWFFENIQAGV
                     TYANRPQGATTGAWPGFQPFGGWKGSGSSGKNAGGHYYLQLYMHEQIQTMIE"
BASE COUNT         1985 a         2305 c         1953 g         1702 t
ORIGIN      
        1 gtgcacctgt gcgtgtgaaa cttgcccatt tcgacatgga aatcccccct gcaggtgata
       61 ccatcttcga tcaaaatgtc ggcgattacc tggctcctgg ggttcatatg atgttgaatg
      121 ggccagaagt ctggcttgta gatacatcta attagcccaa taacgccgag gttcagaaat
      181 tactaaacaa catttattac atcgagatat acacaatttg aggaaatcct aaccgggtag
      241 catcaaatct tgtggatgga gaggagtgac agatgattag tcgagtatgg catggttgga
      301 caacgcatca aaacgccaca gcctatgaga ctctacttaa gagcgagatc tttacaggca
      361 tacaaagccg acagattcgt ggctacaaag gcattcatct cttccgccgg gaccttatcg
      421 atgaggtcga gttcgtgacg gtcatgtggt ttgacacatt ggaggctgta cgtgattttg
      481 caggggaaga ctacgaaacc gctgttgtcc ctccaaaagc gcgcgcattg ctctcccgct
      541 ttgatgcgcg ctcgcaacac tacgaggtaa aggctgaaat gagagtataa gcactccaca
      601 gcctggatta aatcatggat aaatgaagaa ggagatttac taatgagcga tggaaaccaa
      661 cgcaccaata cgttaatagg tttagcaacc attggaagtg tagtcacagg ggtcataagt
      721 cttttaggtg cacttatcac attgttaagt ggagagtttc tgccagcggc ggtctttctc
      781 attgccgctg cattggcgtt tggattgttc gcaatcgccc tgatcaggaa ataaatatca
      841 tccccctcta ccgctgaacc tccccagcga ttataagggt atttttgcac aacctgctat
      901 cacgtaagga caggttttac tataacccct gagctagcac ggcacaggta gacttgcgcc
      961 tggcgacccg tacgcccctg gtaggcctcg ctgagggact ggatgaacgc ctcagcttga
     1021 ctctcctcaa ccaggttaac cgtgcagccg ccgaacccgg ctccggtcag ccgcgcaccg
     1081 tagcaccctg gtaaatcgcg ggtgatatcc accagcgtgt ctaactccgg aatactgacc
     1141 tcataaaaat cgcgcaggct ggcatgacca gcgtacatca gcgcgccgaa gatctgggca
     1201 tcttgccgct gcagggcagc taccgccgac tcgacccggg ctatctccct tacgacgtgc
     1261 tcggcgcggc gtgcaatctc aggaggcagg agagcgctgt aagcagcaaa ctctggggga
     1321 gcaatgtctc gcaaggcgcg catatccggc ttatactggc gcaatagttc caccgcctgc
     1381 tcgcaagcct ggcgccgctg gttataggcc gagctggtca ggctgcggcg cacgcccgaa
     1441 tcagcgacga tgatcactgt ccccgctggc aagggtagcg attgccactc cagactgcgc
     1501 gtgtcgaagt acagtgcatg cccttccacc ccacaggcgc ttgcaaactg gtccattagc
     1561 ccacaggcta cgcccacgta atgattctcg gcgcgctgac ataagcgggc caagtccagg
     1621 ttgcctaccg accagccacc catcgcccgc catagcccag caaatgccac ttccacggca
     1681 gccgatgagc tcaagccagc acctattggt acggtcgaag aatatgccac ctgggcacca
     1741 accacctcta gcccggcttc ctgcaatgcc caggctacac ccgccggata tcgcgcccag
     1801 cctggtagcg gctcaccctc cagatccact ttggcttcca cctgatcggc ccggaatgaa
     1861 accgctttgc tcaaatccaa agctcgcagg tggataatct ggtcttcggt cggggcagca
     1921 accaaataaa cagcccggtc cagagccacc ggcagcacca ttccatcgtt gtaatccaca
     1981 tgctcgccga gcaggttcac ccgtcctggt gcgcgcactt gtaatgtcgg gcgacgggag
     2041 aatacttgtt gaaatgggat aaccagttcc atgatcttaa tcccaacgaa ctcatgctga
     2101 tggagaaaac tcttctcgaa atctactcaa ctgaaagcct atttgaccaa cataaccaac
     2161 acattccctg atacgaacag cccaattaat ccccaaatcc agatttccga acccgcgcca
     2221 gcggcgaagt acaatcctgc tgaggatagg accatcaacc caaaagcaaa cgaatggatc
     2281 tcgtaggttt tattttgctt cgaatttttc atctcactta aatactcaag atcaccccaa
     2341 tagatgtaag gatgctgatc agcaaaacca gtagcactgc ataaatcacc agttttcgca
     2401 tgacatcacc ctcccttcct gccattcccg ccgtgcttgc gcccacgacc accttcgtcg
     2461 gtgccatcac ggaagccagc gctgcccctg atgtttgccc agccaagatt atggcagcag
     2521 tgtagccaag cagctctgca gtgcgcaact gcaatgcgcc aaataccacg ttcgaattcg
     2581 tgttactacc cgtcatgaag gctcccaaag caccgatcca aggcgcaact gctggaaaga
     2641 ggacacccac cccttccgct aagccgcgcg ccaagcgatc agtcatccct gcgttttcca
     2701 tgatcactgc catggccacc atagaggcaa tacttacact ggaagacatc actcgcctta
     2761 atgtgccgtc cagaatgcgg cgcactgatc cgggtttgta taagccattt aatcgataca
     2821 ccacataggc taagatcgcc gaatacatca acaacgtccc agtgtgtcca aatacagcga
     2881 tctttcgccc tgcaccggct ggggtaataa agcccaacgc ggtgtgcaac tccggaaatt
     2941 gaacctgcac gaccacctgc cctagaaagc tcttgacccc cggggtaagc tgtacaccca
     3001 atatgaccaa aaccaaaata gcatatcccg agagcgccac cagtaaggcg cgcccatcca
     3061 aaacaccatt atctttctgg tcaccgcgat acctgcgggc aattgggaat gccatagcta
     3121 acccagctat cgaaccggcg aaggcggcga tattccataa accgctgact gccaccaggt
     3181 attggacaat gcccattacg atacctaaaa tcagaacagg caagaacaag cggcgcacgg
     3241 ctttccaatt accagtggcg tgcgcgacca tccaacccac gccaagcccg gaaatgccca
     3301 gaaaaagggc ggaaggaggc gccaaagctg tgcttggtaa cccagtagct gccattaaag
     3361 ccaggaagga agagcccaga gagccaaatg tcaccgccca gccatggcct atcgaaggga
     3421 taaccacaga agccagcggc gtaaaaccca ggccaaccag aatgggtgcg atcaccgcca
     3481 ccggcacgcc aaatccgcct acgccctgca agaacgaggc gaagatccag ccgatgacca
     3541 gtgcctgcat gccctggtca ttggttagtt gaggcagtgc ctgaccaatg gttcggattg
     3601 cacccgcctc atcagcaacg cgatacacca agaatgctgc ccagatgacg agcaagacat
     3661 ctatcgccag cagcagggct ttcgcgtggg cataagctat taactcggga cccgcgccaa
     3721 aaaacaatat ggcgatcagt aaggccacca gataacccgc accgccagcc tgcgccgctc
     3781 cccagcgcaa gccaaccatg aggaagagaa tgaggacaat aggtgtaaga gcgaggatga
     3841 atgtcatgag gaatacaggg atcgaggtcc cttgtccaat tcagttgatg tgtttatgca
     3901 gaactcttaa gatagattcc atctgggtcg agttcaaggc gcaggatgcg caactcttcc
     3961 tcagagggag gatcggtaag acgcaattgt gcagcaactt tcagatccca gccagtattt
     4021 gctttcgcct gctcgactgt acaacccgga tgcaatgccg tcagggtcaa ctcaccattt
     4081 tcatcgggct ccatgatacc caggtcggtg acgacagcct gaggcccgcc gccgcgcagg
     4141 ttggcagcct cgcgctcggc acgcccgccc aagaaacctg ccgaagtacg gaaatccact
     4201 ttctctggga agcggcgctt ttggtgcggg gtgaggatat agcaacggtt cgcccaagcg
     4261 gaaatctcca tcgagccccc tgagccgggc aggcgtacct tgggatgctg gtagtctccg
     4321 atcactgtgg cattgatatt gccaaagcgg tcgatctgcg cccctcccag aaaaccaaca
     4381 tcgatattgc cgcgctgcaa atagaaggca aagatgtcat acatgctaca caccgagagt
     4441 gccccactga ccagtgtagg atcgccaatg gagagtggca ggcgcgcagg ctgggcgcca
     4501 atcacacccg cttcgtagat catcaccagg ctgggtgcat gggtatggcg ggcaaggttg
     4561 caggccaagt ttggtagtcc aacgcccaca aagaccacat cgccatcccg caaaagccgg
     4621 gcggcattta tggttaataa ttcggaagat gagtactcta tcataatttc tcttcagagt
     4681 tttcagggga ataagggata ggggcataag gggcaggtgt ttcatgcatg attttgttat
     4741 ctcgacgttt tttgagtgta ttgattgacg cattgagcat cttacgcgtt tcattattca
     4801 aagaaattaa gttatttagt tcttttgcag agcctatccc acagtccaat gcagtattga
     4861 gccaatgatc cacctcattc gccgaacctt gagcaatcgt caaataatgg atatattcct
     4921 gacctacatg tctgccatgc ccttcagcga tgttagctcc gatcgatgtt gccgatcgaa
     4981 acaattgtct tttaatttcc tgggattgca tagttttggg ccaagaattc atgatcttat
     5041 tcagtagctg gaccacctcg cgaggtttct ggtaaatttt taagtctcta tagtccacag
     5101 ccttctccct tatcccctat cccccctgcc ttaatacttt ccataatcca ccgtggcgct
     5161 gggtcgggag ccaacctgca agcgttgatg cacttctttt ccaagcttct cccagtattc
     5221 ggcgcggtca ctcactccat acacccactc atccagataa ctctgtaccg cctctttgga
     5281 ttgggagatg cggtcccaat ccagataaaa agcattatct cgatcgtagt acccctgcgt
     5341 gtaggaggga tgtgcacaat acggcacatg gcagaccgcg tcaacaataa aacctgggat
     5401 caacgtacgg tttggatcgg agcggatgat ctcctcatcc acaatctcct ctgcggtcaa
     5461 gatcacgcga tcggcagcaa aagcggcttc cttttgctcg ccaatgatcc cccaaatatg
     5521 ggcattgcct tgggcatcgg cgcgctgcac atgaacaatc gcgacatccg gatttaaggc
     5581 gggcaccacc accacgttct gtccggaata ggggtcaacc actgtacggt agttgggatt
     5641 ggaccgctcc aaatccacag ctgcagtggg tttcatgggc ataaaaggca aaccggtcgc
     5701 tccagcctgt atgcgagaga tcatactgaa atgcgagtat tcttcccaat ccaggtttcc
     5761 ggcttcgagt tcagcccggc agatgcgcag cgatccgacg cccgggttgc ccatgtagga
     5821 aaagatgatc tttcgggcgc aacccgccgc taccacctgg tcgtaaatca agtctggcgt
     5881 ggcgcgcgcc aacacaagat cccgcttttc ctggcggatg atctcatgcc cagccgcaaa
     5941 tgggatcagg tgggtaaagc cagctgcgta taccaggtca ccgtcttgaa catagcgggc
     6001 aaccgcttcg gataaagaga gcaatttgga cataagcctc actttttatt ctcatcaact
     6061 tttttgtcgc ctcttccctt aaagaggcga aaacggccgc tgggcggcgg ggcaggacct
     6121 cgtcctatcc acatcaaata gcctccaaaa aagagcacca ccaagccata acagaagaac
     6181 gaataaatgg gctccctgac ctgatcagtc ataaaaaaga caaccaggcc gatcaaaccc
     6241 aataagacca gaaactgccc cacctgccac ttgaacatgg ttattcaccc cttcgccgga
     6301 agggagggcg gaaaacaaat agcgcaattg gagtagtgcg agcgcagagc gtccgtaaaa
     6361 ccccaattgc gcttattctc ccctccttct tgtcatccga ggaaggggca agggggaaat
     6421 aaaactcaat ccaactcact caatcatcgt ctggatttgc tcatgcatat agagttgcag
     6481 ataataatgc ccaccagcat tcttgccact ggatccggat cctttccacc caccaaaggg
     6541 ctggaagcca ggccaggcac cggtagtggc tccttgcggt cgattggcat aagtcacacc
     6601 agcctgaata ttttcaaaga accactgggt ctcttctttt gagccataaa aaccagccgt
     6661 caatccatag gtgacgctgt tcgcgtgctg catggcttcg tccaggcttc caattctcgc
     6721 cacagtagtg atgggcaaga acatctcgtg ctgccacagg cggtgatcca gggggacatc
     6781 ggctacaagg gttggctcgc agaaataacc cttggcataa tcgccatcag tgaccacctt
     6841 acctccggta aggaaggtac ccgcctgaga gagttcctcg gtatagtttt tgaaatcctt
     6901 gtaggaattc tggttgatta ctggccccat ataaacctgt cgttccgtgg gatcgcccac
     6961 caccagcttc ttggttagtt ccaccaggcg atccaccaat ttgtcataca caggttcctc
     7021 gacgaatacg cgcgagcaag ctgagcattt ttgaccctgc aagccaaagg ctgagcgcac
     7081 gatgccaatt gcggcgcgct ccaggtctgc gttgcgcgag actatggcgg ggtttttgcc
     7141 acctagctct aagataatcg ggcgcacata acgcccctgg gcgtagtcgc ggaatatctt
     7201 catgcccaca tcgtaagagc ccgtaaaggt gacgccatcc acctctgggc tttcgatgag
     7261 tgcctgacca agagtgcgtc ctggacctgt gacgtaattc accacgccat ccggcaggcc
     7321 agcatcacgg aagcattcaa ccagcaggcg caccacccag ggtgtatcgg tggcgggctt
     7381 catcacgacg gtatttcccg tcaccaaggc agacccagtt ggcccacctg ttagcgcagt
     7441 ggggaagttg aaggggctga tcacctgcca caccccgtag ggtcgtaaaa cagagaaatt
     7501 tgtggcagtg taacctgcga gcggatcgcg gcccatctcc acccggaagc cctcgctctt
     7561 ctccatttga taacaggcat agcgcaccaa gtcggcagtc tcagccacat cgccaagaga
     7621 ctccatgcgg ttcttgccta cttccaaagc catcgccgcg ccgatctcga aagtgcgttc
     7681 gtcgatcaaa tccgcagctt tgcgcagtag ggcaacgcgc tcctgccatt tcatcttgct
     7741 ccacacgggg aaggctctgc gagccgccgc aagcgcatcg tgggcgtctt gctcggtccc
     7801 tttctggaat acgcccagca ccatgtccgt attgatcggc gagcgatctt cgaacttctc
     7861 ggcggcaaat cggtccttcc catcaatgag catagcatat tccttgccca ggttggcttt
     7921 gaccttcgcc agcgcatctt caaag
//
LOCUS       DBNGSX010000177         2742 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_567578, whole genome shotgun sequence.
ACCESSION   DBNGSX010000177 DBNGSX010000000
VERSION     DBNGSX010000177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_567578"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="AC16CB_05635"
     CDS             complement(<1..251)
                     /locus_tag="AC16CB_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341983.1"
                     /translation="MRLHIVRSVEDFDSLAEEWNNLLSQSASHVPFLRHEYLATWWRT
                     LGGGEWSDGELYIVNARHADGALCGIAPLFFTKNLDGEAA"
     gene            372..2672
                     /locus_tag="AC16CB_05640"
     CDS             372..2672
                     /locus_tag="AC16CB_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341984.1"
                     /translation="MDLHFLPLVRHEGQDQAELPGLHIAVPPRRMARGRQSDRLILYF
                     TMEGNAPLVAEQQEQLIRRLVEVYYKTPGSVTAALRNVIENLNQYLLDRNLRAASTGR
                     QGLGLLTSITLRDDRLILAQSGSVAAYLLTADEVQHIVDLEPTRRGLGLGRTAPLSYA
                     QMSLSDNDVLLVSHRPPAAWNENLLTGLHGQGLESLRRHLVPAAGDELAAVLIQARPG
                     PGKTFLLHPKPLPISAEQVTPQPSEVIEEAVPAIEETPTVDEPLEMSPPPEAVTWDAP
                     PADVPPMQAAPALEDELISPPSTTPPPVSPTYPEEETPELVSPVRGARRRTRRSLALP
                     VLATISRAFGTAFSALSQSLSTLFKRMLPGEGSLPAPVMLLTAVIVPLIVVAVATVIY
                     FQSGVAGQYQGLYAQAVQAAGAAQAENDPLARERAWQTVLEKLDQADAYQVTSDTQAL
                     RDTALQAIDELNLAIRLDFQPAIIEGLPSGSQVTRIVATETDLYLLDSSTGVVVRAIF
                     AGRGYDIDKTYQCGPGAQGSQGIGPLVDIHLYPKPDGSGTALLGMDGQGNLLECSPGA
                     PPLFTLLASPPAGWAGPNAFTIHAGDAYVLDPGTKQVWVYRNGSFSTPPDLFFDQDIP
                     PLEDAIDLAAGQEDLFLLHQDGHITLCSIGSLGVVATQCTDPQPYADARPGRSGLPLT
                     PPAPFIEVRSTQPPDPSLYLLEAGTPGVYHFSLRLAYQRQLRLQMNLVTRDILETKSA
                     TAFALSPDGRVMFLAFGDEVLYAGMP"
BASE COUNT          541 a          834 c          773 g          594 t
ORIGIN      
        1 gcagcctctc catccaagtt cttggtgaaa aagagtggcg caatcccgca aagggcaccg
       61 tccgcgtggc gggcattgac aatatatagc tctccatcag accattcccc accgcccagg
      121 gtgcgccacc acgtagccag atattcgtga cgcaaaaatg gcacgtgact tgcactctgg
      181 gatagtagat tgttccactc ctcagccagg ctatcgaaat cttccacgct gcggacgatg
      241 tggagtcgca taaaatctcc gtgttttctc aaccctcagg attgagaaga accatagctc
      301 caaatttagt gggtaagcgt tcaccagttg cattttacca gtataatgtc cataggcagg
      361 ctgcaccgta tatggatctt cattttcttc ctctcgttcg tcatgaaggg caagaccaag
      421 ctgaattgcc cggattgcat atcgctgtcc cacctcgccg tatggcgcgc ggccggcaat
      481 ccgaccggct gatcctttac tttacgatgg agggcaatgc ccccctggtg gcggagcagc
      541 aagagcagct cattagacgg ctggtggaag tctattacaa gacgcctggc tcggtgactg
      601 cggcattgcg caacgtcatt gagaatctga accagtactt actcgaccgt aacctgcgcg
      661 ctgccagcac cggtcgccag ggattggggt tgctgacctc gatcaccttg cgcgacgacc
      721 ggctcatcct ggctcaatcg gggtcagtcg ctgcctatct gcttaccgcg gatgaggtgc
      781 agcatatcgt tgatctagaa cccacccggc gcggtctggg attgggacgc actgcgccgc
      841 tctcctatgc ccagatgagc ctgagtgata acgatgtgct tctggtcagc caccggccgc
      901 ctgcggcgtg gaatgaaaac ctgctgacgg gcttgcacgg tcaagggctt gagagcctgc
      961 gccgccatct agtccctgcg gcaggggatg aactggctgc ggtgttgata caggccaggc
     1021 cagggccggg caaaaccttc ctgctgcacc cgaaacccct gccgatttca gcggaacaag
     1081 ttactcctca accatctgag gttatcgagg aggctgtgcc agccattgaa gagacaccca
     1141 cagtggatga gccgcttgag atgtctcccc ctcccgaagc cgtgacttgg gatgcaccac
     1201 ccgccgatgt gccccccatg caggcggctc cagctctaga ggacgagcta atttcgcccc
     1261 cctctaccac tcccccacct gtttccccaa cctacccgga agaagagacc cccgaactgg
     1321 tcagcccggt aaggggggct cgccgccgga cgcggcgcag cttggcgctg cctgtcctgg
     1381 cgacgatcag ccgagctttt gggacggcat tttcggcgct ctcccaatcg ttgagcacgc
     1441 tctttaagcg catgctgcct ggagagggta gcctgccagc tccagtgatg ctgcttaccg
     1501 ccgtgattgt accgctcata gtggtggcgg ttgctacggt aatttatttt caaagcgggg
     1561 ttgccgggca ataccagggt ctgtacgccc aggctgtgca ggcggcgggt gccgctcaag
     1621 cggaaaacga tccgttagct agggagcgag cctggcaaac cgtgctggaa aagctcgacc
     1681 aggcggatgc atatcaggtg acgagtgata cacaggcttt acgggatacg gctcttcaag
     1741 ccatcgacga actgaacctg gccatccggc tggattttca gcccgccatc atcgaaggcc
     1801 tgcccagcgg atcacaagtc acgcgcatcg tcgccaccga gaccgacttg tacctgctcg
     1861 actccagtac aggggttgtg gtcagggcga tcttcgccgg gcgcggttat gatatcgata
     1921 agacctacca gtgcgggcca ggcgcgcagg gtagtcaagg catcggtccg ttggtggata
     1981 ttcacctgta tccaaaacca gatggctcag gcactgccct gctaggcatg gatgggcagg
     2041 gcaacctttt ggagtgcagc cccggcgctc cccccttatt cactttgctg gcctcccctc
     2101 ctgccggctg ggcagggcca aacgcgttca ccatccatgc tggggatgcc tatgtgcttg
     2161 atccaggcac gaagcaggtg tgggtttacc gcaacggcag tttttctacg ccgcctgacc
     2221 tgtttttcga tcaagacatc ccaccgctag aggatgcgat tgacttggca gccggccagg
     2281 aagacctgtt cttgctgcac caagatgggc acatcactct gtgttccatc ggtagcctgg
     2341 gtgtcgtcgc cacgcagtgc accgatcctc agccctacgc ggatgcccgc cccggccgct
     2401 caggtctgcc cttgaccccg cctgcaccgt ttattgaggt gcgttcaacc caaccgcccg
     2461 acccatcgct ctacctgctt gaggcgggga cgccgggggt atatcacttc agcttgcgcc
     2521 tggcgtatca gcgccagctc aggctacaga tgaacctggt cacccgcgat atcctggaga
     2581 ccaaaagtgc gaccgctttt gccctcagcc cagatggaag ggtcatgttc ctggcttttg
     2641 gggatgaggt gctctacgcc gggatgccgt agatttccct agtctaatat atttatatcc
     2701 atcctgctat tggcgtgttt gtgagcatct tgtcgtctct ag
//
LOCUS       DBNGSX010000178         2365 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_845388, whole genome shotgun sequence.
ACCESSION   DBNGSX010000178 DBNGSX010000000
VERSION     DBNGSX010000178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_845388"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="AC16CB_05645"
     CDS             complement(<1..294)
                     /locus_tag="AC16CB_05645"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016107.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR3341985.1"
                     /translation="MKLKPINPSSFNLLIPLSIAMHFIFPIQILVPIPLRYFGLGFIT
                     LGIILNYLSARQLKKMQTSIQFDESPARLVRDGPFRVSRNPIYLGGVTVLLGMA"
     gene            complement(335..559)
                     /locus_tag="AC16CB_05650"
     CDS             complement(335..559)
                     /locus_tag="AC16CB_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341986.1"
                     /translation="MVYFFKEGGGGGFHSGIRLYPTKGIASVVMVNSTEFDSTNFLYR
                     VEGAFFDPYKFKILRGLTPTSRAYFSLRFA"
     gene            complement(644..1882)
                     /locus_tag="AC16CB_05655"
     CDS             complement(644..1882)
                     /locus_tag="AC16CB_05655"
                     /inference="COORDINATES: protein motif:HMM:NF046778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3341987.1"
                     /translation="MKKLNIGIVGGSIAGCSAAILLGREGHNVRVFERSRSGLVGRGG
                     GIGTPGPVLEALIEQDILDSDFPHFSCSSMPFIIRTAAHERHGYSPWEMPLNLKAFHW
                     SSLWNDLRKRVPDEIYHRGYQVANAIPNDIDSVTLQFEDGSEADFDLVLFADGYQSLG
                     RQLLFPEIGLDYRGYMLWRGLLPEQEMDDSAPLGSKLPRLSYSNLPGNLAIYFVPNHD
                     GSVEEGNRIYNWAAYIPIPESELPEFMVDRDGISHSGTIPPGNMRLEEENRLKRLMQD
                     NLPDYYGEIIGKTENTYVQLIYTVRLPAYHQGRLCLIGDAGMVAQPFTGSGVFKGYNN
                     IKDMLQALKAYDRIDDALKHWGSEQVRIGNRLLALGKQMEQAFIWDSLDLATADAKST
                     EAWWKQAVTFPEEFTYAADE"
     gene            complement(2115..>2365)
                     /locus_tag="AC16CB_05660"
     CDS             complement(2115..>2365)
                     /locus_tag="AC16CB_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3341988.1"
                     /translation="MEGHARGELDGVGRWELIEKEGFTRVTYDWRVRTTKAWMNLLAP
                     LAKSAFSWNHDVIMTEGGRALARRLGVNLRGIKNETLK"
BASE COUNT          639 a          617 c          516 g          593 t
ORIGIN      
        1 agccatgcct aatagcacgg tgacgccccc caaatagatt ggattccggc taacccggaa
       61 gggaccatcc ctcaccaatc gagcgggaga ctcgtcgaat tgaatggacg tttgcatctt
      121 ctttaactga cgtgcagaaa ggtaattaag gattatgccc aaggtgatga accccagccc
      181 gaaatatctt aacggaattg gaacgaggat ttgaatcggg aagataaaat gcatcgcaat
      241 cgaaaggggt attaagaggt tgaaggaaga tggattgatg ggcttgagtt tcattatgac
      301 tttggaaaga atactgcctc aatcggtcca cccgctatgc aaagcgcaag gagaagtatg
      361 ccctgctggt cggtgttagg ccgcgcaata ttttaaattt gtatggatcg aaaaacgctc
      421 cttccacgcg atacaagaaa ttggttgaat cgaactcggt gctgttcacc atcaccacag
      481 aggctatgcc ctttgtcggg taaaggcgta ttccactatg gaaaccaccg ccaccgccct
      541 ctttgaagaa atagaccacc ccgtttgtct cttatggcag atctatccat cataactttg
      601 tatagtgggc acccccacag cttaacctac taatcgcaat ctgctattca tcggcagcat
      661 aggtaaactc ttctggaaaa gtaaccgctt gtttccacca ggcttcagtc gatttcgcat
      721 cggctgtggc aaggtcaagc gaatcccaaa tgaaagcctg ttccatttgc ttccccaaag
      781 ccaacaggcg gttcccaatc cgcacctgtt cactccccca gtgttttaga gcatcatcaa
      841 tcctatcgta ggccttgagc gcctgaagca tatctttgat gttgttatat cccttaaaaa
      901 cgcctgagcc agtaaaaggt tgagcaacca tgcctgcatc accaatcaaa cacaaccggc
      961 cctggtgata agcgggtaag cgaacagtat aaataagctg cacataggta ttctcggtct
     1021 tgccaataat ctcaccgtag tagtcgggta gattgtcttg catcagccgc tttaggcgat
     1081 tttcctcttc cagtctcata tttccgggag ggatcgtacc tgagtgagaa attccgtcac
     1141 ggtcaaccat aaactcaggt agttctgatt ctgggatggg gatatatgcc gcccagttat
     1201 atattctatt gccttcttct acggagccat cgtgattggg gacaaagtaa atcgcaagat
     1261 taccaggtag atttgaatac gatagccgtg gcaacttgct ccccaggggg gcactgtcat
     1321 ccatttcctg ttcgggtaac aaaccacgcc ataacatata gcctcgataa tccaggccaa
     1381 tctcgggaaa aagcaactga cgacccagcg attgatatcc atcggcgaat aatacgagat
     1441 cgaaatctgc ttctgagcca tcttcgaatt gcagggtgac agaatctata tcgttcggta
     1501 tggcatttgc gacctgatag cctctatggt agatttcatc cggaactcgt ttgcgcaagt
     1561 cgttccacag tgaactccaa tgaaacgctt tcaagttcag aggcatctcc caaggagaat
     1621 atccatgcct ttcgtgtgca gctgtgcgga tgatgaaagg catggatgag caagagaaat
     1681 gcggaaaatc ggaatcaaga atatcctgct caattaaagc ctccaacact ggacctggcg
     1741 tcccaattcc cccacctcgg cccaccaagc cgctgcgcga acgctcaaat acgcgcacat
     1801 tatgcccttc acgacctaac agaattgcag ccgaacaacc cgcaattgag cctccaacga
     1861 taccaatatt caatttcttc atcacttaaa actcctatcg tgtgtatgta gaacaatggt
     1921 aaaacttaac cgacgcttta ttcggggttt caaacgcaag ccaatgccga tagcaaatct
     1981 aacgagtaac tcactcgcca ggtttttgct ctttaaaaat tgtacaccat ctccaccacg
     2041 acatatagct agatggtaga gtagagcctt gtaagccatt gattgaaaag ttgaaccttt
     2101 ccaccctcat tggattattt cagggtttcg ttctttatcc ccctcaggtt cacgcccaaa
     2161 cgccgcgcca gcgcacggcc tccttcggtc attatgacgt cgtgattcca ggaaaaagca
     2221 gattttgcca gaggcgctag caggttcatc caggctttcg tggtgcgcac gcgccaatca
     2281 taagtgacac gggtgaagcc ttctttttct attagctccc atctcccaac cccatccagt
     2341 tctcctcggg catgcccttc catca
//
LOCUS       DBNGSX010000179         7655 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1121905, whole genome shotgun sequence.
ACCESSION   DBNGSX010000179 DBNGSX010000000
VERSION     DBNGSX010000179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7655)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7655)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7655
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1121905"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="AC16CB_05665"
     CDS             complement(<1..426)
                     /locus_tag="AC16CB_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR3341989.1"
                     /translation="MRILFVARAVSIHTARWISQLYGLGWDLHLFDMLGSFPHPELRD
                     ITEYSLLFPRKLQPFARGRVSYGHPFFIQHGLDPFPLSLIGYFTRRIFRNRVKRLAKL
                     IQEIKPNVIHSIEMQAESYPLLNTRRHLGGDFPVPWILSS"
     gene            complement(473..2494)
                     /locus_tag="AC16CB_05670"
     CDS             complement(473..2494)
                     /locus_tag="AC16CB_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341990.1"
                     /translation="MNSGRFPRDWITTSIALAYTGYLSYAVTHLLETPLTIRLRTLLI
                     AGWLLVVQTCALVVLLVVFRRGDRLYKLLEQGARWVQVLDRRVRGLFYLALMVSIATL
                     SYTYVSRGLMENKALRMAVLLALIVVAVQFWPFQAPRSWGGRFLITLLGGAFSLLSGD
                     YLQYAVNYPFSLHWSEGNRLYDYSLIFGKGLYQYDGNLSLPYYAPGRYGLWGIWFLIP
                     GLPISFHRFWNATLWIVPPILLGWLLARSLSRQPGVRLGVALWLGLFLSQGPIYAPIL
                     LVASLLVAFDRSKTRERYIAIGVASLYAGLSRWTWFGAAGAWGALSQICAAEVKPRQP
                     HLKRYIQIVWVIIVGSLPGILANWTQFFSPKDIAFPLSQPKLWYRLFPNSTYPLGIIP
                     GLALACGPLLVILIWLVSSRRFKLGKLQLVAIGGVMVVTLVAGLLASVKIGGGSNLHN
                     LDMFFITLALIVMLYLDQTTTQEDYESFDNPRRSDDEMQQDESSPSLMRGWPYWAKAV
                     MVVASLMLAWPYFSNVRTLKLPPQEDIQRSLDNLRLKIERYKGKGDILFLDQRQLLTF
                     EVIKDIRLLPEYEKKYLMDQAMAGNAVYFQDFHNDLANKRFALIVSEPLFMSYDNEYD
                     PFGEENNAWVKWVSEPVLCFYAPEKTYKEMRVQLLLPRTGTLDCALSHD"
     gene            complement(2491..3633)
                     /locus_tag="AC16CB_05675"
     CDS             complement(2491..3633)
                     /locus_tag="AC16CB_05675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3341991.1"
                     /translation="MRILVLIHEFPPIGGGGGQVAKDICQKLVKLGHEITLITAHTKG
                     QLRKEVIEGIQIIRQPSFRRDAAVADLLAMGAYILFSLYPALRLVRHWRPDLIHVHFA
                     VPAGALAWIVSRLTGIPYLLTVHLGDIPGGVPEKTENWFRWFYPFTPAIWRDAARVVA
                     VSNYTRGLAAKFYPVNVHVIHNGVDLEQLDPGEIRVGSPPTIVFAGRFVSQKNPVQLV
                     KILSGLKDLPWHCVMLGDGPLREEVEWQIRENGLQNRFTIPGWVHPDEVLGWFARSDI
                     LFHPSLSEGLPVVGVQALAMGLAIVASRVGGYVEIVKPDQNGYLYFPDDAAGMRSGLR
                     SLLENPIALREARLQSRRLAAHFDLQLIAQDYLSIIQEVVADLAFV"
     gene            complement(3633..4601)
                     /locus_tag="AC16CB_05680"
     CDS             complement(3633..4601)
                     /locus_tag="AC16CB_05680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3341992.1"
                     /translation="MKLSVVIPVYNEAENLPVMQKALSNCFGGQDRDWEVIYVDDGST
                     DVSLTILEGFASEEPEHTSVVSLRRNFGQTAAIAAGIDHANGDIIVLMDADMQNDPAD
                     IPKMLEKIEEGYDVVSGWRKNRQDTFITRTLPSRIANWLISTITGVHLHDYGCTLKAY
                     RREALTGFRLYGEMHRFIPAYAHAVGATIIELPVTHHPRKFGKTKYGLNRTIKVILDL
                     FTVKFLISYAHKPIYLFGGTGTLFMIISTIMLAFLFIRKLLFNTSVLQSTFFQIGIML
                     MILGVQSILMGLIAELLVRTYHESQRKPTYTVRKVLNNKRAKTIEP"
     gene            complement(4622..5608)
                     /locus_tag="AC16CB_05685"
     CDS             complement(4622..5608)
                     /locus_tag="AC16CB_05685"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR3341993.1"
                     /translation="MKINLSAFAKKQSKILRIVGTVTALALLIYVLHEQGWEEIGNAV
                     KQIDVWRLGLALLMMIISRLAVSGRWYILLRSGDAPITPKECFQITFAGLFANNFMPT
                     TVGGDVIRLAGALRLQCDTAISAASLIADRLVGMAGMLMILPVGLPRLLQIHDLRDLI
                     PTNAEVIQFSLAATPLGDLPRKIFGRLQSILHRLLSTLTVWLRQPRALLLSLLFTWIH
                     QICLFSALTLFFSGMGESISFWMVGGLYSFVYLITLIPISINGYGLQEVSMTLVFSRL
                     GGASLASSLTAALLLRTAVMVASLPGAAFVPSLLAYSRERPETKNAPQTDIV"
     gene            complement(5605..7152)
                     /locus_tag="AC16CB_05690"
     CDS             complement(5605..7152)
                     /locus_tag="AC16CB_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3341994.1"
                     /translation="MTKEFGGKWWATIAISALALNPVTIKTYSLAVSQGLTACMLIWS
                     VVCILGMQRSTWQILLGATLAGLMLMTRINMASVLPILIIFVFWRHGPRKGILATLAG
                     ILPFLLFHIIYWPHIMQFWANWFPQRITPFLDASRYTDVGEAIWVNSLPLRDRWWSFL
                     QGLRSNYLALVGLFGFSLLWLFPKQWKNTYLLQAAACISMIIVPLLILHAWASLMLNS
                     CVYCFEGYMTFFAPLCLVLIPIVYQGWKTQDKSWFNWYAGGLLIFLWSSVGYGSYRQT
                     GPMLIEIPLPRMKEGQILSGTAPLYSILEAIFGIEVKPSTWLLPTVADLVFGTILLLL
                     IWLIYRFEVIKPGFFISQGFLILSIMLIIGLVLSPTVVLAGSKMEHVCYGDVIASYES
                     VGKHLASLIPPGSSVYWEGGLSVVPLLYLPGIRIYPAQINDGYSFRIGGNAEELQRAG
                     LWISELQTRWLSEADFILVEQDRYSKAINYWTEILKPDRFNELEISPSFLPCRETRLR
                     VFQRIEQ"
     gene            complement(7482..7646)
                     /locus_tag="AC16CB_05695"
     CDS             complement(7482..7646)
                     /locus_tag="AC16CB_05695"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR3341995.1"
                     /translation="MQDPRFYRPAEVDLLVADPGNAEEKLGWQPRVSFQELIHLMVNA
                     DLRRVSAGIS"
BASE COUNT         2104 a         1917 c         1879 g         1755 t
ORIGIN      
        1 actggatagt atccaaggta caggaaaatc cccaccgaga tggcggcgag tatttagcag
       61 cggatagctc tcagcttgca tttcaataga gtgaataaca tttggcttta tctcttggat
      121 gagtttcgcc agcctcttga cacggttgcg gaatattcgt cgggtaaagt aaccaatcag
      181 ggaaagggga aatgggtcca gcccatgctg gatgaaaaat gggtgaccat aagaaacacg
      241 cccacgagca aatggctgga gcttgcgggg gaacagcaga gaatattcag tgatatctct
      301 taattcaggg tgaggaaaac tcccaagcat atcgaacaga tgcaaatccc aacccagacc
      361 atatagctgg ctgatccaac gcgcggtgtg gatactaact gctcgagcga caaataggat
      421 acgcatagaa cctcttgcaa ctcatcaatc agcacatccg caggcaacct tcttaatcgt
      481 ggctcagcgc gcaatccaat gtgcctgtac gtggcaagag taattggaca cgcatttctt
      541 tgtaggtctt ctcaggagcg taaaaacata gcactggttc cgagacccat ttcacccagg
      601 cattgttttc ttcgccgaag gggtcatatt cattatcgta gctcatgaat aacggctccg
      661 atacgattaa ggcaaatcgt ttgttggcta gatcgttgtg gaaatcctgg aaatagacag
      721 cattaccagc catagcctga tccattaaat acttcttttc atactctggc aataggcgaa
      781 tgtctttgat aacctcgaag gttagtagct ggcgttgatc caggaataag atatcaccct
      841 tgcccttata acgctcaatt tttaggcgca gattatctag cgatctttgg atgtcttcct
      901 gcggtggcag tttcagcgtt cggacgtttg aaaagtatgg ccaggcaagc ataagagagg
      961 ctacaaccat aaccgccttc gcccaataag gccaacctcg catcagcgag ggggagctct
     1021 catcctgctg catttcatca tccgatctcc ttggattgtc aaagctttca taatcttcct
     1081 gtgtggttgt ctgatctagg tacaacatga caatgagtgc cagggtgatg aaaaacatat
     1141 ccaggttgtg cagattgctg ccaccgccga tctttacgct ggcgagcaaa cctgcgacta
     1201 gggtaacgac cattacaccg ccaatggcta ccagttgcag cttacccaac ttgaaacggc
     1261 gcgaagacac tagccagatt aatatcacta gtaacggccc acaagctaaa gccaatccag
     1321 gtatgattcc caatggatat gtgctgtttg ggaacaagcg gtaccatagt ttgggttgag
     1381 agagtgggaa agcaatatct ttaggcgaga aaaactgtgt ccagttcgct aatattcccg
     1441 gcagactacc cacaatgatg acccagacta tctgtatgta gcgcttaagg tgtggttgcc
     1501 tgggttttac ttcagccgcg cagatctggc ttagcgcccc ccaggcgcct gctgccccga
     1561 accaggtcca gcgactcaaa cctgcataga ggcttgccac accgattgca atataacgct
     1621 ctcgagtttt agatcgatca aatgcgacta acaaggaagc taccagtaag ataggggcat
     1681 agattgggcc ctggctcaag aacaaaccaa gccacaacgc cacgccaagg cgtacgcccg
     1741 gttgcctgga gaggcttcgc gccagcaacc atcccaggag aataggcggt acaatccaca
     1801 aggtagcatt ccagaagcgg tggaaggaga tcggcaagcc aggaattaag aaccagatac
     1861 cccacagccc atatctacct ggtgcataat agggtagcga taggttgcca tcgtattggt
     1921 aaaggccttt accaaagata agcgagtaat cgtataaacg gttgccttcc gaccagtgaa
     1981 gagaaaacgg gtaattcacc gcatattgta gataatcgcc gctcaaaaga gagaacgccc
     2041 ctccaagaag agttatcagg aagcgtccgc cccaacttct tggtgcttgg aaaggccaga
     2101 actgaactgc tactacgatc aatgctagca agaccgccat tcgtaaagct ttgttttcca
     2161 ttaacccacg gctgacgtag gtgtagctca acgtggcgat gctcaccatc agtgccaaat
     2221 agaataaccc acggactcgc ctatccagaa cttgcaccca acgcgccccc tgttccagta
     2281 atttgtagag ccgatcgcct cgcctgaaca caaccagcaa taccaccagc gcacaggtct
     2341 ggacgaccag caaccatccg gcgatcaaga gggtacgcaa tcggatggtt aggggtgtct
     2401 ctagcaggtg tgtcactgca tagcttaaat agccggtata ggctaaagcg atgctagtag
     2461 tgatccaatc cctcggaaag cgcccgctgt tcatacgaaa gccaaatctg ccaccacttc
     2521 ttgaataatg ctcaggtaat cctgtgctat aagctgtaga tcaaagtgag cggctagtcg
     2581 acggctctgt aaacgggctt cccgtaacgc gattgggttt tccagcagac tccgcagacc
     2641 acttctcatt ccggcagcgt catcaggaaa gtaaaggtag ccgttctgat ctggtttaac
     2701 aatctcgaca taacctccga cccggctggc gacaattgcc aagcccatcg ccagtgcctg
     2761 gacacccacc acgggtaatc cctcggaaag tgagggatgg aagaggatgt cgctgcgggc
     2821 gaaccacccg agtacttcat ctggatgaac ccaacccggt atggtgaagc ggttctgcaa
     2881 cccgttttcc cgaatctgcc attccacttc ttcgcgtagg ggcccatctc caagcattac
     2941 acaatgccag ggaagatcct tcaaccctga caaaattttc actaattgta ccgggttttt
     3001 ttgagagacg aaccgcccgg caaaaacgat ggtgggtggt gatccaaccc ggatttcgcc
     3061 cgggtctaac tgttctagat ccacaccatt atggatgacg tgcacattta ccggatagaa
     3121 tttcgccgct aatccgcgcg tataattgct cactgccacc actcgggccg catctctcca
     3181 tatcgccgga gtaaaaggat aaaaccagcg gaaccagttc tcggtcttct ctggcacacc
     3241 accgggtata tcgcccaggt gcacggtcaa taaatacggg atgccagtta ggcgcgatac
     3301 aatccaggct aatgcgccag ctgggacagc gaagtgaacg tggatcaggt caggcctcca
     3361 gtgcctaacc aggcgcaaag caggatacaa gctaaaaagg atgtatgccc ccatagcaag
     3421 cagatcagca acggcggcat cgcgccgaaa ggaaggctgg cggatgatct ggatgccttc
     3481 gatgacctcc ttgcgcaatt gccccttcgt gtgagctgtg atcagggtga tttcatgccc
     3541 tagcttaacc aacttctggc agatatcctt ggctacctgt ccaccgccgc cgccaatcgg
     3601 ggggaattca tgaattaaaa cgaggatgcg cattagggct caatggtttt agcacgtttg
     3661 ttgttcaaaa ccttgcgcac agtgtaggtt ggtttgcgct gagattcatg gtaggtgcgc
     3721 acgagcagct cggcgatcaa gcccatcagg atggattgga cgccaagaat cattaacatg
     3781 atgccgattt gaaaaaaggt cgattgcaaa acgcttgtat tgaaaagcaa cttgcggatg
     3841 aacaagaaag ccagcatgat cgtgctgatg atcatgaaga gcgtcccagt tcctccaaaa
     3901 agataaatgg gcttgtgcgc ataactgatc aggaacttga ccgtgaaaag gtccaagatg
     3961 actttaatcg tgcggtttag tccatatttg gttttgccaa atttacgggg atggtgggtg
     4021 acaggtagct cgatgatggt ggctccaact gcatgggcat aagcagggat aaagcggtgc
     4081 atctctccat acaagcggaa accagtcaac gcttcgcggc ggtaggcctt gagcgtgcag
     4141 ccgtaatcgt gcaggtgcac acccgtaatg gtggagatca accagtttgc aatgcgggat
     4201 ggtaatgtac gggtgataaa agtgtcctgg cggttcttgc gccagccgct gaccacatca
     4261 tagccttcct cgattttctc cagcatcttt gggatgtcag ccgggtcgtt ctgcatgtcg
     4321 gcgtccatca agacgatgat gtctccattc gcatggtcga tgccggcggc gatggcagcg
     4381 gtttgtccaa aattgcgacg caagctaacc acagaggtgt gctcgggttc ttcagacgca
     4441 aatccttcca ggatagtgag gctgacatca gtgctcccgt cgtctacata aattacctcc
     4501 cagtctcgat cttgaccccc aaagcaattg cttaacgctt tttgcatcac tggtaggttt
     4561 tcagcttcgt tatatacagg gattacaact gatagtttca tggccatatc cactttctgt
     4621 gttagacaat atcggtttgg ggagcatttt ttgtttcagg gcgttcgcgg ctgtatgcca
     4681 gcaagctggg gacgaaagct gcgccaggca ggctagcaac catcactgct gtgcgcagca
     4741 gcagtgcagc cgtcaggctg ctggcaagcg aagcgcctcc taggcgcgag aaaacaaggg
     4801 tcatagaaac ctcctgtaag ccgtatccgt ttatagagat tgggatcaag gtgatcagat
     4861 aaacaaagct gtagagacca ccaaccatcc agaaagaaat gctctccccc atgccactaa
     4921 agaaaagagt cagcgcgcta aacaggcaga tctgatggat ccatgtaaaa agaagagaga
     4981 gaagcagagc gcgcggctgc cgcagccata ctgtcagcgt tgaaagcagg cggtgtaaga
     5041 tgctttggag acgcccgaaa atctttctgg gtagatcgcc caatggtgtt gcagccaagc
     5101 tgaattggat tacttcagcg tttgtgggaa tcagatcgcg gagatcatgg atctgcaaga
     5161 gtcgtggcag cccaactggt aaaatcatca gcatgcctgc catgccgacc aaccggtcgg
     5221 cgatgagcga agccgcactg atggctgtat cacattgtaa gcggagtgca ccagccaacc
     5281 ggattacatc tccgccaacc gttgtgggca tgaaattgtt agcaaaaagc cctgcaaagg
     5341 tgatctgaaa acactctttt ggagttattg gggcgtctcc cgaacgcaat aagatgtacc
     5401 aacgaccgga cacagccagc cgtgaaatta tcatcattaa caaggctagc cccaaccgcc
     5461 atacatcgat ctgctttacg gcattcccga tttcttccca gccttgctcg tgaagcacat
     5521 aaatcaataa agccaatgcg gtcactgtgc cgacgatgcg cagtatttta gactgttttt
     5581 tcgcaaatgc cgataaatta attttcattg ctcaattctc tggaaaactc gcaagcgcgt
     5641 ttcacggcaa ggaagaaagg aaggtgaaat ttctaattca ttaaatcgat ccggctttaa
     5701 aatttctgtc caatagttga tcgctttgga gtaacgatct tgctcaacga gaataaaatc
     5761 cgcttctgat aaccatcgcg tttgtaattc cgaaatccac aacccagcgc gttggagctc
     5821 ttctgcattc cccccaatac ggaacgaata gccatcgttt atctgtgcag gataaatcct
     5881 aattccgggc aaatatagta aaggtactac agataggccg ccttcccaat aaacagaaga
     5941 gcccggaggt attaagcttg caagatgctt gccgacgctc tcataggatg caatcacgtc
     6001 tccatagcat acatgttcca tcttggaacc cgctagcaca acggtgggtg ataatactag
     6061 accaatgatg agcataatac tcaatatcag gaagccttga cttataaaaa acccaggctt
     6121 aataacttca aagcgataga tcagccaaat caagagcaat aggattgtac caaagacaag
     6181 gtcggcgacg gtcggcaaaa gccatgtcga aggcttcact tctatgccaa agatagcctc
     6241 taaaattgag tagagcggag ctgtaccgct cagtatttgc ccttctttca tccgtggcaa
     6301 tggtatctct atcagcatcg ggcccgtctg tcggtaagat ccatagccca cacttgacca
     6361 caagaaaatc aatagacctc ctgcatacca gttgaaccag cttttatctt gtgttttcca
     6421 accctgatat actatgggaa ttagcaccag acaaagaggg gcgaagaacg tcatatagcc
     6481 ttcgaaacag tatacacaag aatttagcat aagcgaagcc catgcgtgca atattaaaag
     6541 agggacgatt atcatgctta tgcaggcagc cgcttgcaga aggtatgtgt ttttccattg
     6601 ttttggaaac agccataaaa ggctaaatcc aaacagtcca acgagggcta aataattgga
     6661 tctaagtcct tgtaagaaac tccaccagcg gtcccttaat ggcagagaat tcacccatat
     6721 agcttctcca acatccgtgt aacgcgatgc atccaagaat ggtgtgatcc tttggggaaa
     6781 ccaattggcc cagaactgca tgatatgggg ccaatagatg atatgaaaga gtagaaaagg
     6841 taatatacct gcaagcgtcg cgaggatacc cttccgaggg ccatggcgcc agaatacgaa
     6901 aatgatcaag attggaagga cagatgccat attgatgcgc gtcatcagca taagccctgc
     6961 taacgtcgcg cctagcaaga tttgccatgt tgaacgctgc atacccagga tgcaaacaac
     7021 actccatatc agcatacagg cagtcagccc ctgggaaaca gccaagctgt aggtcttgat
     7081 cgttacagga ttcaatgcta gcgcactaat tgcaatggta gcccaccact tccccccgaa
     7141 ttcttttgtc aagatccaca atccgatcaa catcaggagg gcaagaatta tagcaaaata
     7201 gcgggcggtg cgcaaacccg gttcgatcaa gacctggaag gcgccaggaa tgagaaacga
     7261 caagggcatg tggttgctcc atggcccgta atcctggtaa ggcgtatatc gaccagtggc
     7321 gaacaagtag cctttataga ggtatgcccc ttcatctaag atggattcct ggctatgtgc
     7381 atatatcact gcttgtacca agtaggcgaa ccccgccagg acggctattg cttctgctgt
     7441 ccagcgagaa gaggaaatac gccgtatggt ggattttagg ctcatgaaat tcctgcggat
     7501 acccgtctca aatcggcatt gaccattaaa tgaattaatt cctggaagct cacacgaggt
     7561 tgccagccta gcttctcttc ggcattgcct gggtcggcta ccagcaggtc tacctcggct
     7621 gggcggtaaa agcgcgggtc ctggattacg tagtc
//
LOCUS       DBNGSX010000180         2573 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_984330, whole genome shotgun sequence.
ACCESSION   DBNGSX010000180 DBNGSX010000000
VERSION     DBNGSX010000180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_984330"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="AC16CB_05700"
     CDS             complement(<1..1252)
                     /locus_tag="AC16CB_05700"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR3341996.1"
                     /translation="MIENNNTNIIQMIVVVLIIAVAALAGMTSLAMPAVVPASAPAEE
                     FSAERAMGHIRAISQEPRPTGSQGHTQARDYIISQLEALGLSPEVQQTTAVMPWGSKI
                     DATIVYNIIARIPGTNSSGAILLDAHYDTMPTTPGAVDCGFCVATLLETARALQAGPP
                     LQNDVILLFTDKEESSYYTSGYTGATAFIEQHPWASEVRQVLNFDGMGRTGPSIMFQT
                     GPHSGWLVREWGRVASQPVVQPWFSEIFRLTGQGTDLSPFADAGIGGLSFFSLFENTI
                     YHTVLDNPESIDPRSVQHHGSNALSMTHLLGDLDLTEAQSPGDVVYFTLVRGLLVSYP
                     ATWTIPLVLMAGVLLAGIAVIGFRRRQLTLRGLLRGLSASLLSVIAIPGLATGLWIGI
                     FQLHGAYQSMFTFGRVYNAPLYYWA"
     gene            complement(1551..2480)
                     /locus_tag="AC16CB_05705"
     CDS             complement(1551..2480)
                     /locus_tag="AC16CB_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3341997.1"
                     /translation="MEHLLIAYDIVVIIIGFAVLSNAVVWALRTGQDDLGNFSIVYGL
                     YTLMVVLLVLKKYLFVNVAAYSGQTWYYILLIYQVLEFAVIVAALHYFLDAHQFRFGR
                     KVTAVFLLLMFVCIGLVISPFGLTFDANKNIIRYGTGFWTAIGWNAVTFTCAVVLGYW
                     LLGRVWKTKKRTFTLGILIFASVGYAESLITFFREFGTTTSVLKPERGFLYSSIPYAL
                     YGIFLIVYFLRYPVSAPVGNEEPSREFLSKYGITQREREIIIKVMQGKSNEDIARELF
                     ISLATVKTHLHNIYQKIGIDSRYELVARVRLGQ"
BASE COUNT          644 a          651 c          676 g          602 t
ORIGIN      
        1 aggcccaata gtacagggga gcgttataga ctctcccaaa ggtaaacata gactggtatg
       61 ctccgtgcag ttggaagatc cctatccaca ggccggtggc caggccggga attgcaatga
      121 cactcaacag gctggccgac aaaccacgga gaaggcctcg cagggtgagt tgccttcgcc
      181 gaaaaccgat aactgcaatc ccagccaaca gcacgccggc cataaggacc agcgggatgg
      241 tccacgtggc cgggtagctc accagcagcc cgcgtacgag cgtaaaatag acaacgtcgc
      301 cgggactctg ggcctcagtc aggtccagat cccccaggag gtgcgtcatc gacagggcat
      361 tcgagccgtg gtgttgcacg ctgcgcgggt cgatgctctc agggttgtcc agcacagtgt
      421 ggtaaatcgt gttttcaaat agggaaaaaa agctcaaccc gccgatcccg gcgtcagcaa
      481 acgggctgag gtcggtgccc tgtccagtga gccggaatat ttcgctaaac cagggctgta
      541 ccaccggctg tgaggcgacc cgaccccatt cccggacaag ccagccgctg tggggaccgg
      601 tctggaacat gatagaaggt ccggtgcggc ccattccatc aaagttcaac acctgccgga
      661 cttcgctggc ccacggatgc tgttcgatga aagctgttgc ccctgtgtaa ccactagtgt
      721 aataagacga ttcctctttg tcggtgaaaa gcagtatcac gtcgttttgc agaggtggac
      781 cggcttgcag agcacgggct gtttcaagca gcgtggcgac gcagaaaccg caatcgacgg
      841 cgccgggcgt cgttggcatg gtgtcatagt gcgcatccag caatatcgct ccactggagt
      901 tggttccggg tatcctggca atgatgttgt acacaattgt ggcatctatt ttgctacccc
      961 aaggcatcac tgcggtagtt tgctgtactt caggggaaag ccccaatgct tcaagctggg
     1021 agatgatata gtcacgtgcc tgcgtgtgtc cctgcgagcc agtggggcga ggctcctggg
     1081 agatagcgcg gatatgtccc attgcccgtt cggcggagaa ctcctcggcg ggtgcgctgg
     1141 ccggcacgac agccggcatt gcgagcgagg tcatgcctgc aagcgctgca acagcgatga
     1201 tcagaacgac tacaatcatc tggataatgt tcgtattatt gttttctatc atttcatagc
     1261 tctttttctg tttaggatgg atggtttgtt ttagtggttc gtatctacta gctatgtttt
     1321 ttgactcttt ttgtgttgaa gattgatgat ttggttgcat agctgatatc tccttatctt
     1381 cggcaacaga tttctcctga cgggcgcgat ccaatcatga gattgatgct gccattctcc
     1441 acccatttca gagatatgag aatcgatctt cggatgtatt tctaggaaaa ttccctacaa
     1501 ctttggattg agattgacga ttttccccct gcgttcagct tgcgagcggg ctattgaccc
     1561 aagcgcaccc tggcgaccag ttcgtagcgg ctgtcgatgc cgatcttctg gtagatatta
     1621 tggaggtggg tcttgaccgt cgccaacgag atgaacaatt cccgggcaat gtcttcgttg
     1681 cttttccctt gcataacctt gatgataatt tctcgctccc gttgcgtgat cccgtatttt
     1741 gacagaaatt cccgggaggg ttcttcattt ccgacgggtg cggatacggg ataacgcagg
     1801 aaataaacga tcaggaaaat tccataaaga gcgtacggaa tggaactata caggaacccg
     1861 cgttcaggtt tgaggacact ggtcgtggtg ccgaattcgc gaaagaaagt gatcagggat
     1921 tccgcgtaac ccactgaagc gaaaattaaa atacctagag taaaggtcct ttttttagtt
     1981 ttccaaacgc ggcccaataa ccaatatccc agcacgacgg cacaggtaaa cgtgacagcg
     2041 ttccacccga tggcggtcca aaatcctgtt ccataccgga tgatattctt attcgcgtcg
     2101 aaggttaacc cgaatgggga gatgaccaac ccgatgcaga caaacatcaa cagcaggaac
     2161 accgcggtca ccttccttcc aaagcggaac tggtgcgcgt cgagaaaata atgcagggct
     2221 gccacaataa cggcaaattc aaggacctga taaatgagca aaatgtaata ccaggtttgg
     2281 ccagaatatg cggcaacgtt gacgaacaaa tattttttca gaaccaggag taccaccatc
     2341 agcgtgtata ggccgtagac aatgctgaaa ttgcccagat cgtcttgccc tgtccttaaa
     2401 gcccaaacga ccgcgttgga aaggacagcg aaaccaatga tgataaccac aatatcgtaa
     2461 gcgatcagca ggtgttccat aattgccact catcatatct gattttgaac gaaataaaca
     2521 aggtcacgcc attttcaaga ttgatcttca aaattccacc tgccgaaaaa ttg
//
LOCUS       DBNGSX010000181         9109 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_984503, whole genome shotgun sequence.
ACCESSION   DBNGSX010000181 DBNGSX010000000
VERSION     DBNGSX010000181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9109)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9109)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9109
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_984503"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..126)
                     /locus_tag="AC16CB_05710"
     CDS             complement(<1..126)
                     /locus_tag="AC16CB_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR3341998.1"
                     /translation="MSKQKFERTKPHMNVGTIGHIDHGKTTLTAAITKYSQILGQG"
     gene            complement(174..2246)
                     /gene="fusA"
                     /locus_tag="AC16CB_05715"
     CDS             complement(174..2246)
                     /gene="fusA"
                     /locus_tag="AC16CB_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR3341999.1"
                     /translation="MARKLQLERYRNIGIIAHIDAGKTTTTERVLFYTGKTHRIGSVD
                     DGTTVTDWMEQERERGITIVSAAVTAEWKGYQINIIDTPGHIDFTAEVQRSLRVLDGG
                     IVIFDAVQGVEPQSETVWRQADRYRVPRICFVNKMDRVGASYERTIKMVRERLGANPI
                     AVQLPIGVESAFRGAVDLITMQALIWEDNLGKEPQQSEIPVDLRPQAEEMRARLIEQV
                     AETDDELTMKYLEGLDISEDELKLALRRAVIAGVATPVFCGSSLRNKGVQPLLDAVVD
                     YLPSPADVPAVTGMHPRTEETHERPPKDDAPLSALVFKIVTDPYVGRLAYLRVYSGKL
                     SQGSTVLNSTKDQRERIGRLIRMYADRREDINEVLAGDIGAILGLKNSFTGDTLCDMS
                     SPILLENISFPEPVISVAIEPKTTADQDKMSEALRKLSEEDPTFRVRGDEETGQTIIA
                     GMGELHLEVLVDRMLREFRVQARVGRPQVSYRETITLPVGRAECRYVKQTGGRGQYGH
                     VILEIQPGESGSGISFKNDIIGGVIPKEYISSIEKGVREAAEGGVVAGYPVTDVMIRL
                     YDGSYHEVDSNEMSFKMAGSLAFKEGIQRGAPVLQEPIMKVEVVVSEEFLGDVIGQLN
                     ARRGEILGIEVRPGNAQAVRAMVPLAEMFGYATDLRSATQGRGVFSMEFDHYARVTEN
                     VARAIIGA"
     gene            complement(2323..2793)
                     /gene="rpsG"
                     /locus_tag="AC16CB_05720"
     CDS             complement(2323..2793)
                     /gene="rpsG"
                     /locus_tag="AC16CB_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIR3342000.1"
                     /translation="MSRRYKPEKFDITPDVRYNSVNVQSFINRVMRSGKKSVAATLIY
                     NAMDTIQERTKRSPLEVFDQAFKNVSPVMEVKPRRVGGATYQVPMEVSPQRRFALASR
                     WIIAAARDRSGKTFSEKLAAELIDAANNTGSAIRKREETHKMAEANRAFSHYRL"
     gene            complement(2811..3233)
                     /gene="rpsL"
                     /locus_tag="AC16CB_05725"
     CDS             complement(2811..3233)
                     /gene="rpsL"
                     /locus_tag="AC16CB_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIR3342001.1"
                     /translation="MPTTNQLVRKGRKSKKVKSKAPALLYTFNSMKQRRLRQEKGAPQ
                     KRGVCTVVRTMTPKKPNSALRKIARVRLTNGIEVTAYIPGEGHTLQEHSVVLVRGGRV
                     KDLPGVRYHIVRGTLDTGGVNDRQQGRSKYGSKRPKTK"
     gene            complement(3426..7340)
                     /gene="rpoB"
                     /locus_tag="AC16CB_05730"
     CDS             complement(3426..7340)
                     /gene="rpoB"
                     /locus_tag="AC16CB_05730"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR3342002.1"
                     /translation="MATKLPSKSYARIDVNLSLPDLIEVQIESFERLKREGLADLFFE
                     LSPIESYNKGMKLYFPSNSKESKEWGLTYWFDEPKHNIEECVERDLTYARPLYVSVLL
                     AGPDVPEPIKQDIFLGDFPEMTEKGTFIINGTERVVVSQLIRSPGVYFEAPIDRSTGR
                     PLAMAKLIPDRGAWMEFETRKTDYLTLRFNRKRIVPVTIFLRALAAVNDSLPDSPLKH
                     GTDEELLELFGDVDNNPDRMFIASTLSQEPEWDLSSGISIAEAALLEFFKRMRPGDPA
                     TLDNAREFLEEQLFDQRHYDLERVGRYKLNQKLELHEHVPITHRMISKWDIVRLVRRM
                     IMINNIMESPDDIDHLGNRRAKTVGELIQNKLRIGLRRMERVIKERMSIRDQDQLSPI
                     TLINIRPVVAALREFFGSSQLSQFMDQTNPLAELRHKRTLSALGPGGLRRERAGFDVR
                     DVHHSHYGRICPIETPEGPNIGLIGRLASFARVNEYGFIETPYRRVRRTLASNDPKIE
                     GRFLREDLIHPKKGTVIAKSGDRITPKIAKSVAKLDLPEIAIVPFVTNEVVYLSADVE
                     DRYVIAQANAPLNENKEFVRRRVSSRFHSTFIFSESTNIDYMDVAPHQIVGISAALIP
                     FLEHDDANRALMGSNMQAQAVPLLRPDVPQVSTGMEYDAARDSGQVVIAEEDGEVVSV
                     TGRSIVIRSDDGKLRTYTLRKYQRSNQSTCIDQRPAVVKGQIVQRNDVIADSSSTESG
                     ELALGQNVMVAFLSWEGGNFEDAILISERLVQDDAFTSIHIEKYEVESRDTRLGPEEI
                     TRDIPNVGEEAIKDLDDQGIIRIGAEVGPNDILVGKISPKGEKELTPEERLLRAIFGE
                     KSRDVKDTSLRMPHGERGKVVDVKVFTREDNTDLSPGVDMMVRVSVAQRRKITAGDKM
                     AGRHGNKGVVSKVVPAEDMPFLEDGTPVDIILNPLGVPGRMNIGQVLETHLGWAASRL
                     GFRAISPVFDGAREDEIEAELARAWMINQSWKETGEVAWEWLNQQEYDPITIMDDDEV
                     RRLYLEQWLGERGYDVYQLISDSKYARRSVLREWLRDNGFDPDQILYFGAVESQEDVN
                     PKPGLDENAVNACLRLWLKGRGENTKKIADDQLRSKAEEVTLRDRIPSPVLGKQILRD
                     GKTGEAYHQPVTVGVMTMLKLHHLVEDKVHARSTGPYSLVSQQPLGGKAQFGGQRFGE
                     MEVWALEAYGAAYTLQEMLTVKSDDVQGRVKTYEAIVKGEPIEEPSIPAAFRVLVKEL
                     QSLGLAVEAVTEAGEVIKFGKDEERSKPPRLSTGLLGLGEDF"
     gene            7587..8507
                     /gene="era"
                     /locus_tag="AC16CB_05735"
     CDS             7587..8507
                     /gene="era"
                     /locus_tag="AC16CB_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIR3342003.1"
                     /translation="MEEEIFSTNYHSGFVAVMGKPNVGKSTLLNALLGQKVAAVSPRP
                     QTTRKQQMGILTTDSAQVIFVDTPGVHRPRYKLGEAMNQEALSILGDCDLILWIVDAS
                     QMPDEDDQMLAEQIARLNRPVETLVLLNKIDLLEQEGLSERQAAYASFLPQAHQHTIS
                     ATRGDGLDTLLNDIIDHLPQHPPYFPEDQLTDLYEREIAADLIREASLLILRDEVPHG
                     IAVRIDQFTERGETGAYIEATLLVEKETHKGIVIGQGGSMLKEIGSRARAEIEKMSGR
                     KVFLRLRVKVRKNWRNDDRVLKSFGYTKRN"
     gene            complement(8636..>9109)
                     /locus_tag="AC16CB_05740"
     CDS             complement(8636..>9109)
                     /locus_tag="AC16CB_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342004.1"
                     /translation="AQPPLAGGLGLLTVVALPVVVIALTITILLIPVSLLVIVLFGLA
                     WIFGLIGLGLELGKRLAELTKQEWAPAVSSGVGTLVLILVLNGVREVIPCIGWIPSAL
                     AGLLGLGAVLLTRFGSQAYQPQGVGAQIPAPPSAPQPPEAPMPPQPETDEETSSS"
BASE COUNT         2167 a         2543 c         2247 g         2152 t
ORIGIN      
        1 tccctgtcct aagatctgag agtatttggt gatcgccgcc gtcagcgtgg tcttgccgtg
       61 atcgatgtgc ccaatcgtcc ctacgttcat gtgcggcttc gtgcgctcaa atttctgctt
      121 ggacataatt gcgctccttc tgcaatgatg aaagtaatct agttatggcg ttgtcaagcg
      181 ccaataattg cccgagccac attctcggta acgcgggcat aatgatcaaa ttccatagaa
      241 aaaacaccac gaccttgtgt cgcagagcgt agatcggtgg cgtaaccaaa catctccgcc
      301 agtggaacca tggcgcgtac tgcctgggca ttgccagggc gcacctcaat gcccaggatt
      361 tcacctcggc gagcgttcaa ctggccaata acgtctccca gaaattcctc agagacgacc
      421 acttctactt tcatgatcgg ttcctgtaat acaggagctc ccctctggat accctctttg
      481 aacgccaacg acccggccat cttgaaagac atctcattcg agtcaacttc atgatatgaa
      541 ccatcataca agcggatcat cacatcagtc accgggtagc cagcgactac gccaccttca
      601 gcagcttcgc ggacgccctt ctcgatactt gagatgtatt ccttcgggat aacaccacca
      661 atgatatcgt tcttgaaaga gataccgctc cctgattcac cgggttggat ctcgagaatc
      721 acatgaccgt attgacctct gccaccggtt tgtttgacat agcggcactc tgctcgccct
      781 accggaaggg tgatggtctc gcggtaagat acctgtgggc gaccgacccg tgcctgtacg
      841 cgaaactcac gcagcatcct gtcaaccagc acttccaagt gcaactcgcc catgcctgca
      901 ataatcgtct ggcccgtttc ttcatcgccg cgcacccgaa aagtaggatc ctcttcggaa
      961 agcttacgca gggcttctga cattttatcc tggtcggcag tggtttttgg ctcaattgcc
     1021 accgaaatca ctggctctgg aaaagaaata ttttccagca agatcgggct ggacatatcg
     1081 cacaaagtat cgccggtgaa tgaattcttc aagcccaata ttgcgccgat atcgcccgcc
     1141 aacacttcgt tgatatcttc ccgtcgatca gcgtacatac ggatgagccg accaatgcgc
     1201 tcacgttgat ccttggtgga attcaaaacg gttgaaccct gactgagctt gccagagtaa
     1261 acacgcagat aggccaatcg acccacatag ggatctgtca caattttaaa gaccaatgcg
     1321 ctcagcggag cgtcgtcttt gggtggccgt tcatgcgttt cctctgtgcg aggatgcatg
     1381 cctgttaccg caggcacgtc tgccggtgag ggcaggtaat ctaccaccgc atctaggaga
     1441 ggttggacac ctttgttgcg caacgaactc ccgcaaaata ccggtgtggc aacaccggcg
     1501 atcaccgctc tacgcaaagc tagtttgagc tcatcttcac tgatatcgag accttccaga
     1561 tatttcatgg tgagttcatc gtcagtctcg gcgacctgct caatgaggcg cgcacgcatt
     1621 tcttcagctt ggggacgcaa atcgacggga atttcagatt gctgcggctc cttgcccagg
     1681 ttatcttccc agattaatgc ctgcatggtt atcaggtcca ctgcgccccg aaaagcagat
     1741 tcgacgccaa tcggcaactg cacagcaata ggattagcgc ccaaacgctc gcggaccatc
     1801 ttaatcgtgc gctcgtaaga tgcgcccacg cggtccattt tatttacgaa gcaaattcgt
     1861 ggcacgcggt agcgatcagc ctggcgccac actgtctcgc tttgcggctc gaccccctga
     1921 acggcatcga agatgacaat gcctccgtcc aagacacgca gagaacgctg cacttcggcg
     1981 gtgaagtcaa tatgaccagg ggtatcaata atatttattt gataaccctt ccactctgcc
     2041 gtgactgcag ccgagacaat agtgatacca cgctcgcgct cttgctccat ccaatcggta
     2101 acggtggtgc catcatccac tgagccaatg cgatgtgtct tgcctgtata gaacaagacg
     2161 cgctcagtcg tggtggtctt gcccgcatcg atatgagcaa tgatcccaat atttcggtat
     2221 cgttccaact gaagctttcg tgccatagtt actataatta caatcagtca tcaagattca
     2281 gcggtcattc atcagatgag gctgaccgct gaaagtaaaa tactataaac gataatgcga
     2341 aaaggcgcga ttggcttccg ccattttatg ggtctcttcg cgtttacgga tagccgaacc
     2401 agtgttattg gcggcgtcga tcagctcagc ggctaacttt tcagagaaag tcttgcccga
     2461 gcgatccctt gcagctgcga taatccagcg tgaagctaaa gcaaaacgtc gctgcggaga
     2521 tacctccatc ggcacctggt aggtcgcccc accaactcgg cgcggcttga cttccattac
     2581 cggagataca tttttaaaag cctggtcaaa tacctccaac gggctacgtt tggttcgctc
     2641 ctggatggtg tccatggcat tgtagatcaa agttgcagca acgcttttct taccactccg
     2701 catcacgcgg ttaatgaagg actggacatt aacgctgtta tagcgcacgt caggagtaat
     2761 atcaaatttt tcgggcttgt aacgtcgaga cataatatca ctacctatcc ttactttgtc
     2821 ttgggtcgct tggaaccata cttcgagcgc ccttgctggc gatcattcac accgccagta
     2881 tccagggtgc cgcgcacaat gtgataacgc acgccgggca gatccttcac acgaccaccg
     2941 cgtaccagga caactgagtg ttcctggagc gtatgacctt cgcctgggat ataggcggtt
     3001 acttcgatac catttgtcag gcgcacgcgt gcaatcttgc gcaaggcaga gtttggtttt
     3061 ttcggcgtca tagtacgtac cacagtacac acaccgcgct tctgtggcgc gcccttttcc
     3121 tggcgaaggc gacgttgctt cattgaatta aatgtataca acaaagcagg agccttgctc
     3181 ttaaccttct ttgacttgcg gcctttgcgt actaattgat ttgttgtagg cacctttgcc
     3241 tccgaaaagt ttccaactaa acaacttcta tccattcaga gaggccatcc gcttgcttga
     3301 tggcctctct gaaattcact cgtaaacatg ccaggcgggg ataattgccg cctgccttat
     3361 ccatgggcaa cgaacaacca ttctaccatg cgggatagtg agcgtcaagg ccattattcg
     3421 attatctaaa aatcctcacc cagccccagc aagccagtag aaagccttgg cggcttgctt
     3481 cgctcctcat ccttaccgaa tttaatcacc tctccggctt cggtcaccgc ttctaccgcc
     3541 agccccagac tctgcagttc tttaaccaga actcggaatg cggctggtat gctgggttct
     3601 tcaattggct cacccttcac gatagcttca taagtcttca cacgaccttg aacatcgtcg
     3661 gatttaacgg tgagcatctc ctgaagcgta tacgcagcgc cataggcttc aagcgcccaa
     3721 acctccatct caccaaatcg ctgcccgccg aactgcgcct tgccacctag aggctgttga
     3781 gataccaagc tgtatggacc agtggagcga gcatggactt tgtcttccac caggtgatgc
     3841 agcttgagca ttgtcatcac acccaccgtt accggttggt ggtacgcctc gccggttttt
     3901 ccatcgcgta gaatttgctt gcctaggaca ggcgagggga tgcggtctcg caatgttact
     3961 tcctctgctt tgctgcgaag ctgatcatcg gcgattttct tggtgttctc acccctccct
     4021 ttgagccaga ggcgcaggca ggcattgacg gcattctcgt ctagaccagg tttcgggttg
     4081 acatcttcct ggctttccac ggccccaaaa tataggatct ggtcagggtc aaaaccgtta
     4141 tcgcgcagcc attcgcgaag gacagagcgg cgagcatatt tggaatcaga gatcaattga
     4201 tatacatcat agccgcgctc gccaagccat tgttcaaggt acaacctgcg cacctcgtcg
     4261 tcatccatga tcgtgatcgg atcatactct tgctggttca accactccca tgctacctcg
     4321 ccagtctcct tccacgactg attgatcatc caggcgcgag ccaactccgc ttcaatctca
     4381 tcttcacgag cgccatcgaa gacaggcgag atggcccgga aaccaaggcg gcttgcagcc
     4441 caccccagat gagtctccaa cacctgcccg atattcatac gcccgggcac accaagcgga
     4501 ttcaagataa tatctacagg ggtaccatcc tcgaggaatg gcatatcttc tgccggcacg
     4561 accttggaca ctacgccttt gttaccgtgg cgtcctgcca ttttatcccc agcggtaatc
     4621 ttgcgccgct gcgcaacgga gacacgcacc atcatatcca cacctgggga tagatcggtg
     4681 ttgtcctcgc gggtgaagac cttcacatcc actaccttac cgcgctcgcc gtgcggcatt
     4741 cgtaacgaag tatcttttac gtcgcgggac ttttcgccga agatggcacg cagcaggcgc
     4801 tcttctgggg taagttcctt ctcacccttg ggtgagatct tgcccactaa aatgtcattc
     4861 gggccgacct ccgccccaat gcggataatg ccttgatcgt caaggtcttt gatggcctct
     4921 tcgccgacat tgggaatatc gcgggtgatc tcttctggac ctaggcgcgt gtcccgtgat
     4981 tcgacctcgt acttctcgat gtgaatggag gtgaaagcgt catcctgtac caagcgctcc
     5041 gagatcagga tggcgtcctc gaagttcccg ccttcccagg agaggaaagc caccatcacg
     5101 ttctgtccca acgcgagctc accgctctca gtggaggatg agtcagcgat gacatcgttg
     5161 cgctgcacaa tctggccttt gaccaccgcc ggacgttgat ctatgcatgt gctctggttc
     5221 gatcgctggt atttgcgcag agtataagtg cgcagcttac catcgtcgga gcggataacg
     5281 atagagcgtc cagttaccga gacgacctca ccgtcctcct cagcgatgac cacctgccca
     5341 gagtctcgag cagcgtcgta ttccatgccg gtggaaacct gaggcacatc tgggcgcaac
     5401 agaggcaccg cttgcgcctg catgttcgaa cccatgagag cgcgattggc gtcgtcatgc
     5461 tccaagaatg ggatcagcgc agcgctaatg ccgacgatct ggtgcggggc aacatccata
     5521 taatcgatgt tggtcgactc tgaaaagatg aaagtcgagt ggaaacgaga agacacccgc
     5581 cgccggacaa actccttgtt ctcattcagg ggagcgttcg cttgggcgat tacatagcga
     5641 tcttctacat cggcagacag atacaccact tcattggtca caaacggtac gatagcgatt
     5701 tcaggtagat ccaacttggc aacagacttg gctatctttg gggttatacg gtcgcctgat
     5761 ttcgcaatta cagtgccctt ctttgggtgt atcaagtctt cgcgcagaaa acgcccttca
     5821 atttttggat cattggaagc cagcgtccgg cgcacccgcc tgtaaggtgt ctcaataaac
     5881 ccatactcat tgacccgagc gaatgacgcc aggcggccaa tcaaaccaat gttggggcct
     5941 tctggggttt caatcgggca gatacgacca tagtgggagt ggtgtacgtc tcgcacatca
     6001 aagccagctc gctcgcgtcg caaaccgccg ggacctaaag ccgacagggt acgtttatgg
     6061 cgcagctctg ccagcgggtt ggtctgatcc atgaattggg atagctggct ggagccgaag
     6121 aattcgcgca gagccgcgac taccggacgg atattgatga gtgttatcgg cgacagttga
     6181 tcctggtcgc gaatggacat gcgttctttg atcacccgtt ccattcggcg taatccaatg
     6241 cgcaacttgt tctggatcag ttcgcccacc gttttggcgc ggcgattgcc cagatggtcg
     6301 atatcgtccg gcgactccat gatgttattg atcatgatca ttcgccgcac cagtcgcacg
     6361 atatcccact tcgatatcat gcggtgggta atgggcacgt gctcatgcag ttccagcttc
     6421 tggttcagct tatagcgacc gacacgctcc agatcataat gacgctggtc gaacaactgc
     6481 tcttccagaa attcgcgggc gttatctaaa gtcgccgggt caccaggacg catgcgcttg
     6541 aagaactcga gcaaagccgc ttcggcgata ctaataccgc tagagagatc ccactctggt
     6601 tcctgagaga gggttgaagc gatgaacatg cgatcgggat tattatccac gtcgccaaag
     6661 agctccaaca gctcctcatc cgtgccgtgt ttcaacggtg aatcgggcaa actatcattg
     6721 acagctgcca aggcgcgcag aaagatcgtc actggcacaa tgcgcttgcg gttgaatcgc
     6781 aatgtcagat aatctgtctt gcgcgtctcg aactccatcc atgcgccccg gtctgggatg
     6841 agttttgcca tagccaatgg gcgaccagtg gaacgatcga tgggtgcctc aaaatagaca
     6901 cccggagaac gaatcaactg ggagacaaca acccgctcag tgccgttgat aatgaaggtg
     6961 cccttctcgg tcatctccgg gaagtcgccc aagaagatgt cttgctttat cggctcggga
     7021 acatctggcc cggccagcag cacagagaca taaagaggtc gagcatatgt cagatcgcgc
     7081 tcaacgcatt cctcgatgtt gtgtttgggt tcatcaaacc aataagtcag cccccattcc
     7141 ttggattctt tgctattgct ggggaaatat aatttcatcc ccttgttgta agactcaatc
     7201 ggtgataatt cgaaaaacag gtcagcaagc ccctcccgct tgaggcgctc gaatgattct
     7261 atttgtacct cgatcaaatc gggtagagag agattgacat cgatgcgagc gtatgacttg
     7321 ctaggcaatt tagtcgccat attttctcct ggataccata caaacaatgt cctaaaagcg
     7381 taaaaagagg aacgttaagg ccttacttgc cttacgctac tctcattcgt tttacaccat
     7441 gaaaccatat aatttttcgt cttcaggtga taaaagccca gtagacaaaa gtaaattata
     7501 tcaaaaccat cgcagagagt caatcaaatt tggctttttt tcaagatatt cgtattaatt
     7561 cccctttttc ttgataaaat gatgtcatgg aagaagaaat tttctcaacg aactaccact
     7621 ccggcttcgt ggctgtcatg ggcaagccca atgtgggtaa atccaccctg ctgaatgcac
     7681 ttctgggaca aaaagtggct gcggtttctc cgcgccctca gactacccgc aaacagcaga
     7741 tgggcatact gacaacagac agcgcccagg tgatcttcgt agatacaccc ggcgttcacc
     7801 gcccgcgtta caaactaggt gaggccatga accaggaagc cttgagcatt ctgggggatt
     7861 gcgacttgat cttgtggatt gtcgacgcca gccagatgcc cgatgaggat gatcaaatgc
     7921 tggccgagca gattgcccgg ttgaaccggc cggttgagac ccttgtacta ttgaataaga
     7981 tcgacctact ggaacaggaa ggcctttccg aacgtcaggc agcctatgcc agctttttgc
     8041 cccaagcaca ccagcacact atctcagcca cacgcggcga cgggctggac acgctgctca
     8101 atgatatcat cgaccatctg ccacaacacc ccccttactt ccccgaagac cagctgactg
     8161 acctgtatga gcgcgagatt gctgcagact tgatacgcga agcgtcgctg ctcatcttgc
     8221 gcgatgaggt gccacacgga atcgctgtac gcatagatca atttaccgag cgtggtgaga
     8281 ccggagccta cattgaggcc accctgctcg tcgaaaaaga gacccacaag ggcattgtaa
     8341 ttggtcaggg gggcagtatg ctcaaagaga tcggcagccg ggcaagagct gagatcgaga
     8401 aaatgagcgg gcgcaaagtc ttcctcagac tgcgggttaa agtgcgcaaa aactggcgca
     8461 atgacgatag ggtcttgaaa tcttttgggt ataccaagcg gaattgagcg aaatttgtga
     8521 tggcaagacc aaatatttga gagagctaac aacaaacccc tccctgttat cttgggaggg
     8581 gtttgttgtc acccactatc ttgagtaagc gtttagcaat cagcgaatat gcccgtcacg
     8641 agcttgaggt ctcctcatcc gtttccggct ggggaggcat gggcgcttct ggtggctgcg
     8701 gtgcgctggg aggcgcgggg atttgcgctc ccacgccctg cggctggtaa gcctgtgacc
     8761 caaagcgggt gagcaagaca gcgcctaacc ccaataagcc agccagggca gaaggtatcc
     8821 agcctatgca tgggataacc tcccgcacac cattaagcac caatatcaac accagggttc
     8881 ccaccccaga tgaaactgca ggagcccatt cttgcttggt gagctcagcc agacgttttc
     8941 ccaactcaag gccgaggccg atcaggccaa atatccaggc tagtccaaat aatacaatca
     9001 ctaataggct aaccgggatc agcaagatgg tgatcgtcaa ggcaattacc accaccggta
     9061 aggcaactac tgtcagcaaa cccaggcctc ctgccaaagg gggttgagc
//
LOCUS       DBNGSX010000182         5100 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_915248, whole genome shotgun sequence.
ACCESSION   DBNGSX010000182 DBNGSX010000000
VERSION     DBNGSX010000182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_915248"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1824)
                     /locus_tag="AC16CB_05745"
     CDS             complement(<1..1824)
                     /locus_tag="AC16CB_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter family
                     protein"
                     /protein_id="HIR3342005.1"
                     /translation="MEEATTSFAPLLLVVFLAFLVPLLLGRFKSIRLPIVVGEILAGI
                     LIGRSGLGWVNGEDPVLEILSEFGFVFLMFLSGMEIDFSSLGNLPNSNTPRASENKGW
                     GPIQIGGLSFLLTLALSVVIGFVLVQLGLARNLWMVALIMSTTSLGIVLPVLKERGLS
                     VGRYGQTILISALIADFATMLLITILVAVVSHGLNLEILLVGVLFVVFGIMYRLGIQF
                     FNRLPAIRKVINDLSHATSQFKVRAAFTMMLIFVALSEFLGVEIILGAFLAGAIISVL
                     RTPEDKDLTHQLEAVGFGFFIPIFFIKIGAGFNLGAIIESPQYLLLAPLLLLAALIVK
                     FVPSLLFTRIFSRREALAAGALLSSRLSLIIAASAIGLRLGLISEAMNAEILLVAVVT
                     VTLGPLLFTRLLPPSEQIEPQPVIVVGAGQLGLEVANRIRTHNEPVYLVDLDQDQVSA
                     AIRRGFEAQVGDLENPDKTLVRKLDQAKALICTYTDPELSYKVCSLARSVFGIDAVVA
                     DVPSPNDIPRFEQLDVTPVNAALDRATLLELHVRNPDMYMLLTRLKDSKEVVEIDCNN
                     PACAGKALRQLSFPGDVLALALRRHGELIVPHGDTRIELGDH"
     gene            2200..2583
                     /locus_tag="AC16CB_05750"
     CDS             2200..2583
                     /locus_tag="AC16CB_05750"
                     /inference="COORDINATES: protein motif:HMM:NF013089.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HIR3342006.1"
                     /translation="MFRMNLGSFRPLLGSRENLLILILLVINLVFNVVSNAGFRLSAG
                     SATLKDFISWQVIGNLAGFITVLTLTGLLRYIPLHIAFPVTTGLAIIGVQLIAGRWFF
                     GEVISVERWFGTILVVIGILFLTWL"
     gene            2574..3167
                     /locus_tag="AC16CB_05755"
     CDS             2574..3167
                     /locus_tag="AC16CB_05755"
                     /inference="COORDINATES: protein motif:HMM:NF046921.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkoP family protein"
                     /protein_id="HIR3342007.1"
                     /translation="MAVTLRAMVRNFDAWVRQGRGVFEFSQDPGCLLRIQLATARIEL
                     DLPDRVVSPGDAVLLLHLWNERLLQFPPQGPDLAWAKPMQRQFVYSLRMVATCLKESS
                     ELAQVSAVGGVTVLAGLGTENGGSRLLKRLGFKLIPYTHPLGTFGEFWENFYSWVLIW
                     TYNPSALRYRSLWGLKRIEFWMSVRAFLERFGYIAYP"
     gene            3232..4575
                     /locus_tag="AC16CB_05760"
     CDS             3232..4575
                     /locus_tag="AC16CB_05760"
                     /inference="COORDINATES: protein motif:HMM:NF024919.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HIR3342008.1"
                     /translation="MRKQGCLISLIALPTTCLLCVGVYFLPPVQARLGWRVDNWLADL
                     RRSLAPEGQVVFVPKEQNEQVEAIVQATLQALLQSSPTTGADMPAQNPIANLTPTALP
                     GEPTATLEPPPTSTVTPTPIPEKMILEGIRYQRQTFNNCGPANLAMALSYWGWQGDQR
                     ETRAYLRPNLDVDDKNIMPDEMVRFVEIYTGLKALTRVGGDIDLLKRFIAAGFPVVVE
                     KGHHPPDDWWMGHYMVINGYDDDNSRFVAQDSLIQADAPLPYDEVTPWWRNFNYVYLV
                     IYSPERESEVYAILGANADIAYNYQEAAQRARDEIAVLDGRDLYFAWFNLGTNLVALG
                     DYAGAAQAYDQAFAINADLPEKQRLYRMLWYQIGPYEAYYQVGRYGDLLDLGNATLAW
                     VGKPVLEETYYWMGLAREATGDMDKAIQNFKRAAELNLNYEPPRLALQRLGVDAP"
     gene            4593..>5100
                     /locus_tag="AC16CB_05765"
     CDS             4593..>5100
                     /locus_tag="AC16CB_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342009.1"
                     /translation="MRKKITKLAIAIPFFLLLMAFAYQLPPIHSRLSWRLDELKTRIK
                     YVLNPPDEAVFVPAGGTPQPVSTSTPTSSPSPTSTLPGPTETPLPSPTSTITPTPLPE
                     WVKLDGVKYEDQHGRWNYCGPANLSMALTFWGWEGNRDVVGKAVKPNDKDKNVMPYEM
                     QDFVAEQAP"
BASE COUNT         1196 a         1348 c         1368 g         1188 t
ORIGIN      
        1 gtgatcgcca agctcgatgc gcgtgtcacc atgtggcacg atcaattctc catgccttcg
       61 cagtgcgagc gcaagcacat cccccgggaa gctaagttgg cgcaaggctt ttccggcgca
      121 ggccggatta ttacaatcga tttctacaac ttctttgctg tccttcagcc gggtgagcag
      181 catgtacata tccggattgc ggacatgcag ttctaatagc gtagcgcgat ccagggcggc
      241 atttacgggt gtgacatcca gctgttcaaa gcggggaata tcgtttggac tgggcacatc
      301 ggcaaccact gcgtcaattc cgaagactga ccgggccagt gagcacacct tataactaag
      361 ttcagggtca gtataggtac aaattaatgc cttggcttga tccaactttc taaccagggt
      421 tttgtctggg ttttctagat cacctacctg agcctcgaag ccgcggcgga tggcggcaga
      481 cacttggtcc tggtcaagat cgaccaggta tacgggctcg ttgtgggtgc gtatccggtt
      541 agctacctcc agtcctagct gtccagcacc gaccacaata acgggctgtg gttcgatctg
      601 ctccgagggc ggaagtaacc gcgtgaataa cagcggtcct agcgtgacgg ttacaaccgc
      661 aaccagcaaa atttctgcat tcatggcttc actgatcaag cccaaacgca ggccgatcgc
      721 cgaggcggcg ataattaaag atagacgcga tgataacaag gcaccggcgg cgagtgcctc
      781 ccgcctcgag aatatgcgag tgaacagcag agagggaaca aacttgacaa tcaacgctgc
      841 cagcagaagc aagggtgcca ggagcaggta ttggggggac tcgatgatcg cacccaggtt
      901 aaatcctgcg ccgatcttga taaagaagat ggggataaag aatccaaaac cgactgcctc
      961 caattgatgg gtcagatctt tatcctctgg tgtgcgcagt acagagataa tcgcccctgc
     1021 caggaaagcg cctaggataa tctccacccc tagaaattca gacagggcaa cgaaaatcag
     1081 catcattgtg aatgctgccc gaaccttaaa ttgggatgtg gcgtggctca ggtcgttgat
     1141 tacctttctt atcgccggca agcgattgaa aaactgaatg cctaggcggt acattatccc
     1201 aaagacaacg aataatacac cgaccagaag gatttccagg tttaatccat gcgagactac
     1261 agctaccagg atggtgatca agagcatggt ggcaaaatcg gcaatcagcg cagagataag
     1321 gatcgtttgc ccgtaacgcc caacgcttaa gccacgttcc ttcagcactg gcaaaacaat
     1381 gcccagagag gtagttgaca tgatgagtgc taccatccac aggttgcgcg ccagccccag
     1441 ttgaacaagg acaaacccga tgaccacaga gagcgccaag gttaaaagga agctcaggcc
     1501 gccgatttgg ataggccccc aacccttgtt ctccgatgcc ctcggggtat ttgaatttgg
     1561 tagattgccc aggctggaaa aatcaatttc cattccagaa agaaacatga ggaagacgaa
     1621 accaaattca ctcaagattt ccagcactgg atcttcaccg ttcacccaac ccaatccact
     1681 gcgcccgatc agaatacccg ccagtatttc accgacgaca attggcaagc ggatcgattt
     1741 aaagcgtcca agcagcaagg ggacaagaaa ggcgagaaat acaaccagga gtaaaggggc
     1801 aaaggatgtt gtggcttctt ccatactaat tttgattccg tatcattttc attaggaccg
     1861 attcgtctct acaatagtat agtatattcc tgtactggca gaacaccccc caattctact
     1921 tgattattct gaattctatt gctggctatc aaatataaaa gcatgccagg cacgaattgc
     1981 tccttgttct cttcgcgatc tcacgagcac aagtattggc tgccatcttg tgccgtgatt
     2041 ggtatgactt gtctctcata aatcattccg cgctaatccc atattatgaa gagtgagttt
     2101 gctgaaaagc ctacgtcaca gccgcgaaat agtgatagat ttaaggtagc tgggaaggta
     2161 acgagttttt ggaattaagt ataggtgtgg aggtgtgtca tgttccgcat gaatcttggc
     2221 tcctttcgcc cgcttctggg ttcccgcgaa aatttactca ttttgatcct gctggtcatc
     2281 aacctggtat tcaatgtcgt ctcgaatgct ggatttagac tctctgcagg aagcgcaacg
     2341 ctgaaagatt tcattagttg gcaagtgatc ggtaatctgg ctggcttcat cacagtactg
     2401 accctgaccg gtctgctgcg ctatattccc ttacatattg cctttccggt cacgacaggt
     2461 ctggcgatca tcggcgttca attgatagca ggcaggtggt tctttggcga ggtgatttcg
     2521 gttgagcgct ggtttggcac gatcctggtg gttattggca ttctgtttct cacatggctg
     2581 tgaccttgcg cgctatggtc cgcaattttg atgcctgggt gcgtcagggt cggggtgtat
     2641 tcgagtttag ccaggaccct ggatgcctgt tgcgcattca gcttgcaacg gcgcgcatag
     2701 aactggattt gccagatcgg gtcgtgtctc cgggtgatgc agtgctttta ttgcacttgt
     2761 ggaatgagcg tttgctccaa ttccctcccc agggccctga cttggcatgg gccaagccca
     2821 tgcaacgcca gtttgtgtac tcccttcgaa tggtggctac ctgcctgaag gagtcgtctg
     2881 aactggctca ggtgagcgcg gtgggaggtg tgacggtgct ggcaggcctt ggcacagaga
     2941 atggtgggag ccgcttgctc aagcgtttag ggttcaagtt gataccttat actcacccat
     3001 tggggacttt cggcgaattc tgggagaatt tctactcctg ggttttgatc tggacgtata
     3061 accccagcgc attgcgatat aggagcttgt ggggattgaa gcgtatcgag ttttggatgt
     3121 ctgtgagagc gtttcttgaa agatttggct atattgcata cccataggga ggaattaata
     3181 atatctcatc tataaaaatc cttacctgtc gccgatgata taatcgcgcc catgagaaag
     3241 cagggctgcc tgatctccct aattgctttg cctaccacct gcctgttgtg cgtgggtgta
     3301 tatttcctgc cgcctgtgca ggcgcgcctc gggtggcggg tggataactg gcttgccgac
     3361 ctgcgccgct ccttagcccc tgagggtcag gtggtcttcg tgccaaagga gcaaaacgag
     3421 caagtggagg ccatcgttca ggctaccctc caagcgctgc tccaatcgtc gcccaccacg
     3481 ggtgcagata tgcctgcgca aaatccgata gccaacctga ccccaacagc cctgcctggt
     3541 gagccgacag ctacactgga gcccccccca acatcgaccg tcacaccaac ccctatccca
     3601 gagaagatga tcttggaagg cattcgctac cagcgccaga ctttcaataa ctgcggccca
     3661 gcgaacctcg ctatggcgct ctcgtattgg ggctggcagg gtgaccagcg cgagacgcgc
     3721 gcctacctgc gccccaacct ggacgtggat gataagaata ttatgccgga tgagatggtg
     3781 cgttttgtcg agatatacac cgggctcaag gcgctgaccc gcgtgggcgg cgatatcgat
     3841 ttgctcaagc gtttcatcgc cgcgggattc ccggtagtgg ttgagaaagg ccaccacccg
     3901 ccggatgatt ggtggatggg ccactacatg gtcatcaacg ggtacgatga cgataatagc
     3961 cgcttcgtag cccaggattc tctcatccag gccgacgcac ccttacccta tgatgaggtg
     4021 acgccctggt ggcgtaactt caattacgta tatctcgtta tctactcgcc ggagcgggag
     4081 agcgaggttt acgctatcct tggtgcgaac gcagatatag catataacta tcaagaggca
     4141 gcccagcgag cccgtgacga gattgctgta ctcgatggca gggacttgta ctttgcctgg
     4201 ttcaacctgg gcacgaatct ggtggccttg ggcgactatg ctggcgcggc acaggcatat
     4261 gaccaggctt ttgccatcaa tgcagaccta ccggagaagc agcggctgta ccgcatgctg
     4321 tggtaccaga tcgggccata cgaggcctat taccaggtag ggcgctacgg ggatttgctc
     4381 gatctgggta acgccaccct ggcctgggtg ggtaagccag tcctggaaga gacgtactac
     4441 tggatgggtc tggcgcgcga ggccaccggc gatatggata aagccatcca gaatttcaaa
     4501 agggcggctg agctcaacct gaattatgaa cccccgcgcc tggcgctaca acgcctgggt
     4561 gtggacgcgc cctgattagt tggtgagttc tcatgcgaaa aaagataacc aaactggcga
     4621 ttgccatccc cttttttctt ctgttgatgg ccttcgctta ccaattaccc cccatccaca
     4681 gccgcctgtc gtggcggctg gatgagctga agacgcgtat caaatacgtc ctcaacccgc
     4741 cagatgaggc ggttttcgtc ccggcgggcg gcactccaca gcctgtcagc acgtcaaccc
     4801 ctacatccag tccttcccca acctccacct tgcccggccc tacggaaacc cccctgcctt
     4861 ctcccacctc cacgatcacg ccaacgcccc tacctgaatg ggtgaagctg gacggcgtga
     4921 agtacgaaga ccagcacggg cgctggaact actgcgggcc agccaacctc tctatggcgt
     4981 tgactttctg gggctgggag ggcaaccgag atgtagtcgg caaagctgtc aagcccaacg
     5041 ataaagataa gaatgtcatg ccctatgaga tgcaggattt tgtggctgag caggctcctg
//
LOCUS       DBNGSX010000183         1985 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_291224, whole genome shotgun sequence.
ACCESSION   DBNGSX010000183 DBNGSX010000000
VERSION     DBNGSX010000183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_291224"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            800..>1985
                     /locus_tag="AC16CB_05770"
     CDS             800..>1985
                     /locus_tag="AC16CB_05770"
                     /inference="COORDINATES: protein motif:HMM:NF024627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSLREA domain-containing protein"
                     /protein_id="HIR3342010.1"
                     /translation="MNTKYIRHLLLRYLLVCTILLFALSPFGGALAEGPTTYVVNTVY
                     DVDDGVCNKAHCSLREAINLANVHPGADTIEFKIRGGPPFTIQPSSALPEITDPVTID
                     GTTQPGFHGNPIVELDGSNAGEGEPEVGISGLVITAGSSTVKGLVINRFSGEGILMTT
                     NGGNTIQGNFVGTDVTGMLALSNTLRGIHVAGGSSGNIISGNLISGNARAGVLLTDTE
                     GNQVQGNFIGTDINGTSPLANDVGVKIMGAAKNVIGGTEAGARNLISGNAFYGVFIAT
                     EGTTGNIVQGNYIGTDVTGSIATIPAGPPVGEYTLTVMPETCPTYDAETDFDGFVSES
                     NDPSAPWSGTSVHFFGSDVCIGREMLGGASLIFSYKLEFAVITQLTSIAVSGANFNGP
                     DNV"
BASE COUNT          489 a          509 c          510 g          477 t
ORIGIN      
        1 gggatctggg atggcttggt gacctgggag agggtccgca cctatccgtc tctgccaatt
       61 tccaaaattc accaacctct tatgttacat aaaccttcgg aaaaatcata cttgacacga
      121 aaatgaattc tgctatgatg agatcaatca aacataccct gtataagcag ggaaaatagc
      181 acaagaagct ctcatctaag ctggataata tcaagcacca atctctatgc ctatgcaggg
      241 atggtgcttt tttgttttgg ggctaattag aattacacgt ctgtatctac catttttcgg
      301 aattcaccaa ccgcttgtgt tatagaaacg cgaggaaggc gatattgaca atcaaaatta
      361 aactatccat gatcaccaat attattacgt aacttcgagc ggcgaaaacc caatatgtct
      421 catccggtcg ttggttgggg aggagtaggg tttaagatga tcttagagcg aaatcggcct
      481 tttaatatgg tgggtttcgt atttattatg gttggcatcg cggggagtat ttacctttac
      541 catctacagc attactcccg gtcttttttt ggcacagtgt acggtaggta agccttgtga
      601 acatgcgatt gggtcgcgta tctatttgtc tatctattgc tagtttcagt acgaaggagg
      661 ctcaagatta aaacagtatc agggttaaat ccgaattctc gcctaacccc gattcagcta
      721 cctcgaggac ctcgtgtccg gcataggtat taccataacg ttcaaatttc tctcattcat
      781 ctcatgatgg acgaataaaa tgaacactaa atatataaga caccttttgt tgcggtattt
      841 gttggtttgc acgatcctat tgtttgcgct ttcgccattc ggtggtgctc tcgcagaagg
      901 acccaccact tacgtcgtca acacagtgta tgacgttgac gatggagtct gcaacaaggc
      961 ccactgcagc ctgcgcgagg cgatcaacct cgccaacgtc catccgggtg ccgacacgat
     1021 agaattcaaa atccgcggcg gtccaccatt cactatccag ccctcgtccg cccttccaga
     1081 gatcaccgac ccggtcacca tcgacgggac cacccagcct ggctttcacg gtaacccgat
     1141 cgtcgagctg gatgggagca atgcaggcga gggggagcca gaggtgggaa tcagcggact
     1201 cgtgatcacg gctggcagca gcacggttaa gggcctggtg atcaaccgct tctcaggcga
     1261 agggatctta atgaccacga atggtggcaa taccatccag ggcaacttcg tcggcacgga
     1321 cgtgaccggc atgctcgctc tgagcaatac ccttcgaggc atccacgttg ctggtgggtc
     1381 atccggcaac atcatctccg gcaatctcat ctccgggaac gcgagggcgg gcgtccttct
     1441 cacggatacg gaaggaaacc aggtccaggg caacttcatc ggcacagata tcaacggaac
     1501 gagcccgtta gcaaatgacg ttggcgtcaa aataatggga gcggccaaaa acgtgatcgg
     1561 tgggacagaa gccggcgcac gcaatctcat ttcggggaac gccttctatg gtgtattcat
     1621 agccacggag gggaccacgg ggaacatcgt gcagggcaac tacatcggaa cggatgtgac
     1681 cggcagcatt gcgactatcc cggctggccc acctgtgggg gagtacacgc tgacggtaat
     1741 gcctgagacc tgccccacat atgatgcaga gacggatttt gatggtttcg tctcagagtc
     1801 caatgatcca tcggcgccct ggtcagggac ttctgtgcac ttctttggct cggacgtgtg
     1861 catcggccgc gagatgctcg gtggagcctc gctaatcttc agctacaagc tggagttcgc
     1921 tgtgatcacc cagcttacct cgatagccgt ctccggggcg aacttcaatg gccccgacaa
     1981 cgtcc
//
LOCUS       DBNGSX010000184         4900 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1330335, whole genome shotgun sequence.
ACCESSION   DBNGSX010000184 DBNGSX010000000
VERSION     DBNGSX010000184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4900)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1330335"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..2501)
                     /gene="gyrA"
                     /locus_tag="AC16CB_05775"
     CDS             complement(15..2501)
                     /gene="gyrA"
                     /locus_tag="AC16CB_05775"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR3342011.1"
                     /translation="MEIGNIQQVDIDDQMRAAYLDYAMSVIVARALPDARDGLKPAHR
                     RILYAMHDMGLRSNSAYKKSARIVGEVLGKYHPHGDAAVYDTMARLAQDFSMRYPLVD
                     GQGNFGSIDGDAPAAMRYTEARLAPIAEEMLVDIDKDTVDFTTNFDDSLEEPVVLPAR
                     LPNLLLNGASGIAVGMATNIPPHNLGELASALIYLIENYDKIDDIPVEELIKFIPGPD
                     FPTGGVIVGSEGIWQAYSTGRGRIVLRGLAHIEEMSGGRYRIVVTEIPFQLNKSSLIE
                     RIAELVREGKLDEISDLRDESDRRGMSIIIELKRSAQPKKVLNQLFKYTPLQSTFGVQ
                     LLALVDGEPRLLSLKRALQIFVEHRRVVITRRSQFELQKARARAHILDGLLIALANLD
                     DVIQTIRSSPDADTARERLMFRYLLSELQAQAILDMQLRRLAALERQKIEEEHKKVLD
                     NIAYLEDLLANPGKILDVIKSDLSEISGKYGDQRRTHIAHDANEDFSEEDLVPDEAVL
                     ISITQRGYIKRVMAKAFRAQGRGGRGVTGHATREEDEVLMLVPARTLNTLLFFSDRGK
                     VYSEKAYQIPDADRTGRGIPIVNVLSLDPGETVTAMVAVPDFDAAEYCIMATRDGRVK
                     RVVLSEFSAVRPSGLIAITLDEGDELGWVRLTHGGDEVILVTEQGQALCFSEDQVRPM
                     GRMAAGVNGIKLGKNDKVASMEVVEPDGDLLVVTVGGFGKRTPMKDYPRKRRATGGVH
                     TIDKKALGKVGVVTAARAVQEADDLTIISAGGVVLRTRVQAISRSGRATRGVVLMNLE
                     EGDFVASLARIAAADLRRVGADNGGNAGND"
     gene            3067..>4900
                     /locus_tag="AC16CB_05780"
     CDS             3067..>4900
                     /locus_tag="AC16CB_05780"
                     /inference="COORDINATES: protein motif:HMM:NF049288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7507 domain-containing protein"
                     /protein_id="HIR3342012.1"
                     /translation="MNRVKVKTITVHPGRRLLWVSLGILAITAFVLSLISTIAIAAPI
                     LLVDDAGPDDEPGQKDLNFLTIDYETIAPDIAVTWGWDDTKWSGNNTGDACTLFDTDS
                     DGFANYSLCITVDQAGSYLDTRLYSCDADSRSDRCGGSSEIPTFSSVGSASIQAVDPF
                     GTSGRANNDCSSVVAGACLTNDTVAELTVDIDDVGSGSAKLINVCSYPSQQPNSDPSD
                     CVITPNAGFLTIVKVANPNDGTSFVFNASEPSAETPPISNWTINGSGSVPLISYLPTT
                     TPTLDLSEAVPAGWQLDSASCEIAGNGATGTFNGNTITNLEIRSGLETTCTFTNSLAL
                     GTLTLVKVVDNLGESGPGYLGVGDFPLTIDSVSTTSGTPVSVTPGDHAIAETPQSGYS
                     VGTWTCDDGTSGTAGSSTATVNVSSGENVTCTMTNTLIATPALELVKSATPSTYDSVG
                     DVISYSYVMTNTGNVTLIAPFTVDDDKATVTCPGTPTSLAPGEGITCSASYTISQPDL
                     DAGSVKNTAQGHGYYGTTPVDSNFDDETVTAVPDPVISIVKTGTWVDGNGDGYAQPGE
                     VISYTFAVKNEGNVTLHDVTVTDPLPGMSAISCPKTTLAVGEPMT"
BASE COUNT         1150 a         1418 c         1262 g         1070 t
ORIGIN      
        1 cgattgattg gttatcaatc attgcccgca ttgccaccat tatccgcgcc cactcgacgc
       61 aagtccgccg cggcgatacg cgctagggaa gccacaaagt cgccttcctc tagattcatc
      121 agcaccacac cgcgcgtcgc tcgcccagag cgcgaaatag cctgcaccct cgtgcggagg
      181 acgacaccac cggcggagat gatggtcagg tcgtcggctt cctgcactgc acgcgccgct
      241 gtaacaacgc ccactttacc taaagctttt ttatcgatgg tgtgcacacc accagtggcg
      301 cgcctcttgc gtgggtagtc cttcattggt gtgcgtttac caaagccacc aaccgtcacc
      361 accagcaagt cgccatctgg ctccaccacc tccatactag ccaccttgtc atttttaccc
      421 agcttgatgc cgttcacccc tgcggccatc cgccccatcg gtcgaacctg atcttcagag
      481 aagcataacg cttgtccctg ttcggtgacc aaaatcactt catcaccccc gtgcgtcagg
      541 cggacccagc cgagctcgtc accctcatcc agtgtgatag cgatcaaacc agagggacgc
      601 actgcgctaa attcggagag cacaactcgc ttgacgcgcc catcgcgcgt ggccatgatg
      661 cagtattcag cggcgtcgaa atcgggtaca gcaaccatgg ctgtcaccgt ttcccctgga
      721 tctaaggaaa gcacattgac aatagggata ccacgcccgg tccggtcggc gtctgggatt
      781 tggtacgctt tctctgaata aacctttcca cggtcagaga agaagagcag cgtgttcaga
      841 gtccgagccg ggactaacat caggacctca tcttcctcac gggtggcatg accggtaaca
      901 ccgcgcccgc cgcgcccttg cgcccggaag gccttggcca tcacgcgctt gatgtagcca
      961 cgctgggtga tactgatcag cacagcctcg tcaggaacca agtcctcttc ggagaagtct
     1021 tcgttggcat catgggcaat gtgcgtgcgc cgctgatcgc catatttccc ggatatctcg
     1081 cttaggtcgc tcttaatgac gtccaggatc ttcccggggt ttgccagcaa gtcttccaga
     1141 taggcaatgt tgtccaatac ttttttgtgt tcttcttcaa tcttttggcg ctccagtgca
     1201 gccaggcggc gcaactgcat atccaggata gcctgcgcct gcagctcgct caagagatag
     1261 cggaacatca gacgctctct ggctgtatca gcgtcaggcg agctacggat ggtctgaatc
     1321 acatcatcca gattcgccaa ggcgatgagc aatccgtcca ggatatgggc gcgggcgcgg
     1381 gctttttgca actcaaactg ggagcggcgg gtaattacaa ctcgccggtg ctcaacaaag
     1441 atctgcaagg cgcgcttgag cgatagcagg cgaggctcgc catccaccaa ggcgagcaac
     1501 tggacaccga atgtggactg caacggggtg tacttgaaga gctggttgag tacttttttg
     1561 ggctgggcgc ttcgcttgag ctcgatgatg atgctcatgc cccgccggtc ggattcatcg
     1621 cgcaggtcag aaatttcatc tagcttgcct tcacgaacca gttcggcgat acgctcgatc
     1681 aaggaagatt tgttaagctg gaaaggaatc tcggtgacca caatgcggta acggcctccg
     1741 ctcatctcct cgatatgagc tagaccacgc aacacgatgc gcccgcgacc agtgctgtag
     1801 gcttgccaaa tgccctcaga tccaacaatc acgccaccgg tggggaaatc tgggccgggg
     1861 atgaacttta tcagttcttc caccgggatg tcgtcaatct tgtcgtaatt ttcgattaga
     1921 tagatcaagg cgcttgccag ctcccccagg ttgtgcgggg gaatattggt cgccataccc
     1981 acggcgatgc cggaggcacc gtttagcagc aggtttggca ggcgcgccgg cagaacaacc
     2041 ggctcctcca gcgagtcatc gaagttggtg gtgaaatcga ctgtgtcctt gtcgatatcg
     2101 accagcatct cctcagcgat tggcgccagg cgcgcctcgg tatagcgcat agcggcaggt
     2161 gcgtcaccat cgatggaacc gaaattgccc tgcccgtcca ccagcggata acgcatagag
     2221 aaatcctgag ccagccgcgc catggtatcg tacaccgcag cgtcaccatg cgggtgatac
     2281 ttgcccagca cctcgccaac tatacgggcg gattttttat aggcgctatt cgaacgcaag
     2341 cccatatcgt gcatggcata aaggatgcgc ctgtgggcgg gtttcagtcc atcgcgggca
     2401 tctggcaaag cgcgcgcaac aatcacgctc atggcatagt ccaggtatgc ggcgcgcatt
     2461 tgatcgtcga tatctacctg ttgtatgtta ccaatttcca ttaaaaagat atccttctat
     2521 cagaaaaagt gcaaaaccgg acaaacagct taactataaa attatacctc tccaacagac
     2581 tatttgagct tcgatatatg gcgtgccatt gagtagctct atgatgtgcc acgaaaatat
     2641 tggccggatt ggctgattaa agagcgatca gatcatcggc aacctgaccc ccatcacaaa
     2701 actaaataca gtgatctatc acgccatatt ccagagtgtc tgtttctcca atctggggat
     2761 cgcacattgc cagcaacaga atactgcaat tctacgaaac agcgggtgca ttgcgcgtaa
     2821 atgcgataga tcataaggca gactcatgca gattttattg agattccatt tcttaaggac
     2881 aaagatgatt tcttacagac atctgactcc tgtaataaaa tctcattact atcgttatac
     2941 ttaacaagat tgttaggtgt tttaatagat tcataatctt atttattgga atcgaagatc
     3001 cagagatcaa aatgggtcgc taatcctttt cgaagaaatg aatagttgaa aaattaagga
     3061 gtatttatga accgcgtaaa agtcaaaact atcactgttc accctgggcg tagactgctc
     3121 tgggtatctt tgggtatact ggcaataaca gcgtttgtac ttagcctgat atcgacaatc
     3181 gcaatcgccg cccccatatt actcgttgac gacgccggcc ccgacgatga acccggtcag
     3241 aaggatctca atttcctgac cattgactac gaaacaatcg cgccagacat cgccgtgacc
     3301 tggggctggg acgacaccaa gtggtctggg aacaacaccg gcgacgcctg taccctgttc
     3361 gacaccgaca gcgacggatt tgccaactac tcgctgtgca tcacagttga ccaggcaggt
     3421 tcctatctcg atacccgcct gtattcttgc gacgcggaca gccggtccga tcgctgtggt
     3481 ggatcaagcg agataccaac tttctcgtcg gtaggaagcg cctcaattca ggcggttgac
     3541 ccctttggaa caagcggacg cgcgaataac gactgttcaa gtgtcgtcgc tggagcgtgt
     3601 cttactaacg atacagtggc ggaactgacg gttgacatcg acgacgttgg tagtggctcc
     3661 gccaaattga ttaacgtttg tagctacccg tcgcagcaac caaactcgga cccatccgac
     3721 tgcgtcatca cgccaaatgc tggtttcctg accatcgtta aggtcgcaaa tccaaatgac
     3781 gggacctcgt tcgtattcaa cgcctctgag ccttcagcgg agacccctcc aatcagcaac
     3841 tggacgatca acggcagcgg ctcggtgccg ctgatctcgt acctaccaac gaccacgccc
     3901 acgctagacc tgagcgaagc cgtgcccgcg ggctggcagc ttgattcggc gtcctgcgag
     3961 atagcgggca acggcgctac gggcacgttc aatggaaata caatcaccaa tctcgagatc
     4021 cgctcaggcc tcgaaacgac ctgtaccttc accaactcgc tagccctggg cacactgacc
     4081 ctggtcaaag tggtcgacaa cctcggtgag agcgggccag gttatcttgg tgtaggtgat
     4141 tttcccttga caatcgatag cgtttccact acttcgggaa ctcccgtgtc agtcactcct
     4201 ggcgatcatg ccatcgcgga aacacctcag agtggttact cggtaggcac ctggacttgt
     4261 gatgatggca caagcgggac tgctggctca tcgacggcaa ctgtcaatgt ttccagcgga
     4321 gagaacgtta cctgcacgat gaccaacacg ctgatcgcga ctccagcctt ggaactggtg
     4381 aagagcgcta ccccgtcaac ctacgactcg gtcggggatg tgatcagcta cagctatgta
     4441 atgaccaaca ccggcaatgt cacgctgatc gcaccattca cggtggacga cgacaaggcg
     4501 actgtaacct gcccggggac acccaccagc ctggctccag gagagggcat cacctgctcg
     4561 gccagctaca ccatctccca gccggacctg gacgccggct cggtgaagaa caccgctcaa
     4621 ggtcacggct actacggcac gaccccggtg gactcgaact tcgatgatga gacggttacc
     4681 gccgttccag acccggtgat aagcatcgtc aagaccggta cctgggtgga cggaaacggg
     4741 gatggctatg cccagccggg tgaggtgatc agctacacct ttgcagtgaa gaacgagggc
     4801 aatgtcacgc tgcacgatgt aacggtcacc gacccgttgc ccggcatgtc agctatctcc
     4861 tgcccgaaga ccacgctggc tgtcggcgaa cccatgacct
//
LOCUS       DBNGSX010000185         8246 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1537908, whole genome shotgun sequence.
ACCESSION   DBNGSX010000185 DBNGSX010000000
VERSION     DBNGSX010000185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8246)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8246)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8246
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1537908"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2426
                     /locus_tag="AC16CB_05785"
     CDS             <1..2426
                     /locus_tag="AC16CB_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012361.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HIR3342013.1"
                     /translation="GGYTHLTELTQGVTISPAQAHRVILWEAVGETEGTGIVHIAPGC
                     GAEDFQLGREYGLPLVAPLDDEGYFVDNFAWLSGLHVADVSQPIFEDLRRKDLLYTVE
                     PYTHRYPTCWRCRSELVFRLVDEWFISMGEVYDKPRQALTADEKARSLRYQIMDVVDQ
                     IRWIPEFGHSREMDWLRNMHDWMISKKRYWGLALPIWVCDSCGHYHVIGDEVELKERA
                     IDGWEKFEGHTPHRPFVDAVKLECPECKGVMSRIPDVGNPWLDAGIVSFSTLRYRTDS
                     DYWRKWYPAHWISESFPGQFRNWFYSLLAMATVIDSSPPFLENFGYATLLAEDGRPMH
                     KSWGNSIEFNEAADNMGVDVMRWLYCAHKPENDLLFGYNRGDDVRRRFIIPLWNVYSF
                     LATYARLDGWEPTLNSTGGESGFDPASPEGPTPHSENLLDRWIVARLNQVTARCTEAL
                     ENSDTYSATLALEALLDDLSNWYVRRSRRRFWKSEHDTDKEDAYTTLYHVLVKLIRIL
                     APFIPFTTEAMYQNLVRAAWETSAVELPKGQSPYTSVHHTAWPQADTATVDESLLDQM
                     ALARQVASLGLSARNAAGLKVRQPLAKVMVYAGGKRTLRDELVEIVTDELNVKSLEFV
                     QDPKRLVSYRILPDNKLLGPRFGARFPALRQAIAEADPYQVASSVSAGLPVSLQVDGE
                     SVELAPEEIVVNTQPAEGLAVAADKLITVAVDATITPELRLEGLAREVVRRVQAMRKD
                     AGFDIADRITTYYAATGEIAQVFDAWGGYIKAETLTTNLVSGDPPEEAFKEVHKVEGE
                     SITLGVKRN"
     gene            2481..3059
                     /locus_tag="AC16CB_05790"
     CDS             2481..3059
                     /locus_tag="AC16CB_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR3342014.1"
                     /translation="MARRKVDFASGQYYHIYNRGANRGLIFHVDDNYLFLLRRLNEHS
                     TKLGISVIAYCLMPNHFHFLLRQDGEFPISHLMQAVFNSYTKAFNKAFGRTGTLFEGP
                     FEATHVDRNDYLVHLCRYIHRNPLDAGLVSNPSQWPFSNYLEWIGKRNGSLVDQAFVH
                     DFFPDPRSYEIFVMEYTPSQEINSAIKRFSFD"
     gene            complement(3103..3858)
                     /locus_tag="AC16CB_05795"
     CDS             complement(3103..3858)
                     /locus_tag="AC16CB_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011229185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HIR3342015.1"
                     /translation="MNGIIIPVVNILAVIGTIVVNGLANALPFNGKGTGEISDQFNVF
                     FVPAGYVFSIWGLIYLGLILFAVYQALPAQRDNPRLSQVGLLFVLSCLANIAWLFLWH
                     YERFPLTLVAMFTLLISLIAIYLRLGIGRTAVSPGERWLVQIPFSVYLGWVSVATIAN
                     VTDVLDYIKWNGWGIGPEVWAIIMLVVGLALTVAMSLTRGDIAYALVVLWAYVGIAVK
                     QAGTPLVAVSAWVAAGLVVVLIVITAVLRPRLG"
     gene            complement(3987..4772)
                     /locus_tag="AC16CB_05800"
     CDS             complement(3987..4772)
                     /locus_tag="AC16CB_05800"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3342016.1"
                     /translation="MHAAPKLVAENVWKTFIPRRGEDVVALQDLSMAVNENEFVALVG
                     PSGCGKSTFLRMVAGLERLSAGAIQVDGHPVTGPGAERGMVFQSYTLFPWLTVFENVR
                     FALKKSGLPRQAQEQRVRDQLTQVGLERFEKAYPNQLSGGMRQRAAIARALVYNPQML
                     LMDEPFGALDAQTRLLMQELLLQVWQTNRTTVLFVTHDVEEAILLSDRIYVMTARPGR
                     LKAEVTVDLPRPRSLMETEALPRFAVLRHQLLALIREEAQKAF"
     gene            complement(4774..5688)
                     /locus_tag="AC16CB_05805"
     CDS             complement(4774..5688)
                     /locus_tag="AC16CB_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3342017.1"
                     /translation="MEDHPQTPDPSSESKPGQPSPDHPAASPSPQRAGGQRGEDKSFR
                     LLELRGDIPRRYYLAAGGAFLGLFFLLWIGVTGLGLVSPIFLPAPGAVFNELLRQLRE
                     GILWQDMSASAYRISTGWLISTALALPIGILMGNFRFFEGLFEPFIDLVRYMPAVAFV
                     PLTILWAGVGDSQKFIILFIGTFFQQVLMVMDNVKNVPFELVQVSYTFGLNKWEVLLH
                     VILRYALPGIWDTLRITLGWAWTYLVVAELVAANVGLGYRIMRAQRFLETDMIILGIL
                     VIGLLGLLTDYAFKITYRWLFPWMLEAN"
     gene            complement(5701..6705)
                     /locus_tag="AC16CB_05810"
     CDS             complement(5701..6705)
                     /locus_tag="AC16CB_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016555514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3342018.1"
                     /translation="MNAKLFRLIILSVLALSLVACGGQSQPTTAAPQEPPAQAEPTTV
                     RIGTQPWIGYGPWWIAKEKGLFEKYGLQAELVDFIEDKEVNAAFASGDMEAANLATHT
                     AIKLFAAGLDMRLVLVEDASYEADAILAPKSLASIADLKGKKIAYEEGTTSDLLLNYA
                     LAQNNMSLEDIEPVFMPASDAGVTLIAGQVDVAVTYEPYITTALTENADLGLLYTAAE
                     RPGLISDVFVVSGKFAAENPDAVRALIKVWDEAMSFYNSNPEEAKAIIAINVGSVPEE
                     LVTAFEGVRFYDLAANQSQLSGDFLKTIQDVAQVAQSIGLVEQIPDLSKLVDASFLQP
                     "
     gene            complement(6767..>8246)
                     /gene="hisD"
                     /locus_tag="AC16CB_05815"
     CDS             complement(6767..>8246)
                     /gene="hisD"
                     /locus_tag="AC16CB_05815"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR3342019.1"
                     /translation="ELPPGYPGGFIILLYPLYPSVEGKENTCSANVEENGKEMRDVLR
                     VFNAETARKGLLKRKPPNEFTVPPSVMERIAMLFGDGFTPAQAVERILQDVRRRGDAA
                     LFEWTEKLDGVALHDLRVPQFELQAALEALPEGLRLSLEKAAQRIEAFHKRQPLTSWK
                     TQEDGGWLGQLIRPIRRVGLYVPGGSAPLPSSVLMSAIPARVAGVPEIVLVTPPVWGT
                     TRVDPTILAAAALTGVQEVYMLGGAQAIAALAYGTQNIPAVDKVFGPGNLFVTLAKQQ
                     VFGVVGVDGLAGPTETVVVADENANPAWVAADLLAQAEHDVLASAILLTPSLQLAEAV
                     QVEVARQLEGYADYVPLARGEIIAAALENRGGIILTRDLDEAIELANFYAPEHLALSV
                     VDPWRWTEKIQAAGGVFLGEHSFEVLGDYVAGPSHVMPTGGSARYASPLNIWDFVHIV
                     SLVALDPAIANQIGSAAAQIATAEGLDAHARAALLRLNTQEH"
BASE COUNT         1907 a         2398 c         2233 g         1708 t
ORIGIN      
        1 ctggcggcta tacccacctg accgagctca cccagggtgt gacaatttcg cctgctcagg
       61 cgcatcgggt catcctatgg gaagcggttg gcgagacgga gggtactggt atcgttcaca
      121 tcgccccggg ctgcggtgcc gaggacttcc agctagggcg agaatatggc ctgccactgg
      181 ttgccccgct tgacgatgaa ggctacttcg tagacaattt cgcctggctg agcgggctgc
      241 atgtagcgga tgtatcgcag cctatcttcg aagatttgcg ccgcaaagat ctgctataca
      301 ctgttgagcc ctatacccac cgctacccca cctgctggcg ctgccgttcc gagctggttt
      361 tccgcctggt agatgagtgg ttcatcagca tgggcgaggt atacgacaag ccgcgccagg
      421 ccttaactgc cgacgagaaa gcgcgcagcc tgcgctacca gattatggac gtggtcgacc
      481 agatccgctg gatacctgaa ttcggccact cgcgcgaaat ggattggctg cgcaacatgc
      541 acgactggat gatcagcaag aaacgctatt gggggctggc gttgcccatc tgggtgtgtg
      601 atagctgcgg gcactaccat gtcattggcg atgaggtgga gctgaaagag cgcgccatcg
      661 atggctggga gaagtttgaa ggccatacgc cacaccgtcc tttcgtggac gcggtcaagc
      721 tggaatgccc tgaatgcaaa ggggtaatga gccgcatccc cgacgtgggc aacccctggc
      781 tggacgccgg catcgtatcc ttcagcacgc tgcgctaccg cacagattca gattactggc
      841 gcaagtggta tccggcgcac tggattagcg aatccttccc aggccagttc cgcaactggt
      901 tctacagcct gctggctatg gcaacggtca tcgacagcag cccacccttc ctggagaact
      961 tcggttatgc caccctgctg gccgaggatg ggcgcccgat gcacaaatct tggggcaact
     1021 ccatcgagtt taacgaggct gccgacaata tgggcgtgga tgtgatgcgc tggctgtact
     1081 gtgctcacaa gccggagaat gacctgcttt tcggctataa ccgcggtgac gacgtgcgcc
     1141 gccgtttcat catcccgttg tggaacgtgt acagcttcct ggccacctat gcccgcctgg
     1201 acggctggga gccaaccctc aactcaacgg ggggtgagag cggcttcgac cccgcctcgc
     1261 cggaaggtcc cactccacac tccgaaaacc tgctggaccg ctggattgtg gcacggctga
     1321 accaggtgac tgcccgctgc accgaagctt tggagaattc agacacatac agtgccaccc
     1381 tggctctgga agccttgcta gacgatttat ccaactggta cgtgcgccgc agccggcgcc
     1441 gcttctggaa aagtgagcac gatacagaca aagaagatgc ctacactacc ttgtaccacg
     1501 tgctggtcaa gctgatccgc atcctggcgc cctttatccc gttcacgacc gaagccatgt
     1561 accagaacct ggtgcgcgct gcatgggaaa cctcagcagt tgaactgccg aagggtcaat
     1621 ccccttacac aagcgtgcac cacaccgcct ggccccaggc agacacagct accgtggatg
     1681 aatccctgct cgaccagatg gccctggcgc gccaggtagc cagcctgggt ctgagcgctc
     1741 gtaacgcggc cgggttgaag gtgcgccagc cgctcgccaa agtgatggtc tacgccggcg
     1801 gaaagcgaac attgcgcgac gaactggtgg agatcgtcac cgatgagctg aatgtcaagt
     1861 cgttggagtt tgtccaggac ccaaagcggc tggtgtcgta ccgcatcctg ccggataaca
     1921 aactgctagg accccggttt ggggcgcgct tccccgcgct gcgccaggct atagctgagg
     1981 ctgaccccta ccaggtggca agcagtgtgt ccgcggggct gccggtcagc ctgcaggtgg
     2041 atggagagtc ggttgagcta gccccggagg agatcgtggt gaacacccag ccagccgaag
     2101 gtctggccgt agcagctgat aagctgatca ctgtggctgt agatgctacc atcacgcccg
     2161 agttgcgcct ggagggtctg gcgcgcgaag tggtgcggcg cgttcaggcc atgcgcaaag
     2221 acgctggctt tgacatcgcc gaccgtatca ccacctatta cgcggcgaca ggcgagatag
     2281 ctcaggtctt cgacgcctgg ggaggctata tcaaggctga gacgctgacc acgaacctgg
     2341 tttcaggcga cccaccggag gaggctttta aagaagttca caaggtggaa ggggagtcaa
     2401 tcacccttgg tgtcaaacgg aactgagtaa cgtgcgacgc acttcgaaag tgcgtcgcac
     2461 gttggtcttc aagaattaac atggcgcgca gaaaagtcga ctttgcgtct ggtcagtatt
     2521 accatatcta caaccgtggg gcaaatcgag gattgatctt tcacgtagac gacaattact
     2581 tattcttgct tcgccgccta aatgagcatt caacaaagct tggaatcagt gtcatcgcct
     2641 attgcctgat gccaaaccat tttcactttc tgctacgcca agatggcgag tttccgatta
     2701 gccatcttat gcaagcggtt ttcaatagct ataccaaagc attcaacaaa gcatttggga
     2761 gaactggtac actatttgaa gggccatttg aggcgaccca tgtagatcga aatgattatc
     2821 tcgttcatct atgtcgttat atccatcgta acccacttga tgctgggtta gtctctaatc
     2881 caagtcagtg gccgttctcg aattatctag aatggattgg taagcgtaat ggctcgctgg
     2941 tagatcaggc tttcgttcat gatttctttc ctgatccaag aagctatgaa atattcgtta
     3001 tggaatacac cccatctcag gaaatcaaca gtgcaattaa gcggttcagc tttgattagg
     3061 tgcgacgcac tttagaagtg cgtcgcacct ggatcgcagg cattatccca gcctgggacg
     3121 cagcacggca gtgatcacaa tcaacaccac caccaatcct gctgctaccc aggcggagac
     3181 ggctaccagc ggtgtgccag cctgctttac ggcaatcccc acataagccc acaggaccac
     3241 gagcgcatag gcgatatcac cccgggtgag gctcatggcg acagtgagcg ccagaccaac
     3301 caccagcatg atgatcgccc acacctccgg gccgataccc cagccgttcc actttatata
     3361 atccagcaca tcggtcacgt tggcgatggt cgccacactc acccagccca ggtacacgct
     3421 gaacgggatc tggacgagcc agcgctcgcc tggggaaacg gctgttcgcc cgatccccag
     3481 ccgcaggtag atggcaatca gcgagatcag gagcgtgaac atagccacca gggtcagagg
     3541 gaagcgctcg taatgccata agaacagcca ggcgatgttc gccaggcaac ttaagacgaa
     3601 gagcaaacca acctggctca gccgcgggtt atccctctgc gcggggagcg cctggtagac
     3661 ggcaaacagg atcaacccta aatagatcag cccccagatc gagaagacat agcccgccgg
     3721 gacaaaaaac acattaaact ggtccgaaat ttcgcctgtg cctttgccat tgaatggcag
     3781 cgcgtttgcc agcccattga caacgatagt tcctataaca gctaagatat taaccactgg
     3841 aatgatgatg ccgttcattg ctccctccgt tatgttgcgc ttatctgtcc cagctgagca
     3901 ttccgcgcag cttgcttacc cgcaatcatt ataaggaaac cctcgctgca agtcaatcac
     3961 tcctttaaaa atggatcttt cgcaggttaa aaggccttct gtgcctcttc gcgtatcaat
     4021 gccagcaact ggtgacgcaa aacagcaaat cgcggcaagg cctcggtctc catcagcgag
     4081 cgcggtcgag gcagatctac tgtgacctct gctttcagtc gcccgggacg cgctgtcatc
     4141 acatagatgc gatctgagag caaaatggcc tcctccacat cgtgggtcac aaaaagcacc
     4201 gtggtgcggt tggtctgcca gacctgtaat agcagctctt gcatcaataa gcgcgtctga
     4261 gcatccagcg cgccaaaagg ctcgtccatc aagagcatct gcgggttata taccagggcg
     4321 cgtgcgatgg cggcccgctg ccgcatcccg cccgagagct gatttggata ggccttctca
     4381 aagcgctcca gccccacttg agtgagctgg tctcgtacac gctgttcctg cgcttggcgc
     4441 ggcaagccgc tcttcttcaa ggcaaagcgc acgttttcaa agacagtcag ccaaggaaaa
     4501 agggtatagc tctggaagac catgccgcgt tctgcacccg ggccagtcac aggatgaccg
     4561 tctacctgga ttgcccctgc gctcaatcgt tccagtccgg cgaccatgcg cagaaaggtg
     4621 gacttgccgc atcccgaagg ccctactagg gccacaaatt cgttctcgtt aaccgccatg
     4681 ctcaaatcct gcagtgccac aacatcctcc ccgcggcgcg ggatgaaggt cttccacacg
     4741 ttttcggcga caagcttggg ggcggcatgc atcttagttt gcctccaaca tccagggaaa
     4801 cagccatcga taagtgatct taaaggcata gtctgtcagc agacccaaca agccgatcac
     4861 caaaataccc aggatgatca tgtccgtttc caagaaacgt tgggcgcgca tgatgcgata
     4921 gccaagtccc acgttggcag ctaccagctc cgccacaacc aggtatgtcc atgcccaacc
     4981 cagggtgatt cgtagcgtat cccagatccc cgggagggca taacgcagga taacatgcaa
     5041 caacacttcc cacttgttca agccaaaagt gtaactcacc tggaccagtt cgaagggcac
     5101 gttcttgaca ttatccatca ccatcagaac ctgctgaaag aaagtgccga tgaacaggat
     5161 gatgaacttc tgcgagtcgc ccacgcctgc ccacaagata gtcagcggca cgaaggctac
     5221 cgcaggcatg tagcgcacca ggtcaatgaa cggctcgaaa agaccttcaa agaaacgaaa
     5281 gttgcccatc agtatgccta tgggcaatgc cagagctgtg gagatcagcc agccagtgga
     5341 gatgcgatac gcactggcag acatatcttg ccaaaggatg ccttctcgta attgccgcag
     5401 taactcatta aacacagccc ctggcgcagg aaggaagatg ggcgaaacca gacccaggcc
     5461 ggtcacacca atccacagca agaagaataa tcccaggaaa gcccccccag ctgctagata
     5521 gtagcggcgt ggaatatccc cgcgtagctc aaggaggcga aaggacttgt cctcaccccg
     5581 ctgtccccct gccctttgtg gggaggggga ggctgcggga tggtctgggc taggctgccc
     5641 tggtttactc tcagaggaag gatctggagt ttgaggatga tcttccattc gatttttggc
     5701 ttatggctgc agaaaagaag catccactaa cttcgacagg tcaggaatct gctcaaccag
     5761 acctatcgac tgtgctacct gggcaacatc ctgaatggtc ttgaggaagt caccgctgag
     5821 ctggctttgg ttagctgcca gatcataaaa gcgcacgcct tcgaaagcag tgaccaattc
     5881 ttccggaacg ctacccacgt tgatggctat gattgccttg gcctcttcgg gattcgagtt
     5941 gtaaaagctc atggcctcgt cccatacctt aatcaaggcg cgcaccgcat ctggattttc
     6001 cgcggcgaac ttgccgctga ccacaaagac atccgagatc aggccggggc gctcggcggc
     6061 tgtgtagagc agaccgaggt cagcattttc agtcagtgcg gtggtgatgt aaggttcgta
     6121 ggtcacagcc acatccacct gtccggcgat caaggtaaca ccggcatcgg aagcaggcat
     6181 aaagaccggc tcgatatcct ccaggctcat gttgttctgc gccagagcgt aattgagtag
     6241 caggtcactg gtcgtgcctt cttcgtaggc gatcttttta cctttcaggt cggcgatgga
     6301 ggccagcgac ttgggggcaa ggatggcatc ggcctcgtag gatgcatctt ccacgagaac
     6361 caagcgcata tccagcccag ccgcgaagag ttttatcgca gtgtgggtcg ccaagttggc
     6421 ggcttccata tctccagacg caaatgcagc gttgacctct ttatcttcga tgaagtccac
     6481 cagttcagct tgcagcccgt acttctcaaa caggcctttc tccttggcga tccaccaggg
     6541 gccataacca atccaaggct gcgtgccaat ccgcacagtt gtgggttcag cctgggcagg
     6601 tggctcctgt ggtgctgctg tggtgggttg cgattgccca ccgcaggcga ccaggctcag
     6661 agccaggacg gataaaatga tcagacgaaa tagtttggcg ttcatgttct tctcctaaaa
     6721 aatgatttat ataaacacag tgagcacgca ggtgcagaaa agatacttag tgctcttgtg
     6781 tgtttaatcg caataaagct gcccgagcat gggcatccag cccttcggct gtggcaatct
     6841 gggcggcggc tgacccaatc tgattggcga tagctgggtc gagcgcaacc aggctgacaa
     6901 tgtgcacaaa atcccatatg tttagcggcg aggcatagcg cgccgatccg cctgtgggca
     6961 tcacgtggct cggcccggcg acgtagtctc ccaatacctc gaaggaatgc tctcccaaga
     7021 agacgccccc agcagcctga atcttttccg tccaacgcca cggatcaacc acggacagcg
     7081 ccaagtgttc cggcgcgtaa aagttagcca gctcaatcgc ttcgtccagg tcgcgagtca
     7141 atataatccc tccgcggttc tccagcgccg cagcgattat ctcacctcgg gccagcggga
     7201 cgtaatcagc gtacccttcg agctggcggg cgacttccac ctggaccgct tcagccagct
     7261 gcagagatgg tgttaacaag atagccgagg ccagtacgtc atgctccgcc tgtgccagca
     7321 ggtcagcggc tacccaagcc gggttggcat tctcatcggc taccaccact gtttcggttg
     7381 gtcctgccaa gccatctacc cccaccactc caaatacctg ctgcttggct agcgtgacga
     7441 acaggttgcc tggaccgaaa accttatcaa ccgctgggat gttttgcgtt ccataggcca
     7501 aggcagcgat agcctgcgca ccgcccagca tatatacctc ttgcacgccc gttaaagccg
     7561 ccgccgccaa aatggtgggg tcaacacgtg tcgttcccca aacgggaggg gtgaccagta
     7621 cgatctcagg aactcctgcc acccgcgcag ggatggctga catgagcacc gaggatggca
     7681 aaggggcgct tccaccgggc acatacaaac ccacacgtcg gattggccgg ataagctgtc
     7741 caagccaacc accgtcttct tgggttttcc aagaagtgag cggctgtcgc ttgtggaaag
     7801 cctcaatgcg ctgggcagcc ttttcaaggg atagccgcag cccttcaggc aaggcctcca
     7861 acgcggcctg caactcgaac tgcggcacgc gcagatcatg tagagccacg ccatccagct
     7921 tttctgtcca ttcaaacaaa gcagcatcac ccctcctgcg aacatcctgg aggatgcgct
     7981 ccaccgcttg ggcaggagtg aacccatctc caaacagcat cgcgatgcgc tccataacgc
     8041 tgggcggcac agtgaattca tttggtggtt tacgcttgag cagccctttt cgggctgtct
     8101 cagcattgaa tacgcgaagc acgtctctca tctccttgcc gttctcctca acatttgcag
     8161 aacaagtatt ttcttttccc tctacgcttg gatagagggg atagagcaaa ataataaaac
     8221 cccccggata accggggggt aattca
//
LOCUS       DBNGSX010000186        11898 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_845863, whole genome shotgun sequence.
ACCESSION   DBNGSX010000186 DBNGSX010000000
VERSION     DBNGSX010000186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11898)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11898)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11898
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_845863"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..424)
                     /locus_tag="AC16CB_05820"
     CDS             complement(221..424)
                     /locus_tag="AC16CB_05820"
                     /inference="COORDINATES: protein motif:HMM:NF053320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF8156 family protein"
                     /protein_id="HIR3342020.1"
                     /translation="MGEGICLRSSDQLALDELYAAAHNHLAEAAYAAHPLPMQIFLLA
                     MLLEEHKEVMSLRNLIEEMLAVG"
     gene            complement(440..513)
                     /locus_tag="AC16CB_05825"
     tRNA            complement(440..513)
                     /locus_tag="AC16CB_05825"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(477..479),aa:Val,seq:tac)
     regulatory      586..753
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            862..1821
                     /locus_tag="AC16CB_05830"
     CDS             862..1821
                     /locus_tag="AC16CB_05830"
                     /inference="COORDINATES: protein motif:HMM:NF013648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3342021.1"
                     /translation="MHAIRNRRRVVALLCMGFILSACASATPAPTQLPAATPTPTGIS
                     IVDGMGREITLDRPAQKVASLAPSNTEILFAIGAGAQVIARDSFSDFPEQALAVADIG
                     GGFGEIDTETLVSLEPDLVIAAEINPVENVQMLEDLGLKVFYLSNPKELDGLYENLRT
                     VARLVGHEAETEGLIESLKLRVSTIEEKVSSLQERPLVFYELDGTDPSAPWTSGPGTF
                     IDLLLTKAGGDNLGNTLDSEWVQISVEELITQNPDVILLGDYTWGGITPEDIAARPGW
                     EVIAAVQNKQVYTFDDNLVSRPGPRMVDGLEALAELLHPELFQ"
     gene            1822..2850
                     /locus_tag="AC16CB_05835"
     CDS             1822..2850
                     /locus_tag="AC16CB_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013615609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR3342022.1"
                     /translation="MNISPRPILINLALLVIAALLSIGVGPVFIPPDTVTRMLVDRLP
                     WIAINPDWPEAFSAILFQIRLPHTALMLLTGAALGGSGAAYQGLFRNPLADPYLIGVA
                     SGAALGAVAALTIQWPSSLMGMYTLPASAFLGAMITITVVYNLARVGRAVPTTTLILA
                     GVAFSAFATSLMSFLMLRSNTEMRRALTWLLGGAIMSGWQPVMAMLPYELIGLGILAA
                     SGHALNVMQFGEEQAQQLGLPIERIKLFLIVAASITTAAAVSFSGVIGFVGLVAPHIV
                     RLLWGPDYRRLTPLAILAGGSALLLADMLARIVLAPQILPVGIVTALAGAPFFLWILR
                     RAKTQAYW"
     gene            2863..3672
                     /locus_tag="AC16CB_05840"
     CDS             2863..3672
                     /locus_tag="AC16CB_05840"
                     /inference="COORDINATES: protein motif:HMM:NF010068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HIR3342023.1"
                     /translation="MLNIHSLSVAYQERIVLDDLSLEVARGEILAVVGPNGVGKSTLV
                     RALSGVLPPVSGQVNLENRDLTQLSPMQRARLMAVVPQARELPGAYTVYQTVLLGRTP
                     YLSWLGHAGTGDHAAVRVALEQTRTLDLAERPVGELSGGEQQLVLLARALAQDAPVLL
                     LDEPTTHLDLAHQSNLLNLVRRLSKEKNLTVLMVLHDLNLASLYADRVALMVSGRIHA
                     IGKPSEVLTTDNLSAVYQVAVNVIPHPDYGNPLILPDGLNSLEAGETVVTH"
     gene            3683..4717
                     /gene="cobT"
                     /locus_tag="AC16CB_05845"
     CDS             3683..4717
                     /gene="cobT"
                     /locus_tag="AC16CB_05845"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HIR3342024.1"
                     /translation="MIQPLDKGAMQSARSRQDRLTKPQGSLGQLEALSVHIAGITGQS
                     QPNLDHKVVAVMAGDHGVARQGVSAYPPDVTQQMVLNFLQGGAAINVLARHTGARVVV
                     VDMGVAADLPPHPDLKIKKIAYGTENIATGAAMSRSQAEQAIRNGADVILEEIALGLD
                     ILATGDMGIGNTTPSAAIAVSITGLPVESIVGRGTGIDEAGLKRKVAAVRSALEVNQP
                     NPHDGLDVLAKVGGFEIAGLVGAILAAAAHRRPVVIDGFISTAAAMIAVSLCPLAGGY
                     LIASHASQELGHRHMLESLQLKPLLDLELRLGEGTGAVLAMSIVEAACKILSEMATFD
                     EAGVSEKSAG"
     gene            4721..5497
                     /gene="cobS"
                     /locus_tag="AC16CB_05850"
     CDS             4721..5497
                     /gene="cobS"
                     /locus_tag="AC16CB_05850"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HIR3342025.1"
                     /translation="MQDEGTQDTQGRAPLPGVGFLAAIQFLTIAPPIIRRPFSAREIG
                     GAVAYFPLVGAILGALLAISDALLAGIFPSPVRSALLLSLWVLLTGALHLDGFLDSLD
                     GLLGGGTPENRLAIMRDERVGAYALAGGILLLLLKFSALLSMPERWAALVIAPTLSRW
                     GMSLAIVAFPYARSEGLGRVMKDHATWLHASAAALTALAIAWFYGQRSGLILLAVSAL
                     TVILVARFVLKRIPGLTGDIYGALNELLEVLTLLTWEILH"
     gene            5479..6039
                     /gene="cobU"
                     /locus_tag="AC16CB_05855"
     CDS             5479..6039
                     /gene="cobU"
                     /locus_tag="AC16CB_05855"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HIR3342026.1"
                     /translation="MGNLTLILGGARSGKSTFALNLAEKIRGCVAFIATAQPGDPEMA
                     ARIAAHQRQRPADWRTLEIPHAIAATWLGQEIDADLIILDCLTLLVSNLAVMASPDVD
                     HPNEGKFTELVCSEINTLIDLIQNSPGDWIVISNEVGLGLVPPYPLGRIYRDWLGWAN
                     QRLATVASEVYLLVAGIPVPIHNYRL"
     gene            complement(6073..6846)
                     /gene="pgl"
                     /locus_tag="AC16CB_05860"
     CDS             complement(6073..6846)
                     /gene="pgl"
                     /locus_tag="AC16CB_05860"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HIR3342027.1"
                     /translation="MSGKSSASAINSKTHVFQNIEVLAGGAAGYILSAMQEALAVQDR
                     FAMMLSGGSTPRAVYTLLASSPYKQAVDWSQVHIFWGDERCVPPDHPYSNYRMAGEVL
                     ISKVNLPAENVHRIRGEIPPDQAAASYEIAISSYFGSQTAFPHFDLILLGMGEDGHIA
                     SLFPGSPAINETARWVVAVDHDQPPPPLVSRVTVTLPVINAARRVALIVAGEAKASTV
                     AATLTTPEKSLVLPVQRVNPVEGELVWLLDEAAAGKITN"
     gene            complement(6815..7453)
                     /locus_tag="AC16CB_05865"
     CDS             complement(6815..7453)
                     /locus_tag="AC16CB_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR3342028.1"
                     /translation="MLKSVFGRRDDEERIKQEGRLPPGQSLTNRFPVLHYGPTPRFNP
                     ATWDLRVFGEVEEEVVWNWDEFNKLPRTKISMDIHCVTRWSKFDTEWEGVSIKTLIDQ
                     GYIKLKPTARHLMQHCEYGFTVNIPLEVALAPNFLLATHFNGEPLEPDHGYPLRGVVG
                     NIPGTDFTTPYFWKGGKWIRGLEFMAQDRLGFWEQAGYHNEADVWKEQRFGY"
     gene            7856..8692
                     /locus_tag="AC16CB_05870"
     CDS             7856..8692
                     /locus_tag="AC16CB_05870"
                     /inference="COORDINATES: protein motif:HMM:NF013539.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR3342029.1"
                     /translation="MLYEDRIRRERAEMSKSFAKLADFLLNSYIEAAFMTASELAHTL
                     DLDAATVVRFSQFLGYSGFPELQREIRSRVKKDLLIRPKQAEDDNSTPGIVSRAMQEL
                     NLALEQTRITLDTDAVERLVEQIGNVRRTVVLAEGPAQPTAYSLVHYFEQGGFPIYIA
                     RPGIADLARTVNTATSQDLFLAIEVAGQSPYIARALGEAKAKNIPTAAIVGASSLASA
                     RAADIVLAAHAHPSLGVGILTIEAIVYAFAQVLRLRYADNFVGTEQAIAELTARIQTN
                     TD"
     gene            8697..9467
                     /locus_tag="AC16CB_05875"
     CDS             8697..9467
                     /locus_tag="AC16CB_05875"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR3342030.1"
                     /translation="MRIIRYQTKNDPPQYGWVLENAVGAIEGDPFGEFRRLEANQNLD
                     SVRLLAPVLPGKIICVGRNYAAHAREHDAEVPEVPLLFLKAPSAVIGPGDKIVLPPQS
                     QQVEHEAELAVVIGRRGRWISPEEALEHVMGYTIGNDVTARDLQFRDNQWTRAKGFDT
                     FCPLGPWIQTDFDPTDTLITCHVNGEMRQMASTRDMVFSVRQLIAFTSSIMTLEPGDI
                     IMTGTPSGVSPLQPGDTVKVSIEGLGELENSVVSQPPR"
     gene            9467..10495
                     /locus_tag="AC16CB_05880"
     CDS             9467..10495
                     /locus_tag="AC16CB_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342031.1"
                     /translation="MPEGIRLPISLDIHTAVQTALVIVGLLIVLFFWLGIRSIRTARR
                     LRFFRMRRDRMVRGWRLIITAIILIPIAILLNRRLEPLIYTYYQPTVTVTQTPTITIT
                     PTISQTPTITLTPTITLTPLVSNTPTITPTPFIPPAIEARFESTITPNPESVFSPLTF
                     TDGLDALYRPLKEGTVFENPVEHMYAVFSYDGMIRGSQWTALWYRNGELVHFETLPWD
                     GETGGLGYTDWQPQPEEWFTGTYEVQIFVGRDWKVSGTFTVEGEPPTPVPTATPTTTP
                     TSTYTPTSTKTRLPTRTYTPIPPTRPPYISPTPTKTPTPWPTLTRTPITPSITPQPTR
                     TRTVTPSP"
     gene            complement(10569..11552)
                     /locus_tag="AC16CB_05885"
     CDS             complement(10569..11552)
                     /locus_tag="AC16CB_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003334710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HIR3342032.1"
                     /translation="MTNWLSSSSKIKKLPPYFSLTQEVTHALELNLPLVALETTVITH
                     GLPYPDNLTLADNMEDEVRNQGVTPATIGVIDGRVQVGMVPAQLSFLATSGGELLKIS
                     LRDFAPAIASLASGGTTVAGTMYAAHAVGIHVFATGGIGGVHFELSSRRKGAFDVSAD
                     LPALANIPMIVVCAGAKAILDLQATLEYLETWGVPVVGYQTDDFPAFYSSRSGLKTST
                     RADTPEEVAFVARSHWALGMKSAILVTVPPPDEVALPSEAVKKAVRAALKAAQTEKIT
                     GKEVTPFLLSRVSELTEGSSLRANLGLLLNNANVASQIARALSRMEREGIV"
     gene            complement(11533..>11898)
                     /locus_tag="AC16CB_05890"
     CDS             complement(11533..>11898)
                     /locus_tag="AC16CB_05890"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR3342033.1"
                     /translation="AEAAVLSERTSPVGGRRQALEVAKYLISQGVDIVIITMAEQGLC
                     YATSESSGYIPAIKTSILDPTGAGDALTATVIFALLNDIPLDDAVRLGVSAASLTLRF
                     RGAVVPDLSLEKLYDQLVI"
BASE COUNT         2742 a         3375 c         3074 g         2707 t
ORIGIN      
        1 ttacttagga atcaacagcg aattttccag tgtctgtccc aaaattggtg gggaaaatca
       61 aagagaacag gaagacaggc atagcccgat gttactcaat ctctcaaccc aaccccgatc
      121 cagctgccat caatccagcg agcaaccccg ccttagtttt gtagccccgc accgtgtggc
      181 tgcgcgtctc catgcgccca aagatctgtt cccttgcaga ttatcctact gccaacattt
      241 cttcaattaa gttgcgcaag ctcatcactt ccttgtgctc ctccaacaac atagccaaaa
      301 ggaatatctg cattggcaat ggatgtgcag cataggcagc ctcagctagg tgattgtgtg
      361 ctgccgcgta cagttcatca agggcaagct ggtcacttga ccttaaacaa atcccctctc
      421 ccaaattggg agagggctgt gggcggaaca ggactcgaac ctgcgacctt ctctgtgtaa
      481 gagagacgct ctaaccaact gagctatccg cccgcgtgtg gcgattatac caaaatcccc
      541 gctggttaac gggaattttg gtataatcct tcgtagcaaa attaacaatt ttataccgat
      601 tgaggtgccc aacccctcgg aagggagggc ttaaaggcga agccggtgac gtcagaaaga
      661 tgaatttaga cgaagtccgg cgctgtcccg caactgtagg ttagtccatt agggctaaca
      721 agccaggtcg cccgcctcac tcgccgtttc cacaaacctc gcggaaagga ggtgggactt
      781 ttcaccaaaa gaccccaacc ccctgccaag aaggcagggg attttgctta atcaacttag
      841 ttttataaac gaggagaatc gatgcatgcc ataagaaacc gccggcgggt tgtcgccctg
      901 ctgtgtatgg gctttatcct gagcgcctgc gcttcagcca cacccgcccc cacgcagctt
      961 cctgctgcaa cccccacacc aaccggcata tccattgttg acgggatggg tagagagatt
     1021 accctggata gaccagccca gaaagtggcc tcattggccc cctccaatac agagatcctt
     1081 tttgctatcg gtgcaggcgc acaggtgatc gctcgcgata gcttttccga ttttcctgag
     1141 caagccttag cggttgcgga tatcggtggc gggtttggag agatcgatac agagactctg
     1201 gtatcgctcg agcccgacct ggtgatagca gccgagatca acccggttga gaacgtgcaa
     1261 atgctggaag atcttggtct caaagtattc tatctctcga atccgaaaga attagatggg
     1321 ttgtacgaga atcttcgcac tgtagcccgc ctggtagggc acgaggctga aaccgaaggg
     1381 ctcattgaga gcctaaaact gcgcgtttct accatcgagg agaaggtaag tagccttcaa
     1441 gagcgccccc tggtcttcta cgagctggat gggaccgatc ccagcgcccc ctggacatct
     1501 ggcccgggga cattcatcga cctgttgttg accaaagccg gtggtgacaa cctgggcaat
     1561 accctggaca gcgaatgggt ccaaatcagc gtggaggagc taatcaccca aaatcctgac
     1621 gttatcctgc tcggcgatta cacctggggt ggcatcacac ctgaagatat cgccgctcgc
     1681 cccggatggg aggtaatcgc tgccgtgcag aataagcaag tgtatacctt tgatgacaac
     1741 ctggtcagcc gtccaggccc gcgcatggta gatggattgg aagctctggc agagctgctg
     1801 cacccggaac tgttccaata gatgaacatc tctccacggc ccattttgat taacttggcc
     1861 ctgctcgtca tcgctgcgct gctgagcatc ggcgtagggc cggttttcat cccacctgat
     1921 acagtgaccc gcatgctggt ggaccggttg ccgtggatag ccatcaaccc ggattggccc
     1981 gaagccttct cggctatcct tttccaaatt cgcctgccac acacagcttt gatgctactc
     2041 acaggcgcgg cgctgggggg cagtggcgcc gcctaccagg gtctgttccg caatccatta
     2101 gcagatccat atctgatcgg tgtggcgtct ggagcagcat tgggcgctgt agccgctcta
     2161 accatccaat ggcccagctc actcatgggc atgtacacat tgccagccag cgcctttctc
     2221 ggtgctatga tcacgatcac tgtggtatat aacctggctc gcgtggggcg agcggtgccc
     2281 accacaacgc tcattctagc gggagtggca ttcagtgcct tcgccacctc gttgatgtca
     2341 tttctcatgc tgcgctccaa cacagagatg cgccgtgccc ttacatggct gctggggggc
     2401 gccatcatga gcgggtggca gcctgtcatg gctatgttgc cctacgagtt gatcggtttg
     2461 ggcatcctgg cagcttctgg acacgctcta aatgtgatgc agtttggtga ggagcaagca
     2521 cagcagttag gcctgcccat cgagcgcatc aagttgttcc tcatcgtagc cgcctctatc
     2581 acgaccgccg cggcggtttc cttttcgggg gtcatcggct tcgtcggctt ggtagcgccg
     2641 catatcgtcc gcctgttatg gggacctgat taccgcaggc tcacacccct ggcgatcctt
     2701 gcagggggca gcgccctctt gctagccgat atgctggcgc gcatcgtctt ggcaccccaa
     2761 attttgcccg tgggcatcgt cacagccctg gctggcgcac cctttttctt gtggatcttg
     2821 cgccgggcga agacacaagc ctattggtaa acaggaggtc ccatgttaaa tatccattcc
     2881 ttatccgtag cgtatcagga acgcattgtg ctggacgacc tcagcttgga agtagcccgt
     2941 ggtgagatct tagctgtggt tggacccaac ggggtaggca aatccaccct ggtgcgcgcc
     3001 ttgagcggtg tgctcccgcc tgtgagtggt caggtgaatt tagaaaatag agacctcacc
     3061 caactttcgc ctatgcagcg tgcacgcctg atggctgtcg tgccccaggc gcgcgagttg
     3121 cccggtgcct acacggtcta ccaaaccgtg ctgctagggc gcacgccgta tttgagctgg
     3181 ttaggacatg ctgggacagg cgatcacgct gctgtgcggg tcgctttaga gcaaacccgc
     3241 acgctggatc tagctgaacg acctgtggga gagctctctg ggggtgagca acagctggtg
     3301 cttctggcgc gagccctggc gcaggatgca cccgtgctgc tgcttgatga acccaccact
     3361 cacttagacc tggcccacca gtccaatttg ctcaacctgg tgcgccgctt atccaaagaa
     3421 aagaatttaa ccgtcctgat ggtattgcac gatctgaacc tggcaagcct gtatgcagat
     3481 cgggtggcgc ttatggtctc aggtcgcatc cacgccatag gcaagccctc cgaagtgcta
     3541 accactgaca acctgtctgc tgtttaccag gttgcggtca acgtgatccc tcatcctgat
     3601 tatggcaacc cgctaattct gccggatgga ttaaactcct tagaagccgg agaaaccgtt
     3661 gtcactcact gaaattctgg caatgatcca gcctctggat aagggcgcta tgcaatctgc
     3721 gcgctcccgc caggataggt taacgaaacc acagggcagt ctggggcaac tggaggcatt
     3781 atctgtacat attgcgggca ttacgggtca gtcccaaccc aatctcgacc ataaagtcgt
     3841 tgccgtgatg gctggcgacc acggggtggc tcgccaggga gtaagcgctt accctccgga
     3901 cgtaacacag cagatggtgc tgaatttctt gcaaggcggc gcagccatca acgtacttgc
     3961 tcgacatact ggggcgcgtg tggtggtggt tgatatgggc gtagccgctg atttgccgcc
     4021 gcatccagac ctaaaaatca agaagattgc ttatggaacc gaaaacatcg ccactggagc
     4081 ggcaatgagc cgctcacagg ctgagcaggc tatccgaaat ggggcagatg tgatactcga
     4141 agagatcgct ctcgggctgg acatcctggc aacgggtgac atgggtattg gaaataccac
     4201 cccctccgcc gccatcgccg tgtcaataac cggactaccc gtcgagtcta tcgttggtcg
     4261 cggcactggt atcgacgaag ccgggctgaa acgaaaggtc gccgcagtac ggagcgctct
     4321 ggaagttaat cagcccaatc cccatgatgg attggatgtt ctggctaaag ttggcggatt
     4381 tgagattgcc gggttagtgg gcgcaatcct ggcagccgca gcacatcgcc gtccagtagt
     4441 gatcgatggg tttatctcca ctgcggcggc catgatcgcc gtcagccttt gcccgctggc
     4501 aggcggttac ttgatcgcct ctcacgcgtc gcaggaattg ggtcacaggc acatgctgga
     4561 atcgttgcag cttaaaccgc tgcttgacct ggagctgcgc ttgggtgaag gaaccggagc
     4621 cgttttagcg atgtcgattg tcgaagccgc ctgtaaaatc ctgagcgaaa tggccacatt
     4681 cgatgaagcc ggcgtatccg aaaaaagtgc agggtgatag atgcaagatg aaggcacaca
     4741 ggatacgcaa gggcgcgcac ctttgcctgg ggtgggattc ctggcagcta tccagtttct
     4801 caccatcgct cccccgatca ttcggcgccc cttttctgcc agggaaatcg gcggggctgt
     4861 agcttatttc ccgctggtag gtgcaatact tggagcactc ctggcaatat ctgacgccct
     4921 cctggcgggg atcttcccgt ctcctgtgcg gagcgcctta ttgctctctc tatgggtact
     4981 gctaactgga gccctgcatc tggatggctt cctggacagc ctggatggcc tcctcggagg
     5041 cggcacgccc gaaaatagat tggcgatcat gcgggatgag cgtgtgggcg catatgccct
     5101 ggcaggagga atcttattgt tgttgctgaa attcagcgca ctgctttcca tgccagagcg
     5161 ttgggctgca ctagtcatcg caccgacctt gagtcgctgg ggtatgagcc tggcaatcgt
     5221 cgctttcccg tatgcccgat cagagggatt ggggcgggtt atgaaagatc acgctacctg
     5281 gctgcatgcc agcgcagccg cattgacagc cctggccatc gcctggttct atgggcaacg
     5341 gagcggattg attctgcttg cagtatctgc tctcaccgta atcctggtag cgcgctttgt
     5401 tttaaagcgt attcccggct tgaccggcga tatctatggc gcgctcaatg agcttctcga
     5461 ggtcttgacc ttactcacat gggaaatctt acattaatcc ttggcggggc gcgcagcggc
     5521 aagtccacct ttgccctaaa tctggcggaa aaaatcaggg ggtgtgtggc attcatcgct
     5581 accgctcagc ccggtgaccc ggagatggca gctcgtattg ctgctcacca acgccagcgt
     5641 ccagccgatt ggcgcacgct ggagattccc catgcgatcg ccgcaacttg gctggggcaa
     5701 gaaatagatg ccgacctgat catcctggac tgcctgaccc tgttggtttc taacctggcc
     5761 gtaatggcct ctcccgatgt agatcatcca aatgagggga aattcaccga gctggtatgc
     5821 agcgagatca acacgctgat cgacctgatt caaaacagcc ccggggattg gattgtcatt
     5881 tcgaatgagg tggggttagg cctggtgcca ccatatccat taggtcgtat ttaccgagat
     5941 tggctgggtt gggccaatca gcgcctcgcc actgtcgctt ctgaggttta cctgctggtg
     6001 gcgggtatac ccgtgcccat tcacaactat aggttatgaa atgcccagga gaaaacctga
     6061 ccgcaacccc atctagttag ttatctttcc tgccgcggct tcgtccaaca accacactaa
     6121 ttccccctct accggattaa ccctttgcac gggcaaaacg agggactttt ccggggttgt
     6181 tagcgtcgca gccactgtag acgctttggc ttcgccggca acaatcagag ccacgcggcg
     6241 ggcggcgttg atgaccggta aggtaaccgt cacgcggctt accaatggtg gcggcggttg
     6301 atcatgatcc acagccacga cccagcgcgc agtttcatta attgccgggc ttcctgggaa
     6361 gagcgaggcg atatgaccat cctcccccat gcccagcaag atcaagtcga agtgagggaa
     6421 agctgtttgg ctgccgaaat agcttgaaat tgcaatctca taactggcag ctgcctgatc
     6481 cggcggtatt tcaccccgaa tgcggtgaac gttttcggca ggcagattta ccttagagat
     6541 caatacctcg cctgccatgc ggtaattgct ataagggtga tcaggtggta cacagcgctc
     6601 atcgccccag aagatgtgca cctgagacca gtccactgcc tgcttatacg gcgagctagc
     6661 aagcagagtg tacaccgctc tcggcgtgga gccaccggat agcatcatgg cgaagcgatc
     6721 ctgcaccgcc agcgcctctt gcatggctga taagatgtag ccggcagccc caccagctaa
     6781 cacctcaata ttctgaaata cgtgggtctt tgaattaata gccgaagcgc tgctctttcc
     6841 agacatctgc ctcgttatgg tagcctgcct gctcccaaaa acccagccgg tcttgcgcca
     6901 tgaactctag tccgcgaatc catttaccac ccttccagaa gtatggcgtc gtaaaatcgg
     6961 ttccagggat gttgcccacc acgcctctta gcgggtagcc gtggtctggc tcgagtggct
     7021 caccattgaa gtgtgtcgcc agcagaaaat taggcgccaa tgccacctcg agagggatat
     7081 tcacagtaaa tccatattcg caatgctgca tcaggtgccg tgcagttggc ttgagtttga
     7141 tatagccctg atcaatcagg gttttgatcg agacaccctc ccactcggta tcaaatttgc
     7201 tccagcgtgt cacgcagtgg atatccatag atatcttggt gcgaggcagc ttattgaact
     7261 catcccagtt ccaaaccacc tcctcttcga cctctccaaa tacacgcaaa tcccaggtcg
     7321 cggggttaaa acgcggcgtt gggccataat gcaacaccgg gaaacggttg gttaatgact
     7381 gtcctggtgg taagcgaccc tcctgcttga tccgttcttc atcatctcga cgtccaaaaa
     7441 cgcttttaag cataacaacc tccacaatta ttgcatgagt tgggcattca ttgcttcatt
     7501 tcaatgagct cgatacgccc cgcaaaagac acagtaaata caaaatgcca aggcgacggt
     7561 taatacagca gtcatgtcgt ttgacagcga ttatctcttt gctgtgtaat tacttagaca
     7621 accttctcta attggattat acctgtcaag aaagaacttg tgggcacaca gccattaaca
     7681 aaatattaca atctatcgta aaaatcgaga ttttgtactc gattttattc ttgggattaa
     7741 gcgtaaatca attaagctac aatagttgtt gagactagcg aattcatcac aaacatttgc
     7801 atatacaagg tggttatgat aaacttagaa aatagttgca agtgaggagg aagctatgtt
     7861 atatgaagac cgcatccgtc gtgaacgagc agagatgtcc aaaagcttcg ccaagctggc
     7921 ggattttttg cttaattcat acatagaagc cgcattcatg accgccagcg aacttgccca
     7981 caccctggat ttagacgcag ctacggttgt ccgcttttca caatttcttg gctactctgg
     8041 cttccccgag ctacaacgcg aaatacgcag ccgcgtgaaa aaagacctgc tgatccgccc
     8101 taagcaagcg gaggacgaca attcaacacc agggattgtc tccagagcga tgcaggagtt
     8161 aaatctggca ctcgaacaga cacgcatcac cctggatact gatgccgtgg aacgccttgt
     8221 ggaacagatt ggcaacgtca ggcggaccgt ggtgctagcc gaaggccctg cccaacccac
     8281 tgcctacagc ctggtgcact actttgagca ggggggtttt cccatatata ttgcacgacc
     8341 cggcatcgct gacctggcgc gcaccgtcaa taccgccacc tctcaagacc tattcctggc
     8401 aattgaggtt gcgggacaat ccccttatat cgcacgcgcc ttgggcgaag ccaaggcaaa
     8461 aaacatccct accgcagcta ttgtaggcgc ttcatctctg gcttccgcac gtgccgcgga
     8521 tatcgtactc gcagcacatg ctcacccctc attgggggtt ggcatactaa ccatcgaggc
     8581 aatcgtgtac gcatttgccc aggtgctgcg cctgcggtat gccgataatt ttgttggaac
     8641 cgaacaagcc atcgcggaac tcaccgcgcg aattcaaaca aacaccgatt aatactatgc
     8701 gcatcatacg ctaccaaacc aagaacgacc ccccacaata cggctgggtg cttgaaaatg
     8761 cagttggagc cattgaaggc gaccccttcg gagaattccg gcgcctggaa gctaaccaaa
     8821 atctcgactc ggtgcgtcta ctggctcctg tactgccagg gaagatcatc tgcgttgggc
     8881 gcaattacgc agcccacgcc cgggaacacg acgctgaagt tccagaggtg cccctgttat
     8941 tcctcaaagc gccttcagcg gtgattggac caggcgataa aattgtcctt ccaccccaat
     9001 cgcagcaggt cgagcatgaa gccgagctag cagtggtcat aggtcggcgc gggcggtgga
     9061 tatcccccga ggaagccctg gaacacgtca tggggtatac tattggcaat gatgtcaccg
     9121 cacgcgatct ccaatttcgt gataaccagt ggacgcgcgc caaaggcttt gacactttct
     9181 gtcccctagg accctggatc cagactgatt ttgaccctac cgacactctc atcacctgcc
     9241 atgtcaacgg ggagatgcgc caaatggctt ccactcgaga tatggtcttt agcgtccgcc
     9301 agcttatcgc atttacatct tccatcatga ctttggagcc gggtgatatc atcatgactg
     9361 gcacgccgtc tggggtcagc ccgttacagc ccggagatac ggtcaaagtc tcgattgaag
     9421 gtttgggtga attggagaat agcgtagtct cccaaccacc tcgctaatgc ccgaaggaat
     9481 tcgtcttccg atatctctcg atatccatac cgcagtccag accgcactgg tcattgtggg
     9541 actgctgatc gtgctattct tctggctagg gattcgctcg atccgcactg cacggcgtct
     9601 gcgcttcttt cgaatgcgcc gcgaccgaat ggtgcgcggc tggcgtttga tcatcaccgc
     9661 catcatcttg atccctatcg ctatcctgtt gaatcgtcgg ttggaacctt tgatatatac
     9721 ttattaccaa cctacggtca cggtaacgca aacgcctact atcacgatta cacccaccat
     9781 aagtcaaacg ccaaccatta ccctgacacc aaccatcacg ctgacgcctc tcgtctctaa
     9841 cacaccgaca atcacaccca cgccatttat accgcccgca atagaggctc gctttgaaag
     9901 cacgatcaca cccaaccctg aatcagtatt cagcccgcta accttcactg atgggctgga
     9961 tgctctctac cgacccctca aggagggcac agtttttgaa aatcccgtcg agcatatgta
    10021 cgctgtcttc agctatgatg gcatgattag ggggtcgcaa tggacagctt tatggtatcg
    10081 taacggggag ctggttcatt ttgagacttt accttgggat ggcgagacgg gtggtctggg
    10141 ttacacagac tggcagcccc aacccgaaga atggtttacg ggaacttacg aggtgcagat
    10201 attcgttggt agggattgga aggtatccgg tactttcacc gtagaaggtg aaccacctac
    10261 accagtgcca accgccacgc caaccaccac gccgacctcc acatacacac ccacgtcaac
    10321 aaaaactcgt cttccaaccc gcacgtacac acccatcccc ccaacgaggc caccctacat
    10381 tagccccaca ccgacaaaaa ccccgacccc ctggcccaca ttgacgcgca cgcctatcac
    10441 cccaagcatc accccccagc caacccgaac cagaacagtt accccgtcac cctagctcgt
    10501 gatttaatta tccaagagtt ctgcacaacc cgtctctttt tctactgctt caaatcccca
    10561 cttgaaaatt aaacgatccc ctcccgctcc atgcgggata gggcgcgggc gatctggctg
    10621 gcgacattgg cgttgtttaa cagcaagcca agattggcgc gcaagctgga tccctcagtc
    10681 aattcggata ccctgctcaa caaaaagggg gtaacttctt ttccggttat cttttctgtc
    10741 tgagctgctt ttagagccgc tcgcaccgct tttttcactg cctcgctagg caaggcgact
    10801 tcatctggcg gtggaacagt gaccagtatg gcgcttttca taccgagtgc ccaatgtgag
    10861 cgtgccacga atgcaacttc ctcgggagta tctgcgcgag tgctggtttt cagcccgcta
    10921 cggctagaat aaaaagcggg gaaatcgtct gtctggtagc caaccactgg caccccccag
    10981 gtttctagat attccaaagt tgcttgtaga tcaaggatgg ctttggcgcc cgcgcacaca
    11041 acaatcatgg ggatgttcgc cagggcgggt aaatctgcag agacatcaaa agctcctttg
    11101 cgccgggagc tgagctcaaa atgcacccca ccaatacccc cggtagcaaa aacatgtatc
    11161 ccaaccgcgt gggcagcata catggtgcca gccactgtcg ttccaccgct agccagggaa
    11221 gcaatcgccg gggcgaagtc acgcaggcta attttgagca attcaccacc tgaggtcgcc
    11281 aaaaagctca attgagctgg caccataccc acctgcacgc gtccatcaat cacgccgata
    11341 gttgcaggtg tgacgccctg gttgcgcact tcatcctcca tattatctgc cagagtgagg
    11401 ttatcgggat atggcaaacc gtgggtgatg acggtggtct caagggctac caaaggaaga
    11461 ttaagctcca aggcgtgcgt aacctcttga gtgagcgaga aatatggggg caattttttt
    11521 atcttcgagg agctagataa ccaattggtc atacaatttt tccagggaca aatccggaac
    11581 cactgcgccg cggaaacgta acgtgagaga tgccgcagaa acaccaagcc gcactgcatc
    11641 atcaagtggg atgtcgttta acaatgcaaa aatcacagtc gccgtgagtg catcaccagc
    11701 acccgttgga tctaaaatcg atgtcttgat ggctgggata taaccagaac tctccgaagt
    11761 cgcataacat aacccttgct ctgccatggt gatgataacg atatctacac cctgtgatat
    11821 gagatactta gccacctcta acgcctggcg acgtccaccg actggcgaag ttcgctcgga
    11881 taatactgcg gcttcagc
//
LOCUS       DBNGSX010000187         3358 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_568520, whole genome shotgun sequence.
ACCESSION   DBNGSX010000187 DBNGSX010000000
VERSION     DBNGSX010000187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_568520"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..1045)
                     /locus_tag="AC16CB_05895"
     CDS             complement(203..1045)
                     /locus_tag="AC16CB_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342034.1"
                     /translation="MNTDQKDTGGLPIRSNLTLAYIISLVIAALMTIASLAGLLYQTV
                     IYPTDELVQSFVPNDVVNLLIGLPTLLGAMWLTRRGRLIGLLLWPGALFYVFYTYIIY
                     TYSMPLNAGLLMHLALVLLSTYSMIGLVSSIDGKAVQNRLSGAVPEKAGGGVLVGLGV
                     LFLIRVIGVLVNAIINQTPLAATELALNISDFLITPTWIIGGVLLWRRNTPGYVFGLG
                     LLFQASMAFVGLIFVLILRPLITTAQFSLSDVLIVIVMGMICFVPFGLFIRGAASDRA
                     PSSV"
     gene            complement(1138..2295)
                     /locus_tag="AC16CB_05900"
     CDS             complement(1138..2295)
                     /locus_tag="AC16CB_05900"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF025053.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR3342035.1"
                     /translation="MIPAERAHLRVTAVSHPGMSGKNNEDRYGVSAYYTDAERETPAV
                     IAVVADGIGGHRAGEVAAEIAVETISAWVAESDASQPVNTLRHAIIHTGQVISEMAES
                     NSTLEGMGSTCACALIIGDQLYIATVGDSRIYLIRDNTIQLMTTDHTWVQEAIENGLL
                     GRDEARNHPNAHVIRRYLGSRQDVIPDTRLRLRSNESDSQAEENQGTRLRPGDILVLC
                     SDGLTDLVEDDEILEVLKTRNNQGALEDLVKMSNRRGGHDNVTIVTLAVPDVERPTTP
                     LIERQARKRLRSILIVAATLVLATITLLGGMLWYFARSDQEPTPMPSATQQVQATLFP
                     DQATQPGLFPTEAGIPGSASTALPGATVSTLAPPTPILATLTPWPTNTGAP"
     gene            complement(2292..>3358)
                     /locus_tag="AC16CB_05905"
     CDS             complement(2292..>3358)
                     /locus_tag="AC16CB_05905"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR3342036.1"
                     /translation="IRHGEQVITSPSLTFDFELSPPDPAFIFPSLEISRSIPAERRKN
                     FWEQVNPVDLKPDEQEIQVLIDFPDGRRRPLVRTTLYVDGVVVEENNEPPFEYFRWDL
                     SAYTDSSQHILQVEAVDSLGMNGTSIELPVQVNIDLPKPNPLSALFVNWPVLAGLAAL
                     LAGAIALLTLILTGRIRPHRLATFGLSGRRLIRKAKPPQPDATRAQRPRLETGGRYLP
                     NWVNRLQWPQRRLNPNAYAYLTYLSDAVEKHGATPISITADEITFGLDPSLATQVLED
                     PSVEGLHARLLRREDGSILLVDQGSVAGTWVNYTEVPEDGRVLEHGDLIHFGRVGFRF
                     TERHPQRARKLVITIEEPLS"
BASE COUNT          709 a          905 c         1003 g          741 t
ORIGIN      
        1 ggaagggtca tgttcctggc ttttggggat gaggtgctct acgccgggat gccgtagatt
       61 tccctagtct aatatattta tatccatcct gctattggcg tgtttgtgag catcttgtcg
      121 tctctagcca ggatcacttc aggcattttc ctatcatgct accactcagc cagtggtttt
      181 gctgccctct cgctattcta agctacacgg acgatggggc gcggtcagaa gccgcgccgc
      241 gtatgaaaag accaaaagga acgaaacaga tcatgcccat gacaattact ataaggacat
      301 cacttagcga gaactgcgct gtggttatga gcgggcgtaa gatcaatacg aagatcaatc
      361 cgacaaatgc catgcttgcc tgaaagagta accccaggcc gaaaacatac ccaggcgtat
      421 tgcgccgcca aagcagcact ccaccgatga tccaggtcgg ggtgatcaaa aagtccgaga
      481 tattaagcgc cagctccgtc gcggcgagcg gagtctggtt gatgatggca ttaaccaaaa
      541 cgccgatgac ccgtataaga aacaggacac caagcccaac caggacgccc ccgccagctt
      601 tttctggtac agcgccgcta agccgatttt gtaccgcttt cccatcgatg ctggagacaa
      661 gaccgatcat cgaatacgtg ctcaacaaga ccagcgcaag gtgcattaag agccctgcat
      721 tgagcggcat gctgtaggtg taaatgatgt aggtgtaaaa cacgtagaat agcgcgcccg
      781 gccacaagag cagcccgatg agcctgccgc gccgcgttag ccacatcgca ccaagcaggg
      841 taggcagacc gatcaggaga ttaactacat cattgggcac aaatgactgg accaattcgt
      901 ccgtggggta gatgacagtc tggtacaaga gaccggctag ggacgcaatc gtcatgagag
      961 cggcgatcac cagcgagata atgtatgcga gcgtgaggtt acttcggatc ggcagaccgc
     1021 cagtatcctt ttgatctgtg ttcatctatt atccagttcc ttatagtcag gttgctgctt
     1081 tatgagtagc ccctatcagc ctgggactga ggtagcgtgt ttcgcacctc gcacgtctta
     1141 cggcgcgccg gtgttggtcg gccaaggggt gagcgtggct aagatgggtg ttgggggggc
     1201 tagggtggag acagtagcgc ctgggagggc tgtcgaggcg ctgccaggaa tgcctgcctc
     1261 ggttgggaag agacccggct gcgtggcttg atccgggaag agggtcgcct ggacctgctg
     1321 ggttgccgat ggcatggggg tgggctcctg gtcgctacgc gcgaaatacc aaagcatgcc
     1381 acccagcagt gtgatggttg ccaggaccag cgtggcggcg acaatcaaga ttgatctcaa
     1441 acgcttgcgt gcctggcgct caatcagcgg ggtggttggg cgctcgacgt ccggaaccgc
     1501 caatgtgacg atggtcacgt tgtcatggcc accacggcgg ttgctcatct tgaccagatc
     1561 ttccagcgct ccctggttgt tgcgcgtctt aagtacctcc aggatttcat cgtcttccac
     1621 caggtcggtt agcccatcgc tgcacagcac caggatatca cctggccgca ggcgggtgcc
     1681 ctggttttcc tccgcctggg agtcgctctc attggaacgc aagcgcaggc gcgtatctgg
     1741 gatcacgtcc tggcgagatc ccaggtaacg gcgaatgacg tgcgcgttgg gatggttgcg
     1801 tgcctcgtcg cgtcctagca gcccattctc gatggcttct tgcacccagg tatggtctgt
     1861 cgtcattagc tggatggtgt tgtcacggat gagataaatg cgcgagtctc ccactgtggc
     1921 aatatatagt tgatcgccga tgatcaaggc gcaggcgcaa gttgagccca taccttccaa
     1981 ggtcgaattg ctttccgcca tttcgctgat cacctgaccg gtatggatga ttgcatggcg
     2041 cagggtgttc acgggctggc tcgcatcgct ctcagctacc caagcgctga tggtctctac
     2101 agcgatctca gccgccacct caccggctcg gtgcccgccg atgccatcgg caaccacagc
     2161 aatcaccgca ggcgtttcac gctccgcatc ggtatagtag gctgaaaccc cgtagcggtc
     2221 ttcgttattt ttaccgctca tgcccgggtg gctgacagcc gtcactctca ggtgggctct
     2281 ttcggcgggg atcacgatag gggctcctcg atggtaatta cgagcttgcg ggcgcgctgc
     2341 ggatgccgct cagtaaagcg gaaacccacc ctcccgaaat ggatcaagtc gccatgctcc
     2401 agcactcggc cgtcctcggg cacctcggta tagttgaccc atgtgccggc caccgaaccc
     2461 tggtcgacca gcaggatgga gccatcctcc ctacggagca ggcgggcatg cagcccttct
     2521 acagatggat cttctagcac ctgggttgcc aggctgggat ccaggccaaa ggtgatctca
     2581 tcggcggtaa tggagatggg ggttgcgccg tgtttttcga ccgcgtcgga gagataggtg
     2641 agataggcgt atgcgtttgg gttcaatctg cgctgcggcc attgcaggcg gttgacccag
     2701 ttgggcaggt agcgcccacc cgtttcgagc cgggggcgtt gagcgcgggt tgcatcaggt
     2761 tgagggggtt ttgccttgcg gatgagacgc ctcccggaaa gcccgaaagt cgctagccgg
     2821 tgagggcgga tgcgcccggt caggatcaaa gttagcaagg cgatggcacc tgccagcagg
     2881 gctgccagac ctgccagaac tggccagttg acgaataagg cgctcaaagg gtttggcttg
     2941 ggtaggtcaa tatttacttg tacaggtaat tcgatgctgg tgccgttcat gcccaggctg
     3001 tccaccgctt caacctgcaa gatgtgttga ctgctgtctg tataagcgct caaatcccag
     3061 cgaaaatact cgaagggagg ctcgttattc tcctccacca ctaccccatc cacgtacaat
     3121 gtggttcgta ccaacggacg cctgcgacca tcggggaagt cgatcagtac ctggatttct
     3181 tgttcatctg gctttaaatc gacagggttg acctgttccc agaagttctt tcgtcgttcg
     3241 gctggtatgc tgcgggagat ttccagagat gggaaaataa aggcgggatc gggagggctc
     3301 agttcgaaat cgaaggtcaa ggagggagaa gtgatcacct gttcgccatg gcgaattt
//
LOCUS       DBNGSX010000188         4992 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_222072, whole genome shotgun sequence.
ACCESSION   DBNGSX010000188 DBNGSX010000000
VERSION     DBNGSX010000188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_222072"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="AC16CB_05910"
     CDS             complement(<1..612)
                     /locus_tag="AC16CB_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3342037.1"
                     /translation="MQKRLILFILLILILSGVPGSLAAPAPAWTSKVDPWVLQTSQDG
                     ETEFLVFLTEQADLSAAAGLKSKTEKGAYVYQTLTEVARRTQPGLLGVLRKMGIPHRS
                     YWVANMVWVRGGLSAIQSLAQRPDVARLAANPRLRLELPSVQPDVLKEGEAVEAGSWN
                     IIKINADDVWALGYTGQGAVVGGADTGYAWNHPALKRQYRGWDG"
     gene            complement(645..1169)
                     /locus_tag="AC16CB_05915"
     CDS             complement(645..1169)
                     /locus_tag="AC16CB_05915"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HIR3342038.1"
                     /translation="MKLTGKHIAYLLEEGFEDLEFWVPLMRLREEGARVTIVGSGKGE
                     RFTGKHCLEAKVDVSAEQVQSADFDAVVVPGGWAPDKLRRYPAVTGLVREIYQQGKIV
                     AAICHAGLVLISAGIVKGHKSTGSLGIKDDLVNAGAIWVDEPAFRDGNLVWGRVVEDI
                     PDFCRELVKALSGE"
     gene            1338..1529
                     /locus_tag="AC16CB_05920"
     CDS             1338..1529
                     /locus_tag="AC16CB_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342039.1"
                     /translation="MTTLLIVLAVLIFMGIPALLFIAACIAGARASQRQEEFISYHEV
                     PASQEPVPDWMVIGEKAEA"
     gene            complement(1557..3053)
                     /gene="murJ"
                     /locus_tag="AC16CB_05925"
     CDS             complement(1557..3053)
                     /gene="murJ"
                     /locus_tag="AC16CB_05925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR3342040.1"
                     /translation="MGYTIGMSHIARSSLIIAVFFGLDKILGFLRQVFFNRQFSAAER
                     DVFFVSNNIPDLLSALISGGALGIALIPILSEFLERDGRQAAWDLFSRIVNLAFLVTA
                     CISVVIILLAEPLVRHVIAPGFNDPAKWALTASLMRLDLMAILIFSISGLVMAGLQAN
                     QHFLLPAMAPGFYNLGQIFGIMVLGPRLGIFGLVYGVILGALLHLAIQLPGLLRFGYR
                     WAPAIDLRNPGVQQVLRLMGPRLLTMLCLQSYFLARDRLASFFEEGAVSALNNGWFIQ
                     QVPETLIGTAIAIALLPSLSEHFVRGQTDDFRQTVNRALRAMLALTVPIAALLAVSIR
                     PLVRIAFDFEPRETEMVVWATRAFLLGLMGHTWLEVAVRSFYARQNATTPLLAAFLQA
                     VAYILLAVSLSRTVGHIGLALADTFSFSGQALLLLFLLNRRFPGVLQLGGTLWRAGLG
                     ALIGGGLTYALIQMLPLPILPLSLGALATGGLVVLPFIWSEVKLLVRL"
     gene            3131..4138
                     /locus_tag="AC16CB_05930"
     CDS             3131..4138
                     /locus_tag="AC16CB_05930"
                     /inference="COORDINATES: protein motif:HMM:NF027484.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HIR3342041.1"
                     /translation="MPLKKILNFVLLSSLLVFSGCKPGGQAEATETPLSPEAVFTAAA
                     LTAQVRMTELIASTPSPQPATPTQTIAAATVTSTVPSAGAAPSLTLPPAVPGADKVEF
                     VADLTIPDGTVFSPGEKFTKTWRLMNAGTATWTTAYTLVFFSGEQMGGAASTALPLEV
                     PSGQTLDLSVQLTAPAQAGSYTGYWILRNAAGRNFGLGPNADGPFYVQINVSGTAATG
                     TPQTGTPSASSTPGGDTVSGVTLGVDAATVQQACPYTFTFSGRFTLNKTATVTYRLEA
                     DTSFPINLPGATTTSLNAGTHEVTYTLEFSDSVTGTARLHVTAPQDVNSDPVSFSLTC
                     N"
     gene            4199..>4992
                     /locus_tag="AC16CB_05935"
     CDS             4199..>4992
                     /locus_tag="AC16CB_05935"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:NF019931.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HIR3342042.1"
                     /translation="MSLTQAIKQQAQTLGFQLVGITTPDPPPHYPAYEGWLQAGRHGE
                     MGYLASESARQRRRDPRQILPECRSILVLGIRYPAPTHPFPGGKEQIDIPLVKGSVAA
                     LDQRTSFPLPKVRGEGVGGGGYQQNAGRVASYAWGDDYHNVLATRMRSLVAFIETQLG
                     GPFPNRWYTDTGPILERDLAQRAGLGWIGKNTCLIAPSLGSYFFLAEILLGIELEPDP
                     PFLPDYCGLCTRCIEACPTACILPDRTLDATRCISYLTIELKGVIPL"
BASE COUNT         1194 a         1578 c         1241 g          979 t
ORIGIN      
        1 cccatcccaa ccccggtatt ggcgtttcaa cgcaggatga ttccaggcgt agccggtatc
       61 cgcaccgcca accactgcgc cttgccccgt gtagcccagc gcccatacat catcagcgtt
      121 gatcttgatg atgttccacg agcccgcttc caccgcttcg ccttctttta gcacatccgg
      181 ctggacggaa ggcagttcca aacggaggcg cggatttgct gccagcctgg ctacgtcagg
      241 gcgctgtgcc aggctttgga tagccgagag accaccccgc acccagacca tattcgctac
      301 ccaataagat cggtgtggga tgcccatttt acgaagcacg ccaagcaacc caggctgtgt
      361 tcgccgggca acctcggtca gcgtctgata aacgtaagcg cctttctctg tcttgctctt
      421 caacccagcc gcagcactca ggtcggcctg ttctgtcagg aagaccagga actccgtctc
      481 cccatcttgg gaggtctgca gcacccaggg atccaccttg cttgtccagg caggggcagg
      541 tgctgccaag gaacctggga cacctgataa gatcaagatg agcaggatga atagaatgag
      601 gcgcttctgc atatcattaa cccatcacaa ttgcccctcg ctgcttactc tccactcaat
      661 gccttcacca gctcgcggca aaagtcgggg atatcctcca ccacccgccc ccacaccagg
      721 ttaccatccc tgaaggctgg ctcgtcaacc catattgccc cggcattgac caggtcgtct
      781 ttgataccca gcgagccggt agatttatgc cccttgacga tgccagcgga gatcaaaacc
      841 aacccagcgt ggcagatagc agcgacgatc ttcccctgct ggtatatctc gcgcaccaga
      901 ccggtcactg ccgggtagcg gcgcaactta tccggcgccc aaccgcccgg cacgactacg
      961 gcgtcaaaat cagcagactg cacctgttcg gcagatacat caaccttcgc ctccaggcaa
     1021 tgcttgccag tgaatcgctc acccttgccg ctgcccacaa tcgtcacccg agcgccctcc
     1081 tcgcgcaggc gcattaacgg cacccaaaac tccaaatcct caaatccttc ttccagcaag
     1141 taggcgatgt gtttccctgt cagtttcatc ttccctccat cttcctgttt tataaaaata
     1201 gccttataat accggtctaa aatcaccgga aatagcagga aatgttaaaa aacaaataaa
     1261 atccatttat ccttgcatta aagtttgttc actaaataaa ttttgcatgt ataatctcac
     1321 tacaggagat tacaaagatg actaccttat taatagtttt agccgttctc atatttatgg
     1381 gaatacctgc actccttttc atcgctgcct gcatcgctgg agcaagggca agccagcgcc
     1441 aggaagaatt tatctcttat catgaagttc cagcctctca ggaaccagta ccagattgga
     1501 tggtgatcgg ggagaaagca gaagcctgat cttgtaatag gcaattcctg ataacactat
     1561 aacctcacta acaacttaac ctcactccag ataaagggca gcacgacgag ccctccggtt
     1621 gccagcgccc ccagcgagag cggcaatatc ggcaagggta gcatttgtat caaagcatag
     1681 gtcaggccgc caccaatcaa ggcccctaaa ccagccctcc acaaagtccc ccccagctgc
     1741 aaaacccctg gaaagcgacg attcaacaag aaaagcaaaa gcaatgcctg tccgctgaac
     1801 gagaacgtat cagccagcgc cagaccgatg tgtccaacgg tcctcgagag cgatactgcc
     1861 agcaggatgt aggcaaccgc ctgcaaaaag gctgccagca agggtgtggt agcgttctgc
     1921 cgcgcataaa atgagcgcac tgccacctcc agccaagtgt ggcccatcaa ccccagcaag
     1981 aaggcgcgcg tcgcccacac caccatttcc gtctctcgcg gttcaaagtc aaaagcgatc
     2041 cgcaccaagg gacggatgct caccgccagc agcgcagcaa taggaacggt caaagccagc
     2101 atggccctca aggcacggtt taccgtctgg cggaagtcat ccgtctgccc gcgcacaaaa
     2161 tgttcggaga gggacggcag cagggcaatg gcgatggccg taccgatcaa cgtctccggc
     2221 acctgctgga tgaaccagcc attattcagc gccgagacag ccccctcttc aaagaatgaa
     2281 gccagccgat cgcgcgccag gaaataactc tgcaggcaca acatagtcag caggcgcgga
     2341 cccatcaggc gcagcacttg ttgcaccccc ggattacgca gatcgatggc aggcgcccat
     2401 cgataaccaa agcgaagcaa acccggcaat tgaattgcca ggtgcagcaa cgcccctaag
     2461 atcactccat agaccaatcc aaagataccc aggcgtggtc ccaacaccat gataccgaaa
     2521 atctggccca ggttatagaa ccctggcgcc atcgccggca gcaggaaatg ctggttggcc
     2581 tgcaatccgg ccatcaccaa cccgctgatc gagaagatca ggatcgccat caggtcaagc
     2641 cgcatcagac tggcagtgag tgcccacttg gctgggtcat taaaaccggg cgcgatcaca
     2701 tggcgcacca gcggctcagc cagcaagatg atgaccacag agatgcatgc tgtgaccaga
     2761 aaagccaggt tgacgatgcg agagaacagg tcccaggccg cttggcgacc gtcgcgctcc
     2821 agaaattcag acaagatcgg gatcagtgca attcccagcg cgccaccgga gattaaggca
     2881 gagagcaggt cggggatgtt attggagaca aagaaaacat ctcgctcggc ggcgctgaac
     2941 tggcggttga agaacacctg tcgaagaaat cccaggatct tatccaaccc aaagaataca
     3001 gcaatgatca gcgatgagcg ggcgatgtgc gacatgccga tagtataccc cacctaaaca
     3061 actcccctgt gccctaattt caagcttacc agttatactt ggggcgtgga aattacctgg
     3121 aggtaagatc gtgcctttga aaaaaatact caacttcgtt ttattgtcat ctctcctggt
     3181 cttctcaggc tgcaaaccgg gggggcaagc tgaagcaacc gagacacccc tcagccccga
     3241 agccgttttc actgcggcgg cgctaaccgc ccaggtcagg atgactgagc tgattgccag
     3301 caccccctcc ccccagccag ccacgcccac gcaaactatc gctgcagcaa cggtaacgtc
     3361 tacagtgcct tcggctggtg ccgcacctag cctcaccttg cctcccgctg tgccgggagc
     3421 agacaaggtt gagttcgtcg ccgatttgac catacccgac ggcacagttt tctcacctgg
     3481 tgaaaaattc accaagacct ggcgcttgat gaatgccggc acagctacct ggacaactgc
     3541 ctataccctg gttttcttca gcggggagca aatgggtggt gcggcatcta ccgccttacc
     3601 cctggaggtg ccatctggac aaaccttgga cctcagcgta caactcaccg cccccgccca
     3661 ggctggttcg tacaccggtt attggatcct ccgcaatgct gccggaagaa attttgggct
     3721 gggacctaac gcagatgggc cattctatgt gcagatcaac gtctcgggga cagccgccac
     3781 cggcacaccc cagaccggca cgccatccgc ctcatccaca ccaggaggag ataccgtcag
     3841 cggtgttacc ctgggcgttg acgcggcaac ggtacaacag gcctgcccct acactttcac
     3901 cttttctgga cggttcaccc taaacaaaac cgccacggtc acttaccggc tcgaagcgga
     3961 taccagcttc ccgatcaatc tacctggagc gaccaccact tcactgaatg ccgggacgca
     4021 cgaagtcacc tataccctgg aattcagcga ttcggtcacg gggacagccc ggctgcacgt
     4081 cactgcccca caagatgtga attcggatcc ggttagcttc tcgctgacct gcaattaagc
     4141 tgtgtttttg gtctgtgggc atctatatct ctcatagtct atcgtttcat ttgcaaccat
     4201 gtccctcacc caggcaatca aacagcaggc tcaaacattg ggttttcaat tggttggcat
     4261 caccacgcct gatcctccgc ctcattaccc ggcctacgag ggctggttgc aagccggccg
     4321 gcacggcgag atgggctacc tggcaagcga gagtgcccgc cagcgccgcc gcgacccgcg
     4381 ccagatcctg cctgaatgcc gctcaattct ggtgctgggc atacgttatc cagcccccac
     4441 ccacccgttc ccagggggaa aagagcaaat agatatcccg ctggtcaagg gcagcgtcgc
     4501 tgcccttgac cagcggactt ctttccccct tcccaaggtt aggggggagg gggtaggggg
     4561 tgggggatac cagcaaaatg ccggccgtgt cgcctcttac gcctggggcg acgattatca
     4621 caacgtactg gcgacgcgca tgcgctccct ggtggctttc atcgagacgc agctgggagg
     4681 cccgtttcca aaccgctggt acaccgacac cggccccatc ctggagcgcg acctggcaca
     4741 acgcgccggt ctgggatgga tcggtaaaaa cacctgcctg atcgcaccca gccttggatc
     4801 gtatttcttc ctggcagaga tcctcctggg gatcgagctg gaacccgatc cacccttcct
     4861 gccggattat tgcggcttgt gcacgcgctg tatcgaagcc tgtccaaccg cctgcatttt
     4921 gcccgatcgc accttggatg ctacccgctg catctcgtac ctgaccatcg agctaaaagg
     4981 cgttatcccg ct
//
LOCUS       DBNGSX010000189         2829 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_291556, whole genome shotgun sequence.
ACCESSION   DBNGSX010000189 DBNGSX010000000
VERSION     DBNGSX010000189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_291556"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="AC16CB_05940"
     CDS             <1..641
                     /locus_tag="AC16CB_05940"
                     /inference="COORDINATES: protein motif:HMM:NF021010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HIR3342043.1"
                     /translation="FFCMGMQGGSVNIWHWKADWQADIAAWQDMETLYPDMNVDQYPF
                     AQGELPAPVDYQDVNYVPAMAAENLFAMPRLSPVEDLVAGGFGTLTAEPAEAQNVQGM
                     GVWENNRWRVIFSRDIISQDADDASFDSGQVGSVAFAAWDGANGERNGMKSTSQWVSL
                     QVEGKGPAVPGDTVPEQAAGLPVTLIMSILVGGLVILLIVGAIIYFRLPDEG"
     gene            646..1599
                     /locus_tag="AC16CB_05945"
     CDS             646..1599
                     /locus_tag="AC16CB_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342044.1"
                     /translation="MDRMPPFLRWLSIAALADWLVTRTLARSAIFMPKSQPLLLVYQT
                     LTLGGQLASVLSALLALLGVGWLAWRLRGELKGLISLVLLSLTAISLVTMAIPPSNLA
                     QAATQLLGLAAIFWIGLHAWRQATNPTQQAAVLLPGLALLIGRVYQTSQVVNAALDLA
                     DPPANALIFFNLGELLAVLSPIGIWWAFRRVGRGGLSLYLWSGIPALLFVVGYFLNPS
                     MTGILAIWSSGLTLYLPWPLYALSLWLFGFVLLAAWKQGSPAGVALLLLAAGGHAPQL
                     STQLFFGLVGLSLLTTRLPSSDFATYPAQAASASPHALEIG"
     gene            1758..2291
                     /locus_tag="AC16CB_05950"
     CDS             1758..2291
                     /locus_tag="AC16CB_05950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR3342045.1"
                     /translation="MGDITHEFTLPPDVEAVFKKFWTCEFTTLAKDGTPVTWPILPIY
                     QPGLRSFVFLTSIGTPQKVFNIRRNRRVSLFYSDPTGSDLVDPPAVLVQGDAQVGDQI
                     ITSREQIDEQLSLDMQEQGRRLLKHQPAITMYTNNRLTQYVMDWYFMRLLITITPRRI
                     LWWEHGDFTSPAHVIEV"
     gene            2293..2742
                     /locus_tag="AC16CB_05955"
     CDS             2293..2742
                     /locus_tag="AC16CB_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342046.1"
                     /translation="MWNRIARQVQSYSSAVLTFVEGNGYPFSVRCDPQYDRSQQVFRL
                     HIPNQIALQSGPAGLLCHQHDKNLWNLKSMVLRGILEEDDKGWFFRPLQFIPGMGGFF
                     GYVRFVAQGRRLARNYLERRGLDRPTIPWAEYATIVKLAKKDKFQIE"
BASE COUNT          586 a          692 c          799 g          752 t
ORIGIN      
        1 ctttcttctg catgggtatg caaggtggga gtgtcaacat ttggcactgg aaggctgact
       61 ggcaagccga tatcgccgcc tggcaggata tggaaacgct ttatccggat atgaacgtcg
      121 atcaataccc atttgcccag ggggagctgc cggctccggt tgattatcag gatgtcaatt
      181 acgtgccggc aatggctgca gaaaacctgt ttgccatgcc ccgcctgtcg cctgtggaag
      241 atttggttgc tggcggattt ggcaccctca ccgctgagcc cgctgaggca cagaatgtgc
      301 agggtatggg ggtttgggag aacaaccgtt ggagggtgat cttcagccgc gatattatct
      361 ctcaagatgc tgatgacgca tcttttgact ccgggcaggt tggttcagtg gcatttgccg
      421 cctgggatgg tgccaatggc gagcggaacg ggatgaagtc tacttcacaa tgggtctcgc
      481 tccaggtaga gggcaaggga ccagcagtcc cgggtgatac cgttccggag caggctgcgg
      541 gattgccagt aactttgatc atgtccattc tcgttggtgg gctggtgatc ttgttgattg
      601 taggagccat tatctacttc cgcttacccg atgaaggatg aactgatgga tcgcatgccg
      661 ccatttctcc gttggctgtc catagctgct ctggcggact ggctggtgac tcgcaccctg
      721 gcgcgctcgg ctatcttcat gcctaagtcg cagcccctat tgcttgtcta ccagacgctc
      781 acgctggggg ggcaattggc cagcgtgctc agcgcgctgc tggcgctgct cggagtaggt
      841 tggctggcat ggcgcttgcg tggagaactc aagggattaa tctccctggt tttactcagc
      901 ctgacggcca tcagcctggt cacgatggcg atccctccaa gcaacctggc tcaggctgct
      961 acccagctcc tcggcctggc ggctatcttc tggatcggtc tccatgcctg gcgacaggcg
     1021 actaatccaa cgcaacaagc ggccgtgctg ttgccagggc tggcactatt gatcggtcgg
     1081 gtgtaccaga ctagccaggt ggtgaatgct gcgctcgact tggcggatcc gccagccaat
     1141 gcgctgatct tttttaatct gggcgagttg ttggcggtgc ttagcccaat tgggatatgg
     1201 tgggcttttc gccgcgtagg gcggggtggg ctttctcttt acttgtggtc gggaatccca
     1261 gctcttttat tcgtagtggg atactttctc aatccatcca tgaccggcat cttggcgatc
     1321 tggtcaagcg gattgacttt atatttaccc tggcctttgt atgcgcttag cttgtggctg
     1381 tttggattcg ttttattggc tgcatggaag caaggtagcc ccgcaggtgt tgccttgctt
     1441 ctgctggcag ctggaggaca tgctcctcag cttagcacgc agttgttttt cggtttggtc
     1501 gggctgagtt tgcttacaac cagactaccc tcatcagatt ttgctactta tcccgctcaa
     1561 gcggcttccg cttctcccca tgcactcgaa ataggttaaa atttagtaca gcaacgttga
     1621 attacagaag aggggattat gcttggcgaa agccgtttgt actttcccat taataaccac
     1681 ctttataaca attgaaagaa tagctgtgta cttataatag gtggggtaat ggcaggattt
     1741 ttgcgggagg atgaacgatg ggtgatatca cccatgaatt tacacttcca cctgatgtgg
     1801 aagcggtttt taaaaagttc tggacttgtg agttcaccac gcttgccaaa gatggcacgc
     1861 ccgtcacttg gcccatcttg cccatttatc aacccgggct gcgcagtttt gttttcctga
     1921 cctctatcgg tacaccacaa aaggttttta atattcggcg aaaccggcgc gtctcgttgt
     1981 tttattccga tccaacgggg agtgatcttg tcgatccgcc agctgtgctc gtgcaggggg
     2041 atgctcaggt tggggatcag atcatcacct ccagggagca gatagacgaa cagttaagtt
     2101 tagatatgca agagcaaggg cgcagacttt tgaagcacca accggcgata actatgtaca
     2161 ccaacaatcg cctcacccag tatgtgatgg attggtattt catgcgtctg ctgattacga
     2221 tcacaccgcg acgcatttta tggtgggaac acggagattt tacttctcca gcacatgtaa
     2281 ttgaggtgta gggtgtggaa tcgcatcgct aggcaggttc aatcttattc cagcgcggta
     2341 ttgacttttg tcgaggggaa cggttaccct tttagcgtgc gctgtgaccc gcaatatgat
     2401 cgcagccagc aagtgtttcg cctgcatata ccaaatcaga ttgccctcca atctggccca
     2461 gccggcctgc tttgccacca gcatgacaag aacctgtgga atctgaaaag tatggtgctg
     2521 cgcggcatac tggaagagga tgataaaggt tggttttttc gaccgttgca atttattccg
     2581 gggatgggtg gattcttcgg ttatgtacga tttgtcgccc aaggacggcg tcttgctcgc
     2641 aattacctgg agcgtcgtgg cctggatcgc cctaccatac cttgggctga atatgccacc
     2701 atagtgaaac tagccaagaa ggacaaattc cagatagagt aagagggctt acccgagtat
     2761 aattaattta gaagttacgt agttggatac gtggcagggg ggtatgggcg ggcatcgccc
     2821 gcccatacc
//
LOCUS       DBNGSX010000190         3991 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1746140, whole genome shotgun sequence.
ACCESSION   DBNGSX010000190 DBNGSX010000000
VERSION     DBNGSX010000190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1746140"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..523
                     /gene="nuoI"
                     /locus_tag="AC16CB_05960"
     CDS             20..523
                     /gene="nuoI"
                     /locus_tag="AC16CB_05960"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HIR3342047.1"
                     /translation="MFSEIMRGLSTTFRSMFEKPVTIQYPEVKRPVRKRFKGRHQLHR
                     YENGLEKCIGCSLCAAACPADAIFVESSENSDEERFSPGERYASTYEINYLRCIFCGY
                     CEDACPTEAIVLGDQYELSFTNRRESIYAKEMLLVPLQEGGKPTPQKVEPGMFTRSVP
                     VMKDPMD"
     gene            616..1122
                     /locus_tag="AC16CB_05965"
     CDS             616..1122
                     /locus_tag="AC16CB_05965"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HIR3342048.1"
                     /translation="MMTNELILFLILALVAVVSALGMLFSRNAVYAALFLILNFGTVA
                     VFYLLLNAPFIALTQVTVYAGAIMVLFLFVIMLLGAEQSGRTPAVRWLRPVTISLGAI
                     LLAEAIYIIYTQRSLLPAKLTTSASFGTPNSVGEILFNQYLLPFEVTSVLLLVAMIGA
                     IVLTKPRT"
     gene            1139..1441
                     /gene="nuoK"
                     /locus_tag="AC16CB_05970"
     CDS             1139..1441
                     /gene="nuoK"
                     /locus_tag="AC16CB_05970"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HIR3342049.1"
                     /translation="MLPVAYYIGLSAVLFTIGALGVLIRRNSIVIFMSVELMLNAANL
                     AFVAFARAYESMSGQIFVFFVMTVAAAEVAVGLALIVAIFHSKQSIDVDQMNTMKG"
     gene            1446..3599
                     /locus_tag="AC16CB_05975"
     CDS             1446..3599
                     /locus_tag="AC16CB_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HIR3342050.1"
                     /translation="MLLSEATANAPQFFYLVPWIVFFPVIGLIINMAFGGRLGEKAIG
                     AVACLASGLAFVVSVMLAISLSGHPEGMVVPLAEWITIGELDLQWAFRVDTLSVTMML
                     VVSGVGTLIHIYAVGYMHEDVRHNGDPGRFRRFFVYFNLFIAAMMVLVSGDNYLMLFV
                     GWEGVGLCSYLLIGFWYEKGKDGIGNAIAGKKAFVTNRVGDFGFLIAAFIMFATFRTF
                     DFEAVFAKAEAVAEATPWVITAITLFMLLGVAGKSAQLPLYVWLPDAMAGPTPVSALI
                     HAATMVTAGVYLVARSHPLYVLVPDAQNVVAWVGAATALFAATIAVGQFDIKRVLAYS
                     TISQLGFMVAAVGMGAFVAGMFHLVTHAFFKALLFLSSGSVIQGIERGHHHVEHDPRL
                     KKQLKGHGHDFDPQDMRNMGGLRNRMKTTYWVYLIGALALAGIVPLAGFWSKDEILAE
                     ANHLNSGVYWLLTIAAFFTAFYMGRQILMVFFGEPRSPAAEHAEESPPLMTIPLIILA
                     VLSVLGGALNLPAVHTFGNWLEHTIHLAEAGGEAHTAEAAAAGGFNLTVALISTLLAL
                     AAIALSWWLYDRRTRELQALPAGKRPDDPLRALIGPIFTGMENKWWVDELYWAIILNP
                     YISLSRFLADVIDWRFWHDWFHDVVITGGYNGLSRLLSVRIDLGGIDAFANGLATATQ
                     NLAAQMRRTQTGYVRNYALSVFIGAILILGYLILR"
     gene            3690..>3991
                     /locus_tag="AC16CB_05980"
     CDS             3690..>3991
                     /locus_tag="AC16CB_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-binding domain-containing protein"
                     /protein_id="HIR3342051.1"
                     /translation="MNYLLHPLTLVTFFPLLGAVVILFLKAEQKNAARWVALVSSTIT
                     FGISIAMLAQFNSSNPGLQMVINLPWIQVAGWNIAFHMGVDGLSILLVLLTTFLTP"
BASE COUNT          750 a         1147 c         1111 g          983 t
ORIGIN      
        1 ataggtagga ggtaggtaga tgttttctga aattatgcgt ggtctctcga ctacgtttcg
       61 atccatgttt gaaaagccgg tgaccatcca gtatccggag gtgaaacgcc cggtgcgcaa
      121 gcgcttcaaa gggcgtcacc aactgcaccg ctacgagaac ggtctggaaa agtgcattgg
      181 ctgctcgctt tgcgcagcag cttgcccggc ggatgccatc ttcgtagaat catccgagaa
      241 ttctgacgag gagcgcttct ctcccggaga acgctatgcc agcacctatg agattaatta
      301 cctgcgctgt atattctgcg gctattgcga agatgcctgc cccaccgagg cgattgtcct
      361 tggcgaccaa tacgagctct ccttcaccaa ccggcgggaa tcgatatatg ctaaagagat
      421 gctgctggtg ccgctccagg agggcggcaa acccactccg cagaaggtcg agccgggcat
      481 gtttacacgt tcggtcccgg tcatgaaaga tccgatggat tgatatttgt aggttgatga
      541 ttgagaattg ctgatttatc catatcatta gcctgggcga caatcagcaa tctgcaatca
      601 gaaaccgagg tggacatgat gactaatgaa ttgatccttt ttctcatcct ggcattggtg
      661 gccgtcgttt cggcattggg tatgctcttc agccgcaatg cagtctatgc ggccctgttt
      721 ttaatcctca attttggcac cgtggcggta ttttatctgc tgctaaacgc gccattcatc
      781 gccctgaccc aggtgacggt ctatgctggc gcgatcatgg tgctcttctt gtttgtcatc
      841 atgctcctgg gcgcagagca gtccgggcgc acgccagccg tcaggtggtt acgacctgtc
      901 accatctcct tgggggcgat cttgctggca gaggccattt atatcatcta tacccagcgc
      961 agcctgctgc ccgccaagct aaccacttcg gcctcattcg gcacgccaaa cagtgtcggt
     1021 gaaatcctgt tcaatcaata cctgttgccc tttgaggtga cctcggtcct gctgttggtt
     1081 gccatgatcg gtgccattgt attaaccaaa ccaagaacgt gaaattccgg aggattgaat
     1141 gctccctgtc gcttactaca ttggcctgtc tgccgtattg ttcaccatcg gcgcgctggg
     1201 cgtgctgatc cggcgcaatt ccatcgtcat cttcatgtcg gtggagctga tgttgaacgc
     1261 ggccaacctg gctttcgtgg cattcgcccg tgcatatgaa tcgatgagcg gccagatatt
     1321 cgttttcttc gtcatgaccg tggctgctgc cgaggtagca gtcggtctgg cgctgattgt
     1381 ggccatcttc cacagcaagc agagcatcga tgtagaccag atgaatacga tgaaagggtg
     1441 acgaaatgtt actcagcgaa gcaaccgcaa atgcgcctca atttttctat ctggtcccgt
     1501 ggatcgtctt ctttccagtc atcgggttaa tcatcaatat ggcctttggc ggtcgcctgg
     1561 gcgagaaggc tatcggcgcg gtagcctgtc tggcttcagg gctggccttt gttgtctcag
     1621 tgatgctggc gatctccctc tccggtcacc ccgaggggat ggtcgtcccg ctggcagaat
     1681 ggatcaccat tggtgaattg gacctgcaat gggcattccg cgtggacact ctctcggtca
     1741 ccatgatgct ggtagtgtct ggggtgggca cgctgatcca catttacgct gtaggttaca
     1801 tgcacgaaga tgtacgccac aacggcgatc cggggcgttt ccgccgcttt ttcgtctatt
     1861 tcaacctgtt cattgcagcc atgatggtgc tagtgagcgg ggacaactac ctgatgttgt
     1921 ttgtcggctg ggagggtgtg ggactgtgct cgtacctttt gatcggcttc tggtacgaaa
     1981 aaggcaagga cggcatcggc aatgccatcg ctggtaaaaa agccttcgtc accaaccggg
     2041 tgggcgattt tggcttcctg atcgctgcct ttatcatgtt cgccaccttc cgcaccttcg
     2101 attttgaagc ggtttttgcc aaagcggagg cggtagccga agccactccc tgggtcatca
     2161 ctgccatcac cctgttcatg ctgctaggcg tggcggggaa atcggcgcaa ttacccctat
     2221 atgtgtggtt gccagacgcg atggcaggtc ccacgccggt ctctgccttg atccacgccg
     2281 ccacgatggt aactgctggc gtgtacctgg tggctcgctc acatccgctg tacgtcttgg
     2341 tgcccgacgc ccagaatgtc gtcgcctggg tgggcgcggc gactgctttg ttcgccgcca
     2401 cgatcgccgt ggggcagttt gacatcaagc gcgtgctggc atactcgacg atcagccagc
     2461 tgggcttcat ggtcgccgct gtgggcatgg gcgcgttcgt ggcgggcatg ttccacctgg
     2521 tcacacatgc cttcttcaag gcgctgctat ttctctcgtc cggttcggtc atccagggca
     2581 tcgagcgcgg tcaccatcac gtggagcacg acccacggct gaagaagcag ctaaagggtc
     2641 acggtcacga cttcgatccg caagacatgc gcaatatggg tggcctgcgc aatcgcatga
     2701 agaccaccta ctgggtgtac ctgatcggcg cgctggccct ggcaggcatt gtgcccctgg
     2761 caggtttctg gtccaaggac gagatcctgg cggaagccaa ccacctcaat agcggcgtgt
     2821 actggctgct caccatcgcc gccttcttca ccgctttcta tatggggcgg cagatcttaa
     2881 tggtcttctt tggcgaacca cgctcccccg ccgccgagca cgctgaggag agccccccgc
     2941 tgatgaccat ccccttgatc atcctggcag tcctttccgt actgggtgga gcgctcaacc
     3001 tgcccgccgt gcacaccttc gggaattggc tggagcacac catccaccta gctgaagcgg
     3061 gaggtgaggc acacaccgcc gaggcagctg ccgcaggcgg ttttaacctc acggtggctc
     3121 tgatctctac cctgctggcg ttggcagcta ttgccctctc ctggtggctg tatgatcgcc
     3181 gcacgcggga gctgcaggcc ctgcccgcag gcaagcgacc agatgacccc ctgcgggcgc
     3241 tgatcggccc gatcttcacc ggcatggaga acaaatggtg ggttgacgag ctctactggg
     3301 cgatcatcct caacccctac atatccctgt cgcgcttcct ggctgatgtg atcgactggc
     3361 gcttctggca cgactggttc catgacgtcg tcatcacggg cgggtataac ggcttgagcc
     3421 gcctgctctc ggtgcgtatc gacctgggcg gcatcgacgc ctttgccaac ggtctggcga
     3481 cggctactca aaacctggct gcgcagatgc gccgcaccca gactggctac gtgcgcaatt
     3541 atgcactgtc cgtatttatt ggagcaattt tgatccttgg ttatctaatc ttgcggtgat
     3601 ttttacccca ctatccctta tcctctcccc ctcgttacct ggggaagcgg ataggagata
     3661 agggatactt atttgggcga ggacaactta tgaactattt actacatccc ctgaccctgg
     3721 tcacattctt cccccttctg ggtgccgtgg tgatcctatt cctcaaggca gagcagaaaa
     3781 acgctgcccg ctgggtggcg ctggtctcat caacgatcac ctttggcatt tccatcgcca
     3841 tgctggcgca gttcaattcc agcaaccccg ggttgcagat ggtcattaat ctgccctgga
     3901 ttcaagtagc tggctggaac atcgccttcc acatgggcgt ggacggcctg agcatcctgt
     3961 tggtgttgct caccaccttc ctcacgccga t
//
LOCUS       DBNGSX010000191         2959 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1677513, whole genome shotgun sequence.
ACCESSION   DBNGSX010000191 DBNGSX010000000
VERSION     DBNGSX010000191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1677513"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="AC16CB_05985"
     CDS             <1..183
                     /locus_tag="AC16CB_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HIR3342052.1"
                     /translation="VMMQSQIFPLLFAKLQKMLNNYIQVPEILNRIEQYIVPPDLGSQ
                     AGVLGALALAERALPY"
     gene            296..2239
                     /locus_tag="AC16CB_05990"
     CDS             296..2239
                     /locus_tag="AC16CB_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR3342053.1"
                     /translation="MESLIDRQTKITFSRLLPRLKAHFKRQASKHPVAWSSFVRRLDE
                     HFPSLFNLYHQLYADQYDFFYHLEELLNTLARMWLQRPEYLKELDDSREANQDWFQSN
                     KMLGGALYVDLYAGNLNGLRQKIPYFKELGLTYLHLMPLFHVPKGENDGGYAVSSYRK
                     VNPKLGTMKQLARLAQELRENGISLVLDLVFNHTSNEHTWALQAREGSLDYQDYYRIF
                     PDRIVPDAYEKHLREIFPDEHAGAFTFWPEMKTWVWTTFHSYQWDLNYANPVVFNRMV
                     EEMLFLANQGVEVLRLDAVAFIWKELGTNCESLPKAHMLIQAFNAAARIAAPALLFKS
                     EAIVHPDEVIQYISPDECQLSYNPLLMALLWEAMATRKVGLLSQALRDRFAIPTHCAW
                     VNYVRCHDDIGWTFSDEDADRLGINGYSHRKFLNDFYTGRFPGSFARGLPFQENPKTG
                     DARISGTCASLAGLEKAVREETDVEVELAIRRILLIHGVILTVGGIPLIYLGDEIGSL
                     NDYSFRDDPGKAADSRWVHRMRVDWEKYARRSNSATLEGRVYQGICRLINLRLSRPVF
                     AGQDMQVLETDNENVLGYLRRHDNSIALIFANFSEREQTVSANLLRLYGLSYNTQDMF
                     SGAKLPQGDINLKPYDFLALAPQ"
     gene            2539..>2959
                     /locus_tag="AC16CB_05995"
     CDS             2539..>2959
                     /locus_tag="AC16CB_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR3342054.1"
                     /translation="MALLRAYLFRSIWVVTEDGRTLDLGSPTVRSLFAYLLLHRDHPS
                     DRRRLAFLFWPRASEAAARRNLRQYLHRLRRALEPVDPLGGLLIAEHSGVQLDPSAQI
                     WVDVEAFRHNTRPEATLPDLQTAAELYSGDLLEDVYED"
BASE COUNT          735 a          691 c          740 g          793 t
ORIGIN      
        1 gtaatgatgc agtcacagat atttccatta ctgtttgcca aactgcaaaa aatgctcaac
       61 aactacatcc aggtaccaga gatcttgaat cgcatcgaac aatatatcgt cccacctgat
      121 ttgggatcgc aagctggcgt cttaggtgcg ctcgccctag ctgagcgggc actaccatat
      181 tgatcagtgg gggatagaaa ttacctgggt tttgggtact tgccatgacg agggctattt
      241 atagcctaca cacagccata gagaatacca aagaaccgtt ggctttggag gatccttgga
      301 aagcttgatc gatcgccaaa caaagattac attctcacgc ctcttacctc gcctgaaggc
      361 tcattttaag cgccaggcat ctaagcatcc agttgcatgg tcaagtttcg tgcgtcggct
      421 agacgagcat ttccccagcc tgtttaactt ataccaccag ctttatgcag accaatacga
      481 ttttttctac catttggaag agttactcaa tacccttgcc cggatgtggc tgcaacgccc
      541 ggagtacttg aaggagctgg acgattctcg ggaagcgaat caagattggt ttcaatccaa
      601 taagatgctt ggaggcgcgc tttatgtgga tttgtatgct ggcaacctta acggcctgcg
      661 gcaaaaaatc ccatatttta aggagcttgg cctgacttat cttcacttga tgccattatt
      721 tcatgttcca aagggggaaa acgatggggg ttatgctgtc agcagttacc gaaaggtaaa
      781 tccaaaactc ggcacgatga aacagctcgc ccgactagcg caggagctcc gtgagaatgg
      841 catcagcctt gtgcttgacc tggtattcaa ccatacatcc aatgagcata catgggcatt
      901 gcaggcaaga gaagggagtt tggattatca ggattattat cgcattttcc cagatcggat
      961 cgtgccggat gcatatgaaa aacatttgcg tgagatattc cccgatgaac atgccggtgc
     1021 atttacattc tggcccgaga tgaagacatg ggtgtggact acctttcact cttaccaatg
     1081 ggacttgaat tatgcaaatc cagttgtttt caaccgcatg gttgaggaga tgcttttcct
     1141 ggcaaatcaa ggggtagaag tgctgcgtct agatgccgtt gccttcattt ggaaggagtt
     1201 gggaacgaat tgcgagagct tacccaaagc ccatatgctg atacaggctt tcaacgctgc
     1261 ggcgcgtatt gctgctccag cccttttgtt taaatcagaa gctatcgttc acccggatga
     1321 agttatccaa tatatctcac ccgacgaatg ccagctatca tataatcctt tactcatggc
     1381 cttgttgtgg gaagccatgg ctacccgtaa ggtgggtttg ctgtcccaag cgctgcgcga
     1441 ccgcttcgca attcctacac actgtgcctg ggtcaattat gtgcgctgtc atgatgacat
     1501 agggtggaca ttttcagacg aagatgctga ccgtttgggc ataaatggtt attcccaccg
     1561 aaaatttttg aacgattttt ataccggacg cttccctgga agctttgcca gagggttgcc
     1621 ctttcaggaa aatcctaaga caggcgatgc gcgcatttca gggacctgtg cttcgttagc
     1681 ggggttagag aaagcggtgc gcgaggaaac tgatgttgaa gtcgagcttg ccatccgtcg
     1741 tatcttgctc attcacggag taatccttac tgtaggtggt atccctctga tctacttggg
     1801 agacgagatt ggcagtttga atgattacag cttccgtgac gatcctggca aggcagcaga
     1861 tagccgatgg gtgcatcgaa tgagagtcga ctgggaaaaa tatgcccgac gttcaaattc
     1921 tgctacgctc gagggacggg tttaccaggg gatctgccgc ttgattaatt tgcgtttgag
     1981 taggcctgta tttgctggtc aggatatgca agtgctcgaa acggataacg agaatgtttt
     2041 aggctattta cgtaggcacg ataacagtat cgctctcatt tttgctaact tcagtgagcg
     2101 tgaacaaaca gtttccgcca accttcttcg tctctatggt ctgagttaca acactcagga
     2161 tatgttttca ggggcaaagc ttccacaagg ggatataaat ttgaaaccat acgatttcct
     2221 cgctctggct ccacaataag tttgagggca tctattgccc caaatcagat aacgtcagta
     2281 gttgatgaat tagatcgtac atggtggtac agaaattcat ccgagcaaaa ctcactgcgc
     2341 aaattgggca atcgcatttg ggcgatttcc caccactgag gagagaaaat gaggtgtttt
     2401 gatgggggta tgcccccacc aaaacacccc aaacactctt tttacgggat tgcgcatgcc
     2461 cctcagggca tatgcaattg ccctgctgcg caaagatggt gatatgaacc ctatcgttta
     2521 taatgggaac atatctccat ggcgctctta agggcttacc ttttccgttc tatttgggtt
     2581 gtaactgagg atgggcgcac tctcgatttg ggttcgccta ctgtgcgttc attgttcgcg
     2641 tatcttctgt tacaccgcga ccatcccagc gacaggaggc ggctggcatt tttgttctgg
     2701 ccacgagcca gcgaagccgc agcccgtcga aacttgcgtc aatatcttca tcggttgcgg
     2761 cgtgcactgg agccagttga tccactaggt gggttgttaa ttgccgagca ctccggtgta
     2821 cagctggatc caagtgccca gatatgggtg gatgtagagg cgtttcggca caatacgcgc
     2881 cctgaagcta cacttcccga tttacaaacc gcagcggagt tgtactctgg cgatcttctc
     2941 gaagatgtat atgaagact
//
LOCUS       DBNGSX010000192         2208 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_707560, whole genome shotgun sequence.
ACCESSION   DBNGSX010000192 DBNGSX010000000
VERSION     DBNGSX010000192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_707560"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /locus_tag="AC16CB_06000"
     CDS             <1..1418
                     /locus_tag="AC16CB_06000"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HIR3342055.1"
                     /translation="KEQALIRLEVGSERPTSYWVYALVSPFPTLEQGVSSDELRNTWD
                     GNPAGPFAGKPILLDESTLAVLTALWGKPADGAVQVMAAGELTGYAWDRSPWWAIIPF
                     ESLGPRWKVLEVDGISPLRKEFDPASYGLAVPVSLNGDAALAEAILSMYAAGSSAALV
                     PTGNRDPNKLTVLAMTGVTALVRATAYTMEQRGIKYPAKDIGDWLRDADITHVSNEVP
                     FAKNCPFPDPVQQGMRFCSDPRYIALMEDVGTDIVELTGDHFQDWGEEAMYYTLDLYK
                     ERGWLYYGGGEDLQEGRQAVTIEHNGNRLAFIGCNGKGGSFAQAARNHPGAVSCNFEY
                     MEAEIARLRAEGYLPVATFQHFEYYTYRAQPDQVRDFRRLAGAGAVIVSGSQAHQPQA
                     FEFEAGALIHYGLGNLFFDQLDVSQATRQAFIDRHVFYDGRYIGTELLTMLFVDYARA
                     RPMTSGEREELLRAVFNASGW"
     gene            1506..2060
                     /gene="dcd"
                     /locus_tag="AC16CB_06005"
     CDS             1506..2060
                     /gene="dcd"
                     /locus_tag="AC16CB_06005"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HIR3342056.1"
                     /translation="MGLKPDHWIRKMAREQRMIEPFADSQVRDGVISFGVSSYGYDIR
                     VADEFKIFTNVFSAVVDPKQFDPRSMVDYKGEVCIIPPNSFALAKTIEYFRIPRKVLT
                     VCLGKSTYARCGIIVNVTPFEPEWEGYVTLEISNTTPLPAKIYANEGIAQVLFFEADD
                     MCEISYADKKGKYQGQQSIVLPKL"
     gene            2069..>2208
                     /locus_tag="AC16CB_06010"
     CDS             2069..>2208
                     /locus_tag="AC16CB_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020490804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR3342057.1"
                     /translation="MGFANRVLNLQAEGAYAVLARAQELEAQGREILHLEIGQPDFQT
                     FP"
BASE COUNT          474 a          622 c          665 g          447 t
ORIGIN      
        1 tgaaagagca ggcgctcatc cgcctggagg taggcagcga gaggccgacc agctactggg
       61 tttacgcctt ggtatcgccc tttcccaccc tcgagcaagg tgtatcatcc gatgagctac
      121 gcaatacttg ggatggaaac cctgctggtc cgttcgctgg caaacccatc ttgttagacg
      181 agagcacgct ggcggtcttg acagccctgt ggggcaaacc agccgatggt gcggtccagg
      241 tgatggctgc cggggagttg acgggctacg cctgggatcg cagcccgtgg tgggcgatca
      301 tcccatttga gagccttggg ccgcgctgga aagtgctgga ggtagatggc atctcccctc
      361 tgcgaaaaga gtttgacccg gcgagttatg ggctggctgt gcccgtttcg ctgaacggcg
      421 atgcagctct agcggaggcg attctctcga tgtacgcagc gggatcaagc gctgcgctgg
      481 tcccgacagg caaccgcgat ccaaacaagc tgaccgtgct ggccatgacg ggcgtgactg
      541 ccctggtgcg cgccacggca tataccatgg agcaacgcgg catcaagtat cccgccaaag
      601 atattggtga ctggctgcgg gacgcggaca tcacccatgt cagcaacgaa gtgcctttcg
      661 ccaaaaattg ccccttccca gacccggtgc agcagggcat gcgtttttgc agcgacccgc
      721 gttatattgc cctgatggaa gacgtgggca ccgacatcgt ggagctgacc ggcgaccact
      781 ttcaagattg gggcgaagaa gcgatgtact ataccctcga tctatacaag gagcgaggct
      841 ggctgtacta tggtggcggg gaagacctgc aggaggggcg ccaggcagtg accatcgagc
      901 acaacggcaa tcggctggcc ttcatcggct gcaacggcaa agggggcagc tttgcccagg
      961 cggcacggaa tcaccccggg gcggtgtcct gcaatttcga atacatggag gcggagatcg
     1021 cccgcctgcg agcggagggc tacctgcccg tcgccacctt ccagcacttt gagtattaca
     1081 cctaccgtgc ccagcccgac caggtacgcg acttccgccg cctggcgggt gccggggctg
     1141 tcattgtcag cggcagccag gcgcaccagc cgcaagcgtt tgagttcgag gccggggcgc
     1201 tgatccatta cggcttgggc aacctgtttt tcgaccagct cgatgtcagc caggccactc
     1261 gccaggcgtt tatcgaccgc cacgtgttct atgacgggcg ctatatcggc acagaattac
     1321 tgaccatgct gtttgtggac tacgcccgtg cccgcccgat gacctctggg gagcgggaag
     1381 agctgctgag agccgtgttt aatgcaagcg gatggtaatc aaataaatat gcaattcatg
     1441 ggtttagtca tgcttaacag acaacccatt gagctgataa aacttattgt aaaaaggaga
     1501 tatttatggg actaaaacca gatcactgga tacgcaagat ggcgcgtgag cagcgcatga
     1561 tcgagccgtt cgccgacagc caggtgcgcg atggggtgat ttccttcggc gtctcgtcct
     1621 acggttacga tatccgtgtg gctgacgagt ttaaaatctt taccaatgtg ttttcagcgg
     1681 tggtcgatcc caagcagttc gacccgcgct cgatggtgga ctacaagggc gaggtgtgca
     1741 tcatccctcc caactcattt gcgctggcta aaaccatcga gtatttccgc atcccgagaa
     1801 aagtcttaac cgtgtgtctt ggaaaaagca cctacgcccg ctgcgggatt atcgtcaacg
     1861 tgacaccttt cgagcccgag tgggagggct acgtcacgct ggagatctcc aacaccaccc
     1921 cgctgcctgc caagatctac gccaacgaag gtatcgccca ggtgctgttt ttcgaggccg
     1981 acgatatgtg cgaaatctct tatgccgata agaaaggcaa gtaccagggg cagcaatcca
     2041 tcgtcttgcc gaagttataa tatatagaat gggattcgcc aatcgagtgt tgaacctcca
     2101 ggccgagggg gcatacgctg tgctggcccg cgcccaggag ctggaggcgc aggggcgcga
     2161 aatcctgcac ctggagatcg gccagcccga tttccagacc ttcccgca
//
LOCUS       DBNGSX010000193         3375 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_500371, whole genome shotgun sequence.
ACCESSION   DBNGSX010000193 DBNGSX010000000
VERSION     DBNGSX010000193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_500371"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..517
                     /locus_tag="AC16CB_06015"
     CDS             137..517
                     /locus_tag="AC16CB_06015"
                     /inference="COORDINATES: protein motif:HMM:NF024860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HIR3342058.1"
                     /translation="MRLHPQVHCNYQAHFFTRQPLLEALVADEEVGMWLSRRSNRWNQ
                     GRDLSPLVLRAAADFIMERDARRAGKGNPGCIVGDKSPNSLLDGDAVRQLVKVYPDAR
                     LVFIVRDGRDAAVSHRFQAFINRS"
     gene            638..988
                     /locus_tag="AC16CB_06020"
     CDS             638..988
                     /locus_tag="AC16CB_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015016 - heparan sulfate
                     N-sulfotransferase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HIR3342059.1"
                     /translation="MHNVVETDKAARELLPESYHSLRYEDLLTSTWETMRTLWITLGA
                     DITSPGLKEAVDAELQENPDADWQQEKASDIASALTKGQRGTWRELFTPHDRQIFEEI
                     AGGTLAEWGYEVKS"
     gene            985..1974
                     /locus_tag="AC16CB_06025"
     CDS             985..1974
                     /locus_tag="AC16CB_06025"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3342060.1"
                     /translation="MKFLIAGLGSIGRRHLRNLLQLGERDILLYRTGRSSLPDDELES
                     YPVETDLEAALDHQPDAVIVANPTSLHLEVAIPAAERGCHLLLEKPISHSMDSVDQLQ
                     KAVQRGGGQVLVGFQFRFHPGLRKIKELLSASTSPIGKPLSARAHWGEYLPDWHPWED
                     YRQGYSARAELGGGVILTLCHPLDYLRWLLGNVEALWAFAGRLGNLSLDVEDTAEIGL
                     RFADNTLGSVHLDYNQRPSAHTLEIVGSQGTIRWDNADGAVHLHHEKWHVFPVPPSFE
                     RNDLFLAEMRHFQAVVRGEEEPLCSLDDGLQALRLALAARQSALECKLIKLDH"
     gene            1986..3149
                     /locus_tag="AC16CB_06030"
     CDS             1986..3149
                     /locus_tag="AC16CB_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011290458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylyltransferase domain-containing protein"
                     /protein_id="HIR3342061.1"
                     /translation="MDCLAIIPARGGSKGIPRKNILPLAGKPLIAYNIEQAQRSRYIS
                     RLVVSTDDAEIADVARQYGAEVVWRPTEISGDTASSESALLHVLESLRQDEGYLPEVL
                     VFLQCTSPLTLAEDIDGTVQALLDQDADSALAVIPFHYFLWRQAEKDATGINHDKRIR
                     PLRQEREPQYLETGAVYVMRVEGFMRARHRFFGKTAMYIMPAERRLEIDDPVDFQVAE
                     VLLHQEARGAKSARLPEQVAALILDFDGVFTDDKVYVFQDGREAVRCDRFDGMGIGLL
                     KKTGLPIWVLSSESNPVVQARADKLGIPCLHGLQDKGLALGQLLHEQGIDPAQVVYVG
                     NDVNDLPCMAQVGYAVAVADAHPAVLAQADLVLEHSGGDGAIREICELILDNK"
     gene            3159..>3375
                     /locus_tag="AC16CB_06035"
     CDS             3159..>3375
                     /locus_tag="AC16CB_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HIR3342062.1"
                     /translation="MAKAVKIGDRWVGEGYPVFIIAEIGINHNGNLHIAKRLVDVAFM
                     AGCDGVKFQKRTPEKAVPPEFQNVMRET"
BASE COUNT          688 a         1014 c         1010 g          663 t
ORIGIN      
        1 gcccgcgccc ggttcgcgcc gccaggtgcc cgccatcacc cctgaggagg ttgccgaagc
       61 caaaagcttc ttccttctgg agaagttctt tatcttcggt cacgcccgct caggcaccac
      121 cctactcaca cgcttagtgc gactgcaccc ccaggtgcac tgcaactacc aggcgcactt
      181 cttcacccgc cagccactgc tcgaagccct ggtggctgac gaggaagtag gcatgtggct
      241 ctcgcggcgc agcaaccgct ggaaccaggg ccgcgacctc tcgccactgg tgctgcgcgc
      301 tgccgccgac ttcatcatgg agcgcgacgc ccgccgtgca ggcaagggca accctggctg
      361 cattgtgggc gacaagagcc ccaacagcct gctggatggc gacgcagtgc gccagctggt
      421 caaggtctac cccgacgccc ggctggtctt cattgtgcgc gatgggcgcg atgccgccgt
      481 ctcgcatcgc ttccaggcat tcatcaatcg ttcctaacac ctgaacaccg aagacctgcg
      541 catccgtgac gaattcatcc gcgatgccac gcctttcgtc tccgggcaac gctcgatttt
      601 caccgaaaaa agccttgcgc aagccgcccg cggctgggtg cacaatgttg tcgagacgga
      661 caaagcggcg cgggagctgc tgccagagag ctatcactcc ctgcgctacg aggacctgtt
      721 gacttctacg tgggagacca tgcgcacatt gtggatcacc ctcggggcgg acatcacaag
      781 cccagggctg aaagaggccg tggacgccga gctgcaggaa aaccccgacg cagactggca
      841 gcaagagaaa gccagcgaca tcgcctcagc actgaccaaa ggccagcgcg gcacctggcg
      901 tgagctcttc accccgcatg accggcagat cttcgaggaa atcgctgggg gaacgttggc
      961 agagtggggg tacgaagtca aatcatgaaa ttcctgattg cgggcttagg ctccatcggt
     1021 cgccgccatc tgagaaacct gcttcaacta ggggagcggg atatactgct ctaccgcact
     1081 ggtcgcagca gcctgccgga tgacgagttg gaaagttacc cggtggagac tgacctggag
     1141 gctgccctgg atcatcaacc agacgcagtg atcgtcgcca atcccacatc gctgcacctg
     1201 gaggtagcta tccctgccgc cgagcggggc tgtcacctgc tgctggagaa gccgatatcg
     1261 cattctatgg acagcgtaga tcagctacaa aaagctgtcc agcgcggtgg cgggcaagtc
     1321 ctggtgggct tccagttccg cttccaccct ggattgagaa agataaagga actgctctcc
     1381 gcctccacat ctccgattgg aaagccgctc tctgcccgtg cccactgggg ggagtacctg
     1441 cccgactggc acccttggga ggattaccgt cagggatata gcgctcgcgc cgaattaggc
     1501 ggcggtgtga tcctgaccct gtgccacccc ctggactatc tgcgctggct tttgggaaac
     1561 gtcgaggcgc tgtgggcttt tgccgggcgc ctgggcaacc tgtcgctaga cgtggaagac
     1621 actgctgaaa ttggcttacg atttgctgac aatacgttgg gaagcgtgca tttggattat
     1681 aatcaacgac cgtctgccca cacgctggag atcgtgggca gccagggcac gatccgctgg
     1741 gacaacgccg acggagccgt ccacctgcat cacgagaaat ggcatgtctt ccccgtcccg
     1801 cccagctttg agcgcaacga tctgttcctg gcagagatgc gccacttcca agcggtcgta
     1861 cggggtgaag aagagccgct ttgcagcctg gacgacggct tacaggcttt gcgcctggca
     1921 ttggctgctc gccaatctgc cctagagtgc aaactgatca aactggatca ttgaccgagt
     1981 aagagatgga ctgtctcgct attatccctg cccgcggcgg ctctaagggc atcccgcgca
     2041 agaacatcct tcccctggct ggcaaaccgt tgatcgccta caacattgaa caggcgcagc
     2101 gttctcgcta catcagccgg ttggtggtct ccaccgacga cgctgagatt gccgacgtgg
     2161 cgcgccagta tggggcggag gtcgtctggc ggcccaccga gatcagtggc gataccgcct
     2221 catcggaatc ggctctgttg cacgttttag agagcctgcg ccaggacgag ggatacctgc
     2281 cggaagtcct ggttttctta cagtgcacat ccccccttac cctggctgaa gatatagatg
     2341 gcaccgttca agccttgttg gatcaagacg cagacagcgc cctggcggtg atccccttcc
     2401 attattttct ttggcggcag gccgagaaag acgctacagg catcaaccac gataagcgaa
     2461 tccgtcccct gcgccaggag cgcgagccac agtacctgga gaccggggcg gtttacgtga
     2521 tgcgcgttga gggtttcatg cgggcgcgtc accgcttctt cggcaagacc gccatgtaca
     2581 tcatgcccgc cgagcgccgg ctcgagatcg atgacccggt ggattttcaa gtcgccgagg
     2641 tgctgctgca tcaggaagcg cgaggcgcca aaagcgcacg cctgccggag caagttgccg
     2701 ccctgatcct ggatttcgac ggcgtattca ccgatgacaa ggtttacgtt tttcaagatg
     2761 ggcgcgaggc cgtgcgctgc gaccgtttcg atggtatggg cattggcttg ctcaagaaga
     2821 ccgggttgcc catttgggtg ctctccagcg agtctaaccc tgtggtgcag gcccgtgccg
     2881 ataagctggg tatcccttgc ctgcatgggc tgcaggacaa aggtctggcc ttgggacaac
     2941 tcctccatga acagggcatc gaccccgccc aggtggttta tgtgggcaat gatgtcaacg
     3001 atctgccctg catggcgcag gtcgggtatg ctgtggcagt agccgacgcc catccggctg
     3061 tgctggctca agctgacctg gtcctggagc acagcggcgg cgatggggcg atccgagaga
     3121 tttgtgagtt aattctagat aataagtaag gagactgcat ggcaaaagca gtaaaaattg
     3181 gcgatcgatg ggttggtgag ggatatcccg tgttcatcat cgcagagatt ggcatcaacc
     3241 acaacggcaa tctacacatt gccaagcgcc tggtcgatgt ggcttttatg gctggctgcg
     3301 acggggtgaa attccagaag cgcacacctg agaaggctgt accacccgaa ttccagaatg
     3361 tgatgcgcga gacgc
//
LOCUS       DBNGSX010000194         8417 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1400128, whole genome shotgun sequence.
ACCESSION   DBNGSX010000194 DBNGSX010000000
VERSION     DBNGSX010000194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8417)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8417)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8417
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1400128"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="AC16CB_06040"
     CDS             complement(<1..466)
                     /locus_tag="AC16CB_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011228693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR3342063.1"
                     /translation="MQITLKVFRFNPETDKKPHYEKYSIEGQSTDRALDLLEHVKGYI
                     DGTLSYRRSCAHGICGSDAMRINGRNRLACKALVKDVGSKITVEPILGLRVIKDMIVD
                     MEPFFKSYRSIMPYLVNDDPVPNQERLQSPEQRERFDDTTKCILCAACTTSCP"
     gene            complement(481..2271)
                     /locus_tag="AC16CB_06045"
     CDS             complement(481..2271)
                     /locus_tag="AC16CB_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005313121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3342064.1"
                     /translation="MKTHQFDVVVVGAGGAGLMAALYASKTASTAVLSKLYPTRSHTG
                     TAQGGVGAALGNEEEDHPEWHTYDTVKGSDYLGDQDAIEFMCTEAVQAVYEMEHMGLP
                     FSRTSEGRIAQRPFGGHTNNVTGKPVRRACYAADRTGHMILQTLYQQCIKNKVNFFDE
                     FQVVDLILSNNAAAGVVAIELLTGEFHVFHAKSIIFATGGHGRIWELTSNAYAYTGDG
                     IAITMRRGIPAEDMEFFQFHPTGIYKLGILITEGVRGEGGVLINDQGERFMETYAPTV
                     KDLASRDVISRAMYLEMKEGRGIGGKRYLYLDVRPETVNKYAAKDGRKRPDGSPYSVT
                     GDEILRKLPDIIDFCRTYLGVDPVQQPMPVQPTAHYTMGGIPTNKYGEAVIDEKNAVL
                     PGLFAAGEAACVSVHGANRLGTNSLLDLLVFGKHSGLRAAEYAKGASFQSLPQDATEF
                     ARAQFDAILNNGGSEKAADIALEMKSVMNDHVSVFRTEEGMQTALEKVRELKERFKHV
                     QVTDRGKIFNTELLTTWEIGNLIDLAEVTTVCALARKESRGAHARDDYPKRDDGNWMK
                     HTLAWTRDDGIRLSYKPVVITKFQPKERVY"
     gene            complement(2296..2715)
                     /locus_tag="AC16CB_06050"
     CDS             complement(2296..2715)
                     /locus_tag="AC16CB_06050"
                     /inference="COORDINATES: protein motif:HMM:NF013307.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342065.1"
                     /translation="MHARSVQPGVSFEYMMWIFTRISGLAMILLAIIGMSGAFILGAR
                     TQVDLPTLMRWSFFPNPNHVVNSNIPDVAVGWATAYWQIMQMMIFFFASTHAFNGLRV
                     VLEDFIGQSFLQPLLRGAIFLLWLFSLITAVFVILAS"
     gene            complement(2737..3126)
                     /locus_tag="AC16CB_06055"
     CDS             complement(2737..3126)
                     /locus_tag="AC16CB_06055"
                     /inference="COORDINATES: protein motif:HMM:NF013307.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     transmembrane subunit"
                     /protein_id="HIR3342066.1"
                     /translation="MAINRKVGFFQGLSYHGGGPMLSWMLHRVTGLGILAFVGLHVIS
                     SFFMQQTGSDIAAFINTIYESWVFQIFVAFFVLFHALNGLRIAVLDIWPKMLNYEREA
                     LWLQWFIFVPVYGLTVYLLIQRSLVGG"
     gene            3400..3885
                     /locus_tag="AC16CB_06060"
     CDS             3400..3885
                     /locus_tag="AC16CB_06060"
                     /EC_number="7.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="QcrA and Rieske domain-containing protein"
                     /protein_id="HIR3342067.1"
                     /translation="MTNSPHLDRRQFVNVVLTFLSAIIGAVLGLPAIGYLISPATKVQ
                     KKDEWITLGPLEKYQVGTPTLFSFNRTSINGWEKTVNSYGAYVVRFSPDNIKVFSNMC
                     THLSCRVNWKEDLKEYICPCHDGRFDYEGKITAGPPPQEMHEYETKIEDGNLFIHFLE
                     S"
     gene            3888..4547
                     /locus_tag="AC16CB_06065"
     CDS             3888..4547
                     /locus_tag="AC16CB_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012263.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - quinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HIR3342068.1"
                     /translation="MAAKIFAWIDERMGITSIYNLVLDRAVPKVNWWYTLGSASLFLF
                     ALQGVTGIFLAVYYVPSPDHAYESIQYIMNNVAFGWLIRGIHHWGASLMVLFVFIHML
                     RTFYYAAYKFPREITWLTGVVLFLATLGMGFTGYLLPWNQRAYWATTVGTEIAGSAPL
                     IGDFILRVLRGGNDLSAVTLARFFAVHIWFLPAIIALIIGVHLYLVIRLGISALPKEE
                     D"
     gene            4547..5740
                     /locus_tag="AC16CB_06070"
     CDS             4547..5740
                     /locus_tag="AC16CB_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3342069.1"
                     /translation="MDEQQKESYLKKYKQDKSQGVKFYPDIIYKDLLASFAIFVLLVG
                     LAIFVGVKPEPPADPSDSAYIPRPEWYFLFLFELLKYFPGQLEWIGTVILPTIAILAL
                     FLLPFYDRSPFRHWKKRKLAVGVMGTIVLAIVALTVIAAVSTPPQEETNLATSLSESI
                     LLGGDLYSVHCVECHGADGEGGEIKGVEGLEGVVLKPINSQDEMYTRSDDTLYTIIDY
                     GQPDLGMPPFGVGYGGELRRGDTQAVVNFMRYTWDDRAEIPEDVTQASAIPTLAPNQV
                     PSYVEHIQPIFKRYCISCHRPGKKNNNYIMRTYEEVITTGDHAPNIISGNLESNTLLM
                     LDRQEIEAGGPMPPTKALKPELIDIISRWIVAGAPNTPEEAAALTVPADTAIPEGTLP
                     ATTTP"
     gene            complement(5928..6311)
                     /locus_tag="AC16CB_06075"
     CDS             complement(5928..6311)
                     /locus_tag="AC16CB_06075"
                     /inference="COORDINATES: protein motif:HMM:NF013307.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     transmembrane subunit"
                     /protein_id="HIR3342070.1"
                     /translation="MTQLAEQIKTTTGNQAEVVAWKWMRYSGLLLIPLAWGHVAIQDV
                     LVGVHSIDLDYVAMRWASLAWRTYDFLLLAFAFAHGVNGLRQVIHDYIHNEKLKRKLN
                     WILIVFWLVVSAIGAIAIVGGVRLG"
     gene            complement(6439..6843)
                     /gene="sdhC"
                     /locus_tag="AC16CB_06080"
     CDS             complement(6439..6843)
                     /gene="sdhC"
                     /locus_tag="AC16CB_06080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HIR3342071.1"
                     /translation="MDSVRTTINGFASYRMRTGQLSFILHRLTGLGTLLFLAIHILDT
                     STVYFFPELYQHAIELYRSTPMMIGEIILVFSVIYHGVNGARIAIFDLFSPKWWQSGP
                     QERTALVTLVVAILLWLPAAFLMARSLIIHNF"
     gene            complement(7111..8415)
                     /gene="icd"
                     /locus_tag="AC16CB_06085"
     CDS             complement(7111..8415)
                     /gene="icd"
                     /locus_tag="AC16CB_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HIR3342072.1"
                     /translation="MSSNTIKAPVGGETITIRDGLLQVPDHPIIPFVEGDGTGRDIWR
                     ASQRVFDAAVQKAYRGRRKIHWMEVYAGQKAFDKFGSWLPEETVEAFREYLVGIKGPL
                     TTPIGGGIRSLNVALRKALDLYVCQRPVRYFNGVPSPVKHPEYVDMVVFRENTEDIYT
                     GIEFEDGGEDNQRFKRLLKEHFPEDYAKIRFPDTAGIGLKPISKEGTARLVRAAIRWA
                     LENSRRSVTLVHKGNIMKFTEGAFRNWGYEIAEKEFGERVYTWAQWERTKDAQGEDVA
                     NVEQEAAVKSGQLLVKDVIADIVFQQTITRAREFDVIATMNLNGDYLSDALAAQVGGI
                     GIAPGGNINYTTGHAVFEATHGTAPKYADKDMVNPGSVILSGEMMFNYMGWREAAQFI
                     VKGMEKAITAKTVTYDFHRLMEGATLLKCSEYGDAVIKHMQD"
BASE COUNT         2072 a         2329 c         2077 g         1939 t
ORIGIN      
        1 aagggcagga ggtagtgcag gctgcgcaca gaatgcactt ggttgtatcg tcgaagcgct
       61 cgcgctgctc agggctctgc aagcgttcct gattgggtac tgggtcatcg ttgaccagat
      121 agggcataat ggagcgatag ctcttgaaga aaggctccat atctacaatc atgtccttga
      181 tcacgcgcaa tcccaagatg ggctcgacgg tgatcttact gcccacatct ttgaccagtg
      241 ctttacacgc caggcggtta cgtccattga tgcgcatggc gtcggagcca caaatgccgt
      301 gcgcgcagga gcgtcgataa gagagcgtgc cgtcaatgta acccttgaca tgctccagca
      361 aatctagggc ccggtcggtg gactgtccct caatagagta tttttcgtaa tggggcttct
      421 tatcagtctc tggattgaag cgaaagactt tcagtgtaat ctgcatgctc gcccgccttt
      481 ctagtaaacc cgttcttttg gctggaattt tgtgatcacc accggtttgt aacttaaccg
      541 gatcccatca tcccgagtcc aggcgagggt gtgtttcatc cagttcccgt catcgcgctt
      601 gggatagtca tctcgggcat gcgcgccgcg gctctccttg cgcgccagcg cacacaccgt
      661 ggttacctct gccaggtcaa tcaagtttcc tatctcccaa gttgtcaaca actcggtatt
      721 aaatatctta ccacgatcgg ttacctggac gtgcttaaag cgctctttca gctcgcggac
      781 cttttccagg gcggtctgca tgccctcttc ggtgcgaaaa acactcacat gatcgttcat
      841 caccgatttc atctctagag caatatcggc ggctttttcg gaacccccgt tgttcaatat
      901 ggcatcaaac tgggcacgcg caaactcggt agcgtcctgg ggtaagctct ggaagctggc
      961 tcccttggca tattcggctg ccctcaagcc agaatgctta ccaaagacca gcagatcaag
     1021 cagcgagttg gttcccaagc gatttgcgcc gtgcactgag acgcaggctg cctcgccagc
     1081 ggcgaacagg ccaggcagaa cagcattttt ctcgtcaatg actgcctcgc cgtacttatt
     1141 ggtagggatg ccgcccatgg tgtagtgggc agtaggctgc acaggcatgg gttgctgcac
     1201 tgggtccacg cccaggtaag tgcggcaaaa atcgatgata tccggcagct tgcgcagaat
     1261 ctcgtcacca gttaccgagt aaggtgaacc atctgggcgc ttgcggccat ctttagctgc
     1321 gtatttattc accgtctccg ggcgaacgtc caagtagaga tagcgcttac cgccgatgcc
     1381 gcgcccctct ttcatctcga gatacatggc gcggctgatt acatcacgcg aagcgaggtc
     1441 ttttaccgtg ggcgcgtagg tttccatgaa gcgttcaccc tgatcgttga tcagtacccc
     1501 gccctcgccg cgcacgccct cggtaatcag aatgcccagc ttgtagatac cagtggggtg
     1561 gaattgaaaa aactccatat cctcagcagg gatgccgcgg cgcatcgtaa tcgcgatgcc
     1621 atcaccagtg taggcatagg cgttcgaggt tagctcccaa atgcgaccat gaccaccagt
     1681 agcaaagatg atcgacttgg cgtggaagac atggaactca cctgtaagca actcaatcgc
     1741 caccacgcca gccgcagcgt tattggatag aatcaggtca accacctgaa attcgtcgaa
     1801 aaaattgacc ttattcttga tgcactgctg gtaaagcgtt tgaaggatca tatgaccggt
     1861 gcgatcagct gcgtagcagg cgcgccgcac tggcttgcca gtaacgttgt ttgtgtgtcc
     1921 cccaaagggt cgctgggcga ttcgcccctc agacgtacgc gagaagggca agcccatgtg
     1981 ttccatttcg tacacggcct gcaccgcctc ggtgcacatg aactcaatcg catcctgatc
     2041 gcccaggtaa tcactacctt taacagtatc gtaagtatgc cattcaggat gatcttcttc
     2101 ctcgttgcct aaagctgctc ccacgccccc ctgagctgtg cccgtatgag aacgggtggg
     2161 atacagctta ctcagcacag cggtgctggc agttttggaa gcgtacaagg ccgccatcag
     2221 acctgctccc cccgcgccga cgacgacaac atcaaattgg tgggtcttca ttaaatgtct
     2281 tcttctcctt cagtctcagg atgccaagat cacaaataca gccgtaatga gcgagaacaa
     2341 ccaaagcaag aagatcgcgc ctcgcagcag aggttgcagg aacgactgac ctatgaaatc
     2401 ttcaaggaca acgcgcagtc cattaaaagc gtgcgtagat gcgaagaaga atatcatcat
     2461 ctgcataatt tgccaatagg cagtcgccca acccacggct acatcgggga tgttgctgtt
     2521 taccacgtgg tttgggttag ggaagaatga ccaacgcatc aaggtaggca aatcgacctg
     2581 cgtgcgcgcc cccaggataa aagcgccgct catcccaatg atcgccagca agatcatcgc
     2641 cagccctgaa atgcgggtga agatccacat catgtattca aagctcacgc cgggttgtac
     2701 acttcgagca tgcatattac acctcctggc tagattttag ccgcccacca gcgaacgctg
     2761 gataagcaag tacactgtca aaccgtagac cggcacgaag atgaaccact gcaaccacaa
     2821 ggcttcccgt tcgtagttta gcatcttcgg ccagatatcc aaaacagcga tgcgcagccc
     2881 gttcaaggcg tggaacagca caaagaatgc cacaaagatc tggaataccc aggattcgta
     2941 aatcgtgtta atgaacgcag cgatatcgct tccggtttgc tgcatgaaaa acgaggaaat
     3001 aacatgcagg ccaacaaacg ccaggatgcc tagcccggta acgcggtgca acatccagga
     3061 aagcatgggt cccccgccat gatacgaaag cccctgaaaa aagccgactt tccgattgat
     3121 agccatagcg cctcctatca ctcatcttct atgatgttca gaatctacac agtagggagt
     3181 taatgtttgg agcgcaactt ccccgcaatg tttcttcaac tgcgtaaatc cgactttaat
     3241 agtatatcta ttattgctat acgagtgcac aagtaatagt gacaattatc acagatattt
     3301 agtttcatgt gtcctttgaa cttatcctta ttttatctaa aatagaaagg tatttgaaca
     3361 tattaatcgc acaaatcatc gagcaaaagg ggaattcgca tgactaactc accccatctt
     3421 gatcgccgcc agttcgtcaa tgtcgttttg acattcctga gcgccatcat tggggctgtg
     3481 ctgggattgc cagcaatagg ctacctgatc tcgccggcta ccaaagtcca aaagaaagac
     3541 gagtggatca ccctcgggcc tctagagaaa tatcaagtgg gcactccaac tctattcagc
     3601 ttcaaccgca cctccataaa tgggtgggaa aagaccgtca acagttatgg ggcatatgtg
     3661 gttcggttca gcccagataa cataaaagta ttttccaata tgtgcacgca tctgagctgc
     3721 cgggttaact ggaaagagga ccttaaagaa tatatttgcc catgccacga tggacgcttc
     3781 gactatgagg gtaagatcac ggcaggccca ccgccgcaag aaatgcacga atacgagacc
     3841 aagatagagg atggcaacct gttcatccat ttcctagaga gttagccatg gctgcaaaaa
     3901 tcttcgcctg gatcgacgaa cgtatgggta taacgtcgat ctacaatctc gttctggacc
     3961 gtgctgtgcc caaggtgaac tggtggtaca ccttaggcag cgcctcgctc ttcctgttcg
     4021 ccctacaagg agtaaccggc atcttcctgg cagtatatta cgtgccatcg cccgaccatg
     4081 cctacgagag tatccagtac atcatgaaca atgttgcgtt cggttggctg atccgaggca
     4141 tccaccattg gggtgccagc ctgatggtgc tctttgtgtt tatccatatg ctgcgcacat
     4201 tttactatgc agcctacaag ttcccccgag agatcacctg gctgaccggc gtcgtgctgt
     4261 ttctggcgac cttgggcatg ggcttcactg gatacctgct accctggaac cagcgggctt
     4321 actgggccac cacagtcggc acagaaatcg ctggctcagc accattaatc ggagatttta
     4381 tcctgcgcgt cctgcgcggg ggcaacgacc taagcgccgt taccctggct cgcttcttcg
     4441 ctgtccatat ctggttcctg ccagcaatca ttgccctaat tatcggcgtt cacctttacc
     4501 tggtcattcg gctaggtatc tcggcgttgc caaaagaaga agattaatgg acgagcaaca
     4561 gaaggaaagc tatctaaaaa agtacaagca agataagagc cagggggtta agttctaccc
     4621 cgacatcatt tataaagacc ttttggcatc ctttgctatc ttcgtgctgc tggttgggct
     4681 ggcgattttc gtcggtgtga agcctgagcc accggctgat ccgagcgatt cagcctacat
     4741 cccaagaccc gaatggtact ttcttttcct attcgagctt ctcaagtact tcccggggca
     4801 actggaatgg atcggcacag ttatcctgcc aaccatcgcg attctggcgc tatttctgct
     4861 acccttttac gatcgctccc cattccgcca ctggaagaag cgcaagttgg cggtcggtgt
     4921 gatgggcacc atcgtcctcg ccatcgtagc actaaccgtc atagccgctg tctctacgcc
     4981 acctcaagag gaaaccaatc ttgcaacctc cctaagtgag agcatcctgc tgggaggaga
     5041 cttgtattct gtccactgcg ttgagtgcca tggagcggat ggcgagggcg gagaaatcaa
     5101 gggggtggaa ggactggaag gggtagtgct caagcccatc aatagccagg acgagatgta
     5161 tacccgctcc gacgataccc tgtacaccat cattgattat ggccagcctg acctgggcat
     5221 gccgcctttc ggcgttggtt acggtgggga actacgccgc ggcgatacac aggctgtggt
     5281 aaacttcatg cgctacacct gggacgaccg cgccgagatt ccagaggacg tgactcaagc
     5341 tagtgccatc cccaccctgg ctccaaacca ggtgccgtct tatgtcgaac atattcaacc
     5401 catcttcaag cgctactgca tctcttgtca tcgacctgga aaaaagaaca ataattacat
     5461 aatgcgcacg tacgaggagg tcatcaccac gggcgaccat gcccctaata tcatctcagg
     5521 taatttagag agtaacacgc tgttgatgtt agatcgccag gagatcgagg cgggcggtcc
     5581 aatgccgcct accaaagcac tcaagcccga gctgatcgac atcatctcgc gctggattgt
     5641 tgctggagcg cctaacacgc ccgaagaagc agcggcgctc accgtgccag cagacacagc
     5701 aatacccgag ggaaccctgc cagctactac aacaccatag cttcatcact gagccaatgc
     5761 caaaatcatc gccattccat cgccgtaaac actgctgatc catcacagcc gacaacacta
     5821 ccgcaccgcc gaacatcgca tcaatttttc agcaggaagc gagggatccc tcggattgca
     5881 aagcccccca agtatggggg gcttttctta tcaatcagaa agatttatta ccctaaccgg
     5941 acaccgccaa caatggcaat tgcaccgatc gccgacacca ccagccaaaa gacgatcaga
     6001 atccaattga gctttctctt caatttttcg ttgtgaatgt agtcatggat cacctggcgc
     6061 agaccattca cgccatgggc aaaggcaaaa gccagcagca agaaatcgta ggtgcgccaa
     6121 gccagcgaag cccagcgcat agcgacataa tccagatcga tgctatgcac acccaccagt
     6181 acatcttgga tcgcgacatg tccccaagcc aaagggatga gcagcaaacc gctgtagcgc
     6241 atccacttcc aggcgacaac ttcagcctga tttccggttg ttgttttgat ttgttctgct
     6301 agttgagtca ttttctgctt agattcctaa aaaataaaaa atcattcctc atcaaggtga
     6361 tactcagtcg ggctacactg gatggacctt agcgatcatt cttagactaa aatgcgccat
     6421 cacccaacta cctgagcact aaaaattatg aatgatcagg ctgcgggcca ttagaaatgc
     6481 cgctggtaac cacagcaaga tggcgaccac cagggtaacc agggcagttc tctcctgtgg
     6541 tccagattgc caccacttcg gcgaaaacag gtcaaagata gcgatgcgcg ctccgttaac
     6601 tccatggtag atcacactga aaaccaggat gatctcgcca atcatcatcg gggtggagcg
     6661 gtacagttcg atcgcatgtt ggtacagctc cggaaagaaa tatactgttg acgtatccag
     6721 gatgtggatg gccaggaaaa gcagcgtacc caggccggtt aagcgatgaa ggatgaaact
     6781 taactgaccg gtacgcatgc gataactggc aaatccgttg attgtggttc gaaccgaatc
     6841 catgagggag acgctcctgt tctaaaagat ataaggaatg ctaatatact gttgttttga
     6901 ttcgaaatat tttactatgt aatcattaaa cccatttaag atcagaacgg gaaccaatta
     6961 attgaaggcc aattccaatt aagaggaaga aatccctggc gatgcgctcg acgatctcct
     7021 gctggcagcc attgatctgc ctgaggggcg ggacaccaag ctggctctga tccgcttcgc
     7081 ggccaagcct atgccatcaa agtagattaa ctaatcctgc atgtgtttaa tcaccgcgtc
     7141 accgtactcg gagcacttga gcagtgtggc gccttccatc aggcggtgaa agtcatatgt
     7201 caccgtctta gcagtgatag ccttctccat acctttgaca ataaactggg ccgcctcacg
     7261 ccaacccatg taattaaaca tcatttcgcc ggatagtatg acggaaccgg ggttgaccat
     7321 gtccttgtca gcgtacttgg gcgctgtgcc gtgggtagcc tcgaatacag catgcccggt
     7381 ggtatagttg atgttccccc cgggcgcgat gccaatgcct cccacctggg ctgccagcgc
     7441 gtcggatagg taatccccgt tcaggttcat tgtggcgatc acgtcaaact cgcgcgcccg
     7501 ggtgatggtc tgctggaata cgatatcggc gatcacatcc ttgaccaaca gctggccaga
     7561 ttttacggca gcctcctgct cgacgttagc aacgtcttcc ccctgggcgt ctttggtacg
     7621 ctcccactgc gcccaggtgt aaacccgctc gccgaactcc ttctccgcaa tttcatatcc
     7681 ccagttgcgg aaagcacctt ccgtgaattt catgatgtta cccttgtgta ccagggtcac
     7741 gctgcgacgc gaattctcta aagcccagcg gatggccgct cgtaccaagc gagccgtccc
     7801 ctctttggag atcggcttca ggccgatccc ggctgtatca gggaagcgga ttttggcgta
     7861 atcttccgga aaatgctctt tcaataatcg cttgaagcgc tggttatctt cccctccatc
     7921 ttcaaactcg atgcccgtgt agatgtcttc ggtgttctcg cggaagacaa ccatgtccac
     7981 gtattccgga tgcttgaccg gggaaggcac gccattgaaa tagcgcacgg gtcgctggca
     8041 aacgtacagg tcaagggctt tacgcagggc aacgttcaga gaacggatgc caccaccgat
     8101 gggtgtggtc aaggggcctt tgatgcccac caaatactcg cgaaaggcct ccacggtttc
     8161 ttcaggcagc caagaaccga atttatcgaa ggctttctga ccagcgtaga cctccatcca
     8221 atggatcttg cgcctgcctc gataggcttt ctgtacggca gcatcaaaca cgcgctgcga
     8281 cgcccgccag atatcgcgtc cggtgccatc tccttcgacg aaggggataa tgggatgatc
     8341 cggcacctgc agcaacccat ctcggatggt aattgtctct ccgcccactg gcgcttttat
     8401 tgtattggaa ctcatga
//
LOCUS       DBNGSX010000195         3305 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_916081, whole genome shotgun sequence.
ACCESSION   DBNGSX010000195 DBNGSX010000000
VERSION     DBNGSX010000195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_916081"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..852
                     /locus_tag="AC16CB_06090"
     CDS             178..852
                     /locus_tag="AC16CB_06090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3342073.1"
                     /translation="MSNFMKRICSKVWENGLTRTFQQFISVMEDYYFDLKHGIDTSEI
                     VQVEDLDITREDKKHSRRYQPTRIRYIKKLIRELKLPEDSVFVDFGSGKGRVLFVASN
                     YNFKRVVGVEISSKLCEIARNNLVVYERKLKRPLQVEIVESNVLRYDIRDDENIFYFF
                     RPFDGLIMEKIIEKIISSLKESPRKVWVIICSSIYDDLFEGKQIFQRSMGYAYGGAEF
                     VVYVNH"
     gene            complement(1108..1734)
                     /locus_tag="AC16CB_06095"
     CDS             complement(1108..1734)
                     /locus_tag="AC16CB_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342074.1"
                     /translation="MGNIRIEKLAPLSGVISLLLFCIGAALLGIYEYLPIADKLKAIL
                     DTNASSIFTGGYLGSISAFFMIWFAGSVYSALREQEGGTGRLSMVAFGGGVASGVALA
                     SGFSAILTSGARAGAAGGITAVEAVTLYDLYSQVLGGMFAITMAVFIGATGTVSLRTA
                     MFPSWFGWVSVLIAIGLLTPFAYAVLALVLIWLIIVSFWLYRRGLSTT"
     gene            complement(1865..2359)
                     /locus_tag="AC16CB_06100"
     CDS             complement(1865..2359)
                     /locus_tag="AC16CB_06100"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HIR3342075.1"
                     /translation="MLTQSMQNAFNEQIKNELYSAYLYLSMSSHFEASNLPGFAKWMR
                     LQSEEELEHALKFYDYVNERGGRVTLFEIPQPKSDWKTSLEVFNQVLEHEQHVTSLIH
                     KLYETALSEKDYASQVMLQWFIDEQVEEEKNATEIVEQLKLIDVHGTAVLMLDHRLSK
                     RGKE"
     gene            2600..>3305
                     /locus_tag="AC16CB_06105"
     CDS             2600..>3305
                     /locus_tag="AC16CB_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342076.1"
                     /translation="MNPPVARRFNMPFVVRNVRLQRGLAFGVIIIAALLSFEIFNYST
                     TDFALSDLLGELEFFGIRWATILAIAFCGIDFAGIARLFTPEVGDDEPTEVWYLFGAW
                     LLAATMNAMLTWWGVSIALLQHESLGNAVISREQLLRVVPIFVAVMVWLIRVLIIGTF
                     SVAGDRLFSQADKRVRGQSPFSLRNTRVRQVPQNAHISAKSAQAFNPAPKPSPGDVVY
                     SRPEPTYHPVSMQARSA"
BASE COUNT          951 a          621 c          777 g          956 t
ORIGIN      
        1 agtaaagaat ttgagccatt gggatcttct aaattcccaa gactgcgctt tgtgcgtagg
       61 caataacatt ttcaaagatc gagaagggtt cccatatcga tctaccaaaa ccagaggtga
      121 tggtgatagt aagtagtatt gatttcgcga gatgaacgtg cttttgaggt attacaaatg
      181 tcgaatttta tgaagcgtat ctgtagcaag gtttgggaaa atgggttaac aagaactttt
      241 cagcaattta ttagcgttat ggaagattat tacttcgatt tgaagcatgg aattgataca
      301 tcagagatcg ttcaggtgga agatttagat ataacacgtg aagataaaaa acatagtcga
      361 cgatatcaac ctactcgcat cagatatatt aaaaaattga tcagagaatt aaaattgccg
      421 gaggatagtg tatttgttga ttttggatct ggtaagggga gggttttatt tgttgcatca
      481 aattacaatt ttaagcgtgt tgtgggtgtc gagatatcca gtaaattatg tgaaatagcc
      541 agaaataatc ttgtggtgta cgagaggaag ttgaagagac ctttacaggt ggaaatcgta
      601 gaatcaaatg ttttacgata tgacattcgt gatgatgaaa acatttttta tttctttagg
      661 ccattcgatg gactaattat ggaaaaaatc atcgagaaaa taataagctc tttgaaagaa
      721 agtccaagaa aagtgtgggt aattatttgt tcttctattt atgatgattt gtttgaaggg
      781 aagcagatat tccagagatc tatgggttat gcgtatggag gggcggaatt tgtcgtttat
      841 gtaaaccatt gaagttgtat cttgaaagtg agaaaataga ttgccaaaaa ttagtggtcc
      901 aaataattgg tccaccgtag ccttgatagt ttacacagat gttgcaagac gggtgattgc
      961 aggaattaaa ctcaaacgga aagatctgtc taatccagca tcaagctgac ggggcttcgc
     1021 tcaagtttaa gtggtgcgaa ggattgtcct ttggtggtat agaaattgag tttcgggcaa
     1081 aactcgcttc gcagcttggg cgaatcttta tgttgttgat agaccgcgcc gatacaacca
     1141 gaagctaacg atgatcaacc aaatcagtac aagagcaaga acggcatatg caaaaggggt
     1201 gagaagaccg atggcaatca acacgctcac ccagccaaac caactgggga acatggctgt
     1261 gcgaagtgag acggtccctg tggctccgat aaagactgcc atagtgatag caaacatgcc
     1321 tcctaagact tggctataaa gatcatacag ggtaactgct tcgactgcgg tgatgccacc
     1381 tgcggcacca gctctcgccc cggaggtcaa gattgcggaa aatccagatg ccagggcaac
     1441 accagatgca acaccacctc caaatgccac catagacaag cgtcccgtac caccttcttg
     1501 ttcgcgtaat gcgctgtata cgctgcctgc gaaccaaatc atgaagaatg ctgagattga
     1561 gccaaggtag ccaccggtaa aaatgctgga agcgttggtg tcgaggatgg ccttaagttt
     1621 atcggctatt ggtagatatt cgtagatacc gagcagtgcc gccccaatgc aaaacagcaa
     1681 aagggatatg acacctgaga ggggtgctag tttttcaatt cgaatattgc ccacaattca
     1741 cctccaaagt tgtggtcgct tgttacctac taataaacta atagatacaa cccccttagg
     1801 ttcatatggt tttgttgaaa tttgaagaaa aaagccgctt acatactcat tgatacggtt
     1861 tggtttattc tttgccgcgc ttactgagac gatgatccag catcaggacg gctgttccat
     1921 ggacatcgat gagcttaagt tgttctacaa tctcggtggc gttcttttct tcctcaactt
     1981 gttcgtctat gaaccactgt aacatcacct gggacgcgta atctttttca gaaagagctg
     2041 tttcgtagag cttgtgaatc aatgatgtaa cgtgctgttc atgttctaaa acctggttaa
     2101 aaacttccag tgatgtcttc caatcagatt tgggttgagg gatttcgaaa agtgtaacac
     2161 gcccgccgcg ttcattgacg tagtcgtaaa acttgagtgc atgttcaagt tcttcttcag
     2221 attgaagtct catccattta gcaaaaccag gaagattgct ggcttcgaag tgagaactca
     2281 tggacaaata gagataggct gaatacagct catttttgat ctgttcattg aaggcatttt
     2341 gcatggattg ggtaagcata tgttctcctt gtattgaaga atttttagtt gggtagattg
     2401 tttatcacgt atttggagtg tcacgcttga ttcgctgaag agaaaatttt atatgtgctt
     2461 atagtttaat ataaaaattg cctcttgaca aaatagaact tatgttctaa aataggtcaa
     2521 cttgcattgc aatgaatcat agaaatgtac aggtacctga aagtataaat cttaaattga
     2581 tcaggatagg aggtttccaa tgaacccacc ggtcgcaaga agatttaata tgccattcgt
     2641 tgttcgcaat gttcgattgc aacgtggttt agcatttggg gtcatcatca tcgcagcctt
     2701 gttgtctttc gagatattta attacagcac tacagatttt gctctctccg atttactggg
     2761 agaactagaa ttctttggaa ttcgctgggc taccatttta gcgattgcct tttgtggtat
     2821 cgattttgct ggtatcgcac ggctatttac cccagaggta ggagatgatg agcccaccga
     2881 ggtctggtat ttgtttggag catggctttt ggctgctacg atgaatgcta tgctgacctg
     2941 gtggggtgta tctatcgcgc tcttgcaaca tgagtcgctc ggaaatgcag tgatttccag
     3001 agaacagttg ctgcgtgtcg taccgatctt cgttgccgtg atggtttggt tgatccgtgt
     3061 gctgatcatt gggacattct cggttgcagg cgatcgattg ttcagccaag ctgataagcg
     3121 tgtaagaggg caatctccat tctcattgcg caatacaagg gtgagacaag taccccagaa
     3181 tgcgcatata tctgcgaaat cagctcaagc tttcaaccca gcaccaaaac caagtccagg
     3241 agatgttgtc tactctcgcc ctgaaccaac ctaccatcca gtctcaatgc aggctagatc
     3301 agcaa
//
LOCUS       DBNGSX010000196         6427 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1400274, whole genome shotgun sequence.
ACCESSION   DBNGSX010000196 DBNGSX010000000
VERSION     DBNGSX010000196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6427)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6427)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6427
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1400274"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="AC16CB_06110"
     CDS             complement(<1..223)
                     /locus_tag="AC16CB_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342077.1"
                     /translation="MKKRQLMFFVLIAILALSVTTATFAKAPRHFIVDLQPLNDSGVW
                     GTAHLLLDGDQLTVMIEALGLEADRPHAQH"
     gene            complement(770..1690)
                     /locus_tag="AC16CB_06115"
     CDS             complement(770..1690)
                     /locus_tag="AC16CB_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019603795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HIR3342078.1"
                     /translation="MKIEEERNRFRGCLLGLAVGDAVGTTVEFRPRGSFALLTDMVGG
                     GPFHLLPGQWTDDTSMALCLATSLVECNGFDARDQMERYNQWARAGYLSSTGSCFDIG
                     NTVNSALIRFRQTGEPFAGSTDPRSAGNGSIMRLAPIPMFFFPDLDEVERYAALSSRT
                     THGAAESVDACRLFARVIYRALLGRSREEVLFGDSESFDGCKKILAIARGLYREKPKT
                     EIRGSGYVVQSLEAALWCFVHTDNFKDTILMAANLGDDADTTAAVCGQIAGAYYGENG
                     IPPTWLEKLTMRDEIAELAESLYRGHAFLP"
     gene            complement(1665..3488)
                     /gene="pepF"
                     /locus_tag="AC16CB_06120"
     CDS             complement(1665..3488)
                     /gene="pepF"
                     /locus_tag="AC16CB_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HIR3342079.1"
                     /translation="MSEPNVLPRSEIPEKYTWNSTSVFSTKEAWEAALKELPELTARV
                     TKYQNHLGDNPSMLVEALQAFEKLYRQSGMVYVYATMSQAVDTTNQEANRIRSRAQSA
                     FAQVFAALAFIDPELISIGQDKLRKWIEDEPRLRSYGHYLENLFRRQAHVRSPEVENL
                     LGMLSDPMGNIYNTANMLANADIQFSPAYTSDGGEVQVSNSTYDSILSSPDREARRTA
                     YESYTDSYLSLKNTFASNLITSVKSNIFMMRGRRHPSTLEASLFESNIPTSVFHNLID
                     TYRKNLPTWHRYWAIRRKALGVDELQPYDIWAPLTQKHPQLTYEETVEWICRGLAPMG
                     KDYVDTIRRGCLEDRWVDVFPNQGKMDGAFSSGWPGTFPFILMSFDGTIFSLSTLAHE
                     LGHSMHSYLTFEHQPVIYSDYSLFLAEVASNFHQAMVRAYLLNNSDDPDLQISVIEEA
                     MSNFHRYFFIMPTLARFELEIHQREERGEGISADDMNGLMADLFAEGYGDEMSYDRQR
                     VGITWATFSHLYTDYYVFQYATGISGANALSRRILDQKSGAVEDYLGFLKAGGSQYPL
                     DILKRAGVDLTTPEPVEETYAILAGLIDRLERLIENRGRTE"
     gene            complement(3609..5984)
                     /locus_tag="AC16CB_06125"
     CDS             complement(3609..5984)
                     /locus_tag="AC16CB_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3342080.1"
                     /translation="MAKITHKLNNSLEGALAGGGDPATSPLYVFGPFLRMIVVAGVAQ
                     ITFGASIWLVVLTIAVVSAMYRLVMLWVTDGSGGSGLTEEEFGSWAAKINAAITFVEY
                     TLTFLVSMAAMVTFIADRIPILNNKFLFLQYRTLIAILLSFLTGWLVNRGPKVAARTF
                     GPATAGVLLLLWAMVISSIFKYGLRLPTLSLQAFSLQNLSYTIRGYASILAVMTGIEV
                     FANLVAAYEGSPGEKSNRAFNSLVIIMGTTIVTMLVVGPAILAISDPTDPSVSVFTQT
                     MDVLLPWPLPWLGSLVGIAVLMSASAASAQGLQNLALGLKRRHYIPPIIGKQNTFEVA
                     EYPVWIEVSIVSLCFLLFGTHEETYLAIYAAGVFILLSMTGWGVAKRLIRQFRTDFSV
                     EHSLMIIGISITAVLTTGATLIIFYERFLEGAWTYFLFIPALYGIFSYFRDRLGEPTA
                     AMDYLGQIDSTRLAGFGFGQSAVPTIREILSPQPEQIVWHPAPKEASWWKQEKIVFRR
                     VAVLLDGSANAAQALPMAKRVCQATDAEMILLSSVKAVGDPDYENIFNERKAYLRQQC
                     YELEQQGYRVSYTVRPGHIADATSALVKEQYIDLIVTSTSGKSGTKHWQRGGVSRKLM
                     ALLNIPVLLVKVSEMDILMPSFKRIVLSLDGSEFSERALPYASVFAELFDSELILLSV
                     PEVPEVKDYRAAAGVIDTIREKAYANMHEYLNETANSLLQTNGKINVRTMVAGSLPAR
                     TIVNVSEEENADLIMLTSQGRGGLDLLMTGSVALNVVEDTDLPVFMVPIIS"
     gene            6297..>6427
                     /locus_tag="AC16CB_06130"
     CDS             6297..>6427
                     /locus_tag="AC16CB_06130"
                     /inference="COORDINATES: protein motif:HMM:NF024842.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HIR3342081.1"
                     /translation="MSDSIIIIGAGIAGLSAGCYGQMNGYRTQIFELHDIPGGLCTA"
BASE COUNT         1734 a         1611 c         1446 g         1636 t
ORIGIN      
        1 tgtgttgtgc gtgaggtcta tcggcttcca aacctaaagc ttctatcatg acggtcagct
       61 gatctccgtc taacagcagg tgggctgtac cccatacacc tgaatcattc aggggctgga
      121 gatcaacaat aaaatgacgg ggggcttttg caaacgtcgc cgtagttaca ctgagtgcca
      181 gaattgcgat taatacaaag aacataagtt gtcgcttttt catcgggttt tcctttggta
      241 tttctaaaaa gtttacttgt cattatctgg atatatctgc aagatctggg tatctatttt
      301 gttcgcgact aatcctcctt ttgtataatt tagatgtgtt tccaccgaat tacataaaaa
      361 tgtacgtgag ttcgtgccaa ttggatttca tactcaatac attgggtaat tgatgtttcc
      421 aatttcattc caatgcatga atttcccccg caattcaaag gaattgattt cgccccaact
      481 attcagacta cgtggatgct aattacttct ctctaacagg tatttcagag cagattggtc
      541 aaatgccatt aatgaataat ccctgaccga gtcagggatt attcaaacca aaggaggatg
      601 gaagaagcgg caacccacga aggaggttat aaagattata gacaaaaaac cgcaaattgt
      661 tacaatcaat tttgtaagta tcgttttgta gtatcgaacg cagctaacag cttggataga
      721 aatgttaatt ggactgtcct gttaatctca ccaagtgtgg aagtgctaat cacgggagga
      781 aggcatgccc gcgatataaa ctctctgcaa gctcggcaat ctcatcgcgc atggtcaatt
      841 tttccagcca ggtcggtgga atgccgttct cgccgtagta agcaccagca atttgcccac
      901 ataccgctgc agttgtgtcg gcatcatccc ctaagtttgc ggccatgagg attgtatcct
      961 tgaagttgtc ggtgtgaaca aagcaccaca aagctgcttc aaggctttgt actacgtagc
     1021 cggaaccacg gatctctgtt ttcggcttct cacggtatag tccacgggca attgccagga
     1081 tttttttaca tccatcaaac gattcactgt ctccaaacag cacttcttct ctcgatctgc
     1141 ccaatagggc acgatagata acccttgcga aaagacgaca tgcatctaca gactctgcag
     1201 cgccgtgggt cgtgcgggaa ctcaacgctg catatcgctc tacttcatcc aggtccggga
     1261 agaaaaacat ggggatgggt gccaagcgca tgatgctgcc attgccagcc gagcgcggat
     1321 cggttgatcc agcaaatggt tctcctgttt ggcgaaacct aataagagca gagttaactg
     1381 tgtttccgat atcaaagcac gagccagtgc tgctcaagta ccctgcacga gcccattggt
     1441 tgtaacgttc catctgatca cgggcatcga atccattgca ttccacaagg ctggttgcca
     1501 ggcaaagcgc catagatgtg tcatcagtcc attggcctgg caaaaggtga aaagggcctc
     1561 caccaaccat gtccgtcaat agggcaaagc tcccccgcgg tctaaactca accgtggtgc
     1621 caaccgcatc cccgacagca agaccgagaa ggcagcctcg aaatctattc cgttcttcct
     1681 ctattttcaa tcaatcgctc caggcggtcg atcaatcctg caaggattgc atatgtttct
     1741 tcaacaggct caggcgtggt gagatcgaca ccggctctct taagaatgtc caaggggtat
     1801 tgggatccgc cagcttttag gaaaccgaga taatcctcaa ctgcaccaga tttttggtcg
     1861 agtatcctgc gcgagagtgc atttgcgcct gagataccgg tagcatactg gaatacatag
     1921 tagtcggtgt ataaatgtga gaaagtcgcc caggtgatgc ctacccgttg gcggtcgtag
     1981 ctcatctcgt ctccatagcc ttcagcaaat aggtcagcca tgagaccatt catgtcatca
     2041 gccgatatgc cctccccgcg ctcctctctc tggtgtatct ccagttcgaa gcgcgccaat
     2101 gtgggcatta tgaagaaata gcggtggaaa ttactcatgg cttcttcgat tacgctgatc
     2161 tgcaagtcag ggtcgtcgga gttatttaac aaataagctc taaccatcgc ctggtggaag
     2221 ttggatgcta cctctgctag gaagagagaa taatcgctgt aaatgacggg ctggtgctca
     2281 aaagtaaggt aggaatgcat tgaatgccca agctcatgcg ctagcgtgct caggctaaat
     2341 atcgtgccat cgaagctcat caatataaag gggaaagtcc ccggccaacc cgaagaaaat
     2401 gcaccatcca ttttcccctg attagggaaa acgtctaccc atcgatcttc caggcaacct
     2461 ctgcggatgg tatccacgta gtcttttccc atgggcgcca agcctctgca aatccattcg
     2521 accgtttctt catatgttag ctgagggtgc ttttgagtca ggggcgccca gatgtcatat
     2581 ggttgaagct catccacccc caacgctttg cggcggattg cccaatagcg gtgccaggtc
     2641 gggagatttt tgcgataagt atcaatcaga ttgtggaaaa ccgacgttgg tatattgctc
     2701 tcgaagagtg atgcctccag tgtcgaggga tgtcgtcgac cacgcatcat gaaaatattg
     2761 gacttcaccg aggttataag gttgcttgca aaggtgttct ttagtgataa atagctatct
     2821 gtatagctct cgtaggcggt gcgccgcgcc tcccggtctg ggctggacag aatactatca
     2881 tacgtactat tggatacttg cacttctcca ccatcactag tataagccgg ggagaactgt
     2941 atatcagcgt tggcaagcat attggctgta ttgtagatat tacccatagg atcgcttagc
     3001 atgccaagaa gattttccac ttcaggcgaa cgcacatgag cctggcggcg gaaaagattt
     3061 tccaggtagt gaccatagct acgcaggcgc ggctcgtcct cgatccattt ccggagttta
     3121 tcctgcccga tggatatcag ctcaggatca atgaacgcca aagcggcaaa aacttgtgcg
     3181 aaggctgact gagccctgct tcggatccgg ttggcttcct ggttagtggt atccactgcc
     3241 tgagacatcg tggcgtaaac gtaaaccatc ccagattggc gataaagttt ctcgaaagcc
     3301 tgcagcgctt cgaccagcat agatgggttg tcccccaaat ggttctggta ttttgtgaca
     3361 cgcgccgtca actcgggcag ttctttcaat gctgcttccc aagcttcttt tgtcgagaac
     3421 acgctagttg agttccaggt gtatttctca ggtatctcac tgcgaggtaa aacgttaggt
     3481 tcagacattg gtttctccaa gtttagaaca aagattattt atacttcatc tccgtcaata
     3541 cctgactttg tactgctatt agggcaattg ccctaaaaga agcgtttgct cattaaagca
     3601 ttcatcgctc aggagataat tggcaccata aagacaggca ggtcagtatc ctcaacaacg
     3661 tttaacgcca cgctaccagt catcaatagg tctaagcccc cgcgcccttg cgaggtcaac
     3721 ataattaaat cagcgttttc ttcttcgctc acgtttacaa tcgtacgtgc tggcagcgag
     3781 ccggcgacca tggttcgcac attaatcttg ccgtttgtct gcaataagct attggcggtt
     3841 tcgttcaaat attcatgcat attggcataa gctttctctc gaatagtatc aatgacccct
     3901 gctgcagcgc gatagtcctt cacctctggt acttctggta cagacaacaa gatgagctcg
     3961 ctgtcaaata actctgcaaa cacactcgca tatggcagtg cgcgttcaga aaactcggaa
     4021 ccatccagcg agagaacgat tcgcttaaaa gaaggcatta agatatccat ctcagatacc
     4081 ttcaccagta aaacggggat attgagaagc gccattaatt tgcgggaaac cccacccctt
     4141 tgccagtgtt ttgtgccaga tttcccactc gttgaggtga caatcaaatc gatatattgc
     4201 tcttttacca gtgcgctggt cgcatcagca atatgtccag gacgtacagt gtagctaacc
     4261 ctatatcctt gctgctccaa ttcataacat tgttgtctca gataggcttt ccgctcgtta
     4321 aatatatttt cataatcggg atcacccaca gcttttacag aagacagcag gatcatttcc
     4381 gcatcggttg cctggcatac tcttttagcc attggcaggg cttgagcagc gttggctgaa
     4441 ccatccagca agacagcaac ccgccggaat acaattttct cctgtttcca ccaactggct
     4501 tccttgggtg cagggtgcca gacaatctgc tcaggttgag gcgataagat ctcacggatg
     4561 gtaggcacag ccgattgtcc aaatccaaaa cctgccaacc tggtggagtc aatttgcccc
     4621 agataatcca tcgctgccgt cggctctccc aacctgtccc gaaaataact aaaaatgcca
     4681 tataaagcag ggataaacaa gaaatatgtc caagcgcctt ctaaaaaacg ctcataaaaa
     4741 atgattaggg ttgctccagt cgtcagcact gcagtgatgc taatccctat gatcattaga
     4801 ctgtgttcca cagaaaaatc agtccgaaat tgacggataa gtcgtttagc tacaccccac
     4861 ccggtcatgc tgagcaaaat aaaaacgcct gccgcataga tcgccagata agtttcttcg
     4921 tgcgtgccaa ataacaggaa acacaaagaa acaatactca cttcaatcca tacaggatat
     4981 tccgcaactt caaacgtatt ttgcttgcca ataatcggag gaatataatg gcggcgcttt
     5041 aagcctagtg ccaagttctg caatccctgc gcgctggcag ccgatgcaga catcaacacc
     5101 gcaatgccca ccagcgaacc aagccagggc aaaggccacg ggaggagcac atccatcgtt
     5161 tgtgtaaaaa ctgagacgct tggatcagta ggatctgaaa ttgctaaaat cgctggacca
     5221 accaccaaca tggtgacgat cgtcgtaccc ataataatca ccaagctgtt gaaggctcta
     5281 ttagattttt ccccgggact gccttcataa gctgctacca aattggcaaa tacttcaatc
     5341 cctgtcatca ctgccaggat actggcatat ccccgaatgg tgtaggataa gttctgtaaa
     5401 gaaaatgctt gcaggctcaa ggttgggaga cgaagtccat atttgaagat gctcgaaatc
     5461 accattgccc aaaggagtaa cagaacccct gcagttgcag gaccaaacgt gcgcgccgct
     5521 actttagggc cacggtttac caaccagcca gtcaggaaac tgagcaaaat tgcgatgaga
     5581 gtacggtact gtaagaacag gaatttatta tttaaaatgg gtatccggtc tgcgataaaa
     5641 gtgaccatgg cagccatgct gaccagaaaa gtaagtgtat actcaacaaa agtaattgcg
     5701 gcatttatct tagccgccca gcttccaaat tcttcctcgg ttaaaccact tcctccactt
     5761 ccatcagtca cccaaagcat caccaagcgg tacatggcgg aaaccactgc aatggtgagc
     5821 accaccaacc aaatagaggc tccaaatgtg atttgggcta ctccagcgac cacaatcatg
     5881 cgcaggaaag ggccaaaaac gtataacggt gaagttgccg ggtcgccgcc tccggccagc
     5941 gctccctcca aagaattatt aagcttgtga gttattttag ccattgatga tttcccgatc
     6001 cataattgtc tccagtgtac agatgaatta aattcaacgc aacacagcgt gcatctaatt
     6061 ttacccaaat atcgctccct tccgatagtc acattcttct aaatcgggta aacccactct
     6121 ccaggtgaac aacgggattt atccattcga aaaggtcatc gataggcagg atactgcaca
     6181 aacccctccc cgtacaagtt tttttgcttt actcctgcca agcttaaaag caacgtagta
     6241 caataatcct gccgacataa acaatcatct acacgcatag tatgggaggg tctaatatgt
     6301 ctgattcgat cattatcatt ggggcgggaa ttgccggtct atccgccggg tgctacggtc
     6361 agatgaacgg ttaccgcacg cagatcttcg aactgcacga tatacccgga ggattatgca
     6421 ccgcctg
//
LOCUS       DBNGSX010000197         3134 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_569356, whole genome shotgun sequence.
ACCESSION   DBNGSX010000197 DBNGSX010000000
VERSION     DBNGSX010000197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_569356"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="AC16CB_06135"
     CDS             complement(<1..386)
                     /locus_tag="AC16CB_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3342082.1"
                     /translation="MADEKTPLVQVQHLKKYFPIFRGLIIQRRIGSIKAVDDVTFDIY
                     KGETLGLVGETGSGKTTVGRTLLRLYEPTDGRVIFDGNDIFRLNENEMRNMRVRMQMI
                     FQDPYASLNPRMTVGSIVAAPLDVHK"
     gene            complement(386..1378)
                     /locus_tag="AC16CB_06140"
     CDS             complement(386..1378)
                     /locus_tag="AC16CB_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3342083.1"
                     /translation="MDYILEIKGLQTRFYTDEGVVHAVNGVSYGMEQGEILGVVGESG
                     CGKSVHALSILRLIPDPPGKIESGEIWFDGRDMLKMSENEMRYIRGKQIAMVFQDPMT
                     SLNPVYTVGFQIMEALTYHLSMDYKQARDRAIELLTIVGIPEASQRVDDYPHQFSGGM
                     RQRAMIAMALSCIPRLLIADEPTTALDVTIQAQIVDLVKRLQAELGMAVMWITHDLGV
                     VAEIAQRVNVMYAGYIIERGKVRDIFKHPRHPYTQGLLGSLPRLDEAPGTKLVSIPGL
                     PPDLILLPKGCPFAARCVFVQDRCREEHPPLEEAGESEHIVACWRWEEIAKGEA"
     gene            complement(1391..2620)
                     /locus_tag="AC16CB_06145"
     CDS             complement(1391..2620)
                     /locus_tag="AC16CB_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3342084.1"
                     /translation="MAEITPQEIGITDFALREHRSLWYDAWRRLISSNTARLGLFIVA
                     IFVLSSVYAHYFWKYDPKTDLDYSLKLKAPNLAPTQEIESVHLFGTDKLGRDIFRRVL
                     HGGWNSLRVGIVAVGISLSVGGLLGVLAGFFESTMISSRENILVRIMIGVVLSLLPAW
                     IANQYFQVVFFGVFGAIAAIYDRFLAGKSARFLIFALVGAVISGVTGLMVSPYIAFMC
                     ALIGALIGAIVSLPFGGQFLSSTIMRVMDIILSFPGYLLAIAIVAFLGPGLEKGMIAI
                     GFVGIPVYARLARSVVLSVMQKEYILAAASVGEGHGRVLSRHVVPNILSPIIVQTTMG
                     LASAILSAAALGFLGLGAIPPEPEWGAMLGDSYRYLTSGAWWAVLFPGLAIILSVLGF
                     NLLGDGLRDALDPKLRI"
     gene            complement(2629..>3134)
                     /locus_tag="AC16CB_06150"
     CDS             complement(2629..>3134)
                     /locus_tag="AC16CB_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3342085.1"
                     /translation="LSTGVEVKSITHLILVDSLLTANIDAFIDALKHIAMPTIALATI
                     PMAIIARMTRSSMLDVLQEDYIRTAHAKGLLERLVLFRHALKNAFLPIVTVIGLQTGT
                     LMAGAVLTETIFAWPGIGKWVYDAILGRDYPIVQGGTLLIAIIFVVFNLLVDVSYAYL
                     DPRIHYD"
BASE COUNT          898 a          851 c          693 g          692 t
ORIGIN      
        1 aatttgtgca catccaatgg cgcagctacg atcgaaccta cagtcattcg aggattgagg
       61 gaagcatagg gatcttgaaa gatcatctgc atgcgcacac gcatatttcg catttcgttc
      121 tcattcagtc taaaaatatc attaccgtca aatatcaccc gcccatccgt aggctcatac
      181 aacctgagaa gtgtccggcc aacagtggtt ttaccacttc ctgtctcgcc caccagacct
      241 aacgtctcgc ctttatagat atcgaatgta acgtcgtcga ccgccttgat agaaccgatc
      301 cggcgctgga tgatcaaccc ccgaaaaata gggaaatact tcttaaggtg ttggacttgc
      361 acaagggggg ttttttcatc tgccattatg cttccccctt agcgatttct tcccagcgcc
      421 aacaagccac aatatgctca ctttcccctg cttcttccaa tggagggtgc tcctcacggc
      481 aacgatcctg gacaaaaaca cagcgagcag caaatgggca tcctttcggc aataagatca
      541 ggtctggcgg caaaccaggg atggatacca gctttgtgcc cggcgcttca tccagacgcg
      601 gcagagaccc gagtaaccct tgcgtgtagg gatgtctcgg atgcttgaag atgtctctta
      661 ctttgccgcg ttcaatgatg taacccgcat acatcacatt aactctttga gcgatctcgg
      721 caacgacccc caaatcatga gtaatccaca tgaccgccat gcccaactcc gcctgtaagc
      781 gtttaacaag atcgacgatt tgggcctgta tggtcacatc caaggcagtg gtgggttcat
      841 cagcgatgag caatctagga atacaagaaa gcgccatggc aatcattgcc cgctgtcgca
      901 tcccgcccga gaactggtgt gggtaatcat ccacccgttg actggcttca ggaatgccga
      961 ctatggtgag cagttcaatt gcacgatcac gagcttgctt gtaatccatg ctcaggtggt
     1021 aggttaacgc ctccatgatc tgaaagccaa ccgtgtaaac cgggttgagc gaggtcatcg
     1081 ggtcctgaaa caccattgcg atctgctttc cacgaatata ccgcatctca ttttcactca
     1141 tttttagcat gtctcggccg tcaaaccaga tttccccgct ctcgattttt ccaggaggat
     1201 cgggtatcag acgcaagatg gacagtgcat gaacgctctt tccgcagccg ctttccccca
     1261 ccacaccaag tatctcccct tgttccatac cgtaagacac accattaaca gcatgcacta
     1321 caccttcatc ggtgtagaaa cgtgtctgca gacccttaat ttctagaata taatccatat
     1381 gaatattatt tcagatgcgc aacttgggat ctaaagcatc acgcaaacca tccccaagca
     1441 agttaaaccc aaggacactt aatataattg ccaaaccagg gaataatacc gcccaccaag
     1501 ctccactggt cagatagcga tagctgtcac ctaacatcgc gccccactca ggctcgggag
     1561 ggatggctcc caatccaaga aatcccagag cagctgctga gaggatagca ctcgccagtc
     1621 ccatagtcgt ctgcacgatg attggagata agatgtttgg tactacatgg cgcgacagaa
     1681 cgcgcccatg cccctctcca accgaagcag ctgccaggat atattccttt tgcataacgg
     1741 agagcacaac ggagcgcgcc aggcgggcgt aaaccgggat gccaacgaaa ccaatggcaa
     1801 tcatgccctt ttccaaccct gggcccaaga aggcgacaat agcgattgcc aataaatagc
     1861 ctgggaaaga caagataata tccatcactc gcatgatcgt gctggatagg aactgccccc
     1921 cgaaaggtaa ggagacaatg gcaccaatca gcgctccaat aagggcacac atgaaagcga
     1981 tataagggct aaccattagc cccgttaccc cagatatgac ggcacctacc agcgcaaata
     2041 ttaagaatct ggccgattta ccagccaaaa accggtcata aatcgcagct atagctccaa
     2101 atacaccaaa aaagactact tggaaatact gattagcgat ccatgctgga agaagactca
     2161 acactactcc gatcataata cgaaccagaa tgttttcacg cgaagagatc atcgtagact
     2221 cgaagaaacc tgccagcaca ccaagcaacc cgccgaccga aagcgagata cctacagcga
     2281 caatgcctac acgcagcgag ttccacccac catgtaaaac ccgccgaaag atatcacgcc
     2341 cgagcttatc ggttccaaat aagtgcactg attcaatctc ttgagtcgga gccagattcg
     2401 gagccttgag cttgagagaa taatccagat cagttttagg atcatatttc caaaaatagt
     2461 gagcatatac gctcgacaga acaaagattg ccacaatgaa caaccctaac ctggctgtgt
     2521 tactagagat caacctgcgc caggcgtcat accacaaaga gcggtgctca cgcagggcaa
     2581 aatccgttat accaatctct tgcggggtaa tctcagccat aatgcacctt aatcgtaatg
     2641 gatcctggga tccagatagg catatgatac atcgactagc aaattaaaga cgacaaatat
     2701 gattgcaata agcaaggttc caccctgaac aatgggataa tctcgcccca gaatagcatc
     2761 gtagacccat ttcccaatcc caggccaggc aaagatggtc tctgtaagga cagccccagc
     2821 catgagagta cctgtttgca aacctataac ggtaacaatt ggcaggaatg cattcttaag
     2881 cgcgtggcgg aataagacca ggcgctctag caacccttta gcatgtgctg tacgaatata
     2941 atcttcttga agcacgtcta gcatgctaga gcgggtcatt cgagcgatga tcgccatggg
     3001 gatggttgcc agggcgatgg tcggcattgc aatatgtttc aaggcatcta taaatgcgtc
     3061 gatattcgca gtaagcagac tgtccaccag gataaggtgt gtaatggatt tgacttcaac
     3121 gccagtgctc aagc
//
LOCUS       DBNGSX010000198         1993 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1054945, whole genome shotgun sequence.
ACCESSION   DBNGSX010000198 DBNGSX010000000
VERSION     DBNGSX010000198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1054945"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(646..718)
                     /locus_tag="AC16CB_06155"
     tRNA            complement(646..718)
                     /locus_tag="AC16CB_06155"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(683..685),aa:Arg,seq:tcg)
     gene            complement(752..>1993)
                     /locus_tag="AC16CB_06160"
     CDS             complement(752..>1993)
                     /locus_tag="AC16CB_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing protein"
                     /protein_id="HIR3342086.1"
                     /translation="ALLFFTGFFLSACAGALASGDNPDLPKETPPTENISPTEQVTSP
                     QKLTVTPTINEQVKPLLPEESPPFGATREFSTDFQKHTVPYAEILSSGPPKDGIPAID
                     NPQLVHIGEADSWLEPLEPVVLIEVGDQARAYPIQILMWHEIVNDTVGELPLTVTFCP
                     LCNTAIAFERKVGDKLLDFGTTGRLRYSNLIMYDRQTETWWQQANGEAIAGELTGTRL
                     KFYPAAMISWQEFKSIHPNGLVLSRETGFNRSYGRNPYTGYDNINNPPFLYDGPETPG
                     VLPPVARVLTVDLNGEAVAYPYDTLQQVQVVNDQVGGEAIAVFWAAGTASALDSSSVA
                     SGRDVGAANAFKRVLLGRELQFAVVDGKIIDQETGSQWNVLGQATSGELAGKKLDPVV
                     AINHFWFSWAAFKPETRIYQP"
BASE COUNT          484 a          496 c          519 g          494 t
ORIGIN      
        1 ttctgaagtg tagtcatgcg caacatatag aacctccttt tggctaggga atttttttat
       61 ctgatgagcg ggttaacaaa ttaggctaga tatggcggct tgagagcgtg tctagtggga
      121 tgggttaccc tttcgttcct ccttcaccac ggacgatcaa tattcgttta acgcgtggca
      181 cgccttgttg ttatcggaag acagctaatg agctatcttg gtggtagctg tggtgaagca
      241 gcgactcctg cgcgtggagt agcggctggc agtgccctaa gccacctagt tgcagcagaa
      301 ggaggcgaac tcgtttattc atcaacaact ttgcgaacag cccgttcagt tacctcccga
      361 tcagagataa aattatcttc agctaggcaa tattgaagga agagtaagtt agagaagcac
      421 atcgccatat aacctgatac aaaatcaggt ctttcccgcc cgacgaagta ccgataagga
      481 tgaatccatc tttaaccaac atgcaagatg gttgatttca atatacgctt tttcatataa
      541 atgtcaatat taggaatatt ttcacaaata aaagaacctg ctaaaaaaaa caggctctta
      601 cccccacaag tgggggatca aaacggcgac aaggctaatt gttagcgccc ccggcacgat
      661 tcgaacgcgc aaccctttga tccgaagtca aatgctctgt ccattgagct acgggggcgt
      721 ctgcctgaaa ttatactcga cagaaggcga ttcagggctg ataaatcctg gtctccggct
      781 tgaaagccgc ccaggagaac cagaagtggt tgatggcgac gactggatcc agtttcttgc
      841 ctgccagctc accgctggtg gcctgcccga gtacattcca ttggctgcca gtctcctggt
      901 cgatgatttt tccatcgacc actgcgaatt gtaattcccg cccaagtaaa acccgtttga
      961 aggcgttcgc tgctccaaca tctctcccgc tggcgacaga actgctatct agggctgagg
     1021 ccgtaccagc tgcccagaaa accgcgatgg cttcaccacc cacctggtcg ttgacaacct
     1081 ggacttgctg aagcgtatca taagggtagg ctactgcctc gccgttcaga tccaccgtca
     1141 gcacgcgcgc cacagggggc aacacgcccg gtgtttctgg gccatcgtac aagaacggcg
     1201 ggttgttgat attatcataa ccggtgtagg ggttgcgacc gtacgaacgg ttgaaaccgg
     1261 tttcgcggga gagcaccaga ccatttggat gaattgattt aaattcctgc catgagatca
     1321 tcgcggcagg gtagaatttc aagcgcgttc cggtcagctc gccggcgatg gcctctccat
     1381 tagcctgctg ccaccacgtt tctgtctggc gatcgtacat gatcagattg ctgtagcgca
     1441 atctgccggt ggtgccgaag tccagcagtt tatccccgac ttttcgttca aaagcgatgg
     1501 cggtgttgca caaggggcaa aaagtgaccg tcaggggaag ttccccaacc gtgtcgttta
     1561 caatttcgtg ccacattaag atttggatag ggtaggcgcg cgcctggtct cccacttcga
     1621 tcaggaccac gggctccagc ggttcaagcc agctatccgc ttccccaatg tggacgagtt
     1681 gcgggttgtc gatggctggt atgccgtcct tcggaggtcc actggaaagg atttcagcat
     1741 agggaaccgt atgcttttga aaatcggtcg aaaactccct cgttgcacca aagggaggcg
     1801 attcttcggg caataatggc ttaacctgct cattaattgt tggtgtgacc gttaattttt
     1861 ggggggatgt aacttgctca gttggggata tattctctgt tggtggagtc tccttgggta
     1921 aatcaggatt atcccctgat gctaatgctc ccgcgcacgc agagaggaag aaaccagtaa
     1981 aaaatagtag agc
//
LOCUS       DBNGSX010000199         5321 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1055039, whole genome shotgun sequence.
ACCESSION   DBNGSX010000199 DBNGSX010000000
VERSION     DBNGSX010000199.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5321)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1055039"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="AC16CB_06165"
     CDS             complement(<1..307)
                     /locus_tag="AC16CB_06165"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3342087.1"
                     /translation="MDKIEMNKFISAIKLTSLPGLFTGIKHQPSTWGKDARAKGIYAA
                     LASALFLGTTPVFGKQAILSGASPLAVVAFRTIFAAILLFIVMLIFNRKYLYIYPAGL
                     "
     gene            complement(300..1973)
                     /locus_tag="AC16CB_06170"
     CDS             complement(300..1973)
                     /locus_tag="AC16CB_06170"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR3342088.1"
                     /translation="MFFAECLDCGHQYHYSPHTVTCPNCNGQWCEGRYDYPALGKIFP
                     SLLKERPFNLWRYRELLPIYEYNQSFSMGEGGTPLVHAANLGMMLGCPNIYIKDERQG
                     PTRSFKDRQAAVGIASLKEAGITEAVIASTGNVAISYSAYAAKAGIKLWAFLTSTVPS
                     EKMREVAIYGTQVVKVTASYDIAKKIAAEFADQRNLCLERGARSIPSVEAMKTIAFEI
                     VEQLTMLFDPKQRPATNQSLPWLVPDWYFQAVSGGLGPLGVLKGFVELKQMGLTDRIP
                     SIANIQVEGCAPMVNAWKKGSDIIEPIHSPRTHISTLATGDPGRTYTLLSQRMQEGGG
                     IFESVSDEETFRTIHILAKLEGISLEPAAAVSFAGLIKAVRAGLIKPNDTVIVNCTGH
                     TMPVETMILGDNWAKDMVLPSFASLATPEEGLLAALEEVTPDRFPRIVIVDDLPDARR
                     LLRRILQSQGDYSIFEASSGQEAIMLAEKENPDLILLDLMMPDHDGFEVLSALKSNNT
                     TANIPVIVITAKELTQGEQERLRGHIHTLMQKGEFLSDELSHEVRALVG"
     gene            complement(2015..>5321)
                     /locus_tag="AC16CB_06175"
     CDS             complement(2015..>5321)
                     /locus_tag="AC16CB_06175"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3342089.1"
                     /translation="YFTDCSPEVESILGIPVKEFLGQPVSEFRLTPESGTRLKSKLEE
                     GAYPIEVAVQYETINGEYIPVVLDILQPPLIEEENGKNRVWHGFARVQLPKTTKQERI
                     PRKTEFFPVMPVPTPRSRSQKTSAQIKPSSRNIGLAKPSTMEIAPQVAKEEVNNLIEL
                     LDASPDRRWSDDELLLIEQVAGQLTLALDNARLFQENLHLLEETSRRNEELTTLNKII
                     STASRSLELFEMLREILIQLLTVLNMETGIICLADMKTRKLSIAVHHNMPNDLLDRLN
                     ADGLDDTLCHQVFEHAKVIKINNLEEALSTDGTNLIENGLMSFIGLPLESRGDILGTA
                     CLFGIAPLSDDTPNLSLLTTVGQQIGVAVENATLFHQTNEVLEETQRQTTNLGVLNEM
                     GRSLTNLSDAESVLENIYKYTSELVDTTNFYIALYDSDAEFVSFPLAMESGQHQNIAS
                     RSMQKFITDHIIRNRNPYLINDDIDTRFQEIGVDDFDLQTPTKSYLGIPLIFGEQVIG
                     MMGLQSPVLNQYTGRELDLLTAVARHAAIALQNVRLLEETRRRAEQLHTAAVIARDST
                     GTLALDLLLDRAANLIYDGFKYYHVSILLLDEKHETAIVHASTGEAGKEMKKSKHSVG
                     VGSKSVIGNVTECGLPLVINDITRDPLFYPNPLLPDTRAEIGIPLKIGQKVVGALNVH
                     ANVVNAFDTDDILVLQILADQIAIAVENARSYELSLNAVDDMRKADQLKTQFLANMSH
                     ELRTPLNSIIGFSRVILKGIDGPITDLQEQDLTAIYNSGQHLLNLINDILDLSKIEAG
                     KMELNFEDNINIPDLIKSVTSTVTGLLKAKPIKLVQEIESALPNVRADPLKIRQVLLN
                     LLSNATKFTEQGSITLRAYTQSGLGEQPQLYISVTDTGPGIAPEDREKLFQPFSQVDA
                     SATRKAGGSGLGLSISRHLVEMHGGQIGVESELGKGSTFYFTLPIPQKNGFFSEDTNP
                     TLTNNPLVLTIDSEMSILQHYERYLMNNGYQAYALTDPLKAIELAIKLHPMAIILDVL
                     IPNFDGWQLLKDLKTDPNTQHIPVIICTLLDNPSKGFSLGADQYLMKPVLEDDMVHAL
                     KRFSNNVSRQNNM"
BASE COUNT         1329 a         1196 c         1305 g         1491 t
ORIGIN      
        1 gtagtcctgc tgggtagata tatagatatt tacgattgaa tataagcatt acgatgaaga
       61 gcaatatggc agcaaaaatc gttcggaacg ccacgactgc taggggagaa gcgccactta
      121 ggatggcttg cttgccaaac actggtgtag ttccgaggaa gagagcagaa gcgagtgctg
      181 cgtaaatccc tttggctcgc gcatccttcc cccaagttga cggctgatgt ttgatgcctg
      241 tgaataaacc tggtaaggaa gtcaatttaa tggcggaaat aaacttattc atttcaattt
      301 tatccaacca gagcgcgcac ttcgtgtgat agttcatcgc ttaagaattc acccttttgc
      361 atcagggtat ggatatgtcc tcgcaggcgc tcttgctcgc cttgggttag ttccttggca
      421 gtaatcacta ttactggtat gtttgctgtg gtgttattgg atttaagcgc gctgagcact
      481 tcaaatccat catgatctgg catcatcagg tccagtagga tcaggtcagg gttttctttt
      541 tcagctaaca taatggcttc ctgcccactg ctagcttcga aaatgctgta atccccctgt
      601 gattgcagaa tacgtcttag caagcgacgg gcatcaggca ggtcgtcaac gatcacaatg
      661 cgcgggaagc gatctggagt aacttcttca agcgcagcta atagaccttc ttcgggagta
      721 gctagtgacg caaaagaagg gagtaccata tcttttgccc agttatctcc caggatcata
      781 gtttccacgg gcatggtgtg cccagtgcaa ttcacaatga cagtatcatt gggcttgatc
      841 aatccagcgc gtaccgcttt gatcaagcca gcaaaagaaa cagccgctgc tggctcaagg
      901 gaaataccct ctagctttgc taaaatgtga atggtacgga atgtctcttc atcagagacg
      961 ctttcaaata ttcctccacc ttcttgcatg cgctgggata ataaagtata ggtgcgtcct
     1021 gggtcacccg tagctaaggt ggatatgtgc gtacggggtg aatgaatagg ctcgatgata
     1081 tctgaacctt tcttccaggc attcaccatt ggcgcacagc cctccacttg aatattggct
     1141 atggagggga tgcgatccgt aagtcccatt tgtttgagtt caacgaagcc tttaaggacc
     1201 cctagcggcc caagtcctcc gctaactgct tgaaaatacc aatctggaac aagccatggc
     1261 agagattggt tggtagcggg tctctgtttg ggatcaaaaa gcatagtgag ctgttcaacg
     1321 atctcgaatg caatggtttt cattgcttcg acagatggga tgctgcgagc gccgcgctct
     1381 aggcaaagat tacgctgatc ggcaaactct gccgcgatct ttttggctat atcgtatgat
     1441 gcggtaacct ttacaacctg cgtaccatag atggcaactt ctcgcatctt ctccgatggg
     1501 acagtgctgg tgagaaatgc ccaaagtttt atccctgcct tagctgcata agcggaatag
     1561 gatatagcta cattgccagt ggatgcaata actgcctcag tgatgccagc ctctttcagt
     1621 gaggctatcc ctacagccgc ctggcgatct ttaaatgatc tcgtaggacc ttgccgctca
     1681 tccttgatat agatattggg acaacccaac atcattccaa gatttgcggc gtgcactaat
     1741 ggggtaccac cttcccccat agaaaatgat tgattgtatt cgtagatggg cagaagctca
     1801 cgatagcgcc acaggttaaa gggacgctcc ttgagtaaag acggaaagat tttgcctagt
     1861 gctggataat cgtagcgccc ctcgcaccac tgaccgttac aatttgggca tgtgaccgta
     1921 tgaggtgaat agtggtactg gtggccacag tcgagacatt cagcgaaaaa catggacgat
     1981 ggcggagact gtgtggtgtg attcaaagtt ggctttacat gttgttctga cgtgatacgt
     2041 tgtttgagaa ccgcttgagc gcatgcacca tatcatcctc taaaacaggt ttcattaggt
     2101 attggtccgc gcccaggctg aaaccttttg atggattatc taatagggtg catatgatga
     2161 cagggatgtg ctgagtattc gggtcggtct tcaaatcctt cagtagttgc catccgtcaa
     2221 aattggggat caagacatcc aggatgatag ccattggatg gagcttaatt gccaactcaa
     2281 tagccttcaa gggatctgtc agagcgtagg cttggtagcc attgttcatg agataacgct
     2341 cgtaatgctg caggatggac atttcactgt cgatcgtaag aaccagaggg ttgttggtaa
     2401 gggttggatt tgtgtcttcc gaaaaaaatc catttttctg cggaattggc agagtgaaat
     2461 agaaagtgct ccctttgcct aactcacttt ctacccctat ttgacctcca tgcatttcca
     2521 ccaggtgacg ggagatggat aagcctaatc cgctaccgcc tgctttgcgc gtagccgatg
     2581 cgtccacctg ggaaaaaggc tggaataact tttcgcgatc ttcgggggca atacctggac
     2641 cagtatccgt aacacttata taaagctgag gctgttctcc caatccgctt tgggtataag
     2701 cgcgaagagt gattgaccct tgctcggtaa attttgtggc attcgaaagc aagttgagca
     2761 atacctggcg aatttttagt gggtctgcac gaacattggg cagtgcggat tcgatctctt
     2821 gcacgagctt tatcggtttt gctttgagca gcccagttac tgtggatgtt acacttttga
     2881 tcaggtcggg aatattgata ttatcctcaa aattcaattc catcttgcca gcttcaattt
     2941 ttgacaggtc gagaatatca ttaatcaagt tgagtaagtg ctggccagag ttatagatag
     3001 cggtcaaatc ctgttcctgg aggtctgtga ttgggccatc gatccccttt aatatcacgc
     3061 gcgagaaccc tatgattgag ttaagaggag tacgtagctc gtggctcatg ttggctaaaa
     3121 actgtgtctt cagttgatct gcctttcgca tatcatcaac tgcattaagc gataactcgt
     3181 atgaacgagc gttctccacg gcaatagcga tttgatccgc caggatttga agcaccaaga
     3241 tgtcatcggt gtcaaaggcg tttacaacat tcgcatgcac gtttagagcg ccgactacct
     3301 tttggccgat tttcaatgga ataccgattt cggcgcgtgt atcagggagc aaaggatttg
     3361 ggtagaataa cggatccctg gtgatgtcat tgatcaccaa aggtaagccg cattcggtga
     3421 cattgccgat taccgatttg gagccaacgc ccacagagtg cttgcttttc ttcatctcct
     3481 ttcctgcttc ccccgtggaa gcatgtacga tggcagtctc atgcttctcg tccaaaagca
     3541 agattgaaac gtgataatac ttgaaaccat catagattag gtttgcagcg cgatccagga
     3601 gcaaatccag agcaagtgta cccgtgctat cccgagcaat tacggcggcg gtatgcaact
     3661 gttcggcgcg gcggcgggtc tcttccagca gtcgaacatt ttggagggca atagctgcgt
     3721 ggcgagccac tgcagtcaag agatcgagtt ctcgccctgt atattgattg agcactgggc
     3781 tttgaagccc catcatcccg atcacttgtt cgccaaagat caatgggata cctagatagg
     3841 atttagttgg ggtttgcagg tcgaaatcat caaccccgat ttcctgaaag cgggtatcga
     3901 tgtcgtcatt tatcaaataa ggattgcgat ttcggataat atgatcggtt ataaattttt
     3961 gcatagagcg gctagcgata ttttgatgct gccctgattc cattgcgagt gggaatgaga
     4021 caaattcggc atcgctatcg tacagggcga tataaaaatt ggtagtatcc actagctcgg
     4081 atgtatattt ataaatattc tccaggacag actcggcatc ggataaattg gttagggagc
     4141 gacccatttc gtttagcacg cccaagttgg tagtctgccg ttgggtctct tctaatacct
     4201 cgttcgtttg atggaaaagt gtggcatttt ctacggctac accaatctgc tgaccaacgg
     4261 ttgtcaacaa agataaattg ggcgtgtcat cactcagagg ggcgatgcca aaaaggcatg
     4321 ctgtacccag aatatctcca cgcgactcga gtggaagacc aataaatgac attaatccgt
     4381 tctctataag gttggtacca tcagtcgata aagcctcttc caggttgttg atctttatga
     4441 ctttcgcatg ttcaaatacc tgatggcata atgtgtcatc cagtccatct gcattcaatc
     4501 ggtccaaaag atcattgggc atattgtgat ggacggcaat gctcaatttg cgggttttca
     4561 tatcagccaa gcagattatc ccggtttcca tattcaggac cgtcaagagt tgtattagta
     4621 tttcccggag catctcgaat aactccaggg agcgacttgc ggtgctgatg atcttattta
     4681 gtgtggttag ttcttcgttt cggcgactgg tttcttctag taggtgtaaa ttttcttgaa
     4741 atagcctggc attatccaac gcaagcgtca attgaccagc cacctgctcg atcaataaaa
     4801 gttcatcgtc actccagcga cggtcagggc tcgcgtctag cagctcaatt aaattattaa
     4861 cttcttcttt cgcaacttga ggggctatct ccatagtaga tggcttcgcc aaaccgatat
     4921 tcctgctact tggtttgatc tgcgccgatg ttttttgtga tctggatctc ggcgtgggga
     4981 ccggcattac aggaaaaaac tcggttttgc gcggaattcg ttcttgtttg gttgttttgg
     5041 gtaactgcac cctggcaaat ccatgccaga ctcgattctt gccattttct tcttcaatca
     5101 agggaggttg gagtatgtcc aatacgactg gtatgtattc tccatttatc gtttcgtatt
     5161 ggacagcaac ctcgatagga taagcgcctt cttccagctt ggattttagg cgtgtgcctg
     5221 attcaggtgt caaacggaat tcagaaaccg gttgacccaa aaattcctta acagggatac
     5281 ctagaattga ctcaacctcg gggctgcaat ctgtgaagta g
//
LOCUS       DBNGSX010000200         3158 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_569684, whole genome shotgun sequence.
ACCESSION   DBNGSX010000200 DBNGSX010000000
VERSION     DBNGSX010000200.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_569684"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..385
                     /locus_tag="AC16CB_06180"
     tRNA            313..385
                     /locus_tag="AC16CB_06180"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:346..348,aa:Ala,seq:ggc)
     gene            775..>3158
                     /gene="leuS"
                     /locus_tag="AC16CB_06185"
     CDS             775..>3158
                     /gene="leuS"
                     /locus_tag="AC16CB_06185"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR3342090.1"
                     /translation="MMTELPAYLPAEIEPKWQARWEAEGQYHANVDPSRPKHYALTML
                     PYPSGDLHIGHWYAMTPSDARARYMRMRGYNVMFPMGFDSFGLPAENAAINRGVHPKE
                     WTYANIERMRTQLRSMGAMFDWRREAVSSDPEYYRWSQWFFTQLHKNGLAYRKMSPVD
                     WCPNCNTTLAREQVWGEDRHCERCGTPVIKKDLEQWFFRTTNYAEELLDFSHLDWPER
                     VQLLQTNWIGRSEGAEVHFIVDDPRLSVLESQLTVFTTRPDTLWGATFMVLAPEHPLV
                     AKITTSDQKAEVDAYVQQASRMTDIQRESADKEKTGVFTGAYAINPVNDERIPIWIAD
                     YVLMTYGTGAIMAVPAHDQRDFEFARKFGLEVRVVIQPLDMEVLDGATMVESVPAYGT
                     MVNSGALSGTPGDAAFDAAIHYLEERQVGKRAINYRLRDWLISRQRYWGAPIPIVYCP
                     EHGAVDVSDEDLPMLLPDDVQWLPTGESPLKLHPTWKQTTCPICGQPSERETDTMDTF
                     MCSSWYHLRYLSPHFVRAAFDPDEYAYWMPVDTYTGGIEHATMHLIYTRFFHKACRDM
                     GIVEGPEPMSQLRNQGIVLGEDSEKMSKSRGNVVAPDDLVSRYGADTVRAYLMFFARW
                     NLGGPWNSSGIEGAARWVRRVWIMLTEPAPVANPSSEVLRSLRRKVHQTLQSVTLDFE
                     QFEFNTIISSLMELLNEMSKAREQGAGGTPAWDEAVDIYLRMMAPITPHIAEELWAYM
                     GKPYSIHAQDWPQVDESAASEEEITLVVQVNGKVRDRIVVPVNIGEDEAKAAALA"
BASE COUNT          736 a          772 c          887 g          763 t
ORIGIN      
        1 ccctgacgtt catgcaattc gcacctagat catccctgga cagggcgcag gtcgactttc
       61 cttcctcacg ccgtgtgcaa ccgtgtgcaa cgccggtagc acggcatgga aataatggac
      121 atcttggata cttgtacccc cacaggtggt gatataatat actccttacc acaatatgtg
      181 ggcagtggac actgaggacg acccctcggc atcaatcgca ctgaagaggt cgcgggttcg
      241 aatcccgcca ggtccatttg ttgacgaaac aggtttttgc gattaaaatt ccgtcgctga
      301 gttaacattc cagggcccat agcgcagttg ggagcgcgtc tcaatggcat tgagaaggtc
      361 gagggttcga atccctctgg gtccacagaa aagtctagca ggagtagtag gccatctacc
      421 agcgagccgg gagagcgagg tggaagcccg gcagggtgtt tgtagagcac attttgagtt
      481 gggctgaact cgcctgtcgc ccacgaggca gactccgccg gtgaactaag agtagtcggt
      541 ggcgggtgcg cccgttatcg tgcatcaaag cggtcttcca ctgcttacgg gttatcggat
      601 tgctattcga aatgagcttt attgatctgt aggttgtctt aatggaacag tggaagacaa
      661 gcagggtggt accgcggagc accttcgtcc ctgtattcgg gcgaagggct ttttttattc
      721 gagagtatgg ctaacccgaa tattgaatca aacaggaaat atcagagagt aaaaatgatg
      781 acagaattac ctgcatactt acctgcggaa attgaaccaa aatggcaagc tcgctgggaa
      841 gccgaaggcc aataccatgc caatgttgac ccttcacgcc ccaagcacta tgccttgact
      901 atgctaccct atccttcggg tgatctgcat atcggtcact ggtatgccat gactccttcg
      961 gatgcgcgcg cccgttatat gcgtatgcgt ggctacaatg tcatgttccc aatgggattc
     1021 gattcatttg gtttgcctgc agagaatgca gcaatcaatc gtggagttca tcccaaagaa
     1081 tggacctatg caaatattga gcgcatgcgc actcaattgc gaagcatggg tgcaatgttc
     1141 gattggcgtc gtgaggcggt ttcctcagat ccggaatatt accgttggtc acagtggttc
     1201 ttcacccaat tacataagaa cggtttagcc taccgcaaga tgtcgccggt ggactggtgc
     1261 cccaattgca acacgacatt ggcacgcgag caggtttggg gagaagatcg tcactgcgaa
     1321 cgctgcggta ccccagtgat taagaaagac ctggagcagt ggttctttcg gaccacaaac
     1381 tacgctgaag agcttctgga tttctcccat ttggattggc cagaacgggt gcaattgcta
     1441 cagaccaact ggattggaag gtcggaaggc gcggaagtcc attttattgt cgatgaccct
     1501 cggctatcgg ttctcgaatc gcaactaacc gtttttacca ctcgccccga cacgttgtgg
     1561 ggagctacat ttatggtatt agcgcccgag catccactgg tcgctaaaat caccacctcg
     1621 gatcaaaaag ctgaggtgga cgcatatgta cagcaggcat cgcgcatgac agatatccag
     1681 cgcgagtccg ctgataagga aaaaactggc gtatttaccg gtgcttacgc tatcaatccc
     1741 gtgaatgacg agcgtatccc catctggatt gccgattacg tactcatgac ctatgggaca
     1801 ggtgccatca tggcagtgcc ggcgcatgac cagcgtgact tcgagtttgc tcgtaaattc
     1861 gggttggagg tgcgcgtggt tatccagcct ctggatatgg aggtgttgga tggtgccacc
     1921 atggtagagt ctgttcctgc ctatgggacg atggtcaatt caggtgcttt atcgggtacg
     1981 cccggcgatg cagcttttga cgctgccatt cattatctgg aagaacgtca ggtgggtaag
     2041 cgggcgatca attaccggtt gcgtgattgg ctgatctctc gccagcgcta ttggggggca
     2101 cccatcccca tcgtttattg cccagagcat ggtgcggtag atgtatccga tgaagatttg
     2161 cctatgctgc tgcctgatga tgtccagtgg ttgcccacag gcgaatcgcc gttgaagttg
     2221 caccctacct ggaagcagac tacctgcccc atatgcggcc agccctctga acgcgagact
     2281 gatactatgg acactttcat gtgttcttca tggtatcact tgcgctacct gagcccgcat
     2341 tttgttcggg ctgcctttga tcctgatgaa tatgcctatt ggatgccagt agatacttat
     2401 accggcggca ttgagcacgc caccatgcac ctgatataca cgcggttttt ccataaggca
     2461 tgtcgcgata tgggtatcgt tgagggtcct gaaccgatga gccaattgcg caaccagggt
     2521 atcgtgctgg gtgaagactc ggaaaagatg tcaaagagtc gtggaaatgt tgtcgctcca
     2581 gatgatctgg ttagccgcta cggagccgac acagtacgtg catatttgat gtttttcgct
     2641 cgctggaatt tgggcggtcc gtggaatagc agtggtattg agggcgcagc ccggtgggta
     2701 cgccgggtgt ggattatgct gaccgagccg gcgccggtgg caaatccctc ttcggaagtg
     2761 ttgcggtctc tacgcagaaa ggtacaccag actttacaga gcgtcacgct tgattttgaa
     2821 caattcgagt tcaacaccat aatctccagc ctgatggagt tgttaaatga gatgagcaaa
     2881 gcccgagagc aaggggctgg cggcacaccc gcgtgggatg aagcggttga tatctacttg
     2941 cggatgatgg ctcccatcac cccgcacatt gctgaagagc tgtgggctta tatggggaag
     3001 ccgtactcca tccatgcgca ggattggcca caagtcgatg aaagtgcagc aagcgaagaa
     3061 gagatcacgc tcgttgtaca ggtgaatggc aaagtgcgag accgtatcgt cgtaccggtt
     3121 aatattggtg aagatgaggc caaagctgcg gccctggc
//
LOCUS       DBNGSX010000201         1601 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_986020, whole genome shotgun sequence.
ACCESSION   DBNGSX010000201 DBNGSX010000000
VERSION     DBNGSX010000201.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_986020"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="AC16CB_06190"
     CDS             <1..726
                     /locus_tag="AC16CB_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3342091.1"
                     /translation="DGPDLASLLRITHQVEGLERIRFLTSHPNWMTDELLDTVAELPK
                     VMPHIEVPVQAGDDQVLENMKRGYTADDYRRLIQKIRERIPLVSIATDIIVGFPGESE
                     AQFQRTYDLLAELKMDVAHLARYSTRPGTVAERRMPDDVPDQEKWRRFRSLEELQENI
                     ATEINAGYLGEVVEVLFEEKVRGRWKGRTPTNKLVFVESKADLHGQVLPVEITWTGPW
                     SMQGRLKNQRGAEIPLDLVSISI"
     gene            1111..>1601
                     /locus_tag="AC16CB_06195"
     CDS             1111..>1601
                     /locus_tag="AC16CB_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR3342092.1"
                     /translation="MTLRTTLTTTIAILMLLVLSACSLPGSESEPTQVSVEAVYTAAA
                     QTLEARLTETAAAQPTEIPTDTPIPPTETPAETATPEFTPTASSTPEPTGPMITVRIG
                     SNCRKGPSTAYEPPITTLAEGQKAEIQGRNSNSSWWYVQIPGRPGQYCWIFGDNVSVQ
                     GEVT"
BASE COUNT          407 a          458 c          432 g          304 t
ORIGIN      
        1 gacgggccag acctggcctc cctactgcgc atcacccacc aagtggaagg tctggagcgc
       61 atccgcttct tgacatctca ccccaactgg atgactgacg agctgctgga cacggtagcg
      121 gagctgccaa aagtcatgcc gcatatcgag gtgcctgtgc aggcaggcga tgaccaggta
      181 ctcgaaaaca tgaaacgcgg ctacaccgcc gatgactacc gccggctgat ccaaaagatc
      241 cgggagcgca tcccgcttgt ctccatcgcc actgatatca tcgtcggctt ccccggcgaa
      301 agcgaagccc aattccagcg cacttatgac ttgctggcag aacttaagat ggatgtggct
      361 cacctagcgc gttactccac gcgcccgggg acagtcgccg agcggcgcat gcccgacgac
      421 gtgcccgacc aggagaaatg gcgacgattc cgcagcctgg aggagctaca ggagaacatc
      481 gccaccgaga tcaacgccgg ctatctgggc gaggtagttg aagtgttatt cgaagagaag
      541 gtgcgtggtc gctggaaagg gcgcacaccc accaacaaat tggtctttgt cgagagtaaa
      601 gcagacttgc acggtcaggt gcttccagta gaaatcacct ggactggccc atggtcaatg
      661 caaggccggc tgaaaaacca gcgtggggcg gagattccac tcgatctggt atcgatctcc
      721 atataaaaca ataaacttag gctctttcgg aagacataaa acaccctaaa tttaatatgt
      781 ttcgcagccg attttcccac cctttcgttc cccctcccac cgcctgcggc ttcgggaggg
      841 ggatgattaa cagaaaaacg ggttattggg cggcggaaac gccgcccaat aacccgtttt
      901 tccgatattt tccccgccct tcctgcgggg gagggcgggg ttaggggtgg gtgggggtag
      961 taataggtac gacccagtcc gaaagatcga atacaggagg cgtcgcgtac aattcttaat
     1021 cgaagtgcgg ttgcaaattt gctcatatca ttgtacactg agatttggaa cctgaaaggt
     1081 ggactcagcg tccatattca ggaaaggaga atgaccttgc gtaccacatt aacaactact
     1141 atagcaattc taatgcttct ggttttgagc gcctgcagct tgcctggcag cgaaagtgaa
     1201 cccacgcagg tatctgtgga agccgtctac acagccgctg cacagacctt ggaagccaga
     1261 ctcactgaga cggcggcagc ccagccgacc gaaatcccaa ccgacacacc cataccacca
     1321 acagagacac cagcggagac agcgactccc gagttcaccc caacggcttc ttctacaccg
     1381 gagccgaccg gccctatgat tacggtccga attggctcta actgccgcaa aggccccagc
     1441 accgcctatg aaccgccgat aaccacgctg gcagaagggc aaaaagccga gatacaaggt
     1501 cgaaactcga acagctcctg gtggtacgtg caaatccctg gcagacccgg tcaatactgc
     1561 tggatatttg gggataatgt cagcgtacag ggagaagtca c
//
LOCUS       DBNGSX010000202         1785 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1055389, whole genome shotgun sequence.
ACCESSION   DBNGSX010000202 DBNGSX010000000
VERSION     DBNGSX010000202.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1055389"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="AC16CB_06200"
     CDS             complement(<1..687)
                     /locus_tag="AC16CB_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HIR3342093.1"
                     /translation="MSLDFQQVRQQVKKLGEDAPSREDRLKALRQQARDLLQVNAGEL
                     ESLRQKVQAVVRNHDPSLRCAMPTFERLNASYPLPVIPSRATLLAADGSQIYFDRHSA
                     AEYFLINIGSIRMEYGNDQPPAISVLSDLHYGDDLHISGSYPSEEKISLLRDLRERQA
                     LADLAEQSNSPLIAMTDGSLELWGASPGSAAGQAGEEPDSLGAFLETLARQEAAGATV
                     AGYVDKPGEDY"
     gene            792..>1785
                     /locus_tag="AC16CB_06205"
     CDS             792..>1785
                     /locus_tag="AC16CB_06205"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3342094.1"
                     /translation="MAGFWCTIDAIFMASLTKLLASSSPSGRLRPVDIRRDLNPVADL
                     MENSFSETLDPDGRRYLQQMRSAANNPGFLRWAGLAAEGARVPFSGYVWEEDGQVVGN
                     LTLIPYYTLGLRYMLVANVAVHSNYRRKGIATSLTLKAIEHARGRGAQAIWLHVREEN
                     EGATRMYRSLGFVERARRTTWVTDEVQPPGSVGPVINQGVRITPGISVHPRRASDWRL
                     QHDWLEKLYPAQLSWHLSLKFNALRPDLWGLLIRVLNDIHVRQWSARQNDRLAGVLTW
                     QLMPAYADSLWLATAPEFEEDVAGALLQYARSHLPSRRPLSLDYPAERASQAIQA"
BASE COUNT          350 a          484 c          522 g          429 t
ORIGIN      
        1 atagtcctct cctggtttat ccacgtatcc tgccacggtt gcgccggctg cttcctggcg
       61 ggcaagagtt tcgaggaagg ctcccaggct gtctggctct tccccagcct ggcctgcagc
      121 tgacccaggg ctggcgcccc acagctccag gctgccatcc gtcatagcaa taagcgggct
      181 gttactttgc tcggctagat cagctagtgc ctggcgctcg cgcaggtcgc gcagcaagga
      241 gatcttttcc tcgcttgggt aagagccaga gatgtgcagg tcgtccccat agtgcaggtc
      301 gctcagcaca gagatggcag gtggctggtc attaccatat tccattcgaa tgctgccgat
      361 gttgatcagg aaatattccg ccgccgagtg gcggtcaaag taaatctgag agccatctgc
      421 tgccaatagg gtggcgcgcg acggtattac tggtagggga tagctggcat tcaggcgctc
      481 aaaggtgggc atggcacagc gcagagacgg atcgtgattg cgcacgactg cctgcacttt
      541 ctggcgcaag ctctctagtt cgcctgcatt gacctgaagc aagtctctgg cttgctggcg
      601 gagcgccttg aggcggtctt cccggctggg cgcatcctca cccaatttct tgacctgttg
      661 gcgcacctgc tgaaaatcta gcgacatggc gatcccttca tctcagaaca tttgtttgat
      721 caattgtacc agtttttgac taaatggctt taccatgatc atggtaaagt ccaaggagaa
      781 aattttgggc aatggctgga ttctggtgta caatcgatgc catatttatg gcctctctaa
      841 ccaagttgct cgcctcttcg tctccatctg ggagactgcg ccccgtagat attcgccggg
      901 acttgaatcc tgtcgctgac ctgatggaga actccttttc cgagacgctg gacccggatg
      961 ggcggcgtta cttgcagcaa atgcgttcag ctgccaacaa cccaggattc cttcgctggg
     1021 cggggttggc cgccgagggg gccagggtgc ctttctcggg atatgtgtgg gaagaagatg
     1081 gacaggtggt tggcaatctc acattgatcc cttattacac gcttggcctc aggtatatgt
     1141 tggttgccaa tgtcgccgtc cattctaatt atcgtcgcaa ggggatcgct accagcctga
     1201 ccttaaaagc gatcgagcac gcccgggggc gcggcgcaca ggctatctgg ttgcatgtgc
     1261 gtgaagagaa cgagggcgcg acccgcatgt atcgctcctt ggggtttgtc gagagggcac
     1321 gccgcacaac ctgggtaaca gatgaagtgc agccgcctgg ctcagtgggg cctgtgatta
     1381 accagggggt tcgcatcact ccgggtatca gtgtacatcc acgacgcgca agcgattggc
     1441 gccttcaaca cgattggctg gagaagttat atcctgctca gctgagctgg cacctgtcgc
     1501 tcaaattcaa cgccctgcgc cctgatttat gggggctatt gatccgcgtc ttgaatgaca
     1561 tccatgtgcg ccagtggtct gcgcgtcaaa atgatcggtt agcgggagtt ttgacttggc
     1621 agcttatgcc ggcgtatgca gatagccttt ggctggcgac agcgcctgaa ttcgaagaag
     1681 atgtggctgg tgcattgctt caatacgccc gctctcatct accttcgcgt cgaccgctgt
     1741 cgttagatta tccagccgag cgggctagcc aagcgatcca ggctg
//
LOCUS       DBNGSX010000203         6524 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_640070, whole genome shotgun sequence.
ACCESSION   DBNGSX010000203 DBNGSX010000000
VERSION     DBNGSX010000203.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6524)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6524)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6524
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_640070"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..696
                     /locus_tag="AC16CB_06210"
     CDS             232..696
                     /locus_tag="AC16CB_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012036844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HIR3342095.1"
                     /translation="MSHPCEITEQPAQPTLSIRTRAAVQNLPQVLGDGYGAIMQYLGE
                     LGISPTGHPFVIYYNMDMQDLDIELGFPVSQPLPGKGQIHAARMEGGKVAEYLYVGPY
                     ADCGPAYEELAQFVKDQGYETTGVAIEYYLNDPSQPPYEEPQTRIVLPLKSA"
     gene            925..1713
                     /locus_tag="AC16CB_06215"
     CDS             925..1713
                     /locus_tag="AC16CB_06215"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017601844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HIR3342096.1"
                     /translation="MSCKNYLVDMDGVLVRGTSTIPGAAQFIDRLVTCGCKYLILTNN
                     PLYTPGDLAHRLQVAGLDVPETHIFTSALATAQFLDRQKPKGKAFVIGESGLTSAIHS
                     VGYIITDLEPDYVILGETFAYNLELVTKAIRLISAGAKFIATNPDPSGPSESGLVPAC
                     GAMAALIEKASGVSPFFVGKPNPLMMRMALNYLDVHSENTVMVGDRMDTDIITGVMSG
                     METILVLSGVTKQGEIDRYPYQPTYIYDSVKDIEPCKPNKRVKE"
     gene            1717..1971
                     /locus_tag="AC16CB_06220"
     CDS             1717..1971
                     /locus_tag="AC16CB_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017291331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HIR3342097.1"
                     /translation="MSEEKVRTEEFEVSGDELVAKIKELVHEGNIRRISIRNEEGRTL
                     IDLPLTISVVGAILVPQLAALGAIAALVTHCTIVIEKVDE"
     gene            2442..5600
                     /locus_tag="AC16CB_06225"
     CDS             2442..5600
                     /locus_tag="AC16CB_06225"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3342098.1"
                     /translation="MQSNRLPNLQPKPAAAMVVGGGIGGMRAALDLAEAGLKVYLVEY
                     TPCLGGRVAQLGYMFPQHDCVLCRGTPDHGYGCTRPSLSPAYIQQNQHPNIEILTNTH
                     VVDVEGQAGDFTVSLRIEPTYVDAARCINCGYCAEACPVELPDSYQQGMVTRKAAYKV
                     AARAAPDAFVIDRGPYCDACGKCVEVCPSGAIDLEEQPRLISIAVGAIILALGFQIYD
                     PHLLGELGYGRFSDVVHAMEYERLASRSGPTEGTIVRPSNHEPPRSIAWLQCIGSRDT
                     DNSFCSSICCMYATKEAILAKQRLGGEVDCRIFIMDERAFNKEYSTYFSMARQKHGIQ
                     YTHCRVSAIQEDPLTHDLIVQYADPDGKSHQERFEMVVLATGLQPPESAHHFTQMLDL
                     ELNEHGFCHTDQFTPLQTTHSGVFVAGAFSSPKEISETILDASGAAAEVMRLLNERLN
                     TYTYTRKWPFITTEDLPPERDDSQEPLQVGVFTCSCGGTIGEAIDIPSLARQANGWPN
                     VTHVEAMDFACFPEGISQLQERIQEKGLNRVVVAACSNRTHDSLFQRAIRQVGLNPYY
                     LELVNLREQCSRVHVWQSEQANRKAQELVRIAVGRASKAKPVHKQLHQARPSALVIGG
                     GLAGMTAALAIGDSGYNVHLIERTEVLGGNLQNLYYVAEGYNPRRLGRDLINRVRAHS
                     RIAVLTRAELVRHEGHMGDFRSTLRVSRPDGSSEVITIEHGVTIVATGGHETRQHPWL
                     SLPGVITQRELEEMVIHRPKEIADLKDVVMIQCVQAEGAPQYCSRVCCTNTIKNAIRL
                     KLFNPNCRVSVLYKNIVTYGFREEFYNEARRLGVIFIRYTDDFPPELKITDKHIEVHA
                     RDLSLGRWLKLPADLITLSMPIIPSEGTRELAKILRVPLSTEGFFEEAQMKLRPMDFM
                     REGIFLAGMAHYPKFIEESITHALATAGRAVTLLSQSKALYLGGVVAEVDTSKCVGCL
                     TCTRTCPFQIPQVIEIEGRAGVGGLGGAAFIEPALCQGCGTCTGECPANAIQLVNYTD
                     EQVMLREVGGLGQWLPLIVTNN"
     gene            5670..6173
                     /locus_tag="AC16CB_06230"
     CDS             5670..6173
                     /locus_tag="AC16CB_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HIR3342099.1"
                     /translation="MSTEASTAFEPEITAFYCIYCGYMAADTAGALHIQYPANVKFVR
                     LPCTGKSDIRYFLEAFEQGADGVYVVACPLGNCHHVRGNERGHARLTRAKQILDSIGL
                     GGERLDMFFMSGSQGHSFAMAAQTMTERIRKMGPNPLKLQAEGKKIQAVTVEEEEENS
                     FRGRKKK"
     gene            6170..>6524
                     /locus_tag="AC16CB_06235"
     CDS             6170..>6524
                     /locus_tag="AC16CB_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3342100.1"
                     /translation="MTPVGAVMVVGGGIAGMQASLDLADQGFKVYLVETKSAIGGKMA
                     QLDKTFPTNDCAMCTISPKLVEVGRHPNIQVMTETEVLDIQGRAGDFTAYLEHHPRYV
                     DPNKCVSCGDCVKVCP"
BASE COUNT         1529 a         1798 c         1815 g         1382 t
ORIGIN      
        1 gatcattgtt tttgctgctg gcgcttttac cattttgccc ctggaagaat tggtcgtgct
       61 tttgggtggc aagccacctc aattttcatt aagtgggcac gaggctgacc catgatccca
      121 ctatctcaat ttgtctgcgg cagccgcacc aatcttgaca aaatagaacg catgtgctat
      181 tattcacaaa aggttttcgt ccaatctgta catctattgg gagggagtgc catgtctcac
      241 ccgtgcgaaa tcacggagca gcctgctcag cccacactga gcatccgcac cagggctgcg
      301 gtgcagaatc taccccaggt gctgggggat ggctatggag ccatcatgca atacctgggc
      361 gagctgggga taagcccaac aggacatcca ttcgtaatct attacaacat ggacatgcaa
      421 gatttagaca tcgagctggg cttcccagtg tcccaaccgc tgccgggcaa gggtcagatc
      481 cacgctgccc gcatggaggg gggtaaagtc gcggagtacc tgtatgtcgg tccctacgcg
      541 gattgcgggc ccgcttacga ggagttggcc caattcgtca aagatcaggg ttatgaaacc
      601 accggcgtgg ccatcgagta ttacctcaac gatcccagcc aaccacccta cgaagagcca
      661 caaacccgca tcgtcctccc gctgaagagc gcctgacaaa gagattttca ccaccaaggg
      721 cacaaaagta gcaaacatag aacaccccaa tttaataatt gttggatttt gtagttcgta
      781 tgcccacccc ctcgttcccc ctcccaccgc ctgcggcttc gggagggggg atgattgacg
      841 catatcacag tattccttat aatgagtttg ggcggtgaaa tcaccaacaa tttatctccg
      901 ctggatggca ctggagcatg gataatgtcc tgtaaaaatt acctggtcga tatggatggg
      961 gtgctggtac gcggcaccag cactatccct ggcgcagccc agtttatcga tcgcctggtg
     1021 acctgcggct gcaaatacct gatcctgacc aataacccgc tttacacgcc gggcgatctg
     1081 gctcaccgcc tccaggtagc cgggttggat gtcccagaga cgcatatctt cacctctgcg
     1141 ctggcaactg cccaattcct ggatcgccag aaacctaagg gcaaagcctt tgtgatcgga
     1201 gagagcggtc tgacttccgc catccacagt gtgggctaca tcattacgga cctcgagccg
     1261 gattacgtca tcctgggcga gacctttgct tacaacctgg agcttgtcac caaagcgatc
     1321 cgattaatct cggcaggggc gaagttcatc gccaccaatc cagacccatc cgggccttcc
     1381 gagagcgggc tggtccccgc ttgtggggct atggcagccc tgatcgagaa agccagcggg
     1441 gtctcaccgt tctttgttgg caagccgaac ccgttgatga tgcgcatggc gctcaattat
     1501 ctggatgtcc attccgagaa caccgtgatg gtcggcgacc gcatggacac ggatatcatt
     1561 acaggtgtca tgtctggaat ggaaaccatt ttggtgctca gcggggtaac aaaacaaggc
     1621 gagatagacc gttatccata ccagccgacc tatatctatg attcagtgaa ggatatcgaa
     1681 ccgtgcaaac cgaataaacg cgtaaaggag taaagcatgt ctgaagagaa agtccgaacc
     1741 gaagagtttg aggtcagtgg cgacgaactg gtagccaaga tcaaggagct ggtgcatgag
     1801 ggcaatatcc ggcgcatcag catccgcaac gaggagggcc gcaccctgat cgacctgccg
     1861 ctcaccatca gcgtggtggg ggcgatcctg gtgccgcaac tggcggcgct cggcgcgatt
     1921 gccgcactgg taacgcattg cacgatcgtt atcgagaagg tggacgagta gagccggaat
     1981 catcctcccg caggttttaa acctgcggga ggcaggggga ataacaagat tttgttggtc
     2041 taattaggac aactatgaca aatatcacta atcttgtagg attctcgacc cgttgacaag
     2101 ccaaggttat ttgctacaat aaaatacgag aaactgtgca agatttcaca gcaaatttct
     2161 aagcgctagc taccctctac accaagggct gacaacaaac cttgttgccg aatccgccct
     2221 ttctgcactg gcagggaggg cggtttgcgc ttatagcaga ccttaataac tttgaacaaa
     2281 gaccctaaag gtgacgagcg cctttagggt ctggctcaga tcaaactaac ctcggcaggg
     2341 ggacgttcat ccgttcgatg gtattgcttt agtccctatg ccgtatggtt tatttggcag
     2401 atcgctattc gctacgcacc gcatagcttg gaacatacct tatgcagtcg aaccgtttgc
     2461 ccaacttaca acctaagcct gccgccgcca tggtggttgg cggcggaatc ggcgggatgc
     2521 gcgcggcctt agatctggca gaagctggat tgaaagtcta cctggtggaa tacacgccat
     2581 gcctgggtgg gagagtggca caacttggat acatgttccc acaacatgac tgcgtgctct
     2641 gccggggcac acctgaccat ggttacggct gcacccgtcc gtcgctttcc cccgcgtata
     2701 tccagcaaaa ccaacatccg aatatcgaaa ttctcaccaa tacccacgta gtggatgtgg
     2761 aaggacaggc tggtgatttc actgtctcgc tgcgcatcga gccaacctac gtcgatgcgg
     2821 cgcgctgtat caactgcggc tactgcgccg aagcctgccc ggtggaatta ccagacagct
     2881 accagcaagg catggtgacg cgcaaggcag cctacaaagt cgcggcacgc gccgcaccgg
     2941 atgccttcgt catcgatcga ggaccgtatt gcgatgcttg cggcaaatgt gtcgaagttt
     3001 gccccagcgg ggcgatcgat ctggaggaac aaccgcgcct gatctccatc gccgtgggag
     3061 ctatcatcct ggcgcttgga ttccagatct atgacccgca cttgctggga gaactggggt
     3121 acgggcgatt ttcggatgtg gtgcacgcca tggagtacga acgtctggcg agccgctccg
     3181 ggccaacaga aggtaccatt gtgcgcccct ccaaccacga gccgccgcgc tcgattgcct
     3241 ggttgcagtg tattggctcg cgcgacacgg acaattcctt ctgctcatcc atctgttgca
     3301 tgtacgccac caaggaagcc atcttggcca aacaacgcct ggggggtgag gtggactgcc
     3361 gcatctttat catggatgag cgagccttca acaaagaata cagcacttac ttctctatgg
     3421 cgcgccagaa gcacggtatt cagtatacgc attgccgtgt gtcagctatt caagaggatc
     3481 cactcactca cgacctgatc gtgcaatacg ccgacccgga tggcaagtcc caccaggaac
     3541 gctttgagat ggtggttctc gccaccggtt tgcagccccc cgaatcggcg caccatttca
     3601 cccagatgct cgacctggag ctgaatgagc atggcttttg ccacacagac caattcaccc
     3661 ccttgcagac gacccactct ggggtcttcg tagccggggc attctcgtcg cccaaggaga
     3721 tttccgagac catcttggat gccagcggag cagcggcaga ggtgatgcgc ctactcaatg
     3781 agcgcctgaa cacctacacc tatacccgca agtggccgtt tatcacaacc gaagacctgc
     3841 caccggaacg cgatgatagc caggaaccgc tgcaagtagg agtatttacc tgctcctgcg
     3901 gcggcacaat tggggaggcg atcgacatac cttccctggc acgccaggcg aatggctggc
     3961 cgaatgtgac ccacgtcgag gcgatggact tcgcctgttt cccagagggg atcagccagc
     4021 tgcaagagcg aattcaagag aaaggtttga accgtgtcgt ggtagccgca tgtagcaacc
     4081 gcacacacga ctcgctcttc cagcgcgcca ttcgccaggt tggtctgaat ccctactatt
     4141 tggaattggt gaacttgcgt gaacaatgca gccgggtgca cgtctggcag tccgagcagg
     4201 ccaaccgtaa ggcacaggag ctggtacgca ttgccgttgg gcgcgcttca aaagcgaagc
     4261 cagtgcacaa gcaactgcac caagcccggc cctccgccct ggtaatcggg ggaggcctgg
     4321 caggcatgac cgcagccctc gctatcggcg acagcggtta taacgtgcac ctgattgaac
     4381 gcaccgaggt cttgggcggc aacctacaga acttgtatta cgtcgccgaa ggctataatc
     4441 cacgtcgctt ggggcgcgat ctgatcaacc gcgtgcgcgc ccactcgcgc attgctgtct
     4501 taacccgggc agagctggtg cgtcacgagg ggcatatggg tgacttccgc tcaacgctgc
     4561 gtgtctcgcg tccagatggc tcttctgagg tgattaccat cgagcatggc gtgaccattg
     4621 tcgccaccgg cggtcatgag acacgccagc atccctggct aagcctgcca ggggtgatca
     4681 cccagcgcga actcgaagag atggtcatcc atagacccaa ggagatcgcc gatctaaaag
     4741 atgtggtgat gatccagtgc gtccaggcag aaggagcccc ccagtactgc tcgagagtgt
     4801 gctgcaccaa caccatcaag aacgccatcc ggctgaagtt atttaatcca aactgccggg
     4861 tgagcgtgtt gtataagaac atcgtcactt atggcttccg cgaagagttc tacaatgaag
     4921 cgcgccgttt aggtgtgatt tttatccgct acacagacga cttcccaccc gaacttaaaa
     4981 tcacggacaa gcatattgag gtgcacgccc gtgacctctc cttgggtcgt tggttgaagc
     5041 tcccagccga tctgattacg ctgagcatgc cgatcatccc atcggaaggg acaagggagc
     5101 tggcaaaaat cctgcgcgta ccattgtcca ccgagggctt cttcgaagag gcacagatga
     5161 aattgcgccc gatggacttc atgcgtgagg ggattttcct ggctggcatg gcacactacc
     5221 ccaagttcat tgaagaaagc atcacccacg ccctggcaac ggctggtcgc gccgtgacct
     5281 tgctatccca gagcaaggcg ctttacctgg gaggcgtggt ggcggaagtg gatacatcca
     5341 aatgcgtggg ctgcttgacc tgcacgcgca cctgcccctt ccaaatcccc caggtgatcg
     5401 aaatagaagg acgagcgggc gtgggcggcc tgggaggcgc agccttcatc gaaccggcac
     5461 tgtgccaggg ctgcggtaca tgcactggag agtgcccagc caacgcaatt caactggtca
     5521 actacactga tgaacaggtc atgctgagag aggtcggcgg attggggcag tggttgccac
     5581 tgatagtgac aaataactaa aacatatctc aaaacaaggt gcgtgagtgt ttatgagatg
     5641 agctctaagg atagttgagg tttgatttga tgagcactga agccagtacg gcgtttgaac
     5701 ccgagatcac tgccttctac tgcatttact gcggttatat ggcagcagat acagcgggtg
     5761 cgctgcacat ccaatatccg gctaacgtca agtttgtgcg cctgccctgt acaggcaagt
     5821 cggatattcg ctatttcctg gaggcttttg agcagggcgc cgatggggta tatgtggtgg
     5881 cctgcccgct aggtaactgc caccacgtgc gcggcaatga gcgagggcat gcgcgcttga
     5941 cgcgagccaa acagatactg gattcgattg gcctgggtgg tgaacgcctg gatatgtttt
     6001 tcatgtcggg cagccagggg cattccttcg ctatggcagc ccagaccatg accgagcgca
     6061 tccgaaagat gggtccaaat cccttgaaat tgcaggctga aggtaaaaag atacaagctg
     6121 taacggtaga agaggaagaa gagaacagct tccgtgggag gaagaaaaaa tgacacctgt
     6181 tggtgcggta atggtagtgg gcggcggaat tgccggtatg caagcgtctt tggatttggc
     6241 cgatcagggc ttcaaagttt atctggtaga gactaaatca gctattggtg ggaagatggc
     6301 ccagcttgac aagaccttcc ccaccaatga ctgtgccatg tgtaccatct cacccaagct
     6361 ggtggaggtg ggccgccatc ctaacatcca ggtgatgact gaaaccgaag tgctggacat
     6421 tcagggacgg gcaggtgact tcaccgcgta cctggaacac cacccgcgct atgtagaccc
     6481 caacaaatgt gtctcctgtg gcgactgcgt caaggtatgc ccgg
//
LOCUS       DBNGSX010000204         9751 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1747334, whole genome shotgun sequence.
ACCESSION   DBNGSX010000204 DBNGSX010000000
VERSION     DBNGSX010000204.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9751)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9751)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9751
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1747334"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="AC16CB_06240"
     CDS             complement(<1..1000)
                     /locus_tag="AC16CB_06240"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HIR3342101.1"
                     /translation="MPPVVYLIDGHALAYRTYFALTRGSSQGFTTSKGEPTAGVFGFT
                     SVLLRILEQERPDYLAVAFDTGKTFRDELFPEYKGTRAKMPDDLRPQIERIRQLVDAF
                     NIPRLEVENYEADDVLGSVAHWAVENGLGVKIITGDRDLLQLVGERVIVNLPGKSLSE
                     ARDYLPKDVAEVMGVQPEQVVDFKALVGDKSDNIPGVMGIGEKTAASLLQTYHDLDNL
                     YAHLDEIRGSVREKLEKGREDAYLSRTLAKIVTDLNVSLDLEQARPQHFEPAVIEAIF
                     RELEFRTLMARLTALMQSYGMNAVQKGQQLSLFAEAAPAPQDKAILGEQAGLQTRIV"
     gene            1213..2829
                     /locus_tag="AC16CB_06245"
     CDS             1213..2829
                     /locus_tag="AC16CB_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342102.1"
                     /translation="MPSVSRAIKIFVIFLVVIGAAIWITYRVAADEPGRQMTIVIPYI
                     EYEYWLLSWQENEVECRVVIDHEGLPTSQDVLKSCGEGVQTEWLNTPSCNKIIKGKGK
                     ISSCPGLYLIQISSQPKEKEITIDLPQASVGVNLEGCSPVPPENLCEKLPALLLTGEE
                     PLPNEQITSIEGTINGVPFNCKGATCPVKLTITSDIGVTVEFWANSTFGDSSPRYTAQ
                     VRVIDAGVSLVPGGGGFHIDVLSSQWKGGPLASCSRTWETFPPVGGTVDWLRSPNQPA
                     LLVSDEAYYYLAGRLIAQGVVDASKCPSLGLLPNGYANACGLEVAAPIVMAWQNQFDA
                     RIIQVSKETGVPAQLLKNLFAQESQFWPGMFKVPFEFGLGQITDKGADTILLWNTDFY
                     ETFCPLVLSKKACDNGYLKLQVDDQAMLRGALAQQAKSDCPLCPAGVDLTSANSSISL
                     FANTLLSSCDQVAQIVHNATGEKPGAVSSYEDLWRFTIANYHAGPGCLSYAIYATWAA
                     NGQELLWDEVTTQFTEACKGVIPYVDKIAR"
     gene            complement(2833..3021)
                     /locus_tag="AC16CB_06250"
     CDS             complement(2833..3021)
                     /locus_tag="AC16CB_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342103.1"
                     /translation="MAKEKDLPEPSTDVIAETDNYVAWRAKEPDGETTYHLELASVTV
                     HFFQEEWDEFLELVEALV"
     gene            complement(3173..4243)
                     /locus_tag="AC16CB_06255"
     CDS             complement(3173..4243)
                     /locus_tag="AC16CB_06255"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR3342104.1"
                     /translation="MSPKSGHIGSIVAWIVALSLVLFAAYLGWQMVPSKATEPVQNAS
                     AETIAFLEEPLDPMVSLPIYAHAKYSHALVRKVVPRTVIPSRPSLEVKSYTVELGDSV
                     FEIARNFKIKPETLLWANYDQLNDNPDMVSIGMNLSIPPVDGVYYQWQDGDTVQSIAT
                     RFEAKIDDILNWSGNNLDLTEPTKSPGEWVMVPGGHREFRQWIIPVIPRGAAGVSKSV
                     YGAGACEGGYEGAFGSGGFIWPAGNHSLSGNDYWSGHLGIDIAAGEGAPIYAADSGVV
                     VFSGWATGGYGNMVMVDHGNGYQSVYAHLSNASASCGRSVGQGQIIGYSGSTGNSTGA
                     HLHFEVRYLGGFVSPWYVLPAP"
     gene            complement(4282..5562)
                     /gene="rho"
                     /locus_tag="AC16CB_06260"
     CDS             complement(4282..5562)
                     /gene="rho"
                     /locus_tag="AC16CB_06260"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR3342105.1"
                     /translation="MKVIELENIPLNALRQKAKELDVPNALRLKKEDLIMRIQQTEAE
                     REGLEVRGGILEIMSEGIGFLRSAHYQPGPYDVYVSQSQIRRYNLRNGDLIIGNVRPP
                     RESEKHYGLLKVESVNGLDPEIARHRPAFESLTPIFPDTRFDLELDRHTLATRLINLV
                     TPIGRGQRGLIVSPPKAGKTTILKQCANAISNKYPEVHLMVALIGERPEEVTDMDRSV
                     DAEVISSTFDEPVTSHVRVAEMALERAKRLVEVNRDVVILMDSITRLARAYNLVVTPS
                     GRTLSGGIDPSALYPPKRFFGAARNIEEGGSLTIIATCLVDTGSRMDDVVYEEFKGTG
                     NMELHLSRRLQERRVFPAIDIERSSTRREELLLGPDITPRVWLMRRMYDQMVTAPPHG
                     AGLDPSTATEAILQRLARTSNNLEFLETLTEDNR"
     gene            complement(6075..7682)
                     /locus_tag="AC16CB_06265"
     CDS             complement(6075..7682)
                     /locus_tag="AC16CB_06265"
                     /inference="COORDINATES: protein motif:HMM:NF025141.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3342106.1"
                     /translation="MTYRDRDDPVDPDGFFLIMLAPRPEAAIETLSKDVILVLDRSGS
                     MDGEKFRQAQEALRYILRHLNPDDRFNIIVFSTGLETYGRNLRPAEEANEAIAWVDRQ
                     SAQGSTDINRALLEAAAMSDRERPTYLIFLTDGLPTEGIVESQDILDNLDSTAPDNLR
                     LFAFGVGYDVDTFLLDSLAQAHHGSSSYVLPGERLDEVLSAFYAKISSPVLTDLSLDF
                     EEIRTYDIYPDPLPDLFAGSQIIVVGRYREGGIADIVLRGKVEGRRQTFRFDEQRFAD
                     DDDEIRSSVLDAIPRLWATRKIGYLLNQIRLNGPDKETIDQIVRLSIRYGIVTPYTSY
                     LVTEEMPLGEAEQERIAREQYNQLSQMAPVPSSGQGAVERAAEQGGMAAADSAAPPSE
                     EAAQTVRIVGSKTFVVTNGVWVDTGYDPMAMQTVEVAFLSDDYFALVASRPKLAAAFA
                     LGRRVIALEEGIAYEVVEGTTEVKPIKIPPTHTPVPQPTGTPFPTLEPVIEITAEVTP
                     TAPGTMPCASGLLPLGLLVIWLRRKRE"
     gene            complement(7713..8474)
                     /locus_tag="AC16CB_06270"
     CDS             complement(7713..8474)
                     /locus_tag="AC16CB_06270"
                     /inference="COORDINATES: protein motif:HMM:NF020076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HIR3342107.1"
                     /translation="MKFKLALMLLFVFTLAALPTSARADGIIIPDPPICLDGPCPPPP
                     CPRPFPCPPRPSFVQLAIRYHRVSVTIQDQLVVTHVDQVFHNPNDWQVEGTYIFPIPV
                     DAAVSSFSLWIDGKPVEGKVLDAEEARKTYEEIVRSMSDPALLEYVDRGAVQARIFPI
                     PPGGERRIELEYVQALTAENGLVRYTYPLSTEKFSLWPLENVSISVEVRSSQAPIRAV
                     YSPSHEIAINRLDERHVMAGYEDVDVLPDTDFTLY"
     gene            8685..>9751
                     /gene="prfA"
                     /locus_tag="AC16CB_06275"
     CDS             8685..>9751
                     /gene="prfA"
                     /locus_tag="AC16CB_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR3342108.1"
                     /translation="MLEKLAGIESRYQELNQLLNEVGDDYQRAAELNKERIDLEPLYS
                     KAQEYRKSLDQLEQARHLLDTDDEELRSLAETEFTELEELIFQLEDEIKAMLLPKDRR
                     DQRNVIVEIRAGTGGEEAALFASDLFRMYSRYAERRRWDVDILSANETGIGGFKEIIF
                     MVKGRSAYSRLKYESGVHRVQRIPVTEASGRIHTSTATVAVLAEVDEVEIDIPDSDIR
                     MDVFKSAGAGGQNVQKNATAVRITHIPTGIVVACQDERSQLQNRMRAMSILRARLYEI
                     EEEKRRLEVDETRRSQVGTGERAEKIRTYNYPQSRVTDHRINISNFNLPMFIDGDIDE
                     FIDELATRDEAARLAAVGLEI"
BASE COUNT         2452 a         2574 c         2480 g         2245 t
ORIGIN      
        1 ccacaatcct ggtttgcagg cctgcttgct ctcccagaat agctttgtcc tgtggagctg
       61 gtgctgcctc agcgaacagg gatagctgct gacctttttg cactgcgttc atgccgtagg
      121 attgcatgag tgcagtaagg cgcgccatca atgtgcgaaa ctccagctca cggaaaatgg
      181 cttcaatgac agcaggctca aaatgctgcg ggcgagcctg ctccaggtca agggaaacgt
      241 tcaggtcggt aacaatcttc gccaaagtgc gacttagata cgcgtcttcc cggcctttct
      301 ccaacttttc ccgcacgctg cctctgattt catccaagtg agcatacagg ttatccaggt
      361 catgataggt ctggagcagg gaggcagctg tcttttcacc gatgcccatc acgccaggga
      421 tattatctga cttatcccca accagcgctt tgaaatcgac aacctgctct ggctgaacac
      481 ccatcacctc agccacatct ttcggcagat agtcgcgcgc ctccgacagc gatttacctg
      541 gtaaattgac gatgacccgc tcgcccacca gctgtaatag gtcacgatca ccagtgatga
      601 tcttgactcc caatccattt tctaccgccc aatgtgccac gctgcctaga acatcatcgg
      661 cttcgtaatt ctccacctcc aggcgaggga tattgaaggc atctaccagc tggcggatac
      721 gctcgatttg cggtctcaga tcatcgggca ttttggcgcg cgtgccctta tattccggaa
      781 acagctcgtc tcggaaggtt ttgccggtat caaaggcgac tgccaggtaa tcggggcgct
      841 cttgttccag gatgcgcagc agcacgctgg taaagccaaa aacgccagca gtcggctcac
      901 ccttgctggt ggtaaaccct tgcgaggagc cgcgggtaag tgcaaaataa gttcggtatg
      961 ccagtgcgtg accgtcgatt aaatagacaa cagggggcat aagctaccta cggtataaga
     1021 gtgtatcacg ggctttagac agccgcaagg acgttctaac caatatgtaa tgcccgtatt
     1081 tgtcaacgcg aataaattat tacctcaatg atagaggaag tgtaacgaac agcctgcacg
     1141 ttcgttctaa taataaaggc ttaaaattcc ctgattgaca atagtgcctg atcatgatat
     1201 ttttgttgta agatgcccag tgtctcgcgc gccatcaaga tctttgtgat cttccttgtc
     1261 gttatcggag cagcgatctg gatcacttat cgcgttgctg cggatgagcc gggacgccaa
     1321 atgacgattg tcattcccta tatcgagtat gagtactggc tcttatcctg gcaagagaat
     1381 gaagtagaat gccgggtggt gatagatcac gaggggttac caaccagtca agatgtgctc
     1441 aagtcttgcg gggagggtgt acaaacagaa tggctgaaca cgccgtcatg caacaagata
     1501 attaaaggga aaggcaagat ttcctcttgt ccaggcctat atctaatcca gatctcctct
     1561 cagcccaaag aaaaagaaat cacgatcgac cttccccagg cttcagtcgg agttaacctg
     1621 gaagggtgct ctcctgtccc accagagaac ctgtgcgaga aattgcctgc attgctgtta
     1681 actggggaag agccccttcc aaacgaacag attacatcca ttgagggtac catcaacggc
     1741 gtacccttca attgtaaggg ggcaacctgt ccggtgaagt tgacgatcac atccgatatt
     1801 ggggtgacgg tcgaattttg ggctaattct acgttcggcg actccagtcc acgctacacc
     1861 gcccaggtga gggttatcga cgccggagtt tccctggtcc ctggtggcgg gggattccac
     1921 attgacgtac tcagcagtca atggaaaggc ggtccgctcg ctagttgctc gcgtacctgg
     1981 gaaacttttc ctccggtggg cggcaccgta gactggttga ggagccccaa tcaacccgct
     2041 ttactggtat ccgatgaggc ttattactac ctagccggga gattgatcgc ccaaggggtt
     2101 gttgatgcat ctaaatgccc atcattggga ctgctaccca atggatatgc caacgcttgt
     2161 ggattggaag tagctgcccc aattgttatg gcctggcaaa accagttcga cgcacgcatc
     2221 atccaggtat ccaaagaaac cggcgtaccc gctcaattac tcaaaaacct gtttgcgcag
     2281 gagagccaat tttggcctgg gatgttcaaa gttccgtttg agtttggctt ggggcaaatt
     2341 acagataaag gagcagacac catactgctt tggaacacag atttctacga gacattttgt
     2401 ccgttggtcc taagcaagaa agcctgtgac aatggttacc taaagctgca agttgatgat
     2461 caggctatgt tacgtggagc actggcgcag caggcaaagt ccgactgccc gctttgtcct
     2521 gcgggtgtgg atttgaccag cgcgaactcc agcatctccc tgttcgccaa cacgctgcta
     2581 tcgagctgcg atcaggtagc tcaaattgtc cataatgcga cgggcgaaaa gccaggagcg
     2641 gtgagcagct atgaagattt gtggcgtttc accatcgcca attaccacgc cggcccaggt
     2701 tgcctgtcgt atgccattta tgctacctgg gctgcaaacg ggcaggaact actctgggat
     2761 gaggtaacca cccagttcac cgaggcctgc aaaggcgtga taccatacgt ggataaaatt
     2821 gcccgctgac ggttagacca gagcttccac cagctctaaa aactcatccc actcctcctg
     2881 aaagaagtgc acagtaacac tggcgagttc caagtggtaa gttgtttcgc catctggctc
     2941 ttttgcgcgc caggcgacat agttgtcagt ctctgcgatg acatcagtgg atggttcggg
     3001 aagatctttt tcttttgcca catcaccctc caattctttc ttccataatc attaggcgct
     3061 cgttgttcat ggtggagcgc ttttctttgg ggattacctc actccaatgc actcgcccag
     3121 cactgtaata ggttcgataa caccagattt gggcgtgtta cctattttat ccttacggag
     3181 ctggcaaaac ataccacgga ctgacaaagc ctcccaggta acgcacttca aaatgtagat
     3241 gagctccggt tgaattacca gtgcttcctg agtagccgat gatttgacct tgacccacgc
     3301 tacgcccgca ggaagcgctg gcgttgctca gatgggcata tacactctgg taaccgttgc
     3361 catggtcaac catgaccatg ttgccgtatc caccggttgc ccagccagag aatactacca
     3421 caccgctatc agccgcatag atcggggcac cctctccggc tgcaatatca atccctaggt
     3481 gaccagacca gtagtcattg cccgagagag aatgattgcc agcaggccat ataaaccctc
     3541 cactgccgaa tgctccctcg tagccacctt cgcaagctcc agcgccatac acggatttgg
     3601 atacccctgc cgcgccgcgc gggattacag ggataatcca ctggcggaac tcgcgatggc
     3661 ctcctggcac cattacccat tcaccaggcg atttggtcgg ttcagtcagg tccaaattat
     3721 tgcctgacca atttaatata tcatcaattt ttgcttcaaa acgagttgca atcgattgga
     3781 ctgtatcccc gtcctgccac tggtaataca cgccatcaac tggtgggata ctcaagttca
     3841 tcccaatcga aaccatgtcc ggattatcgt ttagttggtc gtaatttgcc cagagtaacg
     3901 tttcgggctt aatcttgaaa ttgcgagcaa tttcaaacac cgaatccccc aactcaacgg
     3961 tgtacgattt gacctctaag cttggacggc ttgggataac ggtgcgggga accactttcc
     4021 gcacaagtgc atgggagtac ttagcatgag cgtatattgg aagtgaaacc atcggatcaa
     4081 gcggttcttc caggaatgca atggtttcag ctgaagcatt ttgcaccggt tctgttgcct
     4141 tactgggaac catttgccaa cctaaataag cggcaaacag caccaggctc aatgcaacga
     4201 tccaggcaac aatggaacct atatgaccag atttggggga catgaaaggg ctaccgcggt
     4261 aagaccgcag atcttagcag attatcgatt gtcttcagtc agtgtttcca agaactccag
     4321 gttgttggaa gttcgtgcca gacgctgcaa gatagcttcg gtagctgtcg agggatctaa
     4381 tcctgcgccg tgcgggggtg cggtgaccat ctggtcgtac atccgccgca ttaaccaaac
     4441 gcgcggagtg atatcggggc ctaagagtaa ttcctcgcgc cgtgtagaag agcgctcgat
     4501 gtcgatagcc ggaaaaactc gccgctcctg aaggcgccgg gacaagtgca actccatatt
     4561 acccgtgcct ttgaactcct cgtacaccac atcatccatg cgcgagccgg tatcgaccag
     4621 acatgtagcg ataatcgtca aagatccacc ctcttcaata ttgcgggcag cgccaaaaaa
     4681 gcgcttaggc gggtaaagcg ccgaaggatc gataccgcca gacaatgtac ggcctgaagg
     4741 ggtaacgact aggttataag cccgcgccag ccgggtaatt gagtccatca aaatgaccac
     4801 atcccgattt acttccacga gccgcttggc gcgttccaag gccatttccg ccacacgcac
     4861 atgcgaggta acaggctcat cgaatgtaga cgagatcacc tcagcatcca ccgaacggtc
     4921 catgtcagtg acctcttcag ggcgctcacc gattaacgcc accatcaaat gcacctcagg
     4981 atacttattt gagatagcat tagcacattg ctttagaatg gtggttttgc ctgctttagg
     5041 cggcgaaaca atgaggccac gctgcccacg cccgatggga gtcaccagat tgatcagacg
     5101 ggtggccagg gtatggcgat ctaactccag atcaaagcga gtgtccggga aaatcggggt
     5161 gagcgattcg aaagccggtc tatggcgagc gatctcaggg tccagtccgt tgacactctc
     5221 gactttcagc aagccgtaat gtttttcgga ttcgcgcggg ggtctgacat taccgatgat
     5281 taaatcacca tttcgcagat tatagcggcg gatctgggat tgtgagacat acacgtcgta
     5341 tggacctggt tgataatgag cagagcgcag aaaaccgatg ccctcactca tgatctccag
     5401 tatgccacca cggacttcca gaccttcacg ttccgcttca gtctgctgga tccgcatgat
     5461 gagatcttct tttttcaaac gcaacgcatt tgggacgtcc aactctttcg ccttctggcg
     5521 aagagcattc agggggatat tttctaattc tatgaccttc atttttagtg actcgcttta
     5581 atatctaata gcatggatta ggtaccaccg ccaggactag ctcacttgcc ggcatactag
     5641 acggggggcc aataagcaca atggcaaaat caaaggggaa gcattaagat aaaaggagaa
     5701 attgcgtagg aattatagca tgcatggtaa ttacccgcaa gtctctcaga taaccaaatt
     5761 tgacaaaaaa aggaatctcc agtcaaacgg caagtgccac taacgcaatt tgctttcgct
     5821 tatatatgca agccccgatt taatatacta gtacggaatc acgccccaga ggtatattca
     5881 ttatacacta attctctttg cgcgcaagtc gcagggggca aaaatttcaa gattgttaga
     5941 cggcttaatc actcttgttt ctaacctgct caattcatta gataggcaaa ttcgaattga
     6001 ataggaagcg aaacacgaac tagggtgtct tgataaaaca taacacccta gttaatgtcc
     6061 ttcagcgaga aaaactactc cctttttcgc cttagccaga tgacaagcaa tcctagaggc
     6121 aataatccac tggcgcaggg catggtgcca ggagcagtgg gtgtgacctc cgccgtaatt
     6181 tcaatcacag gctctaacgt tgggaagggc gtacccgttg gttgcgggac aggcgtatgg
     6241 gttggcggga ttttgatggg cttcacctct gtagtgcctt ccacaacttc gtaagcaatt
     6301 ccttcttcta atgcgatcac tctccgcccc agagcaaatg ccgccgccag ttttggacga
     6361 gaggccacca gggcaaaata atcgtcagat aggaatgcca cttcaactgt ttgcattgcc
     6421 attggatcat aaccggtatc cacccagacg ccgttggtaa ccacaaacgt cttcgagcct
     6481 actatgcgca cagtctgtgc tgcttcttcc gacggtggtg cagccgagtc ggctgcggcc
     6541 ataccgccct gttcggcagc tctctcgacc gccccctggc cactggacgg cacaggcgcc
     6601 atctgagata gctggttgta ctgctcgcga gcaatgcgct cttgttcggc ttctcccaag
     6661 ggcatttcct cggtcaccag gtaggatgtg tagggagtca caatgccata gcggatgctc
     6721 aggcgcacaa tttgatcgat agtttcctta tccgggccat tcagacggat ttggttcaac
     6781 agataaccga ttttgcgtgt ggcccacagg cgtggaatgg catcgagtac agaacttcga
     6841 atttcgtcat cgtcatcggc aaagcgctgc tcatcgaagc gaaaagtttg cctgcgaccc
     6901 tctaccttgc cccgcaatac gatatcggcg atgccaccct cacggtaacg ccccaccaca
     6961 atgatctggg acccagcaaa caggtcggga agcggatcag gatatatatc ataagtacgg
     7021 atctcctcaa agtccagaga taggtcggta agcaccggag aactgatctt tgcgtaaaag
     7081 gcagacaaga cctcatccag acgctcgcca ggcagcacgt atgaactgga gccatgatgg
     7141 gcttgcgcga gggaatccag caaaaaagtg tccacatcgt accccacacc gaaagcaaaa
     7201 aggcgcaggt tatcaggtgc agtagagtcc aggttgtcca ggatatcctg gctctcaacg
     7261 atcccttctg ttggcagtcc gtcggtgagg aagatgaggt atgtgggtcg ctcgcggtca
     7321 gacatagcag ctgcctccag caaggcacgg ttgatgtctg tgctgccttg agcagattgc
     7381 cggtccaccc aagcgatggc ttcattggcc tcctccgcag gtcgcagatt ccgtccatag
     7441 gtctccagtc cggtgctgaa gacgataata ttaaatcggt cgtctggatt gagatggcgg
     7501 aggatataac gcaatgcttc ctgcgcctgc cggaactttt caccatccat gctgccggaa
     7561 cggtccaaca ccagaatgac gtctttggag agtgtctcaa ttgcggcttc aggacgtggg
     7621 gcaagcatga tcaggaagaa accatccggg tccactgggt catctcgatc gcggtaggtc
     7681 aacaggtgga aagcctgctc gtcgcccagg gactaataca aagtgaagtc agtatctgga
     7741 agtacgtcca catcttcata gccagccatc acgtggcgct cgtccaagcg attgattgcg
     7801 atttcatggc tgggcgagta cactgcgcgt atgggtgcct gcgatgagcg cacttccaca
     7861 ctgatgctta cattttctaa gggccagagc gagaatttct ccgtactcaa gggatatgtg
     7921 tagcgcacca gaccattctc agcggtaagc gcctggacgt actccagttc aatccgccgc
     7981 tcaccgcctg gcggtatagg aaagatgcgc gcttgcaccg caccacgatc gacatattcc
     8041 aaaagggctg ggtcgctcat gctgcgcacg atctcctcgt acgtcttgcg cgcctcttcg
     8101 gcatccagta cctttccctc aacaggcttg ccatcaatcc aaagggaaaa ggacgaaacg
     8161 gctgcatcta cagggattgg gaagatatag gtgccttcga cctgccaatc attggggtta
     8221 tggaaaacct ggtctacatg ggttaccacc aattgatcct gtattgtcac cgagacacga
     8281 tgataacgga tcgccagctg cacaaaggat ggtctggggg ggcacgggaa tggtcttggg
     8341 cagggtggcg gtgggcaagg tccgtccaaa catatcggcg ggtcggggat gataatgcca
     8401 tccgccctgg cggatgtggg caaggctgcc agggtaaaga caaataataa catcagagca
     8461 agcttgaact tcatggtttc ctcccgtaaa tcatacggaa acaaatacca gtataggcta
     8521 attacgtatg tatcccatag acgcaggcag ctgaggaaaa gttccagtgg tataatcctc
     8581 cagcaccagt ccggtgacaa gttattaaca aattaccgga tatcaaactc agataccaaa
     8641 acctagatct agcactgcta ttctgattaa ataattgaaa ttctatgctc gaaaaacttg
     8701 caggtattga atcaagatat caagaactaa accagcttct gaatgaagta ggcgacgact
     8761 accagcgcgc tgctgaactg aataaagaac gcattgactt agaacctctt tacagcaaag
     8821 cccaggagta ccgtaagtct ctggatcaac tggaacaggc ccgccatctc ctagataccg
     8881 atgacgagga attacgctcc ctggcagaaa ccgaattcac tgaactggaa gagctcatct
     8941 tccaattaga agatgaaatc aaagcgatgc tattgcccaa agaccggcgc gaccaacgta
     9001 acgtgattgt ggaaattcgc gcagggacag gtggcgagga agccgctcta tttgcctcag
     9061 acctcttccg catgtacagc cgctacgcag agcgccggcg gtgggatgtg gatatccttt
     9121 cggcgaacga gaccggcatt ggtggcttta aggaaatcat ctttatggtc aagggacgca
     9181 gtgcctactc tcgcttgaag tatgagtcgg gtgttcaccg tgtgcagcgt atcccggtca
     9241 ccgaggcttc aggacgcatc cacacctcga ccgccaccgt agcggtactg gcagaggtgg
     9301 acgaagtgga gatcgacatc cccgatagcg acattcgcat ggatgtgttc aagtcggctg
     9361 gggcgggcgg tcaaaacgtc cagaagaacg ccaccgccgt gcgcatcacg cacattccta
     9421 caggcattgt ggtcgcatgc caggatgagc gctcgcagct gcagaaccgg atgcgtgcca
     9481 tgagcatctt gcgcgcacgc ctgtacgaaa tcgaggaaga aaagcgccgc ctggaagttg
     9541 atgagacccg ccgttcccaa gtgggtactg gcgaacgcgc tgaaaaaatc cgcacctata
     9601 actaccccca gtcgcgggtc accgaccacc ggattaatat ctcaaacttc aacttaccaa
     9661 tgttcatcga tggggatatc gacgaattca tcgatgaact ggcaacccgt gacgaagcag
     9721 ctagactggc cgccgtaggg cttgagatag a
//
LOCUS       DBNGSX010000205         9165 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1816811, whole genome shotgun sequence.
ACCESSION   DBNGSX010000205 DBNGSX010000000
VERSION     DBNGSX010000205.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9165)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9165)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9165
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1816811"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="AC16CB_06280"
     CDS             <1..726
                     /locus_tag="AC16CB_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR3342109.1"
                     /translation="YRSPGELERTIFAIGSHDITLDVMAQFLAAKNRRLASANVGSQG
                     GLVALRRGEAHLAGSHLLDPETGEFNLRSVREYLPGVAVTVVALVGRQQGLLVPRGNP
                     KHIRALQDLARPDVTFVNRQRGAGTRVLLDYHLASLGSAAESVAGYRQEEYTHLAVAA
                     AVASGRADCGLGIAAAAQALDLDFLPLFQERYDLVIPQEYYQSELLAPVLDLLHQAEF
                     RQAIEALPGYDIEPMGAIIAQLD"
     gene            750..1211
                     /gene="bcp"
                     /locus_tag="AC16CB_06285"
     CDS             750..1211
                     /gene="bcp"
                     /locus_tag="AC16CB_06285"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006625139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HIR3342110.1"
                     /translation="MTLLKGTIAPDFTLLDETSTPRKLTDYRGQPVVLYFYPKDDTPG
                     CTTEACGFRDDYGDYQQAGVTILGVSPDSPKKHTKFKEKYNLPFNLLADTEHAVCELY
                     GVWGRKKFMGREYDGVFRTTYLIGADGVILQVFEKVKPDGHSAEILQALEK"
     gene            1477..3339
                     /locus_tag="AC16CB_06290"
     CDS             1477..3339
                     /locus_tag="AC16CB_06290"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3342111.1"
                     /translation="MNKWHELNRQKRHNLVRLGIAAAALILIIVYENLEWNWQIGTPR
                     GWWATLDAFLLALLGALAYFSVDRILRMVDERQALKEQLGHAESELNEMRQRQGTLLR
                     ISQMYLDASDEDEVVELALNLSRELLGARGASFVPLDENAQPLEARSLGEVPIADAGS
                     WLEYLASPAVREACDACQNREQLTHVCPLLKGAFQDATGVYCIPLRRREQDYGIFNLF
                     LPQSSVLDDEALLLLRTLMDETVIALENVRMRQRAMIALHQLQKLREKTDLNGLLADL
                     LVSLRDTLEADYVLVSIQDGESGPQKTEVSIGVDIPEKARHLINGILQSVLTSKEPVL
                     LESASGGMASAVGLHAMLAAPLVVQESRKDGPALGVILAASRRTRAFGQRQLSILQMI
                     AGQVSLVVQNVRLLAQLEYKTMIEERMRLAREIHDGLAQTLGFLKLKTAQMRSYLEQG
                     EGDLLQEAVDTCYSVLAEAYQDARQAIDGLRISSKEGFGEWLQQTVDEFQEHTGMLVD
                     LCDSQIAGALLPEVQVQLIRIVQEALNNVRKHAKARQVEIDCQRVGQDMVMEIRDDGG
                     GFNVEDVPGPSQHGLRGMRERAELIGADFQVISQPEKGTTVRIRLPVQERERLL"
     gene            3336..3977
                     /locus_tag="AC16CB_06295"
     CDS             3336..3977
                     /locus_tag="AC16CB_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3342112.1"
                     /translation="MKPISLLVVDDHALFRSGLISLLKGMPEFEVVGEAGNGQEALEA
                     ARQLAPDMVLLDVNMPVMDGVEAVRAIKAALTCRIMMLTISKNDDDLFGSILAGADGY
                     LLKNATPDELRKAILQVHGGMAVLAPDVTRKVMGAVRTDPASLASHGLSSREMEVLQC
                     LAQGLTTNQISNELFISDNTVKTHVRHIMEKLEASNRAEAVSKATQMGLIGGE"
     gene            4101..5543
                     /gene="coxB"
                     /locus_tag="AC16CB_06300"
     CDS             4101..5543
                     /gene="coxB"
                     /locus_tag="AC16CB_06300"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HIR3342113.1"
                     /translation="MTQRARPQRILIASSHALFGQGLRSLLLARQKAEVEVVGMVSSL
                     GEALAAIDVLNPDLVIVDYDDQALNREEFLARFVGSEKKLRVVLLSLQNPEEAIVYDR
                     RTMAAAQIDRWLEEWPTEEEAEAPPPPTGGGPLSANRLRRNTMKHLIIAGIFVVLVTV
                     LLIVGMDTAGLLPEQASLQAIPIDSMFDLEFKVIYFLFALIVVFMVYSIVVFRRKPGD
                     TSDARHVEGNTKLEVTWTLIPLVTVLTFSFLGAQSLAETQAIGDSVMEVNVIGSQWAW
                     RFEYPDTGIVSNELRLPVGKQALLHLQSTDVIHSFWVPEFRVKQDALPGGEEFIRDLR
                     VTPTKIGEYKVRCAELCGLNHAYMEAPVIVMAEADYKDWEIAESGLSPDPVVRGQKWY
                     ENYGCNACHTLDGTIKVGPSWKGLYGSQAKFTDGTTAVADDAYIYESIRNPGAKTVEG
                     FTPGLMPQNIAEKMSDDQISDVIEFIKTLK"
     gene            5591..7492
                     /locus_tag="AC16CB_06305"
     CDS             5591..7492
                     /locus_tag="AC16CB_06305"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HIR3342114.1"
                     /translation="MSENNRVNLFSTSLVRGLLAMVIGIPVGMGLVTVVRLLVGLPAW
                     KAEPAWVVGAVFGVVAFMLGTGVMNDWLKWARGEETPEHPIANPNLKGLAKYLSVSFD
                     HKVIGIQYGVTALILFTLAGAYALTFRTELLAPEIQFLQPKNFNTTMSLHGIVMIGAM
                     LIGVGAMSNYLVPLMIGANDMAFPRLNAFAYWVNVPAAVLLTLVWFLGGFDTGWTGYP
                     PLSAKAPLGMQMFFLGVYFIGFSSIFGSLNIIVTIVKMRAKGMSYFRMPIFVWTAFAT
                     ALIGLTATQLIGLSFQMVMFERLFGMGFFEPSKGGDPILFQHLFWFYSHPAVYVFVLT
                     GLGVISELLPVFVRKPLFGYRWVALSSFGIALVGFLVWAHHMFTSGMAAYLRVPFMYS
                     TLLVAVPTGIKFFSWVATMWEGKMSFETPMLFVLGAISIFLLGGLSGPPNGVVSTDLH
                     LQDTYWIVGHFHATMFGGFVFPFFAAIYYWFPKITGRMYNERLGKIHFYLMLPAFYVQ
                     SLGQMQVGLLGMRRRIADYDPALDINLSHQLITLAGFFIGISVLIAFYNFITSLRRGE
                     VAVANPWRSRSPEWQIPSPVPAHNFDQPFEVVGEPYDYGLEGSAYVSFTPSRPSTEVP
                     SGAEPAAAD"
     gene            7576..7803
                     /locus_tag="AC16CB_06310"
     CDS             7576..7803
                     /locus_tag="AC16CB_06310"
                     /inference="COORDINATES: protein motif:HMM:NF015582.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HIR3342115.1"
                     /translation="MDEKELEEKKLDAFRVGVGVLILLGVMTIGEYWLGAVASAWWSP
                     LLGVALLKAFFVVRDYMHLPRLFAPEEGAHE"
     gene            7800..8378
                     /locus_tag="AC16CB_06315"
     CDS             7800..8378
                     /locus_tag="AC16CB_06315"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIR3342116.1"
                     /translation="MTTHTSTLVEYRRKVAANRLGLWLFILSDTFVFGGLYVSRFYLL
                     GTQERPEVSQLLGFVVTAILLLSSYFMNRAETAMAHGDRKTFLRGTLTTLILGVIFLV
                     GVVGVEWQIAPFGPGDGAAASIFYTMTGFHAFHVLTGVIFLAVVYNNGRKGLYSAERH
                     WAVEACAVYWHFVDVVWIFFYPALYLIGLPVI"
     gene            8386..8985
                     /locus_tag="AC16CB_06320"
     CDS             8386..8985
                     /locus_tag="AC16CB_06320"
                     /inference="COORDINATES: protein motif:HMM:NF014667.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR3342117.1"
                     /translation="MRKNPLFYALYGLAALALITLGWRIFAGPYKFKGSQIDPPVPAA
                     DFTLTDQNSQAFRLSEQRGKVALIFFGYTNCPDVCPITLTTYTKIRARLGDEADKVRF
                     IFITVDPERDTVEQIRSHLGNFDPALIGLTGSREELESVWKNYGVFQSQHGAEGVEHY
                     DVDHSSRIYAIDPQGNWRLTYPAEMDYETIAKDVEHLID"
     gene            8985..>9165
                     /locus_tag="AC16CB_06325"
     CDS             8985..>9165
                     /locus_tag="AC16CB_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342118.1"
                     /translation="MNAKFDIGPYIIRFIVTALISVVVAGLINEGAYLFQKEKYDRPP
                     KTIQLVVPNGTADQLA"
BASE COUNT         1846 a         2395 c         2788 g         2136 t
ORIGIN      
        1 taccgctccc ctggcgaatt ggagcgcacc atcttcgcca ttggctccca cgacatcacc
       61 ctggatgtga tggcgcaatt cctggctgcg aaaaaccgcc gcctggcttc tgccaatgtg
      121 ggcagccagg gaggcttggt ggcattgcgc cgcggggagg ctcacctggc agggtcgcac
      181 ttgctcgatc ctgagacggg cgagttcaac ttgcgctctg ttcgtgagta tctgccgggg
      241 gtggcggtga ccgtggtagc cttggtggga aggcagcagg gcctgttggt gccgcgcggt
      301 aatcccaaac atatccgcgc cctacaagat ctagcccgtc ccgacgtaac ctttgtcaac
      361 cgccagcgcg gggcgggtac gcgggtgctg ctggattacc atctggcgtc gctcgggagt
      421 gcagcagagt ctgtcgctgg ctaccggcaa gaggagtaca cccatcttgc agtagccgct
      481 gcggttgcat cgggtcgcgc cgattgtggt ctgggcatcg ccgctgcggc tcaggcgctt
      541 gatctcgatt tcttgccttt attccaggag cgctacgacc tggttatccc gcaggaatac
      601 taccagagcg agttgcttgc ccctgtgctg gatttgctgc accaggcgga gttccgccag
      661 gcgatcgagg cgctgcctgg ctatgatatc gagccgatgg gtgctatcat cgcacaatta
      721 gattaaacgg ttatatcttt ggaggaccaa tgacactctt gaagggaaca attgcccccg
      781 actttacact attagatgaa accagcacgc cgcgcaagct tacagattat cgcggccagc
      841 cggtggtgct ctatttttat ccgaaggatg atacccccgg ttgcacgacc gaggcatgtg
      901 ggtttcgcga tgattatggg gattaccagc aagctggagt aacgattttg ggcgtgagtc
      961 cagattctcc gaagaagcac actaaattca aggaaaaata caacctgccc ttcaacttgc
     1021 tggctgatac cgagcatgca gtttgtgagt tgtacggggt ttgggggcgc aaaaagttta
     1081 tgggtcgtga atatgatggg gttttccgca ctacgtacct gatcggagcg gatggggtta
     1141 ttctccaggt attcgagaag gtcaaacccg atgggcattc cgcggaaatt ctgcaggcct
     1201 tggagaagta agcgctgcgg cagggtggca gcattgtgaa tcccggctct gctctgctgt
     1261 ggggtgctct cggtgctcag ctccaacacc cagggcaatt gcatatgccc tgaggggcat
     1321 gcgcaatccc gtaaaaagag tgtttggggt gttttggtgg gggcataccc ccactaaaac
     1381 accccatttt ctctcctcag tgatggggaa tcgcccaaat gcgattgtcc tgctccaaca
     1441 ccaaaaaggg ctttcgtttc gggtacaata gggcacgtga ataaatggca tgagctgaac
     1501 aggcaaaagc ggcataatct ggtgcgctta ggcatcgctg ccgctgcttt aattctcatc
     1561 atcgtctatg aaaacttgga atggaactgg caaattggta cgccgcgcgg ctggtgggcc
     1621 acgctggatg cgttcctgct ggcgctcttg ggagcgttag cctatttctc ggtagatcgc
     1681 atcttgcgca tggtggacga gcgccaggcg ctcaaggagc agctggggca cgcggaaagc
     1741 gagctgaacg agatgcgcca gcggcagggc acgctgttgc gtatcagcca gatgtacctg
     1801 gatgccagcg atgaagacga ggtcgtagaa ctggcgctga acttgtcacg cgaactgctc
     1861 ggcgctcgcg gtgcgtcttt cgtgccatta gatgaaaatg cacagccgtt ggaagccagg
     1921 agtctggggg aagttcccat tgcagatgcg ggaagctggt tggagtacct agcctcgccg
     1981 gctgtgcgtg aggcatgcga tgcatgccag aaccgtgagc agttgaccca tgtgtgcccc
     2041 ttgctcaagg gtgccttcca ggatgccacc ggggtatatt gcatccctct gcggcgccgg
     2101 gagcaggatt atggcatctt taatttattt ctgccccaaa gctcagtatt ggatgacgaa
     2161 gccctactac tgctccggac gcttatggat gagacagtca tcgccttaga gaatgtgcgc
     2221 atgcgccagc gagcgatgat tgccttgcac cagctgcaaa aattgcgtga aaaaaccgac
     2281 ctgaacgggt tgctggcaga tctgctggtg agcttgcggg atacgctgga agctgattat
     2341 gtgctggttt cgatacagga tggtgagagc ggaccccaaa aaacggaggt ctcgatcggg
     2401 gtggatatcc cggagaaagc ccgccacctg atcaacggga ttttgcaaag cgtgctgacc
     2461 tccaaagaac cggtcttgct ggagagcgcc tctgggggaa tggcatccgc ggtagggttg
     2521 cacgccatgc tggctgcgcc gctggtggtc caagaatcca ggaaagacgg gccagcgctg
     2581 ggggtcatcc tggctgccag ccggcgaacc agggcgttcg gtcagcgcca gctgtccatc
     2641 ctgcagatga tcgccgggca ggtctccctg gtggtgcaga acgtgcgcct gctggcccag
     2701 cttgagtata agacgatgat cgaagagcgc atgcgcctgg cgcgggagat ccatgatggt
     2761 ctggcgcaaa ccctgggatt cttgaagctg aagaccgcac agatgcgctc gtatctcgaa
     2821 cagggtgaag gcgatttgct ccaagaagcg gtagatacct gttatagcgt gctggcagaa
     2881 gcctaccagg acgctcgcca ggcgattgac gggctgcgca tctcttccaa ggagggcttt
     2941 ggagagtggt tgcagcagac ggtcgacgag ttccaggagc acactggcat gcttgtcgat
     3001 ctgtgcgact cgcagattgc tggtgctctt ctgcctgagg tccaggtgca gttgatccgc
     3061 atcgtccagg aggccctgaa caacgtgcgc aagcatgcca aagcccggca ggtggagatc
     3121 gactgccagc gtgtgggcca agacatggtg atggagatcc gcgacgacgg cggggggttt
     3181 aatgtggagg atgtgcctgg tccatcgcag cacgggttgc gcgggatgcg cgagcgggct
     3241 gagctgatcg gcgctgattt ccaggtgatc agccaacctg agaaggggac aaccgtccgc
     3301 atccggctgc cagtgcagga gagggagagg ctgttatgaa accgatcagc ttgctggtgg
     3361 tggatgatca tgccctgttc cgctcggggt tgatcagcct cttgaagggg atgccagagt
     3421 tcgaggtggt gggcgaggca ggcaacggcc aggaagccct ggaggccgcc cgccagcttg
     3481 cccctgatat ggtcttgctg gatgtcaaca tgccagtcat ggatggggtg gaggcggtgc
     3541 gcgccatcaa agcggccctt acttgccgca tcatgatgct gaccatctcc aaaaatgacg
     3601 atgacctgtt tggctcgatc ctggctggcg cggatggcta cctgctcaaa aacgccaccc
     3661 cggacgagct tcgcaaagcc atcctccagg tgcatggtgg catggcggtg ctggcgccag
     3721 atgtaacccg caaggtgatg ggcgcggtgc gcaccgaccc ggcgagcctt gccagccacg
     3781 ggttgagcag ccgggagatg gaagtgttgc agtgcctggc acagggcttg accaccaacc
     3841 agatatccaa tgagctgttt atctcggata acaccgtcaa aacccacgtg cggcacatca
     3901 tggaaaagct ggaagcttcc aaccgggcgg aggcggtcag caaagccacc cagatgggtt
     3961 tgatcggggg agagtagaag cgcaagttgt atcttcggag gggtgatgaa aaaatcaccc
     4021 ctccgggtta gttaaatccc tcttttatga caagggtggt ggatgacatt ttagaccttc
     4081 tttggtaaat tataggaggt atgacacagc gcgccagacc ccagcgcatt ttgatcgcct
     4141 cttcccacgc tctctttggg caggggttgc gtagtctgtt gcttgcccgc cagaaagcag
     4201 aggtcgaagt agtgggtatg gtttccagcc tcggcgaggc gctggcagct atcgacgtgc
     4261 tcaaccccga cctggtcatc gtggattacg acgaccaggc cctcaaccgg gaggagtttt
     4321 tggcgcgttt tgtgggcagt gagaagaaat tacgcgtggt cttgctctcg ctacaaaacc
     4381 cagaagaggc catcgtctat gaccgccgca ccatggcggc cgcccaaatt gaccgctggc
     4441 ttgaggagtg gcccaccgaa gaagaagcgg aagcgccgcc gccccccact gggggtgggc
     4501 ctctttccgc caaccgtctc aggagaaaca ctatgaaaca tttgatcatt gctggtattt
     4561 tcgtcgtcct tgtcacggtt ttgctgatcg tagggatgga cacggccggg ctcttacccg
     4621 aacaggcaag cctgcaagcc atccccatcg actcgatgtt tgacctggaa ttcaaggtga
     4681 tctacttcct gttcgccttg attgtggtgt tcatggtcta cagcatcgtg gtattccggc
     4741 gtaagcccgg agatacatct gatgcccgcc atgtggaggg caatacgaag ctcgaagtga
     4801 cctggaccct catcccgctg gtgaccgtgc tgactttttc cttcttggga gcccagtctc
     4861 tggctgagac ccaggcgatt ggggatagcg tcatggaggt gaacgtgatt ggttcgcagt
     4921 gggcctggcg ctttgaatac cccgatacgg gaatcgtatc caatgaattg cgcttgccgg
     4981 taggcaagca ggcacttctg cacctgcaat caacggatgt gatccattcc ttctgggtgc
     5041 ctgagttccg cgtcaagcag gacgccctgc cgggtggaga ggagttcatc cgcgatctgc
     5101 gcgttactcc gaccaaaatt ggcgagtaca aggtgcgctg cgccgaattg tgcggattga
     5161 atcacgctta catggaagcg ccagtgatcg ttatggcaga agctgactac aaagattggg
     5221 agatcgccga atcaggtctc tcgccagacc ctgtggtgcg cggccaaaag tggtacgaaa
     5281 actacggctg caatgcctgc catacgttgg acggaaccat caaggttggt ccgagttgga
     5341 agggtctcta tggaagccag gctaagttca ccgatgggac aacggctgtc gcggatgacg
     5401 cttatattta cgaatccatt cgcaatccgg gcgctaagac ggtggagggt ttcacacctg
     5461 gacttatgcc gcagaatatc gccgagaaga tgagcgacga tcaaatcagc gatgtgatcg
     5521 agttcatcaa gaccttgaaa tgataatgag atacccgcaa tgacggcaca atcatataag
     5581 gagtttgggt atgagtgaaa acaacagagt taatcttttc tccaccagcc tggtgcgtgg
     5641 cttgctggcg atggttattg gtattccagt aggcatgggc ttggttaccg tggtgcgcct
     5701 gctggttggc ttacccgctt ggaaggccga accggcttgg gtggtgggtg cggtgtttgg
     5761 cgtggtagct ttcatgctgg gcacgggcgt gatgaatgac tggcttaagt gggcgagagg
     5821 ggaagagaca cccgaacacc ctatagccaa tccaaatctg aagggtctgg ccaagtactt
     5881 gagtgtctcg tttgatcaca aggtgatcgg cattcaatac ggggtcactg cgctgatcct
     5941 gttcacgtta gcgggtgcct atgccctcac attccgcacc gagctgcttg ccccggagat
     6001 ccaattcctg cagccaaaga attttaacac caccatgagc ttgcatggca tcgtgatgat
     6061 cggagctatg ctgatcggcg taggggcgat gtcgaactac cttgtgccgt tgatgattgg
     6121 cgccaacgat atggctttcc cacgcctgaa cgccttcgct tactgggtca acgttccggc
     6181 ggctgtcctg cttaccctgg tgtggttcct gggcggtttt gatacaggct ggacgggcta
     6241 cccgccgctg agtgccaaag cgcctcttgg catgcagatg ttctttttgg gcgtgtattt
     6301 tatcggtttt tcttcgatct ttgggtcgct taatatcatc gttaccatcg tcaagatgcg
     6361 cgccaagggc atgagctact tccgcatgcc gatcttcgtt tggacagcct tcgccacagc
     6421 tctcatcggc ctgaccgcga cccagctcat cggcctgtct ttccagatgg tcatgtttga
     6481 gcgcctgttt ggtatgggtt tcttcgagcc gtccaaaggt ggcgacccga tcctgttcca
     6541 gcacctgttc tggttctact cgcacccggc ggtgtacgtc ttcgtgctca ccgggttggg
     6601 ggtgatcagc gagctgctgc cggtctttgt gcgcaaacca ttgtttggct atcgctgggt
     6661 ggcgctgtcc tccttcggta ttgccctggt tggattcttg gtctgggcgc accatatgtt
     6721 cacctcgggc atggcggctt acctgcgtgt gcccttcatg tatagcaccc tgctggtggc
     6781 tgtgccgacg ggtattaaat tcttcagctg ggtggctacc atgtgggaag gcaagatgag
     6841 cttcgagacc cccatgctgt tcgtgctggg cgccatctcg atcttcctgc tgggtgggct
     6901 gagcggcccg cctaacggtg tggtctccac cgacctgcac ctgcaagaca catactggat
     6961 cgttgggcac ttccatgcca ccatgtttgg cgggtttgtc ttccccttct ttgcagccat
     7021 atattactgg tttccaaaaa tcactgggcg catgtacaac gagcggctgg gcaagatcca
     7081 tttctacctg atgctgccgg ctttctatgt gcagtcgttg gggcagatgc aggttggcct
     7141 gctgggtatg cgccgccgca ttgcagatta cgatccggcc ttggatatta acctgtcgca
     7201 ccagttgatc accctggcag gtttctttat tggtatctcg gtgttgatcg ccttctacaa
     7261 cttcatcacc agcctgcgcc ggggtgaggt ggcggttgcc aacccgtggc gctcgcgctc
     7321 acccgaatgg cagatcccct cgccagtgcc agcgcacaat ttcgaccaac ctttcgaggt
     7381 ggtcggagag ccgtacgact atggtttgga aggctcagcg tatgtcagtt ttaccccatc
     7441 ccgcccctcc acggaggtgc cgtctggggc agagccagcc gcggctgatt gagctggagt
     7501 gtagagatcc ttgactgaag gctgagcatc agcccttcat cacgaaatgt tgtggagtat
     7561 ttcaggagtt aaatcatgga cgaaaaagaa ttggaagaga agaaactaga cgcttttcgc
     7621 gtgggcgtgg gtgtgctcat cctgttgggc gtgatgacca tcggcgagta ctggcttggc
     7681 gcggtggctt ctgcctggtg gtcgccactg ctgggcgtgg ctctgctgaa agcctttttt
     7741 gtggtgcgcg attatatgca cctgccgcga ctctttgccc ctgaggaggg tgcgcacgaa
     7801 tgacaacaca tacttcaaca cttgtcgaat atcgccgcaa agtggcagct aaccgcctgg
     7861 gattgtggct gttcatcctt tcggatactt ttgtatttgg tggactgtac gtcagtcgct
     7921 tctacctgct tggcacgcaa gagcgccctg aagtcagcca gctactgggt tttgtagtca
     7981 ccgccatact tcttctgagc agctatttca tgaaccgggc tgagaccgcc atggcgcacg
     8041 gggatcgaaa gaccttcttg cgtggcacgc tgacgacact gattctgggt gtgatctttc
     8101 tggtcggcgt ggtgggagtg gagtggcaga ttgccccctt tggccctggt gatggagcgg
     8161 ccgcctcgat cttttacacc atgacgggtt tccacgcttt tcacgtgcta accggcgtga
     8221 tcttcctggc tgtggtgtac aacaatgggc gcaaggggct ctactcagcc gagcgccatt
     8281 gggcggtgga agcctgcgcc gtgtactggc actttgtcga tgtggtgtgg attttcttct
     8341 acccggcttt gtacctgatt gggttgccgg ttatttgaga gcgaagtgag aaagaaccca
     8401 cttttttacg cattgtatgg actggctgcg ctggcgctga tcacgcttgg ctggcgcatt
     8461 ttcgctggcc cgtacaagtt caagggctcg cagatcgacc cgcctgtgcc agccgcagac
     8521 ttcacactga cggatcaaaa cagtcaggca ttccggttga gcgagcagcg tgggaaagtg
     8581 gcgttgatct tctttgggta caccaattgc ccggatgtct gcccaatcac gctcacgaca
     8641 tataccaaaa tacgcgctcg cttgggcgat gaggcagaca aagtgcgctt tatctttatc
     8701 accgtagatc ctgaacgtga tacggtagag cagatccgct cgcacctggg gaattttgac
     8761 ccggcgctta tcggtctgac cggcagccgg gaagaacttg agagcgtctg gaagaattac
     8821 ggcgtgttcc agtcccagca tggagccgag ggggtggagc attatgatgt ggatcactct
     8881 tcgcgcatat atgccatcga cccacaaggt aactggcgat tgacatatcc ggccgagatg
     8941 gattacgaga ccatcgccaa ggatgtggag catttgattg attgatgaac gcaaagtttg
     9001 atattggtcc ctatatcatc cgctttattg tcaccgcgct gatctcggtg gtagttgctg
     9061 gacttataaa tgagggagct tacttgttcc agaaagagaa atatgaccgg ccgccaaaga
     9121 ccatccagct cgtcgtcccc aatgggacgg cagaccagtt ggctg
//
LOCUS       DBNGSX010000206         3118 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1886408, whole genome shotgun sequence.
ACCESSION   DBNGSX010000206 DBNGSX010000000
VERSION     DBNGSX010000206.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1886408"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /gene="thrC"
                     /locus_tag="AC16CB_06330"
     CDS             complement(<1..1214)
                     /gene="thrC"
                     /locus_tag="AC16CB_06330"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR3342119.1"
                     /translation="MKKKFLGYQCSLCNTQYAPDEVTYTCPKDGGNLDVILDYEAIKR
                     ETTPEKIAAGEESSLWRYLPLLPIADPQGLGTPLRAAGGTPLFAPKQLAENLGLRSLW
                     VKDESRNPTASFKDRASAVVVARAREVGAEVVVTASTGNAGAALAGMAAAVGQQAVIF
                     APKTAPPAKIAQLLIYGARVLLVDGTYDDAFDLTIQAAQEFGWYCRNTGYNPFTAEGK
                     KTAALEIWEWVKIPRATIFVSVGDGNIISGIHKGFKDLLELGWMENMPRIFGVQAEGS
                     AAVANAFHARTETITPVSARTLADSISVDLPRDGVRAVRAARDTGGSYIVVSDEDIIS
                     SIAALGQVGIFAEPAGATSHAGLVKAIGLGLIDPDDPVIVINTGSGLKDVKAAMQAAG
                     QAPIIEPTLEAV"
     gene            complement(1319..2887)
                     /locus_tag="AC16CB_06335"
     CDS             complement(1319..2887)
                     /locus_tag="AC16CB_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3342120.1"
                     /translation="MMSDTDTLPSGHPRITHLELRGIVKRFPGVLANDHVNFDVRAGE
                     VHALLGENGAGKSTLMKILYGLYQPNEGEIWLNGSKVTISSPSDAIRLGIGMIHQHFM
                     LVPSLTVAENVALGLPSSRGPITDLDLVSIRINELAKVYGLQVDPSAYVWQLSVGQQQ
                     RVEIIKSLYRGAALLILDEPTAVLTPQEVDDLFVTLNQMAKDGHAMIFISHKLHEVLS
                     ISDRVTVLRDGRVVGTQPTSSTTKGELANMMVGRPVSLKIDHQQVELGQVRLELNDIW
                     AMSDRGTEALRGVNLEVRSGEILGLAGVSGNGQRELAEVIAGLRPATRGKVMLDSQVV
                     SGRSPGELIEYGLSYIPEERMRDGMIREFTVSENLILREHHKMPYSQAGFLRPPDITK
                     ISAELIEDFNVKTPSQETPVKNLSGGNIQKLILARELSRKPGVVIAAQPTRGLDIAAT
                     EYVHYRLMEQRREGTATLLISEDLDEILALSDRIAVIYEGKIMGVVPAAQAEPKELGL
                     MMAGVMAEAKGQEN"
BASE COUNT          712 a          913 c          760 g          733 t
ORIGIN      
        1 ctgaccgcct cgagggtagg ctctataatc ggggcttgac cagcggcttg catggcagct
       61 ttgacatctt tcaaaccgct accggtgtta atcacgatta ctggatcatc cggatcaatc
      121 agccccaagc caatcgcctt gaccaatcca gcatgtgacg tagctccagc aggctcagca
      181 aaaataccta cctgacctaa ggccgcgata gatgagatga tatcctcgtc gctgacgaca
      241 atatacgatc cgcccgtatc gcgagcggcg cgcacggcgc gcacaccatc tcgcggcagg
      301 tctacagaga tgctatcggc aagtgtgcgg gcagaaaccg gggtgatcgt ctccgtcctg
      361 gcatggaaag cattcgcaac ggcggcagaa ccttcagcct gcactccaaa aatgcgaggc
      421 atgttctcca tccagcccag ctcgagcagg tccttaaagc ctttgtggat gccagagatg
      481 atgttcccat cgccgacaga gacaaatatg gttgctcgag gaatttttac ccactcccag
      541 atttccaacg cagccgtctt cttgccctcc gcggtgaacg gattgtatcc cgtgtttcga
      601 caataccagc caaactcctg tgcagcttga atagtcaggt caaaggcgtc atcgtatgtg
      661 ccatccacca acaataccct ggccccgtat atcagaagct gggcaatctt ggcagggggg
      721 gccgtctttg gagcgaaaat aaccgcctgc tgcccaactg cggcggccat acccgccagg
      781 gctgccccgg cattgcctgt cgaggcggtg accactactt cagccccgac ctcacgagcg
      841 cgcgctacaa ccacagcact cgctctatcc ttaaatgagg ctgttgggtt acggctctcg
      901 tccttcaccc acagggagcg caatcccaga ttttctgcca gctgcttggg agcgaagagc
      961 ggcgtaccac cagccgccct cagcggtgtc cccaatccct gagggtcagc gataggcaga
     1021 aggggcaggt agcgccatag cgagctttcc tcaccagccg caattttttc cggggtggtc
     1081 tctcgcttta ttgcctcata gtccaggatg acatccaggt taccaccatc tttggggcaa
     1141 gtgtaggtga cctcatccgg cgcgtattgt gtattacata aggagcattg ataaccaagg
     1201 aattttttct tcattttatt aaccacagaa atcacacagt tgggcagcta atgacggtta
     1261 taggcagatt tcgcccgcca gcaacagcct cttttgcttc aactgtgtaa gcctcagact
     1321 aattttcttg accttttgct tctgccatca ccccagccat catcaagccc agctctttgg
     1381 gctcagcctg tgctgcaggt acaactccca taatcttgcc ttcatagata accgcaattc
     1441 gatcagagag cgccaggatc tcatccaaat cctcagatat taataacgtt gcggtgcctt
     1501 ctctgcgctg ctccatcagc cggtagtgca catattccgt agctgcgata tccaatcctc
     1561 gcgttggctg ggcagcgatg actacgccgg gctttctgga aagctctctg gctaagataa
     1621 gcttttgtat gttaccgcca gatagattct tgaccggggt ctcctgggaa ggggttttaa
     1681 cattgaaatc ttcgatcagc tcagcagaga ttttcgtaat atccggcggc ctcaagaaac
     1741 ccgcctgcga gtaaggcatt ttatggtgtt ctcgcaatat cagattctca gagacagtga
     1801 actcccggat cattccatcc cgcatgcgct cctcagggat atatgataaa ccgtactcaa
     1861 tcaactcgcc cggcgatcga ccggatacaa cctggctatc tagcatcacc ttcccacgtg
     1921 ttgctgggcg caagcctgca atgacctccg ccagctccct ctgaccattt ccagataccc
     1981 cagccagccc gaggatttcg cctgatcgca cttcaagatt gacccctctg agtgcctccg
     2041 ttccccgatc gctcatcgcc cagatatcgt ttagctccaa gcgcacctgt ccaagctcaa
     2101 cctgctgatg atcgattttc aggctgacag gtcgcccaac catcatattg gctagctcac
     2161 ctttcgtggt agaggatgtg ggttgtgtgc ccacaacccg cccatctcgc aacacggtga
     2221 cccggtcact aatacttaaa acttcgtgca atttatgaga aataaagatc attgcatgac
     2281 cgtctttagc catctggttc agggtgacga acaaatcgtc gacttcctgc ggggtgagca
     2341 ccgcggtggg ctcatccagg atgagcagcg ctgcgccccg ataaagggac ttgatgatct
     2401 ccactcgctg ctgctgcccc accgaaagct gccaaacata agcgctcgga tcgacttgca
     2461 aaccgtatac tttggccagc tcgttgatcc tgatcgagac aagatcgaga tccgtgatcg
     2521 ggccccgcga ggatggtaaa cccaaggcga cattttccgc aaccgtcagg gagggaacca
     2581 acataaagtg ctggtggatc atcccaattc ccaggcggat ggcatctgag ggcgaggaga
     2641 tggtcacctt gctgccatta agccagatct ccccctcatt aggctggtaa aggccgtaga
     2701 ggattttcat cagggtactt ttgcccgctc cattctcacc aagcagtgcg tgaacttccc
     2761 cggcgcggac gtcaaaattt acatggtcgt ttgccagcac gcctgggaag cgctttacaa
     2821 tgccgcgcaa ctctaagtgt gttatgcggg gatgaccgga gggcaaagta tctgtatcac
     2881 tcatcatatt ttggttaatt atctctatag tgaatttatt gggtgaagga gagagcgatc
     2941 attataatcg ctctctccaa tagttatact acctgaacgt tagtaccgag actactcgct
     3001 cggaccttcc atgtcctcca gcagctgggg caggtaccaa atttcttcat cggttgccac
     3061 agcgccggct gcgacgtatt ctgtgccatc ctggagcgtg accggccccg tccagaca
//
LOCUS       DBNGSX010000207         5837 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1470934, whole genome shotgun sequence.
ACCESSION   DBNGSX010000207 DBNGSX010000000
VERSION     DBNGSX010000207.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5837)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5837)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5837
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1470934"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="AC16CB_06340"
     CDS             complement(<1..907)
                     /locus_tag="AC16CB_06340"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3342121.1"
                     /translation="MKSDLDSLMLSNQIDALLITGPAMHNPPMTYFTGGVHVTGGDLI
                     KKRGEAPVLFYHDMERDEAARTGLATKSLDAYRFPELLKKSNGNWVQATILRYQKMLT
                     DAGLTSGRIALYGKIDAGEAYAIFSGLQGTMPALEFVGQTNDSLLLKARTTKDSDEVQ
                     RIRHMGQITVEVVGLTADFLTSHRAKNGLLVKPDGQPLTIGEVKRRINLWLVERGAEN
                     PYGSIFAIGYDSAVPHSSGTSSDPLHLGETIIFDIYPCETGGGYFYDLTRTWCLGHAT
                     DEAQALYEDVLAVYTQVLSELKPGIH"
     gene            complement(922..2271)
                     /gene="selA"
                     /locus_tag="AC16CB_06345"
     CDS             complement(922..2271)
                     /gene="selA"
                     /locus_tag="AC16CB_06345"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HIR3342122.1"
                     /translation="MSDLRSLPSVEQLLQTPQLKDLSAAYGRPLALEALRFVLDEARA
                     RFPDLGSVPNREALLAHTQELLEKWTAPTLLPVINASGVILHTNLGRAPLSDAALRAA
                     NSVSKNYSNLEYDLQRGRRGSRLLHAEALLCRLTSAEAALVVNNNAAAVLLALTALAR
                     RRAVVIARSQLVEIGGGFRVPDVMKQSGARLLEVGTTNRVHLSDYEAALEERPAMFLH
                     AHRSNFRILGFTSEPALDEIARLAHQASVPLVDDLGSGSLLDTARYGLGHEPTVQESL
                     QGGADLVCFSGDKLLGGPQAGIIAGRADLVSKLKKHPLARAVRADKLCLAALTATLLH
                     YVKDEAERQIPVWRMIAARPEEIKARAQSWIEHLGRGEIIPGESTVGGGSLPGETLPT
                     WLLALDVPSPNRLLGRLRQCQLPIIARTQDDRVVLDPRTVLSEQDSVLLDELGVELA"
     gene            complement(2334..2738)
                     /locus_tag="AC16CB_06350"
     CDS             complement(2334..2738)
                     /locus_tag="AC16CB_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342123.1"
                     /translation="MDTSSSPTAKSVLPAAITLAVIGWGGLGAVVFFTLPTVGPRWLF
                     FFLGVLALTGSALPFVAYLNRRFPSTPPVNSGVILRQSVWVGIYAATLAWLQIGRVLT
                     PSLALVLALGFVIIEWLIRLREKSQWKPETPE"
     gene            complement(2810..3301)
                     /locus_tag="AC16CB_06355"
     CDS             complement(2810..3301)
                     /locus_tag="AC16CB_06355"
                     /inference="COORDINATES: protein motif:HMM:NF017015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3342124.1"
                     /translation="MFSKEMIQTLFGYHFWANDRILDYAARLEPADYFADAGYSHGSL
                     HGILFHILRTDYAWRISCQTLKPAVEPLLLENFPDLQAIRSRWEEEQQAMLAYLSGLS
                     PDEIEGAIQLTDRRGNLHQFKLWRVLVHVILHAMQHHSEAAALLTQYGHSPGDLDFIF
                     YRG"
     gene            complement(3302..5032)
                     /locus_tag="AC16CB_06360"
     CDS             complement(3302..5032)
                     /locus_tag="AC16CB_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012239.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HIR3342125.1"
                     /translation="MRILNEKQEALLANERGLLNDLRVALVQFGAASEDHETLGQSIR
                     QLDELFLLVVVGEFNSGKSAFINALLGQKVLQEGVTPTTTQINILRYAEAPGRLYDNE
                     NVQTLTAPVDMLTEISIVDTPGTNAIIREHEAITTQFVPRSDLVLFITSADRPFTESE
                     RLFLQRIRDWGKKVVVVLNKIDLFQSDDELAQVQSFIAENARTLFGRTPEIFPVSARL
                     ALRAKMGEPSLWATSRFEPLERYIHDTLDEKGRLHLKFLSPLGVGLHLSEKYLGVIDS
                     RLDLLKDDFTTLEDVEAQLSVYKEDMQRDFNYRMSDIEKVLFEMEQRGQDYFDETLRL
                     GRVFDLVNKSKIQREFEGHVVGDTPQRIETKTGEIIDWLVDSDLRQWQAVNEHLAERR
                     RQHAERIVGDSAGGTFHHDRERLIEGVGREARRVVDSYDKTQEAQAIAEGARNAVAAL
                     AAVEVGALGLGTLVTVLATTATADVTGVLLASLMAALGLFLIPAKKRQAKLEMRTKIG
                     AMREQLARSLRVQFEREIERSLGNIHEAIAPYTRFVRAEQGKLQGAQQELEKIKTDLG
                     KLKVEVEAGD"
     gene            complement(5089..>5837)
                     /locus_tag="AC16CB_06365"
     CDS             complement(5089..>5837)
                     /locus_tag="AC16CB_06365"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR3342126.1"
                     /translation="IIGGGVRGGGVSSMPTPESARSWYPASDPVHGFDHVLRVYHLAE
                     QLAQAEGADLEIVRAAALLHDATGDQRSVDSHGLPYAQAGGQPSEISNQHSTINYIRL
                     NHHHASSDFAAQVLRAEGWDEARIAAVQHCIRAHRFRDNRESPHTLEAKILFDADKLD
                     AIGTVGVARAIGYAIQAGQPAYAKPSQQFLDSGELEPGEPHSAYHEFLFKLRHLKDRL
                     YTPAAKSIANERHRVMAEFFERLAGEMGGK"
BASE COUNT         1219 a         1677 c         1704 g         1237 t
ORIGIN      
        1 aatggatgcc aggcttgagc tcgctcaaaa cttgcgtata gacagcgagc acatcttcgt
       61 aaagcgcctg cgcttcatcg gttgcatgcc ccaggcacca ggtgcgggtg agatcataaa
      121 aatagccgcc tcccgtctca cagggataga tatcaaatat gatggtctca cccaggtgca
      181 acgggtcgct gcttgtaccg ctgctgtggg gcaccgcgga gtcatatccg atggcgaata
      241 tggagccata tgggttttca gcgccacgct caaccagcca taaatttatg cggcgcttga
      301 cctcaccgat cgtgagcggc tgcccatcgg gcttgaccag caggccgttt ttcgccctgt
      361 gggaggtgag aaaatcggca gtgagcccta ccacctcaac agtaatttgt cccatatgcc
      421 gaatgcgctg cacttcatcg gaatcttttg tggtgcgagc tttgagcagc agggaatcgt
      481 tggtctggcc gacaaactct agtgctggca tagtcccctg caaaccggag aaaatggcgt
      541 atgcctcccc ggcgtcgatt ttgccgtata gggcgatccg cccagacgtg aggcccgcat
      601 cggtgagcat cttctggtag cgcaggatgg tagcctgcac ccaattacca ttgctctttt
      661 ttagcagctc ggggaagcgg taggcatcca ggctttttgt cgccaacccg gtgcgtgcag
      721 cttcgtcgcg ttccatgtcg tggtaaaaca acacaggcgc ttccccgcgc tttttgatca
      781 agtcaccacc tgtaacatga accccgccag tgaaatacgt cattgggggg ttgtgcatcg
      841 ccggccctgt gatcagcagg gcgtcgatct ggttggagag catcagggag tcaaggtcag
      901 atttcattag agttattcac ctcaagccaa ttctacccct aactcgtcaa gcaagacact
      961 atcttgctct gagagcacgg ttctcggatc caggacaacg cggtcatcct gagtgcgtgc
     1021 gatgatgggt aactggcact ggcgcaggcg tcctaataag cggttggggc tgggcacatc
     1081 cagggcaagt agccaggtag gcagtgtctc gccgggcagg ctgcccccgc ccacggtaga
     1141 ttcgcctgga atgatctcgc cgcgtcccag atgctcaatc cagctttggg cgcgcgcctt
     1201 gatctcttcc gggcgagctg cgatcatgcg ccagacggga atctggcgct cagcttcatc
     1261 tttcacatag tgtagcaagg tggcggtaag cgccgccagg caaagcttat cggcgcgcac
     1321 ggcgcgcgct aaggggtgtt tctttagttt ggagaccaga tcagcgcgcc cggcgataat
     1381 cccagcctgc ggaccgccca gcaatttatc gcctgaaaag cacaccaggt ctgcgccccc
     1441 ctgcagcgat tcctgaaccg tgggctcatg tcccagcccg tagcgagccg tgtccagcag
     1501 cgagccagaa cccaggtcgt ctaccagagg gacgctagct tggtgtgcca ggcgcgcgat
     1561 ctcgtccagg gcgggctcac tggtaaaacc caggatacgg aaatttgagc ggtgagcgtg
     1621 caggaacatg gcagggcgct cttccagggc agcttcataa tcggagagat gaactcggtt
     1681 ggtcgtgccc acttctaaga gcctggctcc ggactgtttc attacgtcgg gaacgcggaa
     1741 gccgccgcca atctccacca gctgagatcg agcaatcacc acggcacgcc ggcgagcaag
     1801 agcggtgagg gctaacagca cggctgctgc attgttatta accaccaggg cagcttccgc
     1861 actggtcagc cggcacagca atgcctcggc atgcaacagg cgcgatccgc ggcgaccacg
     1921 ctgcaagtcg tactccaggt tggaataatt tttggatact gaatttgcgg ctcgcagggc
     1981 ggcgtcgctg agcggggcgc gaccgaggtt tgtgtgcaga atcacgccag aggcattgat
     2041 cactggcagc agggttgggg cggtccactt ctccagcaac tcttgggtat gggcaagcaa
     2101 cgcctctcgg ttgggcacag agcccaggtc ggggaagcgc gctcgcgcct cgtccaggac
     2161 aaaacgcaac gcctccagag ccagcggtcg cccgtaggca gcgctcagat cttttagctg
     2221 gggggtttgc aggagttgct cgaccgaagg cagggagcgt aaatcggaca tgggataatt
     2281 gagaagggtt acagagagtt tggtgtagcc accctttcag gtcgcctacc tgactactct
     2341 ggggtctctg gcttccactg gcttttctcg cgcaagcgga ttagccactc gatgatcaca
     2401 aagccaagtg ccagcacaag agccagcgaa ggtgtgagca cgcgcccaat ctgcagccat
     2461 gccaaggtgg ctgcatagat gccaacccag actgactggc gcaaaatcac tccagaatta
     2521 acaggaggcg tgctggggaa acgccggttt aaataagcta caaaaggcaa agcgctgccg
     2581 gtgagtgcga ggacacccag gaagaagaat agccagcgcg ggccgacggt gggcaatgtg
     2641 aagaacacca ctgcccccag tccaccccac ccgatcacag ccagagtgat ggcggcgggt
     2701 aagacagatt ttgcggttgg ggaagaggaa gtatccaaat cttttctcgg taagactagt
     2761 tttatctgct tattttatct tatctcacac aaaggtcacc agcgatcttc tagccacggt
     2821 aaaagataaa atcgaggtcg cccggcgaat gcccatattg ggttagcaag gcggcagctt
     2881 cggagtgatg ctgcatggca tgcaaaatga catgtaccag cactcgccaa agcttaaact
     2941 ggtgcaggtt gccgcgccgg tccgtgagct ggatagcgcc ttcgatctcg tctgggctga
     3001 ggccggagag ataagcgagc atggcttgct gctcttcttc ccagcggctg cggatagctt
     3061 gcaggtcggg gaaattttcc aacaaaagtg gctcgacagc cggtttgagt gtctggcagc
     3121 tgatccgcca ggcataatcg gtgcgcagaa tgtgaaaaag aatgccgtgc aagctgccat
     3181 ggctgtagcc cgcgtcagcg aaataatcgg ctggctccag cctggcggcg taatctaaga
     3241 tgcgatcatt ggcccagaag tggtaaccaa atagcgtttg gatcatctcc ttggaaaaca
     3301 tttaatctcc cgcctctacc tccaccttca atttgcctaa gtcggtcttg atcttctcca
     3361 actcctgttg ggcaccctgc agcttgccct gctcagcgcg cacaaagcgg gtataagggg
     3421 cgatggcttc atggatattg cccaggctgc gctcgatctc gcgctcaaac tgcactcgca
     3481 aggagcgagc aagttgttcg cgcatggcgc cgatcttggt gcgcatctcc agcttggcct
     3541 gacgcttttt ggcagggatc aagaacaagc ccagcgcagc catcaagctg gccaataaca
     3601 cgccagtcac atcggcggta gccgtggtcg ccaggaccgt caccagcgtc cccaacccaa
     3661 gggcacccac ctccacggct gccagggcag ccaccgcgtt gcgtgccccc tcggcaatgg
     3721 cctgggcttc ttgggtctta tcgtagctgt ccacaacccg gcgcgcctcg cggcctaccc
     3781 cttcgatcaa acgctctcgg tcgtggtgga acgtgccccc cgcagagtct cccacaatgc
     3841 gctcggcatg ctggcgccgg cgctcggcca gatgctcgtt caccgcctgc cactggcgca
     3901 gatcggaatc aaccaaccaa tcgatgatct ccccggtttt ggtctcgatg cgctgggggg
     3961 tgtcgcctac cacatgcccc tcgaattcac gttggatctt gctcttattg actaaatcga
     4021 agacacgccc caggcgtagt gtctcgtcga aataatcctg gccgcgctgt tccatctcga
     4081 agagcacctt ctcgatgtca gacatgcgat agttgaaatc gcgctgcatg tcttccttat
     4141 agaccgaaag ttgagcttcc acgtcttcta aggtggtgaa gtcatctttg agcaggtcca
     4201 gccgggagtc gatcacgccc agatatttct ctgacaagtg cagccccacg cccagtgggc
     4261 tgaggaactt caggtgcagg cggcccttct cgtcgagtgt atcgtgaatg tagcgctcta
     4321 gcggctcaaa tcggctggta gcccacaggg agggctcgcc catcttggcg cgcagtgcca
     4381 ggcgggcgct gactgggaag atctccggtg tccggccaaa taaggtgcgg gcattttcgg
     4441 ctatgaagct ctgcacctgc gccagctcat catcgctttg gaataaatcg atcttattaa
     4501 gcaccactac aaccttcttt ccccaatcgc gaatgcgctg caggaaaagt cgctcgcttt
     4561 cggtgaaggg tcgatcggcc gaggtgatga ataggaccaa atcggagcgc ggcacaaact
     4621 gggtggttat ggcttcatgc tcgcggatga tggcgttggt gcccggcgtg tccacaatgc
     4681 tgatctcagt cagcatatcc acgggagcgg tcagggtttg cacgttctca ttgtcgtaca
     4741 agcgcccagg cgcctcggcg tagcgcagta tattgatctg cgtggtggtc ggcgtaacac
     4801 cttcctggag cactttttgc cctagcagag cgttgatgaa agcgctcttg cccgagttga
     4861 actcccctac caccaccagc aggaatagct catcgagctg gcgaatggac tgccccagtg
     4921 tctcgtggtc ttcggatgcc gccccaaact ggacgagggc gacgcgcagg tcgttgagca
     4981 ggccgcgctc attcgctagt agggcttctt gtttttcgtt gaggatgcgc atgtgtgata
     5041 ttccctggca aaaattatag cattagtaag gggagaatcg attttgcctt acttaccccc
     5101 catctcccct gccagccgct cgaaaaattc cgccatgacc cggtggcgct cattcgcgat
     5161 ggacttcgcc gcaggcgtat acagccggtc tttcagatgg cgtaacttga agagaaactc
     5221 gtggtaagcg ctgtgcggct cgccaggctc cagttccccg ctatccagga actgctggga
     5281 tggcttggcg taggcaggct gaccggcttg gatggcgtaa ccaatggcgc gcgccacacc
     5341 caccgtgccg atggcgtcca gcttatcagc gtcgaaaagg atcttggctt ccagggtgtg
     5401 tggcgactcg cgattgtcgc ggaagcggtg agcgcggatg cagtgttgta cggcggcgat
     5461 acgcgcttca tcccagcctt ctgcccgtaa cacctgcgcg gcgaagtcgg atgaagcgtg
     5521 gtgatgattc aatcttatat agttgattgt ggaatgttga ttgctgattt ctgacggctg
     5581 accacctgcc tgggcgtacg gcaggccatg gctgtctact gaccgctgat ctcctgttgc
     5641 atcatgtagc agcgctgctg cgcgcacaat ctccaggtcg gcgccttcgg cctgggctaa
     5701 ttgctccgcc aggtggtaga cgcgcagcac gtgatcgaac ccgtgcacgg ggtcagaggc
     5761 ggggtaccag gagcgggcgg attcaggagt aggcatagaa ctaacccccc cacccctgac
     5821 ccctccccca ataatgg
//
LOCUS       DBNGSX010000208         2675 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1263226, whole genome shotgun sequence.
ACCESSION   DBNGSX010000208 DBNGSX010000000
VERSION     DBNGSX010000208.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1263226"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..510)
                     /locus_tag="AC16CB_06370"
     CDS             complement(79..510)
                     /locus_tag="AC16CB_06370"
                     /inference="COORDINATES: protein motif:HMM:NF013486.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HIR3342127.1"
                     /translation="MTYPRFSDAEFQRRYRLARELMHREGLSALVVFGNSGINRHNEA
                     NVFWLTNYLDLHHNYLVFPLHGKPTLLTGLVNHVPNARAIAVINDTRWGRYEPAKEIA
                     AQLVKTGIGKDHVGLVGVNATFGMGMPYRHYATLKEALPET"
     gene            complement(507..1565)
                     /locus_tag="AC16CB_06375"
     CDS             complement(507..1565)
                     /locus_tag="AC16CB_06375"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3342128.1"
                     /translation="MPDERVEAAIKNWAPRFTSQGVDYNDFFRTTARIQKWEEWCREW
                     CVTGDVHHDLAVKAEAQGNSLSAGEAYISAALCYHFGKFVFQDFHDEYMSASQKAVEA
                     FAKGLKLLDPTGERLEIPLDKIRMVGTLRRPPESDKPPLVILLPGLDSTKEEFFHWER
                     VFLKRGMATFTLEGPGQGECGYNSHIRPDYEVAVSATLDILTKRGDLDNSRIGLAGVS
                     LGGYYAPRAAAFEPRLKAAVGNCGPWNFGECWDNLPSLTRSAFLYHSGASDMEDAKAR
                     AHQLSLDGVAQKIKQPLLVIHGKLDRLIPWQHAHQIVDAVGPNAQLAMFENGNHVCNN
                     IPYIYRPLTADWLKEKLG"
     gene            complement(1558..2580)
                     /locus_tag="AC16CB_06380"
     CDS             complement(1558..2580)
                     /locus_tag="AC16CB_06380"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3342129.1"
                     /translation="MSEPVGVTVIGTGMWGKHMLAAVKRTPSMRLIACYSRDVAAREA
                     VAAEFGCAAPETFEQAINWDGVQGVLLITPNDVHAEQALECAQWGKHIFVEKPIAYSL
                     EDGIAMHSACKSAGVTLFVGHGFRRSGAARKVKQMLDEGRLGQVVLAEANFSLPGTLT
                     PDKWRYYRQTCPGGPLMQLGIHHADTLQYWLGPVKRVRGTFAHLATSAEIDDVGIAQL
                     EFSNGALGTLTGSYVSPKTYYLRLYGTEGVLDYQTDMRIWPNADQMDAATILTYRTKS
                     AVEQLKFEPRDMLVDELDEFARCIQGSAQPETGALEGLRALDVILSSIRSHETGTPIA
                     LGEYNA"
BASE COUNT          612 a          765 c          689 g          609 t
ORIGIN      
        1 caacggacgc gctccacggc ccgctgccag ccggcgtaga gggcgcaggc gggcaaattc
       61 gcgggtcacg tcaacccatt aggtttcggg gagcgcttct ttcaaagtcg cgtaatgtcg
      121 ataaggcatc cccatcccaa aggtcgcgtt gacgcctacc aacccgacat gatctttacc
      181 gatgcctgtc ttaaccagct gcgcagcgat ctctttggca ggttcatatc ttccccagcg
      241 cgtgtcgtta atcacggcaa tcgcgcgcgc attgggcaca tggttcacca gacccgtgag
      301 aagagttggc ttaccatgca gcggaaagac cagatagttg tggtgcaggt cgaggtaatt
      361 tgtcaaccaa aagacattgg cctcattatg acggttgatg ccagagtttc caaacaccac
      421 cagagcagat agaccctcac ggtgcatcag ctcgcgggca agacggtatc gcctctggaa
      481 ctccgcatcc gagaagcggg gataagtcat ccaagcttct ccttgagcca gtcagcggtc
      541 aacgggcggt aaatataggg aatattattg caaacgtgat tcccgttctc gaacatagcc
      601 agctgcgcgt tagggcccac agcatccacg atctggtgag catgctgcca ggggatcaaa
      661 cgatcaagtt tgccatggat gaccaatagc ggttgtttga tcttttgcgc aaccccatcc
      721 agggatagct gatgtgcgcg ggcctttgca tcttccatgt cactcgctcc tgagtgatac
      781 agaaaggcag accgtgtgag cgatggcaga ttatcccaac attccccaaa gttccaggga
      841 ccacaattgc ctaccgcagc tttgaggcgt ggctcaaagg ctgccgcgcg cggagcgtaa
      901 tatccaccca agctgactcc cgccagtccg atgcggctgt tatccaaatc gcctcgcttt
      961 gtcaatatat ccagcgtagc cgagacagcc acctcgtaat cagggcggat atgcgagttg
     1021 tagccgcact cgccctgtcc aggaccttcc agggtgaagg tcgccatgcc gcgtttcagg
     1081 aagacccgct cccagtggaa gaattcttct ttggtggaat caagcccggg caggagaatc
     1141 acgagtggtg gcttatccga ctctggaggt cggcgcaagg tgcctaccat ccttattttg
     1201 tctaacggga tctccaaacg ttcccctgtt gggtcgagca atttcaggcc tttggcgaaa
     1261 gcctccaccg ctttttgcga agcggacata tattcatcat ggaaatcctg aaagacgaac
     1321 ttgccaaaat ggtaacaaag cgccgcagag atataggcct cgcctgcgct gagagaatta
     1381 ccctgcgctt cagcttttac ggcgaggtca tgatgcacat cacccgtcac gcaccactcg
     1441 cggcaccatt cctcccattt ctggatgcgg gcggtggtgc ggaaaaagtc gttataatcc
     1501 acaccctgag aagtaaagcg cggagcccag ttcttgatgg cagcttcaac tcgttcatca
     1561 ggcattgtat tctcctagtg caataggggt tcccgtttca tgagagcgga tagatgacaa
     1621 aattacatcc agcgctcgca gtccttccaa tgcgcccgtc tcaggctggg ctgaaccctg
     1681 gatgcaacgg gcaaattcat ctaattcatc caccagcatg tcacgcggct caaatttcaa
     1741 ctgctctaca gcagatttgg tcctgtaagt caaaatggtg gctgcatcca tctggtcagc
     1801 atttggccag attctcatat ctgtttggta atcgagcact ccctccgtcc cgtaaaggcg
     1861 caggtagtat gttttgggag atacgtaact tcctgtcaac gtgccaagcg caccgttgga
     1921 aaattcgagt tgtgcaatcc caacatcgtc aatctcagcg gaggttgcca agtgggcaaa
     1981 ggtgccacgg acacgcttga ctggtcccag ccagtactgc aaggtatcag catggtggat
     2041 gcccagttgc atcagcgggc cgcctggaca agtttggcga taatagcgcc atttgtcggg
     2101 cgtgagggta cctggcaagg aaaagttcgc ttctgccagc acaacctgtc ccagcctgcc
     2161 ttcatccaac atttgcttca ccttgcgtgc ggcacccgaa cgccgaaagc catgccctac
     2221 aaacagtgtt acacctgccg atttgcacgc cgaatgcatc gctatgccat cttccaacga
     2281 gtacgcgatg ggtttctcga caaagatatg ctttccccat tgagcgcact ctagagcctg
     2341 ctccgcatgg acatcattgg gcgtgatcag cagcacacct tggactccat cccagttgat
     2401 agcctgctcg aatgtctctg gcgcagcgca gccgaactca gctgctacag cttctcgggc
     2461 agccacatcg cggctgtaac aggctatcag gcgcatagat ggggttcgct tgacagccgc
     2521 cagcatgtgt tttccccaca tccccgtgcc gattacggtc acgccgactg gttcgctcat
     2581 acctctatca tcctggtcat ggtcaatcgg caccagcata aacccctgca gtttcagggt
     2641 aaatggtttt tatgatctta aagcggtgga tatcc
//
LOCUS       DBNGSX010000209         6981 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_708948, whole genome shotgun sequence.
ACCESSION   DBNGSX010000209 DBNGSX010000000
VERSION     DBNGSX010000209.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6981)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6981)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6981
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_708948"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="AC16CB_06385"
     CDS             complement(<1..206)
                     /locus_tag="AC16CB_06385"
                     /inference="COORDINATES: protein motif:HMM:NF024924.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3342130.1"
                     /translation="MSEPSTQTVTINTDLARRIQDELGQNVYLCYQCVKCTSGCPVGE
                     FFDWQPNQVMRALQLGQEDIALES"
     gene            complement(266..1297)
                     /locus_tag="AC16CB_06390"
     CDS             complement(266..1297)
                     /locus_tag="AC16CB_06390"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - L-methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HIR3342131.1"
                     /translation="MTADEKSNGYLVGSNLERVLRAGHFAVTGELGPPQSADGEVIRK
                     KAALLRGYVDAVNITDNQTAIVRMSSIGAGAILVQEGLEPVIQMTCRDRNRLAIQSDI
                     LGAYALGMRNLLCLSGDHQTFGNHPTAKNVFDIDSIQLLHLVTAMRDEKIFECGDAFK
                     GQEPRFFTGAAAAPFADPIDFRPYRLAKKVNAGANFIQTQLVYDVPAFARYMEKVRSL
                     GVHKQTYILAGVGPLKSPGMARYMKKDVPGILVPDEVIERMEGAGAPWTGKKADELSK
                     EDKKERSTAWKNEGIQICIELIQALRQIEGVAGVHIMAIEWEEAVKPIVEGAGLYPRP
                     VVEVEAAPL"
     gene            complement(1357..2040)
                     /locus_tag="AC16CB_06395"
     CDS             complement(1357..2040)
                     /locus_tag="AC16CB_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HIR3342132.1"
                     /translation="MIVAEQKSLEELKTLVSGAEKVLVVGCGTCVTVCFAGGAREAAI
                     AASSLRMASKLEGNPQEVTDVTVQRQCEWEYLDQIAEQVKDADIVLSLGCGVGVQAIA
                     EHFDQTWVVPGLNTKFLGLPTEQGVWAERCAACGDCVLGLTGGICPIARCSKSLLNGP
                     CGGSEAGHCEINPEIPCAWQLIYDRLNSLGKLNVLLEFQPPKNWRTSRDGGPRKIVRE
                     DLRLVMERE"
     gene            complement(2059..2625)
                     /locus_tag="AC16CB_06400"
     CDS             complement(2059..2625)
                     /locus_tag="AC16CB_06400"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3342133.1"
                     /translation="MLPEQIKTYREIVTLRDGANVLLRPMTLEDEKGMIELFTPVSDE
                     DLRYLRDNVRDPSVIREWCDKLDYAKVLPLLAVVKERIVGEATLHFRKGPRRHIGEVR
                     IYLAKDFRQRGLGTKMLKTLIELARKQDLHILLAEIVTEQTQVIKAFQNLGFVLRCTF
                     DDFFMLPDGDLRDAAILTLALRPRTYDF"
     gene            complement(2709..3125)
                     /locus_tag="AC16CB_06405"
     CDS             complement(2709..3125)
                     /locus_tag="AC16CB_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(2925..2927),aa:Sec)
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HIR3342134.1"
                     /translation="MGDTSLQIIAYCCEHCAYAAADLAGGLRMQYPPEVKVVQLPCTG
                     KLDVLMVLQAIEDGADGVLVAGULPGDCHYLEGNVNARRRVERIKELLTQIGLEPERV
                     RMENMSAAMAGKFVSVAKEMTEQIATLGPNPLKNAD"
     gene            complement(3184..>6981)
                     /locus_tag="AC16CB_06410"
     CDS             complement(3184..>6981)
                     /locus_tag="AC16CB_06410"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3342135.1"
                     /translation="GFGVTVFEAMPVAGGMLRLGVPEYRLPTEIIEREVQDIIDLGVD
                     LRLNHQVNNLDEVFEQGYEAVLIAVGAHEGIRLPIPGADLQGVLVNTHFLRDVRLGVY
                     SENGKNPSARRSDLGKKVLVLGGGNVAIDCARTAVRMGCEVHLTCLESREQMPAHAWE
                     VEAAEAEGVILHPGRAFERILDDGQGRAGGVECTRVSSFSFDERGRLLVEKVADSNHI
                     LDCDTVIFSVGQRAGLAFIPDDANVGMTERQTIAINPNTLATSRPGVFAAGDCISGTA
                     FVIEAVDRGHTAAQSIIRYLQGEALEPLAKPELPVVRLSQGEIAERMARGEIHPRPRV
                     PMPELTVEQRRDNFAEVEGGYDDASAQDEAARCLACGICSECMSCVFACGAKAIEHDM
                     VAHTESVRVGAVILAPGYQVYQAELSEEYGYGRYPNVVTSLQFERLLSASGPTMGHVQ
                     RPSDGKAPRRVAFLQCVGSRDQTHDYCSAVCCMYATKEAVIAKEHDPDLEVHVYMMDM
                     RAFSKGYWGYFERAQDRYGIQYHRCRVSALREDPDTHELYLQVAEETSDGLRPITERF
                     DMVVLSVGMEISPAVRELGRRIGIEMDEYGFCHTVQFNPVETSRPGMYAVGPFREPKD
                     IPESVVEASGAAGAAAVDISAARFTLTERREIPPERDVGEESPRIGVFVCHCGSNIAG
                     FLDVPQVTQYAASLPGVAHAEANLYTCSQDSIRHITEVVKENGLNRVVVASCTPLTHA
                     PLFQDSIRAAGLNPYLFEMANIRNQCSWVHSREWDVATGKAKDLVRMAVARAALLQPQ
                     HTIELSVQRAALVVGGGVAGMTTALSLAGQGFPVHLVEKEAELGGNLRQVYTAAPSLN
                     GGKTPEGPQEILRRLETQVRADKLITLHLSSQVASSSGFMGNFNSTIQGPDGQRQEIT
                     HGATILATGASEYRGPEYGYGSHPRIVTQMELESLFDNQQATIDNLKSVVMIQCVGPG
                     ENFCSRICCTVALKNALALKEKNPGLQVTILYRDIRTYGFKERLYTEARRKGVLFLRY
                     DDQHKPQVQIGEAADQDLAVSAEQPIAVKVRDAALGRDIELHPDLVVLSMPVVPNPQV
                     HELSALFKVPADADGFFLEAHVKLRPVDFSTDGIFMAGMAHYPKLLDESIIQAQAAAA
                     RAARVLSREALIAGGRVAVVDETRCTGCLTCVRICPFNVPKIQANLSGVGNILGAAYI
                     EAAVCQGCGQCAAECPARAIQLMHYTDAQMAAKVRALVELDAGFVPLEEITIGSIRPG
                     SDN"
BASE COUNT         1365 a         2177 c         1907 g         1532 t
ORIGIN      
        1 tgtgactcta gggcgatgtc ctcctgaccc aattgcaggg cgcgcatgac ctggttaggc
       61 tgccagtcga agaactctcc caccgggcag ccgctggtac atttgacaca ctggtagcac
      121 aggtacacgt tctgccccag ctcatcttgg atgcgccgcg ccagatcggt attgatggtt
      181 acggtctgtg ttgatggttc actcataggc tattcctttt ggcccctttt tgaggagccg
      241 cccctggatt gccgatactt cttgctcata gcggtgcagc ctctacttca actacagggc
      301 gcgggtacaa tcctgccccc tccacgatag gcttgaccgc ctcttcccac tcaatggcca
      361 tgatgtgcac cccggccaca ccctcaattt gacgcagcgc ctggatcagc tcaatacaga
      421 tctgaatgcc ctcattcttc caggctgtag agcgctcttt cttgtcttcc ttgctcagtt
      481 cgtcggcttt cttgcccgtc cagggagccc ctgccccctc catgcgctcg atgacctcgt
      541 cgggcacaag gatgccgggt acatcttttt tcatatagcg cgccatgccc gggctcttca
      601 gcggtcctac tcctgccagg atgtaggtct gcttatgcac ccccaaggag cgcactttct
      661 ccatgtagcg ggcaaaggct ggcacatcgt acaccagttg agtctggatg aaattcgcgc
      721 ctgcattgac cttttttgcc agccggtagg gacggaaatc tatcgggtcg gcgaagggcg
      781 ccgcggcagc gccggtgaag aagcgcggct cctggccttt gaaggcgtcg ccgcactcaa
      841 aaatcttctc gtcgcgcatg gcggtcacaa gatgcaacaa ctggattgaa tcgatgtcga
      901 agacattctt ggcggtggga tggttgccga aggtctggtg gtcgccgctc aggcagagca
      961 agttgcgcat ccccagggcg taggcaccca gtatgtcgct ttgaattgcc aggcgattcc
     1021 ggtcgcggca ggtcatctga ataacaggct ccaatccttc ttgcaccagg atggcacccg
     1081 cgccaatgct ggacatgcgc acaatggcag tctgattatc ggtgatgttg actgcgtcga
     1141 cgtatccgcg cagcagcgcc gcctttttac ggatcacctc gccatcggcg ctctgtgggg
     1201 gtcccaactc tcccgtgaca gcgaaatgcc ctgcccgcag cacccgctcg aggttagatc
     1261 cgaccaggta tccattgctt ttctcatcgg ctgtcatcgc tcatcctcct gttgggatac
     1321 ataagaagtg atctgccaca agtcacacat cacgttctac tccctttcca ttaccaggcg
     1381 caagtcttcg cgcacaatct tgcgcggtcc gccatcccgg gaagtgcgcc aatttttagg
     1441 cggctggaat tccagcagca cgttcagctt gcccaggctg ttcaaccggt cgtagattaa
     1501 ttgccaagca caagggatct ctggattgat ttcgcagtgc ccggcttccg agcctccaca
     1561 gggtccattg agcagcgatt tcgaacagcg ggcgataggg caaatgccgc ctgtcaagcc
     1621 aagaacacag tcgccgcagg ccgcacaacg ctcagcccac acgccttgct cggtgggcag
     1681 gcccaagaac ttggtgttca atccgggcac tacccaggtt tggtcgaaat gctcggcgat
     1741 agcctgcacg cccaccccgc agcccaagga gagcacaatg tcggcatcct ttacctgctc
     1801 ggcgatctgg tctagatatt cccattcgca ctggcgctgt accgtcacat cggtgacctc
     1861 ctggggattg ccttccagct tgcttgccat gcgcaaggat gaagctgcaa tagctgcttc
     1921 acgcgccccg ccagcaaagc acacggttac gcacgtgcca cagcctacca ccagcacttt
     1981 ttcggcacca ctcaccaggg tttttagttc ttccagagat ttttgttcgg caacgatcat
     2041 agatctaccc tccaatcatt aaaaatcgta cgttcttggc cgcagagcca gggtcaggat
     2101 ggccgcgtcg cgcaaatcgc catccggtag catgaaaaag tcgtcaaaag tacagcgcag
     2161 gacgaagcct aaattctgaa aggctttgat cacctgggtt tgctcagtca cgatttctgc
     2221 tagcaaaatg tgcaggtcct gcttgcgtgc cagctcgatg agagttttga gcatttttgt
     2281 gcccaggccg cgctggcgga aatctttggc gagatagata cgaacttcgc caatatgcct
     2341 tcgtggtccc ttgcgaaagt gtagcgttgc ctctccgacg atgcgttctt tgacaacagc
     2401 cagcagcggc aacaccttgg cataatccaa tttatcgcac cattcccgga tgacgctcgg
     2461 gtcgcgcacg ttatcgcgca ggtagcgcag gtcttcatcg ctgaccgggg tgaacagctc
     2521 gatcatgcct ttctcgtcct ccaaggtcat cgggcgcagc agaacgttgg caccatccct
     2581 cagggtgaca atctcgcgat aggttttaat ttgttccggc agcattcttc ctcctgatct
     2641 cagattgaga ccctagattg acgatcaagg cttaaaggag tcaccattca atcattaatc
     2701 gacaattctc aatctgcgtt ttttaacgga tttggcccca gcgtcgcaat ttgctccgtc
     2761 atctcttttg ccacgctgac aaacttaccg gccatggctg ccgacatgtt ctccatgcgc
     2821 acgcgttccg gttctaggcc gatctgggtc agcagttcct taatgcgctc tacccggcgc
     2881 cgggcgttta cattaccttc caggtaatgg cagtcgccgg gcagtcagcc ggctaccagc
     2941 acgccatcag cgccatcctc tattgcctgg agcaccatca gcacgtccag tttgccagtg
     3001 cagggcagct gaaccacttt cacctctggc gggtactgca tgcgcaaacc acccgctagg
     3061 tcagcggcgg cgtaagcgca atgttcacag cagtatgcga tgatttgcaa cgatgtatct
     3121 cccatccgag agtccttctc ctgaaaataa ccggtaaatg actaataacc cggataccaa
     3181 atcctaattg tccgatcccg gtcttatgct cccaatggtt atctcctcta agggcacaaa
     3241 accggcgtcc agttcaacca gggctctcac cttggctgcc atctgggcgt ctgtgtaatg
     3301 catcagttga atggcccgcg ccggacattc cgcggcgcac tgcccgcaac cctggcacac
     3361 tgcagcttcg atatatgctg cgcccagtat gttccctacg ccgctcaggt tggcctggat
     3421 ctttggcaca ttgaatgggc agatgcgcac gcaggtcagg cagcccgtac agcgcgtctc
     3481 gtccaccact gccacacgcc ctccagcgat caatgcctcg cggctgagca cgcgcgcggc
     3541 acgcgcggca gcagcctggg cttgaatgat gctctcatcg agcagcttgg gatagtgcgc
     3601 catgccagcc ataaagatgc cgtcggtgga aaagtccacc gggcgtagct tgacatgcgc
     3661 ttccaagaag aagccatctg catcggctgg caccttgaag agcgccgaga gctcgtgcac
     3721 ctgcgggttg ggcacaaccg gcatggagag cacgaccagg tctgggtgga gctcgatgtc
     3781 acgtcccagc gctgcatctc gaactttgac cgctatgggt tgttcggcgc tgactgctaa
     3841 gtcttgatcg gctgcctcgc caatttgaac ctgtggcttg tgctgatcat cgtagcgcaa
     3901 gaaaagcacg ccttttcgcc gcgcctcggt gtatagccgt tccttgaatc cataggtgcg
     3961 aatatcgcgg tacaggatgg tcacctgcaa gcccgggttt ttctccttga gcgccagggc
     4021 gttcttgagg gccactgtgc agcaaatccg gctgcagaaa ttctcccctg gccccacgca
     4081 ttggatcatc acgacggatt tcaggttgtc aatggtggct tgctgattgt cgaataatga
     4141 ctccagttcc atctgcgtta cgatgcgcgg atggcttcca tatccatatt ctgggccgcg
     4201 gtactcgctc gccccggttg ccaggatggt ggcgccatgg gtgatctcct ggcgctgccc
     4261 atccggtccc tgaatggtac tgttgaagtt gcccatgaac ccgctgctgg aagccacttg
     4321 gctgcttaag tgcagcgtga tcagcttgtc tgcccgtacc tgcgtttcca ggcggcgcag
     4381 gatctcttgc ggaccttcgg gcgttttccc gccgtttaac gatggcgctg cggtgtatac
     4441 ctgccgcaag ttgccgccta gctcggcttc cttttccacc agatgcaccg ggaagccctg
     4501 acctgccagg gataacgcag tggtcatacc tgccacaccg ccgccgacca ccagtgctgc
     4561 ccgctgcacg gacaactcaa tggtatgctg cggctgcagt aatgcggctc gcgccactgc
     4621 catacgcacc aggtctttgg ctttgccggt agccacatcc cattcacgcg agtgcaccca
     4681 agagcactgg ttgcggatgt tagccatctc aaataaatat gggttcagcc ccgccgcgcg
     4741 gatgctgtcc tggaagagcg gtgcatgggt cagcggcgta cagctcgcta cgaccacacg
     4801 attcagcccg ttttctttga ccacctcggt gatgtgccgg atgctatctt gcgagcaggt
     4861 gtacaggttg gcctcggcat gcgctacacc gggtaacgaa gcggcatatt gggttacttg
     4921 gggcacgtcc aggaaaccgg cgatgttgga accgcagtgg cagacaaata cgccaatgcg
     4981 aggtgactcc tcacccacat ctcgctcagg tgggatttcg cggcgctccg tcaaagtgaa
     5041 gcgcgccgcg ctgatatcca ccgctgccgc tcctgctgcc ccgctggcct ccaccaccga
     5101 ttcggggata tccttgggct cgcggaaggg accaacggca tacatgcctg gccggctggt
     5161 ctccaccggg ttgaactgta ctgtgtggca gaacccgtat tcatccattt caatgccgat
     5221 gcgccgcccc aattcgcgca ctgcgggaga aatctccatg cccaccgata gcactaccat
     5281 gtcaaagcgc tcagtgatgg ggcgcaaacc gtcgcttgtt tcctccgcga cttgcaaata
     5341 cagctcgtgg gtatcggggt cctcgcgcag cgcgctgacc cggcagcgat ggtattggat
     5401 gccgtagcga tcctgtgccc gttcgaaata tccccaatag cccttcgaga aggcgcgcat
     5461 gtccatcata taaacatgga cttccaaatc ggggtcgtgc tccttggcaa ttaccgcctc
     5521 tttggtggca tacatgcagc agaccgccga acagtagtcg tgagtctggt cccggctgcc
     5581 cacgcattgc aggaaggcga cgcgtcgagg cgctttgcca tcggaaggac gttgtacatg
     5641 ccccatggtt ggccctgagg ccgagagcag gcgctcgaac tgcagggagg tgaccacatt
     5701 gggatagcgc ccataaccgt actcttcaga tagctcggct tgatacacct ggtagcccgg
     5761 ggccaggatg accgccccca cccgcactga ctctgtatgg gcgaccatgt cgtgttcgat
     5821 ggccttggct ccacaggcga acacgcagct catgcactcc gaacagatgc cgcatgccag
     5881 gcagcgggct gcttcatcct gggcgctggc gtcatcgtac ccgccttcta cctcggcgaa
     5941 gttatctcgg cgctgctcta cagtcagttc gggcatgggc acccgcggcc ggggatggat
     6001 ctctcctcgc gccatccgct cggcaatctc tccctgagag aggcgcacaa ccggcagctc
     6061 cggtttagca agcggctcca gcgcttcgcc ttgcagatag cggatgatcg attgggctgc
     6121 agtgtgaccg cggtctaccg cctcgatcac gaaggctgtg cccgagatgc agtcgcctgc
     6181 ggcgaacaca cccgggcggg aggttgctaa tgtattggga ttgatggcga tggtttgccg
     6241 ctcggtcatg cccacattgg catcgtcggg gataaacgcc aagcctgcgc gctgccctac
     6301 ggagaagatc accgtatcgc agtcgaggat atgattgcta tctgccacct tctccaccag
     6361 caggcggccg cgctcgtcga agctaaacga tgagacccgc gtgcactcca cgccaccagc
     6421 gcgaccttgc ccgtcgtcca gaatgcgctc gaaggcgcgc cccgggtgca ggatgacacc
     6481 ctccgcctcg gcagcttcca cctcccaggc gtgggcgggc atttgctcgc gactctccag
     6541 gcaggtcagg tgcacctcgc aacccatgcg cacggcggtg cgggcgcagt cgatggctac
     6601 gttgccaccg cccaacacca acaccttttt tcctaaatca gaacgacgag cagatgggtt
     6661 ctttccgttt tctgaataga cccccagcct gacatccctg aggaagtgcg tgttgaccag
     6721 cacgccctgc agatcggccc cggggatagg caggcggatg ccctcgtgcg cccctacggc
     6781 gatcaagact gcctcgtaac cctgctcgaa gacttcatcc aggttgttga cctggtggtt
     6841 caagcgcaag tcaacgccca aatcgatgat atcctgcacc tcacgctcga tgatctcggt
     6901 aggcaggcgg tattcgggca cgcccaggcg caacatgccg ccagcaaccg gcatggcctc
     6961 gaacacggtg accccgaagc c
//
LOCUS       DBNGSX010000210         8817 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_570462, whole genome shotgun sequence.
ACCESSION   DBNGSX010000210 DBNGSX010000000
VERSION     DBNGSX010000210.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8817)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8817)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8817
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_570462"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..681
                     /locus_tag="AC16CB_06415"
     CDS             70..681
                     /locus_tag="AC16CB_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342136.1"
                     /translation="MTRTYQNISAPAIEDDREIRQNLGAFDRNKAEAYQEAAIKDSFI
                     RLPERETQDAWTDAVPEIQGVASQECLEAAGADHVRSFNWTPKYLNHNWSLLLQPIYT
                     TYYLDDENQPQLVYVHGQSAKISGVRRASMKRAQRLALMILGAAIFMAFLSLVITAIG
                     VVAPPLLVIGGLGLGASVFVGLGALLPLVIAWQFNRNQKGMAK"
     gene            686..1534
                     /locus_tag="AC16CB_06420"
     CDS             686..1534
                     /locus_tag="AC16CB_06420"
                     /inference="COORDINATES: protein motif:HMM:NF015690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HIR3342137.1"
                     /translation="MAFEDKPLITAFVSDLYFSSRIESVTQRMGFTVLFIERDEEDEP
                     NNLGDVDIQYAEHLDGQGGALLDKLTQQLPALIIFDLGNADITWRKWILLIKSSPATR
                     RTPVLCFGSHMDTESMKAAKAAGADAVLARSKFVQSLSELIQKYARRIDYGALKDACQ
                     HSLSEIAVRGLEEFNRGEYFEAHESLEDAWNQDQTAGRELYRAILQVAVAYLQIERRN
                     FRGAMKMFLRLRQWIDPLPDMCRGVNVAQLRTDALEVHEALKALGPEHINEFDHSLFA
                     PVLYFR"
     gene            1614..2417
                     /locus_tag="AC16CB_06425"
     CDS             1614..2417
                     /locus_tag="AC16CB_06425"
                     /inference="COORDINATES: protein motif:HMM:NF027889.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp1/GlmU family protein"
                     /protein_id="HIR3342138.1"
                     /translation="MVVGAPDTGKSTFARYLFKRLSGEGLRVAYLDGDPGQSTLGPPT
                     TLSLVIAHPGERRFPPAGQIWRWFIGSVSPHGHMLPLLVAAARLVRVGLEGGADVVVY
                     DTCGLIDPNQGGMALKLAKIDLLQPQGLFAIQISDELEPLLIPMRRSKRARVVDLSSS
                     SAVIPRVQAARQAYRAEQFAGYFRKAGRVDISWSSQPVFPAPRFALNRLVSLDDANGF
                     ALGLGIVLNVNRQMQSVELLTPLPSLEGVQSITLGDISLDRETFRDSMI"
     gene            2460..2969
                     /locus_tag="AC16CB_06430"
     CDS             2460..2969
                     /locus_tag="AC16CB_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR3342139.1"
                     /translation="MEYFDLIQKRYSVRAYKPDPVEDDKLQKVLEAARLAPTAANRQP
                     FQIIVIHTEGRKDELMKIYRRDWFVQAPLLICAVGVPRTSWVRADRRRYLDVDVAIVM
                     DHLILAATSLGLGTCWVAAFNEEMARKSLHLPDEVEPLVFTPLGYSADQPTEKERKEI
                     SQLIRFEHW"
     gene            3197..5434
                     /locus_tag="AC16CB_06435"
     CDS             3197..5434
                     /locus_tag="AC16CB_06435"
                     /inference="COORDINATES: protein motif:HMM:NF026177.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3342140.1"
                     /translation="MNKRILLLLADVKAEKSLSKYFQTRGDLVWHTNDVSNVLGLVKR
                     YKPEIVVIDLHMPGENWLEALSTIRKYRPSAKIIITNKYPDFRRELRAKEYGVKVFLR
                     QPFKQKWLDKAIQAVEIGEQLPASKRTILPRVRVSMQVKITFPYALLALFFALASAFL
                     ISRYVLESLRDRFLIQLIDTGSLTADWMVQEENRLLETLRLIANSEGVPDALLAGDVE
                     RLQTIALPVAVNSQEEAIEILYASGEIMLSLHHRPGGTVEDYTVVQGDPQLAQQVFIR
                     QVLYGRVDDQGDKFAGLARLQRGDYFYIAGPIYDTEGRLVGAVAVGESLSKMVQSIRR
                     DTLAHVSIYSMDGNPIISTIFMEENSRPLPVEIVAKILKSQDSESPVREMSISNTNFS
                     EILGPWEVRGGHDLGIIGTTLRQNFITRPSFITRFQVIAIILVGVSGVILLGIYLAHQ
                     ITSPLSKVVKASMEVAKGNFEVKVPSQGNDEVMVLAHAFNYMVSGLQEGFIYRDLLGR
                     TVSPEVREALRESFALGKLRLEGQNTVATVMMSDIRGFTSLSEKEDPTIILKWLNEYY
                     QVVVPIITSFGGVVDKFEGDAMLSFFGILPKMLPAEDSAYLACKAGLELLRIIEELNT
                     KRASRGDPPLVTGVGINTGNLIAGGLGTADRLNYTIIGDAVNTTQRIEGVTRGFYESG
                     IVISETTLTALKDHRSEFYFEPLGEHALKGKSELIWLYRLWPENVGKHSQVRFESEVN
                     DEVQS"
     gene            complement(6053..6295)
                     /locus_tag="AC16CB_06440"
     CDS             complement(6053..6295)
                     /locus_tag="AC16CB_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342141.1"
                     /translation="MVGTGVFVGGGFVGIGVLVGGGFVGIGVFVGGGCVGIGVDVGGG
                     VVRVGVGAIDVGGFVVGSDVLVGDTAVNEVDSVAVC"
     gene            complement(6373..6603)
                     /locus_tag="AC16CB_06445"
     CDS             complement(6373..6603)
                     /locus_tag="AC16CB_06445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR3342142.1"
                     /translation="MPIYEYVCLDCGTRFDSLRPMKDADAPIGCEQCNSKHTSRLLSV
                     FFAQSSSGRIVAGGNGGACASCVSGSCASCGN"
     gene            6857..7669
                     /locus_tag="AC16CB_06450"
     CDS             6857..7669
                     /locus_tag="AC16CB_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342143.1"
                     /translation="MPASDGGFSQTRQVAYTAAAETVITQLTAVALTATPAVTPIPTE
                     AQVPTSIDLASETPLPATFTPTASPTSEVISMPTAVPDDPKLQLGEPDWQDKFKNGDN
                     WSLYEDEYVRFRVPDEVLIMTAFDAIGRDSWMLTWPEPENYYLEVVAIPETCEGLDRY
                     GIIFRTDADVGYLYGFSCDGQYSLRRWNGDTFKMLLEWTSSPYILPGADQMNRMGLMV
                     DDDQFSLYANGYFLEELSDDSYPDGGIGLFVASDETDDFKVQVSEVTYWELP"
     gene            7696..8256
                     /locus_tag="AC16CB_06455"
     CDS             7696..8256
                     /locus_tag="AC16CB_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342144.1"
                     /translation="MADNCTKRVRKLYDWEIEEFSAVFGDSLDYQRVRIHECTSWPDW
                     IDRLGRFLKRMPAPGLHDHNAVTLGNHCFFPVDMPEELLPVEHQMSFKHEWLVHEVTH
                     AWQFQRSGWIYLFKALISQFRDKEKAYDFEGESGLLKSREKGKLFKEFNPEQQGNITQ
                     SFYWRYRRKKPTQAWDPFIEEIRRVV"
     gene            complement(8293..>8817)
                     /locus_tag="AC16CB_06460"
     CDS             complement(8293..>8817)
                     /locus_tag="AC16CB_06460"
                     /inference="COORDINATES: protein motif:HMM:NF014411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HIR3342145.1"
                     /translation="LLNPGEYAVLTLHRASNVDDRETLYELLSIISKVSHRLPVVFPI
                     HPRTARRLEEAGLDSLIQSSPDLIICEPYGYLDFLSLVANARVVLTDSGGIQAETTIL
                     GIPCLTLRWNTEWPETIEQGTNHLVGTDQGRIFVALDEIFQARANQYSRPEGWDGKAA
                     GRIVKNILQHFHIR"
BASE COUNT         2290 a         1995 c         2215 g         2317 t
ORIGIN      
        1 atcggtgggg gatggacctc ccagcaggtt attgaggggc gtatacgttg ggagcctcgg
       61 gtaggtcgat tgacgcgcac ctaccaaaat atctctgcgc ctgcaattga agatgatcga
      121 gagattcgcc aaaatttagg cgctttcgat cgtaataaag ccgaagcata tcaggaagca
      181 gcgattaaag actcgtttat ccgtttgcca gaacgtgaaa cacaggacgc ctggacggat
      241 gctgttccag aaattcaagg tgttgctagc caagaatgcc tggaagcagc aggtgctgat
      301 catgtgagaa gttttaattg gacgcccaaa tatttgaacc ataattggtc tcttctcttg
      361 cagccaatct atacaaccta ttatctggac gatgaaaacc agccccagct cgtgtatgtt
      421 catgggcaaa gtgcaaaaat cagcggagtt cggcgggcgt ctatgaagcg tgcgcaacgg
      481 ctagctttaa tgatcttggg ggcagcaatt tttatggctt tccttagcct tgtaatcact
      541 gcgattggtg tagtggcgcc gcctttacta gttatcggcg gattaggtct gggagcttct
      601 gtatttgtag gattgggagc acttttacct ttagtgattg catggcagtt caaccgcaat
      661 cagaagggga tggcaaaata agcagatggc ttttgaagat aaacctctta tcacggcttt
      721 tgttagcgat ttatatttct catccaggat cgagtcggta acccaaagga tgggttttac
      781 cgtgttattt attgaacgag acgaggaaga tgagccaaac aatctgggcg atgttgatat
      841 ccaatatgct gaacatctgg atgggcaagg gggggcacta ttggataagc tcacccagca
      901 gcttccggca ttgatcattt ttgatctagg aaatgctgat atcacttggc gaaaatggat
      961 tctcttgatc aaatcgtctc cggccacgcg gcgaacacca gtgttgtgtt tcggatcaca
     1021 tatggatacg gagagcatga aagctgcaaa ggcagcaggc gcggacgcag tgctggctcg
     1081 ctctaaattt gtccagtcat tgagtgagct gatccaaaaa tatgcccgca gaattgatta
     1141 tggcgcgttg aaagatgctt gccagcactc gctttccgaa atcgccgtgc gtggtttgga
     1201 ggagttcaac cggggagaat attttgaggc acatgagtct ctggaggatg cctggaatca
     1261 ggatcaaact gcagggcgtg agctttatcg tgccatattg caagtcgcag tggcgtactt
     1321 acaaattgaa cgaagaaatt tccgtggtgc tatgaagatg ttcctgcgct tgcgccaatg
     1381 gattgaccct cttccagata tgtgccgggg ggtaaacgtg gcacaattac gaactgatgc
     1441 tcttgaggtg catgaagctc taaaggcttt agggccggag catatcaacg agtttgatca
     1501 cagcctattt gcccccgtgc tctatttcag gtgatcaact cgtgagccat cccgatccta
     1561 taattcccga aacctgggga caactcggtt tgggatctct aagaggggtc ttaatggttg
     1621 ttggagcacc agatacgggg aagagcacct ttgctcgtta tctgttcaaa cgcttgagcg
     1681 gagagggctt aagagtggca tatttggacg gcgaccccgg gcagagcacg ctcgggcctc
     1741 ctacaacact ttcgctggta attgcacatc ctggagaaag gcgatttccc ccagccggtc
     1801 agatttggcg ttggttcatt ggatctgtct cgcctcatgg gcacatgtta ccccttttgg
     1861 tggctgctgc gcgcctggtt cgagttgggt tggaaggtgg tgcggatgtg gtggtatacg
     1921 atacatgtgg gttgattgat ccaaatcagg gcggaatggc actgaagctg gcgaagatag
     1981 acttgctaca accccaggga ttgtttgcca tacaaattag tgatgagttg gaacccttac
     2041 taatcccaat gaggcgcagt aagcgtgccc gcgttgtgga tttatctagc tcctcggcag
     2101 tgatacctcg tgttcaagct gctcgacagg cataccgggc ggagcagttt gctggttact
     2161 tcagaaaggc aggtcgtgtt gatattagct ggagtagtca gcctgtgttc cctgcacccc
     2221 gcttcgctct caatcgttta gtatctttgg atgatgcaaa tggatttgca ctcggacttg
     2281 gtattgttct aaatgtaaat cgccaaatgc aatctgttga attgctcacg ccattaccat
     2341 cgttagaagg agtgcaatcc atcacgttag gcgatatcag cctggatcga gaaacatttc
     2401 gagactctat gatctgataa tagcctaggt ggttaaaaac aatcggagac cgaattacca
     2461 tggagtattt tgatttaatc caaaagcgtt acagcgtgcg agcctataag ccggatcctg
     2521 ttgaggatga caaactgcaa aaagtgctag aggcagcccg gttggcgccc acagcggcga
     2581 accgccagcc atttcagatt attgtgatcc acaccgaggg acggaaagac gagctaatga
     2641 agatttaccg gcgggattgg tttgtccagg cgccgttgct tatttgtgcg gtgggagtgc
     2701 cgcgcaccag ctgggtacgg gccgaccgtc gccgctacct tgatgtggat gtagccatcg
     2761 tcatggatca tttgatcctg gctgctacta gtttggggtt ggggacgtgc tgggtggcag
     2821 cttttaatga ggaaatggct cgaaaaagcc tgcacttgcc tgatgaggta gagcctcttg
     2881 tctttacccc attgggttac tcagctgacc aacctactga gaaagaacgt aaagagattt
     2941 cccagctcat acgatttgaa cattggtaaa gcagggttaa atcgccctag agatgagatc
     3001 tggctggtaa tcaccagcca gatcttgctt aacaaatttg tcagaattgt tatgctttgc
     3061 aacaatgtaa gtccatctgc ctagtattgg aaatcgcact gtcataaaat actaccaagt
     3121 ctcgtctcaa agatggttgg tgcgctaatc tcccctaacc ttggtttggg gaaaatattg
     3181 ggaatcgtca aaaactatga ataaacggat actgctgctg ctggcggatg taaaagcaga
     3241 gaaatctctc tcaaaatatt ttcaaacacg gggagatctg gtctggcata cgaacgatgt
     3301 atctaacgta ctggggcttg ttaaacgtta taagcctgag attgtggtta tcgatctgca
     3361 catgccagga gagaattggc tcgaggcttt gtcaactatc agaaaatatc gaccatcggc
     3421 taagatcatc atcaccaata aatatcccga ttttcgtcgt gagttaagag ccaaggaata
     3481 tggtgtcaaa gtatttctcc gtcagccatt taaacagaaa tggcttgata aggctatcca
     3541 ggctgttgag attggggagc aattgccagc gagcaaacgg acaatattac ctcgcgtacg
     3601 agttagcatg caggtaaaga ttacatttcc gtatgcattg ttggctttgt ttttcgcact
     3661 tgcttctgct ttccttatta gccgatatgt attggaatca ttacgtgatc gatttttaat
     3721 ccagcttatt gatactggga gtctaacagc tgattggatg gtccaggagg agaatcgcct
     3781 cttagagact ttacgcctaa tcgcaaactc tgagggggtt cctgatgcac ttttagcagg
     3841 tgatgtagag cgtttgcaga cgatcgcatt acctgtggca gttaattccc aggaagaagc
     3901 tatagagata ctttatgcca gcggtgaaat tatgctatcc ctccatcatc gcccgggagg
     3961 cacggtcgaa gactatactg ttgttcaagg agatcctcag ctcgcacagc aggtttttat
     4021 tagacaagtt ttgtatggac gagttgacga tcagggtgac aaatttgctg gattagcacg
     4081 ccttcaaaga ggtgattact tctacatcgc tggtcccatc tacgacacgg agggcaggct
     4141 ggttggtgcc gttgcggttg gggaatcctt gagcaaaatg gttcagtcga ttaggcggga
     4201 taccttagca cacgtcagca tctactccat ggatggcaat ccaatcattt caaccatttt
     4261 catggaagaa aacagtcgtc cgctccctgt tgaaattgta gccaaaatac tcaaatcgca
     4321 ggatagtgaa agtccggtgc gggagatgtc aatcagcaac accaattttt cagaaatcct
     4381 aggaccatgg gaggtgcgcg gaggccatga tcttgggatc attgggacaa ccctgagaca
     4441 aaattttata acacgcccat catttattac gcgctttcaa gtaattgcaa ttatcttggt
     4501 tggagtgtca ggggtcatct tgctcgggat ctatctcgca catcagatta ccagcccact
     4561 ttcaaaagta gtcaaagcat caatggaagt agctaaagga aattttgagg tcaaggtacc
     4621 ctcgcaaggc aacgatgaag tgatggtgct ggcgcacgcc ttcaattata tggtttctgg
     4681 cttgcaggaa ggttttattt accgagattt gctaggtcgc actgtttctc ctgaggtccg
     4741 ggaagctttg cgagagtcgt tcgcattggg gaaactacgt cttgaagggc agaacacggt
     4801 tgctacagtc atgatgagcg atatccgtgg attcacatcc ttgtccgaga aagaggatcc
     4861 cacaattatc ctgaaatggt tgaatgaata ctaccaagtt gtggtgccca ttattacctc
     4921 atttggcggt gtggtggaca aatttgaggg cgatgcaatg ttatcgtttt ttggaatatt
     4981 gcccaaaatg ttgccagctg aggatagcgc atacctagcc tgcaaagctg ggctggagtt
     5041 gcttcggatt attgaagagc taaatacaaa acgtgcatct cgaggagacc cacctctagt
     5101 taccggtgtt gggattaata caggcaattt gatcgcaggt ggattgggaa ctgcggatcg
     5161 gttgaattat acaattattg gtgatgcagt gaacacaacc cagcgaatcg aaggcgtcac
     5221 gcgcggtttt tatgaaagcg gcattgtgat tagcgaaacg acgctgacgg ctttaaaaga
     5281 tcatcgcagt gaattttatt ttgagccttt gggtgagcac gccttaaagg gcaagagtga
     5341 actcatctgg ttatatcgct tgtggccaga aaatgttggc aaacattctc aggttcgctt
     5401 tgaatcagaa gtcaatgatg aagttcagtc gtaagagttt atgggagcac ctgcgctggg
     5461 tctctctttt cttgatactg ttctttgctt tcctaatgat cgttttgatt ccactgcttg
     5521 tgagggttag ccagccagat tctgtgccat atgcccttca ctcggttttg agcgccaatt
     5581 atggcgctga tccacacaac ctgaagcatg ctcctcttcg gctgagtatc atcgcggatg
     5641 taatacgaga ccagcaggca atcgcctcgt ctaggttatc gcttaatgaa gttgggagcg
     5701 cgagcgactc ggaagtccca gcttcaagta ccgactctta tccagtaatt aactcgacac
     5761 cggggtccgg ttcagacaat aatcagaata caccagttgc tgcggatcta gcgtcgacac
     5821 agaggagcga tacttattcc actctccagg caagtgggac ggtcgactca acaggtatag
     5881 gttctacacc caccttaaag ttcacgcagg aacctgctca aacatcaata attaatatta
     5941 gaacaccaac tcctactccc aacaggacgg gtcaaataac accggtgtta actgggcaat
     6001 ctccaacagc aaaaccggct gtaacaaatg cgccgacaca aacgacttct gctcagcaaa
     6061 ctgcgacact atcgacttca ttgactgcgg tatcccccac taagacatcg ctgccaacca
     6121 caaaaccgcc gacatctatc gctccaaccc caacacgcac taccccgcct cctacatcga
     6181 cgccaatccc gacacaacca cccccaacaa ataccccgat ccccacgaag ccaccgccta
     6241 ccaatacacc aatacctacg aaaccaccgc ctacaaatac gccggttcct accaagccac
     6301 cggatcctta tcctcctcct ccacctccac cgccaccacc aacggatatt cccccgtatc
     6361 cataatcagg ggttaatttc cacagcttgc acatgacccg cttacacagc tggcgcaagc
     6421 accaccattg cctcccgcaa caatgcgtcc gctgctgctc tgcgcaaaga agacagagag
     6481 taagcgagaa gtatgcttac tattgcattg ttcacaacct attggtgcgt cagcatcttt
     6541 cattgggcga agagagtcga atcgtgtgcc gcaatcgagg caaacatatt cataaattgg
     6601 catatatcac ctttctacca ggtagattat ttttattcta actcttggat tccttcgggt
     6661 caatggacga aattctggtt ttccagatgt attttatcgg aactatctac ctacactagg
     6721 cgttataata ataagcctag tagcttcgat acttaacgga aggatgatgc tagatttcaa
     6781 ccctcgaatg aaaatccaac ttattctcag atcatgtatt agtctattgc ttcccttatt
     6841 gctcatttcg tgtagcatgc ctgcaagcga tggtggtttc tcacaaacca ggcaggttgc
     6901 ttacaccgct gcagcggaga ccgtaattac tcagctgact gcggtagctc tgaccgcgac
     6961 accagcggtg accccaatac ctaccgaagc gcaagtacca accagtattg acttggcaag
     7021 tgaaactcct ttgcccgcca cttttactcc taccgcttcg ccaacgagcg aagtgatttc
     7081 aatgccaacc gcggttccgg atgatcctaa gctccaatta ggcgagcctg attggcagga
     7141 taagtttaag aacggggata attggtcact ctatgaggac gagtatgtgc gatttcgtgt
     7201 tccggatgaa gtgctcatta tgacagcttt tgatgccata gggcgcgatt cgtggatgct
     7261 tacttggcca gagccggaaa attattatct ggaggtggtt gccatacctg agacttgcga
     7321 agggttggac aggtatggga ttatctttcg cacggatgct gatgtgggtt acttgtatgg
     7381 cttttcctgc gatgggcaat attcattgcg acgctggaat ggggatacct ttaaaatgct
     7441 tctggaatgg acttcaagcc cttatatttt acctggtgct gaccagatga accgcatggg
     7501 gttgatggtg gacgatgacc aattctcact gtatgccaat ggatatttct tggaagagct
     7561 cagtgacgat tcgtatcctg acgggggtat cggattgttt gtcgcttcag atgagacgga
     7621 tgatttcaaa gtccaggtga gtgaggtcac atactgggaa ttgccataac aaattaagct
     7681 caggaggtat tagcgatggc agataattgc accaagcgag ttcggaaatt atatgattgg
     7741 gagattgagg aattcagcgc ggtttttggt gattccctgg attatcagcg cgtgcgtata
     7801 cacgagtgta catcatggcc ggattggatc gaccgattgg gtaggtttct gaagcgaatg
     7861 ccagccccag gattgcacga tcataatgca gttaccttgg gaaatcattg ctttttccca
     7921 gtagatatgc ctgaagagct ccttcctgtc gagcatcaaa tgagcttcaa acatgagtgg
     7981 ctggtgcatg aggtcaccca tgcctggcag ttccaacgct ctggctggat atatctgttt
     8041 aaagcgctca tttctcagtt ccgcgataaa gaaaaagcct atgattttga aggcgaatcg
     8101 ggattgttga agagtagaga aaaggggaaa ttgttcaaag agtttaaccc agagcaacag
     8161 gggaacatca cccaatcatt ctactggcgc tatcgacgga aaaagcctac ccaggcttgg
     8221 gacccgttta ttgaagaaat taggcgagtg gtctaaagta ggagcaattg ggggtatttc
     8281 gttttcgaaa aactatctta tgtggaaatg ttgaagaatg ttcttcacaa tacgacccgc
     8341 tgccttacca tcccagccct ctgggcgaga atattggtta gctctagctt gaaatatttc
     8401 atcaagagca acaaagatac gcccttggtc ggtaccgact aagtggtttg tgccctgctc
     8461 gattgtctcg ggccattcag tattccagcg caacgtcagg caaggtatcc ccagtatcgt
     8521 tgtttcagct tgaatccctc ccgagtctgt taaaactact ctagcattgg cgaccaggga
     8581 caagaaatcc aaatacccat atggttcaca aatgatcaag tccggagaag attggattaa
     8641 ggaatcgaga cctgcctcct ccaggcggcg tgctgtacgc gggtgaattg gaaaaacaac
     8701 tgggagacga tgggatacct ttgaaattat tgacaataac tcatacagtg tttctcgatc
     8761 atccacattg gaagcgcggt gcagggttag aacagcatac tcgcccggat ttagcaa
//
LOCUS       DBNGSX010000211         3249 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_155019, whole genome shotgun sequence.
ACCESSION   DBNGSX010000211 DBNGSX010000000
VERSION     DBNGSX010000211.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_155019"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..82)
                     /locus_tag="AC16CB_06465"
     CDS             complement(<1..82)
                     /locus_tag="AC16CB_06465"
                     /inference="COORDINATES: protein motif:HMM:NF014757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HIR3342146.1"
                     /translation="MEIQRIFVIGAGTMGNGIAQVAATSGY"
     gene            complement(177..1385)
                     /locus_tag="AC16CB_06470"
     CDS             complement(177..1385)
                     /locus_tag="AC16CB_06470"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIR3342147.1"
                     /translation="MDNHDRQPVILSAVRTPSGRFQGSLSGFPAPKLGALAIKVAVER
                     AKINDPAQIEEVFMGNVVTAGEGQAPARQAAIFAGLPASVGATTINKVCGSGLKAVML
                     AAQAIKAGDGDLFVSGGMESMSRAPYLVDGRTGALRYGHVQLTDALLHDGLWDPFEDW
                     GMGNAAEFIAEEYEVTRQAMDEFALRSHQRALAASDAGKFKAEIVAVEIPGKKGQVTV
                     FDTDETPRRDTSLDVLASLKPAFQKDGKVTAGNAPGLNDGAAAVVVASRSKADAMSTK
                     PLARIVGQAQAAVEPKYLFIAPAKAIPRLLDKVGWTLADVDLIELNEAFAAQVLADGY
                     ALADMGWDWDKVNVNGGGIALGHPIGASGARVLTTLIYALKDRGLKRGIASLCLGGAE
                     AVAMAIEIED"
     gene            complement(1434..1931)
                     /locus_tag="AC16CB_06475"
     CDS             complement(1434..1931)
                     /locus_tag="AC16CB_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012571398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR3342148.1"
                     /translation="MKHLWSPWRMSYIQNYNRKNECAFCIELSRPDGPENLIIYRGQR
                     AFIILNRFPYNSGHLMVVSNEHKSTLDELDAETRAEIMELSNLATRALQAVYNPHGFN
                     LGLNIGDAAGAGILEHVHMHILPRWKGDTNFLSALDETRVIPEALEETYKRLKKGLED
                     ILRSR"
     gene            2090..>3249
                     /locus_tag="AC16CB_06480"
     CDS             2090..>3249
                     /locus_tag="AC16CB_06480"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013713.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermine/spermidine synthase domain-containing
                     protein"
                     /protein_id="HIR3342149.1"
                     /translation="MRRISITNSAARLFLLSFLVLFFELVCIRWLSSYVLYLGYFTNF
                     VLLGCLLGIGAGVLLANYQVRLINYLPVLLFSFFPLILFIQPQVTPSSQNFIYFTNNL
                     AWLQLPAYILLPLVFISITAIFTMLSQELGFLLNEFAPLRAYNINILGSLAGILCFMV
                     LGYFSLPSWTWFFISVTILVILLPRDRFFKRNVLLQIGLVIVIASSDYAFANLWSPYY
                     RVNMLELSNEGYQRVRYPIVNKEIEKRVLFANGVAHQEFALVSKNLPFYNLPYDAFAE
                     HQQYEKILIVGAGGGNDVSFALKNQVKQVDAVEIDPLIAKLGGTYHPENPYRDPRVTL
                     YVDDARSFLEKSDTYYDLIIYALPDSLILAANTSNIRLESYLFTLEAFQSIK"
BASE COUNT          704 a          841 c          785 g          919 t
ORIGIN      
        1 ggtagcccga cgttgccgcc acctgggcga tgccattgcc cattgtccct gccccgatga
       61 caaagatacg ctgaatttcc atagactagc tccttagatg aaaaggggag aaatcgatgg
      121 ggggtatcta tcgtgctctg ctgctttctc ttacccctat cccctctccc caatatttaa
      181 tcttcgatct ctattgccat tgccaccgcc tccgcccctc ccaggcacaa ggaggcaatc
      241 ccgcgcttca gaccgcggtc tttcagcgcg tagatcaggg tggtcagcac gcgtgccccg
      301 ctagctccaa tagggtgtcc caaggcgata cccccgccgt tcacgttcac cttgtcccag
      361 tcccagccca tgtctgccaa agcatagcca tcagccagca cctgcgcggc gaaggcctcg
      421 tttagttcga tcaggtcaac gtcggctagc gtccatccca ccttatcaag cagacgaggg
      481 atggcttttg caggggcgat gaaaagatac ttgggctcaa cagccgcttg agcttgcccc
      541 acaatgcgcg ccagcggctt tgtactcatt gcatctgctt tggagcggct tgccaccacc
      601 acagccgctg ccccgtcgtt cagcccgggg gcgtttccag cggtgacttt gccgtctttt
      661 tgaaaagctg gcttcaagct ggcgaggaca tctagcgatg tgtcccgccg gggcgtctca
      721 tcggtatcga agaccgttac ctggcctttc ttgccgggta tctcgacggc tacaatctca
      781 gctttaaact ttccagcgtc gctggcagcg agtgctcttt ggtggcttcg cagggcgaac
      841 tcgtccattg cctggcgggt gacttcgtat tcctcggcga taaactcggc tgcattgccc
      901 atcccccaat cctcaaatgg atcccacaag ccgtcgtgta acagggcatc ggtgagctgg
      961 acatgcccgt aacgcagtgc gccggttcgc ccatccacca gatagggtgc ccggctcatg
     1021 ctctccatcc cgccggacac aaacaggtcg ccgtcgccgg ctttaattgc ttgtgctgcc
     1081 agcatcacag ctttcaggcc tgaaccacac actttgttga ttgtggttgc ccctacactg
     1141 gctggcaagc cggcaaaaat cgctgcctgg cgggctggcg cttgcccttc cccagccgtg
     1201 accacatttc ccatgaagac ctcttcgatt tgggcggggt cattgatctt agcgcgctct
     1261 actgctactt tgattgctaa agctcccagc ttgggagcag ggaagccact caggcttcct
     1321 tggaaacgac ccgaaggcgt gcgtactgcg ctcaggatga cgggttggcg atcgtgattg
     1381 tccatgtggt agccctccgt gttgcggatt atgtcgttat ggatccgtgt ttgttaccta
     1441 ctgcgcaata tatcctctaa cccctttttc aaccgcttat atgtctcttc caaagcctct
     1501 gggatgacac gcgtttcatc caatgctgac aagaagttcg tatccccttt ccagcgcggc
     1561 aggatgtgca tatggacgtg ttcgagtatg cctgctccgg ccgcgtcgcc gatattcaac
     1621 cccaggttga agccgtgagg gttgtatacg gcttgcagcg ccctggtcgc cagattggac
     1681 agctccatga tctcggcacg cgtttcggca tccagctcgt ccaacgtgga tttatgctcg
     1741 ttagagacca ccatcaggtg gccgctgttg tatgggaaac ggtttaaaat gataaaagct
     1801 cgctgaccgc ggtagataat taagttctcc gggccgtcgg gccgtgacaa ttcaatgcag
     1861 aaggcgcact cattttttcg gttgtagttt tgtatgtacg acattcgcca gggagaccag
     1921 agatgtttca taaaggtagc ttttcacctg cggtgatagt tcaaggctat tgtagcaaat
     1981 taagcgaatt tcaggaagtt tgatatcccg gatcgataga tcgcttgtga aggtattacc
     2041 ttaatcgtat aatggcaagc atattttaga ttccatgagg aagggcagga tgcgtagaat
     2101 ctccataaca aacagcgccg cacgtttgtt tctgctgagc tttttagtat tattctttga
     2161 actcgtctgt atccgttggt tatcctccta tgttctttac ctggggtatt tcacgaactt
     2221 tgtactgcta ggttgtttgc tagggattgg tgcaggtgta ttgttagcta attaccaggt
     2281 gaggctgatt aattacttgc cagttttgtt attttcgttt ttccccttga tcttattcat
     2341 tcaaccccaa gttacaccaa gcagccaaaa ctttatctac tttacaaaca atttagcttg
     2401 gctacaatta ccagcttata tacttttgcc tctcgttttt atcagtataa ctgccatatt
     2461 tactatgctc tctcaagagc taggtttctt gttgaatgaa tttgctccac tcagggctta
     2521 taacataaat atcctgggga gtttggcggg aattttgtgc ttcatggtat taggttattt
     2581 ttcgcttcct tcttggactt ggttttttat atcagttacc attcttgtga tattgttgcc
     2641 gcgtgatcgt tttttcaaaa gaaacgtgct cttgcagatt ggtctggtga tcgtaatcgc
     2701 ttcaagtgat tatgcctttg ccaatttatg gtcgccctat tatcgggtga acatgctgga
     2761 attaagcaac gaagggtatc agcgagtaag atatcccatc gttaataagg aaattgaaaa
     2821 acgtgttctg ttcgctaatg gtgtagccca tcaagaattt gcactagtca gcaaaaatct
     2881 ccccttctat aaccttccat acgatgcttt tgctgagcac cagcagtacg aaaagatttt
     2941 gattgttggg gccgggggag ggaatgatgt ttcattcgcc ttgaagaatc aggtgaaaca
     3001 agtagatgcc gtggagatcg atcctctcat tgcgaaatta ggtggaactt atcatccgga
     3061 aaatccttat cgtgacccga gggtcacgct ttatgttgat gacgcacgca gcttcctaga
     3121 aaaatcggac acttattacg atttgatcat atatgctttg cccgactcac taatacttgc
     3181 tgcaaatact agcaatattc ggcttgagag ctacttgttc acgcttgaag cttttcagag
     3241 catcaaaga
//
LOCUS       DBNGSX010000212         1604 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1609401, whole genome shotgun sequence.
ACCESSION   DBNGSX010000212 DBNGSX010000000
VERSION     DBNGSX010000212.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1609401"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1477
                     /locus_tag="AC16CB_06485"
     CDS             <1..1477
                     /locus_tag="AC16CB_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3342150.1"
                     /translation="HPSTVEFINSGPKKLLISGEWVDAASGETFATINPATGEELTQV
                     ALAGSQDVDRAVKAARQAYEHGPWSKFSGEERGHLLWKVADLIEAHADELAELDTLDN
                     GKPLRVARRGDLPSAARHFRYYAGLAGKIEGSTIPVSMPNQFVYTRREPMGVVGLIIP
                     WNFPLLMCAWKLAPALACGNTALLKPAEETPLSALRLGDLIEEAGIPPGVVNILTGPG
                     LPTGAAIVAHNDVDKVAFTGSTQVGREIMKNAAASNLKRISLELGGKSPNVIFADADL
                     GKAIRGANWAIFSTSGQECVAGSRLFVERPVYEQVIEGLAAEAQKIRVGNGFTPKVHI
                     GPIISEQQLQRVLGYIASGRSGGAQVVTGGQRLGGELESGYFLSPTIFTHQDDTLSIV
                     QEEIFGPVVAATPFDEWDDLVQRANMTHYGLAAGVWTRDISKAHRFAHAMKAGTVWIN
                     GYGHFDAAAPFGGYKESGFGREMGTDAIELYTQVKTVWVGL"
BASE COUNT          360 a          425 c          474 g          345 t
ORIGIN      
        1 acacccttca accgtagaat ttatcaactc tggccctaag aaactcttga ttagcggtga
       61 atgggtggat gccgccagcg gggagacctt tgcgacgatc aaccctgcta ccggggagga
      121 gctaacccaa gtggcactag ccggctcgca agacgtggat cgcgccgtga aggctgcccg
      181 ccaagcttat gagcacggtc catggagcaa atttagcgga gaggagcgcg gtcacctgct
      241 ctggaaagtg gcagacctga tcgaggcaca cgccgacgaa ctggcagagc tggatacgtt
      301 agacaatggc aaacccctgc gagtggcgcg gaggggagac ctcccctccg ctgcccgcca
      361 ttttcgctat tatgctggtt tggctggcaa gatcgaaggc agcacaatcc cagtaagcat
      421 gccaaaccag ttcgtatata cccgccgcga gccgatgggt gttgtggggc tgattattcc
      481 atggaacttc ccgttgctca tgtgcgcctg gaagcttgcc cccgcactcg cttgtggcaa
      541 caccgccctg ctcaagccag ccgaagaaac tccgctgtct gccttgcggc tgggcgatct
      601 aattgaagaa gctggcatcc ctccaggagt ggtcaatata ctgactggac ccggcttgcc
      661 caccggggct gcgattgtgg cccacaatga cgtcgataaa gtagctttca ccggctccac
      721 tcaagtgggt cgcgagatca tgaagaatgc cgctgcaagc aacttgaagc ggatcagcct
      781 tgagctgggt ggaaaatcgc caaacgtgat ttttgccgac gccgacctgg gcaaggctat
      841 ccgtggcgca aactgggcga ttttcagcac gagcgggcag gaatgtgtgg ctggttcgcg
      901 actcttcgtc gagcgcccgg tgtacgagca ggtaatagaa ggattggcag ccgaggcaca
      961 gaagattcgt gtgggcaatg gatttactcc caaagtacat atcggcccaa taatttccga
     1021 gcagcaactc cagcgagtct tggggtatat cgccagcggg cgcagcggtg gagcccaggt
     1081 agttacaggt gggcaacgcc tgggtggaga attggaaagc gggtatttcc tttcccctac
     1141 catttttacc catcaggacg acaccctatc catcgtacag gaagagatct ttggtccagt
     1201 ggtagctgcc acacccttcg atgaatggga tgatctggtg cagcgcgcca acatgaccca
     1261 ctacgggctg gctgccggag tttggacgcg ggatatcagc aaagcccatc gatttgctca
     1321 cgccatgaag gctggcacgg tatggattaa cggctatggt catttcgacg ccgctgcgcc
     1381 ctttggaggc tacaaagaga gtggttttgg ccgcgagatg ggcacagatg cgattgagtt
     1441 gtatacgcag gttaaaacag tttgggttgg tttatagata ggtatataga tgaacgccag
     1501 cctaccagat cgcctgacac tagccgcttt ttcagtagtg gtgcttcttg gaggcttgaa
     1561 cgcggttgcg gttcgtttca gcaatcagga acttccccca ttct
//
LOCUS       DBNGSX010000213         2768 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1263589, whole genome shotgun sequence.
ACCESSION   DBNGSX010000213 DBNGSX010000000
VERSION     DBNGSX010000213.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1263589"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="AC16CB_06490"
     CDS             <1..333
                     /locus_tag="AC16CB_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3342151.1"
                     /translation="LTAHYRSIVTWERFTIDVSVLYLAMIIIGGLGSVPGSFFGATFM
                     TLLPAVLTNLGRALKSTFPIIETVIPFIQLAAFGLVIILFLIFEPEGLNKMWRNIKDY
                     FRLWPFSY"
     gene            431..1642
                     /locus_tag="AC16CB_06495"
     CDS             431..1642
                     /locus_tag="AC16CB_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3342152.1"
                     /translation="MFQRNRTIQTWMVVFILATLLLSACGGGGGAGETSPVKVGAIFD
                     LTGATADVGTPYSEGLKAYVEWRNANGGINGHPIELISADYAYKVDVAEQLYTQYVQE
                     GAVAFMGWGTGDTEALRSKIASDKIPFMSASYSANLLDMAQAPYNFLIGTSYSDQALI
                     ALRWAAEDWEASGGAGPAKVAFIHHDSPFGKSPIPDAEAYAQAHGIEFVSLPMPGGVT
                     DYVAELAQVQQFGAGYIVVHTVSSPAAVLVKDIVSQGMKDKVKVINLNWCSDEIFINL
                     AGDAAEGVIGAIPFTPPSSPVPGHDDADKWLKDKGSSLAEKGLHFTQGWWTMAVMSEG
                     IKKVLDEGKEVNGENIKAALEGIQNYDTKGITAPISFSSSSHKGNSALRLFVVEGGQW
                     KQRSDFISAVP"
     gene            1715..2506
                     /locus_tag="AC16CB_06500"
     CDS             1715..2506
                     /locus_tag="AC16CB_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3342153.1"
                     /translation="MLTLNNVEVIYNEVILVLKGMSMDVPQGKIVGLLGSNGAGKSTT
                     LKAISGLLKAEDGEVTDGSIEFMGQPVHHRDAADIVRMGIFQIMEGRRVFEHLTAEEN
                     LVAGSYTRQDGKVRDDIDMVYQYFPRLKERRNQTAGYLSGGEQQMLAIGRALMARPKL
                     MMLDEPSLGLAPMLVQEIFGIIKRINSEHGTTILLVEQNANLTLGVADYAYIMENGRI
                     VLDGYPADLRENADVREFYLGLTEVGQRKSYRDVKHYKRRKRWLS"
     gene            2509..>2768
                     /locus_tag="AC16CB_06505"
     CDS             2509..>2768
                     /locus_tag="AC16CB_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HIR3342154.1"
                     /translation="MDLPDLVAHLAENAPGFAARIKAAGLTLDDLGNPNALDALPVVR
                     KDDLVESQAANPPFGGFLACAPGELKRIFQSPGPIYDPESRVA"
BASE COUNT          678 a          636 c          766 g          688 t
ORIGIN      
        1 ctcacagccc attatcgtag cattgtgacc tgggagcgat ttacaattga tgtgtcggtg
       61 ctttatctgg ctatgatcat tattggtgga ttgggctcag taccaggctc gttctttgga
      121 gcaaccttca tgacccttct acccgcggtg ctgaccaacc tggggcgcgc ccttaaatct
      181 acgttcccca ttattgagac ggtgatccca ttcattcaac tggccgcatt tgggttggtg
      241 atcatcctgt ttttaatttt tgaaccagag gggttgaata aaatgtggcg taatataaag
      301 gattattttc gtctgtggcc gttcagctat tagctcaatg aagaacagag gaggtgctct
      361 ttgcaggatg ccaaaatatt cgtttcaata ttagccaatc cataatccat caatcagagg
      421 aggagacatt atgttccaaa gaaatagaac tattcaaacc tggatggttg ttttcatcct
      481 ggcaacactg ctgctctcgg catgtggtgg cggaggaggc gcaggagaaa cgagcccggt
      541 caaggtcggc gcaatatttg atctcaccgg cgcgacggca gatgtaggta ccccatattc
      601 cgaagggctt aaagcctatg tggaatggcg caatgccaac gggggtatca atgggcatcc
      661 gatcgagttg atctcggctg actatgcata caaggtcgat gttgccgagc agttatatac
      721 ccaatatgtc caggaagggg ctgttgcctt catgggttgg ggtactggcg ataccgaagc
      781 tctgcgatca aaaatcgcat cagataagat ccccttcatg tcggcttctt actctgccaa
      841 cttgttggat atggctcaag ccccctataa tttcttgatt ggcacatctt actccgatca
      901 agcgctcatc gcgttgcgct gggctgcaga ggactgggaa gccagcggtg gtgcaggacc
      961 ggctaaggta gcctttatcc atcacgacag cccatttggg aaatctccaa ttcccgatgc
     1021 agaggcttat gctcaagccc atggtatcga gtttgtgagc ctgcccatgc ccggcggtgt
     1081 gacggactat gtggctgagc tagctcaagt acagcaattt ggagctggtt atattgttgt
     1141 ccacacagta tccagcccgg ctgctgtgct ggttaaggat attgtcagcc agggcatgaa
     1201 ggataaagtc aaggtcatca acctgaactg gtgttcagat gaaattttca tcaacctggc
     1261 tggggatgca gctgagggtg ttatcggagc gatccccttc accccgccta gctctcccgt
     1321 cccagggcac gatgatgcag acaaatggtt gaaagataag ggctccagcc ttgcggagaa
     1381 gggcctgcac tttacacaag gttggtggac gatggcggtt atgtccgagg gtatcaagaa
     1441 agtcttggat gagggcaaag aagtcaacgg cgaaaacatc aaagccgccc tggaaggtat
     1501 ccaaaactac gacaccaagg ggattactgc cccgatttcc ttctcctcca gcagtcacaa
     1561 gggtaacagc gcccttcggc tgtttgtggt tgaaggtggt caatggaagc agagatctga
     1621 ttttatctca gcggtccctt aagagatcgc ccgtggagga tggcagagga gtgtcaggtg
     1681 agagcccctg ccatcctcca aaaatggttg aaatatgctc acgcttaata acgtagaagt
     1741 aatttataac gaagtcatcc tggttttaaa aggcatgtcg atggatgtgc cgcaagggaa
     1801 aattgttggg ctgcttggtt cgaatggcgc gggaaagtcc accaccttga aggcaatctc
     1861 aggcttgtta aaggcagagg atggagaggt cacggatggc tcgatcgagt tcatgggcca
     1921 acctgttcat catcgtgatg cagcggatat cgttcgaatg ggcattttcc agattatgga
     1981 ggggagacgg gtattcgagc atctcacggc ggaagaaaat ctggtcgcag gaagctacac
     2041 ccgccaggat ggcaaggtga gagacgatat agacatggtt taccaatatt tcccgcgctt
     2101 aaaagaacgc aggaatcaga ccgcgggcta cctctctggc ggtgaacagc agatgctggc
     2161 tatcggccgt gcgttaatgg ctcgaccaaa gctgatgatg ttggatgagc catccttggg
     2221 gcttgctccg atgctagtcc aggagatttt tgggattatc aagcgtatta atagtgagca
     2281 tgggacgaca attctgttgg tcgagcaaaa cgccaacctg actttaggtg tagcagatta
     2341 cgcctacatt atggagaatg ggcgtatcgt cctggatggt tatccagccg acttgcgcga
     2401 gaatgctgat gtgcgcgaat tttatctcgg cttaacggag gtcggtcaaa gaaaatccta
     2461 tagagacgta aagcactaca agcgtcgcaa gcgctggcta tcgtaagaat ggatctacca
     2521 gacctggttg cccacttagc agagaatgcg cctggttttg ccgcgcgtat caaggcggca
     2581 ggcttgacac tggacgatct gggcaatcca aacgcattgg atgcgttgcc cgtcgttcgc
     2641 aaggacgatt tggtggagtc ccaagcagct aatccaccct ttggtggatt tttagcttgt
     2701 gcacctggtg agttgaaacg catctttcaa tccccaggtc caatctatga tcctgagtcg
     2761 cgtgtagc
//
LOCUS       DBNGSX010000214         2792 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1679226, whole genome shotgun sequence.
ACCESSION   DBNGSX010000214 DBNGSX010000000
VERSION     DBNGSX010000214.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1679226"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="AC16CB_06510"
     CDS             <1..368
                     /locus_tag="AC16CB_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR3342155.1"
                     /translation="LPMRDRMVGYILEEIPNSQLTGHPTKRLPNHASFVFEGVDGNAL
                     LMMLDVAGYACSSGSACKTGDPEPSDVLIALGLPRSWSLGSLRVTLGKDTTPLEVESF
                     LSDLPRIVEQARSLSEYVK"
     gene            358..1440
                     /gene="mnmA"
                     /locus_tag="AC16CB_06515"
     CDS             358..1440
                     /gene="mnmA"
                     /locus_tag="AC16CB_06515"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR3342156.1"
                     /translation="MSNKGRVVVAMSGGVDSSVAAAVLKQQGYEVIGLMLRLWSEPGA
                     EQTNRCCTPDSMAQARRVAAILDIPFYAVDAQETFYSQVVQSFIDGYTQGFTPNPCLV
                     CNRQIRWTFLLDRALALDAQYLATGHYARINRDDQDLYQLRKAIDHSKDQSYVLHVLD
                     QERLSHALFPLGDYTKSRVRELARHYDLPVAERADSQDLCFLGDGDYRKFLIRNAPRV
                     SHFGPITTKDGQLLGEHQGLAFYTIGQRKGLGLQSSEPMYVIEKDLQRNTLVVGSRQD
                     LGNSELIASNVNWISGKKPLAPFRAQVKIRYKASEAWANVFPLDDRQVQVLFDEQVRD
                     ITPGQAAVIYNGEVCLGGGIIQSSTG"
     gene            1446..1715
                     /locus_tag="AC16CB_06520"
     CDS             1446..1715
                     /locus_tag="AC16CB_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342157.1"
                     /translation="MTLPVLLFGILLSTLYGAAYHLWRGGGAGKLLLFLILGWIGFWA
                     GHALGNYLDWTFGSLGQLRLGMATLSSLIFLSIGHWLSLVEVERK"
     gene            1736..>2792
                     /locus_tag="AC16CB_06525"
     CDS             1736..>2792
                     /locus_tag="AC16CB_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TolB-like translocation protein"
                     /protein_id="HIR3342158.1"
                     /translation="MFRIESFLSARLFVRPQIIGNRIYFESNISGHISLYAMNFGGSV
                     PEPLLPPDVVLHNPHLMNGKSFYVYPKLGKIVVMIDQDGDENYQPMIIPIEGGYPKPT
                     FNDFFSEYRVYIDKCDPERNLLYLTADSRREELVFTYQGNLETGELTLLGQSQWGLYP
                     VGVNTDHTKVVILEEYTFGDEVLYLWTQKDGLGKPLHGVPIETRADGQKVPLSYITST
                     CFTPDDRGLIFVTALHEDTRGLGYLTFEHPEDVLRVDITGIMHQGIGELESLEEIGGD
                     RYLLTFNINGSSWLYEGNFDELNLTMKLNHVLCGTGQISNGVLESVDYDRHQDRFALA
                     FSTATSPTQIYTIEGKDR"
BASE COUNT          721 a          662 c          665 g          744 t
ORIGIN      
        1 tgcttccaat gcgtgatcga atggtcggtt acattttgga agagattccc aactcgcagc
       61 ttaccgggca tcccaccaaa cgattaccca atcatgcaag ttttgttttc gaaggggtag
      121 atggcaatgc gttattaatg atgctcgatg tagcagggta tgcatgctca tccggttcag
      181 cctgtaaaac cggagatcca gagccttcgg atgtgctcat tgccctaggt ctgcctcgtt
      241 catggtcgct gggttccctg cgtgtgaccc tggggaaaga cactacccct ctggaggtgg
      301 aatcatttct ttcagacctc cccagaatag tagaacaggc acgtagtttg agcgaatatg
      361 tcaaataaag ggcgcgtggt agttgctatg agcggtggag tcgatagctc tgtagccgcc
      421 gctgtattga aacaacaagg ttacgaagtc atcggcctga tgctgcgcct gtggagtgag
      481 cccggagccg aacaaactaa tcgttgttgt acacctgact cgatggcaca agcgcggcgg
      541 gtagcagcca ttctcgatat ccctttctac gcggtagatg cacaagaaac cttctattca
      601 caggtagttc aaagctttat tgatggctat acgcaaggct tcacgccgaa tccttgtctg
      661 gtttgcaatc gtcaaattcg ctggactttt ctgctcgatc gtgcgcttgc tttggacgca
      721 caataccttg ctaccggtca ctatgcccgc ataaatcgcg atgaccaaga cctctaccag
      781 ttgcgaaaag ccatcgacca ttcaaaagac caatcctacg tcctgcacgt acttgatcaa
      841 gaacgtctaa gtcacgcact ttttcctctc ggtgactata cgaaatcacg ggtgcgagaa
      901 ttggctcgcc attatgatct ccctgtcgct gagagggctg acagtcagga tttatgcttc
      961 cttggtgatg gagattaccg aaaattcctt attcggaatg cccctcgagt cagccatttt
     1021 ggaccaatca ccactaaaga tgggcagcta ttaggtgaac atcaaggttt ggctttctac
     1081 accattgggc agcgcaaagg tttggggttg caatcttctg agcccatgta tgtcattgaa
     1141 aaagatctac aacgcaacac actcgtagtc gggagcaggc aagatctggg caatagcgaa
     1201 ctaattgcct caaacgtgaa ttggatatct gggaagaagc cacttgcacc attccgtgcc
     1261 caggtaaaga tccgctataa ggccagcgaa gcctgggcaa atgttttccc ccttgacgat
     1321 agacaggtac aagtattatt tgacgaacaa gtgcgtgata ttacccccgg acaggctgcg
     1381 gtaatttata atggcgaggt ttgtttaggc ggcggaatta ttcaatcatc cacaggataa
     1441 aacacatgac cctacctgtc ttgctattcg gaattttgct atcgacactc tatggcgcgg
     1501 cttatcatct ctggcgaggc ggaggagctg gcaaactatt gctcttcctc atcctgggat
     1561 ggattggctt ctgggctggg cacgcccttg gcaattatct tgattggaca ttcggaagct
     1621 tgggacagct cagactggga atggctacac tgtccagctt aatttttctc agtattggcc
     1681 attggctgag cctggtagaa gtcgaaagaa aataaggagt aaccaaagag agttcatgtt
     1741 tcgaattgaa tcatttcttt ccgcccggct atttgtcagg ccccaaataa tcggtaatcg
     1801 aatatatttc gaaagcaata tcagcggaca tatcagccta tacgctatga atttcggtgg
     1861 cagtgtacct gagcctcttt tgcctccgga tgttgtcctt cataatcccc accttatgaa
     1921 cggaaaatct ttttatgtgt atccaaagtt ggggaaaatc gttgtcatga tcgaccaaga
     1981 cggtgatgaa aattaccagc ccatgatcat tccgatcgaa gggggctatc ccaagcctac
     2041 attcaacgat ttcttttcag aatatcgtgt gtacattgac aagtgcgatc cagaacgcaa
     2101 tttgctctat ttaaccgctg attcacgtcg tgaggagctt gtcttcacat accagggcaa
     2161 tctggagact ggtgaactta ccctgttagg ccagagtcag tggggactgt atccagtggg
     2221 cgtcaacact gatcacacaa aagttgtcat actagaagag tataccttcg gtgatgaggt
     2281 actctattta tggacacaaa aagatggtct tggtaagcct ttacatggcg ttcccatcga
     2341 aactcgagcc gatggacaga aagttccatt aagttacatt acctccacat gctttacccc
     2401 tgatgatcgc ggtctcatct ttgttaccgc gctgcatgaa gatacacgcg gactggggta
     2461 tttgaccttt gagcatcctg aagacgttct acgagtggat atcacaggga ttatgcatca
     2521 aggaatcggc gaacttgaaa gccttgaaga aattggaggt gatcgctact tgttgacctt
     2581 caatattaac ggttcttcat ggctatacga aggtaatttc gatgaactta acctaaccat
     2641 gaagctaaat cacgttcttt gcggtacagg gcaaatctcc aatggagttc ttgagtccgt
     2701 tgattatgat cgccaccagg atcgattcgc cctagcattc tctacagcta ccagtccaac
     2761 tcaaatctac accatcgagg gcaaagatcg ca
//
LOCUS       DBNGSX010000215         9428 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_155282, whole genome shotgun sequence.
ACCESSION   DBNGSX010000215 DBNGSX010000000
VERSION     DBNGSX010000215.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9428)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9428)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9428
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_155282"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="AC16CB_06530"
     CDS             <1..566
                     /locus_tag="AC16CB_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR3342159.1"
                     /translation="IANPADEHIAALPGILERFPPNQVVWAGPTHASSSARYLQSTLS
                     EVGMQPIPAQSGQTLDLGQGATLQILSANARGAVLLLEWRDFSALLPMGLDTESLQSL
                     PDRFAPVTALLLAEGGYAPLNPPEWIANLHPQVVLLSVSPGDHHGLPSPETLEAVQDY
                     TLLRTDRNGWIELTTDGEQMWVEVEKR"
     gene            complement(695..1915)
                     /locus_tag="AC16CB_06535"
     CDS             complement(695..1915)
                     /locus_tag="AC16CB_06535"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIR3342160.1"
                     /translation="MKLSKRIVLISVLVLSMLLALGPASGAKGLGHISERIYGTTIGG
                     QDVHEYTLTNAGKRPIEVKIITYGGIITSIKVPDKWGNMENVALGFDNLSDYETKNPY
                     FGCITGRYANRIDEGRFTLDGVTYCLDINNPPNSLHGGYVGFDKKVWEVTKAEVGPDG
                     VVLELHYLSLAGEGYEPAECADGEAGYPGNLDVYVTYTLNNKNQIVMDYVATTDAPTV
                     VNLTNHTYWNLAGEGEGDINDHILYLNADRFTPVDPTLIPTGELAPVAGTPFDFTVPK
                     PISDGLRSNAEQVAIGRGFDHNWVLSRPSFDDTSMLLAATLCEPGSRRTLNVWTEEPG
                     IQFYAGNFLDGTLYGTSGRAYRQGDGIALETQHFPDSPNQPGFPSTVLRAGETYATRT
                     IFELLVNKGQCRTK"
     gene            2922..3113
                     /locus_tag="AC16CB_06540"
     CDS             2922..3113
                     /locus_tag="AC16CB_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342161.1"
                     /translation="MKVLVLGGTGLCGKDGAPLLVQLRLGYATDLFIDMTKATMEFGV
                     RLTSHDEYIQKVLVNAPVH"
     gene            3232..3543
                     /locus_tag="AC16CB_06545"
     CDS             3232..3543
                     /locus_tag="AC16CB_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342162.1"
                     /translation="MLGAVRDGFVVTGLRRARGLLGVVSRYWFPYDPTLVSDDTTLVV
                     GWLGSVVLLSVSSGDRQSLPTLETLEVMQGHTLLRTDRNGWIDPTMDGAQLWVEVEKR
                     R"
     gene            complement(3565..3849)
                     /locus_tag="AC16CB_06550"
     CDS             complement(3565..3849)
                     /locus_tag="AC16CB_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342163.1"
                     /translation="MNQQSVAPKINWFVIVLILAASILLVTTIVTRATQWARLDELAG
                     FKNEVSPVDARLTGPSRLEELARIKDELLPVEARLSGPSRLEELVRIKER"
     gene            4182..8984
                     /locus_tag="AC16CB_06555"
     CDS             4182..8984
                     /locus_tag="AC16CB_06555"
                     /inference="COORDINATES: protein motif:HMM:NF044287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nSTAND1 domain-containing NTPase"
                     /protein_id="HIR3342164.1"
                     /translation="MSERAAHQVIKGYELTERIGSGEFGAVYRAYQYTVGREVAVKVI
                     LPAFANHPDFIRRFEMEAQLVARLEHLHIVPLYDYWRDPSGAYLVMRWLHGGNLQERL
                     QNYPLDLDSILNLLDQIASGLSVAHRKEIVHRNLKPSNILFDEQGNAYLVDFSIARDF
                     YQSANSLEKSQWIVGSPCYLSPEQISGELVSPQSDIYSLGVLLYEMLSGAHPFPGLAP
                     GEQFYRILNDPLPPIETLSLEVQEGVNAVIQKATEKNPRHRYRDVLEMAAALHQAASL
                     AERETEAISAEQLTLREAEILGLIVAGHSNKQIAQKLFVELSTIKWHITQVYNKLQVR
                     TRYQAILRAREMQLIFPEQEREADDVLSESLDGAIDLTGVGLTNPYKGLRPFETADSR
                     DFYGREDMVEKLVGRLAESEPYARFLAIIGPSGCGKSSLVKAGLIPALRRGALDGSES
                     WFIVEMEPGTQPVDNLEIALTRVAADDSVNIHAQLEHDKFGLLRVAGLILPPDNSQLL
                     ICIDQFEEVFSLIEAEALRSRFLDLLTVAVSDPNSRVRILLTLRADYYDRPLHYPDFG
                     ELVRRRMETVLPLNAQEMERAILLPAQRLGVKFEPGLAASIIEEVMYQPGALPLLQYA
                     MTELFDRREDGRLTRQAYQAIGGAVGALAKRAEELYCQLDTESQQAARQAFLQLVAMG
                     DAEDGKAIASDTRRRAIRADLLTSTYDAEALDEMIDTYAAYRLITLDIDHESHRPTVA
                     LAHEALIREWERLRTWLKDSREDLLQHRRLSALAREWREAGQDPAYLLRQTRLDQFSA
                     WAAQTDLRLNPQEKAFLEASQTERQARRAAEANRQAREVALEQRARRFLRALVAVLLL
                     ATLGSLALAWNARKQADLSTSRELAVAAINNLEVDPERSILLSLQALRTVDTREAKDA
                     MHRSLQTSRVRMALIEHTGWVRSVEFSPDVKTIATTSYAWEATIWDTGSGQVLFSLPG
                     TVARYSPDGVRLATGSLDGTLTIWNLATRNRLWTKRGHTWRIFDVHFSPDGELLVSSS
                     VDNTFIVWDTDTGQEIFSSTAKVEGFDALNNVAFSPDGRLLISVDITESGNKISFWGV
                     DRDWALLNQLPSNDKMIFSPDGRWLVSTGAGTHTNAIVLWDISNANLESLDLSSLKPL
                     VVSEAHTSMINNFAFSPDGSVLASVGQDGLAKVWRPSSEGLELLMVLSGHTDQLTDVA
                     FSPDGTRLATSSADGSVRIWDITPAGASDWFTLAAHQGPIYLFDLTADGKYLATASSD
                     GVAKVWDLASGRELLAITNHGGPLYGVAISPDGTLLATAGDDNIAKIWKLRLSPGAAT
                     VELLHTLSGHIKSWLVNHKFPGLTSVAFSPDGAKLATGGVDGVAKIWEVSTGQELLSI
                     QTNPDRLGIVRLAFSPDGLLLATTNDAPYELAKIWDAASGKEISAFSGQRYIRFISGI
                     AISPDGERVATSSANGSLKIWDAKTGEELLNLVGHTGIVEGVDFSPDGKYLASAGHDC
                     TEDVCEKPPRLLEELATSVDLVEGSVRIWDASSGEELQVYTSPRGPLYDVAFTPDGKK
                     VIASGMGFVCGYIFDTQELIRLANSRLTRWFTLDECRQYLHQEKCPPR"
     gene            8969..9130
                     /locus_tag="AC16CB_06560"
     CDS             8969..9130
                     /locus_tag="AC16CB_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342165.1"
                     /translation="MPSTLSRWRGVIAAGLGIAAYTLLRTACNGWIELSNDGEKLRLA
                     AWGERQSRG"
     gene            complement(9162..9386)
                     /locus_tag="AC16CB_06565"
     CDS             complement(9162..9386)
                     /locus_tag="AC16CB_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342166.1"
                     /translation="MSTDDELRELLDGTAAEISRLSSNPRTWLSWMVYLLGRLEQQTT
                     DGSPSHKDLYKDMLSALLDAIRNRLRTGGW"
BASE COUNT         2124 a         2533 c         2604 g         2167 t
ORIGIN      
        1 tcatcgccaa cccggccgat gagcacatcg ccgccctgcc gggcatcttg gagcgcttcc
       61 cgccaaatca ggttgtgtgg gctggtccaa cgcacgcctc cagctccgcc cgttacctgc
      121 aatccacctt gtccgaggtt ggcatgcagc ccatccccgc ccagagcggg caaaccctgg
      181 atttaggcca gggtgcaacc ttgcagatcc tcagcgccaa cgcccgcggc gccgtgctgt
      241 tgttggaatg gcgggatttc tccgccctgc tgcccatggg cttggatacc gaaagcctgc
      301 aatccctgcc agatcgcttt gccccggtga ccgccctgct gctggcggaa ggcggctacg
      361 cgccgctcaa cccgccggag tggattgcca acctgcaccc ccaggtggtc ttactcagcg
      421 tctcccccgg cgaccatcat ggcctgccat cccctgagac actggaagct gtacaggatt
      481 acactctttt gcggacggac cgcaatggct ggatcgagct aaccacggat ggggagcaga
      541 tgtgggtgga ggtggaaaag aggtgattgg gcggtctgtt cgttgggaac gatttttggt
      601 caatgataga gcgggtgcgc atttccttgt gcacccgctc caatttcaga ttgcctggtc
      661 gaacgtgact agcgtaaata cagacaagct ggcgctattt ggtcctgcat tgtcccttat
      721 taaccagtaa ttcaaaaatg gttctggtcg cgtaagtctc gccagcgcgc agcaccgtgg
      781 agggaaaacc cggttggttg ggcgagtcgg gaaagtgctg ggtctccaaa gcgatgccgt
      841 cgccttgccg gtaggcgcga ccgcttgttc cgtagagtgt cccgtccagg aagtttccgg
      901 cgtagaactg aatgccaggc tcttctgtcc agacattcag cgtgcggcga gagcccggct
      961 cgcataaggt agctgccagg agcatggagg tgtcatcaaa tgagggccgg ctcagcaccc
     1021 agttgtggtc aaagcctcta ccgatggcga cctgctcagc gttagaacga aggccgtcag
     1081 aaattggttt cggcaccgtg aagtcgaacg gcgttccagc aacgggtgcg agttcgccgg
     1141 tggggatcag ggtggggtca accggcgtga agcggtctgc gttgaggtac aggatgtggt
     1201 cattgatgtc gccctcccct tctccagcca ggttccaata cgtgtggttg gtcagattaa
     1261 cgacggtagg tgcgtcggtg gttgccacgt aatccatgac gatctggttc ttattgttca
     1321 aggtgtaggt gacgtaaacg tcgaggttcc cggggtatcc cgcctcgccg tcggcgcatt
     1381 cggccggctc atagccctcg cccgccaggc tgagatagtg caactcgaga accactccgt
     1441 ctgggccgac ctccgctttc gtcacctccc agaccttctt gtcaaacccg acgtaaccgc
     1501 cgtgcaggga gttgggaggg ttattaatgt caaggcagta tgttacgccg tcgagggtga
     1561 acctcccttc atcgatgcgg ttggcatagc ggcctgttat acagccaaaa taggggttct
     1621 tggtttcata atcagagaga ttatcgaagc cgagggccac attctccata ttaccccatt
     1681 tgtcgggcac cttgatggag gtgatgatac cgccataggt gatgatcttc acctcaatcg
     1741 gcctcttgcc tgcgttcgtt aaagtatatt cgtgaacgtc ctggccaccg atcgtcgttc
     1801 cataaatcct ttcggaaata tggcctaggc ctttagcccc tgatgctggt cccaaggcga
     1861 gaagcatcga gaggacgagg acgctgatta agacaattcg ttttgagagt ttcatggtcg
     1921 ttatcctcct atcagtttta ctattcggat taaaagtctg tttgaattga tccgctcgat
     1981 cgcgttcaag ttaaatgaaa cgctctgagc tcaccaaatg ttttaggcaa tctgtgaatc
     2041 gtcgatatag acgagcgata aatgtggaaa cttgacgatt ttgctaccat ctacctcgtg
     2101 taatatcgag aatataccag gactattcac aatggagaat gcggctatct gatttaatat
     2161 acagcgaagt gaaatcacgc cgacagaatt gattatttaa gaaaatagac acctcctcct
     2221 gccttaacag aattggcggt gtcgaagttg tatatattga aatgatggat gatgatttgg
     2281 cgaggttatc ctgtcaaagc aggttattgc tgaattaaac aaataataac agagtcaact
     2341 ttacgagtca agcgagtttt cgttatatct ttgtgacaaa atcggtggat gaattagaaa
     2401 agtggtgaaa aggcaagaaa tccccatacc taaactttgg gcttagtaag gaaacaccgc
     2461 ttctagcctg gagcagtgaa atttatatcc cctacgaggt gatgcttatc ttccaagccg
     2521 gaagtatctt ttctggcaga ggtgttgctg gggggcatct gttccttgcg tgagcaagcc
     2581 ctagtagtga gctaccgctt gtatccctta tctgcaggta tatccctcta aaacgaaata
     2641 attttgaacg acatcacttc tatcacttgg acgatagaaa aatccctgga tttatagaat
     2701 aaattcaggt tgcctctttt tgtactgctg tctcataacc gctaatcccc gatcatacca
     2761 gagctccccc atagggcgac attgcaatgc agaacatatt tcaaagaatg actatattcg
     2821 ccagattaga tctccaccca accttgccaa cagcatctct acatacttgg ttactagagc
     2881 atagtatata gaaagcagag ataacctgaa agagggttcc catgaaagta cttgtcctcg
     2941 gtggtacggg cttatgtggc aaggatgggg ccccgctgct tgtgcagttg aggctgggtt
     3001 acgctacgga cttgttcatc gacatgacta aagccaccat ggagttcggt gtccggctga
     3061 cttcgcatga tgaatatata cagaaggtgc tagtaaatgc tccggtacac tgaagaataa
     3121 aatgagctag ttcataacac aagaaaaaat catgaataaa atcctcgtga gaaaaatgag
     3181 cgggacagtt agtgtcgtga ccgctcgtga aggaggggtt cgcaactggc agtgctgggc
     3241 gccgtacgcg acggtttcgt ggtcactggt ctacgccgcg ctaggggtct actgggggtt
     3301 gtgagcaggt actggttccc ctacgacccc acgcttgtgt ctgacgatac aacgttggtg
     3361 gtaggctggt tgggctcggt cgtcctgctc agcgtctctt ccggcgatcg ccagagcctg
     3421 cccaccctag agacactgga agtgatgcaa ggccataccc tgctgcgcac cgatcgcaat
     3481 ggctggatcg atccaaccat ggatggtgcg cagttgtggg tagaagtaga gaaacggcgt
     3541 tagggataga tcagattcaa ggcatcagcg ttccttgatc cgcaccagtt cttccaacct
     3601 agacggtcca ctgaggcgag cctcgacggg gagtaattca tccttgatgc gcgccagctc
     3661 ttccagcctc gatggccctg tcaagcgagc atccaccggg gatacctcgt ttttgaaccc
     3721 tgctaactcg tccagcctcg cccactgtgt ggcgcgagtc acgattgtag tgactagtaa
     3781 gatgctggcg gctaggatca gcacaatcac aaaccaattg atttttggtg caactgattg
     3841 ttggttcatg gttaactctc ctttgcttaa tttacaattg aacattacct tattcgattt
     3901 gtttcctcag cgaccctcag tcgccgtact tactcccagg atagcaggtc tcttgctctc
     3961 taggaagtca gggaaaaggc ggggatcaaa tcgccaaaat cagcccggac catccaaaaa
     4021 caccgcttgt taatctttgg caagaagggg ggtagggtaa aagtagggga tctaatgtgc
     4081 agttatcaat tactaaaaat tgagtttata atagtattag tttccgttca tcccgcgcgc
     4141 acttcgaaac aaccgcttcc ttagttgata taaaaactta tatgtcagaa agagcagccc
     4201 accaggtcat taagggctat gagctgactg aacggatcgg ctcaggcgaa ttcggcgcgg
     4261 tttaccgggc ctatcagtat acggttggtc gcgaggtggc tgtcaaggtc attctgcccg
     4321 ctttcgccaa ccaccctgac ttcatccgcc gctttgagat ggaagcccaa ctggtcgccc
     4381 ggctagagca cctgcacatt gtgccgctat acgactactg gcgcgacccc agcggggcat
     4441 acctggtgat gcgctggctg catggtggga acctgcaaga acgtcttcag aattatccgc
     4501 tcgacctgga ttcaatattg aacctgttgg accagatcgc ctcgggattg tcggtcgccc
     4561 atcgtaagga aatcgtgcac cgtaacttaa agccctccaa tatcttattc gatgagcagg
     4621 gtaacgctta tctggtcgac ttcagcatcg cccgcgattt ttaccaatcc gccaacagtc
     4681 tggagaaatc gcaatggata gtgggatccc cgtgttacct ctctccggaa cagatctcag
     4741 gcgagctggt ttcccctcaa tctgacatct attcattagg cgtgctgctc tacgaaatgc
     4801 tgagcggagc gcacccattc cctggtttag caccagggga acagttttat cgaattctca
     4861 atgatccgct tccgcccatc gaaaccctct ccctggaagt ccaggaaggg gtgaatgccg
     4921 tcatccagaa ggctaccgaa aagaacccgc gccaccgcta tcgtgacgtt ctcgagatgg
     4981 ctgctgccct gcaccaggca gccagcctgg ccgaacgtga gacggaggcc atttctgcag
     5041 agcagcttac cctgcgcgag gccgagattc ttggcctgat cgtcgccggg cattccaaca
     5101 agcagattgc ccagaagctg ttcgtcgagc tgtccaccat taagtggcat atcacccagg
     5161 tctacaacaa gctgcaggtg cgcacccgct accaggcgat tctgcgcgcc cgcgagatgc
     5221 agctcatctt ccctgaacaa gagcgagagg cagatgacgt tttaagtgaa tcgttggatg
     5281 gggcgattga cctgaccggg gtaggcttga ctaatccata taaaggtctg cgcccatttg
     5341 aaaccgccga cagccgggat ttctatgggc gggaggatat ggtcgaaaaa ctggttgggc
     5401 ggctggcgga aagcgagcct tacgcccgct ttctggctat tatcggcccg agcgggtgcg
     5461 gcaaatcgag cctggttaaa gcaggcctga tcccggcctt gcggcgcggt gcgttggatg
     5521 gttcggaaag ctggttcatc gttgaaatgg aaccgggcac ccagccagtt gacaatttag
     5581 aaatcgccct gacccgggta gcagcggacg attcggtgaa tatacacgcc caactcgagc
     5641 atgacaaatt tggcctgctg cgcgtcgccg ggctgatctt accacccgat aacagccagt
     5701 tgctgatctg catcgaccag tttgaagagg tattctcact tatcgaggca gaagcgttgc
     5761 gtagccgatt tctggattta ctgaccgtag cggtctccga tcccaatagc cgggtgcgaa
     5821 tcctactgac attgcgggca gattattacg accgccccct ccattacccc gacttcggcg
     5881 agctggtgcg ccgccgcatg gagacggtct tgccacttaa tgcccaggag atggaacgcg
     5941 ctatcctgct cccggcgcag cggctcgggg ttaagtttga acccggtctg gccgccagta
     6001 tcatcgagga ggtcatgtac caacccgggg ctttaccctt gctacagtat gccatgacgg
     6061 aattatttga ccgccgtgag gatggccggc tgaccaggca ggcttaccag gcaatcggag
     6121 gcgcggtcgg cgccctggcc aagcgagcgg aagaactcta ttgccagctg gacacagaaa
     6181 gccagcaagc tgcccggcag gccttcctcc agctggttgc catgggtgat gcggaggacg
     6241 gcaaagcgat agcatccgac acccgccggc gcgccatccg ggcggacctg ctcaccagca
     6301 cctacgatgc cgaggcgctg gacgagatga tcgacaccta cgctgcctac cgattgatta
     6361 ccctggatat tgaccacgag agccatcgac ctaccgttgc cctggcccac gaagccctga
     6421 tccgggaatg ggagcgattg cgcacctggc tcaaggacag ccgggaagac cttctccagc
     6481 accggcggct gagcgccctg gcccgtgaat ggcgagaggc aggccaggat cccgcctacc
     6541 tgttgcgcca gacgcgcctg gaccagtttt ccgcctgggc agctcagaca gacctgaggt
     6601 taaatccgca ggagaaagcc ttcctggaag ccagccagac tgagcggcag gcccggcgtg
     6661 ctgccgaagc caaccgacaa gcgcgcgagg tcgccctaga gcaacgggcg cgccgctttc
     6721 tacgagccct ggtggccgtc ttgctcctgg ctaccctggg ttccctggcg ttggcctgga
     6781 acgctcgaaa gcaggctgat ctgtcgacct cccgcgagct ggctgttgcg gcgatcaaca
     6841 acctggaggt cgatccagag cgcagcatct tattgtccct gcaagccctg cgtacggtcg
     6901 atacccgcga agcgaaagac gccatgcatc gctccctcca aacatcgcga gtgagaatgg
     6961 cattgatcga gcataccgga tgggtgcgct cagtcgagtt cagcccagat gtgaagacca
     7021 tcgccacaac cagctatgca tgggaagcga ccatctggga tacgggtagc gggcaggtgc
     7081 ttttcagcct gcccggaact gttgcgcgct atagcccaga cggagtacgc ttggctaccg
     7141 gcagcctgga tggcacgctc acgatttgga atcttgccac ccggaacagg ctgtggacaa
     7201 aaagaggtca tacctggagg attttcgatg tgcatttcag cccagatggc gagcttttag
     7261 tttcctccag cgtggataac acctttattg tctgggatac agataccggt caagagatct
     7321 tctcctcgac cgcaaaggtc gaaggattcg atgctctgaa taatgtggct tttagcccgg
     7381 atggcaggct gctgatctcc gtggacatta cggaatctgg aaataagatc agtttttggg
     7441 gagtagaccg tgattgggcc ctgctaaacc aactgccgag taacgataaa atgatcttca
     7501 gcccggatgg caggtggctg gtttcgacgg gagcgggaac tcataccaac gcgattgtct
     7561 tgtgggatat ctccaatgca aacctcgagt ctctggattt atcgtcattg aagccactcg
     7621 tagtatctga agcccacaca tccatgatta acaattttgc ttttagcccg gacggatccg
     7681 tactggcttc ggtcggtcaa gacggtctgg ctaaggtgtg gcgtccctct tctgaaggtc
     7741 tggaactgct catggtttta tccggacata ctgatcagct aactgacgtt gcctttagtc
     7801 ccgatggcac acgattggct accagcagcg cagatggcag cgtgcgcatc tgggatatca
     7861 ctcccgcagg cgcctcggat tggttcacat tagccgctca tcagggtcct atctatcttt
     7921 ttgacctcac ggctgatggg aagtacctgg ccacagccag ctcagatggg gttgccaaag
     7981 tgtgggacct ggcttcagga agggagctgc tcgccatcac caaccatggc ggtccgctgt
     8041 acggtgtggc catcagcccc gatggcaccc tgctggcgac cgcaggagat gataatatcg
     8101 ccaaaatctg gaaacttcgc ctctctccgg gggcggccac ggtggagctg ctgcacactt
     8161 tgagcgggca catcaaaagc tggctagtca accataaatt ccccggactc acttcggtcg
     8221 ccttcagccc ggatggcgcg aaacttgcca cgggcggcgt ggatggagtg gcgaagatct
     8281 gggaagtgag cacaggccag gagctgcttt ccatccagac aaatccagat cgtttgggca
     8341 ttgtccggct tgctttcagt ccggatggcc tccttctagc aacaaccaat gatgcgcctt
     8401 atgaattggc gaaaatctgg gatgcggcga gcggcaagga gatatccgct ttttcaggtc
     8461 agagatatat cagattcatt tcgggcatag ccattagccc ggatggggag cgcgtagcaa
     8521 cgagttcagc caatggcagc ctaaagattt gggatgcgaa aaccggcgaa gagttgctaa
     8581 atctcgttgg ccataccggc atcgttgaag gcgtggattt cagcccggac gggaaatacc
     8641 tggcctcagc cggccatgat tgtaccgaag atgtgtgtga aaagcctccg aggttattgg
     8701 aggaactcgc aacttctgtc gacctggtgg agggcagtgt cagaatatgg gatgcatctt
     8761 cgggggagga gctacaggtg tataccagcc cgagaggacc cctctacgac gtggccttca
     8821 ccccagacgg gaagaaggta atcgcttccg gcatggggtt tgtctgtggc tatattttcg
     8881 acacgcagga attgattcgc ctggccaatt ctcgtctcac gcgctggttc acgttagatg
     8941 agtgccgcca gtaccttcac caggaaaaat gccctccacg ctgagccgct ggcgcggcgt
     9001 tatcgcagcc ggcctcggca tcgccgccta taccctgctg cgcaccgcgt gcaatgggtg
     9061 gattgagcta tccaatgatg gggagaaact gaggcttgcg gcttggggtg agagacaatc
     9121 gcggggataa tcctccttca aaaacggcgt atcctgtact ttcaccaccc tccagttctt
     9181 agtcgatttc tgatcgcatc cagcaaagca gatagcatat ccttgtataa gtccttgtga
     9241 ctggggcttc catctgtagt ttgttgctcc agacgcccaa gcaaataaac catccacgat
     9301 aaccaggtgc gcgggttact ggatagtcgt gatatttccg ctgcagtccc atccaacaat
     9361 tcgcgaagtt cgtcatctgt actcatatat tatttccttc ttgaccagat cccctttcag
     9421 gctagggg
//
LOCUS       DBNGSX010000216         4062 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1540434, whole genome shotgun sequence.
ACCESSION   DBNGSX010000216 DBNGSX010000000
VERSION     DBNGSX010000216.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1540434"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="AC16CB_06570"
     CDS             <1..735
                     /locus_tag="AC16CB_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342167.1"
                     /translation="FGVAALLLQRLVPLEWIAAESYLSQSGYEGLTMPWIIFYAAILR
                     PSFPLLLIVLVGWVYTLINLAQTYFFYCSRIIFAWSFDRLIPEKVCYIHPTLNSPVIA
                     IAIISVIAEIGVIDAALGGVMGAQLNFVFFAVCTQLVPVLSMTLFPYLKPDLFENSSA
                     FVRRKLGNIPVITIVGTITMTYLLWMIIASFAFPAVGGRIGTGTLGTLAGFIISGIVV
                     FYIARAYRMRSEGIDIKWTFQSIPPI"
     gene            831..1811
                     /locus_tag="AC16CB_06575"
     CDS             831..1811
                     /locus_tag="AC16CB_06575"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3342168.1"
                     /translation="MKFGSLFRGSKITRILYGSDFHGSEAVFRKFLSAALQYQASYLI
                     VGGDVTGKAMIPIIHQGNGRYVGYVFGRKEEPSTPEELDKLKKTISNVGFYPIVVEKD
                     EAQALESNKELMSQRFEEEMCQRVRDWMVLAEQTLVPQKKTLYFMPGNDDLYSVDAVI
                     DEFEHVFNPDMKRFIIDDDYELYGLSNANMTPWACARDVEEDELMLKLGALEELVTNP
                     ERAILALHVPPYASNLDTCPELDKDLKMITLGGQVVMKSAGSTAVKAFIEKVQPMLTL
                     HGHIHESPGYTRIGRTLAINAGSEYAEGILKAAIINLEGGKVKGHLLISG"
     gene            1813..2484
                     /locus_tag="AC16CB_06580"
     CDS             1813..2484
                     /locus_tag="AC16CB_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342169.1"
                     /translation="MGKFGDIQEDFYAERTRILEALQEPENRHVIMRLIGALAFRTHC
                     PQFGHIQDELGRKFTDIDFASYRRFIKDIRRLLSELGYLEEKMVTQLFGDARLLFNDP
                     VYDRHIDIFFEQLDFCHPVSFSGRLEVEELTLPLAELLLEKMQIVQINEKDLIDSIML
                     LREHPIGEGDRETVNAEVIASTLGDDWGFWRTVTGNLNLLDQMLDQYTGLKDEDRQVV
                     RERIH"
     gene            2651..>4062
                     /locus_tag="AC16CB_06585"
     CDS             2651..>4062
                     /locus_tag="AC16CB_06585"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF012589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIR3342170.1"
                     /translation="MTPTKKYILAHDTGTGGDKAVITDLQGRVIHSAYQSYGLSYPQA
                     EWVEQDPDELWRTVAATTREVIRQSGVDPAEILGVGISAQMFNALPVDEQGQPLTPML
                     SWLDLRSIRQADRLFQGDLPAFLFQHTGNMPTAKDVIPKILWLKEERPDIWERTAYLF
                     DCKEYIIYKLTGKVATDWVGASCYFLFDPHQRSWSPKACARLGIPLDKLPPAYPCTDV
                     IGEVTEEAAKLTGLLPGTPVVNCGGDVATAQSGAGANRDGKAHLCIGTATWVGISTSE
                     FKNDPEKPLWGLSHLHPKKWVIAGEMETGGGALMWFRDTLFGEQAGQAGGELSTYEMI
                     SKLAQSLEPGSDKLIFLPWLSGERAPVLDHYARGGFVGLTLSHNMAHMARAVMEGVAY
                     HIRWICETLDRLGFQFEEVNAIGGGCKSEIWPQIISDVTGRELHIVEHPLEAGAKAAA
                     LTVAVGMGVYASMDEIDDLI"
BASE COUNT          915 a         1006 c         1160 g          981 t
ORIGIN      
        1 tttggagtgg ccgcccttct gttgcagaga ttggtgccgc tggagtggat cgctgcggag
       61 agctacctga gccagtccgg ttatgaaggc ctgacgatgc cctggatcat cttctatgcc
      121 gccattctca ggcctagctt cccgttgttg cttatcgtgc tggtgggttg ggtgtacacg
      181 ctgatcaacc tggcgcaaac ttatttcttc tattgcagcc ggatcatttt tgcctggtcc
      241 tttgaccggc taatcccgga gaaggtttgc tatatccacc ccacgctgaa tagcccggtc
      301 atcgccatcg ccatcatctc ggtgatcgcc gagataggtg tgatcgatgc ggcgttgggc
      361 ggggtgatgg gtgcccagct caactttgtg ttctttgccg tgtgtacgca actggttccg
      421 gtactatcga tgacgctctt cccttacctg aaaccggatc tgttcgagaa ctccagcgcc
      481 tttgtacggc gcaagctggg caatatcccg gttattacca tcgtagggac gatcacgatg
      541 acctacttgc tgtggatgat cattgcttcc tttgctttcc cagcggtggg tgggcgtatc
      601 ggcacgggta ctttgggtac gctagccggg ttcatcatca gcggaatcgt cgtcttctat
      661 atcgcacgtg cttaccgcat gcgatcggag ggcatcgata tcaaatggac cttccagtcc
      721 atcccgccca tatagacttg gcgcttaccc tcacctaatc tggctttccc ctctccacgg
      781 gggagagggg aagtcaggtg ggggcgaaag aacttatacg gagtatatta atgaaatttg
      841 gtagcctgtt tcgcgggtcg aaaattacgc gtatcctgta tggttccgac ttccatggat
      901 ctgaggcggt ttttcgcaag ttcctctcag cggcacttca gtatcaagcc agctacctga
      961 tcgttggtgg agatgtcact ggcaaagcga tgattcccat catccaccag ggaaatggtc
     1021 gctacgtggg ctatgtcttt ggtcgcaaag aagaaccgtc cacgccggaa gagcttgata
     1081 aacttaaaaa gaccatcagc aatgttggtt tttatcccat cgtggttgag aaagacgagg
     1141 cgcaagcgct ggaatcgaac aaggagctta tgagccagcg ttttgaggag gagatgtgcc
     1201 agagagtgcg agattggatg gtgctggctg agcagacact cgtgcctcaa aagaaaaccc
     1261 tctatttcat gcctggtaat gatgatctat actctgttga cgcagtcatc gatgagtttg
     1321 agcatgtctt caacccggac atgaagcgat ttattattga tgatgactat gaattgtatg
     1381 gcctctcgaa tgccaatatg accccctggg cttgcgctcg tgatgtagag gaggacgagc
     1441 tgatgttgaa gctgggtgcg ctggaagaat tggttaccaa cccagagcga gctatccttg
     1501 ccctacacgt acctccttat gcttcgaacc tggatacctg cccggagctg gataaagatt
     1561 tgaagatgat cacactggga ggtcaggtgg tgatgaagtc ggctggctcc accgcggtga
     1621 aagcctttat tgagaaagtg caacccatgc tgaccctcca tgggcatata catgaatctc
     1681 cgggatatac acgtatcggt cgcacgttgg cgatcaacgc tggcagcgaa tacgcggagg
     1741 gtatcttaaa agcagctatc attaacctgg agggtggcaa ggtcaaggga cacttgctga
     1801 tttctggata atatgggtaa attcggagac atccaagagg acttctacgc cgagcgcacg
     1861 cgtatcctgg aggcgctcca ggaaccagaa aaccggcacg tcatcatgcg cctgattggg
     1921 gcgttggctt tccgcaccca ttgtccgcaa tttggtcata tccaggatga actgggacgc
     1981 aagtttaccg atatcgattt tgcttcttac cgccgcttta taaaggatat ccgccgcctg
     2041 ctcagcgagt tgggctacct tgaagagaaa atggttaccc agctctttgg cgatgcgcgc
     2101 ctgcttttca atgaccctgt atatgacagg cacatcgata ttttcttcga acaacttgat
     2161 ttctgtcacc cggttagctt ctccggtcga ttggaggtgg aggaactgac acttcctctg
     2221 gctgaactgc tgctggagaa aatgcagatc gtacaaatca atgagaagga cttgattgat
     2281 tctattatgc tcttacgcga gcatcctatt ggcgaaggtg accgggaaac ggtaaatgct
     2341 gaggtgattg cttctaccct aggtgatgac tggggcttct ggcgcactgt cactggcaat
     2401 ctaaacctgc tggaccagat gctggatcaa tacactggtc taaaagacga ggaccgccag
     2461 gtcgtgcgag aacgcataca ctagttgcag gtgcgcattg aaagccagcc gaagacggta
     2521 aaatggaagc tgcgcagccg aattggcgag cgagtgaagt ggtacaaaga tgtagaggaa
     2581 ttgatgcacc gctgacacgc ttcgccggat atttgaaaat gatcgattgt ggcatactta
     2641 aggattctca atgacgccca caaagaaata catcctggcg cacgacactg gaaccggggg
     2701 tgacaaagct gtcatcaccg accttcaggg tcgggtgatt cactcggcat accagtcgta
     2761 cggcttgagc tatccccagg cagagtgggt ggagcaggat cctgacgagc tgtggcgcac
     2821 cgtggctgcc acaactcgag aggttatccg gcaatcgggt gtggacccgg cggagattct
     2881 aggggtgggc atatctgccc aaatgttcaa cgccttgcct gtggatgagc aaggccaacc
     2941 gctgacacca atgttgagct ggctggacct gcgcagcatc cgccaggctg atcgcctgtt
     3001 tcaaggtgac ttgcctgcct ttctcttcca gcatactggc aacatgccca ccgccaaaga
     3061 tgtgattcct aaaatattat ggctcaaaga ggagagacca gacatctggg agcgcaccgc
     3121 ttatcttttt gactgtaaag aatacattat ttataaactg accggaaaag tagccaccga
     3181 ctgggtgggg gcttcctgtt acttcttgtt cgatccgcac cagcgcagct ggtcgccgaa
     3241 ggcctgcgct cgcctcggca ttccactgga taagctgccg cccgcctacc cctgtactga
     3301 tgtaatcggg gaggttactg aagaggcggc aaagctgacc ggcttattac ctggcacacc
     3361 ggtagttaac tgtggcggag atgtggctac agctcaatct ggggcaggtg cgaaccggga
     3421 tgggaaagcg cacttgtgca ttggcactgc cacttgggtg ggtatctcaa cctccgaatt
     3481 taaaaatgac cccgaaaagc cattgtgggg gctgagccac ctgcatccca agaaatgggt
     3541 cattgcaggt gagatggaaa ctgggggcgg tgcgctgatg tggtttcggg atacgctttt
     3601 tggggagcag gctggtcaag ctggcggcga gctttcaacc tacgagatga tcagcaagct
     3661 cgcccaatcc ctggaaccgg gctctgataa gctgattttc ttgccatggc tctccggaga
     3721 gcgagctccg gtgctggacc attatgcccg gggcggcttt gtcggattga ccctgagcca
     3781 caacatggct cacatggccc gcgccgtgat ggaaggcgta gcctaccata tccgctggat
     3841 ctgcgagacc ttggatcgtc tgggctttca attcgaggaa gtcaacgcca ttggtggtgg
     3901 ctgtaagagt gaaatttggc cgcagatcat cagcgatgtc accgggcgtg agctccacat
     3961 tgtggagcac ccgctggagg cgggtgccaa ggctgctgcg ctgactgtgg cggtggggat
     4021 gggggtatac gccagcatgg atgaaatcga tgatttgatc ta
//
LOCUS       DBNGSX010000217         7394 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_987271, whole genome shotgun sequence.
ACCESSION   DBNGSX010000217 DBNGSX010000000
VERSION     DBNGSX010000217.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7394)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7394)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7394
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_987271"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..4225
                     /locus_tag="AC16CB_06590"
     CDS             185..4225
                     /locus_tag="AC16CB_06590"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR3342171.1"
                     /translation="MSFVHLHVHSEYSLLDGFSKVKKLVKRAKEMEMPALALTDHGTM
                     FGVIDFYNAATAAGIKPIIGMEAYLAARSMQERDPQEDKKSTHLLLLAENETGYKNLL
                     QIASAAQMQGFYYFPRIDHDFLAQHSEGLICTSGCMAAEIPRLIYQSNLEAARRQLDW
                     YYEVFGPERFFLELQSHQIQELDTINQHLMTLGKRYKARFVATNDVHYVNPQDARLQD
                     ILLAIQTSCVLTDPNRMRMSDTSYYLRSPQEMAQLFAEVPEAIHNTVLIAERCNVDLG
                     FKGYHLPVFEVPEGQTPVSYLRQQCEKGFVKRFGSRADNPVYRQRLEYELDIIHQMGF
                     DTYFLIVWDLCQFARDQGIWYNARGSAAGSIVAYALEITLVDPIEHGLIFERFLNPGR
                     ISMPDIDLDFRDDRRAEIMEYATKKYGEDKVAQIITFGTLGARAAIRDVGRVMDIPLN
                     EVDNVAKLIPNIPGKPMTISDALEEVPDFKKMYDTAPYLRDLIDTATEMEGVVRNAGT
                     HAAGVIITDKPLLEYIPLHRPTGSASSEDSPVKVVTQFEMSVLESLGLLKVDFLGLAT
                     LTIMARACDLILQRHGVELNLDNIPLDDPATYELMGRGETAGVFQVEGSGMRRYLIQM
                     KPKELANVIAMVALFRPGPMDFIPGYIRRMHGEEPVTYRHPALEPIFQETYGYPVYQE
                     QLMFAVMQLAGYTAPEADDLRKAVAKKQKEKLLKHRQKFVKGAVKNSVPEETAEAIFD
                     EWEEFARYGFNKAHAADYGIIAVQTAYLKAHYPVEYMTAVLSVNLSDTAKVALYVADC
                     RRLGIAIEPPDVNFSGWDFTIEDHPENSTSTIRFGLGAVKNVGHGPVDAILQGRDDQT
                     FVDLDDFAHRVDLRQVGKRALESLIKVGALDSFGPRPALLEGIDRIISVSAAHFRAAD
                     MGQMSLFGVHTGVSETISLPQLVGEISRREMLNWERELIGLYVSDHPLSPVMNEITQA
                     VTHFSAQLPEVAANEKVRVAGVIVRIRPHQSKAGKPMGFVAIEDLQGTIELVIFPSVW
                     SRVSTIIEYDRIILVDGRVDSSGAEPKVLVDNVTTDLNMKVAVQEDKASRRNNETTNR
                     QARKLEDRKIDAKEESLSEEDLLLESDSSNSSFNDFPDFDDDVPPMPDPPSDWDIYEP
                     EEPSIAAIAVGLFAGGENGELGVDTATVSDVMSDGNVQDAATGDQLVGEQALSVEEAT
                     EQKGMPGETPSTQDVKPDHAPEKTTEQATERLPAKPSDPAVLLPPFLVRPPISSAREQ
                     VQMITVILRSTGDKTRDVLRLRRIHGTLMSYPGNDRFAFHVFERERGYLLEFPNFTTD
                     VSAELIAKLQALVGADNIRLESITFQ"
     gene            complement(4399..5601)
                     /locus_tag="AC16CB_06595"
     CDS             complement(4399..5601)
                     /locus_tag="AC16CB_06595"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008443 - phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR3342172.1"
                     /translation="MANVQKKKGIIGILTGGGDVPGLNPAIRAVTFRALREGYDVIGI
                     RRGWGGLIDMIRDPKADNSNNFQILSEEIVNRAGRTGGTFLHTSRTRPSRISKPNVPE
                     HLRDTYTDEVNDLTPEVLKNIEYLNINYLIPIGGDDTLSYAVRLYQEGMKVVAIPKTM
                     DNDVPGTDYCIGFSTCVTRTLQMTNSLRTSAGSHERILVLEVFGRYAGFTALLPTMAG
                     AAHRCVIPEYKFKIDHLTELLVYDRLKNPSRYSVVLVSEGAMFEGGEMVFQDRTTDAY
                     GHAKLGGIGDLVSEKIKEIAPQFNNGKPINTITQKLGYMVRGGEPDAIDAIVPMAYGN
                     LALDLILNRIHGRMVVLKNGRYDNVPIDVVIAMKKAVNVKKFYNTERLRPNFEQFEMM
                     PLFIMTSD"
     gene            complement(5675..7354)
                     /locus_tag="AC16CB_06600"
     CDS             complement(5675..7354)
                     /locus_tag="AC16CB_06600"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA mutase large subunit family protein"
                     /protein_id="HIR3342173.1"
                     /translation="MYDRQKLAKLKQELEKWEETSLQRSLSQLPERNDRYMTTSSEDI
                     ERLYTPLDVAEMDYLRDLGLPGEYPYTRGVHPTMYRSRLWTMRMFAGFGTAEETNSRF
                     KYLLNQGQTGLSIAFDLPTLMGYDTDDHEALGEFGKCGVAVSSLKDMEFLLQGIPLDK
                     ISSSMTINSPAAIIWAMYIAVAEKQGVRPDQLRGTTQNDILKEYIAQKEFIFPPEPSM
                     RLVVDTIQFGTTTVPQWNTISISGYHIREAGSTAAQELAFTLADGLEYVRWGLERGLE
                     IDQFAPRLSFFFNAHNDFFEEISKYRAARRIWARELQQTFGAQNPRSWLMRFHTQTAG
                     VSLTAQQPENNIVRVAIQALAAILGGTQSLHTNSMDEALALPSEHAVTIALRTQQIIS
                     EESGVANTIDPLGGSFFVEAQTNQMEKQTYDYFRRIEDLGGVLPAIEKGFFQSEIADA
                     AYRYQREIDEGLRSIVGVNAFQDEKSVTIPILQMDPQGYRRQVARLEEVRRTRDSGQV
                     GQALDRLRIACQGTENTMPYILDAVRAYATLGEIVSVMKEVFGTYQEPSWI"
BASE COUNT         1661 a         1918 c         2020 g         1795 t
ORIGIN      
        1 cccttatcct ggcgaaattt taaggttggt taaacctccg ttctgcactc gccgtgctat
       61 aatctctcca agcccggaaa tcctttcacg gctagatttt ttttgccctg cgtgcatctc
      121 gcaaaccaat tcatatccat tagggttttg ttcgtttcgt tttgttggct gaccactgca
      181 aatcatgtcc ttcgtccacc tgcatgtcca ttccgaatac tccctgctgg atggcttctc
      241 caaggtgaag aagctggtca agcgcgccaa ggagatggaa atgcccgccc tggcgctcac
      301 tgaccacggt accatgttcg gcgtgatcga cttttataac gccgctactg ccgctggcat
      361 caagcctatc atcgggatgg aggcctacct ggctgccagg tcgatgcagg agcgcgaccc
      421 tcaggaggat aaaaaatcca ctcacctgtt gcttctggca gaaaatgaga ctggctacaa
      481 gaatctgctc cagattgcca gcgcggccca gatgcagggt ttttattact ttccgcgtat
      541 cgatcatgat ttcctggcgc agcactccga gggtttaatc tgcacttctg ggtgcatggc
      601 agccgaaata ccgcgcttga tctatcaatc taacctggaa gcagcccgcc gccagttgga
      661 ctggtattac gaggtcttcg gcccggagcg ctttttcctg gagcttcaaa gccaccaaat
      721 tcaagagctg gacaccatta accagcacct gatgacattg ggcaagcgct acaaagcgcg
      781 cttcgttgcc actaatgatg tccattatgt aaatccacaa gatgcccgtc tgcaggatat
      841 cctgctggca atccagacca gttgcgtgct caccgatcca aaccgcatgc gcatgagtga
      901 tactagctat tacctgcgta gcccgcaaga aatggcacag cttttcgccg aggtgccgga
      961 ggcaattcac aatacggttc tgatcgcaga gcggtgcaat gttgatctgg gtttcaaggg
     1021 ctaccacctg cccgttttcg aggtacctga aggtcaaacc cctgtatcct acctgcgtca
     1081 gcagtgcgag aagggttttg tcaagcgatt cggctcgcgt gctgataacc cggtctatcg
     1141 ccagcgcttg gaatatgagc tggatattat tcaccagatg ggcttcgata cgtacttttt
     1201 gatcgtttgg gatctatgcc agttcgcccg agaccagggt atttggtata acgcccgcgg
     1261 ctcagcagcc ggatcgattg tagcttatgc cctcgaaatc accctggtgg atcccattga
     1321 gcatggcttg atcttcgagc gctttctcaa cccagggcgt atttccatgc ccgatattga
     1381 cctggatttt cgcgatgacc gccgggcgga gatcatggag tatgccacaa aaaagtatgg
     1441 cgaggataag gtggcgcaga tcatcacgtt tggcacactg ggtgcccgcg ccgccatacg
     1501 cgatgtgggt cgcgtgatgg atattcccct gaatgaggtg gataacgttg ccaagctgat
     1561 ccctaatatc ccgggcaagc cgatgaccat ttcagatgcg ttggaagaag tccccgattt
     1621 caagaaaatg tacgacacag ctccctatct gcgcgatttg atcgatacag ccactgaaat
     1681 ggagggggtg gtgcgtaacg cgggcacgca cgcggccgga gtgatcatca ccgataagcc
     1741 cttgctcgag tatatcccgc tccaccggcc taccgggagc gcatccagcg aagacagccc
     1801 ggtgaaagtg gtcacccaat ttgagatgtc ggtgctcgag tcgctggggc tgctcaaggt
     1861 ggatttcttg ggattagcca cgcttaccat catggcacgc gcttgcgacc tgatcctcca
     1921 gcgacacggg gtggaactga acctggataa tatcccgctg gatgatcctg cgacgtatga
     1981 gctgatgggc aggggtgaga ctgccggtgt gttccaggtg gaagggtcgg ggatgcgccg
     2041 ttacttgatt cagatgaaac ccaaagagct tgctaacgtg atcgccatgg tagctttatt
     2101 tcgtcctgga ccgatggatt tcatccctgg ctacatccgc cgcatgcatg gggaagagcc
     2161 tgtgacctat cgccaccctg cactagagcc aattttccag gagacgtatg gatatccagt
     2221 ctaccaggaa cagttgatgt tcgctgtaat gcagctggcg ggttacactg ccccagaggc
     2281 cgacgacttg cgcaaagcgg tcgccaagaa gcagaaagag aagctgctta aacatcgcca
     2341 gaagtttgta aagggggcag ttaaaaacag cgtccctgaa gagacagctg aagccatatt
     2401 cgacgagtgg gaagaatttg ctcgatatgg gttcaacaaa gcccacgcgg ctgattatgg
     2461 cattattgcg gttcaaaccg cttacctgaa ggctcactac ccggttgagt atatgacggc
     2521 ggttctgtcg gtcaacctga gcgacactgc caaggtggct ctttatgtgg cggattgccg
     2581 ccgcttgggt attgccattg agccgccaga tgtcaacttt agcgggtggg atttcaccat
     2641 tgaagatcac cccgaaaaca gcacctcaac catccgtttt ggccttgggg cggtcaagaa
     2701 cgtggggcac ggcccggtgg atgcgatcct ccaggggcgt gatgatcaaa cctttgttga
     2761 tctggacgat ttcgcccatc gcgtggattt gcgccaggtc ggcaagcgcg ctttagagag
     2821 cttgatcaag gttggcgcgc tggacagctt tggcccgcgc cctgcccttt tggaaggcat
     2881 tgaccgcatc atctctgtca gcgcggccca ttttcgtgca gcagatatgg gccagatgag
     2941 cctttttggt gtgcacacag gtgtctctga gaccattagc ttgccgcaat tggttgggga
     3001 aatcagccgc cgagaaatgc tcaattggga gcgtgagttg atcggcctgt atgtctccga
     3061 tcatcccctc tctccggtga tgaacgaaat cacccaggcc gttacccatt tctcagctca
     3121 gctacctgag gtagcggcga atgaaaaagt acgggttgcg ggtgtgatcg tacgtattcg
     3181 ccctcatcaa agcaaggctg gcaagccaat gggctttgta gctatcgaag atttgcaggg
     3241 gactatcgaa ctggtcatct tccccagcgt ctggtcgaga gtctccacaa taatcgaata
     3301 tgatcgtatc attcttgtcg atgggcgcgt ggattctagc ggcgctgagc caaaggtgct
     3361 ggtggataat gtcaccactg atttaaatat gaaagtagct gtccaggagg ataaagcgag
     3421 tcggcgtaac aacgaaacga ctaatcgaca ggctcgaaag ttggaagatc ggaaaatcga
     3481 tgcgaaagaa gagagtctgt cagaagaaga tttactcctt gaaagtgact cttcgaattc
     3541 gtcattcaac gactttccag actttgacga tgatgtacct cccatgcctg acccgccttc
     3601 agactgggat atatacgagc ccgaagaacc aagcatagct gcgatcgcgg taggcctctt
     3661 tgctggtgga gagaacggag agttgggggt ggatacagcc acagtgagcg atgtcatgtc
     3721 tgatggaaat gtgcaggatg ctgctacagg agaccagttg gtcggggagc aagcgctctc
     3781 tgtggaggag gccactgagc agaagggtat gccgggtgag actccctcca cacaagatgt
     3841 aaagcccgac catgctcctg agaaaaccac tgaacaagca acggagcgac tgccagcgaa
     3901 accgtccgat cctgcggtgt tgctgccgcc tttcctggta cggcctccca tctcatctgc
     3961 acgagagcaa gtccagatga tcacggtgat actgcgttct acgggggaca agactcgcga
     4021 tgtgctgcgc ttgcgccgca tccacggcac gctcatgtcc tatccgggta atgatcgctt
     4081 cgctttccac gtatttgagc gcgagcgagg ctacctgtta gagttcccca actttacgac
     4141 ggacgtgagc gccgagttga ttgccaaact ccaggctctg gtgggcgccg ataacatccg
     4201 cttagaatca attacttttc aatagaagag aacaagtcgt tcccctccct tccgccaaag
     4261 cggcgggagg gggatgattt acggaaaaac gggctattgg gcggcattcc cgccgcccaa
     4321 tagcccgttt ttccgatata tttctcgctc tccagcgggg gagggcggca tcgccctgcg
     4381 ggcgtagccc taattgggtt agtcgctggt catgatgaag agcggcatca tctcaaattg
     4441 ctcaaagttt ggccgtaacc gctctgtgtt gtagaatttc ttcacgttca cggctttttt
     4501 catggcgatg acgacatcga tgggtacgtt atcgtaacgc ccatttttca agacgaccat
     4561 ccgcccatgg atacggttta gtatcagatc taaagccagg ttgccatacg ccatgggtac
     4621 gatggcatcg atagcatccg gctcgccacc gcgcaccatg taaccgagct tttgggttat
     4681 cgtattaatt ggtttgccat tattgaattg gggtgcaatc tctttgatct tctcagaaac
     4741 caaatcgcca attccaccca gtttggcgtg accgtaggca tcggttgtgc gatcctggaa
     4801 aaccatttcg cccccctcaa acatagcccc ttcggatacc agcacgactg aataccggct
     4861 agggtttttc aggcggtcat acaccaggag ttcggtcaag tggtcaattt tgaatttgta
     4921 ctctgggatc acacagcgat gagctgcccc cgccatcgtg gggagtaggg cggtgaaacc
     4981 agcatagcga ccgaatactt ccagcaccag gatgcgctca tgcgatccag ccgatgtgcg
     5041 taggctattg gtcatctgga gggtgcgggt gacacaggtg ctgaagccga tacaataatc
     5101 ggtgcctggg acatcattat ccatcgtttt gggtatggct actactttca tgccttcttg
     5161 atacaggcgt acagcgtagc tcaaggtgtc gtcacccccg attgggatga ggtaattgat
     5221 gttaaggtac tcaatattct ttaacacctc aggggtgaga tcattaacct cgtctgtgta
     5281 ggtgtccctt aagtgctctg gaacgttggg ttttgagata cggcttgggc gagtgcgcga
     5341 ggtgtgcaga aatgttccac ctgtcctacc tgcgcggttg acaatctctt cggagagaat
     5401 ctggaaattg ttgctgttgt ctgcttttgg gtcacggatc atatcgatga gaccacccca
     5461 cccgcgacgg atgccaatga cgtcatagcc ttcgcgcaac gctcggaaag tcacagccct
     5521 gatggctggg ttcaatccgg ggacatctcc accaccagtc aggataccaa taattccttt
     5581 tttcttctgt acatttgcca ttacaagacc tccaaatgct tataaaaaga tgcaggtcgt
     5641 tcgggaaaag cgggcggttg aacagcctga aatttcagat ccaggatggt tcttggtacg
     5701 tgccgaaaac ctctttcatc acgctgacga tttcaccgag cgtggcgtaa gcacgcacag
     5761 cgtctaggat gtagggcatg gtgttttcag taccctgaca ggcgatgcgc aagcgatcta
     5821 aagcctgccc cacttgcccc gagtcgcgcg tccggcgcac ttcttccagg cgagcaacct
     5881 gcctgcgata accctgagga tccatctgca agatgggtat agtcactgat ttttcatcct
     5941 ggaaggcgtt cacgcccaca atactgcgta agccttcatc aatttcgcgc tggtagcgat
     6001 aggcagcgtc agcaatctcg ctttggaaaa accccttttc gatagcaggc aacaccccac
     6061 ccaaatcttc aatgcgacgg aagtaatcat aggtttgctt ttccatttga ttggtttgcg
     6121 cttcgacgaa aaaggagccg cccaatggat caatggtatt cgccacgccc gattcttcgg
     6181 agatgatttg ctgcgttcga agggcaatgg ttacagcgtg ctcggaagga agtgccaggg
     6241 cttcatccat gctgttcgta tgcagcgatt gtgtgcctcc caggatagcc gccagcgcct
     6301 ggatggcaac gcgcacgatg ttgttctcgg gttgctgggc ggttaaggat acccccgcgg
     6361 tctgggtgtg gaagcgcatc aaccaggagc gtgggttctg agctccgaag gtctgttgca
     6421 actcgcgcgc ccaaatgcgc cgggcggcgc ggtattttga gatttcttcg aagaaatcgt
     6481 tgtgtgcatt aaagaagaaa gacaggcgcg gtgcgaactg gtcaatttcc agcccgcgct
     6541 ccaatcccca gcgcacatac tccaggccgt cggctaatgt gaaggccaat tcctgagcag
     6601 ccgtagatcc ggcttctcga atgtgatagc cggatatgct gatggtattc cattgcggta
     6661 cggtcgttgt gccgaattgg atagtatcca ccaccaggcg catggagggt tcgggaggga
     6721 agataaattc tttttgggcg atatattctt tgagaatatc attttgggtg gtgccgcgta
     6781 gttggtcagg gcgcactcct tgcttttctg cgacggcgat gtacattgcc cagatgattg
     6841 ccgccgggga gttgatggtc atgctggaag aaatcttatc cagagggatg ccttgcaaca
     6901 aaaattccat atctttaaga gacgagaccg ccacaccgca tttaccgaac tcacctagcg
     6961 cttcgtggtc atcggtatcg tagcccatca gtgtgggcag atcgaaggcg atcgacaggc
     7021 cggtttggcc ctggttgagc aaatacttga accgtgagtt agtctcctca gcagttccaa
     7081 atccagcaaa cattcgcatc gtccacaagc ggctgcggta cattgtcgga tgtacaccgc
     7141 gtgtataggg gtactcgccg ggaagcccca ggtcgcgcaa gtaatccatc tcggctacat
     7201 caagtggtgt gtagaggcgc tcgatgtctt ccgaagaggt tgtcatgtag cggtcgttgc
     7261 gttccggaag ctgtgatagg ctccgctgaa gggaggtttc ctcccacttc tctaattctt
     7321 gctttagctt cgctagtttc tggcggtcgt acatgctatc tccttcgctc tgtgacgatg
     7381 ttttaatcgc ttaa
//
LOCUS       DBNGSX010000218         3259 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1402069, whole genome shotgun sequence.
ACCESSION   DBNGSX010000218 DBNGSX010000000
VERSION     DBNGSX010000218.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1402069"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="AC16CB_06605"
     CDS             complement(<1..478)
                     /locus_tag="AC16CB_06605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3342174.1"
                     /translation="MISEEQLCAQIHDFDEHILAEVYDRYSPEIFRYTMRLLGDSELA
                     EECVAETFHRFLLAIKRGGGPRQYLRAYLYRMAHNWVSDYYRNQNKSALPLDPDCCID
                     EHKQPPHVFAEESMRRDVRLALSKLTPDQRQVIVLRFLEDWEYADISNALDKPVGAV"
     gene            551..1870
                     /locus_tag="AC16CB_06610"
     CDS             551..1870
                     /locus_tag="AC16CB_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR3342175.1"
                     /translation="MFRDKKIAASKQSTFARWISSLYSLLSGQGARQGYLAAVDQGVI
                     SASNFLATIILARSITPTELGVYGVGFIALRLVRSLQEGLVVQPMNVYGAGMDEHSFR
                     RYATSSSVLQISLAASSSLVAAISGWLLTALGNDTAGPALFSLWFAFLWWQLQEYIRR
                     MLYTRGMVFSAVVNTIFANAFRIFLLLWWAKQGKLTGILGLDAIAWGSLVALLPGLWA
                     TRSYWVRDFPNLKQTWLRNWGLGRWLLGSSLANWVAVEFYPVLTAGMISFAAAGAYRA
                     LQNLVAPIHLLLRATDTFLTPRAARLYELNGQTAMRRTLRLIYLISGIPILGVLAIAI
                     LFPTPLLRLLYGETYLAYSSSMALMGLFYALLYAYYPLQTAFKAARLTRPIFLANLGA
                     IAAMFTIGIWAILRWGVAGTIAGQTLNALIVSIILWGTWIRILRRQN"
     gene            complement(1915..3042)
                     /locus_tag="AC16CB_06615"
     CDS             complement(1915..3042)
                     /locus_tag="AC16CB_06615"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR3342176.1"
                     /translation="MGKGQIEESQSHSLHTGRQTITNASALFIAAFVLLFATWLYAQV
                     LWNEASWRNVIGRFILHALLTLILERALAITSFPRDWRYRAAFLLSIGVYLGYTGLQP
                     MNMAWVVNYINLSLVVLGSFSGGLLATGLNESLWEENSPPSQLIRMEVHQHHLNRIGD
                     PDPEPLLKRTFDVSLALLGLVLSLPVWLMGILVIWLEEPGPLLFVKNSVGKGGINFHQ
                     YKFRTMVREAEYSTGPVLASESDKRVLVVGRFLRKTALDELPQLINILLGQMSFVGPR
                     PQRTVLVRGYLESIPGYEERHRVLPGLAGLAQVAGDYYLTPHQKLRFDRLYIRHRSIG
                     FDLKLIILAFSIAFWFRWQDGWNGRLPRKWFRWASPYQPPA"
BASE COUNT          764 a          869 c          831 g          795 t
ORIGIN      
        1 ttactgcgcc aacgggttta tccaaagcgt tagaaatatc tgcatactcc caatcttcca
       61 ggaagcgtag cacgatcacc tggcgttggt ctggggtgag tttcgataac gccaagcgta
      121 catcccgtcg catcgactcc tccgcgaata catgtggagg ctgtttgtgc tcgtctatgc
      181 aacagtccgg atctagaggg agcgcacttt tgttctggtt acggtaataa tcgctaaccc
      241 agttatgggc catacggtaa agataggcgc gcagatattg gcgtggccca ccgccgcgct
      301 ttatggcaag cagaaatcga tggaaagttt cagcaacgca ttcctcagca agctctgaat
      361 cgcctagaag ccgcatcgta taccgaaata tttccgggct gtagcggtcg taaacctcag
      421 ccaggatatg ctcatcaaaa tcatggatct gcgcgcagag ctgctcttca gaaatcatta
      481 tcctttattt gtgacgcaaa actagtaaaa atgatacggc tcacaaggaa attcgattag
      541 aattggcgta ttgtttagag acaagaaaat cgccgcaagc aagcagtcaa cattcgctcg
      601 ctggatcagc tcgctttaca gcctactctc cggtcaaggg gcccgacagg gttatcttgc
      661 cgcagtagac cagggcgtaa tttccgcatc gaactttctg gcaacaatca tcctggctcg
      721 cagcatcacc ccaacggagc tgggcgtgta tggggttggc tttattgccc tgagattggt
      781 gcgatccctc caggaggggt tggtggtgca accaatgaat gtctatgggg cagggatgga
      841 tgagcattca tttcgccgct atgccaccag ctcgtctgtt ctgcaaatct cgctggcggc
      901 ctcctcatcg ctggtggcgg cgatctcggg ctggctgctg acagcgctgg gaaacgatac
      961 tgctggtccc gcactttttt cgttgtggtt cgcattcttg tggtggcaat tacaggaata
     1021 tatccgccgc atgctctaca cgcgtggcat ggtcttcagc gcagtggtca ataccatctt
     1081 tgccaacgcc tttcgtatct ttctgttgct atggtgggca aagcaaggca aattgacggg
     1141 tattcttggc ttggatgcca tcgcttgggg atcgctggta gccctgcttc ccgggctttg
     1201 ggcaacgcgc tcatattggg ttagagactt cccgaacttg aaacagacct ggctccgcaa
     1261 ttgggggtta ggtcgttggc tcttaggcag cagcctggcg aattgggtag ccgtggagtt
     1321 ctatccagtc ctgaccgctg gcatgatcag ttttgcagcg gctggcgcgt accgcgcctt
     1381 gcagaatctc gttgcgccta tccacctgct cttgcgagcc accgatacct tcctgacccc
     1441 acgcgcagcc cgcctatatg agctgaacgg acaaacggcg atgcggcgca cgctacgcct
     1501 gatttacctg atatctggga ttcccatatt gggcgtctta gccattgcta ttctcttccc
     1561 tacgccgttg ttacgactgc tctatggaga aacttatttg gcctatagca gcagcatggc
     1621 cttgatgggt ctcttctacg ctctgctgta tgcctactac cccctgcaga cagcattcaa
     1681 agcggcacgc ctaacccgcc ctatctttct tgccaacctg ggagccatcg cagccatgtt
     1741 cactatcggc atctgggcta tcctgcgctg gggagtggct gggaccatcg caggtcagac
     1801 tctgaatgcc ttaattgttt caattattct ttgggggacg tggattagga tactacgccg
     1861 gcagaactga aaatcagggg gatcgcgtca ctaacgttta acaaagccaa gggtttaggc
     1921 tgggggttgg tatggtgatg cccagcggaa ccatttcctg ggcaacctgc cattccaccc
     1981 gtcttgccag cggaaccaga aggcgatcga aaaggccaga atgatcaatt taagatcaaa
     2041 tccaatactt cggtgacgga tgtacaacct gtcgaaacgc aatttttgat gtggggtcag
     2101 gtaataatcg ccagcaacct gtgccaatcc agccaaacca ggcaagacac ggtgcctttc
     2161 ttcgtatcca ggtatcgact ccaggtagcc acgcaccagt accgttcgct gtggacgtgg
     2221 tccgacgaag ctcatctgtc ctagaaggat attgatcaat tgtggtagtt catccagtgc
     2281 ggtcttacgc aaaaaacggc ccactaccag aacacgctta tcactctcac ttgccagcac
     2341 cggaccagtg ctgtattcag cttccctgac cattgtcctg aacttgtatt ggtgaaaatt
     2401 aattccgcct tttcctactg agttctttac aaacagcagc ggtcccggtt cttccagcca
     2461 gatcacgagg atacccatta gccacactgg cagagataac accaagccta agagcgccag
     2521 gcttacatca aatgttcgtt tgagcagggg ttcggggtct gggtcgccga tcctattaag
     2581 gtgatgctgg tgcacttcca ttcggatgag ctgagagggg ggtgaatttt cctcccatag
     2641 actttcattc aagcctgtgg ctaaaagacc tcccgagaaa gaacccaaaa ctaccaaaga
     2701 caagtttata taatttacta cccaagccat gttcattggc tgcaagccag tgtaacctag
     2761 atatacccca atggagagca gaaaagccgc ccggtagcgc caatctcggg ggaaagaggt
     2821 gattgccaga gcacgctcca atattaaggt caatagggcg tgcaggataa agcgcccaat
     2881 cacattgcgc caggatgcct cattccagag cacttgagcg tagagccaag tagcgaacag
     2941 aagcacgaag gcggcaataa ataatgccga cgcgttggta atcgtttgcc gacctgtgtg
     3001 caacgaatgg ctttgagatt cctcgatttg cccttttccc atagtcttga caatggaaaa
     3061 ttatacctct gatataatcc tcgccagatc tgcgccgccc tcaacaaaaa actctcattg
     3121 ccctggctgc atccacaata atggtcgcgg tggcagctgg ggctctgatc tttcccaagg
     3181 ctatccagaa gtggagtgca cccctgggtc caagtctgga actgcccacc catacaccga
     3241 ccaaggagag cttggttag
//
LOCUS       DBNGSX010000219         2996 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_155615, whole genome shotgun sequence.
ACCESSION   DBNGSX010000219 DBNGSX010000000
VERSION     DBNGSX010000219.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_155615"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..899
                     /locus_tag="AC16CB_06620"
     CDS             114..899
                     /locus_tag="AC16CB_06620"
                     /inference="COORDINATES: protein motif:HMM:NF014571.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HIR3342177.1"
                     /translation="MQSVSKEQVIPALYAVGLEAGDGLLVHSAIQFLGRPKGGVDMYL
                     LALCEVINCDPEAASQPSASPQAGTLAVPAFNFAFARGEPYDPHSTSSQGMGSFSEYV
                     RQLPGAQRTPHPMQSLAIIGRYADELTGRDTPSAFDPGSAFERMFELDFKLLLLGADV
                     QAVSMVHYSEQRASVPYRYWKDFTGQVKTASGWQTRTYRMFARDLELDPQLNLHPVQE
                     LMEQRGQWRTMTVNYGQIASCRLVDFVAAADELLAGNPWALVG"
     gene            907..2196
                     /locus_tag="AC16CB_06625"
     CDS             907..2196
                     /locus_tag="AC16CB_06625"
                     /inference="COORDINATES: protein motif:HMM:NF027579.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HIR3342178.1"
                     /translation="MTKMLDLINELWFLKRNIISDDFDQALYRLAEEVPMTIHEVPTG
                     TPCWTWRVPEKWTCQEAYLETLDGRRLLDYADHPLHVVSYSLPFEGIVSHQELLSHLH
                     VHPRHPDAIPYVFKYYERDWGLCATQKLRDSLNDESYRVVIRTAFEAGSLKVGEVVIP
                     GQSEQTFLIVAHLCHPAMVNDDLSGVVVGVDIARRLLASPQPHYTYRLLILPETIGSV
                     AYLSHHEDLIPKMIGGLFLEMLGNDSPHALQGSFQPESQPDRCFNASLRDLDPQGYAG
                     AYRTIIDNDERQFNAPGVRVPMLSLSRVQAPKSSGLPPYPEYHSSLDTPAIVTQARLE
                     ASRDMVLGLIQAWERNQYVVNNFKGEIFLSGYGIWVDYRVNPEGHRRMFEIMERCDGS
                     RTVADIAHELSLPFKAVWEVVSLLEEKGLVWFSRTPA"
     gene            2259..>2996
                     /locus_tag="AC16CB_06630"
     CDS             2259..>2996
                     /locus_tag="AC16CB_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015016 - heparan sulfate
                     N-sulfotransferase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HIR3342179.1"
                     /translation="MNAYFSLKHYLKVARWKVRRGATASRWGLGELQRAPIVLGNAMP
                     KSGSHLIIQVLQGLTSLGPFVNPGFPPVNRGEDNRKLSDRAVLANIQRMQPGDIGYGY
                     ISATPTFIDALTRPRRATVFVYRDPRDMIVSHIFYATQMHPDHWMRRYYTETLSTMEE
                     RINAAIQGVDEPGSELTPLRRRYEGYLAWLEQPEVLSLRFEDLILNRQAVLKQLLDFI
                     RGRGLNLKMPRVQAVSILDASVAPKKSG"
BASE COUNT          603 a          926 c          835 g          632 t
ORIGIN      
        1 ttacgtatca accctatgac ccaacttggt atcttaactt tgtcgatctg tggtcttgac
       61 ccaatagggg tcgccttaat gagataggga agcaagcata gggaggtcgg ggtttgcagt
      121 cagtttcgaa ggagcaagtt atccctgcgc tttacgctgt tggcttggaa gccggcgatg
      181 ggctgctggt gcactctgcc atccagttcc tgggacgacc caagggcggg gtggacatgt
      241 acctgcttgc gctgtgcgaa gtgatcaact gcgacccaga ggcggcaagc cagccctccg
      301 cgtcacccca ggctggcacg ctcgccgtcc ccgcttttaa tttcgctttc gcccgcgggg
      361 aaccgtacga tccacacagc acctcttcgc agggcatggg gtcattctcc gaatacgtgc
      421 gccagctccc gggtgcgcaa cgcactccgc accccatgca atctctggca atcatcggtc
      481 gctatgccga cgaattgacc gggcgcgaca ccccctcggc tttcgaccca ggctcagcct
      541 ttgagcgcat gttcgagctg gatttcaagc ttctgctgct tggcgcggac gtccaggcag
      601 tctccatggt gcactatagc gaacagcgag ccagcgttcc ctatcgttac tggaaagact
      661 tcactggaca agtgaagacc gcctccggct ggcagaccag aacctatcgc atgttcgcgc
      721 gtgacctgga gcttgacccc cagttaaacc tgcacccggt acaggaactt atggagcaac
      781 gcggccagtg gcgtactatg actgtcaact atgggcagat cgcctcctgc cgattggtcg
      841 atttcgtcgc tgcagcggat gaactcctgg caggtaatcc gtgggctctt gtgggttgaa
      901 gatcacatga ccaagatgct cgacctgatc aacgaactct ggtttctcaa gcgcaacatc
      961 atctccgacg actttgacca ggcgctctac cgcctggcgg aagaagtgcc aatgaccatc
     1021 cacgaggtgc ctacgggaac accttgctgg acctggcgtg tgccggaaaa atggacctgc
     1081 caggaggctt atctggagac actggacggt cgccgcctgc tcgattatgc cgaccaccct
     1141 ctgcatgtgg tctcttactc gctgcctttt gagggcatcg tctcgcacca ggaactgctg
     1201 tcccacctgc acgtccaccc gcgccaccca gatgcgatcc cctacgtctt caagtattat
     1261 gagcgagatt gggggttatg cgctacacaa aaattgcggg actcgctgaa cgacgagtcc
     1321 tacagggtgg tgattcgcac ggcattcgag gcgggcagcc tgaaggttgg cgaggtcgtc
     1381 atcccaggtc aaagcgagca aaccttcttg atcgtcgccc acctgtgcca ccccgccatg
     1441 gtcaacgacg acctgagcgg cgtggtggtg ggggttgaca tcgcccgtcg cctattagcc
     1501 tccccccagc ctcattatac gtatcgcctg ttgatcctgc ctgagacgat cggctcggtg
     1561 gcctacctca gccatcacga agatctcatc cccaagatga tcggcggatt gttcctggag
     1621 atgcttggca acgactcgcc gcacgcactg caaggctcct tccaacccga gagccagcca
     1681 gaccgctgct tcaacgcctc tctgagggac ctcgaccctc agggttatgc gggagcttac
     1741 cgcaccatca tcgacaatga cgagcgccag ttcaacgctc cgggagtgcg cgtgcccatg
     1801 ctctcactct cgcgtgtgca agcgcccaaa tccagcggtt taccccctta tccggagtac
     1861 cattccagcc tggacacgcc tgccatcgtg acacaagcgc ggctggaggc gtcgcgtgac
     1921 atggtgctcg gcctgatcca agcctgggag cgcaaccagt acgtggtgaa caacttcaaa
     1981 ggcgagattt tcctgtcagg ttacggcatc tgggtagatt accgcgtcaa ccccgaggga
     2041 caccgccgga tgttcgagat catggagcgc tgtgacggct cgcgcaccgt cgctgatatc
     2101 gctcatgagc tttccttgcc cttcaaagcg gtttgggagg tggtctcctt gctggaagag
     2161 aaggggttgg tctggttcag ccgcacacca gcctgataca agacctgtga tccgtgactt
     2221 caaacatcat ccccaagatg taggtactca aagccgttgt gaacgcttat tttagcctga
     2281 aacattattt gaaggtcgcc cgttggaagg tgaggcgggg cgctacagcc tctcgctggg
     2341 ggctgggcga gctgcagcgc gctcccattg tgctgggcaa cgccatgcca aagtccggct
     2401 cgcacctgat catccaggtg ctgcaggggc tgacaagctt aggcccattc gtcaatccag
     2461 gcttcccgcc cgtcaatcgc ggcgaggata accgcaagct gagtgaccgt gcggtgcttg
     2521 cgaacattca gcgcatgcag cctggagaca tcggctacgg ctatatcagc gccacgccta
     2581 cctttatcga cgcactcacc cgacccaggc gcgctacagt ctttgtctac cgcgacccgc
     2641 gcgatatgat cgtctcgcac atcttctatg ccacgcagat gcacccagac cactggatgc
     2701 gccgctatta cacggaaacg ctgtccacga tggaggagcg catcaatgct gccatccagg
     2761 gcgtggacga acccggctcc gagctaaccc ccctccgccg gcgctacgag ggctacctgg
     2821 cttggcttga gcaacccgag gtgctcagcc tgcgctttga agacctgatc ttgaatcgtc
     2881 aggctgtatt gaaacaactg cttgatttca taagaggccg cggtcttaat ttgaaaatgc
     2941 cgcgcgttca ggcggtgagc attttggacg cctccgtcgc cccaaagaag tccggt
//
LOCUS       DBNGSX010000220         2206 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1540713, whole genome shotgun sequence.
ACCESSION   DBNGSX010000220 DBNGSX010000000
VERSION     DBNGSX010000220.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1540713"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1123)
                     /locus_tag="AC16CB_06635"
     CDS             complement(137..1123)
                     /locus_tag="AC16CB_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005964848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR3342180.1"
                     /translation="MSRIHTRTNQNGDINNKEGTKRLGLSILLNLAITLAEIIGGVYS
                     GSLSLLSDALHNFSDTASLGISLVAFKISTRGADRKNTFGYRRAQIIGALINLITLVL
                     IAVYLIKEALDRYFNPRPILGSVMLVVAIIGLLANLATAALLYRQSKNNLNIRSAFIH
                     IMGDAFSSVGVVVAGFLIIRYQLYLADTLLTLFISAYLLIHSTQLLRQTINILMQGVP
                     EGIDIDDIIASVKSVEDVLDVHHLHVWQLDETQSNLEAHIVIDQTRLENMTNIKRKIK
                     HHLASLFNITHATLEFEIEDCVPHDLVNCYETERSHYEIDRLIHQNIGKIGD"
     gene            1379..>2206
                     /locus_tag="AC16CB_06640"
     CDS             1379..>2206
                     /locus_tag="AC16CB_06640"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HIR3342181.1"
                     /translation="MRTVEIIAKKRDGGELSRNEINFFIQGYTRGDIPDYQAAALAMA
                     ILLRGMTPQETADLTMAMANSGEILDLAGVVPIAVDKHSTGGVGDKTTLVVEPLVAAC
                     GLAVGKMSGRGLSFSGGTLDKMESIPGYRTDLSTEEFIQQLKSVGLVLTGQTADLAPA
                     DGKLYALRDVTATVQSLPLIASSIMSKKIAAGAQAIVLDVKVGLGAFVQNLDEGRQLA
                     EAMVSIARFIGRRAVALLSDMNQPLGNAVGNALELKEAIQTLHGSGPADFLEHCRVIA
                     "
BASE COUNT          550 a          495 c          587 g          574 t
ORIGIN      
        1 cgtggatcat ttcctttgcg gctgagattt tccactgaat tatcgctcct cggttttgtg
       61 cataactcct aataccgcaa ttcctgcggc taaaccatac cacatttgta gtagattata
      121 ataaaaaatc tctaaattaa tcccctattt ttccaatgtt ttgatggatt aagcgatcta
      181 tctcgtaatg tgagcgctct gtttcgtaac agttgaccag atcatgaggt acgcaatcct
      241 caatttcgaa ctccagtgtg gcatgggtga tattaaataa gcttgccagg tgatgtttga
      301 ttttgcgttt gatattggtc atattttcca atctggtctg atcaatgacg atgtgcgctt
      361 ctaaatttga ttgcgtctcg tctaattgcc aaacatgcag atgatgaacg tctagtacgt
      421 cttcgacaga tttaaccgag gcaatgatat cgtcgatatc gatcccctct ggcaccccct
      481 gcatcaagat gttgattgtc tgcctgagca gctgagtact gtgaatgagc agataggcag
      541 agatgaacaa cgtcagcagt gtatctgcca aataaagttg gtagcggatg atcaaaaagc
      601 ctgcgaccac cacacccact gatgagaagg catcccccat aatatggata aaggcgctac
      661 ggatatttaa gttgttctta gattggcggt agagaagagc ggcggttgct agatttgcga
      721 gcaggccgat tatcgccaca accagcatga ctgacccgag aatagggcga gggttgaagt
      781 agcgatccag agcttctttt atcaagtaaa cagcgatcag caccaaggtg atcaggttga
      841 tcagcgcccc aatgatttgg gcgcgacggt agccaaaagt attcttgcga tctgctccgc
      901 gcgtagagat cttaaatgcg accaggctaa tacccagcga agctgtatcg ctaaaattgt
      961 gcaaagcgtc agagagaaga gaaagactgc ctgaatacac cccgccaatg atttccgcca
     1021 gggtgatggc taggttgagc aggatggata gccccagccg cttcgtaccc tccttgttat
     1081 ttatgtcgcc gttctggttg gttcgggtgt gaattcgtga catcaactct cctgtgaact
     1141 acatagatta taatgcattc gtggtcttaa ggaatttaca ggatattgac ataaattatc
     1201 tcccagcttc attatttggg tatcctgtct taagataagg gcattgggcg tacacagtcg
     1261 attgataaaa ggtggatatg ggtgggcgaa atccccaaaa cccaaaaacg cctcttttaa
     1321 tgtgtaattt ctgatttgtt cacaattgtt gtgaatgttc gttccgcagg agggtttcat
     1381 gcgcacagtg gagattattg ccaaaaagcg ggatggggga gaattgagcc gcaatgagat
     1441 taatttcttt atccaaggct acacgcgcgg cgatatcccc gattatcagg cggctgctct
     1501 ggctatggca attcttctca ggggcatgac cccacaggaa actgccgacc tgacaatggc
     1561 gatggccaat tctggcgaga tactcgacct ggccggggtt gtgcctatcg ctgtggataa
     1621 acactctacg gggggagtgg gtgataagac taccttggtg gtggagcccc tggtagcagc
     1681 ctgcggtctg gcggtaggca aaatgtcggg tcgcggcctg tcttttagcg gcggtacact
     1741 cgataaaatg gagtctatac cgggctaccg cacggacctg agcactgagg agtttatcca
     1801 gcagttgaag agcgttggtt tggtgctcac cggtcaaacc gcagacctgg ctcctgcgga
     1861 tggtaagctc tacgcgctgc gtgacgtgac cgctaccgtt cagtcattgc cacttattgc
     1921 atcatccata atgagcaaga aaatcgccgc gggagcgcag gctattgtat tggatgttaa
     1981 ggtggggctg ggagctttcg tgcaaaacct ggacgaaggg cggcaattgg ccgaggcgat
     2041 ggtatcaatc gcccgcttta ttgggcggag ggcggtggcg ctgctctcgg acatgaacca
     2101 gcctttggga aatgccgtgg ggaacgcatt ggaattgaaa gaagccatcc agaccttgca
     2161 tggtagcgga ccagccgact tccttgagca ctgcagggta attgct
//
LOCUS       DBNGSX010000221        12992 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_709558, whole genome shotgun sequence.
ACCESSION   DBNGSX010000221 DBNGSX010000000
VERSION     DBNGSX010000221.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12992
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_709558"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="AC16CB_06645"
     CDS             complement(<1..994)
                     /locus_tag="AC16CB_06645"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3342182.1"
                     /translation="MTKRLFTLISMLVLATMILAACAPQAAAPEATEAPPEAPAAFEC
                     TDAIGCVEIAPDEPVHIAFILTISGATAFLGQDSQGAVEIAIDDHGGKLLGHDIQLTG
                     EDGGCSAEGGQTAATKVASDPTILGVIGTNCSSAATAALPTISGAGLVMLSPSNTAPA
                     LTIEGQTWQPGYFRICHTDLFQGSVAAEFVYNELGATTAATIHDGSPYADQLQQVFSD
                     RFQQLGGTITFQGAVNVGDTDMRTILTTVAADSPDVLYYPIFEPEGPFITAQSSEIAG
                     LEDTILMGADGLLSDSFPEAAGANAVDMYMSGPYVSGAPYEEFLAKWEAKFGGVP"
     gene            complement(1231..1728)
                     /locus_tag="AC16CB_06650"
     CDS             complement(1231..1728)
                     /locus_tag="AC16CB_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR3342183.1"
                     /translation="MSIMTFELQQADIFYQLTSAQLEKISGIITEKIFNYGEMIFWEG
                     THSDELYVIAQGEVDILINPSLVSDQPEQEQEPATIATLRRGQSFGEIALIDRGIRSA
                     SARAAQANTRLLVIPSDRLLGICDTDPSLGYRLMRNLAADLALKIRNTDLSIRGQLLY
                     RKNFP"
     gene            1857..2876
                     /locus_tag="AC16CB_06655"
     CDS             1857..2876
                     /locus_tag="AC16CB_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342184.1"
                     /translation="MSTTDNQALPTQPSLMTSLMAGFDAVTNHLELILFPIILDLFLW
                     FGPHLRLKELVVEFTNQIGNLSGLDTPEVSNALQLNRELWLLVADRLNMFSALRAYPV
                     GIPSLMASRQPLEAPLVQPVGWEVSSVEGAAVWWFILSVMGLVVGALFFVLVAQASLH
                     GKIHWQIAIGQWPRSSLQVILLTLFIGILMLAISIPGSCLITALTLTGLPVSQISLLL
                     FGAVALWVLFPFLFSAHGIFTYQHKMWTSVRQGARVVRMTLPKTSLLFLSIVVISEGL
                     DTLWRVPAENSWMSLIGVTGHAFVSTSLLAASFIYYRDATRWVQRLIQQSLMVESKKL
                     RERIT"
     gene            3002..4921
                     /gene="gyrB"
                     /locus_tag="AC16CB_06660"
     CDS             3002..4921
                     /gene="gyrB"
                     /locus_tag="AC16CB_06660"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HIR3342185.1"
                     /translation="MVKGETFSYEAKDIQVLEGLEAVRRRPGMYVGGTDLKALHHLIY
                     EVVDNSIDEALAGACDRIEIIIHPDSSVTVSDNGRGIPVDIHPQIKKPALEVVMTMLH
                     AGGKFGGGSYKVSGGLHGVGVSAVNALSEWCQVEVRRDGKVHFQRYERGYPKNPVSVI
                     GKSDSKITGTTTTFKFDSEIFKGDVDYRFETLVQRFREMAFVTRGVTIFLMDERTDRQ
                     MTFYFEGGITSFVRYLNRNRSVLHPVVHLEREVDGITVDAAIQYTDAFSESVYAFANT
                     INTIDGGTHLTGLRAAITRTINDYARRNGLLKDSDPNFTGDDTREGLTAIVSIKHPDP
                     QFESQTKVKLMNPEVQTLSQQVVGEAFSVFLEENPSAGKSIVQKCLTSARARDAARKA
                     RDLVIRKSALESLTLPGKLADCSERDPQKTELYIVEGDSAGGSAKQGRDRHFQAILPL
                     RGKILNTERARLDKILANNEVKALISALGTSVGESFDVSGLRYGRVIIMTDADVDGSH
                     IRTLLLTFFFRYMLSLIEEGHLYIAQPPLYRIAYRNQIRYAYSDSEKDQVLESLGTSS
                     EKAVLSRYKGLGEMNPQQLWETTMDPAQRTLLQVTVEDAAEADRTFDMLMGSAVPPRR
                     RFIQTHARDVRNLDI"
     gene            4940..7225
                     /locus_tag="AC16CB_06665"
     CDS             4940..7225
                     /locus_tag="AC16CB_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR3342186.1"
                     /translation="MPVKKGPRPVAGPSLVKSMRKTVLPRPVTPLAKPGTNPRLAEAR
                     VLRREAVRQVANKVQPNRPRPVVRPASAPVRVMPRTPAPPPATKAVRRPAVPASGVRV
                     AAATAAAAAGFLALNAAAAHTDIASEIRSLQSSLSNLERRSTFSDIESDVVNIDSSLQ
                     RASELLESARGKGYAFQGDLETTLYNTMSTWESMRPQVNNLVRQQKNALKSQLVGVNP
                     MIQSLNRVVGNPTAATAQLRSTQNHVNGLLHNISRIENDIQNRYDAVETQAQKLNTRL
                     TQIHWAMDQLVQAKFQLDSGEDLVMAVPTRWDKEGKDDPEGVLYLSNRRLLFEQKEKI
                     ATKKVLFITTASELVQELLIDQPYSGISGVKAESKGLFGHQDFLMVSLSDPRLGDVSL
                     HINGQDSKDWAALIERARSGQIEAERANVSASFSVGDLTKPLTPADIMALQSEVNALQ
                     DEVMLKSARQELAELENDVRSLERKLAEVRARGYAVEKDLEADITILAAQWERVKGNA
                     EGTIEYQARLLGDQMGAIQDDLAKVVGMSANLTAARPMYMQVKSALASLEAQADAAET
                     TVLAQYDEYADEVESLQAHLEWISWMLEALATASFRLLATESGVAATEAVHLHPSREP
                     ENGVLFLTDQRLLWEDRVGEYELKVDIPTQLVQDVRKETVDGAEMEALVVRISSGDLA
                     PETRFQLTLPVADDWIKMVGRSRSGDYAQDRAVELSKDELERIRNAPQQCSNCGAALT
                     APILRGQTEIVCEYCGNVTRI"
     gene            7252..9444
                     /locus_tag="AC16CB_06670"
     CDS             7252..9444
                     /locus_tag="AC16CB_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3342187.1"
                     /translation="MVLERGTLVNNRYRVAEILGQGGMGSVYRAVDENLGVDVALKEN
                     LFTTDEYARQFRREAVILASLRHPNLTRVTDHFVIEDQGQYLVMDYIEGEDLRQRMDR
                     VGVLSEDEVVIIGAAICDALSYLHTRIPPIVHRDIKPGNVKITPQGQIFLVDFGLAKI
                     IQTGQSTTTGARAMTPGYSPPEQYGTARTDHRSDIFSLGATLYAALTGAIPEDALARA
                     MDQVKLTTIRRRNPKISRRMAGVIEKSLAVRPDDRYQDAEEFKQALLNVRGITTRRKT
                     GEYVVSPPPEFSEEMIKQGDSGVDVGGENYIPRAEIRSPLPVSSNVTLNESYTPAGRK
                     ARRSSVWTTLVLVVITLGMAGWAAGNFFPSWPSRLLSLINPPSDNPLVVVVGDPTSIQ
                     TNEISTSTHTPTSTSSPTLEPSPTTTPTSTLEPTPTDLPVQPSATITQMPTPVGGGNG
                     QIAYASDRSGKTQVWLVNVDGSSDPVQVTNMPEGACQPDWSPDGLRLVFISPCNQNED
                     LYPGAGLFVIHADGSGLTPLPNVPGGDFDPTWSPDGNLIAFTSLRNSGRPRIYVLDLR
                     DNSVTRLSGQYSYDRQPAWSPDGSRIAFVTTQKGPVQIWVMDSNGSNQAIYTRSADSI
                     NTHPVWSRDSDLILFNQVEPGGGPPYIVAVSYNDGQYNEFRYQLGPWPVREAQYSPDG
                     LWLVFESWPQGSNHDIYVMSASGAGRTQLTSWERFDFDPAWRPMVQAP"
     gene            9607..11019
                     /gene="gnd"
                     /locus_tag="AC16CB_06675"
     CDS             9607..11019
                     /gene="gnd"
                     /locus_tag="AC16CB_06675"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HIR3342188.1"
                     /translation="MGQNLVLNMNDHGYRIAVYNRTISKVDEFLETSAKGTEVVGAHS
                     LEELVSLLKRPRRVMLMIKAGQPVDDFIDLLIPLLEPGDIIIDGGNSNFQDSIRRTAY
                     LESKGLLFIGTGISGGEEGARYGPSIMPGGSTQAWPHVKDIFQSIAAKVEDGTPCCDW
                     VGENGAGHFVKMVHNGIEYGDMQLIAEAYHLMKSGLGLSYDELHQVFAEWNRGKLDSY
                     LIEISRDIMAFKDEDGTPLVEKILDTAGQKGTGRWTVVEALENGVPLTLVAEAVLARS
                     LSALKEERQVASKKLKGPRKTFSASRERAIDDIRHALYASKIISYTQGYMLMRTAAGE
                     YKWNLNYGGIALMWRGGCIIRSAFLGKIKEAYDRNPALPNLLLDPYFCREVSSSQAGW
                     RRVLANAVQAGIPLPAISSALAFYDGYRQERLPANLIQAQRDFFGAHTYERLDRPRGE
                     FFHTNWTGEGGAVTSGSYQA"
     gene            complement(11249..12727)
                     /locus_tag="AC16CB_06680"
     CDS             complement(11249..12727)
                     /locus_tag="AC16CB_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR3342189.1"
                     /translation="MLLLLGLVLIQSISLGRITNFPSRPANPVPVWGEKPVLGYFVFT
                     PSLASRLSREAGLSEAQFQAIEQITHQELEMLRAIEQESLPIIQDSKLSLQEKRSLIV
                     KMRYNERVRGVVRASKDKLKNTLGIFAYARLSQWIQRQWQNEREIHGSVETASGPRTY
                     RIFATRYDSSGAYTVALPDKCLKFANGGNHICDSDGYVAGAGYTVFLSYEGSTAAIVG
                     ESGPWNVDDNYWATTSDPTPRRMFADLALGMPEAQAAYFNGYNGGVDQFGRVVTAPFG
                     IDLARQVSIDIGLQPGNNDWIDVSFLWTEGWDGSADSQPPQSDATAIPQPTKETIIPI
                     EVASPNPDGSVVHVVQEGQTLWNIASAYQVSLQEILVLNGLSDGALIYPGQKLLIKLA
                     SQTPTLTSTSTPETEIPSSVSTATLRPTRTVVPRSATAIAQIVSTPLPPQNTPNAVPT
                     AGVSSSDPDAEANVDPFIIGIVALAGVGLFLLILGQWLNRRS"
BASE COUNT         3068 a         3171 c         3601 g         3152 t
ORIGIN      
        1 gtggaacccc accgaatttg gcttcccact tggccaggaa ctcttcgtag ggtgcgcccg
       61 aaacataggg gccggacatg tacatgtcaa ccgcgttggc gccagccgcc tcggggaatg
      121 agtcagacag caacccatcg gcacccataa ggatggtgtc ctccaagccg gcgatctcgg
      181 aagattgagc ggtgatgaaa ggtccttccg gctcgaagat ggggtagtag agcacatccg
      241 ggctatcggc agccacagtg gtcagaatgg tgcgcatatc ggtgtcgccc acgttgaccg
      301 cgccctggaa ggtgatcgtg ccgcccaatt gctggaaacg gtctgagaag acctgctgaa
      361 gctggtctgc gtatgggctg ccgtcatgga tggtcgcagc agtggttgcc ccgagctcat
      421 tgtagacgaa ctccgccgca acagaaccct ggaacaagtc ggtgtggcag atgcggaagt
      481 aacctggctg ccaggtctga ccttcgatgg tcagggcagg ggccgtgttg gatggggaca
      541 gcatcaccag gccagcaccg ctgatggtgg gcagggccgc ggtggctgcg ctggagcagt
      601 tggtaccgat cacacccagg atggtggggt cagaggcaac tttggtagct gcagtctgtc
      661 caccttcggc cgagcagccg ccatcctcac ccgttaactg gatgtcatgg ccgagcagtt
      721 tgccaccgtg atcgtcgatg gcgatctcga ccgcgccttg tgagtcttgg ccgaggaagg
      781 cggtggcgcc cgagatggtc agaatgaaag caatgtggac gggctcatcg ggtgcgatct
      841 ccacacagcc gatggcatcg gtgcactcga aagctgcggg agcttcaggc ggcgcctctg
      901 tagcctcggg ggcggcggcc tgtggggcac atgctgccag gatcatggtc gcaagtacca
      961 gcatcgatat cagggtaaac aatcttttgg tcatcttctt ctctccttaa tatattggga
     1021 tcattgtgtt gtgtgaggca ttactttatt ttttactgtt ttccgaaact acctcctctc
     1081 gatgatgatt ctcgttactc ttggatgtta tgcagtttag attgaagtga ggcaagtaag
     1141 gaactgcaaa gctatccttg ttgtgaagga cgcacatgac aacgatttta ccgataacat
     1201 ctcatatggt caaggactag ctcatgatat ttacggaaaa tttttacggt aaagtagctg
     1261 cccacgtatc gataaatctg tgttgcggat ttttaatgcc aggtctgcag ccaagttgcg
     1321 catcaagcga tatccgaggg atggatccgt atcacagatt cccagtaatc gatcgctagg
     1381 aattaccagc aagcgtgtgt tagcttgcgc ggcgcgggct gaagccgagc gtatgcctcg
     1441 atcgatcaac gcaatttcac cgaatgattg ccccctacgc agggtggcaa tcgtcgcggg
     1501 ttcctgttct tgctcaggtt gatcgctcac caaggaaggg ttgatcaaaa tatccacttc
     1561 tccctgggca atgacatata attcatcgct gtgggtccct tcccaaaaga tcatctcacc
     1621 gtagttaaat attttttcag tgatgatgcc cgagatcttc tccaattgag ccgaggtcaa
     1681 ttggtagaaa atatctgcct gttgtagctc aaaggtcatg atggacatac gatttttcta
     1741 gatctctggt gatattttat cgaggtgcta aagtctataa agtctagcat aaatcacaaa
     1801 aatgtcaatt ctagcgcggt gttctttaag accccctatg gtaaaatggg gtcgaaatga
     1861 gcacaactga taaccaggct ttgcctaccc aacccagcct gatgacctcc ctgatggctg
     1921 gttttgacgc ggtcacaaat caccttgaat tgattctgtt cccgatcatt ttggatttat
     1981 tcctttggtt cggaccccac ttacgcctga aggagctagt cgtagagttc acaaaccaga
     2041 tagggaattt atccgggctc gatacacctg aggtatctaa tgcattgcag ctaaaccgcg
     2101 agctgtggct attggttgct gataggctca acatgttttc cgcactacgg gcttatccag
     2161 ttggaatacc cagcctgatg gcttctcgtc aaccgctgga agcgccactc gttcaaccag
     2221 tggggtggga agtgtcttcg gttgagggtg cggcagtctg gtggtttatt ctcagtgtaa
     2281 tgggtttggt ggttggtgct ttattctttg ttttggtggc gcaggcttct cttcacggca
     2341 agatacactg gcagatagcg attggtcaat ggcctcggtc ctctttgcaa gttattttac
     2401 taaccctgtt cattgggata ttgatgctag cgattagcat cccagggagc tgtttgatta
     2461 ccgcgttgac attgacgggc ttaccggttt cccaaatcag cctgttgctt tttggtgctg
     2521 tggcactttg ggtgctattt cccttcttgt tttcggctca cggcatcttc acctaccaac
     2581 acaaaatgtg gacatcggtc cggcagggag ccagagtagt tcgtatgacg cttcccaaaa
     2641 ccagccttct tttccttagt attgtggtga tcagcgaagg actggatacg ctctggcggg
     2701 tacctgcaga aaactcgtgg atgtcgttga tcggggttac cgggcatgcg tttgtgtcta
     2761 cgagcttgct ggcagcaagc tttatttact atcgcgacgc tacacgatgg gtacaaagat
     2821 taattcagca gtcattaatg gttgaaagca agaaattgcg tgagcgcatc acgtgactct
     2881 aggattaaga caagaaaatc gaaaaataaa atttatgggc gtatcaatac gtctcaatat
     2941 tcgcccattt tttttcctga ttctctaatc ttacaactct gatatcgctt gaggagatga
     3001 cgtggtaaaa ggtgaaactt tctcgtatga ggcaaaagat atacaagtgc tggaggggct
     3061 ggaagccgtg cggcgccgac ctgggatgta cgtgggcggc accgacctca aggctttaca
     3121 tcacctaata tatgaggtag tggataactc catcgatgaa gctctggcag gggcgtgtga
     3181 ccgtatagaa atcatcattc atcccgatag cagcgttacg gtcagcgata atgggcgcgg
     3241 catcccggta gatatccacc cccaaattaa gaagccagcc cttgaagtag tgatgactat
     3301 gctacatgcc ggcggcaagt ttggcggagg tagctataaa gtctccggtg gtctgcatgg
     3361 ggtgggcgta tcggcggtta atgccttatc ggaatggtgc caggtagaag tccgccggga
     3421 tggcaaagtg catttccagc gttacgagcg cggttatccg aaaaacccag tgagcgtgat
     3481 cggcaagtcc gactcaaaaa tcacaggtac gactaccacg tttaaatttg atagtgagat
     3541 tttcaaaggc gatgtggatt accgcttcga gacgctggtg cagcgcttcc gagagatggc
     3601 ttttgtcacg cgcggtgtga ccatcttctt gatggatgaa cgcacagacc ggcagatgac
     3661 cttctacttc gaaggcggta tcacatcctt tgtgagatat ctgaatcgca atcggagcgt
     3721 cttgcatccg gtggttcatc tggagaggga ggtggatggt atcaccgtcg acgctgccat
     3781 tcagtacacc gacgcctttt ctgagtctgt atatgcgttt gctaatacga tcaataccat
     3841 cgacggtggc actcacctga caggtttacg tgcagccatt acccgcacga tcaatgacta
     3901 tgcccgccgg aacggcttgc ttaaggattc tgaccccaat ttcaccggcg acgacacgcg
     3961 tgaaggtttg actgcgatcg tcagcatcaa gcaccctgac ccgcagttcg aaagccagac
     4021 caaagttaaa ttgatgaacc cggaggtgca aaccctctcg caacaggtgg tgggtgaggc
     4081 tttcagcgta ttcttggaag aaaacccgtc tgcgggcaaa tcgatcgtgc aaaaatgcct
     4141 gacctcggcg cgggcgcgag atgcagcccg taaagcgcgc gacctggtaa tccgaaagtc
     4201 tgcgctggag agccttacat tgcccggcaa gctggctgat tgttcggagc gcgacccaca
     4261 gaagactgag ctgtatatcg tggagggaga ttcggctggc gggtcagcga agcaaggacg
     4321 tgaccggcac ttccaggcga ttttgccgct gcggggaaaa atcctcaata cggaacgggc
     4381 gcgattggat aaaatcctgg cgaataacga ggtcaaggca cttatctcag ccctggggac
     4441 tagtgttggt gagagctttg acgtttcggg tttgcgttat gggcgggtga tcatcatgac
     4501 cgatgctgac gttgatggta gccatattcg caccctatta ttaaccttct tttttcgata
     4561 tatgctatcg ctcatcgaag agggtcattt gtacattgca caaccaccgt tgtaccgtat
     4621 tgcttataga aaccagatcc gttacgccta tagcgatagc gaaaaagatc aggtgcttga
     4681 atctttaggt actagctcag aaaaagccgt tctttctcgt tataaaggtt taggcgagat
     4741 gaatccccaa cagctttggg agacaaccat ggacccggcc cagcgaacgc tcttgcaagt
     4801 tacagtggaa gacgctgcag aagctgatcg cacttttgat atgctcatgg gttcagcggt
     4861 cccaccacgc cgtcgtttca tccaaaccca tgcccgagac gtgcgaaacc tggacatata
     4921 aagtgcgata ggaggaatta tgccggttaa aaaaggcccg cgccctgtgg ctgggccgtc
     4981 attagttaag tctatgcgta aaacagtatt gcctcgcccc gtgacaccac tggctaagcc
     5041 ggggacaaat cctcgcttgg cggaagccag ggtgttgcgc cgcgaggcag tccggcaggt
     5101 tgcgaacaag gttcagccta accgcccaag accggttgtg cgtccggctt ctgcaccggt
     5161 gcgagtaatg cccagaactc cagccccccc tccagccaca aaggcggtgc gcagaccagc
     5221 agtgcctgcc agcggtgtac gggtcgcggc agcgactgct gccgcagcag cagggttttt
     5281 ggctttaaac gccgcagccg cgcacacaga cattgcatct gagattcgat cgctgcagtc
     5341 gtccctgtcc aatttggaga gacgcagcac ctttagtgat atcgaatcag acgttgtcaa
     5401 tatcgattca tccttacagc gcgcttcgga gttgttagaa agtgcccgag gtaaaggcta
     5461 tgcctttcaa ggtgaccttg agaccacgtt gtataacacc atgagtactt gggaaagtat
     5521 gcgccctcag gtcaacaacc ttgttcggca acagaaaaat gctctgaaaa gccagctcgt
     5581 tggtgtcaat ccgatgatcc agagcttgaa ccgggtggtt ggcaacccaa ctgcagcgac
     5641 ggcgcagctg cgcagcactc aaaaccacgt caatggccta ttacataata tctcacggat
     5701 agaaaacgat atccagaacc gttatgatgc ggtggaaaca caggcacaaa agctgaacac
     5761 gcgcttgacc caaatccatt gggcaatgga tcagctggtt caggccaaat ttcaacttga
     5821 cagtggagaa gacctggtga tggcggtgcc cacccgctgg gacaaagagg gcaaggatga
     5881 cccggaaggg gttctctacc tgagtaaccg ccgtcttttg tttgagcaga aggaaaaaat
     5941 cgccacgaag aaggtcttgt tcattacaac cgcgtccgag ctggtacaag aattgctcat
     6001 tgatcaaccc tatagcggca tcagtggcgt gaaggctgaa tctaagggat tatttgggca
     6061 ccaggatttt ctgatggttt ctttgagtga cccaaggctt ggagatgtct cgttgcatat
     6121 aaacgggcaa gactccaaag attgggctgc gcttattgag cgcgcccgtt cgggacaaat
     6181 cgaagccgag cgcgctaacg tatcggccag cttttcggta ggagatctga ctaaaccact
     6241 cactccagcc gatattatgg cgctacagag cgaagtcaat gccttgcagg atgaagtgat
     6301 gctgaaatcc gcaaggcagg aattggcgga gttggagaat gacgtgcgct ccctggagcg
     6361 aaagctggcc gaagtgcgcg cccgcggcta tgctgtcgag aaagatttgg aggccgatat
     6421 caccatcctc gctgcacagt gggagcgggt caagggcaac gctgagggca caattgaata
     6481 tcaggctcga ttgcttggcg accagatggg tgccatccag gatgatctag cgaaagtggt
     6541 cggcatgtct gccaacctga ctgcagcccg cccaatgtat atgcaggtta agtcggcttt
     6601 ggcctcttta gaggcacagg ctgatgctgc tgaaaccacc gttctggcac agtatgacga
     6661 gtatgctgat gaggtagaga gccttcaggc tcacctggaa tggattagct ggatgctgga
     6721 ggcgctggct acggcttctt tccgattact ggctactgag agcggtgtag cggcaacaga
     6781 ggcagttcat ctccatccat cccgtgagcc ggagaatggc gtgcttttcc tgacagacca
     6841 gcgccttctg tgggaggatc gtgtgggcga atatgagtta aaggtcgata tcccaacgca
     6901 attggtccag gatgttcgca aagagacggt cgatggcgca gagatggaag ccctggttgt
     6961 gcgcatcagt agcggtgact tggctccgga aactcgtttt cagctcactc taccagtggc
     7021 agatgactgg ataaaaatgg ttggcagatc gcgcagcggc gattatgccc aagaccgggc
     7081 agttgaatta tccaaagatg aattagaacg aatacgaaat gcccctcagc aatgtagtaa
     7141 ttgtggtgcg gctttgaccg caccgatttt gcgaggccag actgagatcg tttgtgagta
     7201 ttgtggtaat gtcacgcgaa tttaggtcgg ttttactgga tatcagctag aatggttctt
     7261 gaacgcggca ccctcgtcaa taatcgctac cgtgtggccg aaatccttgg tcaaggtgga
     7321 atgggctcag tttatcgcgc cgttgacgag aacctggggg tggatgtagc tctcaaggag
     7381 aatctattca caactgatga gtatgctcgc caattccgtc gagaagctgt catactcgcc
     7441 tcgttacgcc atccaaatct gacacgagtt acggaccact tcgtcatcga agaccagggt
     7501 cagtacctgg taatggatta tattgagggc gaagacctgc gccagcgtat ggatcgggtg
     7561 ggtgtgttaa gcgaggatga ggtggtcatc atcggagcgg ccatctgtga tgcgcttagc
     7621 taccttcaca cgcgtattcc gcccattgtc catcgggata tcaagccagg taatgtcaag
     7681 atcacgccac aagggcagat cttcctggtg gacttcggtc ttgcgaaaat cattcagacg
     7741 ggacagtcga caacaacggg tgcgcgagct atgaccccgg gctactcgcc acctgagcaa
     7801 tacggcaccg cccgcactga tcaccgttcc gacatcttct cgctgggagc aaccctttac
     7861 gctgccctga ccggcgcaat tcctgaagac gcgctggcga gggctatgga tcaagttaag
     7921 ctaaccacca ttcgccgccg caatccaaaa atttctcgtc gcatggctgg ggttattgaa
     7981 aaatctttgg cagttcgccc ggatgatcgt tatcaggatg ccgaggagtt caaacaggct
     8041 ttgctcaacg tacgtggtat caccacgcgt cgcaagacgg gcgaatatgt tgtgtcccct
     8101 ccaccagaat tctcggaaga gatgattaaa caaggggatt caggagtgga tgttgggggg
     8161 gagaactata ttccgcgagc agagattcgc tcaccgttgc cggtttccag caatgttact
     8221 ttgaatgaat catacacgcc ggctggtcgc aaggcgcggc gttcatccgt ttggacgaca
     8281 ttggttttgg ttgttatcac gcttggtatg gcgggttggg ctgcggggaa ttttttccct
     8341 tcatggccat cacgcttgct ctccctaatt aatcctccta gcgataatcc gttggtcgtt
     8401 gtcgtgggtg atcctaccag catacagact aatgagatat cgacttctac tcatactcct
     8461 acaagcacat cttcacccac attagagcct agccccacga ccacgccaac atcgacactg
     8521 gaacctacac ccactgattt gccagtccaa ccgtctgcaa caataactca gatgcctacc
     8581 ccggttggcg gtggcaatgg ccagattgcg tatgcttcag atcgttcggg taaaacgcag
     8641 gtttggcttg tcaatgtaga tggcagtagt gatccagtgc aagttacgaa tatgcccgaa
     8701 ggggcttgcc agcctgactg gtcgcctgat ggattacgcc tggtatttat ttcgccatgc
     8761 aaccagaatg aggatttgta cccaggcgcg ggcttgtttg tcatccatgc agacggctct
     8821 ggcttgaccc ccttgcctaa cgtgcccgga ggggacttcg atccgacctg gtcaccggat
     8881 ggcaatttga ttgctttcac ctcgttgcgc aacagcgggc gcccgcgcat ctatgtatta
     8941 gacctgcggg ataattcagt cacgcgcctt tccgggcagt attcctatga tcggcaacct
     9001 gcctggtcgc ctgatggcag caggattgca tttgtgacca cccagaaagg tccggtgcag
     9061 atctgggtta tggattccaa tggcagcaac caagctattt acacgcgcag cgctgattcc
     9121 atcaacaccc atccggtatg gtcaagggac agcgatctta tcctgtttaa tcaggttgag
     9181 cctggagggg gaccaccgta tatcgtggcg gtatcctata atgatgggca gtacaatgaa
     9241 ttccggtatc agctgggacc ttggcctgta cgcgaggctc aatactcacc ggatggactg
     9301 tggctcgtat ttgaaagctg gccgcagggt agcaaccatg atatctatgt tatgtctgca
     9361 agtggggctg gcaggacgca attgactagc tgggaacgct tcgactttga tccagcctgg
     9421 cgtccaatgg tgcaagcacc ctgacgagaa ataatgtaaa tttctcaaaa tacccctcgc
     9481 gacccctttt gaggtgagta ttctcaaaac tggcggtaca tacttttcca caatgtaaaa
     9541 aggaatttcc tagtgaaaag caacgcagaa cctgaatgcg atcttggttt gatcggtttg
     9601 gcggtcatgg gccagaatct ggtattaaat atgaatgatc atggctaccg catcgcggtt
     9661 tataaccgca cgatatctaa ggtagatgaa tttctcgaaa ccagtgcaaa aggaaccgaa
     9721 gtggtagggg ctcattcttt ggaggagctt gtgtcgctgc tcaagcgtcc ccggcgggta
     9781 atgctcatga tcaaggctgg acaaccagtg gacgatttta ttgatttgct gatcccattg
     9841 ctagagccgg gcgatattat catcgatggt ggtaactcca attttcagga ttcgatccgc
     9901 agaacagcct accttgagtc aaagggtttg ctttttatcg gtacgggcat ctctggggga
     9961 gaggagggcg ctcgctatgg cccgtctatc atgccaggag ggtctaccca agcctggcca
    10021 cacgttaagg acattttcca aagcatcgcc gctaaagttg aggatggcac accttgctgt
    10081 gattgggtag gtgaaaacgg ggcgggtcac tttgtcaaaa tggtgcacaa tggtatcgag
    10141 tatggcgata tgcaattgat tgcagaggcg taccacttga tgaagtcagg gttgggttta
    10201 agctatgatg aactgcacca agtatttgct gagtggaatc gaggaaaatt agactcctac
    10261 ctgatcgaaa tctcacgcga tatcatggca ttcaaagacg aggatgggac tcctctagtt
    10321 gaaaaaatcc tggatacggc cggtcagaag ggaactggtc gttggacagt ggttgaggct
    10381 ttggagaatg gcgtacctct gaccttggtt gcggaagcag tgctggcacg ttcattgtcc
    10441 gcgctcaagg aagagaggca agtcgcctcc aagaagctca agggaccgag aaaaaccttc
    10501 tcagcctcga gagagagagc cattgacgat atccgccatg cattatatgc atcgaagatc
    10561 atctcctaca cgcagggata tatgcttatg cgcacggccg ccggcgagta taaatggaac
    10621 ctcaactatg gagggattgc cttgatgtgg cgcggcgggt gtattattcg ctccgccttc
    10681 ctggggaaaa taaaagaagc ttatgatcgt aatcctgcct tacccaacct gttgttggac
    10741 ccatatttct gccgcgaagt gtcgtcttcc caggctggtt ggcgcagggt gctagcgaac
    10801 gcagtacagg cggggatccc actcccagca atttccagcg cactggcgtt ttatgatgga
    10861 taccgtcagg aacgcttacc agcgaattta atccaggctc agcgggactt cttcggggcg
    10921 catacctacg agcgcctcga tcggccgcgg ggcgaattct tccataccaa ctggaccggc
    10981 gagggtgggg cagtcacttc ggggagctat caggcctaaa tagcagtatc cattgcatcg
    11041 ggacctggcc cgagttgaac agaataatcc aagttgccat aatagccatg cccatcatag
    11101 tcttttatag ggaggaatat tgtatgtcgg cgggtcacgg attacagcga tatcccccca
    11161 attccccgta ttgcggtaat gcatacacct ggcagggcaa ttactttgca ctaccaaaat
    11221 gatggtcaca tggtaatttg aaaaattatt atgatctgcg gttcaaccac tggcctaaga
    11281 taagcagaaa taaacctacg cctgcaagtg ctacaatccc gatgatgaat gggtccacat
    11341 ttgcttcagc gtctgggtca gaagaggaga ctcctgccgt ggggactgcg tttggggtgt
    11401 tctggggtgg caaaggtgta gagacgattt gggcgatggc ggtagcagac ctagggacca
    11461 ctgtccgagt cggtcgcagg gtggcggtcg agacactgga tggtatttcc gtttcgggtg
    11521 tactggtgga agtcaaggtg ggtgtttggc tagcgagttt gatcagcagt ttttgaccag
    11581 gatagattag tgccccgtcg cttaatccgt tcaacactag gatctcttga agactgactt
    11641 gatacgctga ggcgatattc caaagcgttt gtccctcttg caccacgtgg accactgagc
    11701 catctgggtt gggtgacgcg acctcgatgg gaataatggt ttcttttgtg ggctgcggga
    11761 ttgcggttgc atcggattgg ggaggttgtg agtccgcgct gccatcccag ccctcggtcc
    11821 acaagaatga aacatcaatc cagtcgttgt tgcctggctg gagaccgata tcgatactca
    11881 cctggcgtgc caggtcgatg ccaaagggtg cggttaccac ccgcccaaat tgatccacac
    11941 caccgttata cccattaaaa taagctgctt gtgcttctgg catgcccaat gctaggtcgg
    12001 cgaacattcg ccggggcgtt gggtctgagg tggttgccca gtaattatca tccacgttcc
    12061 aagggccaga ttctccaacg attgctgccg tactgccctc atagctgaga aatactgtat
    12121 agccggcacc ggcaacatag ccatcgctgt cgcagatgtg gtttccgccg ttggcgaact
    12181 ttaggcactt atccggaagg gcgacagtat atgctccgct tgaatcataa cgggtcgcaa
    12241 aaattcggta ggtgcgcggt cccgaggccg tctccacaga gccgtgaatc tccctctcat
    12301 tctgccattg gcgctggatc cattgagaga gccgtgcgta agcgaatatc cccaacgtgt
    12361 ttttaagttt atccttggat gccctgacaa ctccccggac gcgttcgtta tatctcatct
    12421 tgacgatgag cgaacgtttc tcttgcaggc ttagcttgga atcctggatt ataggtaggc
    12481 tctcctgttc gatggcgcgc agcatctcta gttcttggtg tgtgatttgc tcaatggctt
    12541 ggaattgagc ctcggatagc cctgcttccc tggataatcg gcttgccagg ctgggtgtga
    12601 agacgaagta accaaggaca ggcttttcac cccaaacggg tacgggatta gccgggcgag
    12661 atgggaaatt tgtgatacgc ccaagggaga tcgactggat gagcactagc cccaatagca
    12721 aaagcaaagc taggctgatt agctgatgat aaagcctgaa tctcaggtca agggatgtaa
    12781 aagcccatcg acttttttgc gttgctctca taggtttgag cgaattataa tggtcttttt
    12841 agtgatgagc ttggagaagc cacgcgtaac caagagggga tatgcaagca tcaagtgttg
    12901 catatcccca ccttaggaag ttaacctaaa actatgggca ggaccgggtc ggtaagattc
    12961 tatggagtga tcgcaaatac gcgggccggg aa
//
LOCUS       DBNGSX010000222         2300 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1402403, whole genome shotgun sequence.
ACCESSION   DBNGSX010000222 DBNGSX010000000
VERSION     DBNGSX010000222.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1402403"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="AC16CB_06685"
     CDS             <1..1177
                     /locus_tag="AC16CB_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024634.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HIR3342190.1"
                     /translation="QLGGKLRYLYTSAPGGGDPQVLLQETIRAVKTEPRIRVFTETIL
                     TELRGYTGQYTSTVQQSDGSTLELQHGAVVVATGGQPYKPREYLYGEHPRILTQLELE
                     DLLTHPANHMPQVIQSGQSFVMIQCVGSRDDEHPYCSRVCCTQALKNALAIKGKFPDA
                     RVTVLYRDIRTYGFREQLYRQARQAGVVFLEYSEVDKPTVTPDGERLQIEVAIQPENE
                     AVNLAADWLVLSAGIEPEPDNPSLAQILKVPLNEAGYFLEAHVKLRPVDFAAEGIFLA
                     GLAHSPRQIEETIAQAHAAAVRAVGLLSRSRLEATPIIASVNPKLCAACGLCVEICPY
                     GARLLEPGMSHAEVIDVLCQGCGACVVACPNKASQQKGFEFAQVFGMVEEALNLPHS"
     gene            1193..1621
                     /locus_tag="AC16CB_06690"
     CDS             1193..1621
                     /locus_tag="AC16CB_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HIR3342191.1"
                     /translation="MNNDKFEPHIVAFLCNWCTYTGADLAGTSRLQYPSNIRIVRTMC
                     SGAVDPVYVLKLLMDGADGVLVGGCHPGDCHYQSGNYKARRRYVALREILKAAGFDEQ
                     RVWLRWISASEGQKFADTVTEMVSALTELGPNPTREIWAV"
     gene            1674..>2300
                     /locus_tag="AC16CB_06695"
     CDS             1674..>2300
                     /locus_tag="AC16CB_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342192.1"
                     /translation="MKTIIAVENSGVLAGVRHFLKRLMETGVVEAIYVPLKVDGGTII
                     PALVTDPRWLDQADPISPVMPINGARAVSALTGKHPPGRLGAVLRSCEIRALLELVKL
                     QQASLDGLSLISVDCPGTCELRDYEAIKRNGGFNLESFLESASAGSEPQGFNLRAACQ
                     MCLQPDYEAADAHLQLFGADVQQGIPVVLRDDIAMNLELVAEPNPSDGR"
BASE COUNT          500 a          617 c          671 g          512 t
ORIGIN      
        1 gcagttagga ggaaaactgc gctacttgta caccagtgcg cccggtggag gggatccgca
       61 ggttctcctt caggagacga tccgggcggt gaagacagag ccacgaatcc gtgtattcac
      121 cgagacaata ttaaccgaac tgcgcggtta taccggtcaa tataccagca cagtgcagca
      181 atcggatggt tcaacccttg agctgcagca tggtgcggtg gtagtggcta cgggcggtca
      241 accgtacaag ccgcgtgagt atctctacgg cgagcaccca cgcattttga cccaattgga
      301 gctggaagac ctgctaactc atccagcgaa tcacatgcct caagtcatcc aatcgggaca
      361 atcgtttgtc atgattcagt gcgtaggctc gcgcgatgat gagcatcctt attgctcacg
      421 cgtctgttgc actcaagccc tcaagaatgc cctagcgatt aaaggtaaat tccctgatgc
      481 gcgcgtgacg gtgctctatc gcgacattcg cacttatggc ttccgcgagc aattgtaccg
      541 gcaggcgcgt caggctggcg tggtctttct ggagtacagc gaggtggata aacccaccgt
      601 cactccagat ggtgagagat tacaaatcga ggtcgcaatc cagccggaaa atgaagcggt
      661 caacctggct gccgactggc tggtgctcag cgcggggatt gaacccgagc cggataaccc
      721 ctccctggcc caaattttga aagtgccgct aaacgaagcg ggatacttcc tggaggctca
      781 cgtcaagttg cgcccggtag attttgctgc tgagggaatc ttcctggctg gcctggctca
      841 ctcgccgcgt cagatcgagg agactatcgc ccaagctcat gctgctgcgg tgcgagcggt
      901 aggtctgctc tcccgttcgc ggctggaggc cacaccaatt attgccagcg ttaaccccaa
      961 attgtgcgcc gcctgcgggt tgtgtgtgga gatatgccct tacggtgcgc gccttttgga
     1021 gcccggcatg agccatgccg aagtcatcga tgttttatgc cagggctgtg gcgcctgtgt
     1081 cgtcgcctgc cccaataaag ccagccaaca gaaaggcttt gagtttgccc aggtgtttgg
     1141 catggtagaa gaagcgctca accttcccca ttcctaatta atacgaggca ttatgaacaa
     1201 cgacaaattt gaaccgcaca ttgtagcatt cctgtgcaac tggtgtacct ataccggcgc
     1261 agacctggcg ggtacttcgc gcttgcagta cccgtccaac atccgcatag tccgcacgat
     1321 gtgcagcggg gcggtcgatc ccgtctatgt gctgaagctc ttgatggatg gtgcagatgg
     1381 cgtgctggtc ggtggctgcc atcctggcga ttgccactac caatcgggca attacaaagc
     1441 gcggcgccgc tacgttgctt tgcgggagat cttgaaagct gctggattcg atgagcaacg
     1501 tgtctggctg cggtggatca gcgccagtga aggacagaaa tttgctgata cggtgacgga
     1561 gatggtatct gctctcactg agctgggccc aaaccctact cgggaaatat gggcagttta
     1621 ggcattaaac ggcggtactg attacgctct gcgtcctaac gagcgaggta tatatgaaaa
     1681 caatcatcgc agtagaaaat agcggggtgt tagctggggt taggcacttc ctgaagcgtt
     1741 tgatggagac tggcgtggtg gaagcgatct atgttcctct aaaagtcgat ggcgggacca
     1801 tcatacctgc ccttgtaact gatccgcgct ggctagacca ggccgaccca atatcacccg
     1861 tcatgcccat caacggagcg cgcgccgttt ctgcgctaac cggcaagcac cctcccgggc
     1921 gcctgggagc tgtcctgcgg tcatgcgaaa tccgtgccct gctggaattg gtaaagctgc
     1981 agcaagcttc actggatggc ctgagcctga tctcagtgga ttgtcctggc acctgtgagt
     2041 tgagggatta tgaggccatc aagcgtaatg gtggctttaa cctggagagt ttcctggaaa
     2101 gtgcgtctgc cggatcggaa ccacaaggtt ttaacctgcg tgccgcctgc cagatgtgcc
     2161 tccagccaga ctacgaggcg gcggatgctc acttgcaact ctttggcgcg gatgtgcaac
     2221 agggcatacc ggtggtgctg cgggatgata tcgcaatgaa ccttgagcta gttgccgaac
     2281 caaacccttc ggatggacgc
//
LOCUS       DBNGSX010000223         4099 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1748686, whole genome shotgun sequence.
ACCESSION   DBNGSX010000223 DBNGSX010000000
VERSION     DBNGSX010000223.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1748686"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="AC16CB_06700"
     CDS             complement(<1..1091)
                     /locus_tag="AC16CB_06700"
                     /inference="COORDINATES: protein motif:HMM:NF013381.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR3342193.1"
                     /translation="MMKHRERVQMALNHETPDRCPLQVSFTPEFADRLRADLQIKGQT
                     IHNPHGGGNTYELERALGEDMLLVSVGWANSYYQQSGTYTDEWGITWKPVEYTTPYGV
                     GHYTEPFGHPLTDDELVYGYTGPDPSRKELYNDAARVIREFKDEFWIVGVTVTTIFEC
                     AWALRGYERLLSDFILNPDLAEHILEIPYRYHLTAAKKLVEMGVDMIWIGDDVGAQFR
                     MLISPQHWRQYLKPRMANFIAELKSINPQVKVAYHSDGFVYPIIPELIEIGIDVLNPI
                     QPASMDPERVKKEFGDRLCFWGSIDEQHTLPFGTPADVRAEVHTRLKTIGKNGGLIIG
                     PTHHVQMDTPLENFWAMVNTVTQTRYADL"
     gene            complement(1091..2245)
                     /locus_tag="AC16CB_06705"
     CDS             complement(1091..2245)
                     /locus_tag="AC16CB_06705"
                     /inference="COORDINATES: protein motif:HMM:NF013381.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR3342194.1"
                     /translation="MNPRERVMLALEHKEPDRIPIDLGATIVSSITKNSYVSLKNHLG
                     VSIDEINILDYVQQLPYLDEALLQRFDVDFRMLQLPPAVATGPDIFEEGDYYAFIDRW
                     GSKLHMPKEGGYYFDWVDFPIKKFTLEALDQYTWPRPPSDEYALLREQAKYLYENTDY
                     ALVGSALIGGGIFEQPARTIGMENFLMAIAAEPKFADRLMERITDIYIESCNNYLDEV
                     GQYIQVFAYWDDVNTQNGWMINPDTYRKMIKPKQRRLVDAIKKKTDAKLFFHGCGATF
                     DLIPDLIDLGFDILNPVQVSARGMDTKRLKQMYGKEITFWGGGVDTQRVLPFGTPAEV
                     IDEAKRRIDDLAPGGGFVFAAVHNIQPLVPPENIIALFDTASEYGKYRND"
     gene            complement(2269..3288)
                     /locus_tag="AC16CB_06710"
     CDS             complement(2269..3288)
                     /locus_tag="AC16CB_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3342195.1"
                     /translation="MSSQTKGVNSIASQPYKETFHLGKFARANGTQLGILGVFFTLWF
                     IFVVSAPDTFLAPQIYNAFMSTVPFFAIMAIPLTMVVIMREIDLSFPSIMAMGMVAFI
                     LTYNATTSVALAFVTALSIGILIGWINGIIIVKFGIPSLVATIGTQFFWRGAVLVLSQ
                     GVSGSLIYVKESLLHPVLVGKIGGAFPVQMLWLIAIAILGWVLLNRHKFGAHIYLIGD
                     NVKSAELMGINTGRTRTLAFILLGLVSAFAGVVTSLHVAFFWPSLGEGYLLRTLASVF
                     LGGTSVFGGTGTILGTFIGAFIIGAIEAATVAVGLTGFWTQLIYGLIIVLSVTMHAYL
                     RRRVE"
     gene            complement(3307..4065)
                     /locus_tag="AC16CB_06715"
     CDS             complement(3307..4065)
                     /locus_tag="AC16CB_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3342196.1"
                     /translation="MEQYRLELRNVSKNFGEVQALENIDFQLGQNEVVGLLGDNGAGK
                     STLIKIVTGYYQPSSGEILFNGQKVDHLSVSKARQLGVETVYQERALAELQTLWRNIF
                     LGRELVNKWGLLDVPRMRQETHRLMTQSMGFTSLAVSPESKVGTFSGGEKQGVAIVRA
                     LYFDAEIIILDEPTMGLSLKETAKLVNFVRDIKMAGKSAIFIDHNILHVYNVADRVVV
                     IDRGRIAGQFLTKDINLDTLMERMIRVAETGSLD"
BASE COUNT         1045 a         1053 c          999 g         1002 t
ORIGIN      
        1 caaagatcag cgtatcttgt ctgtgtgacg gtattgacca tcgcccagaa gttttccaag
       61 ggggtatcca tctgcacatg atgggttggc ccaataatca acccaccgtt cttgccaatc
      121 gtttttaaac gagtgtgcac ttcagccctg acatccgccg gtgtaccaaa tggcaatgtg
      181 tgctgctcat caatggagcc ccaaaaacac aacctatcac caaattcctt tttcacccgt
      241 tccggatcca tactggcggg ttgaattgga ttaagaacgt cgatgccgat ctcgatcagc
      301 tcagggatga taggatagac aaagccatcg gaatgataag ccactttaac ttgcgggttg
      361 atcgacttca attcggcgat gaagtttgcc atgcgtggct ttaggtattg acgccaatgc
      421 tggggagaga tcaacatacg gaattgcgcg cccacgtcat cgccaatcca aatcatgtcc
      481 acgcccatct caaccagctt tttggcagcc gtaagatggt atcgataagg gatctccaaa
      541 atatgctcag caagatctgg attgagaata aaatccgaaa gcaggcgttc atagcctcgt
      601 aacgcccaag cgcattcaaa gatcgtggtc accgtaacgc caacgatcca aaattcgtct
      661 ttgaactctc ggataacgcg agctgcatcg ttgtagagtt ccttgcgact ggggtcgggg
      721 cctgtataac cataaaccag ctcgtcatcc gtgagtgggt gcccaaaggg ttcagtgtaa
      781 tgaccaactc cataaggtgt cgtgtattcg accggtttcc aggtaattcc ccactcgtcg
      841 gtataggttc cactctgctg gtagtaggaa tttgcccagc caaccgaaac aagcagcata
      901 tcctccccga gcgcccgttc caattcgtac gtattaccgc cgccatgcgg attgtgaatc
      961 gtctgacctt ttatttgtaa atctgccctc aggcggtcgg caaactcagg cgtaaaacta
     1021 acttgcagcg gacagcggtc cggggtctca tgatttagag ccatctgtac gcgttctctg
     1081 tgcttcatca ttaatcgttc ctatactttc catattccga agctgtgtcg aacagcgcaa
     1141 tgatgttttc cggcgggact agtggctgaa tgttgtgaac agcagcaaag acgaacccgc
     1201 ctccgggtgc caagtcatcg atgcgacgct tggcctcgtc gatcacctcc gccggggtcc
     1261 cgaatggcaa gactcgctga gtgtcgacgc cgccacccca aaatgttatt tctttgccat
     1321 acatttgttt tagtcgtttg gtatccatcc cgcgcgctga cacctgtacc gggttgagaa
     1381 tatcaaatcc gaggtcaatt aaatctggga tcagatcgaa tgtggctccg cagccatgaa
     1441 agaagagttt agcatccgtt ttctttttta tggcatctac cagcctgcgt tgtttgggct
     1501 tgatcatctt acggtaggtg tccgggttga tcatccaacc attctgggta tttacatcgt
     1561 cccaatacgc gaagacttgg atgtactgcc cgacctcatc taaataattg ttgcaagact
     1621 cgatatatat gtcggtgatc cgctccatca ggcggtcggc gaatttaggt tctgccgcta
     1681 tcgccatcaa gaagttctcc attccaatcg tgcgagctgg ctgctcgaaa atccccccgc
     1741 caattagtgc actcccgacc aaggcgtaat ccgtgttctc ataaagatat tttgcctgct
     1801 cgcgtagaag agcatactca tcggacggag gacgcggcca tgtatattgg tcgagcgcct
     1861 caagcgtaaa ttttttgatc gggaagtcaa cccaatcgaa gtaatatccg ccttctttgg
     1921 gcatgtgcag tttagagccc caccgatcga tgaatgcgta atagtccccc tcttcgaaga
     1981 tatccggccc tgttgccaca gcaggcggta attgcagcat gcggaaatcc acatcgaagc
     2041 gctgaagaag agcttcgtct aaatagggaa gctgttggac gtaatcgaga atattgatct
     2101 cgtctataga gacgcccaaa tggtttttca gtgagacgta cgaatttttt gtgatcgatg
     2161 agacaatagt cgcccccagg tcaattggaa tccggtctgg ctccttgtgc tccagagcca
     2221 gcatgactcg ctcacgtggg ttcatgcata acccctactc cagccagatc attccacacg
     2281 tctgcgcagg taagcgtgca ttgtcaccga aagtacgatg atcaaaccgt aaatcaactg
     2341 ggtccaaaag ccagtcagac caacagccac ggtagccgct tcgatggcac cgatgatgaa
     2401 agcgccgata aaggttccca ggatggtgcc ggtgccgcca aatacagatg tgccgcccaa
     2461 gaaaacagac gccagggtgc gcagcagata accctcacct aagctgggcc agaagaaggc
     2521 aacgtgtaag ctggtgacca ccccagcaaa ggcagatact aggccaagca gtataaaagc
     2581 caacgtgcgg gtgcgaccgg tgttgatacc catcaattca gcgcttttga cgttatcacc
     2641 aatcaaatat atatgggcgc cgaatttgtg gcggttgagc agaacccagc cgaggatggc
     2701 aatcgcgatc agccagagca tttgcacggg aaacgcacca ccgatctttc cgaccagcac
     2761 ggggtgcaga agcgattctt taacataaat caggctgcca ctcaccccct gtgataaaac
     2821 aagcactgcc ccacgccaga agaattgtgt gccgatggtg gcaaccaggg acgggatgcc
     2881 aaattttacg atgattatgc cgttgatcca accgatgagg atgccgatgc tcaaggcagt
     2941 cacaaatgcc agagccacgc tggttgtggc attgtaggtt aagatgaaag ccaccatgcc
     3001 cattgccatg atggatggga aagagagatc gatttcacgc ataataacca ccatggtcag
     3061 cggtatagcc atgatggcaa agaagggaac ggtagacatg aaagcgttgt aaatctgggg
     3121 cgccaggaaa gtatcggggg cggatactac aaatatgaac cacaaggtaa agaaaacccc
     3181 tagaataccg agctgggtac cattggcgcg ggcgaatttg cctagatgga aggtttcttt
     3241 atagggttgg ctggctatgg agttgacacc ttttgtctgt gagctcatcg catccttcct
     3301 tatgcattaa tcaagactgc cagtttctgc aactcgaatc attctttcca tcaatgtgtc
     3361 caaattgatg tccttggtca agaactgacc tgcgatacgg ccgcgatcga tgaccaccac
     3421 ccggtcggcg acattgtaaa catgcagaat attatgatca atgaatatcg ctgatttacc
     3481 cgccatttta atgtcacgca cgaaattgac gagtttcgcg gtttccttga gcgataggcc
     3541 catggttggc tcgtcaagga tgatgatttc ggcgtcgaaa tagagggcac gcacgattgc
     3601 gacaccttgt ttttcaccac ccgagaaggt accaaccttc gattcggggc taacagctaa
     3661 ggaggtgaag cccatggatt gcgtcatcaa tcggtgggtc tcctgcctca tcctggggac
     3721 atcgagcaag ccccatttat tgacgagttc gcgccccagg aatatgttac gccacaaggt
     3781 ttgcaattcc gccagggcgc gttcttgata cacagtttct acccccaatt gtcgggcttt
     3841 tgagacggag aggtgatcta ctttttggcc attaaacaaa atctctcctg agctaggttg
     3901 ataatagcca gtgacgatct tgattagagt ggattttccc gctccattat cacccaggag
     3961 gcctactact tcattctggc ctaattgaaa atcgatattc tcaagggcct gcacttcccc
     4021 aaagtttttg gatacattcc tgagctctag gcgatattgc tccatattcc tctccaaaag
     4081 tgctcaatca cgaatacat
//
LOCUS       DBNGSX010000224        11588 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_848774, whole genome shotgun sequence.
ACCESSION   DBNGSX010000224 DBNGSX010000000
VERSION     DBNGSX010000224.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11588)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11588)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11588
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_848774"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="AC16CB_06720"
     CDS             <1..706
                     /locus_tag="AC16CB_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR3342197.1"
                     /translation="TWAKVIPGVYTASYFANTDRSNAQIASFYPGAMAYAAQLSLHEL
                     SNNPPDLVIISPNDPGAMNCYVDECLEMGIPYLYDPSQQIVRMSKDELSRGIQGAMAV
                     FVNDYEFALIQKMTGKTPSDIMERAKFLVVTRGELGATVYSGDEEYHIPVVPPSQISD
                     PTGVGDAFRGGFLTAYSHSLDYLTCGQMGSLAATYCLEQHGPQGHSYTPAEFVARFRQ
                     HFDDQGKLDVLLGDEG"
     gene            765..2024
                     /gene="ahcY"
                     /locus_tag="AC16CB_06725"
     CDS             765..2024
                     /gene="ahcY"
                     /locus_tag="AC16CB_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HIR3342198.1"
                     /translation="MDNYDIKDTKLAEGGRRKIDWAAREMPVLRLIRERFEKERPLAG
                     MRIAACLHVTTETANLMQALHAGGADIVLTASNPLSTQDDVAASLVTHFEIPVYAIKG
                     EDNVTYYRHINAALDHKPHMTMDDGADLVGTLHKDRRELLGNMIGGTEETTTGVIRLR
                     AMAADGALVFPVIAVNDAMTKHFFDNRYGTGQSTIDGIIRATNTLLAGKAFVVAGYGW
                     CGRGLAMRARGMGANVIITEVDPLPALEAVMDGFRVIPMSEAAEIGDIFCTVTGDINV
                     IDSQHFEVMKDGAIVANSGHFNVEINIPALESMAVDKRLVRPFVEQYALSDGRRINLL
                     GEGRLINLAAAEGHPPSVMDMSFANQALAAEYMVKNVDKLEKKVYAVPEEIDKEIARI
                     KLESMGVKIDVLTAQQVKYLNSWEEGT"
     gene            complement(2111..2446)
                     /locus_tag="AC16CB_06730"
     CDS             complement(2111..2446)
                     /locus_tag="AC16CB_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HIR3342199.1"
                     /translation="MAYDKNLAARMRQILDELNPPSLTEKHMFGGVGYLVQGNMACGV
                     HKDELIIRVGPEAYIDSLNLPGARPFDLTGRPMNGWVSVRSAGFESDQSLREWIEQGL
                     AFAKTLPAK"
     gene            complement(2511..2981)
                     /locus_tag="AC16CB_06735"
     CDS             complement(2511..2981)
                     /locus_tag="AC16CB_06735"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3342200.1"
                     /translation="MKTDIIIDYLADHPDLLAVLASWFYAEWGHSKPDFTIQFFEDEL
                     RGRMNRDQLPLTLVAFLNDNPVATVSLLLKEMETHPQFLHWLGSVYTLPDYRHHGIGS
                     QLLEHAASEAKRLGVRELYLWTRHVESLYARFGWVIIQRPVYRGRPVAIMRRSL"
     gene            complement(3038..3517)
                     /locus_tag="AC16CB_06740"
     CDS             complement(3038..3517)
                     /locus_tag="AC16CB_06740"
                     /inference="COORDINATES: protein motif:HMM:NF027484.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HIR3342201.1"
                     /translation="MSRDKQEAVYLPPTRAIAAFPPIIQATATPIPEQAPTQAVTSTI
                     NPPCTDNLLYIEDQTIPDGSLVSAGEVLDKRWFVENNGSCNWGKDYRLKLISGFDLGA
                     LPEQGLYPARSGTQAAIRIVFTAPAEPGVYRSAWQASNPAGQPFGDPIYIEVQVASD"
     gene            complement(3633..4715)
                     /gene="recA"
                     /locus_tag="AC16CB_06745"
     CDS             complement(3633..4715)
                     /gene="recA"
                     /locus_tag="AC16CB_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR3342202.1"
                     /translation="MNRKNTSSSRNQDDSRSIALEKALGDITKRYGDGTIMRLGEAHK
                     LMVEAIPTGSLSLDIALGVGGIPRGRITEVYGPESSGKTTICQHIVAEAQKLGGTCAF
                     IDMEHALDPTYAEHCGVDVDSLLISQPDMGEQALEITETLVRSGAIDVIVIDSVAALV
                     PRAEIEGDMGDPQMGMQARLMSQALRKLSGAIKQTNTAVVFTNQLRQKIGVMFGNPET
                     TTGGMALKFYASVRLDVRRIQSIKLGAEIIGNRTRVRVVKNKVAPPFRTAEFDIMYNE
                     GISKVGDLIDLATELEIITKRGSFYSYGDIRLGQGRENAKDFLRQNPEMADEIELAIR
                     QQSLSGVMPLPFGGEGDEGGLDDDEA"
     gene            complement(4814..5845)
                     /locus_tag="AC16CB_06750"
     CDS             complement(4814..5845)
                     /locus_tag="AC16CB_06750"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR3342203.1"
                     /translation="MNSIPSPTASPPTPTPLTETEDLPETNLGLLALTMPTPAPDPLR
                     FSFPTPGSPPVSAWRPPLYPVPWVPTPFDHFYFVRPIAADEVNWPLADYRYGGVFLPE
                     VVHTGIDIPAPSGTPVLAAGPGRVTWAGYGLYRGVEDTTDPYGLAVAIKHNFGYNGET
                     LYTVYGHLQRIDVLVGQQMNTGDAIGLVGDTGKVTGPHLHVEVRLGRNNFFESRNPEL
                     WLAPPEGWGILAARLTNTRGERLYSHLISVHSKSGTRFWAVKSYGQGAAISDDYYQEN
                     LVLGDLPAGDYVVWIPFEGSTYNLDVTISPGMVTFFRFRGRNGFDSGLPPTPKPDFTP
                     PAAQISPTP"
     gene            complement(5989..8106)
                     /locus_tag="AC16CB_06755"
     CDS             complement(5989..8106)
                     /locus_tag="AC16CB_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR3342204.1"
                     /translation="MKNANDTSPSEPTGDPITRQIRRVLMVLLVIITVVVVGFFLDGR
                     INGRGPLAQVQSTSNQTPSETANTALPASETASQTAISPSLTPTISPPEISDTPVPGA
                     TSITSQSGASSASATDLIPNSGTLFLSIDEGGYKHLFAYHPQNLPFTRMTSGTWDDID
                     PSISPDGSRLAFASSREGFWDIYILDISTGKTTRFTDTPEFDGAPAWSPDGQWLVYES
                     YTDTLSTLPPQGATLSEATTTQITATPPVTVTVPNLEILIRPADGSQPAIRLTNDPAA
                     DYAPVWSPAGRQIAYVSNRTGENEVWLADLDRIDDRFHNISNNPKANDAYPAWSPDGT
                     RLSWTRVIDSYQNIVSLDINQPGAEPHLLGGGNRPAWSPDGDIVLTTLPTPNRTYLTA
                     YRADASGLALPPLPLPGQPAGLTWGRVSLPQPLPSFLSQVARITTTPAADAQLTPATN
                     IPAGRQRVVELEDVQAPYAKLHDSVDESFNALRLEVASRLGWDFLATLENAYVPLTSP
                     LFPGLLNDWLYTGRAIVINPAPINAGWMVIIRDDFGPTTYWRVYLRTRFQDGSQGMPL
                     HDIPWDLNARYAGDPRDYEQGGSLAPAIPPGYWLDFTNLAASYGWERLPALSTWRSAL
                     PAARYNEFVHTDDLDWFSAMVEIYPVEAVYTPTPIQPPTFTPTPTRRPTRTPTPTRTP
                     FPSRTPTITRTPMPLQQTISPTP"
     gene            complement(8385..9602)
                     /locus_tag="AC16CB_06760"
     CDS             complement(8385..9602)
                     /locus_tag="AC16CB_06760"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR3342205.1"
                     /translation="MYPLEFDNLTSIVARVLQIEDITRGDPKKDFIARYRGQLYSEDT
                     AAAYDQLASALRPLEVTPYFRKEQESHVVILALGVNRPKPSKSWVNLVLFLLTVISVF
                     LAGTYYTLLGTYDGPMNPTFRSLLPYVLQSTGGGAAFAASLLAILLAHEFGHYLAGRY
                     HRTHVTLPYFIPFPFSAFGTLGAFIQLKEPPKNRRILLDIGIAGPLAGLVVAIPVLIL
                     GLSLSQVNPIPAQLPPGQGLQIEGNSVIYLLTKYLVFGKLLPEPGNYGNLPPWMYWVR
                     YFFTGRPLPLGGLDVLLHPVAWAGWAGLLVTALNLIPAGQLDGGHVLYVLLGKKSSFL
                     RPIILGALVLLGLVWPGWWLWAFLIFMLGRFHAEPLDQITSLDPPRQAIAILGLLIFF
                     LVFTPVPLLTVGL"
     gene            complement(9664..9760)
                     /locus_tag="AC16CB_06765"
     tRNA            complement(9664..9760)
                     /locus_tag="AC16CB_06765"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9724..9726),aa:Sec,seq:tca)
     gene            9846..10961
                     /locus_tag="AC16CB_06770"
     CDS             9846..10961
                     /locus_tag="AC16CB_06770"
                     /inference="COORDINATES: protein motif:HMM:NF021539.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HIR3342206.1"
                     /translation="MYDSSSTSDVHPENDSQDKQTQSSQESDAVWTFRGYRLKSSEFT
                     TAMVHFFRAEISRANVWRTRLDATTNWAVVTTGATITIAFNPSGYHGVIILNTLLITL
                     FLFIEARRYRYYELWSYRVRLMETDFFASMLVPPFHPAPDWAEALAENLLHPDYPISM
                     WEAFGRRFRRNYMWIYIILAVAWVAKLWLLTPGPMTSWADFLAAASIGSLSGLIVLLI
                     GVVFNAALFLIGVLTTTLHKATGEVLPRYIQDIKILGPATATGQDTESRRAWFRQSRR
                     RQQLLAFVVTDQAQIVSQEILKEMARGVTSLEGVGMYTGKRHSVLMVALTTTEVAQLK
                     ALVSKTDANAFVIVSPAQEVLGRGFIPLKTKETEREN"
     gene            11015..>11588
                     /locus_tag="AC16CB_06775"
     CDS             11015..>11588
                     /locus_tag="AC16CB_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342207.1"
                     /translation="MNETHQIVRRLSRVQWIILSLGILITSGVELFQYMRGGDLVEVL
                     VEWLIEVFFLTLLLVVAIRLYAKVNQQLKRSQAVHPRQGSLIQLSTKLAASHDKEEIC
                     HILVKELQELIDHAKIEIFLIDHASGNRRRYGITNEGHSEEPVDSARPTRLEIPLRAG
                     QERLGGLLVEVEQARGVSEDEHAILVSAANL"
BASE COUNT         2644 a         2912 c         3349 g         2683 t
ORIGIN      
        1 aacctgggct aaggtcattc caggggtgta caccgcatct tattttgcca ataccgatcg
       61 ctctaacgcc cagattgcca gtttctatcc gggcgcaatg gcatacgcag cgcagctatc
      121 tttgcacgag ctcagcaaca atccacctga cctggtgatt atctcgccta acgatcctgg
      181 tgccatgaat tgttatgtag atgagtgcct ggagatgggt attccctacc tgtatgatcc
      241 aagccagcag attgtgcgta tgtcgaaaga cgaactcagc cggggcatcc agggcgcaat
      301 ggcggtcttc gtaaatgatt atgagttcgc tttgattcaa aagatgaccg ggaaaacccc
      361 ctcggatatc atggaacgcg ccaaatttct ggtggtaacg cgtggcgagc taggcgcaac
      421 cgtctacagc ggggatgagg agtaccatat tccagtggtt ccaccttcgc aaatttctga
      481 tcccaccggt gtgggggatg ccttccgagg tggcttcctg actgcttata gccatagtct
      541 ggattatctc acttgcgggc aaatgggatc attagcggca acatattgtt tagagcaaca
      601 cggccctcaa gggcattcct acacgccagc cgagtttgtt gcccgcttcc gccagcattt
      661 tgacgatcag ggtaagctgg atgttctgtt gggggatgag ggatagggta tataattcat
      721 cctggccctc actgaatggg aattttttct tacaggagaa acgcatggat aattatgata
      781 ttaaagatac caaacttgcg gagggaggcc gtcgcaagat agattgggct gccagagaaa
      841 tgcctgtgtt aaggctgatt cgcgagcgtt ttgaaaaaga acgcccgctg gcggggatgc
      901 gcattgccgc atgcctgcac gtgacaaccg aaacagccaa cctgatgcaa gccctgcacg
      961 cgggtggtgc cgacattgtc ttgactgctt ccaacccgct gtccacccaa gacgatgtgg
     1021 ctgcctccct ggtgacacat ttcgaaatcc cggtatacgc cattaaaggg gaagataatg
     1081 tcacctacta ccggcatatc aacgctgctc tggaccataa acctcatatg accatggacg
     1141 atggcgctga cctggtgggg actctacata aagatcgccg cgagttactt gggaatatga
     1201 tcggcggcac cgaagagacc acgactgggg tgatccgtct gcgtgcaatg gctgcagatg
     1261 gtgccctggt tttcccggtc atcgcggtca acgacgccat gaccaagcat ttcttcgata
     1321 accgctatgg aaccgggcaa tcgacgattg atggcatcat ccgcgccacc aatacgctgt
     1381 tggctggcaa agcatttgtt gtggcaggct atggctggtg cgggcgcggt ctggctatgc
     1441 gtgctcgcgg catgggcgcc aatgtaatca ttacggaagt agatccgcta cctgcactag
     1501 aggccgtcat ggatggtttc cgggtcatcc ctatgagtga agcagccgaa attggggaca
     1561 tcttctgcac cgtcactgga gacatcaacg tcatcgacag ccagcacttc gaagtgatga
     1621 aggatggagc cattgtggct aactcgggcc attttaatgt cgagataaat atccccgccc
     1681 tggaatcgat ggcagtggac aagcgcctgg tgcgaccctt cgtcgagcag tatgcactgt
     1741 ccgatgggcg gcgtatcaac ctgttggggg aagggcgcct gatcaacttg gcagccgctg
     1801 agggtcaccc gcccagcgtg atggacatgt cctttgccaa tcaggcactc gcggcagagt
     1861 acatggtgaa aaatgtggac aaacttgaga aaaaggttta tgccgtgccg gaagagattg
     1921 ataaagaaat cgctcgcatc aagctggaat cgatgggcgt gaaaatcgac gtgctgactg
     1981 cgcaacaggt gaagtactta aattcttggg aagaaggaac gtaactatca agtgcctgaa
     2041 atcgcaggca gcgcgatatc gaacgcaggt ctttcccttg tatccccgtc cggtaatggg
     2101 cggggatttt ttatttggct ggcagggtct tggcaaaagc caggccctgc tcaatccact
     2161 ctcgcagcga ttggtcggat tcgaaacccg ctgagcgaac gctaacccag ccgttcatcg
     2221 gcctgccagt caggtcgaag gggcgggcgc cggggagatt aagggagtct atataggctt
     2281 ctggcccgac gcggatgatc aattcatcct tgtgcacgcc acaagccatg ttcccttgca
     2341 ccaggtagcc tactccaccg aacatgtgct tctcggtcag gcttggggga tttaattcgt
     2401 caagtatctg gcgcatgcgt gccgccagat ttttatcgta agccactcag accttctcca
     2461 ttctccttat atcgtgtaac ttctgccaga aactaacagg cttttggttt ttataaactt
     2521 cttcgcatga tggcgaccgg gcgtccccga tagacaggtc gttggatgat gacccagcca
     2581 aagcgagcgt agaggctctc aacatggcgg gtccagagat atagctcgcg tacgcccagg
     2641 cgcttcgcct cgcttgctgc atgctcgagc aattgcgagc caatgccatg atggcggtaa
     2701 tctggtaagg tgtaaacgct tcccagccaa tgtaaaaatt gcgggtgagt ctccatttcc
     2761 tttaacaata agctgacggt ggcaaccgga ttatcgttca gaaaagctac cagagtcagt
     2821 ggtagctggt ctcgattcat acgacctctc aattcatcct cgaaaaattg gatggtgaag
     2881 tcgggcttac tatgacccca ttcagcatag aaccaggatg caagaacagc cagcaggtct
     2941 ggatggtctg ccagataatc aataatgata tcggttttca tgatgggagc ctgctctcta
     3001 tgactgataa ttcacttctc agcctttccc cattccctca atccgaagca acctgtactt
     3061 cgatatatat tgggtcgcca aacggctggc cagcggggtt tgacgcttgc caagcgctgc
     3121 ggtatacccc tggttctgca ggggcagtaa acacgatgcg aatagcagcc tgcgtgccgc
     3181 tacgtgctgg atagagtccc tgctcgggta gcgcacccag gtcaaaaccg gaaatcagct
     3241 tgaggcggta gtcttttccc cagttgcagg agccattgtt ttctacgaac cagcgtttgt
     3301 caagcacctc gcccgcagag accagagagc cgtctgggat agtctgatct tcaatataaa
     3361 gcaggttgtc agtgcagggt gggtttatgg tagaagtgac tgcctgggtg ggagcttgct
     3421 ctggaattgg agttgccgtc gcttggatga tcggcgggaa agccgcaatt gcgcgagtag
     3481 gaggcaggta gacagcttcc tgcttatctc gcgacaaagc actgcatccc gcagctatcc
     3541 ccatcagaca aagatagatt agcaggatgc gtaagcgtaa aattccgatc atcttcgatc
     3601 aggcaagcta tccggagatc gcaaggttcg gtctatgcct cgtcatcgtc caacccgccc
     3661 tcgtcaccct ctcctccgaa aggcagtggc attactccgc tcaaagactg ctggcggatt
     3721 gccagctcga tctcgtcagc catttcgggg ttctgtcgca ggaaatcctt ggcattttcg
     3781 cggccttgac ccaggcggat atctccgtat gaatagaaag atccccgctt ggtgataatc
     3841 tccaactcgg tagccagatc aatcaggtct ccgaccttgg atattccttc gttgtacatg
     3901 atgtcgaatt cggcggttcg gaagggcggg gcgactttgt tcttcaccac gcgcacgcgc
     3961 gtgcgattac caataatctc tgcccccagc ttgatggatt ggatgcggcg cacgtccagc
     4021 cggacagaag cgtagaactt gagcgccatg ccgccggtgg tggtctcggg gttgccaaac
     4081 atcaccccga tcttctggcg cagctggttc gtgaatacca ctgcggtgtt ggtctgcttg
     4141 atcgcgccgg acaattttcg caatgcttgc gacatcaatc gagcttgcat gcccatttgg
     4201 gggtcgccca tgtcgccctc gatttcagcg cgcggcacca gtgcagctac cgagtcgatc
     4261 acgatgacat cgatcgcccc cgagcgcacc agggtttcag tgatttccag ggcttgctcg
     4321 cccatgtcgg gctgtgaaat cagcaagcta tccacatcga ccccgcagtg ctctgcatag
     4381 gtggggtcaa gggcatgctc catgtcgatg aaggcgcaag ttccgcccag cttctgtgct
     4441 tcggcaacga tatgttgaca gatggtggtt ttacccgagg actctggtcc atatacttcg
     4501 gtaatacgcc cgcgcgggat gccgcctacg cctaaggcaa tgtcgagaga aagggagccg
     4561 gtggggatgg cttctaccat aagcttgtgc gcctcgccca agcgcatgat cgtaccatcg
     4621 ccgtagcgtt tggtgatgtc gccgagagcc ttctcgaggg cgatgctgcg tgaatcgtct
     4681 tgattgcgag aggatgaagt gtttttacgg ttcatggatc aaaaactcct gctggctgaa
     4741 attctaaaga tcaaacttat agataagttt gccgcagtgg catgcgcata gtttagcaca
     4801 aatgttcgac ctgtcaaggg gtaggagaaa tttgcgctgc cggaggggta aaatccggct
     4861 tcggcgtagg tggtagcccg ctgtcgaagc catttctccc gcgaaatcga aagaacgtga
     4921 ccatcccagg cgagatggtc acatcaagat tataggtgct gccttcaaag gggatccaaa
     4981 ccacataatc cccagcaggc aggtcgccta atactaaatt ctcctggtaa tagtcgtcgc
     5041 tgatcgctgc cccttgtcca taagacttga ccgcccaaaa acgcgtgccc gacttggagt
     5101 gaacgctgat caaatggctg tagagccgtt caccacgggt atttgtcagg cgtgctgcca
     5161 ggatgcccca gccctcaggc ggggctagcc atagttctgg attgcggctt tcgaagaagt
     5221 tgtttctacc tagtcgcacc tcaacgtgca ggtgcgggcc ggtgactttt ccggtatcgc
     5281 ccaccagccc aatggcgtcg ccggtgttca tttgctgccc taccagcacg tcaatgcgtt
     5341 gcaggtggcc gtagacagta tacagcgtct cgccgttgta accaaagttg tgtttgatag
     5401 cgactgccag cccataggga tcggtcgtgt cttcaacgcc tcgatagaga ccgtagcctg
     5461 cccaggtgac ccttccaggg ccggctgcca gcactggggt gccgctgggc gcgggtatat
     5521 cgatgccagt atgcacgacc tcgggcagga atacaccccc ataacggtaa tcagccagag
     5581 gccagttcac ctcgtcggcg gcaatggggc gcacaaagta gaaatggtca aaaggggttg
     5641 gcacccatgg aacggggtaa agcggtggtc tccatgctga gactggtggg gatccgggcg
     5701 tggggaagga gaaacgcaat ggatctggag ctggcgtggg catggtcagc gccagcaatc
     5761 ccaggtttgt ctcgggcaga tcttccgttt ccgtcagcgg ggtgggtgtt gggggtgaag
     5821 ccgtcggaga tgggatggaa ttcatttcga tttcaggcgt gggttccatc tcggtgggag
     5881 gcggaggtgc tgaccaaggg gattggcagc ctgccagcca gagcagcagt agccatctgg
     5941 ctgatttttt ccacctggaa atggttttag tattgcgagc agaggatctc atggtgttgg
     6001 ggagatggtc tgctgtaacg gcatgggtgt gcgtgtaatt gtcggtgtgc gggatgggaa
     6061 cggtgtgcgg gtgggcgtgg gagtgcgtgt cggacggcgt gtgggcgtgg gggtaaaggt
     6121 gggaggctga atgggggtgg gggtataaac agcctcaacc ggatagatct ctaccatcgc
     6181 agagaaccaa tccaggtcat cggtgtgtac gaactcgtta tagcgggctg ctggcagagc
     6241 tgagcgccat gtggacagcg cagggagacg ttcccagcca tagctggcgg ccaggttggt
     6301 gaagtccagc cagtagccgg gagggattgc cggtgcgagg ctgccacctt gttcatagtc
     6361 gcgtgggtcg ccagcatagc gcgcgttcag atcccaaggt atatcgtgaa gtggcatgcc
     6421 ctgtgatcca tcctggaagc gcgtcctcaa atacacgcgc cagtaagtgg tgggtccaaa
     6481 atcgtcgcgt atgatcacca tccagccagc attgatcggc gcgggattga taactatggc
     6541 tctacccgta tacagccagt cgttgagcaa gccagggaag agtggcgaag tcagcgggac
     6601 gtaggcgttt tctagggtgg cgaggaaatc ccagcccagg cgagaggcca cttctagccg
     6661 taatgcattg aaggattcat ctacgctatc gtgaagcttg gcgtatggtg cctgaacgtc
     6721 ttctaattct acgacccgtt gtcgaccggc gggtatgttg gttgcaggtg tcagttgagc
     6781 atctgccgca ggggtagtcg taatgcgtgc aacctggctc aggaaggagg gtagtggctg
     6841 aggtagtgag actctgcccc aggtcaagcc ggcaggctgc ccaggcaaag gcagcggcgg
     6901 cagggccagg ccggaagcat cagctcgata ggcggtgaga taggtgcggt ttggggtggg
     6961 aagcgtggtg agtacaatgt ccccatccgg actccaggcg ggccggttcc cgcctcccag
     7021 caggtgtggc tctgctcccg gctggtttat atccagcgac acaatattct gatagctgtc
     7081 gataactctc gtccacgaca acctggttcc atctggtgac caggctggat aggcatcgtt
     7141 ggctttggga ttgttgctga tattatggaa gcgatcatca atgcggtcca ggtcagccag
     7201 ccaaacctca ttttcaccgg tgcgattgga gacataggcg atttgacgcc ctgccggtga
     7261 ccagacagga gcataatccg cagcgggatc attcgtcaac cttatggccg gctggctacc
     7321 atccgcaggg cggattagga tttccaagtt ggggacagtc acggtgacag gcggggtggc
     7381 ggttatctgg gtggttgttg cttcactcaa cgttgcccct tgagggggaa gtgtggataa
     7441 cgtatctgta taactctcat aaacaagcca ttgcccatcc ggtgaccatg caggagcgcc
     7501 gtcgaattcc ggtgtgtctg tgaagcgtgt cgtcttgccc gtggagatat cgaggatgta
     7561 aatatcccaa aaaccttccc gggaggaggc gaaggccagg cgactgccat cagggctgat
     7621 ggagggatcg atatcgtccc aggttccgct cgtcatgcgg gtgaagggca agttttgtgg
     7681 gtggtaggca aagaggtgct tgtatccgcc ctcatcgata gacaagaaca aagtgcctga
     7741 attcggaatc aggtcagtcg cggatgcgct gctcgctcca gactgagaag ttatggatgt
     7801 tgcaccggga acaggtgtat cactgatctc tggaggggag attgtaggtg tgagtgaagg
     7861 agatattgct gtttggctgg cggtttcgct tgcaggcagt gctgtgttgg ctgtttctga
     7921 aggggtctgg ttgctagttg actggacctg agccagcgga cctcgaccat tgatccgccc
     7981 atcgagaaaa aatcccacca ccacaacagt gatgatcacc aacaaaacca tcagtacgcg
     8041 gcgtatctgc ctggtgatgg gatctccagt tggctcggat ggagaggtgt cattggcatt
     8101 tttcatgaag gttggctatg gcggatggat ttttatcctt ccgaacggag tcattctagc
     8161 atggtcacaa gagcgcgtca attgaagtta ttttatttgc tccgagcggg gaggtcctaa
     8221 caaaattgta tgttgtccat tgcaatttga gcaaaacatc ccctcgctaa tcttgcctgg
     8281 cgcgcattga ggcgtgtaat acagacattg gggtttgtat ggtctcagca cacaaacccc
     8341 aatgattcag acaaatccct tttcctggcg agggaggcgg gttactacaa tccgacggtc
     8401 agcaatggca ccggcgtgaa gaccaggaag aaaatcagca gacctagaat agcgattgcc
     8461 tggcgaggtg ggtctaaaga ggtgatttgg tcaagcggct cggcatggaa gcgacccagc
     8521 ataaaaatca ggaatgccca gagccaccat cctggccaaa cgagccccaa cagcaccaag
     8581 gcacccagga tgatcggtcg aaggaaactg gatttctttc ccaataacac atatagcaca
     8641 tgaccaccat cgagctgacc tgctggaatc aggttgagcg ctgttaccag caagcctgcc
     8701 caaccggccc aggctacggg atgaagcaga acatccagcc ctccaagtgg cagtgggcgc
     8761 cctgtgaaga agtagcgcac ccagtacatc caaggcggca ggttgccata attgcctggt
     8821 tccggcagga gtttgccaaa gaccaggtac ttggtgagca gataaattac cgagtttcct
     8881 tcgatctgca agccctgccc gggtggcaat tgagcaggga tggggttaac ttgcgagagg
     8941 gaaagtccca gtatcagcac tgggatggcc acgaccagac ctgcgagtgg gccggcgatg
     9001 ccgatatcca gcaagattcg ccggttcttg ggcggttctt ttagctggat gaaggcaccc
     9061 agcgttccga aagcgctgaa ggggaagggg ataaaatacg gcagggtgac atgtgtgcgg
     9121 tgatagcgcc ctgccaggta atgcccgaac tcatgcgcca ggaggatagc cagcaagctg
     9181 gcggcaaacg ccgcgcctcc ccccgtactt tgcagcacat aaggtaacaa gctgcggaag
     9241 gtgggattca tcgggccatc gtaggtgccg agcagtgtgt aatacgttcc agccagaaag
     9301 acggagatca ccgtcagcag gaacaatacc aaattgaccc aagattttga aggtttgggt
     9361 cgatttactc ccaatgccag aataaccaca tggctctctt gttctttgcg gaaataaggg
     9421 gtcacttcga gtgggcgcag ggctgaggcc agctggtcgt acgcggctgc agtgtcctcg
     9481 ctatatagct gccctcggta gcgggcgatg aagtcttttt tcgggtcgcc gcgcgtaata
     9541 tcttcaatct gcaacactcg cgcgacaatt gaggtcaggt tatcgaattc aagaggatac
     9601 atataacatc atcaatcgag taatcggttc tctatactca aacttaatca tcagataata
     9661 atatggcggg agtggatggg agtcgaaccc accgcggccc gcaacgcgac ccgccaccgg
     9721 ttttgaagac cggggagccc accgggaccc aaccactccc ccaggaacgg gacacgcgga
     9781 ttatatcaga ttagatattt aaataaatta acgtcctatc agattaatat agatataata
     9841 cggctatgta tgattccagc tccacctccg atgttcaccc agagaatgat tcccaagaca
     9901 agcaaacgca gagctcccaa gaatccgatg ccgtatggac tttccgtggg tatcgcttga
     9961 agtcaagcga atttaccacc gccatggtgc acttctttcg cgccgagatt tcgcgcgcca
    10021 acgtgtggcg gacgcggctg gatgctacaa ctaattgggc agtggtgacc actggcgcga
    10081 ctatcaccat tgcatttaac cccagtggat atcatggggt gatcatatta aatacgctgc
    10141 tgatcactct ttttcttttc attgaagccc ggcgttaccg ttattatgag ttatggagct
    10201 accgcgtccg cctcatggag accgatttct ttgcctctat gctcgtgcca ccattccatc
    10261 ctgcacccga ttgggcagag gctttggcag aaaacttgct acaccctgat tacccgattt
    10321 ctatgtggga agcatttggt aggcggttcc ggcgaaacta tatgtggatt tacattatcc
    10381 tggcggttgc atgggtggcc aaactttggc tcttaacacc tggcccgatg acatcctggg
    10441 ctgatttttt agccgcagcc tcaatcggct ccctttcagg cttgatcgtg ttgcttattg
    10501 gggtggtttt taatgccgcc ttgttcctga ttggcgtgct taccaccacc ttgcacaagg
    10561 ctactgggga ggtgctgccg cgctatatcc aggatatcaa aattcttggt ccagctactg
    10621 ccacagggca agatacggaa agccggcgtg cctggtttcg ccaatcccgc aggcgccagc
    10681 aactactggc atttgtcgtt acagatcaag ctcagattgt aagtcaggaa attctaaagg
    10741 agatggcacg gggtgtgacc tcgttggaag gggtgggtat gtacaccgga aagagacatt
    10801 ctgttctgat ggttgcctta accaccactg aagtagctca gctgaaagct ctagtgagta
    10861 aaacggatgc gaacgccttt gtgatcgttt ccccagccca agaggtactg ggtcggggct
    10921 ttataccgct taagaccaaa gaaacggaac gagagaattg atttcagcct atgccctctt
    10981 ggtataatgc gatacctaca tcggatgtta atgtatgaat gagacacacc agattgtccg
    11041 gcgcttaagc cgggttcagt ggattatcct cagtttgggt atcttaatca cctctggagt
    11101 tgagcttttc cagtacatgc gcgggggaga tttggtggag gttcttgtag agtggctcat
    11161 tgaggttttt ttcttaactc tgctgctggt agttgctata agattgtatg caaaggtgaa
    11221 ccaacaacta aagcgcagcc aagctgtgca tccgcgccag ggctcattaa tccagctgag
    11281 caccaaactg gcggcttcgc atgataaaga agagatctgc cacattttgg ttaaagaact
    11341 gcaggagctg atcgatcatg cgaaaattga gatttttctg atcgatcatg ccagcggtaa
    11401 ccgtagacga tatggaatta ccaacgaggg gcatagcgaa gagcctgttg attcagcgcg
    11461 tcctacccgg ttggagattc cgctgcgggc tgggcaagag cggctaggtg gattgctcgt
    11521 tgaggtcgaa caggcgcgcg gggtcagcga agatgaacac gcaattctgg tttctgcggc
    11581 aaatctgg
//
LOCUS       DBNGSX010000225         4002 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1541101, whole genome shotgun sequence.
ACCESSION   DBNGSX010000225 DBNGSX010000000
VERSION     DBNGSX010000225.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1541101"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="AC16CB_06780"
     CDS             complement(<1..727)
                     /locus_tag="AC16CB_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HIR3342208.1"
                     /translation="MTVALHPENNGKERIGVYVCHCGSNIAGTVNVEDVSHWASERLH
                     DRGVVIARDYKFMCSSLGQELIENDIQEMGLTRVVVAACSPHLHEQTFRSATARAGLN
                     PYLCELVSIREQVSWVHTDKTAATEKSKAVISGGVERVIHHEPLEPLHVPIHPATLVV
                     GGGIAGIQSALELADAGYPVYMVEREPSIGGHMAQFDKTFPTLDCSACILTPKMVSVG
                     THPNITLLTWSEVTKVEGYVGNFT"
     gene            complement(724..1713)
                     /locus_tag="AC16CB_06785"
     CDS             complement(724..1713)
                     /locus_tag="AC16CB_06785"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HIR3342209.1"
                     /translation="MNKYLFYPGCSMEASARAYNVSLMAICETLDLELEEIHDWNCCG
                     ATEYLGISLTPAYALISRNLALAAKQANGSHTVVAPCSACYLNLSKADHFMLERPSLG
                     ENVNLALEAGGLHYTPGSLEIRHLLDVFINEIGLDTIKSKVVKPLAGLRVAPYLGCMV
                     PRPDYEHRWSDHEHPNELDNLLKALGAEVIDFPLKTHCCSGHMPQISPSTAFELLRRL
                     IHSAAQYNADLMVTVCPMCQMNLDAFQHEMNRFYRTNYHMPSVFFTQLIGLAFGIKAA
                     ELGFGKELVDPRPALDRIGVEIPVSEETKARPKRTKKTGLPMPIMPGDEEMNE"
     gene            complement(1710..2309)
                     /locus_tag="AC16CB_06790"
     CDS             complement(1710..2309)
                     /locus_tag="AC16CB_06790"
                     /inference="COORDINATES: protein motif:HMM:NF024581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3342210.1"
                     /translation="MTTSIQETQLISETSEIPNLRFLEEVSAATAGVARLEMCIQCGT
                     CGGSCPSAADMDHTPRMLFAMIRAGLRDDALRSNTPWICVSCYHCVVRCPQDIHITDV
                     MYTLKSMAIQAKLYKDAKAPDFSQTFVDMVENFGRSYELGLATRHYLKHFPLRLPGMA
                     PMGIGLITKKRMDITPHRIDGIDQLRAILARAKQLEGIS"
     gene            2655..>4002
                     /locus_tag="AC16CB_06795"
     CDS             2655..>4002
                     /locus_tag="AC16CB_06795"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR3342211.1"
                     /translation="MSIEVDLQTRAVNTLRFLSADSVQKANSGHPGLPMGAAAMAYSI
                     WMRHMKFNPSNPSWPDRDRFILSGGHGSMLLYSLLYLTGNMLSLEELKNFRQWGSLTP
                     GHPEFGLTPGVETTTGPLGQGFANGVGMAIVEAHLSAIFNRPGHPVVDHYIYAIVTDG
                     DLMEGVASEAASLAGHLRLGRLIYLYDDNHITIDGSTDLAFTEDRAGRFEAYGWHIQR
                     VEDGNDIDAIDRAILRAKEDVRPSLILCRTHIGYGLPTKQDTEKAHGEPPGDEELDGA
                     KQKLDWPLQPRFYVPDDVMVHYRESIVRGEQAEVAWRNRMNAYRAEYPELADELERRY
                     RGDLPKIWDAGLPEFAVDNKGMATRIASSKILNAIAVKIPELIGGSADLAPSNKTWID
                     GSLSFQADTPQGRNFHFGVREHAMGSIVNGMALHGGVIPYGGTFLVFSDYMRPAIRL"
BASE COUNT          997 a          920 c         1041 g         1044 t
ORIGIN      
        1 ctgtgaagtt gccaacatat ccttctacct tggtcacctc actccaggtg agcagagtga
       61 tattcggatg tgttccaacc gataccatct ttggggtcag gatgcaagca gagcaatcta
      121 gcgtgggaaa ggttttatca aactgagcca tgtgaccgcc aatggatggc tcacgttcga
      181 ccatgtagac tggatacccg gcatcagcaa gctccagggc tgattgaatt ccagcgattc
      241 cgcccccaac taccagcgta gcgggatgta tcgggacatg caatggctcc aaaggctcgt
      301 ggtgtatcac gcgttcaacg ccgcctgaga ttactgcttt cgacttctcc gttgcagcgg
      361 ttttgtcggt atgtacccag gagacctgtt cgcgtataga aaccaactca cacagatacg
      421 ggttcaagcc agcccgggcg gtggcggaac gaaaagtctg ttcgtgaagg tgtggcgagc
      481 aggcagctac gacgacacgg gtcagtccca tctcctggat gtcgttttca ataagttctt
      541 gccccaggct tgagcacata aatttgtaat cgcgagcgat gacgaccccg cgatcatgca
      601 atctctcact tgcccaatgc gacacatcct ctacgtttac tgtgccggcg atattactgc
      661 cgcagtggca tacatagaca ccaatgcgct ctttgccgtt attttcggga tgtaatgcta
      721 ctgtcattcg ttcatctcct catcgcctgg cataatgggc atgggaagtc cggtcttctt
      781 agttctctta ggtcgagctt tggtttcttc ggaaacaggg atttctactc ctatgcggtc
      841 caatgctggc cgaggatcaa ccagctcttt accaaagcca agttcggcgg cttttattcc
      901 gaatgctaaa ccgatgagct gggtgaagaa tacacttggc atatggtagt tcgtacggta
      961 gaaacggttc atctcgtgtt ggaatgcatc caggttcatt tggcacattg ggcagacggt
     1021 caccatcaag tctgcgttat attgagcagc tgagtgaatt aaccttctca gcagttcgaa
     1081 agccgtagat gggctgattt gaggcatgtg tccgctgcag caatgtgtct ttaatgggaa
     1141 gtcgatcact tctgcaccca gtgcttttag aaggttgtcc agttcattcg ggtgctcatg
     1201 atccgaccaa cgatgttcat aatcagggcg aggtaccatg catcccagat agggagcgac
     1261 tcgaagtcca gcgagtggtt tgactacttt gctttttatc gtatccaagc ctatttcatt
     1321 gataaagaca tccagtaaat ggcggatttc caacgatcca ggcgtataat gtaatccgcc
     1381 ggcctccaag gcaaggttga cgttctcacc gagtgatggc cgttcgagca taaagtgatc
     1441 tgcctttgat aggttgagat agcaagcact gcacggtgcg acaactgtgt gtgagccatt
     1501 cgcttgcttg gcagccaatg ccaggttacg gctaatcagc gcatacgcag gagtcaggct
     1561 tattcccagg tactcggtag cgccacagca gttccaatca tgaatttctt ccaactcaag
     1621 gtcaagtgtc tcgcaaattg ccattaaaga aacgttatag gctctagcac tggcttccat
     1681 ggagcaaccg ggataaaaca gatatttgtt catgagatcc cctccagttg tttagctcgt
     1741 gccaggatag ctctgagctg gtcgattcca tcgatacggt gcggggtgat atccatgcgc
     1801 ttttttgtta ttagtccgat acccatcgga gccatacccg gcagacgaag gggaaaatgt
     1861 ttaaggtagt gccgtgtggc aaggcctaat tcgtagcttc tcccaaaatt ttcaaccata
     1921 tcaacaaaag tttgggagaa atcgggggct ttggcatcct tataaagttt ggcttggata
     1981 gccatggatt tcaatgtata catgacatca gtaatatgta tatcttgtgg acaacgtact
     2041 acgcagtgat agcaggaaac gcaaatccag ggtgtattgc ttcgtagtgc gtcatcacgc
     2101 aatccggctc gtatcatggc aaagagcata cgcggagtat ggtccatgtc ggccgctgaa
     2161 gggcaagacc ctccacaagt gccacattga atacacatct ccaggcgtgc aactccggca
     2221 gtggctgcac ttacttcttc caggaatctt aggttgggta tttccgaagt ttccgaaatt
     2281 aattgggttt cttgtataga agtggtcaaa agacacctcc ttcgttggtg ctttgatgga
     2341 gcgatttaga ttattcgaag gcatgcggtg aagggagaat tatcaaagta gagaaggaat
     2401 acaacctccg ttagaaactt acctattaat caatgcgaaa caacatcccg cccgaaaacc
     2461 atattgaatc tagtatcggt aagatgactg ctaaagtata gtgacgtatt tcacaaacgc
     2521 agaatgacat aagttactac ctaaaattta ttgatttatt tgattgcttt tgctacaatg
     2581 gagttcactt cttgccagcg gatgtttatg gggtagggga gagtgctgga aatggatacc
     2641 atgtggagtt ttggatgagc atcgaggttg accttcaaac tagagccgta aacacactgc
     2701 gttttttatc tgcagatagc gtgcaaaaag ccaacagcgg ccatcctggc ctgccgatgg
     2761 gtgctgctgc aatggcatac tcgatttgga tgcggcacat gaaatttaat cccagcaatc
     2821 caagctggcc ggatcgcgat cgcttcattc tttcaggggg acacggctcc atgcttctgt
     2881 acagcttgct gtacttgact gggaatatgc tgtctttgga ggagttgaag aattttcgac
     2941 agtgggggag tcttacgcca ggtcaccctg aatttggctt aacgccaggt gtagaaacga
     3001 ctactggccc cttaggtcaa ggctttgcta atggcgtggg gatggcaatt gttgaggcgc
     3061 atttatctgc catatttaat cgccctgggc atccagtggt agatcactac atatatgcca
     3121 ttgtcactga tggcgatctc atggaaggtg ttgcatccga ggctgcctca ctggcgggac
     3181 atttacggct gggacgcttg atatatctgt atgatgacaa tcacattacc atcgacggta
     3241 gcactgattt agccttcacc gaggataggg ctggacgttt tgaggcttat gggtggcaca
     3301 tacaaagagt tgaagatgga aatgatatag atgctattga ccgagcgatc ctgcgagcaa
     3361 aggaagatgt acgcccgtca ttgatcttat gtcgcacgca catcggatat ggcctgccta
     3421 caaagcagga tactgagaaa gcgcatggtg aaccaccggg tgatgaagag ctagatggtg
     3481 ccaaacaaaa gcttgactgg cctttgcaac cacgatttta tgtgccagat gatgtaatgg
     3541 tgcattatcg ggaatcaatt gtgcggggag aacaagcaga ggtagcatgg cgcaaccgca
     3601 tgaatgccta ccgggctgag tatccagaac tcgccgatga actggagcgg cggtatcggg
     3661 gcgatttgcc aaaaatatgg gatgccggtt tgcctgagtt tgctgtcgat aataagggca
     3721 tggcaactcg tatcgcatcg agcaaaatct tgaacgcgat cgccgtgaag ataccagaat
     3781 taatcggtgg atcagcagac ctcgcgcctt caaataaaac ctggatcgat ggctcgctga
     3841 gctttcaagc ggatacgccc caggggcgca acttccactt tggggtccgt gagcatgcca
     3901 tggggtccat tgttaatggc atggctttgc atgggggagt gataccatat ggcggtacat
     3961 tcctcgtttt ttctgattat atgcgcccag ccatccgctt at
//
LOCUS       DBNGSX010000226        11406 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_780337, whole genome shotgun sequence.
ACCESSION   DBNGSX010000226 DBNGSX010000000
VERSION     DBNGSX010000226.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11406)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11406)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11406
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_780337"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="AC16CB_06800"
     CDS             complement(<1..1139)
                     /locus_tag="AC16CB_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342212.1"
                     /translation="MPKDTNPHPFESDRSRLGFHYFPDTVHYRESDLRTWLPELKKMG
                     AAWLTLCAPPDRAIPEIFLRGLMRDGIEPILHFHLPLSHQFQDGTLGLLFRAYASWGV
                     RYTVLFDRPNQRSAWPASTWAQSDLVERFLDIFLPVANAALQAGLTPVFPPLEPGGDY
                     WDTAFLRAALQGMERRDQAELLEALHLSAYAWVDEHPLEWGEGGPERWPGAHPYFTPP
                     DQQDQRGFHIFDWYLTEAQAALGKPMPIFLLAAGRRLHNTDESPATDIDEIEHANINL
                     SIAKQFMAGASSDGVREPISPYILACNFWLLTANPSDPSANQAWFQPDDYHLPIVTAL
                     NKLVRPGDAQQPDQPTPAHQPESEPDLHPPSSTLEDTHPSPVEPK"
     gene            1463..3001
                     /locus_tag="AC16CB_06805"
     CDS             1463..3001
                     /locus_tag="AC16CB_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342213.1"
                     /translation="MSKSSTKVLVATFLIMALMISACEDTSFVGDPLLSQVHLIDPTF
                     REFYDLFGAEETLGPGISPLFAHRNIYYQYTTAALLMHDPGAPASQRFSLAPLGMDMD
                     IDDPPVSPPDDPKQRYVEGHIIFNLFVPLYEELGGARYVGVPISEVHYNPEKRRYEQF
                     FANLGFYWLEGDSPDQVHLLAYGAWKCDAYCRSPHPESSVVILPHHTAKRFNGAVDRL
                     GADFTGRAITDEYATPDGFNEQVFENVVMVVDPEQPSRVMLRSITERLGYMPDPMVAP
                     SQPKDYSFYEVQANRGYNVPKRFEAYIIQHGGYEVSGAPIGEIAHLKDAVYQQCFTNL
                     CIQAFLDALGNIAVRPAPLGYTYKLLPIQAVIQSPAQPITLPEVALPVEEVQAEPDVQ
                     VADQPMPEPTVETFTDDQVGQIVVQVWESFPMVSPNQNQEIGVSVFQNNQPVLNVEPD
                     LTIQLPDGSTRQYYMYPTGNDGQTRMTIEPIDAPGGTLIPYKVCIYGLGGQAYCTQDS
                     FLIW"
     gene            3290..3790
                     /gene="sodN"
                     /locus_tag="AC16CB_06810"
     CDS             3290..3790
                     /gene="sodN"
                     /locus_tag="AC16CB_06810"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HIR3342214.1"
                     /translation="MIFKVFEALDKRFHFERASAHCDIPCGIYDPSPAQIAALTVVRM
                     VDLMADLEAKGGEKDRAYTNSMGRYVAVKEEHAEKAKHEIRIIWGDYIKPQHMEKHPQ
                     LHDLVHKIMQYGSKSRQTADRDAAVQFVDAINQFSEIFWDTKGVKTKRAKAPYNPPLE
                     MVYPDL"
     gene            3790..4092
                     /locus_tag="AC16CB_06815"
     CDS             3790..4092
                     /locus_tag="AC16CB_06815"
                     /inference="COORDINATES: protein motif:HMM:NF021981.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HIR3342215.1"
                     /translation="MLRVIQVTGDSLSPEYREGDYVVITTIPFFLNRIQVGDVIVFQQ
                     PPFGTMIKKVERILGLGDGFYVSGTHQQSVDSRRYGSIPREALVGKVIWHILKPSR"
     gene            4142..5029
                     /locus_tag="AC16CB_06820"
     CDS             4142..5029
                     /locus_tag="AC16CB_06820"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3342216.1"
                     /translation="MIFGILTISTGSIMVRYAQSYAPSLVVAALRLFLATLVLTPFAL
                     SRHRSELAALSRGELALAGLSGFFLALHFAFWIISLEYTNVASSVVLATSTPLYVALF
                     APFTIKERVSRIILLGVALALVGGVVVGLSDACSINQAGLQCPELGQFFRGKAIIGDI
                     LATMAAVVAAAYVLIGRRLRAKLSLIGYISLVYGVSAIVLLVVMFASGNSPFGFPPMA
                     YFWFLMLALVPQLLGHSSFNWALGYASAALVSVALLVEPIGATLLAYIFLGETPTMLK
                     IFGAILVLIGVYIASRGEK"
     gene            5130..7388
                     /locus_tag="AC16CB_06825"
     CDS             5130..7388
                     /locus_tag="AC16CB_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013727.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR3342217.1"
                     /translation="MKNPNPAPNTQTAPTESTGMVWSRFRRFAWDIAGVATLAFALMT
                     LLALMLPQLTSGVLLAWWADVLWLWFGWGSLWVVVLLTVAGLLLLRRTTLAKNGEMLR
                     LNWGRVIALEMVAFASLAWLAAWGGSSFARADVGLDGGRIGWALSYLLGELLSPLGMS
                     GSIWQMLILSGVMIVGVILGFGLNRQLAAWVDQQVVTSQYSQADELGGEGAVSVAPIQ
                     GQRSGGATEAKKKKRMHLPVEFRQKFQVPADEASSDDAISPPDRDERLPPFDLLVHEQ
                     TARPDERTINETAYLIEKTLTEFGIPAKVVGFRIGPTVTQFAVEPGFVEKGSSNEGEA
                     NRQKVRVSQISALSRDLALALSAERLRIEAPVPGRPYVGIEVPNARSTVVRLRPVLES
                     ESFIKISSPLAIALGRDVSGQPVVTDLSRMPHLLIAGTTGSGQSVCIAALTTCLAMNN
                     TPEDLRLVMIDPKMVELVRFNGLPHLYGRVETDLERILGVMRWTVAEMDRRYRLLEAT
                     RSRNLDTYNRKMRRRKGEAPLPRIVVLIDELADLMMSTPDQIEPTLVRLAQMARATGI
                     HLVVATQRPSTDVVTGLIKANFPARLSFAVASSVDSRVILDTVGAETLLGHGDMLFLP
                     PESPGPMRAQGVMVSDQEVERVITFWQKSFPQAEVEPPPWEEMLKQESMLADRDDLIE
                     QAIKIVRENQRVSASFLQRRLRIGYPRAARLIDELEELGIVGPSQGGGREREVFIDPD
                     EGEEFQDYHVED"
     gene            7510..8127
                     /locus_tag="AC16CB_06830"
     CDS             7510..8127
                     /locus_tag="AC16CB_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR3342218.1"
                     /translation="MSENVVNQKKPLTLTDYLRVQFKWLLDSIAAFLNRLGLMPNTMT
                     ILGLIGNTLGAFFLARGQMTLGGILILLMGPVDALDGAMARLRGEPTEFGAFVDSVTD
                     RYSELVIFGGLIYFYMQRGDWLMVALAYAAAAGSVLVSYTRARAQSLGLETKVGLLTR
                     LERYLVLSPALVFNIPHIALWILAFLTNLTALQRIFDVRSKARTK"
     gene            8179..8976
                     /gene="lgt"
                     /locus_tag="AC16CB_06835"
     CDS             8179..8976
                     /gene="lgt"
                     /locus_tag="AC16CB_06835"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR3342219.1"
                     /translation="MPSQGFYVGPVFIYYYGIILMLGAVAASYLAEREARRRGQNGEL
                     VWDALIWVLIAGIVGARLWHIFTPPPSMVEQGITTKFYLTHPLDALWIRRGGLGIPGA
                     VIGGVVALYWFCRRRKLNFAEWADIAAPALALGQAIGRWGNFTNQELYGAPTSLPWGI
                     TIQYAPGYPPETRFHPLFLYESLWNLANMFLLLWLARRFSDRLKVGDLFLVYLIIYPL
                     GRFLLEFMRLDSALVGGININQTIMVVVALLAAGVLYWRHRKAVSAV"
     gene            9110..10048
                     /locus_tag="AC16CB_06840"
     CDS             9110..10048
                     /locus_tag="AC16CB_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3342220.1"
                     /translation="MIVTHQLGKQFDDFWAVDEVSLHVKAGEVLALLGPNGAGKTTTV
                     RMLTSILRPTRGEARVAGYDVVTQAARVRASVGVLTEQHGLYGRMPAGDYLDFFGEVY
                     GLDRSVRQARILDLLENFGLLSDRDRRIGEFSKGMRQKLALARALLHDPPVLLLDEPT
                     SAMDPESARLVRDSIRELRSASRAVIICTHNLSEAEELADHIAIIRRGRIIAQGSPQT
                     LKANFLGPAEYEVRLGVNLNGQVLSLPHGAKLTSQGEDWLRFRTLEPALANPAILRYL
                     LDLGLPVLGLQEVARSLEEVYLKAVSVAEGEEGTDV"
     gene            10041..>11406
                     /locus_tag="AC16CB_06845"
     CDS             10041..>11406
                     /locus_tag="AC16CB_06845"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA];
                     GO_process: GO:1901893 - positive regulation of cell
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HIR3342221.1"
                     /translation="MFEAARQQLMPALIITRREVRDQFRDWRIIFPIVILTIFFPVLM
                     NFTAKQAISFVERYGAPIVGDRLIPFLLMVVGFFPISVSLVIALESFVGEKERYSIEP
                     LLCTPLSDWQLYLGKLLAATIPPLLASYLGIGVYVVGIFTQVGWQPDFLLLTQIVLLT
                     TVQALVMVCGAVVISSQTTSVRAANLLASFIVIPMALLIQGESIIMFWARYNVLWWVI
                     FGQVLIAGLLLRVGITKFNREELLGRELDTLNLRWSWRIFKSKFIGQARSPLTWYRLE
                     IAGTVRAMTVPIGIMSLALIAAIMIGVEQARVFVIPRELLNFPDIEQGFISGLEYIRL
                     FSVSGIGTVWFHNLRAIVIATTMGIFSFGILAVVILMLPLMLVGYFMANLAQTGISPF
                     LLFSTLVLPHGILEIPAIILAGASILRLGATLAAPSQGETIGEAWLRSLADWAKIMLG
                     LVIPL"
BASE COUNT         2449 a         2779 c         3176 g         3002 t
ORIGIN      
        1 attttgggct caacaggtga tggatgcgtg tcttccagcg tgctgctggg cgggtggaga
       61 tcaggctcgc tctccggttg gtgagctggt gtgggctggt ctggttgctg agcgtcccca
      121 ggtctcacca gtttgttcaa cgccgtcacg atgggaaggt gataatcgtc tggctgaaac
      181 catgcctggt tagctgaagg atcagaagga ttggcagtga gcagccaaaa attgcatgcg
      241 aggatatacg gtgatatcgg ttcccttacg ccgtcagatg atgcacccgc cataaactgt
      301 ttggcaatgc tcaaattgat gttggcatgt tcgatctcat cgatatctgt cgctggggat
      361 tcatcggtgt tgtgcaggcg gcgacccgca gccagcagga atattggcat cggcttgcca
      421 agagcggctt gagcttcagt tagataccaa tcgaaaatat gaaagccgcg ttgatcttgt
      481 tgatctgggg gagtgaaata tggatgcgca ccaggccaac gctccggtcc gccttccccc
      541 cactctaatg ggtgctcatc gacccaagca taagcgctca ggtggagggc ttccagcaat
      601 tctgcctggt cgcgccgctc catcccctgc aaggcagctc gaaggaaggc tgtatcccaa
      661 taatccccac cgggttcgag cggagggaag accggggtca atccggcttg taaggcggca
      721 ttggcgaccg gcaagaagat gtccaggaag cgttccacca gatcggactg tgcccatgtg
      781 gaggctggcc aggcgctgcg ctgattgggg cgatcgaaca agacggtata gcgtacaccc
      841 caactggcgt atgccctgaa taacaatccc agggtgccat cttggaactg gtgtgataaa
      901 ggcaagtgga agtgcaagat aggttcgatc ccatcgcgca ttagaccgcg caagaaaatc
      961 tccggtatcg cgcgatctgg tggtgcacac agggtcaacc aggcagctcc cattttttta
     1021 agttctggca accaggtgcg gagatcactc tctcggtaat gcacggtgtc tggaaaataa
     1081 tggaagccca ggcgtgaacg gtcactctcg aatggatggg ggtttgtgtc tttaggcatg
     1141 ccatgctcct ccccttaaaa gggggtttga cacctttatc gatgcaagag gtgtgccata
     1201 aagtgcgttt tttaggggat tcctgacaaa atcctgttaa aaacgtcgat tccttacaaa
     1261 agtgtcagtt ataatttaag gacagcagta tatactaggg agtggtcgtt aaggttacct
     1321 taaggacatc atgatacacc tgcaatttct cttgaaactt cttataagat agctgccgtt
     1381 gctcgcgctt tttatacgca tgtggttgga tgatccagtg ttaaagagca atagtttatt
     1441 cggttgtctc gagaaatatt aaatgtccaa atcgtcaacc aaagtgcttg ttgcaacttt
     1501 tttgataatg gctttaatga tctcggcttg cgaggatacc agcttcgtcg gcgatcccct
     1561 cttgtcccag gtgcatttaa tcgatccgac tttccgggaa ttttatgatc tatttggggc
     1621 agaggaaaca ctcggtccgg gaatttcccc tctctttgca cacagaaata tctattatca
     1681 atatactact gctgcattac tgatgcatga tcctggcgct cctgccagcc agcgtttttc
     1741 actggcacct cttgggatgg acatggatat cgatgatcca ccagtatccc cgccagatga
     1801 tcccaagcag cgctatgtcg aaggccacat cattttcaac ctgtttgtgc ccctgtatga
     1861 ggagctgggc ggcgcccgct atgtgggtgt tccgatcagc gaagtgcatt acaaccccga
     1921 aaaacggcga tacgaacagt tcttcgccaa cctaggattt tactggttag aaggcgattc
     1981 gcccgaccag gtgcatctgc tggcatacgg cgcttggaag tgcgacgcgt actgccgttc
     2041 tcctcatccg gagtcaagtg tagtgatcct gccgcatcac acggcaaaac gttttaatgg
     2101 ggcagtggat cgcttgggtg ctgatttcac cgggcgcgcc atcaccgatg aatatgcaac
     2161 ccccgacggt tttaatgagc aggtcttcga aaatgtcgtg atggtagtcg accctgaaca
     2221 acctagcaga gtgatgttgc ggtcgatcac agagcgcctc ggttacatgc ctgacccgat
     2281 ggtagccccc agccagccca aggattattc tttctatgag gtgcaggcta atcgagggta
     2341 caacgtcccc aagcgttttg aagcatatat catccagcat ggtggttatg aggtgagcgg
     2401 ggcgccaatt ggtgagattg ctcaccttaa ggatgcggtt taccagcaat gttttaccaa
     2461 tctctgcatc caggcttttc tggacgcgct gggcaatatt gccgttcggc ctgcacctct
     2521 gggttataca tacaaattac tgccaattca ggcagttatc caaagtccgg cgcagcccat
     2581 cacgttaccc gaagtagcat tacccgttga agaagttcaa gctgagcctg acgttcaggt
     2641 ggcggatcaa cccatgccgg agccaactgt tgagaccttc accgatgatc aagttggtca
     2701 gattgtagtt caggtctggg agagcttccc gatggtttct ccaaatcaaa atcaggagat
     2761 cggggtgagc gtatttcaaa ataatcagcc agtgcttaat gttgagccag acctaaccat
     2821 acaactgcct gatggcagca ccaggcaata ttacatgtac ccaactggca acgacggtca
     2881 gactcggatg actattgaac cgatcgacgc gccgggcggc acattaatcc cttacaaggt
     2941 gtgcatttac ggcctcggtg gacaagctta ctgcacccaa gatagcttcc tcatttggtg
     3001 atatccctag accgcgaaaa tttctaaatc ctttttcacc atattcccct cgctcctcgc
     3061 aggggaatat ttttaatttg catgcttccc aaggttcgaa aacttgaact tcaaaaccaa
     3121 taagccagcc accagctctt ccgtgtgcta tcgaccttgt caaaactcta tgactcagac
     3181 aattaatctt gacaggagag gtctttatca ttatgatcat ttgtgtcttc taccgtgtag
     3241 aagtatccaa aataatgtaa caagataatc acttatgaag gaggattgta tgattttcaa
     3301 ggttttcgaa gctttagaca agcgattcca ttttgagcgc gccagcgccc actgtgacat
     3361 cccctgcggc atttatgacc ccagccctgc acagatcgct gcgctgacgg tagtaagaat
     3421 ggtggattta atggccgact tggaagctaa gggtggtgaa aaggatcgag catacactaa
     3481 tagtatgggc cgttatgttg cggtaaaaga agagcatgct gaaaaggcca agcacgagat
     3541 tcgtattatc tggggtgatt acatcaaacc tcagcatatg gagaagcatc cccagctgca
     3601 cgacctggtg cacaaaatta tgcagtatgg ctcgaaaagc cgccagaccg ctgatcgcga
     3661 tgcagcagtc cagttcgttg atgctatcaa ccagttttcc gagatcttct gggataccaa
     3721 aggcgtgaaa accaagcgag ccaaggctcc ttataatccg ccattagaga tggtttaccc
     3781 tgatctgtaa tgcttcgcgt gatccaagtg acgggcgata gcctgtcacc agaataccga
     3841 gaaggggatt acgtggtaat caccacgatc cccttctttt tgaatcgaat ccaggtgggt
     3901 gatgtgattg tttttcagca gcctcccttc ggcacaatga tcaaaaaagt agagcgcatc
     3961 ctgggattgg gggatggttt ttatgtatcc ggcacgcatc agcaaagtgt ggatagccgg
     4021 cgatacgggt cgatcccgcg agaagccctg gtgggcaaag tgatttggca tatcctgaaa
     4081 ccatctagat aacatgcagc ccccatcgag tcgacaccct ccgctacctc ccatggcggt
     4141 gatgattttt ggtatcctga cgatttccac cggctcgatt atggtccggt atgctcagag
     4201 ttatgcgcct tcgttggttg ttgctgcctt acgcctattc ctggctacgt tggtattgac
     4261 tcccttcgcg ctatcacgtc accgtagcga acttgcagcc ctgtcgcgcg gcgagttagc
     4321 cctggcaggc ttatccggct ttttcttggc gttacacttc gctttctgga ttatctcgct
     4381 ggaatatact aatgtcgcta gctcggttgt gctggctacc agcacgccgc tgtatgttgc
     4441 gctgtttgcc ccatttacga taaaagagcg cgtcagccgc attatcctgt tgggcgtcgc
     4501 actggcgttg gtaggtggag ttgtggtggg attaagcgat gcctgtagca tcaaccaggc
     4561 aggcttgcaa tgccctgagc tggggcagtt cttccgtgga aaagccatca tcggtgatat
     4621 tctcgccacc atggcagcgg tggtagcagc ggcatatgtg ttgattgggc gacgactacg
     4681 tgcgaaactg tctctcattg gctatatcag cttagtgtac ggcgtgtcgg ctattgtgct
     4741 gctagtggtt atgtttgcca gcgggaactc gccatttggc ttcccgccaa tggcttattt
     4801 ctggttctta atgttggcgt tggtgccgca attgctgggg cattcctcct ttaactgggc
     4861 gctcggatat gcctctgctg ccttagtctc cgttgccttg ctggttgagc caattggcgc
     4921 tacgctcctg gcgtatatct tcctcgggga gacgccgacg atgctcaaga tatttggtgc
     4981 tatacttgtt ttgatcggtg tctatatcgc ctccagaggt gagaaatagc catcgattgg
     5041 catgacaaca gtcggagatg gagcattaaa gtgaggaaac attatcctac tctggcccca
     5101 atcccctacc tggcatgtgg gcgagagcga tgaaaaaccc caaccctgcc ccgaatacgc
     5161 aaactgcacc aacggaatca accgggatgg tatggtcacg cttccggcgt tttgcctggg
     5221 atatcgcagg tgtggcgact ttagcctttg ctttaatgac cttgctggcg ctgatgttgc
     5281 cacaactgac tagcggtgta ttgctggctt ggtgggcaga tgttttatgg ttgtggttcg
     5341 gttgggggag cctttgggtg gtcgtgctct tgacggtggc tggattgctc ttgctgcggc
     5401 gcaccactct tgcgaagaat ggcgagatgt tgcgactgaa ctgggggcgt gtcatcgctc
     5461 tggaaatggt cgccttcgcc agcttggctt ggttggctgc atggggtggt tcatcttttg
     5521 cgcgtgcgga cgtcggttta gatggtgggc gcatcggctg ggctctctcc tacttgctgg
     5581 gagagctcct ttcgccgctg ggcatgtctg gctcgatatg gcaaatgctg atcttaagcg
     5641 gtgtgatgat cgttggggtc attttgggtt ttggattgaa caggcaattg gctgcttggg
     5701 tggatcaaca agtagttacg tctcaatact cccaggcgga tgagctgggt ggcgagggag
     5761 cggtgagcgt tgcccctatt caaggtcagc ggtcaggtgg agcaacggaa gcaaaaaaga
     5821 agaaacgaat gcacctgcct gttgaattcc gccagaaatt tcaggtcccc gcggatgaag
     5881 caagcagcga cgatgcaatc tctccccctg atcgagatga gcgtttgcca ccatttgacc
     5941 tgcttgtgca tgagcaaacc gcgcgccctg atgaacgtac gatcaatgag acggcgtatc
     6001 tcattgaaaa gacgctcaca gaatttggca tcccggcaaa ggtggtggga ttccgtatcg
     6061 ggccgactgt gacgcagttt gctgttgagc ctgggtttgt ggagaaaggg tcctcaaatg
     6121 aaggcgaggc taaccggcag aaggtgcgtg tttcgcagat atctgccttg agccgcgatt
     6181 tggctctggc cctgtctgcc gagcgcttgc gtatcgaggc ccctgtgccg ggcagaccat
     6241 atgtgggtat tgaagttccc aatgctcgct cgacagtggt tcgattgcgc cctgttttgg
     6301 aaagcgagtc attcattaag atttcttcgc cactggctat tgccctgggg agggatgtct
     6361 ccgggcagcc ggttgttaca gatctatctc gtatgccgca cctgctgatc gctggaacca
     6421 cggggtcagg tcaatcggtt tgcatcgctg ctttgacgac atgcttggca atgaacaaca
     6481 ccccggaaga cctgcgcctg gtaatgatcg atcccaagat ggtcgagctg gtgcgcttta
     6541 atggcttgcc acatctatat gggcgggtag agacggatct tgagcgcatt ctgggcgtaa
     6601 tgcgctggac ggtggcggag atggatcgcc gctaccgatt gttggaagcc acacgttcgc
     6661 gcaatctgga tacctataac cgcaaaatgc gccggcgaaa gggtgaagcg ccgttgccgc
     6721 gcatcgttgt gctgatcgat gagctggctg atcttatgat gtccactcca gaccaaattg
     6781 aaccgaccct tgtgcgtctg gctcagatgg cacgcgccac aggcatccac ctggtggtcg
     6841 ccacccagcg tccgagcacc gacgtggtta ctgggttgat caaggccaac ttcccagccc
     6901 gtctttcctt tgcagtggca tcctcagtcg attcgcgggt aatcctggat acggtcgggg
     6961 ccgagacttt gctggggcat ggtgatatgc tcttcttgcc gcctgaatcc cctggtccaa
     7021 tgcgggcgca aggtgttatg gttagcgacc aggaagtgga acgggtgatc accttttggc
     7081 agaaaagctt tccccaggct gaagtcgagc cgcctccttg ggaggagatg ctcaagcagg
     7141 aatccatgct ggcagaccgt gacgacctga ttgagcaggc gatcaaaata gtgcgcgaaa
     7201 atcagcgggt aagcgcctca tttttgcagc gccgcttgcg gattggttat ccccgtgctg
     7261 cccgattgat cgacgaactg gaagagttgg gcatagttgg cccatcccag ggaggtggga
     7321 gggagcgcga ggtgttcatc gatccagatg aaggcgagga gtttcaagat tatcatgtgg
     7381 aagattaaag gctgggtggt catttctatg cttttatctg atgctcatat ttgattactt
     7441 gacatccaca tgacctgctg atagcatact ccgaatgcca ttatgcctca ttgaaaggtg
     7501 atctcgaaat tgtctgaaaa cgtagtgaat caaaaaaaac cattaaccct tactgactat
     7561 ctacgggtgc aatttaagtg gctgttagat agtatcgccg cgttcttgaa tcgactgggt
     7621 ttgatgccca atacgatgac cattttgggt ctgattggca atacgcttgg agcatttttc
     7681 ctggcgcgtg gtcaaatgac cctcggcgga atactgatct tgttgatggg gcccgtcgat
     7741 gcgctggatg gcgccatggc caggttgcgc ggagagccga cagaatttgg cgcttttgtc
     7801 gattcggtta ctgatcgcta ttcagagttg gtaatcttcg gcggtctgat ctacttttat
     7861 atgcaaagag gggattggct gatggtggca cttgcctacg ccgccgccgc cgggtctgtg
     7921 ctagtatcgt acacacgcgc ccgggcgcaa tccttgggtt tggagaccaa ggtggggttg
     7981 cttacccgtt tggagcgcta tctagtgctc tcgccggcat tggtcttcaa tattccacat
     8041 atcgctcttt ggatccttgc tttccttaca aatctaaccg ccttacagcg gatatttgat
     8101 gtgcgtagca aagctcgaac gaaataaccc ggggtagaag acacgataac aattagatcc
     8161 taattctgag ggagaaccat gccatctcaa ggtttttacg ttggaccggt atttatctac
     8221 tactatggaa tcatattaat gctcggcgca gtagcagcct catacctcgc agaacgtgaa
     8281 gcccgccggc gcgggcagaa cggtgagctg gtatgggatg cattaatctg ggtgttgatc
     8341 gccgggattg ttggggcgcg cttgtggcac atctttacac cacctccctc gatggtggag
     8401 caagggatta ctacaaagtt ctacttaacc caccccctgg atgcgctgtg gatacggcgt
     8461 ggtggtttgg gcatccccgg cgcggtgatc ggcggcgtgg tggcgctata ctggttttgc
     8521 cgaaggcgca agctcaattt tgccgagtgg gcggacatcg ctgcgcccgc acttgctttg
     8581 gggcaggcca tcgggcgctg gggtaatttc accaaccagg aattatatgg cgcgcccacc
     8641 agccttccct ggggcataac catccagtat gcgccgggat atccgccgga gacgcgtttt
     8701 catcccctct tcttgtatga gtctttgtgg aacctggcca atatgttcct gttgctctgg
     8761 ttggcgcggc gattctccga tcgcctgaag gtaggtgatt tgtttctggt gtacttgata
     8821 atctacccgc taggtcgctt cctgctagaa tttatgcgat tggattccgc cctggtgggt
     8881 ggtatcaaca tcaaccagac catcatggtg gtggtcgcct tgctggctgc tggcgtttta
     8941 tattggcgcc atcgcaaggc agtgtctgct gtttaatgat gatgtactgg tatttcaact
     9001 gtacatttcc ccactcatct aattacagga ctgttgtctt attgaaaaat tcggttgaaa
     9061 tgaatatagt aggttatgtg ggtgttatca tgcggggttc atacgaaaga tgatcgtaac
     9121 tcaccaatta ggtaagcaat ttgatgattt ttgggcagtt gatgaggtaa gcctgcacgt
     9181 gaaggctggc gaagtgctgg ctttgttagg tccaaatggg gcaggtaaga ccaccacggt
     9241 gcgcatgttg acctccatct tgcgacccac gcgcggcgaa gcgcgcgttg caggttacga
     9301 tgtagtaacc caagcggcac gtgtgcgggc ttcggtgggc gtgctgaccg agcagcacgg
     9361 cttgtatggt cgcatgccgg ctggcgatta ccttgacttc tttggggagg tatatggcct
     9421 ggatcggtca gtccggcagg cgcgcatcct cgatttactg gaaaattttg ggttgctatc
     9481 agaccgcgat cggcgcattg gggagttttc gaaggggatg aggcagaagt tggccttagc
     9541 gcgtgccttg ttgcatgacc ctccggtgtt gttgctggac gaacccacct cagccatgga
     9601 ccccgaaagc gcccgcctgg tgcgcgattc tattcgggaa ttgcgttcag ccagccgggc
     9661 agttatcatc tgcactcaca acctgtctga agcagaggaa ctggcagatc atattgctat
     9721 catccgcaga ggccgtatta tcgcccaggg atcgccgcaa actttgaaag caaatttttt
     9781 aggaccggct gagtacgaag tccgtttagg agtcaacttg aatgggcagg tgctttctct
     9841 cccacatgga gcgaagctga catctcaggg agaggactgg ctgcgctttc gtactttgga
     9901 acctgcgcta gctaatccgg ctatcctgcg ttacctgctt gacctgggct tgcctgtgct
     9961 ggggttgcag gaggtggcgc gcagcctgga ggaggtttac ttgaaagctg tcagtgttgc
    10021 tgagggtgag gaggggaccg atgtttgagg ctgcccgcca acagctcatg ccggcgttga
    10081 ttattacccg ccgtgaggtg cgtgatcagt tccgtgactg gcgcatcatc ttccctatcg
    10141 tcatcctgac catcttcttc cccgtactga tgaatttcac tgccaaacaa gcgatctcat
    10201 ttgtggaacg ttatggtgcg cctattgtag gcgatcgctt gatccctttc ctgctgatgg
    10261 ttgttggttt tttccccatc tctgtatcgc tggttattgc cctggagagt tttgtcggag
    10321 aaaaagaacg ctatagtatt gagccgctgc tgtgcacccc attgagtgac tggcagttgt
    10381 atctgggcaa gttattagct gcaacaattc cgcccttatt ggccagctac ctgggcattg
    10441 gcgtttacgt ggttggcata ttcacgcagg tgggatggca gcccgatttt ctattgctga
    10501 cacagatcgt gttactcact acagtccagg cactggtgat ggtgtgcgga gcagtggtta
    10561 tctcatccca gactacctca gtgcgcgcag cgaatctgtt ggcctctttt atcgtcatac
    10621 caatggcatt gttgatccag ggggagagca ttattatgtt ctgggcgcgc tacaacgtgc
    10681 tgtggtgggt tatctttgga caagtgctca ttgctggtct gttgttacgg gtaggaataa
    10741 ctaaatttaa ccgcgaagag cttttaggca gggaattgga taccctgaat ctccgctgga
    10801 gctggcggat attcaagagt aaatttattg gccaggccag gtcacccctg acctggtacc
    10861 ggctggaaat tgccggcact gtgcgtgcaa tgactgttcc aatcggcata atgtcattgg
    10921 cattaattgc tgctattatg atcggtgttg aacaagcgcg ggtgttcgtc attccccgcg
    10981 aattgttgaa tttcccagac atagagcaag gctttatttc aggactggaa tatatccgcc
    11041 tcttctctgt gtcagggatt ggcacggttt ggtttcacaa cctaagggct atcgtaattg
    11101 caactacgat gggtattttt tccttcggta ttctggctgt agtcattttg atgttgccgt
    11161 tgatgcttgt gggatacttc atggccaatc tggctcaaac tggtatatcc ccgtttttat
    11221 tattttccac gctggtgcta cctcatggca tactcgaaat ccccgctatt atccttgctg
    11281 gagcatccat tttgcgcctg ggagctaccc tggcagcgcc ttctcagggc gagactatcg
    11341 gcgaggcatg gttgcgttca ctggcagatt gggctaaaat catgctgggt ctagtgatac
    11401 cccttc
//
LOCUS       DBNGSX010000227         8248 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_294974, whole genome shotgun sequence.
ACCESSION   DBNGSX010000227 DBNGSX010000000
VERSION     DBNGSX010000227.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8248)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8248
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_294974"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="AC16CB_06850"
     CDS             <1..633
                     /locus_tag="AC16CB_06850"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3342222.1"
                     /translation="VLLTIMSACNPSLGRSEIEIVITQSPTPISDRLAEPALPANPTQ
                     YEQGRHLYWLNCMPCHGDKGQGLTDEFRSLWVDDHQYCWARGCHSGRPGEEGFPIPLT
                     VPAIISPSSEPPQFATEEDLFEYLSTTHPPQRPGYFSDDEYWAITAYVLTENNRLPRG
                     QKIGLQEEKIALEEIRLVAAIGLIFLIVSAIIAWFHRRHNMLRLPKTPEF"
     gene            1043..1765
                     /locus_tag="AC16CB_06855"
     CDS             1043..1765
                     /locus_tag="AC16CB_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342223.1"
                     /translation="MRIKRLLFIVILVALLVPLPTQAAWQGAPVEGRFRPRCRLPESN
                     AEALVILSNYYPGYWWDHTDLTVAVQAHPNASAAQLEAIHDAIATWGDVLDDCFRGLI
                     TVTDVTGTLKSEQKAADIVVHYVPTAGGVAFGGYAICGDHGCPNILVRSDLPPSLDRP
                     PYEPEYLGWVTLHELGHALGLGHTTNLLESTDLMGYGWPDLGDPVFSDCDIDALMFVF
                     AWLFEGSEPHPPAEGPYDCSLD"
     gene            2063..3196
                     /locus_tag="AC16CB_06860"
     CDS             2063..3196
                     /locus_tag="AC16CB_06860"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3342224.1"
                     /translation="MKLKIVVLGAGFGGLELSAILSEALSERLDLTLIDKSDTFYFGY
                     SKLDVMFGRKAPDSVKLAYKNIVKPGVRFRQETIMAIDPEARRVTTDSAAYDADILVV
                     ALGADYDQAATPGLIEGGNEYYSFAGAMRLREVLPTFSKGRAIVGVTSTPFKCPPAPS
                     EAALLLHDYLTKRGVRDNCEISLVMPFGIPIPPSPATSKALLAAFAERGISFIPNRLV
                     QALDPARHVAILDDQSEMPYDLFLGIPKHRVPAVVAASGMTEDGWVPVNPKNLKTRFP
                     GVYAIGDVTSVGTPKAGVFSEGAARVVAASLIAELQGSEQPSAYDGAGSCYIEFGRDL
                     VGRVDVDFLSGPSPTGSFSDPSLALVAEKAQFGSSRKARWFGL"
     gene            3486..4778
                     /locus_tag="AC16CB_06865"
     CDS             3486..4778
                     /locus_tag="AC16CB_06865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR3342225.1"
                     /translation="MTDATHSTREHRRDLDLLRVLLVFGLVFFHTARIFDTISLPEGV
                     KNQPPSIAATIFTAFFALWGMPLMFTVAGFAIWHSLSKRTGQAFVVDRIHRLVVPFVF
                     GVLALVPLQVYFHLKQAEPASRITYWQFLQLFFDVRLCPDLVNAFICPDPATGLFTTA
                     HLWFLKDLFIFSMLLLPLFLYLGGQTGGNVIGKLGDFLARPGMIWLVALPVAGFEAGF
                     VTSTMGGSWNEYTYAVLLMAGFLIAAEPRLAQVMGRVWRSGLVIGLVVEAVYMVGLYL
                     LMNVYDLDPLHGYDWGSLLWRGIKSVGAWAWIVAIIGYGSRPRSKPIPEGVRKSTGTG
                     NLVARAIAYAGEAFLAFYILHQTVIFVIGYYVVQWAIGALLKFIVISLSSLAVTLLLY
                     ELGVRRTKVTRWLFGMRINPPAPLADVPPGRSAGEVAV"
     gene            4888..5178
                     /locus_tag="AC16CB_06870"
     CDS             4888..5178
                     /locus_tag="AC16CB_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342226.1"
                     /translation="MIIVRNVFQAKYGKGDELVQLMKDSQAIWPSERKVRIMTDLSGP
                     FFTVVTEADYESFSAWEADAKKTFGDDRFAGLFERMMPLVESGSREFYTLVE"
     gene            complement(5265..6695)
                     /locus_tag="AC16CB_06875"
     CDS             complement(5265..6695)
                     /locus_tag="AC16CB_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342227.1"
                     /translation="MCTKKQRLNWNCGWILILLALASTACASQTGDVSIAEHATAQEI
                     TPKFAETPRDNNVPEALVDKVDESSCKGKFPSLSTGFDIQQTPSMDEPPAKVPFRDSV
                     FGTCMMRLTDRFEDLTSEDPSQGLKNEYSRVQSFNADGSRVLVRSIDAYWYVYDAQIL
                     KPMEHIPLSVEPRWDAEDPDLLYHIDETRLLAYRLSTAEERLVHDFAVDFPGQDLAAV
                     WTRYEGSPSFDGRYWGFMAEDQEWLATALLVYDLKIDRVVARRDLPSRPEIDSVSISP
                     LGGFFLAYYDGYCEPGQLGSDAHPCGLMVYDRDLQNGRGLLRIVGHSDLALDANQREV
                     LIFQDIDTDNISLLDLESGEVTPLWPIDFSHSALGLHFSGRASRMPGWALVSTYNGAQ
                     PAATWMDDQIFVVELKPGGRVVRLVHTYSIVDENQEHDYWAEPHASVNHDFTRIVFTS
                     NWGRSGTGEVETYLIELPPDWLSQLP"
     gene            7050..8027
                     /locus_tag="AC16CB_06880"
     CDS             7050..8027
                     /locus_tag="AC16CB_06880"
                     /inference="COORDINATES: protein motif:HMM:NF013087.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HIR3342228.1"
                     /translation="MMDLAGGIAFHALSVAIKLKIFEAIGDKSLPPSEIAHLISTDTR
                     ATGLLLDALEALGYVEEQQGSYSNTPMTLKWILETSPNSLACMISGMEGALDRWKYMD
                     ETIRNGSPSKLAWEWLDQHPGSWADYQALMIGMAHSCADEIVSRVKLPPTARKLIDLG
                     GGHGLYSIGFCRKYPGLTATVFDWPQAKDIANDTILSEGMNERVKFVVGDLWKDDYGS
                     GYDVALLFQIIHMYSPEKNIQLLTKVREALSPNGILVINDQVAVDAPSPLAKLFARLQ
                     SLELLNSVNGQTYPPVEIEGWLVEAGYSNPSTMLLRNTPGFGVVVATKP"
BASE COUNT         1940 a         2090 c         2152 g         2066 t
ORIGIN      
        1 gtgcttctca cgattatgtc tgcttgcaat ccttcactcg gtcggagcga gatcgaaata
       61 gtaataaccc aatctcccac gcccatctca gaccggctgg ctgagccagc cctgcctgca
      121 aacccaaccc agtatgaaca aggccgccac ctgtattggt tgaactgcat gccctgtcat
      181 ggcgacaaag gccagggctt gacagatgaa tttcgcagcc tgtgggtgga tgatcaccaa
      241 tactgctggg cgcgcggctg tcattctggc cgaccaggtg aagaaggttt tcccatcccg
      301 cttacagtcc ccgcaatcat ctcaccatcc agtgaaccac cccaatttgc gacagaggaa
      361 gatctttttg agtacctcag caccacacac cctccccaac gccccgggta tttctcagat
      421 gatgaatact gggcaattac agcttatgtc ctgacggaaa acaatcgcct gccccggggg
      481 cagaagattg ggctgcagga ggagaaaatt gcactagaag agatccgctt ggtcgcagcc
      541 atcggtctga tattcctgat cgtgagcgca atcatcgctt ggtttcatag gcggcataat
      601 atgcttaggc taccaaagac gccagaattt tgaaaactgt ggctcgcatc gttcctgaat
      661 tcaacctcga tgaagttaat tggtcgtcca ggagcgaatc gtttaccgac cgacgaattc
      721 tcttcgcctt tcagaacttc atctcgcgcc ggctcactac tccttggcac ggatttgata
      781 gagtaaggaa gctgaaatcc taaccatagc tctcaaggtc atccagcaca atgacgaagg
      841 catgctgagt tgatacgtgc ttcgcatctc tttattgaag cgttcaattt tttcattggg
      901 acgcctcttg acttttaata aattggcgat atgataatga tatacatcgt tgcagaggtg
      961 gatagatcaa ggaggaaatc caaaaccttt gcttcataag gccaagtatt tccattacct
     1021 aaatagacag aggaggacat aaatgcgtat caaacgatta cttttcatcg ttatccttgt
     1081 cgcactactt gtaccccttc ctacgcaagc ggcttggcaa ggagctcctg tagaaggtcg
     1141 ttttcgtcca cgctgtcgcc taccagagtc caacgctgag gcgctcgtca tcctaagcaa
     1201 ctactaccct ggctattggt gggatcacac cgacctgacc gtggcggttc aggcgcatcc
     1261 gaatgccagc gctgcccagc tcgaagcgat ccatgacgca atcgccacct ggggcgatgt
     1321 gcttgatgac tgtttccgcg ggctcattac ggtcactgat gtgacaggta ctttgaagag
     1381 cgagcagaag gctgctgaca tcgtcgtgca ttatgtgccg actgctgggg gcgttgcatt
     1441 tggaggatac gcgatatgtg gggaccatgg atgccccaat atactcgtgc ggtccgatct
     1501 gccaccgagc ctggatcgcc caccatacga gccagagtac ctcggttggg taacgctgca
     1561 tgagctcggg cacgcgcttg gcttaggaca taccaccaac ttgcttgaga gcactgatct
     1621 gatgggttat ggttggcccg atttgggcga cccagttttt tcggactgtg acatcgatgc
     1681 tcttatgttt gtcttcgcgt ggctgtttga gggcagcgaa ccccatcccc ctgctgaagg
     1741 accgtacgac tgctcactcg actgaaagcg tgtaatcacc atccacgccg ataagtggtc
     1801 ggccgatcag gcttcacact gagctaccga gtagcgatat ggtgccgctc tagttgtgtg
     1861 ccagattcga tgggtggata gaagcaactc ttccacctac aaggatcaga accgcctcca
     1921 gtgatctcgg aggcggctat ttttatcttt gtctgaaaag attatcatgc gcattattct
     1981 aagaagttaa tttcgtagta gaatggtctt aatggttcac caaactacat tccaaatgtt
     2041 catcattatt taggtcacaa acatgaaact aaaaattgta gtcctgggtg cgggcttcgg
     2101 ggggctcgag ttgagcgcga ttttgtctga agccctctct gagcgccttg atctaaccct
     2161 gatcgacaaa agcgacacct tttacttcgg ttattcaaag ctcgacgtga tgttcggtcg
     2221 taaggcaccc gattctgtca aactcgccta caagaacatt gtgaaaccag gtgtgcgctt
     2281 tcgacaggaa accatcatgg cgattgaccc cgaagcgcgc cgcgttacaa cggactccgc
     2341 ggcttatgat gccgatatcc tggtcgtagc ccttggcgca gattatgatc aagccgccac
     2401 accggggtta atagaaggtg gcaacgagta ttattctttt gccggtgcca tgcgcttacg
     2461 ggaggtgctt cccactttct cgaaaggacg cgcaatcgtc ggtgtgacct ccacaccatt
     2521 caagtgcccg ccggcgccga gcgaagccgc attattgtta catgactatc tcaccaaacg
     2581 cggtgtgcgc gataattgcg aaattagcct cgtcatgcca ttcggcatac cgatcccccc
     2641 gtcacctgcc acctccaagg ccttgctagc cgccttcgct gaacgtggga tttctttcat
     2701 acctaaccgg ctcgtgcagg ctctggaccc ggcgcgtcac gtagctattc tggacgatca
     2761 gagtgagatg ccttacgact tatttctggg gatcccgaag caccgcgtcc cggcggtggt
     2821 cgcggcaagt ggcatgactg aggatggctg ggtccccgtc aatccaaaga acctcaagac
     2881 gcgttttccc ggggtgtatg ccatcggcga tgtgacgagc gtcgggacgc caaaggcggg
     2941 agtattttcc gaaggcgctg cacgcgtcgt cgccgcttcg ctgattgcgg aacttcaggg
     3001 aagcgaacag ccctcggctt acgatggggc gggttcctgc tacatcgagt ttgggcgaga
     3061 ccttgtcggc cgcgtggatg tggatttcct ttcgggaccc tcccctacgg gtagcttcag
     3121 cgatccctcc cttgcccttg tggctgagaa ggcacaattt ggatctagtc gcaaagcgcg
     3181 ttggttcgga ttgtagtgat ggcggcttaa ctcgtggatt cagaagacat tcacaaaatt
     3241 tcctgcttga ctatttataa aagagcgcta tagtacagag gacataattt tccctgggtg
     3301 ggtggggaga tctcgcgcgt caagttttca ataagctgga atacataaaa gtcattctcc
     3361 ttgcctatga tatgccgtgg cgacagatac taaggccctt aaacggcctc gccatgccgt
     3421 cgtgcgagct agctgccagg gtagccggcc ttttgcggtg ataaaacgga gggcaaatac
     3481 cacgcatgac agacgcaacc cactcaacaa gggaacaccg cagggacctt gacctgctgc
     3541 gtgtcctgct cgtatttgga ctggtatttt ttcataccgc ccgcatcttc gacaccatat
     3601 cattaccaga gggggtcaag aaccagcccc ccagcatcgc ggcgacgatt ttcacagctt
     3661 tctttgcctt gtggggtatg ccgttaatgt tcaccgttgc tggcttcgcc atatggcact
     3721 cgctgagcaa gcgcacaggc caggccttcg tggtcgatcg gatccacagg ctggtcgttc
     3781 catttgtgtt tggagtgctg gctttggttc ctttgcaggt gtatttccac ctcaagcaag
     3841 ccgagccggc gtccaggata acctactggc aattcctcca attattcttc gatgtgcgac
     3901 tatgccctga tctggtgaat gctttcatct gccccgatcc ggcaacaggg ttgttcacta
     3961 cggctcattt atggttcctg aaggatttat ttatcttttc aatgctattg ctgcctttgt
     4021 ttctttacct aggggggcag acaggcggta atgtaatcgg gaaactgggg gactttctgg
     4081 ctcgacctgg gatgatctgg cttgtagctt tgccagtcgc cggatttgaa gccgggtttg
     4141 tcacctccac catgggagga agctggaacg agtacaccta cgcggttttg ctcatggcgg
     4201 gattcttaat cgcagccgaa ccgcgcctcg cgcaggtaat gggacgcgtg tggcggagcg
     4261 ggctggtgat tggtctggtg gtcgaggcag tctacatggt tggactttat ttgctgatga
     4321 atgtttatga tctagatccg cttcacggat acgattgggg cagcctgctc tggcggggga
     4381 taaaaagcgt tggtgcatgg gcatggatcg tcgccattat cggttacggt tcgcgaccaa
     4441 gatcaaaacc aatccctgaa ggcgtgcgaa agtccacagg aacgggtaat ctggttgccc
     4501 gcgctattgc ttatgctgga gaagcctttc ttgccttcta tatcctgcac cagaccgtca
     4561 tctttgtcat cggttattac gtggtgcagt gggcgattgg ggctcttctt aaattcattg
     4621 taatcagctt atcatcgctg gcggttaccc tgctcttata cgagctcggt gtcaggcgga
     4681 ccaaagtgac gcgctggctc ttcggcatgc gaataaaccc gcctgcccca ttggctgatg
     4741 taccgccggg aagaagtgcc ggtgaggtag cagtgtagcg gagttgacct gtttaaccgg
     4801 agatagaaat gatataattg ataatacaaa tccaatctat cggatatccc caattgctat
     4861 tcaaccgaat aaccaaggag gtccaaagtg attatcgtac gcaatgtttt tcaagccaag
     4921 tacggcaagg gtgatgaatt ggtacagctt atgaaggata gccaagccat ttggccaagc
     4981 gagcgtaaag tccgcatcat gaccgatctg agtgggccgt tcttcacggt agtcacggag
     5041 gccgactatg aaagctttag cgcctgggaa gctgatgcca agaaaacctt tggtgatgac
     5101 cgcttcgctg gcttgtttga acgcatgatg ccccttgtgg aaagtgggag tcgagagttt
     5161 tacaccctgg tagaatgaaa gctatcgtcc actaacttac cggtgaaagg tgattgtcac
     5221 gatttttgtt tgtggccgag aaatagacta cccaacaccg aaatttaggg caattgcgat
     5281 aaccaatcgg gcggtagctc aatcagatag gtctcaacct cacctgtgcc ggatcgcccc
     5341 caattgctag tgaagacaat tcgtgtgaag tcgtgattga cactggcgtg cggctcggcc
     5401 caataatcgt gctcctggtt ctcgtccacg attgagtagg tatgcaccag tctcaccaca
     5461 cgcccgcctg gcttgagttc taccacgaat atctggtcat ccatccaagt cgcggctggt
     5521 tgtgcgccgt tgtatgtcga aaccagcgcc cagccaggca tgcggctggc ccgccctgag
     5581 aaatgtaaac caagtgccga gtggctgaaa tcgatcggcc acaagggggt aacttctccg
     5641 gattccagat ccaggaggga gatattgtcg gtgtcgatat cctggaagat gagcacctct
     5701 ctttgattgg catcgagtgc taggtctgag tgaccaacaa tgcgcagtaa accccgcccg
     5761 ttttgaaggt cacgatcgta gaccattaat ccgcatgggt gggcgtcgct ccccaattgc
     5821 ccgggttcgc agtagccgtc gtaataggcg aggaagaaac ctcccaacgg gctgatgctg
     5881 acagagtcga tttccggccg ggagggcaga tcgcgcctgg cgaccacgcg atcaattttc
     5941 agatcgtaga ccagcaaggc agtagccagc cattcctggt cctcagccat gaatccccag
     6001 tagcggccat caaacgaggg gctgccctca tagcgtgtcc aaaccgccgc caggtcctgc
     6061 ccggggaagt ccactgcaaa gtcgtgcacc aaccgttcct ctgcggtcga tagacggtag
     6121 gcaagtaacc gggtttcatc tatatgatag agcaaatctg ggtcttcagc atcccagcgt
     6181 ggttcaaccg agaggggaat atgctccatg ggcttcaata tctgcgcgtc ataaacatac
     6241 caatatgcat cgatgctccg caccaaaact cggcttccgt cggcattaaa ggattgtaca
     6301 cgggagtact cgtttttcaa accctgggat gggtcttccg aggtgagatc ttcaaatcgg
     6361 tcggttaacc gcatcataca agtgccgaaa accgaatctc gaaatggcac tttggctggg
     6421 ggttcgtcca tactaggcgt ttgctgtata tcgaaaccgg tactcaggct tggaaatttg
     6481 cccttacaac tgctctcgtc tactttatcc acaagagcct cgggtacatt gttgtctctt
     6541 ggtgtctctg cgaactttgg tgtgatttct tgggctgttg cgtgttccgc aatcgaaaca
     6601 tcaccagtct gcgaagcaca tgccgtgctt gcaagggcca gtaagatgag tatccatcca
     6661 caattccagt taagtctttg tttcttggta cacatgaaat caatcagctg tgggatacag
     6721 agaattcgtc atgatcaaaa tgcaaagacg attggtcaat actatcacta tcttctcctt
     6781 tagcacattc tgatgcaatt gtcccatact atcaaggact ctagcgggca tgtgctatgt
     6841 agtgaacccc accctacatc gtctgggtgt cgaatcctag ttgaattggg aagagaattt
     6901 ctatatactg ttgatggtga tacagtcaga cgagtattcg cctcaggctg ccaaatcgag
     6961 ttattagatt ttgggaggga aaaatgcctg tcatacctag cattattgaa cgaactgttc
     7021 tgttgacact caatagaggc ccaggtccaa tgatggatct ggctggtgga atagcttttc
     7081 acgcgttatc cgttgcgatc aaactcaaga tcttcgaggc cattggagac aaaagccttc
     7141 caccaagtga aatagcccat ctgatttcca cagataccag ggcaaccggc ttgttacttg
     7201 atgcccttga agccctcggc tatgttgaag agcagcaagg aagttactct aatacaccca
     7261 tgacactcaa gtggatttta gagacttccc caaatagcct ggcttgcatg atttctggca
     7321 tggaaggagc tctagatcgc tggaagtata tggacgaaac gataagaaac ggcagtccat
     7381 caaagctagc atgggaatgg ttggatcagc atcctgggag ttgggctgat taccaggcat
     7441 tgatgattgg tatggcacat tcgtgtgctg atgaaatcgt ctcgcgggtt aaattgccac
     7501 caacagccag aaagctgatc gatcttggcg gaggccatgg gctttacagc attggattct
     7561 gccgcaagta tcctggtttg accgccacgg tattcgattg gccacaagca aaggatatag
     7621 ccaatgacac catactttca gaagggatga atgagcgcgt aaaatttgtc gtgggagatt
     7681 tgtggaaaga tgattacggt tcaggatatg atgttgcgct gttatttcag atcatccaca
     7741 tgtattctcc tgaaaagaat atccaattgc tgacgaaagt cagggaagct ttatcgccca
     7801 atggtatttt ggtgatcaac gatcaggtcg cagtagatgc accaagtccc ttagcaaaac
     7861 tgtttgcccg cctacagagt cttgaattgc ttaactcagt aaatggtcaa acctatccac
     7921 cagtggaaat tgagggatgg ctggtcgaag caggctatag caatccatcc accatgttgc
     7981 taaggaacac tccgggattt ggtgtggtcg tagcaaccaa accataaaac ctgcccttac
     8041 tctcatagcg tttattagag accgctgagc ggcatgagac agttgaggta ggtgcgcaag
     8101 tgtactttga attatcgata accatagacc gggacccgag agaagttttc gctttcttga
     8161 gggataaaga taagtttcca cagaaacctg gatcgccagt tctggtcctc gagcagacat
     8221 cccctggtcc gccaggggtc ggcacgcg
//
LOCUS       DBNGSX010000228         6597 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1195824, whole genome shotgun sequence.
ACCESSION   DBNGSX010000228 DBNGSX010000000
VERSION     DBNGSX010000228.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6597)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1195824"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1509
                     /locus_tag="AC16CB_06885"
     CDS             <1..1509
                     /locus_tag="AC16CB_06885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3342229.1"
                     /translation="FIAARSRHPMRVQMKTGVKKDGTITANEMRVLTDTGAYGCHALT
                     VTGNTGHKSMALYVGDGPYRQKPNIRFYADVVYTNHPPAGAYRGYGVPQGYWPVERHM
                     EKIARQLTLDPLEFRLKNALRAGELHPFSTAWSEGREPRPETIETCGLEECARQGKAS
                     IGWDQKYGNLEWHNPDPMRPYLKKGIGVALVMQGTAIPYLDMGGASIKMNDDGSFNLL
                     VGATDLGTGSDTVLAQMAAEVLGVPLEDMITYSSDTDFTPFDKGAYASSTTYISGAAV
                     VKAADQVAQRIKARALRMINEASSVETRQPLEVGDIRLQDRKAIAPDGRSVTLAEVAL
                     DALHHKDQEQIMGVASYVSPVSPPPFAAQFAEVTVDTETGQVRVDKLVMAVDSGIIVN
                     PVTASGQIEGGMTQALGYAVCEEMRYDEKGSARERDFRDYHIFRADEMPELETIFVET
                     FEPSHPFGVKAAAEIPMDGVAPAVGNAVLDACGANIDDNPVIPEKVWRALRT"
     gene            1580..2047
                     /gene="bfr"
                     /locus_tag="AC16CB_06890"
     CDS             1580..2047
                     /gene="bfr"
                     /locus_tag="AC16CB_06890"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006365810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HIR3342230.1"
                     /translation="MKGNDNLIMVLNELLADELTAINQYMVHSEMCADWRYEKLHEAA
                     EARAIQEMKHAEKLIGRILFLEGKPNVSNLKRIHIGSDIKEQLVNDRMAEQEAVVAYN
                     KGIQLAVDVADNGSRELLESILKDEEDHLDWLEAQLEQIEQMGIQNYLVEQTG"
     gene            2058..3404
                     /locus_tag="AC16CB_06895"
     CDS             2058..3404
                     /locus_tag="AC16CB_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013528.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA2 domain-containing protein"
                     /protein_id="HIR3342231.1"
                     /translation="MIERVYVIGHVNPDTDSIAAAVGYAWLLRERDGVDTVAARAGAI
                     NPQTTWVLKRLGLEPPLLLTDASPRFEVVTRRLDTARPDSLLREAWAITSRTRGLAPI
                     VGEDGTPFGLITGSSLFDFVAKLVGTHPRRQEMRISEILDLPCQEACDTKVPRFQAGT
                     RIRDVINRLLREEHNDFLIVDEKGLYLGVCRQRDAINPPRLRIVLVDHNEPGQALGSI
                     EEAELIEILDHHRLGNPSTHVPIKFTVDIVGSTSTLVSERMEEAGLSAPPALAGLMLA
                     GLLSDTLILTSPTTTERDRGAAERLARWAFVRNGPLAGENIHSFGEQVIGAGSGLSNR
                     DPEEVVSSDLKLYEAEDVSFAIAQVEITDLMQLDEHLTALKGALETLRQRRGVDFAML
                     MVTDVVRGSSRLLIANEPALFGDLPYPPHPDGTRLAEGVVSRKKQLLPVVLGLLES"
     gene            3469..4257
                     /locus_tag="AC16CB_06900"
     CDS             3469..4257
                     /locus_tag="AC16CB_06900"
                     /inference="COORDINATES: protein motif:HMM:NF024869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR3342232.1"
                     /translation="MIYKAIAELEHKNEAAALCTVVRSSGSTPRHAASKMLVYPDGHI
                     LGTVGGGELENRVIKEALQALKDGTPRILEYSMADPQRGDPGVCGGQLEVFVEPIMPK
                     PVLVVVGSGHVGKAVAHLAKWLGFRVVVSDDRPEFCSPEAVPDGDEFYPVPMAELPDH
                     LKITPWTYLVLTTRGVLIDVPGLPALLDTPATYIGIIGSKRRWATTRKTLLDNGFPED
                     KLSRVRSPIGLELNAETPEEIAVSIMAEIIMLRQGGDGSVMTEE"
     gene            4271..5695
                     /locus_tag="AC16CB_06905"
     CDS             4271..5695
                     /locus_tag="AC16CB_06905"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3342233.1"
                     /translation="MWHDYFSVTSVDDAIKLLAERGESARIVAGATDLILEMERGVRK
                     GIDTLIDITRVPDLDQIVLDEGNLIHLGPLVTHNHCVASPLIVEKGLPLALAAWEVGA
                     PQIRNRGTVAGNLITASPANDTITPLMALGAKVTLRSSSGVRVVPLEDFYTGVRRTVM
                     RPEEMLVDIFFPAMQPNQRGTFIKLGLRRAQAISLVNVAMLLTFQGDRVEKAAITLGA
                     VAPTIIHAPQAETFLVGRTLDENNIEEASRLAEQASRPIDDVRSSADYRSEMVRICTL
                     RGLRSLAAGTEKGDYPVQPVLLWGTEYQGAAPELSTLAQHGADTPIITKINGMIYTFT
                     AGHDKTLLRLLREEAGLVGSKEGCAEGECGACTVFLDGVAVMSCLVPAPRAHGAEIVT
                     VEGLSHDGHLHPVQQAFIEAGAVQCGYCTPGFLMSAAKLLEERPHPTRDEIQQSITGN
                     LCRCTGYYTIVNAIDRAAQLMEER"
     gene            5692..6075
                     /locus_tag="AC16CB_06910"
     CDS             5692..6075
                     /locus_tag="AC16CB_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342234.1"
                     /translation="MTKYTQYQSRGRAPARPWRVHPVWRGIGCVMMILIPVIAYAGAV
                     RLVEENLKQGWLPVPAEFARAITLPVLGSVQYLLAYLLVTVVLSLIGFGVFTALYSLI
                     YSLIGPSKYGPLDSPPIRREQRGRR"
     gene            complement(6076..>6597)
                     /locus_tag="AC16CB_06915"
     CDS             complement(6076..>6597)
                     /locus_tag="AC16CB_06915"
                     /inference="COORDINATES: protein motif:HMM:NF028436.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HIR3342235.1"
                     /translation="VRLWPHHGAGEGHFIAMLRRTDQTNRDKKKPWQPPAPSAEAARL
                     FGEFCSDHLQWNFETGSLVQIGSYLYQVPQGLPKLGHLKTIHPGLWLGTVKTGRFEPS
                     HALALALPVEQAQKVIALDFDEANTYLRGETQKISGADGWALMTLDGFPLGWGKRVKG
                     IVKNFYPKGLRWR"
BASE COUNT         1452 a         1711 c         1971 g         1463 t
ORIGIN      
        1 tttatcgctg ctcgatcgcg ccatcccatg cgcgtgcaga tgaaaactgg cgttaagaaa
       61 gacggcacca ttactgccaa cgagatgcgc gtgctcaccg atacaggcgc ctacggttgc
      121 catgccctga ccgttaccgg taacactggg cataaatcca tggcgctgta tgtgggtgat
      181 ggcccgtacc gtcaaaagcc aaatatccgt ttctatgcag acgtagtcta caccaaccat
      241 ccgcctgctg gggcatatag aggctacggg gtgcctcagg ggtattggcc agtagagcgc
      301 cacatggaga agattgcccg ccagctaacc cttgacccgc tggagttccg tttgaagaat
      361 gccctgcgtg cgggtgagct acaccccttc agcacggcct ggagcgaagg ccgcgagccg
      421 cgacctgaga ccatcgagac ctgcgggttg gaagaatgtg ctcgccaagg caaggcttcc
      481 attggctggg accagaagta tggaaatctt gaatggcaca acccggatcc tatgcgacca
      541 tatctgaaga aaggtatcgg tgtagcactg gtaatgcagg gtaccgccat tccctatcta
      601 gacatgggcg gggccagcat caagatgaac gacgatggct cattcaacct gctggtggga
      661 gccaccgatt tgggtactgg atcggatacg gtgctagcgc agatggctgc cgaagtgctg
      721 ggcgtgccgt tagaagatat gatcacctat tcttcagaca cagatttcac tccctttgat
      781 aaaggggctt atgcttccag taccacctat atctcgggcg cggctgtggt gaaggccgca
      841 gatcaagttg cccaaaggat taaggcgcgc gccctgagga tgatcaacga ggcctcaagc
      901 gttgaaacgc gccaacccct ggaggttggc gatatccgcc tgcaggatcg caaggctatc
      961 gctccagatg ggcgctctgt cacgctagcc gaggtcgctt tggacgctct ccatcacaaa
     1021 gaccaggagc agatcatggg tgtggcctcg tacgtgtcgc cggtctcgcc accccccttc
     1081 gctgcccagt ttgctgaagt caccgtggac acggagaccg gtcaggtgcg ggtggataag
     1141 ctcgtcatgg cggtcgattc cgggattatc gttaaccctg tcaccgcctc cgggcaaatt
     1201 gagggcggca tgacccaggc tttgggctat gcggtttgcg aggaaatgcg gtatgatgag
     1261 aaaggcagcg ctcgtgagcg ggattttcgg gattatcata tcttccgtgc agatgaaatg
     1321 cctgagctag agaccatctt cgtggagacg tttgagcctt cacatccatt tggggtgaag
     1381 gcggcggccg agatccccat ggacggcgtc gctccagcag tgggaaatgc agtgctggat
     1441 gcctgtggag caaatattga cgataacccg gtgatacctg aaaaagtgtg gcgggcgtta
     1501 aggacataaa tataaatacg actcgggtgc tttgatcggc gcgcgggtcg aagatttgta
     1561 aaaaagagag ggcagaacaa tgaagggcaa cgataatctc atcatggtct tgaatgaact
     1621 attagctgat gaattgacgg cgatcaacca gtacatggtt cattccgaaa tgtgcgctga
     1681 ttggcgctat gaaaaactgc atgaggctgc cgaagcgcgc gctatccagg agatgaagca
     1741 cgcagaaaaa ttgattgggc gcatcttgtt cctggagggg aagccaaatg tgagcaatct
     1801 gaagcgcatc cacattggaa gcgatatcaa ggagcagctc gtcaacgatc ggatggctga
     1861 gcaagaggcg gttgtcgcgt ataacaaagg cattcagctg gcggtcgatg tggctgacaa
     1921 tggcagccga gagttgcttg aatcaatctt gaaagatgaa gaggatcacc tcgattggtt
     1981 ggaagcccag cttgagcaga ttgagcaaat gggcatccag aattaccttg tagagcaaac
     2041 cggttaagcc aaaggatatg atcgagcgcg tatacgtcat tgggcacgta aatccagaca
     2101 cagattccat tgctgcagca gtgggatacg cttggctctt gcgcgagcga gatggagtgg
     2161 ataccgtcgc cgctcgcgca ggggcgatta acccccagac cacctgggtg ctcaagcgcc
     2221 tggggctgga accgcccttg ctgcttaccg atgcatcgcc gcgctttgaa gtggtcactc
     2281 gccgtttgga tactgccaga ccggatagcc tattgcgtga agcctgggcg atcaccagcc
     2341 gaactcgcgg ccttgctccg attgtgggcg aagacggcac cccctttgga ttgatcaccg
     2401 gcagcagctt gttcgatttt gtggccaagc tggtcggtac ccatccccgt cgccaggaaa
     2461 tgcgcatcag cgagatcctt gatctgcctt gccaggaagc ctgcgatacc aaggtgccgc
     2521 gctttcaggc gggtacgcgc attcgggatg tgatcaaccg attgctgcgc gaagagcata
     2581 acgatttcct gattgtagac gagaagggat tgtatctggg tgtttgccgg cagcgcgacg
     2641 cgatcaatcc gccgcgcctg cgcattgtcc tggtcgatca taatgaaccc gggcaagcac
     2701 ttggttctat tgaggaagct gaactgattg agatcctgga tcatcaccgt cttggcaacc
     2761 cgagcacgca cgttcccatc aagttcactg tggatatcgt aggaagcacg agtaccctgg
     2821 tatctgagcg tatggaagag gcggggttga gtgcaccacc tgccctggct ggtttgatgc
     2881 tggcagggct gctctcggat acgctaattc tcacctcgcc taccacaacc gaacgcgata
     2941 ggggtgctgc cgagcggctg gctcgctggg cttttgtacg gaatggcccg ctagctggtg
     3001 agaatattca ttcctttggg gagcaggtga taggagctgg tagtgggctg tctaatcgcg
     3061 atccggagga ggtcgttagt tcggatctca agctatacga agccgaagat gtgtcctttg
     3121 ccattgctca ggttgagatc acagacttaa tgcagcttga cgaacatctg acagcactca
     3181 aaggtgccct ggagaccttg cgccagcggc gtggtgtgga ttttgccatg ctgatggtca
     3241 ccgatgtggt gcgtggctcc agtcgcctgc tgattgccaa cgaaccagcg ctttttggcg
     3301 atttacccta tcccccgcat ccagatggca cgcgtctggc agagggtgta gtctcgcgga
     3361 aaaagcaatt gttgccagta gtattagggc ttttagaaag ttgagaatat gcgatgatat
     3421 tcgaaacctt caaaagcaga ttttctgtgt gaaaattgcg ttcaaattat gatctataaa
     3481 gccattgccg aattagaaca caagaacgaa gcggcggcat tatgcacggt ggtgcgaagt
     3541 agcggatcta ccccgcgcca tgctgccagc aagatgctgg tctatcctga tgggcacata
     3601 ctcggcactg ttggtggcgg tgagctggag aaccgggtga ttaaagaggc tcttcaagct
     3661 ctgaaagatg ggacaccccg catattagaa tatagcatgg ctgacccgca gcgcggcgat
     3721 cctggagtgt gcgggggtca gctggaagtt tttgtggagc cgatcatgcc gaaaccagta
     3781 ttggtagtgg taggcagcgg tcacgtgggc aaagctgtgg cgcacttagc caaatggctg
     3841 ggtttccggg tggtggtcag cgatgatcgc ccggagtttt gcagcccgga ggccgtgccc
     3901 gatggggatg aattctaccc ggtgccaatg gctgaattgc cagatcactt gaagatcacc
     3961 ccctggacct atctggtgct gactactcgc ggggtgctta ttgatgtgcc cggattacca
     4021 gccctgctgg acacaccagc gacctatatt gggattatcg gctcgaaacg ccgctgggct
     4081 accacgcgta agacgctgct cgataatggt ttcccagagg ataaactgag ccgggtgcga
     4141 tcgccgatcg gtttggagct gaacgctgag acgccagaag agatcgctgt cagcatcatg
     4201 gcggagatca ttatgctccg ccagggcgga gacggaagcg tgatgaccga ggaataaacc
     4261 tggagattct atgtggcatg attatttcag tgtcaccagt gtggatgatg cgataaaact
     4321 cctggcggag cgtggcgaga gcgcacgcat tgtagctggc gccaccgacc tgatcctgga
     4381 gatggaacgc ggcgtccgca aaggcattga taccttaatc gatattaccc gcgtgccgga
     4441 tctcgaccag atcgtcctgg atgaaggcaa cctgatccac ttgggtccat tagttaccca
     4501 caaccattgt gttgcttctc cattgatcgt cgagaaggga cttccattgg cgctggctgc
     4561 ctgggaagta ggcgctccgc agattcgcaa ccgcggcaca gtggcgggca atctaatcac
     4621 cgcctcacca gcaaacgata ccattactcc cttgatggct ttgggcgcta aggtaacctt
     4681 gcgatccagc tctggagtac gcgtagtccc cctggaagat ttttacactg gcgtgcggcg
     4741 cacggtgatg cgccctgaag agatgctggt ggatatattc ttcccggcga tgcaacccaa
     4801 ccagcgaggc acattcatca aattggggct gcggcgtgcc caggcaatct ccctggtgaa
     4861 tgtagcgatg ctcctgactt tccaaggcga ccgcgtggag aaggctgcca ttacacttgg
     4921 ggcggtggct cccaccatta tccatgcacc acaggctgag acattcctgg tgggtcgcac
     4981 cttagatgaa aacaacattg aagaagcgtc tcgccttgca gagcaggctt cccgcccaat
     5041 tgacgatgtg cgcagctccg ccgattaccg cagcgagatg gtgcgtatct gcacattacg
     5101 cggattgcgc tctttggccg ccggtacgga gaagggtgat tacccggtgc agcctgtcct
     5161 cttgtggggc acggagtatc aaggggcagc tcctgaatta tctaccttgg cgcagcatgg
     5221 cgcagatact ccaatcatca ccaaaatcaa cgggatgata tacaccttta cggccggaca
     5281 tgataaaacc ttgttacgcc ttttgcgcga ggaagctggc ctggtaggct ccaaggaggg
     5341 ttgcgccgaa ggggagtgtg gggcgtgtac ggtcttttta gacggggtag ccgtgatgag
     5401 ctgcctggtg ccagcgccgc gcgcacatgg ggctgagatt gtgactgtag aaggcctgtc
     5461 tcacgatgga catctgcacc ctgttcagca ggcttttatc gaggcgggtg ctgtgcagtg
     5521 cggctactgc accccagggt tcctgatgtc ggctgcaaag ctgctggagg agaggcctca
     5581 ccccactcgc gatgagatcc agcaatccat aaccggcaac ctgtgccgct gcacaggata
     5641 ttatacgatc gtgaatgcga tcgacagggc tgctcagcta atggaggaac gatgaccaaa
     5701 tacactcagt accaatcccg cggccgcgca cctgcacgtc cctggagggt gcacccggtc
     5761 tggcgcggca tcggctgcgt gatgatgatc ttaatccctg tgatcgctta cgcgggcgca
     5821 gttcggcttg ttgaggagaa cctgaagcag ggttggttgc ctgtgcctgc cgagtttgcg
     5881 cgtgcgatta ccctccctgt gctgggtagc gtccaatatt tgctggctta cctgctggtg
     5941 actgtcgtct tgagcctgat cgggtttggc gtttttactg cgctttattc ccttatctat
     6001 tccctcatcg gtccgtcgaa gtatggacct ttagactcac ccccgataag gcgagagcaa
     6061 agagggcgca ggtgattaac gccagcgtag gccttttggg tagaagttct ttacgatacc
     6121 tttgacccgc ttgccccaac cgagcgggaa gccatccaag gtcatcagcg cccatccatc
     6181 cgccccgcta attttctgtg tttccccgcg caaataagtg ttcgcctcgt cgaagtcgag
     6241 ggcgatcacc ttctgcgcct gttcgacagg cagggccagc gccagggcgt gcgagggctc
     6301 gaagcgcccg gttttgaccg tgcccaacca taatccaggg tggatggttt tcagatggcc
     6361 aagttttggc aacccttggg ggacctggta caggtaagag ccaatctgga ccagggagcc
     6421 ggtctcgaaa ttccactgca agtgatcgga gcagaattcg ccaaacaggc gagcggcttc
     6481 tgcagatggg gctggcggtt gccagggctt ctttttatct cggttggttt gatcagtcct
     6541 acgaagcatg gcaataaaat gtccttctcc tgcgccgtga tggggccata agcgcac
//
LOCUS       DBNGSX010000229         3230 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1403356, whole genome shotgun sequence.
ACCESSION   DBNGSX010000229 DBNGSX010000000
VERSION     DBNGSX010000229.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1403356"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="AC16CB_06920"
     CDS             <1..577
                     /locus_tag="AC16CB_06920"
                     /inference="COORDINATES: protein motif:HMM:NF014647.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3342236.1"
                     /translation="TLAIEHFSVIEGTTWSGAQDRAGKRIAEKYPNVEYVYRENVSPD
                     QSVPFAEELIAQGADIVVGNAEFMGMPLIDIADKYPDVYFGSIIASDLSTKRNFIRLF
                     PRQYQALYLEGLIAGALTQTGNIGIVSAFPNVQVIRRTAGFYLGVMDAAELLNKDINV
                     YVKYAGDWYLPTEEADIANTLVDQYKVDVIT"
     gene            644..1129
                     /locus_tag="AC16CB_06925"
     CDS             644..1129
                     /locus_tag="AC16CB_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342237.1"
                     /translation="MDTVGEYGWSNTDTVAVSFDTRWEVLYERIVKSYLAGEQNPETV
                     LYLGMSHKMTLADGTEEYTIDLMNDGKVGVEAISPKALPLIPQEIIDLVDQRRSQMID
                     GVWDPFFEHEFVSNGTGLELEGLPIPAAGTVVKPAGEMPTDDWLLSKFNFDMQGMIIL
                     D"
     gene            1288..2835
                     /locus_tag="AC16CB_06930"
     CDS             1288..2835
                     /locus_tag="AC16CB_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3342238.1"
                     /translation="MVLGGAKRGEMVLEARGITKRFPGVLANDQIDFEIKAGEIHAIL
                     GENGAGKTTLMNILFGLLHPEEGEIHIRGERVKFKSPLDAINMGIGMVHQHRKLVSAH
                     SAIENIILGHPSAGFILNVKKAEEEVQELCDRYGFKVDLRAKIWQLSEGEKQAVEILK
                     ALYRGAEILILDEPNSALTPQETEKLLESIEAMARDDLAIVPFITHKLPIVLGISHRV
                     TVLRRGKVVACLETSRATEQSLAMQMVGREVIFRIEKTTVERGKPILQVENLSALSDK
                     GFQALSDVSFTICEGEIFGLAGISGNGQQELVEVLAGIRNVHKGKVTLDGQDITHASS
                     LDRWRRGIGYIPADRINVGSIPDFSLVENTAMNYFFDDRYSQHGIVDYKNMRKLTEEV
                     IAEYGVAAPGPDTKAKNLSGGNLQKLILARVLSRNPRLVIADLPCQGLDVGATEFVQN
                     KLMEAKKGKAGVLLISEDLDEILSMSDWVAVIYEGKFMGILPTGQAKKENVGAMMAGL
                     SLKDNVT"
     gene            2832..>3230
                     /locus_tag="AC16CB_06935"
     CDS             2832..>3230
                     /locus_tag="AC16CB_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3342239.1"
                     /translation="MKIGRNEFKMEKRATLSGWEAARISILAIIFALTLLSLLFILAD
                     VNPLIAYREIFSYAFFNPFGLPLTINRSIFLLLCTFAFIIPYKAGLWNIGMTGQLFAG
                     SICAFAVVVALGGKEFSASPSPWLLIPLMLI"
BASE COUNT          859 a          743 c          855 g          773 t
ORIGIN      
        1 taccttggcc atcgagcact tcagcgtcat cgaagggaca acctggtctg gagcccagga
       61 tcgcgccgga aagcgtatcg ctgaaaaata tcccaatgtc gagtatgtat acagagagaa
      121 tgtatcaccc gatcaatccg ttccttttgc ggaggagttg atcgcccagg gagctgacat
      181 cgtcgtgggc aacgctgaat tcatggggat gcccctgata gacattgcgg ataaataccc
      241 ggacgtctat tttggctcta tcatcgccag cgatctaagt acgaagagga acttcatccg
      301 cctcttcccc cgccaatatc aggcgcttta cttggaaggg cttatcgccg gggcattgac
      361 tcagacggga aatatcggca tcgtatcagc attccccaac gttcaggtca tccggcgaac
      421 agccggcttt tacctgggag tgatggatgc agctgagttg ctcaataagg atatcaacgt
      481 ctacgtcaaa tatgccggtg actggtacct gcccacagaa gaggcggata ttgccaacac
      541 cctggttgac cagtacaagg tcgatgttat cacctaacaa accgattccg gatctcccct
      601 tgatgttgca caaaataagg gtatctggtt cgtagggaag gatatggaca cggttggaga
      661 atatggctgg tcaaatacag acaccgtagc cgtctccttc gacacacgtt gggaagtcct
      721 ctatgagagg atcgtcaaga gctatctagc cggtgagcaa aatccagaga ccgtccttta
      781 cctgggtatg tcccataaaa tgacgctagc agatggcacg gaagaataca ccattgacct
      841 gatgaacgac ggcaaagtgg gcgtcgaggc gatcagtcca aaggcgctgc ccttgatccc
      901 gcaagagatc attgatctcg tcgaccagag aaggagccag atgatcgatg gagtttggga
      961 tccgttcttc gaacacgaat tcgtgagcaa tggaactggc ctggaactgg aggggctgcc
     1021 cattcctgcc gcgggaaccg tggtcaagcc agccggtgaa atgccaacag atgattggct
     1081 gctttccaaa ttcaactttg acatgcaagg catgatcatc ctggattaac aaggagacga
     1141 tcagctctgc ttattctgat tgacggataa ctaccattac atccaagagg cttgaggctc
     1201 ccaataagcc gggagattcc cgaatcggtt gggagcctca agccagacat tatcttgtat
     1261 cctaattaga gaagcgagaa gatgattatg gtcctaggag gagcaaaacg cggagagatg
     1321 gtcttagagg ctcgcggaat cacaaagaga tttccggggg tcttagcgaa tgaccagatc
     1381 gattttgaga tcaaagccgg ggagattcat gccatccttg gagaaaatgg cgccggtaag
     1441 accaccttaa tgaatatcct gtttgggctc ttacatccag aagaaggtga gattcatatc
     1501 cgcggggaaa gggttaagtt taaatctcct ctggatgcga tcaatatggg cattgggatg
     1561 gtccaccaac accgcaaact ggtatcagct cactctgcaa tagagaatat catccttggg
     1621 catccgagtg ctggttttat tctcaatgta aagaaagcag aagaggaggt tcaggaactc
     1681 tgcgaccgat acggttttaa agtggacttg agagccaaaa tctggcaact ttccgaaggt
     1741 gaaaagcaag ccgtcgaaat tttaaaggcg ctttaccgcg gggctgaaat cctgatcctg
     1801 gatgaaccca attctgcact gactccccag gaaacggaga aactcctgga atccatcgag
     1861 gcgatggcca gggatgacct cgccatcgtc ccattcatta cacacaaact acccattgtc
     1921 ttggggatca gtcacagggt gacagtctta aggcgcggca aagtggtagc ctgtttagaa
     1981 acctcgcgcg caaccgagca aagcctggct atgcaaatgg taggccgcga agtcattttc
     2041 aggattgaaa agacgacggt tgagaggggt aagcccatac tccaggtgga aaacctatct
     2101 gccttgagcg ataaaggatt tcaggcatta agtgatgtgt catttaccat atgtgaaggc
     2161 gagatcttcg ggttagctgg aatatccgga aacggacagc aggaattagt cgaggtgctt
     2221 gccggaatac gcaacgtaca taaaggtaaa gtgactctgg atgggcaaga catcacccat
     2281 gcctccagcc tggatagatg gcgaaggggc attggctaca tccccgctga tcgcatcaac
     2341 gtgggctcta taccagattt ctctctggtt gaaaatacag ccatgaatta tttcttcgat
     2401 gatcgctatt cccaacatgg catcgtggat tataagaata tgcgcaagct taccgaagag
     2461 gttattgccg aatatggagt agcagcaccc ggacctgata ctaaggcaaa aaatctttcg
     2521 ggaggcaatc ttcagaagct cattctggcg cgcgtattat cgcgcaatcc gagacttgtc
     2581 atcgcagact tgccctgcca ggggctggat gttggagcta cggagttcgt ccagaacaag
     2641 ctcatggagg cgaagaaagg caaagctggc gtcttgctca tttccgaaga cctggacgag
     2701 atattatcga tgagcgattg ggtagcggtg atttacgagg gcaagtttat gggcatcctt
     2761 ccgacagggc aagcgaagaa ggagaatgtg ggtgcgatga tggctggttt atcgctgaag
     2821 gataacgtga catgaagatc ggtcgcaacg aatttaagat ggaaaagagg gcaaccttat
     2881 ctggttggga ggctgcccgc atctcgatcc tggcgataat cttcgcgttg acgcttctaa
     2941 gcctgctatt tatcctggca gacgtcaatc cactcatagc atatcgggaa attttttctt
     3001 atgctttttt taatccattt ggactacctt tgaccatcaa cagatctatt ttcttgctgt
     3061 tatgtacatt tgcattcatc atcccttaca aagctggtct gtggaatatc ggcatgacag
     3121 ggcagctttt tgccgggtca atatgtgcat ttgccgttgt agttgccttg ggtggaaagg
     3181 agtttagcgc gagtccatcc ccatggttgc tcattcctct aatgttaatt
//
LOCUS       DBNGSX010000230         6215 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_988699, whole genome shotgun sequence.
ACCESSION   DBNGSX010000230 DBNGSX010000000
VERSION     DBNGSX010000230.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_988699"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1282)
                     /locus_tag="AC16CB_06940"
     CDS             complement(<1..1282)
                     /locus_tag="AC16CB_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342240.1"
                     /translation="MYNQIANPKKNAHTRPRDILNRALLRLVALSLAIAYGGGLWLHL
                     LHEFEGGHELMPVHPVLHWLRDASLALPLITLAAWLALRLAASFTRAKGWQAARLRSR
                     LVEVAALTLLTSGFVMAGNPIHGLLFGASHQHETSFTSHLLRDGLLSLLMNLFIVWVF
                     VFLWPATAPRPERRRPARKLMLGGASLAAVVIVAAMLLSPLSALSGLPALLPQPANAA
                     VCARTITADIVALDQPFYYNRLGAIQANGMMYALARDVVVAPNLNPATDPATGLPFVF
                     PDVTAFAGKTIPEFDAAGRNRADLAGQVSLRADKRPRPIVLRVNEGDCLEITFTNLLD
                     PVEFIFPLPAGFPTGQILGLGVDIPPQVNDQPLTRQASIHIDGLYLVDPDGESGPLPG
                     IASDGSNVGQNTPSSLVAPGGSTTYTYHAEDEATH"
     gene            complement(1293..1679)
                     /locus_tag="AC16CB_06945"
     CDS             complement(1293..1679)
                     /locus_tag="AC16CB_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342241.1"
                     /translation="MLHTQIWYIMVGMEGIRVCLVGNTLFADNVAQMLRGCEVVSQVD
                     HFPSVPEAMHYIDSQPPNVLVLADVDATILVGDTPFLPICPDIPVICTDYQSNLLKLI
                     NTTHVSARLPDLIKAVTTLKAAQAQR"
     gene            complement(1738..2433)
                     /locus_tag="AC16CB_06950"
     CDS             complement(1738..2433)
                     /locus_tag="AC16CB_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3342242.1"
                     /translation="MPKARICIADDHELFREGLVQLINRQADMEVVGTASDGLEMLTL
                     ARELKPDLILMDIHMPISDGVEATHLIREFDQDITIVILTALDSDEKLVEAIRAGAEG
                     YMLKNTSSEGFLRAVRGALDGETTLPRHLTGRLVKEYAQLAKQPRQPAGSYDSPTLTS
                     REIQVLELIARGASNQQIAEQLAISLYTTKSHVRNLLGKLGVQNRWQAADMAKDMGLL
                     RTHKRGMERGLED"
     gene            complement(2426..4327)
                     /locus_tag="AC16CB_06955"
     CDS             complement(2426..4327)
                     /locus_tag="AC16CB_06955"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HIR3342243.1"
                     /translation="MQILRTVHSKVIALVIIFFLLVLSSFLATYLTVQTHFWDENTIE
                     LVHKEISIVQELTWLALSGGSPAEIDAEILAYDQDLEAVRAAVRQLEKSGSIQGTQDI
                     HLHIDALKQAWRKYQISLRSLTALPLQDPGRAQAEQDLRAETLKVFTQIEQLSHAVEL
                     YSSSRHILLVNIQVAFVIIAGAFTLLGYLLIRQRVLRPLSALTNAAQQIEKGDLNTRL
                     DIPGEDEIGQLSRSFDQMREAVLGYQTGLETSIKERTAEIVTAFEFSQEIVSQLNLDQ
                     LIQSVVDRSRTLMRADGASLCLLTPDGGSVELASTTNHKQAVKKLVQPIGDNLPDVVV
                     ASNRTISSQVSSFGCSFLQHIPDHQCLSAPLHVGEQVIGAICVVRSKDQPFEEEESRA
                     FSLLANSAAIAILNARYAEQARQLAQRAAALSERERLASELHDNLAQTLNLVNFKIGQ
                     IQAMLSDSMSETAQTEIKQVQTNVESAIEEVRLVVSEMASPANAYEEELLPQLERSVE
                     EFEQATGIVVGVTGVESAHTRLTALAQKQLLMIISEALTNIQRHAEAENVSLHFCADQ
                     DSLHVTIEDDGKGFQPEMIKGNHHLGLRIMRTRAERSGGTVDIVSSPGKGTRVNACFP
                     LGEGEQTDA"
     gene            complement(4851..5909)
                     /locus_tag="AC16CB_06960"
     CDS             complement(4851..5909)
                     /locus_tag="AC16CB_06960"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIR3342244.1"
                     /translation="MEHVRVGVIGTGRMGQRHCRVFSNLRRVQLVGVYDANPVVGYKV
                     AQQYDAPFFDSIDELLKQVDAVSLATPTFFHHEQAMLCISKGLHVLVEKPITRTLDQA
                     EALVKAAEESGLVVQIGHIERFNPAYIELKNILEEYQPLSITLQRLSPYQGSNKDVDV
                     VMDLMIHDANLMLDLLKKNPTKMSAYGLTAYSGTIDHAIAQLIFEGGPMLTMTSSRLT
                     EEKIRKIDVTCREAYIECDLLNKSISVHRSTFGEYLNSNRRGVKYRQEGIIERINIPS
                     FEPLFLQLQHFVDCILEEKTPYVTAQDGYDALKLTLYIRDCICEHLINMDRRKTPRVD
                     TPSIMIEPSVNVSSPRPN"
BASE COUNT         1336 a         1659 c         1741 g         1479 t
ORIGIN      
        1 ggtgggtggc ctcatcctcg gcgtggtagg tgtaggtggt gctgcccccc ggagcgacca
       61 ggctgctggg ggtgttctgg cccacgttgg agccgtcact agctattcca ggcagcgggc
      121 cactctcccc atcgggatcg accaggtaca ggccgtcgat atggatggag gcctggcggg
      181 tgagcggctg gtcattcacc tgtgggggaa tatccacgcc caggcccagg atctgcccgg
      241 tggggaagcc agcgggcaag ggaaagatga actcgaccgg gtcgagcagg ttggtgaagg
      301 tgatctccag gcagtcgccc tcgttcacgc gcagcacgat cggccgcggg cgcttgtcgg
      361 cgcgcaggga gacctgaccg gccagatcag ctctgtttct tccagcagca tcaaattcgg
      421 gaatggtctt acctgcaaaa gcagtaacat ctggaaacac aaaaggaaga cccgtcgctg
      481 gatcggtagc tggattaagg ttgggcgcga ccaccacgtc ccgcgccagg gcatacatca
      541 tgccgttggc ctggatggcg cccagccggt tgtagtagaa gggctggtcc agcgccacga
      601 tgtcggcggt gatggtacgg gcgcaaaccg cagcgttggc cggctggggg agcagcgccg
      661 gcagaccgct cagggcgctc agcgggctga gcagcatggc agccactatc accacggccg
      721 ccagcgaggc gccgcccagc atgagcttcc tggcaggtcg ccgccgctca gggcgcggcg
      781 cagtcgccgg ccacaagaag acaaacaccc acacgatgaa caggttcatc agcagcgaca
      841 gcaggccgtc gcgcagcagg tgggaggtga aagacgtctc gtgctggtgg ctggcaccaa
      901 acagaaggcc gtggatgggg ttgcccgcca tcacaaagcc gctggtcagc agcgtcagcg
      961 cggcgacctc caccagcctg ctgcgcaggc gcgcagcctg ccagccctta gcgcgggtga
     1021 acgacgccgc aaggcgcagc gccagccagg ccgccagggt gatcagcggc agggccaggc
     1081 ttgcatcgcg cagccagtgc aggaccggat ggaccggcat gagctcgtga ccgccttcaa
     1141 actcgtgcag caggtgcagc cacaaaccgc ctccataggc gatggcaagc gataatgcca
     1201 ccaacctcag gagtgctcgg ttcaggatgt cccttggacg agtgtgtgca tttttcttgg
     1261 ggtttgcgat ttggttatac atttcctctc ctttatcttt gcgcctgggc ggctttgagt
     1321 gttgtcaccg ctttgatcag gtctgggagt ctagcagata cgtgtgtggt gttgattagt
     1381 tttaaaagat tgctttgata gtcggtgcaa atgacgggta tgtccgggca gatgggcagg
     1441 aagggggtgt cgcctaccaa gatggtggcg tcgacatcgg ccagcaccag cacgttgggg
     1501 ggctggctgt cgatgtaatg cattgcctcg ggtaccgaag ggaaatggtc cacctggcta
     1561 accacctcac acccgcgcag catttgagcc acattgtcgg caaaaagcgt atttccaacc
     1621 aaacacaccc ggattccttc cataccaacc ataatgtacc aaatttgggt atgaagcata
     1681 aatccctccg ctggcgaaaa cggagggatt ttggaggtat atttaatagt taaaggacta
     1741 gtcctctaag ccccgctcca tacctcgttt gtgggtgcgc agcaggccca tatctttggc
     1801 catatcggcg gcctgccagc ggttttgtac ccccagcttg cccaacaagt tgcgcacgtg
     1861 gcttttggtc gtgtaaaggc tgatggcgag ctgctcggcg atttgctggt tgctggcccc
     1921 gcgggcgatc aactccagca cctggatctc tcgcgaggta agggtgggcg agtcgtagga
     1981 acccgcgggc tgccggggtt gtttggcgag ctgggcatat tccttgacca ggcgcccggt
     2041 gagatggcgc ggcagggtgg tctccccgtc cagggcacct cgtaccgccc gcagaaaacc
     2101 ttccgagctg gtatttttga gcatgtagcc ttctgcgcca gccctgatgg cctccaccaa
     2161 cttctcatcg ctgtccaggg cggtcaaaat gacgatggtg atatcctggt cgaattcgcg
     2221 gatcaggtgg gtggcctcta ccccgtcgct gatgggcatg tggatgtcca tgaggatcag
     2281 atcgggcttt agctcgcggg ccagggtgag catttccaac ccatcgctgg ctgtgcccac
     2341 cacttccata tctgcctgcc ggttgatcag ctgtaccagc ccttctctga agagctcgtg
     2401 atcgtcggcg atacaaattc ttgctttagg catcagtctg ttccccttca cccagcggga
     2461 aacaggcgtt cacccgtgta cctttgccgg gtgatgacac gatgtcgacc gttcccccgc
     2521 tccgctcagc ccgggtgcgc atgatgcgca gtcccaggtg gtggttgcct ttaatcatct
     2581 cgggttggaa acccttgccg tcatcttcga tggtcacgtg caggctatcc tggtcggcgc
     2641 agaagtggag cgaaacattc tccgcctcgg cgtggcgctg gatattggtg agggcttcgc
     2701 tgatgatcat caacagctgt ttctgtgcca gggccgttaa acgggtgtgc gctgactcca
     2761 ctcctgtcac acccaccacg ataccggtcg cctgttcgaa ttcctcgaca ctgcgttcca
     2821 gctgaggtaa taactcttct tcatatgcat tggctgggct agccatttcg ctcacaacca
     2881 gccgcacttc ttcgatggcg ctctctacgt tggtttgcac ctgtttaatc tcggtttggg
     2941 cagtctcgct catcgaatcg cttaacatgg cctggatttg gccgatcttg aagttgacca
     3001 aattcagggt ctgggccagg ttatcgtgca gctcggaggc caggcgctcg cgttccgaca
     3061 gtgccgcggc cctctgggcc agctggcgag cctgttcggc atagcgagcg ttcaatatag
     3121 cgatagctgc tgagttggcc agcagggaaa aagcgcgcga ttcttcttcc tcgaatggct
     3181 ggtctttgct gcggacaacg caaatggcgc caatcacttg ctctccaacg tgcaacggtg
     3241 cagacaggca ttggtggtcg ggaatgtgct gtaagaagga gcaaccgaag ctggagacct
     3301 gggaggagat agtgcggttg ctcgccacca ccacatcggg gagattgtcc ccaatgggtt
     3361 ggaccaactt cttcactgcc tgcttgtgat tggtggtgga agccagttca accgagccgc
     3421 catctggggt cagcaggcac aacgaggcac catctgctcg catcaaggtg cgtgatctgt
     3481 caacaacaga ttggatcaat tggtctaagt taagctggct gacaatctcc tggctgaact
     3541 caaaggccgt gacaatttca gcggtgcgtt ctttgatgct tgtttccagg cctgtctggt
     3601 aacctagtac cgcctcccgc atctggtcaa atgaacgcga cagctggcct atttcatctt
     3661 ctccagggat gtctaagcgc gtattcagat cacctttctc gatctgttgc gcagcattgg
     3721 tcagggcgga cagcggtctg agaacgcgct ggcggatcag caggtatccc agcaatgtga
     3781 acgcgcctgc gatgatcacg aaggcgactt gaatgttcac cagcaggata tgcctggaac
     3841 tcgaatatag ttccactgcg tgagacagtt gctcgatttg agtgaacact ttcagggtct
     3901 ctgccctcaa atcctgttct gcctgggctc tgccgggatc ttgcaagggg agcgcggtga
     3961 gcgatctcaa cgaaatctgg tactttctcc aggcctgttt gagtgcatcg atatggagat
     4021 ggatgtcctg cgtcccttga atcgagccac tcttttccag ctggcgtact gcagcccgca
     4081 cggcttcaag gtcctggtca taggctaata tttccgcatc gatctccgca gggcttccac
     4141 cgcttagagc cagccaggtt aattcttgga cgatggagat ctctttatgc accagctcga
     4201 tggtgttttc atcccaaaaa tgagtctgaa cagtcaggta tgtggcgaga aatgaagaca
     4261 agaccagcag gaaaaatatg atgaccagcg caatgacttt gctgtgcacc gttctcaata
     4321 tttgcatcta cttcccccag taagagtggt ttcgcatggt attacagtta atctgatgtg
     4381 atattttctt gtttttatta attcaagtta ggcaccagcc gagtgctatc aaccatttag
     4441 aatccaataa aatcgctcat atggggatgg ctttgaaatc cgttttggat cgatgcttga
     4501 ttgaactacc ccgacttaac aatagcttaa tacacttgcc ctgattttac cacccttctt
     4561 atctgatttc aagcgatttt caatattata agtacattgt aagctttaat ttaattagga
     4621 cttacgcagt tgaagaggga agagggggag tttgggcggg tcatgacccg cccaataaca
     4681 ccccaaatac acggttatct caggctcagg ttattaatga agagaaatac cagcctggcc
     4741 attcttgcta tcaagaagaa gacatcgtat ctattggaca ttaatccatg gcgtaaaaaa
     4801 aacagagcct gggttcccag gctctgtgat tcgtctgatg gcatgctgca ctaattgggt
     4861 ctcggcgagc tcacattaac cgagggctcg atcataatcg agggagtgtc gacgcggggt
     4921 gtttttctac gatccatgtt gatcaaatgc tcgcagatac aatctcggat gtataaggtc
     4981 agcttcaagg catcatatcc gtcctgggcg gtcacatagg gggttttctc ctccaggatg
     5041 caatccacaa agtgctgcaa ttgcaggaag agaggctcaa acgaagggat gttgatccgt
     5101 tcaatgatcc cctcctggcg atacttcacc cctctacggt tggaattcag gtattctcca
     5161 aatgtgctgc ggtggacaga tatgctcttg ttgagcagat cgcactcgat atatgcttcc
     5221 cggcaggtga catcgatttt gcggatcttc tcctcggtga ggcgcgagga tgtcatcgtt
     5281 agcataggcc cgccttcaaa aatcaattgg gcaatggcgt ggtcgatggt gccgctgtag
     5341 gctgtcaagc cataagcgct cattttcgtc ggatttttct tcagcagatc cagcatcagg
     5401 ttggcgtcat ggatcatcag atccatcacg acgtccacat ccttgttgct tccctggtat
     5461 ggactcaggc gttgcaaggt gatcgataga ggctggtatt cctctaaaat attcttgagt
     5521 tcaatatacg ccgggttgaa gcgctcgatg tgacctatct gaacaaccag accgctttct
     5581 tcggctgcct ttactaaggc ttctgcctgg tctaaggttc ttgtgattgg cttttccacc
     5641 aagacgtgca acccttttga gatacatagc atggcctgtt catggtggaa gaaggtgggc
     5701 gtcgccaggc tgactgcgtc cacctgtttt agcaattcgt cgatgctatc aaaaaagggt
     5761 gcatcgtatt gttgagccac cttataaccc actactgggt tggcatcata tacgccgacc
     5821 aattgcactc tccgcaaatt cgagaaaacg cggcagtgtc tttgacccat gcgccctgtg
     5881 ccaattactc ctactcgaac atgttccata atgccctttc ctttcatcta caaaagaaga
     5941 ggcttgaatg accaccccgc ctttggtaac ccaatggacc gccaggcact tatggttgtg
     6001 tgcaagagac aaggcaggtc ggcacaactt gcaaggtcaa ccaggaaatc cggcggtcat
     6061 ggaatacgtt gcttaagact atccccttag acctcgccac tgtgcaagcc tatatgattt
     6121 tcgacttacc attttggaga taaaaggggc gtgctagata aagggataat gaccttggaa
     6181 aaatctacat ctatctccga ttaataaaac gaatc
//
LOCUS       DBNGSX010000231         8750 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_849594, whole genome shotgun sequence.
ACCESSION   DBNGSX010000231 DBNGSX010000000
VERSION     DBNGSX010000231.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8750)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8750)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8750
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_849594"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..164
                     /locus_tag="AC16CB_06965"
     CDS             <1..164
                     /locus_tag="AC16CB_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR3342245.1"
                     /translation="VARFRKELGREMKVIATGGLAEVIARETAVLEIIAPWLTLDGLR
                     IIWELNQSK"
     gene            161..781
                     /locus_tag="AC16CB_06970"
     CDS             161..781
                     /locus_tag="AC16CB_06970"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR3342246.1"
                     /translation="MTKTVILPADDPVSTRRALEILGQGGLVAFPTDTVYGVGALVSK
                     AQAIAQLYVVKGRSDEKAIPILLSDIAQLALVALEVSESVRRLADRFWPGPLTLVLPK
                     QPTLPAEISSQRTIGVRMPDHPIALALMRQTGPLAVTSANLSGGENSVTALQVMAQLG
                     GLIPLILDGGKTPGGSPSTVVDCTAVEPRILRSGPISLDQILEALS"
     gene            complement(990..3242)
                     /locus_tag="AC16CB_06975"
     CDS             complement(990..3242)
                     /locus_tag="AC16CB_06975"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3342247.1"
                     /translation="MQSFGFIELLAIAQTVVYLLVAVLALWRQKAQPHQALYALVSVL
                     WLAAQLALIQGWLPFLLDDFTARLPLYGLLLLSVTLLWFARAYLRVEEGSWGWRLIGL
                     AWIATLLLVDSNLVAVPEEMWFRGGLMLPRDNFIVGGIVLGWCFFTGAATLMTLLVYR
                     RTRQPLQRNRIRYLAAVLFLVFTGEAVFLFAYWSLGSLLHLSGVLVGAYATLNARLPS
                     FRRAVLRSLSYLITSGLALLFYLAALMLSYATLRATGEANLWLVGLVLAAALAVFFTP
                     LLGQAQKWLDRLVAGVSHDPALILRQYSQTISNILDLGLLAKVVTELIGQALDIPRSY
                     LFTVSHEKDSEGHNSYRLSEVMDEDGDEPKDIILAEASTLCEYLHHQRGPLTQSEIDS
                     SPRFAEITPDERGWLLDLQMELFVPIHSKDEWIGLLALGPKNSGEPFWDDDQSLLASL
                     ADQTAVALENARLVESLVRLNNDFRRAIAALDQANRNLERLDRTKTEFISVASHELRT
                     PLTLINGYSQVLMEEPSLAGNANYAKMISGIHSGAQRLQEVLESMLDMAKIDTRELQL
                     NLQTVHMGELIESLRAEFDTALQERNQKFETVDLASLPSIQADREALRKVFYHLLVNA
                     IKYTPDGGKIAVSGRAVLPSKDTLIESGVEIIVSDTGIGIDPSLHDVIFTKFYQTSDL
                     ALHSSGKTKFKGAGSGLGLAIAKGIVDAHFGRIWVESEGYDETRCPGSQFHVFLPLRH
                     PEKTRPKAAI"
     gene            complement(3255..4568)
                     /locus_tag="AC16CB_06980"
     CDS             complement(3255..4568)
                     /locus_tag="AC16CB_06980"
                     /inference="COORDINATES: protein motif:HMM:NF020084.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FIST signal transduction protein"
                     /protein_id="HIR3342248.1"
                     /translation="MTVRSGFEVPGSMNLGSDSRDIFMEDRMFFISATTTETGVEEAV
                     SSLFEQVISQARPHLGDDPQSDWPHLVIVFVSAHFIHDARRIVEEFHARLHPRVLLGC
                     TADGVIGWDQEIEEVPAITLIAAHLPGVEVSPFLLQPLDADPPALASWHKLLWDSDEF
                     QRTVGAPQDTRLFILLGEPFSTPMDDLLQAFNANFKGIPVVGGMASGALRPHGNALWV
                     KDHLTTEGVVGVALAGEIDVDIIVSQGCRPIWRPFQVVSAHRNAILSLEGRPPLAWIQ
                     ELIPELSEEDRLLLQNGLFVGRFIKPTSETIGRGDFLVRGVIGVDQESGAIAIGDSII
                     EGEMIQFHLRDALTAMEDLEMMLIPQGFRHPASGALLFSCNGRGTRLYDHPNGDISVI
                     QATLEGTPLAGFFCAGEIGPLGDDNFLHGHTASLVIFRPIVKQEG"
     gene            complement(4603..5340)
                     /gene="ppgK"
                     /locus_tag="AC16CB_06985"
     CDS             complement(4603..5340)
                     /gene="ppgK"
                     /locus_tag="AC16CB_06985"
                     /EC_number="2.7.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0047330 - polyphosphate-glucose
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate--glucose phosphotransferase"
                     /protein_id="HIR3342249.1"
                     /translation="MKEILGIDIGGTGIKGAPVDVENGSLLAPRFRLLTPSPSKPKPM
                     AETVAEIARHFAWTGPIGCGFPALVQAGVVRSAANIHKKWIGVDAQALFADVTGCPVI
                     VLNDADAAGLAEMTFGAGRGRNGVVMIITIGTGLGTALFLDGQLVPNTELGHIEIGGQ
                     DAELRASDAARQRDKLSWKRWAKRFNKYLNTLERLFSPDLFILGGGASKKHEKFIPLL
                     SVRAEILPAQLLNEAGIIGAALAARTV"
     gene            complement(5423..6823)
                     /locus_tag="AC16CB_06990"
     CDS             complement(5423..6823)
                     /locus_tag="AC16CB_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HIR3342250.1"
                     /translation="METSHLQPSELLAQADTLRRGLSAGFRDETVRSIYIEAERIARR
                     AVHTKADQRWDFDQRIDRLITSPYLGLPIMGLILAVVFWLTIVAANVPSQLLANALFW
                     IEERASALFDAWGVPWWITGFIWHGIYRGLAWVVSVMLPPMAIFFPLFTIFEDLGYLP
                     RVAFNLDFLFKKAGAHGKQALTMTMGFGCNAAGVIATRVIDSPRERLIAILTNNFVPC
                     NGRFPTLIMLANVFVAAAFPPVFASLAAAGSVVGVVLIGVFFTLLVSWLLSRTLLKGE
                     ASAFTLELPPYRRPNIGRIMYTSLIDRTIFVLWRAIQTAAPAGAVIWILGNVYAGGTS
                     LAQHVASFLNPLGLLIGLDGVILLAYVIAIPANEIVVPTMMMVYMGTGMMTDIPSLEG
                     LRSLLVGGHGWTLLTAVNLMLFALLHNPCATTILTIYKETRSRKWATIGALMPLGLAF
                     LVTFITASLARIFGWV"
     gene            complement(6915..7727)
                     /locus_tag="AC16CB_06995"
     CDS             complement(6915..7727)
                     /locus_tag="AC16CB_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HIR3342251.1"
                     /translation="MSEPCENCPANVHLAQMGLKLDKFDRVVALAGNPNTGKSTLFNA
                     LTGLRQHTGNWPGKTVTRAEGGFEFNGVRYKLVDLPGTYSLLSASQDEEVARDFILFG
                     QPDCTIVVTDATALERNLNLVLQVLEITDRVVVAVNLMDEAQRKGLEVDVRSLARDLG
                     VPAVPVTARTGEGIHGLLSAVEGLISGEIPTKPRRVEGTPEFQRAVNELVPMIESLAP
                     GVPNARWLAIRLLDGDTRVRQAILNGELAELVAHQRQADVSFSRKIALEGTQ"
     gene            complement(7794..>8750)
                     /locus_tag="AC16CB_07000"
     CDS             complement(7794..>8750)
                     /locus_tag="AC16CB_07000"
                     /inference="COORDINATES: protein motif:HMM:NF015956.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIR3342252.1"
                     /translation="LLARWRVNSPAGQKELVEDALKFLLKQEREGRYASPEALAGTLR
                     LYGNDLLRLMTRMETQGLLLSADGGLRLTPDGERLALHVVRAHRLLERYLADEARLPL
                     EKVHSEAERREHGLTPEQLDDLAASLGYPDLDPHGDPIPDQQGEMVPAAGTSLTSWTA
                     GVQGRIAHLEDEPPLAYDQILAEGLRLGQVVHILETTPARIVLSDGENEYRLAPAVAA
                     NIYVSPLPESLESQSGVMPLSELPDKMEAEVVALDEACQGFTRRRLLDLGMTPGVRLV
                     AEMRNFFGDPRAYRVRGTLIALRREQAASIWVRPVERQAAEG"
BASE COUNT         1968 a         2639 c         2406 g         1737 t
ORIGIN      
        1 tggtggcgcg cttccgcaag gagctggggc gagaaatgaa agtcatcgcc accggcgggc
       61 tggccgaggt catcgctcgc gaaaccgctg tgctcgagat catcgctccc tggctcacct
      121 tggacggtct gcggattatc tgggagctga accaatcgaa atgacaaaga ccgttatcct
      181 ccccgccgat gacccggttt ctaccaggcg ggcgcttgaa atattggggc aagggggtct
      241 ggtagcattc cccactgaca cggtctacgg cgtaggcgca ttggtgagca aggcgcaggc
      301 catcgcgcaa ttgtacgtgg tgaaagggcg cagcgatgag aaagccatcc ccatcctgct
      361 cagcgacatc gcacaactgg cgctggtagc gctcgaagtg agcgagagcg ttcgccgact
      421 ggcagaccgc ttctggcccg gcccgctcac actggtgctg ccaaaacagc ccacgctgcc
      481 ggctgagatc tcttctcagc ggaccattgg cgtgcgcatg ccagaccatc ccatcgcgct
      541 ggcgctcatg cgccagactg gtccgctggc ggttacgtct gccaacctat ccggtggaga
      601 aaactccgtg acggccttgc aggtgatggc gcagctcggc gggctcatcc cgctcatcct
      661 ggatggtggc aagacgcccg gcggcagccc ttcaacagtg gtggactgta ccgcggtgga
      721 gccgcgcatc ctgcgttctg gcccgatcag tttagatcaa atacttgaag ctttgtcttg
      781 atctccagct ctgagaaact ggacaaatcc ccctttttct atcttctaac caaactttta
      841 tcttctcttc atgactcttt tagctatgtg tatataatgg tagttgcatt ctcctcactc
      901 aaaactgcgc ttcggagcta ggtgccaggc cttctcccag gcgcagtttg tttttaacaa
      961 agatctcgaa ttgaatcccg aatgagttat taaattgccg ctttcgggcg agtcttctcc
     1021 ggatggcgca aaggcaaaaa aacgtggaac tggcttccag gacagcgagt ttcatcatac
     1081 ccttcgcttt ccacccagat tctcccaaaa tgggcatcca caataccttt ggcgattgcc
     1141 aatccaagcc ccgacccggc gcctttgaac ttagttttcc cgctggagtg tagagccaga
     1201 tcgcttgtct ggtaaaactt ggtaaagatt acatcgtgca ggctggggtc gatcccaatg
     1261 ccagtgtcac tgacaataat ctccacgccg ctctcgatta aggtgtcctt tgacggtaaa
     1321 acggcgcgcc cgctgactgc gatcttaccg ccatcggggg tgtacttaat agcattgacc
     1381 agcaaatgat aaaaaacctt acgcaacgcc tcgcgatcgg cttggataga cggcagtgaa
     1441 gccaggtcaa ccgtctcgaa cttctggttg cgttcttgta atgccgtatc gaactccgca
     1501 cgcaacgatt cgattaattc ccccatatga acagtttgca ggttgagctg cagctcgcgg
     1561 gtgtcgattt tagccatatc cagcatgctc tctaaaactt cctggagacg ctgcgctccg
     1621 ctgtggatgc cagagatcat cttggcgtag ttggcatttc cagccagaga tggctcttcc
     1681 atcaacacct ggctataacc attaattaat gtgagaggcg tgcgcagctc gtgagaggcc
     1741 acgctgataa attctgtctt ggtgcggtcc aatcgctcca ggttgcggtt agcctgatcc
     1801 agggcagcaa tcgcccggcg aaaatcgttg ttcaagcgca ccaggctttc caccaagcga
     1861 gcgttctcca gcgccaccgc cgtctggtca gccaagctgg caagcaagga ttgatcatca
     1921 tcccaaaagg gctcacccga atttttgggc cctaaggcca gcaaaccgat ccattcatct
     1981 ttggaatgaa tcgggacaaa gagctccatc tggagatcca aaagccaacc gcgctcatcg
     2041 ggcgtgatct cggcgaagcg tggcgaggag tcgatctcgc tttgggtgag tgggccgcgc
     2101 tggtgatgca aatattcgca cagcgtgctc gcttcagcga ggatgatatc tttcggctca
     2161 tccccatctt cgtccataac ctcgctcaga cgataactgt tatgcccctc ggaatccttc
     2221 tcatggctga ccgtaaacag gtagctgcgc gggatatcca acgcctgacc tatcaattcc
     2281 gtcacaacct tcgcgagcag ccccaggtca aggatattgc tgatggtctg gctgtactgg
     2341 cgcaggatga gcgccgggtc gtgactgacc ccagcgacca ggcggtcaag ccatttctgc
     2401 gcctgtccaa ggagaggggt gaagaagact gccagggcgg cagccagcac caggccgacc
     2461 agccaaagat tggcctcccc tgtagcccgg agagtcgcat acgagagcat cagagccgct
     2521 aggtaaaaga gcaaggccag cccgctagtg atcaggtagc tcaagctgcg cagcacagcc
     2581 cgtcgaaatg aaggcaagcg cgcgttaagt gttgcatagg ctcccaccag tacacctgag
     2641 agatgcagca ggcttccaag agaccagtaa gcgaacagga aaaccgcctc gcccgtaaag
     2701 accaggaata aaactgctgc cagatagcgt atacggttgc gttgcagtgg ttgtcgggtg
     2761 cgccggtaaa ctaacaaggt catcaaggtt gctgcaccag tgaagaagca ccatcccagc
     2821 acgatgcccc ccacaatgaa gttgtcgcgc ggcagcataa gcccgccccg aaaccacatt
     2881 tcctcgggta ctgccaccag gttactatcc accaggagca atgtggcgat ccaggctagc
     2941 ccgattaatc gccaccccca gctaccttcc tcgacacgca ggtaggctcg tgcgaaccaa
     3001 agaagggtta ccgacagtaa cagcagtcca tacagcggca accgggctgt aaaatcatcc
     3061 agcaagaaag gcaaccagcc ttgaatcaaa gccagttgcg ccgcaagcca taggacagag
     3121 accaaggcat aaagcgcctg gtggggttgg gctttctgcc tccaaagagc caggacggca
     3181 accaacaaat atacgactgt ctgagcaata gctaaaagct ctataaagcc aaacgattgc
     3241 atgttgttta gaaattatcc ctcttgtttc acaatagggc ggaaaatcac caggctggca
     3301 gtgtgcccgt gcagaaagtt atcgtcaccg agcgggccga tctctccagc gcagaagaat
     3361 ccagccaggg gcgtaccttc cagagtcgct tggatcacgg agatatcccc gttcggatgg
     3421 tcgtacaggc gcgtgccgcg cccattgcat gaaaacagca acgctccact ggcaggatgg
     3481 cggaaacctt gggggatgag catcatttcc aggtcttcca tggcggtcag ggcatcgcgc
     3541 aagtgaaact gtatcatctc cccttctata atggaatcgc caatcgcgat ggcaccgctc
     3601 tcctggtcca caccgatcac accgcgaacg agaaagtcgc cccgacctat tgtctccgat
     3661 gtaggcttga tgaagcgccc tacaaacagg ccattctgca acaggaggcg atcttcttcg
     3721 gaaagctcag ggatcagttc ctggatccac gccagagggg ggcggccttc caggctcaag
     3781 atcgcgttcc ggtgggctga gaccacctga aacggtcgcc aaatggggcg gcagccctgc
     3841 gaaacaatga tatccacatc tatctcccca gcaagcgcca ctccgacaac tccctcggtc
     3901 gtgaggtgat ccttcaccca caaggcattt ccatgcgggc gcagtgcgcc actcgccatg
     3961 cccccaacca ccgggatacc cttaaagttc gcattaaagg cctgcaataa gtcgtccatt
     4021 ggtgtagaaa aaggctctcc cagcaggatg aacagccgcg tatcctgcgg cgcacccacg
     4081 gtgcgctgga actcatccga atcccagagc aatttgtgcc agctagccag cgcaggcggg
     4141 tcggcatcca agggctgcaa gaggaagggc gaaacctcca cgccgggcaa atgcgccgcg
     4201 atgagggtga tcgccggtac ctcctcaatc tcctggtccc atccgatcac gccgtctgct
     4261 gtgcagccca acagcacccg ggggtgcagc ctggcatgga attcttcgac aatccgccgg
     4321 gcatcatgga taaaatgagc agacacaaac acaattacca ggtgaggcca gtctgattgt
     4381 ggatcgtctc ctaggtgagg gcgcgcctgg cttatgacct gctcaaacag ggagctgaca
     4441 gcctcttcaa ccccggtctc ggtggttgta gcggagataa agaacatcct gtcttccata
     4501 aaaatatctc tgctgtcgct acccagattc atggagcctg gcacttcaaa ccccgacctt
     4561 acggtcatac caagctttac aggaatccgc tttctgaagt ttttatacag tgcgcgcggc
     4621 taacgctgcc ccaatgatac cagcctcgtt gagcaattgg gcgggcagaa tctcggcccg
     4681 cacactgagc aaggggataa acttttcgtg ctttttgcta gcccctccac ccagaatgaa
     4741 caggtctggc gaaaacaggc gctcgagcgt attcaggtac ttgttaaagc gcttggccca
     4801 gcgtttccag gagagcttat ctcgctggcg agcggcatcc gaagcgcgca actcggcgtc
     4861 ttggcctccg atctcaatgt gccccagctc ggtattaggg actaactgtc catcgaggaa
     4921 gagtgcggtc cctaacccgg tgccaatggt aatgatcatc accacaccat tgcgccctcg
     4981 cccggcaccg aaggtcatct ccgccaaccc ggccgcgtca gcgtcattga ggacgatcac
     5041 cggacagcca gtcacgtcag caaacagggc ctgcgcatcc acgccgatcc attttttgtg
     5101 gatgttggct gcgctgcgca ctaccccagc ctgcaccaac gcaggaaatc cacagccaat
     5161 cgggccggtc caggcgaagt ggcgagcgat ctcagccacc gtctctgcca tcggctttgg
     5221 cttggatgga gagggggtca gcaagcgaaa gcgcggcgcc agcaagctgc cattttccac
     5281 atcgaccgga gcgcccttga tgccggtacc gccgatatcg attccgagga tctctttcaa
     5341 aatgcctcct ttacgggcaa accgcgtctg ccccgatctc tactaactcg ccttaatcaa
     5401 tacgcgattt tacctctact ttttataccc acccaaaaat tcgcgccagg ctagcggtga
     5461 taaacgtgac cagaaatgct agccccagcg gcatcagtgc gccgatagtc gcccattttc
     5521 ggctgcgggt ttctttgtaa atggtcagaa tggtggtggc acagggatta tgcaacaatg
     5581 caaataacat caagttcacg gcggtcagaa gggtccagcc atggcctccc accagcaggg
     5641 agcgcaatcc ctccagcgaa gggatatccg tcatcatgcc agtgcccata tacaccatca
     5701 tcatggtcgg caccacaatc tcgttagcag ggatggcgat gacataagcc agcaggatca
     5761 cgccatccag cccgataagc agacctaacg gattcaggaa gctggcgacg tgctgcgcca
     5821 ggctcgtgcc gcctgcatat acattgccca agatccagat caccgcgcca gccggggcag
     5881 cggtctgtat cgcccgccag agcacgaaga tggtgcggtc aataagcgaa gtgtacatga
     5941 tgcgcccgat attaggtcgt cgatagggcg gcaattccag ggtaaaggcg gaagcctcgc
     6001 ctttcagcag cgtgcgcgag agcagccaag agaccagcag ggtgaagaac acgccgatca
     6061 gcaccacgcc taccaccgag ccagccgccg ccagggaggc gaaaaccggc ggaaaagccg
     6121 ccgccacaaa gacgttcgcc agcatgatca gcgtgggaaa gcgcccattg catggcacga
     6181 agttattggt caggatggcg atcaggcgct cgcggggcga atcgatcacc cgcgtagcga
     6241 ttaccccagc tgcattgcag ccaaagccca tcgtcatcgt cagggcctgt ttgccgtggg
     6301 cgccggcttt cttgaagaga aaatcgagat taaatgcaac gcgcggcagg tagcccaggt
     6361 cctcgaagat tgtgaacagg ggaaagaaga tcgccatggg cggcagcatg acgctcacca
     6421 cccaggctag cccgcggtat atgccgtgcc agatgaatcc ggtgatccac cagggcacgc
     6481 cccaggcatc gaacagggcg ctggcgcgct cctcgatcca gaataaggca ttcgccagga
     6541 gctgggaggg gacattggcg gcgacaatgg tcagccagaa tacgaccgcc aggatcagcc
     6601 ccatgatggg caggccaagg taaggcgagg taatcagccg gtcgatgcgc tggtcaaagt
     6661 cccagcgctg gtccgctttg gtgtgcacgg cgcgccgggc gatgcgctcg gcctcgatgt
     6721 aaatcgaccg cactgtctca tcacggaatc cagcgctcag gccgcggcgc aaggtatctg
     6781 cctgcgcgag cagttcggag ggttgaaggt gggatgtttc catggtctcc attccaagta
     6841 ccaagttcca tgttccaagt tccaagcgct cagtccacac aaaacgtggt acctgacact
     6901 tggtacttgg tactttattg cgtcccttcc agggcgatct tacggctaaa gctcacgtct
     6961 gcctggcgtt ggtgggcgac cagttcagcc agctcaccgt tgagtatggc ctggcgcacg
     7021 cgcgtgtcgc catccagcaa gcggatagcc agccagcgcg cattgggcac acctggcgct
     7081 agactctcta tcatcggcac cagctcgttg actgcacgct ggaactcggg cgtgccctcc
     7141 acgcggcgcg gcttggtggg gatttcacca ctgatcaatc cctccaccgc gctaagcagt
     7201 ccgtggatgc cctccccggt acgggctgtc accggtacgg cgggcactcc caggtcgcgc
     7261 gccaggctgc gcacatcaac ttccagaccc ttgcgctgag cctcgtccat taaattgaca
     7321 gccaccacaa cccggtcggt gatctccagc acttgcagca ccaggttcag attgcgctcc
     7381 agggcagtgg cgtcagtaac tacaatagtg cagtcaggct gcccaaagag gataaaatca
     7441 cgcgccactt cctcatcttg ggaggcggat agcagcgaat acgtgccagg caaatccacc
     7501 agcttatagc gcacgccatt aaactcaaac ccgccttcgg cgcgggtcac cgtcttgccc
     7561 ggccagttgc cggtatgctg gcgcaagccg gtcaaggcgt taaacaaggt cgattttccg
     7621 gtattggggt tgcccgccag ggctaccacc cggtcgaatt tatccagctt taggcccatt
     7681 tgagccaaat gcacgttggc agggcagttc tcacaaggtt cgctcataga tacatcctaa
     7741 ttacataatg tcgtatgtta atcttttaga ttgcagtttg actccctgct caattatcct
     7801 tcagcagcct gccgctcaac aggcctgacc catatgctgg ctgcttgctc gcggcgcaga
     7861 gcgatcagtg taccgcgcac ccggtaggca cgcgggtcgc cgaagaaatt gcgcatctcg
     7921 gcaaccaggc gcactccagg tgtcatcccc agatctagca agcgccggcg ggtaaaaccc
     7981 tggcaggctt cgtccagcgc caccacttca gcttccatct tatcgggcag ctccgagagg
     8041 ggcatcaccc cagattgcga ttcaagggac tctggaagcg ggctcacgta aatgttggcg
     8101 gccaccgctg gggcaaggcg gtattcgttc tcaccgtcgc tcagcactat tcgtgcaggg
     8161 gtggtctcta agatatgcac cacctgcccc agacgcaacc cctccgccag aatctggtcg
     8221 taagccaggg gaggctcatc ctccagatga gcaatgcgcc cctgaacgcc agcagtccag
     8281 gaggtcaggg acgtgccggc tgcggggacc atctcgccct gttggtcagg gattggatcg
     8341 ccgtgcgggt ccaggtctgg atagcccaag gaggctgcga gatcgtcaag ctgctcgggg
     8401 gtcagaccgt gctcgcggcg ctcggcttct gagtgaactt tctccagagg caggcgggcc
     8461 tcgtcagcca gatagcgctc taaaaggcgg tgggcacgca ccacatgcag cgccaagcgc
     8521 tcaccgtctg gggtcaagcg cagcccgcca tctgccgata gcagcaatcc ctgggtttcc
     8581 atgcgggtca tcaagcgcag caggtcgttt ccatagagcc gcaacgtgcc cgccaacgct
     8641 tctggcgagg cgtagcgccc ttcacgctct tgtttgagta ggaatttcag ggcgtcttcc
     8701 accagctcct tctgccccgc cggactgttc acccgccagc gcgccagcag
//
LOCUS       DBNGSX010000232        14018 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1334297, whole genome shotgun sequence.
ACCESSION   DBNGSX010000232 DBNGSX010000000
VERSION     DBNGSX010000232.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14018)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14018)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14018
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1334297"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="AC16CB_07005"
     CDS             complement(<1..713)
                     /locus_tag="AC16CB_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HIR3342253.1"
                     /translation="MNDRVAIIGVGWAGFRPITPDLSYKELIYEAAVKAYSDAGVDPR
                     RDVQSFVTVAEDFHEGTSIFDEYTPDQIGAALKPVHTITGDGLHGLATGAMLIRTGMF
                     DLVAIEGHSKASNILTLPEVTAYAQDPVLNRPLRLNTHFVAGMEMNRYLYETGTTPEQ
                     CAAVVVKNRRNALRNPSAPYGADLTLEEVLSGQPLSWPLNRRETAEHSDGAIVMVLAS
                     EEKARALSDNPIWILGLGW"
     gene            complement(710..1600)
                     /locus_tag="AC16CB_07010"
     CDS             complement(710..1600)
                     /locus_tag="AC16CB_07010"
                     /inference="COORDINATES: protein motif:HMM:NF026954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HIR3342254.1"
                     /translation="MRARRALLYMPGDDFHKIRKATTLGVDCICMDIEDGVAFNRKGE
                     ARKTILEALHTLDFGQAERLVRINPVGSGLELEDLLAGLEGHPDGIVVPKVENSDQVR
                     WASQSIASIEKKFGWPAGEIILIAIVETASGIINLPHIAAADPRLQALIFGAEDLAGD
                     IGAIRSHEGWEVFYARSAVVTHAAAFNLQAIDMVYLDFQDVDGLVQECQLGARMGFSG
                     KQVIHPNQVEPVQSAFTPSDEAISHALRLMEAYAAHQQSGRGAFALEGKMVDAPIIKT
                     AERVLERARAAGKILKGGQA"
     gene            complement(1603..2475)
                     /gene="tatC"
                     /locus_tag="AC16CB_07015"
     CDS             complement(1603..2475)
                     /gene="tatC"
                     /locus_tag="AC16CB_07015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HIR3342255.1"
                     /translation="MRTLLRAIWLIISAPFRFVIWIFRTIARWIAVITSEIRQLFTAE
                     VEDEPLPDTLAKTIENPAGLLEHVDALRKHLTRAVIFLALTTALAFAFTRQIIDYLAL
                     PIGGIGKLTPIEVTEPVGVFMRVALLVGFSLALPYIAFELWLFAAPGLHRRSRFTGLI
                     AIPLVAIFFLAGMAFAYFAMLPTALPFLLNFLGMNPQVRPASYISFVTSLMFWIGVSF
                     EFPLVIYVLASIGIVNHKMLIDQWRLAVVVIAILAASITPTVDPVNMTLVMAPMTVLY
                     FLSIFLANLARRGR"
     gene            complement(2478..2894)
                     /locus_tag="AC16CB_07020"
     CDS             complement(2478..2894)
                     /locus_tag="AC16CB_07020"
                     /inference="COORDINATES: protein motif:HMM:NF014470.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033281 - TAT protein transport complex
                     [Evidence IEA];
                     GO_function: GO:0008320 - transmembrane protein
                     transporter activity [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA/TatB"
                     /protein_id="HIR3342256.1"
                     /translation="MDFLGIGPLELLFVVIIALIVLGPKDMVKAGKTIGRLLRSIVTS
                     PNWRAIQQTSQEIRHLPNRLMREAGMEEIQKNLPRTDEIARKIGINEVSKDISQVGKG
                     LSDWTTPPETNSSSKPDEENKILPPSIQSEEINSED"
     gene            complement(2901..3377)
                     /gene="nusB"
                     /locus_tag="AC16CB_07025"
     CDS             complement(2901..3377)
                     /gene="nusB"
                     /locus_tag="AC16CB_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR3342257.1"
                     /translation="MKPRTRARSVALQALYEIDVTGHAPGSVWEQRVEEEGLADSLAD
                     FAREIIFGVFPLMATLDEFIAEHAPEWPMDQVATIDRNILRIALWEIAVLGKTPVKVA
                     INEAVELAKVYGSDSTPRFVNGVLGSLASRQHEIQQSFNRLAQNHPNNNNHLGDSG"
     gene            complement(3384..3725)
                     /locus_tag="AC16CB_07030"
     CDS             complement(3384..3725)
                     /locus_tag="AC16CB_07030"
                     /inference="COORDINATES: protein motif:HMM:NF015721.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIR3342258.1"
                     /translation="MDEGKTTIAPEVLITIAQLTTLNVPGVSRMCIDPPGLNRIFKRN
                     FHEGIRIEVRGDTVFADIHVVLNRDVNLREVSRNVQQQVTRAISEMVGMQVGSVNVHI
                     EDIDYARENEA"
     gene            complement(3741..4505)
                     /gene="fabG"
                     /locus_tag="AC16CB_07035"
     CDS             complement(3741..4505)
                     /gene="fabG"
                     /locus_tag="AC16CB_07035"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HIR3342259.1"
                     /translation="MSEPARLSLEGRVAVVTGASRGIGRAIALELARLNAAVIVNYNT
                     SEKAAEEIVNQILTGGGQAKSVRADVSDFQQAQELIKTAIDTFGGLDILVNNAGITRD
                     MLIMMMTEEDWDRVQNTNLKSTFNCSKAAVRHMMRKRYGRIINITSVAGQMGNAGQTN
                     YSASKAGQIGFTKALAREVASRNITVNAIAAGYIDTEIWANVPEEARQNALTIIPLGR
                     KGQPEEIAYAAAFLASEQAAYITGQILGVDGGMAMM"
     gene            complement(4666..5613)
                     /gene="fabD"
                     /locus_tag="AC16CB_07040"
     CDS             complement(4666..5613)
                     /gene="fabD"
                     /locus_tag="AC16CB_07040"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR3342260.1"
                     /translation="MKPNKTAFIFPGQGSQFMGMGQLLCDKYTVAREAYELADEWLGF
                     PLSKFAWEGPEETLNDTINTQPALMVHSVAALRVFRELISEFRPAYVAGHSMGELSAL
                     VASEALPYREALHLVRRRGELMKRAGIVSPGGMAAVLGLDIPTLDEICAQASTQDEVV
                     QVANDNCPGQVVISGASSALERAIGLAQEAKARRVVPLPISIAAHSPLMINAQEDFNI
                     AVDSSPIQDPVIPIIGNVDARPLASAEHIKVDLQAQLTSRVRWTESVQYMISHGIDTF
                     IEIGSGSVLGGLIKRIDRQVQTLTLGTPEDFEKLISALP"
     gene            complement(5777..5968)
                     /gene="rpmF"
                     /locus_tag="AC16CB_07045"
     CDS             complement(5777..5968)
                     /gene="rpmF"
                     /locus_tag="AC16CB_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIR3342261.1"
                     /translation="MPPQPKRKLSKGRRDRRRAHDALEAHNLVPCSNCGEMRLPHTVC
                     PSCGHYKGREIIEVEEKKS"
     gene            6191..7171
                     /locus_tag="AC16CB_07050"
     CDS             6191..7171
                     /locus_tag="AC16CB_07050"
                     /inference="COORDINATES: protein motif:HMM:NF018190.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HIR3342262.1"
                     /translation="MKHFKVHATIVIVLLFGAAACNIPVGPQPIIPQQTPDLTITALF
                     EFITTAQAEQTNVAVPTSAPPTETASVTPPPVQATATTSAPPTPTNTALPTDTKVPTK
                     VVSSGGRERSGSSVIAYYIQKEPTIDGVFDEWDFDRYSVSRVVYGDNRWNGNDDLSAT
                     LMIGWDDYYLYLAVRVKDDIYVQKASGKNLFKGDSIEILLDANVSRDYNDNDLSNDDY
                     QIGISPGQSTPSDDIETYLWYPSSKAGEYTSIKAAALPTDDGYRIEAKIPWEIFGITP
                     DIGKHFGFAFSVSDNDKSSENVQQSMVSNVSTRHLTDPTTWGDLTLEGRR"
     gene            complement(7279..8112)
                     /locus_tag="AC16CB_07055"
     CDS             complement(7279..8112)
                     /locus_tag="AC16CB_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012314.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3342263.1"
                     /translation="MMASDNIIDVSEADFEYQVLAYSQHIPVVVDFWAEWCVPCRTLG
                     PMLERLTLEAGGAFRLAKVNVDQNQNLSLRYNVRSIPAVKAFRDGKMVSEFVGMQPEP
                     KLKEWLRALAPSSTDLLLEKGLSLISEGEWVSSQEAFQEILKVNPDSAPALFGLAKSI
                     LAQGQVDEAQRILQSLPPSREFARAEALLPLTQALIRQQNGENNHDQPLEAAYQRALR
                     LILRGNIPASLDGLLDILREDKYFRDGEVRQVILGLFELMGDENPVTRQYRQELASVL
                     F"
     gene            complement(8226..8651)
                     /locus_tag="AC16CB_07060"
     CDS             complement(8226..8651)
                     /locus_tag="AC16CB_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342264.1"
                     /translation="MSRQAIFSGLVIDENDNLVQVRDVGDEPCYVVDDAGFLRHIPSE
                     LVDRQVLEAMGESMQGHEDLISEQAAKMLGQEDIFSIAMIANQIKNIDKQLDSLIETG
                     IPEDVRAYLGMMGFKVRINVHGDVLEVVQPGSAGPPDIE"
     gene            complement(8656..9240)
                     /locus_tag="AC16CB_07065"
     CDS             complement(8656..9240)
                     /locus_tag="AC16CB_07065"
                     /inference="COORDINATES: protein motif:HMM:NF016413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HIR3342265.1"
                     /translation="MMHSAFLYNQYFLKRQVLALTGKIRLYSPDGSLLLYSQQKMFRL
                     REDIRVYNDESRSQELLYIQARNIIDFSAAYDVIDSLSGIKVGVLRRKGLRSLLRDQW
                     EILDASDQPQAILQEDNPTRALLRRLLLGTLLPQNYDVISGETRLADFRQRFNLFRYE
                     LELDFSMDSARRLDRRVGLAAAILLGIIEGRQES"
     gene            complement(9241..10290)
                     /locus_tag="AC16CB_07070"
     CDS             complement(9241..10290)
                     /locus_tag="AC16CB_07070"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIR3342266.1"
                     /translation="MNTTRQNQDAKIARSISRREFIKLGGAGVLGFLLPGEHADQKTP
                     LEQQGRVLEDGIQLYDIPSFHGRELQKFWKDMVVPISEATIGDSEPAHNRVWYAINGE
                     GFVHSGSIQPVSTELNPELADIPNGGTVAEVTVPYTDAHWRPDKKYTVAYRLYYETTH
                     WVTGLVYDNLGQAWYRIAEDKWKLKYFAPATHLRILPAEELNPISPDLPPFAKRIEVR
                     TQEQVVIAYEWDQPVFMAKTATGAQFSNGNFATPPGRHITAHKRPSRHMAAGNLAYNG
                     YDLPGVPWICYITESGISFHGTYWHNNYGRPRSHGCINLSPKAAKWIYLWTMPMVPPR
                     DQFIRKDFGTIVDVI"
     gene            complement(10295..11254)
                     /locus_tag="AC16CB_07075"
     CDS             complement(10295..11254)
                     /locus_tag="AC16CB_07075"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIR3342267.1"
                     /translation="MAQKCVKLQPQREEGWLILGALASPKGSVNYLQHALSINPASQR
                     ARKGMHWAIQRLRKNNTAADAPAPIPRTRVAATITSDSMARQRPLLMPWAIAITLLVL
                     GLAIWFGSPTFSLAFNNGKQLSLAQVQLSKQTRTPTPTATFTPTPTSTSTPTPTITPS
                     PTPTETPSPTPTDTPKPTKKPKKNNVAKENYNYPGRPRGVDGNERWIDIDLSSQRVYV
                     YQGDQLVNKFVVSTGTWQTPTVKGTYKIYVKYRAADMSGPGYYLPKVPYVMYFYKDYG
                     LHGTYWHNNFGRPMSHGCVNLKTKDAGWLYDFASVGTVVNVHQ"
     gene            complement(11338..12129)
                     /locus_tag="AC16CB_07080"
     CDS             complement(11338..12129)
                     /locus_tag="AC16CB_07080"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR3342268.1"
                     /translation="MSTESSPPQPVSPAKPSRGSFYYIQVVVGIAFVLATLFTLWTDP
                     GMLPSDLAENFTFAQAPGVTGTPETPITPTPRGKPLIGIVAGHKGNDSGSVCADGLTE
                     ASVNEKVAYLVQKDLVDNGYDVEVLNEFDPQLSDYQASVLVSIHSDSCDYVNNQATGF
                     KVSSALANPHPDRAARLTACLKNRYGIVTGLPLHNSITIDMTSYHAFAEISDNTTAAI
                     IEIGFLNLDRELLTQKSDLVAKGIFDGILCYINNEDLIVNATPTP"
     gene            complement(12277..12621)
                     /locus_tag="AC16CB_07085"
     CDS             complement(12277..12621)
                     /locus_tag="AC16CB_07085"
                     /inference="COORDINATES: protein motif:HMM:NF013999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HIR3342269.1"
                     /translation="MSEETSSTLIWQVDSTHPELSAPLRDALREVVDPEIGLNIIELG
                     LVRDVVVEEDSAKITMILTTPFCPYGPALLDITRQKGEKALQRSTTIEMGIEMWDFSM
                     MEEGAGAAWGFY"
     gene            complement(12618..13280)
                     /locus_tag="AC16CB_07090"
     CDS             complement(12618..13280)
                     /locus_tag="AC16CB_07090"
                     /inference="COORDINATES: protein motif:HMM:NF013209.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3342270.1"
                     /translation="MATIVRDEEKSKSTRQRVMQTLLSRQRCTINDLAEAVEINPISV
                     RHHITRLEAEGLVNSEEERHGVGRPRRLYFLTEKGLERFPSRYLRFTIRLLEQLKDTM
                     PEAMVSSLFRQMAKDVAADYESMVNGLNMEERIDLVAQLLSSEGFTVEWERKGSEYHI
                     REISCPYYHIGQSHPEVCSVDQTLISTMLSVPAEKVKCVLNGDTNCTYVVSSMASLEN
                     NQ"
     gene            13516..>14018
                     /gene="sufC"
                     /locus_tag="AC16CB_07095"
     CDS             13516..>14018
                     /gene="sufC"
                     /locus_tag="AC16CB_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HIR3342271.1"
                     /translation="MSELSIRNVHVSIDGKEILKGVDLTIRQGEIHALMGPNGTGKST
                     LAYALMGHPNYKITDGEVWFKGQNILDLEPDQRSHLGIFLAFQYPVAIPGVSVANFLR
                     SALNARRKADNPEDKGVPIPEFRKLLKERMDLLKMDHSFAGRYLNEGFSGGEKKRAEI
                     LQMAALRP"
BASE COUNT         3500 a         3365 c         3472 g         3681 t
ORIGIN      
        1 atccagccaa gacctaatat ccagattgga ttatcagaca aggcgcgcgc tttctcttcg
       61 gaggccaata ccatgacgat agcgccatcg gaatgttcag cggtctcccg ccggttcagc
      121 ggccaggaaa gaggttgtcc ggaaagcacc tcttcgagcg ttaaatctgc accataagga
      181 gcggatgggt tgcgtagtgc attgcggcgg ttcttaacca cgacagcggc gcactgctca
      241 ggagtggtgc ctgtttcgta taggtatcga ttcatctcca ttccagccac aaaatgggtg
      301 ttcaggcgca gtggtcgatt gagcaccggg tcctgtgcat aggcggttac ttctggaagg
      361 gtaagaatat tcgatgcttt gctatgtcct tcaatagcaa caagatcgaa catccctgta
      421 cgaatgagca tggcaccagt cgccaaacca tgcaagccat ctccggtaat tgtatgcact
      481 ggttttagcg ctgctccaat ctgatcaggt gtgtattcat cgaagatgct cgtcccctcg
      541 tgaaagtctt cggcaaccgt aacaaagctt tggacatctc tgcgtggatc aacgcctgca
      601 tcggaataag ctttaacggc tgcttcatag atcaattctt tataggatag atcgggtgtg
      661 atggggcgga aacctgccca tccaacgccg atgatcgcta cgcgatcatt catgcttgac
      721 ctcctttcaa aatttttccg gcagcgcgtg cccgctccaa gacacgctca gcggttttaa
      781 taatgggcgc gtcgaccatt ttcccttcaa gtgcaaatgc cccacgcccg gactgctgat
      841 gagctgcata cgcctccatg aggcgcaatg catgggatat agcctcgtca ctcggtgtaa
      901 atgctgattg aaccggctca acttgattgg ggtggataac ttgtttacct gaaaacccca
      961 ttcgagcgcc gagctggcat tcttgaacga gcccatcgac atcttgaaaa tcaagataaa
     1021 ccatgtcaat agcttgtaaa ttgaaggctg ctgcatgtgt tacgacagca ctgcgtgcat
     1081 aaaatacttc ccaaccctcg tggcttcgaa ttgctccaat gtcacctgcc aagtcttctg
     1141 cgccgaatat aagtgcctga aggcgcggat cggctgcggc aatgtgcggc agattgatta
     1201 ttcccgaggc ggtttctacg atggcaatga gaattatttc cccagctggc catccgaatt
     1261 tcttttctat cgaggcgatg gattggctag cccagcgaac ctggtcagag ttctcgacct
     1321 tgggaacgac aatcccatcg gggtgacctt ctaaacctgc aagcaagtcc tccagctcta
     1381 gcccagagcc cacagggttt atacggacaa gacgctccgc ttgcccaaaa tcgagcgtat
     1441 gtagcgcttc taaaatcgtc ttgcgcgcct cacctttccg attgaaggcg acgccatcct
     1501 caatatccat gcaaatgcag tccacgccca aggttgtggc cttgcggatt ttatgaaaat
     1561 cgtcacccgg catatagaga agggcgcgcc gcgcgcgcat gctcacctcc cgcgtcgagc
     1621 cagattagcc agaaaaatac taaggaaata caagacagtc atgggcgcca tcaccagggt
     1681 catattgaca ggatcaacag taggggtaat gctggcagca aggatagcaa ttactacaac
     1741 tgctagtcgc cattgatcga tgagcatctt gtgattgacg atcccgattg aagcaagcac
     1801 gtaaataacc aggggaaatt cgaaagatac accaatccag aacatcaggc tagtcacgaa
     1861 actgatatac gaagcggggc gtacttgcgg attcatcccg aggaaattta acaggaaggg
     1921 taaggctgtt gggagcatgg cgaagtaagc aaatgccatg cctgcgagaa aaaagattgc
     1981 gaccaaggga atagctatga gaccggtaaa tcgagagcgc cggtgcaggc ctggcgctgc
     2041 aaatagccat agctcaaagg caatataagg taaggcaagg gaaaatccta ccagcaaagc
     2101 gacacgcatg aacaccccaa ccggctcggt cacttcaata ggcgtgagtt ttccgattcc
     2161 accgattggc agggctagat aatcgataat ctggcgcgtg aaggcaaaag ccagggcagt
     2221 ggtcaaagcg aggaaaataa cagcgcgggt cagatgcttg cgaagtgcgt caacgtgctc
     2281 cagcaacccg gctggatttt caatggtttt tgctagggta tcagggaggg gctcatcctc
     2341 gacttctgct gtgaataatt gacgtatctc actggtgatg acagcaatcc atcgagcaat
     2401 tgtgcgaaat atccagataa cgaagcggaa tggagcggaa atgataagcc agatagccct
     2461 caggagggtg cgcatagtca atcttcggag ttaatttctt cgctttggat ggatggaggt
     2521 aatattttat tttcttcgtc aggcttagaa gagctattcg tttcaggtgg cgtggtccaa
     2581 tccgacaatc ctttacccac ctgggaaatg tctttagaaa cctcgttgat gccgattttg
     2641 cgagcgatct catctgtacg ggggagattt ttctgaattt cctccatacc agcttcacgc
     2701 atcaggcggt ttggaaggtg cctgatttcc tgcgaggttt gctggatggc gcgccaattg
     2761 ggtgaagtga caatactgcg tagtaggcgc ccgatggttt tccctgcctt aaccatatct
     2821 ttgggtccta atacgattaa tgcaatgatt acgacaaata ataattctag agggccaata
     2881 ccgagaaaat ccataaaata ttaacctgaa tccccaagat gattattatt attggggtga
     2941 ttttgggcaa gtcgattgaa agattgttga atctcgtgct ggcgacttgc caagctacct
     3001 aatacgccat tgacgaaacg tggagtgcta tctgaaccgt agactttagc cagctcgact
     3061 gcctcattga ttgcaacttt gactggtgtc ttgccgagca cggcgatttc ccatagagcg
     3121 atacgcagaa tattgcgatc aatagtagca acctggtcca ttggccattc aggtgcatgc
     3181 tcggcaataa actcatccag tgtagccatt aatgggaata cgccgaaaat gatctcgcgg
     3241 gcgaaatctg ccaagctgtc agccaagcct tcctcttcta cccgctgctc ccacacactt
     3301 ccaggggcat gaccagtgac atcaatttcg taaagtgctt gcaatgcaac gcttcgcgct
     3361 ctagtgcgcg gtttcatgag tgattaggcc tcgttctccc ttgcataatc aatgtcttcg
     3421 atgtgtacgt tcacactgcc cacctgcatg ccgaccatct cagagatcgc tcgcgtgact
     3481 tgttgctgca cattgcggct cacctcgcgc aagtttacat cacgattcaa aacgacatga
     3541 atatctgcaa aaactgtatc tcccctgact tcgatccgga taccttcatg aaaattgcgt
     3601 ttaaaaatcc ggtttaagcc ggggggatct atgcacatac gacttacccc gggtacattt
     3661 agggttgtaa gctgggcgat cgtaatcagc acttcagggg caattgtagt ttttccttcg
     3721 tccatggctc ctcataaaga ttacatcatt gccattccgc cgtcgacccc cagaatttgc
     3781 ccggttatgt aagctgcctg ttcagaggct aaaaatgcag cggcataggc aatttcctca
     3841 ggttggcctt tgcgacccag gggaataatg gtgagtgcgt tttggcgggc ttcttccggt
     3901 acattggccc atatttcggt atcaatatat ccagcagcta ttgcattgac tgtgatattc
     3961 ctcgatgcca cttcgcgtgc gagagctttt gtgaaaccaa tctgaccagc tttggaggca
     4021 gaataattgg tttgaccggc gtttcccatt tgaccggcaa cagaagtgat attgatgata
     4081 cgaccgtaac gcttgcgcat catatggcga actgctgcct tggagcaatt gaaggtgctc
     4141 tttaggtttg tgttttgtac acgatcccag tcttcttcgg tcatcatcat gattagcata
     4201 tcgcgagtaa tgcccgcatt gttaaccagg atatctaacc ccccaaaggt gtctattgcg
     4261 gtcttgataa gttcttgggc ttgctgaaaa tcggatacat ccgctctgac ggactttgcc
     4321 tgccctccac cagtcaggat ctgattgact atctcttcag cagccttttc tgaagtgttg
     4381 tagttgacta taaccgctgc attgagccgg gcaagttcca gggcaatcgc tcggccaatg
     4441 ccacgcgatg cgcctgtgac tactgcaacg cggccttcca aggatagccg ggctggttca
     4501 gacatcatac ctccgctaaa atatgtgctt atgtgtaacc gaggtattat accgtaatcg
     4561 gttcaaggta ttcacttgaa tgtcgtgatg aaataataaa gagttgtgtg ggtagaaatt
     4621 ccatccatag aaccctgagc tatcctgtcc taggatgaaa tgaggctacg gcaatgcgga
     4681 aatcaacttt tcaaaatctt caggggtccc aagcgtaagt gtttgaacct ggcggtcgat
     4741 gcgcttgatc aatccgccaa ggacggaccc gctcccgatt tcgataaacg tatcaattcc
     4801 atgcgagatc atatattgca ccgattcagt ccagcgaaca cgtgaagtga gctgtgcctg
     4861 gagatcgact ttgatgtgct ctgcgctggc aagcggtctg gcgtccacat ttccgataat
     4921 tggaatgacc ggatcttgaa ttggggagct atctaccgcg atattaaaat cctcttgggc
     4981 gttgatcatc aagggggagt gggcggcgat gctaatgggc agtggcacta cccgtcgtgc
     5041 tttcgcctcc tgagctaaac cgatagcgcg ctcaagggcg ctgctggccc cagaaatcac
     5101 cacctgccct gggcaattgt cattagccac ttgaacgacc tcatcctgtg tgctggcttg
     5161 tgcgcaaatc tcgtccaatg tgggaatgtc caaccccaat acagctgcca ttccacccgg
     5221 agatacaatt ccagcacgtt tcattaattc accacgccgg cgcacaaggt gcaaggcttc
     5281 ccgatagggg agcgcctcgg aggcgacaag cgcgctcagc tcacccatgg aatggcctgc
     5341 gacgtaagcg ggtcgaaact ctgagatgag ctctcggaat actctcagtg cagccactga
     5401 atggaccatc aacgcgggtt gagtattgat ggtatcgttt agtgtctcct cgggaccttc
     5461 ccaggcgaat tttgatagag gaaaaccaag ccattcgtcg gcaagctcgt atgcttctct
     5521 agcgactgta tatttatcgc aaaggagctg gcccattccc ataaattgcg atccctgtcc
     5581 tgggaagatg aaagcggttt tatttggttt cataggtata gataagttag tgcccgaatt
     5641 ttaggatcgt cgtgttataa atgctcagct caactcttcc ctggaactaa tacccccctt
     5701 cgcattaaaa ggggggatag tggaaatcta aagccagtga aaagcccaat attttgacca
     5761 gggcgagtca tttgtattat gatttcttct cttcaacttc gataatctcg cggcccttgt
     5821 aatgcccgca actagggcag accgtgtgtg gaaggcgcat ctcaccgcaa ttactgcagg
     5881 gcaccaaatt atgcgcttcc agggcatcgt gtgcgcggcg acgatcacga cgacctttcg
     5941 ataatttacg ctttggttga gggggcatgc tcttactcct aattaaatct ctttgcgaat
     6001 agactctcag tgtaaatcca gacgaatgcc tgccccttgg gcaggcaggg ggattataag
     6061 tttatgttgg gagaatgtca agatattata aaccaggttg gaatacgggg aacgaaaaga
     6121 ttgccataaa aaagtgcgag tgtaatatat aatatatttg tcacttcggt tttaacgagc
     6181 gggaggcatt atgaagcact ttaaagttca tgcaacaata gttatcgtcc tactttttgg
     6241 ggcagccgct tgcaatatac cggttggacc tcagccaatc atccctcagc aaacacctga
     6301 tttgaccatc acggctctat tcgagtttat taccacggcc caagctgagc aaacgaatgt
     6361 tgctgtacca acatctgctc cccctactga aacagcgtcc gtgacacccc ctccagtgca
     6421 ggcgacggca acgacaagcg ctccacccac accaacaaac acagctctgc caaccgatac
     6481 caaagtgcca accaaggttg tttcatcggg tggtagagaa cgcagtggat ctagcgtcat
     6541 tgcatattac atacaaaaag agccaacgat tgatggtgta tttgatgaat gggattttga
     6601 ccgctattcc gtttctcgcg tagtttatgg cgacaatcgc tggaatggga atgatgacct
     6661 atccgcaacc ctaatgattg gctgggatga ttattacctt taccttgctg tccgggtcaa
     6721 agacgatatt tacgtacaaa aagccagtgg caagaacctt tttaaaggtg atagcattga
     6781 gattttactg gacgctaatg tttccagaga ttataacgat aatgatctta gtaacgatga
     6841 ttaccagatt ggaatatctc cggggcaatc cacgcccagc gatgacatcg aaacctattt
     6901 atggtacccg tcctctaagg caggtgaata tacttccata aaggctgccg cattgccaac
     6961 agacgatgga taccgcattg aggcaaaaat tccttgggaa attttcggta ttacgccaga
     7021 tattggcaag catttcggat tcgccttttc tgtctcggat aatgacaaaa gcagtgaaaa
     7081 tgtgcagcaa tccatggtgt ctaacgtctc tacaaggcac ttgactgacc caacgacctg
     7141 gggtgaccta accttggagg gacgcagata acattttata tatgatgcat tcataacaaa
     7201 ggactaggtg gatgttcaac acccatctag tcctttgctt atgcgtattt tttgatttcg
     7261 gaaatgaatt actgtgagct aaaaaagcac ggaagccagc tcttgcctgt attggcgtgt
     7321 aactgggttc tcgtctccca tcaactcaaa taagcctaag attacttgtc gaacctcgcc
     7381 atcacgaaaa tacttgtcct ccctgaggat atccagaagt ccatctaggc ttgcggggat
     7441 attaccgcgc aagataagac gaagggcgcg ctgataagct gcctcaagcg gttgatcatg
     7501 attgttttcg ccattttgct ggcgtatgag cgcctgggta aggggtagca aggcttctgc
     7561 cctggcaaat tcgcggctag ggggcaagga ttgcaggatg cgttgcgctt catcaacctg
     7621 cccttgggcc aatatgctct tcgcaagacc gaacagggcg ggcgcggaat cagggttgac
     7681 cttgagtatt tcctggaatg cttcctgaga agatacccac tcgccctcag atatcaagct
     7741 taaacctttt tctagcaaca aatctgtgga gctaggggca agagcccgaa gccattcctt
     7801 cagctttggt tcaggttgca tcccgacaaa ttcagatacc attttgccat ctcggaatgc
     7861 tttcactgct ggtatgctgc gtacgttata ccgcaaagat aggttttgat tttgatccac
     7921 atttactttg gctaaacgga aagcgcctcc ggcttccagg gttaaacgct ccagcatagg
     7981 ccccagcgtg cgacagggga cacaccactc agcccaaaaa tccactacta cgggtatgtg
     8041 ctgagaatat gcgagtacct gatattcaaa atctgcttct gatacatcaa tgatattatc
     8101 tgaggccatc attttatgtt ctccatcatt aattcaccaa caattgcatt ttaacatccc
     8161 ttcgtaagag aattatatga aatctacata atttggtagg ggactttgcg aatttatcag
     8221 ggtgcttact caatgtcagg aggccctgct gaccctggtt gtacgacctc aaggacatca
     8281 ccgtggacgt taattcgtac tttgaatccc atcatgccga gataggcgcg aacatcttca
     8341 gggataccag tctcaatcaa ggagtccagc tgtttatcta tgttcttaat ctggttggcg
     8401 atcatggcga tggaaaagat atcttcctga ccaagcattt ttgctgcctg ctcactgatc
     8461 aaatcttcat gcccttgcat actctcgccc atcgcttcga gcacctggcg atccactagt
     8521 tctgaaggga tgtgtcgtaa aaaaccagcg tcatccacca cataacaggg ctcatccccc
     8581 acatctctca cctgtacgag gttgtcattc tcgtcaatga ctaaaccgga aaaaattgcc
     8641 tgacgggaca ttaggtcatg attcctggcg cccttcaatg attcccagca ggatagcagc
     8701 ggctagcccc acccgcctgt ccaacctgcg agctgaatcc atactaaagt ccagctccaa
     8761 ttcgtaacgg aaaaggttga agcgctggcg aaaatctgct aatctggttt caccagaaat
     8821 aacatcgtag ttttggggta atagagtacc cagcaataat cggcgcaata gggcgcgtgt
     8881 gggattgtcc tcttgcaaaa tggcctgagg ctgatcgctc gcgtccaata tctcccactg
     8941 gtcacgcaac aaggagcgca aacctttacg tctgagaacg cctactttga tgccggatag
     9001 actatcaatt acatcatagg ctgctgaaaa atcaataata ttgcgcgctt ggatgtagag
     9061 tagctcttgg ctgcggctct catcgttgta aactctaata tcctccctga ggcgaaacat
     9121 cttttgttgg gaatagagca acagggagcc atctggagag tacaagcgaa ttttcccagt
     9181 tagtgccaac acctgtcgct tgaggaagta ttggttgtag agaaatgctg aatgcatcat
     9241 ttaaatcaca tccacgattg tcccaaaatc tttgcggata aactgatccc taggtggcac
     9301 cataggcatc gtccagaggt aaatccactt ggcagccttt ggagatagat tgatgcagcc
     9361 atgactgcgg ggtcgaccgt aattgttgtg ccaatatgta ccgtggaacg agattccact
     9421 ctctgtgatg tagcaaatcc acggcactcc aggtaggtca taaccattat acgccagatt
     9481 cccagctgcc atgtgccgag atgggcgttt atgagcggtg atgtggcgcc ccggaggagt
     9541 ggcaaaattg ccgttgctaa attgtgctcc cgtggcagtc ttggccatga aaacaggctg
     9601 gtcccattcg taagcgatga ccacctgttc ttgcgtccgc acttcgatgc gcttggcaaa
     9661 tggtggtaag tcaggcgata ttgggttaag ctcctcagca ggtagtatgc gcaaatgggt
     9721 ggcaggcgca aaatacttta atttccactt atcttcggca attcgatacc acgcttgacc
     9781 caaattgtcg taaaccagac ctgttaccca atgggtggtt tcgtaatata gtcgataagc
     9841 tacggtatat tttttatccg gacgccagtg ggcatctgta tacgggaccg ttacctcagc
     9901 caccgttccg ccgtttggta tatctgcaag ctcagggttg agctcagtcg atactggctg
     9961 gatgcttcca gagtggacga agccttcgcc attaattgcg taccacacgc ggttgtgggc
    10021 aggttcgcta tctccaatgg ttgcttcgga gatcgggacg accatgtctt tccagaattt
    10081 ttgaagctct cttccgtgaa aagaaggaat atcatatagc tggatgccat cttccaggac
    10141 tcgaccctgc tgctccagag gtgttttttg gtcggcatgc tctcccggca agagaaaacc
    10201 caatactccc gcccccccaa gttttatgaa ttcacggcgg gagatagatc gggctatctt
    10261 cgcgtcctga ttctgccgcg tggtattcat gagtttactg gtggacgttg accaccgtgc
    10321 cgacggaggc aaagtcatat agccaaccgg cgtccttggt tttcaggttg acacagccgt
    10381 gactcattgg tcttccaaaa ttattatgcc agtaagtacc gtgcagcccg tagtccttgt
    10441 agaaatacat gacatacggc actttgggga gataataccc tggtcccgac atatcggcgg
    10501 cgcgatattt gacgtatatc ttgtaagtgc ccttcaccgt gggtgtttgc catgtaccgg
    10561 tagatactac aaatttattg acgagttgat cgccttgata gacataaacc cgttgcgagc
    10621 tcaaatcaat gtcgatccaa cgctcattcc cgtccacgcc acgaggtctt ccagggtaat
    10681 tgtaattttc tttggcaacg ttatttttct tgggtttttt ggtgggtttt ggtgtatcag
    10741 ttggcgtagg ggagggtgtt tccgttgggg ttggagaggg tgtaatggtg ggggtgggtg
    10801 tcgaagtcga agttggcgta ggagtaaatg tagcagtggg ggtcggggtt cgtgtttgct
    10861 tgcttagctg cacctgggct aggcttaatt gttttccatt gttgaacgcc aaggaaaaag
    10921 ttggcgatcc gaaccatatg gctaatccca gcaccagaag tgtaattgct atagcccagg
    10981 gcatgagcag agggcgctgg cgcgccattg aatctgaggt gattgttgct gcgacccggg
    11041 tacgtgggat aggggcaggt gcatccgccg ccgtattgtt tttgcgcagg cgttgaattg
    11101 cccaatgcat tccctttcgg gctcgctggg aagctggatt gatgcttaac gcgtgttgga
    11161 gatagttaac actgcctttt gggctggcta gagcccccaa tatcagccaa ccctcttccc
    11221 gttgaggctg aagcttgaca cacttttgtg ccaatcggcg agcttctaac cgatttccat
    11281 atctcagcgc gcgctttgcc tcactgagca gaacaccata agattcaaca agagaagtca
    11341 tggcgttggt gtggcgttaa cgataagatc ttcgttgttg atataacaga gaataccgtc
    11401 aaaaatgcct ttggccacca ggtcggattt ttgggtcagc aactcgcggt ctaaattgag
    11461 gaagcctatc tcgatgatcg cggcggtggt gttatcgcta atctcggcga aagcgtgata
    11521 gctggtcatg tcgatggtga tgctattgtg caatggaagt ccggtcacta tgccatagcg
    11581 attttttaaa caggctgtca ggcgggctgc acgatctgga tggggattgg ctaaagcaga
    11641 agataccttg aacccagtag cttgattatt tacataatca cacgagtcgg aatggattga
    11701 aaccagtacg gaggcttggt agtcgcttaa ttggggatca aactcattga gcacttcaac
    11761 gtcgtatcca ttgtctacca gatctttttg tacaagatag gctactttct cgttgacgct
    11821 tgcctctgtc agaccgtctg cgcagacgga acccgagtcg ttgcctttat gcccagcgac
    11881 gataccaatc agtggtttgc cgcgtggtgt tggtgtgatt ggggtttctg gggtaccggt
    11941 aacacctggg gcttgggcaa atgtgaagtt ttctgccagg tcactcggta acatgccagg
    12001 gtcggtccaa agcgtaaaga gtgtggcgag cacaaatgca atcccaacaa ccacctgaat
    12061 ataataaaaa gatcccctag atggtttagc gggtgaaacg ggctgtggcg gtgatgactc
    12121 tgtgctcatt gtgctccata gtaacttatt tgcatataga ttgcaagggt tttattgaga
    12181 ggagcaagaa ttatgccaat tttaaatgca aaattggacc agcgccgatg agcttcttat
    12241 ctttactgac ccaattctag ttgagtatgt ataggtttag taaaaccccc aggccgcgcc
    12301 agctccctct tccatcatgg agaaatccca catctctata cccatttcta tggtagtaga
    12361 gcgttgcagg gctttttcgc ctttttgacg ggtgatgtcc agtagtgctg gtccataggg
    12421 acaaaacgga gtggtgagga tcatggtgat ttttgcagaa tcttcttcaa ccaccacatc
    12481 acgcaccaaa ccaagctcaa tgatatttaa tccaatttct gggtcgacaa cttctcgtag
    12541 cgcatcacgc aagggagcgg ataattcggg atgggtgctg tcgacttgcc aaattagtgt
    12601 tgagctggtt tcctcactca ttgattattc tccaaagagg ccatactgga tactacatat
    12661 gtgcagttcg tatcgccatt caaaacgcat ttgacctttt ctgctggcac ggaaagcata
    12721 gtggaaatga gagtctggtc gacagagcaa acctcgggat gagattgccc aatatggtag
    12781 tacgggcaac tgatctcgcg tatatggtat tcgctgcctt tgcgttccca ttcgacggtg
    12841 aaaccttcac tgctgagcag ttgggcaacc aggtcaatcc gttcttccat gttgagcccg
    12901 ttgaccattg attcataatc agcggcaacg tccttagcca tttgccggaa aaggctgcta
    12961 accatcgctt ctggcatggt gtcttttagc tgttccagca accttatggt aaagcggagg
    13021 taacgtgatg gaaagcgctc caagcccttt tctgtaagga aatacaaccg tcgtggtcgt
    13081 ccaacaccat ggcgttcttc ttccgaattg actaaaccct ctgcttccag ccgggtgata
    13141 tgatgccgta ccgatatagg gttgatctca accgcttctg ccaggtcatt gattgtgcag
    13201 cgttgtctgc tgagtaatgt ctgcattaca cgctggcgag tgctcttaga tttttcttca
    13261 tctctaacta tggttgccat gcgaaaaagt atagcatgcg ggtcgaattc tgtcaatata
    13321 taagattttt atgctaaata ttattgactt ttgaagtacg ccttgctata atcgtgaata
    13381 tattagattt ttatgctaaa tattgacgga ttggcccagg actgctataa tactgcattt
    13441 ggatgggtgc attcaaatcg aattattatc acccctggtc gaatttgtaa tcacatacga
    13501 taaggagctt tatttatgtc tgaactttcc atccgcaatg tgcacgtgag catcgatggc
    13561 aaggaaatat tgaaaggtgt cgatctgacc ataagacagg gtgagatcca cgctttgatg
    13621 gggccaaatg gcactggcaa atctacgttg gcttatgctt taatgggtca cccaaactat
    13681 aaaatcactg acggtgaagt ctggttcaaa ggacaaaata tccttgatct ggaaccggac
    13741 cagcgttccc atcttgggat ctttctggcc tttcagtatc ccgtggcaat tccgggtgtt
    13801 tcagtggcta atttcttgcg ctcagcattg aatgctcggc gcaaggctga caatccagag
    13861 gacaaaggcg tcccgattcc cgaatttcgg aagctcttga aggaacgcat ggatctgctc
    13921 aagatggatc acagcttcgc tgggcggtat ctaaatgagg ggttttctgg tggggaaaag
    13981 aagcgcgctg aaatcttgca gatggccgcc ttacgccc
//
LOCUS       DBNGSX010000233         6487 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1681084, whole genome shotgun sequence.
ACCESSION   DBNGSX010000233 DBNGSX010000000
VERSION     DBNGSX010000233.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6487)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6487)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6487
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1681084"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="AC16CB_07100"
     CDS             <1..707
                     /locus_tag="AC16CB_07100"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR3342272.1"
                     /translation="DHNLKLVSHAPEFWDRTLVDLCSLDERQRCDSVKLMQKTIDLTR
                     EMAPHFAGTPKVVIHPGAMSLDHPIKDREKLYVNLRRSVNELDFEGVELLLENLPPHP
                     WYFGGQWLTNAFMDAREIRDFITSMGLNMCFDTSHAKLYCNWSHADFYEQVQILLPYI
                     RHLHLSDGAGLDGEGLQIGEGTIDWVHFFKVTGDYHGTMIPEIWRGHQRKGEGFLLAI
                     QRLSEAYFAARDNARG"
     gene            750..2402
                     /locus_tag="AC16CB_07105"
     CDS             750..2402
                     /locus_tag="AC16CB_07105"
                     /inference="COORDINATES: protein motif:HMM:NF017011.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide export protein,
                     LipB/KpsS family"
                     /protein_id="HIR3342273.1"
                     /translation="MPAQNSLLSRLSLGYAGWQNRARIAQLARQVAAHSPTSTQPSAD
                     PRPVVLFNASTRLTGLSLNAAFQLLTGWGLRLAGAPVVHFVCQSGLQPCVLGTNRQDY
                     TTPPPCDSCLAQSRRLYAVADVHGFGLKLDPQLSTALQNLSVDELGTFEYQLPALSDQ
                     HSPIPLGRLVVPSIRWALRRHNLPDDDPTRYLLRHYILSAYNVAVEFAALLDQLQPQS
                     ALIFNGAMYPEATARWVARQLEVRSVAHEVGFQRFSTFFTEGEPTAYPIHIPDDFELS
                     EAQNDQLDAYLEQRFQGKFSMAGIQFWPEMRGLDETLLQKTASFRQVVPVFTNVVYDT
                     SQVHANVIFPNMFAWLELVTEVIRAHPETLFVIRAHPDEMRPGTKKLSNESVHDWIHS
                     RGIHQWPNVVFIGPLEYLSSYELIHQAKFVIVYNSSIGLEATLLGVPVLCGGKARYTQ
                     YPTVFFPQSPEAFRQQANEFLKAESISVPIEYERNARRFLYYQLYRASIPLDDYLQTG
                     PRPGFVLLRDFSWRQLQPENSVPMRVLVEGILEEKPFLMPEN"
     gene            2406..3287
                     /locus_tag="AC16CB_07110"
     CDS             2406..3287
                     /locus_tag="AC16CB_07110"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3342274.1"
                     /translation="MEDKIEILSVELLDQHRPTVRALKRLAGSLGLEFGWHYLLDLTW
                     IISLLGAVKGKRILDAGAGTGVLQWYLAEQGAEVISVDRLSRANLPLRFRRRYNVRGL
                     RSQDLLPSATILRQSFTERNKLQLKVKSQVRDLAGLTDLRRVPGRVIIYNQELQNLSD
                     LADASIDAVVAVSALEHNPPEGLPGVVIELQRVLKPDGVLLATLGAARQADWLHVPSQ
                     GWCYTDASLRRHFDLPADTLTNYDRHDDLFSALRDCAELRDGLASFYYQSGDNGMPWG
                     RWDPQYQPVGVCKVKRG"
     gene            3368..3619
                     /locus_tag="AC16CB_07115"
     CDS             3368..3619
                     /locus_tag="AC16CB_07115"
                     /inference="COORDINATES: protein motif:HMM:NF012759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR3342275.1"
                     /translation="MNSYTTELTMSVCNLLASALQVPADSVSAELSFGDLPEWDSMGH
                     MEVMMQLEEHFGVEVSADTIAALTSVPAICSYLKENGHA"
     gene            3612..4799
                     /locus_tag="AC16CB_07120"
     CDS             3612..4799
                     /locus_tag="AC16CB_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class-II aminoacyl-tRNA synthetase family
                     protein"
                     /protein_id="HIR3342276.1"
                     /translation="MPETLEFTPTIPIPAHLVGEVQSKLAYIDEAVLGSEISPAGDRI
                     SLMLKKPVEGDRRAELEDKLQRVVVSMVKGAIRPKVQILEDHLDRRVPFNADPHDELL
                     ARRELSQEAHGIFALGPMISRLIAWFEEHFVGLADSFGAAPYRFPTLIPASYLERVDY
                     FSAFPHSLTFATHLRTDLDVIDHFAREASCGEGGELAAPPGSFAPVRALLSPAVCYHL
                     YFSLADQPLPGEGVIATSVGNCFRHEAINLTSLERLWNFTMREVIFVGSKEFVLDHRE
                     TARQRTAKFLGDIGLAYRVESANDPFFIGEFRKQVAFQSAFQLKYEIRARLPFKDSTL
                     AVGSYNYHQDFFGRRLNITLPDGSPTHTGCVAFGLERVAYAFLAQFGLDPRDWPDVVR
                     KDF"
     gene            4807..5310
                     /locus_tag="AC16CB_07125"
     CDS             4807..5310
                     /locus_tag="AC16CB_07125"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3342277.1"
                     /translation="MSAISYRPATESDFAALGAMYEKLNTFFYEVGYLLPRTENVGEL
                     WLDSFRRTLGRFSNVIIAEREGRPLGFILCRLKRLPAHMGGLLVGEVSDVWVEGEARR
                     ASVGEALVQAGVEWLRQQGVHSVEVQVLSENQASLKFFESLGFKLELRALRLILEKDA
                     QQAARFE"
     gene            complement(5389..6366)
                     /locus_tag="AC16CB_07130"
     CDS             complement(5389..6366)
                     /locus_tag="AC16CB_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006514625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HIR3342278.1"
                     /translation="MPEPFSPKPKKMLDQLREVIRIKHYSYSTEKNYVHWAKRYILFH
                     NKRHPAEMGAPEIEAFLSHLAQEVNVSASTQNQAFNAILFLYRNVLKIELTTPVYALR
                     AKRSQHLPTVLSKNEVNQVLGGMQGLHQLMARLLYGCGLRLMECLRLRVKDIDFEQCQ
                     IIVREGKGEKDRLTMLPVTVVEPLKAQIAFVQKQHMRDLEQGFGSVELPFALARKYPH
                     ADKELAWQYIFPSDRLSTDPRSGITRRHHLDPSGLQRAVKAAATLAKINKPISPHTFR
                     HCFATHLLEAGYDIRTVQKLLGHKDVKTTMIYTHVLKSGPKAVRSPLDE"
BASE COUNT         1357 a         1883 c         1797 g         1450 t
ORIGIN      
        1 gggatcacaa tctgaagctg gtatcacacg ccccagagtt ctgggatcgc acgctggtcg
       61 acctgtgctc gctggacgag cgccagcgct gtgactccgt gaagctgatg cagaagacca
      121 tcgacctgac gcgcgagatg gcgccccatt ttgccggcac acctaaagta gtgatacacc
      181 ctggagcgat gagcctggat cacccgatta aggatcgcga gaagctgtac gtaaacctgc
      241 gccgctctgt gaatgagcta gatttcgaag gggtcgaact gctattggag aacctgccgc
      301 cccatccctg gtacttcggc gggcagtggc ttaccaacgc tttcatggac gcccgagaaa
      361 ttcgtgactt catcacctct atgggcctga acatgtgttt cgacacctcc cacgccaagc
      421 tgtactgcaa ctggtctcac gccgattttt acgagcaggt gcaaatcctg ttaccgtata
      481 tacgccattt gcatctctca gacggagcgg gtctagatgg cgagggatta caaattggcg
      541 agggcaccat agattgggtg catttcttca aagtgaccgg tgattaccac ggcacaatga
      601 tccccgagat ctggcgcggc caccagcgca agggggaggg tttcttgctg gctattcaac
      661 ggctatctga ggcctacttc gcagctaggg acaatgcacg aggctgaacc aaagacacca
      721 gctacactgt taatttatcg accccaagca tgcctgctca aaacagtctt ctttctcgtc
      781 tcagcctggg ctatgccggc tggcagaacc gcgcccgcat agcccaacta gcgcgccagg
      841 tcgccgctca ttcccccacc tccacccagc catctgccga tccccgcccc gtggtcctgt
      901 tcaacgcctc cacgcggctg actggcttga gccttaacgc cgcattccag ctgctcactg
      961 gctggggttt gaggctggcg ggcgcaccgg tggtgcactt cgtctgccag tcggggctgc
     1021 aaccctgtgt tttgggcaca aaccgccagg actataccac accaccccca tgcgactcat
     1081 gcctagcgca atcccgccgt ctctatgcag tggccgatgt tcatggattt gggctgaagc
     1141 ttgacccgca gctctcgacc gctctgcaaa atctaagcgt agatgagctg ggcactttcg
     1201 aatatcaatt gccggcactc agcgatcagc actccccgat ccctttgggg cgcctggtcg
     1261 tcccttccat ccgttgggca ttacgccgcc acaatctacc agacgatgat cccacccgct
     1321 atctcctgcg ccactatatc ctctcagcat acaatgtggc tgtagagttc gcggccttgc
     1381 tcgaccaact tcaaccccag agtgccttga ttttcaatgg cgccatgtat cccgaagcca
     1441 ctgcgcgctg ggtggcacgg cagctggaag tgcgctccgt cgcccatgag gtgggtttcc
     1501 agcgcttctc cacctttttc accgagggcg agccgactgc ttaccccatt cacatcccag
     1561 acgattttga gctgagcgag gcgcaaaacg accagctgga cgcttacctg gagcagcgct
     1621 tccaaggcaa gttcagcatg gcaggcattc agttttggcc agagatgcgc ggcctggacg
     1681 agacattgct gcagaagacc gccagcttcc gccaggtagt gccggtgttt accaacgtgg
     1741 tctacgacac cagccaggtg cacgccaacg tgatcttccc aaacatgttc gcctggctgg
     1801 aattggtcac tgaggtgatc cgcgctcacc cagagacgct cttcgtaatc cgcgcccacc
     1861 ctgacgagat gcgccccggc accaagaaac tgagtaatga gagcgtgcac gattggatcc
     1921 acagccgtgg aatacaccaa tggcccaacg tggttttcat cggacctttg gaatacctca
     1981 gctcgtatga gctcattcac caggccaagt ttgtgatcgt ctacaactcg tccattgggc
     2041 tggaggcgac gctattgggc gttcctgtat tgtgcggcgg gaaagcgcgc tacacgcaat
     2101 accccaccgt tttctttccg caaagcccag aagcttttcg ccaacaggct aacgaatttt
     2161 tgaaggctga atcgatctct gtgccaatag aatatgagcg caacgcccgc aggttcctgt
     2221 attaccagct ctaccgggca tccatccctc tggatgacta tcttcagacc ggcccgcgcc
     2281 caggattcgt actactgcga gatttttcct ggcgccagct ccaacctgag aattcagtcc
     2341 cgatgcgagt cctggtagaa ggaatcctgg aggagaaacc atttttgatg cccgagaatt
     2401 agcccatgga agacaaaatc gaaattttgt ccgtagagtt attagaccaa caccgcccaa
     2461 cagtgcgcgc gctcaagcgc ctggcagggt ctttgggtct ggaatttggc tggcattacc
     2521 tgctagacct gacctggatc atctccctgc tgggggcagt caaaggcaag cgcatcttgg
     2581 atgcaggcgc cggcactggc gtcttgcagt ggtatctggc tgagcagggc gccgaagtga
     2641 tcagtgtgga ccgtctcagc cgcgcaaacc tgccgcttcg ttttcgcaga cgttacaatg
     2701 tgcgtggcct gcgcagccaa gacctgctcc ccagcgcaac gattttgcga cagtctttta
     2761 ctgagcgtaa taagctgcag ctcaaagtga aatcacaggt gcgcgaccta gccggactga
     2821 ccgatctccg ccgggtacca gggcgggtga tcatctacaa ccaggagttg cagaatttga
     2881 gcgatctggc ggacgcaagc attgacgcag tagtggccgt atcggcattg gagcataacc
     2941 ctccagaagg gctgcccggt gtggtcattg aattacagcg ggtgctcaag cctgatggcg
     3001 tactgctggc gaccctggga gccgcccgcc aagctgactg gctccatgtc ccttcccaag
     3061 ggtggtgcta caccgatgcc agcctgcgcc gccactttga cctgccagcg gacaccctca
     3121 caaactatga tcgccacgat gaccttttta gtgcactgcg agattgcgcc gagctgcgcg
     3181 acggcctggc gtctttctat taccagtcgg gggataacgg catgccctgg ggacgttggg
     3241 acccgcaata ccagccggtt ggagtttgca aagtgaagag gggataagga atttctgatt
     3301 gctgattgcc gatttctgat tgctgattgc tgatcactaa tcgctgatct actctcaagg
     3361 atgtttcatg aatagttata caaccgaatt gactatgagt gtctgcaatt tgcttgcttc
     3421 agccttacag gtgcccgcag actcggttag cgcagagctg agttttggcg acctgcccga
     3481 gtgggattcg atgggccaca tggaggtgat gatgcagctc gaagagcact ttggcgtgga
     3541 ggtcagcgcc gataccatcg ccgccctgac cagcgtgccg gcgatatgca gttatctcaa
     3601 ggagaacggc catgcctgag accctggagt ttacccctac catccccatc cctgctcacc
     3661 tggtgggtga ggtgcaatct aaactggctt atatcgacga ggctgtcctt ggcagcgaaa
     3721 tctcacccgc tggtgaccgc atctccttga tgctgaaaaa gcctgtggag ggagaccgcc
     3781 gggcagaatt agaagataag ctccagcgcg tggtggtctc gatggtcaag ggagccatcc
     3841 gccccaaggt gcagatcctg gaagaccacc tggaccgaag ggtgcctttc aacgctgacc
     3901 cccatgatga gctgctggcg cgcagggagc tcagccaaga agcccacggc atctttgccc
     3961 taggtcccat gatctctcgc ctgatcgcct ggttcgaaga gcacttcgtg gggttggccg
     4021 attcttttgg cgctgcgccc taccgctttc ctactttgat tccagccagc tacctggagc
     4081 gggtggacta cttcagcgct tttccccact cgctaacctt cgccacccac ctgcgcaccg
     4141 acctggatgt gatcgatcac ttcgcccggg aggcctcctg cggcgaaggc ggcgagctgg
     4201 cagccccgcc gggatccttt gctccggttc gcgccctgct ctccccggcg gtttgttacc
     4261 acctgtattt ttccctggcc gaccagccct tgcccggcga aggagtcatc gctacgtcgg
     4321 tgggcaactg cttccgccat gaagccatca acctgacctc ccttgagcgc ctgtggaatt
     4381 tcaccatgcg cgaggtgatt ttcgtcggct ccaaggagtt tgtgctagac catcgcgaga
     4441 ctgcccgcca gcgcacggca aaattcttgg gtgatatcgg cctggcctat cgcgtggaga
     4501 gcgccaacga tcctttcttc atcggcgagt tccgcaagca ggttgccttc cagagcgcct
     4561 tccagctcaa gtacgagatc cgcgcccgcc tgcctttcaa agactctacg cttgccgtgg
     4621 gctcatacaa ttaccaccag gatttcttcg gtcgccgcct gaatatcacc ctgcccgatg
     4681 gatcccctac ccataccggc tgcgtggctt tcggcctgga gcgcgtggct tacgccttcc
     4741 tggcccagtt tggcctggac ccgcgggatt ggccggacgt tgttcgaaag gacttctagt
     4801 tttgcgatga gcgctatatc ttaccgacca gccaccgaat ccgatttcgc cgccctcggc
     4861 gctatgtacg agaagctgaa tacttttttc tatgaggtgg gctacctgct gccccgcaca
     4921 gagaatgtgg gcgagctttg gctggattct ttccgccgca ctctagggcg cttcagcaat
     4981 gtcatcatcg ctgaacggga aggtcgtcca ctgggattca tcttgtgccg tctaaagcgc
     5041 ttgccagcgc atatgggtgg cctgctggta ggcgaggtga gtgatgtctg ggtggagggc
     5101 gaggcgcgcc gggccagcgt gggcgaagcg cttgtgcagg ctggagtgga gtggctgcgc
     5161 cagcagggcg tgcactccgt ggaagtccag gtgctcagcg aaaaccaggc tagcctgaag
     5221 ttcttcgaga gcctggggtt caagctcgag ctgcgcgcgc tccggctgat cctggaaaaa
     5281 gatgctcagc aggcagcacg atttgagtag gatctggcac agctagacaa agcccgtaca
     5341 aggattaaaa ccccagacaa gagcagggac aaccttgaaa aagcagcctc actcatccag
     5401 tggactacgc accgccttcg gcccgctctt gagtacatgg gtgtaaatca tcgtcgtttt
     5461 gacatccttg tggccgagta gtttctgcac agtgcggata tcatagcctg cttcgagcag
     5521 gtgggtggca aagcagtgac ggaaggtatg cgggctgatt ggcttgttaa tttttgccag
     5581 ggttgcagcc gccttcaccg cccgctgcaa cccgctggga tccaaatggt ggcggcgggt
     5641 aatcccactg cgcggatccg tggacaggcg atcagaggga aagatatact gccaggccaa
     5701 ttccttatct gcatgcgggt atttgcgcgc caaggcaaaa ggcagttcca ccgaaccaaa
     5761 tccttgctct aaatcacgca tgtgctgctt ctgcacaaaa gcaatctgag ctttcagcgg
     5821 ctccaccacg gtgacgggca gcatcgtcaa gcgatctttc tcgcccttgc cttcgcgaac
     5881 aatgatttgg cattgctcga agtcaatgtc cttgacccgc aggcgcaggc attccatcaa
     5941 gcgcaaaccg cagccataca acaggcgagc catgagctgg tgcagccctt gcatcccgcc
     6001 caacacctgg ttcacctcat tcttggaaag cacggtcggc aagtgttggc tgcgtttggc
     6061 gcgcaaggca tagaccgggg tcgtcagttc aattttcaat acgttgcgat agaggaaaag
     6121 aatggcatta aaagcctggt tctgggtgga tgccgagaca ttgacttcct gggccaggtg
     6181 ggaaaggaat gcttcgatct ccggcgcgcc catttcggca gggtggcgct tgttatgaaa
     6241 gagaatatag cgctttgccc aatggacata gtttttttcg gtagaatacg aataatgctt
     6301 gatgcggatg acttcgcgta gttggtccag catttttttg ggctttggcg aaaatggctc
     6361 aggcataagc gactcgctta tgaggggggg ataatattta tatttgtgtt gcagaaatca
     6421 catgataatt cgagtacgag ttaaccggca ataatgcagt atatctagac tgaaaacata
     6481 tattata
//
LOCUS       DBNGSX010000234         4180 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1611492, whole genome shotgun sequence.
ACCESSION   DBNGSX010000234 DBNGSX010000000
VERSION     DBNGSX010000234.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1611492"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2192)
                     /gene="lon"
                     /locus_tag="AC16CB_07135"
     CDS             complement(<1..2192)
                     /gene="lon"
                     /locus_tag="AC16CB_07135"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR3342279.1"
                     /translation="MRNHDWFTRNDDDDEFGEALHRRTEELYDVPDAIPDEDGLIECP
                     VLPLRDLVVFPRMVSPIFIGREASLLAIEEAQMKDQTLIALVQRDPEQDDPGPSDFLG
                     VGVELAVGRLLNMPDGSNSALVQGRRRVELIEFTQLEPYLRARTRPVYESVDVDRQTD
                     ATMRTVLELFQKCVNLDRSLPEEAYLYALNVEEPGWLADMIVTAIAPPLEERQTILES
                     VDPLERLRQVIKLLAREVDVLELEDEIHTRAQSEVDRTQREYYLREQMKAIQTELGEG
                     DLWSRDIQELRTRLESANLPEEVQVRAIKELDRLGQMPPMSPEVGILRTYLEWILELP
                     WTHVTEDNLDVKHAAKVLERDHFGLPRAKERVLEYIAVRSLKPKRMRQPILCFVGPPG
                     TGKTSLGRSISEALGRKFVRLSLGGVRDEAEIRGHRRTYIGALPGRILQTMRRAGTAN
                     PLFMLDEIDKLGQDFRGDPAAAMLEVLDPEQNHAFSDHYLELPYDLSKVMFITTANTV
                     GTIPPALLDRMEIIEFPGYIEEEKLEIATRFLIPRQFEESGLDSGELRLSTQALQRII
                     REYTYEAGVRNLEREIGRICRKIARLKAEGKRHPNQVSANVIERYLGPPQFFNMEAEL
                     KNEVGVATAVAWTENGGEIMPVEVLLMDGKGNLQITGQIGNVMQESAQAALSYLKSRA
                     KALDLDAEIFDQVDVHIHIPEGAIPKDGPSAGITIGTALVSAFTGREV"
     gene            complement(2192..3535)
                     /locus_tag="AC16CB_07140"
     CDS             complement(2192..3535)
                     /locus_tag="AC16CB_07140"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR3342280.1"
                     /translation="MTTEDQYQTTLDYLYSFVDFSLTKGMRYTPEQFDLGRMRAFVQT
                     LGNPERAYPSIHVAGTKGKGSVSSMCSAVLQAEGYRVGLYTSPHLHDYAERIRINQVS
                     IPHQDLISLVEEIKPVIEAVPGVSTFEITTALAFLYFQHQKVDVAVLEVGLGGRLDAT
                     NVVEPSLAVITSLSFDHTQILGNTLSEIAREKAGIIKAGIPVVVSPQDDEARKVIEQI
                     ALERSAPLIQVGRDYSYAALHHSLEGQSLQVWNGTEKRDAEPLAIPLLGMHQVENAAT
                     AYAALQVFSQRALPVSLGSIKRGFTQLTWPGRFEILQRQPPVVVDSAHNRDSAKRLRQ
                     ALDDYFPDMPVVMVFGASEDKDIAGMFAELSPRVQEVVATKSIHPRAIEPEHVVEIAH
                     QNGIPAKIVADIPEAVMVALELADGDKLTLVTGSIFVVAATREAWMARAVNLDNG"
     gene            3633..>4180
                     /locus_tag="AC16CB_07145"
     CDS             3633..>4180
                     /locus_tag="AC16CB_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SanA/YdcF family protein"
                     /protein_id="HIR3342281.1"
                     /translation="MLLRIILLLASAGILTLLIPRIVTALYALPRTYHAEDVPFKPIA
                     IVYGAGLWWDGTPSPVLRDRVATAAQLYFTGKVNKLLMSGDNRTEFYNEPGAMREYAL
                     GLGVPEEDIVLDYAGQRTYDTCYRAREIFGIKEATLVTQGFHLPRALYTCNQLGVEAI
                     GVPADLRDYRRGARIFWHGREVP"
BASE COUNT          952 a         1199 c         1040 g          989 t
ORIGIN      
        1 aatacctcac gcccggtgaa ggccgatacc agtgcagtgc cgatcgtgat tccagcgctt
       61 ggtccgtctt tgggtatcgc cccctccggg atgtgaatat gcacgtccac ctggtcgaat
      121 atctccgcat ccagatcaag agctttcgca cgcgacttca aatatgaaag cgcagcctgg
      181 gccgattcct gcatcacgtt accgatctgt cctgtgatct gcaggttacc cttcccatcc
      241 atcagcagca cctcaactgg catgatctct ccgccatttt ccgtccaggc tactgcggtt
      301 gccacaccca cttcgttttt cagctcggcc tccatgttaa aaaactgtgg gggtccaagg
      361 tagcgttcaa ttacgttggc gctcacttga ttggggtgac gcttaccttc cgccttcaat
      421 cgagcgatct tgcgacagat tcgcccaatc tcccgctcta gattgcgcac ccctgcttca
      481 tacgtatact ctctgataat ccgctgtaac gcctgagtag acagacgcag ctccccggaa
      541 tccaaaccgc tctcttcaaa ctgtcgcggt atgagaaagc gtgttgcgat ctccagcttc
      601 tcctcttcaa tataacctgg aaattcgata atctccatgc ggtctaagag tgccggtgga
      661 atcgtgccta cggtattggc agtagtgatg aacattacct tggaaaggtc atatggcagc
      721 tcaaggtaat ggtctgaaaa ggcgtggttt tgctccggat ccagaacttc cagcatggct
      781 gccgccgggt cgccacgaaa atcctgaccc agcttatcaa tctcatccaa catgaaaagg
      841 ggattcgccg tgcccgcgcg ccgcatagtc tgtaagatac gtccggggag cgcaccaata
      901 taggtacgac gatggccgcg tatctcggcc tcgtcacgca cgccgcccag ggaaaggcga
      961 acgaacttgc ggcccagcgc ctcggaaatt gagcgcccca aggatgtctt gcctgtaccg
     1021 ggcgggccaa caaagcagag gatgggttgg cgcattcgct tgggtttcag gctgcgcact
     1081 gcgatgtatt ccagcacccg ctctttggca cgcggcaggc caaaatggtc tctttcaagc
     1141 actttggcag catgttttac atccagatta tcctcggtca catgcgtcca ggggagttcc
     1201 aaaatccact ccaggtaggt gcgcaagatt cccacctcag gcgacatggg gggcatctgt
     1261 cccaaacgat cgagctcttt gatggctcgc acctggactt cctcgggcaa attagccgac
     1321 tctaatcggg tgcgcagttc ttgaatatcg cgcgaccaga gatccccctc ccccaattct
     1381 gtctgaatag ccttcatctg ctcacgcagg tagtattcac gctgtgtgcg atctacctcg
     1441 ctttgggcgc gggtgtgaat ttcatcttcc agctccaaga catcaacttc tctcgccagc
     1501 agcttgatca cctgtctaag gcgctcgagc gggtcaacag actctagtat cgtttgtcgt
     1561 tcttccaggg gaggggcgat cgcagtaaca atcatatcag ccagccagcc aggttcttca
     1621 acattcaatg catacaggta ggcttcttct gggaggctgc gatctagatt gacgcacttc
     1681 tgaaacaact ccaaaacagt gcgcatggta gcgtcagtct ggcgatccac atcgaccgat
     1741 tcgtagactg gtcgcgtgcg ggcgcgcagg taaggttcca attgagtgaa ctcgatcagc
     1801 tccacccgcc tgcggccttg caccagcgcc gaatttgagc catccggcat attaagcaac
     1861 cgaccaacag ccaattccac gccaacgccc aaaaaatccg atgggcctgg atcgtcctgc
     1921 tcaggatcgc gttgcaccaa ggcgatcaac gtctgatcct tcatctgggc ttcttcaata
     1981 gccagcaagg aagcctcgcg tccgataaaa atcggggaaa ccatacgcgg aaatacgacc
     2041 aggtcgcgca aaggcagcac tggacactct atcagcccat cttcatccgg tatggcatcc
     2101 ggcacgtcat aaagctcctc ggtacgccgg tgtagcgctt cgccgaactc atcatcatcg
     2161 tcattacgtg taaaccaatc gtgatttctc attatccatt atccaaattt acggcgcgcg
     2221 ccatccaggc ctcgcgcgta gccgctacga caaaaatact gccggtgacc agggttaatt
     2281 tgtcaccatc ggctagttct aatgctacca tcactgcttc tgggatatcc gctacgattt
     2341 tcgctggaat accattttgg tgtgcaatct ctaccacatg ctcaggctcg atggcgcgag
     2401 gatgaatcga cttggtcgca accacctctt gaacccgcgg actcagctca gcgaacatac
     2461 ccgcaatgtc tttatcttct gaagcgccga acaccatgac aacaggcatg tctgggaagt
     2521 aatcatctaa tgcctgccgc aacctctttg ccgaatcgcg gttatgtgcg gaatctacca
     2581 caacaggcgg ttgccgctgg agaatctcaa agcggccggg ccatgtcagc tgagtaaagc
     2641 ctcgcttgat cgagcctaga ctcactggca acgccctctg agagaacacc tggagagccg
     2701 cataagcagt tgcagcattc tcaacctggt gcatgcctag cagaggaata gccaggggtt
     2761 cggcatctct cttttctgtc ccattccaca cctgcaggga ttgcccttcc agggaatgat
     2821 gtaatgcggc ataggaataa tctcgaccaa cctgtattaa cggagcagat cgctctaatg
     2881 caatttgttc gattactttc cgggcttcgt cgtcttgcgg agagacgact accgggatgc
     2941 ctgccttgat gatcccagcc ttctcgcgtg cgatctcgga gagcgtattt cctagaattt
     3001 gcgtgtgatc aaaggagagc gacgtgatca cagccaagga cggttccact acgttggtgg
     3061 catccagccg accgcccaag ccgacttcta gtacggcgac atcaaccttc tggtgttgaa
     3121 aataaagaaa agccagcgca gtcgtgattt caaatgtgga cactccaggc actgcttcga
     3181 tgaccggctt gatctcttca accagcgaaa tcaaatcctg gtggggtatt gagacctggt
     3241 tgatgcggat gcgctcggca taatcgtgca ggtgcggtga ggtatacaga cctacccggt
     3301 aaccttcagc ttgtagcact gcactacaca tggatgacac agaccctttc ccttttgttc
     3361 cagccacgtg gatgctaggg taagctcgct ccggattacc cagagtctga acgaaggcgc
     3421 gcatacgccc aaggtcaaac tgctccgggg tatagcgcat accttttgtc aggctaaaat
     3481 cgacaaagct ataaaggtaa tcaagtgttg tctggtattg atcttctgta gtcatcgtgg
     3541 tgggattgta accgtatgat ccctagtgtg caagaggaag tacctcgatt taattcgcgt
     3601 gatacaattc gcccgtgttg cgatttgcaa aaatgttact ccgcatcatc cttctattgg
     3661 cctcagccgg cattctgact ctgctcatcc ctcgtatagt taccgcgcta tacgctttgc
     3721 cgcgcacata ccatgccgaa gatgtccctt ttaaacccat tgccatcgtc tatggagcag
     3781 gtctatggtg ggatggaact ccctctccag tgctgcgcga tcgtgttgcc acagcagctc
     3841 agctttactt taccggcaag gtcaataaac tattgatgag cggtgacaac cgtacagagt
     3901 tttacaacga gccaggggcg atgcgcgaat atgccctcgg actgggtgta cccgaagaag
     3961 acatcgtctt ggattacgcc ggccaacgca cttacgacac ctgttatcgc gctagggaaa
     4021 ttttcggcat aaaagaagct actcttgtca cgcaaggctt tcacctgccg cgcgccttgt
     4081 acacatgcaa ccagctcggt gtcgaagcca tcggagtgcc tgcagatctg cgagactacc
     4141 gtcgtggcgc gaggattttc tggcatggcc gcgaggtccc
//
LOCUS       DBNGSX010000235        10046 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_434431, whole genome shotgun sequence.
ACCESSION   DBNGSX010000235 DBNGSX010000000
VERSION     DBNGSX010000235.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10046)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10046)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10046
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_434431"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1973)
                     /gene="topA"
                     /locus_tag="AC16CB_07150"
     CDS             complement(<1..1973)
                     /gene="topA"
                     /locus_tag="AC16CB_07150"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIR3342282.1"
                     /translation="MEVIILEAYCVKCKTKRELSDPQPVFTKGGTPATRGVCPVCGTN
                     MVRMGRTPAHEGLTPDETLVKPRQKRSGKLVIVESPAKARTVGRFLGKGYTVKASIGH
                     VRDLLKSQLSVDVENDFTPKYRVPKEKRQVVNEIKDLVKKAEKIYLATDPDREGEAIA
                     WHLMESARIDPEITQRVVFHEITEPAVAEAFSHPRGINMDLVNAQQARRVLDRLVGYN
                     LSPLLWRKVRSRLSAGRVQSVALRLVVEREREIDAFVPEEYWTIAGEFLPEGGKETFI
                     AKLAKIDKQEPVLSKEADVQPVLAEMQSAVYTTSKVKRGERRRSPAAPYTTSTLQQEA
                     SRRLGYTARRTMAIAQQLYEGVDVGEGGSVGLITYMRTDSTSVSELARSEARNFIAGR
                     FGETFLPKQAPRYKTKARGAQEAHEAIRPTAVNRQPNSIKDYLTRDQFRLYQLIWQRF
                     LASQMAAAIFDTLTVEITGLAQHEYLLRASGSTVRFPGFLVVYKEARDEDIKTEEEGE
                     TRLPSNLVEGQSQRLVRLLPEQHFTQPPPRFTEATLVRTLEEYGIGRPSTYAPILSTL
                     QSRGYVLRESKRLSPTETGNLVNNMITEYFPNIVDVGFTARMEEDLDRIASGVQPWVD
                     PIKQFYTPFSRQVADADARIPEMKKELEPVGRD"
     gene            complement(2006..3094)
                     /locus_tag="AC16CB_07155"
     CDS             complement(2006..3094)
                     /locus_tag="AC16CB_07155"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004475 - mannose-1-phosphate
                     guanylyltransferase (GTP) activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HIR3342283.1"
                     /translation="MFDHFYAIIMAGGGGTRLWPLSRQAKPKQFLRLIEERSLFQTSV
                     QRLEGLFPPERIYVVTVAEQAAELQLQSPAIPKENFLLEPMPRGTASVVGLAAVALQQ
                     RDPQAVMAILTSDHFIGNEEGFRKMLAAAHNAAQDNHLVTLGITPSYPATAYGYIQRG
                     ERITTYRNYDAFRVARFREKPDEARARRMLKQGDHAWNSGMFVWRVDRVLSEFATQMP
                     KLYSGLQEIQQVWGSSQQQTVIYRIWDQLVSETIDYGIMEGAKDVAVIMADDLRWNDV
                     GSWDSLFDVLPSDPQGNIVMGGEHIGLDTRETLVYVNQEHRLIVTIGVQDLVLVDTGD
                     VLLVCRKDQAQKVRQIVKSLKESGRSYI"
     gene            complement(3105..4217)
                     /gene="dprA"
                     /locus_tag="AC16CB_07160"
     CDS             complement(3105..4217)
                     /gene="dprA"
                     /locus_tag="AC16CB_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR3342284.1"
                     /translation="MIDPKAYWVGFNLVKGIGAVRLRNLIQHFGDVETAWHASPQEMR
                     AAGLGPKLIETMQQVRAGVSLELVWERVHSLGIQVLTWEDEAYPRRLKEIDQPPPVLY
                     LRDNLQPEDEWAVAIVGTRGITAYGRQVTAEIASVLARRGVTVISGLARGVDAEAHKA
                     ALNAGGRTLAVLGSGVDQIYPPEHRQLADQIIASGALMSDYAPGTPPDGVNFPPRNRI
                     ISGLSMAVIVIEAGERSGALITAEFAADQGREVFAVPGNINAPKSVGCNRLIHQGARP
                     LLTPEDVLESLDLASFQQQRVARAVLPKDEVEARLLSVLTREPMHVDDIRARVDMPIE
                     KVSATLALMELKGMVRQLGGMNFVAIREEAEDYIIK"
     gene            complement(4318..4872)
                     /gene="rimM"
                     /locus_tag="AC16CB_07165"
     CDS             complement(4318..4872)
                     /gene="rimM"
                     /locus_tag="AC16CB_07165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR3342285.1"
                     /translation="MTFSEPSSEQTTGLPPSGEPVFLVVGRLRRPHGVSGEILMDVIT
                     DFPERMRSGMTVYVGENHLPLQIRKCRPHDKNLLLAFKEILDRDAVGALRNKLLYVRA
                     DDIPALPEGDYYHHQILGLRVYSEDGEFLGTVVDILETGANDVCIVRPSEGSDFLMPV
                     IDSIVLKIDLDQGEMRVRLLEGLR"
     gene            complement(4869..5108)
                     /locus_tag="AC16CB_07170"
     CDS             complement(4869..5108)
                     /locus_tag="AC16CB_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HIR3342286.1"
                     /translation="MVKELISYIATSLVDDPTQVQVSQFQRGPSMSLRLKVAKEDMGR
                     DIGKGGRVANAMRILLRVAAAQEGKRASLDVDEPR"
     gene            complement(5206..5547)
                     /gene="rpsP"
                     /locus_tag="AC16CB_07175"
     CDS             complement(5206..5547)
                     /gene="rpsP"
                     /locus_tag="AC16CB_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR3342287.1"
                     /translation="MVKIRLRRMGAKGQPTYRIVAADKESPRDGRFLEILGSYNPRTV
                     PATILVKEDRVYHWMSQGAQPSDSVARLFRTTGLLDRYARYKQGEELETLLAEAAAEA
                     EANPISPKTRQ"
     gene            complement(5655..6977)
                     /gene="ffh"
                     /locus_tag="AC16CB_07180"
     CDS             complement(5655..6977)
                     /gene="ffh"
                     /locus_tag="AC16CB_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR3342288.1"
                     /translation="MFENLTERLENVFKQLRRRGKLSESDVDAAMREVRLALLEADVN
                     YAVVKDFVARVRQRAIGYEVSKALNPGQQVVKIVNEELIATLGAPDRFDLSGPKPRTI
                     MLVGLQGSGKTTAAGKLARLLRAQGERVMLVACDPYRPAAVRQLETLGERLNVPVHFE
                     EGIQPPQLAVRARDKAQKGGFTVLILDTAGRSQLDDALMNELQEIRRRVDPVETLLVV
                     DAMIGQEAVNIAKGFRDVVTLTGLLLTKMDGDARGGAAISIRSVTGVPIKFLGTGEAL
                     DAIEAYDPARLSSRILGMGDMIGLIEKAEAAFDEKVAAEQANRMLSGEFTLQDFADQL
                     SQLRKMGPLGQLMDMLPGGMGQMARQINPQDAERQFKRTEAIIGSMTKDERRKPDILN
                     ASRRRRIASGSGTHVQDVNRLIKQYRDAQRLFKSLKKSGKRGIPRLFG"
     gene            7135..7524
                     /locus_tag="AC16CB_07185"
     CDS             7135..7524
                     /locus_tag="AC16CB_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3342289.1"
                     /translation="MLQDKSSPVIDDPAKLFKILMHPARLSILDILRDSEHCVCHMES
                     HLGYRQAYISQQLMVLREAGLVEDRKDGLNVFYRVTVPTLYEVIDVARKITYVDEQIQ
                     EHDPHHEAKAASCPCPKCNPAAKNVSC"
     gene            7640..7903
                     /locus_tag="AC16CB_07190"
     CDS             7640..7903
                     /locus_tag="AC16CB_07190"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024590.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR3342290.1"
                     /translation="MLEIKILGTGCAGCLKTEQVVHEALRALGIQNANVELVAEQRMI
                     EYGLLADRAPGLLINGYLAWAGTVPTIEQVMEWVTQAVTPTVI"
     gene            7965..8405
                     /locus_tag="AC16CB_07195"
     CDS             7965..8405
                     /locus_tag="AC16CB_07195"
                     /EC_number="1.4.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF013743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HIR3342291.1"
                     /translation="MTDFLQITVDKFTFKVATDRYYNEEGVWVLAQGNRVRIGLSDFL
                     QQRSGDVAFAEITPQGTVLAVGDEIAVIETVKVNISLSSPVSGSIVEINPLMETAPEI
                     INQDPYGEGWLAIIEATQWESDSSRLLDPQAYYSRMQIQAEESV"
     gene            8408..8833
                     /locus_tag="AC16CB_07200"
     CDS             8408..8833
                     /locus_tag="AC16CB_07200"
                     /inference="COORDINATES: protein motif:HMM:NF020434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HIR3342292.1"
                     /translation="MSQVSVIIVPCSGIGKTYGTVSREAAYEITEELRPDQTQLVPLS
                     LLVLGDEATHSTVMGNPAITIDGCKLACATKMVKECGGQVAHNIAVLDVYRRHKQFKP
                     QGIAILNEGGLKLAHALAEEILPMADQLMEIARKREPYG"
     gene            8826..9554
                     /locus_tag="AC16CB_07205"
     CDS             8826..9554
                     /locus_tag="AC16CB_07205"
                     /inference="COORDINATES: protein motif:HMM:NF020434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HIR3342293.1"
                     /translation="MVDLPKKKVGIIACSGEELPEGTVTRLAALRVLEELRPSDTVTI
                     CLPLFLAGGEGDRAFARFYPTIALDGCEKRCAARGTEMYSGKPAASVVVSELIHQNSL
                     PQPRGRRKLDEAGRYIVNFSAEHVANLVDDLLDKRWNRQAGKFAEETHPAASSLQTTC
                     TCGSDIPSQAIFIEGQAVTIIALPLIFQQLHEAGKIIRQDNARELLELVRVYNPIPNR
                     AEEAYTSALLREYHTYCKQKATSQ"
     gene            9551..10003
                     /locus_tag="AC16CB_07210"
     CDS             9551..10003
                     /locus_tag="AC16CB_07210"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR3342294.1"
                     /translation="MSRTETYHTTVTWKNGHWGHIAMGNGAEMDFSAPAEAHGHPGVL
                     TPEDAFVAAANTCIMMMFLWTAERFKLKLLSYECRAEGTKLIELDRTEIFTHLHLWPV
                     ISISAAGEDRGVINARAQRAMQSAQKYSLVANSIKSEIVIQPKIEVIE"
BASE COUNT         2408 a         2779 c         2524 g         2335 t
ORIGIN      
        1 cagtcccttc caactggctc cagttccttc ttcatctctg gaatgcgggc atcggcgtca
       61 gccacctgcc gggagaatgg cgtgtaaaac tgcttaatcg gatccaccca gggttgcact
      121 ccggaggcga tgcgatccag atcctcctcc atgcgcgccg taaaccctac gtcgacaata
      181 ttagggaagt attcagtgat catattattg accaggttgc ccgtctctgt ggggctaagg
      241 cgcttgctct cgcgcaagac atagccacga ctttgcaagg tagataagat cggtgcgtaa
      301 gtcgaaggtc gcccgatacc atattcctcc agtgtgcgga ccagcgttgc ctcggtaaag
      361 cgcggtgggg gttgggtgaa gtgctgttct ggcaacaatc gcactaggcg ctgagactga
      421 ccttctacta ggttggatgg gaggcgcgtt tcaccttctt cttcggtctt tatgtcttcg
      481 tcccgggctt ctttatagac aaccaggaaa cccgggaaac gcactgtgga accggaggcg
      541 cgcagcagat actcgtgctg cgccaggccg gtgatttcca cggtgagcgt atcgaaaata
      601 gccgctgcca tttgggaggc caggaagcgc tgccagatca gctggtatag acggaattga
      661 tcacgagtga ggtagtcctt tatcgaattt ggctgtcggt tcactgcggt agggcggatt
      721 gcctcatggg cttcctgtgc accgcgcgct ttggttttgt agcgcggagc ctgcttaggc
      781 aggaaagttt cgccaaagcg cccagcgatg aaattgcgtg cctccgaccg cgccagttcc
      841 gaaacactgg tcgaatcggt gcgcatatag gtgatcaggc cgactgagcc cccctcaccc
      901 acatccacgc cttcgtagag ctgttgagcg atcgccattg tgcgcctagc tgtataacct
      961 aatctgcggg aagcctcctg ctgcagtgtg ctggtagtat acggcgccgc cgggctgcgc
     1021 cgccgctcgc cacgcttgac cttgctggta gtgtagacag ccgactgcat ttccgccagg
     1081 acgggctgca cgtcggcttc cttgctcagc actggctcct gtttatcgat cttagccagc
     1141 ttggcgataa aggtttcttt accgccctcc gggagaaact cgcccgcgat ggtccaatat
     1201 tcctcaggga cgaaggcatc gatctcacgc tctcgctcga caaccaggcg cagcgctacg
     1261 gactggacac gaccggcgga gagccggctg cgcaccttgc gccacagcag cggactgagg
     1321 ttatagccta ccaatcgatc caatacccgg cgcgcttgct gagcgttcac caaatccatg
     1381 ttgatgccgc gcgggtgaga aaaagcttcg gcaaccgccg gttcggtaat ctcgtgaaac
     1441 accacgcgtt gggtgatctc agggtcaatc ctagccgact ccatcaggtg ccaggcgatt
     1501 gcctcaccct ctctgtcggg gtcggtcgcc agatatatct tctccgcttt cttaaccaga
     1561 tctttgatct cgtttaccac ctggcgtttt tccttgggca cacggtattt gggggtaaag
     1621 tcgttctcta catctaccga aagctgcgat ttgagcaaat cccgcacatg ccctatggac
     1681 gccttgaccg tgtagccctt gcccaggaag cgacccacgg tacgtgcctt ggctggcgac
     1741 tcgacaatga ccaatttgcc agagcgtttc tgcctgggct tgacaagcgt ctcgtcgggt
     1801 gtcagtccct cgtgggcagg tgtgcgcccc atgcgcacca tattcgtgcc gcacaccggg
     1861 cagacgccac gcgtggccgg tgtccccccc ttggtaaaaa cgggttgggg atcgctcaat
     1921 tcgcgcttgg ttttacattt aacacaataa gcttccaaaa tgattacctc catataagtg
     1981 ctatctctta ccaattacac aggcctcaga tgtagctacg cccgctctct tttaacgact
     2041 ttacgatctg ccgcaccttc tgagcttgat cctttcggca aaccagcaac acatcaccgg
     2101 tgtccaccag taccagatcc tggacaccaa tggtcacaat taggcggtgt tcctgattta
     2161 catagacaag cgtttcgcga gtatccaaac cgatatgctc gccgcccatc acgatgttac
     2221 cttgtggatc ggaaggtagc acatcgaaga gtgagtccca gcttcccacg tcattccatc
     2281 gcaggtcatc agccatgatg actgctacat ctttggcgcc ctccatgatg ccataatcaa
     2341 tggtctctga taccagttga tcccaaattc ggtaaataac cgtttgctgc tgtgacgaac
     2401 cccacacctg ctggatctct tgcaacccag aatatagttt tggcatctgt gtggcaaatt
     2461 cagacagcac gcgatctact cgccacacga acatgccaga gttccaagca tgatcacctt
     2521 gtttcaacat gcgacgcgcc cgtgcttcat ccggtttttc acgaaagcga gctacgcgga
     2581 aagcgtcata gttgcgataa gtggtgatac gttccccgcg ctgaatataa ccgtatgctg
     2641 tagctggata gctgggcgtg atacccaatg ttaccagatg attatcttgc gccgcattat
     2701 gagcggctgc cagcatcttc cggaagcctt cctcgttgcc tatgaaatga tccgatgtca
     2761 ggatagccat caccgcctgt ggatcgcgct gttgcagcgc tacagccgcc aagccaacca
     2821 ctgatgcggt gcccctcggc ataggctcca ataaaaaatt ttccttcggg atggcaggag
     2881 attgtaattg cagttctgct gcctgctcag cgacagtcac cacgtaaatt cgctcaggcg
     2941 ggaataagcc ctccaggcgt tgcacggaag tctggaaaag gctgcgctct tcgattaagc
     3001 gtaagaactg tttcggcttt gcctgtcgcg aaagtggcca aaggcgggtg ccacctcccc
     3061 cagccatgat aattgcgtaa aagtgatcga acatagatac ctgattactt tatgatgtaa
     3121 tcctctgcct cttctcggat tgccacgaaa ttcatcccgc ctagctgccg taccatccct
     3181 ttgagctcca tcagcgctag tgtggctgag accttctcaa tgggcatgtc cacccgggct
     3241 cgtatatcgt ctacgtgcat gggttctctg gtgagcacgc tcagcagcct ggcttcgacc
     3301 tcatccttgg gcaagaccgc tcgagctaca cgttgctgct ggaaagaagc caggtcaaga
     3361 ctttctagca cgtcctcagg ggtcaatagg gggcgagcgc cctgatggat caggcgattg
     3421 cagcccacgc ttttgggtgc attgatgttt ccaggcacag cgaacacctc tcgcccctga
     3481 tcggcagcaa actcagccgt gatcaaagct ccgctacgct cgcctgcctc aataacaatg
     3541 actgccatag aaaggcccga gatgatgcgg ttgcggggtg gaaagttgac accatcgggc
     3601 ggtgtacctg gcgcataatc gctcatcaag gcgccactcg ctatgatctg atcagcaagc
     3661 tggcgatgct ccggaggata aatttggtca actccgcttc ccaacacagc caatgtccta
     3721 cctccagcat tcaatgcagc cttatgcgcc tcggcatcga cgccccgtgc cagaccactg
     3781 attaccgtca ccccgcggcg tgccaatacg ctggcaattt ccgcagtcac ctgacgacca
     3841 taagcagtga tccctcgcgt gccgacgatc gccacagccc actcatcttc tggttgaagg
     3901 ttgtcacgca ggtagagcac aggtggaggc tggtcgatct ccttaaggcg tctggggtag
     3961 gcttcatctt cccaggtcag cacctggatg ccaaggctgt gcacccgctc ccaaaccagc
     4021 tctaacgaaa cacccgcgcg tacttgttgc atagtctcga tcagcttggg acctagtcct
     4081 gcggcgcgca tttcttgagg agaagcgtgc caggctgtct ctacgtcccc aaaatgctgg
     4141 atcagattac gcaggcggac cgcaccaatg cctttgacaa ggttaaaacc cacccaatac
     4201 gcttttggat caatcaagag tactcctctt taaggaagaa gcccaccggt ttaaaaaacg
     4261 tggacagaat accacaatca aattatgtgt gtcaagagat ggtcatccac cttgagttta
     4321 cctcaatccc tccaacaaac gcacgcgcat ttccccttga tcaagatcaa tctttaggac
     4381 aatcgaatca atcacaggca tcagaaaatc cgacccttcc gaaggccgca cgatacagac
     4441 atcgttcgca cccgtctcca ggatatcaac cacggtaccc aggaactctc catcctcgct
     4501 gtacacacgc agcccaagga tttgatggtg gtaataatcc ccttcgggta aggcgggtat
     4561 atcatcagca cgcacatata gtaacttatt tcgcaaggca cccaccgcat cgcggtccaa
     4621 tatttcttta aatgccaata gcagattctt atcatgagga cggcatttgc gaatttgcaa
     4681 tgggaggtgg ttctcgccca cataaaccgt cattccagag cgcatacgtt cgggaaaatc
     4741 ggtgatgaca tccatcaaaa tttcgccgct cacgccatgc ggccgacgca acctgcctac
     4801 cactaagaag acaggctcgc ccgacggagg caagcctgtt gtctgctcag atgacggttc
     4861 ggaaaaagtc atcgcggctc atctacatcg agggaagcgc gcttcccttc ttgagcagcc
     4921 gccacacgca gcaagatacg catggcgttt gccacccgtc cgcctttgcc gatatcgcgc
     4981 cccatatctt ccttggcgac cttgagacgc aggctcattg agggaccccg ctggaattga
     5041 gaaacctgca cctgggtagg atcatccacc agtgatgttg caatatatga gattaattcc
     5101 ttcaccatcc gcctcctatc aagttatcag cagagaaact acaccctgct tggtcagctt
     5161 tcctgttata tacaaaaagc gtctttggat caggcttact tagctttact ggcgcgtctt
     5221 ggggctgatg gggtttgcct cagcttctgc ggcagcttcg gccaacaggg tctccaattc
     5281 ttcaccctgc ttataacgtg cgtaacggtc aagcaaaccc gtggtgcgga acagtctagc
     5341 cactgaatcg ctaggctgag ctccctggct catccagtgg tatacgcgat cctccttcac
     5401 cagaatagtg gctggaactg tgcgcggatt ataggaacct aggatttcca ggaagcgccc
     5461 atcgcgcgga ctttctttgt ctgcagccac aatgcgatac gttggctggc ctttggctcc
     5521 catgcggcgc aatcgaattt ttaccattga actttctcct tcaataaata tgttctaaat
     5581 atatctcaaa atggtgtggg tattttacag cgaaaagtcc aatgcaaaat acctgctcat
     5641 tttgttctta ccttctaacc aaacaaacgt gggatgccgc gtttgcccga tttttttaat
     5701 gatttaaaga ggcgctgtgc atcgcggtat tgcttgatca gacgatttac atcctgaaca
     5761 tgcgtaccgg atccactggc gattcgacgc cgacggctag cgttgaggat atccggcttc
     5821 cgccgttcat ccttggtcat cgaaccaatg atggcttccg tccttttaaa ttgtctctcg
     5881 gcatcttgtg gattgatctg ccgagccatt tggcccatcc cgccgggaag catgtccatc
     5941 aactgtccca atgggcccat tttgcgcaat tggctgagtt ggtcggcaaa gtcctgcaga
     6001 gtgaactcac cagagagcat cctgtttgcc tgctccgcgg ctaccttctc atcaaaggct
     6061 gcctcagcct tttcgatcaa tccgatcatg tcgcccatgc cgagaatacg ggaagacagg
     6121 cgagccggat cgtatgcctc aatggcatcc aaggcttcac ctgttcccag gaacttgatc
     6181 ggcacaccag tgaccgagcg aatagatatt gccgcaccgc cacgagcatc gccgtccatc
     6241 ttggtcaaga gcaggcctgt caatgttacc acatcgcgaa atcctttggc gatattgact
     6301 gcctcctggc caatcatggc atccaccacc agcagggtct cgaccgggtc gacgcgacgc
     6361 ctaatctctt gaagctcgtt catcaacgca tcatccaact gagagcgccc ggctgtatct
     6421 aagatcagga cggtaaagcc tcccttttgg gctttgtcgc gtgcgcggac cgccagttga
     6481 ggaggctgga tgccctcttc gaagtgcaca gggacgttca ggcgctcgcc taaagtctct
     6541 agctggcgca ccgccgccgg ccgataggga tcgcaggcta ccaacatcac cctctcaccc
     6601 tgggcgcgta gtaggcgggc tagcttacca gctgccgtgg tctttccaga gccctgcaga
     6661 ccgaccagca taattgtgcg cggcttgggg ccactcaggt caaagcggtc tggagcaccc
     6721 agcgtggcaa tcaactcttc gttaacgatc tttaccacct gctgccctgg gttgagcgct
     6781 ttagatacct cataaccgat ggcgcgctgg cgtacccgag ctacaaaatc ctttacgacg
     6841 gcataattga catcagcttc aagcagcgcc aagcgcactt cacgcatggc ggcatccaca
     6901 tcgctctccg agagcttccc tctccggcga agttgcttga agacattttc gagacgttcg
     6961 gtcaggttct caaacacagc attgcatttt agtatggaag caaggaatgg tcaagtagac
     7021 atttagaaaa accgcggggc aacgagaatg gaagttggat ctcttaaaca agtgaacgcc
     7081 cgatgaaaat aattcaaaat attggttata attacattca aaggattgaa tattgtgctt
     7141 caagataaat cttcacccgt gatcgatgat ccagccaagc tattcaaaat tctcatgcac
     7201 ccggctcggt tgtctatatt ggatatttta cgcgatagtg aacactgtgt ctgccacatg
     7261 gaatcacacc tggggtaccg gcaggcatat atctcgcagc aattgatggt cttacgcgaa
     7321 gccggcctgg tggaagatcg caaagatggg ctgaacgttt tttatcgtgt gaccgtccca
     7381 accctttacg aggtaatcga tgtggcgcgt aaaatcactt acgtcgacga gcaaatacag
     7441 gaacacgatc cccaccacga agcaaaagca gcttcctgcc cttgccccaa gtgcaatcca
     7501 gccgctaaaa atgtatcttg ctaagtccgt acagcagatg tcatagcaaa cctgtgattt
     7561 tattcactat gtctaattaa ttgaaagaga tatactatat tcaatgattt gaatataact
     7621 tcatgcatag gacaatgcca tgctggagat caagatttta ggcactggct gcgccggatg
     7681 cttaaaaaca gaacaagtag ttcacgaggc cttacgagcg ctgggcattc aaaacgctaa
     7741 cgtagagctt gtcgccgagc agcgcatgat tgagtacggt ttgttagcag atcgtgcgcc
     7801 aggtcttttg atcaatggat atttggcttg ggcgggtaca gtgccaacaa ttgaacaggt
     7861 catggagtgg gtcacgcaag cagtaacccc aactgtgatc tagactacac gattattttg
     7921 cgcatccttt acccaagccc aacctggaca tccaagagta tgacatgaca gatttcctcc
     7981 aaatcaccgt cgataaattc acttttaaag tcgctaccga ccgttactat aatgaagagg
     8041 gggtttgggt gctagcccaa gggaaccgcg tgcgcattgg attgtccgat ttcctgcaac
     8101 agcgtagcgg agatgtggcc ttcgctgaaa ttacgccgca aggaactgtg ctggcagtgg
     8161 gcgatgaaat agccgtgatt gagacagtca aagtaaatat cagcctctca tcaccggtat
     8221 caggatcaat cgtcgagatc aatccgctca tggaaacagc cccggaaatt atcaaccaag
     8281 atccatatgg tgaaggttgg ctagcaatta ttgaggccac ccagtgggag agcgactctt
     8341 cccgcctgct cgatccacaa gcgtactact cccggatgca aatccaggct gaggagtcag
     8401 tataggtatg agccaagtta gtgtgatcat cgtcccttgc agcggtatcg gcaagaccta
     8461 tggaacggtc agccgcgaag ctgcttatga aattaccgaa gagctgcgac ctgaccaaac
     8521 tcaattggta ccattgtctc tgctggtgct gggtgacgag gctacacaca gcacggtgat
     8581 gggaaatcca gccattacca tagatggctg taagctggct tgcgcgacca aaatggtcaa
     8641 agaatgtggc gggcaggtgg ctcacaatat cgctgtcttg gacgtttacc ggcgccacaa
     8701 gcagttcaag cctcagggga ttgccatact caatgagggt ggcttgaaac tcgctcacgc
     8761 actggctgaa gagatactgc ccatggcaga ccagctaatg gaaattgccc gtaagagaga
     8821 gccatatggt tgatttgccc aaaaagaaag tcgggatcat cgcctgttcc ggagaagaac
     8881 taccggaagg cacagtgacg cgattggcag cattaagagt gctggaggag ttgcgtccca
     8941 gtgacacggt aacgatttgc ttgcctttat ttttagcggg tggtgaagga gaccgggcat
     9001 ttgcccgctt ttaccccact attgccttag acggctgcga aaaacgctgc gccgctcgag
     9061 gcacggagat gtactcgggc aaaccagctg ccagtgtggt ggtcagcgag ctcatccatc
     9121 aaaactctct tccccaaccg cgcgggcggc gcaagttaga tgaggcggga agatacattg
     9181 taaatttttc agccgagcac gtggcaaatt tggtagatga tctgttagat aaacggtgga
     9241 accgacaggc aggcaagttt gcagaagaaa cacatcctgc cgcctcgagt ttgcaaacaa
     9301 cttgcacatg cgggtcggat atcccttccc aagcaatctt catcgaaggg caagcggtta
     9361 ccataattgc attaccgcta atctttcaac aactgcacga agctggcaaa ataatccgcc
     9421 aggataatgc cagggagctg ctcgagctgg tcagggttta caaccccatc cctaaccgag
     9481 ccgaagaggc gtacacaagc gcacttttgc gtgaatacca tacctactgc aagcagaaag
     9541 ccacttcaca atgagccgga cagagacgta ccacaccacg gtaacctgga agaatgggca
     9601 ttggggtcac attgccatgg gtaacggagc agaaatggat ttttctgcgc cagctgaggc
     9661 gcatggtcat ccaggtgtcc tgaccccaga ggacgctttc gtggctgcag ccaacacctg
     9721 catcatgatg atgtttcttt ggaccgcgga gcgctttaag ctaaagctac tctcttacga
     9781 atgcagagcg gagggcacaa aactaatcga attagaccgt acagagatct tcactcacct
     9841 gcacttgtgg ccagttataa gcatctcagc agcaggtgaa gatcgggggg tgataaatgc
     9901 acgtgcgcaa cgggccatgc aatctgctca aaaatatagc ttggtcgcaa attcaataaa
     9961 atccgagatc gtgattcagc caaaaatcga agtcattgaa tgaaacaaag acaacagcca
    10021 tagtgaacaa ctaaaacaat tttcat
//
LOCUS       DBNGSX010000236         3323 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1611679, whole genome shotgun sequence.
ACCESSION   DBNGSX010000236 DBNGSX010000000
VERSION     DBNGSX010000236.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1611679"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            506..1795
                     /locus_tag="AC16CB_07215"
     CDS             506..1795
                     /locus_tag="AC16CB_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342295.1"
                     /translation="MGITLLYIYHGARILFAAIMSLAIFRFCQTYLSIGKSSRLAFLL
                     AIFGSGLGWLVLPFDAFTSDFWVAEAYPFLSAYTNPHFALSLTFLLFLLSPVKVPAAN
                     QSMRKSLVYSVLRISLPSFALAMMSPFAVVIALSIWAGMLFWDFLQATLEHSKVELRG
                     YIYRLVWIGIGGAPILVYQYIVMNSDPLFSEWMIQNITPSPPIWDAMISLSPLLLLVP
                     YGAWVIILNRNKEQRLLLVWVVVSLILLYTPFSLQRRFMMGIYVPVAILAAMSIDWMI
                     KDGRRFWFRSMLLLLIVLPTNLLILRAAWHGIQTRDDRLYLSKDEIDALAWVEENSPS
                     DALILAAPETGLFIPAITGRRVIYGHPFETVHAEKQEERVLNFYQGVALPNPKAWLGL
                     VDYVYYGPREREIGKNELLDALKAVFQNQSVVIYATK"
     gene            1792..>3323
                     /locus_tag="AC16CB_07220"
     CDS             1792..>3323
                     /locus_tag="AC16CB_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342296.1"
                     /translation="MNGGVLGKHWKKPLRDSRVVGFLLLLPFLVILPNLDTFPYPRYT
                     GAYSDITVSHYPNAYFLRQELLAHRPIPLWSPTILSGYPFIANPLSGLLYPPGWLGLL
                     LPIPAGFHVVIALHLVLGGVGMLRLLRAEGMSYPAALFGGMAFELMPKIAAHYGAGHL
                     TLLYALAWTPWLLLTWKEEEKQRVDKGNESKRWIKPSLVLTMIILADVRWAAFATLLL
                     WAYAIAHSHYFLVAIRDLTRQTGLAVLLSAPLSIPLIEFIILSSRTTMTAADRLTLSL
                     SPARILGFIFPDFGGFHEWALYPGAVTLCLALVAIAGIRHKSGLAFWIWVAVASTLVA
                     LGSYIPGMQYLARLPGFSLLRVPARALFITGLAIAALAGYGLEALIGQFRGLNRRVVK
                     LVLAGLVSFALVLAAGVRFTTLNISLSFLWGAGITLLSVLWIMWCINGVAKRFENFWF
                     VGLFVIAIIDWTAVDRSYVLFRPFGEVQTENAASAEWLASQPGLFRVYSPSYSIPQAT
                     AI"
BASE COUNT          742 a          717 c          823 g         1041 t
ORIGIN      
        1 tttctcccct gatctagctg gctatcgagc tgctgcaaac gaaataactt aaagggttgg
       61 ctcatgatct gaaatgatta tagagggggt gaaggataat ggcaagaggc aaagcataac
      121 ctgacaaaaa tgaaaaacta ttatcatggt ctgaataaat ttgagacttc accgccatgc
      181 tataatcgaa aataatttac attcccaggg taagcctctg atctcactca ttccctcctc
      241 ccgcgatcga ctcttcgtgc tgtgtgttac ggtaatatta ttggtattta tcacagttcc
      301 atatttcgtt gcggaactaa gcgcaggcga gtcgctggtt ttcggtggat tcctgctcaa
      361 ccctattgat gggatgagct atttagccaa aatataccag ggttggcgtg gggattggct
      421 tttcaccttg ccttatactg cagagaaggg gcagggtgct tttatctacc cgttttacat
      481 ttttcttggt cacctggcac gctccttggg aattaccctg ctgtatatct atcatggggc
      541 tagaatactt tttgcagcga tcatgtcgct ggcaatattc cgattttgcc aaacatatct
      601 ttccattggg aaaagctcgc gcctagcttt tctgctggct atttttggct ccggtttggg
      661 atggcttgtg ctaccatttg acgcttttac gtcagatttt tgggtggctg aggcttatcc
      721 ttttctctct gcatacacaa atccgcattt cgctttgagc ctgacgtttc tgctgttctt
      781 acttagtcca gtcaaagttc ctgctgcaaa ccagagtatg agaaaatctc ttgtctatag
      841 cgttttgaga atttccctac cctcgtttgc gttagcgatg atgtccccat ttgcagttgt
      901 gattgcatta agtatatggg cgggtatgct gttttgggat ttccttcagg ctactctgga
      961 gcattccaag gttgagttgc gtggatatat ttaccgccta gtatggatcg gtataggtgg
     1021 cgcgcccatt ctggtttacc aatatatcgt catgaattcg gatcccttgt tttctgaatg
     1081 gatgatccaa aatattacgc catccccacc aatttgggat gccatgatct cactttcgcc
     1141 gttgttgtta ctggttccat atggggcttg ggtgataatt cttaacagga acaaggaaca
     1201 gcgattattg cttgtgtggg tcgttgtaag cttaatcttg ctgtacacac cttttagttt
     1261 gcaacgacgc ttcatgatgg gcatctacgt ccctgtagcc atcctagcag ctatgagcat
     1321 tgattggatg atcaaggatg ggcgtcgatt ttggtttcgc agtatgctac tgttgctcat
     1381 agtattgcca acgaatttac tcatcttgcg ggcagcctgg catggaattc aaacccgcga
     1441 cgatcgtcta tatcttagta aggatgaaat agatgcgttg gcatgggttg aagaaaattc
     1501 accctctgat gccctcattc tggcggctcc agaaacgggt ttatttatac ctgcaataac
     1561 tggccgacgt gttatttacg gtcatccatt tgagacagtt cacgctgaaa agcaggaaga
     1621 acgagtgtta aatttctacc agggtgtagc tttgccaaat ccgaaggctt ggttaggctt
     1681 ggtggattat gtatattatg gaccacgtga aagagaaata ggcaaaaatg aattactcga
     1741 tgcgttgaaa gcagtattcc aaaatcagag tgtggtcatt tatgcgacaa aatgaatggt
     1801 ggtgtgttgg gtaagcactg gaaaaaacct ttgcgggata gccgggtggt cggcttcttg
     1861 ttgctgctgc catttcttgt catccttcca aacttagata cttttccata tccacgttac
     1921 actggggctt attcggatat tacggtcagc cattatccca acgcgtactt cttgcggcag
     1981 gaattattgg ctcaccgccc gatcccctta tggtcaccga cgatcctgag tggctaccca
     2041 tttattgcta atccactatc gggtctgtta tatccaccag gttggcttgg gctgctattg
     2101 ccaataccgg ctggattcca tgttgtgatc gctctgcatc ttgtgttggg tggtgttggg
     2161 atgctgcgct tgttgcgcgc agaaggcatg agttatcctg cagctctttt tggggggatg
     2221 gcattcgagt taatgccaaa gatcgcagcc cattatggag cgggccactt gacactcctg
     2281 tatgcacttg cctggacacc gtggctatta ttaacctgga aagaggagga aaagcaaaga
     2341 gttgataagg ggaatgagtc caagcgctgg ataaaaccga gtctggttct aacgatgata
     2401 attctggcag atgtacgctg ggctgcattt gctaccctgt tgttatgggc atacgctatt
     2461 gcgcatagcc attatttcct ggttgctatt cgagatttga ctcgacaaac tggattggct
     2521 gtcttgcttt ctgctccatt gtcgatacct ctaattgaat ttattatcct ctcgtccagg
     2581 accactatga cggcggcaga tcgcttaaca ttgtcattgt cacccgcacg catattaggt
     2641 tttatatttc cggattttgg cggttttcat gagtgggcgc tttatcctgg agctgtgact
     2701 ctttgtcttg cactggtggc aattgcaggt atcagacata aatctggtct agcattttgg
     2761 atttgggtag cggtggcaag tacactggtg gcgttaggta gctatatacc agggatgcaa
     2821 tacctggctc gtctacccgg ctttagcctg ttacgcgttc cagcgcgtgc cttattcata
     2881 acgggacttg ctatagctgc attggcagga tatggactcg aggcactaat tggccaattc
     2941 agagggctga ataggcgagt tgtaaagctc gtcctggctg ggctggtgag ctttgcgctg
     3001 gttttggcgg ccggagttag attcaccacc cttaatatct cgctttcgtt tttatggggt
     3061 gcggggataa ctttgctttc tgtactctgg attatgtggt gtatcaatgg ggtagcaaaa
     3121 cgttttgaaa acttctggtt tgtggggttg tttgtcatcg ccataatcga ttggacagct
     3181 gttgatcgtt cttacgtatt atttcgccca tttggagagg tccagactga gaatgcagct
     3241 tccgccgaat ggttagctag ccaacctggg ttgtttcgag tgtactcacc ctcctatagt
     3301 attccccaag caacagccat tca
//
LOCUS       DBNGSX010000237        12187 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1889079, whole genome shotgun sequence.
ACCESSION   DBNGSX010000237 DBNGSX010000000
VERSION     DBNGSX010000237.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12187)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12187)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12187
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1889079"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="AC16CB_07225"
     CDS             <1..297
                     /locus_tag="AC16CB_07225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsmA family protein"
                     /protein_id="HIR3342297.1"
                     /translation="RWQGRLKGWHLVFFWAGLVCDASGTGIMFDMADADLFSLHGLTG
                     LLAIVLMFVHAVWASLVLYRKDERAITNFHRFSVVVWLIWLVPYFNGFFMGMGE"
     gene            complement(319..1338)
                     /locus_tag="AC16CB_07230"
     CDS             complement(319..1338)
                     /locus_tag="AC16CB_07230"
                     /inference="COORDINATES: protein motif:HMM:NF014420.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIR3342298.1"
                     /translation="MAIEYQKGNSLLHKLDARTKLLLFAGATVLAVIIVDPIIMAALF
                     FLLYLMGTKAVDRKNLNRNLRVLVVIFFTFSLFQIFFFTPSDAHFLFFLIPFKNWVPV
                     TVEGIIRGVAVFFRFFSVVLAVHLMLYTTPPVDMVLAITKRERNRSLLQHIGLMALFG
                     LFFLVIAFLVMPQTIQRIPLPLILRGLLVFAISFVVAVVIEKILSNGLPPEMGVALTL
                     GFATVGILSQQTQKITDAQKARGYDVQPKNLIRRVQVLTALLLPIFLATLERSQDIAI
                     AILARGFDYNIAARTYRRQLSFTKIDYIFQASIILLLIGGLVLNYYGVGNPTEQLVMK
                     ILGYQ"
     gene            complement(1328..3037)
                     /locus_tag="AC16CB_07235"
     CDS             complement(1328..3037)
                     /locus_tag="AC16CB_07235"
                     /inference="COORDINATES: protein motif:HMM:NF010167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3342299.1"
                     /translation="MDYDITFRNVSFTYEGADIPALQDITLTIMPGEIVLITGPAGSG
                     KTTLCSCMNGLIPHYHEGELTGEVLVRTYNTRKARIGGLASLVGMVFQDPESQLVTNS
                     VADEVAFGPENLGVAREDINLRVQEALQATRLKGYEDREPHSLSGGEQQACVIASIYA
                     MHPEIYVMDEPLANLDPVGRVQVLRVVVEIAKQRGKTLVLVEHSLEEVLPLVDRVIVL
                     DQGRIIRDSSTADVLAAGDIPHVFTRPPIIRLAEKFSLTPLPLTSDQFYQSFSEKYPL
                     RSTNWTVPEKPVLKAGTPVIEMRDVNYSYDNKNIVLNDVNLTINAGELVAILGRNGSG
                     KTTLVRHIIGLLQPIRGKVIVMGKDVSVTPTHEIAQDVGFCFQNPNHQIVSFNVRDEL
                     LFGLKAHHISPTEFETRIHEALEFVDMLDLVNAEIFDLGKGQKQRLALASVLTLKPRI
                     LIIDEPTTGQDPRMSEEIFEIIKRLNEIGTTVLMITHKIDYAAIYAKRAIVLKHGRIA
                     FDGPIQELMADLETMQANSLDLPETTKLAIRLSKHGTPPWLVSMEDVEESLRHLLEAS
                     NGN"
     gene            complement(3212..3781)
                     /locus_tag="AC16CB_07240"
     CDS             complement(3212..3781)
                     /locus_tag="AC16CB_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342300.1"
                     /translation="MSDQVSIESERKPLLSARQVGVAAAFGGAALALVLAGVTIPIPG
                     TPVVTDPREIFTTTGASLTGPIGGIVIGILAGIAEPGIPLASLLAHIIGGIYSGFLYK
                     NLAWRFRENRVSYLALWALQVLGYYFIVVVPLFVVGLVLFYPDPEYGGFFALLAALEG
                     GAAPEAIITTVVTTIIMAALPERYRRPLW"
     gene            3867..4625
                     /locus_tag="AC16CB_07245"
     CDS             3867..4625
                     /locus_tag="AC16CB_07245"
                     /EC_number="3.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013513632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016403 - dimethylargininase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylarginine dimethylaminohydrolase family
                     protein"
                     /protein_id="HIR3342301.1"
                     /translation="MFTHAIVRKPGMNFAQGITSANLGTPDYAHMVDQHQAYIQALES
                     LGLEVIVLDAELNYPDAYFVEDVAIVTPDVAVITNPGAPARKGEEDSIEPVLARYRPT
                     VRIQPPGTVEGGDVLMVGTHFFIGISERTNLEGATQLGNVLERYGNTFTLVHIQAGLH
                     LKSSVNYAGKNTLLVCEDFAERGEFQGYRTIVVPQIEAYAANTLWANDHLITPAGFPE
                     TRKRLVALDLPIIELDVSEAHKMDGGLTCMSLRF"
     gene            4657..5127
                     /locus_tag="AC16CB_07250"
     CDS             4657..5127
                     /locus_tag="AC16CB_07250"
                     /inference="COORDINATES: protein motif:HMM:NF024052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HIR3342302.1"
                     /translation="MTEHLTPEKLHVTYIDDVRNDGPLAPRAYTLTHSDTTGDLFLTI
                     GQRYNRSQISGLYTRFMRDEVLAEWRQEEQLTLHVHCHVSGGLVFGSSGYRDSIFRYH
                     LPMVLEAFRYGDQKLVEAHPQLAQAPIMVHFHAREDKFDKTEQWGVFDEYQISD"
     gene            5206..6234
                     /locus_tag="AC16CB_07255"
     CDS             5206..6234
                     /locus_tag="AC16CB_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR3342303.1"
                     /translation="MKAVRMVDVNLPLQMFDIPIPALGEGDILVRIRAAGICHSDAHY
                     RAGKSPVRPLPMTLGHEIAGIVEEVGKRVTSVKVGDRVCLHYLLSCGECSYCRIGSEQ
                     FCTQGSMLGHYSDGGYAEYIAVPERNAVHLPDEISFEQGAIMMCSSATSFHALRKSRL
                     RAGETVAIFGIGGLGISAVQLASLFGALEVFAVDINPEKLKLVERYGAIPINATLNDP
                     VAEIRRLTHNKGVDVALELIGLSQTMKQAVRSLAFMGRAVIAGISDKPLEIDTYWELL
                     GKETEVIGTNDHLLSELPTIIEFARQGKLDLSGVITRTVPLEADHINRALDNLEKFGD
                     DIRTVIIS"
     gene            6300..10016
                     /locus_tag="AC16CB_07260"
     CDS             6300..10016
                     /locus_tag="AC16CB_07260"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3342304.1"
                     /translation="MNEQEQLEHAIAALEAQRSLLGDSVVDAALASMRQRLAILRSPP
                     LAEQRKTVTILFADLVDFTAMSEQLDPEDVREIVNAYFTRCTEAIEMYGGNIEKFIGD
                     AVMAVFGLVEAREDDPENAVRAALNIRQALSELNQEVQARWEMRLRMRIGIHTGPVVV
                     SLLSERKGMDFVVVGDAVNLASRLQSVAPADGILISHSTYRHVRGNFNVQVIEPIRIK
                     GKSESIQAYVILEAKSRPFRLETRGVEGIETRMVGREAEFQLLSDALDQMVRQGQNRI
                     VTLVGEAGVGKSRLIYEFINWLELLPENFTFFKGRAGPAMQNAVFSLLRDLFSFRFQI
                     QDSDPPQVVQEKMERGIYWAYETLPKLEQGGDHPAKQLSIQEIQKRAHFIGHLVGFDF
                     LDSPYLRVDKHDARYLRDRAMVYLADYYRTLAVYRPIVIVLEDLQWADDNSLDVFSRM
                     TSILPQSDSSHGVLVLCAARPDFFTRLPGWDEIQPLNVRIDLQSLDRVTCIELVDEIL
                     QKIPRVPAELRELVIASTDGNPLYIEEFIKMLIEDQVIVKADEEWQIDLAQLTSIHIP
                     PTLTGILQARLDSLPVGERLVLHRASVIGRVFWDAAVEYLAENERSEDMPISAPTAGL
                     LDRLRSREMVDQRKESVFEKTREYLFRHALFRDVAYESVLKRQRSLYHAHAARWLEAV
                     TQRSGRADEFSLLIADHYDRANEAQPAAKWYYQAGLQAAEHFANREAIRYFNRCLELL
                     ADGSPEDRFPAILAREKVFELLGERQLQHEDLESLTRLVDEMTPGKETDTRRVQVLLR
                     RSRLSEATSDNVASIEYARRALKHAQIASLDESMASCYLLWGSALWRQGKYVEARQPL
                     EKALEIARRVSIPNLEADILRNLGIVNAVLSNYTEARLYYEQSLAIKRELGDLRGESA
                     TLNSLSMLVYDLGDYLQARQYLEQSLNIKREIGDRRGEEIAMNNLGLVAYSEGDPVAA
                     RRYMGEALSMSQEILDLEGQTTALAVLGLIATYQGDYAAAQDYLENSLNLFREQVNDV
                     QGESEALSYMALLQNRLGNQEVAQEYATRALELARDVGAWREQFNALLFLGHALLALN
                     RTEEAIQAYQQALDLPDKERQTSPHLEAVAGLGRAWLEKWQVNSGISAGMANDALEQA
                     RNCVEIILDHLATKPLQDVHEPARIYLTCYNVLHASRDARASWVLEQAYHWLNDRAST
                     FQDEALKRLFLDNIPANRIISAEWETAHQLEDKP"
     gene            10013..11278
                     /locus_tag="AC16CB_07265"
     CDS             10013..11278
                     /locus_tag="AC16CB_07265"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR3342305.1"
                     /translation="MNRDLIRQVPLFSQLPDAELDYLAQTLQYCERGPDTILFREGDL
                     SQCFMAILDGQIEIVKALGTPEETVLTVEGPGEILGEISLFLPDRNRSASARTLTHVQ
                     LFELSPAGFHTLLHRNPSLALPIMQVLTQRLRNSENTTIRDLQQKNIQLSDALQELRA
                     AQEQLIEKEKLEHELQVARQIQERFLPKELPSLPGWEIAAFWQPARAVGGDFYDFVVF
                     PNGQLGIVVGDVSGKGVPAALIMATTRSVMRAVTEHHFSPGSILERVNDLLNPDMPSN
                     MFVTCVIAVLEPSSGHLRLANAGHDLPHHLALDGSLELHATGMPLGLLPGMRYDEIET
                     TLSDGDSLLFYSDGLVEARDTQGEMYGFPRVRKFLSPWAQQKMASLTPDGASELLIRD
                     LLESMQAFTGPTIEQDDDVTFVTLCRLQI"
     gene            complement(11364..>12187)
                     /locus_tag="AC16CB_07270"
     CDS             complement(11364..>12187)
                     /locus_tag="AC16CB_07270"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR3342306.1"
                     /translation="GSVTILRAEMLTKSTREAPWRLDVDCNGTLRSFVLRLDSRGIEH
                     EYRVLRAMQSIPIPTPRAYGWDAEGEALGLPCFFSDFIEGEPLLAPMLAGNTWAEELY
                     LDTVIALQEITQDDLAEIAHLLDRQTAADVLEEAQGYFLRNPQPLAAQAYAALKDKMP
                     ELPAIRFSNGDLWLENFLVRDQQLAGVIDFANAGFSDPIFEFLLSFFVSPELRGRGIE
                     ARYCRRLGYDPEILQWYHGLEYFDTWRWVLYSGEPFVHHTAESLERDLAKWLEEF"
BASE COUNT         2918 a         3070 c         3273 g         2926 t
ORIGIN      
        1 cgctggcaag gtcgattgaa gggctggcac ctggttttct tttgggcggg tctggtttgt
       61 gatgccagcg gaactggtat catgtttgat atggcagatg ccgatttgtt cagcctccat
      121 gggctaacag ggctgctggc aattgtgctg atgttcgtgc acgctgtttg ggctagtctt
      181 gtgctctacc gcaaagatga acgcgccatc accaatttcc accgcttcag cgtcgtggtc
      241 tggttgattt ggctggtgcc ctactttaac ggattcttta tgggtatggg ggagtgagtt
      301 gctagaacga tagcctggtt attggtaacc cagaattttc atcaccagct gttcggttgg
      361 gtttcccaca ccataatagt ttaagaccaa tcctccaatg agtaacaaga taatggaggc
      421 ttgaaagatg tagtcgattt tggtgaagga cagctgccgg cgataggtgc gcgcggcgat
      481 attgtagtca aagccacgag ccagaattgc aatcgcaata tcctgcgatc gctcgagagt
      541 agccagaaat atggggagca gcagggcggt gagcacctga actcggcgaa tgagattctt
      601 cggttgcaca tcatagccac gcgctttctg agcatcggtg attttttgcg tttgctgaga
      661 gagaataccc accgtagcga agcccagggt caatgcaacc cccatttctg gcggaagccc
      721 gttgcttaat attttttcga taacaactgc gaccacaaac gagatggcaa agacgagcaa
      781 gccgcgtaaa attagtggta atgggattcg ttggattgtt tgcggcataa ccaggaacgc
      841 gatgaccaaa aagaacaatc cgaagagagc catgagccca atgtgttgaa gtaaactgcg
      901 attgcgttct cgctttgtga tggctaagac catgtcaact ggcggggtgg tatacaacat
      961 caggtgaacc gccaaaacca cactgaaaaa gcggaagaat acggctacac cccggatgat
     1021 gccctccaca gtcactggca cccaattctt aaagggaata aggaaaaaga ggaaatgagc
     1081 atcgctcggc gtgaagaaaa atatctgaaa caaagagaat gtaaaaaaga tgactaccag
     1141 cacgcgcagg ttgcgattca ggtttttccg gtctacagcc tttgtgccca tcaggtaaag
     1201 caaaaaaaac aaggcagcca tgataatggg atcaacgatg atcactgcca gcaccgttgc
     1261 tcctgcgaac aacaagagct tggtcctggc atccagcttg tgcagcaatg agtttccctt
     1321 ctggtactca attgccattg ctcgcctcca gcaaatgtct gagcgattct tcgacatcct
     1381 ccatggaaac caaccagggg ggagtgccat gtttgcttaa gcgaatcgcc agctttgtcg
     1441 tctcgggtag atcaagcgag tttgcctgca tggtttcaag atcggccatt aattcttgaa
     1501 tagggccatc aaaagcgatc ctgccatgct tcaatacgat ggcacgtttt gcatatatgg
     1561 cagcgtaatc gatcttgtgg gtgatcatca ataccgtcgt accgatttcg ttgaggcgct
     1621 tgatgatctc gaagatctcc tcgctcattc ttgggtcctg accggtggtt ggctcgtcaa
     1681 taataaggat tcggggtttc agagtgagta cagatgccag agccagcctt tgtttttgcc
     1741 ctttccccag gtcaaatatc tcggcgttga ccaggtctag catatcgaca aattccaagg
     1801 cttcgtggat gcgcgtctca aattcggtgg gggagatatg atgcgcttta aggccaaata
     1861 gcaattcatc ccgcacattg aaagagacga tctggtgatt ggggttttgg aagcagaagc
     1921 ctacatcctg ggcaatctcg tgggtgggtg ttacagatac gtctttaccc atcacgatca
     1981 ctttgccacg gatgggctgc aacaagccaa tgatgtggcg caccagtgtg gttttacccg
     2041 acccatttct ccctaagatt gcaaccagct cccccgcgtt gatggtgaga ttgacatcat
     2101 ttaagacgat atttttatta tcgtaggaat aatttacgtc tcgcatttca atgactggtg
     2161 tcccagcctt taaaactggc ttttctggta cggtccagtt ggttgatctc aatggatatt
     2221 tttcgctgaa ggattgataa aactggtcgg aggtgagtgg caagggggtt aagctaaatt
     2281 tttctgcgag ccggataatt ggaggccggg taaagacatg gggtatatcg cccgcagcca
     2341 gtacatcagc tgttgagcta tcgcggatta tccgcccttg gtcaagtacg atcacacgat
     2401 cgaccaaagg cagaacctct tccagcgaat gttcgaccag caccaatgtc tttcctcgct
     2461 gtttggctat ttcaacgacc acacgcagaa cctgaaccct gccaacgggg tctaggtttg
     2521 cgagaggctc atccatgacg taaatttctg ggtgcatggc gtagatggag gcgattacgc
     2581 acgcctgctg ttcccccccc gaaagactat gtggctcacg atcttcataa cccttgagcc
     2641 tagttgcctg aagagcctct tgaacacgca gattgatgtc ttctctggcg acgcccaggt
     2701 tttccggtcc aaaggcgacc tcgtcggcga cactattggt gacaagctgg ctttctggat
     2761 cctgaaaaac cattccgacc agtgaggcta atccgccgat gcgggctttg cgggtgttgt
     2821 aggtgcgcac aagcacctca cctgttaatt caccctcgtg atagtgcggg attaacccat
     2881 tcatgcagct acacagtgtc gtcttgccgc tgccggcagg gccagtgatt aaaacgattt
     2941 cacctggcat gatcgtgagg gtaatatcct gcagtgctgg aatatcagct ccctcgtatg
     3001 tgaatgacac attgcggaac gttatgtcgt aatccataaa cctgttttct tgaagattca
     3061 gtcgttacgg ttggcgagga aatggggggc tgccccccat ttcctccaac cgcgtcagtc
     3121 ctaaaaagtt aatagcccaa gtcgcaacct gtttccagga tgcggcttag gggttatatt
     3181 atcacccaaa attgttgggc tttgattaag attaccagag aggacgtcga taacgctctg
     3241 gcaaagccgc cataatgatg gtagttacga ctgtcgttat gatagcttcc ggagcggctc
     3301 cgccttccag cgcggcgagc aaggcaaaaa atccaccgta ttcaggatca gggtagaata
     3361 agactaatcc tactacaaac aagggtacga cgacgatgaa gtagtagcct aatacctgaa
     3421 gagcccataa tgccaggtaa gatacccgat tttcacggaa acgccaggcg aggttcttat
     3481 acaagaagcc actgtaaatc cccccaatga tatgtgccag caagctggcg agagggatac
     3541 cgggttcagc gatgcccgcc agaataccaa tcacaatacc accgatcgga cctgttaacg
     3601 aggcgcctgt ggtggtaaat atctcacgcg ggtcggtaac taccggtgtg cccgggatgg
     3661 ggatggttac accggcaagg acaagagcca aagctgcacc accaaatgca gctgcaacgc
     3721 ccacttggcg ggcggaaagc aaaggttttc tttcgctttc aatacttacc tgatcagaca
     3781 ttgaaatctc ctctctgaaa acgggttatt ttgaacttaa accctctagc atgttgaaag
     3841 ttacgataac accaggaggt tcccaaatgt tcacccacgc catcgttcgt aaacccggga
     3901 tgaatttcgc ccagggcatt accagcgcga atttaggaac tcctgattat gcccacatgg
     3961 tcgatcaaca ccaggcatat attcaagcgc ttgagtcctt ggggttggag gtgatcgtgt
     4021 tggatgccga actgaactac ccggatgcct actttgttga agatgtggct atcgtgactc
     4081 cagatgtagc ggtcatcacc aatccagggg cgccagcgcg gaaaggcgaa gaagattcta
     4141 tagagcctgt gttggcgcgc taccgcccga cagtgcgcat acagccgcct ggcacggtgg
     4201 aaggcgggga tgtgctcatg gttggaacgc attttttcat tggtatctcg gaacgcacga
     4261 atttagaggg agcaacacaa ctggggaatg tcctagagcg ctatggcaat actttcacac
     4321 tggtacatat ccaggcagga ctgcatctga aatccagtgt gaattatgcg ggcaaaaaca
     4381 ccttgctggt atgcgaggat tttgccgaga ggggggaatt tcaggggtat cgtacaattg
     4441 ttgttcccca gatagaggcc tatgctgcca acactctgtg ggccaacgat catctgatca
     4501 cgccagcggg ttttcccgaa accaggaagc ggctggtggc tctcgacctg ccgatcatcg
     4561 agctggatgt tagcgaggcg cacaagatgg atggtgggct gacttgtatg tctttgcgtt
     4621 tctagcatac aatttggttc aattcacagg ttagtcatga cggaacatct cacgccagag
     4681 aaactgcacg tcacttatat cgacgacgtc cgaaacgatg gaccgcttgc tcccagggcg
     4741 tacacattga ctcactcgga cacgacaggg gatttgtttc tcacgattgg tcagcggtat
     4801 aaccggtctc aaatatcagg tttgtatacg cggttcatgc gggacgaggt gctggctgaa
     4861 tggcggcagg aagaacagtt aaccctgcac gttcactgcc atgtcagtgg aggattggtg
     4921 ttcgggtcat ccggttaccg ggattcgatc ttcaggtacc acttgccaat ggtcttggag
     4981 gcattcaggt acggcgatca aaaattggtt gaagcacatc cccaattagc ccaggcgccg
     5041 atcatggtgc attttcatgc acgcgaggat aaatttgata aaaccgaaca gtggggggta
     5101 ttcgatgaat accagatctc agattgattt gttagtgctg tttatttccc tgtccatttc
     5161 tcttatgggc aggtaataat ttcctagaaa ggattacgat aaactatgaa agcagtccgc
     5221 atggtcgatg taaatctccc tttgcagatg tttgatatac ccatacctgc gcttggcgaa
     5281 ggcgatatct tggtacgcat tcgcgcggcg ggaatttgcc actcggatgc acattatcga
     5341 gctggaaaat cgcctgtgcg gcctctgccc atgactctgg gtcacgagat agccggtatc
     5401 gtggaagagg ttggcaaacg tgtcacatcc gtgaaagtgg gagaccgtgt ttgcttgcat
     5461 tacctgttga gttgcggcga gtgctcctat tgccgtattg gtagcgagca gttttgcacg
     5521 caaggctcca tgttggggca ttattcggat ggtggttacg ccgaatacat tgctgttcct
     5581 gagcgcaatg ccgtccatct gccggatgaa atctcgtttg agcaaggcgc aattatgatg
     5641 tgctcgtcgg caacatcatt tcatgcgctg cgcaagtcgc gactcagggc gggtgagact
     5701 gtagccatct ttggcattgg tggtttaggc atatcggcag tccagctagc atccctcttc
     5761 ggcgcgctgg aagtctttgc ggttgatatt aatccagaaa aacttaaact ggtggaaaga
     5821 tatggggcga taccgatcaa tgccacgttg aacgatccgg ttgctgagat ccgccggctt
     5881 acccacaaca aaggtgtgga tgtcgccctg gagctcatcg gactttcgca gacgatgaag
     5941 caggcagtgc gctcgctggc attcatgggc cgggcggtaa tagccgggat cagtgataag
     6001 ccgttggaga tcgataccta ttgggagctg cttgggaagg aaaccgaggt cattggcacc
     6061 aatgaccact tgctctccga gctgcccact atcatcgagt ttgcccgcca gggaaaattg
     6121 gatctatcgg gggttatcac acgcacggtc ccgctggaag ctgaccacat caaccgtgct
     6181 ttggataatc tcgaaaaatt tggtgatgat attcgaaccg tgattatatc gtgatcagcc
     6241 cggctattta ctgacccagg aaggctgatc tcatatataa ttgatctata tactatcaaa
     6301 tgaatgagca agagcagtta gaacatgcca tagcagctct agaagctcag cgctctttac
     6361 tgggggattc tgtggttgat gctgcgctgg cttcaatgcg ccagcgtcta gctattctgc
     6421 ggtcacctcc ccttgccgag cagcgtaaaa cggtcactat tctcttcgcc gacctggtcg
     6481 acttcaccgc catgtccgag caactcgacc cggaggatgt gcgcgagatc gtcaacgcat
     6541 atttcacgcg ctgtaccgaa gcgatcgaaa tgtatggggg aaacatcgaa aagttcatcg
     6601 gtgatgcggt gatggcggtc tttggcttgg ttgaagcgcg cgaagacgat cctgagaatg
     6661 ccgtgcgcgc tgccctaaac attcgccagg cgttgagtga gttgaaccag gaggtgcagg
     6721 cgcgctggga gatgcggctg cgcatgcgga tcggtatcca cactggtccg gtggtggtca
     6781 gcctgctcag tgagcgcaag ggaatggact ttgtggtggt cggcgatgct gttaacctcg
     6841 ctagccgtct gcaatcggtg gctccggcag atggtatctt gatctcccac agcacgtacc
     6901 ggcacgtgcg tgggaacttc aacgtgcagg ttatcgaacc aattcgcata aaaggcaaga
     6961 gcgagtctat tcaggcctat gtgatcctag aggcaaagag tcgtcccttc cgcttggaaa
     7021 cacggggcgt ggaaggcatc gagacgcgca tggtcggtcg tgaagccgag ttccagctac
     7081 tcagtgatgc attagaccag atggtcagac agggtcaaaa tcgtattgtc acgctagttg
     7141 gcgaagcggg tgtgggaaaa tcgcgtttga tctacgagtt catcaactgg cttgagctgc
     7201 tacctgaaaa tttcaccttt ttcaaaggcc gcgccggccc ggcaatgcaa aatgccgtct
     7261 tttctttgct gcgcgattta ttctcctttc gcttccagat ccaagacagc gacccgcctc
     7321 aggttgtgca ggagaaaatg gagcgcggca tctactgggc ttacgaaaca ctccccaaat
     7381 tagagcaagg aggagaccac ccagccaagc aacttagcat tcaagagatt cagaagcgcg
     7441 cccacttcat tgggcatctc gttggattcg actttctgga cagcccatat ttgcgagtag
     7501 ataaacatga tgctcgttat cttcgtgacc gggcgatggt ttacctggcc gattattaca
     7561 ggaccctggc ggtttaccgt ccgatcgtca ttgtgctcga agacctgcaa tgggcagatg
     7621 acaactccct ggatgtcttc tcacggatga ctagtattct cccccagtcc gactcctcgc
     7681 atggcgtttt ggttctgtgt gctgcgcgcc cggatttttt tacgcgccta cctggttggg
     7741 atgaaatcca gccgctcaat gttcgtattg acctgcaatc attggaccgc gtaacgtgta
     7801 tcgagttggt ggatgaaatc ctgcaaaaaa tcccccgggt acctgctgag cttcgcgagc
     7861 tggtcattgc cagcacagac ggtaacccgc tttacatcga agaattcatc aaaatgctca
     7921 ttgaggacca agtcattgtg aaggctgatg aggaatggca gatagatctg gcacagctca
     7981 catctattca tatccctccc acactgacgg gcattcttca agctcgactg gacagtctgc
     8041 cggtaggcga gcgattagtt ttgcaccgtg cttcagtaat tggtcgcgtc ttttgggacg
     8101 ctgccgtaga atatctggca gagaatgagc gatctgagga tatgccaata agtgcgccaa
     8161 cggctggatt actcgaccga ttgcgctctc gcgagatggt tgatcagcgt aaagaatcag
     8221 tctttgaaaa gacgcgtgaa taccttttca ggcatgccct attccgtgat gtggcgtatg
     8281 aaagtgtgct taaacgccaa cgtagcctat accatgcgca cgccgcccgc tggttggagg
     8341 cggtcaccca gcgcagcggg cgtgctgatg agttctctct cctgatcgct gaccactatg
     8401 accgcgccaa cgaagcacag ccagctgcga aatggtatta ccaggcaggt ttgcaggctg
     8461 ctgagcattt cgccaatcgc gaagcgatcc gttattttaa tcgctgcctt gagttattgg
     8521 cggatggcag tccagaggat cgtttccccg caattctggc acgcgagaag gtcttcgaat
     8581 tattgggcga gcgccagttg cagcatgaag atctggaaag cctgactcgc ctggtcgatg
     8641 aaatgacccc aggtaaagaa acagataccc gccgcgtcca ggtcctgctg cgccgctctc
     8701 gtttgagcga ggcgacgtca gataatgtcg cctccatcga gtatgctcgc cgagctttaa
     8761 agcacgctca gattgctagc ctagacgaga gtatggcgtc ttgctacctc ctgtgggggt
     8821 ccgcactttg gcgacagggt aaatatgtcg aagcccgcca gcctctcgaa aaagccctcg
     8881 agatcgctcg cagggtaagc atacccaatc tggaagcgga tatactgcgt aacctcggta
     8941 ttgtcaacgc agtgctaagt aattacaccg aagcgcgctt atactatgag caatcgctgg
     9001 cgataaaacg cgagctgggg gatttgcgcg gggagagcgc aacgctgaat agcttgagca
     9061 tgttggttta tgatttaggc gactacttgc aggcgcgcca atacctggag caaagcctga
     9121 acatcaagcg cgaaatcggc gaccgacgcg gcgaggaaat tgccatgaac aatttgggct
     9181 tggtcgctta tagtgagggc gatcctgtgg ctgcccgccg atacatggga gaggctttaa
     9241 gcatgtccca ggaaattctt gacctggagg gacagaccac agccttggca gttttggggc
     9301 tgattgccac ctatcaaggg gattatgccg ctgcgcaaga ttacctggag aactcgttga
     9361 acctcttccg tgaacaggtg aatgatgtac aaggcgagag cgaagccctc agttacatgg
     9421 ccttgcttca aaatcgccta ggaaaccagg aagtggcgca ggaatatgcg acccgcgccc
     9481 tcgaacttgc gcgcgacgtg ggagcttggc gtgaacaatt caatgccctt ctctttctcg
     9541 gtcacgcctt gctcgcgctg aaccgcacgg aagaagccat ccaggcttat cagcaggcac
     9601 tcgatctgcc cgacaaagag aggcagacca gtccacatct tgaagcggtt gccggactgg
     9661 gacgtgcctg gcttgagaaa tggcaggtga attcgggcat ttcagcaggt atggcgaacg
     9721 acgccctgga acaggcacga aactgtgtgg agattatcct tgaccacctg gcgaccaagc
     9781 ctctgcagga tgtccacgag ccagcccgca tttatctgac ctgctataac gtgttgcatg
     9841 ccagcaggga cgcgcgggca tcctgggtct tagagcaggc atatcactgg ctgaatgaca
     9901 gagccagcac ttttcaggat gaggccctga agcgcctttt cctggataac atacccgcta
     9961 accgtataat atccgctgaa tgggaaaccg ctcaccagct tgaggacaag ccatgaatag
    10021 agacttgatc cgccaggtgc ctctcttttc tcagttacca gatgcagaat tggactacct
    10081 ggcacaaacc ctacaatatt gcgagcgtgg tccagatacg atcctttttc gtgagggtga
    10141 cctatctcaa tgctttatgg cgatcttaga cggacagatc gaaattgtta aagcgctggg
    10201 cacgccagag gaaacagtgc tgaccgtgga aggtccgggt gaaatcctgg gtgagattag
    10261 ccttttcttg ccggatcgaa atcgctctgc cagcgcacgg accctaaccc atgtccagtt
    10321 gttcgagtta tctccggcag gtttccacac cctcctccat cgcaatccat cgctggcact
    10381 ccccatcatg caggtattga cccaacggtt gcgcaattca gagaatacca ccatccgcga
    10441 cctgcagcag aaaaatatcc agctctccga tgcactccaa gaattgcgcg ccgcacagga
    10501 gcaattgatt gagaaggaga agctcgagca cgagctgcag gttgcccgcc agatacagga
    10561 gcgcttcctg cctaaagagt tgccttctct acctggctgg gagatcgctg ccttctggca
    10621 gccggcgcgc gccgtgggcg gcgatttcta tgatttcgtt gtctttccca acgggcagct
    10681 tggtattgtg gtgggtgatg tgtccggaaa aggcgtcccg gctgcgctga tcatggcgac
    10741 cacgcgcagc gtgatgcgcg ctgtcaccga acatcacttt agtcctggaa gtatcctgga
    10801 gcgggtcaac gatctactga atccagatat gccgtcaaat atgtttgtca cttgtgtgat
    10861 tgccgtgcta gagccgtcca gcggtcatct gcgcctggct aatgccggtc atgatctacc
    10921 tcatcatctc gcactagatg gatcgctcga gttgcatgca actggcatgc cgctaggctt
    10981 gctgcctggg atgcgatacg atgaaatcga aaccacgctc tccgatggcg attcactgct
    11041 cttctacagc gatggattgg ttgaagcgcg cgatacgcag ggcgagatgt atggtttccc
    11101 gcgcgtgcgc aaatttctgt ctccatgggc gcaacaaaaa atggcttcgc taactcccga
    11161 cggggcgagc gaactgttga tccgcgactt actggagagt atgcaggctt ttactggccc
    11221 cacgatcgag caagacgacg acgtaacctt cgtcacttta tgtaggttgc aaatttaatt
    11281 cgggggatac acaatgccaa tctcagaaaa tgagcgcttc gattaaaggc taccaggggc
    11341 gaagcagtgc taaatttagg gtttcaaaat tcttcaagcc atttagctaa gtcccgctcc
    11401 aggctctctg cggtgtgatg aacaaatggc tctccgctgt acaacaccca ccgccaggta
    11461 tcgaaatact ccagcccgtg ataccattgc aggatttctg gatcatatcc caggcgccgg
    11521 cagtatcgtg cctctatccc ccggccgcgc agctcgggag agacaaaaaa agatagcaga
    11581 aattcaaaga tcggatcact aaatccggcg ttcgcaaaat cgatcacacc tgccaattgt
    11641 tgatcacgaa cgaggaagtt ttccaaccat aagtccccat tactaaagcg tatcgcaggg
    11701 agctcgggca tcttgtcttt caatgcagcg taggcttgtg ctgccaaggg ttgcggattc
    11761 cgaaggaaat agccctgagc ttcttccagc acatcagcgg cggtttgccg atccaatagg
    11821 tgcgcgattt ccgccaggtc gtcttgtgtg atctcctgca gggcgattac tgtgtcgaga
    11881 tacaactcct cagcccaggt attccccgcc agcattggtg ccaagagcgg ttcaccttca
    11941 ataaaatcgc taaagaagca tggcagccct agtgcttctc cctcagcatc ccacccatat
    12001 gctcgtggag ttggaattgg aatcgactgc atggcacgca ggactcgata ttcgtgctcg
    12061 atgcctctcg aatctagtcg taacacgaat gatcttaaag tgccattgca atccacatct
    12121 aagcgccacg gtgcttcccg ggtgcttttt gtcaacatct ccgcacgaag gattgttaca
    12181 ctacccc
//
LOCUS       DBNGSX010000238         7777 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1473617, whole genome shotgun sequence.
ACCESSION   DBNGSX010000238 DBNGSX010000000
VERSION     DBNGSX010000238.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7777)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7777)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7777
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1473617"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="AC16CB_07275"
     CDS             <1..725
                     /locus_tag="AC16CB_07275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3342307.1"
                     /translation="RFADHVITVSEHWRQALIERGVPAHKCSVVMNVADERIFNDSGD
                     NGAGSSDSSKFRLIYHGTIVQRYGLDLAIQAVERLRHEIPNIQLYILGKGDHIETLVK
                     MTQEMNLGDHVTIVKDMRPTEELPGIIRSAQLGIVPYRDDVFTDGLLPTKLMEYAALG
                     LPAIASRTTAIEAYFSDTMVEFFNPGDVDDLARCIRTLWNSPERLLELAQGSKNFKQR
                     YNWGKVSADYVALVDRLGTQYV"
     gene            1044..2099
                     /locus_tag="AC16CB_07280"
     CDS             1044..2099
                     /locus_tag="AC16CB_07280"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HIR3342308.1"
                     /translation="MNLQQLKIIGIDLYQDWFDAHYTILEYRSPFHHPAWMETVARSL
                     NFKTVYVGIFEKNELISVIPGFLYQRGLMRLFGSPLRGTMTSYLGATSLKPISTIQEQ
                     MGLLTACNTFLSKKLGVAYSRFTLRNEPPKRPNLTANWDDKSSGSYRLDLTSGVEELW
                     KNLKSDCRRNIRRAREAGIEITPLEDAGLFYNMIKATFSRHGSTSFHKEKFFQELLNE
                     LIPRDLLWAWGAKYEGQTIAGALFLHDDQEIHFISGASLPQFGSLPTSYLLHWKAIET
                     GTELGLRIFNSERSRIPSIDKFKESFRPVLEKRYTLINAPGYLRLAQKVYIKVYSRLR
                     NLNSRFQREKKDPQLST"
     gene            2448..3125
                     /locus_tag="AC16CB_07285"
     CDS             2448..3125
                     /locus_tag="AC16CB_07285"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HIR3342309.1"
                     /translation="MLFTELRNLSDLQDVCSILQEQGFQYEDHLNFLINLDRPLPKIW
                     NAIRSNARRNIKKAQGSGLQIRELGKPEDLHDAYGVLKAVYKRIQVPLPDLSLFRSAF
                     NVLHPKGMLKILLARLQNETIGVCCLLLYKGVAVYWYTGTLREYASYRAGDLLVWKAI
                     ELSKQEGCHTFDFGGGGKPNEEYGVRDFKAKFGGKLINYGRYVCIHNPMRLKISQAGY
                     RMIRRFL"
     gene            3122..4207
                     /locus_tag="AC16CB_07290"
     CDS             3122..4207
                     /locus_tag="AC16CB_07290"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR3342310.1"
                     /translation="MNAPNNEKSSYIDTLNERPIFVVGAPRSGTTLLRYILCSHPHIY
                     IPPESNFIPRFFNKQPETQLQPLEAKRIYQEILGYRVFFKDWHTEKPDPETFIFNLPD
                     LYPRTILTALYKMYSQQYSSERWGDKSPIYTEHLALLSQIFPSAQIIHIIRDGRDVAL
                     SMLRSYRGSKFFYIDVCYAAHSWKRRVRQARSYGETLGIDRYHEIRYEELVANPTEVL
                     SAICNFLGEAYEPSMAEPYKEAKAHFHSKGIHAATRQPLTTKSVGRWQSEMSEADQRI
                     FQAIASDLMNELGYQTAELGKMSLAEKARFAQLHAKYYMVDVSRRTLQASGIFHPTSV
                     IRSIKLPYPINRYRSSNKKNGRNLEKA"
     gene            4449..5441
                     /locus_tag="AC16CB_07295"
     CDS             4449..5441
                     /locus_tag="AC16CB_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3342311.1"
                     /translation="MKILIASSIDSDAIEQLKQHHKVILAFNKPNEPLETLIPDCEVL
                     VFRSGVKISAQLMESAPNLKLLIRAGSGLDNLDLDYVQRNGLVLIRIPQPGAKAVAEM
                     TFALMLALSRRLFEADQSMRQGRWKKYELSGYLLQGKTLGIIGIGEIGGRVAEMGMAW
                     GMKAISHDGFLSPERARLLSEKGVELLDFDQVVSQSDYLSLHVPLNESTRYMINASVL
                     ENMKPGSYLINLARGGIVDEQALYKALTSGGRLRGAALDVHEKEGEGKLSPLASLNNV
                     ILTPHIGAMAVDAQREIGQRVVEIVKSYTASPNQVKVPRNGGVVIESDSQTVRI"
     gene            5444..6340
                     /gene="aepX"
                     /locus_tag="AC16CB_07300"
     CDS             5444..6340
                     /gene="aepX"
                     /locus_tag="AC16CB_07300"
                     /EC_number="5.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050188 - phosphoenolpyruvate mutase
                     activity [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate mutase"
                     /protein_id="HIR3342312.1"
                     /translation="MTRKTTQFKQLLNSPQVEFLMEAHNGISARIVEEAGFKGIWGSG
                     LCISAAMGVRDNNEASWTQVLEVLEFMSDATTIPILVDADTGYGNFNNVRRLVQKLEQ
                     RSVAAMCIEDKLFPKTNSFIKGELQPLAEIGEFCGKIKAAKDIQQDPDFCVVARVEAL
                     ITGQGLGEALRRAEAYYEAGADAILMHSKKSTPDEVLAFMKEWKDTCPVVIVPTTYFS
                     TPTQVFAEAGVSVVIWANQLMRASIAAMQQTAAKLYQEQSLLEVETQIVKVKEVFRLQ
                     NADELNEAELRYLPMNGVHHNS"
     gene            6465..7097
                     /locus_tag="AC16CB_07305"
     CDS             6465..7097
                     /locus_tag="AC16CB_07305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3342313.1"
                     /translation="MRAFTKGGVTLKERHLSYCPRCNSKARHRRIWLFLKDKTNLFSD
                     RLRLLHVSPKYSLSRRFIRMPNLDYVGVDLNERPNISVKMDLTATPIRSAAFDAVICV
                     HVLEEILDDRKAMRELYRVMKSGGWALISVPTRMEQLTYEDPMIVTPDERQRAFGEVA
                     HVRIYGHDLVDRLKECGFQVQVDLAKDVPQQVREKYGLRDDENIFYCLKP"
     gene            7199..>7777
                     /locus_tag="AC16CB_07310"
     CDS             7199..>7777
                     /locus_tag="AC16CB_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HIR3342314.1"
                     /translation="MFTVALIGGDGAGKTTIAKQLEKSLPWPNRYLYMGQSTISSNAA
                     LPTSQVAKILKLRLDKRNGAQSGNSNPKAPSSNDLHYGSAKRSAIWVTARFMNRLAEA
                     WWRQLLTSYYLLRGYVVIYDRHLLFESAPLNNSKIKRKNLLDHVEHRIMKYFYPKPNL
                     VIFLDAPPEVLYNRKGEASPQYLEKRRETTLEQ"
BASE COUNT         2207 a         1778 c         1857 g         1935 t
ORIGIN      
        1 gccgctttgc tgaccatgtg attactgtca gtgaacactg gcgacaggcg ctaatcgagc
       61 gaggtgtgcc tgcacataaa tgcagcgtgg tgatgaatgt cgccgatgag cgcattttca
      121 atgattcagg agacaatgga gcgggctcgt cagacagctc aaaattccgg ttgatctatc
      181 atggaactat tgtccaacga tacggtctgg acctggcaat ccaggccgtc gaacgcctaa
      241 gacacgagat ccccaatatt cagctctata tactaggcaa gggagatcac atagagacgc
      301 tggtcaaaat gacccaggaa atgaacttag gtgatcatgt gacgatcgtg aaagacatgc
      361 gtcccaccga ggaactgccc gggattatcc gttctgcgca gttgggaatc gtcccttacc
      421 gggatgacgt ttttacggat ggattgctac ctaccaagct gatggagtat gccgctcttg
      481 gattacccgc tatcgcctca cgcactacgg ctattgaagc gtacttcagc gacacgatgg
      541 tcgagttttt caatccgggg gatgtagatg acctggcaag atgtatccgc acgctctgga
      601 attctcccga gcggctgctg gaattggccc aaggaagcaa gaatttcaag cagcgctata
      661 actggggcaa agtcagcgcc gattatgtgg ctctggtgga tagattggga actcaatacg
      721 tctagatttt ttgtatccat taatcttgaa aagacatctg aacgagtaac aaaccggaca
      781 aaatatccgt tcatataata gatgaagtaa ttggatatgg acttttgatg atagtcctta
      841 tcaccggcag catctatgcg gaggtcattg aacaactaca agttatcttg caacgtatca
      901 taaatctcgg cttaatcaca ggaaactttt acggggagga atgcaaacaa atcgtgctca
      961 acccgggcta tatagcaggc attgctattt ccttccaatg aggaaccgtt agagatatat
     1021 gaataccctg ggagccaaag tgtatgaact tacagcaact aaagattatt ggaattgatc
     1081 tatatcaaga ctggtttgat gctcactata cgatacttga atatcgatca ccctttcatc
     1141 atccagcatg gatggagacc gtcgcgcgta gtttaaactt caagaccgtt tatgtcggta
     1201 tatttgaaaa gaacgagtta atctcggtaa tccctggttt cttataccag cgcggtctga
     1261 tgcgcttgtt cggatcacct ctaaggggga caatgacttc ctatttgggt gcaaccagcc
     1321 tcaaaccaat ctcaacaatt caggaacaga tgggcttgct cacagcttgc aatacgtttt
     1381 taagcaagaa gctgggggta gcttacagcc gttttacact gcgcaacgaa ccacctaaac
     1441 gaccaaacct aaccgccaat tgggatgata aatcatctgg cagttaccgt ctcgatctga
     1501 caagtggcgt cgaagaattg tggaaaaacc tgaagtcaga ttgcaggcgg aatatccgca
     1561 gggcgcggga ggcgggtatc gaaattactc ccctcgagga tgcaggcctg ttctacaaca
     1621 tgatcaaagc aactttctcg cgacacggtt caaccagttt ccacaaagaa aaattctttc
     1681 aagagctttt aaacgaactt atccctcgtg atttgctatg ggcgtgggga gcaaagtatg
     1741 aggggcagac catcgcagga gctctcttct tacatgacga tcaagagata catttcatca
     1801 gtggagcatc tctgccacaa ttcggatcgt taccgacgag ttacctgctt cactggaagg
     1861 cgatcgaaac aggtacagaa ctgggattgc gaattttcaa ctccgagagg agtcgcattc
     1921 ctagcattga taagtttaag gaatccttcc gaccagtatt agagaaacga tatacattaa
     1981 ttaacgctcc aggttacttg cgtttagcac aaaaagtcta tattaaggta tattctcgcc
     2041 ttcgaaatct caacagccgt tttcaacgag aaaagaagga cccccagtta tctacctgat
     2101 aacgcgcgca taacaactat atgaaaatag tgcagcaact agatgagaat ctgtggttag
     2161 attttttagc caatcacccg caggcaaata tcttccacac accggaaatg caccaggtat
     2221 ttacgcaggc taaaggtcat caccctacac tgtggagtat tacatctgat gacaatcagg
     2281 tttacgcgtt gttcttgcct gttcaaatta ccctgtacaa tggacccctt cgaactttta
     2341 catcacgggc agtatcttat ggaagtgtgt tgttccttcc caatcccaaa gggtatgctg
     2401 ccctgggtaa gctgctgagg tcctacgcgc agaaggcaaa acacagcgtg ttatttacag
     2461 aattaagaaa cctatccgat cttcaggatg tctgttcaat attgcaggag caaggttttc
     2521 aatacgaaga ccacttgaat ttcctcatta acttggatcg accccttcca aagatttgga
     2581 atgcgattcg ctccaatgcg cgccggaata tcaagaaggc acaaggctca ggtttgcaaa
     2641 ttcgggagct tggaaaaccc gaggatttgc atgatgcata tggtgtgctt aaagcagtat
     2701 acaaacgcat acaagtgcct ctgccagatc tttccctctt tcgctcggca ttcaatgtat
     2761 tacacccgaa aggcatgcta aaaatattgt tggcaaggct tcaaaatgaa actatagggg
     2821 tgtgctgctt gctgctatac aaaggggtgg ccgtctactg gtacaccgga acactacgag
     2881 agtacgcatc atatcgggct ggcgatctac tcgtctggaa ggcgatagaa ttaagtaagc
     2941 aagaggggtg tcacactttt gatttcggtg gcgggggcaa accaaatgaa gaatatgggg
     3001 tgcgtgattt taaagctaaa ttcggtggca agttgattaa ttacggtcga tacgtttgca
     3061 tccataaccc aatgaggtta aagattagcc aagctggcta ccgaatgata cgaaggttcc
     3121 tgtgaacgct ccgaacaatg aaaagagtag ttatatagac acacttaatg agcgtccgat
     3181 atttgtagtc ggtgcccctc gttcgggcac aactctgctg cgttatattc tttgtagcca
     3241 tccacacatt tatatcccgc ccgaatccaa cttcatccct agattcttca acaaacaacc
     3301 cgagacacaa ctgcaaccat tagaggcgaa acgcatttac caggaaatac tgggttatcg
     3361 cgtcttcttc aaggattggc acacagagaa accagatcct gagactttca ttttcaattt
     3421 acctgattta tacccaagaa ccatattaac tgcattgtat aaaatgtaca gccagcagta
     3481 tagttcagaa cgttggggtg ataaatcacc tatatatacc gaacacctag cattgctttc
     3541 ccagattttt ccctctgccc aaattattca catcattcgc gatgggcgtg atgttgctct
     3601 ttcaatgcta cgctcgtatc gaggctcaaa attcttttac attgatgtgt gctacgctgc
     3661 tcatagttgg aagaggcgcg tccgacaagc aaggtcttat ggtgaaacct tgggaattga
     3721 tcgctatcac gaaattcgct atgaagaatt agttgccaac ccgactgagg tgttgtcagc
     3781 gatctgtaat tttctgggtg aggcatacga gccgtctatg gctgagcctt acaaagaagc
     3841 gaaagcgcac tttcattcaa agggaattca cgcggctacg cgacaacctt taactactaa
     3901 aagcgtggga cgctggcaga gtgagatgtc tgaggcagac cagcgcatat tccaggcaat
     3961 agccagcgat ctgatgaacg agttaggcta ccaaaccgct gagttgggaa agatgagctt
     4021 agctgagaaa gcccgtttcg cacagctgca tgctaagtac tacatggtgg atgtaagccg
     4081 acgcacgcta caagcatctg gtattttcca ccccacctca gtgataagat ctataaaatt
     4141 gccctatccg ataaatagat atcgttcatc caataaaaaa aatgggcgaa atcttgaaaa
     4201 agcctgattc ccttttctaa tctatgctct cttgccggga taatacatat agggggttaa
     4261 ggaacaagct ctcatcctgt aactgagtgt tggataacca ccatcaaatt gcctacgctg
     4321 ccctaaacca aacttccgtt agatatttac acttccatca atcttgaaag cctgccagcg
     4381 gctagtaaca agctgagcaa gatttcgttc atattgatac ctaagcgctt atcggagatt
     4441 atcacatcat gaaaattctt atcgctagct ctattgatag tgacgccatt gaacaactta
     4501 aacaacacca taaagtcatc ttagctttta acaaaccgaa cgagccactc gaaacgctta
     4561 tccctgactg cgaagttttg gtcttccgca gtggtgtaaa aattagtgcg caacttatgg
     4621 aaagcgcacc caatctaaag ctgctcattc gcgcaggatc tggactggac aacctggacc
     4681 ttgattatgt gcagcggaat ggattggtgc ttatccgcat accacagcct ggggctaagg
     4741 cggttgcaga gatgacgttt gccttgatgc tggcgctcag tcgcaggctt ttcgaggccg
     4801 atcaatccat gagacagggt cgctggaaaa aatacgagct atctggatac cttttacaag
     4861 gtaaaacact gggaattatt ggaattggag aaattggcgg ccgcgtcgct gagatgggga
     4921 tggcctgggg catgaaagcg atcagccatg atggattcct ctccccagaa cgggcgcgcc
     4981 tgctcagcga gaagggtgtc gagttgttgg attttgacca agtcgtatct caatcggact
     5041 atctcagcct gcatgtgccg ctcaatgaat ccacaagata tatgatcaat gcctcagtcc
     5101 ttgagaacat gaagcccggc tcttatctaa tcaatctagc ccgcggtggc atcgtcgatg
     5161 aacaggcatt gtataaggca ttgaccagcg gaggcagatt gaggggggca gccctggatg
     5221 tccatgagaa agaaggcgaa ggcaaattat cacccctggc tagcctgaac aatgtgatcc
     5281 tcacccctca tattggcgcc atggcagtcg atgcgcaacg tgagattggg cagcgggtgg
     5341 ttgagattgt caaatcctac acagcaagcc caaaccaagt aaaagtcccg cgaaatgggg
     5401 gggtggttat tgaaagcgat tctcaaacag tgaggatttg aacatgacca ggaaaacgac
     5461 ccaatttaaa cagctattga actctcctca agttgagttt ctgatggaag cgcataatgg
     5521 cataagcgcc aggatcgttg aagaagcagg ttttaagggt atctggggaa gcggactatg
     5581 catttccgct gccatgggtg tgcgagataa taatgaagcc agctggacac aggtgctcga
     5641 ggtccttgag tttatgagtg atgcgaccac gatccccatc ctggttgatg cagatacggg
     5701 ctatgggaat tttaacaacg tgcgcaggct ggtccagaag ctcgaacagc gcagcgtagc
     5761 tgctatgtgc atcgaggata agctcttccc taaaaccaac tctttcatca aaggtgaact
     5821 gcaaccgtta gcggaaattg gcgagttttg cggcaagata aaagcagcaa aagacataca
     5881 acaggaccct gatttttgcg ttgtggcacg ggtcgaggca cttatcactg ggcaggggtt
     5941 gggggaagcc ctcagacggg ctgaagcgta ctatgaagcc ggggcagacg ccattcttat
     6001 gcacagcaag aaatcaactc ccgacgaagt gctagccttt atgaaggaat ggaaggatac
     6061 ctgcccagtt gtcatcgtcc caacaaccta tttcagcacc ccaacccagg tatttgccga
     6121 agccggagta agcgtggtca tttgggccaa tcaactgatg cgagccagca ttgcagcgat
     6181 gcagcaaaca gccgccaagc tgtatcagga gcaatctctt ttggaggtgg agacacagat
     6241 tgttaaggtt aaggaagtct ttcggttgca aaacgcagat gaattgaatg aggcggaact
     6301 cagataccta ccgatgaatg gggttcacca taatagttga gatgagaaac acctatcgga
     6361 ttgctgacct gtccttgccc cttcattggg ctgaggtact actcgctttc cgtgctttac
     6421 tctttgtcgg cacaaaatac acctgcccct gctgtggatg gagggtgcgt gccttcacca
     6481 agggaggtgt tacgctcaag gagcgtcacc tcagctactg cccgcgctgc aattcaaaag
     6541 ccaggcatcg tcgaatatgg ttatttctaa aggataaaac aaacttgttt tcggatcgtt
     6601 tgcgattatt gcatgtatcg cccaaataca gtttatcccg acgctttatc agaatgccaa
     6661 acctcgatta cgtcggtgtt gatcttaacg aacgtccaaa tatatccgtg aaaatggacc
     6721 tgactgcgac cccaatccgt tctgcggcat tcgatgcggt gatctgcgtg catgtcttag
     6781 aagagatcct ggatgaccgt aaagcgatga gagaactcta tcgggtcatg aaatctgggg
     6841 gctgggcgct cattagtgta cctactcgca tggaacaatt aacgtatgaa gatccaatga
     6901 tcgttacacc agacgagcgg cagcgtgcat ttggtgaagt cgctcatgtc aggatttatg
     6961 ggcatgactt agtcgaccga ctaaaagaat gcggtttcca ggttcaagta gatttggcaa
     7021 aagatgtgcc ccaacaggtc cgcgaaaagt atggcttacg tgatgacgag aatatcttct
     7081 attgtttgaa accgtgatca agatgtactg ttagatttaa gaattacgca gctgaaggaa
     7141 taagatcgtc aactgcggaa cttctaagat taatgtttgg aactagtaag aggtatttat
     7201 gtttaccgtt gctttaatag gaggagatgg cgctggaaaa acgactattg ccaagcagct
     7261 tgaaaagtcc cttccatggc caaatcggta cttgtatatg ggtcagagta ccatatccag
     7321 caacgcggct ttgcccacat cacaagtagc aaaaatcctg aaactgcgac tagacaaaag
     7381 aaatggcgct caatctggga acagcaaccc caaagcgcca tcctctaatg acttacacta
     7441 cggatcagca aaacggagcg cgatatgggt cacagcgcgt ttcatgaacc gtttggcaga
     7501 agcctggtgg cgtcaactgc tcacttcata ttacctactt cggggctatg tggtgatata
     7561 cgaccgtcat ttactgtttg agtctgcccc tctgaataat tcaaagatca agaggaaaaa
     7621 cctgctagac cacgttgaac atcggataat gaaatatttt tatccaaaac caaacctggt
     7681 aatttttttg gacgcacccc cagaagtgct atacaatcgc aaaggggaag catcacccca
     7741 atacctggaa aaacgcagag aaactactct agaacag
//
LOCUS       DBNGSX010000239         3137 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_157384, whole genome shotgun sequence.
ACCESSION   DBNGSX010000239 DBNGSX010000000
VERSION     DBNGSX010000239.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_157384"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2060
                     /locus_tag="AC16CB_07315"
     CDS             <1..2060
                     /locus_tag="AC16CB_07315"
                     /inference="COORDINATES: protein motif:HMM:NF016330.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HIR3342315.1"
                     /translation="VGRFAQCCACRSQDLTWFVAILGLNMQPLPFGFWLKSQSPAHFL
                     SSVVGNRPNAIALGHPLLCFAAGLDYNIVGHNLCFYQGATVMKGPTILTENMLRSLST
                     ENSFERGRQYHRAGAIRNATRQGNNLKALCEGSSAPAYRLRVELDQVGIRSAYCTCPY
                     DWGGLCKHLIALLLTYIHQPEAFSERGSVADILAGLERDDLAALIEKLTSRDPDLYDW
                     LEMSISQLRPKKEGTPLESSATSGSRKDKRETMVSEQTYRRQVKNILHSLDGLSSSQA
                     YWGVSGMVERLEEVHQSAVQFLEAGDSKGTIKILMVLLQEVAEEYEFFDDSNGELGDF
                     LDSLGLPLAEAILSFEMETGDRNKLEESIKDIWHHLSDYGIDGLDVAAAALKYGWEGA
                     EPGFDEDNWQDDKEWDENDWDGYVIDELVTARLNVLERQGSTNEYLEQCLQSGDYLRY
                     TLKLLEIGRTDEAASTAINQLADAEHALIAAQRLRELDRIEDALKIAERGFSLAGRKY
                     DLGCWLGPLQEARGAIDQAILAYRAAFEEMPSLELYQTLKRISGHRWETIHSQLMDIL
                     KSSTNTHALVDTYLFEGRWDAATRLADQGGTWDYHLVEKVADAVVTERPDWVIQACRL
                     QAEGLIERTQSKYYPHAARWLARMKQAYLQKGEPAEWEAYLSNLKNTYARRPALQAEL
                     KRL"
     gene            complement(2466..2537)
                     /locus_tag="AC16CB_07320"
     tRNA            complement(2466..2537)
                     /locus_tag="AC16CB_07320"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2503..2505),aa:Lys,seq:ttt)
     gene            complement(2593..>3137)
                     /locus_tag="AC16CB_07325"
     CDS             complement(2593..>3137)
                     /locus_tag="AC16CB_07325"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3342316.1"
                     /translation="EVTALVAEKYKKRPFFRWIIRLVDGIWINRGEADLHALRQARDH
                     LRSGGVLGIAPEGTRSHTGALMRGKTGVAYLADKAGVPVIPTAISGTDGAVWQLLRFR
                     RPAIQIQFGAPLTFPPIQRHEREATLQSYTDEIMCQIAAMLPEKYRGFYADHPRLKEL
                     LEAMPHSRASGEGQAVSAER"
BASE COUNT          737 a          823 c          880 g          697 t
ORIGIN      
        1 tggttggccg gtttgcacaa tgctgtgcat gccgcagcca ggatttgacc tggtttgtcg
       61 ctattttggg gctcaatatg caacctttac ctttcggatt ttggttaaag agccaaagtc
      121 cagcccattt tctctcctca gtggtgggaa atcgcccaaa tgcgattgcc ctagggcatc
      181 cactgttatg cttcgctgct ggtctagact ataatattgt cggtcataat ctttgtttct
      241 atcagggagc aacagtgatg aaaggaccga ccatattgac agagaacatg ctgcgctcgc
      301 tcagcacaga aaatagcttc gagcgaggca ggcagtatca ccgcgccgga gccatccgta
      361 acgccacccg ccaaggtaat aatttgaagg cgctgtgtga aggcagcagt gccccagcat
      421 accggctgcg tgtggagctg gatcaggtag ggattcgttc tgcatattgc acctgcccgt
      481 atgattgggg cggactatgc aagcacttga tcgctctgct gctgacctac atccatcaac
      541 ccgaggcttt ctccgagcga ggatctgttg cagatatact cgccggtctg gaacgcgacg
      601 atctggcagc tttgatagaa aagctgacct ctcgcgaccc cgacttatat gattggctgg
      661 aaatgtccat atcccaactt agaccgaaaa aggaaggcac tcctctggag tcgtccgcta
      721 cctctggctc cagaaaagat aagcgcgaga ccatggtctc tgagcaaacc taccgccgtc
      781 aggtcaagaa tattctgcac agcctggacg gcttgtcttc ttctcaggct tattggggtg
      841 tgagcggcat ggtggagcgg ctcgaggaag tgcaccagtc cgccgtacag tttctggaag
      901 ccggcgactc caagggtacc atcaaaatct tgatggtgct cttgcaggag gttgcggaag
      961 aatatgagtt cttcgatgac tcgaatggag aactgggcga ctttctggac agcctgggac
     1021 tgcccctggc tgaagcgatc ctgagcttcg aaatggaaac tggtgatcga aataagttag
     1081 aagagagcat aaaggatatc tggcaccatc tctccgacta tggcatcgat ggcctggatg
     1141 tggcagcagc cgccttgaaa tacgggtggg agggtgcaga acctggtttc gatgaggata
     1201 actggcagga tgacaaagag tgggatgaaa acgattggga cggatatgta atcgacgaat
     1261 tggtcaccgc ccgcttgaat gtgctggagc gccagggcag cacgaatgag tatctagaac
     1321 aatgcttgca gtccggcgat tacctccgct acacgctcaa gctgctggag ataggccgca
     1381 ctgacgaggc agccagtaca gctatcaacc agttggctga tgcggagcat gccttaattg
     1441 cagcccaaag gctgcgagaa ctcgatcgca tcgaagatgc cctgaagatt gccgaacgag
     1501 ggttttccct cgcaggccgc aagtacgatc ttggctgctg gctgggaccc ctccaagaag
     1561 ctcggggggc catcgaccag gccatattgg cttatcgggc agcctttgaa gaaatgccct
     1621 ccctggagct gtaccagact ttgaagcgga tatccggaca tcgatgggag actatccact
     1681 cacaactcat ggatatcctg aaatcctcta caaacaccca cgctctggta gatacgtatc
     1741 ttttcgaagg gcggtgggat gcagccactc gcctggccga tcaagggggc acctgggatt
     1801 atcacctagt tgagaaagta gctgacgcgg tggtcaccga gcggcccgac tgggtgatcc
     1861 aggcttgccg cctgcaagcc gagggtctga tcgagcggac acagagtaag tactatccac
     1921 atgccgccag atggctggcc agaatgaaac aggcctactt gcagaaggga gaacccgccg
     1981 aatgggaggc ctatctgtca aatctgaaga acacttacgc gcgcaggcca gccttgcagg
     2041 cggaactaaa gaggctgtga tgtagcaaag ctctctcgat ctcatcgtag tcaacctgca
     2101 ttcaatggtg acaaattgaa taggataggt ctcagccaat ggttgatctg atgcattatt
     2161 gcggccaaaa cgatttccaa ccttctaatg tccacccatc cactccctag tggattatgg
     2221 taaatgcaat ctgtatcgtg cgctttcaga tatccgtacg atgtaaacgg gatctcaaac
     2281 aaaacaatgt atagggtgag ctttatgttc gctgacaact ggtgttgact gatgttgaca
     2341 agcatataag ttcagccggt caagtacatt ggacaaaata tcattttcca aggttcaaac
     2401 catggtttta attgatcaac tcgaagattc ccaagcaaaa cccgccagac gacgggttct
     2461 cgaagtgagc gcgcaggggc tcgaaccctg aaccaatggc ttaaaaggcc actgctctac
     2521 cattgagcta cgcgcccaag tgatcgccgg atagcccggc gcattgcatt ctatcatgcc
     2581 ccatagcggg cgtcaacgct ccgcgctcac cgcctgccct tcaccgctcg cccggctgtg
     2641 gggcatggct tccagtaatt ctttcaaccg agggtggtcg gcgtagaaac cgcgatactt
     2701 ctcgggtagc atcgcggcga tctggcacat gatttcgtcg gtgtagctct gcaaggtggc
     2761 ctcgcgctcg tgtcgctgga tgggggggaa ggtgagaggt gcgccgaact ggatctgtat
     2821 ggctggtcgt cgaaagcgca gcaattgcca gacggcgcca tcggtcccgc taattgcagt
     2881 tgggatcacc ggcacgccgg ctttatcagc caggtaagct accccggtct tgccgcgcat
     2941 cagggcgccg gtgtggctgc gcgtcccctc cggggcgata cccaatacgc cgccacttcg
     3001 cagatggtcg cgcgcctggc gcaaggcatg caggtcggct tcgccccggt tgatccagat
     3061 gccgtctacg aggcgaatga tccagcgaaa gaacgggcgt ttcttgtatt tttcagccac
     3121 cagggcagtg acttcat
//
LOCUS       DBNGSX010000240        15176 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_504441, whole genome shotgun sequence.
ACCESSION   DBNGSX010000240 DBNGSX010000000
VERSION     DBNGSX010000240.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 15176)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15176)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15176
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_504441"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..1599
                     /locus_tag="AC16CB_07330"
     CDS             139..1599
                     /locus_tag="AC16CB_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HIR3342317.1"
                     /translation="MPIHFGTDGWRAVISDTFTYTNLRMVAQAIADAISSAQWNNETL
                     DNGNPDSRDIIVGFDTRFLSDRYAGEVARVLAANGYTVHLAQADAPTPAISYNLKHLG
                     ATAGIMITASHNAPRYNGVKLKAAYGGSALSEQCRRVEVYLNDNEERARGPNLMDYEQ
                     ARQLGLIRRFNPLPAYYDHLRKLIDFDLIAENPQRVVVDSMYGSGRGVLKGVLQGTGC
                     EVQEIRGEMNPGFGGVHPEPIAKYLGALAGAISTHMGNFGLATDGDADRIGAMDERGN
                     FVDPHKIMALGLRHLVERRGWRGPVVRTVSTTRMIDRLAKQYGVPVYETPVGFNHIAN
                     YMLKEDVLIGGEESGGISFRGHIPEGDGILMGLLIIEMVAASGGSLYELVEELLRQAG
                     PIQYQRNDLRLKQPVPKDRMNQRLVENAPAQIAGENVLEVSTLDGVKYILADDSWLLI
                     RPSGTEPVLRVYAEGRTAEMVRSMIGFGEQVAASFT"
     gene            complement(1603..2142)
                     /locus_tag="AC16CB_07335"
     CDS             complement(1603..2142)
                     /locus_tag="AC16CB_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIR3342318.1"
                     /translation="MEELDTNNIEFPGEEIPPPEESSNRTFMIVAGILGGITVLALAC
                     IAAYAFLVLPRQRAQQEAQIATLTAQANEVAQALTSTAIAAAIVPTSTNTPLPTATLA
                     RTATPLLAMATNTPVPSSDPRTATVAALLTQAAAVTQATSTALPSTGFADEVGMPGLL
                     GMAVLLIVVIFLARRLRTA"
     gene            complement(2191..3246)
                     /locus_tag="AC16CB_07340"
     CDS             complement(2191..3246)
                     /locus_tag="AC16CB_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342319.1"
                     /translation="MLAPVVHILPLTRIRRDRLLPVPGKVLVRRGQMVSTTDVIAESN
                     LNPEHLLLNIARGLGLPEEKADQYLQCKAGMRVSQGDVLAGPVGMAKRVVRAPKNGNV
                     IVAGGGQVLLELESPPYEVKAGMPGTVVHLMDELGAAIETTGALIQGVWGNGRLDFGL
                     MYVLMKNPDDVFTPDRLDVSLRGSIILGGYCDNPEVLKSAASLPMRAMIFSSMAAALI
                     PLAERIRIPILVIEGFGQHPMNSAAFKLLSTNERREVAVNAQPWDPFTGNRPEIVIPL
                     PANVDPPLPQDTEPFTAGQQVRVIRAPHQGSIGTIVELRPGMSELPNGISAAVAEIQL
                     ESGEKALLPLANLEVLA"
     gene            complement(3323..5077)
                     /locus_tag="AC16CB_07345"
     CDS             complement(3323..5077)
                     /locus_tag="AC16CB_07345"
                     /inference="COORDINATES: protein motif:HMM:NF025309.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HIR3342320.1"
                     /translation="MSTSLIDADSVLAIDIGSITTRAALFDVVDGRYRFLASGSAQST
                     INAPYNDISEGLRTALDRLQTVTGRILIDADERLIIPSTSEGAGVDIVAATLSAGPPL
                     NVVVIGLLEDISLESAKRVVYTSYAKIVDTLSLNDRRTVEERLDAIIRLRPDLVIVAG
                     GTEGGASQSVLRLVEAVSLAAHMLPQGHRPQILFAGNQSLRDEIKATFDKLAPLQFAT
                     NVRPTLENEQLESAQLHLAHIARQVRSQQIIGVTELDNWSGGGLLPTSTAFSRVIRFL
                     SKVYDSTKGVLGVDVGSSATSIAASFEGNLVNRVYPQLGLGHGLKEFLNVSPLQGITR
                     WLHIGLSDDDVRHYIHNKALYPASLPVTPEELAIEQALARQAMQISIKKTSNGFPEKV
                     MRYGPDLSPWFEPIVAMGSVLTRAPNLPQTMLMLLDGLQPTGVTTLVLDQNHLAPSLG
                     AVAAINPLMVVQVLESNTFLTLGSVISPVGVARPGTPILRIRMIYEGGAETSLEVKQG
                     SIEMLQLPTGQTAQLHIQPLHRFDVGMGGPGRGGRLRVVGGALGVVIDARGRPLYLPN
                     DPALRRELIAKWRWMVGC"
     gene            complement(5087..5785)
                     /locus_tag="AC16CB_07350"
     CDS             complement(5087..5785)
                     /locus_tag="AC16CB_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342321.1"
                     /translation="MSLADLSLTDMLGSIIGFFLTLMVFSYAFGDNAMFRTAIHIFIG
                     VASGYAAVVAWYNVIWPQLIIPMVFGTQADRIDILFPSVLGVLLLFKASPRLSPLGNP
                     AVAYLVGVGAATAIGGAILGTIFPQSLASINLFDQQNLQQGGEYVIQLAKGSVVLIGT
                     LATLAYFHFGARQYPNQPAGRPKWLEGLAWIGQIFIAIALGSLFAGIFAATLTALIER
                     VDFIIDFIFALIPS"
     gene            complement(5782..8235)
                     /locus_tag="AC16CB_07355"
     CDS             complement(5782..8235)
                     /locus_tag="AC16CB_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ICP22 family protein"
                     /protein_id="HIR3342322.1"
                     /translation="MDEVRCPMCGKLNPEELDTCQYCQARLKPLNISSAPEEDAEITS
                     SSASFNETPSQKKPPDQTPALPEWLQPDWLQSLRQGSGTDELGEIEPEDQLPDWSSEE
                     VDPGDQETESAESSAPEDSSEKPDWLEGLRAANQAEPETPSEQDSESTYSEEMPEDEI
                     PDWLQRIRARQSSDEPELPLEEPDAESAEFLAKLRMSPDADETDSSEQADAEEAQPGP
                     DEEDSQVPDWLAEYAESGTESTPPELPDWLKSDELPAEPEDEEDSLAEFPSRMTPSQP
                     PFTEPFLEEDIELESATELPAETPPDKPPFTEPVDEEEAEGEIADWLADLEIPAEQEE
                     MTSLPVEDEEKTSFAFEAEPVEDQPDWLQDIEIKDGISPKEDEQDQDSELEVVAPFTL
                     DEESADLIQEELPDWIKEIPAAEAQTEAEPSEEAAGELEPAELPTWLESMRPIESVAP
                     SAPASDELDQIVESSGPLAGLQGILPAEPEIAREMKSPVYTVKLQVSENQQSLASRLA
                     EMVKSEGIAAPIPRAPTISSQAVVRITLFLILLVSVVWPLYTGDLGAPAPELSPEVLD
                     AQLLINALPDGGNVLLAIDYQPAYSGEMNATTASLLDHLMIKGAYLTLVSTSTTGPAQ
                     AEQLVRYVNNQMGHQYRDTSQYANLGYIGGGLSGLRSFAQSPQQIMPYALNASTADES
                     TVWINGRLSNIQALSDFALTVVISDSPETARNWIEQVGPALEDTPLLLVVSAQAEPLV
                     RPYYEGYPKQAKGLVVGLSGGVAYESSMPRPGIARRYWDAYSYGIPTAVFLILIGGLV
                     NIVLAYLPTLKPGQGRAKR"
     gene            complement(8238..8867)
                     /locus_tag="AC16CB_07360"
     CDS             complement(8238..8867)
                     /locus_tag="AC16CB_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342323.1"
                     /translation="MKIKVPFSAAIAILFGLIVLAGYFIEIPWLSDLHNMFLQWAVIL
                     AAVALLVGIANLASVHWTKASSEQNGSFYSAVLIISLLITLIVVGYLGPTAEWSLWIF
                     NNIQVPVESSLLAILVVVLTYAAMRLIRRRLNLFSIIFIGTGLITLLGIAPLFGVVIP
                     GLHGAFGFRTLIAQIPAVAGARGILLGVALGTVATGLRILMGADRPYRG"
     gene            complement(8864..9628)
                     /locus_tag="AC16CB_07365"
     CDS             complement(8864..9628)
                     /locus_tag="AC16CB_07365"
                     /inference="COORDINATES: protein motif:HMM:NF042826.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HIR3342324.1"
                     /translation="MIPPNLFGIGQMGLFFVILFFIILVIFAFFGRRRPPAALREIPA
                     FQKLWRAVGLAVEAGTRLHLTLGRGNLSGLQSGSALIGLSILDRIARTASISDRPPIA
                     TSGEGTLGILSQDTLYNSFRAVGQGGQYDASLGRISGLTPFAYAAGTLPAIYDEHVST
                     TILSGHFGSEVALITDASERVGATTLAGSDEVSAQAVLYAAAQEPLIGEELYAAGAYL
                     KAGLFHNASLRAQDVLRWVLILAILLGAILKFLQIL"
     gene            10290..13901
                     /gene="nifJ"
                     /locus_tag="AC16CB_07370"
     CDS             10290..13901
                     /gene="nifJ"
                     /locus_tag="AC16CB_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HIR3342325.1"
                     /translation="MKRPMVTIDGNEATAFVAHRVSEVIAIYPITPSSSMGEHADKWS
                     AINHKNIWGTVPLVVEMQSEGGAAGTVHGALQAGARTTTFTASQGLLLMIPNMYKIAG
                     ELTSTVFHVSARTVATHALSIFGDHSDVMATRSTGFAMLASNSVQEAQDLALIAHAAT
                     LEARVPFVHFFDGFRTSHEVNKIEQLLEEDIRAMIDDELVHAHRARALTPDHPFVRGT
                     AQNPDVFFQARETINPYYQVCPDIVQKAMDRFASVVGRQYKLFDYVGAPDAERVIVIM
                     GSGAETAGETAKFLQENGESVGVLKVRLYRPFSVKYFLEALPPSVRAIAVLDRTKEPG
                     AAGEPLYQDVVTAINEGLAEGSVPYTQFAHRPYPRVIGGRYGLSSKEFTPAMVKGIFD
                     ELRKAEPKNHFTVGINDDVTFSSIEYDPLFTIDMPDTVRCVFWGLGADGTVGANHNSI
                     KIIGEETDNYAQGFFVYDSKKSGTVTVSHLRFGPRPIRAPYQISKANFVACHQFSFLE
                     RLDVTKYAAPGAVFLLNSIYGPDEVWDKLPVETQIDIIEQKLHFYVINAYDVARETGM
                     GGRINTIMQTCFFAISGILPRDQAIAEIKHAIERTYGKRGKAVVQANFAAVDKTLENL
                     FEVKVPEKVSSQLTRRPAVAAQAPEFVRNVVGKMIAGEGNSLPVSALPIDGTFPSATT
                     QWEKRNIALEIPVWEPDICIQCGKCTFVCPHAVIRAKVYEPSHLANAPEGWQADEARF
                     KEFPDKMYTLAVAPEDCTGCGLCIEICPVKDKTQVGRKAINLAPQPPIRDAECVKWDY
                     FLTIPEVDRTQMNLKVVKNSQLLQPLFEFSGACTGCGETPYVKLLSQLFGDHIVVANA
                     TGCSSIYGGNLPTTPWAINKEGRGPAWSNSLFEDNAEFGLGMRLTLDKHNEYARELLV
                     RLADVVGETLVDELLKADQTSESGIQAQRERVKLLKEKLNGVDDPRTRDLLSLCDVLV
                     RKSVWIVGGDGWAYDIGYGGLDHVLASGRNVNVLVLDTEVYSNTGGQASKATPRAAVA
                     RFAAQGKALPKKDLGMIAMAYGYIYVARVAMGASDQQTLNAFMEADAYDGPSLIIAFS
                     HCIEHGYDLRKGLDQQKLAVLAGHWPLYRFNPSFIAEGKSPLSIDSKGPSIPYEQYAY
                     NETRFKVLLHSNEQRAESLMKLAQKDARTRWELYQQMAQMHYDGSAEKNGKNGDNDS"
     gene            13971..14963
                     /locus_tag="AC16CB_07375"
     CDS             13971..14963
                     /locus_tag="AC16CB_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HIR3342326.1"
                     /translation="MIDLTTSYLGLSLKNPLVASASPLSKQLDSARQLEDSGVSAIVM
                     YSLFEEQIIQDSFKLDRDLDRGPYSSAEAFRYFPDYGMYSIGPESYLQHLRRVKEAVS
                     IPVIGSLNGVSSGGWVEYAQKIEQAGADALELNLYDLATDVSLTSAELEEQHIRLVQD
                     VSKEIKIPLAVKISPFYTALPNFASRLVEAGASGLVLFNRFYQPDFDLELLEVVPNLV
                     LSNSHELRLPLRWIAILYGRVKTDFALTSGVHSAEDLIKSVMAGASVAMTASALLKNG
                     VGRAREILDGMVAWMEEREYESVEQMKGSMSQQAVAEPGLFERANYMKVLSSYY"
BASE COUNT         3601 a         3793 c         4199 g         3583 t
ORIGIN      
        1 caccccccca aactccccca tcacctctcg aactgcgtaa cttatgaaac gattatattt
       61 acaccagcct tgcgaatatg ggcggcatag gatcgatagc ctttcgcaag gctgaattca
      121 cactgcagga gatattatat gccgatacat tttggcaccg atggctggcg cgccgtcatt
      181 tctgatacct ttacatatac caacttgcgc atggttgctc aagccatcgc cgacgcgatc
      241 agttcagctc agtggaacaa tgagactttg gataacggga atccggattc gcgggatatt
      301 atcgttggct tcgacacacg ctttctttct gatcgatacg caggggaggt ggcgcgcgta
      361 ctggcagcaa acggctacac agtccatctg gcccaagcag atgcccctac accggcaatc
      421 tcgtataacc ttaaacacct gggtgcgact gctgggatca tgatcacagc ctcgcacaac
      481 gctccgcgtt ataacggtgt caagctcaag gcggcttatg gaggttcggc gcttagcgaa
      541 cagtgccgcc gtgtggaagt ttatctgaat gataacgaag agcgtgcccg aggtccaaac
      601 ttgatggact acgaacaggc gcgccagttg gggctgatcc ggcgctttaa cccgctgcca
      661 gcctattacg atcacctgcg aaagttgatc gacttcgatc taattgccga gaacccgcag
      721 cgcgtggtag ttgattcgat gtatgggtcg gggaggggag tgttaaaggg tgtcctgcaa
      781 ggcacaggtt gcgaggtaca ggagattcgc ggtgaaatga atcccggatt tggcggcgtg
      841 cacccggagc ccatcgcaaa atacctgggg gcgctggctg gggcaatcag cacacacatg
      901 ggcaatttcg gcttagccac tgatggagac gctgatcgga ttggggcgat ggatgagcgt
      961 gggaattttg tcgaccctca taaaattatg gcgctgggat tgcgtcatct ggtagagagg
     1021 cgcggatggc gcggtcctgt agtgcgtacc gtgtctacta cgcggatgat tgaccggttg
     1081 gcaaaacaat acggcgtccc ggtatacgag acgccggtgg gttttaacca tatcgccaat
     1141 tatatgctga aggaggatgt ccttatcggg ggcgaggagt ctggagggat ctcttttcgg
     1201 ggtcacatcc cagagggtga tggtatcctg atgggcttgt tgattatcga gatggttgct
     1261 gcctcgggtg gtagcctgta tgaattggtt gaagaattac tccgtcaagc cgggccgatt
     1321 caataccagc gaaacgatct gcgcttgaaa caaccggtgc ccaaagatcg catgaaccag
     1381 cgcctcgttg agaacgcgcc tgcccaaatt gcaggtgaga atgtcttaga ggtctccaca
     1441 ctggatggcg tgaaatacat cctggccgac gactcctggt tgttaattcg tccttctggg
     1501 acagaacccg ttttgcgggt atatgccgaa gggcgcaccg cggagatggt gcggagcatg
     1561 attggctttg gcgagcaggt ggcagccagc tttacctaat aattatgcgg tgcgcagccg
     1621 ccgagccagg aagatcacga cgatcaacag aaccgccatg ccgagcaagc ccggcatgcc
     1681 tacttcatca gcgaaacccg tgctgggcaa ggcagtcgaa gtggcttgcg ttacggctgc
     1741 ggcttgcgtc agtagcgcgg ccacggttgc tgtacgcgga tctgaagagg gcacgggtgt
     1801 gttggtggcc attgccagca gcggagtggc tgtgcgcgcc agcgtcgctg tagggagcgg
     1861 cgtattggtt gatgtgggga cgatggcagc agcgatggct gtggatgtca atgcctgagc
     1921 cacctcgtta gcctgtgcgg tcaaagtagc gatttgggct tcctgttggg cccgttggcg
     1981 cggcaaaacg aggaacgcat aggctgcgat gcaggcgagc gccaaaacgg taatcccacc
     2041 taagatacca gctacaatca taaatgtccg gttgctggat tcctcggggg gtggtatttc
     2101 ctcgccagga aattcgatgt tgttagtatc cagttcttcc ataacactct cctcctaagc
     2161 tttatccggg atggtttatc ggatattcat ttatgctaat acctctaaat ttgccagcgg
     2221 caagagtgct ttttcgccgc tttccagttg gatctcggct acagcagctg agatgccatt
     2281 gggtaattca gacatacccg ggcggagttc cacaatcgta ccgatgctgc cttggtgagg
     2341 ggcgcggatc acgcgcacct gttgcccggc ggtaaatggt tcggtatctt gaggcagggg
     2401 tggatctaca ttggctggca agggtatgac gatctcgggt ctgttcccgg tgaatggatc
     2461 ccagggttga gcattgacgg ctacttcacg gcgttcattc gtcgatagca atttgaaagc
     2521 tgccgagttc atcgggtgct gcccgaagcc ttcgattacc aggattggga tacgaatacg
     2581 ctccgcaagc ggtattaagg cggcagccat gctggaaaag atcatggcgc gcataggcaa
     2641 gctcgcagcg gactttaata cttccggatt atcacagtaa ccgcctagaa tgatagaccc
     2701 gcgcaggcta acatccagtc gatcgggggt gaatacgtcg tctggattct tcatcagcac
     2761 atacatcaaa ccaaaatcaa gacgcccgtt tccccagaca ccttgaatta acgccccggt
     2821 tgtttctata gcggcgccca actcatccat caggtgcacc acagttcccg gcataccggc
     2881 tttgacctcg tatggaggtg attctagctc tagcaaaact tgcccgcctc ctgctacaat
     2941 aacgttgccg tttttagggg cacgcaccac tcgttttgcc attccaaccg gaccggctag
     3001 cacatctcct tgggaaacgc gcatccccgc cttacattga aggtactgat ctgctttttc
     3061 ttcgggcaaa cccaagccgc gagctatatt caggagcaaa tgttcgggat tcaggttgga
     3121 ttcagcgatc acatcagtgg tagataccat ctggccgcgg cgaacgagca ctttgccggg
     3181 gaccggcaag aggcggtcgc gacgaatgcg tgtcagaggg aggatatgga caacaggtgc
     3241 taacatagac tcaatgcctc atgaagaggg gtgcaacagt ccatcgacca gctttgttgc
     3301 aaggagagtg ccaacctaat catcagcagc ctaccatcca gcgccatttg gcgattaatt
     3361 cccgccggag ggcaggatca ttgggtaaat acaatgggcg tccgcgcgca tctataacaa
     3421 cgccgagcgc tccaccaacc acgcgcaatc ggccgccgcg gccggggccc cccatcccca
     3481 cgtcaaagcg gtgtaatggc tggatgtgca attgggcggt ctgcccagtg ggcagctgga
     3541 gcatctcgat agaaccttgt ttaacctcaa ggcttgtctc ggcaccgcct tcatagatca
     3601 tgcggatacg aaggatgggt gtgcctggac gagctacgcc aaccggggag atcactgagc
     3661 ctagggtgag gaaggtattg gattctaata cttgcacaac cattagtggg ttgattgctg
     3721 ccactgcgcc gagtgatggc gcaaggtggt tttggtccaa aaccagggtg gtgacaccgg
     3781 tgggttgcaa accatccagc agcattagca tggtttgagg tagattgggt gcacgagtga
     3841 gaacgctgcc cattgctaca atgggttcga accagggtga gagatctggt ccatagcgca
     3901 tcaccttttc aggaaaacca ttgcttgtct tttttatact gatctgcatg gcttgccgcg
     3961 ccagcgcttg ctctatggca agctcttctg gggtaacggg caaggaggca gggtagagtg
     4021 ctttgttgtg gatataatgg cgcacatcgt catctgacaa cccgatgtgg agccagcggg
     4081 taataccctg caacgggctg acgtttagga attccttcaa gccgtgtcct agtccaagct
     4141 gggggtatac gcgattgacc agattgcctt caaaggaagc ggctatgctg gtagcagaag
     4201 aacctacgtc tacacccagc acaccttttg tggaatcata aactttactc aagaagcgaa
     4261 tcacacggct aaaggctgtg gatgtaggca ataatccacc accagaccag ttgtccagct
     4321 ctgtaacacc gattatttgc tggctacgca cttgccgagc aatatgggca agatgcaact
     4381 gggcggattc caactgctca ttctccaagg tgggacgaac gttggtggcg aattgcagtg
     4441 gcgccagttt atcaaatgta gcctttatct cgtctcttaa cgactgattg ccggcaaaca
     4501 gtatctgtgg gcggtggcct tgaggtagca tatgtgcggc cagactaacg gcttctacaa
     4561 gcctgagaac agactgggat gcgccacctt cagtcccacc cgcgacgata accagatcgg
     4621 ggcgcaggcg gatgatcgca tccaggcgtt cttcgacagt gcggcggtca ttcaagctga
     4681 gggtgtccac aatttttgca taggatgtgt aaaccactcg cttggcactc tccaaggata
     4741 tgtcttcaag caaaccgatc acgactacat tgagcggggg tcccgcagat agtgtcgctg
     4801 cgacgatgtc tacgcctgca ccttcagatg tgctagggat gatgaggcgt tcatcggcgt
     4861 cgattaggat acgcccggtg acagtttgta gtcgatctaa ggcggtgcgc aggccttcgc
     4921 taatatcgtt atagggagcg ttgatcgtgg attgggcgga tccagaggcc aggaagcggt
     4981 aacgcccatc cacaacgtca aacaatgcag cccgtgttgt gatgctgcct atatcaatgg
     5041 caagtaccga atctgcgtca attagcgagg tgctcatgga aatctattag gatggaataa
     5101 gcgcgaagat gaaatcaata ataaaatcga cccgctcgat cagtgcggtc agtgtggcgg
     5161 caaagattcc ggcaaacagc gatcccaagg cgatggcaat gaaaatttgg ccgatccatg
     5221 caagaccttc cagccactta ggcctacccg caggttgatt tggatattgg cgggcaccga
     5281 agtgaaagta tgccaaagtt gccagagtgc cgatcagaac gacactgccc tttgccagtt
     5341 gtattacgta ctctcctcct tgttgaagat tttgttgatc gaacaagttt attgaagcga
     5401 gggattgagg gaaaatcgtc cccaggattg ccccgccaat tgctgtggct gcgcctaccc
     5461 caacaaggta agccaccgct ggattcccta gcggcgacag gcgaggcgag gctttgaaaa
     5521 gcaataagac tcccagaaca gatgggaaaa ggatatcgat tctatccgct tgtgtcccaa
     5581 ataccattgg gatgatcagt tgaggccaga tcacgttata ccaggctacc acggcagcat
     5641 atccagaggc gacaccgata aagatatgga tggcagtccg aaacatggcg ttgtcgccga
     5701 aggcgtagct gaaaaccatc agcgtcaaga agaagccaat gatagagccc agcatatcgg
     5761 ttaggctgag gtcagccagg ctcatcgttt ggccctccct tgacccggtt tcagggttgg
     5821 cagatacgca agcactatgt taaccaatcc cccgatcagg atcaaaaata cagcagttgg
     5881 gattccatag ctataggcat cccagtaacg gcgagcaatg cccgggcgtg gcatgctact
     5941 ttcataagcc acaccaccgc tcaacccgac caccaatcct ttggcttgct ttggatagcc
     6001 ttcgtagtat ggacgcacca gtggctcagc ttgggcgctg accaccaata gcaacggagt
     6061 atcctccaat gctggtccca cctgctcaat ccaattccta gccgtctctg ggctatccga
     6121 aatcaccact gtcagggcga aatcggacaa tgcttggata tttgaaagcc gcccattgat
     6181 ccagacggta ctttcgtctg cggtgctggc attgagagcg taaggcataa tctgctgggg
     6241 agattgcgca aagctgcgca atcctgagag cccgccgccg atatatccca ggttggcgta
     6301 ttgactggta tcgcgatatt ggtgtcccat ctggttattt acatagcgga ctaactgctc
     6361 tgcctgggct ggtccggtgg ttgaggtgga taccagggtc aggtatgcgc ctttgatcat
     6421 caggtgatcc agtaaagatg cagtcgtggc attcatctct ccagaatatg cgggctggta
     6481 atcgatagcc agtaaaacgt tgccgccatc tggaagggcg ttaataagca attgtgcatc
     6541 caggacctca ggcgataatt ctggggcggg tgcaccaaga tcgccggtat ataggggcca
     6601 aacgaccgat accaggagta tcaggaagag cgtgattctt actacagctt gggatgagat
     6661 ggttggggcg cgaggtatgg gagccgcgat gccttccgat ttcaccatct ctgctaaacg
     6721 ggaggcgaga gattgctgat tttcggaaac ctgcaacttg accgtgtaca cgggagattt
     6781 catctcacgt gcaatctctg gttcggcggg caaaatgcct tgtagacccg caagtggacc
     6841 gctactctct actatttggt cgagttcatc gctagctggt gcgcttggcg ctacagactc
     6901 gatgggtcgc atggattcaa gccaggtggg cagctccgca ggctcaagtt cgccagccgc
     6961 ttcttctgag ggttcagcct ctgtttgcgc ttcggccgca gggatttcct ttatccaatc
     7021 gggcaactct tcctggatta gatcggcgct ctcttcatcc aatgtgaagg gggccaccac
     7081 ctcaagctct gaatcctgat cttgctcatc ctctttggga ctgattccat ctttgatttc
     7141 gatatcctgc agccaatcag gttggtcttc gactggttct gcctcgaacg cgaaggaagt
     7201 tttctcttcg tcttcgactg gaagtgaagt catctcttct tgctcggcgg gtatttcgag
     7261 atcggctaac cagtcagcta tttctccttc ggcctcttcc tcatcgacag gctcagtaaa
     7321 gggaggttta tcagggggtg tttcagcagg caattctgta gcggattcaa gctcgatatc
     7381 ttcttccaaa aatggctctg taaaaggtgg ttggctgggc gtcattcggg atggaaattc
     7441 cgctaaagaa tcttcttcat cttcgggttc cgcgggtaat tcatcggatt tcaaccaatc
     7501 aggaagctca gggggcgtac tttcggtgcc tgattcagca tattctgcta gccagtcggg
     7561 aacctggctg tcttcttcat ctgggccggg ctgggcttct tcggcgtccg cttgctcaga
     7621 cgaatcggtc tcatcggcat cggggctcat tcgcagtttc gccaggaatt ccgcggattc
     7681 agcatccggc tcctctaagg ggagttcagg ttcgtctgag ctctggcgag cacggatgcg
     7741 ttggagccaa tcgggaatct cgtcttcagg catttcttct gagtaagttg attctgagtc
     7801 ctgctctgag ggtgtctcgg gctcggcttg gtttgccgcc cgcaagcctt ctaaccagtc
     7861 cggcttctct gagctatctt caggggctga agattcagcg gattcggttt cttggtctcc
     7921 tgggtcgact tcttcgctag accaatcggg gagctgatct tcgggctcta tctccccaag
     7981 ctcatcggta ccgctcccct gtcgcaatga ttgaagccaa tctggttgga gccactcggg
     8041 tagagcagga gtttgatccg gtggcttttt ttgagatggc gtctcattaa aggaagccga
     8101 ggaagatgtt atttcagcat cttcttctgg agcagaggaa atgttaagtg gcttcaggcg
     8161 cgcttggcaa tactggcagg tgtccagttc ttccggattt agtttgccac acattgggca
     8221 gcgcacttcg tccataatta accccgatat gggcggtcag cgcccatcag gatacgcaaa
     8281 cctgttgcaa cggtccctaa tgccacgcca agcagtatgc cacgagcacc agctactgcg
     8341 ggaatttggg cgatcaaggt gcggaatcca aacgcgccat gcaagccagg aataactaca
     8401 ccaaataatg gtgcgatacc caggagcgta atcaaacctg tgcctataaa tatgatcgag
     8461 aacaggttga gtcttcttct tatcaggcgc atagcggcgt aagtaagcac gacgactaat
     8521 atggccagaa ggctgctttc tactggcacc tggatattgt taaatatcca aagagaccac
     8581 tcagcagttg gtccgagata tcccactaca atcaaggtaa tgagcaagga tatgatgagg
     8641 accgcactat agaaactgcc attctgttca gaggaggcct tggtccagtg aacagaggcg
     8701 aggtttgcta tcccaaccag taaggcaaca gccgccaaaa tgacagccca ttgcaggaac
     8761 atgttgtgca gatcgctcag ccaagggatc tcgataaaat aacctgcgag aacaatgagg
     8821 ccgaagagta ttgcaatggc tgccgagaag ggcactttga ttttcatagg atctggagga
     8881 atttcaggat cgctcccagc aagatagcca gaattagtac ccagcggagc acatcctggg
     8941 cgcgcaggct ggcgttatgg aataagcccg ctttaagata ggcgcctgcg gcatagagtt
     9001 cttcgccgat gagtggttct tgggcagcag catacagaac tgcctgggca gacacctcgt
     9061 cactgcccgc cagagttgtc gcgccaacgc gctcggatgc gtctgtgatc aatgccacct
     9121 cgctgccaaa gtgaccacta aggattgtgg tggaaacgtg ctcatcatag atggcgggca
     9181 atgttccagc ggcatacgcg aaaggcgtca acccgctaat ccgccctaaa gaagcgtcat
     9241 attgacctcc ctggcccact gccctgaagg agttatacag cgtatcctgt gagagtatgc
     9301 ctagcgtgcc ctccccgctg gtagcaatgg gtgggcgatc gctaatggaa gccgtgcggg
     9361 cgatgcgatc aagtatgctt aagccaatca atgcagatcc gctttgcagc ccagagagat
     9421 ttccgcgtcc tagggtgagg tgaagtcgtg tgcccgcttc aacagccaac ccgaccgccc
     9481 gccacagctt ttgaaaggcg ggaatctccc gcagagcagc gggggggcgt cttcgaccaa
     9541 agaaggcgaa gatgaccagg ataatgaaaa ataatataac gaaaaacaaa cccatttggc
     9601 caataccaaa cagattaggt ggaatcactg aggtgcttcc tcctgcagca atgtaacaaa
     9661 cgctttggtt tgatcaggtt gttctagaat ttcggtaaga gcggaaaggt ggtccgatgg
     9721 aaagatcgag gagatgaacg gttgaactaa atatactgct tgagcaccca gcacggcgag
     9781 cgggccaagt gcttcaagga aagtagcagc ccagacccct accccccacc tgtgtgcctt
     9841 tttcgcccaa acccgccaga tatgctggtt ggaatacatg tgcaaagtat agcacaaatg
     9901 tgtggggttg gtaaaggtca aatatgattg cggattattc aatatagtcg aatttaattg
     9961 gaactttcaa aattaaaagc ataggactta cgcagttgaa gagggaagag ggggaggttg
    10021 ggcaggtcat gacccgccca aactccccca tcacctctcg aactgcgtaa cttatgaagc
    10081 aattacacaa tcaaggattt aattttctga tcacatacac tctacttaat aacacctaaa
    10141 attgtctgaa atatgacagg tctcagggtt ttacatgcct aaaataacta tgtgaaaatt
    10201 cgaagcggga ctaaactgtt aaattgaaac taacaactat tctcattgtc gacctaccca
    10261 acctgggcag gcagatttgg agcgaattca tgaaacgacc tatggttact attgacggta
    10321 atgaagccac agcatttgta gcacatcgag tgagtgaggt tatcgcgatc taccctatta
    10381 cgccctcttc atcgatgggt gagcacgccg acaagtggtc ggcgattaat cacaaaaata
    10441 tctggggcac cgtgcctctg gttgtagaaa tgcaatctga gggtggtgca gctggaacag
    10501 tccacggtgc gttgcaagct ggtgcgcgga ctactacgtt caccgcatct caggggctgc
    10561 ttttgatgat cccgaatatg tataagatcg ctggcgaatt gacgtctacg gttttccacg
    10621 tctcggcgcg taccgttgca acccacgccc tatcgatctt cggagatcat tccgatgtaa
    10681 tggcaacacg gagcactggc ttcgctatgc ttgcatccaa ttctgtgcag gaggcacagg
    10741 atctagccct gattgctcat gcagctactc tggaagcgcg cgtgcctttc gtgcatttct
    10801 tcgatggctt ccgcacatcc cacgaggtca ataaaatcga acaactgctc gaggaagata
    10861 tccgggcgat gattgacgat gagctagtgc acgctcaccg ggcacgcgct ctcactcctg
    10921 accatccctt cgtgcgaggc acagcccaaa atccagatgt gttcttccag gcacgcgaga
    10981 cgataaatcc ttattaccag gtttgccctg atatcgttca gaaagcgatg gaccgtttcg
    11041 ccagtgtggt tgggcgacaa tataaactct ttgattacgt cggtgcacct gacgctgagc
    11101 gggtaatcgt catcatgggc tccggcgcag agaccgcagg cgagacagcc aagttcctgc
    11161 aagaaaatgg tgagagtgtt ggcgtgctca aagtacgcct ataccgtccg ttctcggtga
    11221 agtatttctt ggaggccttg ccgccttcgg tgcgcgccat cgcggtttta gatcgaacca
    11281 aagagccagg cgcagctgga gagccgctct accaagacgt ggtcaccgcc atcaacgaag
    11341 gcttggcaga aggcagcgtg ccgtataccc agtttgcgca tcgaccgtac ccgcgtgtga
    11401 tcggcggtcg ttatggtctt tcatcgaaag agtttacccc tgccatggtc aagggcatat
    11461 ttgatgagct ccgcaaagca gaaccaaaga atcacttcac cgtgggcatt aatgacgatg
    11521 tgacttttag cagtattgaa tacgaccctt tatttaccat tgatatgccc gatactgtgc
    11581 gctgtgtgtt ctgggggctt ggcgcggatg ggacggtggg cgccaaccat aactcgatca
    11641 aaatcattgg cgaggagacc gacaactatg cccagggctt ctttgtgtac gactcaaaga
    11701 aatctggcac ggtgaccgtc tcgcatttgc gttttggccc gcgcccgatc cgtgctccgt
    11761 accagattag caaggcgaac ttcgtcgcat gtcaccagtt ttccttcttg gagcgtttag
    11821 atgtaaccaa gtacgccgct ccaggggcag tctttctcct caacagcatc tatggtcccg
    11881 atgaagtttg ggataaattg cccgtagaaa cgcaaataga catcattgaa cagaagctgc
    11941 acttttatgt gattaacgct tacgatgtcg cccgcgagac gggcatgggt ggtcgcatca
    12001 ataccattat gcaaacctgc ttcttcgcca tcagcggaat attgccacgc gatcaggcga
    12061 tcgccgagat caagcacgct attgagcgga cctatggcaa gcgcggtaaa gccgtggtac
    12121 aagccaactt cgcagctgtc gacaaaacct tggagaacct gttcgaagtg aaagtccctg
    12181 agaaagtgtc tagccaactc actcgtcgtc cagcggtggc tgcccaggca ccagagttcg
    12241 tgcgcaatgt agtcggaaag atgattgctg gcgagggtaa tagcctgcct gtaagcgctc
    12301 tgccaattga tggcacattc ccgtcggcta ccacgcagtg ggagaagaga aatatcgccc
    12361 tggagatccc ggtgtgggaa ccggatatat gcattcagtg cggcaagtgc acctttgtct
    12421 gcccgcatgc tgttatccgc gccaaagtat atgagccttc tcacttggcg aatgcgccgg
    12481 agggatggca agcagacgaa gcacgtttca aagaattccc ggataagatg tataccttag
    12541 cggtggcgcc cgaggattgc acgggatgtg gtttgtgcat cgaaatttgc ccggtgaaag
    12601 ataagaccca ggtggggagg aaggcgatta atttggcgcc gcagccgccg atacgcgatg
    12661 ctgagtgtgt caagtgggat tatttcctga ccatacctga agtagaccgc acccaaatga
    12721 atctcaaggt agtgaaaaat tcacagttac tgcaaccttt gtttgagttt agcggtgcct
    12781 gtactggatg tggtgagaca ccctatgtaa agctactctc ccagctcttt ggagaccata
    12841 tcgtggtagc gaacgcaacg ggttgctctt cgatctacgg cggaaatttg cccaccacac
    12901 cctgggctat aaacaaggaa ggccgcggtc cagcctggtc gaactctctt ttcgaggaca
    12961 acgctgagtt cggtttgggt atgcgcctga cgctggataa acacaacgag tatgctcgcg
    13021 agctgctggt tcggctggca gacgtggtgg gcgagacact ggttgacgag ctgctcaaag
    13081 cggaccagac tagtgagagc ggtatccaag cacaaagaga gcgtgtgaaa ctgctcaagg
    13141 aaaaacttaa cggtgtggac gacccacgca cgcgtgatct gctcagccta tgcgatgtcc
    13201 tggtgcggaa gagtgtatgg atcgtcggag gtgatggctg ggcgtatgac attggatatg
    13261 gcggtttgga tcatgtgctc gcctccgggc gaaatgtgaa tgtgttggta ctggatactg
    13321 aggtgtactc gaacacaggt ggtcaggcat ctaaagctac cccacgcgct gcagttgccc
    13381 gcttcgccgc tcaaggcaaa gcccttccaa agaaggacct gggcatgatt gctatggcgt
    13441 atggatatat ctatgtggct cgcgtcgcaa tgggagccag cgaccagcaa acgctcaacg
    13501 ctttcatgga ggcagatgcc tacgatgggc catccttgat tatcgccttt agccattgca
    13561 ttgagcatgg ttatgacttg cgcaaaggac tggatcaaca aaaacttgcc gttctggctg
    13621 gtcactggcc actgtaccgt ttcaacccct cctttattgc agaagggaaa agcccgctca
    13681 gtattgactc gaaaggtccc agtatcccat acgagcagta cgcgtataac gagacacgct
    13741 tcaaggtgct gttgcacagc aacgagcaga gggctgaatc cttgatgaag ctggctcaaa
    13801 aagatgctag gacgcgctgg gagctgtacc agcagatggc ccaaatgcat tacgatggct
    13861 ctgcagagaa gaatggaaag aacggagata acgattccta agtagggagg tgggattaga
    13921 ataggagcta catcctatcc ctaattcccc tttataatga ggagttatat atgatagatc
    13981 tgaccacgtc ttacctgggt ttatccctaa aaaatccctt ggtggcttct gcctcccctc
    14041 tttcgaagca gctggattca gcccgccagc tggaagattc tggtgtatct gcgatcgtga
    14101 tgtattcatt gttcgaggag caaatcatcc aggatagctt taagttggat cgtgacctgg
    14161 atcgggggcc atattcatct gcggaggcgt ttcgctattt tccagattac ggcatgtaca
    14221 gtatcggtcc tgagagttat ctgcagcatt tgcgccgggt taaggaagcc gtgagcatcc
    14281 ctgtcatcgg aagcctgaat ggggtttctt ctggcggttg ggtggaatac gctcaaaaaa
    14341 tcgagcaagc cggagctgat gctctggagt tgaacctgta cgatttagca accgacgttt
    14401 ccttaaccag cgctgagctg gaagagcaac acatccgttt agtacaggat gtgagcaagg
    14461 aaataaaaat ccccctggcg gtcaaaatca gcccctttta cactgctttg ccgaacttcg
    14521 ccagccggct ggtggaggct ggggcaagcg ggttggtgtt attcaaccgt ttctaccagc
    14581 cagattttga cctggagttg ttagaggtgg tgccaaacct ggtgttgagc aactcgcatg
    14641 aattgcgctt gcccttacgg tggattgcta tcctttacgg acgggttaag actgattttg
    14701 ccctcaccag tggagtgcat agtgctgagg atttgatcaa gtcggttatg gctggtgcca
    14761 gtgtggcgat gaccgcatcg gcgttactca agaacggtgt gggtcgtgcc agggaaatcc
    14821 tggacggcat ggttgcctgg atggaggagc gtgaatacga gtcggtcgag cagatgaaag
    14881 gtagcatgag ccaacaggca gttgctgaac caggcctttt tgagcgggcg aactatatga
    14941 aggtgctcag ctcatattat taatatcctg ctggctcaac cctgatttta tttgaattag
    15001 agggcaggtg attatcatta atcccccggt acaaaagggt tagcacaaag tagccgaatt
    15061 gggtttctat acttcccagt tcggctcatt ttttcgctct attggcttat actacaggaa
    15121 tatggctgct caccaagttc aactttttgt cacttgtatt attgatacgc tgtatc
//
LOCUS       DBNGSX010000241         4020 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_504635, whole genome shotgun sequence.
ACCESSION   DBNGSX010000241 DBNGSX010000000
VERSION     DBNGSX010000241.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_504635"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="AC16CB_07380"
     CDS             complement(<1..239)
                     /locus_tag="AC16CB_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HIR3342327.1"
                     /translation="MEIHIGSHTIGLKHPTYFIADIAANHDGDLERAKLLIRLAKETG
                     ANAAKFQNFRAPQIVSDYGFRSMGGQVSHQANWKKS"
     gene            complement(330..1079)
                     /locus_tag="AC16CB_07385"
     CDS             complement(330..1079)
                     /locus_tag="AC16CB_07385"
                     /inference="COORDINATES: protein motif:HMM:NF015146.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HIR3342328.1"
                     /translation="MNLLLEIARCPNVITCVKKPSEQHPCSQVIASQKATSLDSFHVP
                     EPWNGLIEQAPLLFISSNPSFNRKEEFPLWSWDESLIEDYFTHRFGGGKKLWVKDGVQ
                     GLLRKGGYADVTPFWEAVMHRATELFERPVKPGVDYALTEVVHCKSRRQKGVDEALDE
                     CSQRYLKRVLEISGANLIVVLGATARKVVKDLFSISPRTHLSDPIVIKNMARIFVFLP
                     HPNAYLPNTFSENFSAEELKQIQAHLFKASQ"
     gene            complement(1160..2092)
                     /locus_tag="AC16CB_07390"
     CDS             complement(1160..2092)
                     /locus_tag="AC16CB_07390"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009226 - nucleotide-sugar biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3342329.1"
                     /translation="MRILVTGASGLLGLNLALSAAGQAGEGHLVIGTVKDHALKTDAF
                     RTIQTDLLIPGAVERLLDQTQPDWVIHCAAMANVDACEADPEQAKQMNSELPGKLAAY
                     VARGGARLVHISTDAVFDGQRGDYLEEDVPNPLSIYARTKLEGELSVAQANPEAIIAR
                     VNLFGWSISGARSLGEFFFYNLSAGKRVRGFTDVFFCPILANEIAPILIRMLEKKLSG
                     LYHVVSRECTTKYEFGVRIARRFGLDESLITPFSVAEAGLKAVRSPKLTLRTDKLAAT
                     LGEPPPDLSTGIEGFYTLYQQGYPQILKGIGGRG"
     gene            complement(2205..3218)
                     /gene="pseB"
                     /locus_tag="AC16CB_07395"
     CDS             complement(2205..3218)
                     /gene="pseB"
                     /locus_tag="AC16CB_07395"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HIR3342330.1"
                     /translation="MDWSDKVVLVTGGTGSFGKKFIRIMLDEYHPAKLIVFSRDELKQ
                     HEMRVSGFDHPSLRYFIGDVRDVERLRRAMHGVDIVIHAAALKQVPACEYNPLEAIKT
                     NILGSSNVIEAALDMTVKKVMALSTDKAVNPVNLYGTTKLAAEKLFVQSNAYAAGTST
                     RFSCVRYGNVVGSRGSVVPIFVQQRKNGKLTITDERMTRFWLTLEQGVRFVIRCTEQM
                     LGGEVFVPKIPSMKIIDLAQAIAPDTEMEIIGIRPGEKLHEVLIHEDEARSAVELDDM
                     FVVQPATELWFGHEWQKLGKSLPDGYRYASNTNPEWLDIQQIQQMVAPFERDEGQIMI
                     DEG"
     gene            complement(3317..>4020)
                     /locus_tag="AC16CB_07400"
     CDS             complement(3317..>4020)
                     /locus_tag="AC16CB_07400"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3342331.1"
                     /translation="LLDAGSGPIQYPAYLEYSKGYQRRVCADISIIALKEARQRIGEH
                     GHYVVCDIANLPFKAEAFEGIVSLHTIHHLPETEHVHAYSELYRLLSPGCAATVVNGW
                     PGSRLMRWAEPLIRLNRRIRSILRRLLKRTQPTAHRARHSARDTSQVTADTPKGTYTS
                     RHDAAWIRSEVARHMPVQIWVWRSVSVRFLRNFIYPQLGGRLWLRLLYWLEERFPHWL
                     GENGQYPLIVIRKVE"
BASE COUNT          879 a         1082 c         1094 g          965 t
ORIGIN      
        1 gattttttcc agttcgcctg gtgagagacc tgcccgccca tagagcggaa accgtagtcg
       61 gagacaatct ggggagcgcg gaagttctgg aacttggccg cgtttgctcc ggtctccttt
      121 gccaggcgga tgagcagctt ggcgcgttcc aggtcgccgt catggttggc ggcgatatca
      181 gcgatgaaat aggtggggtg cttcaggcca atagtgtggg aaccaatgtg gatttccata
      241 gagactccga gattctagag tagagaggat gatcgtataa gccagtctat tatgacaaac
      301 aggaagacaa tggcagcgtg gcaatctcct cattggctgg ctttgaataa atgtgcttgt
      361 atttgtttca attcttcggc agagaaattt tccgaaaaag tattgggtag gtatgcgttt
      421 gggtggggaa gaaagacaaa aatacgggcc atattcttaa tcactatcgg atcagataag
      481 tgtgtgcggg gagaaatgga aaacaagtct tttaccactt ttctcgctgt tgcgccgagg
      541 acgacgatga ggtttgcacc agatatctcg agaactcttt tcaaatagcg ctggctgcat
      601 tcatcaagcg cctcatccac tcctttttgt cggcgcgact tgcagtgtac gacctctgtc
      661 agtgcgtaat caaccccggg ttttacaggt cgttcaaaga gttcagtagc cctatgcatt
      721 accgcttccc aaaaaggagt gacatctgca tagccgcctt tacgtaataa cccttgcacg
      781 ccatctttta cccataactt ctttccgcct ccgaaacgat gggtaaaata atcctcgatt
      841 aagctttcat cccacgacca aaggggaaat tcctctttcc tgttgaatga aggattggaa
      901 ctaataaaca gcaaaggggc ttgctcaatc aagccattcc agggttcggg aacatgaaaa
      961 ctatctaggc tggtagcctt ttgactcgca ataacttggg agcaagggtg ttgctccgat
     1021 ggtttcttga cacaagtaat aacatttgga caccgagcga tttctagtaa cagattcatc
     1081 tttcacctca aaatatgcac aagaaatccc atgaagcaat actttaattg attgttcagg
     1141 atgtaagggg agtttttccc tatccccttc cccctatccc cttaagaatc tgtggataac
     1201 cctgttgata cagtgtgtaa aagccctcga ttccggtgga taaatccggt ggcggctcac
     1261 ccagggtagc agccagctta tcggtgcgca gggtaagctt gggagagcgc acagccttca
     1321 agccggcctc tgccaccgaa aagggggtaa ttagactttc gtccaggccg aagcggcgtg
     1381 cgatgcgcac gccgaattca tatttggtgg tgcattcccg gctgactaca tggtacagcc
     1441 cgctgagctt cttctccaac atgcggatca agattggggc aatctcgttt gccaggatgg
     1501 ggcagaaaaa cacgtcggtg aagccgcgca cgcgcttgcc tgcactgagg ttatagaaga
     1561 agaactcgcc caggctgcgg gcaccgctga tgctccagcc gaacaggttg acgcgggcga
     1621 tgatcgcctc gggatttgcc tgcgctacgc tgagctcacc ctccagcttg gtgcgggcat
     1681 agatgctcaa tggattgggg acatcttctt caaggtaatc gccgcgctgc ccgtcaaaga
     1741 cggcatcggt agagatatgc accaggcgcg ctccgcccct ggcgacatag gctgccagtt
     1801 ttccagggag ctcactattc atctgttttg cctgctcagg atcagcttcg caggcgtcta
     1861 cgtttgccat cgcagcacag tggatcaccc aatcgggttg agtctggtcg agcaggcgct
     1921 ccactgcgcc ggggatgagc aaatcggtct ggatggtacg gaaggcgtcg gtcttgagag
     1981 cgtgatcctt gaccgtgccg atgacgaggt ggccttctcc ggcctgcccc gccgcgctca
     2041 gagccagatt cagccctagc aggccgctgg ctccggtgac cagaatgcgc ataattcacc
     2101 tatataagca gcaattagct attgttctgc gtctttcgtc ctccgtctac tcattatctg
     2161 aggtcgacca atattcatca gccccagatt taatgcttca gctttcatcc ttcgtcaatc
     2221 attatttgcc cttcgtctct ttcgaagggc gcaaccatct gttggatctg ctggatgtca
     2281 agccactctg gattagtgtt gctggcgtaa cgatagccat ccggcaggga cttgccaagt
     2341 ttctgccact cgtgaccgaa ccacagctca gtagcaggct gcacaacaaa catatcgtct
     2401 agctccaccg cagagcgggc ttcatcctcg tgaatgagca cttcgtggag cttctcaccc
     2461 gggcggatgc cgatgatctc catctcggtg tctggagcga tggcctgggc cagatcgata
     2521 atcttcatgc tgggtatttt gggcacaaag acttcgcccc caagcatttg ctcggtgcaa
     2581 cggatgacaa agcgcacgcc ctgttccagg gtgagccaga agcgggtcat gcgttcatcg
     2641 gtgatggtca acttgccatt cttacgctgc tgcacaaaga tcgggacgac gcttccccgg
     2701 ctgcccacca cgttgccata gcgcacgcag ctgaaacggg tgctggtgcc ggcggcgtag
     2761 gcgttgctct gtacaaagag cttctctgca gccagcttgg tagtgccgta caggttaacc
     2821 ggattgacgg ccttatctgt gctcagtgcc atgaccttct ttacagtcat atccagagcc
     2881 gcctcgatga cgttgctgct gcccaggatg ttggtcttga tcgcctctag cgggttgtac
     2941 tcgcaggctg gcacctgctt gagagccgca gcgtggatga cgatgtccac cccatgcatg
     3001 gcgcggcgca agcgctctac gtcgcgcaca tcgccgatga aatagcgcag gctggggtga
     3061 tcaaagccgc tcacgcgcat ctcgtgttgc ttgagctcgt cgcggctgaa gacgatcagc
     3121 ttggcgggat ggtactcgtc cagcatgatc ctgatgaact ttttaccaaa cgatccggtt
     3181 ccgccggtta ccagaacaac tttatcgctc caatccataa ttcctcgctt atgctaactt
     3241 accgtagaaa cactgagtat gcagatgttt ctcctgcggt atctcagtat atattgcatc
     3301 tctacggcga aattcttcat tctacctttc ttatgacaat caacggatac tgcccgtttt
     3361 cgccaagcca gtgcgggaaa cgctcctcca gccagtaaag cagcctcagc cacaggcgtc
     3421 cgcccaactg ggggtagata aaattgcgca ggaagcgcac gctcacactg cgccagaccc
     3481 agatttgaac cggcatgtgc ctggcgactt cgctcctgat ccaggctgcg tcatgacggc
     3541 tggtatatgt tcctttgggg gtgtcagctg tcacttgcga agtgtcacgt gcggagtgcc
     3601 gagcgcgatg tgcggtgggc tgagtccgtt ttagcaggcg gcgaaggatg ctccttatcc
     3661 ggcggtttag gcggatgagc ggctcagccc accgcatcag ccggctgcca ggccagccat
     3721 tgaccaccgt tgccgcgcac ccgggggaca ggaggcggta taactccgaa taggcgtgca
     3781 catgttcagt ttctggtagg tgatggatcg tgtgcagcga gacgatgcct tcgaatgcct
     3841 ccgccttaaa gggcaagttg gctatatcgc aaaccacgta gtgcccgtgc tccccaatgc
     3901 gctgccgggc ttccttcagg gcgatgattg agatatcggc gcagacacgg cgctggtagc
     3961 ctttggaata ttccaggtac gcagggtact ggatcggccc ggagccggcg tccagcagaa
//
LOCUS       DBNGSX010000242        11887 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_88963, whole genome shotgun sequence.
ACCESSION   DBNGSX010000242 DBNGSX010000000
VERSION     DBNGSX010000242.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11887)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11887)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11887
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_88963"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="AC16CB_07405"
     CDS             complement(<1..391)
                     /locus_tag="AC16CB_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR3342332.1"
                     /translation="MELSVLPELLAVCQLSPDEELPPWAGQSDLLAVIRDGDELTIVC
                     AQRFVPPGVQAEQGWRALKISGPLDFSLVGVLAELAATLAQAGVSIFALSTYSTDYIL
                     VKDEVLENAIRALRSSGHLISLVSRLDQ"
     gene            complement(391..1623)
                     /locus_tag="AC16CB_07410"
     CDS             complement(391..1623)
                     /locus_tag="AC16CB_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor synthesis domain-containing
                     protein"
                     /protein_id="HIR3342333.1"
                     /translation="MPEFLELLPPPEALAQLLAHLPIHPEPEEIDTEQALGRVTVEPV
                     IAPHPLPSFPRTTVDGYAVRAGDTFGASDSLPAYLQLIGEIPMGGVPGFSLAAAQCAL
                     IHTGGMLPEGADAVVMVEHTQSARPGEVEVLRAVAAGENVLKVGEDVLPGDEVIPAGV
                     RIRPAEIGGLMALGMIKTQVSRRPRVGIISSGDEVVSPEKTLGPGQVRDVNAYTLSSL
                     VEAAGGQPVRYGIVSDQEKVMREVASKALAECDVVVITAGSSASTRDLTSQVVNSLGK
                     PGVLVHGVNVRPGKPTILAVCNNKAVIGLPGNPVSALVIAWLFVIPVVEALLGLAYRQ
                     PRPSASARLTLNLSSQAGREDWIPVRLVPGAGGYQADPVFGKSNLIFSLARADGLVRI
                     PADATGLEAGEQVEVYLF"
     gene            complement(1676..2596)
                     /locus_tag="AC16CB_07415"
     CDS             complement(1676..2596)
                     /locus_tag="AC16CB_07415"
                     /inference="COORDINATES: protein motif:HMM:NF013226.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HIR3342334.1"
                     /translation="MADSRSQNPFVLFFRISRPLFLLGAVLIYALGAGVADYLGTSID
                     WSVYLVGQLWVIALHLSGVFLYEYYTAVSEASNPYRDPLSGGDGKIGPIRTPVTMLVT
                     AAVSLTVVATLSVWMLRELRLSPVAWLVMGLACLGAVFYSVPPVRLAYSGYGELVIAI
                     LYANLLPAFAFVLQEGDLHRLLAMSTFPLTALMLALIMAFELPDYAIDIKQDRRNLMV
                     RLGWQRGMGLHNGMIFVAFLLIGLALTFGMPLFIGLPALLTLPLGLLQIWQMRRIAEG
                     VKPNWMALKLTAVVLFATTTYLLTFAFWTR"
     gene            complement(2589..3086)
                     /locus_tag="AC16CB_07420"
     CDS             complement(2589..3086)
                     /locus_tag="AC16CB_07420"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3342335.1"
                     /translation="MTDSPTRIAERMQVEGKRTLEFFRELRPDQWRAIMYTEGSVWTV
                     REVLAHFVSAESGMTRLVENILAGGLGSPDDFDLNAYNERKVASLKEASTDELLGQFA
                     FLREKSAALAANMNPDDLTKTGRHPWLGMASIADILKMMYRHNQIHLREIRRMMNSSE
                     PEQDG"
     gene            complement(3227..3574)
                     /locus_tag="AC16CB_07425"
     CDS             complement(3227..3574)
                     /locus_tag="AC16CB_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3342336.1"
                     /translation="MPQRRTSPSILPFVLVAVGLVLILSAGVWYYNMTLSPPELVQPS
                     SPVEEENYPDIARVSLADAKAAYDTGSAVFVDVRSADSYAQSHIPGALSIPLAELPNR
                     ISELNPSDWIITY"
     gene            complement(3725..4687)
                     /locus_tag="AC16CB_07430"
     CDS             complement(3725..4687)
                     /locus_tag="AC16CB_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013761.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HIR3342337.1"
                     /translation="MLRSFLIYLSQAAWARKIVTSWSIAWKAASRFIAGETLNDAINV
                     VKKLNEKGINATLDHLGEHTTNPDEARRATQDILRILDAIQQTGVRSYVSIKLTQIGL
                     ALDESLCEENLMTILERARQVGTYIRIDMEDSPWVDKTLALYRKAHLEHGFEHLGVVI
                     QSYLYRSEQDIHSLCEECTGVRLCKGAYKEPPDVAFPRKKDVDANYDLLARLMMDSAL
                     QRGCPQLSDDGRIPPPPVFATHDEQRVINALNYAQQIGLPKKAMEFQMLHGIRRDLQQ
                     KLAADGYPVRIYIPYGTEWYPYFIRRLAERPANLWFFVSNFFRR"
     gene            5005..6420
                     /locus_tag="AC16CB_07435"
     CDS             5005..6420
                     /locus_tag="AC16CB_07435"
                     /inference="COORDINATES: protein motif:HMM:NF016827.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HIR3342338.1"
                     /translation="MLQQSQAPITRPITTAHLATTMSLMSLTAIELHERIEAELASNP
                     ALELGEKHHCTSCGRPLVEKGTCLVCSRLSPGLGEEPIVFISPREDFYLPARQSTPNG
                     IPGDEITAETEDLPTYVLRQIAPELAPEDRLLAAHLLTSLDEDGLLRVPLIEFARYHH
                     RPPSHIEQLLKLIQRADPIGVGSSSPEEAMLVQLEVLGETQSIPPMTDQVIRQGIGLL
                     SRRHFTELGKLLGISASQVKQIARFISGNLNPYPARSHWGNVHQGTEPAQDVYHFPDI
                     LISRLNDNPDSSLIVEVISPLAGRLRLNLLFRQSIRLAPEDKVEEWQNDLEQASLLIK
                     CLQQRNHTIVRLMQRIAAIQRHFILHGDAYLEPLTRASLAEELQVHESTISRAVAGKA
                     VQLPNGKIVPLSKMFDRSLHIRTELRQIIAHECQPLTDTELAALLKKKGYRVARRTVA
                     KYRAMEGILPAHLRQPLQTAG"
     gene            6428..8398
                     /locus_tag="AC16CB_07440"
     CDS             6428..8398
                     /locus_tag="AC16CB_07440"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR3342339.1"
                     /translation="MNTPLKLNMLPGLRPTPGLAEIESMMELFKSACLLVESRNRRIL
                     LANSQATELTAYTRGELSGMAFSKLIDQPNKHPFWESPSGQPFPTALTLLRRNGAKVS
                     VQATRHELAAQSKWAIIQLEESHVIEQRQSQQRRQSGMIQSMVTISQAIGLPDLSEAI
                     TMILEASHEITGCDNIGVYLQEMATKGQGFEMVRYAQHGADNILPERLPAQDLVHLRA
                     PNYWIPGKRSASSLHRAARSAGLAFLASAPLGQDKAPVGFIAIAGGAPIAQNEILPPL
                     EILANIIDVLIQIHTRTTNLEKSLGDNIRARALEKTSMDAIEDGIIVLTPTLNVVRLN
                     PAAETILGYTNREARNRPVEDILIGTGALTTSLELALQGVPTIKQENPHLYRRSGTPF
                     LAQVSIIPAHVEDHLEGIVVLIRDLSEQEQIQVQAEQLKQRAVLGEVSSIFAHEVRNP
                     INNISTGLQVMAYNLSPDDPHQEVISRLQRDCERLDELMKSVLTIGRPAEYKLGPTDL
                     SMLATRLVERFKPRMVNANIQPFLQIEPSLPLVNGNLLALDQVFINLINNAIEAMSEK
                     GGTLALRLQTATDGDGKRFVEVNVADNGPGIPKEVQERLFQPFFTTKKDGNGLGLVIT
                     RRILNAHKGTITLTSYPGATIFHVQIPVLESP"
     gene            8395..9570
                     /locus_tag="AC16CB_07445"
     CDS             8395..9570
                     /locus_tag="AC16CB_07445"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3342340.1"
                     /translation="MSLTVLIVDDEENARYNISSFLGGRGYEVLGVSTLSEARETLRR
                     GNADIILLDVTLPDGFGTDLLEETAHMPNRPPIIMITAYGNIEMAVNAMKNGAHDFLP
                     KPIQLAELEKSIQRGGELVALRRELAHLRSVQHNQVDFVVGQTPVMRNLLNQAQRAAA
                     ASVSVLITGETGTGKEVLARAIHGMGPRSSKPFIGENFAAIQPTVLESELFGYEPGAF
                     TGAEKRKIGLMEVAEGGILFLDEISSLSVDMQAKLLRALEERAFRRVGGNNQIRVDVQ
                     FLAASNRHLPTLITEGKFREDLYYRLKVVDLHLPPLRERKEDIPELVGLFIRQLNPRM
                     GLNITDVTPRALQALMSHNWPGNIRELRNIIERAMLFCDDAAIDLPHLPAELSNGKE"
     gene            9681..10079
                     /locus_tag="AC16CB_07450"
     CDS             9681..10079
                     /locus_tag="AC16CB_07450"
                     /inference="COORDINATES: protein motif:HMM:NF024247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3342341.1"
                     /translation="MTYIITSLCLRDAGCVEVCPVECIVPGKPQEEWPWYYIDPDTCI
                     DCGACVPECPYEAIFPEDEVPTDYTAKGGEYVSKVGLTGHYEGTNHHDEAVVLDSVRQ
                     LEAGEVVDLTADNQANYDYFTSGPGYSALE"
     gene            complement(10155..11024)
                     /locus_tag="AC16CB_07455"
     CDS             complement(10155..11024)
                     /locus_tag="AC16CB_07455"
                     /inference="COORDINATES: protein motif:HMM:NF028425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HIR3342342.1"
                     /translation="MLPKSMLIALSALALVTLACGFTIDVPVKEVNIGPLVTEDVSVS
                     LPEQQGDVTRVEFSFGAGEFTLAPGAQDALVNGTASYNVRDLKPQVSVDKNEVLITTG
                     DFEIDGIPRFGDEYKNVWDLRLGDVPMDLRINAGAYKGEFELGGLKIESLRVSDGAAD
                     AQLSFSVPNKSEMDTFRYTTGASRVEMTGLANANFDSMVFKGGAGDYTLDFSGELKRD
                     ATVTIDSGFSSVKLIVPEGVLTRVFVDSGLANVDIGGEWEKSGNEYTLSGEGPRLTIN
                     VNIGAGSLTLRNR"
     gene            complement(11100..11651)
                     /locus_tag="AC16CB_07460"
     CDS             complement(11100..11651)
                     /locus_tag="AC16CB_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342343.1"
                     /translation="MNNPILFISPKFQKRVLALSILASLALMALLNVINRPLISSASP
                     YGIISFELAGTLDAARNILAGWGETDRLIAAFGLGLDFVFLLAYPLAIGLACSWIAEE
                     LALYHHPRAILGIRLAWAQVLAAVLDAIENISLMEVLLGVEIALWPRIAQICAVLKFV
                     IVISGLIYALYGVVSVVVIRRRK"
BASE COUNT         2997 a         3418 c         2917 g         2555 t
ORIGIN      
        1 cctgatccag gcgcgaaacc aagctgatca aatgcccgct ggagcgcaag gctcgtatgg
       61 cattttccag cacctcatct ttgaccagga tgtaatcggt gctgtaggtg gaaagagcaa
      121 agatggacac cccagcctgc gctaacgtgg ctgccagttc cgccagcaca ccaaccaagg
      181 aaaaatccag cggaccgctg attttcaggg cgcgccaccc ctgctcagcc tgaacccccg
      241 gtggtacaaa ccgctgggca cacactatag tcagctcatc gccatcgcgt atcacggcca
      301 gcaaatcgct ctgcccagcc catgggggca actcctcgtc cggcgaaagc tggcacacag
      361 ccagcagttc aggcagtacg ctcagctcca tcagaagaga tacacctcga cctgctctcc
      421 agcttctaag ccggtggcat ccgcaggaat acgcaccaat ccatcggcgc gtgccagcga
      481 gaagatcaaa ttactcttgc caaagaccgg gtcagcttga tagccgccag cgccagggac
      541 caaccgcacg ggtatccaat cttcccggcc tgcctgggag gagaggttta gtgtcaagcg
      601 ggctgaagcg ctggggcgtg gttgtcgata tgcaagtccg agcagcgcct caaccacggg
      661 tatcacaaac aaccaggcga tcaccagggc gctcactggg ttgccaggca atccgatgac
      721 tgctttattg ttgcatacag ctaagatcgt cggcttgcca gggcgcacat tgaccccatg
      781 caccagcacc cctggcttac ccaggctgtt gaccacctgg gatgtcaggt cgcgcgtgct
      841 ggcagaagaa cctgctgtga tcaccaccac atcgcattca gccaaagctt tggaggcaac
      901 ttcccgcatg accttctcct ggtcagacac gatgccatag cgcacaggct ggccgcctgc
      961 cgcttcgacc agagaactca acgtgtaggc gttgacgtcc cgtacctgtc ccgggccaag
     1021 ggtcttctct ggggacacca cctcatcgcc actggaaatg atcccgaccc gcggcctacg
     1081 agacacctgc gttttgatca tacctaaggc catcaagccc ccgatctcag cagggcgtat
     1141 ccggacgcca gcaggtatca cctcatcgcc gggcaaaacg tcttcaccaa ccttgagcac
     1201 gttctcacca gcagccaccg cgcgcagcac ctccacttcg ccagggcgcg cgctctgggt
     1261 atgctcaacc ataaccacgg catctgcccc ttcgggaagc ataccgccag tatggatcaa
     1321 agcgcactgt gctgcagcca gtgaaaagcc cggcactccg cccataggga tctcaccaat
     1381 cagttgcaaa taagcgggca aggaatcgct ggcaccaaat gtatccccag cgcgtacggc
     1441 ataaccatcc acagtggtgc gcgggaaaga tggcaaaggg tgcggtgcaa ttacgggttc
     1501 taccgtcacc ctgcctaaag cctgttcagt gtcaatctct tccggctcag gatggatggg
     1561 taaatgcgct aatagctggg ctaatgcctc tgggggaggg agaagttcca aaaactcggg
     1621 catacttgat ggtcatccgg gtttgtcgat tcgtgaataa tctacgaatc acatactatc
     1681 ttgtccaaaa cgcgaaggtc aaaagatagg ttgtcgtcgc aaaaagcact accgcggtca
     1741 gtttgagcgc catccagttg ggcttgaccc cctcagcgat gcgccgcatc tgccagatct
     1801 gaagcaagcc cagtggcaaa gtcaacaacg ctggcaagcc aatgaacagc ggcatgccaa
     1861 aggtaagggc caggccaatc agcagaaacg caacgaaaat catgccgttg tgcagcccca
     1921 tcccgcgctg ccaacccagg cgcaccatta aattgcgccg atcttgctta atatctattg
     1981 catagtccgg caactcgaat gccatgatca aagccagcat caaggcagtc agcggaaagg
     2041 tgctcatcgc cagcaaacgg tgtaaatcac cttcttgcag aacaaaggca aatgcgggca
     2101 gcaaattagc atacaagatg gcgataacca attctccgta ccccgagtat gccaggcgga
     2161 ccgggggtac agaatagaac accgcgccca ggcacgccag tcccatgacc agccaggcta
     2221 ccggtgatag gcgcaactcg cgtaacatcc acacgctcaa cgtggcaacc actgtcaggc
     2281 ttactgcagc tgtcaccagc atggtgacag gtgttcttat tggaccaatc ttcccatccc
     2341 cgcccgacaa tggatcgcgg taaggattgc tcgcctcact aactgctgtg taatactcat
     2401 acagaaacac cccactcaaa tgcagggcga tcacccacaa ctgcccgact agataaacac
     2461 tccaatcgat gctcgtgccc aggtaatcag ctaccccagc gcccaaggca tagattaaga
     2521 cagcacctag caggaagagc gggcgcgaga tgcgaaaaaa taggacaaac ggattttgcg
     2581 aacggctatc agccatcttg ttcgggttcg ctggaattca tcatcctgcg gatttctctg
     2641 aggtggatct gattatggcg gtacatcatt ttcaagatgt ccgcaataga tgccatgccc
     2701 agccagggat gtcgtcctgt ctttgtcagg tcatccgggt tcatgttggc tgccagcgca
     2761 gcgctttttt ctcgaagaaa ggcaaattgc cctaacagct cgtctgtgga ggcttccttc
     2821 aagctggcta ccttgcgctc attataggca ttgagatcaa aatcatcagg tgacccaagc
     2881 cctcccgcca ggatattctc cacaagacga gtcatccccg actcggcaga cacaaaatgg
     2941 gccaatacct cccgcacggt ccacacagaa ccttctgtgt acatgatggc gcgccactgg
     3001 tctggcctta actcgcggaa aaactcaagg gttcttttac cctcgacctg catgcgctcc
     3061 gcgatacggg tgggcgagtc agtcattcga atgttcctta tggctccatg ggatagccag
     3121 cgttggacca ggcgacgaat ccgcctttga caggcatgac tgaattgaag ccgttgtttt
     3181 gcagtagttg cgccgcacgg gcgcttgact cttcagccgg tcaggttcag taagtgatga
     3241 tccagtctga cggattgagt tcactgatcc ggttgggcag ctcagccaaa gggatagata
     3301 atgccccggg gatgtgactc tgggcataag aatcagcgct gcgcacatct acaaacacgg
     3361 cgctacctgt atcgtaggct gccttggcat ctgcgaggct gacgcgggct atatcagggt
     3421 aattttcttc ttctaccgga gaactgggtt gaactaactc tggagggctc aaagtcatgt
     3481 tgtagtacca cacgccggca ctcaagatga gcaccaaacc cacagctacc agaacaaacg
     3541 gcaagatgga aggagaggta cgtctttgtg gcatgaaacc tccattgggg gattaacgat
     3601 caactatacg ctatcagcga gatgatacca taaatccccc ttgcttcaga ttaaccccat
     3661 ggataaaccg gggcaaacgc atttgggcga tttcccacca ctgaggagag aaaccagggt
     3721 gccgctacct gcgaaagaaa ttcgagacaa agaaccagag gtttgcagga cgctcagcca
     3781 agcgacgaat gaaatagggg taccactctg tgccatatgg aatgtaaatg cgcaccgggt
     3841 agccatctgc agccagtttt tgttggagat cacgacggat accgtgtagc atctgaaact
     3901 ccatcgcctt cttcggcaat ccaatttgct gcgcgtagtt taaggcattg atcacgcgtt
     3961 gctcatcatg ggttgcaaaa actggcggag gaggaatcct accgtcatcg ctgagctgcg
     4021 ggcagcctcg ttgcaaggca ctatccatca tcaggcgtgc caggagatca tagttggcat
     4081 ccacgtcctt tttccttgga aaagccacat cgggtggttc tttgtaggcc cctttacaca
     4141 gacgcacgcc ggtacactcc tcgcacaggg aatgaatatc ctgctcgctg cggtacaggt
     4201 aagactgaat gactacgccg agatgttcga agccatgctc gaggtgtgcc ttccggtaga
     4261 gagccagagt cttatccacc cagggcgaat cctccatatc aatacgaatg taggtaccca
     4321 cctgtctggc tcgctccaag atggtcatca agttctcctc gcacaggctt tcgtccagcg
     4381 caaggccaat ttgtgtcagt ttaatcgaca cataggagcg cacaccagtt tgctggattg
     4441 cgtctaaaat cctcaggatg tcttgtgtcg cccggcgagc ctcgtcggga ttggtggtgt
     4501 gttcgcccag atgatccagc gtggcgttga tccctttttc atttaacttt tttaccacat
     4561 tgatcgcatc attcagagtc tcccctgcga tgaagcgtga ggcggccttc caagcaatac
     4621 tccagcttgt gacaattttc cgcgcccaag cggcttgtga gagataaatg aggaaggagc
     4681 gaagcatatc tgaataccct catgttgctg ttaatgatac gaaacccaac ccctcggggt
     4741 cggcaatgtc agtctcatgc agaaatttga tcaagcgaat ccattgtagc ataaatcact
     4801 tttgaagtct gtttacaagc atcctgcctc cagccgacaa acttcacatt aaaggtcaac
     4861 tctgatcaag caaatgaggg agatttgtgc gaagcaagcc gtctaatacc ctaatccgtg
     4921 ttcagcttta ctgcttgcat tcacctatcc ctggcacatt tcttgcaaca ggttataatc
     4981 agatattgca caaaaggaaa aaacatgctg caacagtccc aggctcctat cacccgcccc
     5041 ataaccaccg ctcacctggc gacgacgatg tcgttgatga gcttaaccgc catcgagttg
     5101 cacgagcgaa tcgaggcgga gctagcctcc aatccggcct tggagctggg tgaaaaacat
     5161 cactgcactt cctgtgggcg tcccctggtt gagaaaggaa cctgcctggt gtgcagccgg
     5221 ctctcaccag ggttagggga agaacccatc gtgttcatct ccccgcgcga agacttctat
     5281 ctcccggcac gccaaagcac acccaatgga atacccggcg acgagatcac cgccgagaca
     5341 gaagatttgc ccacctatgt cctgcgtcag atcgcccctg agctagcccc cgaagaccgc
     5401 ctgctggcag cccacttgct caccagcctg gatgaagacg ggctactgcg cgtccccctg
     5461 atcgagttcg cccgctacca tcaccgaccg ccttcccata ttgagcaact tttaaagctg
     5521 atccagcgcg ctgacccgat aggcgttggc agctcatctc ccgaagaagc aatgctggtg
     5581 caacttgaag tcctgggaga aacgcagtcc atccccccta tgaccgacca ggtcatccgc
     5641 cagggcatag ggttgctctc acgccgccac ttcacggagt tgggcaaatt gctgggcatc
     5701 tctgcctccc aggtcaaaca aatcgctcgc ttcatcagcg gtaaccttaa cccctatcct
     5761 gcccgctccc actggggtaa tgtccatcaa ggtaccgaac cggcacagga cgtatatcac
     5821 ttccccgata tccttatcag ccggctgaac gacaaccccg actcgtccct catcgtcgaa
     5881 gtgatctccc ctttggcagg caggttgcgg ctaaacttac tattccgaca atcgatccgt
     5941 ctcgcgcctg aagacaaggt cgaggagtgg cagaacgact tggagcaggc atccttactg
     6001 atcaaatgcc tgcaacaacg caatcacacc atcgtgcgct tgatgcagcg aatcgcggcc
     6061 atccagcggc atttcatctt acacggtgat gcttacctgg agccgctgac acgcgccagc
     6121 ctggctgaag aactccaggt gcacgaatcc actatctccc gcgcggtagc cggaaaagcg
     6181 gtacaattgc ccaatggcaa gatcgtgcca ctctcgaaga tgttcgaccg cagtctgcac
     6241 atccgcaccg aactgcgcca gatcatcgcc catgagtgcc aaccactcac ggatacggag
     6301 cttgcggcat tattaaagaa aaaaggctac cgggtggctc gccgcaccgt agcaaaatac
     6361 cgtgctatgg agggcattct gccggctcac ttacggcaac ccctgcagac cgctggctag
     6421 tggattgatg aataccccac tcaaactcaa catgctcccc ggattgcgcc ccacaccagg
     6481 actggccgag attgagagca tgatggagct attcaaatcc gcctgtcttc tggtggaatc
     6541 tcgcaatcga cgcatcctgc tggccaattc ccaggccaca gagctgaccg cctacactcg
     6601 aggcgagttg tctgggatgg ctttttccaa gctgattgac caacccaata agcatccttt
     6661 ctgggaatcc ccctctggac aacccttccc tactgcgcta actctgctta gacgtaatgg
     6721 ggcaaaagtt agcgtgcaag cgacgcgaca cgaactggca gcacaaagca aatgggccat
     6781 catccaattg gaagaatcgc atgtaatcga gcaacgccag agccagcagc gccgacagtc
     6841 ggggatgata cagagcatgg ttaccatcag tcaggccatt gggctgcctg atctaagcga
     6901 agccatcaca atgattttag aagcaagcca tgagattacc ggttgcgaca acatcggtgt
     6961 ctatcttcaa gaaatggcca ccaaagggca aggctttgag atggttcgct atgcccagca
     7021 tggcgcagat aatatcctac ctgagcgctt acctgcccaa gatctggtgc atctacgtgc
     7081 ccccaattac tggattcctg ggaagcgttc tgcctccagc ctacaccggg cggctcgcag
     7141 cgctgggttg gcctttctgg cttctgcccc attgggacag gataaagccc cggttggctt
     7201 tattgccata gccgggggtg ccccgatagc acaaaatgaa atcttgcctc ccttggagat
     7261 cctagcgaat attatcgatg tgctgatcca aattcacaca cgcaccacaa atttagaaaa
     7321 atctcttggc gacaacattc gcgctcgcgc attagagaag accagcatgg atgccattga
     7381 agacggcata attgtactca caccgacact caatgtggtg cggctgaatc cagctgctga
     7441 gacaatcctg ggatacacaa accgggaggc gcgtaaccgg ccagtcgaag acattctcat
     7501 cggtactggt gcgttaacca cctccctgga gctcgccctg cagggcgtac ccaccatcaa
     7561 acaagagaat ccacacctct accgtcgttc tgggacaccc ttccttgccc aggttagcat
     7621 cataccagca catgtggaag atcatttaga gggcatcgtg gtcctgatac gggacttgag
     7681 cgaacaggag caaatccagg tccaggcaga gcaactcaaa cagcgtgctg tgcttggcga
     7741 ggtatcctca atcttcgccc acgaggtgcg caatcctatc aacaacatta gcactggtct
     7801 gcaagtcatg gcctacaact tatcccccga cgatcctcat caggaggtga tctcccgctt
     7861 gcagcgcgat tgtgagcgcc tggatgaatt aatgaaatct gtattgacca tcggtcgccc
     7921 agcggaatac aaattgggtc caactgacct gagcatgctg gcaactcgtc tggtggagcg
     7981 ttttaagcca cgcatggtga acgccaatat tcaaccattc cttcagattg agccttcctt
     8041 gcctctggtg aatggcaact tgctcgcctt ggaccaggtc ttcatcaacc tgatcaataa
     8101 cgctattgag gcgatgagcg aaaaaggggg caccctggca ttaagattgc agaccgccac
     8161 agatggtgat ggcaaacgct tcgtagaggt caatgtagct gacaacggtc ctggcattcc
     8221 taaagaggta caggaacgcc ttttccaacc tttcttcacc accaaaaaag atggcaatgg
     8281 cctaggtttg gtgatcaccc gccgcatcct aaatgcccat aaggggacaa tcaccctcac
     8341 cagctaccca ggcgctacca tatttcacgt acaaattccc gttctggagt cgccatgagc
     8401 ttgaccgttc tgattgtaga tgatgaagaa aatgcccgct ataacatcag ctcgttcctt
     8461 ggaggccgcg ggtacgaagt gttgggtgta tccacattaa gtgaggcccg cgagacgttg
     8521 cgccgcggta atgcggatat cattttgctc gatgtaacac ttcctgatgg attcggcacc
     8581 gacctacttg aagaaaccgc acatatgccc aatcgccccc cgattattat gatcacggcg
     8641 tatggcaata tcgagatggc ggtaaatgcc atgaaaaacg gcgcgcacga tttcttaccc
     8701 aagccaatcc agcttgctga actggagaag tcaatccagc gcggcgggga actcgttgcc
     8761 ttgcgcagag agttggcaca cctgagatcg gttcagcaca accaggttga ttttgtcgtt
     8821 gggcagacgc cggtgatgcg aaacctactt aaccaggcac aacgcgccgc ggctgcatcg
     8881 gtctcggtcc tgatcaccgg agagacaggc acaggcaaag aagtattggc gcgcgccatc
     8941 cacggcatgg ggccacgctc cagcaagccc tttatcggtg aaaactttgc cgccatccaa
     9001 cctacggttt tggaatcgga gttgtttggc tacgagccag gggctttcac gggcgcggag
     9061 aagcgcaaga ttggcctgat ggaagtggca gaaggtggca tcctgttcct ggatgaaatc
     9121 tcctccttat cagtggatat gcaagccaag ctgctgcgcg ccttagagga gcgcgccttc
     9181 agacgggttg gcggcaacaa ccagattcgt gtggatgtgc aattcctggc agcctccaac
     9241 cgtcacctgc ccacactgat taccgaaggc aagttccgcg aggatcttta ctatcgcttg
     9301 aaggtggtag acctgcacct gccaccgttg cgcgagcgca aggaggacat tcccgagctg
     9361 gtaggattgt ttatacgcca gctcaaccca cgtatgggac ttaatatcac ggacgtcaca
     9421 ccgcgcgccc tacaggctct tatgtctcat aattggcctg gaaatatccg cgaacttcgc
     9481 aacatcattg agcgagccat gcttttctgt gatgatgcag ccatcgattt gccccacctg
     9541 cctgctgagt tatcgaacgg taaagaataa tttgcgcagt cgagcgtgga ttatctcgta
     9601 cctttatttg atttattttc tggtaaaata aaccctatcc tgttcaacac accaccattc
     9661 catccacgga ggtttgatct atgacctata tcatcaccag tctatgcctg cgcgacgccg
     9721 gttgcgttga agtctgcccc gtcgagtgca tcgtgccagg aaaaccgcaa gaagaatggc
     9781 cgtggtatta tatcgacccg gatacctgca ttgactgcgg agcgtgtgtc ccggagtgcc
     9841 cgtatgaagc catcttcccc gaagatgagg tgcctacgga ttacactgcc aagggcggag
     9901 aatatgtcag caaagtcggt ctaaccggac attatgaggg taccaaccac cacgacgaag
     9961 cggttgtcct tgattccgtc cgccagctag aagccggcga agtggttgat ttgaccgccg
    10021 acaaccaagc caattacgat tatttcacat ctggcccagg ctacagtgcc ctcgaataac
    10081 aaggctggat aaatattcaa aaaataaagt ggcaggccta acgcctgcca ctttatttat
    10141 ggtctctaaa ggggttaccg gttcctcaat gtcaggctcc ccgcgccaat gttgacgtta
    10201 atggtcagtc gaggaccttc acctgacaag gtatattcat tcccagactt ctcccactcc
    10261 ccgccgatat ctacattcgc taaaccgcta tccacaaaca cgcgtgtcaa gactccctcg
    10321 ggcacaatca gcttgacgct gctaaaaccc gaatcgatcg tcacggtggc gtcgcgcttc
    10381 agctcgcccg agaagtcgag tgtatagtcg ccagcaccac ctttgaatac catgctgtcg
    10441 aaattggcat ttgccagacc agtcatctcg actcgggagg cgcctgtggt gtagcggaat
    10501 gtatccatct cggatttatt cggcacagag aagctcaatt gagcatctgc agcgccatcg
    10561 ctgacgcgca gcgattcgat tttcagacca cccagctcga actcaccctt gtatgcccct
    10621 gcattgatac gcaggtccat agggacatcc cccaatctta agtcccacac gttcttatac
    10681 tcatcaccga atctgggtat accgtcaatt tcaaaatccc cggtggtgat gagcacttcg
    10741 ttcttgtcaa cactcacctg aggcttcaaa tcccgaacat tatagcttgc cgtgccattg
    10801 accagggcat cctgggcgcc tggagcaagc gtgaattccc ccgcgccaaa tgaaaactcc
    10861 actcgcgtta catctccctg ttgctctggt agggagacag agacatcctc ggtcactaag
    10921 ggaccaatat tgacctcttt gaccggcaca tcgatggtaa atccacaggc caaggtgacc
    10981 agggccagag cgctaagagc aattaacata gattttggta acataataat tctccatttt
    11041 tatagtatgt caatttcata tacgctcaat tggtcgcgag gttgcaaatt aagaacgagt
    11101 cattttcggc gcctgatgac aacaacactt accaccccat aaagggcata aattagccct
    11161 gatatcacga taacaaactt taacaccgca catatttggg caatacgcgg ccatagcgcg
    11221 atctcaacgc cgagcaaaac ctccatcaag cttatgtttt cgatagcgtc caatacggct
    11281 gccaaaactt gcgcccaagc taatcgaata cccaaaattg cccgcggatg atgatataag
    11341 gcaagctcct cggcaatcca ggaacaagcc aacccaatcg ctagcggata cgccagcaag
    11401 aacacaaaat ccagccccag accaaaagcg gctatcagcc tatctgtctc tccccaaccg
    11461 gcaagaatat tgcgggctgc gtccaatgtg ccagccaact cgaaagaaat gatcccatag
    11521 ggggaggcag atgagatcaa tggtctatta atcacattca gcaatgccat caaagccagg
    11581 gaggctaaga tagaaagggc cagaacacgc ttttgaaatt ttggcgagat gaacagaatg
    11641 gggttattca tagaccgctc ctcctatggc tcaaaaagta ccattaattg ttttcctttc
    11701 agagtatgcc ataggaacac ttatctcaca agaagatcgc gtctactttt aagataaaat
    11761 gatatgaatc acttctaaaa ttcataaagt caccgtgtaa atacaagagg atacccatga
    11821 ccgacaaccc acaacctgaa aataatctat ctgacgagtt tcgcgcctta ggggaaaacc
    11881 taatcaa
//
LOCUS       DBNGSX010000243         2135 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1889595, whole genome shotgun sequence.
ACCESSION   DBNGSX010000243 DBNGSX010000000
VERSION     DBNGSX010000243.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1889595"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..320)
                     /locus_tag="AC16CB_07465"
     CDS             complement(168..320)
                     /locus_tag="AC16CB_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342344.1"
                     /translation="MNVCIILSTPLPPREGIGDYFTQPVSGFDIAVTLAGARPLIICD
                     SLLTHA"
     gene            complement(317..832)
                     /locus_tag="AC16CB_07470"
     CDS             complement(317..832)
                     /locus_tag="AC16CB_07470"
                     /inference="COORDINATES: protein motif:HMM:NF016027.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3342345.1"
                     /translation="MIFVTVGTHFQGFERLVRAIDELAAHLDEPMVIQYGCSSYIPRH
                     AEGFQWASSQRMEQLTRQARLVITHAAAGAIILALRFGKPVVVVPRRKAFGEQFDDHQ
                     EQLAEALDRAGWAIAVSDPTPQSLQAALPRANGLDLKRNASAAPLVQAVHAQLATWAS
                     AKDRRLKENVR"
     gene            complement(829..1278)
                     /gene="pssD"
                     /locus_tag="AC16CB_07475"
     CDS             complement(829..1278)
                     /gene="pssD"
                     /locus_tag="AC16CB_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssD/Cps14F family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR3342346.1"
                     /translation="MKVALVCTQGGHLTETLQLLGAFKGHQIFFVTHHSPRDEDLNAI
                     ATTYLTVSIEAHPMRLLYAFIEALPILFREKPDVILSLGAEIAIPFFFWGKLLGMKLI
                     YLESWCRVSNLSRTAKIVYPIVDEFWVQWPELLEVCGPKAKFHGAVI"
     gene            complement(1291..>2135)
                     /locus_tag="AC16CB_07480"
     CDS             complement(1291..>2135)
                     /locus_tag="AC16CB_07480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3342347.1"
                     /translation="GRSIGWETSVYRSSMALTRSTLPKISIITPSYNQGRYIEHTIRS
                     VLDQGYPNLEYLVIDGGSTDETLDILKKYDGALTWISEQDRGQAHAINKGLKRTTGDI
                     VAFLNSDDLYEPDALLQVGEYFVAHPKASFLTGRCRTIDEHGREIRKWITRYKYLWMR
                     LHSYQILLVLNYISQPATFWRKAVTDVIGFLDEGLHYTMDYEYWLRIGKTTPIHCLLT
                     FLASFRLHSNSKSASTAHLQFDEELQVARLYGGKSHLFLHQLHRNITVLIYQKLLEKH
                     GLPA"
BASE COUNT          510 a          513 c          596 g          516 t
ORIGIN      
        1 tatctgggga gaatcgaccc aattcaacta ttaaatctgg ggtacagtct ttgtggtagg
       61 gtatttgcaa cgcttgatat cttgatttga tatcacttgt ttgtcgttga aaatttggcc
      121 gcaggtcgat tatttcaaaa gtaacaaaat cgtgaatttt gaatagatca cgcatgagtt
      181 aataacgagt cgcaaatgat cagtggacgt gctccagcta aggtgacagc gatatcaaac
      241 ccagatacag gttgtgtaaa gtagtcaccg atgccctcgc ggggcgggag tggggtggag
      301 agaattatgc agacgttcat cggacattct ctttcagtct gcgatcctta gcgctggccc
      361 aagtcgcaag ctgcgcatgc accgcctgga ccagtggcgc cgcgctggcg ttcctcttga
      421 gatctaatcc gttagcgcgt ggcagggcgg cttgcagcga ttgtggcgtg gggtcggaga
      481 cggcaattgc ccatccggcg cggtcgagcg cctcggcgag ctgctcctgg tggtcgtcaa
      541 actgctcgcc aaaggccttg cggcggggga cgaccacgac aggtttgcca aaacgcaggg
      601 ccaggatgat cgcaccggct gcggcgtggg taataaccag gcgcgcctgg cgcgtcagct
      661 gctccatgcg ctggctggag gcccattgga atccctcggc gtggcgcggg atgtaagagc
      721 tgcatccgta ctggattacc atcggctcgt ccaggtgggc tgccagctcg tcgattgccc
      781 gcaccaggcg ctcgaagcct tgaaaatgag taccgacggt aacaaagatc aaatcaccgc
      841 tccgtggaat ttagccttgg ggccgcatac ttcgagcagt tcaggccact gcacccagaa
      901 ttcgtcgacg attggataga cgatcttcgc agtcctggag agattgctta ccctgcacca
      961 gctttcaaga tagatgagct tcataccgag cagttttccc caaaagaaaa atgggatggc
     1021 gatctcggct cccaggctta gaatgacatc aggtttttcg cgaaacagaa tggggagagc
     1081 ctcaataaag gcgtataaaa gtcgcatagg gtgcgcttca atgctgaccg tcaaatacgt
     1141 ggtggcaata gcatttaggt cttcatctct agggctgtgg tgggtgacaa agaatatctg
     1201 atgccccttg aaagcaccta ataactgaag cgtctctgtg agatgccctc cttgggtgca
     1261 aaccaaagct actttcatat atcaggagtc ctaagctggc agcccgtgtt tttctaatag
     1321 cttttgatag atgagcacag tgatattgcg gtgtaactgg tgtaaaaaga gatgggattt
     1381 gcccccatac aggcgggcaa cttgcagctc ttcgtcgaac tgcaggtggg cggtcgaggc
     1441 agatttggaa tttgaatgca ggcggaaact cgccagaaag gttaacaggc agtggatggg
     1501 ggttgtttta ccgatccgaa gccagtactc atagtccatg gtgtaatgca agccttcatc
     1561 caggaagccg atcacgtctg tgactgcctt acgccagaag gtagctggtt gggagatgta
     1621 atttaataca agcagaatct ggtagctgtg caatctcatc cagaggtatt tatagcgtgt
     1681 gatccacttg cggatctcgc gcccgtgctc atcaattgta cggcagcgac cggttaggaa
     1741 ggaagctttg gggtgcgcca caaaatactc tcccacctgc aataaagcat caggctcgta
     1801 caaatcatcc gaatttaaaa acgccacaat gtcgcccgtg gtgcgtttca accccttgtt
     1861 gatcgcatgc gcctgtcctc gatcttgctc gctgatccag gtcaatgccc catcgtattt
     1921 ttttagaata tctaatgtct cgtccgtgga tccgccatcg atgaccaaat attcgagatt
     1981 gggataaccc tggtctaaca cggagcggat ggtgtgctcg atgtagcgac cctggttgta
     2041 tgatggggtg attatagaaa tcttaggcag agttgagcga gtcaaagcca tggatgatct
     2101 atatacggag gtttcccagc cgattgacct acccg
//
LOCUS       DBNGSX010000244         2152 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_711783, whole genome shotgun sequence.
ACCESSION   DBNGSX010000244 DBNGSX010000000
VERSION     DBNGSX010000244.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_711783"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="AC16CB_07485"
     CDS             complement(<1..928)
                     /locus_tag="AC16CB_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR3342348.1"
                     /translation="MELCDLPAHILAENIRLRQVKAIDALEAALARIAAVDGDPPTLE
                     PADLDSDDHVHAFITVTAERARLQAREIDRRLDAGEDVGPLAGVPFTVKDIFCVKGTP
                     STAGSRILANFTAPYTATPVERMEAAGGVMLGKVNLDEFTYGSSNESSAFHPTPRNPW
                     NVQRVPGGSSGGSAAAVAAGEGALSLGTDTAGSIRQPAAFCGVVGMKPTYGRVSRYGL
                     IAFGSSLDCPGPVARNVTDAALMLKVIAGADRRDATAAKVSVPDYLSDLKRGVRGMRI
                     GLSPDYFRITYFDTSTGELHQEPIAPEIEDALL"
     gene            complement(1026..1343)
                     /locus_tag="AC16CB_07490"
     CDS             complement(1026..1343)
                     /locus_tag="AC16CB_07490"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF014716.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR3342349.1"
                     /translation="MTEPILPDLFNHLVQLAALELSPEEAEYLRRELNNQLKAIHELE
                     AIPLGADIPAASHGVSYTEDTSPAIRQDEWHPHPNPDEILEQAPETDEGYIIVPDIPH
                     TEL"
     gene            1528..>2152
                     /locus_tag="AC16CB_07495"
     CDS             1528..>2152
                     /locus_tag="AC16CB_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005996868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3342350.1"
                     /translation="MIRQAARDFAQKEIAPIAAHFDETGEFPYTTIKKMGEMGFMGVE
                     VPEEYGGPGMDTLAYVLALEEICKADASHGTIMSVNNSLFCHGLNKFGTEEIKQKYLR
                     AVASGEAVGAYSLTEPMSGSDAGNMRSRAVREGDHYVLNGRKSWVTSAPVAEYIVVFT
                     MTEPEKKHRGITAFLIESNKPGFQQGKKEPKLGIRASATSEIVFEDYK"
BASE COUNT          523 a          575 c          623 g          431 t
ORIGIN      
        1 gcagcaacgc atcctcaatc tcgggggcaa tcggctcttg gtgtaactca ccagtgctgg
       61 tatcgaaata ggtgatacga aaatagtccg gtgacagtcc tatacgcata ccgcgcacgc
      121 cgcgcttcag atcagacagg tagtcgggaa ccgatacttt cgctgctgtg gcatcccgcc
      181 ggtcggctcc agcgattacc ttcaacatca aggccgcatc cgtcacgtta cgcgccactg
      241 gcccggggca atccaaagac gagccgaaag cgattaagcc atagcgcgag acgcgcccgt
      301 aggtgggctt catacctacc acgccgcaga acgcagccgg ctggcggata gagcctgcag
      361 tatcggtacc cagagaaaga gcaccctccc cggctgctac cgccgcggcg ctaccacccg
      421 atgagccgcc cggcacgcgc tgtacattcc aggggttgcg cggggtggga tggaaagccg
      481 acgactcgtt ggatgagcca taggtgaatt cgtccaaatt taccttgccc agcatcaccc
      541 caccagcagc ttccatgcgt tccaccggag tggcggtata tggcgcggta aagtttgcca
      601 gaatgcgcga gccagccgtt gaaggtgtcc ccttcacaca aaagatgtct ttcaccgtga
      661 atggcacgcc tgccagcgga ccgacatctt caccggcatc cagccggcgg tcgatttcgc
      721 gagcttggag gcgggcacgc tccgccgtta cggtgatgaa agcatggaca tgatcgtcag
      781 aatcaaggtc cgcaggctcc aaagtcgggg gatcgccatc cactgcagcg atgcgggcca
      841 gcgccgcttc cagcgcgtcg attgctttca cctggcgcag gcggatgttt tcagcaagga
      901 tatgggcggg taagtcgcaa agttccatgg gtatgtttca ggtaacaaga tctaggttct
      961 aagtaccaag ttccaagttc caataagcag gtgaacgtgg cacttgacac ctggaacact
     1021 aaaaatcaga gttcggtatg gggaatgtct gggacgatga tataaccttc atcggtctca
     1081 ggggcttgtt cgaggatctc gtccggatta ggatggggat gccattcatc ctggcggatg
     1141 gcaggggagg tgtcttccgt ataggaaaca ccgtgtgaag cggcagggat atcagctccc
     1201 agtgggatgg cttccagttc gtggatggct tttagctggt tgtttagctc acgccgcagg
     1261 tattcagcct cttcaggaga aagctcgagt gcggcaagct ggacgaggtg gttaaaaagg
     1321 tcggggagta tcggctcagt cattcatcaa gctccaattt gggttagctc actccgcaag
     1381 gcagtatatc cgactttgag tgccaacgca agcgaaaaga aacgtatatc atctagaggc
     1441 atgctataat gaatctggca gcagctttga tctaaggagg agaatatgac caactacggc
     1501 ttattccagc ttagccccga gcatgaaatg atccggcaag ctgcgcgtga cttcgcccaa
     1561 aaagagatcg cccccatcgc cgcgcatttc gacgagacgg gcgagtttcc ttatacaacc
     1621 atcaaaaaga tgggggaaat gggctttatg ggggttgaag tgcccgagga atacggtgga
     1681 cctggaatgg acacactggc ttacgtgctg gcactagagg agatctgcaa agcggatgca
     1741 tcacacggca caatcatgtc ggtgaacaat tctctgtttt gccacggctt aaataaattc
     1801 ggcacggaag agatcaagca aaaatacttg cgcgcggtag catccggaga agccgtgggc
     1861 gcttactcgc taaccgagcc gatgtccggc tcagacgctg gaaatatgcg cagccgggcc
     1921 gtacgcgagg gcgaccatta tgtgctcaat gggcgcaaat cgtgggtcac cagcgcgccc
     1981 gtggcagagt acatcgtggt ctttaccatg accgagccag agaagaaaca ccgcggcatc
     2041 acagcattcc tcatcgagag caacaagccc ggttttcaac agggtaagaa ggaacccaaa
     2101 ttgggaatcc gcgcctcagc caccagcgag atcgtctttg aagattacaa gg
//
LOCUS       DBNGSX010000245         2234 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_296517, whole genome shotgun sequence.
ACCESSION   DBNGSX010000245 DBNGSX010000000
VERSION     DBNGSX010000245.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_296517"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="AC16CB_07500"
     CDS             complement(<1..217)
                     /locus_tag="AC16CB_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342351.1"
                     /translation="MAALRLPRRPHPAAGNGGRQSAPAAAGKPRPSAGGLHFGGKVPI
                     VPPEPPDTRPPPRGTGHDDTVGFREPGR"
     gene            196..984
                     /locus_tag="AC16CB_07505"
     CDS             196..984
                     /locus_tag="AC16CB_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342352.1"
                     /translation="MADEGPPPITLFPPPFDTTLHGLTPGQWYKFWMQPYNNLGDIGP
                     AVSFEKMILDYTDGDFDELPDQWEAIFGVSDPSADEDEDLLINLHEYELGSDPLNADS
                     DGDGFYDSEEANPKFGLAISDPCGPDTPPAHEGPKLAVVGLAEMNFTSAVNLPPPPQY
                     VQVMNLGGGALNWTVAASHSWIVLDEPAGDGYGAIPIRVDPKAVRSYGTHAGQITITG
                     EPGGRTAPRAALVDNQIVEKVTIQVEMTVLPPKLLELYLPMIMR"
     gene            complement(1149..2054)
                     /locus_tag="AC16CB_07510"
     CDS             complement(1149..2054)
                     /locus_tag="AC16CB_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342353.1"
                     /translation="MDASEIIQNIVITTISTTISAIIGGGVKIVFEIIRYRIERERRQ
                     EHYTDQASQATTGQSSRSLLTRSSILKFSIFAASIGFVGSLVFTSARPIGVSVGTNNT
                     TNPIAIPPRGSLIMDINFDQSGDGNCNDYDRDRLGYKNEKYYIQPELHRAYIAICHEI
                     EETDPYGDLQVSAYPVGNLTPDDTYGYGLLFGWDGGGRSTSEACIAGILRYKGNTFAY
                     FTDVVNGLWKGDYVPVKTIELDTAQHVLRVVLQKDGLAVVILDGQALAEHRFTICRPG
                     PIGISAWNSLGNKVYFDDLSLYALP"
BASE COUNT          540 a          588 c          554 g          552 t
ORIGIN      
        1 ggcgtccagg ttctcgaaat ccgacggtgt cgtcatggcc agtccccctt ggcggtggtc
       61 gggtgtcggg tggttccggc ggaactatag gaactttgcc cccaaagtgc aacccgccgg
      121 cggacggacg gggcttgccg gcggcggcag gcgcgctttg ccgccccccg ttgcctgctg
      181 ccggatgtgg tctacgtggc agacgaaggg ccgccaccga tcacgttatt ccctcctcca
      241 ttcgacacca ccctgcacgg gctgacgcca gggcaatggt acaagttctg gatgcagccg
      301 tacaataatc tgggggatat cggtcccgcg gtcagtttcg aaaagatgat cctcgactat
      361 acggatggag atttcgacga actccccgac cagtgggagg cgattttcgg ggtgagcgac
      421 ccgagcgcag acgaagacga ggatttgctg atcaacctgc atgagtacga gcttggctcc
      481 gatccgctga acgccgatag cgacggggat ggcttctacg acagcgaaga ggctaatcct
      541 aaatttggct tagcgataag cgatccatgc gggccggata ctcccccagc tcatgaggga
      601 cctaagctgg cggtggtggg gctggcagag atgaatttca cctcggctgt caacctgccc
      661 cctccgcctc aatatgttca ggtgatgaac ctgggtggcg gggcgctgaa ctggacggta
      721 gctgcctccc attcctggat cgtgctggac gaacctgctg gcgatggcta tggggcgatt
      781 cccatccggg ttgatccaaa ggcggtgcga tcatacggta cgcatgctgg gcagatcacg
      841 atcactggcg agccaggagg gcgaacagcc ccaagagctg cgctggtaga taatcaaatc
      901 gtcgaaaagg tgaccatcca ggtggagatg accgtcctgc ctcccaagtt gctggagttg
      961 tatctgccga tgatcatgcg ctgagctttg gttgctaatt taaatcgggc ttcataaaac
     1021 gagacaggag gcggcacacg accgcatcct ttctcgtttg tctctttgcg tgagtatata
     1081 gctcaagcaa cctttttata gatttacgta aaccccaaaa ggacacacca ctacctatct
     1141 ggctagtttc atggaagcgc atataaggat agatcgtcaa aatatacttt attccccaat
     1201 gaattccatg cgcttattcc aattgggccc ggcctgcata tggtgaatct atgctcagct
     1261 aaagcttggc catctaaaat aactaccgcc aggccatctt tttgaagcac aacgcgtaaa
     1321 acgtgttgag cagtatcaag ctcaatcgtt tttaccggta cgtaatctcc cttccataaa
     1381 ccattaacga catcagtaaa atatgcaaac gtattgccct tgtatcgaag aattcctgcg
     1441 atgcaagcct ccgatgtcga tcgaccccct ccatcccatc cgaatagaag accatagcca
     1501 taggtgtcgt ctggagtaag gttgccgacg ggatatgcac ttacctgcaa atccccatat
     1561 ggatcagttt cttcaatctc gtgacaaatt gctatatagg ctctgtgtag ttctggttgt
     1621 atatagtatt tctcgttttt ataccctaag cgatcacggt cataatcgtt gcaatttcca
     1681 tcaccgcttt gatcgaaatt tatatccatg atcaagctgc ctctaggagg tattgctatc
     1741 gggttggtag tgttattagt tcccaccgac accccaatag gccgagcact tgtgaatact
     1801 agactaccaa caaatccaat cgaagctgca aaaatggaaa attttaatat tgagctccgc
     1861 gtcaaaagag accgagaaga ttgaccagtc gtggcttgag aggcctgatc cgtgtaatgt
     1921 tcttgcctcc tttccctttc aatacgatat ctaatgatct caaaaacaat cttcacaccc
     1981 ccaccgatta ttgcagatat tgttgttgaa atggtggtaa ttacgatatt ctgaattatc
     2041 tccgaagcat ccatactccc tccccgtcga caagtacccc cgcaaacacc tatgcattga
     2101 gacaacaaaa cgtcaacatt gagttggcgg ctgtgttgtt ttctaaattg ttttgccggc
     2161 ctcccctgcc ggcctgccct ccgcctgcga tgttatacaa taggctgtga tttgagtata
     2221 caacagacga aatc
//
LOCUS       DBNGSX010000246         2310 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_850931, whole genome shotgun sequence.
ACCESSION   DBNGSX010000246 DBNGSX010000000
VERSION     DBNGSX010000246.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_850931"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..712)
                     /locus_tag="AC16CB_07515"
     CDS             complement(8..712)
                     /locus_tag="AC16CB_07515"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3342354.1"
                     /translation="MEVARIENVTRIYKTGKVETHALRGVSLSIESGEFAALVGPSGS
                     GKTTLLQLIGCLDKPTSGRVYVDGKDVSQLNRNQRANMRRGTIGFIFQFFALIPTLTA
                     YENVELPLLLNGHSSSERRARVMQLLESVDLTERAFHRPDQLSGGEQQRVAVARALAT
                     QPTLVLADEPTANLDTPNGKQVMETMTRLNQETGVTFVFATHDPRVIQYARRVITLRD
                     GVIVENSEAEEISLGA"
     gene            complement(782..1999)
                     /locus_tag="AC16CB_07520"
     CDS             complement(782..1999)
                     /locus_tag="AC16CB_07520"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3342355.1"
                     /translation="MIQLFKMAFRDLGRNRRRSFFSSLALALGLALLLLMAAFIQGEM
                     GSAMDATIRLQSGHLQIRAQSYDESKTSLKWEDLIENPAQVAEQLASLAPVKVATPRL
                     FASGIVSVRDETAGVRIFGIDPLSEANTPYQLGMISGEFLAPDDRQGVLVGQPLAEKL
                     GLNVGDKFSLSANTSNGDVAEQVFTIRGIYSTETNAFDSTTVFLPLAKAQTITQAENH
                     ASTIFVLLNDKAQTEPVVAALKTSKYKVLTWTEMNALIIQYEEFANAYMVILYLIVLA
                     ITATVIVNTLVMAVFERTREIGILSAIGMKGGRIMAMFLAESTLLAVGGIIMGLILGN
                     LVVAYFTKNGFYIGNMGITGLLIGNTIYTELTLEDTINLTVIALVITLLAGLYPALLA
                     ARMEPVEALHGGQ"
     gene            complement(2008..>2310)
                     /locus_tag="AC16CB_07525"
     CDS             complement(2008..>2310)
                     /locus_tag="AC16CB_07525"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR3342356.1"
                     /translation="RQISLLFVLEGTMMGVVGIAAGIVIGLAINGLLRQVGLDYSQFA
                     NVTSYMALVGGRVYPEWGLDKIVGRGLTVAIISALAAFIPAREAAQREPAEALHYV"
BASE COUNT          526 a          652 c          631 g          501 t
ORIGIN      
        1 tttcaactca cgcaccaagg ctgatctcct ccgcctcaga gttctcaaca atgaccccat
       61 cgcgcagcgt gatcacacgc cgggcgtatt ggatcacacg cgggtcatgc gtcgcaaaaa
      121 caaaagtaac gccggtctcc tgattaagcc ttgtcatcgt ttccatgacc tgtttgccat
      181 taggcgtatc caggttggcg gtgggttcat ctgccagaac cagcgttggt tgggttgcca
      241 gggcgcgagc aacagccacg cgctgctgct ccccgccgct caactgatca gggcgatgga
      301 aagcgcgctc ggtaaggtca accgattcga gcagctgcat gacacgggcg cgacgctcgg
      361 agctgctgtg gccgtttaag agcaatggca gctctacgtt ttcataagcc gtcagcgtcg
      421 ggattagggc aaagaactga aagataaaac cgatcgtacc gcggcgcatg ttggcgcgct
      481 gattgcgatt gagctggctg acatccttgc catccacgta gacgcgcccg ctggttggct
      541 tgtccaggca cccaatcaac tgcaagaggg tggtcttccc cgaaccagac gggccaacca
      601 gagcagcaaa ttcaccactc tcaatggaaa gactgacgcc gcgcagcgcg tgggtttcaa
      661 ccttgccagt cttatagatc cgcgtgacat tttcgattct tgcgacttcc attttatact
      721 ccttagaagt tacgcagttg gggaggtgat gtccccttca actacgtaag tcctgctccc
      781 attattgtcc gccgtgcagg gcttcgacgg gctccatgcg cgcagcgagc aaggccggat
      841 aaagcccggc cagaagtgtg atgacaagcg ctatgacggt caaattgatc gtatcttcca
      901 gggtcagctc cgtgtaaatg gtgttgccga tgagcaagcc ggtaataccc atattgccga
      961 tgtaaaatcc atttttcgtg aaataggcaa ccaccagatt gcccaggatc aagcccatga
     1021 tgatgccgcc cacggctagc agcgtagatt cagccaggaa catcgccatg atgcggcctc
     1081 ccttcatgcc aatcgccgaa aggatgccga tctcacgcgt gcgctcgaag accgccatga
     1141 cgagcgtatt cacaatcacg gtggctgtta tggccagcac aatcaggtag agaatgacca
     1201 tataggcatt ggcaaattct tcgtactgga tgatgagcgc attcatctct gtccaggtca
     1261 agaccttata ttttgaggtt ttcagcgcgg cgacgacggg ttcggtttgt gccttgtcat
     1321 tcaacaagac gaagatcgtg ctggcgtggt tctctgcctg ggtgatggtc tgcgctttgg
     1381 caagtggcag gaatacggtg gtgctgtcga aggcattggt ctccgtggag tagatgcccc
     1441 gtatggtaaa aacctgttca gccacatcgc cgttcgaggt atttgccgag aggctgaact
     1501 tgtcaccaac atttaatccc agtttttccg ccagcggttg ccctacgagc acgccttggc
     1561 gatcatcggg tgccagaaat tcaccactga tcattcctaa ctggtaaggt gtattggctt
     1621 cagagagcgg gtcaatgccg aagattcgca cccctgctgt ttcatcgcgg acagaaacga
     1681 tcccgctagc aaaaagcctg ggtgtggcta ctttcaccgg agccagcgag gcgagctgct
     1741 cagcaacttg agctgggttt tcgatcaggt cctcccactt gaggctggtc ttgctctcat
     1801 cgtaagattg cgcccgtatc tgcagatgac cgctttgcag ccggatggtt gcatccatag
     1861 cgctgcccat ctcaccctgg atgaaggccg ccatcagcag cagcagagcc aggccgagag
     1921 ccagcgccag ggatgagaag aatgaacggc gacggttgcg cccgaggtcg cggaaagcca
     1981 ttttaaagag ttgtatcatt gccaacctca cacgtaatgc agagcttcag cgggctcacg
     2041 ttgggccgcc tcgcgcgccg ggatgaaggc agccagcgcc gagatgatcg ccacggttag
     2101 ccctcgaccg acaatcttat caaggcccca ctcaggatat acgcgcccgc cgaccaacgc
     2161 catgtaactg gtgacattgg cgaactggct gtaatcaagt ccaacctgcc tgaggaggcc
     2221 attaatggca aggccaatga caatcccagc cgctatgccg accactccca tcatcgtgcc
     2281 ttccaagaca aacagaagag aaatctggcg
//
LOCUS       DBNGSX010000247         8482 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_573746, whole genome shotgun sequence.
ACCESSION   DBNGSX010000247 DBNGSX010000000
VERSION     DBNGSX010000247.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8482)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8482)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8482
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_573746"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..353)
                     /locus_tag="AC16CB_07530"
     CDS             complement(165..353)
                     /locus_tag="AC16CB_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342357.1"
                     /translation="MSYVSTQNRGYVFAAFLGAAFGGVFVMLATKAIPKMMSQMMAGM
                     MQNMMSQMSAEGCNPAEM"
     gene            complement(441..1274)
                     /locus_tag="AC16CB_07535"
     CDS             complement(441..1274)
                     /locus_tag="AC16CB_07535"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR3342358.1"
                     /translation="MYAKKQNRVRPRVIGCFATLLAILAIPGLYYGIQAYLRSKPITP
                     TQYIAHRGGPIHAPENTLSAFRTAIQQGVDWLEFDVQMTKDDELVVIHDESVDRTTNG
                     MGEVRNLTLDQIRALDAGSGEQVPTFEEVVRLAKMNGVKILPETKSAHLYPGIETKLM
                     QELEELDYVDQTVVQSFEPASLDTLHRLNPDLNFCALYGLWQFDISNPPADSQFVCPM
                     AEMVLIFPSMIQQAHREGRQVFVWFGVLEHPLMFRAMRFFGADGLMSDDPLLLVQSVA
                     P"
     gene            complement(1375..2067)
                     /locus_tag="AC16CB_07540"
     CDS             complement(1375..2067)
                     /locus_tag="AC16CB_07540"
                     /inference="COORDINATES: protein motif:HMM:NF015534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HIR3342359.1"
                     /translation="MHKGYILLEGGSEFGGKMAEPDLRAIELAGGLNAPIAIIPAAAA
                     PDHNHQRAGLNGVRWFNRLGANSVSLLPLIDQVSADEDSITTALNSSSLIYLLGGFPE
                     YLGNTLANSMSWQAILSAYHAGAVIAGSSAGAMVLCQYYYSPETNKIADGLNLIPNAC
                     IIPHHNSFGKGWASRLQALLPGVILIGIDEQTGMIDDGLDGEWNVYGKGAITLYKNGE
                     VIIYGAGEPLML"
     gene            complement(2334..2792)
                     /locus_tag="AC16CB_07545"
     CDS             complement(2334..2792)
                     /locus_tag="AC16CB_07545"
                     /inference="COORDINATES: protein motif:HMM:NF012481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR3342360.1"
                     /translation="MRSTIVYFSKFGNTQKVAEAIASVLEASGTVRVISTNQLSASEL
                     DESDLIIMGSPTHRMNLPEVVRPVLESLPRRILRGKLTAAFDTSYKMSMWLVPFTAAP
                     KLAQKLRRLGGKRVIHPETFHVVGKDGPLYDGELERAKKWTALILQRAEA"
     gene            complement(2842..3441)
                     /locus_tag="AC16CB_07550"
     CDS             complement(2842..3441)
                     /locus_tag="AC16CB_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3342361.1"
                     /translation="MVRYKKEEREQFLSRTRQLLLDSAAEEFAHKGYAEANINHISKS
                     AGFAKGTIYNYFASKEELFLDLLVTTAEQHIEEIIAYVNQEDDPRRRLERFFEAGFAF
                     VSQNLARTRVLINTTYGPNPDFKLQLYQAYQSLFQYVSRQIVALGIDEGIFREVEPNS
                     MGTLLMTIYLGTASQVSEDGQIWLDPKQVADFAWHALRK"
     gene            complement(3696..3869)
                     /locus_tag="AC16CB_07555"
     CDS             complement(3696..3869)
                     /locus_tag="AC16CB_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342362.1"
                     /translation="MQKYQGMINLVLKAVALAMAVASLVMGILGTSTVEAIQTLLSIG
                     LFSLALAALQRTE"
     gene            complement(3988..4407)
                     /locus_tag="AC16CB_07560"
     CDS             complement(3988..4407)
                     /locus_tag="AC16CB_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342363.1"
                     /translation="MVHALEESRRVLTHNGRLIDIRPLSSYPPVEVITSNGARLAGRA
                     DDSLGIPDDQAANNAIADAVRQGIFFKDADERFEFFYYWDTMEQMQAYIKDRWDDWVI
                     LRDTTLQNAAQIISESTGQIQLRITLTMLISVYGKRG"
     gene            complement(4426..4728)
                     /locus_tag="AC16CB_07565"
     CDS             complement(4426..4728)
                     /locus_tag="AC16CB_07565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3342364.1"
                     /translation="MSVYVLRDPEESEIQYLDRLGNLKGMRVLEIGSGDGRMTKRYAA
                     MARSVVGIDPDLESLAIAAKSQPDSSLSDVNLALALAQALPFPDEHFDRVIMAWSL"
     gene            complement(4817..7429)
                     /locus_tag="AC16CB_07570"
     CDS             complement(4817..7429)
                     /locus_tag="AC16CB_07570"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HIR3342365.1"
                     /translation="MLHESLAKDTKSGLLPTPSLPEDMGSVDLSENARTVLIRRYVRR
                     GRDGKPAESIEEMFWRVAYHVAKVEEAWDQDFMSRAGQYYDLLTSKRFFPNSPTFTGA
                     GTPLGQLAACFVLPITDDMGRDTAGIFQSLRDAALIQQTGGGNGFGFSRLRPKGTMVK
                     SSAGQATGPVGFLRVYDKAFGEIAQGGSRRGANMAVLRVDHPDIEEFITCKTDENAIT
                     NFNISVGVTDDFMRAVHNDEPWQLRFPDATAPEYHGFHGTLNEADASGVPIKTIKTVR
                     AREIFEKIVRQAHHNGEPGVLFLDAANRSNPVPHLYELESTNPCGEQFLGPYENCCLG
                     SINLAQHLGPEGTVDWEKLRESVVLSTIFLDDVVQANAYVPAVPQLKEAAHRARRIGL
                     GIMGLGDLMYHAGIRYGSQEGQEFGAQVMEFVRYHAMLTSIELAQQRGPFPAIEGSIY
                     DPQNLVWQPPTPLVAYKGDWGRPALDWAKVVDGIRMHGIRNAAQTTVAPTGTIATVAG
                     CESYGCEPVFALAYIRHVNDHGKDLQLTYTSPLFEKALLEAGLDEKRRQEIVERIMEL
                     GTCQGIDDLPDHIRNIFVVSSDITADEHVRMQAALQAFVDNSLSKTINFPPDATEEDV
                     ATAYKLAWDLGCKGITVYVTGSRQKVVLETKATLKQKQISEDKDSQPTQTSQVPMWQD
                     KKKPRPRSLRGYTYSISTPLGKAFVTINVNGEEKPFEVFINTAKAGSDTAAVSEAIGR
                     LISYILRLSSPVDARERLTEVWRQLSGIGGGRSLGFGPNRVRSLPDGVAQLLAEYLEN
                     SQNDEHGGSAESEGGNGFRYMSESARSVSQEEMPMEQGHLFKVGDLCPECGQAAVVNE
                     EGCRKCYSCGFSEC"
     gene            complement(7509..7958)
                     /gene="nrdR"
                     /locus_tag="AC16CB_07575"
     CDS             complement(7509..7958)
                     /gene="nrdR"
                     /locus_tag="AC16CB_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIR3342366.1"
                     /translation="MRCPYCKHEQSRVVDTSHDTRGGVRRRRQCENCGQRFSTYERPI
                     LATPMVVKQDGTREEFDREKLIRGIRISCAKRPVSANDIERLVGEVESKLQAMERSEV
                     SSRIVGDMVIAGLKELDQIAYIRYAIVYLGLDDLHSIRKEIDRLLEG"
BASE COUNT         2163 a         2267 c         1933 g         2119 t
ORIGIN      
        1 taacccaatg accattgacg ctcgataaac acacccaccg aggtagccag ttgatgatta
       61 tagcatcacc taactgtatc cagattgtta ttccagctga tcgatgacta cccccccggt
      121 acagacactt ctgggacttc gacgaagccc gccatcatac gttgtcacat ctcggctgga
      181 ttacaacctt ctgcactcat ttgagacatc atattttgca tcatgcctgc catcatttgc
      241 gacatcatct tgggaatcgc tttcgtggcg agcataacga aaacaccacc aaatgctgct
      301 cccaaaaatg ctgcaaacac ataaccccta ttctgagtac ttacataaga catggtatta
      361 gctcctactg gattgaaaat cactaacttt taccattttt ccctagacaa ggaatagcta
      421 cattctacaa gctgaatttc ctaaggcgcg acggattgca ccaatagcag cggatcatca
      481 ctcatcaaac catccgcacc gaaaaagcgc atagcgcgaa acatcaatgg gtgttccagt
      541 acaccaaacc agacgaaaac ctgacgtccc tctctatgag cctgctgaat catcgacgga
      601 aatatcaaga ccatttcagc catggggcaa acaaactggg aatcggcagg tggattgctg
      661 atgtcaaatt gccagagacc atagagagca cagaaattta ggtcggggtt taatcgatgc
      721 agtgtatcta gcgaggcagg ttcaaatgat tgcacaacag tctggtcaac ataatctagc
      781 tcttccaatt cttgcattaa tttcgtttca atgcctggat agaggtgtgc tgactttgtc
      841 tctggaagta ttttcacacc gttcattttc gctagcctga ccacttcctc aaaagtgggc
      901 acttgttctc cactgcctgc atctagtgca cgtatttgat ccagggtgag attcctcact
      961 tcacccattc cattggttgt gcggtccacg ctctcatcgt ggattacaac cagttcatca
     1021 tctttcgtca tttgcacatc aaattcaagc cagtcaacgc cctgttgtat ggctgtacgg
     1081 aaggcagaca gcgtgttttc gggtgcgtgt attgggcctc ctcgatgagc gatgtactgt
     1141 gtcggcgtga ttggcttgct acgcaaatat gcttgaatac catagtacaa cccaggaatt
     1201 gcaagaatgg caaggagggt cgcaaaacat cctataactc ggggacgaac acgattttgt
     1261 tttttggcat acattaccgc ctcctcagta ttattggtgt tatcccctcc tcaattgtac
     1321 acattagcga caatttctat agaccgattc gctatataca tcacgctagt tcacctaaag
     1381 cattagcggt tctcccgcgc catatataat gacttcgcca ttcttgtata acgtgatagc
     1441 ccccttacca tacacattcc attccccatc caagccatca tcaatcattc ctgtctgctc
     1501 atcgatccca ataaggatta ctccaggtaa taaagcttga agacgcgagg cccatccctt
     1561 accaaagcta ttatgatgcg ggattataca agcatttggg atcaaattca atccatcagc
     1621 aatcttattt gtttctgggc tgtaatagta ctggcacagg accattgctc ccgcactgct
     1681 acctgcaatg accgctccag cgtgataggc tgacaggatc gcctgccagc tcatgctgtt
     1741 cgctagcgta ttcccaaggt attcgggaaa accacccagc aggtagatga ggctcgaact
     1801 atttagcgcc gttgtgatcg aatcttcatc ggcagaaacc tgatcgatca agggcaaaag
     1861 ggaaacactg tttgcaccca agcgattgaa ccatcgcaca ccgttgagac ctgcgcgttg
     1921 gtggttatgg tcgggtgctg cagcagcagg gatgatagcg ataggtgcat tcaatccgcc
     1981 agcaagctca atagcccgta aatcgggttc agccattttt ccaccaaatt cagatccgcc
     2041 ttctaagagg atataccctt tgtgcatttc acaaccctat tctgaccagc tccaaactga
     2101 ataatgctgt agacaaacgc gtgtcatgac ataagcatcg aatgaacaat cctgcaactg
     2161 cacaggtgag ccacagagct caaaaagttg tatgcaactc tattttatta tgaaaatatt
     2221 tgaatgtatg aaaagggtat atttgaattt accttaatgg tcgccagctg gcctttcgtc
     2281 cgttgtgctc cagcgtgttc aacgtcaagc ataggtttat ttagtcagat tttctacgct
     2341 tctgcacgct gcaggattaa cgcagtccat tttttcgccc tctcgagctc accatcgtac
     2401 aatgggccat ctttcccgac cacgtgaaag gtttccggat ggatgacgcg ttttcctccc
     2461 agtctgcgaa gtttttgagc cagttttggt gccgcggtga aagggactaa ccacatcgac
     2521 attttgtagg atgtgtcaaa agcggctgtt agcttgcctc gcaagattcg ccggggcagc
     2581 gattcaagaa ctgggcgaac cacctcgggt agattcatcc ggtgcgttgg gcttcccatg
     2641 atgatcaaat ccgactcatc gagctcagag gctgagagtt ggtttgtaga tatcacccga
     2701 acagttccac tggcttccag cacgctcgca atcgcctctg cgactttttg ggtattgcca
     2761 aatttagaaa aatatacaat cgttgatctc attagtattc ctccaatcaa tttacgaaat
     2821 attgccagct tcctggacgt tctattttcg caaggcgtgc caagcgaaat cagcaacctg
     2881 cttggggtcc agccagatct ggccatcttc gctaacctgt gaggctgtgc ccaggtagat
     2941 ggtcatcaac agtgtaccca tggagtttgg ttccacctcc cgaaagattc cctcatcaat
     3001 ccctaaggca acgatttgtc tactgacata ctgaaaaagt gactggtaag cctgatagag
     3061 ctgcagctta aaatctgggt ttggtccata ggtggtgttg atcaggaccc gtgttcgtgc
     3121 gagattctgc gacacgaatg caaagcctgc ttcgaaaaat cgctccagcc gacggcgggg
     3181 gtcgtcttct tgatttacat aagcaatgat ttcttcaatg tgctgctcag ctgtcgtcac
     3241 tagcaggtcc aagaagagtt cttctttgct agcaaagtaa ttgtatatag tgcccttggc
     3301 gaagcccgct gacttggata tatggttgat atttgcctcc gcataaccct tgtgggcaaa
     3361 ttcttcggct gccgagtcta ataacaattg gcgggtcctg ctcaaaaact gctctcgttc
     3421 ttcttttttg tagcgcacca tcgaaacctc tctttatatg ggtgggcagt cacccacatt
     3481 attgcacaaa taacgcggtc tgtcaagata attaatcgat tgttacactg ttttgtgtag
     3541 gaaacggatg gtaaatttca cccctaagcg aaaaaaatcc cagctgggcc aatggcgtag
     3601 ccaaccaggt tgctcttgca cagtaaaacc aggcaagcgt tctcgaggca cccccccact
     3661 acccccacta attctttggc aacagcgtat ccagcctatt ccgtcctttg aagagccgcc
     3721 agcgcgaggc tgaacaaccc tatgcttagc aaggtttgga tagcctcaac cgtgcttgtc
     3781 cctaatatac ccatgacaag actggctacc gccatggcta gcgcaactgc tttcaaaact
     3841 agattgatca ttccctgata tttctgcata tctcctcctc cttgtattag gaattcaaaa
     3901 tgaaattatt caagagtatt tcaaaaaggt taaagttgta aattgctatc gcatttaaca
     3961 aatagcgaac ctctatccgt ttgagattta accccgctta ccgtaaacag agataagcat
     4021 ggtaagagtg attcgtaatt gtatttgacc agtgctttca ctgattatct gggctgcgtt
     4081 ctgaagagtt gtgtctcgca agataaccca atcatcccat ctatccttta tgtaggcttg
     4141 catttgctcc atagtatccc aataataaaa aaattcaaaa cgttcatcgg catccttgaa
     4201 gaaaatgccc tgtcgaacgg catctgcaat ggcgttattg gctgcctgat cgtcaggtat
     4261 accaagggaa tcatcggctc taccagccaa cctagcccca ttgctggtaa tcacttcaac
     4321 gggcggatat gatgacagtg gacggatatc aatcaaacgc ccgttatgcg tcaatactcg
     4381 ccggctttcc tcaagggcat gaaccatgcc ctcatgcgct actcatcaaa gcgaccaggc
     4441 cataatcacg cggtcaaagt gctcatctgg aaacggcagc gcttgcgcta gagccagcgc
     4501 caggttgaca tctgagagtg aactgtccgg ctgtgacttc gcggcaattg ccaggctttc
     4561 cagatctgga tcgatgccca caacggatcg tgccatcgcg gcataacgct tagtcattcg
     4621 gccatcccca gaaccaattt ccagcacacg catccccttg agattgccca gccgatcgag
     4681 atattggatt tcactctctt caggatctct caagacataa acgctcatat gaactccaat
     4741 caaatgcttc ttttcaaagt agaaaaaaca ggcagggatt gttgctccct gcccactcct
     4801 ccatgattgg aaaattctag cattcgctaa acccgcacga atagcatttt ctgcatccct
     4861 cttcattcac caccgctgcc tgaccacact cgggacacaa atctcccact ttgaacaaat
     4921 gcccctgctc catgggcatt tcctcctggg aaaccgacct ggctgattcg ctcatatagc
     4981 ggaaaccatt gccaccctcg gattcagcgc tcccaccgtg ttcgtcgttt tgggagttct
     5041 ccaggtattc cgccagcaac tgtgcgacgc catcaggcaa tgaacgtacc cggttcgggc
     5101 caaaacccaa cgaacgcccg ccaccaatcc ccgaaagctg acgccaaacc tctgtcaggc
     5161 gctcacgagc gtctactgga gagctgaggc gcaggatata ggagatcagt ctaccaatcg
     5221 cctccgagac ggctgccgta tcggagcctg ccttggctgt gtttatgaag acctcaaagg
     5281 gcttttcttc accattcacg tttatggtga caaacgcctt gcccagcggg gtactgatgc
     5341 tgtaggtgta accacgcaag ctgcgagggc gtggcttttt cttatcttgc cacataggca
     5401 cctgggatgt ctgggtgggt tgagagtctt tgtcctcgct gatctgtttc tgtttgagcg
     5461 tggctttagt ctctagaaca accttctgtc ttgaaccggt cacatagact gttatgccct
     5521 tgcatcccaa atcccaagcc agcttgtagg ctgtagccac atcctcctcg gtcgcatcgg
     5581 gagggaaatt aatcgttttg ctcaggctat tgtcgacgaa tgcctgcagt gcggcctgca
     5641 tgcgtacatg ctcgtcggct gtgatatccg atgagaccac aaagatattg cggatatgat
     5701 ccggcaaatc atctataccc tggcaagtgc ctagctccat aattctttca acaatctcct
     5761 ggcggcgctt ctcatccagg ccggcttcaa ggagcgcttt ttcaaataga gggctggtat
     5821 aggtcaattg cagatctttg ccgtggtcgt tcacgtgacg gatataggcc agagcaaaga
     5881 caggctcgca accatagctt tcacagccag ctacagtggc aatggttccg gtgggagcga
     5941 cggtggtctg tgcagcgttg cggatgccat gcatgcggat gccatccacc actttagccc
     6001 aatcaagagc cgggcgaccc caatctccct tataggccac caatggggta ggaggttgcc
     6061 agacaagatt ctggggatcg taaatgctac cttcaatagc tgggaaggga ccacgctgct
     6121 gcgccagctc aatgctggtg agcattgcat gataacgcac gaattccatc acctgtgcgc
     6181 caaactcctg tccttcctgg gagccatagc ggataccggc atggtacatg agatcaccca
     6241 gacccatgat tcctaagcca atacgacgag cacggtgagc agcttctttc agttgaggca
     6301 cagccgggac atacgcgttg gcctggacaa catcatccag aaagatagtt gaaagcacca
     6361 cgctttcgcg caacttctcc caatctaccg tcccctctgg tccaaggtgc tgggctagat
     6421 ttatcgaccc caaacaacaa ttctcataag ggccaaggaa ttgttcaccg catggattgg
     6481 tcgattcaag ttcatagagg tgaggaaccg gatttgagcg gttggctgca tccaggaata
     6541 gcactccggg ctcgccgtta tggtgcgcct gacggacaat cttttcaaaa atttcacgcg
     6601 ccctgaccgt cttaatcgtc ttgatgggca caccggatgc atccgcctcg tttaacgtgc
     6661 cgtggaagcc atgatactcc ggagcggtgg catccggaaa gcgcaattgc caaggctcat
     6721 cattgtgcac cgcccgcatg aaatcgtcgg tcacgcccac cgagatattg aaattggtta
     6781 ttgcgttctc atcagtttta caggtgataa actcctcaat atccgggtgg tccacgcgca
     6841 gaactgccat attcgccccg cgtcggctgc ctccttgggc tatttcccca aaagccttat
     6901 cgtagaccct caagaagcct accggtccgg tagcctgccc agcagacgat ttgaccatcg
     6961 tccctttggg acgcagacgt gagaagccaa acccattacc gccgccagtc tgctgaatca
     7021 gggctgcatc gcgcaagctc tggaaaatgc ccgccgtgtc gcgccccata tcatcggtga
     7081 taggcagcac aaaacaggct gcaagctgac ccaggggagt accagcacca gtgaaagtgg
     7141 gtgaatttgg gaagaatcgc ttgctcgtaa gcaaatcata atactgacct gctcgtgaca
     7201 tgaaatcctg atcccacgct tcctccacct tagccacatg gtaggcaacc ctccaaaaca
     7261 tttcttcaat cgactcagcc ggttttccat cccggccgcg tcgcacgtaa cgccggatca
     7321 gcactgtgcg ggcattctca gataaatcaa cactacccat atcttcgggc aaagatgggg
     7381 tgggtaacaa accactcttg gtgtctttcg ctagggattc atgcaacatg ggcgcgtctc
     7441 ctaaaaacca gtatagattc cttgcaatac ggcagaatca ctcccctagt ctataggaga
     7501 gcgcaaaact agccctctaa aagccgatcg atttccttgc ggatagaatg caggtcatcc
     7561 agtccaaggt aaacgatggc gtagcgtatg taagcgattt gatctagctc tttcaaacca
     7621 gcaatcacca tgtcccccac gatacgagaa gagacttccg agcgctccat tgcctggagc
     7681 ttagactcaa cctctccaac aagtcgttca atgtcgttag cagagaccgg gcgcttggca
     7741 caagaaatgc gaatgccgcg gatcaacttc tcgcggtcaa attcctcccg cgtgccatcc
     7801 tgcttaacaa ccatgggtgt tgccagtata ggacgctcgt acgtactgaa gcgctgacca
     7861 caattttcac attgccgacg ccgtcgaaca ccgccacggg tatcgtggga tgtgtcaaca
     7921 actcgagact gttcatgctt gcaataggga cagcgcattt ggctccaaat aaatagaaca
     7981 gatgtaagcg ccacatgtgg gggtaatgct agctacgacc agcacatgtg gcgtatagcg
     8041 gtcaaaattc tagcacagac aagcccattg cgcaaggcta aaacggatag cttaacatta
     8101 aaatttcaat tttgtcggaa atttaatgtc gagaatgtct ctcgaagctt ctgaagagcc
     8161 aggggtgaga tgtgcgacgg tcgggtgtcg cacatctccc tgccacgaag actcatgcga
     8221 aggcaatcac aagccagaac gtggtctgac ggcaaaagat aatttcaggc cttctggttt
     8281 tagcaggaga tatcccagaa tatggtagca atcccgcaaa tctcggaaga cccctaatgt
     8341 atagacaacc aacaacgacc gggaggagga gtccggcagt tgttggctga aacactataa
     8401 atggtgaaac caggcatccg caattagcgg ccgcggtttc gcaagaaggt cggaatatcc
     8461 aggtcttcgg tgttgaaagc tc
//
LOCUS       DBNGSX010000248         3669 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_643873, whole genome shotgun sequence.
ACCESSION   DBNGSX010000248 DBNGSX010000000
VERSION     DBNGSX010000248.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_643873"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="AC16CB_07580"
     CDS             complement(<1..838)
                     /locus_tag="AC16CB_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3342367.1"
                     /translation="MESGIFHATYQSDMALRRTHAQKIRIAIFLIFMCVFPFFASRYT
                     LTLANQVGIATIGAIGLNILVGYTGQISLGQGGFLAVGAYTAGILTARLGMPWYVSTV
                     LACLVTALIGAIFGIPSLRLKGLYLAIATLAAQEIILWLVTHWDALTGGVDALVVPNP
                     TFFGLQLNTDFRFYFVIWGLAGLTALATINLFRSHYGRAFIAIRDQDIAAEVMGVNIF
                     RYKLLAFAISSFFVGLAGALTAHYRSIVTWERFTIDVSVLYLAMIIIGGLGSVPGSFF
                     GAT"
     gene            complement(1002..1874)
                     /locus_tag="AC16CB_07585"
     CDS             complement(1002..1874)
                     /locus_tag="AC16CB_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3342368.1"
                     /translation="MELFIQLTLTGLTNGAILALAALGFVLIYKSSDVINFAQGEFLL
                     VGAYITFAMIAQFGLVWPLGMILTMFLAVVMGVIVERLVLRPMIGEPIISIIMVTIGL
                     SSLLRAIVSGIWGNLPRAFPPFIPSQTVKILSAVVGADRLWAFVIALILLGLFSLFFR
                     RSKEGIAMRAVADDQQAALSMGISVKKIFAWAWSIAAATAAIGGALVANIVGVSGELS
                     GFGLRVFPVVILGGLDSIPGAIVGGIIIGLLEAYTGGYIGQGLNTVIPFVLLIFILMV
                     RPYGLFGQEIIERV"
     gene            complement(1901..>3669)
                     /locus_tag="AC16CB_07590"
     CDS             complement(1901..>3669)
                     /locus_tag="AC16CB_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3342369.1"
                     /translation="FCLGMVSLGLQPEEAIAIIGDNRPEWVIAELAAQSAGAKSIGLY
                     QDSGVKEIIYIILHADVSFIIVEDQEQVDKILEMWSELRGVRKVIYYDPKGLRNYTED
                     FLISFQDVEQLGQLYDRDHPGWFEERVAHGKASDIAILSTTSGTTGDPKLAALTHESM
                     VSMGHNLMKVDPMAPQDEFVSFLPLAWIGEQMMVISCGLKVGFTVNFPEEPETVQHDL
                     REIGPQVMFSPPRIWENLVSQVQVKIEDSSLLKKKLYNWAMPIGYQMADIRFRKETPS
                     LGLRWKYKLADWLIFQEIKDHLGLRRLKRAYTGGAALGPDVFRFFHALGVNLKQIYGQ
                     TEINGIAVVHRDGDIKFQTVGTPIPETEIKIDENGEILMRSPAVMTSYYRNPDATAGA
                     LKDGWLHSGDAGYFDEDGHLIVIDRAKDVMTLHDGTKFSPQFIENKLKFSPYVREAVV
                     FGGDWPFVSAMINIDFANVGKWAENHQIAYTTYTDLSQKVQVYELIRQQVERANADLP
                     PAARIQRFLLLHKELDADDAELTRTRKVRRNLIAQRYDDIISALYSQSSHLDVETTIT
                     YQDGRTAVLQTRLKIESMNGGG"
BASE COUNT         1024 a          987 c          825 g          833 t
ORIGIN      
        1 aggttgctcc aaagaacgag cctggtactg agcccaatcc accaataatg atcatagcca
       61 gataaagcac cgacacatca attgtaaatc gctcccaggt cacaatgcta cgataatggg
      121 ctgtgagtgc acctgccagg ccgacaaaga acgatgagat cgcaaatgcc aggagcttgt
      181 atcgaaagat gtttacgccc attacttcgg cagcaatatc ctggtcccgg atagcgataa
      241 acgcgcgtcc ataatgggag cggaataaat tgattgtcgc cagcgcggtt aggccagcca
      301 gaccccaaat gacaaaatag aaacggaagt ccgtgttcaa ctgtagacca aaaaaggtcg
      361 gatttggaac aaccagcgca tcgacacccc ctgtcagagc atcccaatga gtgaccagcc
      421 aaaggatgat ctcctgtgca gccagggtag caatcgccag atagaggcct ttcaggcgta
      481 gagatgggat gccaaatatt gctcctatta aagccgtcac caggcaagcc agaacggtcg
      541 atacgtacca aggcatgccc aaacgggcag tcaagatgcc agcagtgtat gctcctaccg
      601 ccaaaaatcc cccctgcccc aggctgatct gaccggtata tcctacgaga atgtttaacc
      661 ctatcgcacc aatcgtagcg attccgacct ggttcgccag cgtcagggta tagcgactcg
      721 caaagaaagg aaatacgcac ataaaaatca aaaaaatcgc aatacgtatt ttctgcgcgt
      781 gcgtgcgccg aagagccata tccgactggt atgtagcgtg aaaaatacct gattccatag
      841 ctcaaagact ccaataccaa agcgtttcac acaaagctaa tggttccgta actaaataat
      901 acataagtta cgcagttcga gaggtgatgg gggaatttgg gcgggctgcg cccgcccaaa
      961 ctccccctct tccctcttca actgcgtaag tcctataata gttaaacacg ttcgatgatc
     1021 tcctggccaa atagcccgta ggggcgcacc atcaggataa agataagcaa cacaaatgga
     1081 atcacagtat taagaccttg accaatatag ccgccagtgt atgcctccag caagccgata
     1141 atgatccctc ccactatagc acctggaatt gaatccaaac cgcccaagat cacgaccggg
     1201 aatacgcgca gaccaaaacc actgagttca ccgcttacac caacaatgtt ggcgactagc
     1261 gctcccccga tagctgccgt ggctgctgcg attgaccaag cccaggcaaa tattttttta
     1321 acactaattc ccatgctgag tgctgcttgc tgatcatcag caacagcccg catggcaatc
     1381 ccttcctttg aacgacggaa aaacagagag aacaaaccta acaagatcaa cgcgatgaca
     1441 aacgcccaca agcgatcagc acccaccaca gcggacagaa ttttgaccgt ttggctgggg
     1501 ataaacggtg gaaatgccct ggggaggtta ccccaaatgc cgctgacaat agccctcagc
     1561 aagctgctta atccaatcgt caccatgata atggagatga ttggttcacc gatcatgggg
     1621 cgcaaaacca aacgctccac aatgaccccc ataactaccg ctagaaacat ggttaagatc
     1681 atgcctaatg gccataccag gccgaattgg gcaatcatgg caaatgtgat gtacgcgccc
     1741 accagcagga attcaccctg ggcgaaattg atcacatcgc tggatttata gatcagcaca
     1801 aatcccaaag cggccaaagc caggatggca ccattcgtca aaccagtcag agttagttgg
     1861 ataaagagtt ccataatatt tccgttgatg cggtcacccg ttacccgcca ccattcatgc
     1921 tctcgatctt caggcgagtc tgcaagacag ctgtgcgacc gtcttgatac gttatcgtcg
     1981 tttccacatc caggtgcgag gattggctgt acaatgcgga gatgatatcg tcatagcgct
     2041 gggcaatgag attccgtctt acttttcggg tacgagtaag ctcggcatca tcagcatcta
     2101 attctttatg aagcaagaga aaacgctgga tacgagcagc cggcggcaaa tctgcgttgg
     2161 cgcgctctac ctgctggcgg atgagttcgt acacctggac tttttgtgag agatccgtgt
     2221 atgtggtata agcgatctga tgattttcag cccacttgcc tacgtttgca aaatcaatat
     2281 tgatcattgc gctgacaaaa ggccagtcgc ctccgaatac aaccgcctct cggacataag
     2341 ggctgaactt aagtttattc tcaatgaatt gggggctgaa ctttgtgcca tcatgtagag
     2401 tcattacgtc tttggcacga tcgatgacaa tcaggtgacc atcttcatca aaatagcctg
     2461 cgtctccaga atgcaaccag ccatccttca gcgcacctgc ggtcgcatcg ggattgcgat
     2521 agtagctagt catcacagct ggagaacgca tcaatatttc gccattttca tcgattttga
     2581 tctcggtttc ggggatcgga gtgccaacag tctggaattt gatgtcacca tcgcggtgga
     2641 caaccgcaat gccgttgatc tccgtttgcc cgtaaatttg ttttaaattc acgcccaggg
     2701 cgtgaaaaaa gcgaaataca tcggggccaa gcgccgcccc acccgtataa gcgcgtttca
     2761 agcgacgcaa acccagatga tccttaattt cttgaaatat caaccaatct gcaagcttgt
     2821 atttccaccg caaccccaga gacggcgtct ctttacgaaa acgaatgtcc gccatctgat
     2881 aaccgattgg catcgcccag ttgtacagtt ttttctttag taaagagcta tcttcaatct
     2941 ttacttgtac ctgggaaacc aaattctccc aaatgcgtgg tgggctgaac atgacctggg
     3001 gaccaatttc gcgcagatca tgttgcactg tctccggctc ttcagggaaa ttgaccgtga
     3061 aacccacttt caagccgcaa gagatgacca tcatctgctc gccgatccaa gccagaggca
     3121 gaaaagagac gaactcatcc tgaggcgcca tggggtccac tttcattagg ttgtgcccca
     3181 tgctgaccat gctctcatgg gttagtgcag ccagctttgg atctccagtt gtccctgatg
     3241 tcgtagataa gattgctata tcactggctt ttccatgagc gactcgctct tcaaaccagc
     3301 cggggtggtc tctatcgtaa agctgaccca attgttcaac atcctgaaag gagatcagga
     3361 aatcttcggt atagttgcgc aatcccttcg ggtcataata aataactttg cgcacacccc
     3421 gcaactcaga ccacatttcc agaattttgt ctacctgctc ctgatcttct acaatgatga
     3481 atgacacatc agcatgcaaa atgatgtaaa tgatctcttt aacacccgaa tcttgataca
     3541 ggccgattga tttcgccccc gcagactgtg ctgccagttc agcaataacc cactccgggc
     3601 ggttatcccc aataatggca atcgcctcct cgggctgcag gcccaaacta accattccaa
     3661 gacagaagt
//
LOCUS       DBNGSX010000249         1867 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1197490, whole genome shotgun sequence.
ACCESSION   DBNGSX010000249 DBNGSX010000000
VERSION     DBNGSX010000249.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1197490"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..952
                     /locus_tag="AC16CB_07595"
     CDS             185..952
                     /locus_tag="AC16CB_07595"
                     /inference="COORDINATES: protein motif:HMM:NF016873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HIR3342370.1"
                     /translation="MELKELLVVIPHSGIVIPGEIPMANLSERFPVLVQNVDWYTNWL
                     YDFRDLFENRHIVFPYCSLILEANRHPDILDDSVPLKDVRGESVYKTQMAPNKELRET
                     LAKKYLIAFHKGIEACIESGVEFLLDGHSTVTARGVADNQIDLMNFQHSHLDSGPRYY
                     SPDVYVEAYATELQKRLPEAKVTVNSSEYYTVYGYVCAEHSVKAMGRVGKRVPPIIQE
                     TNERLYKNPDRTPNVVAINRLRRAFAESIHAAYRKLH"
     gene            972..1499
                     /locus_tag="AC16CB_07600"
     CDS             972..1499
                     /locus_tag="AC16CB_07600"
                     /inference="COORDINATES: protein motif:HMM:NF015377.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HIR3342371.1"
                     /translation="MINLHITRQTFDFDCGAKALQTVMAYYGVEIRQDELMKELGTGR
                     DGTRVDKMISVSESKGFRVEAKEHWSIGEVKCYIDKRHPVIVLLQAWAERYMTLKDWR
                     ANHDDGHYAVIIGYTKDVLVFEDPASFRRTWLRENEFLARWHDQDPERNRKYQRFGMV
                     LLGKEPVPQTAEHMD"
BASE COUNT          521 a          426 c          458 g          462 t
ORIGIN      
        1 atacttaata ctcacacgaa ctttcttaca gaaaacgcct taattttaga ataccgttat
       61 tattgttaaa cgtgatgtca tttaacaata aatgcgaaat aattctcgaa taacagtaaa
      121 cgggaaatat ttttcgctac tcactagttc gggtgcgtga ttcatttcaa cgagagggac
      181 ttaaatggaa ctgaaagagc tgcttgtcgt tataccgcac agtggcattg ttattcctgg
      241 tgaaatccct atggcgaatc tttcagagcg attccctgta ctagtacaga atgtcgattg
      301 gtacacgaat tggctatacg atttccgaga tttgtttgaa aaccgacata tcgtcttccc
      361 ctactgcagc ctcatccttg aagctaatcg gcatcctgat attttggatg acagtgtacc
      421 cttgaaagac gttcgtggcg aatccgtcta caagacacaa atggcgccca ataaggaact
      481 gcgggaaacg ctcgccaaga aatatttgat cgccttccat aagggcatag aggcgtgtat
      541 cgaatccggg gtggagtttc tgctggacgg tcattccacg gtaacagctc ggggggtcgc
      601 agacaaccag attgacctga tgaatttcca gcattcccac ttggacagcg ggccgaggta
      661 ctattccccc gatgtctacg tcgaagccta tgcgacggag cttcaaaaga ggcttccgga
      721 ggccaaggtg actgtaaaca gttccgagta ttataccgta tacggctacg tgtgcgcgga
      781 acactcagtg aaagccatgg gtcgggtcgg caagcgggtg ccgccaatca ttcaggaaac
      841 caacgagcgc ctctacaaga atccggatcg aactccaaac gtggtggcga tcaacaggtt
      901 gcgccgcgct tttgcagagt ccattcatgc ggcttatcgg aaacttcact gattcagaga
      961 ggatggggta catgatcaac ttgcatatta ctagacaaac gttcgatttt gactgtgggg
     1021 ctaaagccct gcaaacagtc atggcctatt atggggtgga gattcgccag gatgaactga
     1081 tgaaggaact gggaacgggc agggacggca cccgcgtaga taaaatgata tccgtttctg
     1141 aatccaaggg gttccgggtt gaagcgaagg agcactggtc catcggagag gtgaagtgct
     1201 acattgacaa gaggcatccg gtgatcgttc tgctacaggc atgggcggaa agatacatga
     1261 cccttaaaga ttggcgggca aaccacgacg acggacatta cgcggtcatc attggttata
     1321 cgaaggacgt tttagttttc gaagatcctg catcgtttcg aagaacttgg ctcagggaaa
     1381 atgagttcct ggctcgatgg cacgaccaag atccagaaag aaatcgcaag tatcaacgat
     1441 tcggcatggt tctgctggga aaggagcctg tgccgcaaac cgcagagcat atggattgaa
     1501 agcttccgag agtctaagaa taggcagcag acatcgcacc atgttctatg tactccttgt
     1561 ctgaggtacg caaccgaacc ctgctgctgc aagggacgcg caaaaaatcc gcgcgcccct
     1621 gagcagcccg ttatcctaat cataacaatg tagccatgtg attctcaata ataccatatg
     1681 gtatttttcc atatcttttt gagcataatc gcaataccat atattgcggt tctcccgttt
     1741 taggtaaaat ctgacatatc tccaacatat cagcactttt gaatcactga aaaagctgtg
     1801 atactcagat catatctgca attttggcaa attacggata ttctcaatat agtgcgccac
     1861 gagcgca
//
LOCUS       DBNGSX010000250         8580 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1335906, whole genome shotgun sequence.
ACCESSION   DBNGSX010000250 DBNGSX010000000
VERSION     DBNGSX010000250.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8580)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8580)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8580
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1335906"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="AC16CB_07605"
     CDS             complement(<1..808)
                     /locus_tag="AC16CB_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR3342372.1"
                     /translation="MFLFVGSILSVAGYLYLKRNFDPDAGLSSSTQVSVEAVYTAAAQ
                     TLEAKLTETAAAQPTAFPTNTPIPPTETPAETSTPEFTPTSFFRPEPTGPMITVQIGS
                     NCRNGPSTAYDPPITTLAEGQKAEIHGRNSNSSWWYVQIPGRPGQYCWIFGNLVSVQG
                     EVSAVPVIAPPPIPVTATFTPKPDVRFDPSFNNIHECGGEPFAIFEIDNIGKEDFESM
                     SLKIEDTDDDEEIYSASSDNPFLSAPSECPEGDDELKDGDVAWIGGDVDDG"
     gene            complement(1078..1334)
                     /locus_tag="AC16CB_07610"
                     /pseudo
     CDS             complement(1078..1334)
                     /locus_tag="AC16CB_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561162.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            1544..1714
                     /locus_tag="AC16CB_07615"
     CDS             1544..1714
                     /locus_tag="AC16CB_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342373.1"
                     /translation="MEKPRLVETGSSSFFGEYLYDQVVPQGHFLTSSEENHRMGAVHK
                     QANQIVQKRRSG"
     gene            complement(2166..2903)
                     /locus_tag="AC16CB_07620"
     CDS             complement(2166..2903)
                     /locus_tag="AC16CB_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016875428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3342374.1"
                     /translation="MPYANNQGVHIHYQVIGDGPPLVLQHGFSASLMDWYDFGYVEEL
                     CNDYRLILVDARGHGESEKLYEPEQYDLEQFVSDVTTVLDDLHIKKTNYLGYSMGGSI
                     GFGMAKYARHRLNALIIGGAQPYGIKFEPLRELLSTGLETWVAIVETWGIYSTDGLAR
                     MRNNDARALLAVIQDRPDISDVLPGMTMPCLLYAGSEDDQYELIARCAEELSNVTLVS
                     LPGLNHFNIIPRSDLVVPYVKEFLAKP"
     gene            complement(3103..4107)
                     /locus_tag="AC16CB_07625"
     CDS             complement(3103..4107)
                     /locus_tag="AC16CB_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR3342375.1"
                     /translation="MESGKNFTIQDIANRANVSISTVSRVLNNTAPVAEETRQRVLSI
                     ITELGYKPNLFAQGLAGGQSRTIGVLTQLIGSPFYDVILRGILRGIDGSGYSPLFADG
                     GWDAEKDQIALYMFMQRQVDGLIVLNGHSPDEFLIEIALRIPMIIVGREIAELSEQCL
                     PFDDLEAAYKATQHLIDAGHRRIAHITGLPNHQDANERRDGYIKALEQNGIQPDPELI
                     IEGDFTEPSGVMAVEMLLMRGRIFSAIFAANDQLAYGARLALYRRGLRVPEDVSIIGF
                     DDQAPSAYMVPPLTSIRRPPLEIGEAAGRALIYLIQGKSISLPKFQSALVIRESVSRR
                     "
     gene            4495..5838
                     /locus_tag="AC16CB_07630"
     CDS             4495..5838
                     /locus_tag="AC16CB_07630"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3342376.1"
                     /translation="MRKNLLAIIILLVSLSMVLSACASQATPSPEAEVEAPPEETTEE
                     VTLRVLVHQNPPMVEFIEAFNAKFMEKYPNVTVDMSVVNANDLATVTQTRLTANDVDV
                     VDMFAFTNAAQPYMKNVEPPNWQTLIEAGYLLDISGQPFLDNYFPASIADAGTYNGKV
                     YQINLGTVSYSGIYYNESLFAEYNVAVPTTWDELVAACETFTAADIPCMTAGGKDGWP
                     IFVGAYGLIGSIYPDQAALVEGLWTGSIKWNDAKSMEMWDKMKVYAQNMMEAGASGIA
                     GDAAPGRFASGAVAMFSGGTWYAAAIEEAEPDFEWGYIPFPGSDNPEDNKYLFGKYDQ
                     GWVIAANSPNTDTGLLYLEEFSDPANYQEFVNAVGFFPTQPGATLDTQIGQEVAPYLE
                     NFRVGYEQYWVAPKGAGQYANPWASYFQPFGEFDDAQELADKVQADLQSGLDASQ"
     gene            5918..6847
                     /locus_tag="AC16CB_07635"
     CDS             5918..6847
                     /locus_tag="AC16CB_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018885081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3342377.1"
                     /translation="MMAYHFGRGRARLSPYLLLIPGILLYLLIALGPSLATSVFSFTD
                     ATGIRGAPIHWIGLENYREFLLLGQASRDNYAALGRTLIFCFFVTTIQFGLGLLVAML
                     VNQRLKGTNFFRTLYFMPVILGAVIQGLIWSLFLYPLGGPMARILEFFGTSSEFFGGQ
                     PNEAFAYVIVVQIWANMGTTMVIFLAGLQTIPDELYEAAQIDGASGWQVFSNVTWPLL
                     TPSVNTNLLLNIIGSLQAWQLFLVLIGYRNGTQVLGYLVYAQGFGQTSGSMVNPFRQG
                     YAAAASIVLFFLVLVLGMVTQTFLRRREERILG"
     gene            6844..7680
                     /locus_tag="AC16CB_07640"
     CDS             6844..7680
                     /locus_tag="AC16CB_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3342378.1"
                     /translation="MNTTMKTRPPINWGRVASYAALVLFAVIYAGPLLMLVNTSLKTL
                     PAFMKDATSLVTSLHFDNFIEAWEKANFPRYLTNSLIYTLSATLIYVVTAIFVAFPVA
                     RGYVRGANFILTMFVIALFLPPALIPQFQLMLRLGLYNNPVGYILLFTINPIGMVILV
                     NYIKSIPKELDEAAALDGCGYFRFVTAVLFPLIRPAVATVAVLHAIGIWNELILPTIY
                     LTNKDYYPITRGMIVFQGVYGSDWPTLAAAVLIMTLPMLLIFLLLQRYIISGLTQGAV
                     KG"
     gene            7716..8435
                     /locus_tag="AC16CB_07645"
     CDS             7716..8435
                     /locus_tag="AC16CB_07645"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HIR3342379.1"
                     /translation="MKSNTWSLVWQDEFDGAEIDKTKWRLDVGYTGESNGELEIYTDR
                     PENVYLEDSCLVIAALEENYRGYRYTSARLTTKDLHSWNYGRVEARIRIPYGQGLWPA
                     FWMLGDNYPTVGWPECGEIDIMENIGKMPDTVRGTLHGPGYCRDDGIWADFTLSTQKF
                     ADDFHLFAVEWEERQIRWYVDNGLYHSLTAEYVPGKWVFDHPFFILLNVAVGGHWPGY
                     PDETTQFPQHMYVDYVRVYSD"
BASE COUNT         2085 a         2112 c         2109 g         2274 t
ORIGIN      
        1 cgccatcatc gacatcccca ccgatccagg ctacgtcacc atcctttaac tcatcgtcac
       61 cttctgggca ttcgctaggc gcgctcaaga atggattgtc gctggaggcg gagtagattt
      121 cttcatcgtc atccgtatcc tcgatcttca ggctcatcga ttcgaagtct tctttgccga
      181 tattgtcgat ctcaaagatg gcgaagggtt ctccgccgca ttcgtggatg ttgttaaagg
      241 aagggtcaaa gcgcacgtcc ggtttcggtg tgaaagtggc tgtgaccggt atgggtgggg
      301 gagcgatcac cggtacagcg gagacttctc cctgtacgct gacaagattt ccaaatatcc
      361 agcagtattg gccgggtctg ccagggattt gcacgtacca ccaggagctg tttgagtttc
      421 gaccatgtat ctcggctttt tgcccttctg ccagcgtggt tatcggcgga tcgtaggcgg
      481 tgctggggcc gttgcggcag ttggagccaa tttggaccgt aatcatagga ccggtcggtt
      541 ccggtctaaa aaaagacgtt ggggtgaact cgggagtcga agtctccgct ggcgtctccg
      601 ttggtggtat gggcgtgttg gttgggaagg ccgttggctg ggctgccgcc gtctcagtga
      661 gtttggcttc caaggtctgt gccgcagctg tgtagacagc ttccacagat acctgtgtgg
      721 atgaacttaa acctgcatca ggatcgaaat tcctcttaag atagagataa cctgctaccg
      781 atagaatgct gcccacgaac agaaacatta gaattagcag taatcgagtt tttttaggtc
      841 tatgccaaga agttcgaata gttggtattt tgtctatttg cttactatca atatacaacg
      901 ggcattgatt atgtgattgg ctttggcaat attgttcctg ataatccaga gttggtgtca
      961 taggtttttg catccgataa cagcggttaa accgtgacgg gaataatata aaggtggtct
     1021 gatcatctat catacccaga aagggacaag tatccattat ttcactcata tttcttgctg
     1081 gtgaaccatt cctatgcctt gtgcaatggc atcgttagga tcgtggatta tagtcttttt
     1141 ccccgaatat agatctcacc ctcgtcggga gaatatagac catacaggat attcatcaaa
     1201 gttgtttttc cagcgccgtt ttctcctaaa agagccagga tttccccttc accgagggtt
     1261 aggtcaatat gatcattggc taaaacgcca ggaaaccttt tggtaatccc ttttaattcc
     1321 aacactggtg caataggatt ctccgtcgca ggcttggggg atttttcccc acaactccct
     1381 gtaattatct tgcaggcggt gccattaatc agattgtttg acaccgcctg cgtttattat
     1441 atcaattttg gcacaggatt accatcagtt ttatgggcgg gggcgtaatt cagttttggg
     1501 ggaaatttaa tacaatcaag ccaaatactc caaggaagcc gagatggaaa agccacgcct
     1561 cgttgagaca ggcagcagtt cattttttgg agaatacctg tatgatcagg tcgtccccca
     1621 aggccacttt ttaacgtcgt ctgaagaaaa tcatcgcatg ggggcggttc acaagcaagc
     1681 taatcagatt gtacaaaaga gacggagtgg ttagggaacc tcagtttgat ccggtattgg
     1741 tttcgcagaa atgtttccaa gttgaagttg aatagcacat tcgtcatcta ttcgacttct
     1801 atccaggttt attatttatc ctttctgtct aattattact tggggaggca tataccgcta
     1861 acagagagct acgagtcgcc tttccttgtt ggtgcaattg tggtgcaatt tgcatagaat
     1921 caatacccat tatagattct atagactttt aaagcgccat atatgggggt gaaaagcggt
     1981 gtatacccat atatatgggg ttacggcctc gctcttaatc aattggttac aggttcgagt
     2041 cctgtcgggg tcacctattt cccccattgg ccctttgtga tctccgctaa tgacctgctt
     2101 tccggcaggt ttttgggtta acacggttcc tgcattttgg cgatatcggg ctaagtagaa
     2161 gtctttcagg gtttagccag aaattcttta acataaggaa ccaccaagtc gctgcgtgga
     2221 ataatattaa agtgattgag ccctggcaga gagaccaagg tgacatttga aagttcttca
     2281 gcacagcgtg caatcagttc gtattggtcg tcttcactac cggcatacaa aagacagggc
     2341 atggtcatac ctggcaggac atccgaaatg tcaggtctgt cttgaataac tgccagcagg
     2401 gcccgggcat cattatttct catccgagcc aggccgtcag tagagtagat gccccaagtt
     2461 tctacaatag ccacccaggt ttcgagccca gtgctaagca gttcccgaag tggttcaaac
     2521 tttattccat agggttgtgc gccaccgatg attaatgcgt tcaatcggtg tctagcgtac
     2581 ttggccatac caaatccaat cgagcccccc atcgagtatc ccaggtaatt tgtcttcttg
     2641 atgtggagat cgtccagcac ggtggtaacg tcggatacaa attgctccaa gtcatattgc
     2701 tcgggttcat aaagtttttc gctctcacca tgcccgcggg catccaccaa aatgaggcgg
     2761 tagtcattac ataactcttc tacataacca aaatcatacc aatccataag gctggcactg
     2821 aatccgtgtt gcaataccag cggcggaccg tccccaatta cttgataatg aatgtgaact
     2881 ccctgattgt ttgcataagg catgataaaa ccctccccga ttgattctca aaatatgcag
     2941 ttgcgaaccc gcttactagg cagttaaggt aaaaatcatg tataggtgta tccgcgccgt
     3001 tggccggttg aaaatcaaag gaactggaga tatgttgatg gcaatgctac gcagggcacc
     3061 ggaaggagat gacggaatct ccgcatcatg gggcggcatt aactaacgcc tgctcaccga
     3121 ttccctgatc accagcgcgg attgaaactt gggtaatgag atggattttc cctggatcag
     3181 atagatcaat gccctaccgg cagcctcgcc gatctccaat ggcgggcgcc ggatggaagt
     3241 caatggcggt accatatagg cagagggagc ttggtcatcg aagccaataa tggacacgtc
     3301 ctcgggcaca cgcaagcccc tgcgatataa agccagccta gctccataag ccaactgatc
     3361 gttggcggca aagatggcgc tgaaaatgcg gccgcgcatg agcaacattt ctacggccat
     3421 cacccccgaa ggctcggtaa aatcaccttc aatgattagc tccggatccg gttgaatccc
     3481 attctgctcg agagccttga tatacccgtc gcgccgttca ttggcatcct gatgatttgg
     3541 cagcccggtg atgtgagcga ttcgacgatg gcccgcatca ataaggtgct gcgtggcttt
     3601 gtaagccgct tccaaatcat caaagggtaa acactgctcg cttagctccg caatttctcg
     3661 ccccacaatg atcatgggaa tccggagagc aatctcgatc aggaattcat cgggtgaatg
     3721 accattcaga acaattaaac cgtccacctg gcgctgcatg aacatataca aagcaatctg
     3781 atctttttca gcatcccaac caccatcggc gaagagaggc gagtaaccgc tgccatcaat
     3841 gcctcgtaag atgccacgca agatgacatc gtagaatggg ctgccgatca gttgggtaag
     3901 cacgccgatg gtcctggatt gcccgccggc caggccttga gcaaagagat ttggtttata
     3961 accaagctcg gtgatgatac tgagcactct ttggcgagtt tcttctgcta caggcgcagt
     4021 attattcaac acacgggaga cagtgctgat tgagacattc gctcgattgg caatatcttg
     4081 aatagtgaaa ttttttcctg attccatcta cacctcaatt gacaattcaa ttatacacag
     4141 aaagcgcttt cagtcaacca ttgcgcaaac gaacttcagc cctatcttta tggcgtaaac
     4201 acaggaaaaa tagattaata cactccatat cctattgact taagataaaa attatgctac
     4261 catcatgaaa gcgctttctg tttgtagaca atatacatct gatttacatg ataacgcttt
     4321 cattctcgga cagtttcacg atgttacaca cggataagca aatcgatata tccaagtgga
     4381 cagttggtct gagttcagga ggtgaaccta aaatacaacg ttttgcacac cccagtaacc
     4441 tttaattccg cgatccagca agatactaaa caataattaa ggagagattt cgagatgaga
     4501 aagaaccttt tagccatcat aattctgcta gtgagcctga gcatggtgct gagcgcctgc
     4561 gcgagccagg ctacaccttc cccagaggca gaagttgaag cgccacctga agaaacaact
     4621 gaggaagtca ctctgagggt cctcgtgcac cagaatcccc ccatggtgga attcatagaa
     4681 gccttcaatg caaagttcat ggaaaaatat cctaacgtaa ctgtagacat gtcggttgtg
     4741 aacgccaatg atttggccac agttacccaa acccgcttaa ccgccaatga cgtagatgtg
     4801 gtcgatatgt ttgcatttac gaatgcggct cagccttata tgaaaaacgt tgagccaccg
     4861 aactggcaaa cattgattga ggctggttac ctgcttgata tctcaggtca gccgtttttg
     4921 gataattact tcccggcttc cattgcagac gcaggcacct acaacggcaa ggtataccag
     4981 atcaatctgg ggactgtgtc ctacagtggc atctattaca acgagtctct ttttgctgaa
     5041 tataatgtag ccgtccccac aacctgggac gagctggttg cagcctgtga aacatttact
     5101 gctgctgaca tcccctgcat gaccgctggc ggtaaagacg gctggcccat cttcgtgggc
     5161 gcatacggcc tgattggctc gatctatccg gaccaggcag ccctggtcga gggtctctgg
     5221 actggctcga tcaagtggaa tgacgccaag tcgatggaaa tgtgggataa gatgaaggtc
     5281 tacgcccaga acatgatgga agccggtgct agcggtattg ccggtgatgc tgctcccgga
     5341 cgttttgcct ccggcgcagt agccatgttc tcaggtggaa cctggtatgc cgccgcaata
     5401 gaagaagcag aaccggattt cgagtggggt tatatcccct tcccgggcag cgacaacccc
     5461 gaagacaaca aatacctgtt tgggaagtac gatcagggct gggtcatcgc agccaattcc
     5521 cccaataccg ataccggcct cctgtacttg gaagaatttt ctgatccagc aaactaccag
     5581 gaatttgtca acgcggttgg tttcttcccc acccagccgg gcgctacatt ggatacccag
     5641 atcggtcaag aagtagcccc ttacctggaa aatttccggg ttgggtatga gcagtattgg
     5701 gtcgcaccaa agggcgctgg ccagtatgcg aacccgtggg cttcctattt ccaaccattt
     5761 ggcgaatttg atgatgcgca ggaattggca gacaaagttc aggcagacct tcaatctggc
     5821 ctggacgcca gccagtaaag aatagcttct tctccagtag gcttctcgtc tgtgatgaga
     5881 agcctacttt tctctaatct atacgaggag gcataccatg atggcttatc acttcggccg
     5941 cggacgggct aggttatctc cttacttatt gctcatcccc ggcatactcc tttaccttct
     6001 tatcgccttg ggtccgtctc tggcaacgag cgtgttttcc ttcacagatg cgaccggtat
     6061 acgcggcgcg cctatccatt ggatcgggct ggaaaattac cgagaattcc ttttactggg
     6121 tcaggcttct cgggataact atgctgccct tggtcggaca ctgattttct gtttctttgt
     6181 gaccactatc cagttcggac tgggcttgct ggtagcgatg ctggtcaacc agcgcttgaa
     6241 aggtacaaac ttttttcgca cgctgtactt catgccagtg atcttgggtg cagtcatcca
     6301 gggcttaatc tggtcgcttt tcctctaccc attaggcggc ccgatggcgc gcatcctgga
     6361 attctttggg acaagttcag agttttttgg aggacaacct aacgaagcct ttgcctatgt
     6421 catcgtcgtc cagatttggg ctaatatggg caccacgatg gtcattttcc tggcgggcct
     6481 gcaaaccatt cctgacgaac tctacgaagc tgcccagatc gatggtgcta gcggctggca
     6541 agtcttttcg aacgtgacct ggcctctgct tacaccgagt gtgaacacca atctgctttt
     6601 aaatatcatt ggttcgctcc aggcgtggca gttattcctg gttttgatcg gttaccggaa
     6661 cgggacccaa gtcttgggtt atttggtata cgcccaaggt tttggtcaaa cctcaggtag
     6721 catggtcaac ccgttccggc aaggatatgc tgcagcagca tccatcgtgc tgtttttcct
     6781 ggtactggtt ctgggaatgg taacccaaac gtttctgcgt cgcagagagg agaggatttt
     6841 gggatgaaca caaccatgaa aacccgccca ccgatcaatt ggggtcgtgt cgcctcctat
     6901 gcagccctag tgctatttgc ggtcatttat gcgggaccct tgctcatgct ggtaaatact
     6961 tccttaaaaa cgctaccagc gttcatgaag gacgccacct ccctggtcac aagcttgcat
     7021 ttcgataact ttatcgaagc ctgggaaaaa gccaacttcc ctcgctatct gaccaactcg
     7081 ctgatctata ccctctcagc cacattaatt tatgtggtta cggcgatatt tgtcgctttt
     7141 ccggttgctc gcggttacgt cagaggtgcc aattttatct taactatgtt cgtgatcgcc
     7201 ttgttcctgc ccccggcatt gattccccag ttccagttga tgttacgtct aggtctatat
     7261 aacaatcccg tgggatacat cttgctgttt accatcaacc ccattggcat ggtgatcttg
     7321 gtgaactata tcaagagtat tcctaaggag ctggacgagg cagctgcctt ggacggttgt
     7381 gggtactttc gctttgttac ggcggttctc tttcccctga ttcggccagc tgtagccacg
     7441 gttgccgtat tgcatgccat tggcatctgg aatgagctga ttctgccaac catctatctg
     7501 accaataagg attattatcc catcacgcgc ggcatgattg tcttccaggg tgtttacggc
     7561 agcgactggc ctaccctggc agctgcggtt ctgattatga cgctaccgat gctgttaatt
     7621 ttcctgctat tacagcgcta catcatctct ggtcttaccc agggagccgt caaaggataa
     7681 aagagcacaa aagcttccag cggacaaacc tctttatgaa atccaatacc tggtcacttg
     7741 tctggcagga tgaattcgat ggtgctgaaa tcgacaaaac caagtggcgt ctcgatgttg
     7801 gatacacggg cgagagcaac ggtgaattgg agatttacac cgatcgacct gaaaacgtct
     7861 atcttgaaga tagctgcctg gtcattgcag ccctagaaga gaactaccga ggatatcgat
     7921 acacttctgc ccgcctgacg acgaaagacc tgcacagctg gaattacggc agggtggagg
     7981 cgcggatcag aattccttat gggcaaggtc tctggccggc tttctggatg ctgggagata
     8041 actacccgac cgtcggctgg ccggagtgtg gggaaattga catcatggag aatattggaa
     8101 aaatgccaga caccgtgcgc ggcaccctac acggtccggg ctattgccgt gatgacggta
     8161 tctgggcgga tttcaccctc agcactcaga aattcgcgga tgacttccac ttgtttgccg
     8221 tggaatggga agaaagacag atccgctggt atgtcgataa tgggctttat cattccctca
     8281 ccgccgagta tgtccccggg aaatgggtgt ttgaccaccc ctttttcatc ctcctaaacg
     8341 tagccgtggg cggccactgg cctggttatc ccgatgaaac cacacagttc ccacagcaca
     8401 tgtatgtgga ttatgtacgg gtttattccg attgaaggcc tatctcctct gaaagcgggt
     8461 ttggtaaatt tcatgttgat gttgaggaga aactctatgc cctctgggtt gctacaccgc
     8521 gggtttatca tatttatcag cgcgatgatg ctgcttaccg caagcatatc ttgcggaccg
//
LOCUS       DBNGSX010000251         2811 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_712295, whole genome shotgun sequence.
ACCESSION   DBNGSX010000251 DBNGSX010000000
VERSION     DBNGSX010000251.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_712295"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="AC16CB_07650"
     CDS             complement(<1..554)
                     /locus_tag="AC16CB_07650"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3342380.1"
                     /translation="MLHNFGVLWDMDGVLVDTAEFHFRSWEQTLREMDIPFERRKFQA
                     VFGRNNYDTLTSLLHRPPEAELLQAVSEQKESLFRKLIHGKAIPLPGVRTWLEHLRHL
                     GALQAVASSAPQENIDLLVDELGLHSYFATLCAGHMLPSKPDPALFLLAASILGLSPR
                     VCIVIEDSLAGVEAAHRAGMKCLAV"
     gene            732..2570
                     /locus_tag="AC16CB_07655"
     CDS             732..2570
                     /locus_tag="AC16CB_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR3342381.1"
                     /translation="MFTRLWQLLVGSPLPTQELAHKRLNKIRALAAFSPDALSSIAYA
                     NQEIFLGLVVAGSAGLIFSFPISATIALLLCIVAASYYQTIHGYPSGGGSYIVARHNL
                     GTLPGLVAGAALMIDYVLTAAVSLTAGVEAMASAIPELWPYRMQAALFILAVITLINL
                     RGTQESGTAMALPVYGFLIIYIIMILYGIYQAIIQGPGTLVSTAPPASQPLTIFLVLH
                     AFASGSTALTGIEAISNGVPAFKPPEARNAGRTLIAMALLMGFLFVGSMGLTQYFAVV
                     AGPQETILSALARRLLGNGILYYLVQASTLLILAVAANTSFTGFPRLASIMARDGFLP
                     RPLYNLGERLVFSNGMLVLAAVAGVLIIAFGGDSHSLVPLFAVGAFLAFTMSQAGMVV
                     HWLRERGAGWWLKSAINGTGALVTATTLGIVAISKFVHGAWFTLVLIPSLVYIFMKIK
                     GHYTEVARELTLRGLPPSLRPAPKMRLVVPISGVHRGVINAVNYARSISENITAVYIE
                     IDPGSGEKIQERWEEWFPDLSLVIRPSPYRSTIRSLLDFLDSYDIESNDGQLAAVVLP
                     EIVPAKNWQTLLHNQTAWLIKAALLYRRRHLGYQRVIIDVPFHLKK"
     gene            2585..>2811
                     /locus_tag="AC16CB_07660"
     CDS             2585..>2811
                     /locus_tag="AC16CB_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HIR3342382.1"
                     /translation="MKILALEQDIPGVTSEQFQPYLKAEAAKVWELNQAGVIRETYFR
                     QDRASAILVLECADEENAKRILGTFPLVRAG"
BASE COUNT          567 a          859 c          779 g          606 t
ORIGIN      
        1 accgcaagac atttcatgcc cgcccgatgg gcagcctcta cccccgccag cgagtcctcg
       61 atgacaatac acacccgcgg cgaaagtccc aatatgctcg cagcgagcaa aaacaaggct
      121 gggtccggct tgctcggcag catgtggcca gcgcacaagg tcgcgaaata ggagtgtaac
      181 cccagctcat ccaccagcag atcgatgttc tcttggggag ccgaggaggc caccgcctgt
      241 aaagctccca ggtggcgcag gtgctccagc catgtccgca cgcctggcag gggtatagct
      301 ttcccatgga tcagctttcg gaacagggac tccttttgct cgctgaccgc ttgtaaaagc
      361 tctgcttcag gagggcgatg gagaaggctg gtcagcgtat catagttgtt gcgcccaaat
      421 actgcctgga actttctccg ctcaaagggg atatccatct ctctcaaggt ctgttcccac
      481 gagcggaagt gaaactcggc ggtatccacc agcacgccat ccatgtccca gagcacgcca
      541 aaattgtgta acatcttttc aggcccttag cgtaataaag acttggcagg gattggttgg
      601 gtttttcatc ccagctacaa tcaattatat cgatcaacgg tggcataggc attccacagg
      661 atgacacccc cgcagaaaca caaaagcgga gtacacttaa gcatcaaatc ctatctggca
      721 caggagcgaa attgtttaca cgcctttggc aacttctggt gggctcgccg ctgccaaccc
      781 aggagctggc tcataaacgg ctgaacaaga ttcgggcgct agctgctttc tcgccggatg
      841 ccctgtcgtc catcgcctac gccaatcagg agatctttct ggggttggtg gtggcaggca
      901 gcgcagggct gatcttctct ttccccatca gtgccaccat cgccctgctg ctgtgtattg
      961 tggcggcctc ctattaccag actatccacg gctacccgtc cggcgggggt tcctatatcg
     1021 tcgcccggca taatctggga acacttcccg ggctggtggc gggggcggcc ctgatgatcg
     1081 attatgtgct cacggcggcg gtcagcctga cggcgggcgt ggaggccatg gcttccgcca
     1141 tacctgagtt gtggccctac cggatgcagg cggctctgtt tatactagcc gtgatcaccc
     1201 tgatcaacct gcgcggcacc caggaatctg gtacagccat ggcgctgccg gtttacgggt
     1261 tcttgatcat atacatcatc atgatcctgt acggcatcta ccaggccatc atccagggac
     1321 cgggcacgct ggtaagtact gcgccgcccg cctcccaacc gctgacgatc ttcctggtgc
     1381 tgcacgcctt cgcctccggc agcaccgctc tgaccggcat cgaggccatc tccaacggcg
     1441 taccagcctt caaaccgcca gaggcgcgca acgccgggcg cacactgatc gccatggcct
     1501 tgctgatggg ctttctcttc gtgggaagca tggggctcac acaatacttc gccgtggtag
     1561 ccgggccaca ggagaccatc ctctccgccc tggcgcgtcg cctcttaggg aacggcattc
     1621 tatattacct ggtgcaagcc agcaccctgc tcatcctggc tgtggctgcc aataccagct
     1681 ttaccggctt cccgcgcctg gcttccatca tggcgcggga tggctttctg cctcgcccgc
     1741 tgtacaacct gggcgagcgg ctggtattct cgaatggcat gttggtgctg gctgctgtcg
     1801 ccggtgtgct gattatagcc ttcggagggg attcgcactc cctggtgcca ctgttcgctg
     1861 tgggggcatt tctggctttt accatgtccc aggctggcat ggtggtgcac tggctgcgcg
     1921 agcgtggcgc aggctggtgg ctaaagagcg caatcaacgg gaccggcgcg ctggtcacag
     1981 cgacgacgtt aggcattgtt gctatcagta agttcgtaca cggtgcctgg ttcaccctgg
     2041 tcttaatccc ctcgttggtc tacattttta tgaaaatcaa aggccactat actgaagtag
     2101 cccgcgagct gaccctgcgc ggccttccgc cctccttgcg cccggcgccc aagatgcgcc
     2161 tggtcgtccc catctcgggg gtgcaccggg gagtcatcaa tgcagtcaac tacgcccgct
     2221 cgatttccga aaacattacc gccgtctata tcgaaatcga tcccggaagc ggagagaaga
     2281 tccaggagcg atgggaagaa tggttccccg acctaagctt ggtcatacgg ccctcaccat
     2341 atcgctcgac catcagatca ttgctggatt ttctcgacag ttacgacatt gagagcaatg
     2401 acgggcaact ggctgccgtg gtgctgccag agattgtccc tgccaaaaac tggcagactc
     2461 ttttacacaa ccagaccgcc tggctgatca aagccgccct gctctaccgc cggcggcacc
     2521 tggggtacca acgtgtgatc atcgacgtgc cgtttcacct gaaaaaatag attaggagcc
     2581 tagtatgaaa attctcgccc tcgagcagga tatacctggt gtgaccagcg agcaattcca
     2641 accctacctt aaagctgagg ctgccaaggt ctgggagctc aaccaggcag gcgtgatccg
     2701 cgaaacttac tttcgccagg accgggcaag cgctatcctg gtgctcgaat gcgccgatga
     2761 ggaaaacgcc aagcgcatcc tgggcacctt cccgctggtg cgggcagggt t
//
LOCUS       DBNGSX010000252         9784 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1267085, whole genome shotgun sequence.
ACCESSION   DBNGSX010000252 DBNGSX010000000
VERSION     DBNGSX010000252.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9784)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9784)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9784
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1267085"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            430..645
                     /locus_tag="AC16CB_07665"
     CDS             430..645
                     /locus_tag="AC16CB_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342383.1"
                     /translation="MPPASCSHLHGIVSWRVSHAQQVEQALYPMLSLLGYSQYFAFLP
                     TILDAERFNMDRCHTPHALDPFEDEAG"
     gene            complement(758..1717)
                     /locus_tag="AC16CB_07670"
     CDS             complement(758..1717)
                     /locus_tag="AC16CB_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR3342384.1"
                     /translation="MKKFLGCDLGGTNLRAAIVDVETGEVLHQMSIPTLAREGHDAVM
                     KRMADLLLQVIQSAGMQKDDIGGIGIGVPGVLDLEKGETLFLPNLPGTWPHVPLRDTI
                     TQLTGLPTALLNDVRSITNSEWRFGAGRGVDTIAVFAIGTGIGGGLVVNGQLHLGIGG
                     TGGELGHTIIDFNGPRCGCGNNGCLEAYASGPAIAAMGMKAVAQGLTTNIAELCEFDL
                     NCITPELIAKAAQAGDEIAKEIYEKAGFYIGIAASNVCAAVGPRRIVIGGGVAKAGEL
                     LLDPIRHTIKKRVYVMPVEEVEVVPSQLGDNAGVIGVACWAAL"
     gene            complement(1827..2741)
                     /locus_tag="AC16CB_07675"
     CDS             complement(1827..2741)
                     /locus_tag="AC16CB_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR3342385.1"
                     /translation="MKLGFFTAALPGNTLVQAAQWGTQSGFQAIEIACWPLEKATRRY
                     AGVTHINVAELDNVQAKEIRKMLDGYGLTISSLGYYPNPLHPDPEHRETVTAHLKKVI
                     EAAEMLEVPIVGTFIGKDKDKTVPQNLEDYAKIWPPIVKFAKEHGIKIAIENCPMIFS
                     YDEWPGGNNLASTPAIWRKMWEIIPEDNFGLNLDPSHLILQMIDYERVIHDFADKIFH
                     VHAKDLHIDREGLYHNGVLSQGMGWQIPRLPGLGDMDWGKFFAALTAVGYDYVVSIEH
                     EDRAFEGDEELIKRGFYLSRDVLKPYIH"
     gene            complement(2845..4008)
                     /locus_tag="AC16CB_07680"
     CDS             complement(2845..4008)
                     /locus_tag="AC16CB_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3342386.1"
                     /translation="MTKQLTKQTIGAGVAGTGFIGPAHIEGLRRNGIQVLGLAEITKA
                     KAEQKATELGIPRLYGSLDDMLADPDIDVVHLATPNYLHHSHAKAALLAGKHVVCEKP
                     LAMNATESAELVELAAERRLVNSVNFNIRMYPLVQQARSIVQNGELGDLFILQGSYLQ
                     DWLLFPTDWNWRLEPSLGGTLRAVGDIGSHWFDLLTFITGLRVEEVYADFKTFHPIRK
                     KPAKPLETFTGKILTPEDYIDQPIHTEDYATILLHYEKGVRGVLTVSQVSSGRKNRLF
                     YEINGSKSSLGWNSERPNELWIGHRTEPNQTLMKDPSLLSLEARETASYPGGHNEGFP
                     DTFKQLYTKIYNYILAGNYTKTPDFPTFADGHYEMLLCEAIERSAKEGKWVKV"
     gene            complement(4021..4761)
                     /gene="deoC"
                     /locus_tag="AC16CB_07685"
     CDS             complement(4021..4761)
                     /gene="deoC"
                     /locus_tag="AC16CB_07685"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIR3342387.1"
                     /translation="MTLNSKTVTYEQLAKVIDHSLLRPELTEADIVAGCELAARYHTA
                     TVCVKPCHVKLAKEILKDSDVLVSTVVGFPHGSNLTAIKVAEAQQAMDDGAVELDMVL
                     NIGQLRSGKTDYVRDDIKEVCDAAHRRSVKVKVIFENAYLTDEEKITACKLCEEAGAD
                     WVKTSTGFAPSGATLDDLRLMRANVSQKVQVKAAGGVRTLPDLLEVIDVGVTRCGATA
                     TATILDNFKALQEGKKVEIKPNATPGAY"
     gene            complement(4786..5865)
                     /locus_tag="AC16CB_07690"
     CDS             complement(4786..5865)
                     /locus_tag="AC16CB_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3342388.1"
                     /translation="MSKTVKIGMIGTGFVGDIHHAAFKGWVHNADLVAVASPNNAANF
                     AKERGIPDAYSDYREMLKDKEIDVVDIGIPNDLHCEAVVAAAQAGKHVIVEKPLCVTL
                     EEADEMIEACKKAGVLLMYAEELLFAPKYVRVKMLIDEGAIGEPFLAKQSEEHPGPHM
                     PWFWDVNRSGGGVLLDMGCHSIEYTRWVLGKPKVKSVTAFMGTYVHQGRTQGEDHSYC
                     IIEYEGNKVAMIENSWAKGGGVDDRCEIYGKKGHTRADLLRGSSLLTYSEEGYGYAVE
                     KAGQTKGYTFTMFEEIWNYGFPQEMQHFVDSVLGKVTCMETGEDGREVLKIMYAAYQS
                     AGEGRKIGFPYDPPKVKKPIDLWKR"
     gene            complement(6036..7004)
                     /locus_tag="AC16CB_07695"
     CDS             complement(6036..7004)
                     /locus_tag="AC16CB_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3342389.1"
                     /translation="MKRFYISPPVAALLLAVVLFVLSGLLPNSYGSDLNVARSQALNI
                     LRLSVFLGVIAAGQTLVIISGFEGIDLSAGSIVTLTAIFTYVIVKGEDNKVLLALLVS
                     LAVGAFIGFLNGAGITFLKISPFVMTLGMSGVVTGAIILANHGNVSGKVAPIMTRVIA
                     RPLSPSFPIPNALIIWLIFGVLMWLLLERTAFGKNLFAIGVNRVTAKLSGVNVIAMNL
                     ATYTLAGVLAGFGGFLVVGNTGVVLIQLGQPFLFPSIAAVAVGGTLLSGGKGSYWGTM
                     ADAIVLTLITSLLTTMQMPDSVRRMVLGTTLLVLISIYGRQQALRQ"
     gene            complement(7001..7969)
                     /locus_tag="AC16CB_07700"
     CDS             complement(7001..7969)
                     /locus_tag="AC16CB_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3342390.1"
                     /translation="MKKRITWDFLITRYPAFFPLILLMLSIAINLFLQPNMFDLETLN
                     SNMRVFLPLILMAVGQAIVLLGGGIDISVGGIVSIVNTILATRVGLDGSPEKMWQFVL
                     ISLLAGVLAGAINGFFIAFLRLQPIITTYATSFLYAGFALFLLPNPGGGIPADIANLY
                     RTSTPLGLPLAFYVIAILLLIWIYVSSTRYGKYLFAVGGKADAAYETAVPVTLMQFST
                     YVISGFMAALAGIAITMLSGSGNADIGNPMTLNSITAVVIGGNAFSGGIGGVAGPVMG
                     AVTLSLIQNIISFAHIDTWWETLVKATIIVVALATPGIINLFRRRE"
     gene            complement(7966..9474)
                     /locus_tag="AC16CB_07705"
     CDS             complement(7966..9474)
                     /locus_tag="AC16CB_07705"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR3342391.1"
                     /translation="MAKSLSARNIVKRYGGVVALSDGNLDVQTGEVVALIGANGSGKS
                     TMSKVINGVVVLDGGQLLLDDKTVHFSSPQAAKNMGIATVFQELSLIPHMTVAENIWL
                     TREPMTPLGTVNRKAIIEQTEQLLSLFRGTYKTDLRPDDPVASLPPDEKQIIEILKAI
                     SFNPWMVILDEATASLDSRQVQRLFELVRNWKEVGKAIVFVSHRMEEIFRIADKYSVL
                     RNGKTVGAGNMEDINEKELVKLMIEKESVFTFTRGETKVNEKPVCLEVKDLRTDSLRG
                     VSFSVREGELVGIGGLRGQGQRDLLLSLFGDVPYSGQVCLFGNECRLRHPREAMKLGL
                     ALVPGDRSREGLLYIRSILENLQLPSWAQYGFPLRIGKAKKDAEQAGANLNLIMAGLG
                     EPVSSLSGGNAQKVVLGKWLMRKPKLLLLDDPTKGVDVGTKAEFYALLTQLCSEGKTI
                     LFYSSDDEELVGLCDRVLVIHDGIIRSELSGATLTKENLIAASLGGSNGGAL"
     gene            complement(9481..>9784)
                     /locus_tag="AC16CB_07710"
     CDS             complement(9481..>9784)
                     /locus_tag="AC16CB_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR3342392.1"
                     /translation="QYLKLWQEALTMNPDFDSIAVANHPGVSPTGLRIAVNMLQGKKV
                     DESKLGGVNGLSFVIPLAAVITSENLDEGLAMCEGQPDAYLLDDIMTDEEVQQYFK"
BASE COUNT         2296 a         2678 c         2468 g         2342 t
ORIGIN      
        1 atctcccaaa taaccagccc actgtaagtc tctcttccgg aagatatctt tacaaaacca
       61 tggactaggc tctgcttcag gggcgattat atccaaatcg ccaactatta gagcagctat
      121 agtagcaaag tcttgaataa tggtctgatt tttgtcacta tatacatcca tttttccctc
      181 cggcccatac atcagaaacg gtcatgctaa caacattatt gcactcgctc atacttgagt
      241 caagtaggtc tgcttagagt ttccttccac atataaaaat cacccccgtc gggtagtcac
      301 gtgattatac aaggggctag cgcgacccta aagtgaacat ggggggtgca caactgaata
      361 tctatcccct tggtgttggt aatcccgtag gataccgcat gggctccagg gggtggggtg
      421 taggccaccg tgccacctgc atcctgcagt catctgcacg gcatcgtatc ctggcgcgtc
      481 tcgcacgctc agcaagttga gcaagcactt tatcccatgc tttcgctcct tggatactct
      541 caatactttg cctttctgcc aacaatcctg gatgccgaac gattcaatat ggaccgctgt
      601 cacacaccac atgccctcga tccgtttgaa gacgaagcgg gataactacg tttcgattga
      661 aagcataatg ataacgactt aggtatttag gcagtggtta aagccaaggc cagatgccaa
      721 ttaataatcc gtttgcaggt ttcattgcat taaacagtta gagagctgcc cagcaagcca
      781 caccgataac gccagcatta tctcccaatt gtgaggggac aacctccacc tcctctactg
      841 gcatgacata cacgcgcttt ttgatagtgt gcctgatggg atcgagcaaa agttcgcctg
      901 cctttgccac accaccacca atgacgatac ggcgtggtcc aacagctgcg catacgttag
      961 aagctgcaat accgatataa aaacccgcct tttcgtatat ttctttggca atctcatcac
     1021 ctgcctgagc tgctttggca atcaattccg gggtgatgca gttcaggtcg aactcacaca
     1081 gttcagcgat atttgtagtt aagccctggg caacggcttt catgcccatc gcagcaattg
     1141 cggggccgga ggcatatgcc tcgaggcaac cattgttgcc acagccacat cgtggaccgt
     1201 tgaagtcaat tatggtgtga cccagttcgc cccctgtgcc accgatgccg aggtgcaatt
     1261 ggccgttgac caccaatccg ccgccgatcc cagtgccgat cgcgaagaca gcgatagtgt
     1321 ctacaccgcg tccggcccca aagcgccact cgctgttcgt gatggaacgg acatcgttta
     1381 gcagagcagt tggaagtcca gtaagctgcg tgattgtatc ccgcagaggc acgtgtggcc
     1441 atgttccagg caggttgggt agaaaaagcg tttcgccctt ctctagatcc agcacaccgg
     1501 gtacaccaat accgatacca cctatgtcat ctttctgcat gccagccgat tggattacct
     1561 gcagcaacaa gtcagccatg cgtttcatga cagcgtcgtg tccctcacgt gccagcgtgg
     1621 ggatactcat ttggtgaagg acttccccag tttcgacatc tacaatggca gcacgcaggt
     1681 tggtaccacc tagatcgcag ccgagaaatt ttttcatcag acatccttgc tggaatcgct
     1741 caccctgccc atctccttgc atataatccc gtaagggatg agggagaaag ggaaatagtg
     1801 agggcaaagt gcaaacttac aaacgattag tgaatgtatg gtttcagcac atcgcgcgac
     1861 aggtagaaac cgcgcttgat cagctcctca tccccttcaa aggcgcggtc ttcatgctca
     1921 atactgacca cgtaatcata gccaacggca gtcagggcgg cgaagaattt gccccagtcc
     1981 atgtcaccca aaccgggcag gcgcggaatt tgccagccca tgccttgtga gagcacaccg
     2041 ttgtgataca gaccctccct gtcaatgtgc aggtctttcg catggacgtg gaatattttg
     2101 tccgcaaaat cgtgaataac acgctcgtag tcaatcattt gcaagatcag gtgcgacgga
     2161 tcaaggttca gcccgaaatt gtcttccggg atgatttccc acatcttgcg ccagatggca
     2221 ggcgtgctgg ccaggttgtt gccgccaggc cattcatcgt aggagaagat catcgggcaa
     2281 ttctcgatgg caatcttgat gccatgctct ttggcgaact tgacaattgg cggccagatt
     2341 ttggcgtaat cttccagatt ctgtggcacg gttttgtctt tgtccttgcc gatgaacgta
     2401 ccgacaatag gcacttccag catctcggcg gcttcgataa ccttcttcag gtgagcggtc
     2461 accgtttcac ggtgctcagg atcggggtga agtggattcg ggtaatagcc aagcgaggaa
     2521 atcgttagac cgtagccatc cagcattttg cggatttcct tggcctggac attgtccaat
     2581 tcggcgacgt taatatgcgt aacacccgcg tagcggcggg tggctttttc gagtggccag
     2641 cacgcaatct cgatggcctg gaatccgctt tgggtacccc attgggcggc ctgtacaagc
     2701 gtattgccgg gtagagcggc ggtgaagaaa cctaatttca ttgatacctc cttgtgtttg
     2761 gagtccaacg attccattcg ttggcgcttg tccaaagtca agcccttaca ggatgcttcg
     2821 cgaagacttt ggactttgga ttgtttagac tttaacccat ttcccttcct tggcgctacg
     2881 ttcgatggct tcgcagagta gcatttcgta atgaccatcg gcgaaggttg gaaagtcggg
     2941 cgtttttgtg taattgccgg ccagaatgta attatatatt tttgtataaa gttgtttgaa
     3001 tgtatcggga aagccctcgt tgtgacctcc gggataagaa gcggtctctc gtgcttcaag
     3061 agagagcagc gaagggtctt tcatcagtgt ctgattgggt tctgtccgat gaccgatcca
     3121 taactcgttg ggcctttccg agttccaacc caacgaagac ttggaaccgt tgatctcata
     3181 gaacaggcgg ttcttgcgcc ctgaagagac ttgcgagacc gtcagaacac cgcgcacacc
     3241 tttctcgtaa tgcagcagga tagtggcgta atcttcggtg tgaatgggct ggtcgatgta
     3301 atcttcaggc gtgaggattt ttccagtgaa ggtttccagc ggtttggcgg gtttcttgcg
     3361 aatggggtgg aaggttttga agtcagcata gacttcttca acgcgcaggc cagtgataaa
     3421 ggtcagcagg tcgaaccaat gggagccgat gtcgcccaca gcgcgcagag tcccacccag
     3481 tgacggttca aggcgccagt tccagtcagt gggaaagagc agccaatctt gcaggtacga
     3541 tccctgcagg atgaacaggt cacccaattc accattttgc actatactcc gcgcttgttg
     3601 aacaagggga tacatgcgga tattgaagtt gaccgagttg acgagtctcc tctctgccgc
     3661 cagttcgacc agttcagccg attcggtggc attcatcgcc aatggcttct cgcagacaac
     3721 gtgcttgcca gccagcaaag cggctttggc atgcgagtgg tgcaggtaat ttggcgtggc
     3781 aagatgcacg acatctatat caggatcagc caacatgtca tccagcgaac cataaaggcg
     3841 cggaatacca agttcagtgg ccttttgttc ggcttttgct ttcgtgattt cagccaatcc
     3901 caacacctgg atgccattgc gacgcaagcc ttcaatgtga gccgggccaa taaagccggt
     3961 tcctgctacg ccggcgccga tggtttgctt ggtgagctgc tttgtcattg tggaatctcc
     4021 ttagtatgct ccgggtgtcg cattcggttt gatttctact ttcttgcctt cctgtagcgc
     4081 tttaaagttg tccaggatgg tagccgttgc agtggcacca cagcgggtca cgccaacatc
     4141 gatcacttcc aggaggtcgg gcaatgtgcg gacgccgccc gccgctttga cctgtacctt
     4201 ctggctgacg ttagcgcgca tcagacgcaa gtcatccaga gtcgcgccgc tgggagcaaa
     4261 accggtggag gtcttgaccc agtctgcgcc tgcctcttca caaagtttgc aggctgtaat
     4321 tttttcttca tcggttagat acgcgttctc gaagatgacc ttgaccttca cgctacgcct
     4381 atgggcagca tcacagacct ctttgatgtc atcacgtacg taatcagttt ttcctgagcg
     4441 taactgaccg atgttcaata ccatatctag ctcgaccgcg ccatcgtcca tggcttgctg
     4501 ggcttcggcg accttgatgg cggtcaggtt gcttccatgt gggaatccca ccacggtgct
     4561 gaccaacaca tccgagtcct tgagtatttc tttggcaagt ttaacgtgac aaggttttac
     4621 gcagacggtg gcggtatggt agcgggcagc cagttcgcaa ccagcgacga tgtccgcttc
     4681 ggtcaattcc ggacgcaaaa gagaatggtc aatgaccttg gcaagctgtt cataagtgac
     4741 ggttttgcta tttaaagtca taacatggac tccaaaatct ccagattacc ttttccacaa
     4801 atcaatcggc ttcttgactt tcggcgggtc gtaggggaac ccgatcttac gcccctcgcc
     4861 agccgattgg taggcggcat acatgatctt caatacttca cgaccatcct cgccagtctc
     4921 catgcaggtg actttgccga gcacactatc aacaaaatgc tgcatctctt gcgggaagcc
     4981 atagttccag atctcttcga acatggtgaa ggtgtagcct ttggtctgtc cggctttttc
     5041 cacagcgtaa ccataccctt cttctgagta ggtcagcaac gaactgccgc gcagcaagtc
     5101 ggcgcgcgtg tgaccttttt tcccgtaaat ctcacaacgg tcgtccacgc cgccaccttt
     5161 agcccaactg ttctcgatca tggcgacttt gttaccctca tattcgatga tgcaatagga
     5221 atggtcttcg ccctgcgtgc gtccttggtg gacgtaagtg cccataaaag cggtcacgct
     5281 cttgactttg ggtttgccca gcacccagcg ggtgtattca atcgagtggc aacccatatc
     5341 gagcagcacg ccgccgccgg aacgattcac gtcccagaac cagggcatgt gcggaccggg
     5401 atgctcctca gattgtttcg caaggaaagg ctcaccaatc gcgccttcat cgatcagcat
     5461 tttgacacgg acgtatttcg gggcaaacag cagctcttcc gcatacatca gcaaaacgcc
     5521 cgcttttttg caggcttcaa tcatctcgtc cgcttcttcg agcgtgacac acagcggttt
     5581 ctcgacgatg acgtgtttgc cagcttgagc ggccgccact acagcttcgc aatgcaggtc
     5641 gttcggaatg ccgatgtcta ccacatcaat ttctttatct tttaacatct cgcggtaatc
     5701 gctatacgcg tctgggattc cgcgctcttt ggcaaaatta gcagcgttat tgggactggc
     5761 aactgcaacc aaatccgcat tatgcaccca gcccttaaaa gcagcatgat gaatatcacc
     5821 gacgaaaccg gttccgatca taccaatctt taccgttttg ctcatggcat tctccttcct
     5881 tcaatgaatg ttcacaggcc tttgcctgaa agagttattt gagaataagc gagcgcgtca
     5941 atctgcaatg gaaactgcgg tgggagctgc aatgaagtat ccgatgtaag ctctcaaggg
     6001 gttgtgatag gatacgagcc ctgcctattt ccgaattatt gccgcaaggc ttgctggcgg
     6061 ccgtagatag agatcaatac tagtagtgtt gtgcccaaca ccatacggcg gacagaatcg
     6121 ggcatctgca tagtggtcag caagctggtg ataagcgtca acacgattgc gtcggccatc
     6181 gtgccccagt agctgccctt cccgcccgag agcagtgtcc cgccgaccgc caccgccgct
     6241 atggatggga acaggaacgg ctgaccaagt tgaatcaaca ccacgcctgt gttcccaacc
     6301 accaggaacc caccaaaccc agccagtaca cctgccaggg tataggtcgc caggttcatc
     6361 gcaatcacat tgacgccgga gagtttggcc gtcactcggt taacaccgat ggcaaacagg
     6421 ttcttgccaa aggccgtgcg ttccagcagc agccacatca ggacgccaaa aatcagccag
     6481 atgatgagcg cattcgggat gggaaaactc ggcgaaaggg gtcgcgcaat cacccgtgtc
     6541 atgatggggg ccaccttccc gctcacgttg ccgtgattgg cgaggataat cgccccagtg
     6601 acaacgcccg acatgccgag cgtcatcaca aacggggata ttttcaaaaa ggtgatacca
     6661 gcaccgttca aaaagccaat gaaagcgccc acagcgaggg aaacgagcaa ggccagcagc
     6721 accttattat cctcgccttt gacgatgacg taggtaaaga tggccgtcag cgtgacaatg
     6781 gaacccgccg acaagtcaat cccttcgaag ccactgatga tgaccagggt ttgccctgcc
     6841 gcaatcacac cgagaaagac cgacaggcgc aaaatattca aggcttgaga gcgcgcaaca
     6901 tttaaatcgc taccgtagct attaggcagc aggcctgaga gcacgaatag cactactgcc
     6961 agcagcaatg cggcgaccgg gggtgagata taaaaacgct tcattctcgc ctcctgaaca
     7021 ggttgatgat gcccggtgtc gccagcgcga ccacgatgat cgtcgcttta accagcgttt
     7081 cccaccatgt gtcaatatgc gcaaacgaaa ttatgttctg gatcaggctg agtgtgaccg
     7141 cgcccataac cggcccagcc acgccgccga tgccaccgct gaaagcgttc cctccaatga
     7201 ccactgcagt gatagagttc agagtcatcg gattaccaat atccgcattg ccagacccac
     7261 ttaacatggt gatcgcaatt ccggccagcg ccgccatgaa gccagagata acataggtgc
     7321 tgaattgcat cagcgtcacc ggcacggcag tttcgtaggc tgcatccgct ttgccgccta
     7381 cagcgaatag atattttcca tagcgggtgg aactgacgta gatccagatc agcaataaga
     7441 tggcgatcac atagaaagct agaggtaggc ccaggggcgt cgaggtgcga tataagtttg
     7501 caatgtccgc aggaatgccg cctcctggat ttggcaggag gaagagtgca aacccggcgt
     7561 acaagaagct agttgcgtac gtagtgataa tgggttgcaa acgcaaaaag gcgatgaaaa
     7621 aaccgttgat tgcgcctgcc aatacgcctg ccagcaggga aatcagcacg aactgccaca
     7681 tcttctccgg cgagccatcc aggcctacac gagtcgccag gatggtgttt actattgaaa
     7741 caattccacc gaccgaaatg tcgatcccgc cacctagcag cacaatcgcc tgccccaccg
     7801 ccatcaggat tagcggcaag aagacgcgca tgttactgtt caacgtttcg aggtcgaaca
     7861 tattgggctg caggaacaaa ttgatggcaa tggacagcat caacaaaatg agcgggaaga
     7921 acgccgggta gcgagtgatc aggaaatccc aggtgatgcg ttttttcata gcgcacctcc
     7981 gttcgaacca cccaggctgg cggcgatcag gttttctttg gtcaaagtcg cacccgacaa
     8041 ctcggagcgg atgatgccgt cgtggatcac cagcacccgg tcgcacaggc ccaccagctc
     8101 ttcatcatcg ctgctataaa acaaaattgt tttcccttcg ctgcataact gtgtaagtag
     8161 cgcgtaaaac tcagctttcg tgcccacatc cacgcctttg gtcgggtcgt ccagcaaaag
     8221 aagcttcggc ttgcgcatca gccacttacc aagtacaact ttctgtgcat tcccacctga
     8281 aaggctactg accggttccc ccagcccggc cattatcaag ttcaggttag cgcctgcctg
     8341 ttctgcatct tttttcgcct ttccaatgcg cagtgggaaa ccatactgcg cccaggacgg
     8401 caattgcaga ttttctagaa tggaacggat gtagagcaga ccttcccggg agcgatcacc
     8461 ggggacaagt gccagcccaa gcttcatcgc ctcgcgcggg tgccggagcc tgcactcatt
     8521 accgaagaga cacacttgcc cagagtaggg cacatcgccg aacagcgaga gcagcaggtc
     8581 acgctgacct tgcccgcgca acccgccgat gcccaccagt tcgccttccc gcacgctaaa
     8641 actgacaccg cgcagcgagt ccgtccgcag gtcttttact tccaggcaaa ccggtttttc
     8701 attgaccttt gtttcgccac gcgtaaaggt gaataccgac tctttctcaa tcatcaactt
     8761 gactagttct ttttcgttga tgtcttccat atttccagcg ccgacagttt taccgttgcg
     8821 caagacactg tatttgtctg caatgcggaa gatttcctcc atacggtgcg agacaaacac
     8881 aattgcctta cctacctctt tccaattcct caccagctcg aagagacgct gcacttggcg
     8941 gctgtccaag ctggcggttg cctcgtcgag gatgaccatc cacgggttga agctgattgc
     9001 tttgagaatt tcgatgattt gtttttcgtc cgggggtagg gaagcaactg ggtcgtctgg
     9061 ccgtagatcc gttttatatg ttccccggaa aagggataat aattgctctg tctgctctat
     9121 gattgctttg cggttaacgg ttcctagagg cgtcatcggc tcgcgcgtca accagatatt
     9181 ttcggcgacg gtcatgtgtg ggatcaggct cagttcctgg aacacggtgg caatccccat
     9241 gttcttggct gcctgcggag aagagaaatg cacggttttg tcatcgagga gtagttgccc
     9301 tccatcgagg acaactactc cattgatcac cttactcatt gtgcttttgc cacttccgtt
     9361 tgcacctatc aacgctacga cttccccggt ttgaacatcc aggttgccat ctgagagtgc
     9421 tacgacaccg ccgtagcgtt tgacaatatt acgggcagaa agcgacttag ccatgaagat
     9481 ttatttgaaa tactgctgga cttcttcgtc ggtcatgatg tcgtcgagca ggtaagcatc
     9541 tggctggcct tcgcacattg ccagaccttc atccaggttc tcggatgtga tcacagccgc
     9601 cagcggaatg acaaacgata gaccattcac gccgcccagc ttgctctcat caaccttctt
     9661 gccctgcagc atgttgacgg caatgcgcag accggtcggg gatacaccgg ggtggttggc
     9721 aacagcaatg gagtcgaagt ccggattcat cgtcagggct tcctgccaga gcttcaaata
     9781 ctgg
//
LOCUS       DBNGSX010000253         1571 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1682904, whole genome shotgun sequence.
ACCESSION   DBNGSX010000253 DBNGSX010000000
VERSION     DBNGSX010000253.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1682904"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1571
                     /locus_tag="AC16CB_07715"
     CDS             <1..>1571
                     /locus_tag="AC16CB_07715"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3342393.1"
                     /translation="QHSQAADEYLNYLVIRPGLVDAYVLDIRGDALQAAGRFGEALVD
                     YRGALQAPSFLDSLTIEIKIARAHAAVGDYETALGLYQDIYTRSDNDFVKAQMDLFMG
                     QAYIAQGNLEQAYTVFQDAVNNYPTSLDSYQALLALVEADIPVDERNRGIVDYYAGQY
                     GVAIAAFDRYFQKGGDDPATARYYNGLSLRALGGYQDAVSQWDIIIQNYPDNRFWDDA
                     WEQKAYTQWAFLEQYPEAIQTLLGFVASAPNHSRAGEFLLDAALVAERSGDLAQAVQY
                     FERVAAEYPGYEKATRSLFLAGVTRARLGDYDNASKVFQRLLENATTPEERASAFLWL
                     GKCQLALGDPSAAALSWELAANSDPTGYYSERAHDLLRQRIPFQPPEQYDLSMDLNAE
                     RVEAENWLRVTFNVPQEIDLSTPAALLGDPRLVRGSELWELGMYDQARDELEAVRQSL
                     QADAVGSYLLANYLLELGMYRSAILSARQVLDLAGMSDVETISAPVYFNHIRFGPYFA
                     ELVLPAAQEKNFHPL"
BASE COUNT          339 a          461 c          422 g          349 t
ORIGIN      
        1 cagcatagcc aggccgcaga tgaatacttg aattacctgg tgatccgccc aggtctggtg
       61 gatgcgtacg tcctggacat acgcggcgat gccctgcaag ctgctgggcg ctttggtgag
      121 gcgctggtag actaccgggg cgccctgcaa gcacccagct tcctagacag cctcactatt
      181 gagattaaga tcgcccgggc gcacgctgcg gttggggatt acgagactgc tctgggctta
      241 taccaggata tttacacccg atcagataac gattttgtca aagcacagat ggatctgttc
      301 atggggcagg cgtatattgc ccagggcaat ctcgagcagg catacacggt cttccaggac
      361 gcggtgaaca actaccctac atccctggat tcttatcaag cgcttttggc tctagttgaa
      421 gcagacatcc cagtcgatga acgaaaccgc ggtattgtgg attattacgc tggccaatat
      481 ggtgtcgcca tcgccgcctt tgatcgctat ttccagaaag gcggcgatga ccccgccaca
      541 gcgcgctact ataacgggct aagcctgcgt gccttgggag gataccagga tgccgtttcc
      601 caatgggaca tcatcataca gaattaccca gacaaccgct tttgggatga tgcctgggag
      661 cagaaagcct atacgcagtg ggcattcctg gagcagtatc cagaggctat tcaaaccttg
      721 ctcggctttg tagccagcgc tcccaatcat tctcgtgctg gtgagttcct gctcgatgcc
      781 gctctggtcg ccgagcgttc aggcgacctg gcccaggcgg ttcaatattt tgagcgcgta
      841 gctgccgaat accctggcta tgaaaaggca actcgatctc tcttcctggc tggcgtcacg
      901 cgggcacgcc tgggagatta tgacaacgca tccaaagtct tccagcgcct tttagagaac
      961 gccactacac ctgaggagcg cgcttcggcc ttcctctggc tgggcaaatg ccagctcgca
     1021 cttggcgatc cctccgctgc cgcattgtct tgggaactgg ctgccaattc tgaccctacc
     1081 ggttactaca gcgagcgcgc ccacgacttg ctgcgccagc gcatcccatt ccaaccccca
     1141 gaacaatacg acttgtccat ggacctaaac gcggagcggg tcgaagctga gaactggctg
     1201 cgagttacat ttaacgtccc tcaagagatt gacctatcca ccccggcggc tctcctcggc
     1261 gacccgcgct tggtgcgtgg tagcgagctc tgggaattgg gtatgtacga ccaagctcga
     1321 gatgaactcg aagccgtacg ccaatcactc caggcagacg cggtaggcag ctacctgctg
     1381 gctaattacc tgctcgagtt gggcatgtac cgctcagcaa tcctatcagc caggcaagtg
     1441 ctggacctgg ctggcatgag cgatgtcgag acgataagcg cccccgttta cttcaaccat
     1501 atccgctttg gaccgtactt tgcagagttg gtcttaccag ccgctcaaga gaaaaatttc
     1561 caccccttgt t
//
LOCUS       DBNGSX010000254         2803 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_921586, whole genome shotgun sequence.
ACCESSION   DBNGSX010000254 DBNGSX010000000
VERSION     DBNGSX010000254.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_921586"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="AC16CB_07720"
     CDS             complement(<1..734)
                     /locus_tag="AC16CB_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR3342394.1"
                     /translation="MNNRRYTGKHPSEFGFEAIQYEKKEYRATITINRPQSLNAVNFQ
                     VLSEMNTALKDASWDDGVAVLVLTGAGERAFCTGADLKEQRQFLKRPRDYWKWMGEFI
                     ECHERLRNLGKPSLARLNGIVVGGGNEFNLSCDLAVAADDIYIRQVGTSHGSVALAGA
                     TQFLPLVVGERRAREILLLNEEIPAHKALEWGLVNWVVSRADLDDKVDEIAQKLVDKF
                     PEKTRYTKQQLNFWRDLSWHLTIGHGR"
     gene            complement(1246..>2803)
                     /locus_tag="AC16CB_07725"
     CDS             complement(1246..>2803)
                     /locus_tag="AC16CB_07725"
                     /inference="COORDINATES: protein motif:HMM:NF014757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HIR3342395.1"
                     /translation="AMTLQLTTPYSIRIGIVGAGTMGSGIALVALLADMQVTLYDVSP
                     QVLDRAREYIATHLTRKNKQSNSANLLLTNRLEDFSGSGIVIEAIPENLELKQGLFCQ
                     LDTICPPPAILATNTSTLSVTAIAAATEHPQRVAGMHFFNPAPVMPLIEVVHGAQTAP
                     ETTRTLLTLTEKLNKTPVTVGDTPGFIVNRVARPFYGEALRILGEGVASHEQIDLLVR
                     KGAGFRMGPFQLMDLIGIDVNLSATQSMYEQTFGEPRYRPHPIQTRMVQQNALGRKTR
                     RGFYRYDIEPDVEPKPPQPRKSNGTVLLGHGSWSPGLEEIVRSAGYSVQSATSTSIST
                     PAIGIALSGIKEGLMNQLIELDRIIPEDAPLLCQCADVTLSEAATWIERPERLVGFDG
                     LFFKGGQAVSLIANPSPNPNILSVVEEFMIGLGRIPLWIEDSPGLILPRIICMLSNEA
                     AFAVQEGVAEGEQIDLAMRLGANYPHGPLAWAKELGYAHVVAVLDHLRNEFGEDRYRS
                     AYLLRRWARL"
BASE COUNT          658 a          731 c          725 g          689 t
ORIGIN      
        1 cgcccgtgac cgatggtgag atgccagctc aggtctcgcc aaaagttaag ctgctgctta
       61 gtatagcggg ttttctctgg gaatttatcc accagctttt gggcgatctc atcaacttta
      121 tcatctagat ccgcacgcga caccacccag tttaccaaac cccattccag cgctttgtgg
      181 gcaggtattt cttcgttcag caggagtatc tcccgtgccc tgcgctcgcc cacaaccagg
      241 gggaggaatt gggttgcccc agccagagca acgctcccgt ggcttgtacc tacctgacgg
      301 atgtatatat cgtctgcagc aaccgccaga tcacaactca agttgaattc attgccacct
      361 cccaccacga tcccgttcag gcgggcgagg ctgggcttac ccagattgcg caagcgctca
      421 tggcattcga tgaactcgcc catccatttc caataatccc gtgggcgttt taagaattgg
      481 cgctgctcct tcaggtcggc tccggtacag aaggcgcgct cgcctgcacc tgtgagtacc
      541 aggacggcaa ctccatcgtc ccagctggcg tcttttagag cggtgttcat ctcgctcaac
      601 acctgaaaat tgaccgcgtt tagagactgc gggcggttta tggtgatggt agcgcgatat
      661 tccttcttct catactgtat ggcttcaaag ccaaactcgg agggatgttt tccagtgtaa
      721 cgtcgattat tcatttgaac tcttgaagat attgttatat agcagggcag ccaccgccgc
      781 ccctaaaaca ggtctaacaa agtaaaccca taggttaggt tggagaaatt ggctgtagcc
      841 agcgctcagg tctatcgtga tcgtttttca tgcgtttctc tttagagggg caatgaattg
      901 tcttgatccc aagcattgat ctacgccggg ttctcaaatt atattatcac aaaatgggtg
      961 aatatagaat gaaattaacg agtgaagcgg agataacaat cacataatgg agcgctggat
     1021 gtttccttgt tctcgagagg aaactatttg agacctctcc ctccataggc atccatacgg
     1081 gtgcgccata ttcgccgtta tggcggcgaa gctccttctg agtgtattca cgcttgatta
     1141 cagacaatct cagtaacctt gataatcgaa tgaagcccga tgtgggtcgg cttcgctaaa
     1201 cttgagtgcg ccaaataccc atgcaacgga atatgccaga ttatcctaca agcgcgccca
     1261 tcgtcggagc aggtaggcgc ttcgatagcg gtcttcgcca aattcatttc gcaggtggtc
     1321 aagcactgca acaacatgcg catagccaag ttctttcgcc caggcaagcg gcccgtgtgg
     1381 ataatttgcg cccaggcgca tggccagatc aatttgctcg ccttcggcaa caccttcttg
     1441 aacggcgaaa gcagcttcat ttgagagcat gcagattata cggggcagaa tcaaccctgg
     1501 gctgtcttcg atccacaggg gaatgcgacc gaggccgatc ataaattcct ctaccaccga
     1561 aaggatattt gggttgggag aggggttggc gatcagggaa accgcctgcc ctcctttgaa
     1621 gaatagacca tcgaaaccca ctaatcgttc tggccgttca atccaggtag ctgcttcgct
     1681 caatgtcacg tcagcacact ggcagagcag gggcgcatcc tctggaatga tacgatccag
     1741 ctcgatgagc tggttcatca agccttcttt tatcccgctc aaggcaatac caatagctgg
     1801 cgtggagatt gaggtcgagg tagcgctttg aactgaataa ccagcactac gaacgatttc
     1861 ctcgagcccg ggcgaccagc tgccatgccc taataagacg gtcccatttg atttgcgcgg
     1921 ctgtggaggt ttgggttcta cgtctggctc tatatcatat cggtagaaac ctcgcctggt
     1981 tttcctaccc agcgcatttt gttgtaccat acgggtctgg atgggatgcg ggcgatagcg
     2041 tggctcgccg aaagtctgtt catacatgga ttgcgtggca gaaagattta catcgatgcc
     2101 aattagatcc atcaattgaa agggacccat gcggaatccg gctcccttgc gaaccaaaag
     2161 atcgatctgc tcgtgggaag caacgccttc ccccaatatg cgtaatgcct ccccgtaaaa
     2221 ggggcgcgcc acgcggttga cgataaaacc cggtgtatca ccaaccgtca caggtgtttt
     2281 atttagcttt tcagttaggg tcaacagggt gcgggtcgtc tcgggagcgg tttgagcacc
     2341 atgcaccact tcgatcaatg gcataacagg agctgggttg aaaaagtgca tgcctgccac
     2401 ccgctgcgga tgttccgttg ccgcagcgat ggcggtcacc gatagggtgc tggtgttggt
     2461 tgccaagatg gctggcggcg ggcagatcgt atccagctgg caaaaaagac cttgttttag
     2521 ctccaagttt tccggaatag cctcaatcac tatcccggaa ccggaaaaat cttccagacg
     2581 atttgtcagc aacagattgg ctgaattgga ttgcttgttc ttgcgtgtca ggtgggtagc
     2641 gatatattct cgggcgcgat ccagcacctg tggagaaacg tcgtagagcg ttacctgcat
     2701 atctgccagc agggctacta aagcaatacc gcttcccatg gtgcccgcac caacaatgcc
     2761 aattcgtatg gagtagggag tggtcaattg aagtgtcatt gcc
//
LOCUS       DBNGSX010000255         3250 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_228192, whole genome shotgun sequence.
ACCESSION   DBNGSX010000255 DBNGSX010000000
VERSION     DBNGSX010000255.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_228192"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="AC16CB_07730"
     CDS             complement(<1..314)
                     /locus_tag="AC16CB_07730"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3342396.1"
                     /translation="MAVIVLVVAAIVWAIVSSQKPEESAGQIPELVGTKQYASAPPML
                     IDQSKQYTATVKMVKGGEFVIQLYADKAPITVNSFVFLARQGYFDGITFHRVLEGFMA
                     QGG"
     gene            811..987
                     /locus_tag="AC16CB_07735"
                     /pseudo
     CDS             811..987
                     /locus_tag="AC16CB_07735"
                     /inference="COORDINATES: protein motif:HMM:NF012576.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
     gene            1015..1908
                     /locus_tag="AC16CB_07740"
     CDS             1015..1908
                     /locus_tag="AC16CB_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR3342397.1"
                     /translation="MIRQRSYTLGVVTAGLKYIGPSRTLSGITHAAEGVGYSLLLKEL
                     PHFDANDVIPIFQTLLSRHVDGIIWAVPEVGDNRNWVSKLSLELEIPIVYLAMNSQEN
                     IYVVSINNYLGGQLAISHLLEQGYRHIGHISGPLDWWESRERMAAWRDVLKAADLEAK
                     DEHWAEGNWSSASGAQATERLFNQYPEMDAIFVANDQMALSVMQYACRKGLRIPDDLG
                     IVGFDDIPESAFFWPPLTTVQQNQYNVGEVVVEEIIKIIESYRQEQEPVQPKSIMLAP
                     ILIVRQSSLRMRGVKGGESGR"
     gene            2109..3104
                     /locus_tag="AC16CB_07745"
     CDS             2109..3104
                     /locus_tag="AC16CB_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR3342398.1"
                     /translation="MRVVRLHSQGELRLHDEPEPVPLAGEELLRVKAVGVCGSDLHWF
                     TEGEIGDATLDSPLVLGHEFSAVTGRGERVAVDPAISCGLCEFCQQGYPNLCPQVKFA
                     GHGLQDGALREYMPWPQDHLYRLPDVLSDAEGAMLEPLGVALHATDLGKLRAGMSVGV
                     FGCGPIGLLVVQLARLSGAVQIIATDKLAHRVEAARNYGAHQAIVAGAGQELSEIMAA
                     TGGRGVDIAFEAAGEQDAVDVSVAAVIPGGRVILAGIPGDDHTSFSASIARRKGLTIK
                     LVRRMRHTYPRAIELVSKDLVDLRSLVTHRFPLEKTAEAFAVAQQREGLKVMIEL"
BASE COUNT          783 a          716 c          890 g          861 t
ORIGIN      
        1 ccgccctggg ccatgaaacc ttccaatacc cggtgaaagg taatgccatc aaagtatcct
       61 tggcgcgcca gaaagacaaa actgttcacc gtaatgggcg ctttatctgc gtaaagctgg
      121 atcacaaact caccgccttt taccattttg accgtagcgg tgtattgttt gctctggtcg
      181 atcagcatgg gcggcgcgga agcatattgc ttggtgccca ccagctccgg gatttgccct
      241 gcgctctctt ctggcttctg agaggagaca atagcccaga cgatggctgc cacgaccagc
      301 acaatgacgg ccaaagcgat cacctgaata tttacattcg agttagcctt ggcagagcgt
      361 cttttagatc ttgttgagcg tttcttggtg gatatggtgt tttacccctt cctcgaaatg
      421 ggataccttt gcggatggat aatgtagttg tcgcatcgat cgcactgatg agattagtat
      481 ctcgccatat tataacgaaa atcttctgct tcgctagctg caagttatgc tgttccgtga
      541 tagcacggtt attattagat atgggactta cgcagttgaa tagggaagag ggggagtttg
      601 ggggggcgca gcccgcccaa actcccccat cacctctcga actgcgtaac ttatgagata
      661 tttaccatta gacggagttt agaaaaagcg cttgtcaacc actggcgagg ttgaattgtt
      721 taatctggac ctgattatga attgttgggt ttcccctatg attaaatata tgatatttgt
      781 ctcttgattt cctatttcaa ttttgctata atgttatcga taacgggatc gataacattg
      841 tatcagcgaa ctggacgacc aaccataaag gaagttgcgt ctgcagcggg ggtgtctacg
      901 cagacggtat cccgcgtgat taacaatagg ccagacgttt ccccccaaac tcgaagacgc
      961 gttcaagaaa tcatagatca gttaggctat caacccagtg ccctggctcg tagcttgatc
     1021 cgccagcgca gctacacttt gggggtcgtt actgccggct tgaagtatat aggcccatcg
     1081 cgtactttga gtggcatcac ccatgcggca gaaggagttg gttactcatt gttattaaaa
     1141 gagctcccgc atttcgatgc caatgacgtt attcctattt ttcaaacgtt gctttctcgc
     1201 catgtggacg ggattatctg ggctgtcccc gaagttgggg ataatcgaaa ttgggtcagt
     1261 aagctatctc tggaattaga aatcccaatc gtctacctgg ctatgaattc acaagaaaat
     1321 atctatgtag tatctatcaa caattatctt ggtggacaat tagcaatctc ccatttgctg
     1381 gaacaaggat atcgacacat cgggcatatc tcaggtccgt tagattggtg ggaatctcgt
     1441 gagcgtatgg cggcatggag agatgtttta aaagcagctg atcttgaagc caaggatgaa
     1501 cactgggcgg agggtaattg gtcttcggca agcggcgcgc aagcaaccga acgacttttc
     1561 aatcaatacc ctgagatgga tgcgattttt gttgccaatg atcagatggc tctaagcgtg
     1621 atgcaatatg cttgtcgaaa aggcttgcgg attccggatg atctaggaat tgtcggtttt
     1681 gatgacattc ccgaatcggc ctttttttgg cccccattga caacagtaca gcagaatcaa
     1741 tataacgtcg gtgaagtagt cgtggaagaa attattaaaa tcatagagtc ttaccggcaa
     1801 gaacaagagc cagttcagcc caagtctatt atgttggcac cgattttaat tgtacggcaa
     1861 agttcattaa gaatgagggg ggtcaaagga ggtgaatccg ggagatagtc tcatctccca
     1921 ttgcttaagc ctgctgaatg atatcttttt cgattggttt gtctcggttg atgaaatttc
     1981 aactgttgat tacaagacta ttttggctgg aaccttaggt cataaaaaca ccagtattac
     2041 ggttgtcggt cgcgtctttg tgacgcaccc cagtattagc gaggattttt tcaagagtaa
     2101 caaacgtgat gagagtcgtg cgcctccatt cccagggaga actccgcttg catgatgagc
     2161 ccgagccagt accccttgct ggtgaggaat tgttgcgagt taaagcagta ggcgtatgtg
     2221 gatcagattt gcactggttc accgaagggg agatcggtga tgctacatta gattcccctt
     2281 tggttttggg gcatgagttt tcagcggtca ctggcagagg cgagcgtgtc gccgttgacc
     2341 cagctatctc atgcgggctg tgcgaatttt gccaacaggg atatccaaac ctttgccctc
     2401 aagtgaaatt tgcaggacac ggcttacagg acggcgctct gcgcgaatac atgccatggc
     2461 cgcaggatca tctgtatcgc ctaccagatg tattgagcga tgctgaaggc gccatgttgg
     2521 agcctctcgg tgtagcttta catgcaaccg atttgggtaa attacgagct gggatgagcg
     2581 taggcgtgtt tggatgcgga ccgattggtt tgcttgtcgt gcagttggcg aggctttcgg
     2641 gggcagtgca gattattgct accgataagc tcgcacatcg cgtggaggct gccaggaact
     2701 atggggcgca tcaggcgatt gtggcgggag caggccaaga gttgagtgag attatggcgg
     2761 cgacgggcgg gagaggtgtc gatatcgctt ttgaagcggc cggggagcag gatgcggttg
     2821 atgtgtccgt agcggctgtc atccctggtg gaagggtgat cctggcgggc atacctgggg
     2881 acgaccatac ctcattctca gcatcgattg cccgccgtaa ggggctgaca atcaaactgg
     2941 tgcgccggat gcggcatact tatccgcgtg caattgaatt ggtttcaaaa gatctggtcg
     3001 acctgcgctc gctggtcaca catcgctttc cgcttgagaa gacggctgaa gcatttgccg
     3061 tcgcacagca gcgtgaaggc ctcaaggtca tgattgaact ataggagagc ttatgaaata
     3121 cgcaatcgga attgattttg gtaccgaatc tgggcgagcc gtgctggtgg aagttggttc
     3181 ggggcgcgaa gttacgacgg cggtatactc gtatgcgaac ggcgtcatcg atgaaaggct
     3241 gccggaaagc
//
LOCUS       DBNGSX010000256         6475 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_21413, whole genome shotgun sequence.
ACCESSION   DBNGSX010000256 DBNGSX010000000
VERSION     DBNGSX010000256.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6475)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6475)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6475
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_21413"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="AC16CB_07750"
     CDS             complement(<1..299)
                     /locus_tag="AC16CB_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR3342399.1"
                     /translation="MLPYFSEIFGNTSSVHAYGQQADAALEKARASIAQMLNCLPAEI
                     VFTSGGSESDNMALRGVAFAQRGKRGATHLLISAIEHHAVSRTAEQLAHLHGFEL"
     gene            complement(362..1102)
                     /locus_tag="AC16CB_07755"
     CDS             complement(362..1102)
                     /locus_tag="AC16CB_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002909732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HIR3342400.1"
                     /translation="MNIEYHKWWSPNLGHDMELKVYGHIGKPMLVFPAQGGRFYEYED
                     FKMIEAISEYIEGGKIKVFTVDSLDQQTWANYAAHPVDRARRHEDYDRYITLEVVPFI
                     RKHCGDYGEKMIATGVSMGAYHAGNFFFRHPDIFDTVIALSGLFQLRSFVGDYVDDNV
                     YFNCPLFYLPNLSDPWYLDQYRRSKIIVCVGQGAWEDAMLADAYALKQILEEKHISAL
                     IDIWGHDVNHDWPWWREMMPYFLETLDL"
     gene            complement(1144..2307)
                     /locus_tag="AC16CB_07760"
     CDS             complement(1144..2307)
                     /locus_tag="AC16CB_07760"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR3342401.1"
                     /translation="MNIVFISPHFPPNYDLFCVNLHQLGVNVLGLADEPYELLQPRLK
                     ASLTEYYRVNDMHNYDELLRALGYFTHRYGKIDRIDSQNEYWLETEARLRTDFNIFGL
                     KTDEIGHVKKKSLMKQTFKKAGLEVARGRVVRSISDAKRLIKETGYPVVAKPDIGVGA
                     AKTYKIHNQQELESFFAEKPLVDYIMEEFIGGVICTFDGLTDRDGNMVFYTSHQYSQG
                     VMETVIEDIDIYYYSLREIPKDLEDVGRRVLKAFKVRERFFHFEFFRTEDNRIIALEV
                     NMRPPGGMTTDMFNYACDIDVYREWANIVVHNHFTTDFTRKYHCCYVGRKMKHRYVHS
                     HEHIVGVLGNKLIHHENISGVFSAALGDYGYLIRSQDLNDIYTMADLIQKKAS"
     gene            2755..4173
                     /locus_tag="AC16CB_07765"
     CDS             2755..4173
                     /locus_tag="AC16CB_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HIR3342402.1"
                     /translation="MAFVIKFGTDGWRGQIAEDYTFANVRRCAQGFADYVLAHYDGGE
                     SIVVGYDKRFHSENFASAVAEVLAANGLKVYLTDGATPTPVIAYSIVNKGAVGGVNIT
                     ASHNPATDNGFKVRNEFGGAIAPEGLKEIEGRIPDSEEKVRRVRLDDAISDGRIVRFD
                     PTPAYIEQIHSLIDLQPIKDAGLKVMVDPMWGNGAGWFQNLLGGGKTQILEIHSQRNP
                     LFPEMSRPEPIPPNVNAGLAAAYEQGADALIITDGDADRVGVGDENGQFIDQLRVYAL
                     LAYYLLEVRGERGPIVKTLSTTNMLNKLGKLYNVPVYDVGVGFKYVAPKMLETHAMIG
                     GEESGGYAFHGHVPERDGILAGLYILDMMIKLDKKPSELLDLLFSKIGAHYYDRIDTR
                     FEGDRQSREKMILSASPKAIGGLEVTGLETTDGFKFGLEDGGWLLIRFSGTEPIIRVY
                     TETTHKDRVQDILRDGLRIVGL"
     gene            4177..4974
                     /locus_tag="AC16CB_07770"
     CDS             4177..4974
                     /locus_tag="AC16CB_07770"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR3342403.1"
                     /translation="MFFLADTHCHLDFDTFDSDRDAVIQRAQNVGVGRMLNPGIDIQS
                     SRVALRLAREYDEVYVACGVHPNDALTWDNQSQNELLGMLKQEKVVAVGEIGLDYYRD
                     RAPRDLQKRVLNMQLDLAEKSILPVVLHNRQATEDMMQTLTAWHANLVSSSSPLAERP
                     GVLHSFSGDIDTAKRAVEMNFMIGITGPVTYKNADELRQIIASIPLSSLLIETDAPFL
                     TPHPHRGERNEPAYVRWIAERIAEIHKLSVQVVAEQTSVNSQKVFNW"
     gene            4983..5948
                     /locus_tag="AC16CB_07775"
     CDS             4983..5948
                     /locus_tag="AC16CB_07775"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR3342404.1"
                     /translation="MSRINFLTPIQNNIHLVEERMREQPDGHHPDLYSALEHLLSSGG
                     KRVRPAVALLTGGMYGADPERLVTLGAAIELLHTATLVHDDLIDGALFRRGIATLNAR
                     WSPAATVLTGDYVFARAAKLAADTDSVDVMRLFADTLATIVSGELTQMFTSRGLISRD
                     DYYRRIYAKTASMFELATAAAAVLSPADYETVNSAKQIGNDIGMAFQIVDDVLDFTGE
                     QATVGKPLASDLRQGLVTLPAIYYIESHQDDPDLDSLINNQFYNEEWLDRIVQKIRKS
                     GAIGQAMDEARHFINCALETLRLQPECPERLALEELAQYIIHRSL"
     gene            6184..>6475
                     /locus_tag="AC16CB_07780"
     CDS             6184..>6475
                     /locus_tag="AC16CB_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342405.1"
                     /translation="MSSLAPPNMPVFAVDDPSLVPVSDHFSHMSPIPPSRMFLINKSL
                     KNYIEKNPDSPTFDASQGDGGASLPGVPIQILERAAELQIEHGSAYDMPFGTD"
BASE COUNT         1693 a         1441 c         1571 g         1770 t
ORIGIN      
        1 tcaagctcaa aaccatgtaa gtgggctagc tgctcggcag tccgggacac agcatgatgc
       61 tcgattgcgc tgataagcag gtgagttgca cctcgctttc cacgctgggc gaaggcgaca
      121 cctcgcagtg ccatattgtc actttcggaa cctccagagg tgaagactat ttcagcgggg
      181 aggcagttga gcatctgggc gatgctcgca cgggctttct caagagctgc gtcggcttgt
      241 tgtccataag catggacaga tgatgtgttg ccaaagattt cactgaaata tggcaacatg
      301 gcttccatta cgcggggatc taatggtgtt gttgatgcgt agtccagata aatcatttcg
      361 tttacaaatc taatgtttcc aaaaaatagg gcatcatctc tctccaccaa ggccaatcat
      421 gatttacatc atgcccccag atgtcgatca aagccgagat atgcttttct tcgagaattt
      481 gtttcaaggc gtatgcgtca gcaagcatgg catcttccca tgctccttga ccaacacata
      541 cgataatttt actacgtcgg tactgatcca aataccaggg atctgaaagg ttaggaagat
      601 agaatagggg acagttgaaa tatacattat cgtctacata atcgccaaca aacgagcgta
      661 attgaaataa accgctcaga gcgatgactg tatcaaatat atccggatgg cgaaaaaaga
      721 agttgcctgc atggtaggct cccatgctta cacccgtagc aatcattttt tcaccgtaat
      781 ctccacagtg ctttcgaatg aagggtacta cttcaagggt aatgtatcga tcgtaatctt
      841 catgtctacg cgccctatca acaggatggg cagcgtaatt tgcccaggtc tgctgatcca
      901 ggctatccac tgtgaaaacc tttatctttc ctccttcaat atattcgcta attgcctcga
      961 tcatcttgaa atcttcatat tcgtagaatc gccctccctg agcaggaaaa acgagcatag
     1021 gctttccgat atgaccgtag acctttagct ccatatcatg acccaaattt gggctccacc
     1081 atttgtggta ttcgatgttc atgcactcct catgtgtatg tgatagtcgg aatcgagctt
     1141 cgattatgat gcttttttct gaataagatc agccattgtg tatatgtcgt tgagatcctg
     1201 tgaacgtatc agataaccat aatctcctaa agctgcgctg aagactccac tgatattttc
     1261 atggtggatg agcttattcc ccagaacacc aacaatatgc tcatgagaat gaacatatcg
     1321 gtgtttcatc tttcgaccca catagcaaca gtggtatttc cgcgtgaaat cagtagtgaa
     1381 atgattatgc acaacgatgt tcgcccattc tcggtacacg tcgatatcac aagcgtagtt
     1441 gaacatatct gttgtcatcc ccccaggtgg gcgcatgttc acttctaagg cgataatccg
     1501 gttatcctca gtgcggaaaa actcaaagtg aaaaaagcgt tctcgcactt tgaaagcttt
     1561 taatacacgt cgtccaacat cttccagatc tttgggaatc tcgcgcaaag aatagtagta
     1621 tatatcgata tcctcaatga ccgtttccat aactccctgg ctgtattgat gggaggtata
     1681 aaataccata ttgccatccc gatcggttaa tccgtcaaag gtgcagatca ctccaccgat
     1741 gaactcctcc ataatgtaat ctaccaaagg tttttctgca aagaaggact ctaattcttg
     1801 ctggttgtga attttatatg tttttgctgc acctacaccg atgtcgggct tggctaccac
     1861 cgggtaacct gtctctttga tcaggcgttt tgcatcgctt atcgaacgta caactcgacc
     1921 acgggccact tctaaaccag cttttttaaa ggtttgtttc atcaatgatt ttttcttcac
     1981 atggccaatc tcatcggttt ttagcccaaa gatattaaag tctgtacgta gccgggcctc
     2041 ggtttccagc caatattcgt tctgggagtc aatccgatct attttcccgt aacgatgtgt
     2101 gaagtatccc agcgcacgaa gcaattcatc gtagttgtgc atatcattaa cacgataata
     2161 ctcggtcaag ctagctttga gccggggttg aagtagctcg taaggttcgt cagctaaccc
     2221 caaaacattg acgcctaatt ggtggagatt cacgcaaaat aaatcgtaat tcggtggaaa
     2281 atgaggtgat ataaatacaa tgttcatgat ttgctccaat ctgaaatttg atcatttata
     2341 cgataactct ggtcaaattg caatggaaaa cgcctcaaca acaggtaatt tggttagaag
     2401 ggtcttgaaa acttctagcc ttatcaaata tttagctctc gtgaaggcat cgatctcaaa
     2461 atggatgtgg tcttttagaa aattaatgaa aagtctggac gtgacctgcg agggcgaatg
     2521 cattttatga atatgcaaca atggtcgcaa gcggcccacg tacaaattgg gcaaattttg
     2581 tttggaaaca tgatataaat aggcaaacct tacaggctta ccacgatgga aatttcgtag
     2641 ttgaagggtt agtaccttgc actatgcgcg tcttctagat tattgccctt acctgtccgg
     2701 tttatccctt gatataatac gtttgttatt taactcactt gctgaggagg atggatggcg
     2761 tttgtaatta aatttggaac tgatggctgg cgcggccaga tcgcagagga ttatacattt
     2821 gctaacgttc gacgctgtgc acaggggttt gcggattatg tgttagcgca ttatgacggc
     2881 ggcgaatcga ttgtagtcgg ctacgataag cgctttcact cagaaaattt tgcctcggcg
     2941 gttgcggagg tgctggcggc caatggcctg aaggtatatt taactgatgg agcgacacca
     3001 acgccggtga tagcctattc tattgtcaac aaaggtgcag tgggcggggt caatattaca
     3061 gcatctcata acccagcgac agacaatggt tttaaggtcc ggaacgaatt tgggggcgca
     3121 attgcgccag aaggtcttaa agagattgaa ggtcggatac cagattctga agagaaagtc
     3181 agaagggtga ggttggatga tgccatcagt gacggccgaa tcgttcgctt tgacccaacc
     3241 ccggcgtata tcgagcaaat tcatagcctg attgatctac agccgattaa agacgctggt
     3301 ttgaaggtaa tggtggaccc aatgtggggc aacggagcag gttggtttca aaatctattg
     3361 ggaggaggaa aaacccagat tctcgagata catagtcagc gtaatccttt atttccagag
     3421 atgtcccgtc cagagcctat accaccgaat gtcaacgcgg gcttagcggc tgcgtatgag
     3481 cagggtgctg acgcattgat catcacagat ggcgatgctg atcgagttgg agtaggcgac
     3541 gagaatggac agttcattga ccaactgcgt gtttacgcct tgttagccta ttatttattg
     3601 gaagtgcgcg gtgagcgagg cccgatcgtc aaaacgcttt ccacaactaa tatgctcaac
     3661 aagcttggga agctttacaa tgttccggtc tatgatgttg gtgtaggatt taagtatgta
     3721 gccccgaaaa tgttggaaac tcatgccatg atcggtggtg aggaatcagg cggttacgcc
     3781 ttccatggtc atgtacccga gcgggatgga attctagctg gtctgtacat actggatatg
     3841 atgattaagc tggataagaa gccctctgag ttgttagatt tgctcttcag taaaattggc
     3901 gcacattatt atgaccgaat tgatacgcgt ttcgagggag atcgtcaatc acgtgaaaag
     3961 atgatcttaa gcgcatcccc taaagccatc ggcgggctgg aagtgactgg tttagagaca
     4021 actgatggct tcaaatttgg cctggaagac ggcggttggc tgcttatccg tttttctggg
     4081 acagaaccaa tcatccgtgt ttacaccgag accacgcaca aagatcgcgt gcaagatatt
     4141 ttgagagatg gtctgcgcat cgttgggctg taaacggtgt tctttctagc agatactcat
     4201 tgccatttgg actttgacac attcgatagt gatcgcgatg cagtcatcca gcgagcgcag
     4261 aatgtggggg ttgggcgcat gcttaacccg gggattgata tccagtcgag tagggtcgca
     4321 ttgcgcctag ctcgtgagta tgatgaggtg tatgtagcct gtggcgtgca ccccaatgac
     4381 gcgctaactt gggacaatca atcgcaaaat gagctgttgg gcatgcttaa gcaggaaaag
     4441 gttgtcgctg tcggagaaat cgggctagat tattaccgcg atcgtgcccc aagagatttg
     4501 cagaagcgcg tactgaacat gcaattagac ctggcagaga aatctatttt gccagtggta
     4561 ctgcacaatc gtcaagctac ggaagatatg atgcaaacgc ttactgcctg gcatgcaaac
     4621 ctggtatcat ctagctcgcc tttagcggaa agacccggcg tgttgcactc tttttcagga
     4681 gacattgata cagcgaagcg ggcggttgaa atgaatttta tgataggtat tactggaccg
     4741 gtgacttata aaaacgcaga tgaactgcgt caaatcatcg catctattcc attgtcctcg
     4801 ttattaattg aaacggatgc gccttttttg acaccgcacc cacatcgtgg tgagcgaaat
     4861 gagccagcct atgtgcgctg gatcgctgag agaatcgcag agattcacaa gctttctgtt
     4921 caggttgttg cagaacaaac gtctgtgaat agtcagaaag tctttaattg gtgagtacac
     4981 tcttgagtcg aattaatttt cttaccccaa tacaaaacaa tattcacctg gttgaagaga
     5041 ggatgcggga acagcctgat ggtcaccacc ctgatctgta ttctgcatta gagcaccttc
     5101 tctcttctgg agggaaacgc gttcgtccgg ctgtagctct gctaacgggc ggtatgtatg
     5161 gagctgaccc agagcgcctg gtcactttag gggcagcgat tgaattgttg cacacagcca
     5221 ctctggtgca tgatgacctg atcgatggag cactttttcg ccgagggata gctaccctaa
     5281 acgcgcgctg gtctcccgcg gccacggttt tgaccggcga ctatgtcttc gcgcgcgccg
     5341 ccaagctagc tgcggataca gattcggttg acgtcatgcg cctttttgcc gatacgctgg
     5401 caacaattgt tagcggagaa cttacccaaa tgtttaccag tcgcggtctg atcagccggg
     5461 acgattatta ccgtcgcata tacgccaaaa ccgcctcgat gtttgagctc gcgactgcgg
     5521 cggcggctgt gcttagcccc gctgattacg aaaccgtaaa tagtgcaaag caaattggta
     5581 atgatatcgg tatggctttt caaattgtgg atgatgtgct ggatttcacg ggtgaacagg
     5641 ctacagttgg caaacctctg gcaagtgatt tgcgccaagg tctagttacc ctgccagcga
     5701 tttactacat cgaatctcac caggacgatc cggatctcga ttctttgatt aacaatcaat
     5761 tttataacga ggaatggtta gatcggatcg tacaaaaaat tcgcaagagc ggggcgattg
     5821 ggcaagctat ggatgaagca agacatttta tcaattgtgc tttggagaca ctgcgcttgc
     5881 agccagaatg tcccgaacga ctggctttgg aggagttggc ccagtatatt attcatagat
     5941 cgttatgatt tgcctcttga cgcatcaatt ccaggatgtt atatttccgc tcaacatgtg
     6001 cgcgtcgatg atcactatta ccactaccaa cactacaaat acccgcatgc aaattgcggg
     6061 gtgttggtgt gcgtaaaagg ttgggagcgc atctccaatc tggttagagc gtactgacgc
     6121 cccgtgaaca acggggcgtt ttatttagaa aagatttcat gattttgaag aaaggattgt
     6181 ctaatgtctt ctcttgctcc tccgaatatg cctgtatttg cggttgatga tccatcgctt
     6241 gtgccggtta gcgatcattt ctcgcacatg tcacccatac ctccttcccg aatgttctta
     6301 attaacaaat cgcttaagaa ctatattgag aagaacccgg atagccccac ctttgatgcc
     6361 tcccagggcg acggaggtgc tagcttgcct ggagttccca tccaaatctt ggaaagggca
     6421 gctgaattgc agattgaaca tggctcggcc tatgatatgc cttttggcac cgatc
//
LOCUS       DBNGSX010000257        11033 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_367264, whole genome shotgun sequence.
ACCESSION   DBNGSX010000257 DBNGSX010000000
VERSION     DBNGSX010000257.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11033)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11033)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11033
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_367264"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1499
                     /locus_tag="AC16CB_07785"
     CDS             <1..1499
                     /locus_tag="AC16CB_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342406.1"
                     /translation="ANVLIVLFIFVISTSACGMLGANNALEASAIDDADPQFQDFYRR
                     LGGHQILGPVISRLFTNGQISYQYTVAGLMAYDPLASEHERYYLAPLGLDLGILEAVV
                     QPPDDPSLRYVDGHIIDPQFLSLYQELGAARVTGHPLTEARYNPAKERFEQYFENVGF
                     YRAESDAPGVVHLLAYGQWKCGASCPQPSLSTTEASVEPPIPPGSEFRKTVDRLGQSF
                     TGFAICPPFDAPDGSRQQVFENVVLAIPEEGGGRVILYSTPLSLGIDPEPLVIPRDDP
                     KMFFYPISEGKGYNVPQVFLDYLAQHGGLDAAGPPISEYSLIGNGAYRQCYSNLCLEQ
                     HSQEAGTLRIRPTPLGYKFWQDVVGPAEEGTAIKSGQLEGTEPIDSIQPTPLPTQVIS
                     QPASEGIIVNVWKKYPVIAPGQRQEIGVVVFRDNLPLAGAHMALILTLDNGESVRFEM
                     EPSGSDGQSYYLMEAMDAPLGEPIVYQVCVYYRPGEAACVQDSFLIWQ"
     gene            complement(1486..2073)
                     /locus_tag="AC16CB_07790"
     CDS             complement(1486..2073)
                     /locus_tag="AC16CB_07790"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3342407.1"
                     /translation="MPKDDALRWNSRYLDDERYSTFAQPRAFLVKNAHFLPPKGLALD
                     VAMGLGGNAGFLLSRGLRVVGVDISSVAVHQASKRFAGLMAVVADLANYYLPPETFDV
                     ILNFYYLQRDLWSQYQLALRPGGILYIETLTQEMLQLQPNTDPAYLLAPGELRQAFAS
                     WEILVYQEGWEESRGGRHHPVASLVARKPHTLIAR"
     gene            complement(2141..3334)
                     /locus_tag="AC16CB_07795"
     CDS             complement(2141..3334)
                     /locus_tag="AC16CB_07795"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3342408.1"
                     /translation="MSNSQLSTLLNRYQQLIEISEDLASTLNLETLLNRIVHVAADLC
                     SAEAASILLYNESNHQLHFEAASNLEFPYMRGLMVPVETSIAGWIVTQRQPVIISDAQ
                     QDPRHFKHIGEVTSIQTNSLLGVPMITKDKVIGVLEAINKLSGQFSQEDQELLMALGS
                     QAAVAIENARLFQQSDLISELVHELRTPLTSLNAAAHLLQRPELGDEQRNAILNTLQT
                     EITRLLDMSTAYLDFARLESGRVQYHFKMIDIGALLEECARVIRGKVVEKEINFQLSL
                     LDGSATIHGDNDKLKQVILNLLSNAIKYTPSGGVIKLTGEILDEEIVIHVSDNGIGIP
                     QDSLPFIFEKFYRVPGVEQPIQGTGLGLFICKRIVHDHLGTIEVQSNFGDGTEFILHL
                     PLVKS"
     gene            complement(3327..4103)
                     /locus_tag="AC16CB_07800"
     CDS             complement(3327..4103)
                     /locus_tag="AC16CB_07800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012966062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family NAD-dependent protein deacylase"
                     /protein_id="HIR3342409.1"
                     /translation="MANISEKSQNGITSAADIIKDSHHAVVLTGAGISTPSGIPDFRS
                     QKSGLWQRYDPFEVASLSAFRQKPDKFFEWLHPLAVTIFNAQPNPAHVGIAQLESAGY
                     IKAVITQNIDRLHQRAGSKQVLEVHGSFDTLTCVACFHQVASEPHVQGYLAHAKIPYC
                     PDCGNVLKPDVILFGEQLPVHTWLSAVEASKKCDLMIVAGSSLEVLPVAGLPMRAVEN
                     GAHLIIVNETPTYIDVRADVVFWDDVAEIIPRIVREVLGV"
     gene            complement(4106..5254)
                     /locus_tag="AC16CB_07805"
     CDS             complement(4106..5254)
                     /locus_tag="AC16CB_07805"
                     /inference="COORDINATES: protein motif:HMM:NF018290.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpaE/TadZ family protein"
                     /protein_id="HIR3342410.1"
                     /translation="MAEKILLIDDDIDTLRLVGLMLQRQGFEVQVASNGPQALGMAKA
                     DPPNLIVLDVMMPEMDGYEVARNLRADPATGEIPIIMFTAKGDVDDKVAGFEAGVDDY
                     LTKPTQPRELIAHIRAVLARTAKRTAPLGPADGRAHLIAVLAARGGLGVTSVALNLGI
                     TLREDTKKDVVVAEFRPGQGTIGLELGYQNSESLNRLLKRRPNEISASEIEAELTSHP
                     LGTRLLLSSYQPRDSQLMASVANFEAITRHIAHTARYVIIDLGPALTPVADKVIKLCD
                     EIILVVEPVPQTVTHTKALVNDLFNLGVGEGKITPVLVNRVRSDVQLSWSQVQDQLGL
                     NIAVIFTPAPELAFQASMKNLPIVLQQRDSLTAQQYNKLAERITQRSK"
     gene            5374..6783
                     /gene="tilS"
                     /locus_tag="AC16CB_07810"
     CDS             5374..6783
                     /gene="tilS"
                     /locus_tag="AC16CB_07810"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR3342411.1"
                     /translation="MSDVLHNYCQIDSALPVLLGVSGGPDSVCLLDMLSHLGYRVVVG
                     HFNHRLRDEAFDEARRVEELAAQMQVPFVSDSQDVASYARLKGLSIEEAARELRYGFL
                     FKQAEKLQVGSVMVGHTADDQVETVLMHLLRGAGLDGLKGMAYRSLPNPWSQKIPLVR
                     PLLAFWRQEILDYIDARELQATIDASNLDTTYFRNRLRNEVIPYLEKIQPQLRQLLWR
                     TADIIRHENLLLDGLVEQAWSECVTQQGHGYLALHVASMQGLPLAMRRRLLRTAINNL
                     RPGLRDVGFDIIERGLAFIASPQTKGQCSLVGGLVLLKEEEIVWLSSWEAALPTASWP
                     KMPGSQVIDLHIPGRITLADGWLLQAVEVVASSQVREAIESNSDPFQAWFDADALPEI
                     LQVRNRRPGDRFKPLGMQGHSLKVSDLMVNLKVPQRARAGWPLVCAGEEIIWVPGCRQ
                     AQRGQVHGDSQRLLHLKLG"
     gene            6917..7897
                     /gene="moaA"
                     /locus_tag="AC16CB_07815"
     CDS             6917..7897
                     /gene="moaA"
                     /locus_tag="AC16CB_07815"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIR3342412.1"
                     /translation="MAYDLFGRLIHYLRISLTDHCNLRCVYCMPEDITFRPTAELMQD
                     DEILTLVRVFASVGFDKFRLTGGEPTVRAGVVELVRRIVNTPGVRTLSMTSNGVLFSR
                     LAQPLAEAGLRRVNFSLDTLNPEKFRRLTRRATLAEVWEGIRAAEQAGLTPIKINAVV
                     VRDYSDADAVELARLTYDHPWQVRFIEMMPFAGATEFQRALVVTTEEIKTRLESALGH
                     LELVNGGKLDGEAQLYHLPGARGDIGFISTVSQPFCASCTRARLTADGRLRLCLLRED
                     EVDLLTPLRAGASTEELRQLVLEAIWHKPWGNRLEQGEIPLNRAMSEIGG"
     gene            complement(7933..8823)
                     /locus_tag="AC16CB_07820"
     CDS             complement(7933..8823)
                     /locus_tag="AC16CB_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR3342413.1"
                     /translation="MSPEATSAHITSESLLIPTINAWRIFLDDQGNSVHTVKAFTADL
                     RLLATYLPPDRPLGDITTTDLNTFLEWMQSSRGVPCSPKTLARRITSLKAFFRWLHKN
                     GVILIDPAEKVPQKSVLSPLPEVLTPDEVEAVLDAADKQRRGAKPDARSYLLVSLLLE
                     TGIKKGECIALSPNHLDLEAPSGPHLFVRYASPQHRYKERKIPIPESWVNALHEYKSQ
                     YEIVDRLFPWSPRRLEYLLEDLGEAAGLSKHLSFDMCRWTAALSDWQSGMDHDKIRQK
                     LGISKVQWREISMKLEKLAA"
     gene            9093..9560
                     /locus_tag="AC16CB_07825"
     CDS             9093..9560
                     /locus_tag="AC16CB_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342414.1"
                     /translation="MKHKIVFVTILTLALAMLISSCESAAAPSPTAPVEASPAPAGGV
                     PEYPAPAEGIPEYPAPAQAVPEYPAPQQAMPAYNPYPGPSESASNFIDWTQADALIQS
                     GEVAEVYQAQTLHVTLVLKDGSVALTIEPAIDEVLKSIEQCGEACKDTKVVNQ"
     gene            9643..9978
                     /locus_tag="AC16CB_07830"
     CDS             9643..9978
                     /locus_tag="AC16CB_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006921730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase domain-containing protein"
                     /protein_id="HIR3342415.1"
                     /translation="MGVSAARKRITDLLLSRAGVSLHPHRFGGSEYRLGKRELGHIHG
                     DHLVDIPFSTKVSDEVVSAGQALPHHVLPDSGWVSFYLVESGDEERASFLLRRSCELA
                     LQQHQRRYD"
     gene            10062..>11033
                     /locus_tag="AC16CB_07835"
     CDS             10062..>11033
                     /locus_tag="AC16CB_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342416.1"
                     /translation="MVTVFSAIQTFVLPRGISDPLTRLVFRSIRFVFDLFLKRAQSYA
                     KKDQIMALFAPIGLLALLPTWLLLVTLGYTAMFWATGIQSWVQAFRISGSSLLTLGFA
                     TADHLLQIVLVYSEATLGLMLVALLIAYLPSMYSAFSRRESAVTLLEVRAGNPPSAVE
                     MIKRYHRIHGLDQLGEQFQSWENWFAEVEESHTSLAALVFFRSPRPGRSWVTAAGTVL
                     DAAALTLSAVDTPADPRAQLCIRAGYLALRRIASFFRIPYHPNPHYPEQPISVSRSEF
                     DAAYAELAAQGVPLTANREQAWMDFAGWRVNYDHVLLEMAKITMAPAS"
BASE COUNT         2487 a         2687 c         3026 g         2833 t
ORIGIN      
        1 aggcgaacgt tctaatcgtt cttttcattt tcgtaatctc aacctctgca tgcggaatgt
       61 taggggcaaa caatgcatta gaagcatccg ctatcgatga tgccgatccc caatttcagg
      121 atttttatcg gcgcttgggc ggacatcaga tattgggacc ggtgatttca cgcttgttta
      181 cgaacggtca aatcagctac caatatacag tggcagggtt gatggcttat gatccgctgg
      241 cgtctgaaca tgaacggtat tatctggcgc cgcttggctt ggaccttggc atccttgagg
      301 cagtggtcca acctcccgac gatccttcct tacggtatgt ggacgggcat atcattgatc
      361 ctcagttcct gtcactatat caggaattgg gggcagcccg ggtaaccggt catccgctga
      421 ctgaggcgcg ttataatcct gccaaagagc ggtttgagca gtattttgag aacgtgggat
      481 tttatcgtgc tgaatcagac gcgcccggcg tcgtccattt actcgcctat ggacagtgga
      541 agtgcggagc aagctgtccg caaccctcac tctccacgac ggaagcctcc gttgaaccac
      601 ccattccacc aggttccgag tttcgcaaga cagttgatcg gctgggtcaa agcttcacgg
      661 gcttcgcaat ctgcccacca tttgacgccc ctgatggctc caggcagcag gttttcgaga
      721 acgttgtatt ggcaatccct gaggaagggg gtggtcgcgt aattttatat tccactcctt
      781 tgagcttggg catcgatccg gagccgcttg taattccgag ggacgacccc aaaatgttct
      841 tttatccgat tagcgagggc aaagggtaca atgtgccaca ggtatttttg gattacctgg
      901 cacagcacgg cggtttggac gcagcaggtc caccaataag tgaatattca cttattggga
      961 atggggccta tcggcagtgc tattcaaacc tgtgcttgga gcagcattct caggaagccg
     1021 ggaccttgcg tattcgtcca actccattgg gatacaaatt ctggcaagat gtagttggcc
     1081 cagctgagga agggacggcg attaagtcgg gacagcttga aggaaccgaa ccaatcgact
     1141 ctattcaacc cacacctctt cccacccagg tgatttctca accggcatcc gagggtatta
     1201 tcgtcaatgt atggaaaaag taccctgtga ttgcaccagg gcagaggcag gaaatcggtg
     1261 tggtagtatt ccgtgataac ctccctctgg caggtgccca tatggcgctg atcctgactt
     1321 tagataacgg ggagtcggtc cgctttgaga tggaacccag tggtagtgat gggcagagtt
     1381 actacctaat ggaagctatg gatgccccgc tcggtgagcc aattgtgtac caggtgtgcg
     1441 tttactatcg cccgggcgaa gcggcgtgtg tgcaagacag ctttcttatc tggcaatgag
     1501 cgtatgtggc tttctagcta ccaggctggc aaccgggtga tgccgaccgc ccctggattc
     1561 ctcccagcct tcttggtaaa ccagaatctc ccagcttgcg aatgcctggc gtagttcgcc
     1621 aggggcgagc aggtaagccg ggtctgtatt tggctgtagc tgcagcatct cttgggtgag
     1681 ggtttcaata tagagtatgc cccctggtcg cagagctaat tgatattgcg accataaatc
     1741 cctttgcaag taataaaaat ttaagatgac atcgaaagtt tcagggggca gataataatt
     1801 tgccagatct gcgacaaccg ccatcaggcc ggcaaaacgt ttgctggctt ggtgcactgc
     1861 aacggaggag atatccaccc ccaccacacg cagaccacgg ctgagcagga agcccgcgtt
     1921 gcctcccagc cccatggcga catccaaagc caaacctttg ggtggcaaga aatgagcgtt
     1981 cttcaccaaa aaagcgcggg gttgtgcaaa ggtggaataa cgctcgtcgt ccaggtagcg
     2041 ggagttccaa cggagagcgt catcttttgg catggaaagg atagtttttt agaagaaatt
     2101 cagatcaagg atatttacag agttgttcac ttgtgtgaaa ttatgacttc accaacggca
     2161 gatgcaagat aaactcggtg ccatctccga aattgctttg tacttcaatt gtccccaggt
     2221 ggtcatgaac aatccgttta cagatgaaca gccccaatcc ggtgccctga attggctgct
     2281 ccacaccagg cacccggtag aatttctcaa aaataaaagg gaggctgtcc tgtgggatgc
     2341 ctataccatt gtcgctaaca tggatgacga tttcttcatc cagaatctcc ccggtcagtt
     2401 tgatcactcc tccagagggc gtgtacttga tggcgttgct caacaggttc agtatcactt
     2461 gcttgagttt gtcgttgtca ccatgaattg ttgccgatcc atcaagaaga cttaattgga
     2521 aattgatctc tttttccacg accttccctc gtattacacg agcacattct tccaaaagcg
     2581 cgcctatgtc gatcattttg aaatgatatt ggacacgccc tgactccagg cgggcaaagt
     2641 ccaggtaagc cgttgacata tctagcaatc gggtgatctc ggtttggagt gtgttcagga
     2701 tggcgttgcg ctgctcatcg cctaactccg gtcgctgcaa caggtgtgcg gctgcattca
     2761 aggatgtgag cggtgtgcgc agttcatgta ccagctcgga tattagatcg gattgttgga
     2821 agaggcgggc gttttcgata gcgacagcag cctgtgaacc cagcgccatg aggagttcct
     2881 ggtcttcctg agaaaattgc cctgagagct tgttgatcgc ttcaagcaca ccgattacct
     2941 tatccttggt gatcatcggg acaccaagca aagaattggt ctgtatactg gttacctcgc
     3001 caatgtgctt gaagtgtcta gggtcttgct gggcatcgga aatgatcact ggttggcgtt
     3061 gcgtgacgat ccagcctgcg atgcttgttt caacaggtac catcaggccg cgcatgtagg
     3121 gaaattccaa atttgaggca gcttcaaaat ggagctggtg attggattca ttgtacaaga
     3181 ggatagaagc tgcttcagca gaacacaaat cagccgccac atgaacgatg cggttaagca
     3241 aagtttccag gtttagtgtg gaggccagat cttctgagat ctcaataagc tgttggtaac
     3301 ggttgagcag tgtactcagt tgagagttag acacccaata cctccctaac gatgcgaggg
     3361 atgatctcgg caacgtcgtc ccaaaatacc acatcggcgc gtacgtcgat atatgttggg
     3421 gtttcattga cgatgattaa gtgcgcccca ttttcaacgg cgcgcatggg cagcccggct
     3481 accggtaaga cctcgagcga ggaaccagcg acaatcatta gatcgcactt tttactcgcc
     3541 tccactgcac ttagccatgt gtgtaccggg agttgctcgc cgaacaggat gacatccggt
     3601 ttaagtacgt tgccacaatc tgggcaatag ggaatttttg cgtgggccag ataaccttgc
     3661 acatggggtt ccgacgcaac ttggtgaaag caagccacgc aggtcagcgt atcgaacgag
     3721 ccgtgcacct ccagaacttg cttggaacca gcgcgctggt gcaagcgatc gatgttctgg
     3781 gtaataactg cttttatgta gccagcgctc tccaactggg ctatcccgac atgtgccgga
     3841 ttcggttgag cgttgaatat tgtgactgct aatggatgca gccactcgaa gaacttgtct
     3901 ggtttttgcc gaaacgcaga taatgaagcc acctcgaaag gatcatagcg ttgccacaag
     3961 ccactcttct gcgatcggaa atcaggtatt cccgatgggg tggaaatgcc tgcaccagtc
     4021 agaacaaccg catgatgact gtctttaatg atgtccgccg cgctagttat accgttttgg
     4081 cttttctcgg aaatatttgc catcttcact tgctgcgctg cgtgattctc tccgcaagct
     4141 tgttatactg ctgcgctgta aggctgtctc gctgctgcaa gacaatggga aggtttttca
     4201 tcgaagcctg aaaggctaac tctggcgcag gggtgaatat cactgcgata tttagcccta
     4261 attgatcttg tacctggctc caggagagct ggacatctga acgcacgcgg ttaaccagga
     4321 ctggtgtgat cttgccctcg cctactccaa gattgaacag gtcgttgacc aacgctttgg
     4381 tatgggtaac agtctggggt acaggttcta caaccagaat tatctcgtca caaagcttaa
     4441 tgactttatc agcgactggc gtcaaagcag ggcctaaatc gatgatcaca tagcgggcgg
     4501 tatgggcgat gtgccgtgtg atggcttcga agttcgccac tgaggccatg agctggctgt
     4561 cacgcggttg gtaggaggag agcagcaggc gcgtacccaa tggatgtgag gttaactctg
     4621 cctcaatctc gcttgcgcta atttcatttg gcctgcgttt caacaggcgg tttaagcttt
     4681 cggaattctg gtagcctaat tctaggccaa ttgtgccctg accaggacgg aattcggcca
     4741 cgaccacatc tttctttgtg tcttcacgca gcgtgatacc caagtttaag gcaacgctgg
     4801 tcactcctaa accacctctg gctgcaagca ctgcgatcaa atgggctcta ccatcagctg
     4861 ggccaagggg agcggtgcgt ttggcggtgc gcgccagcac agcacggata tgggcgatta
     4921 actcgcgagg ctgtgtgggt ttggtaaggt aatcatctac gccagcctca aatccggcca
     4981 ctttatcatc gacatcaccc ttggcggtga acatgatgat cgggatttcc ccagtagcag
     5041 ggtcagctcg aaggttgcgc gctacttcat agccgtccat ttctggcatc atcacatcca
     5101 aaacgatcag attgggcgga tcagccttag ccattccaag cgcctgcgga ccattgctgg
     5161 caacttggac ttcgaaaccc tgtctctgaa gcatcaatcc gaccaggcgt aatgtgtcta
     5221 tgtcgtcgtc tataagcaga atcttttcag ccatttgatc ttccttccta catgtgcgtc
     5281 aagtgtcagt ctagcataaa ggcaaggtca aagcaagagg ctttcgaatt tattagcttg
     5341 aatagaatat aattcttaat catgttggca cgtatgtccg atgtactgca caattactgc
     5401 cagatcgatt ccgctctgcc tgtattactg ggtgtgtctg gtggaccgga tagtgtgtgc
     5461 ttgctggata tgctttccca cctcggatat cgggttgtgg taggacactt caaccaccgt
     5521 ttaagggatg aagccttcga tgaggctcgt cgcgtggagg aattggctgc tcaaatgcag
     5581 gtgccctttg tgtcggatag ccaagatgtt gccagctatg cccgattgaa gggtctctcg
     5641 atagaggaag ctgcccggga gctgcgctat ggtttcctgt ttaagcaagc agaaaaattg
     5701 caggtagggt ctgtgatggt cggtcacact gcagacgacc aggtggagac ggtgttgatg
     5761 cacctgctac gcggcgcggg attggatggg cttaagggca tggcttaccg atctttgcca
     5821 aatccctgga gtcaaaaaat ccccttggta cgtccgctgc tggctttttg gcggcaagaa
     5881 atcttggatt acatcgatgc gcgcgaactg caagcgacga ttgatgccag caatttggat
     5941 acaacctatt ttcgcaatcg ccttcgtaat gaagtcattc cctacctgga aaaaatccaa
     6001 ccgcaattgc gccaattatt gtggcgaact gcagatatca ttcgtcatga aaacctgttg
     6061 ttggatggct tggttgagca agcctggagt gaatgtgtga cccagcaggg acatgggtac
     6121 ttagccctac atgtggcctc tatgcagggg ctgcctctgg caatgcggcg gcgtttattg
     6181 cgaactgcga tcaataattt gcggccaggt ttgcgtgatg tgggcttcga tatcatcgag
     6241 cgcgggctgg ctttcatcgc ctcaccccag acgaagggac aatgcagcct ggttggaggg
     6301 ttagtactct tgaaagagga ggaaatcgtc tggctgtctt cctgggaagc agcgctgccc
     6361 acagcgagct ggcccaaaat gccaggctcg caagtgatag atctgcacat acccggcagg
     6421 atcactctcg cagatgggtg gctacttcaa gcggtagagg ttgtggcaag tagccaggtc
     6481 agagaggcga ttgagagcaa tagcgatcca tttcaggctt ggtttgatgc ggatgcgctt
     6541 ccagagatat tgcaggtgcg aaaccgccgg cccggagatc gttttaagcc tttggggatg
     6601 caagggcact ctctgaaggt gtcggacctg atggtaaatc tcaaggtacc ccaacgcgct
     6661 cgcgcaggct ggccactagt atgcgccgga gaagagatta tctgggtgcc aggttgccgc
     6721 caggcgcagc gaggccaggt tcatggagac tcccagcgtc tgttacattt aaaattggga
     6781 tagcagaata ttactatcgg aatttcggca aattgaggag agtggggaga tgaacaagct
     6841 tcgctcaccc aactccccta cttcatccta attacgtgat ttatagttat aatgtttctg
     6901 tttcaaggtg atttttatgg cgtatgatct ctttggccgc cttatccact atctgcgtat
     6961 cagcctgact gatcactgca acctgcgttg tgtttactgt atgccggagg atatcacctt
     7021 ccggcctact gctgagctca tgcaggatga tgaaattctc acgctggtgc gcgtttttgc
     7081 ctcagtagga tttgataaat ttcgcctgac cggcggcgag ccaaccgtgc gggctggtgt
     7141 tgtggagctc gtacgacgca ttgttaatac gcctggtgtg cggacgttat cgatgacctc
     7201 taatggggtt ttgttctcta ggctcgctca acccttagcg gaagcgggcc ttagacgggt
     7261 gaacttcagc ctggataccc tcaacccaga gaaatttcgc cgcttaactc gtcgagccac
     7321 attggcagag gtctgggaag gcatccgggc tgccgaacag gctggactga cccccataaa
     7381 aataaatgct gtggtggtgc gcgattatag cgatgccgac gcagtggaac tggctcgcct
     7441 gacttacgat catccctggc aggtacgctt catcgagatg atgccttttg ctggggcaac
     7501 agagtttcag cgggcattgg tggtcactac tgaagagatc aagacccgct tggaaagcgc
     7561 gcttggtcat ctggagctgg tgaacggtgg caaattggat ggtgaagcac aactttatca
     7621 tttgccgggg gcgcgtggcg atatcggttt tatctccacg gtgagccagc cattttgtgc
     7681 cagttgcacg cgggctcgcc taaccgccga tggcaggctg cgcctgtgtc ttttgagaga
     7741 agatgaagtg gatttgctca ctccgctgcg cgccggggct agtactgaag aactgcgtca
     7801 gctagtcctc gaggcaattt ggcacaagcc gtggggcaat cgtctggaac aaggtgagat
     7861 ccccttaaac cgcgccatga gtgaaatcgg tggataaaac aatccggcgg gcttgcccgc
     7921 cggattgttt tatcaggctg ccagtttttc aagtttcatg ctaatttcgc gccattgcac
     7981 cttggatatt cccagttttt ggcggatctt atcgtgatcc ataccggatt gccagtcgct
     8041 caaggcggcg gtccagcggc acatatcgaa agataggtgt ttgctcaagc cggctgcctc
     8101 tcccaggtct tcgagcaggt actccagccg gcgcggcgac caggggaata gtcgatcgac
     8161 gatctcgtac tgagatttat attcatgtag ggcatttacc cagctctctg gaataggtat
     8221 tttacgttcc ttataccggt gctgggggct ggcgtagcgc acaaacagat gcggaccaga
     8281 aggggcttcc aggtcaaggt ggttgggact tagggcaata cattcacctt tcttgatccc
     8341 ggtttccaat aacagagaaa ccaggagata agaacgagca tcaggctttg cgccgcgccg
     8401 ctgcttgtca gcggcatcca agaccgcctc cacctcatcg ggtgtgagta cttctggcag
     8461 cgggctgagc acagattttt gcggcacttt ttcagcagga tctatcagaa tgacgccgtt
     8521 tttatgtagc cagcgaaaaa aagcttttaa ggaggtgata cggcgggcaa gggtctttgg
     8581 gctgcagggc acgcctcgcg atgattgcat ccattccagg aaggtgttta ggtcagtggt
     8641 tgtgatatcc cccagagggc gatcgggggg taggtacgtg gccagcaagc gcaagtctgc
     8701 ggtgaacgct ttaaccgtat gtacggagtt gccctggtca tccaggaaga tgcgccaggc
     8761 gttgatggtt ggtatgagca gggattcgga ggtaatatga gcagaagtag cttcaggtga
     8821 catgatcact tccaggtgga ttagattttg ggtcgccagt tatgtttgta cgtaagtatg
     8881 gctttataaa tttttcgtgt agtcgaaatt tcgtatagtt taatatgatt tagttgattt
     8941 gtcaactttt cgggtaaaat gctttatata cttcaaaatt atgcagtaag aggagatagg
     9001 ataatttttg agagcgcgag ctgcccaaat taccctcatt taccctgcag ctgcatatgt
     9061 cctatacttt acatttcgtg caggaggact caatgaagca taaaattgtg tttgtcacca
     9121 ttctgaccct ggcattagca atgcttatat catcgtgcga gagcgccgct gccccctctc
     9181 ctactgctcc cgtggaagcc tctccggcgc ctgctggagg tgttccagaa tatccagcgc
     9241 ctgcggaagg cattccggaa tatccagcac ctgcgcaggc tgttccggag tatccggcgc
     9301 cgcagcaggc gatgcccgct tacaatccat atcctggccc aagcgaaagc gccagcaatt
     9361 tcatcgactg gacccaggca gatgcattaa ttcagagcgg agaagttgcc gaggtatatc
     9421 aagcccaaac gctgcatgtc accctggtgt tgaaagatgg cagcgttgcg ctcaccatag
     9481 agcctgcaat agacgaagtg ttgaaatcta ttgagcaatg cggggaagct tgcaaggata
     9541 caaaggtcgt caatcaatag gcaaaatgaa ataatttcgt tcgcctcgaa gtagaatttt
     9601 ctgcctgcat tagcagattc attttggtat tgatgtttgt tgatgggtgt atccgcagca
     9661 agaaagcgca taactgatct cttgttgagt cgggcgggag tgagcctaca ccctcatcgt
     9721 tttgggggga gtgaataccg actgggaaaa cgagagctcg gacatattca tggcgatcac
     9781 ctggtggata tcccgttttc aaccaaagtg agtgatgaag ttgtatccgc tggacaagcc
     9841 ttaccccatc atgtccttcc tgatagcggt tgggttagct tttaccttgt cgaatctggc
     9901 gacgaggaga gggcgagctt cctgttgagg cgttcgtgtg agctagcgct tcaacagcac
     9961 caacggcgat atgattgatt tggtgggatc tcaatgcgag gtgtaagctg gtaggttttg
    10021 ttattcgttc tattgttttt gtgcttggcg cttttctggt tgtggtaaca gttttttcgg
    10081 caatccagac gttcgtttta ccacgcggca tcagcgatcc gttgacccgc ctggtcttcc
    10141 gcagcattcg ttttgttttc gacctattct tgaagcgagc gcaatcttac gccaagaaag
    10201 accagatcat ggctttattt gcccccattg ggctgctggc gttgctgccg acctggcttt
    10261 tattggtgac actgggctac acggcgatgt tttgggctac tggcatccaa tcatgggtgc
    10321 aagctttcag gattagtgga tcatcgctgc tcacattagg gtttgccaca gcagatcatt
    10381 tgcttcaaat tgttttggtg tactctgaag ctacgcttgg cttgatgttg gtagccttgc
    10441 tgatcgccta cttgcctagt atgtacagcg ccttctcgcg aagagaatcc gcggtcacgc
    10501 ttctcgaggt gcgtgcgggt aatccacctt ctgcggtgga gatgatcaag cgctaccatc
    10561 gcattcacgg attggatcag ctgggggagc aatttcaatc ctgggagaac tggttcgctg
    10621 aggtggagga gagccacacg tcgttagctg cactggtttt ttttcgctca ccgcgccctg
    10681 ggcgctcttg ggtgacggct gcggggactg tgctggatgc ggccgcgttg accctttcgg
    10741 cggtagatac ccccgcagac ccgcgcgctc aactttgcat tcgcgctggc tacctggcct
    10801 tgcgcagaat cgcttctttt tttcgtatcc cttatcaccc taacccgcac tacccagagc
    10861 aacccatcag cgtcagtcgg tcggagtttg acgctgccta tgctgagttg gctgcgcagg
    10921 gggtgcccct cactgcgaac cgtgaacagg catggatgga ttttgccggc tggcgggtga
    10981 attatgacca tgtattgctg gagatggcga agatcactat ggctcccgcc tct
//
LOCUS       DBNGSX010000258         8117 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_506119, whole genome shotgun sequence.
ACCESSION   DBNGSX010000258 DBNGSX010000000
VERSION     DBNGSX010000258.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8117)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8117)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8117
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_506119"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="AC16CB_07840"
     CDS             <1..231
                     /locus_tag="AC16CB_07840"
                     /inference="COORDINATES: protein motif:HMM:NF019844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR3342417.1"
                     /translation="MPMGDTNVYASPDTSAAVLFVLHLGEFAAVLGTSAGGDWAKVDL
                     GPGNTGSNAVGWVEAASLNMNGPCGSLPTISP"
     gene            433..609
                     /locus_tag="AC16CB_07845"
     CDS             433..609
                     /locus_tag="AC16CB_07845"
                     /inference="COORDINATES: protein motif:HMM:NF018096.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HIR3342418.1"
                     /translation="MTKVVHCRDLGFDCEGVVRAESAEEALQMVAQHAKEVHGMEEVP
                     AEVVEKVHEVMQEE"
     gene            1174..2487
                     /locus_tag="AC16CB_07850"
     CDS             1174..2487
                     /locus_tag="AC16CB_07850"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HIR3342419.1"
                     /translation="MSQYDFEKFFELSLDMLCIAGTDGYFKRVNPSFQRVLGWTPEEL
                     TGRPFIDFVHPDDIEATLKEVEKLASRIPTISFRNRYQCSNGYYRHLLWTAFPEKETG
                     LFFAIAHDTTELVEANQRFQLAVDASPVSQIMVDQHGLIQLINRETERLFGYSRDLLI
                     GKSIEMLVPATIDEQHQRDRSAFFQNPGTRLMGEGRHVRAMAQDGREFPAEIGLNPVQ
                     FSDGVYVLCTVIDLTFQKKSEERMMQLAKELEEANAQLSQLAMTDRLTSVFNRHAFDE
                     QLDHLTQLMQRMGKPLSLLMIDIDHFKRYNDRYGHPMGDEALKTVASLLCQNARTSDV
                     VARYGGEEFAIIMPNTAEEEAMQLSDRLRKIFHAHEWEKEMLTISIGASTIFHGKTVD
                     ETKSKKSKYDARLIIDADQALYYSKNNGRDRATHVSEIPDTDHQT"
     gene            2685..3167
                     /locus_tag="AC16CB_07855"
     CDS             2685..3167
                     /locus_tag="AC16CB_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009780434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HIR3342420.1"
                     /translation="MDPVNGYSLELVIGDYEERFCPMTELKTQKNDASVEDFLNAVPD
                     EKKRRDCFAVLELMGDITGERPAMWGTSIVGFGSYHYKYASGHEGDWFLTGFSPRVQN
                     LTLYIMSGFDNYEALLADLGKYKTGKSCLYINKLEDIDLRTLRKLIRQSVAHVAETNK
                     "
     gene            3425..3733
                     /locus_tag="AC16CB_07860"
     CDS             3425..3733
                     /locus_tag="AC16CB_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342421.1"
                     /translation="MRQKQPPKPQKKMDQRQQVQQELERQQEQSQKLRQEEQQELEQQ
                     QEQRRKLRQEEQQELERQQEQKRKLRRQEQQKLEQQQEQERKLRQKEQQKLEQQQRRK
                     "
     gene            complement(3996..4898)
                     /gene="rarD"
                     /locus_tag="AC16CB_07865"
     CDS             complement(3996..4898)
                     /gene="rarD"
                     /locus_tag="AC16CB_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HIR3342422.1"
                     /translation="MSNKGILYGIGAYLMWGFFPIYFKAIHTVPALQIMFHRVVWCFI
                     FLAILILLKKEWGKLKAQVTKPKTLVIYTLAASLLAVNWLVYIYGVNSDQIVETSLGY
                     FINPLLNVALGVIVLREKLRPAQWVPIGLAAAGVTYLTMQHGALPWIALALAFSFGLY
                     GLVKKVSPLGSLYGLTLETAILFLPGLAYLLFAETQGSGSFAHAGWQVTLLLALTGVV
                     TALPLLLFASAARSIPLYMVGILQFIAPTCQFLLGVFLYQEPFSQIQLVGFSLIWSAL
                     LFYWLEGVLERRKLILRQTQIKTS"
     gene            5115..5531
                     /locus_tag="AC16CB_07870"
     CDS             5115..5531
                     /locus_tag="AC16CB_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342423.1"
                     /translation="MNKRFPHIMIVLTLVAVLAGALAFSTSTAKAENGTFNVSVYHDI
                     NGRNLGLSRELPVIATVSSTDGLYAVVPLKFKDRFSADLPAGTYTIMVSAVINGSLVE
                     LPSMSVGPVTIPADVDVRLIARLKSDGTPYIKVRVQ"
     gene            5752..6573
                     /locus_tag="AC16CB_07875"
     CDS             5752..6573
                     /locus_tag="AC16CB_07875"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3342424.1"
                     /translation="MLNILKDLSAPSLARSVKANLYAFFHILGRSSKSEFYTHPKLVR
                     WHTPIPHPWFQGILSAESAELDDDQLVRDMASYFKSRGVTSFTWWLEPNLRPDSWSSH
                     LSAHGFRYDKSTPGMAVHLAALPGSVPQPEKLVIRVVDDLEALEVWVRSFMLGYGLPE
                     AWSIDFFELLASLGLDLPIRHYLAYLDGEPVANSTLLLGAGVAGIYNVATIPEARGQG
                     IGAALTLFPLQQAAELGYHAGILQSSEMGFSVYRRLGFQHLCQMDHFFWKSDVDS"
     gene            6703..7668
                     /locus_tag="AC16CB_07880"
     CDS             6703..7668
                     /locus_tag="AC16CB_07880"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HIR3342425.1"
                     /translation="MTNAKPWKYRKVSIVGAGAVGSTFAYALAQSGLASEIAIIDRNQ
                     ELARGQVLDLLHGVPFFPSVEIHAGEQRDYADSQVIVITAGSKQSQGETRLDLLQRNA
                     SIIEAIVDDILHERSQAILVIVSNPVDVLTYVALKRSGWDKSRVIGSGTVLDSARFRY
                     ALSRHCDVDVRNVHAYILGEHGDSEVAAWSMTHIAGVPIDDYCPFCKRCGNWEEEKSR
                     IVEEVRNSAYHIIDYKGATFYAIGLALVSIVGAILRNQRSVLTVSNLLDGEYGMKDVC
                     LSVPCVLSERGIEHIIEAKLMPHEQEALSNSGSILRSALLELGHG"
     gene            7690..>8117
                     /locus_tag="AC16CB_07885"
     CDS             7690..>8117
                     /locus_tag="AC16CB_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019062982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HIR3342426.1"
                     /translation="MEDARTQAKRVLDRIVLSETTVSLGTAILVGAGTGIGAVLFIRL
                     IELVQKIFFEGSELALGSLGRGLILLIPIFGGLLAGPIIAFFASEAKGHGVPEVMQAI
                     ALRGGRIRPRVVVAKILASALCIGSGGSAGREGPIVQVGAA"
BASE COUNT         2108 a         1875 c         2170 g         1964 t
ORIGIN      
        1 atgccgatgg gcgataccaa cgtctatgcc agcccggata cgagcgctgc ggttctcttc
       61 gtgctgcacc tcggggaatt cgccgcagtg ctgggcacga gcgcgggcgg cgactgggcc
      121 aaggtggacc tgggaccggg caacaccggc tcaaacgctg taggctgggt ggaagccgcg
      181 tctttgaaca tgaatggccc ttgtggcagc ttgccgacca tcagcccgtg aaccttatct
      241 gatttaacgg ttattgcgag taaccacagc gttagcattt ttcttgcgtg tggaaaccgt
      301 ctttgcgagg gaaccctgcc ctggcacttg cacccagggc aagcgggcgt tagatgctat
      361 cttatcgcga gggcaggttt aagtattcac gctagaataa aatatgtagc gtatgtcaaa
      421 aggaggctaa aaatgacgaa agttgtgcat tgtagggatc tggggtttga ctgcgagggc
      481 gtagtgcgag ctgagagcgc agaggaagcc ctgcagatgg ttgcccaaca tgccaaagag
      541 gtgcatggca tggaagaggt tcccgctgag gtcgtggaga aagtccacga ggtcatgcag
      601 gaagagtaag ccacccacta aacattacgc cactccatcg acgcctacaa tttcataagt
      661 gatttacgtc cgcagtgcat acatgctgac aatatactca atggaacggg cgcaggaatc
      721 gcgcggctgt actatgttta ccggttcata ttgaaggcat ggctggcatg aggaaatcgt
      781 agaggcttaa gctattttcc gtctcggatc cctccgaatg cggtggagga gagtataagc
      841 gataattagg tgtactgtgg ggatgctgcc caactctcgt tggtttaaac attgctccat
      901 aaagtggctg acactcacaa tatgcctaat cagatagtat tgtagcagca ttgaaattat
      961 ctggtaggat tagatctgaa ctgcttgtat acgctcatcc ctcttgctaa taaaattgtc
     1021 aacgaattcg ctaagatagt taatcaggta tgaaatactg taaagcatta ataggacctt
     1081 tcactctcgg tatgatggag tggtggcaag ctgatgaatt ttgtcggtgg ttgccattaa
     1141 tatttcatca ggcaaagatc aaggagaaat tcaatgtctc aatatgactt tgaaaaattc
     1201 ttcgagctct cgctggatat gctttgcatt gctggcaccg atggctattt caaacgcgtc
     1261 aacccttcat ttcaacgcgt tcttggctgg acacctgaag aattaaccgg ccggcctttt
     1321 attgactttg tccatcccga cgacattgag gcaacattga aggaagtgga aaagctggca
     1381 tcccggatcc ccactatatc atttcgaaat cgctatcaat gttcaaatgg gtattaccga
     1441 catctcctgt ggacggcttt tccggaaaaa gaaacgggat tgttttttgc gattgcacat
     1501 gacacgactg agttggtcga ggcgaatcag cgtttccaac tggcagttga cgcttcgcct
     1561 gtatcccaga tcatggttga tcagcatggc ctcatacaac tcataaacag ggaaacggaa
     1621 cgtctctttg gttattcacg ggacctcttg atcgggaaat cgattgaaat gctggtccca
     1681 gccactattg atgagcagca tcagcgggat cgatccgctt tttttcagaa ccctggtacg
     1741 cgtttgatgg gtgagggccg gcatgttcgg gcgatggctc aagatgggag agaatttcca
     1801 gccgagattg gactcaaccc cgttcaattt agtgatggag tatacgttct ctgcacggtc
     1861 attgatctga catttcaaaa aaagtcggaa gaaagaatga tgcaattagc aaaagaactt
     1921 gaggaggcaa atgcacaact gtcacagctg gcaatgactg acaggctgac cagcgtgttc
     1981 aatcgacacg catttgatga gcaacttgac catctcaccc aactcatgca gcggatgggt
     2041 aaacccctct ccctcttgat gatcgatatc gaccatttca aacgatataa tgatcgatat
     2101 ggacatccaa tgggcgatga ggctttgaag accgtagcca gtctgttgtg tcagaatgcg
     2161 cgcaccagtg atgtcgtagc gaggtatgga ggagaagagt tcgcaattat aatgcccaac
     2221 acagccgagg aagaggccat gcagctttct gataggctgc ggaagatctt tcatgctcat
     2281 gagtgggaga aagaaatgct gacgatcagc atcggtgctt ccacgatatt ccatggtaag
     2341 acagtagacg agaccaagag taaaaagagc aaatacgacg cgcggcttat catagacgca
     2401 gatcaagctc tctattattc aaagaataat ggtagagatc gtgctaccca cgtttctgaa
     2461 ataccagaca cagatcatca aacctaacag ctgcttccaa gcgtgtttgc tttcactatg
     2521 ctccggtcac gtagaaaaaa atggcggttt gacagtatcc attgactaat tgatgaagtc
     2581 cattaccttt tggcttatga agacctttgc ttagtcggta ttaggagcaa tttggtattt
     2641 cccgccaccg ttgacaactc tctgtctgat gcatggatgg atacgtggat cctgtcaatg
     2701 gttactcctt agagttggtg attggtgatt atgaagaaag gttttgtcca atgaccgaac
     2761 taaagacgca gaagaatgac gccagcgttg aagactttct caatgctgtg cccgatgaaa
     2821 agaagcgccg tgattgtttc gctgtcttag agctcatggg cgatatcaca ggggagcggc
     2881 cggcgatgtg gggaacgagc atcgtcggat ttgggagcta ccattacaaa tacgcctccg
     2941 ggcatgaagg agattggttt ctgaccggtt tctcgcccag ggtgcagaac ttgaccctct
     3001 atattatgtc gggctttgac aactacgagg cgttgctggc ggatcttggc aagtacaaga
     3061 caggcaaatc ctgcctgtat atcaacaaac tggaagatat tgatctccgc accttgcgaa
     3121 agctgatcag gcagtcggtg gcccacgttg ctgaaacaaa taaatagcgc aggtcgtgcc
     3181 tgaccatctg cgggagcagg aatgcagcgc tcattgtaga cgggactagc cccggtagtt
     3241 ccctggcctg aactggtctt cagaccaaac gggaagctca tgaccaaagg cacaagaacg
     3301 aatcgcatag ccatgcagtt agattacaat ataagagcct cacagaaaga tggttgggtc
     3361 gccaacacca gctgtgagtc tgtgtggcgt gcaggtaaga ttagcgctca agaaggaaaa
     3421 caaaatgaga caaaaacaac cgccgaagcc gcagaagaaa atggaccaac ggcagcaagt
     3481 gcagcaagaa ctggagcgac aacaggaaca gtcacaaaaa ctgcggcaag aagagcagca
     3541 agagcttgag cagcaacagg aacagagacg aaaattgcgg caggaagagc agcaagaact
     3601 ggagcgacaa caggaacaga aacgaaaatt gcggcgacag gaacagcaaa aactggagca
     3661 gcaacaggaa caggaacgga aactgcggca aaaggaacag caaaaactag agcagcaaca
     3721 gagacggaaa taacaaaagt tgctgcggag tccctgcgcc tttctcgtct gatgcccagg
     3781 ctggacgcga taatcgtaga gttagagatc ttagagggtt acatagtgta gggcacaccg
     3841 tggggtggtt gcgcgtccaa tacctgccta cgcaggcagg tattgggtat tcgttaataa
     3901 ggaaatttag gctggcgaag gcttggggca gcgctttggg gctgacctgt ttccatggtt
     3961 gaatccattt cgggttggcg gcggatttct tttatttatg aggtttttat ttgtgtttgt
     4021 ctcaagatca gcttgcgacg ctctaaaacc ccctccagcc agtaaaacaa gagagcgctc
     4081 caaatcaggc tgaatcccac caattggatt tgtgaaaatg gctcttggta gaggaacaca
     4141 cccagcagga actggcaggt aggggcgatg aactgcaaga tgcccaccat gtagagcgga
     4201 atcgatcggg ctgccgaggc gaacaagagc agcggcagcg cagtgacaac cccggtcagc
     4261 gccagtaaga gcgtcacctg ccagccagcg tgggcgaagg atccactgcc ttgggtttcc
     4321 gcaaatagga ggtaagccag gccgggcagg aaaagaatag ctgtttccaa cgtcaagcca
     4381 tacagtgacc ctaaggggga gactttcttc accaggccat agagaccaaa gctgaaggcc
     4441 agcgccaggg cgatccacgg cagggctccg tgctgcatgg tgagataggt caccccggca
     4501 gccgctagcc cgatgggcac ccactgcgcc ggacgcagct tttcacgcag gacgatgacc
     4561 cctaatgcca cgttcaacaa gggattgata aagtaaccca ggctggtctc tacaatctgg
     4621 tcgctgttga ctccgtagat gtacaccagc cagttaaccg ccagcaggct ggctgccagg
     4681 gtatagatga ccagggtttt cggcttggtg acctgcgctt tcagtttccc ccattctttt
     4741 ttgagcagga tgagaattgc caggaatata aagcaccaca ccacccgatg gaacatgatc
     4801 tgtagcgccg gcactgtgtg gatggcttta aagtaaatgg ggaagaaccc ccacatcagg
     4861 tatgcgccga taccatataa gatacccttg ttggacatcc taacctctgg gattagagta
     4921 aataacaggt tcaatgatac caccgtcttt gctctcgatc atggctggat ccgggatctc
     4981 atctcaaaat gtgtatatat aatgtatatt attaatcaaa tacttaatta ataatattga
     5041 cattttgtat aattagtgta taatattgat aacttaatct tcacaaaaca tatacacaac
     5101 gaaaggaatt tacgatgaac aaacgctttc cccacattat gattgtccta actttggtag
     5161 ctgtcttggc tggtgctctg gcctttagca cgtcgacagc caaagctgaa aatggcacct
     5221 tcaatgtctc tgtgtaccat gatatcaatg gcaggaacct gggtctctcg agggaattac
     5281 ctgtaatcgc cacggtttcc tccaccgacg gtttatatgc tgtagtccca ttgaagttca
     5341 aagatcgttt ctctgctgat ttgcccgctg gcacctacac gattatggtc tctgcggtca
     5401 taaacggtag cctggttgag ctgcccagca tgagcgttgg cccggttacc atccctgccg
     5461 atgtagatgt gcgcctgatt gccaggctta aatcagatgg caccccctat atcaaagtac
     5521 gtgtgcaata atcatcaagt aatacttttg agattgttgt ccaaaacata actttgcaat
     5581 cgaataggtg ttgtacccaa gccccggagt tcaatccggg gcatggtctt taatttgatt
     5641 atgggtgaaa atatttcttc catctctaga atgggagtaa aaatttatga gtataattcg
     5701 gagaccgacc caccgagatt agaatgttgt aactcgagat cgaatattgc catgttaaat
     5761 atactgaaag atctctccgc cccgagcctc gccagatcag tcaaagccaa cctgtacgcc
     5821 ttcttccata tacttggacg ttcctcaaaa tctgagttct atacacatcc caaattggtg
     5881 cgttggcata ccccgattcc gcacccttgg tttcagggca tcctcagcgc agagtctgct
     5941 gaactggacg acgaccagct ggtccgcgat atggcatcct atttcaaatc acgcggcgtg
     6001 acgagtttta cctggtggtt ggagcccaat ctaaggccgg atagctggtc tagccacctg
     6061 agcgcacatg gctttcgcta cgataaaagc actcctggca tggctgtgca cctggctgct
     6121 ttaccaggct ccgttccaca gcccgagaaa ctcgtaatcc gggtagttga tgatctggag
     6181 gccttagagg tgtgggtacg tagctttatg cttggctacg ggttgcccga agcgtggtcg
     6241 atagactttt ttgaactgct tgccagcctg ggactcgatc ttccaattcg gcattacctt
     6301 gcctatttgg atggcgaacc cgtggcaaac tccacactct tgctcggagc tggtgtggca
     6361 ggcatctata atgttgccac gattcctgaa gcaagggggc aagggatcgg cgcagctctg
     6421 acgttgttcc cgttgcagca agccgctgaa ttaggctatc acgctgggat cctgcaatcg
     6481 tccgagatgg ggttcagcgt atatcggcgt ttgggttttc aacatctctg ccagatggat
     6541 cattttttct ggaagtctga tgtggatagc tgacaggaag tggttgataa taaagacagg
     6601 atttccacaa cgggagatgg gatgaggaag cttggggagg aagccctcca tcgatccatt
     6661 cttcattaga ttgcgcaaat cctgagagag aaggtatttc ttatgaccaa cgcaaagcct
     6721 tggaagtatc gaaaggtttc tatagtcggt gcaggcgccg tgggatccac gtttgcctat
     6781 gccttagcac aaagcggttt agcctccgag atcgctataa tagaccgtaa tcaagagtta
     6841 gcgcgtggac aggtgcttga cctgctgcat ggggtgccat ttttcccttc ggttgagatc
     6901 catgcagggg agcaacgaga ttatgccgat tcgcaagtga tcgtgattac cgcgggcagc
     6961 aaacaaagcc agggggaaac gcgcctggac ctgctccagc gcaatgccag catcattgaa
     7021 gcaatcgttg atgatattct acatgagaga tctcaggcaa tcctggtaat tgtcagcaat
     7081 cccgtggatg tcctgaccta tgtggctttg aaaagatcag gttgggataa aagcagggtc
     7141 attggttcag gcactgtact ggatagcgct cgctttcggt atgcactcag ccgccattgc
     7201 gatgtggatg tgcgcaatgt tcatgcttac attttaggcg agcacgggga tagtgaagta
     7261 gcggcctggt cgatgacgca cattgcaggc gtgccgatcg atgattattg cccgttctgt
     7321 aagcgttgtg gaaattggga ggaggagaaa tccaggatcg tcgaggaggt gcgcaattcc
     7381 gcctaccata ttatcgatta taaaggggcc actttttatg ctatcggatt agccttggtg
     7441 agcattgtgg gggcgatctt acgcaaccag cgcagtgtgc tcaccgtatc aaatcttctg
     7501 gatggggagt atggcatgaa ggacgtgtgc ttgagcgtcc cttgcgtgct ttcggagcga
     7561 gggatcgagc acattattga agccaagctc atgccacacg agcaagaggc gctatccaat
     7621 tcgggatcta ttctccggag cgcattacta gagcttgggc acggttaaat gcccttttgg
     7681 agagaaataa tggaagatgc tcgtacgcag gccaagagag tacttgatcg cattgttcta
     7741 tctgaaacaa ctgtgtcgct cggaacagcc atcctggttg gagcaggcac tgggatcggt
     7801 gcggtcttat tcattcgact gatcgagctt gtacaaaaaa tattcttcga aggcagcgag
     7861 ctagcgcttg gctctctagg tcgaggactg atcctgctta tacccatatt tggtggattg
     7921 ctggctgggc cgattatcgc tttcttcgcc agtgaggcca agggacatgg cgttcccgag
     7981 gtcatgcagg caattgccct gcgcggcggg cgcatccgac cccgagttgt agtggctaaa
     8041 attcttgctt ccgcgttatg catagggagc ggcggatctg ctggtcgcga gggtccaatt
     8101 gtgcaagtcg gcgcggc
//
LOCUS       DBNGSX010000259         2190 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_574746, whole genome shotgun sequence.
ACCESSION   DBNGSX010000259 DBNGSX010000000
VERSION     DBNGSX010000259.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_574746"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="AC16CB_07890"
     CDS             complement(<1..494)
                     /locus_tag="AC16CB_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342427.1"
                     /translation="MIAAVFLIPGWAFLSLTRFWQKWDTLQRWCVAMGLSISFYPVLF
                     YATRSLFPFLHIGPNKNLFLLIAFLVIAIWGFRRNLFDQIKFDPLEWLVIGIWCAIIY
                     TRLMFAWQNPYPAWSDSLHHTLLTQLTAVRGQLPYTLEPYEPTWLGMYHLGLYSLTGL
                     VQQLG"
     gene            complement(649..1467)
                     /locus_tag="AC16CB_07895"
     CDS             complement(649..1467)
                     /locus_tag="AC16CB_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HIR3342428.1"
                     /translation="MDDQIVAVFCLCDDMLKALGHHEDPQCQMSDAEVMTTAITAALR
                     YAGNFEHARKFLRDEGYIPKMLGKSRFNRRLHRIQNLFLTLFRLLGETWKDLNSKSIY
                     VLDSYPVASCDNYRICRSRRYRGEEWRGYQASKRRYFYGLKIHILVAEHGQPIEFFLT
                     PGSYSDTSAYRLYDFDLPEGAYITGDKAYNDYDVEDALLEAGLKMSPLRKKNSKRPIP
                     PWAFYLRSAYRKIVETTGSLIERVLPKSIHSVTPEGFELKIGLFVLACSLNFLW"
     gene            complement(2000..>2190)
                     /locus_tag="AC16CB_07900"
     CDS             complement(2000..>2190)
                     /locus_tag="AC16CB_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342429.1"
                     /translation="PTFSMAFAKSTSAKVTENATVFWPTHADGWDNRQSQIHRGTLIM
                     PRFCLRLYWQKANLQGEC"
BASE COUNT          581 a          534 c          530 g          545 t
ORIGIN      
        1 cctagctgct gaaccaggcc cgttaatgaa taaagtccca ggtgatacat tccgagccag
       61 gttggttcat agggctctaa tgtgtagggc aattgccctc ttacagccgt cagttgggta
      121 agcaaggtgt ggtggagtga atcggaccaa gcaggatagg gattttgcca ggcgaacatc
      181 aggcgagtat agatgatcgc gcaccaaata ccgatgacca gccattccaa tggatcaaat
      241 ttaatttgat cgaacaggtt tcttctaaag ccccaaattg caatcaccag gaatgcaatc
      301 aacaagaaca agtttttatt cggtccgata tgcaggaagg gaaagagcga tcgtgttgca
      361 taaaataaaa cgggataaaa ggatatactt aaccccattg caacgcacca ccgctggagc
      421 gtgtcccatt tttgccagaa tcgcgtcaat gaaaggaatg cccaaccagg gattagaaaa
      481 accgcagcaa tcaccaaagt cagccggata agttccagga agttcatgat cgggggccta
      541 cgctgtatat taacaagggt gtcccagaat gtcaactaga tccagtataa taccgtttac
      601 ttctctacaa tcgattaatc attaatttgc attaattaac ccaagttgct accaaaggaa
      661 gttgagactg caagccagca caaacaaacc gattttcagc tcaaatcctt caggagtaac
      721 tgaatggata gatttcggca gcacccgttc gatcaagctg cccgtggttt cgacgatctt
      781 gcgataagca gatcgcaagt aaaatgccca aggtggtatc ggacgcttcg agtttttctt
      841 gcgcagaggt gacattttca atccggcttc cagtaaagca tcctcgacat catagtcgtt
      901 gtaagccttg tccccagtga tataggcgcc ttctggcaag tcgaaatcgt agagacgata
      961 ggcactggta tcactgtagc tgcctggcgt caggaaaaac tcaatcggtt gtccatgttc
     1021 agcgaccaga atatggatct tcagaccata gaaatagcgc ctcttgctgg cttggtagcc
     1081 tctccactct tctcctcggt agcgtcgaga gcggcagatg cggtaattgt cacaggaggc
     1141 aaccgggtaa ctatccagga catagatgga cttgctgttc agatccttcc aggtctcgcc
     1201 taacagacga aacagcgtca ggaataggtt ttgaatacga tgcaaacggc gattgaagcg
     1261 acttttgccc aacatcttgg gtatgtagcc ctcatctctc agaaacttgc gggcgtgctc
     1321 aaagttccca gcataacgaa gtgcagcggt aatcgcagtg gtcatgactt ctgcatcgct
     1381 catctggcac tgtggatctt cgtgatgtcc caaggctttg agcatatcgt cacacaagca
     1441 aaagactgct acaatttggt cgtccatgtg ttctcctggt ttagtcgtgg tttatgcacc
     1501 aacaacttta cccgaaaaca catggacttt ctcatcagtt caccccaagg tggcaacttg
     1561 ggttaattat ctcgcgggag caaaacgggg gttctggaag tacgtcagca gctcggactc
     1621 gtcccgcatc aagatcatgc cataggtgag cagcatcgag gccactcgcc atcaactggc
     1681 ctcatcccac gccaatagcc gtcaacacca aaggtttggg cttgtcccag tcttgcgcat
     1741 agatgccgtc caaactcaac atccgtaccg gctgccaatt tggagtatgc cccaaatatg
     1801 ggtatctatc ccgcatgccc tgaaggagcc gttataattt gtttgacttt tccctcctct
     1861 tcccataaac ttaaaccatg ctggcccgcg tattttcttg cgctgtgatt gggttagagg
     1921 gggtgatcgt cgaagtcgag gtcgacaccg ggcaagggtt gcccgggatg actatcgtcg
     1981 ggccaaaaaa cctatgcatt tagcattccc cttgcagatt tgctttttgc caatataagc
     2041 gtaaacaaaa ccgaggcatg atgagcgtgc ctcggtggat ttgtgactgt ctattatccc
     2101 aaccgtcagc atgtgttggc caaaagacag tcgcattttc ggtgactttg gcagatgtgc
     2161 tttttgcgaa ggccattgaa aaggtgggtg
//
LOCUS       DBNGSX010000260         5245 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_991377, whole genome shotgun sequence.
ACCESSION   DBNGSX010000260 DBNGSX010000000
VERSION     DBNGSX010000260.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5245)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_991377"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(381..755)
                     /locus_tag="AC16CB_07905"
     CDS             complement(381..755)
                     /locus_tag="AC16CB_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342430.1"
                     /translation="MSFVEVLHARLANTALLYFGILAIWGLWRTIRKQGLDSSFWGAL
                     VIAELLILVQGATGAYLWIIGERPGRWVHILYGVVTALVIPGLYAYTKGESDRRVMVV
                     YTITLLICVGVILRAMSTATGG"
     gene            1148..1705
                     /locus_tag="AC16CB_07910"
     CDS             1148..1705
                     /locus_tag="AC16CB_07910"
                     /EC_number="7.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="QcrA and Rieske domain-containing protein"
                     /protein_id="HIR3342431.1"
                     /translation="MTVSPNEAETDKKQVTRREFLNIAWLASLGFLTVNLGGVTYLFS
                     MPRFKEGEFGGLFSVGRVSELPEAGASPVNFPKVKLWLSNTEEGVMALYKVCTHLGCL
                     YNWNSQEGKFICPCHGSQFDANGEYIQGPAPRSLDRFVVQAVDPNTNQVLAEAADGKP
                     MPVPDQSDAVIRVDTGKRIPGDVHA"
     gene            1750..3420
                     /locus_tag="AC16CB_07915"
     CDS             1750..3420
                     /locus_tag="AC16CB_07915"
                     /inference="COORDINATES: protein motif:HMM:NF012263.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - quinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HIR3342432.1"
                     /translation="MLKSLNFQDLRKKALEAFDNRVRIITAGMGLDELRAVVRGDPPT
                     EKPNPRYKVHTTSFLFHIRPRYYEKGSTIFAHTFRLGFFTSFFFFVELFTGLILMIYY
                     TPSPAEAYNSILELMSNVPFGQVLRDLHRIGAEGMVIFSVLHMLRTFLTGSYKKDRTF
                     TWLTGVFLLGITLFLSFSGYLLPWDQLSYWAVTIGTSMAEAAPLFGTQVNLLLRGAPD
                     IGAGGLLRFYLFHVVLFPLLAIWVISIHYYKVSREHGISLPAKVEEGNVAPEAKKEAK
                     QRIDYIPDLLTHEVFLTALGLFVLVALVTVFNWHAPLEHVANPQQTPLDTKAPWYFWW
                     LQGMLKIDPASILENLFSRVGLQVELSALLNSKQIMGIVLPTILAGLLLGVPYIDRNP
                     HRSLYKRPVAVGFGLLFTLVLVVLSYMGLPEYGIQTPAATRIIQDLAPEEGIGQLREV
                     PFDQLRVGVYEVGDTIPRNLCPDEPAGCPALTGVFAEFSERINSAAKNEHLEPYQRLP
                     NAQAVMLIEDWQHDLRKVTLRILWDDVASGQPKSYERHVFLHRAHGAE"
     gene            3451..4296
                     /locus_tag="AC16CB_07920"
     CDS             3451..4296
                     /locus_tag="AC16CB_07920"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3342433.1"
                     /translation="MSRVQLEISLGILLVLATGTILIYQGLLEEERMARFEEQQRAQA
                     IEVGAELFDINCKGCHGPQGEGTPGLCPPLNDKYFFTDRLKDVGWSGSIEDYIVATVA
                     SGRLTSTRPELYPGNVRPAMPAWSEQYGGPLREDQIRFIAAFVANWEATAPDRQLAPV
                     LAGPPVGTDITQELPAGDAVKGEALASSQGCIGCHVAGPTGPPWNPTADQPGIGDRAA
                     ARLTQSDYSGNAATPEQYLLESIVNPAAYLVPGYETVLMPGTYGQTITAQDAADLIAY
                     LLTVK"
     gene            4304..5146
                     /locus_tag="AC16CB_07925"
     CDS             4304..5146
                     /locus_tag="AC16CB_07925"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3342434.1"
                     /translation="MPVFHRMRSSSLFLFVALVALLSWNFVHAQTSGEEQMEQGASIY
                     AANCAVCHGDDGQGRVGATLAKDWPSIRPDLKVRATIENGIAGSPMPAWSQKNGGPLT
                     DEDIDALVVYILSWETGGPRVFPPTPTYIPRPLISPVPDVVGDPNQGAILYDQNCAVC
                     HGTEGRGRVGASLDKVWPSIRPDLGIKNTISNGVMGSPMPAWSQVNGGPLTETEINNL
                     VAFIMALEPASEEIQQAPTPAPAPGPLADWFGILLAVVLFILVVWIAIVLQTRTQAQN
                     DTEK"
BASE COUNT         1146 a         1414 c         1401 g         1284 t
ORIGIN      
        1 ggcgacaact tcaccacatt cggcacaatt accgccgcgc ggctcgatgg aggcaacgac
       61 ctgctggccc tgatcaacta tctcggcacg ttcagcccgg tatcgccgcc ttccaccggt
      121 agggtcaacg aactgccctg ataccagact ggtgcctgtg gagctgcagt agttagttcc
      181 actattagcg ttgaacgttt cagagtgata aatatcaagg cggactgctt ccctagggaa
      241 gcagtccgca ctcatccatt gccggcatcc tgcaccacca tggtcatcca cattgatggc
      301 aatgctgtga cgcttgcacg cggattcttg atatctggtg attgctgtag gtatgcccca
      361 agtagggctt tttggaatat tcacccaccc gttgcggtgc tcatagcgcg cagaatgaca
      421 cccacacaga tcagcagggt gatggtataa actaccatca cgcgccggtc agattccccc
      481 ttggtatagg cgtacaggcc tgggatcacc agagctgtta ctaccccata caggatatga
      541 acccaacgcc ctgggcgttc tccaattatc cacaagtaag cgccagtcgc cccttgcaca
      601 agtatcagta gctccgcgat gaccagcgcg ccccagaagc tgctgtccaa tccttgcttg
      661 cgtatggtgc gccacaggcc ccaaattgcc aagatgccaa agtataataa tgcagtgttt
      721 gccaggcgag catgcaatac ttcaacgaat gacatggcgt tctcctcaaa ttaaagaatc
      781 tgaaatttcg aggtcatctt accacattct ctcttcttgc ctcataatac aatgggcaat
      841 ctggatttcc cataatagat tgctagttct tgttccacgg cgcaatcgga ataccaatcc
      901 gaattgcaag aagaacaatc tgatgctgcc aggtcgtggg gtgatgaacg gatcacccgt
      961 ttgtgtagtt caaaatcact ccaattaccc ggcgagacgt gtgacagaag ggaaggtttt
     1021 tggtaaactt agaaagccct aaaacgcgtt taatacacct tatttgtctt gttagatgtt
     1081 ggtattgtga tacaattctc aaaatagtaa ttttgtcaaa tttgtcttat ataaggagag
     1141 taaagctatg accgtaagtc caaatgaggc ggagacagat aaaaaacagg tcacccggcg
     1201 cgagtttttg aatattgctt ggctggcctc cctgggcttc ctgacggtca atctgggtgg
     1261 cgtgacgtat cttttctcga tgcctcgctt taaagagggt gagtttggag gtctctttag
     1321 tgtgggcagg gtttctgagc tcccagaggc aggggcgtct cccgtcaact tccccaaggt
     1381 caagctgtgg cttagcaaca ccgaagaggg tgtcatggca ctctataagg tttgtacaca
     1441 cctgggttgc ttgtacaact ggaactccca agagggcaaa tttatctgcc cgtgccacgg
     1501 ctctcagttt gatgctaatg gtgaatatat tcagggacca gcccctcgtt cgttggaccg
     1561 ttttgtcgtt caggcggttg atcccaacac taaccaggtg ctcgccgagg cggctgatgg
     1621 caagcctatg cctgttcctg accagtctga cgctgtcatt cgagtggata caggcaagcg
     1681 tatcccgggt gatgtccacg cctaaaagca tcccaactca ctttaggttc tagttactga
     1741 gaaggttcta tgctgaaatc gctcaatttc caggatttac gtaaaaaagc cctcgaggct
     1801 ttcgacaatc gggtgcgcat tatcaccgcc ggcatggggt tggatgaact gcgcgctgtg
     1861 gtacgcggtg atccccctac cgagaagcca aatccccgct ataaagttca caccaccagc
     1921 ttcctctttc atattcgccc gcgctattat gaaaagggca gcacgatatt tgctcacacc
     1981 tttcggctag gatttttcac tagcttcttt ttcttcgtcg agctgttcac cggtctgatc
     2041 ctgatgatct attacacccc atctccggct gaggcttaca actcgattct agaattgatg
     2101 agcaatgtcc cctttggaca ggtgctgcgc gatctgcatc gtattggtgc ggaggggatg
     2161 gtcatcttca gcgtgttgca catgctgcgt accttcttga ccggctcata caaaaaagac
     2221 cgcacgttca cctggcttac cggtgtattc ctgctgggga ttaccttatt tctcagcttt
     2281 agcggctatc tgttgccctg ggatcagctc tcctactggg cggtgacgat cggtaccagc
     2341 atggctgagg cggccccatt gttcggcacc caggtcaacc tgctgctgcg cggcgcgccg
     2401 gatattggcg cgggcggttt gttgcgtttt tacctcttcc atgtcgtgct cttcccgctg
     2461 ctggctatct gggtgatcag catccattac tataaagtat cacgcgaaca tggtatctcc
     2521 ctgccggcaa aagttgaaga aggcaatgtc gcgccggaag ctaagaaaga agccaagcag
     2581 cggattgact atatccctga cttgctcacc catgaagtct tcctcactgc tctagggttg
     2641 ttcgtattgg ttgccttggt cacggtcttc aattggcatg ctccactgga acacgtggcg
     2701 aacccgcagc aaacccccct ggacacgaaa gccccatggt acttctggtg gttgcaaggg
     2761 atgcttaaaa ttgacccggc aagcattctc gaaaacctgt tcagcagagt aggcttgcag
     2821 gttgaattga gtgccttatt gaacagcaag cagatcatgg ggatagtctt gcctaccatt
     2881 cttgcagggt tgcttctggg tgtgccttac attgaccgaa acccgcatcg cagtttgtac
     2941 aaaagaccgg tggcggttgg gtttggcctg ctatttaccc tggtgcttgt tgtgctcagc
     3001 tatatgggtc tgcctgagta tggaattcaa acgcccgccg ccacgcgcat catccaggat
     3061 ctggcacctg aagagggtat aggccaattg cgcgaagtgc cgtttgatca attgcgcgtg
     3121 ggtgtgtacg aggtgggtga tactatcccg aggaaccttt gtccggatga acccgcaggc
     3181 tgccccgcgc tgactggcgt atttgccgag ttcagcgagc ggataaattc agcggcgaag
     3241 aatgagcatc ttgagcctta ccagcgactg cccaacgccc aagcggtgat gttgatcgaa
     3301 gattggcagc acgacctgcg caaggtcacc ctgcgtattt tatgggatga cgtcgcttcg
     3361 ggccagccaa agagttatga acgccatgtc ttcctgcacc gtgctcacgg agcggaatag
     3421 catctggcta taggagggtt tctcatcatt atgtcacgag tgcaacttga gatctcattg
     3481 ggcatcctgc tggtgctggc aaccggcacg atcctgattt accagggatt actagaagaa
     3541 gagcgcatgg cgcgcttcga agaacagcag cgcgcccagg cgattgaggt cggggcagag
     3601 ctgtttgata tcaactgcaa gggttgtcat ggcccacaag gcgaaggcac cccaggcctc
     3661 tgcccgccgc tcaacgataa gtatttcttt accgaccgac ttaaagacgt cggctggagc
     3721 ggttccatag aggattacat tgtggcaact gtagccagtg gacggctgac ctccacgcgt
     3781 cccgagctgt accctggtaa tgtgcgccca gccatgcctg cctggtccga gcaatatggc
     3841 ggaccgctgc gtgaggatca gatccgcttt atcgctgctt ttgttgccaa ttgggaagcc
     3901 accgcccccg accggcagtt ggcccctgtt ctggccggac cgcccgtcgg tactgacatc
     3961 acccaggaat tacctgccgg agatgcggtg aaaggcgaag ccctggcctc ctcgcagggc
     4021 tgcattggct gtcacgtggc tgggcctacc ggcccgccct ggaacccaac tgccgatcag
     4081 cccggaattg gcgaccgtgc agccgcacgc ctgacgcaga gtgattactc tggcaatgcg
     4141 gctacgcctg aacaatacct gttggagtcg atcgtgaatc ctgcggctta cctagtgccc
     4201 ggttacgaaa ctgttctgat gcctggtact tacggacaga cgattaccgc tcaagatgcc
     4261 gctgatctga tcgcttacct gcttaccgtg aagtgaggag aatatgccgg tctttcaccg
     4321 catgcgctca tctagccttt ttctctttgt tgctctggtt gctttgctga gctggaattt
     4381 tgtgcacgct cagacatcag gcgaggagca aatggagcag ggcgccagca tctatgctgc
     4441 gaattgcgca gtttgccatg gcgatgatgg tcaagggcgc gtgggtgcta ccctggcgaa
     4501 ggactggcct tccatccgac ccgacttaaa agtgcgcgca acgattgaga atggtattgc
     4561 tggctcgccc atgccggctt ggagccagaa aaatggtggc ccgctcacag acgaggatat
     4621 tgacgccttg gtggtgtaca tcttgagctg ggagacaggt ggcccgcggg tcttcccgcc
     4681 tactcctacc tatatccctc gcccgctcat cagcccggta ccggatgtgg tcggcgatcc
     4741 taatcagggg gcaatcctgt acgaccagaa ttgcgcggtt tgccatggaa ccgaaggacg
     4801 ggggcgcgtt ggcgccagcc tggacaaagt ctggccctca atccgtcccg atctgggcat
     4861 caagaatacc atttcgaatg gggtgatggg ctcgccgatg cccgcctgga gccaggtcaa
     4921 tggcggtccg ctcaccgaga ccgaaatcaa taacctggtg gcgtttatta tggcgttgga
     4981 acctgcaagc gaggaaatcc agcaggctcc tacaccggca cccgcgccag ggccgctggc
     5041 cgattggttc ggtatattgc tggcggtggt cttatttatc ttggtagttt ggatcgcaat
     5101 tgtcctgcaa actcgcactc aggctcaaaa cgatacagag aaatgattga tatgggggcc
     5161 cttgccccca tatcaatcat cattaatcag gttgcttttt aactctcagc cattccagtc
     5221 cgcgaattag taatcctatt tgagc
//
LOCUS       DBNGSX010000261         4533 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_367368, whole genome shotgun sequence.
ACCESSION   DBNGSX010000261 DBNGSX010000000
VERSION     DBNGSX010000261.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_367368"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="AC16CB_07930"
     CDS             complement(<1..379)
                     /locus_tag="AC16CB_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3342435.1"
                     /translation="MEDSNNHQVLLAENLRKSYGPRLALRGLSFSLKAGRILGFLGPN
                     GAGKTTAIRILTTILEPDSGHFVVDGISSEYPDKIRRRIGVLPENLGFPRQMTGLEYL
                     THFGQLYGVSTADARSNSLRLLED"
     gene            complement(444..3578)
                     /locus_tag="AC16CB_07935"
     CDS             complement(444..3578)
                     /locus_tag="AC16CB_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3342436.1"
                     /translation="MMRGIVGSSMHFRYLVIVVAAVIMLFGITQLVNMPVDVLPEFSP
                     PYVEIQTEALGLSTEEVEQMITVPMEQDLLAGVAWLDVIRSESVPGLSSVLVYFEPGT
                     DLYRARQMVAERLAQAAVGLPHVSKPPTMIQPLSSASRFMIVGLSSKELSLIEMSVLA
                     RWTIAPRLMGVPGVAHVAIWGNRDRQLQVLVDPEELQERGVSLQQVVETTGNALWVSS
                     LSYLEASTPGTGGFIDTPQQRLGIWHVLPISSPEELAQVPVKGTAWRLGDVAEVVEDH
                     QPLIGDAVVNDSPNLLLVIQKLPGVNTLEVTQGLDEALDALRPGFAAIDFDATLFRPA
                     TFIEASIANLTRTLVISAFLIVLIIGVFLYGWRTALVSLVAIPLPLVAAVLVLYLRGA
                     TFNAMVLAGLVIALGIIVDDAIVDIEHIMRRLREDHKEGKPKRIEKIILEASIDVRGT
                     IFFATLISLLAVLPVFFMEGVSGALFRSLAVSYALAMIASLAVALIVTPALSVMLLSN
                     APLQDRQSPLIPWLQQSYERALERTTQRPQLAYALVAVLIVAGIVLLPFLGGNQLLPS
                     LREPYLSIRLDAPPGTSHPRMSQIVADASSELRAISGVSNVSAHVGRAVFGDQVVGIN
                     SAELWVSIKPDADYDATVAAVKRQVDGYTGLDHEVSTYMQQALGEPLSSTDDIFTVRV
                     FGEDHDILRDQAQKLQQTVAGINGVVDPHVVLPVEEDSVEIEVDLASAQNYGVKPGDV
                     RRAAATLLSGIQVGSLFEQQKVFDVMVWGAPEVRDSLEDIPELLIDTPRGGHVRLGDV
                     ADVRVVSSPTVIKRIAVSPYLDINFSVQGRDVNAVLGDVEHSLQNFAFPLEYHAELQG
                     DYAARQAIQQRILIAGIVALIGIFLLLQAASESWRLALAILLSLPAALVGGILAAFLS
                     GGSFSLISYFGLFAILGIAARNTVMLIKHYHSLEQEGEEFGLGQVMRGSRERVAPMVM
                     TALTTGLALMPFVLFGNIPGHEIVRPLAIVVIGGLVTSTLLNLFVLPVLYLRFGASRE
                     PDLQFLSVPSASEA"
     gene            4151..>4533
                     /locus_tag="AC16CB_07940"
     CDS             4151..>4533
                     /locus_tag="AC16CB_07940"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3342437.1"
                     /translation="MKVHSLQDEVVGLTRELVRHPSLSGQEQSAAILVRDTMQSLDFD
                     EVWVDELGNVVAARYGDQPGPRLLFDAHLDVVPPTQPEAWTCDPFGGEVRDGRIWGRG
                     ATDIKGGLAALLLGLGNLPRRDLKGA"
BASE COUNT         1163 a         1219 c         1154 g          997 t
ORIGIN      
        1 catcttcgag aaggcgtaag ctattggatc tggcatcagc cgttgatacc ccgtagagct
       61 gaccgaagtg tgtcaggtac tctaagcccg tcatttgcct gggaaatccc aggttctcag
      121 gcaggacgcc gatcctgcgc cggattttgt caggatactc ggaactgatt ccatccacga
      181 caaaatggcc tgaatcgggc tcaagaatgg tggtaaggat gcggatcgcg gtcgttttac
      241 ccgctccgtt tgggcccagg aagcccagga tacgtccagc cttcaatgag aaggatagcc
      301 ctcggagtgc cagcctgggt ccgtaagatt ttcgcaggtt ttctgccagt aaaacttggt
      361 gattgttgct gtcttccatc aattaatctc ctggaatttg ctaaattttg ctcgtctcga
      421 gacccattaa caagtcttca ggtttatgct tcactggcag atggaaccga aaggaattgg
      481 aggtcaggtt cacggctagc cccgaagcgt aaatacagga caggcaggac gaaaagattg
      541 agcaacgtcg aagtgactaa tcctccaata acaacgatag ccagcgggcg tacaatctcg
      601 tgaccaggga tattgccgaa gagcacgaac ggcataagag ccaggcctgt ggttaacgcc
      661 gtcatcacca tcggtgcgac gcgttcacga gaaccgcgca ttacttgccc taatccaaac
      721 tcttcaccct cctgctcaag agaatggtaa tgtttgatta gcatgacggt attacgcgcc
      781 gcaattccca atatcgcaaa cagcccgaag tatgaaatta gcgagaacga gccaccactc
      841 aggaacgctg ccaatattcc gcccaccagc gccgccggca aggagagtaa gatcgccaaa
      901 gccaggcgcc agctttcgga ggcagcctgc aggagtaaga aaatcccgat cagtgccaca
      961 atcccggcga tgagaatgcg ctgttgtatc gcctgccgtg ccgcataatc accttgcagc
     1021 tcggcatggt actccaacgg gaaggcgaag ttttgcagag aatgctcgac atcccccaaa
     1081 acggcgttga cgtcccttcc ctgaacgcta aagttaatat caaggtaagg tgagaccgca
     1141 atacgcttga tgacggtcgg agatgacaca acgcgcacat cggctacatc ccccaatcgc
     1201 acatgacctc cacgaggtgt atcaatcaat aactcaggga tatcttccag gctgtcacgc
     1261 acctcgggcg cgccccatac catcacatca aacactttct gttgctcgaa aagactgccg
     1321 acctgaatac cggagagcaa ggtggccgcc gcacggcgaa catcgcctgg tttgacacca
     1381 taattttgag ccgaggcaag atcgacctcg atctccactg agtcttcctc aacggggagc
     1441 accacatgcg gatcaacgac gccgttgatc ccggccacag tttgctgcag tttctgggcc
     1501 tggtcacgta ggatatcgtg gtcctcgcca aagacgcgca cggtgaagat atcatctgtg
     1561 ctcgacagtg gctcacccag cgcttgctgc atataggtgc tcacctcatg atcaagtccg
     1621 gtgtacccat ccacctggcg cttaacagcg gctaccgtcg catcatagtc ggcgtcaggt
     1681 ttgatgctga cccacaactc agcggagtta attccgacca cctgatctcc gaacactgcc
     1741 cgtccaacgt gagcactgac attactgacc ccggaaatgg cgcgcagctc actgctcgca
     1801 tcagccacaa tctggctcat tctcggatga gatgtgcctg gtggagcatc cagacgaatt
     1861 gaaaggtatg gctcccggag cgaaggaagc agttgattcc cccctaggaa tggcagcaac
     1921 acaataccag caacaatcag aacggcgaca agcgcatacg ccagctgggg tctctgggtg
     1981 gtacgctcca gagcacgttc gtagctttgc tgcaaccacg ggatgagggg tgattggcga
     2041 tcttgaagcg gcgcattaga aagcagcatt acgctcagcg ctggcgtaac gatcagtgcc
     2101 acagctaaag aggcgatcat cgccaaagcg taggagacag ctaatgacct gaacaatgcg
     2161 ccggatactc cttccataaa gaagaccggt agcacagcca gcaggctgat cagagtggca
     2221 aagaagatgg taccgcgcac atcgatcgag gcttccagga taatcttctc gattctcttg
     2281 ggtttgccct ccttatggtc ttcgcgtagg cgccgcatga tgtgttcgat atcgacgatg
     2341 gcatcgtcga caatgatgcc taaggcgatt accaagcccg ccaaaaccat cgcattaaac
     2401 gtagcccccc gcaggtacag gacgagcaca gcggctacca gtggcaatgg gatcgccacc
     2461 aggctgacaa gcgctgttcg ccaaccgtat aggaatacgc cgattatcaa aacgatgagg
     2521 aacgcagaga tgaccaacgt ccgggtgaga ttagcgatcg acgcctcaat gaaggtcgct
     2581 ggacggaaga gggtagcgtc aaaatcaatc gcggcaaacc cgggcctcaa cgcatctaac
     2641 gcttcatcca acccttgtgt cacctccagg gtgttgaccc caggaagttt ttggataact
     2701 agcagtagat tagggctgtc attgacaact gcatccccga tcaacggctg gtgatcttct
     2761 actacttcgg ccacgtcacc taggcgccaa gcggtgcctt tgactggcac ttgagccaat
     2821 tcctctggcg aggagatggg caagacgtgc cagatcccga gcctttgttg gggggtatca
     2881 ataaagcctc cagtcccagg cgtcgacgcc tctaagtagc tcaacgagga tacccaaagc
     2941 gcatttccgg tcgtctctac cacttgctgg agcgatacac ccctttcctg taactcttcc
     3001 ggatcgacca gcacctgcag ttgtctatcc cggttgcccc aaatagcgac atgggcaact
     3061 ccgggcacac ccatcaagcg cggagcgatt gtccaacggg caagcaccga catctcgatc
     3121 aaggatagtt cctttgagga aagccccaca atcatgaacc ggcttgccga ggacaagggc
     3181 tgaatcatgg ttggaggttt tgagacgtgg ggcaggccga cggctgcctg ggcaagacgc
     3241 tctgccacca tctgccgtgc acggtagaga tcggtcccgg gttcgaagta gacaagcacc
     3301 gatgaaagtc ctggcacaga ctccgagcgg atgacatcca gccaggccac acctgcgagc
     3361 aaatcctgct ccatcggcac ggtgatcatc tgttcaactt cctccgtgga aagacccagg
     3421 gcttcggtct gaatctctac ataaggtggc gaaaactcgg gcaacacatc cacgggcata
     3481 ttaaccagtt gggtgatacc aaagagcatt atcacagctg cgacgacaat caccaaatat
     3541 cgaaagtgca tgcttgatcc aacaatccca cgcatcatgg ctgattacct ccttgctcac
     3601 agttgacttt tgcaccagaa aagcgtatct attaagttat gaatgaacaa tttcttagtg
     3661 agattgcgtc gaagtaagaa atacatcgag cattaaatga aaaaatacat aacctcggct
     3721 atggtccaat cgaactgtcg tgctgtatgc tagttatcaa gatcatcatt tgccactcga
     3781 gtaatccgct ctagacaacc gcatggcaat actatttccc actattaata ggcgaaaccc
     3841 accttaagtt atcaatgaaa ccgctaacta ccaactttga tatatgagcc cctatacgat
     3901 tattatgtgt ttataatctc tctaagaatg cgattaattg acagctaatg tgtttttaac
     3961 ctggacttaa ttttatcaca caaaaatctt aatgtcaata tatatgacaa aattagtctt
     4021 gttttgatat attattaggt tgtttcccct gttggttgtg catcgggaca acgacctaaa
     4081 ggcgatgaga tatcaatatt tggtaggatt atcacattgg cctgttggtc ggatgattgg
     4141 aggtgctgga ttgaaagtcc actctttaca agatgaagta gtaggtctta cccgcgagct
     4201 ggtacgccat cccagtcttt caggtcagga gcaaagcgct gctatactgg tgcgcgatac
     4261 catgcaatcc ctagatttcg acgaagtttg ggtagacgag ttgggcaacg tagtggcggc
     4321 gcgctacgga gatcagccag gtccacgcct actgttcgac gcccatctgg atgtggttcc
     4381 ccctacccag cctgaagcct ggacatgcga tccatttggg ggtgaggtgc gggatggacg
     4441 tatctggggg cgcggtgcga cagacatcaa aggaggctta gcagctcttc tgttgggctt
     4501 aggtaatttg ccgcgtcgtg acttaaaagg cgc
//
LOCUS       DBNGSX010000262        11113 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1821752, whole genome shotgun sequence.
ACCESSION   DBNGSX010000262 DBNGSX010000000
VERSION     DBNGSX010000262.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11113)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11113)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11113
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1821752"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1227)
                     /locus_tag="AC16CB_07945"
     CDS             complement(7..1227)
                     /locus_tag="AC16CB_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015208407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR3342438.1"
                     /translation="MSTSMTTEMREAMRPPEPPGPIAWLQKNLFNNWYNSLLTVLALG
                     FIVWASVGIYRWVFFTADWEPVSANLMLYLVGQYPREALWRLGAIITIMAFLFGMSWR
                     RWGGVMRTFAVTFIVFIVSVALWPAEVSGFTIVSRGVLLGNILVLLAGYLVAGWKRMR
                     NTYLLLGWLISLPITLLLLSGVTEISWLPPIATGMWGGMIVTLLLAIGGITISFPIGV
                     FLALGRRSSLPVVSLLCTAFIEVIRGVPLIGLLFLSSLILPLFLPPDVRIDRLLRALI
                     GMTLFSAAYTAENVRGGLAAVPNGQVEAARSLGMGNLYITTMIVLPQALRAVIPPIVG
                     QFISLFKDTTLASGVAVLELLSIGRSILQSDPAFIGLQMEVYLFVALVFWIFSYLMSY
                     ASQRLEVALGVGER"
     gene            complement(1224..2423)
                     /locus_tag="AC16CB_07950"
     CDS             complement(1224..2423)
                     /locus_tag="AC16CB_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR3342439.1"
                     /translation="MTQKPTTTQRFQLKNIATLWWDERVLRLMAQLISAILVVGFLVL
                     AVQNVISAAEQRGLSLGFQFLKDAAGFPIGESDIPYDPSQSFAYAFWVGVVNTIKASL
                     VGVILATILGTIVGITRLSSNWLVNRISLAYIEFHRNIPLLVLLLLWYRGVFTKLPRV
                     QDSITFPGPLYLNQRGLYMTWPKLTSQGTPFVVAALIGIVLAVIAWIWLRRVREVSGR
                     STYFGAVSLGILILSPLIGWWLSGGQPFIFDEPVLKGFNFKGGYYLTPEFIALLVGLV
                     MYTAAFIAEVVRAGIQAVSRGQLEAARALGLRYPQVLGLVIMPQALRIIIPPLISQYL
                     NLTKNSSLAVFIGYPELFFIGKTTINQAGRAVQVFVLIMGVYLTISLFTSVVLNIYNR
                     RIQLVER"
     gene            complement(2488..3663)
                     /locus_tag="AC16CB_07955"
     CDS             complement(2488..3663)
                     /locus_tag="AC16CB_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3342440.1"
                     /translation="MTRKTLPLFSILIAIALIAAACGGGAPAAPAGEAPAPVVETVEV
                     VKTVVVTVEVPVEAGEQPVATPEVQIIQPQGYGTIVQRIKDRGSVVCGGRTDLAGFGT
                     LDANGRNEGFDIDLCRAIAAALLGDPEAVEVVPLSAAERGPALQTGEVDILSRNATWT
                     SSRDAQWGNFTSIWFYDGQGFMVPADSGITTIEELDGATVCVTSGTTTELNLADAFRQ
                     RNLEFTPVVFEDTATVYGTYEEGRCDATTSDKSQLASVRSGFQDPSAHVILDITISKE
                     PLTPAVPTGDDQWFDIVKIVIYGLVNAEELGVTQANVDEMVNSDNIEVRRLLGSEGEW
                     GQADLGLPADAIVQAVKAVGNYGEIYDRYIGPNGLNIPRGPNNLWTNGGLIYAPPMR"
     gene            complement(3961..4431)
                     /locus_tag="AC16CB_07960"
     CDS             complement(3961..4431)
                     /locus_tag="AC16CB_07960"
                     /inference="COORDINATES: protein motif:HMM:NF015999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HIR3342441.1"
                     /translation="MITNNVTRLLDQRKINYNAFELPPEKLGAIEAAQFLGVSPGLVY
                     KTIVITREGKGKPILAVVPGDREVDRKALASAAGEKKVFVPTQREAEQLTKLQAGGIS
                     PLALINRGFQVIIDASAQVLPEIFISGGQRGLNIRLAPSDLASLTNARFAPICF"
     gene            complement(4476..5873)
                     /locus_tag="AC16CB_07965"
     CDS             complement(4476..5873)
                     /locus_tag="AC16CB_07965"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3342442.1"
                     /translation="MRLRLFLSFAIIVLVSIASVVVIARQGAATEVRAFMFRGGMSGT
                     ESLVSSLEAYYAAHDSWQGVEQLLLESGPGRGHGMPGMGGNGLGPGGMAGMMAQRLRL
                     ADAQGVLVFDTSQNGVGGTLSSDELQSAISLRAGGKVVGFLLPEGGMAFSPGDETRLV
                     SRITRAALVAGMVAGIASLLLALVLAYRLLRPIHALTRAAEQMGGGDLSQRVPVHGDD
                     ELALLGTTFNRMAASLQRAEESRQAMTADIAHELRTPLAVQRASLEALQDGIYPLDSE
                     NLAPILEQNILLNRLVDDLRTLALADAGQLQLEIIPTDFDGLVEHVVELFTPQAASQS
                     IDLHIDNSPAHLSPIPLDPLRVEQILGNLLSNALRHAPPHSQVDIAISHTPTAVQLSV
                     RDSGPGISPEALPRVFERFYRADKSRSRVEGGTGLGLAIARQIAQAHGGNLTADNHPL
                     GGAIFTLTLPSAQNP"
     gene            complement(5927..6631)
                     /locus_tag="AC16CB_07970"
     CDS             complement(5927..6631)
                     /locus_tag="AC16CB_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3342443.1"
                     /translation="MATILVIEDEPELVKVLRSYLEQAGFSVLTAARGDTGLSTWEHK
                     RPDLVLLDLNLPGMDGLDVAREIRRKSETPIIMLTARVEEIDQLIGLELGADDYIPKP
                     FSPRLVVARVRALLRRAASTPTASQVLRIADLEIDLDAYTVARSEQLLELTPTEFNLL
                     AAMAAQPGRVFTRLQLLEAVQGVAYEGYERTVDAHIKNLRAKLEPDPKQPRYIETVFG
                     IGYRFRKETRDLPSEA"
     gene            complement(6721..7242)
                     /locus_tag="AC16CB_07975"
     CDS             complement(6721..7242)
                     /locus_tag="AC16CB_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342444.1"
                     /translation="MKKFLVISAVAILAILVLGVSGFAYAQAQTPPPPDYPNYGPGMM
                     GRWGGRGFGQGMMGGWGQGGYGPMHEYMEDAFAEALNLTHEELEAKLADGETMWSIAQ
                     AQGLSDEEIADLMVNARTKALEQAVADGVLTKEQADWMTQRMQEMQSAGFGPGYCHGF
                     SGQGRGSGWRNTP"
     gene            complement(7415..9451)
                     /gene="uvrB"
                     /locus_tag="AC16CB_07980"
     CDS             complement(7415..9451)
                     /gene="uvrB"
                     /locus_tag="AC16CB_07980"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIR3342445.1"
                     /translation="MDFKLNAPFKPTGDQPEAIKKLTNGVQKGHRHQVLLGATGTGKT
                     YTIASVIEKLNMPALVMAHNKTLAAQLYAEFKEFFPENAVEYFVSYYDYYQPEAYVPR
                     HDLYIEKDSDINEEIERLRLAATTALMSRKDVIIVASVSCIYGIGNPDAYGRVVINLE
                     SGTVYRRNALLRQLIEGQYERNDLELSPGKFRVRGDTLEIIPAYQDQFGYRITFFGDE
                     VEKVVEFDAITGELRRQLSTVHIYPAKHFITDQDKLNQAIEDIQAEMVERLALFKSQD
                     KHLEAQRLEQRTYYDLEMLREVGYCSGIENYSRHLDQRPQGSPPWTLMDYLPSEYLLV
                     IDESHMTIPQVRGMFNGDRSRKETLVEYGFRLPSALDNRPLMFDEFERRMGFTIYTSA
                     TPGPYEMKRNDQVVEQIIRPTGLVDPEVEVRPIEGQIDDLLAEIRQRVEVGQRTLVTT
                     LTKRMAEKLADYLMELGVKVHYLHSEIDTLERVGILRDLRLGVFDVVVGINLLREGLD
                     LPEVSLVAILDADKEGFLRSETALIQTIGRAARHVDGRVIMYADKITDSMRRAIDETN
                     RRRAKQVAYNQEHGIQPVSIYKAVRDLTDQLSVRAVAESKPEYRVRGGSMERSEMQRV
                     INELEKQMKEAAQNLEFEKAAVLRDQVFELRGLLAEDSNLPPWKKVRLLAGEED"
     gene            complement(9505..10014)
                     /gene="tnpA"
                     /locus_tag="AC16CB_07985"
     CDS             complement(9505..10014)
                     /gene="tnpA"
                     /locus_tag="AC16CB_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015205995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HIR3342446.1"
                     /translation="MSFWRLYYHLVWATKNREPSITPEVELKLYPYLLKKAAELEIGV
                     YALNGWYDHVHMIVSIPPKLAVADAVKYLKGASAHYLNHEGTLGGAFIWQRGYGALSV
                     GERQKSIAIAYVESQKEHHKQGTTNVWLERCTDLDEKSSDSGIMTGTISSGIREERET
                     YEPIGDSLI"
     gene            complement(10272..>11113)
                     /locus_tag="AC16CB_07990"
     CDS             complement(10272..>11113)
                     /locus_tag="AC16CB_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase
                     domain-containing protein"
                     /protein_id="HIR3342447.1"
                     /translation="KDHLLVFGANRELATLAHNPQLLIDGVQNANGISFIAHPVDPAA
                     PAVGEPDISWESWNVQGYTGIELWNAFSEFKSLLRSRLHAAYYAFNPNRIARGPHPDA
                     VRKWDELLNAGRRVVAIGGSDAHALPKSMGPLRRTIFPYEFHFRTVNTHLFLASPLSG
                     EVAEDRQLVLDALKGGHAFIGYDLPAPTRGFRFTAQGKDILAWMGDSLPSKNGVTLQI
                     RLPRRTECRLLKDGKTIKSWNNRETCTHITSEPGVYRVEVDIDYLGRKRAWIISNPIY
                     VKE"
BASE COUNT         2531 a         2990 c         3052 g         2540 t
ORIGIN      
        1 tttattttac ctctcaccaa cgccaagcgc aacttccagt cgctggctgg catacgacat
       61 gaggtaactg aaaatccaaa atactaacgc gacaaataga taaacctcca tttgcagtcc
      121 gataaacgca ggatcagatt gcaatatgga acgcccaata ctaagtagct ccaacactgc
      181 aacgccggag gccagggttg tgtctttgaa caagctgatg aactgcccaa cgatgggggg
      241 gattacagcc cgcagtgcct ggggaagcac aatcatggtg gtgatataca aattgcccat
      301 tcccagcgag cgagcagctt caacctgtcc atttggtaca gctgccagcc cgccgcgcac
      361 gttttcagcg gtgtaggccg cgctgaataa ggtcataccg atcagggcgc gtaataaccg
      421 gtcaatacgc acatctggtg gtaaaaagag aggcaggatc agagaggaca agaatagcaa
      481 gccaattaat gggacaccgc gtatcacctc aataaatgcc gtgcaaagca ggctaaccac
      541 cggcaacgac gagcgtcggc ctagcgccag gaatacgccg atagggaatg agatcgtgat
      601 tccgccaatg gcaagcaata aggtcacaat cattccaccc cacatacccg tagcaattgg
      661 gggcagccaa gatatttccg ttactccgct cagcaggagc agggtgatgg gcagtgatat
      721 caaccagcca agtaagaggt aggtattgcg catccgcttc cagccagcca ctaggtaacc
      781 tgcgagaagt acaagaatgt ttcctaatag gacaccgcga gatacaatcg tgaaaccgct
      841 aacttcagct ggccagagag caactgaaac aataaagacg atgaatgtga ccgcgaaggt
      901 ccgcatcaca ccaccccacc gccgccagct catgccaaat aagaatgcca ttatggttat
      961 tatggcgcct aatcgccaca aagcctcacg cgggtactgg cctaccaggt agagcatcag
     1021 gtttgctgat accggttccc aatctgcggt gaagaaaacc caccggtaga tgccaacgct
     1081 tgcccagacg ataaagccca atgcgagtac cgttagcagg ctgttgtacc aattgttgaa
     1141 caggttcttc tgtaaccaag ctatcgggcc gggtggctct gggggtcgca tagcctcacg
     1201 catctctgtt gtcatgcttg tgctcatctt tccaccagtt ggatacgacg attatagata
     1261 tttaggacta cggaggtgaa caggcttatc gtcaggtata ctcccatgat taatacaaag
     1321 acttgcacgg cacgtccggc ctgattaatg gttgttttgc caatgaaaaa taattccgga
     1381 tatccaataa atacggccag gctggagttc ttggtcaggt tcaggtattg gctgatgagc
     1441 ggcgggataa tgatgcgcaa tgcctgaggc atgatcacca gcccaagcac ttgagggtag
     1501 cgcaatccga gggcgcgtgc ggcctctagc tggccgcggc tgacagcctg gatgccggcg
     1561 cgcactacct cggcaataaa agcggctgtg tacatcacca aaccgacaag cagagcgatg
     1621 aactccggcg tcaggtagta gccaccttta aagttgaatc ccttgagtac cggctcgtcg
     1681 aagatgaagg gttgaccacc ggagagccac cagccaatta aaggtgacaa tatcaatatg
     1741 cccaggctga ccgctccaaa ataggtggat cgcccgctga cctctcttac tcggcgcaac
     1801 caaatccagg cgattacagc caggactatc ccgatcaaag ctgcaactac aaatggtgta
     1861 ccttgggagg tgagtttggg ccaggtcatg tacaggccgc gctggttcaa gtaaagcggg
     1921 ccgggaaagg tgatgctatc ctgtacgcgg ggcagcttgg tgaaaacccc tcggtaccac
     1981 aacaacaata agaccagcaa tgggatattg cgatggaatt caatataagc taatgagatc
     2041 cggttgacaa gccagttact ggataagcgg gtaatgccga caatcgtgcc aagaatggtg
     2101 gccaggatga cccctaccag ggatgccttg atggtgttca ccacaccgac ccagaaggca
     2161 tatgcgaagg attgcgatgg gtcgtaaggg atgtcagatt ccccaatcgg gaagccggcg
     2221 gcatccttca gaaattggaa acctaacgac agcccgcgct gctcggcagc gctgatgacg
     2281 ttttgcactg ccaaaaccag gaaaccaacc accaggatgg cggaaatcaa ctgcgccatg
     2341 aggcgcagca cgcgctcatc ccaccatagc gtggcgatat ttttcagttg gaacctttgg
     2401 gttgttgttg gtttctgtgt cattggatta atcaagagag gaggcacccg gtcaggccgg
     2461 gtgcctctcc catattaagt tttcgactta gcgcataggc ggcgcgtaga tcaggccgcc
     2521 attagtccac aaattgttcg ggccgcgcgg gatattcaat ccattcgggc cgatataacg
     2581 atcatagatc tcaccatagt tgccgactgc cttgactgct tggacaatgg catctgccgg
     2641 taaaccgagg tcagcctgac cccattcacc ttcgctaccc aataggcgcc gtacttcgat
     2701 gttgtcgctg ttcaccatct catcgacgtt ggcttgggtg acgcccaatt cttcggcgtt
     2761 aactaagccg tagatgacga ttttaacaat atcgaaccac tggtcgtcgc ctgtaggcac
     2821 cgccggtgta agcggctctt tggagatggt gatgtccaga attacgtgag ccgacgggtc
     2881 ttggaagccg ctccgtacgg atgctagctg tgatttgtcg ctggtggtcg catcacaacg
     2941 tccctcttcg taggttccgt agacggtcgc agtgtcttca aagacaaccg gggtgaattc
     3001 caggttgcgc tggcggaagg cgtctgcgag gttgagctcg gtggtcgtgc cgctggtgac
     3061 acacacggtt gcgccatcca gctcctcgat ggtggtgatg ccactatcag ccgggaccat
     3121 aaagccttga ccatcgtaga accagatcga ggtgaagttg ccccactggg catcgcggct
     3181 gcttgtccag gtggcgtttc gtgagagaat atccacctca ccggtttgca gggcagggcc
     3241 tcgttctgcg gcgctcagag gcactacctc aactgcttcg ggatcgccca gtaatgcggc
     3301 tgcgattgcc cggcacaggt cgatatcaaa gccctcattg cgaccgttcg cgtccaaagt
     3361 gccgaaacca gccaggtcgg tgcgcccgcc gcagacaacg ctgccgcggt ccttgatgcg
     3421 ttgcacaatg gttccataac cttggggctg gataatctga acttcgggtg ttgctacggg
     3481 ttgctcgcca gcctcaactg gcacttcgac cgtaacaacc acggtcttga caacctctac
     3541 agtctcaact acaggggctg gcgcttcacc tgctggtgcg gcgggtgcac ctccaccgca
     3601 ggcagcggct attaatgcta tagcgatcag gatagaaaac aggggtaaag ttttacgtgt
     3661 catcgagttc ctctcctccg aaggatgaat ttgactaact acctaacaaa ctactactaa
     3721 aattgtcgaa tatctacttg ttaagggtac tttctgggat caccacctcc tctcacaatt
     3781 tgggatgcaa ctcgataata agcccatcgg ttgcattatc gatagccagt atatgctcat
     3841 ttcgcattgg cgttattgca ggaacgttga gcagacgagc tttgggctgc gaaaatctaa
     3901 ctgggtaatt atagggaaaa aactcagtaa aggcaaggta taaactgcaa atcttttgca
     3961 ttaaaagcag attggggcga aacgggcgtt tgtcagagac gccagatcag agggagccaa
     4021 gcgaatattc agaccacgct gaccaccgga gataaatatt tcaggcagca cctgagcaga
     4081 ggcgtcaata attacctgaa agccacggtt aattaaagcc aacggggata tgccccctgc
     4141 ttgtagtttg gtaagctgct cggcttcccg ctgggtgggt acgaatacct ttttctcgcc
     4201 cgctgcactc gccagggcct ttcgatctac ctcccggtca cccggcacca ccgctaggat
     4261 aggcttgcct tttccctcac gggtaatgac aatggttttg tagaccaacc ccggagatac
     4321 tcccaggaat tgggctgctt ctatggcgcc cagtttttca ggtggcagct cgaaggcgtt
     4381 gtaatttatc tttcgctgat cgagcagtcg ggtgacattg ttggtgatca tcttttcaat
     4441 gaaatgattg agaaatattg taatacgaac acgctttatg ggttttgtgc cgatggcagg
     4501 gttaatgtga agatcgcccc tcccaaggga tggttgtcgg cagtcaaatt gcctccatgc
     4561 gcttgcgcaa tctgccgggc aattgccagg cccaagccgg tgccgccctc cacgcggctt
     4621 cgggatttgt cggcgcggta aaagcgttcg aacacgcgcg gcaaggcttc tggggagata
     4681 cctgggccgc tgtcgcgcac actcagctga actgcggtag gcgtatgtga gatagcgata
     4741 tctacctgac tatgtggggg cgcgtggcgc agggcattag atagcaggtt gcccaggatt
     4801 tgctcgaccc gcagtggatc cagcgggatg ggagataaat gcgcaggaga attatcaata
     4861 tgcaggtcaa ttgattggga agcagcctga ggggtgaaaa gttcaacgac gtgctcgacc
     4921 agcccatcaa aatctgtggg aataatttct aactgtaact ggccggcatc ggccaaggcc
     4981 agggttcgca ggtcgtccac cagtcgattg agcagaatat tctgttccag tatgggcgcc
     5041 aggttctccg aatcgagcgg gtaaatgccg tcttgaaggg cttccaggct ggcgcgttga
     5101 acagctaggg gagtgcgcag ctcatgggcg atatcggcgg tcatcgcctg gcggctctcc
     5161 tcagcgcgct gcaaagatgc ggccatgcgg ttaaacgtcg tgcctagcaa ggccagctcg
     5221 tcgtctccgt ggactggcac tcgctgagag aggtcgcccc cgcccatctg ttcggcggcg
     5281 cgagtcagtg cgtggatggg tcggagcaaa cgataggcga gaaccagcgc caagagcagc
     5341 gacgctatac ctgcgaccat cccagcaacc agggcagcgc gtgtgatgcg gctgaccagg
     5401 cgggtctcgt cgccgggcga gaaggccatg cccccttcgg ggagcaggaa accgacaact
     5461 tttccgcctg cccgcaggga gattgcactt tgcagttcgt ctgagctgag cgtacctccc
     5521 acaccgttct ggctggtatc gaaaactagc acgccctgcg catcggcgag ccgcaggcgc
     5581 tgggccatca tccccgccat gccacccggc cccagaccgt tgcctcccat gccaggcatg
     5641 ccgtgcccgc gccctggccc cgattcgaga agaagctgct cgaccccttg ccaggaatcg
     5701 tgggcggcgt agtacgcttc cagtgagcta accaaactct ctgtccctga catgccgcca
     5761 cgaaacataa acgcgcgcac ctcggtggcg gcgccttgcc gggcaatgac tactacgctg
     5821 gcaatggaga ccagcacgat gatggcaaag gaaaggaata aacgtaaacg catagcttgg
     5881 cgatgagagg tcttggttgt ggtatcaggg tgtcaagctt atttatttag gcttcggacg
     5941 gcaagtccct ggtctcttta cgaaaccggt atccaatacc gaatacagtc tcgatgtaac
     6001 ggggttgttt tgggtcgggt tccagcttgg cgcgcaggtt cttgatatga gcatcgacgg
     6061 tacgttcgta gccttcgtaa gctacaccct ggactgcttc aagcagttga aggcgggtaa
     6121 aaacacgccc gggttgagcc gccatcgctg ccaggaggtt aaattcggtg ggtgtcaact
     6181 ccagcagttg ttcagaacgg gcgacggtat aggcgtcaag atcgatctcc agatcagcaa
     6241 tgcgcaatac ctgagaagct gttggagtgg aagcggctcg gcgaagtaga gcgcgcacgc
     6301 gcgccactac aaggcgcggc gagaagggct tggggatgta gtcatcagca cctagctcca
     6361 ggccaattaa ctggtcgatc tcttcgacgc gggcggtaag cataatgatg ggcgtttcgc
     6421 tcttgcggcg gatctcacgg gctacatcca atccatccat gccgggcaga ttcagatcga
     6481 gcaacactag gtctgggcgc ttgtgctccc aagtggacag ccccgtatcg ccgcgcgccg
     6541 cggttaaaac actgaaaccg gcttgctcca agtaggagcg cagcaccttg accaactcgg
     6601 gttcgtcttc aataaccaaa atcgttgcca ttaccgcgca cattatacaa caagcaaggg
     6661 gaagccgaaa aaaaagggtt gtgggtgctg aaacacccgc aacccttctc tacggatatg
     6721 ttatggtgtg tttcgccagc ccgaaccgcg accctgaccg gagaaaccgt gacagtagcc
     6781 tgggccaaaa ccggccgatt gcatctcctg catgcgctgg gtcatccagt cagcctgctc
     6841 cttggtgagt acaccgtcag caacggcctg ttccagggct tttgtgcggg catttaccat
     6901 caggtcggca atctcctcat cggataagcc ttgagcctgt gcgatgctcc acatggtttc
     6961 accatctgct agcttggcct ctagctcttc gtgagtcaga ttcaatgcct ctgcgaaggc
     7021 gtcttccatg tattcgtgca tcggaccata gccgccctga ccccagccac ccatcatacc
     7081 ctggccaaac ccgcgaccac cccagcggcc catcatgccg gggccataat tagggtaatc
     7141 aggcggagga ggggtctgtg cttgagcata ggcaaagcct gaaactccca aaaccagaat
     7201 ggccagaatg gcaactgcgc taattactag gaactttttc atctcaaata ctccttaatg
     7261 aagtgatatg tgttggttgt tttgtttacc aatctgtcct tagtttagcg cagggttgtg
     7321 aagaagttgt gaagagcttc tgtacacttc gtgaaggttt gcacagtaat caagatgttt
     7381 ttccccgcta aaacaattgt atattaggcg gtggtcaatc ttcttcgcct gccagcaggc
     7441 gaactttctt ccagggcggc aggtttgaat cctcggctag aaggccgcgc agctcaaata
     7501 cctggtcgcg cagcacggcg gccttctcaa actccaggtt ttgagccgct tctttcattt
     7561 gcttctccaa ctcattgatg acacgctgca tctccgagcg ttccatgctc ccaccccgta
     7621 cgcggtattc gggcttgctc tccgcgacgg cacgcacgga tagctggtcg gtcaggtctc
     7681 tcacggcctt atagatgcta actggctgga tgccatgctc ctggttatag gctacctgct
     7741 tggcgcggcg gcgatttgtc tcatcgatgg cgcggcgcat tgagtcggtg atcttatctg
     7801 catacatgat caccctccca tctacgtggc gggcggcgcg accaatggtt tggatcaggg
     7861 cagtctcgga gcgcaggaag ccctctttgt ctgcgtctag gattgccacc agagagacct
     7921 cgggcaggtc aagaccctcg cgtagcaggt ttatcccgac caccacgtca aataccccca
     7981 gacgcaaatc acgcaggata cccacccgct ccagggtatc aatttcggaa tgcagataat
     8041 gaaccttgac acccagctcc atcaggtaat ctgcaagttt ctctgccatg cgcttggtca
     8101 gggtggtcac cagagtgcgt tgaccaactt ccacccgctg gcggatttcg gctagcaggt
     8161 cgtcgatttg gccttcgatg gggcgcactt ccacttcggg gtcgaccaac ccggtggggc
     8221 gtatgatctg ttccacgact tggtcgttgc gtttcatctc gtatgggccg ggcgtagcag
     8281 atgtataaat ggtgaagccc atgcgccgct cgaattcgtc aaacataagt gggcggttat
     8341 ccagcgcaga cggcaatcgg aagccatatt ccaccaatgt ctctttgcgc gagcgatcgc
     8401 cgttaaacat gccgcgcact tgggggatgg tcatgtgtga ttcgtcgata accagcaggt
     8461 actcgctggg caggtagtcc atcagcgtcc agggaggtga gccttgcggg cgctgatcta
     8521 ggtggcggga gtagttctcg attcccgagc agtaacccac ctcgcgcagc atctctagat
     8581 cgtagtacgt gcgttgctcc aagcgctgtg cctccaggtg tttgtcctgc gacttaaaca
     8641 aggcaaggcg ctccaccatc tcagcctgaa tatcttcaat agcctgattg agcttatcct
     8701 ggtcagtgat aaagtgcttg gctggataaa tgtggacggt gcttagctgg cggcgcaact
     8761 cgcccgtgat ggcgtcaaat tccaccacct tctctacctc gtcgccaaaa aatgtgatgc
     8821 gataaccgaa ttggtcctgg tacgccggga tgatttccag tgtatccccg cgcacgcgga
     8881 atttacctgg gcttagctct aggtcgttgc gttcgtactg tccttcgatg agttggcgaa
     8941 gcagggcgtt gcggcgatag acggtgccgc tctctaggtt gattaccacc ctcccatatg
     9001 cgtctggatt gccgatgcca tagatgcacg agacggaggc gacgatgatc acgtccttgc
     9061 gtgacatcag ggcggtagtt gcggcgaggc gcaggcgctc aatctcctcg ttgatgtcac
     9121 tatctttctc gatgtacaag tcgtggcgcg ggacgtaggc ctcgggttgg tagtagtcgt
     9181 aatacgagac gaagtactca acggcgtttt ctggaaagaa ttccttgaat tcggcgtaca
     9241 gttgcgccgc cagggtttta ttgtgcgcca tcaccagggc gggcatattg agcttttcga
     9301 tcaccgaggc gatggtgtaa gtcttccccg taccggtcgc acccaaaagc acctggtggc
     9361 gatggccttt ttgaacccca ttggttaatt ttttgatggc ttccggctga tcgccggtgg
     9421 gcttaaatgg ggcgttgagc ttaaagtcca taataatgga ttataccaga acaaaagttc
     9481 agtttagtgc gtttcagtgt tcggctaaat tagagaatca cctattggct cgtatgtctc
     9541 ccgttcctcc ctaatgcctg aagaaatagt gccagtcatt attccggaat cagatgattt
     9601 ctcatcgaga tctgtgcagc gctccaacca gacattggtt gtaccttgtt tatggtgttc
     9661 cttttgcgac tcgacgtaag caattgctat tgacttttga cgttcgccaa cacttaacgc
     9721 gccataaccg cgttgccaga taaaagcccc gccaagcgtc ccctcgtggt tcagatagtg
     9781 cgcgcttgcc cccttcagat acttgacagc atccgccaca gcgagtttgg gtggaatcga
     9841 aacaatcata tggacgtgat cataccagcc attcaaagcg tacacgccga tttctagctc
     9901 ggcggccttc ttcagcaaat atgggtagag ctttagctca acctctggag tgattgaagg
     9961 ctcacggttc tttgtagccc agacgaggtg ataatacaag cgccagaatg acatagatca
    10021 ctccttggtt ttagtaaacc cgctttagcg ggtttcggct agctagccgt cgaatttatt
    10081 cgatggcgat gatttcttca tcgtccttct ttgcttcttc gcatccgcca gcgttgaaac
    10141 gcccggctgg ggatattcaa gcccgttgaa aacgggctgt aacgcggtgg tccgatttca
    10201 acccgcttga gcgggttttc gctatggtag ccgtcgaatt tattcgatgg cttaatgcat
    10261 tcgatggcgt cttattcctt cacatagata gggttgctga taatccaggc tctttttctg
    10321 cccaggtaat caatatccac ctccacccgg tacacaccgg gctcggatgt gatgtgtgta
    10381 caagtctccc ggttgttcca ggatttgatg gttttcccat ccttaaggag gcgacattcc
    10441 gtgcggcgcg gcaagcgtat ctgcagggtg accccgtttt tagacggcag gctgtcgccc
    10501 atccaggcca aaatgtcttt gccctgggca gtgaatcgaa acccccgcgt cggtgcgggt
    10561 aaatcgtagc caataaaagc gtggccgccc ttcagggcat ctaataccaa ttgccggtcc
    10621 tcggcgactt cgccgctcag aggggaggct aagaaaagat gggtattgac ggtgcggaag
    10681 tggaactcgt atgggaatat cgtgcgccgt agcggtccca tgcttttggg caaggcgtgg
    10741 gcatccgagc ctccgatggc gaccacccgc cgccctgcat tcaacagctc atcccatttg
    10801 cgtactgcgt cggggtgcgg cccacgcgcg atgcggttag ggttgaaggc atagtaggca
    10861 gcatgcaggc gcgatctgag tagcgactta aattctgaga aagcattcca aagttcgata
    10921 cctgtgtagc cctgcacgtt ccagctttcc catgagatat ctggctcgcc caccgctggt
    10981 gccgctgggt caaccggatg ggcgatgaat gaaataccgt ttgcattctg tacaccatcg
    11041 ataaggagct gcgggttgtg cgccagtgta gccagctccc ggttggctcc gaaaaccagc
    11101 aaatgatcct ttt
//
LOCUS       DBNGSX010000263         3426 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_991633, whole genome shotgun sequence.
ACCESSION   DBNGSX010000263 DBNGSX010000000
VERSION     DBNGSX010000263.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_991633"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..1377
                     /locus_tag="AC16CB_07995"
     CDS             220..1377
                     /locus_tag="AC16CB_07995"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR3342448.1"
                     /translation="MTYLTHLECTQCQNRFNAHKVHTFCPECTAPLFARYDLEKAREE
                     LDRERIQRSPRGMWRWKDLLPVFEPSNIVSLGEGDTPLLNIPRLGEQLALPHLYVKDE
                     SLNPTGTFKARGLSAAVSKANEYGLNKVIIPTAGNAGGAMAAYAARGGLQACILMPKD
                     TPRANIEECRASGAQVILIDGLISDAARMANEIAKAEDWFDLSTFKEPYRVEGKKVMG
                     YELAEAFGWQLPDVIIYPTGGGTGLVGMQKAFSELENLGWLEKATKPRLVAVQAEGCA
                     PVVKAFNDGATVCEFWENAHTLASGLRVPKSFADRLILQDIHASDGYAIAVSDEQILQ
                     AQRQLAVTEGIFAAPEGAATLAALSILLEKGLIQPEGRIVLFNTGSGLKYI"
     gene            complement(1387..2241)
                     /locus_tag="AC16CB_08000"
     CDS             complement(1387..2241)
                     /locus_tag="AC16CB_08000"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR3342449.1"
                     /translation="MQTVISYKEKKVIIAPEQPFVIIGERINPTRRKKLAEGMSSGDF
                     SLVQQDALQQVEAGANVLDVNAGIPGGDEPALLRGAAKAVMEVVNVPLCIDTANPDAL
                     EAALKDYPGKALINSTTAEAKMMERVFPLAKQYGAAVIGVITDESGVPATPEDRLRIA
                     ERLVKRAGEYGIPAEDILIDCLALTVGADHKAGKVTLDAMQLIRKELGVNLSLGASNV
                     SFGLPDRKIINVAYLALAIGRGLTAAITDPTVPEIQSTLLACDLLMGRDDYAMRWIKA
                     YRKRAKEA"
     gene            complement(2524..>3426)
                     /locus_tag="AC16CB_08005"
     CDS             complement(2524..>3426)
                     /locus_tag="AC16CB_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342450.1"
                     /translation="GHGGHEPVLLLEPFVLVGAGVIPGEYGDVNMVDVAPTLSVLVGA
                     NIPAATQGHVLEEMLTLSEVHKNALPSVTYDQQKQLLEAYQRAIGVEIEQAPPMKTSA
                     GQVVDDFQAALEATRWDRLRSERLRRAVIASALFLLPLVFLIWKRGKDVAFMLVGALF
                     YHVLFHFRYATLDRLPYSLSWVDSQEALIQYIGVTAAIALGLVWSVIWLALKAWLRKP
                     RQAAEFGLGLTLTVIYSLSLPPLWSFVLNGAFVTWTLPDFPSLYLGFISVLQILVVAV
                     LGLAETGIAALISRFAHIETVRPS"
BASE COUNT          889 a          910 c          851 g          776 t
ORIGIN      
        1 gattgcaaca gggtcaggag agcgggcctg gtcattatac gccaaattac cctatgaggg
       61 tatacacgag ctgaagatta aagccaaggt ttcagccaac gagttctaag agaatatcat
      121 tgtaacctac cacaaaagga tataatcatc tcactttata ctgagtggag ataaagtctc
      181 ccatatcatc tgcatttcgc aggttttatt tattgatata tgacctatct gacccaccta
      241 gaatgcacgc agtgccagaa ccggtttaac gctcacaaag tgcatacctt ttgtcccgag
      301 tgcacagcac cgctatttgc tcgctatgat ctagaaaagg cacgcgagga gctggaccgt
      361 gagcgcatcc agcgctcgcc gcgaggcatg tggcggtgga aggatctctt gccggtattc
      421 gagccttcaa atattgtcag cttgggagag ggggacaccc ccctgctcaa tatacccagg
      481 ctgggtgagc aactcgcact cccccatcta tacgtgaaag acgagagcct gaatcctacg
      541 gggacgttca aagcccgcgg tctttcagcg gcggtatcca aagccaacga gtatggactg
      601 aataaagtca tcatccccac tgctggcaac gcaggagggg caatggcggc ttatgcggcg
      661 agaggcgggc tgcaagcctg tatcctgatg ccaaaagata ctccacgggc gaacatcgaa
      721 gaatgccgcg caagtggagc tcaggtgata ttgattgatg gtctaataag tgatgctgcc
      781 cgcatggcaa atgaaatagc caaggcagaa gactggtttg atttatctac tttcaaagag
      841 ccatatcgag tggaaggaaa aaaggtgatg ggttacgagc ttgccgaggc ctttggctgg
      901 cagttgccag acgttatcat ctacccaacc ggagggggta ccggcttggt gggcatgcaa
      961 aaagccttct ctgagctcga aaacctgggc tggctggaaa aggctaccaa gccacgtctg
     1021 gtggctgtgc aggcagaggg atgcgccccc gtagtaaaag cctttaatga tggggcaaca
     1081 gtttgcgaat tttgggaaaa tgctcatacg ctggcctccg gtctgcgagt accgaaaagc
     1141 ttcgctgatc gtttaatact gcaagacatc catgctagcg atgggtatgc aatcgccgta
     1201 agcgatgagc agattttaca agctcagcga caactggccg tcactgaagg gatattcgcc
     1261 gctccagaag gagcagcaac cctcgccgcg ctgagcatac tgctagaaaa gggcttgatc
     1321 caacctgagg ggcggatcgt cttatttaat accggcagcg ggttgaaata tatctgagag
     1381 ccagaattaa gcctctttag cacgcttacg atatgcctta atccatcgca tggcgtaatc
     1441 atctcttccc atcaacaggt cacacgccag caaggtggac tgtatctcgg gcacagttgg
     1501 gtcggtgatg gcagccgtca ggccacgccc aattgcaagc gctaaataag ccacattgat
     1561 aatcttacgg tctggcaatc caaaggatac gttgctggca cccaggctca aattgacgcc
     1621 caactccttc cgaatcaatt gcatcgcatc tagggtaacc tttccggctt tatgatccgc
     1681 acctactgtt agcgccaggc aatcaatgag tatgtcttcc gcagggatac catactcgcc
     1741 agctcgcttt accaaccttt ccgcaatccg caagcgatct tccggtgtgg ccggaacgcc
     1801 gctttcatcg gtaattaccc caattaccgc cgcaccatat tgcttcgcca aggggaagac
     1861 gcgttccatc atcttggcct ccgcagtcgt ggagtttatc aatgcctttc ccgggtaatc
     1921 ctttaaggca gcttccaagg cgtcagggtt ggctgtgtcg atacacagcg gcacattgac
     1981 cacctccatg acggccttcg cggcacccct cagcagtgca ggttcgtcgc cgcctggaat
     2041 gccggcattg acatccagta cgttcgctcc agcttccact tgctggagcg catcctgttg
     2101 gaccaatgag aaatcgccag acgacatgcc ttcggctagt tttttccgcc gggtgggatt
     2161 gatacgctcc ccgataatga caaaaggctg ttcaggtgca ataatgactt ttttctcttt
     2221 gtatgagatc acggtctgca tgatcatctc cctaggtatg acaaaattta tagctctaat
     2281 tggcgcgtta tgcttggagc atcaggtgca aaaccatacg ctctaatgcc tgcatcaaaa
     2341 tcatctctta accagcagca cactgatcat tgaacgaccg gcaggtatgc tcgccatgtc
     2401 ccagacagat cagcaaggaa aatccgtagc gttgctggcg tttgcttatg tatttctaag
     2461 gcgtgatcga gttgaaattt cattttatac ttccaatttt atatcaattg cttgagacca
     2521 attttacgaa gggcggactg tctcaatatg ggcaaatctg gaaatcaaag cggcaatgcc
     2581 agtttctgct aaccctaata cagctaccac caagatttgt aataccgata tgaagcccaa
     2641 atacaagcta gggaaatctg gaagtgtcca ggtcacgaac gctccattca agacaaaact
     2701 ccacagaggc ggcagagaga ggctgtagat cacggtcaaa gtcagtccta agccaaattc
     2761 tgcagcctgc cgtggctttc gcaaccaggc tttcaaggcc agccatatca cagaccacac
     2821 caaccccagc gcgatggcgg cagttacgcc aatatactgg attaaggctt cctggctatc
     2881 cacccagcta agcgaatagg gcaggcggtc taaagttgca tagcggaagt gaaacaagac
     2941 gtggtaaaac aacgccccaa cgagcataaa ggcgacatct ttgccgcgtt tccagatcaa
     3001 aaataccaat gggagcagaa agagggcact ggctatgacg gcgcgcctca gacgctcaga
     3061 cctgagtcta tcccatcggg tggcctccaa cgcagcctgg aagtcatcta caacctgtcc
     3121 agcggaggtc ttcatgggag gcgcttgctc gatttctaca ccaattgcgc gctgatatgc
     3181 ctcaagcagc tgcttttgct ggtcatatgt taccgatggc agggcgtttt tatggacctc
     3241 gctgagtgtc aacatctcct ccaacacatg cccttgagtc gcagccggta tgtttgcccc
     3301 aacaaggaca gagagtgtag gcgcaacatc caccatgttg acatcgccat attccccagg
     3361 aatgacaccc gccccaacca aaacaaacgg ctcgagcagc aaaactggct catgcccacc
     3421 atgacc
//
LOCUS       DBNGSX010000264        15097 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1060577, whole genome shotgun sequence.
ACCESSION   DBNGSX010000264 DBNGSX010000000
VERSION     DBNGSX010000264.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 15097)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15097)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15097
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1060577"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="AC16CB_08010"
     CDS             <1..513
                     /locus_tag="AC16CB_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342451.1"
                     /translation="HAEGNSIFISINPSWDHERAPSGHRAVTISTHTNLTPWWQIYHE
                     DKAQYESRKNEYADKVMSNAEIALPGFRRAAKLILPGTPITFQRFTRRIDGWVGGFPQ
                     THLLRTLSPKIGKYMWMVGDSIFPGQSTAATALGGLRVARHILDQLEYKVYLSSSQKS
                     LALARTSHSD"
     gene            563..1462
                     /locus_tag="AC16CB_08015"
     CDS             563..1462
                     /locus_tag="AC16CB_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3342452.1"
                     /translation="MGIGTWAWGDRIYWGYGRNYSEPEVRTAFETSLNAGINFFDTAE
                     IYGQGHSERILGQFIREMSSSEDVNLIIATKFYPYPWRIWSKSLKFAIEGSLKRLQID
                     SIDLYQIHYPYPPVSIETWASALADTVEDGLARTVGVSNYNSSQMRRAHTVLVKRDVL
                     LASNQVNYSLLNRKIEKNGLLKLCHELGVSCIAYSPLAQGALTGKYSPQKPLSGIRGR
                     IYSAKLLSQAEPLLRLMREIGRAHDGKTLSQVALNWVICKGAIPIPGVKNHQQTQENI
                     GALGWRLRVDEVLALDQASDRLG"
     gene            1478..2605
                     /locus_tag="AC16CB_08020"
     CDS             1478..2605
                     /locus_tag="AC16CB_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342453.1"
                     /translation="MYTIEKIDTNNKTQVREFVHFPYRLYAGNQQWVPPLFIDAETQL
                     NRKKHPFYEHSDADFFLAFRDGRIVGRVAALENGRFNEYHGTRQAQFYLFDSENDPMA
                     ASALLDRVFEWAHQRKLDTIIGPKGFGVLDGYGLLIEGYEHRQMMTMMNYNHAYYIDL
                     IEANGFHKEVDFISCHLDAKIFRLPERIHRIADRVQQRGTLKVSRFRTKRDLRAWADR
                     IGQAYNKSFINNWEYYPLTDREIAFLLENLLTIADPRLIKIITHDEEAVGFLFGFPDL
                     SAALQRAKGRLLPFGLIDLLLEMRRTKWIALNGAGILPEFQGRGGNALLYSEMEKTIR
                     DYDFLHADLTQVAETAVQMRNDLVNIGGKPYKNHRVYIRHI"
     gene            complement(2644..4113)
                     /locus_tag="AC16CB_08025"
     CDS             complement(2644..4113)
                     /locus_tag="AC16CB_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3342454.1"
                     /translation="MWNLTLRSPEGKPREFQISKSKTTIGRRSGNDIIVADISASRIH
                     AVIEHHDEQDSLIIRDLGSTNGTFVNRERLVKPRYLRHQDVIRIGEHVINVVKNQVGS
                     NVRAGSVTRQLTRDVVLESIDQHAVLIYEISRKLNNVLNIDSALKEVSNMMRQALGAD
                     RCVVMLVDQFEKLSQMGFPNSIADLVIKQRSAIIIPDLVEETDLYGESASLNRVRSVL
                     CVPVVSGEEMLGLIYMYKTDPFERPFDQRDFQLAVAISHQAALTIQRMYLLERFQEEQ
                     HTRELLLRFVSPAEADFILKEYVPSGRLPDLAEHVLTVLFADIADSTGMAERLGAKDF
                     GAVLTRYYQDMTEVVFEHGGMVDKFLGDGIMAIFGMGGNRVDDPEGRAVTAGIDMLDM
                     LDKINNEICEPIRIGIGINTGLVVAGYVGTKQQVELTVMGDSVNVAAGLQQMARPNRL
                     YVGPATMAVIAGRFKSRRVGAMVMKGRLGKIQAHEILRN"
     gene            4369..5655
                     /locus_tag="AC16CB_08030"
     CDS             4369..5655
                     /locus_tag="AC16CB_08030"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3342455.1"
                     /translation="MPDNKNLKQLLAEPHKMEDLHIPQNIVIDIILRLLFNEGNVALG
                     RFVDVLKVSNRIIDDVMLWMQKEHLVEVSKASSELGRLGYVYTLTDAGKQRARDALDR
                     SLYVGPVPVPIHYYNRGMELQTKQRRRIPADQVKEALKPLVLPPDFHRRIGPAINSAS
                     SLFLYGPPGNGKTTIAQGIGNLIAGVEPIWIPYALTAGGHIIQIHDRLIHKSITIEGG
                     RTEELGRVDQRWGLFTRPSVIVGGELKMEALDLRFDPVAKIYEAPLQLKANGGMFLLD
                     DFGRQQVRPSDLLNRWIVPLENEVDFLRLLSGQTIVVPFRMLIVFSTNLDPNELVDDA
                     FLRRIQIKVEVVAPDEKLFYQIFMNMCKYFNIPFERESFVYLLQNWYRQNGRVMQAVH
                     PRDIIRTIIALCDYEGIQPKLTPELIDDACRTYFVK"
     gene            5677..6861
                     /locus_tag="AC16CB_08035"
     CDS             5677..6861
                     /locus_tag="AC16CB_08035"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3342456.1"
                     /translation="MPSIVQEKINQAINILRDKNVDLWLTFVRETTASGDPVLPLIYG
                     GNLTWQSALLLTKSGERIAIAGRFEAEAIRNTGAYTEIIPYDEAISPILRATLERLNP
                     EKIAVNFSRNDVLADGLSHGLYQVLCDHLSGTPFVERFMSAEKVIASLRGRKTEGEIA
                     RIRSAIEITDQIFTNTFDYAQVGMTENQISEFMHAQLDQFSLEPAWDLDHCPVVNAGP
                     DSVAGHVGPSQLAIKPGQILHIDFGVKKDDYCSDIQRLVYFLAPGEQKPPAEVQRGFE
                     TILQSTQAAINAMRPGITGKEVDAVAREIITDAGYPEFKHATGHHLGRLAHDGAGTIG
                     PLWERYGDTPNFQLEAGQVYTIEPSLYLPGYGHMGIEEDVLVTENGAEYLTVPQRELI
                     LK"
     gene            6946..7989
                     /locus_tag="AC16CB_08040"
     CDS             6946..7989
                     /locus_tag="AC16CB_08040"
                     /inference="COORDINATES: protein motif:HMM:NF024585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR3342457.1"
                     /translation="MCEFCTEHGEGKAWYLTMKNYSQELLSQNGRIEHIRAFFKDFEI
                     RTAQSLSMLDQIQALPFVPDIVSRVVTSRQKKAHFGQVVPIEDVDRLLDEISSVVRIP
                     CVCRSLTTGRQETRYCYGLGIDPTGLIGAYPDYGENLEWLPREEARSAIHKLDQQGLV
                     HSVWTFDTPFIGGLCNCDQDCIAYRLQIGTGMVQVFFPAEHVASIDWDDCTGCKLCRG
                     YCSFGAIRYTSLHDKCLIDPNLCYGCGVCRATCKKDAIHLEQRKRTFRWQRKISQPGQ
                     HRVLVNGCQNARQCRACIRVCPSQVFVIAPQEGRSEGQRATDWVARAVLPSRCTDCRE
                     CITACPANAIVVN"
     gene            8001..8972
                     /locus_tag="AC16CB_08045"
     CDS             8001..8972
                     /locus_tag="AC16CB_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3342458.1"
                     /translation="MEYVRLGRTGLKVSRICLGTMNFGPYTTEADSYAIMDHALELGI
                     NFFDTANVYGRKIGEGVTEQIIGRWLAQGGGRRNQIVLASKVYGRMGEGMNERRLSAY
                     HIRQAVEGSLQRLKTDHIDLYQMHHVDRETPWEEIWQAMEILVQQGKVLYVGSSNFAA
                     LHIAQAQCAAEKRHFLGLVSEQSLYNLTARMIELEVIPACQSYGLGLLPWSPLAGGIL
                     GGSPDEQNRIRRSSENAQKDLSKHRQNIDAFEKVCKEIGETPANLALAWLLNNPAVTA
                     PIIGPRTIEQLNNIQRATEIKLSDELLGKLDEIWPGPGGAAPEAYAW"
     gene            8990..9892
                     /locus_tag="AC16CB_08050"
     CDS             8990..9892
                     /locus_tag="AC16CB_08050"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR3342459.1"
                     /translation="MNSRKYLKPGLAKRFIMVALRILFDNPIGLALAGASRVFYPRNG
                     SQWVEVNHIPLRLPRLSPKFHGYRLVQISDFHLGTWLNYNDLRHAVELVNDQNPDLVV
                     ITGDFVTINSQKYYSDLVAALGILHATDGVVAVLGNHDHWSDSAEIIRTLNQINIKIL
                     CNDVFTLKRGKEQLHICGIDDMLVGRDHLDQVLLMLPEDGAAILLAHEPDFADISAAT
                     RRFDLQLSGHSHGGQIVLPWIGIPFLPSYASKYVSGLYRVKDMYQYTNRGLGTSELQI
                     RVNCPPEISVFTLEAVKLPKHDSS"
     gene            9938..11098
                     /locus_tag="AC16CB_08055"
     CDS             9938..11098
                     /locus_tag="AC16CB_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HIR3342460.1"
                     /translation="MREVAIIGIGQTPVGEHWEKSLRELAGEAIVAAMQDANRERVDG
                     LFIGNMLSGMVNHQENLGSLIADWVGLQGIEAFKVEAACGSGAAALRVAIMAVASGEM
                     ESAIALGVEKMTDNKGTDTTAALATAADADYEAAMGLSFVAINALIMQRYMFEFGWKH
                     DDFAPFSINAHANAQHNPYARLHETIDATDYQQVRMVADPINLLDASPIGDGAAAVLL
                     IPAPHVHTNGKGWPAITIAGSAISTDTLAVHDRHDPLWLSAASRSARIAYAQAGIGPN
                     EIDLFELHDAFSIMSALSLEACGFAERGQGPRLALNGDITLTGRIPVTTLGGLKARGH
                     PVGATGIYQIAEVVQQLRGEAGKAQLGKARIGMAQNIGGSGATAITHILKVK"
     gene            11597..12451
                     /gene="panB"
                     /locus_tag="AC16CB_08060"
     CDS             11597..12451
                     /gene="panB"
                     /locus_tag="AC16CB_08060"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIR3342461.1"
                     /translation="MTERKKITLPYLFKKVADGKPITWLTCYDYPTAYLQEQAGIDMI
                     LVGDSLGMTMLGYESTLPVTMEDMIRHSQAVRRGAPNVFLIGDMPYMSYQPSTEMAIR
                     NAGRFMAEAACDAIKLEGGAEMADRVHGIVSAGIPAIGHLGLTPQSVSALGGFRVQGK
                     GALQAKKIIDDAKALQDAGAFAILLEMVPDRVCQLITQRAKDCIILSLGSGLQAHGQL
                     LIYHDMFGLYPRFKPRMAKVYGDAGQLILDGLTQYVEEVSAKTFPQPENWFGMPDEEY
                     EELVQMLK"
     gene            12500..13765
                     /locus_tag="AC16CB_08065"
     CDS             12500..13765
                     /locus_tag="AC16CB_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342462.1"
                     /translation="MPQDLRQTLRIPHARLDEINSLLLDQNSRVVNDLLEVVSRYGTP
                     EEINQKADEARQFPNLLKRIKETNPKYLADLEWLETQRDLGSFINVADYRRNVLGARA
                     DELHFKDDFAVTLEISAAQYFPWIIAIANRSIENGMLMPGRFIKVRKMREQENDGDLP
                     AIAAAMQIIGASYVETLDTKGTDGSNVHLGGPETITGYFGGVGQPNDYPLKWLDEFLY
                     YYTNYGVKQVLNINPGTVLVGYLLHKLGVDIEFKISVFMGNDNPYAALWTLLGAKLFS
                     RDDGTSPLIGFNWSNSVNNQTMEITAEFRKGLGFEEVVRFEHHITETWKSIVRQPYNR
                     RQELVEIADHVANISAKHEGGDPEIDSKRVHPSDILDYFRDRSEVIASGDWDCLLLNF
                     IDKFDALNHTARALTENGLSFVAAQNLHK"
     gene            13777..>15097
                     /locus_tag="AC16CB_08070"
     CDS             13777..>15097
                     /locus_tag="AC16CB_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3342463.1"
                     /translation="MKSLLEKLNIKDVNPGACTGSDGWITDPNGKELVSYNPATGEPI
                     AKVVQATPETYETIASTAYNSFLAWREIPAPKRGELVRDLGIALREYKEPLGDLVTIE
                     MGKIRVEGHGEVQEMIDIADFAVGLSRQLYGLSMHSERPQHRMYEQWHPAGVLGLITS
                     FNFPTAVWSWNSTLAAVCGDTVVWKPSSYTPLTAIATQHISNRVMAEHGYSGIFNLVI
                     GSGRDVGELLLNDPRMPVVSFTGSTSIGRHVSEAVARRFGRTILELGGNNGISVAPDA
                     DLDLATRAILFGAVGTAGQRCTSTRRIIMHKDISENLSNRLVNAYKQVKIGDPMDPSV
                     LMGPLVVNTSVEEMMDAISQAKAAGGQVLAGSNMLPDLGPNFVEPTIIKMPAQTEIVK
                     KETFAPILYLLEYETMDEAVQLHNGVPQGLSSAIFTDSMRNVEHFLSV"
BASE COUNT         4116 a         3665 c         3556 g         3760 t
ORIGIN      
        1 cacgcagaag gtaattccat atttatttca ataaatccaa gctgggatca cgagcgggcg
       61 ccttcaggtc accgagcggt gaccataagc acccatacta atttaacccc atggtggcag
      121 atttaccatg aggataaggc acaatacgaa tccaggaaaa atgaatacgc cgataaagtt
      181 atgtctaacg ccgaaatagc tcttccgggc ttccgccggg ctgctaaatt gattctacct
      241 ggcacaccaa ttacatttca acgctttacc cgccgtatcg atggttgggt aggtgggttt
      301 ccccaaaccc acctgctgcg tacccttagc ccgaagatag ggaaatacat gtggatggtt
      361 ggtgatagca tcttccccgg gcaatccact gctgccactg ccctaggagg gcttcgagtt
      421 gcacggcaca tcctggacca attggaatac aaggtttatc tttctagttc tcaaaaatca
      481 ttggcactgg caaggacttc tcatagtgat tgatagtcaa gatcgaattg cattaggaaa
      541 aacagacatt atgatctctc caatggggat aggaacatgg gcatggggag atcgaattta
      601 ttggggatat gggcgcaact attctgaacc agaagtaagg accgcatttg agacaagcct
      661 gaatgctggt attaattttt ttgataccgc agagatatat ggtcaaggcc attctgaaag
      721 aatattaggc cagtttattc gcgaaatgtc ctcaagtgaa gacgtaaatc taatcattgc
      781 cacaaaattc tatccgtatc cttggcgaat ctggagcaag agcttgaagt tcgctatcga
      841 gggcagctta aaacggctgc aaatagatag tatcgatctc taccaaattc attatccata
      901 tccccccgtt tccatagaaa catgggcatc agccttggca gatacggttg aggacggcct
      961 tgcgcggacg gttggtgtat ctaattacaa ttcgagccaa atgcgccggg ctcatacggt
     1021 tttggtaaaa agggacgtac tgctggcttc caatcaggtc aactacagct tgctcaaccg
     1081 aaaaatcgaa aagaacggct tattaaagtt atgccatgaa ttaggggtat cttgcattgc
     1141 ctacagcccc ctggctcaag gggcgttaac tggcaagtat agccctcaaa aacccctgtc
     1201 gggtattaga gggcgtatct actctgccaa attactatcc caagcagaac ccttgctgcg
     1261 cctaatgcgc gagattggtc gcgctcatga tggtaaaacc ctaagccagg tagcactaaa
     1321 ttgggtcatt tgcaaaggcg ccatcccaat tccaggcgta aagaaccacc agcaaaccca
     1381 ggaaaacatt ggagccttgg gttggagatt aagagttgac gaagtgttgg cattagacca
     1441 agccagcgat cgtttaggtt gaactggaga gggcaaaatg tacacaatcg aaaaaattga
     1501 caccaataat aaaacccaag ttcgagagtt tgtacatttt ccttatcgtt tatatgctgg
     1561 gaaccagcaa tgggttccgc cactttttat tgacgcggaa acccagctca accggaagaa
     1621 acatccattt tacgagcatt cagatgccga ttttttctta gcttttcgag atgggcgcat
     1681 tgtaggccgt gtcgctgctc tggagaacgg gcgttttaat gaatatcacg gtactcgcca
     1741 agcacagttt tacttgtttg actctgagaa cgatccgatg gctgcaagcg cattgcttga
     1801 tagggttttc gagtgggcgc accaacgcaa gctcgacacc attattggcc cgaagggttt
     1861 tggggtactc gacggttatg gtctattaat cgaaggctac gaacatcgtc agatgatgac
     1921 gatgatgaat tacaaccacg catactacat agatctcata gaggcgaatg gatttcataa
     1981 agaagtggat tttatctcat gccatcttga tgcaaagatt tttcgcctgc ccgagcgcat
     2041 ccataggatc gccgatcgcg tccagcaacg aggcacactc aaagttagtc gttttcgcac
     2101 caaacgcgat ttgagagctt gggcagatcg gattggtcaa gcatacaata aatctttcat
     2161 taacaattgg gagtattatc ccctcaccga tcgagagatc gcgttcttgc tcgaaaatct
     2221 attaactatt gctgatccgc gcctgattaa gatcatcacc catgatgaag aagcagtagg
     2281 gtttctattc ggcttcccag atttgtcagc agccttgcaa cgcgccaaag gacggctact
     2341 accttttggt ctaatagatt tgctactcga gatgcggcgc acgaaatgga tagcgctaaa
     2401 cggagcgggc atcctgccag aatttcaagg tcgcgggggc aatgccctgc tctattctga
     2461 aatggaaaag accatccgcg attacgattt ccttcacgca gacttgacac aggtagcaga
     2521 aactgcagtg caaatgcgaa atgacctggt gaatataggc gggaagcctt ataaaaatca
     2581 ccgcgtatat atccgccata tttgatagag cataccgctc ggctatctcg agcggttttt
     2641 ctattagtta cgcaatatct cgtgcgcctg tattttgcct aatcgacctt tcatcaccat
     2701 tgctccaacg cgtcgcgatt tgaaacgacc agctatgact gccatagttg ccgggccaac
     2761 atacagccga ttaggccttg ccatttgctg caagccagca gcgacattaa ctgaatcgcc
     2821 catcacggta agctccactt gttgcttggt gccaacatac ccggcgacaa ccagacctgt
     2881 attaattcca attcctattc taattggctc acatatctca ttgttgattt tatcaagcat
     2941 gtctagcata tctattccag cagtaacagc ccggccttcc gggtcatcta ccctattgcc
     3001 gcccatccca aaaatggcca taattccatc tcccaggaac ttatccacca tgcctccatg
     3061 ctcaaagacg acctcggtca tatcctgata ataacgggtg agaacagcac caaaatcctt
     3121 agcacctaac ctttcagcca ttccagtcga atcagcgata tccgcaaata ataccgtcaa
     3181 cacatgttct gccagatctg gcaaacggcc tgatggcaca tattctttta gtataaagtc
     3241 ggcctcagcc ggagaaacaa atcgcaataa cagctctcgc gtgtgctgtt cctcttgaaa
     3301 acgctctaag agatacattc gctggatagt tagtgcagcc tgatggctta tggcaactgc
     3361 caattggaag tctcgctgat caaatggacg ttcgaacgga tcagtcttgt acatgtagat
     3421 caatcccagc atctcttcac cactcaccac tggcacacac aagactgaac gaaccctatt
     3481 taatgaagcg ctttcgccat aaagatctgt ctcttcaact aaatcgggaa taatgattgc
     3541 agatctttgc ttaatcacca aatcggcgat tgaattggga aagcccattt gcgaaagctt
     3601 ttcaaattga tctaccagca tcacaacgca ccgatcagcc cccaaagcct gtctcatcat
     3661 gttagatact tctttgagag cggaatcaat attcaacaca ttgttgagct ttctagaaat
     3721 ttcatagatc aagacagcat gttggtcaat cgattcgaga actacatctc gcgtgagttg
     3781 tcgtgtaact gatccagcac gaacattgct gccaacttga ttttttacta cattgataac
     3841 atgttcgcct atacgaatga catcctgatg gcgtagatac cgcggcttta ctagccgctc
     3901 acgattgaca aatgtgccat tggtactacc aagatctcga atgatcagag agtcttgttc
     3961 gtcatgatgc tcgatcacag cgtgaattcg cgacgcagaa atgtccgcta caattatgtc
     4021 attacctgat ctgcgcccaa tagtcgtttt tgatttagaa atttgaaatt cacgcggttt
     4081 tccttctgga gaacgcaaag ttaaattcca cattgtatgc taaagccctc tttcctgcgc
     4141 gtcaatcacc tagaaatcaa tagcacatca tttcaatttt attaccaaac aagatatatt
     4201 ttttcttgaa catgagaact ttctatattg ttagcgcagc aattccatgg cttgctacca
     4261 tcggctggat ggtaaaattc aagggggata ggtgccaatc cttgcacctc tttatccagg
     4321 ttatcataaa actacagaag gcatactccc gaacacggaa ggtaacgaat gccagataat
     4381 aagaacttga agcaacttct agccgaacct cataaaatgg aggacctgca tatcccccaa
     4441 aacatcgtga tagacattat tttacgttta ctatttaacg aaggcaatgt agcactaggt
     4501 agatttgttg acgtacttaa ggtgtccaac cggattatcg atgatgtaat gctgtggatg
     4561 caaaaagagc atctggtcga agtatccaag gcgagttccg aattaggtcg tctaggatat
     4621 gtttatacac tcaccgacgc aggaaaacag cgtgcccgcg acgctctgga tcgcagtctt
     4681 tacgtaggcc cagtacctgt cccaattcat tattacaacc gtgggatgga gctccaaacc
     4741 aagcaacgca ggcgcatacc cgcagatcag gtgaaagaag ccctgaaacc actcgtctta
     4801 ccccccgatt ttcaccgccg gattggccct gccataaact ctgcctcatc attgttcctg
     4861 tatggtcccc caggaaacgg caagacaact atagcccagg gtattggcaa tttgatcgcc
     4921 ggcgtggagc caatctggat accttatgct ctgaccgctg gtgggcacat cattcaaatt
     4981 cacgaccgcc tgatccataa atcgattacc attgaaggcg gacggactga agaattaggg
     5041 cgtgtagatc agcggtgggg tttatttacc cgcccatcag taatagtcgg tggtgagcta
     5101 aaaatggaag cgctggatct acgctttgac ccggtagcaa aaatatacga agcaccgcta
     5161 cagctcaaag cgaatggagg catgtttctc cttgatgatt ttgggcgtca gcaagtacgc
     5221 ccatcagacc tacttaatcg ctggatcgta ccgcttgaaa acgaggtcga ctttctccgc
     5281 ttgcttagcg ggcagacaat cgtagtcccc ttccgtatgc tcatcgtatt cagcaccaat
     5341 ctagacccaa atgaattggt ggatgatgcc ttcttgcgac gaattcaaat caaagtagag
     5401 gttgtcgcgc cagatgaaaa gcttttctat cagatattca tgaatatgtg taagtatttt
     5461 aatattcctt ttgaacgtga atctttcgta tatcttctcc aaaattggta caggcaaaat
     5521 ggtcgggtca tgcaagctgt acacccaagg gatatcatcc gaaccatcat cgcactttgc
     5581 gattacgaag gcatacaacc aaaattaact ccagaattaa ttgacgatgc ctgcagaaca
     5641 tattttgtaa aataagtcgg cgccaggagg taagagatgc ctagtatcgt acaggaaaaa
     5701 attaaccagg cgatcaatat tttacgcgat aaaaatgtag atctatggct cacttttgtc
     5761 cgcgagacga ccgcatccgg tgaccctgta ttaccgctca tctatggagg taacctgacc
     5821 tggcaaagtg cacttttgtt gactaagtca ggggaaagga tcgccattgc aggccgattt
     5881 gaagctgagg ccataagaaa taccggagct tatacagaaa tcatcccata tgatgaagcg
     5941 ataagcccca tattacgagc gactctggag cgtcttaatc ctgaaaaaat tgcagtcaat
     6001 ttctcccgca atgatgtcct tgctgatggt ttatcccatg gtctttatca agtattgtgc
     6061 gaccacctct caggtacacc atttgtcgaa agatttatga gtgcagaaaa ggtgatcgcg
     6121 agccttagag ggcgaaaaac tgaaggagaa atcgctcgta tccgctctgc tatcgagata
     6181 actgatcaaa tattcacgaa taccttcgac tatgcgcaag taggcatgac cgaaaatcag
     6241 attagtgaat ttatgcatgc tcaactcgat caattcagcc ttgagcccgc ctgggatcta
     6301 gatcattgcc cggttgtaaa cgctggacca gactccgtgg cagggcacgt cgggccatca
     6361 caactcgcga ttaagccagg gcagattttg cacattgatt ttggggtaaa gaaagatgat
     6421 tattgctccg atattcaacg cctggtttat ttcctcgctc cgggtgaaca aaaaccacct
     6481 gcagaagtac aacggggttt tgaaacaatc ctccaatcca cacaagccgc tattaatgcc
     6541 atgcgcccag gcatcaccgg taaagaggta gatgctgtag ctcgggaaat catcaccgat
     6601 gcaggttatc ccgagtttaa acacgccaca ggtcaccacc tgggcaggct agcccacgat
     6661 ggcgcaggca ccattggccc tctttgggaa agatatggcg atacacccaa tttccaattg
     6721 gaagctggtc aggtttacac cattgaacct agtttatacc ttcccgggta tggacacatg
     6781 ggtattgaag aggacgttct ggtcactgaa aatggggctg agtatctaac agtccctcag
     6841 cgtgagttaa tcctcaagta atctttttat cgttagcaat ctcctgagga tttttgatat
     6901 cctcgtttac aataatatcg cctaacttta ttccaggagg cggatatgtg tgaattttgt
     6961 accgaacacg gcgagggcaa agcatggtac cttacgatga aaaactatag ccaggaattg
     7021 ctttcgcaaa atggcagaat cgaacatatt cgcgcatttt tcaaagattt tgaaatccgc
     7081 acagcgcaat ccctctcgat gctcgaccag attcaagccc ttccttttgt gcccgatatt
     7141 gtaagccggg ttgtcacatc acgccaaaaa aaggcccact ttggtcaagt cgtaccgatc
     7201 gaagatgtgg atcgcttatt agatgaaata tcgtccgttg ttcgcattcc ttgtgtctgc
     7261 cgcagcttga cgacaggcag gcaggaaacg cgctattgct acggattagg cattgatccc
     7321 acaggcttga tcggtgcata tcctgattat ggcgaaaatc tggagtggct accgcgcgag
     7381 gaagctcgca gcgcaatcca caaattggat cagcaagggc tagttcactc cgtttggacc
     7441 tttgatacac cctttatcgg aggcttgtgt aattgtgatc aagactgtat tgcctataga
     7501 ttgcagattg gcacaggcat ggtgcaagta tttttcccgg ctgagcacgt ggcatccatc
     7561 gattgggatg attgcaccgg ctgtaagctt tgccgtgggt attgttcctt cggggcaata
     7621 cgctacacct ctttacacga caaatgcctg atcgatccca atttgtgcta tggatgcggt
     7681 gtatgcaggg caacctgcaa gaaggacgct atccatcttg agcaacgaaa gcggaccttc
     7741 cggtggcaac ggaaaatatc ccaaccagga cagcaccgag ttctggtaaa cggctgccag
     7801 aatgcccgtc aatgtcgagc atgtatccgg gtttgcccat ctcaggtatt cgtcattgct
     7861 ccgcaagagg gacgttctga aggacagcgc gctacggatt gggttgccag ggctgtatta
     7921 cccagccgct gtacagattg ccgtgagtgt ataacggcct gccccgcaaa cgccattgtt
     7981 gtcaattaac aaggaatatt atggaatacg ttcgtcttgg aaggactgga cttaaagtta
     8041 gtcgaatatg cttgggaacc atgaatttcg gtccctatac taccgaagct gacagctacg
     8101 ccatcatgga ccacgccctg gagctgggta taaacttctt cgatactgcc aacgtctacg
     8161 gcaggaaaat tggtgagggt gtcaccgagc agatcatcgg aagatggcta gcccagggag
     8221 gtgggcggcg gaatcaaatc gttctagcaa gcaaggttta tggtcgcatg ggcgaaggca
     8281 tgaacgagcg ccgcttgtct gcatatcaca ttcgccaggc tgtagagggt agcttacaac
     8341 ggttaaaaac tgatcatatc gatctgtatc aaatgcatca tgttgatcgg gaaacgccat
     8401 gggaagaaat ctggcaagcg atggaaatcc tcgttcagca ggggaaagtc ctttatgttg
     8461 gcagcagtaa ttttgcagcg ctgcatattg cccaggcgca gtgtgctgca gaaaaacgcc
     8521 atttcttggg tctggtgagc gagcaaagcc tgtacaatct aactgcccgc atgatcgagt
     8581 tggaggtcat cccagcttgc caatcatacg gtctagggct cttgccttgg agcccgctgg
     8641 ccggcgggat attaggcggc agtccagacg agcaaaatcg aataagacgt tcatcggaga
     8701 atgcgcaaaa agatctttcc aagcatcggc aaaatattga cgcattcgag aaagtttgta
     8761 aagaaatcgg tgaaacccct gctaatcttg cgcttgcctg gttgctaaac aatccagcgg
     8821 taacagcccc aattatcggg ccgcgcacaa ttgagcaact caacaacatc caacgtgcca
     8881 ccgagataaa gctctcagac gagctgctcg gtaagctcga tgagatttgg cctggcccag
     8941 gtggagctgc accagaagcc tatgcttggt aagtaaccgg tatcatctct tgaattccag
     9001 gaaatatctc aaaccaggct tggccaaacg ctttatcatg gtcgccctgc gtattctatt
     9061 tgataatcca attggcctag cattagctgg agcaagccgg gtattttacc cacgaaatgg
     9121 ctcacaatgg gttgaggtca atcacattcc gctacgcctt ccgcgcctct cgccaaagtt
     9181 tcacggttat cgcctggttc agatcagcga ctttcacctg gggacttggt taaactacaa
     9241 tgatctgagg cacgctgttg agcttgtgaa cgatcagaat ccggacttgg tcgtaataac
     9301 tggtgatttc gtaaccataa attcccaaaa atattactca gacctggttg ctgcactcgg
     9361 cattcttcat gcaactgatg gagtagtggc agtgcttggt aatcatgacc actggagcga
     9421 ctcagctgaa ataatccgca cacttaatca gatcaacatt aaaatcttat gtaacgatgt
     9481 atttacactt aaaagaggaa aagaacagct ccacatatgt ggaatcgacg atatgctggt
     9541 ggggcgcgat cacctagatc aggtgctttt gatgcttcca gaagacggag ctgcaatctt
     9601 gctagcccat gagccagact ttgccgatat cagcgcagca acacgccgat ttgatctcca
     9661 gctttcagga cattctcacg gtggtcaaat tgtcttgcca tggattggca tacccttcct
     9721 gcccagttat gcaagtaagt atgtctctgg tttgtatcgc gtgaaagaca tgtatcagta
     9781 tactaataga ggattgggca catccgaact tcagataaga gttaactgtc ctcctgaaat
     9841 ttccgtattc acattagagg cggtaaaatt acccaagcat gatagctctt gataggataa
     9901 aaacctacaa tattcaatgg ataaggattg tagctgaatg cgcgaggtag ctatcattgg
     9961 tatcggtcaa accccagtcg gtgagcactg ggaaaaatcc ctgcgcgagc tagcagggga
    10021 ggcaatagtc gcggctatgc aggacgccaa cagggagagg gttgatgggc tgttcattgg
    10081 aaatatgctc tctggaatgg tgaatcatca ggaaaacctg ggatctctta ttgccgattg
    10141 ggtaggattg caagggatag aagccttcaa agtagaagcc gcctgcggtt caggcgcagc
    10201 cgcactgcgc gtcgctataa tggcagtggc ctctggagaa atggaaagcg ccattgcctt
    10261 gggcgtggag aagatgactg ataataaagg cacagacact actgctgcac tcgccacggc
    10321 tgccgacgca gattatgaag ctgcaatggg attatcattt gttgccatca atgccttaat
    10381 catgcaacga tacatgtttg aatttggttg gaaacacgac gatttcgccc ccttttctat
    10441 caacgcgcat gccaacgcgc agcataaccc ttatgcccgc ttgcacgaaa cgatcgacgc
    10501 aacagactac cagcaggtgc gcatggtagc tgatcccatc aatctgttgg atgcctcacc
    10561 gattggcgat ggcgctgcag ccgtactcct aataccggcc ccccatgtgc atactaacgg
    10621 caaaggctgg ccagcaatca caatcgcagg ctcagctatt tcaacagata cgttggctgt
    10681 gcacgaccgc cacgatccgc tctggctttc agcggcttca cgctcagcac gtattgccta
    10741 tgcacaagct gggatcggtc caaatgaaat cgacttgttt gagttacacg atgcattctc
    10801 tataatgtct gccctttctt tggaagcttg cggctttgca gagcgcggtc aaggtccacg
    10861 gcttgcgctt aatggagaca tcacactcac cggtcgcatc cctgttacta cgctgggagg
    10921 tcttaaagcg cgcggccacc cagttggagc tactggcatt tatcagatcg ctgaagtagt
    10981 ccaacaactg cggggagaag cgggcaaagc ccagcttgga aaagcaagaa tcggtatggc
    11041 gcaaaacatc ggcggtagtg gcgcaactgc cataacccac atactaaagg tcaaataatt
    11101 cctgatgatg agttttttca ataagttctt tgcctcagta atatgcgcgg ttattttttt
    11161 gtccattgtg tctgcccttc ctgttggcgc acaaacgagc agaaattcat caaccaataa
    11221 acagtcaacc agcgccgagg gtaatctcaa gattacacca acacaaagct caacagcctt
    11281 catccccacc gaaacaccca agccatctaa tcaatctcgt ccaacagcca cgccgattcc
    11341 cgtccctcca ccctcagacc cacgcacaat ccaattgatg gcattatttg gcatcctaat
    11401 catggcaatc gttatatatg gcatctggat taatcggcgc aagttattct gatgtacata
    11461 aaaaggcatc cttctccaac ctatatgacc cccatcatac agatgggggt attttttcac
    11521 attgctttcg ttgcttaatt cacgtacaat aaaattacag attgcctctt tacggctgtt
    11581 caaccggagg gaagaaatga ctgaacgcaa gaagataacc ttaccatatt tgttcaaaaa
    11641 agttgccgat gggaaaccca tcacatggtt aacatgctac gattacccta ctgcatactt
    11701 acaagaacaa gctggaattg acatgatcct ggttggggat agcctgggga tgaccatgct
    11761 tggatacgag tccacgctac ccgtgaccat ggaggatatg atacgtcaca gccaggcagt
    11821 gcgccgtgga gcgccaaatg tattcctgat aggtgatatg ccatatatga gttatcagcc
    11881 aagcaccgaa atggctatcc gcaatgcagg acgctttatg gctgaagctg cttgtgatgc
    11941 gataaaactt gaaggtggag ccgaaatggc cgaccgtgtt cacggtatcg tctctgctgg
    12001 gataccagct attggtcacc taggattaac cccacaaagc gtgagtgcgt tgggagggtt
    12061 ccgtgttcaa ggaaaaggcg cactacaagc taagaagatc attgatgacg ctaaagcttt
    12121 gcaagacgca ggcgcattcg caatcctctt agagatggta ccggaccgcg tttgtcagtt
    12181 aattacccag cgggcaaaag attgcatcat cctgagtttg ggatctggct tacaagccca
    12241 cggacaactt ttgatttatc acgatatgtt tggtctttac ccgcgcttta aaccacgcat
    12301 ggccaaagtg tatggagatg ccggtcaatt gattttggat ggccttacac agtatgtcga
    12361 agaagtcagt gctaagactt tcccgcaacc cgaaaactgg tttggcatgc ccgacgagga
    12421 atatgaagag cttgtgcaaa tgctgaaatg atttttttgt gtcgacttta gcaactaagg
    12481 cttgatcagg aggtctatta tgccgcaaga tttgcgccaa acccttagaa ttccgcacgc
    12541 ccggctagac gagatcaact cgctgctgct tgaccagaat agccgtgtag tcaatgatct
    12601 cttggaagtt gtatcaagat atggtactcc tgaagagatt aaccaaaaag ccgatgaagc
    12661 ccgtcaattt cccaatttat taaagcgaat aaaagagaca aatcctaaat acctggcaga
    12721 tttggagtgg ctggaaactc aacgagatct cggctcattt attaatgttg ccgattatcg
    12781 ccgcaacgtg ctcggtgcga gagcagatga gctgcatttc aaggatgact tcgcagtgac
    12841 acttgaaatc agcgcagccc aatatttccc ttggatcatt gcaatcgcca atcggtccat
    12901 cgaaaacggc atgctcatgc ccggtcgttt catcaaggtg cgtaaaatgc gtgagcagga
    12961 aaacgacggc gatttacctg cgattgcggc tgccatgcag ataattggcg cgagctacgt
    13021 ggaaacactg gacacgaaag gcacggatgg ctccaatgta cacctaggag gtccagagac
    13081 gattacgggc tacttcgggg gtgtagggca acctaatgac tatcccctaa aatggctaga
    13141 cgaattcctg tactattaca ccaactacgg cgtgaagcag gtcttgaata tcaacccggg
    13201 cacggtactt gtcggatact tgctccataa attgggcgtg gacatcgaat tcaagatatc
    13261 cgtcttcatg ggcaacgaca acccttacgc tgctctttgg acattacttg gggccaagct
    13321 gttctcacgc gacgatggca catccccact gattggcttc aactggagca attcagtcaa
    13381 caaccagacc atggagatca ccgctgaatt ccgcaaaggc ttgggattcg aggaagtcgt
    13441 tcgtttcgaa caccacataa cagaaacttg gaaaagtatc gtacgccagc catataatcg
    13501 gcgccaagag ctggtcgaaa tcgctgatca tgtcgctaat atttctgcca agcacgaagg
    13561 cggcgacccg gaaatcgata gcaagcgcgt acatccttcg gatattcttg actacttccg
    13621 cgacagatca gaggtgattg cctccggaga ttgggattgc ctcttgctca actttatcga
    13681 taagtttgat gctctcaacc ataccgctcg cgcgctgacc gagaatggac tttcctttgt
    13741 cgctgcccag aatttgcata aataaacgag gtgtacatga aaagcttatt ggaaaaattg
    13801 aatattaagg atgtaaaccc tggcgcttgc actggctcag acgggtggat tacagatccc
    13861 aacggcaaag agttggtgtc atacaaccct gccactggag agccaatagc caaagtggtc
    13921 caagccacac ctgagaccta tgaaacaatc gcctctacag cgtataacag ctttttagcc
    13981 tggcgagaaa tacccgcgcc aaagcgcggt gagttggttc gcgatctagg tatcgcgtta
    14041 cgggaatata aggaaccgct cggcgatctg gtgactatcg aaatggggaa aatccgggtc
    14101 gaagggcacg gtgaagtaca ggaaatgatc gatatcgccg atttcgcggt cgggttatcc
    14161 cgtcagcttt acggcttaag catgcattcc gaaaggccgc aacaccgcat gtacgagcaa
    14221 tggcacccgg caggcgtgct tggcttaatc acttcattca acttcccgac tgccgtgtgg
    14281 tcctggaatt caaccctcgc tgcggtgtgt ggcgacaccg tagtatggaa accgtcttcc
    14341 tacactccat taacagcgat agcaacccaa catataagta atcgcgtgat ggctgagcat
    14401 ggctatagcg ggatatttaa ccttgttatc ggctcaggaa gagacgtggg cgagctgctc
    14461 cttaacgatc caaggatgcc agttgtgtct ttcaccggct ctaccagcat cgggcgtcat
    14521 gtctcggagg cagtagccag gcgattcggg agaacaatac ttgaactggg cggaaataac
    14581 ggcatcagtg tcgcccctga tgcagacttg gacttagcca cacgagccat cttgtttggt
    14641 gcagttggca cagccgggca gcgttgtacc tcgacacgtc gtatcattat gcacaaggat
    14701 atctcggaaa acctatccaa ccgcctggta aatgcataca agcaggtaaa gatcggtgat
    14761 cccatggatc catctgtctt gatgggacct ttggtggtca acacctcggt tgaagagatg
    14821 atggatgcca tctcacaagc aaaagctgct ggtggtcagg ttctcgccgg cagtaatatg
    14881 ctccctgatt tggggccaaa ctttgtcgaa cctaccatta taaagatgcc cgcccagacc
    14941 gaaatcgtaa aaaaagagac ctttgcaccc attctctact tgttagagta tgaaacaatg
    15001 gatgaggctg tccagctaca caatggcgtc ccccaaggtt tatccagcgc tatcttcacc
    15061 gattcgatgc gcaatgtaga gcactttcta tcagttg
//
LOCUS       DBNGSX010000265        12168 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1613947, whole genome shotgun sequence.
ACCESSION   DBNGSX010000265 DBNGSX010000000
VERSION     DBNGSX010000265.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12168)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12168)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12168
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1613947"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1174
                     /locus_tag="AC16CB_08075"
     CDS             200..1174
                     /locus_tag="AC16CB_08075"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 -
                     branched-chain-amino-acid:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HIR3342464.1"
                     /translation="MVLPINAYFKGKIVPYSEAKIGVLTHALNYGTGVFGGIRGYWNS
                     EEEQLFIFRPTDHYRRFLNSAKMMCMEINHTVESLTQITIDLLRVDGYQRDIYIRPLA
                     FKSDEIIGVKLHDLHDELSIVAVPFERYVSNDTNAHVTISSWRRVDDNAIPARGKISG
                     AYANSALVKTDAVRAGFDEALVLTQEGHVSEGSAMNIFVIRDGVVFTPPMTDNILEGI
                     TRRTAIELLKEEFGLPVVERPIDRTEVYLCDELFMTGTAAQITTVVKVDNRPLGSGVM
                     GPITTKLRKAFDDAIRGRNPRFRHWNVPVYPKPSTQEQKSTVSEKLAY"
     gene            complement(1225..2610)
                     /locus_tag="AC16CB_08080"
     CDS             complement(1225..2610)
                     /locus_tag="AC16CB_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR3342465.1"
                     /translation="MDLTFLTISQAADLISRRELSPVELAKAYFDRIRQFEPLINSYI
                     TVTEETALAAARQAEGEIQKGNYRGLLHGIPLALKDLYETAGVRTTHGSKVYADYIPE
                     SDGVVVKKLKAAGVVMLGKTNMHEIALGVTNVNPHFGPCRNPWSLERVTGGSSGGSAA
                     ALAAGMCMGSFGSDTGGSIRIPASLCGIVGLKPTFGRISLQGVIPLSWNLDHAGPMAR
                     SVRDAAILLAATAGYDPDDPASIDVPTQDYLAQLENGVRGWKIALAVDEFFSRVDDEV
                     NAALQEAAQVFKELGAQVVPVEFPEGYAAAAANGGIVTSDAAAFHIERIQTRSEDFGA
                     DVLERMQGGAARTSSEYARLRREQSRLRRVYERFFEGYDILLTATTPVTAPPIEGPNA
                     IEQARLLTRFTAPFNLTGLPALSLPCGYSSEGLPVGLQIISRPWAEASVLSAGTAYEL
                     ATQWQQRRPAL"
     gene            complement(2621..3259)
                     /locus_tag="AC16CB_08085"
     CDS             complement(2621..3259)
                     /locus_tag="AC16CB_08085"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3342466.1"
                     /translation="MNDITKDAQSPVNLRAVIFDMGGVLMRTVDQEPRRMLAARLGVS
                     LNELYDQIFSSESARLATLGKISTQEHWEQVRSHFGISPDEFPKAIQQFWDGDLLDQS
                     LLRYLGTLRERYKVALLSNAWDNMRDILENEWKIASDFDEVIISAEIGLAKPDHRIYQ
                     LTLSRLGVGADEAVFVDDFVENVDAARWLGMRAIHFRSPDQARAELERMLNT"
     gene            complement(3256..4533)
                     /locus_tag="AC16CB_08090"
     CDS             complement(3256..4533)
                     /locus_tag="AC16CB_08090"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3342467.1"
                     /translation="MSAKIEMDTRGGTSLGTQTRAPLLNNALRLFMVAMVLANIAGHM
                     YDPLLPLYLKSLNANVLQVGMFFTLAQIIPLVLLILGGWISDSLGRLRSIALGSLAGV
                     FSYLVVILAPTWQWVLLGEGFVAITRSLVAPSFGAFIAENSTEETRARVFGITETIFM
                     VVAVIGPPLGGWLVDLYGFKFMLVWSGIIYTLATVIRMSMARNAAFTKDTSPQPLSLI
                     NLRANMGTMLALLLGGGLVTWLLLADGVRDWAYALSFNLLPIYMQDIGQISVQQIGWL
                     QSIFGMAMMLVAMPAGWLADKKGERVSIALGFGFQFAALMVFIRVSSFWGFGAAWALL
                     GLGMGFMTPAFFSLMSKAVPERLRGTAFGLFSTSLGVVSLPAPVIGAQLWERFSPRTP
                     FALSAWASLLAVVPVWLKFRMTDNDQQERNGQG"
     gene            complement(4719..5546)
                     /locus_tag="AC16CB_08095"
     CDS             complement(4719..5546)
                     /locus_tag="AC16CB_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3342468.1"
                     /translation="MTTLVHEFRATYAFIERNANLVKRYWSWELVWMIYSIANSLSVS
                     FIGLGMERIGGGQNVDGRYLVLYLVIGTLVWRYLSLIFYWITEVISIERWEGTIEYTL
                     MAPIRRITHMAGQTFFAVIYSLLFTAVILVVTAVLFQLDLSDANLLGGTLMLLAGSLS
                     FIGVGVMGSILPLLFPERGAQMTHVIIALLLLVSGVYYPIEVLPLALQRLAVFSPATY
                     VLDGTRRALLEGLPTSQLWPYIWPTLLMGLLAIPAGLKVFSLAERYAKRTGKLHRNG"
     gene            complement(5573..6172)
                     /locus_tag="AC16CB_08100"
     CDS             complement(5573..6172)
                     /locus_tag="AC16CB_08100"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3342469.1"
                     /translation="MEDIFHGELVRLGSDNLQTIAEALSRWHRDSEYARLLDDEPARL
                     KSPERIKEWLEKDLEEGQHSHFFFTIRTLEEDKLIGFIGLFVTQWSHGNSWVGIGLGE
                     REYWGKGYGTDAMRLILRYAFRELNLHRVSLGVFEYNPRAMRAYEKAGFQPEGRARKV
                     VYREGLRRDIIHMGILRDEWEELMRNIDASKNGNLLRFV"
     gene            complement(6129..6737)
                     /locus_tag="AC16CB_08105"
     CDS             complement(6129..6737)
                     /locus_tag="AC16CB_08105"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3342470.1"
                     /translation="MDIKTSLFTGEQICLAPIDPDKDAEIEAIWTQNAEYMRQLNLEP
                     ARPLSPEQVKKRYTEIEKEADEKKNLFYFTIRLRSDDQLIGFARIYWIGWTSGFGLVQ
                     LGIGDATQRGKGYGTQALSLLLRYAFAELNLHSLAADIPEYNLAATRLFQNAGFVEET
                     RQRQAVYREGRRWDLIYFGILKDEWSKRNGRHLSRRTGPSWQ"
     gene            complement(6766..7638)
                     /locus_tag="AC16CB_08110"
     CDS             complement(6766..7638)
                     /locus_tag="AC16CB_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3342471.1"
                     /translation="MYAIDLQDTYKHFGKSSQPLWKRWLWNQRGSNGGVGGSISNEND
                     SKNGSKSIVVAVDHVSFYVKEGEVFGVLGPNGSGKSTLIRLISTLLLPDAGRIVVFGH
                     DVVRQPIEVQRLINRVSVEASFFKKLSPMENLLYGARLYGMEGRETRQRVVEILSRLG
                     LEERTIYKPMEEMSRGMQQKVAIARALLSRPRLLLLDEPTTGLDPRSKREVQAVVREL
                     NQEYGTTILLTTHDMLEAENLCERIAIIDGGKVVALDSPEALKRTFPRNGHEPSLEDV
                     FLELTGKKLVRESD"
     gene            complement(7926..10241)
                     /locus_tag="AC16CB_08115"
     CDS             complement(7926..10241)
                     /locus_tag="AC16CB_08115"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3342472.1"
                     /translation="MSGNKDIFQKAMDEGHSAAWDQSWERAAGFYRQALEQFPEHHQA
                     LTSLGLALFELEDYEGALRAYAKAAMVSPDDPVPLEKISQSYEQMGNLERARAASLQA
                     AELHLKKRDVDKAIENWGRVTSFQPDNFTARSRLALVYERLGKANEAVAEYLVLAALF
                     QRANDLEKAMRAVQHAQKLKPGNTGALNALNLLNEQKPLPLPGHQREVAPQRRPRSRQ
                     LETSKAPSAAQGLDPVTEARQKALSELAGVLFDIPDEEQGTSRRGFQTIVRGTGKLRS
                     RPVDYNRIVLYVGQVVEMQTQGDFAQAALELERAIDAGLDIPAAYYDLGFLQFHNQNL
                     ESATRNLQLALQHPDFVLAARLLLGQIYQRKGELREAAVEYLEALRQADTAIVPPDQA
                     DVLEQLYEPMVETQSQETNEQVQQQVCENISELLIRANWRDHLRQARQQLPVQTDGGP
                     PVPLAEIVVQARSSHIVESLTKIYQLERSGYLRSAIEEALFALEYAPTYLHLHVTIGE
                     LLLKLGEVEEAIEKFQVVAQAYSSRGEPRQALRIYRRVIELAPIELVPRERLIELLTS
                     MGRIEEALEEYMGFAEVHYSLADLEKARKTYVDAMRLAQKSQADRSMQIRILHRLADI
                     DLQSLDWREALRGFEQVRTLDPSDEEARSRIVEINFRLGQESRALAELDNYLEYLDGR
                     GKNDLALKFLEAQAKEEPQRVSLIRRLAERYHQAGQTSEAIAKLDEAGEILMQIGDVK
                     EAIQVVERILALRPPNAEEYQKLLRQLKSES"
     gene            complement(10228..>12168)
                     /gene="polA"
                     /locus_tag="AC16CB_08120"
     CDS             complement(10228..>12168)
                     /gene="polA"
                     /locus_tag="AC16CB_08120"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIR3342473.1"
                     /translation="MQSYGMNAVQKGQQLSLFAEAAPAPQDKAILGEQAGLQTRIVDT
                     LEDLEKLVKRLNNAPVISFDTETTSTDQMSAQLVGISLAVDGEAGYYIPVGHRAGKQL
                     PLDGVLEALEEPLTNERIPKAGHNLKYDYVVLARYGLRVRPLAFDSMIAEWLINPASR
                     NLGLKNLAWVRLDYRMTEIEELIGKGAKQISMAEVPIEQAGAYAAADAAVVLRLMPQL
                     QAEMEARQANKLLNEVEMPLVEVLANMEMAGIALDTDFLAQMSAELSERMSAIEVQIF
                     QQVGVSFNLNSPQQLSEVLFDRLKIQPPDRTQKTSSGFYSTAAGVLESLSGKHPVVDW
                     VLEYRELSKLKSTYLDALPQQVNPRTGRVHTSYNQTGSVTGRIASSDPNLQNIPIRTD
                     LGRQVRQAFVAGAGMRLVSVDYSQVELRIVAHIAGDEAMLGAFRSQQDIHVATAAAIY
                     NVPIEAVDREQRRHAKAINFGLIYGMSAFGLTRTTDLTLAEAEDFVAAYFKQFPGVKR
                     YLDGVRRQAAEQEYVETLLGRRRYFPGLRNQSNRNIRNREEREAINAPIQGTAADIMK
                     IAMLGVPPALAEAGLSGRMLLQVHDELVLECPEEEVLLVAAVVQKVMESAYRLSIPLA
                     TEARQGVNWGVMSPVRNSNERK"
BASE COUNT         2969 a         3466 c         2929 g         2804 t
ORIGIN      
        1 gcctagtata cacggaacgt ttgaattgga aaacccctaa ctaatccctt ggcattcgac
       61 cttgtgaaac cactatagat cgttcactaa ttcggcatta aagattcata gctcacccag
      121 tgttataatc tcaacaccag attttgacca aaacatcctc ttgttgaata ttgccaagac
      181 atataaggag gttatataca tggttctccc catcaatgcg tatttcaaag gaaagatcgt
      241 tccatactca gaggcgaaaa ttggggtgct gactcacgcc ctcaattacg gcacaggtgt
      301 atttggtggt atacgcggtt actggaattc ggaagaagag caactcttta tatttagacc
      361 cactgatcat tacaggcgat ttctcaactc ggcaaaaatg atgtgtatgg aaatcaacca
      421 tacagtcgaa agcctaaccc agatcactat cgatttgcta cgtgttgatg gataccagcg
      481 agatatctat atccggcctc tggcgtttaa atcggacgag atcatcgggg ttaaactgca
      541 tgacctgcat gatgaactct caattgttgc cgtgcctttt gaacgctacg taagcaacga
      601 cacaaatgcg catgtgacca tctcttcctg gcggcgggta gatgataatg ctatcccagc
      661 gcgtggaaaa atcagcggcg cttatgccaa ctccgcgctg gtcaaaaccg atgcagtgcg
      721 cgccggcttt gacgaagcac ttgtactgac ccaagaaggc cacgtttctg aagggagcgc
      781 catgaacata ttcgtgatcc gagatggcgt tgtctttacc cctccaatga ccgataacat
      841 cttggagggc atcacgcggc gcacagcaat cgaacttttg aaggaggaat tcggtctgcc
      901 ggtggtggaa cgccccatcg accggacaga agtctacctt tgtgacgagc tattcatgac
      961 cggtaccgcg gctcaaatca caaccgtggt taaagtagat aatcggccat tgggctccgg
     1021 tgtcatggga ccgatcacca ccaagctgcg gaaagccttt gacgacgcaa tacgcgggcg
     1081 gaacccccgc ttccgccatt ggaacgtgcc tgtttatccc aagccgtcta ctcaagaaca
     1141 aaaatcaaca gtcagcgaga agctggctta ttgaggaaaa ggacacgccc caggctcatc
     1201 tggggcgtgt ccttttcatc caacttacag cgcgggtcgg cgttgctgcc attgcgttgc
     1261 aagctcataa gccgttccag cgctcagcac agatgcttcg gcccatggac gggagatgat
     1321 ctgcaatcca accggtaagc cctcggatga ataaccgcat ggtaaagata gggcaggcaa
     1381 gccggtgagg ttgaatggcg ctgtgaaacg ggttaataat cgcgcttgct caattgcatt
     1441 aggcccctcg attggaggcg ccgtcacggg agtggtcgcc gtgagcagga tatcgtagcc
     1501 ctcgaagaag cgctcgtaga cacgccgcaa tcgactttgc tcgcggcgca aacgggcata
     1561 ttcagaagat gtgcgcgctg ctccaccttg catgcgctcg aggacatccg ctccaaaatc
     1621 ctcggagcgg gtttgaatac gctctatatg aaatgctgcc gcatcgctgg tcacaatccc
     1681 cccatttgcc gccgcggctg cgtacccctc tggaaactcc accggcacaa cttgagcgcc
     1741 aagttctttg aatacctgcg cagcctcttg tagcgctgcg ttcacctcat catcaacacg
     1801 gctgaaaaat tcatctactg ccagagcgat cttccagcct cgaactccat tctctagctg
     1861 cgctaaataa tcctgcgtgg ggacatcgat agaggctgga tcatccgggt catagccggc
     1921 agtagctgcc aggaggattg cagcatcgcg cacgctgcgc gccattggac cagcatggtc
     1981 caagttccag ctgagcggga tgacaccctg caggctaatc ctgccaaagg tcggcttgag
     2041 ccccacaatt ccgcacagag aggctgggat gcggatcgat ccacccgtat cgctgccaaa
     2101 agatcccatg cacatcccag cggctaatgc cgccgcagag ccacccgatg agccaccggt
     2161 gactcgctcc aaactccacg ggttccggca cggtccaaag tgagggttta cattggtcac
     2221 tccgagggcg atctcgtgca tattggtctt gcccaacatg acaaccccgg ccgcttttag
     2281 ctttttgacg acgacaccat ccgactcagg gatataatcc gcgtagacct tcgacccatg
     2341 ggttgtccgc acaccagccg tctcgtacag gtctttcagc gccagcggga tgccgtgaag
     2401 cagtccgcga tagttaccct tttggatttc gccttctgct tggcgggctg ctgccaatgc
     2461 cgtctcctca gtgacggtga tatagctatt tatgagcggc tcaaattggc ggatacggtc
     2521 aaaatatgcc tttgcaagct cgaccgggga gagctcccgc cgcgagatca ggtcggcagc
     2581 ctggctgatg gtaaggaaag tgagatccat gatatcctct ttaggtattt aacatgcgct
     2641 ccaattccgc ccgcgcttga tccggcgagc gaaaatggat agcgcgcatc ccaagccagc
     2701 gcgctgcgtc cacgttttcc acaaaatcat ctacgaaaac agcttcatcc gcccccacac
     2761 ccaagcggct caatgtaagc tggtagatgc gatggtcagg tttagccagc ccaatctctg
     2821 ctgaaattat tacctcatca aagtcgctgg cgatcttcca ttcattttcc agtatatccc
     2881 gcatattatc ccaggcattg ctcaacaagg caaccttgta gcgctcacgc agagtgccca
     2941 gatatctgag cagagattga tccaagaggt cgccgtccca aaattgctgg atagccttgg
     3001 gaaactcatc gggggatatg ccaaagtgag agcgaacctg ttcccagtgc tcttgggtgc
     3061 taatttttcc caacgtcgcc aggcgggcgc tctcactaga aaatatttgg tcataaagct
     3121 cattgagact cacccccaga cgagcggcaa gcatgcggcg cggttcttga tcgacagtgc
     3181 gcatgagcac accacccatg tcgaagatca ccgctcgcaa gttcacaggc gactgggcat
     3241 cctttgttat atcattcatc cttgtccatt tctctcctgc tgatcattgt cggtcatgcg
     3301 aaacttcagc cacacgggga caaccgccag caaggaagcc caggcggaga gtgcgaaggg
     3361 ggtgcggggg ctaaatctct cccaaagctg tgctccaatc accggggctg gcaacgaaac
     3421 cactcccagg ctggtactga ataggccaaa ggctgtaccg cgtaatctct ccggcactgc
     3481 cttactcatc aaagaaaaga aggctggcgt catgaaaccc atccccaatc ctaaaagcgc
     3541 ccaggctgca ccaaaacccc aaaagctgct gacgcggatg aaaaccatca aggcagcaaa
     3601 ctggaaacca aatccgagag cgatactaac ccgttcgccc tttttatccg ccagccagcc
     3661 ggcaggcatt gccaccagca tcatcgccat accaaatatc gactgcagcc aacctatttg
     3721 ctggacgcta atttggccga tatcctgcat gtagatcggt aacaagttga acgacagggc
     3781 atacgcccaa tcacgcaccc catcggccag caacaaccag gtaaccaggc ctcctcccag
     3841 caacagcgcc agcattgtac ccatgttggc gcgcaggttt atcaaagaaa gcggctgcgg
     3901 gctggtatct ttggtaaaag cagcgttacg cgccatactc atgcggatca cggtcgccag
     3961 tgtatagatt atcccagacc aaactagcat aaacttgaag ccatagagat ccaccagcca
     4021 accacccaac ggcggtccga tcactgcgac caccataaag atggtctccg taataccgaa
     4081 gacgcgggcg cgtgtctcct cggtcgagtt ctctgcaatg aatgcgccaa aactgggcgc
     4141 caccagtgaa cgggtgatcg caacaaaccc ctcacctagc agcacccact gccatgtggg
     4201 ggctaggatt accaccaggt aggagaacac acctgccagg ctgcccaaag caatactgcg
     4261 caagcgtccg agcgagtccg aaatccagcc tcccagaatt agcagcacca ggggtatgat
     4321 ctgcgccagt gtaaagaaca tacccacctg caacacatta gcattcaagc tcttcaaata
     4381 cagcggcaat aacggatcat acatgtggcc tgcgatattc gccagaacca ttgccaccat
     4441 gaaaagccgc agggcattat ttaggagagg cgctcgggtc tgcgtaccca gggatgtacc
     4501 tcctcttgta tccatttcta ttttagccga cattaatcat tctctcctaa aaatgatcga
     4561 tcttcacagg gcaattgcat atgccctgag gggcatgcac aatcccgcaa aaagagtgtt
     4621 tggggtgttt tggtgggggc atacccccac caaaacaccc cattttctct cctcagtggt
     4681 gggaaatcgc cctaatgcga ttgccctatc gatcttcatt agccattccg gtgtaatttt
     4741 ccagtccttt tggcgtagcg ctcagccaga gaaaaaacct tcagtccggc gggaatcgcc
     4801 aacaagccca tcaacagcgt cggccaaatg taaggccata gctgcgaagt aggtaatccc
     4861 tccagtagag cgcgacgtgt gccgtccagc acataggttg ccgggctaaa aactgccaac
     4921 ctttgtagcg cgagcggcaa gacttcaatc gggtagtaga cgccagatac cagaagcagc
     4981 aaagcgataa tgacgtgggt catctgggcg ccccgctcgg ggaagagcag aggcaagata
     5041 gaacccatca cacccacccc aatgaaagac aggctgcctg ccagcagcat caatgttcca
     5101 ccaagcaggt tggcatcgga caggtcaagc tggaacaata ccgcggtcac caccagtatt
     5161 accgcagtga acagcaggct atagatgaca gcaaagaatg tctgtccagc catgtgggtg
     5221 atgcgccgga taggggccat caacgtgtat tcgatggtcc cttcccagcg ctcaatgctg
     5281 atcacctcag taatccagta gaagatcaat gagagatagc gccataccag cgtaccaatc
     5341 accaggtaaa gaaccaggta tcgcccatct acattttgcc caccgccaat tcgctccata
     5401 cccagaccga tgaagctaac tgataagctg ttggctattg agtagatcat ccagaccagc
     5461 tcccaagacc agtagcgttt gaccaggttg gcattgcgct ctataaaggc gtatgttgct
     5521 cggaattcgt gcaccaatgt tgtcatcgat tgtctcctaa catctaactc gatcacacaa
     5581 agcgtaataa gttgccattt ttagaagcat ctatatttcg cattaattct tcccattcat
     5641 cccgtaaaat gcccatgtgg atgatatccc tgcgtaagcc ttctcgataa acaaccttgc
     5701 gggcgcgccc ttcgggttga aatccagcct tctcataggc acgcatggcg cggggattgt
     5761 actcaaagac gcctaagctc acgcgatgta agttcaactc cctgaaagca tagcggagta
     5821 taagtcgcat agcatctgta ccataaccct tgccccaata ctcgcgctca cccaagccaa
     5881 ttcccaccca cgagttgcca tggctccatt gagtaacaaa taacccaata aatccgatga
     5941 gcttatcttc ttcgagagtg cgtatggtga aaaagaaatg cgagtgctga ccttcctcga
     6001 gatctttttc cagccattcc ttaatgcgct caggtgattt caagcgggca ggctcgtcgt
     6061 ccagcagacg agcgtactcc gaatcccgat gccagcggga caaagcctct gcaatcgtct
     6121 gaaggttatc actgccaaga cggaccagtt cgccgtgaaa gatgtcttcc atttctctta
     6181 ctccattcgt ctttcaaaat tccaaaatat attaaatccc atcgccgacc ttccctgtac
     6241 acagcctggc gctgacgcgt ttcctctaca aatccagcat tttggaacag gcgtgtggct
     6301 gccaggttat attcaggaat gtcggctgcc aggctatgca gattcagctc tgcaaatgcg
     6361 tagcgtaata acaagctcaa agcctgagtc ccataaccct tgcctcgctg agttgcatcg
     6421 ccgatgccta actgaaccaa gccgaagccg ctcgtccacc caatccaata gatacgggca
     6481 aacccgatca gctggtcatc tgaacgaagc cggatggtaa agtaaaacag gttctttttc
     6541 tcatcggctt ctttttcaat ctctgtgtaa cgtttcttaa cctgttcggg ggacaaggga
     6601 cgcgcaggct caagattcag ctggcgcatg tactccgcat tctgggtcca tatagcctcg
     6661 atctccgcgt ccttatccgg atcgataggg gcaagacata tctgctcgcc cgtgaatagt
     6721 gatgtcttga tatccattgt tcatactcca atgacctgaa aatgattaat cactttctct
     6781 aactaatttc ttgccggtaa gctcgaggaa cacatcctcc aacgaaggct cgtgtccatt
     6841 tcgagggaag gtgcgcttca aagcttcagg tgaatctagc gcgacaacct tcccgccatc
     6901 aatgatggcg atacgctcac acaagttttc ggcttcgagc atgtcgtgcg tagtcagcaa
     6961 gatggtcgta ccatattcct gattgagctc gcgtacaact gcctgcactt cccgctttga
     7021 gcgaggatcc aacccagtgg tcggctcgtc gagcagcaac aggcgcggcc gggatagtaa
     7081 ggctcgggca attgcaacct tttgctgcat accgcgcgac atctcttcca tcggcttgta
     7141 gatcgtccgc tcttccagcc ccaagcgtga caggatttct acaacacgct gtcgagtctc
     7201 acgcccttcc atgccgtata accgagcacc ataaagcagg ttttccatcg gcgagagctt
     7261 cttgaagaag ctagcttcta ccgatacacg gttgatcaac cgctgaactt ctattggttg
     7321 gcgaacgaca tcatgcccaa agacaacgat gcgaccggcg tctggcaaaa gcaatgtgga
     7381 aatcaggcgt atcagggtag attttccaga accatttggt ccaagaacgc caaaaacttc
     7441 gccttccttc acataaaaag agacatggtc caccgccacg acaatcgact tcgagccatt
     7501 tttcgaatcg ttttcatttg atatcgaccc tccaacccca ccgttcgagc ctctttgatt
     7561 ccacagccag cgcttccaaa ggggttggga ggacttcccg aagtgtttgt aggtatcttg
     7621 taaatctatt gcgtacattg ctctgacctc cacagaaaaa aattggccac gggcgtaccc
     7681 gtggccagcg aaagccaaga aaaaagccac ggggctggag agcacccgtg gccagttact
     7741 caagataaga ttgaccgaat gatcaacagg cgcactccaa gaaggcacaa ccaaccgcat
     7801 cgccgatagg tgaagcgttg cgcaggacaa aaatctttgt gcatatgtct ttcatgaaat
     7861 gcgctactcc ttagattaaa gctttattag aacgaaccga gtttaccaca ctagtcaaag
     7921 tcgtgtcaag actcgctttt caattgtcga agtaatttct ggtattcctc tgcattcggc
     7981 gggcgtagcg ccagtattct ctcaacaacc tggatagctt cttttacatc gccaatctgc
     8041 atcaatatct cgcctgcctc atccagcttg gcgatcgctt cgcttgtttg ccctgcctga
     8101 tgataacgct cggcgaggcg tcggatcaga cttactcgct ggggttcttc tttagcttgc
     8161 gcttccaaga actttagcgc caaatcgttc tttcccctac cgtccagata ctccaggtag
     8221 ttatccagtt cagccaaggc acgcgattct tgcccaaggc gaaaattaat ctccacgatg
     8281 cgggagcgag cttcctcatc agagggatcc agtgtgcgaa cctgctcaaa gccgcgcaat
     8341 gcctccctcc agtctaggct ttgcaggtcg atatcagcta ggcgatgcag gatacgtatc
     8401 tgcatggagc ggtccgcctg ggacttttgt gcaagtcgca tggcatcaac atacgttttt
     8461 cgcgcttttt ccagatctgc caagctgtaa tgtacttcgg caaatcccat gtattcttct
     8521 aaggcctctt cgatccgtcc catggaagtg agcaactcga tcaaccgctc tcgtggaaca
     8581 agctctatgg gagctaactc tatcacccgc cggtatatcc gcagcgcctg gcgtggttca
     8641 ccccgcgaac tatatgcctg cgctaccacc tggaacttct caatggcttc ttctacctcc
     8701 cccagcttca gtaacagctc accaatggta acgtgcaggt gaagatatgt gggtgcatac
     8761 tccaaggcaa acaaggcttc ctcaatcgcg gagcgtaaat atccagaacg ttccaattgg
     8821 taaatcttgg tcaacgattc aacgatatgg ctgcttcttg cttggaccac tatctcagcc
     8881 agcggaactg gcgggcctcc atcagtttgc accggaagct gctggcgcgc ctggcgaagg
     8941 tgatccctcc agtttgcccg tatcagcaat tcggagatat tttcgcagac ttgttgttga
     9001 acctgctcat tggtttcttg gctctgtgtt tcaaccatcg gctcgtagag ctgctccaaa
     9061 acgtcagcct ggtctggcgg gacaattgcc gtgtcggcct ggcggagcgc ttcaagatac
     9121 tccacagccg cctcgcgtaa ttcacctttt ctctgataaa tctgccctaa aagcaaacgc
     9181 gctgccaaca caaaatcagg atgttgtaaa gcaagttgaa ggttgcgcgt agcactttcc
     9241 agattttgat tatggaattg cagaaagcct aggtcataat atgctgcggg gatatctagt
     9301 ccagcatcga tagctcgttc cagttccaag gcagcctgtg caaaatcacc ttgtgtctgc
     9361 atctctacca cctgtcccac gtagagcaca atacgattgt agtctacagg tctactgcgc
     9421 agcttacctg tcccgcgcac aatcgtctgg aaaccgcggc gggaggtgcc ttgctcctca
     9481 tcggggatat cgaaaagaac acctgccagc tctgataagg ctttttgccg cgcttcggtt
     9541 acaggatcga gtccttgagc agcggacggt gccttggaag tttcaagctg gcgcgagcgt
     9601 ggccgccgct gtggcgcaac ttctcgctgg tgacccggta atggcagtgg cttttgttca
     9661 ttcaacaaat ttaacgcgtt tagcgccccg gtatttcccg gcttgagctt ctgggcatgt
     9721 tgtacagctc gcatggcttt ttctaaatca ttggcgcgct gaaacaacgc tgccaacaca
     9781 agatactccg ccactgcctc atttgcttta cccaggcgct catagaccag tgccaatcgt
     9841 gaacgcgctg taaagttgtc gggctggaaa ctcgtgacac gcccccagtt ctctatggct
     9901 ttatcgacat cccgcttctt caggtgcaat tctgcggcct ggagagaagc agcgcgggcg
     9961 cgctccaaat tgcccatttg ctcataggat tgagatattt tctctaacgg tacggggtca
    10021 tccggtgaga ccatagcagc cttggcataa gctcgcaaag caccctcgta atcttcaagc
    10081 tcaaataaag ccaggccaag actggtaagg gcttggtgat gctcagggaa ctgctctaaa
    10141 gcctgccgat aaaatcccgc cgcccgttcc caggattgat cccacgccgc ggaatggcct
    10201 tcgtccatag ctttttggaa gatgtcttta tttccgctca ttcgaattcc tcaccgggct
    10261 cataacgccc caattcactc cctggcgggc ttcggttgcc agggggatac tcaaacgata
    10321 agcactttcc atgactttct gcacaactgc tgcaaccagc aatacttcct cctcagggca
    10381 ctccagcacc agttcatcgt gtacctgtag cagcatgcga cctgaaaggc cagcctccgc
    10441 aagcgccgga ggcacgccca acatggcaat cttcatgatg tcggcggcgg tgccttggat
    10501 aggggcatta atcgcttccc gttcttctcg gttgcgaata ttgcgattgc tctgatttct
    10561 caatcccggg aagtagcggc ggcggcccag caaggtttcc acatattctt gctcggcagc
    10621 ctgacggcga acgccgtcca gatagcgttt caccccgggg aattgcttga aataagcagc
    10681 gacaaaatcc tctgcctcgg ctaaggtcag gtcagtggta cgcgttaaac caaaggcgct
    10741 cattccataa attaacccaa agttgatggc tttggcatgg cggcgctgct cacggtcgac
    10801 tgcttcaatg ggaacattgt atatagccgc cgctgtagcc acatgaatat cctgctgtga
    10861 ccggaaagct cccagcatgg cctcatcgcc agcaatgtgc gccacgatac gcaactccac
    10921 ttgcgaataa tcgacagaca ccaggcgcat tcccgcacca gccacaaaag cctggcgcac
    10981 ctgccgcccc aggtctgtgc ggatgggaat gttctgtaaa ttaggatccg atgatgcgat
    11041 gcgtccagtc acagatccgg tctgattata ggaggtatgc acgcgacccg tacgcgggtt
    11101 gacctgctga ggcagggcat ccagataggt ggatttcagc ttggaaagct ctctgtattc
    11161 gagcacccaa tccacgaccg ggtgctttcc actcaaagac tcgagcacgc ccgccgcagt
    11221 agaataaaac ccgctggagg tcttctgagt gcggtcgggg ggctggattt tgaggcgatc
    11281 aaagagcacc tcagagagct gctgcggcga attcagatta aaggaaacac ccacttgctg
    11341 aaagatctgc acttcaatcg cgctcatgcg ctcactcaat tcggctgaca tctgtgccag
    11401 aaaatctgta tccagggcaa tgccagccat ctccatattg gctaacactt ccaccaaggg
    11461 catttcgact tcattcaaaa gcttgttcgc ttggcgcgct tccatctcag cctggagttg
    11521 cggcatgagg cgcagcacca ccgctgcgtc agccgcggca tacgcacccg cctgctcgat
    11581 tggcacctcc gccatgctga tttgcttggc acctttgcca attagctctt caatttcggt
    11641 catgcgataa tccaggcgca cccaagctaa attcttcagt cccaggttac gagaggctgg
    11701 attgatcagc cactcagcga tcatactatc aaacgcgagt ggtcgcacac gcagaccata
    11761 acgcgccagc acgacataat cgtatttcaa attgtgtccg gctttgggaa tacgctcatt
    11821 ggttagaggc tcttctaaag cttccagcac cccatctagc ggtaactgct tgcccgcacg
    11881 atgaccaacc gggatgtaat aacctgcttc cccatcgacc gccagggaga tgcccactag
    11941 ctgggcgctc atctgatcag tagatgtcgt ttccgtatca aacgagatta ccggtgcgtt
    12001 attcaaacgc ttcactaact tctcaagatc ttctagggta tccacaatcc tggtttgcag
    12061 gcctgcttgc tctcccagaa tagctttgtc ctgtggagct ggtgctgcct cagcgaacag
    12121 ggatagctgc tgaccttttt gcactgcgtt catgccgtag gattgcat
//
LOCUS       DBNGSX010000266         3331 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1683786, whole genome shotgun sequence.
ACCESSION   DBNGSX010000266 DBNGSX010000000
VERSION     DBNGSX010000266.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1683786"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2879)
                     /locus_tag="AC16CB_08125"
     CDS             complement(<1..2879)
                     /locus_tag="AC16CB_08125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase activity-associated protein Agl23"
                     /protein_id="HIR3342474.1"
                     /translation="METTLEKRTSWLDRPVVSAITLNWETILFATILIVAVVSRFYDL
                     GARVMSHDETSHVYFSWLYEQGNSYSHDPVAHGPLQFHLVAFSYFLFGDSDFSARIPV
                     AIFSIATVFFMWYFRRFLGRAGAIVAALLLLISPYILYYGRYVRNEAFVGLFGVITLW
                     AILRYLESGENRYLYWLTIATVFHFTAKETSFIYTAQALIFLAIYLVYRLIQNPWPEP
                     GWRKYFIFAIIVALLLITITGGTLWVTRSSTTLDASETASPAVPGEDLAASTPTGLAA
                     LPSIFIISTLLALLVVAYTALRGYSWSLLRNERSFGMLILLGSLVLPMLSPFPVELLG
                     HNPIDYQNTQTIIWDAIFIAIFTLIAVVIGLLWNPRLWLVNAALFYAIFTVFYTSIFT
                     NGFGFVTGLVGSLGYWLEQQGVNRGSQPWYYYGLIQLPIYEFLPALGTLGALFYALWR
                     GQLSATEPESSPQTSTVDTQDTPLDDQDTLGTDQTATSLPVPVSESPPTLSLLLFWSF
                     TSLLAYTIAGEKMPWLTVHITLPMILTAGWYLGFLIDKTDWRLFLRQRGILVVLVLPI
                     FFISLLATIGQVLGTDPPFQGTQLNQLQATSTFITALLTTLGSGWALTLWVKPWASSQ
                     LQRVVILTGFALLGLLTARAAIRAAYINYDNAKEYLVYAHMARGPKEALEQLEEISQR
                     TTNGLALRVAYDNETTYPYWWYLRNFTNKDYFGTTPTRAQRDAVAILVGDANYGKIEP
                     VVGQAYHEFEYTRIWWPNQDYFGLTWERVRDAFTDPKMRSALYKVWLNRDFTEYSQLT
                     VQDMSLPNWQPSNKFRLYVRKDVVASLWNYGAAPAEEIVLADPYEGKEIKLSADRAVG
                     TLGTEPGQFKRPRGIAVGPDDSLYVADTENHRIQRFAPDGTFINAWGSFAASTEGAPA
                     AEGMFNEPWGIAVGPDGAVYVADTWNHRIQKFSAEGEFIKTWGFG"
     gene            complement(2940..>3331)
                     /locus_tag="AC16CB_08130"
     CDS             complement(2940..>3331)
                     /locus_tag="AC16CB_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342475.1"
                     /translation="NSKTARHWLPSIIRITSFQVQFVTWVRATLVLLSVLFFGLGIPN
                     PSTMIETSKPLVTYNSAMASVDAAPLTQNYQIQAHHPVPIPTPASSSLPDELPKVQNT
                     GMRFTKNKPAIAHTPKPSPISLHDMER"
BASE COUNT          914 a          748 c          884 g          785 t
ORIGIN      
        1 ataccaaacc cccaagtctt gatgaattcg ccttctgcgc tgaatttttg aatcctgtga
       61 ttccaggtgt cagcaacata taccgctcca tctggaccaa cggcaattcc ccagggctcg
      121 ttaaacatgc cctccgccgc aggtgcgcct tctgtgctag ctgcaaaact cccccaggca
      181 tttatgaagg ttccgtcagg tgcaaagcgt tggatgcggt gattttcagt atctgccaca
      241 tacaggctat catctggacc aacagcaatg ccacgtggtc gtttaaactg tcctggttca
      301 gtccccagtg tgccgaccgc tcgatctgca cttagcttta tctctttgcc ttcataagga
      361 tcagcaagca cgatttcttc agctggggca gctccatagt tccataaact ggcgacgaca
      421 tccttgcgca cataaagcct gaatttgtta gagggttgcc aattaggcaa gctcatatct
      481 tggacagtca attggctata ctcggtaaaa tcgcggttta accacacttt gtaaagtgca
      541 gaacgcatct tcggatctgt aaatgcatca cgcacgcgtt cccaggtgag gccaaagtag
      601 tcttgattag gccaccaaat acgtgtgtac tcaaactcgt ggtaagcctg cccaaccact
      661 ggttcgattt ttccgtaatt ggcgtcgcct actaatatgg caacagcatc gcgctgggcg
      721 cgcgtgggtg tagtcccaaa ataatctttg ttggtaaagt tgcgcaggta ccaccaatag
      781 ggataggtgg tttcattgtc ataagccacg cgcaatgcca gtccattcgt ggtgcgctgg
      841 gagatttctt ccagttgctc cagggcttct ttaggaccgc gggccatatg agcgtataca
      901 agatattcct tggcgttatc gtaatttata tatgcggcgc ggatggctgc gcgagccgtt
      961 agcaagccta atagggcgaa accggtcagg ataacgacgc gctggagctg tgatgaggcc
     1021 caaggtttta cccatagcgt aagtgcccac ccacttccaa gggttgttaa taaagcggtt
     1081 atgaaagtgc ttgtcgcttg taactgattg agttgtgtgc cttgaaatgg cggatcggtg
     1141 cccagtactt gcccaatcgt agcaagcaag ctaatgaaaa atatgggcaa caccagcaca
     1201 accaagatgc ctcgttggcg caagaagaga cgccaatcag tcttatcaat caaaaagccc
     1261 aagtaccagc cagcggtgag aatcatgggt aaggtgatat gcacggtcaa ccagggcatt
     1321 ttttcaccag cgatagtgta ggctaatagg ctggtaaagc tccaaaatag aagcagcgag
     1381 agcgttggtg gagattcgct cactggcaca ggcagggaag ttgctgtttg atctgtgccg
     1441 agggtatcct ggtcatcgag cggagtgtcc tgtgtgtcca ctgtggaggt ttgaggggag
     1501 ctttctggtt cagtggcgga tagctgaccc cgccataggg cataaaagag ggcgcccaat
     1561 gttcccaggg cgggtaggaa ttcatagatg ggcaattgaa ttagcccata gtaataccag
     1621 ggttggctac cgcgattaac accttgctgc tctagccaat aaccaaggga gcctaccagt
     1681 ccggtgacaa atccaaaacc gttggtgaat atactggtat aaaaaactgt gaagattgca
     1741 taaaacaagg cggcgttaac aagccacaag cgcggattcc acagcagccc aatgacgaca
     1801 gcaatcaaag taaatatggc tatgaaaatg gcatcccaga taatcgtttg ggtattctgg
     1861 taatcgatgg gattatgacc aagaagttcg acagggaagg gggagagcat ggggagcacc
     1921 aagctgccaa gtagaatcag cattccaaag gagcgttcat tacgcaataa gctccatgaa
     1981 tacccgcgga gggcggtata tgcaacgaca agcaaagcaa gaagggtcga gatgatgaaa
     2041 atggacggca atgcagccaa tccagtgggg gtgctggcag ctaggtcttc accaggcacg
     2101 gcaggtgaag ctgtttcaga tgcgtctaaa gtggtggagc tacgggtcac ccaaagggta
     2161 ccgcctgtta tcgtgatgag cagaagcgca acgatgatgg caaagataaa atatttgcgc
     2221 cagcccggtt ctggccaggg attttggatc aaacggtaaa caaggtatat tgctaaaaat
     2281 atgagcgctt gagcggtata gataaaagaa gtttctttgg ctgtgaagtg aaatacggta
     2341 gcaatagtaa gccaatagag gtaacgattt tcgccagatt ctagataacg caagatcgcc
     2401 cacagtgtaa tcacgccaaa taagccgaca aaggcctcat tgcgcacata acgcccatag
     2461 taaagtatgt aaggggatat taacagcaac agcgcagcga cgatcgcacc ggcgcgcccc
     2521 agaaaacggc ggaaatacca catgaaaaat accgtggcga tactgaagat ggctactggg
     2581 atgcgggctg agaaatcgct atctccaaag aggaaatatg aaaaagccac caggtggaat
     2641 tggagcggtc catgtgctac cgggtcatga gagtagctat tgccctgttc gtatagccac
     2701 gaaaaatata cgtgcgaagt ttcgtcgtgg ctcattacgc gcgcaccaag gtcgtaaaag
     2761 cggctaacca ctgccacaat caggatagtc gcaaataata tagtctccca gttcaatgtg
     2821 atagcagaaa ctacaggtcg gtcgagccac gaagtgcgtt tttcaagggt ggtttccata
     2881 ctgatcctta gaagtcttat ttcccagggg ttaattcaag attatcatct catatgggct
     2941 taacgctcca tatcatgtaa cgaaataggg ctgggtttgg gtgtgtgggc tatcgcaggc
     3001 ttgttttttg taaacctcat accagtattt tgaacttttg gcagttcgtc aggcaacgat
     3061 gacgaagcag gcgtaggaat gggaacaggg tgatgtgcct ggatttggta gttctgggtg
     3121 agtggagcgg cgtcgacgga tgccatcgcc gagttatatg tcaccagtgg tttggaggtt
     3181 tcaatcattg ttgatgggtt tggtattccc aggccaaaaa acagtactga cagcaatacc
     3241 aaagtggctc taacccaggt aacaaattgc acctggaaag aagtaattct aattatcgaa
     3301 gggagccaat gtctcgcagt tttagaattg c
//
LOCUS       DBNGSX010000267         2492 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1822240, whole genome shotgun sequence.
ACCESSION   DBNGSX010000267 DBNGSX010000000
VERSION     DBNGSX010000267.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1822240"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="AC16CB_08135"
     CDS             <1..548
                     /locus_tag="AC16CB_08135"
                     /inference="COORDINATES: protein motif:HMM:NF014171.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3342476.1"
                     /translation="GVITVQDIEAAVEQDEHNIDLPVTRFCSQRLVVAYPDETVNQAL
                     ARMSSRDIGRLPVVDPAQTRRLLGWISRSDIIRAYERALTRRVAIQHQLTHARLGAVS
                     GVNVFEFVVASGSAVDGKSLSEIAWPEDSLVASVHRGRRLIIPHGNTVLQVGDRLSIV
                     AHVHDEKLLQALVSAASSSNN"
     gene            656..1429
                     /locus_tag="AC16CB_08140"
     CDS             656..1429
                     /locus_tag="AC16CB_08140"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR3342477.1"
                     /translation="MTPSLTDLEFLARQAGDILRAKYGQRQQVDHKGIIDLVTEADRQ
                     SEAFLLAQIRRRFPDHRILAEESGGQAGDDCCLWYIDPLDGTVNYAHGVPIFSVSLAF
                     QEMGRMRFGVVYDPMRDECFSAEAGSGAWLNGEPIRVSDTPDLDNSLLVTGFPYDIRE
                     NPDNNLDHYTRFSLLSQAVRRLGSAALDLSYVACGRFDGFWELRLSPWDVAAGALIAQ
                     EAGAVVTNIHGGTDYLSPQQSILAANPHIHAVMLAQFEK"
     gene            complement(1437..2237)
                     /gene="surE"
                     /locus_tag="AC16CB_08145"
     CDS             complement(1437..2237)
                     /gene="surE"
                     /locus_tag="AC16CB_08145"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIR3342478.1"
                     /translation="MEKPQILLTNDDGIRSPGLWAAAKALSEIGFVHVAAPREQYSGA
                     GRSMPNHSDGVIQVEQVHVNGDAWIVHAVGGTPAQAVQHAMFEILPQRPSLIVSGINY
                     GENLGSGVTISGTVGAALEGAAFGIPSLAISLETSVNHHLSYSDDVDFSIAGHFTALF
                     GRKLLEHRLPPDVHVLKVDVPADATPATPWEITRLSPTNYFTAIPTQRRSWSEPGKIS
                     YRVSDGLQDEPPGTDIYALHIQRHVAVTPLSLDLTSRVDLMILKDSLE"
     gene            complement(2203..>2492)
                     /locus_tag="AC16CB_08150"
     CDS             complement(2203..>2492)
                     /locus_tag="AC16CB_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004938178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HIR3342479.1"
                     /translation="LEAWKKDDAMQIEVLEQSEGRFAFDVTRCRYAEMYNALGVPELG
                     GLLSCNRDHALIDGFNPQVQLRRTQTIMEGAPSCDFRYTIYGKTPDTPNQR"
BASE COUNT          560 a          657 c          705 g          570 t
ORIGIN      
        1 gtggtgtgat cacagtgcag gatatcgaag ctgctgtgga acaagacgag cataacatcg
       61 atctgcccgt gacccgtttc tgttctcagc ggctggtagt agcttacccc gatgaaaccg
      121 tcaaccaagc cctggcacgc atgagcagcc gggatattgg tcgccttcca gtcgtcgacc
      181 ctgctcaaac gcgtagactt ctcgggtgga tcagccgttc ggatatcata cgggcttatg
      241 aacgagcgct gacgcgacgc gtggcgatcc aacaccagct gacccatgct cgtttgggag
      301 ctgtctccgg tgtcaatgtt ttcgagttcg tggtggcatc tggcagtgca gtggatggga
      361 aatcgctgtc ggaaattgca tggcctgaag atagcctggt agccagtgta caccgtggtc
      421 ggcgtttgat tattccacat gggaatacag tactacaggt aggagaccgg ttgtcgattg
      481 tcgctcatgt gcatgatgaa aagttgttac aggcattagt gagtgcagca agcagctcga
      541 ataattaagg taacaaaata ccatgaaggt gctacacacc gacctgtgcc aatggtgaag
      601 aaatcctgcg cgcgctgaaa aatatctatc ctttattagg acagtacaac agcttatgac
      661 cccctcatta acagacctcg aatttttagc acgccaggcg ggtgacatcc tgcgtgccaa
      721 gtacggtcag cggcaacagg tcgaccacaa gggaatcatc gacctggtca ccgaagcgga
      781 tcgccaatcc gaggcttttc tactggcgca aatccgtagg cgctttcctg atcaccgcat
      841 cctggctgag gagagcggag ggcaagctgg agacgactgc tgtctgtggt atatagaccc
      901 gttagatggt acggtcaatt atgcccatgg cgtcccaatt ttctctgtat cactggcatt
      961 ccaggagatg gggaggatgc gcttcggcgt tgtttacgac ccaatgcggg atgagtgctt
     1021 cagtgcggag gcggggagcg gtgcctggct gaatggggaa cccatccgcg tctcagacac
     1081 acctgatttg gataacagcc tgctggtcac cggttttccc tacgatattc gcgaaaatcc
     1141 ggataacaac ctagatcact atacccgctt ttcactgctc agccaggcag ttcgccgcct
     1201 gggttcggct gctctagatt tatcttatgt agcctgcggg cgtttcgatg gcttttggga
     1261 attacgcctg agcccttggg atgtggcggc tggtgcgctg attgcgcagg aggccggagc
     1321 ggtggttacg aacatccatg gagggaccga ttacctctcc ccacaacaat ccatcttagc
     1381 tgccaacccc catattcacg ctgtcatgtt ggcgcagttt gaaaaatagg gatttattac
     1441 tccagggagt ccttcagtat cattaaatcg acgcgcgaag tcagatccag gctgagaggc
     1501 gttaccgcca cgtggcgctg gatatgcagg gcgtaaatgt ccgtccccgg gggttcatcc
     1561 tgcaatccat cggaaacccg gtagctaatc ttgccaggct cgctccagga gcggcgttgt
     1621 gtggggatgg ctgtaaaata attggtcggg gaaagtcgcg tgatctccca tggagttgcg
     1681 ggtgtggcat cggctggcac atcgactttg agaacgtgca catcgggcgg tagccgatgc
     1741 tccagcaact tgcgcccgaa gagggcggta aagtgccccg cgattgaaaa atccacatca
     1801 tctgagtatg atagatggtg gttcacggat gtctcgaggg atatggccag cgaagggatg
     1861 ccgaaagctg cgccctctag agcagcgccc actgtcccgg agatcgtcac gccgctaccg
     1921 aggttctccc cataattgat acctgagaca atcaagctgg gtcgttgagg caagatttca
     1981 aacattgcat gttgcacagc ctgtgctggc gtaccaccca ctgcgtgcac aatccaggca
     2041 tctccattga catgtacttg ctcaacctgg atcaccccat cggaatggtt gggcatgctg
     2101 cgaccagccc cggaatattg ctcacgcggc gcggcgacat gaacgaaacc gatttcagaa
     2161 agcgcctttg ccgctgccca taaaccgggc gagcggatgc catcatcgtt ggttaggagt
     2221 atctggggtt tttccataaa tagtatagcg gaaatcgcat gaaggggcgc cctccatgat
     2281 ggtctgcgta cgcctcagtt ggacctgcgg gttaaagcca tcgatcaagg catgatcgcg
     2341 gttacaggag agcagtcccc ctaactctgg aacgcccagg gcgttgtaca tctcggcata
     2401 gcggcagcgg gttacgtcaa aagcaaatcg tccttcgctt tgctctagca cctcgatctg
     2461 catcgcatcg tccttcttcc aggcctccaa cg
//
LOCUS       DBNGSX010000268         5030 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1614309, whole genome shotgun sequence.
ACCESSION   DBNGSX010000268 DBNGSX010000000
VERSION     DBNGSX010000268.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1614309"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1104
                     /locus_tag="AC16CB_08155"
     CDS             94..1104
                     /locus_tag="AC16CB_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3342480.1"
                     /translation="MGRTVNLTTNNVEPGETLVEVQNLVKFFPIRKGVLQRVVDWVKA
                     VDDVSFGIRKGETLGLVGESGCGKTTLGITMLRLLQPTSGRVEIEGVNIYDLSTEQLK
                     AIRRKMQIIFQDPYASLNPRKPIGESIMAGLNIHGIGTRQERVDIMLDLLRKVGLEEY
                     YARRFPHEFSGGQRQRIGIARALALQPEFVVCDEPISALDVSIQSQVLNLLKDLQDEF
                     HLTYLFIAHNLSVVEFISDRVAVMYLGKIVELAPREALFSNPLHFYTQAIMSAIPIAD
                     PKAQSQRIILEGEVPTPINPPPGCHFHPRCPWAMEECSVEEPPFIEISPEHWVACWHV
                     GP"
     gene            1152..2423
                     /locus_tag="AC16CB_08160"
     CDS             1152..2423
                     /locus_tag="AC16CB_08160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3342481.1"
                     /translation="MKTIPLWTDEFPKPDDIPVSRLVEKADVAVIGSGYTGLNAALVL
                     AKSGGKVAVLEQESIGWGASSRNGSMLTTGLKSPITQIIRRYGIDVARHLWKWSLDAI
                     DHVEKIIIRENIACDFSRPGHAYLAYKPHHYDKIKAYGELLARMFGYEGFEYIDPKDM
                     HQEIGSDAFYGAMTEAYSGLLQPAKYVYGLARAASQAGVLLVDKCRVTGISRRENGFA
                     LQTSLGEMEAKEVLVATGGYTTNLVPRIRQGIFPVGSYIIVTEPLSANLQAELSPKRR
                     AFFDSKRFLNYFCLTPDGRMLFGGRANLSTGLDATRSANILKKQMYRVFPQLQGTPIT
                     HSWTGKLGVSFDQMPHIGRVNGIHYAYGYSGHGVSIASYLGYEVGRLIAGEISGSVYM
                     DIHHPRFILASLDKLYLPLVSAYFRLEDFLS"
     gene            2486..3478
                     /locus_tag="AC16CB_08165"
     CDS             2486..3478
                     /locus_tag="AC16CB_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3342482.1"
                     /translation="MSDNKNKLLLNVSNLKTHFFTEDGVVKAVDDVSFYVNEGEILGL
                     VGESGCGKTVTSLSIIGLLSKPGKIVGGEIQYDGQNLLNLSYEQLSEIRGNRISMIFQ
                     QPQTSLNPVLTVGDQIAEVLEIHQGVSTKDAWGHAVEMLRQVGIPSPEKRAFAYPHEL
                     SGGQAQRVMIAMALILEPKLLIADEPTTALDVTIQAQILDLMRGLNNIMGTAIVLITH
                     DLGVIAGIANRVAVMYAGKIVEHTMVETLFEDPLHPYTKGLINSIPVLGRVKDRLEVI
                     PGVVPNLIDLPRGCAFAPRCEARIEHGLEICEEKMPEMVQIERGHEVRCWLYPD"
     gene            3694..3915
                     /locus_tag="AC16CB_08170"
     CDS             3694..3915
                     /locus_tag="AC16CB_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342483.1"
                     /translation="MDVVELYQRRAPIEIAPEDFRKVGYQVVDQLANFLECLPQLPVT
                     TGETPTQIRKILGENTLPDDGDSLECVLD"
     gene            3928..4173
                     /locus_tag="AC16CB_08175"
     CDS             3928..4173
                     /locus_tag="AC16CB_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HIR3342484.1"
                     /translation="MVNHSLFNGHSRFWGYITSSAAPIGALADMIAAMINPNVGAWIS
                     SPMASEIEAQTVRWIAEMLGYPPDCGGLSIFLRSANL"
     gene            complement(4190..4411)
                     /locus_tag="AC16CB_08180"
     CDS             complement(4190..4411)
                     /locus_tag="AC16CB_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342485.1"
                     /translation="MINIVLRVLALILIGLASAVIGFIAGSNIFIIFGLEFNGRQGYE
                     AGGPIGFILGALIGLIAGFAPLFVRQSKK"
     gene            complement(4475..4738)
                     /locus_tag="AC16CB_08185"
     CDS             complement(4475..4738)
                     /locus_tag="AC16CB_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342486.1"
                     /translation="MTMLMINIALRILAILLIGFVSAVIGLILGAIIGGNLAAIYEIL
                     FGHEFVFNGRVEYEATGQIGFILGAFRGLVGSGYWLFGSQIKQ"
BASE COUNT         1337 a         1116 c         1298 g         1279 t
ORIGIN      
        1 acacatatga tataagtagc tccaaccggg tatcaggaaa aaacggattc gaatgaaaag
       61 tgttgttgct gatcttattg tttgggagac aaaatgggac gaaccgtgaa cctcaccaca
      121 aataacgttg aacctggaga aacccttgtt gaggtgcaga atttggtcaa gtttttcccg
      181 attcgaaagg gggtacttca gagagtcgtt gattgggtga aagccgttga tgatgttagc
      241 ttcggaatcc gcaagggcga gacgctgggt ctggtaggcg agtctggatg tggcaagacc
      301 acactcggca taaccatgct gcgtttgttg cagccgacca gcggtcgggt tgagattgaa
      361 ggcgtgaata tttatgattt gagcaccgaa caattgaaag caatccgccg gaaaatgcaa
      421 atcatattcc aggacccata cgcctctctc aaccctcgga aaccgatagg cgagtcgatt
      481 atggctggcc ttaacattca cggtataggc actcggcaag agcgcgtgga cattatgctc
      541 gaccttcttc gaaaagtggg attggaggag tattatgctc gtcgctttcc ccatgaattc
      601 tctggtggcc aaaggcagag gatcggaatc gctcgcgcgc tggctttaca acccgagttt
      661 gttgtatgtg atgagccgat ttccgcattg gatgtctcaa tccaatcgca ggttttaaac
      721 ctgttgaagg acttacaaga cgaatttcac ttgacctacc tattcatcgc tcacaacctg
      781 tctgtagttg aatttatatc ggatagggtc gctgtgatgt atcttggcaa gattgttgag
      841 ttagcgcctc gagaggcgct tttctcaaat cccttgcatt tctacactca ggcgattatg
      901 tcggcaatac ccattgctga tccaaaagcg cagagccagc ggatcatact agaaggcgag
      961 gtgccgaccc ccattaaccc tccgccaggg tgccattttc acccccgctg cccatgggct
     1021 atggaggaat gttcggtaga ggagcctcct tttattgaga tatcaccgga gcactgggtg
     1081 gcgtgttggc atgtgggacc ctgacattct tgggatatac acataaattt ttggccatta
     1141 taggctgagt gatgaaaacc ataccacttt ggacagatga gttcccaaaa ccggatgata
     1201 ttccagtaag ccgcttagtg gagaaggctg atgtggcggt catcgggtct ggatatactg
     1261 gactgaatgc agccctggtc ttggcaaaat ctggggggaa ggtggccgtg ctggagcaag
     1321 aaagtattgg ctggggagcc agcagccgaa acgggagtat gctcacaaca ggcctgaaat
     1381 ctccgattac gcagatcatt cgacgctatg gcatcgatgt tgctcgtcat ctctggaagt
     1441 ggagcctgga tgcgattgac catgttgaaa agatcatcat ccgagaaaac attgcgtgtg
     1501 atttctcgcg cccgggtcat gcctatctag cctataaacc tcatcactac gacaagatca
     1561 aagcctatgg cgagctgcta gcccggatgt ttggctatga gggctttgaa tatatcgatc
     1621 caaaagatat gcaccaggag attggcagcg atgccttcta cggtgccatg acggaggctt
     1681 attctggctt gttgcagcca gccaagtatg tgtatggatt agcgagagct gcatcccagg
     1741 caggggtatt gttggtagat aagtgtcggg tcacaggtat ttcccgcagg gaaaatggat
     1801 ttgccttgca gacatccctg ggtgaaatgg aggctaaaga agtgctggtg gcgactggcg
     1861 ggtatacgac caacctggtc ccacgcatca ggcaagggat tttcccggtg ggcagttata
     1921 tcatcgtcac tgagcctttg agtgccaatc tgcaagcgga gctcagtccg aagagacgag
     1981 cgtttttcga tagtaaacgc tttttgaatt atttctgctt aacaccggat ggtcgtatgt
     2041 tgtttggagg ccgggcaaat ctctccacgg gtctggatgc gacccggtca gcgaatatcc
     2101 taaagaaaca gatgtatagg gttttccccc aattgcaggg aacccctatc acccacagct
     2161 ggactggtaa attgggagtc tctttcgatc agatgccgca tattggtcgc gtcaatggga
     2221 ttcattacgc ctatggatat agtggacacg gtgtgtcgat tgccagttat ctgggctatg
     2281 aggtgggcag gttgatcgct ggcgagatca gcgggagtgt gtatatggat attcaccacc
     2341 cgcgctttat cctcgcctcg ctggataagt tgtacctgcc gctcgtatcg gcgtattttc
     2401 gattagaaga ctttttgtcc taaatgtgga tttaagttag ggtattacca agtataaaaa
     2461 attatgcata ttgagagaac gccgcgtgag tgataacaaa aacaaactct tgctgaacgt
     2521 atcaaatttg aaaacgcact ttttcaccga agatggcgtg gtgaaagccg tggatgacgt
     2581 gagtttttat gtgaatgagg gcgaaatatt gggtttagtg ggtgaatccg gttgcggaaa
     2641 gaccgtcact tcactctcta tcattgggtt gctttcaaaa cccggaaaga ttgttggtgg
     2701 cgaaatccag tatgatggac agaacttgct gaacttatct tacgagcagt tatccgaaat
     2761 acgcggaaac cggatttcaa tgatcttcca acaaccacaa accagcttga atccggtgct
     2821 cacggttggc gaccagatag cagaggtgct tgaaatccac cagggtgtga gtaccaaaga
     2881 tgcatggggg cacgcggttg agatgttgcg ccaggtggga atcccctcgc ccgagaaaag
     2941 ggcctttgct tatcctcatg aattgtctgg cggacaggct cagcgggtaa tgattgccat
     3001 ggcattaata ttagaaccga aactgttgat tgccgatgag cctacaactg ccctggatgt
     3061 gaccatccag gctcaaattc tcgacctgat gcgtggcttg aataacataa tggggaccgc
     3121 gattgtttta attacccatg acttgggcgt gattgcgggg atcgcaaacc gcgtggcggt
     3181 gatgtacgct ggaaagattg ttgaacacac gatggttgag acattatttg aggatcctct
     3241 tcatccatat acgaagggtt taattaattc gattccagta ttggggcggg tcaaagatcg
     3301 attggaagta attcctgggg tggttcccaa cctgatcgac ttgcccaggg gatgtgcgtt
     3361 tgcaccccgt tgtgaggcga gaattgaaca tgggctggaa atttgtgaag agaagatgcc
     3421 cgagatggtg caaattgaga ggggacatga ggtgcgttgc tggctgtatc cggactaaag
     3481 cggcaccaca agcagtcata gacatcaaat gagtcgctag gcaatagatg cttcacacaa
     3541 gccagtcaga ctggctttga ccccagcgac taatatagcc ctgttttcac ccatattgat
     3601 aaccgctctc gccatccaga ggagaccgcc tcaccctctg gatgactgac atataataaa
     3661 gagcagtttt gtgctacgtg aaaggagcct aagatggacg tggtggagct ctaccaacga
     3721 agagcaccta ttgaaatagc ccctgaggat tttcggaaag ttggatatca agttgttgat
     3781 caattagcca attttcttga atgcttacct cagctacctg tcacgactgg agaaactccc
     3841 acccaaatca gaaaaatact tggagaaaat actcttcctg atgatgggga ttctctggaa
     3901 tgtgtgctcg actaaaccat tcggttgttg gttaatcatt ccctatttaa tgggcactca
     3961 cggttttggg gatatataac atcatcggca gcaccaattg gggcattggc agatatgatt
     4021 gcggcgatga ttaatccaaa cgtgggcgct tggatatcat cgccaatggc atcggaaatt
     4081 gaggcacaaa ccgtccgttg gatcgccgag atgcttggtt atccaccgga ttgcggtggt
     4141 ctgtctatct ttctccggtc cgcaaacctc taaggcttaa tgtttacggt catttcttgg
     4201 attgtctgac aaacaggggc gcgaaacccg cgattaatcc tatcaacgcg ccgaggataa
     4261 aacctatcgg accaccggct tcataacctt gcctgccgtt gaattcaagg ccaaaaatta
     4321 tgaagatgtt tgaaccagca atgaagccaa ttacagcaga agccaatcca atgaggatta
     4381 aggcaagaac acgaaggact atgttgatca tttgcggtcc tcctttgatt gcaaacaggc
     4441 gcaatcacca atcaaccaaa ttccggatga atggttattg cttaatttga ctgccaaata
     4501 accagtaacc acttccaact aaacccctga aagcgccgag aataaaacct atttgcccag
     4561 tggcctcata ttctactctg ccgttgaaaa cgaattcgtg tccgaacagg atttcataga
     4621 tagcggctaa attcccaccg attattgcac caaggatcaa gccaatgacg gcggatacaa
     4681 atccaatcag gagtatggca agaatacgaa gcgcgatgtt aatcattaac atggtcattt
     4741 tcgatcttcc tttagctacg aatggaggta gccgctatca atcaaccaaa atttgttcag
     4801 atagtccttg atctgtaagc ttcaaatcaa aagagcggca aaatcccttg taatgtcatt
     4861 cgagtgacac atctgtgtcg atattagtta ttagctatag agacgcagag tgaacacgat
     4921 aagagttgtt atcaagaccc acgcaaaaag aaatcctgcc ccgcataata agagtgtgcc
     4981 tgctattagg tggttaacac gcaggcggtt accccaagaa gccatcacac
//
LOCUS       DBNGSX010000269         7062 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1684035, whole genome shotgun sequence.
ACCESSION   DBNGSX010000269 DBNGSX010000000
VERSION     DBNGSX010000269.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7062)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7062)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7062
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1684035"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..920
                     /gene="mtnP"
                     /locus_tag="AC16CB_08190"
     CDS             39..920
                     /gene="mtnP"
                     /locus_tag="AC16CB_08190"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0033353 - L-methionine cycle [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HIR3342487.1"
                     /translation="MNPPTLAVIGGSGLYAMPGLKDTQEIDLTTPYGKTSAPIILGTL
                     EGQRIAFLARHGIGHHISPSEVPYQANIYALKTLGVERITSISACGSLRDDYAPGEIV
                     VPDQVFDFTRGRARSFFGEGLVAHISVADPICPDFSQQVYEAVVAAGGKVHQGGAFIT
                     IEGPRFSTKAESHIYRSWGMSIIGMTTSPEVFLAREAEICYAVMAHVTDYDVWHTSEE
                     PVTVEMVIETLHRNTQLAQEAVRILARELKPERSCSCPDALASALITQPDKIPAETRQ
                     KLAIFVDKYLERSGDKG"
     gene            1015..3384
                     /locus_tag="AC16CB_08195"
     CDS             1015..3384
                     /locus_tag="AC16CB_08195"
                     /inference="COORDINATES: protein motif:HMM:NF013279.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR3342488.1"
                     /translation="MIVLAVVFVLLFSASLTLAPAARARSWQVDYPWEHWLGVLIWAG
                     CIIAAHLEINRRLPERDPLLLPLAALLSGWGLMTIWRLVPAVGLRQSLWLLVSGAVFT
                     LGLRLPRDLGFMRRYKYVWLTGGLLLTALTLILGTNPLGYGPRLWLGCCGLYFQPSEP
                     LKLLLITYLAAYLAEMDPFNLPEKQRFMLPWLAPTLLMAGLALSLLLVQRDLGTSSIF
                     IYLYVAVVFIATGNRRIVLYGGLTLLVAGITGYALFDVVRLRVDAWINPWLDPSGRSY
                     QIVQSLLAVANGGLFGRGPGLGYPTLVPISHSDFIFAAIAEENGLMGALVLLLLLALF
                     ALRGLRIALRAPDTFRRYLAAGLTAHLVGQSILIIGGNMRLLPLTGVTLPFISYGGSS
                     LLTSFISLLFLELIGSGEEDHEPAPLPQARPYRWMGAFLLVGLGTLALAGGWWAFIRS
                     PALLARNDNARRFIADRYVQRGALLDRRNTPLVATYGEPGTYSRSVVYPLLSSVIGYS
                     NPIYGQSGLEASLNDYLRGLRGNPGLTTWWNYLLYGQHPPGLDVRLSLDLHIQRAADE
                     NLGEHIGAIVLLNSLSGEILAMATHPTFDANVLDDTWENLVGDQRAPLLNRATQGSYP
                     VGDLENVLIPQAAELAGLDISSAIYLPAADSPLPGESLALSPLQVALTAAAVNNGGKL
                     PIPNLVMAVNTPAAGWVVLQSHEKSTSIFSAEESITKAQALAVESQPVWQITTSAADD
                     AGQFVSWFVGGTLPEWSGAPLAIAVVLEENNPQLAEEIGRNVLQQAMMP"
     gene            complement(3447..4670)
                     /gene="tyrS"
                     /locus_tag="AC16CB_08200"
     CDS             complement(3447..4670)
                     /gene="tyrS"
                     /locus_tag="AC16CB_08200"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR3342489.1"
                     /translation="MPEMTIDDQVQLLMQGTEYGDEELKQAMATELRQRLVEAKAEGR
                     SLRVYCGFDPRTSDLHLGHMVPMRKLRQFQELGHEVTFLVGNYTSLIGDPSDKDKLRP
                     ILTPEQVEENARTYAEQAYKILDKQKTLIRYNATWLSQLTFAELIKLASNFTLQQFLT
                     RENFRLRWDKGDPIYLHETFYSIMQGYDAYALRTDVQVGGTDQLFNIVTAARKVMSYL
                     DEKPNIAIILGILPGTDGEVKMSKSLGNHIPLLSTPEDMYGKVMSVPDKAMGLYFRLV
                     TRWTPPEIEEIESGMASGQLHPRDIKMKLAHEIVSIFQSEQAAERAQEAFVRVFQRGN
                     LPTYMPEFSLQDGQTVLDVLVAGNLVSSKSDGRRMIDQQGVRLDGEVLTDPNQPLTQP
                     GVLQVGKRKYLRLVR"
     gene            complement(4883..5392)
                     /locus_tag="AC16CB_08205"
     CDS             complement(4883..5392)
                     /locus_tag="AC16CB_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342490.1"
                     /translation="MITPDREYHPELIPRRGEWIAWGSTLVVSAAWIILRLSGQSVFV
                     AVPFLAITLLLVAMGISLGNWMDRRTVIHLGDDGVKFDNGLRNVNLSWPEVHQVRVLP
                     SRFGKKVQVFGEQVYFSFRTLGEVHLQGELKGRMGFEKGEEILRQIVLMSGLQIMERE
                     GEEIYYIRP"
     gene            5503..6006
                     /locus_tag="AC16CB_08210"
     CDS             5503..6006
                     /locus_tag="AC16CB_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR3342491.1"
                     /translation="MHAPVLVLNANFEPLNVCTTRRAIGLILSGKADLVLNGRGVIKT
                     VSTAYPRPSIIRLERMVKRPRPRIRLTKREILRRDDFTCQYCGQNSPHLTVDHIVPRR
                     LGGLHSWDNLVAACPPCNHRKGGRTLEQAQMHLLRIPSEPSSSAQYLFARHLNENIEW
                     VPFVNDW"
     gene            complement(6079..6834)
                     /locus_tag="AC16CB_08215"
     CDS             complement(6079..6834)
                     /locus_tag="AC16CB_08215"
                     /inference="COORDINATES: protein motif:HMM:NF049367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3342492.1"
                     /translation="MAKLIILGSSNAIPDEKHENTHMALSGRERTVLIDCVSNPMVRL
                     PKAGLEFDNLTDIILTHFHPDHVSGVPLLLMCTWLQGRKRPLNIYGLGYTLDRVEKMM
                     EFYDWATWPHFFPISFHRLPEAEMTPVIDCDEFRIFASPVHHLIPNIGLRVEFLETGK
                     TMAYSCDTEPCREVVRLGSGADVLIHECSGATLGHTSAEQAGEIAQRAEAGALYLIHY
                     PTGKNDPGKLVPDAKKMFNGPVALAEDFMTLEF"
     gene            complement(6868..>7062)
                     /locus_tag="AC16CB_08220"
     CDS             complement(6868..>7062)
                     /locus_tag="AC16CB_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342493.1"
                     /translation="GASILRLGATLAAPSQGETIGEAWLRSLADWAKIMLGLVIPLLL
                     GAAILEVLVTPRLAIWMFGG"
BASE COUNT         1612 a         2015 c         1780 g         1655 t
ORIGIN      
        1 ggacaaataa cactcatggt cacgattgga gagtcctcat gaacccaccc accctggcag
       61 ttatcggcgg gtcgggcctg tatgcgatgc ccggcctgaa agacacacaa gaaatcgatc
      121 tgaccacacc ttacggtaaa accagcgctc cgatcatctt gggaaccctg gaaggtcagc
      181 ggatcgcctt tctggcgcga catgggattg gacatcatat ctcgccctca gaagtcccct
      241 accaagccaa tatctacgcc ctgaaaaccc tgggggttga gcgcatcacc agtatcagcg
      301 cctgcggctc tctacgcgat gattacgctc cgggtgagat cgtggtgccc gatcaggtgt
      361 ttgatttcac acgcggcaga gcgcgcagtt tcttcgggga gggtttggtg gcgcacatca
      421 gcgtcgctga cccaatctgc ccagatttct cccaacaggt atatgaggcc gtcgtagctg
      481 ctgggggtaa ggtacaccag ggcggggcat ttatcaccat cgaaggaccg cgattctcaa
      541 ccaaggctga atcgcatatc taccgctctt ggggtatgtc aatcattggc atgaccactt
      601 ctcccgaggt ctttctcgcc cgcgaggctg aaatctgcta tgcagtgatg gcgcatgtca
      661 cagactacga tgtctggcac accagcgagg aacctgttac cgtggagatg gtcattgaaa
      721 ccctgcaccg caacacgcag cttgcccagg aggctgtgcg tattctggct cgcgagctga
      781 agcctgagcg ctcatgcagc tgcccagatg ccctggcgtc tgcgttaatt acccagccag
      841 acaagattcc tgccgagaca cgccagaagc tggccatctt tgtcgacaaa tacctggaga
      901 gatcagggga taagggataa gggttgcccc cctatcccct acctcctctc tcttcctccc
      961 taaaccttgc tatcccttca aaatccccca tcaaccaacc gtaccgaaag aggcttgatt
     1021 gtcctggctg tagtgtttgt gctgctcttt agcgcctcgc tgaccctagc tcccgcggct
     1081 cgggcgcgct cctggcaggt ggattacccc tgggagcact ggctgggtgt gctcatttgg
     1141 gcaggttgca tcatagcggc acacctggag atcaaccgca ggctgccaga acgcgacccc
     1201 cttcttctgc cactagctgc attattgagc ggctggggat tgatgacaat ctggcgacta
     1261 gtccccgcag ttggtctgcg ccagagcttg tggttactgg tatccggcgc ggtatttacg
     1321 cttggactgc gcctgcccag agacctggga ttcatgcggc gatataaata cgtttggcta
     1381 accggtgggc tgctgcttac agccctgact cttatcctgg gcaccaatcc gctgggatac
     1441 ggcccgcgtt tatggttggg ctgctgcggg ctatactttc aaccctctga gccgctcaaa
     1501 ctgctgctga tcacctacct ggcagcctac ctggcagaga tggatccgtt caatctgccc
     1561 gaaaagcagc gtttcatgct gccctggctg gcgcccactc tgctgatggc aggattagcg
     1621 ttatccctac ttttagttca gcgcgatttg ggaacatcat caatatttat ctacctctac
     1681 gtcgcagtgg tattcatcgc cacgggtaac cgccgcatag tgctgtatgg cggcctcacg
     1741 ctgctggtgg ccggcataac gggttacgcc ctattcgatg tggtgcgcct gcgcgttgac
     1801 gcctggatta acccctggct cgacccaagc gggcggtcat atcaaattgt tcaatctttg
     1861 ctggcggtag caaacggtgg tttattcggg cgcggtccgg gcttgggata ccccaccctg
     1921 gtacccatat cgcattcgga cttcatcttc gccgcaattg ccgaggaaaa cggcctcatg
     1981 ggagcgctgg tcttgttact cctgctggca ttattcgccc tgcgcggtct gcgcattgca
     2041 ttgagagctc cagatacttt tcggcgttat ctggcggctg gattaaccgc tcacctagtg
     2101 gggcaaagca tattgattat cggcggtaac atgcgtttat tgccgttgac cggagtcaca
     2161 ctcccgttca tatcctacgg cggatcttcg ctgttaacgt cttttatttc actgctcttt
     2221 ctggagctga tcggcagtgg agaggaagat catgagcctg caccgcttcc ccaggcgcgt
     2281 ccatatcgct ggatgggagc ctttctattg gttggattgg ggaccctggc tcttgcaggc
     2341 ggttggtggg cttttatccg tagccccgct ttgcttgccc gcaatgataa cgcgcgccgc
     2401 ttcatcgccg atcgctatgt gcaacgcggt gctcttctag accgccgcaa taccccgctg
     2461 gtagcgacat atggggagcc aggcacgtat tctcgctcgg tggtataccc acttctcagc
     2521 tctgtgatag gctactcgaa tccgatctac ggacaatcgg ggttagaagc cagcctcaat
     2581 gattacctgc gcggtctgcg cggcaaccct ggacttacca cctggtggaa ctatctgctc
     2641 tatgggcaac atccgcctgg gctcgacgta cgcctgagcc tggatctcca catccagcga
     2701 gctgccgacg aaaatttggg cgagcatatt ggcgcgattg tcctgctgaa tagcctgagt
     2761 ggcgaaatcc tggcgatggc aactcatcca acctttgacg caaatgtgct agatgatacc
     2821 tgggaaaacc tggttggcga ccaacgcgcc ccgctgctga atcgcgctac gcaagggtcg
     2881 taccctgtcg gcgacctgga aaatgtgctc atcccccagg ctgcggaact tgcagggctg
     2941 gatatcagct cagccatcta tctaccggct gctgactcac ccttgccagg ggagtccctt
     3001 gcgctcagcc cgctacaggt ggcgctcacc gccgcggcgg ttaacaacgg cggcaaattg
     3061 cccatcccca acttggtgat ggcggtcaat acaccggcag caggatgggt cgttctccaa
     3121 tcacatgaaa aaagcacatc catttttagc gccgaggagt ccataactaa agcgcaagca
     3181 ctggcggtag aatctcagcc tgtttggcag ataaccacct ccgcagccga tgatgccggg
     3241 caattcgtat cctggtttgt gggtggcaca ctgcccgaat ggagtggtgc accactggct
     3301 atagcggttg tactagaaga gaacaatcca cagctagcag aagaaatagg tagaaacgtg
     3361 cttcaacaag ccatgatgcc ttgacggatt attcaccccc acctgcgctt ggacgatggc
     3421 tccgctttta aacccacctt tagacattaa cgcacaagcc taagatactt cctcttacct
     3481 acctgtaaaa ccccgggctg tgtgaggggc tgattaggat ccgtcaaaac ttcgccatcc
     3541 aagcgcaccc cctgttgatc tatcatgcgg cgcccatcac ttttgctgct taccagattg
     3601 cctgcaacca gcacgtccaa gaccgtctga ccatcctgca ggctaaactc aggcatatac
     3661 gttggaaggt tgccgcgctg aaaaacgcgc acgaaggctt cttgggcgcg ctcggcggcc
     3721 tgctcacttt gaaagatgga aacaatttcg tgggcgagct tcatctttat atcgcgcggg
     3781 tgaagctgac ctgatgccat gcccgattct atctcctcaa tttcgggcgg agtccagcgc
     3841 gtcaccaggc ggaagtacag gcccatggct ttatctggca cgctcatcac ttttccatac
     3901 atatcctcag gtgtggagag cagggggata tggtttccca atgacttgct cattttgacc
     3961 tcaccatccg tgcctggaag tatgcctagt atgatggcaa tattgggttt ctcatccaga
     4021 taactcatca ccttacgagc tgccgtgacg atattgaaca actggtctgt gccccccacc
     4081 tgcacatctg tgcgcaaggc ataggcatcg tacccttgca tgatagagta gaatgtttca
     4141 tgcagataaa tgggatcgcc cttatcccag cgcaggcgaa aattctcccg cgtgagaaat
     4201 tgttgcaggg tgaagttcga ggccaattta attaactcgg caaaagtcag ctgcgaaagc
     4261 caggtagcgt tgtagcggat caaagtcttt tgcttgtcca gtattttata cgcctgctcg
     4321 gcgtaggtgc gggcgttctc ctccacctgc tcgggtgtca atatggggcg caacttgtct
     4381 ttatcggagg gatctccgat gagcgaggtg taattgccga ccagaaatgt gacttcgtgc
     4441 ccgagctctt gaaactggcg caatttgcgc atgggcacca tatgaccaag atgcaaatca
     4501 ctcgtccttg ggtcaaaacc acaatagacg cgcaacgacc gaccctcagc tttggcttcg
     4561 accagccgct ggcgcagttc ggtagccata gcttgcttta attcttcatc gccatattca
     4621 gtgccttgca tcagaagctg gacttgatca tcaattgtca tctctggcat atttcttaag
     4681 ccctccaaac tatcttggat atcttcacta ggttcctaag ctacagtgcc tgttatcgct
     4741 aaataacagg agtttcgcaa ttgcaagggt ggtcgggctt cacccgccgc aacccgcctt
     4801 ctgccttttt caagcaggaa agtcatcaac aataaaccgc gccctctgcg gtgagggcgc
     4861 gggaactcac cacaaaagtc actcatgggc gaatataata aatttcttca ccctcacgct
     4921 ccatgatttg tagcccactc ataaggacga tctggcgcaa tatctcttca cctttctcaa
     4981 agcccatcct gcctttcaat tcaccctgaa gatgtacctc tcccaatgtg cggaaggaaa
     5041 agtatacctg ttcgccaaaa acctggactt tttttccaaa acgggaaggg agcactcgca
     5101 cctggtggac ttcgggccag gaaaggttaa cattgcgcaa gccattgtca aacttcactc
     5161 catcgtcacc cagatgaatc accgttcggc ggtccatcca gttgcccagg gaaataccca
     5221 ttgctacgag caacagggta attgccaaaa aaggcacggc aacaaaaacc gattgcccag
     5281 ataagcgcag aataatccag gcggcactca caaccagcgt tgaaccccag gcgatccact
     5341 ccccccgtcg cgggatcaat tctggatgat actcacggtc aggtgtaatc acgacaagta
     5401 attataacag caggcctttc gagtgtatac aaaaaatgcc aggcgcttat tgacagcatc
     5461 atagcatccc gtgctataat tccgagagtt acggaaagcc ctatgcatgc acccgtactc
     5521 gtcctaaatg ccaactttga gccactcaac gtctgcacaa cgcgtcgcgc gattggcttg
     5581 atattgagtg gtaaggcgga tctggtgttg aacggtcgtg gggtaatcaa gacagtatca
     5641 actgcctacc cgcgaccatc aattatccgg ctggagagga tggtcaaacg gcctcggccg
     5701 cgcatccgct tgacaaagcg cgaaatcctg cgcagggacg atttcacttg ccagtattgc
     5761 gggcaaaact ctcctcacct aaccgtagat catattgtgc ctcgacgttt gggaggtctg
     5821 cactcatggg ataatctggt agctgcctgc ccaccctgca atcatcgcaa aggggggcgc
     5881 acgcttgagc aagctcaaat gcacttacta cgcataccaa gcgaaccttc gtcctccgca
     5941 caatacttgt ttgcacgcca cctgaatgag aatatcgagt gggttccctt cgtcaacgac
     6001 tggtaggaaa aaagcaccga aacgaatcaa aaatggggtt ttttagaaac cccatttttt
     6061 gtttaacgcc gatgtatatt aaaattccaa ggtcataaag tcttcagcca gggccactgg
     6121 cccgttgaac atcttctttg cgtcggggac aagctttccg gggtcatttt tccccgtcgg
     6181 gtaatggatc aaatacagcg ctccggcctc cgccctctgg gcgatctccc cagcttgctc
     6241 cgcagaggtg tgtcctaaag tagcccctga acactcgtgt atgagcacat ctgcaccaga
     6301 accaagccgc accacctccc gacaaggctc tgtatcgcat gaatacgcca tggttttacc
     6361 cgtctctaaa aattccactc gcaatccaat atttggtatc agatggtgca ccggagaggc
     6421 aaaaatgcgg aactcatcgc aatcaataac tggtgtcatc tctgcctcag gcaggcggtg
     6481 gaaagagatc gggaagaaat gcggccaggt cgcccagtcg taaaactcca tcatcttctc
     6541 cacgcggtcc agcgtatagc ccaagccgta gatattcaat gggcgttttc gcccttgaag
     6601 ccaggtgcac atcagtaata gaggcacacc cgagacatga tccgggtgaa aatgggtaag
     6661 aatgatatct gtcagattat caaattccaa cccagctttg ggcaatctaa ccatcggatt
     6721 actaacacaa tcaatcagca ccgttcgttc acgaccggac agggccatat gggtattttc
     6781 gtgtttctcg tctggtatgg cgtttgatga acccaggatg atcaactttg ccacagcaaa
     6841 taccctccgc aattcttcag gctgaggtca gcccccgaac atccaaattg ctagacgtgg
     6901 agtaactaac acttcaagga tcgccgctcc cagaagaagg ggtatcacta gacccagcat
     6961 gattttagcc caatctgcca gtgaacgcaa ccatgcctcg ccgatagtct cgccctgaga
     7021 aggcgctgcc agggtagctc ccaggcgcaa aatggatgct cc
//
LOCUS       DBNGSX010000270         2509 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_228922, whole genome shotgun sequence.
ACCESSION   DBNGSX010000270 DBNGSX010000000
VERSION     DBNGSX010000270.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_228922"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /gene="argH"
                     /locus_tag="AC16CB_08225"
     CDS             complement(<1..1117)
                     /gene="argH"
                     /locus_tag="AC16CB_08225"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR3342494.1"
                     /translation="MTLWGGRFRGKLDPNAWSLNASLRIDQRLAPQDVRGSLAWAKAL
                     EIAGVLTMGESAQIQHGLERIGEELHSGVFTFLESDEDIHTAVERRLGELIGPLAGKL
                     HTGRSRNDQVVTDFRLWLLENLPALGTAIANLQAALLQRAESDLGTLMPGYTHLQRAQ
                     PILLSHWWLSHFWPLARDRDRLQDLAGRTAVLPLGAGALAGAPFPVDRIDLAQELGFD
                     HPAPNSIDAVSDRDFAAEFLFCAALTGVHLSRMAEGVILFSSAEYGFFELSDAFATGS
                     SLMPQKKNPDVFELARGKTGSLIGWLSGLMATLKGLPSAYDKDLQEDKAPVFSAYDTL
                     MSLLPVMGGALRTLTIHPERLRSAIDAGMLATDLADYL"
     gene            complement(1173..2432)
                     /locus_tag="AC16CB_08230"
     CDS             complement(1173..2432)
                     /locus_tag="AC16CB_08230"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR3342495.1"
                     /translation="MIKPTVKKVVLAYSGGLDTSVIVPWLKENYGCEVVCFTADIGQI
                     EELDGLEAKALASGASQLIIHDMREEFASEYIFPVLLAGAVYERKYLLGTSFARPLIA
                     KHQVEVAHEVGADAVAHGATGKGNDQVRFELTYTALDPRLKVIAPWREWDIRSREDAI
                     AYASAHNIPVTATVKSIYSRDRNLWHLSHEGGPLEDPWREPDEDMFQLSISPEEAPEQ
                     PEFVEIMFDSGTPVAVNGQKMTPAAMVTKLNKLGGAHGIGRADLVESRLVGMKSHGVY
                     ETPGGAILLAAHRELESLVLDRDTIQYKDMVALKYAELVYNGMWFSPLREALAAFVDS
                     TQGPVTGVVRLKLYKGNIIPAGRKSPFSMYREDFATFGQEDVYDQSDAEGFIHLYGLP
                     LKVRAMNGLPISGLNMPKPDYSRFKRD"
BASE COUNT          504 a          724 c          723 g          558 t
ORIGIN      
        1 ccaagtaatc tgccagatcg gttgccagca taccggcatc gatggcggag cgcaggcgct
       61 caggatggat ggtaagcgtg cgcagggcgc ctcccatcac cggcagtaga gacatcaggg
      121 tgtcgtaagc gctgaagaca ggcgctttgt cttcctgcaa atctttgtcg taggcagatg
      181 gcaggccttt gagggttgcc atcagtccgc tcagccagcc tatcaggctg cctgtcttgc
      241 cgcgcgccag ttcaaacacg tccgggttct ttttctgcgg catcaggctg gagccggtgg
      301 caaacgcatc ggatagttcg aaaaagccgt actccgctga ggagaacagg attacgcctt
      361 ccgccatccg gctgaggtgc acgcccgtca atgctgcgca gaataaaaac tcggcagcga
      421 agtcgcggtc agagacagcg tcgatgctat taggggcggg atgatcgaag cccagctctt
      481 gtgctaagtc gatgcggtct actgggaatg gcgctccagc cagcgcccct gctcccaggg
      541 ggagcacagc ggtgcgtcct gccaggtctt gcaggcggtc ccggtcgcga gccagcggcc
      601 aaaagtgaga tagccaccag tggctgagca ggatcggctg ggcgcgctgc aggtgggtgt
      661 agcctggcat cagggtgccc aaatcggatt ccgcgcgctg aagcaacgcc gcttgcaggt
      721 tggctatggc agtccccaag gcgggcaggt tctccagcag ccacagacga aaatcggtca
      781 ccacttgatc gttacggctg cgaccggtgt gcagcttgcc cgccagtggg ccgatcaact
      841 cacccaaccg tctttctacc gcggtatgga tatcctcatc actctccagg aaggtaaaca
      901 cgccgctatg cagctcttcg ccaattcttt ctaagccatg ttggatctgt gcgctctcgc
      961 ccattgtcaa tacgccggct atttcaagcg cttttgccca agctaggctg ccgcgtacat
     1021 cttggggtgc aaggcgctgg tcaatgcgta gcgatgcgtt cagagaccag gcgttaggat
     1081 cgagcttacc tcgaaaacga cctccccata atgtcatgat attctctctc ttccttaaca
     1141 ggattacatg gtgattggat aagtggctaa atctagtctc gcttgaatct cgagtaatcc
     1201 ggtttgggca tattgagtcc tgagatgggt aatccattca tggcacgcac tttcaggggt
     1261 aagccgtata gatggatgaa gccttctgcg tcggattggt cgtagacatc ctcttgccca
     1321 aaggtggcaa agtcctctcg gtacatgctg aagggactct ttcgtccagc cgggatgatg
     1381 ttgcctttat acagcttcag gcgcaccaca ccggtgaccg gtccctgcgt gctatccaca
     1441 aaggctgcca gagcctcgcg caaaggtgag aaccacatgc cgttgtatac cagttcggcg
     1501 tatttcaatg ctaccatgtc cttgtactgg atggtatctc tgtccagcac cagcgattca
     1561 agctcgcggt gagctgccag caggatggca ccaccgggcg tttcataaac accatgggat
     1621 ttcatcccca ccaggcggct ttccaccagg tcagcgcggc cgatgccgtg cgctcccccc
     1681 agtttgttca atttggtcac catggcggca ggggtcatct tttgcccgtt cactgctacc
     1741 ggagtgccgc tatcgaacat aatctcaaca aactcgggct gttccggggc ttcctccggc
     1801 gagatgctga gctggaacat gtcttcgtcc ggctcccgcc agggatcttc cagggggcct
     1861 ccttcgtgtg agaggtgcca taagttgcga tcccggctgt agatcgactt gaccgtggca
     1921 gtcacgggga tattatgggc gctggcgtag gcgatggcat cctctcgcga gcggatatcc
     1981 cactcgcgcc agggtgcgat caccttcaaa cgggggtcaa gggcagtgta ggtgagctcg
     2041 aaacgcacct ggtcgttgcc tttgccggta gcgccgtggg ctacggcatc tgctcccact
     2101 tcatgggcca cctccacctg atgcttggcg atgagaggcc gggcgaagga cgtgcccagc
     2161 aggtacttgc gctcatatac tgcgccggcg agcagcaccg ggaagatata ctcggatgca
     2221 aattcctcgc gcatgtcgtg gatgataagc tggctggctc cgctggcgag cgccttcgcc
     2281 tccagcccat ccagctcttc gatctgaccg atatctgcgg tgaagcacac cacttcgcag
     2341 ccgtagtttt ccttgagcca gggcactatc accgatgtgt ccaggccacc ggaataagct
     2401 aagaccactt tcttgacagt tggttttatc atgacaacct ccgttctaag attttcgtgg
     2461 gagacgggtt aggccgaaac ccgcgttttg gataaaaaaa ccctcctgg
//
LOCUS       DBNGSX010000271        10180 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_298574, whole genome shotgun sequence.
ACCESSION   DBNGSX010000271 DBNGSX010000000
VERSION     DBNGSX010000271.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10180)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10180)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10180
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_298574"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="AC16CB_08235"
     CDS             complement(<1..1082)
                     /locus_tag="AC16CB_08235"
                     /inference="COORDINATES: protein motif:HMM:NF013955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HIR3342496.1"
                     /translation="MSSIKNILTAKTLKTQSTQPMNKTSKIIQIGMPLLLVIALFGQT
                     TPALAQLAVSSVQPSTVVNITDVNLVITGAEFQDGASVVLQGLGALSTTFISSTVLNA
                     ELPAGASPGVYTVTVVNPDTTSASLDNAITILEPTATLAPATPTATQVASTTRPLIVV
                     DTYSASEDTIYAGQEFNLQIRLKNVGEDTALNLVATFTPGDFVPRKSGGVQAISEIDP
                     DEKKKITQALTASPDLTNKKIGALVMQVSYMDRDGTSYTETFNLSLPVISYGSGVAAT
                     STPTPTSPASNRPQLVITAYGTDVPVLQPGFRVQLHLQVNNLGNMDAKRVSMILGGGS
                     STGGSVDGTPEAGGISGASGDFGDFAP"
     gene            complement(1116..1793)
                     /locus_tag="AC16CB_08240"
     CDS             complement(1116..1793)
                     /locus_tag="AC16CB_08240"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3342497.1"
                     /translation="MEPFIRISGLRKTYKMGRVAVHALDGVDLDIPPNSFLVVMGPSG
                     SGKSTLLHLLGGLDRPTAGHIQVNGESLETLDENALAVFRRRTVGFIFQAFNLIPSMT
                     AQENVAFPMQFARVPRRQRKQRSMEVLKLVGLDDRASHRPTELSGGQQQRVAIARALI
                     NNPHLILADEPTGNLDTTSGASILQVLSGLHQSGRTVVVVTHDVRMTEAATHVVNLLD
                     GRIVDGA"
     gene            complement(1818..2597)
                     /locus_tag="AC16CB_08245"
     CDS             complement(1818..2597)
                     /locus_tag="AC16CB_08245"
                     /inference="COORDINATES: protein motif:HMM:NF016764.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HIR3342498.1"
                     /translation="MAELEIAQTDTPGRFQFNWVPEVLFRPRRAFAKITAQNHGVWLT
                     PLLILTLMMVARVLASGWVRQNFAMTGELPLPPDFQYYSPEQQAQFMQAMQATQGPVF
                     IYVFPLISGVLGIWLGWLLVGGLLHLVTTMLGGRGDTMSAMNLVAWASLPFAVREGVR
                     VAALVISRQLIENPGLSGFAPIDSSGIMAYLSAWLPLVDLYLIWHIILLVLGGRAANS
                     LTPGKAMSGVVLTMLVILGLQALVSFLSTQLGSLTIIRPFF"
     gene            3227..4240
                     /locus_tag="AC16CB_08250"
     CDS             3227..4240
                     /locus_tag="AC16CB_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007143367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HIR3342499.1"
                     /translation="MKIIAEGKGELIGWHDPDENRRWIRENKPRDLRDKRMSVKEAVA
                     QFVHDGDFIAFGGFGHIRIPMALVYEIVRQRKKNLVMAGKTAVHDIDILIGAGCVNKV
                     EVAYAFGHELRGLSPAGRHAVEAGQCKVVAEISNAAYQWRFLAGMMGIPFIPSRNMLG
                     TDTWTYSACKVVEDPWSGKPVCLIPAAYPDVAMFHVPRCDKYGNAQIEGIIVEDFELA
                     RAARRVIVTTEEVVDEEVIRAEPHRTVIPFYLVDALCEVPYGAHPTQMPYLYFFDEEH
                     IGEWFTLAETLEGIEAYFQKYVYGVCDFEEYLERVGGIRKLNYLKRVMQFKEPMRAPW
                     LKG"
     gene            4349..5125
                     /locus_tag="AC16CB_08255"
     CDS             4349..5125
                     /locus_tag="AC16CB_08255"
                     /EC_number="2.8.3.-"
                     /EC_number="4.1.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003332833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase subunit beta"
                     /protein_id="HIR3342500.1"
                     /translation="MTVTTAAYTSTELLACVAAKLLEDKKSVLVGTGLPMIAALLAQK
                     THAPNLLLIFEAGGIGPRMSQMPISVGDILTFYKAVAATSMHDVMSASQAGYVDYGFV
                     GAAMIDMYGNINTTVIGDWERPKTRLPGSGGANDLGSFCHRTIIIMRQDKRRFVEKVD
                     FITTPGYLDGPGARERAGLPEGTGPYRIITQLGLYSFDEQTKRVKLLALHPGVTLDDV
                     RANTSFEILIPEMVQVTHPPTEEELRLLHEMDPIGVIVRK"
     gene            5500..6144
                     /locus_tag="AC16CB_08260"
     CDS             5500..6144
                     /locus_tag="AC16CB_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005372887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0043934 - sporulation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HIR3342501.1"
                     /translation="MYLPKQFEETQFHVMFELIRAHPLATLVTLSSGGLNANHIPLSL
                     SESPPPYGTLRGHIARANPLWSDLNSDIESLAVFSGPDTYISPSWYATKKETGKVVPT
                     WNYAVVHAYGILRIVDDASWLRSQLEALTIHNEASFSEPWAVSDAPQDFTERLISSIV
                     GVEMIITRLSGKWKVSQNQPPQNQASVVASLKASDIPDAPSMATLVETRSKNAR"
     gene            6202..6591
                     /locus_tag="AC16CB_08265"
     CDS             6202..6591
                     /locus_tag="AC16CB_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HIR3342502.1"
                     /translation="MRTDQTATTNIDEYMAGFPNEVREILEKIRMTIREVAPDADETI
                     KYGIPTFTLKGNLVHFAAYKKHIGFYPTPSGIEEFKIELSAYEGAKGSVKFPIDKTIP
                     YELIRKIVAFRVKENLERAKAKGKKEK"
     gene            7089..7820
                     /locus_tag="AC16CB_08270"
     CDS             7089..7820
                     /locus_tag="AC16CB_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342503.1"
                     /translation="MFYKNLPYVGNILAAISSDRVQYLVFIGLSFVILIFTGVLYYSD
                     NQIFQRFIGKINPILAILLAIILGIILFSFLLSQKWFAIFGKETLDGVIRASVLAAVF
                     GVIMILVDMKIVFPADTNILFPESILFYPAIGFFVEILFHLLPVTVLLLLLNSILKNA
                     NIFWISFLIVALLEPVYQALDMANSSRYPLWAVLYVGLHIYLFNLFQLLIFKKYDFIS
                     MYSFRLVYYLFWHIGWGYIRLRLLF"
     gene            complement(7910..8095)
                     /locus_tag="AC16CB_08275"
     CDS             complement(7910..8095)
                     /locus_tag="AC16CB_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342504.1"
                     /translation="MAASYDLDREPHNNMATDIGSHIQNPQPTNTSHHAIAEFIWGLF
                     IPDESQAMVQEDIVAYQ"
     gene            8094..9149
                     /locus_tag="AC16CB_08280"
     CDS             8094..9149
                     /locus_tag="AC16CB_08280"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR3342505.1"
                     /translation="MDKQTSLFKGRIWRYPFNWLLVAIFLSIMIYLVWGGFQVRDKKS
                     NRLIVYAYSTQEEVLTQGIFPAFEQAWEAETGEDIAIEGLFGPSGTLSLQITEGAPAD
                     IAIFSNQRHIDWLKFNKSVRRDTEPIMIASTPLVIITRPGNSANIADFADLTLPGIKL
                     LHADPGSSGAGEWSVLAEYGSAYLVSGDHDSAEAQVKNIWQNVRLVGSSARANMTLFE
                     LGACDALVTYEQDAYLARERGVPLEIVSPPRTILAMHYAVIVDENVTSHERPVVEAFL
                     DFIQSDQGQQILSRYYLRVTTIESDVLPALIQPFTVEDLGGWTQAHNTLIEDLWMKEI
                     KPYLALESANTLLGTAK"
     gene            complement(9275..>10180)
                     /locus_tag="AC16CB_08285"
     CDS             complement(9275..>10180)
                     /locus_tag="AC16CB_08285"
                     /inference="COORDINATES: protein motif:HMM:NF014453.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR3342506.1"
                     /translation="ILGFLDKKLPAGKKVINHLYCLGSVDELEAIVERYGVEEIILAT
                     SAFSSRDKVLEIFRRYSNTNHVNVRISSGLYEIITTGLTVKDIAFVPLVRINPIRLTK
                     VDRALKLVFDFSLALLGTIFIFPFILLIAIAIKLDSPGPIFSRQRIIGLNGKQFEAFK
                     FRTEHSDEAVNLKAYPRLPAKPTSYQYYRNNPRLTRVGKWLLRTNLDELPKIFNVLKA
                     DISLIGPRPITPVEMDKYARWDMNLLSVRPGMTGLWQSFGRSDLSTSERIRLDMYYIC
                     NWSIWLDLQILFHSLLLAINGKGYY"
BASE COUNT         2516 a         2372 c         2659 g         2633 t
ORIGIN      
        1 ggggcgaagt cgccaaaatc tccgctggcg ccagaaattc cgcctgcttc aggggtgccg
       61 tccacgctac cgcctgtgct gctcccacct cctaagatca tggagacgcg cttggcatcc
      121 atgttgccta gattgttcac ctgcaggtga agctggactc gaaagccggg ctgcagtaca
      181 ggcacgtctg tcccgtaagc ggtgatgacg agctgcgggc ggttgcttgc gggtgaggtg
      241 ggggtgggtg ttgaagtggc tgccactcca ctaccgtatg agatgaccgg cagactgaga
      301 ttgaacgtct ctgtgtagct tgtgccatct ctatccatgt aggagacctg cataaccagc
      361 gcgccgatct tcttgtttgt cagatcgggg ctggcggtta aggcttgggt gatcttcttc
      421 ttttcgtcgg ggtcaatctc gctgatcgcc tgtactccac cgctcttacg ggggacgaaa
      481 tcgcccggtg tgaaggtggc taccaggttc agcgcggtat cttcgccaac attcttgaga
      541 cgaatctgca ggttgaactc ctggccagcg tatattgtgt cttcactggc gctgtaggtg
      601 tcgacaacaa tcagcgggcg ggtggtagat gcgacctgtg tggcagtcgg tgtggccggc
      661 gcgagggtag cggttggctc caggatggtg atggcattat ccaaagaggc tgaggttgta
      721 tcggggttga ccaccgtgac cgtgtaaacg cccggcgatg ccccagcggg cagctcggcg
      781 ttgagcactg tgctgctgat gaaagtggtg ctcaaagccc caagaccctg caacacaacc
      841 gaggcgccgt cctggaactc agcgcctgtt atcaccaggt tcacatccgt gatgttgacc
      901 actgtgcttg gctgcacgct ggaaactgcc agttgtgcta gcgctggggt cgtctgcccg
      961 aacaaagcaa tgaccagaag aaggggcatg ccgatttgaa tgattttaga tgttttattc
     1021 ataggttggg tactctgcgt tttcagcgtc ttggcggtga gaatattttt tatcgaagac
     1081 atgagaccat ccattcccaa aatccgtttt caacattacg caccatccac aatgcgacca
     1141 tccaagagat tgaccacatg agtggcagcc tcggtcatgc gcacatcgtg ggtgaccact
     1201 actacagtgc gaccactctg atgcaaaccg gaaagcactt gcaggatact ggcgccgctc
     1261 gtggtatcca ggttaccggt tggctcgtca gccaggatta aatgcgggtt gttgatcagg
     1321 gcgcgggcga tggccacgcg ctgctgttgc ccgccggaga gctcggtggg gcgatgactg
     1381 gcacggtcat ccagaccgac cagtttaagc acttccatcg agcgttgctt gcgctgtcgg
     1441 cgggggacgc gcgcaaattg catcgggaag gctacattct cttgggcggt catgctgggg
     1501 atcaggttaa acgcctggaa gataaagcct actgttcgcc ggcggaagac tgccagggcg
     1561 ttctcgtcca gagtttctag ggattccccg ttcacctgga tatgtcccgc tgttgggcgg
     1621 tcgagcccgc caagcaagtg cagcagggtg ctctttcccg agccggaagg gcccatcacc
     1681 accagaaagc tgttgggtgg gatatccagg tcgactccat ccagggcgtg cacagccacc
     1741 cgccccattt tgtaggtctt gcgcagaccc gatatacgga taaagggctc catattgctt
     1801 ttagactcga tggtggtcta gaagaaggga cggatgatgg tcaaactgcc caactgagtg
     1861 gataagaaac ttaccagtgc ctggagtccc aggataacca gcatggttaa aactactccg
     1921 ctcattgctt tgcctggtgt caggctattg gcagcgcgcc cgccaagcac cagcaggata
     1981 atgtgccaga tgagataaag atcgaccagc ggcaaccagg cagaaaggta ggccatgatg
     2041 ccggatgaat cgataggagc gaaaccggat aggccgggat tttcgatcag ttggcggctg
     2101 atgaccagcg ccgccacacg caccccttcg cgcacagcaa agggtagact tgcccaggcg
     2161 accaggttca tggctgacat ggtatcgccg cgtcccccca gcatcgttgt caccaggtgg
     2221 agcaagcctc ccaccagcaa ccagcccagc cagataccca gcaccccgct tatcagaggg
     2281 aagacataga tgaaaaccgg gccttgggtg gcctgcatcg cctgcatgaa ttgcgcctgt
     2341 tgttctggag agtagtactg gaagtccggt gggaggggta actcgccggt catggcgaag
     2401 ttctgccgca cccagccaga tgccagcacc cgcgccacca tcatcagggt caggatgagc
     2461 agtggtgtga gccatacccc gtgattttga gccgtgattt tggcgaaggc gcgccgcggc
     2521 ctgaacaata cctcgggtac ccaattaaac tgaaagcgcc cgggcgtatc tgtctgagcg
     2581 atctctagtt cggccatagt ttatcgaatg gctccagtat tttcgttttg ggctagtcca
     2641 aacttacaat tgagtgcaga ttctaccata tctacaagcc tcggaatgat tatgctagtc
     2701 tgtgacgtag ggctgatgca aagtctatat aagccagtca ttaggaactc cacagcgcca
     2761 gcgcttttct ttcgcgcgaa aaatgtcatt tgtgagggtt taagcgctct tgcaatgacg
     2821 cggctttgca ttagccatcc tttatgacga tgcttcgcaa gcaaaagttc ccaataatcg
     2881 ggacaagcaa gttcgggcgc gcgtgtcaac tagcaagcag ctattacatg cagacccgtc
     2941 ttggaacaac cattgattga cttccttgca ggggagcgaa accgcaaatc gttgacttta
     3001 atcgtgaaat ttgttacaaa ttaatttgaa agaccaaatg gattgctagt gtgctcctat
     3061 gctcacacaa gcctaccaaa ctaccggcgt gatttttaag cagtggagca tatagtccgt
     3121 gttgcccagg agctaaatcg agatattgcc aaaccacttc aagctcgtaa aatcctaggc
     3181 ctctcttaag gcaatgtgtg aacgatattg caaggaggca taaatcatga agattattgc
     3241 agaaggcaag ggtgaactga ttggatggca tgaccctgac gagaaccgaa gatggattcg
     3301 ggagaataaa ccgcgcgatc ttcgggacaa gcgtatgagt gtgaaagaag ccgtggcgca
     3361 gtttgtccac gacggcgact ttattgcttt tggtggcttt ggtcacatcc gaattccgat
     3421 ggctctggtt tacgagatcg tccgccaacg aaaaaaaaac ctggttatgg ctggcaagac
     3481 cgctgtccac gatatagata tacttatcgg cgcaggctgt gtgaataaag ttgaggtggc
     3541 ctatgccttt ggtcacgagt tgcgcggtct ttcgccggcg ggccgccatg cggtagaggc
     3601 aggtcagtgt aaagtagtag ccgagatctc gaacgccgcg tatcaatggc gcttcctcgc
     3661 aggcatgatg gggattccat tcatcccctc tcgcaacatg ttgggcactg atacttggac
     3721 atacagcgcg tgcaaggtgg tggaagaccc ctggagcggt aaacctgttt gcttgatacc
     3781 agccgcgtat cccgacgtag ctatgttcca cgtgcctcgc tgtgataagt acgggaatgc
     3841 ccagatagaa ggtattatcg tggaagattt tgaattggcc cgggcagccc gccgcgtcat
     3901 cgtaactacc gaagaagtcg ttgacgaaga ggtgatccgc gccgagcctc accggacggt
     3961 gatccccttt tacttggtag atgcattgtg cgaggtccct tacggtgctc atcccacaca
     4021 gatgccctac ctctactttt tcgacgagga acacattggc gaatggttca cccttgccga
     4081 gacattggag gggattgagg cttactttca gaaatatgtc tatggcgtct gcgactttga
     4141 agaatatcta gagcgggtag gtggtatcag gaagctcaat tatctcaagc gcgtgatgca
     4201 gtttaaggag ccaatgcgag cgccttggct caagggttag ttattggagt taggtaattt
     4261 gtccaaatgc aagtcccgag cgattgcatc ttctttacac tcttcaagcg caatcgtatc
     4321 gtaagtgcgc gaagtgagga ggagatccat gacagtaacc acagcagcgt acacttccac
     4381 cgagctttta gcttgtgtgg cagccaagct tctagaggac aagaaatcgg ttttggtagg
     4441 cactggctta cccatgattg ctgccttatt agctcagaag acgcatgcgc caaatttgtt
     4501 gcttatcttc gaggctggag gaattggccc tcggatgtcc caaatgccca tctctgtggg
     4561 cgatatcctt actttttaca aagctgtcgc cgctaccagc atgcacgatg tcatgagcgc
     4621 ctctcaggcg ggctacgttg attatggttt cgtaggcgca gcgatgattg atatgtatgg
     4681 caacatcaat accaccgtta tcggtgattg ggaacgaccc aagacgcgtt tacccggcag
     4741 tggcggagcg aatgacctgg gttctttttg tcatcgcaca attattatca tgcggcaaga
     4801 taaacgccgc tttgtagaga aggtggactt tatcacgacg cccggttact tagatggtcc
     4861 tggagctagg gagcgagcag gtctaccaga aggaactggt ccttatcgaa taatcaccca
     4921 gcttggtctc tatagctttg acgagcaaac caaaagagtt aaactcctgg ctcttcaccc
     4981 tggagttacg ctggatgatg ttagggccaa tacttctttc gagatcctca tccccgaaat
     5041 ggttcaggtc actcatccac ccacggaaga agagctccgg ttgcttcacg aaatggatcc
     5101 tattggtgta atagttcgca agtagagaca gatatatgtt tcaggcgccc ctctcgtcta
     5161 tcagccccga tgattaccca taggtcgaga tgtcgaagat ctgaccgcac ttaggtcagt
     5221 cgaagatctg accgctcttg ggtcagtcga agatctgatc gctcttgggt cagacgaaga
     5281 tctgaccgca cttaggtcag acaaatagac tccttcaggt acaaccattt ataatagccc
     5341 ccatagtgga cgaagtcgga aaacgttgac tttgccctag gtatttgcaa caacaataag
     5401 cgggaaggcc taccaacttg ctggagaagt tggcgcgatg ctagccttag gctggccagg
     5461 tggctcagtc tggtgctgtt acgaacatag gaatcaaaca tgtatttacc aaaacaattt
     5521 gaagaaacgc aatttcacgt aatgttcgaa ctaatccgcg ctcatccact ggctaccttg
     5581 gtgacattgt cttcgggtgg cttgaacgca aatcacatcc ctttgtccct atcggaatcg
     5641 ccccctccgt atggaacttt gcgtggtcat attgctcgtg caaacccatt atggagcgat
     5701 ttgaatagtg atattgagtc cttggcggtg ttcagcggcc ccgacactta tatttctcca
     5761 tcttggtatg cgacaaagaa ggaaactggc aaggttgtac ccacctggaa ctacgcagtt
     5821 gtacacgctt atggcatttt acgtattgta gacgacgctt cctggttgcg ctctcaatta
     5881 gaagcgctta ccatccacaa tgaagcatca ttctctgagc cttgggcggt ttccgatgct
     5941 cctcaagatt tcactgaaag actcatcagc tctattgttg gtgttgaaat gatcatcaca
     6001 cgcttatctg gtaagtggaa ggtgagtcaa aaccagccac cccaaaatca ggctagcgta
     6061 gtagcaagtt taaaggcaag tgatatacca gatgcgccat caatggctac attggtagaa
     6121 accagatcga aaaacgctcg ctaacgcagc atgcagccca ttgggcggtc taggttgtat
     6181 tcgggtagta tgaagaagaa catgagaact gatcaaacag caaccacaaa catcgatgaa
     6241 tacatggcgg gcttccctaa tgaagttcga gagattttgg aaaagataag gatgactata
     6301 agggaagttg caccagatgc tgatgagacg atcaagtatg gaattcccac ctttacactt
     6361 aagggtaatt tagtgcattt tgctgcctat aaaaagcata ttgggtttta tcctacccca
     6421 tcaggaattg aggaatttaa aattgagctg tctgcctatg agggtgctaa ggggtcggtg
     6481 aaatttccga tagataaaac gatcccctat gaattaatca gaaaaatcgt agcctttagg
     6541 gtaaaagaaa acctggaaag agcaaaagca aaaggaaaga aggaaaaata aaaactggct
     6601 accacaatag ctatgatgct gaattcaaat cactacctaa tatagggtgt atccgatgga
     6661 tgacatgccc ccagacaacc tctggtgctg tggagtatag attcaagcga acatgcccct
     6721 ggtcgtagtg ttcggaggct tcctgtttag cgagcgcagg gggaaatcaa tgacaaccac
     6781 aaggtggaag cttttatccc agattgaatc tggaatgact agctgttata agcagtttga
     6841 tcttactcga gtgtggtcct gaggcgattg acgtgccgta cagagaattt cgcgcgataa
     6901 tataaaagtg acgttctcgt gaaatcaggc atattttcgt tccgggtggg attcaccacc
     6961 tgattcaaag gcacgatctc ttgatttcat aagaaacagg ttcagccatt tgacacaatt
     7021 ctagccgggc tgcaaagcct gctcttctgg agataaagaa ggctaatcaa taatttcaga
     7081 gatttaccat gttttataaa aacctgccat atgtcgggaa tattttggct gcaatctcaa
     7141 gcgacagagt gcagtatttg gtctttattg gtttatcttt tgtgatctta attttcacgg
     7201 gggttttgta ttattcggac aaccagattt ttcagcgatt tatcggcaaa ataaacccca
     7261 ttctagcgat tttgctggcg ataattttag ggattattct attttctttt cttctttctc
     7321 aaaaatggtt tgcaattttt gggaaggaga ctctcgatgg agtaattcgc gcctctgtat
     7381 tggcggcagt atttggggta ataatgatct tggtggatat gaagatcgtt tttcctgccg
     7441 atacgaacat tttatttcct gaatccatac ttttttatcc ggcaattggt ttttttgttg
     7501 aaattctttt tcatctactc cccgtgactg tgttgttgtt gctattaaat tcaattttga
     7561 agaatgccaa tatcttttgg attagctttc taattgtggc tctgttagag cctgtctatc
     7621 aggctttgga tatggcgaat tcaagccgat atcctttgtg ggctgtgctt tacgttgggc
     7681 tgcatatata tctctttaat ctttttcaat tgctcatatt caaaaaatat gattttattt
     7741 cgatgtactc gtttcggctt gtgtattacc tgttttggca tattggatgg ggctatatca
     7801 ggttaaggtt gcttttctaa ataaagcacg ctaataatag atcagcgaaa ccctgttgat
     7861 cgtaccgata tgctataacg gtatataatg aaataaggct agctgcatct tactggtagg
     7921 cgacgatatc ctcctgcacc atagcctggc tttcatctgg aataaacaga ccccagataa
     7981 attcagctat ggcgtggtga gaagtattgg tgggttgtgg attttggatg tggctaccaa
     8041 tgtcggtggc catattattg tgcggttccc gatctaaatc ataggaagct gccatggata
     8101 agcaaacttc tcttttcaaa ggcaggatct ggcgctatcc gttcaattgg ctattggttg
     8161 cgattttctt gagcatcatg atctacctgg tatggggtgg attccaggta cgcgataaaa
     8221 aatcaaaccg cttgatcgtg tacgcttaca gtacccagga agaagttctg acccagggaa
     8281 ttttcccggc tttcgagcaa gcatgggaag cggaaactgg tgaggacata gccattgagg
     8341 gattattcgg cccttctggt acgctttcac tccagatcac cgagggggca cctgccgata
     8401 ttgctatttt cagcaatcag cggcatatcg actggctcaa gtttaacaaa agtgtgcgaa
     8461 gagataccga accaatcatg atcgcatcga ctccgttggt gatcattacg cgtccgggta
     8521 actcagcgaa tatagcagat ttcgctgatc tgaccctgcc tggtattaaa ttgcttcatg
     8581 cagatccggg gagttctggt gctggcgaat ggtcggtatt agcagagtat ggcagtgcct
     8641 atctggtaag cggagaccac gacagcgctg aagctcaagt caaaaatatc tggcaaaatg
     8701 tccggctggt gggatcgtct gcccgtgcta atatgacttt gttcgaactg ggcgcctgtg
     8761 atgccctagt gacttacgaa caggatgcgt atctggcgcg ggagcgcggt gttccgctcg
     8821 agatcgtttc tccgccacgc actatactgg caatgcatta tgctgtgatt gtggatgaaa
     8881 atgtcacttc gcatgagcgt cccgttgtgg aggcgttctt ggatttcatc cagagcgatc
     8941 aagggcagca aatattaagc cggtattacc tgcgggttac aaccattgaa agcgatgttt
     9001 tgcctgcgtt gattcaacct tttacggtgg aagatctcgg cggttggaca caggcccata
     9061 acacgttgat agaggattta tggatgaaag aaattaagcc gtaccttgca ttggaatctg
     9121 ccaatacttt gcttgggaca gcaaaatagc catgagatta tcattctcgt acttgctcaa
     9181 ttggattttg aaggggaaca tgcgatattt cacgtacgaa atgatcgcct tgagcataaa
     9241 actacgggac gtttatagct aagttatctg ggggtcaata ataccctttt ccgtttattg
     9301 ctaacagcag gctatggaac agtatctgca ggtctaacca gatactccaa ttgcaaatat
     9361 agtacatgtc caaacggatg cgctcgctgg ttgacaggtc tgagcggcca aagctctgcc
     9421 acaaaccggt cattcccgga cggacggaga gcaaattcat atcccagcgg gcgtacttgt
     9481 ccatctctac cggggttatt gggcgcgggc ctatcaacga gatatcagct ttcaacacgt
     9541 tgaaaatttt aggcaactca tccaggttgg tcctgagcag ccatttgccg acccgtgtca
     9601 ggcgcgggtt gtttcggtaa tattgataac tggttggctt cgcaggaagg cgtgggtacg
     9661 ccttcaggtt tacagcctca tcactatgtt ctgtgcggaa tttaaatgcc tcaaactgtt
     9721 tgccgttcaa gccaatgatg cgctggcgac tgaatatcgg tcctggcgag tccagcttta
     9781 tggcgatggc aattagcaaa ataaatggaa aaatgaaaat cgtgccaagg agggccaggc
     9841 tgaaatcgaa aaccagcttg agggctctat cgaccttggt cagccgtatg ggattgatcc
     9901 taaccagcgg cacaaaggca atatctttga cggttagccc ggtggtaata atttcataca
     9961 ggcctgagga aatgcgcaca ttcacatggt tggtgttgct ataacgcctg aagatctcca
    10021 gcaccttatc acgagatgag aaggcgctgg ttgctaggat aatctcttct acaccatatc
    10081 gttcgacaat tgcttcgagc tcatccactg atcccagaca gtagaggtgg tttatcacct
    10141 ttttaccggc tggcaatttt ttgtccagaa aacccaatat
//
LOCUS       DBNGSX010000272         2106 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1892072, whole genome shotgun sequence.
ACCESSION   DBNGSX010000272 DBNGSX010000000
VERSION     DBNGSX010000272.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1892072"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="AC16CB_08290"
     CDS             complement(<1..658)
                     /locus_tag="AC16CB_08290"
                     /inference="COORDINATES: protein motif:HMM:NF019561.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HIR3342507.1"
                     /translation="MWRWLGRNLSTLLLAFLLALVVWVSAVISTDPTVEADFPRPIEL
                     EVLGQDPAFVQVERIPPTVQLTLNAPQSIWANLNQDTIRAWIDLSGIEGGPHEIPVRF
                     QVDQSPVQVVKIDPPVVKVNLEPLVTHTYPITVNVTGDPALGYRRGAVQVTPSDVTVS
                     GPSSLAEQVVDVRVSLDVSNANDTVRNNITVQPVDQNGKPVAGLSVTPSTVELIQVVS
                     L"
     gene            complement(659..1543)
                     /gene="cdaA"
                     /locus_tag="AC16CB_08295"
     CDS             complement(659..1543)
                     /gene="cdaA"
                     /locus_tag="AC16CB_08295"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HIR3342508.1"
                     /translation="MPEFINRLLTNLYFIYERLDWVNAVDLLLVTMVFFAILLLLRDT
                     QAMVLLRGVLLLIVLISLLTSLELLPAFSWLVRSTLPALLLAIPVIFAPEIRRTLERL
                     GRAGAIIPGGKANSASQDAITSVVSAAARLSERKHGALIVMQRTDSLDEFVKTGVSLQ
                     ANVTPELLLQIFYPNTPLHDGAVIITGTDLVAASCVMPLSSSGVLAPTPERQMGLRHR
                     AALGISEVTDAVAVVVSEETGYISAVHGGRMIRRLDSERLENILRAFYRPMERRKALG
                     VILSGLFSRRSTSTREEN"
     gene            complement(1555..>2106)
                     /locus_tag="AC16CB_08300"
     CDS             complement(1555..>2106)
                     /locus_tag="AC16CB_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342509.1"
                     /translation="WQELFPAAETGSLRSVKAAEGELAVQRARPRQASEIASLITRLS
                     GGKRKLQRDDIMEAFGEKAYLLLSLGENPVGLAGWQVENLVARTDEVYLDPTIPYNQA
                     MQALTEEVERASRELQCEISLVFLPPELIQRNEVWSSLGYEERNIHSLQVRAWEEAAQ
                     ESMPPGSKMLFKQLRKDRVLRPV"
BASE COUNT          526 a          592 c          531 g          457 t
ORIGIN      
        1 ataaactcac cacctgaatc agctcaactg tcgacggagt tacacttaag cccgcaacag
       61 gtttgccatt ttggtcaacc ggttgtacag tgatattatt tctgacggta tcatttgcat
      121 tgcttacgtc caatgaaacc cttacatcca ccacttgttc tgccagagag ctcggaccag
      181 atacagtcac atcagaggga gtaacctgga ctgctcccct gcgatagccc aaggccggat
      241 ctccagtcac attgacggtg ataggatacg tgtgtgtgac cagcggctcc agattaacct
      301 tgactactgg cggatcaatc ttgaccacct gtactggaga ttggtccacc tggaacctga
      361 ccggaatctc atgtggccca ccctcaatac ccgaaaggtc aatccaggct cgtatagtat
      421 cttgatttaa atttgcccaa atggattgag gggcatttag tgtcagttgg acagtgggtg
      481 gaatcctctc aacctgcaca aaagccgggt cctgacctaa aacctctagc tcgatggggc
      541 gcggaaagtc agcctctacg gtcggatcgg ttgagataac cgcagagacc caaaccacca
      601 gcgccaacaa aaatgccagc agcagggtag ataggttcct tcccagccaa cgccacatct
      661 agttctcctc ccgggtcgag gtagatcgac gagaaaataa tccagacaat atgacaccca
      721 aagccttgcg tctttccata ggacggtaga aggcccgcaa gatgttctcg agacgctctg
      781 agtcaagcct gcgaatcatg cgaccaccat gcactgcaga gatatatccg gtctcctctg
      841 agacgaccac agccaccgca tcggtaacct cggagatacc cagtgcagct cgatgtcgca
      901 ggcccatctg gcgctcaggg gtcggcgcaa gcacgccgct ggaagagagc ggcatcacac
      961 aggatgcggc taccaaatct gtgccagtga tgatcaccgc gccatcgtgc aagggagtgt
     1021 tggggtagaa aatttgcaac aacaactcag gggtaacatt tgcttgcaag ctcactccgg
     1081 ttttcacgaa ttcatccagg ctgtcggtgc gctgcatcac aatcaatgcc ccatgtttgc
     1141 gctcggagag gcgcgcagct gcgctaacca ccgaggtgat ggcatcctga gaggccgaat
     1201 ttgccttccc acccggaatg atcgcgccag ctctgcctaa tcgctccagt gtgcggcgga
     1261 tttcaggtgc gaagatgacg ggaatcgcta gaagaagtgc tggcagtgtg ctgcgcacca
     1321 gccatgagaa agcaggcagc agttccaggc tggtcagcag gctgatcagc acaatcagaa
     1381 gcaagacacc ccgcagtaac accatagcct gggtatcgcg cagcaggagc agtatggcaa
     1441 aaaacaccat ggtgaccagc aataggtcaa ctgcgttcac ccagtccaaa cgctcgtaga
     1501 taaaatataa gttcgtaagt aggcggttga tgaattcagg cacagccaga acaactaaac
     1561 cgggcgcaaa acccgatctt tccgcaattg tttaaataac atttttgatc caggtggcat
     1621 tgattcttgt gccgcctcct cccaagctcg cacctgcaaa ctgtggatgt ttcgctcttc
     1681 gtaaccgagg gagctccaca cttcattccg ctgtattaat tccggtggca gaaataccag
     1741 ggaaatttcg cattgcagct cccgggaggc gcgctctact tcttcagtca atgcctgcat
     1801 ggcctggtta tatggaatgg tggggtcgag atagacctca tcagtgcgcg ccacaagatt
     1861 ttctacctgc caccctgcca gccccacagg attctctccc aagctaagga gcaaataagc
     1921 cttctctcca aatgcctcca tgatgtcgtc acgctgcaat ttgcgcttgc ctccgctaag
     1981 gcgggtgatc agagaggcaa tttcactagc ctgccgtggt cgtgcgcgtt gtaccgctag
     2041 ctcaccttcg gctgccttaa ccgagcgcaa agatccagtt tcggcggctg ggaaaagttc
     2101 ttgcca
//
LOCUS       DBNGSX010000273         4329 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1061368, whole genome shotgun sequence.
ACCESSION   DBNGSX010000273 DBNGSX010000000
VERSION     DBNGSX010000273.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1061368"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..1251
                     /locus_tag="AC16CB_08305"
     CDS             259..1251
                     /locus_tag="AC16CB_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR3342510.1"
                     /translation="MRLFEQGNVDDRSLKMVELKALAPGAGQISLKVQACGVCHTDLH
                     TVEGDIHPPRLPVTPGHQVVGIVEAVGAEVGESFKVGDRVGVPWLFSACRSCVFCARG
                     QENLCPNARFTGFHVDGGYAESMIAEAAHALPLPTAINPQQAAPLLCAGIVGYRSLRL
                     SDVQPGETLGLIGFGASAHLVIQVALHWGCTVYVFTRSYEHRRHALELGAAWAGGIED
                     QAPRMLDRAVLFAPAGYLVPLILEKLRPGGTLAINAIHTSPIPEMPYHLIYGERTLRS
                     VANATYKDGVDFLQLAAEIPVRSTVTAYSLDEANRALSDMKHSRINGEAVLIIS"
     gene            1332..2087
                     /locus_tag="AC16CB_08310"
     CDS             1332..2087
                     /locus_tag="AC16CB_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014027655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR3342511.1"
                     /translation="MSGHSHWATIRRKKGAADAKRGQIFTRLAREIVIAAREGGGDPS
                     TNIRLQYAIDRARAQNMPKENIERAIKRGTGESKEGNAIEEVTYEGYGPHGIALMISC
                     VTDNRNRAVAEIRHILNRMGGTMAEAGSVAWQFTRKSYFSFPSEGHDTNTIFDLVVEA
                     GADDVTFEDETVEIFGPVELFKEISDHLKSANIQPEEAELRMFPNNEVELSPDESMQV
                     YKVIDALEELDDVADVFSTLSISDEVMAQLEAA"
     gene            2107..2604
                     /gene="ruvC"
                     /locus_tag="AC16CB_08315"
     CDS             2107..2604
                     /gene="ruvC"
                     /locus_tag="AC16CB_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIR3342512.1"
                     /translation="MLVIGVDPGTAITGYGLVRENADGSLISVDYGVIQTSSDLDMPQ
                     RLLELHRVLNEVLLLHRPESGAVEKLFFQRNVRTALSVGQARGVALLALAEAEVSIAE
                     YTPLEVKQAVAGYGGADKNQVQQMVRVLLGLEDVPRPDDAADALAVAICHLHSLKMKG
                     IMGLS"
     gene            2601..3176
                     /gene="ruvA"
                     /locus_tag="AC16CB_08320"
     CDS             2601..3176
                     /gene="ruvA"
                     /locus_tag="AC16CB_08320"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR3342513.1"
                     /translation="MISSISGRVQEVNSDNVVIEVGGVGLLVFVPATTRDHLQPGASV
                     FLHAHLVVREDSLSLYGFDSHEGRDFFLLLLGVNGVGPKLALSVLSTLTPDAIRRAVF
                     HEQDEVFARVPGVGKKTAQRILLHLQDRVRMVAGLEPVAGMTDVDTEVMNALTALGYS
                     VVEAQAALQSIPRDAPEELEDRLRLALQYFS"
     gene            3196..4050
                     /gene="proC"
                     /locus_tag="AC16CB_08325"
     CDS             3196..4050
                     /gene="proC"
                     /locus_tag="AC16CB_08325"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIR3342514.1"
                     /translation="MLENQKIAFIGPGVMAEAMISGLIRRNFAQPKMLTAAGPTIERL
                     SHLATKYGIFTDTDNAQAVRNADVVVLSVKPQRLDKVMIGMHGHIKPSALVLSIIAGA
                     SIDKISHGLGHEIVVRSMPNTPAQIGEGITVWTASSQVSERQREVAQQILQALGEEIF
                     VEDEIYLDMATALSGTGPAYVFLFMEAMVDAGVHLGFPRRIAELLVAQTVRGSVDFYR
                     NHEDAIHFARLRNQVTSPGGTSAAALYYLEKAGFRTAISRAIWAAYERSRELGRSSKP
                     HAPEGESS"
BASE COUNT          996 a         1049 c         1257 g         1027 t
ORIGIN      
        1 attataaccg aggcaggggg ctttgaccag ggttggttcg gacggtcttc tctagaattg
       61 tatcgccagc ctcggtataa tggatttgtc acaacatgat gcgttatagt tgctttatta
      121 gcaatggcaa atgccataag gaagatggat gccagagaaa agggaaactt tcgtatagcc
      181 ataaagggac tgataacagg atgatttgaa tccgacatct gagtaaatat tgggattgtg
      241 aggcgagtga tgcgagcaat gaggcttttt gagcagggaa atgttgatga tcgcagtctg
      301 aagatggtcg aattgaaggc cttggccccc ggagcagggc aaattagctt gaaggtgcaa
      361 gcctgcggcg tgtgccatac cgatctgcac acggtagaag gcgatatcca cccgccgcgc
      421 ttgcccgtca ctcctgggca ccaggtagtt gggattgtgg aggctgtcgg ggcggaggtt
      481 ggcgagagtt tcaaggttgg cgatcgggta ggtgtgccct ggctgttcag cgcctgccgc
      541 tcatgcgtgt tctgcgcccg cgggcaggaa aacttatgcc ccaatgctcg ttttaccggt
      601 tttcatgtcg atggaggtta cgcagagagc atgattgccg aggcagctca tgctctgccc
      661 ctgcccacgg cgatcaaccc ccagcaggct gcgccattat tgtgcgcggg cattgtagga
      721 taccgctcgc tgcgcctttc cgatgtgcag cccggtgaga cccttggcct gataggattt
      781 ggagccagcg cgcatctggt tatccaggtg gcgctccact ggggctgcac ggtgtacgtc
      841 ttcacgcgct cgtacgaaca ccgccggcac gccctggagc ttggggcagc ctgggcaggt
      901 ggcattgaag atcaagctcc tcgtatgttg gatcgcgccg tgctctttgc gccggctggg
      961 tatctcgtgc ctcttatact agaaaagctg cgtccaggag ggacgctggc gattaacgcc
     1021 atccacacct cacccatccc agagatgccc taccatttga tctatgggga gcgcaccctg
     1081 cggagtgtcg ccaacgccac gtacaaggat ggcgtggatt ttctgcaatt agccgctgaa
     1141 atccctgttc gctccacggt caccgcgtac tcgctggacg aggccaaccg agctctctca
     1201 gatatgaagc acagccgcat aaacggagag gcggtgttga tcataagttg atcggaatgg
     1261 tataatctac agggtagtgt acgatcccca agttgataat ttgcgttatt tcatttgcag
     1321 gagaggttta tatgtctgga cattcccatt gggcaactat ccggcgcaag aagggtgctg
     1381 cagacgccaa acgaggccag atttttaccc ggctggcacg cgagatcgtg atcgcggcgc
     1441 gtgagggcgg cggcgaccct agcacgaata tacgtttgca gtacgctatc gaccgtgccc
     1501 gagcgcaaaa catgcccaag gagaacatcg aacgtgccat aaagcgcggc acgggagaat
     1561 caaaagaagg caacgcaatt gaggaagtga cgtatgaagg gtatggcccg catgggattg
     1621 ccctgatgat ctcttgcgtt accgataatc gaaaccgggc tgtagctgaa attcgccata
     1681 tccttaaccg catgggcggc acaatggcag aggctggctc ggtcgcctgg cagttcactc
     1741 gcaagtcgta tttctctttt ccgtccgagg ggcatgacac gaatacgatc ttcgatctgg
     1801 tagttgaagc cggcgccgat gatgtgacct ttgaagacga aaccgttgaa atttttggac
     1861 cggtagagct atttaaagag attagcgatc atttgaaatc ggccaacatc cagccagaag
     1921 aggcggaatt gagaatgttc cccaataatg aggttgagct gagcccagac gaatcgatgc
     1981 aggtctataa agtgattgat gcattggaag agctggatga cgtggcggat gtcttctcaa
     2041 ccttaagcat ctccgacgag gtgatggccc aattggaggc cgcctaacgg tggattgcca
     2101 gggagcatgc tggttattgg cgtagaccct ggaacagcga tcaccggata tggattggtg
     2161 cgggaaaacg ccgatggcag cttgatatcc gtggattatg gcgtgattca aacctcctca
     2221 gatttggaca tgccacagcg tttgttggag ctacaccggg ttttaaatga ggtcttactt
     2281 ctccatcggc ctgagagcgg cgcggtggag aagctgtttt tccagcgtaa tgtgcgcaca
     2341 gccctgagcg tggggcaagc gcgcggtgtg gcgctcctgg cgctggcaga ggctgaagtg
     2401 tcaattgcgg aatacactcc cttggaggtt aagcaagccg tggctggata cggcggcgcg
     2461 gataagaacc aggtgcagca gatggtgcgc gtccttttgg gattagaaga tgtgcctcga
     2521 ccagatgacg ccgcagatgc cctggcagtg gcgatctgtc acctgcactc tttgaaaatg
     2581 aaaggcatta tggggttgtc atgatttcca gtatcagtgg acgtgtgcaa gaggtcaatt
     2641 cggataatgt ggtgattgaa gtgggcggtg ttggcttgct ggtttttgtc ccagcgacca
     2701 cgcgggacca cctgcaaccc ggggcgtccg tgttcctgca tgcgcatctg gttgtgcgcg
     2761 aggattccct gagtttgtat ggttttgatt cgcatgaagg gcgagatttt ttccttctat
     2821 tattgggtgt caatggcgtt gggcccaaat tggcgctctc cgtgctctcc acgctgaccc
     2881 cagacgccat tcgccgtgcg gtttttcatg aacaggatga ggtttttgcc cgtgtgccgg
     2941 gagtgggaaa gaaaaccgcg cagagaatac tgctgcactt gcaagaccgc gtgcgcatgg
     3001 tggcgggctt ggagcctgtt gcaggaatga cagatgtgga cactgaggtg atgaatgccc
     3061 taactgcact ggggtatagc gttgtggaag cccaggcagc cctgcaatcg atcccacgcg
     3121 atgccccgga ggagctcgaa gaccgtttga gattagcgtt gcagtatttt tcgtaagagt
     3181 tcgtggaggt tacagttgct agaaaaccaa aagatcgcat ttatcggccc gggagtaatg
     3241 gcagaggcca tgatctctgg attgatccgc cgaaattttg cccaacccaa gatgttaaca
     3301 gccgccgggc cgactattga gcgtttatct cacctggcaa ctaaatacgg aatattcaca
     3361 gacactgata acgcacaagc cgttcgaaac gcagatgtgg tggtgctttc tgtaaaacca
     3421 cagcgcttag ataaagtgat gatcggtatg catggccata tcaagccctc ggcattagtg
     3481 ttatccatta tcgctggagc cagcatcgat aaaatcagcc atggtcttgg acatgaaatt
     3541 gttgttcgct caatgccaaa taccccggct cagattggcg aggggatcac cgtctggaca
     3601 gcatcaagcc aggtaagtga gcggcagcgt gaagttgcac aacaaatatt acaggcgctc
     3661 ggggaagaaa tctttgtaga ggacgagatt tacctggata tggctactgc gctctctggt
     3721 accggacctg cctatgtgtt cttgtttatg gaagctatgg tggatgctgg tgtgcattta
     3781 ggatttcccc gtcgcattgc agaactcctg gtagcgcaga cagtacgcgg ctcggtggat
     3841 ttctaccgca accacgagga tgcgattcac tttgccaggc tccgcaacca ggtaacctct
     3901 ccgggaggta cttcggcagc tgccttatat tacctggaga aagcgggttt ccgaaccgcc
     3961 atctcacgcg ctatctgggc tgcctatgag cgctcgcgag agcttggtcg ctcgagcaag
     4021 ccccacgctc ctgaaggcga aagtagttaa tttagtagca gatacgagcg agtatttcac
     4081 tcacaaaact ttcgttcatc tttaccatca agagctcaat ctagccaggt ttcacgcaga
     4141 ttctcgacac cggcaaattg cgtgaaccaa gaaaattccg atctggtatt accttttaga
     4201 tacatgcctc gcgcatgccg atagcgccac ttacatcatc agctgccgaa taattcaata
     4261 tattcaaggg tgggtggaat gataaaaaca ctcttacttt tcctcatctt gatcctaacg
     4321 attggagct
//
LOCUS       DBNGSX010000274         5301 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1822658, whole genome shotgun sequence.
ACCESSION   DBNGSX010000274 DBNGSX010000000
VERSION     DBNGSX010000274.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1822658"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="AC16CB_08330"
     CDS             complement(<1..1212)
                     /locus_tag="AC16CB_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR3342515.1"
                     /translation="MSYEDAIPYVSPVWTRYTPIIAERAEGCYIYDQDGSAYLDFTSG
                     IGVTNTGHCHPRIVEAIRKQAGLLLHGQVNIVYHKPLLELIEELRKITPPSIDGFFFS
                     NSGAEAIEGAMKLARQATGKPNIIVFQGSFHGRTVGTMSLTTSKTIYRAGYQPLMPGV
                     FVAPFPYAYRYGWDEEKTSQWCLEELEYLLVSQTAPEETAALLIEGVLGEGGYVVPPV
                     SFMRGLREICDRNGILLIIDEVQSGFGRTGSWFSFEHYGITPDIIVVAKGLASGMPLS
                     GVFSSLDLMKKWVPGTHGGTYGGNAVAVSAAVATIQAMRDEDMLGNARSRGMQLMTGL
                     RHLQEEFPVIGDVRGMGLMIGTEFRSPNRKPDKATAKAVIHACQDRNLLLLSCGPWDN
                     TIRWIPPLMVNQ"
     gene            complement(1313..3223)
                     /locus_tag="AC16CB_08335"
     CDS             complement(1313..3223)
                     /locus_tag="AC16CB_08335"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HIR3342516.1"
                     /translation="MAYGYNGKILHVDLTHGTLEIEEPPESFYRKYMGGSAMGLYYIL
                     RDMPAGVDPLSPDNILTLMASVTTGAAISGQSRINANAKSPMGGAIGDSQSGGFFPAE
                     LKFAGFDGIVVKGRSEKPVYLSILFGEVALHDASHLVGKVTGEVDALLRDEVNEPKAE
                     VLQYGPAAENGVRFSSLVSMANRNNGRTGMGLVMASKNLKAIVVRGKAKVQIADPKKL
                     VELNRSGPKLLPENPDVDALFQHGTANVLMPQNTTGTLPTFNYNEGQFEFCEDISGER
                     MTETILKDRDTCYACVVRCKRVVEITEGAYKADPLYGGAEYETLSTFGSYCGVKDLEA
                     ISLANQICNMYGVDTISCGATIAFAMECYEKGIIGSSETGGIELRFGNTQAMLDTLQQ
                     IVENTGPLGKVLSQGSERAAKVWGPAAEDCLITVKGEEAPAHMPQAKKSLALIYAVNP
                     FGADHQSSEHDPMYEEDCLPLYLERLAELDLKDVPPYGSLGREKVRFATYTHIFYSLL
                     DTLELCQFVWGPAWTLYGPSEIVEFVRAVTGWNVSLFELVKVGQRRLNMLRAFNAREG
                     FSRKDDKLPKKFYKALQGTGPTAGVAIDPAEFEAALDMYYQLLAWTPDGTPTRTALAD
                     LGIEWVADTVGV"
     gene            complement(3287..4441)
                     /locus_tag="AC16CB_08340"
     CDS             complement(3287..4441)
                     /locus_tag="AC16CB_08340"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3342517.1"
                     /translation="MKKTMLWRLLAAMMVLSLLLAACGGGGGAPSAGEAEEFVTGSGF
                     VCPQPNPRVEVTSKELNLFVWTEYIPVEWKECFELVYGIKVNHDEYSSNEEMYAKLSA
                     GATTYDLILPTDYIVSLMVGQGMLQEWDHAKLPMLENIDSGYKDLPFDPGNKYTIPYM
                     AGTDALVYSVERVATPPTSWADLWNPDYAGRLVLLDDPRVVIGFTLLTLGYDLNTKDP
                     AQLEEAKTKLLELIPNVKLFDSDSPKTALIAGDVDVGNVWNGEAFTAQQEDPNFEYVF
                     PSEGTILWQDNWAIPTSAPHLDAVYAWVNYIEQGDMFWMMLRDFPYTVPNAAALEYAK
                     ENETELYDAYINSPITNTPPEVLANGFFMEDVGEATPLYDQIWTEVKGEE"
     gene            complement(4561..>5301)
                     /locus_tag="AC16CB_08345"
     CDS             complement(4561..>5301)
                     /locus_tag="AC16CB_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3342518.1"
                     /translation="QVGPTEIDSGNVIQSPCGAFHWFCELAKNIEVRNAARNTLTIAF
                     VSTIISTLIGTLAAIALQRYNFKLKTFSEVSLYIPIVIPEIVMGIGILVLFSQMFTLL
                     NSALSLSGDSQLTLGLATVIVSHIAFSIPFVTLVVRARLHGFDKSIEEASMDLGANEI
                     ITFWRVTLPSIMPGVLSGALLAFTLSLDDFVITFFTTGPGASTLPIYVYGLLRRIVTP
                     QVNALSAVWISIVLVAVFFTQRLQARAD"
BASE COUNT         1305 a         1461 c         1303 g         1232 t
ORIGIN      
        1 ttgattcacc atcaacgggg gtatccatcg tattgtgtta tcccacggac cgcatgaaag
       61 caaaagcaag ttacggtcct ggcaagcatg gataaccgct ttcgctgtcg ccttatctgg
      121 ctttcgattt ggggagcgaa attcagtgcc aatcattaac cccatcccgc gtacatcacc
      181 gatgaccggg aactcttctt gtaaatggcg caagccggtc atcaactgca taccgcgcga
      241 gcgggcattg ccgagcatat cttcgtcacg cattgcctga atagtagcca cagccgcaga
      301 tacagcgaca gcatttccac cgtacgtacc gccgtgtgta cccggcaccc attttttcat
      361 taggtccagt gagctgaata cacccgaaag cggcatacct gaggcaagcc ctttggctac
      421 cactatgata tcgggagtga tgccataatg ttcaaatgag aaccaggagc cggtgcgccc
      481 aaatcctgat tgaacttcgt cgattatgag caagattcca ttccggtcgc aaatttcacg
      541 caaaccacgc ataaagctaa ctggcggcac tacatatcca ccttcaccca gcactccttc
      601 aataagcaga gcagcagtct cctcaggtgc tgtttgggat accaggagat attcgagctc
      661 ctccaggcac cactggctgg tcttctcttc atcccagcca tagcggtacg catatggaaa
      721 cggagcgaca aaaacacccg gcatgagtgg ctggtaacct gcacggtaga tggtctttga
      781 ggtggtcaat gacatggttc caaccgtgcg cccatgaaaa ctaccctgga atacgatgat
      841 attaggtttg ccggtcgcct ggcgcgccag cttcatcgcc ccttcaatcg cctctgcacc
      901 tgagttacta aagaagaatc catcaatgct tggaggtgtt atcttgcgca gctcttcgat
      961 caactccaac agtggcttgt gatatacgat atttacctga ccatgcagca gcaagccagc
     1021 ttgtttgcga atggcttcga ctattcgcgg atggcagtga ccagtattgg tcacgccgat
     1081 accagatgta aagtccaggt aagcggaccc gtcctggtcg tagatataac acccttcagc
     1141 acgctcagcg atgatgggtg tatatctcgt ccaaacaggg gaaacataag gaattgcgtc
     1201 ttcgtagctc ataagactat gctctttttt atctaaatga atgataggca tgagtgtaga
     1261 gagcgcatta tatcaggtaa tgctctctac actcattctt gagttgtatc tactaaacac
     1321 ctactgtatc agccacccac tcaataccca aatctgccag ggcagtccgg gtgggggtgc
     1381 catccggagt ccaagctaag agttggtagt acatgtcgag ggcagcttca aactccgcag
     1441 gatcaatcgc cacgccagcc gttggaccgg ttccttgaag cgccttataa aacttcttgg
     1501 gcagcttgtc atctttcctg ctaaagcctt cgcgggcatt gaacgcacgc agcatgttca
     1561 agcggcgctg accaactttc accaattcga acaaactgac attccaacca gtcaccgcgc
     1621 gcacgaactc aactatttct gaaggcccat aaagcgtcca tgcaggtccc caaacgaatt
     1681 ggcacagctc caaggtatcc agcaggctgt agaagatgtg ggtgtaagtg gcgaagcgta
     1741 ccttttccct tcctagcgat ccatagggag gcacatcctt caaatccagc tctgccaatc
     1801 gctcaaggta aagaggcaaa caatcttcct cgtacatagg gtcgtgctcg ctggactggt
     1861 gatctgcacc gaaggggttg acagcgtaga tcaaggcaag cgatttctta gcctgaggca
     1921 tatgggctgg ggcttcctcg cctttgactg taatcaaaca atcttcagcc gctggacccc
     1981 acacctttgc tgcccgctca gaaccctgcg agagcacctt gccaagtgga ccagtgtttt
     2041 caactatttg ctggagcgta tccagcatag cctgggtgtt gccaaaccgc aattcgatac
     2101 caccagtctc acttgatccg attatgcctt tctcatagca ctccatggcg aaagcaatgg
     2161 tcgcaccgca tgagatcgtg tccacgccgt acatattgca gatttggtta gctaaagaaa
     2221 ttgcctccaa atccttaaca ccacaatacg agccgaacgt ggatagggtt tcatactccg
     2281 cgccaccata aaggggatct gctttgtaag ctccctcagt gatctccacc acgcgtttgc
     2341 agcgcaccac acatgcatag caggtatcac gatccttaag gattgtctcg gtcatgcgct
     2401 cgccgcttat atcttcgcag aactcaaatt gaccttcgtt gtagttgaag gttggcaacg
     2461 tgcccgtcgt attttgtggc atcagcacat tagcagtgcc atgctggaag agagcatcca
     2521 catcgggatt ttcaggcaat aactttggcc cactgcggtt gagttccacc agctttttgg
     2581 gatcggcgat ttgtaccttg gctttgcctc gcaccacaat ggccttgagg tttttactcg
     2641 ccatcaccag tcccatacct gttcgcccat tgttgcgatt tgccatgctc accaggctgg
     2701 agaagcgcac gccgttttct gcggcaggcc cgtattgcaa aacttctgcc ttgggctcat
     2761 tcacttcatc cctaaggagc gcatctacct caccggtcac cttaccaacc aaatgggaag
     2821 catcgtgaag agctacctca ccaaataaaa tgctgagata aaccggtttc tctgagcgtc
     2881 ctttgacgac tatgccatca aaaccagcaa acttgagctc cgcggggaaa aaaccacccg
     2941 attgcgaatc gccgatcgct ccgcccatag gagattttgc gttcgcattg atacggctct
     3001 gcccagaaat agccgctccc gtcgtcacac tagccattaa agtgagtatg ttgtccggcg
     3061 atagaggatc gacacctgcg ggcatatctc tcaagatgta atatagcccc atcgcactgc
     3121 cgcccatata cttccggtaa aagctctcag ggggctcttc tatctccagc gtgccgtgag
     3181 ttaaatcaac atgcaggatc tttccattgt atccataagc catagcaacc tcctttgcaa
     3241 tagaaacccg ttttcttgga gaaaacgggt ttctcacgat tgggaattac tcctcgccct
     3301 taacctccgt ccaaatttgg tcgtagagcg gggtggcctc gccgacatct tccatgaaga
     3361 agccattggc cagtacttcg ggaggcgtgt tggtgatcgg cgagttgata taggcatcgt
     3421 acaactcagt ctcgttctct ttggcgtatt ccagagcggc agcattgggc acagtgtagg
     3481 ggaagtcgcg caacatcatc cagaacatat caccctgctc aatatagttt acccaggcat
     3541 agacggcatc caggtgcggg gcgctggttg ggatggccca gttatcctgc cacaagatag
     3601 tgccttcgct ggggaagaca tactcaaaat tggggtcttc ctgctgggca gtgaatgctt
     3661 cgccgttcca gacattgccc acatccacgt ctccggcgat gagagccgtc ttggggctgt
     3721 cgctgtcgaa gagcttgaca ttggggatca gttccaacag tttcgtttta gcctcttcca
     3781 attgagccgg atctttggtg tttagatcgt agcccagggt cagcaacgtg aagccgatca
     3841 ccacgcgggg gtcatcgagc aaaaccaagc gaccagcata gtcgggattc cacaaatcag
     3901 cccaggatgt tggcggggta gcgacgcgct ccacactgta gacgagggca tccgtgccgg
     3961 ccatgtaggg gattgtgtac ttattgccgg gatcgaaggg caggtctttg tagccggaat
     4021 caatattctc cagcatcggc agtttggcat gatcccactc ttggagcatc ccctggccga
     4081 ccatcaacga gacaatgtag tcggtaggca gaatcagatc gtaggttgta gctccagcag
     4141 acagcttggc gtacatctcc tcgttgctgg aatactcgtc gtggttcact ttgatgccgt
     4201 aaaccaactc gaaacattcc ttccactcca ccgggatgta ctccgtccac acaaagagat
     4261 tcaactcctt cgaggtgacc tcgacgcgcg gattcggctg agggcaaacg aaacccgagc
     4321 cggtgacaaa ttcctcggcc tcgcctgcgc taggtgctcc accaccaccg ccgcaggctg
     4381 ccagcagcaa gctgagtacc atcatcgctg ccaacaatct ccataacatt gtttttttca
     4441 tttctgcctc ctgttaagat atatggattg aaagtagagc ggttttcagg aaaccgcctt
     4501 cgccggcagg tatccatacc tgccatatgc ccgtaagacc ataatagcta aaacagattg
     4561 tcaatctgcc ctcgcttgta agcgctgcgt aaaaaagacg gcgaccagca caatcgaaat
     4621 ccacaccgcc gagagggcgt tgacctgcgg agtgacaatg cggcgcagca atccgtagac
     4681 atagatcggc agcgtagagg cgcccggccc ggtggtaaaa aaggtaatca caaaatcgtc
     4741 cagcgagagg gtgaaggcta gcagcgctcc ggagagcacg ccgggcatga tcgagggcag
     4801 ggtcacgcgc cagaaagtga tgatttcgtt ggcgcccaaa tccatcgaag cctcctcgat
     4861 cgatttatca aaaccatgca accgcgctcg caccaccagc gtcacgaagg ggatgctgaa
     4921 agcgatatgc gagacgataa cggtagccaa tcccagggta agctggctgt caccactcag
     4981 gctgagggca ctattcaaca gggtaaacat ctgactgaat aagaccaaaa tgccgatgcc
     5041 catcacaatt tctgggatga caatggggat atatagcgaa acctccgaga atgtctttaa
     5101 tttaaaattg tagcgctgta aggcaatcgc cgccaaagta ccgattagcg tcgatataat
     5161 cgtcgacaca aaggcgatgg tcagtgtgtt gcgggcagcg ttgcggacct cgatattttt
     5221 cgccagttcg cagaaccagt gaaacgcgcc gcaaggactc tggatgacat ttccagagtc
     5281 gatctcggtc ggcccgacct g
//
LOCUS       DBNGSX010000275         1878 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_576297, whole genome shotgun sequence.
ACCESSION   DBNGSX010000275 DBNGSX010000000
VERSION     DBNGSX010000275.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_576297"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="AC16CB_08350"
     CDS             complement(<1..440)
                     /locus_tag="AC16CB_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342519.1"
                     /translation="MRKNLFTLLSLVILASMLLAACAPTAAPERIVETVEVVLTQIVE
                     IAGTAMVVTATPEPEPSGEPLKAVSPEFKNADTFMVIDGAGEQETLDPAWTYETRGSG
                     IEANIYEGMVWFNREKTDEFIPALATEWSASDDGLEWVFTIRQGV"
     gene            complement(613..1590)
                     /locus_tag="AC16CB_08355"
     CDS             complement(613..1590)
                     /locus_tag="AC16CB_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008179730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3342520.1"
                     /translation="MPEDQVILKVDKLVKHFPIYRGVFQRQVGAVHAVDSISFDVRRG
                     ETFGLVGESGCGKTTAGRTIIQLYKPTSGHIYYNGTDLTTLKGGDMRRMRKSIQMIFQ
                     DPYASLNPRISIADTLSEPILIHGLARGAQARQRVDELLNIVQLNPGFANRFPHEFSG
                     GQRQRIGIARALALNPELIICDEPISALDVSIQAQIINLLEDLQKQLGLTYLFIAHDL
                     SVVRHISSRVAVMYLGIIAELATVKELYNNPLHPYTQALLSAVPVPNPIIEEKRQRII
                     LEGDVPSPVNPPSGCRFRTRCPIAKELCAEQVPEWREVSPGHWVACHFV"
     gene            complement(1583..>1878)
                     /locus_tag="AC16CB_08360"
     CDS             complement(1583..>1878)
                     /locus_tag="AC16CB_08360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR3342521.1"
                     /translation="IIEEAPVKELYANPTHPYSIGLLGSLPSLTTDDRKRLTSIPGLP
                     PVLLEKPVGCPFAVRCDHAFDRCKQENPPLMSIGSLHRVACWWDVQEGRPRNA"
BASE COUNT          445 a          514 c          494 g          425 t
ORIGIN      
        1 acgccctggc ggatggtgaa gacccattcc aagccgtcgt cgctagcgct ccattccgtt
       61 gctaaagccg ggatgaactc gtcggtcttc tcgcggttga accacaccat gccttcgtag
      121 atgttagcct caatgccgga accgcgcgtc tcgtaggtcc aggcgggatc cagcgtctcc
      181 tgttcgccag cgccatcgat gaccatgaaa gtatcggcgt tcttgaactc cggcgagacc
      241 gctttaagcg gctctccgct gggttcaggc tccggcgtgg ccgtaaccac catggctgtt
      301 ccagcgatct cgacaatctg ggttaagacg acctcgactg tctcgacgat gcgctcgggg
      361 gccgccgtcg gcgcacaggc cgccagcaac atgcttgcca agatgaccag cgagagcaag
      421 gtaaataaat tcttacgcat actgaattct cctctcttta attgataacc attgggtttt
      481 gcttgtattt acatcaacac tgtgatctga aataaccttc aaccacctcc tttcgatgaa
      541 catatttttt tcactagcct gtcaaaacaa accacttata taaagggaga gtaaacatat
      601 agtcaatcta gatcatacaa aatgacatgc cacccaatgg ccaggtgaaa cctcccgcca
      661 ctcgggcact tgctcggcac acaattcttt tgcgatgggg caacgtgtgc ggaaacggca
      721 gcctgaaggc gggttcactg gactgggcac atcgccttcc aggataatac gctggcgctt
      781 ttcttcgatg atcgggttgg gcaccggcac cgcggaaagc aatgcctgtg tgtagggatg
      841 caatgggttg ttatacaact ccttcaccgt agcaagctcg gcgataattc ccagatacat
      901 gaccgccaca cggctgctga tgtggcgcac cacagacaga tcatgagcaa taaacaggta
      961 ggtgaggcct aactgtttct gtaaatcttc cagcaagtta ataatttgcg cctgaatgga
     1021 aacatccagg gcggagatcg gctcgtcaca gatgatcaat tcaggattta gagccagggc
     1081 acgcgcaatg ccaatgcgct ggcgctgtcc gccggagaat tcatgtggga agcggttggc
     1141 aaagccggga ttcaactgca cgatattcaa cagctcgtcg acgcgctggc gagcttgcgc
     1201 tccgcgtgct aatccgtgaa tgagtattgg ctcgctgagc gtatcagcaa tggatatgcg
     1261 aggattgagg ctggcatacg gatcctggaa gatcatctgt atgcttttgc gcatgcggcg
     1321 catatcgcca ccctttaggg tagtcaggtc cgtgccgttg taatagatat gccccgaggt
     1381 gggtttataa agctggatga tcgtacggcc tgcagtggtc ttgccgcagc ccgattcgcc
     1441 caccaggcca aaggtttccc cgcggcgcac atcaaaggag atactatcta cggcgtgcac
     1501 agcccctacc tggcgctgaa aaacaccccg atagatcggg aagtgcttga ccagcttatc
     1561 aactttcagt atgacttgat cttcaggcat tgcgcggcct cccttcctgg acatcccacc
     1621 aacacgcaac tcggtgcaat gatccaatgc tcattaaggg aggattttct tgcttgcaac
     1681 gatcgaaagc gtggtcacaa cgtaccgcaa agggacaacc caccggcttt tccaacagca
     1741 ccggaggcaa tccgggaata gaggtcaaac gcttgcgatc atcggtcgtc aggctgggta
     1801 agctgcccag caagccaatc gaataagggt gcgtggggtt ggcatacaac tctttgaccg
     1861 gcgcctcttc gataatgt
//
LOCUS       DBNGSX010000276         3221 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_22965, whole genome shotgun sequence.
ACCESSION   DBNGSX010000276 DBNGSX010000000
VERSION     DBNGSX010000276.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_22965"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..994)
                     /locus_tag="AC16CB_08365"
     CDS             complement(14..994)
                     /locus_tag="AC16CB_08365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3342522.1"
                     /translation="MEARLGGISRKPAIAEPTTIPVDFLHHRIVVVVPAYNEERFIGS
                     VVIKIRKYPVTVIVVDDGSTDDTAYVAQAAGAVVARHEKNQGKGQALNTGFCQARQLG
                     PEAIVIIDGDGQHLPEQLPRLVCPVLSGQADIIVGSRYIEKNSHVPRHRIWGHWAFNL
                     ITRFSSGIRTSDSQSGYRAFSPKAVDAIAFHSEGFSVESEIPFIAREHDLRLLEVPVT
                     VRYEDPPKRSVIAHGLAVLGGILRLVGQYRPLIFFGLPGATFLLTGLGWGLWIVERFM
                     QTKQLAIGNTFIFVLLFLTGQVMLSTGFILHSVRSLLHDMLLGRVKHNRG"
     gene            complement(1392..2354)
                     /locus_tag="AC16CB_08370"
     CDS             complement(1392..2354)
                     /locus_tag="AC16CB_08370"
                     /inference="COORDINATES: protein motif:HMM:NF013508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342523.1"
                     /translation="MKATSKFTKEILFLVALALTLALLAGLSGNGARPAAARPAFQEG
                     TSSPALTQEPPSPAVPQVTPSLTIQKIRNVELAYDADGDGNIDPGDKVSYTIRYQNTG
                     PEPVTGVTIEDAFSKELVANVNGTPMIDAVGATIEAEKIIWVVEDLPPGRDDQVDYVI
                     TIKGTLTPGPYTLNNAASILINGTPIAKSVVSEEVVVPTATPTPTSTPSPTPSPTVQP
                     TSTPPVTSVGVSNLQDPRILWPMVLATLAPVLGGLVVLGLIAIKPEILEQEERSRVIR
                     VGFVVTLTVGAVLVMGVFGGIERGAAAGILGTVAGYLLRGVKEG"
     gene            2763..>3221
                     /locus_tag="AC16CB_08375"
     CDS             2763..>3221
                     /locus_tag="AC16CB_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HIR3342524.1"
                     /translation="MGQRLALIIGNSAYLDGALSRLLTPDADVGALGEILLDPELGGF
                     DDVNVLVNMSSHIIRRSIASFFSKKAREDLLLLYFSGHGVLDDQGHLYLAVRDTDSKL
                     LRGTAISANYITDEMNNSRSQRQLLILDCCHSGAFARGTKGQPGASVGTAS"
BASE COUNT          709 a          900 c          919 g          693 t
ORIGIN      
        1 ccagccgatt gacctacccg cgattgtgct tcacgcgccc gagcagcatg tcgtgcaaca
       61 ggctgcgcac cgaatgcagg atgaagccgg tggatagcat gacctgcccg gtgagaaaga
      121 gcaggacgaa gatgaaggtg ttgccgatgg cgagctgttt cgtctgcata aagcgctcta
      181 ctatccatag gccccagccc agcccggtca atagaaacgt agcccccggc aggccgaaga
      241 atatcagcgg gcggtactgc cccaccagac gcaggatacc gcccaggacg gccaacccgt
      301 gggcgatcac ggagcgcttg ggcgggtctt cgtagcggac tgtgaccggc acttccagca
      361 agcgaagatc gtgttccctg gcaataaacg gtatttccga ttccaccgag aaaccttctg
      421 aatggaaagc gatagcatct acagctttgg gggagaaagc ccggtagccg ctttgcgaat
      481 cagatgttcg tatcccgcta gaaaagcgag taatcaagtt aaaagcccag tgtccccaga
      541 tgcggtggcg cggcacgtgg ctgtttttct cgatatatcg agagccgacg ataatatcag
      601 cctggcccga cagcacgggg cacaccaggc gggggagctg ctcgggcaaa tgctgcccgt
      661 cgccgtcgat gatgacgatg gcctctggcc ccagctggcg cgcctggcag aaacccgtgt
      721 tcaaagcctg gcccttgcct tgatttttct catggcgcgc caccaccgca ccggcggcct
      781 gggccacgta ggcggtgtcg tcggtcgatc cgtcgtcgac cacgatcacg gtgacaggat
      841 atttgcggat tttgattacc acgctgccga tgaagcgctc ttcgttgtaa gccgggacta
      901 ctaccacaat gcggtggtgg agaaaatcca ccgggatggt tgtgggctcc gcgattgctg
      961 gcttgcggct gatccctccc aaacgggctt ccatattccc ccgtgtgcgt ttcctttgcc
     1021 gtgctccgcc cttccagcgc gcctgcgcgc tggcgatgaa agaacttgcc cacaaccaag
     1081 caaccgctaa gacgctgaac taaagtaact ttgatatgag ggattggcta cggctgattg
     1141 tgccccccgt tcaggcccac ctaccggagc ttatcctaag agagcattgt gccaagctca
     1201 tggaccatac cgcgccgtgt aaaaagggtg acattccagt tttatcaata atgtgtaatg
     1261 agagtataca gaaaattttc caaaaatgtt gttttataaa aaaattgtaa gcaagcttgt
     1321 cagccaagaa agggtggacg gggcggatac gccccatccg gcgggggatc ggtaaggcgc
     1381 ggacgaagag ctcagccctc tttgacgccc cggagcaggt agccagcgac ggtgcccagg
     1441 atgccggccg cggcgccgcg ctcgataccg ccgaagacgc ccatcaccag gacggccccc
     1501 accgtcaagg tgacgacaaa gcccacgcgg atgaccctgg accgctcctc ttgttcgagg
     1561 atttcaggtt taatggcaat gaggcccaag acgacaagcc ctcccagcac aggggccagt
     1621 gtcgccaaga ccatcggcca aagaatgcgc ggatcttgaa ggttcgatac ccctacgctg
     1681 gtcacagggg gagttgatgt tggttgtacc gtggggcttg gggtaggtga cggggtggag
     1741 gtgggcgtag gcgtggctgt cggcactacc acttcctccg agacgacgct tttcgcaatg
     1801 ggagttccat tgattaaaat cgaggcggcg ttattgagag tatatggacc aggggtcaag
     1861 gttcctttta ttgtgatcac atagtcaacc tggtcatctc tgccgggagg aaggtcttcc
     1921 accacccaga taatcttttc cgcttcaata gttgccccta ctgcatctat cattggtgtt
     1981 ccattcacgt tagcgaccag ttccttacta aaagcatcct caatggtcac acctgtaact
     2041 ggctccgggc ctgtattctg gtacctaatc gtatagctga ccttgtcgcc tgggtcaata
     2101 tttccatccc catcggcgtc atacgccagt tccacattac ggatcttctg gatcgtcaag
     2161 ctcggcgtta cctgcgggac cgccggggag ggaggttcct gtgtgagcgc cggggaggag
     2221 gtgccctcct ggaacgccgg acgggcggct gccggacgtg cgccattgcc agacagcccg
     2281 gcaagcaggg caagggtgag ggccagggca acgaggaata agatctcttt ggtgaatttg
     2341 gatgtcgctt tcatctcaat cgctccattg tgaacaatct aggtatattc ccgagccagt
     2401 cgccgcaccg gttatgaagc cttttatccg ttttccggcc tgcaaaggtc ttatctggct
     2461 agccatccac gtctggtatt catcagatac cagtgtcaag attgagaata acgcaaattt
     2521 tcatgggagt caacacccgg agagcgccaa attaacatga aacttgaata atgtatgtgg
     2581 tgcgcacaac accttgcacg attccagctt atgtacctgc tgcgaagata ttgacaagta
     2641 tgctatacta atcgcagaga acaacgacgc caaactgcgc tctatttaca aactcagaaa
     2701 atcttgcctg cactgctgct ttcactgggt atagcaagtt caaccattgg tggggtgata
     2761 ccatgggtca gcgattggca ctgatcattg gcaacagcgc ttacctggac ggggcgctat
     2821 cacgcttgct gacccccgat gccgacgtgg gggcgctagg ggaaatctta ttagacccgg
     2881 agctgggcgg ctttgacgat gtcaacgtgc tcgtcaacat gtcttcccac atcatccggc
     2941 gctcgatcgc cagctttttc tccaagaagg cccgcgaaga tctgcttttg ttgtatttct
     3001 ctggccacgg cgtgctggat gaccaggggc atctctacct ggcggtgagg gataccgaca
     3061 gcaagctgct gcgcggcacg gcgatttctg cgaactatat caccgacgag atgaacaaca
     3121 gccgctcgca gcgccagttg ctgatcctgg actgctgtca cagcggtgcg tttgcccgcg
     3181 ggacgaaggg ccagcctggc gccagcgtgg gcacggcgtc c
//
LOCUS       DBNGSX010000277         7339 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_993144, whole genome shotgun sequence.
ACCESSION   DBNGSX010000277 DBNGSX010000000
VERSION     DBNGSX010000277.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7339)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7339)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7339
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_993144"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1241)
                     /locus_tag="AC16CB_08380"
     CDS             complement(45..1241)
                     /locus_tag="AC16CB_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR3342525.1"
                     /translation="MADQPSIQSKARTFSLTTVLLLILPLIALGGVIALFLSTGGGLG
                     LSSPAPVEDLSIERTILAPGSIDLVVRNAGPEEVTIAQVVINEAVWPFSVTPNAAISR
                     LSEATVHLDYEWVQGEAYGVTLFTANAIAFDAEIPVAFTTPQPTSRTFWSFTLIGLYV
                     GVIPVYLGLFWFPALRQLGRRWMVFLLAVTAGLLVFLGLDTLAEALELANAVPSPLQG
                     IGLVGIGAVATFFLLDAISRRQVTSGKSEASQRLSLAYMIATGIGLHNLGEGLAIGAA
                     YNVGEIALGAFLVVGFIIQNITEGLGIIAPVLRDRPGASSLALMGLVGGAPAILGAWI
                     GGFAPNASLAVLFLAVGTGAVFEVVYEIAKLIQKDTARQPMPMTVFSGVVAGMLLLWV
                     TGLLIK"
     gene            complement(1339..2364)
                     /locus_tag="AC16CB_08385"
     CDS             complement(1339..2364)
                     /locus_tag="AC16CB_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR3342526.1"
                     /translation="MDEKISRRNFLRVGILGLFGAGGATLLGRGGVQDAGAPPAQTDH
                     VGHDAMPGVVGEVDHARNGFNPTDILTDFDYGEVSTLPSGQTLREYRITSLNKDIEIV
                     PGIEYPAWTYNGRIPGPTIRCTEGDRIRIHFNNGSTHPHTMHFHGIHAAEMDGVPSSG
                     PGVIEPGQTFTYEFDAEPFGLHLYHCHAFPLARHIAKGLYGAFIIDPKEGRPSVDREF
                     VMVMNGFDVDFDDANDFYAVNAIPFHYDQHPIQIRVGEKVRVYLVNILEFDLINSFHL
                     HANFFNYYPTGTSLTPSEFTDTIMQTQAQRGILEFSYKFPGMYMFHAHNTEFAELGWT
                     GHFEVLP"
     gene            2694..4601
                     /gene="acs"
                     /locus_tag="AC16CB_08390"
     CDS             2694..4601
                     /gene="acs"
                     /locus_tag="AC16CB_08390"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetyl-CoA synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HIR3342527.1"
                     /translation="MAQSAKKDTQLEGEVYYPSPEITQAAHIQEYESLYQYSIQDPEG
                     FWGERADELDWYKKWDKVLDDSKAPFFQWFVGGKTNIVHNALDRHLKTYRKNKLALVW
                     EGEPGDMRTFSYFSLHREVCKFANVLRSMGVKKGDIVTIYMPRIPEMVIAMLACAKIG
                     APHSVVYGGFSVEALAERIEDAQSRVLITADGGWLRGKIVALKDIADEAIARQPTIEN
                     CIVVKRTGQDIYMEAGRDYWYHDLMGLPIASITSYTEELDAEDPLFILYTSGTTGRPK
                     GVVHTHGGYMVYTYSTLKYVFDIKDEDRWWCAADPGWVTGHSYIVYGPLINGATSFMY
                     EGAPNHPYPNRWWKMVENHGITILYTAPTAIRGLMRFGEAWPNRHDLSSLRLLGSVGE
                     PINPEAWKWYHTVIGKGRCPIMDTWWQTETGGFMISPLPITPLKPGSATRPLFGIDAE
                     VVDDDGKPVPAGEEGYLVIKKPWPGMLRTILKDPERYKEQYWGKYGNMYQAGDSARKD
                     KDGYIWVIGRIDDVIKVSGYRLGTAEVESALVSHPDVAEAAAIGLPHELKGNAIHTYV
                     ILKAGVEKSDKLAEELRAHVGHEIGPIARPESVTFMDSLPKTRSGKIMRRLLKARALG
                     LPEGDISTLEE"
     gene            4605..5576
                     /locus_tag="AC16CB_08395"
     CDS             4605..5576
                     /locus_tag="AC16CB_08395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3342528.1"
                     /translation="MKNRKFGRTGWMVSDIGYGMWGMGEWTGSDDQESLQSLQRAVDL
                     GCNFFDTAWAYGAGHSEGLLGDLVRANPDRKLYTATKVPPKNRKWPSRREFALDDCYP
                     PDHIRFYVHSSLVNSGLDRFDLVQFHTWEDSWVDDSRWAAAFDDLKREGVVGAVGISI
                     NRWEPWNGVRAVKSGLIDAVQVIYNIFDQNPEDELFPACREMDVGIIARVPFDEGTLT
                     GTLTLDSKWPSGDWRNSYFVPENLKSSVEHAERLRPLVPPGMTMPEMALRFILNNPDV
                     STIIPGMRKLRHVESNIACSDAGPLPEALHQELRDHRWDRTPSWWSQ"
     gene            complement(5685..6428)
                     /locus_tag="AC16CB_08400"
     CDS             complement(5685..6428)
                     /locus_tag="AC16CB_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR3342529.1"
                     /translation="MTTTIVSSIALFIFAAAILYASVGHAGASGYLAVMALFSVAPEI
                     MRPTALALNVLVATIGTIKFYRAGYFSWSVFWPFALTSIPFSFIGGSLTLPSSVYKVI
                     VGLVLLYAAFRLFRTKKDDLNPLGVKKGPLPVALVFGAGIGLLSGLVGVGGGIFLSPL
                     LILMKWADVKQTSGVSAAFILVNSIAGLSGQIASLTHLPGAIPYWAVAAGIGGWIGAE
                     YGSRRLGSLTLRRLLALVLVVAGVKMILI"
     gene            6898..>7339
                     /locus_tag="AC16CB_08405"
     CDS             6898..>7339
                     /locus_tag="AC16CB_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR3342530.1"
                     /translation="MPFKEVPNKVDFPAQERELLDFWKKSDAFQKLRELHANDPHWSF
                     IDGPITANNPMGVHHGWGRTYKDLFQRFWTMRGRKLRYQNGFDCQGLWVEVEVEREMG
                     FRTKKDIEEFGLEQFVRKCKARVLRYAAVQTEQSMRLGYWVDWND"
BASE COUNT         1701 a         2089 c         2090 g         1459 t
ORIGIN      
        1 aggactatgg cagataattg ataaggagtc tctgtgttca tatttcactt gatcaacaac
       61 ccggtcaccc agagcaacaa catccctgct accacacccg agaagacagt catgggcatg
      121 ggctggcgag ctgtatcttt ttgaatcagc ttggcgatct cgtagaccac ctcaaacact
      181 gcgccagtgc ccaccgccag gaaaagcacc gccagggagg cgtttggggc gaagccgcca
      241 atccaggctc ccaggatagc cggcgcgcca ccgaccagcc ccatcaacgc cagcgagcta
      301 gcccctggtc ggtcgcgtag caccggcgcg atgataccca gcccctcggt gatgttctgg
      361 ataataaagc ccaccaccag gaatgcaccc agggcgatct cgccgacgtt atacgccgca
      421 ccgattgcca gcccttcacc cagattgtgc aggccgatgc cagtggcgat catgtaggcc
      481 agagaaaggc gctgggaggc ctcgctcttg ccggaggtca cctgtcggcg ggagatagca
      541 tccagcagga agaaggttgc caccgcccct atgcccacca atccaatccc ttgtagtggg
      601 gagggcacag cattcgccag ttccagggct tctgccaacg tatccaggcc caggaatacc
      661 aacagcccgg cagtgaccgc cagcaggaat accatccatc gccggcccaa ctggcgaagc
      721 gccggaaacc agaacagccc caggtacaca gggatcacgc caacgtacag accaatcagg
      781 gtgaagctcc agaatgtgcg gctggtaggt tgtggcgtag tgaatgccac tggtatctcg
      841 gcgtcgaagg cgatggcgtt ggcagtgaac agggtgacgc cataggcctc gccctgcacc
      901 cactcgtaat ccaggtgaac cgtggcctcc gagaggcgcg agatggcggc gttgggcgtg
      961 acactgaagg gccacaccgc ctcattgatg actacctggg caatggtcac ttcttccgga
     1021 ccggcgttac gcactaccag atcgatactc cccggtgcaa ggattgtcct ttcgatgctc
     1081 aaatcctcca ccggggccgg ggacgaaagc cccagcccgc cgcccgtgct caggaacagg
     1141 gcaatcaccc cacccagggc gatcaatggc aggattagca gcaggacggt ggtcaaacta
     1201 aacgtcctgg ctttcgattg tatacttggt tgatcagcca taaaaaaacc tctaatcgat
     1261 cgtccgcaaa tctgccccaa ataaggctaa caatggttgt tcttcgcgcc cgttagcgta
     1321 gattagcgga taaatcacct atggtaaaac ctcaaaatgc ccggtccagc ccagctcggc
     1381 gaactcggtg ttatgagcgt ggaacatgta catgcccgga aatttgtagc tgaactccag
     1441 gatgccgcgc tgagcctggg tctgcatgat ggtatcggtg aattcgctgg gtgtcaggct
     1501 ggtgccggtc gggtaataat tgaaaaaatt ggcgtgcagg tggaaggagt tgatcaaatc
     1561 gaactccagg atgttgacta agtaaacccg cactttttca cccacacgga tctggatggg
     1621 gtgctggtca taatggaagg ggatggcgtt gacggcatag aaatcgttgg catcgtcaaa
     1681 gtccacatcg aagccattca tcaccatgac gaactcgcga tccaccgacg gtcggccttc
     1741 tttaggatcg atgatgaaag ctccgtacaa ccccttggca atgtgccgcg ccagcggaaa
     1801 ggcatggcag tgatacaggt gtaatccaaa aggctcggcg tcgaactcgt aggtgaaagt
     1861 ctgtcctggc tcgatcacac ctggaccact ggagggcaca ccatccatct ccgcagcatg
     1921 gatgccatgg aagtgcatgg tatgtgggtg ggtgctgccg ttgttaaagt ggatgcggat
     1981 gcggtcgcct tcggtgcagc ggatggtagg gccagggata cgcccattgt atgtccaggc
     2041 gggatattca atacccggca cgatctcgat atccttgttc aaagatgtga tgcgatactc
     2101 gcgcagcgtc tgcccgctgg gcagcgtgga gacctcgcca taatcgaaat cggtcaaaat
     2161 atccgtggga ttgaaaccat tgcgtgcgtg atccacttcg cctaccacgc ccggcatggc
     2221 atcgtgcccg acatgatcgg tttgggcagg aggtgctccg gcatcctgca cgccaccgcg
     2281 ccctaagagg gtggctccgc ccgctccaaa caagcccaat ataccgaccc gcaggaaatt
     2341 tcgtctgctg attttttcgt ccataaccat tcctatcaat aaatacataa gttacgcagt
     2401 tcgagaggtg atggggccct attcaactgc gtaagtccca aaataagaaa tatcattatt
     2461 caacgatatt tttaggtaca actaaatatt attttagttg atagtgttgt tcttgtcaag
     2521 tgtgattgtt tatggctttt cctgtcaaca ttgacaacat gccttccttt tcttaaaatg
     2581 aaggcctata caggttctca ttctctatta ctctattgct ctcaatctac tctgtctagc
     2641 ctgtaatgtg ctatctttaa ccaaatacag caacccacca aaggagagga aacatggctc
     2701 agtccgctaa aaaagatacc cagttagaag gcgaggtgta ttacccctca ccagaaatca
     2761 cccaggcagc tcatattcaa gaatatgagt cgctctacca gtactccatc caagaccctg
     2821 aaggtttctg gggtgagcgc gccgatgagt tagattggta taaaaaatgg gacaaagtgc
     2881 tggacgacag caaggcgcct ttcttccagt ggttcgtggg cggcaagacc aacatcgtgc
     2941 ataacgccct ggaccgccac ttgaagacct accgcaagaa caagctggct ctggtgtggg
     3001 aaggcgagcc gggtgatatg cgcactttct cgtacttttc cttgcaccgc gaagtgtgca
     3061 aatttgccaa tgtgctgcgc agcatgggtg tgaagaaagg cgacatcgtc accatctaca
     3121 tgccgcgcat ccccgagatg gtcatcgcca tgctagcctg cgccaagatt ggcgcccccc
     3181 acagcgtggt gtacggcggc ttcagcgtgg aggcgctagc agagcgaatc gaggacgccc
     3241 agagccgggt gctgatcacc gccgacggcg gctggctgcg cggcaagatc gtcgctttaa
     3301 aggatattgc tgatgaggcc atcgcccgcc agcccaccat cgagaactgc atcgtggtca
     3361 aacgcaccgg gcaggatatc tacatggaag ctgggcgtga ttactggtat cacgacctga
     3421 tgggcctgcc cattgcctcc atcacgtctt ataccgagga gttggatgcc gaagacccgc
     3481 tgtttatcct gtacacttcc ggcactaccg ggcgccccaa gggggtggtg cacacgcacg
     3541 gcggctacat ggtctatacc tactccacgc tgaagtatgt cttcgatatc aaggacgagg
     3601 accgctggtg gtgcgctgca gaccccggct gggtgaccgg gcactcatac atcgtctatg
     3661 gtcccctgat caatggcgcc accagcttca tgtacgaggg tgcccccaac catccttacc
     3721 ccaaccgctg gtggaagatg gtggagaacc acggcatcac catcctgtat accgccccca
     3781 ccgccatccg cggcctgatg cgctttggcg aggcctggcc taatcgtcac gacctgagca
     3841 gcctgcgcct gctgggctcc gtaggcgagc cgatcaaccc tgaggcttgg aaatggtatc
     3901 acaccgtcat cggcaaggga cgctgcccca ttatggatac ctggtggcag accgagacag
     3961 gcggctttat gatctcgcca ctgcccatca ctccgctcaa gcccggctct gccacccgcc
     4021 cgctctttgg aatcgatgcc gaggtggtgg atgacgacgg caagcctgtg cccgcgggag
     4081 aagagggcta cctggtgatc aagaaaccct ggcccggtat gctgcgcacc atcctgaaag
     4141 accccgagcg ctataaggag cagtactggg ggaaatatgg gaacatgtat caggccggcg
     4201 attccgcccg caaagacaag gatggctata tctgggtcat cgggcgtatc gacgatgtca
     4261 tcaaggtcag tggctaccgg ctgggcaccg ccgaggtgga gagcgctctg gtcagccacc
     4321 cagatgtggc ggaggccgct gcgattggtc tacctcacga gctaaagggc aacgccattc
     4381 acacctacgt gatcctcaag gcgggggtag agaagagcga caagctggcg gaggagctgc
     4441 gcgcccacgt cggtcacgag atcggcccca tcgcccgccc cgagagcgtc acttttatgg
     4501 atagcctgcc caaaactcgc tccggtaaga tcatgcgccg ccttctcaaa gcacgtgccc
     4561 tgggcttgcc cgagggagat atcagcacat tggaagagtg atacatgaaa aaccgcaagt
     4621 ttggcagaac gggatggatg gtcagcgaca tcggctatgg catgtggggc atgggcgagt
     4681 ggacaggctc cgacgaccag gaatcgctcc agtccttgca gcgcgccgtc gatttgggct
     4741 gcaacttttt cgacaccgcc tgggcatacg gggctgggca cagcgaaggg ctgttggggg
     4801 acctggtgcg ggcaaacccg gatcgcaagc tgtacaccgc cactaaagtt ccgcccaaga
     4861 accgcaagtg gcccagccgc cgtgaatttg ccttggatga ttgctaccca cccgatcaca
     4921 tccgcttcta cgtgcactca agcctggtaa actccggatt ggatagattc gatctggtgc
     4981 agtttcatac ctgggaagat agctgggtgg acgactcgcg ctgggcggca gccttcgacg
     5041 acctcaagcg cgagggggta gtgggtgcgg taggcatcag catcaaccgc tgggagccgt
     5101 ggaatggtgt cagggcggtg aaatctggct tgatcgatgc agtgcaggtg atctacaaca
     5161 ttttcgacca gaatcccgag gatgagctct tccccgcctg ccgggagatg gacgtaggga
     5221 tcattgcccg cgtccccttc gacgagggca cgctgaccgg caccctgacg ctggacagca
     5281 agtggccctc aggcgactgg cgcaattcct acttcgtgcc ggagaatttg aaaagcagcg
     5341 tggagcatgc cgagcggctc agaccgctcg tgccgcccgg catgaccatg cccgagatgg
     5401 cgctgcgctt catactcaac aaccccgatg tcagcaccat catccctggc atgcgtaaat
     5461 tgcgccatgt ggagagtaac atcgcctgca gcgacgccgg tccattgccc gaggcgctgc
     5521 accaggagct gcgagaccac cgctgggatc gcaccccctc ctggtggtcg caataagaac
     5581 gggtctttat taggacttac gcagttgaag aggaaaggag gggggtttgg gcgggcgatg
     5641 cctgcccaaa cccccctgcc acgtatccaa ctgcgtaacc tcttttatat taagatcatc
     5701 ttcaccccgg caaccaccag gaccagagcc aacaggcggc gcaaggtaag gctgcctaag
     5761 cggcgactgc catattccgc accgatccaa ccgccgatgc ccgccgccac cgcccagtag
     5821 ggtatagcac caggcagatg ggtcaggctg gcgatctgtc ccgacagccc ggcgatcgaa
     5881 ttcaccagga taaacgccgc cgagacaccc gaggtctgct tgacatccgc ccatttcatg
     5941 agaatcagca gtgggctgag gaaaatacct ccgcccacgc ccaccaaacc ggacagcaac
     6001 ccaatcccag ccccgaaaac cagcgccaca gggagcggac ccttctttac tcccaggggg
     6061 ttcagatcat cctttttcgt ccggaagagc ctaaaagcag cgtagagcag caccaggcct
     6121 acaataacct tgtacactga gctgggcagt gtcaggctgc ctccgataaa tgaaaaggga
     6181 atggatgtca gggcaaacgg ccaaaaaacc gaccaggaaa aatatccagc gcggtagaac
     6241 ttgattgtgc caatcgtagc taccaggacg tttagggcca gtgcggtggg tctcatgatc
     6301 tcgggcgcca cgctgaaaag cgccatgaca gctaggtatc cggaggcgcc ggcgtgcccc
     6361 acagaggcgt agaggatggc agccgcgaag atgaatagcg caattgaaga tacgattgtg
     6421 gtggtcaatt tagcccttaa ataaaatggc attatatcac cgccgttgac catctcgcca
     6481 aattatggca taatccaacc caacttaacc gaaaaaggcg atgatggaaa cgagtaagcc
     6541 tgaccaggct ggcagcgaac ccgggagtgg tgtgagccgg ggcagaccat cgggctgaaa
     6601 atccttccgg agccgcaagt cgaacgtccc ccaagtagat gcgccgggcc tgccccccgt
     6661 tatccgggcc agtgattaga gaattagcga tcagacaact gatcactggt atctgttcac
     6721 tgagctgagt tgcccgaagt ctcgggaacc agggtggtac cgcgggagcg atgtttgcga
     6781 ctctcgtccc tgagaggggc gggagttttt ctttttgtga gcgtgaaggg cagggtgatt
     6841 gttatttttt ttcgctgccc atccgctctc gaatttctaa tttcgtttgg agttgctatg
     6901 ccatttaagg aagtgcccaa caaagtagat ttccctgccc aggagcgtga gctgctcgat
     6961 ttctggaaaa agagcgacgc cttccagaag ctgcgcgagc tgcatgcgaa cgaccctcac
     7021 tggtcattta tagacggtcc aattaccgcc aacaacccca tgggcgttca ccatggctgg
     7081 ggacgcacct acaaagatct gttccagcgc ttctggacca tgcgcggccg taagctgcgc
     7141 taccagaatg gcttcgattg ccaggggctg tgggtagagg tcgaagtcga gcgcgagatg
     7201 gggtttcgca ccaagaaaga catcgaggag tttggcttgg agcagttcgt gcgtaagtgc
     7261 aaggcacgcg tgctgcgcta tgcggctgtg cagacagagc agtccatgcg cctcggttac
     7321 tgggtggatt ggaatgacc
//
LOCUS       DBNGSX010000278         8498 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1477214, whole genome shotgun sequence.
ACCESSION   DBNGSX010000278 DBNGSX010000000
VERSION     DBNGSX010000278.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8498)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8498)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8498
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1477214"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="AC16CB_08410"
     CDS             complement(<1..821)
                     /locus_tag="AC16CB_08410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3342531.1"
                     /translation="MTEIPSHAQVVIIGGGVGGCSIAYHLTQMGWKDVVLLERHELTA
                     GSTWHSAGLVGQLRSDANLTRMMSYSTDLYRRLKDETGQDTGWREVGGLRLASSPERM
                     EDLKRLVGMARSFGMPMELLSPQEALDLFPIMNIDGVLGAAFTPNDGMIDPTQLTNAL
                     AVGAKSRGARFYLDTDVEEIRLKNGRVDEVVTNRGSIKTEIVVNATGQWGGEVGKMVG
                     LNLPVVPMAHLYIITKPIEGVRHEFPTLRDPDLLVYWREEVGGLVTGGYERNPEP"
     gene            complement(904..1728)
                     /locus_tag="AC16CB_08415"
     CDS             complement(904..1728)
                     /locus_tag="AC16CB_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3342532.1"
                     /translation="MAKLVRDIMRPGVLTCSPDLTLGQVAVMLHKHHVHALFVTIAEP
                     GEQDSRILGVITDFDLLAGEWLSADRESLAVMRKMTAGELMSSPVDTIEADVPADEAA
                     RHMKEEVIRRLLVTDNGKPVGVISVSDFISSLAAAIPFGRKKVGDVMSDVYLCCRDKT
                     PLVSTARAMADTGWRSVLVVNAQGKPLGLFSGLDLLSLSAEGRADHTTVSEVMHPLLT
                     IAISASLRDAADMMIENHHHRLVVVDPKSPDSMPLGIISSFDIVAEMAHPESVWQK"
     gene            complement(1760..2551)
                     /locus_tag="AC16CB_08420"
     CDS             complement(1760..2551)
                     /locus_tag="AC16CB_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342533.1"
                     /translation="MSGGQIGMGQQDREERERFENELQRILEASNEAGLTLRVIGSLA
                     FQIHCPSYGFLQQAMGRAYTDIDFAAYRKQAKDIKVLMAGLGYSEDREVFIVSEGDRA
                     IFNNPDNGLHVDVFFDKLDFCHLISWNERLEVDSPTIPLAEMILEKMQIVKINEKDVI
                     DTIMLLMEHPLGKVDEETINVDRIALLCAQDWGLWRTTTMNLDKVKQLAHTYSQLTDE
                     QKQQVVSQVEAALERIENEPKNLAWRLRARVGDRVKWYKDVDEVH"
     gene            2831..3625
                     /locus_tag="AC16CB_08425"
     CDS             2831..3625
                     /locus_tag="AC16CB_08425"
                     /inference="COORDINATES: protein motif:HMM:NF012669.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR3342534.1"
                     /translation="MSKPLIPAQRRERIQEYLALHKIVASTDLSEWLGVSEATIRRDL
                     EWMEEEGFIERTHGGAILSQRLPLEPEYRQRAMRCPEEKRLIGLTAAAQIEEGDIVFI
                     NSGSTTTQIIRHIRRGSGITAITNNLYAALEFGETGLELLLLGGEYQHKSNSVAGHFA
                     IDNLSQVYANKAFIGVDGISPRFGYTVPTNVEAELMRMMIDRTKGPIYVVADHTKWGV
                     VSNFEVAPIHKDLKLITDEGLDEHARADLAARTVEVIIASAYPAKI"
     gene            3669..4664
                     /locus_tag="AC16CB_08430"
     CDS             3669..4664
                     /locus_tag="AC16CB_08430"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3342535.1"
                     /translation="MSFFFKKSPKKTTRLFFATDIHGSERTFRKFINAGKFYQANVLV
                     MGGDITGKLLIPIIKEGNGHFRATLQGSIQKIETEAELQQLQERIGILGFYSQIMDED
                     EFRQTQVDPVAVDKLFRQLARQRLDDWVDLAETRLAGTGIKCFVTGGNDDDPDTLEPL
                     YRQGTQSFFGCENKVVYVDDEHSMVSMGYSTPTPWKTPREVSDDELGKLIEEMVAQVP
                     DKGKCIFNFHDPPLDSTLDTCPMLDWSKDPPEPIVKAGQIVLHGAGSQSVRNAIETHQ
                     PMLGLHGHIHESQSVAKIGRTTCVNPGSEYGEGVLRGCLVNFSNGVIEGYQMTSG"
     gene            4832..5146
                     /locus_tag="AC16CB_08435"
     CDS             4832..5146
                     /locus_tag="AC16CB_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342536.1"
                     /translation="MTATTAQAKPEVFTRKASGLVRVMSPYSAFVYNILTMGLIFPWT
                     YLWAPGALPGGKMAWGIILAMLIEIPIAFAYVWLSTALPVLAATTSSKAVSSAAAWLS
                     QW"
     gene            5059..6684
                     /locus_tag="AC16CB_08440"
     CDS             5059..6684
                     /locus_tag="AC16CB_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR3342537.1"
                     /translation="MAVHRPARSGGDYVFESRVFGGGVAFTVVMSGYVIWILQWVALS
                     GWLLAYLGFAPLFLGLGATMGNASLSAIGLWFTTPSGIIITSILNALVSMLILTSGFK
                     NYVRFQWVMWYAVLLAFAVMFAVLFVTPASQFVERLNAFAVASGAGENFYATATAAAQ
                     SAGVDLNPPFSLIATILVAPIAWTSLQWATYSAQQNGEIKDARSFKNQAFIMIGSLIV
                     TGLLLALLAVGLERLAGSEFLYVAGAGYWYALPEATVNGFYLWPNIIAVAVTASPLIV
                     LIIGLGYILNSHQIVHNCYIGMTRVLVAMSLDRLLPDWFSKVDERRHTPVNAHLAYFL
                     GSIPIILAYNLAPGWVGLTLGVTFGCGYVFIITCLAGALLPYRAKEVYEASPGAKYNV
                     SGYVGVLFTLLGVVAFIWTSWILAPDAFGSYPILVWLVRLGSIVAVVLFLYPMRQYIM
                     EWFSGKPMPWLSALGMLGGGFGMAMVVAFLLAPQLGILGNWDFSNFPGGLWSQIIAFG
                     IIVVSAVWYLLVKRAQSARGINVEYAFKEIPPE"
     gene            6864..>8498
                     /locus_tag="AC16CB_08445"
     CDS             6864..>8498
                     /locus_tag="AC16CB_08445"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3342538.1"
                     /translation="MKNQARVVVIGGGIYGVNVAYHLAKLGWTDLVLVEKNEIASGES
                     AQAAGLVTQFATSQTMMQFRKYSIELYSELGLFDHVGSLRLASSKEQLHELQRSVSRA
                     KGIGMDVEIISPAEALKIMPQLSPKDLYGAIYLPRDGHLDPYTTTTNMARLVREMGCE
                     VVTNTRVTGIKLTSKGEVSEVVTTRGSIKCEHVINATGLWGPRLAAMAGLHLPTTPVD
                     HQHIALKAVPGNEFPHSTPCLRDPDNLVYMREEQGGLVIGGYEPNPKERWVDGVPWEH
                     GGSALPPDFDRFEQLFEGAIRRLPFLDQAEIITLVCHPGAYTPDCQPILGPMPGARGF
                     WNTCGMSLNGYGGAGGMGKLMAEWIVYGEPSQDMYAFRATRFGNYFANTTFATERTRE
                     CVKYYYRLKFPHDENEWARPFRVSPLHFRLQEYGAVFGEKYGWERVNFFQPGKLWRMA
                     GADQRQWGWARPPFFERVGQEHEATRERACLYDLTSFGKIDVRGPGALQLLQRLTDND
                     VDKPLGSAIYTQFLNSLGGVEADVTITRLGEDYFRVV"
BASE COUNT         1882 a         2469 c         2243 g         1904 t
ORIGIN      
        1 aacggctctg gattgcgctc gtaaccacca gtgacgagcc cacccacctc ctcgcgccag
       61 tacaccagca aatcggggtc gcgcagcgtg ggaaattcat ggcgcacgcc ctcaatcggc
      121 ttcgtgatga tatacaggtg cgccatgggc accacgggca ggttcaagcc gaccatctta
      181 cccacctcac cgccccactg tcctgtggca ttgactacga tctcggtctt tatgctgccg
      241 cggttggtga cgacctcatc cacacgaccg ttcttcaagc gaatctcttc cacgtcggta
      301 tccagataaa agcgagctcc gcggcttttt gcccctaccg ccagagcatt ggtcagctgg
      361 gtggggtcaa tcatcccgtc gtttggagtg aaagcagcgc ccagcacgcc gtcgatattc
      421 atgattggga agagatcgag cgcctcttgc gggctgagca actccattgg catgccgaac
      481 gaacgcgcca tgcccaccag ccgcttcaga tcttccatgc gttcaggcga ggaggctaag
      541 cgcaggccgc caacttcccg ccagccggta tcctgtcccg tctcgtcttt cagccgccga
      601 tacaggtcgg tgctataaga catcatgcgg gtcaggttgg catcggagcg caactggccc
      661 actaacccag ccgaatgcca ggtggagcca gcggtcaact cgtggcgctc caaaagaact
      721 acgtctttcc aacccatctg cgtgagatga taggcaatgc tgcacccacc tactccacct
      781 ccaataataa cgacctgggc atgcgaaggg atttcagtca tgttgcattc tccttgaaga
      841 caggattgaa taatttaaga tcaagcccta tcccctcctc ttatttttga gacaccctct
      901 aatctacttt tgccacacag actcgggatg agccatctca gctacgatgt caaatgaaga
      961 gataatgccc aaaggcatgc tgtcgggaga tttgggatcg acaacaacca aacggtgatg
     1021 gtggttctcg atcatcatat cggcggcatc acgcaagctg gccgaaatgg cgatggttag
     1081 caggggatgc atcacctcag acacagttgt atgatcggca cgtccttcag cgctcagcga
     1141 gagcaagtcc aacccgctaa acaatcctaa aggctttccc tgagcattca ccaccagcac
     1201 cgagcgccag ccagtatccg ccatagctcg tgcggtggag accaatggcg tcttatcccg
     1261 gcagcacagg taaacgtctg acatcacatc gcctaccttt ttgcgaccaa aaggaattgc
     1321 ggcagccagg ctggagataa aatccgaaac cgatatcacc cccaccggtt tcccattatc
     1381 tgttaccaac aagcgacgaa taacctcttc cttcatgtgg cgggctgctt catccgccgg
     1441 gacatccgct tcgatggtgt ccaccgggct agacattaac tcgccggcgg tcatcttgcg
     1501 catcacagcc agactctcgc gatccgcaga taaccactcc ccggctaata gatcaaaatc
     1561 cgtaatgaca cccaggatac ggctatcctg ttcacccggc tcagcgatag tgacaaacaa
     1621 ggcgtgcacg tgatgtttat gcagcatgac tgccacctgc cccagtgtca gatcaggcga
     1681 gcaagtcaac acgcccggac gcatgatgtc tcgaaccaac tttgccatag cattcctcct
     1741 gaatatttac cggtatggct taatggactt cgtcaacgtc cttgtaccat ttcacgcggt
     1801 caccgacacg ggcgcgcaat cgccaggcca gattcttagg ctcattttcg atgcgttcca
     1861 aggcggcctc tacttgtgag accacttgct gcttttgctc atctgtcaat tgggaatagg
     1921 tatgcgccaa ctgttttact ttatccaagt tcattgtggt ggtgcgccat aatccccagt
     1981 cttgcgcgca aaggagtgca atccggtcca cattgattgt ctcctcgtcc accttgccca
     2041 acgggtgctc catcaacaac ataatggtgt caatgacatc tttctcattg atcttaacga
     2101 tctgcatctt ttccaggatc atctccgcca gaggaatggt gggcgaatcc acctccaggc
     2161 gctcattcca gctgatcaag tggcagaagt ccagcttgtc gaagaacaca tccacatgca
     2221 gaccattatc tggattgttg aaaatagccc gatccccctc gctgacgatg aagacttcgc
     2281 ggtcttcgct gtagcccaac ccagccatta gtaccttgat atcttttgct tgcttgcgat
     2341 aggcagcaaa gtcgatatcg gtgtacgctc gtcccatagc ctgttggaga aatccataac
     2401 tgggacagtg aatttgaaaa gctaaagatc caatgacacg cagggtcaga cctgcttcgt
     2461 tgctggcttc gagaatgcgc tggagctcat tttcaaagcg ctcccgctct tctcgatcct
     2521 gttgacccat ccctatctga ccgcccgaca tgatacactc ctctaaaaca tacggagaaa
     2581 accgctcgat acaaaaggaa cagaccccaa aggtttcatg cgccagcgta gctgctacga
     2641 cgtaatgctg agatgatgcg ccacccgtgg ataaccagcg gggaaacctt tggggtcttc
     2701 tgagacatct cagcgacatt atacacgaaa tgatataaac ttgcaaatct cttcaaaatc
     2761 ttatataatt attcgataat ataatcaaat ttcgtcacaa acgctaagca gaggttgcga
     2821 ttcccacgtc atgagcaaac ccctcatccc tgcccagcgg cgtgaacgta tccaggaata
     2881 tctggcgctg cataaaatcg ttgccagcac cgatctgagt gaatggctgg gtgtttccga
     2941 ggcaacgatt cgccgtgatt tggagtggat ggaggaggag ggtttcatcg aacgcacaca
     3001 cggcggcgcc atcttgagcc agcgcctgcc gcttgagccg gaatacaggc agagggccat
     3061 gcgttgcccg gaagaaaaac gcctgatcgg cttaaccgct gctgctcaaa tagaagaggg
     3121 ggatattgtc ttcatcaata gcggctcaac caccacacaa atcatccgac atatccgtcg
     3181 aggctcaggc attacggcaa tcaccaacaa cctgtacgcc gccttagagt tcggcgaaac
     3241 tggcttagag ctgctcctgc tgggaggcga atatcagcac aaatcgaatt ctgtggctgg
     3301 gcactttgcc atcgacaact tgagccaggt gtacgccaac aaagctttta tcggtgtaga
     3361 tggcatcagt ccaaggttcg gctacaccgt gccaaccaac gtcgaagcag aattgatgcg
     3421 catgatgatc gatcgcacaa aaggccccat ctatgtggtc gcagaccaca ccaaatgggg
     3481 cgtggtctcc aattttgagg ttgccccaat tcataaagac ctcaagctaa tcacagatga
     3541 aggtttagac gaacatgctc gcgccgacct ggctgcccgt acggttgagg ttattattgc
     3601 gagcgcatat ccagcaaaga tctgacaggt tgctgcctgt tttatgatga caatccggag
     3661 gaattaggat gtcttttttc ttcaagaaat cgcctaagaa aaccacccgc ttgtttttcg
     3721 ctaccgatat acatggttcg gagcgtactt tcagaaaatt catcaatgcc ggaaagtttt
     3781 accaagcaaa tgtgctggtc atgggcggag atatcacagg caaactgctt atccccatca
     3841 tcaaagaggg caatggacat tttcgcgcca ccctacaagg cagtatacag aaaatcgaga
     3901 ccgaggcaga gcttcaacag cttcaagaaa gaattggaat actaggcttt tacagccaga
     3961 tcatggacga agacgaattt cgccagacgc aagtcgaccc ggtggcggtg gataaactct
     4021 tccgccagct ggcgcgccag cgcctggatg actgggtaga cctggccgag acacggctgg
     4081 cgggcacagg cattaagtgc ttcgtaaccg gcggcaacga tgacgacccc gataccctgg
     4141 agccgcttta tcgccagggg acgcaatcct tcttcggctg tgagaacaaa gtggtctacg
     4201 tggatgacga gcacagcatg gtcagcatgg gctacagcac cccaaccccg tggaagacgc
     4261 cccgcgaggt aagcgatgat gagctgggca agttgatcga agagatggtg gcgcaggtgc
     4321 cagataaggg caaatgcatc ttcaattttc atgacccgcc gctcgactcc accctggaca
     4381 cctgcccgat gctggattgg agcaaagacc ccccggagcc catcgttaaa gccggtcaga
     4441 ttgttttaca cggtgcaggc agccagtctg tgcgcaacgc catcgaaact caccagccca
     4501 tgctggggtt gcatggtcac atccacgaat cgcaatcggt ggccaagatc gggcgcacaa
     4561 cctgtgtcaa ccctggcagc gaatatggcg aaggggtgct gcgcggctgc ctggtgaatt
     4621 tttccaacgg cgtgatcgaa ggctatcaga tgacgagtgg ctgatactct ggagcctccc
     4681 gcaggtttag aatctgcggg aggttttcac ggaaggaggt aactatcgga gcagataagg
     4741 gttaacttta actagctatt cagcctgata cgttctgcca attggcagtc acaggccagt
     4801 ccacatattc tcaaccaaag gaggatgtac catgaccgca acaaccgccc aagccaagcc
     4861 cgaagtcttt acccgtaaag cctccggcct ggtgcgggtc atgtccccgt attcagcatt
     4921 tgtttacaat atcttgacca tgggattgat ttttccctgg acctatttat gggcacctgg
     4981 ggcgctgccc ggcgggaaga tggcctgggg cattatcctg gctatgctga tcgagatccc
     5041 catcgccttc gcctatgtat ggctgtccac cgccctgccc gttctggcgg cgactacgtc
     5101 ttcgaaagcc gtgtcttcgg cggcggcgtg gctttcacag tggtgatgtc cggctacgtc
     5161 atctggatcc tgcagtgggt ggcgctgtcc ggctggctgc ttgcgtacct gggctttgca
     5221 ccattgttcc ttggcctggg cgctacgatg ggtaacgcct cactatcagc catcggcctc
     5281 tggtttacca ctcccagcgg catcatcatc accagcatat tgaacgcatt ggtctcgatg
     5341 ctcatcctga ccagtggctt caagaactac gtgcgcttcc agtgggtcat gtggtacgcc
     5401 gtgttgctgg cattcgccgt gatgttcgct gtgctgttcg ttaccccggc ctcgcagttc
     5461 gtcgagcgcc tgaacgcttt tgcggttgca tccggtgctg gtgagaattt ctacgcaact
     5521 gccaccgcag cggctcagtc tgctggagtt gatttaaatc cccccttcag cttgatcgcc
     5581 accatacttg tcgctcccat cgcctggacc tccctgcagt gggctaccta ctccgcccag
     5641 cagaacggcg agatcaagga tgcccgctcc ttcaagaacc aggctttcat catgatcggc
     5701 tcgctgatcg tcactggctt gctgctagct ttgctggcgg tgggtctgga gcgactggca
     5761 ggttctgagt tcctttatgt agctggtgca ggctactggt atgcgctgcc tgaagcaacg
     5821 gtgaacggtt tttacctgtg gcccaatatc atcgccgtgg ctgtgactgc cagcccgttg
     5881 atcgtgttga tcatcggcct gggctacatc ctcaactctc accagatcgt ccataactgc
     5941 tacattggca tgacgcgcgt gctggtggcg atgtcgctcg accgcctgct gcccgactgg
     6001 ttcagcaagg tagatgagcg ccgacacaca cccgtgaacg cccacctggc ttacttcttg
     6061 ggcagcatac ctatcatcct ggcgtacaac ttggcaccgg gatgggttgg actgacctta
     6121 ggcgtgacct tcggctgcgg ttacgtcttc atcatcacct gcctggccgg cgcactgctg
     6181 ccctaccgcg ccaaagaagt ctacgaggct tcccccggag caaaatacaa tgtcagcggg
     6241 tatgtaggcg tgctcttcac tctactcggc gtggtggcct tcatctggac ctcctggata
     6301 ctggctcccg acgctttcgg tagctatcct atcctggtct ggctggtgcg cctgggttcc
     6361 atcgtcgccg tcgtcctgtt cctctacccg atgcgccagt acatcatgga atggttcagc
     6421 ggcaagccca tgccctggct gtcagcgctt ggcatgctgg gtggcggctt cggcatggcc
     6481 atggtggtgg ccttcctgct ggctccacaa ctcggtattc tgggcaactg ggacttctcc
     6541 aacttccccg gtggcctctg gtcgcagatc atcgcttttg ggattatcgt tgtctcagca
     6601 gtgtggtacc tgctggtgaa acgagcgcag agcgcgcgcg gcatcaatgt cgaatatgcg
     6661 ttcaaggaga tcccgcccga ataaattaaa ctttgtctcg taactgctta gaccccattt
     6721 ctccgacccc cgcgtgcccg tggaggagcg aagatagccg aaaaaggggt acaccccttt
     6781 ttcggccttt tcctcttgcc tgggggagtg ggataggagg gatgaggaat caataaagtg
     6841 caccttcgag gagatgcaaa accatgaaaa atcaagccag agttgtcgtc attggcgggg
     6901 gcatctacgg cgtcaacgtt gcctaccatc tggcaaaact gggctggact gacctggtcc
     6961 tggttgaaaa aaacgagatt gccagcggag aatctgcaca agccgctggt ctggtaaccc
     7021 agtttgcgac ttcccaaacc atgatgcagt tccgcaagta cagcatcgaa ctttacagcg
     7081 agctgggtct gttcgaccat gtcggcagcc tgcgccttgc ctccagcaaa gaacagttgc
     7141 acgagcttca gcgcagtgtc agccgcgcca agggcatcgg catggatgtg gagatcattt
     7201 cgcccgccga agccctgaaa atcatgcccc aattgagccc caaagatctc tacggggcga
     7261 tttacctgcc acgcgatggt catctcgacc cctacaccac cactacgaat atggctcgcc
     7321 tggtgcgcga aatgggttgc gaggtagtca ccaacacgcg cgtcaccggt attaagctca
     7381 cctccaaggg ggaagtgagt gaggtggtca ccacacgcgg ctcgataaag tgcgagcacg
     7441 tgattaacgc caccggtctt tgggggccgc gcctggctgc tatggctggg ctgcatctgc
     7501 ccaccacccc cgtcgatcac cagcatatcg cccttaaagc ggtgccgggg aacgaattcc
     7561 cgcacagcac cccctgcctg cgcgacccag ataacctggt gtacatgcgc gaggagcagg
     7621 gaggcctggt gatcggcggc tacgaaccca atccaaaaga acgctgggtg gatggcgtgc
     7681 cgtgggagca tggcggctca gccctcccgc ccgattttga ccgcttcgag cagttgtttg
     7741 agggcgcaat ccggcgtctt cccttcctgg atcaggctga gatcatcacc ctggtatgcc
     7801 acccaggtgc ctacacaccc gactgccagc ccatcctcgg gccaatgcct ggagcgcgtg
     7861 gcttttggaa tacctgtggt atgtccctta acggctacgg cggcgctggg ggcatgggca
     7921 agctgatggc ggagtggatc gtatatggag agccgtctca ggacatgtac gctttccgtg
     7981 ctacccgctt cgggaactac tttgctaaca ccacgttcgc cactgaacgc acgcgcgaat
     8041 gtgtcaagta ctactaccgg ctgaaattcc cccatgacga aaatgagtgg gcgcggcctt
     8101 tccgcgtcag cccgctgcat ttccgcttgc aggaatatgg cgctgtattt ggcgagaaat
     8161 acggctggga gcgggtcaac ttcttccagc ctggcaagct atggcgcatg gcaggtgctg
     8221 atcagcgcca gtggggctgg gcgcgcccgc ccttctttga gcgcgtcggt caggaacacg
     8281 aagcgactcg tgagcgcgct tgcctgtacg acctgacttc ctttggaaag atcgacgtgc
     8341 gcggccctgg cgctctccag ctcttacaac gcctgaccga taacgatgtg gataagcccc
     8401 taggcagcgc catctatacc cagttcctca attcgctcgg tggtgtcgag gccgatgtga
     8461 ccatcacccg cctgggcgaa gattacttcc gcgtcgtc
//
LOCUS       DBNGSX010000279         2380 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1893223, whole genome shotgun sequence.
ACCESSION   DBNGSX010000279 DBNGSX010000000
VERSION     DBNGSX010000279.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1893223"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="AC16CB_08450"
     CDS             complement(<1..190)
                     /locus_tag="AC16CB_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR3342539.1"
                     /translation="MPEFERATEYALNRLHSELSPARTYHSYWHTISDVMPAAERLSH
                     IVGISDADERLVRVAAAFH"
     gene            704..1432
                     /locus_tag="AC16CB_08455"
     CDS             704..1432
                     /locus_tag="AC16CB_08455"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR3342540.1"
                     /translation="MSEQENIRLAEKFFQAINAHDLSQNSDHYAPDYLYEAPGVPGQF
                     NQEQSQAYTQGFLDAFPDLHFEIKHKIAQGNYVVINWSATGTHTGPLTTPTGDQLPPT
                     GKKAVVPGSTTYEFKGGKVVRSWVHWDMVTLLAQLGLMPGVEPSQPAQENIGTYRVVD
                     GEKANGHWQVGVNAKGKPVLGLPPVNERMQWLPGQLVQVHLPEADVDGPTKFWVVEGK
                     AYKGNKFNNPQVYVKAGDGELIEG"
     gene            complement(1548..2030)
                     /locus_tag="AC16CB_08460"
     CDS             complement(1548..2030)
                     /locus_tag="AC16CB_08460"
                     /inference="COORDINATES: protein motif:HMM:NF042610.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6789 family protein"
                     /protein_id="HIR3342541.1"
                     /translation="MTNLSSFSQDYPKAGDAAVNGLFGGLVAGVGMAIALVTTGLVTG
                     SSVASTLARFDPSNSSQALTGTLAHLAVSGTYGLIFGLIWSTATRLMRMHPSLRQTIL
                     AGGGYGLMLWLAAHFVLLPGTQSPLRELAIWQFLSAHLVYGLLLGWRVGRSGQQDLSI
                     "
BASE COUNT          607 a          592 c          608 g          573 t
ORIGIN      
        1 catgaaaggc tgccgctacc ctgactagcc gctcgtcggc gtccgagatc ccgacgatat
       61 gtgacagccg ttctgcagct ggcattacat cggagatcgt atgccagtat gaatgatacg
      121 tccgcgcggg ggataattcg ctgtgtaagc ggttgagagc gtattccgta gcacgctcga
      181 attcaggcat ggtactcatc aacgtgtttc tacccagctt tgtgctggtt tcaagtccta
      241 aaaatacatc attacgatta aaccatatcg ataatggcta gcttgtaaag cgatgaccca
      301 gcagagggcg aggctcaatc gtgggctaat ggtcggttaa agtaaaaatc gagtaatttt
      361 aacacatgtg tctggcttgt cgacgcgtat atcccgagat ttatactttt cttatgcttc
      421 catcccgtag gatcttggcc gccaattacc ctgagtgaga tgaagaaacc ctaattattt
      481 gaatattagg taatttagat ctataatata tttattacta cttcggatcg gtaaggaacg
      541 accctgaagg cgcgactttt aggatacgct tttatctccg tctaaccata aaaaggacgc
      601 ggtctaactg gtaactgcct gtgctctcgc catatagtaa tcgtctagcc tgagcatagg
      661 tgatgcttca gcagtattta aattgaaata ggagatatta accatgtcag aacaagagaa
      721 tattcgcctc gccgagaaat tctttcaagc catcaatgcc cacgatcttt cccagaactc
      781 ggatcactat gcgcccgact acctttacga ggcccctggt gtccccggcc aatttaacca
      841 ggagcagtct caagcctata ctcagggatt tttggatgca tttcctgatt tgcactttga
      901 gatcaaacac aagattgccc agggtaatta cgtagtcatc aactggagtg ctaccggaac
      961 ccacactggc ccgctcacca ctccaaccgg tgaccaactt ccccccacgg gtaaaaaggc
     1021 tgttgtccca ggcagcacga cctatgagtt taaaggcggg aaggttgtcc gctcgtgggt
     1081 ccattgggac atggtcaccc tgctggcaca gctcggattg atgccaggtg tcgagccgtc
     1141 acagccagcg caggaaaaca tcgggacgta ccgtgtggtg gatggcgaga aggcaaatgg
     1201 gcactggcaa gttggcgtca acgccaaggg taagccggtc ttagggctcc cgccagtcaa
     1261 cgagcgcatg cagtggttgc ccgggcagct cgtacaggtg cacctgccgg aagctgacgt
     1321 agatggcccc acgaaattct gggtggtaga ggggaaggct tataagggca ataagttcaa
     1381 taacccgcaa gtgtacgtca aagcgggcga tggcgagctc atagagggtt agccctaaga
     1441 aatattggga agtctgctct ggtattcttt ggaagaggtc cctggcgact ttgcggacct
     1501 tccagggacg aataccagcg agcctagtct gcctgctttc aattgcttta tatgctcaaa
     1561 tcctgctggc cgctacgccc aactcgccag cccaataata gcccataaac caggtgagct
     1621 gagaggaact gccagatggc gagttcgcgc aatggcgact gggtgcccgg cagcagcacg
     1681 aaatgcgccg ccagccagag catcaatcca tatcctccac cagcaaggat agtttgcctg
     1741 agggatggat gcattcgcat caggcgagtg gctgtgctcc agatgaggcc aaaaatcagc
     1801 ccgtaagtgc ccgagacagc caggtgggcc agtgttccgg tgagcgcttg actgctgttt
     1861 gatgggtcga agcgcgccag cgtagaggca acagaagaac cggtgacaag accagtcgtc
     1921 accagggcaa ttgccatacc cactccagcg accagcccgc caaacaatcc attgacggcg
     1981 gcatcgccag ctttggggta atcttgagaa aagctgctga gatttgtcat tttcgaatcc
     2041 tctagtctaa gatagcgata tcaaacattg atggatggcc gctaactgcc tttggttatc
     2101 aaggtaacta tattgatatc ttagataaac tttatcaaat agacgttcgg aattcgttac
     2161 gaagagcgtt acgatctcaa ccaaaaattg tggtttttga agcgctgtga tcgtctcaat
     2221 tatccatgtg aactgcacct caccaaactc acgccgggga taagcgggta caattaactc
     2281 ttgttgctca ttgctaatta caggagagtc ttatgccgct gaacatgcag ctttcggtaa
     2341 ttttgatcac gcttgcgctg gttttttaca ccatcggcgt
//
LOCUS       DBNGSX010000280        11291 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_230211, whole genome shotgun sequence.
ACCESSION   DBNGSX010000280 DBNGSX010000000
VERSION     DBNGSX010000280.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11291)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11291)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11291
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_230211"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /gene="ftsZ"
                     /locus_tag="AC16CB_08465"
     CDS             complement(<1..791)
                     /gene="ftsZ"
                     /locus_tag="AC16CB_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR3342542.1"
                     /translation="MKHETNLPNQIESFARIKVVGVGGGGCNAVDRMIDEGLQGVDFV
                     AVNTDAQALMLSKAPRRVRVGEKLTRGLGSGGDPETGRKASEESAEELYEVLRGSDMV
                     FITAGMGGGTGTGGAPIVSQIAKEVGALTIGVVTRPFTFEGARRIQSAESGITKLKEQ
                     ADTLIVIPNDRLLQIVDKRANLQQAFRMADDVLRQGVQGISELITVPGLINLDFADVR
                     TIMSEGGAALMAVGMGKGEDAARIAAEQAISSQLLDITIDGARGIL"
     gene            complement(892..2148)
                     /gene="ftsA"
                     /locus_tag="AC16CB_08470"
     CDS             complement(892..2148)
                     /gene="ftsA"
                     /locus_tag="AC16CB_08470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR3342543.1"
                     /translation="MEQPIVVGIDVGTTKICTLVGRENAPGQLSILGVGIEPSQGIRK
                     GVVVDLAAASQAVARSIEKAERTSGLEITSGLVSMAGSQVTSSNNRGVVGISGRVIDQ
                     EDTERAIDAARAVALPHNREIIHVIQRGFNVDGQDGIRNPIGMHGYRLEVETHIITAS
                     EASVENLRQCVGISGVEVSQFVLNPLASAEVVLNETERQMGCVVVDMGGGTTDMAIFI
                     DGDVWYTAVIGVGGNHITSDIAHGLRLPMSQAEEIKIQHGHAVQAQVGAQESFNVKPF
                     GEQDSLQINRQELAHIIEARVEEIFSLVLQEIKRSGYDGLLPAGMVLTGGCSALPGVR
                     DVASQVLGVSARIAQPENLVGMTDRLNSPAFSTSVGLLRWALMMSEYSPQGVKRQKSA
                     PRVAAGAQGGAFNWENIKNWLRRLLP"
     gene            complement(2184..3182)
                     /locus_tag="AC16CB_08475"
     CDS             complement(2184..3182)
                     /locus_tag="AC16CB_08475"
                     /inference="COORDINATES: protein motif:HMM:NF020067.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR3342544.1"
                     /translation="MSETSRLGSVSHRRQNRRDQGIDQRLREKVREAKARGSSYEKRR
                     SPPVMVRGGIAGPPLPLRKTTNSRAKRRYDLTLSVPGAEMRLPALPQISIDLRLLSGV
                     MVAVLGLLIYYLWNSPSFHVEGAKIIGLQRLNSRDVNAIMNLEGESIFAIDPQVLEQK
                     AREAFPEFALVKVDVGMPANVDITVEERLPILTWRQEGRTVLVDANGIAFPQRDQGDS
                     TPAMVVEAFDSPPIMQNESALAGSPVQFIPVEMVSAILSMSAQAPDETPVVYDSKHGL
                     GWKDTRGWEVYFGDALDIDMKLRVYNAMLKELQDDGVQPVLISVEYIHRPYYRLER"
     gene            complement(3204..4325)
                     /locus_tag="AC16CB_08480"
     CDS             complement(3204..4325)
                     /locus_tag="AC16CB_08480"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR3342545.1"
                     /translation="MSQKDKLRVGVLFGGRSGEHEISLLSARSVLSALDPQKYEVTQI
                     GITHQGVWLVGENVLVAFNQGHKKDLVSAALLPVPTRNDLYAICPTQHGEIFELISKL
                     DVVFPILHGTFGEDGTIQGLFEMADLAYIGAGVVGSSVGMDKGIFKDVMRSNAIPVLE
                     YVIVLRREIENAMEAVIAKAEALASYPLFVKPANLGSSVGVSKCSNRSDLYEGLIDAA
                     RYDRRVLVERGINAREIEVSVLGNDDPQASVVGEVIPSRDFYSYEAKYLDDRSELLIP
                     APISKELSQQVQSLAVQSFKAIDCAGLARVDFLLDKDTNELYLNEINTIPGFTRISMY
                     PKLWDAAGVPYPALVDRLIDLALERKRERDRTVRKYNRV"
     gene            complement(4327..5295)
                     /gene="murB"
                     /locus_tag="AC16CB_08485"
     CDS             complement(4327..5295)
                     /gene="murB"
                     /locus_tag="AC16CB_08485"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR3342546.1"
                     /translation="MFIRFETANVNLESLNRLQASFGQRLQRGVPLDRFTSARVGGTA
                     EILLEVESADELAQAATLLWSEGVAWKILGGGSNVLVSDSGVRGVVVVNRARKVRFDE
                     DSDPPTVWAESGANFGLVARQAASRGLSGLEWAAGIPGTIGGAVVGNAGAHGGDMAGN
                     LIVATILQRNLEDGVPIRVTWPVKRLEYAYRTSQLKRMPGQAVVLEAQLRLQRSTPEG
                     VQAKMDEYVSYRRRTQPPGASMGSMFKNPPGDYAGRLIEAAGLKGVQVGDAQISTLHA
                     NFFINHGEATAADIYALIELAKRAVAEKFGVHLELEIELVGNWVGR"
     gene            complement(5302..5484)
                     /locus_tag="AC16CB_08490"
     CDS             complement(5302..5484)
                     /locus_tag="AC16CB_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342547.1"
                     /translation="MTEKQRKYEAEVQLVKPQILMPESKHEEASMYLVNTRLERVHRT
                     FSRETVVRLINWFKQE"
     gene            complement(5532..6932)
                     /gene="murC"
                     /locus_tag="AC16CB_08495"
     CDS             complement(5532..6932)
                     /gene="murC"
                     /locus_tag="AC16CB_08495"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR3342548.1"
                     /translation="MMNQSMEHVHLIGIGGSGLSAIARVLLERGVVVSGSDRQPSGVT
                     RALQDAGARVFIGHSAGNVQGADLVVRSSAVSDENVEVIAAQGAGIPVLKRAEFLGRI
                     LEGYRVIAVAGTHGKTTTTAMLSWILTTLGESPSFIIGGMARDLGTNARAGSGRLFVI
                     EADEYDHMFLGLSPDIAVVTNVEHDHPDCYPTAEDFYRAFQQFAGRLKPHGILVGCHD
                     DPGARRLLRDAVQTGQRTLSYGLNHWAPLDGPNYAARELVPNARGGFSFDVIQQDRQS
                     AGIQVDLKIPGRHNVSNALACLVVIDCLNLPLEKAAQALGQYSGAGRRFEVRGEARGV
                     IVIDDYAHHPSEIRATLAAARSRFPEKNIWAVWQPHTYSRTRTLLSEFATAFENADHV
                     VVTDIYAARENSPANGFSSQQVVENMVHREANYVPGLSQAADYLLDRVQPEAVVLVLS
                     AGDADQISDLLLRKLG"
     gene            complement(6932..7450)
                     /locus_tag="AC16CB_08500"
     CDS             complement(6932..7450)
                     /locus_tag="AC16CB_08500"
                     /inference="COORDINATES: protein motif:HMM:NF014704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIR3342549.1"
                     /translation="MMTLTFAFWLFAFLFAIIGAMRGWAKELLVTFSVILSLFFLEVL
                     GRYFPPVRDYLAPPNGSAEFWVRAIVVMMLSFFGYQTPNITKLAGTRFARERLADSLL
                     GFFLGLLNGFLIVGTLWYFMEDSNYPFPKYITKPTPPPTLLAYLAPRWLGIPTIYFAI
                     AVSFLFVLVVFI"
     gene            complement(7454..8584)
                     /locus_tag="AC16CB_08505"
     CDS             complement(7454..8584)
                     /locus_tag="AC16CB_08505"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - obsolete lipid glycosylation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HIR3342550.1"
                     /translation="MYPALAVLQAMMNDERQARDDDLRPGSIVHDLLWIGGLGGMEAN
                     LVKQTGVPFKAIPAAGIHGVGWRALPGNAWQLGRGLLAARQLLREYKPDVLFFTGGYV
                     AVPVALAARLPGRGYPRPRSLLYIPDIEPGLALKSLARFADQIAVTTEDSREYFGSHA
                     DVVVTGYPVRNSLRMWDKDQAMEALGLKPDLPVLLVFGGSKGARSINQALWSALTELL
                     NEMQIVHITGERDWHEVEAILERLKASQAEDFYVRYHPYPYLHHEMGAAFTAADLAIC
                     RAGASTLGELPLFGLPAVLVPYPHAWRYQEINAQYLVRRGAAVLVNDASLVEQLLPVV
                     RQLMGNFEKRAQMRLAMSALAQPNAAKQIVRLLDDLSAGLRR"
     gene            complement(8544..9812)
                     /locus_tag="AC16CB_08510"
     CDS             complement(8544..9812)
                     /locus_tag="AC16CB_08510"
                     /inference="COORDINATES: protein motif:HMM:NF013279.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR3342551.1"
                     /translation="MFQTISDVFIKGGKANPAGASSVEARRRTVTLGIDVPLLLVIAT
                     LMIFGSLMVYSASWDYSFLIFGSPTYIFSRQVLWMLLGAGVAALLSFMDYHWWRRLAV
                     PAMGLTIISLVVVLFLSDIRHGAVRTLMDGSIQPSELAKLVTVIYLAVWLYAKQEQLS
                     DVSFGLVPLAGILGVLGGLIFIQPDLSAVITILFLGGTLFFLAGGDLRQIGVMLIIAI
                     LVGWLVVVVNTTGGERVSSYLAGLKDPTNASYHVQRSLEAFVNGGWLGVGIGNARTKL
                     TGLPVPPTDSIFAVVGEETGVFGATVLVILFVIMLWRGLSIARRAPDQLGTLLAAGMS
                     LWLAMEALVNMAVMLNLLPFAGNALPFISAGGSNLVVSFAAIGILMNISRTSVKKKEE
                     GRSFVAVVDLRRRNRRGRVSRTRRTASNDE"
     gene            complement(9782..11164)
                     /gene="murD"
                     /locus_tag="AC16CB_08515"
     CDS             complement(9782..11164)
                     /gene="murD"
                     /locus_tag="AC16CB_08515"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR3342552.1"
                     /translation="MQNDFGGQRVLILGAARQGLALARYLVQHGARVTLNDRTPADKM
                     VNAMASLKDLGESVRWVTGGHPVELLNDADLVCVSGGVPLTNPIVVEAQARGIPLSND
                     SQIFLEVTPCQVIGITGSAGKTTTTSLVGKIAVERVTESVKLQASRRVYVGGNIGLPL
                     IARVDEMAPEDIVIMELSSFQLEIMNRSPQIAAVLNVTPNHLDRHETMAAYVAAKRRI
                     LEYQGVEDVAVLGRDDPGAWGMAGMVMGGLVSFGFGGLESGVGTYIRGNDVLYAGDDS
                     EVWIMDISSVQLRGEHNMQNVLAACAIAAAAGIPPAAMRAGVSGFTGVMHRLEFVRRW
                     GGADWYNDSIATAPERTIAAIRSFEEPIILLAGGRDKDLPWGDFAALVSHRVDHLVLF
                     GEAAEKIGLAMGNYPYTRCANLKEAVQAAVHLVEPGDVVLLSPGGTSFDEFKDFEERG
                     ECFKQLVMSL"
BASE COUNT         2811 a         3370 c         2659 g         2451 t
ORIGIN      
        1 aggatgccgc gggcgccatc gatggtaatg tccaacaatt ggctggagat ggcttgctca
       61 gcggcaatac gggcagcatc ttcacctttg cccataccaa ccgccatcaa agccgcgcca
      121 ccttccgaca tgatggtacg cacatcggca aagtccaagt tgatcaaccc tggtaccgtg
      181 atcagctctg agatcccctg gacaccctgc cgtaacacat catccgccat gcggaaagcc
      241 tgttgcaggt tggcgcgctt gtccacgatt tgcagcaagc ggtcgttcgg gatgacaatc
      301 agggtatccg cctgttcttt cagcttggtg ataccagact cggctgattg gatgcggcgc
      361 gcaccttcaa aagtaaacgg gcgagtaacc acaccaatgg tgagggcacc gacctccttg
      421 gcaatctggg aaacgattgg cgctccgcca gtacctgtgc ctccacccat gcctgcggta
      481 ataaacacca tgtctgagcc gcgcagcacc tcgtacagct cctccgccga ctcttccgag
      541 gcttttcgtc cggtctcggg gtctccgccc gaacccaggc cgcgggtgag tttttcgccc
      601 acacgtacac gcctaggcgc cttggaaagc atcaaagcct gagcatcggt gttcactgcc
      661 acaaaatcta caccctgcaa gccttcatcg atcatgcgat cgacggcatt acacccgccg
      721 ccgccaacgc ctacaacttt aatacgggcg aaggattcta tctgattggg caagttggtc
      781 tcatgtttca ttacaagttc ctcctctata aaattgattt atccgttaaa gatttgcata
      841 gatatgttga tattcgacga ttgatgaatt gaaaatctct tgtcatagga ctcaaggcaa
      901 taaccttctc agccaattct tgatattttc ccaattgaat gcacctccct gcgcccctgc
      961 ggcaacgcgt ggcgcacttt tttgacgttt taccccctga ggggagtact cgctcatcat
     1021 cagcgcccag cgcagcaaac ctacactggt cgagaacgct ggtgagttca accggtcggt
     1081 catgccgacc agattctccg gctgcgcgat gcgggcggac acacccagta cctgcgaggc
     1141 gacatcgcgt acacctggca aggcgctgca gcctccggtg agcaccatcc cggcaggcag
     1201 caggccatca taaccagagc gcttgatctc ctgaagcacc agcgagaaga tctcttccac
     1261 acgagcctcg atgatatggg ctagctcctg gcggttgatc tgcaaactgt cttgctcgcc
     1321 gaatggtttt acattgaatg attcttgcgc tcccacttga gcctggacgg catgcccgtg
     1381 ctggatcttg atctcctctg cctgtgacat gggcaggcgc agaccgtggg cgatatccga
     1441 tgtgatgtgg ttgcctccca caccgatcac ggctgtatac cacacgtcgc catctatgaa
     1501 aatcgccata tccgtggtcc caccgcccat atcgaccacc acacagccca tttgacgctc
     1561 tgtctcgttg agcacgactt cagcagatgc gagtgggttt agtacgaatt gagagacctc
     1621 gacccctgag ataccaacgc actggcgcaa gttctcgacc gatgcctctg aggcggtgat
     1681 gatatgcgtc tccacttcca gacggtatcc atgcatgcca atcggattgc gaatgccatc
     1741 ctggccatcc acattgaagc cacgctggat gacatggatg atctcgcgat tatgcggtaa
     1801 agccacagct cgggcagcat cgatggcgcg ctctgtgtct tcttgatcga tcacccgccc
     1861 gctgatccca acaacaccac gattatttga tgaagtcacc tgggagccag ccatgctaac
     1921 caagccagat gtgatctcca agccagatgt acgctcggcc ttctctatcg aacgagctac
     1981 cgcttgcgaa gcagctgcaa gatcgacgac tacgcccttg cggattccct gagagggctc
     2041 aattcctaca cccaggatac tcaactggcc tggggcattc tctcgcccta ccagcgtaca
     2101 aatcttggtt gtacctacat caattccgac aactatcggt tgctccatca gtagctcgct
     2161 ccattctata acactggcag cgtttatctt tccaggcggt agtaaggcct gtgaatgtac
     2221 tccacgctga tcaagactgg ctgcacgcca tcgtcttgta gttctttgag catcgcatta
     2281 tagacgcgca atttcatatc aatatcaagc gcatctccga agtacacttc ccagccccgg
     2341 gtatctttcc agcccaaacc gtgcttggaa tcgtatacca ctggggtttc atcgggcgct
     2401 tgggcgctca tcgaaaggat cgcagaaacc atctcaacag gaataaactg caccgggctg
     2461 cccgctaggg ctgactcatt ttgcataata ggcggtgaat cgaaagcttc caccaccatc
     2521 gctggggtag aatctccttg atcgcgctgg gggaaagcga ttccattagc atccactagt
     2581 accgtacgcc cttcctgtcg ccaggtcaga attggcaggc gttcttcgac agtgatgtcc
     2641 acattcgcag gcatgcctac atccaccttg accaaggcaa attccgggaa tgcttcccgg
     2701 gctttctgct ccaacacctg ggggtcgatg gcaaagatcg actcaccttc caaattcatg
     2761 atggcgttga cgtcgcggct gttcaacctt tgcaacccga taattttagc accttctacg
     2821 tgaaatgaag gagaattcca tagataatat atgagcaatc ctaataccgc taccataacg
     2881 cccgagagca ggcgcaaatc aatcgatatt tgcggtagag ctgggaggcg catttccgca
     2941 ccaggcacac tcagggttaa atcatagcgg cgctttgccc ggctattcgt ggttttgcgc
     3001 aaaggcaggg gcggcccagc aattccacct cgcaccatca ctggcggaga gcgacgcttc
     3061 tcatacgagg aaccgcgcgc cttggcttca cgaacctttt ctcgaagacg ctggtctata
     3121 ccctgatccc ggcgattttg tcttcggtgg cttactgatc ccaagcgact cgtttctgac
     3181 ataacgatat tctccaatcg ctgttacacc cggttatact tacgcacagt gcggtcccgt
     3241 tctcttttcc gctccaatgc caaatcaatc aatcgatcca ccagtgctgg ataaggaacg
     3301 ccggcagcat cccacaactt ggggtacatg ctgatgcggg tgaatcctgg aatagtgttg
     3361 atctcattca aatacaactc attcgtatct ttatctagta aaaaatccac tcgtgccagc
     3421 ccggcgcaat caatcgcctt gaatgactgt accgctaggg attgaacctg ctgacttagt
     3481 tcttttgaaa taggcgcagg gatcagcaat tcagagcggt catccagata tttggcctca
     3541 taagaataga agtcacgcga ggggataacc tctccgacca cagatgcctg cggatcatca
     3601 ttgcccaaaa cactcacctc gatctcgcga gcgttgatgc ctcgctccac cagaacccgc
     3661 ctgtcgtaac gagcagcatc tatcaagcct tcatatagat cagaacgatt gctgcattta
     3721 cttaccccta ctgatgatcc caggtttgct ggcttgacaa acaacggata agaggccaag
     3781 gcctcggctt tggcaatgac ggcttccatg gcgttctcga tctcgcggcg caacacaatc
     3841 acgtactcta atactgggat agcatttgaa cgcataacat ctttgaaaat acccttgtcc
     3901 attcctaccg atgaacccac cacgccagcc cctatatatg ccagatctgc catttcaaat
     3961 agaccctgga tagtgccatc ctctccaaat gtaccgtgca ggattgggaa aaccacatcc
     4021 aatttggaga ttaattcgaa gatctcgcca tgctgtgtcg ggcagatagc atatagatcg
     4081 ttacgggttg ggacgggcaa gagcgctgca gaaaccaaat cctttttatg gccttggtta
     4141 aacgcaacca gcacattctc tcccaccaac cagacaccct ggtgagttat cccaatctgg
     4201 gttacctcgt atttctgcgg atcgagcgcc gacaacaccg aacgggctga gagtagagag
     4261 atttcatgct ctcccgaccg ccccccaaaa agcaccccga cgcgcaattt atctttttga
     4321 ctcatcttat cgacctaccc aatttcctac caattcgatt tccagttcca ggtgcacacc
     4381 gaatttctca gcaacagctc ttttcgctag ctcaatcagt gcataaatat cagcagcagt
     4441 ggcctctccg tggttaatga aaaaattggc atgcaaagta ctaatttgcg catcacctac
     4501 ttgaacacct ttcaacccag cagcttcaat cagacgaccg gcgtagtctc ctgggggatt
     4561 tttgaacatg gagcccatac ttgcacctgg gggttgtgtg cgacggcgat agctcacgta
     4621 ttcatccatc tttgcctgta ccccttcagg cgtactgcgc tgcaaacgta gttgagcctc
     4681 taaaacaacc gcttgccccg gcatacgctt cagttggctt gtacgataag catactcaag
     4741 ccgttttaca ggccaggtca ctcgaatcgg taccccatcc tctagatttc gttgcaagat
     4801 ggttgccacg atcagattcc cagccatatc tccaccatgg gcaccagcat tacctactac
     4861 cgctccgccg atcgtgccgg gaatgcccgc cgcccattcc aatcccgata atccacggct
     4921 ggcagcctgc cgggcgacca aaccaaagtt tgcacctgat tcagcccaca ctgtcggtgg
     4981 gtcagagtcc tcatcaaaac gcactttccg tgcccggttg acaaccacca cgccacgtac
     5041 acccgagtcg ctcaccagca cattggagcc accgcccagt attttccaag ctacaccctc
     5101 actccataga agcgtagctg cctgcgccag ctcatcagct gattcgacct ccagcaagat
     5161 ctccgccgtg ccgccaaccc gagccgaggt gaagcggtct aatggcacac cgcgttgcag
     5221 gcgctgtcca aaagaggctt gcagacggtt aaggctttcg aggttcacgt tggcagtttc
     5281 aaatcgaatg aacaaagcac gttattcctg cttaaaccaa ttgatcaagc gaacgacagt
     5341 ctcccgcgag aatgtcctat gaacacgttc gagacgagta ttcaccaggt acatgctcgc
     5401 ctcttcgtgt tttgactccg gcatcaatat ttgcggcttg accaactgca cttcagcttc
     5461 atacttgcgt tgtttttcag tcatcctcgt cactcctaca actacggggc tgctataacg
     5521 attcaattag ctcatcccaa cttgcgcagc aataaatcac tgatttggtc agcgtctcca
     5581 gcggagagca ccagcacgac agcctcaggc tggacacgat ccaggaggta atcagccgcc
     5641 tgagatagcc cggggacata attggcttct cggtgcacca tgttttccac aacttgctgc
     5701 gatgaaaagc cattggcagg agagttctcc cgcgccgcat aaatatccgt aaccacaaca
     5761 tggtcagcgt tctcaaacgc cgtggcgaac tcggagagca gggtgcgcgt gcgcgaataa
     5821 gtatgtggtt gccagacagc ccagatgttt ttctctggaa agcgtgaacg agctgcagcc
     5881 agcgttgccc gaatctcact tggatggtgg gcgtaatcat caatgacgat cacaccacgg
     5941 gcttctccgc gcacctcaaa acgtcttcct gcgccactat actgtccgag cgcctgggca
     6001 gccttctcca gagggagatt aagacaatcg atcacaacca agcaagccag cgcgttgctg
     6061 acattgtgcc gtccggggat cttcaaatcc acctggatac ctgctgattg acgatcctgt
     6121 tggatcacat cgaagctgaa gccgccacgc gcatttggca ctagctcccg ggctgcatag
     6181 tttggtccat ccaagggtgc ccagtgattc aagccatatg acaaggtgcg ttgaccagtt
     6241 tgtacagcat cccttaataa gcgccgggcg ccaggatcat catgacagcc caccaaaatg
     6301 ccgtgcggtt tcaaacgacc ggcaaactgc tggaaagccc ggtaaaagtc ctccgctgtc
     6361 ggatagcaat ccggatgatc atgctccacg ttggtcacca cagcgatatc aggcgaaagg
     6421 cctaagaaca tgtggtcgta ctcatcggct tcgatgacaa acaatctacc tgagcctgct
     6481 cgagcattgg tgcccaaatc gcgcgccatg ccaccgataa taaaagatgg agattctccg
     6541 agcgttgtca aaatccagct cagcatggcc gtggtggtag tcttgccgtg cgtgccggca
     6601 actgcaatca cccggtagcc ttccaggatt cgtcccaaga attcggctcg cttaagcacc
     6661 gggatgccgg ctccttgcgc agcaataact tcaacattct catccgagac agccgatgag
     6721 cgcaccacca ggtccgcccc ttgtacatta cccgcactgt ggccgatgaa cacacgtgca
     6781 ccagcgtcct gcagcgcccg ggttacccca gaaggctgcc ggtcggaacc actgaccact
     6841 acgccgcgtt ccaaaaggac gcgggcaatg gccgaaaggc cgcttccgcc gataccaatg
     6901 agatgcacgt gctccatcga ttgattcatc attaaataaa gaccaccaga acaaacagga
     6961 atgagaccgc aattgcgaaa tagatggttg ggatgcccaa ccagcgtggg gcgaggtaag
     7021 ccagcagcgt aggaggcggt gtcggcttgg ttatatactt ggggaacggg tagtttgagt
     7081 cttccataaa ataccacaag gtaccgacaa tcaaaaagcc attcaataac cccaggaaaa
     7141 agcccaggag tgaatctgcc aggcgctcgc gggcgaagcg tgtcccagcc aatttagtta
     7201 tattgggtgt ctggtatcca aagaaggaca gcatcatcac cacgatcgcg cgcacccaaa
     7261 actctgctga accattcggt ggagccagat agtctcgcac tggtggaaaa taacgcccaa
     7321 gtacctccaa gaaaaacagg gacaggataa cgctgaaggt caccaatagt tccttcgccc
     7381 aacctcgcat tgccccaatg atagcgaata aaaaggcaaa caaccagaag gcaaacgtta
     7441 atgtcatcat tgatcacctc cgcagacctg cggacagatc gtctaagagc cttacaatct
     7501 gtttggccgc attgggctgg gctagggcgc tcatcgccaa gcgcatctga gcgcgtttct
     7561 cgaaatttcc catcagctgg cgcaccaccg gcaataactg ttctaccagg gaagcatcat
     7621 tgactaaaac cgccgccccg cgcctgacaa gatattgggc attgatctcc tggtaacgcc
     7681 aggcatgcgg atacgggact aatacggctg gcaggccaaa cagcggcagt tctcctagcg
     7741 tagatgcccc tgcccggcag atcgccagat cagcggcagt aaaggctgcc cccatttcat
     7801 gatgcaagta tgggtagggg tggtagcgga cataaaagtc ttccgcttgc gaggctttca
     7861 accgttcaag gatagcttcc acttcatgcc aatccctctc tccggtgata tgcacgattt
     7921 gcatctcgtt caacagctcc gtgagggcag accacaatgc ttgattaatc gaccgtgcac
     7981 ccttactgcc tccaaagacg agcaagactg gcaaatcggg cttgagaccc aaggcttcca
     8041 tagcctgatc tttatcccac atgcgcaggc tgttacggac gggataccct gtgactacaa
     8101 catctgcatg gctgccgaaa tattcgcggc tatcttccgt ggtgacggct atctggtctg
     8161 caaagcgagc caggctcttc aatgccaagc caggctcaat atcaggtata tatagcaagc
     8221 tacgcggccg ggggtagccg cgtccgggca atcgcgccgc caaagcaacc ggcacagcca
     8281 cgtagccacc ggtaaagaaa agcacatccg gtttatattc gcgaagtaac tgccgcgccg
     8341 caagtaagcc gcgcccaagt tgccaggcat taccaggcag tgcgcgccag cctacgccat
     8401 gaatgcccgc agccgggatc gctttaaagg gcacaccagt ttgcttcacc aggttagcct
     8461 ccatgccacc taaaccacca atccaaagca aatcgtgaac gatggatccg ggacgaaggt
     8521 catcatccct cgcttgtctc tcatcattca tcattgcttg cagtacggcg agtgcgggat
     8581 acacgccccc gccggttccg cctgcgcaaa tcaacaaccg caacgaagga cctcccttct
     8641 tcctttttct tgacagaagt ccgagaaatg ttcatcaaaa ttccaatagc agcaaaagat
     8701 accacaagat tggatccacc agcgctgata aatggcaggg cattcccggc gaagggcagt
     8761 aagttcagca tgactgccat attgactaag gcttccatcg cgagccataa actcatccca
     8821 gctgccagta aggtacccaa ctggtccgga gctctacgag cgatgctcaa cccgcgccac
     8881 agcatgatga cgaacaagat caccaatacc gttgccccaa atacgccggt ctcctcacct
     8941 accacggcga aaatgctgtc agtgggaggc acaggcaggc cggtgagctt ggtgcgcgca
     9001 ttgccgatgc caacgcctaa ccaaccgcca ttgacaaaag cctccaatga acgctggacg
     9061 tgataagacg catttgtggg atcctttaac ccggctagat agctggatac acgctcgcct
     9121 cccgtcgtgt tcaccacgac gaccagccag cctaccagta tggcgatgat aagcataacg
     9181 ccgatctgcc gcaggtcacc accggccaga aagaacagcg taccgcccaa aaataatatg
     9241 gtgatcacag cactcaggtc gggctgtatg aagattagac cacccaggac gcccaggata
     9301 cccgccaggg gaaccagacc gaaacttaca tcgctcaact gctcttgctt agcgtacagc
     9361 caaaccgcca aatagatcac cgtgaccagt ttcgctaact cagagggctg gatggagcca
     9421 tccattagag ttcgcaccgc accatggcga atatcgctga ggaataatac aacgactaac
     9481 gagataatcg tcaagcccat tgcaggcacg gcaaggcgac gccaccagtg ataatccata
     9541 aatgacaaca aagctgccac tccagcacct aacagcatcc aaagcacctg acggctaaaa
     9601 atataagtgg gagaaccaaa aattagaaac gaataatccc aactggctga ataaaccatt
     9661 aaagagccaa agatcatcag tgtggcaatc accagcagca acgggacatc aatgcccaga
     9721 gttacagtgc gtcgacgcgc ctccacagac gaagctcccg cagggttggc tttgccgcct
     9781 tttataaaga catcactaat tgtttgaaac attctccacg ctcctcgaaa tctttaaact
     9841 catcgaagct agtgccgcct ggtgaaagca gcacaacatc ccctggttca accagatgga
     9901 cggctgcttg gaccgcttcc ttcaggttgg cacaccgtgt ataagggtaa ttgcccatcg
     9961 ccagaccaat tttctccgcg gcttccccga aaagcaccag atgatccaca cggtggctga
    10021 ccagtgcagc aaagtcaccc caaggcagat ccttatctcg gccacctgcc aggagtatga
    10081 tcggctcctc gaatgagcgg atagccgcga tggtgcgctc cggtgctgtg gcgatcgagt
    10141 cgttatacca atctgcccca ccccaacggc gcacgaactc caagcggtgc atcacaccag
    10201 taaatccact aacgcctgcg cgcatggctg caggggggat acctgccgcc gcggcgatgg
    10261 cacaggcagc caatacgttc tgcatattgt gctcgcctcg cagttgtacg ctactaatat
    10321 ccatgatcca gacttcagaa tcatctcctg catagagcac atcgtttccg cgaatatagg
    10381 tacccacgcc gctttctagc ccaccaaaac caaaggatac caagcccccc atcaccatgc
    10441 cagccatccc ccaagctccc ggatcatcgc gaccaaggac cgcgacgtct tcaacgcctt
    10501 gatactcgag gatacgtctt tttgcagcca cgtaggctgc cattgtctca tggcggtcga
    10561 ggtggttagg ggttacattt agcacagccg cgatttgcgg agagcgattc ataatctcca
    10621 attgaaaact cgataattcc attatcacta tgtcctcagg tgccatttcg tccacccgag
    10681 cgatgagagg taaaccgata ttaccgccga cgtaaacacg tcttgaggcc tgtaatttaa
    10741 ctgactccgt gactctctcc acggcgatct ttccaaccaa tgatgtcgtt gttgtcttac
    10801 ctgctgaacc ggtgataccg atcacctggc atggagtcac ctcaagaaat atttgcgagt
    10861 cattcgagag cggaatacct ctcgcctgtg cttcaacaac aatcgggttc gtcaggggta
    10921 caccccctga aacacaaacc agatcagcat cattgagtaa ctctaccggg tgcccccccg
    10981 tcacccagcg aacagactcg cccaaatctt taagtgaagc catcgcattg accattttgt
    11041 cagccggtgt gcggtcatta agagtcacgc gcgctccatg ctgcacaagg taacgcgcca
    11101 aagcaagccc ttgccgggct gcacccaata tcagcactct ttgaccgcca aaatcgtttt
    11161 gcatcctgtc taacgttccg aaaaaacata tttaaataac agccaatgcc actccgatca
    11221 gtccggccag caggctgatc aaccagaaac gctgcacgat ctgggtttcg ctccatccag
    11281 agagctcata a
//
LOCUS       DBNGSX010000281        12454 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_230213, whole genome shotgun sequence.
ACCESSION   DBNGSX010000281 DBNGSX010000000
VERSION     DBNGSX010000281.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12454)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12454)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12454
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_230213"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="AC16CB_08520"
     CDS             <1..1092
                     /locus_tag="AC16CB_08520"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HIR3342553.1"
                     /translation="KNTITHETPPPLIRVGGTHRDIGHQIGEACADQVRRSAENARQL
                     LESAYEELQLTWDGAQIQARKYLPFIQERFPQYVEELMGISEGSGVPFDDVVVINALE
                     AVTTDALHLGKCTSLAVNEQRTADGHVLIAHNEDWIPEDEADVFLVHATPNDEPSFLA
                     MTYGGLLPNIGFNEAGIAQCCDSVYPTDSRIGIPRVVVSRAVLATRTLGDAIRTTLSP
                     HRAAGYNHMIVHESGEIYNVEVSAKRFAILYGHEGYAVHTNFYQDFTMQSIEDEPDEL
                     VGTRVRYYRALRLLLGTQQHTLKSLQTIQKDHINFPNSICNHAEHDLDPLDREKTINA
                     LVIDLNVKAMHIAWGNPCENTYHTFHLES"
     gene            complement(1152..2315)
                     /locus_tag="AC16CB_08525"
     CDS             complement(1152..2315)
                     /locus_tag="AC16CB_08525"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR3342554.1"
                     /translation="MTRYFNYADLTWPEVAALPRNTPLIIPLGEHYPLEKLSEYLGNP
                     SSVGLLPPIPYGWEGSGLRLDDQLLEQFLSNLLASLRDDGFSQVYTLTPPGIAQDLGV
                     LRIALSPNKINLPDLPAKTFIIPPDSARSKVVIIPIGHTEQHGLHLPMATDTFIIEAI
                     AQGVTNAAPERAVTLPVMPYGVSTHRPSFAGTLNTGGRAFEDLWVNLVDTLADRGFDR
                     FYLISGHGGNCSFLVNVTKYAGERHRRIFCATAWLYLSGPQGIAAIEKHRQSAIGGMG
                     HAGELETSLILHLRPDLVHMERVVDETDFIATSSYYMDWVEGGALVANPPWDDDTKTG
                     AYGAGSLATAENGDIWLKAAIAEKVNHVDEIHEQQERREARRKKGFGLWADRI"
     gene            complement(2316..2954)
                     /locus_tag="AC16CB_08530"
     CDS             complement(2316..2954)
                     /locus_tag="AC16CB_08530"
                     /inference="COORDINATES: protein motif:HMM:NF015648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR3342555.1"
                     /translation="MAKNYLESLLGEHEKIFLVSRQHWFTLARAIFVEVVLILIIFTA
                     TIAAAISFSPFAVLISAIGFVLLLIPIATMTLDILNWANRQFIITNRRVIQISGIFNK
                     NVIDSNLEKVNDVKMEQSAIGRLFGYGDIEILTASELGVNLFKRIDEPIHFKTAMLNA
                     KERIDREEFADEAEPRAGTIPEMISQLDELRQKGILTDEEFQLKKSELLSKL"
     gene            complement(3437..4678)
                     /locus_tag="AC16CB_08535"
     CDS             complement(3437..4678)
                     /locus_tag="AC16CB_08535"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3342556.1"
                     /translation="MYSNLRYNITVNVLDGAFFGLAWGSASFFTVIPLFVSTMTDSAT
                     LIGLIPAIHAVGWQLPQLLTANRVSQATRIKPFLMWLTLQERLPYLGLAIIALFVPSL
                     GKQSALALTFFVLIWQGLGSGLAANPWQSLIAKIIPSDIRGTFIGGQAAAANILASLG
                     AIVAGFILEKYVQPFDFFYCFVLASVALTISGIFLALTRETEKQVISRASKSTAFLKD
                     LQTILRREPKFRQFLVIRSLLSFATLAFAFYAVYAVRTFGISEARIGVITGVMMATQI
                     AANPIMGWLGDRWGHRVVMEGGVIACIASAFIAWFAPHPNWFFLVFILAGIGNVAVWT
                     IGMTMSLEFGTDEERPSFIGLTNTLVAPSTILAPFLGGWLADTKGYPATFSLNIVCGG
                     IVLILLLLMKERQPSPALSKL"
     gene            complement(4683..5789)
                     /locus_tag="AC16CB_08540"
     CDS             complement(4683..5789)
                     /locus_tag="AC16CB_08540"
                     /inference="COORDINATES: protein motif:HMM:NF024873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HIR3342557.1"
                     /translation="MSTSLGDTYVVESRYPLDYTQGTSLLWTDQPLNLLASWAQDERI
                     NDLAPQAFAFLDTETTGLSGGTGTYAFLIGVGRIQSGEFYLTQFFMRDPVYEPGQLAA
                     LEEFIAPCSALVTFNGKSFDMPLLHTRYLSHGWTPPFSDQAHIDVLHLARRLWRDRLP
                     SRTLPNLELQILGTTRTEEDVPGWMIPQIFFDYLRSGDARPLKSIFYHNAMDVLSLAA
                     LLSHIINVLAEPLNGQVEHSLDIIAMAKLFEEQGFTDLAIQLYLQGIDIGLPEEAAIN
                     TMLRLATIHKRQENFAKAIPLWEQAAKQQQLHAHVELAKYYEHRAQNFQQAIFWTESA
                     LSVVYDPNFPKHEKPFWKSELEHRLKRLHRKHHG"
     gene            complement(6001..8598)
                     /locus_tag="AC16CB_08545"
     CDS             complement(6001..8598)
                     /locus_tag="AC16CB_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3342558.1"
                     /translation="MSIPRLLSRWRTDPSIGSCFEVWHKLDERQGQFDPLPGNLHPHL
                     VSALQSQGISSLYTHQSLSWNLVQQGNHIVVVTGTASGKTLCYNLPVLDKLLREPLSR
                     ALFLFPTRALAQDQRSTLNEFLAHAPDSETQIPLAVYDGDTPSSSRPAIRKKARLVIS
                     NPDMLHAGILPHHTSWAEFFNQLRYVVIDEIHAYRGVFGSHVANVLRRLKRIATHYGS
                     SPQFILTSATIANPKELAELLVESRVDLVDNDGSSRGSRHFLMYNPPVINRELGLRRS
                     ALQESVRLAEDLLAYNVQTIIFGRSRRTVELILTYLRQRTATDHTSESDKDSASRISE
                     PIRGYRSGYLPIQRREIERGLRMGDVRLVVATNALELGIDIGKMGASLLVGYPGTIAA
                     TWQQAGRAGRGEDTALSVLISTADPLDQFLVRHPDYLFERPPENALINPDNPLILLDH
                     LRCAAFELPFRDGENFGSVEASKVGEFLDFLQESGILHYSGGRFFWMSEQYPAQETSL
                     RSASANNVILQTMIDDSPLTIGEVDHGSAHWMVHPGAVYIHEAQTYLVESLDLEQSIA
                     SLQLVETDYYTEPRIETNVILEEKFGESLVQGASKAHGAIQVTAQLTGYRKLRWYTHE
                     LLAFSELALPPTKLLTTGYWIALQESTIERLREVGLWLNDPNQYGPNWRQQRDRARAR
                     DSYRCQVCGTPEGERVHDVHHKIPFRAFTDERGSIDEQAANRLENLITLCPSCHRRAE
                     TAVRVRSGLAGLAFTLRHLAPLFLMCDTRDLGVHADPQSPLGDGQPVVVIYDQIPAGI
                     GFSQRLFELHDELLARARELVLTCECGEGCPSCVGPGGEHGMGGKKETLAILEALVLE
                     DNNLEEIVH"
     gene            complement(8782..9072)
                     /locus_tag="AC16CB_08550"
     CDS             complement(8782..9072)
                     /locus_tag="AC16CB_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR3342559.1"
                     /translation="MKKFVFLTYGYETPTQEIMDAWSNWFASIGDKILDSGNPFGPGK
                     EITHEGIKELPHNKEAITGYMIINAENIDAAVQIAKACPIITSIRVYEAMSM"
     gene            9388..11622
                     /locus_tag="AC16CB_08555"
     CDS             9388..11622
                     /locus_tag="AC16CB_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3342560.1"
                     /translation="MLPQNWVGKTIGGRYQIESIIGQGGMSAVYKANDPNLRRVVAIK
                     LIHSHLSVNPNFVNRFKEEAAAVARLRHPNIVQVHDFNIDDDTYYMVMEYLVGETLQA
                     RLRRLTAAHRFLPYTDLIQFCIQLCDAIGYAHNHELIHRDIKPANIMLDLNGQAILMD
                     FGIVKIVGGDYHTATGATIGTAMYMSPEQIRSERVDERSDIYSLGVTLYEMISGQTPY
                     QADSAITLMMMVLNDPLPDLREIRKEIPENLLAVVEKALAKDPSERFQTMDEMAAALR
                     MAQEQLESTPSVATVVEDDELLQTLPLELPPEEHETVRDTPKEPKVAEASLPERQAVE
                     YSQRDIQRVEEGVSSIPTMREAPIQPDIAPLPSATSKIKGTPKLGSKRIIAAIVALLV
                     LGAASVVSIYFINAQKAPDLQLAPINHPSNPINAQTAQLVVSLGRWETGSYIEDLAYS
                     PVDSLVGTANNRDWVRFSKYGFYGGLWQVDTGSLQNYFLGHTSWIYGVVFSPDGQLFA
                     TASDDSSVIVWQVSDGSQVRKIEGSMGGFTNVDFSPNNLLLAAGSWDGVVGLWQLSDG
                     NLLRTLQGNQSGVRDVEFSPDGKLLASALEDNTIIVWQVSDGSMLHTLQGHTAQIYNL
                     AFSPDGTLLASASEDKTIGIWQISDGSLINKLQGHSEAVSDVAFSPDGSLLASGSLDA
                     TLRLWRLTDGEMFRLLSEDSESIKSVVFSPDGHLLVSAAADGSIRFWGLSEAIPLETE
                     APAN"
     gene            11679..11891
                     /locus_tag="AC16CB_08560"
     CDS             11679..11891
                     /locus_tag="AC16CB_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342561.1"
                     /translation="MPSRMADKGGELRLVYNQNPGKAVWITDDQLVFLGSDGLNIASA
                     PEYEPVIVQPGFRSTEMLLVGRNSFR"
     gene            complement(11911..12132)
                     /locus_tag="AC16CB_08565"
     CDS             complement(11911..12132)
                     /locus_tag="AC16CB_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342562.1"
                     /translation="MDERPGCLSGLIKLALLGWLFDWLQDNFGFGRGVSCTGCGCGFI
                     LLIIFILFACNIIFGTNWLQVWNLMTPLA"
     gene            complement(12268..>12454)
                     /locus_tag="AC16CB_08570"
     CDS             complement(12268..>12454)
                     /locus_tag="AC16CB_08570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012255937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3342563.1"
                     /translation="GMAAAHQSESAVHSHTHRALEEGATQEEIEHAILQGITTIGFPK
                     TMAALTWSKEARKSRQG"
BASE COUNT         3276 a         2859 c         3131 g         3188 t
ORIGIN      
        1 aaaaacacga ttacacatga aacaccaccc ccattgattc gggttggagg tacacacagg
       61 gatattggac accaaatcgg agaggcttgt gctgaccagg tgcgtagatc cgcagagaat
      121 gctcgtcaat tactggaaag cgcctatgaa gagctgcaat taacctggga cggcgcccag
      181 atacaggcaa ggaaatacct gccttttata caggagcgtt ttcctcaata cgttgaagaa
      241 ctaatgggca tttccgaagg ctctggagtg ccatttgatg atgtcgtggt catcaatgca
      301 ttggaagcgg tgacaacgga cgcattgcat ctaggcaaat gcactagctt ggctgttaat
      361 gagcaacgta ctgctgacgg tcatgtgctc attgcccaca atgaagattg gatacccgaa
      421 gacgaggcag atgtatttct cgttcatgcg acacccaacg atgaaccatc ttttctggcg
      481 atgacctatg gggggttgtt accaaatatt ggctttaatg aggcaggcat tgctcaatgt
      541 tgcgattcgg tatatcccac cgatagccgc attggtattc cgcgagtggt ggtctcgcga
      601 gctgtgcttg caacgcgcac attgggagac gcaatccgca ctacgctatc tccacaccgc
      661 gctgcaggat acaaccatat gatcgtccat gagagcgggg aaatatacaa tgttgaagta
      721 tctgcaaaga gatttgctat cttatatggc cacgaaggat acgcggtaca tactaatttt
      781 taccaagatt tcacaatgca atcaatcgaa gacgaacccg atgaacttgt tggcacgcgt
      841 gtacgatatt atcgcgccct acgcctttta ttgggcacac aacagcacac acttaaatcg
      901 cttcagacaa ttcaaaagga ccatatcaac ttcccgaatt caatttgtaa ccacgctgag
      961 cacgatctgg atccgttaga tcgcgagaaa acgatcaacg ctttagtgat cgatctgaat
     1021 gtgaaggcaa tgcatattgc ctggggaaac ccgtgcgaaa atacatatca tacgtttcac
     1081 ctggagtctt agctagatcc aaagattgaa cgtttgaata tttccctact attgccgatg
     1141 tttttttgca atcagattcg gtcagcccag agtccgaatc ccttttttcg ccgggcctcc
     1201 cgacgttcct gctgttcgtg gatttcatcc acgtgattaa ctttttcagc gattgcagcc
     1261 ttgagccaaa tgtccccgtt ttctgcggtt gctaggctgc ctgcgccata agcaccggtt
     1321 ttcgtatcat catcccaggg cgggttagcc accaaagcgc caccttccac ccagtccata
     1381 tagtatgagg aggtggcgat gaagtctgtt tcatcaacaa cccgttccat gtgcaccagg
     1441 tctggtcgaa ggtgcaaaat gagggaggtc tccaactcac ctgcatgtcc cattccccca
     1501 atagcggatt ggcggtgttt ctcgatggcg gcaattccct gaggaccaga taaatacagc
     1561 caggcagtgg cgcaaaagat acgtcggtgc cgttcgccgg catacttggt cacattgacg
     1621 agaaacgaac aattcccgcc atgcccgctg atgagataga aacggtcgaa tccgcgatct
     1681 gccaatgtgt cgacaaggtt tacccacaag tcctcaaaag ctcgcccacc ggtgttgagc
     1741 gtcccggcaa aagaaggacg gtgggtacta accccataag gcatgaccgg aagggtcaca
     1801 gcacgctctg gagcggcgtt ggtcacgccc tgagcgatgg cctcgatgat gaatgtatct
     1861 gttgccatgg gtaagtgtag accatgctgc tccgtatgac caattggaat gatgaccact
     1921 ttgctgcgtg ccgaatccgg cgggatgatg aatgttttag caggtaaatc agggagattt
     1981 attttgtttg gagacaaggc aatccgcagg acgccaagat cctgggcgat gccaggtggg
     2041 gtgagggtat agacttgact gaaaccgtca tcacgcaggc tggctagcag attggaaaga
     2101 aattgttcga gcaattgatc atcaagacgc aacccactac cctcccagcc gtaaggtata
     2161 gggggtaaca gtccaacgct agacggatta cccaagtatt cggagagttt ttccagtggg
     2221 taatgctctc ctaaggggat aatcaggggg gtattgcgtg gcagggcagc cacttccggc
     2281 cacgttaggt ctgcgtaatt aaaatatctc gtcatttata actttgacaa taattcgctt
     2341 ttcttgagtt gaaattcttc atcggtcagg attccctttt gtcgtagttc atccagctga
     2401 gaaatcatct cgggaatcgt gcctgcccga ggttctgcct catccgcgaa ttcttcgcga
     2461 tcgatgcgtt ctttggcatt tagcatggca gtcttgaaat ggattggctc gtctatccgc
     2521 ttgaacagat tgacacctaa ttcactggct gtgaggatct cgatatctcc gtaaccaaat
     2581 aaccgaccaa tcgcggattg ctccattttg acatcgttaa ccttctctaa gtttgagtcg
     2641 atcacgtttt tattgaagat gccggaaatc tgaattactc ggcgattggt aataataaac
     2701 tggcggtttg cccagttgag gatatccaaa gtcatggttg caattggtat caacaagagc
     2761 acgaaaccaa ttgccgagat taggactgca aaaggggaaa aagatattgc agcggcaatt
     2821 gtcgcagtaa agattattag tatgaggacg acttccacaa agatagcccg ggctaaagta
     2881 aaccagtgct gccgggaaac caggaaaatc ttctcatgtt cgccgagaag gctctcgaga
     2941 tagtttttgg ccataatgtt cacctccatg gattatgaaa tatgtgagta tatgcctcga
     3001 ttgctgtttt tccagctcaa atgaaaaaga ataattgaga atactatccc aagtgtgaca
     3061 ccgctccctc agcatgtgca acctgaaggt taattcggca agtgcaggtt gataagttct
     3121 tcatgggatc ctttgagctg ggaggttaga tgttttgact ataacaaaga ctagattgcg
     3181 agtcaagaaa atggcacata aattgtgtag aataagccag gtgcccggat taaccgggca
     3241 cctttgtgtt gcggacagcg atgaatcttg ttgaaataat cgacaataat cacctcctct
     3301 taggatctat tcaacctata acaatgactg ttcaattgaa aaagataaat atatgtatgt
     3361 tgcagtaata attgaattga acagctgaac caagtttagc ataatttaat aaatattcgc
     3421 ctttcacaag tgaaacttag agctttgaaa gcgctggtga gggttggcgc tctttcatta
     3481 acagcaggag aatcaaaacg atgccgccac acacaatgtt gagactgaag gtagcaggat
     3541 atcctttggt atcggctagc caccccccca gaaagggtgc cagaatggtg ctgggagcta
     3601 ccagggtatt tgtaaggcca atgaacgagg ggcgttcttc atcagtgcca aattccaagg
     3661 acatggtcat cccaattgtc cacactgcta cattgcctat tccagcgagt atgaaaacga
     3721 gaaaaaacca gtttgggtga ggggcaaacc aggcgatgaa cgcactagca atgcaggcga
     3781 ttactccacc ttccatgacg actcgatgac cccagcgatc tcccaaccaa cccattatag
     3841 ggttggctgc aatctgtgtt gccatcatga ccccggtgat tacaccgata cgggcttcac
     3901 taatgccaaa tgtgcgcact gcatagacag cataaaaagc aaatgccagg gtggcaaatg
     3961 agagcagtga acggatgacc agaaattgac ggaattttgg ttctctgcgt agtatcgtct
     4021 ggagatcttt gagaaatgcg gtacttttac tggctctaga aattacttgt ttttctgtct
     4081 cacgcgtcag ggccaaaaat atgccggaga tggtcaaggc tacactggct agcacgaagc
     4141 agtagaaaaa atcgaagggt tgaacatact tctcgagtat gaagcctgcc acgatagcac
     4201 ctaaacttgc gagtatattg gcagcagcag cctgtccccc gatgaaggtg ccacggatgt
     4261 cggatgggat gattttagcg attaggcttt gccaaggatt ggcagccaat ccggaaccca
     4321 gaccttgcca aatcagaacg aaaaaggtga gcgctaaggc agattgttta ccaagcgatg
     4381 gcacaaacaa ggctatgata gccaatccca ggtagggtaa cctctcctgg agcgtcaacc
     4441 acatcaaaaa tggcttaatg cgtgtagcct gagatactcg attggcagtt agcaattggg
     4501 gtaattgcca tcccacggcg tgtatagcag ggatcaagcc gatcagtgtc gccgaatctg
     4561 tcattgtgct gacaaatagc gggatgacag taaaaaagga tgcagaaccc caagctagcc
     4621 caaaaaatgc accgtcaagt acattgacgg tgatgttata ccgcaaattg gaatacattt
     4681 gcttatccat gatgtttacg atgcaaacgc ttcaggcgat gctctaattc acttttccag
     4741 aagggttttt catgttttgg aaaattggga tcatacacaa ccgagagtgc agattcggtc
     4801 caaaagatgg cttgctgaaa attttgggcg cggtgctcat aatatttggc taattcgacg
     4861 tgcgcgtgca gttgttgctg tttggcagcc tgctcccata ggggaattgc tttcgcaaag
     4921 ttctcctggc gtttatggat ggttgccagt cgtaacatcg tgtttatagc agcctcttct
     4981 ggcaaaccaa tatctatccc ttggagatat agctggattg cgagatcggt aaaaccctgt
     5041 tcttcaaaca atttagccat ggcgatgatg tctaaactat gttcaacctg accgttgagc
     5101 ggctctgcta gcacgttgat tatgtggctg agcagcgctg ccagcgagag cacatccatg
     5161 gcattatgat aaaaaatgct ctttagagga cgggcatcac cgctacgaag gtaatcgaag
     5221 aaaatttgag ggatcatcca ccctggcaca tcctcctctg tacgcgtagt cccgaggatc
     5281 tgaagttcca ggttagggag tgtccggctg ggtagccggt cacgccacaa gcgacgagcc
     5341 agatggagca catcgatgtg tgcttgatct gaaaagggtg gggtccaacc atgggacaaa
     5401 taacgtgtat ggagcaaagg catatcaaat gacttgccat taaaggtgac caaggccgag
     5461 cagggagcga tgaactcttc gagagcggcg agctggccag gttcgtatac cggatcccgc
     5521 ataaaaaatt gtgttaggta aaattctcct gactgaatac gtcctacacc gatcagaaat
     5581 gcataggtgc ctgttccacc cgataggcca gttgtctccg tatcgaggaa tgcgaaggct
     5641 tgcggtgcga ggtcgttaat gcgctcatct tgcgcccacg aggccagtag atttaggggt
     5701 tgatctgtcc ataaaagcga tgtgccttgc gtatagtcaa gaggatagcg cgactcaacg
     5761 acataggtat ccccaagaga ggtgctcaat atgcgtccat tcagtacacg ttctatcgaa
     5821 tagggattag ttgccggggg aggttttaga tcttttgccc cgacttggac gccaagggat
     5881 tttaattgat cagaaaggga gggcatagag aatgtgaatt ggtggataaa accaaatttg
     5941 tgcgttaggg gaagtttacc ataaatcatc cgattccctt gaggtggaac acttcccaaa
     6001 tcaatgcaca atctcttcta aattattatc ttcaagtacg agggcttcca gaatagccaa
     6061 agtttccttt ttccccccca taccatgttc ccctcccggg cccacacaag atggacagcc
     6121 ttctccacac tcacaggtga gaacaagttc tctggcacgc gcgagcaatt cgtcgtgtaa
     6181 ctcaaaaagg cgttggctaa agccaatgcc agccggaatt tgatcgtaga tcacaaccac
     6241 gggttgtccg tcccccagag gtgattgagg atcagcgtgt acacccagat cgcgtgtgtc
     6301 acacatcagg aacaatggag ccaggtgcct gagcgtaaaa gccaatcccg ccaaaccgct
     6361 ccgcacccgt acagccgtct ctgcccgcct atggcaggac ggacagagag tgatcagatt
     6421 ttccagccgg ttagcagcct gttcgtcaat cgatccacgt tcatctgtga atgctcgaaa
     6481 gggaatttta tggtgcacat catggacgcg ctcgccttct ggagtaccgc aaacctggca
     6541 gcgataggaa tccctcgcac gagctcgatc tcgctgctgg cgccaattcg ggccgtattg
     6601 atttgggtcg tttaaccaaa gtccgacttc ccgcaggcgt tcgatggttg attcttggag
     6661 tgctatccag tatccagtgg ttaataactt tgtgggtggt agggcaagct cactgaatgc
     6721 aagcagctca tgtgtgtacc atctgagctt acgataacct gtcagctgag cggtgacctg
     6781 tatagcacca tgcgctttgg atgcgccttg aaccagagat tctccaaatt tttcctctag
     6841 tattacgttc gtttcaatcc gtggctcagt gtaataatct gtttccacca gttgtaaaga
     6901 ggcaatactt tgttctagat ctagggactc gaccagataa gtctgagctt catggatata
     6961 gacagcccca ggatgaacca tccaatgggc gctgccatgg tcaacctctc caatggtcag
     7021 gggcgaatcg tcgatcatag tttgtaggat gacattattt gctgatgcgc tccgcaaaga
     7081 tgtttcctgg gcaggatatt gttctgacat ccagaagaaa cgcccacctg aatagtgcaa
     7141 gatgcctgat tcttgaagaa aatccaagaa ttcgcccacc ttactagctt caacgctacc
     7201 aaaattttct ccatcccgaa aaggtagctc gaatgctgcg catcgcaagt gatctaatag
     7261 aataagtgga ttatccggat ttatcagggc gttttctggg ggacgttcaa agagataatc
     7321 tgggtggcgt accagaaact ggtctagcgg atcggcggtt gagataagta ccgatagggc
     7381 ggtatcttcg ccgcgaccag cacgtccggc ttgttgccag gtagctgcaa ttgtgcctgg
     7441 gtaaccgact aatagcgatg cgcccatttt cccgatatcg atgcccagtt ccaaagcatt
     7501 ggtggcgaca accaggcgca cgtcacccat gcgcaagccg cgttctattt cgcgacgctg
     7561 gattggcagg taacctgatc gatagccccg tataggttca gatattctcg atgctgaatc
     7621 tttgtccgat tccgaagtgt gatcggttgc tgtgcgttgc ctaagatatg tgaggatcaa
     7681 ctcaactgtt cgccgcgaac gcccaaagat gatagtttgg acgttatagg caagcaagtc
     7741 ctcagccagg cggacactct cctgcaatgc actgcggcgc aatcctagct cgcggttaat
     7801 cacaggaggg ttatacatca aaaaatgcct tgaaccgcgt gaggatccat cattatctac
     7861 caggtcaacg cgagattcaa ccagtagctc tgcaagctct ttagggttag caatcgtcgc
     7921 agaagtgagg atgaattgag gggatgagcc ataatgggta gcgatgcgtt tcaagcggcg
     7981 taacacgttg gcaacatgcg atccgaaaac accacgatat gcatggatct cgtctatgac
     8041 tacatagcgt agttggttga aaaactctgc ccaggaagta tggtgaggta agatgccagc
     8101 gtgcagcata tctgggttgc tgatgaccag cctggctttc ttgcgtatgg cgggacgaga
     8161 cgacgagggt gtatcaccat cataaaccgc gagaggaatt tgggtttcgg aatcaggggc
     8221 atgagcgaga aattcattca aggtcgagcg ttggtcttga gctagagccc gggttgggaa
     8281 taagaagaga gcgcgagata ggggctcgcg cagcaatttg tcgagaaccg gaaggttgta
     8341 gcagagtgtt tttccgctgg cagtgccggt taccacaacg atatgattcc cttgttgaac
     8401 caggttccag gataacgatt ggtgagtgta tagcgagctg attccttgag actgcaatgc
     8461 ggagaccaga tgtggatgaa gattccctgg caacggatca aattggcctt gacgctcatc
     8521 tagcttgtgc cagacctcaa aacaacttcc aatggatggg tcggttctcc acctgctcaa
     8581 taacctggga attgacatat catcatgctt ggaccgcgcc agatatctct tttgtgtcaa
     8641 aatggctgcg catttgcact cgtaggagaa tcttaagaga gatcaaccat tttaaggtga
     8701 tttataactc gttatagagg cgaatgttcc gcctcgctac aattttagtt tagatttatc
     8761 ccgcttaata aacaaatctc attacattga catcgcttcg tatactctta tgctcgtaat
     8821 tatcggacaa gctttggcga tttgtaccgc tgcatcaatg ttttctgcgt taatgatcat
     8881 gtagccagtt atcgcctcct tgttatgtgg caattctttg atcccttcat gcgtgatttc
     8941 ttttccaggt ccaaatgggt tgccgctatc aaggatcttg tctcctattg aagcgaacca
     9001 attgctccag gcatccatta tttcttgggt gggtgtttca tatccgtagg ttaaaaatac
     9061 aaatttcttc attgttcgtc cttacattat tttgtgggat gtgaaaattt accatagttg
     9121 ttagcttcaa gttttagtat aacaagacaa agtgatcttg gaaagttttt gatttcaagc
     9181 tttatatgtg ggtgttattt acacttggtt tggttattta cccagtaggt tgtctaaaaa
     9241 tacggaaaat atatataatt attgcagatc gctcttaagt aaggtggggg gtttttgcag
     9301 cggtttcgcc gctgtaaaaa tttccctaac tagccatatc ggggttgggt tctcgaggta
     9361 gctcgagtaa ctctaaggtg aggatttatg ttgccgcaaa attgggttgg gaagacaatc
     9421 ggcgggcgat atcagatcga atcgattatt gggcaagggg gtatgtctgc tgtgtacaag
     9481 gcgaatgacc ccaacttgcg ccgcgtggta gcaattaagc tgattcactc acatttgtct
     9541 gtaaacccaa actttgtcaa ccgctttaaa gaagaagcag ctgcggtcgc ccgtctacgc
     9601 catcctaata ttgtacaggt ccacgatttc aatatcgatg atgataccta ttatatggtc
     9661 atggagtact tggttggaga aacactgcag gcgagactca ggcggcttac agcggcacat
     9721 cgttttttgc catatacaga tctgatccaa ttctgcatcc agctatgtga tgccatcggc
     9781 tatgctcaca accatgaatt gatccatcga gatattaaac cagctaacat tatgcttgac
     9841 ctgaatggac aagccatctt gatggatttt gggattgtaa aaatcgtggg tggggattat
     9901 cacaccgcta ctggagccac aattggtaca gcgatgtaca tgtctcctga acagatccgc
     9961 agcgagcgcg ttgatgagcg ctcagacata tactcattgg gtgtgacgct ttatgaaatg
    10021 atcagtgggc aaacgcctta ccaggctgat tcagccataa cgctgatgat gatggtgctc
    10081 aatgacccat tacccgattt acgagaaatc cggaaggaaa tccccgagaa cctgttggcg
    10141 gtcgttgaaa aagcattggc caaggatcca agcgagcgtt tccaaacgat ggatgagatg
    10201 gctgctgcgc tgcgaatggc gcaagagcaa ctggaaagca ctccctcggt agccacggtg
    10261 gtcgaggatg acgaacttct acaaacattg ccactcgaat taccgccaga ggaacacgaa
    10321 acggttcgag atacaccaaa agaaccgaag gtggcagaag cttcactacc ggagcgacaa
    10381 gccgttgaat actcccagcg tgatattcaa cgggtggaag agggagtttc cagtatccca
    10441 acgatgagag aagcgccaat ccagccagac attgctcccc tcccatctgc cacatcaaaa
    10501 attaagggaa cacctaagct gggttccaaa cggatcatag ctgcgatagt cgccttgtta
    10561 gtgctcggag cggctagtgt ggtgagcata tattttataa acgcccaaaa agcgccagat
    10621 cttcagcttg cacccattaa ccatccctct aatccaataa atgctcaaac ggctcagttg
    10681 gttgttagcc tggggagatg ggaaacgggt tcatacattg aagatctggc ttactcaccc
    10741 gttgattcat tggttggaac agccaataac cgtgattggg tacgtttctc caaatatggc
    10801 ttctacggcg gtttatggca agtggacacg ggaagcctac aaaattattt tcttgggcac
    10861 acttcatgga tttatggtgt ggttttctct ccagatgggc aactctttgc cacagcgtcc
    10921 gatgattcca gcgtgatcgt ttggcaggta tctgatggaa gccaggtacg caaaattgaa
    10981 ggctcgatgg gtgggtttac aaatgtagat ttttcaccga ataatttatt gctggcagct
    11041 ggttcttggg atggggtagt gggtctatgg caattgagcg atggaaactt gctgcgaacc
    11101 ttacaaggaa accaaagcgg cgttcgagat gtggaattct ctccggatgg caagctgctg
    11161 gcttcagctt tagaggataa caccatcata gtttggcagg ttagcgatgg cagcatgctg
    11221 cacaccttgc agggtcacac tgctcaaata tataatctgg ctttctctcc ggatggcaca
    11281 cttttggctt ctgcctcgga agacaagact atcggaatat ggcagattag tgatggcagt
    11341 ctgataaata agttacaagg ccatagtgaa gccgtatccg atgtggcttt ctcaccggat
    11401 ggttcgctgc tggcctccgg ctctttggat gcaacactaa ggttgtggcg gttgacagac
    11461 ggtgaaatgt ttcgcctctt gtctgaggac tcggagagca ttaagagcgt tgttttttcc
    11521 cctgatggcc atttgctggt atcggctgcg gcggatggaa gtatccggtt ttggggatta
    11581 agtgaagcca tcccgctcga aaccgaagcc cctgccaact aggctgacca cactcacctg
    11641 gcgcaaatct accagccata tcgccagatg gcgatgtttt gccctccagg atggcagata
    11701 agggtggaga gctccgtctg gtctacaatc agaatcctgg caaggctgta tggattacgg
    11761 acgatcagct agttttcctt ggctcagatg gcttgaatat cgcaagcgca ccagaatacg
    11821 agccggtaat cgttcagcct ggctttcgga gcaccgagat gttgctggta gggagaaata
    11881 gtttcaggta attaaattta cctcggcatg ttatgcaagg ggggtcatca agttccaaac
    11941 ctgtagccaa ttcgtgccaa agatgatatt gcatgcaaat aagatgaaga tgattaataa
    12001 gataaaccca catccgcatc cagtacagga tacacctcgc ccaaagccaa aattatcttg
    12061 aagccaatcg aatagccaac ccagcagggc gagtttgatc aacccagata gacaacccgg
    12121 tctttcgtcc atgtttgctc ctcaaagaga ataaaatctt tcttccaatg cgcaagtcct
    12181 ataatttata cgtcctagct cacttggagt cgcggagatt acatatcaaa aagccgatgc
    12241 cttgccagtt attctaaagg attgctgtta tccctggcgt gattttctgg cttcctttga
    12301 ccacgttagg gctgccattg ttttgggaaa tccgatggta gtaatacctt gcaaaatggc
    12361 gtgttcgatc tcttcttgtg tggcaccttc ctcaagggcg cggtgggtgt gtgagtgtac
    12421 cgcgctctca gattggtggg cagctgccat accc
//
LOCUS       DBNGSX010000282         8388 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1893334, whole genome shotgun sequence.
ACCESSION   DBNGSX010000282 DBNGSX010000000
VERSION     DBNGSX010000282.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8388)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8388)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8388
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1893334"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..1429
                     /locus_tag="AC16CB_08575"
     CDS             80..1429
                     /locus_tag="AC16CB_08575"
                     /inference="COORDINATES: protein motif:HMM:NF025711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HIR3342564.1"
                     /translation="MAKTRINCPNCRQPIVADIEQLFDVGVEPSAKQSLLSGLTNVAQ
                     CPHCGYQGSVATPLVYHDPEKELLLTFFPPEMGLTRDEQERLIGGLINKVVNNLPQEK
                     RKGYLLRPQTMLTFQGMLERILEADGITREMIQAQQTKLNLLQRLLNASEESRIEMAR
                     NEDELIDAEFFGMLSRLLETSMASNDRETAQALAELQKSILPETTFGRQLQEQTQEVE
                     AALSSLQEIGQDLTRDKLLDLVIKAPNDTRVSAFVSLARPVMDYTFFQILSDRIDRAR
                     GDGRTRLIQLRERLLDLTRQVDQQLEARAAQSRQLLDNILQAEDVSLAAAQYLPAFDD
                     FFIQELNTALEAARGQGDLEKINDLQRVVQVIQEASSAPPEIAFIEELLDAPDETTRQ
                     KILEDNQDMITPEFLDTLTGLVARVEGGSQDQELVANLKAVHRQSMRYSMQMKFKGQ"
     gene            complement(1430..2098)
                     /locus_tag="AC16CB_08580"
     CDS             complement(1430..2098)
                     /locus_tag="AC16CB_08580"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR3342565.1"
                     /translation="MVIKGEEVPMLMAEAGPLNGQRWPIRGSLIIGRDSDCEIVIPDR
                     QISRRHARITTTPKGVLLEDLGSKNGTYCDGVRVDEPALLQDGTSIQFAFVQTFMFMS
                     SDATLPLENDHLHPVRAETSLLRLELRSRRVWIGNQEILPPLSASQFKLLELLYNNQG
                     KVVTRQELIEAIWGEEQAITVSEQALDALVRRLRERLATFDPSRALIATVRGHGLRLD
                     NPTE"
     gene            2163..3152
                     /locus_tag="AC16CB_08585"
     CDS             2163..3152
                     /locus_tag="AC16CB_08585"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3342566.1"
                     /translation="MPTPLKAGEVLRNRYKIKRIIGQGGMGSIYLADDLRLEGRLCAL
                     KEVEHDRTMPADLYKQTREQFLREATVLARLDHPNLPKVSDFFSMGGRDYLVMDFVPG
                     KDLRTLMVEARQRGAFLSERDVLGWANQLADALVYMHNQNPPILHRDIKPSNLKLTPN
                     GLVKLVDFGLVKILASDEMTITILQGRGTALYTPLEQYGGDTGHTDARSDIYAFGATL
                     YHLLTNKPPVEARELFLNPDRLIPPRQINPDISLRTDRAILWAMNLHPEERPQDVEAF
                     RQALLGEWNPATRPRAPLPGPSLVDLVSSPTELTMVWISAGLVLLSLIFTLIR"
     gene            complement(3141..5837)
                     /locus_tag="AC16CB_08590"
     CDS             complement(3141..5837)
                     /locus_tag="AC16CB_08590"
                     /inference="COORDINATES: protein motif:HMM:NF013126.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HIR3342567.1"
                     /translation="MERAESLLDDLSPEERVGQLFLATFQGSDIDGNSEINDLITDYH
                     IGGVILLARNDNFTADPDELERISSMNRRLQLIRWSAAQQTRITASGESFTPTFVPLF
                     ITIAQEGDGYPYDQILNGLTPLPNEMAMGATWNPDLVSQVGNVLGQELSALGFNLLLG
                     PSLDVLETPNLEGGSSLGTRTFGGDPFWVGKLGQAYISGIHRGSGGQMAVVAKHFPGN
                     GGADRLPEEEVATVRKSLEELQNFDLAPFFEVTGEAQSPDMIADALLTSHIRYQGFQG
                     NIRATTRPISFDQQAFNLLMNLPILAEWRQNGGVMVSDDLGSRAVRRFSELTNPNQPF
                     DARRVALNGFLAGNDLLYLGDIIAGDDPDVNTTTVNILRFFAQKYRDDTAFAQRVDES
                     ALRVLALKYKLYDGFSLNQILPSPDNLELIGKSGEITFEVAQNAATLISPSLEELDGA
                     VPDPPDRTERIVFITNARQTRQCSLCPEQPILAVDAMQQAVLRLYGPEAGRQVTPWFL
                     FSYSYEDLIKMLDGDENFFQFERDIKRANWLVFASLDVNSDDPTTLALHRFLAERPDL
                     FQQKRLIVFAFNAPHYLDATNIAKLTAYYGLYSKTPNFIDAAARLLFRELRPAGALPI
                     SVLGVGYDLISATMPDPNQLIPLMLDLPSESVTNGTSTPEPVPTPEFRVESLIAVRTG
                     VIIDHNGHPVPDGTPVQFIATTNGEVNTLPQIVTTQDGIAFTTIQVNASGSLEIRAES
                     EPAKQSQILHFDIPAINGDITPTATEMPTETPTTMPNPTILPTQMATQEPENLVSPNF
                     RDWIMAILLTSIVGALSYRLAAYIGQVRWGVRSGFLSLIGGLLAYTYLALGAPGSQNL
                     LETAGSWGIVLVTLTGCTAGILAAWSWRAIQSALKSESRSAN"
     gene            complement(5997..6845)
                     /locus_tag="AC16CB_08595"
     CDS             complement(5997..6845)
                     /locus_tag="AC16CB_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342568.1"
                     /translation="MEDITPDEVMVVCIARQIRDGETVAQGLATPLVAAGYLLARATH
                     APNLYFASAIGQSICRYPAPLGLTRIESLWLDRSLMNTGFARVATEILPNLRPKEFFR
                     PGQVDPAGNFNNIAIGKTYLTDVKKRPRLRLPGSGGIPDVTTFLDDIFLYVPRHSRVT
                     FVAHLDVRSGLGHAPERKLGSGARYLISDLGEFDFDGGRMRLTSYHPGTSIERIASRT
                     GFELDIAPDVHETPAPSPEELHILREEIDPLGIRKLELLSGKARHELLHQIINAESAI
                     QVIQAR"
     gene            complement(6833..7792)
                     /locus_tag="AC16CB_08600"
     CDS             complement(6833..7792)
                     /locus_tag="AC16CB_08600"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HIR3342569.1"
                     /translation="MIPYTWIEQARARIAAHILHTPVTFDPELNTYLKWENLQVTGSF
                     KARGAFNKVFTLQAWELERGLVAASAGNHGQGVALAGSKAGAQVLVFASEHAVPAKLD
                     AMRALGAELRLIPGGYSEAERAGLEYSANSGTTWVSPYNDGQVIAGQGTLVLETLEDM
                     PELASATWVVPVSGGGLISGVGAALKEGSAPPQAKLVGVQTEASPFMYGLYHNGTQEN
                     AVELPSLADGLAGPVEEGSITIPLVRQYVDDIVLVNESEITNAIRYAWEKYHERIEGS
                     AAVSLAAILNNRVIDRPTVLVLSGGNIQPKVHQEIVGGEAWKT"
     gene            complement(7789..>8388)
                     /locus_tag="AC16CB_08605"
     CDS             complement(7789..>8388)
                     /locus_tag="AC16CB_08605"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR3342570.1"
                     /translation="RTTGVHGRVIGSKLADLRRLDAGSHFDVAYWGETIPTLDEVFEL
                     VGRNILINVELTNYASPTDHLPEQVAEIVQKHNLAQRVMFSSFNPIALLRIHRKLPQV
                     PIGLLAYDGRKGSWARSSLGELIRYQSLNISLEDAEASLVDRVHRKGRRLLVYTVNQA
                     EDMRRLFQLGVDGIFTDDPLTAKRVLSTGSEQRLKNKES"
BASE COUNT         2053 a         2092 c         2186 g         2057 t
ORIGIN      
        1 ggggcccccc ccccccccaa cccccctgcc acctatccaa ctgcgtaact tctaagttat
       61 atgttttctg gagatataaa tggcaaaaac ccgcattaat tgtccaaact gccgtcaacc
      121 aatcgtcgct gatattgaac agctatttga tgttggtgta gaaccctctg caaagcaaag
      181 cctactttca ggcctaacga atgtagctca atgtccccat tgtggttatc aaggcagcgt
      241 ggcaacccca ctggtctatc atgatccaga aaaggaatta ttgctcacct tctttccccc
      301 tgagatgggc ctaactcgcg atgagcagga gcgattaatt gggggattga tcaataaggt
      361 agtcaacaat ctaccccagg agaagcggaa aggatatttg ttgcgccctc agaccatgct
      421 gaccttccag ggaatgctgg agcgcatcct ggaagctgat gggatcacgc gtgagatgat
      481 ccaggctcaa cagacgaaat tgaacctgtt gcagcgtctt ctgaatgcct ctgaagagtc
      541 tcgcattgag atggcacgta acgaagacga actgatcgac gcagagttct ttggaatgct
      601 tagccggttg cttgagacat ccatggcttc taatgatcgc gagacagccc aagctttagc
      661 tgagcttcaa aagagcatat tgccggagac aacctttggt cgccagttgc aagagcaaac
      721 tcaagaggtg gaggcggctc tatcttcatt gcaagaaatt gggcaggatt taacgcggga
      781 taaattgttg gatctggtga taaaggctcc caatgatacg cgcgtgagtg ctttcgtcag
      841 ccttgccaga ccagttatgg attatacgtt cttccaaatc ctcagcgatc gtatcgacag
      901 agcgcgcgga gatggacgga cccgtttaat tcaattgcgc gagcgtctgc tagatttaac
      961 ccgtcaggtg gaccagcagc ttgaagcccg cgctgctcag tctcgtcagt tgctagataa
     1021 tattctgcaa gctgaagatg ttagcttggc ggcggcgcaa tacctgcctg catttgatga
     1081 cttctttatc caggagttga acacggctct tgaagctgca cgtgggcagg gcgatttaga
     1141 gaaaatcaat gatttgcaaa gggttgtgca ggtcatccaa gaagcatcct ctgcacctcc
     1201 agagatcgct ttcatcgagg aattgcttga cgcgccagac gagactaccc gccagaaaat
     1261 cctagaggat aatcaggata tgattacacc cgaattcttg gatacgctca cgggactcgt
     1321 cgcacgggtg gaaggtggca gtcaggatca ggagctggtg gcaaatctca aggctgtcca
     1381 ccgccaatcc atgcgctact cgatgcagat gaaatttaaa gggcagtgat tattctgtag
     1441 ggttgtcgag tcgaagaccg tgaccgcgaa ccgtggctat caaggcgcga gatggatcaa
     1501 aagtggcaag acgttcacgc aatcgtcgta cgagggcgtc tagagcttgc tcggagacag
     1561 tgatcgcctg ttcttctccc cagatggctt cgatgagctc ctggcgggtc actacttttc
     1621 cttgattatt gtaaagcaac tctaataatt taaattgaga ggctgatagg ggtggcagaa
     1681 tttcctggtt gcctatccac accctccttg agcgcagctc gagtcgcagt aagctagtct
     1741 cggctctcac aggatgaagg tgatcattct caagaggtaa agtggcatct gagctcatga
     1801 acatgaaggt ttgtacaaaa gcgaattgga tactggttcc atcttgcagc aatgctggtt
     1861 catcaactcg cactccatcg caatatgtac cgtttttgct gcccagatct tccaggagta
     1921 ctcctttggg cgttgtggtg atgcgggcgt gacgtcgtga gatttgccga tcaggaatca
     1981 cgatttcaca atctgaatct cgcccgatga ttagactgcc acgaataggc cagcgctgac
     2041 cattcaatgg tccagcttca gccattaaca ttgggacttc ttcacctttt attaccatgt
     2101 ccctgcctcc acgggtataa tatcatactg tgggcatcta tagataatct taatttcaaa
     2161 tcatgccaac gccgctaaaa gcaggagaag tattaagaaa tcgctacaaa ataaaacgga
     2221 tcataggcca agggggtatg ggaagcatct atttagcaga tgatctgcgg ctggaagggc
     2281 ggttgtgtgc gctcaaggag gtcgaacacg accgcaccat gccagccgat ctatacaagc
     2341 aaacccgcga gcagttcctg cgcgaggcta cagtattagc gcgcctggat catcccaacc
     2401 tgcccaaggt ctccgatttt ttctcaatgg gcggtcgtga ttacttggta atggactttg
     2461 ttccgggaaa ggatctgcgc acattgatgg tcgaagcccg ccagcggggg gcatttttat
     2521 ccgagcgtga cgtgctcggt tgggcaaatc aattagctga cgcgttggtt tacatgcata
     2581 accaaaaccc cccaatcctg caccgggata ttaaacctag caatttaaaa ctgacaccca
     2641 atggtctggt taagctggta gattttggat tggtgaaaat cttagcgtcc gacgaaatga
     2701 ccattacgat attgcaggga cgtggcaccg cattgtatac cccattggag caatacggtg
     2761 gggatactgg ccatactgac gcccgcagcg atatatacgc atttggggca acactatacc
     2821 atctgctaac caacaagcca cctgtggaag cgcgcgaatt atttctaaat ccagatcggc
     2881 ttatcccacc tcgccagatt aacccagata tcagcttgcg cacagataga gcgattcttt
     2941 gggcgatgaa tcttcatccg gaggaacgtc ctcaagatgt ggaagccttc cgccaggcac
     3001 ttttaggcga gtggaaccca gccacccggc cgcgtgcccc tcttccagga ccgtctctgg
     3061 tagatttggt atcctctccc accgaattga caatggtgtg gatttcggca ggcctggttt
     3121 tgttgagtct gatttttacg ctaattcgct gatctgctct cggattttaa tgctgattgg
     3181 attgcccgcc aactccaggc agctaatatt cccgcagtgc atccggtaag agtaaccaag
     3241 acaattcccc agcttccagc tgtttccaat agattttggc tgccaggcgc gcctagggcg
     3301 agataggtgt aggctagcag gccaccgatt aaagataaaa atccgctacg aacgccccat
     3361 ctgacctgac caatataggc ggccaatcga tacgataatg cacccacaat agacgtcaaa
     3421 aggatggcca tgatccaatc tcggaaattg gggctgacca aattttcggg ttcttgggtt
     3481 gccatttggg ttggtagtat tgttgggttg ggcatggttg tgggggtttc agtaggcatc
     3541 tcagttgcag tgggagttat atctccattt attgctggta tatcgaagtg taatatttgc
     3601 gattgtttgg ctggttcact ttcagcgcga atttccagcg accccgaggc attcacctga
     3661 atggtggtaa aggcgatacc gtcctgggtt gttacgattt ggggtaatgt attgacttca
     3721 ccgttggtgg ttgcgatgaa ctgtacaggt gtcccatccg gcactgggtg accgttgtga
     3781 tcgatgatca ctccagtgcg cactgctatc aggctttcta cccgaaactc cggagttggg
     3841 acaggttctg gcgtactggt tccattggtg acagattccg aagggagatc gagcatgagt
     3901 gggattagct ggttagggtc cggcatggtc gcagatatca gatcataacc cacacccaga
     3961 actgaaattg gcaaggctcc cgccggacga agctcgcgga agagcaggcg ggccgctgcg
     4021 tcgatgaaat ttggggtttt gctgtacagc ccgtaatagg cagtgagctt ggcgatattg
     4081 gttgcatcta aataatgcgg ggcattgaat gcaaagacga tcagcctctt ttgctgaaag
     4141 aggtccggtc gttctgccag gaagcggtgg agagccagcg ttgtagggtc atctgaattc
     4201 acatcgagag aggcaaagac cagccaattg gctcgcttga tatctctttc aaactggaaa
     4261 aaattctcat cgccatccag catcttgatc aggtcttcat aagaatagga aaacaaaaac
     4321 cagggggtca cctgccgacc agcttctggt ccataaagcc tgaggacagc ttgttgcatg
     4381 gcatctacgg ctaaaatcgg ctgctcagga cataggctgc attgacgagt ttgacgggca
     4441 ttggtaatga agacgatgcg ctcggtgcgg tctggggggt caggtacggc cccatccaat
     4501 tcttctagtg aggggctgat aagcgtagcg gcattttgag ccacctcgaa ggtaatttcg
     4561 ccggatttac cgatcaattc caggttatct ggcgaaggga gaatctggtt gagcgaaaaa
     4621 ccatcataca gcttatattt gagtgccagg acccgcagtg ctgattcgtc aacccgttgg
     4681 gcaaaagctg tatcatcgcg gtatttctgg gcaaagaagc gcaggatatt gacggtagtc
     4741 gtattgacgt cgggatcgtc accagcgatg atgtcgccga ggtagagcaa atcgtttcca
     4801 gcaagaaagc cattcagagc cacgcggcgc gcgtcaaatg gttggttagg gtttgtcaat
     4861 tccgagaagc ggcgtactgc ccggcttcca agatcatcag ataccatgac gcccccgttt
     4921 tgccgccact cagccagtat gggtagattc attaataaat taaatgcttg ctggtcaaag
     4981 ctgatcgggc gcgtggtagc gcgtatattt ccctggaatc cctgataacg gatatgtgaa
     5041 gtgagtaaag catcggcaat catatcggga gattgagcct caccagtgac ctcgaagaac
     5101 ggtgctaggt caaagttttg cagctcttct agggacttgc gtactgtagc tacctcttct
     5161 tcaggcaagc gatcggcgcc accatttcct ggaaagtgct tggcgaccac cgccatctgc
     5221 cctccgctcc ctcggtggat gccgctgatg taagcctgtc ccaatttacc cacccaaaac
     5281 gggtctcccc caaacgtgcg cgttcctaag ctgctacccc cttcgagatt cggtgtttct
     5341 agaacatcca gcgatggtcc taatagcaaa ttaaacccta acgcagacag ctcttgtcct
     5401 aatacatttc ctacctgaga gactaaatca ggattccagg tggctcccat ggccatttcg
     5461 ttgggcaggg gagtgagacc attcaaaatc tggtcgtagg gatagccatc gccttcttgg
     5521 gcgattgtta taaataaggg gacgaatgtg ggcgtgaaac tctccccaga cgcagtaata
     5581 cgcgtttgtt gagctgcaga ccagcggatc agctgtaatc ggcgattcat ggatgagatg
     5641 cgttccagct catctggatc tgctgtaaag ttgtcgttgc gcgcaaggag gatgacgccg
     5701 ccaatatgat agtcggtgat cagatcatta atttccgagt taccatcgat gtctgatccc
     5761 tgaaaagtcg ccaggaagag ctgaccgacg cgttcctctg gagagagatc atctagcaag
     5821 gattcagctc gctccattgg ggttgccacc tgataagctg gcgaagcttg gacactagcc
     5881 aaaggattaa tgaatgccag gagtatcgat attaatacaa taaatgtaat ccacccggaa
     5941 gtcgatgaac tcccagtaga ttgactccga gtaagatgtg aaagaaacct catgatttat
     6001 cgagcctgaa tgacttggat agctgattct gcattgataa tctggtgcaa taattcatgc
     6061 ctagctttac cgcttaataa ctccagcttg cggatgccca acgggtcgat ctcctctcgt
     6121 aggatatgga gttcttcagg tgatggagct ggtgtctcgt gcacatcagg ggcaatatca
     6181 agttcgaagc cagtacggga cgctatgcgc tcaatgctgg ttccagggtg gtagctggtc
     6241 aagcgcatac gacccccatc gaagtcgaac tcgcccaaat cggagatcag gtatcgggcc
     6301 cctgagccga gttttctttc gggagcgtga cccagtccag aacgcacatc caggtgtgca
     6361 acaaacgtca cgcgcgagtg gcgtggcacg taaagaaata tatcgtcaag aaaagtggtc
     6421 acatctggga tgcctccaga gccgggtaat cgcaggcggg gccgcttttt tacatccgtg
     6481 aggtaagtct tgcctatggc tatgttatta aaattacctg ccggatcaac ctgtccaggg
     6541 cggaagaact cctttggcct cagattggga agtatttcag tagctacgcg ggcgaatcca
     6601 gtattcatta aagagcgatc taaccaaagg ctttcgattc gggtcagccc caatggcgcg
     6661 ggatagcggc aaatgctttg tccgattgca gaggcaaagt acaaattggg agcgtgggta
     6721 gctcgtgcca gcaagtaacc ggctgcaacg agtggagttg ccaaaccctg ggcaacagtt
     6781 tcgccatctc gaatttggcg agcaatacat accaccatga cctcatctgg ggttatgtct
     6841 tccacgcctc gcctcccaca atttcttggt gcactttagg ctggatgttt ccgccagata
     6901 gaactaaaac cgtagggcga tctatcactc tattattgag gatagctgcc agacttacgg
     6961 ctgccgatcc ttctatacgt tcatggtact tttcccaggc gtaacggata gcgttggtga
     7021 tttccgattc atttaccaag actatatcgt ccacatattg tcgtactaat ggaatggtaa
     7081 tagagccttc ctcaacggga cccgccaatc catcggctaa gctgggcagt tcaacagcat
     7141 tctcttgagt gccattgtga taaagaccat acataaatgg cgaggcttcg gtttgaactc
     7201 caaccagctt agcttgagga ggtgcacttc cctctttgag cgcagcaccc accccagaga
     7261 ttaatccacc accgctgaca ggcaccaccc aagtggcgct cgccagttct ggcatatctt
     7321 ccagcgtttc aagcaccagc gtaccttgcc cggcgattac ctgcccgtca ttatagggcg
     7381 aaacccaggt tgtcccgcta ttcgcagaat attccaaccc tgctctttca gcctcactgt
     7441 atccgccggg aattagccgc aattccgcac ctaaggctcg catagcgtcc agcttggcag
     7501 gcacggcatg ttctgaggca aataccagaa cttgggcacc tgccttggaa ccagccaaag
     7561 cgaccccttg tccatgattc ccagccgaag ccgctaccag tccccgttct aattcccaag
     7621 cttgcagggt gaacactttg ttgaatgccc cacgcgcctt gaatgaacct gtgacctgca
     7681 gattttccca cttcaggtag gtgtttaatt ctggatcaaa tgtgactggt gtgtgtaaaa
     7741 tgtgcgcagc tatacgcgcc cgcgcttgct caatccaggt gtatgggatc atgattcctt
     7801 gtttttcagg cgttgctcgc tgcctgtgga taaaaccctc ttcgcagtta gaggatcgtc
     7861 ggtgaaaata ccgtccaccc caagctggaa gaggcggcgc atatcttcag cttgattgac
     7921 agtgtatacc aataggcggc gacctttccg gtgaactcgg tctaccaggc tagcttcagc
     7981 gtcttcaagg gagatattca gcgactggta gcgaattagc tctcctagcg aggagcgtgc
     8041 ccaggagccc ttgcgcccat cataagctaa taacccgatg ggtacctgcg gaagtttgcg
     8101 atggatgcgt agcagggcaa tggggttgaa agatgagaac atcacccgct gtgcgaggtt
     8161 gtgtttctgc acgatctcgg cgacttgctc aggcaggtga tcggtgggag aggcgtagtt
     8221 ggtcagttca acgttgatta agatattcct gcccaccagc tcgaaaacct cgtccaaggt
     8281 cgggatcgtc tcaccccaat aagccacatc gaagtggctg cctgcatcta ggcggcgtaa
     8341 atcagctaat ttggagccaa taacccgtcc atgaacgccg gtcgttcg
//
LOCUS       DBNGSX010000283         9645 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_924282, whole genome shotgun sequence.
ACCESSION   DBNGSX010000283 DBNGSX010000000
VERSION     DBNGSX010000283.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9645)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9645)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9645
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_924282"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="AC16CB_08610"
     CDS             <1..381
                     /locus_tag="AC16CB_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342571.1"
                     /translation="ADFGLVGVEVRSIPQDDLNILREELQSYCDSQGLELQIPVMENG
                     VVCDPQNQYLKSLLQAVENLSGEKPRIGKKLPGTSARFAPGGQGVVWGQSGLGPHAAN
                     ERHFIPSIEPYYRALGEFGRLLKG"
     gene            378..992
                     /locus_tag="AC16CB_08615"
     CDS             378..992
                     /locus_tag="AC16CB_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013056255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HIR3342572.1"
                     /translation="MSIAFLLKGLVIGFSIAAPVGPIGVLCIKRTLTNGRMSGLVSGL
                     GAATADALYGSIAGFGLTLISAFLLDQQTWIRLVGGAFLCYLGLKTFISKPAEQDVRA
                     HGGNLISAYLSTFVLTLTNPLTILSFAAIFAGLGLGATDGDFASAGILVSGVFLGSAA
                     WWLLLSGGVSLLRQRLDQQVMAWVNRASGVIILGFGVAALWKLI"
     gene            1020..2474
                     /locus_tag="AC16CB_08620"
     CDS             1020..2474
                     /locus_tag="AC16CB_08620"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR3342573.1"
                     /translation="MQERRDSRTLVMKFGGTSVGSEDAFRQVCEIVEQARSEWPRMVV
                     VTSAMAGVTNLLLESASKAVEGNTEYVHQIAQRIRTQHWEILAALLPDLARQAQVKQE
                     INHLIGEFTNLCQAIQVLGEATPRALDAVASLGERLSVRVLAAALDSRGMPAQFVEAT
                     QLIITDNQFQSAHPDFELTRQAIGRVLEPILGQGKIPVITGFIAATPDGITITLGRGG
                     SDYTAAILGAHLPADDVWIWTDVDGVMTADPRAVPGAHTISHLSYREIAELAYFGAKV
                     VHPKTIRPIIEAGIGLRVCNTFNPNHPGTRVVADHPLSQKNNSISNETIKAVTTIRGQ
                     SLITVEGRGMLGVPGVAARTFGAVAATGTSVPLITQASSEQSICFAVPIDTVDSVIAS
                     LESAFANELNRGDIDRIWATEEVTIITIVGSGMRNTPGIAGQIFSALGDRGVNVIAIA
                     QGSSEVSISLVVDAKDADAGVRSVHDLIDRIIRP"
     gene            2500..2571
                     /locus_tag="AC16CB_08625"
     tRNA            2500..2571
                     /locus_tag="AC16CB_08625"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2532..2534,aa:Met,seq:cat)
     gene            2626..2699
                     /locus_tag="AC16CB_08630"
     tRNA            2626..2699
                     /locus_tag="AC16CB_08630"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2660..2662,aa:Asp,seq:gtc)
     gene            complement(2824..3414)
                     /locus_tag="AC16CB_08635"
     CDS             complement(2824..3414)
                     /locus_tag="AC16CB_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342574.1"
                     /translation="MSSSNLVRWSGMATIVGAILIAILELMEFILFGSLTESQVAATG
                     GWVFVQAIYLIAMLAVLLGLIGLYTRQVEQSGNFGVISFLVAFIGTVLFAGAQWSTAF
                     IGPMLAAEAPGIMDAEPTGSILVGYLLSVILFSLGWLLFGWASLRSSVFPRGAALLVA
                     VGGVLFFIMGSLEWPAATLALDAGLAWMGYTLWSAK"
     gene            complement(4182..4979)
                     /gene="otsB"
                     /locus_tag="AC16CB_08640"
     CDS             complement(4182..4979)
                     /gene="otsB"
                     /locus_tag="AC16CB_08640"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HIR3342575.1"
                     /translation="MKTQPAQQALTRLVNFERLWLFLDYDGTLAEFAPSPDIVIPDPE
                     LLDLLTRLANKPDLRLAVISGRRLQHIRKLVPIPEIWLAGTYGIELLSPSGGVRSQLN
                     FESIRPALEKLKPKWETLIASQGGFYIEDKGWSLALHARFASDENAEKILAEASRLAR
                     QLIQKSKLRLLGGHKFLEICPTTADKGKAVQKILAYDAFSGALPIFIGDDDKDEGAFA
                     VVKAHHGIAILVATESRKTQADYYLDSPYSVRGWLQNLLQFRSRGIN"
     gene            complement(4987..6234)
                     /locus_tag="AC16CB_08645"
     CDS             complement(4987..6234)
                     /locus_tag="AC16CB_08645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3342576.1"
                     /translation="MYKPRIGILHYTAPPVVGGVEAVIQAQASLFNRMEYPVSVVAGR
                     GEASILPPGTSLVLLPLLDSQHEEILHINAYLENGDVHPGFDSMVEKIAQALQPVVEE
                     VDHMIIHNVFTKHFNLALTVALDQLIEAGSIRHAIVWCHDISWTSPSSRSKVFPGYPW
                     DILRTYRSDVTYVTVSAHRQKALAKLFECSLHKIHLIYNGVEPSSLLGLSPEGLRLAD
                     RLNLFGSSLILLMPVRVTRAKNIEYALHVVTALKQEVSTKLVLTGPPDPHDPENMRYF
                     HSLLRLRQKLGVESEARFIFEEGYQPDEPYLITERVVGDLFRLCDVVFMPSHREGFGM
                     PVIEAGLSGVPVICTDIPAAQEIGQKEVTIFSKEQSPSDVARVIIEIVEANPVSRFRR
                     RVRQNYTWEALFQRQIEPILKGQ"
     gene            complement(6247..7731)
                     /locus_tag="AC16CB_08650"
     CDS             complement(6247..7731)
                     /locus_tag="AC16CB_08650"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase
                     (UDP-forming)"
                     /protein_id="HIR3342577.1"
                     /translation="MEPGQNDNKAQQRPIIIASNRGPVSLNRNEAGGYEIQRGSGGLV
                     TALTGLASLIDATWISCALSADDTEWGEGEVPFNDKGDSIFVKFLTPEESAFDGYYNV
                     IANPLLWFLQHSMWDVPRAPVINRATWRAWEEGYEQVNREFALEIANQVNRVSQPVLI
                     MLQDYHLYLAARYLRETVPSQAITILHFIHIPWPGPEYWGILPPTMRQAILEGLCAAD
                     LLGFQTREDALNFMRTCESYQPKASVKYKRGQIWYRNHATHVRDFPISIDVEALKAMA
                     NSPEVQQHHAEIQDIVGGRKLILRIDRIEPSKNIIRGFQAFEEMLELYPEHRDQIIFL
                     ALLVPSRLELEEYKNYLDDLMGTAGQINAHYGSSQWEPVRLLVGDNYPRAVAALQQYD
                     LLLVNAIADGMNLVAKEGPIVNQKDGVLILSDRAGARQQLESGAIVISPCDVYATAEA
                     MHQALVMPQGQRYQYAERLRWMIEREDITAWLEHQLEAVDELNL"
     gene            complement(7759..9552)
                     /locus_tag="AC16CB_08655"
     CDS             complement(7759..9552)
                     /locus_tag="AC16CB_08655"
                     /inference="COORDINATES: protein motif:HMM:NF046269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HIR3342578.1"
                     /translation="MTNKLASPKLSMLRNAIDFTRVPFSDRGSRLMVFQAAYQSHFYI
                     KLAERLTSVEPGIETYLRRPPLIHNLCLIDADGELLEFDITTFPHAIYMSTRIGDFAM
                     VFGDNHTIAFGLPPEVTAGIQFEVSPHLWKVLEFGGEIKSVRDLSYATNGTPVHNKIS
                     LGNTGHRVELIVEADSDCTLALMIGDQRSPVAGIEPFSTLYDSAEKRWQDWFRNVPPV
                     ADKYRRAYAYAWWIMANNLINPGGIIHYEAMMPSKASYVGLWLWDSAMHALAYRHIDP
                     ELARNQIRAILAYQLPDGMLPDAIYDEGIISEIDHPIKAEVTKPPILAWAALKLHETD
                     PNLEFLQEIYVPLVRWNAWWFSMNDDDVDGLAQYNHPYSSGLDDNPLWDYGMPVESPE
                     LNSYLCVQMGSLAIMAELLGMNAEAAMWRRRAEAIVRRMIQDFWDEQEGVFRATHNDQ
                     IIPVLTPFNLYPLWTGQLPENYRTRLLDHLTNPDEFWGEYILPSVARSDPHYDPDTMW
                     RGPVWSNINYFFIEALNQIGEKDLADELRDKTLEMILAHPGMYEYYNSETGQPPARSA
                     EAFGWTAAVFIDLAISASQEQQAGSQQSIVD"
BASE COUNT         2417 a         2263 c         2436 g         2529 t
ORIGIN      
        1 gccgattttg gcttggtagg cgttgaggtg cgctcgatac ctcaggatga tctaaatatt
       61 ttgcgggaag aactccagtc atattgtgac tcacaaggac tggagcttca gatacccgtt
      121 atggaaaatg gggtggtgtg cgatccacag aaccagtatt taaaatcttt gctgcaagct
      181 gtagagaacc tatcgggcga aaaacctcgc attgggaaaa agctgcctgg caccagcgca
      241 cgatttgcac ctgggggaca gggtgtggtt tgggggcaaa gtggtttggg accacatgct
      301 gcgaatgaac gtcactttat ccctagcatc gagccatatt accgggcgct aggtgagttt
      361 ggaaggctcc tgaagggatg agtattgcct ttttactcaa aggcttagtg atcggattct
      421 cgattgccgc gccggttggt ccgattgggg tgttatgtat taagcggaca ctgacgaatg
      481 ggcgcatgtc tggattggtt tctggcttag gagctgccac agccgatgct ttgtatggtt
      541 ccatcgctgg tttcgggctt acattgattt ccgcgttttt gcttgaccaa caaacctgga
      601 tacgcctggt tgggggggca ttcctgtgtt atctaggttt gaaaactttc atatctaaac
      661 cagcagaaca ggatgtcaga gcgcatggtg gtaacctgat cagcgcttat ctcagtacat
      721 ttgtgcttac gctgaccaat ccattgacca tcctgtcctt tgcagcaata ttcgcaggat
      781 tgggcctggg cgctacggat ggcgattttg catcggcagg tattttagtt agtggtgtat
      841 ttttaggctc tgctgcctgg tggctattgc tgagtggtgg cgtcagtttg ctgcggcaga
      901 ggcttgacca gcaggttatg gcttgggtaa acagggcctc gggtgtgatc attttgggat
      961 ttggagtggc agccttatgg aaattgatct gaggaatatg tcttatacgg gaggtagtga
     1021 tgcaagagag gcgtgattcc cgtacgctag tgatgaaatt tggtgggact tctgtaggta
     1081 gtgaggatgc attccgccaa gtatgtgaga ttgttgagca ggcgcggtca gagtggccgc
     1141 ggatggtggt ggttacctca gccatggcag gtgtcactaa cttattgctg gaaagtgcct
     1201 cgaaagcggt tgagggcaac accgagtacg tccatcaaat tgcccagcga attcgcacgc
     1261 agcactggga aattttagca gccctcttgc ccgatcttgc tcggcaggcg caggtaaaac
     1321 aagagatcaa ccatcttatc ggcgagttca cgaacctttg tcaggcaatc caggtgttgg
     1381 gagaggcgac ccctcgcgcg ctggatgcgg tggcttcgct tggtgagcgc ctgagcgttc
     1441 gcgtattggc cgcagctctg gatagcaggg ggatgccagc tcaatttgta gaggctactc
     1501 agctgatcat tacagataac caatttcagt cagcccatcc agattttgaa ttgacaagac
     1561 aggcaatcgg cagggttctt gaacccatct tgggacaggg aaaaatcccg gttatcacgg
     1621 gatttatcgc cgcaacgccc gatgggatta cgatcactct tggacgaggt gggagtgact
     1681 acactgccgc aattttggga gcacacttgc cagctgacga tgtctggatt tggacggatg
     1741 tggatggcgt aatgaccgct gatcctcgtg ctgttcctgg ggcacatacc atctcacatc
     1801 tttcgtatcg ggaaattgcg gaattagcct attttggggc aaaagtggtt catccgaaga
     1861 caattcgccc aatcattgag gctgggattg ggttgcgtgt ttgtaatacc ttcaacccaa
     1921 atcatcctgg cacacgagtt gtagccgatc atcctttgag tcaaaagaac aacagcattt
     1981 cgaatgaaac gatcaaagca gttaccacca tacgcgggca aagcctgatc acagtggagg
     2041 ggagggggat gttaggtgtt ccaggtgtcg cagcgcgcac atttggagct gtggctgcaa
     2101 caggtactag cgttccgttg attactcagg catcttcaga gcaatcgatt tgctttgcag
     2161 tgccgattga tacagtcgat agtgtgattg cttctctgga atcagccttt gcgaatgagt
     2221 tgaatagagg ggatatcgac cgaatttggg cgactgagga agtcaccatc atcacgatag
     2281 tgggctcggg gatgcgcaat acgcccggaa tagcgggaca aatcttcagc gcattaggtg
     2341 accgtggcgt caatgtgatc gctattgccc aagggtcgtc ggaagtatct atcagcctgg
     2401 tagtggatgc aaaggacgct gacgcgggtg tgcgctcggt gcatgatctg attgacagaa
     2461 taattagacc gtagtaatat ttagcccgcc tcaatgactg gcgaggtagc tcaatggcag
     2521 agcaggggac tcataagccc ttggttggcg gttcaaatcc gcccctcgcc actgagccgt
     2581 agcgcttgac ggagggtttg aaatcgatta aactacggct tgcttggtcc cgtggtgtag
     2641 cggcctaaca tgcggccctg tcaaggccga gatcgcgggt tcgaatcccg tcgggaccgc
     2701 agaatgaaat cgcatagtga aaactatgcg atttcattat ctatatccta ttcacacatc
     2761 cctgacgaaa agatcatcgt cagtatgtaa gcgaagagaa aagcttaatt gcttacaact
     2821 gttttacttt gcggaccaga gcgtataccc catccaggcc aggcctgcgt ccaaggctaa
     2881 agtcgcagcg ggccattcaa gtgatcccat gataaagaat agcacacctc caactgcaac
     2941 cagcagcgct gcgccgcgcg gaaaaacgct ggatcgcagc gaggcccagc caaatagaag
     3001 ccagccaagc gaaaacaata ttacggaaag caaatatccc acaagtatgg acccggtagg
     3061 ctcggcgtcc atgatccccg gagcttcagc ggccaacatc ggccctataa aggctgtgct
     3121 ccattgcgcg ccagcaaaca gcacagtgcc gataaatgcc accaagaagg agattacccc
     3181 aaaattccct gactgttcta cctggcgtgt atacagacca attaaaccca gtagcacggc
     3241 caacatagca atgagatata tcgcttgtac gaaaacccag ccacctgttg ccgccacctg
     3301 actttctgtt agactcccaa acaggataaa ttccatcaat tcgaggatgg caattagtat
     3361 ggcacccaca attgtggcca tgccactcca acgcacgaga tttgaagacg acattttaat
     3421 ttcctccttt gtgatatcct gaatttagtt cttctgaccc agtacatagc gcaaaacgat
     3481 aacctacaat tcgttttcgc atcgccaacc aaggcttgca gtgtattcgt atttacttag
     3541 aaggggtgat tattcgcttg tttaacagct tcctttctgc cgacgacgat cattttgtgg
     3601 actccacgaa agtgcgtcaa gctttaactt gggaaagagt aataaatgac cctttgtgat
     3661 tgatcacaaa gggtcatgcc accgcagtgc agaaagcagg tgaaatcagc cagctatata
     3721 aatcttgaag cagataccgc caagaaaaaa taccctcgca ttttattggg ggatgaaatt
     3781 gggtttgtgc cagtcttaga aggggctcgc ctagttgttt tacaacatag cgcattagtt
     3841 agcacctctc atttttatgt tccgttcaaa tattatcccc ctggttaccg ttggcaggtg
     3901 aagttccgac actagcctca gtatgctatc ggactttgtg gcatcttaat tgccactaat
     3961 aatacactaa tttggctcac ttagtcaata tttttttaat aaattgatgt gataggcatt
     4021 tgtggttttc tattttcatc ttgatatagt tgatactggg agagcgtgtc gctgagagtc
     4081 actgctcttt caggaagaac tggtggatta gactagaaac ctgaaatgag tagtaaactg
     4141 gattaagtta aacaagaccg tttattgccg ggaggcgccc gctaattaat accccttgag
     4201 cgaaattgca gcaaattctg tagccatcct cttactgaat aaggtgaatc cagataataa
     4261 tctgcctggg ttttacggga ttcggtagca actagaatgg cgatcccatg gtgagctttt
     4321 accacggcga acgctccttc gtctttgtcg tcatccccta taaagattgg taatgccccg
     4381 gagaatgcat catatgctag gatcttctgg actgctttgc ccttatcggc agtggtgggg
     4441 catatctcga gaaatttatg ccctcctaac agccgtaact tcgatttttg aatcaactga
     4501 cgggcaagac gactggcttc agccaggatt ttttctgcgt tttcatcgct ggcaaatcga
     4561 gcgtggagtg ctaaagacca tcccttatct tcaatgtaaa agcctccttg gctagcaatt
     4621 agtgtttccc acttaggttt taatttttca agcgctgggc gaattgattc aaaattcaat
     4681 tgggacctaa ctcccccgga tgggctaagg agttcaatac cgtaagtgcc tgctaaccag
     4741 atttctggta tcggcacgag tttacgaata tgttgaagtc ttctaccgct gatcactgcc
     4801 aaacgcaggt cgggcttatt tgccaatctt gttagcaaat cgagaagctc tggatcggga
     4861 atcacgatat ctggcgaagg agcgaattca gccagagtgc cgtcatagtc caagaaaagc
     4921 caaagtcgtt cgaaattaac cagtcgggtc aatgcttgct gagctggttg agtcttcata
     4981 agtctttcac tgacccttca aaattggttc aatttgacgt tggaaaagcg cttcccaagt
     5041 atagttttgc cttacccgcc ttcgaaagcg cgacactgga tttgcttcca caatctcgat
     5101 gattaccctt gctacatcgc tgggtgattg ttctttggaa aagatggtta cttccttttg
     5161 accgatttct tgagccgcag gtatatcagt acatattaca ggcacaccac ttaaaccagc
     5221 ttctataaca ggcatgccaa atccttcgcg atggctaggc ataaagacaa catcgcataa
     5281 gcgaaataaa tcacctacca cgcgttcggt aatcaggtag ggttcgtcag gttgatatcc
     5341 ttcttcaaat atgaagcgcg cttcgctttc tacgccgagt ttctgtcgta gcctcaacaa
     5401 tgagtggaaa tatctcatat tctcagggtc gtgcggatcc ggcgggcctg tcagtaccag
     5461 tttcgtactc acttcttgct ttagcgccgt aaccacatgc agtgcatact cgatgttttt
     5521 tgcgcgtgtt accctgacag gcattaaaag tatcaggctt gagccaaata agttcaagcg
     5581 atcagccaat cgaagtcctt ccggggatag accgagcagg ctggaaggtt caacgccatt
     5641 gtagatcagg tgaattttgt gcaggctgca ttcgaacagc tttgccaaag ccttctggcg
     5701 gtgtgcagaa actgttacat aggttacatc tgaacggtaa gtccgaagga tatcccatgg
     5761 atatccaggg aatacttttg agcgcgagct tgggctcgtc caggagatat catggcacca
     5821 aacaatggcg tgacgaatgg agccagcctc gatcagctgg tctagcgcaa cggtcaaagc
     5881 caggttgaaa tgtttggtga aaacattgtg gatgatcatg tgatcaacct cttctaccac
     5941 tggttgaaga gcttgagcta tcttttcaac cattgagtca aaacctgggt gcacatcacc
     6001 gttttctagg taagcattaa tgtgtaatat ttcttcgtgt tggctgtcaa gcaacggaag
     6061 gagcactaac gaggtgccgg gtggcaatat ggaggcctca ccgcgccctg cgaccacaga
     6121 gaccggatac tccattcgat taaaaaggct tgcttgtgct tggatcactg cctccacacc
     6181 gccgacgaca ggtggagcag tgtaatgtaa aatcccaata cgtggtttat acataagacg
     6241 ctcgtactat agattaagct catccactgc ctcaagctga tgttctagcc aagcagtgat
     6301 atcctcacgt tcgatcatcc agcgcaaacg ctcagcatac tggtagcgtt gcccctgcgg
     6361 cataacaagg gcttggtgca ttgcttctgc cgtagcgtac acatcgcatg gagagatgac
     6421 gatagcacct gactcaagct gctgtcgggc gccggctcta tctgaaagga tcagtactcc
     6481 atctttctga ttgacgattg gtccttcttt tgcgaccagg ttcataccat ctgctatggc
     6541 attaaccaat aagaggtcgt actgttgtaa ggcagccact gctcgcgggt agttgtcacc
     6601 taccagcaga cgaactggtt cccactggct gctaccgtaa tgcgcattaa tctgaccggc
     6661 ggtgcccatt aaatcatcca ggtaattttt atattcctct aactccaagc gcgagggtac
     6721 cagcaaggct agaaaaataa tctgatcacg atgttcggga tacaattcaa gcatctcctc
     6781 aaatgcctga aagccgcgga tgatgttttt gctaggttca atgcgatcaa tgcgcaggat
     6841 cagtttgcgt ccaccaacaa tgtcctgtat ctcagcgtga tgttgctgaa cctcaggtga
     6901 attggccatt gccttcaaag cctcaacgtc gatggaaatg ggaaaatcgc gcacatgagt
     6961 ggcatgatta cggtaccaga tttgaccacg cttgtatttt acgctggctt ttggttggta
     7021 agattcacag gtgcgcatga agtttaaggc atcctcgcgt gtctgaaagc caagtaaatc
     7081 agctgcacac agcccttcta agatggcctg gcgcatggtt ggtggcaata ttccccagta
     7141 ctctgggcca ggccatggaa tatggataaa atgcaagatc gttattgctt ggcttgggac
     7201 ggtctctcgc aggtatcgag ctgcgaggta gagatgataa tcttgcaaca taatcaatac
     7261 tggttgagaa acgcgattga cctgattggc aatctccagg gcaaactctc gattgacctg
     7321 ctcataacct tcttcccagg cccgccatgt ggcgcggttg atcaccggtg cgcgcggaac
     7381 atcccacatg gaatgctgta gaaaccagag taatggattg gcgatcacat tgtaatagcc
     7441 atcaaaggcg gattcttcgg gtgtcagaaa ttttacgaaa attgaatcgc ctttatcatt
     7501 aaatggtact tcaccttctc cccattctgt gtcatcagcg ctcaaggcgc aggaaatcca
     7561 ggtggcatcg ataaggctag ctaaacctgt tagagctgtg accagaccac cgctgccgcg
     7621 ctgaatctcg taacccccag cttcattccg gtttaaagat accggtccgc ggttggatgc
     7681 gataatgatc ggacgttgtt gcgccttgtt atcgttttga cctggttcca taatcacaac
     7741 tctcctttac cagggctatc aatcaactat agactgttgg gagccggctt gttgctcctg
     7801 actggcggaa attgctaagt cgatgaacac agctgcggtc cagccgaaag cttcggcgga
     7861 acgcgcggga ggctgcccgg tttcggaatt gtagtattca tacatgccag gatgagccag
     7921 gatcatttcc aaagttttgt cccttagttc atccgctaaa tccttctcac caatttgatt
     7981 taaggcttca ataaaaaaat agttgatgtt cgaccatact ggcccacgcc acatcgtatc
     8041 agggtcataa tgcggatcac ttcgtgcaac ggatgggagg atatattccc cccagaattc
     8101 atcagggttg gtgaggtgat ccaatagtcg agtgcggtaa ttttcgggta gctgcccggt
     8161 ccacaaaggg taaagattga agggtgtcaa aacaggaatg atttggtcgt tgtgcgttgc
     8221 cctaaagaca ccttcttgtt cgtcccaaaa atcttggatc atgcgtcgca cgatcgcctc
     8281 agcccgcctg cgccacatcg cagcttcggc gttcatgccc agtaattcgg ccataattgc
     8341 caacgagccc atttggacgc aaaggtagct attcagttct ggtgattcta ctggcatacc
     8401 ataatcccat agtggattgt catctagacc ggatgagtaa gggtggttgt actgtgctaa
     8461 accgtcgaca tcatcatcat tcatgctgaa ccaccaggca ttccagcgta ccaatggtac
     8521 atatatctct tgtaagaatt caagattagg gtcagtttcg tgtagtttta aggctgccca
     8581 agctaaaatg ggaggtttgg tgacttcagc cttgattggg tggtcaatct cggaaataat
     8641 gccttcatca tatattgcat ctggcagcat cccatcagga agctggtaag ccagaatagc
     8701 acgaatctgg ttgcgggcca gctcaggatc gatgtgacgg taggccagcg cgtgcatggc
     8761 actatcccac agccataatc ctacataact tgccttagat ggcatcatgg cttcataatg
     8821 tatgatacct ccgggattga tcaagttatt tgccatgatc caccaggcat aggcgtaggc
     8881 tctgcggtat ttatcagcca ccggtggcac attgcgaaac caatcttgcc agcgtttctc
     8941 ggccgaatcg tacaaggttg agaacggttc tataccagct acgggagatc gttgatcgcc
     9001 aatcatcaag gcaagtgtgc aatcgctatc cgcctccacg atcaactcaa ctcgatggcc
     9061 ggtgttccca agagagatct tattgtgtac aggcgtgcca ttggtggcgt aggataggtc
     9121 acgcacactt ttgatttcgc caccgaactc tagcaccttc caaagatgag gtgagacttc
     9181 gaattgaatc cctgcggtga cttcgggtgg caatccgaag gcaattgtat ggttgtctcc
     9241 aaataccatg gcgaaatcac cgatccgcgt gctcatatat atcgcatgtg gaaacgtggt
     9301 gatgtcaaac tccagcagct caccatcggc atcgatcaga catagattgt gaattaatgg
     9361 tgggcggcgt aaataagttt cgataccggg ttcaacactg gtaagccgtt ctgcaagctt
     9421 gatgtaaaaa tggctctgat aggcagcctg aaataccatc aggcgcgagc ctctatcgct
     9481 gaatggcacg cgcgtgaaat caatcgcgtt tcgtaacata gaaagttttg gtgaagctag
     9541 cttatttgtc atatggttat tataggatat ttattatcaa tagcagtgtt cctcgttgtg
     9601 gaatgctatg cttgatgatg actttagccc cgcactcagt cctaa
//
LOCUS       DBNGSX010000284         5860 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_577099, whole genome shotgun sequence.
ACCESSION   DBNGSX010000284 DBNGSX010000000
VERSION     DBNGSX010000284.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_577099"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="AC16CB_08660"
     CDS             complement(<1..328)
                     /locus_tag="AC16CB_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3342579.1"
                     /translation="MTKQRILLVDDHEVVRLGLKSLLDRHPNFEVVAEASTAREAIER
                     VGTHNPDVVVMDIRLPGGSGIEACEEIAEKHPNTKVIMLTSYAEDEMLFSAIRAGASG
                     YVLKQIG"
     gene            906..2618
                     /locus_tag="AC16CB_08665"
     CDS             906..2618
                     /locus_tag="AC16CB_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342580.1"
                     /translation="MKRTLKWLCILSLFVLLTASLTAASGAGDSPRFVRHKLVEGIGM
                     RGSNGINFDSQNRLYIASVFSAGIIVMDPQNGKILQSYGPDDGVLGPDDLAFGPDGTL
                     YWTDLSQGMVGRMTPDGVSSAQMVTPGVNPITFSADGRLFVALDFFGDGLYELDPMFV
                     DSPKPIILATPANPMPLGFLNGMDFGPDGRLYGPIYTQGKIISLDVDSCDPVPTDTPW
                     QDCDIATVADGFGVPAAAKFDSHGNLFVVDQVGLLYQVDVATGEKHVFAELSPMLDNL
                     AFDTQGHLYISNAADGYILEMLPSGKPRMVSKDGQMSAGSLALLPEPRGGDRLFTADL
                     WSLRQFNALTGRSLHTEYQVPPLSSIIAPLTVATAGSNLVLSSWVSSMVQVWDPQTNQ
                     QVASYSFPVPLNAISFMDDVVVADLALGGVYWASNHAPLISAPYVVIPSGLVAIQDDL
                     YVCDWATGILWQLYEDGLPLSSPRMVTGGLNLPEGMAAYTDGSLLVVETGRSQITRID
                     PQTGEKEPLIQDLDVGVILDGFPPTFIFSGIAVGSDGAIYASSDVENVIYRFMPVPRR
                     PSTP"
     gene            complement(3337..4383)
                     /locus_tag="AC16CB_08670"
     CDS             complement(3337..4383)
                     /locus_tag="AC16CB_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342581.1"
                     /translation="MKIKYALIGTLLLVLALALAACAGAAGPEGPAGPEGPAGPPGPA
                     GPPGPAGPAGAAGAAAEIGELTCTQCHNDTTLIVSKQAQFREVSVHGTGEAFVRGEST
                     NCAGCHGSEGAKARIDAGLPPHDPSVEGIVNVSPYNCRTCHQIHTTYTRDDFALTGNE
                     QPAPMEMTGGTFDGGQGNLCANCHQIRNELPVAADGNIDLGENTRFGTHYGVEAQMML
                     GVGGLGVTGNPSPHYLMVEDTCVGCHMGDERSHTYLPEVERCQACHADAEDFNMNGVQ
                     TEIEEMLAEVHDKLIASGIMNEEGRSIPGVYPEGVASAMWNYKLVEYDQSKGVHNSAY
                     AKALLEAALAGLGQ"
     gene            complement(5048..5770)
                     /locus_tag="AC16CB_08675"
     CDS             complement(5048..5770)
                     /locus_tag="AC16CB_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3342582.1"
                     /translation="MENSFRDRRILVVDDEERMVRFIRLNLEHDGFQVTEAFRGSQAI
                     DRLRSTLPDLVLLDVMMPDIDGFEVLRVIREVSTVPVIMITAKGEEEDRIRGLELGAD
                     DYITKPFSPREMVSRVKAVLRRTETSSASTHGLIDVDERLKLDFDRREVWVEGQLVKL
                     RPTEYRLLYHLVQNAGWVITHDQLLAKVWGYEYRDEPHYVRLYINYLRQKIEKDPANP
                     QYILTERGVGYRFVDFRRQTPG"
BASE COUNT         1363 a         1554 c         1392 g         1551 t
ORIGIN      
        1 ccccgatctg cttgagcaca tatccagacg caccagcgcg gatcgccgag aaaagcatct
       61 catcttcggc gtatgaagtt agcatgatca ccttggtatt tgggtgcttt tccgcgatct
      121 cttcgcacgc ctctatcccc gatccacctg gcaggcgaat atccatcacg acaacatcag
      181 ggttatgagt acctacccgc tcgatagcct cacgcgctgt gctggcctct gcaacgactt
      241 caaaatttgg gtggcgatcg agcagggatt tcaaccccaa acgcacgact tcatggtcgt
      301 ctaccaataa aatccgttgt ttagtcatct atatagaata tcctgtgaaa aatgcacata
      361 ttatgcgaca ttttgcatat tgacccccat atatagaggg tgtgtaattt gttatcttgt
      421 gctagtatat caattttaca ccatttgcgt aggtaggcga ctgcctcata aatgattttc
      481 ccagattcga aaatctcgca gattcacagc cggacggttc ggggttgcaa atcctggctg
      541 gaatgtatac cacattgata gcggcgggtt ctgggaagtc ttgttagtct gggagagaat
      601 ccacacacct ctgtctcaac ttatttatga aatttatcga ccgcttgttg tgcaagaacg
      661 tgaaaaactt gactctatct gtgatatttt atacaatatc aataatctac cgtctatcac
      721 agtcacaacc tgggcggtaa tctgacctca ttccagctac ttagccatcg taattcgtca
      781 tcctgccgcc ggttggtcct cggcggttgt tttgtttttt caaaggaggg gatttgcgac
      841 aattttctgc ctatcacacc ctgcgataga aaattcttac tttccaaact taatggaggc
      901 aactcatgaa acgcaccttg aaatggctct gcattctcag cctgtttgtc ctgctcacgg
      961 cctctctcac cgctgcatcc ggcgcaggtg actcgccgag gtttgtccgc cacaaactgg
     1021 ttgaaggcat cggcatgcgc ggctcgaatg gcatcaattt tgactcccag aaccgcctgt
     1081 atatcgccag tgtcttctct gctgggatca ttgttatgga tccccagaac ggaaaaatac
     1141 tgcagtccta tggtcctgat gatggcgttc tcgggccaga tgacctcgct ttcggtccag
     1201 acggtacact ctattggacc gacctcagcc agggcatggt cgggcgcatg accccagatg
     1261 gggtctccag tgctcagatg gtaaccccgg gcgtcaatcc catcaccttt tcggcagacg
     1321 ggcgcctgtt tgtagccctg gacttctttg gcgatggcct ttacgagctg gatcctatgt
     1381 ttgttgactc gccaaaaccg attattctcg ccactcctgc caatcccatg ccactcggct
     1441 tcctgaacgg tatggatttc ggcccggatg gacgcctgta tgggccaatc tacacccagg
     1501 ggaagattat cagcctggat gtcgattcct gcgatcctgt gcctaccgat accccgtggc
     1561 aggactgcga cattgccacc gtggcggatg gcttcggtgt ccctgcagcc gctaagttcg
     1621 actcacacgg caacctgttc gtcgtcgacc aggttggcct gctttaccag gtggatgtgg
     1681 caactggcga aaagcatgtg ttcgccgagc tgtcgcccat gttagacaac ctggcatttg
     1741 atactcaagg ccacctgtac atctcgaacg ctgctgatgg gtatatcctg gagatgctgc
     1801 ccagcggtaa gcctcgtatg gtcagcaaag acgggcagat gagtgctggc tcattggcgc
     1861 tcttaccaga gccgcgtggc ggcgatagac tctttaccgc agatctttgg tccttgcgcc
     1921 agttcaacgc cctcaccggc cgatcactgc acacagaata tcaggtaccg cctctatctt
     1981 ccattatcgc cccgctcacc gtagccactg ccggttcgaa cttggtgctc tcctcctggg
     2041 tatcgagcat ggtacaggtt tgggatccac aaacgaacca gcaagtggct tcttacagct
     2101 tcccagtacc gctcaacgcc atcagcttca tggacgatgt tgtcgttgca gatctggcct
     2161 tgggtggcgt atactgggca agcaatcacg ctccactgat cagcgcacct tatgtagtta
     2221 taccctctgg attagttgcc atccaggatg atctctacgt ctgcgactgg gccaccggta
     2281 tactttggca gctctacgag gatggcctcc cgcttagctc gccgcgtatg gtcaccggtg
     2341 gcttgaattt accagagggc atggccgcct atactgatgg tagcctgctg gtcgttgaga
     2401 ctggccgcag ccagatcacc cgtattgacc cacagaccgg cgagaaggaa ccgcttatcc
     2461 aggatctgga tgtaggggta atattggatg gatttccgcc tacctttata ttcagcggca
     2521 tcgctgtagg atcagatgga gcgatatacg cctcctcaga cgtagaaaac gtcatatacc
     2581 gcttcatgcc cgttccgagg agacccagca ccccgtagtt tcactcccag ccacgccaca
     2641 cgagaaaagt tcacgaatag ttagatgcct tgagaaggtg cttcccttcg aaaggcatct
     2701 ttagtaccca atttccatga tgtatcgtat acctcgaggt aaaaactgat acatcactgt
     2761 ttagtcatct aaaaagttat cccgtgaaaa atgcaaatta tgcgacgttt tgcattttgc
     2821 ctcaatctat gagggtgtgt ggattggctc aagtgcttgt agattaattt ttacttcaac
     2881 tctgtagaat agcctcggtg aatctaaacc gcactttgta caactccgag gatttatcaa
     2941 attttcacca ttctgggtag gtaggcgact accaaatagc tgattttgcc agtcttgcaa
     3001 tgcttaaaag cctcattcaa gacttccctc agctctggat gaaactcaat agtcgcctca
     3061 tacttatccc tcaaattacc cgactccttt tcaatataca gcctatctca catagagcag
     3121 taccatacta gaagcgtctt catgcgaggt taaaacccgg gcgctcttga tctctaaaca
     3181 gtcacagaat gacaactatt cagcgaaaag atcacaaagc tttatctaaa tttttccgtt
     3241 gattccgtct ccataaaaaa caactgggcg agctcatgat ttggagctcg cccagacgta
     3301 ttaggttaat tttcgaacag cagcttgatc agaattttac tgccctaagc cggctagtgc
     3361 cgcttcgagt agagccttgg cataggccga gttatggacg cccttactct gatcatactc
     3421 cacgagcttg taattccaca tcgccgatgc gactccttca gggtacactc cggggatcga
     3481 ccggccttcc tcgttcatga tgccgcttgc aatcaacttg tcatgaacct cagcgagcat
     3541 ttcctcgatc tcggtctgca cgccattcat gttgaaatcc tcagcatccg catggcaggc
     3601 ctgacaacgc tccacctcgg ggaggtaggt gtggctgcgt tcatctccca tgtggcagcc
     3661 tacacatgta tcttcgacca tcaagtagtg agggcttgga tttccagtca cacccaatcc
     3721 acctacgcca agcatcatct gggcttcaac accgtagtga gttccgaatc gggtgttctc
     3781 accaaggtca atgttcccgt cagccgcaac gggaagttcg ttgcggatct ggtggcagtt
     3841 tgcgcacagg ttgccttggc cgccatcaaa ggtcccacca gtcatctcca ttggcgccgg
     3901 ctgctcatta ccggtcagcg caaagtcatc gcgggtgtag gtggtgtgga tctggtggca
     3961 ggtgcgacag ttatacggcg agacgttcac aatgccctcc accgatgggt catgtggagg
     4021 caacccggca tcgatgcggg ctttggcgcc ttcgctgccg tgacagccgg cacagttggt
     4081 gctttcacct ctcacaaagg cctctccggt tccgtgaaca gaaacttcgc ggaactgcgc
     4141 ttgcttggag acgatcaaag tcgtgtcatt gtggcactgt gtgcaggtca actcgcctat
     4201 ttctgcggct gcgccagcgg ctccagcggg accagcaggt ccgggaggac cggcagggcc
     4261 aggaggtcca gcgggacctt cgggtccagc aggtccttcc ggtccggctg caccggcaca
     4321 ggcagccagt gccagtgcta gcaccaggag caaggtccca atcaaggcat atttaatctt
     4381 catttatatt ctcctctcaa gaatattgaa actctgcgtt cagaaaggta tccaaattgg
     4441 tgtcaattta gtgtttagcc tatgattgtc cgttcacctc ctttggcaat atagtccgaa
     4501 atacctcacc tgtagaaaaa gggggccaac agaatttggt caaacatagt gaattataca
     4561 ctatttatga gaaaataatg agtgaatatc aatcgacttt ccgatctgat atgtgattgg
     4621 ctgattggat tctcatagca cggtgaaagg taaacgggta atcacatgag caaaaagccg
     4681 attagatgct cggttcataa agtgactcgg gcaaattgcc ctggaaatga gtttcttcat
     4741 tattgccatt gccgcttgtg atcctgtgca aaggatggat gaacaatgag gtaatctgac
     4801 tagaacagcc ctgttccttg taatgaatgc tagtgagcta tctattaccg ggtttgctct
     4861 gagagtgtca aatgtcgtga cgagcagaga tgtttaggat tcacttgatt accatgtata
     4921 taatgcgcaa aaattgttgg gcaagcctat gtcttgtagc ttgcccaaca atttttgcat
     4981 tttgtgcggt tgcgttatct tattttagcc ctgattatgg ggtattgctc tagctatctt
     5041 atgaattcta tcccggtgtt tggcggcgaa aatcgacaaa gcgataacct acgccacgct
     5101 cggtgaggat atattgcggg ttggccggat ctttttcgat cttttgccgc agatagttaa
     5161 tataaaggcg cacataatgc ggctcatctc ggtattcgta accccaaact ttggccagaa
     5221 gttggtcatg ggtgatcacc cagccggcgt tttgcaccag gtggtatagc agccgatatt
     5281 cggttggtcg cagtttgacc agttgccctt caacccaaac ctcgcgccgg tcaaaatcta
     5341 atttcaaccg ctcatccaca tcgatcagtc cgtgtgtgct ggctgaggat gtttcggtgc
     5401 ggcggaggac agccttcaca cgactgacca tttcgcgagg gctgaaaggc ttggtaatat
     5461 aatcatctgc tcccagttct aaaccgcgta ttcggtcttc ttcttcccct ttggccgtaa
     5521 tcatgatcac cggcacagtg ctgacctcgc ggattacccg taacacctca aagccatcga
     5581 tatcaggcat catcacatct aaaagcacta agtctgggag ggtgctgcga agccgatcga
     5641 ttgcctggga tccacgaaag gcttcggtca cttgaaatcc atcatgctcc aaatttaagc
     5701 gtataaaacg taccatacgc tcttcgtcgt ctacaaccag tatgcgacgg tctcgaaagc
     5761 tattttccat agttctctac tcccgaacga agccgattat ttcttcatct tcctttccac
     5821 ttaagatctc gacaaaattc aatttatcca ttggccaaag
//
LOCUS       DBNGSX010000285         2797 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_92890, whole genome shotgun sequence.
ACCESSION   DBNGSX010000285 DBNGSX010000000
VERSION     DBNGSX010000285.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_92890"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /gene="plsX"
                     /locus_tag="AC16CB_08680"
     CDS             complement(<1..898)
                     /gene="plsX"
                     /locus_tag="AC16CB_08680"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIR3342583.1"
                     /translation="MRIILDAMGSDTHPEPELQAAVDAGRLFGDEIILVGHQDVLLPR
                     LKALAGDHPPVRVIHAPEVLEMDDKPAASARHKAENSMAIGMDLVKTNQADAFVTAGN
                     TGGAMANALFRLGRIRGVKRPALTALFPVRTGYCVVLDIGANADCKPEYLLQFAIMGS
                     VYAEKVMGVQKPRVALLSNGEEAGKGNELIKETYPLMKASGLNFIGNVEGKELFGGHA
                     DVVVMDGFTGNILLKSSEAVAKLITDMLRQELMSNVQTKVGGFLARPAFTRIKKMLDP
                     GEVGAVPLLGIDGLIFIGHGRSD"
     gene            983..1064
                     /locus_tag="AC16CB_08685"
     tRNA            983..1064
                     /locus_tag="AC16CB_08685"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1017..1019,aa:Leu,seq:gag)
     gene            complement(1157..2026)
                     /locus_tag="AC16CB_08690"
     CDS             complement(1157..2026)
                     /locus_tag="AC16CB_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR3342584.1"
                     /translation="MQNSLGQSRKPVLAPATSSLAGLPWWAIVMAATGLLLIYFILSD
                     DNYRQTFLFMQIGVVTTLRITLIAFPIATVIGLFVGLARTSKNTVIFTAATIYVEVVR
                     GIPLVVLILILAFGLVPIYVDLVNRLGTWGLTWANTQFIIGISTGMRDYSIRSIPMEL
                     RAILALSFGYGAFEAEVFRAGIQSIGRGQMEAARSLGMSYVQAMRLIILPQAIRRILP
                     PLGNDFIALLKDSSLATVLAVNELTQLTRIRRSSTFRVMEAFNVAAFLYLSMTILLSG
                     FVRLLERRMKITE"
     gene            complement(2111..>2797)
                     /locus_tag="AC16CB_08695"
     CDS             complement(2111..>2797)
                     /locus_tag="AC16CB_08695"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR3342585.1"
                     /translation="PDLGGQTIAVAVENAYPPFNTIDETTNEGVGWDYDTVREICARL
                     NCVPEFVQAAWDGIFPAMQAGEFDMLADGVTITAERDEIVDFSTPYVIVGQVLLVRVD
                     ETAGVDEFKADANRLVGTQLGTTNEMVAQDHFPTERIKSFEDFPAAVLALISKDVDAV
                     VIDNISALGFMEEYEGQLKTVGQLTSDEELGFVFPPGSELTAAVDAALQSMVDDGTLD
                     ELNKKWGLTQ"
BASE COUNT          686 a          736 c          731 g          644 t
ORIGIN      
        1 cgtctgacct tccgtgaccg ataaaaatta gcccgtcaat acctaataat ggcactgctc
       61 caacctctcc tggatccagc atcttcttga tacgagtaaa tgcagggcgg gctaaaaacc
      121 ctcccacttt ggtctgtaca ttactcatga gttcctgcct gagcatatca gtgattaatt
      181 tcgcgacggc ctcgctggat tttagcaaga tattacctgt gaatccatcc atcacaacta
      241 catcggcgtg gcctccgaag agttctttgc cctccacatt tccaataaaa ttcaaaccgc
      301 tggccttcat tagggggtaa gtttccttta ttaactcatt acctttacct gcctcttcac
      361 catttgagag gagtgctaca cgcggttttt ggacgcccat caccttttcg gcgtatacag
      421 agcccattat ggcaaattgg agcaggtatt ctggcttgca atcagcattt gcgccgatgt
      481 ctaaaaccac acaataaccg gtcctcactg ggaatagggc agtcagtgct gggcgcttga
      541 cgccgcgtat gcgtcccagg cggaaaagtg cattagccat tgcgccaccg gtattccccg
      601 cggtcacaaa tgcgtcagcc tggttggttt tcaccaagtc cattccaatc gccatcgagt
      661 tttcggcttt gtgacgggct gaggcagctg gcttatcatc catctccagc acctccgggg
      721 cgtgaatgac cctcacaggt gggtgatcgc cagccaatgc tttgaggcgt ggcagcaaca
      781 catcctggtg tcctacaagg atgatctcgt caccaaacag cctgccggca tcaacagccg
      841 cctgcaattc aggctcagga tgggtgtcgc ttcccatggc gtctaaaata attcgcatca
      901 agtctcctac gaatttgcgg gagttaaaat acaaggcgat gcttgacatc caccgcgtgg
      961 tgattataat accattgcca ttgcgctggt gctggaattg gcagacaggc atggttgagg
     1021 gccatgtgcc gcaaggcgtg tgggttcaac tcccacccag cgcacagatc ataaatcaat
     1081 tccggatgat tggggcagga agggttttcc tagatcatcc ggaatcgatt ttaatgcaga
     1141 gagatttttt ctaaactcat tcagtgatct tcatccgtcg ttccaataag cgcacaaatc
     1201 cggacaagag tattgtcata ctcaagtata ggaatgctgc gacgttaaat gcctccatca
     1261 cccggaatgt cgacgagcgc cggatacgtg tgagctgtgt aagctcgttg acggccaaga
     1321 ctgttgccag agatgaatct tttaataggg cgataaaatc attgcctaaa ggtggcagga
     1381 tgcgccgtat cgcttggggc aggatgatca ggcgcatcgc ctgtacgtag ctcataccca
     1441 gcgatcgggc tgcttccatt tgacctctac cgatagattg gataccagcc cggaagacct
     1501 cggcctcaaa ggcgccataa ccgaacgata atgcgagtat tgcacgcagt tccattggga
     1561 tggagcgaat tgagtaatct ctcattccag tggagatgcc aatgatgaat tgcgtattag
     1621 cccaggtcag tccccaagtt cccaaacggt tcaccaaatc gacgtagatg ggcactaaac
     1681 caaaagctaa gattaagatg aggacaacca aggggatgcc acgcaccact tcaacataga
     1741 tcgttgcagc tgtaaagata accgtgtttt tagacgtgcg tgccaagcca acgaacaaac
     1801 cgatcacagt agcgataggg aaggcgatca atgtgatgcg gagggttgtc accacaccaa
     1861 tctgcatgaa caggaaagtt tggcggtaat tatcatctga aagtatgaag tagatgagaa
     1921 gcagaccggt tgccgccatt acaatcgccc accaaggaag accggcaagc gaactggtcg
     1981 caggagcgag aacaggtttt cgtgattgcc caagcgagtt ttgcatcaca ccctccttca
     2041 catgccaaac gctgggatgg gggcacagcc ccatcccagt gtccacaaga aacagttact
     2101 agttatcccg ctattgagtg aggccccatt tcttattgag ttcatccagg gtgccgtcat
     2161 cgaccataga ctgtaaggca gcgtcgaccg cggcggtcag ctcggagccg ggtgggaaga
     2221 cgaagccgag ctcttcatcg gaggtgagct gcccaacggt cttgagctgg ccttcgtatt
     2281 cttccatgaa gcccaaggca gagatattat cgatcaccac ggcatctacg tccttggaga
     2341 tcagcgccag gacagctgcg gggaagtctt caaaggattt gatgcgctcg gtggggaagt
     2401 gatcctgagc taccatctcg ttggtggtgc cgagctgggt gcctaccagg cgattggcgt
     2461 cggctttgaa ttcatccaca ccggcagtct cgtcgacacg cacgagcaga acctggccaa
     2521 cgatgacata gggggtagag aagtcgacga tctcgtcgcg ctcggcggtg atggtgacgc
     2581 catcagcgag catatcgaac tcaccagcct gcatagcggg gaagatgcca tcccaggcgg
     2641 cttgtacgaa ctcgggaaca cagttgaggc gagcgcagat ctcgcgcacg gtgtcatagt
     2701 cccagcctac gccttcgttg gtggtctcat caatggtatt gaagggcggg taggcgttct
     2761 ccaccgctac tgcgatagtc tggcctccca ggtcagg
//
LOCUS       DBNGSX010000286        15394 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_369593, whole genome shotgun sequence.
ACCESSION   DBNGSX010000286 DBNGSX010000000
VERSION     DBNGSX010000286.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 15394)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15394)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15394
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_369593"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..735)
                     /locus_tag="AC16CB_08700"
     CDS             complement(100..735)
                     /locus_tag="AC16CB_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V4R domain-containing protein"
                     /protein_id="HIR3342586.1"
                     /translation="MDPIPKSGYYYANKFALITLKALEDVMGKNGVNAVLNLGHLPDL
                     IDNYPPDNLERGFDFADFTAINQALEELYGSRGGRGLALRVGRATFADALRNFGALAG
                     VGDLAFKVLPLQIKLRIGLPAMARIFSQITDQYSTVEERESDFIYTIHRCPQCWGRSD
                     LDKPVCFIGAGLLQESLKWVSGGNEFRVNESKCMAMGEKVCEYTIQKAPIG"
     gene            complement(747..1094)
                     /locus_tag="AC16CB_08705"
     CDS             complement(747..1094)
                     /locus_tag="AC16CB_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HIR3342587.1"
                     /translation="MVERLRDLQASSPDIEASAVVSVDGLSIASALPQGVEEDRVSAM
                     SAAMLSLGERIASELGRGTLDQVYIKGEKGYVILMSVGEEAVLTALAREQAKLGLIFL
                     DMRRATEDLAKLI"
     gene            1269..2747
                     /locus_tag="AC16CB_08710"
     CDS             1269..2747
                     /locus_tag="AC16CB_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HIR3342588.1"
                     /translation="MPFKNLREFIKLLEHRGQLTCIKVPVSPILEVTEITDRVSKGSP
                     DYNKALLFENPSGFDMPVLINAFGSAERMAWALGVDQLEDLNHNLAKIVDPRLPRGFN
                     EMVSRGRDLWSVFKSIGPGPKKVRRAPVQEVVHIEDASLDKLPILQCWPKDGGRFITL
                     PQVITRDPQTGARNVGMYRLQEVDSRTLMMHWQRHKGGAEHERKALQAGYTPAQHIPA
                     AIVLGGDPASMWCASAPLPPDMDEYLLAGYLQGAPVPFVDCITQPLEVPAQAEIVIEG
                     YVDPNEHLPEGPFGDHTGYYTPVEPFPVFHVTAITHRKDPIYPATIVGIPPMEDLWMG
                     KATERLFLPLMRLFLPEIVDVNMPAEGVFHNLVLVSIKKRYPGHARKVIFGLWGLALM
                     SLSKAIVVVDEWVDVQNLSQVSWQVLGNVDWSHNIIIAEGPVDHLDHASYQHSFGGKI
                     GIDATAKQLEEGYSRDWPEVVQMSPEVKARVDEIWSKLKLDR"
     gene            2835..3794
                     /locus_tag="AC16CB_08715"
     CDS             2835..3794
                     /locus_tag="AC16CB_08715"
                     /inference="COORDINATES: protein motif:HMM:NF024772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3342589.1"
                     /translation="MRYFSTKLVIFVILLSMLLSACAPAAAPAEPVTLKIAVLRILDT
                     LPMYVAQQEGLFDAKGITVEFIPAASAAERDQIITAGQADGMINEVVSTLLYNKDQIQ
                     IQIVRFARTATPTSSQYSVLAAQQSGITDVQGLKGVEIGISQGTVIEYITDRLLEAEG
                     LAPEDIKIVAVPKIDVRMNLLGTGELKAATLPDPFSSLALQQGAVLVTDDTRHPEYGY
                     STISFRKPVIDEHPEAIKAFLAAIEEATALINADPTKYENVLTEQKLIPAPLLGSYKF
                     PPFPPASVPSQDQWDDVLTWMKSKGLMDKDVSYTDSVNPYFLP"
     gene            3857..4621
                     /locus_tag="AC16CB_08720"
     CDS             3857..4621
                     /locus_tag="AC16CB_08720"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3342590.1"
                     /translation="MIELRSVTYAYQQGPPIFQDYDWKAERGEAWAVLGPSGCGKTTL
                     LYLLVGLRFPTSGQVMIDGQPLVRPRPHTGLILQDYGLLPWATVRQNAELGLQVRSFY
                     GPDGKHAPDDFSAEVDVAPWLERLGLSSVTNKYPAQISGGQRQRTAIARTLALKPDLL
                     LMDEPFSSLDAPTRESLQNLTLELQFEQNLTLVIVTHAIEEAAIMGRKILLLGTPPNR
                     EAQVVENPGATQSNFRETAQYLEMCKHLRSLMETPT"
     gene            4638..5378
                     /locus_tag="AC16CB_08725"
     CDS             4638..5378
                     /locus_tag="AC16CB_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3342591.1"
                     /translation="MKKRDLLFAVAALIFLWQLVAMAVNRPILPTPWVVTQTFVDELR
                     SDLLVHFRASLWRVIASTLLAIALAAPAGLVLGQSKKLNSFFSPLIYLVYPVPKVVFV
                     PIVLLFLGLGDSPKIVIIFLILFFQVLVLVRDQAAALRPELIQSVRSLGAGRRALFRF
                     VYLPASLPAILTALRQSVGTAVAVLYVAELYATQKGLGYYIYLNGSTLLNYPAMYAGI
                     VAMSLLGLGLYFSVDWLEKRLCPWLFTS"
     gene            5430..5987
                     /locus_tag="AC16CB_08730"
     CDS             5430..5987
                     /locus_tag="AC16CB_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR3342592.1"
                     /translation="MRIHQSSFLRHTFAILIILAMVLGAFPNAAQAATCDTYYTVKSG
                     DTKTKIAKLYKIKWREIAEANDLEEPYNLKVGQKLCIPYNADDDEELANPKLRLRVSA
                     THTIVTLTLSGLSDKKAVFIVRMRDATIGIGGWQKLGRIKLKKNSTSKETFRIPKEFK
                     NTLYLRVCIKNSSTDELTCQVVLHP"
     gene            6087..6947
                     /locus_tag="AC16CB_08735"
     CDS             6087..6947
                     /locus_tag="AC16CB_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013615246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     polyprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HIR3342593.1"
                     /translation="MKSVRDFLELIKFEHTVFALPFAYLGMLLAARGWPGWKEFIWIT
                     IAMAAARTVAMGFNRIADRWIDAHNPRTAKRPLVTGMISTRVAWGGTFVAAAVFTLAA
                     WQLGPLPFKLLPGAFLFLFGYSFTKRFTWLSHYIVGFTDGLAPMGAWAAVRGSLFTQA
                     DLPAWIMLGVVTLWIGGFDLIYACQDVPYDQQDGLHSVPARFGIPTALRLSTVSHILT
                     VGLLAILGVLMGLGWPYWIGLLLVAGLLTWEHTLVHPDDLSRVDMAFFNLNSYISVTL
                     FLSILGALYV"
     gene            6940..8145
                     /locus_tag="AC16CB_08740"
     CDS             6940..8145
                     /locus_tag="AC16CB_08740"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIR3342594.1"
                     /translation="MYSILGLFGKIGARRSHRWALGLILLAALISYRPALAAGDDPGV
                     LCLPDVYLQVASDCTPLGPSAYLTHLAEKNFTFTAAPLTYKRPDFSLTNVNTRYGQVN
                     KTNAPVYGSLEDALKGNKKEATRQIDAVFSYISYTEDVVVDGKRFYMIDQDAWMTAND
                     IVRLGAVPLFQGLTFNHTPNHSFGWVLSYLSQGPVETKRTPGWQNQDYTGHTLNNHEI
                     VPVYSTEVVNEEEWYMVGPDEWLHQNVVARVVPNPTPPQGVTGDRWIEINLFEQTLAI
                     YEQRQLVFATLIASGLDPFWTRPGLFQIYDKLDTTPMRGAFERDRSDAYYLEDVPWTM
                     YFDQARALHGAYWRANLGFPQSHGCVNLSVGDSRWIFNWANIGDWVYVWDPSGKTPID
                     PSVYTAGGA"
     gene            8232..9629
                     /locus_tag="AC16CB_08745"
                     /pseudo
     CDS             8232..9629
                     /locus_tag="AC16CB_08745"
                     /inference="COORDINATES: protein motif:HMM:NF015531.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
     gene            9668..11737
                     /locus_tag="AC16CB_08750"
     CDS             9668..11737
                     /locus_tag="AC16CB_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HIR3342595.1"
                     /translation="MLTLAEKILFVIAILASLIAAYRVADRIIRTIRRGQGEVDWLIA
                     QKRLIGVLVKTIALQPVFRLRFWPSLFHGLVAWGFIYYLLVNLFDVLAGYIEGFAIPG
                     VLGDLYRLGADLLSVGALVGMLALIIRRFGMKPATLSARQDVLLHPKARAGIRRDSAI
                     VGGFILVHVGSRFFGQSVYIAATGHDAWQPFASLVAKLWAGWSDAALTFGEHAAFWLA
                     LGTILAFIPYFLYSKHIHLFFAPINFLLKPERLSIGELGRLDFDDESIEQFGATRLED
                     LGWEQIMDAYACIMCYRCQEVCPAYNTGKVLSPAALEINKRYFLNYEGERMAKGEPSE
                     KTLLEFAIPAEAVWACTACGACIDICPVGNEPMRDILDIRRALVLMENDFPEQLQTAF
                     RGMERTMNPWNVPPPERMKWAEGLEVSTIDALPEPDVLWWVGCAPATDARAQKTARAF
                     AEVLNAADVSYAVLGEMEQCTGDSARRAGNEFLFNEMALANVEMLNEVKPKRIVTTCP
                     HCLHTLKNEYPAFGGHYQVIHHTQLINELVGAGKLKLKESVGSSQSVGDMQSSTSGSQ
                     KSTIAFHDPCYLGRQNDIIEEPRLVLEQANANLAELANTGKKSFCCGAGGAQMWKEEE
                     HGAERVNQRRFKEAQESGVDVLAVGCPFCMVMLNDARKDADSQIEVLDVAEIVAMAID
                     TGDLPQS"
     gene            complement(11734..12186)
                     /locus_tag="AC16CB_08755"
     CDS             complement(11734..12186)
                     /locus_tag="AC16CB_08755"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012579.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR3342596.1"
                     /translation="MGWITVESIAVGVSVDTWQDAVRAAGHLLVRVLAAESRYIDAML
                     RTAEELGPYVVLAPGLALPHARPEDGALTVGFSAVTLAAPVEFGNPDNDPVRLVIAFC
                     APDSTSHLQSLSQLARVLEKPGFIEQAASAQDEVELATILNGESLSQG"
     gene            complement(12196..12945)
                     /locus_tag="AC16CB_08760"
     CDS             complement(12196..12945)
                     /locus_tag="AC16CB_08760"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HIR3342597.1"
                     /translation="MAEVIFAERYFQTALSILQQVVRDESKGIDAAAELIVESLAAGG
                     VLHVFGSGHAHLFAEELAYRAGGLAPVNPIVDYGYTLMGGSASRSTRLERLEGYAIAQ
                     LQSYDLRPGEVLLAVSQSGINPGPVEAALFAKEKGLKVVAVTSLSHSGNLPSRHSSGK
                     RLFEIADVVIDSHVPMGDALVELSPGSPKVSPGSTVVGAAILQALVAEVSARLLQRGA
                     EPPVWISSNIPGGDEHNLRLAEKYPSRLKAF"
     gene            complement(13072..14370)
                     /locus_tag="AC16CB_08765"
     CDS             complement(13072..14370)
                     /locus_tag="AC16CB_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HIR3342598.1"
                     /translation="MDIIINIAKFIADNIFSQPSLLVGLIAMVGLIAQRKAFSDVVTG
                     TLKTAVGFLIMAKGAEIVVDALLKFTPILQAAFNMEKSLFDAFGGAGLGTLMGNHGST
                     AALIMTFGFLFHVVLARITRLKYIYLTGHLMFWISLVLVAVMLEINPNTSVLAMVVVG
                     SIIAGVYWTIQPAWIQPLMRKVTGTDELAYGHTSATNVWLAGTLGKYVGKADDSTEKL
                     QLPKSLTFFRDVTVATAVVIAIVTVIAALVAGPTKVDSGTLNYVVFAALQGLTFAVGI
                     AVLLTGVRMVLGEIVPAFRGIALKIVPGARPALDCPITFDYAPTAVLVGFISAFVVFM
                     VCMIVFGAIGWAVIVPPMIMLFFPGGAGGVFGNATGGVRGAILGGVILGLFLAFGQAI
                     TAPMLSTTSPELAQLADPDWYLIVWIFKPVLSLILPLFGG"
     gene            complement(14538..14819)
                     /locus_tag="AC16CB_08770"
     CDS             complement(14538..14819)
                     /locus_tag="AC16CB_08770"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014368.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIR3342599.1"
                     /translation="MSLKIITVCGVGMGSSLLLRMYTEDVLKELGLEANVEASDATQA
                     RGARADLILTSPALVEVCSGGNAPVKAIKSFVNKAEIRAALEEFLSERG"
     gene            complement(14863..>15394)
                     /locus_tag="AC16CB_08775"
     CDS             complement(14863..>15394)
                     /locus_tag="AC16CB_08775"
                     /inference="COORDINATES: protein motif:HMM:NF019322.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3342600.1"
                     /translation="GKGTFVGEVRFEQDRTLTGFSEEMQSLGMTTSSRVMDTAVLPAT
                     AELAGILQVHPNSGVICLARLRLANGHLIAYEKAHLPQSLCPDLLEHDFSNESLYSVL
                     RQDYGLKLVTAEQTVEAALANRQELRMLQLKSPAAVLRMKRVTHTDQGKVAEYTESVY
                     RGDRYQLSTTLHITQD"
BASE COUNT         3528 a         4207 c         4120 g         3539 t
ORIGIN      
        1 actaaataaa gcggagacca atttcggcag gttctccgct tcgttatatg taggtataac
       61 gagatggact tgcacagtaa ttatctcacc tttgatagat tacccaatcg gtgccttctg
      121 aatggtgtac tcgcagactt tttcgcccat ggccatgcac ttggattcgt tcacacgaaa
      181 ttcattaccc ccagaaaccc acttcaaact ctcttgtaac aggccagcgc caataaagca
      241 gaccggttta tctagatcag atcttcccca acactgcggg cagcggtgaa tggtataaat
      301 gaagtcgctc tctcgctctt ccactgtgga gtattgatcc gtaatctggc tgaatatgcg
      361 ggccatggct ggcaatccga tgcgcagctt aatttgaagc ggcaagacct taaaagccag
      421 atcacctacc ccagccaggg cgccgaaatt tcgcaaggca tcggcaaatg tagcccggcc
      481 gacgcgcagg gccaagcccc gcccgcctcg cgaaccgtac agctcctcca gagcttgatt
      541 aatcgcagtg aagtcggcaa aatcaaaccc gcgctccagg ttatcaggcg ggtagttatc
      601 aatcaaatcg ggtaagtgac cgagattcaa cacagcgttg acgccgttct tacccataac
      661 atcttctaaa gccttaaggg tgatcagggc gaatttattg gcgtagtagt agccactctt
      721 tgggatggga tccatagtgc cgccgattat atcagtttgg caaggtcttc cgtggcgcgg
      781 cgcatgtcca agaatataag cccaagcttg gcttgctcgc gcgccagagc ggtgagcacc
      841 gcttcttcgc caacggacat taagattacg tagccttttt cccccttgat gtatacctga
      901 tccaacgtgc cgcgccccaa ttctgaggcg atacgctctc cgagggatag catcgcggcc
      961 gacatcgctg aaacccggtc ttcctccacc ccttggggta aagctgaggc aatgctcaga
     1021 ccatcaacgc tgaccactgc tgaagcctcg atgtctggcg aagaagcctg cagatcgcgc
     1081 aggcgttcca ccatcaattg ggagcgtgat ttggtcaata taaatactcc tatagggaca
     1141 ctgaaagcca gctaagcaaa ttgaattacg aatttgcgaa agtaaacaaa gctaatttag
     1201 cacacgggtg gctgcgtgtc aagccaatca tgctataata agccggtttc ccaaccggag
     1261 tgtaaggtat gccattcaaa aacttgcgcg agtttatcaa gctattagaa catcgcggcc
     1321 aactcacctg cattaaagtt cctgtatctc ccatattgga agtaaccgaa attactgacc
     1381 gtgtctctaa aggttcgccc gattataaca aggcactgct ctttgaaaat ccttccgggt
     1441 tcgacatgcc cgtgctgatc aatgcctttg gatcggcaga gcgcatggca tgggcactgg
     1501 gagtggatca actcgaagat ctgaatcata acttggcaaa gatcgttgat ccgcgcctgc
     1561 cgcgcgggtt caatgagatg gttagccggg gacgcgacct gtggagtgta ttcaaatcta
     1621 ttggcccggg gcctaaaaag gtgcgccgcg caccggtcca agaggtggtt cacattgagg
     1681 acgcctcgct ggacaaactg ccgattttac aatgctggcc caaggacgga ggtcgcttca
     1741 taacccttcc acaagtgatc acccgcgacc cacagacagg tgctcgcaac gtgggcatgt
     1801 accggctgca ggaggtcgat tctcgcactt taatgatgca ctggcagcgt cataaaggcg
     1861 gagcagaaca tgaacgcaaa gccctccagg cagggtacac acctgcgcag catatcccgg
     1921 cagccatcgt gctgggagga gacccggcct ccatgtggtg tgcctcagcc cccttgcctc
     1981 ccgatatgga cgaatacctg ctggcaggat atttgcaggg cgcaccggtt ccctttgtgg
     2041 actgtataac tcaaccgctc gaggtgcctg cccaggccga aattgttatc gaaggctacg
     2101 tggacccaaa cgaacacctt ccagagggac ccttcggtga tcacaccggt tactacaccc
     2161 cggtggagcc attcccggta ttccacgtaa ccgctatcac ccatcgcaag gacccaattt
     2221 acccggctac gattgtgggc atcccaccga tggaggattt gtggatgggc aaggccacag
     2281 agcgcctttt cctgccgctg atgcgcctgt ttttgccaga gatcgtggat gtcaatatgc
     2341 ccgccgaagg agtttttcac aacctggtgc tggtatcgat caagaaacgc tacccaggtc
     2401 acgcccgcaa agtgatcttt ggcctttggg gtctagcgct gatgtctctc tcgaaagcca
     2461 tagtcgtggt agatgagtgg gtggatgtac agaacctctc acaagtttcc tggcaggtct
     2521 tgggtaacgt ggattggtca cataatataa tcatcgccga aggtcctgtg gatcacttag
     2581 accacgcgtc ttaccagcat tcgttcggcg gcaaaattgg catcgatgcc accgccaagc
     2641 aacttgaaga aggctattcg cgcgactggc cagaagttgt gcaaatgtcg ccggaagtta
     2701 aggcgcgcgt cgatgagatt tggagtaaac tcaaattgga ccggtaacta ccgttcaata
     2761 cgaagatggt ggatttcttc tcgcataccc attttctggt tacacaccta tcaatcaaag
     2821 gagaaatttg atcgatgaga tatttcagta ccaagctcgt catttttgtc atcctgctca
     2881 gcatgctatt aagcgcctgt gcaccggctg ctgcccctgc tgagccagtg actctgaaaa
     2941 ttgcggtatt acgtatcctg gatacactgc ccatgtatgt ggcgcagcaa gaaggcttgt
     3001 ttgatgcaaa aggtataacg gtagaattta tcccagctgc atccgctgcc gagcgcgacc
     3061 agatcatcac cgccggtcag gcagatggca tgatcaacga ggtcgtctcg actcttctct
     3121 acaataaaga ccagattcaa atccagatag tgcgctttgc ccgcacagct actcccacct
     3181 catcccagta ctccgtcctg gccgcacagc agagcggcat caccgatgtg caaggcttaa
     3241 agggtgtaga gatcggcata tctcagggaa ctgtcatcga atacattacc gatcgcttgc
     3301 tggaggcaga aggcctggcg cctgaagaca tcaaaatcgt cgctgttcca aaaatcgatg
     3361 tgcgcatgaa cctgctcgga accggcgagc tgaaagctgc cacgctcccc gatcccttct
     3421 catccctcgc tttgcagcag ggcgctgtgc tggtcacaga tgacacgagg cacccagaat
     3481 atggctacag caccatctcc ttccgcaagc cggtcatcga tgagcacccg gaggcgatca
     3541 aggctttcct ggcagccatc gaagaagcca ctgcgctgat taacgccgat ccgacaaagt
     3601 atgaaaacgt gctaaccgag caaaagctta tccccgcacc cctgctcggt agttacaaat
     3661 tccctccttt cccaccagcc tcggttccat cgcaggatca gtgggatgat gtgttgacct
     3721 ggatgaaaag caagggttta atggataagg atgtttccta taccgattct gtaaacccat
     3781 attttctgcc ttaaaagatg ccggatgaga gtcatgatca tgaccctcat ccggctgctt
     3841 accttataaa tgaagcatga tcgagctgag gtctgtaacc tacgcctatc agcaaggccc
     3901 ccctatcttc caggactatg actggaaggc cgagcggggc gaggcgtggg cagtgcttgg
     3961 tccttccggt tgcggtaaga ccaccttgct ctatttgttg gtcgggctgc gattcccgac
     4021 ctctggtcag gtgatgatcg atgggcaacc gctggtgcga cccaggccgc acactggtct
     4081 gatcctgcaa gattacgggc tgctgccctg ggcaaccgtc aggcaaaatg ccgagctggg
     4141 attgcaggtg cgcagctttt atggacccga cgggaagcat gccccggacg atttctcagc
     4201 agaagttgac gttgcgccct ggttggagag actgggtctc agctctgtga ccaataagta
     4261 ccccgctcaa atctcaggtg gtcagcgcca gcgcacagcc atcgctcgca ccctggcgct
     4321 caagccagac ctgctcctga tggacgagcc tttctcctca ctggatgccc ccacacgcga
     4381 aagtttgcaa aatctgaccc ttgaattgca attcgagcaa aatttgaccc tggtgatcgt
     4441 cacccacgct atcgaagaag cagccatcat ggggcgcaaa atcctgctcc tcggcacccc
     4501 tcccaatcga gaagctcaag tggtggaaaa ccctggtgca acccaatcaa actttcgaga
     4561 gactgcacaa tacctggaaa tgtgcaagca cttgcgctct cttatggaga caccaacctg
     4621 agagaggtaa acctgtaatg aagaaaagag accttctctt tgctgtcgct gcactaatct
     4681 ttctctggca gctggttgcc atggcagtga accggccaat cttgcccacg ccgtgggtcg
     4741 tgacgcaaac cttcgttgat gagctgcgca gcgacctgct ggtccatttc cgggcgagcc
     4801 tttggcgggt gattgccagt acgctgctcg caattgcgct ggcggctcct gcaggactgg
     4861 tcttaggcca gtcaaagaag ctgaacagct ttttctctcc gctcatttac ctggtctacc
     4921 ctgtgccgaa ggtagtcttc gtgcctattg tcctcctttt cctcggcctg ggcgattctc
     4981 ccaagatcgt gattattttt ttaattttat ttttccaggt attggtgttg gtacgtgacc
     5041 aggcggctgc cctgagacca gaattaattc agagcgtgcg cagcctggga gcagggcggc
     5101 gggctttatt tcgctttgtc tatctgccgg cgagccttcc ggcgatattg acggctttac
     5161 gacagtcggt aggcacagca gtggctgtgc tttatgtggc tgagttgtac gccacccaaa
     5221 aaggcctggg ttattatatt tatctgaatg gcagcacgct gttaaactac cccgctatgt
     5281 acgcagggat tgtagcgatg agcctgctgg gattgggact gtatttcagc gtggactggc
     5341 ttgagaagcg cctctgcccg tggctgttca caagctgatt tagtgtataa tctccacagg
     5401 aggtccggga tcaaagaaag gagtaataaa tgaggatcca tcaatcatcg ttccttaggc
     5461 atacgttcgc gatcctgatc atactcgcta tggtgttggg ggcgttcccg aatgcagccc
     5521 aagctgccac ttgcgacacg tactatactg tcaagtcagg tgatacgaaa accaagatcg
     5581 cgaaattgta taagatcaaa tggcgggaga tcgctgaggc gaacgatctg gaagaaccat
     5641 ataacctcaa agtagggcag aagctctgca tcccgtacaa tgctgatgac gacgaagaac
     5701 tagccaaccc caaactaaga ttgagggtca gtgccactca tacaatagtg acacttacgc
     5761 tatcgggttt gtcggataaa aaagccgttt ttattgtgcg catgcgtgat gccacgatcg
     5821 gcattggcgg atggcaaaaa ctgggcagga taaagctcaa gaagaactcg acctccaaag
     5881 agaccttccg tatccccaaa gagttcaaga acacgcttta cctgcgtgtc tgtattaaaa
     5941 actcctccac cgatgagcta acctgccagg tagtcttgca tccgtaatat ttgactccaa
     6001 cggaaaggaa cccgaagaat tgggtgaaca ggcagctaca acgccgcgat cacccaattt
     6061 tccgttctac gctataatag catcccatga aatctgtacg agatttcctc gagctcatca
     6121 aattcgagca cactgtcttt gcgctgccgt tcgcctacct gggtatgctg ctggctgcca
     6181 gaggctggcc cgggtggaag gaatttattt ggatcacgat tgcgatggct gcagcgcgca
     6241 cggtggcgat gggtttcaac cgcatcgccg accgctggat cgacgcccac aacccgcgca
     6301 cagccaaacg ccccctggtc accggcatga tatctacacg cgttgcctgg ggtgggactt
     6361 ttgtcgcagc ggctgttttc accctggcag cctggcaact aggcccgcta ccgttcaagt
     6421 tattgcccgg cgcgtttcta ttcctgttcg ggtactcatt caccaagcgc ttcacatggc
     6481 tttcccacta cattgttggt ttcacggacg gcttagctcc aatgggagcc tgggcggcgg
     6541 tgcgcggttc gctattcacc caggccgatc tacccgcctg gattatgctc ggtgtggtca
     6601 cgctgtggat tggcggcttc gatctcatct atgcctgcca ggacgtgccc tatgaccagc
     6661 aagatggcct gcattcagtc cctgcccgtt ttggcatccc caccgcctta cgcctgtcta
     6721 cagtcagtca tattctcacc gttgggctct tggcaatcct gggagttttg atgggactcg
     6781 gctggccgta ttggattggt ctgctcttgg tggctggact gctgacctgg gaacacaccc
     6841 tggtccatcc ggatgacctc agccgggtgg atatggcttt cttcaatctc aatagttata
     6901 ttagtgttac gctttttctt tcaatattag gtgctttata tgtatagtat tctcggttta
     6961 ttcggaaaaa ttggcgcgcg gcgatctcat cgttgggcgc tgggcttgat cttgcttgct
     7021 gcccttattt catatcgacc tgccctggcg gcaggtgatg atcctggtgt gctctgcctg
     7081 ccggacgtct acctgcaagt cgccagcgat tgcacccctc ttggtccctc ggcgtatctg
     7141 actcacctag ccgagaaaaa cttcaccttt actgctgccc cgctcacata taaacgtccg
     7201 gatttttcct tgaccaacgt caatacgcgt tatggtcaag tcaataaaac aaacgcgccg
     7261 gtatatggtt ccctagagga tgctctgaag ggcaataaaa aagaagctac caggcagatt
     7321 gatgccgtat ttagttatat ctcctatact gaagatgtgg tagtggatgg caagcgcttt
     7381 tatatgatcg accaagatgc ctggatgaca gccaatgata ttgtccgcct tggtgcggtg
     7441 cccctattcc aagggcttac attcaaccac acgcctaacc attcatttgg ttgggttcta
     7501 tcctacctca gccagggacc ggttgagacc aagcgcacac ctggctggca gaatcaggat
     7561 tacactggcc atacactcaa caatcatgag atcgtcccgg tgtactccac agaggtcgtc
     7621 aatgaggaag agtggtacat ggttgggccg gatgaatggc tgcaccagaa cgtggtggct
     7681 cgtgttgtgc ctaaccctac tccgccccaa ggtgtgactg gtgaccgctg gatcgaaatc
     7741 aacctgtttg agcaaaccct ggccatatat gagcaacgcc aattggtctt cgctacactg
     7801 atcgccagcg gcctggaccc cttctggacg cgcccgggcc tgtttcaaat ctacgataaa
     7861 ctggatacaa cacccatgcg cggggccttt gagcgcgacc gctctgacgc atattatctc
     7921 gaagatgtcc cttggacgat gtattttgac caagcgcgcg ccctgcacgg tgcatattgg
     7981 cgcgccaacc tgggtttccc tcaatcacac ggctgtgtca acctatcagt aggtgattct
     8041 cgctggatct tcaattgggc aaacatcggc gattgggtgt acgtttggga cccttcgggt
     8101 aagacaccaa ttgacccatc ggtttatact gctggtggcg cataaggctg catcccggct
     8161 tttattgaca attgtctgct tcctggctgc aacgtaagca ccatttctct ggttcgtgac
     8221 cgctgctgca catgaaacca cgctcgcatg ctgtttggat tctccctttt gtgctagttt
     8281 tgctggcttg ccaggctgtc accggtttgg tgcagcccac cgcgaccacc tcgctattgc
     8341 ataccagcaa aaacctgccc aagccggtct ctcccacacc aacctctact gagacacccc
     8401 ttccgactag aaccgctacc cccaccgaaa cacctacacc aacccttaca gctactgcaa
     8461 ccattgtccc aagcccctcg gctctccaac tcaaggtttt tgaggagctc tggcacgccg
     8521 ttctgaccga ttacctgtac cctgattaca atggccagga ttgggaggca gtgggagaaa
     8581 aataccgtca acgcattaag gctggcatga gcgcccatga tttttacaat gcgatggagg
     8641 agatgattgc cagcctgaac gatgagcact cggttttctt gcgccctgag gaggcaattg
     8701 aggaagatgc tgaattttct ggtgccaacg actatgtggg catcggggtg cttaccaatg
     8761 tggttccaga gcgcaagcgg gctgtcattt tgatcgtgtt tcccaacagc cccgcagaag
     8821 aagccggcct gaaatcccac gacagcattt tggctgtgga tggtgagccg cttattgacg
     8881 agaccgggat gcggcgaaac ctgctgcgcg gtcctgaaaa cacaagcctc gaattgactg
     8941 tacaaacccc aggggaagct ccgcgccagg tcagccttac ccggcggcgc gtgaccggct
     9001 cagtgccggt gccttatgcc gtatttacca ctccacaagg caaacgcgtt ggctatattc
     9061 tgctcgttac cttttcggat gagacgatag ataaccaggt cgagaaagcg ctgaaagcgc
     9121 tgactgccca aggccgtctg gatggcttga tcctggataa ccgtgttaac gctggtggcg
     9181 cagacaatgt cgcccgagcc gttttgggtt tctttacgaa agggacaata ggataattct
     9241 atcgccgaga ccaatccagg cgcgccttta acgtgattgg aactgaaatc aatggttcgt
     9301 atgaagtgcc cctggtcgtg ctggtaggac cagacacggt gagcttcgga gagatattct
     9361 ctggggtgtt gaaggacaat ggacgagcct atgtcattgg ggagctgaca cagggtaatg
     9421 ttgagctact ttggggttat aacttcgaag acggctcacg cgcctggctt gcccaagaga
     9481 aattccgccc tcgaaacaac cccgatcagg attgggaaga gaccggcatt atccccgacc
     9541 tgactgtagc cactaattgg gatgaaatcg tttcagacac tgaccctgca attctggcag
     9601 cattggagta tatggacgga ttagagtaaa atacgcctac tcccgacacc gagaaagcgg
     9661 aggcatcatg ctcacactcg ccgaaaaaat cctgtttgtc atcgccatac tggcctcgct
     9721 catcgcagca tatcgagttg cagaccgcat cattcgcacc atccgacgcg ggcagggaga
     9781 agtggattgg ctcatagctc aaaaacgcct gatcggcgtg ctggtcaaaa ccatcgcctt
     9841 gcagccggtc tttcgcctcc gtttttggcc cagcttattc catggcttgg tagcttgggg
     9901 atttatctat tatctgctgg tcaatttgtt cgatgttctg gctggttata tcgagggctt
     9961 cgccatccca ggtgtgcttg gagacctgta ccgcttgggg gcggacctac tcagcgtagg
    10021 ggccctggtt ggtatgcttg ccctgatcat ccgccgattc ggcatgaagc ccgcgacctt
    10081 aagcgcccgc caggatgtgc tgctgcaccc taaagcgcgc gctggcatcc gacgggattc
    10141 ggcaatcgtc ggtggattca ttttggtgca tgtggggtcg cgcttcttcg gccaatccgt
    10201 gtatatcgct gccacgggac atgatgcctg gcaacccttt gccagccttg tagccaagct
    10261 gtgggctggg tggagcgatg cggctttaac cttcggcgaa cacgccgctt tctggcttgc
    10321 tttgggcaca atcctggcat ttattcccta tttcctgtac tccaagcaca tccacttatt
    10381 ttttgccccc atcaacttcc tgcttaagcc tgaacgcctc tccattgggg agcttggtcg
    10441 tttggacttt gacgacgaaa gcatcgagca atttggagcc acccgcctgg aagacctggg
    10501 ttgggagcag attatggatg cctatgcctg cataatgtgc taccgctgcc aggaggtctg
    10561 cccggcctac aacacaggca aggtgctctc cccagctgcg ctggagatca acaagcgcta
    10621 ctttctgaac tacgaaggcg aacggatggc taaaggcgag ccgagcgaga agactctgct
    10681 ggagtttgct atcccagccg aagcagtttg ggcatgcacg gcttgcggag cctgcataga
    10741 catttgtccg gtgggtaacg agcccatgcg cgatattctg gatatccgcc gggcactggt
    10801 tttgatggaa aacgactttc cggagcagct gcagactgcc tttcgtggta tggagcgcac
    10861 catgaatccg tggaatgttc cgcctccaga gcgcatgaaa tgggccgagg ggctggaagt
    10921 ttccaccatt gacgcgctac ccgagcccga tgttttgtgg tgggtaggtt gtgcaccggc
    10981 gactgacgcg cgcgcccaga agacagctcg cgcctttgct gaggtattaa atgctgcgga
    11041 cgtgagctac gccgtgctag gtgaaatgga gcagtgcacc ggcgattcag ctcggcgggc
    11101 tggtaacgag tttcttttca atgagatggc actggcaaat gtggaaatgc tcaatgaggt
    11161 taaaccgaag cgcatcgtca ccacctgccc gcattgcttg cacaccctga agaatgagta
    11221 tccggcattt gggggccatt accaggtgat acaccatacc cagttgatca atgagctggt
    11281 gggggcgggg aagctaaagc tgaaggaatc tgtcggtagc agccaatcgg tgggcgatat
    11341 gcaaagctcc actagtggca gtcagaaatc cacaatcgcc tttcacgatc cctgttatct
    11401 aggtcgccaa aacgatatca tcgaggagcc acgcttagtg ctggaacaag caaacgccaa
    11461 tttggctgag ctggcaaaca ctggcaaaaa gtccttctgc tgcggtgcgg ggggcgcgca
    11521 gatgtggaag gaggaggaac acggggccga gcgcgtcaat cagcgccggt tcaaagaagc
    11581 tcaagaaagc ggcgtagatg tgctcgccgt gggctgcccc ttctgcatgg tcatgctcaa
    11641 cgatgcccgt aaagacgctg acagccagat cgaagtgctg gacgtggccg agatcgtagc
    11701 gatggcgatc gacacggggg atttacccca gtcttagcct tgagacaggc tctcgccatt
    11761 caagattgtt gctaattcaa cctcatcttg tgccgaagca gcctgctcga tgaagccggg
    11821 tttctccagc acgcgcgcaa gctgagatag tgattgcagg tgggaggttg aatcgggggc
    11881 acagaaagcg atcaccaggc gcactgggtc gttatcgggg ttgccgaact ccacaggagc
    11941 agccagagtg actgcagaga agcccacagt gagggcaccg tcttccgggc gggcgtgtgg
    12001 tagcgccaaa ccgggggcta gtaccacgta agggcccaat tcttccgcag tgcgcagcat
    12061 ggcatcgata tagcgcgact cggcagccag cacccttacc agcaaatggc cggctgcacg
    12121 cacggcatct tgccatgtat ctacagacac acccactgca atcgattcta cagtaatcca
    12181 gcccatgacg atctcttaaa acgccttcag ccgagaaggg tacttctccg ccagccgcag
    12241 gttgtgctcg tccccgccgg ggatgttgga gctgatccag accgggggct cggcgccgcg
    12301 ctgcagcagc cgggcggata cctcagccac caatgcctga aggatagcgg ctccaaccac
    12361 tgtggacccc ggcgagactt tcgggctgcc agggcttagt tccacaaggg catctcccat
    12421 agggacatga ctgtcgatga ccacatcggc gatctcaaag aggcgcttgc cgcttgaatg
    12481 tcggctgggt aggttacctg agtgagatag ggaggtaact gccaccactt tcagaccttt
    12541 ctctttggcg aacaaagcag cttcaaccgg acctggattg attcccgatt gggagaccgc
    12601 cagcagcact tctcctgggc gaagatcata gctctgcagt tgggcaattg catacccttc
    12661 gaggcgttcc agccgcgtac tgcgagaagc gctgccgccc atcagcgtgt atccataatc
    12721 cacgatgggg ttaaccgggg ccagtccacc agcccggtaa gccaattcct cggcaaaaag
    12781 gtgagcgtgt cccgatccaa aaacgtgcaa tactccaccg gctgcaaggc tttcaacgat
    12841 caactccgcg gccgcatcga tccctttgga ttcatcgcgc acaacctgct ggaggatgga
    12901 gagggcagtt tgaaaatatc gctcagcaaa tattacttct gccattctct tttgttttcc
    12961 ttaaaagagg tactttgggg gtctgagggt tctacgagcc cgcaaacccc ctatccaatt
    13021 tttctcccct ccctgatatt gagggagggg tatcaatgcg agtattcccg atcagccccc
    13081 aaagagaggc aatatcaggg acagaacggg cttgaaaatc cagacaatca ggtaccagtc
    13141 tgggtcagcc agttgggcca gttccggcga agtggtgctc aacatagggg cggtaatcgc
    13201 ctgtccgaaa gcaaggaaca gaccgagtat gacgcctccc aatatggccc cacgcacgcc
    13261 cccggtagcg ttgccgaaga ctccaccagc accgcctggg aagaagagca tgatcatggg
    13321 cggtacgatg actgcccagc cgatggcacc aaagacgatc atgcagacca tgaatacgac
    13381 gaatgccgag ataaagccga ccagaacggc ggtgggggca taatcgaagg tgatcgggca
    13441 gtcgagggca ggcctggcac cgggcacgat cttcaaggca atgccgcgaa aggccggtac
    13501 gatctcaccc aataccatgc gcacaccggt gagcaagacg gcgatgccga cggcgaaggt
    13561 gaggccttgc agggcagcga agacgacgta gtttagagtt cccgaatcga cctttgttgg
    13621 tccagccacc agagctgcaa tcacggtcac gatggcgatt actaccgcag tggcgactgt
    13681 tacatcgcgg aagaaggtaa gcgacttggg gagttggagt ttctcggtgc tatcgtcagc
    13741 cttacccaca tatttgccca gcgtgccggc gagccagacg ttggttgctg aggtatgccc
    13801 gtaggctagc tcatcggtcc cggtcacctt gcgcatcaga ggctgtatcc acgctggctg
    13861 gatggtccag tatacaccag caatgatcga gccgacgacc accatggcta agacggaggt
    13921 attgggattg atctccagca taacggcgac cagcacaagc gagatccaga acatcaggtg
    13981 accggtcagg tagatgtatt tcaagcgggt gatgcgggca agcacgacgt ggaagaggaa
    14041 tccgaaggtc attatcagag ccgcggtgct cccatggttg cccatcaggg tgcccaagcc
    14101 tgcgcctccg aaagcgtcga acagactttt ttccatattg aaggctgctt gcaggatggg
    14161 tgtaaacttg agcagggcat ctactacaat ttcggcgcct tttgccatga tcaggaaccc
    14221 aacggcggtc ttcaatgtgc cggtgaccac atcactgaag gctttacgct gggcgatcag
    14281 gccgaccatg gcgatcaagc caaccaataa cgatggctgg ctgaagatat tatcagcgat
    14341 gaatttggcg atattgatga tgatatccat atcatactcc ttctgggtga ctttgcgttg
    14401 aaagtgtatc ttggtgcaat atttgtgagg gtcaaaccct ccttacctgg gcatttacac
    14461 aaataacaac gactcaacta atccactcaa agggcgatct gcttcctcac ctccttccac
    14521 catgttttac tcggctacta tcccctctca cttaggaatt cttccaaggc ggcgcgaatc
    14581 tcggccttgt tgacaaagct cttgatggcc ttgacgggcg cgttgccgcc ggaacaaact
    14641 tccaccaagg caggggaggt taggatcaag tctgcgcgcg ctccccgggc ttgtgtggca
    14701 tctgaggctt ctacgtttgc ttctaaccct agttctttaa gcacatcttc ggtgtacatg
    14761 cgcaggagta agctgctgcc catgccgaca ccacaaacgg tgataatctt gagtgacata
    14821 gcgctcctct ctacctttgt tggcgaaatt tgcaggaact gattaatctt gtgtgatatg
    14881 aagggtggtg ctcagttgat aacgatctcc gcggtagact gattctgtgt actcggccac
    14941 ttttccctgg tcggtgtggg taacgcgctt catgcgcaac acagcagcag gagattttaa
    15001 ttgcagcatg cgtaactcct ggcggtttgc taaggcggct tctacagtct gctcggcagt
    15061 cacaagcttc agtccgtaat cctggcgcag gacgctgtag agcgattcat tggagaagtc
    15121 atgctcgaga agatcgggac acagggattg gggtagatga gccttctcgt aggcgatgag
    15181 gtgcccgttg gcgaggcgca agcgtgccag acagatcacg ccgctgttgg gatgtacctg
    15241 gagtatccca gccaactcag cagtggcagg aagaacagca gtgtccatga cacggctgga
    15301 cgtggtcata cccagacttt gcatctcttc gctgaagccg gtcagggtgc gatcctgctc
    15361 gaagcgcacc tcaccaacaa aagtcccctt acca
//
LOCUS       DBNGSX010000287         4916 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_924449, whole genome shotgun sequence.
ACCESSION   DBNGSX010000287 DBNGSX010000000
VERSION     DBNGSX010000287.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_924449"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="AC16CB_08780"
     CDS             complement(<1..356)
                     /locus_tag="AC16CB_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR3342601.1"
                     /translation="MNTVLETLNSALHRAFTDDAHVLLLGEDVLDPYGGAFKVTRGLS
                     TRFPERVLTTPISEAGIVGVAAGMALRGLRPVVEIMFGDFVALAADQFVNHLAKFRWM
                     YNEQVRLPLVIRTPMGG"
     gene            complement(385..1281)
                     /locus_tag="AC16CB_08785"
     CDS             complement(385..1281)
                     /locus_tag="AC16CB_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR3342602.1"
                     /translation="MMENTALYRSLYLIRAFEETVLANFPRGIFFGTTHTYLGQEANA
                     VGVLSHLQAGDVVFSNHRCHGHYLAYGGDPRSLFAELMGKATGVCGGRGGSQHLHWKN
                     FYSNGVQGGIVPIATGMALAEKRKGSGAVTVAFLGDGTLGEGVVYEALNMASLWQAPI
                     LFVLENNRIAQTTPVELAVAGKIPARFNAFGIPARQLDSSDVNEIVPLAFELMKEVRT
                     LSVPRALILHTYRFGPHSKGDDTRNPQDVDRIKQARDPLSIHASRLGPDERAAIEADI
                     KQLIEQAFHQAMVDPFPVISDA"
     gene            complement(1284..1910)
                     /locus_tag="AC16CB_08790"
     CDS             complement(1284..1910)
                     /locus_tag="AC16CB_08790"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR3342603.1"
                     /translation="MFPPGIPLSKRLLDLLLTIPGLVLVSPVLLLIALMVRIIHGKPV
                     LFRQLRPGYRGQPFYVYKFRTMTEDHDEVGNLLPDTHRLTALGRFLRTTSLDELPELF
                     HVLAGKMSLVGPRPLLMQYLDRYTPEQARRHDVLPGITGWAQVNGRNAITWEDKFRFD
                     VWYVDNWSLWLDIKILAVTLWKVLKREGISQPGRATADEFMGSKRDEG"
     gene            complement(1957..>4916)
                     /gene="mfd"
                     /locus_tag="AC16CB_08795"
     CDS             complement(1957..>4916)
                     /gene="mfd"
                     /locus_tag="AC16CB_08795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR3342604.1"
                     /translation="LRPGQNAQPDELARAWSTLGYTHVNTVIAPGQFSRRGGILDIWS
                     PASEHPSRIEFFGDEIETLRQFDPATQRTSGHSERLMITPAREYILPTDLETPPEEIS
                     EFHIPIMHPYPASLLDYLPRQALVLIDDLQAILDATLDLEEQAVTFRKDAIAEGVIPE
                     DFPVPYLSWSEIADNLPADRTMVLGSKTDLETLQDEDSQSGNPISRSFLPGPRYGGRL
                     KPFMEYLTERVEQGENSIIVSRQSARLAELWGESTPYPLPTSPIFIEGSLTEGFTFLS
                     ADSPPVHLFTDGEIFGWRRPELRQRPRAVAESPEGAYTDLQAGDYVVHVDHGIGIFVG
                     LVTRTIDSLEREYLCIEYADEAQLFVPVHQADRVTRYVADTRTPALSRLGGMEWRNVK
                     THVKEAVVEVAQDLLELYATRSVVKGHAFAKDSPWQQELEASFPYIETDDQIRVLDEV
                     KRDMETARPMDRLICGDVGYGKTEVALRAAFKAVMDGKQVAVLVPTTVLAQQHYQTFR
                     QRLAAFPVAVEMLSRFRSHAEQRDIVQRLQNDAVDIVIGTHRLLSSDVVFKDLGLLII
                     DEEQRFGVTHKETLKKLRSEIDVLTLTATPIPRTLYMALTGVRDISTINTPPEERLPI
                     ITHVGPYQPRLVRQAILRELERGGQVFFVHNRVQTIGGISRHLEQLVPEARIAIAHGQ
                     MPEHILSERMKQFVNGDVDVLVSTSIIESGLDIPNANTLIVDRADSFGLAQLYQLRGR
                     VGRGAQRAYSFFFRHTRRSPTPEGRERMETIAENTQLGAGYSIAMRDLEIRGTGDILG
                     TRQHGHIVAVGFHLYTRLLAEAVRQLRPSQPALLQKGWQIPRPSPANVDLPMPANIPA
                     EYVADRTMRLRLYRRMADLQTLQEVDSLADEFVDRFGPLPVAVSNLFFQLKVKLLAEQ
                     ASLASISSENGQLVMRFPEKEVPASLPNPGPFVRVGKTALWMPYTNLPNWRETLVEVL
                     KNLANKDGVAIRE"
BASE COUNT         1170 a         1379 c         1325 g         1042 t
ORIGIN      
        1 ccacccatcg gggtgcggat caccaaaggc aagcgcacct gctcattgta catccagcgg
       61 aacttggcga ggtggttaac aaattggtcg gcagccaaag cgacaaagtc gccaaacatg
      121 atctccacca ccggacgcag gccgcgtaaa gccatccctg ccgcaacacc gacaatgccc
      181 gcctcagaga tgggggtggt taacacgcgt tcagggaagc gcgtggaaag tccgcgcgtc
      241 accttaaaag cccctccata cggatcaagc acatcctcac caagcagcaa tacgtgcgca
      301 tcatcggtga aggcacgatg cagggcagaa ttaagggttt cgaggacggt attcatgggt
      361 agctatgttt caaacagtca cggtttatgc atcgctgatt actggaaaag gatcaaccat
      421 agcttggtga aatgcctgct cgatcaactg ctttatatcg gcttcaatgg cagcacgctc
      481 gtcagggcca aggcgggagg catgaataga taacggatcg cgcgcctgct tgattcgatc
      541 gacatcctgt ggattgcgtg tgtcatctcc cttggagtgg ggaccaaagc ggtaggtatg
      601 cagtatcagc gcccgcggca cgctcaaggt acgtacctct ttcatcaatt caaatgctag
      661 cggaacaatc tcattcacat cgctgctatc taactggcgc gccgggatgc caaaggcatt
      721 gaagcgggca gggattttcc cagcaaccgc cagctcgaca ggagtggtct gggcaatacg
      781 attattctcc agcacaaaca agatgggagc ctgccacagc gaagccatgt tcagcgcttc
      841 atacaccaca ccctcaccca gcgtgccatc acctaagaaa gccacggtca ccgcaccgct
      901 ccccttgcgc ttctccgcca aggccatgcc cgtggcaatc ggcacaatgc ctccctggac
      961 gccgtttgag taaaagttct tccaatgcag gtgctgcgag ccacccctcc caccgcacac
     1021 gccggtggct ttacccatca gctcagcgaa caggctacgc gggtcaccgc catatgctag
     1081 atagtgccca tgacagcgat ggttgctaaa aactacgtct cccgcttgta ggtggctgag
     1141 cacgcccaca gcattcgcct cctgccccag gtaggtatgt gtggtgccaa agaatatgcc
     1201 gcgtgggaaa ttcgctaaaa ctgtctcttc gaaggcacgg atgaggtaaa gggagcgata
     1261 taacgccgtg ttttccatca ttctcaaccc tcgtcccgct tcgaacccat aaattcatcg
     1321 gccgtggcgc gtccaggctg gctgataccc tcgcgcttga gcaccttcca caaagtcacc
     1381 gccaggattt tgatgtccaa ccagagcgac cagttatcca cataccagac atcgaaacga
     1441 aacttatctt cccaagtaat agcattgcgc ccgttgactt gtgcccaacc ggtgatgcca
     1501 ggcagcacat catggcggcg cgcctgctcg ggcgtgtaac gatccagata ctgcatcagc
     1561 aaggggcgcg ggccaaccag gctcatttta cctgccaaga catgaaataa ctccggtagc
     1621 tcatccagac tggtggtgcg caaaaagcgt cctaatgcag tcagccggtg ggtatctggt
     1681 aacaggttgc ccacttcatc gtgatcttcg gtcatggtgc ggaatttgta cacatagaat
     1741 ggctgacccc ggtagcccgg acgtaactgg cggaagagca ctggcttgcc atgaataata
     1801 cgcaccatca gggcgatgag tagcagcaca ggcgagacca acactaaacc agggatggtc
     1861 aatagcaggt caagaagcct ttttgagagg ggtatgccag gtgggaacat gtctcgggag
     1921 gtaaatgata gggataggtg acatgggaga gaagcgctac tctcgtatcg ctaccccatc
     1981 tttattcgcc aaattcttta atacttccac aagcgtttca cgccaatttg gtagattagt
     2041 atagggcatc cacagtgcgg tcttgccaac ccgcacaaat ggccccggat taggtaatga
     2101 cgcaggcact tccttttctg gaaagcgcat cacaagctgt ccattctcgc tgctaattga
     2161 agctaggctg gcttgttctg ccagcaactt caccttaagc tggaaaaaca ggttgctcac
     2221 cgctaccgga agcgggccaa aacgatcaac aaattcatcc gccagggaat ctacctcctg
     2281 cagagtttgg agatcagcca tgcgccggta caagcgcaag cgcattgtcc gatcagccac
     2341 atactcggca gggatattgg caggcatggg gagatccaca tttgctggac taggccgggg
     2401 aatttgccac cctttctgaa ggagagccgg ttgcgaaggt cgcagttgcc ggacagcctc
     2461 ggccagcaat cgggtgtaaa gatgaaagcc aaccgcaaca atatgtccat gttggcgtgt
     2521 ccctaagata tcacccgtgc cgcggatctc cagatctcgc atggcaatcg aatagcccgc
     2581 gcctagttgg gtgttttctg cgatggtttc catccgctcg cgaccctcgg gtgtgggcga
     2641 ccggcgtgtg tggcggaaaa agaaggaata tgcacgctga gcaccgcggc ccacccggcc
     2701 gcgcaattgg taaagctgcg ccaagccaaa gctatcggcg cggtcaacga tgagtgtatt
     2761 tgcgttggga atatccaggc cagactcaat aattgacgta ctcacaagaa catccacgtc
     2821 accgttgacg aattgcttca tgcgctctga aagaatgtgt tccggcattt gcccgtgtgc
     2881 gatggcaatg cgcgcttcgg ggacaagctg ctccaaatgg cgggatatgc cacctatcgt
     2941 ttgtacacga ttatggacaa agaaaacctg cccgccccgt tcaagctcgc gcaagatggc
     3001 ctggcgtacc aaccgtggtt ggtatggccc cacatgcgtg atgatgggca agcgctcttc
     3061 aggaggggtg ttaatcgtgg agatatctcg cacgccggtc aatgccatat acagtgtgcg
     3121 cgggatgggc gtggcagtca gggtgagcac gtcaatttcg gaacgcaatt ttttaagcgt
     3181 ttctttgtgg gtcacaccaa agcgttgctc ctcatcgatg atcaggagcc ccaggtcttt
     3241 gaataccaca tctgaagaca ggagccggtg cgtaccaata acgatatcga cggcatcatt
     3301 ctgcaagcgc tgcacaatat cccgctgctc tgcgtgggag cggaagcgag agagcatctc
     3361 caccgccacc ggaaaggctg ccagcctctg gcggaaggtt tggtaatgct gttgggccag
     3421 taccgtggtg ggaaccagca ccgcgacttg cttgccatcc atcacagctt tgaaggcagc
     3481 gcgtaaagcc acttcggtct tgccatatcc cacgtcgcca caaatcaacc gatccatcgg
     3541 ccgcgcggtt tccatatcgc gcttcacctc gtccagcacg cgaatctggt cgtcggtttc
     3601 gatatagggg aagctggctt ccaattcttg ctgccaggga gaatccttcg caaaggcgtg
     3661 ccccttcacc acactgcgcg ttgcgtaaag ctcaagcaag tcctgggcta cctctaccac
     3721 cgcttctttc acatgagtct tgacattgcg ccattccatc ccccccaggc ggctcagcgc
     3781 cggcgtgcgc gtatcggcga cgtaacgagt gacgcgatcc gcctgatgca caggcacaaa
     3841 caactgcgcc tcatcggcat attcgatgca caggtattcg cgctctaatg aatcaatcgt
     3901 gcgcgttacc aggcccacga aaataccgat gccgtgatcc acatgaacaa cataatctcc
     3961 agcctgcaag tcagtgtatg caccctctgg ggattcagcc actgcgcgcg ggcgctggcg
     4021 aagctccggg cgtcgccagc cgaaaatctc accatctgtg aataagtgca cgggaggcga
     4081 atcagctgat agaaaagtaa aaccttctgt caggcttccc tcaataaaaa ttggagaagt
     4141 tggaagagga taaggagtgc tttcgcccca taactcagcc aggcgagcgc tctggcgtga
     4201 gacaatgatg gaattctcac cttgttccac tcgctcagtc aagtactcca tgaaaggttt
     4261 cagcctgcca ccatagcgtg gtccgggaag aaaactgcgg gatatagggt tgccagactg
     4321 agaatcctcg tcctgcaagg tctccagatc ggtcttgctc cccaagacca tcgtgcgatc
     4381 ggcaggcaag ttgtccgcga tctcactcca gctcaagtag gggacgggga aatcctctgg
     4441 gattacgcct tcggcaattg cgtccttcct gaaagttacc gcttgctctt cgaggtctaa
     4501 agtggcatcc agtattgcct gtaaatcatc gataagcacc agcgcctgac gaggcaagta
     4561 atctaacaag ctagccgggt atggatgcat gattggtatg tgaaactcgg agatttcttc
     4621 tgggggtgtc tccaggtcag ttggcaagat atactcgcgc gctggggtaa tcatcagccg
     4681 ctcgctatgg ccggatgttc gctgagtagc tgggtcaaat tgtcgcaggg tctctatttc
     4741 gtcgccaaaa aactcgattc ggctaggatg ctccgacgct ggagaccaga tgtcgagaat
     4801 accgccgcgg cgggaaaact gacctggggc tatgactgtg ttgacgtgtg tataacccag
     4861 agtagaccaa gcgcgcgcca gttcatcggg ctgggcattc tgtcctgggc gaaggc
//
LOCUS       DBNGSX010000288         3934 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_92985, whole genome shotgun sequence.
ACCESSION   DBNGSX010000288 DBNGSX010000000
VERSION     DBNGSX010000288.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_92985"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="AC16CB_08800"
     CDS             complement(<1..821)
                     /locus_tag="AC16CB_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3342605.1"
                     /translation="MEETITTSTVQEEIYKPRSAQTDRLLRWLTYLLTAFIIILFLFP
                     WLWVFSLGFKTRTEILSPNIVWIWKPTLDNFNEVLLVKGYWRLILNSLLVAASAVLLC
                     MIIGVPTAYAFSRFPIPGKESWFYMILTFRMAPAVVLALPLYVLFARTRLLGTYFAPI
                     IAHITFTLPFVIWLMRSFFEDIPKEIDAAAMTDGYTRAGAFFYAVLPLVKSGIVAVTL
                     FSIIFSWNEFLYGLILTSDTTRPVASQIPSLIRPHGTLWGEVCALATAASLPVAF"
     gene            complement(844..1725)
                     /locus_tag="AC16CB_08805"
     CDS             complement(844..1725)
                     /locus_tag="AC16CB_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3342606.1"
                     /translation="MSSKRYIWWWVLPAAILFLVIILVPFVTALTYSFRALSFTDSTA
                     AGQYIGADNYRKLVFDKDFYNSFKVTFLYMFPAVIVQGLLGLAMSLLISGVVRRARIL
                     IPIMLLPTLLTPVVIGLVGVLTLNPEFGVIGQWLNRSGLIKEAVLGSGKWALPAIIAV
                     DTYQWTPFVAVILTSGLLALPKEPYEAAQVDGANRVQTFFRVTMPLLWPYFVVALLIR
                     VIDAFRVFDIIWVMTRGGPGTVTETVAVYAYRHTFRYWDLGYGSALIMVIFVFISILV
                     EYLYKALQRWQGVSSSA"
     gene            complement(1757..2872)
                     /locus_tag="AC16CB_08810"
     CDS             complement(1757..2872)
                     /locus_tag="AC16CB_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3342607.1"
                     /translation="MASVIIENLFKQYKGGEIQALKGVNLTCDDGEFFCILGPAGAGK
                     TTLIKLIAGIEAADDGKIKIGNQDATDKLPMDRDIAVAFETYALYPQRTVRENLAFPL
                     QAPSRRNEWTKEAIETRVVEIANILGIGELLERYPRQLSGGQRQRVALGRALVRKPQA
                     YLLDEPIAHLDAKLRHRMRGELKRIQMELGITTIYTTPDQLEALSLADKMVVLNQGKI
                     EQFGTPTEIYKYPANVFVARFVGDPPMNILDANLDADGITFQGLGRLPSNETSKRVLK
                     HSANNGNILIGIRPRDLELLPSSAEKSWVTAEVRHVQILGETCIVSFNAGPFDLRSRL
                     ATSQAPERGQKVGVFIDPETFHYFDHQSHQRIDNHVN"
     gene            complement(2905..>3934)
                     /locus_tag="AC16CB_08815"
     CDS             complement(2905..>3934)
                     /locus_tag="AC16CB_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3342608.1"
                     /translation="LSINEGEFFALLGPSGCGKSSTLRMIAGLEKLTKGEIWFDHTLV
                     NELEPRERNIAMVFETYALYPYLNVFENIAFPLRIRGWSKKQIDEKVHWAAEFLGITD
                     LLQMRVRGLSGGQMQSVGIGRAIVREPSILLMDEPISHLEARQRARMREEWVDLQKRL
                     GTTSIYVTHDQVEAMTMATRIAVMNLGELQQVGTPKEVYEQPQTEFVGGFIGEPPMNF
                     TDCDIRQENGGYFAESPSFRVPLGEALGTKLKSYDGSRSIRMGIRPESIRVSMEEMAD
                     SFQASVYVLEPQGERSILTARLSQGEFFLVEVPPNFEADPDTVIYMQFQEPIHLFDKA
                     NGRNLFYV"
BASE COUNT         1051 a          957 c          945 g          981 t
ORIGIN      
        1 aagaatgcca cgggcaggga ggctgcggtt gccagggcgc aaacctcccc ccaaagcgtg
       61 ccatgtgggc gtatcaatga tgggatctga gaggcaaccg gtcgcgtggt atcgctagtc
      121 agtatcaaac catacagaaa ttcattccat gagaagatta ttgagaacaa ggtcacggct
      181 actatgccag atttgacgag tggaaggacg gcgtaaaaga atgcgccagc gcgtgtatag
      241 ccatctgtca tagcagccgc atctatctcc tttgggatat cttcaaaaaa gcttctcatc
      301 aaccagatta cgaacggtaa agtaaatgtg atatgggcga taataggcgc aaaatatgtt
      361 cccaataatc tggtgcgggc gaagagcacg taaaggggca aggctaaaac aaccgctgga
      421 gccatgcgga atgtcaatat catgtagaac cagctctctt tgcccggaat tgggaaacgt
      481 gagaatgcat aggcggttgg cactccaata atcatacata gaagaactgc tgaagcagcc
      541 accaatagag agttgagaat caaccgccag tatcctttca ctaatagaac ttcgttgaag
      601 ttatccaaag tgggtttcca gatccacacg atgtttggac ttaggatttc cgtgcgagtc
      661 ttaaagccaa ggctgaacac ccacaaccag ggaaagagga acaagatgat aataaacgct
      721 gtcagcagat aggtaagcca ccgcaataat cggtctgttt gagcgctgcg gggcttatat
      781 atctcttctt gaaccgtgct tgttgtgatt gtctcttcca tgaggtcact cctttacaaa
      841 ggattaggcg ctgctgctta ccccttgcca gcgctgaaga gctttataga gatactctac
      901 aagaatcgaa atgaaaacaa aaattaccat aatcaacgct gagccatagc ctaagtccca
      961 atagcgaaaa gtatggcggt aagcgtagac tgctacagtt tcggtaactg tccctggacc
     1021 tccgcgtgtc attacccaga tgatgtcaaa cacgcgaaat gcatcaatta cgcggatgag
     1081 caacgcaaca acaaaataag gccaaagaag cggcatggtt acacggaaaa atgtttgaac
     1141 gcggtttgcg ccatccacct gagcagcctc gtatggctcc tttggcaagg caagcaatcc
     1201 agaggtgagg ataaccgcca caaagggtgt ccattgatag gtgtccacag caattatcgc
     1261 aggtagagcc cacttcccgc tgcccaagac agcttccttg atcaatccag agcggttcaa
     1321 ccactggcca ataacgccaa attcagggtt cagcgtgaga acgccaacca gcccgataac
     1381 tacaggggtc aacagcgtgg gaagaagcat gatagggatg agaatccttg cccgccgcac
     1441 gactccagag attaacagcg acatggctaa ccccaataac ccctgaacga tcactgccgg
     1501 aaacatatac aggaaggtta ccttgaagct gttgtaaaaa tccttatcaa agactaactt
     1561 ccggtagttg tcggcaccga tgtactgacc ggctgctgtg gaatccgtaa agctaagagc
     1621 acggaaactg taggtcaggg cggtgacaaa aggtactaag ataatcacca ggaataaaat
     1681 tgccgcaggt aatacccacc accaaatgta acgtttgctg ctcatatgtt agctcctctt
     1741 ctacaaagat ggtgcgctag ttaacatgat tgtcaatccg ctggtgggat tgatgatcga
     1801 aatagtgaaa tgtctctggg tctatgaaaa ccccaacctt ctgaccccgt tcaggagcct
     1861 ggctagtggc taagcgtgag cgtaaatcaa atggtccagc attaaacgaa acgatgcatg
     1921 tctctccaag tatttgaacg tgtcttactt ctgctgttac ccaacttttc tctgcgctcg
     1981 agggcaatag ctcaaggtcc ctgggacgga taccaattaa tatatttcca ttgttggcgg
     2041 aatgtttcaa aactcttttc gaagtttcat ttgatggcag gcggccaagc ccctggaagg
     2101 tgatcccatc cgcatccaga ttagcatcca atatattcat tggaggatcg ccaacaaaac
     2161 gagcaacgaa gacattggct ggatatttat agatttcagt gggggtgccg aattgctcaa
     2221 tttttccttg gttaagtaca accatcttgt ctgccagaga taaggcttcc aattgatctg
     2281 gggttgtgta gatcgtggtg atacctaatt ccatttgaat gcgtttgagc tcgccacgca
     2341 tgcgatggcg caattttgca tctaaatgag cgatgggctc atcgagaaga tatgcctgag
     2401 gtttgcgtac cagggcgcgc cctaaagcta cccgctgtcg ttgcccgccc gataattggc
     2461 gcgggtagcg ctcaagtagc tcaccaatcc ccaggatatt ggcaatctct accacgcgtg
     2521 tttctatagc ctcctttgtc cactcatttc tgcgtgaagg tgcttgcaaa ggaaacgcaa
     2581 gattctcgcg aacagtgcgt tgtgggtata gggcataggt ttcaaaagcc accgcgatgt
     2641 cccggtccat gggtaatttg tcggtagcat cctgattgcc gatcttgatt ttgccatcat
     2701 ccgctgcttc aatgcctgca atcagcttaa ttaaggtggt tttcccagca ccagcgggtc
     2761 ctaatataca aaaaaattcg ccatcatcgc aggtcaggtt gacacctttt agcgcctgga
     2821 tttcgccacc tttatattgc ttaaataagt tctctataat aacgcttgcc atctaatcct
     2881 ccgtgccata cggaataagc ctgctcagac gtagaacagg tttcttccat tggctttgtc
     2941 gaataagtgg atcggctctt gaaattgcat gtagataacc gtatctgggt cagcctcgaa
     3001 atttggaggg acttcgacca ggaaaaattc gccttgactc aagcgagccg tcagaatgga
     3061 tcgttctcct tggggttcta agacataaac gctggcctgg aaactatcgg ccatttcttc
     3121 catgctgacg cgaatactct ctggacgaat gcccatccgg atggacctgc tgccatcata
     3181 agattttagt ttcgtcccca atgcctcgcc tagagggact ctgaaggatg gcgactcggc
     3241 aaaataaccg ccattttcct ggcggatatc acaatccgtg aagttcattg gtggctcgcc
     3301 aataaagccg ccgacaaatt cggtctgggg ttgctcgtat acctctttgg gtgtgcccac
     3361 ctgttgtaat tcgcctaaat tcatgacggc gatgcgcgtc gccattgtca tggcttccac
     3421 ttgatcgtgg gttacgtaga tagaggttgt ccccagtcgc ttttgcagat cgacccattc
     3481 ttcgcgcatg cgagcgcgtt gtcttgcttc taggtgtgag atgggctcgt ccattaacag
     3541 gatcgatggt tcacgcacga tggcccgtcc aatgcctaca ctttgcattt gaccgccact
     3601 caatccgcga acccgcattt gcaacagatc agtgatacct aaaaattctg ctgcccaatg
     3661 aactttctcg tcgatttgtt ttttgctcca gcctcgtatt cgtagtggaa aggcgatgtt
     3721 ttcgaaaacg tttagatatg gatagagagc gtatgtctcg aaaaccatag cgatgtttcg
     3781 ctcgcgcggc tccagttcat taactaaggt atggtcaaac caaatttcac ccttcgttaa
     3841 cttttccaag cctgcgatca tgcgaagtgt ggatgatttt ccacacccgg aaggaccgag
     3901 cagagcgaag aattcccctt cattaatcga gagg
//
LOCUS       DBNGSX010000289         3671 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_715883, whole genome shotgun sequence.
ACCESSION   DBNGSX010000289 DBNGSX010000000
VERSION     DBNGSX010000289.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_715883"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..994)
                     /locus_tag="AC16CB_08820"
     CDS             complement(119..994)
                     /locus_tag="AC16CB_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009663273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR3342609.1"
                     /translation="MATTLRFIIIPKVQHPWFDEVNNGAQAQAEILSRALGAEIVVDY
                     MPPSICDIVEQNAILENAAKSRPSGIAIDPVDVVDHMTAINRVRDQGIPVVLFDSASP
                     DGSITSVGNNFAQQGVIAAERLAKLIGYTGKVAVMQGYPTAPNHKERYEAQVEVLKKY
                     QNITALDGGIDNDDIETARQQASSVLESHPDLSGYLCCDASGPIGIATAIKEAGKAGK
                     VKVVSMDGIKPILDAIKQGVIDSSSATIPKMQGSMSVLMLWQASLGVQMPQAIDTGIA
                     VITQENVDRYLADAM"
     gene            complement(1234..1818)
                     /locus_tag="AC16CB_08825"
     CDS             complement(1234..1818)
                     /locus_tag="AC16CB_08825"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3342610.1"
                     /translation="MSVPVVTTANESQRQSAIAVITLAFAHDPLARWALPDAVNYLAA
                     MPQFIDAFGGNGLAHGTTHLANGGSGAAMWLPPGLQPNEERMISIMQQYVAGQTLEEL
                     LAVFEGMGKYHPDDPHWYLPLIGVDPAYQCLGIGAAMLRHALQRCDADRALAYLESSN
                     PRNIQLYERHGFEALGVIQVGGSPQVVPMLRRPR"
     gene            complement(1834..2106)
                     /locus_tag="AC16CB_08830"
     CDS             complement(1834..2106)
                     /locus_tag="AC16CB_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3342611.1"
                     /translation="MARAKRSIGLEILEGIREIKRGEHGRVTTIPSVTSVREKTGLSQ
                     SRFAQLLGVSVRTLQEWEQGRRAPSGAARTLLLIAQKNPRALLEVA"
     gene            complement(2099..2410)
                     /locus_tag="AC16CB_08835"
     CDS             complement(2099..2410)
                     /locus_tag="AC16CB_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR3342612.1"
                     /translation="MFSFVESKLFTRLVGDYLTDDEYRRLQATLIEAPESGAVVPGSG
                     GVRKLRWAQPGRGKRGGIRVIYYAKTREGVIWMLTIYAKNEAQNVPAHVLRKIKEELD
                     G"
     gene            complement(2543..3418)
                     /locus_tag="AC16CB_08840"
     CDS             complement(2543..3418)
                     /locus_tag="AC16CB_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342613.1"
                     /translation="MRHHRHLAFGLAATVMLSGLANAQSPTSADALAFHARVVALYSF
                     EPHKLTSEQIEAKSKVLDSFWEEVKADPKRYLPLLRNELRDGGNSAFFSYDGSKLLLA
                     VSKERKDQTLALEAIPRADLQGVQHTDYLRTVHWFARNGFNTTKAALRILDYPDFKAF
                     IVQHALTLGQDYSLIYMLFPMPDASYVGPLIDRLNIEDKLVSQKSILLALWYSMTPEA
                     KAAAARFIADTSKSQDARNYAKELSERKAPLTSVLTLSTTRQLKEERQKVMYRLSDEA
                     LYEFESLTAKLLAKQ"
BASE COUNT          746 a         1019 c         1080 g          826 t
ORIGIN      
        1 gaacgcagcg tagcggagcg gaggagtaag gcgccggcct cgaagcgctt gttaggcacg
       61 cgccttgcac cctcaattgc caaattgtgt tgaggacctg gcatgggata tcgggccttt
      121 acattgcatc agccagatat ctatccacgt tctcctgtgt aatcactgca atacccgtat
      181 caatagcctg aggcatctga acgcccagcg atgcttgcca caacatcagg accgacattg
      241 agccctgcat tttcggaatg gttgccgagg aggaatcgat gacgccttgt ttaatggcat
      301 cgagtatggg cttaatccca tccatgctga ccactttaac tttgccggcc ttcccggcct
      361 ctttgatagc ggttgctatg ccgatcggac cggaagcatc acagcacaag taaccactta
      421 gatcgggatg cgattcgaga acagacgagg cttgttggcg agcagtctcg atgtcgtcat
      481 tgtcaattcc accgtctaga gcagttatgt tttgatattt tttcaatact tctacctggg
      541 cttcgtatcg ttctttgtgg tttggtgcgg tgggatatcc ctgcatcaca gccactttgc
      601 cagtgtaccc aatcagcttg gcgagacgct cggccgctat gaccccctgc tgagcaaagt
      661 tgttgcccac gctggtgatg ctgccatccg gcgatgctga gtcaaagaga accacgggaa
      721 taccctgatc ccggacgcgg ttgatggctg tcatgtggtc cacaacgtca accggatcta
      781 tggcgatgcc actcgggcga ctcttggcgg cattctccag gatcgcgttc tgctcgacga
      841 tgtcgcaaat cgatggcggc atatagtcga cgacgatctc ggcacccagc gcccggctta
      901 ggatttcggc ttgagcctgg gcaccgttat ttacctcgtc gaaccagggg tgttggactt
      961 tcgggatgat aatgaatcga agcgttgttg ccattttttg ggccctctca ggggtagcgt
     1021 aaaagcctaa cgttcgagct gagccgcccg cggaggcggg cagcgcttgg ccgcggaacg
     1081 acaatgacca cggtggcctg gagtggccaa gcgctgcctg ccgtagcagg tcggctcgag
     1141 cgacgggtta ggccgcgcct ggcccctgtc gtatgaaagc cgcaaaggga gcatcgcgtg
     1201 taatcgaatg cagggatcgc aaagttacgc ggatcagcgc gggcgtctca gcattggaac
     1261 cacttgcggt gatccgccca cctggatgac gcccagtgcc tcgaatccgt gtctctcata
     1321 gagttggatg ttgcgtgggt tggatgattc caggtaagca agtgcacgat ccgcgtcaca
     1381 gcgctgaagg gcatggcgca acatcgccgc accgatgccg agacactggt aggcaggatc
     1441 aacgccgatg agtggtaggt accagtgagg atcgtccggg tgatactttc ccatcccctc
     1501 gaacacagcc agcaactcct cgagagtttg tcccgcaacg tactgctgca ttatgctaat
     1561 catgcgttct tcgttgggct ggaggccggg tggcagccac atcgccgcac cactgccgcc
     1621 atttgcgagg tgggttgtgc cgtgggccaa gccgttgccg ccaaaggcat cgatgaactg
     1681 gggcatcgcc gccaggtaat tcacggcgtc ggggagagcc catcgcgcta gggggtcgtg
     1741 ggcaaatgcg agcgtaatta cggcaatcgc ggattggcgc tgtgactcgt tggcggtggt
     1801 tacgactggc acgctcatga cgttagatta cggttaagcg acttccagaa gcgcacgcgg
     1861 gtttttctgt gcaattagga gaagcgtacg ggcggcacct gatggagcac ggcggccctg
     1921 ctcccattcc tgcagagtac gcacagagac cccaagaagt tgggcgaacc gggactgcga
     1981 gaggccggtt ttctcgcgca cggatgtcac tgatgggatg gtggtcaccc ggccatgctc
     2041 gccacgcttg atttcgcgaa ttccttcgag aatctcaaga ccgatactgc gtttggccct
     2101 agccatcgag ttcctccttt atcttgcgaa gaacgtgagc cgggacgttc tgcgcttcat
     2161 tcttcgcgta aatcgtcaac atccaaatca cgccttcgcg ggtcttggcg tagtagatca
     2221 ctctgatgcc gccccgcttt ccgcgcccgg gctgcgccca ccgcagcttg cggaccccgc
     2281 ctgaccctgg aactaccgcg ccactttctg gcgcttcgat cagtgttgcc tggagccgtc
     2341 ggtactcatc gtcggtgagg taatcgccga caaggcgcgt aaacagcttt gactcgacaa
     2401 agctgaacat ggcacattat acgtcagtga cggatagcgt caaccggaat ctaacgttca
     2461 cgcccaccgg cgcgcgcccg ctgacttttg cactgcacgg cgctggtgcg cgcgtccggg
     2521 tcgggcgtgc tgttaggcgt tttcattgtt tggcaagcag cttggcggtc aagctttcaa
     2581 actcgtatag cgcctcgtca cttaatcgat acatgacctt ctggcgttcc tccttcaact
     2641 ggcgagttgt cgacagcgta agaacactgg tcaaaggtgc ctttcgttcg gaaagctcct
     2701 tagcgtagtt ccgagcatct tgcgatttgg aggtgtccgc aataaagcgc gcggcggccg
     2761 ccttggcctc aggggtcatt gagtaccaaa gcgccagaag aatgcttttc tgagacacca
     2821 gcttgtcttc aatattcagg cgatctatga gtggccccac gtagctggcg tccggcatag
     2881 ggaacaacat gtagatgagc gaatagtcct ggccgagtgt aagcgcgtgc tggacgatga
     2941 acgctttaaa gtctggatag tcgagaatgc gtagcgcggc cttcgtggtg ttgaagccat
     3001 tcctggcaaa ccaatggacg gtgcgcagat agtctgtgtg ttgaacgcct tgaaggtcag
     3061 ctcgcggaat tgcctcaagc gcgagagttt ggtctttccg ctctttcgaa acagcaagca
     3121 aaagcttgct cccatcgtac gaaaagaacg cagaatttcc accatcccga agctcgtttc
     3181 ttagcaacgg gagataccgc tttgggtccg cctttacctc ttcccagaag ctatcaagaa
     3241 ctttggattt agcttcaatc tgctccgacg tgagcttgtg aggctcaaag gaatagaggg
     3301 caacgaccct cgcgtggaag gctagcgcgt cggccgacgt aggagattgc gcgttcgcca
     3361 agccagagag cattacagtc gcggcaaggc caaaggccag gtggcggtgg tggcgcatgc
     3421 aaaaacgcct aacgtcgtcg tgaacggcgg gccgctacga ggcggacgtg cggcacggtg
     3481 cgtccggccc atccgttcga cgtgaagtta gaacgcagga cagggacacg ctttgcgccc
     3541 ttgacgtctg cggttactta tcccgcgttc cggcaccgcg agacttctcg gcgtcctgct
     3601 tcgtttgcgt ctctcccggt ttctggaaat cgccaatgac gcttttcaga gggcagattg
     3661 cgactgctgg a
//
LOCUS       DBNGSX010000290         7036 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1824191, whole genome shotgun sequence.
ACCESSION   DBNGSX010000290 DBNGSX010000000
VERSION     DBNGSX010000290.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7036)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1824191"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /locus_tag="AC16CB_08845"
     CDS             <1..1308
                     /locus_tag="AC16CB_08845"
                     /inference="COORDINATES: protein motif:HMM:NF025065.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR3342614.1"
                     /translation="EKSQGEVLVVMDPTPMRRGSDSLKPLVLDVLRNAGWQPNTLVLE
                     PDSSGQVHTDMPHIEWVKARIQASSPVVSVGSGVVTDIAKHACYLYEKETGIAIPYVV
                     YQTANSVSAYTSDMAPVFIEGVKRTLPSRYPDALISDLETLRDAPREMTVAGVGDLLA
                     AFVSFPDWYLAHQLGLDPKYTELPHALMDGMEELFLSNAEEIRLRSLPGMALLAKLIH
                     LAGLAMSLSHATTPLSGYEHVMSHILDLHNEQAGMPLAQHGSQVALTTILGSMAYEAF
                     LDQFEPAEVVVERCFPNAERIRLHILNEFAKIDPSGKAGEECWSDYRVKLERWSEQGS
                     SLEGILANWPQIKQRLQTLARPHETTLRILQAVEAPTNFEALIPPISESSAKFAFFNA
                     PLMRYRFTLGDMLLFLNWDREALWERIWKSFSPPPSPPRPFSS"
     gene            complement(1268..4900)
                     /gene="smc"
                     /locus_tag="AC16CB_08850"
     CDS             complement(1268..4900)
                     /gene="smc"
                     /locus_tag="AC16CB_08850"
                     /inference="COORDINATES: protein motif:HMM:NF014516.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIR3342615.1"
                     /translation="MMTEDKIALLMRLKSLELQGYKTFASQTVFEFAGLITAIVGPNG
                     SGKSNVADSLRWVLGEQSYSLLRGKKTADMIFSGSESRPRAGMASVTVVFDNSDGWLP
                     IDFSEVSITRRAYRDGQNEYLVNGQRVRLKNVNELLGQSGLAERTYTIIGQGVVDAAL
                     SLKAEERRRLFEEAAGIGLYRARREEAQRRLETTRRNLERVQDILAELSPRLRSLERQ
                     AKRAREFEQIRADLQVMLREWYGYHWHLAQGELREARDLARQRESKLEQARKSYQELD
                     EQIASKRSQLQGVRARLNDWHRQIALLHTRREELSTQTAVVEERLRSWQEQRRNAQVE
                     LVRLQEEMELDRERLLAAEKDVERLNTELSEARAQAEEALSVLQKHQKERANAEGVVL
                     ASRQALAALNRSQGELQARLTERRALAERQSGELSEAEIALEAANNEISLAQKRASES
                     QTAYSRTEFDRAEAEKALQDQRQRRINAEASLKALQEEGAALSAELARLKAQMDVIEQ
                     AETALSGYARGAQALIKSVRQGRLKGAKGALNTFLDVPAELETAIAAALGEFLDSLVL
                     DGVAGIDLGLSILHEESARGILLPLEAISPPAEIRVEDDDPNLIGVASRLIQTPSELR
                     SVVNLLLGRTLVVKNREVAREFLEKYRETDGLRVVTLQGDVFLSGGPVLAGGEATQVT
                     LARPRQRKSLGDRIVSLQKQVTLHDLRQREGESEVERLLGEEIKLEKALQEARQVEAK
                     AIATYNQDAMVVDTLKQQIKWHTERVNRLTEEINHANSEAADLMAEHSKVEEKITLAR
                     EEVRRNESRVGELSLDEYQSQVGFWKTQVAVAERALADVQTRFDERVSRVDDTQNSLD
                     AASHRMVELDDSFKELETEKANLKQAAADVSQQIDALHIQIEPAETELEQMESDQDTI
                     LDTETAARQELNKEEHLHAQAKIAMARKKEALESWRRRIEDDFGLVAFDYAEEVSGPT
                     PLPLSGMVERLPRIKSLSPDMEENVRRLRAQLRRMGPINQEAQDEYHEVKQRYEFMTE
                     QVSDLQQAEADVQQVIVELDELMQRELRRTFDAVAEEFSEMFTRLFGGGSARLLLTDP
                     EDMSSTGVDIEARLPGKRMQGLSLLSGGERSLTATALIFALLKVSPTPFCLLDEVDAM
                     LDEANVGRFRELLRELSRNTQFVVVTHNRSTVQVADVIYGVTMGRDSTSQVISLKMDE
                     VEKVVD"
     gene            complement(4890..5618)
                     /gene="rnc"
                     /locus_tag="AC16CB_08855"
     CDS             complement(4890..5618)
                     /gene="rnc"
                     /locus_tag="AC16CB_08855"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIR3342616.1"
                     /translation="METAQGSLRGESPQDLIQRLGLSITDFGLIARALTHRSFANEHN
                     DVLEDNERLEFLGDAVLDFLVGAWLYDHYPELKEGDLTRLRSGLVRTEQLAEFAREID
                     LGRAMRLGRGEDDGGGRERIALLCGTFEALIGALYLSDGLPLVQQFVEPMLIPAAERI
                     LVSLEEQDPKSLLQEWIQAQGYDHPTYRTVTANGPDHAKIFEVEVIFNGEIQGRGLGH
                     SKQAAAKSAAREALTNLGIIRNDD"
     gene            complement(5782..7020)
                     /gene="fabF"
                     /locus_tag="AC16CB_08860"
     CDS             complement(5782..7020)
                     /gene="fabF"
                     /locus_tag="AC16CB_08860"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR3342617.1"
                     /translation="MAERIVVTGMGAISPLGHSANESWENAIHGVSGVGPITQFDTSD
                     YLVKIACEVKDFRPEDYMEAREARRRDRFTQLAAVAAQEAMRQSGLDVKAEKPERIGL
                     IISSAIGGLKTLQDSIFTLKEDGPRRLSPFVIPMLMSNGAAGMLAIEYGMTGPSFSVA
                     SACASGADGLGTAWMMLRSGMIDVALTGACESTITPIGIGAFDRLGAMSRRNDDFSMT
                     PQPFDKNRDGLVMGEGAAVLVLESEAHARARGAEILAELAGYAATADAFHITAPAEDG
                     AGGALAMKLALNASGVNPEDVDYINAHGTATPLNDISETRAIKSAFGKLAYNIPVSST
                     KSMTGHMMGATGALEATFCVQAVRNNVIPPTIHYQTPDPECDLDYVPNQAREMTVNVA
                     ISNAFGFGGHNAVLVVKKYR"
BASE COUNT         1615 a         1982 c         1691 g         1748 t
ORIGIN      
        1 gaaaaatccc agggagaagt gttggtggtg atggatccaa ccccgatgag gcgtggatct
       61 gacagcctga aacccctggt actggatgtt ttgcgaaacg ccggctggca acccaatact
      121 ctggtgctcg aaccagattc aagcggtcag gtacacactg atatgcccca catcgagtgg
      181 gtaaaggcac gcatacaagc cagctctcca gtggtgtctg ttgggtcagg ggtggtgacc
      241 gatattgcca agcatgcctg ttacctttac gagaaggaaa ctggaatagc catcccatac
      301 gtcgtctacc aaacagccaa cagtgtgagc gcctacacgt ctgacatggc accggtattc
      361 attgaagggg tgaagcgaac cttgccatct cgctatccgg atgcgctaat cagtgatttg
      421 gaaactttac gcgatgcccc gcgtgaaatg accgtggctg gtgtgggtga tttgctggct
      481 gcctttgtga gcttccccga ctggtactta gcccatcaat tgggactgga tccaaaatat
      541 actgaattgc cccatgcatt gatggatgga atggaagagc tctttttgag taacgccgag
      601 gagatacgcc tgcgctcctt acctggtatg gcgttgctag ccaaactcat ccatctcgcc
      661 gggctggcaa tgtcgctctc tcatgcgacg acgcccctct caggatacga gcatgtgatg
      721 tctcacattc tggacctgca caatgagcag gccggaatgc ccctggctca gcacggtagc
      781 caggttgcgc tgactaccat ccttggcagt atggcgtatg aagcttttct tgatcaattt
      841 gaacctgcag aagtggtcgt tgagcgctgt tttcccaatg cggagcgtat aaggcttcac
      901 atcctgaatg aatttgctaa aatcgatcca tccggcaagg ctggggaaga atgctggtca
      961 gattatcgcg tcaaactcga aaggtggagt gagcaaggca gcagtttgga gggcatactg
     1021 gctaattggc cgcaaatcaa gcagaggctt caaactctgg cgcgccctca cgagacgacc
     1081 ctgcgcatcc ttcaggcagt tgaagccccg acaaatttcg aggcattaat tccccccata
     1141 tcagaaagct cggcgaagtt tgccttcttt aatgccccgc tcatgcgcta tcgatttact
     1201 ctgggcgata tgctgctctt tttgaattgg gatcgagaag cgttgtggga aaggatatgg
     1261 aaatccttta gtccaccacc ttctccacct cgtccatttt caagctgatc acctggctgg
     1321 ttgaatcgcg ccccatcgtg acgccatata tcacatcagc gacttgaaca gtgctgcggt
     1381 tatgtgtcac gacgacgaat tgtgtatttc ggctgagctc tcgcagcaat tcacggaagc
     1441 gtcccacgtt agcctcatcc agcatcgcgt caacctcatc cagcaggcag aatggtgttg
     1501 gcgatacttt gagcaaggca aaaatcaatg cggttgctgt caagctacgc tccccacccg
     1561 agagcagaga aagtccttgc atgcgcttac ctggtaagcg cgcctcgatg tccacacccg
     1621 tgctggacat atcttctggg tccgtaagca acaggcgagc agaaccaccc ccgaaaagac
     1681 gtgtaaacat ttcggaaaac tcttcggcga ctgcgtcgaa agtacggcgc agctcgcgct
     1741 gcatcagctc gtctagctcg acaatgacct gctgcacatc cgcttcagcc tgctgcaaat
     1801 cgctgacttg ctccgtcata aattcatagc gctgcttgac ttcatgatat tcatcttgag
     1861 cctcctgatt gatagggccc attcggcgaa gctgtgcgcg caggcggcgc acattttcct
     1921 ccatgtctgg cgagaggctt ttgatgcgag gcagacgctc gaccatacca ctcaagggca
     1981 gtggggtagg tccagaaacc tcttcggcat aatcgaaggc aaccagacca aaatcatctt
     2041 cgatgcgacg cctccaggac tctaaggcct ctttcttgcg ggccattgca atcttggctt
     2101 gggcatggag atgttcttcc ttgttcagtt cctggcgggc agctgtctct gtgtctagga
     2161 tggtatcctg atcactttcc atttgttcca gctccgtctc tgctggctcg atctggatat
     2221 gtagggcatc aatctgttgg gatacatccg ctgctgcttg ctttaagtta gccttttctg
     2281 tttccaattc tttaaacgaa tcatccaact caaccattcg atggctggca gcatccaaag
     2341 agttttgtgt atcgtccacc ctggagacac gctcatcaaa acgagtctgg acgtcagcca
     2401 gtgccctttc ggcaacagct acctgagttt tccagaaacc tacctgagat tgatactcgt
     2461 ccaggcttag ttcaccaacc cgactctcat ttcgtctgac ttcttctcga gcaagcgtaa
     2521 ttttctcttc gaccttagaa tgctctgcca tgaggtcagc tgcttcactg ttcgcatgat
     2581 tgatttcttc ggtcaggcga tttactcgct cggtgtgcca tttgatctgt tgtttgaggg
     2641 tatctacaac catcgcgtcc tgattgtagg tagcgattgc cttggcttct acctggcgcg
     2701 cctcttgcaa tgctttctct aacttgattt cttcacccag caagcgctct acttcgcttt
     2761 ccccttcgcg ttggcgtaaa tcgtgaagtg taacctgttt ctgcaaagag acaattcggt
     2821 ctcctaagct cttccgctgg cgagggcgag ccaaggtaac ctgtgtggcc tcgccacccg
     2881 ccaaaactgg ccctccacta agaaatacat ctccctggag agtaacaact cgcaaaccat
     2941 cagtctctcg atacttctcc aaaaactcac gagcaacctc acgattcttt acaaccaggg
     3001 ttcgacccag caataaatta accacagatc gcaattctga aggcgtctgt atcaaacgag
     3061 aggctactcc aatcaaattt ggatcgtcat cctccactct gatctctgcc ggtggagaga
     3121 tggcttctaa gggcagcagg atgccgcgtg cactctcttc gtgcaaaata gacagaccca
     3181 aatcgatacc agcaacacca tcgagcacaa gagaatctaa aaattcgccc aaagcagcag
     3241 caatagccgt ctccagctct gctggcacat ccaggaaagt gttcaatgcg cctttggcgc
     3301 ccttcaggcg gccttgacgt acagacttga tcagcgcttg cgcgccgcgc gcatagccac
     3361 ttaatgcagt ctcagcctgc tcgatgacat ccatttgagc cttcaaccga gctaattcag
     3421 cagagagagc ggcgccttct tcctgtagcg ctttcaagga tgcctctgcg ttgatgcggc
     3481 gctggcgttg atcctggagc gctttttctg cctctgcacg gtcaaactct gtacggctat
     3541 acgcagtctg gctttcgctt gcacgttttt gtgccagcga tatttcatta ttcgcagctt
     3601 ctaatgcgat ctcagcttct gaaagctcgc cagattggcg ttcggccagc gcccgcctct
     3661 cggttagacg cgcttgaagc tccccctggc tgcggttgag ggcggctaat gcctgccgag
     3721 atgcaagcac aacgccttca gcgtttgccc gctctttttg atgcttttgc aacactgata
     3781 aggcttcttc cgcctgagca cgtgcttcag acaactctgt gttcagccgt tccacatctt
     3841 tctcggcggc gagcaatcgc tcacggtcaa gctccatttc ctcttgcaaa cgtaccagtt
     3901 caacctgtgc attccgacgc tgttcctgcc aagatcgcaa gcgctcttcg acaaccgcgg
     3961 tctgagtgct caactcctcc cggcgagtat gcaatagtgc tatctggcga tgccagtcgt
     4021 ttaggcgggc gcgcacgccc tgaagttgag atcttttgga tgcgatttgc tcatccagct
     4081 cctggtatga tttgcgtgct tgctcaagtt tactttctcg ctggcgagcc agatctcgcg
     4141 cttcgcgcag ctccccttgt gccaggtgcc agtgatagcc ataccactct cgcagcatca
     4201 cttgaagatc agctcgaatt tgctcaaatt cacgcgctcg ctttgcctgt cgctccaggc
     4261 tacgcagtcg aggtgatagt tccgccagaa tatcctgaac gcgctccaga tttctccgag
     4321 tagtttccag acgcctttga gcttcctcgc ggcgagcgcg gtagaggcca attcctgccg
     4381 cttcttcaaa taagcggcgt ctttcttcgg ccttgagcga tagcgctgca tccaccacac
     4441 cttgcccgat gatggtgtaa gtgcgttccg ctaaacccga ctgacctaat aactcgttca
     4501 cattcttcaa gcggacacgc tgcccattaa ctaaatattc attttgacca tcgcggtagg
     4561 ctcgacgcgt gattgagacc tcgctgaagt ctattggaag ccagccatcg ctattgtcaa
     4621 agacgaccgt caccgaggcc ataccggcac gagggcggga ttctgatcca gaaaagatca
     4681 tatcagcagt cttcttgcca cgcagcaagc tgtaagactg ttcccccaac acccatcgca
     4741 ggctatctgc gacattggat tttccagacc catttgggcc tacgatagcg gtgatcagac
     4801 cggcaaactc aaaaactgtc tggctagcaa aggttttata accttgcagc tcgagggatt
     4861 tcagacgcat caggagtgct attttatctt cagtcatcat tccgaattat tccaagattg
     4921 gtaagcgctt cgcgagcagc ggatttcgca gcggcttgtt tgctatgacc caaaccgcgc
     4981 ccctgaattt cgccattaaa aatgacctcc acttcgaaaa ttttagcatg atccggtcca
     5041 ttggcggtaa cggtgcgata ggtggggtga tcataccctt gagcttgaat ccactcttga
     5101 agtaagctct tagggtcttg ctcttcgagg gagacaagga tcctttcagc ggcgggtatc
     5161 agcatgggtt caacaaattg ctgaacaagg ggaagaccat cgctcagata tagtgcccca
     5221 atcagcgcct caaacgtacc gcacagtaat gcaattcgtt cacggcctcc gccatcatct
     5281 tcaccgcgtc ccaggcgcat cgccctaccc aaatcaattt cgcgggcaaa ttcagctaat
     5341 tgttcagtgc gcaccaatcc cgaacgcaat cgtgttaaat cgccttcctt taactcaggg
     5401 taatgatcat atagccaggc gcctactaaa aaatcgagga cggcatcgcc taaaaattct
     5461 aggcgctcgt tgtcctccag tacatcgtta tgttcgttgg caaatgagcg gtgcgtgagc
     5521 gcacgcgcaa tcaaaccgaa atcggtgatt gacaacccca aacgttggat taaatcctgg
     5581 ggggattcac cccgtaggga tccctgagca gtttccatgt ataacctccc ggaactcagg
     5641 gagcgtcaga cgccccacgc cgggtcgagc gtctggcgtt ccattagttc cttctttcta
     5701 cctattgcag atttttgacg cgtggcaggt tgaacctaat aattgtactt ccaaccaacc
     5761 acatctcaca taaagacact ctcaacgata ctttttgacc accaacactg cgttgtgacc
     5821 gccaaaacca aaggcattac ttatagctac attaacagtc atctcccgag cttggttagg
     5881 aacataatcc agatcgcatt cgggatcagg agtttgataa tggatcgttg gagggatgac
     5941 attgttgcgc acagcttgta cacaaaacgt ggcttccagc gcgccagtag cacccatcat
     6001 gtgtccggtc attgatttcg ttgacgaaac agggatatta tacgccaatt taccaaacgc
     6061 ggacttaatc gcgcgggtct cagagatatc atttaatggt gttgcagtac cgtgggcatt
     6121 gatgtaatcc acatcttcgg gatttactcc acttgcattc aaggcaagct tcatcgccag
     6181 tgcaccacct gcgccatcct ctgctggagc ggtgatatga aaggcgtcgg ctgtagccgc
     6241 ataacctgcc aactcagcca atatctccgc tcctcgagcg cgggcatgag cctcggattc
     6301 caataccagg acagccgcac cctcacccat cactagacca tcacggtttt tatcaaaggg
     6361 ttgtggtgtc atagaaaagt cgtcatttcg gcgtgacatg gcacctaaac ggtcaaatgc
     6421 ccctattccg atgggcgtga tagtggactc gcaggcgccc gttagtgcaa catcaatcat
     6481 gccagagcgc aacatcatcc atgcggtacc gagcccatct gcaccagagg cgcaggcaga
     6541 agccaccgag aaggaaggac cagtcatacc gtattcaatc gctagcatgc ctgccgcgcc
     6601 atttgacatc aacatcggta taacaaacgg gctcaggcgg cgggggccat cctcttttaa
     6661 tgtaaatatc gaatcctgaa gagttttcaa gccgccgatc gccgaggata tgatcaaccc
     6721 gatgcgctca ggtttttccg ccttaacgtc cagcccagat tggcgcattg cttcctgagc
     6781 agctacggca gccaattgtg tgaagcgatc gcgacgacgc gcttcacgtg cctccatata
     6841 atcctcagga cgaaagtctt tgacctcgca tgcgatcttg accaaataat cgctggtatc
     6901 aaattgggtg attggtccga cacccgagac gccatggata gcgttctccc atgactcatt
     6961 cgccgaatga cctaacgggc tgattgctcc catccccgtg acgacaatgc gctctgccat
     7021 tatgatgtct ccttag
//
LOCUS       DBNGSX010000291         3041 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_370006, whole genome shotgun sequence.
ACCESSION   DBNGSX010000291 DBNGSX010000000
VERSION     DBNGSX010000291.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_370006"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="AC16CB_08865"
     CDS             complement(<1..1027)
                     /locus_tag="AC16CB_08865"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3342618.1"
                     /translation="MTTLSQLMGRYQLMQELGKGGMGMVYEGHDNLLNRQVAIKVLSE
                     ARNIRLGSEGRSRLLHEAQAAAGLNHPNIVDIYDAGEDQGVSFIVMELVKGRSLHEHK
                     PKSIDEIISIACQICAALDHAHTHGIVHRDLKPENVLVTPDGTAKLTDFGLARSVASH
                     ISLDGMIVGTVYYLAPEAALRQSYDGRADLYSMGVMLYELTTGLLPFTADDPLAVISQ
                     HLYAPLVPPRAHNPQIPEALDALIVQLLSKQPDDRPRNAGEVWQLLELLETAQQLGMK
                     KPAGQEYTLLDRIVRGRLIGREREVAQMSAAWQQSTSGQGGVLLISGEPGIGKTRLAR
                     ELVAQALI"
     gene            complement(1036..2310)
                     /locus_tag="AC16CB_08870"
     CDS             complement(1036..2310)
                     /locus_tag="AC16CB_08870"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR3342619.1"
                     /translation="MHLESQESPGSHLSLLYQLTQTFNSSLNLDEVLNRVMDEVIAAT
                     HAERGFVMLLEADEKLTFRIARGMDQTTIDAPQFQVSRSVVERVAQKGQAVLTSDAQT
                     DERFNIRQSVVLLGLRSILCVPLKLKGRVIGVIYVDNRLQAGIFTEADLRLLSAIASS
                     AAVAIENARLYQVAVEKGRLEQELSMARRVQVSLLPRKMPQLSGWDFITRWKPARQVG
                     GDYYDFIETDDGLLGLVIADVTDKGMPAALFMAFTRSIIRGNLDHATSPAAGINRVNR
                     LICQESDNGLFVTLFYGLLDPNTGQLTYVNAGHNPPILISSSELGHSTPMLYLESTGI
                     PLGIEEETSYTQNVVQLKPGDFITLYTDGITEAIDASEQEFGLERLQHVLLDHQDQSA
                     SEIAVALEEAIDHYSGDLTSHDDITFIIAKRL"
     gene            2607..>3041
                     /locus_tag="AC16CB_08875"
     CDS             2607..>3041
                     /locus_tag="AC16CB_08875"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR3342620.1"
                     /translation="MSYQTLVSAEELMSHLGDPNWVIVDCRFTLTDTERGRQDYRQAH
                     IPGAVYAHLDEDLSTPHIPGSTGRHPLPAVDSLVATFSNWGISQGMQVVAYDDDPSAS
                     GAIAARLWWCLRWLGHDTVAVLDGGWLRWQKLGYPTQGGEERR"
BASE COUNT          706 a          749 c          825 g          761 t
ORIGIN      
        1 agatcagggc ttgagccacc agctcgcgtg ccaggcgggt tttgccaata ccaggctctc
       61 cgctgataag caatacacct ccctgcccag atgtagattg ctgccaggca gcactcattt
      121 gagcgacttc tcgctctctc cctatcaagc gcccgcgcac aatccgatct agtagagtgt
      181 actcttggcc ggcaggtttt ttcatgccga gctgttgggc agtctctagc agttccaata
      241 gctgccatac ttcaccagcg tttcgtggtc ggtcgtcagg ttgtttgctc aagagctgca
      301 caatgagggc gtccagcgct tctggaattt ggggattatg cgcgcggggg gggacaagag
      361 gtgcgtacaa atgttgagat attacagcca gcggatcatc tgcagtaaag ggcagcaatc
      421 cggtagtaag ctcgtataac attactccca ttgaatagag atctgcgcga ccatcatagg
      481 attgacgaag agccgcctca ggggccaggt aatacactgt gcccacaatc atcccatcta
      541 gggatatatg tgaggcgaca gagcgcgcca gtccaaagtc agtcagctta gcggtcccat
      601 caggggttac cagcacgttt tctggcttga gatcgcggtg gacaatcccg tgagtgtggg
      661 cgtgatccag cgcggcacat atctggcagg caatggatat gatctcatcg atcgactttg
      721 gtttatgctc gtgcaatgat cttcccttaa ccaactccat gacgataaag ctaaccccct
      781 ggtcttcacc ggcgtcatag atatcgacaa tattggggtg gtttaaacca gcagctgcct
      841 gtgcttcatg taataaccgc gagcgaccct cgctacccaa gcgaatattt ctcgcctcgc
      901 tgagaacttt gattgcgacc tggcggttca ggaggttatc gtgcccctca taaaccatac
      961 ccatgccacc tttgccaagt tcttgcataa gctgatagcg acccataagt tggctaagtg
     1021 tcgtcatcgt tttttctata agcgtttagc tatgatgaat gtgatatcgt catgggatgt
     1081 caaatcgcca gagtagtgat caatagcttc ttctagggct acagcgatct cggaggcaga
     1141 ttgatcctgg tgatccaaga gcacgtgttg caatcgttcc aatccaaact cttgctcact
     1201 cgcatcaatc gcttcggtaa tcccatcggt atataaggtt ataaaatccc ctggtttgag
     1261 ttgaacaacg ttttgtgtat atgaagtttc ttcttcaatc ccaagcggta tgccagtgct
     1321 ttcaagatac agcattggag tggagtgtcc caattccgag gatgatatca ggatgggagg
     1381 gttgtgtcca gcgttcacgt atgtgagctg cccagtattt ggatcgagca gtccgtaaaa
     1441 cagagtgaca aaaagcccgt tatcagactc ctggcaaatc aggcggttaa cacggttaat
     1501 tccagcagca ggactggtgg catggtctag gttgccacgg atgatgctcc gagtgaaggc
     1561 catgaacagc gcggcaggca tacctttatc agtcacatcg gctatcacta ggcctaacag
     1621 tccatcatcg gtctcaataa agtcgtaata gtcaccacct acctgacgag cgggtttcca
     1681 gcgagtaata aaatcccagc cggatagctg aggcatcttg cgaggaagca agctgacctg
     1741 aacacggcgt gccatgctta attcctgctc caagcgccct ttctcgactg ccacttggta
     1801 gaggcgagcg ttctcaatgg ctactgctgc actggaggcg attgcgctga gcagccgcag
     1861 gtcggcttca gtgaagatgc ccgcttggag acgattatct acgtagataa cgccgatcac
     1921 gcggcctttt agtttcaaag ggacacacaa gatcgagcgc agacccagga gcacgacgct
     1981 ctgccggatg ttaaaacgtt cgtccgtttg ggcatcgctg gtcagcaccg cttgtccttt
     2041 ttgggcaaca cgttccacta cgctgcggga tacctggaac tggggtgcgt caatagtcgt
     2101 ttgatccatc ccacgcgcaa ttcgaaaagt gagtttctca tcggcctcaa gcagcataac
     2161 aaaacctctc tccgcatggg tggcggcaat gacctcgtcc atcacccggt ttaacacttc
     2221 gtccaggttg agcgaggaat tgaaggtttg cgtaagctga tagagcagac tgagatggct
     2281 gcccggtgat tcttgagatt cgaggtgcat tttcctgatc tattgaatat attccgaggt
     2341 atatttgggg tattataccc ctttatgaat ggaatcgtat ccggcatggt ttggcttcag
     2401 ggcaattgca tatgcgctga ggggcatgcg caaccccgca aagagagtgt ttggggtgtt
     2461 ttggtggggg tgacatctat tcgcgggttt gctagttggg tgcacaggtg aggcgtactt
     2521 tgttatagaa ttactataat gggaatacgg gtacagattt caagccattt gacaaggatt
     2581 aggtcatttc gaaacaggat tttcagatga gttatcaaac actagtttca gctgaagagt
     2641 tgatgtcgca tttaggcgat ccgaattggg taattgtcga ctgccgcttc acgctgaccg
     2701 ataccgagcg aggccggcag gattaccggc aggctcatat ccctggggcg gtgtatgccc
     2761 atctggatga ggatttaagc acgccgcaca tccccggctc aacagggcgg catccattgc
     2821 cagcagtcga ttcgctggtt gccacatttt ccaactgggg aattagccaa gggatgcaag
     2881 tagtagctta cgacgatgac ccatctgcca gcggggccat tgctgcccga ttatggtggt
     2941 gcttgcgctg gctaggtcat gataccgtcg ctgtgctgga tggcggttgg ctgcgctggc
     3001 agaaattggg ctatccgacg cagggtgggg aggaaaggcg c
//
LOCUS       DBNGSX010000292         5480 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_301076, whole genome shotgun sequence.
ACCESSION   DBNGSX010000292 DBNGSX010000000
VERSION     DBNGSX010000292.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5480)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_301076"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            415..1020
                     /locus_tag="AC16CB_08880"
     CDS             415..1020
                     /locus_tag="AC16CB_08880"
                     /inference="COORDINATES: protein motif:HMM:NF022747.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HIR3342621.1"
                     /translation="MNKAKSKASKRQLIRERQRKQQIISRYIWVGVGIVVLAIIGILV
                     WQTARPAVGEVVPIPISSSEHVPEDTQPGPYPSDPPAGGVHFAGTLPAKLYLEEDLAT
                     LPQYPEGYLVHNLEHGYVIFWYNCDVVGEAGCEQLITQLQEVIDSFNGFKVMAFPWKS
                     LDIPVAATSWGRLQRFESFDPGLARRFVRNNRNKAPEPNAP"
     gene            1285..2061
                     /locus_tag="AC16CB_08885"
     CDS             1285..2061
                     /locus_tag="AC16CB_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342622.1"
                     /translation="MRNLSIALLSLTIFIAAGCAGQAAVLYAPNPSETPAPTATLTFT
                     PSPEPTATPLPTHTPTPTTTPTVTASFTPSPSPTPTSEFPIAITTEERAFCRYGPGKA
                     YLFSHELNKGDMAEVHGRNASKTWLWLKPYNLDRHCWAAASVLEVNGDIASVNVVTSR
                     LPKSTLYESPVWVDAERDGDEVTVEWDEVWMTEDDFRGYLIEATVCQNGYLIFVPVQT
                     DDTHFTFTDETGCSGSSGGVLYTVEKHGYTDPVEIPWPEN"
     gene            complement(2085..2528)
                     /locus_tag="AC16CB_08890"
     CDS             complement(2085..2528)
                     /locus_tag="AC16CB_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3342623.1"
                     /translation="MRTVKSLLDEKGNEIWSTTPDAKVIEALQILAEKDVGALLVLDG
                     EKLVGIFSERDYARKVILKGRSSKEIPVRDIMTYHVIYGHPSQTVEQCLELMTEKHVR
                     HLPILEDDQLIGIVSIGDLVKAIIAEQKKLIARLERYILQRTSLV"
     gene            complement(2553..3014)
                     /locus_tag="AC16CB_08895"
     CDS             complement(2553..3014)
                     /locus_tag="AC16CB_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR3342624.1"
                     /translation="MISPEQLRRYPFFGRLSETQLIEIAMISDHVSFEAGEIIAKEGD
                     LAEALYFLEEGAVDLYYIPETGSILQEGYPVGTINPGEPFSISAVIEPHILTSTVRAS
                     GPCRAIRIQAQALRRLMKNDRQLAYVITHHAAKAVIERLHATRTQLAAARA"
     gene            complement(3120..3593)
                     /locus_tag="AC16CB_08900"
     CDS             complement(3120..3593)
                     /locus_tag="AC16CB_08900"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3342625.1"
                     /translation="MISAKLLRDHSFFAIFNEAQLKAISEIVTYEDYQQGAEIFEEGM
                     TAEALYVLLTGRVELSYTIIPSYHPERRKESVVGEINPGEVFGISALIEPHILTMTAR
                     ATQSSRVIRFEAGELRRLMKGDQEMAYVVIDQLAKSAIQRLNATRRQLATAWEPL"
     gene            complement(3590..5086)
                     /locus_tag="AC16CB_08905"
     CDS             complement(3590..5086)
                     /locus_tag="AC16CB_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HIR3342626.1"
                     /translation="MKRISIDPITRLEGHGKIEIFLDEDSEVANAYFQIPEFRGFEQF
                     CVGRPAEEMPRITSRICGVCPEAHHMASTKALDALFHIEPSPAVEKLREMFYSAFYVT
                     DHTTHFYALGGPDFVLGPDAPPAERNILGVIHKVGVEIGKEVIACRQRNHHVIQMVGG
                     RGVHPVAGLPGGWSKSINEEERHEIEEIARQNVVFALFSLKIFEDIVLKNQSYLDLIL
                     SDIFTHRTYSMGTVDANNHVNFYDGLIRVVDPQGREFAKYPAKDYTKHLAEHVEPWTY
                     LKFPYLKQVGWKGFVDGEESGVYCATPLSRLNAADGMATPKAQEAFEKFYETLGSEKI
                     DGRYQPVHYRLATHWARLIELLYAAERMLELIIDPEITEPHTRRQVTEVPDEGVGCVE
                     APRGTLTHHYWTDENGILTQVNLIVGTTNNYAPIAMSVKKAAQALIHKGVDISEGLLN
                     RIEMAFRVYDPCFACATHSLPGQMPLDVTIRRLDGSILTTISREEGTS"
     gene            complement(5098..>5480)
                     /locus_tag="AC16CB_08910"
     CDS             complement(5098..>5480)
                     /locus_tag="AC16CB_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HIR3342627.1"
                     /translation="EKHEKQITRFVRPYDIIPEPGLCLLEQELVCLGPATRSGCGALC
                     PQVGMGCRGCYGALDNVSDQGARMLSAIASVSSAGAVGEEEAVLAEKAQELMGTLVDP
                     AGTFYRFSLAKSLLVRSRQATHSE"
BASE COUNT         1292 a         1519 c         1343 g         1326 t
ORIGIN      
        1 cgattttggc ggccgcgtgg gccttactgc ttggcgttgt gttgcctgcc atcgatggcc
       61 tgcttgtgcg cacgcctgac gcgcaaggcc tgccataaca agcctgcagc tgacattagg
      121 cttgacccga cccaaccatg agcgatcaag gaggcaagcc tgaggggtat tgcggatcgt
      181 cgatgtaggc gagcaggcct gcctcccaag cgctcaagat gccagcatat ttttgattcc
      241 acaattaaat aacaaagaca aaccgctttt tagggtaaaa ttggtttaaa ttaatccgtc
      301 aaccatgcat ggatttacca ccttataaaa agcagaagtt acattggtgg ggaagttatg
      361 gggtacttcc ccaccagttt ccatcatctg cgcatgttct aaagaggtta tttaatgaac
      421 aaggcaaaga gtaaggccag caagcgacag ctcatcaggg agcgccagcg caaacaacag
      481 ataatctcca gatatatctg ggtaggggtg ggtattgttg tattggcaat cattggcatc
      541 ctggtctggc agactgccag accagccgta ggcgaggtcg tgccgatccc catatcgtcc
      601 agcgagcacg tcccggagga tacgcagccc ggcccgtacc cgtctgatcc accagccggc
      661 ggtgtgcatt ttgcaggaac actgcctgcc aaactttacc tagaagaaga cctggctacc
      721 ctcccgcaat acccagaggg ctatctggtt cacaacctag agcacggcta tgtgatcttc
      781 tggtacaact gcgacgtggt tggtgaagcc ggatgtgagc agctcatcac ccagcttcag
      841 gaagtgatcg actctttcaa tggcttcaaa gtgatggcct ttccctggaa gtctctggat
      901 attccggtgg cagcaacctc ctgggggcgg ctgcaacgct tcgaatcctt tgatcccggc
      961 ctggcccgcc gcttcgtgcg taacaaccgc aacaaagcgc ccgaaccaaa cgccccttga
     1021 tcgagcattc ttgatttagg caccactccc attcagcgcg gctattaaat ttctgatggc
     1081 atggatggcc tcagatcgcg cctgaaatga tttgctcaca ggtccctcag acccaggatc
     1141 gaagtacatt gtccatgttc ccgagctcgt tgttgaccaa tcagatagcc atgcttaccc
     1201 aatgatgggg atatatactc caccgactta agtagaactt aatacaaaga gcttattttt
     1261 gttcagtcca gttacaatag tgcgatgcgt aatttatcaa ttgccttgct atccttaact
     1321 atctttattg ccgcgggatg cgcaggacag gctgctgtgc tctatgcccc caatccaagc
     1381 gagactcctg cacccacagc gactctcaca ttcaccccca gtcccgagcc gaccgctacc
     1441 ccacttccca cccatacccc cacgcctacc accactccca ccgtcaccgc ctcgttcacc
     1501 ccatcgccct cgcctacgcc gacgtctgag ttcccgattg cgatcacaac agaggaacgc
     1561 gccttctgcc gctatggccc tggaaaggca tacctgtttt cgcatgagct caacaaaggc
     1621 gatatggcag aggtacacgg gcgcaatgct tctaagacct ggctgtggct caagccatac
     1681 aacctagata gacactgctg ggcagcagcc tcggtgttgg aagtgaacgg cgacatcgct
     1741 tcggtgaatg tagtcacctc gcgcttgcca aaatccacgc tctatgagtc tcccgtctgg
     1801 gtcgatgcag agcgtgatgg agacgaggtg actgtcgagt gggatgaggt atggatgacg
     1861 gaggacgatt tccgcggtta cctcatcgaa gctaccgtgt gccagaatgg ttatctgatc
     1921 ttcgtccccg tgcaaaccga tgacacccat ttcaccttca ccgatgaaac ggggtgctcc
     1981 ggctcatctg gtggagtgct ctacactgtg gaaaagcatg gctataccga cccggtggaa
     2041 atcccctggc cagaaaatta aatatagcca ccccttaccg atctttatac caggctggtt
     2101 cgctggagga tatacctttc caatcgagcg atcaattttt tctgctcagc aatgatcgcc
     2161 ttgacaagat ctccgatcga gacgatgcca attaactggt cgtcttctag gataggtaaa
     2221 tggcgaacgt gcttttccgt catcaattcc aggcattgct cgacggtctg gcttggatgg
     2281 ccataaataa catgataggt cataatatct ctgaccggga tttctttgga cgacctgccc
     2341 ttcaggatca ccttgcgagc ataatccctc tcagagaaaa tacccacgag cttctctcca
     2401 tccagcacca gcaaggcccc aacgtctttt tccgccaaaa tttggagagc ctctataacc
     2461 ttggcatctg gggtggtaga ccaaatctcg ttgccttttt catctagcaa gcttttgacc
     2521 gttctcatcg cgtatcctaa ctctataaaa ggctaagccc gagcggcagc aagctgtgta
     2581 cgagtggcgt gcaaacgctc gatgactgct tttgctgcat ggtgggttat cacatatgcc
     2641 aattgccggt catttttcat caggcggcgc agggcttgcg cctggattct gatcgcccga
     2701 cacggtccag aagcgcggac cgtcgaggtt agaatatgag gctcgatgac tgccgagatc
     2761 gagaaaggct cgccggggtt gattgtgcct acgggatatc cttcctgaag gattgatccc
     2821 gtttcgggta tgtaatacaa atcaacagcg ccttcctcca ggaagtatag cgcctcggcg
     2881 aggtctcctt ctttggctat gatttcaccc gcctcgaagg atacatggtc ggaaatcatg
     2941 gcgatctcaa tcaattgagt ttcgcttagc cggccaaaga atggatagcg gcgtaactgt
     3001 tctggagata tcatcgctgg cctcctccta atttagtgaa aagaattttt tgcgtgacgc
     3061 atacagtgcg atctgctttt gcaataggca agacctacaa tcgctagatt tttcggattt
     3121 tacaacggct cccacgctgt agccagctgc ctgcgtgtgg catttaaacg ctgtatggct
     3181 gattttgcca actggtcaat caccacatat gccatctctt gatctccctt catcaatcgg
     3241 cgtagttccc cggcttcgaa ccggatcaca cgactggatt gggttgctcg cgcggtcatg
     3301 gtgagtatgt gaggctcaat cagtgcagaa atgccgaaga cttctccagg attgatctcg
     3361 ccgacaacac tctctttacg cctttcagga tgataagacg gaataatcgt gtaggacagc
     3421 tcgacgcgac ctgttagcaa aacatacaag gcctcagctg tcatgccctc ctcaaagatt
     3481 tcagcacctt gctgataatc ttcatatgtc acaatttcag agatagcctt gagctgagct
     3541 tcattaaata tggcaaaaaa tgaatgatcg cgcaataact tggcagatat catgacgttc
     3601 cctcctctcg agatatcgta gtcaggatac ttccatccaa cctgcgaatg gtgacatcta
     3661 aaggcatttg acctggcaac gagtgtgttg cgcaggcaaa acaggggtca taaactcgaa
     3721 aagccatttc aatccggttg agtaagcctt cgcttatatc caccccttta tggataagcg
     3781 cctgggcagc ctttttaacc gacatggcga tgggagcata gttgttggta gtgcccacga
     3841 tcaggttgac ttgggtgagg atgccattct catccgtcca gtaatggtga gttagggtgc
     3901 cgcgcggcgc ctcaacgcag cctacgccct catccgggac ttcagtgact tggcggcgcg
     3961 tatgaggctc ggtgatctct gggtcaatga tcagctccaa catgcgttcc gcagcataga
     4021 gtagctcgat caagcgagcc cagtgtgtgg ctaggcggta gtgcacaggc tggtagcgcc
     4081 catcgatctt ctcggatcca agagtttcgt aaaatttttc aaaagcttcc tgagctttgg
     4141 gagtagccat accatcggca gcgtttaacc gcgatagcgg agtggcgcaa tacaccccac
     4201 tctcctcccc atccacaaat cctttccacc ccacctgctt caagtaaggg aactttaagt
     4261 atgtccaggg ctcgacatgc tcggccaggt gtttggtata gtccttagct ggatatttgg
     4321 caaattctct gccctgggga tcaaccacgc ggatcaatcc atcgtagaaa ttgacatggt
     4381 tgttcgcatc taccgtgccc atcgaatagg tgcggtgggt aaaaatatca gaaaggatca
     4441 agtcgagata agactggttc tttaacacaa tatcttcaaa tatcttcaag ctgaacaggg
     4501 caaagaccac attctgtcgg gctatttcct caatctcatg acgctcttcc tcatttattg
     4561 atttgctcca accgccgggc agccctgcga cgggatgaac cccacgccct cctaccatct
     4621 gtatgacgtg gtggtttcgc tggcggcatg cgatcacttc cttcccgatt tccacaccaa
     4681 ctttatggat cacgcccagg atattgcgct cggcgggcgg tgcatctggt cccaacacga
     4741 aatccgggcc tcccaaagca tagaagtgtg tggtgtggtc ggttacatag aacgccgagt
     4801 agaacatctc acgcagcttt tcgaccgccg gcgatggctc aatgtgaaat agtgcgtcca
     4861 gcgctttggt ggaggccata tggtgagctt ccgggcagac accacagatt cgtgacgtga
     4921 tgcgcggcat ctcctccgcc gggcggccca cacagaattg ctcaaagccg cgaaactcag
     4981 gaatctggaa gtatgcatta gcaacttcgg aatcttcatc gaggaagatt tcgatcttgc
     5041 catgaccttc cagccgcgta ataggatcaa tggagatgcg tttcatagca gccctcctta
     5101 ttcactatgg gttgcctgcc gtgaacgcac aagcaacgac tttgccaggc taaaacgata
     5161 aaatgtgcct gctgggtcta ccagtgtacc catcagctct tgagcttttt ccgccagcac
     5221 ggcctcctct tcacccaccg ctcccgcgct gctgaccgaa gcgatcgcgg acaacatcct
     5281 ggctccctgg tctgacacat tgtccaaagc tccgtagcaa ccgcgacagc ccatccctac
     5341 ctgcggacag agggctccgc agcctgagcg agtggctgga cccaaacaga ccagttcctg
     5401 ttctaacaag cacaaccctg gctcagggat aatgtcgtac gggcgaacaa aacgagttat
     5461 ttgcttctcg tgcttctcca
//
LOCUS       DBNGSX010000293         2558 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_370633, whole genome shotgun sequence.
ACCESSION   DBNGSX010000293 DBNGSX010000000
VERSION     DBNGSX010000293.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_370633"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1163
                     /locus_tag="AC16CB_08915"
     CDS             <1..1163
                     /locus_tag="AC16CB_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342628.1"
                     /translation="RTVVIWDQDFSAGALVETELAFFAQDNDGNVWRMGEYPEVYEEG
                     KLVEAPAWISGLKGAVAGIMMRADPQLGMPSHSQGWAPSVDFTDRGQVDQVGQETCVP
                     AGCYEDVLVVAEFSEAEPDAFQLKYYAEDLGNIRVGWRGADATKETLELVEIIQLSPE
                     ELVEIRAQALELEERAYEISEEVYDQTPPIEIPQDSASVPEPTQGTVTDGATAEVVVY
                     ASDLPESALSEFEVWDEPVSPGGKMLGVTNNGDEMDPPPEDDPHVTFNVQVQDSIPYR
                     CWIHMKVGAPKGKSQANVFFVQFSDAMKQSNEDAFAPETDSYLTAQGPTQEGWTWVGC
                     DLDGSDSLIYFKTSGEITVRLQAGMEGVGFDQFLLSSARFLDEPPSDAVVEK"
     gene            1176..1406
                     /locus_tag="AC16CB_08920"
     CDS             1176..1406
                     /locus_tag="AC16CB_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342629.1"
                     /translation="MENTKSWTSLVAPSGWTDVFIRAVKVFLVAFVMLHLKEFLDGGG
                     FDTPDIAVDALVVAAGTLVLNAILALTTRTRG"
     gene            complement(1538..2275)
                     /locus_tag="AC16CB_08925"
     CDS             complement(1538..2275)
                     /locus_tag="AC16CB_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342630.1"
                     /translation="MKGVIHETVTAATLIIVFLTGCATSQPPISTPTASTSIAEADWL
                     ENFDVSTCNMATTGSNQYFILEPGFQLVLEGGTEVLVITVLDEVVEVDGIQTRVVEER
                     ESRGGELIEVSKNFFAICEQTKDVFYFGEEVDMYQGGQVANHSGAWRSGEGDARFGLI
                     MPGAPSLGMMYYQEVAPGIAMDRAEIISLDETFSTPAGKFSKSLNIKEGTALNPLEVE
                     YKTYAPGIGLIQDQSLLLTEYGFIEGK"
     gene            complement(2308..>2558)
                     /locus_tag="AC16CB_08930"
     CDS             complement(2308..>2558)
                     /locus_tag="AC16CB_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006540032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HIR3342631.1"
                     /translation="GGMISFVPNGGAQAAHRMAESTRIFALAESLGGVESLIEVPAAM
                     THASTAASPLAVDPGLIRLSVGLEHVDDLIEDLDSALK"
BASE COUNT          617 a          642 c          705 g          594 t
ORIGIN      
        1 tgcgtactgt agttatttgg gatcaggact ttagcgcggg tgcgttggtg gagacggagc
       61 tggccttctt tgcgcaggac aatgatggca atgtctggcg catgggtgag tatccagagg
      121 tatacgaaga aggaaagctc gttgaggctc cggcttggat ttcaggcctc aaaggtgcgg
      181 tggcagggat aatgatgagg gcggacccgc agttagggat gcccagtcac tcccagggat
      241 gggcaccctc agtggatttc actgaccgcg ggcaggtaga tcaggtaggt caggagacct
      301 gtgtcccagc gggttgttat gaagatgtgc tggtggttgc agagtttagc gaggcggagc
      361 ccgatgcatt ccagctcaag tactacgctg aggatttggg caatatacgt gttggctgga
      421 ggggtgcgga tgcaaccaag gagacactag aactggttga gatcatccaa ctcagcccgg
      481 aagagctagt agagattcgc gcacaagcac tggaattgga ggagcgcgcc tacgagatca
      541 gcgaagaggt atacgatcaa actccgccaa ttgaaattcc ccaggattcg gcctcagttc
      601 ctgagcccac ccagggtaca gtgacggacg gtgccacggc ggaggttgtg gtctacgcct
      661 cagacctacc agaaagcgcc ttatccgaat ttgaagtttg ggacgagcca gtatcaccag
      721 gaggtaagat gctcggagtt accaataacg gcgatgagat ggaccctcct cccgaggatg
      781 acccccatgt gactttcaat gtgcaggttc aagatagcat cccttaccgt tgctggatac
      841 atatgaaagt tggcgcaccg aagggcaaat cgcaagcgaa cgtgtttttc gtgcaattct
      901 ctgatgccat gaaacaatca aatgaagatg ccttcgctcc ggaaacggac agctacctga
      961 ctgcgcaagg cccgacccag gaaggctgga cttgggttgg ctgtgattta gacgggagcg
     1021 attccctgat atacttcaag accagcggcg agatcaccgt acgtttgcag gccgggatgg
     1081 aaggcgttgg gttcgaccag tttttgttga gctccgcacg tttcctggac gagccgccat
     1141 ccgatgcagt cgtagaaaaa tagagggtga agaatatgga aaataccaag agctggacta
     1201 gcctggttgc tccttctgga tggacagatg ttttcatccg agccgtcaag gtgtttttgg
     1261 ttgcatttgt gatgttgcac ctgaaagagt ttctcgacgg tggtggtttc gacaccccag
     1321 atatcgcggt tgatgcgtta gtggtcgcag ctgggaccct cgtattgaat gcgatcctcg
     1381 cgctgactac acgtaccagg ggttagctta cctgccatgt aggtcagacc aagcaagccc
     1441 gaaaaaactg cgagtttctc gactgttacg aggattatag gtacaacagg tcgaggccgg
     1501 tgcggacaat acacgcaccg gcttgtcaac atgtatatta ttttccctcg atgaacccat
     1561 actcagttaa tagtagactt tgatcttgaa tgagcccgat tccaggggca taggttttat
     1621 attccacctc taagggattt aatgctgtac cctcttttat attcagggat tttgagaact
     1681 tacctgcagg tgtgctgaac gtctcgtcca ggctaatgat ttcggcacgg tccatagcaa
     1741 tacccggtgc gacctcctga taatacatca tgcccaacga cggtgcgcca ggcatgatca
     1801 aaccgaacct cgcatctcct tctcctgatc gccatgctcc gctatggttg gccacctgac
     1861 ctccctggta catatcgacc tcctcaccaa agtaaaagac atctttggtc tgctcacaaa
     1921 tagcgaagaa atttttggac acttcgataa gttcgccgcc ccgcgattcg cgctcctcca
     1981 ccacgcgggt ctgaattcca tctacttcga cgacttcatc gagcaccgtg atgacaagca
     2041 cctccgtccc tccttccaga acgagctgaa agcccggctc caggatgaag tactggttgc
     2101 tacctgtcgt agccatgttg catgtcgaaa catcgaaatt ctccagccaa tctgcctcag
     2161 caattgatgt cgaggcagtc ggtgtgctta tgggaggctg agatgtcgca catccggtca
     2221 gaaaaactat gattagagta gcagcagtga cggtttcatg aatcacgcct ttcatattct
     2281 attcctttgg aaataaaggt atctggacta tttcaacgcg ctatccaaat cttcgattaa
     2341 atcatccaca tgctccagac ccacggaaag gcggatcaac ccagggtcga ccgccagggg
     2401 cgaggcagca gtagaggcgt gggtcatggc agccggcact tcgatcagcg attcgactcc
     2461 tccaagagat tctgccaggg caaagatgcg cgtggattcg gccatacgat gggcagcctg
     2521 agcgccaccg ttgggcacga aagagatcat gccacctc
//
LOCUS       DBNGSX010000294         3990 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_786576, whole genome shotgun sequence.
ACCESSION   DBNGSX010000294 DBNGSX010000000
VERSION     DBNGSX010000294.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_786576"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="AC16CB_08935"
     CDS             complement(<1..777)
                     /locus_tag="AC16CB_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR3342632.1"
                     /translation="MSVGQIVPGQRAMWKLAEKLGEGDAGEVFLIESTLQGKQAILKR
                     PRKGAYSSDILRQAAQIRSEGSILRALSEVSFSSQVVHLSTPAVLDESRPEDGGGERY
                     FIIIERASGIDLKSLRQISHFGVLESIQSLAGEDNRFFIEKIARFGQIPEPILIRILG
                     GVIHLLETIHSSQVSMDSTAHAGLIWNDVKPEHLFWDPHQTRLTVIDWGNGQFLEADG
                     ATKDRRYTRNDDYSQFIQEIGGFLAESNPSLHKRLEWPKSI"
     gene            1332..2369
                     /locus_tag="AC16CB_08940"
     CDS             1332..2369
                     /locus_tag="AC16CB_08940"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017688840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HIR3342633.1"
                     /translation="MFAVGQKQINISQEQSWHETIAKYQTPDARRSWWQVANTIIPYF
                     FLGFLMYLSLSVSYWLTLLLAIPAAGFMMRTFIIFHDCGHGSFFKSKRANDTMGIFTG
                     ILTFTPYYRWRHDHAVHHACAGDLDRRGVGDVWMLTVDEYQALPTWRKLVYRVVRHPL
                     VMFTVGSLAVFLIGHRFASRASGKRERYSVYWTNLALLGIALLMSALIGFKAYVLIQL
                     PILAIGTSIGVWLFYVQHQFDGVYWERHEGWKFVDAALWGSSYYKLPRILQWFTGNIG
                     FHHIHHLGPRVPNYNLEKCHYENPLFQQVKPLTLRSSMRSLFYRLWDEEQHKMVGFAS
                     LKKASALPGGV"
     gene            complement(2440..3867)
                     /locus_tag="AC16CB_08945"
     CDS             complement(2440..3867)
                     /locus_tag="AC16CB_08945"
                     /inference="COORDINATES: protein motif:HMM:NF018012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR3342634.1"
                     /translation="MNTLPHVQPRLSLLSPEQIEVVHTKSLDILARVGLRVDSGKAAE
                     ILAKADGVKFITPDHAVIQPKLVEWAIQSAPSTIQVYNRRGEPAFQLGADRTRFGVGV
                     TNLYYQDPLSDQISPFNRELLARSVRLGDALPAYDLVSTPGILRDVNPGQADLFAILE
                     MAANTQKPLVILVSDEKQFAPGLDLLQEFHGDLAEKPFVIPYFNPVSPLILNLETAHK
                     MITSIQRGLPFIYSNYGMVGMSTPITPAGSLALLNAELLAGLVFSQIVRPGAPVILGS
                     LPAYFDMKTMVDFYDPQTMLLNLACAEMMAHYHLPHAGTSGSGNGWGADLLAAGALWI
                     NHLTACLGVVGLAPFVGGALTSKVFSPTSVVYSNEIIEQALLFSQGFKLDEDAFAMDD
                     IEAAGPGGHFLMTDRTYNMFRSAYHMSSIFPRWSLEKWEDEGQPKADSYLRQRTVQII
                     EDAKPPEDHVEWIREGEKRIKRMTS"
BASE COUNT          865 a         1047 c         1091 g          987 t
ORIGIN      
        1 tatgctcttg ggccattcca ggcgtttatg caggcttggg ttcgattcag cgagaaaacc
       61 ccctatctct tggatgaact gcgaatagtc atcattccgt gtgtaacggc gatcttttgt
      121 ggctccatct gcctctagaa attgaccatt accccagtca atgaccgtca accgcgtttg
      181 gtggggatcc caaaatagat gttccggctt gacgtcgttc catatcagcc ccgcgtgcgc
      241 tgtggagtcc atcgaaactt ggctggagtg aatcgtttcc agcaggtgaa ttaccccccc
      301 taaaattcgg atcaagatag gctctggaat ctggccgaat ctggcaattt tttcgataaa
      361 gaacctgttg tcttctcccg caagggattg gatactctcg agaacgccaa aatggctgat
      421 ctgtctcaat gatttgaggt cgattccaga ggcccgctca ataatgatga aataccgctc
      481 gccgccccca tcttctggcc ggctctcgtc caacacggca ggcgtggaga ggtgcaccac
      541 ctgactggaa aaagaaacct cgcttaatgc tctgagaatg cttccctcgc tcctgatctg
      601 ggcagcctgg cgtaagatat cgctggagta tgcgcccttg cgaggtcgtt tcaagatggc
      661 ttgtttgcct tgtagagtcg actcgattag aaatacctcg cccgcatccc cctcccccaa
      721 tttttccgcc aatttccaca tagcccgttg accgggaacg atttgaccga cactcatact
      781 gttattttct gtttcaccta ctggtttatc cgagactcac gtattttaga agttcgcagt
      841 ttggcagata atgggtgagc tctactcccc caactctttc ccttgaactg cgcacgtcct
      901 tctattataa cgagttctgc ggcctgcttt atcgcaggca gtagattata gcgcaaaaaa
      961 gtgtggtcta agcgatgact ggtaacgcga cagggcaatt gcatatgcca tgaggggcat
     1021 gcgcaatccc gtaaaaagag cgtttggggt gttttggtgg gggtatgccc ccaccaaaac
     1081 accccatttt ctctcctcag tggtgggaaa ttgcccaaat gcgattgccc tagtaacgcg
     1141 atcgaactag gctaacgggt gatataattg aaacaactgg ggggttcaga tttattagag
     1201 tagtggaagg cgcctgaacg ccggcattgc tatcgttttt tcaaccctgg tttactccca
     1261 caaagcactg atcgagttga aaagcgagtt taatcgtctg cgctcgagca gatgatggaa
     1321 aaggcgagaa aatgttcgca gtgggtcaaa aacagataaa tatctcacag gagcaatctt
     1381 ggcatgaaac gatcgccaaa tatcaaaccc ctgatgcgcg gcgaagttgg tggcaggtcg
     1441 ccaatactat aatcccctat ttctttttgg gtttcttgat gtatttgagc ttgagtgttt
     1501 cctactggct taccctgctg ttggcgatcc cggcggccgg tttcatgatg cgcactttca
     1561 tcatttttca tgattgtggg cacggctcct tttttaagtc gaagcgcgcc aacgatacga
     1621 tgggaatttt caccggcatc ctaactttca ctccttacta tcgttggcgt catgaccatg
     1681 ccgtgcacca cgcctgtgct ggtgacttgg accggcgcgg tgttggtgac gtgtggatgt
     1741 taaccgtcga tgaatatcaa gctctgccaa cctggaggaa gctggtctac cgtgtggtac
     1801 gccatccttt ggtgatgttc acagtcggct cgttggctgt cttcctaatc gggcaccgct
     1861 ttgccagccg cgcttctggg aagcgtgaac gctatagtgt atattggaca aaccttgccc
     1921 tgttgggcat tgccctcctg atgagcgcgc tcatcggctt taaagcgtat gtgctcatcc
     1981 agctgccgat attagctatc ggaacttcca ttggggtgtg gctcttttat gtccagcacc
     2041 agtttgatgg ggtctattgg gaacgtcatg agggctggaa atttgtcgat gcagcgcttt
     2101 ggggcagctc gtactacaaa ctccccagga tcttacagtg gtttaccgga aatatcggct
     2161 ttcaccacat ccaccatctc ggcccgcgcg tccccaacta taacctagaa aaatgccatt
     2221 acgagaaccc tctcttccag caggttaaac cgctaaccct gcgctccagc atgcgatcct
     2281 tgttttatcg cttgtgggat gaagagcagc acaaaatggt tgggtttgca agcttgaaaa
     2341 aagccagcgc attgccaggt ggggtgtaaa tcgtcccaat caaggcaatt gggctggtat
     2401 ttggttttga aatgacaggg ggagattgat taaacggcgt cagctcgtca ttctcttaat
     2461 acgcttttcc ccttcgcgta tccactccac atgatcctcc gggggcttgg catcttcgat
     2521 aatctggact gtcctctggc gcaaatagga gtcggctttg ggttggcctt cgtcctccca
     2581 cttctctaag ctccagcgag ggaagatgct gctcatgtgg taggcgctgc ggaacatgtt
     2641 gtaagttcga tcggtcatta agaagtgacc gccaggccca gcggcctcaa tatcatccat
     2701 ggcaaaggcg tcctcatcca gcttgaagcc ctggctaaag agcagcgctt gctcaataat
     2761 ctcgttggaa tacactaccg aagtcgggga gaagaccttg gacgtcaggg cgccgcctac
     2821 gaagggtgct agcccgacca cacccaggca ggcggtcagg tggttgatcc acagggcgcc
     2881 agccgcgagc aggtcggctc cccagccgtt cccgctgcca gatgtgccag cgtgggggag
     2941 gtgatagtgt gccatcatct cggcgcaggc caggttgagc agcatggtct gcgggtcgta
     3001 gaagtccacc atggtcttca tgtcgaagta tgccggcagg ctgcccagga tcaccggcgc
     3061 gccggggcgt acaatctggg agaacaccaa cccggcgagc agctctgcgt tgagcagggc
     3121 tagcgatcca gccggcgtga tcggggtgga catgcccacc atgccgtagt tggagtaaat
     3181 gaagggcagc ccacgctgga tagaagtgat catcttgtgg gctgtctcca ggttcaggat
     3241 gagcggagac accgggttga aataggggat gacgaaaggc ttttcagcca gatctccatg
     3301 aaattcctgc aagagatcca gcccgggggc gaactgcttc tcatcggata ccaggatgac
     3361 caggggcttc tgcgtgttag cagccatctc caggatcgcg aacaggtcgg cttgccctgg
     3421 atttacatca cgcaggatgc ccggcgtgga gaccaggtca taggcaggca gggcatcccc
     3481 caagcgcact gagcgtgcca gcagttcgcg gttgaaaggg ctgatctggt cgctgagtgg
     3541 gtcctgatag tacaagttgg tcacccccac gccaaaacgg gtgcggtcag cgcccagctg
     3601 gaacgccggc tctccgcggc gattgtagac ctggatggtg gatggcgcgc tttggatagc
     3661 ccactccacc agcttgggtt ggatcacggc gtggtcgggg gtgatgaatt tgactccatc
     3721 agctttggca agtatctcgg ccgctttccc tgaatcaacg cgcaacccaa ccctcgccag
     3781 aatatccagg gatttggtgt gcaccacctc gatctgctct ggagaaagca agctgagccg
     3841 gggttggacg tgggggagtg tgttcatctt tttccttccc atgggtgttc accgaaatcg
     3901 gttgagctct caggtgtgat agcgtcccgc aggtttccct aaaacgagca tgcgtgataa
     3961 tcaacctgcg ggacgttcat tactctggtg
//
LOCUS       DBNGSX010000295         3962 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_578318, whole genome shotgun sequence.
ACCESSION   DBNGSX010000295 DBNGSX010000000
VERSION     DBNGSX010000295.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_578318"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..149)
                     /locus_tag="AC16CB_08950"
     CDS             complement(<1..149)
                     /locus_tag="AC16CB_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3342635.1"
                     /translation="MNTKRQHAYDAVVVGSGPNGLTAAITLARTGRSVLVLEAKDTIG
                     GGLRT"
     gene            complement(160..927)
                     /locus_tag="AC16CB_08955"
     CDS             complement(160..927)
                     /locus_tag="AC16CB_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342636.1"
                     /translation="MTLHSPNSPKADSRENAWAPPTARMKVGEMPAGPRNINVEGRQP
                     MSPLQGFGQMWQKTFKVRLSGVDKTPAEVMQVWKDNFPKFQPPENRFYPTMSGIKPGE
                     VLLIEAKVPPLPGLPSMLPVSTGVMVLYADDTTFTVMTPEGHPEAGWNTFSVYEEDEV
                     LVAQCQSLCRPNDPLYDFFNRFLGASSQQDKIWIHVLVSLADHFGVKGQAATTKVLVD
                     PKVQWSQVKNIWQNAAIRTIFYLLGAPLRWLRNLARR"
     gene            complement(996..1349)
                     /locus_tag="AC16CB_08960"
     CDS             complement(996..1349)
                     /locus_tag="AC16CB_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3342637.1"
                     /translation="MTKANVSMNVRTASPNTSIVDIVGEINAFAENVLMDAYTQASSA
                     GAHNILLNFSGLEYMNSSGIGLLVTLLIRVNRQKQRLSAFGLSEHYQQIFELTRLNEA
                     IGIYPDEAAALASLG"
     gene            complement(1390..1734)
                     /locus_tag="AC16CB_08965"
     CDS             complement(1390..1734)
                     /locus_tag="AC16CB_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3342638.1"
                     /translation="MAQHLFEARLRQVSGKAIIDLQGEINAFADANLSEAFTQAEDTD
                     YPDILLNFSDVSYINSTGIALIVGLLARARKNNRRLLVCGLSDHFTEIFEITRLTDYM
                     AIYPNELEALAV"
     gene            complement(1715..3388)
                     /locus_tag="AC16CB_08970"
     CDS             complement(1715..3388)
                     /locus_tag="AC16CB_08970"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding SpoIIE family protein phosphatase"
                     /protein_id="HIR3342639.1"
                     /translation="MNSFFNRAWKSLTSRPEPPAGEPVPPVAQAAQPVKALEFDIAPN
                     DPLLAYFLSTPGLVEIDKLQMDSPALQALRLAQVKVSVPLVSQGELIGLLNLGSRRSE
                     QDYSADDRRLLQTLATQAAPALRVAQLARQQQAEARERERLEQEMRVARIIQQTLLPK
                     ELPDLPGWKVAAHWQPARAVSGDFYDFISFPDGKLGLIAGDVTDKGVPAALVMATTRS
                     ILRTAAERFSSPGRILAHTNDTLCPDIPPNMFVTCLCIVLDPISGYLRFANAGHNLPS
                     LCKTDDVYELRATGMPLGLMPGMTYEEKDVTLEPGDSLLIYSDGLVEAHNPQHEMFGF
                     PRLRECLPCSPQGGWQGEGTMIEYLLEKLAEFTGPTWEQEDDVTFVTLERQPSQSASH
                     SAEDQADVKGEAWQILAEFSLPSEPGNEITAMNLVMEVVRDLISPPVRLERLKTAVAE
                     ATMNAMEHGNRYQADLPVELHVLLSPTRLAVRITDHGGANPIPETEAPDLEAKLAGLQ
                     SPRGWGLFLIKNMVDEMNIHVSDAGHGSIHHTVELIISLKGDDHGSAPV"
     gene            complement(3475..>3962)
                     /locus_tag="AC16CB_08975"
     CDS             complement(3475..>3962)
                     /locus_tag="AC16CB_08975"
                     /inference="COORDINATES: protein motif:HMM:NF014860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR3342640.1"
                     /translation="AIGDATGKSILAHVGMQQGLVCADNIMKPSSEALRRMDYDVIPA
                     VVYSIPEVVSVGHIPTDLSGVEIYKVPFSANLRARIEDFEDGFLKIWVKDDCVVAAQA
                     IGHNVSEIMQELANAIALKTPIAHIAEIIHAHPTYSEITRSALDYALGKAVDFYPLQE
                     T"
BASE COUNT          906 a         1101 c         1055 g          900 t
ORIGIN      
        1 ttggtgcgca aaccaccgcc gatggtgtct ttcgcctcta aaaccagcac cgaacgacct
       61 gttcgcgcca aggtaatggc agcagtcaaa ccgtttggcc cagagccaac aactactgca
      121 tcgtaagcat gctgtctttt cgtattcaat agaagtttct tatcttcggg ccaggtttcg
      181 caaccagcgc agcggcgcgc ccagcaggta gaaaatcgtg cggatggcgg cgttttgcca
      241 gatattctta acctgcgacc actgcacctt gggatcgacc aacactttcg tcgttgccgc
      301 ctgtcccttg accccaaaat gatccgccag ggaaaccagc acgtgaatcc agatcttgtc
      361 ttgctggctg gaggcgccca ggaaacggtt gaagaaatcg tataaaggat cattgggacg
      421 gcatagtgac tggcactgcg ccactaaaac ctcatcctcc tcataaacgc tgaaggtgtt
      481 ccaaccggct tcggggtgcc cttcgggcgt catgaccgtg aaggtggtat cgtcggcata
      541 caagaccatc acgcctgtcg atacgggcag catgctgggc aggccgggca aaggcggcac
      601 tttggcttcg atcagcagca cctcgccggg ctttatgccc gacatggtgg gatagaagcg
      661 gttctcaggg ggctgaaact ttggaaagtt gtccttccag acctgcatga cttcagcagg
      721 ggttttatca accccactca agcgcacctt gaaagtcttc tgccacatct gaccgaagcc
      781 ttgcagcgga gacatgggct ggcgcccttc cacgttaata ttcctgggtc ccgcgggcat
      841 ttcgcctact ttcatacggg ctgtcggcgg tgcccaggca ttctcacgcg aatcagcttt
      901 tggggaattt ggggaatgga gtgtcatttg atacctctat ttgtaataca catccagaat
      961 ggcgattctg taactaataa aataggatcg agttcttacc ctaaggacgc caatgcagca
     1021 gcctcatccg ggtaaatgcc gatcgcctcg ttcagacggg ttaactcgaa gatttgctgg
     1081 taatgctcgc tcaacccgaa ggccgacaag cgctgtttct gccggttgac gcggatgagc
     1141 agcgtgacga gcaatccaat tccagagctg ttcatatact ccagcccgct gaaattcagt
     1201 agaatattgt gcgccccagc actgctggcc tgagtgtaag cgtccattaa gacgttctcg
     1261 gcaaaggcgt tgatctctcc aacgatatcc acaatgctgg tattcgggct ggctgtgcgc
     1321 acgttcatgg aaacattagc tttggtcatc tttaaatttc tccttagcaa tactctagtt
     1381 tttttcctct cagactgcca gggcttccag ctcgtttgga tagatcgcca tgtaatcagt
     1441 caaacgggta atctcaaaga tttcggtaaa gtgatcgctc aatccgcaga cgagcaagcg
     1501 gcggttattt ttacgcgccc gggccagcaa accgacgatc agggcaatgc cagtgctatt
     1561 aatatagctc acatcactaa agttgagcaa gatatccggg taatctgtgt cctctgcctg
     1621 cgtaaacgcc tcacttaaat tggcgtcagc gaaggcattg atctcccctt gcaggtcaat
     1681 tatggctttg ccagacactt ggcgcaacct ggcttcaaac aggtgctgag ccatggtcat
     1741 ctcctttcaa gctaattatt agctcaacag tatggtggat gctgccatgt ccagcatcgc
     1801 tgacatggat gttcatttca tccaccatat ttttgatcaa gaaaagcccc cagccacgcg
     1861 gcgattgaag accggccaat ttagcctcca ggtcgggcgc ttcagtctcg ggaataggat
     1921 tggcaccccc atgatcagtg atacgcaccg caaggcgtgt gggcgagagc aaaacgtgga
     1981 gctccaccgg caaatccgcc tggtagcggt tgccatgttc catagcgttc atggtagctt
     2041 cggcgacagc ggttttcagg cgttccaaac gaacgggggg cgagatcaaa tcccgcacta
     2101 cctccataac caggttcatg gccgtgatct cgttccccgg ctcactgggt agagagaatt
     2161 cggccaggat ctgccaggct tcgcctttga catccgcctg atcttccgca gaatgggaag
     2221 cggattggga gggctggcgc tccagggtga cgaaagttac atcatcttcc tgttcccagg
     2281 tcgggccggt gaattcagcc agcttctcca gcaagtattc aatcatcgtg ccctcacctt
     2341 gccagccacc ctgcggcgag cacggcaagc actcacgcaa gcgcggaaag ccaaacatct
     2401 cgtgctgcgg gttgtgtgcc tccaccaatc catcactgta gatcagcagg ctatcgccag
     2461 gctccagcgt aacatccttc tcttcgtagg tcatgcccgg cataagaccc aacggcatgc
     2521 ccgtagctcg cagctcgtac acatcatcgg tcttacatag ggaagggaga ttatgcccgg
     2581 cgttggcaaa gcgcagatac ccactaatgg gatccaggac gatgcataag caggtgacaa
     2641 acatattagg tggtatatcc gggcagagcg tgtcgttagt gtgcgccagg atgcgccctg
     2701 gcgaggagaa acgctcggcg gcggtcctta aaatgctgcg tgtggttgcc atcaccagcg
     2761 cagctggcac acctttatca gtcacatcgc cggcaatcaa gcccagcttg ccatccggaa
     2821 aagaaataaa atcgtagaaa tcaccactaa cagcgcgggc cggctgccaa tgggcagcca
     2881 ccttccagcc gggcagatcg ggcaattcct tgggcaagag agtctgctgg atgatgcgcg
     2941 ccacacgcat ttcctgttct agccgctctc gctcacgcgc ctccgcctgt tgttgccggg
     3001 ctaattgggc cacacgcaaa gctggtgctg cctgagtggc gagggtctgc aaaagacggc
     3061 ggtcgtcggc agagtaatct tgctcactgc gacgggatcc caggtttagc aagccgatca
     3121 gctctccctg gctgaccagc ggcacagaca ccttgacctg agcaagcctg agtgcttgca
     3181 gggcagggga atccatttgt agcttatcga tctccaccag gccaggcgtg ctcaaaaagt
     3241 aggccagcag ggggtcattg ggggcaatat caaattccag cgccttcacc ggctgggccg
     3301 cctgggcaac aggcgggaca ggttctcccg cagggggttc aggcctactg gtaagagatt
     3361 tccaggctct attgaaaaat gagttcattg gtcatattac tccgttttac gcacgtatta
     3421 tatacaacag gcgtcttgag atcgttacgg aagacgttac ggattactgt tattttatgt
     3481 ctcctgcaaa ggataaaaat ccaccgcctt gcctaaggca taatccaggg cagagcgggt
     3541 gatctctgag taagtcgggt gggcatggat gatctctgcg atgtgcgcga tgggcgtctt
     3601 cagagcgatg gcattggcca gctcctgcat gatctctgag acattgtgcc ctatcgcctg
     3661 tgcagctacc acacagtcgt ctttcaccca aatcttcaaa aaaccatcct cgaagtcctc
     3721 gatgcgcgcc ctcaagttag cggagaacgg gactttatag atctctactc ccgacaaatc
     3781 agttgggata tggcccacag agactacctc ggggatggag tacaccacag ccgggatgac
     3841 atcgtaatcc atgcgccgta aggcctcgct ggagggtttc atgatgttgt cggcgcagac
     3901 aaggccctgc tgcatgccca catgcgccaa gatagacttt ccggtggcgt cgccgatcgc
     3961 cc
//
LOCUS       DBNGSX010000296         6403 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_301195, whole genome shotgun sequence.
ACCESSION   DBNGSX010000296 DBNGSX010000000
VERSION     DBNGSX010000296.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6403)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_301195"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..717)
                     /locus_tag="AC16CB_08980"
     CDS             complement(13..717)
                     /locus_tag="AC16CB_08980"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3342641.1"
                     /translation="MSVLKNDVAAFIDLDNTLYTKFLWQALFAHHRRNRFKRLVVARF
                     VAFHFPLWQLAKAGLVPKDLFYRINATNLAWLVGGVSIERAERIWDWIIDHEILPNLR
                     PEMQAVIEDHRNQVHRVILISGSFTPLLDKLAIRLGLECAIATPLVVKDGHYTGKIVP
                     PLNIGQGKVERLQRFLNDRGNEIDLEKSYFYTDAFVDAPVLEMFGNPIAVYPDSHLAA
                     LAAERGWSVIGCAHVN"
     gene            complement(812..2131)
                     /locus_tag="AC16CB_08985"
     CDS             complement(812..2131)
                     /locus_tag="AC16CB_08985"
                     /inference="COORDINATES: protein motif:HMM:NF053377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF8173 domain-containing protein"
                     /protein_id="HIR3342642.1"
                     /translation="MNFKTSIQNKWGRLAILVLIMVGFLTLAFAGSAQAAEVVTGDPD
                     AVVAAGVVIDDDLIISGDKLVIDGTITGNLFATGIEVVLNGSVEGSVFIGGELLTING
                     DIGGSLYSGGCAITLGPQAAIGRSVYAGGFSLIAESGSHVGYDVHAAVFQNRLNGEVG
                     KDVEVATGAFEFNGSAGGNVDVELGETCPYCLEFVPVEAIQPGLRIADDSTVGGEITY
                     KVTARNYESVDASNIEIQPMEVDFEELRNSVLRFIVVQEIRRAIGEFISLLIVGGLLV
                     YFLKDRVRSVMDKISGSALPSFGWGLLVILLFPLVLMVTLALVIAMAFITGLITLGQL
                     SSIVIGFGGFAIASFVVFFVFVISLVTKAVFAYMLGRLVVGRISQDTLASRWGDVWAL
                     AIGVLIYEIVRTIPIIGLFVAMVVILVGAGAIVRSLRDTLRLSAAIA"
     gene            complement(2225..2464)
                     /locus_tag="AC16CB_08990"
     CDS             complement(2225..2464)
                     /locus_tag="AC16CB_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342643.1"
                     /translation="MTFKQKTTQEKQTPFDISACMAMMEKMMGEHMAGCDCAEMMSQI
                     TGQAEVPDEWLKVMSQMMELHCQPLEGADKRAQVL"
     gene            complement(2572..2934)
                     /locus_tag="AC16CB_08995"
     CDS             complement(2572..2934)
                     /locus_tag="AC16CB_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342644.1"
                     /translation="MITEQFFSKIERRSMAESLYWAGVLVWAGLVFGANSLGVLPQFG
                     SGDAWSWVFIGAGLYGTFMNIYHSTLPDPVTTTWDYIWSGFWLLLGLSGLFAIDMFWP
                     LVLVLIGGVTLIKAISNG"
     gene            complement(2937..3179)
                     /locus_tag="AC16CB_09000"
     CDS             complement(2937..3179)
                     /locus_tag="AC16CB_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342645.1"
                     /translation="MPEFYKILIKGHLQDRWEEWLEGLMICRNADGTTTLSGVLSDQA
                     ALHGVLLKIGNMNLKLISVNPIEEYLEDKNTNRKEK"
     gene            complement(3376..5718)
                     /locus_tag="AC16CB_09005"
     CDS             complement(3376..5718)
                     /locus_tag="AC16CB_09005"
                     /inference="COORDINATES: protein motif:HMM:NF037660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3342646.1"
                     /translation="MINEIAESGVEFSLVLDDYHLITSQKINEALNFFLDHLPPNAHL
                     LISTRVDPPLPLSRYRARSQMVEIRLDDLRFTEAEVRSFLNDLLGLDLSTGDILALLT
                     RTEGWITGLQLAALSMQGRVDKHEFITAFSGSHHYIIDYLVDEVMARQIEKIQTFLYQ
                     TSILDRFSASLCNDVLEISNSNDVLQALDRASLFLIPLDDEHTWYRYHHLFADFLRQR
                     LGEREPGSIPKLHRRASKWLEKNKFLTEAINHSLAGEDFEQAARIVESLGPEMMMRSE
                     FDQLSSWLDAIPQQMVVAWPWLCIIRAWMCQRWARLKEAEENLQYAERALEDENTPEP
                     VGGKDVIRGQAAAIRALIALNKAQIPQSMEYANQALAYLPDNHFNRAVASLALGIAKG
                     VIGDFDGAIKIFLEARRDSLAVGNRILAQAVILQMGRVLAMQGRLHQAAEVFHEAVQL
                     TYRKTNLKIPYASGACVLLANIYREWNDLDTAYALLKEGIEIGVPSKMVDSVCIGNAI
                     LSQIYLAQGNIEEAERTSQEANRMIKTIPDPEPEAMTITLDCQVRLQLAAENYLEASK
                     LVREHGLDVRDEILYFHELEHIVLARVLIYSGRENPDNGHLLTDAQELIARMLEVAKP
                     AGCMREVTVLLVLQALAYQAQMNDDLALSSLAEALVLAEQEGYVRTFIDEGDQMQELL
                     RLASSRDIATHYIGKLLTAFGSGRLVTQQIASPLIEPLSERELEVLHLLATELSGPEI
                     ARELVISLNTMRTHTKNIYSKLNVNSRVAAINKARELNLL"
BASE COUNT         1708 a         1604 c         1414 g         1677 t
ORIGIN      
        1 taaggctaag tgtcagttca catgggcaca tccaatcacc gaccagccac gctctgctgc
       61 cagggcagcc agatgtgaat ccggatatac agcgatcgga ttgccgaaca tctcgaggac
      121 aggtgcatcc acgaaggcat cggtataaaa gtaagacttc tccagatcaa tttcatttcc
      181 tcggtcgttc agaaagcgct ggaggcgctc aactttacct tgcccaatat tcaggggagg
      241 gacgatcttt cccgtgtaat gtccatcctt cacgaccagt ggggtggcaa tagcgcattc
      301 aagccccaaa cggatggcta gtttgtcgag aaggggtgta aaagaacccg aaatcaagat
      361 gaccctgtgg acctggttcc ggtgatcctc gataacagct tgcatctcgg ggcgcagatt
      421 aggtaagatc tcgtgatcga taatccaatc ccaaatcctc tccgcccttt cgattgaaac
      481 tcctcccacc agccaggcaa ggttggttgc atttatccgg tagaaaaggt ccttaggtac
      541 tagacctgcc ttagccaact gccacagggg aaaatgaaaa gccacaaacc tagccacaac
      601 aaggcgtttg aatctgtttc gtctatggtg tgcaaaaagt gcctgccaaa ggaatttcgt
      661 ataaagcgta ttgtctaagt cgatgaaagc ggcgacatca ttttttagta cgctcatctg
      721 ctaagtcttt cagtctctag cattgcttac ttttattaaa ctggtatcca ggtatggtgt
      781 tgaagccata cctggattac acacaaggac atcatgcaat ggctgcggac agcctgagcg
      841 tatctcgaag agatctcaca atcgctccgg caccaaccaa gatgactacc attgctacaa
      901 aaaggccgat gatcggtatt gtgcgtacga tttcgtatat cagtacgccg atagccaatg
      961 cccacacatc accccaacgg ctcgcgagtg tgtcttggga aattcgcccc actacaagcc
     1021 tgccaagcat gtaagcaaag accgctttag ttaccagcga aatcacgaag acgaaaaaaa
     1081 ccacaaagga agctatggca aaaccgccga agccgataac aatgctagat agttgtccca
     1141 aagtgattaa accagttatg aaagccatcg caataactag agcgagtgta accataagaa
     1201 ctagagggaa gagcaagatc accagtagcc cccatccaaa gctgggcaat gcgctaccag
     1261 agatcttatc cataacactc cgaaccctat ctttaaggaa ataaacgagc agtccaccca
     1321 caattagcaa ggaaataaat tcacctatcg cccgtcgtat ttcttgaacg acaataaaac
     1381 gaagaacaga atttcgaagt tcttcgaaat caacctccat gggttggatt tcaatgttac
     1441 tggcatccac actctcgtaa ttcctcgcgg tcactttgta ggtgatctct cccccaaccg
     1501 tagaatcatc tgcgatcctc agaccgggtt gaatggcttc tacaggaaca aattcgaggc
     1561 aatatgggca ggtctcgccc aattcgacgt ccacattccc gccggctgac ccgttgaatt
     1621 cgaaggcacc ggtggcaacc tcaacatctt tcccgacctc cccattcagc ctgttttgga
     1681 aaaccgcagc atgaacatca tagcccacat gactgccact ctcggcgatc aggctgaaac
     1741 caccagcgta aacactgcga ccgatcgcag cctgcggtcc aagcgtgatc gcacatccac
     1801 cgctatacag gctgccgcca atatctccat tgatggttag aagctcgcct ccaatgaaaa
     1861 cgctgccttc tacgctgccg ttcaggacca cctcaattcc ggtcgcaaac aggttgccgg
     1921 tgatggtgcc atcaattacc aacttgtctc cagaaataat caggtcgtcg tcaatgacaa
     1981 cacccgcggc tacgactgca tccggatcac ccgtgacaac ctcagcagcc tgggcagaac
     2041 ctgcaaaagc taatgtcagg aacccaacca tgataaggac taatattgct aacctccccc
     2101 acttattttg aatagacgtt ttgaaattca tttcacattt ctcctctgtt ttgttagttg
     2161 caccggagct cctggtctct taggaggagt ctccggcaca tcgatagtgt caacaagcga
     2221 aatcttataa tacttgcgct cgcttatccg ctccttccag cggctggcaa tgaagttcca
     2281 tcatttggga catcaccttc agccattcat ctggaacttc tgcctggcct gtgatttgag
     2341 acatcatttc agcacaatca catcctgcca tgtgctctcc catcatcttc tccatcattg
     2401 ccatgcaggc agatatgtca aatggggttt gcttttcttg agttgttttt tgtttaaatg
     2461 tcatttcttg ctctcctatc ttgatttctg gtatgtaaaa ataattctcg ctctgatgaa
     2521 acgcactgcc cggaagcaaa accccaagca atgcgcttca tagagggatt cctaaccgtt
     2581 gctaatggcc ttgatcaagg tcactccgcc gatcaatacc agcacgagcg gccaaaacat
     2641 atcgatggcg aaaagtccac tcagaccgag taacagccaa aatccggacc agatgtaatc
     2701 ccaagtagtt gtcacagggt ctggtaaggt ggagtggtag atattcataa atgtcccgta
     2761 caaacccgca ccaatgaaca cccagctcca ggcatcacca ctgccgaatt gaggcaggac
     2821 gcccaagctg tttgccccaa ataccaggcc tgcccatacc aatacgccag cccaatatag
     2881 gctttcagcc atggagcgcc tctcaatctt gctaaaaaat tgttctgtga tcatttttac
     2941 ttctcctttc tgtttgtatt tttatcttct aggtactctt caattggatt gactgagatc
     3001 aatttgaggt tcatattacc gattttgagt aacaccccgt gtaaagcagc ttgatcggag
     3061 agtacgccag atagggttgt tgttccgtct gcgttccggc aaatcatcag cccttcgagc
     3121 cattcctccc agcgatcttg caggtgtcct tttatgagaa ttttgtaaaa ttctggcact
     3181 tttatctatt tccttacatt catgagttgc tcagaatata caaaaaaatg tggtgaggcg
     3241 tcatcaccac ttgatgtgat taacctggtg atttctggta agtaaaaatg atgtggcaat
     3301 ggtgaatcaa aaaagccccg aggggcaatt tttaaaattc ttccttgttt gcgtgatagg
     3361 ggggatcgct gtagcttata acaggtttag ctctcttgcc ttatttatag ctgccactcg
     3421 gctgtttaca tttaatttcg agtaaatgtt cttcgtgtgt gtacgcatgg tatttaggga
     3481 gatcaccaac tctcgcgcaa tctccggacc ggagagttcg gttgcgagca ggtggagaac
     3541 ttcaagttcg cgctcgctaa gcggctcgat gagtggtgag gctatttgct gcgtgacaag
     3601 cctgcccgac ccaaatgcgg tcaacaattt gccaatataa tgcgtggcga tgtcccgaga
     3661 cgatgccaag cgtagcaatt cttgcatttg atcgccctcg tcgataaatg tacgtacgta
     3721 gccttcttgc tcagccagca ccagcgcctc tgccaatgag ctcaacgcta gatcgtcatt
     3781 catttgggcc tgataagcca gcgcctgaag tacaagtagg acagtcactt ctctcataca
     3841 cccggcgggt tttgctacct ctaacattct ggcgattaat tcctgtgcat cagttaaaag
     3901 atgcccgtta tccgggtttt cacgacctga atagatgagc acccgggcta aaacgatatg
     3961 ctccaattca tgaaaatata agatttcatc acgtacatct aaaccatgct cccgaaccaa
     4021 ctttgatgct tcgaggtaat tttccgctgc cagctgtaat cttacttgac aatctaaggt
     4081 tatagtcatc gcttccggtt cagggtcagg aatggtctta atcattcgat ttgcttcctg
     4141 ggatgtcctt tcggcttcct ctatatttcc ttgagccaaa tatatctggg acaatattgc
     4201 attaccaatg caaactgaat ctaccatttt cgacggtaca ccgatctcaa tcccttcctt
     4261 taacagcgca taagcggtat ccaagtcatt ccattcccgg taaatatttg cgagaagtac
     4321 acaagctcca cttgcatatg gtatcttgag atttgttttt cgataggtta gttgaacagc
     4381 ttcgtggaat acttcagccg cttgatgcag ccgcccttgc atggccagca ctctacccat
     4441 ttgcagaatt actgcctgag ccaaaatgcg gttgcccaca gcaagactat cccttcgagc
     4501 ctcgaggaat atcttgatgg caccatcaaa atcaccaatc acccccttag cgatacccag
     4561 ggcaagagag gccacagctc gattaaagtg gttatcaggc aggtatgcaa gcgcctgatt
     4621 agcatattcc atggattgag ggatctgtgc cttattaagg gcaattagag cgcgaattgc
     4681 ggccgcctgt ccccgaatta catctttccc accaacgggt tctggagtgt tttcatcctc
     4741 tagagctcgt tcggcgtact gcaagttttc ttctgcttct tttaaacgag cccatctctg
     4801 acacatccaa gcccgaatga tgcaaagcca gggccaggcg acaaccattt gttgggggat
     4861 tgcatctagc cagctcgata actggtcaaa ctcgcttcgc atcatcatct cggggccgag
     4921 actttctact atccgggcag cctgctcgaa atcttctcct gccaacgaat ggttgatggc
     4981 ttcagtgagg aatttgttct tttctagcca tttggaggcc ctgcgatgca gtttgggaat
     5041 gctccctggt tctctttcgc ccaaacgctg gcgcagaaaa tcggcgaata aatgatgata
     5101 acggtaccag gtatgctcat catctaatgg aattaaaaac aaactagctc tatctaaagc
     5161 ttgcaggaca tcattactgt tggaaatttc taaaacatcg ttgcaaagag aagcactgaa
     5221 acgatccaag atggaggttt ggtacagaaa tgtttggatt ttttctatct gacgagccat
     5281 gacctcgtct actaggtaat cgataatata gtgatggctt cctgaaaatg cggtaataaa
     5341 ctcatgctta tctacgcgcc cttgcataga taaagccgcc aattgcaacc ctgtgatcca
     5401 gccttctgtg cgagtaagca gcgccagtat gtccccagtg gacaaatcca atccaaggag
     5461 atcatttaga aaagatctaa cttctgcttc tgtaaagcgc aaatcatcga ggcgtatttc
     5521 gaccatttga ctcctggcac ggtaacgtga tagaggtaat ggtggatcga ctcgtgtgct
     5581 tatcaacaag tgagcgttcg gaggtaggtg gtcgaggaag aaatttaggg cttcatttat
     5641 cttctgtgaa gtaatcagat gataatcatc gagcaccaat gaaaactcta cccctgactc
     5701 tgcaatctcg ttgatcaaga tggtgattgc aagttcaatc ggagggttgt ctgtcgattc
     5761 tagtgaagct agaatgctct ccccaatcga tccgtcaatt tgccggagtg cagcaatcag
     5821 ataagaaaga aagcgcttaa ggttattgtc attctcatct aaagagagcc aggtgacggg
     5881 tttatcgatg gtcctggccc attcgctcat tagagtgctt tttccaaaac ctgccggcgc
     5941 agatatcagg acaaacttaa cccccatacc agcattgagc cttgaaatca ggtgcgggcg
     6001 cgaaaccaag cctggagagg tttggggaat ataaagtttt gtggtcagaa gcggagtcgc
     6061 cacggatgaa accagatttt gtcagttagg tctattgaat tgatatatct tacacgattt
     6121 ttgtacattg cgcacgttat aaactgtctt gaggcagggc aattgcatat gccctgaggg
     6181 gcatgcgcaa tcccgtaaaa agagtgtttg gggtgctttg gtggggggat tcccccacca
     6241 aaacacccca ttttcattct tcagtggtgg aaaatcgccc aaatgcgatt gtcctactgt
     6301 cttgagcctg aaaaccaggg gaaggagaga aataatcaga taatatacct gtgttcagaa
     6361 tgtgtaacgc aggcattact tgacgatttt aattgtgaga att
//
LOCUS       DBNGSX010000297         2609 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_509645, whole genome shotgun sequence.
ACCESSION   DBNGSX010000297 DBNGSX010000000
VERSION     DBNGSX010000297.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_509645"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="AC16CB_09010"
     CDS             complement(<1..512)
                     /locus_tag="AC16CB_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342647.1"
                     /translation="MTNKLSRYCDGIMEAAWLAALILVPVYFNIYSSRIFEPDKIAIL
                     RSLALVILGAWIVKLFAEGGIRWERIQPGNSILKFITKFPLIAPVAALAALYLISTVF
                     SVAPRTSLWGSYQRLQGTYTTFSYLIIFVAILGNLRRQAQIDRIITTAVLTSLPVALY
                     GILQRYDIDP"
     gene            782..1642
                     /locus_tag="AC16CB_09015"
     CDS             782..1642
                     /locus_tag="AC16CB_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3342648.1"
                     /translation="MSSINKKERLLTGNYAHVVIEPPKGWSSLELSELWKYHELLFFL
                     TWRDIKVRYKQTMLGAAWAILQPVLTMIVFSIIFGGLAKLPSDGIPYPIFTFTALLPW
                     QLFAYALSQSSNSLVGSQNLISKVYFPRLVVPFSSVLAGVVDFAIAFLVLVGMMAFYG
                     IGLTPAALLLPLFVLLALASALAVGLWLSALNVQYRDIRYVVPFLTQFWMYATPIAYS
                     SSLIPENWRWLYSLNPMTGVVEGFRWVILGKSSLDVLSLGISACVVVLLLVGGLYYFK
                     RMEANFADII"
     gene            1644..>2609
                     /locus_tag="AC16CB_09020"
     CDS             1644..>2609
                     /locus_tag="AC16CB_09020"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015192319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3342649.1"
                     /translation="MSNVAIRVEHLGKQYRLGGRQASITTFREILVDSARAPLRWLKG
                     DRNAEQNLFWALDDVSFEVKHGEAVGIIGRNGAGKSTLLKIFSRITSPTRGRVDIFGR
                     VGSLLEVGTGFHSELSGRENIYLNGAILGMSRVEIGRKFDEIVDFSGIEKFLDTPVKH
                     YSSGMYVRLAFAVAAHLEPEILIVDEVLAVGDAEFQKKCMGKMGDVARQGRTVLFVSH
                     NMAAIQNLCRVAYLLSRGKLVFSGDRDIVINRYLEYTKSEMQQDLKNRTDRSGNQRLR
                     FTGLNLEDRDGNRVQEMVSGKDYSIVLSYESAGPLPENVSISIGVY"
BASE COUNT          641 a          564 c          710 g          694 t
ORIGIN      
        1 atgggatcga tatcatagcg ttgcagaatc ccatataacg caaccggcag gctggtcaag
       61 accgctgtcg taataattcg atcaatctgg gcttgccggc gaaggttccc caagatagca
      121 acaaatatga ttaaataaga aaatgtggta taggtaccct gtaagcgctg gtaagacccc
      181 cacaggctgg tgcgcggtgc cacagagaaa accgtggaaa tcaggtagag ggctgccaag
      241 gctgcgacgg gggctattaa tgggaattta gtgataaact taagaattga gttgcctggt
      301 tggatacgtt cccagcgaat cccgccctct gcaaaaagct tgacaatcca ggcacctaag
      361 ataaccagag ctaacgagcg caggatagcg atcttatccg gctcgaagat gcggctggag
      421 tatatattaa aataaaccgg tacaaggatc aaagccgcca gccaggcggc ttccataatc
      481 ccatcgcaat agcggctaag tttgttggtc atagattttg tggaaatatt gccttgattt
      541 gaattgagaa ttacgatatc acagatttga gaagcctgca aggattgtcc tcataagatc
      601 ttatgactgt catcttaatt atttatgcaa attatccgca atttcatttg gcgctctggg
      661 ttgtctgacg ataatcgagt taggcaaggg ttatcaaaaa ccgatgttgg ttggaaagtg
      721 tctatttttg cctagctgaa aaagaatata ttttctatga atttgaagga taatgactca
      781 agtgtcttcc attaataaaa aagaaagatt acttactggg aattacgcgc atgttgtcat
      841 tgaaccaccc aaaggctggt cgtcgcttga attgagtgaa ctgtggaaat accacgaact
      901 actttttttc ttgacctggc gcgatattaa ggtgcgctat aaacaaacca tgctgggcgc
      961 tgcctgggca atattgcaac ctgtgctgac catgatcgta tttagcatta ttttcggagg
     1021 tctggccaaa ctaccttcag acgggatccc atatccgatt ttcacgttta cggcactgct
     1081 accctggcag ctttttgcct acgcgctgtc acaatcttcc aacagcctgg taggcagcca
     1141 aaacctgatc agcaaagtct atttcccgcg acttgtggtg ccattttcgt cggtattggc
     1201 gggggtagtg gattttgcga ttgccttctt ggtattggtg ggaatgatgg ctttttacgg
     1261 tataggcctg actcccgccg ccctgctgct gcccctcttc gtgctcctgg cactggccag
     1321 cgcgttggcg gtaggcttat ggctttcagc actcaatgtg cagtatcgcg atatccgtta
     1381 tgtggtgccg ttcctaacgc agttctggat gtatgccact ccaattgctt attcgagttc
     1441 actgatcccg gaaaattggc gctggcttta tagcctgaac ccaatgacgg gcgttgtgga
     1501 gggttttcgc tgggtgatcc tgggcaagag cagtctggat gtgttgtccc tgggcatttc
     1561 agcctgcgtg gtagtcttgc tattagttgg cgggttatac tactttaagc gtatggaagc
     1621 caattttgcg gacatcatat aaaatgagca atgtagccat tcgtgttgaa catttgggga
     1681 agcagtacag gctgggcggg cggcaggcat cgattaccac attccgcgag atactggtgg
     1741 atagcgcccg agcaccacta cgctggctga aaggtgaccg caatgccgag cagaacctct
     1801 tttgggcatt agacgatgtc tcgtttgagg taaagcatgg ggaagcagtg gggattattg
     1861 gtcgcaatgg ggctgggaag agcacattac tcaaaatctt ttcgcgcatc accagcccga
     1921 cgcgtgggcg ggtggatatt tttgggcgcg tcggttccct gctggaagta ggcacaggtt
     1981 tccactctga attgagcggg cgcgagaata tttatctcaa cggggctatc ctgggcatga
     2041 gccgggttga gatcgggcgc aaattcgatg aaatcgtcga tttttcaggc attgagaaat
     2101 ttctcgatac gcctgtaaaa cattattcca gcggcatgta cgtgcgcttg gcttttgccg
     2161 tggccgccca tctcgagccg gagatcctga ttgtggacga ggtgctggcg gtgggcgatg
     2221 ctgaatttca gaagaagtgc atgggcaaga tgggtgatgt ggcgcgccag ggacggacag
     2281 ttttgtttgt gagccacaat atggcggcga ttcaaaactt gtgcagggtt gcgtatttgc
     2341 tctctcgcgg caagttagtc tttagtggcg acagggacat cgtaatcaac aggtatcttg
     2401 aatatacaaa atctgagatg cagcaggatc tgaaaaaccg cacagaccgt agcggaaacc
     2461 aacgtttgag atttactggc ttgaacctgg aagatcggga tgggaacagg gtccaagaaa
     2521 tggtgtccgg aaaggattat agcattgttt tgagttatga atccgccggt cctctgcctg
     2581 aaaatgtgtc catctccatc ggtgtttac
//
LOCUS       DBNGSX010000298         6299 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1202126, whole genome shotgun sequence.
ACCESSION   DBNGSX010000298 DBNGSX010000000
VERSION     DBNGSX010000298.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6299)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6299)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6299
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1202126"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            499..2076
                     /locus_tag="AC16CB_09025"
     CDS             499..2076
                     /locus_tag="AC16CB_09025"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIR3342650.1"
                     /translation="MPSDNSLILAIDLGTTGPKVALFTAQGELRGYEFEPTPYYLQPN
                     GGAEQKPEEWWRAICIATRRLLDRGLAPPEHIAVLCCTTQWSGTVAVDRDGTPLMDAI
                     IWMDSRGAPYVQRATHSPLSVQGYGIDKLITWIRLSGGIPSHAGKDPIAHILFIKHEY
                     PEIYRQTDKFLEPKDYLNLRLTGVCAASYDSITLHWVTDNRDLSRVDYSPELLRLGTL
                     EREKLPDLMPPLTILGTLTQHAADDLGLHTGVQVVIGTPDIPSAALGSGAVRDFEAHL
                     YIGTSSWIACHVPFKKTDLFHNIASLPSAIPGKYLVSDEQQTSGACLSFLRDNILFYH
                     DELFTEADVPDVYNIFNQIAARAPAGSGGVIFTPWLYGERTPSDDRYARGGFFNLSLQ
                     STREHIIRAVLEGVAYNTRWLFGYVEKFVGRSLDALNAVGGGASSDIWCQIMADVLGR
                     DIHQMKDPILANARGAAYLASVAMGYMTFDQISDQTQIKRTYAPNPENKLIYDAMFKE
                     FLQIYQRTKPIYARLNR"
     gene            2147..3622
                     /locus_tag="AC16CB_09030"
     CDS             2147..3622
                     /locus_tag="AC16CB_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR3342651.1"
                     /translation="MNPRWVSVIEKMLRAIPSFNKAVHNEIETLTTDLYASLKPYRND
                     FSSYECLPPIGRDRDEILAEMQAIIAREESTWRNGFVSGGVYHGEDTHIKFLNQVYAL
                     QSQANPLHADIWPSISKYEAEIVSMTAHMMNAGTLGENNDVCGTVTSGGTESILLAMK
                     TYRDWARERKGITRPEMVLPVTAHAAFDKASHYFNIKPVLIPIDSDYKADINAARNAI
                     TRNTVVLVGSAPSFPHGIIDPIHELSELALQHGIGFHTDACLGGFLLPWAERLGYTLP
                     PFDFRLPGVTSISADTHKYGYAPKGTSVVLYRSTEMRHYQYYTASEWPGGMYISPTIA
                     GSRPGALSAACWAAMVTMGEEGYLEAARKILQTAVSIRQGIESIPDLYVLGDPLFVIA
                     FASSSLDIYQVTEHMHRRGWNLNRLHKPISVHLCVTLAHTQPGVAERFITDLKASVDE
                     ARLFPEKKTGMAPIYGMTASLPLRGVINDVLKRYIDALYKV"
     gene            3712..4653
                     /locus_tag="AC16CB_09035"
     CDS             3712..4653
                     /locus_tag="AC16CB_09035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3342652.1"
                     /translation="MILITGGTGFIGKALIRNLVESGNVVRTLIRPSARSPQLPHGVP
                     VEVAVSSLSDARSLRAAMVGVKAVYHLAGVERRGAYADLMAVDIQGTRLVAQAAADAG
                     VERIFFLSHLGADRASAYPVLKAKAIAEEYVRRSQVDYTILRSAIIFGPNDGFTTGLA
                     GLLSTIPYLFFYPGDGRTLIQPLWVEDLVTCMTWALEDDATRNQTYELGGPEYFTFLQ
                     VLEMVMQSLGLRRRLVSMRPPYLRAFTVALEALFPGLPVSVYWLDYLAANRTCALDTI
                     PRTFHLMPTRMSQRLDHLRGVNWRVSMLRNLLRRSPR"
     gene            4674..5588
                     /locus_tag="AC16CB_09040"
     CDS             4674..5588
                     /locus_tag="AC16CB_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342653.1"
                     /translation="MTIQLRILETPEEIVLVEELQRQVWPGSETEIVPAHVLLTAIHN
                     GGLVIGAFSDDREPGVQSLVGFVFGFPGLYFTPDGPRPKHCSHMLGVLPEYRDLGVGF
                     TLKRAQWQMVRHLGLDRITWTYDPLLSRNAYLNITKLGAVCNTYRRNEYGQLRDGINM
                     GLPTDRFQVDWWVNSQRVNHRLSRRARQPLDLAHYFAGGAEVINPSEIGTDGLPRPAA
                     MDHYEPLPRSADSGRPEVVPLLLVEIPADFMELKAQNFDLALQWRLHARNLFEDLFNR
                     GYLVTDFVFLKGSLPRSFYVLSFGESTL"
     gene            5585..>6299
                     /gene="menC"
                     /locus_tag="AC16CB_09045"
     CDS             5585..>6299
                     /gene="menC"
                     /locus_tag="AC16CB_09045"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HIR3342654.1"
                     /translation="MKIDRVALYHVRMPLVSPFETSFGRSETRDCVIIQVFADGLVGF
                     GECVADRDPGYSYETTGTAWHVLQEFLVPLLVGKSFSHPTDFQAAFDQVRGHLMVKAG
                     VEMALWDLQGKALGRSLHQLLGGVHPRVAVGVSVGIQPSPAALVKVVEEYVHQGYQRI
                     KIKIKPGHDVSETQAVRRALPHIRLQVDANSAYTLESAQVLLPLDELNLLLIEQPLFE
                     DDLWDHSRLQAQFTTPICLD"
BASE COUNT         1456 a         1765 c         1558 g         1520 t
ORIGIN      
        1 tggttattag aacaatctgt ggataccgtc acgtttgaaa gctacccatc cccttggttc
       61 tatccatacg aatattgggg ataattgctc tacagcagta tccacaatac aagccccgat
      121 gaaacctccc ttataatatt ataatagtta attatttcac aaatgtcaag attcaatcag
      181 tgaggttcag actgaatttc agacactctc caaaagatag catcgcaagc gctagcgaat
      241 cgatcttagt aaaattttat gaaattgcca ccctcaataa agctcaggga aggcgcccct
      301 ttgaggctca caatgaccac ttaagacaag gttacctttg atcgtccaaa ttattatcta
      361 aagatcagcc aaccatacac cgaagataaa acctataatg tcagtttttc tctaacaaat
      421 attaacgctt gtatcgctat cgcttgaata cacacaactt tcatgctaaa ctgaacgagg
      481 tgggagatca tagcactcat gccatcagat aactcattaa tccttgccat cgacctgggt
      541 accactggtc caaaggtcgc cctgttcaca gctcaagggg agttgagggg ctacgaattc
      601 gagcccacac cctactatct gcagcccaat ggcggcgccg agcaaaaacc tgaagaatgg
      661 tggcgagcaa tttgcattgc aacccggcgt ttgctggacc gcggcctggc acctccggaa
      721 catattgctg tactatgttg caccacccag tggtccggta cagtggctgt ggaccgggat
      781 ggcacgcctt tgatggatgc catcatttgg atggattcgc gcggtgcccc ctacgtccag
      841 cgcgccaccc atagcccact atctgtgcaa ggttacggta tagacaagct catcacttgg
      901 atccgcctat ctggaggtat cccctcacac gcgggcaaag atcccatagc ccacatcctg
      961 ttcattaaac acgaatatcc tgagatctat cgccagactg ataaattttt agagcctaaa
     1021 gattacctga acctgcgcct gaccggcgtt tgtgccgcgt cttacgactc tatcaccttg
     1081 cattgggtta cagataaccg ggatctctcc cgcgtggact actctccaga gttgttgcgc
     1141 ctggggaccc tggagcgcga aaaactaccc gacctgatgc ctcctttgac cattctcggc
     1201 acgctcaccc agcatgccgc cgacgacctg gggctgcaca caggtgtcca ggtggtgatc
     1261 ggcacaccag acatcccgtc cgccgcctta ggatctggcg ctgtccggga ttttgaggcg
     1321 cacctctaca ttggcacatc ctcctggata gcctgccacg tgcccttcaa aaagactgac
     1381 ttattccaca atatcgcttc cctgccatct gctatcccag gaaagtatct ggtttcagat
     1441 gagcaacaga catccggcgc ttgccttagc tttctgcgag ataacatcct cttttaccat
     1501 gacgagctgt tcaccgaggc ggatgtgcca gatgtgtaca acattttcaa ccagattgcc
     1561 gctcgcgctc cagccggtag cggtggcgtg atattcaccc cctggctata cggtgaacgc
     1621 acacccagcg atgatcgcta cgcgcgcggt gggtttttca acctgtcgtt gcaatctacc
     1681 cgtgagcaca tcattcgcgc cgtgctagaa ggtgtggcat acaatacgcg ctggctattt
     1741 ggctatgtgg aaaaattcgt tgggcgtagt ctggatgcgc taaacgcagt cggtggtggt
     1801 gccagctcag atatctggtg ccagataatg gccgatgtat taggccggga tatacaccag
     1861 atgaaggatc ctattctggc caacgcccgc ggcgcagcat acctggcctc ggttgccatg
     1921 gggtatatga cctttgatca gatttctgac cagacacaga tcaaacgcac atatgctcca
     1981 aatccagaga acaagctcat ctacgacgcc atgttcaagg agttcttgca aatctaccag
     2041 cgtacgaagc caatttacgc tcgcctgaat cgatagcagc cagtaaacaa ggcaatagag
     2101 gtgcaccttg aaatataaag actcaattct tgccatcctc ggacgcatga acccacgctg
     2161 ggtgtcagtg atcgaaaaaa tgttgcgggc gataccttca ttcaataaag cagtgcacaa
     2221 tgagatcgag acgctcacca ccgatctata cgcttcactt aaaccctacc gtaatgattt
     2281 cagcagttat gaatgtcttc cacccattgg tcgtgaccgg gatgaaatcc tagcggagat
     2341 gcaggcaatc atagctcgcg aagaatctac atggagaaat ggtttcgtgt cggggggtgt
     2401 ttatcacggc gaagacacac acatcaaatt tctcaaccag gtttacgccc tgcaatccca
     2461 ggccaatcca ttgcacgccg acatatggcc tagcatcagc aagtatgagg ccgagatcgt
     2521 ctctatgaca gctcatatga tgaatgctgg aacgcttggt gagaataatg atgtttgcgg
     2581 cacggtaact tccgggggta cagagagcat cctgctcgcc atgaaaacct atcgagattg
     2641 ggcacgcgag cgcaagggta tcacccgacc cgaaatggtg ttgcccgtta ccgcacatgc
     2701 tgcctttgac aaagcatccc attatttcaa catcaagcca gtcttgatac caattgattc
     2761 cgattacaaa gctgatatca acgccgctcg taatgcgatc acgcgcaata cagtcgttct
     2821 ggtaggctca gcaccatctt ttccccacgg cattatcgat ccgatccacg agctatccga
     2881 actagccctg caacacggta ttggttttca caccgatgcc tgcctgggag gattcctgct
     2941 gccttgggca gagagacttg gttacaccct tccgcccttc gattttcgcc tgcccggcgt
     3001 gacctccata tctgctgaca cgcataaata tggttacgct cctaaaggca cctctgttgt
     3061 cctgtaccgc agcactgaga tgcgccatta ccaatactat accgcttccg aatggcctgg
     3121 aggcatgtac atctccccga ctattgccgg cagccgacca ggtgcactga gcgcggcgtg
     3181 ctgggctgct atggtcacca tgggtgaaga aggctatcta gaggctgccc gaaagatatt
     3241 acaaaccgca gtttccatcc gccaggggat tgagtcaatc cctgatcttt atgtgcttgg
     3301 agatccgctg tttgtcattg ctttcgcctc atctagcctc gatatttacc aagtgaccga
     3361 gcacatgcac cgccggggct ggaacctcaa caggctgcat aagccgatca gtgtgcatct
     3421 atgcgttacc ctcgcgcaca cgcagccagg tgtagccgag cgttttatta ctgacctcaa
     3481 agcctctgtt gatgaggctc gcctgttccc agaaaaaaag accggcatgg ccccgattta
     3541 cggcatgact gcctccctgc cgctgcgcgg cgtaatcaat gatgtattaa aaagatacat
     3601 cgatgccctt tacaaggtct aatcaaaagc tgtgatgatg gcgcttgggg gacgtgcacc
     3661 tatcatggta gacttgatct aaacacaaat aaggcataaa atcctcctgc gatgatatta
     3721 attactggtg gaactgggtt tatcggcaag gcgctcattc gcaaccttgt cgagagtgga
     3781 aacgttgtgc gcacgctcat ccgcccttcc gctcgctcgc cacaattacc ccacggcgtg
     3841 ccagtggaag tcgcagtgag tagcttgagc gatgcgcgca gcttgcgcgc ggcgatggta
     3901 ggtgtaaaag ctgtctatca cctggctggt gtggaacggc gtggagctta tgccgattta
     3961 atggccgtgg atattcaggg cactcgctta gtcgcccagg cagccgctga tgccggtgtg
     4021 gaacgcatct ttttcctaag ccaccttggt gcagatcgcg cctccgccta tccagtactc
     4081 aaagccaaag ccatcgctga ggagtacgta cgccgcagcc aggtagatta caccattttg
     4141 cgctccgcca taatatttgg accgaatgac ggtttcacga ctggccttgc cgggctgctc
     4201 agcaccatcc cttatctttt cttctacccc ggtgatggtc gcacgctcat ccagcctttg
     4261 tgggtcgagg acctggtcac ctgcatgacc tgggcactgg aagacgatgc tacccgaaac
     4321 cagacttacg agctcggcgg gcctgagtat ttcacctttc tgcaggtcct tgagatggtc
     4381 atgcaatcgc ttgggctgag acgccgtttg gtgtctatgc gccctcctta cctgcgagca
     4441 ttcaccgtag cactcgaagc ccttttccca ggccttccgg tatctgtcta ttggttggat
     4501 tatttagcgg caaaccgcac ctgtgcccta gatacgatcc cgcgcacctt tcaccttatg
     4561 ccaacccgca tgtcgcaacg cctggatcat ctgcgggggg tgaattggcg ggtctccatg
     4621 ctgcgaaacc tgttgagaag atctcctcga tgagtagagg tggtttgact aagatgacaa
     4681 ttcaattgcg gattttagaa acgccagaag agatcgtctt ggtcgaggaa ctccagcgcc
     4741 aggtttggcc gggcagtgaa acagagatcg ttcccgccca tgtcctgctg actgccatcc
     4801 acaatggcgg gttggtgatt ggagcctttt ccgacgatcg tgagcctgga gttcaatcgc
     4861 tggtcggatt tgtttttggc ttccccggtc tctactttac ccctgatggg ccgcgaccca
     4921 agcactgttc ccatatgttg ggtgtcctgc ctgaatatcg cgacctaggg gtaggattca
     4981 ccctcaagcg cgcccaatgg caaatggtgc gccaccttgg tttggaccgt atcacatgga
     5041 cctacgatcc tctactcagc cgcaatgcct atttaaacat aaccaagctg ggcgcagttt
     5101 gcaacacata ccggcgcaac gaatatggtc aattgcgcga cgggataaat atgggcttgc
     5161 ccactgaccg attccaagtg gattggtggg tcaactcaca acgtgtcaac caccgcctca
     5221 gccggcgcgc tcgccaacct ctcgacctgg ctcattattt tgctggcggc gcggaagtga
     5281 ttaacccttc agagatcggc accgacgggc taccccgacc ggctgcaatg gatcattatg
     5341 aacctctccc ccgctctgcc gattctggca ggccagaagt agtccccctc ttgctcgtcg
     5401 aaatccccgc ggatttcatg gaactcaaag cccagaattt cgacctggca ttgcagtggc
     5461 gtttgcatgc ccgcaacctg tttgaagatt tattcaaccg cggatacttg gttacagatt
     5521 ttgtctttct aaaaggtagc cttccgcgca gtttctacgt tttgagtttt ggagagagca
     5581 cactatgaaa atagacaggg ttgccctgta ccacgttcgc atgccgcttg tatccccttt
     5641 tgagacctcc ttcgggcgca gcgaaacacg cgattgtgtc atcatccagg tatttgcaga
     5701 cggtctggta ggctttgggg agtgcgtcgc tgaccgggat cctggctact cctatgaaac
     5761 caccgggaca gcgtggcacg tccttcaaga atttctcgta ccactactgg ttggaaaatc
     5821 cttttcccat cctacagatt tccaagcagc ttttgaccag gtgcgcggtc acctgatggt
     5881 aaaggctggg gtggagatgg cactctggga tctgcagggc aaagctctgg ggcgttctct
     5941 gcaccagcta ctgggcgggg tgcaccctcg cgtggctgtg ggtgtctcgg ttggcatcca
     6001 gccctccccc gcagccctgg tcaaggtagt cgaagaatat gtccaccagg gctaccagcg
     6061 gatcaaaatc aagatcaagc ccggtcatga tgtgagtgag actcaggctg tgcgccgtgc
     6121 gcttccgcat atcaggctgc aggtagacgc caactcagcc tacactctcg aaagcgccca
     6181 agttttgctg cccttggatg agctaaactt gctgctgatc gagcagccgc ttttcgagga
     6241 cgatctctgg gatcacagtc gccttcaggc gcaattcact acccccattt gtctggacg
//
LOCUS       DBNGSX010000299         3820 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1340301, whole genome shotgun sequence.
ACCESSION   DBNGSX010000299 DBNGSX010000000
VERSION     DBNGSX010000299.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1340301"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2640
                     /locus_tag="AC16CB_09050"
     CDS             <1..2640
                     /locus_tag="AC16CB_09050"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3342655.1"
                     /translation="FQAIQRGTYHPDQHLPSTSSVRRTLLPQPTALPLVGRQQELARL
                     HDALQNARQGRGNFILISGDSGIGKTRLVQEYLSLYPGLLTLQGVCHELESVVPYASL
                     RQALVEAGNFLSPSMLKTPPSWLVTLGYFIPSLTTQLPYLPSRAPMQEESPRLLEALM
                     NLTMTINTGTPDQPLHLIMDDLQWCDGPTWDFLLQLSHQAENQPLLIYGLCRLEDLPR
                     ERSRLLRALERNELLLHLPLSRLSIEETAELSGYMLPQEESDPIFINRLYKETEGNPF
                     FIIEMVRSMQEAGHSMPSMDRVQGRQDHNLPLGIQRVIEARLDRLSPKSQEFLAISAA
                     IGTAFSFSWLLEIAQVTAEEGISYIEEWLHRGLVHEVGQTYDFNHEKIRQVAYNSLSY
                     ARRQYVHRRIAEVFANTVPPADPATLAYHYARSDQPLKALPFLTQAGEAALRVRSYQE
                     AYQLGLRAVSLLGRMPGPQQRSERIDLNLQLAQAYAFTNDLQRAQEILTETEHLAASL
                     GEEERLGKVYHRLAQIYWLRGQPEVAGDYARRTLRIAEDTNNLSLQMSSLRMLGRVGI
                     ALSAFDDAISNLLRYVRLENETAPTSDLPIVLGYLGIAYDRVGSWKRALETARRGLEM
                     AEAEGSTQAVAFARMQLGFVYADSQRWQECLDTLKAIPDPLDPTLSLLDRDADNHNLT
                     PLGFMLLGLRGRALAHRGDVRAGIELIQSGLTWAEKNNYRVFHYMPRLFLVESLYLEQ
                     NSNAVDEANLALQQAREAGNRWVVGVLLRILAESLFRKPYPDWSLIEKYLIESMNLLR
                     QVRARPELARTYLALRRLYDRAGQIAWAVDCHFRATSIFEELGMTDELRLAQGQAAGE
                     RRGAVVVSDMRLIGPNAGQESIN"
     gene            2702..3571
                     /locus_tag="AC16CB_09055"
     CDS             2702..3571
                     /locus_tag="AC16CB_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3342656.1"
                     /translation="MLRGFRHQLNSTQLNPGDLQLDIQDVQLSFLGVTALLNVSLNVH
                     QSEILAIIGPNGAGKTSLLNCISGLYHPQVGRIIYYNSGQHDVTKLRPYQIAKLGIAR
                     SFQNIELFRHMSVLDNLLAGCHVYMRSNLLSSMIYWGSAQREQIEFRQFVEDIIDLLE
                     IEAIRKQTVGSLSYGLQKRVELGRALAMRPSILLLDEPMAGMNSEEKEDMARFVLDVN
                     EEHGVTIVLIEHDMGVVMDISDRVVVLNFGQKIAEGTPDEVRRDPHVIQAYLGEDKSV
                     FPFKYGQQNNNNG"
     gene            3564..>3820
                     /locus_tag="AC16CB_09060"
     CDS             3564..>3820
                     /locus_tag="AC16CB_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3342657.1"
                     /translation="MAETLPQFLIEHARQTPNKYAMRERNYGIWQAITWGQYLEHVKH
                     FCLGMVSLGLQPEEAIAIIGDNRPEWVIAELAAQSAGAKSIG"
BASE COUNT          903 a          962 c         1010 g          945 t
ORIGIN      
        1 ttccaggcga ttcaacgcgg tacttatcat ccggatcagc atctcccttc gacatcttct
       61 gttagacgca cactgctacc ccagccaact gcgttaccct tggttggccg acagcaggag
      121 ctagcccgac tgcacgacgc gctgcaaaac gcccggcagg ggcgtggaaa ctttatcttg
      181 atcagcgggg attctgggat tggaaagacc cgtcttgtac aggaatatct ctcactttat
      241 ccgggtttgc taacactcca gggtgtttgc cacgagcttg agtcagttgt gccttacgcg
      301 tcgctgcggc aagcccttgt tgaggcaggc aattttctgt cgccaagtat gctgaagacc
      361 ccgccaagtt ggttggtcac gctcggttat tttattccat cattaaccac tcagctacct
      421 taccttccat cgcgtgctcc catgcaagag gaaagtcctc gcttgctcga agcgcttatg
      481 aatttgacta tgaccataaa cactggcact ccagatcagc cattacactt gatcatggat
      541 gatttacagt ggtgtgatgg ccccacctgg gatttcctgc tacaactttc ccaccaggca
      601 gaaaatcagc cattgttaat ttatggttta tgtcgactag aagatcttcc ccgtgagcgg
      661 agccgccttt tgcgtgccct ggagcgcaac gagctcctgc tccacctgcc actctccagg
      721 ctatcgatag aagagacagc agaactttct ggttacatgt tgccccagga ggaatccgac
      781 ccaattttta tcaatcgcct gtataaagag accgaaggaa atccattttt tatcatcgag
      841 atggtgcgtt ccatgcaaga ggcgggtcat tcaatgcctt ccatggatcg cgtgcaagga
      901 cgccaggatc acaacctgcc tctgggtatc cagcgggtaa ttgaggcgcg ccttgaccgc
      961 ctctcaccga aaagccagga atttttagcc atatccgcgg cgatcgggac tgcttttagc
     1021 ttctcttggc ttcttgaaat tgcccaggtt acggctgagg agggcatctc atacattgaa
     1081 gagtggttgc atcgaggcct ggttcacgag gtaggccaaa cctatgactt caatcacgaa
     1141 aagattcgcc aggtcgccta caacagcttg agttatgcac ggcgacagta tgttcaccgt
     1201 agaattgccg aagtatttgc aaacaccgtt ccccctgctg atccagctac attggcttac
     1261 cattacgccc gcagtgacca gcccttaaag gctttacctt tcctgaccca ggctggtgaa
     1321 gcggcattgc gagtgcgttc atatcaagaa gcataccagc taggcttacg cgctgtcagc
     1381 ctgctcgggc gcatgcccgg cccacagcaa cggagtgagc gcattgatct taacctgcaa
     1441 ctagcccaag catatgcctt tacaaatgac ttgcagcggg ctcaggaaat cctaaccgag
     1501 accgaacatc tggctgccag tctgggcgag gaagagcgtt taggaaaggt gtatcaccgg
     1561 cttgcacaaa tctactggct gcgtggccag cctgaggttg ccggagacta tgcccggcgg
     1621 actttacgga tagcagaaga caccaataac ttatccctgc aaatgagcag tctacgtatg
     1681 ttaggtcggg taggaatcgc cctatctgcc tttgatgatg ccatttccaa tctgcttcga
     1741 tatgtccgtt tggagaatga gactgctccc acctcagatc tcccgattgt attaggttac
     1801 ctggggattg cttatgatcg agtcggatct tggaaacgtg ccctggagac tgctcggcgt
     1861 ggcctggaga tggcggaggc agagggttct acccaggctg tggctttcgc tcggatgcag
     1921 ttaggcttcg tgtatgcgga tagccagcga tggcaggaat gcctagatac cctaaaggct
     1981 atcccggatc ctttggaccc gactctatct ctattggaca gggacgctga caatcataat
     2041 ttgaccccct tgggatttat gctcttaggg ctccgcgggc gtgctttagc tcatcgtggt
     2101 gatgtccgtg ccggaataga gctgatccaa tccggattga cgtgggcaga aaagaataac
     2161 tatcgcgttt tccattacat gccgcgcttg ttcctggttg aaagcttgta tctggaacag
     2221 aattcaaatg cagtggacga agccaatctt gccctgcaac aagcccgaga ggcgggaaat
     2281 cggtgggtag ttggggtttt gctgcgtatc ctggctgaaa gtctattccg caagccatat
     2341 ccagattggt cgctaattga aaaatatctg atcgaatcga tgaatttgct acgccaggtg
     2401 cgtgcgcggc ctgaattggc gcgcacatat ctggcactgc gccgtctgta tgaccgtgct
     2461 gggcagattg cctgggcagt ggattgccat ttccgggcta ccagcatatt cgaagagttg
     2521 gggatgacgg atgagctgcg tttagcgcaa ggtcaagcag ctggtgagcg ccggggggcg
     2581 gtggttgtct ctgacatgcg actcatcggg ccaaacgcag ggcaagaatc aataaattga
     2641 agcaagtatt aacgcccctg ttaacgcttt gatagcatca aataagtaaa attgatgcat
     2701 tatgctgcgc ggctttcgcc accaattgaa cagtacccaa ctgaatcctg gagatcttca
     2761 attggatatc caggatgtcc agctcagttt tttgggggtt acagcattat taaatgttag
     2821 cctaaacgtc catcaaagcg agatacttgc cataattggc ccaaatggcg ctggcaaaac
     2881 cagcttattg aattgcatta gtgggctcta tcaccctcag gttggtcgca tcatttatta
     2941 caacagcggg cagcatgatg taaccaagct gcgcccgtac cagattgcta aacttggtat
     3001 cgcgcgttcc ttccagaata tagagctttt tcgacatatg agcgtgctcg ataatctttt
     3061 ggcaggatgc catgtttaca tgcgctccaa tttgctctcg agcatgatct attggggttc
     3121 ggcgcaacgt gagcaaatag aattccgcca gtttgtagaa gacattatcg atctgctgga
     3181 aattgaggcg atccgcaagc aaactgttgg ctcgctttct tatgggctgc aaaaacgagt
     3241 cgaattgggg cgagcattgg cgatgcgccc ctccatcctg ctgttagatg agcctatggc
     3301 tgggatgaac agcgaagaga aagaagatat ggcgcgcttc gttctggacg tcaatgaaga
     3361 acacggtgtg accattgttc tgatcgagca cgacatgggt gtggtgatgg atatcagcga
     3421 tcgagtagtg gtgctgaact tcggtcagaa gattgccgag ggcacgccgg atgaagtccg
     3481 ccgtgacccc catgtcattc aggcatacct gggcgaagac aaaagtgtct ttcccttcaa
     3541 atacgggcaa cagaataata ataatggctg agacgctccc ccaattcctc atcgaacatg
     3601 ctcgccagac tcccaataag tatgcaatgc gggagcgaaa ctacggcatt tggcaggcaa
     3661 tcacctgggg acagtacctg gagcatgtca agcacttctg tcttggaatg gttagtttgg
     3721 gcctgcagcc cgaggaggcg attgccatta ttggggataa ccgcccggag tgggttattg
     3781 ctgaactggc agcacagtct gcgggggcga aatcaatcgg
//
LOCUS       DBNGSX010000300         6124 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_232105, whole genome shotgun sequence.
ACCESSION   DBNGSX010000300 DBNGSX010000000
VERSION     DBNGSX010000300.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6124)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_232105"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="AC16CB_09065"
     CDS             complement(<1..959)
                     /locus_tag="AC16CB_09065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR3342658.1"
                     /translation="MSPTDDFYGLLSSLLQQTAQNPDAPLAIPLNRWPAGSPEQRVLE
                     EFQAALAALQSGSQNRSSQIEESERRYQSIFEIVSDGLIIHDLVTGLVVEANPAACSM
                     YGYTRDEFIGLHPTKFIHPDSSNQYAKYVQAIQSQGANIAQQVHMRRDGSQFNVELSG
                     TVYADKGRSYLLSVVRDVSQRVHAEKLLQQRVEARTREQSTLLEISHTLTSALELQPD
                     LILDQLRVIVDYTHAGLFKLEDSTLAALAVRGPQSLEQAMPFQIRLQSPEALTMLFNE
                     RRPTRIADVQSSDPSAQFLHLLLYDQASILLEGMRSWMWVPLA"
     gene            1306..1734
                     /locus_tag="AC16CB_09070"
     CDS             1306..1734
                     /locus_tag="AC16CB_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3342659.1"
                     /translation="MFWPHLIAIARVYEPDVQLLRVSEPFDVTARLRLIDPVDWQIRK
                     AEAKSYLSGIAALLQNAGLWVSTHLYDGRPAEQIIEVAHSWNTDLILLSSHRQSGISP
                     WNVSSVVRQVMVYAGLGIAQSHFFRNSVCTRLASSNSSPG"
     gene            1864..2304
                     /locus_tag="AC16CB_09075"
     CDS             1864..2304
                     /locus_tag="AC16CB_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342660.1"
                     /translation="MVITDYYWPDQDNTARAFIYEEGGTIVVNPGLSAEQQARAKRMI
                     DLVGLDKNPGNDPAASDRRWTNRLEAWGMAKRARQHVQKFPVPEMRDQVVDTALAKGF
                     RSIWMTVFQDDPIMRVSFINSFKGTSATSFGPGANPIHRPNGDL"
     gene            complement(2806..3735)
                     /locus_tag="AC16CB_09080"
     CDS             complement(2806..3735)
                     /locus_tag="AC16CB_09080"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014114.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HIR3342661.1"
                     /translation="MTLLAPRLHIGDTIGIFTPSGEIDDSPSESPYEELEKGTRFLEK
                     LGFKVVLGENALKRGAFGAGTPQERADDINHMFANPDIHALISSHGGINANSILPYLD
                     YDLIRRNPKILLGFSDVSTLHLGIYSQTGLVTFHGNMVIWYFGMDPQEYDRQEFLDRL
                     VTGKIGPVNKHSEWRTVRGEKSAEGKLLGGNDWVMSWLLGTPYFPDMSDSILFIEIPG
                     FAPGSFYNRLTQFKQAGIFEKVRGVLIGFANEKDGYKAEDIFLEVTAEYDFPIVKTDD
                     FGHHCPNTTLPVGARCRLDPASARLEILEACVK"
     gene            complement(3974..4165)
                     /locus_tag="AC16CB_09085"
                     /pseudo
     CDS             complement(3974..4165)
                     /locus_tag="AC16CB_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611774.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016893 - obsolete endonuclease activity,
                     active with either ribo- or deoxyribonucleic acids and
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            4266..5555
                     /locus_tag="AC16CB_09090"
     CDS             4266..5555
                     /locus_tag="AC16CB_09090"
                     /inference="COORDINATES: protein motif:HMM:NF013226.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HIR3342662.1"
                     /translation="MKHTEVKSTITCDMEGRIETYNKGAEEIFGYSAEEVIGHKRVSL
                     FSPGLIVLEHVPTWLKTASETGEYKGQTVFVRKDGSTFNAEIRITPTFRDGKQVGYCG
                     VTKPLDPSQRANAMPKISMLTKVFAGMVITRMPFLTASLIPVLIGATWAVVARSASPF
                     PWLVFILTLLGAAALHVAANTFNDYFDWTSGTDKVNNDYFLPFSGGSRSMELGLISEK
                     GVLNVGLVALGLAFGFGLTVMFLSQQPLLILFGLAGAFSAYFYTAPPLRLVARKGLGE
                     LLIGLNFGPLMTAGTVLALTGRVAWEDFVIGLPVGLLTTAILWINQFPDEIADRKSGK
                     HNLVVVLGTERARWGYLTLLVAAFGISIICTLAGLFPIGTLAMLGGVPLAVSASRILF
                     QHYKDRELVSANAKTINLQLIAGLLTAAGILISPLIG"
BASE COUNT         1538 a         1491 c         1495 g         1600 t
ORIGIN      
        1 atggccaggg ggacccacat ccacgaccgc attccctcca ggagaatgct cgcctggtca
       61 taaaggagta agtggagaaa ttgtgccgac ggatcagagc tttgcacatc ggcaatccgg
      121 gtaggccggc gttcgttgaa cagcatcgtc agagcctccg gactttgcag acggatttgg
      181 aaaggcatcg cctgctccag cgactgcggc ccgcgcacag ccagggccgc cagggttgag
      241 tcctccagct taaaaagtcc agcgtgcgta taatcgacga tcacccgcag ctggtcgaga
      301 atcaggtccg gctgcagctc gagagcggaa gtcagcgtat gggagatctc taataaggtg
      361 gactgttcgc gggtgcgtgc ttccacccgc tgttggagca gcttctctgc atggacccgc
      421 tggctgacgt cacgaacgac actcaggaga taggagcggc ccttatctgc atatactgtc
      481 ccgctcagtt ctacattgaa ctgtgagcca tcccggcgca tgtgaacctg ttgcgctata
      541 tttgcgccct gagattgaat cgcctggacg tacttggcgt actggttaga gctgtcaggg
      601 tggataaatt tagtcgggtg caggccaatg aattcatcgc gagtatagcc gtacatcgaa
      661 caggctgcgg gattggcctc gaccacaagg ccggtcacta aatcatggat tatcaacccg
      721 tcgctgacga tttcgaagat actttgataa cgccgttcac tttcttcaat ctgggatgag
      781 cgattttggc tcccgctttg gagcgctgcc agcgcagctt gaaattcttc cagtacgcgc
      841 tgttctggtg atccagcggg ccagcgattt agaggaatag cgagaggcgc atccgggttc
      901 tgagccgttt gctggagcag gctggacaac agcccataga aatcgtccgt tggcgacatg
      961 aatatagttt accctatgca ggcgcggttg tcgtcctatg tcatttccgg gagtgaataa
     1021 tatttgggct ttcactccaa attcaaggat aaattcacga aaaagcccgt gaccgtatcc
     1081 actctcacat attctaactt aggttcctta cagtgaggta aaatcaattt cccagaaaag
     1141 ggaaacaaaa cctttcgtct ctaactacct aaatagctcc ctaaagcccc ttacaaccaa
     1201 gtacctggac aattggcctt tttcttataa agttattgtc aataaaggaa agaccgatgt
     1261 ttaaacggat catcctaccg ttggatagtt caaaattggc ggagtgtgtt ttggccgcat
     1321 ctgatcgcaa ttgcccgagt ctatgagcca gatgtgcagt tactgcgcgt ctcggaacct
     1381 tttgacgtaa cagcgcgctt gcgcttgatt gacccggtgg attggcagat tcgtaaagcc
     1441 gaggcaaaga gttatctctc tggtatagct gctctcttgc agaatgccgg tctgtgggtg
     1501 tccacgcatc tttatgatgg cagacccgca gaacagatta ttgaagttgc ccactcttgg
     1561 aatacagact tgatcttgtt gagcagccat aggcagagcg gtataagccc ctggaatgtc
     1621 agcagcgttg tgcggcaggt catggtttac gccgggttgg gcatagctca gagccatttc
     1681 ttcagaaact cggtttgcac tcgacttgcc tcatcgaaca gctcgcctgg ataatagagc
     1741 agcccaaaat gccccccagc aatatcgtac cactgcttgg gtccggtaat gagatcgtaa
     1801 gcctgcctag cgaccgagta attggcgtgc accaactgta attctacgaa gagcaataaa
     1861 gctgtggtca tcacagatta ttactggcct gaccaagaca acaccgcccg cgcttttatc
     1921 tacgaggagg gcggaacaat cgtagtaaat ccgggcctga gtgcagaaca gcaagcacgg
     1981 gcgaagagga tgatcgatct ggttggatta gacaagaatc ctggcaatga cccggccgcg
     2041 agtgaccgcc gctggacgaa tcgtctagaa gcctggggta tggcaaaacg tgctaggcag
     2101 catgtgcaga aatttcctgt tcccgaaatg cgcgaccagg ttgttgatac cgctctcgca
     2161 aagggcttcc ggtcaatctg gatgaccgtt ttccaagatg atcccattat gcgggttagt
     2221 ttcatcaata gcttcaaggg taccagtgcc acctcatttg gtcctggcgc caacccgata
     2281 catcgtccca atggcgacct ctaaagttct tactctttcc caatagggat aaattcggca
     2341 aaatatttgc ctccaaattg aaatgtgtcc aacagatagt aatcgtccca aaattggttg
     2401 gctttgaaaa aaacagtaag ctccgttatg atgggagttg tcacaccacc gtcaaacgga
     2461 gcttgctatg cccattgtgg tcttgaaact accggaagtc aagagcgcag ctgaaggaag
     2521 gccgcaacgc tgcccgagtt gcaagggacc tgccccctgt ttttgcacca ctcactgagt
     2581 tcgtccaagt ggctgaaaag gccgatccat caccactttg ggagccgggg gcctgtatcc
     2641 ctgagcgctg tgcggcttga tcgtattgta ctcgagtcgc caacgttcat tgagtatctc
     2701 cgctggtgtg aattgggtca gacaataatt ggaaatctcg ggagcggagc caggaaacta
     2761 gaggaataat atcgattccg gaaatcttaa aagcagtgcc caaaatcact tgacacatgc
     2821 ttccagaatt tcaagcctag cagaggccgg atccaaacgg caccgagccc cgacgggaag
     2881 ggtcgtgttt gggcagtgat ggccgaagtc gtccgtctta acgattggga agtcatattc
     2941 agcggtaact tccaagaaaa tgtcttcggc cttgtaaccg tctttttcgt ttgcgaaacc
     3001 gatcagtacc ccccttactt tttcaaatat ccccgcctgt ttgaattggg tcaggcgatt
     3061 ataaaaggat ccgggggcga agccggggat ctcgataaac aaaatcgagt ctgacatatc
     3121 tggaaaatac ggcgtaccta aaagccaaga cattacccaa tcgttgcccc cgagcagctt
     3181 accctctgcg ctcttttccc cccttacagt cctccattcg ctatgtttat taaccgggcc
     3241 gatcttgcct gtcaccaatc gatccagaaa ctcctgacgg tcatactcct gcggatccat
     3301 tccaaaatac caaatcacca tattaccgtg aaaggtcaca agcccagtct gggaatagat
     3361 tcccagatga agggtgctga catcgctgaa acccagcaag atcttaggat tacgccgaat
     3421 aagatcataa tccagataag gtaatatgga atttgcattt atgccgccgt gtgacgatat
     3481 cagcgcatgg atatcgggat tggcgaacat atggttgatg tcatctgcac gttcctgggg
     3541 agttccagct ccgaaagccc ctcttttcaa tgcgttttca ccgagcacaa ccttaaaacc
     3601 gagtttttcc aaaaagcgtg ttcctttttc cagctcttca tatggcgact ccgatgggga
     3661 atcgtcaatt tcaccagaag gcgtgaaaat accgatcgtg tcaccgatgt gaagacgagg
     3721 ggctaagagt gtcatagttc attgctcctg atcatttatt ccggaaaatg caaaaaccaa
     3781 ataaagtttg ttcacctcta tccggtaaat ccgcttcttg cggtctcttt taaaacatct
     3841 tcggtatttg tgaatggcct tcattccatc cacttgcttt gaaagcgcat acgcttgaaa
     3901 ttggagttac tgctcaataa ctgtatgtcg tcaaagtaaa acgcacatga cctgccacca
     3961 gtttttgcac cactcaccat gtttgtccga gtggctgaaa cggccgctcc atcaccactt
     4021 tgggagccgg gggcctgtat ccctgagcgc tgtgcggcct gatggtattg tactctactc
     4081 gccaacgttc aatcagtatt tctgtctcct tcactgaaca gaaaatttca cgattcaaca
     4141 gctcatctcg cgttttccca ttgaagattg gttcaaccaa ctttagaaaa ttttaagaaa
     4201 cactcgcaaa aattatgatt taatatatac aaatttaccc atcttaatct tattggagaa
     4261 cgtcaatgaa acacacagaa gttaaaagca caatcacttg cgatatggaa ggccgtattg
     4321 aaacgtataa caagggcgct gaagaaatat ttggctacag tgccgaagaa gtaataggcc
     4381 ataagcgagt atcactattc tcacctggac tgatcgtctt ggaacatgta ccaacctggc
     4441 tgaagactgc cagcgagact ggagaatata aaggccaaac cgtgtttgtt cgaaaagacg
     4501 gttcaacttt caacgctgaa attcgcatta cacccacttt tcgcgatggt aaacaggtcg
     4561 gatactgcgg ggtcaccaaa cctctggatc catcccagag ggcaaatgca atgccgaaaa
     4621 tcagtatgtt gaccaaagtt ttcgctggca tggtaatcac gcgaatgcca tttctgacag
     4681 ccagccttat cccagtctta atcggtgcaa cctgggcagt ggttgcgcgc tcggcatcgc
     4741 ctttcccctg gttggtattc atccttacat tgcttggtgc cgcagccctg catgttgctg
     4801 ctaacacatt caacgattac tttgattgga caagtggtac agataaagtc aataatgact
     4861 actttttacc tttctctgga ggaagtcgct caatggagct tggcttaatt tctgaaaaag
     4921 gtgtgttgaa tgtgggcctg gttgctttag gtttggcctt tgggtttggc ttgaccgtga
     4981 tgttcttgag ccaacaacca ttgctaatcc tgtttggact tgcaggggca ttctcagctt
     5041 atttctacac ggcaccacca ttgcggctgg ttgctcgcaa agggttgggg gaattattaa
     5101 ttggattgaa ttttggtccg ttgatgactg ctggcaccgt gctagcactg acaggtcgcg
     5161 tggcgtggga agattttgta attggtttac cagttgggtt gctaactaca gccattctat
     5221 ggatcaatca atttccggat gagattgccg atcgcaaaag tggtaaacac aatctagtgg
     5281 tagtgctggg tacagaacgt gcccgctggg gctatttaac cctgttggtt gctgcttttg
     5341 gcattagtat aatttgtaca ctggcaggac tatttccaat cggcacgctg gcgatgcttg
     5401 ggggagttcc gctagcagtc tcagccagcc gtatcctctt ccagcattac aaggatcgag
     5461 aacttgtatc tgccaatgcc aaaacaatta accttcaatt gatcgcgggg ttgcttaccg
     5521 cagcgggcat tttgatcagc cctctgatag gatagctgcg atctcattct taatgttgta
     5581 acctcgtgaa ctatttcttc tcttgatttt tgtattccgg atggttcgga ataggtccgc
     5641 tatgggctac tcaaaaagac acctccaagg tgtctttttg tttgtattta tgggaaatta
     5701 gcaacggaac ctatgatccc cagatctcac ttgccctaaa ttaaaccgtg cagttagttc
     5761 aatcgtgttt ggccaatgta aatttgatga aggcgatgtt agcacaggcg gcaatgttgc
     5821 ggataaatcg cgcatgttag ataggttacc ctttcaccat tttcgatcgt tattccacga
     5881 ccagcagcaa gtctagaatt cggcgtttcc cttcatatat ggtgtttagc gatctccaat
     5941 ggcaaggata aactccagaa attgtattcg ttgagagaag agtaatggtg accaacgcaa
     6001 cattccggtt cagcagacgg cttgctgccg tcgctgaacc ggttgttagc tgaccgttat
     6061 ttcgccgccc tgtcagaagc ctttgaagtt gtttatgcta agttcaggcg aggtcaaatc
     6121 ggtc
//
LOCUS       DBNGSX010000301         3725 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1548144, whole genome shotgun sequence.
ACCESSION   DBNGSX010000301 DBNGSX010000000
VERSION     DBNGSX010000301.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1548144"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..156
                     /locus_tag="AC16CB_09095"
     CDS             <1..156
                     /locus_tag="AC16CB_09095"
                     /inference="COORDINATES: protein motif:HMM:NF014009.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HIR3342663.1"
                     /translation="HVRAALLKASLTVPFVRARLTLGTWQQIIYVDFDNRSRRRELVV
                     QIIGEVG"
     gene            complement(195..1034)
                     /locus_tag="AC16CB_09100"
     CDS             complement(195..1034)
                     /locus_tag="AC16CB_09100"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF021507.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HIR3342664.1"
                     /translation="MTFTILGGLVGFNWLMFIPRSQPKPANQALFQGITYVREVRNSS
                     RPMVAHVVTVNLRAPGISLLVTPGDPKAERPLKARTTSKFLDQFGVQIAINGDGFTPW
                     YSNNILDYYPRSGDAVEPIGFAASEGKVYSDNLHEQPVLYFTRNNQARFGTPPGKIIN
                     AISGNLMLVQDGKVLPSLEGTREPRTAIGLDKRGRSLIIVVVDGRQPGYSEGATLEEL
                     ASILVAHGAHTAMNLDGGGSSTLVIEQGGKATLLNSPINHGIPGWERPVGNHLGVFAE
                     QTK"
     gene            complement(1085..1900)
                     /locus_tag="AC16CB_09105"
     CDS             complement(1085..1900)
                     /locus_tag="AC16CB_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HIR3342665.1"
                     /translation="MSKVRADILLVNRGLAESRSQAQRLVMAGQVRADGQVIPKPSTP
                     VADDAVLVVEQGPPFVSRGGEKLHAGLHAFPLDPASMICADVGASTGGFTDCLLQHGA
                     AKVYAIDVGKGILHWDLRQDPRVIVMESCNARYLENLPEAVDLVTIDVSFISLQVLLP
                     VVKGWFRPPSSTQFQTGEAKPVGQVIALIKPQFEAGRREVSRGDGVIRDPSVHRRVLL
                     DVLSNAQEHGYYPRDLLRSPLLGPKGNAEFLAWFTYPASEQDNIEELINNLFL"
     gene            1968..3344
                     /locus_tag="AC16CB_09110"
     CDS             1968..3344
                     /locus_tag="AC16CB_09110"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HIR3342666.1"
                     /translation="MTNFRAVAIDYAHLNHQRFLDALKEFVSIPSVSTDPERSNDMQE
                     AAQWVADRLRDLDCLNVQLFPTAGYPVVYGELMKAGPAAKTILVYGHYDVQPAEPLEL
                     WESDAFEPEVRGDNLYGRGASDMKGQVMATLAAVEAVSHNGGMPVNLKFLIEGEEEIG
                     SPNLEEFLEAHKDMLSCDFAINPDTGMLAADLPTITYALRGLAYFELRVYGPSHDLHS
                     GLYGGVVHNPAQVLCDLISGMHDSQGRVTLPGFYDNVRPIDQDEREELSRLPTTDAFY
                     REETGVPVLWGEVGYTPVERVGARPTLEVNGLYSGFTGEGSKTVLPAWAMAKISCRLV
                     PDQNPDVVYRQLKAYLENHAPETVRYEVKQLVNGPASISDRQSDAVQTVIKALETVWG
                     KRPAFRREGGSVPVVAHFQTYLGIETANFGFALPGDNAHGPNEKLHLPTWQRGIDALV
                     HFFFNIGE"
     gene            3355..>3725
                     /locus_tag="AC16CB_09115"
     CDS             3355..>3725
                     /locus_tag="AC16CB_09115"
                     /inference="COORDINATES: protein motif:HMM:NF018798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HIR3342667.1"
                     /translation="MSEPKLITYGGQAVLEGVMMRGGKAVAIAMRAPDQQIVIHTEKL
                     GGIYRSRIVKIPFLRGLVVLWDALGLGMRALTISANTQTGEDEQLEGPVLYLTLGISL
                     ALGIGLFFLLPAAAGQMAERYL"
BASE COUNT          868 a          949 c         1009 g          899 t
ORIGIN      
        1 catgtacgtg cagccctgct caaggcctca ctcacggtac cctttgtgcg cgctcgcctt
       61 accctgggca cctggcagca aatcatctat gtcgatttcg acaatcgttc caggcgcagg
      121 gaactagtag tccaaattat tggggaggtt ggctaacctc taaggtcaaa cggtgaggtt
      181 gatctcgcgc caccctattt tgtctgctcc gcgaaaacac ccaagtggtt acccaccggt
      241 cgttcccatc ccggaatgcc gtgattgatt ggcgagttga gcaatgtagc tttccccccc
      301 tgctctatta ccagggtgct agagccgccg ccgtccaggt tcatggctgt gtgcgcgcca
      361 tgggcaacca agatacttgc cagttcttct aaagttgcac cttcactgta gcctggttgg
      421 cgaccatcca caaccacaat aatcaaagac ctgccgcgct tatccagacc gatggctgtg
      481 cgcggttccc tagtaccctc caggctgggt aaaactttgc catcctgcac caacatgaga
      541 ttgccggaga tggcgtttat gatcttacct ggcggcgtac caaagcgggc ttgattgttg
      601 cgagtgaaat acagcaccgg ctgctcatgt aagttatcag aatatacctt tccttcagag
      661 gcagcaaatc caatcggctc aaccgcatcc cccgagcggg ggtaatagtc caggatatta
      721 ttagagtacc agggtgtaaa tccatcccca tttatggcga tttgaacgcc aaactgatcc
      781 aggaattttg atgtggtgcg ggctttcagt gggcgctcag cctttggatc accgggggtg
      841 accagcaggc taatacccgg ggcgcgcaaa ttgactgtga ctacatgagc aaccatcggg
      901 cgggatgagt tccgcacttc acgaacatag gtaattcctt gaaatagcgc ctggttggct
      961 ggcttaggct gagatcgggg gatgaacata agccaattaa agcccaccag cccgcccaga
     1021 atagtgaatg tcatcagaat acaaagtgga atgaagacca gtaaaaggca gcttcttttt
     1081 tgtttcataa aaatagattg ttgattaact cctctatatt gtcctgctcg ctggctggat
     1141 aggtaaacca ggccaaaaac tcggcattcc ccttcgggcc caacagtggc gagcgcagca
     1201 aatcgcgcgg atagtagcca tgttcttggg cgttagagag aacatccagc agcacgcggc
     1261 ggtgcacaga cgggtcacgg attacgccgt caccgcggga tacctccctg cgaccggcct
     1321 caaattgcgg tttgatcagg gcgatgacct gaccaactgg ttttgcctca cccgtctgaa
     1381 attgggtgga tgatggagga cgaaaccagc ctttgacgac gggcaagagc acttgcagcg
     1441 agatgaacga aacgtcaatt gtaaccaaat ctacagcttc gggcaaattt tcaagatagc
     1501 gggcattgca gctttccatc acgatcaccc gagggtcttg gcggagatcc caatgcagga
     1561 tacccttgcc gacatcaatc gcatagactt ttgctgcccc gtgctgcagc aggcaatcgg
     1621 tgaacccacc tgtcgaagcg ccgacatcgg cgcagatcat gctggcagga tccaggggga
     1681 aggcatgcag ccctgcatgc aatttttcgc caccacgcga tacgaaagga ggtccctgct
     1741 ctacaaccag cacagcatcg tcagcgactg gggttgaggg tttaggtatc acttgcccat
     1801 cagcccgaac ctgccctgcc attaccaggc gttgtgcctg gctccggctc tcggctaacc
     1861 cgcgattgac tagcaatatg tccgcgcgta ctttcgacat atcacaattg taactgaaga
     1921 tgccggggta taattgggcg tgcattttaa ttatctagga gacattcatg accaatttcc
     1981 gtgcggttgc gatagattac gctcacctga atcaccagcg ttttcttgat gcgttgaaag
     2041 agtttgttag cataccgtct gtatctaccg atcctgaacg aagcaatgac atgcaagaag
     2101 ctgcccaatg ggtagccgat cgattgcgtg acttagattg cctgaatgtg caattgtttc
     2161 caactgccgg gtatccggtg gtttacggtg agttgatgaa ggcggggcca gcggccaaaa
     2221 ccatcctggt ctatggacat tacgatgtcc aacccgccga gccattagaa ttgtgggaaa
     2281 gcgatgcatt tgagccagag gtgcgtggtg ataatctgta cggccgcggc gccagcgata
     2341 tgaaaggtca ggtcatggcg actcttgccg ccgtcgaagc agtgagtcat aatgggggaa
     2401 tgcccgtgaa tttgaaattt ctcatcgaag gtgaggaaga gatcggctca ccgaacttag
     2461 aggaatttct cgaggcgcat aaagatatgc tgtcttgtga ttttgccatt aatccggata
     2521 ccggcatgct ggcagctgac cttccgacaa ttacgtatgc attgcgtggc ttggcatact
     2581 ttgagttacg cgtatatggc cccagtcacg acctgcattc cggcctgtat ggtggcgtgg
     2641 tgcataaccc tgcccaggtg ttatgcgatc tgatttctgg gatgcatgac tcgcaagggc
     2701 gtgtaaccct gccaggtttt tacgataatg ttcgtcctat tgaccaggat gagcgagagg
     2761 agctatcccg cttacctact actgatgcat tttatcgtga agagactggc gtcccagttc
     2821 tttggggtga ggtgggttat acgcccgtag aacgcgttgg ggcgcgtcca accctggaag
     2881 tcaacggtct gtattcgggt tttaccggtg agggcagcaa aacagtgctt ccggcctggg
     2941 ccatggcaaa aatctcctgc cgcttggttc ctgatcaaaa ccccgacgtt gtttacaggc
     3001 agttgaaagc atatctggaa aatcacgcac cagagactgt gagatacgaa gtaaagcaac
     3061 tagttaacgg gcctgcttcc atctcggacc gacaatctga tgcggtgcaa actgtaatta
     3121 aagccctgga aactgtttgg ggcaagcgtc cagccttccg acgggaaggg ggcagcgtgc
     3181 cagttgttgc acactttcaa acttacctgg ggatcgaaac ggcaaatttt ggctttgccc
     3241 tacctgggga taatgcccat ggccctaacg aaaagctgca cctgccgact tggcagcgcg
     3301 gcatcgatgc cctggtgcac ttctttttca atatcggtga atgaggttgt aaatatgtca
     3361 gaaccaaaat taattactta tggcggtcag gcggttctag aaggggtgat gatgcgtggc
     3421 gggaaggcag tagctattgc catgcgtgct ccagaccagc agatcgttat acacactgaa
     3481 aagcttggtg gtatctaccg cagtcgtatt gtaaagattc ccttcctgcg gggtctggtc
     3541 gtgttgtggg atgctctagg tcttggtatg cgcgccctaa ctatttcggc gaatacccaa
     3601 actggagaag atgagcaatt agaaggtccc gtcctgtacc ttacgcttgg gatttcgctc
     3661 gccttgggaa ttggtctgtt cttcctgttg cctgcggctg ccgggcagat ggccgaacgg
     3721 tatct
//
LOCUS       DBNGSX010000302        13666 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_440595, whole genome shotgun sequence.
ACCESSION   DBNGSX010000302 DBNGSX010000000
VERSION     DBNGSX010000302.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13666)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13666)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13666
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_440595"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..99
                     /locus_tag="AC16CB_09120"
     CDS             <1..99
                     /locus_tag="AC16CB_09120"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR3342668.1"
                     /translation="YPLEQAAEAHRYVEEGHKRGHIVITVKHNGKT"
     gene            135..1136
                     /locus_tag="AC16CB_09125"
     CDS             135..1136
                     /locus_tag="AC16CB_09125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016103795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIR3342669.1"
                     /translation="MKAIVWTKYGPPDVLELREVEKPTPKDNEVLIKIHATTVTAGDC
                     ELRSLKLPIYISLPLRIWLGFREPRSNTIPGTELAGKIEAVGKYIKKFKVGDQVFGSA
                     GMGFGANAEYLCLPEEPGEMEGGVAIKPTNMTYEEAATVPFGGRDALHFLRKGNIQSG
                     QKILINGAGGSIGTFAVQLAKYFGAEVTAVDSTAKLDMLTSIGADHVIDYTLEDFTKR
                     GEIYDVIFDVVGKISFLRSNKALKQNGTYLLANPGSQVVQGLWTSLTSSKKVIMQTAS
                     GTIADLIFLKELIEAGKIKTVIDRRYPLEQTAEAHRYVETGQKQGNVVITVDHDDKT"
     gene            1189..1347
                     /locus_tag="AC16CB_09130"
     CDS             1189..1347
                     /locus_tag="AC16CB_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342670.1"
                     /translation="MSKAEFLTVRWNNLLTLGLGLPALIYVIMAFSTSFWTGRGGLIG
                     LSIIGVLY"
     gene            1898..3079
                     /locus_tag="AC16CB_09135"
     CDS             1898..3079
                     /locus_tag="AC16CB_09135"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR3342671.1"
                     /translation="MTDEKTITTDSSKKTSTNRKRIHWMDNLRTIMILLVVLYHVGGV
                     YEGAGLWGWFWIVDDPDTISWVGIVGIVIDIIVMPTIFFISGYLAPASLKNKTGLEFL
                     KGKFKRLMVPWVIAVLTLIPLYKVIFLYSRGLPQEHWTTYSHITNPNSQNWLWFLPVL
                     FTFNMLYLLFTKAKIRIPNISLKGAVLGTFLIGFVYSLSIGGILGFRSWTLTPLIDFE
                     NERLLLYFMAFLLGVLCFRQNVFAEKPKSKTLYTVVNSIAWIPITVHIFARLIPFFVP
                     EGFLISPLVDRLIWWLSFYLSLLCLLYLLVETFWRYFDKTGSIWSELNRNSYSVYIIH
                     VIVIGIFGTLLLNLNLPALVKYPVLIVLTYLGSNWLISSYRKVSAEFKKLFIEWRKLA
                     L"
     gene            3253..3504
                     /locus_tag="AC16CB_09140"
     CDS             3253..3504
                     /locus_tag="AC16CB_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020516417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342672.1"
                     /translation="MNKVKQKLTLDRPATYQIRVPGHLDKSWSEWTREMTISTESDED
                     NLPVTTLMGTFDQAALHGLLRHLYSLGIPLISVNYVERV"
     gene            3607..5121
                     /locus_tag="AC16CB_09145"
     CDS             3607..5121
                     /locus_tag="AC16CB_09145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3342673.1"
                     /translation="MTEHSIIVIGAGIAGLSAGCYGQMNGYKTRIFEMHNELGGCCTS
                     WKRNGYTIDGCFHYLTGSSPGQSFYHIWEELGALEGRGIVNHEEYARIEGNDGKVFIV
                     YSNIDRLERHMTELAPEDTEVIKEFTDGIRTMIHFPMPVEKPQELYSPIDGFRMMFKM
                     FPFLSFYRKFGKVTIEDYAKRFKNPFLRQAFLYIVNLQNSTDFPMLGIMQVLAWMNQK
                     TAGFPVGGSLEFAQSIGRRYIDLGGEIQYASRVVKILVKNNRAVGVRLADGSEHHSDI
                     VISAADGHRAIFEMLDGEYINDKIKGYYEELPITPPIIHIGLGVARSFEELPHSVTGT
                     DLVLDEPITIGGQERSRMCVQIYNFDPSLAPAGKTVVKVYYASDYEYWKKLIRDSARY
                     EAEKELIADQVIAVLDQRFPGLASQVEMSDVATPMTFEHYTGNWQGTFMGWQTSTKTL
                     IMRMSKTLPGLKNFYMAGMWVEPGGGVPTAAMSGRNVIQIICKLDKRPFKTKLP"
     gene            5296..6192
                     /locus_tag="AC16CB_09150"
     CDS             5296..6192
                     /locus_tag="AC16CB_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HIR3342674.1"
                     /translation="MKQLDKRQITGWIGVGFSIVITCTWAFWGIIETFHEGWYYESFL
                     SNVGLMLAQYLSPMLIFMGVTLISISWPRFGGGLHLILALLVAWFFQVFTNAAIFMLI
                     LPLIGIGALYWFGRPEPRKIALLLAAGLPMLILVISGVEPVLRVSQRFDDGNLQAQLV
                     YGNGVDLVWAPDGPGWPRTGVNWHEALQVCQHLSEDGLTLSLESLDIWRLPTVDEAVR
                     SMARHGQNSGGVWDAEIAEATYETTPDKESPLWNIHSQVIYWWTATTVDEEQAFIIVY
                     DGKVWPRSKQLSLAYLGFRCVK"
     gene            6486..7199
                     /locus_tag="AC16CB_09155"
     CDS             6486..7199
                     /locus_tag="AC16CB_09155"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR3342675.1"
                     /translation="MDPITLVIIVIASYLLGAISFARLIPRIVDPKADLSDVSLPVEN
                     SEEKMPMLAMGANTASIKYGPKVGCSIGLLDIFKVFLPTLLVRVLYPDASYYLAAAIA
                     GFIGHCWPVYYQFKGGRGISAFYGALFAIDPLGAVVVASTAMFIGMVLLKELLVAYTG
                     GVLLTVVWFAITRGWDFHLAYALVINVLFVVAMIPELKMIREIRQKYGREDMRNSMST
                     FPMGQAMLKLMDALKIQRK"
     gene            7216..9606
                     /locus_tag="AC16CB_09160"
     CDS             7216..9606
                     /locus_tag="AC16CB_09160"
                     /inference="COORDINATES: protein motif:HMM:NF013491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR3342676.1"
                     /translation="MEYLVSLHTNKSPDNIGNKALNLHRLWRKKFNIPRTYICTWNAF
                     LDFQSLGSVAVDALSKELEQIIKPERSYAVRSSANFEDNNQRSFAGQFTSLLNVRGVD
                     HVAQAVQSIWEATESEGVQVYLKQSMQPKDRLLMAVIIQEMIEPVISGVSFSRNPITG
                     AEEIIVEAVRGEGSQLMQTGITPMRWVSRWDIMLDKPDGESQLNRLIEQIVKETNQIA
                     ILLRRDIDLEWVYDGRSLYWVQLRDITSLQEGDVYNNQIAREMTPGLIKPMVWSISIP
                     IHTGQWVRILNELVSNTQIEPARLVKAFHYRAYYNMGQFGRVFASLGMPRDSLERMMG
                     VLPPGVSRPKFMPGLSFLPKAPQVIRFILDKLRFGHRAERDYPILRQEARAFSGPLTA
                     YDHPEDLLERVDQIVCLQEKISYNTIMSILLMQIYNGLLRSHLKNRGVDPSQFDLTDR
                     WESMQAFDPNLHLANLHVQYQTLEPQLQHELKQGNLQALELEASLSDFRRDFYQFLDR
                     FGHLSDSTVDFTSIPWRESPDLILRLVCDFSPRDKQSVSKIGLQDLKTKRLSFFYLRF
                     IYQRARQFSLFREMYSSLYTFTLMLLRDHFHALGTILTVSGLLPSWEDIFLLYMEELR
                     SWVEGDADGKDFAALVTQRNDEMERSRHAQVPDVIRGDTIPPLIPANAQKLQGIPTSG
                     GYYAGRVKVIHGLNEFHKLEPGDVLVIPYSDVGWTPLFAHAGAVIAESGGILSHSSII
                     AREYNIPAVVSVPTAMNLPDGSLVEVDGYKGLVYLNDGSQPASAVQEGATDASSAN"
     gene            9587..10336
                     /locus_tag="AC16CB_09165"
     CDS             9587..10336
                     /locus_tag="AC16CB_09165"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3342677.1"
                     /translation="MPVLQTNDLKKQYQMGTKRVDALAGVDFLVEKGEFVAIMGPSGS
                     GKSTLLHLVGGLDQPSGGEVYLAGARLSQLKDKKVTLLRRRNIGFVFQFFNLLPTLTA
                     EENITLPLLIDGKNLKGFQQRINTLLQLTGLSDRRWHKPEQLSGGEQQRVALARALVT
                     EPAIVLADEPTGNLDSKTGTAIMELLRKSCDELEQTIVVVTHDPRAAMYADRVVFLRD
                     GHIDGELRFLPLTPLTDKLHQIIERMTKLGE"
     gene            10348..12921
                     /locus_tag="AC16CB_09170"
     CDS             10348..12921
                     /locus_tag="AC16CB_09170"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3342678.1"
                     /translation="MNLLRLLSLRSLTTRPMRVLLSTFGIVLGVAAIIGIGVTNQTAL
                     NSVTQLFENTSGKADLIITSREADGRGIPETTAQRMEKFSGVFAALPSLQVQTVLANN
                     KQSDDISLGFFGTSLGGLLLYGIDPLRDSQARDYSIVAGRFLSPDLLADEVVLVRSYA
                     DQNELEIGKYIQFVAAGQVEKLRIVGLIEREGPGQLNNGAFGVMPLGTAQRLFDRSDE
                     IDQVDLVMSPGLSSDQLESERLRLLSYLGDSYSVIYPASQGKRMTQMLSNYQIGLNFL
                     SAMALFVGAFLIYNAFSMTVVERTREFGMLRTLGMTRLQVAFQVLVEAFSMGAVGSLL
                     GICLGLLMARGLSQLMAVIMAQEMPQAQAPQDLMVIAFTIGVLVAVAAALLPALRAGR
                     VSPLEALRVRVLVNQGWLIRYGWTAGIALLVAAGWGLVFNPFPNDPQFRFGSLMVFAL
                     FLGGTLVIPISFSVWERTARPLMIVLFGRVGQLGSRNIQRSRLRTTLTVAALMIGVAM
                     IVIVWVMTDSFKGDLDEWLKGYIGGDIYVTSLVPIRENIVRRIEVVPGVEAVAPVRYF
                     EIEWRRPGNDWETVNFMAFSPASYTHVTSFVFSQSDSESINNILRGGHVFISSVIAEK
                     HGLAPGDRLLIKTRLGETPFTVAAVTVDFSNQGMVINGSWNDMEHYFRLRGANTLLIK
                     VTPGTSPDAVGEEIDSRYGKGENLVIISNETLLGRISTLMQQAFRMFDVLALIAMLVG
                     FMGITNTLTMNVMERTQEIGMLRAVGMTRGQVVRMVLSESALMGLIGGLMGLVFGAVL
                     SRLFLSAMASMSGYRLTYVLPIENILEALLIAFLTSQLAAFLPTLRASRTRILEAIQY
                     E"
     gene            complement(13084..>13666)
                     /locus_tag="AC16CB_09175"
     CDS             complement(13084..>13666)
                     /locus_tag="AC16CB_09175"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HIR3342679.1"
                     /translation="FHVLEDEVITASYATAVYLRSQNPRSCWVMVDREGIQEFKAFVQ
                     DTDNPEYIVIGDNRSQFNFDHLNKVLRHLVKGAKLIAMQSELIDTSMGDIELNVGSWV
                     GMLERAAGVRAISIGKPNPYVFELALDSMHLGKNDVIMVGDRITTDIQGANYFGLRSV
                     LIKTGEFDERDLVSEIVPTYTFETIGELLELFS"
BASE COUNT         3470 a         3174 c         3404 g         3618 t
ORIGIN      
        1 tatccgttag aacaagctgc cgaagcccac aggtatgttg aagaaggaca caaaagggga
       61 catatcgtca taactgtgaa acataacggc aagacctgac aaagcgtcgc acctggtcat
      121 taggaggagc acagatgaaa gcgatcgtat ggacgaaata cggaccaccg gatgtgcttg
      181 aattaagaga ggtggaaaaa cccacgccca aggacaatga agtacttatc aaaattcatg
      241 cgacaacagt aacagcaggg gactgtgaac tccgaagtct taaactccca atctacatca
      301 gtctcccttt gcggatatgg ctaggtttca gagaaccaag aagtaatacc ataccaggga
      361 ctgagctagc cggaaaaatt gaagccgtag gtaaatatat aaagaaattt aaagtgggtg
      421 accaggtatt tggatctgcc gggatgggtt ttggcgcaaa tgccgagtac ttatgcctgc
      481 ctgaagaacc tggtgagatg gaaggggggg ttgccataaa accgaccaac atgacctatg
      541 aagaagccgc caccgttcct tttgggggac gagatgcatt gcattttctt agaaaaggga
      601 atatccaaag tgggcaaaag attctgatta acggtgcagg tggaagcata ggtacttttg
      661 cagttcagct tgccaagtac tttggggctg aagtgactgc tgttgacagc acggcgaaat
      721 tggacatgtt gacctcgatc ggtgcagatc acgtcatcga ctacacacta gaagatttca
      781 caaaaagggg tgagatctat gatgttattt ttgatgtggt cggtaagatt tcgtttttac
      841 gtagcaataa agcgctaaaa caaaatggaa cctatttatt agccaaccct gggtcgcagg
      901 tggttcaagg gctatggacc agtttgacaa gcagtaaaaa agtaattatg cagacagcaa
      961 gtggaacaat cgcagattta attttcctga aagaacttat tgaggcgggg aagataaaaa
     1021 cggtcatcga tcggcgctat ccattagaac agactgccga agctcaccgt tatgtcgaga
     1081 cagggcaaaa acagggaaat gtagtcatca cagtggacca tgacgacaaa acctaggcca
     1141 aaattaggcg tcattacctg aaagacggca tactgaaagg atgaaacgat gtcaaaggca
     1201 gaattcttaa ccgttcgttg gaataatttg ctcacgctgg gtttgggact tcccgccctt
     1261 atctacgtga tcatggcttt ttccacttcc ttttggacgg gaaggggtgg gttgatcggc
     1321 ttatcgatta tcggggtgct ttattgactg gtacttgaag gtcatacctc catgcggttc
     1381 gcatggcttc gagaaaactc ggcgaactac gtctccgtca aacagggatt cactcaccca
     1441 cttgcgttaa ttcggaggat ttataacgct gctttctgga tttttttgct gccctttttc
     1501 acgctcatcg attatggcac aggatttcta acctttaccg tcattattgc tatcaggctg
     1561 ggtttaaatc tatatacaaa caacttcctc gatctcacgc cagcgcagta cgatagtttt
     1621 cccttcagaa ttccatgaat attcgaggga cctaacattc acctactttg attgagctgt
     1681 acggcgtcag ccattaaacc acctgctaca aaatacgggc cgctatttgc tggcccgtgt
     1741 ttttttacct ctcgaatcca caattaaccc cctgaataac actttcgggg tatgacacta
     1801 atactgccaa gcttgtatgc taaaactgta aggattattt tttaaacaag gctggttggc
     1861 gcctccatct ttttcaaatg atataaggaa tattctcatg actgatgaaa aaacaatcac
     1921 cacagattct tcaaagaaaa cctcgacaaa taggaagcgg attcactgga tggataacct
     1981 cagaaccatt atgatcctct tagtggtcct ataccatgtg ggaggcgttt atgaaggtgc
     2041 cggtctttgg ggttggttct ggattgtgga tgatccagat accatatctt gggttggcat
     2101 cgtaggtatc gtaatcgata ttatcgtgat gcccaccata ttctttattt caggttatct
     2161 ggctccagct tccttgaaga acaagactgg attggaattt ctcaaaggga aattcaaacg
     2221 gctgatggtc ccctgggtga tcgccgtgct tacgttgatc cccctctaca aagtcatatt
     2281 tctatattct cgaggattac ctcaagagca ctggactacc tactctcaca taaccaaccc
     2341 aaacagccaa aattggttat ggttcctgcc tgtgctgttt accttcaata tgctctatct
     2401 gctttttaca aaagcaaaga tcaggatccc gaacatatcg ctaaagggcg ctgttttggg
     2461 cacctttctg attggatttg tatatagctt gagcatcggt gggattctcg gttttcggag
     2521 ctggacctta accccgttga tcgattttga aaatgaaaga ctgctcctgt acttcatggc
     2581 ttttttgtta ggtgtgctat gttttcggca gaacgtattt gcggaaaaac ccaaaagcaa
     2641 aacgctgtat accgttgtaa attcgattgc ctggattccg atcactgtcc acatctttgc
     2701 acggctgatt cccttttttg tgccagaagg ttttttgatt tctccgcttg tggataggct
     2761 tatttggtgg cttagttttt acctttcgct gctctgtttg ctgtatctat tggttgaaac
     2821 attctggagg tatttcgata aaaccggaag catctggagt gaactgaacc gcaattcata
     2881 cagtgtttac atcatccatg tgatcgttat cggcatattc ggtacgctct tactgaacct
     2941 gaacctgcct gctttagtga aatatcccgt tttgattgtc ttaacctatc tcggcagtaa
     3001 ttggctcatc tcctcttatc gaaaagtttc tgctgaattc aaaaaactgt ttattgaatg
     3061 gaggaaatta gctctataaa tacaatactc tatcctatca tcgtcaatgc cttgttttgc
     3121 agactctcgg catgacactt ccgcagcatc tggatgtcca tcatcgtccg ctctggtcaa
     3181 agcgtgtttt ttgcattcct aatcctcgat tttgtgctgg ggtggcgaag tggcttgaag
     3241 aggtaatcaa ccatgaataa agtcaaacaa aaacttactt tagatcgccc ggcaacctac
     3301 cagattagag tgccggggca cctcgataag agctggtcgg agtggacaag agagatgact
     3361 atttcaaccg aaagcgatga agataatcta ccggtcacga ccctgatggg aacctttgac
     3421 caggcagcgc tgcatggtct gttgcgtcac ctctattctc tcggtatccc tctgatatca
     3481 gtcaattatg ttgagcgcgt ataaaacctc caacgctcac aaatttggag catcaacaca
     3541 ggcttcggtg agattggtaa tacactgaaa acatggatga agaactttgc tgaaggaggt
     3601 ttaaccatga cagagcactc aataattgtt atcggtgctg gcattgctgg cctctccgct
     3661 ggttgctatg gtcagatgaa cggctacaaa actcgcattt tcgagatgca caacgaactt
     3721 ggcggttgtt gtacatcatg gaaacgcaat ggctatacga ttgacggctg ctttcactat
     3781 ttgacaggat caagtccggg gcaaagcttc taccatattt gggaagaatt aggagcatta
     3841 gaaggtcggg gtatcgttaa tcatgaagaa tacgctcgca ttgaaggaaa cgatggtaag
     3901 gtcttcatcg tctactctaa catagatcgc ttggagcgac atatgacaga gcttgcaccg
     3961 gaggatacgg aggtcatcaa agagtttacc gatggcatcc gtacgatgat tcacttcccc
     4021 atgcccgtag aaaagccaca agagctttac agcccaattg acgggtttag gatgatgttt
     4081 aagatgttcc ccttcttgag cttttataga aaatttggca aggtaacaat cgaggactat
     4141 gccaaacgct tcaagaatcc attcctccgt caggcttttt tatatattgt aaatctccaa
     4201 aacagcactg acttccccat gttagggata atgcaagtgc ttgcttggat gaaccagaag
     4261 acagctggat ttccggttgg tggctccttg gagtttgccc aatctatcgg acgacgctac
     4321 attgatttag gaggcgagat tcaatacgct tcgcgggtag ttaaaatctt ggtaaaaaat
     4381 aatcgggcag ttggtgtgag gctggctgat ggcagtgagc accacagtga tatagtcatt
     4441 tccgccgctg atggtcaccg tgcgatattt gaaatgctag atggggaata tatcaatgac
     4501 aagatcaagg gctactatga ggaacttccc atcacccctc ccataatcca cattggactg
     4561 ggcgttgcgc gatcgtttga agagctgccg cacagcgtga caggtacaga cctcgttctc
     4621 gatgagccaa ttaccattgg cgggcaggaa cgcagtcgaa tgtgtgtgca aatttacaat
     4681 ttcgacccca gcctggcgcc ggcaggcaag acggtagtga aagtttatta cgcttcggat
     4741 tatgagtact ggaaaaaact aatccgggat tctgcgcgtt atgaagcgga gaaagaactg
     4801 atcgccgacc aggtgatagc ggtcctagac caacgtttcc cggggttggc ttcccaggta
     4861 gagatgagtg atgtggccac accaatgacc ttcgagcact acactggtaa ctggcagggc
     4921 accttcatgg gatggcaaac ttcaaccaag actttgatca tgcgcatgag caagaccctg
     4981 ccgggactta agaacttcta tatggcgggg atgtgggtgg agccgggagg tggcgtaccc
     5041 acggcggcta tgtcgggccg caatgtaatc caaattatat gcaagctaga taagaggccg
     5101 ttcaaaacaa aacttccatg aagttaatat attgatggcg cggtgacctg ctcgggtaag
     5161 ttgagtctgg ttatcgaata tgtagaaaac aatatcgata caggaaaaat ggaatttcta
     5221 aaatctctta ggaaaaaatc atctgggaga aaaaactctc aactgctaaa acctactttt
     5281 ggacacaatg tcacaatgaa gcaacttgat aagcgccaaa ttactggatg gattggggtg
     5341 ggtttttcca ttgttatcac ctgcacctgg gcattttggg gcatcattga gaccttccat
     5401 gagggttggt attacgagtc ttttctctcc aacgtaggct taatgctcgc gcagtacctg
     5461 agcccaatgc tcatctttat gggagtcacg ctgatttcaa tctcttggcc tcggttcggc
     5521 gggggtctac acctgatctt agccttgctg gttgcctggt tcttccaggt atttaccaat
     5581 gcagccatct ttatgcttat cctgccactg attgggattg gagcgcttta ctggttcggt
     5641 cgtcccgagc cacgcaagat tgccctgtta ttggctgctg gacttcccat gctgatactt
     5701 gtcatatccg gtgtagagcc cgtcctccgt gtgtcccaac gctttgatga tggcaatcta
     5761 caagcccaat tggtatatgg aaatggagtc gatctcgtat gggcccctga tggacctgga
     5821 tggccgcgta caggggtaaa ctggcatgaa gcactacagg tctgccaaca tctcagtgaa
     5881 gatggtctta ccctttccct cgaatcacta gacatatggc gattgccgac cgtggacgaa
     5941 gccgtacgct ctatggctcg gcacggtcaa aacagcggtg gggtatggga tgcagaaatt
     6001 gccgaggcca cctatgaaac aactcccgac aaggaatctc cattgtggaa tatccattcc
     6061 caagtgatct actggtggac ggcgacaacg gttgatgaag aacaggcctt tatcattgtc
     6121 tatgatggaa aggtttggcc gagatcaaag caactcagtc tggcatattt agggtttcgg
     6181 tgtgtgaaat aacaatgctt acctgtgttc tgtaacagtt acgcataaat ttgtaattcc
     6241 ccagtgaaat accctcttgt ttaaaaagcg aacggggtac aacgacaata gaaattcctt
     6301 gatctatttg ccgtctcaaa tcttcgatat atggtaaaca aaagcatcca gaatataagc
     6361 ccaatgcatc attcgtcgaa gggatggaaa tctcatgaca aacatcactt gaaggatgat
     6421 ctatgatcta ataatataga tacatattaa tagataaggt taattattgg aaaagaggga
     6481 gagaaatgga tccaataaca ctcgttatta tagtgatagc cagttatctc ctgggcgcaa
     6541 tttctttcgc tcgtctgatt ccccggattg tcgatcccaa agctgatctt agcgatgtct
     6601 cgttgccagt cgaaaatagc gaagaaaaga tgcctatgct tgcaatgggc gccaacacag
     6661 catcaatcaa gtatgggccc aaggttggtt gcagtatagg actgctggat attttcaaag
     6721 tgttcttacc tacattgctg gttcgggtgc tttatccgga tgcatcttac tatctagcgg
     6781 ctgcaatagc agggtttatc ggccactgct ggcctgtata ttatcaattc aagggtgggc
     6841 ggggcatttc ggctttttac ggtgccttgt ttgctatcga cccgcttggc gcagtggttg
     6901 tcgccagcac tgccatgttt atcgggatgg ttctactgaa ggagttgctg gtggcttata
     6961 cgggtggggt gcttttaacc gttgtctggt ttgcaatcac ccgcggctgg gacttccacc
     7021 ttgcttacgc actagtaatc aatgtgcttt ttgtcgtcgc tatgatccca gagctgaaga
     7081 tgatccgaga aattcggcag aagtatggca gagaggatat gcgtaatagt atgagcacct
     7141 tcccaatggg ccaggcaatg ctcaagttga tggatgctct taaaattcaa cgcaagtaaa
     7201 cgagattggg gtggtatgga atatctagta tcgctgcata caaacaagtc acctgacaat
     7261 attgggaaca aagctctaaa tctacatcgc ctgtggcgga aaaaattcaa tataccgagg
     7321 acttacatat gtacatggaa tgccttcctg gacttccaga gcctgggtag cgttgctgtt
     7381 gatgccttgt cgaaggaatt agagcagatc atcaagccag agcggtccta cgctgtgcgc
     7441 tcatcggcca atttcgaaga caacaatcag cgttcatttg ccggacaatt caccagcctg
     7501 ctaaatgtac gcggtgtaga tcatgttgcc caggccgtac aatccatatg ggaggctacc
     7561 gagtctgaag gggttcaagt ctatctaaag caaagtatgc agccgaagga tcggctctta
     7621 atggcagtga ttatacaaga gatgattgaa ccggtaattt cgggtgtgtc attttcacgc
     7681 aacccgatca cgggcgcaga agaaattatt gtggaagcgg tgcgtgggga aggctcgcag
     7741 ttgatgcaaa ctggcattac gccaatgcgt tgggtgagcc gctgggacat catgctggat
     7801 aagccagatg gcgagtcaca gctaaacagg ctaatcgagc aaatagtgaa agaaaccaac
     7861 cagattgcga tcctccttcg gcgggatata gatctcgagt gggtgtacga tgggcgctca
     7921 ttgtattggg tacagcttag ggatatcact tcccttcaag aaggcgatgt ctacaacaat
     7981 cagattgcac gtgagatgac gccagggctc atcaaaccca tggtttggtc gatctcaatc
     8041 ccgatacata caggccagtg ggtgcgcatt ctaaatgaat tggtgagcaa tactcaaatt
     8101 gagccagcgc gccttgtgaa agcctttcat taccgtgcgt actacaacat ggggcagttt
     8161 ggccgcgttt ttgccagcct gggtatgcca cgcgactccc tggagcgcat gatgggcgta
     8221 ctgccgccgg gtgtgagccg accaaagttt atgccaggcc tctcattttt gccaaaagct
     8281 cctcaggtaa tccgcttcat tttggataag ctacgctttg ggcatcgcgc cgagcgggat
     8341 tatcccatcc tgcgccagga agcgcgcgca ttttccggac cactgacggc ttatgatcat
     8401 cccgaagacc tgctagaacg ggtagatcag atcgtttgtc tgcaggagaa gatcagctac
     8461 aatacgatca tgagcatctt gctcatgcag atttataatg gcttgctacg cagccattta
     8521 aaaaaccggg gggtggatcc cagtcagttt gacctcacgg accgatggga gagcatgcaa
     8581 gccttcgacc cgaacctgca cctagccaat ttacacgtcc aataccagac ccttgagccg
     8641 cagcttcagc acgaactaaa acaaggcaac ttgcaagccc tggaactgga agctagctta
     8701 tcggattttc gcagagattt ttaccaattc cttgaccgat ttggacacct gagcgatagc
     8761 acggtggatt ttactagcat accctggcgc gaatcgcctg atttgatcct gcgtctagtc
     8821 tgtgatttca gtccacgaga taagcaatcg gtcagtaaaa ttggcttgca ggatcttaag
     8881 accaagcgct tgagtttttt ttacctgcgc ttcatttacc agcgcgcccg tcagttcagc
     8941 ctcttccgcg aaatgtatag ctctctatat acctttactc tcatgttgct gcgcgatcac
     9001 tttcacgccc ttggaacaat tctgacggtt agtggcctgt tgccttcctg ggaggacata
     9061 ttcctgctat acatggaaga attgcgctct tgggtggaag gtgacgcaga tgggaaagac
     9121 tttgccgctc tggttacgca acgcaatgac gagatggagc gtagtcggca tgcgcaagtc
     9181 cccgatgtga ttcgcggaga cacgataccc ccgctcattc ctgccaatgc ccaaaagctg
     9241 caagggatcc caacatctgg gggctattat gctggacgtg tgaaagttat ccacgggctg
     9301 aatgagtttc acaaacttga gccaggcgac gtgctcgtaa ttccctactc agatgtgggg
     9361 tggacgcccc tgttcgctca cgctggagca gtcattgcag agtcgggtgg aatcctgtcc
     9421 cacagctcta ttatcgcccg cgaatataac atcccagccg tagtctcggt ccccactgct
     9481 atgaatctgc cggatggctc actggtagag gtggacgggt acaaagggct agtctacctg
     9541 aatgacggca gccagccagc tagcgcggtt caagagggag cgacagatgc cagttctgca
     9601 aactaatgac ttaaaaaaac aatatcaaat gggcaccaaa cgagtagacg ctctggcagg
     9661 agttgatttc ttggtggaaa agggcgagtt tgtcgctatt atgggaccct ccggtagtgg
     9721 caaatcgacc ttattgcact tggtcggcgg gctcgaccag ccatccggtg gagaagttta
     9781 cctggcaggt gcccggttat ctcaactgaa ggacaagaag gtaaccctgt tacgcagacg
     9841 caatattggt ttcgtcttcc agttcttcaa tctgctaccc actctcactg cagaggagaa
     9901 catcaccctg ccgctgttaa tcgatggtaa gaatcttaaa ggatttcagc aacggataaa
     9961 tacactgcta cagttaaccg ggctgagcga ccgtcgatgg cacaaaccag aacagctctc
    10021 cggcggcgag cagcagcggg tggccctggc gcgggccctg gtcactgagc ccgctatcgt
    10081 gctggcagat gagcccaccg gcaatcttga ctcgaagacc ggtacggcga ttatggaatt
    10141 gttacgaaaa tcctgcgatg agctggaaca gactatcgtt gtagtaaccc atgatcctcg
    10201 tgcggctatg tatgccgacc gagtcgtgtt tctgcgcgat gggcacatag atggcgagct
    10261 acgctttttg ccacttaccc cgctgaccga taagcttcat cagatcatcg agcgcatgac
    10321 taaactgggt gaataaatca tcttttgatg aatctcctgc gtttgctttc cctacgttcc
    10381 ttaaccaccc gccctatgcg ggtcttgcta agcacattcg gcatcgtcct gggtgtagct
    10441 gcaattattg gcattggcgt aaccaaccaa actgccttga actccgtcac gcagttgttt
    10501 gaaaacacat ctgggaaagc cgacctgatc attaccagca gggaggccga tgggcgcggc
    10561 atcccagaaa ccactgcaca gcgcatggag aagttttctg gtgtttttgc agccctacca
    10621 tctctgcaag tacagacagt gcttgccaat aacaagcaaa gtgacgatat cagtttgggc
    10681 ttcttcggta ctagtttggg aggattgctg ttatatggca ttgacccact tcgggatagc
    10741 caagcgcgcg actacagcat tgtcgcaggg cgattccttt cccctgatct attggcggat
    10801 gaggtggtgt tggtcaggtc atatgccgac caaaatgagc tcgaaatcgg caaatatatc
    10861 caatttgttg ccgccgggca agttgagaag ctgcgcatcg ttggtttgat agagagagaa
    10921 ggtccgggac aattgaataa tggcgccttc ggtgtgatgc ctctcggcac cgcccaaaga
    10981 ttgttcgatc gcagcgacga gatcgatcag gtcgacctgg ttatgtcgcc tgggttgtcc
    11041 agcgaccaac tggagagcga gcgtctgcgc ctgttgtctt acctgggtga ctcatactct
    11101 gtgatatatc cagcctcaca ggggaaacgc atgacccaga tgctcagcaa ctaccaaatt
    11161 ggtttaaatt tcttgtccgc tatggcgctt ttcgtgggcg ctttcttgat ctacaatgcc
    11221 ttttctatga ctgtggtcga gcgtacgcgc gaatttggca tgttacgaac gcttggcatg
    11281 acacgcctac aggtggcctt tcaggtactg gtggaggcct tttcaatggg cgccgtgggt
    11341 tctttactag gtatttgctt gggtctgctg atggcacgtg ggctttcgca actgatggct
    11401 gtcataatgg cacaagaaat gccccaggcc caggcaccgc aagaccttat ggtgattgcc
    11461 tttacgattg gggtgctcgt agcggtagct gcagctttac tccccgcctt gagagcagga
    11521 cgtgtctctc cactggaagc gctgcgcgtg cgggtccttg tgaatcaggg ctggctaatc
    11581 cgctatggat ggactgcagg gattgctctg ttggttgcgg ctgggtgggg attggtgttc
    11641 aatccctttc ccaacgaccc acagtttcgt tttggaagcc taatggtatt tgctcttttc
    11701 ctgggtggga cgctagttat ccctataagc ttctctgtat gggagcgcac cgcccgcccg
    11761 ttgatgatcg ttctgtttgg acgcgttggt caattgggca gccgaaatat tcaacgctca
    11821 cgcttgagaa ccacccttac ggtcgccgca ttgatgatcg gtgtcgcaat gatagtgatt
    11881 gtttgggtga tgaccgattc cttcaaaggc gacctggatg agtggctaaa gggctacatc
    11941 ggtggcgata tctatgtgac atctttggtt cccattcgag agaatatcgt ccggcgcatt
    12001 gaggtggtgc caggggtgga ggcagtcgca ccagtgcgtt attttgagat cgagtggcgg
    12061 cggcctggta atgactggga aacggtcaac tttatggcat tttccccagc ctcttataca
    12121 catgtcacca gctttgtttt tagccaatcc gactcagaat ctataaataa tatactgcgt
    12181 ggcgggcatg tcttcatctc cagtgtgatc gccgagaagc atggcctggc acccggtgac
    12241 cgtctgctga ttaagacccg cctgggggaa acacctttca ccgtcgcagc tgtcacagtt
    12301 gatttttcta accagggtat ggttatcaat ggatcatgga atgatatgga gcactacttc
    12361 cggctacgtg gggcaaacac gctgctgata aaggtaacac caggcacttc ccctgacgca
    12421 gtgggcgagg agattgattc ccgctatgga aaaggagaaa atctggtgat aatctcaaac
    12481 gagactctat tgggacgaat ttccaccctc atgcaacagg cattccgtat gtttgacgtg
    12541 cttgcattaa ttgcaatgct ggtaggcttt atggggatca caaatacgct caccatgaat
    12601 gtcatggagc gcacgcagga gattggtatg ttgcgcgcag tcggcatgac gcgtggtcag
    12661 gtagtgcgca tggtcttgtc tgagtcagca ttaatgggct tgataggcgg attgatgggc
    12721 ttggtgtttg gcgctgtcct ttcccgactt ttccttagcg cgatggccag catgtctggc
    12781 taccgtctga cctatgtact accgatagaa aacatcttgg aggctttatt gatcgctttc
    12841 ctgacctccc aactggcagc attcctgccc actttacgcg cctcacggac tcgcatcttg
    12901 gaagccattc aatacgagtg aggcagagca ttatatagtc aaagaggcta tacccaatac
    12961 ctccacaaca attatttatt caatattact caaggcagga gaatctaaat ccctcagtaa
    13021 aaaaagtgcc ctttgaggcg ctttttcctt tcggatctct gttattttgg taaggttttt
    13081 cgattagcta aacaactcta gcaattcacc tatggtttca aaggtgtaag taggcacaat
    13141 ctcgctgact aagtcccgct cgtcgaattc gccagtctta atcagcacag agcgaagacc
    13201 aaaataattt gccccctgta tatctgtagt gatgcgatcc ccaaccatga tgacgtcatt
    13261 tttgcctaaa tgcatgctgt caagtgccag ctcaaaaaca taaggattcg gcttgccgat
    13321 acttatcgcg cgcactcccg cagccctttc caacatcccc acccacgaac ctacgtttaa
    13381 ctcgatatcg cccatgctgg tatcgatcag ctcggattgc atggcaatca gcttcgcacc
    13441 ttttaccaga tgtctcaaga ctttattcag atgatcaaaa ttaaattggc tacgattgtc
    13501 tccaatcaca atgtactccg ggttatcggt gtcttggaca aatgctttga attcttgaat
    13561 accttctcga tctaccatca cccagcatga tcttggattt tgtgatctca agtagactgc
    13621 ggtagcatat gaagcggtga tcacctcatc ctcaagaaca tggaac
//
LOCUS       DBNGSX010000303        10504 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1064520, whole genome shotgun sequence.
ACCESSION   DBNGSX010000303 DBNGSX010000000
VERSION     DBNGSX010000303.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10504)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10504)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10504
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1064520"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..1458
                     /locus_tag="AC16CB_09180"
     CDS             304..1458
                     /locus_tag="AC16CB_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR3342680.1"
                     /translation="MSPEQNTKTTKLCPTCGTRLSEDAARCLVCGTELATTDKPSKPA
                     KAVQGSRLPEITLNLPAVLGLFILFIAIGAGLVYYAMRQTTPDTIPPTETPTITITPS
                     PTITTTPQPPTATNTPVPTPTPLSYKVASGDTCSSIAFAFGVSTQSIILLNSLPAACD
                     NLIIGQNLLIPHPTPTSTPQPTATMNAEESTRVACGEFEYTVQENDTLSSISANYAVA
                     IVVLKEYNGLVNDTVRSGQVIKIPLCQRAATAGPSPTPTLPPPYAAPSLLLPQDGAPF
                     TGTDDTVTLQWASVGTLRDNEAYAVTIVDVTGGQDRKLVDYVVDTKYNVPLSFRPTDN
                     LPHVIRWWVLTARQVGTDDSGNPIWEPAGASSAQRDFIWVGVALAVTPTP"
     gene            1739..2434
                     /locus_tag="AC16CB_09185"
     CDS             1739..2434
                     /locus_tag="AC16CB_09185"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR3342681.1"
                     /translation="MSVITISRQYGSGGDEIAELICHNTGYQLFDKHILARAAFEAGI
                     SDQEVIDYSEDNYMVKNFLARLFGISRPIAQLRVWKEGKDGVRVAEEIQLNEEHALSL
                     VRKAIEYAYEIGNIVIMGRGGQVILKDKPDVIHVRIEADIEDRLVRVRSYPEQKTQPF
                     SDSVEARRAAQDIIESFDTGSADYLKRFYGVDSSDPLLYHFIINTSKLSVEHSANLIT
                     QVAQQIKPIAEPA"
     gene            complement(2444..3751)
                     /locus_tag="AC16CB_09190"
     CDS             complement(2444..3751)
                     /locus_tag="AC16CB_09190"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HIR3342682.1"
                     /translation="MDLKEKLAASLPEWRERVRKLVKDSGDVVVGDVTISQVYGGMRD
                     VKALVTDISYVDPNEGIRLRGYTIPEVLEKLPKPEGATMPYVGGLYYLLLVGEIPTRD
                     QAVEVEEEWKRRATVPQFVFDALKAMPIDAHPMMLFSIAILALQNGSLFAKRYHEGMR
                     REEYWEPMLEDSLNLTAKLPAIAAYIYNLKYHERRIIAPDPKLDWAANFAQMVGIPTP
                     EYMDLSRLYFILHSDHESGNASAHATHLAASTLSDIYYAFSAGINSLAGPLHGLANQE
                     SLGWLLHIHDQFTGVPTREQLRQFAWDTLNAGQVIPGYGHAVLRKPDPRFTAQFEYAN
                     KYMPDDEIFQIAKMVYEIVPQVLIEQGKAKNPSPNVDAISGTLQYHYGLKEFDFYTVL
                     FGVGRSLGVSANAVWARALGQPIERPKSLTTALLEDIASKAKD"
     gene            complement(3791..4711)
                     /gene="mdh"
                     /locus_tag="AC16CB_09195"
     CDS             complement(3791..4711)
                     /gene="mdh"
                     /locus_tag="AC16CB_09195"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012660558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HIR3342683.1"
                     /translation="MKSKVSIIGAGMTGSTTAHWLAEREIADIVLVDVVEGMPQGKAL
                     DLQQAMPVIGKDVTVVGSNDYDATAGSDIIVITAGLPRKPGMSRDDLLAANATIVRQA
                     TEKTIEKSPNVIYVVLTNPLDAMAYLTMKTANISPERVVGQAGILDSARMRAFVAMEL
                     GVSVENVHCYVLGGHGDEMVPLTRHSNVAGIPLVDILPSDRLAAIVERTRKGGGEIVS
                     LLKTGSAYYAPAIAVTQMVDAILKDKHLIVDASAYLRGEYGLKDIFFGVPVQLGRKGV
                     EKVIEYRLNEEEMAAFQKSASGVAENLAKL"
     gene            complement(4787..5782)
                     /gene="trxB"
                     /locus_tag="AC16CB_09200"
     CDS             complement(4787..5782)
                     /gene="trxB"
                     /locus_tag="AC16CB_09200"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018461050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIR3342684.1"
                     /translation="MDFSLTNMASSQQTSPERVKVLIIGSGPAGLSAALYAARADLNP
                     VVLTGMELGGQVSLTYTVENYPGFPDGVGGTELVELFQKQAERFGARLEWDSATEVDL
                     SQRPFRVRTYNGEYLADALVITTGATPIHLDVPRESELTGRGVSYCATCDGWFFKEKD
                     VVIVGGGDSALEEGIFLTRYAKSVSIVHRRDALRAGAILQKRAMENPKIKFIWNSIVT
                     EILGDEAVNAVRLKDVVTGEERDYATDGVFIFIGHRPNTEIFKGQLELNERGYIVADQ
                     RMHTNVPGVFAAGEVADPIFRQVITSAGMGAAAAMQAIHFVEEQEESSAEPKVAA"
     gene            complement(5877..6815)
                     /locus_tag="AC16CB_09205"
     CDS             complement(5877..6815)
                     /locus_tag="AC16CB_09205"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002619004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 -
                     branched-chain-amino-acid:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HIR3342685.1"
                     /translation="MKSDFVWMDGDLVPFEEAKVHVINPTLHYGPGVFEGIRCYETPK
                     GPAVFRLRDHLERFIESIHILGVLKFPYTIDELQRGVHTTIRVNGFSECYVRPLMYLD
                     GPLGLNMDRSKARVFIAAWEWGPYLGEEAQEKGIHMMISSFTRLHPNISMTKAKITGN
                     YINSMLVKTLALRSGYDEAVILDPQGFVAECTGENIFMIRKGKLYTPPLSTVLDGITR
                     DTIMQLAEDMGIPVVEKMITRDQLYIADEVFISGTAAEIVGVRMIDHRRVGKGGVGPV
                     TQALLHAYHENLHGNGARSSEWLDLVPGEAGELTDF"
     gene            complement(6915..8612)
                     /locus_tag="AC16CB_09210"
     CDS             complement(6915..8612)
                     /locus_tag="AC16CB_09210"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC1 kinase family protein"
                     /protein_id="HIR3342686.1"
                     /translation="MIRQRYRLITGFFARLLLSFAYWDLILPRLGMRKWAKKTRPERL
                     RQSARAFRGLAIQLGGVMIKVGQFLSARVDVLPKEITDELEGLQDEVPPEQFEDIRRV
                     VEAEFGVPLSEKFTNFDEQPLAAASLGQVHSARLNLKSRESEEEQTPEQIERILDVVV
                     KVQRPNIENIITTDLTALRTVGEWLKRYRPIRLRADIPALLDEFTRVLYEEIDYLAEG
                     RNAETFAANFKSNVWVKVPTVMWTHTTRRTLTLENVLAIKITDYDAITSAGIDRSEVA
                     SRLLDVYLRQIFEDGFFHADPHPGNLFVSPRDEKDLESEVPWQLTFVDFGMVGRVPPQ
                     TREGLRELIIGVGTQDANRVVKAYQMLGVLLPGADLELLKKMEAKAFEQFWGKTMSEL
                     QQISTHQVRKFASEFRELLYSMPFQVPQDFVFLARCVGILSGMCTGLDPQFNVWTHLA
                     PYAQKLIAEEARVGWDFWWDEIKKQVKVAFSVPLKMDAILSQLERGDIAVRTPEVIRR
                     ITALDWSIRKLIGAILFAALLLSGTQFYLVGKSPLGEILLLGAGLCLLWVLFIQRRV"
     gene            9184..9510
                     /locus_tag="AC16CB_09215"
     CDS             9184..9510
                     /locus_tag="AC16CB_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342687.1"
                     /translation="MTYYWAYADRCWVISHLSEVRDDFLCEFISAFETLFEQMPVDRV
                     NFRHYSSVMRRPYGRLKKAIPLLHRNGKYYKITPRNGHKRRVSPESFPKFDPYRIADR
                     LPFADE"
     gene            9644..>10504
                     /locus_tag="AC16CB_09220"
     CDS             9644..>10504
                     /locus_tag="AC16CB_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR3342688.1"
                     /translation="MRWIVSHSIRYWYLLVSMLVGAIGNAALAAVVPVIIGEAINSVR
                     NTPFEQSVLLRMAFWIALSQVIRAVLQLARNFSAELIGQRLERDIRDELYVGLLGKSM
                     TFHSLQPVGDTMARATNDVREINLMFSPGINLVIGSASFLIMPLFLAPRYHPSLIITP
                     ALFMIGYFLSLWQYLRELDPVTGEVRKSFGHLNSRLAEALDGIETVKGMAQENSEVGL
                     FTRYARGYRDAFIRQIDIEARFLPLLLLGLAEAGGLLHAILLFWQGQLDVGAVVAYFG
                     LLQLFGFPTFV"
BASE COUNT         2713 a         2840 c         2285 g         2666 t
ORIGIN      
        1 cccgaggcag cgctatactc aatttcgcgg cacagcgcgc gtgtataatt tggaatcaag
       61 ccatccaaac ccgcgtaagt gttgaaatcg caataggcac agcggtgttt gcaaaatggg
      121 atgtgaaggt aaatgctgta agaatccatg ttagttgagt atatcatcct ctgctttccc
      181 cgggcgacat gggaaaggct accattaaag aaatactatg ccttatcgca catcaaacac
      241 aatactggta taatcgccag agtctgcgcc agaacctaat agaaaatctg gctggaaatg
      301 aatatgagtc ctgaacaaaa cacaaaaacc accaaactct gccccacctg cggaacacgc
      361 ctgagcgagg atgccgctcg ctgcttggtg tgtggcacag aactagcaac cacagataaa
      421 ccctctaagc cagctaaggc agttcaaggc agccgtctgc cagagatcac tctcaacttg
      481 ccagctgtgc tgggcctatt catcttattc atcgcaattg gtgctggatt ggtctattat
      541 gcaatgcgcc aaacaactcc agatacaatt ccgcccacag aaacacctac cataacaatc
      601 acaccttctc cgaccataac tactactcca caaccgccaa ctgcgacgaa tacgcccgta
      661 ccaacgccaa cgcctcttag ttacaaggta gcttctggcg atacctgttc atcaatcgca
      721 ttcgcattcg gcgtctccac gcagagcatt atcctgctca acagcttgcc agctgcctgt
      781 gacaatctaa ttattgggca aaatctgttg attcctcacc ctacacctac cagcacacca
      841 cagcccacag ccaccatgaa cgccgaagaa tcgacacgcg ttgcttgcgg agaatttgaa
      901 tataccgtgc aagaaaacga tacattgagc agtatctccg ctaattacgc tgtcgccatc
      961 gtagtgctca aggaatacaa cggtctggta aatgacacag tccgctctgg ccaggtaatt
     1021 aaaattccgc tctgccagcg agccgccact gcgggcccgt cacccacacc cactcttccg
     1081 ccaccctatg ctgcgcccag tttactgcta cctcaggatg gcgcgccctt tacaggcacc
     1141 gatgatactg tgacgcttca atgggcttcg gtaggaacac tgcgagacaa cgaagcttac
     1201 gcagttacta ttgtagatgt caccggcggt caggaccgca aacttgtgga ttacgttgta
     1261 gataccaaat acaatgtacc gctttccttc cggcctactg acaacctccc ccatgttata
     1321 cgctggtggg ttctgactgc tcgccaggta ggcaccgatg acagcggtaa ccccatttgg
     1381 gagccagccg gcgcatccag cgctcagcga gacttcattt gggtcggcgt cgcgttggca
     1441 gtcacaccaa caccataatt atttacgagt aatacggttc gtgagttagt gtgggtagtc
     1501 ggcgagattg ttcgactacc cgcatatttt caattgcgat aagcccgatt ttgctgaatg
     1561 cttcttgatt aagcacagca actatgctat attgaattta aacaggaacg tggttccagc
     1621 agcaagccag ttttaggcgg gaaccttaat tccaatgcag aatttcatca aagcccgccg
     1681 tattttatgg cgggctttat cacatatacg aatttaatta aggatgagag gttttgccat
     1741 gtctgttata actatctccc gacaatatgg cagtggcgga gatgagatcg ctgaattaat
     1801 ctgccacaac acaggctacc aactattcga caaacacatt ctcgcaagag cggcgttcga
     1861 agctggcatt tccgatcagg aagttatcga ctactccgaa gataactata tggttaagaa
     1921 cttccttgca cgcctgtttg gtatctctcg cccgatcgcc caattacgcg tctggaagga
     1981 gggcaaggat ggggtgcggg ttgctgagga gatacaactc aatgaagagc atgcgctctc
     2041 gcttgttagg aaagctatcg aatacgccta cgagatcggc aatatcgtta ttatgggtcg
     2101 tggcggacag gttatcttga aagataaacc ggatgtgatc catgtccgca ttgaagccga
     2161 tattgaagat cgcctcgtgc gggtcagaag ctatccagag cagaaaacac aacccttcag
     2221 cgactcggtc gaagcccgaa gggcagccca ggatatcata gaatcatttg acacaggctc
     2281 ggctgattac ctgaaacgct tttacggtgt cgattcgtcc gatccgttgc tatatcactt
     2341 catcatcaat accagcaagt tgagcgtaga acactctgcc aatctcatca cccaggtcgc
     2401 gcaacagatt aagcccatcg cagagcctgc ctgagacggc gaattagtcc ttcgccttgc
     2461 tggcaatatc ttctaataaa gcagtagtca aagacttagg ccgctcaatt ggctggccca
     2521 gcgcgcgcgc ccatactgca ttagcactta cgcccaacga acgtcccaca ccaaacaaca
     2581 cggtataaaa atcgaactct ttcaagccgt aatgatattg aagtgttcca ctgatggcat
     2641 ctacattggg tgaagggttt ttagctttcc cttgctcaat gagcacctga ggcacaatct
     2701 catataccat tttcgcaatc tggaatatct catcatcagg catgtactta ttggcgtatt
     2761 cgaactgcgc cgtgaagcgt ggatcgggct tgcgtaacac cgcatgacca taacccggga
     2821 ttacctgccc agcattgaga gtatcccaag caaattggcg taattgttct cgtgtgggga
     2881 caccagtaaa ttgatcatga atatgcaaga gccagcctaa agattcctgg ttagccaatc
     2941 cgtgcagtgg tccagccaaa ctattgatac ctgccgagaa agcataatag atatccgata
     3001 gtgttgatgc cgccaaatgt gttgcgtggg cgctggcgtt accgctctcg tgatccgagt
     3061 gaaggataaa gtacaaccgg ctcagatcca tgtattcagg ggttgggatg ccaaccattt
     3121 gagcaaaatt cgccgcccag tccagctttg gatcaggtgc gattattcgc cgttcgtggt
     3181 atttcaaatt atagatatac gccgcaatag cagggagctt agctgtcagg tttaggctat
     3241 cctccagcat gggttcccag tactcttcac gccgcattcc ttcatgataa cgcttggcaa
     3301 aaagcgaccc attttgcagc gcgagaatag cgatagagaa aagcatcatg gggtgcgcgt
     3361 caatcggcat agctttcaga gcatcgaata caaattgagg gacggtcgca cgtcttttcc
     3421 attcttcttc tacctcaacc gcctgatccc tggttgggat ctctccaacc aatagaaggt
     3481 agtaaagacc gccaacataa ggcatcgttg cgccttcagg cttcggtaac ttttcaagca
     3541 cttcgggtat cgtataaccc ctcaagcgga tgccttcatt cggatcaacg taagagatat
     3601 cggtgaccag ggcttttaca tcgcgcatac caccatagac ttgactaata gtcacatcac
     3661 caacgacgac atccccacta tctttgacta attttcttac ccgctcgcgc cattctggca
     3721 acgaagctgc taacttttct ttaagatcca tttttttagt atcctttgac gaaattctat
     3781 ttacttaaaa ctacagtttg gcgagatttt ctgctacacc actcgccgac ttctggaagg
     3841 cagccatctc ttcctcgttc aagcgatatt caatcacttt ctcaacacct ttgcgtccta
     3901 actgaactgg cacgccgaaa aagatatcct ttagcccata ttcgccccgc aagtaagcag
     3961 aagcatccac gatcaaatgc ttatccttca ggatcgcgtc caccatctgg gttacggcga
     4021 tggctggggc ataatatgct gatcccgttt ttaataggct gacaatctcc ccgccaccct
     4081 tacgtgtacg ctccacaatt gccgccaaac gatctgaagg aaggatatcc actaagggga
     4141 tgccagccac attcgaatga cgtgtcagcg gtaccatctc atcaccatgt ccgcccagca
     4201 cgtagcagtg tacattttcc acacttaccc ccagctccat agctacaaag gcgcgcatgc
     4261 gcgccgaatc aagaattcct gcctggccaa caacccgttc aggtgaaata ttagcagttt
     4321 tcatggttaa gtaggccatt gcatcgagtg gattggtcag caccacatag atcacattgg
     4381 gcgatttctc aatggttttt tctgtcgctt ggcgcacaat tgtagcattt gctgccagga
     4441 ggtcgtcccg gctcattccc ggtttgcgcg gcaagccagc agtgattact ataatgtctg
     4501 aacccgctgt agcatcataa tcattgctac ccactacggt cacatccttg ccaatcaccg
     4561 gcattgcctg ttgcaagtcc agcgcctttc cctgaggcat gccctccact acatccacca
     4621 ggacaatgtc agcaatttct cgctctgcca gccaatgggc agtggtagac ccagtcatac
     4681 ctgccccaat aatagaaact ttcgatttca tgcatttctc tcctgaataa aaataacgat
     4741 ggttcagaag atatactcct gaaccactgc ttaaatcata acaaactcat gccgccactt
     4801 ttggttcggc tgaggattct tcttgttctt caacaaagtg tatcgcttgc atagccgccg
     4861 ccgcacccat accggccgac gtgatcacct gccggaatat ggggtcagct acctcaccgg
     4921 ctgcaaacac tcctgggaca ttggtatgca tacgctgatc agcaacaata tatccacgtt
     4981 cgttcagttc gagttgccct ttgaagattt cagtatttgg cctgtgacct ataaatataa
     5041 atacgccgtc ggtagcgtaa tctctctctt cgcccgtgac aacatctttc aacctcaccg
     5101 cattgaccgc ttcatctccc aatatctcgg tgacaataga attccagata aatttgatct
     5161 ttggattttc catcgcccgt ttttgtaaaa tcgccccagc tcgtaaagcg tcccggcggt
     5221 ggacgatgga gactgatttt gcatagcggg tcaagaagat tccctcttca agggcgctat
     5281 cacctcctcc aacgatcacg acatcctttt ccttgaagaa ccagccatcg caggttgcac
     5341 agtaagaaac accccgcccg gttagttcgc tctccctagg gacatccaga tggatcggag
     5401 ttgctcccgt tgtgatgacc aatgcatccg ccaagtactc accattgtag gtgcgaacac
     5461 ggaaaggacg ctgagagaga tctacttcgg tagccgaatc ccactccaat cgagctccaa
     5521 aacgctcggc ttgcttctga aatagctcaa ctaactcggt tccccccact ccatccggaa
     5581 aaccagggta attttcaact gtataggtga gcgatacttg cccacccagt tccataccag
     5641 tcaatacgac cgggtttaga tccgctcgag ctgcatacaa agccgctgag agtccagccg
     5701 gtccagaacc gataattaga acctttactc tctcagggct ggtttgctgg cttgaagcca
     5761 tatttgtcaa actaaaatcc atgtatcata tcctcatcgt aattctatca aataatagat
     5821 ggagaattac agttgctctc attttatcag atgcgccgac aagccaaaaa gttgcattag
     5881 aaatctgtta attctcccgc ttctccgggg acaaggtcca gccactcact cgatcgagcg
     5941 ccgttcccat gaagattctc atgataggca tggagcaaag cctgggttac aggcccaact
     6001 ccacccttac caactcttcg atggtcgatc atgcgcaccc caacaatctc ggctgcggta
     6061 cccgatataa atacttcatc tgcgatatat aactgatcgc gtgtaatcat cttttctact
     6121 accggaattc ccatatcttc ggcaagttgc atgatggtat cgcgagtgat accatccaga
     6181 actgtggaca atggcggggt gtataacttc cctttgcgga tcataaagat attctcgccc
     6241 gtacactcgg ccacaaatcc ttgcgggtcg aggatgacag cttcatcata ccccgagcgc
     6301 aacgcaagcg tctttaccag catagaattg atatagttgc ccgttatttt ggctttggtc
     6361 attgagatat tgggatgcaa gcgggtgaac gaagaaatca tcatgtggat gcccttctcc
     6421 tgcgcctctt cccccaaata tggtccccac tcccatgctg caatgaaaac ccgcgctttg
     6481 gagcgatcca tattaagacc caaaggtcca tccagataca tcaacgggcg cacgtaacac
     6541 tcgctaaagc catttactcg gatggtagtg tgaacaccgc gttgcaattc atcgattgta
     6601 tatgggaatt tgagcactcc aagtatatga attgattcaa taaaacgctc caggtggtct
     6661 cgtaaacgaa acacagcagg ccctttcggc gtctcataac agcggattcc ttcaaataca
     6721 cctgggccgt agtgcaaagt tggattgatc acgtgaacct tcgcctcctc aaaaggcaca
     6781 aggtctccat ccatccaaac aaaatcggat ttcatttaga actcctttat tttcgcatgt
     6841 agaaccccaa tagacggatc agattatttg ttcggtggtt atttcaaatc caggtatcgc
     6901 aataataata tactttaaac tcttctttgt atgaagagca cccataggag acacaaacct
     6961 gcgcctagca ataaaatttc ccctaaaggg ctctttccaa ccagataaaa ctgggttcca
     7021 ctcaaaagca acgcagcaaa caggatagca ccaataagct tgcggataga ccaatccagc
     7081 gctgttatcc tgcggatgac ctccggagtg cgcacagcga tatccccccg ctctaattgg
     7141 gataatatcg catccatctt caatggaact gaaaacgcca ctttcacctg ctttttgatc
     7201 tcgtcccacc aaaaatccca gccaactctg gcctcctcag ctattaactt ttgtgcatac
     7261 ggtgccagat gtgtccacac gttgaattgc gggtcaagac cagtacacat tcccgaaagt
     7321 atccccacgc aacgagctag gaatacaaaa tcttgaggca cttgaaaagg catggagtag
     7381 agcaactcgc gaaattcact ggcaaatttg cgcacttggt gggtgctgat ttgctgaagc
     7441 tcactcatcg tttttcccca gaattgttcg aaagccttgg cctccatttt cttcaacaat
     7501 tccaagtccg ctcctgggag aaggacacct aacatctggt atgccttaac cacccgattc
     7561 gcatcctgtg tgcccacgcc gataatcaat tcacgcaacc cttctcgggt ttgaggtggg
     7621 acgcgcccaa ccataccaaa atcaacaaac gtgagctgcc agggtacttc cgactcaagg
     7681 tctttttcat cccttggcga gacgaacaaa ttacctgggt gtggatcggc gtgaaagaaa
     7741 ccatcctcga aaatctggcg caggtagaca tccagcaatc gagaagccac ttcggatcga
     7801 tcaatgcctg cggaggtaat ggcatcataa tctgtaatct tgatagccaa cacattttcc
     7861 agcgtcaatg tgcggcgcgt ggtgtgcgtc cacatcacgg ttgggacttt aacccataca
     7921 ttggacttga agttcgccgc aaatgtctca gcattacgac cctctgccag gtaatcgatc
     7981 tcctcgtaga ggacccgcgt aaactcatct aacagggcag gaatatccgc ccgtaggcgg
     8041 ataggtcgat agcgtttgag ccactccccg acggtacgca aggcagtcaa atcggtggtg
     8101 atgatattct caatatttgg acgctgaact ttcaccacca catctaaaat gcgttctatt
     8161 tgctctgggg tttgctcttc ttcggattct ctggatttca gattgagcct tgcgctgtgc
     8221 acctgtccta aagatgcggc agctaatggc tgctcgtcaa agtttgtaaa tttctcagag
     8281 agagggacgc caaattccgc ttcaaccacg cggcgaatat cctcaaattg ctctggaggt
     8341 acctcgtctt gcaagccttc tagctcgtcg gtgatctctt tcggcagaac atcaaccctg
     8401 gcagagagaa attgtcccac tttgatcatc accccgccca attggatcgc taaaccccga
     8461 aacgccctgg cgctttgcct taagcgctca gggcgtgttt tctttgccca ttttctcatc
     8521 cccaagcggg ggagaatgag atcccagtaa gcaaaactaa gcagcagacg cgcaaaaaac
     8581 cccgtaatga gtcggtaccg ctggcggatc atagcatcag ggattatacc cttttagatg
     8641 atcaacttta aaactatgag gtattacgtg gaacaagcga aacaaacgat atatgaccct
     8701 atgaagccca aacaacccaa ggatcattga aaccaaaaag gatatatatg aaggttataa
     8761 tctactcaat ccaatggaat ttatcccctg tttgtgtggt atcctctcaa catgccccct
     8821 cctaaccctg aagattttgc ctctttttac cagaatttca actctccgat aaccagacat
     8881 gattgcggca ataaatgcgc cccgtataac gagcatggcg ttcccttttg ctgtgataca
     8941 cgccatacga tccccactgc gtaccaaaac gaatgggaat atcttcaggt aaatactggt
     9001 ctctggcatc tttttcagcc aaaccaaccc caaagacctg ggcgcatcct ctctgatgtc
     9061 cctgatggac aagtcatgat cgagtgccag gggcacaaat tatgccagcg aaatttccgc
     9121 tcgattactt gcaggagctt cccgtttttt ccttacatta ctcgccagga tgaatttatc
     9181 ggtatgacat actattgggc ttatgccgat cgatgctggg tgatcagcca cttatccgaa
     9241 gtccgggacg attttctctg cgagtttata tccgccttcg agaccctttt tgagcaaatg
     9301 ccagttgaca gggtaaattt ccgccactac tcttcagtta tgcgccgccc ctacggccgt
     9361 ttgaaaaagg caatccctct tctccatcgt aacgggaaat attataagat tacgccgcgt
     9421 aatggccata agcggcgtgt ctctccagag agtttcccaa aattcgaccc ttatcgcatt
     9481 gctgatagat tgccatttgc tgacgaatga cccgcctaga taaacttacc ccgtgccgcc
     9541 ggcactcagc tgatgaatta aattgcaatc ctcaatagct aactcacctt ccgtaaccga
     9601 atttagccta ccccgacgcc atcccaccaa ccgtcgaggt ccgatgcgat ggattgtttc
     9661 acatagcata cgctattggt atctgcttgt ctctatgctg gtcggcgcaa ttggaaatgc
     9721 agcgctggct gccgttgtac cagtcataat tggtgaggcg atcaactctg tacgcaatac
     9781 accctttgag caatctgtgt tattgcgtat ggcgttttgg atcgctcttt cacaagtcat
     9841 acgggcggtg ttgcaactgg ctcgtaattt cagcgctgag ttgattggtc aacgcttaga
     9901 gcgcgatatc cgcgatgagc tttatgtcgg cctattggga aaaagcatga ctttccatag
     9961 cctgcagcct gttggcgaca ccatggcaag ggcaaccaac gatgtccgcg aaatcaatct
    10021 tatgttcagc ccaggcatca acctggtgat tggctcggct agttttctta ttatgccgct
    10081 attcctcgcc ccacgttatc acccttccct gatcattaca ccagccttgt ttatgatcgg
    10141 atactttctc tccttgtggc agtacctccg cgaattagat cccgtcactg gtgaagttcg
    10201 gaaatcattt gggcatttaa atagccgatt ggctgaagca ttggatggga ttgagaccgt
    10261 taaagggatg gcccaggaga acagcgaggt aggtcttttc acgcgctatg cccgcgggta
    10321 tcgggatgca ttcatccgcc agattgatat cgaagcccgc tttttaccac ttttactgtt
    10381 aggtctagcc gaagctggtg gcttattaca tgccatcctg ctattttggc aagggcaact
    10441 agatgtgggt gcagtggtcg cctattttgg acttctccaa ctctttggct tcccaacctt
    10501 tgtc
//
LOCUS       DBNGSX010000304         2154 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_371447, whole genome shotgun sequence.
ACCESSION   DBNGSX010000304 DBNGSX010000000
VERSION     DBNGSX010000304.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_371447"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..878
                     /locus_tag="AC16CB_09225"
     CDS             75..878
                     /locus_tag="AC16CB_09225"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HIR3342689.1"
                     /translation="MDRYRVIPESRVELSDYDPGDTAEFSGDKDEGKDRLDELNDQLE
                     ALQELLYAEHKHKLLIVLQGMDTSGKDGVIRHVFDGVNPQGVRVANFKVPTPQELDHD
                     YLWRVHQQVPGRGEIVIFNRSHYEDVLVVRVHELVPKERWSRRYQQINDFERLLVEEG
                     TTILKFFLHIDQQEQKERLQARLDDPNKRWKFNVGDLKERKLWDEYRQAYEDVLSQTS
                     LEVAPWYVVPSNRKWYRNLVIASVIVEALEKLNMSYPEPEDDLSSIKIE"
     gene            complement(865..1683)
                     /locus_tag="AC16CB_09230"
     CDS             complement(865..1683)
                     /locus_tag="AC16CB_09230"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3342690.1"
                     /translation="MQNHNRFYEKIAIVTGASDGIGRATALALAKRGAHLAIAARRKD
                     LLDSLASEIKMYDRDVLVAEVDVTDIHHVNHLVDEVINRWGQVDILISNAGEYIRGYI
                     DTLTIQDIQRSMAVNFYGGVYAIMAVLPHMQKRKSGHIVVVTSMDGKKGLPKDAPYAA
                     AKFALTGFVEVFRQELCCDSGIYISNVLPGRVNTKMIESLRVPWISAKISPEAVTRAI
                     LFAIEKRKPEVIIPPQAILLHYINVLSPRMGDYLARVLHLEGWEDQREGDSTLS"
     gene            complement(1670..>2154)
                     /locus_tag="AC16CB_09235"
     CDS             complement(1670..>2154)
                     /locus_tag="AC16CB_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342691.1"
                     /translation="EMIGMDSLLPKIVLNFDGRVGAEVPVIRSKLHGHRGISSFNPQL
                     VEFVPLDAPYYHFPVSCATDAQAREVIAAFSRSVALKNPSDPRNVVFTILPTHGVVIV
                     EKWVPGSVAFQTIWEYFDHGHLVLDSHVPQGPVDFISKSGRQVLKDTASMVSHLHAKP
                     "
BASE COUNT          522 a          546 c          591 g          495 t
ORIGIN      
        1 gctagacagg taaggttttt aggaatagac ttacagcttg aaagatttac atcaagaagg
       61 aggagacttg ctctatggac cgttaccgag tcattcccga gtcacgggtt gagttgagcg
      121 actacgaccc aggcgatacc gccgagtttt cgggcgataa agatgagggt aaagacaggc
      181 tggatgagct taacgaccag ctggaggcgc tgcaggaatt gctctacgcc gagcacaagc
      241 ataagctcct gatcgtattg caggggatgg atactagcgg gaaggatggc gttatccgcc
      301 atgtattcga cggcgtgaac ccccagggcg tgcgcgtggc aaatttcaag gttcctaccc
      361 cgcaggagct tgaccatgat tatctatggc gcgtccatca gcaagtgcca gggagaggag
      421 agatcgtcat cttcaaccgc tcgcattacg aggacgtgct ggttgtcagg gtgcacgaac
      481 tggtgccaaa ggaacgctgg tcgcgccgct accagcaaat taacgatttt gagcgcctgc
      541 tggttgaaga ggggacgacc atcctgaagt tctttctgca cattgatcag caggaacaga
      601 aagagcgcct gcaagcgcgc ctggatgacc cgaataagcg ttggaagttc aatgtgggcg
      661 atctcaagga acgcaaattg tgggatgaat ataggcaggc ttacgaggat gttctcagcc
      721 agaccagtct tgaagttgct ccttggtatg ttgtcccctc caaccgtaag tggtatcgca
      781 acctggtcat cgccagcgtg attgtagaag cgctcgaaaa attgaacatg agctatccag
      841 agccagagga tgatctaagc agcattaaga tagagtagaa tctccttctc tctggtcttc
      901 ccaaccctcc aggtggagga cgcgtgccag gtaatctccc atgcgagggg agagcacatt
      961 gatgtaatgc agcaaaatgg cttgaggcgg gatgatgact tcaggcttgc gtttctcgat
     1021 agcgaacagg attgccctgg ttacggcctc aggcgagatt ttggctgata tccatggcac
     1081 tctaaggctc tcgatcatct tcgtattcac cctgcccggc aagacattgc tgatgtagat
     1141 accagaatcg caacaaagct cctggcggaa tacctctaca aagccggtca gagcgaactt
     1201 tgcagcggcg tagggggcgt cctttggcag tcctttcttc ccatccatac tggtcacgac
     1261 aacgatatga ccgctctttc tcttttgcat gtgcggtagc acggccatga tggcatatac
     1321 cccgccataa aaattcaccg ccattgatcg ctggatgtcc tgtatggtca gtgtatcaat
     1381 gtagccgcgg atgtattcgc cggcattaga gatgaggatg tccacttgtc cccagcgatt
     1441 tatcacttca tccaccagat ggttcacatg gtggatatct gtcacatcta cctctgcaac
     1501 caggacgtct ctatcataca ttttgatctc cgaggctagc gagtccagga gatccttgcg
     1561 gcgagcggca atggccaggt gtgcgcctcg ttttgccagg gccagcgcag tcgcgcggcc
     1621 aatgccatcc gaggccccag tgacgatggc gattttttca taaaaccgat tatggttttg
     1681 catggagatg gctgaccatt gatgcagtat ctttcaaaac ctgcctccct gatttcgaaa
     1741 tgaaatccac agggccttga gggacgtggc tgtccaagac cagatggccg tggtcgaaat
     1801 attcccagat ggtttgaaat gcaacgctgc ccggcaccca cttctccacg atcaccacac
     1861 cgtgggtggg caggatggtg aacacaacat tccgtggatc cgaggggttc ttgagcgcta
     1921 ccgagcgcga gaaggccgct atgacctccc gtgcttgggc atctgtggcg caagacaccg
     1981 gaaaatggta gtatggtgca tccaggggga caaattcaac taactgcggg ttaaacgatg
     2041 agatgccccg atgtccatga agtttcgagc gaatcaccgg cacctcagcc cccactctgc
     2101 cgtcgaaatt cagcacaatt ttgggtaaga ggctgtccat gccaatcatt tcaa
//
LOCUS       DBNGSX010000305         8475 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_510585, whole genome shotgun sequence.
ACCESSION   DBNGSX010000305 DBNGSX010000000
VERSION     DBNGSX010000305.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8475)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8475)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8475
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_510585"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="AC16CB_09240"
     CDS             <1..278
                     /locus_tag="AC16CB_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342692.1"
                     /translation="GDDPGQTSPNSSDPGQLNPSCDKDCIPTIDQVGPEGEANPMPIH
                     ENETHMHGVKRENSEEANHAGENAGRNSFGQEASDSTGRFGSGSSSP"
     gene            544..1134
                     /locus_tag="AC16CB_09245"
     CDS             544..1134
                     /locus_tag="AC16CB_09245"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3342693.1"
                     /translation="MSDWNEVDDIQLLKFAQEGNTEAFGAIYERFADKVYRYLNAHLG
                     NSLDAEDITEEVFIRVWRSLPDYHERGTPFLSYVFRIARNALIDHYRRERRTAGQLSS
                     EDVMIADHKPGPGEVVSQQMEHKELREALDDLKDDYRNVLIMRFLSDMSPEETAHAMG
                     RSAGAVRVLQHRALAALRTKFEKSKGKRDGRKARRD"
     gene            1109..2260
                     /locus_tag="AC16CB_09250"
     CDS             1109..2260
                     /locus_tag="AC16CB_09250"
                     /inference="COORDINATES: protein motif:HMM:NF039823.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HIR3342694.1"
                     /translation="MAEKREGIEQILQTCLELIRSGQETVDSALARYPDHADELSSLL
                     ETATWLQERRDALAPRPEFIAASRQRLLARIEQEKAVSTPKSLRTVGASVWNTLLSLF
                     AQKRFAYQFVVATILLVFLISTTSGVAAAAQHSIPGDTLYGVKMSLEQIQLALSFSEA
                     RRAQYYVTFAERRLVEVQSLVIEGRYEYLHNTVDQFYFQANEASRLLRSLGTTNTLLA
                     RELANKLRNVVEGQISLMPVLSQAAPPDSRSEIERLMQMAELVKSEAQNIEALMGGTP
                     LPTITPTLITITPVPPTPVPTKTPVPTTAPVVTVAPLSTPLSAKTEAAPTLTVRPSRT
                     PVPTLQNSGKKPTPTPTKAGPANPTRKPTKEKKEPPGLENRPPKPTKKS"
     gene            2468..5110
                     /locus_tag="AC16CB_09255"
     CDS             2468..5110
                     /locus_tag="AC16CB_09255"
                     /inference="COORDINATES: protein motif:HMM:NF015140.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HIR3342695.1"
                     /translation="MLGILRKSRQVDRGQGMVEFALVLPLLLLVIFGVIETGRLLFVY
                     SVVFTSTREAARYGSASGLLTGALNQYQDCNGIRAAAKRVGVFAGIQDSDITITYDRV
                     TGTGVSPLGSCPIGGSGPKLTLGNRVTVRVTVNYRPIVPIPNFPSFPITSTSSRTILK
                     NISIQGTPVVSSSTLPVVYLSPATFDQIEGDDGNTSIVTVVALLSGGPAEQNVTLNFS
                     LSGSATKGVDYTVDKSSPVIIPSGQSSTEIKITVIGDDLSEFNETVVLTIESTINAER
                     GDPSIFELAILDDDSDPLVIFAPPYPLLPIQENLGFIDFEVLLVNPDGSPATSGKDVE
                     VSFSLAGDATEVIDYKPPPSTFIIPAGSSSKTLRFDIVEDTIDEPDEIFIVTMVSFVN
                     ANPGANKVQTATIKDNDDPPYVSFTTDSQIVSESIGIVLITAWLQDASGAEVKSGLPV
                     SVVFNVEGTASNDDDYTISSSPLEFSAGSSRADIVVYVTNDSVVETDETVIVTMGALT
                     NAQQGSPSKQTVTITPNPTVFFTLASQSVHENVLKADIEVQLLPAQPGDVSVAFSLAG
                     TATQGKDYTLETNSPLVFSAGQTTGTISLILEMDKLDEADETVQVTLSSPSGGAVLGT
                     PKVHLLTLIDDNPQPFVYFSSSDQSVDENAGDVNVLVQLSAASSLPVTVPLSLGGSAA
                     KGSDYIFNQTSVVIPAGITSTLLTLQVIDDILPETFETIVLAMETPTNAVVGTPGTHA
                     ITINSNDLPTCNIFKSTEITLNAPGMYLSLPLSTSVSDTLILSRLTISWPSGAPNSPM
                     FDKVLFNNFEVYDSNHPHSPVTVTNWNGLPSNRQLNPNPSTIKLTFTRALDPGTYTLT
                     LLFHNETTGFDCTPIQSSATIPQP"
     gene            5124..6545
                     /locus_tag="AC16CB_09260"
     CDS             5124..6545
                     /locus_tag="AC16CB_09260"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HIR3342696.1"
                     /translation="MRTYYWIPSLITLLTFLALLLPAGLAHADVSSGTVITVNSAVDD
                     ELNGTCSLREAIQAANTDSAVDACPAGDGADTILLGAEIYQLSIKGTGEEYAQTGDLD
                     IREDLVIRGQGMNSTVVDGGGIDRVFHIYWTDPHPVVHFEHLTIQNGNVTKDLYGGGG
                     ILNEYGTITFQAVSLENNHSIYDGVGSAVGGGMDNLGTATLINSYVSNNGADDGGGIF
                     NSGTLSIYRSTLNNNTATLDEGGALDNYGGTVTLEDVTISGNIALSGSGGGIFSDGTL
                     FISNTTISGNTASINGGALYNASDATLQNVTISGNSAASGGGIYNTGHLDITNSTIVE
                     NSAISAEPPNGANIDNEDTVTIENTIIALGQIAPNCAGLAGLTSLGHNLEDANTCNLD
                     HADDHPGVGDPSIDVLANRGGPTWTHALNSSSPAIDTGNDDTCPSTDQRGANRAGEYC
                     DIGAYEYAGEFSIENVFLPLITR"
     gene            6599..7042
                     /locus_tag="AC16CB_09265"
     CDS             6599..7042
                     /locus_tag="AC16CB_09265"
                     /inference="COORDINATES: protein motif:HMM:NF019428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HIR3342697.1"
                     /translation="MKDKALRRYSEKGQSLTEFAISLLILLLLLAGAVDAGRAFFTFM
                     SLRDAVQEGALWGSYNPTETELIKDRVRKASNLVQAIADDPNATLDIDVDIDDAACNG
                     NGITVQATYENFPITMPFLGTIIGSQKVPISASVTDTILVPPCEK"
     gene            7065..8459
                     /locus_tag="AC16CB_09270"
     CDS             7065..8459
                     /locus_tag="AC16CB_09270"
                     /inference="COORDINATES: protein motif:HMM:NF024792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HIR3342698.1"
                     /translation="MLDTYTQEKGQALVLITLAMVVLLGFTALAIDGGMVYSDRRHAQ
                     NAADASSLAGGSAAALQLENDHITYGTWGCNKGDILDAMDAGEGAAIDRADDNGFSID
                     ANITDYHGVMTDCGSEPYGPWEDKFIDIHTHISTTTPTSFAHFVYGGKLMSRVHSVTR
                     IRPRMAFGFGASILALNDDPNCDGNQNGVVFDGNNEVQVEGGGITSNGCLKGSGSTLD
                     VEVVGGDIIHVGEIETQHVDTFSPNPESGNGVKLPDFAVAYPKPDCSEVDDFDHPSTE
                     FRNNAGGTYLDPEFIPAGNYSAIKMNGTVVLEGGGLYCLYGDFDAGNNDLTIKTMTTE
                     SGKNYMGVTIYMVSGSFIVNGNADNVMLEAPPDDPDPSPAIPGMLIYLAEGNDGLVKL
                     RGSSDSIFVGTIFAPDGDIDIAGNPDMPAGTDATFNTQLVAYNVKIGGNAFVDVNFDE
                     DTSYLMPTNLELHR"
BASE COUNT         2177 a         2139 c         2147 g         2012 t
ORIGIN      
        1 agggagatga tccaggccaa accagcccaa actcatccga tcctggccag cttaacccct
       61 cgtgcgataa agattgtatt ccaacaatag atcaagttgg ccctgagggt gaagcgaatc
      121 ccatgccgat tcatgaaaac gagacgcaca tgcatggtgt caaacgtgaa aactctgaag
      181 aggcaaatca tgctggtgaa aacgcgggcc gcaatagttt tgggcaagaa gcgagcgact
      241 ctacaggacg atttggaagc ggttccagtt ctccataatg caattttata caaccagcat
      301 tatgcggttg gcgtgattat tatattcccg aacaaaaaag gtcaataacc ccttcctgac
      361 aaaatcgtgg ggaggattta ttgcgcttaa aatagcttat aatatgagtt tgggaaaatc
      421 atgacaatac atttgagtat tgttggaaat ttgggcaacc ggtgatatat cctagtcaat
      481 ttctgacaat taattacaat atgtaacaac caaccatgca taacgtttat aaggatagct
      541 tggatgtccg actggaatga ggttgatgac attcaactgc taaaatttgc ccaagaaggc
      601 aatacagaag cattcggtgc gatctacgag cggttcgctg acaaagtata tcgctatctg
      661 aatgcacact tggggaacag tctggatgca gaggatatca ccgaagaggt attcatccga
      721 gtctggcggt cgttaccaga ctaccatgaa cgaggaacac catttctttc atatgttttt
      781 cggattgctc gcaatgcgct gattgatcat taccgtcgtg aacgacgaac ggcaggtcag
      841 ttgtcttcgg aagacgtgat gatcgctgat cacaagcccg gaccgggtga ggtcgtcagc
      901 cagcagatgg agcacaagga actacgcgaa gccttggatg atctgaaaga tgattaccgc
      961 aatgtattga tcatgcgatt tttgagtgat atgagtcctg aagagacagc tcatgcaatg
     1021 ggtcgttcgg ctggggctgt gagggtcttg caacatcgtg ccctggcagc cttgcggaca
     1081 aaatttgaga agagcaaagg taaacgggat ggccgaaaag cgcgaaggga ttgagcaaat
     1141 tctgcagact tgcctggagc tgatccgcag cggtcaagag acggtggatt ccgcgcttgc
     1201 tcgctatccc gaccatgcgg atgagttaag ctcgctcctg gaaacggcta cctggctgca
     1261 agagcgcaga gatgccctgg ctccacggcc cgagtttatt gccgcctctc ggcagcggtt
     1321 gctggcccgt atcgagcagg aaaaagctgt cagtacgcca aaatcgctac gtacagtggg
     1381 tgcaagcgtt tggaacacgc tcttatctct ctttgcccag aaacgctttg cttaccagtt
     1441 tgtcgttgcg acgatattat tggtattttt gatttcgaca accagtggtg tagccgccgc
     1501 agcccaacat tcaatcccag gggacacact ctatggggtt aagatgagcc ttgagcagat
     1561 ccaactggca ttgtccttca gcgaagcccg ccgcgctcaa tattatgtca catttgcaga
     1621 gcgcagattg gtggaggtac aaagcctggt tatcgaaggc cgttatgaat atttgcacaa
     1681 taccgtcgat caattttatt ttcaagcgaa tgaggcgtcc cgtttgttgc ggtcgctggg
     1741 tacgacaaac accttgctag ccagggaatt ggctaataaa ttaaggaatg tcgtagaagg
     1801 acaaatttcc cttatgcctg ttctctctca agcggctccc ccagatagtc gctccgaaat
     1861 cgaacggctg atgcaaatgg cagagctagt caagagcgag gcgcaaaata tcgaagctct
     1921 catgggaggc acaccactgc caaccataac gcctacgctg ataaccatta ctcccgttcc
     1981 cccgacgcca gtacccacca agacacctgt acctacaaca gcgccggtcg ttaccgttgc
     2041 accgttgtcg acacctttgt ctgcaaagac ggaggctgca cccaccctaa ctgttcgacc
     2101 ctcgcgcaca cccgtgccaa ccctgcaaaa tagcggcaaa aagccgacac ccacacctac
     2161 caaggccggc ccggccaacc caactcgcaa acccacgaaa gagaaaaaag aaccaccagg
     2221 tctagaaaat aggcctccaa agcctacgaa aaaatcatga actagcggct gacgatttca
     2281 gtcgaattgt attttaactg cctgataaca gaactgtaag cgtaattaat gcgctcatga
     2341 tgtaatatat gggtgtgcag cgtgacatgt cgcgtaagtg gctttttttg tacttattaa
     2401 acgcatattt acatgtggta attatatttt ctgcataatg cattgtaatt tgaggaaaag
     2461 ggtttctatg ctgggtattt tgcgcaaatc tcgacaagtg gaccgtggac agggcatggt
     2521 ggaattcgcc ctggtattgc cactgttgct cttggtcatt ttcggggtga tcgagactgg
     2581 taggttactt ttcgtttaca gtgtagtttt cacctccacg cgcgaagctg cgcggtacgg
     2641 ttcggcatct ggcctcctta caggagcctt gaatcagtac caggattgta atggcattcg
     2701 agcggctgcc aagcgtgtgg gcgtattcgc tggaatacag gatagcgata ttacgatcac
     2761 ttacgatcgc gtgacgggta caggtgtctc tcccttggga agttgcccca ttggcggctc
     2821 tggaccaaag cttaccctgg gtaatcgcgt aacggttcgg gtaacagtca actaccgtcc
     2881 tattgtccca attccaaatt ttccctcatt cccaatcacc tcaacctctt ctcgcaccat
     2941 cttgaaaaac atctctatcc agggcacacc ggttgtctcg tcatctaccc tgcctgtggt
     3001 ctacctttcg ccggctacct ttgatcaaat tgagggtgat gatggcaaca catcgattgt
     3061 gactgtggtg gctttgctaa gcggcggccc tgcggaacaa aatgtcactc tcaatttcag
     3121 cttgagcggc agcgccacca agggagttga ctacaccgta gacaagagca gtccagtgat
     3181 catcccctca ggccaatcat cgactgaaat aaaaatcacc gtcatcggtg atgatctcag
     3241 cgagttcaat gagacggttg tcttaaccat cgagtcaacc ataaatgccg agcgaggtga
     3301 cccgtctatc tttgagctgg ctatcctcga tgacgacagc gatccgctcg tcatatttgc
     3361 cccgccttat cccttgctgc ccattcagga aaatttgggg tttatcgact ttgaagtgct
     3421 cctggtgaat cctgatggaa gtccggcgac gtctggaaag gatgtggagg tgtctttcag
     3481 ccttgcaggt gacgcaaccg aagtaataga ttataaacct ccgccctcca cgtttattat
     3541 tcctgccggg tccagcagca aaacgcttcg gtttgatatc gtcgaggata ccatagacga
     3601 accagatgag atatttattg tgacgatggt atcgtttgta aacgccaatc ctggcgcaaa
     3661 taaggtgcaa acagcgacca tcaaagataa cgatgatcca ccctacgtct cgtttaccac
     3721 agatagccag atcgtgtccg aatcgatcgg cattgtattg atcacggctt ggctgcagga
     3781 tgcatcgggc gctgaggtca aatcgggctt gcccgtgagt gtagtgttta atgtagaagg
     3841 caccgcatcc aacgacgacg attacaccat atcatccagt cctctcgaat tttcagccgg
     3901 cagctcgcgc gcggatattg tggtctatgt tacgaatgat agcgtagttg aaaccgacga
     3961 gaccgttatc gtgaccatgg gcgcattgac taatgctcag caaggttctc cttccaaaca
     4021 gacggtaacc atcacaccca atcccaccgt cttcttcaca ctggcttccc aaagcgtaca
     4081 cgaaaacgtc ctgaaagcag acatcgaggt tcagcttcta ccagcacaac ctggtgatgt
     4141 atcggtagca tttagtctgg ctggaacagc aacccagggg aaagactaca cgctagagac
     4201 gaacagcccg ttggtgttct cagcagggca aaccaccggt acgatttcac ttatcttaga
     4261 aatggacaag ttggatgaag cggatgagac ggtacaggtg acgcttagct caccaagcgg
     4321 cggtgccgtt ttgggaacgc cgaaggtgca cttgctcacc ctgattgatg ataacccaca
     4381 gcctttcgtg tacttttcgt cttcggacca atcggtggat gaaaacgcag gggatgtgaa
     4441 tgtgctggta cagctcagcg ccgcctcgag tctgcccgtc acagtgccgc tcagcctggg
     4501 cgggagtgcg gcaaaaggta gtgattacat ctttaatcaa acctccgtgg tcatcccggc
     4561 tggcattacg agcacgttac tcaccctgca agtgatcgat gacatcctgc cagaaacatt
     4621 tgagacgatc gtgctcgcca tggagacccc aaccaacgct gtggtaggga ctcccggcac
     4681 gcatgccatt acgatcaatt ccaatgacct gcctacctgc aacatattca agagcactga
     4741 aattaccttg aatgctcctg gtatgtactt gagtttgcca ctatccacct ctgtatcaga
     4801 tacgttaata cttagccgat taacaatttc ctggccgtcc ggcgcgccga actctcccat
     4861 gtttgacaag gtgttattta ataatttcga agtgtacgac agcaaccatc cgcactcacc
     4921 agtaacagtc accaactgga atggtctccc ctccaaccgg cagcttaacc caaacccatc
     4981 tacgattaaa ctgacgttca cgcgtgcact cgatcctgga acttacacat tgaccctgtt
     5041 attccacaat gagaccaccg gtttcgactg tacaccaata caaagcagcg ccaccatccc
     5101 gcaaccataa atgagggaac ccgatgcgca catactattg gattccttcg ctgatcactt
     5161 tactcacttt cttggccctg ctgttgccag ctggcctcgc acatgccgat gtgagcagcg
     5221 ggacagtgat cactgtcaat tcagcagttg atgatgagct aaatggcaca tgttccctgc
     5281 gggaggccat ccaggccgcc aacacggaca gcgcggtgga cgcttgccca gcaggggacg
     5341 gcgcagatac catcctgctg ggggcagaga tctaccagtt aagcattaaa gggaccggtg
     5401 aagaatatgc ccagaccggg gacctggaca ttcgcgaaga cctggtcatt cgagggcagg
     5461 gcatgaattc gaccgttgta gatggcggcg gcatcgaccg tgtgtttcac atctattgga
     5521 ctgacccaca tcccgtggtg cactttgagc acttgactat ccagaatgga aatgtgacca
     5581 aagatttgta tgggggaggc ggcatcctca acgagtatgg cacaatcacc ttccaggcag
     5641 tatctcttga gaataaccat agcatctacg acggtgttgg atccgctgtg gggggcggga
     5701 tggataattt gggtaccgcg acgctaataa actcgtatgt cagtaacaac ggggctgatg
     5761 atggcggagg tatcttcaac tcgggcacac tctccatcta ccgctcgacc ttgaacaaca
     5821 acactgctac ccttgatgaa ggcggagcgc tggataatta cgggggtacc gtaacattag
     5881 aggatgtcac catcagcgga aatatagcct tatcaggctc tggcgggggc atcttcagcg
     5941 atggcacttt gttcatatct aatacgacaa tctccggtaa tactgccagc ataaatggcg
     6001 gtgcgttata caacgcatcg gatgccacat tgcagaacgt caccatcagc ggcaatagcg
     6061 cagcttccgg cgggggcatc tataatacag gtcacctgga tataaccaat agcaccatcg
     6121 tagaaaattc tgctatctcc gctgaaccac caaatggggc gaatattgat aacgaggata
     6181 cggtcacaat tgagaacacg ataatcgccc tagggcaaat tgcccctaat tgtgcggggt
     6241 tggcggggct gacttcgttg gggcacaacc tagaggatgc caatacctgt aatttggatc
     6301 acgccgatga tcatccgggg gtaggagacc catctataga tgtgctggcg aacagaggag
     6361 gccctacctg gactcacgcc ttgaattcgt cgagcccggc gatagacacg gggaatgacg
     6421 acacctgtcc atctacagac cagcgcggag ccaaccgtgc tggtgaatat tgtgatatcg
     6481 gcgcttacga gtacgctggt gagttttcca tcgaaaatgt tttcctacca ttgatcacga
     6541 gatagttgta agaacatatt cattctttat tattgataag tgtagaacag gagtaacctt
     6601 gaaagacaaa gcactgcgca gatactcaga gaaaggccaa agcctgaccg aatttgctat
     6661 cagccttctt atcctgttgc tgcttctggc aggggcagtg gatgcaggcc gggcattttt
     6721 taccttcatg tctctgcgcg atgcggttca ggaaggcgcc ctatggggat catacaatcc
     6781 cacggagacg gaactcatca aggaccgggt gaggaaagcc tctaatcttg tacaagcgat
     6841 tgccgatgac cccaacgcca ctttggacat agatgtagat attgacgatg ctgcttgcaa
     6901 cggaaacggc atcaccgtgc aggccacata cgagaatttt cccatcacta tgccatttct
     6961 aggtacgatc ataggcagtc agaaagtgcc gatcagcgcc tcggtgacgg ataccatcct
     7021 tgtcccacct tgcgaaaaat agtgatgact atgggaggtg tttaatgttg gatacatata
     7081 cacaggaaaa aggtcaggcg ctggtgttga tcaccctggc aatggtcgtc ttgctcgggt
     7141 tcaccgcctt ggcgatcgat ggaggcatgg tttattcaga ccgccgccac gcccagaacg
     7201 cagccgatgc ctcatccctg gcaggcggca gcgcggcagc gctccaactg gaaaatgatc
     7261 atattacgta tggaacctgg ggttgtaaca aaggcgacat attagatgct atggacgctg
     7321 gggaaggcgc cgccatcgac cgggcagatg ataatggctt ctcgatcgat gcgaacatca
     7381 ctgattatca cggcgtgatg accgattgcg gtagcgagcc ttatggaccc tgggaagata
     7441 aattcattga catccatacc catatttcaa ccaccacacc cacctccttt gcgcactttg
     7501 tctacggagg caagctgatg agccgggtcc attcagtgac ccgcatccgg ccgcgcatgg
     7561 ccttcgggtt tggcgcttcc atcctagccc tcaatgatga ccccaattgt gatggcaatc
     7621 aaaacggcgt cgtcttcgat ggcaacaatg aggttcaggt cgagggtggc gggattacat
     7681 ccaacgggtg cttaaagggc agcgggagca cactggatgt cgaagtggtc ggtggagata
     7741 ttatccacgt cggcgagatc gaaacacaac atgtggatac atttagcccc aacccggaat
     7801 ctggcaatgg cgtaaaattg ccagattttg cggtggctta tcctaagccg gattgctctg
     7861 aagtggacga ttttgatcat ccgagcactg agtttcgcaa taatgccggt gggacgtatt
     7921 tggatcctga attcatccct gccggtaact attcagcgat caagatgaat ggcacagtgg
     7981 tactggaggg cggtggtctg tattgcctgt acggtgattt cgatgcaggc aataacgacc
     8041 taaccatcaa gaccatgacc acagagtctg gcaagaatta tatgggcgtg acaatttata
     8101 tggtttcagg aagctttatc gttaatggca acgccgataa tgtgatgctc gaagctccac
     8161 ctgatgatcc cgatccatcg ccagccattc cgggtatgtt gatttacttg gcagaaggga
     8221 atgatgggct ggtgaagctg cgcggctcct cagactcgat ctttgtgggt acgatttttg
     8281 ccccggatgg cgatatcgac atcgccggta acccagacat gcctgctggt acagatgcca
     8341 ctttcaacac ccagctggtg gcctataatg taaaaatcgg tggtaatgca tttgtcgatg
     8401 tcaactttga tgaggatacg agctatctga tgcctaccaa cctggagttg caccgctgat
     8461 atagaatacc catac
//
LOCUS       DBNGSX010000306         3137 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_232753, whole genome shotgun sequence.
ACCESSION   DBNGSX010000306 DBNGSX010000000
VERSION     DBNGSX010000306.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_232753"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            521..901
                     /locus_tag="AC16CB_09275"
     CDS             521..901
                     /locus_tag="AC16CB_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342699.1"
                     /translation="MKPKLLLTLTAIYMGFIGLRTLISPAGTFGLDAGASAILIASFR
                     SIASLFIGSAVVNWFARSAEASKARDAIFLSNTVVFGLWTILWVLIILAGAPAFGWVF
                     VVIHLLFTVGFFVVGRANMSTNTT"
     gene            1189..2712
                     /locus_tag="AC16CB_09280"
     CDS             1189..2712
                     /locus_tag="AC16CB_09280"
                     /inference="COORDINATES: protein motif:HMM:NF033551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HIR3342700.1"
                     /translation="MAKFKPCNEDQMVMLPISLQDQLVPGTLEHTISQLVDQHVDLSV
                     FDGRYNNDETGATAIHPKILLKVILVAYSKGMISSRQIERACQENVIFIALSYGYAPD
                     HSTIAQFISSMQVEVQSIFSNILLVCEEMGLLGGTHFSLDGVKLSANVSKEWSGTFDE
                     LKHKRDKLQEKLQQVIAEHAQADKQPEVEIERQKKRERRLQVQVERLNEFLQTQEPKR
                     GSEGKEIQSNVIDNESVKMPSSHGVVQGYNAQALVDSKHQVILAAEAFSSQDHENLEP
                     MLAGAKKNVAAIGKDETYFQGKQLTADSNYHSYEALVVCKDEKVDAYIPDIQFRKRDP
                     RFADQQRFQGGIHPRQRPNAKPSQFSASDFSFDGNKQVYLCPHGKELTCHARNQTNRF
                     RIYDLYRARAQDCAVCPLRSRCLSKPTSSRRYLSVQVDTQPPNLIDEMKAKIDSEQGK
                     QIYARRLGIVEPVFANICVHKRMHRFTLRSKSKVDVQWRLFALVHNIGKIHTFGTIH"
     gene            2867..>3137
                     /locus_tag="AC16CB_09285"
     CDS             2867..>3137
                     /locus_tag="AC16CB_09285"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3342701.1"
                     /translation="MNKIPIKEGFIPFRGYKTWYRIAGDHEEPGKAPLMCLHGGPGFS
                     WDGFEPLEAIVDTGRRVIFFDQLGGGNSDDPHDPSIYTVGLYVEEV"
BASE COUNT          830 a          772 c          764 g          771 t
ORIGIN      
        1 attgccctat aatgaagtct tatcttctct gattcccgaa ttgaggtccg ataatataag
       61 aagagggtta tagatcttaa taacgcagat gctcgctggc aagcaatact aattcatgct
      121 gtccaaacgt aagcttgttc cgttcgatag gcataatgct tgaggcgtac agcgtcgcga
      181 acctagtcga gaagcttttt ggtttttgtg ccaaaggtca actttcgatc ctatttatgc
      241 tatacttttc tcagtatctt atcctatttt aatcttaagc aacctaaaaa aaggccgcta
      301 gtaacccata agttctagtg tgccaaaggc gtccgaaaca tgagttgggc ggtccaagga
      361 cacggtaaaa ggaggaacaa atgtgaatta ttgcccccca cagattgaag ttgaatctgg
      421 gcatccgtca agtaaaaatt tgatgccttg ccaccagaac tatttttgat tgtgctggaa
      481 taataagtaa ctttcttatt gttaaacagg agaaaatctc atgaaaccta aattgttact
      541 cactctcacg gccatctata tgggcttcat cgggctgcgc actctgatat caccagccgg
      601 gactttcggc cttgatgcag gggcatctgc tatattgata gcctcattcc gatcaatagc
      661 cagcctcttt attggctctg ctgtagtaaa ttggtttgct cgaagcgctg aggcttctaa
      721 ggcgcgcgat gcaatcttcc ttagcaacac ggtagtgttc ggcctttgga caatattgtg
      781 ggtacttatt atattggctg gtgcaccagc ttttggttgg gtgtttgttg tgatccacct
      841 gctctttaca gttggttttt tcgtggtggg ccgcgcaaat atgtcaacga ataccaccta
      901 gatgaacatt gtatatcctc catccgatgt cccatataac gggaatgtcg ggtggaggat
      961 gatttatatt cgatctaatg cccgcccaac acagcgtgca ccggattggt gggtgggctg
     1021 cgcgttataa agtagaattg tgcaaagttc gtggtttcgt tggtcgggaa cgttgtcccc
     1081 gccaaccagt aacgcaagcc gttgaaactg tcgaaaaagt ggggaaatat atcgcgcctg
     1141 gttaataact aatgcaaagc gatggggttt tataagacag aggagcttat ggcaaaattc
     1201 aaaccatgca atgaagatca gatggtcatg ctgccgattt cgctgcagga tcaactggtg
     1261 ccgggcacgc tcgagcacac catcagtcaa ttggtggacc agcatgttga cttgtcggtg
     1321 tttgatggac gatacaataa cgacgagacc ggcgctaccg ccatccatcc caagattctg
     1381 ctcaaggtca ttctggtggc gtactcgaag ggcatgatca gttcgcggca gatcgagcgc
     1441 gcctgccagg agaacgtcat tttcattgcg ctctcgtatg gctatgctcc cgatcacagt
     1501 acgattgcgc aattcatctc ttccatgcaa gttgaggtcc agagcatctt cagcaacatc
     1561 ctgctcgtgt gcgaggagat gggtctgctc ggcggcacgc acttcagcct cgatggcgtg
     1621 aagctctccg ccaatgtctc caaggagtgg agcggcacgt tcgacgaact caaacataaa
     1681 cgcgataagc tgcaggaaaa attacagcag gtgatcgctg aacatgcaca ggcggacaag
     1741 cagcccgaag tcgagatcga acgacaaaag aaacgcgaac gccgcctcca ggtccaggtc
     1801 gaacgcttga atgaattcct gcaaacgcag gaacccaaac gcggcagcga gggcaaggaa
     1861 atccaaagca atgtcattga taatgaatcg gtgaagatgc cctcttctca tggcgtcgtc
     1921 cagggataca acgcccaagc gctggttgat tccaaacatc aggtcattct cgccgccgag
     1981 gcgttcagca gccaggatca cgaaaatctc gagccgatgt tggctggtgc aaaaaaaaac
     2041 gtcgccgcca tcggaaaaga tgaaacctat tttcaaggca agcaactgac cgccgatagc
     2101 aactatcatt cgtatgaggc gctggttgtg tgcaaagatg aaaaggtcga cgcgtatatc
     2161 cccgacatcc aattccgtaa acgcgatccg cgctttgccg atcaacaacg cttccagggt
     2221 ggcatccacc ccaggcagcg acccaatgca aaaccgagcc agttcagcgc ttctgatttt
     2281 tcttttgacg gaaacaaaca agtgtatctc tgtccgcatg ggaaagaact gacctgccat
     2341 gcccgcaatc agaccaaccg ttttcgcatc tatgatctgt atcgcgcccg cgcacaagac
     2401 tgcgccgtct gtcctttgcg atcgcgctgt ttgagcaaac caacgtcatc gcgcaggtat
     2461 ctttccgtcc aagtggatac gcagccgccg aacctgatcg atgagatgaa ggcaaagatt
     2521 gactctgaac agggaaagca gatctatgcc agacggctcg gcattgtcga gccggtcttt
     2581 gccaacatct gtgtccacaa gcgcatgcat cgttttacct tacgctccaa atccaaagtg
     2641 gatgtacaat ggaggttgtt cgctttagtc cacaatatcg gcaagatcca cacttttggg
     2701 acgatccact gacccctgac agagcaaaac acgccttcga cgcgagaaac tggcctctcg
     2761 atttgcccct ggacaggaaa acctctctaa aaaaccagtt tttcgacagt ctcgttagcc
     2821 cgctccatgg aaagcgagac aagtatcaag tatcaagaag gtgacaatga acaaaatacc
     2881 aatcaaagaa ggctttatac catttcgtgg ctataaaacc tggtatcgca ttgctggaga
     2941 tcacgaagaa cctggtaaag caccgcttat gtgtctgcac ggaggtcctg gattctcttg
     3001 ggatggcttt gaacccctag aagctattgt ggatactgga cggcgtgtga tcttctttga
     3061 ccaacttggc ggtggcaact cagatgatcc acatgatcca tcaatatata ctgtcggcct
     3121 ctacgttgaa gaagtcg
//
LOCUS       DBNGSX010000307         2142 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1756971, whole genome shotgun sequence.
ACCESSION   DBNGSX010000307 DBNGSX010000000
VERSION     DBNGSX010000307.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1756971"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="AC16CB_09290"
     CDS             complement(<1..1230)
                     /locus_tag="AC16CB_09290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR3342702.1"
                     /translation="MLMNIVPDQKSRRTLVFDLLLVCVLVIGAYFRLVGLNWGEYQYL
                     HPDERFLIWVGSDIQPVGTDPEKLGPPPSTALNPARGNHPDAFPDCQVWGGYFDASCS
                     PLNPNNRGHGFYVYGTLPMFLTRYIVEWIYGHSGFQEMTDVGRFLSAMADLLTVFLVY
                     VIATRLYDRRVGVLSAAFSAVAVLQIQLSHYFAMETFLNLFTYLAIYFAVRVGDTKWQ
                     VGEEGVLAAGEMTGITESAKRFVKHPLFIFSIAFGFALGCAVASKLNAGAVAVALPVV
                     FGLKLLAVPAEERMRRLGEAFAYLCLAAVVSLLAFRLFQPYAFSGPGFFGLKPNPMWV
                     SNILEQRIQASGDVDFPPALQWARRPVWFSGKNLVLWGLGVPLGLLAWAGFLWAGWKL
                     LADKSENHKELRRHGLLW"
     gene            complement(1303..>2142)
                     /locus_tag="AC16CB_09295"
     CDS             complement(1303..>2142)
                     /locus_tag="AC16CB_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342703.1"
                     /translation="RLGTIEQPLPGPAGQIFLGNLWKALSMFAWDNGEIWAISVTHRP
                     ALDVVSAVLFYLGSVFVLLRYLRRRQWPDLFWLLAVPLLMLPSILSLAFPAENPALNR
                     LSGAIVPVFLIVGLALDGFLETLKSQFGGRLGNALAVGAGLFLVIWASFQNFDIAFNQ
                     YQETYLRSSWNTSELGQVIHDFAGGSGTPDTAWVIAYPHWVDTRLVAMNAGFPTRDYG
                     IWPENLQETLPETRAKLFLLKPEDEVGLSTLRQLYPDGIQSVYPSKVEGHDFLIYFVP
                     PLK"
BASE COUNT          617 a          611 c          511 g          403 t
ORIGIN      
        1 ccacaacaag ccatggcggc gaagctcctt gtgattctcg ctcttatctg ctaacaattt
       61 ccaaccagcc cacaggaagc cagcccatgc caacaacccc aaaggcacgc cgagccccca
      121 aagcaccagg tttttccctg agaaccatac agggcggcgc gcccactgca aggctggagg
      181 gaaatccaca tcccctgatg cctggattcg ctgctcgaga atattactaa cccacatcgg
      241 attgggcttc aacccgaaga aacccggacc agaaaaggca tagggctgga acaggcggaa
      301 ggcaagcaag ctgaccacag ccgccaaaca tagatacgca aaagcctctc caaggcggcg
      361 catgcgctct tcggctggga ctgccagaag cttcagccca aatactaccg gcagagcaac
      421 cgccacagct ccagcattca gcttggaagc cacagcgcag cctaaagcga aaccgaaggc
      481 tatgctgaag atgaaaagag ggtgcttcac aaaacgcttt gcggactctg ttatgcccgt
      541 catttcccct gctgccagca caccttcctc tccaacctgc catttcgtat ctccaacccg
      601 taccgcaaaa tagatcgcca gataagtgaa caggttgaga aatgtctcca tggcgaagta
      661 atgcgagagt tggatctgca gcactgccac tgcactgaaa gctgctgaca gaacacccac
      721 acgccggtca tacagtcgag tagcgatgac atataccaga aaaacggtga gcagatcggc
      781 catagccgac aagaagcggc ctacgtcggt catctcctga aatcctgagt gcccataaat
      841 ccattcaacg atgtagcgcg tcaggaacat cggcaatgtc ccgtagacat agaaaccgtg
      901 gccgcggtta ttcgggttca agggagaaca ggatgcatca aaatacccgc cccagacctg
      961 acaatcaggg aatgcatcag ggtggttgcc tctggctgga ttgagagcag tagaaggtgg
     1021 aggacctaat ttctcgggat ccgtacccac aggctgaata tccgaaccaa cccaaatcag
     1081 gaagcgctcg tctgggtgta aatactggta ttcaccccag ttcagtccga cgagacggaa
     1141 atacgcccca atgactaaga cacaaaccag cagtaaatcg aaaaccagcg ttcggcgtga
     1201 tttctgatca gggacaatgt tcataagcat ctatggcatg atgtgcggca cagtttgcaa
     1261 taatcaattc ttccatacca tctactcgcc acacattacg cctcacttga gcggtggcac
     1321 aaagtatatc aagaagtcat gcccttccac cttggaaggg taaacacttt ggattccgtc
     1381 aggatagagc tggcgcaatg tgctcagccc aacctcatcc tctggtttta gcaagaatag
     1441 cttagcgcgt gtctccggga gggtctcctg cagattctcg ggccagatcc cataatcacg
     1501 ggtcggaaat ccggcattca ttgcaaccag gcgagtgtct acccaatggg ggtaagcgat
     1561 cacccaggcg gtatccggag tgcctgaccc gccagcaaaa tcatggatga cctgcccaag
     1621 ctccgacgta ttccacgaac tgcggagata tgtttcctga tactggttaa acgcaatgtc
     1681 gaaattctga aacgatgccc agatgaccag gaaaagaccc gcacccacag caagtgcatt
     1741 tcctaacctg cctccaaact gagacttgag cgtctctaaa aaaccatcta aggccagtcc
     1801 cacaatgagg aaaaccggca cgatggcacc agacaatcga ttaagtgccg gattctctgc
     1861 tgggaaagcc aaagacaata ttgatggtag catcaacaaa gggactgcta gcagccaaaa
     1921 aagatccggc cattgtcgcc gccgcagata gcgaaggaga acaaaaaccg acccgaggta
     1981 gaaaagtaca gctgatacca cgtctaaagc aggtcggtga gtaacagaga tcgcccaaat
     2041 ttccccatta tcccaagcaa acatgctcaa cgccttccac aggttcccca aaaaaatctg
     2101 cccagctgga ccaggtagtg gttgctcaat cgttcctagg cg
//
LOCUS       DBNGSX010000308         3976 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1826047, whole genome shotgun sequence.
ACCESSION   DBNGSX010000308 DBNGSX010000000
VERSION     DBNGSX010000308.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1826047"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="AC16CB_09300"
     CDS             <1..298
                     /locus_tag="AC16CB_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342704.1"
                     /translation="EDDVAAGLRKLFSYKDKYYGDLYNPLHTSRLRVEKVDFDKGGLI
                     QVWFSGTYKPSGDPCDNTRVKAQIWNTIKQFRGVKKTNIFLNGIPFGDRLSNDK"
     gene            318..1034
                     /locus_tag="AC16CB_09305"
     CDS             318..1034
                     /locus_tag="AC16CB_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342705.1"
                     /translation="MNLPDFSTIPWRLAVWRLAIIIAFGLAAGCAALPTPAAVSLPTA
                     SSSPSLPTPTSQPPTATVPPPTETATAIPHTSTPTVTPLPSETPAPTETPTETSPPPT
                     PSGEDAIYVYYILLNDGGPVACGDSLVKANTGLARTGDIEADVRTALERLFFKRQYFG
                     RLYNPVYLSNLEVESVKYDKNTKEVSVRLDGTYVRSGDRCDDGRVRAQIWTTIRQFEG
                     VKTVYILLRESLLGDILATR"
     gene            complement(1163..3931)
                     /gene="ppdK"
                     /locus_tag="AC16CB_09310"
     CDS             complement(1163..3931)
                     /gene="ppdK"
                     /locus_tag="AC16CB_09310"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HIR3342706.1"
                     /translation="MADHKWVYLFDEVEQAEKHVGGSWEGVRALLGGKGSGLADMTRA
                     SVPVPPGFTVTTEACNAYLEAGQKFPEGMWEQELEALKQVEAQTGKKFGDPTRPLLVS
                     CRSGAKFSMPGMMDTVLNIGLNDEVAEGMAKLTGDERFAFDAYRRLVEMFGEVVLGIP
                     DEKFEHPMAEYKEKKGVKADTELTAEDWKAITETFKKVVRENRGFDFPQDPIEQLRLA
                     TEAVFKSWNGKRAVDYRNAAGIAHDLGTAVNIVTMVFGNMGWNSGTGVAFTRDPANGE
                     KKMYGDYLLNAQGEDVVAGIRNTEMITNLANVLPEAYQQFLEICDKLELHYKEMQDVE
                     FTIEQGKLWMLQTRSGKRTAKAAVKIAVDMANEGLISKQEAILRVTPDNVDTLLHPQF
                     DEAAKKSAAVDGRLYATGVNASPGAAVGQVYFDADTAEHMAKENKQDVIMVRPFTKPD
                     DVHGMLASKGILTSEGGATSHAAVVARQFGVPCVVGAAMVRINLDKREMVVNGQVVRE
                     GEWISVDGTTGEVFLGHIPTTSPTLEEQTDLLTLLHWADEICATPGIRSAPDGWPTTG
                     LQVWANADYPKDARRARSYGAVGIGLCRTEHMFFEPERLPIVQRMILAETSEARKAAL
                     DELLPSQRTDFEGLFEAMDGYPVIIRLIDPPLHEFLPSEEDLLEEVITMRVKGENKGL
                     AEKEKLLASVKSMHESNPMMGLRGVRLSIQMPEIVEMQVRAIFEAAANMTIKGVVVKP
                     EVMIPLTGTINELRWIQPRLEKIAKAVMDEKQVTFSYKFGTMIEIPRAAVTATEIAEL
                     AEFFSFGTNDLTQMTFGYSRDDAERNFLVTYVEQGILSNNPFQTLDRGGVGRLMDLAV
                     KDGRQTRPDLEVGICGEHGGDPSSIEFCHTIGNNYVSCSPFRVPIARLAAAHSALKHK
                     GVSIAEDK"
BASE COUNT          897 a         1093 c         1001 g          985 t
ORIGIN      
        1 tgaagatgac gttgcggcag gtttgagaaa gttgttctca tacaaggata agtattacgg
       61 cgatttatac aatccgctgc acacctccag gctgcgcgtc gagaaagtgg attttgataa
      121 aggaggcctg attcaagttt ggttcagtgg tacctataaa ccctcaggcg acccatgcga
      181 taatacccgc gtcaaggcac agatctggaa cacgatcaag cagtttcgag gagtaaagaa
      241 aacaaacatc ttcctgaatg gcatcccctt tggagatcgc ctatcgaatg ataaataggc
      301 tggatagtag attatttatg aatcttcctg atttttccac cataccctgg cgcttggcgg
      361 tgtggagatt ggcaatcatc attgcatttg ggctggctgc gggttgtgcc gcgctgccaa
      421 ccccggctgc ggtctctttg cccacagctt cttcatcccc ctctctacca acaccgacat
      481 cccaacctcc cacggcaacg gtccccccac ctacggagac agcaaccgcg attccgcata
      541 catctactcc tacagttaca ccgttgcctt cagagacacc agctccaacg gaaactccta
      601 ccgagacctc tcctcctccg acgccgtctg gcgaagacgc gatctatgtc tattacattc
      661 tattaaacga cggtggtcca gtggcttgcg gtgacagcct ggtgaaagca aataccggtc
      721 tggcgaggac aggtgatatc gaagcggatg tgcgtaccgc tctggagaga ttgtttttca
      781 aacgccaata ttttggtagg ttatacaacc cggtttatct ctccaacctc gaggtggaga
      841 gtgtgaaata tgataagaat accaaagagg tttctgtgcg cctggatggc acctatgttc
      901 gctcaggtga tcggtgtgac gatgggcgtg tgcgcgctca aatttggacc accatccggc
      961 agtttgaggg ggtcaaaacc gtgtacatct tgctgaggga gagcttgttg ggcgatatcc
     1021 tggcaacaag gtaaccgttt ccattcctgc gactacgtaa cgcattaaat aaataatggg
     1081 atcgcgcaag cggcagttag ccagcgcgat cccattaact ttaaaggatt ttctcgctcg
     1141 gaggaaagag gattggtgct acctacttat cctcggcaat agacacacct ttatgcttca
     1201 gtgcggaatg ggcagctgct aatcgagcaa tcggcacccg gaagggagag cagctcacat
     1261 agttattgcc aatcgtgtga caaaactcaa tagagcttgg atcgccgcca tgttcgccgc
     1321 agatacccac ctctaagtca ggtcgggtct ggcgtccgtc tttgacagcc aaatccatca
     1381 atcgtcctac tccacctcga tccagggtct ggaaggggtt attggacaag attccctgtt
     1441 cgacataagt cacaaggaag ttgcgttcgg cgtcatctcg gctatagcca aaggtcattt
     1501 gggttaagtc gttggtgcca aaggagaaga actcagctaa ctcggcgatt tcagtagctg
     1561 taaccgcggc acgggggatt tcgatcatgg tgccgaactt ataggaaaag gtgacctgtt
     1621 tctcatccat aacagccttg gcgatcttct ccaggcgggg ttggatccag cgcagctcat
     1681 tgatcgttcc agtcaatggg atcatcactt ccggtttaac taccactccc ttgatagtca
     1741 tgttggctgc tgcctcgaaa atggcacgca cttgcatttc gacaatctct ggcatctgga
     1801 tgctcaagcg cacaccgcgc agacccatca tggggttact ttcgtgcatg gatttgacac
     1861 tggcgagcaa tttctctttc tctgccagtc ccttgttttc acccttaact cgcatggtga
     1921 tgacctcttc gagtaaatct tcttccgagg gtaaaaattc atgcagtggg gggtcaatca
     1981 ggcggatgat gaccgggtag ccatccatgg cctcaaaaag accctcgaag tcggtgcgtt
     2041 gggacggaag aagttcgtcc agggcagctt tgcgcgcctc actggtttca gcgaggatca
     2101 tgcgctgcac aatcggcagg cgttctggtt caaagaacat atgctcagtg cggcacagcc
     2161 caatgccgac tgcaccgtat gagcgggcgc ggcgggcatc cttgggataa tcggcgttgg
     2221 cccaaacttg caagccagtc gtgggccagc catcaggggc ggagcggatg ccaggtgtgg
     2281 cgcatatctc gtcagcccag tggagaagcg tgagtagatc ggtctgctcc tccaaggtgg
     2341 gagatgtggt ggggatatgc ccaaggaaca cctcgccagt cgtgccatct acagagatcc
     2401 attcaccctc gcgcacgacc tgcccattaa ccaccatctc gcgcttatcc aggttgatgc
     2461 ggaccattgc agccccaacc acacatggca cgccaaattg tcgcgccacc actgcggcgt
     2521 gggaggtagc cccaccttca ctggtaagga tgcccttgga agctagcatg ccatgcacat
     2581 cgtctggctt ggtgaatggc cgtaccatga tgacatcctg tttattctct tttgccatgt
     2641 gttcagcggt gtcggcatcg aagtacacct ggcctactgc agccccaggt gaagcattca
     2701 cacccgtggc atacaggcgc ccatccactg cggctgattt tttggctgct tcgtcgaact
     2761 ggggatgaag caaggtatcc acgttgtctg gggtgacgcg caggatagcc tcctgttttg
     2821 aaatcagccc ctcgtttgcc atgtctacag caattttgac ggcggctttt gcagtacgtt
     2881 ttccgcttcg cgtctggagc atccacaact ttccttgctc gatggtgaac tccacgtcct
     2941 gcatttcttt ataatgcagc tccagcttgt cgcagatctc caaaaattgc tggtacgctt
     3001 ctggcaaaac attggctaaa ttcgtaatca tttcagtatt gcgaatgccc gcaaccacat
     3061 cctcaccttg agcgttgagc aaatagtcac catacatctt cttttcacca ttggctggat
     3121 cgcgagtgaa cgccacaccc gtgcccgaat tccagcccat atttccaaaa accatggtga
     3181 cgatattaac cgcggtgccc aagtcgtggg ctatgccagc tgcgttgcgg taatccacgg
     3241 cgcgcttgcc attccaagat ttaaagacgg cttcggtcgc caggcgtagc tgctcgatag
     3301 gatcctgggg aaagtcaaag cccctatttt cgcgcaccac cttcttaaag gtttcggtga
     3361 tggctttcca atcctcggca gtcagttccg tatcggcttt tacgcccttt ttctctttat
     3421 attccgccat gggatgttca aacttctcgt cgggaattcc gagaaccact tcaccaaaca
     3481 tttccaccaa gcgacggtag gcgtcgaagg caaaacgctc gtctccggtt agcttcgcca
     3541 tcccctctgc tacctcgtcg tttagtccaa tgttaagaac tgtgtccatc atgcccggca
     3601 tggagaattt tgctcccgag cggcatgaga ccaatagggg acgggtgggg tctccgaact
     3661 tcttgccggt ttgagcttcc acttgcttca acgcttctag ctcctgctcc cacatgcctt
     3721 cgggaaattt ttggcctgcc tccagatagg cgttacaggc ctctgttgtg accgtgaaac
     3781 cgggcgggac gggtacgctg gcacgcgtca tatctgccaa cccggagcct ttgcctccga
     3841 gaagcgcgcg cacgccctcc cagctacctc ctacatgctt ttctgcttgt tcgacctcat
     3901 caaataggta aacccattta tgatcagcca tgaaaaaaaa cctcctaggt aattcattat
     3961 ttacgggata atgccg
//
LOCUS       DBNGSX010000309         4045 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1410647, whole genome shotgun sequence.
ACCESSION   DBNGSX010000309 DBNGSX010000000
VERSION     DBNGSX010000309.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1410647"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(314..928)
                     /locus_tag="AC16CB_09315"
     CDS             complement(314..928)
                     /locus_tag="AC16CB_09315"
                     /inference="COORDINATES: protein motif:HMM:NF017959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HIR3342707.1"
                     /translation="MNQKVLYFCGMLVPFTYIFMYILGGALRPGYSHISDSVSELLSR
                     GAPNKKLLDSFNVAFALFYILFGFGVFQFVMGSDHNALVGRIGAGMIIAVGIASIGSA
                     IFPQDATDTPRTLPGTLHLIFVFGVQIPGAIISTLLIGIWMNQADILSGFATYSFISA
                     GAIVLSGALAGPSMGKPFMGVVERISALAVHQWIFFLAWNLLGS"
     gene            complement(942..1778)
                     /locus_tag="AC16CB_09320"
     CDS             complement(942..1778)
                     /locus_tag="AC16CB_09320"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR3342708.1"
                     /translation="MKAFVNRYQISIFFILTFVISWFPWYAGIAPEVMAMGPSIAAFT
                     IALIVGGRRGFVDLLRPFGRWRASLGVWGLAIFGPGILYLIGLVVHLLMGGEAPPFIM
                     IREELNLLLLYLFLVVLMPWNGPVGEEFGWRGYVLPKLQNKYSPLIASLVIGTVWGIW
                     HLPSFFAPQGVISALTTALGIGFLIPYSLGTIANSVFMAWLYNKSKASALIAGIIWHA
                     AIDFWAPILLSDSSLVAAREGTHLPTIAPNLYLTVLAVQVVVASVLVIATKGKLGFSE
                     QT"
     gene            complement(1775..2491)
                     /locus_tag="AC16CB_09325"
     CDS             complement(1775..2491)
                     /locus_tag="AC16CB_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HIR3342709.1"
                     /translation="MNTHRRTAILVGIFFIIGTVAGVSSGVVTGPVLGGSDYLAKVAA
                     NESKIALGALLVLVMGFPLAMIPVLMFPIFRKYSEPLALGAVVFRGVLEAVTYMALAV
                     YWLLLIPVSQEFVKAGTADALTFQAMGAFLTKAGYWTEHLLALVFTIGAMMLYWLFYK
                     TKLVPNWLSLWGFIGAILNFVAAIFNMFDPTHPSLSLGVKWGNLIIPLAIQEMVFALW
                     VIVKGFNPDAAILKDRIVAS"
     gene            complement(2684..3130)
                     /locus_tag="AC16CB_09330"
     CDS             complement(2684..3130)
                     /locus_tag="AC16CB_09330"
                     /inference="COORDINATES: protein motif:HMM:NF039408.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6326 family protein"
                     /protein_id="HIR3342710.1"
                     /translation="MNTKQTASMLEDRKVNVKAKLALLWVALMFLYIYNDLLSLMQPG
                     HVAQLVEGHLEGVHFTQTLLFSAAVLMAFPGFMILLSLILKAKANRLVNIVIGIFHIL
                     VLLGTQFVGEGETWFYWRLYELLEALFLALIIWTAWKWPTTESQHV"
     gene            complement(3141..>4045)
                     /locus_tag="AC16CB_09335"
     CDS             complement(3141..>4045)
                     /locus_tag="AC16CB_09335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIR3342711.1"
                     /translation="KENEVLIKVFATTVTKYDCWMRSCTAPPGFGLLMRISSGRKPKQ
                     PILGTELAGDIDSVGKDVTRLKVGDQVFGYPGMNLGAYAEYICLPEEAVAIKPANVTY
                     EEAAAVLQGALTALFFLRRGHIQRGQKVLIFGASGGVGSYAVQLAKHHFGTEVTGVCS
                     TSKLEFVKSLGADKVIDYTKQDFTQNGQVYDTIFDTVGMTSVSRSKRSLRENGSYLIA
                     TFGLPMLLQLLWLSKTGSQNLEFGVLKEKTEDLIFLKDLIEAEVIKPVIDRCYPLDQT
                     VEAHRYVETGHKKGNVVITVEHDK"
BASE COUNT         1126 a         1025 c          891 g         1003 t
ORIGIN      
        1 tacacctttc aaactttgca ggagtagcct ccagggccac tctggctgtt gacctcccct
       61 tacatctttt tcagccaaca aagtttcact tactacttga atctacgtag ttattattga
      121 attgaaccca aataaaactg tggtccctgt ctacatcctt gatgtgatta ctcaatcgca
      181 ggcaattcac tctgttggtg cctgcgcaaa agtctctggg gaaattgcag ttgggtcaaa
      241 tcccttcacg gtcaaccaca gcgcgaatac catttcttga atagccaaca gagttatcaa
      301 tatatctccg ttgctagctt cccaacagat tccaagccaa aaaaaatatc cattgatgga
      361 cagccaaggc agagattcgt tcaactacac ccatgaatgg cttccccatg gatgggcccg
      421 caagtgcacc tgaaagaaca atagcacctg ctgaaataaa cgaataggtt gcaaaccctg
      481 agagaatatc cgcttgattc atccagattc ctatcaacaa cgttgaaatt atggcgccgg
      541 ggatttgaac gccaaacaca aatatcaagt gcagggttcc aggcaaagtg cgtggagtgt
      601 ctgtcgcatc ctgaggaaag atggcactcc cgatactggc tatcccaaca gcaatgatca
      661 tcccagctcc gatacgacct accaatgcat tatgatcact tcccattaca aactggaata
      721 ctccaaatcc aaacaatatg taaaacaagg caaaggctac attgaaacta tccagtagtt
      781 tcttgttggg cgctccccgc gaaagtaatt cactcactga atccgaaatg tggctgtaac
      841 ctgggcgtaa tgccccaccg aggatgtaca tgaagatata agtgaatggc acaagcattc
      901 cacaaaagta caaaactttc tggttcatta tgtttccatt atcaagtttg ctctgaaaag
      961 cccaatttcc cttttgtggc aattaccaag acggaagcca ccacgacttg aacagccaaa
     1021 accgttagat aaaggttggg tgcgatggtg ggaagatgag ttccttcacg agcagcgaca
     1081 agggatgagt cgcttagaag tataggcgcc caaaagtcga tcgctgcatg ccaaatgatt
     1141 ccggcaatca gcgcgctggc tttggatttg ttgtacagcc aggccatgaa aacagaattg
     1201 gcgatcgttc ccaggctata agggataaga aaaccgattc ccaatgctgt tgtgagcgcg
     1261 ctgatcacac cctgaggggc aaagaaactg ggcagatgcc aaattcccca gacggtgccg
     1321 attaccaggc tggcaatcaa tgggctgtat ttattctgca atttgggaag cacatagcca
     1381 cgccagccga attcttcgcc cactggccca ttccaaggca tcaaaacaac aaggaaaaga
     1441 tatagtagga gcaggtttag ttcttcgcga atcatgatga acgggggagc ttcgccaccc
     1501 atgagcaagt gcacaacgag cccaattagg tataaaatcc ctggtccaaa gatagctagc
     1561 ccccatacac ccaggctggc tcgccagcgt ccgaacggtc gcagaagatc gacaaatccg
     1621 cgcctgccgc cgacaatgag cgctatggtg aaggcggcga tggatggacc catcgccatg
     1681 acttccggtg cgattcccgc ataccaggga aaccaactga taacaaaggt gaggatgaaa
     1741 aatatggaga tttgatatcg gttgacgaaa gccttcatga tgcgacaatc ctgtccttca
     1801 agatagctgc atctggattg aatcccttca cgatcaccca cagcgcaaat accatttcct
     1861 gaattgccag cgggataatc aagttgcccc atttgacgcc tagggaaaga gacgggtgtg
     1921 ttgggtcaaa catattgaaa attgccgcga cgaaattcaa gatcgctcct atgaaacccc
     1981 ataacgacag ccaatttgga acaagtttgg ttttataaaa gagccaatac aacatcatag
     2041 cgccgatggt gaaaaccaac gccagcaggt gctcggtcca gtaaccagct ttggtgagaa
     2101 aagcgcccat tgcctgaaaa gtcagtgcat ccgctgtgcc tgcctttacg aattcctggc
     2161 ttaccgggat gagcaaaagc caatagacag cgagcgccat ataggtcacg gcttccaata
     2221 cgcccctaaa aacaactgcg ccaagcgcca agggttcgct gtatttgcga aagatcggga
     2281 acatcaggac cggaatcatc gccaggggga aacccatcac caacaccagg agggcgccca
     2341 gggcgatttt gctctcattc gctgcgactt tggcaaggta atcagaacca cccaaaaccg
     2401 gaccggtaac aacgccgctc gaaacgccgg caactgttcc aatgatgaag aatatgccta
     2461 caagtattgc tgtccttctg tgtgtgttca ttttattttc tcctttcagg tagtcccttt
     2521 ttgattttct gaactacctt ctatgtaact actgcaggga ttaggccttt ctctctactt
     2581 ctgagcagtt acccttccat aactaatttt gtgaattaaa tgacacccta aaatgttcgc
     2641 aactgctcag gctaaggact ggattccacg atctggtaat agattacaca tgctgggatt
     2701 cagtagtagg ccacttccat gctgtccaga tgatgagcgc aaggaatagg gcttccagca
     2761 gctcatagag cctccaatag aaccaagtct ctccctcgcc cacaaactgc gtgccgagta
     2821 ggacaaggat atggaaaatg cctatgacga tattcaccaa gcgattcgct ttggccttca
     2881 atataaggga cagtagaatc atgaagcccg gaaacgccat tagcactgct gcactgaaaa
     2941 ggagtgtttg agtgaaatgt acgccttcca gatgtccctc taccagttgt gctacgtgac
     3001 ctggctgcat aagtgataat agatcgttat aaatatagag gaacatcaag gctacccata
     3061 gtaaagcgag cttggccttc acattgacct ttctatcctc tagcatgctt gcggtttgtt
     3121 tcgtgttcat ttttctcctt ttatttgtca tgttccacag tgatgacaac attccctttt
     3181 ttgtgtcctg tttcgacata gcgatgcgct tcgacagtct gatccagcgg atagcatcta
     3241 tctataaccg gtttaatcac ttccgcttca attaaatctt tcaggaaaat caaatcttcg
     3301 gttttctctt tgagtacccc aaattccaaa ttttggctac ccgtctttga gagccatagc
     3361 aattgaagga gcattggtag cccaaaggta gctataagat aggatccatt ttccctcagc
     3421 gatcttttgc tgcgcgaaac agaagtcatg ccaactgtat caaaaatggt gtcgtagact
     3481 tggccgtttt gagtgaaatc ctgtttcgtg taatcaatga ccttgtcggc tcccagagat
     3541 ttcacaaact ctagttttga ggtgctacag acccccgtaa cctctgtccc aaagtgatgc
     3601 tttgcgagtt gtaccgcata gctaccgacg cctccagaag cgccgaagat aagaaccttt
     3661 tgaccgcgct ggatatgccc tcttcgaaga aaaaacaatg cggttaatgc cccctgaagg
     3721 acggcggcgg cttcttcata ggtcacattg gctggtttta ttgccacggc ttcttcaggc
     3781 agacaaatgt actcggcata agcacctaaa ttcatccccg ggtagccaaa tacctgatca
     3841 cctaccttca atcgggtaac atctttgcca accgagtcaa tatccccggc tagctcagtg
     3901 cccagtatgg gttgctttgg ttttctccct gatgatattc gcatcaatag cccgaaaccg
     3961 ggcggcgcgg tacaactccg catccaacag tcgtatttgg ttacagttgt agcaaatact
     4021 ttgatcagta cctcattctc cttgg
//
LOCUS       DBNGSX010000310        14943 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1826151, whole genome shotgun sequence.
ACCESSION   DBNGSX010000310 DBNGSX010000000
VERSION     DBNGSX010000310.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14943)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14943)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14943
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1826151"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..688)
                     /locus_tag="AC16CB_09340"
     CDS             complement(95..688)
                     /locus_tag="AC16CB_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR3342712.1"
                     /translation="MERPKQDFNTLTLVTIPVAIAINIAIGQLIITLKLPIYLDSIGT
                     VLVGVLAGPWAGALTGLLSNLIWGLTGLNVTYAPFATVAAVIGALAGLFSQSGWLRAW
                     WKVVLAGLITGLVASALSAPISAYVFGGVTGAGTDVVVAIFRGMGWGILPSTFAQGAI
                     SDPIDKVVTFLIVYLIVSALPARFVFRFPGASNVKSS"
     gene            986..2941
                     /locus_tag="AC16CB_09345"
     CDS             986..2941
                     /locus_tag="AC16CB_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3342713.1"
                     /translation="MSNQLEFGGNFVWEPTPEYVERAHLTQFMGQHQINDFNELMHRS
                     TQDVAWFTDALLHYLDIRFYQPYTQVLDLSRGIAWPKWCIDGQMNIIHNCLDKYIGTE
                     AEERTALIWEGEEGVVRRLTYKELYRQVNRTANALRSLGLGKGDAIGLYMPMTPEIVI
                     ALLAIAKIGGVILPLFSGFGAGAVASRLADAEAKALFTADGFFRRAKPVSMKVVADEA
                     ARMVSSLQHLIVVNRAEIDVPMTAGRDHWWHELVDSQSDQADTEVTSAEDVLMVIYTS
                     GTTGRPKGAVHTHCGFPVKAAQDMAFGTDLHAGEVLYWVTDMGWMMGPWLAFGTLILG
                     GTMLLYDGAPDYPGPNRLWALVERHRITGLGVSPTLVRSLIPRGEEHFLKHDLSSLRL
                     FASTGEAWNPDPWNWLFEKVGRTRLPVINYSGGTELSGGIVMGNPILPLKPCAFSAPC
                     PGIAADVLDENGNPVRNQVGELVIKTPWIGITRGFWKDPQRYEETYWSRWPGIWVHGD
                     FALIDDDDMWYILGRSDDTIKVAGKRLGPAEIESILVSHPDVVEAAAIGVPDEIKGSE
                     VVAFAVLSTDCDPCEELRQELYKMIVKALGKPFAPREVLFVSDLPKTRNAKVMRRMIR
                     AAYLGEDPGDTSSLVNPEAVEEIRVAR"
     gene            2954..3436
                     /locus_tag="AC16CB_09350"
     CDS             2954..3436
                     /locus_tag="AC16CB_09350"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR3342714.1"
                     /translation="MSDKAFQDFYSEQSSHCYGCGRLNELGLQIKSYWDGDETVCVFQ
                     PRPYHTAIPGYVYGGLIASLIDCHSTGSAVAAAYRAAGRSMDTLPALRYLTASLHVDY
                     LRPTPIDTLLEVRSSIKEIKGRKIVVSSMLFARGDLCAKGEVVAVQMPDHLIGGLPDS
                     "
     gene            3495..4646
                     /locus_tag="AC16CB_09355"
     CDS             3495..4646
                     /locus_tag="AC16CB_09355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3342715.1"
                     /translation="MDFALSEEQRMWRDAVHEFVSREVKPKARHVDETGEFNWDAVRK
                     MGPLGLLGLNIPEEFGGAGVDAVSAAIAIEELGWGCGSTALAIAAHNGLGCAPLALFG
                     SSELKRHFLSSVASGDGNLAALALTEPGAGSDLKGGVSVRASLDGNQWVINGAKMWCT
                     NASIADFIVTLVRTDPKGGSASLSMILVPTDSPGLHLGPPEKKMGLKGSPTHAVTYED
                     VRVPSDYLIGELGKGLQQTMVVLDGGRVSIGALSVGLAQAAFEEAIRYAQERQTFGQP
                     IANYQAIQWMLSNAATEIQAARLMVYYAAWLKEQGKPYSKEAAMAKMFASEMAERVCR
                     DAIQIHGGYGYSREYPVERIYRDARLMTIGEGTSEIQRLVIARHIMQEQ"
     gene            complement(4648..5247)
                     /gene="lepB"
                     /locus_tag="AC16CB_09360"
     CDS             complement(4648..5247)
                     /gene="lepB"
                     /locus_tag="AC16CB_09360"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017300325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR3342716.1"
                     /translation="MEYYRSEAYPDFDGQYDENGSRNFRRSLVDALETILLSIVLFLG
                     INAVSARIRIESVSMQPTLIAGDFVIVNKVAYKLGSPSRGDVIIFHYPPDPKREPYIK
                     RIIGLPGDVVQVGGGKVSVNDHLLNEPYISAPPQYQGEWKVPDDSLFVLGDNRNSSSD
                     SHQWGMVPVPNVIGKAELVYWPPSEWKLLNQDTAFAAEP"
     gene            complement(5387..5668)
                     /locus_tag="AC16CB_09365"
     CDS             complement(5387..5668)
                     /locus_tag="AC16CB_09365"
                     /inference="COORDINATES: protein motif:HMM:NF020967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative signal transducing protein"
                     /protein_id="HIR3342717.1"
                     /translation="MVSDTEWVAIETVPGDLQAEILRGLLEAQGIQVYLSQEGAGRAI
                     GLSVGPLGAVEILVPAESRQFALQVLEDYHAGLFINEAGLDEDDEMHSD"
     gene            5883..7466
                     /locus_tag="AC16CB_09370"
     CDS             5883..7466
                     /locus_tag="AC16CB_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342718.1"
                     /translation="MLMTQYSLREDYWDNFSLQQQDIEFIYNELLETETPLTSQELID
                     ILVKERIQREKLVIDQQRTADGDQYLPKERYAVSQKLVFPTLDWKKAKVVNVRPGNNP
                     ELGEFEVIQVAFEDGDVHEYAAGLQDHALNQPLNLAEVDATLDPDYVLNNYQEFLVET
                     LDEELIKNDDFVRIAGRWFPRALLLSINIGHLNLAEAVLDVAGGGPLPTTNLLEQIEI
                     PANVNPKLLEFSMDLALQEDERFDEVGAAGEVLWFLNRLEPEAVLEPPVYLRYPGIDY
                     DRSLLTIEMEALERELDDELSPLEGRPQDFEEVQLNLIYPHWRAGTLPLSSRLRYQFP
                     TAYEAPRIRFTLVDGLSGEKFPAWVVRPKRYVYGLREWYETHNLVPGSRIRIQRSQVP
                     GEVIIYPDTHRPSKEWVRTVLIGVDGGVVFAMLKQTIYSKFDERMTIAVPDVEAVDRL
                     WAQMSKERQPFEKVVVNTVRELAKLNPQGHVHASELYAALNVIRRCPPGPILTLLASR
                     PWFTHVGDLHFRFNDTERS"
     gene            7478..7912
                     /locus_tag="AC16CB_09375"
     CDS             7478..7912
                     /locus_tag="AC16CB_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342719.1"
                     /translation="MVEVKRVSLVKPALQTPFHIDFNWWAKNDRDWRVYLRSNLCIEH
                     QLAFEHVDLEEKVDWVDPVTAEVQQVDGLQNILITHCAKQPGFITEHSTLVDAAFRLF
                     LASGNAPMSAVELGERLGRPPETILRTFTGGRVYKGIRPCME"
     gene            7927..7999
                     /locus_tag="AC16CB_09380"
     tRNA            7927..7999
                     /locus_tag="AC16CB_09380"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7960..7962,aa:Arg,seq:tct)
     gene            8071..8592
                     /gene="tpx"
                     /locus_tag="AC16CB_09385"
     CDS             8071..8592
                     /gene="tpx"
                     /locus_tag="AC16CB_09385"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005835444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HIR3342720.1"
                     /translation="MSIVRKGLFEFRGQDAPVVGADIQVNQQAPEFTVQGMDWSLFRG
                     MGDTQGKVRIIGSLPSLSTSVCDRETRRFNQEAAALGEDIVIMMVSMDLPYTIKNWCG
                     AAGVDRVITVSDHLSGEFGEKYGVLLKDQRIFRRAIFVVDRNGKVVYAEYMPKMGDEP
                     NYEQVLTVAKDVL"
     gene            8661..9083
                     /locus_tag="AC16CB_09390"
     CDS             8661..9083
                     /locus_tag="AC16CB_09390"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3342721.1"
                     /translation="MINVVLKPDVQRPGFYILQDVRKLPEATRQRMAVRSHAWRPPTD
                     VFETEEMVIVRVEIAGMEETDFTIVLDGRYLHIRGVRSDVPERRAYHQMEIRFGDFSS
                     EVELPSDISLEHIEAVYSVGFLHIRLSKVRPVKIKVED"
     gene            9168..11747
                     /gene="lon"
                     /locus_tag="AC16CB_09395"
     CDS             9168..11747
                     /gene="lon"
                     /locus_tag="AC16CB_09395"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR3342722.1"
                     /translation="MPASRWTSNFLNPLNLDDQDDLTLEDLLLKQIVPQVIRPGEKDD
                     ESISEDEETGPVIPSVLPILPLRGLVVYPQTAVPLTIGQPRSIRLIDDVVAGENRLIG
                     LVASRDPELDTPEPKDLFNIGTAAMVHRLFRAPDGTIRLVIQGLARFHVGDYVQLEPY
                     LKANIELIPEKVDEGAELEALARSALNQFDRIAEMIPSIPRELVASILALEDPLQTVY
                     TIANFQRMELEDAQEILEIDSASDKLHKLVGILAHEVEVLEIGQKIQNEARSEIEKVQ
                     REYFLREQLKAIQKELGEGDEQAAEVEEFRRKIEAAKMTEEAEKQARRELDRLSRLPT
                     AAAEYGVIRTYLDWLVSLPWSVITEDILDINHASEVLDQDHYGLEDVKKRILEYLAVR
                     KLRLERSEELAAEPLDDIRRVREGVILCFIGPPGVGKTSLGRSIARAMGRKFIRISLG
                     GMRDEAEIRGHRRTYIGALPGRILQALRRIESRNPVFMLDEVDKLVFDFHGDPASALL
                     EVLDPEQNSEFRDHYLEIAFDLSQVMFITTANSLETIPGPLLDRMEVIRLSGYTESEK
                     IAIAQGYLIPRQLKENGLRTSEVVFLRPALEKIIHSYTREAGVRNLEREIGSVCRKVV
                     TRIAQGKSETEEVTPEKVQELLGRPKFYATEEVALRTSIPGVATGLAWTPVGGEVLFV
                     EATSMPGNKGFQVTGSIGNVMQESARAALSFVRSRANNLGLNPEFFEKSDIHLHVPAG
                     SQPKDGPSAGVTMATALVSLVCGCPVRADLGMTGEITLRGQVLPIGGVKEKVLAAHRI
                     GLKTVVLPVRNEADLEELPEEVRKDIQFIFAETVDDVLRAALESPPEKPKRRSKKKPE
                     EHQ"
     gene            11755..12120
                     /locus_tag="AC16CB_09400"
     CDS             11755..12120
                     /locus_tag="AC16CB_09400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3342723.1"
                     /translation="MPKVSLIEDDASMLSLLGTLLGFEGFEVAPLDGGMNLEETLENL
                     RRENPQLILLDVHLPAISSFDLLQRLRQDPELKSVQVLMSSGMELTDKCLQAGADDFI
                     LKPYMPDELIGKIRKMLKH"
     gene            12160..13911
                     /locus_tag="AC16CB_09405"
     CDS             12160..13911
                     /locus_tag="AC16CB_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HIR3342724.1"
                     /translation="MAKKKTSAVMQWRTMDLHLHTPASSDFLQPGVSYLEFLQRAESR
                     GLDIVSMTDHNTVSGYRRMKEEIHQLELLEKLNRLLPDEQKRLDEYRRLLKKILVLPG
                     FEFTATFGFHIMAVFSPDKPVREIEHLLLDLRIPPDQLDDGSATVGATSDVLTAYRLI
                     AEAGGLVIAAHANSSNGVAMRGFGFGGQTKIAYTQDPNLHALEVTDLEQKGKRNTAAF
                     FNGTKPEYPRRMHCIQGSDAHRLTADPQRKKNLGMGDRAVDVLIPEISFEALMELFTS
                     NDFARTRPHRHTEEPAFDFIHAAREEGANIVQDFHEGLAVRGGQRYAIIADVCAFANA
                     NGGTLYIGLSEDPNKNVVGVPDPEHAISQLEKEISNRISPPLHCSLDVHETEAKKIVR
                     ILVPGGDDPPYAVDDNKIYVRSEAETALAVRDEIVGLVQRGIPRQIPAVEAEEFQAVS
                     KAEVSLPEAPAQVEEEAPPRTGVEVVAVEEREGARYYTMRDLRNGNVVKNVTRTSARR
                     LWHYAITRFSRYPVDLSQAGIQWQGNLGLVHTQKQDKSVRYDLVQRTTQGYRYYFGVT
                     DDGIHGPWANLVGQEDD"
     gene            13904..14344
                     /locus_tag="AC16CB_09410"
     CDS             13904..14344
                     /locus_tag="AC16CB_09410"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3342725.1"
                     /translation="MIEGDELAFKIREFQYPQDYPAVSALWEASGPGVIPGRSDTPEE
                     TQKKLGRDPDLFLVAEVDDHIVGTVLGGFDGRRGLVYHLAVAKEYRGRGLGDSLMNEV
                     ESRLKEKGCIRAYLLIAHDNGGVVNFYKRRAWETMNLTVMAKDL"
     gene            14341..14868
                     /locus_tag="AC16CB_09415"
     CDS             14341..14868
                     /locus_tag="AC16CB_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HIR3342726.1"
                     /translation="MTLRIGILTVSDRSSRGERADTSGPAIQAVVKQQGWVVARTSVI
                     PDHLIAIQETLIDWVDSGEVDVILTTGGTGFSRRDVTPEATLAVIERAAPGISEAMRY
                     ASLQVTPHAMLSRAMAGIRGRTLVINLPGSPKGAVENLRVVLPVLPHAVQLLRDEPDA
                     EAGHLQILHSMEMVV"
BASE COUNT         3656 a         3555 c         4106 g         3626 t
ORIGIN      
        1 ccccgctggt acgggctata tataagctga gggcttgtga cattaagctg tgttagacag
       61 ggttttgtga cctatccaac tgatttactt tctctcagga cgacttgacg ttgctggcgc
      121 ctgggaagcg aaagacaaat ctggctggca aggcgcttac gatcaagtaa actatcagga
      181 aggttacaac cttatcaatt ggatcagaaa tagcgccctg ggcaaaggta gaagggagga
      241 tgccccaacc cattccccgg aaaatggcca ccaccacatc cgtgccagca ccggtgacac
      301 cgccgaatac gtaggctgag atcggcgcag acagagcaga tgcgaccaag ccagtaatca
      361 ggccggctag gaccactttc caccaagcgc gcaaccaacc cgattgagag aatagtccag
      421 ccagcgcgcc gatcacagcc gcaacggttg caaatggcgc gtaggtgaca tttagaccag
      481 tcaatcccca gattagattg gaaagcaagc cggtgagtgc tccggcccaa ggccctgcca
      541 gtacacccac gagtacggtg ccgatcgaat ctaggtagat ggggagcttc aaagtaatga
      601 tcagttggcc aatagcaata ttgatggcaa tggccacagg aattgtgacc agggtgaggg
      661 tgttaaaatc ctgcttgggg cgttccattg cgcacctcct tcaatgtggg taagtaatac
      721 tcgatggtct gcctggctat gattctacaa aatattgtac tacttatcct tggagtgtgg
      781 caggcttggt tttgtgtttt agaaacccct taggctttag atctagtgta aaccctaaac
      841 actcttttca cgggattgcg catgcccctc agggcatatg caattgccct gagggccata
      901 taaatttgtt tgagatcgat ttcaaagatg atacaattat taccagtatc tccaagtaca
      961 atcaaatttt ataaacggag cgtccatgag caatcaactc gagtttggag ggaatttcgt
     1021 ttgggaacct acgccagaat acgttgagcg tgctcatctg acccagttca tgggccagca
     1081 tcaaattaat gatttcaacg agttgatgca ccgctcaact caggatgtgg cctggttcac
     1141 tgatgcactt ctgcactatc tggatatacg cttctatcaa ccctatacac aggtgctgga
     1201 tcttagccgg ggcattgcct ggccaaaatg gtgtatagat ggccaaatga atattatcca
     1261 taattgcctg gataaatata ttggcacaga ggcggaagag cgtaccgcgc tcatctggga
     1321 gggcgaagaa ggggttgtca ggagattaac ctacaaggag ctttacagac aggtgaatcg
     1381 gacggcaaat gccctgcgca gcctgggatt gggtaagggc gatgctattg gcctttatat
     1441 gccaatgacc ccggaaatcg taatcgcgtt gctggcaata gctaagatcg gtggcgtaat
     1501 cttgccacta ttttctggct ttggcgccgg ggcagtcgct tcacgtttag cggacgctga
     1561 ggcgaaagcc ctcttcaccg ctgatgggtt tttccggcgt gccaagccgg tttcgatgaa
     1621 ggttgttgcc gatgaagctg ccaggatggt atcttcgttg cagcatctca tcgtggtcaa
     1681 tcgagcagaa atcgatgttc ccatgacagc cggtagagat cattggtggc atgagctggt
     1741 agattcgcag tctgatcagg cggatacgga agtcacgtcg gcagaagatg tgctcatggt
     1801 catctacacg tcaggcacca ccggccgacc caagggagct gtgcataccc attgcggatt
     1861 ccccgtcaag gccgcccaag atatggcatt tggcaccgat ctacatgccg gagaggtgct
     1921 gtattgggtc acggacatgg gctggatgat gggcccctgg ctggcatttg gcaccttgat
     1981 ccttggcggg acaatgttgt tgtatgacgg tgcgccagac tatccaggtc ccaatcgcct
     2041 gtgggcgttg gttgagcgcc atcgcatcac cgggcttggt gtttcgccca ctctggtgcg
     2101 ctcactcatc ccgcggggcg aggagcactt tctcaagcac gatctgtctt cgttgcgctt
     2161 gtttgcttcc acgggtgaag cctggaaccc cgatccgtgg aattggctct ttgagaaggt
     2221 tgggagaact cgtctgccag tgatcaatta ttcaggcggc acggagttat ctgggggaat
     2281 cgtgatgggg aacccgattt tgccgctcaa gccgtgtgcc ttttctgccc cctgcccagg
     2341 tattgccgct gatgtgctgg atgagaatgg aaatcctgtg cgcaaccagg ttggtgaact
     2401 ggtcatcaaa acgccatgga ttggcattac acggggtttc tggaaagacc cgcagcgtta
     2461 cgaggagacc tactggtctc gctggccggg gatctgggtt cacggtgatt tcgctttgat
     2521 agatgatgat gatatgtggt atatcctcgg tcgctcagat gacacgatta aggtagcagg
     2581 taagcgccta ggaccagccg aaatcgaatc aattctggtc agtcacccag atgtagtgga
     2641 ggctgcggca attggcgttc ctgacgagat caagggcagc gaggtggtgg ccttcgcggt
     2701 gctttcaaca gactgtgatc catgtgaaga gctgcgccaa gagttataca agatgatcgt
     2761 aaaagctctg ggcaagccct ttgctcctcg tgaagtcttg ttcgtcagcg acctgccaaa
     2821 gacgcgcaat gctaaagtga tgcggcgcat gatccgtgct gcctatttgg gggaggaccc
     2881 gggggacact tcatcgctgg tcaatccaga agctgtagaa gaaatccgcg tagcaaggta
     2941 aaacaaacac tcgatgagcg ataaagcctt tcaagatttc tactcagaac aatccagtca
     3001 ttgttacggt tgtggtcgat tgaacgagct cggcttacag atcaagagct attgggatgg
     3061 agacgaaacg gtctgcgttt tccagccgcg cccgtaccat accgccattc cgggttacgt
     3121 ctatggagga ctgatcgcct cgctgatcga ttgtcatagc acaggctcag ctgtcgcagc
     3181 agcttaccgt gcagcgggca gatctatgga tacactgcct gcattgcgct atctaaccgc
     3241 ctccctgcat gtcgattatt tacgcccaac tcccatagat acacttcttg aggtgcgttc
     3301 ttcgatcaaa gagatcaagg ggcgcaagat agtggttagc tcaatgctat ttgcgcgtgg
     3361 agatttatgc gccaaggggg aggtggtagc ggttcaaatg cctgatcatc ttattggtgg
     3421 cttgcctgat tcttaacggc attgatttgt gtcagaatca gaatgaacta taaacataat
     3481 tcggaggagc catcatggat tttgctttaa gcgaggaaca gaggatgtgg cgcgatgcgg
     3541 tccacgaatt tgtaagccgc gaggtgaagc ccaaagctcg ccatgtggat gaaacggggg
     3601 aattcaattg ggatgctgtc cgtaaaatgg gtcctctggg tttgttgggt ttaaatatcc
     3661 ccgaagagtt tggaggagcg ggtgtagacg cggtcagcgc agccatcgct atcgaagaac
     3721 tgggctgggg gtgcggcagc actgctttgg ctatcgccgc tcataacgga ttgggttgcg
     3781 ctccgcttgc cctatttgga tctagtgagc ttaaacggca ctttttatcg tctgtagcca
     3841 gtggagatgg caatttggct gccctggcgc ttaccgaacc tggagccggc tctgacctga
     3901 aaggtggggt gagcgtgcgt gcttctctgg acggcaacca atgggtgatc aatggagcaa
     3961 agatgtggtg caccaatgcc agcatcgcgg atttcattgt taccctagtg cgcaccgatc
     4021 caaaaggcgg ttcggcttcc ctgagtatga tccttgtccc cacagattcc cctgggcttc
     4081 accttggtcc cccggaaaag aaaatgggat taaaagggtc cccaactcat gctgtcacct
     4141 atgaggatgt gcgtgtgcca tccgattacc tgattggtga attagggaaa gggttgcagc
     4201 agaccatggt tgttctggat ggagggcggg tcagcatcgg agccttatcg gttggtttgg
     4261 ctcaagccgc atttgaggag gcaattcgct atgcccaaga gcgccaaacc tttgggcaac
     4321 ccatcgcaaa ctaccaggcg atccagtgga tgctttcgaa cgctgcaacc gagattcaag
     4381 ctgctcgcct gatggtgtat tacgcggcat ggctaaaaga acagggaaag ccttacagca
     4441 aggaagccgc tatggcaaag atgtttgcca gcgagatggc tgagcgcgtc tgccgtgatg
     4501 ccatccaaat tcatgggggt tatggctaca gccgtgaata tcctgtggaa aggatttacc
     4561 gggatgctcg attgatgact attggggagg gaaccagcga gatccaacgc ctggtaattg
     4621 ccagacacat catgcaagag caataaacta aggctcagcc gcgaaggctg tatcttggtt
     4681 gagtaatttc cactcgcttg gcggccagta caccagctca gcttttccga tcacgtttgg
     4741 tacgggcacc atgccccact ggtgggagtc ggatgagctg tttctgttat cacctagcac
     4801 aaagagagag tcatcgggca ccttccactc tccttgatat tgaggtggtg cagaaatgta
     4861 cggttcgttc agcaagtggt cattgacgga taccttgccg ccacccacct gtacgacatc
     4921 acccggcaaa ccgatgattc gcttgatata aggttctctc tttggatcgg gggggtaatg
     4981 aaagataatt acatcaccgc gactcggtga gcccaatttg taggcaacct tattgacgat
     5041 cacaaaatcg cctgcaatta atgtcggttg catgctgacc gattcaatac gtatgcgggc
     5101 tgatactgcg ttgataccta agaataagac aatggacaaa agaatggtct cgagagcgtc
     5161 caccagactt cggcggaaat tcctcgatcc attttcatcg tactgtccat caaaatctgg
     5221 atatgcctca gatcgataat attccatcaa atcgcctcaa atcaagatcg taattacgca
     5281 ccaggaagct ggcggggctc agccttcaaa tgcgtaaata ctacaagcat tataccccct
     5341 gagctgaggc aatggataca ggacaaaggc actatttaat gagatattaa tcgctgtgca
     5401 tttcatcgtc ttcgtccagg cccgcctcgt ttatgaatag gccggcgtgg taatcttcta
     5461 aaacctgcag cgcaaactgg cggctctcag ctggaacgag aatttcgacc gcacccagag
     5521 ggccgacgct taacccgatg gcgcggccgg caccttcctg ggaaagatat acttggatcc
     5581 cctgcgcttc caacaagcct cgcagaatct cggcttgtaa atcaccggga actgtttcaa
     5641 tagctaccca ctcagtatcg gataccatat ctatcctcca gttcttaagg caacagtaga
     5701 gctcatctca aaaatgcttg attgggatga tttgcagcag cgaatccgtt gcgaaacccc
     5761 ctgccacgta tccaactgcg taacttctat gagttttatt attgctcgaa ataggttaat
     5821 ccatcctttt tccatcgggt ataatattaa cgcataatat taggcagatc aaaggcaatt
     5881 ttatgttaat gacacagtat tcgcttcgtg aagattactg ggacaatttt agtctccagc
     5941 aacaagatat cgaatttatt tataatgaat tattggaaac ggaaacgccc ctaacctctc
     6001 aggagctgat agacatcctg gtgaaagaac gcatccaaag ggaaaagctt gttattgatc
     6061 aacagcgcac cgcggatggc gatcaatact tacctaaaga acgctacgct gtatcacaaa
     6121 aattggtgtt tccaacatta gactggaaaa aagcaaaggt agtgaatgta cgcccgggaa
     6181 ataatcctga actgggagaa ttcgaagtta tacaggtggc ttttgaggat ggggatgtgc
     6241 acgaatatgc tgccgggttg caggatcacg cgctgaacca gccgcttaac ctggctgagg
     6301 ttgatgcgac cttagacccg gattacgtgc tgaataatta ccaggagttt ttagttgaaa
     6361 ctttggatga agagctgatc aaaaacgatg attttgtgcg cattgctggt cgctggttcc
     6421 cacgggcatt gctattgagc atcaatatcg gtcatctaaa tttggcggag gcagtcctgg
     6481 atgtggcagg gggaggtccc ttgcccacca ccaacctatt ggagcaaatc gagattcctg
     6541 ccaacgtcaa cccgaagctg cttgagttct ctatggatct cgcgcttcag gaagatgagc
     6601 gcttcgatga agttggcgca gccggtgagg tgctttggtt cctaaaccgc cttgagccag
     6661 aggctgtgct ggagccgcca gtttacttgc gttatccagg gattgattat gatcgctcgt
     6721 tgttaaccat cgaaatggaa gcgctcgagc gcgaactgga tgatgagctt tcaccattag
     6781 aggggaggcc ccaagatttt gaagaagttc aattgaatct gatctatcct cattggcggg
     6841 caggcactct gccactttca tcgagattgc gctatcaatt tcctacagct tatgaggcgc
     6901 cgcgcatccg ctttaccttg gtagatggcc ttagtggtga aaaatttcca gcctgggtcg
     6961 tgcgccctaa acggtatgtt tatgggttga gagaatggta cgaaactcac aatttagtac
     7021 caggaagcag aatacgcatc cagcgtagcc aagtgccagg ggaagtgata atttacccag
     7081 acacacaccg tccgagcaag gagtgggtgc gcaccgtgtt gatcggtgtg gatggcgggg
     7141 tggtttttgc catgctcaag caaacgattt attcgaaatt tgatgagcgc atgaccatcg
     7201 ccgtgccaga tgtggaggca gtcgatcgcc tttgggctca gatgagcaag gagcgccaac
     7261 ctttcgaaaa ggtggttgtt aacaccgtgc gcgagctggc gaagttaaat ccacagggtc
     7321 atgtgcatgc cagcgagcta tatgctgccc tgaatgtgat taggcgctgt ccacctggac
     7381 ccatcttgac attgcttgca tcccgtcctt ggtttacaca tgtaggcgat ttacattttc
     7441 gcttcaacga taccgaacgg agttagtgga gggaaatttg gtcgaagtca aacgtgtcag
     7501 tctcgtcaaa ccagcgctgc aaaccccctt tcacattgat ttcaattggt gggcgaaaaa
     7561 tgaccgggat tggcgtgttt atttgcgtag taatttgtgc atagaacacc aattagcatt
     7621 tgaacacgta gatttagagg aaaaagttga ttgggtcgat ccagtaactg ctgaagtaca
     7681 gcaagtagac ggcttgcaga atatattgat cacccattgt gccaaacaac caggattcat
     7741 aacagaacac tcaaccttgg tcgatgcagc attccggctc ttcctcgcca gcggcaatgc
     7801 cccgatgagt gccgtggagt tgggagagcg cctcggtcgc cctccggaga cgatcttgag
     7861 aacctttacc ggcggtcgtg tctacaaggg cattcgtccg tgcatggaat aatcgacagc
     7921 atgcttgccc ccgtagctca aaggatagag cacctgcctt ctaagcagtt ggctgtgggt
     7981 tcgaatcccg ccgggggcgc ctgattgtca ttgatagcaa tttgtttgtt atctcttttg
     8041 ttcaacagcg aattctaagg gagaaaatta atgagtattg ttcggaaagg tttgttcgaa
     8101 tttcgtggcc aggatgcccc agtggttggt gcagatattc aggtaaacca gcaggcgcct
     8161 gagtttactg ttcaggggat ggattggtct ctttttaggg gaatgggtga tacccaaggc
     8221 aaggtgcgca ttatcggttc gctcccctcg ctgagtacgt cggtttgtga tcgcgagacg
     8281 cgccgtttca accaggaagc tgctgcgctg ggggaggata ttgtcattat gatggtgagt
     8341 atggatctgc cttatacgat caaaaattgg tgtggtgcag caggggttga cagggtgatc
     8401 accgtgtcgg accatcttag tggagagttt ggcgaaaagt atggcgtatt attaaaagat
     8461 caacggatat ttcgcagggc aatcttcgtc gtcgatcgta acgggaaggt tgtttatgcg
     8521 gaatatatgc cgaaaatggg tgatgaacca aactatgagc aggtattaac ggtagccaag
     8581 gacgttcttt aagcatgggg tttcaggtat tcgattgaag gctggttgaa tgatcttcaa
     8641 ttggaaacca aggtggtcat atgataaatg ttgtcttaaa acctgatgtg caaagacctg
     8701 gtttttacat tttgcaagat gttcgaaaac tcccagaggc aacccgccag cgtatggcag
     8761 tgcgttcgca tgcatggcgc ccacccacgg atgtgtttga aaccgaagag atggtaattg
     8821 tccgcgtgga aatcgccggt atggaggaga ccgacttcac gattgtctta gatgggcgtt
     8881 acctccatat tcgcggagta cgcagcgatg ttcccgaacg gcgcgcttac caccagatgg
     8941 aaattcgctt cggtgatttt agcagcgaag tggagcttcc atccgatatt tctttggagc
     9001 acatcgaggc ggtttacagt gtaggttttc tgcatattcg tttatctaag gttcgcccgg
     9061 taaagattaa ggtcgaagat tagagtgaat ctacccggat gactgcttca tcagaagaga
     9121 aattgatcat gaggcgctcg tcggatccag aaaataaaag agataaaatg cccgcatctc
     9181 gttggacgtc caacttttta aaccctctca atctggatga tcaggatgac ctaactttgg
     9241 aagatttgct attgaagcag attgtccccc aggttatccg gcctggagag aaggatgatg
     9301 aatccatatc agaggacgaa gagactggac ctgtaatccc gtctgttctt ccaattctgc
     9361 ctttgcgcgg tctggtggta taccctcaga ctgcggtgcc gctaacgatt ggacagccac
     9421 gttccattcg actgatagac gatgtcgttg ctggagagaa tcggcttatt gggctggtag
     9481 cctcccgaga tccggaactt gataccccag aacccaagga cttattcaat attgggaccg
     9541 cagcgatggt tcaccgctta tttcgggcgc cggatggcac catccgtctg gtcattcagg
     9601 gtttggcgcg ctttcacgtg ggagattatg tccaactaga accctacttg aaagccaata
     9661 ttgagctaat tccagaaaag gtagatgagg gagcagagtt agaggctctg gcgcgtagcg
     9721 ctcttaacca gtttgatcgc attgcagaga tgatcccctc aatccctagg gagttggttg
     9781 catccattct ggctttggaa gacccgttac agacggtgta taccattgcg aattttcagc
     9841 gcatggagct agaagatgcg caagaaatcc tagaaataga ttcggcttcc gataaactgc
     9901 ataagctggt tggtatctta gcccatgagg tcgaggttct tgagatcggg caaaaaatcc
     9961 agaatgaagc ccgttcagaa attgaaaaag tgcagcgaga atatttcctg cgcgagcagc
    10021 ttaaggccat tcagaaagag cttggtgaag gcgatgagca ggctgcagag gttgaagaat
    10081 ttcgcaggaa gatcgaagcc gccaagatga ctgaggaggc cgaaaagcaa gcccgtcggg
    10141 agctagaccg tctttcacgc ttgccaactg ctgcagccga atatggggtc attcgaacgt
    10201 atcttgattg gctggtttct ctgccttggt cggtgatcac agaggatatt ctggatatca
    10261 atcatgcgag cgaggtctta gaccaggacc attatggctt ggaggatgtc aaaaaacgca
    10321 ttttggagta cttggcggta cgcaagctgc gattggagcg ctccgaagaa ctggcagctg
    10381 agccacttga tgatatccgc cgcgtgcgtg agggcgtcat tctgtgcttc ataggtcctc
    10441 caggggtggg caaaacctcc ctcggtcgat ccattgcacg agctatgggg cgaaaattta
    10501 tccgcatttc cttgggaggc atgcgtgatg aagccgagat ccgcggccac cgccggactt
    10561 atatcggcgc tctgcctggg cgaatcctac aagcactgcg tcgtatcgag tcccgaaacc
    10621 cggtatttat gctggatgaa gtcgataagc tagttttcga tttccacggc gacccggctt
    10681 cggctttatt ggaggtgctc gacccagagc aaaactcgga atttcgcgac cattacctgg
    10741 agatcgcctt cgatctttct caggtaatgt tcatcaccac tgccaattcc cttgaaacca
    10801 tacctgggcc gcttcttgac cgcatggagg tcatccgcct gtccggttat accgagagtg
    10861 agaagatagc cattgctcag ggatacctga tcccccgcca attaaaagaa aacggtctgc
    10921 gcacatcgga agtggttttc ctccgccccg cgttggaaaa aatcatccac tcctacaccc
    10981 gcgaagctgg cgtgcggaat cttgagcgag agatcggatc tgtctgccgc aaagtagtta
    11041 cccgcatcgc tcagggcaag agcgaaacgg aggaggtgac tcctgaaaag gtacaggagc
    11101 tacttggacg cccgaagttt tatgccactg aggaggtagc cttacgcacc tcgataccag
    11161 gcgtggcgac tggtctggct tggacacctg taggaggcga ggtgctcttt gtcgaggcca
    11221 ccagcatgcc cggcaataaa ggttttcagg taaccggctc gatcggcaac gtgatgcagg
    11281 agtcagctcg ggctgctctt tcgtttgtac gctcgcgcgc caacaacctt ggcttaaacc
    11341 ctgaattttt cgagaaaagc gacattcatc tccatgttcc ggccggctcg cagccaaagg
    11401 atggaccatc ggctggtgtg accatggcaa ctgctctggt ttcgctggtg tgtggttgcc
    11461 ccgtgcgcgc agatctgggc atgacgggtg agatcacctt gcggggtcag gtattgccta
    11521 taggtggtgt gaaggagaaa gtattggcgg cgcaccgcat cggattgaaa actgtggtgc
    11581 taccagtccg caatgaggcc gacctggagg agctgccaga agaggtgcgt aaagatatcc
    11641 aatttatctt tgctgaaacg gtagatgatg tgctgcgcgc tgccctggaa tcccctcctg
    11701 agaaacctaa acggagaagc aaaaaaaagc ctgaggagca tcaataagat catcatgccc
    11761 aaagtatcct taattgaaga cgacgcctcc atgttatccc ttttaggtac tttgttggga
    11821 tttgagggat ttgaagttgc cccattggat ggtggaatga atctcgaaga gactcttgag
    11881 aacttgcgca gagagaatcc ccagcttatc ttacttgacg ttcatctgcc tgcaattagc
    11941 agttttgacc tgttgcaacg gctgcgccaa gatcctgaat tgaagtctgt gcaggtgctg
    12001 atgtcttcgg gtatggaact gactgataaa tgccttcaag ctggggcaga cgattttatc
    12061 cttaaaccat atatgcccga tgaactcatc ggaaagatcc gtaaaatgct caaacattaa
    12121 cccaaaaatg ggtaaatcaa gaaagtacac cgataaaata tggcaaagaa aaaaacaagc
    12181 gccgtgatgc aatggcgcac catggatttg catttacaca ctccagcttc tagcgatttc
    12241 ttacagcctg gggttagcta cctagagttc ttgcagcgcg ctgagtcccg cggtctggat
    12301 attgtttcta tgaccgacca taatactgtt tccggctatc ggcgcatgaa agaagagatt
    12361 caccaactcg agcttttaga gaaactaaat cgactcttgc cagacgagca aaagcgattg
    12421 gacgaatacc gccgtcttct taaaaaaata ctggtattgc ctggctttga gttcactgcc
    12481 acttttggct ttcacatcat ggctgttttc tcaccagata agccggtgcg tgagatcgag
    12541 catcttttgc tagatctgcg aatcccgccc gaccagttag atgatggatc ggctactgtt
    12601 ggtgcgacaa gcgatgtgct gacggcttac cgactgatcg ctgaagctgg tggtctggtg
    12661 attgctgcac atgccaattc cagcaacggc gtcgctatgc gcggatttgg ttttggcggg
    12721 cagaccaaaa tcgcctacac tcaagatccc aatttgcatg ccctggaagt gacggatctg
    12781 gagcaaaagg gtaaacgcaa cactgcagcc ttctttaacg gcaccaaacc ggagtaccca
    12841 aggcgtatgc actgcatcca gggctctgat gctcatcgtc tgacagccga cccccagcgg
    12901 aagaaaaacc tgggtatggg tgatcgagcg gttgacgtac tcatcccaga gattagtttc
    12961 gaagccttga tggagttatt caccagcaat gactttgccc gcacccgtcc gcatcgccac
    13021 acagaagagc cagcatttga ttttatccac gctgcccggg aagaaggcgc caacatcgtc
    13081 caggattttc atgagggctt agcggtgcgc ggtggacagc gttatgcaat catcgccgat
    13141 gtatgcgcct ttgccaatgc caatggcggt acgctgtata tcggcctaag cgaggatccg
    13201 aataaaaacg tggtgggagt gcccgatcca gagcacgcca tctcccaatt ggagaaggag
    13261 attagcaacc gcatcagccc acccctgcat tgctcgctcg atgtccacga aaccgaagcg
    13321 aagaagatcg tccgcatcct cgttcccggt ggtgacgatc ctccatatgc tgtcgatgac
    13381 aataagatct atgtgcgctc tgaagccgag actgccctgg cggtgcggga tgaaattgtg
    13441 ggtttggttc agcgagggat tccgaggcaa atacccgcag tagaggcgga ggaattccaa
    13501 gctgtatcga aagctgaagt atcattacct gaagcacctg ctcaggtaga agaagaggcg
    13561 cctccgcgca cgggggttga agttgtggcc gtggaggagc gtgagggggc gcgctattac
    13621 acaatgcgcg acctgcgcaa tggaaatgtg gtgaaaaacg tcacgcgtac ttcagcgcgc
    13681 cgtctgtggc actatgccat cacccgattc tcacgctatc ctgttgatct atcccaagct
    13741 ggtattcagt ggcaaggaaa tctgggcctg gtgcatactc agaagcagga taagagtgtt
    13801 cgctatgacc ttgtgcagcg cactactcaa ggttatcggt actactttgg tgtcaccgat
    13861 gacggtatcc acggtccttg ggctaacctg gtaggacagg aagatgattg agggtgatga
    13921 actggctttc aaaatccgtg aatttcaata cccccaggat tacccagcgg tcagtgcttt
    13981 atgggaggcc tcaggacctg gggtgattcc tgggcgatcc gataccccgg aagagacgca
    14041 gaaaaaacta ggacgcgatc ctgacctttt cctggtggct gaagtggatg atcatattgt
    14101 cggcaccgtc ttgggaggct ttgatggacg gcgcggcctc gtgtaccatc tcgcggtagc
    14161 caaagagtac cgtggtagag gcttggggga ttctttgatg aatgaagtag aaagcaggct
    14221 gaaagaaaaa ggctgcatcc gggcatattt gctgattgct cacgataacg ggggtgttgt
    14281 gaatttttac aagcgccgtg cctgggagac gatgaatctc acggtcatgg cgaaggattt
    14341 atgaccttac gcatcggcat cttgaccgtt tctgatcgct cctcccgagg agagcgcgct
    14401 gatacttctg gaccagcaat acaggcagtc gttaagcagc aggggtgggt ggtagccagg
    14461 actagcgtca ttcccgacca cttgatagct atccaggaga cgctgatcga ttgggtcgat
    14521 tcgggggaag tggatgtaat tttgaccacc ggggggacag gctttagtcg gcgcgatgtg
    14581 accccagaag cgacgcttgc ggtcattgag cgagcggcgc cgggaatctc tgaagccatg
    14641 cgctacgcaa gcttacaggt cacgccgcat gccatgctat ctcgggccat ggcaggcatc
    14701 cgagggcgaa cgctggtgat caatttgcct ggaagcccta agggtgcagt ggagaatttg
    14761 cgggtcgtac tgcctgttct gccacatgcc gtgcagcttc tgcgcgatga accggatgca
    14821 gaggcaggac acctgcaaat tctccactct atggaaatgg ttgtataagc cagtcaaggg
    14881 gatcgacctg aatcccgttc acccaaactt cccagtgaag atgggggcct tctacgcgac
    14941 ccg
//
LOCUS       DBNGSX010000311         5588 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1688333, whole genome shotgun sequence.
ACCESSION   DBNGSX010000311 DBNGSX010000000
VERSION     DBNGSX010000311.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5588)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1688333"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="AC16CB_09420"
     CDS             complement(<1..631)
                     /locus_tag="AC16CB_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342727.1"
                     /translation="MKHGYWSLASIFVLVIITSLTRAWVAKANQSEAQEPDPTPVSHS
                     ITPSNPDPFTSTPQFTATDIFTPTSTLEPTSTIIPATPTVTPITSPTQLTEQFNAESP
                     TESSSPASIYLPLILRQDAPLPPPNYTPPVKILICDSLSSPKSIPDNYDLGINDTINI
                     SDQRKIVDIDLRLEISHTGVGDLNVELTHLEGNQSVKLINRPGRSGSGLG"
     gene            762..1457
                     /locus_tag="AC16CB_09425"
     CDS             762..1457
                     /locus_tag="AC16CB_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342728.1"
                     /translation="MYKLSITTVILVFSLGLTACSQLNPTDTGELIFPTVGPDKPLDT
                     QFPLPGDLPVALQTPTIEALAPVQPTQTVESPVIPQINVERSVSSLETPVYRVQAGSP
                     VAMANFIQPEAGCNFMGVGGQPFDLSGQPVVQLVVEVGGSLAGSDVFHLQLSGNAPVL
                     GPGGFHVVLSDHPIESNGTLWILLYDLAGQPLTEKIYFSTYNDCSRNFTLINFVQVDP
                     SSDYRINFPIIYR"
     gene            1576..1815
                     /locus_tag="AC16CB_09430"
     CDS             1576..1815
                     /locus_tag="AC16CB_09430"
                     /inference="COORDINATES: protein motif:HMM:NF015956.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR3342729.1"
                     /translation="MQLIDAGNDEWLRVVNIIGGKGVAHKLRQLGVMPGDCIRIIRHA
                     PLGGPIMIEVGGRVIAIGRGVASHIDVEKSECASL"
     gene            1800..3710
                     /gene="feoB"
                     /locus_tag="AC16CB_09435"
     CDS             1800..3710
                     /gene="feoB"
                     /locus_tag="AC16CB_09435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HIR3342730.1"
                     /translation="MRFALIGQPNCGKSTLFNQVAGYKAETGNFAGTTVNYTESKVRV
                     AGEVIELVDLPGTYSLTGTNPAERETLRYLGSRQVDVIINVIDSTHLSQGVSLSIELL
                     ELQHPMVIALNMMDEAAHMGLKIDGAQLEELFGIPVLPLVASKGRGVRKVFLSALAIA
                     KSRKTPKRPSYSLDIEEAIQSLATFLDGKDAYWDAEPLAIKLLEGDEALIAQVATQTP
                     GLISQIQSAQQRLEQIRGQSSVWTISGERHGLAANLAGKVVEQGERKIILRDRLDNLL
                     LHPIWGYFFLGAILFIFFEVIYGLGSALEAPLIGFFNNLDLTLSTLLGSGTLGSEILV
                     GILQGISGGVAIVLPYLLPFLIGLGFLEDVGYLPRVAFLMDALMHRIGLHGKAVVPFI
                     LGYGCNVPAVMSTRMLEDRRDRFLAAALATLVPCAARLAVVFGLVAFYLGPRLALGIY
                     VFNLFVIAVTGRLLTSLMPEETPGLILEIPVYRMPTLRNIFFKVWFRAREFVVEAWSI
                     LILGSVVLAILNYFQFADIFNVLVRPLTWVMGLPERVGVPLIFGILRKELSLIMLRQA
                     LGVTDFADGLTPIQMVTFTVFVVFYIPCLATLAVLKRELGWREMFLIALLTVVIALLA
                     ALVARGIGLLVL"
     gene            3743..4315
                     /locus_tag="AC16CB_09440"
     CDS             3743..4315
                     /locus_tag="AC16CB_09440"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3342731.1"
                     /translation="MKIKTLDHFGFIAPFYDKAIPLRRAEKLIELARLPVDGRLLDAG
                     GGTGRVAQSLKRYVSRVVVADLSIGMLSEAKAKDGLSLICSFSESLPIPDETFERIIM
                     IDALHHVLDQQETAAELYRVLKPGGRIVIEEPDIGKFMVKLIAIAEKILLMRSHFLSP
                     PKIVELFRPFKTNTQIVREEFSAWVIIDKT"
     gene            4355..4948
                     /locus_tag="AC16CB_09445"
     CDS             4355..4948
                     /locus_tag="AC16CB_09445"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR3342732.1"
                     /translation="MHIVLDIALAAFGYGLGSLPFAIWVTRWLKGVDVRASGSRHATA
                     TNTFRQVGFAGGVFVFILDLTKGFFAVYLARTYGNSEWVAPLSAALVVIGHCWPVTAG
                     FRGGMGLATSGGAILAVNFIWFMIGVGLLVVLVLLIRHSARASVFASLSLPWGVWIAG
                     FRGELFWTTLCIGIVVAIRFLDDWNREYRELWLDRPD"
     gene            complement(4951..>5588)
                     /gene="hypE"
                     /locus_tag="AC16CB_09450"
     CDS             complement(4951..>5588)
                     /gene="hypE"
                     /locus_tag="AC16CB_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HIR3342733.1"
                     /translation="AGDTKVVEKGKADGLYINTSGIGLVMPGVEIGGEHARPGDKVIL
                     SGPIGDHGIAVLAARGELGFEAEIMSDVAPLNKLVASILEVSDRVHVLRDPTRGGLAT
                     SLNEIARQSKVGIMLDESSIPVRPAVQAACEMLGFDPLYVANEGKLIAIIGKEDVDNV
                     IVAMRKQAYGTQTCIIGEVIEAPQERVLMKTSIGSTRIVDTLSGELLPRIC"
BASE COUNT         1281 a         1188 c         1497 g         1622 t
ORIGIN      
        1 aacctagacc tgatccagaa cgaccggggc gattaattaa ttttacggat tgattgcctt
       61 ctaaatgagt tagttccaca tttaaatcac ccaccccggt atgggatatc tccagccgga
      121 gatcgatatc gacaatcttg cgttgatcgc tgatgttgat cgtgtcgttg atgcccaggt
      181 cgtaattgtc tggaatcgat ttgggcgaag agaggctatc gcaaattaga attttgaccg
      241 gtggtgtgta gtttggcggt ggaagagggg catcttgccg gaggataagc ggaagataaa
      301 tcgatgcagg ggacgagctt tcagttggcg attcggcgtt aaattgttcg gtaagttgcg
      361 tcgggctggt tattggggtt acggtgggtg tggcggggat aatagtgctt gtcggctcca
      421 gggtcgaagt gggagtaaaa atatctgtag ctgtgaattg tggtgttgag gtaaaaggat
      481 ctgggttgct gggtgtgatc gaatgggaaa caggtgtggg gtcaggctcc tgcgcctccg
      541 attgattggc tttcgccacc caagcgcgcg tcaggctggt tataataacc agtacaaaga
      601 tagatgcaag cgaccaatat ccatgtttca tgcgggtttg aatgtatttc tgctacattg
      661 tgcttgcgct cgtaattgga accaatctta ccataaccat gaggtacttc gatattgggc
      721 atgaaacttg taacacctgt cagtggatga aggagcatgt gatgtataag ctgtcgatta
      781 ctactgtcat tcttgttttc tcccttggct tgactgcatg ttctcaactt aatccaactg
      841 ataccggaga actaatcttc ccaaccgtag gacctgataa gcccttggat acgcagttcc
      901 cattgccagg tgatttgccg gtcgcgttgc aaacccctac gattgaagcg cttgctccag
      961 tccagccaac gcaaacagtg gagtcgcctg taattcccca gataaacgtg gagaggagtg
     1021 tgagttcact cgagacgccc gtttatagag ttcaagctgg ctcacccgtt gccatggcta
     1081 actttatcca gcctgaggcg gggtgtaatt ttatgggtgt tggtgggcaa ccatttgatt
     1141 tgagcgggca accggtcgta caacttgttg ttgaggttgg cggttctcta gctggctctg
     1201 atgtttttca tctgcaatta agcggcaatg cccctgtttt gggccctgga ggttttcatg
     1261 ttgttctgtc ggatcatccc attgaatcga atgggacgtt atggatttta ctttacgacc
     1321 ttgcaggaca acccctgaca gagaagattt atttttccac ttataatgat tgctcacgca
     1381 attttacttt aatcaacttt gtccaagttg atccttcttc tgattaccgc atcaatttcc
     1441 caataatata tcgataaaca tatactgact tgctgtccta gtattgtctc taaaagtacc
     1501 caacccaagt atattaatct tacgggtagt tgggatataa tgcctgtgcg attttacttg
     1561 gtttacgagg cttgaatgca gttaattgat gctggcaacg atgaatggct acgagtggtt
     1621 aatattattg gtggtaaagg ggtggcacat aaattacgcc aattgggcgt aatgcccggg
     1681 gattgtatcc gaattatccg ccatgcccct ttaggtggac ctataatgat cgaggtgggt
     1741 ggacgagtca tcgcaatcgg cagaggggtg gcgtctcaca tcgatgtgga gaaaagtgaa
     1801 tgcgcttcgc tctaattggg cagccgaatt gcggaaaaag tacgctcttt aatcaggtag
     1861 cgggctataa ggccgaaaca ggtaattttg caggtacaac cgtcaattat acagaaagca
     1921 aagtgcgtgt agccggagag gtaattgagc tagttgactt gcctggcaca tacagcctga
     1981 cggggactaa tccagctgag cgagaaacct tgcgctatct gggttcacga caggtcgatg
     2041 tgatcatcaa cgttattgac tccacccatc tctcgcaggg ggtaagctta tccatagaat
     2101 tgctggagct tcagcatccg atggtgatag cactcaatat gatggacgaa gctgcgcata
     2161 tgggcctaaa gattgatggt gcgcagcttg aggagttgtt tggaattcca gttctgccat
     2221 tggtagctag caagggccgc ggggtgagaa aggtgtttct ctccgccctt gcaattgcaa
     2281 aatcaagaaa gacgccaaag cgacctagct acagtcttga tatcgaagaa gcaatacaat
     2341 ccctggcgac atttttggat ggcaaagacg catattggga tgctgaaccc ctggcgataa
     2401 agcttctaga gggagatgaa gccctgattg cgcaggttgc cacccagacc cccggtctaa
     2461 tctcccaaat tcaatctgcc caacaaagat tggagcaaat tcgaggccag tcatctgtct
     2521 ggacgatcag cggtgagcga cacgggcttg cggctaacct ggcgggaaag gtggtcgagc
     2581 aaggtgaacg caagattatc cttcgtgaca ggctggataa tttgttgctc catccaatat
     2641 ggggttactt cttccttggt gcgatacttt ttatattttt cgaagtgatc tatggactgg
     2701 gaagtgcgct ggaagcccct cttattgggt ttttcaataa tttagacctg acattgtcta
     2761 ccttattagg atcgggtaca ttaggcagtg agatacttgt aggtatcttg caaggaatct
     2821 ctgggggagt agctattgtc ttgccgtatc tactcccatt cctgattggg ctgggatttt
     2881 tggaggatgt aggttatctg cctcgggtag cttttttgat ggatgcttta atgcatcgaa
     2941 ttggattgca tgggaaagcc gttgtgcctt tcatcctggg ttatggatgt aatgtgccag
     3001 cagtgatgtc tacgcgtatg ctggaagatc gtcgcgatcg gttcctggca gccgcattgg
     3061 caactctggt gccatgcgcg gcccgcctgg ctgtcgtttt tgggcttgtg gcattttacc
     3121 ttggcccgcg attggcgttg ggcatctatg ttttcaacct gtttgttatt gcggttacag
     3181 gacgattgct taccagtttg atgccagaag agacaccggg attgatactt gaaatacctg
     3241 tatatcgaat gccaacgttg cgtaacattt tctttaaagt ttggttcagg gcgcgggaat
     3301 tcgttgttga agcctggtcg attctcatac ttggaagtgt ggtactggct atattaaatt
     3361 actttcaatt tgcggacatc ttcaatgtcc ttgtccgtcc gctcacttgg gtaatggggt
     3421 tgcccgaacg agttggcgtt ccgctcattt ttggtatttt gaggaaggag ctatctttga
     3481 ttatgctgcg gcaggcattg ggtgttactg actttgcgga tggacttaca cctatccaga
     3541 tggtgacatt taccgtgttt gttgtattct acatcccctg cttggctacc cttgcggtgc
     3601 tcaagcgcga gcttggctgg cgggagatgt tcctgattgc ccttctaact gtggtaatcg
     3661 cgctgctggc agctcttgtg gctcgaggga ttggattact ggttttataa gtttgacgat
     3721 atagcggaat gatgatgtgc tcatgaaaat caaaacattg gatcactttg gatttattgc
     3781 accattttat gataaggcta tccctttacg ccgggcagag aagttgatcg aactggctag
     3841 actgccagtg gatgggagat tgcttgacgc aggtggcggt acgggacggg tagctcaatc
     3901 gctcaagagg tatgtttcga gggtcgtggt tgctgattta tcaattggca tgctctctga
     3961 agccaaagcc aaagatggtc tatcgctgat ctgttccttc tctgaatctt tgcctatccc
     4021 ggacgaaaca tttgagcgta ttattatgat cgatgcattg catcatgttt tagaccagca
     4081 ggagacagct gctgagctat accgggtctt gaagccagga ggccgaattg taattgagga
     4141 gccagatatc gggaaattta tggtcaaatt gatcgcgatt gcggagaaaa tactcctgat
     4201 gcgaagccat tttctctccc cgccaaaaat cgtcgaatta tttcgtccat tcaaaacaaa
     4261 cacacagatt gtgagggaag agttctccgc ctgggtgatt attgataaga cttgaaataa
     4321 accaaaaaat gcttaagtaa atcggggata tctgatgcat atagttctcg atatagcatt
     4381 agctgcattt ggttatgggc ttggatcgct gccattcgca atttgggtca cgcgctggct
     4441 caaaggtgtg gatgtgcgcg caagtggatc tcggcatgct acggcgacaa atacattccg
     4501 tcaagtgggt tttgctggag gggtatttgt tttcattctg gatctcacca agggtttctt
     4561 cgctgtatat cttgcacgca catatggcaa ttcagaatgg gtggctccct taagcgctgc
     4621 cctggttgtt atcggtcact gttggcctgt gacggctggt tttcgtggcg gaatgggctt
     4681 ggccacctct ggtggggcta tattggcagt aaactttata tggtttatga tcggggtggg
     4741 attattggtc gtgctggttt tgttaattcg tcatagcgcc cgagccagtg tgtttgcaag
     4801 cttaagttta ccgtggggtg tgtggattgc tggattccga ggtgaactat tttggaccac
     4861 tttgtgcatt ggcattgtcg tagcgattcg ttttttggat gattggaatc gtgaataccg
     4921 tgagctatgg cttgatcgcc cggattaatt tcaacaaatt cttggcagca actcgccgct
     4981 tagcgtatcg acgatgcgcg tgctaccgat ggatgttttc attagaacgc gctcttgggg
     5041 ggcttcaatt acctcgccga tgatgcaagt ctgtgtgcca taagcctgtt tgcgcatagc
     5101 cacaatcacg ttgtctacat cctctttgcc aataattgca atcagcttcc cttcgttggc
     5161 aacatacaag gggtcgaagc caagcatctc gcaagctgct tggactgctg ggcgaacagg
     5221 gatgctggat tcatctagca taattccaac cttggactgg cgcgcgatct cattcaggct
     5281 ggtggctaat cctccgcggg tgggatcgcg taaaacatgt accctatcac tgacttccaa
     5341 aatggaagct actaatttat tgagtggagc tacgtcgctc attatctccg cttcgaagcc
     5401 aagttcgcca cgtgctgcta gaacagcaat tccatgatcg ccaatcgggc cggagaggat
     5461 cactttatcc ccagggcgag cgtgctctcc accgatctcc acgccaggca tgaccaagcc
     5521 aataccgcta gtgttgatat agaggccatc tgctttgcct ttttctacaa ctttggtgtc
     5581 cccagcga
//
LOCUS       DBNGSX010000312         4112 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_579669, whole genome shotgun sequence.
ACCESSION   DBNGSX010000312 DBNGSX010000000
VERSION     DBNGSX010000312.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4112)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_579669"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(406..1479)
                     /locus_tag="AC16CB_09455"
     CDS             complement(406..1479)
                     /locus_tag="AC16CB_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342734.1"
                     /translation="MYTSMGGMEICPQCGYGSPSAQRKKEAQQAAQLVRNFVGWKLLI
                     SAPEGSMLTVNNREFVLGLDKLYRDAMKGFEENKLLPCAEKVATTLDVQPAQVPIEGY
                     YTESRALTRYFRLMRALQEIDEARENVLKHLPEFQLLWDVTSSPLYGRPQRNNKLLPV
                     GRDPLSQALRDTRPDWSVERLVKAAYDAALRYEDFSLLGLAARTQDAVVLTATRESTV
                     LYAEFISFGFHVEPEFRYEWRVDEALAEAANRFIETFNNFVPGGLPKAEASNAEKFYK
                     AYTDNEIIGRCVRVGQTNGSPYYHWAIVIKPFSQDEYEFVVDEFWSENIWTTENYRKA
                     QGSPASMKLFQVDGVPGEEEELL"
     gene            complement(1639..2637)
                     /locus_tag="AC16CB_09460"
     CDS             complement(1639..2637)
                     /locus_tag="AC16CB_09460"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3342735.1"
                     /translation="MSPKLNFTHRRKNLVMLIAVIILILATALILFRPVDAQNFISRP
                     NPVQNYNQAEQRIQALQSEETDQLNPVCRTQFLSYGKKVERVIVFIHGNTTCPQQFAE
                     LGKLFYQNGYNVLIAPLPRHGLADRMTEAQAQLTAEHLATYGDKVVDIAQGLGDHVTL
                     AGLSAGGVVTGWAAQNREDIDVAVLISPGFGFAQVPPALTLPAANLYSIIPNSFEWWD
                     PTLKENIEPSYAYPRYSTRALAQVLRLSLAVQSQASQSGPKAQTVWVVTNANDSVVSY
                     PLIQHVVSLWEQQGTDRVRTYQFPPELHLGHDLIDPNQPDQRIDIVYPKLMEIIAK"
     gene            complement(3026..4057)
                     /locus_tag="AC16CB_09465"
     CDS             complement(3026..4057)
                     /locus_tag="AC16CB_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HIR3342736.1"
                     /translation="MNAYLGVDVGSVTTKLVVLDNDYNLLSSLYLRTRGKPIDAVQQG
                     LCEIAERLEGMSIEINGVGTTGSGRYLAAVVIGADIIKNEITAHAVATSYYVPGVQTI
                     VEIGGQDSKIIILRDSVAVDFGMNTVCAAGTGAFLDQQAARLNIKVEDIGLLALQSKT
                     PVRIAGRCTVFAESDMIHKQQMGHRVEDILYGLCQAMSRNYLNNVGLGKEVREQVAFQ
                     GGVAFNQGMIRAFEEALDVSIHVPPHHEVMGAIGAAMLAREAMLYSTQTTRFIGFSAS
                     QMDYRTTSFECRFCSNRCEIVQVSVNKHVVARWGGRCDRWDVNDAIINRPTASIAVAM
                     PSKESCEVC"
BASE COUNT         1009 a         1075 c          972 g         1056 t
ORIGIN      
        1 gtcttggggt tcttgtaacc attgaccaat caatagtaat catgattgca cctccctaaa
       61 tgattttgat tgtatttaga agcctttgtc tattatagta caaaatttct tagaatgtgg
      121 ctattgaaca atttggaaca gattgagatc ccggtgaggc agatttgcac aactgcttgg
      181 aattaatcca cgaatccttg aaaattaagc cagttttcgc agggagagga aaccccctat
      241 ccccgcccgc tttattatca aggtgtaatt gcggctgggg atgggtgatg ctatcctgct
      301 atctcttcgc cctcctcaag gtaaactcta gcctccaatc agcaagcgca aatcgcacgc
      361 cgcctaaata aagaagaagc ccacaaaccc ataatgcgcc tcgttttata acagctcctc
      421 ctcttcaccg gggacaccat ccacctgaaa caacttcatg ctggctggag agccctgggc
      481 tttgcgataa ttctccgtcg tccagatgtt ttcactccag aactcatcca caacgaattc
      541 atattcatcc tgggagaatg gttttataac gatagcccaa tgatagtaag gagagccgtt
      601 ggtttggcct actctcacac accgcccaat aatttcgttg tcggtgtagg ctttgtaaaa
      661 tttttctgcg ttgctggcct ctgctttagg tagtccgcca ggtacaaagt tgttgaaggt
      721 ttctatgaaa cgattggccg cttcggccag agcttcgtct acgcgccatt cgtatctaaa
      781 ttccggctct acatgaaagc caaaggatat aaattctgcg tagagcacgg ttgactcgcg
      841 cgtggcggtg agaacaacgg catcctgtgt gcgtgctgcc aagcccagca acgagaagtc
      901 ttcatatcga agagcagcat cgtaagccgc cttgaccaat ctctcaacgc tccaatctgg
      961 tctcgtatcc cttagggctt gcgaaagcgg atcgcggcca actggaagaa gcttgttatt
     1021 ccgctgcggt cgaccgtata gcggagaact ggtcacatcc catagcaatt ggaactctgg
     1081 taggtgtttg agtacatttt ctctagcctc atctatttcc tgcaaggcac gcattaaccg
     1141 gaagtatctc gtcagggcac ggctctcggt atagtatccc tcaatcggaa cctgcgccgg
     1201 ctggacatcc aacgttgtag ccactttttc agcacagggg agcagtttat tttcctcaaa
     1261 ccctttcatc gcatcacgat acagtttgtc taaacccagc acaaactcac gattgttcac
     1321 ggtcagcatg cttccctcag gggctgagat taggagtttc cacccaacaa agtttcggac
     1381 gagttgtgct gcttgctgtg cttctttttt tcgctgtgcg ctaggcgagc cgtaaccaca
     1441 ttgagggcaa atttccatgc cacccatgga tgtgtacata attgttccac agtcaggaca
     1501 attcattttt caccttcatt tctggtcgtc ttatgccttt ttactttaag ttgcccaaca
     1561 ttggactact gacaggttac acgcccggac ttgagcttgt tatcaaaaga tagcttttta
     1621 tgacatacgg gtaactggct attttgcaat gatctccatc agctttgggt agactatatc
     1681 aatgcgctga tccggttgat tcggatcaat gagatcatgt cccaagtgta actctggcgg
     1741 aaattgatag gtgcgcaccc ggtcagtccc ctgctgttcc cataacgaca cgacatgctg
     1801 tatcagggga taactcacca cactatcgtt ggcgtttgtc accacccaaa ccgtctgcgc
     1861 cttggggcca ctctggctcg cctgtgactg cacggctaaa ctcaaccgta atacctgggc
     1921 caaagcgcgg gtagaatacc gtgggtatgc ataggaaggt tcaatgttct ctttaagggt
     1981 tggatcccac cactcaaatg aatttggtat gattgagtag agattcgcgg ctggtagtgt
     2041 tagcgcgggc ggtacctgag caaatccaaa gccgggtgaa atcaggacag ccacatcgat
     2101 gtcctctcta ttctgcgccg cccagcccgt aaccacgcca cccgcggaca agcctgccag
     2161 ggttacgtga tcccctagcc cctgtgcgat gtctaccact ttatccccgt aggtagctaa
     2221 atgctcggca gtcagttgag cttgcgcctc ggtcatgcga tcggcaaggc catgcctggg
     2281 caggggcgca ataaggacgt tatatccatt ttggtagaac agcttaccca actcggcaaa
     2341 ctgctgtggg caggttgtgt tgccatggat aaaaacgatg acccgctcga cctttttgcc
     2401 gtacgacagg aactgtgtcc ggcagaccgg gttgagctgg tctgtctcct ctgactggag
     2461 ggcctggatt ctctgctcag cctggttgta attttgaaca ggattggggc gcgaaatgaa
     2521 attttgtgca tctacaggtc ggaacaagat aagcgcggtc gctagaataa ggatgataac
     2581 cgcgatcaac atgaccaaat tcttcctccg gtgcgtgaag ttcaatttcg gactcactcc
     2641 gacctcccca ggattgtaac tctgtttaat tggcaaagca ggtattagca atgtgtaatg
     2701 cacacctaga atactgcccg gtggttagca ttagcggcaa tgggctggca gagatctgcc
     2761 gtctccgtgg aaaaaaactc ccagcagcga cgaatttagc gcgcccacaa tcacccacgc
     2821 aacacgaaac gctgtgagca tcgtcattcc cgcgatcaat accaggagcc atttcaaggc
     2881 tcggaaattt ttcgctagag ttatatcact ctgcataagt tatctcccac atgtcaagtt
     2941 gtggctcgcc caagcgaggc ggagagtgga ataatgattg gtatgtcatc caagagttaa
     3001 gctgaggctc agtttatcaa cagcattaac aaacctcgca agattctttt gatggcatgg
     3061 ccacagctat cgatgcagtc gggcggttga taatggcatc gtttacatcc cagcgatcac
     3121 agcggcctcc ccagcgtgca acaacatgtt tgtttacgga cacctgcacg atctcacacc
     3181 ggttcgaaca gaaccggcac tcaaaggagg tggtgcggta atccatttga ctggcactaa
     3241 acccaatgaa gcgggtggtt tgggttgaat atagcatcgc ttcacgcgcc agcattgccg
     3301 cgccaattgc tcccatcact tcgtgatgcg gaggaacgtg aatggaaaca tccaaagctt
     3361 cctcgaaagc acgtatcatc ccctggttaa aggccacgcc accttggaat gcgacctgtt
     3421 cacgaacttc tttccccagc ccaacgttgt tcaaatagtt acggctcata gcctggcaaa
     3481 ggccatacag gatatcctcg acgcgatgcc ccatctgctg cttgtggatc atgtcggatt
     3541 ctgcaaatac ggtacaacgt cccgcaatac gcaccggcgt tttggattgc aaggctagca
     3601 gccctatatc ttcgactttg atgttcaggc gtgcagcctg ttgatccagg aatgcgccgg
     3661 tacctgcagc gcacaccgtg ttcattccaa aatcgaccgc aacgctatcc ctcaagatga
     3721 taatcttgga atcttggccg ccaatctcaa cgatcgtttg tacgccagga acataataag
     3781 acgttgccac tgcatgagcg gtgatttcat tcttgataat atctgcgccg atcactacag
     3841 cagccagata tcgcccgctg ccggtagtac ctacgccgtt gatctcaatg gacataccct
     3901 caaggcgttc tgcaatttca caaagaccct gttgaacggc atcgatgggt ttaccgcgcg
     3961 tgcgcagata gagtgatgac aataagttgt aatcattgtc aagaacaacc aacttggttg
     4021 tcactgagcc tacatctaca cctaaatatg cgttcatagc gttctccttg aaaagtcacg
     4081 ctggctgttc agattacagt gggagagcct tg
//
LOCUS       DBNGSX010000313         2404 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_95567, whole genome shotgun sequence.
ACCESSION   DBNGSX010000313 DBNGSX010000000
VERSION     DBNGSX010000313.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_95567"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="AC16CB_09470"
     CDS             complement(<1..203)
                     /locus_tag="AC16CB_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012610.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3342737.1"
                     /translation="MTTPSNLNPQGPVPLYIQLRDSLLRLIRQGDLKPHDRLPSEREL
                     GVEYGISRMTVRQAIQALIRDGLL"
     gene            627..>2404
                     /locus_tag="AC16CB_09475"
     CDS             627..>2404
                     /locus_tag="AC16CB_09475"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR3342738.1"
                     /translation="MTIESALETSEEAPDLGLSPQEILSDYRLAYRSRQVSLIARREV
                     MSGKAKFGIFGDGKEIVQLAMAKVFRKGDWRSGYYRDQTWMFAVGITSIQEFFAQLYA
                     HTDVDADPATAGRAMNAHFGTRSLNPDGSWKDLTQMLNSSVDISPTAAQMPRLVGLAY
                     ASRLFRELPELEGFNQLSQGGSEIAFGAIGNASCAEGLFWESLNAIGVLGAPALITIY
                     DDGYGISVPNEQQIIKTNISELLQGFQRDPERCGGYDNCGYDIYTVPGWDYPRLVQTY
                     RKAEASVRDEHVPAIVHVNELTQPQGHSTSGSHERYKTNERLEWEREFDGLKKFREWI
                     IAQDIASADVLDEMDAEDRGQVEEIRKQAWQTYIAPIEGERAELVRLIKQMAKGSSQA
                     SELSALQRKLINTPNPLRRDLMVATREALMAVRGEQIPAKQALIAWKKAQQPVQEERY
                     SSHLYSQSAESPLRVAEVQPVYSDESPSVFGFEVLNAFFDTALGRDPRVIAFGEDVGR
                     LGDVNQGMRGLQEKHGVFRVSDTGIREATIIGQAIGMALRGLRPIAEVQYLDYVLYAL
                     QILSDDLATLHWRTKGGQKAPVILRTR"
BASE COUNT          557 a          596 c          739 g          512 t
ORIGIN      
        1 aggaggccat ctcggataag agcctggata gcctggcgga cagtcatgcg actgatgccg
       61 tactctactc caagctcacg ctcagagggc aggcgatcat gaggcttgag atcgccctgg
      121 cggatcaagc gtagcaacga atctctgagc tggatgtaga gcgggacagg accttgcggg
      181 ttgagattgg aaggagtggt cataagtggt atataccaga ttgtagacaa aaaaattatc
      241 tatgtcaata gtttttttct ggatggcgga tggatttggg acgcgggagg tgtcaagtag
      301 catgaaccat atgcgaagta catcatgcga cctgcgataa gcgaatttca cacaaggggg
      361 agattgttac ctcgagatat tcggggcggc tattcgtaac accaagagta caaattaaaa
      421 actctttaca ctttgcacct ccttcgtgtc ctttgtgttt aattattact ttgtgcaact
      481 ggcggcttct ttctcaggac aaggctgcag caaatttata aaacactcac cgaacaagcc
      541 caaaaaggga gatataatct taatcaccca atcgttggct gtgcgggttt ggtttagatg
      601 gggaaaggtt tttaggaggt attgcgatga cgatcgagtc agcgcttgag acaagtgagg
      661 aggcgcctga cctcgggctt agcccacagg agatattaag tgattaccgg ctggcatatc
      721 gcagccggca ggtcagcctg atcgcacggc gggaggtgat gagcggcaag gccaagtttg
      781 gcattttcgg ggatggtaaa gagatcgtgc aactggcgat ggccaaggtt tttcgtaagg
      841 gggattggcg ctcgggatac taccgcgacc agacctggat gtttgccgtg ggtatcacca
      901 gcatccagga attttttgcc cagctatatg cccatacgga tgtggacgcc gaccccgcca
      961 cagcgggacg ggcgatgaat gcccactttg gaacgcgcag cttgaatcca gatgggagct
     1021 ggaaagacct cacgcaaatg ctgaactcgt cggtagatat ctcgcccact gctgcgcaga
     1081 tgcccaggct ggtcggtctg gcgtatgcct ctaggctgtt tcgtgagctg cccgaactgg
     1141 aaggcttcaa ccaattatcc cagggaggca gcgagatcgc cttcggggcg attgggaatg
     1201 cttcctgtgc ggagggcttg ttctgggaat cgctaaatgc catcggcgtg ctgggcgcgc
     1261 cagcgttgat caccatctac gatgatgggt acggcatctc ggtgccaaac gagcagcaga
     1321 tcatcaagac caatatctct gagctgttac aaggtttcca acgcgatccc gaacgttgtg
     1381 gggggtatga caattgtggt tacgatatct atacggtgcc gggttgggat taccctcgcc
     1441 tggtgcagac ctaccgcaaa gctgaagcaa gcgtgcggga tgaacatgtg ccagccatcg
     1501 tgcacgtcaa cgaactgacc cagccgcaag gacactccac gtcgggaagc cacgagcgct
     1561 ataagaccaa tgagcgcctg gagtgggagc gggagttcga cgggctaaag aaatttcgcg
     1621 agtggatcat tgctcaggac attgcgtctg ccgatgtgtt ggatgagatg gatgccgaag
     1681 accggggcca ggttgaggaa atccgcaagc aggcttggca gacgtacatc gcgcccattg
     1741 agggggagcg ggctgaattg gttcgcctaa ttaagcaaat ggcaaaggga tcgagccagg
     1801 cgagtgagtt gagtgcgctc caacgcaagc taatcaatac ccccaatccc cttcgtcggg
     1861 acctcatggt ggctacacgc gaagcgttaa tggcagtgcg cggcgaacag atccccgcca
     1921 aacaggcgct gatcgcctgg aagaaagctc agcagcctgt gcaagaggag cgttatagct
     1981 cgcatttgta cagccaatcg gctgaatcgc ccttgcgggt ggcggaagtc cagccagtat
     2041 attcggacga atcgccatcc gtgttcggct tcgaggtgct caacgccttc ttcgacaccg
     2101 ccctggggcg cgacccgcgt gtgatcgcct ttggcgagga tgttggtcga ctgggcgacg
     2161 ttaaccaggg gatgcgcggc ctgcaggaga agcacggcgt cttccgcgtg agcgataccg
     2221 gcatccgcga ggctactatt atcgggcaag ctatcggcat ggcattgcgc gggctgcgcc
     2281 ctatcgctga ggtgcagtat ctggactacg tgctgtacgc cttgcaaatc ctatccgatg
     2341 acctggcgac cctgcactgg cgcacgaaag gcgggcagaa ggcgccggtg atcctgcgca
     2401 cgcg
//
LOCUS       DBNGSX010000314         1634 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1896354, whole genome shotgun sequence.
ACCESSION   DBNGSX010000314 DBNGSX010000000
VERSION     DBNGSX010000314.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1896354"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1114)
                     /locus_tag="AC16CB_09480"
     CDS             complement(161..1114)
                     /locus_tag="AC16CB_09480"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family RNA-guided transposase"
                     /protein_id="HIR3342739.1"
                     /translation="MTTPSEAYVGIDVGKTWLDIARWGSETVWRVSNDDDGVAKIMAW
                     VAEMEPQLIAVEATGGYEQLLVQSMLLNNLPVAVINPTRVRALSKATGKLAKTDVIDA
                     RLIAEYAFKIQPETLGPKEANEIRLNALVTRREQLVEMRVAEQNRLGTAQNSMQADIR
                     EHIEWLSSRIEALEAQINALVDSLPAWREQVQRLDSIPGVGRITAVTVLSEMPELGQL
                     DRQKIAALAGLAPFNRDSGHQRGKRRIFGGRKGIRRVLYMACLSAKKYNPVIRSLFER
                     LMKKGKVFKVAITACMRKLLTIMNAMTRDQVNWRTPKPISV"
BASE COUNT          368 a          434 c          424 g          408 t
ORIGIN      
        1 cgtcgagcag ccaagttggg ctttcgcttg cagcccacgt tccaagaagt ttcttaggag
       61 cctgcgtaat ctggcaggcg aagcgatctc atagaaatgt caggagattg ccacgcccct
      121 gcggggctcg cagtaactgt gtttttagta aagatggaat tcatactgaa ataggcttag
      181 gtgtcctcca attgacctga tcgcgggtca tggcattcat gatggtgagc aacttacgca
      241 tacatgcggt gatagcaacc ttgaatactt tgcctttctt catcaaacgc tcaaacaagg
      301 atcgaatgac aggattgtac ttcttagcgg acaggcaagc catatacagt acgcgacgga
      361 tacctttgcg acccccgaag atgcgccgct taccgcgctg gtggccacta tcccggttga
      421 agggggccag gccagccaag gcagcaatct tctggcggtc cagctggccc aactccggca
      481 tttcggacaa caccgttacg gcagtgatcc tgcccacgcc cggaatgctg tccaggcgct
      541 gcacttgttc tcgccatgca ggcagactgt caaccagcgc gttgatctgg gcttcgagcg
      601 cctcgatgcg tgaactcagc cactcgatat gttcgcggat atcggcttgc atgctgtttt
      661 gggcggttcc caggcgattt tgctcggcaa cccgcatctc taccagctgt tctcgccggg
      721 tgaccaacgc attgagacgg atttcattgg cttctttcgg cccaagggtc tcaggctgga
      781 tcttgaaggc atattcagcg atcagccgcg catcgatcac gtccgttttg gccagcttgc
      841 cggtggcttt ggataatgcc ctcacccgcg tgggattgat caccgcgacg ggcaggttgt
      901 tcaagagcat gctctggacg agcaattgct catacccgcc agtggcttcc acggcgatca
      961 gctggggttc catctctgca acccaagcca tgattttggc gacgccatca tcatcattgg
     1021 atacccgcca gactgtttcg ctgccccacc gggcgatatc caaccaggtc ttgccgacat
     1081 cgatcccaac atatgcttca ctcggtgttg tcattggtac gattcctccg tataatcgag
     1141 atgagcccac actagcaagg attcggcctc tctcggccac tcaactgttc gggctcgctt
     1201 ggatgttgga catggcggtc gtactgcgag acggtctaca ggacctaatg actagcggcc
     1261 tgccatgtcc acttttatta tcatactaat gaccgtcaga ggataaattg tccgaacttt
     1321 tgtctcaact tcagagaaga gctttacata ttgtcgtgca ttttgtatac tgattttaat
     1381 atagattggt cacagattag cacagaagag ctaatcgatc agtcaaatat tttttataag
     1441 cgtgtcatcg ctagcgatag ttagcgtggt ggcgatccct catagcgtga tacctgtaag
     1501 cttaacagag actgcttaag aattatgaaa tcaacaacag tcagcaacgt gaaggtggtg
     1561 tatgctatag gcgagcaggg tacagcggac atcattcgcg gcacctgtga gaaggctatc
     1621 cagctggctt ggga
//
LOCUS       DBNGSX010000315         3161 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_927131, whole genome shotgun sequence.
ACCESSION   DBNGSX010000315 DBNGSX010000000
VERSION     DBNGSX010000315.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_927131"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="AC16CB_09485"
     CDS             complement(<1..465)
                     /locus_tag="AC16CB_09485"
                     /inference="COORDINATES: protein motif:HMM:NF017999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3342740.1"
                     /translation="MKIEGEYLFNGSREDVWEIVRDPQVLATALPGTKSLDKVGENEY
                     AGEMNVRVGPVAGLFSGRLIVSNEVEPESCTLTVDGRGSPGFMNGTGNVFLYDQGDST
                     TLMKYDGEVQIGGKLASVGQRLLDTASKSIIRQGLEALNLALQARVAAKTEGK"
     gene            complement(477..2810)
                     /locus_tag="AC16CB_09490"
     CDS             complement(477..2810)
                     /locus_tag="AC16CB_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HIR3342741.1"
                     /translation="MTKKFIGKRIKRTEDPRLLTGQALFVDDVDIPGMLHVAFLRSDY
                     AHARLKSIDISAAQKKPGVVAVYTAEEMGDEWKPGPPLVSPPPTLQDVLFHSRTQVPL
                     VKDKVRHAGEAIAAVVAESRYIAEDAVEEILVDLEPLEAVADLEKALQPGNVRVHDDL
                     ESNLAAQLVQVKGDYDKARQEADLVIKKRFIIDRGAAAAMENRGVVAHWDAKSQHLTV
                     WDTTQAPIPIRNGLVTRLGLSQHQVRVIAPFLGGGFGPKMMMFYPEEMLIPWIAIKLA
                     RPIKWIEDRRENFYATTQERVQIHDAEMSLTREGRILGIKDNFLHDTGAYDPYGLTIP
                     LNTQSHSMGAYDVPNYYSEIKVVFTNKIIATPVRGAGRPQGIYIAERLLDLAAKELGI
                     DAIEIRKRNFIPPEKFPYEHKIIDQAFSPLIFDSGNYLPVLEKAQNMIGYDQFIQEEQ
                     PRLRSEGRHAGIGIVSFVETTGVGPYEGARITVGTDGKVNVATGVGTQGQGHFTSFAQ
                     IVADQLGVDVQDIIIVTGDTAEFHWGTGTFASRGAVVAGNAIHASAVVVRKKILKLAS
                     EVLKAPEDELELDDGQVRVADMPRMSISLGELAALANPLRGAVQPGTEPGLEATAYYG
                     PQYGATAFGVHAMILEVDPETMMVDIKRYVVVEDCGTILNPLILEGQIHGGVAMGIGN
                     AYYEKLVYDENAQLLNASLADYLIPTATEVPRIEVEHEETLSPLNTLGSKGAGEAGAI
                     PVPALFAQALENALPEYNLEILETPLSPNRLFELLQD"
     gene            complement(2874..>3161)
                     /locus_tag="AC16CB_09495"
     CDS             complement(2874..>3161)
                     /locus_tag="AC16CB_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3342742.1"
                     /translation="FAIQADGHEITTIEGLGTIDNLHPIQESFVEAHGLQCGFCTPGI
                     ILTMIPFLEKNPAPNEQEIRHAISGNICRCTGYQHIVDAVKLAAEKMSAQT"
BASE COUNT          717 a          855 c          744 g          845 t
ORIGIN      
        1 ctttccttcg gttttagcag caacacgcgc ttgcaatgcc aggtttaaag cttctaaccc
       61 ctggcgaatt atgctcttgc tggctgtatc cagcaatcgt tgaccgacac tggccagttt
      121 tcctccaatt tggacctctc catcgtactt catcaaagtg gtgctgtcgc cctggtcgta
      181 taggaacaca ttaccggttc cattcataaa cccagggcta cctcgaccat ctacagtaag
      241 ggtgcagctc tcaggctcaa cctcattgga gacgatcaat cgccccgaga aaagcccggc
      301 aacaggtcca acccgtacat tcatctctcc ggcatactca ttttcgccga ccttatccag
      361 gcttttggtt cccggcaaag cggtagccag cacttgtgga tcccggacaa tttcccaaac
      421 atcttcccgg ctaccattga agagatactc gccttcaatt ttcatctagc tttctcctaa
      481 tcttgaagca gctcgaaaag gcgatttgga ctaaggggtg tttcgagtat ttcaagatta
      541 tattcaggta acgcgttttc caaggcttgc gcaaaaaggg ctggcactgg gatcgcgcca
      601 gcttcgcctg cgcctttact gcccaatgtg ttcagaggtg agagggtttc ttcatgttca
      661 acctctatcc gcggtacttc cgtagccgtg ggaatcaaat aatctgccag ggatgcgttg
      721 agcagctggg cattttcgtc ataaaccagt ttttcatagt acgcattgcc aatccccatc
      781 gcaacaccac cgtggatctg cccttccagg atcaatgggt ttaatatcgt gccgcaatct
      841 tctacgacaa catatcgctt gatgtccacc atcatcgtct ctggatctac ttcaaggatc
      901 atagcatgaa cgccaaaggc ggttgcgcca tactggggtc catagtatgc cgtggcctct
      961 aaacccggtt cagtgcccgg ctggactgcg ccgcgcaatg gatttgccaa ggcagccaat
     1021 tcccctaagc tgatagacat gcgcggcatg tcagccaccc gtacctggcc gtcgtccagt
     1081 tccagttcat cctcaggcgc tttgaggact tcgctcgcta atttgagtat ttttttgcgc
     1141 actactactg cagaggcgtg gatggcattc cctgctacga cagccccacg gctggcgaac
     1201 gtaccggtcc cccaatgaaa ttcagccgta tcgccagtaa ctataataat atcctgaaca
     1261 tctactccta attgatcggc aacaatttga gcaaaggaag tgaaatgccc ctgcccttgt
     1321 gtgccgacac ccgttgccac attgactttg ccatcggtcc caaccgtaat tcttgcgccc
     1381 tcatatggac ccacaccagt tgtctcaacg aaggatacga tcccgatccc tgcatggcga
     1441 ccctcggagc gcaagcgtgg ttgctcctct tgtatgaatt gatcatagcc aatcatattc
     1501 tgagcttttt ccagaacggg caggtaattc ccgctatcga agatgagcgg agagaaagcc
     1561 tggtcaatga tcttgtgttc atacgggaat ttctccgggg gtataaaatt acgctttcgg
     1621 atttcaatcg catctatacc aagctctttg gcagccaggt ccagcaatcg ctctgcaatg
     1681 taaatccctt gaggccttcc ggctccccgt actggcgtgg caatgatttt attcgtaaaa
     1741 acgactttga tctcagagta gtaattggga acatcgtaag cccccatgga gtggctttgg
     1801 gtgttcaggg ggatcgtcaa tccataggga tcatacgcac ctgtatcatg caggaaatta
     1861 tccttgatac ccagtatgcg cccctcgcgg gtgagcgaca tttcggcatc gtgtatctgc
     1921 acacgctctt gggtggtggc atagaagttt tcccgccgat cttcaatcca tttaatcggt
     1981 ctggctaact tgattgcgat ccaggggatg agcatttcct ccgggtaaaa catcatcatt
     2041 tttggaccaa acccgccgcc caggaacgga gcaatcaccc gcacttgatg ctgtgataaa
     2101 ccaagcctgg tgacaagacc attccgaatt ggtattgggg cctgggttgt gtcccatacc
     2161 gttaagtgct ggctttttgc atcccagtgg gcgacgacgc ctcgattttc catcgcagcc
     2221 gcggctccac ggtcgatgat aaaacgcttc ttgatgacca ggtcagcctc ttgtcgggct
     2281 ttgtcataat cgccttttac ctgtaccaac tgggcagcca ggttcgattc gagatcgtcg
     2341 tggacgcgca cgtttcccgg ctgtaaggct ttttctaagt ctgccacggc ttctaagggt
     2401 tcaagatcaa ccaggatttc ttctactgca tcttcagcga tgtagcggct ttcagcaact
     2461 accgccgcaa tggcttctcc tgcatgcctg accttatctt tgaccagagg cacttgcgtt
     2521 cgagaatgga ataacacatc ttgcaatgtc ggtggtggag atactaatgg aggtccgggt
     2581 ttccactcat cccccatttc ttcagcggtg taaactgcaa caacgccggg tttcttctgc
     2641 gcggcagaga tatcaatgct tttcaggcgc gcatgggcat aatcgctccg taagaatgcc
     2701 acatgtaaca tacctggaat atcaacatca tcgacaaata aggcctgccc ggttaataga
     2761 cgtgggtctt cggttcgctt tattcgtttg cctataaatt tcttggtcac ggatcactcc
     2821 taaacataat ccatctaatc atgtatatcg agtcgctcta ggtgatgtgt tgctcaagtc
     2881 tgggctgaca tcttctccgc tgcaagcttc acagcgtcta cgatgtgttg atagccagta
     2941 cagcggcaga tatttccaga gatggcatgg cgaatttcct gttcgtttgg agcaggattt
     3001 ttttccagga acgggatcat cgtaagaatg atcccgggtg tacaaaagcc gcactgcaaa
     3061 ccgtgggcct ccacgaagct ttcttgaatg ggatgcaggt tatcgatggt tcctaagcct
     3121 tcgattgtag tgatttcatg cccatcggct tgaatggcga a
//
LOCUS       DBNGSX010000316         2965 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_164594, whole genome shotgun sequence.
ACCESSION   DBNGSX010000316 DBNGSX010000000
VERSION     DBNGSX010000316.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_164594"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(317..1576)
                     /locus_tag="AC16CB_09500"
     CDS             complement(317..1576)
                     /locus_tag="AC16CB_09500"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIR3342743.1"
                     /translation="MIMNEIGKKGRTAARSMAHASQEQKRAVLLRLADQLVEASTEVL
                     SENQIDLQAARGVGLSDAMLDRLMLNEGRIHGIATDLRAVADLPDPVGEVFDEQTLSN
                     GLRVHKQRIPLGVLGVIYEARPNVTMDVAGLAIKTGNAAILRGGSETINSNRILVSVI
                     QRALLENGLPPEVIQFVDDPDRARVYELLQLHEYVDMIIPRGGAALHDFCRENSRIPV
                     ITGGIGICHLFVDESADLEAGLQVIRNAKIQRPTVCNALDTALVHRNVADQFLPKLVV
                     YLLQDGVSFRAHDSALPYLDQTTHESVKSAGPGDFDIEWLSLVLGLKVVDGLDEAIAH
                     IGEHSTGHSDGILTRDNENARRFVEQVDSAAVYVNASTRFTDGGQLGLGAEVAVSTQR
                     LHARGPMGLRELTTYKWVIIGDNHIRM"
     gene            complement(1573..2697)
                     /gene="proB"
                     /locus_tag="AC16CB_09505"
     CDS             complement(1573..2697)
                     /gene="proB"
                     /locus_tag="AC16CB_09505"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIR3342744.1"
                     /translation="MQVEKIMNFQRIVIKLGTSTLTAGTPGISPPRLIELVRQMAELY
                     RPGRELLLVTSGAIAAGREWLKFPDLPKDIPIKQMLAAVGQPRLMALYEQLFRMYGIT
                     VGQVLLTRTDLANRRGYLNARNTLAALLSQGVIPIINENDTVATEEIRVGDNDTLSAL
                     VANLIEADLLVILTDQPGLLTADPRKDPSAQLVTEIREAEIPEALWQAAGGSTSRLGT
                     GGMITKLQAADLARRSGAYVFIARGSDADVLLRLVNGEALGTRFLPLATSVESRKRFL
                     LTGGRSRDRLVVDTGASQALHAGGSLLPVGVTSVEGGFERGDPVRVLDQQGREIARGL
                     VNYSAADLARVCGRHSSEIEKILGYMYGEEVIHRNNMVLL"
BASE COUNT          686 a          891 c          738 g          650 t
ORIGIN      
        1 ttcagttact tcgctcattt tttctccttt ctatcaaaca tgctttgact tgataatact
       61 attggcggaa gcaggttgcc caaaaagtgt atataacgca actattcggt taattcggat
      121 ataaatcatc ctaatagaaa ttatacaccc taaatgccac acttccaccg aaaaaagcag
      181 ggcaattgca tatgccctga ggggcatgcg caatcccgca aaagagacct caaccaggtc
      241 cgcgaccggt tgagcatgaa gagctatgcc tagttgtagt attagcctgt caaaaggagc
      301 tacgtgcgaa tcgagttcac atgcggatgt gattgtcacc aataataacc catttatagg
      361 tggtcaattc gcgtaaaccc atcggacctc gagcgtgcaa gcgttgcgtg gatacagcca
      421 cctcagcgcc caatcccagc tgcccaccgt ccgtgaaacg agtgcttgca tttacataca
      481 ccgcagccga atcgacctgt tccacaaacc ggcgcgcgtt ttcattatct cttgtcagaa
      541 taccatccga gtgaccggtg ctatgctcgc cgatgtgagc aatagcttca tcaaggccat
      601 caaccacctt caaccccaaa acaagtgaga gccattcgat gtcgaaatct ccaggaccag
      661 ccgattttac gctctcatgt gtggtttgat ccaggtaagg caatgccgag tcatgcgccc
      721 gaaaggaaac accgtcttgc aacagataga caaccagctt gggtaagaat tgatcggcga
      781 cattacgatg caccaaggcc gtatccagtg cgttgcatac tgttggtcgc tgtatcttgg
      841 cattccggat cacttgtaaa ccggcttcta aatcagcgct ctcatctacg aacaagtgac
      901 agataccgat acccccagtg ataaccggga tgcggctgtt ctcccggcaa aaatcgtgca
      961 acgcggcccc gccgcgtggg atgatcatat ccacgtactc gtggagttgc agcaattcgt
     1021 acacgcgcgc ccggtctgga tcatccacaa actgaatcac ttcaggcggc aagccatttt
     1081 ccaataaagc gcgctgaatc actgacacca ggatgcgatt agagttaatc gtctcactgc
     1141 cgccgcgcaa tattgctgcg ttaccggtct tgatcgccaa tccagctaca tccatggtca
     1201 cgttcgggcg ggcttcatag atgaccccca atacgcccag tgggatgcgt tgtttgtgga
     1261 ctcgcagccc gttggagagc gtctgctcgt caaatacctc gcctaccgga tccggtaagt
     1321 ctgccaccgc ccgcagatct gtggcgatac cgtgtatgcg cccttcgttt aacatcagcc
     1381 gatccagcat cgcatcgctc aagccgaccc cacgggctgc ctgcaggtct atctggtttt
     1441 ctgacaagac ctccgtcgag gcttccacca attgatcagc caggcgtagc aagaccgccc
     1501 tcttttgctc ttgcgaggcg tgagccatcg agcgggcggc agtgcgcccc ttctttccga
     1561 tttcattcat gatcataaca gcaccatatt attccgatgg atgacttctt caccatacat
     1621 ataacccagg atcttctcaa tctcgctgga gtgtcgcccg cagacgcgcg caaggtcagc
     1681 cgccgagtaa ttgaccaggc cacgggcgat ctcccgccct tgctgatcca gcacacgcac
     1741 cgggtcaccg cgctcaaaac caccttccac cgaggtcacg ccgacaggca gcaagctacc
     1801 tccagcatgt aatgcctgcg acgcacccgt gtccaccacc aatcgatccc tcgagcgtcc
     1861 gccagttaac aggaaacgtt tgcggctctc gaccgaggta gccagcggga gaaaccgcgt
     1921 gccgagtgcc tctccattca ccaggcgcag caagacatcc gcatcgctgc cacgcgcgat
     1981 gaacacgtat gccccagagc gccgcgccag gtcggcggcc tgcagcttag tgatcatgcc
     2041 acccgtcccc agccggctgg tcgagccacc cgctgcctgc cacaaagcct ccgggatctc
     2101 agcctcccta atttctgtca ccagctgtgc cgagggatcc ttgcgggggt cagcggtgag
     2161 caacccgggc tgatcggtga gtatcaccag gaggtcggcc tcaatcaggt ttgccaccag
     2221 cgcggatagg gtatcattat ccccaacgcg aatctcctcc gtggcgacgg tgtcattctc
     2281 attgatgatg ggtatcacgc cttgggagag cagggctgcc agcgtgttac gtgcgtttag
     2341 ataacctcgc cggttagcca gatcggtgcg tgtcagcaac acctgaccta ccgtgatgcc
     2401 gtacatacga aagagctgtt cgtataaagc catcaagcgc ggctgcccaa ccgccgccag
     2461 catctgcttg atcggtatat cctttggcaa atcagggaac ttcaaccact ctctcccggc
     2521 ggcgatcgct ccagaggtga ccaataacag ctctctgcct ggacggtaca gctcggccat
     2581 ctgccgaacc agctcgatca ggcgcggtgg agagataccc ggcgtaccgg cggtgagcgt
     2641 tgatgtaccc agctttatca caatgcgctg gaaattcatg atcttttcaa cctgcattta
     2701 tcccaaccct gtcggatttg tcgtattata ctatgagctg atacgtattg gacgcttggg
     2761 accccttcaa ccaagctacg cctctgtagg agaaaacatt atgagtaaac ttcttatcct
     2821 tggagctacg aacggactga tcggatgcga atgtaacgac gatggctggc gcgtgtctcg
     2881 gcaaggccaa agcgggctga taggtctgcg ggtcaccagc gctgccgccc gagatggagt
     2941 catgcttgca ggcacacgcg atggc
//
LOCUS       DBNGSX010000317         4845 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1826566, whole genome shotgun sequence.
ACCESSION   DBNGSX010000317 DBNGSX010000000
VERSION     DBNGSX010000317.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4845)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1826566"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="AC16CB_09510"
     CDS             complement(<1..317)
                     /locus_tag="AC16CB_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342745.1"
                     /translation="MKRVLLLVFSIILLGAATLASVGVGASLTSQTYSAGLAIPTGFD
                     KVTAVYGAQLYRKDYQGGNPDFVQVVDLSLGAEIELLHGSIKEKREGHGAYGGDDPRI
                     LSRS"
     gene            complement(674..2287)
                     /locus_tag="AC16CB_09515"
     CDS             complement(674..2287)
                     /locus_tag="AC16CB_09515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR3342746.1"
                     /translation="MSKYDKVIPVVFFLIFVLLTVQGISWGAPGFWHPDEVIQQADKA
                     IERGYRFDEKDFYYPSLPKYVMYVVGKLVYAMGLDKTKFIEAARLVSVFLGGLVVALV
                     YVLTRQAGGGVLAGTLAALFVLSHRDTAIYSRFAHGDIYLAFFACLTSLWLLKYRVKG
                     HRLWLYIAFFSAGLAISSKYNGMGLFLAALFIFLVTNRRGLFENPFTFFEMLFVSTLL
                     SAFGFAIGTPRSILSMEFYFQNAVPAIRRLAIYEIEPDTVAGIFGQWAVLRVSLGTAV
                     SLVFLVALIWHVLKVIRYYAGRHLINDLRMELIAIVLLCLFALDLPVLISYRYPPRYL
                     LSTIPILAVLAGSFVEEALHRIERGQIRYLRELSIVGLAIVIAYSFLRVVAVLLLFLN
                     DNRILASRFLGTLPEGSTLEYTYSPPNIPRGHFSKLYEHPSFDKLYADQPAPGGNSAK
                     YNQGEAGVERRQPDYLLVDSFTYGDFLNQSDCVYRQMECEFYSRLFLGETNYKLIKKF
                     DYQLPPFLPVTRATFVNPVIQIYQRESSP"
     gene            complement(2674..3192)
                     /locus_tag="AC16CB_09520"
     CDS             complement(2674..3192)
                     /locus_tag="AC16CB_09520"
                     /inference="COORDINATES: protein motif:HMM:NF016764.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HIR3342747.1"
                     /translation="MFNRIMGVFKLDVNTFEEVEHDETATSQAAIVVGLVALLSALGS
                     GLGASFSGSNFLTSFIGTFVWAFVGWFLWSGVSYLVGTSLFGGQATLGEMLRVLGFAQ
                     APQLLGIIPCLGGLVGAIWALIAGFIAIRQGLDLDNTKAFLTALIGFVVLMIGYAIIY
                     SMLGIGGALLGI"
     gene            3487..4398
                     /locus_tag="AC16CB_09525"
     CDS             3487..4398
                     /locus_tag="AC16CB_09525"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR3342748.1"
                     /translation="MSNKSNPLTALSDAMTEAVAKAGAATVLVNARRRFPASGIAFTP
                     DLILTADHVVERDEDILVVLPDGSQVKATIAGRDSGSDLALLRLERAAASSAEPASQD
                     PRVGQIALSLGRPTQEGIQASLGIVSAIGGPVRTGRGGLLDRYLRTDAIPYPGFSGGP
                     LVDTAGRVIGVNTSGLTRGASLAIPINLAWQTAETLARHGRVRRGFLGVRSQPVSIPT
                     SQQKALGRDQSSGLLLVGVEEKSPADVGGLLVGDIIVAIAGQSVTDADQLLSSLAGDV
                     VGKPTAVDVLRGGQPVTITVTIGERKD"
     gene            4401..>4845
                     /locus_tag="AC16CB_09530"
     CDS             4401..>4845
                     /locus_tag="AC16CB_09530"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR3342749.1"
                     /translation="MDVQTLSNQIADALAEVALRVQRSLVVVHNGRHGAGAGVVWRQG
                     GYIITNHHVIARGRIAVTLPDRGELPARVVAKEPQLDLAILQVQNDSLAPARIADSRV
                     LQVGQIVLAVGHPWGQRGVVTAGIISGLGTAQTRDQRQSVPIIRSD"
BASE COUNT         1214 a         1236 c         1312 g         1083 t
ORIGIN      
        1 gaacgggaaa gaatgcgcgg gtcgtcaccc ccataagcgc catgcccctc ccgcttctcc
       61 ttgatggagc cgtggagcag ctcgatttcc gcccccaggg ataaatcaac cacctggacg
      121 aaatcggggt tgccaccttg gtaatccttg cggtagagct gggcaccata aacggcggtg
      181 accttatcaa aacctgtggg gatagccagt ccggcagaat aggtttgaga tgtaagcgaa
      241 gcccccaccc cgacggatgc taaagtcgcc gcgccgagca ggataatcga gaagacaagc
      301 aagagtacgc gtttcattga tggttgcaaa cattgggtgc aaatgatgat gcgtgggatg
      361 taaaacattg caccggcaaa atcattttag caaatatttt caataaattg tagcacatat
      421 gttctgttct gagaagatga aaaaatcgac tcttcgtgaa tcttcgtaga agagtggact
      481 tatgggaggt gtgggtattt atgtgggcta atattcggct gagcaatcct gttgtcgatg
      541 atgtcaacga aggttgaatc tatcataaga gcgcgatggg ataaccacat gccatgcagt
      601 acagtgggcg ctatgatggt tcccaaaaaa cattgcaata cccgtataat ggatttctga
      661 tagagagtaa tacctaaggt gaactttccc gctggtaaat ctggatgacc ggatttacaa
      721 atgtagcccg cgttactggt aggaagggtg gtagttggta atcgaacttt ttaatcagct
      781 tgtagttcgt ttcgcctaag aacaatcggc tgtagaattc acactccatc tgccggtaaa
      841 cacaatcgct ctgattgaga aagtccccat acgtgaagct gtccaccagc agataatctg
      901 gctggcggcg ctccacgccg gcctcgccct ggttatactt ggctgaattt ccccccggag
      961 cgggctggtc ggcgtacaac ttatcgaatg atgggtgctc gtaaagcttc gaaaaatgtc
     1021 ccctggggat gttcggcggc gaataggtat attccaatgt cgacccctct ggtagagtcc
     1081 ccagaaacct gctggctagg atgcggttat cattcaggaa taagagcaat accgcgacaa
     1141 cccgcaaaaa ggagtacgca atcacaattg caaggcccac tatactcagc tctcttagat
     1201 accgaatctg gccacgttcg atcctatgta aagcctcttc tacgaaggac cctgccagca
     1261 cagcaagtat aggtatggtt gatagaaggt agcgaggagg gtaacggtac gaaatcaaga
     1321 ctggtagatc tagggcaaag aggcacagta atacgatcgc tatcaactcc attcgcaagt
     1381 catttattaa atgacggccg gcataatacc ggatcacttt caggacatgc caaattagtg
     1441 ccactaggaa gacaagggaa accgcggtac ccagtgaaac cctcagcact gcccattgcc
     1501 cgaagatacc agccacggta tcgggttcta tttcgtagat ggctagccgg cgtatggcag
     1561 gtacggcgtt ttgaaaataa aattccatcg ataatatcga cctcggtgta ccgatggcga
     1621 atccgaaagc acttagcaag gtgcttacga acaacatctc aaaaaaggtg aagggatttt
     1681 cgaagagccc tctccgattg gtgaccagga agatgaacaa ggcggccagg aacaacccca
     1741 ttccgttata tttactggag atcgcaagac ccgcgctaaa gaaggcgatg tatagccata
     1801 ggcgatggcc cttcacacgg tacttaagca gccagaggga tgttaggcag gcgaaaaaag
     1861 ccaggtatat gtctccgtgg gcaaagcggg aataaatggc tgtatcccgg tgagagagca
     1921 caaagagtgc agccagcgtt ccagctagga cgcctcctcc ggcctgccga gtaagaacgt
     1981 aaacgagagc gaccaccaag ccacctagga atacggagac cagccgggca gcctcaatga
     2041 acttcgtctt atccaagccc atagcgtaga ccagtttgcc caccacgtac atgacatatt
     2101 tcggcaggga ggggtagtag aaatcctttt catcgaaccg gtaccctctt tcaatggctt
     2161 tatcagcctg ttgtatcact tcatctggat gccagaaccc aggcgccccc cacgagatgc
     2221 cttgaacagt cagcagcaca aaaattagaa agaaaacaac cggtattact ttatcgtatt
     2281 tgctcacggt gctatactcc caagtaagcc tttgcattgt aatgggtttt tctctgttca
     2341 gcaattctat agagataaga cataaacatt aactaaaggg agaggcactc ctgtccttga
     2401 ggttaccaaa cgaatcgctt gaacagaggc ggaaagaatt tccccgggtg acctaggttc
     2461 cgctttaaag tttctcaagg caatgccaat ttctacttgg aagaagagga taatgctgag
     2521 atatgtctcc gcaataagct agggccaaag gatttacttt gcctttagcc agttccaaaa
     2581 ttctggtttc tgcgtgcctc tcgaaaggcg tgcagaaacc agagtatcga aaatatcttc
     2641 aatcgctcgt aatgatgagt tctgaaggat aaattatatc cccagcaatg ccccaccgat
     2701 gccgagcatc gaatagatta tagcgtaccc gatcatcaga actacgaatc ctatcagagc
     2761 agtgaggaat gccttggtgt tatccaggtc taagccttgg cggatggcga tgaaaccagc
     2821 gatcaacgcc caaatggctc ccaccagacc tccgagacaa gggataatcc ctaggagttg
     2881 cggagcttgg gcgaagccga ggacgcgtag catctcacca agtgtggcct gtccaccgaa
     2941 caggcttgtc cctaccaggt aggacacgcc tgaccaaagg aaccagccga cgaaggccca
     3001 gacgaacgtg cctatgaaac tcgttagaaa attacttccg ctgaagctgg cacccaagcc
     3061 gctgcccaag gcgctcaaca atgcaactag gccgacaact atagctgcct ggctggtggc
     3121 agtctcgtcg tgctcgacct cctcaaatgt attcacgtcg agtttaaaca cacccatgat
     3181 acggttgaac attgcaaact cctccttgtt tttgatgttt tgctatcaca gaattaccca
     3241 tcttttgatc aaattgtatt gatgggtgcc ttcagataat tctattctat ataacccatt
     3301 tgcctagcag aggttgctgg cagatgagac aagaactagc caatattcca ggtattactc
     3361 tagccgtttg ggtgatgtgc gcgggcttcg tgttggctat acttatgaag ctaggcagtt
     3421 ggattttttc ttcgaatgcg taagtcctaa ctacacaatg tattgttatc taaatggagg
     3481 aagtctatgt cgaacaaatc gaacccattg accgctcttt ccgatgcgat gaccgaagcg
     3541 gtcgccaagg ccggggcggc aaccgtgcta gtaaacgccc ggcggcgttt cccagccagc
     3601 ggcatcgcct ttacacccga cttaatccta accgccgatc atgtagttga gcgcgatgag
     3661 gatatcctcg tcgtgctgcc ggatggcagc caggtaaaag ctaccattgc cgggcgcgac
     3721 tccgggagcg acctggcctt gctgcgccta gagcgtgctg cggcctcgag cgcagagcca
     3781 gcctcgcaag acccccgcgt ggggcagata gcgctctcac tgggtcggcc gacgcaagaa
     3841 ggcatccagg ctagcctggg aatcgtcagc gccattggtg gtccggtgcg taccgggcgc
     3901 ggcggcctgc tggaccgcta cctgcgcacc gatgccatcc cctaccccgg cttctctggc
     3961 gggcctttgg tggataccgc cgggcgtgtg atcggtgtga acacatcggg gctgacgcgc
     4021 ggcgcctcat tagccatccc gattaacctg gcctggcaga cggctgagac gcttgccagg
     4081 catgggcgtg tacgccgcgg ttttttgggc gtgcgcagcc agccggtctc tattcccacc
     4141 tctcagcaga aagcgctggg ccgggaccag agcagtggtc tgcttttggt aggcgttgag
     4201 gagaagagcc ccgccgatgt tggcggcctt ttggtaggcg atatcattgt agccattgcc
     4261 gggcaatcgg taaccgacgc cgaccagctg ttatcctccc tcgcgggaga tgtggttggc
     4321 aagccaaccg cggtagatgt attgcgcggc gggcagcctg tgaccattac ggttacaatt
     4381 ggggagcgta aggactagtt atggacgtgc aaacgctctc caatcagatc gcagacgccc
     4441 tggctgaggt agcgctgcgc gtgcagcgca gcttggtggt ggtgcataac gggcgccacg
     4501 gtgctggtgc tggggtggtt tggcgacagg ggggctacat catcactaac catcatgtca
     4561 tcgcccgcgg taggatcgct gtaaccttac cggatagagg cgagttgccg gcgcgagtgg
     4621 tagcgaagga gccgcagctg gacctggcga tccttcaggt gcaaaatgac agcttggctc
     4681 cggcacggat tgccgattcg cgtgttttgc aggtaggcca gattgtgctg gcggtgggcc
     4741 atccctgggg tcagcggggt gtggtaaccg ctgggatcat cagcggttta ggaactgctc
     4801 aaacgcgcga tcagcgccaa tcagtgccta ttatccgctc cgatg
//
LOCUS       DBNGSX010000318        10072 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1272918, whole genome shotgun sequence.
ACCESSION   DBNGSX010000318 DBNGSX010000000
VERSION     DBNGSX010000318.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10072)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10072)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10072
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1272918"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..377
                     /locus_tag="AC16CB_09535"
     CDS             138..377
                     /locus_tag="AC16CB_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342750.1"
                     /translation="MKNSFQTWAKCINEDLDAFAAYVRGRKQDFKLASPIAQPPPDSL
                     YGADVYIGGAILFHALRLQVGEVAFFEALRTYAER"
     gene            complement(529..705)
                     /locus_tag="AC16CB_09540"
     CDS             complement(529..705)
                     /locus_tag="AC16CB_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342751.1"
                     /translation="MNRTKILSFILAFVIVGINLLAIYRIGIAYNSYSWKKLAAYYPP
                     QPEVYMLYLPNVQR"
     gene            complement(740..2197)
                     /locus_tag="AC16CB_09545"
     CDS             complement(740..2197)
                     /locus_tag="AC16CB_09545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 117 protein"
                     /protein_id="HIR3342752.1"
                     /translation="MRKIHRSSSLPDWLWGVIVVTLPLIVYLHTMAPTVYGLDSAELT
                     TGAYTLGIVHPPGSPTFLLLGYLFTWLPIGDVGYRVNLLSTFAAALVVLNTYILLKVL
                     TDKRIIALGTAWLFAFTYYPWVSALAAELYALQALFTVVTILLAILWRESGNPRYLYG
                     LGLLLGLGLGNHLSIVLLIPGLAWIVLSRSGTWRRAGWLSAAVGCFLLGTAIYLYIPI
                     RYASGTSLNYARQYWGVELNTFEGFFWMISARMFKSLFWAVPTREIPAHIYHFIQQLW
                     SNFLGMGFLLGLIGIFVVFRQRFWLFFGLLLMWVNYLAFYIPYGVGDKEVMFLPVYLI
                     WAIWVGVGANRLDAAIQKHKSNKYRDLLTVGMLVFAGSSLAFNYAYVDLSQDYSARKI
                     GEKIFQSIEPKAMYFGTWYDVPILEYLQWVENQRKDVTTVNLLFISTASAQNLARKHL
                     LAHQPVYTSTIDRFPKSEFTSYRVKQCECYRVEIP"
     gene            complement(2246..4006)
                     /locus_tag="AC16CB_09550"
     CDS             complement(2246..4006)
                     /locus_tag="AC16CB_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342753.1"
                     /translation="MLPKDIFWSPDEGARFIIAQILNPNSDKPNHLGYPGAKMDPVYK
                     FHPSIYQKGMYPTPKADGTIKYAWSIWFPMITGWLYTKRGFSGLYLIPLVSGLLIVIM
                     TWHLSNFLNNPYPFWTVILVGLGTPIYFYSVVYWDHTLASLLGILALFGLVRYRRPVI
                     QWLWLVLLPLIIAVILRVEMLSFTLSLALTFGIANSLKVSTSQLPREKIHIGKTQINL
                     NHTFSQNFRIFRTPLLVAMAIGFFLVFFKFLLPNLPERHYLDIRQFFGALTNSTNPWK
                     VGSSLILRMSKHLPHLLINYSAAEGLHYPEYVNLVGCIALILCLVGAFIRSTFLKYAL
                     PIPGFVILVSLGLYTIFSSQPYRSLHGLFISAPFGAVFVYPLVEGWRKRNYSDVMVSL
                     CASMYFVIGSGVILGIRSSRVETRWPGLEWGSRYLLTIYPIFVLLSLKFLNNMRKAHP
                     LGLIKYLITGLIGLMMLSGILLQVRGIWMLKQSKQQISSWQGYLQNQLPDAVVSDIWW
                     IPASLAKYFTEHEMYAIRDSQDMSNWLSLAIEYGIDRFTYVSFLPKNLENYSLANVKI
                     LRKNSKDIHGIIFVNYTIER"
     gene            complement(4161..4657)
                     /locus_tag="AC16CB_09555"
                     /pseudo
     CDS             complement(4161..4657)
                     /locus_tag="AC16CB_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015045058.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4942..5130)
                     /locus_tag="AC16CB_09560"
     CDS             complement(4942..5130)
                     /locus_tag="AC16CB_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342754.1"
                     /translation="MTLSTPKTQTAQVAMAMTQGSTISPYNLQMAASLLTVAVPLMIF
                     LFLGRYFIRGILAGVLKG"
     gene            5291..5656
                     /locus_tag="AC16CB_09565"
     CDS             5291..5656
                     /locus_tag="AC16CB_09565"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HIR3342755.1"
                     /translation="MKYLSPAQQADFRNKVWDIVRQVPGGRVITYGNIAALIPPPKGM
                     DPKDYAAWSARWVGGAMAACPEDVPWQRVINSQGKISLRPSSSALQQRQLLEAEGVVF
                     DSRDRVDLKIFGWAGSPEE"
     gene            5697..6596
                     /locus_tag="AC16CB_09570"
     CDS             5697..6596
                     /locus_tag="AC16CB_09570"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3342756.1"
                     /translation="MTTPFRIDPRIRAYGKARIDVTRRARDEREWQTQWREPRYPFPF
                     RWEGGWRHCIQYTVDDFAAEIGFYIDVLGFPVNAFSPSYAQFTSPDQAFFFAVAAIQE
                     GAQSTPFDSLRMQFMVEDLPATIEELEKRNISFERKPESDEVFPAIAILRSPHGIGIE
                     LWGVVRDQPRDATHEMSEEDDTADFWADDDEVEDEVDEQEADDTQDDAEQPSYEGIGD
                     GELVDEPVYVDEDEAGQKEPLHPIAANPTDGSSRSIPSLPRAVIRRTEDLLKGGVQFP
                     VRKVRGNGFRNQKLLDVNGDPGE"
     gene            6725..7291
                     /locus_tag="AC16CB_09575"
     CDS             6725..7291
                     /locus_tag="AC16CB_09575"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3342757.1"
                     /translation="MKLDFHPVTPDRWSDLETLFGPRGATGGCWCMYWRLKRSEFEQM
                     KGEGNRQAMKSIVDSGEIPGLLAYHDGQPVGWISVAPRPAFSALERSRILKPVDERPV
                     WSVVCFFVKKSFRRKGVTVELLQAAVEYARQHGAQIVEGYPVEPRQGQSPDPFVYTGL
                     ASAFLQAGFEEVLRRSETRPIMRRVIGG"
     gene            7346..7906
                     /locus_tag="AC16CB_09580"
     CDS             7346..7906
                     /locus_tag="AC16CB_09580"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIR3342758.1"
                     /translation="MLNRCSWAGSDPLYVAYHDQEWGVPVHDDHLLFEFLILEGMQAG
                     LSWAIILKKRDNFRRAFDGFDPQRVAQYGDEKVAALLADPGIVRNRLKISAAIRNARS
                     FLEIQSECGSFDAYIWQFVGGKPKVNAWRNLTEIPASTPESEAMSKDLARRGFKFVGS
                     TICYAFMQAVGLVNDHTVDCYRYNEI"
     gene            8130..9131
                     /locus_tag="AC16CB_09585"
     CDS             8130..9131
                     /locus_tag="AC16CB_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3342759.1"
                     /translation="MDFAIETEHLSRIYKLRGSKKSEPKQLVALEGVNLQVRRGELFG
                     LLGPNGAGKTTLIKILTTLLSPSSGWARVAGFDVVKEPQNVRLHINMVSGGESSGYGL
                     LTVRENLWMFSQFYGMPSAQANQRIKELLQVVGLSDRINTKSSDLSTGLRQKMNIVRG
                     FLTDPDVLFLDEPTLGLDVGASRDVRRFIRRWVDENPSRTILITTHYMAEADELCDRV
                     AIINKGQVLACDTPATLKHRLQGEAIIRMEVSPLNGLGVDIFESLPGVRKVVHRPQDG
                     FSVLEFILEQDDALGAVVNTMTSANIHILNLHKREPSLEDVFVDLVGRSMEQVEKGE"
     gene            9128..9958
                     /locus_tag="AC16CB_09590"
     CDS             9128..9958
                     /locus_tag="AC16CB_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3342760.1"
                     /translation="MRVKKNSGWILFMQAVIARAYPRVIGQQREKSWMFFEIFLPMLS
                     VAAYVFVYRAIGAPEEYVGFVVLGGAMMAFWLNVLWNMSSQLYWEKEQGNLALYIIAP
                     NSMMAILLGMAVGGLFATLLRAVVIVGLGSWMFQVRYAIASFPLLCAVFILSMVALYG
                     LGMMSASIFLLLSREAWHLVSLAQEPVYLASGFYFPIKNLNFWVAVAASFIPLTLGLD
                     AMRQLAFPSGESLGFLSVRVELGVLLVLCFLFLLGARWLLNYIEKLAIREGRLTESRR
                     "
BASE COUNT         2647 a         2300 c         2534 g         2591 t
ORIGIN      
        1 aggaaaatca tcaggggcac ggcgacggtt aaaagagaag cagccatttg caggttgtat
       61 ggtgaaatcg tgctcccttg ggtcatcgcc atggcaactt gggcagtctg cgtttttgga
      121 gtgctcagcg tcaaaggatg aagaactcct tccagacttg ggcaaaatgt attaacgaag
      181 atctggatgc ttttgccgca tatgtccgcg ggcgtaagca ggacttcaag ctggcctcac
      241 ctattgcaca gccgccacct gattcgctgt acggtgcgga tgtctatatt gggggggcga
      301 tcttatttca tgccttgcgg ctgcaggttg gtgaggtggc cttcttcgaa gctttgcgta
      361 cctatgccga gcgctaacgg gacagcaata ccagcgtgaa cgagttcatg caggtagtag
      421 aggcgagcag cgggcaagaa ctggcgcaat ttttcgatgc ctggctcatc gtgaccgaac
      481 tgcctcccta ccaggagaga aataatttac attaattagc tttatttttt atcgctggac
      541 atttggtaaa taaagcatat atacttctgg ctgaggaggg taataagcag cgagtttttt
      601 ccagctatat gagttataag caatcccgat tctataaatt gctagcaggt ttattcctac
      661 gatgacaaag gcaaggatga aagataatat ttttgttctg ttcatttctt aatctcctca
      721 caaaagtagc tgacttaatt tatggtattt caacacgata gcattcacat tgtttcaccc
      781 ggtagctggt aaattcagat ttgggaaatc tgtctattgt ggaggtataa accggttggt
      841 gagccaaaag gtgtttgcgt gccaaatttt gagcagatgc tgttgatata aacaatagat
      901 ttactgtggt cacatccttt ctctggttct ccacccattg gagatattcc aggattggta
      961 cgtcgtacca ggttccaaaa tacatggcct tgggttcaat ggattgaaat atcttctctc
     1021 caattttacg tgcgctataa tcttggctca aatcaacata ggcataatta aatgctagac
     1081 tgcttccagc aaaaacaagc ataccaactg tcaataggtc acgatactta tttgatttat
     1141 gcttctgtat tgctgcatcc agcctgtttg cgcctacccc tacccaaatt gcccaaatta
     1201 aatagactgg taggaacata acctccttgt ctcccacacc ataagggata taaaatgcca
     1261 ggtaatttac ccacatcaag agtaacccaa agaataacca aaagcgttgt ctgaaaacga
     1321 cgaaaatacc aattaaacca agcaagaaac ccatacccag aaagttgctc cataattgtt
     1381 gtatgaaatg atagatgtgt gctggtattt ctcttgtggg aacagcccaa aatagcgatt
     1441 taaacatgcg agcagagatc atccagaaga aaccttcaaa ggtatttaat tcgactcccc
     1501 agtattgcct ggcgtagttt agagaagtac ctgaggcgta gcgtatgggg atgtaaaggt
     1561 atatagcagt acccagtaaa aagcaaccta cagccgcaga gagccatccg gctctacgcc
     1621 atgtgcctga tctgcttaaa acaatccatg ctagacctgg aatcagtaaa actatggaaa
     1681 gatggtttcc cagacctaac cctaagagaa gccctaagcc atacaggtat cttggattac
     1741 cactctcacg ccacaatata gctagcaaga tcgtaactac ggtaaaaagt gcctgcaaag
     1801 cgtataattc agccgccagt gcgctcaccc aaggatagta ggtaaaggca aataaccagg
     1861 ctgtaccgag agcgatgata cgcttatccg ttaatacctt gagcaggata tatgtgttca
     1921 gaaccaccaa ggcggcagca aaagtggata gtaagtttac acgatagcca acatctccaa
     1981 ttggcaacca ggtaaacagg taaccaagca acaaaaaagt tggtgagcca ggagggtgaa
     2041 caattccaag cgtgtacgca cctgtggtga gttctgcact atccaggccg taaacagtgg
     2101 gtgccatggt gtgtaggtaa acgatcagtg gcagcgtaac tacgattaca ccccataacc
     2161 agtctggcaa cgatgatgaa cgatgaattt tacgcaactg atgaatccgg tacacgccaa
     2221 tttctaaaca agtgtttcgt ggtccttaac gctctatcgt ataattcacg aaaattatgc
     2281 cgtggatatc ttttgaattt ttcctcagga tttttacatt tgctaggctg taattctcca
     2341 aattttttgg taaaaagctc acatatgtaa atctatcgat gccgtattca atggcaagcg
     2401 ataaccaatt ggacatatcc tggctatctc gaatagcgta catctcatgt tcagtgaaat
     2461 attttgctag ggatgcaggt atccaccaga tatccgagac cactgcatct ggtagttggt
     2521 tttgcaaata tccctgccag gaagaaattt gctgtttgct ttgctttaac atccatatcc
     2581 cgcgaacctg taaaagaatg ccgctaagca tcatcaaacc aatcaaacca gtgattagat
     2641 atttaattaa gccgagtgga tgtgcttttc gcatgttatt taggaatttc aaagacagaa
     2701 gcacaaaaat gggatagatt gttagtagat aacgtgaacc ccattccaga cctggccagc
     2761 gtgtctctac ccggctgctg cgtataccca agataacccc cgaaccaatc acaaagtaca
     2821 ttgatgcgca taggctaacc attacatctg agtaattgcg ttttctccag ccttcaacca
     2881 gaggataaac gaaaactgct ccaaaaggcg cactgataaa gaggccgtga agagaacggt
     2941 agggctgcga tgagaaaatg gtataaagac ccagggatac cagaatcacg aagccaggta
     3001 tcggtagggc atatttcaga aaagtagatc gaataaacgc tccaacaaga cataagatca
     3061 gggcaatgca accaaccagg ttgacatatt ctggatagtg taatccctct gcggcactgt
     3121 aattgatcaa aagatggggt aggtgcttgc tcatgcggag aatcaagctg ctcccaacct
     3181 tccaggggtt tgtcgaattg gtcaacgcgc caaagaattg gcggatatcc aggtaatggc
     3241 gttctgggag gttgggaaga agaaatttga agaataccag aaaaaaacca atcgccatag
     3301 ctaccaaaag aggcgttcgg aatatcctga aattttgtga aaaagtatga tttaggttta
     3361 tttgggtctt accaatgtgt atcttctctc tgggaagttg agatgtactc accttcaggg
     3421 agtttgctat accaaatgta agagccaaag aaagggtaaa agacaacatc tccacccgca
     3481 aaataactgc aattatgaga ggcaataaaa ccagccacaa ccattgaatt acaggtcgtc
     3541 tgtaccgtac caatccaaat agagctagga tgcccaacaa cgacgccaac gtgtgatccc
     3601 agtaaacaac actatagaaa taaataggtg tacccagccc aaccaaaatg accgtccaaa
     3661 atgggtacgg attattcagg aaatttgata ggtgccaggt cattatcacg atgagtaaac
     3721 cgctcacaag aggtattagg tataaacctg aaaaacccct tttcgtgtat agccatccag
     3781 ttatcattgg gaaccagatt gaccaggcat acttgatagt accatctgct tttggagtgg
     3841 gatacatacc tttctggtat atgcttggat gaaacttgta aaccggatcc atttttgcgc
     3901 ctggatagcc cagatgattc ggtttatctg aatttgggtt gagaatttga gcgataatga
     3961 agcgtgcgcc ttcgtctggt gaccaaaaaa tatccttagg taacatcacg agcgttgcca
     4021 ggtacgtgca taaaacaatc gtaaaagcaa taattatctt tatccgggat tccacgtcta
     4081 tatcatttta tcaatctatt cgcccaaacc gagcgccatt caacaaactc gcatacatga
     4141 aatgcttttt gctggtcagc ttaaatataa ataatggaat ggtgcataca aaaattcttt
     4201 tcaagacaga ctttttttca tagatctctc gatatggata tttttccgag agcttcatta
     4261 ccacacttct tgctgttctg acgatcttcc ccgccagaaa aatgtacttc aagcgaaacg
     4321 tcttgatttg ctgccagtat tccgttgtct ccaagaagtc aatctttaac agcaagaaga
     4381 gattgtaagc caccatcatc aactgaaaga tcgcttcatt tgaccaaaat gattggagta
     4441 acaaatggcc aaccgccgtt tcatacttgg cctccttgat gtaattctca cagttgccgc
     4501 gtttttgata aaaatcgacc acttcctctg aagacagctc tgtgttggtg acatagaaga
     4561 agtattggta ttcttcacct gccaggaagg ataattgcgc gctttctttt ttctctttca
     4621 atacccgcgc gacgataatt cttctgtctt ttttccagag gaagacaaat acatatgaac
     4681 attcagtcga taacagtatg aagcttgctg aatcgcagac atcacctcct ttcacctcac
     4741 ccagaagatg accgataaac atcccaccca agtactagat aaggtcgcgc agcttaatat
     4801 atttcgccag cgatggaaca aagatgtatg gtctccccca atattcgtag aactaagcct
     4861 gtgacctttt taaattatac agaaagatat atgcgatgta aagcatttcc ggttgaaatg
     4921 ggaaatgcga aaaaaacaat gtcaaccctt tagcacccca gccaggatgc cgcgtatgaa
     4981 gtaacgaccc aggaacagga aaatcatcag gggcacggcg acggttaaaa gagaagcagc
     5041 catttgcagg ttgtatggtg aaatcgtgct cccttgggtc atcgccatgg caacttgggc
     5101 ggtctgcgtt tttggagtgc tcagcgtcaa aaatatgaaa tgatcttgac atagaacaaa
     5161 cgttcgtgta tatttaactg cagagactga tacagatctt ttgtcgttct attcgatata
     5221 aataatcgat gcaatcagga ttagttttga gacaattcac attaagtgag atcacaagga
     5281 gacctgaaga atgaaatacc tgtctccagc acagcaagct gattttcgta ataaagtgtg
     5341 ggatatcgtt cgccaggtcc ctgggggtag agtaatcacc tatgggaata tcgccgcgct
     5401 tatccctcct ccgaagggta tggatccaaa ggattacgcc gcctggtctg cccgctgggt
     5461 gggtggagct atggcagctt gcccagaaga tgtcccctgg caacgggtga tcaattctca
     5521 aggcaaaatc agcctgcgcc cctcaagcag tgccttgcag cagcgccagt tattggaagc
     5581 tgaaggtgtt gtattcgatt cacgcgaccg tgttgatttg aagatatttg ggtgggctgg
     5641 gtcacccgaa gaataacttc aagcctattg gttggtattt ccaggagatg gatgacatga
     5701 caacaccatt tcgaatagat cctcgtatta gagcctatgg caaggcgcgc atcgatgtca
     5761 ctcggcgtgc gcgcgatgag cgtgagtggc agacgcagtg gcgagagcct cgctacccat
     5821 tcccgtttag atgggaaggc ggttggaggc attgcatcca atatactgtt gacgatttcg
     5881 ccgctgaaat cggtttctac attgacgtac taggatttcc agtcaatgcc ttctccccct
     5941 cctatgccca atttaccagc ccggatcaag cttttttctt tgctgtggcc gctatccagg
     6001 aaggtgctca gagcacgccg ttcgattcgc tccgcatgca gttcatggtg gaagacttgc
     6061 ccgctacgat cgaggagttg gagaagcgaa acatcagctt tgagcgcaaa ccggaatcgg
     6121 atgaagtttt tcctgccatt gccattttgc gttctccaca cggcatcggg atcgaattat
     6181 ggggtgtggt tcgtgatcag ccacgtgatg ccacccatga gatgagcgaa gaggacgata
     6241 ctgccgattt ttgggcggat gatgatgagg tggaagacga agtagatgaa caggaagcag
     6301 atgataccca ggatgatgcg gaacagccat cctatgaggg tattggcgat ggtgaattag
     6361 tagatgagcc agtgtatgtg gatgaggatg aggcaggaca aaaggagcct cttcacccaa
     6421 tagctgctaa tccgacagat ggatcatctc gttccatccc atccttgcct cgggcagtca
     6481 ttcgccgtac ggaagacctg ctcaaaggcg gcgttcaatt tccagttcga aaggtgcgtg
     6541 ggaatggctt ccgtaatcaa aagctacttg acgtgaatgg cgacccagga gagtagtagc
     6601 atcgtcaagt aatttcaaaa tgatatatat ccagacagcc gctcgggaga accgggcggc
     6661 tgatggaatc ccagcacttc agcattagag gtagaatcaa gtttcgatag gaggctcccc
     6721 acccatgaaa ttagactttc atcccgtcac ccccgaccgc tggtctgacc tggagaccct
     6781 gttcgggcca aggggtgcca ctggaggttg ctggtgcatg tactggcgct tgaagcgctc
     6841 tgaattcgag caaatgaagg gcgagggaaa ccgccaagcc atgaagtcca tagtggactc
     6901 gggcgaaata cccggtctgc tagcctacca tgatggtcag ccggtgggct ggatctctgt
     6961 tgcgccgcgc cctgcatttt ccgccctgga acgctcgcgc atcctcaagc cggtagatga
     7021 aaggccagtc tggtcggttg tatgtttctt tgtgaaaaaa tccttccgcc gcaaaggcgt
     7081 gacggtggaa ttgctgcagg cagctgttga atacgcccgc cagcatggag cgcaaattgt
     7141 tgagggctat cctgtggagc ctcgacaggg acagtcgcca gacccctttg tctatacggg
     7201 tttagcctcc gccttccttc aggcgggttt tgaggaagtg ttacgccgtt cggaaacccg
     7261 tccgattatg cgcagggtaa ttggtggtta atctgctcga ttattaaacg ggtacagctc
     7321 tattccttat ttggagattt gtattatgct aaatcgttgc tcttgggcag ggtcggaccc
     7381 gctctacgtt gcctaccatg atcaagagtg gggcgtgcca gtgcatgacg atcatctgct
     7441 cttcgagttc ctcattcttg agggcatgca agccggctta agctgggcta tcatactaaa
     7501 aaagcgtgat aacttccgac gcgcctttga tggctttgac ccccagcggg tggcgcagta
     7561 tggcgatgag aaggtcgctg ccttgcttgc agaccccggc attgtgcgca accggctgaa
     7621 gatctctgca gcaatccgga acgcgcgctc tttcctggag attcaaagtg agtgtggcag
     7681 tttcgatgcc tatatctggc agttcgttgg aggaaaaccc aaggtcaatg cctggcgaaa
     7741 cctgacagag attcccgcca gtacgcctga gtcagaagca atgagtaaag acctggcgcg
     7801 tcgcggcttc aagttcgtcg gttcgaccat ctgctatgct ttcatgcaag ccgtagggtt
     7861 ggtcaacgat cataccgtgg attgctatcg atataatgag atttgaattg aagagaaact
     7921 atttgttgcc aaaaaggggg attgagaggg ggcctgtgtt agaatcggcg agagccgggt
     7981 acgccgcccc cggctctctc taaggaggag aagaagagaa atcgcccttc aattgttata
     8041 ttaccagagg cgacgaatct ctgcttgacg attaccctac gattacccta cgattgccca
     8101 tcaattttga gggttttggc aatattacta tggattttgc aattgaaacc gaacatttga
     8161 gcaggattta caagttgcgg gggagtaaaa agagcgagcc caagcaactg gtcgccttgg
     8221 agggcgtcaa tctgcaggtg cgccgtggcg agttgtttgg cctactcggt ccaaatggcg
     8281 caggaaagac caccctgatc aagatcctca ccacgctgct ctcgccctcc tctggctggg
     8341 cacgcgtagc gggtttcgat gtggtcaaag aaccccaaaa tgtacgccta cacatcaata
     8401 tggtctctgg gggcgagtcg tctggttatg gcctgctcac ggtgcgggag aatctgtgga
     8461 tgttttccca gttctacggc atgccctcag cgcaggcgaa ccaacggata aaagagctac
     8521 tgcaggtggt ggggttgtcc gatcggataa ataccaagtc atctgatctc tccaccggct
     8581 tgcggcagaa gatgaacatc gtgcgcggtt ttctcaccga cccggacgtg ctcttcctag
     8641 acgagcccac tctggggtta gatgttggcg cttcgcggga tgtacgccgc tttatccgca
     8701 ggtgggtgga cgaaaacccc tcccgtacaa ttttgatcac tactcactac atggcggagg
     8761 ccgacgagct gtgtgaccgg gtggcgatca tcaacaaggg gcaagttctg gcctgcgata
     8821 cacccgcaac cttgaagcac cgtttgcaag gcgaggcgat tattcgcatg gaggtcagcc
     8881 cgctcaatgg attgggagtg gatatcttcg aatctctacc aggagtacgc aaggttgtcc
     8941 accggcccca ggatggtttt tcggtcttgg agttcatcct cgaacaggat gatgccttgg
     9001 gtgcagtggt caacaccatg acgtctgcta atattcatat tctcaacctg cataagcgcg
     9061 aaccttcact agaagatgtc tttgtcgatc tggttgggcg tagtatggag caggtggaga
     9121 aaggcgaatg agggtcaaga aaaacagtgg ctggattttg tttatgcaag ctgtgatcgc
     9181 gcgcgcctac ccgcgagtga ttgggcaaca gcgggagaag tcctggatgt tctttgagat
     9241 attcttgcct atgctctctg tggcggctta tgtgtttgtc taccgcgcca taggcgcgcc
     9301 ggaggaatac gttggcttcg tcgtcttggg tggggcgatg atggctttct ggctgaacgt
     9361 cttatggaat atgtccagcc agttgtattg ggagaaggag cagggtaacc tggcgctgta
     9421 catcattgcg cccaactcga tgatggccat cctgctggga atggcggtgg gcgggctgtt
     9481 tgccaccctg ctgcgtgcgg tggtgattgt ggggttgggg agctggatgt tccaggttcg
     9541 ctatgcgatc gctagctttc cgcttttatg cgccgtgttt attttatcaa tggtagccct
     9601 gtatggtctg gggatgatga gtgcctcgat ctttctgtta ttgagccggg aggcatggca
     9661 tctggtaagt ttggcgcagg agccggtcta tttagcttcg gggttttatt tccccatcaa
     9721 aaatctcaat ttttgggtgg ctgtggctgc ttccttcatc cccctcaccc tggggttgga
     9781 tgccatgcgg cagcttgcct tcccctccgg tgaaagcttg ggtttcctct ctgtgcgggt
     9841 tgagctgggc gtgctgctgg tattatgctt cctttttcta ctgggtgcac gctggctgtt
     9901 aaattatatc gaaaagcttg ccattcgcga ggggcggctg acagagagcc gacggtaaat
     9961 gatgatgtgg cgctcgtttc gcatggcgtc ctggttgggc tggcagatcg aatcgaactg
    10021 ggcagatccg ttcctgttcg cagtatattc gttcgccaaa cccttggcag gg
//
LOCUS       DBNGSX010000319         3860 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1133863, whole genome shotgun sequence.
ACCESSION   DBNGSX010000319 DBNGSX010000000
VERSION     DBNGSX010000319.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1133863"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="AC16CB_09595"
     CDS             <1..598
                     /locus_tag="AC16CB_09595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR3342761.1"
                     /translation="LERVQAVIQSGARHLGENYAEEGVEKLEALSGPLGVTWHMIGHV
                     QSRKARLVSNHYDWVHSLDSLRLAEKLNRFAAESGRRLSILLECNVSGEESKYGFPAW
                     VDERWEDLAEAVTPILNFSNLDMRGLMTMAPYAVEPEAARPYFRRLRLLRDFLSLKFP
                     NADWSQLSMGMSGDFETAVQEGATIVRVGEAILGLRPG"
     gene            612..869
                     /locus_tag="AC16CB_09600"
     CDS             612..869
                     /locus_tag="AC16CB_09600"
                     /inference="COORDINATES: protein motif:HMM:NF014389.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIR3342762.1"
                     /translation="MQFIIYFINLLVQVLSLLVIAHVILSYFMSPYHPVRQVIDSVVE
                     PLLSPIRRIMPQTGMLDFSPMVLIILLQVVARLLVGLLISF"
     gene            880..1191
                     /locus_tag="AC16CB_09605"
     CDS             880..1191
                     /locus_tag="AC16CB_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HIR3342763.1"
                     /translation="MKRRQYHLHNGQKGAALAIRVTPRASKNEIAEIRSDGTVRIRLM
                     APPIEGRANDALVTFLADILDVSRSKIEIVAGETGRDKLVSVIGMDAETVHKKILQHL
                     G"
     gene            complement(1234..3756)
                     /locus_tag="AC16CB_09610"
     CDS             complement(1234..3756)
                     /locus_tag="AC16CB_09610"
                     /inference="COORDINATES: protein motif:HMM:NF017539.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR3342764.1"
                     /translation="MIRFLRSFELDRLSFWLGFIAGILLIWLLGRLRPWVARLFQSLR
                     ERSQLSKLGAATVTEIRLRNDLLRHSQGLHLASPLFSLDEISLTPRLLAPPALTEPGI
                     TPPSIDIAENILPPLPDAPELASFYGAPSFTALEALGGNAHLLIIGHSGSGKTVTLAK
                     LCADIIQADQLPSNLKDSLPILLHAADITLPSSNPDDLLEPLVIAVAQICPSVPQARL
                     EKFLPSAFSGGRTLLLLDGLDELPPADVTIYVEYIKNLLDHYPDTRLIATATPDFFDG
                     LTAIGCVPLTLASWNDKQRREFINRWSDMWINHIGSMTKDTEALDPNLLNAWLTNDNS
                     ILSPLELTLKVWATYAGDLPGPHKTDWIEAYLQRVTVDIPKGRPALELLTRHAVNTRK
                     PIFTLRDAQNWLAEIEPSPAPDAAPGVEIEDANWQPIDTERPKSSPQAPIRDSRVLPA
                     LIESGLLISHQNETLSFSHPLILSYLAACGFAKNGGVSSMTKDSQWPVYLQTLGFLAT
                     KSPDADSFVRPYFEKSPEPLMRDIFTAARWLRYAPESASWRAHIMRQLARVMQNESYP
                     MALRARALTALTLSGSSGVDTLFRQFLTSKDNALRQLGALGCGALQDSKSINDLISLL
                     GQNHPNLRQAACLGLVAIGNQTALEAVAESLLNGDDELRQTAAEALANNIEEGHPTLK
                     EGATLEDFLVRRAVVYGLRRVGQPWAIQTLERMQVSDEQWVVKDAAARVLDELAKPKV
                     RLPKSMPDLTETPWLIAFAGERGIGISPGKPALDLLLLALKEGNEEQRLSALDYLARY
                     GEANAIPVILEIYATQRGEVCEAAYNTLWHIAASGVQLPTLS"
     gene            complement(3759..>3860)
                     /locus_tag="AC16CB_09615"
     CDS             complement(3759..>3860)
                     /locus_tag="AC16CB_09615"
                     /inference="COORDINATES: protein motif:HMM:NF014630.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HIR3342765.1"
                     /translation="ACGSSLNAALLQAARSPNQLSRCESCGRILYSG"
BASE COUNT          955 a          869 c         1062 g          974 t
ORIGIN      
        1 gttagaaaga gttcaagcgg taatccaatc tggagcacgg catttaggag agaattacgc
       61 tgaagaaggc gttgagaagc tcgaggcgct ttccggtccg cttggtgtca catggcatat
      121 gatcggtcat gtgcagagcc gcaaggcaag actggttagc aaccattatg attgggtgca
      181 ttcactggac agtttgagac tcgcggagaa gctaaatcgc ttcgcagcgg aatctgggcg
      241 caggttatcc atcttgctcg aatgtaatgt cagtggcgag gagagcaagt atgggtttcc
      301 agcctgggta gatgagagat gggaggattt agctgaagct gtaactccaa ttttaaattt
      361 ctcaaacctt gacatgcgtg gcctgatgac gatggcgccc tatgctgtgg aaccagaggc
      421 cgcacgccct tattttcgcc gcctgcgact attgcgcgat ttcctatcat tgaagttccc
      481 caatgcggat tggtctcaac tatctatggg gatgagtgga gacttcgaaa cggctgtgca
      541 ggaaggggca actatcgtgc gggttggtga agcgattctt ggtctaagac caggttaaaa
      601 ggagaagaat tgtgcagttt attatttatt tcataaattt gttggtccag gtgctctcat
      661 tgttggtgat tgcacatgta attctctcgt attttatgtc cccttatcac ccggtgcgcc
      721 aggttattga cagtgtggtt gaacctcttc tgagtccaat ccgccgcatc atgccgcaaa
      781 ccgggatgct tgatttcagt ccaatggtgt tgattatctt actacaggtt gtagcccgat
      841 tgctggtggg gttattaatc tcattttgag agaaaagatg tgaaaaggcg acaatatcac
      901 ctacataacg gccaaaaagg agctgccctt gcaatccggg tgactcctcg agccagcaag
      961 aacgaaattg ctgaaatccg cagcgatggc acagtgcgca tccgcttgat ggcaccacct
     1021 atcgaaggca gagcaaatga tgctctggta acatttttgg cggatatctt ggatgtctcc
     1081 cgttcgaaga tcgagattgt ggctggggaa acgggacgcg ataagcttgt ttctgtcatt
     1141 gggatggatg ctgagaccgt ccacaagaag atcttgcagc atctgggata aatttaattt
     1201 ttctttctcg tctatcaggt ggcgagtaaa aaactaggag agtgtgggca gttgaacccc
     1261 tgatgccgca atgtgccaaa gcgtgttata ggcggcttcg catacttccc ctctctgggt
     1321 cgcatagatt tccaaaataa ccggaatggc gtttgcctct ccgtaacgtg ccaagtaatc
     1381 gagggcggat aagcgttgtt cctcatttcc ttctttcagt gccagcagga gcaaatctaa
     1441 ggcaggttta cctggtgaga taccaatacc acgctcgcca gcaaatgcaa tcagccaagg
     1501 cgtttctgtt aagtcaggca tgctttttgg taggcgtacc tttggtttgg ctaactcatc
     1561 caatacgcgt gcggctgcat ccttaaccac ccattgttca tcgcttactt gcattctctc
     1621 caatgtttgg attgcccaag gctggcctac ccgtcgcaat ccgtatacca ctgcgcgtcg
     1681 taccagaaaa tcttcaaggg ttgcgccttc tttgagcgtt gggtgacctt cctcaatatt
     1741 attggctaaa gcctcggcgg ctgtttggcg aagttcatca tcgccgttta acaaggattc
     1801 agctactgcc tccagcgctg tctggttacc aattgcgact agccccaagc atgcggcttg
     1861 gcgcaaattg gggtggtttt gtcccaacag gctaattaag tcgttgatgg acttggaatc
     1921 ttgcagagca ccgcaaccca aagcgcctaa ttggcgcaac gcattatcct tagaggtgag
     1981 aaactggcga aatagtgtat ctactccaga gctgcctgag agggtaagcg cagtgagtgc
     2041 gcgagcgcgt agagccattg ggtaactttc attttgcatt actctagcca attgccgcat
     2101 gatatgggca cgccaagacg cactctcggg agcataacgg agccatcttg ctgcggtgaa
     2161 tatatcgcgc atcagtggct cagggctctt ttcaaaataa ggtcgcacaa aagagtctgc
     2221 atcagggctt tttgtagcta aaaatccaag tgtttgcaaa tagacaggcc actggctatc
     2281 tttcgtcatg ctgctcaccc ccccgttttt cgcaaagcca caggccgcca agtaactgag
     2341 gatcaaggga tgggaaaagg acaaagtttc attttggtgg gaaattagca aaccactctc
     2401 aattaaggct ggaagaacgc gcgaatcccg gattggagct tgaggggatg attttggtct
     2461 ttccgtatca ataggctgcc aattggcatc ttcaatttca actccgggcg ctgcatcagg
     2521 agctggagat ggctcgatct ctgctagcca attctgagcg tcccgcagcg taaatatagg
     2581 ttttcttgtg ttgaccgcat ggcgggttaa gagctccaaa gcaggacgac cttttggtat
     2641 gtcaactgtc acacgttgca aataggcttc gatccaatca gttttatgtg gaccaggtag
     2701 atcgccagcg tatgttgccc aaactttgag agttaattcg agtggagaaa ggatggaatt
     2761 atcgttggtt agccaggcat tgagcaggtt tggatcgagg gcttctgtat ccttagtcat
     2821 tgatccgatg tggttgatcc acatatcgct ccaccgattg ataaactctc ggcgctgttt
     2881 atcgttccag cttgccagag taaggggcac gcacccgatg gcagttaaac catcgaagaa
     2941 gtctggtgtt gcagtggcga taaggcgtgt atcagggtag tggtcgagca aattcttaat
     3001 atattccaca tatatggtta catcggcggg gggcaattcg tctaatccat ccaacaagag
     3061 tagcgttctg cccccagaaa atgcggaagg aagaaacttt tccaggcgtg cctgggggac
     3121 agatggacaa atctgggcga cggcaataac taaaggctct aataaatcgt cgggattaga
     3181 ggatgggagc gtaatatcag cggcatgaag cagtattggc agactatcct taagattgga
     3241 cgggagctgg tctgcttgaa tgatgtccgc acagagttta gccagggtga ccgtttttcc
     3301 gctccccgag tggccgataa tcagcaggtg ggcgtttcct cccagcgctt cgagagcagt
     3361 aaagctgggc gcgccgtaaa aagaagccaa ttctggggca tctggtaggg gaggaagaat
     3421 attctcagca atatctattg aaggtggagt gatgccaggt tcagtcaggg cgggcggtgc
     3481 aagcaggcgg ggggtaaggg agatctcatc cagagagaat agtggcgagg ctagatgtaa
     3541 cccctgagag tgacgcagga gatcatttcg taagcggatc tcggttaccg ttgctgctcc
     3601 caatttggat agttgtgagc gctcgcgcaa ggattggaac aggcgcgcca cccatggacg
     3661 gagtctgcca agtagccaaa tgaggagaat tccggcaatg aaacccagcc agaaggagag
     3721 ccggtctaac tcaaagctgc gcaagaaacg gatcatagtt agccggagta aagtattcga
     3781 ccgcaggatt cacagcggct taattggttt ggagaacggg cagcctgtaa caaagccgca
     3841 tttagtgacg atccgcaggc
//
LOCUS       DBNGSX010000320         4228 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_927561, whole genome shotgun sequence.
ACCESSION   DBNGSX010000320 DBNGSX010000000
VERSION     DBNGSX010000320.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_927561"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            605..2014
                     /locus_tag="AC16CB_09620"
     CDS             605..2014
                     /locus_tag="AC16CB_09620"
                     /inference="COORDINATES: protein motif:HMM:NF009881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3342766.1"
                     /translation="MDLFSHAMHERMKSEAPLAARMRPRILDEFVGQEEIVGPGRLLR
                     RAIESDRLFSSIILWGPPGSGKTTLAMVIANHTRMHFETLSAVLAGKAELKKVIEAAI
                     ERRKLHNRRTILFVDEVHRWNKAQQDALLPHVENGNITLIGATTENPYFEVIGALVSR
                     SRVFQLRPLEDEHVRRILEYALQDDERGYGAKHIKLDEDALEHLIRVAGGDARNALNA
                     LELAVESTPAGDQETIHITLQVAQESIQRRAVLYDKDGDAHYDTISAFIKSVRGSDPD
                     AALYWLAKMLYAGEDPRFILRRLIILAGEDIGLADPLGLVIANAAAQSFDYIGLPEGI
                     YPIVEATLYLATAPKSNTATSYFKAFQLIEDEGITAVPQHLQDANRDAKPLGHGQGYQ
                     YPHEDPEHFLPQQYLPHPLLGTYFYKPSSQGYESQVIDRLSRWRDAQRKALGITQTTE
                     LPEPSQDEIQEIKHKHKAG"
     gene            2019..2636
                     /locus_tag="AC16CB_09625"
     CDS             2019..2636
                     /locus_tag="AC16CB_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018770996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR3342767.1"
                     /translation="MPTILLIRHGENDFVKKGIMPGRMPGIHLNDNGRAQAQAVAEKL
                     RQAPIKAIYTSPLERAAETAEYLAKALGQETIVRPGLMESDIGEWQGKSHKSLRRLKV
                     WRIVQSTPSLFRFPGGESFAECQARMVGEIEALRAQYEEKDIFVCVSHADPIKLAVAY
                     YLGLPLDLFQRLFIAPASITALQVGQMGSYLLTLNYDLSFNLSKP"
     gene            2678..3205
                     /locus_tag="AC16CB_09630"
     CDS             2678..3205
                     /locus_tag="AC16CB_09630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 domain-containing protein"
                     /protein_id="HIR3342768.1"
                     /translation="MPTKEIDLRPVSHITTDAIGPPGKRIFYIQGWQEERTVTLIVEK
                     IQIQSLAVGLEQFLAEIYERFPNLTKASSEYVEENMHIHPPLDPLFRTGELGLGYDAE
                     NDLVVLIARELLAEDQDPEEASVVRYWCTRDQIRALCHWGMEVASRGRPLCPQCGQPM
                     DPEGHFCPKKNGHKH"
     gene            3289..4047
                     /locus_tag="AC16CB_09635"
     CDS             3289..4047
                     /locus_tag="AC16CB_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HIR3342769.1"
                     /translation="MMSSISPNDTFTALQKGEITLRGEFMWGSNYTYLAQLEHAGEIL
                     TAVYKPTKGVRPLWDFPSPSLARREVAAYLISEALQWELVPPSAYRKDAPLGPGSVQL
                     YIDHDPEYHYFNFTPEDRQRLRPTVVFDLLANNADRKGSHILVDTNHHMWLIDHGICF
                     HVDEKLRTVIWDYAGEEIPAQLLADVKRLRDKLLPLNKQHPEFESSDLVRELGKYLNR
                     EEIAALIQRANRILENGCYPNPDLSRRQYPWPPV"
BASE COUNT         1037 a         1205 c         1070 g          916 t
ORIGIN      
        1 ggaatgccgt ccaccgtgtc tccgcgaatg tctagattaa ctccagcagc tttcgccgcc
       61 tcagtaatcc gtgcctccaa gaggacggta gagaatccaa aggcgcaaac gaggagcact
      121 ctgatggcct tagattcctg tgggcttccc atgtgtacaa gctccttaca cggttcggtt
      181 acgcgttttc taagactatg acgcttagat cacatgtgaa tgtttcccgt tagatgattg
      241 gacgatgtca gtttgggcca tgcaccgctc gacaagtagc ggttgtcata ggatagaaag
      301 tgctgcctaa cagtcgagtt cggccgtgta catgaagcga aacagaaagt atttcggctg
      361 caagactgtc gttcgctgcg attcagcagc tcaataacta acagacctct acgacataaa
      421 acacaatagg ctgcacaata tttttatgag tattttggtg agagtatagc acaacatcag
      481 aaaagtcttc atgccaggac aagctctacc cgcgtgctca agaatagtcc aaagtgatgc
      541 ccagcccgtt agatgagcat ttcttcgctt gcccctgact tgatggggcg ttataatctc
      601 ctccatggac ctcttctccc acgccatgca cgaacgtatg aaatccgaag cgcctctggc
      661 ggcgcgaatg cgccctcgca tcctggacga gtttgtcggg caggaggaga tcgtcgggcc
      721 ggggcggctg ctgcgccgcg ccatagagtc cgaccgcctg ttctcgtcca tcatcctgtg
      781 gggcccgccg ggcagcggta agaccaccct ggcgatggtc atagctaacc acacccgcat
      841 gcacttcgag actctttcag cagtgctagc tggcaaggct gagcttaaaa aggttatcga
      901 ggctgccatc gagaggcgca agctgcacaa ccggcgcact attctcttcg tggatgaggt
      961 gcatcgctgg aacaaagccc agcaggacgc tctgttgccc cacgtggaaa atggcaacat
     1021 taccctgatc ggcgcgacca ctgaaaatcc gtactttgag gtgatcggcg cgctggtctc
     1081 gcgctcgcgc gtcttccagc tccgccctct ggaggacgag catgtgcgcc gcatactgga
     1141 atatgctttg caggacgacg aacgcggcta tggggcaaag cacatcaagc ttgacgaaga
     1201 tgctttagag cacctgatcc gcgtagctgg aggtgatgcc cgcaatgcct taaacgcact
     1261 ggaactggcg gttgagagca cccccgctgg cgatcaggag acgatacaca tcacattgca
     1321 ggtggcgcaa gaatccatcc agcgccgtgc tgtgctctac gacaaggatg gcgatgccca
     1381 ttatgacacc atctcggcat tcattaaatc tgtgcgcggc tctgatccgg atgccgctct
     1441 ctactggctg gcgaagatgc tttatgccgg ggaagacccg cgcttcatcc tgcgcaggct
     1501 gatcatcctg gctggggagg atatcggtct ggctgaccca cttgggctgg tgatcgccaa
     1561 cgctgccgcc caatccttcg attatattgg cttacccgag gggatctacc ccatcgtaga
     1621 ggccacgctt tacctggcca ccgcaccgaa atccaacaca gccacctcct atttcaaggc
     1681 cttccagctc atcgaggacg agggcattac tgccgtcccc cagcacttgc aagacgccaa
     1741 ccgcgacgcc aagcccctcg ggcacgggca gggttaccaa tatccgcatg aagatccaga
     1801 gcacttctta ccacagcagt atcttcccca cccgcttttg gggacctatt tctacaaacc
     1861 gtcatcacag ggttacgagt ctcaggttat cgataggctt agccgctggc gagatgccca
     1921 gcgcaaagcg ttgggtatca cccagacaac tgagcttccc gaaccatccc aagacgaaat
     1981 ccaagagatc aagcacaaac acaaggcagg ctaagtaaat gcccacaata ttattaattc
     2041 gacacgggga aaacgacttc gttaagaaag gaattatgcc agggcgtatg ccgggcattc
     2101 acctcaatga taacgggcgc gctcaggcac aagccgtagc agaaaagcta agacaagcgc
     2161 ccatcaaggc aatctacacc agcccactgg aacgggctgc ggagactgcc gaatacctgg
     2221 ccaaggcatt aggtcaggag accattgtac gaccaggtct gatggaatcc gatatcggcg
     2281 agtggcaggg taaaagccat aaaagcctgc gccgcctgaa agtctggcgc atcgtgcaga
     2341 gtacgccctc gctcttccgc ttccctggcg gcgaatcatt cgccgaatgc caggcgcgca
     2401 tggttggaga aattgaagcg ttgcgcgccc agtacgaaga gaaggatatc ttcgtttgcg
     2461 tgtctcacgc cgacccgatc aaattggcgg tggcatacta tcttggcctc cccctggatc
     2521 tgttccaacg actatttatt gcaccagctt cgattaccgc cctgcaagtc ggccaaatgg
     2581 gcagctacct gcttacgctg aactatgatc tttcttttaa cttgtccaaa ccttgattct
     2641 ggtatactac gctcagtatc ccaggcagga gagcctaatg cccactaaag agatcgacct
     2701 tcgtccggtc agccacatta caaccgacgc tatcggcccg cctggtaagc gtatttttta
     2761 cattcagggc tggcaggaag agcgcaccgt aacactgatc gtagagaaga tccagattca
     2821 atccctggcg gtgggtctgg aacaattcct ggcagagatt tacgagcgtt ttcccaatct
     2881 caccaaggcc tcctcggaat acgtagagga gaatatgcac atccatccgc ccctggatcc
     2941 gctcttccgg actggcgagt tagggctggg ttacgacgca gaaaacgacc tggtggtcct
     3001 catcgcacgc gagctcttag ccgaggatca agaccctgaa gaggccagcg ttgtacgtta
     3061 ttggtgcaca cgtgatcaaa tccgcgcctt gtgccattgg gggatggaag tggcctcgcg
     3121 cggccgtcct ctctgcccac aatgcgggca acccatggac cccgaaggac atttctgccc
     3181 caagaaaaac gggcataaac attgatcctt cttaaatcaa caatacagca attacgaacg
     3241 aatggatata aatgatcttg aataatttca ttcgttgatt tacgcctgat gatgtccagc
     3301 atatcaccca acgacacatt caccgccctg caaaaagggg agatcacgct gcgtggcgag
     3361 ttcatgtggg ggtcaaacta cacctatctg gcccaactgg agcatgcagg agaaatttta
     3421 accgccgttt acaagcccac caaaggcgtg cgccctctgt gggatttccc ctccccatct
     3481 ctagcgcgcc gcgaagtagc cgcctatctg atcagcgaag ccttgcaatg ggagctagtg
     3541 cccccttcgg cctaccgcaa agatgccccc ctgggtcctg gttctgtcca gctctatatc
     3601 gatcatgatc ctgaatatca ttacttcaat tttacccctg aagatcgcca gcgcttgcgc
     3661 cccacagtag tttttgatct actggcaaac aacgctgacc gcaagggcag ccatatccta
     3721 gtggatacca accatcacat gtggttgatc gaccatggca tttgcttcca tgttgacgaa
     3781 aagctacgca cggtaatctg ggattatgct ggagaagaga tccctgccca actgcttgcg
     3841 gatgtcaaaa gattacgaga taaattactt ccgctaaata aacagcatcc cgagttcgag
     3901 tcatctgatc tagtgcgaga gcttgggaaa tacttaaaca gagaagaaat cgcagcgctg
     3961 atccagcgcg caaatcgcat acttgagaat ggttgttacc caaatcctga tctcagccgg
     4021 aggcagtatc cctggcctcc cgtataggag aatgatatgc cacactacaa actcgcattt
     4081 ttaggctttg gcaacgttgg acaggctctt gcacgcctgt tgctcagaaa gcaagaaacg
     4141 atccaatcgc aattcgggat taccttcaac gtgaccggta tcgccaccgg ccgccacgga
     4201 acagccattg accccggtgg gatcagcc
//
LOCUS       DBNGSX010000321         4179 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1896861, whole genome shotgun sequence.
ACCESSION   DBNGSX010000321 DBNGSX010000000
VERSION     DBNGSX010000321.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1896861"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..1140)
                     /locus_tag="AC16CB_09640"
     CDS             complement(190..1140)
                     /locus_tag="AC16CB_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342770.1"
                     /translation="MKRIPSFLSILILLALGACTLRMPGVEAPSAIVSTPETLTGEVV
                     APTEVIAPAQLAAPTDTAALPTVAMPAAALPTPYPAPETQAPPPPTSEPTATQPAPTA
                     TPTPVITIDPNTAYGEPDYVNRMQFPNLSEWAPPETGELPNNRNIRLQFKDGELYVTG
                     KRINFSTWWFSYHNLSDAYIEMTFETEDCSGEDAYGLIFRGPPHLAGESYGYAVSFTC
                     SGNLWVLRLDGVNPWEAETLIDMEDIYAIRTGKDEENVIGVRAEGERFVIYANGNQIA
                     EVEDDHFEKGRVGVFVRAGRPGAYTYRVTNFAYWILGEEE"
     gene            1499..2341
                     /locus_tag="AC16CB_09645"
     CDS             1499..2341
                     /locus_tag="AC16CB_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342771.1"
                     /translation="MDIYLATERAFHLVPQFTQEVARDRLEQKKTSLVAGTVGSMISR
                     PRPDEIQLVSVESRLEAFWLIDVFVRTTYDNNRTYTIPVSGPEVKQVTALGQDLQVTS
                     SPKGGASFTLNAVDHCIEEYRGSYTFDGAGTKADMSKYQSFTKVEISDLGQFAPADVL
                     VVPPKANATTVMRAVLADVIKPVQAQVIHEERVNVEALDLYFRPVYAFEYEWTTKGKR
                     AVMEFDALTGDARSGGKKLSDQIKSMVTRDLVFDVTADAVGMIVPGGSIAVKLIKAVV
                     DRGK"
     gene            complement(2359..3870)
                     /locus_tag="AC16CB_09650"
     CDS             complement(2359..3870)
                     /locus_tag="AC16CB_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342772.1"
                     /translation="MQSTHIGSKNLGTNTIQRALLAACIFAGLAILRDSLILWAFSFG
                     YYRDSNLYVSLGRTLFQKPSPFSAGIVTFPYPFLNAITFSSSNPVGLVWLQILLGGVA
                     LGVLTYVIAKKNLSLAIVVGTLLVFDFIWGAVNRNVMKEGPFVSFNILSLAILVSHYD
                     RRDSLSDWELMLVGILYGWTFIFRPSSLFLAILIPPVYLWLTRSWRKTGFLLGGFIII
                     YLVLGLFNLWSVGRFKIFGESGYYTGSPLFVYRLFSPENGPASQELGSKFDSCMPDFD
                     YVNAMETSAGGTTNNELLYGKFIPCLYSFGMSLNEASDLITQAYIESIIRNPVNFAGI
                     VFREGITFLKYATPNILRFYLKPELNNRCEDYTWCENIRESRYSWGERIPLARLYEKV
                     ATKVFQIYLLPIRPLTLVFSGNPYLPFAAAWIVLIAFVFVITQGSARFLVISSLLFIH
                     YVIITVVSGYGFLARYSSVLSPFYIIVSAVALYSSARLLWSMFGRLRKGRGER"
BASE COUNT         1105 a         1039 c         1070 g          965 t
ORIGIN      
        1 caggggagtg gatagccttt atttctaatc tcttgcggac ctttttagca tagacaagcg
       61 tcgcccaatt aatcccgact atctatactc gatgtaatcc ctgccggatt ggctcactcc
      121 ggtaactgcc gcggccggct ttccctcgat accctgtagg ccaactcggg atactaccgg
      181 tctattgatc tattcttcct cccccaggat ccaatatgca aaattcgtga cgcggtaggt
      241 gtaagcccca ggcctacctg cccgcacgaa cacgcccacc cgccccttct cgaaatgatc
      301 gtcctccacc tctgcaatct gattgccatt agcatagatg acgaaacgct cgccctcggc
      361 gcgcacccca atcacatttt cttcatcctt cccggtgcgg atcgcataaa tatcttccat
      421 gtcaattagg gtctcagcct cccagggatt taccccatct aggcgcaaca cccataaatt
      481 tccgctacaa gtgaacgata cggcgtaacc gtacgattcc cctgccaggt gtggtgggcc
      541 gcgaaagatg agcccatatg catcctcgcc agagcaatct tcggtctcga aggtcatttc
      601 gatataagca tcactcaagt tgtgataact gaaccaccag gtgctgaaat ttatgcgctt
      661 gcccgtgacg tacagctcac catctttaaa ctgcaagcgg atgttgcggt tgttgggtag
      721 ctcgcccgtc tcgggtggcg cccactcact cagattgggg aattgcatcc ggttcacgta
      781 atctggctct ccatatgccg tgttcgggtc aatggtaatg actggcgtgg gggttgccgt
      841 aggcgccggt tgggtggctg tgggctcgct ggtgggtggc ggaggggctt gtgtttcggg
      901 cgctgggtat ggcgtgggca gcgctgccgc cggcatagcc acggtgggca gcgctgcggt
      961 atcggtgggg gctgccagtt gggcgggggc gatcacttcg gtaggcgcaa cgacttctcc
     1021 ggttagagtt tccggcgtgg agacaatcgc gctaggtgct tctaccccgg gcatcctcag
     1081 tgtgcacgcg cccaaggcaa gcaggatcag gatggataga aaggatggaa ttctcttcat
     1141 gaggttttcc caatctatag tttcttttag ctttattttg gcatcgacat cctgtcgcta
     1201 cctggaatgg agataagtca attgatgatt gatataggtc ttactgcccg caggagaaca
     1261 aaaagattat aacatgtggt cgaagctatt accttacagt atcacaagcc cgaggctaaa
     1321 tccctgtttt gtggacttgg acccgcacac aagggctccg ggcagataaa ttagccgtta
     1381 cacgatctgc tggttttatg tcataattat cgttatagcg ctgcccagat cgtcttttca
     1441 tagacaaatt ccgctgggca gccgcgccga gaatccaaaa tacagggcag gatcccatat
     1501 ggacatctat ctcgctactg aacgagcttt tcatctggtc cctcaattca cgcaggaggt
     1561 ggcacgagat cgcctagaac agaagaaaac cagccttgtc gctgggaccg tggggtcgat
     1621 gatctcccgt cccaggcccg acgagattca attggtgtct gtcgagagcc ggctggaagc
     1681 tttttggctg attgacgtct ttgtgcgcac aacgtacgat aataaccgca cttacactat
     1741 cccggtaagc ggcccggaag tcaaacaggt tactgcgctg gggcaagact tgcaggtcac
     1801 ctccagcccc aagggcggtg ccagctttac gctcaatgca gtggatcact gcatcgaaga
     1861 ataccgcggt agctacacct tcgatggagc tgggacgaaa gcagatatga gcaaatacca
     1921 atcatttaca aaggtagaga tctcggactt gggacaattt gcgccagcag acgtgttggt
     1981 agtgccgcca aaggcgaatg ccacgacagt catgcgggcg gtgctggcgg acgtgatcaa
     2041 gccagtgcaa gcccaagtga tccatgagga gcgggtcaac gtggaagccc tcgatctcta
     2101 tttccggccg gtgtatgctt ttgagtacga atggacgacc aaaggaaagc gtgcggtgat
     2161 ggagttcgat gcactcaccg gggatgcaag atcagggggg aagaagctga gcgatcaaat
     2221 caagagcatg gtcactcgcg atctggtttt tgatgtcacg gcagacgcag tagggatgat
     2281 tgttccaggg ggaagcatcg cggtgaaact gatcaaagct gtggtagatc gcgggaaatg
     2341 aaactggtta catccatatt agcgctctcc cctgcccttg cgaagccgac cgaacatgct
     2401 ccaaagcaat ctagccgatg aatacagggc aacagcggat acgatgatat agaatggcga
     2461 aaggacagac gaatagcgcg ccagaaagcc gtatccgctg acaactgtga tgatcacgta
     2521 gtgaataaat aacagactgg aaatgaccaa aaaacgagcg ctgccttgcg tgatgacaaa
     2581 cacaaaagca atcaacacga tccaagcagc cgcaaatggc aggtaggggt tacccgaaaa
     2641 taccagcgta agcgggcgaa tcgggagcag gtaaatctgg aataccttcg tggccacttt
     2701 ttcatataat cgcgccaaag gaattctttc gccccaggaa taacgcgact cgcgaatatt
     2761 ctcgcaccaa gtgtaatctt cgcacctgtt attcaattct ggtttcagat aaaacctgag
     2821 gatattgggg gtagcatatt tgaggaatgt aatcccttct cggaaaacaa tccccgcgaa
     2881 attaaccgga tttcggatga tcgattcaat atatgcctgc gtgataagat cggatgcctc
     2941 atttagactc ataccgaagc tatacaaaca aggaataaat ttgccataga gcagctcatt
     3001 gtttgtcgtg cccccagcag atgtttccat cgcgtttaca taatcgaaat caggcataca
     3061 actgtcgaat ttgctaccca attcctgtga agccggtcca ttctctggag aaaaaagccg
     3121 gtacacaaac aagggactcc ctgtatagta gccggattcc ccaaaaatct taaatctccc
     3181 cactgaccaa agattaaaca gtcccaatac caggtatatg attatgaagc cacccaagag
     3241 aaatcctgtt ttgcgccatg agcgggtgag ccatagatag acgggtggga tcaaaatcgc
     3301 caggaacaag ctcgacggtc ggaagatgaa tgtccagcca tagagtattc ctacgagcat
     3361 tagttcccaa tccgatagcg agtcgcggcg gtcgtagtga ctgaccaaaa ttgccaggct
     3421 caagatgttg aagctgacaa aaggaccttc tttcatcacg ttacgattta cagcccccca
     3481 aataaaatca aataccagga gggttccaac tacaatggct aacgagagat tctttttcgc
     3541 gatgacatag gtcaaaacgc ctagggcaac tcctcctaag aggatttgca accagacaag
     3601 accaactgga ttggacgacg aaaaagtaat tgcgttgagg aaggggtagg gaaaagtaac
     3661 gattccggcc gaaaatgggc tgggtttttg gaagagggtt cgacccaagc ttacatacag
     3721 gttcgaatca cgataatagc caaatgagaa tgcccacaat ataagcgaat ctcgcaaaat
     3781 agccaaaccc gcaaaaatgc aggcagccaa taatgcccgc tggatggtat ttgttcctaa
     3841 attcttggag ccgatgtggg ttgattgcat ttgcgaagga tattcaacca gggaaatcag
     3901 tagtctcaaa gataaaaaaa ttataacatc gcctgcctta ctgaaggatt gagattagta
     3961 atagctcttc tgagaaaaat gtccgacttg cgtcaataaa aatagctccc cacccacttg
     4021 gagcgatttc gctttgcctg ggatatatta gcaaagctcg gccggataaa cctcggccga
     4081 gcttcgtata actatttcac ctcagactgt taggaagcag cctgcgggga cttatcggtg
     4141 tttacggtgt ttgaaagacc agtctccctt cgatggccg
//
LOCUS       DBNGSX010000322        15514 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_233822, whole genome shotgun sequence.
ACCESSION   DBNGSX010000322 DBNGSX010000000
VERSION     DBNGSX010000322.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 15514)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15514)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15514
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_233822"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="AC16CB_09655"
     CDS             <1..431
                     /locus_tag="AC16CB_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342773.1"
                     /translation="PTEFFGEELEHNWCYYYQKAELAAQFGAWQQVAELGDEALAKGF
                     SPGNSHEYLPFIEGYARSRRWENAIARTQAAFTANELIAPRLCSLWKRILAQDVPPAD
                     FKSSIQQMQNLLQCNQAPNENSLSIKYSSTSIVVFDRTYP"
     gene            718..1899
                     /locus_tag="AC16CB_09660"
     CDS             718..1899
                     /locus_tag="AC16CB_09660"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008443 - phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR3342774.1"
                     /translation="MSKRVAILTGGGDVPGLNMCLKSLVYSLIDSGFEPIGVRKGWDG
                     FIQYNPHDPSSYSESFIELTKNMVRPIDRTPGSFLHSSRLDPRYVPRSLVPQFLRKSK
                     EEIQDLTNHIKDVVQRLGYQCLITIGDDDMLHYAAYLSEQGVPVIAIPKTIHNNIHGT
                     DYTLGFSTGMARGVSFMHELRALAGSREQVFVVETFAAKSGYSTLLMSFLGGVDRVII
                     PEVPYDPELLAQLVILDKKQTPANYALVLVCDGSSVREDKIEKFASVLPTGDGTEVMR
                     AHLSGDQKLVGSGMVAAQLLRHLLGEETFLQQLTYLLRTGPPDGQDLLGAANFAIIAS
                     RLAQEGRYGRMAAFVQDQMWTDVDLNLAIEGVRTVDVDAWYDTKEYRPKLSIIWSAGE
                     V"
     gene            1928..2656
                     /locus_tag="AC16CB_09665"
     CDS             1928..2656
                     /locus_tag="AC16CB_09665"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020485422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3342775.1"
                     /translation="MSSANGNNRLVQIVDLVKSFPVGGGEVTILKGLSFDVKQGEFVS
                     IVGPSGNGKSTLLNMITGIDRPTAGQVIVTGLEINKMSENQLAAWRGKHVGIIFQFFQ
                     MLPALSLLQNVILPMDFAKKYSPRERRERGMHLLEIVGLEDQAHKLPSMVSGGQQQRA
                     AIARSLANDPDLLVADEPTGNLDTRNAGDVFDLFLKLVDQGKTLLMVTHDKELSRRVP
                     RVVEIIDGKITRDEFVGGAVWTGY"
     gene            2668..5064
                     /locus_tag="AC16CB_09670"
     CDS             2668..5064
                     /locus_tag="AC16CB_09670"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3342776.1"
                     /translation="MGVLRYKIYRDLWVNFGRTLQVVLIIAIGAGAIGMIVGTRYLVI
                     SNMQSAWRAMNPATINLFIGPPIDEDDLIALESEDGVVEIEGLGDSNIEWRLNPDDEW
                     QLGGLTFRANYEEQKLNLLELVEGDWPSGKFVSIGQGDDQFFGVPKNGKVYLRLDEKE
                     YTIQTDGVVYNQLQQPAYFGGTAQFYATQEEYERIVGDRDFSQVLVTGVEYDELKLAE
                     LGDRLQDRIEKMGSDSSRMILDPNEHFFQPQLDGIFLILGVMGGLALALGLLLVYTTI
                     NAIITQQVDQIGIMKAIGARTWQILRLYLTSIFFYSILSLLIALPLGLGGAWSITSWL
                     VTGFGADPGSFDFSREAVIVMLLIVFLAPFLASLLPIFNGARITVREAISTYGLSTGA
                     GLLERLLAKAKAMSRMLLMVISNTFRNKWRVVLMQITLVLSGLIFMMVVSLRDSVVYS
                     VKDILFSILNANITFLFEEPQRIHYLEDLTMKYPGVKAVEMWGFAGVNMRPAGQEASE
                     DDELASVWGVPLPTQTYGYQLRSGRWLEPTDTHAIVMNQQLADDVGVTVGDWITVKYD
                     EKNEVNWQVVGLVFDPILTNVALASREVLLHDLRFVGRAQSAWIQTERDDMQTEIAVA
                     KGLREFYKQNGVEVSLRKGVFGMGGDSTTQAANALIAQFNTFIALPGLLAVIIAAVGG
                     IALSGVLTLSVLERRREIGVMRAIGASSFTIARLFIGEGLLLGWMSWLIALPLSIPVG
                     RLMPQAVGSALQLDLVYKYTPTGAVLWLVIITFLSIIASWLPARGATRISVRESLAYQ
                     "
     gene            5079..6263
                     /locus_tag="AC16CB_09675"
     CDS             5079..6263
                     /locus_tag="AC16CB_09675"
                     /inference="COORDINATES: protein motif:HMM:NF052757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR3342777.1"
                     /translation="MKSKLFTLIIIIALGSLFLSACGSIGAPADDAAPTEIPIVITDT
                     DVVAEGRLVPNDSANLSFKAAGQVTEILITEGDTVEAGQVIARLDNSEQLQSAIANAE
                     AELLNAQLARDALFDNAKVNSAAALQAIADARDAVRDSDRYLNNLNAGSRDTDIDLAK
                     ADVAILKDQLDEAQEDYKPYENKPEDDLKRATFLARFAQAEQLYKDAVRKLNNLQGEP
                     SKIDLDIAEANQAVAEAQLLLALQNYDEIASGPDPDDLAAVEARIKAAEAGLVSAQAT
                     LNNIELVAPFDGKIVDLVITTGEQVAPGVTAAVLADFSQWIVETDDLTEIEVPEISIG
                     QSVTIVPDALPDLELTGMVERISDVFEEKRGDITYTARIKLNEENELLRWGMTVVVTF
                     EK"
     gene            6400..7617
                     /locus_tag="AC16CB_09680"
     CDS             6400..7617
                     /locus_tag="AC16CB_09680"
                     /inference="COORDINATES: protein motif:HMM:NF052757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR3342778.1"
                     /translation="MTGVSLLLIFGSILLPACSAVGIGPSSTPTPTVTPEVESVIQVS
                     AEGQVVPRHHANLSTAVSGQVTELLFREGEQVQEGDIILRLGDREQYQADQAAAELEL
                     LSAEQALEELQKNVGVELAQTRQALALANQERSKIFDEYQNLSKPVPQDVLDQAYASV
                     LMAEKRLQKAGEDLEKMEKRYKNKSNIMWQFLNKKQFKDLLEQMRRARLSAEDRYYDV
                     LDRYNDLKNHPDEIELAKATADLGLVDARIADLQGDIAMLEKGPDSDKVASAEARRKA
                     AQVALEAAQLALNNSEIEAPFSGQIAEMNVTQGEWVEANQPVILLADLNHWEVQTDDL
                     TELDVPIVSLGQTVTIKLDALPEVELKGKVDAIKDLSEEKRGDVTYTVTILLDEVDPR
                     LRWGMTAAVEFSD"
     gene            complement(7718..7963)
                     /locus_tag="AC16CB_09685"
     CDS             complement(7718..7963)
                     /locus_tag="AC16CB_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HIR3342779.1"
                     /translation="MDYGMIGKIEKAKRYAEQRDRIRIDSLTVTFEGENNPHTVSLEN
                     GVWQCDCDFFQTRDRCSHTMALEIILDGMLPEIVFRD"
     gene            complement(8116..8499)
                     /locus_tag="AC16CB_09690"
     CDS             complement(8116..8499)
                     /locus_tag="AC16CB_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR3342780.1"
                     /translation="MNRFLIRWGINAVALYVAVALVPGIQPQSTNWLAFIWLALIFGV
                     VNAFLNPLLKFLTCPLILLTLGLFTLVINTGLFYLAGNIGSSFGVGFTVDNFWSALLG
                     SIVVSVISIVLSLVFKDELKRKYLN"
     gene            complement(8748..9512)
                     /locus_tag="AC16CB_09695"
     CDS             complement(8748..9512)
                     /locus_tag="AC16CB_09695"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIR3342781.1"
                     /translation="MGKIKAVFFDQDGVIIDTERDGHRVSFNMTFKEFGFTDEWSVNY
                     YHELLQIAGGKERMKHHSLTKGFNPPVPPDEIDELVKTMHKRKTALFVELIESGQLPL
                     RPGVHRFMKEVVEAGLMIGVCTTSNEQSARAITGKILSDIKFDLVLAGDVVSKKKPDP
                     EIYNLALSRTGLKPDECFVVEDSKNGVLAAKAAGMPTVVTTNYYTEKEDVSSGDIIVT
                     SLGDPDGEKGVLTKGGDGLKFDGVLHVQQLVEYFEK"
     gene            complement(9621..10724)
                     /locus_tag="AC16CB_09700"
     CDS             complement(9621..10724)
                     /locus_tag="AC16CB_09700"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR3342782.1"
                     /translation="MKSIEEYYVPWVKGIPMYISGHIELAWRQPELHRMMSNENPNPP
                     SEKVLATIDKYARLGHRYPDQGLVVRGKIAEINGLDGPDNVMLGNGSSEVFDNIFRVF
                     LAPGEEVIQHTPCFGIYKLRCNILGGKLVSVPMVYKDNQLLYDPDSILKAVTDKTKVI
                     VIANPNNPTGNFMDAKEFVRIAETGIPFVVDEAYIEYAGLGMSQVGLTKKYKNVIITR
                     TLSKAYGLAGLRFGYALGDKSVIGQISAALLPWNVGTIPMWAALAALEDTEALEYRVK
                     FNNSEVDFIQESLSDIPGLVIFRSQANYVLFDAGGTGKRGKDMVAYAEQKGLIFRPDS
                     DKYGSDGWFRITIGSKEENRMAVAIVREFLTKS"
     gene            complement(10818..11693)
                     /locus_tag="AC16CB_09705"
     CDS             complement(10818..11693)
                     /locus_tag="AC16CB_09705"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0030388 - fructose 1,6-bisphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HIR3342783.1"
                     /translation="MSIVNAKEILVEAAKGKYAVGAFNITDLIQLEGVVDAAVEKKSP
                     LIIQTSVKPSKFLGTKVLVAIYKTIAESAPIPICLHLDHCTEVDYCKTCADAGYTNIM
                     IDASKQTFEENIRQTKEVVDYCHSVGDISVEGELGTVSGVEDQIKVAEDEAALANPVQ
                     GVEFVERTGVDIFAPAIGTAHGVYKTKNPKVDFERLGKINQMLNGGGVKTPLVVHGGT
                     GLPEDYIQKLIEMGGAKFNVSTELKHTLINSTYDYITAHREEYDPGKVDVAVRDAIRQ
                     AVGHWIDMLGSAGKA"
     gene            complement(11810..12736)
                     /locus_tag="AC16CB_09710"
     CDS             complement(11810..12736)
                     /locus_tag="AC16CB_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR3342784.1"
                     /translation="MGLTIEQAIARVPFLAEANDIKITSLSGGITNLNYKIETDGKAY
                     VLRITGEGTDALGIRREVEYQANLAAGLLGVAPEVVYFIEPEGYLLTRYVKGKHIPPD
                     VITRPENIVRVVRKIRLFHRNAPQLDNEFNVFRRVEMLTATSKRNNCKFPFDFDWIMQ
                     KMREAEGALLKDPYIPTPCHDDLLNLNWLDEDVPGEIGEIRLLDWEYAGMGDIFFDLA
                     NFSHHHRLNDDQVRLLLQEYFGEVTPKNFARLKIMWPMSEIHEAMWGTTQTGISKLDE
                     DFQGYADLWFGRARQHVTDPRWDKWLVDVAGK"
     gene            complement(12808..13536)
                     /locus_tag="AC16CB_09715"
     CDS             complement(12808..13536)
                     /locus_tag="AC16CB_09715"
                     /inference="COORDINATES: protein motif:HMM:NF019322.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3342785.1"
                     /translation="MFELTKIEGIPMYVWIREALRKEITGGVLKRGEKLPSEHDLASK
                     FGVSRMTVRHGIAELIDEGLLYRRHGVGTFVAFPHLERDHTRLTNFFDRTESEDIHAK
                     ATVLDLEVIPAKQKIAKALDITVGELVIRVKTLRFADNVPITIHDAHIPHKLFSKLID
                     ENLEVQNLWSLFEQSGYKVKRAIQRIEAKEATKELAKLLGIREGASVLYKERTVYADD
                     GTPVEFTYCYNRGDIYSLTVSLER"
     gene            complement(13759..>15514)
                     /locus_tag="AC16CB_09720"
     CDS             complement(13759..>15514)
                     /locus_tag="AC16CB_09720"
                     /inference="COORDINATES: protein motif:HMM:NF013720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GcvT family protein"
                     /protein_id="HIR3342786.1"
                     /translation="LYIITKPIEGVRHEFPTLRDPDLLVYWREEVGGLVTGGYERNPE
                     PFGLKGIPRDFKYQLLPPDWERFAPLMENSIKRVPVVETAEIIQLLNGPEGFTPDGEF
                     LLGPTSVKGFWVACAFCAHGLAGAGGIGKVMAEWIIDGQPEWDVWRLDVRRFGTNYAS
                     QDYTVARTIETYSMYYDIHYPGEERLSRRNLRISPTYYRLRDLGALFGEKTGWERPNY
                     FKPYEELAVHGHEPTGWARHNWSRAIGYEHLMTRQNAGLFDETSFNKFEVRGPGALAF
                     LNYVCANEIDVPVGSVVYTECLNKRGGIECDFTVTRLAEERFFIITGTAFGQHDMSWL
                     RLHMPEDGSVTIEDVSSSYTCIGMWGPKARAILQKTTSADVSNAAFPYMTAQSITVGD
                     VPVLASRVTYVGELGWEFYCPIEYGLRLWDTLWEAGQPEGMVAAGYKAIDTLRLEKAY
                     RYWSGEISPDYNPYEAGLGFAVKLNKGDFLGREALLKQKESGLKRKLCCATLDDKRTI
                     ALGKEPIRLLGTDNIVGWVASGGYGYSVEESIVFAYLPIEHSQPGTQLEIEFFGAQIV
                     ATVRQMPLWDPKGERIRA"
BASE COUNT         3698 a         4223 c         3985 g         3608 t
ORIGIN      
        1 ccccaaccga gttctttggc gaggagcttg agcacaactg gtgttattac tatcagaagg
       61 ctgagctggc tgctcagttt ggagcatggc agcaggtggc agagcttggt gacgaggcac
      121 tcgccaaggg attttcaccg ggcaactcgc acgaatatct gccttttatc gaaggctatg
      181 cacgcagcag gcgatgggaa aacgccatcg cgcggacgca ggctgccttc actgccaatg
      241 aattgattgc cccgcgcctt tgtagtctgt ggaagcgtat tttggctcaa gatgtaccac
      301 cggcagactt taaatcgagc atccagcaaa tgcaaaatct gttacaatgt aatcaggctc
      361 ccaatgagaa tagcctttcg atcaaatact cctctactag cattgtggtt tttgatcgca
      421 cctatccata aacttcatcg tgaattttta acatatctgg ccataacctt cagcagattg
      481 gcgatttaac gtcctcagct tggctttggc gatgaaggcg catattctca cacaattctg
      541 ttactagcat ttgacctaaa gggtgggttt gaaatgagcc agataacaat tgtaaatcta
      601 ctactacatg cagccctgcg cgacccggct taaataatct cgtatataga caagattgag
      661 ttgcattaga aatgagcaac tctcttgtcc aagccacttt aacataggag aatgaacatg
      721 agcaagagag ttgccatact gaccggtggc ggtgatgtcc ctggcctgaa catgtgtcta
      781 aaaagcttgg tttacagcct gattgattcg ggcttcgagc ccataggcgt gcggaaggga
      841 tgggatggat ttatacagta caacccgcac gacccgtcct cctatagcga gtcctttatc
      901 gagttgacca agaacatggt tcgccccatt gaccgcacac ccggatcctt cttgcactcg
      961 tcccggcttg acccccgtta cgtgccgcgc tccctggttc cacagtttct gcgtaaatcc
     1021 aaagaagaga ttcaggacct gacgaaccac atcaaggatg tcgtgcagcg gctgggttac
     1081 cagtgtctaa ttaccatcgg ggatgacgac atgctccatt acgctgccta tctaagcgaa
     1141 caaggcgtgc ctgtgatcgc tatccccaaa accatccata acaacattca cggcaccgat
     1201 tacacacttg gcttcagcac cggcatggcg cgtggcgtgt ctttcatgca cgagctgcgt
     1261 gccctggcgg gctcgcgtga gcaggtcttt gtggtggaga ctttcgccgc caaatcgggc
     1321 tacagcaccc tgctcatgtc attcctggga ggcgtcgacc gggtgatcat ccccgaagtg
     1381 ccctacgacc cggagctatt agcccagctc gtgatcctgg ataaaaaaca aacgcctgcc
     1441 aattacgccc tggtgttggt ctgcgatggc tcaagcgtta gagaagacaa aatcgagaaa
     1501 ttcgcttcgg tgcttcccac aggggatggg acggaagtaa tgcgcgccca tctatcgggc
     1561 gaccagaaac tggtcggcag tggtatggta gccgcccagt tgctcagaca cctgttgggc
     1621 gaggaaacgt ttctccagca actcacctat ctactccgta ccggtccacc agatgggcaa
     1681 gacttgctcg gcgcggcgaa tttcgccatc atcgcctccc gcctggctca ggaaggacgc
     1741 tatggtcgca tggcggcttt tgtccaggat caaatgtgga ccgacgttga tctaaacctc
     1801 gctattgagg gcgtgagaac cgtagatgtg gatgcctggt acgacacgaa ggagtatcgg
     1861 ccaaaactga gtattatctg gtcagctgga gaggtataag caatcgacct gggaacggag
     1921 aaagcatatg agtagcgcaa atgggaacaa ccgcttagtc cagatcgttg atctggtaaa
     1981 gagcttcccg gtgggtggcg gggaagtgac tatcctcaaa gggttatcgt tcgatgttaa
     2041 acaaggggag ttcgtctcca tcgttggtcc ctccgggaac ggcaaatcaa cgctgctcaa
     2101 tatgatcacc ggtattgatc gacccaccgc cggacaggtg atcgtcacgg gactggaaat
     2161 caacaagatg agcgagaatc agctggcagc ctggcgtggc aagcacgtcg gtatcatctt
     2221 ccagttcttc caaatgttgc cggcgctcag cctgctgcaa aacgtcatcc tgccgatgga
     2281 tttcgccaag aagtacagcc ctcgcgagcg gcgggagcgc ggcatgcacc tcctggagat
     2341 cgtcgggtta gaggaccagg cgcataagct gcctagcatg gtctccggcg ggcagcagca
     2401 gcgcgccgcc atcgcccgct ctctggcgaa cgaccccgat ctactcgtag ctgacgagcc
     2461 caccggcaac ctggacacgc gcaacgccgg cgacgtcttc gacctgtttc tcaagttggt
     2521 agatcagggt aaaacccttc tcatggtgac gcacgataaa gagctctcac ggcgcgtgcc
     2581 acgcgtggtt gagatcattg atggtaagat cacccgcgac gaatttgtgg gtggtgcagt
     2641 atggaccggg tattaaggat ttagctgatg ggcgtcttgc gttataagat ttaccgcgat
     2701 ctatgggtca atttcggccg taccctgcag gtggtattaa tcatcgccat cggtgcaggt
     2761 gctattggca tgatcgtggg cacgcgctat ctggtcatca gcaacatgca atcagcctgg
     2821 cgcgccatga acccagctac gatcaacctg ttcatcgggc caccaataga cgaggatgac
     2881 ttaatcgctt tagaaagcga agatggtgtg gtcgagatcg aaggtctggg agattccaac
     2941 atcgaatggc gcctcaatcc agacgatgaa tggcaactcg gagggctgac cttccgtgcc
     3001 aattacgagg agcagaagct caatttgttg gaactcgtgg aaggcgattg gcccagcgga
     3061 aaatttgtgt ccatcgggca gggagatgac caattcttcg gcgtccccaa gaatgggaaa
     3121 gtctacctgc gcctggacga aaaagaatac accatccaga ccgatggcgt cgtatataac
     3181 cagcttcagc agccggcgta ttttggcggc acggcccagt tctacgccac ccaggaagaa
     3241 tatgagcgca ttgtcggcga ccgcgatttt agccaggtac tggtgactgg cgtagaatac
     3301 gatgagctca agttggccga actgggagat cgcctgcaag atcgcatcga aaagatgggc
     3361 agcgattcaa gtcgcatgat tctcgatcct aacgagcact tcttccaacc tcagctggac
     3421 ggtatattct taatattggg cgtcatgggg ggattggcat tagccctggg tctcttactg
     3481 gtgtacacca cgatcaacgc catcatcacc cagcaggtgg accagatcgg catcatgaaa
     3541 gctatcgggg cgcgcacctg gcagatcctg cgcttgtacc tgaccagtat cttcttttac
     3601 agtatcctgt cgttgttgat tgccttgccc ctgggattgg gtggtgcttg gtctatcacg
     3661 tcatggttgg ttacaggttt tggcgccgat ccgggctctt tcgatttttc acgtgaagca
     3721 gtaattgtca tgctgttgat cgtattcctg gcgccgtttt tggcatccct gctccccatt
     3781 ttcaacgggg cgcgcatcac tgtgcgcgag gccatcagca cctacggtct aagcactggc
     3841 gccggcttac tcgaacgcct gctggccaaa gccaaagcca tgtcgcgcat gttgctgatg
     3901 gtcatcagca acactttccg caataagtgg cgcgtggtgc tgatgcagat caccctggtg
     3961 ctcagcggat tgatcttcat gatggtggtg agcttgcgcg actcggtggt gtactcggtc
     4021 aaggatatcc tcttctctat cctgaacgcc aatatcactt tcctgtttga agagccgcag
     4081 cgtatccatt acctggaaga tttgaccatg aaataccctg gcgtgaaggc agtcgaaatg
     4141 tggggctttg ctggagtgaa catgcgcccg gcaggtcagg aagcatctga ggacgacgag
     4201 ctcgcttcgg tatggggtgt acccttgccc acccaaacct acggctacca attgcgctcc
     4261 ggtcgctggc tggaacccac cgatacccat gccatcgtca tgaaccagca attggccgat
     4321 gatgtgggtg tgaccgtagg agattggatc accgtcaaat atgatgagaa gaacgaagtg
     4381 aattggcaag ttgttggatt ggtattcgac ccgatcttga ccaacgtcgc acttgcttcc
     4441 cgcgaagtgt tgctacacga ccttcgcttt gtgggtagag cccagtcagc ctggattcaa
     4501 accgagcgcg acgacatgca aacggagatc gcggttgcca aaggtttgcg agaattctac
     4561 aaacagaatg gggtagaggt cagcctgcgt aagggtgtat tcggcatggg aggcgacagc
     4621 acgacccagg ccgccaacgc cctgattgca cagtttaaca catttatcgc actgccaggg
     4681 ctcttagcgg tcatcattgc ggcggtggga ggcattgcat tgagcggcgt gctcacgttg
     4741 agcgtgctgg aacgccgccg ggagattggc gtgatgcgcg ccatcggagc ttcttcgttc
     4801 accatcgcgc gcctgttcat cggcgagggc ttgctgctgg gttggatgag ctggctgatc
     4861 gccctgcctc tcagcatccc ggtggggcgc ctcatgccac aagccgtggg cagcgccctg
     4921 cagcttgatt tagtatataa atacacacct acgggcgctg tgctctggct ggtcattatc
     4981 acatttttgt caattatcgc cagttggctg cccgcgcgag gggctacgcg catcagcgtg
     5041 cgcgagagtc tggcttacca ataaaggaga cacctgggat gaaatctaaa ttgttcactc
     5101 tgatcatcat aatcgctctg ggcagcctgt tcttgtctgc ctgcggctcg attggcgcgc
     5161 cagccgatga cgccgccccg accgaaatcc ccatcgtcat caccgacacc gatgtggtcg
     5221 ccgagggtcg cctggtgccg aatgattcgg ccaacctgtc ctttaaagca gccgggcagg
     5281 tgaccgaaat tctgatcacc gagggagaca ccgtcgaagc tgggcaggtt atcgctcgcc
     5341 tggataacag tgagcagctg caatcggcaa ttgccaacgc agaggcagag ttgctaaacg
     5401 cacagctagc ccgcgacgcc ctgtttgaca atgctaaggt gaactccgcc gcggctctcc
     5461 aggctatcgc cgacgcccgc gacgcggtgc gcgactcaga ccgatacctg aacaacctga
     5521 atgctggcag ccgggacacc gacatcgatc tggccaaggc agatgttgcc atccttaaag
     5581 accagttaga tgaagctcaa gaagactata agccatatga gaacaagccc gaggacgacc
     5641 tcaagcgagc taccttcctg gcccgatttg cccaagctga gcaattatat aaagacgcgg
     5701 tccgcaagct gaacaatctt caaggggagc ccagcaaaat tgaccttgac atcgcggagg
     5761 ccaaccaggc tgttgccgag gcgcaactac tcctcgcctt acaaaattat gatgaaatag
     5821 cttctggtcc agatccagac gacctagccg cggttgaggc gcgcattaaa gccgccgagg
     5881 ctggcttggt atcggcccag gcaaccctaa ataatatcga gctagttgcc ccattcgatg
     5941 gcaagattgt cgatctggta atcaccaccg gtgagcaagt agctccggga gtgacggctg
     6001 ccgtgctggc agatttctct caatggattg tcgaaaccga tgacttgacc gagatcgaag
     6061 tccctgagat aagcataggc cagtccgtta caattgtccc cgacgccttg ccggacctgg
     6121 agctgaccgg catggtcgag cgcatcagcg acgtatttga agagaagcgc ggggatatca
     6181 cctacacggc gcgcatcaag ctcaatgagg agaatgagct cttgcgctgg ggcatgaccg
     6241 tggtggtcac ctttgagaaa tagcgccatt aaccagcaat ttgtacgcat aggcttcttt
     6301 aagcaattgg atcgaaatgt gtctgcagat gaattgtagg caaacgaaaa gcaagaagaa
     6361 aaggacgcta tgtctgtaac tcgaaaaata agtcccctaa tgacaggtgt gagtctcctg
     6421 cttatctttg gctctatcct gctacctgcc tgcagcgcag tggggattgg gccatccagc
     6481 actcctacac cgacagtcac tcccgaggtt gagagcgtta tccaggttag cgccgaaggc
     6541 caagtcgtgc cgcggcatca cgccaacctc tccaccgcgg taagcgggca ggtgaccgag
     6601 cttctcttcc gtgaaggcga gcaggtccaa gaaggtgaca taatcctgcg cctgggcgac
     6661 cgcgagcaat atcaagccga ccaggccgcg gctgaactgg agctgctgag cgccgaacaa
     6721 gcgctcgaag agcttcaaaa gaatgtcggg gtggagctgg cccaaacccg ccaggcatta
     6781 gccctggcaa accaggagcg ttcgaaaatc tttgatgagt accagaactt aagtaagcct
     6841 gtcccccaag atgtgcttga tcaggcttat gccagtgtcc tcatggctga aaagcgatta
     6901 caaaaggctg gcgaagacct ggaaaaaatg gaaaaacgct acaagaataa gagcaacatc
     6961 atgtggcaat tcctgaacaa gaagcagttc aaggatctgc tcgagcagat gcgtagagct
     7021 aggctgtctg cagaggatcg ctattacgat gtgctcgacc gttacaacga tctaaagaac
     7081 cacccagatg agatcgagct cgccaaggcc actgcagacc ttgggcttgt ggatgcccgc
     7141 atcgcagacc ttcagggcga catcgctatg ctagagaaag gtccagactc tgataaagta
     7201 gcctctgcag aagcccgccg caaagcagct caggtcgctc tggaggcggc tcagctggct
     7261 ttgaataaca gcgagattga agcacccttc tctggtcaga tcgcggaaat gaacgtcacg
     7321 caaggcgagt gggtggaagc caaccagccg gtgatcctgc tggcggacct taatcactgg
     7381 gaggtccaga ccgatgacct gacagagctg gatgtgccaa ttgtaagcct ggggcagaca
     7441 gtcaccatta agctagatgc cctgccagag gtggagttaa aaggtaaggt ggatgccatc
     7501 aaagatcttt ctgaagagaa gcgcggggat gtgacgtata cggtgactat cttgttggac
     7561 gaggtggacc cgcgcttgcg ttggggtatg actgccgcgg tcgaattcag cgattaagcc
     7621 aaacattggc atatatttca aaacatggac aataaaccaa caagcccctt agcggggctt
     7681 gttttcttca tcgaagctca caaaatagaa aacccggcta atctctgaaa actatctcgg
     7741 gaagcatccc atccaggatg atctctagcg ccatagtatg gctacagcgg tcgcgcgttt
     7801 ggaagaaatc gcaatcgcat tgccaaacgc cattttccag gctgaccgta tgagggttat
     7861 tctctccttc aaaagtcacg gtgagcgaat cgatgcgtat tcgatcacgt tgctcagcat
     7921 atcgtttcgc tttttctatt ttaccaatca ttccataatc cattcgcgct gtctcctttc
     7981 aaaagaggga taatagaaaa ggcacgcgag aagactcgcg tgcctttcac accaaatatt
     8041 cagttgaaga aacatctcgc atatctcttc tccgtctgca ttgagtataa gcggagtcta
     8101 accgaaagtc aaccttcaat ttaggtattt tctcttaagt tcgtccttaa acaccaggct
     8161 caacacgatt gaaatcacgc tgaccacaat ggagcctaac aaggcagacc agaagttgtc
     8221 aaccgtgaaa cccacaccaa aagatgagcc aatgttacca gccaaataga ataaacccgt
     8281 gttgatgacc agagtgaata atcccagtgt aagcaggatc aacggacagg tcaaaaactt
     8341 gagcagcggg tttagaaagg cattgaccac accaaagatc agcgccagcc agatgaaagc
     8401 cagccagttg gtactctggg gttggattcc aggcaccaat gcgacagcca catacaaagc
     8461 aacggcattg atcccccagc ggatgagaaa acggttcatc ttataatcct ttcttttcac
     8521 taaagagaca accatcttgc cgagaccctg aggatctgag cgatcctcag gatctcggtt
     8581 tgcaggagca ctgcataact atttacgaaa tttggtttgc ttagtcgcga aggatgcgct
     8641 tatccacgat aatgcgcact ttggcttaaa gaaatggtag ggcaagttgg caacttaccc
     8701 taccataacc caaaaggagc accatcgcaa gggtagctta ccctgattta cttctcgaag
     8761 tactccacca gttgctggac gtgcagcacg ccgtcaaact tcagcccatc gccacccttg
     8821 gtgagcacgc ctttctcccc atcggggtcg cccaggctgg tgacgatgat atctccggag
     8881 ctcacatcct ctttctctgt gtagtagttc gtggttacca cagttggcat ccccgccgcc
     8941 ttcgcagcca gcacaccgtt cttcgaatct tcaaccacga agcactcatc cggcttgagt
     9001 ccggtcctgg agagtgccag gttgtagatc tccggatcgg gctttttctt gctcaccaca
     9061 tcgcctgcca gcaccagatc gaacttgatg tcggagagga tttttcccgt gatagcccta
     9121 gcggactgct cgttggaagt tgtgcacacg ccaatcatca gccctgcctc caccacttcc
     9181 ttcataaagc ggtgcacgcc cggtcgcagg gggagttgtc ccgattcaat gagctcgaca
     9241 aataacgctg tcttgcgctt gtgcattgtc tttaccagct catcgatctc gtcgggggga
     9301 acgggtgggt tgaagccctt tgtcagactg tgatgcttca tgcgttcttt gccaccggcg
     9361 atttgcagca gctcgtgata ataattcacg ctccattcgt cggtgaagcc gaactcttta
     9421 aaggtcatat tgaaagagac gcgatgacca tcccgctcgg tgtcgatgat gacaccgtcc
     9481 tggtcaaaaa acacagcttt aatttttccc atgattcgcc tccagttatg atccactcat
     9541 tttcgctaat gaaagtcaag ctttttatga aaatttgcgt atttagcgga ccctcttctg
     9601 agcttaggat tgcgaaaagc tcaggacttg gttaagaatt cgcgcacgat cgcgaccgcc
     9661 atgcggttct cttccttact gccaatggta atgcggaacc aaccatcgct tccgtattta
     9721 tcgctatctg ggcggaagat cagacctttt tgttcggcgt acgccaccat atccttgccc
     9781 cgcttgccgg tccccccagc gtcgaagagg acatagttgg cttgtgagcg gaaaatgacc
     9841 aatccaggga tatcgctcag cgattcctgg ataaaatcca cctcgctgtt attgaatttc
     9901 acccggtact ccaaggcttc ggtatcttcc aaggctgcta gcgcggccca catcggaatg
     9961 gtgcccacat tccatggaag cagcgctgcg gagatttgcc caataacgct cttatctccc
    10021 agggcatagc caaagcgcaa tcccgccagg ccataggctt tggacagcgt gcgcgtaata
    10081 atcacgtttt tatacttctt ggtcagtcct acctgagaca tgcccaaccc ggcatattcg
    10141 atgtaggcct cgtcaacgac aaatgggata cccgtctcag cgatccgcac aaattccttg
    10201 gcatccatga agttgcctgt agggttgttc ggattggcga tgacaatgac tttggtctta
    10261 tcagtgacag ctttgaggat ggaatcggga tcgtagagca gctggttatc cttatatacc
    10321 atgggaacag aaaccagctt gccgcccaga atgttgcagc gcagtttata gattccaaag
    10381 cagggtgtgt gttggatcac ctcttcccca ggagccagaa agacgcggaa gatgttatca
    10441 aacacttcgc tggagccgtt gcccaacatc acgttatccg gcccatccaa gccattgatc
    10501 tcagcgatct taccgcgcac caccaaaccc tggtcggggt agcgatgacc caggcgggca
    10561 tacttatcaa tggtcgccag cactttctcg gatggcgggt tcgggttctc gttagacatc
    10621 atgcgatgca gctccggctg gcgccaggca agctcaatgt gccctgatat gtacatcggg
    10681 atacccttca cccaaggaac ataatactct tcaatacttt tcatgttgga actccttgat
    10741 aattaggttg caagtatagt aaagaatgcg tagtgaggag gttcaatggc tcctcactac
    10801 gaagctttca ttgtttttta tgctttccct gcgctgccga gcatatcgat ccaatgccca
    10861 acagcctggc ggatagcgtc gcggacagct acatcgacct tgccgggatc atattcttcc
    10921 ctatgcgccg tgatatagtc gtaggttgaa ttgatcaggg tgtgcttgag ctccgtggaa
    10981 acgttgaact tggcgccgcc catttcaatt aatttctgga tgtaatcttc cggaagaccg
    11041 gtacccccat gcactaccag cggtgtctta actccaccgc cgttaagcat ctggttgatc
    11101 ttgcccaggc gctcaaaatc caccttggga ttcttggtct tatacacgcc gtgggccgtg
    11161 ccaatggcag gggcaaagat atccacgccc gtgcgctcga caaattcaac cccctggact
    11221 ggatttgcca gggcagcctc gtcttcagcg accttgattt gatcttccac gccgcttacc
    11281 gtacccagct cgccttccac agagatatca ccgaccgagt ggcagtaatc caccacctct
    11341 ttggtttggc ggatgttctc ctcaaaggtc tgcttagatg catcgatcat gatgttcgtg
    11401 taacccgcat cagcgcacgt tttgcagtag tcaacttctg tgcagtggtc cagatgcagg
    11461 cagatcggaa tgggtgctga ctcagcgatc gtcttgtaga tcgccaccag caccttcgtg
    11521 cccaagaatt tggatggttt caccgaggtc tggatgatca atggggactt cttctcaact
    11581 gccgcgtcca ccactccctc caactggatc aggtcggtga tgttgaaggc gccaacggcg
    11641 tatttgccct tcgctgcctc cacaaggatt tcttttgcgt ttacgattga catatacggc
    11701 ctcctatact gcaacgtatc ttttcttttg cccacaaatg cgttgtgggc gtatttgcgt
    11761 tcagcgctgg tatctttccc aatgaagagc ggacccagcg taatgccatc tattttccag
    11821 ctacatccac cagccactta tcccagcgag gatctgtgac atgctggcgc gcgcgcccga
    11881 accacaggtc agcatagcct tggaagtcct catccagttt ggagattccc gtctgggtag
    11941 tgccccacat ggcttcgtga atctccgaca tgggccacat gatcttaagg cgagcaaaat
    12001 tcttcggcgt cacctcgcca aaatactcct gcaagagcaa ccttacctga tcatcgttca
    12061 ggcggtgatg gtggctgaaa ttcgccaagt cgaagaagat gtcacccatg ccggcatatt
    12121 cccagtcgag caagcggatc tcgccgatct caccgggcac atcttcatcc agccagttca
    12181 ggttgagcag atcatcgtgg cagggggtgg ggatgtaggg atctttgagc aatgcaccct
    12241 ccgcttcgcg catcttctgc atgatccaat cgaagtcgaa tgggaacttg cagttgttgc
    12301 gcttagaggt ggcagtgagc atctccacgc ggcggaacac gttaaactcg ttgtccagtt
    12361 gtggcgcgtt cctgtggaac aatcggatct tgcgtaccac gcgcacaatg ttttcaggcc
    12421 ttgtgatcac atcgggaggg atgtgcttcc ctttaacata gcgagtgagc aggtagcctt
    12481 caggctcaat aaaatagaca acctcaggag cgacacccaa tagaccggca gccaagttgg
    12541 cttggtattc gacctcgcgg cggataccca acgcatccgt accttctcca gtgatacgca
    12601 agacgtaagc tttaccgtct gtctcgattt tatagtttag attggtaatt ccaccagata
    12661 gcgatgtgat ttttatatca tttgcctccg ccaggaatgg caccctggcg atagcttgct
    12721 caattgtcaa acccatttaa atcctccatc aataagatca tgccgatcga caaatactct
    12781 accatgacca acctaccaca tcaactgtca acgctcaagt gacaccgtca acgaatagat
    12841 atcgccgcgg ttgtagcagt aggtaaactc cacaggcgtg ccatcatcgg cataaacggt
    12901 gcgttcctta taaagaacag acgcaccttc cctaattccc aataattttg ccagctcttt
    12961 tgtggcttcc ttggcctcga tccgctggat ggcacgtttg actttgtacc cgctctgctc
    13021 aaatagcgac cagagatttt gcacctccag attttcatca atcagttttg agaacaattt
    13081 gtgcgggata tgcgcatcgt ggatagtgat gggcacattg tcggcaaatc gcagggtctt
    13141 aacacggatc acaagctcgc caaccgtaat atccagagcc ttagcaatct tttgtttcgc
    13201 ggggatcacc tctaaatcca gcacggtcgc cttggcatga atatcttcgc tttcggtacg
    13261 atcaaaaaaa ttggtcaggc gtgtatggtc acgttccagg tggggaaagg cgacaaaagt
    13321 tcccacaccg tgccgccggt acaataagcc ttcgtcaatc agctctgcga tgccgtgtct
    13381 taccgtcatg cggctgacac caaattttga ggctaaatcg tgttctgagg gcagtttctc
    13441 gccccgcttc aaaacacccc cggtgatctc cttgcgcagg gcctccctta tccatacata
    13501 catagggatg ccctcaattt tggtcaattc aaacatttac gttctcattt gtcagatatc
    13561 tagacgtata gacatctata ttatacaaat ggagatttat atttgtcaat ggacgcagcc
    13621 gatttcctcc ctacccataa atctctatag agggaagatt cgccgaaaat catgattgcg
    13681 cattcgcaga cacgattttt cagctcgatc tcccctctac cagtgaagaa aaggctagga
    13741 tatgagcagc tttaatgact acgcccggat tcgctcgcct ttcggatccc acagcggcat
    13801 ctgtctgaca gtggcaacaa tctgggcacc gaaaaactcg atctccaact gtgtgccagg
    13861 ctggctgtgc tctataggca aataggcaaa gacgatgctc tcctcaaccg aatagccgta
    13921 gccgcccgag gcgacccaac cgacgatgtt atctgtacct aacaggcgga taggttcctt
    13981 gccaagtgcg atagtgcgct tatcatccag cgttgcacag cacagcttgc gcttcaaccc
    14041 gctttccttc tgttttagca gcgcctcgcg acccaagaag tcacccttgt tcagcttcac
    14101 cgcgaagcct aatccagcct cataggggtt gtaatccggg ctgatctcac cgctccagta
    14161 gcggtaggct ttctctaagc ggagtgtgtc aatagccttg taacctgccg ccaccatgcc
    14221 ttctggctgg ccggcttccc acaatgtatc ccacaggcgc agcccgtact ctatcgggca
    14281 gtaaaactcc cagcctagct cgcccacgta tgtgactcgt gaagccagta cgggcacgtc
    14341 gcccaccgtg atggattgtg ccgtcatata gggaaaagca gcgttagaga catctgcact
    14401 ggtggttttt tgtaagatcg cccgcgcctt gggtccccac attccgatac atgtgtacga
    14461 agaggataca tcttctatcg tcaccgaccc atcctcgggc atatgcaggc gcaaccaaga
    14521 catatcatgc tgaccaaatg cagtaccggt gatgataaag aaacgctctt ccgccagcct
    14581 tgtgacggtg aaatcgcact cgatgccacc gcgcttgttc aggcactcgg tatacaccac
    14641 actgccaact ggcacatcga tctcgttggc gcagacatag ttcaagaagg ctaaagcccc
    14701 tgggccgcgc acctcgaact tattaaatga tgtttcgtca aataagcccg cgttttggcg
    14761 cgtcatcaag tgttcatatc cgatagcgcg tgaccaattg tgccgtgccc agccagttgg
    14821 ctcgtgcccg tggacagcca gctcttcgta gggcttgaag taattggggc gctcccagcc
    14881 agtcttctct ccaaagagtg ctcccagatc gcgcaagcga tagtaagtgg gagagatgcg
    14941 caggttgcgc cgggataggc gctcttcgcc agggtagtgg atatcatagt acatagaata
    15001 ggtctcaatg gtccgcgcga cggtgtagtc ttggctggca taatttgtgc caaagcggcg
    15061 aacatccagc cgccacacat cccattctgg ttgtccatcg ataatccact cagccatcac
    15121 cttaccgatg ccacccgcac cagccaggcc gtgagcgcag aaagcgcatg ccacccagaa
    15181 acccttgacc gaagtcggtc caagcaagaa ctcaccatca ggggtaaacc cctctggacc
    15241 attgagcaat tgaatgatct cagctgtctc caccacggga acacgcttga tagagttctc
    15301 catcaatggc gcaaaacgct cccagtctgg cggcagcaac tggtatttaa aatcgcgcgg
    15361 gatgcctttc agcccgaacg gctctggatt gcgctcataa ccaccagtga cgagcccacc
    15421 cacctcctcg cgccagtaca ccagcaaatc ggggtcgcgc agcgtgggaa attcatggcg
    15481 gacgccctca atcggcttcg tgatgatata cagg
//
LOCUS       DBNGSX010000323         7039 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_442232, whole genome shotgun sequence.
ACCESSION   DBNGSX010000323 DBNGSX010000000
VERSION     DBNGSX010000323.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7039)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7039)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7039
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_442232"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="AC16CB_09725"
     CDS             <1..845
                     /locus_tag="AC16CB_09725"
                     /inference="COORDINATES: protein motif:HMM:NF015715.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HIR3342787.1"
                     /translation="IASPSMDPEIFFLSVAMLGWELATVRLLATLALSIAAGFTTHWV
                     IQRGWLGENILRTQKPPKVLKGRDLLRAGWHFVQEKWTNLLRPNPAPLYLATEAACCN
                     SQSVSTINPTGSQGARLLQIAVPATQGNSTVTQSTPALPQDSFWSRLWVETSKAVLLV
                     VQFMALAFFLEALIELYVPQAWVASLVGRQSAYAILTAALLGVPVYTSNLTALPMISG
                     LLGQGMSPAAALAFLIAGPTTTLPAMSAVWGLVSRRVFALYVAFSLLGAILFGYVYQL
                     LLSL"
     gene            complement(996..1646)
                     /locus_tag="AC16CB_09730"
     CDS             complement(996..1646)
                     /locus_tag="AC16CB_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3342788.1"
                     /translation="MTDKDKIRILIADDHPVFRFGLRALLQAQSEMEVIAEATSGEDV
                     VAQAAELQPDLILMDIHMPGMNGIQATRRILQTTPHIGILVITMFDDDSIFDAVRAGA
                     RGYLLKGADPDETVRAIRAVAHGEAIFSPKVAERLMSFFVHQQPASSSHVFPELTGRE
                     REILGLISQGYTNTAIADRLVISAKTVRNHVSNIYSKLQVADRLQAIIRARDAGLG"
     gene            complement(1643..3559)
                     /locus_tag="AC16CB_09735"
     CDS             complement(1643..3559)
                     /locus_tag="AC16CB_09735"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HIR3342789.1"
                     /translation="MPADLVLAGLEAYGASPATYTWFHDLRILLFAVISIITGIMIYL
                     RKPSEWITVYFSLVLVAMGVLISLELQSPPADLPPIYSLLINSAFTAFFFSFYIFPDG
                     RFVPGWTRWLIPGWVVTIVSVSLFSGTGLDLNSWPPLISVPIYLVLLGSSPAAQVYRF
                     RKASNTDQRQQIKLVVYGLTIFVTSFLGFWIPLMFASELQISGLTAVIYDLFGGTLLI
                     IAFAVVPLSIGAALLRYRLWDIDPIINRTLVYIVLTAMTIAIYVLLVGLLSTVFQMRS
                     HPVFAFLATGVVAVIFQPLRQRLQQVVNQFMYGERDNPYTVITRLGQRLGATLTPDAV
                     LPTIVETVKDALKLPYCAIALQQDSGMEVVASDGVLSGMPVRLPLVYQNQAIGELVLA
                     TRSPGEDFNPADRRLLEDLAGQAGIAAHSVGLITELQHARERLVITREEERRRLRRDL
                     HDGLGPHLASQTLTITAARQLLRQDPEAADQLLAEVVKHAQSAIDDIRRLVYDLRPPA
                     LDDLGLIGALNARAKEFEQSGLQITIHVPEKLPPLPAAVETACYRIALEALTNVVHHA
                     MARHCTVSLNLKGKLCLEIIDDGCGLPVEYRAGVGLNSMRERAEELGGSFMIEPALHC
                     GTRVVVELPLAGDS"
     gene            complement(3915..4703)
                     /locus_tag="AC16CB_09740"
     CDS             complement(3915..4703)
                     /locus_tag="AC16CB_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342790.1"
                     /translation="MTTQEITLKKTFDWRVWVFRIIAGLASLFFLAISAPYLLWPWVI
                     VLPDMPDPESQRWFAAQWGVLGTFFWALPLIFVMFKPRKYINLMQYWVIGVLLISLFW
                     FVVGEKTLESFIILALVLFTYPNLRNLFKFEKSALSKKLAILGILAILGCLPRFLIDI
                     NNTLSLIGGSAEDLMVMDNFTHEIDIFLYITLGVIFVALKHSNWKIMGNIIGAALVYS
                     GIAALFTINQAGSWGLLGGAVALLWGVSYLAIIRLEAQTSKDVA"
     gene            complement(5329..5646)
                     /locus_tag="AC16CB_09745"
     CDS             complement(5329..5646)
                     /locus_tag="AC16CB_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342791.1"
                     /translation="MIVRYWRTITLASGADRYHEYLDKNLIPKYRAVDGLEGLFVLRE
                     VRGGLAHFLLISLWKSHQAILSVMAPKAGDAHQPASVNHSYLAFETRAKNYEVLNMMA
                     RIK"
     gene            complement(5945..>7039)
                     /locus_tag="AC16CB_09750"
     CDS             complement(5945..>7039)
                     /locus_tag="AC16CB_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342792.1"
                     /translation="NFIGGAWDIQWHVAIGRDSLFIPPHLLVIFAFVAGLALVLALII
                     YETTMAATGQEMPHAAKLGPFRAPPAFFGIFFGYTAALLSGVLDELWHEIFGIDATLW
                     SPPHLLIMLATMVVDFSLLLGIATSARRQGYEFKWSSPLFWGLVLIGAYAFEAVNFQM
                     GEAFIVGYRQGGVGLYGLLFPLLVGAFLPLSLLACIRLARRFWVVLLIFALAMLLQYL
                     ATGIAAAGFAILKPVSVIDEYVRLNPESTAAKAREFAGLLGFHGLIGFHQAWTMSLSA
                     IPLVLVSLLELFPWARRRPLLAAPLYSVSMVLFSFLWFEQMPALRGYPISSLHLAQAA
                     LISALVGLVTGNLGLRLARLVDTPNTSSTG"
BASE COUNT         1825 a         1833 c         1833 g         1548 t
ORIGIN      
        1 ggattgcatc tccctccatg gacccggaga tcttcttcct gagcgtggca atgctcgggt
       61 gggagctggc tacagtgcgc ctgctcgcca ccctggctct cagtatagcc gccggattca
      121 ccacacattg ggtcattcaa agaggctggc taggggaaaa tatcctgcgc acacaaaagc
      181 cgcccaaggt gctgaaaggc cgcgatctgc tgcgggccgg ctggcatttt gtgcaagaaa
      241 aatggactaa tctattgcgg cccaatccag cacccctgta tttggccaca gaggctgcct
      301 gctgtaacag ccagagcgtc tctacaatca atccaacggg ctcacaaggt gcaagattgc
      361 tgcagatcgc cgtgcccgca actcagggga attctacagt cacccaaagc acaccggctc
      421 tcccccagga ttcattctgg tctcggctgt gggttgagac gagcaaagcg gttctcctgg
      481 tcgtccagtt tatggcctta gcctttttcc tggaagcgct catcgagtta tatgtcccac
      541 aggcttgggt tgccagcctg gtcgggcgcc agagcgccta tgccatcctg acagctgccc
      601 tgcttggcgt gcccgtctac accagcaacc tgaccgccct gcccatgatc agcggcttgc
      661 tggggcaggg gatgagcccc gcggccgcgc tagcattcct gatcgctggc cctacaacca
      721 ccctgccagc catgtcggcg gtgtgggggc tcgtctccag gagggtcttc gccctgtatg
      781 tagccttctc gctcctgggc gcgatcctgt tcggttatgt gtatcagcta ttgctgagct
      841 tatagatcgg ggatatacgc cacgttcagc aaaaagcttc ttatatccgc caatttccgc
      901 gaatttcgga gtattcgtgg attggcggat ttcaattcgg caggggaaca aacaacgaca
      961 cccctctcaa catagaatta attcttagaa ttgcgttagc ccagcccggc gtcgcgcgcc
     1021 cggatgatgg cctggagacg gtcggcaact tgcaatttac tgtagatatt cgagacgtga
     1081 ttgcgcacag tcttcgcaga gattaccaac cggtcggcaa tggctgtgtt ggtgtagcct
     1141 tgcgagatta aacccaggat ttcacgctcg cgcccggtca attcgggaaa gacatgcgag
     1201 gaggaggcgg gttgctggtg gacgaaaaag ctcatcaggc gctctgccac cttggggctg
     1261 aagatagctt ctccatgggc aacggcgcgg atggcgcgca ccgtctcatc tggatcggcc
     1321 cccttgagca ggtagcctcg cgccccggcg cgcacggcat cgaaaatcga atcgtcgtca
     1381 aacatcgtga tcacgagtat gccaatatgt ggggtggtct gtaatatccg tctggtagct
     1441 tggatgccat tcatacctgg catgtgaata tccatcagga tcagatcggg ttgaagctcc
     1501 gcggcttgag caactacatc ctcgccactg gtcgcttctg cgatcacttc catttcggac
     1561 tgggcttgca ggagcgcgcg caaaccaaaa cggaagacag ggtgatcgtc agcgatcaag
     1621 atacgaatct tgtccttatc tgtcatgaat cacccgccaa aggcagctct acaaccacgc
     1681 gcgtgccgca atgtagagcc ggttcaatca taaatgagcc gccaagctct tcagctctct
     1741 cgcgcatcga attcaacccc actcctgcac gatactcgac tggcagacca cacccatcgt
     1801 cgatgatttc taaacacagt ttgcctttta gattcaggga taccgtgcaa tggcgcgcca
     1861 tagcatggtg gactacattg gtcagagctt ccagagcgat gcgataacag gccgtttcga
     1921 ctgccgccgg caggggtgga agtttttctg ggacgtggat ggtaatttgc aagccgctct
     1981 gctcgaactc tttcgcccgg gcgtttaggg caccgattaa gcccaggtca tccaacgctg
     2041 gcgggcgcag gtcgtagacc aaacgacgaa tatcatcgat agcactttga gcgtgtttga
     2101 cgacctctgc gagcagttgg tcggcggctt cagggtcttg gcgaagcagc tgacgtgcgg
     2161 cggtaatggt gagtgtttga ctggccaggt gtggtccaag cccatcgtgc aggtctctac
     2221 gcaagcgacg gcgttcctcc tcccgggtga tcaccaaacg ctcacgagca tgttgcagct
     2281 cggtaatcaa gcccacagag tgtgcggcaa tcccagcctg gcctgccaaa tcttcaagca
     2341 gacgcctgtc agcagggtta aaatcctctc ccggggaacg cgtagccaag accaattcgc
     2401 ctatagcttg gttctggtac actaggggca accggacggg catgccagac agcacaccat
     2461 cactggcaac cacctccatc ccgctgtctt gttgcagcgc aattgcacag taaggcagct
     2521 taagggcatc tttgaccgtc tctacaatag tgggcaggac agcatcgggg gtcaaggtag
     2581 ctcccaatct ctgcccaaga cgagtgataa ccgtataagg gttatccctc tctccgtaca
     2641 taaactggtt cacaacctgt tgcagccgct ggcgcagagg ctggaatatc actgcgacga
     2701 cgccagttgc cagaaaggca aataccggat ggctgcgcat ttggaaaact gtgcttaaga
     2761 gcccgactaa caaaacatag atggcgatcg tcatggcagt taggacaatg tacacaagag
     2821 tgcggttgat gattggatca atgtcccaca agcggtaacg caacaatgcc gcaccaatcg
     2881 agagtggcac caccgcaaac gcgatgatca gcagcgtccc accgaacagg tcgtaaatga
     2941 cggctgttaa tccggaaatc tgcaattcag aggcaaacat cagagggatc caaaatccca
     3001 aaaaactggt cacgaagatc gtcaagccat aaacgaccag tttaatttgc tggcgctggt
     3061 cggtgttgga tgccttgcga aagcgataaa cttgggcagc cggtgagctt cccagaagca
     3121 ccaggtatat cggcacactg atcaatggag gccagctatt gagatctagc cctgtgccgg
     3181 aaaataatga cacagacaca atcgtcacca cccagcctgg aatcaaccaa cgggtccagc
     3241 ctggcacaaa gcgcccatcg gggaagatat agaagctgaa aaagaatgcc gtgaaggcgc
     3301 tgttgatcaa cagactatat atgggaggga gatcggcagg aggggattgc agttccaatg
     3361 agataagcac ccccatggca actaaaacca aagaaaagta caccgtaatc cattcactgg
     3421 gtttccgcag atatatcatt atgcctgtta tgatggagat cacggcgaag agaagtatcc
     3481 gcagatcatg gaaccaggtg tatgtagcag gggaggcacc ataagcctcc agaccggcaa
     3541 gcacaagatc ggcaggcaaa atagaatgaa attggaggct ctctggctta aaaatctggg
     3601 tcgtgatgaa tatgaacacg ctaagaagtg caatcgtacc gcaaccccaa ataattctca
     3661 acaggataga ttgcagccgt ttgctgaact gccatttgcc gaatgggttc cttatcaatg
     3721 cgcccatggt tgttattata cagggttgta agcttgcaag agcccgtctg cgctatattg
     3781 agacatagtg gcttagtagc ataaccgaat cttgagccac actttaaact tgataaatat
     3841 ggtaagtcaa gcagggagta aactgagcgg ttactccctg ccgtacctgc ttcatacaaa
     3901 ctttagaata tctcctacgc aacgtctttt gatgtttggg cttctagtct gattatggca
     3961 aggtaactga ccccccataa tagcgccaca gcaccgccaa gaagtcccca actgccagct
     4021 tggttgattg tgaacagtgc cgcaatacct gaatatacta acgctgctcc gataatattt
     4081 cccatgattt tccagtttga atgcttaagc gcaacaaata taacacccag tgttatgtat
     4141 aggaagatgt cgatctcatg ggtaaagtta tccatcacca tcaggtcttc tgcacttccc
     4201 ccaatgaggg atagggtatt gttgatatcg atgagaaagc gcggtaagca tcccaatatt
     4261 gccaggatgc ctaaaatcgc tagtttttta ctaagagcgc ttttttcaaa cttgaacaaa
     4321 ttgcgcagat tggggtaggt gaaaagtaca agagccagga tgatgaaaga ttcaagcgtc
     4381 ttttcaccta ccacaaacca aaataagctt atcagcagga ctcctatgac ccagtattgc
     4441 ataagattta tatattttct gggcttgaac atgacaaata ttagtggtaa tgcccagaaa
     4501 aatgttccca gtacgcccca ttgagcagca aaccaacgtt gggattctgg atcgggcata
     4561 tcaggtagga caattaccca gggccacaat agatatggtg cgctgatggc aagaaaaaac
     4621 aaggaagcca atccagctat gatacgaaaa acccatacgc gccaatcaaa ggtctttttg
     4681 agggttattt cttgggtggt catctgtaat ctcctttttg aaagatgttc attcttgggt
     4741 attttcagcg gcaacgttgt tttacagaga tgcctccatg aatgtgggac taagggggca
     4801 atccaaggtc ataattcatg tttattgtct cattctccct ccagtagtag ccttgcggtc
     4861 acttggacgt gatccgaagg acagtgtcca gatgttgcta tccaaaaggg tattgctaca
     4921 aaaaacgtaa cccagccgaa taacgtgcga gctagaatgt aagttccttt ttggggtacg
     4981 cctatcgagg tcatgaccag ccccttttcc tatcaattgg acatgagacg agagttcccg
     5041 ttcaacaatc caaagatagc atgaaccctg tccctttgtc atgaggctgc cgtcatgatt
     5101 tgacacagga caatatgtcc caacttgttg agatcgtgga ttaaactgtc agaagcgatg
     5161 cagttggaga agtgaagata gatttcggtg gagatatttt ggattctgtt gacaaaccat
     5221 ccgtctctta gagagtcgcg ctttcctgga tataccctta cccaagaatg aggttgcaaa
     5281 taccgccgtg aataagcttc gtgttttttg gagattttat ggaactggct acttgatcct
     5341 cgccatcatg ttcagtactt cgtagttttt cgccctggtc tcgaaagcga gataagaatg
     5401 atttacagaa gcgggctgat gagcatcccc tgctttaggg gccataacgc ttaagatagc
     5461 ttgatgggat ttccacaaag aaatcaacag gaaatgcgcc aagccgcctc gaacttcccg
     5521 caagacgaat aacccttcca gaccatcaac cgctcgatac tttgggatca ggttcttgtc
     5581 caggtattca tgatagcgat cagccccaga tgctaatgta atcgtgcgcc aatatcgtac
     5641 aatcattcta gcctcccctg gaaacaaatc aacgagcgtt ccagcctaga cactatgcat
     5701 gttgcgatgc tgtaatactt gttacagttt aaggcacaac gcttacgatg agggggaact
     5761 atttgccccg gaactgcccc aaaaagtgat acatttctcg caacacatac cgcttgaatc
     5821 ccgctctggt agctccgcag gtaaccttcc ccctaccccc tcttcggtgg tgaagggaga
     5881 atcttcacgg ttaacggtag cgctacccct cgttcttggt tatgcagtta aataagatgt
     5941 gtacttaccc agttgaggag gtattagggg tgtcaaccaa gcgcgccagg cgcaagccca
     6001 ggttgccggt taccaacccg accagggcgc tgatcaacgc cgcctgggcc aagtgcaggc
     6061 tgctgatggg gtaaccgcgc aaggcgggca tctgctcgaa ccacaggaag gagaacaata
     6121 ccatgctcac agagtagagc ggggcagcca gcagtggtcg gcggcgcgcc cacggaaaaa
     6181 gctccaacaa ggagaccaac accagcggta tggcggaaag ggacatggtc caagcctggt
     6241 ggaagccgat cagaccgtga aagccgagca gtccagcaaa ctcgcgggct ttggcggcgg
     6301 tcgattctgg attcaggcgc acatactcat cgatgactga gacgggcttt aggatcgcaa
     6361 aaccagccgc ggcgatgccg gtagccaggt attggagcag catagcgaga gcaaagatca
     6421 agagcacaac ccagaaacgg cgcgccaggc ggatacaggc gagcagggaa aggggcagaa
     6481 aagcgcccac cagcagtggg aagagcaagc cgtagaggcc aacaccgcct tgccggtagc
     6541 cgacgatgaa ggcttccccc atctggaagt tgaccgcttc aaaagcataa gcaccgatca
     6601 ggacaagacc ccaaaagagc gggctgctcc atttaaattc atagccctgg cgccgggcgg
     6661 aggtagcgat tcccaacagc aggctgaaat caaccaccat tgtcgccagc ataatcagca
     6721 ggtgcggcgg gctccacaag gtagcatcga tgccgaagat ctcatgccat aattcgtcta
     6781 atacaccaga cagcaaggca gctgtatagc cgaagaaaat accaaagaag gctgggggtg
     6841 cacggaaagg ccccaatttg gcagcgtgcg gcatctcttg cccggtagct gccattgtgg
     6901 tctcatagat aatcagagcc aggacaagcg caaggcctgc gacaaacgcg aagatgacca
     6961 gcaagtgtgg cgggataaac aggctatcgc ggccgatagc gacatgccac tggatatccc
     7021 aggcccctcc aataaagtt
//
LOCUS       DBNGSX010000324         3183 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1897072, whole genome shotgun sequence.
ACCESSION   DBNGSX010000324 DBNGSX010000000
VERSION     DBNGSX010000324.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1897072"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="AC16CB_09755"
     CDS             <1..339
                     /locus_tag="AC16CB_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HIR3342793.1"
                     /translation="YAASALMGVFFSAGWSPCVGPVLGAILTFAINGGSIAHGVSMLS
                     AYSAGLAIPFLLAALGIGWVTTILRRYGKVMRYAEIVMGVILVIVGGMLFFGTFQLLS
                     RFGFFVDLGL"
     gene            344..892
                     /locus_tag="AC16CB_09760"
     CDS             344..892
                     /locus_tag="AC16CB_09760"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR3342794.1"
                     /translation="MSTGKRGRVWAYLGSGLLIWLGVVALAAVVAEYVGSDEGVAIAS
                     LNTRAPDFELENLNGEQIRLSQLRGKPVVLNFWATWCAPCVIEMPIIQRSYEKYPGQF
                     QVLAINADEPHLKVERFVEDIGLTFEVLLDPGGAIQELYQIRGYPTSYFVDAEGIIRN
                     THIGTLNADQLSDYLLEFGVGE"
     gene            889..1905
                     /locus_tag="AC16CB_09765"
     CDS             889..1905
                     /locus_tag="AC16CB_09765"
                     /inference="COORDINATES: protein motif:HMM:NF021384.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HIR3342795.1"
                     /translation="MITVTLYSREDCHLCEQVQEQLVALQAEIPHEMVVIDVDSNPDL
                     RRAYGFEVPVVEVGPYTLRAPIEPQDLRITLMAAQDRHQHLDSLEDPNYQRLVQNNQV
                     WTKSDSFAYWLSNHYLAIFNIFVLIYLGLPFLAPVMVKTGFETPARLIYRVYGTMCHQ
                     WSFRSFFLYGEQSVYPRAEAGVEGLLTYSQATGFGEGNTANDLIKAREFIGNEKVGYK
                     IALCERDVSIWGGILLFGLLYAISGRRFPALPWFLWLLIGILPIGLDGFSQLLSQPPF
                     NFYVYRESTPFLRVLTGGLFGFSTAWFGYPMTEMAMAETRRIMASKFLRIQSRVTPSE
                     VATD"
     gene            1913..2701
                     /gene="pgeF"
                     /locus_tag="AC16CB_09770"
     CDS             1913..2701
                     /gene="pgeF"
                     /locus_tag="AC16CB_09770"
                     /inference="COORDINATES: protein motif:HMM:NF014619.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HIR3342796.1"
                     /translation="MRLNQAEEIRYFTFDHLDNANLVHAVFARRGGVSPYPWESLNVG
                     GLVGDDPSRVLENRRRSFRAVGRKMETMFDVWQVHGDEVVCAESPRPLDVPHQKADAI
                     LTDRVGLTLFMRFADCVPILLYDPYRRVAGLAHAGWMGTVKQTVKAAIVAMQDKYHSK
                     PGDILAAIGPSIGMHHYEVGPEVAKQVSDVFGSDAKTLLVSPNGSEDRVQFDLREANR
                     LILEQSGVRQIEVARVCTACEAQDWYSHRGEKGQTGRFGVLIGL"
     gene            2709..>3183
                     /locus_tag="AC16CB_09775"
     CDS             2709..>3183
                     /locus_tag="AC16CB_09775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR3342797.1"
                     /translation="MASIIQERYEMVLARINRSAQSVGRLADEVCLVVVTKGQPLERV
                     QAVIQSGARHLGENYAEEGVEKLEALSGPLGVTWHMIGHVQSRKARLVSNHYDWVHSL
                     DSLKLAEKLNRFAAESGRRLSILLECNVSGEESKYGFPAWEDERWEDLAEAVTPIL"
BASE COUNT          731 a          677 c          917 g          858 t
ORIGIN      
        1 tatgcagcct cggcgttgat gggcgtattt ttctcggcgg gatggtctcc atgcgttggt
       61 cccgtgctcg gcgcgatatt gaccttcgcg atcaacggtg gttcgattgc gcatggggta
      121 agtatgctat cggcttattc agctggactg gcaatcccat tccttttggc ggcactcggc
      181 attggctggg tgacaaccat cctgcgccgc tatggaaaag tgatgcgtta tgcagagatc
      241 gtcatgggcg tgatcctggt gatcgtcggt ggtatgctgt tttttggaac cttccaatta
      301 ttgtcacgtt ttggattctt cgtggattta ggtttgtaac gagatgtcaa caggcaagcg
      361 tggccgagtg tgggcttatt tgggtagcgg cctgctgatc tggctcggag tggttgcgtt
      421 ggcagctgtt gtcgccgaat atgtaggtag tgatgaagga gtggcaattg cctccctcaa
      481 tactcgcgcc ccagattttg agctggagaa tctaaatggc gagcaaatcc gcctgagcca
      541 gctgcgtggc aagccggtag tactaaattt ttgggctaca tggtgcgctc cttgcgtcat
      601 cgaaatgcca attatccaaa gatcctatga gaaatatcct ggacagttcc aagtcttggc
      661 gatcaatgcc gatgaaccac atttaaaggt agagcgattt gttgaagata tcgggctcac
      721 tttcgaagtg ctgttggatc ctggcggcgc aattcaggaa ctgtatcaga tacgtggcta
      781 tccaacgtca tattttgtcg acgctgaggg catcatccgc aacacgcata ttggtacgct
      841 taatgcggac caactctcgg attatcttct agagtttgga gtaggagaat gattactgtt
      901 acgctttata gtcgagaaga ttgccatctc tgcgagcagg tgcaggaaca attggtggct
      961 ttgcaagcgg agattcctca tgagatggtt gtgattgatg tggatagcaa tccggatttg
     1021 cgccgggcgt atggatttga agtccctgtg gtcgaggtgg gtccctacac gctgcgcgct
     1081 ccgatcgaac ctcaggattt gcgcatcaca ctcatggcgg cgcaagatcg ccatcagcat
     1141 ttggactctt tggaagatcc taactaccag cgactggtac aaaacaacca ggtgtggaca
     1201 aaatctgata gttttgccta ttggttgagc aaccattacc tggcaatatt taatatcttt
     1261 gtcttgatct acttgggatt gccatttttg gccccagtga tggtgaaaac tggctttgaa
     1321 acaccggcga ggctaattta tcgcgtctat ggcaccatgt gtcaccaatg gtcgttccgt
     1381 tccttctttt tatatggcga gcaaagtgtt tatccgcgag ctgaggctgg cgttgaaggg
     1441 ttattgacat attcccaagc cacaggtttt ggcgagggta acacggcgaa tgatctgatc
     1501 aaggcgagag aatttattgg caatgagaaa gtcggttaca aaattgcctt gtgcgagcgt
     1561 gatgtttcaa tatggggtgg tattttatta tttggcttgt tgtatgctat cagtgggcgg
     1621 cgttttccgg ccttaccctg gttcctttgg ttgctgattg gtatcctccc gattggcctt
     1681 gacggtttta gccagttgct cagccagcct ccatttaatt tctatgtata tcgtgagagc
     1741 acccccttcc ttcgcgtgct tactggtggg ttatttggat tctccaccgc ttggttcggt
     1801 tatccaatga ccgagatggc gatggcagaa acccgccgca ttatggcgag caagttcctt
     1861 cggattcaga gtcgagtgac accaagcgaa gttgccacgg attagatgcg atatgcggct
     1921 taaccaggcg gaagagatcc gttatttcac tttcgatcac ttagataatg caaacctagt
     1981 ccacgcagta ttcgccaggc gagggggtgt cagcccgtac ccatgggaat cgctcaatgt
     2041 ggggggctta gtgggggatg acccatctcg tgtccttgaa aatcgacgca ggagtttccg
     2101 agctgttggg cgtaaaatgg agaccatgtt cgatgtctgg caggtacatg gggatgaagt
     2161 ggtgtgtgca gaatctccgc gaccgttaga tgtccctcac caaaaagcgg atgccatact
     2221 gactgatcgg gtgggtttga ccttgttcat gcgatttgca gattgcgtgc caattctctt
     2281 gtatgatccc tatcgtagag tggctggtct agcccatgcc ggttggatgg gaactgtgaa
     2341 acagacggtc aaggcagcca tagttgccat gcaagacaaa tatcactcga aacctgggga
     2401 tatcctggcc gcgattggac catccattgg tatgcaccat tacgaggtgg gaccagaggt
     2461 agccaagcag gtaagtgatg tatttgggtc ggatgcaaaa acactactgg tttcacccaa
     2521 tgggagtgaa gatcgagtac aattcgacct gagggaggca aaccgtttaa tcctagagca
     2581 gagcggcgtg cgacagatcg aggtcgcaag agtgtgtacg gcatgtgaag cacaggattg
     2641 gtactctcac cgtggagaga aagggcaaac tggcaggttt ggggtattga ttggattata
     2701 gggaaggcat ggcatctata atccaagagc gttacgagat ggtattagcg agaattaacc
     2761 ggtcagctca atctgttggc cggttagctg atgaagtgtg ccttgtcgtt gttaccaagg
     2821 gtcagccgtt agaaagagtt caagcggtaa tccaatctgg agcacggcat ttaggagaga
     2881 attacgctga agaaggcgtt gagaagctcg aggcgctttc cggtccgctt ggtgtcacat
     2941 ggcatatgat aggtcatgtg cagagccgca aggcaagact ggttagcaac cattatgatt
     3001 gggtgcattc actggacagt ttgaaactcg cggagaagct aaatcgcttc gcagcggaat
     3061 ctgggcgcag gttatccatc ttgcttgaat gtaatgtcag tggcgaggag agcaagtatg
     3121 ggtttccagc ctgggaagat gagagatggg aggatttagc tgaggctgta actccaattt
     3181 tac
//
LOCUS       DBNGSX010000325        22745 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1273337, whole genome shotgun sequence.
ACCESSION   DBNGSX010000325 DBNGSX010000000
VERSION     DBNGSX010000325.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 22745)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 22745)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22745
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1273337"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="AC16CB_09780"
     CDS             <1..398
                     /locus_tag="AC16CB_09780"
                     /inference="COORDINATES: protein motif:HMM:NF014670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR3342798.1"
                     /translation="GQGFRRFLFLNGHGGNDTARVRLYELANQLPGMHLAWYAWWASH
                     SVEIIAQKYGLKPSHGNWLEAFTFTQVAELPDEEKVPPAVRGLLSAEEARHVYGDGSF
                     GGPYKADKAILDEVFAACLSDILHMLKFE"
     gene            complement(400..1329)
                     /gene="miaA"
                     /locus_tag="AC16CB_09785"
     CDS             complement(400..1329)
                     /gene="miaA"
                     /locus_tag="AC16CB_09785"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR3342799.1"
                     /translation="MSGEFKLPLVVILGPTAVGKTEIAIQLAERLEGEIVSADSRLFY
                     RGMDIGTAKPTPQQRKQVPHHLIDVANPDQPWSLAIFQQQAHLAISIIHSKRRLPFLV
                     GGTGQYIRAVIEGWQVPKAEPRPEMRQTLERWAAQITPLGLHHRLAILDPEAAARIEP
                     RNVRRTVRALEVILSTGRRFSEQRQRSSTPYRTIILGLMRSRTELYACVDERIQAMLA
                     SGLVEEVQTLLERGYSPNLPTLSAIGYREIISYLQGNTTLEEAVAQIKRRTRIFVRRQ
                     ANWFRSNDPNIVWFDIKLGIVDEIELAVRAWLG"
     gene            complement(1329..1697)
                     /locus_tag="AC16CB_09790"
     CDS             complement(1329..1697)
                     /locus_tag="AC16CB_09790"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR3342800.1"
                     /translation="MTSRLSLGRWGEVIAAEYLSSLGYVIIEHNARTPYGELDLIAKQ
                     GDGLVFVEVKTRASLSLGLPEISVTPTKQAHLLSSAQAYLQAHPELGEDWRIDVIAIL
                     RLPGKSPQIEHFENIITSEA"
     gene            complement(1697..2887)
                     /locus_tag="AC16CB_09795"
     CDS             complement(1697..2887)
                     /locus_tag="AC16CB_09795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3342801.1"
                     /translation="MMADLYKPHVSGVTNYIALNKAYLEENGHEVFVFTFGDENIKDD
                     EANIIRSPGLPLLDTGFYINLSYNRDARKLLYSMDVVHVHHPFLSGSLALRYCRPRGI
                     PIVYTNHTRYDLYAQAYVPILGDLISETTMTTYLPVFCRLCDLVIAPSPGLRDVLMRF
                     GIDSPIAVVPNGVDLQPFRNPPRPMERSEFGFGPNDIVLIYVGRLGPEKNLIFLLRSF
                     AGAAKAYDNIALLLVGDGPERDNLEDRAQHMGIDDRVFFTGMIPYEKLPSYLSMADAF
                     VTASITEVHPLSVIEAMAAGLPVLGIKSPGIGDTVENGVTGLLTEEEELAMFTAKMVR
                     LVTEHQQRKEMGQRARQAAENYAIERTNMMMQEKYRFVYEKAGGRRSGWRVRFLRVLD
                     RWGS"
     gene            3152..3961
                     /locus_tag="AC16CB_09800"
     CDS             3152..3961
                     /locus_tag="AC16CB_09800"
                     /inference="COORDINATES: protein motif:HMM:NF040134.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5656 family protein"
                     /protein_id="HIR3342802.1"
                     /translation="MLQRHLPESDRLSILAATILLAYALASFVDLPPREISVQLPGLY
                     LAVELNARTVAALLVAGLTASGADWLLRTHPSVQGHKTFEHWLVPALTALVIGVPLSQ
                     ISGAPQWWLGFIAGGALLMLVLVAEYITVDSDDMRQPIAAAGLTTVSFALYLILAIAL
                     RYAGLRLFLLLPPLILAAWLVSLRTLHLRLHGRWVFLQAGVVALICGQLAAVLHYLPL
                     SPISFGLFLIGPAYALTSLLANLAEDEPLRQAILEPLIVLAILLIITLWIG"
     gene            3970..4377
                     /locus_tag="AC16CB_09805"
     CDS             3970..4377
                     /locus_tag="AC16CB_09805"
                     /inference="COORDINATES: protein motif:HMM:NF025819.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HIR3342803.1"
                     /translation="MIEKLVMTREHWAQILTEVERVVPEEACGLLAGIDERVIESIPV
                     TNTLHSRVRFRMEPLEQLKAFQRIEAQGWDLIGIYHSHPLGQDGPSQTDIDEAYYPEV
                     VSVIVSGRDGDWRCRGFHIWDGQTHEVPVLIED"
     gene            4460..5161
                     /locus_tag="AC16CB_09810"
     CDS             4460..5161
                     /locus_tag="AC16CB_09810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR3342804.1"
                     /translation="MSFVLSVAVIICAYLIGSIPSGLLIVKLTKGVDIRQIESGRTGG
                     TNAMRAGGFWAGLGTAIFDMLKATVCVWLARGLIPGAIWLEIIAPVMAILGHNYSIFL
                     LERGEGGIQFRGGAGGAPAAGGALGLWLPSMFFIVPLAAIILYFIGYASVATLSIPLV
                     TIVIFAIRAWIGAAPWEYVLYGLIAEVLLLWALRPNLRRLFNGTERLVGYRAKRKRKN
                     TDYSTSSSSTPSGSS"
     gene            complement(5119..6024)
                     /locus_tag="AC16CB_09815"
     CDS             complement(5119..6024)
                     /locus_tag="AC16CB_09815"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3342805.1"
                     /translation="MIQRERVADNVYSFQSDLYAQVTAGAVIGPSWAIVIDTLALPEE
                     TLAIREFIEQELGVPIRYVINTHYHADHAWGNYFFPGATVIAHALCRKWLEERGAPSI
                     NEMHKQNLAFRNIKIILPHIIFDEGQLGLRVGKKTLSLMPMPGHCADNIAILVEEDRV
                     LFAGDACMPLPYIVDGDIDDLTASLKKVARMGLENIVQGHGDIILRGEVDSYIKDNLS
                     YLSAIRKAVRKASRRKYPMDMLDEVSIEDCGKSRVLIGGLAVELHRRNLRALYRQIFG
                     VLPQSSMDDEEDYDDPDGVEDEEVE"
     gene            6586..9381
                     /locus_tag="AC16CB_09820"
     CDS             6586..9381
                     /locus_tag="AC16CB_09820"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIR3342806.1"
                     /translation="MPEFSLPKTYDFKAVEQRIYEWWDRMGYFKPSNDPKKPGFDSSK
                     KPYVISIPPPNVTGELHLGHSMFVSMEDLMIRYHRMKGISTLWVPGSDHAGIATQLQV
                     EKSLAKEGLTREQIGREEFLRRTWLWKDKFGGIITGQIRRLGASCDWERERFTLDEGL
                     SRAVREAFVRLYEKGLIYRGPRLINWSPGLKTAVSDLEVEYSEEPGTLYFFKYILEDN
                     EKEYLPVATTRPETILGDTAVAVHPQDSRYQRFIGRNVIVPILGRAIPVIADEYVDRE
                     FGGGALKVTPGHDPNDYAIAERHGLPIISVLDEAGRINENGGPYRGQDRFEGRKNLWD
                     DMRSAGLVIKEQPYTLNVPRTQRGGEIVEPMISTQWFVHIQPLADAALQAVRDGRIRI
                     VPERFTKVYYNWLENIQDWCISRQLWWGHRIPVWYCADCGEMTVTRIDPESCAHCLSP
                     RIEQDPDVLDTWFSSGLWPFSTLGWPDETPDYKYFYPTTMMETGYDILFFWVARMIMM
                     GLEFTGEIPFSTVYLHGLIRDEVGRKMSKTYGNVIDPLTVMDELGTDALRFTLLVGST
                     PGNDMNLSIKRVEANRNFANKIWNVGRFVISSIQEFESDNRWNAEREKSSSPIPYPSH
                     TWTVADRWIYARLAALISDVERLFQNYQYGEAGRQIYEFFWNEFADWYIEIAKLQLAS
                     KAGDGSGAVDREGAHVSPAANYTKSITAHMLVYVLDTCLRLLHPFTPFVTEELWGHLK
                     RALGQTSLSYQMSSEALIVARWPEPPTREPGEKEAVEQFSLIQDVVRAIRNLRAEKSV
                     KPGKRIPALINAGDRAQVLRAQAEAIASLAQLDEQALNIQESIASREEGTIALVVSGI
                     EVYLPLAGLVNLDEERSRLEKELADMERQRKRVQELLSSPFAQKAPPDIVQKERDKLA
                     VLDETVQKLREQLKSIS"
     gene            9455..10252
                     /locus_tag="AC16CB_09825"
     CDS             9455..10252
                     /locus_tag="AC16CB_09825"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3342807.1"
                     /translation="MRVRLKDVYLAVEERGDGRPLLFIHGYPLRGAMWAPQIEGLADV
                     ARVIVPDLRGHGESDATTGAYSMDLLAEDCREMLDAMGIIEPIVICGLSMGGYVTFAF
                     YRKYPERVAGLILAATRAVDDSPEGKANRDKAADLARQGGTGAIVESMLPKMFAPKTY
                     LERADLVQNLREIMQGVSLDGILGDLMGMKSRPDSTPTLGQIDKPTLILHGSDDQLIP
                     VSDASSMQSVVAGSVLRVLQDSGHLLNLEQPDLFNQAIREYLKELHR"
     gene            10249..11121
                     /locus_tag="AC16CB_09830"
     CDS             10249..11121
                     /locus_tag="AC16CB_09830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR3342808.1"
                     /translation="MKFGVIYPQTEYGSDPSAIKDFAQTVEDLGYAHIGCFDHVLGVN
                     PDRPGGWQGQYTYQSAFQEPFTLFSFFAAVTQRTLFTTGVLILPQRQTALVAKQAATL
                     DVLSEGRLRLGVGLGWNAVEYTCLNENFHNRGRRLEEQIEVLRLLWTEPLASFSGRWH
                     TIPDAGINPLPVQRPIPIWFGGRAEPALKRAARLGDGWVAPYRTPTEAMPNFEKFAQY
                     LDEVDRSWEKYSVEVRLYYGPGDPDIWKKLVREWQVVGVDNISFNTMGSVFNSPEEHL
                     AAIRQFAEEMGLHE"
     gene            11114..11596
                     /locus_tag="AC16CB_09835"
     CDS             11114..11596
                     /locus_tag="AC16CB_09835"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HIR3342809.1"
                     /translation="MNKKEIIAAMQNSHELLVEMLNELNTEEMDVPGVVGNWTIKDIL
                     AHLTRWEAELVKLLWQLRQGTQPTTILNQDISLDEVNARWHEEDKDRPLDRILKDFHG
                     VREQTIRRLDHFKDDDLTNPQRYTWLGGRPLWMWVAEDSFEHDLEHMEQIQAWRENRG
                     "
     gene            11902..12285
                     /locus_tag="AC16CB_09840"
     CDS             11902..12285
                     /locus_tag="AC16CB_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3342810.1"
                     /translation="MSQKEVIRTNNAPKAIGPYSVANRIGNLVFTAGQIGLDPTTNEL
                     VSGGIEAETRQALTNLKNVLDAAGSSLEQVVKTTVFLRDMGEFAQMNAIYAEFFPQNH
                     PARSTVQVAALPKYAAVEIEVVATI"
     gene            12463..13455
                     /locus_tag="AC16CB_09845"
     CDS             12463..13455
                     /locus_tag="AC16CB_09845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3342811.1"
                     /translation="MPTVTIIVPCYNEQATIGLLLQAIYDQSYPRVQMEVVIADGLST
                     DQTLSQIQAFTQTHPDLKVRVVENPQRTIPSGLNQALRSALGDIIIRLDAHSIPYSNY
                     VAGCVAALQNQCGENVGGVWEILPGGSGWLAKSIAAAAAHPFGAGDARYRIGGLAQEV
                     DTVPFGAFRRTLIEKIGYFDESLLTNEDYEFNVRIQQSGGKIWMDPQIRTRYFARANL
                     SALWKQYWRYGFWKVRMLRRYPQSLRWRQALPPLFVFSLILFGIFSFWMPFFRWLLIF
                     QLIIYSLVIILAGVRKALSKRYMPYVLGLPLALSVMHLAWGSGALLSMLRYEKP"
     gene            13442..14878
                     /locus_tag="AC16CB_09850"
     CDS             13442..14878
                     /locus_tag="AC16CB_09850"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR3342812.1"
                     /translation="MKSPNYLQKSHPWRLRLQERRTLLLMGDLIIAIISLVLALYFWG
                     IADRFPGWSLEFIQRRVPGWFFLFPIFWLILLVELYDVHRAGNWRATISGVATATLIG
                     FGLYLLLYFSYLDPPKSLLPRRGVAGFFIAAPLLTLIWRLFYIRIFTDPQFMRRVLLI
                     GGGKAGETMLKIINDLWPPPFYVAGIIDDNVAKVGTTIENQQVLGTSESLLELIEHHN
                     VSDLIVAISGEMKSGMFQAMLEAQERGVEIIRMPKAYEDMLGRVPIRLLEADWILRSF
                     VDEHHVSGFYEMVKRIIDIIGGLVGVVMLILLMPFAAVAIWLDDGLPIFYTQFRAGKG
                     SQPYKILKLRTMRRDAEAAGVPQWAREDDARATRVGRFLRKSHLDELPQFINVVRGEM
                     SLVGPRSERPELMGVFQQSVPFYRARLLVKPGITGWAQVNFGYASDIEETTIKLEYDL
                     YYIKHRGLLLDLIIMLRTPATVFGFRGR"
     gene            14880..16841
                     /locus_tag="AC16CB_09855"
     CDS             14880..16841
                     /locus_tag="AC16CB_09855"
                     /inference="COORDINATES: protein motif:HMM:NF014740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HIR3342813.1"
                     /translation="MADQTQSPNGSRRWFAKIWSPWVRARIGSRWLLLIGDLLLIGVA
                     VMGSFGLRLEFGSRFIFFYPQALRMMLLAMLIKPLVYYIFGMYRMMWMYASVKEIRLA
                     ILSVSTGSVLLSIAVIVMTSIQSRLPGYLGFPRAVLVIDWLLSLILVGGLRFSLRILA
                     ELGSGPIEVKGHVRHVLIVGAGDAGALVVREMQKNPQLNFKPVCFLDDDPGKQKQQIH
                     GVSVVGRLDELARVISVRHVDEVVIAIPSAPGSVIRQVADVCRLKSLPFRTMPGIFEL
                     IGGTVSVSRLREVEITDLLRREPTKIDEKIIGSSLGGRRVMVTGAGGSIGRELCIQTA
                     RWGPSELILLGHGENSIFETLIEIEENFPSIPIHPVIADIRDLDRLSVVFDDLRPQVI
                     FHAAAHKHVPLMEANVEEAVSNNVLGTRNIVEVSQNFGIERLVMISTDKAIRPTSVMG
                     ATKRLAELIVLDAAIRTHQAFSVVRFGNVLGSRGSVIPKFKRQIAKGGPVTITHPDME
                     RYFMTIPEAVHLVLQASSMGEGGEVFVLNMGQQVRILDLAEDLVRLSGLEPYKDIDII
                     FTGIRPGEKLSEDLWDQWVQFEPTSHPDILLLNDEDIISGQELRFKVNDLLHLAREGD
                     STAIISILDEFIPGAAVRSTPPPDMTSVV"
     gene            16942..17949
                     /locus_tag="AC16CB_09860"
     CDS             16942..17949
                     /locus_tag="AC16CB_09860"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HIR3342814.1"
                     /translation="MPEVSASEWDKFLSSYKDVHILQTSNWGELKKAFGWQVVRFVCT
                     APSQCGAQILFRRLPFGISLAYIPKGPVGKEWSALWSEIDSVCLQRKAIFLKVEPDLW
                     DGQALDNVQGDAPTGFQVGVQTIQPPRTLVVNLDGDEAQVMARMKQKTRYNIRLSHKK
                     GVVVRPSADLQLFSTLMRITAERDRFGAHSQAYYEKVYDLFHPRGECELLLAEYEGEP
                     LGALMVLASGKRAWYFYGASANSHRERMPSYLLQWEAIRWARARGCLEYDLWGVPDAS
                     EEVLEANFMARSEGLWGVYRFKRGFGGQLRRSIGPWDRVYSSVMYNLYSRVAGARVGF
                     G"
     gene            17968..19158
                     /locus_tag="AC16CB_09865"
     CDS             17968..19158
                     /locus_tag="AC16CB_09865"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HIR3342815.1"
                     /translation="MDHSIVNGAGWNTLIASFPDPHLLQTWEWAQVKSRFGWNPLYMV
                     WKHPGEAGAVIRDLTASTLGRQTPFAAALVLQRNIPIRILSANLRVLYVPKGPLMQDW
                     GNSSHRDRVFHDLKELGRKQGAIFLKIDPDIRLGKGIPGEEGAQEFELGNQISNSLRH
                     QGWVFSSEQVQFRNTLLVDLKPSEELLLAGMKQKTRYNVRLAMRKGVSVRIGTQDDLG
                     LLYRMYAETSVRDGFVIRDESYYRFLWQTFMSGDRSPDISDGPRALPLIAEVSGNPVA
                     ALVLFYFAKRAWYMFGMSREAHREKMPNHLLQWEAMRRAKLAGCTTYDLWGAPDEFVQ
                     SDPLWGVYRFKEGLGGVVVRHLGAWDLPIRPVIYKLYTELLPRILAIMRRRGRSRTQR
                     MVSI"
     gene            19177..20169
                     /locus_tag="AC16CB_09870"
     CDS             19177..20169
                     /locus_tag="AC16CB_09870"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HIR3342816.1"
                     /translation="MINLPRVKIAYLPTPAHAMPRLSAALGGPRLLIKRDDQTGLAFG
                     GNKTRKLELLMAEAQANGAKTLVTAGAVQSNHCRQTAAAAARFGMDCILVLAGEKPIE
                     PSGNLLLDHLFGAELIWTQRDQRDRRLQEVFQNAWEEGRRPYLVPYGGSSPTGAAAYA
                     LAIEELIEQCQTIELEGHSPDWIVFPSSSGGTQAGLVAGARLFGYGGKILGISVDEPA
                     DRLRQNVAELAGRTAEHLGEQISFVPDEILVDDSFIGDGYGIMSEKEREAIHLFARSE
                     GLLLDPVYTGRAAAGLIDLIRRGFFQSDETVLFWHTGGTPALFADRYRNLVENL"
     gene            complement(20166..21887)
                     /locus_tag="AC16CB_09875"
     CDS             complement(20166..21887)
                     /locus_tag="AC16CB_09875"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3342817.1"
                     /translation="MDSETSLAHILNQLITRLSHLTEASRQSINQDPTSLPTQIFDEF
                     GQLKNNLSKVSQHVALLEKHHKDLLSLINIGQVINSSLDPDEVLMTVMDTIIRLLKAE
                     RGFLMLKDTQGKLSIRVARNWTQETVQLSETAISRTVIKRVISDRKPVLTTNAQEDPR
                     FGGQESVIAYHLRSILCIPLKVKDELTGVIYADNRIHSGVFTEYELDLLSAFANQAAV
                     AIENARLFESLRQTLSEVTELKNLMDNVFASIASGVITTDIQEQITLCNHAAEQILGQ
                     TQDSMIGRNLKDILPDQIQGLEMYIERVRQADENIVGIEIDSELSEGKEISLSFNLSP
                     LKDASKTTQGVAIVLDDLTEKKRLQAQRRLFERMVSPAVIDQLDPDKLQLGGKRTDIT
                     TLFVDIRDFTGFSERQSPEQLVGVLNRYLAAAADAVLAHQGTIDKFMGDAVMAWFNAP
                     IPQQDHAIQAVLTALDIREAVQLLQWELPRELHLSFGAGIHYGEAILGLIGTEKRIEY
                     TAIGDSVNTAKRIQENADAGQILISAQVYDLVADQFNIMPARSLAVKGKRKPIAVYEV
                     LGIKRKP"
     gene            complement(22074..>22745)
                     /locus_tag="AC16CB_09880"
     CDS             complement(22074..>22745)
                     /locus_tag="AC16CB_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342818.1"
                     /translation="WPEVYFPEIGWVEFEPTVSQPELSRPLTALGQDNLNNLSSSNLD
                     REDPEPEPPTPRNTLDNPFERTNIWQERLYIIIIAGLFAIMIALLIPLIRRKQLHKRI
                     PFLSIALEKALLRAGLKPPAILSQIALMASLSPLERAYQQINLALVRLGKRPQPTHTP
                     SERTTLLVNRLPSTREPAHLVLTEYQNTIYSQHGLTNQIIAQRAGQEIRRISLRAYIR
                     RRFGV"
BASE COUNT         5467 a         5106 c         6096 g         6076 t
ORIGIN      
        1 acgggcaagg ttttcggcga ttcctattcc tcaatgggca cggcggcaat gacaccgctc
       61 gcgttcgtct ttacgagctg gcgaaccagt tgcctggtat gcacctggct tggtatgcct
      121 ggtgggcttc acatagtgtt gagataattg cacaaaaata tggattgaag ccatcccatg
      181 gaaactggtt ggaggcattt acctttacgc aggtggcaga gttgccagac gaagagaagg
      241 taccccctgc tgttcgcgga ttgttgagtg ccgaggaagc acgacatgta tacggagatg
      301 gttctttcgg gggaccttat aaggcagata aggcgattct ggatgaggta ttcgctgctt
      361 gtttaagcga tatattgcat atgctgaagt ttgaatgatc taacctaacc atgctcggac
      421 agccaattct atctcgtcta caatgccaag cttgatatcg aaccagacaa tattcggatc
      481 gtttgatctg aaccagtttg cttggcggcg cacaaatatt cgtgtacggc gttttatctg
      541 ggcaacagct tcctcgaggg tggtattgcc ttgcaaataa gagataattt cccggtaacc
      601 gattgcagat aaggttggca ggttgggaga atagccacgt tccagtaggg tttgaacctc
      661 ttccaccaga ccggaagcca gcatggcttg gatacgctcg tcaacacacg cgtataactc
      721 agtacgcgac cgcatcagtc ccagtatgat tgtccggtat ggagtggagg atcgctggcg
      781 ctgttccgag aagcgcctac cggtactcag gatcacttcc agagcgcgca ctgtgcgcct
      841 tacattgcgc ggttcaatgc gcgccgctgc ctctggatca agaattgcca gacggtggtg
      901 aaggccgaga ggtgtaattt gtgccgccca acgctccagt gtttgccgca tctcgggacg
      961 cggctcagct ttgggcacct gccagccttc aatgacggcg cggatatact gcccagtgcc
     1021 acctaccaga aatggtagcc ggcgctttga atgaattatt gaaatcgcca ggtgcgcctg
     1081 ctgctggaaa attgccaggc tccaaggttg gtctggattg gccacatcga tcaggtgatg
     1141 gggcacctgc ttgcgttgtt gtggtgtggg ttttgcggtg ccaatatcca tcccccgata
     1201 aaacaaacgc gaatccgctg atacaatctc accctctaac cgttcggcga gctggatagc
     1261 gatctcggtt ttacctactg cagtgggacc gaggatgacg accaaaggga gcttgaattc
     1321 acctgacatc aggcttcgct ggtgatgata ttttcgaaat gttcgatttg ggggcttttt
     1381 ccgggcaaac gcaagatagc gatgacatcg atgcgccaat cttcccccaa ttcagggtgc
     1441 gcctgcaggt atgcctgggc agacgaaagc aggtgagctt gcttggtggg tgtaactgag
     1501 atctcgggta gcccgagcga taaggaggcg cgtgtcttca cctccacgaa gacgagccca
     1561 tcaccctgtt tggcgatcag gtcaagctca ccatatggag tgcgggcatt gtgttcgatg
     1621 attacgtacc ccagggaaga gagatattcc gctgcgatca cctcgcccca gcggcctaac
     1681 gataggcggg acgtcattag ctgccccagc gatccagaac ccgcaggaat cggactcgcc
     1741 atccactcct tcgtcctcct gctttttcat atacaaaccg atatttctct tgcatcatca
     1801 tatttgtccg ttcgatggcg taattctcag ctgcctgacg ggctcgttgt cccatttctt
     1861 tgcgctgctg atgttccgtc accaaccgga ccatctttgc ggtgaacata gccagttctt
     1921 cttcctcggt cagcaaacct gtaacgccat tttcaacagt gtcgccgatg cctggagatt
     1981 tgatgcccag tacgggtaac ccggcagcca tggcttctat gacagaaagt gggtgtactt
     2041 cggttattga agcggtgaca aaagcgtctg ccatagaaag atagctgggc agtttctcgt
     2101 aaggtatcat tcctgtgaaa aagacccgat cgtcgatgcc catatgttga gcgcggtctt
     2161 ccaaattgtc tcgttctggg ccatcaccaa cgagcaatag ggcgatgttg tcatatgctt
     2221 tggcggcgcc ggcaaacgat ctcagcaaaa agatcaggtt tttttccggt ccaaggcgac
     2281 ctacgtaaat taacacaata tcgttggggc caaaaccgaa ttcggaccgc tccatcggac
     2341 gaggaggatt tcgaaatggc tgaaggtcta ccccgtttgg tactacagcg ataggcgagt
     2401 cgatcccaaa tcgcatcagc acgtcgcgca gacctggtga gggagcgatg accaaatcgc
     2461 atagacggca gaaaacggga agataggtcg tcatggtggt ctcgctgatc aagtcgccca
     2521 atatgggcac atatgcctgg gcgtacaaat cataacgggt atggttagtg taaacaatcg
     2581 ggatgccacg cgggcggcaa tagcgtaagg ctagggaacc gcttaaaaag ggatgatgga
     2641 cgtgtacgac atccatggag tagagcagtt ttctggcatc gcgattgtag ctcaggttga
     2701 tataaaatcc tgtatcgagg agaggtagcc ctggagagcg gataatgttc gcctcatcat
     2761 ccttgatgtt ttcatcacca aatgtgaaaa cgaatacctc gtgtccattc tcttctagat
     2821 aggctttatt cagtgcgatg taattagtga caccgctgac gtgtggcttg tacagatccg
     2881 ccatcattcc aattcgcata gtcgaccttt taacgtccgt ttctaatatg ataacctttg
     2941 accattatac tctgggattg atagagcaga gatattggca cggcagatca tctatactgc
     3001 caactattta aaatatagtc ttgggataag taattgtgga aagatgcgca ttgcagggtt
     3061 gttgtatttt cacgcgattc ttttcctctt tgaatgaaga agtcctaaaa aatcaccgca
     3121 attttgcagg ttgcggtaca atccagacgc agtgttacaa cgccatctcc ctgaatctga
     3181 ccgtcttagc atcttagcag ccacgatatt gcttgcgtac gcactggcga gttttgtcga
     3241 cttaccaccc cgcgagatat ctgtgcagtt gccgggttta tatttggctg ttgagctcaa
     3301 cgctcgaacc gtggcagccc tgctggttgc gggcttaacc gcaagtggag cggattggct
     3361 cttacgaaca cacccttctg tgcaagggca caagacgttt gagcattggc tggttccggc
     3421 tttgacagca ttggtgatcg gggtgccact atcccagatc tcaggcgcac cccagtggtg
     3481 gttaggtttt atcgccggag gtgctctgct tatgttggtt ttggtcgcag agtacatcac
     3541 tgttgactca gacgacatgc gccagccaat cgcagctgca ggattaacca cggtctcctt
     3601 cgcgttgtac ctgattctgg ctatcgcact gcgttatgcg ggtttgcgcc tgtttttact
     3661 cttaccccct ctcatactgg cagcttggct ggttagtttg cggacattgc atttacggtt
     3721 acatggccgc tgggtatttt tacaagcggg ggtggtcgcg ctgatctgtg gacagctagc
     3781 tgctgtgttg cattaccttc ccttgtcgcc aatatctttt gggttatttt taatcggacc
     3841 cgcctatgca ttgaccagtc tgctcgccaa tctggctgag gatgaaccgc tgcgccaggc
     3901 gatcttagag cctcttattg ttctggccat tcttttgata atcactttgt ggattggtta
     3961 gcttagccca tgattgaaaa gttggtgatg acgcgcgagc actgggcgca aattttaacc
     4021 gaggtagagc gagttgtgcc tgaagaagct tgcgggttat tagctggaat tgatgaaagg
     4081 gtgatagagt ccataccggt aaccaacaca ttacatagcc gagtgcgttt ccgcatggaa
     4141 cccttggagc agttgaaagc ttttcaaaga atcgaagcac aggggtggga tttgattggc
     4201 atctatcact cccatccatt gggacaggat ggaccctcgc aaaccgatat tgatgaagct
     4261 tattaccccg aggtggtttc cgtgattgtg tcaggacgag acggagattg gcgttgccgg
     4321 ggatttcata tttgggatgg ccaaacccac gaagtgccgg tcttgatcga agattaatct
     4381 tgttctgaga gtaactctgc gctagaaatt gtgttatcct ttacgctgaa atagtattgc
     4441 ctaggaggag ccgtttaata tgtcgttcgt attatcggtt gctgtgatca tttgcgccta
     4501 tttaattgga tcaattccaa gcggcttgct gattgtgaaa ttaaccaagg gtgttgatat
     4561 acgccagatt gaaagtgggc gcactggcgg aacgaatgcg atgcgggcag gcgggttttg
     4621 ggctggtttg gggacagcca tcttcgatat gcttaaggct acggtatgtg tgtggttggc
     4681 gcggggactc ataccgggag cgatatggct tgaaataatt gcccctgtaa tggcgatctt
     4741 gggacacaat tattcaatat ttttgctaga acgtggagag ggtggtattc aatttcgtgg
     4801 aggtgctggg ggcgcacctg ctgcaggtgg agcgttgggt ctttggctcc ccagtatgtt
     4861 ttttatagtt cccttggcag cgataattct atattttatt ggttatgcct ccgtggctac
     4921 cttgagtata cctttggtga ccatcgtgat atttgccatt cgagcgtgga ttggtgcagc
     4981 cccatgggaa tatgtactat atggcttgat agcagaggtg ctattgcttt gggcgctacg
     5041 gccaaatttg cggcgccttt ttaacggtac cgagaggctg gttgggtatc gggcaaaacg
     5101 gaagagaaaa aatacagatt attctacttc ttcatcttca accccatcgg gatcatcgta
     5161 atcttcttca tcatccatag agctctgggg gagcacgcca aatatttggc gataaagggc
     5221 tcgcaagttt cgtcgatgca actctactgc taatccgccg atcagcaccc tgctttttcc
     5281 gcaatcctca atgctgacct catctaacat gtccataggg tactttcgcc gagaggcttt
     5341 gcgcacggcc ttgcggatgg ctgaaagata ggagagattg tctttgatat agctgtcgac
     5401 ctcaccacgc aggatgatgt ctccatgacc ttgaacaata ttttctagcc ccattcgcgc
     5461 cacttttttc aatgaggcgg tcaaatcgtc gatatcacca tctacaatat atggtaaagg
     5521 catgcaggca tcgcctgcaa agagtacccg atcttcttcc accaaaatgg cgatgttatc
     5581 agcgcagtgg cctggcatcg gcattaagct cagcgttttc ttacctactc gtaagcctag
     5641 ttgaccttcg tcaaagatga tatgaggcaa gatgatttta atattgcgaa aggctagatt
     5701 ttgcttgtgc atttcgttaa tggagggagc accgcgctcc tctagccact tacggcacag
     5761 cgcatgtgca atcacggtgg cacccggaaa gaaataattt ccccaggcat ggtcggcgtg
     5821 atagtgagta tttatcacat agcgaatagg aactccaagt tcctgctcga taaattcgcg
     5881 gattgctagc gtctcctcgg ggagagccag cgtatcgatc actatcgccc agctaggacc
     5941 aatcactgca cctgcagtta cctgggcgta aaggtcactt tgaaaggaat aaacgttgtc
     6001 agctacgcgt tctctttgta tcatgatcag tcaccattta taagtgtgtt ggtgtagaat
     6061 agcataatcc tggggtgtaa gtcaagaaaa attaatcttt ttccaatgtt gaccacatat
     6121 accaagccgg ttataatttt gccaacatct gaatagccat atccaggagg agtaagcagg
     6181 caagacctta caccagagaa gggaaggcca ctggctggaa gcccccgtga gtgtctcctg
     6241 ccgaaggtcg cctggattaa ttgctgaaga aatcgagcgt aatgcttcga gagtagaacg
     6301 gcacgggaag cccgttacag cgaagccctg atcggtatca aattactcca gtaaggcggt
     6361 tgagattttg ggagatctgt cagggtgata agagcgccag ttaatgcctg gcgaatagag
     6421 gtggtaccgc ggaaggcgcg ccctttcgtc ctcgtggacg taagggtgtt ttaatttgtg
     6481 ggctacaata tgtgtctagt aacatatttt ctatcacaag tcttcaccca ctcttcagat
     6541 agcacaaggt tacaaatgat tttttagcac tttagaggaa ctaagatgcc cgaattctct
     6601 cttccaaaga cttacgattt taaggccgtc gaacagcgta tctacgagtg gtgggaccgg
     6661 atggggtatt tcaaaccctc aaacgacccc aaaaagccgg gttttgactc gtccaaaaaa
     6721 ccctatgtca tttccatccc ccctccgaat gtgaccggtg agcttcattt gggtcattcc
     6781 atgtttgtct cgatggaaga cctgatgata cggtaccacc gtatgaaagg tatttccact
     6841 ctgtgggttc ccggtagcga ccatgcgggt atagctaccc agttgcaggt ggagaaatcc
     6901 ctagccaaag aaggtttgac acgagagcag attggccgcg aagagttttt gcgccgcacg
     6961 tggttgtgga aggataaatt tggcggaata atcaccggcc agattcgccg gctaggtgca
     7021 tcttgcgatt gggagcgaga gcgttttacc ctcgacgagg gcctgtcccg cgcagttcgc
     7081 gaagcctttg tgcgattata tgagaaaggt ttgatttatc gcggtccgcg acttatcaat
     7141 tggtctcctg gattaaagac cgcagtatca gacctggaag tagagtattc ggaggaacct
     7201 ggtacgttgt atttctttaa atacatactg gaggataatg agaaagagta tctaccagta
     7261 gccaccaccc gtccagagac catccttggc gatacggctg tagccgtcca tccgcaggat
     7321 tcccgttacc agcggtttat tggacggaat gtaatcgtcc cgattctggg tcgagctatt
     7381 ccggttattg cagatgaata tgtggaccgc gaattcggtg gaggtgcgct aaaagtcaca
     7441 ccagggcacg atcccaatga ctatgccatc gccgaacgac atggtttacc aataatctcc
     7501 gtcctggacg aagcaggtcg tattaacgag aacggtgggc cctaccgggg gcaggaccgg
     7561 tttgaaggcc gtaagaattt atgggatgat atgcgctcgg ctggattggt aattaaggag
     7621 caaccataca ctttaaatgt gccccggacg caacgtggcg gcgaaatcgt tgagccaatg
     7681 atatcgaccc aatggtttgt gcatatccag cctttggcag atgccgccct ccaagcggtg
     7741 cgcgatgggc gcatccggat tgtgccagaa cgattcacga aagtatatta caactggcta
     7801 gagaatatcc aggattggtg cattagccgc cagctatggt ggggtcaccg tatcccggta
     7861 tggtattgcg cagattgcgg ggagatgaca gtaactcgca ttgacccgga aagttgtgct
     7921 cactgtctaa gtccgcgaat tgagcaggat ccagacgtgc tcgatacctg gttttcatcc
     7981 ggtttgtggc cattttctac gcttggatgg ccggatgaga caccggatta caaatatttc
     8041 tatcctacaa cgatgatgga gaccggatac gatattttgt tcttctgggt ggctcgcatg
     8101 atcatgatgg ggttggaatt taccggcgag atcccctttt ccactgtgta tttgcatggc
     8161 cttatccgag atgaggttgg tcgcaagatg agcaagactt acgggaatgt gattgatccg
     8221 ttaactgtca tggatgagct ggggacggat gctctgcgtt ttactttatt agtgggatca
     8281 acaccgggca acgatatgaa ccttagtatc aagcgcgtgg aggccaatcg caattttgct
     8341 aataaaatct ggaatgtagg caggtttgtg atctcctcaa ttcaagagtt cgaatcagat
     8401 aataggtgga atgcagagcg agagaaatct tcgtctccaa ttccatatcc ttcgcatact
     8461 tggaccgtag cagatcgctg gatttatgct cgattggcag ctttgataag cgatgtggag
     8521 cgcctttttc agaattacca atatggcgag gctggcaggc agatctacga attcttctgg
     8581 aatgagtttg ctgattggta catcgagatt gccaagctgc agttagctag taaggccgga
     8641 gatggctcgg gtgcggtaga tcgggaaggt gcgcatgttt cacctgccgc caattataca
     8701 aaatccatta ctgctcatat gctcgtgtat gttttggata cctgcttgcg gcttctccat
     8761 ccttttacac catttgtaac agaggagcta tggggacacc tgaagcgcgc tcttggccaa
     8821 acttcattga gttaccaaat gtcttctgaa gctctaattg tggctaggtg gcctgaacct
     8881 cccacgcgag aacccggcga aaaagaagcg gtcgagcaat tctccctgat acaagatgtg
     8941 gtgcgcgcta ttcgcaattt gcgggctgaa aagagtgtga agccaggaaa gcgtatccct
     9001 gcactgatca acgccggtga cagagcacag gtattgcgcg ctcaggcgga ggcaatcgcc
     9061 tccctagccc agcttgatga acaggcattg aatatccaag agtcaattgc ctcgcgtgaa
     9121 gaagggacca tcgcactggt agtgagtggt atcgaagtat acctgcctct agcggggctg
     9181 gtgaacctgg atgaagaacg atctcgcctg gagaaagaac tagcagatat ggaacgccaa
     9241 cgaaaaagag tgcaagaatt gctgtcgagc ccctttgcgc aaaaagcacc gccggatatc
     9301 gtccaaaaag aacgagataa actggcggtt ttagatgaaa ctgtgcagaa actcagggag
     9361 cagttaaaat caatctcgtg aagatcgccc attatcgggt cggcataggt gatagaaaaa
     9421 ttcattgtcc ttgaagtatt tattgaggtg agctatgcga gttcgtctta aggatgttta
     9481 tcttgctgtt gaggagaggg gtgatggtcg cccgttgctg tttatccacg gttatccctt
     9541 gaggggtgcc atgtgggcgc cacaaatcga aggcttggca gatgtggcgc gggtaattgt
     9601 tccagacctg cgcggtcatg gcgaatctga tgccacaaca ggtgcgtact caatggattt
     9661 gttggcagaa gactgtcgag aaatgttaga tgcaatgggc attattgagc ctatcgtgat
     9721 ctgtgggtta tcgatgggag gctatgtgac ctttgccttc tatcgcaaat atccggagcg
     9781 tgtggcgggc ttgatcctgg cagctactcg ggctgtagat gactcaccag aaggcaaagc
     9841 caatcgcgat aaagccgctg atctcgctcg ccagggtggt acgggcgcaa tcgtggaaag
     9901 catgttaccc aaaatgtttg caccgaaaac atacctcgag cgtgcggatt tagtacaaaa
     9961 tttgcgggag atcatgcaag gtgtgtctct cgatggaata ctaggcgatc ttatggggat
    10021 gaagtctcga cctgattcga cccccacctt gggtcaaatt gataaaccaa ccctgatctt
    10081 gcacggcagt gacgatcaac ttattccagt gtctgatgca agttcgatgc aatcagttgt
    10141 cgcaggatca gttctgcgcg tgttgcagga ctctggtcac ttgttaaatc tcgaacaacc
    10201 cgatttgttc aaccaagcca tacgcgaata tcttaaggag ctccatagat gaaatttggt
    10261 gtgatttatc cgcagacgga atatggttca gatcctagcg caatcaaaga ttttgctcag
    10321 accgttgaag atttgggtta tgctcatatc ggttgttttg atcatgtgtt aggtgtcaat
    10381 ccagaccgcc ctggtggatg gcagggacaa tacacatacc aatcagcttt tcaagaacca
    10441 ttcacactgt tcagtttttt tgctgcagtg acacaacgta ccctctttac aacgggagtt
    10501 ttgatactgc cacagcgaca gacagcactg gtggccaagc aggctgccac tctggatgtg
    10561 ttgagcgaag ggcgcctgcg actgggtgtg ggtctgggtt ggaacgcagt tgagtataca
    10621 tgcttaaatg agaattttca taaccgaggg cgccgcttgg aagaacaaat agaggtcctg
    10681 cggttgttat ggactgagcc gttagctagc ttctctgggc gatggcacac gatccctgat
    10741 gcaggtatca acccgctacc ggttcagcga ccaatcccga tatggtttgg gggacgcgcc
    10801 gaaccagcct tgaaacgtgc tgcccgacta ggcgatggct gggtagcccc ataccgaaca
    10861 cctaccgaag cgatgcctaa ttttgaaaaa tttgcccaat acctcgatga ggtggatcga
    10921 agctgggaaa aatatagtgt cgaggtaagg ctgtattatg gaccgggaga tccggatatc
    10981 tggaaaaaac ttgtccgtga atggcaagtg gttggggttg acaacatttc attcaacaca
    11041 atggggtctg tatttaactc acctgaagag catttggcag caatccgcca gtttgctgag
    11101 gagatgggct tgcatgaata agaaagaaat aatcgctgct atgcaaaata gccacgagct
    11161 tttggtagaa atgcttaatg agctgaacac agaagagatg gacgtgccag gtgtagttgg
    11221 caactggacg atcaaagata ttttggcgca tcttacccgt tgggaagcag aactagtaaa
    11281 gctgttatgg caattacgtc aaggtaccca gcccacaacc atcctcaacc aagacatttc
    11341 ccttgacgaa gttaatgctc gctggcatga agaggataaa gatcggccat tggatcgcat
    11401 actgaaagac tttcatggtg ttcgcgagca gactatccgc cgcttggatc atttcaagga
    11461 tgatgatctg actaatccgc agcggtacac ctggcttgga ggtcggcctt tgtggatgtg
    11521 ggtggcggaa gatagttttg aacacgatct tgagcacatg gagcagatcc aggcttggcg
    11581 agaaaatagg ggttagtaga ggcttcctgg ttggagagat atattgactt tggtcaggct
    11641 tgatcctccc aaagtctcta tttttataat gatatgtagc tctcagggca attgcatatg
    11701 ccctgagggg catgcgtaat cccgcaaaaa gagtgtttgg ggtgttttgg tgggggtatg
    11761 cccccgccaa aacaccccat tttctctcct caatggtggg aaatcgccca aatgtgattg
    11821 ccctaatgta gctcatgtgt tctgtttcgg tcgtggtatt tcatgttaaa atggatcaac
    11881 ttaagtatct gaggtggatt tatgtcccaa aaagaagtta ttcgcacaaa caatgcgccg
    11941 aaagccattg gtccctacag tgtggcaaac cgcatcggga acctggtatt tacggctgga
    12001 caaatcggat tagatccgac taccaatgaa ttggtttctg ggggcattga agctgaaaca
    12061 cgccaggcat tgactaatct caagaatgta ctggatgcag caggctcttc gctcgagcaa
    12121 gtggttaaaa ccacagtttt cttacgcgat atgggcgaat ttgcccagat gaatgcaatc
    12181 tacgctgaat tcttccctca aaaccatccc gcacgatcga cagtgcaggt cgctgcttta
    12241 cccaaatatg ccgcggtaga gatcgaggtt gtagcgacaa tttgatcgat agcctaaaca
    12301 gctgatccca taatatatcg tgctgcaaag ttgttatcgc tattttttac aaacctgcat
    12361 ggtgatgccg tgaagtacta atatagatgg ctagtattct tgtggttaag taccccttgg
    12421 tatacccaat cgagtctttc atgagcaaca aggcatagag acatgcccac tgtaacgatt
    12481 attgtgccat gttacaatga gcaagctacg attggacttc tcttgcaggc gatttatgat
    12541 cagtcgtacc cacgagtcca aatggaagtg gtgatagctg acggcctttc aaccgaccaa
    12601 acgctctcac aaatccaagc atttacacaa acccacccgg atttaaaggt tcgagtggtc
    12661 gaaaacccgc agcgaaccat tccctctggg cttaatcagg ccttgagatc tgcactgggt
    12721 gatataatca tccgcctaga cgcgcattca attccgtatt caaattacgt cgctggttgt
    12781 gtagcagctc tccaaaacca gtgcggcgaa aatgtgggtg gggtgtggga gattctacct
    12841 ggtggaagtg gatggctagc gaaatcaatt gccgccgctg cggcgcatcc atttggggct
    12901 ggggatgcgc gttatcgtat cggtggctta gcacaagagg tggatacagt gccctttggc
    12961 gcctttcgcc gtactttgat cgagaagatc ggttattttg acgaaagcct cctgaccaat
    13021 gaggattacg agttcaatgt ccgtatccag cagtcagggg gcaaaatatg gatggatccg
    13081 cagatccgca cccgctattt tgcccgcgca aatttgagtg cgttatggaa gcaatattgg
    13141 cggtatggtt tctggaaagt tcgcatgttg aggcgttatc cgcagtcctt acggtggagg
    13201 caagcactcc ccccattatt tgtatttagt ttgatcctat ttgggatctt ctcattttgg
    13261 atgcctttct ttcgctggct tttgattttt caactgatca tctatagctt ggtaattatt
    13321 ttggcaggtg tacggaaagc acttagcaag cgttatatgc cttatgtctt gggattacct
    13381 ttagccttgt cggtgatgca cctggcttgg ggtagcggtg cgcttttgag catgcttcga
    13441 tatgaaaagc cctaattatc tgcagaaatc tcatccgtgg cgcttacgac ttcaggagcg
    13501 ccgtacgttg ctgctgatgg gtgacctaat tattgccata attagcctgg tgctggcttt
    13561 gtatttctgg ggcatcgctg accgcttccc aggttggtcg cttgaattca tacagcgcag
    13621 agttccaggt tggttttttt tgttcccaat cttttggtta atcttactgg tcgaattgta
    13681 cgatgtgcat cgtgctggta attggcgagc aacgatcagc ggagttgcga ctgcaacctt
    13741 aattggtttt gggctatatc tcttgcttta tttttcctac ctagacccac caaaatcact
    13801 gctcccgagg cgcggtgtgg ctggattctt catcgctgcg ccactcttga ccctgatctg
    13861 gcgtttattt tatatccgta tattcaccga tccgcagttc atgcgacggg tgcttctaat
    13921 tggaggtgga aaagctggtg aaaccatgct gaagatcatc aatgatcttt ggcctcctcc
    13981 tttctatgtt gcgggaataa tcgatgataa tgtcgccaaa gtgggtacga ccatcgagaa
    14041 tcagcaggtt cttgggacca gcgaatcttt attagaattg attgaacatc ataatgtctc
    14101 tgatttaatt gtggcaatat caggcgaaat gaaatcaggg atgttccaag ccatgctgga
    14161 ggcgcaagag cgtggtgtcg agataatccg tatgcccaaa gcttacgaag atatgcttgg
    14221 acgggtgcca atccgattac tggaagccga ttggatcctc cgctcgttcg tggatgaaca
    14281 tcatgtaagc ggattctacg aaatggtaaa gcgcatcata gacatcatcg gaggccttgt
    14341 gggagttgtg atgctgattt tactcatgcc ttttgcggca gttgcaatct ggcttgatga
    14401 tggtctgcca attttctaca ctcagtttcg tgcaggcaag ggttcccagc cttataaaat
    14461 tcttaaatta cgaacaatgc gtcgggacgc agaggctgca ggtgtgccgc aatgggctag
    14521 agaagacgat gctcgagcca cacgagtagg gcgttttttg cgtaaatccc atctggacga
    14581 attacctcaa tttattaatg tggtacgagg tgagatgagc ctggtcggtc cccgctcgga
    14641 gcgacccgag ctgatgggtg tttttcagca atcggtgccc ttctaccgag cccgactgtt
    14701 ggtgaaacct ggcatcacag gatgggcgca agtcaatttt ggttatgcct ctgatatcga
    14761 ggagaccaca atcaagttag aatacgattt gtattacatt aaacatcgcg ggctgctgct
    14821 ggatttgatt atcatgttgc ggacgccagc aacggtattt ggatttagag ggcgataaga
    14881 tggcagatca gacacaatct cctaacggct cgcgtcgttg gtttgctaaa atctggtcac
    14941 catgggttcg ggcgaggatt ggatcaaggt ggctgctatt aattggcgac ctgttgctaa
    15001 ttggtgtggc agtcatgggc agctttggac ttcgtctcga gtttggctct cgctttatct
    15061 tcttttaccc ccaagccctg cggatgatgc tgctggcgat gctcattaag ccactggtgt
    15121 attacatttt cggcatgtac cgcatgatgt ggatgtacgc cagtgtaaag gaaattcgcc
    15181 tcgccatact ctctgtatca actggctcgg ttttactttc gattgcagtc attgtcatga
    15241 cctcaatcca gagccgtctg ccgggatatc tcggattccc gcgcgcggta ttggtaattg
    15301 attggctcct ctctctcata ttagtcggtg gtttgcgttt ttctttacga atactggcag
    15361 aattgggtag cggtcccatc gaggtaaaag ggcacgttcg tcatgtatta atcgttggtg
    15421 ctggcgatgc tggtgcgctg gttgtgcgcg agatgcaaaa aaatccgcaa ttgaatttca
    15481 agccagtgtg ttttctggac gatgaccctg gaaaacaaaa gcaacaaatc catggtgttt
    15541 cggtggtggg ccgcctggat gaactggcgc gggtaattag cgtccgccat gtggacgaag
    15601 tggtgatcgc aattcccagt gcgcctggta gtgtgatccg gcaagttgca gatgtatgcc
    15661 gtttgaagag tttgcctttc cgtacaatgc ctggaatatt tgaattaatt ggtggtaccg
    15721 tcagcgttag ccgcttgaga gaagtggaaa tcaccgattt gttgcgcaga gaacccacaa
    15781 agatagatga gaaaatcatt ggatcgagtc tgggagggcg ccgtgtaatg gtcaccggtg
    15841 cgggaggatc cattggaagg gaattatgta ttcaaacggc tcgctgggga ccctctgaac
    15901 ttatcttatt gggtcatggc gagaacagta tttttgagac actgatcgag atcgaggaaa
    15961 atttcccatc catccctatt catccagtaa ttgcagatat tcgcgacctg gatcgattgt
    16021 cggttgtttt tgacgatctg cggcctcagg ttatatttca tgctgcggcg cataaacatg
    16081 tccctctcat ggaagcaaac gttgaggaag ctgtatcgaa taacgtgctc ggcacacgta
    16141 atatcgttga ggtgagtcaa aattttggta ttgagcgttt ggtgatgatc tcaactgata
    16201 aggccattcg accaaccagt gtcatgggag ccaccaaacg attagcagag ctgattgtac
    16261 tagatgccgc tattcgcact catcaggctt tttctgtggt gcgctttgga aatgtgttgg
    16321 gaagccgagg gagcgtcata ccgaaattca aacgccagat tgccaagggt gggccggtga
    16381 ccattaccca cccagatatg gaacgctact ttatgacaat ccctgaagcc gtgcatcttg
    16441 tattacaagc ttcatctatg ggcgaggggg gagaggtgtt tgtgctaaat atgggtcagc
    16501 aggtgcgcat cctcgacttg gctgaggatc tggtgcgtct ttctggcctc gagccttaca
    16561 aggatattga tatcatattt acaggaatcc gtcctggaga aaagcttagt gaagatctat
    16621 gggatcaatg ggttcaattt gagccaactt cacaccctga tatcctccta ttaaatgatg
    16681 aagacatcat tagtgggcaa gaattgcgtt tcaaggttaa cgatttgctc catcttgccc
    16741 gtgaagggga ttctactgct atcataagca ttttagacga atttatacct ggggctgccg
    16801 ttcgcagcac acctccacca gacatgacgt ccgttgtata gttgactaaa aagtgtctca
    16861 atagcatgga cagaggtgat ttgcagtgat aaagtcacta aaaattatcc ccaagaccat
    16921 tattgagatg agtatcagtt tatgcccgaa gtttcagcaa gtgaatggga taaatttcta
    16981 tcctcttata aggatgtcca catcctgcaa acctccaatt ggggcgagct taaaaaagcc
    17041 ttcggctggc aggtagtacg ctttgtgtgt acagctccat cccaatgtgg ggcacagatt
    17101 ttgtttcgcc gattgccttt tggcatttcg cttgcgtata tcccaaaagg tcctgtgggg
    17161 aaagagtgga gcgcgttatg gtctgagatt gattccgttt gcctccagag aaaggctata
    17221 ttcttgaagg ttgagcctga tctttgggat ggtcaagcgc tggataatgt gcaaggagat
    17281 gctcctacgg gatttcaggt aggtgtgcaa actatacaac caccacgcac cctggtagta
    17341 aatcttgatg gcgatgaagc gcaagttatg gcacgcatga agcagaagac acgctacaat
    17401 atccgccttt cccataagaa gggcgtggta gtacgtcctt ctgcggattt acagcttttt
    17461 agcacgctga tgcgtataac tgcggaacgc gatcgctttg gcgcacatag ccaggcatat
    17521 tatgagaaag tctacgatct gtttcatcct cgcggagagt gtgaactgct gctggcagaa
    17581 tatgagggcg aacccttagg tgcattgatg gtactagcca gtggcaagcg agcctggtac
    17641 ttttacggcg cttctgcaaa tagccatcga gagcgaatgc catcttactt attacaatgg
    17701 gaagcgattc gatgggcgcg tgcccgcggt tgccttgagt atgatctttg gggtgtgccg
    17761 gatgcttccg aagaggtgct ggaggcgaat tttatggccc gttcggaagg tttatggggc
    17821 gtttatcgct tcaagcgtgg ttttggaggt caattgcgcc gatccatagg tccctgggat
    17881 cgggtatatt catccgtgat gtacaatctt tattctcggg tagctggggc tagagtgggt
    17941 tttggctaga gaagagagca acagatcttg gaccattcta tcgtcaatgg agctggctgg
    18001 aatacactaa tcgcatcttt ccccgatcct catttgttgc agacctggga atgggcgcaa
    18061 gttaaatccc gatttggttg gaatccgcta tatatggtgt ggaaacatcc aggagaagcg
    18121 ggagcggtca tcagggatct gactgcatct acccttggaa ggcagacacc gtttgcagca
    18181 gcactggttc tgcaacgcaa tatcccaatc cgcatattgt cagccaatct gcgggtgctc
    18241 tatgtcccca aagggccttt gatgcaagac tggggaaatt cttcccatcg ggaccgcgta
    18301 ttccacgatt tgaaagagtt aggtcgcaag caaggggcta ttttcctcaa aatcgatcct
    18361 gacatacgtc tcggcaaagg gatcccaggg gaagagggag cacaagaatt tgaattagga
    18421 aaccagattt ctaactccct caggcatcaa gggtgggttt tttctagtga gcaagtgcaa
    18481 tttcgcaata cacttttggt agatctcaaa ccgtctgagg agttgttatt ggcggggatg
    18541 aaacagaaga cacgctacaa tgtccgcctt gcgatgcgaa aaggtgtgtc tgtgcgcata
    18601 ggcacccagg atgatttggg acttttatac cgcatgtatg cagagacatc ggtgcgggat
    18661 ggctttgtga ttcgagatga gagttattac cgattcctct ggcaaacatt tatgtctggt
    18721 gaccggtccc cagatattag cgatggacct cgcgcgttgc cactcatcgc ggaagtgtct
    18781 ggcaatccag tagcagcatt agtattgttt tatttcgcca aaagggcatg gtatatgttc
    18841 ggtatgtcgc gtgaagcgca tcgcgaaaaa atgcccaacc acttgctgca atgggaagcc
    18901 atgcgccgtg caaaacttgc aggctgcact acgtatgatt tatggggtgc gcctgatgag
    18961 tttgttcaaa gcgatccttt gtggggtgtg tatcgcttta aagagggatt agggggagta
    19021 gtagttcgtc atcttggcgc ttgggatttg cccattaggc cagttatcta caagctatac
    19081 accgaattac tgccccgtat attggcaatc atgaggcgta gaggtagatc ccggactcaa
    19141 aggatggttt caatctagaa atcaaaggtg gagagaatga ttaatttgcc cagagttaag
    19201 atagcctatc tgcccactcc tgctcacgcc atgccgcgtt tatcagctgc gttgggtggg
    19261 ccgagattgc tcatcaagcg cgatgatcaa acgggtctgg ctttcggagg taataaaacc
    19321 cgaaagctcg agttactgat ggcagaggca caggccaatg gtgccaaaac gctggttact
    19381 gctggcgccg tgcagtcaaa tcactgtcgc cagacggcag cggctgcagc tcgcttcgga
    19441 atggattgta ttcttgtttt ggcgggtgaa aagccgattg aaccttctgg caaccttttg
    19501 ctcgatcatc tgtttggggc tgaattaata tggactcagc gcgatcaacg tgatcggcgt
    19561 ttgcaggaag tgttccaaaa tgcctgggaa gaggggcgca gaccttacct ggttccatat
    19621 ggtggatcca gcccaacggg tgcagccgct tatgcgcttg caattgagga gttgatcgaa
    19681 caatgccaga ccattgagct tgaaggtcat tctccggact ggattgtttt tccctcgtct
    19741 tccggaggga cacaggctgg attagtagct ggagcacgtc tgtttggata cggggggaaa
    19801 atcctgggaa tcagcgtgga tgagccagcc gataggctga gacaaaatgt ggctgagtta
    19861 gctggtcgaa cagctgagca tttaggagag cagatatcct tcgtgcctga cgagattctt
    19921 gtagatgata gcttcatagg cgatggttac ggcatcatga gcgagaaaga gcgggaggct
    19981 atccatctat ttgctcgatc agagggcttg cttctcgacc cggtttacac aggcagagct
    20041 gccgcaggtc tcatcgacct tatccggcgt ggattttttc aatctgacga gacggttttg
    20101 ttttggcaca caggtggaac accggcattg tttgcggatc ggtatcgcaa tctggtcgaa
    20161 aatctctaag gctttctctt gatacccaaa acttcataaa cggcaatcgg tttacgcttg
    20221 cctttaacag ctagcgaccg cgctggcatg atattgaatt ggtcggcaac tagatcataa
    20281 acctgcgcgc tgatcaggat ctgcccggcg tctgcattct cttgaatgcg tttagcggtg
    20341 ttgacgctat caccaatggc tgtatattcg attcgcttct cggtgccgat cagacccaga
    20401 atggcctcgc cgtaatgtat acctgctcca aacgacaggt ggagttcgcg tggtagctcc
    20461 cattgtagga gctggacggc ttcgcgaata tctagggcag ttaataccgc ttgtattgca
    20521 tggtcttgct gtggtattgg ggcgttgaac caggccatga cggcatcacc cataaacttg
    20581 tcaatcgtac cctggtgcgc cagcaccgca tcagcggctg cagccaggta gcggttgagc
    20641 actcccacta attgttcagg agattgtctc tcgctaaagc ctgtaaaatc gcgtatatct
    20701 acgaacaagg tggtgatatc ggtacgcttt cccccaagct gaagcttatc tggatcaagc
    20761 tggtcaatga cggctggcga caccatgcgt tcaaagagtc gtctttgtgc ttgcaagcgc
    20821 tttttttcgg tcaggtcgtc caggacgatg gctactcctt gcgtggtttt ggatgcgtct
    20881 ttcagcgggg ataaattgaa tgaaagtgaa atctccttgc cttccgagag ctcggaatca
    20941 atctcaatgc cgacgatatt ctcgtcagct tggcgaaccc gctcgatgta catctccaat
    21001 ccttggattt gatcaggaag aatatctttc aggtttcgtc caatcatcga atcttgggtc
    21061 tgacccaata tctgctcagc cgcgtgattg cacagggtaa tctgctcctg gatatcggtt
    21121 gtaattacgc cactggcgat ggaggcgaag acattgtcca tcaggttctt caactctgta
    21181 acctcagaca aagtctgccg cagcgattcg aataagcgag cattttcgat ggcgacagcg
    21241 gcttggttgg cgaaagcgga tagaagatcc agctcatatt cggtgaagac gccgctgtgg
    21301 atgcggttgt cggcatagat taccccggtt agttcatcct ttaccttgag cggtatgcac
    21361 agaatagatc gtagatggta agctattaca ctttcttgac cgccaaagcg ggggtcttct
    21421 tgagcgtttg tagttaacac cggcttacga tcggaaataa cgcgcttgat cacagtgcga
    21481 ctgattgcag tttctgagag ttgtactgtt tcttgtgtcc agttgcgagc gacgcggata
    21541 gataactttc cttgagtatc cttcagcatg agaaagccac gctctgcctt cagtaagcgt
    21601 atgatggtgt ccatgacggt catcaatact tcgtcaggat cgagggagga attaatcact
    21661 tgaccgatat tgatcagtga caggaggtct ttatgatgct tttctaggag ggctacatgc
    21721 tggctaactt ttgagaggtt attttttagc tgaccgaatt cgtcaaagat ttgggtgggt
    21781 aaagaggtgg gatcttggtt tatagattgg cgagaagcct cggttaggtg actgagtcgc
    21841 gtgatgagtt ggttgagtat atgcgctaaa gatgtttcgc tatccatgtg ccatctcgac
    21901 atactgaagt gggaaattcc gtaacaaata ggatgaggta ttatacttcg ctgggactat
    21961 taccagaaag ctcaacagac gagtgcaaac ataagtgcca ggctatccgg tacattaatt
    22021 acccagcgca accgagctgg gtatttctac caccttgtta ccaaagagcc tttttatact
    22081 ccaaaccggc gtcgtatgta cgctcggagg gaaatcctgc ggatttcttg cccagcgcgc
    22141 tgagcgatga tttggtttgt taaaccatgt tggctgtaga tggtgttttg atattcggtt
    22201 agcactaaat gggcaggttc tcttgtggac ggcaatctat ttaccagcaa agtcgtgcgt
    22261 tcggaagggg tatgggttgg ctgggggcgc ttacccagcc ggacaagtgc caagttaatt
    22321 tgctggtaag ctcgttctaa tggagaaagt gaagccataa gggcgatctg ggagagtata
    22381 gccggcggct tgagtcctgc gcgcagcaag gctttctcta acgcgattga aaggaaaggt
    22441 atcctcttgt gcaattgctt tctgcggatc aaggggatca acagggcaat catgatagca
    22501 aataaacctg cgataataat gatatacagc ctttcctgcc agatatttgt gcgctcaaaa
    22561 gggttgtcca aggtattgcg cggagtgggg ggctctggct caggatcctc gcggtcgaga
    22621 ttcgagctgg ataaattatt tagattatct tgtccaagcg ccgttaatgg acgagataat
    22681 tctggttggg aaactgttgg ctcgaattca acccaaccga tttccggaaa gtaaacttcc
    22741 ggcca
//
LOCUS       DBNGSX010000326         3189 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_512122, whole genome shotgun sequence.
ACCESSION   DBNGSX010000326 DBNGSX010000000
VERSION     DBNGSX010000326.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_512122"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="AC16CB_09885"
     CDS             <1..216
                     /locus_tag="AC16CB_09885"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR3342819.1"
                     /translation="GTNYGINRFSHNSLLDEKIGGSFHMALGAGYPESGSQNKSSIHW
                     DMICDLRQDSEIRVDGELMYRNGQFVI"
     gene            complement(298..1092)
                     /locus_tag="AC16CB_09890"
     CDS             complement(298..1092)
                     /locus_tag="AC16CB_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342820.1"
                     /translation="MRIQLPLLVLIVILSLNACTLPISLPGDIQTAIIKTESSNPTST
                     TNPEHGNPPTEELTRTLEPTLATSLPPTVQPSPTPTPDLRVIIGKPEDYILQKEDLPD
                     KYILKPGWSTPHNNSEILSARGVVEGKAYLEATGRIGGWIVYYDLVESTTIAPEVIES
                     YLVMYDNVYGSKIAKSPEWDTLEENQEIIDIDLVLGEWNRVTIMRERQSNGKYYVWYA
                     IEFIYRNIWTEVMAGGMESDVQHDYVENAARVVLAKLETAPLGEPK"
     gene            complement(1336..1863)
                     /locus_tag="AC16CB_09895"
     CDS             complement(1336..1863)
                     /locus_tag="AC16CB_09895"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HIR3342821.1"
                     /translation="MEILLSSQAWLGAGLIFILRVTDMTLDTLRVLVVMRGRKSYAWI
                     LGFFQAGIFVVAISTVLQNLDNPLNLVGYASGFATGNVVGLLIEQRLAIGHILLNIVS
                     PSRGTAIAEYLRGEGYAVTEIPARGKDGMVSLLYCNVLRRNVDRVRKLVNEVDPEAFI
                     TSEDVRPVMRGFWRA"
     gene            complement(1867..>3189)
                     /gene="glgP"
                     /locus_tag="AC16CB_09900"
     CDS             complement(1867..>3189)
                     /gene="glgP"
                     /locus_tag="AC16CB_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HIR3342822.1"
                     /translation="LERTRELVNSGYDFEKAKEMVRASNVFTTHTPVPAGNDEFPLWL
                     TDKYFAHIWPELGLSRDQFIDLARHSVSWGDSFSMPMLALRFSQGCNAVSELHGRVAR
                     KMWNFVWPQRRADDVPITHITNGIHTGTWLARRMGHLFDRHLGQDWRERVDDPDVWDQ
                     ILAIPDDELWEVRRHLKRKLAYYITDCARNRWVKGGAPPIQILAGGALIDPNVLTIGF
                     ARRFAPYKRANLILRDIDRLLTSLNRPGKPIQIIFAGKSHPDHEGGKMLIQDVYRAVK
                     RPEAGGRLVFLEEYNMNLARYLVQGVDIWLNTPRRPNEASGTSGQKAALNGALNFSVL
                     DGWWREGYNGKNGWAIGEDRDYDNLDQQDEIDAESLYDILEDEIIPLYYDERTADGLP
                     FEWIARVKESVRTLAPLFSMRRMVKEYLEDMYLPGMQSSPSTQKQKGS"
BASE COUNT          791 a          873 c          749 g          776 t
ORIGIN      
        1 ggcaccaatt acggtatcaa ccgcttctcc cataacagcc tgctggacga aaagatcggt
       61 ggctccttcc acatggcgct gggcgccggc tacccggaaa gcggcagcca aaataagagc
      121 tccatccatt gggatatgat ctgcgacctg cggcaggatt cagagatccg cgtggatggc
      181 gagctgatgt atagaaatgg gcagtttgtg atatgaccag tgagacccta ggcccgccat
      241 aatcctcgcc tcactgggtg cacatcctcc gacgtgacga atgcgtctgg gttgacttca
      301 tttgggttca cccaacggtg cagtttcgag ctttgcaaga actaccctgg cagcgttttc
      361 gacataatca tgttggacgt cagattccat cccccccgcc atgacttccg tccatatgtt
      421 gcgatagatg aattcgatcg cataccatac ataatatttg ccgttggatt gtctttcacg
      481 catgattgtc actcgattcc attcccctag aaccaaatca atatcaatga tctcctgatt
      541 ttcttcaagc gtatcccact ccggtgattt agcgattttc gagccataaa cattatcata
      601 cataacaaga tacgactcaa tcacctcagg tgcaatcgtc gtgctttcaa ctaaatcata
      661 gtaaacaatc caacccccaa ttcgtcctgt tgcttccagg taagcctttc cctctacaac
      721 gcccctggcg ctcaatatct ccgaattatt gtggggggtc gaccatccag gctttaggat
      781 atatttatct ggcaaatctt ccttctgcaa aatatagtcc tctggcttac cgataatcac
      841 acgcaagtca ggtgttgggg tcggagatgg ttgtacggtt ggtggcagag aggtagcaag
      901 ggtaggttct aaagtacgag ttagttcttc agtgggcgga ttaccatgtt ctggattagt
      961 ggtagatgtt ggattggagg attcggtttt aattatcgct gtctggatgt ccccgggcaa
     1021 tgatataggc aacgtacacg cgttgagaga caaaattacg atcagcacaa gaagcggtaa
     1081 ctgtattctc attttttcca cctccatctt agatatatgg aatacgccac catacctaac
     1141 gactacccca taaccaattt caacaaatta gcagtcagcg attattaatc actaacccca
     1201 atatcgccct ataggctggc tgtacttatt attgcagatt tctcaaaaat gtctatatgt
     1261 gatcgagaat caaaatgtcc attctgccca cctgtgagat cgcaggccaa agaggagtca
     1321 aagcggaagg gaaaactatg cccgccagaa gcctcgcatc actgggcgca cgtcttccga
     1381 cgtgataaat gcctcggggt cgacttcatt taccagcttc ctcacccgat ccacattgcg
     1441 ccgcagcaca ttgcagtaca gcaggctgac catgccgtcc ttgccgcgcg ccgggatttc
     1501 agtcaccgcg tatccttctc cgcgcagata ctcagcgata gccgtgccac ggctggggct
     1561 gacgatgttc agcaagatgt gcccaatcgc aaggcgctgc tcgatcagca atccaaccac
     1621 atttcccgtg gcgaacccag acgcatatcc gaccagattc aacgggttat ccaagttctg
     1681 caatacagtg cttatcgcca caacgaagat acccgcctga aaaaaaccca atatccacgc
     1741 ataggattta cgaccacgca taaccaccag cacccgcagc gtgtccaggg tcatatcggt
     1801 tacgcgcaaa atgaagatca gacctgcacc taaccaggcc tgagatgaaa gtaaaatttc
     1861 cattaactac gatccttttt gtttctgagt cgagggggag gattgcatgc ccggtaagta
     1921 catgtcttcc agatattcct tcaccatacg gcgcatacta aaaagcggtg ccaatgtgcg
     1981 cacagattcc ttgactcttg ctatccactc gaacggtaag ccatcagccg tgcgttcgtc
     2041 ataatagagc gggataattt catcctccag gatgtcatac aggctctcgg catcgatttc
     2101 atcctgctgg tcaaggttgt catagtctcg gtcttcgcca atggcccaac cgtttttgcc
     2161 gttatagcct tcccgccacc agccatccag aacggaaaag ttcaatgccc cgttcaaggc
     2221 ggctttttgc ccggaggttc cagaagcttc attgggacga cgcggtgtat tcagccatat
     2281 atccactcct tgcaccaggt aacgcgccag attcatgttg tactcttcta agaagaccag
     2341 gcgccctcca gcctctgggc gctttacagc gcggtaaaca tcctggatta acatcttgcc
     2401 gccttcatga tcagggtgtg atttgccagc aaagataatt tgaatcggct tgcccggacg
     2461 gttgagcgat gtgagcaagc ggtcgatatc gcgcaagatc aggttggcgc gtttataggg
     2521 tgcaaaacgt cgtgcaaaac ctatggtgag cacattcggg tcgatcaatg cgccgcctgc
     2581 cagaatctgg ataggaggag cgcctccttt cacccaccgg ttgcgggcgc agtcggtgat
     2641 gtaatacgcc agtttgcgct ttaagtgcct gcgcacctcc caaagttcat catcggggat
     2701 agcgagtatt tgatcccaca cgtctggatc atcaacgcgc tctcgccaat cctgacccag
     2761 gtgacgatcg aacaaatgcc ccatacgcct ggctagccag gtgccggtat ggataccatt
     2821 ggtaatgtgg gtgattggga catcatctgc acgccgctga ggccacacaa agttccacat
     2881 cttgcgcgcc actctaccgt gaagttcgga cacagcgttg caaccctgcg aaaaacgcaa
     2941 cgccagcatc ggcatgctga agctatctcc ccaggaaact gaatggcgag ccaaatcgat
     3001 gaactggtcg cggctgagac cgagttcagg ccaaatatga gcgaaatatt tatccgttaa
     3061 ccacagcggg aactcgtcat ttcctgccgg aaccggtgtg tgcgtagtga acacgttgga
     3121 tgcgcgcacc atctccttgg ctttctcaaa atcgtatccc gagttgacca attctcgcgt
     3181 acgctccag
//
LOCUS       DBNGSX010000327         3153 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_997394, whole genome shotgun sequence.
ACCESSION   DBNGSX010000327 DBNGSX010000000
VERSION     DBNGSX010000327.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_997394"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="AC16CB_09905"
     CDS             <1..459
                     /locus_tag="AC16CB_09905"
                     /inference="COORDINATES: protein motif:HMM:NF051259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dehydroquinate synthase/iron-containing alcohol
                     dehydrogenase family protein"
                     /protein_id="HIR3342823.1"
                     /translation="KDEAAREDMSIAALFSGLALANAKLGAVHGFAGVLGGSLNAPHG
                     AICARLLPVVMSINARALSERAPQSEAFARYDEVAQILTGKANARAADGIAWLYDLVA
                     GLGTAPLSSFGLRRNDFPGIVQKSAQASSMKGNPVQLTQEEMSEILELSL"
     gene            624..1175
                     /locus_tag="AC16CB_09910"
     CDS             624..1175
                     /locus_tag="AC16CB_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342824.1"
                     /translation="MSANELSEDTQHNDSSSDELAAEAINLTDASVESINAEAVRLRM
                     GSVGQLTAQETELRLSAAQQITTLAVNMEESAAAVIVTDQADISDSIIGAIQTNHVSA
                     TNNIFGLVYAQTAEIEEGRAGLIVTRQIQGESVKAAVLLAGRVEGPVEVALDTPRALL
                     AGLAAGVGAGLVLLIGSLSRRRK"
     gene            1310..1951
                     /locus_tag="AC16CB_09915"
     CDS             1310..1951
                     /locus_tag="AC16CB_09915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HIR3342825.1"
                     /translation="MEKIVFKAKHREVIGKQVKALRREGRLPAILYGKSIQPIPVTLD
                     YREISRILPTISSSHLILVDVDGEEYNALIREKQLHPVQGTVVHLDFMVVSLTEKLRA
                     YVSINIEGEAPASRDFNGMVVTGLEEIEVESLPGDLPERIMVDISSLDEIGSAIHVRD
                     LQLPENVKILTDLDEMLVIVTAPEAEEVVEEEEEELEEPEIIEKGKKEEEEEE"
     gene            complement(2011..2631)
                     /locus_tag="AC16CB_09920"
     CDS             complement(2011..2631)
                     /locus_tag="AC16CB_09920"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3342826.1"
                     /translation="MQKSQLNLTEQEISRRLVLTSGDEVENPYPHGFLSHPPRPAAVL
                     IPLLRRYENWHVLYIRRTANSQDPHGGQVAFPGGAADRDDLNAEATALREANEELGLH
                     TSDVRTLGRLIDFITITSYRVTPIVGVIPWPYPLKLETKEVSRAFTIPLTWLADPKNR
                     EERPRTLPGPFEPINVIYYKPYDGETLWGASARFTLELINVLFDPE"
     gene            complement(2603..>3153)
                     /locus_tag="AC16CB_09925"
     CDS             complement(2603..>3153)
                     /locus_tag="AC16CB_09925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016426 - tRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HIR3342827.1"
                     /translation="LISMGIGPGVNVIEAGTGSGALTTALAWAVGDEGRVFSYESRPE
                     MQNLARKNLERLGLHTRVDLKLRDIAEGFDETGVDALFLDLPNPYDYVQQVRSALKPG
                     GFFGCILPTVNQVARLLPDLHQAYFSFVDICEVLLRYYKPVPQRLRPTDRMVAHTGYL
                     VFARPIIPTEPPPDAEIPAKSD"
BASE COUNT          820 a          687 c          869 g          777 t
ORIGIN      
        1 aaggacgagg ctgcacgaga ggatatgtcg attgcggcgt tatttagcgg gcttgcatta
       61 gcaaatgcaa agttgggagc tgttcatggt tttgcaggtg tactgggagg aagcttaaac
      121 gcaccacatg gagcaatttg cgctcgcctg ttgcccgttg tgatgtcgat aaacgcgcgc
      181 gcattgagcg agcgcgctcc gcaaagcgag gcttttgcgc ggtatgacga ggtggcgcag
      241 attttaactg gcaaggcgaa tgcccgagca gctgacggaa ttgcctggtt atatgatttg
      301 gtagcaggtt tgggtacagc tccgttgtca tccttcggac ttcggaggaa tgattttcct
      361 ggaatagtgc aaaaatctgc tcaagctagc agcatgaaag ggaatccggt acaattgaca
      421 caggaggaga tgagtgagat tcttgagctg tcgctataat tactagtgta tgagtcgaat
      481 ccgatctgtg ccgaacaata tttgttcctg cttccggatt ttattctgag ctattgacga
      541 aatcaggcga gaagactaca ataactcgca cgtcggttcc agtgtccgcc tgtactaggc
      601 ggcgtttttt tgccggagta tgtatgagtg cgaacgagct ttctgaggat actcaacaca
      661 atgatagctc atccgatgag ctggctgcag aagcgattaa tcttacggat gcatccgtcg
      721 agtcgatcaa tgccgaagca gtccgcttga ggatgggttc tgtaggccaa ttgaccgcac
      781 aagagaccga acttcgccta tcagctgccc agcaaatcac tacgctggct gtaaatatgg
      841 aggagtccgc agcggcagtg attgtgacag accaagccga tatctcggat agcatcatcg
      901 gtgcaataca gaccaaccat gtctcagcca cgaataacat attcggattg gtttatgcgc
      961 aaactgctga gatcgaggaa ggacgcgcgg gtttgattgt gactcgccag atacaaggcg
     1021 aatctgttaa ggcggcagta ttattagctg gccgggtgga aggtccagta gaagttgcgc
     1081 tggatacgcc cagggcattg cttgcagggc tggctgctgg ggttggtgca ggactagtcc
     1141 ttctgattgg ctctttgagc cggcgtcgta aatgaggttg aacaacagcc agattggcac
     1201 agaatacctg gatggctgtt aatatccacc ggcggaggcc ggatattatc ccaaggagtg
     1261 atggtaacct ggggaatcct gggtgagagg cgctccaagg aaggaagata tggaaaaaat
     1321 cgtttttaaa gccaagcatc gcgaggtaat tggcaagcaa gtaaaagccc tgagacgcga
     1381 agggcgatta ccggcaattc tttatgggaa gagcattcaa ccgatccctg ttacgcttga
     1441 ttatcgtgag attagtcgga tactacctac gatttcgtcc tcacatttga ttctcgtaga
     1501 cgtagacggg gaggaatata acgcgttaat ccgtgagaag cagcttcatc cagttcaggg
     1561 cactgtagtg catttggatt ttatggttgt ttcgttgacc gagaagttgc gtgcctatgt
     1621 ttctatcaat attgaagggg aagcccctgc aagtagagat ttcaatggca tggttgtcac
     1681 tggtctcgaa gagattgaag tcgaatcctt gcctggcgat ttaccagagc gcattatggt
     1741 agacatttct tcgctggacg agattggttc ggcaatccat gtgcgtgatc ttcaactacc
     1801 agagaatgta aagatattaa cagatctcga cgaaatgttg gtgattgtaa ctgctcctga
     1861 ggctgaagaa gttgtcgaag aggaagagga ggaactcgag gagccggaaa ttatagagaa
     1921 aggtaaaaaa gaagaggaag aagaggaata atattgaaga tggggtatat gcggtgtatc
     1981 tacagccaca tataccccat ttttctctgc ttattctgga tcgaataata cattgatcaa
     2041 ttcaagggta aatcttgcgc tggcacccca tagcgtttcc ccatcatacg gcttgtaata
     2101 aatcacgttt atcggctcaa aggggccggg taaagtccgc gggcgctctt cacgattttt
     2161 tgggtctgcc aaccaggtaa gggggatagt aaaagcacga ctaacttcct tggtttctaa
     2221 tttaagtgga tatggccaag ggataacgcc aacgattggt gtaactcgat agctggtaat
     2281 cgtaataaaa tctatcagac gccccaaagt gcgcacatct gaagtgtgaa gaccgagctc
     2341 ttcgtttgct tctcgcagtg ctgttgcttc cgcgttgaga tcatccctgt cagcagcccc
     2401 tccaggaaac gctacttgcc cgccatgggg atcttgtgaa ttggctgtgc ggcggatata
     2461 cagcacatgc cagttttcat atcgcctgag taaggggatc agaacagcag caggacgagg
     2521 cgggtgagac aaaaatccat gcggatatgg attctcaact tcatcgccac tagttaaaac
     2581 cagacgccga gagatctctt gttcagtcag atttagctgg gatttctgca tcgggtggtg
     2641 gttcggtagg aataatcggg cgggcgaaga cgagatagcc agtatgggcg accatacgat
     2701 ccgtaggacg caagcgttga ggcaccggct tgtagtagcg cagtagaacc tcacaaatgt
     2761 caacaaaact gaagtatgct tggtgtagat caggaaggag acgtgcgacc tgatttacag
     2821 taggtaggat acaaccaaag aatccacctg gcttgagcgc cgatcggact tgctgcacgt
     2881 aatcatacgg attgggcaaa tccaggaaaa gtgcatccac tccggtctca tcgaagcctt
     2941 ctgcaatgtc tcgcaatttt aggtcgaccc tggtgtgcaa tcctaggcgt tctaaatttt
     3001 tccgggcaag attttgcatc tctggacggc tctcataaga gaacacccga ccttcgtcgc
     3061 ctaccgccca agccagagcg gtcgtaagcg cccctgatcc agtgcctgct tcgataacat
     3121 tcaccccagg tccaattccc attgagatga gaa
//
LOCUS       DBNGSX010000328         6044 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_442592, whole genome shotgun sequence.
ACCESSION   DBNGSX010000328 DBNGSX010000000
VERSION     DBNGSX010000328.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6044)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_442592"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="AC16CB_09930"
     CDS             <1..273
                     /locus_tag="AC16CB_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HIR3342828.1"
                     /translation="PKGPGAILGFGVRGGLEAGRRFIDRLQLFSHLANVGDAKSLAIH
                     PASTTHSQLTTEQQLSAGVSPDFVRLSVGIEDIDDILFDLDQALSS"
     gene            284..1225
                     /gene="metA"
                     /locus_tag="AC16CB_09935"
     CDS             284..1225
                     /gene="metA"
                     /locus_tag="AC16CB_09935"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015945319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase MetA"
                     /protein_id="HIR3342829.1"
                     /translation="MPVKIPETLPARQILESENVFVMGETRARHQDIRPIKLAILNLM
                     PTKIATETQLLRLLGNTALQIDITLLHMDSHQSKNTPSDHLLAHYRSFRQVQGEKFDG
                     MVITGAPVEHLPFEQVDYWDELKTIMEWTETHVYSTMHICWGAQAGLYSRFGVPKHPL
                     PAKMFGVFPHRVNIRNELLLRGFDDEFYAPHSRHTEIRRNDIQPVGELDILSESDEAG
                     VYIVASRDRRQFYITGHSEYDPLTLKAEYDRDQGNGMPITIPKNYYPHDDPSQLPIVR
                     WRGHANLLFANWLNYYVYQITPYDRKKIPQGGTYATF"
     gene            complement(1256..3022)
                     /locus_tag="AC16CB_09940"
     CDS             complement(1256..3022)
                     /locus_tag="AC16CB_09940"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018743953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR3342830.1"
                     /translation="MSQLFSQTLREAPSEAEIPSHQLLLRAGFIRQLAAGIFSYLPLA
                     KRALTKIEDIMRDEMDAIGGQEVTMPVVHPAEVWQETERWYQIGSEMGRFKDKNNRDM
                     VLAMTHEEVVADLVRKEIQSYRQLPRLIYHIQTKWRDDPRPRAGLIRVREFTMKDSYS
                     LDADWDGLDQQYRAHYQAYFNIFHRCGVSAIAVKSDVGMMGGKLAHEYMYITPIGEDT
                     LLLCNSCGYAANRQIARFKKAPAASEAPQQVEKVATPNTTTIEGLANLLQVTKAKTAK
                     AVFLVASIPEGREVVERFVFAVVRGDMEVNETKLANALGAQSLRPATEDEITAVGAVA
                     GYASPVGLKNALVVVDDAIPSSPNLVAGANEDGYHLLNVNYERDYTANLVTDIAAAQD
                     GDACPNCGGELHTVRGVEVGNIFKLGTRYSDSMGCKYLDRDGVEKPVIMGSYGIGSGR
                     LLACVAEEHHDDFGLIWPITVAPYHVHLVVLGSKEGSDTRLAADQLYGDLLAENIEVL
                     YDDRDESPGVKFNDADLIGLPIRLTISERTLKQGNVEFKRRDSKEKELIPRSDVITHV
                     KNEIQALKAAIQAQVVQVSFDE"
     gene            3352..3942
                     /locus_tag="AC16CB_09945"
     CDS             3352..3942
                     /locus_tag="AC16CB_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HIR3342831.1"
                     /translation="MKKRLFFILILILLFVLAVQPAAAKQLLASFTGTGGNDHYEGTS
                     KDDVIRGLGGNDDLSGEGGNDTINGGNGSDRLDGGSGADSIRGGNGKDIISGGGNNDV
                     INGENGNDIINGDGGNDTLKGGAGNDKIRGGTGNDWIYGDEGKDNLMGNEGNDRIYGG
                     PGTDIVNGGPGYDICIVGTKETKVFECEIVYRWANQ"
     gene            complement(3946..4857)
                     /locus_tag="AC16CB_09950"
     CDS             complement(3946..4857)
                     /locus_tag="AC16CB_09950"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3342832.1"
                     /translation="MSFEKSRPHTARNKRILKLTAFTALSCALLYLIISTSLAGVYAY
                     FLTHPACMTNPPRIPGLPSPEEHWLSTSDGLSLRTWYYPGDNGAAILALGGMGGALGE
                     NLPPVEFLIHAGFGVLQIDSRACARPASVVTLGAKEVLDAEAGLSFLRNRPEVKKIGI
                     FGFSMGAATAVRTAARQVDIAAVVAEGGYFNLGEDFVEPESQINLLYRTFLYTIAGAY
                     QFFSDVNPWEVSPINDLPSISPRPLLLIYGEGEADSGRALAQFDAAREPKRLWIVPGG
                     AHGSNFAASPGEYQSRIVEFFNETLQP"
     gene            complement(4847..5779)
                     /locus_tag="AC16CB_09955"
     CDS             complement(4847..5779)
                     /locus_tag="AC16CB_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3342833.1"
                     /translation="MYEPAYVKLLNNNELAQRVSYAYDMLSKCNVCAWECRIDRLVGK
                     VGICKTAQRAKVSSYGPHLGEEDPLRGWRGSGTIFFTRCNLRCQFCQNHDISQTDKGE
                     EIEPGQLAAIMLELQSLGCHNINLVSPSHVVPQIMAAVLIAAQAGLRIPLVYNTGGYD
                     SVEMLKLLDGVVDIYMPDMKYASAQIARYYSKIPNYPQANQAAVREMHRQVGDLHLDQ
                     NGLATRGLLVRHLVLPHNLAGTENVVQFLAEQISTDTYLNLMEQYRPAYNAQRFPKLK
                     RRTTQQEYQAAVDMAHAAGLHRLDKRKPLNWHVI"
BASE COUNT         1380 a         1533 c         1640 g         1491 t
ORIGIN      
        1 ccgaagggac caggggcgat ccttggtttt ggggtgcgag ggggattaga ggcagggcgc
       61 aggtttatcg accgattgca acttttcagc catttggcca atgtaggcga tgcaaaaagt
      121 ttggcgattc accctgcttc cactacgcac agccaactca ctactgagca gcagctcagc
      181 gcgggcgtca gccccgactt tgtgcgatta agtgtcggca tcgaagatat tgacgatatc
      241 ctgttcgacc tcgatcaagc attatcatcc tgaggaggct cacatgccgg ttaaaatccc
      301 tgaaaccttg cctgccaggc aaatccttga aagcgaaaat gtcttcgtga tgggtgagac
      361 acgcgcccgg catcaagata taagacctat caaactggct atcctgaact tgatgccgac
      421 gaaaatcgct accgagacac agctcttgag attgctgggg aacaccgcgc tgcagattga
      481 tattaccctg ctgcatatgg actctcatca atcgaagaat acaccctcag atcatttgct
      541 ggctcattat cgctcttttc gccaggttca gggcgaaaag tttgatggaa tggtgatcac
      601 cggcgcgccg gttgagcatt tgccttttga acaagtggac tattgggacg agttgaaaac
      661 tattatggaa tggaccgaga cgcacgtcta ttccacaatg catatatgct ggggtgcaca
      721 ggcgggactg tatagccggt ttggcgtccc caaacacccg ctgccagcta aaatgtttgg
      781 tgtatttccc catcgagtga atattcgcaa cgagttactc ctgcgcggct tcgatgacga
      841 gttttatgcc ccccattctc ggcacaccga aattcgcaga aatgatatcc aaccggttgg
      901 cgaactggat atcctatctg aatcggatga agctggggtt tacattgtgg cttctcggga
      961 tcgccgccag ttctatatca ctggtcattc ggagtacgat ccactgacct tgaaggctga
     1021 atatgatcgc gaccagggta acgggatgcc gattaccatc ccgaagaatt actacccgca
     1081 tgacgatcct tctcagctac ccatcgtgcg atggcgcggg catgccaacc tgttgtttgc
     1141 caattggtta aattattatg tgtaccagat cacaccctat gataggaaaa agatccctca
     1201 gggaggtacg tatgcaacct tctaaatgag agatgtgaaa ttagagcagc ccgaattact
     1261 cgtcaaaaga cacttgaact acttgtgcct ggatggctgc tttcaaggct tggatctcat
     1321 tcttgacatg ggtgatgaca tcgctgcgcg ggataagctc tttttctttg ctgtcgcggc
     1381 gcttgaactc cacgttgcct tgcttcaggg tgcgctcgct tattgtaagg cggatgggca
     1441 agcctatcag atcagcgtca ttaaatttca ccccggggct ctcatccctg tcatcataca
     1501 ggacttcaat attttctgct agcaaatcgc cataaagctg gtcagcggca agccgtgtat
     1561 ctgagccttc cttactaccc agaacaacta gatggacatg atagggggcc actgtaatag
     1621 gccagatcag cccaaagtcg tcgtgatgct cctcggcgac gcatgccagc aggcgcccgc
     1681 tgccgatacc gtaggagccc attatgaccg gtttctccac gccgtccctg tctagatatt
     1741 tgcatcccat agaatcactg taacgtgtgc ccagcttgaa gatattgcct acctccacgc
     1801 cgcgcacggt atgcagctca ccgccgcaat tgggacaggc atctccatcc tgggcggcgg
     1861 cgatatcggt gaccagattg gcagtgtagt cgcgctcgta attcacgttg agcaagtggt
     1921 agccgtcttc attggcgccc gcgaccaggt tgggtgagct agggatggca tcatccacaa
     1981 ccaccagggc atttttcaac ccaaccggtg atgcgtagcc agcaaccgcg ccgacggcag
     2041 tgatctcgtc ttcggtggca gggcgcaagg attgggctcc taatgcgttc gccagtttgg
     2101 tctcgttcac ctccatatcc ccgcgcacca cagcaaagac aaagcgctcg accacttctc
     2161 tcccttcagg gatggaggct accaggaata cggctttggc ggttttcgcc ttggtcacct
     2221 gcaggaggtt tgccagcccc tcaatagtgg tcgtgttggg tgttgcgact ttctccacct
     2281 gctggggtgc ttctgatgcg gcaggcgcct tcttaaagcg ggcaatttgc cggttggcag
     2341 cataaccgca agaattgcac agcaggaggg tatcctcacc aattggggtg atgtacatat
     2401 attcatgggc cagcttcccg cccatcatgc ccacgtcgga cttcaccgcg atagccgaca
     2461 cgccgcagcg gtgaaaaata ttgaagtacg cctggtaatg ggcgcggtac tgctggtcta
     2521 agccatccca gtcggcatcc aggctgtagc tatccttcat agtgaattcg cgcacccgga
     2581 tcagcccggc gcgcgggcgg gggtcatcgc gccatttggt ttggatgtgg tagatcaggc
     2641 gcggcaactg gcgataggat tggatttctt tgcgcacaag gtcggcgacc acttcttcgt
     2701 gggtcatggc aagcaccata tcgcggttgt tcttgtcttt gaagcgcccc atctcagagc
     2761 caatttggta ccagcgctca gtttcctgcc aaacctcagc ggggtgtacc acgggcatgg
     2821 tcacctcctg cccgccaatg gcatccatct cgtctcgcat aatgtcctcg atcttggtca
     2881 gggcgcgctt ggccagcggc aggtagctga agatacccgc agcgagctgg cggataaagc
     2941 ctgcccggag caagagctgg tggcttggaa tttcggcttc agatggcgcc tcacgtaaag
     3001 tttggctgaa gagttgggac attctcattg gacgtgctcc tgtgtcaggt ttccagtgtc
     3061 atgtatttag tattctcagt gcataataaa acccccgggc tgtccgtccg agggcgtcta
     3121 tgtaactacc actgaatccc taatggacga aagctcgcat gggcttgcgc ggcgcagggg
     3181 ttggggtagg taaaggatat ttcccttcca tgggtgtcat tagtttatca cgggttgaat
     3241 gaatgtcaag gtcgcctcca atcgggggtt ttgatttgac ggattgggaa ctttttggat
     3301 attattgtcg taatataata agtggtagaa tgaacgtcaa ggagaataaa aatgaaaaag
     3361 cgactattct ttattctaat cctgatcttg ctgttcgtgt tggctgttca gccggcagct
     3421 gcgaaacagc tccttgcaag cttcaccggc acggggggta atgatcatta cgaaggcaca
     3481 tccaaggatg atgttattcg tgggttgggt ggcaacgatg acctgagcgg tgaaggtggc
     3541 aacgatacca tcaatggcgg caatgggagc gacaggttgg acggcggcag cggtgcagat
     3601 tccatccgag gcggcaacgg aaaggacatc atcagtggtg gtggcaacaa tgatgttatc
     3661 aatggcgaga acggcaatga catcatcaat ggcgatggtg gaaacgatac tctcaaaggg
     3721 ggtgcaggta atgataaaat ccgtggcggc accggcaatg actggatcta cggtgatgaa
     3781 ggaaaagata acttgatggg caatgaaggc aacgaccgta tatacggtgg ccctggcacg
     3841 gacattgtga atggcggccc tggctacgat atttgcattg tgggcaccaa ggagactaag
     3901 gtgttcgaat gcgagatcgt ttatcgttgg gctaaccagt aagatctatg gttgtaacgt
     3961 ttcgttgaaa aactcaacga ttcgactttg atactccccc ggcgaggcgg cgaaatttga
     4021 accgtgcgcc ccgccgggga caatccacag ccttttgggc tctctggcag catcgaattg
     4081 ggcaagggcg cgcccgctat ccgcttcccc ctctccataa attaatagca atggtcttgg
     4141 gctgatcgat ggcagatcgt tgattgggct gacttcccag ggatttacat cgctgaaaaa
     4201 ctgataagcc ccggcgatgg tgtaaagaaa tgtccggtat agaaggttta tttggctttc
     4261 aggctcgacg aaatcctctc ccagattaaa gtacccccct tcagcgacca ctgcggcgat
     4321 atctacctgg cgagcagcag tgcgcacggc ggtagcggca cccatcgaaa atccaaaaat
     4381 tccaattttc ttcacctctg ggcggtttcg gaggaatgat agtcctgcct cggcgtctaa
     4441 gacctctttt gcccctaatg tgaccacaga ggcaggacgg gcacaggcac ggctgtcgat
     4501 ctgaagtacg ccgaaacccg catggatcaa gaattccacc ggtggcagat tctcgcccaa
     4561 cgcgccgccc atcccgccta gggcgaggat ggcagcccca ttatcccctg ggtagtacca
     4621 ggtgcgcagg cttaggccat ctgatgtgct caaccagtgt tcttcgggag aaggcaggcc
     4681 tggaatgcgt ggtgggttgg tcatgcaggc agggtgtgtc aggaagtagg cgtacacccc
     4741 ggccagactg gtactgatga tcaaatataa cagggcacag gataacgctg tgaaggctgt
     4801 cagctttagt attcgcttgt ttcgggccgt gtggggtcgg ctcttttcaa atgacatgcc
     4861 agttcagcgg tttgcgtttg tccaaccgat gtagccccgc ggcatgtgcc atatccactg
     4921 cagcctgata ttcctgctgg gtggtgcggc gtttaagctt ggggaatcgc tgagcattgt
     4981 atgctgggcg gtattgctcc atcaggttca ggtacgtgtc ggtagagatt tgctctgcca
     5041 ggaattgcac gacattctcg gtgccagcca ggttatgagg gagcactagg tggcgaacca
     5101 ataaaccccg ggttgcgaga ccgttctggt ccaggtgcaa gtcacctacc tggcggtgca
     5161 tctcgcgcac agcagcctga ttggcttggg ggtaattggg aatcttggaa taatagcgag
     5221 cgatctgcgc actagcgtat ttcatatctg gcatgtaaat atcgaccaca ccatccaata
     5281 gctttagcat ttcgacggaa tcatagccac cggtgttgta gacgagaggg atacgcaacc
     5341 cagcctgagc agcgatgagc accgcagcca tgatttgtgg tacaacatgg ctgggtgaga
     5401 ccagattgat attgtggcaa cccagggatt gtaattccag catgatggct gccagttgcc
     5461 ccggttctat ctcttcccct ttgtcggttt ggctgatatc atggttctgg cagaattgac
     5521 agcgcaggtt gcagcgcgtg aagaaaatag ttcctgaccc acgccaacca cgcaatggat
     5581 cttcctcacc tagatgcgga ccatagctgc tgaccttggc gcgctgggca gttttgcaga
     5641 tgcccacttt cccgactagg cgatctatcc tacactccca ggcgcatacg ttgcatttgg
     5701 agagcatatc ataagcatac gaaacgcgct gcgccagttc attgttgttc agtagtttga
     5761 cataagccgg ttcgtacatc aatatgcctg attattttct attgaaatct tatagcttct
     5821 ggcaaattgc ctggtttctg actataattt tgatgctgcc agttccttca agtatttcac
     5881 tgctttggct tcatattatt ttacatgcat tttaaacagg cgaacgttct aatcgttctt
     5941 ttcattttcg taatctcaac ctctgcatgc ggaatgttag gggcaaacaa tgcattagaa
     6001 gcatccgcta tcgatgatgc cgatccccaa tttcaggatt ttta
//
LOCUS       DBNGSX010000329         4971 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_373396, whole genome shotgun sequence.
ACCESSION   DBNGSX010000329 DBNGSX010000000
VERSION     DBNGSX010000329.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4971)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4971
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_373396"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..102)
                     /locus_tag="AC16CB_09960"
     tRNA            complement(15..102)
                     /locus_tag="AC16CB_09960"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66..68),aa:Ser,seq:cga)
     gene            complement(169..1566)
                     /locus_tag="AC16CB_09965"
     CDS             complement(169..1566)
                     /locus_tag="AC16CB_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3342834.1"
                     /translation="MKITFHGAARTVTGSRHLLQVNGATLLLDCGLFQGRRQESYQRN
                     LHLGFDPAKIDAVILSHAHIDHSGNLPNLVKSGFRGTIYATPATAHLGNLMLMDSGHI
                     QELDAAYVNKKRLRRGEPTIEPIYTMEDAAAVAPYFQPVNYKQRIEPIPGVTAHLVDA
                     GHILGSASVALEIEENGRKTRLWFSGDIGRRDLPLVRDPTLPEETDTLIMECTYGDKI
                     HRGPQEAYEQLREVVVRTVQRGGKVIIPAFAVGRTQELVYDLHRMIDSGEVPRIPVFV
                     DSPLAINASEIFRAHPECFDEETRQFIQEDKHQAVFGFDLLSYTRTVEESKALNERRD
                     PMVIISASGMAENGRILHHLANNIENPRNTVLIVSWQAPYTLGRRLAEKQEEVRIFGD
                     TFRRRAEVATIGGLSAHAGQNLLLEYAQRVKGSAKNIYLVHGEEKPAAVLTQLLAKDG
                     MSQVHYPQQGWSVEI"
     gene            complement(1625..2764)
                     /locus_tag="AC16CB_09970"
     CDS             complement(1625..2764)
                     /locus_tag="AC16CB_09970"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:NF013755.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HIR3342835.1"
                     /translation="MSSSEHLPAPIFIARPAALERLARLLQQEAIIAVDTESNSLYAY
                     QEQVCLIQFSTLSHDYLVDPLALKDLSSLGPIFSDPQIEKVFHAAEYDLICLRRDFGF
                     EFASIFDTMIAARILGRAEVGLGAILEAEFRVRLDKRYQRANWGRRPIPPELLAYAQL
                     DTHYLIRLRERLRRDLKEKSFWPLACEDFQRMGTTNGRPAENKGDDFWRINGARDLAP
                     QNAAVLKELWRYRDHMARVTNRPLFKVIGDRTLLAIAERCPNEAGELRQIPGMTSGQM
                     ARHGRELLAAVQKGLRAKPLYPPHLNRPDDKHLERFESLRNWRKTTARHMGVPSDVVL
                     PRDLMLLLAELNPRKPGDLSKIMEGVPWRLEHFGEQILAVLLSAQ"
     gene            complement(2769..3239)
                     /locus_tag="AC16CB_09975"
     CDS             complement(2769..3239)
                     /locus_tag="AC16CB_09975"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3342836.1"
                     /translation="MSTVVSAVPGCSTMILSATWRDFNIIRQIEHICFPRDAWPFWDI
                     FSVLTLPNVVRLKAVCNSQTVGFIASDVRPVEKMAWIATICVLPEFRQRGIGTALLHA
                     CEGRINLPSMRLNVRASNETAVRLYKNLGYSQIGVWPRYYQDGEDALILEKRLY"
     gene            complement(3249..4154)
                     /locus_tag="AC16CB_09980"
     CDS             complement(3249..4154)
                     /locus_tag="AC16CB_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011850007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR3342837.1"
                     /translation="MDIFGPGGTALCLVGAVIVIALAFVANAIRIVPEFQRLVVFRLG
                     RCIGARGPGIVFLIPIVDRAVRTDLREQVREIPHQTSITKDNAPISIDFLWYYKVLDP
                     TESVLQVGNFEAAAQGMATTTLRAVIGGIFLDEVLSEREHINNMLRTRLDDVTERWGV
                     KVTNVEIREIIPPREVQDAMNRQMSAERIRRAVVTESTGTREAAVNVAEGEKQAAILR
                     AEGSKQSAILEAEGARQAQLLKAEGFSAALEKIYSVAQTVDPKTMTLQYFETLKSIGA
                     SPSTKYIFPMEFSSLLGDFLKSRGE"
     gene            complement(4221..4829)
                     /locus_tag="AC16CB_09985"
     CDS             complement(4221..4829)
                     /locus_tag="AC16CB_09985"
                     /inference="COORDINATES: protein motif:HMM:NF051402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR3342838.1"
                     /translation="MNDVDILLNPNLAYLFLVLGFLLILMAIMTPGTGLLEIGGLILL
                     ILAGWEITNLPINAWALGVLLLAAIFFLLAFRQSRPQLYLGLSILALVLGSTFLFRGE
                     TWSQPAVNPFLALTVSVLSSGFIWLAARKTLEARRISPSHSLESLIGAIGEAKTEIFE
                     DGSIQVAGELWSARSEKTIPAGSQVRVIGREGFILEVEAINQ"
BASE COUNT         1022 a         1414 c         1429 g         1106 t
ORIGIN      
        1 gcgaatgaag agagcggaga gggtgggatt cgaacccacg gtggtcgcaa ggaccacaac
       61 ggttttcgag accgccccaa tcggccgctc tggcacctct ccaatcaacg tgtcacgtgg
      121 cacgggaaca cttgacacgg acttaccgcg attatagcgt agatcggcct aaatttccac
      181 gctccatcct tgctgtggat aatgcacctg gctcatgccg tctttcgcca gtagctgagt
      241 caacacagcc gcgggcttct cctcaccatg caccaggtag atgttcttgg cgctgccttt
      301 cactctctgg gcgtattcta gcagtaggtt ttgcccggcg tgtgccgaca ggccgccgat
      361 ggtagccacc tccgccctgc gccggaaggt gtcgccgaag atgcgcacct cttcctgctt
      421 ctctgccagg cgtcgaccca aggtatacgg agcctgccag gagacgatca gcaccgtgtt
      481 gcgcggattt tcgatgttgt tcgccaggtg gtgcaggatg cgcccgtttt ccgccattcc
      541 cgatgctgag atgatcacca tcgggtcccg gcgctcgttc agggctttgg attcttccac
      601 ggtgcgggtg tagctgagca ggtcgaagcc gaagacagcc tggtgcttgt cctcctggat
      661 gaactggcgt gtctcctcgt cgaagcattc cgggtgggcg cggaagatct ccgaagcgtt
      721 gatcgccagc gggctgtcca cgaagaccgg gatgcgcggc acctcgccgc tgtcgatcat
      781 gcggtgcagg tcgtacacca gctcctgggt gcgtcccacg gcaaaggctg gtatgatcac
      841 tttgccgccg cgctgcaccg tgcgcactac cacctcgcgc agctgctcgt aggcctcctg
      901 cgggccgcgg tggattttat cgccgtaggt gcattccatg atcagcgtat cggtctcctc
      961 gggcagggtc gggtcgcgca ccagcggcaa atcgcgccgt ccaatatccc cggagaacca
     1021 caggcgagtc ttgcgcccgt tctcttcgat ttccagggct acagatgccg agcccaagat
     1081 gtgcccggcg tccaccaggt gggctgtcac acctggaatt ggctcaatac gttgtttgta
     1141 atttaccggc tgaaaatatg gcgccactgc ggccgcatct tccatcgtat aaatcggctc
     1201 gatggtcggc tcgccgcgcc ttaatcgctt tttgttgaca taggccgcgt ccaactcctg
     1261 gatgtgacca gagtccatca gcatcaggtt acccaggtgc gcggtggctg gcgtggcgta
     1321 gatcgtgccg cggaagccgc tcttcaccag gttgggcagg ttgccgctat gatcgatatg
     1381 ggcgtgggac aagatgaccg catcaatttt tgctgggtcg aaccccaagt gcaggttgcg
     1441 ttggtaggac tcctgccggc gaccttgaaa caggccgcag tccaggagca aggtggcgcc
     1501 gttaacctgt agtaagtggc gggacccggt aaccgtgcgg gccgctccat gaaaggtgat
     1561 tttcataaga tttaggattt tcgcatttgg atggatggag taggtactgt ggactgaagc
     1621 acgtctattg agcggataaa agcaccgcta atatctgctc gccgaagtgc tccagcctcc
     1681 agggcactcc ctccatgatt ttgctaaggt cacccggctt gcgtgggttg agttctgcca
     1741 gtaacagcat caggtcgcgt ggcaatacca cgtccgatgg cacgcccatg tggcgcgcgg
     1801 tggttttgcg ccaattacgc agcgattcga agcgctccag gtgcttatcg tccgggcggt
     1861 tgagatgtgg cggatagaga ggcttcgccc gcaacccctt ttgcacggct gccagcagct
     1921 ctcgaccatg ccgcgccatc tggccgctgg tcattccagg tatctgtctc agctcacccg
     1981 cctcgtttgg gcaacgctct gcaatagcaa gcagcgtgcg gtcgccaatt accttgaata
     2041 gcgggcggtt tgtcacgcgg gccatatggt cacggtagcg ccatagctct ttcaacaccg
     2101 ctgcgttctg aggagccaaa tcgcgcgcgc cattgatgcg ccagaaatca tcgcctttgt
     2161 tttcagcggg gcgcccattt gtggtcccca tacgctggaa atcctcgcaa gccaggggcc
     2221 aaaaagactt ctccttcaag tcacgccgca ggcgttcccg caggcggata aggtagtgcg
     2281 tgtccagttg ggcataggct aataactccg ggggaatcgg ccgccttccc cagtttgccc
     2341 gctggtagcg cttgtccagg cgaacgcgga actcggcttc cagtattgcc ccaaggccca
     2401 cctcggcgcg ccctaggatg cgcgccgcaa tcatggtgtc gaagatgctg gcgaactcga
     2461 atccgaaatc cctgcgcagg cagatcaagt catactctgc ggcatggaag actttctcga
     2521 tttgcggatc gctgaaaatg gggcccaggc tggagagatc ctttaacgcc agcgggtcta
     2581 ccaggtaatc gtggctgagc gtggagaatt ggatcaaaca cacctgctct tgataggcat
     2641 acaggctgtt cgattcggtg tcgacggcga tgattgcctc ttgctgcaac agccttgcca
     2701 gcctctctag ggctgctgga cgagcgatga agatcggagc tggcaggtgt tctgaggaac
     2761 tcataaactt aatataacct tttctccagt atcaaagcgt cctctccgtc ctggtaatag
     2821 cgcggccaaa cgccgatttg ggaatacccc aggtttttat ataggcgtac agctgtctca
     2881 tttgaggcgc gcacatttag gcgcatgctg ggtaggttga tgcgaccctc gcaggcatgc
     2941 aacagtgccg tgccaatgcc tcgctggcgg aattccggca agacacagat ggtagcgatc
     3001 caggccatct tctccactgg acgaacgtca ctggcgatga atcccaccgt ctggctgttg
     3061 caaacagctt tcaagcgcac cacgtttggc agggttaata cgctgaaaat gtcccagaag
     3121 ggccaggcat cccgcgggaa gcaaatatgt tcgatttgac ggatgatgtt aaagtcgcgc
     3181 caggtggccg acaggatcat cgtcgagcaa cctggcacgg cagatacaac ggtgctcatc
     3241 aggcgggttt attctcctcg gctcttgaga aaatcaccca agaggctgga aaactccatc
     3301 gggaaaatat atttggttga tgggctggcg ccgattgact tcagagtttc gaagtattgc
     3361 agcgtcattg tcttggggtc caccgtttgg gctacagagt agattttttc cagggcggcg
     3421 gagaagccct cagccttcag caattgtgcc tgccgagcgc cttctgcctc aaggatggct
     3481 gactgcttcg agccctctgc tcgcaaaata gccgcctgct tttcaccctc agccacgttt
     3541 accgcggcct cgcgcgtgcc cgtcgattcg gtcaccacgg cgcggcggat gcgctcagca
     3601 gacatctggc ggttcatcgc atcttgcact tcacgcggtg ggatgatctc gcggatctcc
     3661 acattggtga ccttcactcc ccagcgctcg gtgacgtcat ccaggcgagt gcgcagcatg
     3721 ttgttaatgt gctcacgctc tgacagcact tcatccagga aaataccgcc gatgaccgcc
     3781 cgcagggtgg tggttgccat accctgggca gccgcttcaa agttgcccac ttgcaggacg
     3841 ctctcggttg ggtcaagcac tttgtagtac cacaagaaat cgatcgatat cggggcgttg
     3901 tctttggtga tcgatgtctg gtgagggatt tcacgcactt gctcgcgcaa gtcggtcctc
     3961 actgcccggt ctacgatcgg gatgaggaag acgatccccg gcccgcgcgc cccaatgcag
     4021 cgacccagcc ggaagacgac caggcgctgg aattcgggaa cgatgcggat ggcgttggca
     4081 acaaaagcca gggcaatgac gataactgct ccaaccaggc atagagcagt ccctccgggt
     4141 ccaaatatgt ccatgattga cctccagtgt ttttaggttg agacccaaaa agtctcaggg
     4201 accttttggg gtttcgatgt ttattggttg atcgcctcta cttccagaat aaagccctcc
     4261 cgcccgatga cgcgaacctg agagccagcg gggatggtct tttcgctgcg ggcagaccac
     4321 agctcaccgg ctacctgaat ggaaccatct tcgaaaatct ccgttttggc ttcaccaatg
     4381 gccccaatca agctctctag actgtgagag ggagatatgc gccgggcttc aagcgttttg
     4441 cgtgcagcaa gccagataaa cccgctgctg agcacggata cggtcagcgc caggaaaggg
     4501 tttactgctg gctggctcca agtctcccca cgaaatagaa acgtggagcc aagcaccagg
     4561 gccaagatag acaaccccag atacagctga ggtctggatt ggcgaaatgc cagcaggaag
     4621 aaaatggctg ccagcagcaa gacacccagc gcccaggcgt tgattggaag gttggtaatt
     4681 tcccagccgg ccaaaataag cagaatgagt ccaccaatct cgagcagacc ggttccgggg
     4741 gtcattattg ccatgaggat tagcaaaaag cccagcacca ggaataagta tgccagattg
     4801 gggttgagca atatgtcaac gtcattcata tgctcattat acatcaacat gtcccggaga
     4861 atcatgagcg tggtatcatt ggcactgtga ttgcattcac cctgccaggc ggcttgtcca
     4921 ttgtttcttt ctctttatct ttggggatag gggcagtaat tggtctcgcc t
//
LOCUS       DBNGSX010000330         2304 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_997714, whole genome shotgun sequence.
ACCESSION   DBNGSX010000330 DBNGSX010000000
VERSION     DBNGSX010000330.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_997714"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(298..1116)
                     /gene="pheA"
                     /locus_tag="AC16CB_09990"
     CDS             complement(298..1116)
                     /gene="pheA"
                     /locus_tag="AC16CB_09990"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIR3342839.1"
                     /translation="MRVAFQGEPGAYSEAAIFEHFGTQVKTVPCETFDQVFESVASEA
                     SQRGLIPVENSLAGSIHRNYDLLLQNSLTVVGEHHLHVHHCLIALPGVALSEIQRVIS
                     HPQALAQCDATIRSLGHVKAEAAYDTAGSVKIVSQQNNRTLAAIASRQAAELYGMQIL
                     AENIEDNPANITRFQIIACQPVNPGADAKTSIVFSLKNEPGSLFKALSVFALRDIDLT
                     KIESRPLAGKPWEYFFYIDFAGSTQDEVVSRALSHLEEYATFLRVLGSYPRHRV"
     gene            complement(1123..2082)
                     /locus_tag="AC16CB_09995"
     CDS             complement(1123..2082)
                     /locus_tag="AC16CB_09995"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 -
                     branched-chain-amino-acid:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HIR3342840.1"
                     /translation="MGMESKYLWMDGKLVEYEKATIHFMTPALHYGVAAFEGVRSYAT
                     DNGPAIFRAKEHAARLRRSAKVLGFRELPYTEAELVEAMKLTVAANQFVECYIRPLIY
                     LTGGGWNLSVDGGKASLGISVWEWNNYLGDEALDKGIRANITSFTRHHPNVMMTKAKI
                     SGNYANSVLARTESMRLGFDEAIMLDPQGYVAECTGENLFLVRDGKIITPPTAAILEG
                     ITRDSIITLAKELGYEVIEQPISRDQLYVADEVFVCGTAAECIGLWEIDYRVIGNGKS
                     GPVTKDIQKAFHAAIRGQHPRSAEWLDYVNTAVPAFLPVDAMD"
BASE COUNT          547 a          632 c          590 g          535 t
ORIGIN      
        1 aacaagcgag aaaatgggta aaaggcttat aatgatagga taaaagctaa atctttttag
       61 tagagtatcc aattcttaaa ttccttcaat attcttgttc gtcaaagccg tgaggcattc
      121 aaataaattt cattcaggcg aagattaaac ctcaatttac caaggactcc ttatcccgtt
      181 taatagtgga tatgcgatat gtagtgatag cagggatatc tttagaacaa ttggttaaac
      241 gttattccac taagtacact gggaaacaga atacctccca tcctgagatc aatttattca
      301 tacacggtgg cgcggatatg agcctaaaac gcgcaggaaa gtagcgtact cctccagatg
      361 ggacaaagcg cgggagacaa cctcatcctg agttgaaccc gcaaaatcaa tgtagaagaa
      421 atactcccag ggtttgccag ccagcggtcg agattcgatc ttggtcaggt cgatgtcgcg
      481 cagcgcaaag acgctcagcg ccttgaacaa agagccaggc tcatttttca gagagaagac
      541 gatagatgtt ttcgcatccg ctccgggatt cactggctgg caagcgatga tttgaaagcg
      601 cgtgatgttg gcaggattat cttcgatatt ttcagccagg atttgcatcc cgtatagctc
      661 cgctgcctgg cgggaagcga tcgccgccaa ggtgcggtta ttttgctggc ttacgatctt
      721 gacgctgcca gccgtgtcat aggcggcttc tgctttaacg tgacccagcg agcgaatcgt
      781 ggcgtcacat tgggccagag cctgggggtg actgatcacc ctctggattt cggatagcgc
      841 gacccccggc agggcgatca agcagtgatg gacatgcagg tggtgctccc ccaccaccgt
      901 cagcgagttc tgcagcagca ggtcgtaatt gcggtggatg ctccccgcca gcgagttctc
      961 gacagggatc aagccgcgct ggctagcttc cgaagcaacc gattcaaata cctgatcgaa
     1021 ggtctcgcac ggaactgtct ttacttgagt cccaaaatgc tcgaagatgg ctgcctccga
     1081 ataggctccg ggctcgcctt gaaaggctac acgcatacct tgtcaatcca ttgcgtcgac
     1141 tggaaggaag gccggcacag ccgtattcac ataatccagc cactccgccg aacgcggatg
     1201 ctggccgcgg atggcggcat ggaacgcctt ttggatatct ttggtgaccg ggccggattt
     1261 tccattacct atcacgcggt aatcaatctc ccacagcccg atgcactcgg cagcggtgcc
     1321 gcacacgaac acttcatccg caacatatag ctggtcgcgc gagatgggtt gttcaatcac
     1381 ctcatagccc agctccttag ccagggtgat gatcgaatcg cgcgtgatgc cctccaggat
     1441 ggcggctgtg ggtggggtaa taatttttcc atcccgcacc aagaacaggt tttcgcccgt
     1501 gcattcggct acgtatccct gcgggtccag catgatcgcc tcgtcaaatc ccaggcgcat
     1561 ggattcggtg cgcgccagca cgctattggc atagttaccg ctgattttgg ccttggtcat
     1621 catcacgttg gggtggtggc gggtaaacga ggtgatattg gcgcgaatac ccttatccag
     1681 ggcttcgtcg cccagatagt tgttccattc ccacacagag atacccaggc tggcttttcc
     1741 gccgtccacg gataggttcc aacccccacc ggtcagatag atcagtgggc ggatatagca
     1801 ctccacgaac tggttagcag ccaccgtcag cttcatcgcc tccaccagct cagcttcggt
     1861 atatggcaac tcacgaaagc ccaggacctt agcagatctc cgcagccgcg ccgcatgttc
     1921 cttggcgcgg aatatcgccg gcccattatc ggtggcataa ctgcgcacgc cctcgaaggc
     1981 tgccacaccg taatgcagcg ccggggtcat aaagtggata gtggctttct cgtactcaac
     2041 cagcttgcca tccatccaga ggtattttga ttccatgccc atcgcttgtt tctcctttag
     2101 ataaaaatta gagcttaatc gttgaaggaa aagtctcatc aacgcggatg atcaccttcg
     2161 agatcatcca tcggcagcaa gacgctgcac aatctcgcta gtcatctggg tagtggtcat
     2221 cgacccaccc aagtcagccg tacggcaacc gtcagccaat acgcgattaa cagccttctc
     2281 gattgcggcg gcttcgcctt ccaa
//
LOCUS       DBNGSX010000331         3018 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_997888, whole genome shotgun sequence.
ACCESSION   DBNGSX010000331 DBNGSX010000000
VERSION     DBNGSX010000331.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_997888"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(305..1189)
                     /locus_tag="AC16CB_10000"
     CDS             complement(305..1189)
                     /locus_tag="AC16CB_10000"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022834.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxamate carbamoyltransferase subunit AllH family
                     protein"
                     /protein_id="HIR3342841.1"
                     /translation="MSQKTITISEIGAIARGLLAQESNASVMGVTSRGLFLLLNSGWV
                     VFLSPEAYRGPLTLNYRSHTDPFRLLQTKQSAKIAAGSISIPDAGLLINTGQADTWEA
                     PSIPGGILPPEQRAAQLVNIARHLLGIKQTSQLSELLPLLLGWVDRAEFQDNSLLPLL
                     DDVKRSLHRRQATEIAHSLVALLGMGSGLTPSGDDVAIGFLLAMARWGHELAPDLDIG
                     ALGQMILPQAYRKTTTLSANMIDCALRGQANERLLLALDGILSGNAHASACSDLLAGW
                     GNTSGLDALLGISLAIGV"
     gene            1674..2570
                     /locus_tag="AC16CB_10005"
     CDS             1674..2570
                     /locus_tag="AC16CB_10005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3342842.1"
                     /translation="MTLNAHPELPEFDYIRPASLMEASRFLAQHPGEARPLLGGTDIF
                     VRMRDGFWHDKYLVDVKNLDSTHDLHFDPASGLTIGAGVNMNRVIASPEVQAHYPLLA
                     EACRSVASYQLRTRATIVGNLCNASPAGDTIGACLLLDGALKVHGLDGLREVPLNTFF
                     LGPGKTVLKAGDIVTALKIPLPANGCAGIYLKLGRNKLSDLAIVGITAYGYPDATTTS
                     GYRMRVALASVAPVPLVVPAIEKYLMDNPINPEAFQHAGNLAQEACNPIDDVRGSARY
                     RKAMVRRLTTRALLNIWENIKS"
     gene            2650..>3018
                     /locus_tag="AC16CB_10010"
     CDS             2650..>3018
                     /locus_tag="AC16CB_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3342843.1"
                     /translation="MSFHRISMMVNGEYEQIDVPSNMTLMQMLRDKLALTGTKNGCNA
                     GECGACTVLLNGEPVNSCMVLAVECEGANIVTVEGLAKDGVLDPVQQTIIDHGGVQCG
                     FCTPGILISSRALLDRIPNPT"
BASE COUNT          747 a          793 c          760 g          718 t
ORIGIN      
        1 gatcaggacg cgaaagtctt gcatcctctg ctcgcatagg tcatctagca agcggggcaa
       61 tgtactctca ccgtctaggg gtgtgggatg gtcgtaggag gctgtctccg ggcgatggct
      121 cgcgctttgc ggccatgtcc agtaggtagg gataataaca ctgacattct tcataatcga
      181 gttccgtaca cccttttata cccaagtttc ccagtaattg caactttttt ggctcacaga
      241 ggaccagcga gttcaacacg aaccgcagat tcacccgtga cgacttgtgt ggctagggaa
      301 tacgctaaac cccaatagcc agggagattc ccaacagggc gtccagccct gaagtattcc
      361 cccacccagc caggagatca gagcaggcgg atgcatgtgc attaccgctc aggatgccat
      421 ccagcgccag cagcaggcgc tcatttgcct gaccccgcaa ggcacaatcg atcatgttgg
      481 ctgacagcgt cgtagtcttt cggtacgcct gcggcaggat catttgaccg agggcaccaa
      541 tgtccagatc aggagcgagc tcatgccccc agcgtgccat cgctagcaaa aagccgatcg
      601 ctacatcatc ccccgagggg gtcagaccac tacccatccc cagcaaagcc accaggctat
      661 gcgctatctc agttgcttgg cgacgatgga gcgaacgctt cacatcatcc aggagcggta
      721 agagcgaatt atcttggaat tccgctctat caacccatcc caaaagaagt ggcaataact
      781 cactgagctg gctggtctgt tttatgccaa gcaagtggcg ggcgatgttc actagctgag
      841 cggcgcgttg ctcgggaggc aggatacccc ccggtatgga aggcgcctcc caggtgtcag
      901 cctgcccggt atttatcagc aagcctgcat ctggaataga gatactgcct gcagctattt
      961 tggctgattg tttggtctgc agtaatctaa aagggtctgt atgactgcga tagttgagag
     1021 tcagtggtcc tcgataagcc tcaggcgata ggaaaaccac ccatcctgaa tttagcagca
     1081 ggaacaagcc acgcgaggtg acacccatca cactggcatt gctctcttga gccagcaatc
     1141 ctcgggcaat ggctccgatt tcactgatgg taattgtttt ttgagacata actgagcgtg
     1201 ttttaattaa ccgttagctt gcgcctgagt tccgaatata tcaatgagca tcccaaatct
     1261 attactctta acgtactctt tatcgaagag atcctccttg acaaatatca tatcgagaag
     1321 atgacatcta ccagttgaac ggcatatcaa ttgccacgat aataataaca gtgtttacgc
     1381 ggttgggatc taactaggtg gtatgggcgt gctttactgt ccataccacc aattttctat
     1441 tagactatgg atgctcattt tgtatcccgt ggtaactttg acagatatag ggtgttttac
     1501 cattggcaga ggaggtcctg agttaattaa ataaatgagc cttccaacct aaaatcgcat
     1561 tccatcccac ctagcaaatt gaactggatt tcatgatttt gcccgcagcc agccacgaag
     1621 aatgacccag ccattgatag aatcacagtc cttaaataga tgaggtgtga aagatgaccc
     1681 ttaatgctca cccagaactt cctgaattcg actatatccg gcctgcatct ttgatggaag
     1741 caagccgttt cttagcccag catcctggcg aagcgcgccc gcttttaggc ggcacagaca
     1801 tattcgtccg catgcgagat ggattctggc acgataaata cctggtcgat gttaagaact
     1861 tggatagcac gcacgatctt cactttgacc ccgcttcagg gttgaccatc ggcgctgggg
     1921 ttaatatgaa ccgggtgatc gcttcccccg aggttcaagc acactacccg ttactcgccg
     1981 aagcctgccg ctcagtagca tcttaccaat tgcgcactcg ggctactatc gtgggcaatc
     2041 tctgcaacgc gtcacctgct ggggacacga tcggcgcatg cttgttacta gacggggcac
     2101 tgaaagtaca tggcctcgat ggcttgcgcg aggtcccctt gaatacattc tttcttgggc
     2161 ctggtaaaac cgtgctgaaa gctggcgata tcgttacagc cctaaaaatc ccgctgcctg
     2221 caaatggttg tgctggtatt tacctcaagc tgggacgaaa caaattgagt gacctcgcta
     2281 tcgtcggcat aaccgcctat ggctatccgg atgcgacaac aacctctggg tatcgcatgc
     2341 gtgtggcgct ggcctcggtt gcgcccgtcc cactggttgt ccctgcaatc gagaaatacc
     2401 ttatggataa ccccatcaac cctgaagcat tccaacatgc tggaaatctg gcacaagaag
     2461 cctgcaaccc gattgatgac gtgcgcggca gcgcccgcta tcgcaaagcg atggtgcgca
     2521 gactgacgac aagagcgctg ctaaatatct gggaaaacat caaatcgtag cgcgtattga
     2581 atatttggcg gtcaagacaa tctatcggca agagatattt ataacgagct gcgcattctg
     2641 gaggtaataa tgtcatttca tcgaatatct atgatggtca atggtgagta cgagcaaatt
     2701 gatgttcctt ccaatatgac tctaatgcaa atgctgcggg ataaattggc gctgaccggc
     2761 accaagaacg gatgcaacgc cggtgagtgc ggcgcctgca cggtgcttct gaacggtgag
     2821 cccgtcaaca gttgcatggt gctggcagtt gagtgtgagg gagcaaatat cgtcactgta
     2881 gaaggcttgg caaaggacgg cgtgctcgat ccagtacaac agaccatcat cgaccacggc
     2941 ggggttcaat gtggcttctg cacgccagga attctcattt cgtcgcgagc gttgctggat
     3001 cgtattccca accccacc
//
LOCUS       DBNGSX010000332         6292 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_789740, whole genome shotgun sequence.
ACCESSION   DBNGSX010000332 DBNGSX010000000
VERSION     DBNGSX010000332.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6292)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6292)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6292
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_789740"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="AC16CB_10015"
     CDS             complement(<1..702)
                     /locus_tag="AC16CB_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342844.1"
                     /translation="MSKPIIGVGLTGELSLRVSVATLSRVVFPHPEGGVSMLALEHKA
                     TLAAGEADMVDVKAQPFGGAIRILNPNRLLTLLGSFNFDSQRSRSEQDFRVYIRPSSW
                     EEVREFCLQNMDQEAGPDLEIDPSRELEEEFEDALGIQLKLNQYVVKPFGIVVENEPA
                     PTTNVRAAGNLTARIYRVYEVQIQDPALWRLMVTNSQTHPKQILQRLALDDSQKGGRG
                     RATAMLVAAEQEIRAA"
     gene            complement(708..4166)
                     /locus_tag="AC16CB_10020"
     CDS             complement(708..4166)
                     /locus_tag="AC16CB_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3342845.1"
                     /translation="MPESVLSFQSLPQEYQQVIQLAEDRYGIAIAPLQELAGGWSGAI
                     IYLVRVASTDSKKVEHLILKLDRKRTMSSSDEVSRHKKVQQISPSEFTNLHIPEMAYD
                     QVEAENALAIFYTIAGQSLHNFLTLSSHQRQSHIETLFSATNHILLDSWNAGLTFEQI
                     DHPRDLLQKWLGFRLDAGQNIEIFLSEVCQLPPNIPGFIVQGNILPNPLYYARNIDAW
                     TSVRAGDAMVGLQHSDLNTNNILAKFSRQGDELEGYYLIDFALFKENMPLLYDQRYLE
                     MSYLTHTISQGIYNSVVGLICRYGEQDILEPDQTAIEMTGVNAAIRAGRIAFGDWVRE
                     NHPSLHDDLWGQYWLAGTAAGLSYCHKAGQADGIRLAGLIYASANLKQYFKLFGLPMP
                     TGASQLYAESQIGGMPAAGFTLASPARKPNHNLPAPPTNFIGRTAELAELTDLLLRPE
                     VRLVSLTGPGGTGKTRLSLEAGRALLDRFPQGVYFVDLAQINDPSLVSATTAHTIGIR
                     EGGGRPPLETLKDYLAGRGMLLIFDNFEQVVGAAADISQLLAAAPGIKALVTSRTPLH
                     LRGEHEYPVSPLDMPPDAYQSIDQAIKYEAVALFRQQARTVQPAFDLTEDNRAAVIEI
                     CRRLDGLPLAIEIAAARIKMLSPQALLDRLDHSLSLLVGGAKDLPNRQQTLRGTIDWS
                     YELLQPVEQILFTRLGIFAGGFTLNAADEICKPMGEVDVFSGVETLLNNSLLRQVHSA
                     SSEPRFDMLQTIREYALEKAAEAGIMTELQWAHCGYYSRMAAAAGNMGEGSFGAHSVI
                     WLQRYEEEYDNFRVALSWGIKHPEEGFPLVIAMMNQLAWFWYRYGYLQEGSEWTERSL
                     AATQGMGDSPARAWALVGRAYLALWSGDLLIAARYGREAVEMCQRISFDLGLSLAKLT
                     FGTTLINQGKDREAYPHLVDAVELYDQQDQPWMKGTALVHLANVSLGLGDPEQATQWL
                     DMAMPFLLNSGDVWAMAFGLNNYGEVARAQGDYDRAEDFYRRTDELYKQADAKGDQAR
                     LLHTFGYIAQHKGDLNGARSLFLESLNDFRKLGNHRGIAECLAGLAGLGAIQGKHEWA
                     TPLLSSAESQLKAIGGAWWPADRVEIERARERLRAALDDRFAELWAQGQSMGVEEAIA
                     YATNEA"
     gene            complement(5070..5303)
                     /locus_tag="AC16CB_10025"
     CDS             complement(5070..5303)
                     /locus_tag="AC16CB_10025"
                     /inference="COORDINATES: protein motif:HMM:NF020553.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HIR3342846.1"
                     /translation="MEITPKTKVSDILEEYGDIADVMEIFGIKRVARYSFRKMLTKAL
                     TVEVAARVHRVPLDEFLETLNNAIEMRAKNQEP"
     gene            complement(5315..5695)
                     /locus_tag="AC16CB_10030"
     CDS             complement(5315..5695)
                     /locus_tag="AC16CB_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342847.1"
                     /translation="MNLKNMFATSAIVAILLGLGLILVPVPLMTARTGHPPDVLTAHV
                     ARQFGIAMLAAGVISWMARRTTDSTARNAIVVGLALLYVLLPIETAISIVRGSESAEG
                     FVLVVVFGILAVGFILLGKPRLTF"
BASE COUNT         1491 a         1706 c         1558 g         1537 t
ORIGIN      
        1 ggcggcgcga atctcctgct ccgctgccac cagcatggcg gtcgccctgc ctcgcccgcc
       61 tttttgagaa tcgtctaacg ccaatctttg caagatctgt ttgggatgtg tctggctgtt
      121 agtcaccatc aatcgccata aggcaggatc ttggatctgc acttcgtaaa cccggtagat
      181 ccgggcggtt aggttgccag cagcacgcac gttggttgtg ggggccggtt cattttccac
      241 cacgatgccg aacggcttca caacgtattg gtttaacttg agctggatcc ccagcgcatc
      301 ctcaaactct tcctccaatt ccctagaagg atctatttcc aggtctggac ccgcttcctg
      361 atccatattc tgcaggcaga attcgcgcac ttcttcccag ctcgaggggc ggatatagac
      421 ccggaaatct tgctcggaac gcgatcgttg gctgtcgaag ttgaagctgc ccaggagtgt
      481 caaaagccgg ttgggattta atattcggat agccccccca aacggctgtg ctttcacgtc
      541 taccatgtca gcctctccag cagccagggt agctttatgc tccagcgcga gcatcgagac
      601 tccgccctct ggatggggga agacgacccg cgaaagcgtg gccacgctga cccgcaagct
      661 cagttcacct gtgaggccga cgccgatgat tggcttactc acagcgatta cgcctcattc
      721 gtggcgtagg caatcgcctc ttcaacaccc atagattggc cttgcgccca gagctcagcg
      781 aagcggtcgt cgagagcggc tcgcagacgc tctctcgccc gctcaatttc tacccggtct
      841 gcaggccacc aggcgccgcc aatcgcctta agctggctct ccgcgctgct caacaaaggt
      901 gtggcccact catgttttcc ttgtatggct cccagaccag ccaggccagc caggcattct
      961 gcgataccgc gatggttgcc caattttcga aaatcattga ggctctccaa gaataacgag
     1021 cgtgccccat taagatcacc cttatgctgg gcaatgtagc cgaatgtgtg tagaagacgt
     1081 gcctggtcgc cttttgcatc cgcctgcttg tacaattcgt cggttcgccg gtagaaatct
     1141 tctgctctat catagtcccc ctgggctcga gcaacttctc cgtagttatt caatccgaaa
     1201 gccattgccc atacatcacc agaattcaac aagaatggca tggccatatc caaccattgg
     1261 gtggcctgct ctggatcgcc taatcctagt gacacattgg ctaggtggac caaggctgtt
     1321 cctttcatcc agggttgatc ctgctggtca tagagctcga ccgcatccac caggtgggga
     1381 taggcttctc tgtccttgcc ttgattgatc aatgtcgtcc cgaaggttag tttagccagg
     1441 gataagccaa ggtcaaagct tatccgttgg cacatttcca cagcttcccg cccatatcgc
     1501 gcggcaatca acaggtcgcc tgaccacaaa gccagatatg cgcgcccgac tagcgcccag
     1561 gcgcgcgccg gcgaatcacc catcccttgt gtagccgcca ggctgcgttc ggtccattca
     1621 ctgccctctt gcaagtaacc atagcggtac cagaaccagg ccagctgatt catcatcgca
     1681 atcaccagtg ggaagccttc ttcaggatgc tttatacccc aggagagggc cacacggaaa
     1741 ttatcatatt cttcttcata gcgctgcagc caaatcacag aatgggcgcc aaaactccct
     1801 tcgcccatat ttcctgcagc cgccgccatt cgtgaataat agccacagtg agcccattga
     1861 agctcggtca ttatgccggc ttctgctgct ttttccaatg cgtactcgcg aatcgtctga
     1921 agcatgtcga agcgtggctc actgctggcc gaatggacct ggcgaagcaa gctgttattt
     1981 agtagcgtct caacaccaga aaaaacgtcc acttcgccca tcggtttgca gatttcatct
     2041 gcagcattca gggtgaaccc cccggcaaag attcccaatc gtgtgaagag gatctgttcc
     2101 acaggttgga gcaattcata actccagtcg atggtcccgc gcaaggtttg ctgccggttg
     2161 ggtaaatcct tggctcctcc caccagcagg ctcaagctgt ggtccaggcg atcaagtaat
     2221 gcctgggggg agagcatctt gatgcgggcg gcggcgatct cgatcgccag gggaagacca
     2281 tcaaggcggc ggcagatctc tatgaccgca gcacggttgt cctctgtaag atcgaatgct
     2341 ggctggacag tcctggcttg ctgccgaaaa agagccaccg cttcatattt aatagcctgg
     2401 tcaatagatt ggtatgcatc cgggggcatg tccagagggg agactgggta ttcatgctcc
     2461 ccgcgcaggt gcaaaggggt tcgactggtg accaacgctt tgatcccagg agcagcggcg
     2521 agcagctgcg agatgtccgc ggcagctcca acgacttgtt cgaaattatc gaaaatcagg
     2581 agcattcctc tgccagccag gtagtctttc aaagtctcta atggcggtcg acctccgcct
     2641 tcacggatcc cgatcgtgtg agcggtggtt gccgaaacca gtgacggatc gttgatttgc
     2701 gccaggtcaa cgaaatacac accttgggga aagcggtcca gcaaagctct gcctgcttcc
     2761 aggctcaggc gggtttttcc cgtcccaccc ggccctgtca acgaaaccag ccgcacctcc
     2821 ggacgaagca gcaaatcggt taactcagcc agttcagccg tccggccgat gaagttcgtc
     2881 ggcggtgctg gcaaattatg gttcggtttt ctagccgggg aagccagcgt gaagcctgct
     2941 gcgggcatgc ccccgatctg actttcggcg tacaactggc tggcaccggt gggcatggga
     3001 agaccaaata gcttaaaata ctgcttcagg ttggcggagg cataaatcag ccccgccagt
     3061 cgtattccat cggcctgacc cgctttgtgg cagtagctca accctgccgc tgttcctgca
     3121 agccagtatt gaccccacaa gtcgtcatgc aagctgggat ggttttcccg tacccagtct
     3181 ccaaaggcga tccgccctgc acgtatggct gcattgacac cagtcatttc gatcgctgtt
     3241 tggtcgggct ccaggatgtc ctgctcccca tagcggcaga tcaggcctac cacgctgtta
     3301 tagatcccct gcgagatcgt atgggtcaga taagacatct ccaggtagcg ttgatcatac
     3361 agtagtggca tgttctcttt gaagagggca aaatcgatca ggtagtagcc ttctaattca
     3421 tccccctgtc gcgaaaactt ggccaggata ttgttcgtgt ttaaatcgct gtgctgcagc
     3481 ccgaccatag catccccagc cctgactgag gtccaggcat cgatatttcg cgcatagtac
     3541 agcggatttg ggaggatatt accctggacg atgaaacccg ggatattggg gggcaactga
     3601 cagacctcgc ttaagaaaat ctcaatattc tgacccgcat ccagacgaaa ccccagccac
     3661 ttctgcagca gatcccgggg gtgatcaatc tgctcaaatg tcagacctgc attccagcta
     3721 tccagcaata tgtggttggt cgctgagaac aaggtttcaa tgtggctctg gcgctggtgg
     3781 cttgagagcg tgaggaaatt gtgcagcgat tggccagcta tcgtataaaa aatcgccagg
     3841 gcattctcag cttcaacctg atcgtaagcc atttcgggga tgtgcagatt ggtgaactca
     3901 gagggggaaa tctgctgtac ctttttatgt cgggagactt catcggaaga cgacatcgtc
     3961 cgtttgcggt caagcttcag gattaggtgt tccacttttt tggaatctgt ggaagcgacg
     4021 cgcaccaggt aaatgattgc tcccgaccac ccaccggcca attcctggag cggggcaatg
     4081 gcgatcccat atcgatcttc cgcgagctga ataacttgtt ggtattcctg gggcagcgat
     4141 tgaaaactca gtacagattc cggcatagtt cttacctttc aaacccagaa tgtgcggtag
     4201 taaataacag cttggaggat tatagcatta catttctctc cactccccag acgaccattt
     4261 gaatagggca atcgcatttg ggcgattgcc caccactgag gagagaaaag aatgcttacc
     4321 accctatcac cgttctggat cacccattat cgaaacatcc gtaccacaaa caaaaactgc
     4381 tcgacaacat cttgcggttc ttcgtgtaac ccgtagcgca tgagttccga accacaccca
     4441 tgcctctttc taagggtaat aagttacgaa cgtgttacat tttcaagaat cttggtctca
     4501 aaatcacgag tcgtcaaatt atgctggcgt tatctttttt gaattattta ctaccgaaat
     4561 ttgtgattgc caaataatca accgtactct gcaactcatt caaaaaccca ggaagatgaa
     4621 agccatcaat aatgccaata tctttcactg cttcaagcaa tgtggaaata tcgatgttta
     4681 ctgtcatgct gaatatgttt ggtactttat tgaagtggac atcaaagtat taaccacgag
     4741 gccaagtttt ccaatcgatt ggtgtaaatt tcataaggtt tccttaaata gcttacagtt
     4801 cggcaatgct ttctgcagct tgccaacccc ggtatcggca accttcgtga aacggaggtt
     4861 tagcttgcgg aggttaacca gcccggcaag gtgcgtaaaa cctgtgttca cattgggcgc
     4921 tcaggtgtag gttcgaactg gtcgaggagg gcgaccgcgc cagtctggac tggttcggtg
     4981 gatatggcaa aggtgcctgt ccttgatgaa gaatgcttag ccgctaggtt gtcccccaca
     5041 tgtgatctag agatcgcttc ggcgccgtct catggctctt gattcttcgc acgcatttca
     5101 atagcgttat tcagtgtttc caggaattca tcaagtggga ccctgtgcac acgcgcagcc
     5161 acctcaaccg tcaaagcttt ggttagcatc tttcggaatg agtagcgcgc cacgcgcttg
     5221 atgccaaata tctccatcac atcagcgata tcaccgtatt cttccaggat atcggatact
     5281 tttgttttgg gtgtgatttc cattggttgt ctctttagaa ggtcaatctt ggtttgccta
     5341 acagtatgaa accgacagcc agtatcccaa aaacgactac caatacgaag ccttcggcgc
     5401 tttctgagcc acgcacaatt gagatggcag tctcgatggg taacagcacg tatagcaagg
     5461 ctaagccaac tacaatagcg ttgcgagcag tcgaatcagt tgtgcggcgc gccatccaag
     5521 agattactcc tgcggctagc atggcaatac caaactgcct ggcaacatga gcggtcagaa
     5581 catcaggggg atgaccagtc cttgcagtca ttaacggcac cggtaccagg ataagtccca
     5641 gtccaagcag gatcgcgaca attgcgctgg tggcaaacat attcttcagg ttcataactc
     5701 gttctccagt tttcgaaatt aagagggata aagcttggtc tgacacgatt ggatactggt
     5761 cgtcagcagg tgatctaaaa tatgttgaca gcctgaacta tctggataat ctgttatcca
     5821 tctttgagaa agtcccgcaa ttatacttca taaaaatatt tactaaccat gcttcataaa
     5881 tgccctggtt gattacaact tcatgacgct tgtaaagttt ctgctccttg atccaaaacc
     5941 actgcatcat ccgtttgtgg tcgtcgatga aatgcccaat gcgcaactgc tgtaagtcct
     6001 aaatgcgaag cgctgtattg ataccaacca ctatcagtaa atgatcacgg tagcgctttt
     6061 tccgcaggct taccccccac atataggtga cccgtagcgg atgagttccg aaccaccccc
     6121 ttccctagaa ttaggagaaa acacagtgct aagtgaggaa tccatatata ggtctgctaa
     6181 gtcacaagat gaacatcagc cctgtagaga ccagctgatt tggaataact caatcatcta
     6241 gatcgtagat cggaaatcta tctgtggtgc gggaaaaaac aggtctctgg tc
//
LOCUS       DBNGSX010000333         7902 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1482190, whole genome shotgun sequence.
ACCESSION   DBNGSX010000333 DBNGSX010000000
VERSION     DBNGSX010000333.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7902)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7902)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7902
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1482190"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="AC16CB_10035"
     CDS             <1..1203
                     /locus_tag="AC16CB_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR3342848.1"
                     /translation="IRRCFACHTDHQGEDFDPTQAAFALFDHSKTRFSLLRHQVNYDA
                     SPMECLACHQYEGKMAFSDSYCTLCHAAENMDFAVQHSQQYGANCSYCHDGGDRLADF
                     DHQTTAFPLEGKHAQAYCADCHAPQELAVGRGGGGLSSSQPVGTPSDCVECHAKDDPH
                     PEMFTSDCKACHTPDAWSPATWEEAPFEHAIQAGFSLSLHRVDYEGNPLSCLGCHTGD
                     AQSFEQESCISCHSQGDERAAFMVEHQAQYGSDCVACHDGVDRMANFNHEAVFPLDGR
                     HAEIACQECHADQVFTGTPATCAECHAEPDIHAGFFGLECQYCHSAQAWTPALLIQHR
                     FPLDHGEQGEITCNACHPDSYIEYTCYGCHDHQPDETAAKHQEEGISLEKLADCASCH
                     PAGLKEDN"
     gene            1219..3459
                     /locus_tag="AC16CB_10040"
     CDS             1219..3459
                     /locus_tag="AC16CB_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342849.1"
                     /translation="MRKFLLFSIPIALALAMLVSSMGAYLAYAAPFQPGSSLFPLQEF
                     AEEKGAFLFINPTGKANYYLDLSERRVMDLLELARTDREVIALKALDNALDQAEIAIA
                     AAPDEDLPALMARLANVVTQIEKLVSVLTIAPIEDPDTVAALLAQVETLRSLLAGSGA
                     STAQSLENARKLLVLSVVGTHEDPSTLDVSAIAAIDPRIINFPAGSLGAQHTFFPLGG
                     GHAGLACESCHKNGQFSGTSSQCIDCHRNVVPANHYPGDCASCHTILAWDDIHFNHQV
                     VATKDCQSCHARPKNHFSGQCSACHSTQAWKPARFNHESTGAKDCQSCHTRPKNHFSG
                     QCSNCHSTSAWRPAKFDHTGLTDCQSCHKRPSNHFSGQCSACHSTSAWRPAKFDHTGL
                     TDCQSCHKRPSNHFSGQCSTCHSTSAWKPAKFDHTGLTDCQSCHKRPSNHFSGQCSAC
                     HNSSAWRPAKFDHTGLTDCQSCHSRPKNHFSGQCSTCHNSSAWRPAKFDHTGLTDCQS
                     CHSRPRNHFSGQCSACHTTSAWKPANFNHTGLTDCQSCHNRPSNHYSGQCSNCHNTSG
                     WSSVRFSHAGLTDCQSCHNRPSNHYSGQCSNCHNTSGWGSASFSHTGLTDCQSCHNRP
                     SNHYSGQCSNCHNTNGWGSVSFSHSGLTDCQSCHNRPSNHYSGQCSDCHNTQNWSDVR
                     LSNHTFPINHKGANGRCSVCHPSGGSSYTCYNCHDRSKTEEHHNEKGITDIASRCADC
                     HPRGEE"
     gene            3456..3737
                     /locus_tag="AC16CB_10045"
     CDS             3456..3737
                     /locus_tag="AC16CB_10045"
                     /inference="COORDINATES: protein motif:HMM:NF024634.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3342850.1"
                     /translation="MNITSLFKLEITRRKALVQGYVVPEPARCVQCGVCTYNCPINID
                     VRQHAWKGIPVKDSHCLTCGECVARCPRGVLRFEGTSMFTSQTPGLAGD"
     gene            3739..5061
                     /locus_tag="AC16CB_10050"
     CDS             3739..5061
                     /locus_tag="AC16CB_10050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3342851.1"
                     /translation="MRRYVMIGSGVASIAAIEAIRSVDPRGEITLIGDESHGYYSRPG
                     LAYYLNGEINERQLFPMDQANYHKLNIRRLHARVERIYPDQHLVRLHNGATMPYDRLL
                     IAVGAEANMMNIPGVDLHGVVKLDKLDDARWIIKLARKARTAVVVGGGITALEIVEGL
                     VKHGVRTHYFLRGDRYWSNVLDKTESQIVENRLRGTGVHIHYHTELREILGKNGRVVG
                     VRTQDNQTMRCDILAIAIGIRPRKELAEISGLCVDRGVEVSETLQSSAPDVFAAGDVA
                     QVYDPFTGKSVVDSLWGPARDQGHTAGLNMAGITTPYYKLIAFNVTRLADLTTTIVGT
                     VGRGDDQDVIGIVRGDSETWRQLPDAIAVQSHFEINRLRLQIGKNNLLGAIIMGDQTL
                     SRPLHQLITEQADITPIRQQLLQPGAPLVDILADFWCHWKARYAFATQ"
     gene            5048..5866
                     /locus_tag="AC16CB_10055"
     CDS             5048..5866
                     /locus_tag="AC16CB_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342852.1"
                     /translation="MRRSNRELWLSFAAIVCISLAYLFVLAWQDGIPRASEFFGHSLG
                     ILGFIMMVMTETLYTLRKRSHSARWGRMADWLDFHIFTGIVGPYMVLLHSSWKFNGLA
                     GIVMLLTVTIVASGFIGRYIYTSVPRTVDGVEVEARLLEEQIAAADQELKNWLSAPDK
                     SLRNLAHNFSTAPLGSAGQFTLIVGRGLLDLTYQLNWWLKRRRLSSQERQQARVLEKL
                     LSRRRTLEIQLASLTTARRLLALWHAVHIPIGMVLFTAAIIHIIAAIYYATLLR"
     gene            complement(5872..6315)
                     /locus_tag="AC16CB_10060"
     CDS             complement(5872..6315)
                     /locus_tag="AC16CB_10060"
                     /inference="COORDINATES: protein motif:HMM:NF016408.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR3342853.1"
                     /translation="MFRRREDPLLPPTQPMPTERVTSVLGSGIVWKGNLSGVGGIRIE
                     GAFEGDISLRGMLVIGETGRVTCEHLRANVVIVAGAVQGNITAEKVEIRRTGRVWGDV
                     TTAAFATEEGAFLRGQIRMEEQVELGTGASSEADAEAESEAGVDG"
     gene            complement(6318..7100)
                     /locus_tag="AC16CB_10065"
     CDS             complement(6318..7100)
                     /locus_tag="AC16CB_10065"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0030497 - fatty acid elongation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HIR3342854.1"
                     /translation="MKLLEGKTALVFGLANERSIAWGITKAFHEHGATLGISYAGEVL
                     ERRVRPLAAEIGCNFVESCDVTADEQIEMVAQKAAEAFGQIDILVHAIAFANRDELSG
                     PYYNTSRAGFHTAMDISVYSFTALAKAFQPLLRPGGALLTLTYYGAEKVSPHYNVMGV
                     AKAALEASVRYLAYDFGKQKVRVNAISAGPIRTLAAAGVSGFKSMYRQFAEIAPLHEN
                     ITIEDVGGAAVFLCSDLAGKTTGEVTYVDSGYNILGIPEPVD"
     gene            complement(7205..7810)
                     /locus_tag="AC16CB_10070"
     CDS             complement(7205..7810)
                     /locus_tag="AC16CB_10070"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HIR3342855.1"
                     /translation="MPDPEQLERPFFSRSTLQVARELIGMRLVRQERNVRLVGVISEV
                     EAYRGEEDQGCHARAGRTPRTRVMYGSPGHAYVYFTYGMHWMLNFVTEAEGYPAAVLI
                     RAILPVEGLDVIAARRDGQPFAHWTDGPAKLCQALGIDGRYNGLDLCAPQSEIFVQVG
                     ESIPDSAVTIGPRVGLNNVPEPWKSIAWRFRIQSADFTGVY"
BASE COUNT         1856 a         2363 c         1995 g         1688 t
ORIGIN      
        1 atcaggcgtt gttttgcttg ccataccgat caccaaggag aagatttcga ccctacacaa
       61 gcggcatttg ccttgttcga tcactcgaaa acgcggttca gcctgcttcg tcaccaggtc
      121 aattatgacg catcgccaat ggagtgcctc gcctgtcacc agtatgaagg gaagatggcg
      181 ttttcggatt catattgcac tttgtgccac gctgcggaaa atatggactt tgccgtccag
      241 cacagccagc aatacggtgc aaactgctca tattgccatg atggaggaga ccgcctggct
      301 gacttcgacc accaaacgac tgctttcccc ctggaaggaa aacatgcaca ggcttactgt
      361 gccgattgcc acgcgccgca ggaactcgct gttgggcgcg gcggtggagg cttatcctca
      421 agtcagcctg tgggcacgcc aagtgattgc gtcgaatgtc atgccaagga tgatccccac
      481 cctgagatgt tcacatccga ttgcaaagcc tgccataccc ccgatgcctg gtctcctgcc
      541 acttgggaag aggcaccctt cgagcacgcc atccaggcgg gcttcagcct atccttacac
      601 cgtgtggatt atgagggcaa tccactctcc tgcctgggtt gccacacagg agatgcccag
      661 agctttgaac aggaaagctg tatatcctgc cattctcaag gggatgaaag ggctgctttc
      721 atggtcgagc accaggcaca atatggtagc gactgtgtcg cctgccatga cggcgtggat
      781 cgaatggcca atttcaacca cgaggccgtc ttccctctgg acgggcgcca cgccgagatc
      841 gcctgccagg aatgtcatgc tgatcaggtc ttcaccggca cgccggccac ttgtgcagag
      901 tgtcacgccg agccagacat ccatgccggg ttctttgggc tggaatgcca gtattgccac
      961 agcgcccaag cctggacacc tgccctgttg atacaacacc gctttccctt ggatcacggc
     1021 gaacaaggtg aaattacctg caatgcctgc cacccagaca gttatatcga atacacctgc
     1081 tacgggtgcc atgaccacca gccagatgaa actgctgcaa agcaccagga agaaggaatc
     1141 tcgctcgaaa agcttgcgga ctgcgccagt tgccacccag ctggtctcaa agaggataat
     1201 tagcctgagg aaaacgccat gcgaaaattc ctgctgttct cgatacccat tgcacttgcg
     1261 ctagctatgc ttgtgtcgag tatgggagcg tacctggctt atgccgctcc cttccaacct
     1321 ggcagttccc tttttccact gcaggagttc gccgaggaaa aaggcgcctt cctatttatc
     1381 aaccctaccg gcaaggcgaa ttactactta gacctgagtg agcggcgagt aatggatctt
     1441 ctcgagttag cacgcacaga tcgggaggtt atcgcactga aggccctgga taatgccctg
     1501 gaccaggcag aaatagctat cgccgcggcg cccgacgaag atctcccagc gttgatggcg
     1561 cgcctagcga atgtggtgac acagatcgaa aagctcgtct ccgtgctaac catcgcccca
     1621 attgaagacc ctgatactgt ggcagcgctt ttagcccagg tggagacgtt gcgctcgctg
     1681 ctggctggat ctggggcgtc taccgctcaa tcactggaga acgccaggaa gctgctcgtt
     1741 ctttctgtgg ttggcacgca tgaagaccca tccacattgg atgtctccgc tatcgccgcg
     1801 attgacccaa gaatcatcaa cttcccagcc ggcagcctcg gggcgcaaca caccttcttc
     1861 cccctaggtg gagggcacgc tggtttagca tgtgaaagct gccataaaaa cgggcaattc
     1921 tccggtactt ccagccaatg tatcgattgc catcgcaatg tggtgcctgc caaccattac
     1981 cctggcgatt gtgcttcctg tcatacaatc ctggcatggg acgatatcca cttcaatcac
     2041 caggtagtgg caaccaagga ctgccagtcc tgccatgctc ggccaaaaaa ccacttcagc
     2101 gggcagtgct ctgcctgcca tagcacccag gcatggaaac cggcgcgttt taaccatgag
     2161 tccacaggcg caaaagattg ccagtcttgc catacaaggc cgaaaaacca ctttagcggg
     2221 cagtgctcca attgtcacag caccagcgca tggagaccag ccaaatttga ccacaccgga
     2281 ctgaccgact gccagtcttg tcacaaaagg ccgtcgaacc acttcagcgg gcagtgctct
     2341 gcctgtcaca gcacaagcgc atggagacca gccaaatttg atcacactgg cctaacagac
     2401 tgccagtctt gtcacaaaag gccgtcgaac cacttcagcg ggcagtgttc tacctgtcac
     2461 agcaccagtg catggaaacc agccaaattt gatcacaccg gactgaccga ctgccagtct
     2521 tgtcacaaaa ggccgtcgaa ccacttcagc gggcagtgct cggcatgcca caattccagc
     2581 gcatggagac cggccaaatt tgaccacact ggcctgaccg attgccagtc ctgccactca
     2641 agaccgaaaa accacttcag cgggcagtgc tctacctgtc acaattccag cgcatggaga
     2701 ccggccaaat ttgaccacac aggtctgaca gattgccagt cttgccattc aaggccgaga
     2761 aaccacttca gcgggcagtg ctcggcatgc cacactacca gcgcctggaa gccagctaac
     2821 ttcaaccata ctggcctgac cgattgccag tcttgccata accgcccatc caaccactac
     2881 tccgggcagt gctcaaactg ccacaacact agtgggtgga gctcagtgag gttcagccac
     2941 gccggcctga ctgactgcca gtcctgccac aaccgcccat ccaaccacta ctccgggcag
     3001 tgctcaaact gccacaacac cagtggatgg ggatcagcga gtttcagcca tactggtctg
     3061 actgactgcc agtcctgcca caaccgacca tccaaccatt actccgggca gtgctcaaac
     3121 tgccacaaca ccaatggatg ggggtcggtg agtttcagtc actccggctt gaccgattgc
     3181 cagtcctgcc acaatcgccc gtccaaccat tactccgggc agtgctccga ctgccataac
     3241 acccaaaact ggagcgatgt gcgcctaagt aatcacacct tccccattaa tcataaaggt
     3301 gcaaatggtc gctgttcggt ctgtcaccct tcaggcggaa gttcatatac atgctacaac
     3361 tgtcacgacc ggagtaaaac ggaagaacat cacaatgaaa aaggcatcac cgatatcgct
     3421 tcccgttgcg cagattgcca tcctcgaggt gaggaatgaa catcaccagt ctatttaaac
     3481 tcgaaatcac ccggcgcaag gccctggtcc agggatacgt agttcccgaa ccggcccgct
     3541 gtgtgcagtg cggtgtctgt acatataact gcccaatcaa cattgatgtg cgccagcatg
     3601 cctggaaagg gatcccggtg aaggacagcc actgcctgac gtgcggcgaa tgtgttgccc
     3661 gttgtccgcg cggtgttctt cgcttcgagg gtaccagcat gttcacatca cagacgcccg
     3721 gattggctgg agactgagat gcgccgctac gtgatgatcg gcagcggtgt ggcttccatc
     3781 gcagcgattg aagccatccg cagcgtcgat ccccgtggag agatcacctt gattggggat
     3841 gaatctcacg ggtactattc gcgtccaggt ttagcctatt acctgaacgg tgagatcaat
     3901 gagcggcaac tttttcctat ggatcaagcc aactatcata agctaaatat ccgccgtctg
     3961 cacgcacggg tagaacgaat ttatccagac caacatctcg tccgcctaca caatggagcc
     4021 acaatgccct acgaccgcct gttgatcgct gtgggagccg aggcaaacat gatgaatatc
     4081 cctggcgtgg acttacacgg cgtggtcaaa ctagacaagc tggacgacgc ccgctggatc
     4141 atcaagctgg ctcgcaaagc gcgaacggcg gtcgttgtgg gcgggggcat caccgcattg
     4201 gagattgtcg aagggctggt aaaacatggt gtgagaacac attacttctt gcgcggcgat
     4261 cgttactgga gcaatgtgtt ggataaaacc gaatcacaaa ttgtagaaaa ccgtctgcga
     4321 ggaactggag tgcatatcca ctaccacacc gaactcagag agatcctagg caaaaacggg
     4381 cgtgtggtgg gcgtgcgcac ccaagacaac cagacgatgc ggtgtgacat actagctatt
     4441 gcaattggta tccgtccccg caaagagctg gcggagatat ctggcttgtg cgtcgatcgc
     4501 ggcgtcgagg tgagcgagac cttgcaaagc agtgctcccg acgtgttcgc cgcgggtgat
     4561 gtagcccagg tgtatgaccc cttcactggc aagtcggtag tggacagcct ttggggacca
     4621 gcgcgagacc aaggacacac tgccggatta aacatggcag gtatcaccac tccatactat
     4681 aagctaattg catttaacgt gacccgactg gccgatttga ccacaaccat cgtcggcaca
     4741 gtcggtcgag gcgacgatca ggacgtgatc ggcatcgtcc gcggcgatag cgagacctgg
     4801 cgccagcttc ccgatgccat cgccgtccaa tcccacttcg agatcaaccg cctgcgcctc
     4861 cagataggta aaaacaactt attgggtgcg atcattatgg gcgaccagac tctctccaga
     4921 ccattacacc aattgatcac cgaacaggcg gatatcacgc cgatccgcca gcagcttctc
     4981 cagccgggtg cgccgctggt ggatatcctg gctgatttct ggtgccattg gaaagcgaga
     5041 tacgcctttg cgacgcagta accgcgagct gtggctctca tttgctgcca tcgtatgcat
     5101 cagcctggcg tacttgttcg ttctggcctg gcaggatggc atcccgcgcg ccagcgaatt
     5161 ctttggtcac agcctcggca tcctgggttt tattatgatg gtaatgaccg agacgctcta
     5221 cactttacga aagcgcagcc atagcgcacg ctgggggcgc atggcagact ggctggactt
     5281 ccacatcttc accgggattg tcggtccata tatggtgctg ttgcactcct catggaagtt
     5341 taatggcctg gctgggatcg tgatgctgct cactgtcaca atcgtcgcca gcggctttat
     5401 tggtcgctat atctatacct ccgtgccgcg taccgttgac ggcgtggagg tagaagcgcg
     5461 cctgctcgaa gagcaaattg ctgccgctga ccaggagcta aaaaactggc tcagcgcgcc
     5521 agataagtct ctgcgcaacc tggcacataa tttctccacc gcgccactgg gttctgcagg
     5581 gcagttcacc ttaattgtgg gcagaggctt attagatttg acctatcaat taaattggtg
     5641 gctaaagcgc cggcgcttgt cctcgcagga acgccagcaa gcccgggttc tggagaaatt
     5701 gctatctcga aggagaacac tggagatcca gctggcatca ctaaccacgg cacgtcgtct
     5761 gctggcgctg tggcacgcgg tgcatatccc cattggcatg gtactcttca ccgctgccat
     5821 catccacatc atcgcagcaa tttattacgc cacgctcttg cgataatcga tctatccgtc
     5881 aaccccggct tcggattcag cttctgcatc tgcttccgag cttgccccag tccccaattc
     5941 tacctgctct tccatgcgga tctgaccgcg tagaaatgcc ccctcttcag tggcaaatgc
     6001 cgcagtagtt acatcccccc atacacgtcc agtcctacgg atctccactt tttcggcagt
     6061 gatgttaccc tgcactgctc cagccacaat gaccacatta gcgcgcaggt gctcgcaggt
     6121 gacccgcccg gtctccccta tgaccaacat accccgcagg gaaatatcgc cttcgaaggc
     6181 gccttcaatt cggatcccac ccacaccgct caggttgcct ttccacacaa tgccgctgcc
     6241 caacaccgag gtaaccctct ccgttggcat gggttgggtg gggggaagta aaggatcctc
     6301 ccttctacga aacatcttta atcaaccggc tccgggatgc ccagaatgtt ataccccgaa
     6361 tcgacatacg ttacctcgcc cgtggtctta cctgccaggt cggagcacag gaataccgcc
     6421 gcaccgccca catcttcaat ggtgatattt tcatgtagag gagcgatctc agcaaactgg
     6481 cggtacatgc tcttaaaccc agatacgcca gctgccgcca gtgtgcggat tgggcctgcg
     6541 gagatcgcgt ttacacgcac cttctgcttg ccaaagtcat acgccaggta gcgcaccgag
     6601 gcttccaaag ccgccttggc gacacccatc acattatagt gaggggaaac tttctccgcc
     6661 ccataatatg tcagcgtaag cagggcgcca ccggggcgca ataagggctg gaacgcttta
     6721 gccaatgctg tgaaggaata cacgctgata tccatcgccg tgtgaaaacc agcccggctg
     6781 gtattgtaat acggcccgct cagctcatcc cggtttgcaa atgcgatggc atgcaccagg
     6841 atatcgatct gcccaaaggc ttccgcagcc ttttgtgcca ccatctcgat ctgctcgtcg
     6901 gcagtcacgt cacaggattc tacaaagtta caaccgatct ccgccgcaag ggggcggaca
     6961 cgccgctcca agacttcgcc tgcgtagctg atgcccaagg tggcgccatg ctcatgaaaa
     7021 gccttggtaa tcccccaagc aatcgaacgt tcattggcta gaccaaatac cagcgcggtt
     7081 ttaccttcaa gtaatttcat ctattcctcc acaatcaatc attacagacg caggatataa
     7141 gtattatgct ccaaatacag ttttactgcg ggttaacacc aatggttaaa ccaatatctc
     7201 cgctttaata aacaccagta aaatctgcag attgtattct aaaacgccat gcaatacttt
     7261 tccagggttc tggcacatta tttaacccta cccgagggcc gatggttacg gctgaatcgg
     7321 ggatagattc gccgacttgg acaaaaatct ctgattgtgg agcgcacagg tccaggccgt
     7381 tataccgccc atcgatgccc aacgcctggc acagcttagc tggcccatcg gtccaatggg
     7441 cgaagggttg cccatcccgc cgcgcggcga tcacatccaa cccttccact ggcaagatgg
     7501 cacgaatcag caccgccgcc ggataaccct cagcctcagt tacaaagttg agcatccagt
     7561 gcatcccata ggtaaagtac acataagcgt gacccgggga accgtacatc acgcgcgttc
     7621 tgggagtccg gccagcgcgc gcatggcagc cttgatcctc ttcaccacgg taagcttcta
     7681 cctcgctaat cacacccacc aaacgcacgt tcctttcctg ccgtaccagg cgcatgccaa
     7741 tcaattcccg cgccacttgt aaggtgcttc gggagaaaaa aggtcgttct aattgctcgg
     7801 ggtcgggcat aaattagcgc aggggtcgag taggctcttt cttaaaacgc tgatcgcgct
     7861 ccatagtcat gctgagtcca tctaccagtg aaacgcgtgg ct
//
LOCUS       DBNGSX010000334         4989 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_789900, whole genome shotgun sequence.
ACCESSION   DBNGSX010000334 DBNGSX010000000
VERSION     DBNGSX010000334.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_789900"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="AC16CB_10075"
     CDS             <1..329
                     /locus_tag="AC16CB_10075"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HIR3342856.1"
                     /translation="IWGVMTLEYWLALRFLGIQVDLLQAVAALTAARIAFLFPLPGGL
                     GALEASQILAMEALGLNPALGLSISLLIRGRDITLGGLGILLGGIMTRRRARVVVPNQ
                     ATERRI"
     gene            347..679
                     /locus_tag="AC16CB_10080"
     CDS             347..679
                     /locus_tag="AC16CB_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342857.1"
                     /translation="MNTGQTKFGAMQIGIILLALATAVIHIILAIPENMVMFYLNGVG
                     YLALTAALYLPQFEGMRGKVRWALIGYTAVTVIGWVAFGERNPVAYIDKIIELALIAL
                     LWVEGRQK"
     gene            684..1328
                     /gene="ribA"
                     /locus_tag="AC16CB_10085"
     CDS             684..1328
                     /gene="ribA"
                     /locus_tag="AC16CB_10085"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020481345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HIR3342858.1"
                     /translation="MDSLIVEHKACARIPTDMGEFQLNLYITNQDSKEHLALVMGEVR
                     DQSDVLVRVHSECFTGDVLGSRRCDCGEQLQHSMQMIAATGAGVLIYLRQEGRGIGLM
                     DKLRAYNLQDQGYDTVDANLALGHQADERDYSVAAGILDDLGVRSIRLLTNNPVKIQH
                     LQDLGVPVLERVPLHSTVNPDNAQYLFTKALRMNHLLDLEAFPVGATNRKNGSH"
     gene            1315..2013
                     /locus_tag="AC16CB_10090"
     CDS             1315..2013
                     /locus_tag="AC16CB_10090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HIR3342859.1"
                     /translation="MVPIDELLAGAQKHRHNTGRPLVTLSYAQSLDGSITAERGIPLR
                     LSSPASMEMTHHLRAAHDGILVGIGTLLADDPRLNVRLVQGRDPQPVVLDSRLRFPLD
                     ANLLKGNRPPWIASLPGTDPQKRVELEKHGATILSLAEGGDGRVSLPDLLTCLADKGL
                     NSLMVEGGASVITSFFAQGLADRLVLTIAPVIIGGLRAVEPEASLASLPRLENAGFER
                     LGGDMIVWGALSYP"
     gene            2032..3048
                     /locus_tag="AC16CB_10095"
     CDS             2032..3048
                     /locus_tag="AC16CB_10095"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR3342860.1"
                     /translation="MQRQSVIFSAPGQVAICDEPLADPAPNQVLVKTLCSAISPGTEL
                     LVYRGQIPEQLPLDVTIPSLAQEARYPLRYGYSCVGQVVAFGSQVERQWEGRHVFAFQ
                     PHTSYFLAEPQELMPLPTGIEPENAIFLPNMETAINFCMDGAPLIGERLAVFGQGIVG
                     LLTVMLLAQYPIHNLVTLDCYPLRRKVSIEIGAHASLDATAGDVLEQLRGIQPGGADL
                     VYELSGSPIALDQAIAATVFSGRVVVGSWYGKKRVELDLGGHFHRNRIRLLSSQVSTI
                     APELSGRWTKKRRFELAWEMIRRLQPKRFVTQYIALNDAPAAYHLLDQQPEETIQVVL
                     DYPR"
     gene            3129..3521
                     /locus_tag="AC16CB_10100"
     CDS             3129..3521
                     /locus_tag="AC16CB_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342861.1"
                     /translation="MAVEEAFQMTVQIALEDWDDEEVHSLAVQLAGELGDQPVESVEL
                     ARAGAAPVGTKSPEAITLGAVAVAVLPSVLPKVVEFCQAWALRGQGRTVKFEGKIHGQ
                     DIKFEGTADELQRLLAQLSSPPPAGNPA"
     gene            3548..>4989
                     /locus_tag="AC16CB_10105"
     CDS             3548..>4989
                     /locus_tag="AC16CB_10105"
                     /inference="COORDINATES: protein motif:HMM:NF047832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HIR3342862.1"
                     /translation="MARRYALVIGNSQYEDTTLSQLKTPEADVHALASALRDPQIGGF
                     DEVQEMINQSESAVRRAISAFFLQKKSDDLLLLYFSGHGVLDPQGRLFLAVKDSQRNL
                     LNATAIPANFITDDMDSCRSKRQILILDCCHSGAFAKGAKGDAPAVTQSTFEGNGYGR
                     VVLTASDSTQYALEGDQVIEQASFSLFTHYLLEGLISGEADIGLDGLITLDEWYDYAY
                     DRVVGETPEQTPRKWVYNQQGELVIARNPNPPQLGPEDLPEVLKLAIQSPFVAVRADA
                     VEELKRLLLGSNPAISQAAFQALENLAAHDDSKRIASAAGDALAALRQPAQPDRSEAE
                     HQEVEKVDQERQVREKAHVEPQKAEKADAERQDSAIDSQERLAEEAPISQQVTYLPFS
                     SELRPQKVKAGEDAEVLIEVSGQDTVSFSVDFVDPSGELDFAPPAAQLTVQPGYNAAV
                     KFQAGLRQRPLFKLNPALPYKAQVAASTGEA"
BASE COUNT         1055 a         1342 c         1474 g         1118 t
ORIGIN      
        1 tgatctgggg cgtgatgacc ctggagtatt ggctggcttt gcgcttcctg ggcatccagg
       61 tagacttgct ccaggcagtt gccgccctga ccgctgcccg cattgctttc ttgttcccat
      121 tgccgggggg gttgggtgcg ctggaggcta gccagatact tgcgatggaa gcccttggac
      181 tcaacccggc tttgggtttg agcatcagcc tgctcatccg ggggcgggac atcacattag
      241 gtggtttagg tattttgtta ggcggcatca tgacgcgccg gcgcgctcgt gttgtcgtgc
      301 ctaaccaggc cacagagaga agaatataac taaggaggaa tataaaatga acacaggaca
      361 aaccaaattt ggggcgatgc agattggaat tattctgctt gctctagcaa ccgcggttat
      421 tcatatcatc ctggcaatac ctgagaacat ggtgatgttt tacttgaatg gcgtcgggta
      481 tttagcattg actgctgccc tatacttacc tcagtttgag gggatgcgtg gtaaggtgcg
      541 ctgggcactg ataggctaca ctgctgtaac ggtgatcggc tgggttgcat ttggggagcg
      601 aaacccggtg gcttatatcg acaaaattat tgaattagcg ctgattgcct tgttgtgggt
      661 cgaaggacgc caaaagtagt atcatggata gcttgattgt tgaacacaaa gcttgcgccc
      721 gcattccaac ggatatgggc gagttccagc tcaatttata catcactaac caggatagca
      781 aagaacactt ggctttggtg atgggagaag tgcgcgatca gagcgatgtg cttgttcggg
      841 tgcactcgga atgtttcacc ggggatgtcc tcggttcgcg gcgctgcgac tgcggcgagc
      901 aattgcagca ctctatgcag atgatcgctg ctactggggc tggcgtgttg atctacctgc
      961 gccaagaggg gcgcggtatc ggcctgatgg ataagttgcg cgcctacaac ctgcaagacc
     1021 agggatacga cacggtggat gctaatctgg ctctgggtca ccaggccgat gagcgggatt
     1081 acagcgtggc ggcaggtatt ctggatgatt tgggcgtgcg ctccatccgc ttgctaacca
     1141 ataacccggt taagatacag cacctgcaag atttgggtgt gcctgtcttg gagcgcgtgc
     1201 cactgcattc cacggtcaac cccgacaacg cccagtacct gttcacgaaa gccttgcgta
     1261 tgaaccatct tcttgacctg gaagcctttc ctgtgggggc gacgaaccgt aagaatggtt
     1321 cccattgacg agttgctggc aggagcgcag aagcaccgcc acaatacagg tcgcccgctc
     1381 gtcacactga gttacgccca aagcctggat ggaagcatta cagcagagcg gggcattccc
     1441 ttgcgactaa gcagccctgc ctcgatggag atgacccatc atctgcgcgc ggctcatgac
     1501 ggcatcctgg tgggcattgg cacgctgctg gcagatgacc cccgcctcaa tgtgcgcctt
     1561 gtgcagggcc gcgacccgca gccagtggtg ctggatagcc gcctgcgttt tccccttgac
     1621 gccaacctat taaagggcaa ccgacccccg tggattgctt ccctgcccgg tacagatccg
     1681 caaaaacggg tagaattaga gaagcacggg gcgaccattc tgagcctggc ggaaggcggc
     1741 gatgggcgtg tctccttgcc tgatctgctt acatgcctgg ctgataaggg cttgaatagc
     1801 ttgatggtcg agggcggggc aagtgtcatt accagtttct ttgcacaagg attggctgac
     1861 cgactggtgc taacgattgc cccggtcatt attggcggtc tgcgtgctgt ggaacctgag
     1921 gcatccctgg cctctctccc ccgcttggag aacgcgggtt ttgagcgtct tggtggcgat
     1981 atgatcgtct ggggcgcact aagctatccg taaacaataa ttcatcatct catgcaacgc
     2041 caatctgtta ttttctccgc tcccgggcag gtcgcgatct gcgacgagcc gctggctgac
     2101 ccagctccaa accaggtgct cgtaaagacc ttgtgctcgg caataagccc gggcactgaa
     2161 ttgctggttt atcgcggcca aatccccgag cagttgccgc ttgatgtgac catcccatcc
     2221 ctggcgcagg aagcccgcta ccccctgcgc tatggctact cctgtgtagg acaggtggtg
     2281 gcttttgggt cgcaggtcga gcgccagtgg gaagggcgac atgttttcgc tttccagcct
     2341 cacaccagct attttctagc tgagcctcag gagctgatgc cgctacccac aggaattgag
     2401 ccagaaaatg cgatcttctt gcccaacatg gagacggcga tcaatttctg catggatggc
     2461 gccccgctga ttggcgagcg cctcgcagtc tttgggcagg ggattgtcgg tcttttgact
     2521 gtgatgttgc tggcgcaata ccccatccat aatttggtca cattggattg ttacccgctg
     2581 cggcgtaagg tctccataga gatcggggcg cacgccagcc tggatgctac agcgggtgat
     2641 gtactggaac agttgcgggg tatacaacct ggcggtgcgg atttggtcta tgagctatcc
     2701 ggctctccta ttgccctcga ccaggctatc gctgctacgg ttttctccgg gcgggtggta
     2761 gtcggctcct ggtatggaaa gaagcgggtc gagctggatc tgggcgggca ttttcatcgc
     2821 aaccgcattc gcctgctcag cagccaggtc agcacgatcg ccccggaact gagcggacgc
     2881 tggaccaaga agcgccgttt tgaattggcc tgggagatga tccgccgctt gcagccgaag
     2941 cgttttgtca ctcaatatat agctttgaat gacgctccgg cagcttatca cttgttggat
     3001 cagcagcctg aagagaccat ccaggtggtg ctggattacc cccgctgagg ttgccaattc
     3061 ggatctttta atgtaaacta attatatcta gacaatattg gtcatcatta taagaacggg
     3121 gattccgaat ggcagtagaa gaagcattcc aaatgacagt acaaattgcc ctggaagatt
     3181 gggacgatga agaagtgcac agccttgccg tccagctagc tggcgagctt ggtgatcagc
     3241 ctgtcgaatc ggttgagcta gctcgcgcag gcgctgcgcc tgtaggcacg aagtcgcccg
     3301 aagcgataac gctaggggca gtggcggtag ccgtgttgcc cagcgtattg cccaaggtgg
     3361 tggaattctg ccaagcctgg gcgctgcgcg gccaggggcg cacggttaag tttgagggca
     3421 aaatccacgg gcaggatatt aaatttgaag ggacggctga cgaactccag cgcctcctgg
     3481 cgcagctctc cagcccgcca cccgctggaa atccagccta gtctctctgg ttaaccgcgg
     3541 aggttgcatg gcgcggagat acgcacttgt tataggcaac agccagtacg aagacaccac
     3601 tctgtctcaa ctgaaaacac ccgaggccga tgtgcacgcc ctggcgtctg ccttacgcga
     3661 cccgcaaatc ggtggcttcg atgaagtcca ggagatgatc aaccagtctg agtccgctgt
     3721 gcgacgcgct attagcgcct ttttcctgca gaagaaatct gatgacttgc tcctgttgta
     3781 tttttccggc cacggtgtgc tggatcccca ggggcgcctg ttcctggcag tgaaggatag
     3841 ccagcgcaat ctgctcaatg ccactgcaat accggctaat ttcatcaccg atgatatgga
     3901 cagttgccgc tccaagcgcc aaatcctcat tctggactgc tgccatagtg gggcttttgc
     3961 caagggtgct aagggagatg ccccggctgt cacccaatct accttcgaag gcaatggata
     4021 tggcagagtt gtcctcaccg cctccgactc cacccagtac gcactggagg gtgaccaggt
     4081 tatcgagcaa gcctcgtttt cgctcttcac ccattacctg ctggaaggct tgatcagcgg
     4141 agaggcggat attggcctgg atggcttgat caccctcgac gagtggtacg attacgccta
     4201 cgatcgcgta gtgggggaga cgcctgagca gacccctcgt aagtgggtct acaaccagca
     4261 aggcgagctg gttatcgccc gcaaccccaa tcccccgcag ttaggccccg aggacctgcc
     4321 cgaggtgctc aagctggcta tccagagccc atttgtagcc gtgcgcgcgg acgccgtaga
     4381 ggagttgaag cgcctcttgc tcggcagcaa tccagcgatt tcgcaggccg ccttccaggc
     4441 tctggagaac ctggcagccc atgacgacag caagcggatt gccagcgcgg ccggggatgc
     4501 cctggcggca ttgcggcagc ctgctcagcc ggatcgatct gaagctgagc accaggaggt
     4561 agaaaaagtc gatcaggagc gccaggttag agaaaaggcg catgttgaac ctcaaaaagc
     4621 ggaaaaagcc gatgcagagc gtcaagatag cgcaatagac tcgcaagagc gcctggctga
     4681 ggaagcgccc atcagccaac aggttactta cctgcctttc agcagcgagt tgcgccccca
     4741 aaaagtcaaa gctggggagg atgctgaggt tttgatcgag gtttcaggcc aggatacggt
     4801 atctttctca gtggattttg tagatccgag cggggaattg gatttcgcgc cgcctgccgc
     4861 gcagctcact gtgcagccgg gctacaatgc ggcggtaaaa tttcaagccg ggcttcgtca
     4921 gcgccctctg ttcaagctaa atcctgcgct tccatataaa gctcaggtgg cggcatcgac
     4981 gggcgaagc
//
LOCUS       DBNGSX010000335         2584 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1690555, whole genome shotgun sequence.
ACCESSION   DBNGSX010000335 DBNGSX010000000
VERSION     DBNGSX010000335.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1690555"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="AC16CB_10110"
     CDS             complement(<1..1141)
                     /locus_tag="AC16CB_10110"
                     /inference="COORDINATES: protein motif:HMM:NF028237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HIR3342863.1"
                     /translation="MKWKDQVLLLLYIIPLLISAIIGLFLAQYLLRFRSVRGATALLA
                     MLSAASLWSLGYALEIASPDLTTNLFWAKIQYLGIVVIPPAWLIFSAQYGGSTNWLER
                     SPINQILLWIVPALTLGLVWTNELHHLVWQQAVLETVGELSILRFVRGPWFWVYVLYS
                     YSLLLLGSIWLAKIFRSRPADRRMIGLVLTGILIPWIGNLVYVTGLNPLPYLDWTPFS
                     FILASLMFSLSLFRFHLLEILPIAHKDVFDGLADLVMVLDLQNRIVDLNPAAQQLAGS
                     PASQMIGHPVDRLLGPHTDLIDQTPYFAPQQTEITMRIANVEYDFALHVSPLANERTA
                     LIGRLVVLHDITASKRAEQELRQARDGLELMVTERTAALDGVNQQL"
     gene            complement(1825..2004)
                     /locus_tag="AC16CB_10115"
     CDS             complement(1825..2004)
                     /locus_tag="AC16CB_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005986542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342864.1"
                     /translation="MNTKHTPTSEKTFACETCTMRKKAEANPKTLMARLWRWHTGWCP
                     GWKAYQAHLAEQLKP"
     gene            complement(2019..>2584)
                     /locus_tag="AC16CB_10120"
     CDS             complement(2019..>2584)
                     /locus_tag="AC16CB_10120"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3342865.1"
                     /translation="ITFGVMFAWAFIEPQFMFYVYDDLNWTSSQLGLIMSSYGVAFMI
                     GEFTLGQLSDRLGRKPVLVLGLALFSAQFVGLFTFRDATWIVVSFILAGLGNALYDPA
                     LSALILDVTPAEHKAGMMGLKGTAGSLGNMLGPGLVVLFTPFTSPQVVFLMAAVLVVM
                     LALACGLALHIPQNIEDPHYSLETADA"
BASE COUNT          653 a          653 c          716 g          562 t
ORIGIN      
        1 tgagttgctg gtttaccccg tccagcgcgg ctgtgcgctc ggtcaccatc agctccaggc
       61 cgtctctggc ctggcgaagt tcttgctcag cacgtttgct agcggtgata tcatgcagga
      121 caaccagccg gccgatgagc gccgtgcgct cattggccag gggggacacg tgaagagcaa
      181 aatcgtactc cacgttcgca attctcatcg tgatctctgt ctgttgcggg gcaaagtaag
      241 gagtttggtc gatcaggtcc gtatgaggtc ccaggagccg gtccaccggg tgaccgatca
      301 tctggctggc tggcgagcca gccagctgtt gcgcggccgg gttcaagtcg acgatgcggt
      361 tttgcaggtc gagaaccata accagatctg ccagcccatc gaagacatcc ttatgagcga
      421 tgggtaatat ctccaacagg tggaagcgga acaggctcag cgaaaacatc aggctggcta
      481 ggatgaaaga aaacggcgtc cagtccaggt acggcaatgg attcaagccg gtgacataga
      541 ccagatttcc gatccagggg atgagaatac cggtcagcac aagaccgatc attcgacggt
      601 cagcgggcct ggagcgaaaa atctttgcca gccagatcga tcccagcagc aacaggctat
      661 agctatagag cacataaacc caaaaccagg gtccgcgaac gaaccttaat atggataact
      721 caccgactgt ttccagcacc gcctgctgcc agaccaggtg gtgcagctcg ttggtccaca
      781 ccaggcccag ggtcagggct gggacgatcc acagcaggat ttggtttatg ggagaacgtt
      841 ctaaccagtt tgtggatcct ccgtactggg cggaaaaaat cagccaggca ggcgggataa
      901 cgacgatgcc gagatactgg atctttgccc agaatagatt agttgtcagg tctgggctgg
      961 cgatttcgag ggcataaccc agggaccaca ggctggcggc agacagcata gccagcaggg
     1021 ccgtagcgcc gcgcaccgaa cggaaacgga gcaggtattg agccagaaag agccctatta
     1081 tggcgctgat caataaaggg attatataaa gcagcaatag tacctggtct ttccacttca
     1141 actgcgtaac ttctattgtt aataatcgtt aacaaatgta ccataatttc cttattcgag
     1201 caattttggc ttgctgaagg cagggcaatt gcatatgccc tgaggggcat gcgcaatccc
     1261 gtgaaaagag tgtttggggt gttttggtgg ggcatacccc caccaaaaca ccccaatttc
     1321 tctccacagt ggtgggaaat cgcccaattg cgattgccct atgctgaagt tgagacaaca
     1381 gttcggacaa tatccgctag aggagtcatt gcgagccccg caggggcgcc taccctgagc
     1441 tttatatagg gtggcaacct cctggcgttt ctttgagatc gcttcgcctg ccagatggcg
     1501 gcaggcttgc gatgataggg tttcaagaaa tgtccgaact gttgtccgaa ggtaagtaca
     1561 atagcttgac aaaagcaggg aagtacatgc aatgaagggt atgcacctta aaagcgagac
     1621 cgcctagctg gaactgagcg atctcgggcg tggaaatcac gccggtcgca aatatgttca
     1681 agccaggcat gactgggata aaatcactgc atcactggaa ttattttatc attccagtat
     1741 agatcagcca ggggagtaga aataaattta ggctaatatg gagcgatgca caattgacta
     1801 cgaagtcacc aaacaacctc tttcttatgg tttcaactgc tcagccaggt gtgcctggta
     1861 agctttccag ccggggcacc agccggtgtg ccagcgccac agtcgtgcca tcagggtttt
     1921 ggggttggct tcggcctttt tcctcatggt gcaggtttcg caggcaaaag tcttttctga
     1981 tgtgggtgta tgtttggtgt tcatgttttt tctccttatc atgcatcagc tgtttctaat
     2041 gaataatggg ggtcttcaat attttgcggg atgtgcaagg ccagcccgca agcaagcgcg
     2101 agcataacta ccaagaccgc ggccatcagg aatacaacct gtggactggt aaagggtgta
     2161 aacagcacca ccaaccccgg cccaagcata tttccgaggg aaccggctgt gcctttgagt
     2221 cccatcatac ccgccttgtg ttccgctggg gtaacgtcga ggataagggc gcttaaagcc
     2281 gggtcgtaga gggcattgcc cagcccagcc aggatgaagc tcaccacaat ccaggtcgca
     2341 tcgcggaaag tgaataaccc aacgaactgc gccgagaaca gtgccagtcc cagcaccagt
     2401 acaggtttgc gccccaggcg gtcgctgagt tggccaaggg taaattcgcc gatcatgaaa
     2461 gccactccat aggagctcat gattaacccc agttgtgagg atgtccagtt cagatcatcg
     2521 taaacataga acatgaattg cggctcgata aatgcccagg cgaacatcac accaaaggtg
     2581 atga
//
LOCUS       DBNGSX010000336         1783 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_513320, whole genome shotgun sequence.
ACCESSION   DBNGSX010000336 DBNGSX010000000
VERSION     DBNGSX010000336.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_513320"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="AC16CB_10125"
     CDS             <1..728
                     /locus_tag="AC16CB_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HIR3342866.1"
                     /translation="KSLNHRPGQFLEISALGVGEAPISISSSPSRSNGFVELCVRKVG
                     DVTSALHRLRPGDKVGIRGPFGRGFPIENFRGKDILFAPGGLGLAPLRSLINQVLDER
                     AHFGRVIILYGARAPAELLFKDELRQWEARGDVELHLTVDRGDEHWKGHIGVITTLFK
                     DISVYPRNTVAVTIGPPVMYRFVLMELLGTGISEGNIWLSLERRMKCGIGKCGHCQIN
                     NVYTCQSGPSFSYKEIKGLEEAL"
     gene            725..1501
                     /locus_tag="AC16CB_10130"
     CDS             725..1501
                     /locus_tag="AC16CB_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HIR3342867.1"
                     /translation="MSQSNRPKVAFFDFTCCEGCQLTVLDSLQDHPDLLGAVEIVQFR
                     EAMSERSDDYLIAFIEGSCSRPADEERLKHIRERASIVVALGACAHLGGVNALKYFIP
                     LDDARSYVYGDKAGWYDTGDVRPVGAVIPVDVFIPGCPIDRGEFIACVKALLLGKKPP
                     VPDYPLCVECKLQENACLYTRGNICMGPVTRAGCGAICPTYGQSCESCRGFVSNPNDS
                     SMRELLAKHGLSMDEVNMQYTLYNTYSMRDELKRRQSVHG"
     gene            1494..>1783
                     /locus_tag="AC16CB_10135"
     CDS             1494..>1783
                     /locus_tag="AC16CB_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015790329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HIR3342868.1"
                     /translation="MGRRIDVHYVTRVEGHGNIVVEMGNGKLKDCRFEVVEAPRFFES
                     MLRGRHYSEAAQITSRICGICSTGHTTTSLRATEKALGVVPSEQTRLLRKLA"
BASE COUNT          383 a          431 c          534 g          435 t
ORIGIN      
        1 gcaaatccct gaaccatcgt cccggacagt tcttggagat ctctgccttg ggggttggcg
       61 aagcccctat cagcatcagc agctcaccca gtcggagcaa tggatttgtt gaattatgtg
      121 tgcgcaaggt gggagacgta actagcgcgc tgcatcgcct gagaccaggc gacaaggtgg
      181 gtattcgcgg accgtttgga cgtggcttcc caatagagaa tttccgcgga aaagatatat
      241 tgttcgcgcc tgggggcttg ggcttagcac cgcttcgctc gttgatcaac caggtgcttg
      301 atgagcgtgc tcatttcggc cgggtcatca tcctgtacgg ggcgcgcgcc ccagcggagc
      361 tgctcttcaa ggatgagcta cgccagtggg aggcgcgggg agatgtggag ttacacctga
      421 cagttgaccg tggcgacgag cattggaaag ggcatatcgg tgtgatcacc acgctgttca
      481 aagacatctc tgtctaccca cgtaacacgg tggcggtaac cattggcccc ccggtgatgt
      541 atcgctttgt gctgatggag ctgctgggta ctggcatctc agagggaaat atatggctat
      601 cccttgagag gcgaatgaag tgtggcatag ggaagtgcgg tcactgccag atcaataatg
      661 tctatacttg tcaatcaggc ccctccttta gctacaagga gatcaaaggt ctggaggagg
      721 cattatgagc cagagtaacc ggccgaaggt ggcttttttt gacttcacat gctgcgaagg
      781 ctgccagctc accgtattgg attcattgca agaccaccca gatttgctgg gtgctgtaga
      841 gatcgtccag tttcgcgagg cgatgagcga gcggagcgat gattatttga tcgccttcat
      901 tgaaggctct tgcagccgtc ctgcggatga ggaacgcctc aaacacatca gagagcgcgc
      961 ctccattgtg gtggcgctcg gcgcctgtgc ccatctgggt ggtgtaaatg ccttgaaata
     1021 ttttattccg ttagacgatg cccgctcata cgtctatggc gacaaggccg gctggtatga
     1081 cactggcgat gttcgtccgg tgggggcagt catcccagtt gatgttttta tccctggttg
     1141 tccaattgac cgaggggagt tcatcgcttg tgtaaaggct ttgctgttag gtaaaaaacc
     1201 acctgtccca gattacccgt tgtgcgttga gtgtaaactg caggagaatg cctgtctgta
     1261 tacgcgtgga aatatctgca tgggtcctgt cactcgggcg ggttgcgggg ctatctgccc
     1321 cacctatggg caaagttgcg aatcttgccg ggggtttgtc tccaatccca acgattcgtc
     1381 catgcgtgag cttcttgcca aacatggctt gagcatggac gaggtgaata tgcagtacac
     1441 gctctacaac acttattcta tgcgtgatga acttaagcgg aggcagagtg tccatgggta
     1501 ggcgtatcga tgtccattat gtaacccgcg tggaggggca cggcaacatt gtggtggaga
     1561 tgggtaatgg caagctgaag gattgccgtt ttgaagtggt tgaagcacca cgtttcttcg
     1621 aatcgatgtt gcgcggccgg cattattccg aggctgctca gatcaccagc cgtatctgcg
     1681 ggatatgttc aacagggcac accaccacct cactccgagc gacagaaaag gctctgggtg
     1741 tggtgccgag cgaacagacc cgattgctgc gaaaattggc ttt
//
LOCUS       DBNGSX010000337         2226 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_652142, whole genome shotgun sequence.
ACCESSION   DBNGSX010000337 DBNGSX010000000
VERSION     DBNGSX010000337.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_652142"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2028
                     /gene="hypF"
                     /locus_tag="AC16CB_10140"
     CDS             <1..2028
                     /gene="hypF"
                     /locus_tag="AC16CB_10140"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HIR3342869.1"
                     /translation="RYPFINCTNCGPRFTIIEDIPYDRPKTTMASFALCPDCESEYHD
                     PLDRRFHAQPVACSACGPHIWLESSSAETEPVILSSLDTAILEAQRMLAQGKIIAMKG
                     LGGFHLACDATNTEAVSRLRQRKLRIDKPFALMMPDLAAVEAHCYLNSTERELLESRA
                     RPIVILKRRPTSTIANQVAPGQDTLGVMLPYTPFHYLLFAPPPLSTTTISLTALVMTS
                     GNLVEEPIAYDNADARRRLSPLADAFLMHNRPIRTRCDDSVVRVVPYYSPSNSQNPVP
                     THKVHSVPYPLRRSRGYAPFPVHLPFEVPPILAAGSELKNTFCITRGNYAFLSHHIGD
                     MENYETMQSFEDSVAHYERLFRIRPKIIAYDLHPDYLATRYALRRAESEKLSGIALQH
                     HHAHVAACMVENGLSGDQPVIGVAFDGTGYGDDGNIWGSEFLVADYKGYRRVGHLKYV
                     PLPGGDRSIKEPWRVALAWLEQCHIGWADDMPPVKYAMTNANPAALQTLQRQLSMGIN
                     SPLTSSMGRLFDAAASLCGVHQTVNYEAQAAIEFETKADHHEQGAYSFQYLIQDDNTS
                     APLIIADPAPLFIAMVEDLRTCQPIDLISARFHNAVANLVHEVVREISRQFDLQDVAL
                     SGGVWQNMNLLYKTVALLQDDGYRVFIHNKVPTNDGGLALGQAIIAAQAFK"
     gene            2037..>2226
                     /locus_tag="AC16CB_10145"
     CDS             2037..>2226
                     /locus_tag="AC16CB_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HIR3342870.1"
                     /translation="MCLGVPGKIIEIYEVNGLQMGKIDFGGVTREACLAYVPEAQLGD
                     YTIIHVGFALNLISEQEAQ"
BASE COUNT          550 a          605 c          505 g          566 t
ORIGIN      
        1 agataccctt ttatcaactg cacaaattgt gggccgcgct ttaccatcat cgaggatata
       61 ccttacgacc gtcccaaaac cacgatggcc tcattcgccc tgtgccctga ttgcgaatca
      121 gagtatcatg atcccctcga tagacgcttc cacgcccaac cggtagcatg ttcagcatgc
      181 ggcccgcata tttggctgga aagcagctct gctgagacag agccggtcat tttatctagc
      241 cttgatacag cgatcttaga agctcaacgc atgcttgctc aagggaaaat catcgcaatg
      301 aaaggtctag gtggctttca cttagcatgc gacgccacca ataccgaggc tgtatccaga
      361 ttgcgccaac gtaaactgcg cattgataaa ccttttgcct tgatgatgcc tgatcttgca
      421 gcggtcgagg ctcactgcta cctgaattca actgaaagag agttacttga atcccgcgct
      481 cgcccaatcg taatcctaaa gcgtcgtcca acttccacta tcgccaacca agttgctccc
      541 ggacaggata cattaggggt tatgctaccc tacacgcctt tccattattt attattcgca
      601 cctccccctt tatccaccac tacaatatcc ctcacagccc tggtcatgac cagtggaaat
      661 ttggtcgagg aaccaatcgc ctacgacaac gctgatgccc gcaggcgttt atctcctctc
      721 gccgacgcct tcttaatgca caaccggccc atccgcacac gctgcgatga ctctgtagtc
      781 agggttgtcc catactattc tccctcaaat agccagaacc cagttcctac tcataaggta
      841 cattcagtac cttatcccct tcgccgctcg cgtggctatg cgccgttccc tgttcacttg
      901 cccttcgagg tgccccccat cttagctgcc ggatctgaac tgaaaaatac tttttgcatc
      961 actcgcggta actacgcttt ccttagccat cacattggcg acatggaaaa ctacgaaact
     1021 atgcaatcct ttgaagacag cgtagcccat tatgagcgcc tctttcgtat ccgcccaaag
     1081 atcattgcct atgatttgca cccggattac ctagctacgc gctacgcctt gagacgggca
     1141 gaaagcgaaa aactctcggg gatagccctg caacatcacc atgcgcatgt ggcagcttgc
     1201 atggttgaga atggtctatc cggagatcaa cctgtcattg gtgttgcatt tgacggcact
     1261 ggctatggtg atgatggaaa tatctgggga agcgagtttt tggttgcaga ttataaaggt
     1321 taccgccgtg ttggtcacct aaaatatgta cccctccctg gcggtgatcg atccataaaa
     1381 gaaccttggc gagtagccct agcctggtta gaacaatgtc atatcggctg ggcagatgac
     1441 atgcctcccg tcaaatacgc catgaccaat gcaaatccag ctgcactgca aaccttgcag
     1501 cgtcagctat caatgggtat taattctccc ttgacatcca gcatggggcg gctgtttgat
     1561 gctgctgcat cgctctgtgg tgtccaccag acggtaaatt acgaagcgca ggctgccatc
     1621 gaatttgaga cgaaagctga ccatcacgag cagggggcat actcatttca atacctgatt
     1681 caggatgaca atacatcagc tccattgatt attgcagacc cagctcctct ctttattgct
     1741 atggttgaag atctgcgcac ctgccaaccc attgatctta tatctgcccg ttttcacaat
     1801 gctgtggcca acttggtaca cgaggttgtg cgagaaatat cccgacagtt cgacttacag
     1861 gatgtggcac tgagtggtgg tgtttggcaa aatatgaacc tgctatacaa aaccgttgcc
     1921 ctgctccagg acgatggcta tcgcgtcttt attcataaca aagttcctac gaatgacggt
     1981 ggacttgcac ttggccaggc aataattgca gctcaagcgt tcaaatagga ggcagcatgt
     2041 gtttaggtgt accaggaaaa ataatagaga tttatgaagt caatggcctg cagatgggca
     2101 agattgattt tggcggcgtt acacgggaag cttgtcttgc ctatgtgcct gaagcgcagc
     2161 taggcgatta caccatcata catgtcggtt tcgccttgaa tttgatcagc gagcaggagg
     2221 cccaag
//
LOCUS       DBNGSX010000338         3462 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_444123, whole genome shotgun sequence.
ACCESSION   DBNGSX010000338 DBNGSX010000000
VERSION     DBNGSX010000338.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_444123"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="AC16CB_10150"
     CDS             <1..825
                     /locus_tag="AC16CB_10150"
                     /inference="COORDINATES: protein motif:HMM:NF023401.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 4 glycosyl hydrolase"
                     /protein_id="HIR3342871.1"
                     /translation="DIKIPRQFTSTHASPRFIPHYRAVARRAREKLRITAAGLNHFTW
                     MLDVRHPTNDQDLYPLFTRRWGELPESFEPLTRRVFADFGLFPIPGDEHLCEYLPWMS
                     DPLTRPWEKFDLSLYEWDLWANRRLEEQEQIRRLASGEMSLKRLLEQDSEGAVEIIES
                     VTGAGTQQALAVNLPNRGYIQNLPEGAIVEVPGVLSDDGVQGTEVGSLPAPIAELCRR
                     EISAAQLCVDAAVHGDRQAALQCLLLDPVINDMDLAKQVLDDYLRAYQDVLPQFWD"
     gene            842..2305
                     /locus_tag="AC16CB_10155"
     CDS             842..2305
                     /locus_tag="AC16CB_10155"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR3342872.1"
                     /translation="MPRIPLLLKYILVLCCLPIALSGCGLRELYQEKPDEGKNAASAG
                     KNAGSSARPTATLKPTATNTPVPTSTLGVEVADLQGVSIVFWHAWTGAPGDVIQSLVD
                     EFNRDNQWGISVEAVAQGSYDQVSEQVSAALENDQPPDVTTAYGYQALGWISPGLSLV
                     DLTTYVDDPVWGLDEQAQSDFYPIFWEHDLQNGERTGIPAQGSAQLLYYNNTWAKELG
                     FENPPATPEEFKAQACAANQTYKQDSDPKNDQKGGWIISTEYSAVLGWIYSFGGEIER
                     ADGNGYRFNTSPVKDAFTYMRELYEGSCSWLSESQFPEDDFATRQGLFAAGSVAGILY
                     QEEAFVEASSKDEWSVIAFPSLNGEPVISAYGPSFQVLQSTPEEQLASWLFIRWLTEA
                     GPQAKLAEVSGYYPVRASSVEMMGILPSVHPQWKSAVDLLLYARPEPALGSWRIVRWA
                     LSDAATQLFRFYFESDQLPALIKLLEETSNDLHAGLK"
     gene            2321..3094
                     /locus_tag="AC16CB_10160"
     CDS             2321..3094
                     /locus_tag="AC16CB_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR3342873.1"
                     /translation="MTYEFILTEVHDRVGLVRLNRPKALNALSSGLTRELMDALLAFE
                     ADEAIGAIVITGDERAFAAGADIKEMSEASAVEMLQRDMIGNFDAIQKITKPVIAAVS
                     GWCLGGGNELAMSCDMIVASETARFGQPEINLGVIPGAGGTQRLTRAVGKALAMEMVL
                     NNRTLTASEALHYGLVNRVVPVERYLQEALELATEIAARAPLAVRMGKEAVNHAFETT
                     LAEGMADERRAFYFLFASQDQKEGMAAFTEKRKPQWKGM"
BASE COUNT          755 a         1023 c         1024 g          660 t
ORIGIN      
        1 gatatcaaaa tccccaggca gttcaccagc acgcacgctt cgccgcgctt catcccgcat
       61 taccgggcgg tcgctcgccg ggcacgcgag aagctgcgca tcaccgcggc cgggttgaac
      121 cacttcacct ggatgctcga cgtacgccac cccacaaatg accaagacct gtacccgctt
      181 ttcactcgcc gctggggcga gctgcccgag agcttcgagc cgctcacccg ccgcgtattc
      241 gccgatttcg gtctattccc catccctgga gacgagcacc tatgcgagta cctgccctgg
      301 atgagcgacc cactcacacg cccctgggag aagttcgatc tcagcctgta tgagtgggac
      361 ctgtgggcaa accgccgcct ggaagaacag gagcagattc gccgccttgc ctctggcgag
      421 atgagcctga aaaggctgct ggagcaggac agcgaaggcg cggtggagat catcgagagc
      481 gtgaccgggg cgggcacgca gcaggcattg gcggtcaacc tgcccaaccg cggttatatc
      541 caaaacttgc ccgaaggcgc catcgtcgaa gtgccagggg ttttgagcga cgacggagtg
      601 cagggcactg aggttggctc gctgcccgcg ccaattgccg aattgtgccg gcgcgagatc
      661 agcgccgccc agctttgtgt agacgctgcc gtgcatggcg accgtcaggc agcgctgcaa
      721 tgcctgctcc tggacccggt gatcaatgac atggacctcg ccaagcaggt gctggacgac
      781 tatttgagag cgtatcaaga cgtgctcccc cagttttggg attgaagaag gcaagcagga
      841 catgccccgc atccccctgt tgctgaaata tatactcgtg ctgtgctgcc tacccatagc
      901 cctgagcggc tgcgggctca gagagctata tcaagaaaaa ccggatgaag gtaaaaatgc
      961 ggcctccgcc ggcaaaaacg ctggctccag cgccaggcca accgccaccc tcaaacccac
     1021 agcaaccaac acgcccgtgc cgaccagcac cttgggcgtc gaagtcgcgg acttgcaggg
     1081 cgtgtccatt gtattctggc atgcctggac gggcgccccg ggagatgtga tccaaagcct
     1141 ggtggatgaa ttcaaccgcg acaaccaatg ggggatcagc gtggaggctg tcgcgcaagg
     1201 cagttacgat caggtttccg agcaagttag cgcggctcta gagaacgacc agccgccgga
     1261 tgtcaccacc gcctacggct accaggcgct tggctggatt tcacccggtt tatcgctggt
     1321 agatttaacc acttatgtcg acgacccggt gtgggggctg gatgaacagg cgcaatccga
     1381 cttttacccc atcttctggg agcatgacct gcagaatggg gagcgcaccg gcatccccgc
     1441 ccagggctca gcacagctgc tgtactacaa caacacctgg gccaaggagc tggggtttga
     1501 aaatccgcct gcaacgccag aagagttcaa ggcgcaggcc tgcgccgcaa accaaaccta
     1561 caagcaagac agcgacccca agaacgacca gaaagggggc tggatcatct ccacggagta
     1621 ctctgcagtt ttgggctgga tctattcctt cggcggcgag atagagagag cagatggaaa
     1681 tggctaccgc ttcaacacct cgccggtaaa ggatgccttc acctacatgc gagagctcta
     1741 tgagggcagc tgctcctggt tgagcgagag ccaattccca gaggatgatt ttgccacccg
     1801 ccagggcttg tttgctgcgg gctcggtggc gggcatcctt taccaggaag aggcattcgt
     1861 cgaagcgagc agcaaggatg agtggagtgt gatcgctttt ccatccctaa atggcgagcc
     1921 ggtgatctcg gcctacgggc catcctttca ggtgctccaa tccacgcccg aagagcaact
     1981 ggcgagctgg ttgtttatcc gctggttgac cgaagcgggg ccccaggcta aactggcaga
     2041 ggtgagtgga tactacccgg tgcgcgcctc cagcgtggag atgatgggca tcttgccctc
     2101 cgtccacccg caatggaaga gcgcagtgga cctgctcctc tacgcgcgcc ccgaacctgc
     2161 gctggggtcc tggcgcatcg tccgttgggc gctgagcgac gcagccaccc agctatttcg
     2221 cttctacttt gagagtgatc agctgcctgc tctgataaaa cttctcgaag agacttcaaa
     2281 cgacttgcat gctggattga aatagattaa ggaggggttt atgacctacg aatttattct
     2341 caccgaagtc cacgaccgtg tggggctggt gcgattgaac cgccccaaag ccctcaatgc
     2401 cctgagtagc ggcctgaccc gcgagctgat ggatgcccta ttggccttcg aggccgatga
     2461 ggcgatcggc gctatcgtta ttacgggtga cgagcgcgcc tttgctgcag gagcagacat
     2521 taaggaaatg tccgaggcct cagcggtcga gatgctgcaa cgcgatatga tcggcaactt
     2581 cgatgccatc cagaagatca ccaaaccggt catcgctgcc gtatcgggct ggtgtctggg
     2641 cgggggcaat gagctggcta tgtcttgcga catgatcgtg gcctccgaaa cggcgcgctt
     2701 cgggcagccc gagatcaacc tgggtgtgat cccgggggca ggcggcacac aacgcctgac
     2761 ccgggctgtg ggcaaagcgc tcgccatgga gatggtgctg aacaaccgca ccctaaccgc
     2821 ctcggaggct ttgcactatg ggctggtcaa ccgcgtggtg ccggtggagc gctatctgca
     2881 agaggccctt gaattggcaa ccgagatcgc cgcccgcgcc ccacttgcag tgcgcatggg
     2941 caaggaggcg gtcaaccatg ccttcgaaac caccttggct gagggtatgg ctgacgagcg
     3001 ccgcgcattt tacttccttt tcgccagtca agatcagaaa gaggggatgg ccgcgttcac
     3061 agaaaagcgc aaaccacagt ggaaggggat gtagccctca caggctctgt tcgccaggct
     3121 ctgatgcaga cacagggatg atccggcgcg ccagcgcagc ctagcctgag attttggcgc
     3181 ccctgtgcag cctagctggt caccgggctg cagcgtcatc cacctgttga cttttttgat
     3241 aacctaagta caataatttc agggaatatt gccaaagtta cgcagttgga gaggtccttc
     3301 tctcttataa cagcgtaagc ctaaacggtt caatataagc agaggagggt gccatgcaaa
     3361 ccaaaaaaat cgtcttaacc acaattgcca tccttttggc gttatccgtc atcgcggcca
     3421 gcgttttgcc cgctacgacc agcaccatca agaaaggcga ga
//
LOCUS       DBNGSX010000339         3120 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_29040, whole genome shotgun sequence.
ACCESSION   DBNGSX010000339 DBNGSX010000000
VERSION     DBNGSX010000339.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_29040"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1300)
                     /locus_tag="AC16CB_10165"
     CDS             complement(<1..1300)
                     /locus_tag="AC16CB_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3342874.1"
                     /translation="MKQSKVVLTIFSLMVIVSMVTACGKATPMPAPPTAPPEAPAATE
                     APPEAPAATEAPAEAPAATEAPAEVGKPPAITVADMQGVAPGKYPNQYELAEFEAATG
                     GTMEFSGRSTIDPVLVEIYGDIPADVTARLPEEPLVVVPYAEIGKYGGQLEGLSLGPE
                     SGNSEYLSWRHANFVRYAEDLVTIAPNVVKDYQVSDDLTEFTFTLRKGHKWSDGEPFT
                     TADVQFWWEDIILNKDLTPEVPTVWTFGGEPMKVEIVDDVTFKFVTAAPAPGMMPWLA
                     RTWILPAAPRHWLESKHLKYNPKINDEAVAAGKESWVDYFYTFHGEWQDSVHRWTEGV
                     PRLEAWVVVEETPEYQLAMANPYYFKVDTAGQQLPYADNSRESYSQDAQVLELKVING
                     EVDQKAQTLSFRSAPVFKEHEADGNYVVTLSPSGSDGMNVA"
     gene            complement(1423..2208)
                     /locus_tag="AC16CB_10170"
     CDS             complement(1423..2208)
                     /locus_tag="AC16CB_10170"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR3342875.1"
                     /translation="MSLAVFVMVDGLRPEPLLEDRYTNLYSLRQRSSWTLNASSLMPT
                     ITLPCHTTIFHSVPPSRHGITTNKWVPMKAPIPGLVEVAKAAGLRSGFFYNWEKLRDV
                     SRPGSLDFTYFKDNVYTDPNGEEILVDEALKYQANDHPDFIFFYFGTVDVAGEAYGWM
                     TDKYFDQIEHVDGLLGRLLNGLPPDATVLLHADHGGHDKSHGTDAPEDTTIPWMVAGP
                     GIRRDYEIQSKVTLLETAPTLVRILGIDPHPDWEGHCVDEIFE"
     gene            2808..>3120
                     /locus_tag="AC16CB_10175"
     CDS             2808..>3120
                     /locus_tag="AC16CB_10175"
                     /inference="COORDINATES: protein motif:HMM:NF018012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR3342876.1"
                     /translation="MNNQPIQCDFFRILTQEGMEAIHDASIAVLEKTGVTVQHSEGLK
                     MLEAAGAAIDWDTRRARIPESLVEKCLSSLPSQITFGARNPKKDLLAGHGLLPITRNG
                     GG"
BASE COUNT          728 a          800 c          803 g          789 t
ORIGIN      
        1 aggccacgtt catgccatcc gagcccgacg gggataaggt cactacgtaa ttcccatcgg
       61 cctcgtgctc tttgaagaca ggggctgatc taaagcttag ggtctgggct ttttggtcca
      121 cctcgccgtt gatgactttc agctcgagca cctgggcatc ctggctgtag gattcccggc
      181 tgttgtcagc gtagggcaac tgctgcccag cggtgtcaac cttgaagtaa taggggttgg
      241 ccatcgccag ctggtattct ggggtttcct caacgactac ccaggcttcc agacgtggta
      301 caccctccgt ccaacgatgc acgctgtcct gccactcgcc gtggaaggtg tagaagtaat
      361 ccacccagga ttccttgcct gctgcgacag cttcgtcgtt gatcttcggg ttatacttga
      421 ggtgcttgga ttctagccag tggcgggggg cagccggtaa gatccaggtg cgcgccagcc
      481 agggcatcat gccgggagcc ggagctgcgg tgacgaactt gaaggtgacg tcatccacaa
      541 tttcaacctt cattggctcg ccgccaaagg tccatacggt gggcacttcg ggggtcaggt
      601 ctttgttcag gatgatatct tcccaccaga actggacgtc ggctgtagtg aagggttccc
      661 catcggacca cttgtgcccc ttgcgcagtg tgaaggtgaa ctccgtcaga tcgtcactga
      721 cctgatagtc cttgaccacg ttgggggcga tcgtcaccag gtcctcggca tagcggacaa
      781 agttggcatg ccgccagctg aggtattccg agttgccgct ctcgggtcca agcgacaatc
      841 cttctagctg accgccgtat ttgccgatct cggcgtaggg cacgactacc aagggttctt
      901 cgggcagacg cgccgtcaca tccgccggga tgtcgccgta tatctccacc agaactggat
      961 caatcgtgct tcggccgctg aattccattg tgccgccggt ggcggcttca aattctgcaa
     1021 gctcatactg gttggggtat tttcccgggg caacaccttg catgtcggca accgttatag
     1081 ccggcggttt gccaacttcg gcaggtgctt cggtggcggc aggtgcttca gcgggcgcct
     1141 cggtggcagc aggtgcttca gggggtgcct cggtagcagc aggtgcttct ggaggggcag
     1201 tcggtggtgc gggcatcggc gttgccttcc cacatgctgt caccatcgag acaatcacca
     1261 taagggaaaa gattgtcaga acaactttac tttgtttcat ttttccactc cttatctaat
     1321 tagaagctaa tggtacaaat ttgggcgatc tagtatttct cctctatgac ctcctttctt
     1381 tggcctatcc tggcacagga aattaccagg ttcgggtgac aactattcaa aaatctcgtc
     1441 aacacaatgc ccttcccagt caggatgagg atcaatcccc aaaatacgaa caagcgttgg
     1501 ggctgtttcc aacaaggtaa ccttggattg gatttcgtaa tcacggcgaa tccctggtcc
     1561 agccaccatc catgggatcg tggtgtcttc gggggcatct gtgccgtgac ttttatcatg
     1621 cccaccatgg tcggcgtgca ggagaactgt ggcatcaggc ggcaatccat tcagcagccg
     1681 tcccagtagg ccatccacat gctcgatttg gtcaaagtat ttatcagtca tccagccata
     1741 agcttcgcca gcgacgtcga cagttccaaa gtaaaaaaat ataaaatctg ggtggtcatt
     1801 cgcttggtat ttaagcgcct cgtcgacaag gatttcctca ccatttggat ccgtgtaaac
     1861 gttgtccttg aaatacgtaa agtccaggct gccgggtctg gagacatccc taagtttctc
     1921 ccagttgtaa aaaaatccac tgcgtaatcc ggctgcttta gccacttcga ccaagccggg
     1981 gataggggct ttcatcggca cccatttatt cgttgtaata ccgtgccttg atgggggaac
     2041 actatgaaag atggtcgtgt ggcagggtaa tgtaatcgta ggcattaaac ttgacgcgtt
     2101 taatgtccaa gatgatcttt gacgcaagga gtatagattt gtatatcgat cttcaagaag
     2161 tggctcagga cgcagaccat ccaccattac gaatacagca agcgacatga ttcagttcct
     2221 catctcgagt attagcaaac tcttcattga taatgatact gagaacaaga attagctctt
     2281 gttttctatg gttatgtact ttcaatattc gcagtgtaga taaactcccc tcctgtgtcc
     2341 ttgtttccct aatatagctc gggcgctaaa catgggacaa tgtatattgt ccatgtgaac
     2401 taatccccct tttgaagtcc tgtacttcgg aacatccgcc caggaaagcc gattgttaag
     2461 atatgcacgt agaaattcaa tgtctcgttg agaagaactg cagccccgaa aggattggat
     2521 gaaacccttt gaaagcgtaa gctgaatcag gatagcacat ataccccttg cctgtcaata
     2581 atttatggac aaaagaacac atactttaat gaaagctttc gatagcaccc caaaaaccct
     2641 gccctatccc aaggtgtcaa cagtggattt tctgctgctc atccactgtg gtttgtttca
     2701 ttgtacaatt gagagcaagg gccacaagtg ggataatctc tcggatttcg aaagactgct
     2761 tggtttttgc ggattgtcaa gccaataact atggcacaag gagcaccatg aacaaccagc
     2821 ctattcaatg cgatttcttt aggattctta ctcaagaggg gatggaagcc attcacgatg
     2881 catccatagc agtgttagaa aagaccggcg tcaccgtcca acattccgaa ggtctgaaga
     2941 tgttagaggc ggcaggagca gcgatcgatt gggatacccg gcgggcgaga atacccgaaa
     3001 gcctggtgga aaagtgtctt tcctccttgc catcccagat tacctttggc gcgcgaaatc
     3061 cgaagaaaga cttgctggct gggcatggtc tcttacccat cacacgcaat ggcggcggta
//
LOCUS       DBNGSX010000340         3265 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1691572, whole genome shotgun sequence.
ACCESSION   DBNGSX010000340 DBNGSX010000000
VERSION     DBNGSX010000340.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1691572"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="AC16CB_10180"
     CDS             <1..350
                     /locus_tag="AC16CB_10180"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HIR3342877.1"
                     /translation="AIEHAGGVAHEIAVYNTLPAQPDPQELRDLQLGVDIVTFTSSST
                     VRNFVSLARQAGLDHQSLPGEPLIACIGPITAQTALEQGLNVDLVASEYTTQGLIQAL
                     LEFPSLQITPGAS"
     gene            389..1453
                     /gene="hemB"
                     /locus_tag="AC16CB_10185"
     CDS             389..1453
                     /gene="hemB"
                     /locus_tag="AC16CB_10185"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HIR3342878.1"
                     /translation="MIVDTPSPTQTYTRPTARPRRLRLTSALRGMVRETELNPRDFIY
                     PLFIRPGWGIQNEIKSMPGVYQWSVDRLPAEIESIAKLGIPAVILFGIPSMKDPVGEE
                     NFAPNGIVQEAIRAIKQTVPEMVVITDLCLCEYTDHGHCGVLNVGDELPHPHLPEGYV
                     LNDPTLDVLAQAAVSHAEAGADIVAPSGMMDGMVAAIRETLDAARFEHIPILSYAVKY
                     ASAFYGPFRDAAESPPKFGDRKSHQMDPANAREALRETALDVAEGADMLMVKPALPYL
                     DIIHRVREAFPELPLAAYNVSGEYSMIKAAAANGWLNEQLAVMEGLTAIKRAGADLIL
                     TYHAKDAARWLQNMNGSHHG"
     gene            1521..2600
                     /locus_tag="AC16CB_10190"
     CDS             1521..2600
                     /locus_tag="AC16CB_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04053 family radical SAM/SPASM
                     domain-containing protein"
                     /protein_id="HIR3342879.1"
                     /translation="MVHADFDLAPFTIAWEVTRACAFACKHCRADAQHRPDPKELTTD
                     EALHLIDQLADFGSPILIFTGGDPMMRRDLFDLIAHATQRGLRCSLTPTATALPTIDR
                     LTKAKEAGIRRIALSLDAPNPEVHDEFRQVKGSWLRTMKILKNAQSIGLSVQVNTTVS
                     RFNANLLPDMVPFIQEVGAVQWSVFFLVPTGRAQSEWMISPDEHERVFNWLFDLSQNA
                     PFDIKATAAPMYRRVAIQRKKAETIGDAQVTFQGAGFQYADGLNRPTKGVNDGNGFLF
                     ISHLGEIMPSGFLPISAGNVRSDNVVDVYRHHPLFKALRDPAQIKGKCGICDFRDVCG
                     GQRGRAYGLTGDYLETDPACAYEPN"
     gene            2587..>3265
                     /locus_tag="AC16CB_10195"
     CDS             2587..>3265
                     /locus_tag="AC16CB_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR3342880.1"
                     /translation="MNQTEPQSRFLRACQRLPVDTTPVWFMRQAGRYMPEYRAIREKY
                     SLLEIVQHPEIAVEVTLQPVHALGVDAAILFADILLPIVPMGLHLEFAKGEGPVISNP
                     VRTAADITRLRPIDADTDLGHVMEAIRILRGELPASVPLIGFAGAPFTLASYMIEGGS
                     SRDYRLAKTLMQSEPGLWAEFMGKLATSLENYLLAQVRAGAQAVQLFDSWVGALSPQD
                     YADFVLPY"
BASE COUNT          710 a         1004 c          856 g          695 t
ORIGIN      
        1 aagccattga gcacgccggg ggggtggcac acgagatcgc tgtctacaac accctgccag
       61 cccagcccga cccgcaggag ctgcgcgact tgcagctggg tgtggacatt gtgaccttca
      121 ccagctcctc caccgtgcgg aattttgtca gcctggctcg ccaggccggg cttgatcatc
      181 agtcactacc cggcgagcca ctcatcgcat gcatcgggcc gatcacagcc cagaccgccc
      241 tagagcaagg attaaatgta gatctggtcg ccagtgagta cacaacccag ggtttgatcc
      301 aggcattatt agaattcccc tctctacaaa taacaccagg tgcatcatga tcaattggaa
      361 aacatctcaa caaaaacagg taaaaccgat gattgtagac actccatcac ctacccaaac
      421 ctacacccgc cccaccgccc ggccacgccg actgcgcctc acttctgcct tgcgaggcat
      481 ggtgcgcgag actgagctaa acccgagaga ttttatctac ccgttgttca ttcgacccgg
      541 gtggggaatc caaaacgaga tcaaatccat gcccggcgtg taccagtggt cagtcgaccg
      601 tttgcctgca gaaatagagt caatcgctaa actgggcata ccagctgtca tcttgtttgg
      661 catcccgtcc atgaaggacc cggttggcga ggagaacttt gccccaaatg ggatcgtcca
      721 ggaagccatc cgcgctatca agcaaacggt acctgagatg gtggtaatca ccgatctttg
      781 cctatgcgaa tataccgacc atggacattg cggtgtgctc aacgtgggcg atgagctgcc
      841 ccacccccac ctgccagagg gctacgtgtt gaacgaccca accctggatg tcttagccca
      901 agccgccgtc tcgcacgccg aggctggcgc ggatatcgtc gccccttccg gaatgatgga
      961 cggcatggtg gcagctatcc gagaaaccct ggatgcagct cgcttcgagc atatccccat
     1021 cctatcttat gctgtcaagt atgcctcagc tttctatggt cctttccgtg atgcggctga
     1081 atcgccacca aaattcggtg accgcaaatc tcaccagatg gatcctgcaa atgcgcgcga
     1141 agccctgcgc gaaactgcgt tggatgtcgc tgaaggagcc gacatgctga tggtcaagcc
     1201 agccttacct tacctggata tcatccaccg cgtgcgggag gctttcccag agttgccttt
     1261 ggcagcctac aatgtcagcg gcgagtacag catgatcaag gcagccgcag ccaacggctg
     1321 gctgaacgag cagttggctg tcatggaagg ccttacagcc atcaagcgcg ccggtgccga
     1381 cctgatcctt acttaccacg ccaaagatgc ggctcgctgg ctacaaaaca tgaatggatc
     1441 gcaccatgga taaattttcc gctccccacc cttcactagc ccctgatgca gccgctgagc
     1501 gacaatacca cgatcagcgc atggtacacg ccgatttcga tctggctccc ttcaccatcg
     1561 cctgggaagt gacccgcgct tgcgcttttg cctgcaagca ctgccgcgcc gacgcccaac
     1621 atcgccctga cccaaaagag ctaaccaccg acgaggcgct acacttgatt gatcagctgg
     1681 cggattttgg tagcccgatc ttgatcttca ctggtggcga tcccatgatg cgccgcgacc
     1741 tgtttgattt gatcgctcat gccacccaga gaggcctgcg ttgctcgctg acccccactg
     1801 ctaccgcctt gccaaccata gatcgcctta ccaaggctaa ggaggccggc atacggcgca
     1861 tcgccctgag cctggacgct cctaacccag aagtgcacga tgagttccgc caggtgaaag
     1921 gctcctggct gcgcaccatg aaaatactga agaacgcgca atccatcggg ctgagtgtgc
     1981 aggtaaacac aaccgtgtca cgcttcaatg caaacctgtt acccgacatg gttccattta
     2041 ttcaagaggt gggcgcagtg cagtggtcag tgttcttcct cgtacccaca gggcgcgcgc
     2101 aatcagaatg gatgatctcc ccggatgagc acgagcgtgt cttcaactgg ctctttgact
     2161 tatcccaaaa cgctccattc gatatcaagg ccacagcagc gcccatgtat cgccgtgtcg
     2221 ccatccaacg caagaaagcc gagactatcg gagatgctca ggtgacattt cagggtgctg
     2281 gcttccaata cgctgatggt ttgaaccggc ccaccaaggg cgtcaacgac ggcaacggtt
     2341 tcctgttcat ctctcacctg ggtgagatta tgccgtctgg cttcctgcct atctcggcgg
     2401 gcaatgtacg cagcgataat gtggtcgatg tataccgcca ccatccgctg tttaaagccc
     2461 tgcgcgatcc ggcgcaaatc aagggtaagt gcgggatttg tgacttccgg gacgtctgcg
     2521 gtggtcaacg tgggcgtgcc tacggtttga ccggagatta tctagaaacg gaccctgctt
     2581 gtgcctatga accaaactga accccaatcc cgctttctac gcgcctgcca gcgcctgcca
     2641 gtagatacca cccccgtgtg gtttatgcgc caggcaggtc gttatatgcc agaataccgc
     2701 gccatccgtg agaaatactc actcctggag attgtgcaac atccggagat cgccgttgag
     2761 gtgacgctgc agccggtgca tgctttgggc gtagatgctg ccatcctatt cgcagacatc
     2821 ctgctgccga tcgtccccat gggcttacac ctggaatttg ctaagggtga gggaccagtg
     2881 atctccaatc cagtgcgaac agctgccgat atcacccgcc tgcgcccgat cgatgccgat
     2941 actgacctgg gtcatgtgat ggaagctatc cgcatcctgc gcggcgagct acccgccagc
     3001 gtacccctga tcggtttcgc cggcgctcca ttcacactgg cttcctatat gatcgagggc
     3061 ggttcttcgc gcgattaccg tctggccaag accctgatgc aatccgagcc ggggttgtgg
     3121 gcagaattta tgggcaaact ggcgacctct cttgagaatt accttctcgc acaggtgcgc
     3181 gctggagccc aggcggtaca gcttttcgac tcgtgggttg gcgcgctcag cccgcaggac
     3241 tatgcggatt tcgtcctgcc ttatt
//
LOCUS       DBNGSX010000341         4650 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_167534, whole genome shotgun sequence.
ACCESSION   DBNGSX010000341 DBNGSX010000000
VERSION     DBNGSX010000341.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_167534"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="AC16CB_10200"
     CDS             complement(<1..855)
                     /locus_tag="AC16CB_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3342881.1"
                     /translation="MSIKNIAILGAGIGGMAAAYDLRRAGHQVTIYEAADFVGGLASG
                     FKEPHWDWYVEKYYHHWFASDRHILGLINELGWRDQVLFPRPYTVVYHKWQFYPFDSI
                     LTALMFPGLGWGIHKLRFGLVGLYLKLTNRWQPLESVTVDDWMRRWAGDRVYELMWEP
                     LIIGKFGERYAKQVNMAWLWARLKARTTRLGTFKGGFQTFANRFANSLREMGVHIHLS
                     TPINHIEPQAAGLLTLTTPTNPVQFDQCLVTTSPALLAHLAPSLPPEYLSGLLNLKSM
                     GAVVMVLSL"
     gene            complement(852..1880)
                     /locus_tag="AC16CB_10205"
     CDS             complement(852..1880)
                     /locus_tag="AC16CB_10205"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR3342882.1"
                     /translation="MRRWQFWLGVVISLLFLYVALRGLHLEDLEASFTEANYWWLIPG
                     VGVYFLGVWARAWRWHYLLRPVKPIPTRVMFPIVAIGYMGNNIYPARAGEVLRAVVLK
                     RRQGVPVSASLATIIVERVYDGVVMLAFVFLNLPELARLTADSGFVGNIQTLALWGAG
                     AFVGALGVFLLAAMFPEQAEAILTTLVQRLVPARIRDRTLSLGQRFLGGLQALRSPRE
                     AMMVFFTSVVIWLLETGKYWFVMHAFPFEVSFFALMLMNGIVNLATTIPSAPGYVGTF
                     DAPGIAVLAAYGVERAIAAAYTLVLHVALWVPITALGAYYFAREGLHWGEGVGKIEKD
                     EVERVKVS"
     gene            1981..2490
                     /locus_tag="AC16CB_10210"
     CDS             1981..2490
                     /locus_tag="AC16CB_10210"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR3342883.1"
                     /translation="MLTLNQLAPDFLLPDLDGTLHSLRNYREQVVVLNFWSAECPHAA
                     RTDAQLVANQKTWGSEVVLISIASNANEPPELLRQVANQEGLTLILLDQGSDVADRYG
                     AITTPHLFVIDEKGILRYQGAFDDINFRKRSATKHYLYDAVEAILAGALPNPAETPPY
                     GCAILRHVI"
     gene            complement(2543..3478)
                     /locus_tag="AC16CB_10215"
     CDS             complement(2543..3478)
                     /locus_tag="AC16CB_10215"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HIR3342884.1"
                     /translation="MRIAVFGTGGVGGYFGGRLARAGEDVHFIARGEHLRAIQTGGLR
                     VDSILGDFIVKPAQASDEPSQVGVVDVVILGVKAWDVPNAAQAMRPMVGDQTFVVPLE
                     NGVDAPEQLASVLGREHVMGGLCQISAFIAGPGHIRHVGIEPFVAFGELDGRPSQRAE
                     GLLQVLEAAGVKAAIPPDIQVSMWEKFLFIATVSGLGAVTRAPADIYRSLPETRQMLI
                     AAFEEIVALARARGVMLPEDTVEKRLAFVDNLPDGLLASMQRDILEGKPSELASQNGA
                     VVRMGAELGVPTPVHSFIYNCLLPQELRARGQLKF"
     gene            3655..4401
                     /locus_tag="AC16CB_10220"
     CDS             3655..4401
                     /locus_tag="AC16CB_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR3342885.1"
                     /translation="MSDKETAQEVIESYRKRQQQARKAPLIIGIASVLLVIGAAVIIF
                     WMLGPDAPAISLFATETPTPTATSTSTSTPTITSTATQTATASNTPTETVTPTPEGPF
                     VYEVVQGDTLSSIAEKFGVDLFALIAVNNLDPTLPIDIGDQLTIPGSDTELPTETPIP
                     LSMRRGTIIEYIVKPGENLASIAEKFNSTVDDIVSENEIENPNEIQAGTKLNVPVNLV
                     TPLPTNTAGPSPTATTAVTPADTPTLTATP"
BASE COUNT         1106 a         1367 c         1201 g          976 t
ORIGIN      
        1 aagggagagc accatgacca ccgcgcccat gctttttaga ttgagcaggc cgcttaaata
       61 ctctggcggt agagatggag ccaggtgtgc cagcaacgcc ggcgaagtgg taactagaca
      121 ttgatcgaat tggacagggt tggtaggggt tgtcagagtg agtaagccag cggcctgtgg
      181 ctcgatgtgg ttgataggtg tagacagatg gatgtgcaca cccatctcac gcaaagagtt
      241 ggcgaaacgg ttggcaaaag tctggaagcc tcctttgaag gtgcccagtc gggtggtgcg
      301 agctttcaag cgcgcccaca gccaggccat gttgacctgc ttggcgtacc gctcgccaaa
      361 cttcccgatg atcaatggct cccacatcag ctcgtaaacg cggtcaccag cccagcggcg
      421 catccagtcg tctacggtga cactttccag aggttgccag cggttggtaa gcttgaggta
      481 gagcccaacc aagccgaagc gcagtttatg gataccccag ccaagccccg ggaacatcaa
      541 ggcggtcagg atcgaatcga agggataaaa ttgccacttg tggtacacca cagtgtaagg
      601 gcgcggaaag agcacctggt cgcgccagcc cagctcattg atcaagccca agatatgtcg
      661 gtcgctggcg aaccagtgat ggtagtactt ctcgacgtac caatcccaat gcggctcttt
      721 gaaaccagat gccagcccgc cgacaaaatc tgctgcctcg tatatggtca cctggtgacc
      781 ggcgcgccgc aggtcatagg cggctgccat gccgcctatg ccagcgccca agatggcgat
      841 attttttata ctcaactgac ctttaccctt tccacttcgt ctttttcaat tttacccacc
      901 ccctctcccc agtgcaaacc ctctctggcg aagtaatatg cccccagggc agtgatcggg
      961 acccacaggg cgacgtgcaa gaccagcgta taggctgcgg ctatggctcg ctccactcca
     1021 taggcggcta gaacggcgat accaggcgca tcgaacgtgc ccacgtaacc cggcgctgaa
     1081 gggatggtgg tagctaagtt gacgatgccg ttcatcagca tcagggcgaa aaagcttacc
     1141 tcgaatggga aagcgtgcat cacgaaccag tacttgcctg tttctagcag ccagatgaca
     1201 acagacgtaa agaaaaccat catcgcttcg cgcggtgagc gcagtgcctg taatcctccc
     1261 aggaagcgct gtcccaggct gagggttcga tcgcggatgc gcgctgggac caagcgttgc
     1321 acaagggtcg tcagaattgc ttcggcttgt tccgggaaca tggcagccag aagaaagacc
     1381 cccagcgcac ccacaaatgc gcctgcaccc cacagtgcca aagtctggat attccctaca
     1441 aagcccgaat cagcggtcag ccgggccagc tcgggcaagt tgaggaacac aaaggccagc
     1501 atgaccacac catcgtatac gcgttccaca atgatagttg ccagagaggc tgatacggga
     1561 acgccttggc ggcgtttaag caccacagcg cgcagcactt cgcctgctcg ggcaggatag
     1621 atgttattgc ccatatatcc gatggcgaca atagggaaca tcacacgtgt gggtattggt
     1681 ttgaccggtc ggagcaagta atgccagcgc caggcgcgtg cccacacccc aaggaagtac
     1741 acgcccaccc caggtatcag ccaccaataa ttcgcttctg tgaaggaggc ctccaaatct
     1801 tccagatgca ggccgcgcag cgcgacgtat aaaaaaagca ggctgatgac cacgcccaac
     1861 cagaattgcc agcgtctcat ggcgctcatt ctaccaccga tgccttatca cttcgtcttt
     1921 cattctagtt gggctggttg cagctcaata cccgcgcaga gattgattta taatctcacc
     1981 atgcttacac tcaatcaatt agcccctgac ttcttattac ctgatcttga cggtaccttg
     2041 cattcgctgc ggaattaccg ggaacaggtt gtggtgctca atttctggtc ggcagaatgc
     2101 cctcatgcag cccgtaccga tgcccagttg gtcgcgaatc agaaaacatg gggatctgag
     2161 gtagtgctca tctcgatcgc cagcaatgcc aacgagcccc ctgaactcct caggcaggtt
     2221 gccaaccagg aaggccttac tttgatctta ctggatcagg gaagtgatgt ggcggatcgt
     2281 tatggggcaa tcaccacgcc ccatctattt gtgatcgatg aaaaagggat tttgcgctac
     2341 cagggcgctt ttgacgacat caactttcgc aaacggtcag caacgaagca ctatctgtac
     2401 gacgcggtgg aagccatcct ggctggcgcg ttgcccaacc cggcggagac acccccctat
     2461 ggctgtgcta tcttgcgaca tgttatttga tcattggggg tataggcagt taataaggaa
     2521 caacccccaa ttgagtaatt ttttagaatt tcagttgacc gcgggcgcgc agttcctgag
     2581 gcagcaagca gttatagatg aaggagtgca ctggcgtggg gacgccaagc tctgccccca
     2641 tgcgcaccac ggcgccattt tgtgaagcca actcagaagg ttttccctcc aggatatccc
     2701 gttgcatcga tgccagcaag ccatccggta agttgtcgac gaaagccagg cgtttctcga
     2761 ctgtatcttc ggggagcata accccacgtg cccttgccag ggcaacgatt tcttcaaatg
     2821 ctgcgatcag catctggcgt gtttcaggca ggctgcgata aatgtctgcc ggagcccgtg
     2881 ttaccgcgcc caatccactc accgtggcga tgaacaagaa tttttcccac atcgatacct
     2941 ggatatcggg cgggatagcg gcttttacac cggcggcttc caaaacttgt aacaggccct
     3001 cagcgcgctg gctggggcgc ccgtctagct caccgaaggc cacaaaaggc tcgatgccga
     3061 catggcggat atgccccggc ccggcgataa aagccgagat ctggcacaga ccccccatga
     3121 catgctctct ccctaagacc gaggcgagct gctcgggtgc atctacgcca ttctccagcg
     3181 gcacgacgaa ggtttggtct cccaccattg ggcgcattgc ttgagctgca ttgggcacgt
     3241 cccaggcctt cacccccaag attacaacgt ccaccacgcc gacctggctt ggctcgtcgc
     3301 ttgcctgggc aggttttacg atgaaatcgc cgagtatgct atccacgcgc aagccaccgg
     3361 tttggatggc gcgcaggtgc tcgccgcggg cgatgaaatg cacgtcttcg ccagccctcg
     3421 ccagccgccc gccaaagtat cccccgacgc cgccggtgcc aaataccgca attctcatcc
     3481 ttacctcctg aggctttttt atatattcct attcccatcc cggtatctat tctcaccccg
     3541 agggggctga gggagtgccc attgattata taccgattcg ccaaatgcac gattatagag
     3601 taaaatcaca aaaaattcga tctttcctgc tagcttagct cgaagggaaa aacaatgagc
     3661 gataaagaaa ctgcccaaga ggtaattgaa agctatcgca agcgccaaca acaagcgcgc
     3721 aaagcacctt tgatcatcgg gattgcttcc gtgctgcttg tgattggggc tgctgtgatc
     3781 attttctgga tgcttgggcc agatgcgccg gccatctcac tgttcgccac tgagactcca
     3841 acaccaacgg caacatcaac ctccacgtca accccaacca tcacctccac cgccactcag
     3901 actgctacgg cttccaacac gcccacggaa acggttacgc caacgcctga gggtcccttt
     3961 gtctatgagg ttgtgcaagg cgacaccctg tcctcgattg ctgaaaaatt cggggtggat
     4021 ttgtttgcct tgatcgccgt caataacctg gacccgactt tgccgattga catcggagac
     4081 cagctcacga tccctggctc cgacaccgag ttgccaacag agacgcccat cccgttgagc
     4141 atgcggcgcg gcacgatcat cgaatacatt gtcaagccag gtgagaatct ggcctcaatc
     4201 gccgaaaagt tcaacagcac cgtcgatgac atagtctcgg aaaacgagat cgaaaatccg
     4261 aatgaaatac aggctggcac caagttgaat gtacccgtca atctggtgac acccctcccg
     4321 accaatacag ccggtccctc gcccacagct accaccgcgg tgacaccggc cgatacgcct
     4381 accctgacag ccacgccata gcgatgtatc ctccactctg atagatatgc ccagttctgc
     4441 tgcctgtttg gctgacagca gaactggaat cctctgtttc tccatccagc ggataagtag
     4501 attggcagag aaagacttac cctaaaaaaa tatgggaata ttaagagagc ttcgcacatc
     4561 ttcgatatcc ggctaccatg ttccgcacca ggcgggcgct cccattctgg aaatctcaca
     4621 tctcacggtg cgctacgaga atgtgtttgc
//
LOCUS       DBNGSX010000342         5516 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1136683, whole genome shotgun sequence.
ACCESSION   DBNGSX010000342 DBNGSX010000000
VERSION     DBNGSX010000342.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1136683"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..662
                     /locus_tag="AC16CB_10225"
     CDS             51..662
                     /locus_tag="AC16CB_10225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR3342886.1"
                     /translation="MSSNQDYQDFIRSRRSVRRFLPDRVPQELLVRVLQTAAWAPSAH
                     NRQPWRFVELDSALARERLALAMGQDYQRDLHADGASEEQIQAQLQRSRQRITEAPAA
                     ILLCLETDEIDRYPDEHRQHAEYLMAVQSVAMAGQNLLLAAHAEGLGGVWMCAPLFAQ
                     EAARGALNLPESWQPQALVLLGYPAKVPPPRIRREMDEVVLHV"
     gene            717..1727
                     /gene="cofD"
                     /locus_tag="AC16CB_10230"
     CDS             717..1727
                     /gene="cofD"
                     /locus_tag="AC16CB_10230"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HIR3342887.1"
                     /translation="MKAEPLASLKVVALAGGVGGAKLADGLAQILPSNNLAVIVNTGD
                     DFDHLGLRICPDLDTVCYTLAGIANPATGWGRSGETWNAMESLAILGGPQWFRLGDLD
                     LGTHLERTRRLQLGDRLSQITRDFCRAWGVQLTVLPMSDDAVATWVYTDAGVLPFQEY
                     FVRQRCQPQVRGFRFDGVERALPAPGVLDVLESADVVVICPSNPWVSIEPILSVPGIR
                     EAVAAKFTVAVTPIIAGRAVKGPAAKMCTEMGIEPSAVSVAQHYSSLLDAFVMDQVDV
                     VQFEALNAQGMPTLATNTLMTSADDRRRLAKEVIEFGMHMLEKKPPKRHIRNIGFTQV
                     KE"
     gene            1795..2397
                     /gene="cofC"
                     /locus_tag="AC16CB_10235"
     CDS             1795..2397
                     /gene="cofC"
                     /locus_tag="AC16CB_10235"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HIR3342888.1"
                     /translation="MTLWAIVPVKPLRRGKSRLAEVLTQEERKDLNCNLLAHTVDTLA
                     AIPDIEHVLVVSRDPEALALAREHGARTVQENGASGLNIALAKATIVAKNYTTRGVLI
                     VPADLPLITPEDVRAILERVKDPPVVVVAPDQRREGTNALLICPVGLIDYNFGPKSFE
                     RHCELAVQAGARLEICELPSLALDVDLPEDLDAVGREFES"
     gene            2413..3408
                     /locus_tag="AC16CB_10240"
     CDS             2413..3408
                     /locus_tag="AC16CB_10240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR3342889.1"
                     /translation="MKRVALYLQDAHDLRDGLDYVRYAEQRGFEAVWQAESRLVRDAI
                     VPMAAYASVTERIKVGSGVINNWTRNIGLLAATFLTLDDLAPNRIICGIGAWWDPLAR
                     NVGIERRKPLLAMRETVEVLRRLLKMERVTFHGEFHQVDGIELDVVHGRREPRHVPIM
                     IGATGDKMMEMTGEIADGVVLNYCVPPEYNDNAMELLEAGAKRAGRKLDDIDRPQLVV
                     CSVDHDRERAIDTTRELLTQYLAQQPHIAKASGVSEDVVKEIQAILGWPATKEQIRRA
                     KHLVPEELIHRITASGTPDEARAKVQEYINHGATCPILYPVGGDVRLLVDTFAVV"
     gene            3405..3878
                     /locus_tag="AC16CB_10245"
     CDS             3405..3878
                     /locus_tag="AC16CB_10245"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR3342890.1"
                     /translation="MRQPNSRHCFVCGLANPHGLQLRFEITAPGEVVANYTVPERYEG
                     YPGVVHGGIVAAMLDEVVSRVHMGIDPPRFMYTARLELRYRKNVPIGQPLRIVGRAEK
                     SKGRTATSKGAIYDQNGDILAEAEALLVNVPDEMLSDVDLRALGWRIYDDEEIRG"
     gene            3945..4691
                     /locus_tag="AC16CB_10250"
     CDS             3945..4691
                     /locus_tag="AC16CB_10250"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3342891.1"
                     /translation="MVIEYNRPKTLDEALSLLSRKGIKTLPLGGGTILNQPSTERYAV
                     VDLQSLGLDTIQARGNTLTLGATVTLQKALESGRLPDGLNKVIRHEASYNLAGTLVSA
                     DGRSPFATALLALDAALEIKSASDPSERISLGDLLPVRGEHLAGRLITSVTLPLNIQL
                     VYEYVSRTPEDLPIVCVALARWPSGRTRLVLGGFGEAPLLALDGPEAGGAEVAARDAY
                     SRAGDEWASAEYRQDVAAILTRRCLSTITG"
     gene            4732..>5516
                     /locus_tag="AC16CB_10255"
     CDS             4732..>5516
                     /locus_tag="AC16CB_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3342892.1"
                     /translation="MEISLNVNDAVHTLDVPAGYTLLKVLRHLGYFGTKHGCETGECG
                     ACTALLDGKPVNSCLVLAAQAQGHRIQTIENIGQHPEQGWKLTEGLHPLQQAFVESGA
                     IQCGFCTPAQILAAKELLDRNPTPSEAEVREAFSGVLCRCTGYLKPVQAVLRAAAAMR
                     GEQVENGELVGFILPESRDGEPFGEATSSPIPYPLSPATSVQALPKVFVAPETETWRR
                     VGKPEIKVDAVKLVQGKPAFAADMDARGLLYAKVLHSPHAHAR"
BASE COUNT         1197 a         1402 c         1672 g         1245 t
ORIGIN      
        1 aagtttagac gctcgagtgc ctcgctgagc aaccggaggc ggcagctcgg gtgagttcaa
       61 accaggacta tcaggatttt atccgctcgc gccgttctgt gcgccgtttt ttgccagaca
      121 gggtgcccca ggaattgctg gtgcgcgttt tgcaaacagc tgcctgggca ccctcggccc
      181 acaatcgcca gccctggcga ttcgttgagc tggatagcgc attagctcgg gagcgccttg
      241 ccctagccat ggggcaggac tatcaacgcg atttgcacgc ggatggcgca tctgaagagc
      301 agatccaagc acagctgcaa cgctcacgtc agcgcatcac tgaggcaccg gcggcaatct
      361 tgctctgcct ggagactgat gaaatcgatc gctacccaga cgagcaccgc cagcacgcag
      421 aatatctgat ggcggtacag agcgtggcaa tggcggggca aaacttgtta ttagctgctc
      481 acgcggaggg tttgggaggg gtttggatgt gcgccccgtt gtttgcacag gaggcggcgc
      541 ggggcgccct taaccttccc gagagctggc agccgcaagc cctagttttg ttaggttatc
      601 cggccaaagt cccaccgcct cgcattcgaa gggagatgga tgaggttgtc ttgcacgttt
      661 gatcgatcct ggtttgaaaa gatcaaccga gcccgtattg gcggtctggt gagctggtga
      721 aagctgaacc gcttgcctcg ctcaaagtcg tggctctggc tggcggagtg ggtggagcca
      781 agttggcgga tgggcttgcg caaattctgc catcgaacaa cctggccgtg atcgtcaaca
      841 ccggtgatga ttttgatcac ctgggtctga ggatttgccc tgaccttgat acggtatgct
      901 ataccctggc agggattgcc aatccagcca ccggctgggg gcggtctggc gagacctgga
      961 acgccatgga gagtttggca atactgggtg gtccacaatg gttccgcctg ggagatttag
     1021 acctggggac acacttggag cgcacccgcc gcctgcagct tggcgaccga ctcagccaga
     1081 ttacgcgtga tttttgccgt gcctgggggg tgcagctaac agttttgcca atgagcgatg
     1141 acgctgtggc gacctgggtg tatactgatg caggcgtgtt accctttcag gaatacttcg
     1201 tccgtcaacg ctgccagccg caggtgcgag gttttcgctt cgatggtgtt gagcgcgcac
     1261 tgcccgctcc cggggttttg gatgtcttgg aatccgctga tgtagtggtt atctgcccat
     1321 ccaacccgtg ggtgagcata gagccgattc tttcggtgcc gggtatccgg gaagctgtgg
     1381 cggcaaagtt tactgtggcg gtgacgccga tcatcgccgg gcgcgccgtc aaaggtcccg
     1441 ctgctaaaat gtgtacagag atggggatcg agccttctgc cgtctcggta gcacagcatt
     1501 atagctcgtt gttggatgct ttcgtgatgg accaggtgga cgtagttcaa tttgaagcgc
     1561 taaacgccca gggaatgcca accttggcga caaatacatt gatgacctca gccgacgatc
     1621 gccgacgtct ggccaaagag gtgatagaat ttggaatgca catgttggag aagaagccgc
     1681 ccaagcgcca tattagaaat ataggattta cacaagtgaa ggaataaagc taaagtcact
     1741 tctctgcctt cctgctgcgt aatttctata tgattgagaa aggatcactg atgcatgaca
     1801 ctatgggcca tcgtaccagt caaacctctg cgacgaggta agtcgcgctt ggcggaagta
     1861 ctcactcagg aggagcgtaa agaccttaat tgcaatttgc tggcgcatac ggtagatacg
     1921 ttggcggcta tcccagatat tgagcatgtg ctagtcgtca gtcgagatcc tgaggcgctt
     1981 gccctggctc gagagcatgg cgcgcggacc gtgcaagaga atggggcgtc gggtttgaac
     2041 atagccctag ctaaggctac aattgtggct aagaattaca ccacgcgtgg cgtgctgatc
     2101 gtaccagctg atctacctct tattacccca gaagatgttc gcgccatttt agagcgtgtc
     2161 aaagatcccc cagtggtggt tgtggcgcca gatcaacgcc gggaaggcac gaatgctctg
     2221 ctgatctgcc cggtaggatt aatcgattac aattttggac caaagtcatt tgagcgacac
     2281 tgcgagctcg cggtgcaggc tggagcaagg ttggaaattt gcgaattgcc atctctggct
     2341 cttgatgtag atttgccaga agacctggat gcggtaggga gagagttcga atcctagtaa
     2401 ggagtttatg gaatgaagcg agttgccctt tatctgcaag atgcccatga tttgcgtgat
     2461 gggctggatt atgtgcgcta tgccgagcag cgcgggtttg aggctgtgtg gcaggcagaa
     2521 tcgcgcttgg tgcgagatgc gattgtgccg atggcagctt atgcgtcggt cacagagcgt
     2581 atcaaggtgg gctcaggcgt aattaacaat tggacccgca acatcggatt gctggcagcc
     2641 actttcttaa ccctagacga cctggcacca aaccgcatca tttgcgggat tggtgcttgg
     2701 tgggatcccc tggcacgcaa tgtgggtatc gagcggcgca aaccactgct ggcgatgcgc
     2761 gagacggtgg aagtgctgcg ccgcctgctg aaaatggagc gggtgacctt tcacggcgaa
     2821 tttcatcagg tggacggcat cgagctagac gtggttcacg gacggcggga accacgacac
     2881 gtgcccatta tgattggcgc cactggcgat aaaatgatgg agatgaccgg tgaaatcgcg
     2941 gacggcgtgg tgctaaacta ctgcgtgccc ccggaatata acgacaacgc catggaatta
     3001 ctagaggcgg gcgccaagcg cgccggtcgt aagttggatg atatcgatcg cccgcaactg
     3061 gtggtctgct cggtggacca tgaccgcgag agggctattg ataccacgcg tgagctgctc
     3121 acccaatacc tggcgcagca gccccacatc gccaaggcct cgggtgtttc ggaagacgtg
     3181 gtgaaggaga tccaggctat tttgggttgg ccagccacca aagagcaaat tcgccgcgcc
     3241 aagcacctgg tgcctgaaga acttatacac cgcatcaccg cttcaggcac acctgatgaa
     3301 gctcgcgcca aggtacagga gtacataaac catggcgcca cttgccccat cttgtacccg
     3361 gtgggtggtg acgtgaggct attagttgat acctttgcag tagtatgaga caacccaact
     3421 cgcgtcactg ttttgtctgt ggcctagcaa atccgcatgg cttgcagttg cgctttgaaa
     3481 ttactgcacc tggtgaggtt gtagcaaact atacggtccc agaacgctac gagggctatc
     3541 ctggcgtggt gcacggtggt atcgtggcag ccatgctaga cgaagttgtc agccgtgttc
     3601 atatgggcat cgacccgccg cgctttatgt acactgcccg cttggaattg cgctaccgca
     3661 agaatgtgcc gatcggtcag cccttacgca ttgttggcag ggcggagaaa agcaaaggcc
     3721 gcactgccac gtcaaagggt gccatctacg atcaaaacgg tgatatcctg gcggaagccg
     3781 aagccttgct tgtcaatgtg cctgatgaaa tgttgagcga tgtggatttg cgggcattgg
     3841 gttggcgtat atatgatgac gaggagataa gggggtagga gagagattat ccgtgcaaac
     3901 tgatttcgca tcttctttac tgctgggaat tggggagtca gtttatggtg attgaatata
     3961 accgtccaaa aacgctggat gaagcgcttt cgttactttc ccgtaagggt atcaaaacac
     4021 tgcccctagg tggggggaca atcctgaacc agccttcaac ggaacgatac gctgtggtcg
     4081 atcttcagtc tctgggttta gacaccatac aggcccgcgg caataccttg acgttaggag
     4141 cgacggtgac attgcagaag gcgctcgaaa gtggaagatt gcccgatggg ctgaataagg
     4201 ttatccgcca tgaagccagc tataacctgg cggggacatt ggtgtccgca gatgggcgtt
     4261 cgccctttgc cactgcgctg ttagccctag atgccgccct cgagatcaaa agcgcctctg
     4321 acccttccga gcggataagt ctgggggatc tccttccggt gcgcggggag cacctggctg
     4381 gacgcttgat caccagcgta acattgcccc tcaacataca attggtttac gagtatgtgt
     4441 cgcgcacgcc tgaagaccta ccgatcgttt gcgtggcatt ggcacgctgg ccgtctggcc
     4501 gcacgcgtct ggttctgggc ggttttggag aagctcccct tctggctctg gatggaccag
     4561 aagcgggtgg tgcggaggtg gctgcgcgag acgcgtatag tcgcgctggt gatgaatggg
     4621 ccagcgccga ataccgccaa gatgtagccg caatactcac cagacgatgt ttgtcgacaa
     4681 ttaccggttg aaggtggtga gttttaagca gaattgatgg ttggagatcg tatggaaatc
     4741 agtcttaatg tcaatgacgc agtgcataca ttggatgtcc cagcgggata tacactcctc
     4801 aaggtgctgc gacatttggg ctattttggc accaagcatg gctgcgaaac tggcgagtgc
     4861 ggagcttgta ctgcgttgct ggatggcaaa ccggtcaact cttgcttggt cctggctgct
     4921 caggctcaag gtcatcgcat tcagaccatt gaaaacattg gccagcatcc agaacaggga
     4981 tggaagctca ccgaaggtct gcatccgctt cagcaagcat ttgtggagag cggggcgatc
     5041 caatgcggtt tctgcacccc ggcccaaatt ttagctgcca aggagttgct cgatcgcaat
     5101 cctaccccta gcgaggcaga ggtacgcgaa gccttttctg gagtgttgtg ccgctgcaca
     5161 ggctatctca agcctgtcca ggcggtgctg cgtgccgcgg cggcgatgcg tggagagcag
     5221 gtagaaaatg gtgagttggt cggctttatc ttacccgagt caagagatgg ggaaccgttt
     5281 ggtgaggcca cctcatctcc aatcccttat ccactttccc cagccacttc cgttcaggcg
     5341 ctgccgaagg tatttgttgc cccagaaacc gaaacttggc ggcgggtcgg caaacctgag
     5401 ataaaggtag acgcggttaa gctggtgcag gggaagcccg ccttcgccgc cgacatggat
     5461 gcccgtggac tgctttacgc caaggtcttg cacagccccc atgcccatgc ccgcat
//
LOCUS       DBNGSX010000343         6092 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_999560, whole genome shotgun sequence.
ACCESSION   DBNGSX010000343 DBNGSX010000000
VERSION     DBNGSX010000343.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6092)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6092)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6092
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_999560"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="AC16CB_10260"
     CDS             complement(<1..507)
                     /locus_tag="AC16CB_10260"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR3342893.1"
                     /translation="MSEYVLTEQKNNLLVITLNRPKVNAFNLEMIKALQDALKQARRD
                     AQVRCVLLTGSGKVFSAGQDVTEVKEAEDTSFRYHLMRTYNPLILQIRQLEKPVLAAL
                     NGAVSGAAMGIALACDLRIAADTARFVVGFLGIGLGPDSAVSLLLPATIGLGRATEYA
                     FTNAPINAE"
     gene            complement(632..1015)
                     /locus_tag="AC16CB_10265"
     CDS             complement(632..1015)
                     /locus_tag="AC16CB_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342894.1"
                     /translation="MDEKITIIEGPPPTFETVADGWVLGLNEGPKLGNIAVTRLRTFN
                     GPALVERCYRAWHNQQTILLEYRSSDGLTQTTPIVAARHMELEDGQLLELWVRLTNDE
                     VQLELGYEDDLGDDMDDDLDNEDLL"
     gene            1162..1500
                     /locus_tag="AC16CB_10270"
     CDS             1162..1500
                     /locus_tag="AC16CB_10270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HIR3342895.1"
                     /translation="MNKLVILIEPPDDWEVFDNTWPQFLHHAENMPGLRREATSRVDT
                     FLYGERTYALMHELFFDSLDAAQQAMASPEGRQAGRILQEMSRGRMVLFFADHKEDDL
                     ENIKKMKGDR"
     gene            1532..2287
                     /locus_tag="AC16CB_10275"
     CDS             1532..2287
                     /locus_tag="AC16CB_10275"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3342896.1"
                     /translation="MSEFKGKVVLVTGAGRGIGRALAMAFAAQGARVAANDLTPVNLD
                     ETLKRITAEGGECKDFVFDVAGKMPVQAMIEEIRDEWGRIDILVNNAGVEPHASLLEM
                     DEWDWRRTLDVNLTGPFFTMQSVGRVMREQGGGIMVNIASIAGRAHGLKDRSAYVASK
                     MGLIGLTREAAREFAAYNIRVNAICPGVIETEMTAALRQDEAMLRKWLEDIPQGRLGR
                     VDDVVGLTLFLCSEAAAYVTGQAINVDGGKVMC"
     gene            2353..3603
                     /locus_tag="AC16CB_10280"
     CDS             2353..3603
                     /locus_tag="AC16CB_10280"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3342897.1"
                     /translation="MTLHIPPSLQHRRFRLLWLGLMISVAGAQMQVWALFWHIRTLTD
                     QPIALGGVGLARILPVIVFSLLGGAVADVANRRRVLFITQGSIGLIALVLAILTLNGN
                     IALWQIYLLTALQAVAVSFDQPARQALVPNLVPARDLPNAFSMMSIAFQTGAIVGPAL
                     SGLIIAYWGQPYTYLINAASYLAVILALILLGPVSQQTAVGRDARINLPAIREGVQFI
                     LSQPIILSSMILDFFATFFSSANTLMPIVARDILHVGAVEYGWLSAAQSVGAVTAALA
                     ISQMSAIRRQGPIFLGAVVVFGAATVVFGMVRGFLLAFFALMVIGAADAVSTIIRNTI
                     RQLHTPDYIRGRMTSVNQIFFMGGPQLGEVEAGVVAQLFGAPFAIISGGLGCILAVTW
                     IARRWPQLRAYNGDEPLRAGAPAD"
     gene            3620..4009
                     /locus_tag="AC16CB_10285"
     CDS             3620..4009
                     /locus_tag="AC16CB_10285"
                     /inference="COORDINATES: protein motif:HMM:NF019928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HIR3342898.1"
                     /translation="MDKDFIVSDVIHASPEEIYAAWLDSKGHSQMTGSPARASDQVGD
                     TFEAWDGYILGRNLALEPGKRIVQAWRTTEFGPSDEDSLVEITLKPAASGTRVTIHHS
                     NLPENGDQYRQGWIDFYFQPMKAYFKA"
     gene            4043..4939
                     /locus_tag="AC16CB_10290"
     CDS             4043..4939
                     /locus_tag="AC16CB_10290"
                     /inference="COORDINATES: protein motif:HMM:NF016840.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3342899.1"
                     /translation="MHEDKALWSDTLVHLMALAAKLEGEGQYNNAKLARAAAESMTRQ
                     EAYELSLPSERGQLVGEIQKAVSALTSLGADPGLLDAFRLGIDAMAAGRLPLIDETPH
                     PYVCRTCGRVELAQPTKICPTCGAWPATFKQFLPVYWLDALEPFAALERLRQTPIDVA
                     TLLKGMPEELLSREPDDGGWAIRNIVSHLRDAQGVLSFRLNLLLEQDDPVLESKAVFE
                     WAKKEEDRHPTTQEIFDTYNASRQEIVARLEAIPLSHWWRTGRHEEFGTVTLRQQVSY
                     FASHEATHLPQIEFLRDKWVGK"
     gene            4929..5978
                     /locus_tag="AC16CB_10295"
     CDS             4929..5978
                     /locus_tag="AC16CB_10295"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3342900.1"
                     /translation="MASSDFSIRLIAPGDGPALEALLKASPVRGEIGFTYDYQADILA
                     VSQALAVDLVGAVAVQDGAVIGMVYGELLPVQWAGGVCQGVYVSNLRVRPDFQRQGLA
                     RRLGDWGLAYLTERLGRDVVLYAAIPEGNISLNLAQRHGFISTRQIQGGVIPMRRTPP
                     RLIPGLQIRPASREDLPAFAEGVNEFYREHDLWNPVTPASLEIFLERQVRGIRPNQLY
                     IVTRGGEVVGGLSLSDRTQLVRMRVTGASAFVRLLGDLLGVLPRDGVLRALTVREVWF
                     GDGQLEAGRYLWQQLRYRLRERGDCLGIAYDPHDRLADLFQVPFWLPLVKARYLVRAG
                     SLPEQGRPIYCIAGA"
BASE COUNT         1251 a         1572 c         1851 g         1418 t
ORIGIN      
        1 ttctgcattt atgggcgcat tggtgaaggc gtattccgtt gctcgcccca agccaatcgt
       61 cgcaggcagc aacaatgaga ctgccgagtc agggcccaag ccgataccca gaaacccaac
      121 cacgaagcga gcagtatcag ccgcgatgcg aaggtcgcat gccagagcga tccccatagc
      181 ggcaccggaa acagccccat tgagagctgc caggacaggt ttctccaatt ggcgtatttg
      241 caggatcaac ggattgtagg ttcgcattag gtgataacgg aaggatgtgt cctcggcttc
      301 cttcacctcg gtcacatcct gcccggcgct gaataccttt ccgctgccgg ttagcaaaac
      361 acatcgcacc tgggcgtcgc ggcgggcctg cttgagggca tcttggaggg ccttaatcat
      421 ctccagattg aaggcgttaa cctttggccg gttgagcgtt atcaccaata ggttgttttt
      481 ctgttcggtt agcacatatt cagacatagt acctccaatc caggcgattg acacgagttt
      541 agcacaattt atcccaacct gaaagaaaaa attgagggat gtgggtggtt gttcccgcat
      601 ccctcaattt atgtgattaa tctgtactgg tttaaagcaa gtcttcgtta tccaggtcgt
      661 catccatatc atcccccaga tcgtcctcgt accccagctc taattgaacc tcatcgttgg
      721 tcaggcgaac ccatagttcc agcaactggc catcttcgag ctccatgtgt cgggcggcga
      781 caatcggggt agtctgcgtc aacccatctg aggagcgata ttctagcaga atggtctgct
      841 ggttgtgcca ggcgcgataa caacgttcca ccagggctgg gccattgaac gttcgcaggc
      901 gggtgacagc gatattgccc agctttggac cttcattcaa accgagtacc catccatcgg
      961 caacagtttc aaatgtcggc ggagggccct caatgatggt gattttttca tccatagatt
     1021 tgcctctaat ctgtcgaatc ataccacaat ttagttttaa taatgcttac gcaattgtac
     1081 aaaatagaga tgtgtgaagg atcacttctt tccctcgcca actgcgtagc ttgtgtatat
     1141 aatcaaggcg gagacgtcaa gatgaacaaa ttagtaatat tgattgagcc tcctgatgat
     1201 tgggaggtat ttgataacac ctggcctcaa ttcctgcacc atgcggagaa tatgcctggt
     1261 ctgcgacgcg aggcgaccag ccgcgtggat acattcttgt atggagagcg cacctatgct
     1321 ttgatgcacg agttgttctt cgactctttg gatgcagccc agcaggccat ggcttcccct
     1381 gaaggacgcc aggctggcag aatcttgcaa gagatgtcgc gcgggcgcat ggtgctgttt
     1441 ttcgccgatc acaaggaaga tgacctggaa aacatcaaga agatgaaggg tgatcggtga
     1501 aggcactagt cgtagtttta attgaggaga tatgagcgaa ttcaaaggga aagttgtcct
     1561 ggtcactggc gccgggaggg gtattgggcg agcgctggca atggcattcg ccgcgcaggg
     1621 cgcccgggtg gctgctaacg atctcacccc ggtgaatctg gatgagacgt tgaagcgcat
     1681 cactgctgag ggtggggaat gcaaagattt tgtcttcgat gtggctggca agatgcctgt
     1741 gcaggcgatg atcgaggaga ttcgcgatga gtgggggcgc attgacatcc tggttaacaa
     1801 cgcgggtgta gaaccgcacg cctcccttct ggagatggat gagtgggatt ggcgccgcac
     1861 actggatgtg aacctgaccg gtccattttt caccatgcaa tcggttgggc gagtgatgcg
     1921 tgagcaaggt ggaggaatta tggtcaatat cgcctctatc gccggtcgag cacacggttt
     1981 gaaggaccgc tctgcctatg tggccagcaa gatggggctg attgggctga ctcgtgaagc
     2041 tgcccgcgag ttcgccgcct acaatatccg tgtgaacgcc atctgtccgg gggtgattga
     2101 gacagagatg acggctgcat tacgccagga cgaggccatg ctgcgcaaat ggttggaaga
     2161 catcccccag ggtcgcttgg gacgggtcga tgatgtggtc ggtctgacgc tgtttctgtg
     2221 cagtgaggca gctgcctatg taactgggca ggcgattaat gtggatggtg ggaaagtgat
     2281 gtgttaaagt ctcgcattcc ggatatgtgc attgcataat aaatattccc tacaccgata
     2341 actaatagcc ctatgacgtt acatatccct ccttctttgc agcaccgcag attccgtttg
     2401 ctctggctgg ggttaatgat ctctgttgcc ggtgcccaga tgcaggtatg ggcgcttttc
     2461 tggcacattc gcactctcac cgaccaaccg atagccctgg gtggagtggg attggcgcgt
     2521 atcctgccgg tgattgtatt ctcgcttttg ggtggggccg tcgccgacgt cgcaaaccgg
     2581 cgcagggtgc ttttcatcac ccagggttcc attgggctga tcgcccttgt cctggctatt
     2641 ttgaccctca acggaaacat cgctttgtgg cagatctact tgttaaccgc gcttcaggca
     2701 gtggcagtat cctttgacca gccggcgcgc caggcactgg tgccgaattt ggtgcccgct
     2761 cgtgatctac ccaacgcgtt cagcatgatg tccatcgcct ttcagaccgg agctattgtc
     2821 ggaccggcgt tgagcgggct gataatagca tactggggac aaccatatac ttacctgatc
     2881 aacgcggcct cttacctggc ggtgatcctg gcgttgatct tactgggacc cgtctcgcag
     2941 caaaccgctg tcggtcggga tgccaggatc aacctgccgg cgattcgaga gggtgtgcag
     3001 ttcatattga gccaacctat catcctctcc agtatgatat tggacttttt cgccaccttt
     3061 ttctcctcag ccaacacttt gatgcccatt gtggcgcgcg atatccttca tgtaggtgct
     3121 gtggaatatg gatggctctc agctgcgcaa tcggtggggg cagtgacggc ggcgctggct
     3181 atctcgcaaa tgagcgccat ccgccgccag gggccgatct ttttgggggc ggtggtggta
     3241 ttcggtgcgg caacggtcgt ctttgggatg gtgcgtggtt ttctgctcgc ttttttcgcc
     3301 ctgatggtca ttggtgcggc ggacgcggtg agtaccatta tccgcaacac catccgccag
     3361 ttgcacacgc cagattatat ccgcgggcgc atgaccagcg tgaaccagat atttttcatg
     3421 ggcggtccgc agctgggaga ggtggaagca ggggttgttg cgcagctatt tggcgcgccg
     3481 ttcgccatca tcagcggcgg gttggggtgc atcttagcgg tgacctggat tgcccgccgc
     3541 tggcctcagc tgcgcgcata taatggagat gagccgctca gggcgggtgc cccagcagat
     3601 tagcgctatg gagtaggata tggacaaaga ttttatcgta tcagatgtga tccatgcatc
     3661 accagaagag atatatgctg catggctgga ttccaaaggt cactcgcaga tgacgggcag
     3721 ccctgccagg gcgagcgacc aggtgggcga tacgtttgaa gcctgggacg ggtacatcct
     3781 gggtagaaac cttgccttgg agccgggcaa gcgcattgtg caagcctggc ggaccacgga
     3841 gtttggacct tctgacgaag attcgcttgt cgagatcacc ttaaagccag cagccagtgg
     3901 gacgagagtg acaatacacc attccaatct gccggaaaac ggcgatcagt accggcaagg
     3961 gtggattgat ttctactttc agccgatgaa ggcttatttc aaagcgtgag aagccaaata
     4021 gtgattatct ggaggtggtt ttatgcatga ggataaagcg ttgtggagtg atactttggt
     4081 ccatttgatg gccctggcag ccaagctgga gggtgaaggg caatataaca acgccaagct
     4141 ggcgcgcgcg gcagctgagt ccatgacgcg ccaggaggcg tacgaactta gcctgccgtc
     4201 tgagagaggc cagctggttg gtgaaatcca aaaagcggtt tcggcgctca cctcgctggg
     4261 ggcagacccg ggcttgctgg atgccttccg ccttggcata gacgccatgg ctgccgggcg
     4321 actgcccctg atcgatgaga cgccccaccc gtatgtttgt cgcacctgcg ggcgcgtcga
     4381 gcttgctcaa ccaaccaaaa tctgcccgac ttgcggggcg tggcctgcaa ccttcaaaca
     4441 attcttgccg gtctactggc tggacgccct ggagcccttt gctgcgctgg agaggctgcg
     4501 ccagacgccc atcgacgtcg ccacgcttct gaaagggatg ccagaggagc tcctcagccg
     4561 ggagccagac gacggtggtt gggctatccg gaatatcgtg tcccatttaa gggatgctca
     4621 gggggtgctg agcttccgct tgaatttgct gctcgagcag gatgaccccg tgctggaatc
     4681 aaaggcggtc ttcgagtggg cgaagaaaga agaagaccgt catcctacta cgcaagaaat
     4741 tttcgatacc tacaatgcct cccgacagga gatcgtcgct aggctggagg ccatccctct
     4801 gagtcattgg tggcgcacag gccggcacga ggagtttggc acggtcacgc ttcgccagca
     4861 ggtgagttac ttcgcctctc acgaagccac ccacctgcct caaatcgagt ttttgcggga
     4921 taagtgggtt ggcaagtagc gacttcagca tccgcctgat cgcaccgggg gatggtccgg
     4981 cgctggaagc actgctgaaa gccagcccgg tgcgcgggga gatcggcttc acctatgact
     5041 atcaggcgga catcctggcg gtcagccagg cactggccgt tgatctggtc ggtgcggtgg
     5101 ctgttcagga tggtgcggtg atagggatgg tgtatggcga gctgctgcct gtgcagtggg
     5161 ccggtggggt gtgccagggc gtgtacgtat ccaatttgcg ggtacgacca gattttcagc
     5221 ggcagggttt ggcgcgccgg ttgggggatt gggggttggc ttaccttacg gagcgccttg
     5281 ggcgggatgt agtcttgtac gcggccatcc ccgagggcaa tatcagcctc aacctggcac
     5341 agcgtcacgg atttatctcc acgcgacaga tccaaggagg cgtgatcccc atgcgccgta
     5401 ctccgccccg actgatccca ggcttgcaaa tccgacctgc cagcagagaa gacctgcccg
     5461 ctttcgccga gggggtaaac gagttttata gagaacacga cctgtggaat cctgtgacgc
     5521 ccgcctcctt agaaatattt ctagagcggc aggtgagggg tatccgaccg aaccagctct
     5581 atatcgtgac gcgcggcgga gaagtcgtgg gcggcctgag cctgtcggat cgcactcagc
     5641 tggtgcgcat gcgggtcacg ggtgcctccg cctttgtgcg gttgctgggc gatttgttgg
     5701 gcgtgctacc aagagatggg gtgctacgcg ccctgaccgt gcgtgaggtc tggtttgggg
     5761 atggtcaact ggaggccggg cgctacctct ggcagcagtt gcgctaccgg ttgagggagc
     5821 gaggggactg tttgggtatt gcctacgacc cgcatgatcg gctggcggat ttattccagg
     5881 tgcctttctg gctcccgctg gtgaaggcgc gctacctggt gcgggcgggc agtttacctg
     5941 agcagggacg tccaatttat tgcatcgccg gggcctaggt ctagtgatgt aaaccaggcc
     6001 agcaagctgc tggttgaaaa gagacatctt catttatcat ccactgtagg ggtgaaccct
     6061 gctctttgga gccagggcaa ttgcatctgc cc
//
LOCUS       DBNGSX010000344        25808 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_791430, whole genome shotgun sequence.
ACCESSION   DBNGSX010000344 DBNGSX010000000
VERSION     DBNGSX010000344.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 25808)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 25808)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25808
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_791430"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="AC16CB_10300"
     CDS             complement(<1..298)
                     /locus_tag="AC16CB_10300"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR3342901.1"
                     /translation="MTHLIFIRHGETDWNTEGRWQGQIDVALNPKGRQQAQDLAEKLS
                     GANLQAIYSSDLQRAIDTARPLAEMTGLTIRTDARLREIHQGEWQGMLVSDIRAR"
     gene            complement(295..2106)
                     /locus_tag="AC16CB_10305"
     CDS             complement(295..2106)
                     /locus_tag="AC16CB_10305"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HIR3342902.1"
                     /translation="MRKYTAPAFRKAMVPIIAGCESKDAIRAAGAVSGLGEVVMLGLV
                     AAAPDEPLSASAEQARQVRKMMREFAKKDRFRSLGRVRVSNSPSEELFEAVIDEEPDL
                     LLIEWPRQHEALGISLNEAMQRCTCAVALVRGKIPEKPHHILIPIRGGPFAELSLRIV
                     LAISRNTRASVTALHIIPPSSNYIQEAPFKGLSHVLSNLPEVKYHEIHTDDPVQAILE
                     ASHGFDLVVLGATAQVSPKQEFLGPVAERILNECSAGVMVVKTPRSMPVDMGSELIGQ
                     TAISVLVDKWFAENTYHADEFSNLKELLALKRKQGVTISLALPALNEEETVGNVIQTV
                     KSVLMDEYPILDEIVLMDSNSTDQTREIAKDLGVPVYIHQEILPQYGARRGKGEALWK
                     SLYTTRGDILVWIDTDIVNIHPHFVFGLIGPLLANPKLQFVKGFYRRPIKVGNRIQAG
                     GGGRVTELTARPLLNLFYPELSGLIQPLSGEYGGRRKTLERLPFSSGYGVEIGLLIDV
                     LENFGLDTIAQVDLRKRVHHNQPLESLSKMSFTIIQTVIRKVEWRYGRNILEEVNKTM
                     KLIRYQSKRFFLEVEEIVERERPPMVDVPEYRELHSL"
     gene            complement(2202..3431)
                     /locus_tag="AC16CB_10310"
     CDS             complement(2202..3431)
                     /locus_tag="AC16CB_10310"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3342903.1"
                     /translation="MKVDLLHYSSPPIVGGVESVMAHHARLMADAGHEVRILAGRGDV
                     FDERVQFVNIPFVDSRHTNILEIKRDLDQGNIPPQFPILKDQLVSTLKEITLDTDVLI
                     AHNVCSLSKNLALTAALYQLSQERNSPRLILWHHDLAWTTPRYRSELHDGYPWDLLRR
                     DWAGAKQVVVSALRRQELAELLGIPLERVVVVPNGLDIERFLKLEERSVELVSQLDLL
                     GCAPLLLLPVRITPRKNIELALGVLAALRIQFPQAALVVTGPLGPHNPANIHYFNRLL
                     SLRESLGLKGCAHFLAEVSSEYLPDGVIADFYRLADALFFPSREEGFGIPILEAGMTG
                     LPIFCADIPPLRELAGENALYYDPDSAPEVVASMISDHFASNPIYDLRVRVKKHYTWQ
                     QLYVEQIEPLLTEVVSL"
     gene            complement(3421..5211)
                     /locus_tag="AC16CB_10315"
     CDS             complement(3421..5211)
                     /locus_tag="AC16CB_10315"
                     /inference="COORDINATES: protein motif:HMM:NF046269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HIR3342904.1"
                     /translation="MKPNAIELLKNRINLRQIPFSDRGSRILVFQEDDHFSVRLAERW
                     LKLAGKLSAYRQRPPLIEQWAFTDGDGNPMPLKLTTYPHCMIVDTPVGVFTMAFQDSE
                     TLYVSLPPARCGMDFYARLDQAHVDRRGGVLRLTGDIRRNMAYTTNAHIIENSAFAVE
                     GDGHRVKLRLDATEGNRGLLLNVTPRLGFNRWVSEPEPVLEASRQRWEEWFAAVPPVL
                     EEYEHQYYYAWWIMRVGLLSTRYYTTREVMTPSKSYYIGSWQWDNFFHALAYRYIDTR
                     LAQDQLRILMDHQREDGMIPDAIHDEGTVTHLDFPVEADVTKPPLVAWAAWKLYEHDH
                     DREFLNEIYGPIIKWNNWWFEKNDLDQNGLCEYQHPFSSGLDDSPLWDDGMPVDSPDL
                     NTYLFLQQEALAKIAVEIGEQEEGLMWSQRADAMAQRLIKLRWDKRAGVFWATHLGKR
                     VKVLTPFNLFPLITGKMPKEINERLVGHLTNPDEFWARYPVPSVALNDAKFDPWQMWR
                     GPTWANINFLLIEGLFRCGYDDLARELRRCTLDLIASLPDIYEYYHPISGENPPKAAP
                     IYGWSAALFIEMVIQATNEAKGADGEDYEG"
     gene            complement(5215..6483)
                     /locus_tag="AC16CB_10320"
     CDS             complement(5215..6483)
                     /locus_tag="AC16CB_10320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3342905.1"
                     /translation="MKPENYNIGFVSTRFRGHDGVSLEAEKWLEVLVRLGHNCYLFGG
                     DSDEYEKDYTFLVPEAHFKHPDIQEVYSIAFSALTRPPEVTRRIQTLKEHLKQRLNAF
                     VDQYKIRLLIVENALAIPLNIPLGLALTEFISETRIPVIAHHHDFFWERKRFLTNCVW
                     DYLNMSFPPHLPSIRHVVINSSASNQLSLRTGISAMTIPNVMDFDHPPQPPDDYTATF
                     RPDVGLNSGEYLFLQPTRVVQRKGIEHAIELIQRIGLKARLIISHASGDEGYEYEQRL
                     RYYSELMGVSAYFVSDIIRDSRGSLEDGRKIYTLWDAYQAADLVTYPSSTEGFGNAFL
                     EAIYYHRPIVVNNYSIFAIDIKPVGFKVIEFDGFITENTVKQVVGILEDRRLAEQMTE
                     HNYSLAQRHYSFNMLERRLNTMLMECFGEE"
     gene            complement(6647..7342)
                     /locus_tag="AC16CB_10325"
     CDS             complement(6647..7342)
                     /locus_tag="AC16CB_10325"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3342906.1"
                     /translation="MNRDSIHNQNNLADTRHRSSQSTQSHYDSLSRWYDLLTTWSERS
                     VRMAGLRMLDAQRGEAILEIGFGTGQAVLDLAKSVGDAGKVFGVDLSWGMVARARSKV
                     ASAGQSTRAALTHGDANKLPYKAEIFDAIFMSFTLELFNDEEIKAVLSECRRVLKKGG
                     RSCVIALLRKDHANIITRIYDWAHSHFPAAIDCRPIFVRNEILHAGFHIEQAVEMTLW
                     GLPVMLVIAKKTS"
     gene            7568..8455
                     /locus_tag="AC16CB_10330"
     CDS             7568..8455
                     /locus_tag="AC16CB_10330"
                     /inference="COORDINATES: protein motif:HMM:NF013539.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR3342907.1"
                     /translation="MQPELNSSEVNSQTLDERISALHKSLSPKHKRLARFVLDNKYFI
                     SFASASQAGEKIGTSAATVVRFAQTIGYEGYSEMQAAIRAELPSYMTAIVRMQVQLQA
                     KQPSNDLLRSVYQADINNIQRTVSNISEEKLNAALETLVNANRILVVGAGLSSSCAMF
                     LAHSLKIIGRDARVNSNEGLSLAADIALLNPGDLLIAIDMWRYVRSTIQAVSKAKEMG
                     VSVIAITDSIVSPLARLADYAFEVVTSGISHSLSATAIMSLLNVFIAALSYKIPEQTM
                     RSLRRVDSAYRDNDLLVLE"
     gene            8458..9642
                     /locus_tag="AC16CB_10335"
     CDS             8458..9642
                     /locus_tag="AC16CB_10335"
                     /inference="COORDINATES: protein motif:HMM:NF014381.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HIR3342908.1"
                     /translation="MNILICGGGNAAHALAGLLSTQEDLRVRVYLSFEDEARQWKEGL
                     AEQKSLHVHLPQGELVGRPVQVRSQAAEIVPGADLVLLALPAFAHEVVLRQMTPHLQP
                     AAWVGALPARGCFDLCALQTLGEKAPAVNIFGLQTLPWACRIERYGRSVRVLGTKAQV
                     DLASWPAELAPQIAATLSPLLGVDLQPVSSLLSLTLAGTGQLIHPGIMYGLFHNWDGQ
                     PYAQAPLFYQGIDPYTAGILQSMSDEVQELRHNLEARFSWLDLTAVRNLYDWLRFSYT
                     SDIADDTTLQSAFTSNRSYAGLQAPMRSVDGGLVPDFHARYISEDVPYALIATRGIAE
                     LAGVPTPQIDQVIRWAQGCLGQEFLVSGVLRGQDLGASRAPQRYGYQSINSLVESTRM
                     PV"
     gene            9711..13418
                     /locus_tag="AC16CB_10340"
     CDS             9711..13418
                     /locus_tag="AC16CB_10340"
                     /inference="COORDINATES: protein motif:HMM:NF025309.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HIR3342909.1"
                     /translation="MPTRNKSTQKDTKTSQKRRVMVGAIGKCVHNLGVENFADWMQDL
                     GTGYVSVKLGPAVPIREAVNKIREARPEVVGISMRLGDLHVDKLISEFIETATQYGLS
                     PRDSGIRYSFGGLRPAANLVRAMTGLPLEEDRFIRDEERHYDLAQVSEEYKKKEHFQG
                     FFELVADDFVSMEELEGFAQRRSTVTARKDAPWSDYLVERMRQVRERENRPIIRAHIG
                     IAADTIEPTIKAIEKLADAEAFEIVSLAPDQTSQELLAKFIRGEEDPSKYLAGQGGAP
                     IRTVEDLQRLKAATQRGNYPMTRIYTGTDELVALANLWEDNLNICFPAVPIFFYNELD
                     GRGPISIRDSFDEHFRTIEHWAKIGKPLEINDPHQWGLRYASDDMQVTDHVLCAVIAL
                     KKGIKNYVMQMMFELPPEISALDDLAKMRASYELAEPLARNFDFRIIRQTRSGLPSFP
                     PNLNQAKGHLAFGIYTQLYMQPDILHVVTHSEAHHEATADDIIESCEIVKQVCWDFAK
                     GNVPKIWSDREMKMRIKELKQGAMYNFLHCAILGGYDGPIRPENFWEWAKERREDREK
                     NYETMLLSLIDEANYPTGICGVIAGDTLDLALQIGLFQGPHITVVDRRYEMAGACRTK
                     VVNGMCRTEEWNGIKVFSEFERVDLVRKTYPWYFYKEVTRADEMSFIAEDAEVEKIDE
                     DAVNEFRKQIGITGLADEKVLVVDFGSTYTKVGILDTKKETFELRYKPTTVEDIRIGL
                     ANGLGVLEACRESGDWKPLAKKMREFAVRLPCSSAKGGLKMVTVALVKEESGFAAELA
                     ALTAGAKLVASYDGMLTAEQARQIYEVDQPEIILIAGGTDEGGDSSTQLHNARLLARE
                     SRYANYTDYGVPVIYAGNQDIRDRIRNIFRYRNIHLRLTPNVMPEINDFRIEVVNEAI
                     RELFQTVIIRGKGFDVVEEYMDAPFIPTPRAAFRGINLLANGYGNEEGLGNIMALDIG
                     GATTDFYSNVNDNPLYVFPGDDAQRKVKRTILKTPNVPLVFRRVEGKFGLSYNAENLK
                     ELESFKDGSMARDLSRYLDHRFVKEFAPGEDQFRRFIHRQNGSHQVDLDGYLSWISQN
                     PHQNPETPLENAATSYLAREIMTVTTGKHVGHVDETETYFLQYGVNYFNQPVTVLLIG
                     GTIYQKCRDRESGYLDDLMTIAEGVLYNPREDTSLRPKAPVLLDAKYLVSILGGLYGR
                     VNPEQALQVMKRELLPLDRIAQESSQAVLKS"
     gene            13547..15265
                     /locus_tag="AC16CB_10345"
     CDS             13547..15265
                     /locus_tag="AC16CB_10345"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3342910.1"
                     /translation="MSRKLSYFIRVLLPLAVILTACAQPPAAPPEEMIVKETVVVVET
                     VVVTEKEEVEKVITPTPVPGRQTIIVGLSQAPSEGLDPADHRSRLSETVIRNMFDGLV
                     TRDNRSGVHLELAEEMNWLDDVTLEVKLRQGVKFHDGVEMTADDVVFTFNRIIQENQI
                     EYPEPHTSPRKGLIAPLASIEKVDDYTVVMNFSDPWPPAMQGIVHQQIVPKHYLEEVG
                     TEGFVANPIGTGPFKFVSAQPGFEEVVLERFDDYYGGAPDLEPVGPACVSGVIFRTIP
                     EASTRVAALLAGEVDIIQSVPAELIDTLAQTPGIQVKTAAGTQPKWMEMNVNKPPFDD
                     VRVRQAMNYAIDKDLIIQEIYGGRAVALPGPLSPYNNFVNKSLVPYPYDPDMALSLLS
                     EAGWSDTNGDGLLDKNGEPFVFTIDTLEDWRSLAEAVADMYRAIGIDASVRFWEYSVV
                     KPQLQAGERMAYLDDWGDSAFDPFGHFEAKWHGLVEDTSYGRGNFSGYNNERVNELTK
                     QGEITIDIAARQAIYDEAQQIVYDEAPAVFLILPDEAEAASDRVTNWEPAADSRINLH
                     DVCLAP"
     gene            15369..16379
                     /gene="nikB"
                     /locus_tag="AC16CB_10350"
     CDS             15369..16379
                     /gene="nikB"
                     /locus_tag="AC16CB_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease"
                     /protein_id="HIR3342911.1"
                     /translation="MRAVKILERILATIPIVFGVAIVVFFFIRLTPGDPVDIMMGQGG
                     AVSEGEIEQLREEFHLDEPLHIQLWLFLKDAVRGNLGYSYVLKRPVTTLIAERLPATI
                     ELAVGGLILSLLIALPIGIISAVRQNSTLDRVSMASAFLGISMPGFWLGIILILFFSV
                     RLNWLPVQGRITFGTNLQEITGYFVLDSLLSGNRQALISSLKHLILPSITLGAPVAAV
                     VARVLRSSMLETLRSDYVTLARSKGATEWSVVLKHALRNALIPTVTVVGLQVGILLGG
                     NMVVETVFGWPGLGRMVVSAIFDRDFPLIQGSVMIYAFTFVMANLIVDVLYTYLNPKI
                     SL"
     gene            16438..17376
                     /locus_tag="AC16CB_10355"
     CDS             16438..17376
                     /locus_tag="AC16CB_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3342912.1"
                     /translation="MEVAPQVRTPFSHQVYVWRIRLAIWRQNAAEFLYELRKHPLALL
                     GGLVIVVYVVLAIFAGQVTVYDPVRGNLRLRLDPPAWEEGGSWEYPLGTDAQGRDLLT
                     RIIYGARVSLLVGFLSVGISVILGMITGTLAGYYRGWFDNLISRFADLLLAFPFLIFA
                     IGMMAFLGPGFINLILALTFKGWVEFFRLVRGEMLSEKTQEYVEAARVAGLSHLAIIA
                     REIIPNIIQSVFVLGTLRIGYMIIMEASLSFLGLGIPPSIPAWGSMVSAGRDYMLVAW
                     WVSTLPGIAILILVLAINLFGEGLRDILDPRLKIEG"
     gene            17400..17978
                     /locus_tag="AC16CB_10360"
     CDS             17400..17978
                     /locus_tag="AC16CB_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3342913.1"
                     /translation="MPKQALLELQNLTTVFPTRRGQVRAVESLNLELFEGEILGIVGE
                     SGYGKSTVLLSILRLIRQPGYIASGKIRYRGRDLRELSTPAMRSVRGKEIAMIFQDPQ
                     STLNPAFTVGEQIREALRLHHIVPGNPYPWPLDYVVRTREKKRVLEVMEEVGIPSPMD
                     RYASFPHQFSGGMQQRALIASAGMRASTAAGR"
     gene            18028..18453
                     /locus_tag="AC16CB_10365"
     CDS             18028..18453
                     /locus_tag="AC16CB_10365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR3342914.1"
                     /translation="MRRINQERGTALILVTHDLGLAAEFCSRIAVMYAGRIVEQGTVD
                     QMVENPQHPYTQGLLNCRPRISMRELRVEPIPGNVPDLAELPEGCAFAPRCAHVQPAC
                     QAGPIPMVSIKADRLSRCLLHIGYHREDAWKWDHTVNSP"
     gene            18450..19517
                     /locus_tag="AC16CB_10370"
     CDS             18450..19517
                     /locus_tag="AC16CB_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3342915.1"
                     /translation="MKEAQSESTPTPLFEGKGIYMHFQIRPSLLARALAGVNEKVVRA
                     VDGVDLAIWPGETLGLVGESGCGKTTLGRVLTRLYEPTRGQIYYRGQPVKGDTILEQQ
                     DGSNQTRRVNYHRLAQIIFQNPYSSLNPRKTVREIIGTPLQQRGVRDPLLREEETRQL
                     LQRVGLSKRHIDRYPHQFSGGQRQRIGIARSLAMRPKFIVADEPVSSLDVSIQAQVIN
                     LLEELQAEYHLTYLFIAHDLSVIYYISDRVAVMYLGHIVELGKTDYLFESPLHPYTQA
                     LLAAIPRVSKAARKERILLPGGVPSPIDPPSGCPFHPRCSAKVGPICEQEYPPFFQVN
                     NQQVACWLYQDHSIADQSSTK"
     gene            19539..20648
                     /locus_tag="AC16CB_10375"
     CDS             19539..20648
                     /locus_tag="AC16CB_10375"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HIR3342916.1"
                     /translation="MRQLYLSGDHFAMGKQHGHQVTDLKPHILSAIQHRLDKLAKVEL
                     NTGHLEEQMLESWHAQAEPLLEMMQGIAEALSLDWRVFFRYTIATFLMDMARQPQVGD
                     QGCTTWAVLSEATRDGIPLLVKNRDYWPDHQELQCLAHTRPDKGYVYLNMTSAGSPGV
                     FSSGINEAGLAVSDTHVSSLDIGPGLPRYYLMMEILEHHDTVHSAVDYLRSVNHAGNG
                     TLLLLDANGEAAVFECGQIFSKSIPATNGFVVSANHYVTPALRQKWMFRTTDRKLIGS
                     SLHRYSRVKRALLKAKGSIDLNWAINLMRQHGTPLNAICRHPELNPQSVTISSAIYLP
                     TKKQLYLVNGIPCQNEYQVYPVIPDVQDEKQAYAL"
     gene            complement(20751..23075)
                     /locus_tag="AC16CB_10380"
     CDS             complement(20751..23075)
                     /locus_tag="AC16CB_10380"
                     /inference="COORDINATES: protein motif:HMM:NF012544.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIR3342917.1"
                     /translation="MSTIAPNTTQSIPEKVTLSRSLGLFTITMIGVGGMIGAGIFVLT
                     GIAAGVAGPALILAFVLNGLVTILTGMVYAELGSAFPEAGGGYLWVKEGLGGAQGFLA
                     GWMSWFAHAVAGSLYALGFGRFFSELWVLAGVPTFNLEVEQMTLIFMTLIIAAFTYLN
                     YRGASETGTIGNIITMTKILILGVFVLFGVLAMLRTDAWHTRFTSGFMPNGMLGVIIA
                     MGLTFIAFEGYEIIAQTGEEVINPRKNVPRAIFLAIILVVVIYVFVGVTAIGAVTPPQ
                     GMKAYEYLGEQGEIAVVGVAAQIFPLGVGAVIMLVSGLVSTTSALNATTYSSSRVSFA
                     MGRDHNLPSIFARIHPLRHTPYLAVIASGALMLLIAWILPIEEVAAAADIMFLMLFLQ
                     VNLAVMTLRHKMPDLERGYKVPWHPFIPILALFSNAILALSLFSYAPVSWYTAIAWVI
                     FGLLIYYTYFSKIEAMEKPKEILMEEVLVTRDYSIVVPVATQEQAMILGQIGAILAKV
                     NNGEVLALHVVRVPRQFTLGEGRLFLKEGRSYLETVIHRAKPFDVPVHTVIRLGRSVA
                     EAVHKTIDESASDLLVLGWPGYTHTAGRIYGSVIDPIIDNPPTDIAIVRYRQRRSIKK
                     ILVPVAGGPNSRLAVKLAVDMAMSGEGGPALVSLLHIIPFGARNGAIVRAQQAFRESM
                     SGIAYENIEKRVVEGSNIIETILSEAAGDEDTPQSDLIVIGATLEPLLKKLRMGNISE
                     QVARRASVTVVIVKRRSGPLHSFLRQTVMEPTRI"
     gene            complement(23098..24819)
                     /locus_tag="AC16CB_10385"
     CDS             complement(23098..24819)
                     /locus_tag="AC16CB_10385"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HIR3342918.1"
                     /translation="MDKILAHFVYLYGDQEGRATYDRLVRLIDRYRSRIAAPTATTLS
                     ERDAILITYGDQVQQTGITPLRALAEFCDEVLSGVVSAIHILPFFPFSSDDGFSVIDY
                     KQVNPALGDWDDIFRMGKRFRLMFDAVINHISAQSAWFQGFLQDQPAYRDYFIVVEGQ
                     PDLSQVVRPRALPLLTSVSTSSGEKSVWTTFSTDQIDLNYQNPQVLLEIVDTLLFYIE
                     KGAEFIRLDAIAYLWKEIGTSCIHLPQTHRVVQLFHSILDIMAPQVQIITETNVPHDE
                     NISYFGDGENEAQMVYNFALPPLVLHTMQTGDARAISRWAAGLKLPSNQVTFFNFLAS
                     HDGIGVTPAKGLISPEEINMLVERALAHGGLVSYRSNPDGSQSPYELNINYFDALSDP
                     KGGELLQKQVKRFIAAHAIMFSLVGVPGIYFHSIFGSRGWLEGVAITGRNRTINRQKL
                     DLTELKAGLSDPGSTRSMVYEGLAGLLKARAAHPAFHPYGQQRILDLGARIFCILRTS
                     PDGRSRVVCLQNVSDQVVQVGLPLEGLAFDPTSLPIDIITGKRVELNSAGRLSLQPYQ
                     VLWMADI"
     gene            complement(24821..25693)
                     /locus_tag="AC16CB_10390"
     CDS             complement(24821..25693)
                     /locus_tag="AC16CB_10390"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR3342919.1"
                     /translation="MIDPKFIQLTPNTIAMLSKIGLIILLTLLGQWGIKLIFGFLEKK
                     SEKALAGTTRLSRLLTILRLARSTTIALVFLLAGMMILQALDINITPILASAGVVGLA
                     LSLGTQTLVKDIIGGILILVEDQFEVGDSVQVGEVSGAVESITLRTTSLRDLQGRLHI
                     IPNGDIRTLTNSTTEWSRAVVELNLAYDTDIKNVVRILENALEQAQNDPDVREELLET
                     PQAVGWVGMNDWAVQVRLISKTKPGSQLAVANKFRQYALDALNSAGIDMALPRQALTV
                     QDRESVARRTKGDA"
BASE COUNT         6489 a         6892 c         6336 g         6091 t
ORIGIN      
        1 agcgagccct tatatcagat accagcatac cctgccactc tccttgatga atctcacgca
       61 aacgcgcatc cgttcggatc gttaatccag tcatttccgc caggggtcgc gccgtatcta
      121 tggcgcgctg taaatcgctg gaatatattg cctggagatt agctcccgat agtttctcag
      181 ccaggtcttg ggcttgctgg cgtccttttg gattgagagc cacatcgatt tgtccttgcc
      241 agcgaccttc tgtattccaa tctgtctccc catgtcggat aaaaattaga tgcgtcataa
      301 ggagtgtaac tctctatatt ctggcacatc taccataggg ggacgttccc tctcaacgat
      361 ctcttcaacc tcgaggaaaa accgttttga ctggtaacgg atcaacttca tggtcttatt
      421 gacttcctcg aggatgttcc tgccataacg ccattccact ttccggatca ctgtctgaat
      481 aatagtgaat gacattttac tcaatgactc aagcggctgg ttgtgatgca cgcgctttct
      541 cagatccact tgagcgatgg tgtctaatcc aaagttctcc aggacatcga taagcaagcc
      601 aatctctaca ccataaccag aggagaatgg caaacgttcc agggttttgc gcctccctcc
      661 atactctcca gaaagcggtt ggatcaaccc ggaaagttcg gggtagaaca ggttaagcaa
      721 agggcgggcg gtcagttcag tcaccctgcc accacctcca gcttgaatgc gattacctac
      781 tttaatgggt cggcgataaa agcctttgac aaattgcagt tttggattgg ctaataaagg
      841 tcctatcaga ccaaagacaa aatgcggatg gatattgaca atatccgtat caatccacac
      901 caggatatca ccccgagtcg tatagaggct cttccataat gcctctcctt tgccgcgccg
      961 ggcaccatat tgagggagta tctcctgatg aatatataca ggaacaccca ggtccttggc
     1021 gatctcgcgg gtctggtcgg ttgagttgga gtccatcaaa acaatttcat ccaaaatggg
     1081 gtattcgtcc atcaataccg atttgacagt ttgaatcaca tttcccacgg tctcttcttc
     1141 attcaaggct ggcaatgcca ggctgatggt tacgccttgc ttgcgcttca gcgccagcaa
     1201 ctctttcagg tttgagaatt catcggcatg ataggtgttc tctgcgaacc atttatctac
     1261 taagacagat atggctgtct ggccgattaa ttcgctcccc atatccacag gcatcgagcg
     1321 cggcgttttt acaaccatta cgccagccga acattcgttg aggatccttt ctgctacggg
     1381 gccaagaaat tcctgcttag gtgatacctg tgctgttgcg ccgagcacta ctagatcaaa
     1441 tccatgcgaa gcctctagga ttgcctgcac tggatcatct gtgtgaatct catgatactt
     1501 gacttcaggc aggttagata gtacatgaga caatcccttg aatggagctt cctggatata
     1561 atttgaagaa ggagggatga tgtgtaatgc agtaacacta gctcgggtat ttcgggagat
     1621 cgccaaaaca atccgcaatg aaagctcggc gaatggacca ccccttatag gtatgagaat
     1681 atggtgaggt ttctcaggga tcttgccacg taccagggcc acagcgcagg tacatcgctg
     1741 cattgcctcg ttcaaggaga tccccagtgc ctcgtgctgg cgaggccatt caatgagaag
     1801 tagatcgggc tcttcgtcga tgactgcttc gaacaattcc tcagaaggtg aattcgaaac
     1861 tcgcaccctg cccaagctgc ggaagcgatc ctttttcgca aattcacgca tcatcttgcg
     1921 gacctgacgg gcttgctctg ctgaagcgct aagcggctca tcaggggcag ctgctaccaa
     1981 acccagcatc actacctcgc ctagcccgct gacggctcca gccgccctga ttgcatcttt
     2041 actttcacag ccagcaataa taggtaccat agcttttcgg aatgcaggag cagtatactt
     2101 tctcattatt catcaatcca ataacatatt tctatcatac gctagtgtag gaatctgggt
     2161 agattggggt gagtattgcc ggccccattc accatataac cctacaacga tacaacttct
     2221 gtcaataaag gttcaatctg ttcaacatat aattgttgcc aggtgtagtg tttctttact
     2281 ctgacacgca aatcatatat tgggtttgag gcaaagtgat cagaaatcat tgaagccaca
     2341 acctcggggg ctgaatcagg gtcataataa agcgcattct cgcctgccaa ttccctcagt
     2401 gggggaatat ctgcgcaaaa aatgggcaag cctgtcattc cagcttccag tataggtata
     2461 ccaaagcctt cctctcggct ggggaagaag agcgcgtcgg ccaaacggta aaaatcggcg
     2521 atcacaccat ccggcaaata ttccgagcta acttcggcca ggaaatgcgc acatcccttt
     2581 aaccctagtg actcacgcag tgagagaagc cgattgaaat agtgaatgtt agctggattg
     2641 tgtggcccca aaggtccagt gaccaccaac gccgcctgag ggaattggat ccgcagggca
     2701 gccaaaacac ccaaagctaa ttcgatattc ttgcgcgggg ttatcctgac tggcagcaga
     2761 agtagtggag cgcaccccaa caaatctagt tgtgacacca gttcaacgct ccgctcctcg
     2821 agtttcaaaa agcgctcaat gtccaaacca tttgggacaa ccaccacccg ctcgagggga
     2881 atccctaata gctcagcgag ctcttgccgg cgcaacgcag aaactaccac ctgcttggca
     2941 ccggcccaat cgcggcgcag caagtcccac ggataaccat cgtgcagttc cgaacggtag
     3001 cggggcgttg tccaggcaag gtcatgatgc cagagaatca accgcggcga attgcgctcc
     3061 tggctcaatt gatacaacgc ggcggtcagg gcaagatttt tactaagtga gcaaacgttg
     3121 tgggcaatta gcacatctgt atctagggtg atctccttca atgtggagac aagctgatct
     3181 ttcaagatag ggaattgggg cggaatattg ccctgatcca aatctcgctt gatctctaat
     3241 atattcgtat ggcgcgaatc gacgaatggg atattgacga attggacacg ctcatcgaaa
     3301 acgtctcctc tccccgccag aattcgcact tcatgaccag catctgccat caacctggca
     3361 tgatgggcca tgacactctc aacacctcct acaatcggag gtgaagagta atgcagtaaa
     3421 tcaaccttca taatcctcac catcagcacc cttggcctca ttcgtggctt ggatcaccat
     3481 ctcgatgaat aatgcggcag accatccgta gataggggca gccttcggag ggttttcacc
     3541 gctaatcgga tgataatatt cgtagatatc cggcaaactt gcaatcaaat ccagcgtaca
     3601 acggcgtaac tcccgcgcta ggtcatcgta gccacagcga aataaccctt caatcagcaa
     3661 gaaattgata ttagcccagg taggcccgcg ccacatctgc cacggatcga acttcgcatc
     3721 attcaacgca acgctaggaa caggatagcg cgcccaaaat tcgtctgggt tggtgagatg
     3781 cccgacaagg cgctcattaa tctcttttgg catttttccc gtgatcaatg ggaagagatt
     3841 gaagggggtc aacactttca cgcgttttcc caaatgtgtc gcccaaaaaa cgcctgcccg
     3901 tttatcccag cgcagcttaa tcaagcgctg tgccatggca tcagcgcgct gagaccacat
     3961 cagcccttct tcctgctccc caatctccac agcgatcttc gccagcgctt cctgctgaag
     4021 gaacagatag gtattgaggt ccggcgaatc gactggcata ccatcatccc agagcgggct
     4081 gtcatctaag cctgaagaga agggatgctg gtattcacac aaaccattct gatccagatc
     4141 gtttttctcg aaccaccaat tattccattt tattatcggt ccataaattt catttaggaa
     4201 ttcacgatca tggtcatgtt catataactt ccaggctgcc cacgccacca aagggggttt
     4261 tgtgacatcg gcctccactg gaaaatccag gtgcgttaca gtgccctcgt cgtggatggc
     4321 atcggggatc attccatcct ctcgttggtg atccatcaag atacgcaatt gatcctgggc
     4381 aaggcgcgtg tcgatatagc ggtatgctaa tgcatggaag aagttgtccc attgccatga
     4441 gccaatataa tagcttttag acggcgtcat cacttcacgg gtggtgtaat aacgcgtgga
     4501 aagcagcccc acccgcatga tccaccaggc gtagtagtat tgatgctcat attcttccag
     4561 gaccggtggg acggctgcaa accattcctc ccatcgctgt cttgaagctt caagaaccgg
     4621 ttctggctca gacacccagc ggttgaatcc caggcgcggg gttacgttca gcaataaacc
     4681 tctattgcct tccgtagcat ccaaacgaag tttcactctg tggccatcac cttccacggc
     4741 gaaggccgaa ttttcaatga tgtgggcatt ggttgtatat gccatattcc tgcggatatc
     4801 ccctgtcagt cgtagaactc ccccccgccg atccacatgg gcttgatcca gtcgagcgta
     4861 gaaatccata ccgcatcttg ccggtggcaa ggacacgtac agggtctcac tgtcttgaaa
     4921 ggccattgta aaaacgccca cgggagtatc gacaatcatg cagtgcgggt aagtggttaa
     4981 tttcagcggc ataggattgc catccccatc cgtaaaagcc cattgttcaa tcaggggtgg
     5041 tcgttgtcgg tatgccgaaa gcttaccagc cagcttgagc cagcgctcag ccaagcgaac
     5101 tgagaagtga tcatcctctt gaaatactag tatgcgcgag cctcgatcgc tgaatggtat
     5161 ctggcgtaaa ttgatacggt tttttaagag ctctatggcg ttaggtttca tgatctactc
     5221 ttcaccaaaa cattccatca acattgtatt gaggcgtcgt tcaagcatat taaacgaata
     5281 atgcctttgt gctaagctgt aattgtgctc ggtcatttgc tccgctaatc tccgatcttc
     5341 taaaataccc acaacctgtt tgactgtgtt ttcagtaata aacccatcaa attcaatcac
     5401 cttaaaaccc actggcttaa tgtcaatcgc gaagatagag taattattga ccacaattgg
     5461 acgatggtaa tagatcgcct ctaggaatgc attgccaaac ccttctgtgc ttgaagggta
     5521 agtgaccaaa tctgcagcct gataagcatc ccagagggta taaatcttgc ggccgtcttc
     5581 caagcttcca cgcgaatcgc gaatgatatc tgaaacaaaa tatgccgaca ctcccatcaa
     5641 ttccgaataa taccggagtc gctgttcata ctcataaccc tcatccccgc tggcgtgaga
     5701 tatgatcagg cgcgctttta ggcctatacg ctgaattaac tctatggcat gctcaattcc
     5761 tttacgttgg acaacccgcg taggctgtaa aaagagatac tcgccggaat tcaggccaac
     5821 atctggccgg aaggtcgccg tgtaatcatc tggtggctgg ggtggatgat caaagtccat
     5881 cacatttggg atggtcatcg cagaaatacc agttcggaga ctcaattgat ttgaagctga
     5941 cgagtttatc accacgtggc ggatggatgg caggtgcggt gggaatgaca tattcaggta
     6001 atcccacacg cagttggtta aaaacctctt acgctcccag aaaaagtcgt gatggtgtgc
     6061 gatcacaggg atgcgcgttt cggaaataaa ttcagttaga gccagaccaa gcggtatatt
     6121 cagcgggatt gccagcgcgt tctcaacaat caacagacgg atcttatatt gatcaacaaa
     6181 agcattcaag cgttgcttca ggtgttcctt aagtgtttga atccgccgtg taacctcagg
     6241 tgggcgtgtc agagccgaaa acgctatcga gtaaacttct tgaatatccg gatgcttgaa
     6301 atgcgcctct gggaccaaaa aagtataatc tttttcatat tcatcactat caccaccaaa
     6361 cagataacag ttatggccca gtctcaccaa aacttccaac cacttctctg cctcaagcga
     6421 aactccatca tgcccgcgaa atcgggtaga tacaaaacca atgttatagt tttctggttt
     6481 cataatctct cctcaatggt tattggggga tcgtcgtatc caaacaacga ataaagatat
     6541 attgattgcg aaattgttgt tgatcaaatc gagaacataa tccatcattt agaacctatc
     6601 caaaattcgg aagagtcgag ttttccatcg atacttgcaa gaacagctag gatgttttct
     6661 ttgcgataac tagcataacc ggcagacccc ataatgtcat ctcgactgcc tgttcaatgt
     6721 gaaacccggc gtgcaaaatc tcgttccgaa caaatatggg tcggcaatcg atggcagccg
     6781 gaaaatgact atgtgcccaa tcatatattc tggtaatgat attcgcatga tcctttctca
     6841 acaaagcgat gacacagctc cgccccccct tttttaagac tcgccgacat tcagataaaa
     6901 cagctttaat ttcctcgtca ttaaatagtt ctagagtgaa gctcataaat atcgcatcga
     6961 atatttctgc cttgtaaggc aatttattgg catcaccatg cgttagcgca gctcgggtgg
     7021 attgacctgc acttgcgacc ttactccggg cacgtgcaac catgccccaa gatagatcca
     7081 caccaaatac cttgcccgca tctccaacag actttgctag atctaaaaca gcctgacctg
     7141 ttccaaagcc aatttcaagg atggcttcgc cgcgctgagc atcaagcatc ctgagtcctg
     7201 ccattcgaac agaacgctca ctccatgtag ttaacaggtc ataccatctg cttaaactat
     7261 cgtaatgaga ctgggtggat tggcttgacc tatggcgggt atcggcaagg ttattctggt
     7321 tgtggatcga atccctgttc aattggcgat tcatccaact cccttagcgc agatctggca
     7381 ggcgcagcaa gtcatctgct tctctatcaa ggataccata atttgatcgg tcgtttgtac
     7441 gattatgagg tggcgctcat caataccgaa atttccatgc ttaaaatggt tgtaatgatt
     7501 attccatata ctttaatatt gtaatatcta ttgcactatc tattataatc ggatataatg
     7561 aaaagcgatg caaccagagt taaattcctc cgaagtcaac agccaaaccc tagacgaacg
     7621 catctccgca ttgcataaat ctctcagccc caaacacaaa cggctggctc gcttcgtatt
     7681 ggacaataag tacttcattt cctttgcatc agccagccaa gccggcgaaa agatcggtac
     7741 gagcgcagca acggtagtgc gcttcgctca aacaatcggc tacgagggtt attccgagat
     7801 gcaggccgcg atacgagctg agttgcccag ttacatgaca gcaattgtac gcatgcaggt
     7861 tcaattgcaa gcaaaacagc catccaatga cctgctccga tccgtatatc aagccgatat
     7921 caataacatt cagcgtacag taagcaatat ttccgaagag aaacttaatg ccgcgctaga
     7981 aactttggtt aacgccaacc gtattctggt tgtgggtgca ggcctctcgt catcgtgcgc
     8041 gatgttcctg gctcattcgc taaaaattat cggtcgagat gcacgtgtta actcaaacga
     8101 aggtctttcg ctagctgctg atatcgccct cctaaatcct ggtgacctgc tgatcgccat
     8161 cgatatgtgg cggtacgtgc gctcaaccat ccaggctgtc agcaaagcta aggaaatggg
     8221 cgtcagcgtc atcgccatca cagatagcat tgtttccccg cttgcccgcc tggcagacta
     8281 tgcttttgaa gtcgtaacca gtggtatttc acacagccta tccgcaaccg ccatcatgtc
     8341 gttgctgaac gtctttatcg ctgccctttc ctataaaatt cccgaacaaa ccatgcgttc
     8401 actaaggcgc gttgacagcg cttaccgcga taacgatcta ttggtgttgg agtgagcatg
     8461 aacatattaa tctgtggggg tggaaacgcc gcccacgccc tcgcaggctt gctctcaaca
     8521 caagaagacc tgcgagtcag ggtttacctg tcatttgaag atgaagcccg ccagtggaaa
     8581 gagggcctcg cagagcagaa aagcctccat gttcacctgc cgcaggggga gctagtgggc
     8641 cgacctgtcc aggtacgctc ccaggctgct gagattgtcc ccggcgccga tctggtattg
     8701 ttagctttgc ctgccttcgc ccatgaggtc gttttacgcc agatgacgcc acatctccaa
     8761 cctgctgcct gggtaggagc cttgccagct cgtggttgtt ttgacttgtg cgccctgcaa
     8821 acattaggag aaaaagcacc cgcagtaaac atcttcggtc ttcaaacact accttgggct
     8881 tgccggatcg aacgctacgg gcgatctgtg cgtgtgttgg gaaccaaagc acaggtggat
     8941 ttggcatcct ggccagcaga gctggctccc cagattgccg ctacgctaag ccctctgcta
     9001 ggagtagatt tacagcccgt ttcgagccta ctaagcttaa ccctggcagg taccgggcag
     9061 ttgatccacc caggaattat gtatggctta ttccacaatt gggatgggca accatatgct
     9121 caagcaccac ttttctatca aggcattgac ccttataccg ctgggattct gcaatctatg
     9181 agcgacgaag tgcaagaact gcgtcacaat ttggaggcgc gcttttcgtg gttggatctt
     9241 acagccgtgc gcaatcttta tgactggctg cgtttttctt acaccagtga tattgcggac
     9301 gatacaacct tgcaatcggc atttacaagc aatcgcagct atgcaggtct gcaggcccct
     9361 atgcgatctg tggacggcgg actggtgccc gatttccacg cccgctatat ctcagaagat
     9421 gtcccctatg cactcatcgc cacgcgaggt attgctgaat tagcgggtgt tcccactcca
     9481 caaattgatc aggtaatccg ttgggctcaa gggtgtttag gacaggaatt cctggtttcg
     9541 ggtgtgctac gcggtcaaga cctgggtgct tcccgcgccc cgcagcgtta cggttatcaa
     9601 tccatcaatt cattagtgga aagcacccga atgcctgtct gaacagcaac acatttgctg
     9661 aatcgacgaa tcatgtaagg cgaataaaca tcatttctgg agaaatatat atgccaactc
     9721 ggaataaatc gacccaaaaa gacaccaaga cctcccaaaa aaggcgcgtc atggtcggcg
     9781 caattgggaa atgtgttcac aacctgggcg ttgagaattt tgccgattgg atgcaggacc
     9841 taggcaccgg ctatgtctcg gtaaaacttg gcccagcggt cccgatccgg gaggcagtta
     9901 acaaaatacg cgaagcgcgc ccggaggtgg tgggcatctc aatgcgcctc ggagacctgc
     9961 atgttgataa gttgatcagc gaattcatcg agaccgcaac tcaatacgga cttagcccac
    10021 gcgactccgg tattcgatac agctttggcg ggctgcgacc agctgcgaat ctggtgcggg
    10081 cgatgaccgg cctgcctttg gaagaagacc gcttcatccg agatgaagaa aggcactatg
    10141 acctggccca ggtatcagag gagtacaaga agaaagagca cttccagggt ttctttgagc
    10201 ttgtggcaga cgatttcgtc tccatggaag agctggaggg ctttgcacaa cggcgttcca
    10261 ctgtcaccgc tcgtaaggac gccccgtggt ctgactattt agtggagcgc atgcgacaag
    10321 tgcgtgagcg cgaaaaccgt ccgattatcc gtgcccacat tggcatcgct gccgacacca
    10381 ttgaaccaac catcaaagcg attgagaaac ttgccgatgc tgaagccttc gagattgtgt
    10441 cgctcgcgcc tgaccagaca tcacaagaat tgctcgcaaa gttcatccgc ggcgaagaag
    10501 accccagtaa atacctggct ggtcagggag gcgcccctat acgtacagtg gaggatctcc
    10561 agcgcctaaa ggcggctacc caacgtggca attaccctat gacccggata tacaccggca
    10621 cggatgagct cgtagcccta gcgaatcttt gggaggataa tcttaatatc tgcttcccgg
    10681 ctgttcctat cttcttctat aacgagttag atgggcgtgg accgatcagc atccgcgact
    10741 cattcgacga gcatttccgt actattgagc actgggccaa gattggcaag cccttggaga
    10801 tcaatgaccc ccaccaatgg ggtttgcgat atgccagcga tgacatgcaa gtcacggatc
    10861 atgtgctctg tgcggtgatc gccctcaaga agggcattaa gaattatgtg atgcagatga
    10921 tgttcgagct cccaccagaa atatctgcct tggatgatct ggcgaaaatg agagcctcat
    10981 acgagctagc tgagccactc gcccgcaact ttgatttccg catcatccgc cagacgcgct
    11041 ctggtctgcc tagctttcca cctaacctga accaggccaa aggtcatctc gctttcggca
    11101 tctacaccca gctttacatg cagcctgata ttttgcatgt cgtcacccac tctgaagctc
    11161 accacgaagc gacagcagac gacatcattg aatcctgcga aattgtgaag caagtatgct
    11221 gggatttcgc caaaggaaat gtaccaaaga tttggtctga ccgcgaaatg aagatgcgca
    11281 tcaaagagct caagcagggc gctatgtaca acttcctgca ctgcgccatc ttaggtgggt
    11341 acgatggccc catccgtcct gaaaatttct gggagtgggc aaaagaacgc cgggaagatc
    11401 gggaaaagaa ttacgaaaca atgctgctca gcctgatcga tgaggccaac tatcccaccg
    11461 gaatatgtgg tgtgatcgca ggagatacac ttgacctggc acttcaaatc ggcctcttcc
    11521 agggaccgca tatcaccgtg gtagatcgtc gctatgagat ggcgggagcc tgccgaacca
    11581 aggttgtgaa tgggatgtgc cgcaccgagg aatggaacgg catcaaggtc tttagtgaat
    11641 ttgaaagggt cgacctggtc cgcaaaacct atccttggta tttctacaaa gaagtgaccc
    11701 gcgccgatga aatgagcttc atcgcggaag acgctgaagt agaaaagata gatgaagacg
    11761 cggtgaacga attccgcaaa cagattggaa tcacaggtct agccgatgag aaagtgctgg
    11821 tggtggattt cggcagcact tacaccaagg tcggtatctt ggataccaaa aaagagacct
    11881 tcgagctgcg ttacaagccc acaaccgtgg aagacatccg tatcggacta gccaacggct
    11941 tgggagtact agaagcctgt cgagagagtg gcgattggaa gccccttgcg aagaaaatgc
    12001 gggaattcgc cgtacgattg ccatgctcga gtgccaaagg cggcctaaaa atggttacgg
    12061 tggcgctggt aaaggaagaa tcgggatttg cggctgagtt ggcagccctt accgctggcg
    12121 cgaagcttgt cgcatcctat gatgggatgt tgactgctga gcaagctcgc caaatctacg
    12181 aagtggacca accggagatc atcctcatcg cgggcggcac tgatgaaggc ggggattcat
    12241 cgactcaact gcacaatgcc cgtctcttgg ctcgtgaatc tcgttacgct aactacactg
    12301 attacggtgt gccggtcata tatgctggca accaagacat ccgcgatcgc atccgcaaca
    12361 tttttcgcta ccgcaacata caccttcgcc tgacacccaa cgtcatgccg gagatcaatg
    12421 attttcgcat cgaagtagtt aatgaagcca tccgcgaact cttccagact gtgatcattc
    12481 gcggcaaagg ttttgacgtg gtggaggagt acatggatgc cccattcatc cccaccccgc
    12541 gagccgcttt ccgtggcatc aatctgcttg cgaatggcta tggcaatgaa gaagggttgg
    12601 gcaacataat ggcactggat atcggtggtg ctactaccga tttctactca aatgtgaacg
    12661 acaacccact atacgtattt cccggagatg acgcacaacg aaaggtgaaa cgcaccatcc
    12721 ttaaaacgcc caatgtgccg cttgtcttcc ggcgggtgga aggtaaattt ggcttgagtt
    12781 ataacgctga aaatctaaaa gagctggaga gtttcaaaga tgggtctatg gcgcgcgatc
    12841 ttagccgcta cttagatcac cgctttgtca aggaatttgc acctggcgaa gatcaattca
    12901 gacgtttcat ccaccgccag aacggctccc atcaagtaga cctggatggc tacctgagtt
    12961 ggatcagcca gaatccgcac cagaatccgg aaaccccgct ggagaacgca gccacctcgt
    13021 acctggcgcg agaaattatg accgtcacga ctgggaagca cgttggtcac gtagatgaga
    13081 ccgagacata cttcttgcaa tatggggtta actatttcaa ccaaccagtg actgtgctgc
    13141 tgatcggcgg gacaatctac caaaaatgtc gcgatcgcga gtcagggtac ctggatgacc
    13201 tgatgactat cgctgaaggt gtgctctata atccacggga agatacctcg ctgcgcccga
    13261 aagccccggt gctgctggat gccaagtacc tggtaagcat cctgggtggt ctatatggac
    13321 gtgtgaatcc cgaacaggca ttgcaagtta tgaaacgcga gctccttccg ctcgatagga
    13381 tagcgcagga atcgtcacag gctgttctta aaagctgaac attgtgtgtc acagtatatc
    13441 aaaaagcagg caggtaatat atgaaaggag gcgcctgggt aaccgcaata tgaaccaaag
    13501 caaccaataa ttcaatcaat ggattctata tatcaggagg agtaaaatgt cacgtaagtt
    13561 aagttatttt atccgtgtcc tattgccgct ggcagtcatc ctgaccgcct gcgcccaacc
    13621 acccgcggca ccaccagaag agatgatcgt taaagagacg gtcgtagtcg tagagacagt
    13681 tgtggtgacc gaaaaagagg aggtggagaa agtcatcacc cccacacccg tgcccggtcg
    13741 ccagaccatc atcgtggggc tttcccaggc tcccagcgaa ggactggatc cggccgatca
    13801 ccgcagtcgc ctgtccgaaa ctgtcatccg caatatgttt gacggactcg tcactcgcga
    13861 taacaggagc ggcgtacacc tggagctggc tgaggagatg aactggctgg atgatgtcac
    13921 cctggaagtc aaactgcgcc aaggcgtgaa attccacgac ggtgtggaga tgaccgccga
    13981 cgatgtggtc ttcaccttca atagaatcat ccaggaaaac cagatcgaat accctgagcc
    14041 ccacacctcc ccgcgcaagg gcttgattgc acccctggct tcaattgaaa aggtggatga
    14101 ctacaccgtc gtcatgaact tcagcgatcc ctggccgcct gccatgcagg gcattgtcca
    14161 ccagcaaatt gtacccaagc actaccttga agaagtgggg actgaaggtt ttgtcgccaa
    14221 tcccatcggt acaggacctt tcaagttcgt ctccgcacaa cctggatttg aagaggtcgt
    14281 cctggaaagg ttcgatgatt actacggcgg agcacctgac cttgagccag ttgggccagc
    14341 ctgtgtcagt ggtgtaatat tccgtaccat accagaagcc tccacccgtg tggcggcctt
    14401 gttggctggc gaggtcgaca tcatccagtc cgtcccggct gagctgattg acaccctagc
    14461 ccagacaccg ggcatccagg tcaagactgc agctggcact cagccgaaat ggatggaaat
    14521 gaacgtcaac aagccaccct tcgatgatgt gcgcgtgcgc caagcgatga actatgccat
    14581 cgacaaagac ctgatcattc aggagattta tggtgggcgc gccgtggccc tgcccggccc
    14641 gctctctccg tacaacaact tcgtcaacaa gagcctggta ccctacccct acgacccaga
    14701 tatggccctc tcgctgcttt ctgaggcagg ctggtccgat accaacggcg atggcctgct
    14761 ggataagaac ggggaacctt tcgtattcac catcgacacc ctcgaagact ggcgctccct
    14821 ggcagaagca gtagcagaca tgtaccgtgc cattggcatc gatgccagcg tgcgtttctg
    14881 ggagtacagc gtggtcaagc cgcagcttca ggctggcgag cgcatggcct atctggacga
    14941 ctggggggac tcagcttttg acccatttgg acacttcgaa gccaagtggc atggcctcgt
    15001 ggaagacacc tcttacggcc gcggtaactt ctccggctac aacaacgagc gcgtcaacga
    15061 actgaccaag cagggcgaga tcaccattga tatcgccgct cgccaggcga tctacgatga
    15121 agcccaacaa atcgtctatg atgaagcccc agcggtcttc ctgatcctgc cggatgaggc
    15181 cgaagctgcc tccgaccggg taaccaattg ggaacccgct gcagacagcc gcatcaacct
    15241 gcatgatgtc tgcctggctc catagatcat taagttaaag ataccgtaaa cacagtgtga
    15301 ccagcccttg tggctggtca cactgcaccg ggaatcttcg cacacaccta tgcagaggag
    15361 cgcgtatcgt gagagcggtt aaaatcctcg aacgtatcct ggcaaccatc ccaatcgtgt
    15421 ttggcgttgc cattgtggta tttttcttca tccgccttac ccccggcgac ccggttgaca
    15481 tcatgatggg tcagggcggt gctgtatcag aaggagagat tgaacagctg cgcgaagagt
    15541 tccacctgga tgaaccgctg catatacagc tctggctctt tcttaaagac gccgtgcggg
    15601 ggaacctggg ttattcatac gtactcaaac gcccggtcac taccctcatc gccgaacgcc
    15661 tgcctgccac catagaactt gctgtagggg gactgatcct cagcttgctc atcgccctgc
    15721 cgatcggaat catctccgct gtgcgccaga attcgaccct ggaccgtgtg agcatggcaa
    15781 gcgccttctt gggtatctcg atgcctggct tctggttagg catcatcctt atcctcttct
    15841 tttcggtgcg cctgaactgg ctgccagtgc agggcaggat taccttcggt accaacctgc
    15901 aggaaatcac aggctatttt gtactggata gcctgctctc aggcaatcgg caggcgctga
    15961 tcagcagctt gaagcacttg atactcccct ctatcaccct cggggcgccg gttgcggcgg
    16021 tagtagcccg cgtgttgcgc tccagcatgt tggagacgct acgttcggat tatgtgaccc
    16081 tggcgcgcag taaaggtgcc acagagtgga gcgtagtgct aaagcatgcc ctgcgaaatg
    16141 ccctgatacc caccgtgacg gtagtcggct tgcaagtggg catcttgctg ggcggaaata
    16201 tggttgtgga aaccgtcttt ggttggccgg gactggggcg catggtggtg agcgccatct
    16261 ttgaccgcga ttttccgctt atccagggtt ccgtcatgat atatgctttc accttcgtga
    16321 tggctaacct gatcgtggat gtactctata cctatctcaa tccaaagatt tccttataga
    16381 cacctaactt gtattcattt caatggcaca cattaaatcg ataggaaagc taaaccgatg
    16441 gaagtagccc cccaagttcg aaccccattc tcgcaccagg tctatgtgtg gcgaatccgg
    16501 ctggcgattt ggcgacaaaa tgctgccgaa tttctatacg agttgcgcaa gcaccctctg
    16561 gcattgcttg gtggtttggt gatcgttgta tacgtggtat tagccatctt tgccgggcaa
    16621 gtaaccgttt acgacccggt ccgggggaat ctacgcttgc gcctggatcc cccggcctgg
    16681 gaggaaggcg gcagctggga gtatccgctg ggcaccgacg cccaaggccg agatttgctc
    16741 acgcgcatca tctatggcgc tcgggtttcc ttacttgtag gttttctatc ggtgggcatt
    16801 tcagtgattt tgggcatgat caccggcacc ctggctggct attaccgagg ctggttcgat
    16861 aacctgatct cgcgctttgc cgacctattg ctagcattcc catttttgat ctttgccatt
    16921 ggtatgatgg ctttcttggg gccgggtttc atcaacctga tcctggcact gaccttcaaa
    16981 gggtgggtgg agttcttccg gcttgtgcgt ggagagatgc tctctgaaaa gacccaagaa
    17041 tacgtagaag ctgcccgtgt ggctggcttg agccacctgg cgattatcgc tcgcgagatt
    17101 atccccaata ttatccagtc ggtgtttgtg ctgggcactt tgagaatagg ctatatgatc
    17161 atcatggaag ccagtctgag ctttttaggg cttggcatcc ctccctccat cccggcttgg
    17221 ggttcgatgg tctcggcagg gcgagattat atgctagtag cgtggtgggt ttccaccttg
    17281 cccggtattg ccatcttgat cctggtacta gcgatcaact tgttcggcga gggattgcgt
    17341 gacatcttag atccccgcct gaagattgaa ggataatcca cagcacgtgt gaggcccgca
    17401 tgccaaaaca ggctttactt gagctacaaa atcttacgac agtatttcct acccggcgcg
    17461 gacaggtgcg cgcagtcgag agtctcaatc ttgaattatt cgagggcgag attcttggga
    17521 tcgtaggcga atctggctat ggcaaaagca cagtgttact ctccatcttg cgccttatac
    17581 gccagccagg ttatattgcg tccggcaaga ttcgctaccg gggtcgcgat ctgcgagaac
    17641 tctcgacacc agcgatgcgc tccgtgcgtg gcaaggaaat cgccatgatt ttccaggatc
    17701 cacagtccac cctaaatccg gcttttacgg tgggcgagca gattcgcgaa gccttgcgcc
    17761 tgcaccatat cgtcccgggt aacccatatc cctggcctct ggattacgtc gttcgcacgc
    17821 gtgagaaaaa gcgcgtgctg gaggtgatgg aggaagtggg catcccctcg cccatggatc
    17881 gctacgcatc tttcccacac cagttctccg gtgggatgca acagcgcgcc ctaatagcca
    17941 gcgctggtat gcgggccagc actgctgctg gcagatgagc caactacagc gctggatgta
    18001 accatccagg ctcaaatcat cgacctaatg cgccgtatca accaggagcg tggtaccgct
    18061 ctcatcctgg tgacgcacga cctgggttta gcagccgagt tttgcagccg catcgcagtg
    18121 atgtatgctg gtcgcattgt ggaacaggga accgtggacc aaatggtaga aaatccacaa
    18181 catccctaca cccagggtct gctgaattgt cggccgcgca tatccatgcg cgaactgcgt
    18241 gtggaaccaa tccctggcaa tgttcccgat ttagccgagc tgcctgaagg ttgtgccttt
    18301 gccccgcgtt gtgcacatgt ccaacctgcc tgccaggcag gccctattcc tatggtgagc
    18361 attaaggcag accgcctcag ccgatgtctg ctccacattg gttaccatcg ggaagatgcc
    18421 tggaagtggg atcatacggt gaatagccca tgaaggaagc acaatcagag agtacaccca
    18481 cacccttatt tgaagggaag ggaatttata tgcacttcca gatccgccct agtttgctag
    18541 ctcgggcatt agcgggagta aatgaaaagg ttgtccgcgc ggtagacggt gtcgacctgg
    18601 cgatatggcc cggtgaaacc ctcgggctgg ttggcgaaag cggctgtggg aaaaccaccc
    18661 tcggtcgtgt gctcacgcgc ctgtatgaac ccactcgagg ccagatctat tatcgaggtc
    18721 aacctgtaaa aggtgatact atcctcgagc aacaggatgg cagcaaccaa actcgccgcg
    18781 tgaattatca tcgccttgcc caaattattt tccagaaccc ctactcgtca ctgaacccgc
    18841 gcaaaacagt gcgcgagatc atcggcacac ccttacaaca acgtggcgtg cgtgatcctc
    18901 tgctacgtga agaagaaacc cggcaacttt tacaacgagt aggtttgagc aagcgacaca
    18961 tcgaccgcta cccacaccag ttctcaggag ggcagcgcca gcggattggc atcgcccgct
    19021 ccttagccat gcggccaaaa tttatcgttg ccgatgagcc ggtttcatcc ctagatgttt
    19081 ccattcaagc tcaagtgatc aacctattgg aagagcttca ggcagaatat cacctcacct
    19141 acctgtttat tgctcatgac ctgagcgtga tctactacat cagcgacagg gtagcagtga
    19201 tgtacctagg acacatcgtc gaactgggta aaactgacta tttgtttgaa tcgcctctgc
    19261 atccctacac gcaggcgtta ctggcggcaa tcccgcgggt cagcaaggct gcccgcaagg
    19321 agcgcattct tctcccagga ggtgtgccca gcccgattga ccccccatcc gggtgtccct
    19381 tccatccacg ctgctctgcc aaagttggac ctatttgtga acaagaatac ccgccattct
    19441 ttcaggtaaa taaccagcaa gtcgcatgct ggctgtatca agaccacagc atagcagacc
    19501 aatcaagcac taagtaaaac tgcggagaca tccatttgat gcgccagctc tatctttcag
    19561 gggaccattt cgcaatgggt aagcagcatg gtcaccaggt gaccgacctc aaaccccata
    19621 ttttatctgc gatccaacac cgactagata aacttgccaa ggtcgagctt aacacgggac
    19681 accttgagga gcaaatgctg gaatcttggc acgcccaagc tgaaccactg ctggaaatga
    19741 tgcagggcat cgcagaagcg ctaagtttgg attggcgggt cttcttccgc tataccattg
    19801 ccacattttt aatggatatg gctcgccaac cgcaagttgg cgatcaggga tgcaccacct
    19861 gggcagttct atcagaggcc acccgtgatg gcataccgtt attggtaaaa aaccgcgatt
    19921 actggcctga ccaccaggaa ctgcagtgcc tggcgcatac ccgtccagat aaaggttacg
    19981 tatacttgaa catgaccagt gcaggtagcc ccggcgtctt tagctctggc attaacgaag
    20041 ccggattggc ggtctccgat acgcatgtat catcactgga tattggccca ggattgcctc
    20101 gctattacct gatgatggag attttagagc atcacgacac agtgcactcg gcggttgatt
    20161 accttcgctc ggtcaatcat gcggggaatg gcacgctgtt attactcgat gccaacgggg
    20221 aggcagcagt atttgaatgc ggtcaaattt tttcgaaatc aatcccagcc accaacggat
    20281 ttgttgtcag tgccaaccac tatgtaaccc cagccctgcg ccagaaatgg atgtttagaa
    20341 ccacagacag aaagttgata ggcagctcct tgcatcgcta ctcccgcgtg aagagggcgt
    20401 tattaaaagc gaaaggcagt attgacctca actgggcgat caacttaatg cgacaacacg
    20461 gtaccccatt gaacgcgatt tgccgccacc ccgagctgaa cccacaatcg gtgacgattt
    20521 ccagtgcaat ctacctgcca acaaaaaaac agctttatct ggttaatggt attccctgcc
    20581 aaaatgaata ccaggtatac ccggttattc cagacgttca ggacgaaaaa caggcctacg
    20641 ctctctgaac gccctttttt acaggtctgt tcgaatttat tccccccaat tgcacaattc
    20701 ctgaatttac tcggcctaga tcatcgctat gccctaaggg gcttaaattg ttagatccgt
    20761 gtaggctcca tgactgtttg gcgcaggaat gagtgcagtg gaccgcttcg ccgcttgaca
    20821 atgaccaccg taaccgatgc cctgcgagcc acttgttcag atatattccc catgcgcaac
    20881 ttttttagca agggctctaa tgtcgcccca atgacgatga gatcagattg gggcgtgtct
    20941 tcatcacctg ctgcttcgct cagtatagtc tcgatgatat tgctgccttc aaccacgcgc
    21001 ttttcgatat tctcataggc tatacctgac atcgattctc gaaaagcctg ttgcgctcgc
    21061 acgatagccc cattgcgcgc gccgaatggg atgatgtgca acaacgaaac caacgccggc
    21121 ccgccttcac cagacatagc catgtcgaca gccagcttga cagccaggcg actgttcggc
    21181 cctccagcaa cagggacaag gatcttctta atgctgcggc gctggcgata gcgcacgatg
    21241 gcaatatccg tgggcgggtt atcaataatc ggatcaatga ccgagccata gattctcccc
    21301 gcagtatggg tgtaccccgg ccatcctaaa acaagcaggt ccgatgcgct ctcatcaatt
    21361 gttttgtgga cggcctcggc tacactgcgc cccaaacgaa tcaccgtatg cacaggcaca
    21421 tcaaatggtt ttgcacggtg aatcacggtt tctaggtaag accgaccttc tttcaaaaat
    21481 agacgcccct ctccgagggt gaactggcgt ggcacccgta ctacatgcaa ggccagcacc
    21541 tcgccgttgt tgacttttgc taaaattgcc ccgatttgac ccaggatcat cgcctgctcc
    21601 tgtgtagcga cgggtaccac gatggagtaa tcccgggtga ccagaacttc ctccatcaat
    21661 atctctttgg gtttctccat ggcttcaatt ttggaaaaat acgtgtaata aatcagcaat
    21721 ccaaagatga cccaggcgat ggctgtatac caggacacgg gcgcataact gaacaaagat
    21781 aatgccagga ttgcattgga gaataatgcc agaataggaa tgaaagggtg ccaggggact
    21841 ttataacctc gctctaaatc gggcattttg tgtcgcagtg tcatgacagc cagattcacc
    21901 tggaggaaaa gcatcaagaa catgatatcc gccgcggctg ccacctcttc gattggcaat
    21961 atccaagcga tcaaaagcat gagtgcgccc gaagcgatga ccgccaagta aggcgtatgc
    22021 cggagcgggt gaatgcgggc aaagatagat ggcaagttat gatcgcgtcc cattgcaaag
    22081 gagactcgtg aagaagaata ggtggtagcg ttgagcgccg aagtggtcga aacgagacca
    22141 cttaccaaca tgatgactgc ccctacccca agtggaaaaa tctgcgccgc aacaccaaca
    22201 actgcaatct ctccctgttc ccccaggtac tcatacgcct tcatgccctg gggcggggtt
    22261 accgccccaa tggcagtaac tccaacaaac acataaatga ccacgactaa aattatggct
    22321 aaaaagatcg cccggggcac gtttttccga gggtttatta cctcttcacc agtttgcgca
    22381 ataatctcat atccctcgaa agcgatgaat gttaacccca ttgcaatgat tacccctagc
    22441 atgccatttg gcatgaaacc gctcgtaaag cgcgtgtgcc aggcatcggt gcgcagcatg
    22501 gcaagcacac caaacaaaac aaataccccc aagatcagga tctttgtcat ggtgatgata
    22561 ttaccgattg tcccggtctc agaagcgccc cgatagttaa gatatgtaaa ggcggcgatg
    22621 atgagggtca taaatatcaa ggtcatctgt tcgacctcga gattaaaggt gggaacacct
    22681 gccagaaccc agagttcaga gaaaaatctg ccaaatccta gagcatataa actgccagcc
    22741 actgcgtgag cgaaccaact catccagcct gctaaaaatc cctgggcacc gcctaggccc
    22801 tctttcaccc agagatatcc gccgcccgct tccggaaatg ccgagcccaa ttcagcataa
    22861 accatgcctg tcaatattgt caccagcccg tttagaacaa acgccaggat cagcgcagga
    22921 cccgctacgc cagccgcgat gccggtgagc acaaaaatac ctgcgccaat catgccgccg
    22981 actccgatca tggtgatcgt gaacaaaccg aggctgcgac ttaaagtaac tttctcaggg
    23041 atggattgtg tggtgttggg ggcaatcgtg ctcatggatc gcttgtccct ttcaagttta
    23101 gatatccgcc atccacaaaa cctggtaagg ctgcagtgaa agcctgccag ctgaattcag
    23161 ctccaccctt tttcctgtga tgatgtcaat gggtagggag gttgggtcaa atgccaaacc
    23221 ttcaaggggc aatccaacct ggacgacttg gtcggataca ttctgcaggc agacgacacg
    23281 tgaccttcca tctggtgatg tgcgcaagat gcaaaagatc ctggcgccga gatccaaaat
    23341 ccgttgctgt ccataagggt ggaaggccgg atgggcagca cgtgctttaa gcaagccagc
    23401 caggccctca taaaccatgg agcgggtgga accagggtct gaaagaccag cttttaactc
    23461 cgtcaaatca agtttctggc ggttgatcgt ccggtttcta ccggttatcg ccacgccctc
    23521 cagccagcca cgcgagccaa aaatggagtg aaaataaatt ccgggcacac ccaccaggga
    23581 aaacatgatc gcatgggcag cgatgaagcg cttaacctgt ttctgcaaaa gttctccacc
    23641 cttagggtcc gatagtgcgt caaagtaatt gatattgagc tcgtacgggc tttggctgcc
    23701 atccgggttc gagcgatatg ataccagccc tccatgggct agcgcccgct caaccagcat
    23761 atttatttcc tcaggagaga tcagcccttt agccggtgta acgccaatgc catcatgcga
    23821 agccaagaaa ttgaaaaaag tgacttgatt tgatggcagc ttcaatccag ccgcccaacg
    23881 cgagatcgcc cgagcgtccc cggtttgcat ggtgtgcaag accaatggtg gcaaagcgaa
    23941 attatagacc atctgggctt cgttctcccc atcgccaaaa taagaaatgt tctcgtcatg
    24001 gggtacattc gtctcggtga tgatctgcac ctgaggtgcc atgatatcca gtatgctgtg
    24061 aaacaactgt accacccgat gggtttgagg caggtgaatg caagaggtac caatctcttt
    24121 ccagagatat gcaattgcat ccagacgaat gaattccgca cctttttcta tgtaaaagag
    24181 cagggtatcc acgatttcaa gcaagacctg tgggttctgg taattcaggt cgatctggtc
    24241 agtgctgaag gtggtccaca cggatttctc gccagacgaa gtgctgaccg acgttagcaa
    24301 gggcagcgcc cgcggcctga cgacttgtga taggtccggc tgaccttcaa caactatgaa
    24361 ataatcccta taagcaggtt ggtcctgtaa aaacccttga aaccacgcac tttgagctga
    24421 gatatggttg atcaccgcat caaacatcaa acggaatcgt tttcccatcc tgaatatgtc
    24481 atcccaatct cccagggcgg gattgacctg tttataatcg atcacggaaa aaccgtcatc
    24541 cgaagaaaac ggaaagaacg gcaagatatg gatggcactg acaaccccgg atagaacttc
    24601 atcacaaaac tcagccagcg ctcttagggg cgtgatgcct gtttgctgca cctgatcacc
    24661 ataggtgatt agaattgcgt cgcgctcgct tagagtggtt gccgtagggg cagcaatcct
    24721 agaccgatac cggtcgatca acctcaccaa tctatcgtaa gtggcgcgac cctcttgatc
    24781 tccatagaga tatacaaaat gagcgagaat cttgtccatc tcaggcatcg cccttcgttc
    24841 gcctcgctac agattcccga tcttgcaccg ttaaggcttg ccgagggagt gccatatcaa
    24901 ttccagcaga gttgagcgca tccagagcat attggcgaaa cttgtttgcc actgctagtt
    24961 ggctgccggg cttggtcttg gatatcaatc gcacttgtac agcccaatca ttcatcccta
    25021 cccaacccac tgcctgaggc gtctcaagta attcctcacg gacatctggg tcattttgag
    25081 cttgctcaag agcattctcc aggattcgaa ccacattctt tatgtcggta tcataggcta
    25141 ggttgagctc tacgactgcc ctcgaccatt ccgtggtgct gtttgtaagc gtgcgtatgt
    25201 cgccattagg aatgatatgc aaacggcctt gcaagtcgcg caggctggtg gtgcgcaatg
    25261 tgatgctttc taccgcacca gatacctcgc ctacctgtac tgaatccccc acttcaaact
    25321 ggtcctcgac caaaatcaat atccccccaa taatatcctt aaccagggtt tgtgtaccga
    25381 gtgagagcgc tagcccgacg acgcctgcac tggcgagtat tggcgtgatg ttgatatcca
    25441 atgcttgcag gatcatcatc cctgccaata ggaaaaccaa tgcaatggta gtgcttcgtg
    25501 caaggcgcaa gatcgtcaat aagcgcgaca agcgggttgt accagcaagc gctttttcac
    25561 tctttttttc cagaaaccca aaaatcaact ttatccccca ttgacccagc agcgtaagca
    25621 aaatgatcaa gccgatctta gacaacatag cgatcgtatt cggggttaat tgaataaatt
    25681 tagggtctat catcctaatc accttcttct agctacatta tttgcaatca tttcaatcgc
    25741 caatgccgcc gaccatgaga aatccagcga gccacaacca ctcccatctt gcggatcgta
    25801 atactcgc
//
LOCUS       DBNGSX010000345         3569 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_445089, whole genome shotgun sequence.
ACCESSION   DBNGSX010000345 DBNGSX010000000
VERSION     DBNGSX010000345.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_445089"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="AC16CB_10395"
     CDS             complement(<1..283)
                     /locus_tag="AC16CB_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342920.1"
                     /translation="MKLRFDPAEQERFWDAIRRIPGYPVGDQIRLKTMLIESGALFRL
                     PSVLAALTEKSQGEVLVVMDPTPMRRGSDSLKPLVLDVLRNAGWQPNTLV"
     gene            complement(291..1091)
                     /locus_tag="AC16CB_10400"
     CDS             complement(291..1091)
                     /locus_tag="AC16CB_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HIR3342921.1"
                     /translation="MRRPLNEIQCFLLDMDGTFYLDDQLLQGALHFIDVITRLEHKYI
                     FLTNNSSRHRGYYAEKITRLGLVVQESQIFTSGEATALYLKSASPGARIFVVGTTFLE
                     EEFRQHGLTFDEEDPEYAILGFDTTLTYHKMWKLCDFVRAGLPYIATHPDLNCPTESG
                     FMPDIGAMIAFVQASTGRLPDKVIGKPNRAIIDELAKKIQLPVEHFAMIGDRLYTDIA
                     LGETTGITTILVLSGETQLSDLDTSPHKPDYIFNHLGEIAEYLTSNHH"
     gene            complement(1088..2617)
                     /locus_tag="AC16CB_10405"
     CDS             complement(1088..2617)
                     /locus_tag="AC16CB_10405"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF012589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIR3342922.1"
                     /translation="MDYVIGCDVGSQAVKAVLLSTEGQILAEAGASYDISFPRPAWAE
                     QDSNSWIQGVVQAISKLVREAGVRREQIRAIGLDAQVDGFVPIDAAGQPLHAGIIWMD
                     RRAVEQTESIRKQYDSSRLFELSGLNLDPYHVAPKICWFAETHPKLYEKSRYFMLPGS
                     YVAYKLSGEYGVDYSNASSTLLMDVRKKEWSTELCNYFQIAIDKLTPIYPANSVIGSL
                     LPEMAEQMGLDAQTKVILGCGDEHAASLGAGVIIPGLVCDIAGTAEPIGAASQQPLFD
                     ETGLVETHCHADPDLWFLENPGFVSGGNYRWLRDQFARDQELEATKLGIDIYDLLNRE
                     AENVPPGSDGLILLPCLSGAMTPTWNAYARGVFFGFTLAHQRGHFIRAVLEASAYALR
                     DITDRMQAMGLALTQIRAVGGGARSPLWRQIKADVTGLPIALLQTVETTALGAGLLAL
                     AGMGYTRDLTEAVEVAVRVVETRDPNPQTGEIYEAYYQLYRAAYFAMLPIFEQASKIS
                     P"
     gene            complement(2641..3342)
                     /locus_tag="AC16CB_10410"
     CDS             complement(2641..3342)
                     /locus_tag="AC16CB_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/alpha barrel domain-containing protein"
                     /protein_id="HIR3342923.1"
                     /translation="MKPELIVMLTHQDMTVKNANELFEECKDLPVTHWGFKDVGLPHE
                     QMKELVRRMKVEGKVTYLEVVSLSEEDGLVGAKLAVEAGFDILMGTVYFDSIHQFLKD
                     KPIKYYPFPGHVHSHPSILDGTIEEIVNHAISLQSTGVQGLDLLAYRYVGDARKLLLD
                     VVKATNIPLVCAGSIATFQRIAEVWEANSWGFTIGSAFFDMKFVPGGSFKENIEAVTD
                     WLAKTDEADVPGYLR"
     gene            complement(3382..>3569)
                     /locus_tag="AC16CB_10415"
     CDS             complement(3382..>3569)
                     /locus_tag="AC16CB_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3342924.1"
                     /translation="ILTSDTTRPVASQIPSLIRPHGTLWGEVCALATAASLPVAFLIF
                     ALQRQLVRGLSFGAVKG"
BASE COUNT          884 a          913 c          883 g          889 t
ORIGIN      
        1 gcaccagagt attgggttgc cagccggcgt ttcgcaaaac atccagtacc aggggtttca
       61 ggctgtcaga tccacgcctc atcggggttg gatccatcac caccaacact tctccctggg
      121 atttttccgt taacgcggct aaaacagatg gcaatctgaa aagagcccct gattcaatca
      181 acattgtttt caggcggatt tggtcgccaa ccggataacc tgggatacgc cggatggcgt
      241 cccagaatct ttcttgttct gctggatcga atctcaattt catcgttttc ttaatggtga
      301 ttgctggtta gatattctgc aatttcgcca aggtggttga aaatataatc tggcttgtgg
      361 ggagatgtgt ctagatcaga tagttgagtc tccccgctga gcaccaggat ggtagtgatg
      421 ccagttgtct ccccgagagc gatatctgta tacaggcgat ctccaatcat tgcaaagtgc
      481 tcaacgggta actgaatttt tttggcaagc tcatcgatga tcgcccggtt tggcttgcct
      541 atgactttat ctggcaggcg gccagtggaa gcttgtacaa atgcaatcat cgccccaata
      601 tcgggcataa aaccgctttc ggtggggcaa ttgaggtcag ggtgggtggc tatgtacggc
      661 aaacccgcgc gcacgaagtc acacagtttc cacatcttat gataggtcag cgtggtgtcg
      721 aaacccagga tagcatactc aggatcttcc tcgtcgaagg ttaaaccatg ctggcggaac
      781 tcttcttcca gaaatgtagt ccccacaacg aagattctcg caccaggcga tgcagacttt
      841 aaatacagcg cagtggcctc gccagatgta aaaatctggc tctcttgaac aaccagtcca
      901 aggcgcgtaa ttttttcagc ataatagcca cgatgcctgg aagagttatt agtcaggaat
      961 atatatttat gctcaagcct ggtgattaca tcgataaaat gtaaagcacc ctggagcagc
     1021 tgatcatcca ggtaaaacgt tccatccata tcaaggagga aacactggat ttcattgagt
     1081 ggacgtctca tgggcttatt tttgaggctt gctcgaaaat tggcagcata gcgaaatagg
     1141 cagcgcggta tagctggtaa tatgcctcat aaatttcgcc tgtctgggga ttggggtcgc
     1201 gggtttcgac cacgcgcacg gcaacttcca cagcttcggt caaatcgcgt gtatacccca
     1261 tgccagccag agcgagcagg ccagcgccca atgcggtggt ttctactgtt tggagcaaag
     1321 caattgggag acccgttaca tcggctttta tttgccgcca tagtgggctg cgcgctccgc
     1381 caccaacagc tcgaatttga gttaatgcta aacccatcgc ctgcatccgg tcggtgatat
     1441 cccgcagggc ataagcggat gcctctaata ctgcccggat aaaatggcct cgctggtggg
     1501 cgagggtaaa tccgaaaaac acacctcggg cgtatgcatt ccaggtgggc gtcatcgccc
     1561 cagaaaggca agggagtaag atcaatccat cgctgcccgg gggtacgttt tccgcttccc
     1621 ggttgagtag atcgtagata tctataccga gcttggtcgc ctctaattcc tggtcgcgag
     1681 cgaattggtc tcgtaaccag cgataatttc ccccggatac aaagcccggg ttttccaaaa
     1741 accacaggtc tggatcagca tgacaatgcg tctctaccag gccggtttca tcgaagagcg
     1801 gctgctggct tgctgcacca attggttcgg ctgtgccggc gatatcgcat accaaccctg
     1861 gaataatcac gcctgctccc aagctggcgg cgtgctcgtc accgcaaccc aaaatcactt
     1921 tggtttgggc gtctaaaccc atctgctcgg ccatctccgg aagaaggctt cctattacgc
     1981 tattggcagg gtagatcggc gtgagtttat caatggcgat ttggaagtaa ttgcataact
     2041 cagtggacca ctcttttttg cgcacatcca ttaacagtgt cgaagatgca ttggaatagt
     2101 ccacaccgta ttcccctgat agcttgtagg cgacgtaact gccgggtagc atgaaatatc
     2161 gtgatttttc gtacagtttg gggtgggttt cggcaaacca acatattttt ggagctacat
     2221 gataaggatc aaggttcagg ccagaaagct cgaataatcg ggatgaatca tactgtttcc
     2281 ggatggattc cgtttgctct accgcgcgac gatccatcca gatgatccct gcatgcagtg
     2341 gttgaccagc ggcatcaatg ggtacgaagc cgtccacctg ggcatccaga ccaatggcgc
     2401 gaatttgctc tcttcggact ccagcctctc taacaagctt tgagatcgcc tgtacgacac
     2461 cctgtatcca agagttagaa tcttgctctg cccaggctgg gcggggaaag gatatgtcgt
     2521 aactggctcc cgcttcggca aggatctgcc cctccgtaga gagcagaaca gcctttacag
     2581 cttgagagcc gacatcacag ccaattacat aatccataag tgccttgggg ttggaaagcg
     2641 tcaacgcaaa taaccaggga catccgcttc atccgttttt gccaaccaat ccgttaccgc
     2701 ttcaatattc tccttaaatg agccacccgg aacgaatttc atatcgaaga atgcactacc
     2761 tatggtaaat ccccatgaat ttgcttccca aacttcggca atacgctgaa aagtggcaat
     2821 actccctgca cagacgaggg gtatgtttgt ggctttaact acatctagca ataacttgcg
     2881 ggcgtcaccc acgtaacggt aggctaataa gtcgagcccc tggactccag tggattgcaa
     2941 agagatggcg tggttgacaa tttcttcgat cgtgccgtcc aagatcgaag gatgactgtg
     3001 gacgtgacct ggaaatggat aatacttaat cggtttatcc ttcaaaaatt ggtggatgct
     3061 gtcaaagtaa acagttccca tcaggatgtc gaagccagct tcaactgcga gcttcgcacc
     3121 aactaatccg tcttcctctg agaggctgac cacctcaagg taagtgactt tcccttctac
     3181 tttcatccga cgaacgagct ctttcatctg ctcgtgaggc agcccaacat ccttaaagcc
     3241 ccaatgggtt actggcaagt ctttgcactc ttcgaagagc tcatttgcat ttttaacggt
     3301 catatcctga tgcgtgagca tgacaattaa ttctggtttc atcgtcaatc cctccaaata
     3361 atctacccag ctatggtgaa tctagccttt aactgcgcca aaagataacc cacgcaccaa
     3421 ctgtctttga agggcgaaga tcaagaatgc cacgggcagg gaggctgcgg ttgccagggc
     3481 gcaaacctcc ccccaaagcg tgccatgtgg gcgtatcaat gatgggatct gagaggcaac
     3541 cggtcgcgtg gtatcgctag tcagtatca
//
LOCUS       DBNGSX010000346         4833 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1137028, whole genome shotgun sequence.
ACCESSION   DBNGSX010000346 DBNGSX010000000
VERSION     DBNGSX010000346.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4833)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1137028"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1652
                     /locus_tag="AC16CB_10420"
     CDS             <1..1652
                     /locus_tag="AC16CB_10420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3342925.1"
                     /translation="LLSPTCPSTILLGKEPENMPDLSLYENYDVIVIGAGHNGLVTSA
                     YLAKAGLRVLVLEQSHVLGGASSTEEIFPGYKFSVFSYVVSLLRPEIIRELDLPKHGL
                     AILPLESTLTPLPDGDYLYRDADHYRNMREIARFSPRDADAYEDYSHTMYFMAKAVKY
                     ILGIHPPNPASLHPNDLAGLYRLARHFLNLPGEQIHTLARLMTMSSADFLERWFESEP
                     LKGTLSASGIIGTFLGPRSPGTAYVLLHHYMGEIDGNFRSWGFAKGGTGGISQAIAGA
                     ARSLGAEIRLNSPVSEVIVKGGRAVGVALRDGKEIAAKVVVSSLDPNQTFLKLVDPNV
                     LPNDLVSSVRKYVYRGSSGKVNLALDAAPQLARMPDGGAALRGAISISPSVDYIERAY
                     DQAKYGDFSQRPYIDIIIPSMIDPNMAPPGKHVMSCFVQYASYHLQEGSWEQRREAFG
                     DAVVDTLSEYIPNLKDIIVYRQVITPYDIEQSIGLTEGNIFQGELSLSQLFFLRPAPG
                     YANYRTPVKGYYQCGSATHPGGGISGAPGRLAALRILKDLR"
     gene            1649..3292
                     /locus_tag="AC16CB_10425"
     CDS             1649..3292
                     /locus_tag="AC16CB_10425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3342926.1"
                     /translation="MMSTVSKGEEFDAVIIGAGHNGLVTAAYLAKAGLRVVVLERREV
                     LGGAAATEEIFSGYQINTGTCDTSLLLPKIISDLDLETNGVEFIHPPAAIFAPQLDGR
                     PLTIWRDPQRTREEIAAFSKKDADNFPGYLRLVSRLGEILRDTMTLTPPAIPEITFGS
                     VLPWLPLALKVKRLGDHDLAELLRVVPMPVKDFLDEWFENPLLKAALGVSGVIGSMQG
                     PRSPGTSLSLLYQANGAGSSGFPNTRLVRGGTGVLSQALADFARKHGADIHLGEPVEA
                     INLDRGQITGVTLTSGERISSRIVLSNANPRHTFFDLVGAPNLPLHFVREMRDFKYRG
                     STTRINLVLSGFPSFNTASSDENLTGRIVICPNLDYLERAYDSAKYGSTPTQPYLDIV
                     IPTLSDPSLAPEGAHIMCIDVRYTPYNLSDSTWDKQKERLGDQVIKLLSAYAPDIKDL
                     VTHRQILTPLDLERHYGLPEGNIYHGQMGLDQLFFMRPSAGSHGANTPIPGLYVCGSG
                     AHPGGGLSGAPGYNAARQMLKEMRKEISSRRKGKKPQTA"
     gene            3362..4462
                     /locus_tag="AC16CB_10430"
     CDS             3362..4462
                     /locus_tag="AC16CB_10430"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3342927.1"
                     /translation="MDLEPDEDFGCSKIPTRPNDISLSNAVQSGGKRILLVKTLLSSA
                     CERDCYYCPFRAGRDFRRVNFKPEELAQTFMSFYQAGIAQGIFLSSGITGKGYRTQDR
                     LIDAAEILRSKLGYRGYLHLKIMPGSERAQVERTMQLADRVSVNLEAPNQQRLQVLAP
                     GKQFLQELIRPLQWVEEIRQTRPAQLGWNGRWPSSVTQFVAGGAGESDLELLSTTEHL
                     YRQLRLQRTYFMAFRPIPDTPMENQPAVSPLRERRLYQASFLIRDYQFSYEEFLFDQR
                     GNLPVETDPKTAWAEIHLSGRPMEINLASHQELLRIPGVGPKGAGAILAARRLRKFRS
                     LSELNKIGINTHRIAPFIIIDGKLPTRQGKFW"
     gene            4541..>4833
                     /locus_tag="AC16CB_10435"
     CDS             4541..>4833
                     /locus_tag="AC16CB_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HIR3342928.1"
                     /translation="MNPIRIGIDIGGTFTDFVIFRPDTGRIETFKLPSTPHHPAEAVL
                     TGLRRVTGSENNLVIEIIHGSTVATNALLERNGARTALITTRGFGDVIQIGRQ"
BASE COUNT         1190 a         1184 c         1189 g         1270 t
ORIGIN      
        1 agctgttatc accgacctgc ccttctacaa tcctgttagg aaaagagcct gagaacatgc
       61 cggatttaag cctatacgaa aactacgatg taattgtgat cggtgccggt cacaatgggc
      121 tggtaacgag tgcttatttg gcgaaagccg ggctcagggt tttggtgctg gagcaaagcc
      181 atgtacttgg tggagcgagc tccactgagg aaatttttcc tggttacaaa ttctctgtat
      241 tctcctatgt agtcagttta ttgaggccag aaattatccg cgaactagat cttccaaagc
      301 acggcctcgc cattcttcca ttggagagca cccttacacc cttgccggat ggagattatc
      361 tatatcgaga cgcagatcat tatcgcaaca tgcgggagat cgctcgtttt tcccccagag
      421 atgctgatgc ctatgaggat tattcccaca ctatgtactt tatggcaaaa gcggtaaagt
      481 atatcctggg gatccatcca cctaatcctg ccagcttgca tcccaatgat cttgctgggc
      541 tttaccggct cgctcgccac ttcctcaacc tgccaggtga acaaatccac acactcgccc
      601 gactgatgac catgagttcg gcagacttct tggaacgctg gtttgagtcg gagccactca
      661 agggaaccct ctctgccagc ggcatcattg gcacattttt agggccgcgc tcaccaggta
      721 cagcttacgt gctcctccat cattatatgg gtgagatcga tggaaacttt cgctcctggg
      781 gttttgcgaa aggtggcacg ggtggtattt cgcaagctat tgccggcgct gcgcgttcct
      841 taggagcgga gatacgccta aattctcccg taagtgaggt gatcgtcaaa ggcgggcggg
      901 ctgttggtgt agctctacgg gatggcaaag aaatagccgc caaggtagtt gtatctagtc
      961 tggatcccaa ccaaacgttc ttaaaactag tcgatccaaa tgtcttgccc aatgacttgg
     1021 tctcctcggt gcgcaaatat gtatataggg gatcttccgg caaggttaat ctagcccttg
     1081 atgctgcccc acaattagcc cgtatgccag atgggggcgc tgcattgcgc ggggcgattt
     1141 cgatcagccc cagcgtagat tatattgagc gcgcctatga tcaggcaaag tatggggatt
     1201 tctcgcaacg cccctatata gatatcataa tcccatccat gatagatcca aatatggcgc
     1261 caccgggcaa gcatgtcatg tcttgctttg tgcaatatgc atcctatcac ttacaagaag
     1321 gctcgtggga gcagcgccga gaagcatttg gtgatgcagt agtcgatacg ctatccgaat
     1381 atattccgaa tctaaaggac atcatagtat accgacaggt gataacccct tatgatattg
     1441 agcagtctat tggtttaacc gagggaaata tcttccaagg ggagctcagt ctctcgcagc
     1501 tcttcttttt acgaccagct ccaggatatg caaattatcg cactccagtc aaaggctact
     1561 accaatgtgg ttctgctacc catccaggag gcgggatctc gggtgcaccg ggtcgcctag
     1621 cagcattaag aatattaaag gatctacgat gatgagcact gtttcaaagg gtgaagaatt
     1681 tgatgctgta atcatcggtg cagggcataa tggactggtg acagcggctt atttagccaa
     1741 agcaggactg agggttgttg tactagaacg aagggaagtg ttgggtggtg cagctgcaac
     1801 ggaggaaatt ttctccggtt atcaaattaa tacagggact tgcgatacaa gcctattgtt
     1861 gcccaagatc atctcagatc tcgatctaga aaccaatggt gttgagttca ttcacccccc
     1921 tgcagcaata tttgcacctc aattagatgg tcgtccactg acgatctggc gagatccaca
     1981 acggactaga gaagagatcg ctgctttttc gaaaaaggat gcggataatt tccctggtta
     2041 ccttcgattg gtttctcgat tgggggaaat tctgcgggat acaatgacac tcacccctcc
     2101 agcgatccca gaaataacat ttggatcggt tctcccctgg ttacctttgg cattgaaagt
     2161 taagcgcttg ggtgatcatg atctcgcaga actattgaga gttgtgccca tgccagtgaa
     2221 ggattttctg gatgagtggt ttgaaaaccc tcttttaaaa gccgcattgg gagtatctgg
     2281 ggtgattgga tcaatgcaag gaccacgttc gccggggaca tcattatcac ttctttacca
     2341 ggctaatggt gcagggagta gtggattccc caatactcgc ttggtgcgag gcggaaccgg
     2401 ggtattatcg caagcgttgg cagattttgc gcgtaaacac ggggctgata tccacttagg
     2461 cgagcctgtt gaggcgatca acttggatag aggtcagatc acaggcgtaa ctttgacgtc
     2521 tggtgagcgc atctccagtc gcatcgtttt atcaaatgct aatccgagac atactttctt
     2581 tgatcttgta ggcgcgccta atctgcctct ccatttcgtt cgagaaatgc gtgatttcaa
     2641 gtatcggggc agcaccaccc gtattaattt ggttttaagt ggcttcccca gttttaatac
     2701 ggcctctagc gacgaaaatc tcactggtcg aattgtgatt tgccccaatc ttgattattt
     2761 agagagagca tatgactcgg ctaagtatgg cagtacgcct actcagccat atctggatat
     2821 cgtcattccc acattgagcg atccttcatt agctcctgag ggtgcgcata ttatgtgcat
     2881 tgatgttcgt tacactcctt ataacctgag cgacagtacc tgggacaagc aaaaggagcg
     2941 tttgggcgat caagtgatca agctgctctc agcctacgct ccagatatca aagatctggt
     3001 cacccaccgt caaattctga caccgcttga tttggaacga cactatggct taccagaagg
     3061 taatatttac catgggcaaa tggggttgga tcaactcttt ttcatgcgac cctctgccgg
     3121 ctcccatgga gccaacaccc ctattccagg tctgtatgtc tgcggatcag gtgctcatcc
     3181 aggtggaggc ctttcaggtg ctcctggcta taacgccgcg cgccagatgt tgaaagaaat
     3241 gcgcaaagaa atttcttccc gtcgcaaggg taaaaagcct caaaccgctt gacatccaga
     3301 acaaatattc tataattagc gtgtggatgc tgttggacgt ctgaagcttc tatctgccca
     3361 aatggatctg gagcccgacg aggatttcgg atgctccaag attcccacgc gacctaatga
     3421 catctcgctg agtaatgctg tgcaatcggg gggaaaaaga attctattgg taaagaccct
     3481 cttatcatct gcctgtgagc gagattgtta ttactgtcct tttcgagccg gtcgtgattt
     3541 tcgccgggta aattttaaac ctgaggagtt ggcacagaca tttatgtctt tttaccaggc
     3601 cggcatagcc caggggattt tcctcagctc ggggataacg ggcaaaggtt atcgtacgca
     3661 agatcggcta atcgatgcag ctgaaattct acgttctaaa ctcggatacc gaggttatct
     3721 ccacttaaag atcatgcctg gatctgaacg tgcgcaagta gagcgcacta tgcagctggc
     3781 tgatcgcgtc tctgtcaatc tggaagcgcc caaccagcag cgcttacaag tgctagctcc
     3841 tggtaaacag ttccttcaag aactgatccg gcccctccaa tgggttgagg aaattcggca
     3901 aacgcgacct gctcagcttg gttggaatgg gcggtggcca tcttcggtaa cacagttcgt
     3961 cgctgggggt gcaggtgagt ccgatctgga attactgtcc accactgaac atctataccg
     4021 acagctccgt ttgcagagga catatttcat ggctttccga cctatcccgg acaccccaat
     4081 ggaaaaccag ccggcagttt cgccactgcg ggagagacgt ctctaccaag catctttcct
     4141 gattcgcgat tatcagttca gttatgagga atttctgttt gatcaacgcg gaaatcttcc
     4201 cgtggaaact gatcctaaaa ctgcctgggc agaaatacat ttatctgggc gacccatgga
     4261 gataaacctt gctagtcacc aagaacttct gcggataccc ggggtaggtc caaaaggcgc
     4321 tggcgctatt ctcgcagccc gccgcctgcg taaatttcga tcactaagcg aattaaataa
     4381 gattggtatt aatacgcatc ggatagctcc ctttattatc atagatggca aattaccaac
     4441 gcgccaggga aaattttggt aaaaatccca tcttctgacc tcacgcttgc tctcttgcag
     4501 atttctctag cttgttctat acttttccaa aggttggcta atgaacccga tccgcatcgg
     4561 catcgacatt ggcgggacat tcaccgattt tgtcatcttt cgtcctgata ctggacgcat
     4621 cgagacgttt aaattacctt ctacacctca ccaccctgcc gaggcggtgc taacgggttt
     4681 aaggcgcgtg actggctcgg agaataatct tgttatcgag ataattcatg gctcgactgt
     4741 tgctacaaac gcacttctgg agcgcaatgg agcacgcacc gcccttatca cgacacgcgg
     4801 ctttggtgat gtaatccaaa ttggtcgcca gga
//
LOCUS       DBNGSX010000347         3445 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1068791, whole genome shotgun sequence.
ACCESSION   DBNGSX010000347 DBNGSX010000000
VERSION     DBNGSX010000347.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1068791"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="AC16CB_10440"
     CDS             complement(<1..1147)
                     /locus_tag="AC16CB_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HIR3342929.1"
                     /translation="MLSTTNQIRSLGFTTRQLHAGQEPDPSTGARAVPIYQTTSYVFN
                     DTDHAARLFALEEMGNIYTRIMNPTNDVFEQRLADLEGGVGALSASSGHAAQSMAILT
                     LCGAGDHIVSASTLYGGTYNQFCYTFPRLGIDVTFVDPSDPENFRRAIRPNTKLLWGE
                     TLGNPRINIFPIEEVAAIGQEFGIPLAIDNTFATPYLCRPFEWGANIVVHSATKFIGG
                     HGTSIGGIIVDGGYFEWAKNERFSNFNIPDDSYHGLVYADLGPAAFITKARVQILRDI
                     GACQSPFNSWLFLQGLETLSLRMERHVSNAQSVAEFLQDHPHVSWVSYPGLPSHPDYH
                     AAQKYLPKGPGAILGFGVRGGLEAGRRFIDRLQLFSHLANVGDAKSLA"
     gene            complement(1570..1905)
                     /locus_tag="AC16CB_10445"
     CDS             complement(1570..1905)
                     /locus_tag="AC16CB_10445"
                     /inference="COORDINATES: protein motif:HMM:NF014132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HIR3342930.1"
                     /translation="MAEYTVEQLVRNHEKAFKPEAAEGVDAIIQYHLTGEEGGDWIIN
                     IHEGKCKVAQGVAENPKMTLTAEAQDFKDVLTGKANGMQYFMQGRLMLAGDLNLAMKL
                     TTFFKMSSN"
     gene            complement(2007..3212)
                     /locus_tag="AC16CB_10450"
     CDS             complement(2007..3212)
                     /locus_tag="AC16CB_10450"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HIR3342931.1"
                     /translation="MMINRSDPNREAVIVAAVRTAVGRAKKGSLATVRPDEMAALVIR
                     DLLERAPALDPAQVDDLILGCAFPEGEQGLNLSRLVGFRAGLPDSVPAETINRFCSSG
                     LQAIAHAAFAIMAGQMEVAIAGGTESMSMVPMTGFKFSPSPYLAENAPQAYTNMGLTA
                     ENVAAKYHISREDQDLFALRSHQRAVAAVEAGLFDAEIIPVDVDIVESNGNGKPKRRS
                     FTFSREEGPRSDTSLEALAKLRPVFKEGGTVTAGNASQMSDAAAGVIIMSRAKAEALG
                     LTPLVRFVAFAVGGVPPEIMGIGPVVAVPKALKLAGLSLKDIGLIELNEAFAAQAVTV
                     IRELGLDEEITNVNGGAIALGHPLGATGAKLTTQIISEMKRRDVQFGMVTMCIGGGMG
                     AAGIFENLN"
BASE COUNT          821 a          949 c          860 g          815 t
ORIGIN      
        1 tcgccaaact ttttgcatcg cctacattgg ccaaatggct gaaaagttgc aatcggtcga
       61 taaacctgcg ccctgcctct aatccccctc gcaccccaaa accaaggatc gcccctggtc
      121 ccttcggtaa gtatttctgg gcggcatggt aatctggatg agaagggaga ccagggtacg
      181 acacccagct aacatgggga tgatcctgca ggaattcagc caccgactgg gcattgctca
      241 catggcgttc catccgtagg ctgagggttt ccaagccttg caagaacagc caagagttga
      301 agggcgactg gcaggcacca atatcgcgta aaatctggac acgcgccttg gtaatgaacg
      361 ccgccgggcc tagatcagca tacacaagcc catgatagct atcatcgggt atattgaaat
      421 ttgaaaatct ttcattcttt gcccattcaa aatatccacc atctacaata atgccgccaa
      481 tggatgtgcc atgaccgccg ataaatttgg tcgcggaatg gacgacgata ttggctcccc
      541 attcaaaagg ccggcacagg tatggcgtag caaaggtgtt atcaatcgcc agcggtatgc
      601 cgaattcctg ccctatggca gctacttcct cgatgggaaa gatgttgatg cggggattac
      661 ccagcgtctc gccccacagc aatttggtgt taggacggat ggcgcggcga aaattttcgg
      721 ggtcggatgg atctacaaag gtcacgtcaa tgccgaggcg gggaaaggtg tagcaaaact
      781 ggttataggt gccgccgtaa agtgtcgagg cagacacaat gtgatcccca gcaccgcaca
      841 gtgtcaaaat cgccatgctt tgcgcagcat gacccgaact tgcagacagt gcacccacac
      901 ccccttccag gtctgccaag cgctgctcaa acacgtcgtt cgtggggttc atgatgcgag
      961 tgtaaatgtt tcccatctcc tccaaagcga agaggcgggc agcatggtcg gtgtcgttga
     1021 aaacatatga ggtggtctga tagatcggca cagctctcgc tcctgtggag ggatcgggct
     1081 cttgtccagc gtgtagctgg cgggtggtaa agccaagtga acggatttgg ttggtagtgg
     1141 atagcatcgg ttgtttctcc ttttttattt tcggtataaa aaaagcggcg gacagcttgg
     1201 cgcaatctct aaaagagttt tgcgagaagc tgtccgccgc cttggcaaac ctggcttaag
     1261 cgtcaatcag gccaattgac cctcacgctc cttcgaggcg gtggttgcag ctcccgcctc
     1321 gtgcacatca acatcgacat aaatagcaac ataaaaaact tcctgttatc ctgtgctaga
     1381 aattgtgcca attctaagaa ataaatcgta ttttgtcaat gatcaggaac aaattcacgc
     1441 actggctgga aaggtacctc gccagtgcgt gaatactctc atctataacc ccaggtgcct
     1501 tgctgtcttc aggtcactgg ggattaacag tcggtcaaaa gcgttaagca gctctggctc
     1561 ttgtgttggc taattcgatg acattttgaa gaaggtagtc agcttcatcg ccagattcaa
     1621 gtcacccgcc agcatcagac gaccttgcat gaagtactgc atgccattag ccttgccggt
     1681 gagcacatcc ttgaaatcct gtgcttcagc agtgagggtc atcttgggat tttcagccac
     1741 accttgggcg accttgcatt taccctcatg gatattgatg atccaatcgc caccttcttc
     1801 accggtaaga tggtactgga tgatagcatc caccccttca gcagcttctg gtttgaaggc
     1861 tttctcgtgg ttgcggacca gttgttcaac agtatattca gccataaata atcgctccta
     1921 tagaaaaaga gaatgttggg ttaaagcttg taattcatga taagccatct gtcaatatcg
     1981 gcttctatgc gtaagccgga ctgattctaa ttgagattct caaatatccc agccgcgccc
     2041 atgccgccac cgatacacat agtcaccata ccgaactgca cgtcgcgacg cttcatttca
     2101 gagatgattt gcgtggttag cttggcgcct gtggcgccca gtggatgccc caaagcgatg
     2161 gcgccgccat tgacattagt aatctcctcg tccaatccga gctcgcgaat cacggtcact
     2221 gcttgggcag caaaagcctc gttcaattca atgagaccga tatccttgag tgataagccc
     2281 gccagcttga gcgccttcgg cacagccacg accgggccaa tacccatgat ctccggcggc
     2341 acacctccca cagcaaaagc gacgaagcgc accaatggcg taagtcctaa ggcttcggct
     2401 ttggcgcgcg acataatgat caccccagca gcggcatcag acatttgcga cgcattcccc
     2461 gccgtgacag tcccgccttc cttgaacaca ggtctgagct tcgcaagagc ttccaggctg
     2521 gtatcgctgc gtggtccttc ctcccgagag aaggtaaacg atctacgctt gggcttgccg
     2581 tttccgtttg attcgacgat atccacatca acagggataa tttccgcgtc aaatagccca
     2641 gcttctactg ctgccacagc gcgttggtgg cttcgcaaag caaagagatc ttgatcctcc
     2701 ctgctgatgt gatatttcgc agcaacattt tccgcggtca atcccatatt ggtgtaagct
     2761 tggggagcgt tctccgccaa ataaggggag ggcgagaatt tgaaaccggt cattggcacc
     2821 atgctcatcg attctgtacc cccggcgatg gcgacctcca tctgcccagc catgatggcg
     2881 aacgccgcgt gggcgatggc ttgcagtcca gacgagcaaa aacggttgat cgtctcagct
     2941 ggcactgaat ctggcaatcc agcccgaaat cccaccaagc gcgagaggtt caaaccctgc
     3001 tccccttctg gaaaggcaca accgaggatc aagtcgtcaa cttgggccgg atccagcgca
     3061 ggggcgcgct ccaggagatc acggatcacc agcgcagcca tctcatctgg gcgaacggtc
     3121 gcaaggctgc cttttttcgc cctgccaacg gcggtgcgca ccgcggcgac gatcacagcc
     3181 tcgcggtttg ggtcacttcg gttaatcatc atgttctctc ctaattgcac acggataaac
     3241 atagccactc aatggagctg attcgtgtat attcttcata tcgatccacc taatttctta
     3301 gaggctttcc cgtttgcaac aagttccaca tgcgttcttg ggtcttcttc tctcctgcca
     3361 gcgagagaaa tgcctctcgc tccaggtcca gaatatattg ctcatcgacc caagttgggc
     3421 tgctcagttc gccgccagtc atcac
//
LOCUS       DBNGSX010000348         3112 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_930995, whole genome shotgun sequence.
ACCESSION   DBNGSX010000348 DBNGSX010000000
VERSION     DBNGSX010000348.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_930995"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="AC16CB_10455"
     CDS             <1..926
                     /locus_tag="AC16CB_10455"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3342932.1"
                     /translation="SAVVYDQGRSTIVRALTGRDDFRIRIVTKQEEMEELITQSPDVI
                     IGLVIPADFDQRAGSSEAISLEGYTNHWADPEKINQRLAFFEEQLGLATWGSVQIDVD
                     GRVLYPSVGPKGQPYVFLLLLSITILTIGVTIVSVSFVEEKEAHTFQALLVSPAGFGQ
                     IVTAKALVGIIFCFIASGVVILANAYMVVHWEIALLGILLGAILAAGIGLLMGGISDN
                     PSTTGMWAALLIIALLFLTFMQEFSRDNWPHLVRLALSWVPGPALIKLLGAAMAWEIP
                     AGLLWTNAIALLSACVLVYGLVFWRIRQFDR"
     gene            932..2509
                     /locus_tag="AC16CB_10460"
     CDS             932..2509
                     /locus_tag="AC16CB_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3342933.1"
                     /translation="MKFRHISALIALFVVACSQGVISPTSTPTVNHPTLAPAFLPEFA
                     PPGTFQPAACRFILPEDTQEGEDVECGYLFVPESRDAQAQPGGRVIRLAVAIFHPPGG
                     PTRPDPLMYLSGGPGVSALELIRYQFDLLSEPAFSTGRDLILFDQRGVGLSRPALDCP
                     GFDQLALELVDREVDGRQVSDEEIETHLLNSLISCRDELEKIADLSEYNTAASAADVN
                     DLQAALGYPEINLWGGSYGTRLALEVMRRYPQGPRSVVLDAVYPPDVDLYAQAPDNFS
                     RALERMFESCANNPVCSAAFPDVRQVLFETVAQLNTKPAMSEITNPFTGEKYQSWMDG
                     NTILALTFQLLYDSQLRYLIPQYIYDASQGNFTAFDQVRGALIGLRSRTSRGMMLSVQ
                     CQEELAFSSLQDFQSRLARHPELEDTYHNSLMGEIVYSICQEWNVGAADASSKQPVSS
                     AVPTLLMSGEFDPVTPPAWGQQAAKTLTQAYSYEYPGIGHGASVMAGCPQEMMVAFLA
                     NPSVAPNSACIEAMRKP"
     gene            complement(2618..>3112)
                     /locus_tag="AC16CB_10465"
     CDS             complement(2618..>3112)
                     /locus_tag="AC16CB_10465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012797.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR3342934.1"
                     /translation="FSWGVALVAPQDPGNLGTILRTIDAVGASGLLLLDSNLDPYHPS
                     AVRASMGALFWYPLVQASFAEFASWVKERGYTVYGTSAHASTDYRRLTVAGSSDQPSR
                     ADQPQAQTYSKPMILLLGSERQGLTEEQVAVCHHLLRLPMLGHATSLNLAVAAGVMLY
                     AMLE"
BASE COUNT          683 a          872 c          872 g          685 t
ORIGIN      
        1 cttcggcggt tgtttatgac cagggtcgct caacaattgt acgggcgctc accggtcggg
       61 atgatttccg aatacggatt gtcacaaaac aagaggagat ggaggaattg atcacgcaat
      121 ctcctgatgt gatcattggc ttggtcattc cagcagattt tgatcagagg gccggcagca
      181 gtgaagcaat cagcctggaa gggtacacaa accattgggc tgatccagaa aagataaacc
      241 aaagactggc ctttttcgag gagcaacttg gcctggcaac ctgggggagt gtgcagatcg
      301 atgtggacgg gcgagtcctg tatccatcgg ttgggccaaa aggccaaccg tacgtattcc
      361 tcctccttct aagtatcacg atcctgacga ttggtgtcac catcgtctcc gtctcgtttg
      421 tcgaggagaa agaggcccac accttccagg ctctgctggt gtctccagcc ggttttggac
      481 agatagtaac tgcgaaagcc ttggttggga tcattttctg cttcattgct tccggggtgg
      541 tcatcttggc gaacgcctac atggttgtcc attgggaaat agcgttgctg ggtattctgc
      601 ttggtgcgat cctggcagca gggatcggct tgctcatggg tggaatctct gataacccgt
      661 caacgaccgg gatgtgggcc gccttactga tcattgccct gctctttttg accttcatgc
      721 aggaattcag cagagacaac tggccccact tggtcagatt agcactctcc tgggttcctg
      781 gccccgccct gatcaagcta ttgggggcag cgatggcatg ggagatcccc gccggattgc
      841 tctggacgaa tgcaattgcc ttgctgtctg catgtgtttt ggtatacggg ctggtgttct
      901 ggcgcatccg ccaattcgac cggtaaatgc catgaagttc aggcatatct ctgcgcttat
      961 cgcgctattc gtggtcgcct gcagccaggg ggtaattagc ccgacatcta cccccacagt
     1021 caaccacccc acgctagcgc ccgccttctt acctgaattc gctccaccgg gcaccttcca
     1081 gcctgccgcc tgccgcttca tcctgccgga ggacacacag gaaggagaag acgtcgaatg
     1141 cggctacctg tttgtgccag aaagccgcga cgcgcaagca caaccaggcg ggcgcgttat
     1201 ccgcctggct gtagcgattt tccacccacc gggagggcca acccggcccg acccgctcat
     1261 gtatctctcg ggcggacccg gagtcagcgc cctcgagctg atccgctacc aatttgatct
     1321 actttctgag ccagcattct caacaggcag agacctgatt ttgttcgacc agcgaggggt
     1381 aggtttatcc cgtccagccc ttgactgccc tggttttgac caactggcct tggaactggt
     1441 tgacagggag gtggacggtc gccaggttag cgatgaagaa attgagacgc atttgttaaa
     1501 cagcctcatc tcgtgccggg atgaactaga gaaaatcgcc gacctttcgg aatacaacac
     1561 ggcagccagc gctgccgatg tgaacgacct gcaggcagcg ttaggttacc ctgaaatcaa
     1621 cttatgggga ggttcctacg gcacccgcct ggcattggaa gtgatgcgcc gttacccgca
     1681 agggccgcgc agcgtggtgc tggatgcagt ttacccgccc gatgtagatc tttacgcgca
     1741 agccccagac aacttttccc gtgccctgga gcgcatgttc gaaagctgcg ctaacaaccc
     1801 ggtatgcagt gcagcttttc cagatgtgcg ccaagtgctg tttgagacag tcgcccaatt
     1861 gaacaccaaa cctgcgatga gtgaaattac aaacccattc accggcgaga agtaccagag
     1921 ctggatggat gggaacacca tcctggcgct gacattccag ttgctctatg acagccaact
     1981 gcgctatcta attccgcagt atatctatga tgccagccag ggaaacttca ccgccttcga
     2041 ccaggtgcgc ggggcattga ttggtctgag gagccgcacc agccggggga tgatgttatc
     2101 ggtacaatgc caggaggagc ttgccttcag ctcgcttcaa gacttccaaa gcaggcttgc
     2161 ccgacaccct gagttggaag atacgtatca caattcgcta atgggtgaaa tcgtctatag
     2221 catctgccag gaatggaatg tcggggcggc agacgccagc agcaaacaac cagtttctag
     2281 cgctgttccc accctattga tgagcggtga atttgatcct gtcaccccgc ctgcgtgggg
     2341 acagcaggca gctaagacgc ttacacaggc gtatagctat gaatatccgg gaatcgggca
     2401 cggcgccagc gttatggctg gctgcccgca agagatgatg gttgccttcc tcgccaatcc
     2461 ttcggtcgcc cccaatagcg cctgcatcga agcaatgcgc aagccataat ttaaacacct
     2521 gctttaattt cgggcaggtc accaatcgct tgttctgaag ttacgaagtt gggaaggtaa
     2581 aagggggtta gtaaatcaat ttcgagatag tgtagagcta ctcgagcatt gcatacagca
     2641 tcaccccggc tgccacagcc agattcaggg atgtagcgtg accgagcatc ggcaagcgca
     2701 agaggtgatg gcagaccgcc acctgctcct cggtcagtcc ctggcgttcg ctgccaagca
     2761 gcaggatcat gggctttgaa taagtctgcg cctgtggctg gtctgctctc gagggttgat
     2821 cggacgaacc agccacggta agccgacggt agtctgtcga ggcatgggct gaggtgccgt
     2881 acactgtgta gcctcgctcc ttgacccaag acgcaaactc tgcgaaagat gcctggacca
     2941 gcgggtacca gaacaacgcc cccatgctgg cgcgcaccgc gctgggatgg tagggatcca
     3001 agttgctatc cagcaggagc agcccgctgg cacccaccgc atcgatcgtg cgcaatattg
     3061 tccccaggtt gcctgggtct tgcggggcaa ccagcgccac cccccaggaa aa
//
LOCUS       DBNGSX010000349         3049 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1761455, whole genome shotgun sequence.
ACCESSION   DBNGSX010000349 DBNGSX010000000
VERSION     DBNGSX010000349.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1761455"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="AC16CB_10470"
     CDS             <1..437
                     /locus_tag="AC16CB_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012966992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase C-terminal domain-containing protein"
                     /protein_id="HIR3342935.1"
                     /translation="DSPSLEYREWGQLPYLQKAARMAYQQAGIRSPIGVVDFAEVDDL
                     YAYKELQSLEALGLCGVGEAGSITEEGLTEADGELPVNVSGGSLGMGNLLDATGLSRA
                     LEVVLQLRGEAGPRQLENAEIGLAQSWRGVPTTSAAVAVFSN"
     gene            450..1616
                     /locus_tag="AC16CB_10475"
     CDS             450..1616
                     /locus_tag="AC16CB_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014512167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HIR3342936.1"
                     /translation="MGMRKVAVIGAGMTKFVRRAQETAKELSWEAARMALETSELTLD
                     DVDAVCMGTAPDAFDSVHMNGEYLSDGAGAWRKPYMRSYVGGGTGVFAPIQGWYHVAS
                     GLFDVVLVVAEEKMSSFYPHAQAAFLTIFDHTTERPLKPNLLWIFALEMNRYMQTYGI
                     SKDDIARVSVQHKRNAADHPCALLGDADITVQDVLNSEVLAWPVQRLDVSPVSDGAVA
                     IILAAEHIANRVTDKPVWIEGVGWSLDTAYWANRDLAYPRYVESAARMAYDMAGIREP
                     RKQINIVEPYDPFDYKALHHLEGLQLADRGQAPQLYIDGIAARDGNMPVCPSGGLLGV
                     GNPIAAAGLMKIAELYWQLRGEAGNRQIPGSPRRGLAQAWGDLMQVGTVVIMGR"
     gene            1636..2151
                     /locus_tag="AC16CB_10480"
     CDS             1636..2151
                     /locus_tag="AC16CB_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010878466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR3342937.1"
                     /translation="MTAKITNFPGTPLGEEDFAEGKVLFNYDQMRGDFAWDTGIAIGR
                     YLAALKEGIILGARCATCRKTVVPPRTVCEWCFRPMQEYVPLQDSGIVNTFSLCYVTW
                     DVQRIKEPEIPAVIEIDGASPLHGIMHKIGGVDPQKVHIGMRVKAVWRPPREREGAIT
                     DIMYFKPIEEV"
     gene            2153..2620
                     /locus_tag="AC16CB_10485"
     CDS             2153..2620
                     /locus_tag="AC16CB_10485"
                     /inference="COORDINATES: protein motif:HMM:NF013920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR3342938.1"
                     /translation="MSILERDVNAPASWNGNLPVTSRYTFGLAGERFFRAIKDEGRLY
                     GTHCPNCNRTYVPAASFCERCLSELTEWIDVGLVGEVHTFTLLHENYDGSPRETPEVV
                     AFIRLEDGGLVHRIDEIQPDEIEIGLHVEAVFKPKVERNGSILDILYFRPANL"
     gene            2799..>3049
                     /locus_tag="AC16CB_10490"
     CDS             2799..>3049
                     /locus_tag="AC16CB_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342939.1"
                     /translation="MTLILPGILGVLTPLLYGVARARYGILNYGPVAADLWSRPWYLL
                     AIIAYLIFAYAAISRIMASRRYFAIYEGGVAHNLGRKMT"
BASE COUNT          717 a          760 c          825 g          747 t
ORIGIN      
        1 atgattcacc ctctctggaa taccgcgagt ggggacagct cccataccta caaaaagcag
       61 cacgaatggc ctaccagcaa gcgggcattc gcagcccaat tggagtcgta gattttgccg
      121 aggtagacga cctctatgcc tataaagagc tgcaatctct tgaggctttg ggtttatgcg
      181 gtgtaggtga ggcgggatcg atcaccgagg aaggcttgac cgaagcggat ggcgaattgc
      241 ctgtgaatgt atccggtggg agtttgggta tgggaaatct gctcgatgca accggcctct
      301 cgcgagccct ggaggtcgtc ttacaattgc gaggtgaagc tggtcctcgg caactcgaga
      361 acgccgaaat cggcctcgcc cagtcctggc gtggcgttcc caccaccagc gctgcggtcg
      421 ctgtttttag caactgaaag gaggtaatca tggggatgcg taaagttgcc gtgattggcg
      481 ccggcatgac caaatttgta cgccgtgccc aggagaccgc caaagaactt tcctgggaag
      541 cggcgcgtat ggcgctcgaa actagtgaat taaccttaga cgatgttgac gcggtttgca
      601 tgggaactgc ccccgacgca tttgacagcg tgcacatgaa tggggaatat ctcagtgatg
      661 gtgctggcgc ttggcgaaag ccatatatgc gctcctacgt aggcggtgga acaggggtat
      721 ttgcacccat ccagggttgg taccacgttg catccgggtt atttgatgtc gttctggtcg
      781 tggcagaaga gaaaatgtca agcttttacc cacacgccca agccgctttc ttaaccatct
      841 ttgaccacac tactgaacgc ccgctcaaac ccaacctgct gtggattttt gctctcgaga
      901 tgaaccggta tatgcaaacc tatggcatct cgaaagacga tatcgctcgg gtttccgttc
      961 aacacaagcg caacgccgct gatcatccat gtgcgctgct aggtgatgcc gacatcactg
     1021 tccaggacgt actcaactca gaagtgctag cctggccagt ccaacgccta gacgtcagcc
     1081 ctgtaagtga tggagctgtg gcgattatat tggcggctga gcatatcgcc aatcgagtca
     1141 cggataagcc agtctggatc gaaggcgtag gttggtccct ggataccgct tactgggcaa
     1201 accgcgatct tgcctatcca cgctatgtcg aatctgctgc gcgtatggct tatgatatgg
     1261 caggcatccg cgagccgcgt aaacaaatta atatcgttga gccttatgat ccattcgact
     1321 acaaagccct ccaccactta gaaggcttgc agctagcaga tcgagggcag gcacctcagt
     1381 tatatatcga cggtatcgct gcccgcgatg ggaatatgcc cgtttgccca tcgggtggtc
     1441 tcttaggcgt tggcaatcca atcgctgcgg ctggcctaat gaagattgct gaactctact
     1501 ggcagttacg tggtgaggct ggcaatcgcc agattcctgg atcgccccgc agaggccttg
     1561 ctcaagcctg gggtgacctg atgcaggttg gcacggtcgt gatcatggga cgttaaatgt
     1621 tagcggagaa tgaatatgac tgcgaaaata actaattttc ctggtacacc cctgggtgaa
     1681 gaagatttcg ccgagggaaa ggtgttgttc aattacgatc agatgcgcgg cgacttcgcc
     1741 tgggatactg gtatcgctat cggtcgctat ctggcagctc tgaaagaagg gattattttg
     1801 ggagcgcgct gcgctacctg tcggaaaacc gtcgttccac cccgcacggt ttgcgaatgg
     1861 tgttttcggc ctatgcaaga atatgtaccc cttcaggatt cgggcattgt caacactttt
     1921 tcgctttgtt acgtcacttg ggatgtgcaa agaataaaag agcctgaaat acccgccgtt
     1981 atagagattg atggcgcatc ccctctgcat ggcataatgc ataaaattgg aggcgtcgac
     2041 cctcaaaaag ttcatattgg catgcgtgtg aaggcagtct ggcgtccccc tcgtgagcgc
     2101 gagggagcaa tcaccgacat aatgtacttc aaaccaattg aggaggtgta atatgagcat
     2161 cctcgaacgt gatgttaacg ctcccgcttc ctggaatggg aatctaccgg ttaccagtcg
     2221 ttacaccttt ggccttgcag gtgagcgatt tttccgagcg atcaaagatg agggacgttt
     2281 gtatggcacg cattgcccaa actgcaatcg cacatatgtg ccagcagctt ccttctgtga
     2341 gcgctgtcta agtgagctca ccgaatggat cgatgtaggt ctcgtgggcg aggttcacac
     2401 cttcacgctc ctccatgaaa actacgacgg gagcccccga gagaccccag aggttgtcgc
     2461 cttcatccgc ctggaagatg gcggattggt acaccgaata gatgagattc aacctgatga
     2521 gattgagatt ggcctgcacg tagaggcagt attcaaacct aaggttgagc ggaatggttc
     2581 tatattggat attctatatt tcaggccagc caatttataa gcaaaacatt gattgacgcg
     2641 gctggcatga tcattgcatc cataggtgcg atgaactttc agaagttcat cgcacccaaa
     2701 tttaggttgc tatataagct gtgccttcta tcgctaaaaa aaacctcgga cgattgatca
     2761 aaatatttcg cttccgcccc atgggctgga gtgaggctgt gacactgatc ttgccgggca
     2821 tcctgggagt gctcacacct ttgttatatg gcgttgcacg cgctcgctac ggtatattaa
     2881 actacggccc ggtggcagcc gatctatgga gtagaccctg gtatcttctt gcgatcatcg
     2941 cttatctaat atttgcctat gctgccatct cacgcataat ggcatcccgc cgctatttcg
     3001 ccatttatga aggcggagtg gctcataatt tagggagaaa aatgacctt
//
LOCUS       DBNGSX010000350         6293 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_237430, whole genome shotgun sequence.
ACCESSION   DBNGSX010000350 DBNGSX010000000
VERSION     DBNGSX010000350.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6293)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6293)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6293
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_237430"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1454)
                     /locus_tag="AC16CB_10495"
     CDS             complement(<1..1454)
                     /locus_tag="AC16CB_10495"
                     /inference="COORDINATES: protein motif:HMM:NF024832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HIR3342940.1"
                     /translation="MSEKVIDRAINAGMSSSSRERSSERNIVAIAKDGGITFAGKMFL
                     SAVRLVTAVIVARLLGADQYGMYNLALSTANIAIGLAVFGLDSALIRYIAIQASRKDE
                     KGVWGTLQVGVGVATLLSVITGTFIFGLAYYLAETVFHEPRLVPLLQFTGVIVPLLTM
                     SEVLAGALRGFKRMDYPVIAQFVFQPVVRLIMIVVLAFTGFNAVHAILTFGLADLAAS
                     IMLIYFLNKEFSLRRSVRAARRDFRTIFEFSIPVWLSEMLVKFHGNIQTLILGSLNTI
                     TGVGIFSVASQISMVSSQFSSSINTSAKPVIAELHDRADMREMGQIYQTANKWAVMVQ
                     IPIFITMVLFPAPILSLFGESFTDGALALSIIALADLLKVGTGMGGIIIDMTGYTKLK
                     LVNSIFRLVANLGLDILLIPRWGLLGAAVAALIGEGFINLLRLFQVFFLFRLIPYNRT
                     FIKPVIAGVITLASAFLIKTWLILDGGLFFVIANT"
     gene            complement(1632..2780)
                     /locus_tag="AC16CB_10500"
     CDS             complement(1632..2780)
                     /locus_tag="AC16CB_10500"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HIR3342941.1"
                     /translation="MEIKAYFRILARRWWIIPCIFIPTLLATVYWTYQQPLVYETVST
                     FIIRPRSELVVDDEFVRALDMVSRRVEINTTFAEVASSQLIENLAAERLQLSPDQLKG
                     LHVSARVIGGTNLMELTVQGRDPFVVADFAGAVGIETVKYVSNLYDVFELEPLDEALI
                     PKDPVKPIFAFNIGLGAFLGLALGIGVVFLVQYIQAPHDIEMEYFNIIDRDTGAFNKS
                     YFLLRLWQEMSRAKRNKYPLSLGLIKIEYINSGNDLPSSSNQAEALRRAKILTEKILR
                     EEDILARFDTDILALLLPDMSGEKARSVMENFMSEISAIAYDVGGQDHKFYLKSSVGV
                     ASYQNYLLKQDQFLQQAIQALESANIGSNGEVILFSNNHFQKAFKSSN"
     gene            complement(3860..4567)
                     /locus_tag="AC16CB_10505"
     CDS             complement(3860..4567)
                     /locus_tag="AC16CB_10505"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR3342942.1"
                     /translation="MTTEVRFTVRQNESLVLEAIKVNHNWYLILKRILDITICILILP
                     FILVLMAVIAIAVRIDSPGSPIFVQERIGKDGRRFRMYKFRTMRHDYDPQLDRAFMQA
                     FVVGQNVINDQNHNGAYFKPTKNEHVTRLGRVLRKTSLDETPQIINILKGEMSLIGPR
                     PNLPWEVEKYQDWHYERLTVLPGITGLAQVRGRSCITFDHIVRFDIEYIHNLSIKSDL
                     QILWKTVATVLNGSGAG"
     gene            complement(4851..5186)
                     /locus_tag="AC16CB_10510"
     CDS             complement(4851..5186)
                     /locus_tag="AC16CB_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342943.1"
                     /translation="MKRILMVNSELLLSAGVESLLSNETDFGLKRIILDHEIDLGDEL
                     NRYQPDTIIMDESIMMTDPSALMGLLNNRPEMRVIVLQVRENQLQIYKRQDVPISCVE
                     DLVTVVRGG"
     gene            complement(5427..6110)
                     /locus_tag="AC16CB_10515"
     CDS             complement(5427..6110)
                     /locus_tag="AC16CB_10515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3342944.1"
                     /translation="MKLLLVDDHILFREGLKSLLGSQPGFQIVGEAATGEEAIAKTRL
                     QHPDLVLIEIGLKDGSGLETIGNILSHRQETKVVVLTLLELDETMVASIRAGAAGYLL
                     KSTPIAKLAATLRAVLDGEAALSRKMMGRILEEFSRQGTNNGKTRSNLDCLTNREIEV
                     LQYIGTGATNREIAENLFITEYTVKNHVHNILDKLELKNRREAARIARNQGLVKSVQD
                     IISGMVFDN"
BASE COUNT         1704 a         1467 c         1362 g         1760 t
ORIGIN      
        1 gtatttgcaa tcacaaaaaa taatccccca tccaagataa gccaggtttt aatcaagaag
       61 gcactcgcta acgttattac accagcaatg accggtttga tgaaggtccg gttgtacgga
      121 attaaccgaa acaggaaaaa cacttgaaag agacggagta ggttaatgaa accttcgcca
      181 atcagtgctg ccacggctgc acccaataaa ccccagcgag ggatcagcag gatatccaaa
      241 cccagattgg ctaccagccg gaaaatcgaa tttaccagtt tcagcttggt ataaccggtc
      301 atatcaatga tgattccccc catacctgtt cccactttta acaggtcagc caacgcgatg
      361 attgaaagcg caagcgcgcc atctgtgaaa ctttcaccga acaaggataa gatcggggct
      421 ggaaaaagca ccattgttat aaatatcggg atttgcacca tcaccgccca cttgtttgcc
      481 gtctgatata tctgccccat ctccctcata tctgcccggt cgtgcagttc ggcgatgact
      541 ggtttagctg aggtgttgat cgatgaggaa aactggctgc tcaccattga tatctggctg
      601 gcaacactaa aaatccctac gcctgtaatt gtgttcaatg atccaaggat tagagtctgg
      661 atgtttccgt ggaatttgac caacatctcc gacaaccaaa caggaataga gaactcaaat
      721 atcgttcgga agtctcgtcg ggctgctcgc actgatcggc ggagagaaaa ctccttgttt
      781 aggaaataaa taagcattat ggaggctgcc aagtcagcca gcccaaaggt aagtatcgca
      841 tgaacagcat tgaagccagt aaatgccaat accacgatca tgatgagcct aacaactggc
      901 tgaaagacga actgcgcgat gacagggtaa tccattctct tgaaaccacg caacgcccca
      961 gctaaaacct cgctcatggt cagtaatgga acaatcacac ctgtaaattg caacaatggt
     1021 acaaggcggg gttcgtggaa tacggtctca gccagataat aagccaatcc aaaaatgaag
     1081 gtgcctgtga tcacgctcag caaggtcgcc acacccacgc ccacttgaag ggtaccccat
     1141 accccttttt catccttgcg gctggcctga atggcgatgt agcgaatcaa ggcagaatcc
     1201 agcccgaata cagctaagcc aatggcgata ttggctgtgc tcaatgccag gttatacatg
     1261 ccatactgat ccgcccccag gaggcgtgcg acgataaccg cagtcaccag ccgaactgcg
     1321 gaaaggaaca ttttgcctgc gaatgtaatc cctccgtctt tggcaatggc aacaatgttt
     1381 cgttcagagc ttcgttctcg cgaagaactg gacatgcctg cgttgatggc cctgtcaatc
     1441 actttttcac tcatatggga ggtaccggca tagcggaatc atcgatcaat tttaattatc
     1501 atcactcaaa aatctggtgt actcttagtt atataaagag tatgactccc aacccttaat
     1561 tatcgttggt ggggtgttgg ctgcgctctc tttggttttc ctgcaagctt gcttgttaga
     1621 tctttggatg atcagttact gcttttgaat gctttttgga aatgattatt ggagaaaaga
     1681 attacttctc cattcgatcc tatgttagct gactcaaggg cttgtatagc ttgttgaagg
     1741 aactgatctt gttttagcag atagttttgg tatgaagcta cccccaccga gcttttcaag
     1801 tagaatttgt gatcttggcc ccccacatca taggcaattg cactgatttc tgacatgaag
     1861 ttttccatca ctgatcttgc tttttctcca gacatatcag gtagcaacaa tgccaaaata
     1921 tcagtgtcga agcgagccaa aatgtcctct tctcttaata ttttttctgt gagaatcttg
     1981 gcccggcgaa gcgcctcagc ttgattggaa gaagagggaa gatcatttcc tgaatttata
     2041 tactcaattt tgatcagacc caaggatagt ggatatttat tgcgtttggc gcggctcatc
     2101 tcctgccata gcctcaataa gaagtacgat ttgttgaatg caccggtatc ccgatcgata
     2161 atattgaagt attccatttc aatatcatgg ggcgcttgaa tgtattgaac aaggaaaaca
     2221 accccaattc ctaaagctaa tccaaggaat gcgcctaggc caatattaaa ggcaaaaatc
     2281 ggttttacag gatctttagg tatcagggcc tcgtccaggg gttcgagttc aaagacatcg
     2341 tatagattgc tgacatactt gaccgtctca attccaacag cgcccgcaaa atcagctacc
     2401 acaaatggat ctctaccctg tacggtcagc tccataagat ttgtaccccc aatgacccga
     2461 gcacttacat gaagaccttt caattgatcc ggggataatt ggagacgctc tgcagccaaa
     2521 ttttcaatta attggctgct ggctacttct gcgaaggtgg tgttgatttc tacgcgtctg
     2581 ctgaccatgt ctagagcgcg cacaaattca tcatcaacta ctagctcgga gcgaggtcgg
     2641 atgatgaaag tagatacggt ctcataaacc aatggctgtt gataggtcca gtaaacggtg
     2701 gcgagaaggg taggaataaa gatacaaggt attatccacc accttctggc caatatacga
     2761 aaataggctt tgatttccat aggcttaccc tctgctgatc accaaaaaac ttttccatct
     2821 agaaatattc gagctggggg atatgttatc cataatgtac ttgagcacga atctccaaag
     2881 acggttgatt tgctgctaac tggtctttaa taaagtgatt atacttctcg atttttgcgg
     2941 tcccgtgaca aagttgttag ttgcgctgtc tcacaaccac attgaaggtc actaggcatg
     3001 ctccgccatt tcgacattta gtatgtcgat tatttgcctt cttataatgt agatccagta
     3061 gtctttgtag tagggcgaca attggatccg ctttttagac acggtacccc ctctaaccaa
     3121 tttaactgga caaataataa tccatttggt tcacgttgat cttttcgatc ttgatagtcc
     3181 aataaagttc ttttgcttct tcataaacaa ccatcgttat tcactgcccg aagacaggga
     3241 caattcgaga taagccatct gtatcctaag ataaatggat cagatgcagc tatatagact
     3301 gcaaaggatt ttctcttatt tggttgttgg cgggagagac ttgcagttgt gcgactggtc
     3361 ttggttagag caaacaagtg tttcatggat gaaaatagat aaatcaattt gataatcttt
     3421 ttgctcttat tgagaccttg ccgtcctctc gctggccaca atttgttgag atacgctgaa
     3481 ctatgcagta gcataggtaa tatcagcgaa ttacatggat ggttttgttg ctgactcctc
     3541 cataacctcg ctcatgtgct ttggttcttg cccagtcggt ctgtaattgc gcacatcggc
     3601 actttaacca caatttataa aggtgcttgt tacgctccca gaggatatag caaatagcca
     3661 tcaatatgag gtagtctggg agatggttcg ttaatataaa cgccccccat agatggttgt
     3721 tacagttcca gataacaaac cttgtagtcc gcgctgatca ttctctgtgc gtggagtgga
     3781 tccagtgccc cgatgaaatt cctgggcata tgcaaatcgc tagatttccc cacccgacgc
     3841 ttttgtattg ggggattggt tatcctgctc ctgagccgtt taatacagtg gctactgtct
     3901 tccacaagat ctgtaaatca ctcttgatgc tcaaattgtg aatatattca atatcaaaac
     3961 ggacaatgtg atcgaaggtt atgcaacttc gcccgcgaac ctgagctaac ccagttattc
     4021 cgggaagaac tgttaggcgt tcataatgcc agtcctgata cttctctact tcccaaggaa
     4081 gattcgggcg agggccaatc aggctcattt cacccttcaa gatgttaatt atttgagggg
     4141 tttcatctag actggtcttg cgtaacacgc gacccagacg ggtgacatgt tcatttttgg
     4201 tgggcttaaa gtatgccccg ttgtgatttt gatcgttaat aacattctgc ccgacaacaa
     4261 aggcttgcat aaaagcgcga tcaagctgag gatcgtaatc gtgtcgcatg gtgcggaatt
     4321 tatacatgcg aaagcggcgg ccatctttgc ctattcgctc ttgaacaaag atcggtgaac
     4381 caggagaatc gatccgcacc gcaattgcaa tgacggccat cagcaccaag ataaagggca
     4441 atatgaggat gcagatggtg atatctaaaa tccgcttcaa gattagatac caattatggt
     4501 taaccttaat agcttcaaga acaagacttt cgttttggcg tacagtaaac cttacctcag
     4561 tagtcatttc agtactaact ccttatctgt ccctctgttc agcacttgta acgatgcaag
     4621 cagcaataag actatttaag aaatccgcaa ggcaccaggc atgctccgcc attacgacat
     4681 gtaagaatgt cgaccttcct ccctttgaaa cgaacccaga tgaaaaccgt tacgagtcaa
     4741 cagggttcgc ttattagaca cagcccatgc tcccagaagt ttgatagcct tttaagaagt
     4801 atgagcccag tgacccatcg aggctgtatg tcgtttgagt gatcgccaca ctatcctcct
     4861 cggaccaccg tcaccagatc ttcaacacaa gagatgggaa catcttgtct tttgtaaatc
     4921 tgcaattggt tttcacgaac ttgcaatacg atcacacgca tttcgggacg attgttgagt
     4981 aaacccatga gcgctgatgg atcggtcatc atgatgctct catccataat gatggtatcg
     5041 ggttgataac gatttagctc gtcacccaaa tcgatttcgt gatctaaaat gatcctcttt
     5101 agaccgaagt cagtttcatt agataacagg ctttcaacac ctgcagagag caagagctcg
     5161 ctatttacca tcaggattcg tttcatgttt tctcaataac ccgacaaacc tggtattaat
     5221 tgacaggatt tatgacatat tagatatgaa taatcctata attttctaat tatttcgtca
     5281 atcaatcggt atagttcctt aagatctaga aaagtactgt tttttggcag ctcccgactt
     5341 tctgataggg ggtaccgcgt tcattctgaa catgatttag aaaattttct cttgtgtcct
     5401 atggttgttt ccccttggtc aggtcatcag ttgtcaaaca ccatcccgga aatgatgtcc
     5461 tgcaccgatt tcaccagtcc ctgattgcgc gctattctgg ctgcctctcg gcggttcttt
     5521 agttccagct tgtccaagat gttatggacg tgattcttga ctgtatactc cgtgataaac
     5581 aggttttcag caatctcgcg gttcgtcgcg cctgtcccaa tatattgaag cacctcaatt
     5641 tcgcggttgg tgagacagtc taggttggag cgtgtctttc cattatttgt tccctggcga
     5701 gaaaactcct ccaggatacg tcccatcatt tttcgcgaga gggccgcttc tccatccaat
     5761 acggctctca atgttgccgc taatttagct ataggtgtgc ttttgagcag gtaacccgca
     5821 gcgcccgctc ggatggatgc gaccatcgtt tcgtccagtt caagcagggt gagtaccact
     5881 accttcgtct cttggcgatg tgataggata ttgccaatcg tttccagccc gctcccatcc
     5941 ttcaggccga tctcgatcaa tactaaatca gggtgctgta agcgcgtctt agcaatggct
     6001 tcttcaccag tggcagcctc tcccacaatt tggaagccgg gctggctgcc aagcaagctc
     6061 tttagcccct ccctgaatag tatatggtcg tcgacaagta ataatttcat gtgataaacg
     6121 atattcgatg aataaaagat tagatctggg cgaggatcat cctattagaa ttgaagatca
     6181 gatgaaaaca agaagaattc tgttgttaaa gtaaaatcca ccttaggggt ggtttcgcta
     6241 ctggctcctc gctgacctca cctacctgtt gagataggat aagcgaccaa atc
//
LOCUS       DBNGSX010000351         3494 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_654177, whole genome shotgun sequence.
ACCESSION   DBNGSX010000351 DBNGSX010000000
VERSION     DBNGSX010000351.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_654177"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="AC16CB_10520"
     CDS             <1..510
                     /locus_tag="AC16CB_10520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3342945.1"
                     /translation="DHPATMLAYRARVVVTGPAGQREIPIDEFFLGPFESSLSQDEIL
                     TEVRIPSSNGRSGGAYQKLERKVGDYATAAVAAQVTLDGAGKVASVGIGLTNVGLTPI
                     RASAAEDYLIGKAPTPENISQAASLAAEAAEPEPDYRGSEAYKRSLVRTLAHRALMKA
                     VERASGGQA"
     gene            507..1001
                     /locus_tag="AC16CB_10525"
     CDS             507..1001
                     /locus_tag="AC16CB_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3342946.1"
                     /translation="MSTHTINITVNGTLHTVDVESRLLLVHLLRETLNLTGTHIGCDT
                     TSCGACTVQVNGIPIKSCTMFAVQADGAEVKTVEGLAENGKLHPLQEGFYEKHGLQCG
                     FCTPGMLMTSSALLESNPNPTEDEIRWGISGNLCRCTGYVNIVKAVQYAADKMQQEQG
                     GSHD"
     gene            994..3345
                     /locus_tag="AC16CB_10530"
     CDS             994..3345
                     /locus_tag="AC16CB_10530"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043885 - anaerobic carbon-monoxide
                     dehydrogenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HIR3342947.1"
                     /translation="MTKEVMGLGHSRKRVEDARFLRGKGNYIDDFKLPGMLHLDIVRS
                     PYAHAKIKSINGEKATAIPGVLAVIDGKLLQQYNVHNMPTLMSDVQNVLPIDTVKYQF
                     QEVAAVLATDRYTAADGVAAVEVDYEPLPVMVDPKKALEPGAMKVREDKGSNHIWHWE
                     WGNREATNADISAAEVVVKEDIYIPRIHVSSIETCGCVAKWDSVEGHLTLWMTTQAPH
                     AIRTVVALITAHLGLSEEKIRVISPDIGGGFGGKVPAYPGYVIAVAASIVTGAPVKWI
                     EDRTENLAADSFGRDYHITAELAGKRDGTITSLRIKTIADHGYTDAAANPSKFPAGLF
                     HICTGSYDLKNAFVEVDGAYTNKPPGGIAYRCSFRVTEAVHTIERMADLYAHEIGMDP
                     ADFRMKNFIKPEQFPYKSALGWEYDSGDYHSALRLAMDIVGYEELRKEQEEKRARGEF
                     MGIGISSFTEIVGAGPSKHFDILGLKMFDSCEIRVHPTGKAIARFGTKSQGQGHETTY
                     AQIIAEELGIPADHIKIEEGDTDTAPYGLGTYASRSTPTAGAAAAIASRKIRDKARKI
                     AAHLLEAAEEDLVWEQGKFYVKGSPDQAKTIQEIAFAAYTNHPQGMEAGLEAVNYYDP
                     PNLTYPFGSYVAVVDIDRGTGEVKVRRFVAVDDCGVRINPMIVEGQIHGGLAMGFAPA
                     MMEEIQYDENGNIYGSTFMDYLLPTAVETPKWELGETVTPSPHHPIGAKGVGESATVG
                     APAAIVNAVVDALWHIGVRHVDIPIKPSRVWELLNEHGAAEYK"
BASE COUNT          833 a          956 c         1013 g          692 t
ORIGIN      
        1 gaccatccgg caacaatgct ggcatatcga gccagagtgg tcgtcactgg accggcgggt
       61 cagcgagaga tccccatcga tgaattcttc ctggggcctt ttgaatcatc cctttctcag
      121 gacgagattc tcaccgaggt ccgcattcca tcttcgaatg ggcgatctgg gggcgcctac
      181 cagaagctgg agcgtaaggt cggcgattat gccactgcgg ctgtagctgc acaggtcacg
      241 ctcgatggcg cggggaaagt ggcatccgta gggataggcc tgaccaatgt cggcctaaca
      301 cccatccggg caagcgccgc ggaggactac cttattggga aggctcccac cccggaaaac
      361 atctcccagg ctgcatctct ggcagccgaa gcggcagaac cggaaccgga ttatcgaggt
      421 tcggaagcgt acaagcgttc actagtaagg acactcgcac accgcgctct gatgaaagcg
      481 gttgaacgag caagcggagg tcaggcatga gtacacatac cataaacatt actgtcaacg
      541 gaacattgca tactgtagac gtggagtcac ggctgttgct ggtgcattta ctgcgggaga
      601 ccctgaactt gacgggtacc catatcggct gtgacaccac ctcttgtggt gcctgcacag
      661 tccaagtgaa cgggattccg attaagtctt gtaccatgtt cgcggtccag gcggatggtg
      721 ccgaggtaaa gacagttgaa gggcttgctg agaatggcaa gctacatccc ctgcaggagg
      781 gtttctatga gaagcatggc ctgcagtgcg ggttctgcac accaggaatg ctgatgacct
      841 catcggcgct gttggaatcg aaccccaatc cgaccgagga tgagatccgc tggggaattt
      901 ctggcaacct gtgccggtgt acaggttatg tgaacatcgt gaaagccgtt caatacgcag
      961 cagacaagat gcagcaggaa caaggaggca gccatgacta aagaggtaat gggcttgggc
     1021 catagccgga agcgagtgga agacgcgcgc ttcctgcgcg gcaaaggaaa ttatattgat
     1081 gactttaagc tgccagggat gctgcacctc gacatcgtcc gcagcccgta tgcccacgcg
     1141 aaaatcaaga gtatcaatgg cgagaaggcc acggcgatac ctggcgtact cgccgtaata
     1201 gatggcaaac ttctgcaaca atacaatgtg cacaatatgc caacactgat gagcgacgtc
     1261 cagaacgtgc tgcccatcga cacggtcaaa taccagttcc aggaagtcgc ggctgttctg
     1321 gcgacagacc gctacactgc agccgatggt gtggccgcag tggaggtgga ttacgaaccg
     1381 cttcccgtca tggtggatcc gaaaaaagca ttagaaccgg gcgcgatgaa agtgcgggaa
     1441 gacaagggca gcaaccacat ctggcactgg gaatggggta accgcgaagc aactaatgcg
     1501 gatatcagcg ccgcggaagt ggtcgtcaaa gaggatatct acatcccccg tatccacgtc
     1561 tcctccatag agacctgcgg atgcgtggcg aagtgggatt ctgtcgaggg gcatctcacc
     1621 ctgtggatga ccactcaagc gccgcacgcc atacggacag tggttgcgct cattacggcc
     1681 cacctggggc tgtcggagga aaaaattcga gtgatctcgc ccgacattgg tggcggtttc
     1741 ggtggtaagg tcccggcgta tccaggctat gtgattgctg tggcggcttc gattgtgacc
     1801 ggcgcgccgg ttaaatggat cgaggaccgc accgagaacc tggcagccga ttcattcggg
     1861 cgcgattatc acatcactgc agagctggcc gggaagcgtg acggaacgat tacctcgcta
     1921 cgcatcaaga ccattgccga tcacggctac acggatgctg cagctaaccc gtccaagttc
     1981 cctgccggtc ttttccatat ctgcaccgga tcctatgacc tgaagaacgc ctttgttgag
     2041 gtggatggag cctatacaaa taaaccgcct ggcgggatcg cttaccgctg ttccttccgg
     2101 gtgaccgagg ctgtccacac cattgagcgt atggcggacc tgtatgccca cgagattggc
     2161 atggaccccg ctgatttcag gatgaagaac ttcatcaagc cagaacagtt cccgtataaa
     2221 tccgcgcttg gatgggagta tgacagcggt gattaccaca gcgccctgcg cctggccatg
     2281 gacatcgtgg gctatgaaga gctacgcaag gagcaagaag agaaacgcgc ccgtggtgag
     2341 ttcatgggca tcgggatctc ttccttcacc gagatcgtcg gcgccggacc atccaagcat
     2401 tttgacattc tcggcttaaa aatgtttgac tcctgcgaga tacgcgtcca cccgactggc
     2461 aaagccatcg cacgctttgg caccaagtca cagggtcaag gacacgaaac tacttatgcg
     2521 cagatcatcg ccgaggagct gggtatccca gccgatcaca tcaagatcga agaaggcgat
     2581 acggatactg ctccatatgg tctcggcacg tatgctagcc ggagcacccc taccgctggc
     2641 gccgcggccg ctatcgcttc tcgcaagatc cgggataaag cccgaaagat cgctgctcac
     2701 ctgcttgaag ctgccgagga ggaccttgtc tgggaacagg gaaagttcta cgtgaaaggc
     2761 tctcccgacc aggcgaagac catccaggag atcgcttttg ctgcatacac caaccatccc
     2821 caggggatgg aagccggttt ggaagcggtg aactactatg acccacccaa cctgacgtat
     2881 cccttcggtt cctacgtcgc tgtggtggat attgataggg gcactggcga agtcaaagtc
     2941 cgtcgatttg tagcggtgga cgactgcggt gtgcgcatca atccgatgat cgtggaaggg
     3001 cagatccacg gcgggctggc catgggtttt gcccccgcca tgatggaaga gatccaatac
     3061 gatgaaaacg ggaatatcta tggcagcacc ttcatggact acctgctgcc aaccgctgta
     3121 gagaccccca aatgggagct gggcgagaca gtcaccccgt caccccacca ccccatcggg
     3181 gcaaaaggcg tgggagaatc tgcaacggta ggcgcccccg ccgcgattgt aaacgctgtg
     3241 gtagatgcgc tctggcatat tggggttcgc cacgtggata ttcccatcaa gccttcccgg
     3301 gtttgggagc tcctcaacga gcatggggca gcagaatata agtaactctc aataaatcat
     3361 cccccggagt ggctgagcac cccgggggat tacaggctcc agttattatc acagaacacc
     3421 tcgtactcat aaattctctt gccgagcagg gtgagccctt tgcaactgcg gtcgtcgtgc
     3481 gtgcggaaaa gcca
//
LOCUS       DBNGSX010000352         3263 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_515602, whole genome shotgun sequence.
ACCESSION   DBNGSX010000352 DBNGSX010000000
VERSION     DBNGSX010000352.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_515602"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="AC16CB_10535"
     CDS             complement(<1..219)
                     /locus_tag="AC16CB_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342948.1"
                     /translation="MGDARPNNPDIPLECVTPRNKNGLFDFQVKVAVLTDGADIVFSH
                     PKAKCRVLEQNGSDLFGFENSIYALNWFD"
     gene            546..2051
                     /locus_tag="AC16CB_10540"
     CDS             546..2051
                     /locus_tag="AC16CB_10540"
                     /inference="COORDINATES: protein motif:HMM:NF014453.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR3342949.1"
                     /translation="MDTTINVNEIYVDLKTNFITPLRDLIKRGLDIFAAVIGILIFWP
                     FVVLVAILIKIEDPGPVLYRGRRVGMGGKIFKILKFRTMYERPESYLGSPITASDDDR
                     ITPIGRWLRDTKLNELPQLWNVLNGDMSLVGPRPEDPEIFLTLPENYREKIVSVRPGI
                     TSPASIMYHDEEKILTSKNLMDDYAELILPNKMRMDLLYVRYHNLINDFDTLFWTFAI
                     LIFRIGKQNIREGWLYNGPIARFVRHYLNWFMIDFITASAGIVSVGLGWRLFEPLDLG
                     ISNALILGIILAFSFSISNSILEVNLISWQQAGAGDSIKLIISCLLVGIFYIILNVYV
                     GIIPQLTTLFIAIACLVVLGAFVGVRYRSRLIMGIATRWINSRQSSIGVYERALIIGA
                     GNAGKFTTWTLKHPDFRHFYKVIGYVDDAPGKQGMRFDGIKVLGTTADIPNLVTKHNI
                     GLLFYSIRDANETGKARILSICRQVELPIVSVPDVIDNMLHLFAQSMKSID"
     gene            2083..2313
                     /locus_tag="AC16CB_10545"
     CDS             2083..2313
                     /locus_tag="AC16CB_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342950.1"
                     /translation="MSFDWKNITFLATDAGSLFGSHFVETLVNRGESAPGAEREVTLP
                     LYALMAEVDVRAVVSAAREALGESGLNQLDFM"
     gene            2314..3186
                     /locus_tag="AC16CB_10550"
     CDS             2314..3186
                     /locus_tag="AC16CB_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3342951.1"
                     /translation="MTDTLLNQFDDSDWSLIIEPRRKLLNLHLRELWRYRDLVLLFVR
                     RDFVSVYKQTILGPLWYLIQPLLTTLTFTVIFGNIAGLPTDGLPQFLFYMSGTVIWAY
                     FADCLNKTANTFAGNASLFGKVYFPRLAVPVSILISNLITFFFQFALFLLFVIFFAVR
                     GAPIQLHWGWVALSPLLVLMMAGLGLGFGIIISSLTTKYRDLRFLVSFGVQLFMYATP
                     VIYPASSIPARFKWIVQANPMTPVVESFRYAFLGNGTVSYFQLLYSFAFMLVVVFLGS
                     LVFNRVEQTFMDTV"
BASE COUNT          761 a          767 c          798 g          937 t
ORIGIN      
        1 atcgaaccaa tttaatgcgt aaattgaatt ttcaaaacca aacagatctg atccgttttg
       61 ctctaaaacg cggcattttg ccttcggatg gctaaataca atatcggcac catctgtcaa
      121 aacggccact ttgacctgaa aatcgaaaag gccgttttta tttctaggag ttacgcactc
      181 caggggaata tcggggttat tagggcgggc gtcgcccacc ccaataaccc caacctcccg
      241 tcaactgcgt aagccctcat ttctttacaa aacgcttatg caaattacgg gctatttttg
      301 ctaaaaacgc gtagatttac ggataggaaa atgcgccaca cttcagtatg ctaacccaca
      361 cgaggtctgg ctgcggaagc cgctgacggc tttgcaattg aggtatcaat tcttcagtgt
      421 ccacacttca actgaaatgt cggaagggcg gagccattaa taatgcggtt gaaacgcatt
      481 gtacaacgag cttcagcaaa atcatcgcat acagtttttt ttgatcccaa agggttatac
      541 acattatgga tacgactatt aatgttaatg aaatctatgt agatttgaag acgaatttca
      601 tcacaccgtt aagggatctt atcaagcggg gcttggatat ttttgcagcg gtgataggaa
      661 tattaatctt ttggccattt gttgttctgg ttgcgattct gatcaaaatc gaggatccag
      721 ggccggtgtt gtaccgtggt cgccgtgttg ggatgggcgg aaagattttc aagattctga
      781 agtttaggac aatgtatgag cgccccgaaa gctatctggg ctcacccatt acagccagcg
      841 atgatgaccg cattaccccc attggtcgtt ggctgcgaga taccaaattg aacgaacttc
      901 cgcaactttg gaatgtatta aatggtgata tgagcctggt agggccccgg cctgaagacc
      961 cggaaatctt tcttacactg cctgagaatt atcgcgaaaa aatagtatct gttcgccccg
     1021 gaattactag tccggcgagc attatgtatc acgatgaaga gaaaatttta acttcaaaga
     1081 atttgatgga tgattatgca gaattaattt tacccaataa aatgcgcatg gacctgttgt
     1141 acgttcgata ccataatctt atcaatgatt tcgacaccct tttctggaca tttgccattc
     1201 ttatcttccg gattggcaaa caaaatattc gggaaggctg gttatataac ggtccgattg
     1261 cccggtttgt gcgtcactat ttaaactggt ttatgatcga ttttataacc gcttcggcag
     1321 gaattgtttc ggtagggctt ggatggcggt tgtttgagcc cctggatttg ggaatatcaa
     1381 acgccctcat ccttgggatc atcctggcat tttcattcag cattagcaat tctatattgg
     1441 aagtaaatct gatttcctgg cagcaggcag gggctggtga ttcaatcaag ttgattattt
     1501 cttgcctgct tgttggcatt ttttacatta ttcttaatgt atatgtcggg attattcccc
     1561 agcttaccac tctcttcatt gcaattgctt gcctggtagt tctcggagca tttgtagggg
     1621 tacgctaccg gtcgcgcttg atcatgggca ttgccacacg ctggattaat tctcgtcaaa
     1681 gcagtatcgg ggtatatgaa agggccctta tcataggagc tgggaacgca ggcaaattta
     1741 cgacctggac gttgaagcac ccggatttcc ggcatttcta taaggtgatc ggttatgtgg
     1801 acgacgctcc cgggaaacag ggtatgcgat ttgatgggat caaggtatta gggacaactg
     1861 cagatatccc aaatctggtt acgaagcaca acatagggtt gcttttctat tcgattcgag
     1921 atgcaaatga aacgggtaag gctcgcattc tttcgatctg caggcaggtg gaattgccga
     1981 ttgtatctgt tccagatgtg atcgataaca tgctccattt gttcgctcaa agcatgaagt
     2041 ctattgatta aaactttcag ccggatatca ggtttcatat atatgtcatt tgattggaaa
     2101 aatataactt ttctcgcaac cgacgcaggc agtcttttcg gcagccactt cgtcgagacg
     2161 ttggttaacc gtggagaaag cgctccaggc gctgaacgcg aggtgacctt gcccttgtac
     2221 gccctgatgg cagaagtcga tgtgcgagcg gtggtgagcg ccgcgcgcga ggcgctgggc
     2281 gagagtggct taaaccaatt ggatttcatg tagatgacgg acacccttct caaccaattt
     2341 gatgatagtg actggtcgct gatcattgag ccgcggcgca agctgctgaa cctgcacctg
     2401 agggaactgt ggcgctaccg cgacttggtg ctgctgtttg tgcgccgtga ctttgtgtct
     2461 gtctacaaac agaccatcct tggcccgctg tggtacctga tccagccgtt gctcactacc
     2521 ctcaccttca ccgtgatctt cggcaatatc gccgggctcc ccacggacgg cctgccgcag
     2581 ttcctgttct atatgtctgg cacggtcatc tgggcttact tcgccgattg cctcaacaaa
     2641 accgccaata cttttgccgg caatgccagc ctgtttggca aggtttactt cccacggttg
     2701 gcagtgccgg tttctatcct gatctccaac ctgatcacct tcttcttcca gtttgctctg
     2761 ttcctgctgt tcgtgatctt ttttgcagtg cgcggcgcgc ctatccagct acactggggc
     2821 tgggtggcgc tctcccccct gctggtgctg atgatggccg ggctgggctt gggcttcggc
     2881 atcattattt catcgcttac caccaagtac cgtgacctgc gtttcctggt ctcttttggt
     2941 gtgcagctgt tcatgtatgc cacaccggtc atttacccgg cctcatctat cccggctcgt
     3001 ttcaaatgga tcgttcaggc caatccgatg acccctgtcg tggagtcatt ccgttacgct
     3061 ttcttgggca acggtacggt gagctacttc cagctgctgt acagcttcgc cttcatgctg
     3121 gtggtggtct ttttgggcag cttggtcttc aaccgggtgg aacagacgtt catggatacg
     3181 gtgtgaagac tatggccatt agaagaaatt cgaagtgtcc aaagaactat ctcaaaagac
     3241 ttcccagcgg tttaaggtca ggt
//
LOCUS       DBNGSX010000353         5678 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1900799, whole genome shotgun sequence.
ACCESSION   DBNGSX010000353 DBNGSX010000000
VERSION     DBNGSX010000353.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5678)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5678
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1900799"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="AC16CB_10555"
     CDS             complement(<1..1100)
                     /locus_tag="AC16CB_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3342952.1"
                     /translation="MPQSLDEALTYSNRWIDIIHKTEIPLDEGKQIIEESKNNFAEHF
                     NKGWLEYRKSVTEAGDWAATEWTGSGAVFRDLIGREYIDCLGGYGMMDLGWGHPDVVA
                     AVRAQLNRTPMPSQELIDPLRGVLARLMANITPGDLKYSFFAGSGTESIEGAIKLAKM
                     YTKKNGFIVAVKAFHGKTLGSLSMMGKFDFRQPPGILYGGPIYHVPFGDADAVEQQLD
                     ICKKVGVDLAAVLFEPIQGEAGAIVPPDDFWPRVRELTKHYGVLLIADEVQTGMGRTG
                     KLWGLDHWGVVPDILAVAKSLGGGVMPVSAFCSTEEIWQCMMYPNPFIHTTTTGGGAL
                     ACSAAIASIHVTLRDRMWEQAAEKGDYLIPKL"
     gene            complement(1122..2441)
                     /locus_tag="AC16CB_10560"
     CDS             complement(1122..2441)
                     /locus_tag="AC16CB_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3342953.1"
                     /translation="MITERTTLTSQEIVQLNRQYTFFSWSVQSAVNPIPVTRAEGIYF
                     WDADGKRYIDFSSQLMNMNIGHQHPKVVGAIQEQASQLCFVHPGLATEARGKLGKKIS
                     EVAPGHLNKTFFCLGGAEANENAIKLARLYTGRNKILARYRSYHGATHGAMALTGDYR
                     RLAVEPTMPGAVHFLDPYCYRCPFGWTRETCHRECISHIEEIIQYEGPDHIAAIIMEG
                     VTGSNGLIVPPDDYWPRVREICDKYDILLISDEVMSGWGRTGKWFAVDNWEVVPDMIT
                     TAKGLTSGYVPLGAVIVSDDIAEYFEDKMLWAGLTYSGHALACAAGLATLEVYQEDGL
                     IENAVKVGRYLGERLEAIKLSHPSVGEVRYIGLFSALEIVKNRETKEPIDPLTEVAKY
                     LKEHGLFTFVFHNMLFIVPPLCITEAQLDEGLEIIEQGLLITDAQTR"
     gene            complement(2573..3877)
                     /gene="gntE"
                     /locus_tag="AC16CB_10565"
     CDS             complement(2573..3877)
                     /gene="gntE"
                     /locus_tag="AC16CB_10565"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanitoxin biosynthesis PLP-dependent
                     transaminase GntE"
                     /protein_id="HIR3342954.1"
                     /translation="MAIQREKSNSLQRRAMAVTPLGVNSNFRYWGDGITPYVLKAKGG
                     HLWDVDGNRYIDYRLAYGPIILGHAYDEVDAKVHQEIDRGVLFAMTSELEIAVIEKIV
                     EMCPGVEMARLACSGTEATMHAIRVARAYTGREKIIKFEGMYHGFQDYTLFSTYAPVE
                     AYGNYRSPIPIPASSGIPRALHDLVITLPFNEPEILDMILRRVGNDVAAIIIEPCMGN
                     CGAILPKKGFLEFIRAKTSEYGIVFILDEVKTGFRIARGGAQEYYNVIPDMATYAKSI
                     GNGYPIAAFGGKREIMQTIGRGVAQGGTFNNNKPGVAAAFVTLDLMQKLPVHETISKR
                     GQRLMDGIEKIFQVAGIPACINGYPAMFSFAVGLEKVTNQRDWSESDRTYYLRLVEAL
                     VERGVMPDHDPREPWFLCYSHSDADIDETLNAMDDAVRHTLR"
     gene            complement(3924..4400)
                     /locus_tag="AC16CB_10570"
     CDS             complement(3924..4400)
                     /locus_tag="AC16CB_10570"
                     /inference="COORDINATES: protein motif:HMM:NF046293.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR3342955.1"
                     /translation="MSKIDKKDLQIVNLLMEDGRMSAAEIARQVGNISERVVRYRIDR
                     MVKEGIISINAIPKPKALGFTVIADVFIEVETGRIQEVATKLKDMECVSYVACAIGET
                     DISVQLVAHDTGEVYQFVTEVIGRTPGVRKTTTSIVPYTLKDVYQWRIPPSVSVDD"
     gene            4738..5220
                     /locus_tag="AC16CB_10575"
     CDS             4738..5220
                     /locus_tag="AC16CB_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342956.1"
                     /translation="MESGNQSEEINTISEEDKLSPQELRRQRQAIIGIVAIAIILIAA
                     VVVSVILLLQPTTPTDRIRDIFIIFMAIESLLLGLVLVILIAQLARLINLLQNEIKPI
                     LDSTNQTVSTLRGTTAFLSDNIVEPVMKMNEYFAGVQQALRVIGLARGKRRNSNIQGE
                     "
     gene            5224..5673
                     /locus_tag="AC16CB_10580"
     CDS             5224..5673
                     /locus_tag="AC16CB_10580"
                     /inference="COORDINATES: protein motif:HMM:NF024143.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HIR3342957.1"
                     /translation="MSDRDGEFGAYLAGFMVGGLVGAAVALLFAPQSGEETRTIIREK
                     SIELKDKAYETAEEARTRAEKALEDARTRAETAYEDVRTRAEELAAITKERATEVQKR
                     GQVVLEDQKTRIEGAVEAGKKVAQRKRQTTKEDTASDTPPAEAPAKS"
BASE COUNT         1517 a         1519 c         1222 g         1420 t
ORIGIN      
        1 ttcaattttg ggatgagata atcgcccttt tcagcagctt gctcccacat tcgatcgcgc
       61 aatgtgacat ggatcgaggc tatcgccgcg ctgcacgcca aggcaccccc tccagtagta
      121 gtggtatgga taaagggatt gggatacatc atacactgcc aaatctcctc ggtcgagcaa
      181 aacgcgctca ctggcataac ccctccaccc aacgatttcg ctaccgcaag gatatccggc
      241 actacccccc aatgatctaa gccccaaagc tttcccgtgc gccccatacc ggtttggacc
      301 tcatcggcga tcagcaaaac gccgtaatgt ttggtcagct cgcgcacgcg cggccaaaaa
      361 tcgtccggtg gaacaatagc cccggcctct ccctggatgg gttcaaacaa caccgcagcc
      421 aggtcgacgc ctaccttctt acaaatatca agctgttgct ctacagcatc tgcatcacca
      481 aagggcacat ggtatatcgg tccaccatat agaatccccg gtggctggcg gaaatcgaac
      541 ttgcccatca tgctcaacga tcccaaggtc ttgccatgaa aagccttgac cgccacgata
      601 aacccgttct ttttggtata catttttgcc agcttgatgg ccccttcgat ggactccgtt
      661 ccactacctg cgaaaaagct gtactttaga tcaccaggag taatatttgc catcaatctc
      721 gccagtacgc cccgcaatgg atcaatcaat tcctggctgg gcatcggagt gcggtttaat
      781 tgggcacgca ctgcggcaac gacatctgga tgtccccacc ccaaatccat catgccatac
      841 ccccctaggc agtcaatgta ctcgcgaccg atcaggtctc gaaacactgc acctgagcca
      901 gtccattctg tagctgccca atcgccagcc tcggttaccg atttacgata ttccagccac
      961 cccttattaa aatgctcagc aaaattattc ttactttcct cgataatttg cttgccctca
     1021 tccaggggga tctcggtttt atggataatg tcgatccatc gattcgaata ggttaatgct
     1081 tcatcaagcg attgaggcat aaatatgctc cgtatatata tctacctggt ctgtgcgtcg
     1141 gtaattagca atccctgctc aataatctcc aagccctcat cgagctgcgc ttctgtaata
     1201 cataaaggcg gtacgatgaa aagcatatta tgaaagacaa atgtaaataa gccatgttcc
     1261 ttgagatact tggctacctc agtcaaggga tcgattggct ctttggtttc tcgatttttg
     1321 acaatttcca atgctgagaa tagtccgata tagcgcacct cccccactga tggatgactc
     1381 agcttaatag cttccaagcg ctcgcccaaa tagcgaccta ctttgacagc attttcgatc
     1441 aagccatctt cctgatacac ttccaaagtc gccagtccag ccgcgcatgc cagggcatgc
     1501 ccgctatagg taagcccggc ccaaagcatc ttatcttcaa aatactctgc gatatcatct
     1561 gagacgatca cagcacccaa aggaacatat ccagatgtca aacccttagc tgttgtgatc
     1621 atatcgggca cgacttccca gttatctact gcaaaccatt tgccggttcg cccccaccca
     1681 ctcatgacct catcagaaat gagcaaaata tcatatttgt cgcagatttc ccttacccgc
     1741 ggccagtaat cgtcaggagg gacgatcagt ccattcgaac cagtcacccc ctccatgata
     1801 atagctgcta tgtgatcggg tccttcgtac tggataatct cctcgatgtg actgatacat
     1861 tctcgatggc aggtttcccg cgtccatcca aaaggacagc gataacaata aggatccaag
     1921 aaatgtacag caccaggcat cgtcggctca acagccagtc ggcgataatc acctgtcaga
     1981 gccatcgctc cgtgcgtcgc gccatgataa gaacgatagc gcgcaaggat tttattccga
     2041 cctgtgtaca gtcgagcgag cttgatggca ttctcgttgg cttctgcgcc acccaaacag
     2101 aaaaaggttt tgttcaagtg tcccggggca acttcggaaa tctttttccc cagctttcct
     2161 cgcgcttccg tcgccagtcc agggtgcaca aagcacaatt gtgaggcttg ttcttggata
     2221 gcccccacca ccttggggtg ctgatggccg atattcatgt tcatcagctg agatgaaaaa
     2281 tcaatataac gcttaccatc tgcatcccaa aaatagatac cctcagcacg agttactgga
     2341 attggattga cagccgactg caccgaccaa gagaagaaag tgtattgccg gttaagctgg
     2401 acaatttcct gcgaagtcaa agtagttctt tcagtaatca cacgaacctc cgtaatcagt
     2461 tcagaactaa caaaaaccca gaagtagttg gggggatatg agctaccgaa acccgctcaa
     2521 atcccaatct ctccgtcaaa tatgtaaagg ctactctagt tcggatgaat aattatctaa
     2581 gcgtatggcg aaccgcgtca tccatcgcat ttaaagtttc atctatgtct gcatcgctat
     2641 gggaatagca caaaaaccac ggttcgcgcg ggtcgtggtc tggcattaca ccacgctcta
     2701 caagcgcctc aacaagccgt agatagtagg tccgatcgct ttcactccaa tctcgttggt
     2761 tagtcacttt ttcaagcccg actgcgaaag agaacatcgc tggatacccg ttaatgcagg
     2821 ctggaatacc agctacttgg aatattttct caatgccgtc cattaaccgc tgtcctcgct
     2881 ttgagatggt ttcatgcacc ggcagttttt gcatcaaatc aagggtgaca aacgctgcag
     2941 ccacgccagg tttgttgttg ttgaatgtcc caccctgggc aactccccgt ccaatggttt
     3001 gcataatctc acgcttgcca ccaaaagcag cgattgggta gccattccca atagactttg
     3061 catacgtagc catatcggga atcacattgt aatattcttg tgccccacct cgggcaatgc
     3121 ggaaccccgt cttgacctca tccaggatga agacaatacc gtactcactg gttttggcgc
     3181 ggatgaactc cagaaaccct tttttcggta atatcgcccc gcaatttccc atgcatggtt
     3241 cgatgattat tgcagccaca tcgttgccga ctcgtcgtaa gatcatgtcc agaatttcgg
     3301 gttcattaaa gggaagtgtt atgacgagat catgcaatgc ccgggggatt cctgagctgg
     3361 ccggaattgg gattgggctg cggtaatttc catacgcttc aaccggcgca taagtcgaaa
     3421 acaatgtata gtcttgaaag ccatggtaca tcccctcaaa cttgatgatc ttttcccgcc
     3481 ctgtatatgc tctcgccaca cggatagcat gcatggttgc ctcggtgcca gagcatgcta
     3541 acctggccat ctcaacacca gggcacattt ccacaatctt ttcaataact gcgatctcca
     3601 gctcgcttgt catagcgaac aggactcccc gatcaatctc ttgatgtact ttcgcgtcaa
     3661 cctcatcgta ggcatggcct aaaattatgg ggccataagc caggcgataa tcaatatacc
     3721 ggtttccatc gacatcccac aggtgccctc ctttggcctt taacacgtaa ggcgtaatgc
     3781 catctcccca ataacggaag ttcgagttga cacctaacgg tgtaacagcc attgccctgc
     3841 gttgtaacga attgcttttt tcacgctgga tagccattat ttctccaaaa tcaaatcatc
     3901 atccgcaagt aaccaattcc cgcctaatca tcaacgctca ccgagggtgg aattcgccat
     3961 tggtatacat ccttcaatgt atacggtacg attgaagttg tggttttgcg aacccctggg
     4021 gttctcccta tcacttccgt gacaaattga taaacctccc ccgtatcatg tgcaacaagc
     4081 tgcacgctga tatcagtctc accaattgcg cacgccacat agctgacaca ttccatatct
     4141 ttcagcttgg tagccacttc ctggatccga ccagtctcaa cctcgataaa cacatctgca
     4201 atgacggtaa atcccaatgc cttaggtttt gggatagcat tgatcgaaat aatcccctcc
     4261 ttaaccattc gatctatacg atagcgtaca acccgctcgg agatgttacc cacctgacgg
     4321 gcaatctcgg ctgccgacat gcgtccatct tccatgagaa gattgacaat ttgcaggtct
     4381 tttttgtcga tttttgacat agattttaat attgttttcc atttacgaca acgagagacg
     4441 aaaataattt accagattta ttaatcactg tcaatagatt taccaaaaaa ctcggcaagg
     4501 gatgaaaatt acgctcattg gtaatgtctt gtatctcgat aaagtgacgc attgccatat
     4561 gtatcctctc ctgcaaatct atgcactaag acttagatat acagatctgg cggacgactt
     4621 tgcatagaag ttgatatcaa taaacaaatt ggtcgtgaat tcgaagtttg tagatagtca
     4681 cttgaggcac tctgcctaaa tcccatacag catcaattgt ggtaaaatcc atagtatatg
     4741 gagagcggga accaatcgga ggaaattaac acaatttctg aggaagataa actttctcct
     4801 caggaactac gccgccagcg ccaggcaata attggtattg tggcaatcgc gataatcctg
     4861 attgctgcgg ttgtagtcag tgtaatctta ttactgcaac ccaccactcc cacagaccgc
     4921 attcgagaca tatttattat atttatggcg attgaatcgt tgctgctggg gttggtgctg
     4981 gttattctca tcgcccaact tgcgcgattg atcaacctgc tccagaatga gatcaaacca
     5041 atactggatt ctacaaacca gacggtaagc accttgcgag ggacaaccgc atttcttagt
     5101 gataacatcg tcgagccagt catgaaaatg aacgaatact tcgccggagt acagcaggca
     5161 ctgagggtaa ttggtctggc tcgtggcaaa cgcagaaatt ctaatatcca aggagagtga
     5221 tttatgtctg atcgtgatgg cgaatttgga gcctatttgg cagggttcat ggttggcggg
     5281 ttagttggtg ccgcagttgc cctgcttttt gccccccaat cgggtgaaga gacccggacc
     5341 ataatccgcg agaaaagcat cgagttaaaa gacaaggcct acgagaccgc cgaagaagcg
     5401 cgcacgcgcg ctgaaaaggc tcttgaagat gcacgtacac gggctgaaac agcctatgaa
     5461 gacgtacgta ccagggctga agagctagct gcgattacca aagaacgcgc tactgaggtc
     5521 cagaaacgcg gccaggttgt gctggaagac caaaaaacgc gcattgaagg tgcagttgaa
     5581 gctggtaaga aagttgcgca acgaaagcgc caaacaacca aagaagacac agctagcgac
     5641 accccaccag cagaagcacc tgcaaaatca taatattt
//
LOCUS       DBNGSX010000354        12274 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1069901, whole genome shotgun sequence.
ACCESSION   DBNGSX010000354 DBNGSX010000000
VERSION     DBNGSX010000354.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12274)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12274)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12274
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1069901"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..211)
                     /locus_tag="AC16CB_10585"
     CDS             complement(86..211)
                     /locus_tag="AC16CB_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342958.1"
                     /translation="MGQGKKGPTKREIRRVRTQQIIFALIAVIVILSWVISLVSN"
     gene            complement(230..2092)
                     /gene="uvrC"
                     /locus_tag="AC16CB_10590"
     CDS             complement(230..2092)
                     /gene="uvrC"
                     /locus_tag="AC16CB_10590"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIR3342959.1"
                     /translation="MDLQSNLQAILDTLPTKPGCYLMKDEAGKVIYVGKAINLRNRVR
                     SYFHDSAQHERRILKLVRKIRDIEWIVVDSELEALILEMNLIKKHRPHYNVRLKDDKR
                     YPYIKVHWGNPFPKITVTRQMVQDGSRYYGPYTSVWAVHQTLDVLRRIFPYLTCDRVI
                     TGNDERACLYYDIKLCTAPCIGAIDQANYQRMIDDFCHFLMGRTEPVVTRLQAEMERA
                     SEALRFEQAAVIRDQLRAIENVVARQRVISSEYVDSDVIAIARADGEACVQVFFIRGG
                     KLIGREYFLLEGTEETPDAGVMAEFIKQFYDQAPSVPQHVLLPHEVEEAQIITQWLNQ
                     KSSGQKVEILVPREGAQHELIEMALQNATETLAALQAQWHADTVRQEQALAELQSALS
                     LTEPPNRIECYDISNTQGTNAVGSMVVFERGVPKKSLYRHFNIRSVAGPDDFASMEEV
                     LTRRFNRWQAAQEASQKPGKRPDTSFSRLPDLILIDGGKGQLGRATAVFDRFGLLGKI
                     PVAGLAKQNEELFVPGRSDPIILSRQSQGLYMIQRVRDEAHRFAITSHRKLRTKQGLA
                     SRLDSIPGIGPARRKLLLNHFGSIQDIQAASMEELTAVSGITSKLAQAIKEHLE"
     gene            2226..2705
                     /locus_tag="AC16CB_10595"
     CDS             2226..2705
                     /locus_tag="AC16CB_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HIR3342960.1"
                     /translation="MDSILLELQIGEILTQRRLTLATAESCTGGLVSHRITNVPGSSH
                     YFLGGVVAYANQTKMALLDVRLDTLEKFGAVSEATVLEMARGVRQALNADIGVAVSGI
                     AGPGGGTPEKPVGLVWIGISGWGEDQARSYVWSGDRMAVKEHSAQAALEMLLSMLDK"
     gene            complement(2716..4509)
                     /gene="metG"
                     /locus_tag="AC16CB_10600"
     CDS             complement(2716..4509)
                     /gene="metG"
                     /locus_tag="AC16CB_10600"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR3342961.1"
                     /translation="MTKNILVAVAWPYANSLIHVGNITGSYLPADIFARFQRLRGNRV
                     LMVSGSDAHGTPVSVRADAENSTALQVYQKYHAGFIELFQKLGLAYDLFTSTHTENHF
                     KVSQAIFLALKNNGYLYTERQLQWYAPSQGRYLPDRYVEGTCYICGYASARGDQCDNC
                     GSLLEATQLLNPRSKIDGSTPELRETEHFYLDLHKLQPEIVDFLRQRESYFRPNVLRQ
                     SLGQILANDLHGRAITRDLDWGIPVPVDGWEGKCLYVWFEAVIGYLSATVEWSQVAGQ
                     PEAWHDWWYDPQARAYYFIGKDNIPFHAVIWPGQLIGAGEWFGKLFEGSEDNHLTLPF
                     DVPANEFMNLEGQKISGSRNWAVWGLDFLTRYDPDPLRYYLTANMPESKDTDWAWQDF
                     LNRNNNELVATWGNLANRVLAFAYKHWEGRVPEPSELRQPDRELLAQVEAGFQSVGEQ
                     LEAVRLRAGLMEVMRLATEVNKYLDQAAPWFEIKSDKNAAASSIYTALRAIDSLKLLF
                     CPFLPFTSQRLHTYLGYTQPLFGEQYVETYQDELGEHVALRYRPDKGSGRWEPSQLPA
                     GQVLQKPEPLFRKLDESILDEERQRLGKVDA"
     gene            complement(4853..5476)
                     /locus_tag="AC16CB_10605"
     CDS             complement(4853..5476)
                     /locus_tag="AC16CB_10605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3342962.1"
                     /translation="MKLFYEILYRRFRAPWDIGPRTELVELVESGRLEPGRAIDLGSG
                     TASNCIFLAQNGFTVTGVDYAESAIDLGIQRAQEAGVNVNFIKDDLTNLRYVTGTFDL
                     LVDYGTLDDLKPSARERYLQNVLPLTRPGSKFLIYCFEWPFRWWERLLFFLFSPISEH
                     LAVEPGEIEQRFGEHFEVEKFSGQVDYSQWPPGYAVYLMTRMDGHID"
     gene            complement(5473..6942)
                     /locus_tag="AC16CB_10610"
     CDS             complement(5473..6942)
                     /locus_tag="AC16CB_10610"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent decarboxylase
                     family protein"
                     /protein_id="HIR3342963.1"
                     /translation="MIGDKFSMDEETLDPQNWDAMRALGHRMVDDMLNYLQDIREHPV
                     WQPIPDNVKDKLRQPLPLEPQLPERVYQDFLEYVLPHPLGNIHPRFWGWVMGTGTPLG
                     MLAEMLAAGMNPNLWGTEHVANYVEAQVLDWCKAMMGFPKEASGLLVSGGSMANLIGL
                     AVARNAQAGFDIRRQGVSASPKPLVLYASMETHSSVQKAVELMGLGSQSLRKIAVNAD
                     FQIDLEALEKAIADDRAMGLNPICLVGNAGTVNTGAIDDLNSLANLAAREGMWFHVDG
                     AFGALAALSPELRPLVSGIERADSLAYDLHKWMSIPIEAGCVLVRDAEAHFNAFTLTA
                     AYLSQADRGLASGTMGYYNFGLQLTRGFRALKVWMSFKEHGIRKYGRVIQKNVEQARY
                     LANLVEEAPELELLAPVSLNIVCFRFKNDHLDDSQLDELNKELLIRLYESGIAVPSYT
                     TLRGKYALRVANVNHRSRNEDFDILVSEVIRLGKELMTS"
     gene            complement(7156..8337)
                     /locus_tag="AC16CB_10615"
     CDS             complement(7156..8337)
                     /locus_tag="AC16CB_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3342964.1"
                     /translation="MVYPSNASAGKQVLASLIEKANDQGYLTLDDILEADRHHGDEAT
                     IQPKKLLTYLRRQGVEIIEFDEAEINQAEPPDDLVVEFSLPTGFEKIPTEDTVEIYLK
                     EMARVPLLSVEEEIWLALRIEEGRAALSKLSQLKVNGNHRKRRALENTVQDGILAREH
                     IIAANTRLVVSIAKRYIGRGVPFLDLIQEGNLGLMKAVEKYDVHRGFRFSTYATWWIR
                     QTITRAIADQGRTIRLPVHMSDRIRHMYRAAHELEQSLGRFPTAEELAERMDVPARKV
                     QWMLQVSWIPLSLESPVGDEEDAELGMFVEDQNTPTPIQVVYQNTLRERVEEVLASLS
                     PREARVLRLRFGLNEDRPYTLEEVGEKFGLTRERIRQIEGKALRRLRHPICARRLRDY
                     L"
     gene            complement(8410..8925)
                     /locus_tag="AC16CB_10620"
     CDS             complement(8410..8925)
                     /locus_tag="AC16CB_10620"
                     /inference="COORDINATES: protein motif:HMM:NF014657.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR3342965.1"
                     /translation="MPNSTDQLRLNVGFLINQSIGSSREFLFDIPVFHLDSEFHLTNL
                     GGMAKFTRIAQGLLAQVKMQATLQTECVRCLTDFQQELKIDFTELYAFSPNSITDSEL
                     LVPENAQINLRPLVREYMLLDVPINPLCSQDCKGLCSICGQNLNESTCHHEVEAVDPR
                     LSVLKSLLEKK"
     gene            9065..9676
                     /gene="rdgB"
                     /locus_tag="AC16CB_10625"
     CDS             9065..9676
                     /gene="rdgB"
                     /locus_tag="AC16CB_10625"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HIR3342966.1"
                     /translation="MTKLLLASGNPGKLFEMADLLANLPIEILTPQQIELSIDVEESG
                     ATYAENAAIKAQAYARASGLLTLGDDSGLEVDALGGLPGIRSARFSPKPNATDAERRQ
                     ILLDQLMPHPRPWTARFRCTVAIATPHNELFFAQGECLGEIIPEERGENGFGYDPIFL
                     IVEYGLTMAELSTHTKNQISHRARAIQAARPILTKLITNTGET"
     gene            9709..11463
                     /locus_tag="AC16CB_10630"
     CDS             9709..11463
                     /locus_tag="AC16CB_10630"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HIR3342967.1"
                     /translation="MNADESSTFVDWINVSLRWLLLLALTVAAAINGNLPFEVILTLL
                     VAALWNVSLTLLATINRRLWAHRYMLLAGDVIIASLLFLFSGGLQGSLGWVGVLPLLT
                     SALYFRILGGLLGALGALLFQGLMALVVYQFMDVLAFLIPLLPIYLGVGILMGGLSGR
                     LTSLISQTQRDLISANKKSQRAERERNRAIYNLISALNASLNYQRVLETALDLGYSTL
                     AKNHESENRMVSSVLLYTKINGNDSELKVGAARRFTTADMRISLPGTTGLIGETIDKA
                     ESQLTSEVSNDPELSRIVAVRTCRSAYCIPLFTGLDTYGVLLFAHPDSNYFTSERCEI
                     LDIIGNQAIVAMQNARLYHELELEKERMMEIQEEARKKLARDLHDGPTQSVSALAMRV
                     NFTRRVMERDPGAAAEELYKIEELARRATKEIRHMLFTLRPLILESQGLVAALESMAD
                     KMRETYNQNVVIEADPEIVSQIDPNRQGVLFYIAEEAVNNARKHAQASNIWVRIKASA
                     EDFALLVIEDDGVGFDLVSVDSSYNNRDSLGMLNMRERTELINGKLKIEASEGKGTLI
                     QVLIPLDEDAAERIRLGQ"
     gene            11426..12232
                     /locus_tag="AC16CB_10635"
     CDS             11426..12232
                     /locus_tag="AC16CB_10635"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3342968.1"
                     /translation="MKMPPSASVSVSNTFYMPIADSIYYLVYQTGEKLPVVLLHSAGG
                     SLLSWPAEIRRLAGYRIYALDLPGHGKSGGQGYQSISSYADVVMEWLDAVGLSRAVFV
                     GHSMGSAIVQMLALDHPGHVLGLVLIGSAASLPVNPMLINESANQTTFNKAVEKVVSW
                     SFSPQTPQNIKTLVAKRLAETRPSVLHGDFLACDTFNVTNRIHEIHQPTLVICGSQDK
                     MTPVRNAYFLADNLPAARVEIIPGAGHMVMLERPQEVAASLAQFFRQIPY"
BASE COUNT         3078 a         3275 c         2877 g         3044 t
ORIGIN      
        1 cagcgcatta tcgctcctgc atgccatgga agctacactg gcaggttcat ttatatttaa
       61 acccagaagg gaatagtctt tatagttaat tcgataccag gctgattacc caggacaata
      121 tgacgatcac cgcgattaaa gcgaagataa tctgttgtgt gcgtactcgc cgtatttcac
      181 gcttggttgg acctttcttt ccctgtccca ttaataatct cctttaattt cattccaggt
      241 gttctttgat cgcctgcgca agcttggaag tgatccctga tacggcagtc aattcctcca
      301 ttgaggctgc ttgtatatcc tggatcgagc cgaagtgatt gagcaacaat ttccgccgtg
      361 ctggaccaat cccggggata gaatccaagc gggacgccag accttgtttg gtgcgcagtt
      421 tacggtgcga tgtgatggca aagcgatgtg cctcatcgcg cacgcgttga atcatatata
      481 aaccctggga ttgtctagat aaaataatcg gatcgcttct tcccggtaca aatagttcct
      541 cgttttgctt tgccaatcca gcgactggga ttttaccaag caatccaaaa cggtcgaaaa
      601 cagcggtggc gcgccctaat tggcctttgc cgccgtctat taagatcaga tctggcagcc
      661 ttgagaaaga tgtatccgga cgtttaccag gtttctggga ggcttcttga gcagcctgcc
      721 agcggttaaa acgacgggtg agtacctcct ccatactagc aaaatcatca ggaccggcca
      781 cgctgcgaat attgaaatgg cgatacagcg acttcttcgg cacacctcgc tcgaatacta
      841 ccatgctacc cacagcgttg gtaccctggg tgttggaaat atcataacac tcgatccgat
      901 tgggcggttc ggtcaggctt aatgccgatt gtaattcagc caaagcctgt tcctgccgca
      961 ctgtatcggc atgccactgt gcctggagcg ccgccagggt ttccgtggca ttttgcaatg
     1021 ccatttcaat taactcatgc tgcgccccct cgcgcggcac caggatttcc actttttgtc
     1081 cgctactctt ttgatttaac cattgggtga tgatctgggc ttcttccacc tcatggggta
     1141 ggagtacgtg ttgaggcaca ctgggtgcct gatcgtaaaa ctgcttgatg aattcagcca
     1201 tcacacccgc atcaggggtt tcttcagtac cctccagcag gaaatattcg cggccaatta
     1261 atttaccccc gcggataaaa aacacttgca cacaggcttc accatccgca cgggcaatgg
     1321 caatgacatc cgagtcgacg tactccgacg agatcactcg ctggcgagct actacatttt
     1381 ctatcgcccg caattggtcc cgaatcacag ccgcctgctc gaaacgcaac gcttcagagg
     1441 cccgctccat ctcagcctgc aaacgcgtca ccaccggctc ggtgcgaccc ataagaaaat
     1501 ggcaaaaatc gtcgatcata cgttggtaat ttgcctgatc aatggccccg atacatggtg
     1561 cggtgcacaa tttgatgtca tagtacaggc aagcgcgctc gtcatttcca gtgatgacgc
     1621 gatcacaggt caggtaaggg aatatacgcc ttaacacatc gagcgtctgg tgtactgccc
     1681 acacgctggt atatggccca taataccgcg atccatcctg gaccatctgg cgggtcacgg
     1741 tgatcttagg aaaagggttg ccccagtgaa ctttgatata aggataacgt ttatcgtcct
     1801 tcaggcgaac attgtagtgc gggcggtgct tcttgatcag gttcatctcc aagatcagcg
     1861 cctccagctc ggagtccacc acgatccatt cgatatcccg tatcttgcga accagtttta
     1921 ggatacgccg ctcatgttgg gcactgtcat gaaaatagga tcgcacccta ttgcgcaggt
     1981 taatagcctt gccgacatag atcaccttgc cggcttcatc tttcatcaga tagcagccag
     2041 gtttggtggg caaagtatcc aagatagcct gaagattgga ttgtaaatcc atcgccaaga
     2101 ttctaacata gtcaccccac agatatttat cccagcacat caattagaaa ctgcgcagtt
     2161 tgacacaata taggttaaac ccagattgct gttattcaac tgcccaatcc aaatataata
     2221 caaccatgga cagtatactg ctcgaactac agattggtga aatactcacc caacgcagat
     2281 taaccctggc tacagctgaa tcctgcacag gaggcctggt cagtcatcgc atcaccaatg
     2341 tgccgggctc atcccattac ttcttgggcg gcgtggtggc atacgcaaac cagacgaaga
     2401 tggcattgct ggatgtacgc ttggatacct tagaaaaatt tggcgctgtg agtgaagcaa
     2461 ccgtgctgga aatggcacgc ggagtgcgcc aggcactcaa tgcagatatc ggcgtcgctg
     2521 ttagcgggat cgctggccca ggcggcggca ctcctgaaaa accagttggg ctggtttgga
     2581 ttggaattag cggctggggc gaagaccaag ccaggagcta tgtttggtcg ggtgaccgca
     2641 tggcggttaa agaacattcc gcccaggccg cgttagaaat gctcttaagt atgctggata
     2701 aatagcctat tgcgatcaag cgtctacttt gccaagccgt tgccgctctt catccaaaat
     2761 gctctcatcc agcttgcgaa acagcggctc gggcttctgc aatacctgac cagcaggtag
     2821 ctggcttggc tcccagcgcc cgctgccttt gtcgggacgg tagcgcagcg ctacatgctc
     2881 accaagctca tcctggtagg tttccacgta ctgctccccg aaaagcggct gggtatagcc
     2941 caagtaggta tgtaaccgct ggctggtaaa tggaagaaag gggcagaaca acaatttgag
     3001 ggaatcaatt gcccgcaacg cagtgtagat tgaagaggct gccgcattct tgtccgattt
     3061 gatctcaaac cagggggcag cctggtccaa atacttgttc acctctgttg ctaagcgcat
     3121 aacctccatc agtccggctc gcaggcgcac cgcctccaat tgttcaccca cagattgaaa
     3181 gccagcctcc acctgggcta ggagttcgcg atcaggttgg cgcaattcgc tcggttctgg
     3241 cactcggcct tcccagtgct tgtaagcaaa ggcaagcact cggttcgcca ggttgcccca
     3301 ggtagcaacc agttcgttat tgttgcgatt gagaaaatct tgccaggccc aatctgtatc
     3361 cttgctctct ggcatgttgg cggttaggta atagcgcagt gggtcaggat cgtaacgggt
     3421 gaggaaatcc agcccccaca ctgcccaatt gcggctcccc gagatcttct gcccttctaa
     3481 gttcatgaac tcgttcgctg ggacatcgaa gggtaacgtc agatggttat cctccgaacc
     3541 ctcaaaaagc ttaccaaacc actcacctgc accgatcaac tgccctggcc aaattactgc
     3601 atggaaaggt atgttgtcct tgccaataaa gtagtatgca cgcgcctggg ggtcatacca
     3661 ccagtcatgc caagcttcag gctgaccagc aacttgcgac cactctactg ttgcggagag
     3721 atatccaatg accgcttcaa accatacata aaggcacttg ccttcccagc catccactgg
     3781 cactggtatt ccccaatcca aatcacgtgt gatagcgcgc ccgtgcaggt catttgccag
     3841 aatttgacct aatgattggc gcaacacatt aggtcggaag tagctctctc tttggcggag
     3901 gaaatcgacg atctctggct gtagcttgtg caaatcaaga tagaaatgct cagtctcgcg
     3961 cagctccggt gtgctgccat cgatttttga acgcggattt aataactggg tggcttctaa
     4021 tagactaccg caattatcgc attggtcacc gcgcgcacta gcatagccac aaatataaca
     4081 agtgccctcc acatatcgat caggaaggta gcgcccctgt gagggggcat accattgaag
     4141 ctgcctttct gtatacaaat atccattatt ttttaacgcc aggaagattg cctgagacac
     4201 tttgaagtga ttctctgtat gcgtgctggt gaaaagatcg taagccaaac cgagtttctg
     4261 aaaaagctct ataaagcctg cgtggtactt ttggtaaacc tgcaaagctg tggaattctc
     4321 cgcatcggca cgcactgaaa cgggggtacc gtgggcatca gagccagaga ccattaatac
     4381 gcgattaccc ctcaggcgct ggaagcgggc gaagatatct gccggaagat aggatccggt
     4441 gatattaccc acatggatga gcgagttggc gtaaggccaa gccaccgcca ccaggatatt
     4501 ttttgtcatc agtacctccc acatctaata aaaaggctgt ccttctcagg acagcctgtc
     4561 atatctcttt gagggccggc tacgccggat tcccactggc tgcctgaacg cgatatttca
     4621 tttccatgcg catcatcatg gacataacga aggtcatccc attaaccttg agcgataata
     4681 tacgcaccat ctcaagcaac ctgcagaaag atcttggtaa gtctatattg ggctacagga
     4741 tttgtcaagt gttcgcctcg tgtgttcgcc tcgtgttcac agcctcgttt gttgttataa
     4801 agtgaacact ctttaatcga agccagcagg gtgagtgata acatttttcc aatcagtcta
     4861 tatgcccatc cattctggtc attaaataaa cagcgtaacc aggaggccat tgcgaataat
     4921 caacctgacc agagaatttc tctacctcga aatgttcccc aaaacgctgc tcgatctcac
     4981 ccggctcgac cgccaagtgc tcagaaattg gagagaaaag gaagaacaac aagcgttccc
     5041 accatcggaa gggccactca aaacagtaaa taagaaattt gctgccggga cgcgtcaagg
     5101 gaagaacatt ctgcagatag cgctcgcgag ctgatggctt taggtcatca agcgttccat
     5161 agtcaactaa cagatcgaat gtgccggtta catagcgcag attagtcagg tcatccttga
     5221 tgaaattcac gttgacacca gcctcctgag cacgctgtat acccaaatcg attgctgact
     5281 cagcgtagtc cacgcctgtc acggtaaacc cattttgagc taaaaatatg caattgctgg
     5341 ctgtaccact accaaggtca atggctctgc ctggctcaag acgcccactt tctaccagtt
     5401 ccaccaattc agtgcgtggt ccaatatccc agggagcccg aaagcggcga tacaagattt
     5461 cataaaataa tttcacgatg tcatcaactc cttacccaaa cggataactt cgcttaccaa
     5521 gatatcgaag tcctcattac ggctgcgatg gttcacatta gcgacccgca aggcatattt
     5581 cccgcgcagc gtcgtatagc taggtactgc gattccactc tcataaagtc ggatcagcaa
     5641 ctctttgttc aactcatcga gttgagaatc atccaggtga tcatttttaa aacggaaaca
     5701 tacaatattt aacgacacag gagccaaaag ctccaattct ggcgcttctt ccaccaagtt
     5761 tgccaggtag cgtgcctgct ccacattctt ttggatcact cgcccgtact ttcgaatgcc
     5821 atgttcctta aaagacatcc atactttcag ggcgcggaat ccgcgtgtca gttgcaagcc
     5881 aaagttatag tatcccattg tccctgaggc aagtcctcta tccgcctgcg agaggtaagc
     5941 tgctgttagc gtaaacgcat taaaatgcgc ctccgcatcc cgtaccagaa cacatcccgc
     6001 ctcaatagga atagacatcc acttgtgcag atcataagct aatgaatcgg ctcgttcgat
     6061 ccccgaaacc agcgggcgta gttcaggaga gagagccgcc aacgcaccga atgcaccatc
     6121 cacatggaac cacatccctt cgcgcgcggc taagtttgcc aggctgttca ggtcatcaat
     6181 ggcacccgtg ttcaccgtac cagcgttgcc caccaaacaa attggattca aacccatcgc
     6241 tcggtcgtct gcaatcgcct tttctaatgc ttccaagtca atttgaaaat cagcattaac
     6301 cgctatcttg cgcagcgact ggctccctaa ccccatcagt tcgaccgctt tttgcactga
     6361 gctgtgcgtc tccattgagg cgtagagaac caatggtttt ggtgaggcgc tcactccctg
     6421 gcgacgaata tcgaagcctg cctgggcatt acgcgcgacc gcgagtccaa tcaggttagc
     6481 catcgaacca ccactaacca gcagtccgct tgcctccttg ggaaagccca tcatcgcttt
     6541 gcaccagtcg agaacctgcg cctcgacata gtttgccaca tgctctgtac cccacaggtt
     6601 tgggttcatc ccagccgcca gcatctcagc cagcatgccc agtggtgtac ccgtacccat
     6661 cacccaaccc caaaagcgcg ggtgaatatt acccaatggg tgagggagca cgtactcgag
     6721 aaaatcctgg taaacccgct ctggcaactg cggctccagt gggagcggtt gtctcagctt
     6781 atccttcaca ttgtctggga taggctgcca cacaggatgc tcacgaatgt cctgtaaata
     6841 gttaagcatg tcatccacca tgcgatgccc taatgcccgc attgcatccc aattttgagg
     6901 gtcaagcgtc tcctcatcca tagaaaattt atccccgatc atagatttcc tctttcagac
     6961 aatgcataaa ttccagctaa tcagatcagt tgtatcatat tataacaaaa tcattccagt
     7021 gtctggctcg ctttacttct ggcgaggctc catcaaatat ccccccaatc cttctaaacc
     7081 catctgaaca aacccacaat tacatcacag gtatcctctg ttagcaccaa gctattaaaa
     7141 gcagttcact tcgggctata gataatctct aagtcgccga gcacatatcg gatgacgcaa
     7201 tcgccgcagc gcttttcctt caatctggcg tattcgctca cgcgttaaac caaatttttc
     7261 gcccacttcc tccaaagtgt atggacgatc ttcattaaga ccgaagcgca agcggagcac
     7321 cctggcctcg cgcggcgaaa gcgaggctag cacttcttcc actctctccc ttagtgtatt
     7381 ctgatacacc acctggatcg gcgtgggtgt gttctgatct tcaacaaaca ttcctaactc
     7441 tgcatcttcc tcatcaccga ctgggctctc aagcgagagc ggaatccagg acacctgcag
     7501 catccactgt actttgcggg ccgggacgtc catccgctca gccagttcct cagctgtggg
     7561 gaagcgtcct aaagattgct ctaactcgtg agccgcgcgg tacatatgac gtatgcggtc
     7621 cgacatatgt acaggcaaac ggatggttcg cccctggtcg gcaatcgcgc gtgtgatggt
     7681 ttggcggatc caccatgtgg cgtaagttga aaagcggaaa ccgcgatgga catcatactt
     7741 ttctacagct ttcatcaagc ctaaattgcc ttcttggatt aaatctaaaa acggcacgcc
     7801 gcgacctata tacctcttcg caatgcttac caccaaacgg gtgttggcag ctatgatatg
     7861 ctcacgcgcc aagataccat cctgtactgt gttttccaag gcgcgacgtt ttcgatgatt
     7921 accgttaacc tttaattgtg aaagcttgct cagcgctgcg cgcccctctt caatgcgtaa
     7981 tgccagccaa atttcttctt ctacgctcaa gagcggcacg cgggccattt ccttcaaata
     8041 tatttcgacg gtgtcttcag taggtatttt ctcaaagcca gttggcagac tgaattcaac
     8101 aaccaaatca tcgggtggtt ccgcctggtt aatctcagcc tcgtcgaact cgatgatctc
     8161 aaccccttga cgccgcaagt atgtcagcaa ttttttcggc tgaatggtgg cttcgtcccc
     8221 atgatgccgg tcagcttcca agatatcatc cagggtgaga tacccctgat cattagcttt
     8281 ctcaattaaa cttgcaagca cctgtttccc tgctgatgcg ttggacggat agaccatgcg
     8341 cttccctcct aatacgacga gctttactgg ttagctctac tcaagtgagc cttattgtga
     8401 tgcaggggat cacttctttt caagcagtga tttcaataca cttaaacgag ggtcaacagc
     8461 ctcaacttca tggtgacatg tcgattcatt caaattttgc ccacaaatcg agcaaagtcc
     8521 cttacaatct tggctacata acgggttgat gggcacatct agcagcatgt attcccgtac
     8581 tagcggtctc aggttaattt gcgcgttttc aggaaccaaa agctcggaat ccgtgataga
     8641 gtttggcgag aatgcgtaca attcggtaaa atcaattttg agttcttgct ggaagtcagt
     8701 gagacaacgc acacactcgg tttgaagcgt agcttgcatt ttgacttgtg ccagcaatcc
     8761 ctgtgcaatg cgtgtgaatt tcgccatacc ccccaggttg gtcagatgga attccgaatc
     8821 cagatgaaat actggaatat caaaaagaaa ttcccggctg gagccgatag attgattaat
     8881 aagaaaaccg acattgagcc ggagttggtc agtcgagttt ggcactgatg ctggaatgaa
     8941 tgatataaaa ttatgctatt aattttgaaa aattatagca tagaattgat taatagtcaa
     9001 gatttgctta ttattttgac aaaatcaaat tgcctgtaaa aatttattgc aatcggagat
     9061 caagttgact aaattacttt tagcgagcgg caatccggga aaactttttg agatggcaga
     9121 tctgctggct aacttgccca ttgaaatcct gaccccccaa cagatcgagc tgagcataga
     9181 tgttgaagag agtggagcaa cctacgcaga aaacgcggca atcaaagccc aagcctacgc
     9241 ccgcgcctcc ggattgttaa ctttggggga cgattccgga ttagaagttg atgccctggg
     9301 aggattacca ggtatacgtt cagcccgctt ttcccccaaa ccgaatgcga ccgacgccga
     9361 gcgtcggcaa atccttcttg atcagcttat gccgcatcca cgtccctgga ccgcgcgctt
     9421 tcgctgcacg gtagcgatag ccactccaca taacgagcta ttttttgctc aaggggagtg
     9481 cttgggcgag attatcccag aagagcgagg cgagaacggt tttggctatg acccgatctt
     9541 cttaatagtg gagtatggct tgacgatggc cgagttaagc acccacacaa aaaatcaaat
     9601 cagtcatcgt gctcgggcaa ttcaggccgc gcgaccaata ctcacaaaac tgataacaaa
     9661 tactggtgag acttaacctg ccattgtttt tcgattatga taacccctat gaacgccgac
     9721 gagtcatcga cttttgtcga ctggatcaat gtctcattgc gctggttatt attgctggca
     9781 ttaacggtag cagctgccat caatggcaac cttccattcg aagttattct gactttattg
     9841 gttgcagcgc tttggaatgt ctccctaaca ctcctggcaa ccatcaaccg gagattatgg
     9901 gcacaccgtt atatgctcct cgctggtgat gtgatcatag ccagcctgct gtttctattt
     9961 agtggtggat tgcagggtag tctgggttgg gtaggtgtgc tccccctgct tacatcggca
    10021 ctgtactttc ggattcttgg agggctgctg ggagcgctag gagcgctgct ctttcaaggt
    10081 ttgatggcct tggtggttta ccaatttatg gatgtgttgg cattcttaat ccccctcctc
    10141 cctatttatc ttggggtggg gatcttaatg gggggcttgt ccggacgctt gactagccta
    10201 atcagccaaa cacaacgcga cctaatctca gctaacaaaa aatcccagcg ggccgaacgt
    10261 gaacgaaatc gggcaattta caacctcatc tccgcactca atgcctcact taattaccag
    10321 cgtgtcctgg aaactgccct tgatttggga tattctaccc tggccaaaaa ccacgaatcc
    10381 gagaatcgaa tggtcagcag tgtattactt tatacaaaga taaatggaaa tgattcagag
    10441 ctgaaagtgg gcgcagccag gcgcttcacc acggcagata tgcgcatctc cctacctggg
    10501 acgaccggct tgattggcga gacaattgac aaggcagagt cgcaattgac cagcgaggtc
    10561 agcaatgatc cggaattgag ccgcatcgtg gcagttcgta cctgccggtc tgcctactgc
    10621 attcctctct ttaccgggct cgatacatat ggtgtgctcc ttttcgccca cccagatagc
    10681 aactacttca ccagcgagcg ctgtgagata ctggatatca tcggcaacca agctatagta
    10741 gctatgcaga atgcccgctt gtatcatgaa ctagaacttg agaaagagcg tatgatggaa
    10801 atccaggaag aggcccgcaa gaagctcgcc cgcgatctgc atgatggccc aacgcaatcc
    10861 gtatccgccc tggcgatgcg cgtaaacttc acccgccgcg tgatggagcg cgatccaggc
    10921 gcagcggccg aagagcttta taagatagaa gaactggcca ggcgtgctac caaagaaatt
    10981 cggcacatgc ttttcacgct acgcccgctt atcctcgaat cccaggggtt ggtagcagcc
    11041 ctggaatcta tggcggataa gatgcgcgaa acctacaacc aaaatgtggt aatcgaagct
    11101 gacccagaga ttgtctctca aattgacccg aacaggcagg gcgtgctctt ttatattgcg
    11161 gaagaagcgg ttaataacgc ccgcaaacac gcccaagcct cgaacatctg ggttcgaata
    11221 aaagcatccg cagaagattt cgcgctgctt gttattgaag atgatggagt gggtttcgat
    11281 ctcgtatctg ttgattcatc ctacaataac cgcgacagtc tcggtatgtt gaacatgcgc
    11341 gagcgcaccg aattaatcaa cggcaaactt aagattgaag cgtctgaagg aaaaggcact
    11401 ctaatccagg tgctcatacc tctcgatgaa gatgccgccg agcgcatccg tctcggtcag
    11461 taatactttt tatatgccca tcgctgatag catctactat cttgtctatc aaacaggaga
    11521 aaaactacca gttgtcctgc tgcacagtgc aggcgggtcg ctcctttctt ggcctgcgga
    11581 gatccgccgt ctggctggat atcgcatcta cgccctagac ctgcctggtc atgggaaatc
    11641 gggcgggcag ggatatcaat ccatctccag ctatgctgat gttgttatgg aatggttgga
    11701 tgcagtgggc ttatctcgcg cggtcttcgt tgggcattct atgggcagcg ccattgtcca
    11761 gatgttggca ttagaccatc ctggccatgt gcttggatta gtattgatcg gcagcgcagc
    11821 cagtttgccc gtcaacccaa tgttaatcaa tgagtctgct aaccaaacca ccttcaataa
    11881 ggcggtggaa aaagttgttt cctggtcttt cagcccccaa acgccacaaa acatcaaaac
    11941 cttggtagcc aaacgtttgg ctgaaacccg cccaagcgtg ctgcatggtg atttccttgc
    12001 gtgtgacacc ttcaacgtga ccaaccgtat tcacgaaatt catcagccca cattggtgat
    12061 ttgcggctcg caggacaaaa tgacccccgt gcgaaacgcg tacttccttg cagacaacct
    12121 tcctgcggct cgcgtggaaa tcataccagg ggctggacac atggtcatgt tagagaggcc
    12181 tcaagaagtt gctgcctccc tggcgcaatt ttttcgccag atcccatact aattctcaaa
    12241 ctgagagcat tttcgcttga cgcccatggg tggg
//
LOCUS       DBNGSX010000355        12175 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_307399, whole genome shotgun sequence.
ACCESSION   DBNGSX010000355 DBNGSX010000000
VERSION     DBNGSX010000355.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12175)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12175)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12175
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_307399"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="AC16CB_10640"
     CDS             complement(<1..1333)
                     /locus_tag="AC16CB_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3342969.1"
                     /translation="MTSSQNLPSSIHPMSRHLLVRVVQAAIQLGHTRFARQACLNWLA
                     AYPGDLPVNLLYANVLLQDGRNEQAIPILARLCQVDPEYLDAVQAYLRAQKGLHTPGE
                     AQSKNARRRRQRLQNSTNDILNWAMALGINPRAEKATTLPTSSQEASTPIWSEQVLQA
                     RRALEASIASDDKNHELDEAEIALTPALANSSASPLVGVTQLRVLKAKNNPTQSIRGL
                     AEYYLKQWPECVQFQLFLAETLMETGQEEEAVALLHRAAARDLTGQVAIRLWGRGHPY
                     RSLWPERLEHALDIPIPAQVAAALGWNQLPVGEPITTPLKRDEALPVNHPVPKGIEKL
                     PLTNENHDPSETGATEPAGSPKPIFPTIPETLRSVQVELEKVAQRLQQPELARADGRF
                     PVYVIMTTQNGLHAQYGEKASVLTAEMQRLAQAVGARRDWRSVLFFADEGLP"
     gene            complement(1368..6968)
                     /locus_tag="AC16CB_10645"
     CDS             complement(1368..6968)
                     /locus_tag="AC16CB_10645"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3342970.1"
                     /translation="MTIASFTSSLKALLTESNWPWVFSALSLDPLVWESLDGELGLQA
                     IERQSNQPEDYSPAALALLALGITASAESLRNANGEEIQPAIDPEVNPTSPLAQSGLA
                     ALQLREARKTSASWDALFRLKGINTPTTLACLYTIIPDQADMLRAAYSLEASDKSRPS
                     INPTLQALLSNPLAPAVHLEALNELVSDLPRSERLELLGQLQRYRPDLAPPIAQVTLN
                     LEPSFLGTPSNGNGDGLRGLAQIEKSFRAARLHMLAQSTEQALDCLNEATLAARQVQA
                     KVSAQIAQIIAQTNDSSAQIAAWEEAVQLDPGSADHITGLILALLDNERFEEAEKILA
                     QEELDVKHSGILFANARLAMMQGNSELARQYAIQATTSMNNNSGSVIAAVLTGPELAS
                     MLLELDAPSEAARAAEIALANRPNDPQLLALLARANLAAHRHAQAVQAAHLATALLPD
                     QLDFRRLLAECLELTGDWSAALQERLILLDRTESSTAPELRALANCALHAGQSGRTAH
                     ICQLLIKEDKNDGLAWSMLGQATAELGDTTAALDHLQHATQLTPELSAPWLALAQLYE
                     NMGQPQKALETLQASALTAPDQPEIHLALAEAYLAENASTQALSSLRMASSLVAEKSV
                     GDTSPDYWVGTSKIKAGNRSLADQIALRLGQTLHQLGHHDEGRQTLEEAYSRSPNDID
                     VAQAYARVLLALGNVSAAIEPLETVVKAGPSNVTPYLDYARSVLELQSEHGNGAAIEN
                     AISAIRTAQQLEPDNLEVPALLAFALAANNELPPAMEAYRQALESYLAQDPHWLARLS
                     LGLGKAAHQLGQIETAIAALQEASQADPSNSQIQRSLSEAYDSAGLVEDAYQAARAAL
                     LLAPGDVENLNWFANQVLNLKGRPGGEFPDAHNEAIESLERATQIAPQRGDLWLRLGG
                     LQLQSGNRAGAVDALMYLADPESPVSESSANNLYQAAQWLNELGKADSAVICLERALQ
                     SGLEAHTPAGPSLLDMLTSLCAAHRQVGDLPAALQALDQAIAINPDEPELYLQKADIL
                     LGMSQTDGDANDSQAFAEQGVACLEAAMKLNPHNPDLHYRAALTHRAAGELHLALVHA
                     TEMVDISASLSQAMRARSLAADLSLAMLQPVQARTFLEANLPPSPPNEMVEDNVLLDY
                     YTMRAELALEAGDERGAVEELVHILEVAPDDPRLLAIQARVILQRDDPQAASSMLENV
                     LKGMGDPSKARLALLRSAAQAALELGQWEVAIQLCQQAISTAPLEPRSHLDLGRTLVL
                     RAEFSTMCQALGAIGRAGDQQAFSDEIQPIVETALQKTEEQIKAWEAEGSIKLANSQN
                     GETSGRIKIKWVDPEAQIHRWRTRGQAIFQPGSEAVKALAALPPTADDAAARIACLRE
                     MGDLTTAGLVARDYPQHPLVLLQLALTLLDEKPRQAMAAIHAAADSLSQATANRRFRS
                     AFSLKEISPLVYALLANLFHRDGNRVSDRDNALQAILNALSIWPDEPRWHVLAAEIYL
                     AGDPMDSQGDLEAAINHLERATKLDPEYTPSLIVLGQMYLREGSIQRAMQTFEQAAQA
                     APGEAEPWMWLAQACRSMGKMEQAAAHAERAVTLAPNYIKPLILRGEIALQDENPRGA
                     LSRAEAALRIQPDDPDALLLQVRALNALDRYEEALGTLEKALSLASDPLPLSMERVRL
                     LRRAQGNEAALHAIQSLADHYPDEPRVMTLQAEVLEAAGDSEAAIRAAQRALRGQAGA
                     PVISKVDQASLHFMLGSLLRSTGQLDQSLHHLSESIHLAPDQVNGYLEMGHVHLERRE
                     HTQALSAYRQAITVSPGDYRAYYSIGLALKESRDYVGAEKMLRRAAELAPDDVSIHRL
                     LGAVVALNLVHNRREPTREASRPM"
     gene            complement(6986..7309)
                     /locus_tag="AC16CB_10650"
     CDS             complement(6986..7309)
                     /locus_tag="AC16CB_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342971.1"
                     /translation="MSPSLTFELLFVMVIILFFLGMASTITGIVILTTRATGRDIQTI
                     ATQTTRLAQKGLAEEVAGLVGNASSLLDAMNQLIRTAAGVGFFLTVFGLLLMVAAAWL
                     ALQIY"
     gene            complement(7560..8480)
                     /locus_tag="AC16CB_10655"
     CDS             complement(7560..8480)
                     /locus_tag="AC16CB_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342972.1"
                     /translation="METTATMSLKRPLLFFALFCGFLLAGLSVGRQIVSWRARNLPQL
                     LARSNQLEKQSLPRENTPDLAAQTDHQVSQPPPLPAIPPSQAPSNAIIENGEPLPRQN
                     NILLIGVNNLDVAYPRLESTWLILYMTQMPHLTLMPIYPENPSQDENAEPPGTDLAKL
                     FQSDPKGLPGPDFFTELKKRGLWWSGYIIVDRQALNEVIDLVGRLAEQSGEGELSPEM
                     GILESVPSAWNNPQRAYEGQIQVAQRLCSAAAHITVDHINEIDDIFLLFTDDILSDIN
                     REGAAAELVGMLVQGSGITCEFPTLGNESP"
     gene            complement(8459..9613)
                     /locus_tag="AC16CB_10660"
     CDS             complement(8459..9613)
                     /locus_tag="AC16CB_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342973.1"
                     /translation="MTYKIKPLLLKITLVSLFIGVLASYKVGVSEPARAARGLPNSPE
                     FGYGARLDVWGQEVELALNAAAGVGIDWIGVDFDWARHWPDANHPINLEPLDKALAIA
                     QKQDLNVLLSISHPPAWVLGTDGPDVNQTAGLVVMLANRYPQNLMAIELFPQANTVAG
                     WGASPNPGAYSQLLLASQKALNAGARPVTLVAAGLSPLPAQPAVGDMDDLVYLSELYQ
                     AGAAANMPILSLRLSTVVGEAMASPNQDTRQVLRHYEAVRQVMLQHDHAHGLIWVTGF
                     SWPEESLDSPSDQQIRWLNQAFQLMKSQLYIGVAFFESLNPPQDDKPSASPTQSLIMN
                     DAQGTHMHPALVALGQIITLNQSGHASFQVFLYKKITTGPAKSLWKRRQP"
     gene            complement(9683..9895)
                     /locus_tag="AC16CB_10665"
     CDS             complement(9683..9895)
                     /locus_tag="AC16CB_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342974.1"
                     /translation="MSIRTITSRIEIAFWSVVVSLLSDSELIQGFVQTCYIGFTSGQY
                     TRTLKKALILSAAGFLIGIVLGLAGL"
     gene            complement(9925..10179)
                     /locus_tag="AC16CB_10670"
     CDS             complement(9925..10179)
                     /locus_tag="AC16CB_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342975.1"
                     /translation="MDMHTLRAVIAQIHKRYPEFAGCNPKVRLQNAPQPKSLLITPTY
                     LLTFQGTARANSATGEKAIPRWMRVVISEKGKILKVTTSR"
     gene            complement(10184..11386)
                     /gene="ftsZ"
                     /locus_tag="AC16CB_10675"
     CDS             complement(10184..11386)
                     /gene="ftsZ"
                     /locus_tag="AC16CB_10675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR3342976.1"
                     /translation="MSTLPYPSEFESQFADIRKPVLKVIGLGGGGGNAVTRMMELGLK
                     DVEFIVANTDHQVLEKNPAPVKIQLGPRVTRGLGAGGNPSIGRAATEESCREIASALQ
                     GADMVFLTAGMGGGTGTGAIPVVAEIARSLGAVTIAIVTTPFSFEMGRRQRNASEGLD
                     LLRKYTNTLIAIPNDRLLFIAPRNLPIETAFRLADDVLRQAVQGITELITEPGMINVD
                     FAHVRRLIQLGGGAMMSIGQGEGEHKTRKALEQALHHPLLETIALENAAGIIANFTGG
                     DDLTLFEVQDALANLQEQTSNRTEIVLGVIQDERMEDRVQITLIVTGLGAPTLEETLF
                     KVAAPSKAEAPKVPQQAALASDPKPMPIPTEKTEPIPQVTSGISPSNLDLPAFLRRRA
                     RLAGLENG"
     gene            complement(11412..>12175)
                     /locus_tag="AC16CB_10680"
     CDS             complement(11412..>12175)
                     /locus_tag="AC16CB_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342977.1"
                     /translation="GFHIFDWYLAEAQAALGKPMPIFLLAAGRRLHNTDESPATEIDE
                     LEHANINLSIAKQFMAGASSDSISEPISPFVLACNFWLLTADPSEPSANQAWFQPEDY
                     HLPIVAALHKLVRPGDAQQPDQSTPAHQLESKPDLHPPSSTLEDTHPSPVEPKIISAQ
                     PQDEHPIAHYLLLPKYDWGASEWHLDAARPFILKHNPTVGYSPAEAALAARITVVGGE
                     QVFPEELIDEMRQRGCQVTRITGDGTEIASKLAST"
BASE COUNT         2597 a         3178 c         3461 g         2939 t
ORIGIN      
        1 gtggcagacc ttcatcggca aaaaagagca cagaccgcca gtctcgacgc gcccccaccg
       61 cctgggctag tcgctgcatt tcagctgtaa ggacagaagc cttttctccg tattgggcgt
      121 gcaatccatt ctgcgtggtc atgatcacgt atactggaaa gcgtccatca gcgcgcgcca
      181 gctcaggctg ttgtagcctc tgagccactt tctccagctc tacctgaaca gatcttaaag
      241 tctctggaat ggtaggaaaa attggttttg gacttcctgc aggctctgta gcgcccgtct
      301 cagaagggtc atggttttca ttcgttaatg gtagtttttc tatgcctttg gggactgggt
      361 ggttaactgg aagcgcttcg tctctcttta gaggggtggt gattggttcg cccacaggca
      421 gttggttcca gcctaacgcg gcggctacct gggcagggat aggtatatcc agcgcatgct
      481 ccaaccgctc aggccacaaa gaccggtatg ggtgacctct tccccacagg cgaatggcta
      541 cctgacccgt cagatcccgc gcagccgctc gatgcaagag cgcgactgcc tcctcctcct
      601 ggcctgtttc catcaaagtc tctgccagga ataattgaaa ttgcacgcac tctggccatt
      661 gctttaggta atattcggca agaccacgaa tgctttgcgt cgggttgttt ttggctttta
      721 ataccctcaa ttgtgtgacg cccaccaaag gggaggctga gctgtttgca agagcagggg
      781 tcagggcgat ctctgcctca tccagctcat ggttcttatc gtcgctggca atgcttgcct
      841 ctaaagcccg tcgtgcctgg aggacttgct cgctccagat cggcgtgctg gcttcttggg
      901 aagaggtggg cagtgttgtt gctttctctg ccctaggatt gatgcccaaa gccattgccc
      961 agttcaagat atcattggtg gagttttgta acctttggcg ccgccgtcgc gcatttttgg
     1021 attgtgcctc tcccggggta tgcagcccct tttgggctct caagtatgcc tgtacggcat
     1081 ccagatattc tggatcgacc tggcacaacc gcgctaaaat cggtatggct tgctcgttac
     1141 gaccatcttg tagcaggaca ttggcgtaca gcaaattgac gggcagatcc cctgggtaag
     1201 ccgccagcca gtttagacaa gcttgccgtg caaaacgtgt gtgccctagc tgaattgcag
     1261 cctggaccac cctgaccaac aggtggcgtg acatgggatg aatagaagaa ggtagatttt
     1321 gcgatgatgt catcggtgta tttcaaccaa catctaaata ggctggctta cattggacgg
     1381 ctggcttcac gggttggctc acggcgattg tgtaccagat tgagcgctac gacagcccct
     1441 aatagacgat gtatgctgac atcgtcgggc gccagctctg ctgcgcggcg cagcattttt
     1501 tctgcaccaa cgtaatccct gctctctttg agcgccagac caattgagta gtaggcccga
     1561 tagtctcctg gtgacacagt gattgcctgc cggtaggcgc ttaatgcttg ggtgtgctcg
     1621 cggcgctcca ggtggacgtg ccccatctcc aggtagccgt ttacttgatc gggtgctaaa
     1681 tggattgact cgctcagatg gtgcaacgac tggtcaagct gcccagtgct gcgcaacagg
     1741 ctgccgagca tgaagtgcaa acttgcttga tcgacctttg agatcactgg tgctccagct
     1801 tggccgcgca gcgcgcgttg agctgcgcga atagcagctt cactgtcacc cgcggcttcc
     1861 agcacctccg cttgcagcgt catcacgcgg ggttcatcgg gatagtgatc ggccaaagat
     1921 tggatggcgt ggagggcagc ctcgtttcct tgggcacgtc gaagcagcct tactctttcc
     1981 atggagaggg gcagcggatc ggaagccaga gaaagagctt tctcaagtgt tccgagggct
     2041 tcctcatacc gatccagggc atttaaggcg cgcacttgta gcaggagcgc gtcgggatca
     2101 tctggttgga tacgtagagc ggcctcggcc cggctcagtg ccccgcgtgg attttcatct
     2161 tgcagggcga tctcgccgcg tagaatcagg ggcttgatat agttgggagc cagtgtgaca
     2221 gcccgttcgg cgtgtgcagc agcttgttcc attttgccca tactacggca ggcttgagca
     2281 agccacatcc atggctcggc ttcacccgga gcagcctgtg cagcctgttc gaaagtttgc
     2341 attgcccttt gtattgagcc ttcccttagg tacatttgcc cgagcacgat taacgagggg
     2401 gtatattctg gatcgagctt tgtggcacgc tccaagtggt tgatcgccgc ttccagatca
     2461 ccctggctat ccattggatc acctgccaaa tagatctcgg cggctaacac atgccagcga
     2521 ggctcatccg gccatattga aagtgcattc agtatggctt gtaaggcgtt atctcgatcg
     2581 ctgacgcggt tgccatcacg gtggaaaagg ttggctagca gggcatagac gagtggggag
     2641 atctccttaa ggctaaaagc agatcggaat cgacggtttg cggtagcctg agacagtgag
     2701 tcggcagctg catggatggc agccatcgcc tggcgcggtt tttcgtccaa taatgtgagg
     2761 gctaattgca gtagcaccag gggatgctgg ggataatctc gagccaccaa cccagcggtg
     2821 gtgaggtcac ccatctcccg caagcaggct atgcgcgcag cagcatcatc agcggtgggt
     2881 ggtaaggctg ccaaggcttt cacggcttcg ctgccaggct ggaagattgc ctgcccacgc
     2941 gtccgccagc gatggatttg cgcctcggga tctacccatt taatttttat ccggccggac
     3001 gtctcgccat tttgggaatt tgccaatttt atgctgcctt cagcctccca ggctttgatc
     3061 tgttcctcgg tcttttgtag agccgtctca acgataggct gaatttcatc cgaaaatgct
     3121 tgctggtccc ctgcgcgccc tatggctcca agtgcttggc acattgtgga gaattctgcc
     3181 cgaagcacca aagtgcgacc gaggtcaagg tgcgaacgag gctccaatgg tgcagtgctg
     3241 atcgcttgct ggcaaagctg gatagccact tcccattgac ccagctcaag cgcagcttgt
     3301 gctgccgagc gaagcaaggc taagcgggct tttgaagggt cacccatgcc tttaagtaca
     3361 ttctctaaca tgctcgaagc agcctgagga tcatcgcgct ggaggatgac gcgtgcctga
     3421 atagccagca gacgcggatc gtcgggagca acctcgagta tatgtaccag ctcctcaacg
     3481 gcaccccgct cgtcacctgc ttccagagcc aactcggcgc gcatcgtata gtaatcgagt
     3541 agcacattat cttcaaccat ctcattcgga gggctgggcg gcaaattcgc ctctaaaaat
     3601 gtgcgcgcct gaacaggctg cagcatagcc agtgagagat cggccgcgag tgagcgagct
     3661 cgcatggcct gggataggga agcactgata tcgaccatct cggttgcgtg caccagagcc
     3721 aaatgtaact ctcccgcagc ccggtgtgtc agagcggcgc gatagtgtag atcgggatta
     3781 tggggattta gtttcatggc agcttcgaga caggcaaccc cttgctctgc gaatgcttga
     3841 ctatcatttg cgtcgccatc agtctggctc atacccagta gaatgtctgc cttttgcaag
     3901 tacagctctg gctcatccgg attgatggca atggcctgat ccagagcctg taatgccgcc
     3961 ggcaaatcgc caacctggcg gtgagccgca caaagagatg tgagcatatc gagcaagcta
     4021 ggtccagctg gcgtatgagc ctccaagccg ctttgaaggg cgcgctccaa acagatcact
     4081 gccgaatctg ccttgcccag ctcatttaac cattgcgcag cctggtagag gttatttgca
     4141 gagctttcag agacggggct ttctgggtca gccaaataca ttaaggcgtc cacagcaccc
     4201 gcgcgattgc cagattggag ttgcaagcct cccaatctca accacaagtc gccccgctga
     4261 ggagcgattt gggtagctcg ctctagcgac tcaatagctt cattatgggc atccgggaat
     4321 tcaccccctg gtcgcccttt cagattcagt acctggttag caaaccagtt gaggttctcg
     4381 acatcgcctg gggcaagcaa caaggcagcc cgtgccgcct gatacgcatc ttccaccagt
     4441 ccagcggaat cgtatgcctc tgacaggctg cgctggatct gggaattgga tggatcggcc
     4501 tggctggctt cctgaagcgc tgcgatcgcg gtctcgattt gaccaagctg gtgcgcagct
     4561 ttacctaatc ccaaggaaag gcgagccagc caatgtggat cctgtgcaag atagctttcc
     4621 agcgcttgcc ggtatgcctc catagcggga ggcagctcat tgttagctgc tagtgcgaag
     4681 gcgagcagtg cggggacttc gagattatcc ggctcgagct gctgagcggt gcggatagcg
     4741 gatatggcat tctcaatggc ggcaccattt ccatgctcag attgcagctc aagcacactg
     4801 cgggcgtaat ctaggtaggg tgtgacgttg gatgggccag ctttcaccac ggtctccaac
     4861 ggttcgattg ctgcgctcac gttcccaagg gcgagcaaga cgcgtgcata agcttgcgca
     4921 acatcgatgt cgttgggcga acggctgtag gcctcctcca gagtttggcg cccttcgtca
     4981 tgatggccta gttggtgcag ggtttgtcct aaccgcaatg cgatctggtc tgccaggctg
     5041 cggttaccgg ctttgatttt agacgtaccc acccagtagt ctggtgatgt gtcaccaacc
     5101 gatttttcag ctaccaggct ggatgccatt cgcagcgagg agagtgcctg agtgctggca
     5161 ttctctgcca aataggcctc tgccagtgcc agatgtatct ccggttgatc aggagcggtt
     5221 agtgcagagg cttgtaatgt ttctagagcc ttttggggtt gacccatatt ctcgtaaagt
     5281 tgtgcgagtg ccagccaggg ggcagagagc tctggggtga gctgtgtggc atgttgcagg
     5341 tgatccaggg cagcagtggt atcgccaagt tcggcggttg cctgtccgag catagaccag
     5401 gctaaaccat cgttcttatc ctcttttatt agtagctggc aaatgtgagc cgtgcgccct
     5461 gattgaccgg cgtggagggc acagtttgca agagcgcgta attcgggggc ggtcgacgac
     5521 tctgttcgat caagcaggat caatcgttct tgtagggctg cagaccaatc tcctgtgagt
     5581 tccaggcact cggctagtag gcgacggaaa tccagctggt ctggtaatag ggcagttgcc
     5641 agatgggcag cttgcacggc ctgtgcatgc cgatgggcag ctagattggc acgcgccagt
     5701 agtgctaata attgcggatc atttggcctg ttcgccaaag caatctcggc tgctcgtgca
     5761 gcttccgatg gggcatctaa ttcaaggagc atgctggcta actcagggcc agtcagaaca
     5821 gccgcaatta ccgaacccga attattattc atggaagttg tggcctggat tgcatactgg
     5881 cgggcaagct cactgtttcc ctgcatcatt gccaaccggg cattggcgaa aagaatgccg
     5941 ctatgcttta catctagttc ttcttgggcg aggattttct cggcctcctc aaagcgctcg
     6001 ttatcgagca atgccagaat caatccagtg atgtgatcag ctgagcctgg gtctaattgc
     6061 acggcctctt cccaggcggc aatctgggca gacgagtcat tggtctgggc aataatttgg
     6121 gcaatttgcg ccgaaacttt agcttgtact tgccgggcag ctaacgtcgc ctcgttcagg
     6181 cagtcgagtg cctgttcagt agattgagcc agcatgtgca acctggcagc acggaaggat
     6241 ttctctatct gtgccaagcc tctcaatcca tctccgttac cattggaggg cgtgccgagg
     6301 aatgaaggtt ccaggttgag agtcacctga gcgatcggcg gagccaggtc cgggcggtaa
     6361 cgctgcaact gtcccaacaa ctccagccgc tcgctgcgag gcagatcaga aaccagctca
     6421 ttgagcgctt ccaggtgcac agcaggcgcg agaggattgg aaagcagtgc ttgtagggtg
     6481 gggttgattg atggtcgaga tttatcgctg gcttcgagcg agtaagcagc gcgcagcata
     6541 tcagcttgat ctgggatgat cgtgtacaag caggccagtg tggtgggagt gtttatccct
     6601 ttcaagcgga acagtgcgtc ccaagacgcg ctggtttttc gggcttcgcg cagctgcaat
     6661 gcagctaatc ctgactgtgc caagggagaa gtaggattaa cctctggatc gattgctggt
     6721 tggatctcct ctccatttgc gttacgcaag gattcggcac tggctgttat tcccagagcg
     6781 agcagagcca gggcagccgg tgagtaatct tctggctggt tggattgacg ctcgatagct
     6841 tgcaagccca actcgccgtc caggctttcc caaaccagtg gatccaggct taaggcacta
     6901 aagacccagg gccagttgga ctctgtgaga agagccttga gagatgatgt aaacgaggcg
     6961 atggtcatag atgcttcatc ctaatttagt aaatttgaag cgccaaccat gcagcagcta
     7021 ccatgagcag taatccaaaa accgttaaga agaaacccac tccggccgcc gtgcggatca
     7081 actggttcat ggcatccagc agcgagctgg cattaccgac tagcccggcg acttcctcag
     7141 ccagtccttt ttgcgccaat cgcgtggttt gggtggctat agtttggatg tcacgacctg
     7201 tggctcgtgt cgtcaggatc acaatgcctg tgatggtgga cgccataccc aggaaaaaca
     7261 ggatgatgac cataacaaag aggagttcga aggtgaggct aggagacata gcgacctcgc
     7321 agaaaaacat gaaatttgat ttacctgccg attaagatgc aagaaccgtg ccgagctact
     7381 gatttagaag ttacgcagtt ggatacgtgg cagggggggt tgggcgggcg tcgcccaccc
     7441 aaacccccct cctttcctct tcaactgcgt aggcctatga ttattagggc ggtcggaatt
     7501 gccacgtata aaaatcccta caccgtaccc ttttgttttt tactgcatta gctcgatgat
     7561 tatgggcttt cgttacccag tgtgggaaac tcacaggtga tcccgctgcc ctgcaccagc
     7621 atgccaacca gctccgcagc tgccccctcc cggttaatat cactcaagat atcgtctgta
     7681 aacaagagga agatatcatc gatttcgtta atgtggtcca cagtgatgtg tgcagctgcg
     7741 ctgcaaagcc gctgggcaac ttgaatctgt ccttcatagg cccgctgcgg attattccag
     7801 gcggagggta cgctttccaa aattcccatc tcaggagaga gttcaccctc tccgctctgc
     7861 tcggccagcc tgccaaccag atcgataacc tcgttcagag cctgtcgatc gacgataata
     7921 taaccagacc accacagtcc cctttttttg agctcggtga agaaatccgg accgggtaaa
     7981 cccttgggat cagattggaa aagtttggca agatcggtgc ctggcggttc agcgttctca
     8041 tcttgggagg gattttccgg atagatcggc attaaagtca ggtgaggcat ctgggtcata
     8101 taaaggatta gccaggtgct ttctaggcgt ggatatgcaa cgtccaagtt gtttacgccg
     8161 attagcagga tattgttctg gcgtggaagc ggttcgccgt tctcgatgat ggcgtttgag
     8221 ggtgcctgcg aaggggggat ggcagggagc ggagggggtt gagatacttg atgatcggtc
     8281 tgcgctgcga ggtctggcgt attctccctc ggcaaggatt gtttttctaa ctgattagaa
     8341 cgagctagga gctgtggtag attcctcgct ctccaagaca cgatttgcct gcctaccgac
     8401 agaccagcca gaaggaatcc gcagaacagt gcgaagaata aaagcgggcg cttcaagctc
     8461 atggttgccg tcgtttccat aagctctttg ccggaccggt ggtgatcttt ttgtacagga
     8521 atacctggaa ggaagcatgc ccgctctgat tgagcgtgat gatttgtcca agggcgacca
     8581 gagccggatg catatgcgtg ccttgcgcat cattcatgat caggctttga gtgggggacg
     8641 ctgagggctt gtcatcctgt ggcgggttga ggctctcgaa aaaggctact ccgatgtata
     8701 gctgcgattt catcaattgg aatgcctggt tgagccatcg gatttgttgg tctgaagggc
     8761 tgtccaaact ctcttcaggc caggagaaac ccgtaaccca tatcaagccg tgagcatgat
     8821 cgtgctgcag cataacctgt cggactgctt cgtagtggcg tagcacctgt cgcgtatctt
     8881 ggttgggcga tgccatcgcc tcacctacga ctgtgctcag acgcaagctc aggatgggca
     8941 tatttgcagc cgcacctgcc tgatagagct cgctcaaata aaccagatca tccatatcac
     9001 ccactgctgg ctgtgccggg agagggctca aaccagcagc taccagcgtt actgggcgag
     9061 cgccagcatt caatgctttt tgactggcca gaagaagctg ggagtatgcg ccggggttgg
     9121 gtgacgcgcc ccatccagct accgtattgg cttgtgggaa gagttcaatc gccatcaggt
     9181 tttgcggata acggttcgcc agcatgacta ccagccccgc ggtttgatta acatcgggtc
     9241 catcagtgcc caacacccag gcaggtgggt gtgaaattga tagcagtacg ttaagatcct
     9301 gtttttgggc tattgccaat gccttgtcca aaggctctag attgatcggg tggttggcat
     9361 ccggccagtg gcgagcccaa tcgaagtcta cgccgatcca atcgataccc accccggcgg
     9421 cagcgtttag cgccagttcc acttcctggc cccacacatc tagacgcgcc ccgtagccaa
     9481 attcagggga attgggcaag cctctggctg cgcgcgctgg ctctgaaacc cccactttgt
     9541 agcttgctag caccccgatg aatagagaaa ccaaagtgat cttgaggaga aggggtttaa
     9601 tcttgtaggt catgctcgga aatcatttgt aggggttttc taatctacac tggcacggat
     9661 ttaattgaat tcactctgct tttcacaaac ctgctaaccc cagtacgatt ccaatcaaga
     9721 atcctgctgc agagagaata agcgcctttt tcagtgttcg ggtatattgt ccagacgtga
     9781 aaccgatgta gcatgtttgg acgaatccct gaatgagctc agagtcgctg agcaatgaaa
     9841 cgacgacaga ccaaaaggca atctcaattc tagaagtaat agtacggatg ctcataagtt
     9901 caagctttct ccaggatgac tcgctcaccg ggatgtggtg acctttagta tctttccttt
     9961 ttcgctaatg accacgcgca tccagcgcgg gatggccttc tcgcctgtgg cggaattcgc
    10021 gcgtgccgtg ccttgaaaag tcagtaaata agttggcgtg atcaatagcg acttgggctg
    10081 cggggcattt tgcaaacgca ccttcgggtt acacccggcg aattccggat agcgtttgtg
    10141 gatttgggca attaccgctc ggagagtatg catgtccatg gcgttatcca ttctctaacc
    10201 cagccaggcg agctcggcgg cgcaggaagg ctggcaagtc caggttgctg ggggagatcc
    10261 ccgatgtcac ttgtgggatg ggctcggtct tttccgttgg aataggcatt ggtttggggt
    10321 ctgaagccag ggcagcctgc tggggtactt tgggagcttc agctttggag ggtgcagcga
    10381 ctttgaatag ggtttcctcc agagtgggag cacccagacc ggtgacgatc agggtaatct
    10441 gcaccctgtc ttccatgcgc tcgtcttgaa ttacgccgag cacgatttca gttcgatttg
    10501 aggtttgctc ttgtagatta gccaatgcgt cttggacctc gaagagcgtc aggtcatcgc
    10561 ccccggtgaa attggcaatg ataccggctg cgttctccag tgcgatggtc tctagcaatg
    10621 gatggtgcag ggcttgttcc agcgccttgc gggtcttatg ttcgccttct ccttgtccga
    10681 tggacatcat ggcgcctcca ccaagttgaa tcaggcgtcg cacatgagcg aaatccacat
    10741 tgatcatgcc cggctcggta atcagctcgg tgattccctg aacggcctgg cggagtacat
    10801 catctgctag gcggaaggcg gtctctatag gcaagttgcg tggagcgatg aacaataacc
    10861 gatcgttcgg gatggcgatc aatgtattcg tgtacttgcg gagtaaatcc aatccttcgc
    10921 tggcgttgcg ctggcggcgg cccatctcga aggaaaaggg cgtggtgaca atggcgattg
    10981 ttaccgcccc aagcgagcga gctatctctg ccactaccgg gatcgcgcca gtacctgtac
    11041 cacctcccat accggcggtc aggaatacca tatccgcccc ttgcaaagcg gaggcgatct
    11101 ctcgacagct ttcttcggtg gcggcgcggc caatactggg gttgccacct gcccctaatc
    11161 cccgcgtaac tcgcgggcca agctggattt tgactggagc cggatttttc tccagtacct
    11221 ggtgatctgt gttggcaacg ataaattcga cgtctttcag gccaagctcc atcatgcggg
    11281 taaccgcgtt accccctccg ccacccaggc ctattacctt taagacgggt tttcggatat
    11341 ctgcaaattg gctttcaaat tcggatggat atgggagggt actcatggga aatgcccctt
    11401 tcggtacaga tttaagtgga tgccagtttt gatgcaattt ccgtgccatc ccctgtgatt
    11461 cgtgtcacct ggcagcctcg ctggcgcatc tcatcaatca gctcttcagg gaatacttgc
    11521 tctcccccca caacggtaat ccgcgccgct aacgcagcct cggcaggtga ataacccact
    11581 gttggattat gtttcaagat aaatgggcgc gccgcatcga gatgccattc agaagcgccc
    11641 caatcatact tgggcagcaa taaatagtga gcaataggat gctcatcttg gggttgtgcg
    11701 ctaataattt tgggctcaac aggtgatgga tgcgtgtctt ccagcgtgct gctgggcggg
    11761 tggagatcag gcttgctctc cagttggtga gctggtgtgg actggtctgg ttgctgagca
    11821 tctccaggtc ttaccagttt gtgcaacgct gccacgatgg gaaggtgata atcttctggt
    11881 tgaaaccaag cctggttagc tgaaggctca gaaggatcgg cagtgagcaa ccaaaaattg
    11941 catgcgagga caaatgggga gatcggttcg cttatgctgt cagatgatgc gcccgccata
    12001 aactgtttgg caatgctcaa gtttatgttg gcatgttcga gttcgtcaat ctctgtcgct
    12061 ggggattcat cggtgttgtg caggcggcga cccgcagcca gcaggaaaat tggcatcggc
    12121 ttgccaagag cggcttgtgc ttcagccaga taccaatcga aaatatgaaa gccgc
//
LOCUS       DBNGSX010000356         3514 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_584759, whole genome shotgun sequence.
ACCESSION   DBNGSX010000356 DBNGSX010000000
VERSION     DBNGSX010000356.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_584759"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..457
                     /gene="smpB"
                     /locus_tag="AC16CB_10685"
     CDS             2..457
                     /gene="smpB"
                     /locus_tag="AC16CB_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR3342978.1"
                     /translation="MGIKIVAKNRKASHEYFLLDTYEAGLVLQGSEIKSIRAGQISLA
                     EAYVQVDGSEAWLMDAHIAPYTQASIYNHEPRRPRKLLLHKVEIRRLWNEVRKKGVTI
                     VPTKVYLKEGRAKVEIALAKGKKLYDKREAIAKRDLQREMERKGRRYEG"
     gene            457..1257
                     /locus_tag="AC16CB_10690"
     CDS             457..1257
                     /locus_tag="AC16CB_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342979.1"
                     /translation="MGPNKQQVYTISSINRLPSEEKKEIYTRLIPSEMLQLFQIDLKS
                     LYREGSNLLKLKAPSGSSVVEMELRHQLDFPDPILYGHMMDTLNGHIHVLLYILNDPN
                     SPRFDVDRLSNGQTTKFGTRARNMDAEQAAMEYGLAPGQVRRGLRLLGSAIQAFERFI
                     ESLGQEMYFTEPLYYHNAVLFEQHGFAYEKGRKLMERIQKGFMQGGDLVPLLDNSTPF
                     RSPLAANSIRLRSWAIHDNLLGETFTDVTMYKRVGKTASINTCLDCTW"
     gene            complement(1265..3508)
                     /locus_tag="AC16CB_10695"
     CDS             complement(1265..3508)
                     /locus_tag="AC16CB_10695"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR3342980.1"
                     /translation="MPNQTLKRLAVLTSGGDAQGMNAAVRAIVRSGLDIGLEVYAIYE
                     GYRGLVEGGQYIRKMTWDSVGGILQRGGTIIGTARSDEFRTRPGRLKAAQNLIENEID
                     NLIVIGGDGSLTGGNIFRQEWPGLLADLVADGQLQQAQADRHPNLLIAGLVGSIDNDF
                     FGTDMTIGADSALHRITEAIDAITSTASSHQRTFVVKVMGRNCGYLALMGALATGADW
                     VLIPESPPAVDNWEDLMCERLKAGRKAGRRDTIVVLAEGARDRYGNYIGSSLVAEILE
                     KCMGEEVRVTVLGHVQRGGAPSAFDRNLGTLLGHAAVDLILSSSPDTEPQVVGLRGNR
                     ITHTPLVECVEKTQAVAKAIENHAYDQAMELRGSSFREIFSTLKTMLRALPHPPQPDQ
                     RRLRLGVIHSGMPAPGMNTAVRAAVRIGLDRGHIMLGMQNGFQGLIDGDIQELDWMSV
                     NGWASLGGSELGTNRKVPSGSDLYHIARNIETHQIQGLLIIGGWAGYESAYHMLQERK
                     NYPAFEIPMVLLPASINNNLPGSDLSVGADTALNNIVEAVDKIKHSAVALRRCFVVEV
                     MGRKCGYLALMSGLATGAERVYIHEEGVTLRDLMTDVDHLNKGFMAGKRLGLMIRNEH
                     ANDVYNTAFMCALFEEEGGGLYDVRQSILGHLQQGGNPSPFDRIQGTRLATRCIDYLI
                     NQANQGSTSCAFIGLQGREIIFHDLQDFPRVGDLENQRPKTQWWMELRHIAKVLAQPA
                     PHSMMDQ"
BASE COUNT          888 a          870 c          856 g          900 t
ORIGIN      
        1 aatgggaata aaaatagttg ccaaaaatcg aaaagccagc catgagtatt ttttattgga
       61 tacgtacgag gctggtttgg tgctgcaagg gagtgaaatt aaatctatac gcgccggtca
      121 aattagcctg gcggaagcct atgtccaggt cgatggaagt gaagcatggt tgatggacgc
      181 tcatattgcg ccctataccc aggctagcat ctacaaccac gaaccaaggc gccctcgaaa
      241 attactttta cacaaggttg aaatccggcg actgtggaat gaggtgcgga aaaagggtgt
      301 gacgattgtt ccaacgaagg tttatttgaa agaagggcgt gccaaagtgg aaatcgctct
      361 ggctaaaggg aaaaaactct atgataagcg ggaggcgatt gccaagcgtg atctgcagcg
      421 cgagatggag cggaagggga ggaggtacga ggggtaatgg gaccaaataa gcaacaagtt
      481 tacactatta gcagtataaa tcgtttgccc tctgaagaaa aaaaggaaat atatacccgg
      541 cttattccat ctgaaatgct gcagttattt cagatcgatt tgaaaagctt atatcgggaa
      601 ggctccaatt tgttaaagct aaaggctcca tctgggagtt ctgtagtgga gatggagttg
      661 cgtcatcagc ttgattttcc tgatcctatc ttatatggac acatgatgga caccctaaat
      721 gggcatatcc atgttttact ttatattttg aacgacccta attcgccgcg cttcgatgtt
      781 gatcgtcttt ctaatggaca gaccacaaaa tttggaactc gggctaggaa tatggatgcc
      841 gagcaagccg ctatggagta tggattagcc cctggtcaag ttcgcagggg attacgtttg
      901 ttaggctcgg ctatccaagc cttcgagcgt tttattgaat cgcttggaca ggagatgtac
      961 tttaccgagc cgctttatta ccataatgcc gtccttttcg agcagcatgg ttttgcatac
     1021 gaaaaagggc gcaagcttat ggagcgcatc caaaagggat ttatgcaggg tggagactta
     1081 gtgccgttgt tagataattc gacacccttt agaagccctc tagctgctaa tagcatccgt
     1141 ttgcgctcat gggcaattca cgataatttg ttgggggaaa cctttacaga cgtgacaatg
     1201 tataagcgtg tggggaaaac cgcaagcatc aatacctgtt tagattgtac ttggtaaagc
     1261 gcaatcattg atccatcatg ctgtggggcg ctggttgggc taagaccttt gcaatatgcc
     1321 gcaattccat ccaccactgt gtttttggac gctggttctc cagatctccc acgcgcggga
     1381 aatcttgcaa atcatggaat atgatttccc tgccttgcaa accgataaaa gcgcaagatg
     1441 ttgacccctg atttgcttgg tttatgagat agtcgataca gcgcgtggct aaacgcgtgc
     1501 cttgaattcg atcaaagggc gaaggattac ccccttgttg cagatgaccc agaatagatt
     1561 gtcgaacatc atacaaacct ccaccttcct cttcgaaaag tgcgcacatg aaggcggtgt
     1621 tatacacatc attggcatgc tcattacgga tcatcaaacc caggcgctta ccagccataa
     1681 agcctttgtt taggtgatct acatctgtca tcaagtcgcg cagggtgaca ccctcttcat
     1741 ggatatatac gcgctctgca ccagtagcta gaccactcat cagggcaaga taaccacatt
     1801 ttcgccccat cacttcaaca acaaagcacc tgcgaagggc gactgcagag tgcttgattt
     1861 tatccacagc ttcgacaata ttgttgagcg cagtgtctgc acctacgctt aaatcagaac
     1921 caggcaggtt gttgttgatg gaggctggca gcaaaaccat gggtatttca aatgccggat
     1981 aatttttgcg ttcttgtaac atgtggtagg cagattcgta tcctgcccaa cccccaatga
     2041 ttaacagccc ttggatctgg tgtgtttcga tgttgcgggc gatatgataa agatcgctgc
     2101 cgctgggtac tttccgattc gtgccaagct cagaaccacc cagggaagcc cagccgttga
     2161 cactcatcca atccaattcc tggatgtcgc catcaattaa gccttggaaa ccgttttgca
     2221 taccgagcat gatatgtccc ctatccagtc caatgcgcac cgcagccctt acagcggtat
     2281 tcatccctgg tgcgggcatt ccagagtgga taactcccag ccgcaagcgt ctttggtcag
     2341 gctgcggagg gtgaggcaat gcacgcagca tggttttgag tgtagaaaat atctccctaa
     2401 agctgctacc gcgcaattcc atggcctgat catatgcgtg attttcaatg gctttggcga
     2461 ctgcctgggt cttttcaacg cattccacaa ggggcgtatg ggtgatgcgg ttgccacgca
     2521 atccgacaac ctgtggctca gtatcaggcg atgaggacaa gattagatcg actgctgcgt
     2581 gacccaggag agttcccaga ttgcggtcga aggcgctcgg tgctccccct cgctgcacat
     2641 gtcccagcac ggtcacgcgg acttcttctc ccatgcattt ctctaatatc tcggctacca
     2701 ggctacttcc gatgtaatta ccatacctat ccctggcgcc ttccgccaat acaacgattg
     2761 tgtcgcggcg cccagctttt ctgcccgcct ttagccgctc acacatgaga tcttcccaat
     2821 tatctaccgc tggtgggctt tcagggatga gtacccagtc agctccagta gccaatgcac
     2881 ccatcaatgc caggtagcca caattccgtc ccataacttt caccacaaaa gtgcgctggt
     2941 ggctggaagc agtgctggtg atggcgtcaa ttgcctcggt gatacgatgc agggcactat
     3001 cagccccgat agtcatatcg gtaccaaaaa aatcgttatc aattgatcca actagaccgg
     3061 ctatcagcag gtttgggtgc cgatcagctt gtgcctgttg cagttgccca tcagctacga
     3121 gatctgcaag caaaccaggc cattcttggc ggaagatgtt gccgccggtt agactgccat
     3181 ccccgccaat gacgatcagg ttgtcaattt cattttcaat gagattttgc gcggctttca
     3241 gtctaccagg acgcgttcga aattcatccg atcgagccgt gccgatgatc gtcccgcctc
     3301 gctgcaggat accacctacc gaatcccatg tcattttacg gatatattga ccgccctcaa
     3361 ccaatcccct gtaaccctca taaatagcat agacttctaa accgatgtct aaccctgaac
     3421 gcacaattgc acgaacggct gcgttcatgc cttgtgcatc tcctccgctg gttagaactg
     3481 ccaagcgttt taatgtttgg ttgggcattg ggta
//
LOCUS       DBNGSX010000357         6522 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1485569, whole genome shotgun sequence.
ACCESSION   DBNGSX010000357 DBNGSX010000000
VERSION     DBNGSX010000357.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6522)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6522)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6522
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1485569"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="AC16CB_10700"
     CDS             complement(<1..995)
                     /locus_tag="AC16CB_10700"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3342981.1"
                     /translation="MGERSARYAHTIGDYQGAAAQAEKAAELAGVAGVKDVEVRAHLT
                     WAYAMLRQGKHEAAVLQAEKGLELARQTGDQHEQSRALNFLGLIAWEQSDTETARRYY
                     EHSLEVARESGNLRIEAAALNNLGGLDGQLGNYSQAIDTYQQSLLITQKIGDQAQQGR
                     VLGNLGWLTGLLGDYTRAGSYCEQNLRVARQAGERENEAVVLANLSSFSERQGDYAAA
                     QTYAQQCLNVARQSGDPSSEAWGQTYAGHAAYGLSQWEAAAQAYQRALEIRRSLDQPD
                     LATEPLAGLARLALASGDIANARAHVGTILSYLDGGGSLTGTDEPLRVYLTCYLV"
     gene            complement(1041..3779)
                     /locus_tag="AC16CB_10705"
     CDS             complement(1041..3779)
                     /locus_tag="AC16CB_10705"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3342982.1"
                     /translation="MHNLVPHFIQDNFKSGKFQGQFPAVGLFLDISGFSTMTVALMAH
                     GPHGAEVLATMMRAALDPLMRSVHAQGGFIATLAGDAFTALFPLASLDEGDGYQRALA
                     AAWAVQETMAAKPEWQTPYGSFVLHAKLGLAVGEVSWGIVTSQDQHRAVSYFRGTAVD
                     GCAQAEHQAQAGEIVMDAAFFSRVAQWVEAQPVGDYYRLTRVNATLPPPQPIRELPVD
                     PKIMAHFYPEGLVSQAHSGEFRQVVNLFIKLPTVRTEAQLSVFMQTLFELQGRYGGLL
                     NRLDFGDKGSNLLFFWGAPVAYENDIERALNFLLALQPLTSIPIHAGVSYQIAHAGFI
                     GSSLLEEYTCYGEGVNLAARLMSAAPRGEIWVDGRIAQRVMDRFELYLEGEMAFKGFA
                     EKQVVYALYDRKEDIEAIYPGELLGREQELDRLEAFVAPLWQGRYTGALVVWGDPGIG
                     KSRLVHAFQTSPLFETHQAVWALCQTDTTLREPFNPFKYWLRHYFAQSDVVTEVRNKR
                     SFNQKLDQLIEETSQIDSGLAGELDRTRSFLGALVDLHWPDSLYEQLDPQGRYENTLT
                     ALTVLLQAESQRGALIIFLEDAHWLDDDSRAFISRLERALTVDESRSYPIAILAAARR
                     EGHIPIFDQRVTVQELDLVGFSSQDLAQLAERILKSSPSPQLLSLLEERSQGNPFFAE
                     QILRYLQEEGHLVLDADGCRPVGTGDQPLLPGDVRAVLISRLDRLGEEVRNVVQTASI
                     LGREFELGLLVRMSQPDERFFQKMAVALEASIWTELSELRYLFLHALLHDTAYQMLVR
                     THRRELHALAVEAIEGLYADGLNLHYGELAYHSEQAELVEKARRYLQLAGDAARDAYQ
                     NNQAVDYYSRAFALTPESDLEGRYHLLLEREALYALLGDSQQRGQDLEALASLAEA"
     gene            complement(3925..4359)
                     /locus_tag="AC16CB_10710"
     CDS             complement(3925..4359)
                     /locus_tag="AC16CB_10710"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3342983.1"
                     /translation="MFTIEKSIFIDRPQQEVFDYVSDAANVHKWQSQMISAEWSSEGP
                     HGIGSTQRAVARFLGRSIEATSEITTWDPPNHHGFKVVSGPFPLEGGMKFVSEGNGTK
                     VTMDGQLEVGGFFKLAEGLVRKQLESQFVTNLEALKLLLEAG"
     gene            complement(4727..4987)
                     /locus_tag="AC16CB_10715"
     CDS             complement(4727..4987)
                     /locus_tag="AC16CB_10715"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HIR3342984.1"
                     /translation="MLVQHQVKNFAEWKKLFDSNIDLRKRSGELSAHVYRNESDPNNI
                     TALFKWDSLANAQKFAHSPELKAAMEKAGVMGQPSIFFLNEA"
     gene            5498..6400
                     /locus_tag="AC16CB_10720"
     CDS             5498..6400
                     /locus_tag="AC16CB_10720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3342985.1"
                     /translation="MVNVLSDRDTPQSKATVLLVSDDLESARIWAYSLEQAGLQVCQA
                     GFSEKTLQVWSSEFPDLIILEDHNARADVFEFCRQIRQEAAVPILVMTSNSQEAHLME
                     AYRAGADDCVPPPVSPRLFMAKVKAWLRRAQVLPNAALAVVQAGEFQLDPDRRMLTTP
                     DGQLLKLTNLETRLLYLFLNHPAWIMETDYLINRVWGHFGDGDGVLLKNLVYRLRRKI
                     EPNPSQPSYLLTESSLGYRFRPEGELSSTVDVPNKGQTGGSGQTQPVIHTTPLQARDS
                     AEGASRGRARDTGQLHLHYPKHND"
BASE COUNT         1392 a         1926 c         1829 g         1375 t
ORIGIN      
        1 aaaaccaggt agcaggtcag atagactcgc aagggctcgt ccgtgccggt caaggagcca
       61 ccgccgtcca ggtaggacag aatcgtccca acatgtgctc tggcattagc gatatcgccg
      121 ctggccaagg ccaagcgcgc cagcccggcc aagggttccg tcgccaggtc gggctgatcg
      181 agcgagcggc ggatctccag cgccctctgg taagcctgcg cggctgcctc ccattgactt
      241 aacccataag cagcgtgccc agcgtaggtc tgcccccagg cttcgctgga agggtcgccc
      301 gactggcggg ccacgttcaa gcattgttgt gcgtaagttt gtgccgctgc gtagtcgcct
      361 tgtctttcag aaaatgagct caggttggcc aggacgacgg cctcattttc ccgctcgcct
      421 gcctggcggg caacgcgcag attctgctcg caataagatc ccgccctggt gtagtcgcct
      481 agcaagccag tgagccagcc caggttgcct aacacgcggc cctgttgggc ttgatcgcca
      541 atcttttgag ttatcaggag ggactgctgg taggtatcga tagcttggct gtaattgccc
      601 aactgcccgt ctaagcctcc caggttgttg agcgcggctg cctcgatacg caaattgccg
      661 ctctctcggg caacttccag gctgtgctca taataacggc gcgccgtctc tgtgtcgctt
      721 tgttcccagg cgattaaacc cagaaagttc aacgcccggc tttgctcatg ttggtcgccg
      781 gtctggcgcg ccagctccaa gcccttctca gcctggagta cagcggcctc gtgttttcct
      841 tgtcgtaaca tggcatatgc ccaggtaagg tgggcgcgca cctccacatc tttgacgcca
      901 gcaaccccgg ccaattctgc tgccttctca gcctgtgcag ccgcaccctg ataatctccg
      961 atggtgtggg cgtatctcgc cgaccgctct cccacctcgg caccccgcgc ccctctccct
     1021 gggcgctcgg tgtgttcgtc tcacgcctcg gcaagactgg ccagcgcttc caagtcttgc
     1081 ccgcgctgct ggctatctcc gagcaaggca tacaacgctt cgcgctccaa caacagatgg
     1141 tagcgccctt caaggtcact ctcgggggtt agggcaaacg cccggctgta atagtcaact
     1201 gcctggttat tttgataggc atcccgggcg gcatcaccgg cgagttgcag gtagcggcgc
     1261 gccttttcaa ccagttcagc ctgttcgctg tgataagcca gctcgccata atgcaaattt
     1321 aacccatcgg catacaggcc ctctatcgcc tctaccgcaa gggcgtgcaa ttcccggcga
     1381 tgcgttctca ccagcatttg gtaagcagtg tcgtgcagca aggcatgcag gaatagatag
     1441 cgcagctcgc tcagctcagt ccatatagag gcttccaagg caaccgccat cttctggaaa
     1501 aatcgctcgt cgggttgcga catgcgcacc agaagcccca attcaaactc tcgacctaag
     1561 atcgaggcgg tttggactac atttctcacc tcttcaccca agcggtccag acgagagatc
     1621 agcacggcgc gcacatcccc cggaagcagc ggctgatctc ccgttccaac tgggcggcat
     1681 ccatctgcat ccagaaccag gtgtccctct tcctggagat agcgaaggat ctgctcggca
     1741 aagaaagggt tgccctgcga acgctcttcg agcaagctaa gcaactgggg tgaaggggag
     1801 cttttcagaa tcctctctgc cagctgcgcc aggtcttggc tggagaatcc caccagatcg
     1861 agctcctgaa ctgtcacacg ctgatcaaaa atcggtatat gcccctcacg ccgggcagcg
     1921 gcgaggatgg cgatagggta tgagcggctc tcatcaacgg tcagggcgcg ctccagacgg
     1981 gagataaacg cccgggaatc atcgtccaac cagtgagcat cttccaggaa gatgatcagc
     2041 gcaccccgct ggctctcggc ctgcagcagg accgtcaggg cagtcaaggt gttctcatag
     2101 cgcccctggg ggtcgagctg ctcgtaaagc gagtctggcc agtgcaggtc gaccagcgcg
     2161 cccaggaaag agcgagtgcg gtcgagctct cccgccaatc ctgaatctat ttggcttgtc
     2221 tcttcaataa gctggtctaa tttttggttg aagctgcgct tgttgcgcac ctcggtaacc
     2281 acatcggatt gagcgaagta atgccgtagc cagtatttga aagggttgaa cggctcccgc
     2341 agggtggtgt cggtctggca aagcgcccat acagcctgat gggtttcaaa taatggagaa
     2401 gtctggaaag catgcaccag gcggctcttg ccgatgcccg ggtcacccca aactaccaac
     2461 gctccggtgt agcgcccctg ccagagcggg gcgacgaatg cctctaagcg gtccagctcc
     2521 tgttcgcggc cgagcagctc gcccgggtag atcgcttcga tatcctcctt gcgatcgtag
     2581 agggcgtata ccacctgttt ttcagcgaaa cccttgaagg ccatctcgcc ctccaggtaa
     2641 agctcaaagc ggtccatcac ccgctgggcg atgcgcccat caacccagat ctccccgcgc
     2701 ggggctgcgc tcatcaagcg cgccgccagg ttgacgccct ccccataaca ggtatattct
     2761 tccagaagcg aggagccgat gaagccagca tgggcgatct ggtagctgac cccggcgtgg
     2821 atgggtatgc tggtcagcgg ttgcagggcg agcaagaaat tcagcgcccg ttcaatatcg
     2881 ttctcataag cgaccggcgc accccagaaa aacaataaat tggagccttt atcgccgaag
     2941 tccagccggt tgagcagacc gccatagcgc ccctgcaact cgaagagcgt ctgcatgaag
     3001 acggacagct gggcttcggt gcgcaccgtg ggcagtttga tgaacaggtt gaccacctgg
     3061 cggaactccc cgctgtgtgc ctggctgacc aggccctcgg ggtaaaagtg tgccatgatc
     3121 tttgggtcga cagggagttc acggatgggt tgtggcggcg gaagggtggc gttcacgcgg
     3181 gtgaggcgat aataatcgcc cacaggctgc gcctccaccc attgagccac ccgagagaaa
     3241 aaggcggcgt ccatgacgat ctcacccgcc tgggcctggt gctcggcctg ggcgcagcca
     3301 tccaccgccg tgccccggaa atagctgacc gcccggtgtt ggtcctggga ggtcacaatg
     3361 ccccagctca cctctcccac cgccaggccc agcttggcat gcaggacgaa actgccataa
     3421 ggggtttgcc attctggttt ggctgccatg gtctcctgga cagcccaggc agctgccagg
     3481 gctcgctggt acccatcccc ttcatccagc gaagcgagcg ggaaaagggc ggtgaacgca
     3541 tccccggcca gtgtggcgat gaagccgccc tgggcatgca cgctcctcat cagcgggtcg
     3601 agggcggcgc gcatcatagt agccaagacc tcggcgccat gcgggccgtg cgccatcagg
     3661 gctacggtca tggtcgagaa gccggagata tctaaaaaca gtcccacggc agggaactgg
     3721 ccctggaatt tgccggattt gaaattatcc tgaataaagt gggggacaag gttgtgcatg
     3781 gcggagtaat tatactgcgg ttatgcccac cccctcgttc cccctccctt ccgccttggt
     3841 agcgggaggg ggatgaaaga cggaaaaacg gggcaatcct ttgtctttac aaaccgatct
     3901 caaatgcgtc cgggacgttt actcctaacc tgcctccagc agaagcttca aggcttccaa
     3961 attggttaca aactgacttt cgagttgctt tctgaccaaa ccttctgcca gcttgaaaaa
     4021 tccgcctacc tcaagctgtc catccatggt cactttcgtg ccattcccct ctgatacaaa
     4081 tttcattcca ccttctagtg gaaatggacc actaaccacc ttgaaaccgt gatgattggg
     4141 cggatcccag gtagtgattt ccgaggtggc ttcgatactg cggcccagaa agcgggcaac
     4201 agcgcgttgg gttgagccga tgccgtgcgg accttcggag ctccattccg cagatatcat
     4261 ttggctctgc catttatgca cgttggcagc gtcggacaca taatcaaaga cttcttgttg
     4321 cgggcggtcg atgaatattg acttttcaat tgtaaacatt gtatgttctc ctttggctat
     4381 tagaaatgga cattaatctt agttgttcgt gttttagtag tgcaacacat tttagtagtg
     4441 caacacaaat gaatattgac tgatagtttt ccttcctttc tccgatagat aaaattggta
     4501 aacccgtgtt tgctagacga gaggcctata tgaggaggcg acagtgcgct gtcaaaaaca
     4561 gcaatgcagg cgatgacgaa gcaacccctc ttaatcaggt cgggggattg cttcgaacct
     4621 ggaaagagca ccaggttctc gcgatgacat gcgaattttg tttggttggt taaataaagc
     4681 tgcccctccg gagatccaga agggcagctt cttggtcatc taatttctag gcttcgttga
     4741 ggaaaaagat ggagggttgg cccatcacgc cggctttctc catcgccgct ttcagctcgg
     4801 gtgaatgggc aaatttttgg gcgttcgcca gcgaatccca cttaaaaagt gcggttatat
     4861 tgttcggatc gctttcgtta cgataaacgt gtgccgagag ctcgccgctt cttttgcgca
     4921 aatcgatatt tgaatcaaat aactttttcc actctgcgaa gttctttact tgatgttgaa
     4981 ctaacatact ggacatgctg attttctcct tgtttaagat ttgggcaaac tggaacttct
     5041 gccggatggc gcccagagca cattgcgtag ggaatggccc ccaaaaaaaa acgggcaacc
     5101 tgaccttgaa tcaagttacc cgggagggtg gcctaaatca gcagttgtat atcaatttga
     5161 ccttactaat ccatattata ccacgggaac tcattcgaga gatgatgatt ggatgagtgt
     5221 tcaagggcaa tcgcattaga gcgatttccc ccctggatat ggggaaaaag agtttccgaa
     5281 cgatctcttt gtatacgccc accccctcga tccatctccc tgccgccctt caggctacgc
     5341 ccgcaggggg ttaggggtgg gtgggggttg tggtgaggac gcggcgaatc tccaccctca
     5401 aatactgtga ctttagtgta accagacagg ttaagagtcg tttaatagga tgcacaaagc
     5461 aacccaacct tgtgcatcct attcgtgaga ttacccgatg gtcaatgtcc tcagcgatcg
     5521 tgacacgcca caaagcaagg cgacggtgct cctggtgagc gacgacctgg agagcgcccg
     5581 catctgggcc tacagcctgg agcaggctgg gctacaggtc tgccaggccg ggttcagcga
     5641 gaaaaccttg caggtatggt ccagcgagtt ccccgatttg atcatcctgg aagaccacaa
     5701 cgcccgtgcg gacgtctttg agttttgccg ccaaattcgg caggaggcgg cggtgccgat
     5761 cttggtaatg acctccaaca gccaggaggc gcacctgatg gaggcctacc gggccggggc
     5821 ggatgactgc gtgccgccgc cggtcagccc gcggctcttc atggccaagg tgaaagcctg
     5881 gctgcgccgg gcgcaggtgc tgcccaacgc cgccctggct gtcgttcagg cgggcgaatt
     5941 ccagctcgac cccgaccgcc gcatgctgac cacccccgac ggccagctgc tgaagctcac
     6001 caacctggag acacgcctgc tgtacctgtt cttgaaccac ccggcctgga tcatggagac
     6061 ggattacctg atcaaccgcg tgtggggtca cttcggcgac ggggacggcg tcttgctcaa
     6121 gaacctggtc taccgcctgc ggcgcaagat cgagcccaac cccagccagc cgagctacct
     6181 gctcacagag agcagcctgg gctaccgctt ccgcccggaa ggggagctct cctcaacggt
     6241 ggatgtgcca aacaaaggcc aaacaggggg gtcaggccaa acgcagcccg tgatccacac
     6301 caccccgctc caggcacgcg atagcgccga gggcgcttcc aggggacggg cgcgcgacac
     6361 cggccagctt cacctccact accccaagca caacgattaa cccagcgccg cgcggtgcaa
     6421 aaaatttgcg accggctgtg accggttctg cgacgcattt aacgaccccc gtaggctaag
     6481 atgaagtgga atgaatggaa ctgaagaccc cgccgacgag cg
//
LOCUS       DBNGSX010000358         8009 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_654975, whole genome shotgun sequence.
ACCESSION   DBNGSX010000358 DBNGSX010000000
VERSION     DBNGSX010000358.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8009)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8009)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8009
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_654975"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="AC16CB_10725"
     CDS             <1..326
                     /locus_tag="AC16CB_10725"
                     /inference="COORDINATES: protein motif:HMM:NF014674.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR3342986.1"
                     /translation="RGEINHNTGKLVLADAFQTGRAPQEIVAERGLGQISDASVIAEL
                     VKKVLAENPEQVAAYLGGKSGVSRWLFGQVVRAAGGQANPQVVQLELDRQLEDLKNKD
                     LPSTS"
     gene            541..1827
                     /locus_tag="AC16CB_10730"
     CDS             541..1827
                     /locus_tag="AC16CB_10730"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR3342987.1"
                     /translation="MSETINAFTMAQSQFDHVAKLLNLDPQVAEMLRWPNREFKFMIP
                     VRMDNGTIRVFFGYRVQHSDVRGPAKGGIRFHPAETLDTVRALAMWMTWKTAVADIPL
                     GGGKGGVPVDPATLTISEKERLCRGWVDQMWRNIGPRQDVPAPDVGTTPQMMGWMMDE
                     YSKLVGQYTPGVITGKPLGGGGSLGRTEATGYGVIYTVREAMKHLKMDPTKSVAAIQG
                     FGNVSQYAAIGFVEQLGGKVACVSYWDREDRVAHTVSHAKGIDPSFLQSITDIYGAID
                     KDKARQAGYTIEDGGDWISKDVDVLIPGALEGQINADTVKRISSRVKLVAEGANGPTT
                     PDADTVLKGNGIFVIPVFRCNSGGVTTSYFESVQNDMNYYWTRDEVLERLDTKITQAF
                     NSVVEMAEKEKVYMRDAAYMVAIDRVVKAMELRGWI"
     gene            complement(1906..2286)
                     /locus_tag="AC16CB_10735"
     CDS             complement(1906..2286)
                     /locus_tag="AC16CB_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342988.1"
                     /translation="MATDQTQLEVTEKKVLNKRLESIGWGCFLIMLGGFALVPEAVVP
                     KGLWSIGVGVIMLGLNIARYFSQIKMSGFTTFLGVIALISGVMQLLGVHSFEGAILLI
                     ILGAYLLVKPWFDRRQLFGKAEDS"
     gene            complement(2342..3868)
                     /locus_tag="AC16CB_10740"
     CDS             complement(2342..3868)
                     /locus_tag="AC16CB_10740"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR3342989.1"
                     /translation="MAQISQAQMRGRAYPRMNIVRLGLGIALSALSGVMLLLAFPPYG
                     LWPLAWFALVPGLLAQYRLLPAKWTSLASAVYSGVWLGPFLARLFGPGYGPIFQYLGV
                     WIAILNLFLAKDRKFHELSKYRWFILQGVIVWVGFEMIRATFIPVIATSAFIGYTQAT
                     QTWLIQPVSIFSVYALNLVIILINYALAQGAMAWLDRKWTAADVVPVDADSTRRWLVG
                     TSVVLAVWIGLSLVIVNAMPSTPKLRVASVRPNFPLPAHLDDVNTSQIRFDTFVAQAR
                     EAAAQGAQVIYTPEMMFNFDPQVEYTEQFRALAKETGAYIFITYSVSIEGQDWRNESV
                     MLSPEGEFSQVYGKYHTWMIGEPPTPSAGVFPVYETPLGKLAGMICHDANYTDVARKL
                     TANGAQIIAAPYREFGGFGEQAWTNALFRAVENRVAVVVSGVATVSAIVNPDGSIVDL
                     DTDINGSRAILVGDVRLGPGNAPYTSLGDVLGWASLAGYVFFMFFPSVIERRAKKAQG
                     "
     gene            4319..>8009
                     /locus_tag="AC16CB_10745"
     CDS             4319..>8009
                     /locus_tag="AC16CB_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342990.1"
                     /translation="MKCPNCNHISETALMQCSLCGEAYDRHSLETFQHLEYLLSWLDS
                     RAETLDAATLKNLRADAMAESQRLLDELLPAPEPAAVIEPSVEEISLSPDAERELALI
                     LATRTLIPGWIEAKLIGGRTAGELGDHLTRQAQAIHAQAGIIELELPEPSEADVHTFA
                     MGALDGWIEQKLFTASDASLLRDCLRENLGLTLQPAPVPAAISPPAAPAVRRPVVKAA
                     PPRPPRPPFDWGKLWGSVVEAAVSGLLLRWLRFLGAFLFVVSLAIVVISFWTSIPAWG
                     QLGIIFFIPTAFYAGGWYLRAKMQIEQTGSVLMGVGEMVLAVAFAAIYQFSSFTIPLP
                     TYWLVTSAICTIVYVLTTWRLVHSEFFSYISLVGISSTLMALGQVLRLPIEWQLVILS
                     ASSLGMVETSARFKGGDPRWGDVSLGARRFPQLLLLLVLGLLLLIPWEGAYPAQAVGF
                     ALGAAACGLLAWRFPHRVYAHLAGWGSIVAIGLSLRAASIPWHWYPSAAAVLSPLYIL
                     TGIVLSRRIPESFQLKTSYLLVFKLAGFSLLAVGIAAGVATLNFAFWSGVVALAVASI
                     IMAGYAPLLRQPLYVLGSGGLLLLPYSLSVSQWLSDSQVAQLGTWVMAACAGLALAYL
                     GIGSGLRRLPRYAEFLHLLSHMLAPLVAIGLLVVETMARSDSTIPSLVTLSGILLVYG
                     ISAAQHDSQNFPGLSRWVSWLPDGFRQSVFLYPIGVLIPIWVAVAWSGSVLTPEWLGV
                     ALAGIAVCYAGVGQFITRRQVAYRLPFHLLAYPIGVVAVWAAFQNQVALITTLYLNVA
                     ALVAMAAIYRRFHETTLAGLLLLWPFQLSLNLSQITPHAQSLAYTLLVGLGYTPLGMW
                     LQKRGDKYFYLPVYLIGYGISIYALVASLLGRFGAYPLDLPWIGAVVPLLVTGLYSFS
                     AYWLRKPVFAWGSAATFTLAFGQALTLMRVPAQYDASAWVALGMAYILLASRLRGSAP
                     ERSGDGWLAAFRLPLNVGAWAVAASGVLLTYPATLDALFQTRAENLPATMLAQWLAVV
                     FVVLSTALYRNPWPLLLEPPLAFIPVTLLFIGYGERMLGQALSPAQLGVVWAGLGLVH
                     FGISVGLDQVRSKTRVSPGGVYLGGYAMAAFSVLWTILDRGSLLWALGLWVFTSVSSA
                     WLVHLGRHTVWDNLVRTLFGSKESALKSSLRAAFLWLGAWPFPVWLFLLLAHLGVVES
                     FQWLGFGLCGLAFVGLAAWLRPLERT"
BASE COUNT         1650 a         2322 c         2266 g         1771 t
ORIGIN      
        1 tgcgcggcga gatcaaccac aacaccggca agcttgtgct ggcagacgct ttccagacag
       61 gcagagcacc ccaggagatc gtcgccgagc gaggcttagg ccagatctcc gatgccagtg
      121 tcattgcaga gctagtgaag aaggtattag ctgaaaaccc agagcaggtg gctgcctacc
      181 tgggcggaaa gagcggagta tctcgctggc ttttcgggca ggtggtacgc gctgccggcg
      241 ggcaagctaa cccgcaagtg gttcagctgg aactagaccg acaactagag gatttgaaga
      301 acaaagacct accctctaca agctagaaag cttcggcaag taataacatc gttaaacgtg
      361 aagttcatca cttgacgatc tgtcggacat ccattaaggt taacgtacag gttctgtaat
      421 accaaggctt agtgatgagc cagtcacgac tcataatcca ttacgctggc aaacctttgg
      481 cgggcaccat accggggtgt ccgctttata tttaacgtct catgtcatag gagaaagaca
      541 atgtcggaaa cgattaacgc cttcacgatg gctcaaagcc agttcgatca cgtcgccaag
      601 ctgctcaacc tcgacccgca agtggcagag atgctgcgct ggcccaaccg tgagtttaag
      661 ttcatgatcc cggttcgcat ggataacggc accatccggg ttttctttgg ttaccgcgtg
      721 caacacagcg atgtgcgcgg acctgcgaaa ggtggcatcc gctttcatcc tgcggagacg
      781 ctcgacaccg tgcgcgccct ggcgatgtgg atgacctgga aaaccgcagt ggcggatatt
      841 cccttagggg gtggtaaagg cggcgtgcct gtagaccctg ccacgctgac catatctgag
      901 aaagagcgat tgtgccgcgg ctgggtagac cagatgtggc gcaatattgg cccgcgccag
      961 gatgtcccag cccccgatgt gggcaccaca cctcagatga tgggctggat gatggatgag
     1021 tactccaagc tggtaggtca atacacgccc ggcgtgatta cgggtaaacc gctgggcggc
     1081 ggcggctccc tcgggcgcac cgaagccact gggtatggcg taatctatac cgtgcgtgag
     1141 gccatgaagc atctgaagat ggatcccacc aagtcggtgg cggctattca aggctttggc
     1201 aacgtctctc aatatgccgc aataggcttt gtggagcaat tggggggtaa agtcgcctgt
     1261 gtctcttatt gggatcgaga agaccgtgta gcgcacaccg tcagccatgc caaagggatt
     1321 gacccgagtt tcttacagag catcaccgac atctacggcg ctatcgacaa agacaaagcc
     1381 cgccaggctg gttataccat tgaagatggc ggcgactgga tcagcaagga tgtggatgtg
     1441 ctcatccccg gggcgctgga agggcagatc aatgcagaca cggtcaagcg catcagcagc
     1501 cgcgtgaaac tggtagcgga aggcgccaat ggtcccacca ccccagatgc ggataccgtc
     1561 ctcaagggca atggcatttt tgtcatcccc gtcttccggt gcaactctgg tggcgtgacc
     1621 acctcgtatt tcgagagcgt tcagaacgac atgaactatt actggacacg cgatgaagtt
     1681 cttgagcgcc tggacaccaa gatcacccag gccttcaata gcgtggttga aatggctgag
     1741 aaagaaaaag tctatatgcg cgacgccgcc tacatggtcg ccatcgaccg cgtcgtcaaa
     1801 gccatggaac tgcgcggctg gatatagatt atcggagggc aggggattgg ataaacgagc
     1861 aattgggcgg cgacatcgcc gcccaattgc tcgtttatca acagtttagc tatcctcagc
     1921 cttgccaaag agctgccgcc tatcgaacca cggtttaacg agcaggtacg cgccgaggat
     1981 gatgagcagg atcgccccct cgaaagagtg cactcccaac agctgcatca ccccgctaat
     2041 taatgcaatg acacccaaga aggtggtgaa gccgctcatc ttgatctggc tgaagtagcg
     2101 cgctatgttc agccccagca tgatgacgcc cacccctatc gaccaaagac cctttgggac
     2161 caccgcctca ggtactaagg cgaacccgcc caacatgatc aggaaacacc cccatccgat
     2221 cgactccagg cgcttgttga gcaccttctt ctctgtcact tcgagttgtg tttgatctgt
     2281 agccattttt agatctccta cttgaattcg gccgcgtctc aacacaggga gcccttatct
     2341 attaaccctg tgccttcttg gctcgtcttt ctatcaccga tgggaagaac atgaagaaga
     2401 cataacccgc tagcgaagcc cagcccagca catcgcctag cgatgtgtaa ggagcattgc
     2461 cggggccaag gcgcacatcg ccgaccaaaa tcgcccgcga gccgttgata tcggtatcca
     2521 gatccacaat actgccatcc gggttgacga tggccgagac cgtcgccacg cccgatacga
     2581 ccaccgccac gcggttttcg acggcacgga acagggcgtt ggtccaagct tgctcgccga
     2641 accctccaaa ctcgcgatac ggggcagcga tgatctgcgc gccgttagcg gtcagcttgc
     2701 gggcgacatc ggtataattg gcatcgtgac agatcatacc cgccaatttc ccaagcggcg
     2761 tctcgtagac gggaaacacg ccggcagatg gcgtgggcgg ctcgccaatc atccaggtgt
     2821 gatacttgcc gtacacctgc gagaactccc cttcaggcga gagcatgact gactcgttgc
     2881 gccagtcctg cccctcgatc gagacagagt aagtgatgaa gatgtacgca cccgtctcct
     2941 ttgcaagcgc gcgaaactgc tccgtgtact ccacctgtgg gtcgaagttg aacatcatct
     3001 caggggtata gatgacctgc gccccctgcg ctgccgcttc acgcgcctgc gctacgaacg
     3061 tatcaaagcg gatttgcgac gtattgacgt catccaagtg agctggtagc gggaaattcg
     3121 ggcgcacgct tgccacgcgc aacttaggcg tacttggcat cgcattgacg ataaccaggc
     3181 tgaggccaat ccacaccgcg agaacaacgc tcgtccctac cagccagcgc cgcgtcgaat
     3241 ctgcatccac ggggacaaca tccgcagccg tccatttgcg gtcgagccag gccatggcac
     3301 cttgagccag tgcatagttg ataagaataa tgaccagatt cagggcatag acgctaaaaa
     3361 tcgagacggg ttggatcaac caggtctgtg tagcttgggt atagccgata aaagcgctcg
     3421 tcgcgatcac agggatgaac gtggcgcgga tcatctcaaa acccacccag acgatcacac
     3481 cttgcaagat gaaccaacga tatttgctta actcgtggaa cttgcgatcc ttggccagaa
     3541 aaaggttaag gatggcgatc cagactccca agtattggaa gattggtcca taaccaggac
     3601 caaagagccg cgccaggaat ggacccagcc atacgccgga gtagaccgct gacgccaggc
     3661 tggtccattt ggctggcaag agcctgtatt gagccaacaa gccaggcaca agcgcaaacc
     3721 aagcaagcgg ccacagtcca tagggcggga aagccagcag cagcatcacg ccgctcaggg
     3781 cagacagggc aatccccaac cccaagcgca ctatattcat cctgggatat gccctccccc
     3841 gcatttgtgc ctggctgatc tgagccataa caacctcccc tccacctggt attactttac
     3901 aatatttggt agccagagtc aacctgagcg cgcccaggct cgggcaaaag catggatgaa
     3961 tttctaacca aaagtcatcg acatcctttt gtccgcaccc ccgttttatt tgtgaaaaaa
     4021 attattaact tgatatttgg tgaaaattta tataaaatct aattgaaatt ctccacaatc
     4081 ggatggcatg ttgaagtaga ttttcacttt ccctcactac acttaacgcg gcactccaat
     4141 gattgcaagc agtcagaatc gacttgatca tcgccccgca tgacccttcg cttaaaaggg
     4201 acaccagcgg ggctcgtttt ttttctgggg tggctttttt agacggttgt gaactttcac
     4261 agagcttgta agctgatcga ccacgaagca cgacactccg ttcattggag gccaagtcat
     4321 gaaatgtccc aactgcaatc acatctctga aactgcgctg atgcagtgca gcctgtgcgg
     4381 tgaagcctat gaccgccatt ctctggagac cttccagcac ctggaatact tgctctcctg
     4441 gctggactcg cgcgccgaga cactagatgc ggccaccctg aaaaacctgc gggctgacgc
     4501 catggctgag agccagcgct tgctggatga gcttctccca gccccggaac ccgcagccgt
     4561 catcgaacct agtgttgaag agatctcgct cagccctgac gcagagcgcg aattggcgct
     4621 gatcctggct acccgcacct tgatccctgg ttggatcgaa gccaaactga tcggaggtag
     4681 gacagctggc gaactaggcg atcacctgac caggcaagcc caggcgatcc acgcccaggc
     4741 agggatcatc gagttggaac tacccgagcc ctcggaagct gacgtacata ctttcgccat
     4801 gggtgcgctg gatggctgga tagagcaaaa gctatttacc gctagcgatg caagcctgtt
     4861 gagggactgt ctgagagaaa acctggggtt gaccttacaa cctgccccgg taccagccgc
     4921 gatctctcca ccagccgcgc cagccgtacg ccggcccgtg gtcaaggctg cacccccccg
     4981 ccctccacgc ccgcctttcg attgggggaa gctgtggggt agcgtggtgg aggcagcggt
     5041 ctccgggttg ctgctgcgct ggctgcgctt tctgggcgct tttttattcg tcgtctccct
     5101 ggccatcgtc gtgatcagct tttggacgag catccccgca tgggggcagc tggggatcat
     5161 tttcttcatc ccgactgcgt tctacgctgg cggctggtac ctgcgcgcca aaatgcagat
     5221 cgaacaaacc ggcagcgttt tgatgggggt gggcgagatg gtcctggcag tggccttcgc
     5281 cgcaatctac caattctcca gctttaccat ccctctgcca acctactggc tggttacttc
     5341 cgccatctgt actatcgttt atgtgctgac cacctggcgg ctggttcaca gcgagttttt
     5401 cagctacatc agcctggtcg ggatcagcag cactttgatg gcgctgggtc aggtgctccg
     5461 tctgcccatc gaatggcagc tcgtcatctt atcagccagc agcctgggta tggtggaaac
     5521 ctccgccagg ttcaaaggcg gcgacccccg ctggggagat gtgtcgctcg gcgcgcgcag
     5581 gtttcctcag ttgctactct tgcttgtgct gggcctgttg ttgctcatcc cctgggaggg
     5641 ggcttatcct gctcaagcag taggcttcgc cctgggcgcg gctgcctgcg ggctgctggc
     5701 ttggcgcttc ccgcaccgcg tctacgcgca cctagctggt tggggttcca ttgtggccat
     5761 tgggctgagc ttgcgggctg caagcattcc ctggcactgg taccccagcg ctgccgcagt
     5821 tctatccccc ctctacatac tgaccgggat agtcttgagc cggcgcatac ccgaaagctt
     5881 tcagttaaaa acatcctacc tgctggtgtt caaactggct ggcttcagcc tgctcgccgt
     5941 ggggatcgca gcaggtgttg cgacgctgaa ctttgccttc tggtctggtg tggtggcgct
     6001 ggctgtggct tcgataatta tggccgggta cgcccccctg ttgcgccagc cgctgtacgt
     6061 attgggatcg ggcgggctgc tcctgcttcc ctattctttg agcgtttccc aatggctgtc
     6121 agacagccaa gtcgctcaac tgggaacctg ggtgatggct gcctgtgctg gtctggccct
     6181 ggcttatctg gggatcggct cagggttacg cagactgcct cgctacgccg aatttttgca
     6241 cctgctatct catatgctgg cgcctctggt agccatcggg ctgctggtcg tcgaaacaat
     6301 ggcgaggtcc gactccacca tcccctctct cgtaaccctg agcgggatct tgctggtcta
     6361 tggtatttcc gctgctcaac acgacagcca gaatttcccc gggctctcaa gatgggttag
     6421 ctggctgcca gacggatttc gccaatccgt attcctgtac ccgattggcg ttttgatccc
     6481 catctgggtg gcagtagcct ggtctggaag cgtactcaca cccgaatggc ttggggtggc
     6541 gctggccggg atcgccgtgt gttatgcggg tgtgggtcaa tttatcactc gccgccaggt
     6601 cgcctatcgc ttgcccttcc atctgttagc ctacccaatc ggtgtggttg ccgtctgggc
     6661 agctttccaa aatcaggttg cgctgatcac tacgctctac ctgaatgtcg ctgcgctggt
     6721 ggcaatggcc gccatctacc ggcgctttca cgagacgacg ctggctgggc tgctgttact
     6781 atggcccttc cagctctcgc tcaacctgag ccagatcact ccccacgccc aatcactggc
     6841 ttacacttta ctggttgggc tgggatacac gccgctgggg atgtggctgc aaaagcgagg
     6901 ggacaaatat ttctatctgc ctgtgtacct gattggatat ggcatcagca tctacgccct
     6961 ggtggcatcc ctgttggggc ggttcggggc ttacccgctg gatctaccgt ggatcggtgc
     7021 tgtggtgccg ctgttggtga cagggctgta tagctttagc gcatattggc tgcgcaagcc
     7081 ggtatttgcc tgggggtccg cagcgacatt caccctcgcc ttcggacagg cattgacttt
     7141 gatgcgtgta cctgcgcaat acgatgcaag cgcctgggtg gccctgggca tggcctacat
     7201 cctgctggca agccgacttc ggggatctgc cccggagcgc agtggagatg gctggctggc
     7261 agccttccgc ctgcccttga acgttggcgc ctgggcagtg gctgcctcgg gggtgctgct
     7321 cacctaccct gccactctcg atgcgctctt ccaaacgcgc gccgaaaacc tgcctgcgac
     7381 aatgctggcg caatggctgg cagtcgtctt cgttgtgctg agtacagcac tgtaccgcaa
     7441 cccctggccg ctgctcctgg agccaccatt ggcattcatc cctgtgacct tgcttttcat
     7501 cggttatggc gagcggatgt tgggccaggc attgagtccg gcccaattag gtgtcgtgtg
     7561 ggcgggtctg gggctggttc atttcgggat atcagttggc ctcgaccagg tccgctcaaa
     7621 gacacgcgtc tcgccggggg gcgtgtacct gggtgggtac gcgatggctg ccttctcagt
     7681 gctatggacg atattggacc gtggttcgct gctgtgggca ctagggcttt gggtattcac
     7741 gtcggtttcc tcagcctggt tggtgcacct cggacggcac acggtctggg ataacctggt
     7801 caggacattg ttcggctcca aggaatccgc cctcaaatcc agcctgcgag ccgccttcct
     7861 ctggctgggg gcctggccat ttccggtatg gctgtttttg ctgctggccc acctgggagt
     7921 ggtggaaagc ttccaatggc ttggttttgg cctgtgcggg ctggcatttg tgggcctggc
     7981 agcctggctg cgacccctgg aacgcacgt
//
LOCUS       DBNGSX010000359        17051 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_377505, whole genome shotgun sequence.
ACCESSION   DBNGSX010000359 DBNGSX010000000
VERSION     DBNGSX010000359.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 17051)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17051)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17051
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_377505"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..516
                     /locus_tag="AC16CB_10750"
     CDS             7..516
                     /locus_tag="AC16CB_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HIR3342991.1"
                     /translation="MTLRIGILTVSDRSSRGERADTSGPAIQAVVERQGWVVSKTGII
                     PDHLEAIQEMLIDWVDSEEVDVILTTGGTGFSRRDVTPEATLAIIERAAPGISEAMRS
                     ASLQVTPHAMLSRAMAGIRGRTLVINLPGSPKGAVENLQVVLPVLSHAVQLLRDEPDA
                     EAGHMHHDG"
     gene            complement(566..1975)
                     /locus_tag="AC16CB_10755"
     CDS             complement(566..1975)
                     /locus_tag="AC16CB_10755"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR3342992.1"
                     /translation="MKLRINLFLLMCLLFAVLPVRAQEEQPQGPVYTVQAGDTLGGIA
                     RRFGLTIDELALANGISNPNLLSEGTQLVIPGLDGIQGFLTTVTVPFGENLSSISRRY
                     QIDIALLARLNHLTAPSELYAGADLVIPQEETGATNLTRMALAEGQSLLELSILAGTN
                     PWSVLRGNQIMNSWGALPGDVYLLPGDGEAGPGGLPGAIQDVEITPLPLRQGKAGVIK
                     IAASQGLELEGSFMGYPLNFFSDTEGGYVALFGIHAMTEPAFYPFHLQGKLSDDRPFT
                     FTQRVGVRLEDYPFDQPLTVDPATIDPAITRPEDAQWSALAATATPERLWDGLFRMPS
                     PLSQDYCVETNECWTSRFGNRRSYNGSPYAYFHTGLDVAGGTGTKIFAPAPGVVVFAG
                     PLTVRGNATMIDHGWGVYTAYLHQSEIMVEVGDRVESGDEIGLVGGTGRVEGPHLHWE
                     VWVNGIQVDPLDWLIQPFP"
     gene            complement(1959..2336)
                     /locus_tag="AC16CB_10760"
     CDS             complement(1959..2336)
                     /locus_tag="AC16CB_10760"
                     /inference="COORDINATES: protein motif:HMM:NF014678.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HIR3342993.1"
                     /translation="MKNIVIQNTSRPTSKPVRAKYCSSFMCRLRGFTFRRNLTSDEAL
                     LLVQLKDSRLDAAIHMLAVWTDLAVIWINNQGVVVDLCTARAWRPFYIPKQPARYILE
                     MAPSRLSEFAIGDKVTLEDETED"
     gene            complement(2420..2797)
                     /locus_tag="AC16CB_10765"
     CDS             complement(2420..2797)
                     /locus_tag="AC16CB_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3342994.1"
                     /translation="MLDNPKQVVYIEDEQEMIELVRLILSRKGYEVLGASGGREGLDL
                     VRQILPDLVLLDLMMPDMDGWEVYQQMRAEEATRDIPVIVITAKAQSIDKVLGLHIAK
                     VDAYISKPFSPHDLVESVERVLS"
     gene            2988..3791
                     /locus_tag="AC16CB_10770"
     CDS             2988..3791
                     /locus_tag="AC16CB_10770"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3342995.1"
                     /translation="MTRSSPVLLVTGASSGIGEATAKLFAAHGYRVVLAARRRERLEA
                     LGEEIRKMGGDALPVVADLTDLRQIEQLVDSSLSDYGRIDVLFNNAGMGRMNWLENLD
                     PVADIEALFRVNLLGVVQTTRAVLPHMIMRKSGHIINMASMAGYLATPTYSIYAASKF
                     AVRGFSDALRREVGVYGIRVSGIYPSGVETEFARHAGIKRKTGISTPARLRLSAEQVA
                     ISVLDVVHRPRRAVLIPAPMRMVAWFGFFFPGFVDWIIEKRFTRPERGL"
     gene            complement(3843..4301)
                     /locus_tag="AC16CB_10775"
     CDS             complement(3843..4301)
                     /locus_tag="AC16CB_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3342996.1"
                     /translation="MDILHLIDRLEELLNQSRPFPFTHNVIIDEDRMLDIIDQMRVAI
                     PEEIKKAQQVMNQRDRILAQAQEEANRTITIAREKLDQLVERDAIVQAAHAKAGEVLA
                     MAQVERESTRKEADEYALDTLRRLEMELERFLNQVRNGISVLHSEKQEPE"
     gene            complement(4390..4914)
                     /gene="coaD"
                     /locus_tag="AC16CB_10780"
     CDS             complement(4390..4914)
                     /gene="coaD"
                     /locus_tag="AC16CB_10780"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR3342997.1"
                     /translation="MVRAVFPGTFDPIHLGHIDIADRATQLFDELVVAVYDRPLKSLL
                     FSPEERIALVKQSFKDNPKITVMGYSGLTVEYCQAIGAHVIVRGLRVFSDFEYEFRMA
                     LANHRLAPNIELVALITAEEHTFLSSSTVREIASLGGDVSSMVPEHVKLALQARFTEL
                     GDGQQLVPTTSLRD"
     gene            complement(4914..7439)
                     /gene="recG"
                     /locus_tag="AC16CB_10785"
     CDS             complement(4914..7439)
                     /gene="recG"
                     /locus_tag="AC16CB_10785"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR3342998.1"
                     /translation="MKPSLDKLHKFFKLEAERGYDNRAVMGGLERVLDNWEAEARSDN
                     LSEELIQSIRSQLRDYSRLDENARAEVLTRIWDRVQENSSSSLPTLPTPSKEEKTDST
                     PAKPAEPTPSKIDLPPHLHGPQHQPQVQIDKSETASTVETEVPAALNAPTTVLPNIGP
                     RHAQTLSRLGMHTLRDMMYYFPRKYLDYTQLKPINRLWYGEEVTVIGTVQNITSRPRR
                     GRPSQIAEAVISDGTGALRVTWFNPWLAKRLQTGAHISLSGKIDQYLGRLVMVNPEWE
                     TLEQQQLSTNRIVPMYSLTAQITQRWLRRLMDQVITYWSPRIPDHIPEKIRQNADLLD
                     LPTALQQIHFPENWDLLKAARHRLAFDEIFLLQLGVLRQKRMWQERTARAFEVEDNWL
                     ATQIAGLPFPLTEAQQRAVNDLRTDLASGIPMNRLLQGDVGSGKTVVAALAISIVTHL
                     GAQAALMSPTSILAGQHYKSMMSLLAGVNGQATQPEGGYPIRPEAIRLMIGATPEAEK
                     REIREGLAEGSIKLVIGTHALLEDPVTFSNLQLAIVDEQHRFGVEQRATLRSKGENPH
                     LLVMTATPIPRSLALTVYGDLELSVIDEMPPGRQSVHTYVLTPQERERAYHLISSQIE
                     AGRQAFVIYPLVEESEQSESKAAVDEHARLQNEIFLNLKLGLLHGRLHPDEKEDVMSR
                     FHSGEFQILVSTSVVEVGVDVPNATVMLIEGANRFGLAQLHQFRGRVGRGGDQAYCLL
                     IPDTPDAAENERLQAMTETDDGFVLAERDLEQRGPGEFLGTRQSGYSELQLANLTDIY
                     LIEKARRQAQVVFEDDPGLQQPAHQLLAAAMESFWSGGKGDIS"
     gene            complement(7458..8288)
                     /locus_tag="AC16CB_10790"
     CDS             complement(7458..8288)
                     /locus_tag="AC16CB_10790"
                     /inference="COORDINATES: protein motif:HMM:NF014679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR3342999.1"
                     /translation="MQPLAIVTDSTSDIPDELIAQHHIHVVPNYVVIDGESAEDGKGM
                     TREAFYTKLPTMISFPTTATASSGTYQQLYNRLLQEGFKQILSIHASSLLSGIFNAAS
                     VAAQPFSKQIHVIDSQYVTLGLGFQVLAAAELALTDPLDNILTVIENVRQRIRVVALL
                     DTLEYVRRSGRVSWARARIGDFLHIKPLVEIRAGRILSLGQVRTRRNGIERLVDMLRN
                     LGNLERLAILHTNAKEEAQRILDSQDLTLSTNPFCVNVTPVIGAHTGPNALGFAAVVR
                     "
     gene            complement(8290..9954)
                     /locus_tag="AC16CB_10795"
     CDS             complement(8290..9954)
                     /locus_tag="AC16CB_10795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HIR3343000.1"
                     /translation="MKPKRLKYFREQPIDGQALKKLIEAGLIWLRTNQQIVNSLNVFP
                     VPDGDTGTNMVLTLQSAMNEISESSESNFGKMAHSVAHGALMGARGNSGVILSQLWRG
                     FARAVDHQEILDATAMAHAFAISRDTAYKGVVRPVEGTILTVSKDIATAAEAALETTN
                     DPFEILEKVVQEASLSVERTPDLLPVLKEAGVVDAGGKGLFFILEGMLRYANGQPLDS
                     ALTTVQPLAAMNLENAIESIEPGQDYEVVVDFRPATALDLESFYSHLEELGTSIQVGE
                     GDGIYRMHIHVPTENLYEPIDYTRSLGVITKVSIENLIDQMQTIEAIRGGSRLTLAPV
                     EPGQIAVVAVSPGPGISRVFASLGAAAIVDGGQTMNPSTQEIINAFENLPTDQVIILP
                     NNKNIILAAKSASELTVKKVAVVPSCTIPQGLAAMLRLAPDGNFDKVAQEMIEALSDV
                     ESGEITTATRSVEIDGVAVREGQIIVLHNGKLVMAADDLEEACLGLLKIAHADHFELI
                     TIFYGADMSKNEVNHIVDAIRRDYPGQEIEVQDGGQPHYQFIIAIE"
     gene            complement(9962..10318)
                     /locus_tag="AC16CB_10800"
     CDS             complement(9962..10318)
                     /locus_tag="AC16CB_10800"
                     /inference="COORDINATES: protein motif:HMM:NF015721.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIR3343001.1"
                     /translation="MTGETNPMGSINVSPRAIASIAYHAARESYGVVGLAHKSLMNGL
                     TQVIVKDPTHGVEVRYDGAQINIDVYVIIEYGTRITTVASSVSNTVRYQVEKALGLPV
                     NDINVHVRGLRVSEID"
     gene            10449..10637
                     /locus_tag="AC16CB_10805"
     CDS             10449..10637
                     /locus_tag="AC16CB_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large ribosomal subunit protein bL28"
                     /protein_id="HIR3343002.1"
                     /translation="MAKCAHCGKTTAFGRNRPWSKKATPRTFRPNLQTISVFENGRKN
                     RKVLCAKCIRTLVKTENE"
     gene            complement(10691..11800)
                     /locus_tag="AC16CB_10810"
     CDS             complement(10691..11800)
                     /locus_tag="AC16CB_10810"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3343003.1"
                     /translation="MPDPILLNNRYQLEERLGYGGMAVVYRAQDLMLERSVAIKILRE
                     NFSGDVDFRERFRQEAKAAANLTHPNIVTIHDFGYDSDRLFIVMEYVPGTHLKSILQR
                     RGRLSVNEAVTLMVQACAGVGYAHRAGLVHCDIKPQNFLITPDKRLKVTDFGIARALA
                     SIQPEEKSEIVWGSPQYFSPEQASGGPPSPASDVYSLGIILYELLTGSIPFLAESPTE
                     LARMHRDDPPPLPRDLNPSISPELEQIMLKVLSKEPSARYRTADQFGRVLVSFSGQNN
                     LATMIVDSEGGSASYAVLPAAEQTASATSPHTPPNQTSRQAYSRPAEPPVTQYTSAQD
                     NPLEVDWITIFLGLIALIFVGGLIPFYLWVYYLFF"
     gene            11913..12863
                     /locus_tag="AC16CB_10815"
     CDS             11913..12863
                     /locus_tag="AC16CB_10815"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR3343004.1"
                     /translation="MPNGMIAYNPNAGRYPSRILTERARDVLREYGWELILEQTTSGD
                     HTTQLAKKAADEGMDAIFIAGGDGSVNRAMRALIGTDTALGVLPCGTANVWAQELGLP
                     GLTWTRWMALEESARRLATAPICAVDIGLCNNRPFLLWAGVGLDAFVVHNLEPRSRWE
                     KHFAVAHYAASIVWHASYWHGVNIRIETDAGRIFGHFLLALVSNVHLYAGGYANLSPH
                     ARLDDGSMDLWLFKGETLGDTVQLVWDLLAGRHIQSERVHQVQFQSLRLESESPTYVQ
                     VDGEPIDVVSPVTITVQPKALRVLVPENTPQPLFNTRAGV"
     gene            12879..13952
                     /gene="cax"
                     /locus_tag="AC16CB_10820"
     CDS             12879..13952
                     /gene="cax"
                     /locus_tag="AC16CB_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015369 - calcium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HIR3343005.1"
                     /translation="MKYIRKDPLILLILAIPFAFLAEFLHWGEVWVFAFSAVGVIPMA
                     RYIGQSTEALAHYTGPKIGGLLNATLGNAAELIITIFAIREGLLDLVKASITGSILGN
                     ILLVLGMSMVVGGKRNGVQSFNRQQATNNAVLLSLAVVALVIPSLFSQSIGPEGSVKV
                     EILSLGVAAVMIVLYVLGLIFSYRLADSLLMAPLGEAGEVTKHTWSLRTAIIVLALST
                     IGVAFLSELLVGSVDPVVSNFGISEFFLGIILIPLIGNVAEHLVAVQVAAENKMDLSV
                     EISLASSLQIALFVAPVLVFVSLLMGNPLTLIFNRFELIALIAGVLIAAVVSADGESN
                     WLEGVELLAVYVILGLAFYLLPV"
     gene            13956..14456
                     /locus_tag="AC16CB_10825"
     CDS             13956..14456
                     /locus_tag="AC16CB_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343006.1"
                     /translation="MDTRALLRYVFSPWVLGGAFLIGVSLLCGSLLFLGATRPGQDAA
                     VPATAIVNVIPLPTATSTVAPPTPSPEITPTSPVPPGPGSGDISIGAFVQVAGTGGDG
                     VRLRLEPGLNGEVRFLGLESEVFLVNNGPQQADGYTWWFLVAPYDESVQGWAVSNYLV
                     LVQNPD"
     gene            14545..14889
                     /locus_tag="AC16CB_10830"
     CDS             14545..14889
                     /locus_tag="AC16CB_10830"
                     /inference="COORDINATES: protein motif:HMM:NF017922.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-butyrobetaine hydroxylase-like
                     domain-containing protein"
                     /protein_id="HIR3343007.1"
                     /translation="MRPTGITANRQTRQLTITWDDGHESVYSFTFVRNACPCAECRGG
                     HEKMGAQLDASVYSLPDEDAPATRLRNLEAVGTYALTFEWEDGHHFGIYNWKYLRTIC
                     PCPVCSSRRIDG"
     gene            14882..15439
                     /locus_tag="AC16CB_10835"
     CDS             14882..15439
                     /locus_tag="AC16CB_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343008.1"
                     /translation="MDEDPIEAVYAFVERAYVRLQASDMLALCLEEGRLSPLQTMVEW
                     LVIAGPQSLTALREILAEVYIRRSQIKNDQHQVFAKLENELRGFGIQLGGIHSLMSLS
                     RLTPVAFLAFLRSQNVMNEGDQLACLQRLSEALDMMNSLDHHLGLLGEIEVYLEDWLW
                     CLIYQSTQQDWGERKMLPSRDRWLL"
     gene            15757..16395
                     /locus_tag="AC16CB_10840"
     CDS             15757..16395
                     /locus_tag="AC16CB_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343009.1"
                     /translation="MEEISEDKDQPTQSPPDKQASKKEGLKFKLGPLEVEGPIGMVGA
                     VLGGLIVGAVVILYLVLVAKQPSSLISAVVGDAVKGTLTAQPTWTPLPTLTPQPVTTV
                     EVIKTVTVIVEKPVEVVKTVEVVRTVEVTVEVPRTVIVEVTPTPLTPPDVLKARLETA
                     FQSASEYLISGDPTRLSDQWLGRLSITEEIKYLRQVFSEITSAQWKVGEYIP"
     gene            complement(16651..>17051)
                     /locus_tag="AC16CB_10845"
     CDS             complement(16651..>17051)
                     /locus_tag="AC16CB_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR3343010.1"
                     /translation="YAEDEMLFSAIRAGASGYVLKQIGAEDLVRAIEAVGRGEALLDP
                     AVTQRIFQEVRKAAKEEEASAFSALTQQERHVLMLVSEGKTNREIAKALFLGEGTVRN
                     YVSSILSKLGVSNRAEAAAYAVEHNLRDYI"
BASE COUNT         3901 a         4126 c         4662 g         4362 t
ORIGIN      
        1 agatttatga ccttacgcat cggcatcttg accgtttctg atcgctcctc ccgaggagag
       61 cgcgctgata cttctggacc agcaatacag gcagtcgttg agcgacaggg gtgggtggtg
      121 tcgaagaccg gcatcattcc cgaccactta gaagccatcc aggagatgct gatcgattgg
      181 gtcgattcgg aggaagtgga cgtcattttg accaccggag ggacaggctt cagtcggcgt
      241 gatgtgacac ccgaagcgac gcttgcgatc attgaacggg cagccccggg gatctctgaa
      301 gccatgcgct ccgccagctt acaggttacg ccgcatgcca tgctctctcg ggctatggca
      361 ggcatccgag ggcgaacgtt ggtgattaat ctgcctggaa gccctaaggg cgcagtggag
      421 aatttgcagg tcgtactgcc tgttttgtca catgccgtgc agctcctgcg cgatgaacca
      481 gatgcagagg caggacacat gcatcatgac gggtgagcaa gcgtgacatt tgtatttggc
      541 gcaagccagc agatacgctt aatctctatg gaaatggttg tataagccag tcaaggggat
      601 cgacctgaat cccgttcacc caaacttccc agtgaagatg ggggccttct acgcgacccg
      661 tgccgcctac caggccaatt tcatccccgc tctcaacccg gtcgccaacc tcaaccataa
      721 tttctgattg gtgcaggtag gctgtgtaca caccccaccc atggtcgatc atggtggcgt
      781 tgccgcgcac agtgagtgga ccagcgaata cgacaacccc gggcgcgggg gcaaagatct
      841 tggtgccggt gcccccggca acatctaatc cggtgtggaa ataggcatat gggcttccat
      901 tataggagcg acgattgcca aagcgagatg tccagcactc attggtttct acgcaataat
      961 cctgggaaag tggcgaaggc atccggaata gtccatccca gaggcgttct ggcgtggcgg
     1021 tagccgctag tgctgaccac tgcgcgtctt cgggacgagt aattgccggg tcaatcgtag
     1081 ccgggtcgac ggtcaaaggc tgatcaaagg ggtaatcctc caatctaacc ccaaccctct
     1141 gagtgaaggt aaaaggcctg tcatcagaaa gcttgccctg caagtggaag ggatagaagg
     1201 ccggttctgt catggcatga atgccgaaca aggctacata tcccccctca gtgtcggaga
     1261 agaagttcag cgggtatccc atgaaggacc cctcgagttc caatccttgc gaagcagcga
     1321 tcttaatgac cccggcttta ccttggcgca aagggagtgg cgttatctct acatcctgga
     1381 ttgctccagg cagtccgcca ggaccagcct ctccatcccc aggtagtaga tagacgtcac
     1441 caggtagcgc cccccaggaa ttcattattt ggttacctct cagaacgctc caggggttgg
     1501 ttccggctaa tatagagagc tccagcaaag attgaccttc agccagagcc attcgtgtga
     1561 ggttggttgc acctgtctct tcttggggga tcaccaggtc tgctcccgca tatagctccg
     1621 acggtgcggt gagatggttt aaacgtgcca gcagggcaat atctatctga tagcgacggc
     1681 tgatactgct caggttttcg ccaaatggga ccgtgaccgt ggttaaaaat ccttgaatcc
     1741 cgtctaaacc cgggataacc agctgggtgc cttccgagag taaattcggg ttggagatgc
     1801 cattcgctaa tgccagttca tctattgtca ggccgaatcg ccgggcaatt ccccccaggg
     1861 tatcgcctgc ctgtacggta taaactggcc cttggggttg ttcctcctgg gcgcgcaccg
     1921 ggaggaccgc aaagaggaga cacatgagta aaaacaggtt aatcctcagt ttcatcttca
     1981 agtgtaactt tgtctccgat agcgaactct gaaaggcgcg agggtgccat ctcaagaata
     2041 tagcgcgctg gctgtttggg tatatagaaa gggcgccacg cccgcgcggt gcataaatcc
     2101 accactacac cttggttgtt gatccatatc actgccaggt ccgtccagac agccagcata
     2161 tggatagctg cgtccaggcg gctatctttt aactggacaa gcagcaaagc ctcatcggag
     2221 gttaaatttc gtcgaaatgt gaagccgcgc aatcggcaca tgaaggatga gcaatacttt
     2281 gcccgaacag gcttgcttgt tggacggctg gtattttgaa tgacaatgtt cttcataagc
     2341 taacttgggg cccgggacgg taatgccgcc tgcagacccc aaagtctatt ataagttgaa
     2401 tggatgtgat gggttgctgt caggacaaga ctctctccac actttctacc aggtcatgcg
     2461 ggctgaatgg tttcgagatg taggcatcga ctttagcgat gtgcaacccc aaaactttat
     2521 caatgctctg agccttagca gtgatgacga tcaccggaat gtcccgcgtg gcttcttctg
     2581 cccgcatttg ttggtagact tcccatccat ccatatccgg catcatcaaa tccaggagga
     2641 ctaagtcagg cagtatttgc cgaactagat ctagcccctc gcgcccaccg ctggctccta
     2701 gcacttcgta gccttttcgg ctgagtatca agcgaacaag ctcgatcatc tcttgctcat
     2761 cttcaatgta aactacttgc ttggggttat ctagcataga cccttccgat ccaggcttgg
     2821 cttgttacgc ataagtttac catatccatc aaaaattgga atatggcata tctacctctg
     2881 cagagtaatc aaaaaaccga aagtccaatc cccctccttt ggggacctgt ccagctcggg
     2941 ttgactctaa agcaaactta tggataatca tttgcaagag gtgcattatg acacgaagtt
     3001 ccccggttct gctcgttact ggcgcatcct ccggtattgg agaggctacg gcgaagcttt
     3061 ttgccgcaca tggctaccgt gttgtactgg ctgcgcggcg gcgcgagcgt ctagaggcgc
     3121 tgggggaaga aatacggaaa atgggtgggg atgcgctacc tgttgttgct gatctgactg
     3181 atttacggca aattgaacag cttgtagata gttctctttc agattatgga aggatcgatg
     3241 tgcttttcaa taatgctggc atgggtcgca tgaactggct tgaaaatctc gacccggttg
     3301 ccgatattga agcgttattt agggtcaacc ttcttggagt ggtgcagaca acacgagcgg
     3361 tgctgcccca catgattatg aggaagagcg ggcatatcat taacatggct tctatggctg
     3421 gttatttggc taccccgacc tactcgattt acgccgccag caaattcgct gtgcgcggct
     3481 tcagtgacgc tttaaggcgt gaagtgggcg tatatggcat acgcgtttcg gggatctacc
     3541 caagtggtgt ggaaacggag tttgcccggc atgcagggat aaagcgcaag accggcatca
     3601 gcacgcctgc gagactgcgg ttgagcgctg agcaggtggc aattagcgtg ttggacgtag
     3661 tacacaggcc gcgacgagcc gtgttgatcc ctgcaccgat gagaatggtg gcatggtttg
     3721 gtttcttttt cccaggattt gtggattgga tcattgaaaa gcgttttacc cgccctgaga
     3781 gaggccttta aacaaattag cgggcttttg gtgcccgcta atcttttttt atgtggtttc
     3841 ttttactctg gttcttgttt ttcactgtgt agtacagaga tcccgttgcg cacctggtta
     3901 aggaagcgct ccaattccat ttccaaacgg cgtaaggtat caagggcgta ctcatccgct
     3961 tctttgcgtg tggattcgcg ttctacctga gccatggcta aaacttcgcc ggctttggca
     4021 tgggcagctt gcactatggc gtcgcgttca accagttgat ccagcttctc gcgagcaatt
     4081 gtgattgtgc ggttggcctc ttcttgcgcc tgcgccaaga tgcggtcgcg ctggttcatt
     4141 acctgctggg cttttttgat ctcttcggga atagccacgc gcatctgatc gatgatatcc
     4201 agcatgcgat cttcatcgat gatgacgtta tgtgtaaatg gaaacggccg gctttgattg
     4261 agcagttctt caaggcggtc aatcagatgc aaaatgtcca caggctgcct ccgcgtgatg
     4321 aaataattat caatagatta cacgcaaata ccgatttcgt caactatccg tgcaaaatgt
     4381 atgccaatct caatcccgca gggaagtggt tggaacaagc tgttgtccat cgcctagctc
     4441 agtaaagcgt gcttgcaagg ccaacttgac atgctcaggc accatgctgc tcacatctcc
     4501 acccagagag gcgatttcgc gcacagtaga tgaagagagg aacgtgtgtt cttcggcggt
     4561 gatcagagcg accagctcaa tatttggagc caggcggtgg tttgcaagtg ccatacggaa
     4621 ctcgtattcg aaatccgaga aaactcgcag gcctcgcaca atcacatgag cgccaatggc
     4681 ttggcagtat tccaccgtaa gcccgctgta tcccatgaca gtaattttag ggttgtcctt
     4741 gaaggattgc tttaccagcg caatccgctc ctcaggcgag aaaagcaatg atttcaaagg
     4801 gcggtcataa accgccacga ccaattcatc gaagagttgt gtggcgcggt ctgcgatatc
     4861 aatgtgtccc aagtggattg ggtcgaaggt acctggaaat actgctcgaa ccattagctg
     4921 atatcccctt ttccgccgct ccaaaagctt tccatggctg cagctagtag ttgatgggcc
     4981 ggctgttgca ggccgggatc gtcttcgaag acgacctgcg cttgccggcg ggctttttca
     5041 atgagataga tgtcggttag attggcgagt tgtaattcgg agtagccaga ctggcgcgtt
     5101 cccaggaatt ctcccgggcc gcgttgctct agatcgcgct ctgcaaggac gaatccatcg
     5161 tcggtctcgg tcatagcctg caggcgctca ttctcagcgg catctggcgt atctgggatg
     5221 agcaggcagt aagcctgatc gcccccgcgt cccacccgcc cgcggaattg gtgcaactgg
     5281 gctaatccga agcggtttgc cccttcgatc agcatcactg tggcattggg cacgtccact
     5341 cctacttcca caacggaagt tgagaccagg atttgaaatt caccgctatg gaagcgagac
     5401 atcacatctt ctttttcatc ggggtgtaag cgtccatgta gaagaccaag cttaagattt
     5461 agaaagatct cattttgcag gcgggcgtgt tcgtccacgg cggccttgct ctcgctctgc
     5521 tcgctctcct caaccaaggg atatatcaca aatgcctggc gaccagcctc gatctggctg
     5581 gagatcaaat gataggcgcg ctctcgttct tgaggggtta gaacataggt gtgcacagac
     5641 tgccggccgg ggggcatctc atcaattaca gatagctcta gatcaccgta gaccgtcagg
     5701 gctagcgagc gagggatagg ggtcgcggtc atcaccagta gatgggggtt ctcccccttg
     5761 gagcgcaggg tggcgcgttg ctctaccccg aaacggtgtt gctcgtcaac gatggctaat
     5821 tgcaggttgg aaaatgttac cgggtcttca agcagggcgt gggtacctat caccaacttt
     5881 atggagccct cggctagacc ttcgcgtatc tcccgcttct cggcttctgg tgtagcacca
     5941 atcattaatc ggatggcttc tggacggata ggatatccgc cttcaggttg ggtagcttgc
     6001 ccatttaccc ccgctagcaa gctcatcatg ctcttatagt gttgaccagc caggatgctg
     6061 gtgggtgaca ttaaagctgc ttgggcaccg agatgagtca caatcgaaat ggctaatgca
     6121 gccacaacag ttttacctga acccacatcg ccttggagaa ggcggttcat gggtatgcct
     6181 gatgccaggt cagtgcgcaa atcattgacc gcgcgctgtt gagcttccgt gagagggaat
     6241 ggcaacccgg ctatctgggt tgccagccaa ttgtcctcaa cttcaaatgc gcgcgccgtg
     6301 cgttcttgcc acatgcgttt ttgacgcagc acgcccagtt gtaacaggaa tatctcgtca
     6361 aatgccagac gatggcgggc agccttcagc aaatcccagt tttctgggaa gtgaatctgc
     6421 tgcaatgcgg tcggtagatc gagcaggtcg gcgttttgac gtatcttttc tgggatatgg
     6481 tctgggatgc ggggcgacca ataggtaatt acttggtcca tcaagcgacg cagccagcgc
     6541 tgggtgatct gagcggtgag cgaatacatt ggcacaatgc gatttgtgct caattgttgt
     6601 tgttccaggg tctcccattc gggatttacc atcaccaaac gtcccaggta ctggtcaatt
     6661 ttgcctgata gggaaatatg cgccccagtt tgcaggcgtt tagccagcca aggattgaac
     6721 caggtaacgc gcagcgcgcc tgtcccatcg ctgatgactg cctcagcgat ctgcgatggt
     6781 cgtcctcgcc gtgggcgaga ggtaatgttc tggaccgtgc cgataactgt cacttcttcg
     6841 ccgtaccaaa gccggttaat cggttttagt tgcgtatagt caagatattt gcgggggaaa
     6901 taatacatca tatcccgcaa ggtatgcatt cccagcctgc ttagggtctg ggcgtgacga
     6961 ggtccgatat tgggtaaaac cgtggtaggc gcgttcaagg cagcggggac ttcagtttca
     7021 accgtggaag ctgtctccga tttatcaatt tgaacctgcg gctggtgctg gggcccatgc
     7081 aggtgaggtg gcagatctat tttggagggg gtaggttccg caggctttgc cggggtagaa
     7141 tctgtttttt cctcttttga cggggtaggc agggtgggta gagagctgct actattctct
     7201 tggacgcgat cccaaattct agtcaggacc tctgcgcgtg cattttcgtc taaacgagag
     7261 tagtctcgca gttgcgagcg gatcgattgg atcaactctt cggaaaggtt gtcgctacgc
     7321 gcttcggctt cccaattatc gagcacgcgt tccaaaccgc ccatcacggc gcggttatcg
     7381 taaccgcgtt cggcttctaa tttgaaaaat ttgtgcaatt tatctaagga tggcttcatt
     7441 aggggctatt ttaccagtta cctgacgacg gctgcaaatc ccagcgcatt tggtccagta
     7501 tgggctccga tcacaggggt gacgttgacg caaaaagggt tagtgcttag ggtaaggtct
     7561 tggctatcta gaattcgctg ggcttcctct tttgcatttg tatgcaagat tgccagacgt
     7621 tccagatttc caaggttgcg tagcatgtcc accagccgtt caattccatt gcgccttgtg
     7681 cgtacttgcc ccaggcttag aatacgacct gctctgattt caaccagtgg cttgatatgt
     7741 aaaaaatctc cgatgcgagc acgcgcccag gagacgcgcc cgctacgccg aacatattcg
     7801 agtgtgtcta gcagtgcaac cacacgaatc cgctggcgaa cgttttctat aacggtcagg
     7861 atattatcca gggggtcagt taaagccaat tcagcggctg ccaatacttg gaatcccagc
     7921 cccagcgtga catactggct gtcgatgacg tgaatttgtt tgctgaaggg ttgcgcggcg
     7981 acactggcgg cgttgaaaat accactcaat aaactggagg cgtgaataga gagaatttgc
     8041 ttgaaacctt cctggagcag gcgattgtag agttgttgat atgttcctga agaagccgtg
     8101 gcagtggtag ggaaactaat cattgtggga agtttcgtgt aaaaggcctc ccgtgtcatg
     8161 cctttgccgt cttctgcact ctctccatca attacaacat agttgggaac aacatgaatg
     8221 tgatgttgag caattaactc gtcgggtata tcggaggtgc tgtctgttac gatggcgaga
     8281 ggttgcatgc tattcaatgg cgatgataaa ctgataatgt ggctgtccgc cgtcttggac
     8341 ttctatctct tgcccaggat aatcacggcg gatggcatcg acgatatgat tgacctcatt
     8401 cttggacatg tcggcgccat agaatatggt gatcagctca aagtgatcag catgcgcaat
     8461 tttcaataat cccaggcaag cctcttccag gtcgtctgca gccatgacca gcttgccatt
     8521 gtgcagcacg atgatttgcc cctctcgtac cgcgacgcca tcgatttcta ccgaacgagt
     8581 ggctgtggta atctcgccac tctctacatc acttaaagct tctatcatct cctgtgctac
     8641 tttatcgaaa tttccatcag gagcgagacg caacatggcg gctagccctt gagggatggt
     8701 gcagctgggt acaaccgcca ccttcttcac cgtgagttca gaagcggatt tggctgccag
     8761 gatgatattt ttattattgg gcagtatgat aacctggtcg gttgggagat tctcaaatgc
     8821 attgataatc tcctgtgtgc tggggttcat tgtctgaccc ccgtccacaa ttgctgctgc
     8881 tcccaggctg gcaaagacac ggctaatgcc gggaccgggt gagacagcaa ctacggcgat
     8941 ctgcccaggc tcgaccgggg ctagtgtaag ccggctaccg ccgcgaatgg cttcaatggt
     9001 ttgcatctgg tcaatgaggt tttcaatgga aactttggtt atgacaccca acgagcgagt
     9061 gtagtcaatc ggctcgtaga gattctctgt aggtacatga atgtgcatgc ggtaaatgcc
     9121 atccccctcg cccacctgga tcgaggtgcc tagttcctct aggtggctgt agaagctttc
     9181 cagatccaag gcggtcgctg gacgaaagtc caccacgacc tcgtaatctt gaccgggctc
     9241 aatagactct attgcgttct caaggttcat cgctgctaaa ggttggacag ttgtcagggc
     9301 gctgtccaaa ggctgcccat tggcgtaacg gagcatgcct tctagaatga agaacaagcc
     9361 tttccctcca gcatcgacta caccagcttc tttaagtact gggagcaggt caggggtacg
     9421 ctccacagac aaactggctt cctgaaccac tttttctagg atttcaaatg gatcgttggt
     9481 ggtctcaagg gctgcttctg cggctgttgc gatatctttg gatactgtca gtatggtgcc
     9541 ttctaccggg cgcacgacac ctttataggc cgtatcgcga gaaatggcaa aagcatgcgc
     9601 catggcggtt gcgtccagga tctcctgatg atctacagcc cgggcaaagc ctctccagag
     9661 ctgtgacaga atcacccctg aattgccgcg cgcccccatc agtgccccat gagcaactga
     9721 gtgtgccatc ttgccaaagt tgctctcgga agattctgaa atttcattca tggcagattg
     9781 cagagttaag accatattgg ttccggtgtc tccatcgggt accggaaata cgttgagtga
     9841 attaacaatc tgctgattcg ttcgcagcca aattagtcca gcctctataa gtttctttag
     9901 agcctgacca tcaataggtt gttcacgaaa atacttcaat cgcttaggtt tcatggcgat
     9961 gttaatcaat ctcactaacc cgtaagccac gcacgtggac gttgatatcg ttcactggga
    10021 gaccgagcgc tttttcaacc tgatatcgta ccgtgttgct gacgctggaa gccacggttg
    10081 tgattcgtgt gccgtattca atgatgacat aaacatcaat gttgatttga gcgccatcat
    10141 agcgcacttc aacgccatgg gttggatctt tgacgataac ctgagtgaga ccgttcatta
    10201 ggctcttgtg agccagaccc accacgccgt atgattcgcg ggcggcatgg taagctattg
    10261 atgcaatggc gcgaggtgag acattgatgc ttcccatggg attagtttcg ccggtcataa
    10321 tgattccttt tcaaattggc ttgtataaag cagcctgatg tggtaagatt gcacggctat
    10381 tgaccgagag gttggagtcc attcgttgag cattggattc acccaaatta taataaggaa
    10441 gtgttattat ggcgaagtgt gcacattgtg gtaaaacaac tgcgtttggc cgcaaccgtc
    10501 cctggtcgaa gaaagcgacc cccaggacgt ttcgaccaaa cttgcagacc ataagcgtct
    10561 tcgagaacgg gcgcaagaac cgcaaggttt tgtgtgccaa atgcatccgc accctggtaa
    10621 agaccgaaaa cgagtgatcg aatagctcct gactttaaaa atcgcggcgc aagccgtgat
    10681 ttttgttttc ttagaagaat agataatata cccataaata aaaggggatc aaccccccca
    10741 cgaaaattaa ggctatcagg cccaaaaaaa tcgtaatcca atcgacctca aggggattat
    10801 cttgagctga agtgtattgt gttactgggg gttcagctgg acgggaatat gcctgccgag
    10861 aggtctggtt aggcggagta tgcggcgatg tagcggatgc ggtttgctct gcggcgggca
    10921 ggacagcgta agaggcagag cctccctccg agtcaacaat catcgtagct aagttgtttt
    10981 gcccgctgaa actgaccaag accctgccaa attggtcggc ggttcgataa cgggctgagg
    11041 gttctttgga gagaaccttg agcataattt gttctagctc tggggagatc gatggattca
    11101 agtcgcgtgg caggggtggc ggatcatccc ggtgcattcg tgccagctcg gttggggatt
    11161 cagccaggaa gggtatgctg ccggtgagaa gctcataaag aatgataccc agagagtaga
    11221 cgtctgaggc aggcgatgga ggtcctccgg aggcttgttc gggggaaaaa tactgcggag
    11281 aaccccagac gatttcgctc ttttcctccg gctggatgga ggccaaggcg cgggcgatgc
    11341 caaaatcggt tactttcaag cgtttatcgg gggttatgag gaaattttgg ggctttatgt
    11401 cacaatgtac cagcccagcc cggtgggcat aaccgacccc cgcgcaggct tgcaccataa
    11461 gggttacagc ctcgttgacc gagagccgac cgcgccgctg caagatagat tttagatggg
    11521 ttcccggcac gtactccatg acaatgaaca gccggtccga atcgtatcca aaatcatgta
    11581 tagtgacgat attgggatgg gtcagatttg cggcagcctt ggcctcctgg cggaaacgct
    11641 cgcggaaatc gacatcgccg ctgaagtttt ccctcaagat ctttatggcg acactacgtt
    11701 ccaacattaa gtcttgggcg cggtatacga ccgccatccc gccatagccc aggcgctctt
    11761 ctaattggta tcggttatta agtaatatgg ggtctggcat taatacatcc acttgccgct
    11821 atcaggtaga attgtaccac cgcaatttct gaagagcaga tattatatcc agtttttcgt
    11881 ttaaaatcat gtaattgtac aggtgttttt gtatgcctaa cggaatgatt gcatataacc
    11941 cgaatgctgg gcgttaccca tcacgaatac tgaccgaacg tgcgcgtgat gtgctccgtg
    12001 agtatgggtg ggagctcatc ctggaacaaa caacaagtgg cgatcatacc actcagcttg
    12061 ctaaaaaagc cgctgacgag ggaatggatg cgatttttat cgctggagga gatggctcgg
    12121 tcaatcgagc aatgagggca ttgattggca cagatacagc tctgggtgtg ctgccttgtg
    12181 gaacagcaaa cgtttgggcg caggaacttg gacttccggg gcttacttgg acacgctgga
    12241 tggctctcga ggagagtgcc aggcgcttag ctactgctcc tatttgtgct gtggatatag
    12301 gtttgtgcaa caaccgaccg tttttattgt gggccggtgt tgggctggat gcttttgtcg
    12361 tccacaatct tgagcctcgt agtcgctggg aaaagcattt cgcggtggcg cactatgccg
    12421 cttcaattgt atggcatgcc agctattggc atggggtgaa tattcggatt gaaaccgatg
    12481 caggtcgaat tttcggtcat ttcctgctgg cattggtgag taatgtgcat ctttacgcgg
    12541 gaggttacgc gaatctgtca ccgcacgcca ggttggacga tggatcgatg gatttgtggc
    12601 tattcaaagg agagacgctg ggtgataccg tccagttagt atgggatttg ctggcgggta
    12661 ggcatattca atctgagcgg gtgcatcagg tacaattcca aagtctcagg ttagaatccg
    12721 agtcgccaac ttatgtccag gtggatggcg agcctatcga tgtggtgagt ccggttacaa
    12781 ttactgtgca acccaaagcg ctacgcgtcc ttgtgcccga gaacacaccc cagccgttgt
    12841 ttaataccag agcaggcgtg taaggtggag tgaacattgt gaaatatatc cgaaaagatc
    12901 cactgatttt attaatctta gccattcctt ttgccttcct tgccgaattt ttgcattggg
    12961 gtgaggtgtg ggtgtttgct ttttccgctg tgggcgtgat cccgatggcg cgctatatcg
    13021 gtcagtctac cgaggcatta gcgcactata cgggtccaaa aattggtgga ttgttgaatg
    13081 ctacccttgg taatgccgcc gagttgatta ttacgatttt cgctatacgg gaaggattgc
    13141 ttgatctggt gaaagcttcg atcacgggat ctatattagg caatatactc ctcgtgcttg
    13201 gaatgtctat ggtggtcggt ggaaaacgca atggagtgca gtcctttaat cgccaacagg
    13261 caaccaataa tgctgtgctg ctctccctag ctgtggtggc cttggtaatt ccgtcattat
    13321 tcagccaatc tattggccca gaggggtcgg taaaggttga aatcctcagc ctcggtgtgg
    13381 ctgcggtgat gatcgtatta tatgtgttgg gattaatatt tagttatcga ctggcagata
    13441 gcctgcttat ggcacccctg ggtgaagctg gggaagtcac caagcacacc tggtcactgc
    13501 gcacagcgat cattgtgctg gctctctcta caattggggt agcattcttg agcgagctgc
    13561 tggtgggttc agtagatccg gttgtgtcca attttggcat ttctgaattt ttcttgggca
    13621 tcattctcat ccccttgatc ggtaacgtgg ctgaacacct ggtggcggtt caggtggctg
    13681 ccgaaaacaa gatggacttg agtgtcgaaa tatcgcttgc ctctagtctt cagattgccc
    13741 tgtttgttgc tccggtgttg gtgttcgtta gcctgctgat gggtaacccg ctaacgctaa
    13801 tatttaatcg ctttgaattg atcgcactca tcgctggtgt gctgatcgcg gctgtcgttt
    13861 cggcggacgg tgagtctaac tggttagaag gagttgaact gctggcggtt tatgtgatat
    13921 tgggcttggc tttctatttg ctgccggttt gatctatgga tactcgcgcc ctcttgcgtt
    13981 acgtatttag tccctgggta ttaggtgggg catttttgat cggggtgtcg ctgttgtgtg
    14041 gctccttgtt attccttggg gcaacgcgcc ctggacaaga tgcggctgtt ccagcaactg
    14101 ctattgtgaa tgttattccg cttcccactg caacctcgac agtagcccca cccacgccct
    14161 cacctgagat cacacctaca tcgccagtcc cgcctggacc cgggtcagga gatatatcca
    14221 ttggagcttt cgtgcaagta gcgggcaccg gtggagatgg agtgcgcttg cgtctcgaac
    14281 cgggattaaa tggtgaagtg cgcttcttgg gcttggaatc tgaggtattt ctagtgaaca
    14341 atgggcctca acaggcagat ggttatacct ggtggttcct ggtagccccg tatgacgaaa
    14401 gcgttcaggg ttgggcagtc tccaattatc tggtgctggt ccagaatcca gattaatccg
    14461 cgctgcttcc attcttgaaa gtttcctgat aatttcccag gttttaaatt gcgttttgag
    14521 ccaattcgat ttatagttac attaatgaga ccgactggaa tcacagctaa tcgacagaca
    14581 cgccagctga ccataacctg ggatgatggt catgaaagtg tatattcgtt tacgttcgtg
    14641 cgaaatgcct gtccatgcgc ggagtgccga ggaggtcatg agaaaatggg agctcagctc
    14701 gatgcgtcgg tttattcact gcctgatgag gatgccccgg caacccgctt acgtaatctg
    14761 gaagccgttg ggacttatgc gctgactttt gaatgggaag atgggcatca ctttggtatc
    14821 tataactgga aatacttgcg tacaatatgt ccatgcccgg tctgcagttc gaggcgaata
    14881 gatggatgag gacccgatag aagctgtata tgctttcgtc gagcgggcat acgttcggct
    14941 gcaggcatct gatatgctcg cgctatgcct tgaagagggc aggctttcgc cattgcagac
    15001 catggtcgag tggcttgtga ttgctgggcc acagagctta actgctttgc gggagatcct
    15061 ggctgaagtg tatatacgcc gttctcaaat aaagaatgac cagcaccagg tctttgctaa
    15121 gctagagaac gagctcagag gctttggcat ccagttgggt gggattcaca gcctgatgtc
    15181 gctatctcgc ctgacacccg tggccttttt ggcgtttctg cgcagccaaa atgtcatgaa
    15241 tgagggcgac caattggcat gcctgcaacg tctttccgaa gcgttagata tgatgaattc
    15301 cttggaccac cacttgggcc ttttaggtga gattgaggtc tatctggagg actggttgtg
    15361 gtgtctcatt taccagtcca cgcaacagga ttggggggag agaaagatgt taccatcacg
    15421 cgatcgctgg cttctataat ctggaaccaa gcagaaattc aattcgttaa tatagtaggg
    15481 tgaaatcgtg gataccctcg cctgggcaat gcccagaccc cccttatctt ccttgacctg
    15541 tccaggtccc aggcctgtta agtgatggtt atattattgg agataggcct gaaagatgat
    15601 agtgacaatt ggtgtacaat cctatcggga gaagaaatta gcatcgatgg agtgtattgt
    15661 tggttcgggc agaaggcgcg gtgatacgtt atccatgatg aagcgggaaa aggtcgtacg
    15721 tgcccgctgt aatgagaaat tgcgagggag ttgaggatgg aagagatcag cgaagataaa
    15781 gaccagccta cccaatcacc tcctgataaa caggctagta agaaagaagg actgaaattt
    15841 aaattaggtc cactcgaggt ggaaggccca atagggatgg tgggtgctgt cttgggcggc
    15901 ttgattgtgg gtgcagtcgt aatattgtat ctcgtcctgg tagctaaaca accatcctcc
    15961 ctaatctccg cagtggtcgg tgatgccgtc aagggtactt taactgcaca acctacgtgg
    16021 actcccctgc caacgttgac accccaacct gtgacaactg ttgaggtaat taaaaccgta
    16081 actgtgattg tagaaaaacc ggtagaggtc gttaagaccg ttgaagtggt gagaactgtc
    16141 gaggtgacag tagaggttcc gcgaactgtt attgttgaag tgactccgac gccgctcacg
    16201 ccgccagatg tgttgaaggc gcgtttggaa acggcctttc aaagtgccag tgagtatttg
    16261 atctctggcg atccgacccg cttgagcgat caatggctgg gtagattaag tattactgag
    16321 gagatcaagt atttacgcca ggtattcagc gagatcactt ctgcccaatg gaaagtgggc
    16381 gagtatatac cctaaaaaaa gaggaagggg tagaagtgta taccttagaa gttaacagca
    16441 atctgatcat cacaggggta tatatatgca gcggtgaatc gatcagggat actgtcgaat
    16501 tgccttatcc tagcctggtt cgagcggaga tacagagttt ggggagagta cgcatctatt
    16561 catggacgaa gagcagcgat tccattcctg gactctgccc aacgcgcagc ccgtgactat
    16621 aaagtatgat aaaagggatt tcgccagata ttaaatgtaa tctctgagat tgtgttccac
    16681 agcgtatgcg gcagcctcag cgcggttgct tacccctaat ttggataata tgctgctgac
    16741 gtaattgcga accgtgccct cacccaggaa cagcgccttg gcgatctcgc ggttcgtttt
    16801 cccttctgaa accagcatta agacgtggcg ctcttgttga gtaagggcgg agaaggcaga
    16861 agcctcttct tcttttgccg ctttgcgcac ttcctgaaaa atgcgctgag taactgccgg
    16921 atcgagcagt gcttcgccgc gacctacagc ctcaatcgcg cgcacaaggt cttcagcccc
    16981 gatctgcttg agcacatatc cagacgcacc agcgcggatc gccgagaaaa gcatctcatc
    17041 ttcggcgtat g
//
LOCUS       DBNGSX010000360         2711 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_655514, whole genome shotgun sequence.
ACCESSION   DBNGSX010000360 DBNGSX010000000
VERSION     DBNGSX010000360.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_655514"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="AC16CB_10850"
     CDS             complement(<1..900)
                     /locus_tag="AC16CB_10850"
                     /inference="COORDINATES: protein motif:HMM:NF042862.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon-like protease helical domain-containing
                     protein"
                     /protein_id="HIR3343011.1"
                     /translation="MTPRKSKSTEPPQPVPPASLSSSALRKLCDPATLSFETTDELPD
                     LQDVIGQPRAIRAIELGSEVAGPGYNTFVLGLSGSGRTTLSREYLERKSANLPIPDDW
                     CYVNNFELPRQPKAIHLPAGRAVEFSDDVQELVKRCQTEISRLFDSEEYTQERDRLVD
                     DIKKKQEAEFMRLQEYVEKYNFAIVRTPFGFVLVPAVNGKPLKSEDIEKLSPEQRTKL
                     SQLQAKLSEEVDKSLDRLRELEKAASLQISDLNERTVMYLLRPLMENLQNKYQGVESV
                     LTHLEAIQKDIVEHSDQFRRSEGS"
     gene            complement(961..1992)
                     /gene="fbp"
                     /locus_tag="AC16CB_10855"
     CDS             complement(961..1992)
                     /gene="fbp"
                     /locus_tag="AC16CB_10855"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HIR3343012.1"
                     /translation="MLSQPDNHSKIITIERHILEEQGFHPEATGVLTNILYDLALAGK
                     FIASNTARGELAKILGSTEDINIQGERVMKLDRLADETIYRLNDHTGRLAVMASEEHQ
                     HILPIPERYPTGKYVLLFDPLDGSSNVDYNASVGTIFSIYRRQSEDGPGTLEDCLQPG
                     RNLVAAGYLLYSISTMLVYTTGSGAHGFTLDPSIGEFILSHENIRIPNTPSYYSVNHG
                     YEKYWSEGVHRFTKQLQGLEGEYESLSLRYIGSLVGDFHRTLLSGGIFYYPADNKDPS
                     KPQGKLRLMYEAAPLAFIMEQAGGYASDGHRNILDIQPDTLHQRTPLFIGNRELVEEA
                     ETCIRIYDT"
     gene            complement(1992..>2711)
                     /locus_tag="AC16CB_10860"
     CDS             complement(1992..>2711)
                     /locus_tag="AC16CB_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343013.1"
                     /translation="KDRWSKFYEPAHTPEEISIRRPFYPARAGNTRQQHLLKQLGFET
                     INDIRRRCELSHPGRRAACPLFWTLGAQQVGKAAISGWRQVLVGENGILPNVSLWPFS
                     DSLTELIQPGQVIVAETYPAEFYTHLGVRFSNRMRGKKTGKRSQVDRAANSSCLLEFA
                     KQAGITLTPSLEIEILDGFGPAPDAEDRFDAIVGLFGMLNVVFGGRSPGEPENEDVQK
                     IEGWILGQEWTDSNSYRNKEE"
BASE COUNT          600 a          671 c          716 g          724 t
ORIGIN      
        1 actgccctcg ctccggcgga attggtcgga atgctcaacg atgtctttct ggatggcttc
       61 caggtgcgtc aagacgcttt ctaccccctg atatttgttt tgcaggttct ccatcaatgg
      121 tctgagcagg tacatcacag tgcgctcgtt caagtcgctg atctgtaggg aggcagcttt
      181 ctccaactcg cgcaagcgat ccaaagattt atccacctct tcgctgagct tggcttgcaa
      241 ttggctcagc ttggtgcgct gctcgggcga gagcttttcg atatcctcag atttaagtgg
      301 cttgccattc acggctggga caagcacgaa gccaaatggt gttcggacga tggcgaaatt
      361 gtatttctcc acgtattctt gcaggcgcat gaattctgct tcttgtttct ttttgatgtc
      421 gtcaacaagg cggtcacgct cctgggtgta ttcttcgcta tcaaagagac gtgagatttc
      481 ggtctggcaa cgcttgacca actcttgaac atcgtcactg aattcaacag ctctacctgc
      541 aggtaggtgg atagcttttg gctgacgtgg caactcgaaa ttgtttacgt agcaccagtc
      601 atcaggtatt ggcagattgg cggatttgcg ttctaaatac tcgcggctta aagttgtgcg
      661 gccggagcca gagagaccta aaacgaaggt gttgtatcct ggtccggcga cctcgctacc
      721 aagctcaatg gcgcgtatgg cgcgcggctg accgatcaca tcctgtaagt ctggcagctc
      781 gtcggtagtt tcaaaggaca gcgtagctgg gtcgcaaagc ttgcgcaaag cactggatga
      841 taagctggct ggggggactg gttgcggcgg ctcagtagat tttgattttc ttggtgtcat
      901 aaacagcctt tttaataagt ctattagata gcgggattaa agtcaatcaa atttctgcct
      961 tcaggtgtcg tatatacgga tacacgtctc tgcttcctcc actaactcgc gattcccgat
     1021 aaaaagcggc gtacgttggt ggagcgtatc cggctggatg tcgagaatat tgcgatgacc
     1081 gtcagaggca tatccccctg cttgctccat gatgaacgcc agcggggcgg cttcgtacat
     1141 gaggcgtaac tttccctgcg gtttggaggg gtctttatta tccgccgggt aatagaaaat
     1201 tcctccagaa agaagtgtgc ggtggaagtc acctactagt gagccgatat agcgtaatga
     1261 gagagattca tactcgcctt ctagcccttg gagctgtttg gtgaacctgt gaacgccttc
     1321 gctccaatat ttctcatagc catgattgac gctgtaatat gagggagtat ttgggatgcg
     1381 gatgttttcg tggctgagga tgaactcgcc aatgcttgga tcgagggtaa agccatgcgc
     1441 acccgaccca gtggtgtaca ccagcatggt gctgatactg taaagcaggt acccagccgc
     1501 caccagattc cttcctggct gaaggcaatc ttccaaggtt ccgggcccat cctcactttg
     1561 gcgccgataa atcgagaaaa tcgtgccaac agaagcattg taatcgacgt tggaagagcc
     1621 atctaaggga tcgaaaagca gaacgtattt tccggtgggg tagcgctctg gtatgggcag
     1681 tatatgctgg tgttcttcgg atgccatgac tgccagacgc ccggtatggt cattgagtcg
     1741 atagatcgtc tcgtctgcca gtcggtccag cttcatcacg cgctcaccct ggatgttgat
     1801 gtcttcggtg ctacccagta ttttcgctag ttcaccacgc gcggtgttgc tggcgatgaa
     1861 tttacctgcc agtgccaggt catacaaaat attggtcaaa acacccgtag cctcggggtg
     1921 gaatccctgc tcttcgagaa tatggcgttc gatggtgata attttggagt ggttgtctgg
     1981 ttgcgatagc attattcctc cttattccgg tagctgtttg aatcagtcca ctcttgccct
     2041 agaatccaac cctcaatctt ttgaacatcc tcattttctg gttcgcccgg ggagcgtccc
     2101 ccaaaaacca catttaacat cccgaaaagc ccgacgatag catcaaaacg atcttccgca
     2161 tcgggggctg gtccgaagcc atctagaatc tctatctcta gggaaggtgt aagggtaatc
     2221 ccggcttgct tggcaaattc taataaacat gatgagttcg ctgcgcgatc tacctgggaa
     2281 cgtttacccg tctttttccc acgcatccgg tttgaaaagc gcacccccag gtgggtataa
     2341 aactcggcag ggtaagtttc tgcgacaatc acttgacctg gctgaatgag ctctgtgagc
     2401 gaatcggaaa agggccagag agatacgttt ggcaaaatgc cattttcccc aaccagcact
     2461 tgcctccacc cggagattgc tgctttacct acctgctgag cacccaatgt ccagaagagc
     2521 gggcaggcag cgcgccgccc tgggtgtgaa agctcacagc gtcggcgtat atcatttatt
     2581 gtttcaaagc ctagttgttt caggaggtgc tgctggcggg tgtttcccgc cctggctggg
     2641 tagaatggcc gtctaatcga aatctcttca ggtgtgtgtg caggctcata gaattttgac
     2701 catcgatctt t
//
LOCUS       DBNGSX010000361         2074 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_932787, whole genome shotgun sequence.
ACCESSION   DBNGSX010000361 DBNGSX010000000
VERSION     DBNGSX010000361.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_932787"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(270..1790)
                     /locus_tag="AC16CB_10865"
                     /pseudo
     CDS             complement(270..1790)
                     /locus_tag="AC16CB_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498465.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
     gene            complement(1883..>2074)
                     /locus_tag="AC16CB_10870"
     CDS             complement(1883..>2074)
                     /locus_tag="AC16CB_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343014.1"
                     /translation="VIHIYSIKSQAKQLIRKHRSGLKNPNNKLREWIMNASSDRDAWQ
                     PSIGYLSLAFARNSEYHRR"
BASE COUNT          437 a          607 c          507 g          523 t
ORIGIN      
        1 atcgatgtgc cggagcgtgg taaacctgag acaaccacca ccgggcgggc cggtgatttc
       61 tttccaaaca gattagaaag gcttttcatc ttttcaggct ggcttgtaat attcgcaagt
      121 cggtttaaaa gttggttgcc agccactttt ccctttaagt tagcacaatt ctatgcacca
      181 gcgcgcacat ctatcctaat ttcaagactg tcctgtttaa agccggtctg gcttatctgt
      241 tgctgcatat catcacgtga ttcttagtct taattgttta acggcactgc cagcagttcc
      301 tcgagagccg ctctagactg ctcctcaatc acagcatgct ggctgctgcc atgcgcgtcc
      361 atggtcacca cagcggggaa atccaccacc tggataaccc acatggcctc gggaacgccg
      421 aattccagct tgtatacccc aatgactttc ttcactgtct gggcgatcag ggaagccgca
      481 ccacccacag catggaaata aacccccggg acctcctggc aagcagccag tgtcttggca
      541 cccatcccgc ctttaccaac cacaccttta atattgaagt ggcgcatcac atcgccttgg
      601 tagggctcct cgcggctgct ggtggtcgga ccggcagcca caaagcggta atccttagtc
      661 tccaacccgg caaccaccgg gccgcagtgg tagatcacgc cacccttgag taagggcttg
      721 atcgcctcat ataccatcag gtcatcttcc gttggctggc gggtcttctt gataaatgtc
      781 tcgatcatcc acttgtgcac gttatctcgg ccggtcacca tcacaccggt gagcaacacg
      841 ggttcgccta ccttgaggct gcggatggtt tcgtcgtcga tgggtagatt aatctggcgc
      901 atatcttcct tcctttatca cgaataactc accaccccgt cctttaagct catccttcgc
      961 ctgcggtatg cccagcacat gtaggacaca gagacaaagt agcttgcggg caagcggttc
     1021 aggctggcga tcttcgtccc cagcacggtc gtgttgccgc cgaaacccat cgggcctatg
     1081 cctaactggt tggcttcacc ggtcaggcgt tcctccaggg cagccagctc tgggttcgga
     1141 ttagcgtcat ctaactggtt gaacagcacc tctttggagc cgtaatacga agagccgcga
     1201 tctcctccaa tagctacacc caggatgccc ggcgcgcagc ctcgtccctg ggctttgtgc
     1261 acagcgtcca aaaccacctt gcgcacacca gccagatcac gacctgctcc taaagcgctg
     1321 ttgggtagcg aatactgcgc cccgacattc tcacaccccc cacctttcaa catcagttcg
     1381 atggtgagag tatccccctc gacctcttcg aaatgaattg ttggaaagtg agaatcgccc
     1441 aggttgttac cagtgttctt cccagtcagg gagtcaaccg agttagggcg caagtaggcc
     1501 cgttgtgtcg cctgctcgac ggcgctttca atctgcgcac gcagcttgcg cgtgctccag
     1561 ccttcaggat ggcgcacgta gaagatcggc gtcccggtgt cctggcatat tggggtggaa
     1621 ttagcccgag agagctccac gtttttgatg atcgtctcca aagcgccgcg tgctgccgag
     1681 cccggctcct ctttctctac cgcctggcgc aacgcgtgtt ctacatcggg gggcaaatcg
     1741 gaagaagtca ggcgcaccaa ttcgaggatt ttttcggtca ggtcttgcat ggttcctcca
     1801 catttcagat gttcgtgggc agtaactggt ggaaatcagg ctttcaggac ctgcccaaca
     1861 tctaagatct gtttttcgtc tatcatcttc tatgatactc cgaattacgg gcaaatgcga
     1921 gtgacaagta acctatgctt ggctgccagg cgtcacgatc tgatgatgca ttcataatcc
     1981 attctcgtag cttgttgtta ggatttttca agccgcttct atgcttgcga atcaattgct
     2041 ttgcctggct tttgatgcta tatatatgaa taac
//
LOCUS       DBNGSX010000362         5938 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_932788, whole genome shotgun sequence.
ACCESSION   DBNGSX010000362 DBNGSX010000000
VERSION     DBNGSX010000362.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5938)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5938)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5938
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_932788"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="AC16CB_10875"
     CDS             complement(<1..474)
                     /locus_tag="AC16CB_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343015.1"
                     /translation="MTKWFKYGLVGILVIGALAVLISGVVMAQDNAFPPPPRPWFGSG
                     RGFEHGIGSGGGLEAVAEALGMTLDELRSQMRDGKTLADLADGKGIDLADLREAVQAA
                     RLEQTRQQIEQAVDDGTLTRDQADWLLEGLDNGYGADFGRGITRVFGKGMAGAVGS"
     gene            684..>5938
                     /locus_tag="AC16CB_10880"
     CDS             684..>5938
                     /locus_tag="AC16CB_10880"
                     /inference="COORDINATES: protein motif:HMM:NF019323.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR3343016.1"
                     /translation="MSDYKSQPTSLPSLSTIRIALFAILIVTLACSLPGLFARPTPTL
                     PAEPTPTFTPAPPTPTPQPLPPAIVESSPPIGGEVPLSGPITLYFNQEMDRQSVESAL
                     SLEPGLTGDFTWHDNATLVFAPAEPLTPDSEITFNLETGARSARGLALTQPISLNFRA
                     VGYLRLVQTLPEPDTSEVDPSSAVVASFNRAVVPLGADPANLSPAFSLQPGANGRGEW
                     VNTSTYIFYPDPPLEGGKAYTVAINPELKGVDTSPLDSAEGWTFYTAPPQLSTFLPDP
                     MGGDIRLDSVITMTFNQPMDDQSVQSGFAMLREGSSPVAGKMTWNEDFTALTFIPDNL
                     LQRSTTYTVKLDGGVQARGGTPLVAGFETSLRSVSPLAVVRSEPSQGGTVPQYNGVAL
                     YLNAPVVGKDIVDHIAVTPSVSNLNAWWDEFSRFISIYGDFAPDTDYTLTLLPSMRDP
                     WGGALAGDYTLNFRTSPLPPSIGLSYGYGSNVLFLTPQDSSLIAQVTNFPNVSTSVGS
                     ASLGEFIAMLGPNSFNYAQSFQAADERTWSQSFDLEPNRNTPVDLYVSPQRNPLPLGL
                     YYLGFNTRSETYFPTKYLIVVGNAHLTFKLSDTQSLVWAVNMDDNTPIQGAMVAIYNE
                     NGEAIAQGVTDSQGIFQSEIPALKDPYTNYYAVLGRPIDGVFGIALSNWNQGVASWDF
                     GLISNYTGPRLKAYLYTDRPLYRPGQTVYFRAILRQAYNGRYTIPDLASLPLTLYKNY
                     SEELISFDLPLSAFGTAHGEYTLPIDAPPGDYRIANSIDDYDISVNFRVAEYRKPEIN
                     LQVEIADEQMKSGDDLVARVNARYFFDAPAGNLPVRWTLYTTPLDFYLPGYQVGVQET
                     RWLEAFYYRPFFDQLGYQIQTGEATTDPQGQLTLELPTEVGETRAKYTLEVTMTDESG
                     LPVSARGSIEANPSSYYIGLRPEAWVGRAGEPAGFDVQVVDWEKKPVGPNDLRAEFQT
                     VEWVEEERPPSESFLGPKFVPRYTPVGSTDFQTNAEGQARLSFVPPEPGTYSLSVYSD
                     EARSQVYIWVSGPGSAVWPNLPNQRLRLVSDRDNYKPGDTAQVFVLNPFPSSAQALVT
                     VERGVVMRSEVRTLQPGSNTLSFPLSSEDAPNIYVAVTLLGLDERGGPDFRQGFINLT
                     VEPSEQTLNVALTSQPQRLGPGEDVTFEVRVTDSAGQPVQGEFSLSVVDMAVLALADP
                     NVPDIASYFYGEQPLGVRTGVALAAYARRFTVNPLGGGGGGGDGMIQVVRERFPDTAY
                     WNAEIVTDGDGRATVSLPLPDSLTTWQVLVRGVADDTRVGQTETQVVVTKDLLVRPVT
                     PRFLLVNDHVQLAAVVQNNTPSDLQAEVSIEATGFTLDNPAVSTQSISVPANGRTRVE
                     WWGITQDVPNVELIFSASARSGDTVYQDATRPALGSLPVLHYISPQTFSTAGILDDSG
                     ERLELVSLPRSFDPQGGELRVELSSSLGGSMITALESLEKNPYECTEQTNSRFLPNLE
                     AYRVLQALNIDSPDLQARLARTLLDGIGRLLARQRPDGGWDWCGGDVSNPYLTAYVLF
                     GLSRAREAGAKISDTPIQRAVEFLTATLYTPDMAAESSQLNRLAFVHFALAYAGSGDL
                     AGQQALYQARDRLSPWAKAVLALAIQRGEPENEAVNTLLSDLSSSAIRSATGAHWEEE
                     QPDVQNMSSPLLTTAMVVYALSQEQPDSPLLAEAVRYLMAHRRVNGTWGSNYANAWIL
                     MALAEVMRSTEEVGASFGFFATLNGV"
BASE COUNT         1247 a         1868 c         1563 g         1260 t
ORIGIN      
        1 gcttccaacc gcgccagcca tgcccttgcc gaagacacgt gtgatgccac gcccaaagtc
       61 agcgccataa ccgttatcca gaccttcgag cagccaatct gcttggtcgc gggtcagcgt
      121 gccatcatcc acagcttgtt cgatctgctg gcgggtttgt tccaaccgcg cagcctggac
      181 tgcttctcga aggtcagcca ggtctatgcc tttaccatcg gccagatcag ccagggtttt
      241 gccatccctc atctggctgc ggagctcatc taaagtcatc cccaatgctt ctgctactgc
      301 ttccaagcct cccccactac ctatgccatg ctcgaagcct ctgcctgagc caaaccaggg
      361 acgtggtggc ggggggaatg cattgtcctg agccataacg actccactga tcagcactgc
      421 cagagcacca ataaccagta ttccaacaag cccgtattta aaccattttg tcatctgtag
      481 cctcctatca caatcaggta atatagttac ccaagtaaaa ccgtcagtcc gtatgcttgc
      541 attcgataac aggatatcat tcagttgttc agaaaatatg tagaagtgat ttggatatga
      601 tttagatcct gtatttgaaa ggtataatga accaattgga acaaaagagc tcctttatcc
      661 gtctcttccg tcaggagaga gctatgtctg actataaatc gcaacctacc agtcttccat
      721 ccctatcgac gattagaatc gctttatttg ctatcctcat cgtcaccctg gcttgctcat
      781 tgcctgggct gttcgcgcgc ccgactccca cactgcctgc tgagcccacg ccgaccttca
      841 cgcctgcccc tcccacgccc actccacagc cgctgcctcc cgccatcgtc gagagcagcc
      901 caccaattgg cggggaagtg ccgctaagcg ggccgatcac cctatacttc aatcaggaga
      961 tggaccgcca atcggtggag agcgcactca gcctggaacc ggggctgact ggtgatttca
     1021 cctggcacga taacgccaca ctggtcttcg cgccagctga gcctctcacc ccagatagcg
     1081 agatcacatt caacctggaa accggtgccc gctcggcgcg cggcctagcc ttgacccaac
     1141 ccatcagctt gaatttccgc gctgtgggtt atctgcgtct ggtgcagaca ctgcccgagc
     1201 cagacaccag cgaggtcgat cccagctctg cggtggtagc gagctttaac cgcgccgtgg
     1261 tgccgcttgg cgccgacccg gcgaatttat cgcccgcctt tagcctgcaa cccggcgcaa
     1321 acggtagggg cgaatgggtc aacacaagca cctatatctt ctacccagat cctcccctgg
     1381 agggcggaaa ggcctacacc gtcgccatca accccgagct gaaaggggtc gataccagcc
     1441 ccctcgattc ggcagagggt tggacgttct acacagcgcc cccacaactg tctacatttc
     1501 tgccagatcc gatgggcggt gacatccgcc tggattctgt gatcaccatg acgttcaacc
     1561 agccgatgga cgatcagagc gtccagtctg gctttgccat gttgcgagag ggctcttcac
     1621 cggtcgccgg aaagatgacc tggaacgagg attttaccgc cctgactttt attcccgata
     1681 acctgctaca acgcagcacg acctacacgg tgaagctgga tgggggcgta caagcccgcg
     1741 gcggcacgcc attagtagct ggcttcgaga cctctctgcg tagcgtctcc ccgctggctg
     1801 tagtgcgaag cgagcccagc caagggggta cagtacccca atataatggt gtcgccctgt
     1861 atctcaacgc gccggtcgtg ggcaaagaca tcgttgatca cattgcagtc accccgtcgg
     1921 tgtccaacct gaacgcctgg tgggatgaat ttagccgttt catcagcatc tatggcgact
     1981 tcgcccccga taccgattac actctcaccc ttctgcccag catgcgcgac ccctggggcg
     2041 gggcgttagc aggcgactat accttgaatt tccgcacatc tcccttgcca ccttcaattg
     2101 gattatctta cggttacggc agcaatgtgc tcttccttac ccctcaggat tccagcctga
     2161 ttgcacaggt aaccaatttc cccaatgtat ccacgtcggt aggcagtgct tctttgggcg
     2221 aattcatcgc catgctgggc ccaaattcat tcaattacgc ccagtctttc caagccgctg
     2281 acgagcgcac ctggtcacaa agctttgatc tagaacccaa ccgcaacacc cccgtagacc
     2341 tatatgtctc gccacaaaga aatccactgc cgcttggatt gtattacctg ggtttcaaca
     2401 ccaggagcga aacctatttc cccacgaaat acttaatagt ggttggcaac gcccacttga
     2461 cctttaagct cagcgacacg caatccctgg tatgggcagt gaacatggac gataacaccc
     2521 ctatccaggg tgcaatggtc gccatctata acgaaaatgg tgaggccatc gctcaaggcg
     2581 tgaccgactc ccagggtatc ttccagtcgg agataccggc cctgaaagat ccctatacca
     2641 actattacgc ggtgctcggt cgcccaatcg atggcgtctt cggcatcgct ctctccaact
     2701 ggaaccaggg tgtggcgagc tgggatttcg gcctgatcag caactatact ggcccaaggc
     2761 tgaaagccta tctatatacc gaccggcccc tctaccgccc aggacagaca gtttacttcc
     2821 gcgccatcct gcgccaggcg tacaacgggc gctataccat ccccgacttg gccagcctgc
     2881 cgctcaccct gtataaaaac tactccgaag agctaatcag ctttgatctg ccactctccg
     2941 ccttcggcac cgcccacggc gaatataccc tgcccatcga tgcccccccc ggtgattacc
     3001 gcatcgccaa ctccatcgac gattatgata tctcggtcaa cttccgggtg gccgaatacc
     3061 gtaaaccgga gatcaacctg caagtagaaa ttgcggatga gcaaatgaaa tctggcgatg
     3121 atttggtggc tcgtgtcaat gcccgctact tcttcgacgc gcctgccggt aacctgccgg
     3181 tgaggtggac cctgtatact acgcctttag acttctacct ccccggttat caggtcggcg
     3241 tgcaagaaac cagatggtta gaggccttct actatcgacc atttttcgac cagttgggct
     3301 accaaattca aaccggtgaa gcgaccaccg acccgcaagg ccaattaacc ctggagttac
     3361 ccactgaggt gggtgaaacg cgtgccaagt acacattgga agttactatg acagatgaga
     3421 gcgggttgcc agtcagcgct cgcggcagca tcgaggccaa cccgagttcg tactatatcg
     3481 gcctgcgccc agaagcctgg gtagggcgtg ctggggaacc cgctggattc gatgtacagg
     3541 tggtagattg ggagaagaaa cctgttggcc caaatgacct gcgcgccgaa tttcaaacgg
     3601 tcgagtgggt cgaggaggag cgccccccca gcgagagctt ccttgggccc aagttcgtgc
     3661 caaggtatac ccctgttggc agcaccgact ttcagaccaa cgccgagggg caggcgcgcc
     3721 tgtctttcgt gccacccgag ccgggcacct atagcctcag cgtttacagc gatgaggcgc
     3781 gcagccaggt atatatatgg gtaagcggac ctggcagcgc cgtctggccg aacctgccca
     3841 accagcgcct gcgtcttgtt tccgaccggg acaattacaa acccggcgat actgcccagg
     3901 tatttgtgct caaccccttc ccctctagcg ctcaagcact ggtgaccgtt gaacgcggtg
     3961 tggtgatgcg ctccgaagtg cgcaccctgc agcccggcag caacaccttg agcttccccc
     4021 tcagctctga agatgccccc aatatctatg tcgccgtaac gctactcggt ttagatgagc
     4081 gcggggggcc ggattttcgt cagggtttta taaacctgac cgttgaacct tctgagcaga
     4141 ccttgaatgt agccttgacc agccagcctc agcgccttgg accgggcgaa gacgtgacct
     4201 ttgaagtgcg tgtcaccgat tctgcaggcc agcccgttca gggtgagttt tccctctcag
     4261 tagtggacat ggccgtactg gctctggccg acccaaacgt gcccgacatt gcctcctact
     4321 tctacggcga gcagcccctg ggcgtgcgca ccggggtggc actggctgcc tacgctcgcc
     4381 gctttaccgt aaatcctctc ggcgggggtg gcggaggtgg agatgggatg atacaggttg
     4441 tgcgcgagcg tttccctgat accgcctact ggaacgctga gatcgtcacc gacggagacg
     4501 gtcgcgccac agtcagcttg ccgcttcccg acagcctgac cacatggcag gtattggtgc
     4561 ggggcgtggc tgacgacacc cgcgtcggtc agactgagac gcaggtggtc gtcaccaaag
     4621 acttgttggt tcgtccggtc acgccgcgct tcctgctcgt gaacgaccac gtccaactgg
     4681 cagccgttgt gcaaaacaac accccaagtg acctgcaagc cgaagtctcc atcgaagcta
     4741 ctggcttcac cctagacaat ccggcagttt ccacacagtc catctccgtt cctgccaatg
     4801 ggcgcaccag ggtggagtgg tggggtatca cccaggatgt gccgaatgtg gaattgatct
     4861 tctcagcctc tgcccgctcc ggcgacactg tatatcaaga cgcaacccgg cctgcccttg
     4921 gcagcctgcc agtcctgcac tatatctccc cacaaacctt tagcaccgca ggcatcctgg
     4981 atgatagcgg cgagcgcttg gagctggtta gcttgccgcg ctccttcgac ccgcagggtg
     5041 gggagctgcg cgtggagctt tcatcttcct tgggcgggtc gatgataacc gccctagaat
     5101 ctctggagaa aaatccctac gagtgcaccg agcagaccaa ctcgcgcttt ctgcccaacc
     5161 tggaggctta ccgtgtcctg caagcgctca acatcgattc gcctgacttg caagcccgcc
     5221 tggcacgcac cctgctggat ggcatcggcc ggctgctggc acgccaacgt ccggatggcg
     5281 gctgggattg gtgcggcggg gatgtcagca atccatacct gaccgcttac gtgctctttg
     5341 ggttgagccg cgcccgtgaa gcgggggcaa agattagcga cacccccatc cagcgggcag
     5401 ttgaattcct gaccgccaca ctctacacgc cggatatggc tgctgagagc tcgcaactca
     5461 accgactggc tttcgtgcac tttgcactag cctatgctgg gtcgggtgac ctggctggcc
     5521 aacaggctct ctaccaggct cgcgaccggc tcagcccctg ggccaaggca gtgctggcac
     5581 tcgccattca gcgcggggag ccggagaatg aggctgtgaa cacattgctc tctgacctga
     5641 gctccagtgc aatccgctcg gccacaggcg cgcattggga ggaagagcag cccgacgtgc
     5701 agaatatgag ctcgccgctt ctgaccaccg caatggttgt ctatgcgctg tcacaagagc
     5761 agccagactc gcccctgcta gccgaggccg tacgctactt gatggcccac cgcagggtaa
     5821 acggcacctg gggctccaat tatgcaaacg cctggatttt gatggctctg gcagaggtga
     5881 tgcgctccac cgaagaagtg ggtgccagct tcggcttttt cgccactttg aatggcgt
//
LOCUS       DBNGSX010000363        18451 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1763331, whole genome shotgun sequence.
ACCESSION   DBNGSX010000363 DBNGSX010000000
VERSION     DBNGSX010000363.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 18451)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18451)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18451
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1763331"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..1126)
                     /locus_tag="AC16CB_10885"
     CDS             complement(17..1126)
                     /locus_tag="AC16CB_10885"
                     /inference="COORDINATES: protein motif:HMM:NF047050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6544 family protein"
                     /protein_id="HIR3343017.1"
                     /translation="MLRILTIVLAVLTGIHGLIHLMGFAAYWPLAKINELPYKTSLLA
                     GRLEVGMAGMRVFSFLWLLATLGFVIAAIALALGKSNWAPLMLGAALLSIVLCTLDWS
                     AAFRGALIDVFFLLVLAMVFGLRMQPSPFPSYTGVPAPLITSPIPAGLPEPVERFYRM
                     HHGDEVPEYHSVVVSMRGTLRFMGITFPARLRFSHIAGQDYRHYIEATFYGYPVMKVN
                     EHYLGGHSRLELPFGVVENDPKVDSAANQGLWAETFAFFPAVILTDGRVRWEPVDENT
                     AKAYVPFGESEQVFTVQFDPQTGYLRGMETLRYRDQKVGTIRWRTELMRGENSNEPIQ
                     ISATWEDEATPWMVVEIEETVFNTDLTEYILQTGQ"
     gene            complement(1128..1754)
                     /locus_tag="AC16CB_10890"
     CDS             complement(1128..1754)
                     /locus_tag="AC16CB_10890"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3343018.1"
                     /translation="MSPNTNPEREPGDTRQRIIQAAIQLFGKQGYSQTSTRAIAEAAG
                     VNEVTLFRQFGRKKDLLIACIEAHNAAGFSATFESELTGDYAEDILRMAELQIRDTTA
                     NLEILRLLSCDAHNMPELRQAMLAGGRGNLARLSRYFQRQIGRGVVRADLPAEVLASA
                     FDSLFSSNIVFEYLFQGNLSPRLTTNEVIRPLAALFVRGTQAIDQRSV"
     gene            1931..4015
                     /locus_tag="AC16CB_10895"
     CDS             1931..4015
                     /locus_tag="AC16CB_10895"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HIR3343019.1"
                     /translation="MDKKAQDQSMLASLELLYDVSREIAAALDLRTVLHRVLFLSMKN
                     VRAISGSIIVLDGNQQPVESAIITGSQLHEQTTQQLRITYERGLAGWVIRNQKAVLVP
                     DTSQDERWLRRPDDDAERTGPKSAVSAPILARDHLVGVMTLVHPTPGFFTQDHLALVR
                     AITDQAGVAVLNARLYAESQRQTRAMTALAESAATITASLKTDDVLQQILEQISQALQ
                     VEVVSLALIDPQTQELVFRAASGEKGPNVIGIRLAPGEGIAGWVAREGIGIIVPDVRQ
                     DPRFLPTVDKLLNFVTKAIACAPIRLRGEVIGILQAINPKDGAFDPDALQVITGIGGL
                     AGTAIHHAQLFERMEAAHQRYRELFEDSIDPILITDLEGRILEANRQVAMITGMNRES
                     LLNAMIDHFQIVDQAELGEKFEKLASGETTSYETCLKAEGGREVPIQVYVRNVFIDGI
                     SNLQWILRDVTERKNLDNLRNDLISMIYHDLRSPLSNIVSSLDVIQALMPQDSDPAVE
                     PLLDIAIRATERIERLTNSLLDIRRLEAGQSIASRELVSPTSITKEAVKNVLPIVENK
                     NQEITLLIEENLPRVWVDTDMIGRVLTNLLENAVKHTPAGSTIYLGARLEGNQVIMWV
                     QDNGPGIPPDERERIFDKFSRLHGRGGPKGLGLGLAFCRLAVEAHGGQIWVDDGPEGG
                     SNFKFTLPTQMD"
     gene            4127..5002
                     /locus_tag="AC16CB_10900"
     CDS             4127..5002
                     /locus_tag="AC16CB_10900"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3343020.1"
                     /translation="MHQLKPGIFYEDAYLGVTLGALIFSQGTIMIDAPLRTEDTRAWR
                     SALITLRGGSSRLLISLDAHPDRTLGTRAMECTVISHHKTAQVFRNRPVIFKGQSAEG
                     GADWETYDDVIGTRWISPDITFSEKMILYWGGPEVILEHHPGPASGSIWVVIPQAGVL
                     FVGDAVTPNQPPFLSQADLPIWIETLDHLENTFKGYTIVSGRGGLVEPDAVRAQQRYL
                     KNIYKGLERLAKRNALPEATENLINSLLKEVDYPDKFSDQYTQRLRSGLFQYYIHHYR
                     QETLPDQLDFEDSDS"
     gene            5018..6073
                     /locus_tag="AC16CB_10905"
     CDS             5018..6073
                     /locus_tag="AC16CB_10905"
                     /inference="COORDINATES: protein motif:HMM:NF017864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIR3343021.1"
                     /translation="MHAAHYLPLLELTRGSIVESIHFGAIAVVDARGNLIASYGDPQA
                     VSYLRSTAKPFQALPFIENDGHKFYKLSQREIAVMCASHSGTNEQVAVVQGLQKKTGV
                     SEADLQCGVHPLSHEPTLEAIRQRGEQVTPNRNNCSGKHTGMLAYARMKALPTEDYLN
                     PHHPIQQDILNTFAAMCKVTVEQIEIGTDGCSAPNFAVPLFNAALGLGRLCDPETGGI
                     LPPSRQVACHTILAAMTSHPEMIGGPDSFDTRLMQATGGRILAKSGAEGYFGLGLRPG
                     TLSPNSPGAGIAIKVSDGDARQRARPAVVLEVLHQLGVLESSEMDRLSEYGPSTGVYN
                     WQRLEVGRLRPGCQLSR"
     gene            6076..6747
                     /locus_tag="AC16CB_10910"
     CDS             6076..6747
                     /locus_tag="AC16CB_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HIR3343022.1"
                     /translation="MDQASRALAVHQRLIKRYGHPQWRNHLPPLDELVSTILSQNTND
                     LNRDRAFEALRRQFPSWEQVRDAPTDEIIQAIRPAGLANQKGPRIQQVLREITLQRGS
                     LSLDFLNDLSPAEASAWLMSFKGVGPKTAAIVLLFSLGKPAFPVDTHIYRVSGRIGLR
                     PAKMNAEQAHEHLAELLPPETYYAAHLNLIRLGRETCQARRPNCPTCPIQDLCDYYAQ
                     LSSAA"
     gene            6865..7461
                     /locus_tag="AC16CB_10915"
     CDS             6865..7461
                     /locus_tag="AC16CB_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343023.1"
                     /translation="MKARQAGSRHLLLLYRRWMDRLWRSTLILGLLLFGIWFWTWWYG
                     TNLLHGQEDLWLLLGAVVLLGFTLFAFFARGMAYVQPRRDHLRLVTPFLRTNISYRRL
                     VSTHPADFTQIFPIKNASWAQRRLLEPFYGMTAVVVELNNYPLPPVLLRLFLAPQMFY
                     RRAKGFVLLVPEWMALSTELDSLVGAWQQEQKRRQLVR"
     gene            complement(7605..9503)
                     /locus_tag="AC16CB_10920"
     CDS             complement(7605..9503)
                     /locus_tag="AC16CB_10920"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR3343024.1"
                     /translation="MVRAKTRRAYGFWDSPLTPKRMAGGLHLNDARWDESGALVWLEG
                     RSERAALVVQAPGVGAPRDLNSEYSVRAKVGYGGGDFGVGHGQVYFVESTSGRIYRQS
                     LEYGLAQPVTPAFGHAAAPTPSPDGDWLLFVHTYEGQDSLGIAGTQGRFWPGRLVSGD
                     DFYMQPTWHPDGKQVAWIAWNHPNMPWDGTTLWLGNLTFPPNGLPLMGEAKPLAGDEN
                     TAIFQPEFSPDGRTLAYVSDASGWWQIYLYDLESGATRQLTDEQAEHGAPAWVQGMRT
                     YAFGPGGVSLYFIRNQAGFASLWKADVASGRLSKLELPAQYTWLEKIAVSPVDGQIAL
                     IASGGDTPPRLIVQHADGGTHLLRHSQAEDVPVSAYTLPQAITWQGMDDGTIHGLYYP
                     PHSETFEGIGLPPLMVLIHGGPTSQRMASFDEEVQFFTSRGYAVLQVNYRGSTGYGRL
                     YRNMLRGNWGIYDVQDAVSGARYLVEQGRVEGSRMVIMGGSAGGYTVLLALEQFPGFF
                     KAAVNLYGVTNQFDFVAGGTHKFEERYNDTLLGPYPEAGQVYRERSPIFFVDKIQDPV
                     AVFQGEDDNVVPRRHSDELVKSLQRRGVPHIYHVYPGEGHGFSKPETIEHLYQELDKF
                     LKQYVLLT"
     gene            complement(9549..10244)
                     /locus_tag="AC16CB_10925"
     CDS             complement(9549..10244)
                     /locus_tag="AC16CB_10925"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR3343025.1"
                     /translation="MTPRFDVSLLPPAPNFDFEIALWERGCCYVAGVDEAGRGALAGP
                     VAAGVVMFPDDPGLLPELVGVRDSKQMTHSERAMWAERLKQLALTWAVGFASVEEIDS
                     LGIVPATRLAARRATRALGEQPEHLLVDFLELPELPIPQTPLVKGDRRSLSIAAASIL
                     AKTERDGLLCELEARYPGYGLAQHKGYATQAHRDALRRLGPAPIHRHSFSPVSSLSPL
                     APLPGGSGEKTKT"
     gene            complement(10228..10959)
                     /locus_tag="AC16CB_10930"
     CDS             complement(10228..10959)
                     /locus_tag="AC16CB_10930"
                     /EC_number="2.4.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004581 - dolichyl-phosphate
                     beta-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HIR3343026.1"
                     /translation="MTGPFLSIVIPAYNEEKRLPKTLEQVRSFLESQPYTAEVLVVEN
                     GSQDRTFEVAQDFARRHPGVSVLQETGRGKGRAVRRGMLAARGEYRFMCDADLSMPVE
                     EINYFLPPALQDFDIAIGSREAPGAIRYNEPHYRHLGGRAVNTMIRLLALPGLQDTQC
                     GFKCFRAPIAEELYRLQTLTGWSFDIELLYIARQRGYRIVEIPVQWYFNPESKLNVVQ
                     DAIKMGLDIFTIHLNNMKGVYDAKV"
     gene            complement(10976..11806)
                     /locus_tag="AC16CB_10935"
     CDS             complement(10976..11806)
                     /locus_tag="AC16CB_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343027.1"
                     /translation="MKVIDNPEFREKPGTPAKLDSVLDSLGMGRSGSPELQSQEDLIA
                     SLEKTLDNRFVLLRNVTLEDLDTPIPLVLLGPSGVSVIFPSPARGVFRAKEETLEYMD
                     EYNRVYRQANPNLLARTIMMAKSAEAYLNSNFPAPIEVEPVLYFSDPGTHVESVRPSL
                     RILLVDGLERFIAGQLQAAVLYEREEVQKLVDLLLKASRITERQVSPYPERDAFSFVD
                     ESGLDKPSIADRLPRGEGIVSALNKIPFSGRQWLLLGCMVVVNVVVLIAFVLLVLFTS
                     "
     gene            complement(11835..12626)
                     /locus_tag="AC16CB_10940"
     CDS             complement(11835..12626)
                     /locus_tag="AC16CB_10940"
                     /inference="COORDINATES: protein motif:HMM:NF013078.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HIR3343028.1"
                     /translation="MQLAYNLSVPTPFYHLSVAEELLGQPRLGKQARLLLEAQRSAFL
                     FGNTAPDVQVISHQPRQDTHFFDLPLRSLAPSPWELLLRDYPSLARADLLPPAQAAFL
                     AGYLCHLQADWLWVKDIFVPVFGLHSTWGSFPQRLYLHNVLRAYLDRHILPSLANGAC
                     TSLAEARPAGWLPFVHERYLYEWRDFLVTQLQPQASVQTVEVFAARQGISPSEFYHLL
                     DSEEAMEREVFARIPRQALENYREILIAENAKLIHNYIARATAIP"
     gene            12987..15332
                     /locus_tag="AC16CB_10945"
     CDS             12987..15332
                     /locus_tag="AC16CB_10945"
                     /inference="COORDINATES: protein motif:HMM:NF013132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR3343029.1"
                     /translation="MTVEIILVLAILAIAILLFVTERVRMDVVAFLVLGGLAVAGLVT
                     PAQALSGFSNPAVVTVWAILILSGGLARTGVASLIGRYMLRLAGDSEVRLLLFLSITA
                     GILSGFINTVGVVSLFLPVVIDIARRTKRPPSKILMPLAFASLLGGLNTLIGTPPNIL
                     ISEALREAGLRPFQMFDYTPVGLSALLAGTVYMILVGRHLLPNLDLARDFSAERARDF
                     KSLYDLHEHMVILHMPEGSILNGKTLAESRLGSALGLNVVAIIRQGQIMRSPQPSFAL
                     KSGDRLLVEGRLEQFSELRGKNHIILDQEGFSVERLVSADVQLVEARLSPSSSLIGQT
                     LRDIGFRHSYAVIVLAILREGVPFRTNLENIPLLGDDILLLQGRRQHIEELKHDTDFA
                     ISTPSSLQDFKLEERMMVVQVPPNSSLVGKSLVESHLGDSYGLGVLGIMRSGETNLMP
                     SPDERLQENDVLIVKGRYSDLHLVEGLQSLEVENGPPPDLGELESEEIGLVEAVLSPH
                     STLAGKTLRELNFRFKYGLTVLAIWREGRAYRTNLRDTELRFGDALLLYGPRERLRML
                     GSEPDFLVLTEEAQEPPRREKIPAALFIMALVLIPVVFGWLPIAISAILGVALMILTN
                     CLTMEEAYRYIEWKAVFLIAGLLPMGIAMEQTGAAEILANGMVTLIGNMGPMVLMAGF
                     FVVAALASQVMPNPAVAVLLAPIALRTASDLGVSPYPLMMTVAVSASAAFLSPVGHAA
                     NLLVMGPGGYRFADYIKVGFLLTIVTMIVAILVIPILWPFS"
     gene            15349..17133
                     /locus_tag="AC16CB_10950"
     CDS             15349..17133
                     /locus_tag="AC16CB_10950"
                     /inference="COORDINATES: protein motif:HMM:NF015557.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR3343030.1"
                     /translation="MTPEIALTLAIVLGALVLFGTDKVRVDLVALLVLLTIGLTGLVS
                     PKEVFAGFSNSAVITVWAVYIVSGGLFNTGVADTLGKFILRLAGKNEMRLIAVIMLTC
                     GILSAFMNNVGATAMLLPAVVSISRQNKISVSKLLIPLSFSSLLGGKLTLIGTPANIL
                     ATSIVAENGLPTFSFFDFTSLGIVVFVTGILYMVFIGRHLLPIRQSPSEQQIPQQLKA
                     YVSEVRVLPKGKLVGHTLLESRLGADYDLTVIAVVREEKTLAAVDRNTVIQPDDILLI
                     EGSVESLMRATTDIGLALTSHPHMDLDNIDNSNLEIFEAILAHYSTMVGHNLRELQFR
                     ERYGFTVLAIRRHGELITKRLRDISLKFGDDFLLQGTRDRLPVLQEGNDFLTLEPIKI
                     PQDHRKRMPIAIGLMLLVIGLVLFAKLDIAIAMVIGAVGMVLTRCLTMDEAYQNIDWR
                     TVFLVAGMLPLGMAMETSGTARYIADLFLGMTGGLGPLAALAGMYILAALVTQPMSNA
                     AAMVLVVPIALDTALELGANHLTFTLAVVIGAATSFLTPVGHKANVLVFGPGGYKFFD
                     YTRVGFLLTIFLFIATMIALPILFPLFP"
     gene            17451..>18451
                     /locus_tag="AC16CB_10955"
     CDS             17451..>18451
                     /locus_tag="AC16CB_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343031.1"
                     /translation="MPFRSCWWAMLLIGLLVVTTTAGAQTTASAILFPPQTQSFPRIT
                     TFMDVHDAQTSFVHGVQASEVQIIENGRSLPVVELRELRPGVQIVLALNPGPPFGIRD
                     SQGISRYEFIRDALLDWAKVRRGSSIDDLSLLISAGPEISHVSDTTELVSALGSYQPD
                     SDNASPGLEILLRAIDIAADATPRAGMERAVLLVTPPLEGDISFGLQELISRAALLRV
                     HVFVWLVASQQAFESQPAILLSRLSEQTGGQFFTFSGIEDIPNPEGYFEELRDIYQIT
                     YDSKITQGGSQELAVEIRHGEQVITSPSLTFDFELSPPDPAFIFPSLEISRSIPAERR
                     "
BASE COUNT         4063 a         5407 c         4837 g         4144 t
ORIGIN      
        1 gaaaaccccg attcgatcac tgaccggtct gaaggatgta ttcggttaaa tcggtgttga
       61 ataccgtctc ttcgatttca accaccatcc agggggtagc ttcatcctcc caggtggctg
      121 agatttgaat cggctcattt gaattctccc cccgcatcag ctctgtccgc cagcggattg
      181 tgccgacttt ctgatctcga tagcgtaggg tttccatccc gcgcaggtat ccagtctgcg
      241 ggtcgaactg taccgtgaag acctgttcgc tttccccaaa gggcacatag gcttttgcag
      301 tgttctcatc cacgggctcc cagcgcacgc gcccatcggt gagtattacc gccggaaaaa
      361 atgcaaaggt ttccgcccat aagccctggt ttgctgcgct gtccaccttg ggatcatttt
      421 ccactacccc aaagggcaat tcaagccggc tgtgaccgcc caggtagtgc tcgttcactt
      481 tcatcactgg gtaaccgtaa aaggtggctt cgatgtaatg gcggtaatcc tgaccggcta
      541 tgtggctgaa acgtaatcga gctgggaagg tgattcccat gaagcgcagc gtgccgcgca
      601 tgctgacgac cacagaatgg tactctggca cttcatcgcc atgatgcata cggtaaaagc
      661 gctccacagg ttctggtaac ccagcaggaa tggggcttgt tatcagcgga gcgggtacgc
      721 cggtatatga cggaaaggga gacggttgca tgcgcaggcc gaacaccatc gccagcacca
      781 gcaagaaaaa gacatcgatc agcgcgccgc ggaaggctgc actccaatcc aacgtacaca
      841 gcacgatcga taaaagggct gcgcccagca tcagcggcgc ccaattcgat ttaccaaggg
      901 ctagtgcaat agctgcgatc acaaagccaa gcgtcgccag caaccacaag aagctgaaaa
      961 cccgcattcc cgccatgccc acctccagcc gtcctgccag caagctggtt ttatagggaa
     1021 gctcgttgat tttcgccaat ggccagtaag ctgcgaagcc catcaggtgg atcaggccgt
     1081 ggattccggt caagactgcc aacacgatcg taagaatccg taacatctca aacgctcctt
     1141 tgatcaatcg cctgtgtgcc gcgcacaaac agcgctgcaa gcgggcggat aacttcattc
     1201 gttgtcagcc tgggtgaaag attgccttga aacaagtatt caaaaactat gttggaggaa
     1261 aacaggctat cgaacgcaga tgccagcact tccgctggca ggtctgcccg gactaccccg
     1321 cgaccgatct gccgctggaa atagcgactc aggcgggcca ggttgccgcg cccacctgcc
     1381 agcatcgcct ggcgtagctc cggcatattg tgcgcgtcac aagacagcag gcgcaggatt
     1441 tccaggtttg ccgtcgtatc ccgaatttgt aactccgcca tgcgcaagat atcctctgca
     1501 taatccccag tcagttccga ttcaaacgtt gcggaaaaac cggcagcgtt atgggcttcg
     1561 atgcaggcga tcaacagatc cttcttccgt ccgaattggc ggaagagggt gacctcgttg
     1621 accccagcgg cttcggcaat agcgcgtgtg ctggtctggg agtagccctg tttcccaaac
     1681 agctgaatag ctgcctggat gatacgctgt cgtgtgtcgc caggttctct ctccgggttg
     1741 gtattgggtg acataattca ttctcctgta ttgacatgca agtgagcact tgcatcatat
     1801 tataccctgc tgcttagtgc aagcaagtga ttacttgcat attttgggga tgacattcat
     1861 cacccgggaa ccaactttct caagtattgc aaatgtgcta tactcaaacg caaatacgag
     1921 accctgattg atggataaaa aagcacaaga ccagagtatg ctagcctctc tcgagctgct
     1981 ctacgatgtc agccgagaga ttgcagctgc cttagacctg cgcaccgtac tccaccgcgt
     2041 gctcttcctg tccatgaaga acgtgagggc aatcagcggc agcatcatcg tgctggacgg
     2101 caaccagcaa ccagttgagt ctgccatcat caccggctcg caattacacg aacaaaccac
     2161 ccaacaactg cgcatcacct acgagcgcgg cttagccggt tgggtgattc gcaatcagaa
     2221 agctgttctg gtgccagata ccagccaaga cgagcgctgg ctgcgccgtc cggatgacga
     2281 cgccgaacgc accggtccaa aatccgctgt cagcgcgccc atactggcgc gggatcatct
     2341 ggtgggggtg atgaccctgg tgcaccccac cccgggtttc ttcacccagg atcacctggc
     2401 tctggtgcga gctattaccg accaggcggg cgtcgctgtg ctcaatgccc gcctgtatgc
     2461 agagagccag cgccagacgc gcgccatgac cgccctggca gagagcgctg ccaccatcac
     2521 ggcatcctta aaaaccgatg atgtcctgca gcaaatcctt gagcagatca gccaagctct
     2581 acaggtagaa gtggtctccc tggcattgat cgatccgcaa acccaggagt tggtcttccg
     2641 cgctgcgtct ggtgagaaag gcccaaatgt tatcggcatt cgcctggctc caggtgaggg
     2701 tattgccggt tgggtggcaa gagaaggcat cggaataatc gtgcccgatg tgcgccaaga
     2761 tccgcgcttt ctccctactg tcgacaaact actcaatttt gtcaccaaag ccatcgcctg
     2821 tgcacccatc cgcttgcgag gcgaggtgat tggcatcctg caagccatca acccgaaaga
     2881 tggcgcattc gacccggacg ccctgcaggt tataacaggt atcggcggat tggctggcac
     2941 tgccatccac cacgcccagc tcttcgaacg catggaagct gcccaccagc gataccgcga
     3001 attatttgaa gacagcatcg acccaatttt gatcactgat ttggaagggc gtatcctgga
     3061 ggctaaccgc caggttgcca tgatcaccgg tatgaatcgg gagtcgctac tcaacgccat
     3121 gatcgaccat tttcaaatcg tcgatcaggc tgagctcggc gagaaattcg aaaaactcgc
     3181 ctctggcgaa accaccagct atgaaacttg cctaaaggca gaaggcggtc gcgaagtccc
     3241 tatccaagtc tatgttcgca acgtcttcat tgatggtatc tcaaacctcc aatggatttt
     3301 acgggacgtt accgagcgta aaaatctgga taacctgcgt aacgatctca tctccatgat
     3361 ttatcacgat ctacgctcgc cactgtccaa catcgtctcc agcctggatg tcatccaggc
     3421 attgatgccc caagattccg atcccgcagt tgagcccttg ctggatatcg ccatccgcgc
     3481 cactgagcgt atcgagcgcc tgaccaattc gttgctggac atccgccgcc tggaggcagg
     3541 tcagtccatt gccagccgcg agctcgtttc ccccactagc atcaccaaag aagcagttaa
     3601 aaacgtgcta cccatcgtgg agaacaagaa tcaggagatc acgttgctga tcgaagagaa
     3661 tctaccgaga gtctgggtag ataccgatat gatcggacgc gtgctgacaa acctattgga
     3721 gaatgctgtc aagcacacac ctgctgggag caccatctac ctgggcgctc gcctggaagg
     3781 caaccaagtg ataatgtggg tgcaagataa cggtccaggc attccgcccg atgaacgcga
     3841 gcgcattttc gataaatttt cccgtctgca cggacggggt ggtccaaagg gattaggttt
     3901 ggggttggcc ttctgccgat tagctgtgga ggcccacggt gggcagattt gggtggatga
     3961 cggaccagaa ggtggttcga actttaaatt caccctaccc actcagatgg attgagcgat
     4021 ggcgagggtt tgctctgatc gagttcggga tatgcgggat ttactcccag attaggaatt
     4081 cctcccgctc aagccggatg gcccttcgat ataatattgg agatatatgc accagcttaa
     4141 accaggaata ttctacgaag atgcttatct gggtgtgaca ttgggcgcct tgatcttctc
     4201 tcaagggacg atcatgatcg acgccccgct acgcaccgaa gacacgcgtg cctggcgctc
     4261 ggctttgatc accctacgag gtggatcttc ccgcttgctg atctcgctag atgcccaccc
     4321 agatcgcact ctgggcacgc gtgccatgga atgtaccgta atctcgcacc ataagaccgc
     4381 ccaggtcttc cgcaaccgcc cggtgatctt caaggggcag agcgcagaag gcggtgctga
     4441 ttgggagacc tacgatgatg ttattggcac acgctggata tctcctgata tcaccttctc
     4501 tgaaaagatg atcctgtact ggggtggacc ggaggtcata ctggagcatc atcccggccc
     4561 ggcgtcgggc tccatctggg tggtcatccc ccaggctggt gtgctcttcg taggcgacgc
     4621 ggtaacaccc aaccagcctc ccttcctctc ccaagccgac cttcctattt ggatcgaaac
     4681 gctggaccac ctggaaaata ctttcaaggg ctacacgatc gtcagcgggc gtgggggact
     4741 ggttgagcca gatgcagtgc gagcccagca acgctatcta aagaatatct ataaagggct
     4801 ggagcgccta gctaaacgca atgctctacc cgaagcaaca gaaaatctga tcaactcact
     4861 tcttaaagag gtagattatc cggacaagtt cagcgaccag tacacgcaac gcttacgctc
     4921 tggtttgttc caatattaca tccaccacta ccgccaggag acgctgcctg accagctcga
     4981 tttcgaagac agcgatagtt aatataatag acagcttatg catgcagcgc actacttgcc
     5041 cttgttggaa ctaacccgcg gctcgatcgt tgaatcgatc cacttcggtg ccattgccgt
     5101 ggtggacgcc cggggaaact tgatcgccag ctacggcgac ccgcaggcgg tctcctacct
     5161 gcgctcgact gccaaaccct tccaagcgct gccgttcatt gagaacgacg ggcacaaatt
     5221 ctataaactg agccagcgag aaatcgctgt gatgtgcgcc tcgcactcgg gcacgaacga
     5281 gcaggttgcc gtggtacagg gcttgcagaa gaagaccggg gtcagcgagg ctgatctgca
     5341 atgtggcgtt cacccactaa gccatgagcc taccctcgag gcgatacgcc agcgaggtga
     5401 acaggtcacc cccaaccgca acaactgctc cggcaaacat accggcatgc tggcctacgc
     5461 ccgcatgaag gctttgccca cagaagatta tctcaacccc caccacccta tccagcaaga
     5521 tatcctgaac acattcgctg ccatgtgcaa agtgacggtt gaacagattg agatcggcac
     5581 cgacggctgc tccgccccaa actttgctgt gccgctcttc aatgctgccc ttgggctggg
     5641 ccgcttgtgc gacccggaga ccggcgggat actcccaccg tctcgccaag ttgcatgcca
     5701 tactatccta gcggcgatga catcccaccc cgagatgatc ggcgggccag acagcttcga
     5761 tacccgcctg atgcaagcca caggcggccg catcctggca aagtctggtg cggaaggcta
     5821 ctttggtctt ggcctgcgtc ctggaacgct cagcccaaac tcgccgggag caggcatagc
     5881 catcaaagtc tccgatggag acgcgcgcca gcgagcgaga cccgcggttg tccttgaagt
     5941 cctgcaccag ctgggcgtgc tagagtcatc ggaaatggat cggctttcag aatatgggcc
     6001 aagcaccgga gtgtacaact ggcaaaggct tgaagtcggt cggttgcgtc cgggctgtca
     6061 gctaagcagg tgagcatgga tcaagccagc cgcgccctcg ctgtccatca aaggctcatc
     6121 aagcgctatg gtcatccgca atggcgcaac cacctgccgc cgctagacga gctggtctcc
     6181 accatcctct cccaaaacac caatgatctc aaccgagacc gcgcctttga ggcgctgcgc
     6241 cgccagttcc cctcctggga gcaggtgcgc gacgccccaa cagatgaaat tattcaggcc
     6301 atccgcccgg cgggtctagc caaccagaag gggccgcgca tccagcaggt gctgcgagag
     6361 atcacccttc agcgaggcag cctcagcctg gattttttaa atgacctatc ccctgccgag
     6421 gccagcgcct ggttgatgag cttcaagggc gtgggaccga aaacagcagc catcgtgctg
     6481 ctcttctcct tgggcaagcc agccttcccg gtggatacgc acatctaccg ggtgagcggg
     6541 cgcatcggcc tgcgcccagc caagatgaat gccgaacaag ctcacgagca cctggctgag
     6601 ctgctcccgc cagagaccta ctacgctgcc catcttaatc tgatccggct gggccgtgag
     6661 acctgccagg cgcgccgccc caattgcccc acctgcccca ttcaggatct atgtgattac
     6721 tatgcgcagc tttcatcagc cgcttaattt attaaaccca aggggaagaa gagccgctcg
     6781 cacttcatgg tctacgcaaa atgaccgagg gatgattgcc acgcctctag gggaaggggg
     6841 cagggtgatg ggagaaagat acctatgaaa gctcgtcaag ctggctcgcg ccacctcttg
     6901 ctgctctaca ggcgctggat ggaccgcctg tggagatcga ccctgatcct gggtctgctg
     6961 ctcttcggca tctggttttg gacgtggtgg tatggcacca acctgctcca cgggcaagaa
     7021 gacttgtggt tgctgttggg agcggttgtc ctgcttggct ttaccttgtt tgccttcttc
     7081 gcccgcggta tggcttatgt ccagccgaga cgcgaccatt tgcggctggt cacccccttc
     7141 ctgcgcacca acatctccta ccgccgcctg gtcagcaccc atccagcgga tttcacccag
     7201 atcttcccaa ttaagaacgc cagctgggcc cagcgccgcc tgctcgagcc attctacggc
     7261 atgaccgccg ttgtagtgga gctgaataat tacccgctgc cccccgtctt gctgcgctta
     7321 ttcctcgccc cgcaaatgtt ctaccggcgc gctaagggct tcgtgctgct ggtgcccgag
     7381 tggatggctc tcagcactga gctagactcg ctggtaggag cctggcagca ggagcagaag
     7441 aggcgccagc tagttcggtg aagcattgct aaactcccac ccagtatctt cttctattct
     7501 ctattcccaa attatttggg gcggcgcggc ttgcacgcaa tcaacctata tttaagaaga
     7561 agtgctcctc ccatgaacat atgctccacc tgaaccccca tccttcatgt caggagcaca
     7621 tattgcttga gaaacttatc caactcctga tataaatgct cgatcgtctc aggtttcgag
     7681 aacccgtgcc cttcgcctgg gtagacatgg tatatgtgcg gcacgccacg ccgctgcaat
     7741 gacttgacta attcatccga gtggcggcgc ggtaccacgt tgtcgtcctc cccctggaag
     7801 accgccacgg ggtcttgaat cttgtccacg aagaagatcg gcgagcgctc gcgataaacc
     7861 tgccccgcct cgggataggg acccagcaag gtatcgttgt agcgctcctc gaatttgtgt
     7921 gtgcctccgg caacgaagtc gaactggttt gtaacgccgt agagatttac ggctgctttg
     7981 aagaaacccg ggaattgctc taacgccagc agcacggtat agccacccgc actgcccccc
     8041 atgatgacca ttcggctacc ctctaccctg ccctgctcca caagatagcg cgccccgctg
     8101 accgcatcct gaacatcgta aatgccccaa ttgccgcgca gcatgtttcg atacaaacga
     8161 ccataaccag tgctgccccg gtagttcacc tgaagcacgg cgtagcctcg gctggtgaag
     8221 aactgcacct cctcatcgaa actagccatg cgctggctgg ttgggccacc atggatcagc
     8281 accatcaatg ggggcaagcc gattccttca aacgtctcgc tgtgtggcgg gtaataaaga
     8341 ccatgtatcg taccgtcatc cataccctgc caggtgatcg cctgcggcag agtgtaagcc
     8401 gatacgggca cgtcctcagc ctgcgagtgg cggagcaggt gcgtgcctcc atcagcatgc
     8461 tggacaatca gccgcggggg tgtatctcca cctgaggcaa tcaaagcaat ctgcccatcc
     8521 actggggaga ctgcgatctt ctccagccag gtgtactgtg cgggtagctc cagctttgaa
     8581 agccgcccgg aggccacatc tgccttccac aagctggcaa atccagcctg gttgcggata
     8641 aagtacaggc tcacccctcc cgggccaaaa gcataggtgc gcatcccctg cacccaagct
     8701 ggcgcgccgt gctccgcctg ctcatccgtc agttggcgag tcgcaccact ctccaggtcg
     8761 taaagataga tttgccacca accactggca tcagagacgt aagccagggt gcgcccatcg
     8821 ggcgagaatt ccggctggaa aatggcagtg ttctcatccc cagccagggg tttcgcctcg
     8881 cccatcaaag gcaagccgtt tggtggaaag gtcaggttgc ccagccacaa ggtcgtgcca
     8941 tcccagggca tgttcggatg gttccaggca atccaggcca cctgtttgcc gtctggatgc
     9001 caggtgggct gcatgtagaa atcgtcgcca gagaccagcc tgcccggcca gaacctgccc
     9061 tgcgtgccag cgatgcccag gctatcctgg ccctcatagg tgtggacgaa gagcagccaa
     9121 tcgccatccg gtgagggggt tggggcagcc gcgtgcccga aagccggagt tactggttgg
     9181 gccagcccgt attccagaga ctggcggtag atgcgcccgg aggtagactc tacaaaatag
     9241 acctggccat gacccacgcc aaaatcgcca ccaccgtagc ccaccttcgc ccgcactgag
     9301 tattcactgt ttaaatcgcg gggcgcaccg actccaggcg cttggacgac cagggctgcc
     9361 cgctcggagc gcccttccag ccacaccagc gcaccgctct catcccagcg ggcatcattg
     9421 aggtgcaggc cgcctgccat gcgcttcggc gtaagcgggc tgtcccaaaa accataggcg
     9481 cgccgggttt ttgctcgtac cataatgcct ccgtatgtga tgtttccggc taattcgcag
     9541 ccgggaaatc acgtttttgt cttttctccg cttccgccgg gaaggggagc aagcggggaa
     9601 agggatgaaa cagggctgaa actgtgtcgg tggatcggcg cggggcccag gcgcctcaat
     9661 gcatctcggt gcgcctgggt ggcgtagccc ttgtgctgcg ccaagccata gccggggtag
     9721 cgtgcctcca gctcacacag caacccatcc cgctccgtct tggccaggat agaagctgca
     9781 gcgatgctca acgagcgcct atcccctttg accagtgggg tctgcgggat cggcagctcg
     9841 ggcagctcca ggaaatccac cagcaggtgc tcaggctgtt cacccagggc gcgcgtggcg
     9901 cggcgagccg ccaggcgagt ggcaggcaca attcccaggc tatcgatctc ctccaccgag
     9961 gcgaagccca cagcccacgt cagggctagc tgcttaagtc gctcagccca catggcgcgc
    10021 tccgaatggg tcatctgctt ggagtcgcgc acgccgacca actcgggtag cagaccagga
    10081 tcgtcaggaa acataacaac gcctgccgca accgggccag ccagcgctcc gcgcccggct
    10141 tcatccacgc ccgccacgta gcagcagccc ctttcccaca gggcgatttc gaaatcgaag
    10201 ttcggcgcag gcggcagcag agatacgtca aaccttggcg tcataaacgc ccttcatatt
    10261 attcagatgg atggtgaaaa tgtccagccc catttttatg gcatcctgca caacgttgag
    10321 cttactctca ggattaaaat accattggac aggaatttct acaatgcgat aaccgcgctg
    10381 gcgggcgata tacaagagct cgatgtcaaa cgaccaacca gtgagggtct gtagtcgata
    10441 gagctcctct gcaatcgggg cgcgaaaaca tttgaagccg cactgcgtat cttgcagtcc
    10501 gggcagcgcc agcaagcgga tcatggtatt gaccgcccgg cctcccaggt gacggtaatg
    10561 cggttcgtta tagcgaatcg caccaggcgc ctcgcgcgag ccaatggcga tatcaaaatc
    10621 ttgcaaggca ggtggtaaga aataattgat ctcttccacc ggcatcgaca ggtcagcatc
    10681 gcacataaaa cgatattcac cgcgcgccgc cagcatacct cgtcgcacgg cgcgcccttt
    10741 gccccgcccg gtttcctgca ggacacttac tcctgggtgc cgccgcgcga agtcttgcgc
    10801 cacctcaaat gtccggtcct gactgccatt ttccacgacc agtacttcag cggtatatgg
    10861 ctggctttct aagaacgagc gcacttgctc cagagttttt ggcaggcgtt tttcttcgtt
    10921 ataagccggg atgacaatgg agagaaaagg tccggtcaat gatatagacc tctaatcagg
    10981 aagtgaacaa caccagcaac acgaacgcaa tcaagacgac gacattgacc acgaccatac
    11041 atcctagcaa aagccactgg cgaccggaaa aggggatttt gttgagggct gagacgatcc
    11101 cctcgccacg cggcagccga tcggcgatcg agggcttatc caatccgctt tcatcgacga
    11161 aggaaaaggc atcccgttct ggataaggcg atacttggcg ctcggtaatc ctgctggctt
    11221 tcaggagaag atccaccagt ttctgaacct cttccctctc atataaaaca gcagcctgca
    11281 actgtccagc gatgaaacgc tccagcccgt ccaccaacag gatgcgcagg gacggtctca
    11341 ccgattcgac gtgcgtcccc ggatcagaga aatacaacac cggctcgacc tcaatcgggg
    11401 cagggaagtt gctgtttaaa taggcctccg ctgacttagc catcatgatg gtgcgcgcca
    11461 gcaggttcgg atttgcctgc cgatagaccc ggttatattc atccatgtat tccagggttt
    11521 cttccttagc cctgaacacg cctcgcgctg ggctagggaa gatgacgctc acgcccgacg
    11581 gccccaacag caccagcggg atgggggtat ccaggtcttc tagagtgaca ttgcgtaata
    11641 acacaaagcg gttatccagg gtcttttcca aagaggcgat caaatcctcc tgcgactgca
    11701 gctcaggtga cccactcctt cccatcccca gcgagtcaag cacagaatcg agcttggcag
    11761 gggtaccggg cttctctcta aactcaggat tatcaataac tttcatttga taagtctcct
    11821 atcgataatg catcttatgg tatcgccgtg gcacgggcaa tgtaattatg aatgagcttg
    11881 gcattctcag cgatcaaaat ctcgcgataa ttctctaggg cttgtcgcgg gatgcgggcg
    11941 aagacttccc gttccattgc ctcttctgag tcgagcaggt ggtaaaactc gctaggagaa
    12001 atgccttgac gggcggcaaa cacctcgaca gtctggaccg aagcttgtgg ctggagctgg
    12061 gttactagga agtcgcgcca ttcgtataag taccgctcat ggacaaaagg cagccatcca
    12121 gcaggtctgg cttcagccag gctggtacat gccccatttg caaggctggg caggatatgc
    12181 ctatccaggt aagcccgcaa gacgttatgc agataaagtc gctgggggaa actgccccag
    12241 gtactgtgca atccaaagac gggcacgaaa atatccttca cccacagcca gtcagcttgt
    12301 aaatggcaaa gatacccagc taaaaatgct gcctgcgcag gaggcaatag atccgccctg
    12361 gccagcgaag ggtaatcacg gagcaacagc tcccagggcg atggtgctag cgagcgaagc
    12421 ggcaggtcaa aaaaatgcgt atcctgcctc ggttggtgag agatcacctg cacatctggc
    12481 gcggtatttc cgaacaggaa cgcgctgcgc tgagcctcca gcaggaggcg cgcctgtttg
    12541 cccaagcgcg gctgcccgag caattcctcg gctacactga gatggtagaa tggcgtcggc
    12601 acactcaaat tataagccag ttgcattggg ataaacaaac aataatgtga agccgatgct
    12661 gtaccacatc ttacctcaca aatacttgcc acaacccttt gtggtagggc aatcgcatta
    12721 ggacgatttc ccaccactga agagagtgtt tgggggattg cgcatgcccc tcagggcata
    12781 tgcaattgcc ctactctttg tggccatcca ccacgcatat atcctttacc atgtataatt
    12841 tctaaccaac ccggaactat ttcaaaaccg cttgattgca gcacatgcgc ttattatgct
    12901 taatgggata cagtttcagg caaaaatcgt aatacatagc ttgctcgtaa cggcaagtaa
    12961 gtgcatagcg accagttgga tatgccatga cggttgaaat catccttgtc ttagccatct
    13021 tagcaattgc catccttctc tttgtcaccg aacgggtgcg catggacgtg gttgcctttc
    13081 tggtgcttgg aggtctggcg gtggcgggtc tcgtcacccc agcgcaggcg ctctccggtt
    13141 ttagcaaccc tgcagtggtt acagtttggg cgatcctgat ccttagtggc ggattggctc
    13201 gcactggggt ggcaagcttg attggacgct atatgctgcg cttagcggga gatagcgaag
    13261 tccgcttatt gctcttcctt agcataactg ctggcatcct ctctggtttt ataaacacgg
    13321 ttggtgtcgt ctccttgttc ttgccggtgg taatcgatat cgcccgccgc acgaaacggc
    13381 caccttctaa aatcctcatg cctctagctt ttgcctcgct gctaggcggc ttgaataccc
    13441 taatcggcac accaccaaat atcctgatca gtgaagcctt gcgggaagct ggcttgcgtc
    13501 cgttccagat gtttgattac accccagttg gtttatcagc cctgctggca ggcacggtat
    13561 atatgatcct ggtagggcgc cacctattgc caaacctaga ccttgcccgc gacttctccg
    13621 ctgagcgagc cagggatttc aaatcgctat acgatttgca cgagcacatg gttatccttc
    13681 atatgccaga gggctctatt ttgaatggaa agaccctcgc tgagagccgc ctaggctcgg
    13741 cgcttggact caacgtggtg gcaatcatcc gccaagggca aatcatgcgc tctccccaac
    13801 cttcctttgc cttgaagtcc ggcgaccgcc tgttggttga agggcgtttg gagcaatttt
    13861 ccgagttgcg gggaaagaat cacattatct tggatcaaga aggcttttct gtcgagcgcc
    13921 tggtttcggc agatgtgcag ttagttgaag cccgactctc ccccagttcc agcttgatcg
    13981 gccaaaccct gagggatatc ggattccgac acagctatgc cgtcatcgtt ctggcaattt
    14041 tgcgcgaggg cgtgcctttc cgaaccaatt tggaaaatat tccattgctt ggtgacgaca
    14101 tactgctgct tcagggtcgc cgccaacata tcgaagaatt aaagcacgac actgattttg
    14161 cgatctctac tccgagctca ctccaggatt tcaagctcga agaacgcatg atggttgttc
    14221 aagtgccccc taattcatcc ttggttggca aatcgctggt cgaaagccac ctcggcgatt
    14281 cttatggact gggtgtgctg ggcatcatgc gtagcggaga gaccaacctg atgccatctc
    14341 ccgatgaaag gctgcaggag aatgatgtct tgatagtgaa aggcagatac agcgatttac
    14401 atctggtcga aggcttgcaa agcttggagg ttgaaaatgg ccctccgccc gacctgggag
    14461 aactagagtc ggaggaaata gggctggtcg aggccgtcct ctctccgcac tcaaccctag
    14521 ctgggaaaac cttgcgcgaa ttgaacttcc gctttaaata cgggctcacc gtccttgcca
    14581 tatggagaga ggggcgtgcc tatcgcacta atttgcggga taccgagcta cgctttggcg
    14641 atgcgctctt gttatatgga ccgcgtgagc gcttgcgcat gttgggcagc gaacctgatt
    14701 tcttggtcct caccgaagaa gcccaggagc cgccccgccg agaaaaaatc cctgccgccc
    14761 tgtttattat ggcgcttgtc ctgattcctg tcgtctttgg ctggctgccg attgcaatct
    14821 cagccatcct cggcgttgcc cttatgatac tgaccaattg cctcacaatg gaagaggcct
    14881 atcgctacat cgagtggaaa gcggtctttc tcattgcagg cttacttccc atgggcattg
    14941 ccatggaaca gactggcgca gcagagatat tggctaatgg tatggtaaca ttgatcggta
    15001 atatgggtcc tatggtattg atggctggtt tctttgtggt ggcagcctta gcctctcagg
    15061 taatgcccaa tccagcagtt gcggtcttat tggcacccat tgccctgcgt actgcaagcg
    15121 acctgggcgt atcgccttac cccctgatga tgacggttgc cgtatccgca tccgccgctt
    15181 ttctcagccc ggtaggtcac gcggctaacc tgctggtcat gggaccaggc ggttatcgct
    15241 tcgcagatta catcaaagtt ggatttttac ttactatcgt tacgatgatc gttgctatat
    15301 tggtgattcc cattctatgg cctttctcat gacttatagg actaacagat gacccctgaa
    15361 attgctctca ctctggcaat tgtgcttgga gctctggtgc tcttcggcac cgacaaagtg
    15421 cgcgtcgacc tggtcgcctt gctggtgctc ctcactatcg gtcttacagg gctggtcagc
    15481 cccaaagagg tttttgctgg tttttccaat tccgcggtta tcacggtctg ggcggtctat
    15541 attgtctcgg gcggtctctt caacaccgga gttgctgata ctctggggaa gttcatcttg
    15601 cgtctggcgg gtaaaaacga aatgcgcctg attgccgtca ttatgctcac ctgcggcatc
    15661 ctctccgcct ttatgaataa cgtcggcgca accgccatgt tgctgcccgc tgtcgtcagc
    15721 atctctcgcc agaacaagat ctcggtctcc aagctcctca taccgctctc gttctcctca
    15781 ctcttaggtg gcaaattaac cttgatcggt acacccgcta acatcttagc gacaagcatc
    15841 gtcgccgaaa atggcctgcc gaccttcagc ttttttgact tcacctcgct cgggattgtc
    15901 gtcttcgtta ctggcatcct gtatatggtt ttcatcggtc gtcatctttt gcccatccgc
    15961 caatccccta gcgagcagca gatcccgcaa cagttgaaag cctatgtgag cgaagtgcgt
    16021 gttctcccaa aaggcaaact ggttggtcac acacttctgg aatccagact cggtgctgac
    16081 tatgatctca ctgtgattgc tgttgtgcgt gaggaaaaaa ccttggctgc cgtcgaccgt
    16141 aacacggtta tccagccgga tgacattctt ttgatcgaag gctcggtaga aagcctgatg
    16201 cgcgccacaa cggatatcgg tctagccttg acaagccatc cccatatgga tttggacaac
    16261 atcgacaact ccaaccttga gattttcgag gccatcttgg ctcattattc aaccatggtt
    16321 ggccataacc tgcgcgagct ccaatttcgt gaacgctatg gattcaccgt cctggctatc
    16381 cgccgccatg gagagctaat caccaagcgc ttgcgcgaca tcagcttgaa atttggtgac
    16441 gactttttac tccagggtac ccgcgatcgt ttgccagttt tacaggaggg aaatgacttc
    16501 ctcacccttg agcctatcaa aatcccgcaa gaccaccgca agcgcatgcc catcgccatc
    16561 ggcttgatgc ttcttgtgat tggcctggtt ctctttgcca aactggatat tgccatcgcc
    16621 atggtcatcg gcgcagttgg catggtgctc acccgctgcc tcaccatgga cgaggcgtat
    16681 caaaacatcg actggcgtac cgtcttccta gttgctggca tgctacctct tggcatggcg
    16741 atggagacct ccggcacagc ccgttatatt gcagatctct tccttggcat gaccggtggc
    16801 ttgggtcccc tcgctgctct agcagggatg tatatcctcg ccgccctcgt cacccagccc
    16861 atgtccaatg cagctgccat ggttctcgtc gtccctatcg ctctagacac cgccctggag
    16921 ctgggtgcca accatctcac ctttacattg gcagtcgtca tcggcgcagc taccagcttt
    16981 ctcaccccgg ttgggcataa agccaacgtt cttgtctttg gtcccggcgg atacaaattc
    17041 tttgactaca cccgcgttgg cttcctgctc actattttct tgttcatcgc caccatgatt
    17101 gccttaccca tccttttccc ccttttcccc tgagagatcg cctttcaatc ggtggttcaa
    17161 tcagccccgc agtgtattct tgtcaaccat tctttcgtca ttttctaaaa atcgttggca
    17221 atctaccagg tggaatttca gtactgcggg cttatagcgc cgatcagggc aatttcatac
    17281 gccctgaggg gcatgcgcaa tcccgtaaaa agagtatttg gggtgttttg gtgggggcat
    17341 acccccacca aaacacccca ttttctctct tcagtggtgg gaaattgccc aaatgcgatt
    17401 gccctatagc gccgattttt taggctgcga tcttgtgcta aaattggcat atgcctttcc
    17461 gttcgtgctg gtgggctatg ctgctgatcg ggttgctcgt tgttacaacg actgccggag
    17521 cgcagactac cgccagcgct attctattcc ctccacaaac ccagtccttt ccgcgcatca
    17581 ccaccttcat ggacgtgcat gatgctcaga cgagctttgt gcacggcgtc caggcctctg
    17641 aggtgcaaat tatcgagaat gggcgctcgc tacccgtggt ggaattacgt gagctgcgcc
    17701 caggtgtgca aatcgttttg gctcttaacc ctggtcctcc ctttggcatc cgagatagcc
    17761 aagggatatc aaggtatgag ttcatccgag atgcgctgtt agattgggca aaggtgcgcc
    17821 gcggctccag tatcgacgac ttaagcctgc tgatctccgc tggacccgag atcagccacg
    17881 tctctgacac aacggaattg gtctctgcgc ttggaagcta ccagccagat tcagacaacg
    17941 catcgcccgg gctggagatc ctgctgcgcg ctatcgatat tgctgctgat gccacaccaa
    18001 gagcggggat ggaacgtgct gtcctgctgg taacgccgcc cctggagggc gatatctcgt
    18061 ttggcttgca ggagctaatt tcccgggctg ccctcctgcg cgtgcatgta ttcgtctggc
    18121 tggtcgcctc tcagcaggca ttcgagagcc agccggcaat cctgctttcc cgcctgtcgg
    18181 aacagacagg ggggcaattt ttcactttct caggtattga agacattccc aacccagaag
    18241 gttatttcga agaactgagg gatatctacc agataactta tgactccaag atcacacaag
    18301 gcggtagcca ggaactggcg gtggaaattc gccatggcga acaggtgatc acttctccct
    18361 ccttgacctt cgatttcgaa ctgagccctc ccgatcccgc ctttattttc ccatctctgg
    18421 aaatctcccg cagcatacca gccgaacgac g
//
LOCUS       DBNGSX010000364         3830 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_308512, whole genome shotgun sequence.
ACCESSION   DBNGSX010000364 DBNGSX010000000
VERSION     DBNGSX010000364.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_308512"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="AC16CB_10960"
     CDS             complement(<1..949)
                     /locus_tag="AC16CB_10960"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HIR3343032.1"
                     /translation="MILGLILVPGVSRLSLRLGQVASPRQDRWHRKPTPILGGVAIYL
                     ATVGGLVVYAAINGEWGNIRWELLVVSSLVFLLGLYDDVKQMTPPAKLLGQILAAAAV
                     VFLGYTTTFFTPKITNNVVAQIPNIALTFVWIVGLTNAINLIDNMDGLAGGISLITIG
                     FLSYFFWIGGDFELLPIAMALAGGILGFLIFNFPPASIFMGDSGSLFLGFILAVLAIA
                     RQPQASNVFAVMGVPTLLFLLPILDTTLVTITRILRGQSPAQGGRDHTSHRLIAFGLS
                     ERQTLFVLFSIALISGIVAIGVETLDYWLSLAIVPLLVLS"
     gene            complement(979..>3830)
                     /locus_tag="AC16CB_10965"
     CDS             complement(979..>3830)
                     /locus_tag="AC16CB_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343033.1"
                     /translation="FVAILGNLRRQAQIDRIITTAVLTSLPVALYGILQRYDIDPIPW
                     AGDTSVRIAANMGNSIFVAAYLIMVFPLSVVRIVQSFNAILKDDERILAQVAQATIYV
                     FVGAVQVIGLYMSGSRGPALGWLAGSFFLFLLLALYWRKRWLTLVTIGAALAMGAFLV
                     VFNLEGGPLQNLRNSPAIGRFGLLLDPESNSALVRKYIWQGASELVRPHEPLEYPDGS
                     NDSFNALRPLIGYGPESMYVAYNPFYVPELAIVERRNASPDRSHNETWDALVITGILG
                     ILVYLLLFTMVFYYGMKWAGLIPGRKQRNLFLVVWLGVGFLGAAGMALWRGVEYIGVG
                     LPFGMLIGLLIYLTTVAISGMYNPPSTPAESARFLTLIVLIAAIVSHFIEINFGIAIV
                     STRTYFWTYAALLVVVGYIFPRIGQEGRIAENLFQNVDNAQDLTRRRSSSAKKKRRAR
                     SGPSKLGSNWFTSNRQSLISGLIIAIILITLGYDFIANPLGLDSASQILLSALTRLPN
                     MDYAFSLGMLMMLTTSWLFMGVVMKAESPTREDSDWWRDLAIILGVSLMVGLIYWLWH
                     ASGLATIAKNSASTFEDVLTQVGRFEDLLVRFNIFLFLLILALAAVISDEIPVRSKTT
                     KLGGILTAPIAMLVVISLSFITNTRIIQADIAFKIAEPFNRGGQWPAAIAIYNRANEL
                     APNEDYYYLFLGRAFLEHAKTLQDISERDRFIQEAENELLRAQKLNPLNTDHTANLAR
                     LYNMWASSSTSPEERERKGNISSEYFSKAVKLSRNSAILWDEWASLFLFVLQKPEEAF
                     PRLQRALEIDPKYHRTYAMLGEYYLRKAQAENDPVEVQKFLEEGSVNLKKALELPTPG
                     DSAAKWNYAQMLGGAQLQLGQVPAAIDSYLLALEFAPSGVDVWRLYEEIARLYARIGN
                     MANAQLYIQYALNDAPEDQKEQLRSLSAQLQNLTP"
BASE COUNT         1117 a          969 c          859 g          885 t
ORIGIN      
        1 gagagagaac cagcagtggt acgattgcca agctgagcca atagtcaagc gtctcaacgc
       61 caatagcaac aatcccagaa atcagggcta tagaaaaaag tacaaataag gtctgtcgct
      121 cggagagccc aaaagctatc aacctgtgag acgtgtgatc acgcccgcct tgcgctggag
      181 attgtccccg cagaatgcgg gttatggtta ccaaggtggt atccaatata gggagtaaga
      241 atagcaaagt aggcacaccc atcacagcaa atacattcga agcctgtggt tggcgtgcaa
      301 tcgccaggac agccaatata aaacccaaaa aaaggcttcc actatcgccc atgaagattg
      361 atgcaggagg aaaattgaag atcagaaaac ccagaattcc acctgcgagc gccattgcaa
      421 tcgggagcag ctcaaaatcc cctcctatcc agaagaaata gctcaaaaaa ccgatggtga
      481 tcaatgatat accccctgcg agaccatcca tattgtcgat caggttgatt gcattggtca
      541 aacctacaat ccagacaaaa gtcaacgcga tattgggtat ctgcgcaact acattgttcg
      601 tgatttttgg agtgaagaag gtcgttgtat accctagaaa tactaccgcc gctgctgcca
      661 gtatctgacc taacagcttg gcgggaggtg tcatctgctt cacatcgtca tataagccaa
      721 gcaggaaaac caggctagag acgaccaata gctcccatcg gatgttcccc cattcgccgt
      781 tgattgccgc gtatacaact aatcccccaa cggtagccaa atagatagcc actccaccca
      841 atattggtgt gggcttacgg tgccagcgat cttgacgcgg gctggctacc tggcctaatc
      901 gaaggctgag tctactgacg cctgggacaa gaattaggcc gaggatcaat gagatcccga
      961 aactaaccca tgaagtgatc aaggcgttaa attttgaagc tgcgcgctga gggaacgcag
     1021 ctgctccttc tgatcttcag gtgcgtcatt aagagcatat tgtatgtata gttgtgcatt
     1081 tgccatattc ccaatccgag cgtataggcg agcaatttcc tcatacaatc tccagacatc
     1141 aacgccagag ggggcgaatt ccagcgcaag caagtacgaa tcgatagctg ctggtacttg
     1201 tcccaattgg agttgtgctc cacctaacat ctgcgcatag ttccactttg cggctgagtc
     1261 gccaggtgtc ggaagttcca gggctttttt gaggttgacc gatccctctt ccaagaattt
     1321 ctgtacttcc acggggtcat tttctgcttg tgccttgcgt aaataatact cacccaacat
     1381 tgcatatgtg cggtgatatt tggggtcgat ctccagggct ctttgaagcc gcggaaatgc
     1441 ctcttctggt ttttggagta caaatagaaa gagcgaagcc cattcatccc ataaaattgc
     1501 gctgttgcga ctcaatttaa ccgccttgga aaaatactct gacgaaatat taccttttcg
     1561 ctcacgctcc tctggacttg ttgaggaaga tgcccacata ttgtacaaac gtgccaggtt
     1621 tgctgtgtgg tcggtgttca atgggttgag tttctgggct ctcaacaact cgttttccgc
     1681 ctcctggatg aatcgatccc gctcgctgat atcttgcaac gtttttgcgt gctcgaggaa
     1741 tgcgcgtcca aggaagagat aataataatc ttcattgggg gctaactcat ttgcccgatt
     1801 ataaatagcg attgctgcag gccactgtcc accacgattg aatggttcag ctattttgaa
     1861 agcgatgtcg gcctgaataa tgcgggtatt tgtaatgaaa gaaagactaa tgacaaccaa
     1921 catggcgatt ggcgccgtga ggattccacc gagtttagtc gtcttagacc gaacaggtat
     1981 ctcgtctgaa ataaccgctg caagcgcaag aattagtaag aataagaata tattgaagcg
     2041 aaccaacaaa tcttcaaatc gacccacctg tgtgagcaca tcttcgaaag tgctcgccga
     2101 atttttagca atggtcgcca atcccgaggc gtgccataac caatagatta atccaaccat
     2161 tagtgacaca cctagtatta tcgcaagatc tcgccaccaa tcgctatcct ctctcgtcgg
     2221 agattctgcc ttcatgacca cccccatgaa taaccaggag gtcgtaagca tcatcagcat
     2281 acccaacgaa aaagcataat ccatattcgg taagcgcgtc agcgccgaca gcaagatttg
     2341 tgatgcagaa tccaagccga gtggattagc gataaagtca taacctaaag taatcaatat
     2401 aatagcgatg atcaatccag aaatcaacga ctggcggtta gatgtgaacc aattcgaccc
     2461 taactttgat ggtccgctgc gagcgcgacg ctttttcttc gcagaagaag accttcgcct
     2521 tgtcaaatcc tgagcgttat ccacattctg gaataagttc tccgcaattc tcccttcttg
     2581 accaattcgc ggaaagatat atcccacaac tactaataat gcggcatagg tccaaaaata
     2641 ggtgcgtgtt gagacaatcg caatcccaaa gtttatctca ataaaatgcg aaacaatcgc
     2701 tgcgataagc acgattaatg tcaagaatcg ggctgattca gcaggtgttg agggaggatt
     2761 gtacatgcca gaaattgcca ccgtggtcaa gtagattaat agaccgatca acatcccaaa
     2821 gggcaaaccc acaccaatat actccacgcc tctccacaaa gccatgcctg ctgcccccag
     2881 aaaacccacc ccaagccaga ctaccagaaa cagattacgt tgcttccgac ccgggattaa
     2941 tccagcccat ttcataccgt aataaaacac catcgtaaac aataacaggt aaaccaaaat
     3001 gcccaaaata ccagtaataa ccagcgcatc ccatgtctcg ttgtgagatc tatccgggga
     3061 ggcgttgcgt cgctcaacaa tagccagctc gggtacatag aatgggttat atgccacata
     3121 catgctttcc ggtccatatc caatcaatgg acggagtgca ttgaaggaat cattgcttcc
     3181 atcgggatat tctagaggtt cgtgtggtct aaccaattcc gaagcaccct gccagatata
     3241 tttccgcact aaggcgctgt tactctcggg atccagtaac aacccaaagc ggccaatagc
     3301 cggtgaattt cgcagatttt gtaaaggacc accctctagg ttaaaaacaa caagaaaggc
     3361 gcccattgcc agcgctgcgc caattgttac gagggtaagc catctcttac gccagtatag
     3421 ggcgagcagc aagaaaagga agaacgaacc agctaaccaa cccaaagccg gtccgcgact
     3481 accactcata tataatccaa tcacctggac tgcaccaaca aacacataga tcgtcgcttg
     3541 tgctacctga gccagaatgc gctcatcatc ttttagaata gcgttaaacg actggacgat
     3601 acgcacgact gatagtggaa agaccatgat caggtaggca gccacaaaga tcgagttgcc
     3661 catgttggcg gcaatccgca cagaagtatc gccagcccat gggatgggat cgatatcata
     3721 gcgttgcaga atcccatata acgcaaccgg caggctggtc aagaccgctg tcgtaataat
     3781 tcgatcaatc tgggcttgcc ggcgaaggtt ccccaagata gcaacaaata
//
LOCUS       DBNGSX010000365         2131 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1071253, whole genome shotgun sequence.
ACCESSION   DBNGSX010000365 DBNGSX010000000
VERSION     DBNGSX010000365.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1071253"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..627)
                     /locus_tag="AC16CB_10970"
     CDS             complement(52..627)
                     /locus_tag="AC16CB_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HIR3343034.1"
                     /translation="MDTPSTSEELLNFFKALADANRLKIVGLLAQQPTTVEGLAAALG
                     LGASTVSHHLSKLAKAGLVSARTDGHYYIYSLETDALQAMAERLLSADELPRLSDDAD
                     LDAYDRKVLANFLDAEGRIKAFPAQEKKFQVILQHVVQAFEPGQHYTEKQVNEILGRY
                     HKDTASLRRGLIDYRLMARQSGGGEYWRTEA"
     gene            830..1099
                     /locus_tag="AC16CB_10975"
     CDS             830..1099
                     /locus_tag="AC16CB_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HIR3343035.1"
                     /translation="MPEICRFFGIIIRMFAEPGAPHHRTHFHAYYQNHVAIFSIDKVE
                     LINGSLPSRERRLVEAWAELHQGELSENWERLQSGQLPYKIAPLR"
     gene            1121..1426
                     /locus_tag="AC16CB_10980"
     CDS             1121..1426
                     /locus_tag="AC16CB_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HIR3343036.1"
                     /translation="MKHPIYRVVEFEIVNSYTLRVIFDDGKEQVIDFRPVLIGELYGP
                     LRDKTLFKKVEIDPEVHTLVWPNGADFDPATLHDWPENVDSMQELAKRWAAKKVEAT"
     gene            1566..1991
                     /locus_tag="AC16CB_10985"
     CDS             1566..1991
                     /locus_tag="AC16CB_10985"
                     /inference="COORDINATES: protein motif:HMM:NF048429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7670 domain-containing protein"
                     /protein_id="HIR3343037.1"
                     /translation="MTSIFVNILAIAIVGFVLIRKGQEQRVWDQPTASRLMIVGLIPL
                     VLQIAIFLLFGLGEMASGELSGAGHLLPAVATLVLVLLSWKRPIEGGAALFVIGAVTT
                     TDVSEATALLIMAAPQIFSGLMFLLAGWIAQGGASPEGN"
BASE COUNT          488 a          557 c          567 g          519 t
ORIGIN      
        1 cttagtgtta aatcccttcc aatgttttgt catttcatcc gtcaggcgtg ctcaagcctc
       61 ggtgcgccag tactcccctc cgccgctttg gcgcgccatc aggcggtaat cgatcaggcc
      121 gcggcgcagg gatgcggtgt ctttatggta gcgccctagg atctcgttca cctgtttctc
      181 agtgtagtgc tgcccgggct cgaaggcctg taccacgtgc tgcaggatga cctggaattt
      241 cttctcctgg gcgggaaaag ccttgatgcg cccctcggca tccaggaaat tcgccagcac
      301 tttgcggtcg taagcgtcta ggtcggcgtc gtccgatagg cggggcagct catcagcact
      361 cagcaaacgc tcagccatgg cttgcagagc gtcggtctct agcgagtaga tgtagtagtg
      421 cccgtccgtg cgcgcagaca ccagcccagc cttagccagc ttggacaggt ggtgcgagac
      481 agtggaagcg cccaggccga gcgccgcagc cagcccttcg acggtggtgg gctgctgcgc
      541 cagcaatccg acgatcttca gccggttggc gtctgccagg gctttaaaga aattgagcag
      601 ctcttcgctg gttgatgggg tgtccacaat ccgaactcct tcgtaacatt tcgactatct
      661 tcgaaatgat tttatccatt tcgttgggct tgtcaaggat gaacatgcga tttcagtcat
      721 taataacgaa gagcgtacgc cctgaaaata taggtctgac gttggcggcc gttctaggca
      781 actttgatgt aaaataatat aggatactac gatatggaag gaaacatacg tgcccgagat
      841 ctgccgcttt tttggcatca ttattcgcat gtttgctgag ccaggtgctc cccatcaccg
      901 tactcatttt catgcttatt accaaaacca tgtggccatc ttcagtatag ataaagtcga
      961 gctaatcaat ggcagcttac catctcggga gagacgattg gtggaggcct gggctgaatt
     1021 gcatcaaggg gagctctcag aaaactggga gcgtttacaa tccggtcagt tgccttataa
     1081 aattgcaccg ttacgatagg agaattggag aaaagagata atgaagcatc cgatttatcg
     1141 tgtggtagag tttgaaatag tcaactcata caccctgcga gtgatattcg acgatggaaa
     1201 ggaacaggtc attgattttc gcccggtcct tattggtgaa ttgtatgggc cattacgaga
     1261 taaaacacta ttcaaaaaag tagagattga tccagaagtt cacacattgg tctggcctaa
     1321 cggtgccgat tttgatcctg ctacactgca cgattggcct gaaaatgtgg acagtatgca
     1381 ggagttagca aaacggtggg cagcaaagaa agttgaagcc acataggaaa gcgtttccca
     1441 aaaaacatct cccagatatc atacaagtaa aaaatcagtc aacccaccgc ctgtcctggc
     1501 gggcagtcca gggtggctgc cagcatctcc agtttgatgg ggaatagtgc acaaaggagg
     1561 atcgtatgac ttccatcttt gtcaacatcc ttgccatcgc cattgtggga tttgtcctta
     1621 tccgtaaggg acaggagcag cgagtttggg accagccaac agcaagccgg ctcatgatcg
     1681 tcggtctcat tccgctggta ctgcagattg ccatcttcct gttgtttggt ctaggcgaga
     1741 tggcgtccgg cgagctcagc ggggccgggc accttctgcc tgccgttgca accctggtgc
     1801 tagtgctgct ttcctggaag cggcctatcg aaggcggcgc agccctgttt gtgatcggcg
     1861 cagtgacgac tacagatgtc tctgaggcta cagcgttact gatcatggca gccccacaaa
     1921 ttttctccgg cctgatgttc cttctcgccg gatggatcgc acagggagga gcgtcacccg
     1981 aaggcaatta acctgccaaa cttcccctga ccttcttcct cagctcatcg atgaccagca
     2041 attcccccgt ctcggtctca taataccagt tgtcccagcc attgcatggg ctgccgccca
     2101 tcaggtggct acccacagcg tggatcgagc c
//
LOCUS       DBNGSX010000366         9207 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_933303, whole genome shotgun sequence.
ACCESSION   DBNGSX010000366 DBNGSX010000000
VERSION     DBNGSX010000366.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9207)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9207)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9207
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_933303"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="AC16CB_10990"
     CDS             <1..960
                     /locus_tag="AC16CB_10990"
                     /inference="COORDINATES: protein motif:HMM:NF013844.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HIR3343038.1"
                     /translation="SVEFDLETLRPTYHLTVGLPGRSNALAIAQRLGMPDVIIDTARS
                     ELSPSDLRAEDLLDEIHRQRDLSRQARLTAENARNQAEKLRLELAERLEKIEDERLQV
                     LEKARLEAEAQIQALQDELRELRRQLARARQPLEALRSVEEQIEDLEQVVDKQIERRI
                     PEVGETPIYRPVRLGDKVRLRTLNTQGVVTSLSEEEAEIQVGRLRVRTRIADLQPLDD
                     EAQPLSEAVSMGDHPSAELTTSPGFELDLRGQRADEALEILERYLDSAYLAGLPFVRI
                     IHGKGTGKLRQAVRQALQSHPNVRSFEAGGDKEGGEGVTVAKL"
     gene            1008..2210
                     /locus_tag="AC16CB_10995"
     CDS             1008..2210
                     /locus_tag="AC16CB_10995"
                     /inference="COORDINATES: protein motif:HMM:NF020246.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HIR3343039.1"
                     /translation="MQNLSRRFVTLILILALVQVSCNLLQRLPPPAATPTLEVSPTSP
                     ATPTLVPSATATPTPTITPTPLPTSTPTQPPLADLGTPLPAFLEPISINNVTRVNPLA
                     ELRKEAITKLAWSLDSTTLAAGGNSEVDFFDIRTLTKQRTLKVDEGLVSFGLSPDGRY
                     LASGNNTGSEQSGYTGSISFWRLSDEERLFIFYLDRRGVSDLSFSPNGRTFAAAVTSK
                     EYIDNGFVFWNTQTFEITRTLRTGGALDMAFSPDGRYIASTPDRFAIRLWEMRYGQYL
                     YELPTSFTGAVNTLAFSPDGSILATGHYDGVIRLWDVAKGELLQEFSAIGVVESLVFS
                     PDGTLLASGEGYTSTAIRLWDLASGQNLRTLEGHTHAVDNLVFSPDGRLLASGSYDGT
                     LRLWGVTP"
     gene            2216..3334
                     /locus_tag="AC16CB_11000"
     CDS             2216..3334
                     /locus_tag="AC16CB_11000"
                     /inference="COORDINATES: protein motif:HMM:NF024298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HIR3343040.1"
                     /translation="MPVLAALGLAACASLPTTPTPTHPPIQTKQPFQVAETSAPFATD
                     TAVASPSVTLSPLPPSLTPTPPTISPANASSLIPRAKIQFSSWELVLAAAWSPDGETL
                     AVAAGEKIHLYRAGSHEERLTLNPGVWTPGLAFSPDSRTLACGGRDGVVQLWDVASGD
                     IRFSMPAHKKGVNSVAFSPDGRMLASAGNDGMVRLWDSENGGPQGQMIGGAYAIPSVV
                     FTQDSASLAIANGDIIRFRDVETQRFVLTLRGDSSFYSLALAPDGQFLASGDSNNTVL
                     LWDLSTEKVSHSLQAHNGDVSRAAALIWRVAFSPDARLLVSAGGDQTVRVWDATTGQL
                     LSTLIGHKAAVTSLAFSPDGRWLVTGGLDATVFVWGSK"
     gene            3467..4360
                     /locus_tag="AC16CB_11005"
     CDS             3467..4360
                     /locus_tag="AC16CB_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3343041.1"
                     /translation="MRLLCLSLLLILSILLSACVASPTSPSDVEASTYIQNKGSDTIV
                     NLALAWAERYQQEHPNVRISVTGGGTGTGMAALISGTVDIANASRQIKPEEVDEANKN
                     GVEPQEYVIARDAIAVIIHPDNPINKLTLEQVSRIYRGEINNWKELGSYDRPIVRVSR
                     ETNSGTHVYFLESVIRLGNDDNKYFFSTDTLLLPSSEGIIAEVSENPNAIGYDGLGYV
                     TPSVKVVALSEDPAGHYVLPSSETVNNGSYPVSRDLYMYTGSAPSAAAQAYLQWIMEA
                     EAQEIVIELGFVPVNGQAEIR"
     gene            4367..5263
                     /gene="pstC"
                     /locus_tag="AC16CB_11010"
     CDS             4367..5263
                     /gene="pstC"
                     /locus_tag="AC16CB_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIR3343042.1"
                     /translation="MKSDATKNEFWITLLIRISGYSSILFVILILYFLLREGLPTLGD
                     VSFFSLFGTRWYPIEDYFGVLPLLSGSLIVTVGAMLVALPFGVGTAVFLSEVSPHWLK
                     SVLKPVIEVMAGLPSVVLGFLGIQIAAPLFRRFLDLPTGLTGFTGAILLGFISIPTIV
                     SIAEDALNSVPATYRHGALALGATRWQTIWGVTVPAARSGILTAVMLGVGRAIGETMA
                     VMMVTGNAPVLPFRLDALFLPVRTMTATIASEMGEVAAGSSHYHVLFFIGIILFGVSL
                     IVNIIASSIALRSRSRAERLLS"
     gene            5275..6141
                     /gene="pstA"
                     /locus_tag="AC16CB_11015"
     CDS             5275..6141
                     /gene="pstA"
                     /locus_tag="AC16CB_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR3343043.1"
                     /translation="MDRSDHITNVRRIEWIGINLLRLITLITALPVIGVILYILARGM
                     PAMSLEFLTQMPREGMRAGGILPTIIGTFYLTLGTAIFSVPLGVAAGIYIAEYAPDNY
                     LTRLIRIAIINLAGIPSVVYGLFGLGLFVLFMGFGTSILAASLTLSIMTLPVIISTTE
                     EALRSVPNSFRVVSLSMGGTKWQTVWRITLPQALPGVITGIILGLERAAGETAPILFT
                     GAAFFLPRLPHSPLDATMALPYHIYVISTSVPGMPVQIQYGTVLVLLVFVLGMNLIAT
                     LIRNRARARRQW"
     gene            6149..6907
                     /locus_tag="AC16CB_11020"
     CDS             6149..6907
                     /locus_tag="AC16CB_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HIR3343044.1"
                     /translation="MEKLIIVEKLNLVYSDGVESLVDVNLDIIANSVNVLFGPAGGGK
                     SSLLRAFNRLNDLADVASISGRILFHGMDILDPKTDVIDLRRKIGMVFSRPVPLPLTV
                     YENVSYGLQLAGERSKSRLDEAVEEALRLAVLWDEVYDRLNDPAYSLSGGQQQRLCLA
                     RVLALKPDVVMLDEPTSALDPVSTARIEALLLDLRERFTIILVPHNVQQAARTADYAG
                     FFLQGKLVDYAPGKELFLNPKLKETQDYVTGRFG"
     gene            7333..7962
                     /locus_tag="AC16CB_11025"
     CDS             7333..7962
                     /locus_tag="AC16CB_11025"
                     /inference="COORDINATES: protein motif:HMM:NF025338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3343045.1"
                     /translation="MTTQTRQLIINTAIDLFNLQGTSNVSTNHIAAAMDISPGNLYYH
                     FRNKEEIILAIFEQMVAEMDQVWKFQPAFSLKNFVELAFQTIEGLARYHFFQRETIPL
                     MQRDTVLKARYKAMQQYRLAQLEALLQHFVTSGLLVQFPNPQVMASLAKACWMIGDRW
                     LVMLDMEGKAPTRKNIAEGILLILQLLEPYFSQQGKEEYSRLIQNLEEI"
     gene            7959..8678
                     /locus_tag="AC16CB_11030"
     CDS             7959..8678
                     /locus_tag="AC16CB_11030"
                     /inference="COORDINATES: protein motif:HMM:NF025687.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HIR3343046.1"
                     /translation="MKDSRIIKVFSVSSLILTILFVAVFTYLAINYDYPDNLDYPAQT
                     TLPSIDQAGSDWRAIWFFYALLPVGYILVGMTAYLVLKPIDKGLIRMGVLFTVLASLF
                     GTLGLARWPTIMPPIAKAYQTADASQRAVLEAIFNGVNLYLGNYLGEFMSEFLFGAFI
                     VLVGITIFAAKGNGYPRWFGVISIISGVLIVMGSTRMFFELTKAANEYTTMVLPVYLI
                     LFSGLSYRAASMLRTISKTAG"
     gene            8780..>9207
                     /locus_tag="AC16CB_11035"
     CDS             8780..>9207
                     /locus_tag="AC16CB_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343047.1"
                     /translation="MILKKYLITSIITGLLIASCNAASRLAAPTSDQATIPPPEIIDT
                     ATPPAPTAIPSAAAITTNYQNHSLGISLDYPAVWHVQEEIPDQPFVVSLTSFDPASPP
                     HKLEWDSQTVSISLRLLPQENTPQDLNSWAESAKQEAVAD"
BASE COUNT         2133 a         2538 c         2314 g         2222 t
ORIGIN      
        1 agtgtagagt tcgatctaga gacactgcgg ccgacctacc acctgactgt cggccttcca
       61 ggccgatcga atgccctggc tattgcccag cgcctgggca tgcctgatgt tatcattgac
      121 actgcgcgct cggagctcag tccaagcgac ctgcgagccg aagatctcct ggatgaaatt
      181 catcgccaac gcgatctttc tcgtcaggct cgtctcaccg ctgaaaatgc ccgcaaccaa
      241 gccgagaaac ttcgcctcga actggcagag cgtctggaga agatcgaaga tgagcgcttg
      301 caggttttag agaaagcgcg cctggaagcc gaggctcaaa tccaagccct acaggatgag
      361 ctgcgcgagc ttcggcgcca gcttgcccgg gcgcgacaac cactggaagc cctgcgaagc
      421 gtagaagaac aaattgaaga tctggaacag gttgtagata aacaaatcga acgccgaatt
      481 cctgaagtag gtgaaacacc aatttaccgc cccgttcgac tgggtgataa agtccgactg
      541 cgaactctga atacccaagg cgtggttact tcattaagtg aagaggaggc cgagattcag
      601 gtgggcaggt tgcgcgtccg aacacgcata gctgacctgc aaccgttgga cgacgaggcc
      661 caaccgctat ccgaagcggt gtccatgggt gaccatcctt ctgccgaact gaccacatct
      721 cctggctttg agctagacct gcgcggacag cgcgccgatg aagccttgga gatcctagaa
      781 cgctacctgg actctgccta cctggccggc ctacccttcg ttcgcatcat ccatggtaag
      841 ggcactggca agctgcgcca agcggtacgc caagcgcttc agagtcaccc aaatgtgcgc
      901 tctttcgaag ccggaggaga caaagagggc ggagaagggg tcactgtggc aaagttgtag
      961 actgactgag agcagtgaga aaaatctttg ccagttggaa cgcgcatatg caaaatctat
     1021 cccggcgatt tgtcacctta atactcatcc tggcgctcgt ccaagtgagc tgcaatctac
     1081 tgcaacgctt accgcctccc gcggctaccc caaccttaga ggtatcgccc acatcaccgg
     1141 caacgcccac tttggtgccc tcagccaccg ccacgccgac tccaaccatc acccctaccc
     1201 ctttgcctac ctcaacgccc acacagccgc ctctcgccga cctaggaact ccattacccg
     1261 catttctaga gcccatcagc ataaacaacg tcacccgggt aaatccgctg gcagaattgc
     1321 gcaaggaagc tatcaccaaa ctagcctggt cactagacag cacaactcta gccgcaggcg
     1381 gcaattccga ggtggacttt ttcgacattc gcacgctgac aaaacagagg actttaaagg
     1441 tagatgaggg tttggtgagt ttcggcctaa gccctgatgg gcggtatctt gcctctggga
     1501 ataacacggg gtcagagcag tcaggctata ctgggagcat ctctttttgg cgcctatccg
     1561 acgaggagcg tttgtttatc ttttacttgg atcggcgagg ggttagcgat ttatccttct
     1621 cccccaatgg cagaaccttt gccgcagccg tgaccagtaa agaatatata gacaacggct
     1681 tcgtgttctg gaacacgcaa acctttgaga tcactcgcac attacgcacc ggcggtgcgc
     1741 tagacatggc cttctcgcca gatgggcgct atatcgcctc tacacccgat cgattcgcca
     1801 tccgcttatg ggaaatgcga tacgggcagt acctgtatga actgcccacc tcctttaccg
     1861 gagcggtcaa caccttggct ttttcgcctg acggaagtat cctggcaacc gggcactatg
     1921 atggcgtcat ccgcctgtgg gatgtagcca aaggtgagct acttcaggaa tttagcgcca
     1981 ttggggtagt ggaaagcctt gtcttcagtc ccgacggtac cctgctggct tctggggaag
     2041 gttacacaag cactgccatc cgcttgtggg acctggcgag tggtcagaat ttgcgtaccc
     2101 tggaagggca tacccatgcg gtggacaacc tggttttctc acccgatggt cgcttgctcg
     2161 cctcgggctc ttacgacggc accttacgcc tttggggagt gacgccttga gggttgtgcc
     2221 ggtgctggcc gcattaggcc ttgcagcttg cgccagcctg ccgacaacac ccacccccac
     2281 tcatccacca atccaaacca agcagccttt ccaagtggcc gagacatctg cgccatttgc
     2341 caccgatact gcagtggctt ccccatccgt taccctctcc cctctacccc catctctaac
     2401 gcccactccg cctacgatct ctcctgctaa cgcctcttcc ctcataccac gggcgaaaat
     2461 acagtttagc tcttgggaac tggtacttgc ggcggcctgg tctccagatg gcgagacgct
     2521 ggcggtagcc gctggggaaa agattcacct ttatcgagcg ggatcacacg aggagcggct
     2581 caccctgaat ccgggtgtgt ggacgcctgg actggcattc agcccagata gccgcacgct
     2641 agcttgtggt ggacgggatg gcgtagtaca actgtgggat gtcgcatcag gagatatccg
     2701 cttctctatg ccggctcaca agaaaggggt caactccgtc gcctttagcc cagatggcag
     2761 gatgctggca tcagccggca atgatggcat ggtacgccta tgggattctg agaatggcgg
     2821 tcctcaaggg cagatgatcg gtggagcgta cgctattcca tccgtggttt tcacccaaga
     2881 cagcgccagc ctggcaatcg ccaacgggga tattatccgt ttccgggatg tggaaacgca
     2941 gcgcttcgtc cttacgttgc gcggtgattc ctccttctat tccctggctc tcgcgcctga
     3001 tgggcagttc ttagcctcag gcgatagcaa caacaccgtg ctgctatggg atctttcgac
     3061 cgagaaagtc agccacagct tgcaggctca taatggggat gtttcacggg ctgctgcgct
     3121 gatctggcga gtcgctttta gccccgacgc tcgcctgcta gtctctgccg gaggggatca
     3181 gacggtcagg gtgtgggacg ctaccacggg tcagctgctg tcaaccctga ttggtcataa
     3241 ggctgcagtc accagcctgg catttagccc agacggccgc tggctggtta ccggcgggct
     3301 agatgcgacc gtattcgtct ggggatcgaa atagggaaaa tatttctctc tcacaacagc
     3361 tccaacatat gccttcatca atatgcgtag aatcccgtct caacctgcag agatgtcagc
     3421 gatctttaag gtttatccca aagagattag actataattt gttgaaatga ggcttttgtg
     3481 tctctctcta cttttaatct tgagcatcct gcttagtgcg tgtgtagcaa gccctacatc
     3541 cccttcggac gtagaagctt ctacctacat acaaaataaa ggatccgaca ctatcgtcaa
     3601 cctggctctg gcttgggctg agcgctacca gcaggagcat cccaatgtac gtatctcggt
     3661 aaccggtggt gggacaggca ctggtatggc agcgctgatc agcggcactg tggacatagc
     3721 caacgcctct cgccagatta aacccgaaga ggtcgatgaa gctaataaaa atggggtgga
     3781 gccgcaggaa tacgtgattg ctcgcgatgc aatcgcagtc attattcacc cagataaccc
     3841 cataaataaa cttaccctcg agcaggtctc aagaatatat cgcggggaaa tcaataactg
     3901 gaaagagctt ggctcgtatg accgcccgat tgtgcgcgtt tcgcgggaga ccaactcagg
     3961 tacccacgtt tactttttag agtcggtgat ccgcctgggt aatgatgata acaaatattt
     4021 cttctccacc gacacgctcc tgctgccatc atcggaaggc atcatcgcag aggtcagtga
     4081 aaacccaaac gccatcggat atgatgggct aggctatgta acccccagtg taaaagtggt
     4141 cgctctatca gaagacccgg ctggtcacta cgtgctacca tcgtctgaaa cagtcaacaa
     4201 cggaagttac ccggtatcac gtgacttgta catgtacacg gggagcgccc catccgcggc
     4261 agcgcaggct tacctccaat ggatcatgga ggcggaggcg caggaaattg tcattgaatt
     4321 aggatttgta cccgtaaatg gccaagcaga aatcaggtaa acatcaatga aatcagatgc
     4381 aacaaagaac gaattctgga tcacactgct tatacgcatc tcaggatact caagcatatt
     4441 atttgtcatc ctgattctat atttccttct tcgcgagggg ctgccaacgc tgggagatgt
     4501 ctcatttttc agcctgttcg gcactcgttg gtatccgatt gaggattact ttggagtgct
     4561 tcctctcctc agcggttcac tcatcgtcac cgttggcgcg atgcttgtcg ccttgccgtt
     4621 tggcgtgggc actgctgttt tcctttcgga ggtatctccc cactggctca agtcagtgtt
     4681 gaaaccggtc atcgaagtca tggccggtct accttcggtg gtgctgggct ttctaggaat
     4741 ccagatcgcg gctcccctct tccgccgttt cctggattta cccaccggat tgaccggatt
     4801 cacgggtgcg atcctgcttg gctttatttc catcccgaca atcgtctcca tcgccgaaga
     4861 tgcgctgaat tcagtgcccg ctacataccg gcatggggcg ctggcactgg gtgctacccg
     4921 ctggcagacg atctgggggg ttacggtgcc cgcggcacgc tcaggcattc tcaccgcagt
     4981 gatgctggga gtcggccggg caatcggcga gaccatggcg gtgatgatgg ttaccggaaa
     5041 cgccccggtg ctgccatttc gcctggacgc attgttcctt ccagtgcgca ccatgaccgc
     5101 caccatcgct tcggaaatgg gagaagtagc tgcaggctcg tcccactatc atgtactctt
     5161 ttttatcggc attattctgt ttggcgtctc gctaatcgta aacatcatcg catcttcgat
     5221 tgcattacgc agccgctcgc gagccgaaag gcttctttct taggtagaga gctgatggat
     5281 cgatctgacc acattacgaa tgttcgccgc atcgaatgga ttggcattaa cctgctgcgc
     5341 ttgatcaccc tgatcaccgc tctgccagtc atcggtgtta tcctatatat ccttgcacgc
     5401 gggatgcccg caatgagcct tgagtttctc acccagatgc cgcgtgaagg catgcgcgcg
     5461 ggtgggatat tgccgaccat tatcggcact ttctacctga cccttggcac agccatattt
     5521 tctgtaccct tgggtgtggc cgccggcatc tatatcgccg aatacgcacc agataactac
     5581 ctaacccgtc tcatccgcat cgccatcatt aacctggctg ggataccttc tgtagtctac
     5641 ggcctctttg gcctgggctt gtttgtcctt ttcatgggtt tcggcaccag tattctggct
     5701 gcctcgctca ccctctcgat tatgaccttg ccggtgatta tcagcaccac cgaagaggcg
     5761 cttcggtctg tccccaactc gtttcgcgtg gttagcttgt ctatgggggg gaccaagtgg
     5821 caaaccgttt ggcgtatcac cctgcctcag gcacttccag gcgttattac cggtatcatc
     5881 ctcggcctag agcgtgcagc gggggagaca gcacccatcc tcttcacggg cgccgccttc
     5941 ttcctgcccc gcctgccaca ttcaccactg gacgccacca tggcactccc ttaccacatt
     6001 tacgtgattt cgacctcagt gccgggtatg ccggttcaga tccaatacgg aactgttctg
     6061 gtgctgctgg tatttgtact gggcatgaac ctgatcgcaa cacttatccg caaccgtgcc
     6121 agggcgcggc gccaatggta ggtccaacat ggaaaaactg atcattgtcg agaagttgaa
     6181 cctggtctat tcagatggcg tcgagagcct ggtcgatgta aacctggaca tcatcgctaa
     6241 ttcggtgaat gtgctttttg gccctgcagg tgggggcaaa tcttccctgc tgcgcgcctt
     6301 caaccggctg aatgatctgg cagatgtagc cagcatttcc ggacgtatcc ttttccatgg
     6361 gatggacatc ctcgacccga aaactgatgt gatcgatttg cgccgcaaga tcggcatggt
     6421 cttttcacga cccgtaccgt tgccgttgac agtttacgag aatgtgtcct atgggctgca
     6481 actagctgga gaacgtagta agagccgtct ggacgaagca gtcgaggaag cactgcgcct
     6541 ggcagtactg tgggacgagg tgtacgacag gctcaacgac ccggcttact cgctatctgg
     6601 gggacagcag cagcgacttt gcctggcgcg agtgctggcg ttaaaacctg atgtagttat
     6661 gctcgatgag cccacctcag cactggatcc cgtatccacc gcacgcatcg aggctctact
     6721 gctagacctg cgtgaacggt tcaccattat cctggtgccg cacaatgtgc agcaggcagc
     6781 caggaccgca gattacgccg gcttcttcct gcagggcaag ctggtagatt atgcccctgg
     6841 caaagaatta ttcctgaatc ccaagctcaa agagacccaa gattacgtca ctggaagatt
     6901 tggttaaatc gtagatcgct ggtgttgaaa cgtcagcgat gcagatcata tactgcacaa
     6961 cgacatccag gggggtgaat tgaattttat ctagccaaat tgattgaata tccccattca
     7021 attacgcggt taaattcctt tggatatgct ctgacccctt attatccgct tggtcttcaa
     7081 gtaagcttcc acgaagcagg ttttattctc gagcttggtt aaaccgaaca aagctgaatc
     7141 tgccgattgt ctaaatccag ttactcatcc ttcgcgatga agaccctcaa atcccaagaa
     7201 ccaggtagcg aattattgag atgcgagaat atttacaaac gcatttcaag gctactgggc
     7261 gtgacaaaac ccttgacaaa ttagagtatt tactctataa tagagtaaat actctaattg
     7321 ttaggaggct tgatgacaac acaaaccaga caactgatca tcaatacagc tatcgacctg
     7381 ttcaatctgc aaggcacctc caacgtctct acgaaccaca tcgctgcggc aatggatatc
     7441 agtccgggta atctctatta tcactttcgc aacaaggaag agatcattct ggcaatcttt
     7501 gagcagatgg tggctgagat ggatcaggtt tggaaatttc agccagcatt ctcgttgaaa
     7561 aacttcgtgg aactcgcatt tcagactatt gagggattag ctcgctatca cttcttccag
     7621 cgtgaaacta ttcctctgat gcaacgcgac accgttctaa aggcacgtta caaagcaatg
     7681 cagcaataca ggctggctca gttagaagct ctcctgcaac attttgtcac ctcaggtctg
     7741 ctagtacaat ttccaaaccc acaggtgatg gcctcactgg cgaaagcctg ctggatgatt
     7801 ggtgaccgat ggctggtcat gctggacatg gaaggcaaag ctcctacccg caaaaatatt
     7861 gctgaaggaa ttctcctgat tctgcaactg ctagaaccct acttttccca acaagggaaa
     7921 gaagaatact cacgcttgat ccaaaatttg gaggagatat gaaagacagt cgcatcatca
     7981 aagtattttc ggtatccagt ctgatactga cgattttgtt cgtggcagtg ttcacttacc
     8041 tggcgatcaa ttatgattat ccggataatc tggattaccc tgcccaaact accctgccga
     8101 gcattgacca agcaggttcc gattggcgcg caatctggtt cttctacgcc ctgctgccag
     8161 ttggttatat cttggttggg atgaccgcat atctggtcct caagccaatc gacaagggtc
     8221 tcattcgtat gggcgtgctt ttcactgtcc tggcgtcttt atttgggaca ctcggtctgg
     8281 cgagatggcc tacgattatg ccgccgattg caaaagccta ccagacagcg gacgccagcc
     8341 aaagagcagt actggaagca atcttcaacg gagttaacct ctacctggga aattacttag
     8401 gcgagtttat gagtgaattc ttgtttggcg ctttcatcgt cctggtaggc attacaatat
     8461 ttgctgcaaa gggaaacggt tatcctcgat ggtttggtgt aatttcgata atttctggtg
     8521 tcttaattgt gatgggctct acacgcatgt ttttcgagct gacaaaagct gcaaatgaat
     8581 atacaacgat ggtattacca gtatacttaa ttttatttag cggactgagt tatcgagcgg
     8641 cctcgatgct acgcactatt agcaaaacgg ctggctaggc gaaactatcg aaggaacaat
     8701 ttgaaccttt taaccgtcca taaggttgtt attaagctat aaaaatcgta tctctgctct
     8761 gcttggattt ggtgaacaaa tgattctcaa gaaatatctt atcacaagca taattactgg
     8821 tttgctcata gcttcctgta atgcggcatc tcgattggca gcaccaactt ccgaccaggc
     8881 aacgataccg cctccagaga ttattgatac tgctacaccc ccagcaccaa cagctatccc
     8941 ttcagctgca gcgatcacta ccaattacca aaaccattct ctaggcatca gcctggatta
     9001 tcctgcggtc tggcacgtgc aggaagaaat tccagatcag ccattcgtcg tctctctgac
     9061 ctccttcgac cctgcctctc ccccgcacaa gctggagtgg gatagccaga ctgtgagcat
     9121 cagcctccgc ctgcttccgc aggagaatac acctcaagac ctgaatagct gggcagagag
     9181 cgccaaacag gaagcagttg cagatca
//
LOCUS       DBNGSX010000367         1994 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_517472, whole genome shotgun sequence.
ACCESSION   DBNGSX010000367 DBNGSX010000000
VERSION     DBNGSX010000367.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_517472"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="AC16CB_11040"
     CDS             complement(<1..348)
                     /locus_tag="AC16CB_11040"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR3343048.1"
                     /translation="MDVITKTFITIGTILLFAPAIDEIARRTKLPRVTLLLLLGILIG
                     RFAQDLLPAFGKTWFQVVTDLALLMIGFLLGEKLIFSFLRKNARSVLWISIGEVLITV
                     VIMFAVLFLIGVPI"
     gene            complement(393..1513)
                     /locus_tag="AC16CB_11045"
     CDS             complement(join(393..1249,1249..1513))
                     /locus_tag="AC16CB_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611756.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HIR3343049.1"
                     /translation="MGRKRYQPEQIIGYLREAEILHSKGSTIPDICRQIGITEQTYYR
                     WRKEYGSLSVDQAKRLKEMEKENARLRKLVADLSLDKAILKEVAGGKVLSPTKKRRAV
                     FHVMEQFDVSERRVCRALNQPRSTQRYRPRTRDDEDQLTKRMIKLATKYGRYGYRRIT
                     ALLRREGFKVNHKRVERLWRREGLKVPQKQPKRKRLWLNDGSCIRLRPQFKDHVWSYD
                     FVTTRTEDGLPLRILTLIDEYTRECLALKVARRLRSQDVLEQLGYLFIYRGLPGFIRS
                     DNGPEFTAKAVRYWLERLGVQTLFIEPGSPWENGYNESFNGKLRDELLNGEIFTSLLE
                     AQVLIERWRKEYNQFRPHSALGYRPPAPETIKQNMEILT"
BASE COUNT          554 a          498 c          398 g          544 t
ORIGIN      
        1 aatgggaaca ccgatgagga aaagaaccgc aaacattatt acaaccgtta tcagcacctc
       61 gccgatcgag atccataata cagaccgagc attttttcta aggaaagaga atattaattt
      121 ttcacccagc aagaatccaa tcatgagaag ggctaaatct gtaaccactt gaaaccaagt
      181 cttaccaaac gcgggtaaaa ggtcctgggc aaaacgacca atcaatatac ccaataatag
      241 tagcaaagta accctaggaa gctttgtgcg gcgggcgatc tcatcgatcg caggagcaaa
      301 aagaagaatc gtccctatcg taatgaatgt tttagttatt acgtccaagt ttaaaggtcc
      361 ccctgacctg ccccagtaag ttgtaccacc ttctaggtta gaatttccat attttgcttt
      421 atcgtctcag gcgctggagg tcgataaccc agtgcactat ggggtcgaaa ttggttgtat
      481 tctttcctcc aacgctcgat caatacttgc gcttccaaga gcgaagtgaa gatctctcca
      541 ttcagtagct cgtctctcaa tttgccgttg aacgattcat tgtacccatt ctcccaggga
      601 cttcctggtt cgataaacaa ggtttgaacg cctaatcgtt ccaaccaata ccttacagct
      661 tttgctgtaa actccggacc gttgtcagac cggatgaagc ctggcagtcc tctataaata
      721 aacagatagc cgagttgctc caagacatcc tgggatcgca atcgacgggc cactttaaga
      781 gccaggcact ccctcgtata ctcatcaata agcgtcaata tccgtaaagg aagcccatcc
      841 tctgttcggg tagtcacaaa gtcataactc cacacatgat ccttgaactg aggccgcaac
      901 cgaatacaag aaccgtcatt cagccacaat cgttttctct taggctgttt ttgtggaacc
      961 tttaaacctt cccgacgcca gagacgctcc actcgtttat gatttacctt aaagccttcc
     1021 ctgcgcaata aggctgtaat cctgcggtat ccgtagcggc catactttgt cgccagctta
     1081 atcatccgct ttgtcaactg atcttcatca tctcttgtcc ttgggcgata acgctgagtg
     1141 gagcgaggct gatttaatgc ccgacaaact ctccgctctg aaacatcgaa ttgttccatc
     1201 acatgaaaca cggctcgtcg cttctttgtc gggcttaata ctttccctct gccacttcct
     1261 tcaatatcgc cttatccaat gacagatccg ctacaagctt tctcagtctg gcattctctt
     1321 tttccatttc cttcaaacgc ttggcctgat ccacgctcaa actaccgtac tccttgcgcc
     1381 aacggtaata ggtctgctcc gtgatgccta tttgacgaca aatatcggga attgtactac
     1441 ccttggaatg caaaatctcg gcttcacgta aataaccaat aatctgttct ggttgatacc
     1501 ttttcctgcc catatcccac cccctaatca tcaggttttt gatccaattc ctaatatacg
     1561 ctttggatca gttttcgggg ggcaggtcag acctttcata cctcggaagg cagttgggga
     1621 tgtttccagg catgggaact tataccaata tcgtgggata gcacaagata aagagcaagg
     1681 ctcaaactag ccgagagctt tgtattagaa cagtgccatt caccatttca tcaatgtcca
     1741 tccaaagaca atggcccaaa atagtgcggg caccccgata agtaatattg cctcaaaatg
     1801 catagctaga attcctggca gtttttattc tgataatacg ataagccttt taagagattg
     1861 tcggtatcgt ttaaagtatg ttttgtctgt cattgaatgt atgacaagaa ttccagcgta
     1921 aggaggaggg cgcaatagag atattggaag gtaattatta ttaatatgga gaaaaattgg
     1981 gcccccagaa agaa
//
LOCUS       DBNGSX010000368        10594 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1902921, whole genome shotgun sequence.
ACCESSION   DBNGSX010000368 DBNGSX010000000
VERSION     DBNGSX010000368.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10594)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10594)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10594
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1902921"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="AC16CB_11050"
     CDS             complement(<1..674)
                     /locus_tag="AC16CB_11050"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018793695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR3343050.1"
                     /translation="MSTQTITSTTTPHEQSTNYDQYTLWQILGIWALVALPMALLAWV
                     VAPAIIPYSPLHPGIIYWLLMIAGMAWQFVVSLVIVYRELGTLRWSAIRQRTWLQTPR
                     DPKTDQPNPKLYWWLLPVLLFSAVVELGLANYLEAPMAWLFPALQPAQYMDQSQLATP
                     ELQGQWWLLGIALVSILFNYFLGEEFLFRGVLLPKMQGVFGKYDWVANAVLFSFYHLH
                     RIWTLPT"
     gene            complement(746..2749)
                     /locus_tag="AC16CB_11055"
     CDS             complement(746..2749)
                     /locus_tag="AC16CB_11055"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3343051.1"
                     /translation="MYNLLVIKKSMTMMLTLVIMLSVVAPVMAQENRDTPGKNVSTTP
                     SQGQGPDDPAELEAFLDELMAKDMEEHHIPGAAVSVVKDGMLFFAKGYGYADLENRIP
                     VEPEQTVFAIGSAGKLFTWTAVMQLVEQGKLDLEADVNTYLDFHIPDTYPQPITLRHL
                     LTHTAGFEDLWFEAFAWEADDLMPVGEWLASHIPGRVRPPGEVAAYSNYGAMLAGYIV
                     ARVSGQPYEQYIQEHIFDPLRMEHSTMQRPLPPNLREHESKGYWYSDGAYQAVPVVPD
                     DYLGQMAMNPAGGHLSSAMDMARFMITHLQGGFYGDASSEIRILEETTARQMQDTLYA
                     PDPRILGTAYGFFDFSDNGQRTLGHSGSTIGFATLLLLLPDQNLGVFVVYTNEDVGGL
                     TTQHFGFQRAFFDHYFPAPSVEPIQPPADFDQRASRFVGSYRNARSAYTTFEKFFNLM
                     RPPVEISDPGDGTLLLTINGLELRLVEVEPLYFRQVDGPFGVVFREDDRGRITHMFLD
                     VSPQEDYEKMNWYETAGFNMALLLGCVLAFLSMMPVAVVRAIRNRRPSDDRKPASRGA
                     RVANWTIVGISVLNLLFVTGTVMMASGLGFPRFGLTLTDRIVLGLGVFSALLTVGALV
                     FTILAWKNRYWGNAARTYYTLVTVAAVVFVWFLNQWNWLGWRF"
     gene            complement(2784..3602)
                     /locus_tag="AC16CB_11060"
     CDS             complement(2784..3602)
                     /locus_tag="AC16CB_11060"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3343052.1"
                     /translation="MSANVSGQFQLVNEKGWRRGLGNLLQGEYSSWFKSSRWWKHLIL
                     WFSIINVMMVIMIIASAQAAKGGDEGPPILFMYGIFGGMFVAFGVMIIMQRVLVGEKR
                     SGTAAWVLSKPVTRTAFVVSRLVANSIAILLTSVIVPGVIYYITLGLFSDFGWLSPFG
                     FTAALLMISLHTFFWIALVLMMGTLSESSGGVIAVPMALYFIFWMGTGMVPGLIYISP
                     LLLTFSPDPEQMNALSVSFMTGEPVFSWLPLISTAVYCVIFIAVAIWLFNRQEF"
     gene            complement(3599..4552)
                     /locus_tag="AC16CB_11065"
     CDS             complement(3599..4552)
                     /locus_tag="AC16CB_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015121894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3343053.1"
                     /translation="MNSNDLVISTEGLSKSFGEVHALNSLNLRVPQKSIFAFLGPNGA
                     GKTTTIKLLLGLLKPTSGGGKIFGMDILHDSVDIRANIGYLPQDTRFYDHMTARQTLE
                     YTAKFFYAGPQSEIDKRVNEMIELVGLEGKADRPIKGFSGGERQRLGIAQAEVNYPDL
                     LILDEPASSLDPQGRRDVLDVMSRIRKYATIFYCTHILDDVQRVSDQVAIVNQGELVT
                     QAPIEELLAGTGDAIYSVTLRGDAHSAYTQVNQQAWVSGIEASQQGEQTTWQVSVTDE
                     AAAKDQLMSLLVSNGLKVSNFSRKEHNLEDIFINIVERSQK"
     gene            complement(4766..5578)
                     /locus_tag="AC16CB_11070"
     CDS             complement(4766..5578)
                     /locus_tag="AC16CB_11070"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR3343054.1"
                     /translation="MKTALTERNFQATAAVEVGEQYSLAKILGIWASIALPMGLIHWV
                     VSPILIPRVNMVPGFLYFIMITLGLVWQGVVAYLILRREVKPFTWENLKDRLWLHTPT
                     DPNTGVPSKRLYLWTIPLIVVINVGYAISGRLNELWVKILPFLTPPPYTEIQNLAEPA
                     VGQWWLLGVVAVMIVFNYLVGEELIFRGILLPKMNGVFGKWDFIANAILFATYHLHKI
                     WVWPQILVFNWIIPWAAKRFKSYWVAAIIHGAFDALTLIILFTMAIMGLLSG"
     gene            complement(5592..6410)
                     /locus_tag="AC16CB_11075"
     CDS             complement(5592..6410)
                     /locus_tag="AC16CB_11075"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR3343055.1"
                     /translation="MSTQEITSKDKSRVLSTHHEKSQYNLSQILWIWALASIPGALLF
                     WLGLPVLDRITDISVGYLVLIVIVTPYFWHFLLAFLILKKEGGDLDWNTIKDRLWLRT
                     TADPRTGKRNNTLWFWVIPAIAVYGITAALPFFQSINDGWARILPIHEPAQYSFDVLF
                     ENPGALVGDWGFFLAILMVSLLTVSEEIIFRGILLPKMNGVFGRADWIANGFLFAFYH
                     LEQPWTWPSKILYSTLTLALPARLFRSTWFSTAVHLSQGFYFLFLTLGLVLGLA"
     gene            complement(6558..7664)
                     /locus_tag="AC16CB_11080"
     CDS             complement(6558..7664)
                     /locus_tag="AC16CB_11080"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3343056.1"
                     /translation="MMKTIFKRTIFSIFALLAIVLGSTVIKIWPKNYFWVERNNARMP
                     VWVRGNVASGIFLVLNHGGPGSSGTAESLFEINPPNGQFDHASPLQALENEYAVVYWD
                     QRHSGMSSGNADPNDSQIEDFGEDLSLVIRELRARYHVQHLFLIGQSWGHSVALSYLT
                     LIDGWAGNQAGVDGYIIYKANNEQDIAYQAARPRIIQFANQEIAAGRDIAYWKAAIQF
                     YEQRPHLRDVTDFSANSEYADRAMQVSYPLQARIWTAFKSSIFSPLNGWAIYFNFKKT
                     IEAEGFIRYVTTDTTLRQTLPHLSIPVLLIYGAKDLQAPVEVGQSIYQDISTPESQKT
                     LLILPNSRHGAESNDVPLMQTAIKDFINQTLTLK"
     gene            complement(7677..8363)
                     /locus_tag="AC16CB_11085"
     CDS             complement(7677..8363)
                     /locus_tag="AC16CB_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343057.1"
                     /translation="MKSNTSTSQKIGWVVFTIGGLYMFGLGWLYSWQMVPAANQIGSD
                     AYSGLLGFIWALSVPLGAFIVAVGAALIARVERRVFWLLILLVVLFTVWRIAGTTNHM
                     IPALFGIGGGLITLFFLGSTWHWARIRPTLTGAGKTGSDLRMVGFIFFVVAAWDLCGI
                     FGMVNYVLRPELADKFSVPIGSTINAASTVMVLLILGWGFTFFGQLMSRRAQAVDPRV
                     DQVLQMAATD"
     gene            complement(8497..9231)
                     /locus_tag="AC16CB_11090"
     CDS             complement(8497..9231)
                     /locus_tag="AC16CB_11090"
                     /inference="COORDINATES: protein motif:HMM:NF021443.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HIR3343058.1"
                     /translation="MNKRKEKYSTVVWLALVLLGALLLAGCSSRLRVGELKTESQSVE
                     LGDAKPVNVEINFGAGDLRVNGGAEKLLEANFTYNVARLKPEVESTDDTLIVRLPEDK
                     GLPAFGDIADFRNEWDLRLNNEVPMDLSVNVGAGTSDLKLAGLSLTGLDIHLGASEST
                     VDLSGNWVRDLDITIEAGVGDVTVRLPKDIGVRVEVETAIGTMDAPGLKQDGNVYTNA
                     AYGVSDVTLHIKLEAGIGQINLEVTD"
     gene            complement(9383..>10594)
                     /locus_tag="AC16CB_11095"
     CDS             complement(9383..>10594)
                     /locus_tag="AC16CB_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343059.1"
                     /translation="LSVIAIPGLATGLWIGIFQLHGAYQSMFTFGRVYNAPLYYWAFL
                     ALAVAIATTIHVLFKRKVGATDLAFGALLLWWILAAVFSILMPGFCYLLIWPLLFSTL
                     ALGWVFWRKYPEVNSWRREMVLVAGAVPGLIILTPTIKAMSEYGPMSFLGVTFLFVAL
                     LFALLTPQLDLITRARRWRLPGVALLASLGFLIAGSLTAGFDAEHPRPTTMAYLLNAD
                     SGQATWFSPGTQLDAWTTQFYGAQPERGTMGELIPIVRRNQYPILHGEAPSVALDAPE
                     VEVLNDQTANGVRVLQLRLRSPRQAPVIYLDVEPRAAVRAVVIDGQRINTPESEGNLW
                     SLTYCAVPPEGIEITLEVESSQSLTLQVSDISMELPDIPGTAFTPRPDDMMPMPDFDY
                     GTVVVRTLEIP"
BASE COUNT         2606 a         2832 c         2759 g         2397 t
ORIGIN      
        1 gtggggagcg tccagattct atgcaggtga taaaaactga aaaggaccgc gtttgccacc
       61 caatcgtact ttccaaaaac cccttgcatc ttgggcagga gtacgccgcg gaagagaaat
      121 tcctcgccca ggaaatagtt gaagaggatg ctcaccaggg caattcccag gagccaccat
      181 tggccctgga gttcgggggt agccaactga gattgatcca tgtactgggc aggctgaaga
      241 gccggaaaca gccaggccat gggcgcttcc agataattgg caagccctaa ctccaccacg
      301 gcagagaaga gcaagacagg tagcagccac caatatagtt tcggattagg ttgatcagtc
      361 tttgggtcgc gcggggtttg cagccaggtg cgctggcgga tggcgctcca gcgtagggtg
      421 ccgagctcgc ggtaaacgat caccaaagat acgacaaact gccatgccat accggcgatc
      481 atcaacagcc aatagatgat gccgggatgc agcgggctgt aggggatgat ggcgggagcg
      541 accacccaag ccagcagcgc catgggcagg gcaaccagtg cccaaatgcc caggatctgc
      601 cagagggtgt actggtcgta attcgttgac tgctcatgcg gggtagtggt tgaggtgatt
      661 gtttgagttg acatgagatt tctcctttta ttattggctg gcgggtgatg aactacatcc
      721 ccgccagcct cgaaacgttt atcagttaga accgccagcc caaccagttc cattggttca
      781 ggaaccagac aaaaaccacc gccgccaccg tcaccagtgt gtaataggtg cgggctgcgt
      841 tgccccagta gcggttcttc caggccagta tagtgaagac cagcgcgccg accgtcagca
      901 gggctgaaaa aacgcccagt cctaacacga tcctgtcggt caatgtgagg ccgaaccttg
      961 ggaatcccag gccgctcgcc atcatcactg ttccagtaac gaacagcagg ttcaaaacgc
     1021 tgatccccac gatcgtccaa ttggcgacgc gggcgccgcg agaggcgggt ttccgatcat
     1081 cgctggggcg gcggttccga atcgcacgga ccactgcgac gggcatcatg gacaggaagg
     1141 ccaagacaca tccgaggagc aatgccatat tgaaaccggc ggtttcgtac cagttcattt
     1201 tttcgtaatc ctcctggggc gagacgtcga gaaacatgtg ggtgatccga ccccggtcgt
     1261 cctcacgaaa aacaacaccg aatggaccgt ctacctggcg gaagtacaac ggttccactt
     1321 caactaaccg caactcgagc ccatttatgg tcaatagcag tgtgccgtca ccgggatcgc
     1381 taatttcaac gggtggtctc atgaggttaa agaatttttc gaacgttgta tatgcgctcc
     1441 gagcgtttct atatgaaccc acgaaccggc tggcacgctg atcgaaatcc gcgggaggtt
     1501 ggattggctc aacagagggt gcgggaaagt aatggtcgaa aaacgctctc tggaacccga
     1561 aatgctgcgt agtcagtccg ccaacatcct cgttggtata gaccacaaag acacccaggt
     1621 tctggtcggg taaaagcagc aacagggttg cgaaaccgat cgtgctgccg ctatgcccga
     1681 gcgtgcgctg gccattgtcg ctgaaatcga aaaagccgta agcggtgccc aggatgcgcg
     1741 ggtcgggagc gtacaatgtg tcctgcattt gccgcgccgt ggtttcctcc aggatgcgta
     1801 tctcggagga tgcgtcgccg tagaagccgc cctgcaggtg ggtgatcatg aaacgcgcca
     1861 tgtccatggc actgctcaaa tgtccgccag cgggattcat cgccatctga cccaggtagt
     1921 catcggggac cacagggact gcttgatagg cgccatcgga ataccagtat cccttggact
     1981 catgctcgcg caggtttggc ggtaacggcc tctgcatcgt cgaatgctcc atgcgcagcg
     2041 ggtcaaagat atgttcctgg atatactgct catagggctg tcctgagacc cgcgccacga
     2101 tataccccgc cagcattgca ccgtaattcg aatatgccgc gacctcgcca ggtgggcgta
     2161 cacgtccagg tatgtgggaa gccagccatt cgcctaccgg catcaggtca tcagcttccc
     2221 acgcaaaagc ctcaaaccac aggtcttcga aacccgccgt atgggtcaaa agatgcctga
     2281 gcgtgatcgg ttgtgggtat gtatctggaa tgtgaaagtc gagataggtg ttaacatccg
     2341 cctccaggtc cagcttgccc tgctcgacga gctgcatcac tgccgtccag gtgaatagct
     2401 tgccggctga accaatggca aagactgtct gttcgggctc gacagggatg cggttttcca
     2461 ggtcggcgta gccgtagcct ttggcaaaaa acagcatccc gtctttgacg acagacacgg
     2521 cggctcccgg gatgtggtgc tcttccatat cctttgccat gagctcatcc aggaatgcct
     2581 ccagttcggc ggggtcgtca gggccttgtc cttgggaggg cgtggtagat acattcttac
     2641 ccggcgtgtc cctgttttct tgggccatga ctggtgcgac aaccgacagc attatcacga
     2701 gagttaacat catcgtcatg gatttcttga taacgagcag gttgtacatt ttccttctcc
     2761 tatacgaata tttcgcaaga tcattaaaac tcctggcggt tgaagagcca gatggctacg
     2821 gcgataaaaa tgacgcaata gactgccgtc gagatcaacg gcagccatga gaacaccggc
     2881 tcaccggtca taaaagaaac agacagggca ttcatttgtt ctggatcagg gctaaaggtc
     2941 agcagcaatg gcgagatata gatcaggccg gggaccatac ccgtccccat ccagaatata
     3001 aagtacaggg ccatcggtac ggcgataaca ccccccgatg attcagaaag ggttcccatc
     3061 atcagtacca atgcgatcca aaagaatgtg tggagtgaaa tcatcaatag ggcggcagta
     3121 aatccgaagg gagaaagcca cccaaagtca ctaaataatc ccagggtgat atagtagatc
     3181 acgccaggca cgatcaccga ggtgagcagg atggcgatcg aattggcaac caaccgggag
     3241 acgacaaaag ccgtgcgcgt gaccggtttg gagagcaccc aggccgccgt gccggagcgc
     3301 ttttcaccga ccaggacccg ctgcatgatg atcatcaccc cgaaggcgac aaacatcccg
     3361 ccaaagatgc catacatgaa taggattggc gggccctcat ccccaccttt ggctgcttgg
     3421 gcagacgcaa tgatcatgat gaccatcatc acgttgatga tcgaaaacca caggatgaga
     3481 tgtttccacc atctggagga tttgaaccat gacgaatact cgccctgcaa caagttaccc
     3541 aacccccgcc tccagccttt ttcattgaca agttggaact gccccgacac attggcgctc
     3601 atttctggct cctttcaacg atattgatga agatatcttc caggttgtgt tctttgcggc
     3661 tgaagttgga gaccttcaaa ccgttggata ccagcaggct cattaactgg tctttagccg
     3721 cggcttcgtc cgtcacgctc acctgccagg tggtctgttc gccctgttgg ctggcctcga
     3781 tcccggaaac ccaggcttgc tggttgacct gggtgtaggc gctgtgggca tcgcctctca
     3841 gggttacgga gtagatcgca tcacctgtac ctgccagcag ttcttcgata ggtgcctggg
     3901 tgaccagttc accctggttg acgatcgcaa cctggtcgct gaccctttgt acgtcgtcca
     3961 ggatatgggt gcagtagaag atcgtggcgt atttgcggat gcgggacatc acatcgagca
     4021 cgtctcgccg tccctgcgga tcgaggcttg aggcaggttc atccaggatc aataggtccg
     4081 gatagttgac ctccgcctgg gcgatgccca accgctggcg ctcacctccg gagaaaccct
     4141 tgatcggccg gtctgccttg ccctccagtc caaccagttc gatcatctca ttcacgcgct
     4201 tgtcgatctc cgattgaggt ccggcataga agaacttagc agtgtattcc agagtctggc
     4261 gggcggtcat gtgatcgtag aaacgggtat cttgaggtag gtagccgatg ttggcgcgga
     4321 tatcgacgct atcgtggagg atatccatgc caaagatttt gccgcccccg ctggtaggtt
     4381 tgagcagccc cagcaacagc ttgatcgtgg tggttttgcc agcaccgtta ggcccaagga
     4441 aggcgaagat cgatttctga gggacacgca ggttgagcga attgagcgca tgcacctcgc
     4501 caaaagactt gctcaaacct tccgtgctga tgactaagtc atttgagttc atgttgcttt
     4561 ctccttttct tcaagtgaga ctatctcgat tacattctat aacccaagca aaaaaaaaga
     4621 accacatgat catgtggttc taagacaggt aaatatgtgg ttttgccgcc aggtgcagat
     4681 caaattcatc tctcggtcat gacgccgccg caccatatgg ttgatcctcc actcccaggt
     4741 cgccagctgc attagccgat caatcttaac cgctcaacaa tcccatgatt gccatagtga
     4801 aaaggatgat cagtgttaaa gcgtcaaacg ccccgtggat gatggccgcc acccagtacg
     4861 acttgaaccg ttttgccgcc cagggtatga tccaattgaa gacgaggatt tgcggccata
     4921 cccagatctt atggaggtgg tatgtcgcga acagaatggc gttggcgata aagtcccact
     4981 tcccgaacac gccgttcatt tttggcaaca agattccccg gaagatcaat tcctctccca
     5041 ccaggtaatt gaacacgatc atcaccgcga cgacacccaa caaccaccat tgtcccacgg
     5101 ctggctcagc cagattctgg atctccgtat aaggaggcgg cgtcaaaaat ggaaggatct
     5161 tcacccagag ttcgttcaac ctcccagaga ttgcatatcc cacattaata accacgatca
     5221 atgggatcgt ccagaggtac agcctttttg atggcacgcc tgtattggga tcggtggggg
     5281 tatgtagcca caatctgtct ttgaggtttt cccaggtgaa cggtttcact tcccgcctga
     5341 ggatgaggta agcaacaacc ccctgccaga ccaaacctaa tgtaatcata atgaagtaca
     5401 ggaatcccgg aaccatgttc acacgtggga tcaggattgg actgaccacc caatggatga
     5461 gtcccatggg cagggcaatg cttgcccaga tgcccaggat tttggcgaga gaatactgtt
     5521 cacctacctc aacggctgca gttgcttgga aatttctttc tgtgagtgct gttttcatgt
     5581 tttctcctta tttacgcaag acccaataca agccccaagg ttagaaaaag aaagtaaaat
     5641 ccctggctaa gatgcacagc ggtcgagaac caggtgctcc ggaataatcg agccggcagg
     5701 gccaaagtca gggtggaata caaaattttg cttggccaag tccagggctg ttccaaatga
     5761 tagaaagcaa ataggaagcc gttggcaatc caatctgctc ggccaaatac tccgttcatt
     5821 ttcggaagca aaatcccccg aaatatgatc tcttccgaca ccgtcaatag actgaccata
     5881 agaatggcga ggaaaaatcc ccaatcaccg acgagcgccc ccggattttc aaacagcaca
     5941 tcaaagctgt attgtgcagg ttcgtggatc ggtagtattc gtgcccaccc atcattgatg
     6001 gattgaaaga atgggagtgc agccgtgatc ccataaaccg cgatcgctgg aatcacccaa
     6061 aaccagagtg tgttgtttcg ttttccagtt cttggatcgg ccgtcgttct cagccataat
     6121 cgatccttga tggtattcca atccaggtca ccaccttcct tcttcaggat caaaaaggcc
     6181 agcaaaaagt gccagaagta tggggtgaca atgacgatca gcaccagata gccgacactg
     6241 atgtcagtaa ttcgatccag gaccggtaaa cccagccaaa aaagcagggc accaggaatt
     6301 gaagcaagcg cccatatcca taggatctgt gaaaggttgt actgggattt ctcgtggtgt
     6361 gtagaaagca ctcgcgattt atcctttgat gttatttctt gagtagacat gagatttctc
     6421 tttttttact tttaggttat ctgaacaaag gttgtccgtt cgtttatctg atatttccac
     6481 tagatatctc ctttgggatt aaaacccctg gcggttgaag cgccagatag ctacaaagat
     6541 gaaaaagaag cgatgtttta cttcaacgtc aaagtttgat taataaagtc cttgatagct
     6601 gtctgcatta acggaacatc attgctttcg gcgccatgcc gggaattggg cagtatcagg
     6661 agagttttct gtgactcagg tgtggagata tcctgataga tgctttgccc aacttctact
     6721 ggcgcttgga gatcttttgc accataaatc aggagcaccg ggattgacaa atgtggtagt
     6781 gtttggcgca gagtcgtgtc ggtggttacg tatcttatga atccttcggc ttcgatggtt
     6841 tttttgaagt tgaagtagat tgcccaacca ttaaggggtg agaagataga tgatttaaaa
     6901 gcggtccaaa tacgcgcttg aagtggataa gacacttgca tagcccgatc agcgtattcg
     6961 ctgttggcgc taaaatcggt gacatcacgc agatggggtc tttgctcata aaattgaatt
     7021 gcagccttcc aataggctat gtcacgccca gcggcaattt cctggttggc aaactggatg
     7081 atccgtggtc gggcagcctg atacgcgatg tcttgttcat tgttggcttt gtaaataata
     7141 tagccgtcta ctccagcctg attgcctgcc caaccatcga ttagcgtcag gtaactcaat
     7201 gcaacactat gtccccagct ctggccgatc aggaaaagat gctgcacatg ataacgtgcc
     7261 ctcagttcgc gaattaccag agacaaatct tcgccaaaat cctcgatctg gctgtcattg
     7321 ggatctgcat tcccgctcga catccctgaa tggcgctgat cccaatacac cacggcgtac
     7381 tcgttctcga gcgcttgcag gggagaggca tggtcaaact ggccattagg agggttaatt
     7441 tcgaaaaggg attctgctgt gccggaacta cctgggccac catgatttaa tacgagaaag
     7501 atgcccgagg ctacatttcc cctgacccat accggcatgc gggcgttatt acgctcgacc
     7561 cagaaatagt tttttggcca gatctttatt acggtcgaac ctagcacgat agccagcagt
     7621 gcaaatatcg agaagatagt tctcttgaaa attgtcttca tcatgggtta tttcctctaa
     7681 tcagtcgcag ccatctgaag aacctgatcc actcgaggat caaccgcctg tgcccggcga
     7741 gacatcagtt gaccgaagaa ggtgaatccc caacccagaa tcagcaagac catcacagtt
     7801 gatgcggcgt tgatcgtgct gccgatcggc acagagaact tgtccgccag ctcgggtctc
     7861 aaaacgtaat tgaccatacc aaatatgccg cacagatccc aggcagccac aacgaagaag
     7921 ataaatccga ccatgcgcaa gtccgatcct gttttgccag cccctgttag cgtgggacgg
     7981 atcctggccc aatgccaggt cgacccgagg aaaaacaagg tgatcaagcc cccaccaatc
     8041 ccaaagagcg ctggaatcat gtggttggtt gtgccggcta ttctccagac cgtaaaaagc
     8101 acaaccaaca ggatcagcaa ccagaataca cggcgctcaa ccctggcgat cagggctgct
     8161 cctacggcga cgatgaatgc tcctagcggt acagatagag cccagataaa gcccaataac
     8221 ccagaatagg catcgctgcc aatctgattg gctgcgggca ccatctgcca cgaatacaac
     8281 cagcccaagc caaacatata cagcccaccg atcgtaaata ccacccatcc gattttttgc
     8341 gatgtggatg tattgctttt catgactcac tcctttgact tcaaacttac ttgtttctct
     8401 cttgatacga tttttagccg atctcaactt gattcactgc acgtgcattt aactcttgat
     8461 ggctctatgc aggccaatgc caagagttgc gcctgcctaa tctgttactt ccaggttgat
     8521 ctggccgatg ccggcctcca gttttatatg caaagtcaca tctgatacac cataggcggc
     8581 gttggtgtaa acatttccat cctgtttcag gccgggggca tccatcgtgc ctatagcagt
     8641 ctcgacctcg acgcgaactc cgatgtcttt aggcagtcgc acggtgacgt cacccacgcc
     8701 ggcctcgatg gtgatatcaa gatcacggac ccagtttccg ctcaggtcga ccgtgctttc
     8761 gctcgcacca agatggatat cgagcccggt taacgacagg cctgccagtt ttaaatcgct
     8821 ggtcccggcg cctacattta cgctcagatc catgggcacc tcattgttaa ggcgcagatc
     8881 ccactcatta cggaagtctg cgatgtctcc aaaggctggc agacctttgt cttcgggcag
     8941 ccgcacaatg agcgtatcgt ccgtggactc aacctctggc ttcagcctgg cgacgttata
     9001 ggtgaaattg gcctccagca gcttttccgc gccgccattc acacgcaggt cgccagcgcc
     9061 gaagtttatc tcgacgttga ccggcttggc gtctcccagc tcgaccgatt gagattcagt
     9121 cttcaactcg cccaccctta gcctggaact gcagccggcg agtagcagcg caccaaggag
     9181 cacgagagcc agccaaacaa cggtgctata cttttctttt cgtttattca tttcaaaacc
     9241 tccgttgagt ttcaatgatt gacatttccg actttgttct aaagggatct atttcgttat
     9301 tggtattcta ttggcgcagg gaaaaaaaag aaccacatga tcatgtggtt ctctggttgg
     9361 taaatatgtg gttttgttct tactaaggaa tctcaagggt tctgaccact acagtcccat
     9421 agtcaaagtc gggcatcggc atcatatcgt ccggacgtgg cgtgaaggcc gtgccaggga
     9481 tgtccggcag ttccatagag atgtcactta cctggagggt gagcgattgg gatgactcaa
     9541 cctcaagtgt gatttcaatc ccttcgggcg gaacggcaca gtaagtcagg ctccatagat
     9601 tcccttctga ctccggggtg tttatccgct gcccatcaat gactacggcg cgtactgccg
     9661 cccgtggttc cacatccagg taaatgacag gagcctgccg cggtgaacga agccgcaatt
     9721 gcaacacccg cacaccattc gcagtctgat cgttgagtac ctccacttcc ggcgcgtcca
     9781 gcgctacaga tggagcctca ccatgcagaa tcggatactg gtttctccgg acaatgggga
     9841 tcagttcgcc catggtgccg cgttcaggtt gcgcgccata gaactgtgtg gtccatgcgt
     9901 ccagctgtgt tccggggctg aaccaggtgg cctgcccaga atccgcattc aggagatatg
     9961 ccatggtggt tggccgggga tgctcagcgt cgaagcctgc ggtcaggctg ccagcgatca
    10021 ggaaacccag gctggccaac agcgccacgc cgggcaaccg ccatctgcgc gcccgggtta
    10081 tcaggtcgag ttgaggggtg agcaatgcga agagcagcgc gacgaacaag aaggtgacgc
    10141 caagaaagga catggggcca tattcggaca ttgccttaat ggttggggtg aggatgatga
    10201 gtccaggcac agcgccagcg accagtacca tctcgcgtcg ccaggaattt acttcagggt
    10261 actttctcca gaaaacccaa cctagggcca gggtgctgaa cagcaacggc cagatgagca
    10321 ggtagcaaaa acccggcatt agaatgctga acaccgccgc caggatccac cacaacagca
    10381 gcgccccgaa cgccaggtca gtggccccaa ctttccgctt gaaaagaacg tgaatggttg
    10441 tcgcgatggc cacagccagg gcgagaaagg cccaatagta caggggagcg ttatagactc
    10501 tcccaaaggt aaacatagac tggtatgctc cgtgcagttg gaagatccct atccacaggc
    10561 cggtggccag gccgggaatt gcaatgacac tcaa
//
LOCUS       DBNGSX010000369         2451 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1003138, whole genome shotgun sequence.
ACCESSION   DBNGSX010000369 DBNGSX010000000
VERSION     DBNGSX010000369.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1003138"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="AC16CB_11100"
     CDS             complement(<1..437)
                     /locus_tag="AC16CB_11100"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HIR3343060.1"
                     /translation="MLTNIWWIRRDLRLADNLALNEARKGAGALVPLFILDPHLLKQP
                     APSRKAFLFEGLRRLDEDLQRMGNRLVVRSGDPLDVLRQVVAESGAQRIYAVEDYSPY
                     ALQRDEHIDRVLPLTLQPGVTVRHPASVAKPDGSPYTVYTPFS"
     gene            614..2017
                     /locus_tag="AC16CB_11105"
     CDS             614..2017
                     /locus_tag="AC16CB_11105"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3343061.1"
                     /translation="MPQLMRCLYCGLLQDEPAGVKSCARCGGELGYESMPTSDERSSY
                     VSVQMELDQITAPSRQNCDRYLMVTLRTPAQVPAEQAAPTQSGRPPLNFCAILDVSGS
                     MQGEKITQAKEAVRQTLNHLREGDVFSLVTFSSDVRAVFEPRLVDSQTRRVVESALQE
                     IQAGGMTALDGGLALGLEKIASRKEETNLALLLSDGQANVGETDLEKVGQRGYAARQQ
                     GVIVSTLGVGLDYNEALMVEIATQGGGRFYHLHSAGQIAVFLAGELGEVTSLAARETT
                     LQLDLPPGATLVPLSSAYLAEQSGGRASVVIGDIPSDTELEIPLRLTLPAQSPGTKLS
                     IEGSLQYRSPAGNLLHTKLNRVTVRFVKKAIFKLRDGIVLPVAERVFEQMKAASVLRV
                     SRAYAMNAVDGQRQSEERLAAMHEYAVKLGDERAVEEMKIAEQSFGFMASSPSAAKAA
                     VADAFSKQRSSKDFDKK"
BASE COUNT          574 a          694 c          678 g          505 t
ORIGIN      
        1 ttgctaaatg gggtatacac cgtatagggg ctgccatctg gctttgccac tgaggcggga
       61 tggcgaaccg tcacgcccgg ctgcaaagtc aacggcaaaa cccggtcaat gtgctcatcg
      121 cgttggaggg cataagggct gtaatcttcc accgcgtata tgcgctgtgc accgctctcc
      181 gccaccacct gccgcaggac atccaagggg tcgccagagc gcaccaccag gcgattgccc
      241 atgcgctgca aatcctcatc caaacggcgc aacccttcga agagaaacgc cttacgcgat
      301 ggtgccggct gcttgagcaa atgcggatca agaataaata gcgggactag cgctcccgct
      361 ccttttcgtg cctcgttcaa agccagattg tccgccaggc gcaggtcacg gcggatccac
      421 cagatgtttg taagcatgtc tgcgaacctc tacttctcga tttctaaaga ttatagctga
      481 taggctttat cgtatccagc ttgagcaagt attgatccag cgtaataaca ctaatacgat
      541 agctctaggt ttagattcac ctcttgctga attgcaagaa cgttctataa tcatatacac
      601 aaaggaggaa ctcatgccac aactcatgcg ctgcctgtat tgtgggctcc tgcaggatga
      661 gcctgccggt gtgaaatcct gcgctcgatg cggcggggag ctggggtacg aatcaatgcc
      721 aacttcggac gaacgaagct cctatgtttc cgtccagatg gagctagacc agatcaccgc
      781 gccttccagg caaaattgcg atcgctatct aatggtaaca ctgcgcacgc ccgcacaggt
      841 gcccgcggag caggccgcgc ctactcagag cgggcggccc cccttaaact tctgcgccat
      901 actggacgtc tccggctcaa tgcaaggcga gaagatcacc caggccaagg aagccgtgcg
      961 ccaaacattg aatcacttgc gtgaaggaga tgtgttctcg ctggtaactt tttcatccga
     1021 cgtgcgtgcc gttttcgagc cacgcctggt agattcccag acccggcgtg tggtggagag
     1081 tgccctgcag gagattcaag ctggcggcat gaccgcactg gatggtggat tagcactcgg
     1141 actagagaaa atcgcttctc gaaaagagga gaccaacctg gcattgctgc tgagcgacgg
     1201 acaggccaat gttggtgaaa cggacttaga aaaggtcggg cagcgaggct atgcagcccg
     1261 gcaacaaggc gttatcgtat ccacgctggg tgtggggcta gactacaatg aggcattgat
     1321 ggttgaaatc gccactcagg gaggcgggcg tttttaccac ctgcattccg ctggacagat
     1381 cgccgtcttc ctggcaggtg agctgggcga ggtgacttcc ctggctgccc gcgagacaac
     1441 cctgcaatta gatttacccc ctggtgccac gttggttccg ctttcgtcgg cctaccttgc
     1501 tgagcaaagc ggagggcgag catctgtcgt catcggtgat atccccagcg acaccgaatt
     1561 ggagattccc ctgcgcctga ccctgcccgc ccaatcccct gggaccaagc tcagcatcga
     1621 aggctcactc caataccgct cccctgcggg caacctgctc cacaccaagc tgaaccgggt
     1681 gacggttcgc ttcgtgaaaa aggctatctt caaattgcga gatggcatcg tgctgcctgt
     1741 ggctgagcgg gtctttgagc agatgaaggc cgccagtgtg ctgcgggtct cccgcgccta
     1801 cgccatgaat gctgtggatg gccagcgaca atccgaagaa cggttggcgg cgatgcacga
     1861 gtacgctgtc aagctgggag acgagcgggc agttgaagag atgaagatag cagagcaatc
     1921 tttcggtttt atggcgagca gcccgagcgc agccaaagct gcggtggcgg atgccttctc
     1981 aaaacagcgc agctcgaagg attttgataa aaagtaaatc gctaataaac atgaaccctc
     2041 ccgaactttg tcttgcgggt tgagttgtgc caagaaatac atccacccca accctacccc
     2101 gcgaacaggg gaggaagatt gatcgaaaaa acataaccca tcccgaattt aatctaacgg
     2161 actgatttgt accggttatg acccccaccc acccctaacc cccttcgggc tacgcccgca
     2221 gggcgatgcc gccatccccc actgggaggg cggggaatat atcggaaaaa cgggctattg
     2281 ggcggcggaa ccgccgccca atagcccgtt tttccgtaaa tcatccccct cccgccgcta
     2341 atgcggaagg gagggggaac gagggggtgg gcaaacaggt tacgagacac aacaaacata
     2401 aagtttggtg tgtttcatac tattctctct tcttcagcaa gctaacgtcc t
//
LOCUS       DBNGSX010000370        13377 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1071990, whole genome shotgun sequence.
ACCESSION   DBNGSX010000370 DBNGSX010000000
VERSION     DBNGSX010000370.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13377)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13377)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13377
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1071990"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="AC16CB_11110"
     CDS             <1..202
                     /locus_tag="AC16CB_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343062.1"
                     /translation="GWPVCTMLCMPQPGFDLVCRYFGAQYATFTFRILVKEPKSSPNT
                     LFTGLRMPLRAYAIALVDAPWS"
     gene            527..1675
                     /locus_tag="AC16CB_11115"
     CDS             527..1675
                     /locus_tag="AC16CB_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3343063.1"
                     /translation="MIERKVLQETIQNALDRSRVVALIGPRQCGKTTMARLFVPSTSI
                     NYFDLEDPLSLTRLDQPMTALQDLRGLVVIDEVQRRPDLFPILRVLVDRTPLPARFLI
                     LGSASPDLLRQPSESLAGRISTITMSGFSLAEVGKEAQNQLWRRGGFPLSFTADTEET
                     SLEWRKDFVRTFLERDIPQFGFNIPSTSLFRFWSLLAHYHGQIWNNAEAARTLNFGEA
                     TARRYLDLLQDLFMVRLLQPWYANLSKRQVKTPKIYFRDSGLLHHLLGIRSEKDLLLH
                     PRSGASWEGFAIEETVKAVMPDEVYFWATHSGAELDLLLIKNGQRIGAEYKRVDAPRL
                     TPSMRTACKDLELVRLLVIYPGLKPYPLDENIQAIPLSSLAEMPVNLC"
     gene            2263..2535
                     /locus_tag="AC16CB_11120"
     CDS             2263..2535
                     /locus_tag="AC16CB_11120"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR3343064.1"
                     /translation="MGSLQSRGAPDGFSLPGVPWTSFFVPETGVALHGKFWHKNFGVQ
                     MSHGRVNMRNDNAKWVFRRSAAVWEIPVQDRFAWDRRGYGTCVDVH"
     gene            2704..3252
                     /locus_tag="AC16CB_11125"
                     /pseudo
     CDS             2704..3252
                     /locus_tag="AC16CB_11125"
                     /inference="COORDINATES: protein motif:HMM:NF017539.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop domain-containing protein"
     gene            3286..5505
                     /locus_tag="AC16CB_11130"
     CDS             3286..5505
                     /locus_tag="AC16CB_11130"
                     /inference="COORDINATES: protein motif:HMM:NF037660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3343065.1"
                     /translation="MGEEIRDFLDQIMSLHLPEPQIAALESKTEGWIAGLLLAAHSLQ
                     YEPESSKFVEAFAGTDRQITDYLIEEVLARLPQEIHEFLLYTSVLDRLTASLCQRLVY
                     GEGTAEQSQEILEWLERNNLFTFPLDNRRFWYRYHLLFAEFLRYRLALVSPEKVFDLH
                     LKASEWMEANGFVTEAINHALVGENNRRALDLIENHGLAAISKAEVRKVQRWFENLSK
                     EMIRSRSSLSILYAWTLLFTNYSHPPKEIDEWIQAAEEALSMQEGEPDKAKTDKEHKV
                     REHIYAIRPILAFSRHEDPQAVIESGKRALDFIDESNSWLRSILLHSIGAAHIIIGDI
                     ESTRRFDLDAQHHAKISGMDYMGIGLYYDMVFIALRQGRLREAESVCREWLDYAHRQG
                     KMESATAGFLYALLGKILVERNDLDSAQTALAKGMDLLPLSGEDELRDLCLVDLCRLY
                     QARGEWSSSERALSENSIDSAGERSAWTESFLSALKALLWLREAEYTQSRLRLVFQWL
                     EKYAPDLGADTEYPILFPTSERDYIVQMITARIHIAYARTLPPTYREKSIQSVLSFLN
                     GQHQIAQKRGWGERVMELEIIRALALETIGELDGAFDALSRALTIGESEGYIRIFVDE
                     GHVMAKLLYEAASREIYPEYVGKLLATYPKEKPKLSLAMGDEDEEFDQVEPLSERELE
                     VLDLIAQGLSNKEISQKLYISLNTVKGHTSNIYGKLVVKNRAQAVIRARRIGLLQGE"
     gene            5974..6213
                     /locus_tag="AC16CB_11135"
     CDS             5974..6213
                     /locus_tag="AC16CB_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343066.1"
                     /translation="MDKQRIYQIRVKGQLGEKQWDWLEDMETSVSQDEGFGTITAITG
                     LVEDQALLHGILYRLYSVGYVLISVNCLDEGGRKS"
     gene            6272..6655
                     /locus_tag="AC16CB_11140"
     CDS             6272..6655
                     /locus_tag="AC16CB_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343067.1"
                     /translation="MDEENPLITLHEDWATAIDRVEQALASAGFEVIPSFDLQVAQAE
                     AGNFICPIHSNGRCDGQMIELLVYDRDNPPLTLEVLGHNGLVRFAIVDAPQRQSNPLL
                     RAVILQALREMSISKQYPGVGNHDG"
     gene            6645..7007
                     /locus_tag="AC16CB_11145"
     CDS             6645..7007
                     /locus_tag="AC16CB_11145"
                     /inference="COORDINATES: protein motif:HMM:NF014624.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HIR3343068.1"
                     /translation="MTDNRKRILWRLRTVQGHLRAIGDLFEAGETCEEILIQLKAVRG
                     AIDAIAAQLFYDQVERSKQVICEEPSLNRRSEELERLVKLYSAWKQSSFINYNTVSKG
                     ACSRAAAFPTKLQFFEDI"
     gene            7031..9559
                     /locus_tag="AC16CB_11150"
     CDS             7031..9559
                     /locus_tag="AC16CB_11150"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012448869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140358 - P-type transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR3343069.1"
                     /translation="MDQSIEFTMPVSGMTCASCAANIENALNGVEGVAQASVNLATER
                     ASLRLSKPEVKTIDLIQAVRDVGYDVPSETLTLPIGGMTCASCVSTVEGALKGVPGVV
                     DATVNLATEKATVTFVPTLATIKDFKVAVAEVGYQVLDFKEGAGQVAEDESERKMQES
                     RFRMWVAWSFTAPIILWMFLEMFFGIMWPNESIFNLGMVVLGLPVLFWVGRHTYRSGF
                     KAGIRGKPNMDTLIALGTGVSLLTGPASFFFPVANYAGVAAMIMAFHLTGRYVEETAK
                     GRASQAIRKLLELGAKTAWVLKDGKEVEVPIEDVDVGDIMIVRPGDKIPTDGVVLQGD
                     SAVDESMATGESMPVTKRQGDEVIGATINQEGLLKVKATRVGKDTFLSQVIKLVEECQ
                     GSKVPIQEFADKVTGIFVPIVLVIAILTFAAWILISAPMQALVNTGSFLPWVNPNLGV
                     ITLAIVSTVAVLVIACPCALGLATPTALMVGSGKGAEHGILIRTGEAVQTIKDVRIIA
                     FDKTGTITKGRPEVTDILPAEGFHQDEVLRLAASVEVGSEHPLGRCIVEKSRVLELIL
                     EEPQAFLAIRGKGVSATLDSGSVLVGSRRLMLEHGIDPAPLEKTLQQLENEAKTAMLV
                     AAGGRLAGVIAVADTLKEDSIDAIRELHHMGLETAMITGDNRRTAEAIARRVGIDHVL
                     AEVLPDGKVAEVQKLQDRFGLVAFVGDGINDAPALTQANVGIAIGTGTDIAIEASDIT
                     LVRGELTGIVEAINLSNATFQKIKQNLFWAFFYNVAMIPLAIIGWMHPVLAEIAMATS
                     SITVVSNANRLRSVDIRPSYLRGEKVSKRVQESSYSSKEMVVKA"
     gene            9619..11025
                     /locus_tag="AC16CB_11155"
     CDS             9619..11025
                     /locus_tag="AC16CB_11155"
                     /inference="COORDINATES: protein motif:HMM:NF014296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HIR3343070.1"
                     /translation="MSTMRRFRAPLLPIDSPTGRERALIVIERLIKGALFKCSMCGNC
                     LLQETAFICPMLCPKGLRNGPCGSGAADGCCVDPSRPCVWHLIYQRAEAKGSLDKLLE
                     VQAPLDWTRVGHETWGTVLAEARSRGLFSPSFLAHADEWRGEVTELFRGIRQPEWWQG
                     DDQYHPPRSNGAASRLQAALDNGEFVLTAEVTPPADSNTEEVLQAAVGFRDVVHAANV
                     TQNPMATTRMSSLACSALLMEAGVEPVLQLTARDHNRFTLQSEALGATAFGISNILCL
                     TGDPPTKSRGPAAGLPFDLDATQMLWILRRMRDEAQLLDSRPLKKPPILFLGTAASPN
                     DPIPQHEARRLEKKINAGAQFIQTQLVYDTARLKRWLAALAERDLLQKVHILVGVGPL
                     RSVRMARYMREHIPDISIPPGLVDRMERSPDPKETGLEIALELVDQIKDLPGVSGIHL
                     MSLGWKSILPRLLKEARL"
     gene            11147..12565
                     /locus_tag="AC16CB_11160"
     CDS             11147..12565
                     /locus_tag="AC16CB_11160"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3343071.1"
                     /translation="MMKFTVSEIFQQSRPPDATSPARSTKRLSLARLAPSRGLSLLLF
                     SSAISEVACNIVYIVLLERAYLLGRGSSSIGILMIIQSTAPVFFGSWMGGLADQLRFK
                     RSAVIANSAIAALVFSLTFAKIVTFVYIIAFLIMLGRLLLIPARLGLVAQLTDRARLM
                     EANTTLSILSGVGSFIGPAIAGMLFLASDDFAPALLGAGGGWLLSLLPLTMIQMKPGN
                     PIALRRTFPIEEIRNGWHLIRKRSTITKVLWCLIVATLLLGAVTPLITPLSRQFGLGS
                     EGTGVFFSALGFGTLIGPLIATGLFKRLHLSTTLLIVGLLAPVGLIVVGMLNHLQGVL
                     IAIAVAATAGTGLNVIVTTVTQRLSRSSHHGTVFGTQQTLLGIAWILALAATTGLTAL
                     WELESKVRMLFIALGGSGFLAILSCWTWNRQSIRKTCDLCEPELRIASVACRIVHGPP
                     YQISATLCGIICGEGCGRCYSQ"
     gene            13082..13222
                     /locus_tag="AC16CB_11165"
     CDS             13082..13222
                     /locus_tag="AC16CB_11165"
                     /inference="COORDINATES: protein motif:HMM:NF016811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HIR3343072.1"
                     /translation="MMVNENTAPSSEYKGKKYYFMNPTHKALFDKDPEKFLSRQLNRS
                     DH"
BASE COUNT         3330 a         3304 c         3449 g         3294 t
ORIGIN      
        1 tggttggccg gtttgcacaa tgctgtgcat gccgcagcca ggatttgacc tggtttgtcg
       61 ctattttggg gctcaatatg caacctttac ctttcggatt ttggttaaag agccaaagtc
      121 cagcccaaac actcttttta cgggattgcg catgcccctc agggcatatg caattgccct
      181 ggtggatgcc ccctggagtt gatgggatta ccatcaccta gatgtctact tggtgactca
      241 gctgctgcgt gcccaacacg acgcaagcat cccgtcttac atcgtatcga agttttggtg
      301 aagcaggcgc tagaggcgca gagtggtctg tcttatgctc tctcgaactg gcagctaggg
      361 gtttcggatg ttttggcata tttaccaaga atagccctat gtatctgtgg aagagatgct
      421 ccacgcctac atggtcatat tgacaacaac ccatctcaag aatacagaac atcattacca
      481 tgaaaatccg ccatcgaatg gcggattttc atggtatcat aagtccatga ttgaaagaaa
      541 ggttcttcaa gagaccatcc aaaacgcact cgatcgcagt cgggtggttg cgttgatcgg
      601 tccacgccag tgtgggaaga caacgatggc gcgtctattt gtcccttcca cctcaatcaa
      661 ctattttgat ctggaagacc cattaagcct cactcgcttg gatcaaccca tgacggcgtt
      721 gcaggatcta cgcggcctgg ttgtgattga tgaagtccaa cgccgcccgg atctattccc
      781 aatactgcgg gtattggtag accgcactcc actgccggcg cgttttctga tactcggtag
      841 cgcttcacca gacctgctac gccagccgtc ggagtcgctg gctgggcgta tttcgaccat
      901 caccatgagc ggcttcagcc tggctgaagt gggtaaggag gcgcaaaatc agctatggag
      961 gcggggtggc tttccgctgt cattcacggc tgacaccgaa gaaactagcc ttgaatggcg
     1021 caaggatttt gtacgtactt ttcttgaacg ggatatcccg cagtttggtt tcaatatccc
     1081 ctccacgagc ttgtttcgtt tttggagtct gctggctcat taccatggac agatatggaa
     1141 taacgccgaa gcagcgcgca cattgaattt tggtgaagcc acagcccgcc gctatctcga
     1201 tctgcttcag gatttgttca tggtgcgctt gttacaaccc tggtatgcca atcttagtaa
     1261 gcgccaggta aagacaccca aaatctattt tcgagactct gggttgttgc accatttgct
     1321 gggtatccgc agtgagaagg atttgctctt gcacccgcgg agtggcgctt cctgggaagg
     1381 attcgctata gaggaaactg tcaaggcagt catgccggat gaggtttatt tttgggcgac
     1441 acattcgggg gcagaactgg atctgctgct catcaagaat gggcagcgca ttggcgcaga
     1501 gtataaacgt gtggatgcgc cacggctgac gccttcgatg cgcactgcct gcaaagattt
     1561 ggaacttgtc cggttgttgg tcatttaccc tggacttaag ccttatcctc tggatgagaa
     1621 tatccaggct atccccttat caagcctggc ggaaatgcca gtaaacttgt gttaacagaa
     1681 gcttccaata gtgctacaat ttatgcgtca actaatcgat ctctctctgt agcgaaatcc
     1741 attggatgtt tatctgttga cagaaactta aagacatcat ccaagagact tttctcgcca
     1801 ttagtccaat ggatcgggtt gaaccgggta ggcacttgga tgggagcagc ggtttttggg
     1861 aggtcggcca tttaagccat tggttgagag ttcgagcctc tccgcgctca ctgaaaataa
     1921 cttgcctttc ggcaggtttt tgcctttaat ccccccacaa taacctaaaa tcggaaattc
     1981 ttaattccaa tatacggcct tttttgtctc aaggtctgtc aacaccagtc aaccaattat
     2041 ttgaaaggaa ggtgtattat ggctaaaact aataagaagg atttgatggt tatatcgctt
     2101 gacaaagggg ttaaactgta tctgtcaact ttcgccatgg aggggaaaag cgcgcattgc
     2161 atcaattggc tgaatacaca acacgcggct aactgcgaac gacatcccac agatacatcc
     2221 gcgggggcag ttcaggatta gcccaagtcg ccatccaagc atatggggtc tctgcagagt
     2281 aggggggcgc cagatggttt ttcgctacct ggagtcccct ggaccagctt tttcgtcccg
     2341 gagaccggtg tcgcccttca tggcaaattc tggcacaaaa actttggggt gcaaatgagc
     2401 catgggcgcg tcaatatgcg caacgataat gccaagtggg tcttccggcg gtcggctgcg
     2461 gtttgggaga tcccggtaca ggatcggttt gcctgggaca ggcgaggcta tggtacctgc
     2521 gtggatgtcc actgacgcag ccgccatttg atgcttgtca ggacaattct tatccgatat
     2581 actctgttac gagacttttt ctcttgttcg gaactgctaa tggagtaggt ttgtattttc
     2641 ctcaatacct gcactccctt ccaagaagga cctccttgtc tctcaatatc aatagaataa
     2701 caaatgactg atattattct cgagacaaaa attaccccgc ccaaaaaacg atcaaaaata
     2761 ttgccgcgac cgcgtctgat tgagcgtctg gataagagct taaacaggaa tttgaccctc
     2821 gtgtctgctc cagcaggata tgggaagacc actctgattt cgagttggat taataatttg
     2881 caggaaccta tcgcctggta ctctttggat gcccaagaca atgacctttt tgtttttcta
     2941 agttatctta ttacagcctt gcaatcggct agtggggatt tcgaaaaacc gattgataaa
     3001 tttctagaaa ttgtttccca gccagatcga aaaccaaacc acaaaaaggt atttgctcaa
     3061 ttaatcaacg aaatcgcatc atttccccaa agagtggtcc tggtcttgga tgattaccat
     3121 gaaattaaag aaccttccat ccacgatttg ataagtgatc tcttaacaca acaacctcca
     3181 cagattcaca tcgttatcac aaccagaatc gatccgcccc tatcgattgc caggctgcgc
     3241 gtgagcgatg aaatagctga aattcgagcc aaagagctgc gcttcttggg agaggaaatc
     3301 agggattttc tcgatcaaat catgtctctg catttgccag agccacaaat cgccgctctg
     3361 gagtcgaaaa ctgaaggttg gattgctggt ctgcttctgg ctgcccattc cctgcaatac
     3421 gagccggagt catcgaagtt cgtagaagcg ttcgcaggga ccgatcggca gataacagat
     3481 tatcttattg aagaggttct ggcgcgttta ccccaggaaa ttcatgaatt ccttctttat
     3541 acctcggttc ttgaccgttt aactgcctct ctttgtcaac gattggtata tggtgaaggc
     3601 acagcggagc aaagccagga aatcttggag tggcttgaga gaaacaatct gtttacgttc
     3661 ccgttggata accgccgctt ctggtaccga tatcacttat tgtttgctga gtttttacgt
     3721 taccgattgg ctttagtttc tcccgagaaa gtattcgatc tccatctcaa agcaagtgaa
     3781 tggatggaag cgaatggatt tgttactgaa gctatcaacc atgcgctggt gggtgagaat
     3841 aataggcgag ccttagactt gatcgagaat cacggcttgg ccgcgatatc caaagcagaa
     3901 gtccgaaagg ttcaaagatg gttcgaaaac ctttccaagg agatgatacg atctcgttct
     3961 tccctttcta ttctttatgc atggaccctt ttgttcacca attattccca tcccccgaag
     4021 gaaattgacg aatggattca ggcggccgaa gaagcactgt cgatgcaaga aggtgaaccg
     4081 gataaagcca agaccgacaa agagcacaaa gtcagagagc atatctatgc aattcgcccc
     4141 atactggcct tctccaggca tgaagatcct caggcagtga ttgaatcggg aaagcgagcc
     4201 ctcgatttta ttgatgaaag caattcctgg ttgagaagca tcttactgca ttcaattggc
     4261 gcagctcaca tcatcatagg agacattgaa tccaccagac gctttgactt ggatgcgcaa
     4321 caccatgcga agattagcgg tatggactac atggggattg ggttatatta cgacatggtt
     4381 tttatcgcgc ttcgacaagg caggctgcgt gaggcggaat cggtttgtcg tgaatggctt
     4441 gattatgctc accgccaggg gaaaatggaa tccgccactg ctggatttct ttatgctttg
     4501 cttggaaaaa tcttggtaga acgtaatgac cttgattccg cccaaacggc gctggcgaaa
     4561 gggatggatt tgcttccctt gagcggggaa gatgaactcc gtgatttgtg ccttgtggat
     4621 ctatgcaggc tgtatcaagc ccggggtgaa tggtccagtt cggaaagggc cttatcggaa
     4681 aactccattg attctgctgg tgaacgatcg gcttggactg aatccttcct gtccgcctta
     4741 aaggctttgc tatggctcag ggaggctgaa tatacacaat ccaggcttcg cttagtcttc
     4801 caatggctgg aaaaatacgc tcctgatcta ggagcggaca ccgaatatcc aatactcttt
     4861 cctacatccg agagagacta tattgtgcaa atgattaccg ccagaattca tatcgcttat
     4921 gcgcgaacct tgccccctac ctatcgagag aaatccattc aatcggtgtt aagcttcctg
     4981 aatggtcaac accaaattgc ccaaaaacgg ggatggggtg aacgggtgat ggaacttgag
     5041 atcatcaggg cgctagcctt agaaacaatc ggtgaattgg atggagcctt cgatgccctg
     5101 tcgcgcgccc tcactattgg ggaatctgaa ggctatatcc gcatcttcgt agatgaaggg
     5161 catgtcatgg ctaagttgct gtacgaggcc gcctcccggg agatctaccc cgagtacgtt
     5221 ggcaagctgc tggccaccta tccaaaggag aagccaaaac taagcctggc aatgggagat
     5281 gaagacgagg agtttgatca agtcgagccg ttgagcgagc gcgagctcga ggttcttgac
     5341 ttgatcgcac aagggctttc aaacaaagaa atttctcaaa aactctatat ttccctaaac
     5401 actgtcaaag gacacacaag caatatttat ggaaagctgg ttgtaaagaa tagggctcag
     5461 gctgtgatcc gggccaggag gattggattg ttgcaggggg aataatataa atttggcata
     5521 ttttgaaaat tggaacgggt tgtcatttta tctgatgaca gcccgttttt tctttataag
     5581 cattgtgcgc taaaggatat tcgataaata gaacaatacc tcaataacac ttatgggtcg
     5641 tcacgaacaa ccatctttta tgtatgctgt agataattat ccaaggagtg cagatatcgg
     5701 tgagaattgg gagctaaacc tcaatatccc atgaaaacat aatcgtatat tgcacttatc
     5761 tgaggaagga aaagaaaacc catagtcagt tcagcacaaa acaatttcaa cagagacaaa
     5821 agctatgtta ccccaattag aaattgaagc ctacaaagat agagtaaatg cctttttgca
     5881 agaggcagag aaacagcgga tgctgaaact gatccgtcta aggcgatcca aaaagagtca
     5941 aatcaagagc cctatacaga ccatgaggct gctatggata aacaaaggat atatcagatc
     6001 agagtaaaag gccagcttgg agagaaacaa tgggattggc ttgaggatat ggagacaagc
     6061 gtatcccagg atgaaggttt cggcaccatt actgcgatca cggggttggt cgaagaccag
     6121 gcgctcctcc atggaatcct gtaccgactc tatagtgtgg gttatgtgct gatttccgtg
     6181 aactgtttgg atgagggcgg acgcaagtct taaggtgtga atatcaggat gcctgtgacc
     6241 aaatgcacga caagtgaacg gatgtatgga tatggacgaa gaaaacccgt tgattacact
     6301 acatgaggac tgggctaccg ccatcgaccg tgttgagcaa gcccttgctt ctgcgggctt
     6361 tgaagttatc ccatctttcg acttacaggt cgctcaggca gaagctggga actttatctg
     6421 ccccatccac agcaatggac gatgcgatgg ccagatgatt gagctcctgg tttatgaccg
     6481 ggataatccg ccgctcaccc tggaagttct tggtcataat ggcctggtac gttttgcgat
     6541 agtagatgcg cctcaaaggc aatccaaccc ccttctgcga gcagtgatct tacaggcatt
     6601 aagggaaatg agtatttcaa agcaataccc cggagttggg aaccatgacg gataaccgga
     6661 agagaattct ttggcgcctg cgtaccgtcc agggacatct gcgtgcgatt ggcgacctct
     6721 tcgaagccgg ggaaacctgc gaggagatac tcattcagtt gaaagctgta cgaggcgcca
     6781 tcgatgcgat cgcagcccaa ttgttttatg accaagtcga aagaagcaag caagtgattt
     6841 gtgaggagcc ctctctcaac cggcgctcag aagaattgga acgcttagtg aagctgtatt
     6901 ctgcatggaa acaatcgagc tttattaact acaacaccgt ctcaaaagga gcatgcagca
     6961 gagccgccgc attccccacc aaactgcaat tttttgaaga tatctaatca aaacataagg
     7021 agattaaatc atggaccaat caatcgagtt tacaatgccc gtttccggta tgacctgcgc
     7081 ctcatgcgca gccaatattg aaaacgcatt gaatggcgta gagggggtgg cgcaggcaag
     7141 cgttaacctg gctaccgagc gcgccagtct tcgcctgtca aagcctgagg tcaagacgat
     7201 cgacttgatc caggctgtaa gagatgtcgg ttacgacgta cccagtgaga ccctcacttt
     7261 gcccataggc ggcatgacct gtgcctcctg tgtatccact gtggaaggcg ctctcaaggg
     7321 tgttcccggg gtagttgatg ctaccgtcaa cctcgctaca gaaaaagcta cagtaacttt
     7381 cgtccccacc ctggcaacga tcaaggattt caaagttgcg gtggctgaag ttggatatca
     7441 agtcctggac ttcaaggaag gcgccggtca agtcgccgaa gacgagtctg agcgcaaaat
     7501 gcaggaatct cgcttcagga tgtgggtcgc ctggagtttc accgcaccca tcattttatg
     7561 gatgtttctg gagatgttct ttggaatcat gtggcccaat gagtccatat ttaatctagg
     7621 gatggtggta ctgggtctcc cggtcttgtt ctgggttggc agacatacct atcgttcagg
     7681 atttaaggct ggaatacggg gcaaaccaaa catggatacg ctcattgcgc taggcacggg
     7741 cgtctccctt ctcaccggtc cagcttcctt cttcttcccc gtcgccaact atgcgggtgt
     7801 ggcagccatg atcatggcat ttcacctgac cggtcgctat gtggaggaaa ccgccaaggg
     7861 acgcgcttct caggccatcc gcaagctcct ggaattggga gcgaaaaccg cttgggttct
     7921 taaagacgga aaggaagttg aagtccctat cgaggatgtt gacgtcgggg acatcatgat
     7981 cgttcgccct ggggacaaaa tccccaccga tggagtagtc ttacaaggcg atagtgcggt
     8041 tgatgagtcc atggctactg gagagtccat gcctgtaacc aaacgccagg gggatgaagt
     8101 aattggcgct accattaacc aggaagggct gctcaaggta aaagccactc gagttggcaa
     8161 agacaccttc ctctcccagg ttatcaagct ggtggaagaa tgccagggtt ccaaagtccc
     8221 gatccaagaa tttgccgata aagtaaccgg catcttcgta ccgatcgtgc tcgtaattgc
     8281 gatcctgacc ttcgctgcct ggatcttaat ttctgcaccc atgcaagctt tggttaatac
     8341 tggatccttc ttgccctggg tcaatcccaa tctgggggtg atcaccctgg ccattgtctc
     8401 caccgtagct gtgctggtta ttgcctgccc ctgcgctctc gggttggcca cccctaccgc
     8461 cctgatggtg ggtagcggca agggcgccga gcacggcatc ctcattcgca ccggtgaagc
     8521 ggtccagacc atcaaggacg tgcgcatcat tgcctttgac aagaccggta ccatcaccaa
     8581 gggacgtccg gaggtcacgg atatccttcc agccgaaggt tttcatcaag atgaggtcct
     8641 ccgtctggct gcttcggtcg aggttgggag tgagcatccc ctggggagat gcatagttga
     8701 gaaatcccga gtccttgagc ttattctaga agaaccacag gcctttttgg ccatacgtgg
     8761 gaaaggcgta agtgctacgc tcgatagtgg ctctgttctt gtgggatcac gaagactgat
     8821 gctcgagcac ggaatcgatc cagcaccttt agagaaaacc ctgcaacagc tggagaatga
     8881 agccaagacc gctatgctcg ttgctgccgg gggcaggctc gccggggtaa ttgccgtggc
     8941 agacacgctc aaggaagact ctattgatgc aattcgagag ttgcatcaca tggggcttga
     9001 gaccgccatg atcactggag acaatcgacg tactgccgaa gcaattgcac gcagggtcgg
     9061 cattgatcac gtgctagctg aggttctgcc tgatggaaaa gtggcggagg ttcaaaagct
     9121 gcaggatcgt tttggtttgg tggcgtttgt aggtgatggc atcaatgacg cccccgccct
     9181 gactcaagcc aatgtcggca tcgccatcgg aacaggaacc gacatcgcca tcgaagccag
     9241 tgatatcacc ctggtccgag gtgagctaac cggcatcgta gaggctatca acctcagtaa
     9301 tgctaccttc caaaagatca agcaaaacct gttttgggca ttcttctaca acgtggccat
     9361 gatcccgctg gcaatcatcg gctggatgca cccggtgcta gcggagatcg ctatggcgac
     9421 gagttccatc accgtggtca gcaacgccaa tcgtttgcgc agcgtcgaca tccgccccag
     9481 ctacttgcgt ggtgagaaag tttccaaacg agttcaggag tcttcgtatt cctcaaagga
     9541 aatggtcgtc aaggcataag attccaggat atgaccgcaa cccgatgagc gcctttgctg
     9601 aaatatgtga gcattaatat gtcaaccatg agacgatttc gagcgccgtt gcttcccatc
     9661 gactccccca ccgggagaga gcgggcattg atcgtgattg agcgcctgat caaaggagcg
     9721 ctcttcaagt gcagtatgtg tggtaactgt ctgctgcaag agacggcttt catttgcccg
     9781 atgctgtgcc caaaagggtt gcgtaatggt ccctgcggca gtggcgctgc tgatggttgc
     9841 tgtgtagatc catcccgccc atgtgtctgg cacttgatct accagcgcgc tgaagccaaa
     9901 ggctctctgg ataagctctt ggaagtccag gctcccctgg actggacgag agtgggccat
     9961 gagacctggg gcacagtgct tgccgaggcg cgctcgcgtg ggttgttttc tcccagtttt
    10021 ctggcccacg ccgacgaatg gcgaggagaa gtgacggaac tctttcgcgg aatacgccaa
    10081 ccagaatggt ggcagggtga tgaccaatat cacccgcctc gttcaaatgg cgctgcatca
    10141 aggctgcagg cggctctgga taatggcgag ttcgttctta ccgccgaggt cacgccgcct
    10201 gcagactcaa atacagagga agttctccaa gcagcggttg gctttagaga tgttgttcat
    10261 gccgcaaacg tgacccagaa ccctatggca accacgcgca tgtctagcct ggcctgcagt
    10321 gcattgctca tggaagcggg tgtggaacca gtgcttcagc taacagccag agatcacaat
    10381 cgctttaccc tgcaatctga ggcgctcggc gccacagcgt tcgggattag caatatcctg
    10441 tgcctgacgg gcgatcctcc caccaaaagc cgggggccag ctgctggcct gccattcgac
    10501 cttgacgcca cccaaatgtt gtggatcctg cggcgaatga gagatgaagc ccaactcctg
    10561 gacagtcgac cccttaagaa accaccgatc ctattcttgg ggacagccgc ctcgcccaat
    10621 gaccctatac cccagcacga agccaggcgt ctggaaaaga aaatcaatgc cggagctcaa
    10681 tttatccaga ctcagcttgt ctatgacacc gccaggttga aaagatggtt agccgcccta
    10741 gcagaacgag acttgctcca aaaggtgcat atcctggttg gtgtaggccc actgcgttcg
    10801 gttcgcatgg cccggtacat gcgtgaacac attccggaca tctccattcc gccagggctc
    10861 gtcgatcgaa tggaacgcag ccctgacccc aaggagactg ggctggaaat cgcattggag
    10921 ttagttgacc agattaagga tttgcccggc gtaagtggca tccacctcat gagccttggt
    10981 tggaaatcga tcctgccgcg ccttctgaag gaagcaaggc tttgaatcta agcctgggag
    11041 ccagatctag gaccgcagca tctgagcaca ttgaacacat ccatgatcag tacaagcctg
    11101 gcagcgacca acggcaagtc tgacccgtcg aaacgggaag agttgaatga tgaagtttac
    11161 tgtatcggag atcttccaac agagccgacc acctgatgcg acctcaccgg cgcgatcaac
    11221 caagcgcctt agcctggcga ggctggcacc aagccgggga ttatctcttt tactgttttc
    11281 ttctgccatc tctgaagtgg catgcaatat tgtctacatc gtccttcttg agcgggcata
    11341 tttgctcgga aggggatctt ccagcatagg cattctcatg atcatccagt cgaccgcccc
    11401 ggttttcttc ggttcctgga tgggcggact tgcagatcag ttgagattta agcgatcggc
    11461 agtcatcgca aactcagcca ttgctgctct ggttttcagc ctgactttcg caaagatcgt
    11521 tacgttcgtc tatatcattg cgttcctgat catgttaggc cgcctgctgc ttataccggc
    11581 ccgattgggc ttggttgccc aattgaccga tagagctcgg ctcatggaag ccaacacaac
    11641 cctttcgatt ctgtctggcg tgggaagctt tataggacct gccatcgcag ggatgctgtt
    11701 tctggcctcc gacgacttcg ctccagcgct tttgggagcc ggcggcgggt ggctgctctc
    11761 attgctaccg ctgaccatga tccagatgaa accaggaaat ccgatcgccc ttcggcgaac
    11821 gtttcccatt gaggaaatac gaaatggttg gcatctgatt cgcaaacgga gtacgatcac
    11881 aaaggtttta tggtgcctga tcgttgcaac gctgctcttg ggcgcagtca caccgctcat
    11941 tacgcctctt tctcgacaat tcggcctggg ttcagaaggt acaggcgttt ttttcagcgc
    12001 tttggggttt ggcacactga tcggtccgct catcgccact ggtctcttca aacggttgca
    12061 cctatccacc accttgttga ttgtagggct gttggcgccg gttgggctta ttgtcgtcgg
    12121 aatgttaaac cacctgcagg gtgtactcat cgcgatcgcg gtcgcagcga cagcaggcac
    12181 cggtctgaac gtaatcgtga caacagttac ccagcgtttg tcccggtcaa gccaccatgg
    12241 gacggtattt ggcacccagc aaaccctgtt gggtatcgcc tggatattgg ctctggctgc
    12301 caccactggc ctgacggcgc tttgggaact tgagtcgaaa gttcggatgc tgttcatcgc
    12361 acttggcggt tccggtttcc tggctatcct tagctgctgg acctggaatc gacaatccat
    12421 acggaaaacc tgcgaccttt gcgaaccgga actccgcatt gcttcagtcg cttgtcggat
    12481 tgttcatggt cccccatatc agatatcggc tactttgtgc ggcatcattt gcggtgaagg
    12541 atgcgggcgt tgctattccc agtaaaaccc gaccaatgag gaacaagaca atgagcgatt
    12601 ataaaaatga taaaggactc tcttttttgc aagaaataat caactataca cgcgattggc
    12661 gtgcaattga gcttggtgtc catatctata gacccagcaa taatatcttc atccggggtt
    12721 tgattagcgc agggcgtatc gctagaggct gccgtaaaaa taaggtgaaa cagggtttgc
    12781 ggcggcgcag cgcccgtaag agcctggaat ctgccatcat caagacgagg tccacgatct
    12841 atgggagtag aacctgaatg atatggttta aggaggtgag actcatggtg aacaagaaat
    12901 gttggatgta cactaggttc gctcactgtt ccaaaggcta ctgttgccag tcctgggcaa
    12961 gactttaaac tgctcggtcc tgggtacgac ctaaaactgc ccgccaacta accttaacac
    13021 agcttttaat atattcttcg gaaggaggtg agacaaaatg gtgagatgtc cagtctgtgg
    13081 catgatggtt aatgaaaata ccgcccccag ttctgagtac aagggcaaaa aatattattt
    13141 catgaacccc acacacaaag cactgttcga taaagaccca gaaaaattct tgagtcgaca
    13201 attgaaccga agcgaccatt aagggaaccg ctacagccga attttggagt agcgtatcat
    13261 tccttctgta atttctaacc gcccaaaaaa gcggtaggtc tggtgtatcc ttcaagttgc
    13321 cacacaagaa aggagcacac catgacctac cagggcggtt ctaccttacc acccgac
//
LOCUS       DBNGSX010000371         4409 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_934126, whole genome shotgun sequence.
ACCESSION   DBNGSX010000371 DBNGSX010000000
VERSION     DBNGSX010000371.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_934126"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..1024)
                     /locus_tag="AC16CB_11170"
     CDS             complement(5..1024)
                     /locus_tag="AC16CB_11170"
                     /inference="COORDINATES: protein motif:HMM:NF014841.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HIR3343073.1"
                     /translation="MNDTIEVLIALPFPDKLIERLIEISPRLKFTVKSPSKPDDISPD
                     LWAHTEVLYTARVLPTAELAPNLRWIQFHWAGVDHAIDDPILRKPDLVATTLSGAAIS
                     QMAEYAVMMLLALGRHMPELVTLQNRSEWPHDRWERFTPIELRGSTVGIIGYGSIGRQ
                     LARLLSYFGATVLATKRDAKHPQDSGYIPEGMGDQGGDYVKRLYPAQALRTMLKACDF
                     VVVCVPKTPGTINLIKAEELAAMKPSAYLVDVSRGGIVDQQALLAALKDHKIAGAALD
                     VFPEEPLPAKSPLWKLPNVLITPHISGITSHYDDRAVELFSANLLRYLAGQPLYNRID
                     PKLGY"
     gene            complement(1216..1461)
                     /locus_tag="AC16CB_11175"
     CDS             complement(1216..1461)
                     /locus_tag="AC16CB_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343074.1"
                     /translation="MSIEHFHIQNYAYCKSRHSELINETKTARQVRLIMESQRTQGRA
                     FTRVLRWLGRCLITWGEMLEERTGSRDEIPVPSARFT"
     gene            complement(1463..3010)
                     /gene="pdxR"
                     /locus_tag="AC16CB_11180"
     CDS             complement(1463..3010)
                     /gene="pdxR"
                     /locus_tag="AC16CB_11180"
                     /inference="COORDINATES: protein motif:HMM:NF052282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070279 - vitamin B6 binding [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal 5'-phosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:2000142 - regulation of DNA-templated
                     transcription initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MocR-like pyridoxine biosynthesis transcription
                     factor PdxR"
                     /protein_id="HIR3343075.1"
                     /translation="MRIPIDRESATPIYKQIIQFLRDEIESGALLAETRLPSTRSLAE
                     SLGVNRITVTNAYAELEAEGLIYGKTGSGFYVAELMLPQMRMNTGPATSTDWPLWQQS
                     LLSRGWTPVMGESERLAHTVESPDLISFSQGISDFRLIPADDIRKAIQAVLRRDGVDA
                     MGYGDRTGYPPLRETISHILASQGVPTHPDSILITSGSQQAVALVAHLLLRPGDVVLV
                     ESPSYIGVIDLFRSMDVRLLGIPIDEQGMRMDALEDALRNVNPKLIYTIPTFNNPTGV
                     CLSSQRRRQMIALADRYNTPILEDDFAGDLRYEGRAQPALKALDPGGLVIYVSTFSKM
                     LAPGLRLGFLAADGPVYERLVACKQAYDLATSNLIQRALEAYITVGRYQAHLRRACQV
                     YGSRRDAMISALSKYLPAEASWNKPQGGLFVWLKLPDELSTNELYPLAGEEKVSFAPG
                     SLFYPGARHQPYMRLNFVAHPVDVIEEGVSRLGRAVERLIAQKQGGRQNNKIPIQSDR
                     KIQNLEV"
     gene            complement(3192..>4409)
                     /locus_tag="AC16CB_11185"
     CDS             complement(3192..>4409)
                     /locus_tag="AC16CB_11185"
                     /inference="COORDINATES: protein motif:HMM:NF025011.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HIR3343076.1"
                     /translation="LTAPLGRPATPSERAHTILANDRILDHACTLLAALRLGLHLDSV
                     EFTRASWAFTSPFAPSPTALLAMLNATDLLDAGTNLPQPEATRQFLEYPRGKAIALLA
                     RAWQHSPAFNEFRMLPDLSFEGEWQNDPLRARYAIIDLLSKVPHNTWWSLPGFVSAVK
                     HHQPDFQRPAGDYDSWFVRHKVSGEYLRGFEHWDEVDGALVRYIICGPLHWLGVLDLA
                     APAPGAQPAAFRFSRWSVELLSGAPAQGLEVEDDSFLISSDARLRAPRLVPRAARYLV
                     TRFCAWEGANEDGYLYRITSDSLSRARDQGLRAGHLLSLLRRHALTVPPSLTRALERW
                     EEHGSEARLERALILRLRTPETLQLLRNSRYARYLGDPLGPTTIIVNPGAGEKVLAAL
                     AEMGYLGEMEGRE"
BASE COUNT          964 a         1161 c         1265 g         1019 t
ORIGIN      
        1 caagtcaata tcctagcttg ggatcgatgc ggttatataa tggctgtcct gccagatagc
       61 gcagcaggtt ggcgctgaac agttctacgg cgcgatcatc gtaatgggaa gtgatgccgg
      121 agatatgggg tgtgatcagg acgttgggca atttccagag cgggcttttg gctggcagag
      181 gctcttcagg gaagacatcc agggctgccc ccgcgatttt gtggtctttg agggcggcga
      241 gcagggcttg ttgatctaca attccacctc gggagacgtc caccaggtag gcgctaggtt
      301 tcatcgcggc caattcttca gccttgatca ggttgattgt gccaggcgtt ttgggcacgc
      361 ataccaccac aaagtcacat gctttgagca tcgtgcgcag cgcttgggct gggtagagac
      421 gcttgacata gtcaccgcct tgatcgccca tgccttcggg gatatatccg gaatcttgtg
      481 gatgcttcgc atcgcgtttg gttgcaagaa ccgtagcgcc gaaatatgac aacaggcgcg
      541 ctagctgtct cccaatgctc ccgtaaccaa taatgccaac cgtgctaccg cgcaattcta
      601 tgggtgtaaa acgctcccag cggtcgtgcg gccattcgct acggttttga agggttacta
      661 actcgggcat gtgacgccca agcgccagta acatcattac ggcgtactca gccatttgag
      721 aaattgctgc accgctcagt gtcgttgcca ccaggtccgg cttgcgcagg atgggatcgt
      781 cgatagcgtg gtcgacgcct gcccagtgga attgaatcca gcgcaggtta ggtgcaagct
      841 ctgcagtggg aagaacacgg gcggtatata gcacctcagt gtgtgcccac aaatccgggg
      901 agatatcatc aggcttgctt gggcttttta cggtgaattt caggcgcggt gagatttcga
      961 taagccgttc gattagttta tctgggaacg gcaaggcaat caggacttca atcgtatcat
     1021 tcatggttag ccattatagc aaaaattgaa gcacgtggga gcgcgacgca tacctcaaat
     1081 tttattctat taggacttac gcagttaata aggaaaggag gggggtttgg gcggacatcg
     1141 cccgcccaaa cccccctgcc acgtatccaa ctgcgtaact tctatctata tttctaacac
     1201 tgattgattg tgaaatcagg tgaagcgagc ggatggcaca gggatttcgt cccttgagcc
     1261 agtgcgttcc tcaagcatct ctccccaggt aatcagacat cgtcccaacc agcgcagcac
     1321 tcgggtgaag gcacggcctt gtgttctttg actttccatt atcagacgta cctggcgcgc
     1381 tgtctttgtt tcattgatca gttcggagtg cctgctttta caatacgcgt aattttggat
     1441 gtgaaaatgt tcgatagaca tatcatacct ccagattctg aatctttctg tctgattgta
     1501 tgggtatctt gttattttgc ctgccgccct gcttttgggc tatcaagcgt tccacagccc
     1561 ggcccaggcg gctcacccct tcttcgatta catctaccgg atgggcgaca aagtttaatc
     1621 gcatataagg ctggtggcgc gccccgggat agaagaggga tccgggcgca aatgagacct
     1681 tttcttcccc ggctagcgga tacagctcgt tggtggacag ttcgtcgggg agtttgagcc
     1741 acacgaacaa gccgccttgt ggcttgttcc agcttgcctc tgcaggcaga tacttgctta
     1801 gggccgatat catagcgtcc cgccgacttc cgtaaacctg gcaggcgcgg cgcaagtggg
     1861 cctgatagcg gccgacggtg atgtaagctt ccaaagcgcg ctggatcagg ttggatgtcg
     1921 ctaggtcata ggcttgcttg caggctacca gtcgttcgta aaccgggcca tcggctgcca
     1981 ggaaacccag ccgcagacct ggtgcgagca tcttggagaa cgtgctgaca tagatgacca
     2041 gcccgcctgg gtccagcgct ttgagcgctg gctgggctcg gccttcgtag cgcaggtcac
     2101 ccgcaaaatc gtcttccagg atgggtgtgt tgtagcgatc agccagggcg atcatctgcc
     2161 tgcgccgctg gctgctcagg cagactccgg tcgggttatt aaaagtggga atggtataaa
     2221 tcagcttggg attcacattg cgcagggcat cttctaacgc atccatgcgc atgccttgtt
     2281 cgtcgatagg gatgcccagc aaacggacat ccatcgagcg gaacaggtcg atcacgccga
     2341 tataggatgg gctttcgact aaaaccacat ctcctggacg cagtagcagg tgagcgacaa
     2401 gtgccaccgc ttgttgagag cctgaggtga tgaggatgct atctgggtgg gtgggcacac
     2461 cttggctggc caaaatgtgt gagatggttt cgcgcagggg agggtaacca gtgcgatcac
     2521 cgtaccccat agcgtctacg ccatcccggc gcaatacggc ttggatcgct ttgcgaatat
     2581 cgtcggctgg aatcagccgg aagtcgctga tcccctgcga gaaggagatc aggtctgggc
     2641 tttcaacggt gtgggcaagt ctttcgctct cacccatgac gggtgtccag cctcggctca
     2701 acagggactg ttgccataaa ggccaatctg tacttgttgc aggacctgtg ttcatgcgca
     2761 tttgtggcag cataagctca gcgacgtaaa aaccgcttcc tgttttaccg tagattagcc
     2821 cctcggcctc caattcggcg taagcgttgg tcacagtgat gcgatttacg cccaagctct
     2881 ctgccaggct gcgggtggag ggcaggcgcg tctcggctag cagtgccccg gactcgattt
     2941 catctcgtaa aaactggatg atctgcttat atatgggcgt agcgctttca cgatcgatag
     3001 gaatacgcat acttagctcc tttaatctat tcgatccaat tggtcgtctg gtatatgtat
     3061 actacatcga aattgggaaa ttatatagat ccaattggtg ccaatctata ccaggccgcg
     3121 tgaattatcc cccatatcta aacctgacta cattgtctta aatagggccg attacccccc
     3181 taattctcaa cctactcccg accttccatc tcccctaaat agcccatctc agccagggcg
     3241 gcaagcactt tctctccagc cccaggattg acaattattg ttgttggccc cagcggatcc
     3301 cccaggtaac gggcatagcg cgaatttctg agtaattgga gagtctccgg agtccgcagc
     3361 cgcaggatga gagcgcgctc caggcgcgct tcgctgccat gttcctccca gcgctcgagg
     3421 gctcttgtca ggctaggagg cacggtcaag gcatggcggc gaagcagact gagcaagtgc
     3481 cctgcacgca aaccctggtc gcgagcgcgg gatagagaat cggaagtgat gcggtacaaa
     3541 tatccatctt cgttagcgcc ttcccaggca cagaagcggg tcaccaggta tcgagccgct
     3601 cgaggcacaa ggcgaggagc gcgcaggcgg gcatccgagc tgatcaagaa agaatcgtct
     3661 tctacttcca agccttgagc gggcgctccg ctgagcagtt ctacagacca gcgcgagaag
     3721 cggaatgcag ctggctgtgc tccgggggct ggcgcggcca gatctagaac gcctaaccag
     3781 tgcaatggtc cgcagatgat ataacggact agcgcgccat ccacttcatc ccagtgctca
     3841 aaaccgcgca gatattcacc gctgactttg tggcgaacaa accatgagtc gtaatctccg
     3901 gcagggcgtt ggaaatcagg ctggtggtgc ttgaccgcag aaacgaagcc aggcaagctc
     3961 caccatgtgt tgtgaggcac cttagagagt aagtcaatga ttgcatagcg ggcgcgcaag
     4021 gggtcatttt gccattcccc ctcgaaactt aagtcaggta acatgcggaa ctcgttgaaa
     4081 gccgggctgt gttgccaggc gcgcgccagc agggctatcg ctttccctcg cgggtattct
     4141 aagaactggc gggtcgcctc tggttgaggc aagttggtac cagcatccag caagtccgtt
     4201 gcgtttagca tggcgagcag ggcggttgga ctgggggcaa atggtgaagt gaatgcccag
     4261 gatgcccggg tgaactcgac tgagtccaga tgcaatccaa gacgcaaggc tgccaatagc
     4321 gtgcaagcat gatccagtat gcggtcgtta gcgaggatcg tgtgagcgcg ctcggatggc
     4381 gtggctggtc ttcccagtgg agcggtcaa
//
LOCUS       DBNGSX010000372         5912 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1140654, whole genome shotgun sequence.
ACCESSION   DBNGSX010000372 DBNGSX010000000
VERSION     DBNGSX010000372.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5912)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1140654"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1719
                     /locus_tag="AC16CB_11190"
     CDS             <1..1719
                     /locus_tag="AC16CB_11190"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HIR3343077.1"
                     /translation="PTRLEIPLRAGQERLGGLLVEVEQARGVSEDEHAILVSAANLAV
                     LAIVNARLFQEQRLQRLDAEERENERRERERSLTLFGEITQAALGSEDYPAMLQALAD
                     NLATAFEADGVLILLLEEGTGRLLGEAAFGPLRPTARTLQYDPQEMILGKWVLDTGEA
                     VAIQDAVVSPYIDQRVATEMQSRSILALPLVADKEKLGIAIISYYSEHRFSPQEINAG
                     EQAARQIALAIAKGRALAMAQYRAQELDALQKATAALLSTLDLEQLLGQILDAAISAV
                     PSAHRGSLHLIAHDTGQLQVRAIQGYTDSRIRAINRTGGSHIARAVRERRSLMVEGPA
                     AEVFVAPGEAGSGSREAVSSIIAPLLLGDETLGAISLESYRPYAFIQPDLDLLVSFAA
                     TATTAIQNAQLHAEVQRQAITDTLTGLYNRRGFFELGRREVERAIRFGRPLTAVMFDI
                     DYFKNVNDLYGHAVGDRLLINLTTRLLQELRQIDLLGRYGGDEFVALLPETDLSNAMS
                     VAERLRKVVASTKVTGPSASVNISLSAGVATLSNDCRDLNSLLQQADKALYEAKRQGR
                     DRVVSM"
     gene            complement(1721..2362)
                     /locus_tag="AC16CB_11195"
     CDS             complement(1721..2362)
                     /locus_tag="AC16CB_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343078.1"
                     /translation="MPASLEDHLKKDQIALFRSLDHPAKIQAFLDEIPYSAEDSNRSP
                     LKVLQDHQAHCLDGGLFAAAALRRLGFPPLVVDMYPDPGMDDDHVLAIFVVDKHYGAI
                     AKSNFVGLRYREPVYRGLRELVMSYFADFYNVDGVKTLRTYTRPLNLKSFDRQGWEWS
                     DDGVDAIEKRLLGLARHPLLKPEVAAGLSPMDPLSYKAGMLNVNYAGLYKPEK"
     gene            2547..3431
                     /locus_tag="AC16CB_11200"
     CDS             2547..3431
                     /locus_tag="AC16CB_11200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3343079.1"
                     /translation="MNATVEMTRAQPSITLSDVETTGLLTLYCHALESQSTNPILKDD
                     KAVEIARQLTPLLIDSKDKLLRKLATNKVDQRLVVHIALRAKQYDQYAKNFLDQNPDG
                     IIVNIGCGLDPRFFRIDNGSMIFFDIDLPEVIRFKKKILAESDRYRMIAASVFDYQWM
                     DEVAQYGSRPVMFLAEGVFMYLEAEKVKSLVLELQSRFPGSELVCEVVNEMWVRQPLN
                     YLVSIKMQRQLSLGKDAVYRFGIASSRQMETWHAGIQYLDDWSYFDTDHEKLGWMKWI
                     GKSEFMRKVQWTVHYRLN"
     gene            3461..4090
                     /locus_tag="AC16CB_11205"
     CDS             3461..4090
                     /locus_tag="AC16CB_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3343080.1"
                     /translation="MYENFERIPPDEQARILESCLEEFALHGYEKASTNAMVKRANIP
                     KGTLFFYFGSKKDLYLYLIDRAVERFVAFMSQANDELPSDLFERLLHRSKIRFQFALQ
                     EPTLYQFFYHALINTPESIQSDLQSRYSTYALASRQSLYEGLDRSKFKDGIDIEQAIE
                     LVYLVLEGILSRYTGLLKKIQPDEGLQLVEKLTDECWGYFEILKQGMYK"
     gene            complement(4212..4451)
                     /locus_tag="AC16CB_11210"
     CDS             complement(4212..4451)
                     /locus_tag="AC16CB_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3343081.1"
                     /translation="MAPSLRFYTLLGLDIPANMDAEQHVEITLPNGFRIAWDSLEMVK
                     SIYPDWVDPVVGQRMTLAFRCASPAAVDDLYNRVI"
     gene            4681..5499
                     /locus_tag="AC16CB_11215"
     CDS             4681..5499
                     /locus_tag="AC16CB_11215"
                     /EC_number="2.7.7.-"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3343082.1"
                     /translation="MTTEKTEHDTFVETAWRNLLIQGESNEEKRLRRIFRMFPAKTKC
                     KWCDLPFDHPASSLIHLVFRKRPSAFNPRFCNVCDDFAVKFQGGAEVELSMVFADIRG
                     STTLAEGMSATEFKKLIDRFYQVSTKIFIQTNAMIDKLVGDEVTAFYLPGLAGKDYAH
                     LAIEAAQELLIKTGHEDPYGPWAPIGIGVHTGVAFVGAVGTSDGMVDITALGDAVNIA
                     ARLASQAKAGEILLSEKTIQAAKLDSSNMEKRSLELKGKSSTFDVKVMRITPGD"
BASE COUNT         1448 a         1440 c         1560 g         1464 t
ORIGIN      
        1 cctacccggt tggagattcc gctgcgggct gggcaagagc ggctaggtgg attgctcgtt
       61 gaggtcgaac aggcgcgcgg ggtcagcgaa gatgaacacg caattctggt ttctgcggca
      121 aatctggctg ttttagccat agtaaatgct cgcttgtttc aggaacagcg cctgcagcgc
      181 ctggatgccg aagaacgcga gaacgagcgg cgtgaacgtg agcgctcctt gacgctgttc
      241 ggcgagatta cccaagctgc tttgggctca gaggattacc cggctatgct ccaggctctg
      301 gccgacaacc tggcaactgc tttcgaagcc gatggtgtct tgatcttgct ccttgaagag
      361 ggtactgggc gtttgctcgg cgaggcagct tttggcccat tgcgacctac tgcccggacg
      421 ttgcaatatg atccgcagga gatgattttg ggtaaatggg tgctagacac cggggaggca
      481 gtggcaattc aggacgcggt tgtctcccca tatattgacc agcgcgtcgc taccgagatg
      541 caaagccgat ctatcctggc tttacccctg gtggcggata aagaaaaatt gggcatagcg
      601 atcatatctt attattctga gcatcgtttc tccccacagg agatcaatgc aggtgaacaa
      661 gctgcccgtc agatagccct ggcaatagcc aaaggacgtg ccttggctat ggcacaatac
      721 cgcgcccaag agcttgacgc cctgcaaaag gccacggctg ctttgctctc cacgctggat
      781 ttggagcagt tgttgggaca aatactagac gcggccatca gcgcggtgcc ctcggctcat
      841 cgaggctcac tccacctgat cgcccacgat acaggtcaat tgcaggtgcg tgcaattcag
      901 ggttataccg attcccgcat ccgcgccatc aatcgtacag gtggctctca cattgccagg
      961 gcggtgcgtg aacgccgttc attgatggtg gaaggacctg cggcggaggt ctttgtggcg
     1021 cccggtgagg ctggctcagg atcgcgcgag gctgtctcat cgatcattgc ccccctgctg
     1081 cttggggacg agaccctggg agcaatatcc ctggagtcct accgccccta tgccttcatc
     1141 caaccggact tagatttgct ggtgagcttc gctgctaccg ctaccaccgc cattcaaaat
     1201 gctcaattgc atgctgaggt gcagaggcag gccattaccg ataccctaac gggcctgtat
     1261 aaccggcgtg gcttcttcga gttaggtcgc cgtgaagtcg agcgtgccat acgctttggg
     1321 aggccactca ccgccgtcat gtttgacatc gattatttca aaaatgtaaa tgacctgtat
     1381 gggcacgcgg tgggtgatag gttgttaatt aaccttacga cgcgcttatt gcaagagcta
     1441 cgtcaaatcg atctgttggg gcgctacggg ggagacgagt ttgttgctct gctgcccgag
     1501 accgacttga gcaatgccat gagtgtggct gagcgcctgc gtaaagttgt ggcttctaca
     1561 aaggtgaccg gaccgtccgc atccgtgaat atatcactta gtgcaggggt ggcaaccctg
     1621 agcaacgact gcagggacct gaacagtctg ttgcagcagg cggataaggc tttgtatgag
     1681 gcgaagcggc agggtcgaga ccgggtagtg agtatgtaga ctacttttcc ggtttgtaca
     1741 gcccggcgta attcacattc agcattccag ctttatatga gagagggtcc attggcgaaa
     1801 ggccggcggc gacttcgggt ttcaacaagg ggtgacgagc caatccgagc aaccgctttt
     1861 cgatggcgtc tacaccgtcg tcactccatt cccatccctg tcggtcgaat gattttaagt
     1921 tcaggggacg ggtgtaagtt cgcaaagtct tgactccatc tacattgtaa aaatcagcaa
     1981 aatatgacat gaccagctct cgcaagccgc gatagaccgg ctcgcggtac cgcagcccaa
     2041 cgaaattcga tttagctatt gcgccatagt gtttatcaac cacaaatata gccagcacgt
     2101 gatcgtcgtc catgccggga tctgggtaca tatctaccac gagaggtgga aaacccaggc
     2161 ggcgcagcgc tgctgcagca aataagcccc cgtccaggca gtgagcctgg tggtcctgaa
     2221 gcacctttag tggagatcgg ttgctatctt cggcgctgta gggaatctca tccaggaaag
     2281 cctgaatttt ggctgggtgg tctagagagc gaaatagggc gatttgatct ttcttcaggt
     2341 ggtcttctaa actggctggc atatgtatgg ttatctccta ggcttggata aaatttgcct
     2401 ggctggtatt ttacacgtta atctggatcg gtggtacggt tgtcttgaga tttttttcgg
     2461 agatcagtgg cagcaagggt tgacaaacta tcgaagtgtt tgtataatat ctttactgac
     2521 caggtggtca gtaaaggaga atgtatatga atgccaccgt tgaaatgacc cgagcacagc
     2581 cgtcgattac gctctcggat gtggagacaa ccggtttgct cacactctac tgccacgccc
     2641 ttgagagcca atcgactaat cctatcctca aggatgacaa ggcggttgaa atcgcccggc
     2701 agctaactcc gctgttgatc gattcaaaag acaagctact cagaaagctc gccaccaaca
     2761 aagtggatca aaggctggtc gtgcatatcg ccctgcgcgc caagcaatac gaccaatacg
     2821 ccaaaaattt ccttgatcaa aatcccgatg ggatcattgt caatatcggt tgcggcttgg
     2881 acccccgctt tttccgtatc gataacggca gcatgatctt tttcgacatc gacctccctg
     2941 aagtgatccg cttcaagaag aagattctgg cggagagtga ccgctatcgc atgatcgccg
     3001 cctcggtttt tgattaccaa tggatggatg aggtagccca atatggcagt cgtcccgtaa
     3061 tgttcctcgc cgaaggagtg ttcatgtatc tcgaggcgga aaaagtcaag tcgctggtat
     3121 tggagcttca atcccgcttc cccggctcgg aactggtatg cgaggttgtc aacgagatgt
     3181 gggtccggca gcctttgaat tacttggtga gcatcaagat gcagcggcag ctgagtttgg
     3241 gtaaggatgc ggtgtatcgc tttgggattg ccagcagccg ccagatggaa acctggcatg
     3301 cgggtatcca atatttagat gattggtcct atttcgacac tgatcatgaa aaacttggct
     3361 ggatgaagtg gatcggtaag agcgaattta tgcgcaaagt ccagtggacc gtacattacc
     3421 ggcttaatta agactctatc tgaactgcgg agacctggta ttgtacgaga actttgagag
     3481 aatcccccca gatgaacagg caaggattct cgaatcatgt cttgaggagt tcgccttgca
     3541 tgggtacgag aaggcctcga ccaatgcaat ggtcaagagg gcgaacatcc ccaaggggac
     3601 gctgtttttc tactttggca gcaaaaaaga cctctatcta tatttgatcg atcgagcagt
     3661 ggagcgcttc gtcgctttta tgagccaagc taatgacgag ctgccttcgg atctgtttga
     3721 gcgcctgtta caccgcagta aaatccgctt ccaattcgca cttcaagagc cgactcttta
     3781 ccagtttttt taccatgcat tgatcaacac ccccgagtca attcaatccg acctgcagag
     3841 ccgctattct acctacgctt tggccagcag gcagtccctc tatgagggcc tggaccgctc
     3901 gaaatttaag gatgggatcg atatcgagca ggcgatcgag ctggtttacc tggtgctgga
     3961 aggcatctta agccgctaca ctgggttgct gaagaagatc cagccagatg aagggttgca
     4021 actcgtcgag aagctcaccg acgaatgctg gggctatttc gaaatcttga agcaaggtat
     4081 gtataaatag gggaaattga ccgctaattt tgcgcaaata ggtcgactag attcccatct
     4141 ggatcgataa ctaccgcata gcgctgtccc cagaaggcgt cccatggagc cttctagctt
     4201 tggtatccac tttagataac gcggttgtac aaatcatcca ccgccgccgg gcttgcgcac
     4261 ctgaatgcca gcgtcatccg ctggcctacc actggatcaa cccaatctgg ataaatgctc
     4321 ttcaccatct ccaggctgtc ccaggcaatc cggaatccat tgggtagcgt gatctcaaca
     4381 tgctgttctg cgtccatatt cgctggaata tccagcccca gcagagtata gaatcgcagc
     4441 gagggagcca tgtcttctac acagggcaat tgcatatgcc ctgaggggca tgcacaatcc
     4501 cgtaaaaaga gtgtttgggg tattttggtg ggggcatacc cccaccaaaa taccccattt
     4561 tctctcctca gtggcgggaa atcgcccaaa tgcgattgcc ctaggtatta attattgaca
     4621 acaattaccg cattattttt agatataata caaaggattt gcccgtatag gattgcaact
     4681 atgactacag aaaaaacaga acatgatacc tttgttgaaa ctgcctggcg caacctgtta
     4741 attcaggggg aatctaatga agagaaacgc cttagaagaa tattcagaat gtttcccgcg
     4801 aaaacaaaat gtaaatggtg tgatctgcct tttgaccacc cagcttcttc gctcatccat
     4861 cttgtgttta gaaagaggcc atctgcattc aacccgcgct tttgcaacgt ttgcgatgat
     4921 ttcgctgtga aatttcaagg cggggcggaa gttgagcttt cgatggtgtt tgcggatatt
     4981 cgcggatcca ctactcttgc ggaaggaatg agtgccacag aattcaaaaa attgatcgat
     5041 cgcttttatc aagtgtcaac caaaatcttc attcagacca atgcaatgat cgacaaattg
     5101 gtcggggatg aagtaaccgc attttatcta cccggactag ccggaaagga ttatgctcat
     5161 ctggcaattg aggctgcaca agaacttttg attaaaactg gacacgaaga cccgtacgga
     5221 ccctgggcgc cgattgggat cggtgtccat accggggttg cctttgttgg ggctgtgggc
     5281 acttcagatg ggatggttga cattacggcc ttaggggatg cagtgaatat tgctgcccgg
     5341 ttagcttcgc aagcaaaagc aggtgagatt ttgcttagcg agaaaactat acaagcggca
     5401 aagttagatt catcaaatat ggaaaagaga agcctagaat taaaggggaa aagctcgact
     5461 ttcgatgtta aagtcatgcg gatcacaccg ggggactaaa ttttttagct gattgcttgc
     5521 actggatatc tcttcagatt acggttctta aaatcatcgg ctttgaccat aattttcggt
     5581 gcatggtcgc tccttttacg ttttaatcca cacacagata ggaggcgatt tggataagcg
     5641 cgattatcgt aatcaaatgg ttatgagcgt tattcctgtg cacatacatt tatgtgaaag
     5701 ctagaggaaa ttcccccatc gcaaactctg accaaccatc tttctagggc tggctatcta
     5761 tgaatgggta cacctgcgct atttcaagaa ttcttcacca ggcagggata acgaagtaat
     5821 ttaaacgaaa acgcacccag gtctgggtgc ggatcgtttc tcatccaaga ggctcctcgg
     5881 gtaggactcg aacctacaac ctagcggtta ac
//
LOCUS       DBNGSX010000373         3042 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1072341, whole genome shotgun sequence.
ACCESSION   DBNGSX010000373 DBNGSX010000000
VERSION     DBNGSX010000373.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1072341"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="AC16CB_11220"
     CDS             <1..690
                     /locus_tag="AC16CB_11220"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HIR3343083.1"
                     /translation="MYRCSGTLLSPTVLLTAGHCTNNYPDEPFTGMRVFTESDVEAGI
                     GTTNNYPYAGPNSVEAVAWAAHPLYETAPFYFHDVGVVILEEPGVILDEAKYGSLPEV
                     DQLDAYKTRRGLNPVSFTSVGYGRQESYPTPAMFLANNTRTRYVAYPRLIQINVPGFT
                     GDFSLLLSNNANTGGTCFGDSGGPNFLYDSNVVAGITSFGMNGACGGTGGVYRTDKQD
                     VQDFINSYLNP"
     gene            complement(752..1330)
                     /locus_tag="AC16CB_11225"
     CDS             complement(752..1330)
                     /locus_tag="AC16CB_11225"
                     /inference="COORDINATES: protein motif:HMM:NF047646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase"
                     /protein_id="HIR3343084.1"
                     /translation="MPYRQDIFASGQYYHIYNRGAGKGRLFFNPGNYEFLLKLVARYH
                     RNYGAGVIAYCLMPNHYHFLLRQETEEPLSKFINVLFNAYVQAVNRQQDRSGTLFEGR
                     FRHVWIDREEYLVHLCRYIHWNPVKANLVSNPEDWPYSNYLEWIGRRKGVLKDDSFIS
                     ERFHTPESYQHFVLSYPENDQENERIMKFVWN"
     gene            complement(1384..2829)
                     /locus_tag="AC16CB_11230"
     CDS             complement(1384..2829)
                     /locus_tag="AC16CB_11230"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HIR3343085.1"
                     /translation="MTAFDFLVVLPLIVLVSWATLLILADLFIPEDRKVWTVLLAALG
                     LLVTLVILIVQVKPQTVAFNGMVVVDGFAVFLSALFLGSGLVAIALSYDYLKRLGIDR
                     SEYYILLLFSISGMMLMAIAADLIVVFLALELLSIPLYVLAGFARPRPDSEEAALKYF
                     LLGAFAGGFVVYGVALVFGATGTTSLEQVVAAVKIGSANLTLLGTGAALILIGLGFKV
                     AAVPFHMWTPDVYQGAPSTVTAFMAIGAKAGGFAALMRVFVTAFPSLVADLTPILWAL
                     AALTMILGNVVAIAQGNIKRLLAYSSIAHAGYILMAIVSYGNGKVAPDAVASALFYLL
                     GYGLANFGAWAVVIALETVEQKGLALDDYAGLGRKYPGLAAAMVIFMLSFTGVPPTLG
                     FLGKFYLFRTAIEGGFIGLALIGVLTSLVSAYYYLRVVVIMYMKEGEPQVHSEPWLNL
                     TAGGAAVATVVLSIFAAPIFAWAAQAALRLF"
BASE COUNT          752 a          886 c          813 g          591 t
ORIGIN      
        1 atgtaccgct gcagtgggac attgctctcc ccaaccgtgc tcttgaccgc tggacattgc
       61 accaataact acccagacga gccatttacc ggcatgcgcg tcttcacaga atctgatgtt
      121 gaagctggga tcggcacaac caataattat ccatatgccg gccccaacag cgtggaggcg
      181 gttgcttggg ctgctcaccc cctgtatgag actgcgccct tctacttcca cgatgtgggt
      241 gtggtgatac tcgaagaacc cggcgttatc ttggatgaag ctaagtatgg ctcgcttccc
      301 gaagtagacc aactcgatgc ctataagact agacgcggcc tgaatcctgt aagctttacc
      361 tccgtcggtt acggccggca agagagctac ccgactccag ccatgttctt ggccaacaac
      421 acgagaacgc gctacgtagc gtacccgcga ctgatccaga ttaatgtgcc gggctttacg
      481 ggcgatttct cattactgct gtccaataac gccaatactg gcgggacatg cttcggtgac
      541 tcgggcggac ccaatttcct gtatgacagc aatgtggttg ccgggattac gtcattcggg
      601 atgaatggcg catgcggcgg cacgggtggg gtataccgca ccgacaaaca agatgtgcaa
      661 gattttatta attcctacct gaatccgtaa atttatagtt acaacaaagt attagataac
      721 gtcccgcagg ttccgaacct gcgggacgtt actagttcca cacaaatttc atgatccgct
      781 cgttctcttg atcattttct ggatagctta gaacaaagtg ttggtaggac tcaggtgtat
      841 ggaagcgctc gctgatgaaa gagtcatcct ttaatacccc ctttctacgc ccaatccact
      901 ccaaataatt tgaataaggc caatcttcgg ggttagatac caggtttgcc ttgaccgggt
      961 tccagtgaat gtaccggcag aggtggacca aatactcttc tcgatcaatc caaacatgcc
     1021 gaaaccgacc ttcaaacagg gttccgctgc gatcctgctg tctattcaca gcctgaacat
     1081 aggcattaaa cagcacattg ataaattttg ataatggctc ttccgtttct tgtcgtagca
     1141 gaaagtgata atggtttggc atcaggcaat aggcgataac gccagcccca tagttccgat
     1201 ggtaacgcgc aaccaatttg agcagaaatt cataattgcc aggattgaag aaaagccgcc
     1261 ctttcccagc cccgcgattg tagatgtggt aatactgacc tgaggcgaaa atatcctgtc
     1321 gatagggcat cagcagtttt aatcaaacct cccgcaggtc aaagacctgc gggacgtttt
     1381 cgatcagaac aatctcagcg ctgcctgggc cgcccaggcg aatatcggcg cggcgaagat
     1441 gctcagcacc accgtcgcca cggcagcccc tccggcggtc aggttgagcc acggctcgct
     1501 gtgcacctgc ggctcgcctt ccttcatata catgatcact acaacccgca ggtagtagta
     1561 ggccgagacc agggaggtca gcacgccgat cagcgccagg ccgatgaagc ccccttcgat
     1621 ggccgtgcgg aacaggtaaa acttgcccaa gaagcccagg gtgggcggca cgccggtaaa
     1681 gctgagcatg aaaatgacca tggccgccgc caatcccgga tatttgcgcc ccaggccagc
     1741 gtaatcatcc agcgccagcc ctttttgctc gacggtctcc agggcgatca ccaccgccca
     1801 ggcgccaaaa ttagctaaac cgtagcccag caaataaaag agggctgaag ccaccgcgtc
     1861 tggggcgacc tttccgttcc cataggagac aatggccatc aggatatagc cggcgtgggc
     1921 gatgctggag taagccagga ggcgcttgat attgccctgg gcgatagcaa ccacgttgcc
     1981 caggatcatg gtcagcgccg ccagcgccca caggatgggg gtcaggtcag ccaccagact
     2041 gggaaaagcc gtcacgaata cgcgcatcag cgcggcgaag ccgccagcct tggcgccaat
     2101 cgccatgaaa gccgtcactg tgctgggcgc gccctggtac acatctggcg tccacatgtg
     2161 gaagggcacc gccgccacct tgaagcccag cccgatcaag atcagggctg cgccggtgcc
     2221 cagcaaggtc aggttggcag agccgatctt gaccgcagcc accacttgct ctagcgaggt
     2281 ggtacccgtc gccccgaaga ccagcgccac gccatacacc acaaaacccc cggcgaaggc
     2341 gcccagcagg aagtatttca gggctgcctc ttccgaatcc ggcctggggc gggcgaagcc
     2401 cgccagcaca tacagcggga tggagagcag ctccagcgcc aggaagacta cgatcaggtc
     2461 ggcggcgatc gccatcagca tcatgccgct gatagagaag agcagcagga tgtaatactc
     2521 actgcggtcg atccccagcc gtttaaggta gtcgtaagac agggcgatgg ccaccaagcc
     2581 gctgcccaaa aacagggcag acaggaatac ggcgaatcca tccaccacca ccatgccgtt
     2641 gaaggctacg gtctggggct ttacctgtac aatcaaaatg accagggtga ccagcaggcc
     2701 cagcgccgcc agcaagacgg tccagacttt gcggtcttca gggatgaaca ggtcggccag
     2761 gatgagcagc gtggcccacg agaccagcac aatcaacggc aagacaacga gaaaatcgaa
     2821 agcggtcata gacttccttt taattgatgc agtgcaatat gcctctcagg gtatcgtcac
     2881 acacatctct agggcattgc cagcgcagcc gtctgcagcg cgctgaccag attatccacc
     2941 gccggggcca tcagcccgaa aaacggcttg ggatacaggc caatccagaa gataaagacc
     3001 aacagcggca ccagggtggc gatctcacgc cagttgaggt ct
//
LOCUS       DBNGSX010000374         3178 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_102675, whole genome shotgun sequence.
ACCESSION   DBNGSX010000374 DBNGSX010000000
VERSION     DBNGSX010000374.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_102675"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1182
                     /locus_tag="AC16CB_11235"
     CDS             82..1182
                     /locus_tag="AC16CB_11235"
                     /EC_number="6.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016851 - magnesium chelatase activity
                     [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase ATPase subunit I"
                     /protein_id="HIR3343086.1"
                     /translation="MPPIYPFTAIVAQERMRRALVLNAVDPRIGGVLIRGERGTAKST
                     AARALAALLPRVAVVTDCRFGCDPDRPTTWCTECRERVAVGEALPVELRTTPFINLPV
                     SATEDRVVGTLDIEKAIQKGERQFEPGVLASANRGLLYIDEVNLLDDHVVDVLLDSAA
                     MGMNIVEREGISFTHPARFILVGTMNPEEGDLRPQLLDRFALSVDIRGVRDARERVLI
                     MERNLAFESDPDRFREQWLLKEDELSHKIEQARTLVDQVTYSKRDLLSIAALSASLNV
                     EGHRADLVILKAARAHAAFEGRKSITDRDIALAAELALPHRIKRGPFHQAEVSIEDLE
                     ERIEQLQGASASPGEPEMVPDERQQESLEKKK"
     gene            1194..2126
                     /locus_tag="AC16CB_11240"
     CDS             1194..2126
                     /locus_tag="AC16CB_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3343087.1"
                     /translation="MEDESKQTPSQPSPQQVFDTGSANWWDGGEKVKIGPVFTPRKIE
                     NPLDKMSRRQGGRRSRTRTDRKRGRYIQARPSPGKADDLAFDATLRAAAPFQKQRGEM
                     RKRVAFAIRSDDYQKKVRVKKAANLVLFVVDASWSMAVAERMSATKGAILSLLTDAYQ
                     RRDRVGLAVFQKDRSILVLPPTNSVQLAQRALADIPVGGKTPLSAGLFLAHQVIQRER
                     LAHPDVMPLIILLTDGAGNVSMSHLPPQEESHRIAEQIADDDIRCVVINMEHAAFDQG
                     LAQTLAEHLKAPCYTLSELKAEVLYQKVREEMAG"
     gene            2187..>3178
                     /locus_tag="AC16CB_11245"
     CDS             2187..>3178
                     /locus_tag="AC16CB_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluzincin family metallopeptidase"
                     /protein_id="HIR3343088.1"
                     /translation="MIRVTKSWIPGFLTAILLSAGALSACASRNPGLSTVANTPSHTM
                     EQPAPTTPVGGASLDLTPASTADATPSLTPQPVPKTRYTLTAVLDYAAHLLVVDERID
                     YTNASPDTLNELLLVVEPLHYPGTFNLKNIAWEDGTPLEDFRRDGSLLRLPLRAPLAP
                     GDRLALSLNYELYLPSPTPSSEVRPIPFGYTARQTNLVDWYPFIPPYKAGEGWLAHPA
                     GFFGEHTAYEIAEFDVSLMLVDARSDLVVAASAPAQMEGDWYRYSFADARNFAISVSP
                     EYQVYTTTVGSTTVLSYAFAFHEQAGRAVLQTTAEALELYNRLFGPYPRSQISAV"
BASE COUNT          692 a         1004 c          861 g          621 t
ORIGIN      
        1 gcaacccatg atcccatatg caaacatcct tctcaaaaga gggtgaataa attgagttat
       61 aatgctaatc cgaggaagta catgccgcct atttatccgt ttacagctat tgttgcccag
      121 gagcgcatgc ggcgtgccct ggtgctgaac gcggtcgacc cgcgcatcgg tggtgtgctg
      181 attcgcggag agcgcggcac tgccaagtca actgcggcgc gtgccttagc agccttgctc
      241 cccagggtcg cagttgtcac cgattgccgt tttggatgtg accctgatcg cccgaccacc
      301 tggtgcactg agtgtcgtga gcgagtcgcc gttggcgaag cgctacctgt agaactgcgc
      361 accaccccat ttatcaacct gccagtctcc gccacggaag accgagtcgt aggcaccctg
      421 gatattgaaa aagccatcca gaaaggcgag cgccaatttg agcctggcgt gctggcttct
      481 gccaaccgcg gcttgctcta catcgacgaa gtaaacctgc tggacgacca tgtggtagat
      541 gtgttgctcg actcggctgc tatgggtatg aatattgtcg agcgcgaagg catctctttc
      601 acccacccgg ctcgctttat cttggttggg acgatgaacc cagaggaagg cgacctgcgc
      661 ccgcaattgc tggatcgctt tgccctgtct gtggatatcc gtggtgtacg cgacgcgcgc
      721 gagcgcgtcc tgatcatgga gcgcaatctt gccttcgaat ccgatccaga tcgcttccgc
      781 gagcagtggc tgctaaaaga agacgagcta tcccacaaga ttgagcaggc gcgcacacta
      841 gtagatcagg tgacttactc gaagcgcgac ttgctttcca tcgccgcatt gagcgcctcg
      901 ctcaacgtgg aagggcaccg tgccgacctg gtgatcttga aagctgcccg agcgcacgct
      961 gcttttgaag ggcgaaaatc cattaccgac cgcgacatcg ccctggcagc agagctggcg
     1021 ctgccgcacc gaattaagcg aggtccattc caccaggctg aggtcagcat agaggatctc
     1081 gaggagcgca ttgaacaact gcaaggcgcg tcagccagcc ccggcgaacc ggagatggtc
     1141 cccgatgaac gccagcagga aagcctcgaa aaaaaaaagt aacgacggag gtgatggagg
     1201 acgaatctaa gcaaactccc tcgcaaccgt cgccgcaaca ggtcttcgat acaggtagtg
     1261 ctaactggtg ggatggtggc gagaaagtta aaatcggccc ggtcttcacc ccccgcaaaa
     1321 ttgagaatcc attagataaa atgtcgcgcc gccagggtgg gcgccgctcg cgcacgcgca
     1381 ccgaccgcaa acgcgggcgc tacatccagg cgcgtccctc cccaggaaaa gccgacgact
     1441 tggccttcga tgccaccttg cgcgccgctg caccattcca gaaacagcgg ggtgagatgc
     1501 gcaaacgagt ggcgtttgcc atccgctccg atgactacca aaagaaagtg cgcgtcaaga
     1561 aagctgccaa cctggtgctc ttcgtggtag atgcttcctg gtccatggca gtagccgagc
     1621 gcatgtctgc caccaagggg gccatcctct ccctgttaac cgacgcctat cagcggcgtg
     1681 atcgcgtggg attagcggtc ttccagaaag atcgttccat cctggtactg ccccccacca
     1741 actccgtcca gcttgcccag cgcgccctgg ccgatatccc cgtgggcggt aagaccccgc
     1801 tctccgcggg cctgttcctg gctcatcagg tcatccagag agaacggctg gcccaccccg
     1861 atgtaatgcc cctgatcatc ctgctcaccg atggggctgg caacgtgtcc atgagccatc
     1921 tcccacccca ggaagaatct caccgtattg ccgaacagat cgctgacgac gacattcgct
     1981 gcgtggtgat caacatggaa cacgccgcat tcgaccaggg attagcccaa acgcttgccg
     2041 aacatctcaa agcaccttgc tacaccctaa gtgagctgaa agctgaggtg ctgtaccaga
     2101 aggtgagaga ggaaatggcg ggttaggtcc ccggagattt gcaaatacat catcaggaaa
     2161 ttcggaatgc atgaacgatg aaaaccatga tcagggtaac aaaatcctgg attcctggtt
     2221 tcctgacagc aatccttctc tcagcaggag ctctatcggc ttgcgccagt cgcaatcctg
     2281 gactgtccac ggttgctaac accccgtcgc acacgatgga gcaacccgca ccaaccacgc
     2341 cggtaggagg agcatccctc gatctcaccc ctgcctccac ggctgatgcc acaccctcgc
     2401 tgacacccca gcctgtccct aaaacccgct acaccctgac tgctgtatta gactacgctg
     2461 cgcacctgct tgttgttgat gagcgcatcg attataccaa cgcctcgcct gacacgctaa
     2521 acgagctgct tctggtggtg gagccactcc actacccggg caccttcaac ctcaagaaca
     2581 tcgcctggga agatggcacg ccccttgaag attttagacg cgacggcagc ctattacgcc
     2641 tgccgctgcg cgcacccctg gcaccgggcg accgcctggc gctttcgctc aactacgagc
     2701 tatatctgcc ctcccccacg ccctcctccg aggtgcggcc tatccccttc ggttacaccg
     2761 cccgccagac aaacctggtg gactggtatc cattcatccc cccatacaag gcgggagagg
     2821 gctggctggc acatccggcg ggattctttg gcgagcacac ggcttatgag atcgccgagt
     2881 tcgatgtcag cctcatgctg gtcgatgcgc gcagcgacct ggtagtggca gcctcggccc
     2941 ctgcccagat ggaaggggat tggtaccgct acagttttgc agatgcacgc aattttgcca
     3001 tatctgttag cccggagtac caggtgtaca ccaccacagt cggctcgacc acggtgctca
     3061 gctacgcctt cgcctttcac gagcaggctg gccgggcagt cctgcagacc accgccgaag
     3121 cgctggaact gtacaaccgg ctctttggac cctacccgcg ctcccagatc agcgccgt
//
LOCUS       DBNGSX010000375         3577 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1695760, whole genome shotgun sequence.
ACCESSION   DBNGSX010000375 DBNGSX010000000
VERSION     DBNGSX010000375.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1695760"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="AC16CB_11250"
     CDS             complement(<1..682)
                     /locus_tag="AC16CB_11250"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HIR3343089.1"
                     /translation="MVQFPEAEPYRIKVVEPIKLTTREQREQILQNAHYNLFSIPAED
                     VFIDLLTDSGTSAMSHYQWAGMMIGDESYAGCRNWFHLEETVKDITGYKYVVPTHQGR
                     PAENILMTMHVRPGLSAISNMFFDTTHAHVETKGGVRRELVINEGLQTEAEHPFKGNV
                     DIGKLEDTIRRLGRDQIALIMVTITCNNNGGQPVSMANLRAIRRVADQYGMPFFLDAA
                     RFAENAFFI"
     gene            859..2460
                     /gene="murJ"
                     /locus_tag="AC16CB_11255"
     CDS             859..2460
                     /gene="murJ"
                     /locus_tag="AC16CB_11255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR3343090.1"
                     /translation="MTDNPPSASRQIARAAGTVMLAFVLSNLAGLARQILVAHAFGTQ
                     AEIEAFNAANRVAETLFNLVAGGALASAFIPTFTGILTQGDKKGAWRLASSIFNLILF
                     TMVGAIVLAALFAPQITRSVLAPGFANEPAKEALTISLMRMMLPSAAIFGVSGLIMGI
                     LNAHQVFFIPALTPSMYQLGLIFGVLVLTPKMGIYGLAWGVLVGAGLHLGLQIPALLR
                     QKGQYFPTLGIEYAPVREVARLIGPRLLGVAVVQLNFWVNTRLASLQPEGSVTAIVTA
                     FALMLMPQAAIAQSVAIAAMPTLAAQYARGQLREMRRSLADSLRGVLLLSIPASLGLM
                     LLRQPVITLLYQRGAFDSRSTELVAWALLWYAAGLVGHSLVEVLARAFYALHDTRTPV
                     LVGALAMALNVIFSLLFSALFSRIGWLPHGGLALANTLATALEAVGLVYYMRRRLEGL
                     QGDFILRGSLQAAAGALVMSAGIWIWLAWAGESPAWRTTLGGLVIGGGIYSVVMWVMG
                     VKEVKGMVAMISGYIARNRKNFTPR"
     gene            complement(2447..2893)
                     /locus_tag="AC16CB_11260"
     CDS             complement(2447..2893)
                     /locus_tag="AC16CB_11260"
                     /inference="COORDINATES: protein motif:HMM:NF014711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HIR3343091.1"
                     /translation="MADLFSNYALIAGLVAWGIAQVVKVPLEYLQTRTWNWVLLLRAG
                     GMPSSHSALVTAISYATGLFIGFDQPVFALSVAVAMVVVYDATGIRRQAGKHAALINA
                     MINDLASGHPLKEEQLREVLGHTPLEAIGGVVLGLVTAQLAWFIWG"
     gene            complement(2896..3153)
                     /locus_tag="AC16CB_11265"
     CDS             complement(2896..3153)
                     /locus_tag="AC16CB_11265"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008562988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR3343092.1"
                     /translation="MTKSLSNVPTPETPVDELSYEQALAELEGIVAGLETGDHALEAS
                     LALYERGQQLVRYCEKLLEQAELKIQSLSGEDLVDFGQQSD"
     gene            complement(3150..>3577)
                     /locus_tag="AC16CB_11270"
     CDS             complement(3150..>3577)
                     /locus_tag="AC16CB_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343093.1"
                     /translation="DEALRPEPPEELPKLAERLNENLRLSGSSSAEHTFGLGCSLGFT
                     PLLVILAILLVLRVINLILAGILLVIGLLALIGGIMLLAQKARYNGIQRAYHTSVDPE
                     INQYLAQNKLSRRQFDVIVSQLLPENAPLQAFLSKQEYV"
BASE COUNT          793 a          960 c          992 g          832 t
ORIGIN      
        1 tgatgaagaa ggcattttcg gcaaagcgtg cagcatccag gaagaaaggc attccgtatt
       61 gatctgccac tcggcggata gcacgcaggt tggccatcga gaccggttgc ccgccgttgt
      121 tgttgcatgt gatggtgacc atgatcagag cgatctggtc gcggccaaga cggcggatgg
      181 tatcttccag cttaccgata tccacgttgc ctttgaaagg atgctccgcc tcagtctgca
      241 agccttcgtt tatcaccagc tcacgacgca caccaccttt cgtttccaca tgagcatggg
      301 tggtgtcgaa aaacatattc gatattgctg ataagccagg acgaacatgc attgtcatca
      361 ggatattctc ggctgggcgt ccctggtggg tcgggaccac gtatttatag ccggtgatgt
      421 ccttgacggt ctcttctagg tgaaaccagt tgcgacagcc ggcatacgat tcatcgccga
      481 tcatcatccc agcccactgg taatggctca tcgcggaagt accactatca gtaagtaaat
      541 cgatgaaaac atcctccgcc ggtatgctga aaaggttgta atgcgcattc tggagaattt
      601 gctctcgttg ctcgcgggtg gtcaatttga taggttcgac aactttgatg cggtaaggtt
      661 ctgcttcggg aaattgaacc atcgataata tctcctgtgt gataagtaaa acctgtttaa
      721 aaagagcgtt tcattataag aataatcggg ttataggaat ttgggaagta acaaaaatca
      781 tggtctgtag actagattgg aatatctaca caggaaagta ttcgataatt ctccttcaaa
      841 aggatataat caaccatcgt gacggacaat ccacccagcg ctagccggca aatcgcccgc
      901 gccgccggga cggtgatgct ggcctttgtg ctcagcaacc tggccgggct ggcccgccaa
      961 atcctggtgg cgcacgcctt tgggacgcag gccgagatag aggccttcaa cgctgccaac
     1021 cgggtagctg aaacactttt caatctggta gcgggcgggg cgctggcctc ggctttcata
     1081 ccaactttta ccgggatact tacccagggg gataaaaagg gtgcctggcg gctggcctcg
     1141 tctattttca atctaatctt attcaccatg gtcggcgcca tcgtcctagc tgcgcttttc
     1201 gccccgcaga tcacgcgctc cgtgctagca cccggcttcg ctaatgagcc agccaaagaa
     1261 gctcttacta tctcgctgat gcgcatgatg ctgccttcag cggcgatctt cggggtaagc
     1321 gggttgatca tgggcatact caacgctcac caggtttttt tcatccccgc cctaacgccc
     1381 tctatgtacc aactgggcct aattttcgga gtgttggtac tcactcccaa gatgggtatc
     1441 tacgggctgg cctggggggt gctggttggc gcaggattgc acctgggctt gcaaatcccc
     1501 gcacttctca ggcaaaaggg gcaatacttc ccaaccttgg gtattgaata cgctccagtg
     1561 agggaggtgg cgcggctaat agggccgcgg ctattggggg tggcagtggt acagctcaac
     1621 ttctgggtga atactcgctt ggcttctctg cagccagagg gcagcgtgac cgccattgtc
     1681 accgcctttg cgctgatgct catgccccag gcagccatcg ctcaatcagt agctatcgct
     1741 gccatgccca ctttggcagc gcagtatgct cgcggtcaac tgcgcgagat gcgccgctca
     1801 ttagccgata gcctgcgggg ggtactgctg ctctccatac cagccagcct gggtttgatg
     1861 ctgttgcgcc agccggttat caccctgctc taccagcgcg gcgcttttga ttcacgctcc
     1921 acggaactgg tagcctgggc gttgctatgg tatgcagccg ggctggtggg ccactccctg
     1981 gttgaagttc tggcgcgcgc cttctacgct ctgcacgata cgcgcacccc tgtcctggtg
     2041 ggcgcgttgg cgatggcgct aaacgtaatc ttcagcctgc tcttttctgc cctattctcc
     2101 cgcataggct ggttgcctca tggagggcta gccctggcaa acacgctggc gacggcttta
     2161 gaggcggttg gattggtcta ctatatgcgc cgtagactgg aggggttgca gggcgatttc
     2221 attctaagag gaagcctgca ggcagcagct ggcgcgctgg tcatgtcagc agggatatgg
     2281 atttggttgg cgtgggccgg cgagtcgcct gcctggagaa ctaccctggg aggtctggtg
     2341 ataggagggg gaatctactc tgtagtgatg tgggtaatgg gagtgaagga agtgaagggc
     2401 atggtagcta tgatcagtgg atacatcgct cgtaatcgca agaatttcac ccccagatga
     2461 accacgccaa ttgggcggtc actaacccca aaacaactcc tccaatcgcc tccaacggag
     2521 tgtgacccag cacctcgcgc aactgctcct ctttgagtgg gtgaccacta gccaggtcgt
     2581 tgatcatagc gttgatcaaa gcagcatgct tgccagcctg gcggcggatt ccggtggcat
     2641 cgtagaccac caccatcgcc accgcaaccg ataaggcaaa gaccggttgg tcaaatccaa
     2701 taaacaagcc agtggcgtag gaaatggcgg tgaccagggc tgagtgcgag ctgggcatcc
     2761 cgccagcgcg caaaagaagc acccagttcc aggttcgcgt ttgcaaatat tcaaggggga
     2821 ccttgacaac ctgggcaatc ccccaagcta ccagtccagc gatgagcgcg taatttgaaa
     2881 acaggtctgc catattcaat cggattgttg gccaaaatcg accagatcct ctccggaaag
     2941 cgattgaatt ttaagctccg cctgttccag cagtttctca caataacgca ctaattgctg
     3001 gccacgctca taaagtgcca gggaggcttc cagggcatgg tcacccgttt caagaccggc
     3061 aacaattccc tccagctcag cgagggcttg ttcgtagcta agctcatcta ccggggtctc
     3121 cggagttggt acattcgaca atgacttggt catacatact cctgtttaga aaggaaagcc
     3181 tgaagggggg cgttttcagg taaaagttgt gagacgataa cgtcaaactg gcggcgggac
     3241 agcttgtttt gagcaaggta ttggttaatc tccggatcca cactggtgtg atatgcccgc
     3301 tggataccgt tgtagcgcgc tttctgggca agcaacatga tccccccgat cagcgctagc
     3361 agccctatca caagcaggat gcccgccaga atcaaattga tcactctcag gacaagcaag
     3421 atggcgagaa tcacaaggag aggggtaaat cccaatgagc atcccagccc aaaagtatgc
     3481 tcagcgctgc ttgatccgct gagccgcagg ttttcgttca ggcgctcagc cagcttgggc
     3541 agctcctcag gtggctctgg ccttaatgct tcatcta
//
LOCUS       DBNGSX010000376         5879 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_171631, whole genome shotgun sequence.
ACCESSION   DBNGSX010000376 DBNGSX010000000
VERSION     DBNGSX010000376.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_171631"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="AC16CB_11275"
     CDS             <1..234
                     /locus_tag="AC16CB_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343094.1"
                     /translation="EGLPYYGPVLLALQPFTTAPGGVIDFGATYTDLSNLAPINALQN
                     RSIVIHGKTVGDAYVGSLPVACGQIRPEPQGNK"
     gene            397..816
                     /locus_tag="AC16CB_11280"
     CDS             397..816
                     /locus_tag="AC16CB_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343095.1"
                     /translation="MHLVKARVLIGVVFIVNVQCAVAFLLFPGWYAPLFELQGPAGEA
                     MIRGMGVLFLMWNVPYGVALWNPIRHRRALFEAVFMQAIGLAGETWIYLTSASALVIA
                     RYALTRFIIFDALGLLALIAAVWVTRGKRSMSKSQEW"
     gene            complement(914..1270)
                     /locus_tag="AC16CB_11285"
     CDS             complement(914..1270)
                     /locus_tag="AC16CB_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR3343096.1"
                     /translation="MKREIDITAYKCKKCGKLHYPFHDRCLACKTREFDKVKPQGNTK
                     LLTFTRIYNLPWGFDQRYLTIGVVEFENGVRAMGQINVDETYNLKVGMKMRPSWEPVR
                     VQYGEDVYGLMLEPMG"
     gene            complement(1267..2457)
                     /locus_tag="AC16CB_11290"
     CDS             complement(1267..2457)
                     /locus_tag="AC16CB_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase C-terminal domain-containing protein"
                     /protein_id="HIR3343097.1"
                     /translation="MRPVAVIGVGMIPFGRRDEDSLMDMLAYASLNALDDAGLGDKTV
                     DAVYVGNMGGGIIQHQTSIASSLVDRLSLLPAAAETVENGPASGGSAVKNGLLAVASG
                     YYDTVLVVGGEKMRETTGWRATDFVSTMTHPLAEYVYGITLPGLAGMFTRLYMEKYGV
                     TREHLIAIAIKNQEMGGLNPYAHVEMSITKEGIYDNSASIVNNPVVADPLHLYDACPV
                     SDGAAALILCPLDKIKNPAKTPVVIAGFGQATDTHTLQEREDPTDLKAVTLASQKALE
                     MAKLKPSDIHVAELHDAFTILEVAESEHVGFFKKGEGGKAALAGKTALGGTIPINVSG
                     GLKARGHPVGATGVAQVVDIVYQLRHELPKNRQVKNAKNGLTVNFGGFGNNVLSFVLR
                     RVEQ"
     gene            complement(2462..3151)
                     /locus_tag="AC16CB_11295"
     CDS             complement(2462..3151)
                     /locus_tag="AC16CB_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343098.1"
                     /translation="MAIDMELEAVLRPHQYEMPPYPPKVITLANSKKMVVRQIQREDV
                     PTLLKSVFPCLTIERDYYDIVSARLYGELLAWYRYRVKSEYCLVGQIDGYAVGIVNGR
                     METEKVGMSYHTLAIDRGLRIGSHLFAAKMEYHIEYLGQDEVLIVAESPIGFRRWMIE
                     YPLEKTSIPHELGGGDSYLLTRDIYLKAKPRLVVGERPVPKELMEEAEADILVVDEDV
                     IREKIAGLKGR"
     gene            complement(3173..4054)
                     /locus_tag="AC16CB_11300"
     CDS             complement(3173..4054)
                     /locus_tag="AC16CB_11300"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR3343099.1"
                     /translation="MKFIDLTIPLGVATPPWPTYEPLQVKYFKRLAPNGANGQLVTHS
                     NHLGTHLDGEIHFHTPGKDIASLEMDFLVHEAAIVDLSDICGDYDVYTSKMVEERVEV
                     REGDILIIHTGYHHFGWDMPSANEVRYMVMHPGPDREFADWAKAKKLRWIGVDCGSAD
                     HPMNTIIRDWMPRQARQAEKVFKKKFGKSLAEYFDDTKYQLMHLEMFPYGIIHAECLG
                     GEIDLLVNRRVQVGFFPWRFVDGESSIGRCVAIVDDVEYEQLMQKKAQLPKNRFGDAY
                     DPTHLERINQLSALNLS"
     gene            complement(4130..4255)
                     /locus_tag="AC16CB_11305"
     CDS             complement(4130..4255)
                     /locus_tag="AC16CB_11305"
                     /inference="COORDINATES: protein motif:HMM:NF024759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HIR3343100.1"
                     /translation="MDIEAVAKTIIDAGYRVDMIVENCILIENKTVDQLAPIHEA"
     gene            complement(4343..5218)
                     /locus_tag="AC16CB_11310"
     CDS             complement(4343..5218)
                     /locus_tag="AC16CB_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3343101.1"
                     /translation="MWVFIVRSLDASMIFTFAGLGELINQRSGVLNVGIEGIMLFGGT
                     LGFLTAQATGSYLLGFLVAIGVGGLLGLLHGFVSITLGVDQVVSGMGIWILGFGLTTY
                     IGSPHAGPLGLDRIPTILGISPFFFLGLILVVITWFVLSKTNMGLKVRSVGENPGVAE
                     VSGISVDKTRYRCVVLGGMLMGLSGGIYSLVYNPVWSYNFLMGWGFIALALVFFSMWN
                     PIILLAGSVLFGTLWQLSLNPQLVLPGVMSRYIWRTVPFVITLGTLVLMSTKWFRTRW
                     GAARPEALGQPYVKE"
     gene            complement(5199..>5879)
                     /locus_tag="AC16CB_11315"
     CDS             complement(5199..>5879)
                     /locus_tag="AC16CB_11315"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR3343102.1"
                     /translation="FNVNEIVTTMMLNFITFWLISFLIKEGGPFMKSGGEGQSFELPK
                     SIFAPLIKGVPFTIIIAIGLAVLIYYLFAKTKIGYEIKAYGLSPAAARYAGIRISMIP
                     LLAFILGGAIAGLAGYHYFAAVPGLYKIPKNYGEFGDLAFYGIICGLISQGNPLAAIP
                     VALLFGGMSAGGRFVQGQLHMNFGVDYALLGVLMISLVAFQFFYRYKFERTQTNPDKE
                     RIDVGVHR"
BASE COUNT         1404 a         1607 c         1446 g         1422 t
ORIGIN      
        1 gagggacttc cgtattatgg cccggtcctt ttggcacttc aaccgtttac aacagctcca
       61 ggtggcgtaa tcgattttgg agccacctac acagatctca gcaatcttgc tccgatcaat
      121 gctttacaga atcgttccat cgtgatccat gggaagactg ttggtgatgc ttacgtcggc
      181 tccttgccgg ttgcctgtgg gcagataagg ccagaaccgc aaggaaacaa ataatagatt
      241 aagaccagct ttccctattg gtttggggag cgctcaacaa aatgaactat cgggcggtaa
      301 tatcaccgcc cgatagccat ttaacttcac tcttgggtgt ttaagccata gactcgttca
      361 tgcagtacaa gcagaaatta cggatacaat tggcctatgc atcttgtcaa agcccgggtc
      421 ttgatcggag ttgtttttat cgtaaacgtc caatgcgcgg tagccttcct gttatttcca
      481 ggctggtatg cgccgttatt tgagctccag ggtccagcag gcgaagcgat gattcggggc
      541 atgggtgtgt tgttcctgat gtggaatgtg ccctatggcg tggcgctctg gaatcccatc
      601 cgccaccgcc gggccttgtt tgaagcggtc ttcatgcagg cgattggcct ggcaggagaa
      661 acatggattt atctgacaag cgcatctgca ctggtaatcg ctcgatacgc actcacccgg
      721 ttcatcattt tcgatgcctt gggcttgctg gcactcatcg cagccgtatg ggtaactcgg
      781 ggcaaaagat cgatgagcaa gagtcaagaa tggtaatgtg taactggaaa actgtgttta
      841 atggggactg ccagccctga tttatatggg ctggcagtcg ttttctgatc gaataggttg
      901 aattgttgcc gaattaaccc attggctcca acatcaaccc atacacatcc tcgccgtact
      961 gcacacggac aggctcccag gatggtctca tcttcatgcc gaccttcaag ttataggtct
     1021 cgtcgacgtt tatctgtccc atggcgcgca caccgttctc gaattcgacc accccaatgg
     1081 tcaggtagcg ctgatcgaac ccccagggca ggttgtagat gcgggtaaaa gtgagtaact
     1141 tcgtatttcc ctgtggtttc actttatcaa actccctggt cttacacgcc aggcagcggt
     1201 cgtgaaaggg gtagtggagt ttgccgcatt tcttgcattt ataggccgtg atgtcgattt
     1261 ctcgcttcat tgctctaccc tcctcagtac aaaggacagg acattattcc caaatccacc
     1321 aaagttgact gtcaggccat tcttagcgtt cttgacttgc ctgttcttgg gcagctcgtg
     1381 gcgcaattgg taaacgatat ccactacctg ggcaacgcca gtcgcgccga ccgggtggcc
     1441 gcgcgccttc aacccgccag agacattgat ggggattgtc ccacctagcg cagtcttgcc
     1501 cgccagtgcc gccttgcccc cttcgccctt cttgaagaat ccaacgtgct cgctttcagc
     1561 cacctccagg atggtgaacg cgtcgtgcag ctcggccacg tggatatccg atggtttgag
     1621 cttcgccatc tctaatgcct tttgggaggc cagtgtcacc gcttttaggt cggttgggtc
     1681 ttcgcgctcc tgcaaggtat gggtatcggt ggcctgacca aacccggcga tcacaaccgg
     1741 ggttttggca ggatttttga ttttatccag cgggcagagg atcaacgccg cagcaccatc
     1801 gctcactggg caggcgtcgt ataggtgcag tgggtcagcc accacggggt tgttgacgat
     1861 ggaggctgaa ttatcgtaaa tgccctcttt ggtgatggac atctccacgt gtgcgtacgg
     1921 attcagcccg cccatctcct gattcttgat ggctatggcg ataagatgct cacgggtgac
     1981 gccatatttt tccatgtaca ggcgggtgaa catgccagcc aaccccggca aggtgatgcc
     2041 ataaacatat tcagccagcg ggtgggtcat cgtggatacg aaatcggtag cgcgccagcc
     2101 tgtcgtctcg cgcattttct cgccacccac caccaagacg gtgtcataat agccagaggc
     2161 taccgccagc aagccattct taactgccga accgccagaa gcagggccgt tctccacggt
     2221 ttcagcagct gcgggcagca ggctcaagcg atctaccagc gagctggcga tcgaagtctg
     2281 gtgttggatg atgcctcccc ccatatttcc tacgtacact gcgtcgacgg ttttatctcc
     2341 caggccggca tcgtccagcg cattcagaga ggcgtaagcc agcatatcca tcaagctgtc
     2401 ttcgtcacgc cgcccaaagg ggatcatgcc aacgccgata acagctacgg gtctcattgt
     2461 ttcatctccc tttcaatccg gcaatttttt cgcggataac atcttcatcg acaaccagga
     2521 tatctgcttc agcttcttcc ataagctcct tgggcactgg gcgttcaccc accaccaatc
     2581 gtggtttggc ttttaagtag atatcacgcg ttagcaggta tgagtcccca ccacccagct
     2641 catgcgggat actcgtcttc tcgagcgggt attcgatcat ccagcgccgg aagccgatgg
     2701 ggctttctgc tacgatcagg acttcatctt gcccaaggta ctcgatatgg tattccattt
     2761 ttgcggcaaa cagatgcgag ccgatgcgca agccacggtc gatcgctaat gtatggtagc
     2821 tcataccgac tttctcggtc tccatgcgcc cattaacaat ccccaccgcg tagccatcga
     2881 tttgcccgac aagacagtac tcacttttta cccgatagcg gtaccaggct agcagctcgc
     2941 cgtacagccg tgcgctgacg atgtcgtaat aatcacgctc aatcgtcaga caggggaaaa
     3001 cagacttgag taacgttggt acatcctccc tctgtatctg gcgcaccacc attttcttgc
     3061 tatttgccag ggtgatcacc ttgggcgggt agggcggcat ttcatattga tgcggtcgca
     3121 agactgcttc cagttccata tcaattgcca cagtaaaacc tcctttaatt gattagctga
     3181 gatttaaagc actcaactgg ttgattcgct ctaaatgcgt gggatcgtaa gcatccccga
     3241 agcggttctt gggcagttga gccttttttt gcataagttg ctcatattct acatcatcta
     3301 cgatagccac gcagcgacca atggaggatt ctccatccac aaagcgccag gggaagaacc
     3361 ccacttgcac gcggcgattg acgagcaggt ctatctcgcc acccaaacac tcggcgtgaa
     3421 tgatgccata ggggaacatc tccaggtgca tgagctggta tttggtgtca tcgaagtact
     3481 cagccagtga cttgccaaac ttcttcttga ataccttctc tgcctggcga gcctgtcttg
     3541 gcatccagtc tcgaatgatg gtgttcatcg ggtggtcggc gctaccgcaa tcgaccccga
     3601 tccagcgcag ctttttggcc ttggcccagt cagcaaattc gcggtctgga ccagggtgca
     3661 tcaccatata gcgcacctcg ttcgcagagg gcatgtccca gccaaagtga tggtagccag
     3721 tatggatgat gaggatatcc ccctcgcgaa cttcgacacg ctcttcaacc attttgctgg
     3781 tgtatacatc gtaatcgccg cagatgtcgc tcagatcgac gatggcagcc tcgtgaacta
     3841 aaaaatccat ctcgagcgag gcgatatcct tcccaggtgt atgaaagtgg atttcgccat
     3901 ccaggtgggt gcccagatgg ttagagtggg tgactagctg cccattcgcc ccatttggtg
     3961 ccaggcgctt gaagtacttc acctgcagag gttcgtatgt tggccaggga ggggtagcca
     4021 ctcccagggg tatggtaagg tcaatgaatt tcatgctaaa gctccttata aatatttttt
     4081 caatcttcac cgcgaaggcg ctatgagcgc gaagattctt ctataacaat taggcctcgt
     4141 gaatcggagc aagttgatcg accgttttgt tttcgataag aatgcaattc tcgacaatca
     4201 tatccactct gtagcccgcg tctattattg tcttggcaac cgcctcaata tccatagatg
     4261 ccttcgcgtt ctttgcgcct tagcggttta ttacagaatc ctcaatgttt caaacagaat
     4321 accgatctgc atcacaccgc ctttactcct tgacgtaagg ctggccaagc gcttcaggcc
     4381 tggctgcacc ccagcgtgtc ctaaaccatt ttgttgacat aagcaccaaa gtacccaggg
     4441 taatgacaaa gggaacagtt ctccagatgt atctggacat gacacccggg agaaccaatt
     4501 gcggattgag cgaaagctgc cataacgttc caaatagcac tgaaccagcc aataggataa
     4561 tcggattcca catggagaaa aataccaggg ccaaagcgat aaaaccccaa cccatcaaga
     4621 agttatagct ccaaaccggg ttgtaaacca gcgaataaat cccaccggat aatcccatca
     4681 gcatgccacc caataccaca caacgatagc gggtcttatc aacactgatc ccggacactt
     4741 ccgcaacgcc tggattctct cccaccgacc tcaccttcag gcccatgttt gtcttggata
     4801 gaacgaacca tgtaatcaca actaaaatca gccctaggaa aaagaatggg gaaataccca
     4861 ggatagttgg aatgcgatct aatcccaaag gtccagcgtg ggggctgccg atataggtcg
     4921 tcaagccgaa acccaggatc cagatgccca tcccgcttac gacctggtca acccccagag
     4981 tgatggagac gaatccatgc agaaggccca gcaagcctcc gaccccaatg gctaccagga
     5041 aaccgagcag gtagctgccg gtcgcttggg cggtgaggaa acccaaggtg ccaccgaaaa
     5101 gcatgatacc ttcgatgcca acattcagaa ctcctgagcg ctggttgatc aattcgccga
     5161 gtccggcgaa ggtgaatatc attgatgcgt ctaggcttct aacgatgaac acccacatct
     5221 attctctcct tatctggatt tgtttgagtc cgctcaaact tgtaacggta aaagaattga
     5281 aacgcaacca gcgaaatcat tagcactccg agcagcgcat aatccacgcc aaaattcata
     5341 tgcagctgcc cttgaacgaa cctccccccg gcggacattc caccgaaaag cagtgccaca
     5401 gggatcgcag ccagagggtt cccttgagaa atcagcccac agatgatccc gtagaaagcc
     5461 aggtcgccga actccccgta gtttttggga atcttataca gaccaggaac tgcggcgaaa
     5521 tagtggtacc cagccagtcc agctatggcg ccgcccaaga taaacgccag cagagggatc
     5581 atggatattc tgatcccggc gtagcgggca gccgctgggc tgaggccgta agccttaatt
     5641 tcgtatccaa ttttcgtctt tgcgaataag tagtatatta gcactgcaag cccaatggcg
     5701 atgataatcg taaaaggaac acccttgatt agtggggcga atatggattt gggcagctca
     5761 aagctttgcc cttccccgcc ggatttcata aagggaccac cttctttaat caagaatgaa
     5821 atcagccaaa aggtgataaa gttcaacatc atcgtcgtga cgatctcgtt gacattgaa
//
LOCUS       DBNGSX010000377         1781 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_448936, whole genome shotgun sequence.
ACCESSION   DBNGSX010000377 DBNGSX010000000
VERSION     DBNGSX010000377.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_448936"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="AC16CB_11320"
     CDS             <1..302
                     /locus_tag="AC16CB_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343103.1"
                     /translation="SGPPRFTTRKLFPLLDRLLSFDTDYYLAAHESNPMSRTDMVDYA
                     HLLKTIGCVVEKIGPDRQSVLAALQTTFSQKLNDEHVEIADAFLAGLSKSTPPAE"
     gene            374..841
                     /locus_tag="AC16CB_11325"
     CDS             374..841
                     /locus_tag="AC16CB_11325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR3343104.1"
                     /translation="MKSWHDFSIEEPELAQIGQALLFRSRIGLAFLATLRKDGAPRLH
                     PVSLIISNGHLYVLIPASSPKCADLVRDGRYAMQAFPSQGEENEEFYLAGRAERIQAT
                     MDRQAVIKHTNILAGEDEVMFEILLDRAMYTKLVDYGTPDERPVHRKWRATSS"
     gene            complement(913..>1781)
                     /locus_tag="AC16CB_11330"
     CDS             complement(913..>1781)
                     /locus_tag="AC16CB_11330"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3343105.1"
                     /translation="IKGGLAALLLGLGNLPRRDLKGALFISASIGEEHMEGAALQPIV
                     ARTQPDFAVICEPTGCQLGVAQKGRTEFWIQVAGTPAHTSQPDMGDNAIYRAAEIIQD
                     LRRLPLAEDPLLGRAVMELIEIVSSPFPGNCIVPDGCRLRYDRRLLPGDTRQSLMDSL
                     HEAMANHQRWSIAFQHMTSVGFQADGLETYTGHVLEGEHFYPAWTLPEDSPWLAKARR
                     GLEMNGLTAKSMAVPYCTNASFTAGQAGIPSLIFGPSDISLAHIVDEYVEVDELMRAI
                     AGFTGLAKALAE"
BASE COUNT          409 a          479 c          476 g          417 t
ORIGIN      
        1 acagcggtcc gcccagattc actacgcgta aactatttcc gctcctcgac cgcctgctaa
       61 gttttgacac ggattactat ctcgcagcgc atgagtctaa ccctatgtcc agaacggata
      121 tggtcgacta tgcgcatcta ctcaagacca ttggttgtgt agtagaaaaa attggcccag
      181 atcgtcagtc agttcttgcc gctttgcaga cgacattcag tcagaaatta aatgacgagc
      241 atgttgaaat cgccgatgcc ttcttggcgg gcttgagcaa atccacgccc ccagcagagt
      301 gaggatggct gaagcgcaca acgggggata cttatacgct cgcgtttcgg cagcaataga
      361 ggatgatcat tacatgaaat cttggcacga cttctctatc gaggagccag aattggctca
      421 aattggccag gctcttcttt ttcgttctcg cattgggctt gcttttttgg caactctccg
      481 caaggatggc gcccctcgat tgcaccctgt cagcctgatc atctccaacg gtcatctata
      541 cgtgctcatc cctgcaagct ctccgaagtg cgccgaccta gtgcgcgatg gcagatatgc
      601 aatgcaggcg tttccttcgc agggtgagga aaatgaggaa ttttacctcg ccggtcgcgc
      661 tgagcgaatt caagctacga tggaccgcca ggcagttatc aaacatacga acattctggc
      721 aggagaagat gaagtgatgt ttgagattct gcttgaccgc gccatgtata ccaagcttgt
      781 cgattatggc acccctgacg aacgacctgt tcacaggaag tggcgggcaa caagttcata
      841 accttgttgg tcacatgaga tctagactag ctaatctata aattggagta actgacaggt
      901 catgcctgga gattactccg ccagtgcctt tgccaaaccg gtaaagccag ctatggcgcg
      961 catcagctcg tcaacttcga cgtactcgtc gacgatatgt gccaggctga tatcggatgg
     1021 accaaagatc aagctgggga tgcccgcttg cccagcagtg aagctagcgt ttgtacagta
     1081 gggcaccgcc atgcttttcg cagttaaacc gttcatctcc aatccccggc gggctttcgc
     1141 tagccaaggg gaatcttctg gcagcgtcca agccggatag aaatgctcgc cttccagcac
     1201 atgaccagtg taggtttcca gcccgtccgc ctggaaacct acactggtca tgtgctggaa
     1261 ggcgatgctc caacgttggt ggttcgccat tgcttcgtgc aggctatcca tcaggctttg
     1321 gcgggtgtct ccaggcaaaa ggcgccggtc gtaacgcagg cggcagccat cggggacgat
     1381 gcagttgccg ggaaatgggc tggacacgat ttcgatcaat tccattacag ccctacccag
     1441 caagggatct tccgctaatg gtaggcggcg taaatcctgg atgatctccg cagcacggta
     1501 aatggcgttg tcacccatat cgggttggga ggtgtgagcc ggtgtgccag ccacctgtat
     1561 ccagaattcg gtgcgcccct tttgggccac accgagctgg caaccggttg gctcgcagat
     1621 tacggcaaag tcgggctgtg tgcgggctac aatgggctgc agcgcggcac cttccatgtg
     1681 ttcttcgccg atgctggcag aaataaatag cgcgcctttt aagtcacgac gcggcaaatt
     1741 acctaagccc aacagaagag ctgctaagcc tcctttgatg t
//
LOCUS       DBNGSX010000378         3576 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_171741, whole genome shotgun sequence.
ACCESSION   DBNGSX010000378 DBNGSX010000000
VERSION     DBNGSX010000378.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_171741"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1851
                     /locus_tag="AC16CB_11335"
     CDS             <1..1851
                     /locus_tag="AC16CB_11335"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR3343106.1"
                     /translation="RNIASYNVVITAANLPDDPGEIQEIFRALSAQIDVPINLNEISE
                     ENPYVPCVSDHGIAQIVEYGESSTPFQPVRIKCDVERITAMVIQEKANDWPGVGIEIV
                     PIRDYPTGVLTASMIGFLGPIPANRAEEFISVGLDPNRDKVGYAGLELQFQDTLAGKN
                     GQRVIEVDVAGKEIRTIGETQAPVPGQNLILTLDTRLQSAATAIVQREINFWNTYFGE
                     IRSTSAVAIAMNPTTGEILAMVSYPSYENNRMARLIPAYYYNQLVQDPAIPLLNHAVG
                     DTLPAGSVFKLTTAVGALNEGVVTPEQVLETPPQITVSETFSPNEPGRPREFVDWNEA
                     GFGRLDFLGGISNSSNVYFYKLGGGYQDEVPEGLGVCRLGTYARALGYGEYPGTGLPE
                     EERGLIPDPQWKRITRGESWSTGDTYIASVGQGYVLATPLQVLLSATTIANDGKLMQP
                     TLVREIVDGEGNLVQPFEPNMRWDITQDAIIQEFEETTLRGCEPIEGRTKTVQPWVVA
                     KVQEGMRRAVTDGTLQREFVNATIAAAGKTGTAEYCDKYALARNRCIPGEWPTHAWTV
                     AYAPYENPEIAVVAFVYNGGEGASVAGPIVWQLLNAYFELKAIDVAQQAP"
     gene            1875..2555
                     /gene="minC"
                     /locus_tag="AC16CB_11340"
     CDS             1875..2555
                     /gene="minC"
                     /locus_tag="AC16CB_11340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HIR3343107.1"
                     /translation="MQPAVKIKGIREGLLIAIGEGAWDEVSTDLLAKLDEQGTFLKGA
                     RLTIDVGNRVLKAVDLGKMRDALSERGLILWAVLSNSPTTEQTAQSLGMATRLTKPHP
                     EREAKPLDTSVHSGEQAVLIHRTLRSGYSLHFNGHVVVIGDINPGAQVIASGDVVVWG
                     RLRGMVHAGAGGNTDALVCALDLAPTQLRIADQIAITPQRRGKPQPEMARLKDGQVVA
                     ETWNPKKK"
     gene            2626..3429
                     /gene="minD"
                     /locus_tag="AC16CB_11345"
     CDS             2626..3429
                     /gene="minD"
                     /locus_tag="AC16CB_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HIR3343108.1"
                     /translation="MAGKVVTVTSGKGGVGKTTATANLGAAMASDGKKVVCVDADIGL
                     RNLDVVLGLENRIVYDLVDVVEGRCRLRQALIRDKRLPELFLIPAAQTRDKSAVSPSD
                     MIRLCDDLRQEFDWILIDSPAGIERGFRNAIAPADIVLVITNPEVSAVRDADRIIGLI
                     EAEEKGPARLVINRIKPDMVRRGDMLSADDVLELLAVELVGLIPEDENVITSTNRGLP
                     IALNGKSRAGQAFRNIAKRLSGEEVPFLEMANDGNLMGRLARLFRPGGN"
     gene            3431..>3576
                     /locus_tag="AC16CB_11350"
     CDS             3431..>3576
                     /locus_tag="AC16CB_11350"
                     /inference="COORDINATES: protein motif:HMM:NF015717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HIR3343109.1"
                     /translation="MSRFFDRLLGRDQKSARQAKERMKLVLIHDRTDLTQSTMEALKD
                     ELIAV"
BASE COUNT          873 a          948 c          990 g          765 t
ORIGIN      
        1 cgcaacatcg cttcctacaa tgttgtgatt acagctgcaa acctgcctga cgaccccggc
       61 gaaatccaag agattttccg cgcgctttcg gcgcaaatcg atgtcccaat taatctaaac
      121 gagatcagcg aagaaaatcc ctacgtgcct tgtgtatccg atcacggcat tgctcagatt
      181 gtagagtatg gcgaatcatc aacaccattt caaccagtgc gcatcaagtg cgatgtcgaa
      241 cgcatcactg caatggtcat tcaggaaaag gccaacgact ggcctggcgt gggcatcgag
      301 attgtgccta tccgcgatta tcccactggc gtgctcactg cctcgatgat tggtttcctg
      361 ggacccatcc cagccaatcg ggcggaggaa tttatttcgg taggtttgga tcccaaccgt
      421 gacaaggtag gctatgcagg cctcgaactg caattccagg acaccttagc ggggaaaaat
      481 ggacagcggg ttatcgaggt tgatgtagcc ggaaaagaga tacgcaccat tggcgagact
      541 caggcacccg tccccggcca aaatctgatt ctgactctcg atacccgatt acagtctgcg
      601 gcgactgcca tcgtccaacg ggagatcaat ttctggaata catatttcgg agaaatcagg
      661 tcaaccagcg cggttgctat cgccatgaac ccaacgaccg gtgagattct cgctatggtt
      721 tcctatccat cctacgagaa caaccgaatg gcgcgcctga taccagcgta ttactataat
      781 cagttggtgc aagatcctgc catcccgctg cttaaccatg cggtgggcga cacattacca
      841 gctggctcgg tatttaaact gacaaccgca gtcggcgctt tgaacgaagg cgtggtcacc
      901 cctgaacagg tgctagagac tccgccccaa atcacagtca gcgagacctt ctctcccaac
      961 gagcctggtc gcccacgcga atttgtggac tggaacgagg ctggctttgg acgtttggac
     1021 tttttgggtg gcatctctaa ctccagtaac gtgtatttct acaagctggg tggaggctat
     1081 caggatgaag ttcctgaagg gttgggtgtc tgccgcttag gcacatacgc ccgcgcactc
     1141 ggttatggcg agtatcctgg cactggattg ccggaagagg agagaggctt gatccctgac
     1201 ccacaatgga aacgcatcac gcgcggcgaa agctggtcaa cgggtgacac ctacatcgcc
     1261 agcgtaggtc aggggtatgt actggcaact cctctgcaag tgctgctctc tgccaccacc
     1321 atcgcaaatg atggcaagct gatgcaaccg acgctggtgc gagaaatcgt agatggggaa
     1381 ggcaatctcg tccaaccctt cgagccaaac atgagatggg acatcaccca ggatgccatc
     1441 atccaggagt tcgaagaaac caccttacga ggatgcgagc caattgaggg gcgcaccaag
     1501 accgtacaac cgtgggtagt tgccaaagta caagaaggca tgcgccgagc cgtgactgat
     1561 ggaactctgc agagagagtt tgtcaacgcg acaatagctg cggcgggtaa aactggcact
     1621 gctgagtact gcgataaata tgccctggcg cgcaatcgct gcatcccggg cgaatggcct
     1681 acccatgcct ggacggtcgc ctatgctccc tatgagaacc cagaaatcgc cgtggtagca
     1741 ttcgtctata atggcggtga gggcgccagt gtcgccgggc caattgtatg gcaattgctg
     1801 aatgcttact ttgaattaaa agcaatcgat gttgcccagc aggcaccgtg agttgatgct
     1861 ataattcctg cgatatgcaa ccggcggtta aaataaaagg tattcgcgaa ggcctgctga
     1921 tcgctatcgg agagggtgct tgggatgaag tgagcactga tctcctggca aagctagatg
     1981 agcaaggtac cttcttaaaa ggcgcccgcc ttacgatcga cgtgggcaac cgggtgctca
     2041 aagcggttga tttaggcaag atgcgtgacg ctctatctga gcgcggcctg atactgtggg
     2101 cagtgctgag caactcgccc accacagagc aaaccgcaca atccctcggg atggcgaccc
     2161 gccttaccaa accacatccg gagcgagaag ccaaaccgct ggacaccagc gtacacagcg
     2221 gagagcaagc cgtattaatc caccgcacgc tgcgctcggg ttacagcctg cattttaatg
     2281 gtcatgtggt ggtcatcggg gatataaacc ccggcgcgca ggtgatcgca tctggcgatg
     2341 tggttgtctg gggcaggctg cgcggcatgg tccacgccgg ggcgggaggc aatacagacg
     2401 cactggtctg cgcgctggat ttagctccca cccaactgcg tatcgcagat caaattgcca
     2461 tcacacctca gcggcgcggc aagcctcagc ctgaaatggc acgccttaag gacgggcagg
     2521 tggttgcaga aacgtggaac cctaaaaaga agtaacctga gaagagaggg ttgaatagct
     2581 caatgctctc attctccagc atctaattta tttatcggag attttatggc agggaaagtt
     2641 gtcacagtaa catctggaaa aggaggcgtg ggcaaaacca ctgccaccgc caacttgggc
     2701 gccgctatgg catccgatgg gaaaaaggtt gtttgcgtcg atgccgatat cggtctgcgc
     2761 aacctggatg ttgtcctagg tttggaaaat cgtattgttt acgatcttgt tgatgtagtg
     2821 gaaggacgct gccgcttacg ccaggcactg atcagagata aacgcctgcc cgaactcttt
     2881 cttatccctg ctgcccaaac tcgcgataaa agtgcggtat cacccagcga tatgatccgc
     2941 ctctgcgatg acttgcgaca ggaatttgat tggatcttga tcgattctcc cgccggtatc
     3001 gaacgcggct ttcgcaacgc cattgcccct gccgacatcg tcttggtaat caccaacccc
     3061 gaggtatcgg cagtacggga tgcggatcgc atcattggct tgatcgaggc cgaggaaaaa
     3121 gggccagcac gcctggtgat caaccgcatt aaacctgaca tggtcaggcg tggggatatg
     3181 ctctctgccg acgatgtgct ggagttactc gccgtcgagc tggttggcct catacctgaa
     3241 gatgaaaatg tcatcaccag caccaaccgt gggttgccga ttgcgcttaa tggcaaaagc
     3301 cgcgcaggac aggccttccg caatattgcc aagcgtctta gtggtgaaga agtgcccttc
     3361 ttggaaatgg caaatgatgg taacttaatg gggcggctgg cacgcttatt ccgcccggga
     3421 ggcaattgag atgagccgtt tcttcgatcg tcttctcgga cgtgaccaaa agagtgccag
     3481 acaggcgaaa gagcgcatga agctggttct catccacgat cgtaccgatc tgacacagag
     3541 taccatggaa gccctcaagg atgagctgat cgccgt
//
LOCUS       DBNGSX010000379         2299 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1765062, whole genome shotgun sequence.
ACCESSION   DBNGSX010000379 DBNGSX010000000
VERSION     DBNGSX010000379.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1765062"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="AC16CB_11355"
     CDS             complement(<1..473)
                     /locus_tag="AC16CB_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343110.1"
                     /translation="MRRLHLVSLAAVWLLMMAVVACAGAPTPAAPTSPPTSAPSPTQS
                     ESISDETEDLEKPYEDFDPDNFDDPTTIDNEWLPMRPGMQHIFEGVTEEAGLTIPHRV
                     IFTVTDLTKVIDGVRTVVIWDQDFSAGALVETELAFFAQDNDGNVWRMGEYPEVY"
     gene            complement(497..>2299)
                     /locus_tag="AC16CB_11360"
     CDS             complement(497..>2299)
                     /locus_tag="AC16CB_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3343111.1"
                     /translation="SLEVRGAIVYASLIEISALLPVFFLEGLSGAFFQPLASAYVVAG
                     LVSPLVALTVTPAWVLLLLSNAPIEHRQSPIIPWMHRIYDRVLERILLTPRLAYGTVS
                     TIVLAGVVVWPFLGQELLPSFKERDFLMHWITKPGTSHPEMWRITARACRELLTIDGV
                     RNCGSHIGQALMADEPYGVYFTENWISIDPSVDYDETLAKVQEAVDGYPGLIRDVQTY
                     LKERIREVLTGSSDTVVIRIYGPDLHTLQDKADEVLAAIDGIEGIVDLKKELHMDTPQ
                     IEVEVDLEAAQRVGLKPGDIRRATAFLVQGEEVGDIFVGGKTYDVNVWSTPETRNSLT
                     DIRELLIDTPRGGRVRLADVADIRIAPVPNVIEREGQSRKIDVSANVRGRDLGSVAKD
                     VEAALQGVGFPLEYHAEVLGEFAERQAASQRILLAGSVAVIAIFFLLYTSFENWRLAT
                     LTFFTLPWALVGGVLAAYFSNGILSLGSLVGLLTILGIATRNGIMMISHFQHLEEEEG
                     ETFGPELVKRGARERIAPIMMTALTTGLALVPLAIAGNIPGQEIEYPMAIVILGGLVT
                     STLLNLLVLPTMYLRFGKSEAWQSPSQSSAGSTA"
BASE COUNT          550 a          663 c          594 g          492 t
ORIGIN      
        1 tcgtatacct ctggatactc acccatgcgc cagacattgc catcattgtc ctgcgcaaag
       61 aaggccagct ccgtctccac caacgcaccc gcgctaaagt cctgatccca aataactaca
      121 gtacgcacgc catcaatcac cttggtcagg tcagtgacag tgaatatcac gcgatgaggg
      181 atagtcagac cggcttcttc tgtgacccct tcaaagatgt gctgcatccc cggtcgcatg
      241 ggtaaccact cgttgtcgat ggtagtcgga tcatcgaaat tgtcggggtc gaagtcctca
      301 tatggtttct caagatcttc ggtctcatcg gagatggatt ccgattgtgt aggtgatggt
      361 gcagatgtgg gtggcgaggt cggtgcagcc ggggtaggcg cgccagcaca tgccacaacc
      421 gccatcatga gaagccacac tgctgctaaa gagactagat gaaggcgtcg catagtaatt
      481 cgtattcctt tcaaaattaa gctgtactgc ctgcgctaga ttgggaaggc gactgccatg
      541 cttcgctctt cccgaagcgc aaatacattg tgggtaatac cagcaaattc agcaatgttg
      601 aagtaaccag gccgcctaga atgacgatgg ccatggggta ctcaatctcc tgaccgggaa
      661 tattaccagc gatcgccagg ggtacaagag ccagccctgt agtgagagct gtcatcatga
      721 tgggggcaat tcgctcgcgc gccccgcgct taaccagctc gggaccaaag gtctcgcctt
      781 cctcctcctc cagatgttgg aagtggctga tcatcataat cccgttgcgg gtggcaatac
      841 caaggatggt tagcaacccg accaaggaac cgagcgaaag gataccgttg ctaaaatagg
      901 ctgccagcac gccacctacc aacgcccaag gcaatgtgaa gaaagtaagc gttgccaagc
      961 gccagttttc gaaagaggtg tacagaagga agaaaatcgc gattacagcc acagatccag
     1021 ccaggaggat acgctgcgaa gctgcttgcc tctcggcaaa ctcgcccaat acctcagcgt
     1081 ggtactccag cgggaacccg actccctgca gggcagcttc gacatctttc gctaccgaac
     1141 ccagatctcg cccacgcaca ttcgccgaaa catcaatttt tcgcgactgg ccttcacgct
     1201 cgatgacgtt tggcaccggc gcgatgcgta tgtcggcgac atccgctagg cgcacacgac
     1261 cacctctggg cgtgtcgatc aacagctcgc gaatatcggt caggctattg cgcgtttcgg
     1321 gtgtgctcca cacgttcaca tcataggtct ttcctccaac aaaaatgtca ccgacttctt
     1381 cgccctgcac caagaaggct gttgcccggc ggatatcgcc cggttttagc ccaacacgct
     1441 gggctgcttc caggtcaacc tcgacttcga tctgaggtgt atccatgtgg agttcctttt
     1501 tcaaatccac aatcccctcg atcccgtcaa tcgcagccag cacctcgtct gccttgtctt
     1561 gcagcgtatg cagatcaggg ccataaatgc ggatgacgac agtatcgctg gatcctgtca
     1621 gcacctcgcg gattctttct tttagatagg tttgaacgtc gcggatcaac cctgggtagc
     1681 catccaccgc ctcttgaacc ttggcaaggg tttcatcgta gtcgactgaa gggtcaatgc
     1741 tgatccagtt ctctgtaaag tagaccccat agggttcatc cgccatcagg gcttgaccga
     1801 tatgtgagcc gcaattgcgc actccatcaa ttgtcaagag ctccctgcaa gcccgagccg
     1861 ttatgcgcca catctccggg tgagatgtgc cgggtttggt gatccaatgc atcaggaagt
     1921 ctcgttcttt gaacgaaggg agcaattcct gcccgaggaa tggccaaacc accacgccag
     1981 ccaacacgat ggtgctcaca gttccatacg ccaggcgtgg tgtgagcagg attcgctcca
     2041 acaccctatc gtatatacgg tgcatccatg ggatgattgg agattggcgg tgctcaatcg
     2101 gcgcattgga taagaggagc aagacccagg ctggcgttac cgtcaacgcc accaagggag
     2161 ataccagccc cgccactaca tatgcactgg ccaatggctg gaagaacgct cccgacaagc
     2221 cctccaggaa gaagaccggc agcaaagctg agatttcgat cagcgaggca taaacgatag
     2281 caccgcgcac ttcgagcga
//
LOCUS       DBNGSX010000380         2057 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1348857, whole genome shotgun sequence.
ACCESSION   DBNGSX010000380 DBNGSX010000000
VERSION     DBNGSX010000380.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1348857"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="AC16CB_11365"
     CDS             complement(<1..599)
                     /locus_tag="AC16CB_11365"
                     /inference="COORDINATES: protein motif:HMM:NF026042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR3343112.1"
                     /translation="MVSVGRHPNITLLSYSEVEAVSGYVGNFNIVVRRKPRYIDETQC
                     TGCGKCAEVCPIEIKNPFDLGLSTRKASYRFSAQSVPGAYTIQKSGIAPCRSACPTDQ
                     RAQGYIALLRQKRYADAYWAIRREHPFPSVCGRVCNHRCEDACSRAKYDEPVNIMGLK
                     RFIAYWAFHHRDQLPSMRDKSLAGTPFQHHHPPTGKKIA"
     gene            complement(615..1208)
                     /locus_tag="AC16CB_11370"
     CDS             complement(615..1208)
                     /locus_tag="AC16CB_11370"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3343113.1"
                     /translation="MNRSKIRTGVYVCHCGTNIAANVDVKSVAEYAQELNGVILARDY
                     TYMCSDPGQDLIKQDIQERNLNRIVVASCSPLMHEPTFRRACQDAGLNPFFFQMANIR
                     EHCSWVIDDRQLATNKAKSMVEAAVRRVRYHTPLSIRNVDMTPSALIVGGGIAGIEAA
                     LRLADAGKQVYLVERESSIGGHMAQLYKTFPTLDCAA"
     gene            complement(1205..>2057)
                     /locus_tag="AC16CB_11375"
     CDS             complement(1205..>2057)
                     /locus_tag="AC16CB_11375"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3343114.1"
                     /translation="PKSIGLLHCVGSRDPEHHEYCSRVCCMYSLKLAQLIRHYVDAEV
                     YEFYRDMRTFGKDYEAFYEQVRRDGVQFIRFDEELKISQRDGQIRITATDVYTGYQNA
                     YTVDMLVLSTGLEPNADQLQVAKTFGVSLSPDGFFLEKHPKLAPVETATDGIFLAGTC
                     QAPKDIPDSVAQGGAAAAAALSLIDAGHVSLEPFTVHIIPENCGGCRTCEGLCPYGAI
                     QMVDNAERQVAYINEVLCKGCGACAAACPAGAALQNGFTYEQILAEIEGALLESLQIY
                     PVQEMPV"
BASE COUNT          495 a          509 c          515 g          538 t
ORIGIN      
        1 attgctattt tctttccagt aggcggatga tgatgttgaa atggtgtgcc tgcaagactt
       61 ttgtcgcgca tacttggaag ttggtctcga tggtgaaaag cccaataggc aataaatcgt
      121 tttaaaccca taatattgac tggctcatcg tacttggcac ggctgcacgc atcttcgcaa
      181 cgatgattgc acactcgtcc gcaaacggat ggaaatgggt gttcgcgacg aatagcccag
      241 taggcatctg catatcgctt ctgacggagg agggcgatat atccttgagc gcgctggtct
      301 gttgggcagg cgcttcggca gggtgcaata cctgatttct gaattgtgta agcccctggg
      361 accgactgtg ctgaaaaacg ataggatgct ttgcgcgtgc ttaaacccaa atcgaatggg
      421 ttcttgatct cgattgggca gacctcggcg cacttgccac agccagtaca ctgggtttca
      481 tcaatgtaac gtggttttcg gcgaacaaca atgttgaaat tgcccacata gccgctaact
      541 gcctcaacct cgctgtagct gagcagtgtt atgtttgggt ggcgtccaac ggacaccatt
      601 ttaggggtga gaattcaagc cgcacaatct agggtgggga atgtcttgta aagttgggcc
      661 atgtgcccac cgatacttga ttccctctca acgagatata cttgcttgcc ggcgtctgcc
      721 agtcgaagtg cagcttcaat accagctatg ccaccaccta ctattagagc cgatggcgtc
      781 atatccacgt ttcgaatcga taatggagtg tgatagcgca ctcttcgaac agctgcttcc
      841 accatacttt tggctttgtt cgtcgccaat tgacggtcgt cgatcaccca ggaacaatgt
      901 tcgcggatat ttgccatctg gaaaaagaat ggatttaaac ccgcatcttg gcatgcgcgg
      961 cgaaaggttg gttcatgcat gagtggtgaa catgaagcca ccacgatccg gttaagattg
     1021 cgctcttgaa tatcttgttt gattaaatcc tggcctggat ctgaacacat gtacgtgtaa
     1081 tctctggcaa ggatgacacc attaagttcc tgtgcatact cagcaaccga ttttacgtcc
     1141 acattggcag cgatattagt accgcagtga caaacgtata ccccggttct gattttcgat
     1201 cggttcatac tggcatctcc tgtacaggat agatttgcaa ggattcgagc aaggcgcctt
     1261 caatttcagc taaaatttgc tcgtaggtga atccattctg gagtgctgct cctgcagggc
     1321 aggctgctgc acaagcgcca caccccttgc agagcacttc gttgatatat gcgacttgcc
     1381 tttcagcgtt atcgaccatt tgtatagctc catacggaca tagcccttcg caggtgcggc
     1441 aacctccaca attttcggga ataatatgca ctgtgaacgg ctccagggag acgtgaccag
     1501 cgtctattaa agaaagcgcc gccgcggctg ctgcgccacc ctgagcaacg ctgtcgggga
     1561 tatctttggg tgcctggcaa gtgcctgcta aaaagatgcc atccgtggct gtctccaccg
     1621 gcgccagctt gggatgtttc tcaagaaaga agccatctgg gctgagactc acgccgaaag
     1681 ttttagcaac ctgcaattgg tcagcgttag gttccaggcc ggtgcttaag accagcatgt
     1741 ctaccgtgta cgcattttgg taacctgtgt aaacatctgt ggcggtgatt cgtatttgcc
     1801 catctcgttg gctgatctta agctcttcat caaaacggat gaactgtacg ccatcgcgac
     1861 gaacctgctc ataaaaagcc tcgtaatctt tgccaaaggt gcgcatatcg cggtaaaact
     1921 catatacttc agcgtccaca taatgacgaa tgagctgggc aagcttgagt gagtacatac
     1981 aacataccct tgaacaatat tcatggtgct cagggtcgcg gctgcccacg caatgtaata
     2041 atccaatgct ttttggc
//
LOCUS       DBNGSX010000381         9185 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_518776, whole genome shotgun sequence.
ACCESSION   DBNGSX010000381 DBNGSX010000000
VERSION     DBNGSX010000381.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9185)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9185
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_518776"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..596)
                     /locus_tag="AC16CB_11380"
     CDS             complement(165..596)
                     /locus_tag="AC16CB_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343115.1"
                     /translation="MSMDFIKENADSLARLKAFTDRVSLSQLEQRLESGWTVAATMAH
                     MAFWDQRASLLVRRWRENGVDSSLVDVDVINDASKALCHALLPKQTIQLVLESAEAID
                     REIEGISPALAEEIEAGATQFHLSRANHRDYHLDEIEAALE"
     gene            complement(593..1111)
                     /locus_tag="AC16CB_11385"
     CDS             complement(593..1111)
                     /locus_tag="AC16CB_11385"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3343116.1"
                     /translation="MDEKIEIRSLDEGDAEVFRALHLRALDELPEAFLAVYEEELCEP
                     LERVAERLRGSKPENFYLGVFVNGHLVGMVHFERPGSIKIRHRAYLSGMYVAPERRGQ
                     GLGKALLDRALAHARSLPDLEEVGLWVIIGNQQAEALYQSAGFETFCIEPRSIKIDDR
                     YYDGAGMIMRLR"
     gene            complement(1116..1487)
                     /locus_tag="AC16CB_11390"
     CDS             complement(1116..1487)
                     /locus_tag="AC16CB_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343117.1"
                     /translation="MLRRIELIHPAEKTTLPDDSIGFFPAIKLGLMGSINPKIVEAWS
                     DKLRGPGKRQSGIRRNARFYFTELGWKEVGRKVVEACQQVGQEYRVIKVKEGTVNVVW
                     RDRHYGLEVAAQPRKRRHNRR"
     gene            1912..3186
                     /gene="serS"
                     /locus_tag="AC16CB_11395"
     CDS             1912..3186
                     /gene="serS"
                     /locus_tag="AC16CB_11395"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR3343118.1"
                     /translation="MIDINLIREATDRVRQAMALRQMESTLVDQALELDEKRRLLIQE
                     VETLKAERNAASKEIGRMKDPTERQAKIEAMRLVGDRITALDESLREVEGQLDGVMAL
                     IPNIPDPSTPLGKDENENVLIKTVGELPKYSFEPKPHWELGPALGIIDFEQGVKITGS
                     RFYVLSGAGARLQRALIAWMLDLHSRQGYTEKYPPFMVKGQTLFGAGQLPKFQDNLYR
                     DHEEDLWMVPTAEVPLTGLHMDEILDESALPRYYTAYTPCFRREKMSAGRDVRGIKRG
                     HQFDKVEMYIYCKPEDSPAAFEKMVADAEQTCAQLGLPYRVKQLCTGDLGFGSTITYD
                     IEVWAPGCGEWLEVSSVSNVTEFQARRANIRYRPADGGRPRFVHTLNGSGLGMPRTLI
                     GVLENYQQDDGSVVVPEVLRPWMGGVDVIRAA"
     gene            3196..3693
                     /locus_tag="AC16CB_11400"
     CDS             3196..3693
                     /locus_tag="AC16CB_11400"
                     /inference="COORDINATES: protein motif:HMM:NF016213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HIR3343119.1"
                     /translation="MPDWINASIFALTQVIMLVGLFGLIVPIFPGTVIIWLAALGYGV
                     VSGFGTLGIVLFVIITILMLAGTLADNVFLGAGARKGGASWKTIAIALVAGVLGTLIF
                     PPFGGLIAAPLAVLLLEYQRTGNLEKAWGALRGMAAGWGMSFIVRFAIGFVMMSLWWI
                     WAWKG"
     gene            3701..4213
                     /locus_tag="AC16CB_11405"
     CDS             3701..4213
                     /locus_tag="AC16CB_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019009679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HIR3343120.1"
                     /translation="MKTLYGWYLRVDQRITHWMARYGVLLLRISLGIVFFWFGVLKFF
                     PGLSPAQDLAARTIEQLTFGLITAKVSLPILAAWECIIGLGLISGIWMRATLLLLFMQ
                     MIGTITPVFLVPQEIFLHLPYAPTLEGQYIIKNLVIISAGIVIGATVRGGGVVDDPAL
                     VRLSHKPAHR"
     gene            complement(4240..4839)
                     /locus_tag="AC16CB_11410"
     CDS             complement(4240..4839)
                     /locus_tag="AC16CB_11410"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR3343121.1"
                     /translation="MMTLDELLLELTSGDDERAEGAVRSLSGYGEAALDALRSMLASP
                     DVDRRWWAVRALAEIPGGAAIPFIIQALSDEDPAVRQCAALGLCQQPDIPDDSVAEVV
                     VPALIEAMASNDALLARLAANALTCIGAAAVPALLDLLANSPDPVRLEAARALALIGD
                     TRAIPALFEALEDDSALMEYWANEGLERMGVGMVFFQPE"
     gene            complement(4904..5119)
                     /locus_tag="AC16CB_11415"
     CDS             complement(4904..5119)
                     /locus_tag="AC16CB_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HIR3343122.1"
                     /translation="MVSQELLEILRCPNCVREKPGLLEYYKEAWLICQDCGRKYPIRE
                     DIPVMLIDEGDNWINVAKEDLPVPPPG"
     gene            complement(5208..7088)
                     /locus_tag="AC16CB_11420"
     CDS             complement(5208..7088)
                     /locus_tag="AC16CB_11420"
                     /inference="COORDINATES: protein motif:HMM:NF016202.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HIR3343123.1"
                     /translation="MATHTTTRRMSVPTPGPVPQVFIALVGGLILFFMLSLGAVITFD
                     VIHAGRVYPGVSVAGVDLSGLRPQEATTLLAQRLTYPVTGNIVLKLGDKVWVAKPAEM
                     GLFFDAQTSANVAFSTGRQGNPLRRLSEQFNSWYRGVDVSPLLVYDQRAAYRYLEAIA
                     AQVDIPTVEASLSVNGTDVVVRPGQVGRSVDIEATLAPLEGQLRSMSDGLIPVVMVET
                     PPIILDASAQAEIARKILSAPLNLIVPDAAEGDPGSWTFDQQSIAQMLSIERVETPEG
                     MRYQVGLSAENLRGFLQGVSAELDRQPANARFIFNDDSRQLEVIQSAVIGRSLNVDAS
                     IQEINQKAVQGEHNIPLVFDYTQPAVGNDATAEQLGITELTSVQNSYFYGSSSSRIQN
                     IQTAAARFHGLLVPPGATFSMADALGDVSLDNGYAEALIIFGNRTIKGVGGGVCQVST
                     TLFRTAFFGGYPVVERYPHAYRVSYYELTRSGSVDTDLAGLDATVFVPVVDFKFTNDT
                     PNWLLMETYVKPEARVLTWKFYSTSDGRTVDWDSTGLENVEEPPEPLYQENEELARGE
                     IRQVDWEAAGADVVVYRTVHRDGQVYLQDEFVTHYMPWRAVYEYGPGTKIPKDKRNND
                     ED"
     gene            complement(7123..8085)
                     /locus_tag="AC16CB_11425"
     CDS             complement(7123..8085)
                     /locus_tag="AC16CB_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343124.1"
                     /translation="MKNLSFVFILLVLAACAPVDALQLPAGEVGSLEGQSQMPVETSP
                     RIQMGDGRTVEIIGGDEDTLREFVQRWLIPVYPGAPGGVTTVRFGALPEILPVEIPIP
                     KGAHIVASVQEPNSFTQIILDVDLSSDEITAFYSQSLDGSGWQSAPQESHSGFVGSGD
                     LPARYCLESDKAYLEVWAIETSDGPTDVRLNLYASMVSNFCQASGPGLMDEGMSLIPS
                     LKAPAGTQTLGGGAGSSGDGSAYVSTDLDSSLTVEALLEHYNAQLVAGGWELVEQGTT
                     PVVAWSTWELADMDGDVWGGTLFVMKKHMTADRLFAMLSVDKTP"
     gene            8465..>9185
                     /locus_tag="AC16CB_11430"
     CDS             8465..>9185
                     /locus_tag="AC16CB_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3343125.1"
                     /translation="MKSMNLFIQSWKKPAWRGLLQSKKTRITLLVLMLIVVAGIAGYQ
                     VLLKPNSAVATPAQIQTVVARTGELVIFASGAGQVIHATEISIGFDDSGTLSEILISV
                     GEKVQAGQTLAQLQTERTEEEVALAVAQAELDVLTAQQALDEIYETAQMDAAEALKAV
                     EDAQKALSDLNNSQLAQAQALQAVAEADEALKTAYSAYNGTILSIDASVGESVGTGAI
                     ITLADLSRPVLTVYLDETDIGM"
BASE COUNT         2028 a         2721 c         2493 g         1943 t
ORIGIN      
        1 gtatgggaag acttgcgccc agtcgccatc ttaccggtgg gttactccag cgagaagccc
       61 gaaataactt cccgccgtca actttcaagc cttgtgcata ggtaagcaca tttgttcata
      121 ttgaaaacaa cttgcgtgga aagagtatta gagccttaat tgtgctattc caacgccgct
      181 tcgatctcat ccaggtggta atctcggtga tttgcccggc tgaggtggaa ctgggtggcg
      241 cccgcttcga tctcctccgc cagggcaggg gagatgccct cgatctcgcg atcaatagct
      301 tcggccgact ccaacaccag ttggattgtc tgctttggaa ggagagcatg gcagagcgct
      361 ttggaagcgt cattgatcac atccacatcc accagtgatg agtccacacc gttctcccgc
      421 cagcggcgca ccagcaagga ggcgcgctga tcccaaaagg ccatgtgcgc cattgtggcg
      481 gctaccgtcc agccgctctc caggcgctgt tccagttgcg acaggctgac tcgatcggtg
      541 aaggccttca gcctggccaa agaatcggcg ttttctttta taaaatccat gctcatctca
      601 acctcatgat catgccggca ccatcataat accggtcgtc tattttgatc gaccgcggct
      661 cgatacaaaa ggtctcgaac cccgccgatt ggtacagcgc ttccgcctgc tgattgccaa
      721 tgatgaccca caagcccacc tcttccaagt ccggcaagct gcgagcgtgg gctagtgctc
      781 gatccagcaa ggccttacca agcccttgcc cgcgcctctc cggcgcgaca tacatcccgc
      841 tgaggtatgc cctgtggcgg atttttatac tccccgggcg ctcaaagtgt accattccca
      901 ccaggtgccc gttaacaaaa actcccaggt agaaattctc cggttttgaa cctcgcaggc
      961 gttctgccac gcgctccagc ggctcgcata attcctcctc gtacaccgca aggaaggcct
     1021 caggaagctc atccaacgcc cgcaggtgga gagcccggaa aacctcggcg tcaccctcgt
     1081 ctaacgaacg aatttcgatc ttctcatcca tccaatcacc gccggttgtg cctgcgtttc
     1141 ctgggctgtg cagccacctc cagtccatag tggcgatcgc gccagaccac gttcaccgta
     1201 ccctctttga ccttgatcac gcggtactcc tgccccactt gctggcaggc ttccaccacc
     1261 ttgcgaccta cttccttcca acccagctcg gtgaagtaaa agcgtgcgtt acgccggatg
     1321 ccactctgcc gttttccagg gccgcgcagt ttatcactcc aagcctcgac aatctttgga
     1381 ttaatagatc ccatcagacc cagcttaatc gcagggaaaa acccgatgga gtcatctggt
     1441 agggtagttt tttcagcagg atggatgagt tcaatccggc ggagcataac gatcaccgtg
     1501 ctgcaagtta tatgcccgaa tagattacta ttttaccaag cattttgcgc cgcgccttga
     1561 catcccctta ttcatgggta taatcggggc atctcagtcc ggaaggacca gcgcggagcc
     1621 aagtagccgc tttccatccc agagagctgc cggtaggtgc aaggcagcag ataatcggcg
     1681 acatccactc cgccggcccg cttgggccag tcccccccgc aggggcgagg gaccctcgcc
     1741 gtaagggggc gcgggttcgc ccgttagcgc gagtcatagg ccgtttcaaa cggcgaaagc
     1801 aggtggcacc acgagcgccc ctctcgtcct gcatgggaga ggggtgtttt cattccagcg
     1861 tttagctaca cgctgatggc tgaccgctga aagctactcg gaggtgcact catgatcgac
     1921 atcaacctta ttcgcgaagc aaccgaccgc gtccgccagg caatggccct ccgccagatg
     1981 gagagcacgc tggtggacca agccctggag ctggacgaga agcgccgtct attgatccag
     2041 gaagttgaaa ccctgaaggc agagcgcaac gccgcctcca aagagatcgg gcgcatgaag
     2101 gacccaaccg agcgccaggc caaaattgaa gccatgcgcc tggttggcga tcgtattacc
     2161 gcactcgacg agagcctgcg ggaggtggaa ggccagctcg acggcgtcat ggctctgatc
     2221 cccaacatcc ccgatccatc caccccgtta ggcaaagatg agaacgaaaa cgtgctcatc
     2281 aagacggttg gcgagctccc caagtattca ttcgaaccca aaccacattg ggaactcggc
     2341 ccagccctgg ggatcatcga cttcgaacag ggggtcaaaa tcactggctc gcgcttctat
     2401 gtgctgagcg gggccggggc tcgtctgcag cgcgccctga tcgcctggat gctcgacctg
     2461 cactcccgcc agggttacac cgagaaatac ccgcccttca tggtcaaagg gcagaccctc
     2521 ttcggcgcag ggcagctccc caagtttcag gataacctgt accgggatca cgaggaagat
     2581 ctgtggatgg tgcccaccgc cgaggtgccc ctcaccggct tgcacatgga tgaaatcctg
     2641 gacgaaagcg ccctgccgcg ctattacacc gcctacacgc cctgcttccg ccgcgagaag
     2701 atgagtgccg ggcgcgatgt gcgcggcatc aagcgcggcc accagttcga caaggtagag
     2761 atgtacatct actgcaagcc cgaagactcg ccagccgcct tcgagaagat ggtcgccgac
     2821 gctgagcaga cctgcgccca gctgggcttg ccttatcgcg tcaagcagct ctgcaccggt
     2881 gacctgggct tcggttcaac cattacctac gacatcgagg tgtgggcacc gggctgcggg
     2941 gaatggctgg aggtctcgtc ggtctcgaat gtgaccgaat tccaggcgcg gcgcgccaat
     3001 atccgttacc gcccggcaga tggcggtcgc ccgcgctttg tgcacaccct gaacggctcg
     3061 gggttgggaa tgccccgcac cctgatcggc gtgttggaga actatcaaca ggatgacggt
     3121 tcggtggtgg tgcctgaagt gctgcgcccc tggatgggtg gcgttgacgt gattcgcgcc
     3181 gcatgaggat acaccatgcc tgactggatc aacgcttcca tcttcgccct gacccaggtc
     3241 atcatgctgg tagggttatt cggcttgatc gtgcccatct ttcctggcac agtcataatc
     3301 tggttagcag ccttgggata cggtgtggtc tccggatttg gcacgctggg gatcgtttta
     3361 ttcgtcatca tcaccattct gatgctggcc gggacgctag cggataatgt cttcttggga
     3421 gcaggggccc gtaaaggagg cgcttcctgg aagaccatcg ctatcgccct ggtggctggc
     3481 gtactgggta cattgatttt cccgccattt ggcgggctga tcgccgcacc gctggcagtt
     3541 ctgctgctcg agtaccagcg cacaggcaac ctggagaaag cctggggggc gctgcgcggc
     3601 atggctgcag gttggggtat gtccttcatc gtgcgcttcg ccatcggttt cgtgatgatg
     3661 tccttgtggt ggatctgggc gtggaaagga taaaagaacg atgaaaacat tgtatggctg
     3721 gtatctgcgt gtggaccagc gtatcaccca ttggatggcc cgctatggcg tcctgcttct
     3781 gcggatcagt ctgggaatcg tcttcttctg gtttggcgtg ctgaaattct tccctggtct
     3841 cagtccggct caagacctgg cagcgcgcac cattgaacag ctgaccttcg gcttgataac
     3901 tgccaaggtg tcactaccga tcctggcggc ttgggaatgt atcatcgggt taggcctgat
     3961 ctcaggcatc tggatgcggg ctacccttct attgctattc atgcaaatga taggaaccat
     4021 cacgccggtc ttcttagtcc cccaagaaat ctttctgcat ctcccttatg cgcccactct
     4081 ggagggccag tacatcataa aaaacctggt cattatcagt gcgggtattg tcattggcgc
     4141 caccgtgaga gggggcggag tggttgacga cccggcgctg gtgcgccttt cacacaaacc
     4201 tgcgcaccga tgaactaact tgggagatgc tccgaagaat tactctggtt gaaagaacac
     4261 catccctacc cccattcttt ccaggccttc attagcccag tattccatca gggcagagtc
     4321 gtcttccaag gcttcaaaga gcgccgggat agcgcgcgtg tcgccgatca aagccagcgc
     4381 ccgggcagcc tctaagcgca cggggtcagg tgagttcgcc agcaggtcaa gcagggctgg
     4441 cacagccgct gcgccaatac acgtcagagc gttagctgcc agcctggcaa gcaaggcgtc
     4501 attcgaagcc attgcctcga tcaaggctgg cactactacc tctgccacac tatcatctgg
     4561 tatatcgggc tgctggcaca aacccagggc agcgcactgg cgtacagccg ggtcttcatc
     4621 gctgagcgcc tggattatga aaggaatagc tgccccgcca gggatttctg ccagtgcgcg
     4681 caccgcccac cagcgcctat cgacatccgg ggaggctaac atggagcgca atgcatccag
     4741 ggcagcttct ccatagccag aaagactcct gaccgctccc tcggcgcgct catcatcccc
     4801 gctggttagc tcaagtaaaa gctcgtccag tgtcatcata aaaaaacggc tctggtcccc
     4861 aaataggttc gcccgcagct taacttcagg cggacaaaac gagttatccc ggtggcggga
     4921 caggcaaatc ttccttcgcc acgttgatcc aattatcccc ttcgtcaatg agcatcacgg
     4981 ggatatcttc gcggatgggg tatttccgcc cgcaatcctg gcaaattaac caggcctctt
     5041 tatagtactc cagcaaaccg ggtttttcac gtacgcaatt gggacagcgc agaatttcta
     5101 gcagttcttg gctaaccatg attcgctcct gtatgcccac tcagggaaag aatttgtctt
     5161 ccccacacat ggagaagaga tcgggtgtaa gagggtgaga actagcctca atcctcatca
     5221 ttgtttcgtt tatccttggg aatcttcgtc ccggggccat attcgtaaac tgcccgccag
     5281 ggcatataat gagtgacgaa ctcgtcctgt aagtatactt gaccgtcgcg atggacggta
     5341 cggtatacga cgacgtcagc accagccgcc tcccaatcca cctggcgaat ctcgccgcgc
     5401 gccagttctt cgttctcctg gtacaacggc tcgggtggct cctcaacgtt ctccaacccg
     5461 gtggaatccc aatcgacggt gcgcccgtcg gatgtagagt agaacttcca ggtcaagaca
     5521 cgcgcctctg gcttgacata agtctccatc agcaaccagt tgggcgtgtc gttggtgaac
     5581 ttaaagtcca ccactggcac aaacacggta gcgtccaatc ctgccaggtc ggtatccacg
     5641 ctcccgctgc gggttagctc ataatacgag acgcgatagg cgtgtgggta ccgctcaaca
     5701 accgggtaac ccccgaagaa agccgtgcgg aacaacgtgg tgctcacctg gcaaacccca
     5761 ccgccgacgc ccttgatggt gcggttgccg aaaatgatca gcgcctcggc gtaaccattg
     5821 tccaggctca catcccccag ggcatcggcc atcgagaagg tcgcgcctgg cggtaccagc
     5881 aagccatgga aacgagcggc tgcggtctgg atattctgga tgcgtgacga gctggagccg
     5941 taaaaatacg agttctgcac gctggtcagc tccgtgatgc ccaactgctc ggcggtagca
     6001 tcgttgccca ccgccggctg ggtgtaatcg aagaccagcg ggatattgtg ctcgccttgc
     6061 actgccttct ggttgatctc ctggatcgag gcatccacgt ttagagagcg cccaatcact
     6121 gcagattgga tcacctccag ctggcgcgaa tcgtcattaa agataaaacg agcgtttgcg
     6181 ggctggcgat ccagctcagc gctgacgcct tgcagaaagc cgcgcaggtt ctctgcgctc
     6241 aggcccacct ggtaacgcat tccctcgggc gtttcaacgc gctcgatgct cagcatctgg
     6301 gcaatagatt gctggtcgaa agtccacgag ccaggatcgc cttcggcggc atcaggcacg
     6361 atcaagttga gcggcgcgct gaggattttg cgggcgatct ccgcttgggc gcttgcatct
     6421 aggatgatag gcggggtctc caccatcacc accgggatca gcccatcact catgctgcgc
     6481 aactggcctt ctaggggtgc cagggtagct tcgatgtcta cactgcgccc cacctgccca
     6541 ggtcgcacca ccacgtcggt gccattcacc gaaagcgagg cctccactgt tgggatatcc
     6601 acttgagccg cgatagcctc caaatagcgg taggcagccc gctggtcata caccaatagg
     6661 ggtgatacgt ccacgccccg ataccatgaa ttgaactgtt cgctcagcct gcgcaatggg
     6721 ttgccttgcc ggccagtaga gaaagccaca tttgcagatg tctgcgcgtc gaagaaaagg
     6781 cccatttccg caggcttagc cacccagact ttgtctccaa gtttgaggac gatgtttccg
     6841 gtcaccgggt aagtcaagcg ctgagcaagc aaagtggtag cttcctgcgg gcgcaagccc
     6901 gaaaggtcta ctcccgccac ggatacccca ggatacaccc taccagcatg gattacatca
     6961 aaggtgataa ccgcgcccag ggacaacata aaaaacagga tcaatccgcc gaccagggcg
     7021 atgaaaacct gcggtacagg tccgggggtg ggcacgctca tgcgccgggt tgtcgtatgc
     7081 gtagccattg gggggtattg taccaaataa aaatcgcatc gcctaaggcg tcttgtcgac
     7141 acttaacatg gcaaagaggc gatctgctgt catgtgcttc ttcatgacaa acagcgtgcc
     7201 tccccacaca tcaccatcca tgtccgccag ttcccaagtg ctccaggcaa ccaccggcgt
     7261 ggtcccttgc tcaaccagct cccacccacc tgcgactaac tgagcgttat agtgctccaa
     7321 tagcgcttcg accgtaaggc tggaatcgag atcagtagag acgtatgcgc tcccgtcgcc
     7381 actgctgcct gcacccccgc cgagcgtctg cgttccagca ggcgctttca gggatgggat
     7441 cagactcatc ccctcgtcca tcaagcctgg accactcgcc tggcagaaat tactaaccat
     7501 cgaggcgtat aggttcaggc gcacgtccgt cggcccatcg gatgtttcta tagcccaaac
     7561 ctccaaatag gccttatcgg attctaggca gtagcgcgcc ggtaaatccc ctgaaccgac
     7621 gaaaccacta tgtgattctt ggggggcaga ttgccatcca gatccatcca agctttgcga
     7681 gtagaacgca gtgatctcat ccgaggacag gtccacgtcc aggataattt gggtgaaact
     7741 attaggctcc tgcactgagg cgacgatgtg tgccccctta gggatgggga tctccaccgg
     7801 caaaatctcc ggcaatgccc caaacctgac cgtggtcaca cccccaggcg cgcctgggta
     7861 aaccgggata agccagcgtt ggacgaactc gcggagcgta tcctcatctc cgccgatgat
     7921 ctccactgtt ctaccatcac ccatttgaat acgcgggcta gtctctactg gcatctggct
     7981 ttggccctca agagagccca cctccccagc gggtaattga agagcatcga ccggcgcaca
     8041 cgcagccaaa accaatagga tgaatacaaa gcttaagttt ttcacgacat gcctcctcat
     8101 agcctcaagg cgtttaccga aaagttatga tatctgtgtt aacctgtgtt ctgccgttgt
     8161 ttattcgccc gattataaat aatcatgttt gaacatgagg ttcgcgatgt tagggaagca
     8221 aaaggtttag gcgggtaagg ccgcccgtat cccaatcacc tccccaactg cgtaacactg
     8281 tttcattaac gttgttctgt gtgaaatggt tcccgggcaa acatagactg gggatccgag
     8341 gatgagtatg ggtatgtaac gaatccaaat aatatccaaa tcgcatcttc ataatttctg
     8401 aacatcttcc caatatactg aggagcgaag cgaaaccagt gcccataata tggggagggt
     8461 agacatgaaa tcgatgaact tatttatcca atcctggaag aaaccggcgt ggagagggct
     8521 gctccagagc aagaaaacca ggatcactct tttggtcttg atgctaattg ttgtagctgg
     8581 catcgctgga tatcaggtat tgctcaaacc caattctgca gtagccacac cagcccagat
     8641 acagacggtt gtagcccgca cgggggagtt ggtcatattt gcctcaggag ccgggcaggt
     8701 tattcatgcc accgagatca gcatcggctt cgacgatagc ggcacgctca gcgagatcct
     8761 gataagtgtg ggcgaaaaag tgcaggcggg gcagaccctt gctcagctgc aaactgagag
     8821 gaccgaggaa gaagtcgctc tggcagttgc tcaggcagag ctggacgtgc tgaccgccca
     8881 gcaagcgctg gacgagattt acgagacggc tcaaatggat gcagccgagg cgttaaaggc
     8941 ggttgaagat gcacaaaaag ccctatcgga tctaaacaac agtcaattgg cgcaggccca
     9001 agcgctccag gcggttgcag aagctgatga ggctcttaaa accgcatact cggcatacaa
     9061 cggcacgatt ctctcaatcg acgccagcgt cggcgaatca gtcggaacag gggcgatcat
     9121 caccctggca gatctcagcc ggcccgtcct cacggtttac ctggacgaga ccgatattgg
     9181 catgg
//
LOCUS       DBNGSX010000382         5310 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1557209, whole genome shotgun sequence.
ACCESSION   DBNGSX010000382 DBNGSX010000000
VERSION     DBNGSX010000382.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1557209"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="AC16CB_11435"
     CDS             complement(<1..677)
                     /locus_tag="AC16CB_11435"
                     /inference="COORDINATES: protein motif:HMM:NF014826.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR3343126.1"
                     /translation="MFVHLRTHSYFSFLQGVPSPEDLAQAAANAGMPAQALTDFNALS
                     GAVEYYDACRAAGVKPILGLEIDISPPPGLAAAPYQAGRLAFLAIDMEGWSSLCRMSS
                     ALLTEPDLEKRTVFPFDRLTEYAPGVLCLSGGSRGLAALFAQGGLEREAAALLGALSE
                     IFSKRLYVELQMHSRTDELWVQSLVSLAGRLDLPVVATHDVHYLNPDQAHLQRVVTAI
                     RLNKPLE"
     gene            complement(692..1153)
                     /locus_tag="AC16CB_11440"
     CDS             complement(692..1153)
                     /locus_tag="AC16CB_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343127.1"
                     /translation="MRINKSINGVNHFRVRLGMGITILGLLIYLLGVDPALFGLDRSP
                     VMGFVQIATLLVGLAILCLSGFMTLNSLWNGSQKSISADIGLRLVATGYLVVVASSMA
                     DLLGFGNHTFPAIPYLGPLQAIGVIIGEIAIVAGFIMMVPYPRRDQKTGLV"
     gene            complement(1150..2238)
                     /locus_tag="AC16CB_11445"
     CDS             complement(1150..2238)
                     /locus_tag="AC16CB_11445"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HIR3343128.1"
                     /translation="MLIVDAHQDLAWNTLTFGRDYTLPVSETRLREQGSVAVEHNGDT
                     LLGWPEYQRGQVGVIFSSLFASPARLRSGDWDSQTYRDFMEANARYRAQLDVYHRLVD
                     QHSDKFRLVKGRNDLEEVVDHWKQNTAQEHPIGLVILMEGAEGVQEPSELGEWWDGGV
                     RLIGPAWAGNRYCGGTREPGPLTSEGYALLDGMADLGFILDISHMDEQAALEALDHYP
                     STIVATHANAITLLKGLDTNRHLSDRVIRMLIERGGVIGIVPVNPFLRPGWRRSDSRQ
                     EVTLHHVVAQIDYICQLAGNARHVGLGTDYDGGFGLQSVPTGIETIADLQNLSPILAE
                     KGYTEDDTAAILGENWLSILRRGLPASS"
     gene            complement(2232..3308)
                     /gene="mutY"
                     /locus_tag="AC16CB_11450"
     CDS             complement(2232..3308)
                     /gene="mutY"
                     /locus_tag="AC16CB_11450"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011243168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HIR3343129.1"
                     /translation="MAFLFTENLLDWYANNARDLPWRGHCDPYAIWVSEIMLQQTRVE
                     TVIPYFERWMQRFPTIAALAAADQQDVLAAWEGLGYYSRARNLHKAAGVVVDQHGGVL
                     PSDVTALRRLPGIGRYSAGAIASIAFGQDEPALDGNIRRVLARYFDVSEPARATSGER
                     RLWALAAMHLPTGRAADYNQAIMDLGATICTARAPDCPRCPLREGCRARLLGVQEERP
                     VLAKKTSQPHHIVTAAVIRQDGRILIAQRPARGLLGGMWEFPGGKLQPGEDLPTCLQR
                     EIREELGAQIMIGGRLGVYQHAYTHFRVTLHAFECSLKGASLPQPREHAGLRWVIPAQ
                     LVDYPMGKIDRRIATHIIESEAEC"
     gene            complement(3368..3670)
                     /locus_tag="AC16CB_11455"
     CDS             complement(3368..3670)
                     /locus_tag="AC16CB_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343130.1"
                     /translation="MSVKLLMTWDILPGREQEYFEFVVRDWIPGIQRLGLEPSDAWFT
                     MYGDQPQILAAAQSSSLSSLQHILNSPDWKTLTQQLLDYVEDFNYKVVSARTGFQL"
     gene            complement(3685..4332)
                     /locus_tag="AC16CB_11460"
     CDS             complement(3685..4332)
                     /locus_tag="AC16CB_11460"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3343131.1"
                     /translation="MEIAWYGHSCFRLTERGMATVVTDPFDHEVAGYEVLKLKADIVT
                     VSHDAPGHNFVSAVKGKSHILTGPGEYEIGGVFITGVQTNGYSKRTTDDPRNSLYVFD
                     YNGINVAHLGDLRRVPSQTEIEALGTVDVALVPVGGGGGLNAAKAAEVVSLLEPGIVI
                     PMHFSTPASLLKLAPLSKFLKEMGLGKTEQQDSLKVTRTSVPEETRVVVLNYVRS"
     gene            4385..5140
                     /locus_tag="AC16CB_11465"
     CDS             4385..5140
                     /locus_tag="AC16CB_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343132.1"
                     /translation="MPVLNPRQTPGSQVIPLPSGGWRLEIPAGPAGRYRLAQLDDYSG
                     LSRRDFPWHPPVSLTIACRASGDTIPGTWGFGFWNDPFSLSLGFGGTNRFPTLPNAAW
                     FFFASPPNYLTVRDDLPAQGGLSATFQSKPWNAALIALGAPLLPLLALPPLARLLRRL
                     GRRIVKQDAANLPIDPMEWHNYQLEWDKNGVTFCLDGRVVLQSSLAPFEPLGLVIWLD
                     NQYAAWPPTGRLSFGTLANSQAAWIELGPVTVS"
BASE COUNT         1265 a         1544 c         1353 g         1148 t
ORIGIN      
        1 tcctcaagcg gtttgttgag gcggatcgct gtgaccactc gctgcaaatg cgcctgatcc
       61 gggttgagat aatgcacgtc atgggtagcc acgacaggca aatcaagtcg tcccgccagg
      121 gaaaccaggg attgaaccca taactcatct gtgcgggagt gcatctgcaa ctccacatat
      181 agccgttttg aaaatatttc gctcagagcg cctaacaggg cagcagcctc gcgttccaac
      241 ccgccttgag cgaacagggc tgccaaaccc ctgctacctc ctgaaaggca gagcacaccc
      301 ggagcatact ccgtcaagcg gtcaaacgga aatacggtcc tcttctcaag gtctggctcg
      361 gtaagcagcg cactgctcat ccggcacaag ctgctccacc cctccatatc gatcgccaga
      421 aaggccaacc tgcctgcctg gtaaggggca gctgccaagc caggaggcgg gctgatatca
      481 atttccagcc ccaagatggg tttcacacct gctgccctgc aagcatcgta gtattccacc
      541 gcaccgctca gcgcgttgaa atcagtcaaa gcctgcgcag gcatgccagc atttgcagct
      601 gcctgagcta aatcttcggg agaagggacg ccttgcagaa aggagaaata tgaatgcgtg
      661 cggagatgga caaacaacgg agacgaataa gctagaccag gcctgtcttt tgatctcgcc
      721 gtggatatgg gaccatcatg atgaagcctg cgacaatggc aatttcacca atgatcactc
      781 caatggcttg caatgggcct aaatagggta ttgcgggaaa agtgtgattg ccaaagccga
      841 gcaagtcagc catgcttgat gccactacca ccaagtatcc ggtagccacc aagcgcagac
      901 ctatatctgc cgaaatagat ttttgcgatc cattccacaa ggaatttagc gtcataaacc
      961 cgctcaggca gaggatcgcc agacctacca ataaagtagc gatctgcaca aagcccatca
     1021 ccggactgcg atccaaacca aataatgctg gatcaacacc gagcagataa atcaaaaggc
     1081 caaggatggt aatacccatc cccaatcgca cgcgaaagtg attgactccg ttaatagatt
     1141 tatttattct catgagctag ctggtaaacc gcgccttagg atagataacc agttctctcc
     1201 aaggatcgcc gcagtgtcgt cttcagtata ccccttttct gctaagatgg gggataaatt
     1261 ctgcaaatca gcgatcgttt caatcccagt tggtacagat tgtaggccga atccaccgtc
     1321 ataatcggta cccaggccaa catgtctggc attcccggct agctggcaga tatagtcgat
     1381 ctgggcaacc acgtggtgca atgtgacctc ctggcgcgag tcgctgcgtc gccagccggg
     1441 ccgtaagaag gggttcacgg ggacaatgcc gatcacaccg ccacgctcga tcagcatacg
     1501 aattacccga tcgctgaggt ggcggttggt atccaagcct ttgagcaagg tgatggcgtt
     1561 ggcatgcgtc gccacgatcg ttgaggggta atgatccaga gcctctagcg cggcctgttc
     1621 atccatgtgg ctgatatcca agatgaaacc cagatctgcc ataccatcca gtaaagcata
     1681 tccttccgag gtgagcggtc cgggctcgcg tgtaccgccg caataacgat tacccgccca
     1741 ggcaggtccg atcaagcgaa ccccaccatc ccaccattcc cccagctcag acggctcctg
     1801 cacgccttca gcaccttcca tgagaatgac cagcccgatg ggatgttcct gtgctgtgtt
     1861 ttgcttccaa tgatcgacca cctcttctaa atcattccgc cctttcacaa gacggaattt
     1921 atcggagtgc tggtcaacaa ggcggtggta gacgtccaat tgggcgcgat agcgtgcatt
     1981 cgcctccatg aaatcgcgat aagtttggga gtcccagtcg cccgagcgga gacgggctgg
     2041 agaggcaaac aatgaagaga aaatcacgcc cacctgcccg cgctggtatt ccggccagcc
     2101 cagcagtgtg tctccattgt gttccacagc tacactacct tgctcgcgaa gacgggtttc
     2161 agaaacgggc agtgtataat cacgcccaaa ggttagcgta ttccaggcca gatcctggtg
     2221 ggcatcgacg atcaacattc ggcttcactt tcgatgatgt gggttgcaat cctgcggtca
     2281 atcttcccca tcggataatc cacaagctga gcaggaatga cccatctaag gccagcgtgc
     2341 tctcgaggtt gcggaaggct agcgcccttc aaggaacatt caaaggcatg cagggtcact
     2401 ctgaagtggg tgtaagcgtg ctggtacaca cccagccgcc caccaatcat gatttgcgca
     2461 cccaattcct cccgaatctc acgctgcagg caagttggca ggtcttctcc gggctgcagc
     2521 ttgccgcctg ggaattccca catgcctccc aataatccgc gtgcaggtcg ctgggcaatt
     2581 aatatccgcc catcctggcg tatgactgcc gcagtgacaa tgtgatgcgg ttggcttgtc
     2641 ttcttggcca ggacaggtcg ctcttcctgc acacccagga ggcgagcacg acaaccctcc
     2701 ctcaaggggc aacgcgggca gtccggagcg cgcgccgtac aaatcgtggc gcccaggtcc
     2761 attatcgcct ggttataatc cgcagcgcgt cccgtaggta ggtgcatggc agccagtgcc
     2821 cacaggcggc gctccccgct ggtcgcacga gccggctcgc tgacatcgaa gtagcgcgcc
     2881 agcacacgcc gaatattgcc gtctaaggcg ggctcgtctt gcccaaaagc aatcgaagca
     2941 atggcgcccg cgctgtagcg cccaatgccg ggaagccgtc gtaaagcggt cacatctgat
     3001 ggcagcactc caccatgttg atcaacaacc actccggcag ccttatgcaa gttgcgagcc
     3061 cggctatagt aacctaaacc ctcccaagca gccaatacat cttgctggtc ggctgcagcc
     3121 agggcagcaa tcgtggggaa gcgttgcatc cagcgctcga aataggggat aactgtctca
     3181 accctcgtct gctgaagcat gatctctgaa acccagatgg cgtatggatc gcaatgcccg
     3241 cgccagggaa ggtcgcgggc gttatttgca taccagtcca gaaggttttc ggtaaacagg
     3301 aaagccataa cacaggattg ttggtcttga ccaaacgcca agcccaacaa tcagcacaca
     3361 gccaatctca caattggaaa ccggtccgtg ccgataccac cttgtaattg aaatcttcta
     3421 cgtaatccag cagctgttgg gtcagggtct tccaatccgg agaattcaat atgtgctgca
     3481 gagagcttaa gctggatgat tgcgccgcgg caaggatttg aggttggtcg ccgtacattg
     3541 taaaccaagc atcactaggt tccagaccca gtcgctggat gccaggaatc caatctcgca
     3601 ctacaaactc aaaatactct tgctctctgc ccggaaggat atcccaggtc attaggagtt
     3661 taacagacat gatgggtctc ctattcaaga acgcacataa ttcagcacca ccacgcgggt
     3721 ctcttcaggc acagatgtcc gcgtgacttt cagggaatcc tgttgttcag ttttacccag
     3781 ccccatctct ttcaggaact tgctcaaggg ggcaagtttg agcaggctgg caggggtgct
     3841 gaaatgcatg ggaatgacaa tccccggttc caacaaactg accacttcag cggctttggc
     3901 tgcgtttaag ccccctccac caccaacagg caccaaagct acgtcaaccg tacccagtgc
     3961 ttcgatttcg gtctgagagg ggacacggcg caaatccccc aaatgggcaa cattgatacc
     4021 attataatca aaaacataga gactgttgcg tggatcgtct gtcgtgcgct tgctgtaccc
     4081 attggtctgc acaccggtaa taaaaacgcc cccgatctca tattcgcccg gtccggtaag
     4141 gatgtgcgac ttacccttta cggcagagac gaaattatgc ccaggcgcat cgtgactgac
     4201 tgttacgata tcggctttca atttcaggac ttcataaccg gcaacctcgt gatcgaatgg
     4261 atctgtgacc actgtcgcca taccgcgctc tgtaagccga aaacatgaat gcccatacca
     4321 agctatttcc ataaaacctc cacgtcagcg ttgattatac tatagccacc gttcggagta
     4381 gaatatgcct gtgctaaatc ctcgccaaac tccaggctca caggtcatac ccttgccatc
     4441 cggtggttgg cggctggaga ttcccgccgg gccggcaggg cgctaccggt tggcgcagtt
     4501 agacgattat tccggtctat cacgccggga tttcccctgg catccgcccg tctctcttac
     4561 gattgcctgc cgcgcttcag gagatactat cccaggcacc tggggatttg gcttttggaa
     4621 tgatccattc agcctctctc tgggatttgg cggtacaaac cgatttccga cactgcccaa
     4681 cgccgcatgg tttttcttcg cttccccccc caactatctc actgtgcgcg atgatttgcc
     4741 tgctcaagga ggcctgtcag ctacctttca atccaaaccc tggaacgcgg ctttaatcgc
     4801 tttgggagcg cctttgctcc ccttactggc actgcccccc ttggcgcgcc tgcttcggcg
     4861 actcggaaga cgcatcgtga aacaggatgc agccaatctg ccaatcgacc ctatggaatg
     4921 gcacaactac cagctggaat gggataaaaa tggcgtaacc ttctgcttgg atgggagggt
     4981 agtgctccaa tcctccctgg cgccattcga gccgcttggg ctggtgatct ggttggacaa
     5041 ccaatacgct gcctggcctc cgactggacg cttgagcttt ggcactctgg caaacagcca
     5101 agccgcctgg atcgagcttg gcccggtcac tgtgagctaa ccgttctaga cctaccagca
     5161 tgcttcaggt ggctgagata tacagcgatc ctgtcctttg cttgacaata ccctacctag
     5221 taggtaaaat aggcgtgctc tgggaattga gctgtagatt caggccgaga tagctcagtt
     5281 ggtagagcac gcgactgaaa atcgcggtgt
//
LOCUS       DBNGSX010000383         2285 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1904208, whole genome shotgun sequence.
ACCESSION   DBNGSX010000383 DBNGSX010000000
VERSION     DBNGSX010000383.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1904208"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="AC16CB_11470"
     CDS             <1..237
                     /locus_tag="AC16CB_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3343133.1"
                     /translation="SLSNIFSDMWHAARQGVLFRSLGSIFWFRLMQFSGTYQGYRHHG
                     PLTWQLRQTFYYPRGIAPTGQAAARTVEPIRYNE"
     gene            244..924
                     /locus_tag="AC16CB_11475"
     CDS             244..924
                     /locus_tag="AC16CB_11475"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009785909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HIR3343134.1"
                     /translation="MTLVALVPMRHHSQRVPGKNYRPLGGKPLFHHIIESLLSCPEID
                     QVVVDTDSSPVMDGVRQNFPQVTLIERPEHLRADETPTNEVLLHDTAQVQADFYLQTH
                     STNPLLRPETISRAIQTFLSSYPAYDSLFSVTRWQTRLWDQLGRAINHNPAILLQTQD
                     LPPVYEENSCIYIFTRQNLVARRNRLGERPLLFEIGASEAWDIDEELDFAIVDFLMRQ
                     GIGDNGER"
     gene            983..1954
                     /locus_tag="AC16CB_11480"
     CDS             983..1954
                     /locus_tag="AC16CB_11480"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIR3343135.1"
                     /translation="MYTILLTAPYMVPLYERFLPVFKHYGLEVIVVPVKERLDEEQLL
                     SYAGKFDGALAGDDQYTARVLEACAPRLKVISKWGTGIDSIDQQACTHLGIKIGRTPN
                     AFTLPVSDSVMGYVLAFARRQPWMDRDIKAGVWHKLPGRSLSETTLGVVGLGNIGKAV
                     IRRARPFGMRLLGVDIIEIAPDFLVENGVEMTSLEDLLARADFVSLNCDLNPTSYHLM
                     DARTLTLMKPTAVLINTARGPVVDEPALIAALQSQSIAGAALDVYEHEPLPPESPLRQ
                     MENVLLAPHNSNSSPAAWERVHWNTLRNLLEGLGLDASSLESFKASG"
     gene            2020..>2285
                     /locus_tag="AC16CB_11485"
     CDS             2020..>2285
                     /locus_tag="AC16CB_11485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3343136.1"
                     /translation="MTQTPANPPPKAVLITGAAGGIGRACVNIFAQMGWRVLGVDRSA
                     FGPGFPSGSLFVQSDISVGENLKTIFNQAHAFTGYLNALVNNAAL"
BASE COUNT          466 a          735 c          599 g          485 t
ORIGIN      
        1 agcctgagca acatattcag cgatatgtgg cacgccgcac ggcaaggtgt cttgtttcgc
       61 agcctgggga gcatcttctg gtttcgcctg atgcagttct cgggcaccta tcagggctac
      121 cgccaccatg ggccgctcac ctggcagctc cggcagacat tttattatcc caggggtatt
      181 gcgccaactg gccaggcagc tgcgcggact gtggagccca ttcggtataa tgagtaataa
      241 tctatgaccc tcgtcgccct cgtccctatg cgccaccact cgcagcgcgt gccgggtaag
      301 aactaccgtc cgctgggggg caagccactg ttccatcaca tcatcgagag tcttctaagc
      361 tgcccagaga tcgatcaggt cgtggtggat actgacagct cgcccgtgat ggacggagtg
      421 cgccagaatt ttccccaggt gaccctcatc gagcgccccg agcacctgcg cgccgacgag
      481 acccccacca acgaagtgct gctgcacgac accgcccagg tgcaagccga tttttacctg
      541 cagacgcaca gcacaaaccc cctgctgcgc ccagagacga tctcgcgcgc catccagacc
      601 ttcctttcta gctatccggc ttacgattcg ctcttttccg tcacccgctg gcaaacccgc
      661 ttgtgggacc agcttgggcg ggcgatcaac cataacccgg cgatcctatt gcaaacacag
      721 gatctgcccc cggtttatga ggagaactcc tgcatataca tcttcactcg ccagaacctg
      781 gttgcccgcc gcaaccgcct gggagagcgc cccttgctct tcgagatcgg cgcttccgag
      841 gcctgggaca tcgacgagga gttggatttc gccatcgtag acttcttaat gagacagggg
      901 ataggcgata acggagagag gtaatccctc cgtttcccac cttcttatcc cctctcccct
      961 actcccttac ccctagtccc ttatgtacac catcctgctc actgctccct atatggtccc
     1021 tctgtatgaa cgtttcctcc cggtattcaa acattatggg ttggaggtga tcgttgtacc
     1081 agtgaaggaa cggctcgacg aggaacagct cctgtcctac gccggaaagt tcgacggtgc
     1141 cctggcaggc gatgaccagt atactgcccg cgtcctggag gcctgcgccc cgcggttgaa
     1201 agtgatctcc aaatggggca ccggtatcga ctctattgac cagcaagcct gcacccatct
     1261 agggatcaag attgggcgca ctcccaacgc attcacgctg cccgtctccg atagcgtgat
     1321 gggttatgtc ctagctttcg ctcgccgaca gccctggatg gaccgcgaca tcaaggccgg
     1381 ggtatggcat aaactccccg ggcgatcttt gagcgaaacc accctggggg tggtgggctt
     1441 aggcaatatc ggcaaggcgg tcatccgccg ggcgcgcccc ttcggcatgc gcctgctagg
     1501 ggttgacatc atcgagatcg caccagactt tctggtggag aacggcgttg agatgaccag
     1561 cctggaagac ctgctggctc gagccgattt cgtcagcttg aactgcgacc tgaaccccac
     1621 cagctaccac ctgatggatg cgcgcaccct gacattaatg aagcccacag ccgtgctgat
     1681 caacaccgct cgcggcccgg tagtagatga gcccgccctg atcgctgcct tgcaaagcca
     1741 atccatcgcc ggagctgccc tggacgtata cgagcatgag cccctcccgc ctgagagccc
     1801 gctcaggcag atggagaatg tcttgctggc gccgcataac tccaattcca gcccggcagc
     1861 ctgggagcgc gtccattgga acaccctccg caatctattg gaaggcttag ggctagacgc
     1921 cagttctctg gaatctttca aagcaagcgg ttgaaagtta acagttaacc gttatccccc
     1981 gaccgacaac cgcattacaa cctttaggtt taaagtgaca tgacacaaac ccctgctaac
     2041 ccacctccaa aggctgtctt aatcaccggc gcggctggtg gtatcgggcg cgcttgcgtg
     2101 aacatcttcg cccaaatggg ctggcgcgtg ctcggtgtgg atcgctccgc tttcggtccg
     2161 ggcttcccct ctggcagcct tttcgtccag tctgatattt ctgttggcga gaatttaaaa
     2221 actatcttca accaagcgca cgccttcact ggctacctga acgccctggt caacaacgcc
     2281 gccct
//
LOCUS       DBNGSX010000384        19974 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_587947, whole genome shotgun sequence.
ACCESSION   DBNGSX010000384 DBNGSX010000000
VERSION     DBNGSX010000384.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 19974)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19974)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19974
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_587947"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="AC16CB_11490"
     CDS             complement(<1..593)
                     /locus_tag="AC16CB_11490"
                     /inference="COORDINATES: protein motif:HMM:NF018012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR3343137.1"
                     /translation="MTPDRLPIEPIVSKHRIQFFSDEDLDQLQEATLQVLEKTGVQFP
                     TEKALSIFEKHGALVDRDTSIVKLPRDLVLKAMSTVPRYYRLGARDPALGFSLQEGYT
                     YMGVDGCGVETVDPKTGLTRSSTKADVGRTAKIVDYLSSTAFYWPMVGSQDFGKLSSL
                     HDLDASWNNTVKHIKTETLLGEVATRYAIEMATVVAG"
     gene            complement(639..3119)
                     /locus_tag="AC16CB_11495"
     CDS             complement(639..3119)
                     /locus_tag="AC16CB_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007227385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcvT family protein"
                     /protein_id="HIR3343138.1"
                     /translation="MTKSQDSNSVNTHIPTQARVVIIGGGVIGCSTAYHLTKLGWQDV
                     IVLERKQLTSGTTWHAAGLVVCGGFATDTMLYMAHYTRELYKQLENETGVSTGFKPVG
                     YIEIASSASRLEALRRNANFARGYGINVEEISPKEFKELWPLADARDVLAGFYTPEDG
                     RANPVDATLSLAKGARNQGARIYEETEVTGIKKKNGRVTGVVTNRGEIEAEFVVNCCG
                     MWARKVGQLAGVDVPLHAAEHYYLITEPMEGVSEDLPILEDVDRYAYYREEVGGLMLG
                     LFEPVAAPWGMGGIPQNFTFGEIQPDWDRMMPYIDKAMERIPAIKTAGIRKLFCGPES
                     FTPDLTLLLGEAPELRNFYVAAGLNSLGILLGGGVGKVLAQWIIDGLPPVDVADIHID
                     RMLPFQNTDQYLHDRTVEILGMMYKESFPNSQFVTARNARKSILHERLAKAGAYFASS
                     AGWEYPDWFAPPGVEPRVEYSWGRQNWFEYNAAEHKACRQDVILMDLSLMSKFLVQGR
                     DAEAILNRISTNDVSIPVGRIVYTEWLNERGTIEADLTVTRVAEDAFLVICSDLAHRH
                     VETWLKRHIPPEAHAFVTDVTSGYAMINVQGPKSRQLLSRVTNTDLSNEAFPYMTMKE
                     IDIHYARAMAYRITYLGELGYELYIPTEFAPSIFDLLVEAGQDLGLKHAGLQALNTLR
                     IEKAYRDYGHDIDVTDTPLEAGLSFVVDFNKPSGFIGREALLRQKESGILKNRFAQFL
                     LEDPEPLLYYGEQIYRDGKWVGYVRSGAYGFTLGGAVGLAFIENEEGVTADYIRSGKF
                     EIEVNKVRYPAKVSLRPMYDPKNERVKC"
     gene            complement(3408..4526)
                     /locus_tag="AC16CB_11500"
     CDS             complement(3408..4526)
                     /locus_tag="AC16CB_11500"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HIR3343139.1"
                     /translation="MSKKIRLVLLSIIILGSFSFGLVTSAKEAQKPVQYDLYFGDLHA
                     HTGYSDAWEGTPAEAFAAAKAAGADFMATTDHSYMLTADEWEQTLLAAAEYTSDSFVA
                     IPGYEFWMPGFGEVNVFNYADTPRSKPGLHGPGVKLPRWAVPGTFYDQLAARSGAVGL
                     WVHPTDYGDNFDDFAYWSDSRDQGMGLLEIHNYGSWIMPYALLEYEASYIMALDKGWH
                     VMPAANSDTHSPDWISGSEVRTVLLAQKLTHADLYAAMSASRGYATLDKNLRINYTLN
                     GAVMGSVLSSPASSYTASIHIQDPDGVPSDAITLVEVISDGGEVVASLPASSVTFDQK
                     ITLNSESAHYFYLRVTTASNVSGSEGVTAWTAPVWTGR"
     gene            complement(4757..5458)
                     /locus_tag="AC16CB_11505"
     CDS             complement(4757..5458)
                     /locus_tag="AC16CB_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343140.1"
                     /translation="MPILQDIEFSITADQILQAQGANPALIRKRKPNLVLTAERALDI
                     GLPLLEPLVLYRELELEKVRHETLILSEGYTLKSQLLTQHLAYAKIVVVILCSIGEKM
                     EKEIASLYDSDPVLGLALDGVGSAAVEALANATCKFFEARAQESKWQASIPLSPGMTS
                     WPVDEGQPEIFHILDPSSIDVRLTSSYLMLPRKSLTMVLGFGPQLMISGTTCSYCAMQ
                     ETCKYQDHYQASMLP"
     gene            5614..6117
                     /locus_tag="AC16CB_11510"
     CDS             5614..6117
                     /locus_tag="AC16CB_11510"
                     /inference="COORDINATES: protein motif:HMM:NF046293.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR3343141.1"
                     /translation="MLDDIDRSIISLLQYDGRTPHTKIAEELDINEGAVRRRVKKLID
                     EGIMQIVAIVEPHQLGWSEAAMIGITVNPNMIDEAATAISQLQEVTYLFQAAGEFDLF
                     AEVYCKNRAHFVSFLNNRLQKIPGIERTQTFLILKMYKLSYRWGETEAPLVKHTLTPQ
                     STEPTRK"
     gene            6131..7552
                     /locus_tag="AC16CB_11515"
     CDS             6131..7552
                     /locus_tag="AC16CB_11515"
                     /inference="COORDINATES: protein motif:HMM:NF018012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR3343142.1"
                     /translation="MNWNQSSDDFVELIHQKSAEILAEVGFCAPDKTMLSRLMAAGFP
                     VNEDTHMVRIPEELMKAALEALPKDVKLYYRGSQQAVDFGRGSLFMGAGTPVSVQDLK
                     TGDRRDATRSDVRNLVTIQDALPQVDIVRPTVTATDMGACSDLVEIAELLMHTEKPVV
                     HRTLSPDRVEAAVEMLAAVAGGYEALRAYPNFATLYCPISPGYFTPENIRCMLKWAEF
                     GIPITLLSMAMGGASAPATLLGELLVINTDILAWIVVLEILYPGTPLLYGSVSAVLDM
                     RTGILPLGAPERGMVNSGAAIMGHYYGIPSMCGGLSSDAKELDAQAGFEKAVTAVPLL
                     MEGASIIYGVGAVDAGSAISYSEMVMDDEFIGALRRMMQGIAIHDLDEEVELIKRLTP
                     RGNFLKEKHTRKNYHEHWQPHLLNREAYDTWAIDRKTVAQKARQRAENILQQHQPKPL
                     PVKAEAEIERILSRYLVVAEGSP"
     gene            7575..9026
                     /locus_tag="AC16CB_11520"
     CDS             7575..9026
                     /locus_tag="AC16CB_11520"
                     /inference="COORDINATES: protein motif:HMM:NF018012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR3343143.1"
                     /translation="MVEIQPIKSIYPFEALDADQLACIKDATLYVLDKVGVRFPSEKA
                     LRIFDEHGANVDFETKIVCMPPDLVLESMGKAPRNYTLSGRAEGTDLYLGNGYSYFST
                     DGCGHETLDFETGERRLSCKEDVAKMAWVCDYLSSIAFYWPIVSAQDFGQLAPLHEID
                     AAFNNTVKHVQTETVMGMGMAKYAIRMAEVVAGDMESLREAPVLSSLICTIAPLSQDR
                     EGIEGAMVFAEAGIPVGFMGMPNMGSTAPAVPGGALVVANAETVSAMVLMQLVAPGAP
                     VFQSILVSGMDPQSADYIVSSPEKYLCNVAAVQIAHDWGVPSLAGTFGVDCPEPASWQ
                     LGRDSVYTALLTALAGTDITIGLGMLKASTLLVPEQIIFDDEIYHIHRVLARGLDIRE
                     SSLAVDVIEAVGPAGHFLRQKHTRTNIRERWIPKLTHPSPSMDGTTTPHIRLRAKAEF
                     DRILKQHQPEPLSDRKQQELRTIIEIAQNDIGI"
     gene            9074..9724
                     /locus_tag="AC16CB_11525"
     CDS             9074..9724
                     /locus_tag="AC16CB_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HIR3343144.1"
                     /translation="MEDEMYKKLAQAVIDGEPEDAVALAKEALAQGLDPMECITQGLT
                     KGIQEVGTLFSCGDYFLPELIIGAEAMKDALAILEPALLADQKRDVVANVVLGTVEGD
                     VHEIGKTLVGTMLTANGFKVTDIGVDKPAAEFIEAIKETGATIVGVSALLTTTMLQQE
                     KIIRALEEAGLRNEVKVMVGGAPVTASYAKQIGADGYADDAISAVALAMRLADAPA"
     gene            9778..11595
                     /locus_tag="AC16CB_11530"
     CDS             9778..11595
                     /locus_tag="AC16CB_11530"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3343145.1"
                     /translation="MYLNTLKKNLIFGLLILLLAFSLASCGQKEAEVPTEVTVIEPTA
                     VVEPTEQPAEEPTVAVEPTAEEAPMPEQSTIVILVPEDPTGFNGLLTDTGYEQLVGEL
                     VMLSVAEVDPMGNVIPEIAAEIPTLENGGVEFDEENWTMNVIWKIRDDVYWADGEQLT
                     VDDIIFSWNVITDPDNGTWTDGLDYTDSIEKIDDFTFKVFYNTVYTAYQTQFGGENFF
                     VYPEHYCDASQGFFEWDCDKEPLSSGPFMLEEWVTNDHLTFVKNPNYFEAGKPGIDRV
                     IVQIIPEQTVRKTLMLEGDADLHVWPPDAIWQEYENAPDLQAVKSPTERWVMRFIPNL
                     AARGETDPVENPHPFLSDVRVRQAIRHAINVDTLIEDIFLGHGVPVWTEFFRPPYICD
                     IPRPEYSPQGAIDLLEEAGWTDSDGDGVRECRGCETAEEGTPMAMELAIYAEYGEELE
                     LAQQLVAEDLKAVGFDVTLQIVEGAVLWDPESGTEIQGNFDMDMWDDGYPGIDPTDNQ
                     LWYYYHTDASDPESGGWNVMRWQNEDFDALYDELFTLDEEYRTEVFCQIATLLDEEVP
                     QILLFSTLEMHGLSGHLNGVLPSVNDTVTWNIADWTLSE"
     gene            11689..12729
                     /locus_tag="AC16CB_11535"
     CDS             11689..12729
                     /locus_tag="AC16CB_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3343146.1"
                     /translation="MGKYIVRRLLQAIPILFLISVILFTLVNIAPGGPLGGHGRTRRV
                     KQEKIDALKRQFGLDKPLPLQYVIWLIGNDWMQVDEDGDGILDSYGLRKGILRGDFGI
                     SIKTRNPVLEEIGDRLPNTIYLMTVTLLVVAIVAIPIGIISALRQYSAFDITATTLSF
                     MGQAIPEFWLGLILILIFYAWLDNPFTGEPLLPSGGMQTIGMEFSLWDRIQHLILPVT
                     MGMVGWIAWYSRFLRSSMLEVINKDYIRTARAKGQSERKVLYKHALKNALIPLVTIIA
                     LDIPYIFSGSLYVELLFSWPGMGRLYYDAATFRDYPVLLAVLIIGASLIILSNLLADI
                     AYAFLDPRVRFE"
     gene            12838..13770
                     /locus_tag="AC16CB_11540"
     CDS             12838..13770
                     /locus_tag="AC16CB_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3343147.1"
                     /translation="MADVRDKKVKAQNSLSRHESMAAMIWRRFRHHPGAVVGAIVLSI
                     LIIATLLVPLSPYDPELSNVSMRLQPPSWQHPFGTDGLGRDMLTRVLYGGRISLLVGF
                     MVVVITLVIGVPIGAIAGYFGGWVDNILMRIIDATLALPSLLVLILLSAVLREVDLPL
                     IEKNNVLTIAVVIGLLSWMTVARLVRAIFLTLREIEYVTAAQALGASSLQIMASEILP
                     NGLGPIIVESTLEIGWAIMEESGLSFLGFGIQPPTASWGNLLGNAQEHLTTQPWLAIF
                     PGIMIFLTIISVNYIGDGLRDALDPYKILGQIGE"
     gene            13960..15348
                     /locus_tag="AC16CB_11545"
     CDS             13960..15348
                     /locus_tag="AC16CB_11545"
                     /inference="COORDINATES: protein motif:HMM:NF018012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR3343148.1"
                     /translation="MLSLQSALCEKDIHRIHEHSLDVLMEVGIAYNTPRALNILEERG
                     CQVDYSRNWACIPPDLVEWAISQAPRKVTLAARDPSRDVLLDGLKSHHTTDSQGTQAI
                     DLQTGILHDSNAEDLRNAALFADALNMIEIVNVSVSASDIPAHLRTLFHFSQVFTQTS
                     KHVRTGVLNAEQVPFLVEMAKIVTGSDAFRPIFSVIDCTISPLMHDGLMTEACIDLAK
                     LQVPILVYPMPLAGGTSPVTLGGTMLMHNVEFLSGLVLYQCVNPGTPVIYGTGSSQLD
                     MHTGRYGGSADGFGMGLSLINLATFYDLPANLWGLSTKSKELGTQYGYEATAGTMLSY
                     LAGADEIYSMGLLGSAQILSLEKMVLDNHLARQIEIMLAPIKVDEEHLQVDLIKRVGI
                     GGGFLNQRETLRYTREEYVPLWPPHGKDLKEMIHREALDILHNHQSPALPHGAREKIE
                     DILRHADKELGG"
     gene            15423..16886
                     /locus_tag="AC16CB_11550"
     CDS             15423..16886
                     /locus_tag="AC16CB_11550"
                     /inference="COORDINATES: protein motif:HMM:NF018012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR3343149.1"
                     /translation="MSVFSETPPLDPIVAKHHLRVLDDGQLSDLKKATLTILDQVGFH
                     CPSEKALVIYADHGGQVDFERQLVRLSPDVVEKAMSHAPRFYKMASRSAAHDIDLDGT
                     ATYCATDGCGVETIDFVTRERRNSKKDDVANMARVADYLSSIGFYWPIVSAQDYPEIA
                     PLHELHASFENTVKHVQTETVMDESMARYAVEMATVVVGDKEILRKRPVLSSLICTIS
                     PLAQDKGGLESALVFAEAGLPVGFMSMANVGSTGPATIAGTIVEADAEIIAALVLIQM
                     AFPGAPVYHSMMPGVMHPRTGAYMATTWEGSAIYAYGTEIAHHWGVPTLAGVFGTDGQ
                     VPGWQSAAEAASSLMMCALCKADTGAGLGLLEACTLLYPEAIVLDSDIYHRTRIEAGG
                     LDTSEEMLALEVIKQVGPRGHYLSHKHTRAHMRRRRFSDLVAQPTPDGGMREPVEVAR
                     ERVEYILNNHFPLPLEPAQQFELKHILQVAQKEFTQV"
     gene            16883..18316
                     /locus_tag="AC16CB_11555"
     CDS             16883..18316
                     /locus_tag="AC16CB_11555"
                     /inference="COORDINATES: protein motif:HMM:NF018012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR3343150.1"
                     /translation="MKTTIEVLDSFEKNRVHESTLDILANTGVMVNSALGRKYLAEAG
                     AEVDKNTKIVRFPRQLVEECIKLAPKHFKLGARRPGWDLEMNGDDCMLVADGEATYAV
                     DWPSTERRIATMEDWLIATRLLDSIDDIGVYWAMVESGLKDGSQFKLVKYWRHLLSNF
                     SKHLQDVINSKEQAPWILEILQIVFGDKQVIREQHPLSLLLCPQSPLMIDKQYTDAYL
                     SMTGWDIPVAIMPMPLMGGTGPANMISMVIQGNCEVLSMLCLVQAATPRTPVIYAPVL
                     AAMDPHTGLYSGGAIENGILGVAAIEMGRYYGLPVEGVGGGTSQFIPGIQAGYERVLT
                     GFMPTLKLPDLIVGPGLVGNSMVLSLEQLVIDVEIFRMSMHAYRGIDTREGNWLNDVI
                     QQVGPGGHYLSERSTVQAFRNGEMYLPNLGYRGPYLAWKEAGGTTLLQEAHEKVEYLL
                     ANYDPLPLSEEVERELELLQKRVEFTI"
     gene            18429..19739
                     /gene="gntE"
                     /locus_tag="AC16CB_11560"
     CDS             18429..19739
                     /gene="gntE"
                     /locus_tag="AC16CB_11560"
                     /inference="COORDINATES: protein motif:HMM:NF041362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanitoxin biosynthesis PLP-dependent
                     transaminase GntE"
                     /protein_id="HIR3343151.1"
                     /translation="MGLQGKNTKASYEKAKKLIPHGVNSNFRFWGDDDTLIVSHGEGA
                     YVWDLDGKRYIDYRLGFGPIILGHGYPAVVERVGEAIKGGVTFAWTTSLEIQVCERLT
                     RMCHVDKVRLTNTGSEATMHALRIARAYTGREKFIKFEGHYHGQCDYFMFSTASSSPA
                     ALGSRRAPIRAPVTSGIPTGINQYVIVLPFNDLERLEETIEAQWGDLAAVFVEPLMGN
                     CAGIVGKPEFIKKLRELCDKFGIMLVFDEVKTGFRIAKGGAQEFYDVRADLVTYAKAM
                     GNGFPIAAIAGKENVMMVIEPGAMAHGGTYSGNVVGTAAADATLEILENEPVFETIQQ
                     RGKALMKGIDDILAEAGLPHVVTGVPSMFGISLGSAKAPQEYRDYHKGDTELYEKISL
                     ELNRRGVQPDSDGREPWFLCYALSNEDVGETLNIFNDSVKAAMR"
BASE COUNT         4950 a         4633 c         5364 g         5027 t
ORIGIN      
        1 ctccctgcga caactgtagc catctcgatg gcatagcgag tggcgacctc tcccagcagc
       61 gtctcggttt taatgtgctt gaccgtattg ttccaggatg cgtccaaatc gtgcaaagag
      121 gataatttac cgaaatcttg cgagccgacc attggccagt aaaacgcggt ggaggataaa
      181 taatcgacga tcttcgccgt tctgcccaca tctgctttcg tagaggagcg ggtcagacct
      241 gttttgggat caacggtctc cactccgcat ccatccacac ccatataggt atatccttcc
      301 tggagagaaa aacccagcgc aggatcacgg gctcccaatc ggtagtaacg cggaacagtg
      361 gacatggctt tcaagaccag gtcccggggt agcttgacaa tgcttgtatc cctgtccacc
      421 agagcgccgt gcttctcaaa aatggacagg gctttttctg ttgggaattg cacacctgtt
      481 ttttcaagca cctggagggt tgcttcttgc aattggtcca gatcctcatc ggagaagaat
      541 tggatcctgt gcttggatac aattggctca atcggtaagc gatcaggggt cataatatat
      601 ctcctggaaa tcgatagggt tatttttaag ttagcagttc agcacttcac ccgttcgttc
      661 ttgggatcgt acatcgggcg cagtgagact ttcgcggggt agcgcacctt gttcacctcg
      721 atttcaaact tcccgctacg gatataatcg gcggttacac cttcttcatt ctctatgaaa
      781 gctaatccta cagctccacc aagcgtgaat ccataggcgc ctgaacggac atacccgacc
      841 catttgccat cccggtatat ttgttctccg tagtaaagga gaggctcggg atcctcgaga
      901 aggaattggg caaaccgatt ttttagaatt ccagattcct tttgtctgag cagcgcctcg
      961 cgcccaataa aaccactggg tttgttgaaa tcgaccacaa agctcaggcc tgcttccagc
     1021 ggggtgtcgg tcacatcgat atcatgacca taatcccggt aggctttttc gatcctgagc
     1081 gtgttcagcg cttgaagacc ggcgtgcttt aatcccaggt cttgtccggc ctcaactagc
     1141 aggtcaaaga tcgagggggc aaattcggtg gggatataca attcgtatcc aagctcgcca
     1201 agataagtaa ttcgataagc cattgcgcgc gcataatgga tatctatttc cttcatggtc
     1261 atatagggga aggcttcatt ggataggtct gtgtttgtca cacggctcag gagttggcgt
     1321 gattttggtc cctggacatt aatcatggcg tagcccgaag tgacatcggt gacgaaggca
     1381 tgcgcctctg gagggatatg ccgcttaagc caggtttcta catggcgatg ggccaggtcc
     1441 gagcagatca caaggaaggc gtcttctgcc acccgggtca cggtcagatc ggcttcaatt
     1501 gttccgcgtt catttagcca ctccgtgtaa acgatcctac ctaccgggat ggagacatca
     1561 ttggttgaaa tccggttgag gattgcttct gcatctctcc cttgaacgag gaatttggac
     1621 atcagtgaga gatccatcaa gatcacatct tgccggcaag ctttgtgttc ggcggcgttg
     1681 tactcaaacc aattctggcg tccccaagag tattcaaccc gtggctcaac acctgggggc
     1741 gcaaaccagt ccgggtattc ccacccggcg gaggaggcaa aatatgcgcc tgctttcgcc
     1801 aaacgctcat gcaggatgga tttgcgcgca ttcctggcag tcacaaactg agagttggga
     1861 aaactctcct tgtacatcat gcctaggatt tccacggtgc gatcgtgcag gtattggtca
     1921 gtattctgga agggcaacat cctgtcaatg tggatatcag cgacatctac cggcggtaaa
     1981 ccgtctatga tccattgcgc caggacctta cctacgccgc ctccgagcaa aatccccaag
     2041 gagtttagac cagctgccac gtaaaaattt ctcagttctg gcgcttcacc tagcaagagg
     2101 gttaaatcgg gggtgaaact ttctggaccg cagaatagtt ttctgatccc ggcagttttt
     2161 atcgctggaa tgcgctccat ggctttatca atgtaaggca tcattcgatc ccagtcaggt
     2221 tgaatctcgc caaaagtgaa attttgcggg attccaccca tgccccaggg cgcagccacc
     2281 ggctcaaaca gccctagcat caagccgccc acttcctccc ggtagtatgc ataacggtca
     2341 acatcttcta atatgggcag atcttcagag accccttcca tcggttcggt gatcaagtaa
     2401 taatgctcgg cagcatgcag gggtacatct accccagcca gttgaccgac ttttctggcc
     2461 cacatcccgc agcagttcac cacaaattcg gcttcaattt caccgcggtt cgtcacgaca
     2521 ccagtcaccc gcccattttt tttctttatc ccggtgacct ctgtttcttc gtagatgcgc
     2581 gcaccctgat tcctggcccc tttggctaga gataaagttg cgtctaccgg gtttgcacga
     2641 ccgtcttcgg gcgtgtagaa cccggcaagc acatcgcgtg cgtctgcaag aggccacaat
     2701 tccttgaact cctttgggga aatctcctct acgttgatgc cataaccccg agcgaaattt
     2761 gcatttcttc gcaatgcttc caagcgggac gccgaactgg caatttcaat gtaacccaca
     2821 ggcttaaatc cggttgatac cccggtttcg ttttctaatt gtttatatag ctcgcgggtg
     2881 tagtgcgcca tataaagcat tgtgtctgta gcaaaccccc cacagacgac caagccagcc
     2941 gcgtgccagg tggtgccaga tgttaactgc ttgcgttcaa ggacaatcac atcctgccag
     3001 cccagcttcg ttaggtggta agctgtactg cagccaatca ctccgccccc aataataaca
     3061 acccgcgcct gtgtagggat gtgtgtatta accgaattcg aatcctgcga tttagtcatg
     3121 attatccctc tgcatctcct ctcctgaaaa tttagctacg tgtgttaatc atttcaagtt
     3181 atgcatatgt attgacgagt atcccttccg acgagcaggc ctttttgtaa aggtcagcgt
     3241 atttgaacac agattgttaa agaccagcat ttgactcgcc aacttacgtc tcgtcggcct
     3301 agctttcaag tgaaaactgt gttagatccc tttgaatacc tggctgaaaa acgatccaac
     3361 tatttgcctg gcagaccggt attcttatgc tggatgccag ataaaattca gcgccctgtc
     3421 cacactggag ccgtccatgc tgtgacaccc tcactgccag aaacattaga tgctgtggtc
     3481 acacgcagat aaaagtagtg ggcggactcg gagttcaaag tgatcttctg gtcaaaggtc
     3541 acgctgctgg cgggcaggct ggcaacaacc tcacctccat cggagatgac ctccaccaaa
     3601 gtgatggcgt cgcttggaac cccgtctgga tcttggatat gaatgctggc ggtataggag
     3661 gaggcaggcg aggataaaac tgatcccatg actgccccgt tcagcgtgta gttgatacgc
     3721 aagttcttat ccagggttgc atagcctctg ctggcactca ttgctgcgta caggtcggcg
     3781 tgggttaatt tttgggccag cagcacagtc ctcacctcag agccagaaat ccagtctggc
     3841 gagtgcgtat cggaattggc tgccggcatc acatgccagc ccttatctag tgccataata
     3901 tagcttgctt cgtattccag aagcgcataa ggcatgatcc agcttccata gttgtggatc
     3961 tcaagcagcc ccatgccctg atcgcgggaa tcggaccagt aagcaaagtc gtcgaaatta
     4021 tcgccataat cggtggggtg aacccacaag ccgactgcgc ccgagcgggc ggcgagctga
     4081 tcatagaagg ttccgggaac cgcccaacgc ggcagtttca cgccagggcc atgcagaccg
     4141 ggttttgagc gcggcgtgtc ggcataatta aatacgttga cttctccgaa accaggcatc
     4201 caaaactcgt agcccggaat ggctacgaaa ctatctgaag tgtactccgc tgccgccagg
     4261 agcgtttgct cccattcgtc agcagtgagc atatagctgt ggtcggtggt tgccatgaaa
     4321 tcagcaccgg cagccttggc ggctgcaaag gcctctgctg gcgtgccttc ccacgcatcg
     4381 ctatagcctg tatgagcatg caggtccccg aagtacaaat catattggac tggtttttgt
     4441 gcttcctttg ctgaagtgac caaaccgaaa ctgaatgatc caaggataat gattgaaagg
     4501 agcactaatc ggattttttt actcatggct accctcccgg tttgaggatt cttgatgtgg
     4561 agttctttcg gccaatacga ataacaacaa acgattatgg gtttttgtga aaaggttggt
     4621 tcgattttta gtgaacctaa tgacttcgac tggatctgct gaacggactg aatgtcagtt
     4681 gcgatgaagt gaagctcaat aataattttc gctggttatt tccctagccg ggatggtgtg
     4741 atgcctggtg ctttgtctaa ggcagcattg aggcctgata atgatcttga tatttacaag
     4801 tttcctgcat agcgcagtag ctacaggtgg tgccactgat catcaactgg ggaccaaagc
     4861 ccaacaccat ggtgagcgat ttgcggggga gcataaggta agacgaggtg agccttacgt
     4921 caattgagct gggatccagg atgtgaaata tttctggctg accttcgtca acgggccagc
     4981 ttgtcatgcc gggacttagt ggaatcgacg cctgccactt gctttcttgg gcgcgggcct
     5041 caaaaaattt gcaggttgca ttggctagcg cttctaccgc tgctgaaccg actccgtcta
     5101 gtgccaaccc caagaccgga tcgctatcgt acaggctagc gatttccttt tccatttttt
     5161 cgccaatgct gcacaggatc acaacaacaa ttttggcata tgccaagtgt tgggtgagca
     5221 gctggctctt tagtgtatat ccctctgaaa gtatcaatgt ttcgtggcgg actttttcca
     5281 gctccaactc tcgatagagc acaaggggct cgagcaatgg aagaccgatg tcaagagccc
     5341 tttctgctgt caagacaaga ttgggtttgc gcttcctgat taacgccgga tttgcccctt
     5401 gggcctggag gatttgatca gcagtgattg agaattcaat atcttgcagg ataggcaagt
     5461 tctacagtct cctcaaagag tgtgtataaa ttatagcgaa caaaatgatg caagtcaatc
     5521 taaaaacggc gttattcgta aaatttgcta ataaaattta cgaataacga taaaatttat
     5581 tgactgtttt acgaattacg gtaaaatata agtatgttag atgatattga tagatccata
     5641 atttcgctcc tgcaatatga tggtcgcacc ccacatacta aaatcgccga ggagttggat
     5701 atcaacgagg gggccgttcg gcgtagggtg aaaaagctca tagatgaagg gatcatgcaa
     5761 attgtcgcca tcgttgagcc tcatcaattg ggttggagcg aggccgcaat gattgggata
     5821 actgttaatc cgaatatgat cgatgaggcc gccactgcaa ttagtcaatt gcaagaagta
     5881 acctatttgt ttcaagcagc aggcgaattt gatctgtttg cagaggtcta ttgcaagaat
     5941 cgagcgcact tcgtttcttt tctcaataac aggcttcaaa aaatccctgg cattgaacgg
     6001 acccagacat ttcttattct caaaatgtat aaattatcat atcgctgggg tgagacagag
     6061 gcgccgctcg ttaaacacac tcttacgcct caatccactg agccaacgcg caaataatta
     6121 agagacgatg atgaattgga atcaaagctc agatgatttt gtagaactta tccaccaaaa
     6181 atctgccgaa atccttgctg aggttggttt ctgcgcccct gataaaacaa tgctatcgcg
     6241 ccttatggca gctggtttcc ccgttaacga agatacccac atggttcgca tccctgagga
     6301 gttgatgaag gcggcacttg aggcgctccc caaagatgtg aagctctatt atcggggcag
     6361 tcaacaagcc gtagattttg gacgtggatc tttattcatg ggcgctggca cgccggtcag
     6421 cgtgcaggac ctaaaaaccg gggaccgccg tgatgccacc cgaagtgatg tgcgtaacct
     6481 cgtaactata caggatgctt tgccacaggt tgatatcgtc cggccaacgg taacggcaac
     6541 tgatatgggt gcgtgctccg atttggtaga aattgctgaa cttttgatgc atacagaaaa
     6601 gccggtggtc caccgtacgc tatcaccaga ccgcgttgaa gcggcggtag aaatgttggc
     6661 agcagtagct gggggatatg aggctttgcg ggcgtatcca aactttgcta ccttgtactg
     6721 tcccataagc ccgggttatt tcacgcccga gaatatccgc tgtatgctca aatgggctga
     6781 gtttgggata ccgattactt tgctgagcat ggcgatggga ggggcttctg ctccggctac
     6841 tcttttgggg gaactgctgg ttatcaatac agatatattg gcctggatcg tcgttctaga
     6901 gatcctttac cctggcacgc cgctattgta tggcagcgtt tcggctgtcc ttgatatgcg
     6961 gacaggtatt ttacccctag gcgcgccaga acgaggcatg gtcaacagtg gtgccgcgat
     7021 catggggcat tattatggta tcccttctat gtgcggaggc ttatcctcgg atgcaaaaga
     7081 attagacgca caagcaggtt ttgaaaaggc ggtcacggca gtgcctttgc ttatggaagg
     7141 cgcgagcatc atctacggtg taggcgcggt agatgccggt tcagcgatct cttacagcga
     7201 gatggttatg gatgacgagt ttatcggagc cctacgcagg atgatgcagg gtattgcgat
     7261 tcatgacctg gatgaagaag ttgagcttat caaacgtctc acaccaagag gtaatttcct
     7321 gaaagaaaaa cacacccgca aaaactacca tgaacactgg caaccgcatc tcttaaaccg
     7381 cgaagcctat gatacctggg caattgatcg caagacggta gcgcaaaaag ctcgtcaacg
     7441 agctgaaaac atcctgcaac aacatcaacc aaaaccattg cctgtaaaag ccgaagcaga
     7501 gattgaacgt atcctcagcc gttacttagt ggtagcggag ggttcgccgt gaactcccta
     7561 tataggagta actattggtt gagatccaac ccataaagtc gatatatccc ttcgaagcgc
     7621 ttgatgctga tcagcttgca tgcataaagg atgcaacgtt gtacgtgcta gacaaggttg
     7681 gtgtgcgttt tccatccgaa aaagccctga ggatatttga cgagcatggt gcgaatgtgg
     7741 attttgagac caagattgta tgcatgcccc cggatttggt tttggagtcg atgggcaaag
     7801 ctccccgaaa ttacaccctc tctggacgcg ccgaggggac agacctttat ctgggcaatg
     7861 gctactctta cttttcgacc gatggctgcg gacacgagac cctggatttc gagaccggcg
     7921 aaagacgtct atcgtgtaaa gaagatgtag ccaaaatggc atgggtctgc gattatcttt
     7981 cttcgattgc cttttattgg ccaatcgtca gcgctcaaga ttttggccag ctggcacctc
     8041 tgcatgagat cgatgcagct tttaataata cggtcaaaca tgtccagacc gaaacggtta
     8101 tgggcatggg aatggctaaa tacgcaatca ggatggcaga ggtggttgcc ggtgacatgg
     8161 aatccctgag agaggcccct gtgctttcca gccttatttg cacaattgct ccattgagcc
     8221 aggatcggga aggcattgaa ggggcaatgg tctttgctga agcagggata ccggttgggt
     8281 ttatggggat gcccaatatg ggatcaaccg cgccagctgt gccgggaggg gccttggtgg
     8341 tggcaaacgc cgaaacagtg agtgcgatgg ttcttatgca gctggtggca cctggcgccc
     8401 cggttttcca atccatcctg gtttccggta tggatccaca atcggcggat tatatcgtca
     8461 gctcgccgga gaagtatttg tgcaatgtgg cagctgtaca aatagcgcat gattggggtg
     8521 tgccatcgct ggcgggtacc tttggtgtgg actgcccaga accagctagc tggcaattag
     8581 gacgggatag cgtctacacc gctttgctga ctgcattggc tgggacggat ataacgattg
     8641 gtttaggaat gctgaaagca tctaccttgc tggttcctga gcagattatc tttgatgacg
     8701 agatttacca cattcaccgt gtgcttgccc ggggattaga tattcgggag tcgagcttgg
     8761 ctgttgatgt cattgaagct gtgggtccag ctggacattt cctgcgtcaa aaacacacgc
     8821 gaacgaatat tcgagaacgg tggataccca aattgaccca cccttctcca tcgatggatg
     8881 ggacaacaac gccgcatata cgcctgcggg ctaaggcaga attcgatcga atactaaagc
     8941 aacaccaacc cgagcccctt agcgatcgaa aacagcagga acttagaact atcatcgaga
     9001 tcgcgcaaaa tgacattgga atctgactta tgggggaaga tgacaaagac cgtatgagaa
     9061 aaaggaggaa tttatggaag acgaaatgta taaaaaacta gcccaagctg taattgatgg
     9121 cgaaccggaa gacgccgtag cgctcgccaa agaggcgcta gcgcaagggt tagaccccat
     9181 ggagtgcatc acgcaaggcc ttacaaaagg catacaagag gttggtacgc tattctcttg
     9241 cggcgattac tttttgccag agctgatcat cggtgccgag gcgatgaaag atgccttggc
     9301 aatcctcgag ccagctttgc ttgcggatca aaaacgcgat gtggtagcca atgtggtgct
     9361 cggtactgtt gaaggtgacg tacacgagat tggcaagaca ctggtgggga caatgttgac
     9421 tgccaatggc ttcaaggtca cagatattgg agttgacaaa ccagccgcag agtttatcga
     9481 ggccatcaag gaaacgggcg ccacaatcgt cggggtatcg gcattgctaa caaccacaat
     9541 gttacaacag gaaaaaataa tccgcgcctt ggaggaagca ggtctccgta atgaagtcaa
     9601 ggtcatggtc ggtggagcac ctgttaccgc gagttatgca aagcaaatcg gagccgatgg
     9661 ctatgctgat gatgcaatct cagcagtggc tctggctatg cgtctcgctg acgcgccagc
     9721 ataaatttgc tggaaatgtt ggttagattc gagcaacaat aacaaaagga ggcacacgtg
     9781 tatctgaaca ccctgaaaaa aaacctaatc ttcggtttgc tgattctcct cctggcattt
     9841 tctctggcct cgtgtggtca gaaagaagca gaagtgccga ctgaggtcac agtaatcgag
     9901 ccgacggctg tagtggaacc aactgagcaa ccagccgaag aaccaaccgt tgccgtggag
     9961 ccaactgctg aagaagcacc gatgcctgaa caatccacga ttgttatctt ggttccagaa
    10021 gacccaaccg gcttcaatgg tttgctgacc gatacaggtt atgagcagct ggtaggcgag
    10081 ctggtcatgc tgtcggtagc agaagtcgat cccatgggga acgttattcc agaaatagcc
    10141 gcggaaattc cgacactgga gaatgggggt gtggagttcg atgaagaaaa ctggacaatg
    10201 aatgtgatat ggaagatccg tgatgatgtc tactgggcgg atggtgagca gctcactgtt
    10261 gatgacatca tcttctcctg gaacgtcatc accgatccgg ataatggaac ctggacagat
    10321 ggtctggatt acaccgacag cattgagaag atagacgact tcaccttcaa ggtcttttac
    10381 aatacggttt acacagccta ccagacccag tttggcgggg aaaacttctt tgtatatcct
    10441 gagcattatt gcgatgcatc ccagggattt tttgagtggg attgtgacaa agaaccgcta
    10501 agcagcggtc cattcatgct ggaagagtgg gtgacaaacg atcacctgac ctttgtgaag
    10561 aaccccaact actttgaagc tggaaaacca gggattgaca gggttatcgt ccagatcatc
    10621 ccggagcaaa ctgtgcgaaa gacattgatg ctggaaggcg atgctgattt gcacgtttgg
    10681 ccaccagacg cgatttggca ggaatacgaa aacgcgcccg acctccaggc cgtgaagtcc
    10741 cccacggagc gatgggtgat gcgtttcatt ccgaacttag cagccagagg agagacagat
    10801 ccagtagaga atccacatcc attcctcagc gacgttcgcg tccgccaagc aattcgtcat
    10861 gcaattaacg tagataccct cattgaggac atattcctag gtcatggtgt gccggtgtgg
    10921 acggaatttt tccgcccgcc gtacatctgt gatatccctc gacccgaata ttctccacaa
    10981 ggggcaatag atttgcttga agaagccggg tggacagact ctgatggcga cggtgttcgt
    11041 gaatgccgtg gctgcgagac cgcagaagaa ggcaccccga tggcgatgga gctagcgata
    11101 tatgctgagt atggcgaaga gctagaattg gctcagcagt tggtcgcaga ggacctcaaa
    11161 gccgttggtt ttgatgtgac tttacagatc gttgagggtg cagttctgtg ggatcccgag
    11221 tcgggtaccg agatccaagg caatttcgac atggatatgt gggacgatgg atatccgggc
    11281 attgacccca cagacaacca gttgtggtac tactaccata cagatgcctc agatcctgag
    11341 agcggtggct ggaatgtcat gcgctggcaa aatgaggatt tcgacgcatt gtatgatgag
    11401 ctgtttactc tcgacgaaga atatcgcaca gaggttttct gtcagatagc cacacttctc
    11461 gatgaagagg tcccccaaat cctgctgttc tccacgctgg agatgcatgg gctttctggg
    11521 cacctaaacg gtgtgttgcc ttccgtcaac gacacggtga cttggaacat agcagactgg
    11581 acgctcagcg aataaccctc aagggtgatt ttggcaacac catctatcat agggagaagg
    11641 gcacatccct tctccctacc aatacttgaa aggactgaag gagtgtagat gggcaagtac
    11701 atcgttcgtc gcctacttca ggcaattcca atattatttc tcatcagtgt cattctgttt
    11761 accctcgtga acatcgcccc aggggggccc ctgggaggac atgggcgcac ccgacgggtc
    11821 aagcaggaaa agattgatgc gcttaagcga caatttggcc tggataaacc attgcctctt
    11881 caatatgtga tttggttgat tggtaacgat tggatgcagg tggacgaaga tggggatgga
    11941 atcttggaca gctatggctt aagaaagggc atcttgagag gagattttgg gatctcgata
    12001 aaaacgcgca atcctgtgtt agaagagatt ggagaccgat tacccaatac catttacttg
    12061 atgaccgtca ccctacttgt agtagccatt gtggcgattc ctattgggat catttccgcc
    12121 ctcaggcaat actcagcgtt tgatattact gctaccacat tgtcttttat ggggcaggcg
    12181 attccggagt tttggctggg cctgatcttg attctgatct tttacgcctg gttagataat
    12241 ccctttactg gagagccact tttaccgtca ggtggtatgc aaaccatcgg tatggaattt
    12301 tccctatggg atcgaattca gcatttgatc ttgccggtga cgatgggcat ggtcggttgg
    12361 atagcatggt actcgcggtt cttgcgctcg agtatgctcg aggtgatcaa caaagattat
    12421 attcggacag cgcgcgccaa aggacaatca gagcgaaagg tattgtacaa gcatgcgtta
    12481 aaaaacgccc taatccccct ggtgacgatt atcgccttgg atatccctta tattttttct
    12541 ggttcactgt acgtggaatt gcttttctca tggccaggca tggggcgtct ttactatgat
    12601 gcggctactt tccgcgatta tccggtgttg ctggcggttt taattatcgg tgcgagtcta
    12661 attatcttga gcaacttgtt ggcagatatt gcctatgcct ttttagaccc aagggttcgc
    12721 tttgagtgat ttctttttta ggaagctgct ggacggttta aaaatggatg acctcccaca
    12781 actcgcctag aacaaggcaa atcaaaaaga aacaatattt atcctgtgat gagtaacatg
    12841 gccgacgtaa gagacaaaaa ggtgaaagct cagaatagct tgtcaaggca tgaaagcatg
    12901 gctgccatga tctggcgaag gtttcgtcac catcctggcg cagtcgttgg ggctattgta
    12961 ctttccatat taatcattgc gacgcttcta gtaccgttat ctccatacga tcctgagctg
    13021 tccaatgtct caatgcgctt acaaccgccc tcgtggcagc atccttttgg gacggatggt
    13081 ttgggcaggg atatgctgac gcgcgtgctg tatggtggtc gcatatcatt gctggttgga
    13141 tttatggtgg ttgtcatcac gttagtaatc ggtgtcccca taggagcgat tgctgggtac
    13201 ttcggaggct gggtagacaa tatcttgatg cggattattg atgcaacgtt ggcattgcct
    13261 tctttgttgg tgcttatctt gcttagcgca gtattgaggg aagttgatct tccgctaatc
    13321 gaaaagaata acgtgttgac cattgccgtg gtgattggct tgctctcgtg gatgaccgtg
    13381 gcgcgtctag tacgggcgat ctttctcacc ctgcgggaaa tcgaatatgt cactgcagcg
    13441 caagccctag gcgcatctag cttgcagatt atggcaagtg agattctgcc aaatggctta
    13501 ggccctatta ttgttgaatc gacgctcgag attggttggg cgatcatgga agagtctggg
    13561 ctttcttttt tgggatttgg catccaaccc ccaacagcaa gttggggaaa tttgcttggc
    13621 aatgctcaag aacatctcac gacccaacct tggctggcaa ttttccccgg gataatgatc
    13681 tttctaacta ttatctctgt gaactacatt ggcgatggcc tgcgggatgc tttagatccc
    13741 tacaagatat taggacaaat tggtgaatga gaaaggaagc agaaaggaag gcggatggac
    13801 aatatcgcca tccagttgag gtagtgcgtg agcggattgt ttggcaagtg gatgctataa
    13861 gatcgggtca caagaaggtg gtaaacgtaa ggagatagct cgtagcctgg cttgtgccaa
    13921 taagagctga tcgaggaagg gtaattggag agatttcaaa tgttgtcttt gcagtcggcg
    13981 ttatgtgaga aagacattca tcggatccac gaacacagcc tggatgtgtt gatggaggtg
    14041 gggattgcat acaatacgcc ccgtgcactg aatatcttgg aggaaagggg ctgtcaggtg
    14101 gattacagcc gcaattgggc gtgcattcct cctgacctgg tcgaatgggc gatttcccaa
    14161 gcacctcgca aagtgacact cgcggcgcgt gacccatcca gggatgtgct gctggacggt
    14221 ttgaaatctc accacactac cgatagtcaa ggcactcaag cgattgacct gcaaaccgga
    14281 atacttcatg attccaatgc agaagatctg cgcaacgcag cgctttttgc cgacgctctg
    14341 aacatgatcg agattgtaaa tgtctctgtt tcagccagcg atattccggc ccacttgcgt
    14401 acgctttttc atttttctca agtatttaca caaaccagca aacacgtgcg cactggcgtg
    14461 ttgaacgccg aacaagtgcc tttcctggtg gaaatggcga agattgtaac cggcagcgat
    14521 gccttccgcc cgatattctc agtgattgat tgcaccatct cgcccttgat gcacgatgga
    14581 ctgatgaccg aggcatgcat cgatttagcc aaactgcaag ttcccatttt ggtttaccct
    14641 atgccgctgg cagggggtac ttccccagtg accttggggg gtacgatgct gatgcacaac
    14701 gtggagttcc tcagcgggtt ggtgttgtat caatgtgtaa accctggcac accggtgatc
    14761 tatggtacag gctcgtcgca attggatatg cacacaggca ggtatggtgg cagtgcggat
    14821 gggttcggaa tgggactttc gctgataaat cttgccacgt tctatgatct gccagcaaat
    14881 ctgtgggggc tttctactaa gtcaaaagaa cttggtactc aatatggcta cgaggcaacc
    14941 gcaggcacca tgctgtcata tttagcaggt gcagatgaaa tctacagtat gggactttta
    15001 gggtcggctc agatcttatc gttggaaaag atggttttgg ataatcattt agctcgccag
    15061 attgagatta tgctggcacc tatcaaggta gatgaagaac atctgcaggt cgatttgata
    15121 aagcgtgttg gaatcggcgg tggcttctta aaccagcggg aaaccctcag atatacccga
    15181 gaggagtatg tccctctttg gccaccacat ggcaaggatt tgaaagagat gatccatcgc
    15241 gaagccttgg atatcctgca caaccatcaa tctccggcac taccgcatgg tgcgcgcgag
    15301 aagatcgaag atattttgag acatgctgac aaagagttgg gcgggtgatg ttatcgaggt
    15361 ttaatgggca agggtttgac aaacctagcg tcaacacccc cacctgagac gagaggaatg
    15421 taatgtccgt ttttagtgaa actccgcctc ttgaccctat cgtagcgaag catcacttgc
    15481 gtgtcttaga cgatgggcag ctcagcgatc taaagaaggc tacattgacg attttagatc
    15541 aggtgggctt tcactgtcca tctgagaaag cgttagtgat ctacgcggac cacgggggtc
    15601 aggtggattt tgagagacag ctcgtgcgct tatcgccgga tgtggttgag aaagccatgt
    15661 cccatgcgcc tcgcttctac aagatggctt ctcgttctgc agcgcatgat attgaccttg
    15721 atggcactgc cacctactgt gccaccgatg ggtgtggggt tgagacgatc gattttgtga
    15781 cgcgcgaacg aaggaactcg aaaaaggacg atgtggccaa tatggccagg gtggcggatt
    15841 acctgtcttc aatcgggttc tactggccga ttgtctctgc gcaggattac cctgagatag
    15901 cacctttgca tgagctacat gcttcattcg aaaacaccgt taagcacgta caaactgaaa
    15961 ctgtgatgga tgagtctatg gcgcgctatg cggtcgaaat ggcaactgtg gtggtaggtg
    16021 acaaggaaat cttgcgtaaa cgaccggtcc tctcgtcttt gatatgcacg atctccccct
    16081 tggctcaaga taaggggggc ttggaatcag cgctggtgtt tgcagaagcc ggcctgccgg
    16141 tgggttttat gtctatggcg aatgtgggct caaccggtcc agccacaatt gctgggacga
    16201 ttgttgaggc tgatgcggag atcattgccg ccttggtgtt gatacagatg gcttttcctg
    16261 gagcacccgt ctatcactcg atgatgcctg gggttatgca tccacgtact ggggcatata
    16321 tggctactac ctgggagggg agcgcgattt acgcgtatgg tactgagatc gcccatcact
    16381 ggggagtgcc aaccctggcc ggtgtttttg gcacggatgg tcaggtgcct ggctggcaat
    16441 ctgcagctga agcagcttca tccttaatga tgtgcgcgtt atgcaaggcg gataccgggg
    16501 ctggattggg tttgctggaa gcatgcaccc tgctctatcc agaagcgatt gtcttagact
    16561 cggatattta tcaccggacg aggattgagg caggtgggtt agataccagt gaggagatgc
    16621 tggcattaga ggtcatcaag caggttggcc cccgcgggca ttatctcagc cataaacaca
    16681 ctcgtgccca tatgcgcaga cgaagatttt cagacctggt tgcccaacca accccggatg
    16741 gtggtatgcg agaacctgtt gaggtcgctc gtgagagggt ggagtatata ctcaacaatc
    16801 acttccccct gcctttggag ccagcccaac aatttgagct aaaacatatc ctgcaggttg
    16861 cgcagaagga atttacccaa gtatgaagac cacgatagaa gttctagata gttttgagaa
    16921 aaaccgggtg catgagagca ccttggacat actcgcaaac acgggcgtga tggtgaactc
    16981 tgccttggga cgaaaatatt tagcagaggc tggtgcagaa gttgataaaa acaccaaaat
    17041 agtacggttc ccacggcaat tggtcgagga gtgtataaag ttggctccga aacattttaa
    17101 gctgggagcc cgaagaccag gttgggatct tgagatgaat ggggatgatt gtatgctggt
    17161 ggctgatggt gaggctacct acgcagtcga ttggccatct acggagcgtc gtatagctac
    17221 catggaggac tggttgatcg caacccgctt gttagatagt attgatgata ttggtgtgta
    17281 ttgggcaatg gtggagagtg gcttgaaaga tggcagccaa tttaaattgg tgaaatactg
    17341 gaggcatcta ctaagtaatt tttcaaaaca cctccaagat gtcatcaata gcaaggaaca
    17401 agctccttgg atcttggaaa tactgcagat tgtcttcgga gataagcagg taatccgtga
    17461 gcaacatcca ctttcattat tgctatgccc gcaatcgccc ttaatgattg ataagcaata
    17521 taccgatgcg tatctgtcga tgacaggctg ggatattcca gttgctatca tgccaatgcc
    17581 cttgatgggt ggaacgggac ccgcgaatat gatctcaatg gtcatccagg ggaattgcga
    17641 agttctttcc atgctttgcc tggtccaggc agcgacaccg cgtacgccgg ttatctatgc
    17701 accggtactc gccgcgatgg atccccatac gggtttatat agtggagggg cgattgagaa
    17761 tggcattttg ggcgtggctg caattgagat gggtcgttat tatggtctgc cggtcgaagg
    17821 ggtaggggga gggacgtccc agttcatacc tgggattcag gcgggctatg aacgagtctt
    17881 gaccggattt atgcccacct tgaagttgcc agacttgatc gtaggaccgg gattggtcgg
    17941 caattctatg gtgttgagcc tggagcagct ggtcatagat gttgagattt tccggatgag
    18001 tatgcatgct tatcggggta ttgacacccg tgaaggaaat tggctcaatg atgtcattca
    18061 gcaggttggg cctggtgggc actatttgag cgaacgttca accgtccaag catttcggaa
    18121 tggtgagatg tacctgccaa atcttggcta tcgtggtcca tacctggcgt ggaaagaggc
    18181 cggtggaact acactgttac aagaagcgca tgaaaaagtt gaatacttgc ttgccaatta
    18241 tgatccatta cccctgtcgg aggaggtgga gcgcgaatta gagctgctac aaaagagggt
    18301 agagttcacc atttaaagtt atgttgagaa atggattggc agctgattat gacgaagatt
    18361 acctctcgct agcgatttct ggtgggaaga gtacatacaa actattattt taggtggagg
    18421 atatcaccat gggtctacag ggaaaaaata caaaggcttc gtatgagaaa gcaaagaaac
    18481 ttattccgca cggcgtaaac tcgaatttcc ggttctgggg agacgacgat acactaattg
    18541 tctcgcacgg ggagggtgca tatgtttggg atttagacgg caaaaggtac attgattacc
    18601 gattgggctt tggccccata attttggggc atgggtaccc ggctgtagtc gagcgggtgg
    18661 gagaggccat caaaggaggc gtgacatttg cctggacaac ctccctggaa atacaagtgt
    18721 gcgagcgcct cacgaggatg tgccatgtgg acaaggttcg cctgacaaat acgggttccg
    18781 aggctactat gcacgctttg agaattgctc gagcgtacac aggacgggaa aaattcataa
    18841 aatttgaggg ccactatcat ggtcaatgtg actattttat gtttagcaca gcttcttcct
    18901 ctccagcagc cctgggttcg cgccgggcgc ccatccgcgc gccggttacc tcaggtatcc
    18961 caacggggat taaccagtat gtgatcgttc tgccgttcaa tgatctggaa aggttggagg
    19021 agaccattga ggcccagtgg ggtgacctgg cggctgtctt tgtcgagcca ttgatgggca
    19081 attgtgctgg tattgttggg aagcccgagt tcatcaagaa gctccgcgag ctttgtgaca
    19141 aattcggtat catgcttgtt tttgacgagg taaaaactgg tttcaggatt gcaaaggggg
    19201 gcgcccaaga gttttatgac gtccgggcgg acctggtcac atatgccaag gccatgggaa
    19261 atggcttccc aattgcggca atagctggca aggaaaatgt catgatggtg atcgaacctg
    19321 gggcgatggc acatggcggc acttattctg gcaacgtagt tggcaccgca gcagcagatg
    19381 ccacactcga gatcttagag aatgagcctg tgttcgaaac catccagcaa cgtggcaaag
    19441 cactgatgaa aggcattgac gatatccttg ctgaagcagg gcttccccat gtcgtcactg
    19501 gggtgccctc tatgttcggg atatctctag ggagcgcaaa ggcgccgcaa gagtacagag
    19561 actaccataa aggggatact gagctttatg agaaaattag cctggagttg aaccggcgcg
    19621 gggtacaacc cgattctgat gggcgagagc cctggttcct gtgttacgcg ctgtccaatg
    19681 aagatgttgg tgagaccttg aatatattca acgactcagt aaaagctgcc atgcggtgat
    19741 ttctccggga attccccgga tggcagggtg attagaacga cgagtattga gcaatatttt
    19801 gaataaagga gcatcccatg cgtatcattg ttctgggtgg tgccggaaaa atgggttgta
    19861 tcgccgtcca gcatctggcg attgacccgc gcgttgatga agtcgtcatt gcggacatca
    19921 gccaagagaa tgcccggttg gtggcagaca ctatcaatag tccgaaaata gata
//
LOCUS       DBNGSX010000385         1742 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_588079, whole genome shotgun sequence.
ACCESSION   DBNGSX010000385 DBNGSX010000000
VERSION     DBNGSX010000385.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_588079"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="AC16CB_11565"
     CDS             complement(<1..1316)
                     /locus_tag="AC16CB_11565"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015087.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0070085 - obsolete glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HIR3343152.1"
                     /translation="MPHIKIGDQLIGDDQPTYIIAEIGVNHNGLLDLALKLIDIAKDA
                     GADAVKFQKRKLDTLYSKKYLDNANAGEKTLRYLLPILQQVELSEEAFYKILEHCQAQ
                     EITFMCSAFDAESADFLDGLGVPAYKVASADLTNLPLLDHLASKGKPLILSTGMSRME
                     EVELTVDFLQQRKAEFALLHCNSTYPAAFEDINLRFMDQLRRFGVPVGYSGHERGIAV
                     STVAAAMGASIIERHLTLDRTMDGPDHAASLEPQGFHKMVRDIRQVFQALGTGEEKFF
                     SRGEILNREVLGKSLVAARCIEPGEVIHAAMVAVKGPALGISPQRYPELVGRTAERVI
                     EEDEPFQESDLGHKILLDVEHTLPMEYGFTVRFRDFEELLAFKPRMLEYHFTDQDLDE
                     HYPGGDHNLKLVSHAPEFWDRTLVDLCSLDERQRCDSVKLMQKTIDL"
     gene            complement(1380..>1742)
                     /locus_tag="AC16CB_11570"
     CDS             complement(1380..>1742)
                     /locus_tag="AC16CB_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343153.1"
                     /translation="ADAVRTVCVPFQNPFKMWRIEPDGFMRPLGVELGISHEPYNQPR
                     QMLPEVYWQTGYVDVAWSDTILVKDSMTGERILPLIIDPSQWIDIDSADDWRRAERLL
                     ESGEITFEDLGYKIENRE"
BASE COUNT          349 a          487 c          525 g          381 t
ORIGIN      
        1 aggtcgatgg tcttctgcat cagcttcacg gagtcacagc gctggcgctc gtccagcgag
       61 cacaggtcga ccagcgtgcg atcccagaac tctggggcgt gtgataccag cttcagattg
      121 tgatcccctc cggggtagtg ctcgtctagg tcttggtcgg taaagtgata ttccagcatg
      181 cgcggcttga aagccaacag ctcttcgaaa tcacggaagc gcacggtgaa gccatactcc
      241 atcggcaagg tgtgttctac gtccaggagg atcttgtgac ccaagtcgct ctcttggaag
      301 ggctcgtcct cttcgatcac tcgttcggcg gtgcgtccca ccagttcagg gtagcgttgc
      361 ggcgagatgc ccagcgccgg tcccttcacc gccaccatgg ctgcatggat cacctcgccg
      421 ggctcgatgc accgggcggc gaccagactc ttgcccagca cctcgcggtt gaggatctca
      481 ccgcgcgaga agaatttctc ctcgccggtg cccaatgcct ggaagacctg gcggatatcg
      541 cgcaccatct tatggaaacc ttgtggctcc aggctggcag cgtgatcggg accatccatg
      601 gtgcggtcca gggtaagatg gcgttcgatg atgctggcgc ccatagccgc agccacagtg
      661 gagacagcga tgccgcgctc gtgcccggag tagccaacag gcacgccaaa gcggcgcaac
      721 tggtccatga agcgcaggtt gatatcctcg aaggcagccg gataggtact gttgcagtgc
      781 agcagggcga actccgcttt acgctgttgt aggaagtcca cggtgagctc cacctcctcc
      841 atgcgcgaca tacctgtgga gaggatgagc ggcttgccct tgctcgccag gtggtccagc
      901 aggggcaggt tggtcaggtc ggcagaggct actttatagg cgggcacacc caggccgtcc
      961 aggaagtcgg cgctctcggc gtcgaatgcc gagcacataa aggtgatctc ctgcgcctgg
     1021 caatgctcca ggattttgta gaaggcttct tccgagagct caacctgttg caagatgggc
     1081 agcaggtagc gcagtgtttt ctcgcctgca ttggcgttat ccagatattt ctttgaatag
     1141 agtgtatcca gcttgcgctt ctggaacttg accgcgtcgg cgccggcgtc cttggcgatg
     1201 tcaatcagct tcaaagccaa atcgagcagg ccattgtgat tgacgccaat ttccgcgatg
     1261 atgtaagtag gttgatcatc gccgatcaac tggtcgccga tcttaatatg gggcatgaaa
     1321 acctccagtt cgtcgagatt agggagtgga gagtaggaca caggttatac gagacgtgtc
     1381 tactctcgat tctcgatttt gtatcctaaa tcttcaaagg tgatttcgcc gctctccagc
     1441 agccgctcgg cgcggcgcca gtcgtcggcg gagtcgatat cgatccactg gctaggatcg
     1501 atgatcaagg gcaagatgcg ttctccggtc attgagtcct tgaccaggat ggtgtctgac
     1561 caggcgacat ccacatatcc ggtctgccag tagacttctg ggagcatctg gcggggctgg
     1621 ttgtatggct cgtgagagat gcccagctct acacccaagg ggcgcatgaa accatctggc
     1681 tcgatgcgcc acatcttgaa cggattttgg aatgggacgc acacggtacg cacggcgtcg
     1741 gc
//
LOCUS       DBNGSX010000386         2782 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_519345, whole genome shotgun sequence.
ACCESSION   DBNGSX010000386 DBNGSX010000000
VERSION     DBNGSX010000386.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_519345"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            318..980
                     /locus_tag="AC16CB_11575"
     CDS             318..980
                     /locus_tag="AC16CB_11575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015177776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HIR3343154.1"
                     /translation="MCGRFTLTADLSTLQEAFPFVHMPEGMQPRYNIAPSQPVAVVPN
                     DGRNRLDFFVWGLIPSWAKDPSIGNRMINARAETLQEKPSFRNAFRRRRCLVLADGFY
                     EWKKEGQGKIPTYIRLASGNPFAFAGLWESWNAPDGSNVLSCTIITTQPNEFMATIHN
                     RMPVILPESSYSLWLESGEQDTRRLADLLAPYTSGEMQAYPVSPLVNNPKHDVPELIQ
                     PR"
     gene            977..2335
                     /locus_tag="AC16CB_11580"
     CDS             977..2335
                     /locus_tag="AC16CB_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase type-2 family protein"
                     /protein_id="HIR3343155.1"
                     /translation="MTFSLTQTEWGEKVDRILVASLQAVEPASAIRRFVQRNADQLII
                     DGQIYDLASYRRIWVVGAGKAGALMACAMADILDERLDGGLVIVKDGYLQPCEGLIPE
                     RGLRLVEAGHPLPDGRGVRATQEIISLLSDCNAEDLVICLISGGGSALLTAPAPGISL
                     HDLQTLTQLLLASGANINEINALRKHLDQVKGGQLARMAYPAHLATLIVSDVVGNPLD
                     VIASGPTVSDGTTFSDAYAVLERYNLVGQVPQAILAYLQSGCAGEILETPKEGDSIFT
                     AVQNVIVGSNMQAVAAALRQAQQEGFNTQLLTTYMQGEARQVGRLLAAIVRQVATIGQ
                     PISRPACVVVGGETTVTLQGDGLGGRNQELALGAVEDLGGLMDVALVTLATDGGDGPT
                     DAAGAVVTGETLQRARQLGLRPSEYLQRNDAYHFFEALGDLIITGPTGTNVNDLAFLF
                     AF"
     gene            2558..2734
                     /locus_tag="AC16CB_11585"
     CDS             2558..2734
                     /locus_tag="AC16CB_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343156.1"
                     /translation="MVALLKFIFGLIGAVIGLVVGLIGLVVGLVGGVIGITVGCLVLA
                     IVLAPLILLLVLIF"
BASE COUNT          617 a          718 c          817 g          630 t
ORIGIN      
        1 tcagcgcctt gggcagcttc atgcaggtgc aggtgcgccc ggcaaaataa cttaaccgat
       61 ctctcagaac caggcacgta tcgataaggt caggggcaaa agcccctgac cttattcatg
      121 gtagggaaag cgttcagaac agcgctaaac ccattttcgc aaatatcaag gggacagggt
      181 gatgggcaca ataagagcat ctgaacggtt tcattggggt atacaatgac ccaaaattta
      241 tgtgagaatt ctttggtaaa atcgactcag ggattgtata agaataatca cttgatcccg
      301 caggaaggtg gtgttgtatg tgtggacgtt ttaccttgac ggctgacctg agcacgctgc
      361 aagaagcctt tcctttcgtg cacatgccag aaggcatgca gcctcgctat aacatcgccc
      421 ctagccaacc ggtagcggtg gtgcccaacg atggacgcaa tcggctggat tttttcgtct
      481 gggggttaat cccttcctgg gccaaggatc ccagcatcgg aaatcgcatg atcaacgccc
      541 gggctgagac gctccaggag aagccctcat tccgcaacgc cttccggcgg cggcgctgcc
      601 tggtgctcgc cgacggattc tacgagtgga agaaggaggg gcaaggcaag atccccacct
      661 acattcgcct ggcttcgggc aatccatttg ccttcgctgg attgtgggag tcatggaacg
      721 cgcccgacgg gtctaacgtg ctctcctgca cgattatcac cacccagcct aacgagttta
      781 tggcgaccat ccacaaccgc atgccggtca tactgcccga gagctcctac tcgctgtggc
      841 ttgaaagtgg agagcaagac acacgtcggc ttgccgatct gctggccccc tatacttcgg
      901 gcgagatgca ggcttatccg gtttcaccct tggtgaacaa cccaaaacat gacgtacccg
      961 aattaatcca gccgagatga cattctcttt gacacaaacc gagtggggcg aaaaggttga
     1021 tcgtatcctg gttgcctccc tgcaagctgt ggaacccgcc tcggcgatca ggcgctttgt
     1081 gcagcgcaac gcagaccaac ttatcatcga cggacagatt tacgacctag cgtcctaccg
     1141 tcggatttgg gtcgtggggg caggcaaggc tggggcgctg atggcttgcg ccatggctga
     1201 tatcttggat gagcgcctgg acggtggttt ggtaattgtc aaagatggct acctgcagcc
     1261 ttgcgaagga ttaatacctg agcgtggttt gcgtcttgtg gaggccgggc atccgctgcc
     1321 ggacggacgc ggggtacgcg cgacccagga aatcatctca ctgctgagcg actgcaatgc
     1381 tgaagacttg gtgatctgcc tgatctctgg gggcgggtcg gcgttgctca cagcaccagc
     1441 accaggcatt tcgttacatg acttgcaaac cctcacccaa ttactgctcg ccagcggtgc
     1501 caatatcaat gagatcaacg ccttgcgcaa gcatttggat caggtcaaag gggggcagct
     1561 ggctcgcatg gcgtacccgg ctcatctggc gaccttgatc gtctcagatg tggtgggcaa
     1621 cccgctggat gtgatcgcct ccggcccaac cgtatctgat ggcactactt tttcagatgc
     1681 ctacgcagtg ctggaacgct ataatctggt cggccaggtg ccgcaagcca tactggccta
     1741 tctgcaaagt gggtgcgcag gggaaatcct ggaaacgccc aaagaaggag attctatatt
     1801 caccgctgtt caaaatgtga tcgttgggag caatatgcag gctgtggcgg ctgccctcag
     1861 gcaggcccag caggagggct tcaacaccca gctgttgaca acctacatgc aaggggaagc
     1921 gcgacaggtg gggaggctgc tggcggcgat cgttcggcag gttgctacga taggccagcc
     1981 catctcgcgg cctgcatgtg tcgtggtagg tggtgaaacc acggtcaccc tgcaaggaga
     2041 cggcttgggg gggcgaaacc aggagctggc attaggagcc gtggaagacc tgggtggctt
     2101 aatggatgta gccctggtta cattggcaac tgatgggggt gacggcccaa ctgacgcggc
     2161 cggggcggtg gtcacggggg agacactcca gcgcgcccgc cagcttggtc tgcgtccttc
     2221 tgagtattta cagcgtaacg atgcctatca tttctttgaa gccctggggg atttgattat
     2281 caccggtccg accgggacga atgtgaacga tctggctttc ttgtttgcgt tttaaggatt
     2341 tgtaatacac atctgcaatg cttgggactg gaaacggcaa attgctctcc gattgcaact
     2401 aaaagctcaa aactgcgtag attgatataa atcaaagttt gcgcagttag atgaaaggcg
     2461 cgggaatcgc agcgagacac gctggcctaa tcccgccaaa gtcctcctag actgctcaac
     2521 tactgaaatc aatagaaagg aaatggaggt ccttgcgatg gtagcactat tgaaatttat
     2581 cttcgggctg attggagccg ttattggcct ggtggtgggg ctgatcgggt tagtggtcgg
     2641 cttggtaggt ggggtgatcg ggattacggt cgggtgcctg gttttggcga tcgtccttgc
     2701 gccactcatc ctgttgcttg tgctgatctt ctgatgccta tcatactgta tggctaaagc
     2761 ttgacacaac ccacttagag ct
//
LOCUS       DBNGSX010000387         1816 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1280943, whole genome shotgun sequence.
ACCESSION   DBNGSX010000387 DBNGSX010000000
VERSION     DBNGSX010000387.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1280943"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="AC16CB_11590"
     CDS             <1..936
                     /locus_tag="AC16CB_11590"
                     /inference="COORDINATES: protein motif:HMM:NF024491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR3343157.1"
                     /translation="VYFTDPSGPGAESLRGGPDSALAAAIDEARLSVDIAAYDFDLWS
                     LRDALMAAQRRGVTVRMVTDSDYIENDEVQELRDAGIQVLGDRREGLMHDKFVIIDRL
                     EVWGGSMNYTVNDAYKNNNNLIRIRSSRLADNYTTEFEEMFVDDQFGPRSPANTPYPS
                     LSVEGTTIEIYFSPDDGVADQLTGLIQSAQQSIHFLAYSYTSDDIADAMLERARAGIQ
                     VAGVFEENQVKSNRGDEYSRLRKAGLDVRLDGNPRNMHHKVILIDGQIVVTGSYNFSA
                     SAENSNDENTLVIFNSKIAAQFEDEFARVFAQAQP"
     gene            979..>1816
                     /locus_tag="AC16CB_11595"
     CDS             979..>1816
                     /locus_tag="AC16CB_11595"
                     /inference="COORDINATES: protein motif:HMM:NF014044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA domain-containing protein"
                     /protein_id="HIR3343158.1"
                     /translation="MDNSNFYLGRLFDTQHRKPTKSPLLYDSADLTTHAVVVGMTGSG
                     KTGLCIDLLEEAALQGIPALMIDPKGDITNTLLHFPDLLPQDFQPWVNADQARRGGKT
                     PEQAAEEASERWRQELNAWEIPAERVRALKNSAHFRVYTPGSDAGIPVSILASLKCPP
                     ISWDGNRELLREKISGTVTALLGLVGLDDIDPVRSREHILLSNIFEAAWSQAKDLDLG
                     ELILQTQTPPFAKLGVFDVNTFFPEKDRFELAMLLNNILAAPAFQIWIEGEPLEIQDL
                     LYT"
BASE COUNT          429 a          521 c          470 g          396 t
ORIGIN      
        1 gtctatttca cagatcccag cggtccaggc gccgaatcat tgcgcggtgg gccggattct
       61 gccctggctg ccgccattga cgaagctcgc ttgagcgtgg atatcgcagc ctatgatttc
      121 gacctgtgga gcctgcgtga tgcactcatg gctgcccagc gccgaggcgt caccgtgcgc
      181 atggtaaccg acagcgacta cattgaaaac gatgaagtcc aggagttaag ggatgcaggt
      241 atccaggtct tgggtgatcg ccgcgagggg ctgatgcacg acaaattcgt gatcatcgac
      301 cgcttggagg tgtggggcgg ctcaatgaat tacactgtca atgatgctta taagaataac
      361 aacaacctca tccgcatccg ctcatcgcgt ttggcagaca attacacaac cgaatttgaa
      421 gagatgttcg ttgacgacca gttcgggccg cgatcgccgg ccaacacccc ctacccaagc
      481 ttgagcgtcg agggaaccac aatcgaaatc tacttttcac ccgatgacgg cgtcgctgac
      541 caacttaccg gcctcattca aagtgctcag caaagcatcc acttcctggc atactcatat
      601 acatccgatg atatcgccga cgccatgcta gagcgcgccc gggcaggcat ccaggtggca
      661 ggcgtatttg aagagaatca ggtcaaatcc aatcgcggtg acgaatacag ccggttgcga
      721 aaagccggcc tggacgtgcg cctggatggt aaccctcgca acatgcacca taaagtcatt
      781 cttatcgacg ggcagatcgt tgtcaccgga tcatacaact tcagcgccag cgccgaaaac
      841 agcaatgacg aaaacacctt ggtgatcttt aattccaaaa ttgccgcaca atttgaggat
      901 gagtttgcaa gagtttttgc acaggcacag ccatagctgg atacccgttt agttcgtgtt
      961 cacatatctg ggatatttat ggacaactct aatttctacc ttggacgcct ctttgacaca
     1021 cagcatcgca agccgaccaa atcgcccctg ctctacgact cggcagacct gaccacacac
     1081 gctgtggttg tgggtatgac cgggtctggt aagaccggcc tgtgcattga cctgctggag
     1141 gaagccgcct tgcaaggcat ccctgccttg atgatcgatc ccaaaggcga catcaccaat
     1201 accctgctgc attttcccga tttactaccg caggatttcc agccctgggt gaatgccgac
     1261 caggcgcgtc gcgggggaaa aacccccgaa caggccgctg aggaagcgtc tgaacgctgg
     1321 cgacaggagc tgaatgcatg ggaaatccca gctgagcggg tacgcgccct gaaaaacagc
     1381 gcccacttcc gcgtttatac tcctggctcg gacgccggaa ttccggtcag cattctcgct
     1441 tcgctgaagt gcccgccgat ctcctgggac ggaaatcgcg aactcttacg ggagaagatc
     1501 tcaggcaccg tcactgcgct gctgggtttg gttggcttgg acgatatcga tccagtgcgc
     1561 tctcgggaac acatcctgct aagcaatatc tttgaggccg cctggagcca ggcaaaagat
     1621 ctcgacctag gcgaactcat cctgcaaact caaacacccc cctttgctaa acttggtgtt
     1681 ttcgatgtga acaccttctt cccagagaag gaccgttttg agctagccat gttgctcaac
     1741 aacatcttgg ccgctcctgc gttccagatt tggattgaag gtgagcccct ggagatccag
     1801 gaccttcttt acacct
//
LOCUS       DBNGSX010000388         3464 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1489389, whole genome shotgun sequence.
ACCESSION   DBNGSX010000388 DBNGSX010000000
VERSION     DBNGSX010000388.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1489389"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="AC16CB_11600"
     CDS             complement(<1..700)
                     /locus_tag="AC16CB_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3343159.1"
                     /translation="MEVFLVGLIAATLRVATPLIFGTLGELFSERAGILNLGIEGTMF
                     LGAFVGFAVAHTTGSLWLGFFAAVLAGILAGLLMGLLTVSLGLNQHVSGLGLTLFLTG
                     LSLFAYRLIFGERSLPPSISPFPKLSLFGNFPVLGPVFEQYALTYLAIALVLLFWWLI
                     YRTSFGLQLRAVGENPEAADAAGVNVFMMRYLALGFAGGLMAGGGAFLSLAQLGSFIF
                     GIIAGRGWVCIALII"
     gene            complement(700..1752)
                     /locus_tag="AC16CB_11605"
     CDS             complement(700..1752)
                     /locus_tag="AC16CB_11605"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3343160.1"
                     /translation="MPRLRFEKRAAPLWTRSLLPLMAVGVTFVLTSLLIFWAKANPFE
                     AYYYFLISPLSSRVSALEVLVKATPLLLTGAAVTFAFAAGYWNIGAEGQLLAGAIAAS
                     GLGIALENVSPFLAIPIMLVGGFLAGMLWASIPALLKVKLAVDEVVTTLLMNTVILFL
                     ISFLLNGIWRDPVSGWPQSPQIAEAATFPRLILRSRLHLGFLVAWIVVGFMWVVLYRT
                     PLGLRMRAVGLGQSAANFAGVNVGRTILIAALVSGGIAGLAGVSEVAGIHFHLIEAIS
                     PGYGYTGIIVATLGGLNPLGVSLAAIFIGLINTGAQSVSRALGVPIFLGNVVEAVLLL
                     VTLSMLILQNYRIKVA"
     gene            complement(1745..3241)
                     /locus_tag="AC16CB_11610"
     CDS             complement(1745..3241)
                     /locus_tag="AC16CB_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3343161.1"
                     /translation="MHAVELKGITKRFGEVIANRQIDFSLRQGEIQALLGENGAGKTT
                     LMRILYGLYTADEGQIIVNGQEVEIKSPRDAIRSGIGMVTQHFTLVPTLTVAENVVLG
                     YTRGFRLNMPELQNEVAKAASQFGIELATDALVRHLSVGERQRVEILKSLYRNARVLI
                     LDEPTAVLVPQEVEVLFETLQRLKQEGMSIVFISHKLYEVMAICDRITVLRDGQLVGT
                     VEKATTNQAELARMMVGRETFGVVRQGEYAQGGALQIENLSALDEKGLPALKNINLEL
                     KRGEILGIAGVSGNGQTELSQVLSGTRKPHSGKILLDGQDLAGATPAQFTALGVGRIP
                     EDRHSGIVAELDVAENLALENLDDFVNRGFLDRKTIHENAKRLIAEYQIKASPTDRIR
                     TLSGGNIQKVLLARALANNPQIVIAAQPTRGLDVGATEYVRTKLLEQRNAGAAVLLIS
                     EDLDEVITLSDRIAVIYEGRIMGVVPAQEADIEQLGLMMSGALEVDHA"
BASE COUNT          865 a         1059 c          826 g          714 t
ORIGIN      
        1 aaatgatcag cgcgatgcac acccagccgc gcccagcgat aatgccgaag atgaaggaac
       61 cgagctgcgc cagtgagagg aatgccccac cgcctgccat cagcccgccg gcgaaaccca
      121 gcgccaggta acgcatcata aatacattga cccccgccgc gtcggccgcc tcagggtttt
      181 cgcccactgc ccgtaattga aggccaaagc tggtcctgta aatcagccac cagaatagca
      241 gcaccagcgc gatcgccaag taggtgagcg catattgctc gaagaccggt cccaggactg
      301 gaaagttccc gaataaggag agtttcggaa atggggatat cgatgggggc aaacttcgct
      361 cgccaaaaat caggcggtag gcgaagagtg atagacccgt caaaaacagg gtaagcccca
      421 acccggagac gtgctggttc aagcccaggc taaccgtgag caaacccatg agtaaacccg
      481 ccaagatgcc agccagtact gccgcaaaga aacccaacca taaggaaccg gttgtatgag
      541 ccaccgcaaa gccgacaaac gcccccagga acatggtgcc ttcaatgccc aggttcaaaa
      601 ttccggcgcg ttcagagaat aattcaccca aagttccgaa aatcaatggc gtcgccacgc
      661 gcagtgtagc cgctatcaat ccgaccagaa aaacttccat cacgccacct ttatcctata
      721 attctgcagg ataagcatgc tcaaggttac cagtagcaga accgcttcga ccacattccc
      781 taggaaaatg ggcactccca gggcgcggct gacagactga gctcccgtgt tgatcaaacc
      841 gataaatatt gccgccaggc ttaccccgag cggattgagc ccacccagcg tagcgacgat
      901 aatgcctgta tatccatagc caggggagat ggcttcgatc agatggaaat gaatgcccgc
      961 cacctcgctc actcccgcca gacctgcaat accaccgctg accagggcag cgatcaaaat
     1021 ggtccgccca acgtttaccc cagcgaaatt agccgctgac tgcccgaggc ccactgctcg
     1081 catccgcagg ccgagcggcg tgcgatataa aacgacccac atgaacccaa ccacgatcca
     1141 ggccaccaga aaccccaggt gcagccttga acgcaaaatc aatcttggga acgtggcagc
     1201 ctccgcaatt tgaggcgact gcggccagcc agagactgga tcacgccaga ttccattgag
     1261 aagaaagcta atcaggaata agatgaccgt attcatcagc agggtggtca ccacctcatc
     1321 cactgccagt ttcactttga gcagcgcagg tattgacgcc cacagcatcc ctgccaaaaa
     1381 ccccccaacc agcatgatag ggattgccag gaagggtgag acattctcaa gcgctattcc
     1441 caggccagac gccgcaattg ctccagctaa aagctggccc tcagcgccga tattccagta
     1501 cccagccgca aacgcaaacg tgaccgccgc ccccgtcagc agcaaggggg tagccttgac
     1561 cagcacctct agcgcactca ccctactaga gagaggggag atgagaaaat agtaatacgc
     1621 ctcgaatggg ttagccttgg cccagaagat cagcagcgat gtcaggacaa aagtgacccc
     1681 caccgccatc aacggcagca gcgagcgcgt ccaaagcggg gctgccctct tttcaaacct
     1741 caacctaggc atgatccacc tccagcgcgc ccgacatcat caatcccaac tgctcgatgt
     1801 ccgcttcttg agcaggcacc actcccatga tcctgccctc gtaaatcacg gcgatgcggt
     1861 cggagagtgt gatcacctca tccaggtcct cagagatcaa caagaccgcc gcccccgcat
     1921 tgcgctgctc taacagtttg gtgcgcacgt attctgtagc tcccacatcc aacccgcggg
     1981 tgggttgcgc agcgattaca atctgcgggt tatttgccaa agcccgcgcc agcagcactt
     2041 tctggatgtt gcccccagag agggtccgga ttcgatctgt aggtgaagcc ttgatttggt
     2101 attccgcaat cagccgtttg gcattttcgt ggatggtttt tctatctaaa aatcctctat
     2161 tgacaaaatc atctaaattt tcaagagcca ggttctccgc cacgtccaat tccgccacga
     2221 tcccactatg tcggtcctct ggaatgcgcc ccaccccaag agcagtgaac tgtgccggag
     2281 tcgccccagc caagtcttgc ccgtcgagca aaatcttgcc gctatgaggt tttcgtgtgc
     2341 ccgagagaac ctgagataat tccgtctgac cattgccaga aaccccggca atccccaaga
     2401 tttcaccgcg ctttagctcc aggttgatgt ttttcagcgc cggtaagcct ttctcgtcta
     2461 atgcactcag gttttcaatt tgcaaagccc cgccttgtgc atattcaccc tgcctgacca
     2521 ccccaaatgt ttctcgaccc accatcatgc gcgccagctc agcctggttg gtggtcgcct
     2581 tttccaccgt tccaaccaat tgaccgtctc gtaatacagt gatccggtcg cagatcgcca
     2641 tcacttcata caatttatgg ctgataaaga cgatcgacat gccctcctgc ttcagccgtt
     2701 gcagagtttc gaacagtacc tcaacttctt gcggaacaag cactgcggtg ggttcatcca
     2761 agatcaacac gcgcgcattc cggtacaagg acttgagtat ctccaccctt tgtcgctcac
     2821 cgaccgagag gtgcctcacc agggcatctg ttgccagctc aatgccgaac tggctggcag
     2881 ccttggcaac ttcgttttgc agttctggca tattgagtcg aaaaccgcgc gtgtatccta
     2941 ggactacatt ctcagccacg gtgagcgttg gaaccagagt aaagtgttgc gtgaccatgc
     3001 cgatgccaga tcgaatggca tctctgggag attttatctc cacctcctgc ccattcacaa
     3061 tgatctggcc ctcgtcagcg gtatatagcc cataaaggat gcgcattagg gtcgttttcc
     3121 ccgcgccgtt ctcacccaac aaggcttgaa tctcgccctg gcgcaaggaa aaatcaatct
     3181 ggcggttggc gatcacctct ccaaatcgct tagtgatgcc ctttaattcc acagcatgca
     3241 taatgattcc tgttggtagg gctgtccaaa aagttgcgag ctttctaaac gtatctcgag
     3301 tctgtaactc gaatcctagc gctttttgga cagccccaga tgtaggtcag attatctaac
     3361 ccaagactga tcagataatc tgaccccctg tttgggtaaa ttttagttca ctggttcggg
     3421 ttgagcctcg tcgatatcaa ctcggaagag gccgctgatg atct
//
LOCUS       DBNGSX010000389         2613 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1142331, whole genome shotgun sequence.
ACCESSION   DBNGSX010000389 DBNGSX010000000
VERSION     DBNGSX010000389.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1142331"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="AC16CB_11615"
     CDS             complement(<1..344)
                     /locus_tag="AC16CB_11615"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3343162.1"
                     /translation="MITVKQILRSKGHAAWTISPDASVLEALQLMEQKDIGALVVVEG
                     KKLVGMFSERDYARKFIHGMGSTKDIQVRELMSAPVDTVRPGMSIGACMALMTERRFR
                     HLPVVEGENLVG"
     gene            complement(346..>2613)
                     /locus_tag="AC16CB_11620"
     CDS             complement(346..>2613)
                     /locus_tag="AC16CB_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3343163.1"
                     /translation="GKTPEQAAEEASERWRQELNAWEIPVERVRALKNSAHFRVYTPG
                     SDAGIPVSILASLKCPPISWDGNRELLREKISGTVTALLGLVGLDDIDPVRSREHILL
                     SNIFEAAWSQAKDLDLGELILQTQTPPFAKLGVFDVNTFFPEKDRFELAMLLNNILAA
                     PAFQIWIEGEPLEIQDLLYTSDGRPRHSVFYIAHLSDSERMFFVTLLYSAMETWMRSQ
                     PGTTSLRTILYFDEIFGYMPPVGNPPSKHPMLRMLKQGRAFGVGQVLVTQNPVDMDYK
                     ALSNAGTWFIGKLQTEQDKERLLDGLESAIASGLNRAEYDRLISTLGKRIFLLHNVHA
                     KGAQLFQTRWAMNFLAGPLTRAQIPQLNQLASGEPGLAVKDQQTQALMTDGVSDQTEI
                     NSVQAGPQPASTPTSPLVSSPAPVHSPQPMAASTRPTLPAGLKEYFLPYNLTFSQAFK
                     AAGRPLSKEAIIQGLVYRPVILAQVEVRFLNRKYNLDTILRKTALVAEPDRRGLVRWE
                     EYIAPGVDAASLDAAPDPQARYAVPEAPFTDPKTMTMLEKDFLDWVYRTIQVKVRANE
                     QLKLYAGPQISSAEFRTQCAEAARQGRDAEIRKISSAYDAKIATIQTKLSREERELSE
                     DQAELSQRKMEELGTHAENIFGIFGGRKSSRRLSSSLSKRRMTSKAKADVDESLDAIT
                     EYKKQIADLEKDKAAALQEVNDHWSQIANQTEEINITPLKKDILLDLFGVAWMPYHLI
                     RIGEELLELPAYAEI"
BASE COUNT          563 a          687 c          714 g          649 t
ORIGIN      
        1 atgccgacca aattttcacc ctcgacaaca ggcaggtggc ggaaacgccg ttcagtcatc
       61 agtgccatgc aagcgccgat actcatccct gggcggacgg tgtccaccgg cgcgctcatc
      121 agctcccgca cctggatatc tttggtcgaa cccattccat ggataaactt gcgggcatag
      181 tcgcgttctg agaacatgcc gacaagcttt ttgccttcaa caactaccaa cgcccctatg
      241 tctttctgtt ccatcaattg caaggcttca aggacgctgg catctggtga gatcgtccag
      301 gcagcatggc ccttagagcg cagaatctgt ttgactgtta tcatctcaaa tttcagcgta
      361 tgcaggtaat tcgagtaatt cttcgccaat acggattaga tggtacggca tccatgctac
      421 cccaaacagg tccagcaaga tatctttttt caggggagtg atgtttattt cttctgtttg
      481 attagcaatt tgactccagt gatcgttgac ctcctgcaag gcagcggctt tatctttctc
      541 cagatctgca atctgcttct tatattctgt gatggcgtcc agcgattcgt caacatcggc
      601 cttagctttt gacgtcatgc gtcgtttgga gagcgaactg gacagccgcc gggagctctt
      661 acgtccccca aatatgccaa aaatgttttc cgcatgggtg ccaagctctt ccatctttcg
      721 ctgtgagagc tcagcctgat cttcactcag ctcgcgctct tcgcgcgaca gttttgtctg
      781 tatggttgcg atcttcgcat cataggcgct ggagatcttg cgaatttcag catctcgacc
      841 ctgccgggcg gcttcggcac attgagtgcg gaactccgcg gaggaaatct gaggccccgc
      901 atagagtttg agttgttcgt tggcgcgcac ttttacttgg atggtgcgat agacccaatc
      961 taagaaatct ttttccagca tagtcattgt tttgggatcc gtgaagggcg cctctggcac
     1021 tgcatacctg gcttgcgggt cgggagcagc atccaggcta gcggcatcca ccccaggggc
     1081 tatgtactct tcccaacgaa ctagcccgcg ccggtctggc tctgccacta gggcggtctt
     1141 gcgcagtatg gtatccaagt tgtacttgcg attcaagaag cgcacttcca cctgagccag
     1201 gattaccggg cggtaaacta aaccctgaat gatggcctcc tttgatagcg ggcggccagc
     1261 ggctttgaaa gcttgtgaaa aggtcaggtt atagggtagg aagtattcct tcaacccggc
     1321 tggcagagtg ggacgagtcg aagcagccat gggttgaggt gagtggacag gcgcggggga
     1381 gctgacgagc ggagaggtgg gggtgctggc tggttgcggc ccagcttgga cggagtttat
     1441 ctccgtttga tcagacactc cgtctgtcat gagcgcctgc gtctgctgat ccttaacagc
     1501 taatcctggc tcgccactcg ccaattgatt taattgtggt atctgggcgc gagttagtgg
     1561 tccagccagg aagttcatcg cccagcgcgt ctggaataat tgtgcccctt tggcatgcac
     1621 gttgtgtaac aggaagatcc gcttgcccaa ggttgagatc aggcgatcgt attcggcgcg
     1681 gttgagaccg gaagcaatgg cactctccag tccgtctagc aagcgctcct tatcttgctc
     1741 agtttgcagt ttgccaataa accatgtccc ggcattcgag agagctttgt aatccatatc
     1801 tactggattt tgggtgacga gcacctgacc cacaccgaag gcgcgccctt gcttcagcat
     1861 acgcagcatg gggtgcttgg agggagggtt gcccactggt ggcatataac caaagatttc
     1921 gtcaaaatac aaaattgtgc gtaatgacgt cgtccctggc tgagaccgca tccaggtctc
     1981 catcgccgag tagagcagtg tgacaaaaaa catgcgttcc gaatcactaa gatgtgcaat
     2041 ataaaacaca ctatgacgcg gccgaccatc ggaggtgtaa agaaggtcct ggatctccag
     2101 gggctcacct tcaatccaaa tctggaacgc aggagcggcc aagatgttat tgagcaacat
     2161 ggctagctca aaacggtcct tctctgggaa gaaggtgttc acatcgaata cgcctagctt
     2221 ggcgaaaggt ggtgtttgag tttgcaggat gagttcgcct aagtctagat cttttgcctg
     2281 gctccaggcg gcctcgaaga tattgcttag caagatgtgt tcccgggagc gcactgggtc
     2341 gatatcgtcc aagcctacca aacccagcag cgcagtgacg gtgcctgaga tcttctcccg
     2401 taagagttcg cgatttccgt cccaggagat cggcgggcac ttcagcgaag cgagaatgct
     2461 gaccggaatt ccggcgtccg aaccaggagt ataaacgcgg aagtgggcgc tgtttttcag
     2521 ggcgcgtacc cgctcaactg ggatttccca tgcattcagc tcctgtcgcc agcgttcaga
     2581 cgcttcctca gcggcctgtt cgggggtttt tcc
//
LOCUS       DBNGSX010000390         1987 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1697556, whole genome shotgun sequence.
ACCESSION   DBNGSX010000390 DBNGSX010000000
VERSION     DBNGSX010000390.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1697556"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1171
                     /gene="moaA"
                     /locus_tag="AC16CB_11625"
     CDS             191..1171
                     /gene="moaA"
                     /locus_tag="AC16CB_11625"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIR3343164.1"
                     /translation="MALDRFGRSIHYLRISLTDHCNLRCVYCMPEDMTFRPNAELMQD
                     DELTLLVRLFASLGFDKMRLTGGEPTVRLHIVELVHAIARTPGVRSLSMTTNGILLSR
                     LARPLAEAGLQRVNISLDTLNPDKFRRVTRWGKLDDVWNGIMAAEEAGLTPVKLNAVI
                     VRGYNEPDIVDLARLTLERPWQVRFIEMMPFAGVTDLQTNNVVTAAETQATIEAALGS
                     LEVSNNGELDGEARLFRLPGAPGEIGFISSVSVPFCAACTRARLTADGRLRLCLLREG
                     EVDLLTPLRAGATLDDLRQLILKGIWHKPWGHGLAEGIIPLNRVMSEIGG"
     gene            1308..>1987
                     /locus_tag="AC16CB_11630"
     CDS             1308..>1987
                     /locus_tag="AC16CB_11630"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3343165.1"
                     /translation="MPSSHQIKAILFDLDGTLRHNRPSYTDVFFDISSRLGAPSVPEA
                     QYRAKRWMHYYWAKSDELLSDRRMYDDQTEAFYINHARLVLIAMGCTPDLAAALAPKA
                     YQMMKDEFKPQDWVPPDVHLTLDELKAAKYPLAVLSNRLRPFHEQMETLGLTPYFEFA
                     LHAGEAKSWKPDPQVFHQAVERLGYSPYETLYVGDNYFADVVGARRANLVPVLIDPEG
                     LFPEADCAV"
BASE COUNT          433 a          581 c          518 g          455 t
ORIGIN      
        1 cataagatcg aggagagggc gacaattagc gtaagaatgc caagggggcg tgttttcata
       61 ctttcaagcc tcttgctggc aagaattata aacaaaaatg cttggaaaac caatttgtgg
      121 acccaggggg gatcactaaa cactgaattt gtggtataac tttgataaaa ttagtctaaa
      181 ttaatctgct atggccctcg accgctttgg acgctccatc cactacttac gcatcagcct
      241 gactgatcac tgcaacctgc gctgcgtata ttgcatgcct gaagacatga ccttccggcc
      301 caatgccgag ctgatgcagg acgacgagct caccctgttg gtgcgcctct ttgccagcct
      361 gggcttcgac aagatgcgcc tgacaggcgg cgagccaact gtgcgccttc acatcgtcga
      421 actggtgcac gccatcgccc gcacgccggg ggtgcgctcg ctgtccatga ccaccaacgg
      481 gatcttgctt tcgcgcctgg cgcgccccct tgcggaggct ggcttgcagc gcgtgaatat
      541 cagcctggac acgctaaatc cggataaatt ccggcgtgtc actcgctggg gcaaactgga
      601 tgacgtatgg aatgggataa tggctgccga agaagcgggt ctcactccgg taaagctgaa
      661 tgctgtcatc gtgcgcggct acaacgagcc cgacatcgtt gatttagctc gcctcacgct
      721 tgagcgcccc tggcaagtgc gcttcatcga gatgatgccc ttcgcaggcg taaccgactt
      781 gcagactaac aacgtagtca ccgccgccga gacccaggca actattgaag ccgccctagg
      841 ctcgctggag gtatccaaca acggcgaact ggatggagaa gcgcgcctct tccgcctgcc
      901 gggagcccca ggtgaaattg gatttatctc atctgtcagc gtccctttct gcgctgcttg
      961 cacacgcgcc cgcctgaccg ccgacgggcg cctgcgcctg tgtttgttgc gcgagggcga
     1021 ggtagacctg ctgacaccgc tgcgtgcggg tgccacgctc gatgacctgc gccagcttat
     1081 cctgaaaggc atctggcaca aaccctgggg gcatggtctg gcagagggta taattcccct
     1141 gaaccgcgtg atgagtgaaa tcggggggta gacgaaggat aaaacgcaga gcggaagcta
     1201 atctcactca agctatatat cgccgaacat cttccgccgc ccacgatcct ccagtttttg
     1261 tctccgtttc tctctctgct tcataaacaa ctttaaggag gccttgtttg ccctcttctc
     1321 atcaaattaa agccatcctg ttcgacttgg atggaacgct acgccataac cgaccttcct
     1381 acacggatgt cttcttcgac atctcctccc gcttaggtgc gccgtctgta cctgaggccc
     1441 aatatcgcgc caaacgctgg atgcattatt attgggctaa atcagacgag ctgctctccg
     1501 accggcgcat gtatgacgac caaaccgaag ctttctatat aaaccatgcc cggctggtct
     1561 tgattgctat gggctgcacg cctgatcttg ctgctgcgct agcgccaaag gcctatcaga
     1621 tgatgaagga cgagttcaaa ccgcaagatt gggttccccc agatgtccac ctgactcttg
     1681 acgaacttaa agcagcaaaa tacccccttg ctgttctctc taaccgatta cgtccctttc
     1741 atgagcaaat ggaaaccttg ggactaactc cttacttcga gtttgctctc cacgctgggg
     1801 aggctaagtc ctggaaacca gatccacagg tcttccatca ggctgtcgag cgcctggggt
     1861 actcacccta tgagaccctg tatgtgggtg ataactactt cgcggatgtg gttggcgccc
     1921 ggcgcgccaa cctagtaccc gtgctgattg accccgaagg tttgttcccc gaggctgatt
     1981 gcgccgt
//
LOCUS       DBNGSX010000391         9489 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1627760, whole genome shotgun sequence.
ACCESSION   DBNGSX010000391 DBNGSX010000000
VERSION     DBNGSX010000391.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9489)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9489)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9489
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1627760"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="AC16CB_11635"
     CDS             complement(<1..350)
                     /locus_tag="AC16CB_11635"
                     /inference="COORDINATES: protein motif:HMM:NF024638.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3343166.1"
                     /translation="MQNDFGGQRVLILGAARQGLALARYLVQQGARVTLNDRTPADKM
                     ANAIASFKDISESVRWVMGSHPVELLNDADLVCVSGGVPLANPIVVEAQARGIPLSND
                     SQIFLEVTPCQVIG"
     gene            complement(378..1388)
                     /gene="mraY"
                     /locus_tag="AC16CB_11640"
     CDS             complement(378..1388)
                     /gene="mraY"
                     /locus_tag="AC16CB_11640"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR3343167.1"
                     /translation="MTQESAALALAVLSFALTVIWGTPLLRVLRYFKIGKSIRVEGPQ
                     RHFTKMGTPTMGGVLIVLPVLLITGLLNATTLLGLSVLGSSVLLPMAVMVSYALLGAL
                     DDWLGLRGRGEQGEGLRARTKFLIQLVLALAAALGLYYVLDVPNLYFPTVQGEIKLGF
                     WYVLIATFLIVSESNAVNLTDGLDGLAGLISATAFATFGAIALIQEQTFLARFCFTLV
                     GALFGFLWFNVHPAQLFMGDTGSLSLGATLAVVCLMTAQWPLLPIIAIIPFSNTLSVI
                     IQTMYFKFTRRRFGEGRRIFKMTPLHHHFELSGWSETQIVQRFWLISLLAGMIGVALA
                     VI"
     gene            complement(1385..2806)
                     /locus_tag="AC16CB_11645"
     CDS             complement(1385..2806)
                     /locus_tag="AC16CB_11645"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIR3343168.1"
                     /translation="MLTLADVLEALTGMRPAGGQQIVTDASIDSRQSIPGSMFIALPG
                     ERVDGHEFVGAAFERGAHLALIERDLSPQFKVLDLRVKLSQPDLDKFDGPFCLLVSNS
                     LQALQQVARYWRRKLNLRVIGITGSVGKSTTKEVVASVLEQRYRTLKNPGNMNNEIGL
                     PLSILDLGPGHEVAVFEMGFYVPGEIALLCDLALPQVGVITNIGTVHAERAGTQEAIA
                     RGKAELVQSLPAAPEGVAILNYDDPWVREMAGQTSAQVLFYGLDPQADLWAEEVESMG
                     LDGIRFRLHFQEESIYLRVPLIGRHSVHTVLRAAAVGLVEGLTWQEIVSGLRAGHAQL
                     RLVAVRTERGALLLDDTYNASPESTLAALNLLEDLEGRRVAVLGDMLELGLYEAHGHE
                     IVGVRAAEVVDELVTVGERGRMIAMAARSTGFDRNKITELENSQEAIEYLRPKLDEKD
                     VVLVKGSHGMRMDRIVAALEYRE"
     gene            complement(2808..4571)
                     /locus_tag="AC16CB_11650"
     CDS             complement(2808..4571)
                     /locus_tag="AC16CB_11650"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR3343169.1"
                     /translation="MMSEHVWRYTLLGTIFSLLAFAIVGQMVRIQLNPEQVAYFMEVR
                     EANLGTYRTIYPARGLIYDRDGHVMASNTKVYEVGVELAWVENPHTIALALNVVLGLD
                     YKDVLQAVSQDVSPSAVYVVLDKFVPQEKVDQLESYRDEIESNYTNRRDNDRPSLSGL
                     VYVPHLMRSYPEKDRASNILGFVTLEGEGNFGVEAKFNHLLAGDPKNVWIPRDPNLAE
                     EIPNIPDGASVILTIDRSIQRAVEDIVDDAVDDSGSDSGTIVVLAPQTGEILAMATTT
                     RLNLNEYWRYSEVFKGNTPFNRAVSEMYEPGSVFKILTMAAALDKGAVTPETEFLDTG
                     VFEIGGTYIYNWNRAAWGPQNMMGCLQHSLNVCLAWVSSELGAKDFYSYLRAFGIGHL
                     TGVDMAGEMPGRLKLPGDADWYDADLGTNAFGQGVAVTPLQLAVASAAIANDGKLMAP
                     HIVKAVVKDNQQFDTQPRLIGMPISSKTANTLTEMLASSLEKEASDALVEGYRVAGKT
                     GTAEIPTPYGYSSSATNASFVGWGPVDDPMFLVYIWLEKPTTSPWGSVVAAPVFQQVV
                     ERLVVLMEIPPDDVRRKIFSQ"
     gene            complement(4571..5047)
                     /locus_tag="AC16CB_11655"
     CDS             complement(4571..5047)
                     /locus_tag="AC16CB_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343170.1"
                     /translation="MDRVQSLTQAYSQAPWRKQTMFIGLFLLVVIFAALVAGIYLDVT
                     ARTATIGREIQGMEEDIETLKLANADLESKLALLMSTEEMEKRARSMGFKSIEMDEAM
                     YVVVPGYISRRHVQLAPPPGPVKTVAASLPTAFTESLFAWFQRKVVPMRSQILEVR"
     gene            complement(5152..6099)
                     /gene="rsmH"
                     /locus_tag="AC16CB_11660"
     CDS             complement(5152..6099)
                     /gene="rsmH"
                     /locus_tag="AC16CB_11660"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR3343171.1"
                     /translation="MHRSVLYQEIIHELRPRQNGYYVDATVGAGGHAWGILQASSPSG
                     RLLGLDVDPQALELARHRLQEFGERAVLVRASYIALNEQLADLGWAEVDGILLDLGMS
                     SMQVDTAERGFSFLVEAPLDMRFDPDNPVRAADLVNELPESELADIIYHYGEERRARQ
                     VARAIVAARPIHTTVQLAQVVSRVVRAASRSKDRQQHGIHPATRTFQALRIAVNRELD
                     ALEAVLPQCIKTLTTGGRLAVIAFHSLEDRIVKQFFRRESRDCICPPRQPVCTCGHKA
                     SLKEISRRPITPQSAEIEQNPRARSARLRVAEKLIGIEN"
     gene            complement(6126..6563)
                     /gene="mraZ"
                     /locus_tag="AC16CB_11665"
     CDS             complement(6126..6563)
                     /gene="mraZ"
                     /locus_tag="AC16CB_11665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR3343172.1"
                     /translation="MFMGQYVHSLDVKGRLTIPSRYRALLEDGAYITQGFDHNLMVWT
                     KPAFEQISHRVSQGSITDPTSRLLRRFIYSSGDAMEMDRAGRILIPEFLRQVAQLDSE
                     VVVVGVGDYFEIWSPSLWAEQLASMQDAEANQQRFMAFDLSAD"
     gene            6738..8177
                     /locus_tag="AC16CB_11670"
     CDS             6738..8177
                     /locus_tag="AC16CB_11670"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - obsolete cysteine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR3343173.1"
                     /translation="MTAPIIPTDHNYRDLFVGLDVNVPLLDGTTKRYINLDNAASTPS
                     FKFVQNLVDNFLSYYSSVHRGTGFKSQLSTHAYEEARHIVLRFLGADPATHICVFGKN
                     TTEAINKLARRFPFTPERDVVLISGMEHHSNDLPWRMRGAQNGQFDIKTIFIDLTPDG
                     QLDEVDFNNQLERYKDRLALVAVTGASNVTGYVNSIHRLAEKAHAAGALILVDCAQLA
                     PHRKVDMLSLDHPAHLDFVAISAHKMYAPFGTGALIGRRDIFEHGEPDMTGGGTVEIV
                     TKQDVVWAGPPDRDEAGSPNIVGAIALAAAIRQLEAVGMDAVGQHEADLTAYALEKLT
                     TVPGIAIFGDTRPAQTSTRLGVIPFELQGNSHFKAAAVLGYEFGIGVRSGCFCAHPYI
                     LHLLKLTPEETATARTEMLAGDKSNMPGLIRVSFGLYNTKDEVDSLVEALSSIQAGRY
                     HGNYTQNIATGEFTPEGWLPRFEEYFSFH"
     gene            complement(8189..>9489)
                     /locus_tag="AC16CB_11675"
     CDS             complement(8189..>9489)
                     /locus_tag="AC16CB_11675"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HIR3343174.1"
                     /translation="GYFALSHTNLNPLDEIIADIELPRNTPYNLDVTWPDIVASRVNH
                     FSFADLPLSLSGHISITLTNDHLPLLRVDGKTYELQPDENITEALSGYDPDWVWMAGS
                     LSTNDLVKGIGIGLLAAVLPALVYVSLLYWSDYYEKEPSRLLVSTFLWGALPALVLAI
                     IMRIFYRLPPGLFTPNATRALWVILLSPLTEEILKGVAVVYVAFRYRREFDNVLDGII
                     YGAMVGVGFAMTGNIISFIGSFLTRGFAGLGSTIFIEGVLYSLDNALYTAIFGAGMGF
                     YRLSQDRQRRYAVAIGAFALAVVTHGLHNTLIQNAVDMSPMIVLLSWSGLSVLVIAMA
                     WSLRRQRHCLELELIEELPGDMYHTIVQPRLRLQTEWQILKSEGFAAWKLRRRLFQLC
                     AEFAFKRMQARLFPDEAEIDAEANALRDEIRGILERYESQ"
BASE COUNT         2433 a         2677 c         2203 g         2176 t
ORIGIN      
        1 ataccgatca cctggcatgg agtcacctca agaaatattt gcgagtcatt cgagagcgga
       61 atacctctcg cctgtgcttc aacaacaatc gggttcgcca ggggtacgcc tcctgaaacg
      121 caaaccagat cggcatcatt aagtaactct accgggtggc ttcccataac ccagcgtaca
      181 gactcgctga tatccttaaa tgaagctatc gcattggcca ttttgtcagc cggtgtgcgg
      241 tcattaagtg tcacgcgcgc tccttgctga acaaggtaac gcgccaatgc caacccttgc
      301 cgggctgcac ccaatatcag cactctttga ccgccaaaat cgttttgcat cctgtctaac
      361 gttccgaaaa aacatattta aataacagcc aatgccacac cgatcattcc ggccagcagg
      421 ctgatcaacc agaaacgctg tacgatctgg gtttcgctcc atccagagag ctcaaaatgg
      481 tggtgtaaag gtgtcatctt gaagatacgc cttccttcgc caaatctacg ccgggtaaac
      541 ttaaaataca tggtctgaat aatcacactg agcgtattgc tgaacggtat gattgcgatg
      601 atgggcaaga gtggccattg agcagtcatc aaacacacca ccgccagtgt agctcccagt
      661 gatagtgagc cagtatcgcc catgaataac tgtgctgggt gcacattgaa ccacaagaag
      721 ccaaacaaag cgccaactag agtaaaacaa aaccgggcta gaaaggtttg ctcctggatc
      781 aatgctattg ccccgaaagt ggcaaatgcg gtagctgaga tcagtcctgc cagtccgtcc
      841 aagccatcgg tcaagttgac tgcgtttgac tcggacacaa tcaaaaatgt ggcgataagc
      901 acgtaccaaa atcccagctt gatctctccc tgtactgtag gaaaatacaa gttgggaacg
      961 tccaaaacat aatacaagcc gagtgcagct gccagggcta agacaagctg gatcaagaat
     1021 ttggttcgcg ctcgcaaacc ttctccctgt tctcctcgcc cgcgcagacc tagccaatca
     1081 tccaaagcgc ctaacagggc ataacttacc ataacggcca ttggcaatag cacagaacta
     1141 cccaaaacgg acaagccaag taatgtggtg gcattcaaca acccggtgat gagcagcact
     1201 ggcaggacaa ttagcacacc acccatggtt ggggtgccca tcttggtaaa gtgacgttgc
     1261 ggaccctcaa cgcggatgga tttaccgatc ttgaaataac gcaacacccg caataagggt
     1321 gtaccccaaa tcactgttag cgcaaaactg agcacagcca gcgctaatgc agcactttcc
     1381 tgggtcattc tctgtactcc agtgcagcca caatgcggtc catgcgcata ccgtgtgagc
     1441 ctttcactaa cacgacatcc ttctcatcga gtttcggtcg caagtattca atcgcttcct
     1501 gggaattttc aagctcggtg atcttattgc gatcaaatcc agtcgagcga gctgccatcg
     1561 caatcatgcg accacgctct cccacagtaa ccagctcatc caccacctct gcagcacgaa
     1621 caccaactat ctcatggcca tgagcctcat aaagccctaa ctcgagcatg tcgcccaaaa
     1681 ctgccactct gcgaccttca agatcttcaa gcaagttcag cgccgccagt gtcgattccg
     1741 gcgaagcgtt gtaggtgtca tccagaagca gagccccacg ttcggtgcgc acagctacca
     1801 agcgtaattg ggcgtgccct gcccttaatc cactcacaat ttcttgccat gtcaatcctt
     1861 caaccagacc tactgctgca gcccgtaaga ccgtatgcac tgaatggcga ccaatcaagg
     1921 gtactcgcag gtaaatcgac tcttcctgaa aatgtaaccg aaagcggatg ccatccaggc
     1981 ccatgctctc tacctcttca gcccacagat cagcttgggg gtccaggccg tagaagagaa
     2041 cttgggcact agtctgtcca gccatctcgc gcacccaagg gtcatcataa ttcagtatgg
     2101 ctacaccctc aggtgccgca ggcagcgact gaaccaactc ggcttttcct cgtgcgattg
     2161 cttcttgcgt gccagcccgc tcggcgtgaa ccgtgcctat attagtgatt acgcctacct
     2221 gtggcaacgc caggtcacat aagagagcaa tctcccctgg cacataaaaa cccatctcaa
     2281 aaactgccac ttcgtgtcct ggtcccagat ctagaatcga gagcggcaga ccgatctcgt
     2341 tgttcatatt tcctggattt tttaaggtgc gataccgctg ttccagtacg gacgccacca
     2401 cttctttggt ggtggatttt cctacgctgc cggtgatccc gatcacacgc aggttcaact
     2461 tccgccgcca gtagcgtgcc acttgttgta atgcctgcaa gctatttgag acgagcaagc
     2521 aaaacggccc atcaaactta tcaaggtctg gctggctcaa cttaactcgc aagtcgagca
     2581 ccttgaactg aggcgagagg tctcgctcta ttagcgctaa gtgcgcaccc ctttcaaatg
     2641 ctgcaccgac aaactcatgt ccatcaacac gctctccagg taatgctata aacatacttc
     2701 ctggtatgct ctggcgagag tcgatgctgg cgtccgtaac aatctgctga ccgccagcag
     2761 gtcgcatgcc tgtcaaggct tcaaggacat cagccagagt gagcatatca ttgcgaaaaa
     2821 atcttccgtc tcacatcatc tggtggtatc tccatcaaca caaccagacg ttctactacc
     2881 tgctgaaaaa caggtgccgc aactaccgat ccccaaggag aagtggtagg cttttctaac
     2941 cagatgtaca ccaagaacat cgggtcatcc accggacccc aacccacgaa tgaggcattc
     3001 gtagcactag aggaatagcc atagggtgtg ggaatttctg cagtgcctgt cttgcctgcg
     3061 acgcgataac cttccaccaa ggcgtcggag gcttcctttt ctaaggaact agccagcatt
     3121 tctgtaaggg tattcgccgt cttggacgag atgggcatac cgattagacg cggttgggta
     3181 tcgaattgct ggttatcttt tacaacagct tttacgatat gtggcgccat taatttgcca
     3241 tcgttggcga ttgccgccga tgcaaccgca agttgtaacg gtgttactgc cacaccttgg
     3301 ccaaaggcat tggtgcccag gtcggcatcg taccagtcgg catcgccggg taattttaac
     3361 cttccaggca tctcgccagc catgtctacc ccagttaagt gaccgattcc aaaagcccgc
     3421 agataagaat agaaatcctt agcacccaat tcggatgata cccaggctag gcacacattc
     3481 aacgaatgct gcaaacagcc catcatattc tgtggacccc atgcagcccg gttccagtta
     3541 tagatatacg tgccgccaat ttcaaagaca ccagtatcca aaaactctgt ctccggtgtc
     3601 acagcccctt tatccagagc agccgccatc gtcaagatct taaataccga gcctggctca
     3661 tacatctcgc taactgcgcg attaaacggc gtattgcctt taaacacctc ggaatagcgc
     3721 cagtattcat ttaggttcaa ccgggtagtc gtcgccatag ccagaatctc gccggtttgt
     3781 ggggcaagca caacaatggt gcccgaatcg gagccgctat catccacggc atcgtcaacg
     3841 atatcttcca cagctctttg aatgctgcga tcgatggtca gaatcacgct cgccccatct
     3901 gggatattgg gtatctcttc agctaggttt ggatcccggg gaatccagac attcttcgga
     3961 tctcctgcca gcaaatggtt gaacttggcc tccacgccaa aatttccctc cccctccaag
     4021 gtcacaaagc ccagtatatt agaagctcga tctttctccg ggtagctgcg cattaaatgc
     4081 ggaacataca ccaatccaga caagctcggg cgatcattat cccggcgatt cgtataattg
     4141 ctttcgatct catcgcgata gctctctagc tgatcaacct tctcctgggg cacaaatttg
     4201 tctaggacaa catagacagc gcttggggag acatcctggc tgacagcctg taagacgtct
     4261 ttatagtcca atccgagaac gacattgagc gccagagcaa tcgtgtgtgg gttctcaacc
     4321 caggccagct caaccccgac ttcatagacc ttggtattgc ttgccatcac atgcccgtct
     4381 cgatcgtaga tcaacccgcg tgcaggatat atcgtacggt aggtgcctag gtttgcttcc
     4441 cgcacctcca taaaatatgc cacctgttct gggttaagct ggatacgcac catctggccg
     4501 acaatagcaa acgccaagag ggagaaaatc gtccccagca aggtataccg ccagacatgc
     4561 tcgctcatca tcaccgcacc tccaaaatct ggctgcgcat tggcacaacc tttctttgga
     4621 accatgcaaa cagcgattct gtgaaggcag ttggcagact tgcagccacc gttttcaccg
     4681 gacccggagg tggggccagc tggacgtgtc gccttgaaat gtagccagga actaccacat
     4741 acatggcctc gtccatctcg atggacttga agcccatact gcgggcgcgc ttttccattt
     4801 cttctgtgga catcaacagg gcaagcttgg actccaggtc ggcatttgcc aatttcagag
     4861 tttcgatatc ttcttccatc ccctgtatct ctcgaccaat ggtggctgta cgcgcggtta
     4921 catctaagta aatgccagca accagcgcag caaaaatcac gaccagtaaa aataagccaa
     4981 taaacatggt ctgcttacgc cagggggctt gcgagtatgc ctgagttaaa ctttgaacgc
     5041 ggtccattcc atcctcctgc aggatttggc tggatttctc cggtacccaa caagcttgca
     5101 agtgtcatgc caaaaagtaa cgccgttcaa gatatttacc ttacaactaa attaattctc
     5161 aatgccaatc agtttttctg ctacgcgcag ccgggcactg cgagcgcgcg ggttttgttc
     5221 aatttccgct gactgaggcg tgatcggtcg gcgggagatc tctttcagcg aagccttatg
     5281 cccgcatgta cacactggct gtcgtggtgg acagatacaa tcccggcttt cgcggcgaaa
     5341 gaactgcttc actatgcgat cttccagcga gtggaaggcg attaccgcta gccgaccacc
     5401 cgtcgttaag gttttaatac actgtggcaa gacagcctcc agagcatcca actcgcggtt
     5461 tacagcaatg cgcagtgcct ggaaggttcg tgttgccgga tggatgccat gctgctgcct
     5521 gtccttgctc ctgctggctg cccttaccac tctagagacc acctgtgcca gttgcactgt
     5581 ggtgtggatc ggccgtgctg ctacgatggc gcgtgccacc tggcgtgcac gccgttcctc
     5641 gccatagtgg taaatgatat ccgccagctc agactcaggt aattcattga ccaggtctgc
     5701 tgccctgact gggttatcgg ggtcgaaacg catgtctagg ggagcctcca ccagaaagga
     5761 aaatcctcgc tcggcagtat caacctgcat ggacgacatg cccaggtcta ataagatgcc
     5821 atccacttcc gcccagccaa ggtcggcaag ttgttcattt agggcgatgt aagatgccct
     5881 aaccagaacc gctcgctcgc cgaattcctg caagcgatgg cgggcaagct caagtgcttg
     5941 gggatccaca tccaggccca gcaatcgtcc gctgggcgaa ctggcttgca gaatacccca
     6001 tgcgtggccc ccagctccca ccgtggcatc tacatagtac ccattctgcc ttggtcgtaa
     6061 ctcgtgtata atctcttggt aaagaactga acggtgcatg gatatctcac tcggatatcc
     6121 tggtgctaat ccgctgataa atcaaacgcc atgaaacgct gttggtttgc ctcagcatct
     6181 tgcatgctgg caagctgctc agcccacagc gaaggagacc agatttcaaa ataatccccc
     6241 acccccacaa ccacaacctc gctatccagt tgagctacct gacgcagaaa ctccgggata
     6301 aggatgcgcc ctgcccgatc catttccatt gcatccccac ttgagtagat gaaccgccgc
     6361 aggaggcggg atgttgggtc ggtgatgctg ccttggctga cacggtgaga gatctgctca
     6421 aaagcaggct tggtccatac cattaaattg tgatcaaacc cttgggtaat atatgcacca
     6481 tcttccagca aagcccgata gcgagaaggg atcgtcaatc gacctttgac atccaagcta
     6541 tgtacatact gacccatgaa catatgttct aacctctcgt taacggttga acgccggaaa
     6601 ataccggcgc tccactttct cccactttct accacatata gacacagtat atacgaaaat
     6661 tgtccgaatt gcaagccctt tttcccacca atttaggtca atatattttc ccaacagatt
     6721 tgtcagaaag gtggaccatg actgccccta tcatccccac agaccacaat taccgcgatc
     6781 tatttgtcgg cctggatgtc aatgtcccgc ttttagacgg cacaacgaaa cgttacatta
     6841 acctggataa cgccgcatct acaccctcat tcaagtttgt gcagaatctt gtggataact
     6901 ttctgagcta ctatagcagc gtgcatcgcg gtaccggttt caaaagccag ctttcgacgc
     6961 acgcctacga ggaagcccgt cacatcgtgc tccgcttcct gggcgccgat ccagcgaccc
     7021 atatttgtgt atttggcaag aacacaaccg aggccatcaa caaactagcg cggcgtttcc
     7081 cttttacccc agaacgggac gtggtgctta tcagcggaat ggaacatcat tctaacgact
     7141 taccctggcg catgcgcggc gcacagaacg ggcagttcga cataaaaact atcttcatag
     7201 atctcacccc agatgggcaa ctggatgagg tcgattttaa taaccaatta gagcgatata
     7261 aagatcgcct ggcattggtc gctgtaactg gtgccagcaa tgtgaccggc tacgtcaatt
     7321 ccattcaccg cctggcggaa aaagctcacg ccgcaggcgc actgatcctg gtggactgcg
     7381 cacaacttgc tccacatcga aaagtagata tgctgtccct cgatcacccg gcccaccttg
     7441 acttcgtggc aatctcagcc cacaaaatgt acgcaccctt tggcactggg gcgctgatag
     7501 gcaggcggga tattttcgag catggtgagc cggatatgac cggcggtggt acggtggaga
     7561 tcgtcactaa gcaagatgtg gtctgggcag gccctcctga ccgcgacgaa gcaggtagtc
     7621 caaacattgt cggcgcaatc gccctggcgg ctgccattcg ccagctcgaa gcggtgggta
     7681 tggacgccgt aggacaacac gaagccgatc tgactgccta tgctttggag aagctgacca
     7741 ctgttccagg cattgccata tttggtgata ctcgccccgc ccaaacatca actcgcctgg
     7801 gagtgatccc ctttgagttg caaggcaatt cccactttaa agctgctgcg gttctcggat
     7861 atgaattcgg catcggcgtg cgcagcggct gcttctgcgc acacccctac atccttcact
     7921 tgttgaaatt aacacctgaa gaaacagcta ctgcacgaac tgaaatgctg gcgggggata
     7981 agtcaaacat gcctggctta atccgcgttt cttttggtct gtataacaca aaagacgaag
     8041 tggattcgct agtcgaagca ttgagcagca tccaggcagg acgctaccat ggcaactaca
     8101 cgcaaaatat tgccacgggt gaatttacgc ctgaggggtg gctcccaagg tttgaagagt
     8161 atttctcttt ccattaatag aatacaattt attgagactc gtacctttct aatatccccc
     8221 ttatttcgtc ccgcaatgca tttgcttcgg cgtcgatctc cgcctcatcc gggaataagc
     8281 gcgcttgcat acgcttgaaa gcgaattctg cacagagttg gaagagccgc cgcctcagct
     8341 tccaagcagc aaatccttcg cttttcaaaa tttgccattc ggtctgtaat cttagcctgg
     8401 gctgcacgat cgtatgatac atgtcccctg gcaattcttc gatcagctct aattccaggc
     8461 aatggcgctg ccgtctcaat gaccacgcca tcgcaatcac gagaacggat aacccgctcc
     8521 aggacagcaa gacaatcatc gggctcatat ctaccgcgtt ttgaattaat gtgttgtgca
     8581 atccgtgggt gacaaccgcc agagcaaaag ccccaatagc cacagcgtaa cgcctttgac
     8641 ggtcttgaga caggcgataa aagcccatcc ccgcaccaaa gatggcagta tagagtgcat
     8701 tgtctaaact gtaaagcacg ccttctatga aaatcgtgct acccaaacca gcaaatccgc
     8761 gcgtcaaaaa agacccgatg aaactgatga tatttccagt catggcaaac ccgacaccca
     8821 ccatggctcc atagattatg ccatctaaca cattgtcaaa ctctcggcga tagcgaaagg
     8881 caacatagac tacagctact ccctttagaa tttcttcagt taatggagag agcaggataa
     8941 cccataacgc tcttgtagcg tttggggtaa ataaccccgg cggaagacgg taaaaaatcc
     9001 gcataatgat agccaatacc aaagctggta aagcccccca tagaaaggta gaaaccagaa
     9061 gacgacttgg ctctttctcg taatagtcgg accaatacag caagctcaca tagaccagtg
     9121 cgggcaagac agctgccaag agtccaatac cgataccttt gaccaggtca ttggttgata
     9181 gcgaaccagc catccagacc cagtctgggt cataacccga aagcgcctcg gtaatattct
     9241 cgtcaggctg tagctcatag gtcttgccat caacgcgcaa caatggcaaa tgatcattgg
     9301 ttagggtgat tgaaatatgc ccggaaaggc tcaatggcag atcggcaaaa gagaaatgat
     9361 tgacgcgaga agccactatg tcaggccaag taacatctag gttgtagggt gtattgcgag
     9421 gcaattcaat gtcagcgatg atctcgtcca gaggattaag attggtgtga gacagggcaa
     9481 aataccctg
//
LOCUS       DBNGSX010000392         9273 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1420150, whole genome shotgun sequence.
ACCESSION   DBNGSX010000392 DBNGSX010000000
VERSION     DBNGSX010000392.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9273)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9273
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1420150"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2220
                     /locus_tag="AC16CB_11680"
     CDS             <1..2220
                     /locus_tag="AC16CB_11680"
                     /inference="COORDINATES: protein motif:HMM:NF013109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902358 - sulfate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR3343175.1"
                     /translation="TLQARLTTWMADELRPGQLVRSLTTSLLVYILEIIVAISFAALI
                     FSGELSSQLPYGIGLILIGDMLLVLCVSLFSSYSGSIAVEQDAPGVILALIAATVVAA
                     LPAGANQTEKFSTVVMMIVITSVGTGLFFMVLGLFKLGGLVRYLPYSVMGGFLAGTGW
                     LLAIGGVSLMADASSLSLTLFQPDLLIRWLPGVLLALVMLLAVNRSGNPLVLPVIFAL
                     GIGFFYAIAWMTRTSFAELNTQGWLLGSFPSGGLWQFPLQSELLSNVNWPILSNQIVN
                     LSPVLIVSVIALLLNANGLELIIKKDVNLNRELITAGIGNLAAGLGGGIVGYHAISLS
                     SLNHTASGGKRLVGLFTALLVGITVFLGAGYLSYAPKMVLGALLVFLGLSFLYDWVYQ
                     AWFKFPKSEFVIILTILLVIAFRGFLEGIALGLVMAIILFVVNYSQVKVVKQTLYGTT
                     YRSRVTRSRSQWEILTARGDELCIFKLQGFIFFGTAISLFEQVRDLTRRTDSRPMRFA
                     VLDFAQVTGLDSTALLSFNKLLDLAQEKSFTLVLTGLTGRASEQFEKGGFQAQMEALQ
                     LFADLDHGIEWCENQILADAPVDIEAESSLQAQLAAILPGEQTRIERLIGYLERKEIA
                     PGEYLIRQGDAPDMMYFIESGQVTARLEAPGQKPVRLETMGGGRTIGELGFFLGVKRT
                     AAVVADKPSVVYGISKDMLRQMEEIHPEEANTFHRIIIQLLGERTVHLIRSVDALQR"
     gene            2222..3628
                     /locus_tag="AC16CB_11685"
     CDS             2222..3628
                     /locus_tag="AC16CB_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3343176.1"
                     /translation="MKRLLPLLVLILFLLPSRVLADIAPPDQPAGANPIPGSEATQVR
                     MLSERVLIDVQAKAPQGSLGQAKVTASFSMRNLGSAVEMLPVRFPLSYPGGVAGGIQG
                     NLEIRDFAARVGGKAVTTRRIIGADSQPWAEFDVTFPVESDIQVEVAYLVTGVGEYPF
                     IAFYYILETGAGWQGTIGSADLVLRLPYEANSLNVIFDEELGWSKTTPGGLLSGQEVR
                     WHLEDFEPSKGDNLQVSLVMPAAWNAVLTERANVARNPDDGEAWGRLGKAYKEILFLR
                     KGVRQDAGGQELYRLSQEAYENALERLPKDALWHAGYADLLYQAYYWKEFFTAQPDHS
                     AMLRALEQLRVSIELDPQNTKAIELLEDMKYALPEAVRQEGDTDLLLWLTATPTARPT
                     FTNTPSQTPLPPTATGTPMPTPALERSPTETPTHTPQLPTTESAATPEPPSGSPVCGT
                     AALLPLAVIAWVWKRRER"
     gene            complement(3633..4670)
                     /locus_tag="AC16CB_11690"
     CDS             complement(3633..4670)
                     /locus_tag="AC16CB_11690"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR3343177.1"
                     /translation="MRPPRFPIILITAFTLLVTLSCALGNRLQITYQTPATASVPPEF
                     FTPTPGEPLSTEEAEAPSSEAPSESEPPSVSEEFAPCEKKVCIESGSFLLKRPIGPDG
                     RFTIDTSSRFGTLRRRLRDAYLGVQFLNSTGTPVLAAWDGDVVVAGEDSRISYGVRPN
                     MYGNLVILRHFLPGIPEPVYTLYAHLSEVSVKAEGDVEAGEQIGLVGMTGSIFGSTLY
                     FEVRYGENSPEAVRNPELWLELLPDETGDSMGALAGQVVDRQGNYVSLSNILVENLRS
                     ASQGLDRQIYLKTYIEKDLMGQSPWGETFAVSNLPEGTYQISFWYGAELYQRQVEVQP
                     GMLTFVPFEVK"
     gene            complement(4975..7362)
                     /locus_tag="AC16CB_11695"
     CDS             complement(4975..7362)
                     /locus_tag="AC16CB_11695"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR3343178.1"
                     /translation="MIRPRWRKVIRDIWENKVRTLLVVASIFIGIFSVGLTAAMYIMT
                     GEDLNTSWIATNPAHAYIYTSTYQPEFLERVRRLPGVSGAEGRRGVTTRLKIGPDEWI
                     PLFLTTKPTYGHSQIDVLKPEAGAWPPGDKELVIERSSLRWANAEIGDTLEIKLIDDT
                     TRKIPLVGVVHDAQAGPGSALGISGYVTFDTLEWLHTERNLSSIAITVSENALDQNHI
                     QAVADGVAERVRESGGEVYYVYVPPPGKHPGYDSVLGVIAIIGALGFMSLFLAGFLIF
                     NTFSALMVQHVRYIGMMKAIGARRSQIVGMYLVLIMAFSLFALLPAIPLSAYAASWVA
                     VMMSQQFNYESLGFRMVPAVVLLQVVVGLVIPLLAGILPVYTGTRITIHKALSSYGLG
                     NSEIRRSLVDRLVESIRGLSRPLLISLRNTVRRKGRLVLTMATLALGGAIFISVFNLR
                     ESLVVFVEQIGKYYLADANVSLQQPYRLQELEQYIMQIPGVKRVEGWAGANAELLRAD
                     GVGTDNVSLIAPPVDSDLIEPVMLRGRWLTPGDQNAIVVSNAFWNTLPDLKVGDEIKL
                     DINDKETTWQVVGFFKFPGDSGLLAYTSYEYLTDLLHSPNRAYSVRIVGEDHSPAAQK
                     KLAKQVEQVLKSRGIQLVGQIQTSSMMTEANAQVINIIVGFFLFFAILVAVVGAIGLM
                     GTMSMNVLERTREIGVMRAIGATNGAVFRIVLVEGMLIGFISWVLGVLAATPITNLLY
                     DILSRAIFQTEGVAVMTWDGFIIWLVLAVVLSAIACLIPARNAARMTVRDVLAYE"
     gene            complement(7359..8525)
                     /locus_tag="AC16CB_11700"
     CDS             complement(7359..8525)
                     /locus_tag="AC16CB_11700"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3343179.1"
                     /translation="MFSRVFQRKKVDRINLNHIGGNGKKPIIKLDRLVKTYSSTAGEF
                     MVLKEIDASIYEGEFVSVIGRSGSGKSTMLNMIAGIDRPTSGDVQVGDVSVHELSESD
                     MAAWRGRNLGIVFQFFQLLPMLSLIENVMLPMDFCNVYTPRQRKERAMELLRKVELDR
                     HARKLPSAMSGGEQQRVAIARALANDPPIILADEPTGNLDSKTADVVFRLFKDLVDQG
                     KTVVMVTHDSSQAQRATRTMLLVDGEIMNEYVARALPTLTPEQLVKATRSLQPMHFEA
                     GATILQEGMPGENFYIITQGRAEVTLRRPEGTDVVVARLGEGQYFGEIELMQGRKNAA
                     SVRASEHSPVELVALNQAAIDSILDAADPTRAVLEKAAADHIAENIEARKEEQA"
     gene            8909..>9273
                     /locus_tag="AC16CB_11705"
     CDS             8909..>9273
                     /locus_tag="AC16CB_11705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098015 - virus tail [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HIR3343180.1"
                     /translation="MTPTAGRHDPYRNFNFIVEIDGIASSGFLMVDGLESITEVVDYR
                     EGNEKATPRKLTGISKASNITLKRGVTDNRDLWEWRKTVLDGTPQRRNGSVVILNESR
                     EQVLRVNFQNAWPCRWKIGG"
BASE COUNT         2109 a         2604 c         2471 g         2089 t
ORIGIN      
        1 accctgcaag cgcgtctaac tacctggatg gcggatgaac ttcgcccagg gcaattggtt
       61 agaagcctga ccaccagcct cctggtttat attctagaaa ttatcgtggc tatctctttc
      121 gctgcactga ttttctcggg agagctgtcc agccaactcc cttatggtat tggcttgatt
      181 ctgatcggag atatgctttt ggtcctttgt gtctccttat tcagctcgta ttcaggctcg
      241 attgccgttg aacaggacgc acccggagtg atattggctt tgattgccgc tactgtggta
      301 gctgcgctgc ccgcaggagc caaccaaact gagaaattct cgaccgttgt gatgatgatc
      361 gtaataacca gcgtggggac cggcttgttt tttatggtgt tggggttgtt taagctcggc
      421 gggctagtgc gctacctgcc ttactcggtg atgggcggct tcctggctgg cacaggctgg
      481 ttgctagcca taggtggtgt gagcttaatg gcagatgcct catcgttgag ccttacctta
      541 tttcagcccg atctgctcat ccgctggctc cctggggtgt tgcttgctct ggtgatgctc
      601 ttggccgtca atcgttctgg caaccccctg gtattgcctg tgatattcgc acttggaatt
      661 ggcttttttt atgccatagc ctggatgacg aggacatcat ttgcagagct taacacacaa
      721 ggctggctgt tggggtcatt tccatctggg ggcttgtggc aattcccttt gcaatcggaa
      781 ctattatcca atgtcaactg gccaattctc tccaaccaaa ttgtcaacct atcaccggtg
      841 ttgatcgtca gcgtcatcgc cctgctacta aatgccaacg gtctcgaatt gatcatcaaa
      901 aaggatgtta atttgaatcg cgagcttatt actgcgggga tagggaatct ggctgctggt
      961 ctgggtggcg ggatcgtcgg ctaccatgcg atcagcttat ccagcctgaa ccacactgcg
     1021 agtggtggca aacgtctggt aggcctgttt actgcgctgc tagtaggtat aacggtattc
     1081 ctgggcgcgg gctatcttag ctatgcccct aaaatggtcc tgggcgcact ccttgtcttc
     1141 ttagggctct cattcttgta cgattgggta taccaagcct ggttcaagtt ccctaaaagc
     1201 gaattcgtga ttatcctgac catcttgctg gttatcgcat tcaggggatt tctggagggg
     1261 atcgcactcg ggctggtgat ggccatcatc ctgtttgtcg tcaactacag ccaggtgaaa
     1321 gtggtcaaac aaacattata tggcacaacc taccgcagcc gggtgacccg cagtcgcagc
     1381 cagtgggaaa tcctaacagc ccgaggggat gaactatgca tctttaaact ccagggattc
     1441 atcttcttcg gaaccgctat aagcctgttc gaacaggtcc gcgatctgac gaggcgcaca
     1501 gattcaaggc caatgcgctt tgccgtgctg gatttcgcac aggtcaccgg tttggactca
     1561 actgccctgc tcagctttaa caagctgctt gacttggctc aagaaaaaag ctttaccctg
     1621 gtgctaaccg ggctgaccgg cagggccagc gagcagttcg agaaaggcgg cttccaggcc
     1681 caaatggaag ccttgcagct cttcgcggat ctcgatcatg ggatcgaatg gtgtgaaaac
     1741 cagatacttg ctgacgcacc tgttgatatt gaagcagaaa gcagcctaca ggcacagctg
     1801 gcagccatct tgcccggtga gcagaccaga atcgaaagat tgatagggta cctggagcgc
     1861 aaggaaattg ccccaggaga atatctgatc cgccagggag acgctccaga tatgatgtat
     1921 tttatcgaat ctggtcaagt caccgcgcgg ctggaggccc ccgggcaaaa accggtgcga
     1981 ttagagacga tggggggcgg ccgcaccatc ggagaactgg gattcttttt gggggtcaaa
     2041 cgcacagctg cggttgtcgc cgataaaccc agtgtggtgt acggcatctc gaaagacatg
     2101 ttgaggcaaa tggaggagat ccaccctgaa gaggccaaca cctttcaccg catcatcatc
     2161 cagcttttgg gcgaaagaac tgtccacctg atcaggtcgg ttgacgcctt gcagcgctga
     2221 catgaaaaga ctgctgcctc tacttgtact gatcctattt cttcttccca gtcgggttct
     2281 ggctgatatc gccccaccgg accagccagc cggcgccaat ccaatccccg ggagcgaggc
     2341 cacacaggtg cgcatgttgt ctgaacgtgt gctgatcgat gtgcaagcca aagctccgca
     2401 gggctccctg ggccaggcca aggttaccgc cagcttcagc atgcgaaacc tggggagcgc
     2461 agtagagatg ctacccgtgc ggttcccgct ctcttacccg ggaggtgttg cgggcggcat
     2521 ccagggcaac ctggagattc gggattttgc agcgcgtgtg ggcggcaaag cagtcacgac
     2581 ccgccgcatc attggagcag actcccagcc ctgggccgag ttcgatgtga ccttccccgt
     2641 ggaaagcgat atccaggtgg aggtcgccta tctggtcact ggtgtgggcg aatatccctt
     2701 catcgccttt tattacatct tggaaaccgg agctggatgg cagggaacca tcggcagcgc
     2761 cgacctggtc ttgcgcctgc catacgaagc caacagcttg aacgtgattt ttgacgaaga
     2821 gctgggctgg tcgaagacca cacccggcgg gctgctaagc ggacaggaag tgcgctggca
     2881 tttggaggac tttgagccct ccaagggcga caacctgcag gtctccctgg tcatgcctgc
     2941 agcgtggaac gcagttttaa ccgagagagc taacgtcgcc cgcaatccgg atgatggcga
     3001 ggcctggggg agactgggca aggcctataa agagatcctt ttcctgcgca agggtgtgcg
     3061 ccaggatgcc ggtgggcaag agctttatcg gctgagccag gaagcctatg aaaatgcgct
     3121 ggaacgcttg ccgaaagacg ctttatggca tgcaggatat gccgatctgc tgtaccaggc
     3181 ctactactgg aaggagttct tcaccgcaca accggatcac tcggctatgc tgcgcgcatt
     3241 ggagcagctc cgcgtgtcca tcgagctgga cccgcagaac acaaaagcca tagagcttct
     3301 ggaggatatg aagtacgccc tgccagaggc cgtcaggcag gagggagata ctgacctgct
     3361 gctctggctg accgccacac ccaccgcgcg gcccaccttt acgaatacgc ccagccaaac
     3421 acccctgccg cccaccgcga cgggcacgcc catgcccacc cccgccttgg agcgatcgcc
     3481 gaccgaaacc cccacccaca cgccgcaact accgactacg gaaagcgctg ctacacctga
     3541 gccgccctca gggtcaccag tttgcgggac agccgcgctg ctaccgctgg ctgtgatcgc
     3601 ttgggtatgg aagcgacggg agcgctaatg ctttacttca cttcaaacgg cacaaacgtc
     3661 agcatgccgg gttgcacttc cacctggcgc tggtagagtt ccgcgccata ccagaatgaa
     3721 atttgatagg tgccctcggg tagattgctg acggcgaaag tctcgcccca ggggctttgt
     3781 cccatcaggt ctttttcgat gtaggtcttc aaatagatct gacggtccag accctggctg
     3841 gcggatcgca aattttcgac caggatattg cttagcgaca catagttccc ttgacgatcc
     3901 accacctgcc cggcgagcgc gcccatcgag tcgcctgttt catcggggag cagctccagc
     3961 caaagctccg ggttgcgtac cgcctcagga gagttctctc catagcgcac ctcaaaatac
     4021 agggtgctgc cgaatataga gccagtcatc cctaccagcc cgatctgctc tccggcttct
     4081 acatcaccct cggccttgac cgagacctcg gacaggtgag cgtacaaggt gtaaacaggc
     4141 tcgggaattc cgggcaagaa atgccgcaag atgaccaggt tgccatacat attggggcgc
     4201 acgccatagc tgatccggct gtcctcccct gccacgacca catccccatc ccaggctgcc
     4261 aggaccgggg tgccggttga attgaggaat tgcaccccga ggtaggcgtc gcgcagacgc
     4321 ctgcgcaagg tgccgaatcg agatgaggtg tcgatggtaa atcgcccatc tggaccaata
     4381 ggccgcttca gaaggaatga gccgctctcg atgcacacct tcttctcgca gggagcgaat
     4441 tcttctgaaa cggacggagg ttcgctttcc gatggcgcct ctgatgaagg agcttcagcc
     4501 tcttccgtgg aaaggggttc gcctggcgtt ggtgtaaaaa actccggagg gacgctggct
     4561 gtcgctggcg tttgataggt tatttgcaga cggttaccca gggcacaact gagtgtgacc
     4621 agcaaggtaa acgccgttat gagaataatt gggaatctgg ggggtctcat cgcctggata
     4681 cctccgatcc tataagggcc tggtttctgg tgctaaatgt taggattgtt gcccaaccac
     4741 agtgaaactg cgattgaaac ggccttgaac tgccgttcca caaaaaacta gcctagcgac
     4801 ccgatggcac attcgcctga tgagcaaaaa acggatgggt actcctgctc acatattata
     4861 tattcaacat aaaatataac acttaagtat gcgttatgta tatattaaat gacttgcgca
     4921 atttatgtgt ttgaacgaga tttcggggaa tcagcaatat tagcggcagg gcggttattc
     4981 gtacgccaag acgtctcgca ccgtcatgcg cgccgcattg cgggccggta tcaggcaggc
     5041 gatggcggac aggaccacag ccagcaccag ccagatgatg aaaccatccc aggtcatcac
     5101 cgccacgcct tccgtctgga atatcgcccg gctcaggatg tcgtacagca aattcgtgat
     5161 gggggtagcc gccaatacgc ccaaaaccca gctgataaac ccgatcaaca tcccctcaac
     5221 aagcacaatg cgaaaaactg cgccattggt ggcgccaatc gctcgcatca cgccgatctc
     5281 gcgggtgcgc tccagcacat tcatgctcat ggtgcccatc agaccaatcg cccccaccac
     5341 ggccaccagg atggcgaaga acaagaaaaa acccacaata atattgatca cctgggcgtt
     5401 ggcctccgtc atcatgctac tggtctggat ctggccaacc aactggatgc cgcggctttt
     5461 caggacctgt tcaacttgct tagccagttt tttctgggct gcgggggagt ggtcttcgcc
     5521 aacgatacgg acggagtatg cccggtttgg agagtgcaat aagtctgtca ggtattcata
     5581 gctggtatag gcgagcagcc ccgagtcacc cggaaactta aagaagccca ccacctgcca
     5641 ggttgtctct ttgtcattga tgtcgagctt gatctcatct cccaccttga ggtctggcaa
     5701 cgtattccag aaagcgttgc tcaccacgat ggcgttttga tcgccggggg tcagccagcg
     5761 gccgcgcagc atcaccggct cgatcagatc gctatccacg ggcggagcga tgagggacac
     5821 gttatcggtg ccgacgccat ccgcgcgcag caattcagcg tttgcgcccg cccagccttc
     5881 cacgcgcttc acgccgggaa tttgcattat gtactgctca agttcctgta gtcggtaagg
     5941 ttgttgcaag ctgacgttgg catctgccaa gtagtacttg ccgatctgct cgacaaaaac
     6001 caccaaagac tctcgcagat tgaaaaccga tataaaaata gcacctccga gcgccagtgt
     6061 agccatggtg agcaccaggc gacccttgcg ccgcaccgtg ttgcgcagag agatcaggag
     6121 cgggcgcgat agcccacgga tactttctac cagccggtcc acaaggctgc ggcgaatctc
     6181 gctattcccc agcccatagc tgctgagcgc cttgtggatg gtgatgcgcg tgccggtata
     6241 cacaggcaga atgcctgcca gcaacggtat taccaatcca acaacaactt gcaagagaac
     6301 taccgctgga accatgcgaa aaccaagcga ttcatagttg aactgctggc tcatcataac
     6361 cgccacccat gaagctgcgt aggcagagag cggtatggcg gggagcaagg caaataagct
     6421 gaaggccatg atgagcacca gatacatgcc cacaatttgt gagcggcgcg ccccaatggc
     6481 tttcatcatg ccaatatagc gcacgtgctg caccatcaat gccgaaaatg tgttaaagat
     6541 caagaaaccc gccaggaata gcgacataaa gcccagcgcg ccaatgatcg cgatcacccc
     6601 cagcactgag tcatacccgg gatgctttcc cggtggcggg acataaacat agtacacctc
     6661 tcccccgctc tcacgcacgc gctcggctac accatcggca actgcctgga tatgattctg
     6721 gtccagcgca ttttcggaga cggtgatggc gatgctagat aggtttcttt cggtatgcag
     6781 ccactccagc gtatcaaagg tgacatagcc gctgatcccg agcgcggaac ccggacccgc
     6841 ctgggcatcg tgcacgacac ccaccagcgg gattttgcga gtggtatcat cgatgagctt
     6901 gatctccagc gtatcaccaa tctctgcatt agcccaccgc aagctgcttc gctcaattac
     6961 cagttcctta tcgcctgggg gccaggcgcc ggcttctggt ttaagcacgt cgatctggga
     7021 atgaccatag gtgggttttg tcgtcaggaa gagagggatc cactcatccg gtccaatctt
     7081 caagcgagtg gtgactcccc gcctgccctc agcccccgac acaccaggca agcggcgcac
     7141 cctctccaaa aactcgggct gatatgtgct ggtatagata tatgcatgag ccgggttcgt
     7201 cgctatccag ctggtattca aatcttcacc ggtcataata tacatggcgg cggttagacc
     7261 cacgctgaaa ataccgataa agatcgatgc cacaaccagc agcgtgcgca ctttgttttc
     7321 ccatatgtca cggatgactt tgcgccagcg cgggcggatc atgcttgctc ctccttgcgc
     7381 gcttcgatat tttccgcaat gtggtccgcc gcggctttct ccagcacagc acgtgttggg
     7441 tctgccgcat ccagaatcga atcgatggca gcttgattca gggcaaccaa ttcaaccggg
     7501 gaatgttcgg aagcccttac gctggccgcg tttttcctgc cttgcatcaa ttcgatctca
     7561 ccgaagtatt gtccctcacc caggcgagcg acaaccacat cggtgccttc cggtcgtcgc
     7621 aatgtcacct cggccctccc ctgtgtgata atgtaaaaat tctctcccgg catcccctct
     7681 tgcaagatgg tcgcgccagc ctcaaagtgc atgggctgga ggctgcgggt ggctttgacc
     7741 aactgttcgg gggttagtgt aggcaatgcc ctggctacat attcattcat gatctcgccg
     7801 tccaccaata acatggtacg ggtagcccgc tgtgcctggc tgctatcatg cgtcaccatg
     7861 acgactgttt ttccctgatc gacgagatct ttaaagagac gaaacaccac atctgccgtt
     7921 ttggagtcca ggttgccggt tggctcgtct gccaggatga tcggggggtc gttggctagc
     7981 gcacgggcaa ttgccacgcg ttgctgctca cctccagaca tcgcagaagg gagttttctg
     8041 gcatgccggt ctaattccac cttgcgaagc aactccatag cccgctcttt gcgctgccgc
     8101 ggggtataca cattgcaaaa atccatgggc aacatcacat tctctatcaa ggagagcatc
     8161 ggcaataatt ggaaaaattg gaagacgatg cctaaattgc gacctcgcca ggcagccata
     8221 tcgctttcgc tcaattcgtg gacggacaca tcgcccactt gcacatctcc agaggtaggg
     8281 cggtcgattc cggcgatcat gttcagcatg gtcgattttc cgctgccgga cctgccaatt
     8341 acagatacga actcaccctc atagatgctg gcatcgatct ctttcaatac catgaattcg
     8401 cccgccgtgc tggaatatgt tttaacgagt ctgtccagct tgatgattgg ctttttacca
     8461 ttgcccccaa tatgattaag gttaatgcga tcaacctttt ttcgttgaaa aaccctggaa
     8521 aacattactt cctccccgga atacagctac tcctttagaa tttaaaatca aataggtcta
     8581 cagctcaacg tcctatctat atgtatatac taaaatttcc aagaaaagtt tcagaaccct
     8641 ggccatcatg ggctattcaa acatcgctca tgtggggaga ttaccctccg tgacataaat
     8701 ttaaggcacg aattttaggg cgcaatcaat aggcactcgg ctgatataat acccgcattg
     8761 aataagccgc gagctcaagt gtttcttcgc cgacaccatc gtatgggtat cccaacggga
     8821 cattactctc tgcacgtatt cggaggattg gatgccacca tctcgccagc ccaagcgcaa
     8881 accccgcaag ttgaaaaagg aggaaaaaat gaccccgact gcaggtcgtc atgacccata
     8941 tcgaaatttc aattttattg tcgagattga tgggatcgca tcctcaggtt tcctgatggt
     9001 ggacggtctc gaatcgatta ccgaggtggt ggattaccgc gaaggcaacg agaaggctac
     9061 gccgcgcaag ctcaccggaa tctctaaggc ttccaatatc accttgaagc gcggtgtaac
     9121 cgacaaccgg gacttgtggg aatggcgcaa aaccgtgctg gatgggaccc cacagcgccg
     9181 taatggatct gttgtcatcc tgaatgaatc gcgcgagcag gtcctgcgtg tcaacttcca
     9241 aaacgcctgg ccttgccgct ggaaaatcgg cgg
//
LOCUS       DBNGSX010000393         1671 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1074283, whole genome shotgun sequence.
ACCESSION   DBNGSX010000393 DBNGSX010000000
VERSION     DBNGSX010000393.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1074283"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="AC16CB_11710"
     CDS             complement(<1..353)
                     /locus_tag="AC16CB_11710"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3343181.1"
                     /translation="MKQRLPYPELAVVIASAAWGLFWIPLRAFERRGLEPAWATLTQF
                     LAPLAILLPVAVIRWQRRQPTGIHQYRTGLLIGAAVALYLESLLLTDVARALILFYAM
                     PAWGTVLEVGLMGRPL"
     gene            737..1165
                     /locus_tag="AC16CB_11715"
     CDS             737..1165
                     /locus_tag="AC16CB_11715"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR3343182.1"
                     /translation="MSEENKAIIRRFYEELDKGNFDIYDELCTPDFISHFPGNPQPID
                     RETRKQTSRMFYAAIPDCKHTLEDTIAEGNKVSFRGSARGTHTGAFRGQPPTGNSVMF
                     TGMRFYRIVDGKIAEEWASFDSLALMQQLGVIPALEESKE"
     gene            1305..>1671
                     /locus_tag="AC16CB_11720"
     CDS             1305..>1671
                     /locus_tag="AC16CB_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3343183.1"
                     /translation="MTVKLQVSIVIDRPVANVFHFYAHEHVRNHPRWDPDMQLEQISD
                     GPIGIGTLIRRRNTHSGTPVDGTMEVVEFEPNRAFGVLIQDGPSETHGRVTFKAESPK
                     QTTLTIHANFPDMDESLEAW"
BASE COUNT          470 a          425 c          421 g          355 t
ORIGIN      
        1 agcggacgcc ccatcaggcc cacttctaaa actgttcccc aggctggcat ggcgtagaag
       61 agaatcaatg cacgtgctac gtcagtgagg agcaggctct caagatacaa cgcaaccgca
      121 gcaccgatca gcagtccagt acgatactgg tggatacctg tgggctgtct acgctgccac
      181 cttatgaccg cgaccggcag aaggatggca aggggcgcga gaaactgggt cagggtagcc
      241 caggcaggct cgaggccgcg gcgttcaaac gctctcagag gaatccagaa caatccccat
      301 gcagctgatg caattacgac ggcaagttca gggtagggaa gtctttgttt catttcgact
      361 ggaggagatt tctttacatt ggccaggaat agagagatag taaataaaag tatacactaa
      421 caaattcaga caaaagacaa aagaagtgat agccgatttt gtgggctttc gatacttgct
      481 gcacaaaata atgattatgt ttaccttaat aatcgcattc gattataatc aatatagata
      541 acaccacaag ctacacgaac cacgttttaa tctgtggtcg tataccccct gggtttatct
      601 tggtgatgag gcagcataaa atatatatcc cacatcccgc acctggttgt gcaacattaa
      661 tctaccctgt caaatctcag cacatataat caccaacact acccaaattt agcttaagcc
      721 agacaggagg cttttgatgt cagaggaaaa taaggccatc atcaggcgct tctatgaaga
      781 gctcgataaa ggcaattttg atatttacga cgagttgtgt acccctgact tcatctcgca
      841 ctttcccggc aacccccagc ccatagaccg ggagacccgc aagcaaacct cccgcatgtt
      901 ctatgcggct atacccgact gtaagcatac cctggaggac acgatagccg agggcaacaa
      961 ggtctctttt cggggctctg cgcgcggcac tcacacggga gcatttcggg gccagccgcc
     1021 gactgggaac tcagtcatgt tcaccggcat gcgcttttac cgcatcgttg atggcaagat
     1081 cgcggaagaa tgggccagct tcgacagcct ggcgctgatg cagcagctgg gcgtgattcc
     1141 agcccttgaa gaaagtaaag agtaaagcta ccggctggat tttatgtgac aaaatgccca
     1201 ggagcgtatg aagacataca ctcgggcttt tgcatcaagc tgaagcatta gaatgaactt
     1261 agattatgta ggcggtgtca acgccaaacc taaaggaaag aacaatgaca gtgaagcttc
     1321 aggtttcgat agtgatcgac cgaccagtgg ctaacgtttt ccacttctac gcccatgagc
     1381 acgtccgcaa ccatccgcgc tgggacccag acatgcaact cgagcagata tcagatggcc
     1441 cgatcggcat agggaccttg atccgcaggc gcaacaccca cagcggcact ccggttgatg
     1501 ggacaatgga agtggtggag tttgagccaa atcgagcgtt tggagtgtta atccaagacg
     1561 gaccaagcga aacgcacggt cgggtgacat tcaaggctga aagtccaaag caaaccaccc
     1621 tcacgatcca cgcaaatttc cccgacatgg atgagtcgtt ggaggcttgg a
//
LOCUS       DBNGSX010000394         6072 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_659135, whole genome shotgun sequence.
ACCESSION   DBNGSX010000394 DBNGSX010000000
VERSION     DBNGSX010000394.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6072)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6072
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_659135"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="AC16CB_11725"
     CDS             complement(<1..440)
                     /locus_tag="AC16CB_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HIR3343184.1"
                     /translation="MIGTIINIFTVLLGGSLGVLVGARLPEKIRSTVVAGLGLFTAAI
                     GVQMFMQSGNVIIVLGSVLVGGILGEWLRIEDGLQGLGSYLEQRFAGGKSADQGSRFI
                     RGFLTSSLVFCVGPMTILGAIQDGMQGDYSLLAIKSVLDGFASLA"
     gene            complement(437..988)
                     /locus_tag="AC16CB_11730"
     CDS             complement(437..988)
                     /locus_tag="AC16CB_11730"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR3343185.1"
                     /translation="MSKFYTRTGDDGTTGLLGEGRVPKHHVRIEAIGSIDEASAAIGA
                     ARAICKGEGVNALLQSVQRDLYDLMAEIAATKENLPRFQSINMERVAWLEGEIDRISA
                     EVKLPKGFILPGDSPASGLLALARTIVRRAERRVSRLLHEGQVENVHLQRYLNRLSSL
                     CFALELVENQAAGLDSINLANPE"
     gene            1240..1467
                     /locus_tag="AC16CB_11735"
     CDS             1240..1467
                     /locus_tag="AC16CB_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343186.1"
                     /translation="MQIRCYHCHKPFALGKEAVYQALQEMKTSEMSHYNAYCPHCRRA
                     NRVSHQELLRAAPDWTPEEETTDQGESVSSS"
     gene            1493..2464
                     /locus_tag="AC16CB_11740"
     CDS             1493..2464
                     /locus_tag="AC16CB_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3343187.1"
                     /translation="MKNVRSFSSAEELKKALGGQQYIASDEIATIMYLSQELGKPLLT
                     EGPAGVGKTELAKAIAGATGRELIRLQCYEGLDETKALYEWEYAKQLLYTQLLRDKLQ
                     DTLAGASNLTEAADRLAGEEDVFFSMRFLLQRPLLKAILSEKPTVLLIDEIDRADAEF
                     EAFLLEVLSDFQVSVPELGTLVAVHRPLVVLTSNNTRELSEALKRRCLYLFINYPTIE
                     QELAVVRLKVSELSNKLARQVVEMVQNLRSMDLRKSPSISETIDWAKALVSLNAKSLD
                     QKTLETTLSVLLKHESDLQRVRRWMETPPSTGRPGDPDDPRMARWRN"
     gene            2543..3925
                     /locus_tag="AC16CB_11745"
     CDS             2543..3925
                     /locus_tag="AC16CB_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3343188.1"
                     /translation="MEERIVKFITALRSAGVRVSLAESADAFTAVDLLGVRNRDTFRL
                     TLRATLVKDAKNIPIYEELFPLFFGSADAPPLMNLSDDLTPEEAEKIAQALRQFSEKI
                     RQMLERLMRGEQLSQEELERLGKFVGLNHIDDLRYREWMTQRLQKALRFAEVREALQE
                     LGELLAQMGMDKQRVEQMMHLMQANQKALEDQLRQYAGQRIAENLSEQRPDDSLDSLL
                     NRPFGTLSDKDMDRLRKEVQRLATVLRTRVALRQKRAKSGQLDAKKTIRANLKHGNVP
                     IEIKHREHSLKPKLVVICDVSTSMRSCSELMLSLLYALQDQISKTHAFAFIDHLEYIS
                     PDFVSDEAREAVQHVLARMPSGYYNTDLGNSLENFSQDFLDTVDGRTTFLVVGDGRNN
                     YNDPRVDIFSTLARRSRRTIWITPEAPGLWGSGDSDMLKYAPACDVILKAGNLAELTT
                     AVDKLLAPHT"
     gene            4020..4409
                     /locus_tag="AC16CB_11750"
     CDS             4020..4409
                     /locus_tag="AC16CB_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343189.1"
                     /translation="MIIKIFQGLVLLGFGVFQASQFGWGWGWEGSELVFTPLVPYSVI
                     DLAYSGLLYLILAIITLFIALELWLVRRWAWLAAMTLQGLSLLAALFGYLQGQPNYIS
                     MALGIVLVLYLNQAEVQAVFRRSPKSA"
     gene            4409..>6072
                     /locus_tag="AC16CB_11755"
     CDS             4409..>6072
                     /locus_tag="AC16CB_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343190.1"
                     /translation="MDKSGDLALLRRFEPVLRFTRGERFFPMDVERYIGQCSLWMQQE
                     GQEPELLVPQGELTLDKLVEPRQIGFGAVHFLKFIEPLDILELARYSFDRAVSSIRQA
                     LKLSLQDPLHKFRAGRGRLARVGYGSRFVDALFSLTLMLRGRVPGDTAAAAALAYKRM
                     QDETERYCYYGRVARQNGWVILQYWFFYPFNNWRSGFFGVNDHEGDWEMISIYCSETH
                     SDGAELPSVDRLVPEWVAYASHDFSGDDLRRRWDDPEILKVPDAQGNLHPVVFAGAGS
                     HASYFSQGEYLTELELPFLSALVQLVDKLQSLWVKVLHQSSGSASSPGFNVFRIPFVD
                     YARGDGLALGPGQDREWQPEVISESTAWAISYRGLWGLYARDPIACENAPAGPVYNRD
                     GSVRQSWCNPLGWSGLDKLPPNHDAPEVLKRKLDEIQAYQVSLKTKIDQQSSKLVGMG
                     VEAAAMQGYPHLEQAHRAQEEQIATLSAEVHHLRQELAQVDARLDALRLYGEQLSQAD
                     PGPMRAHIHRAHHPSPESELRLASVAEFVAAVSIGALIVGIVLLIV"
BASE COUNT         1471 a         1451 c         1649 g         1501 t
ORIGIN      
        1 gccagcgaag caaagccgtc tagcacagat ttgatcgcca acaagctgta atcaccttgc
       61 attccatctt gaatggcgcc caagatggtc ataggaccca cacaaaagac cagagaagaa
      121 gtcaagaatc cacgtataaa acggctgcct tggtcagcgc ttttgccgcc ggcgaaacgc
      181 tgttctaaat aactgcccag accttgcaag ccatcctcga tgcgcaacca ttctcccagg
      241 ataccgccga ccaaaacgct tcctaggacg ataatgacat tcccagactg cataaacatc
      301 tgcaccccga ttgctgcggt aaaaagtccc aagccagcca ccacggtgga tcgtattttc
      361 tctggcaatc tggcgcccac caggacacca aggctgcctc cgaggaggac ggtgaagata
      421 tttattattg tcccaatcat tctggatttg cgaggttaat ggagtctaga cctgctgcct
      481 ggttttccac cagttccagg gcgaagcaca aagacgataa gcggttcaag tatcgctgca
      541 aatgaacatt ttccacctgg ccctcgtgca gcaaacggga gacgcgccgc tcggcgcggc
      601 ggacaattgt gcgagccaac gccagcagcc cgctggctgg agaatcaccg ggcaggataa
      661 accctttggg taatttaact tcagcgctta tgcggtcgat ttctccctct aaccatgcca
      721 cgcgttccat gttgatgctt tgaaagcgag gtaagttttc tttggttgca gcaatctccg
      781 ccatcaaatc atatagatcg cgctgcacgc tctgcaatag agcgtttacc ccctcgccct
      841 tgcatatggc gcgcgcggcc ccaatggctg ctgaagcctc gtcaatgctg cctatcgcct
      901 caatgcgtac gtgatgtttg gggacacgtc cttcgccgag caagcccgtc gtaccatcat
      961 ctccagtccg cgtgtagaat tttgacataa gggcaattat acctggcttt tatagggcaa
     1021 tcgcatgagg gcgatttccc accactgaag agagaaaatg gggtgttttg gtgggggtat
     1081 atacccacca aaacacccca aacactatct ttgcgggatt gcgcatgcca ctcagggcat
     1141 atgcaattgc cctgctggct tttattttag tctatgtaga accgtataga tactgtatac
     1201 tatagattgt gactgagagt taggatagga gatcatgtca tgcagattcg atgttaccat
     1261 tgccacaaac catttgcctt gggcaaagaa gctgtctatc aggctctaca agagatgaag
     1321 acaagcgaaa tgagccacta taacgcctat tgcccgcatt gccgccgggc gaaccgtgtt
     1381 tctcatcaag aactattgcg cgcggctccc gactggactc ctgaggaaga aacaaccgat
     1441 cagggggaaa gcgtttctag ctcatagccc tttggtctta acaggtggat agatgaaaaa
     1501 tgttcgttct tttagctctg cggaagagct aaagaaagct ttgggtgggc agcaatatat
     1561 tgctagcgat gaaattgcta cgatcatgta cctcagccag gagttgggga agccacttct
     1621 gacagaagga cccgcgggtg ttggtaaaac tgaattggcg aaagcgatcg caggcgccac
     1681 ggggcgcgag ctgattcgtt tgcagtgcta tgaaggcctg gacgagacca aggcgctcta
     1741 tgaatgggag tatgccaagc agttgctcta tacccagctt ttacgtgata agctgcaaga
     1801 tacgcttgca ggcgccagca acctgaccga agccgccgac cgcctggcgg gagaagagga
     1861 tgttttcttt tcgatgcgct ttctattgca gcgccccttg ctgaaagcta tactcagcga
     1921 aaaaccaacc gtcttattaa ttgacgagat cgatcgtgct gacgcggagt tcgaggcctt
     1981 tttgcttgag gtgctgagcg attttcaagt cagcgttccg gagctgggta ccctggtagc
     2041 tgttcaccgc ccattggttg tgctgacgag caacaacact cgtgagctca gtgaagcgct
     2101 gaagcgtcgg tgcctgtacc tatttatcaa ttatcccacc atagagcagg agctggctgt
     2161 agtacggtta aaggtgtcgg agttatctaa taagctggct cgccaggtgg ttgagatggt
     2221 tcaaaacctg cgctcaatgg atttgcgcaa atcccctagt atcagcgaga ccattgattg
     2281 ggctaaggcc ctggtttcct tgaacgccaa gagcttggat cagaaaacat tggaaaccac
     2341 gttgagcgtt ctcttaaaac atgagagcga tttacagcgt gtccgccgct ggatggagac
     2401 acctccctcc actggaagac cgggggatcc tgacgatcca cgtatggcgc gatggcgtaa
     2461 ttgagggtga agggtctcac gggatctaca agaactcctc ttgctcgacc gggagccccc
     2521 caaaaatttt ataggtgctg gtatggaaga gcgtattgtc aaatttatta cagccttgcg
     2581 gtcagcaggt gtacgcgtaa gcctggctga gagtgcagat gcattcaccg cggttgattt
     2641 acttggtgtg cgcaatcgcg atacgtttcg attgaccctg cgggctaccc tggtaaaaga
     2701 cgccaagaac atcccaatct acgaggaact atttccgctt ttcttcggta gtgctgatgc
     2761 gcctccgttg atgaacctct cggatgattt aacgcccgaa gaggcggaga agattgctca
     2821 agctctgcgc cagttcagtg agaaaattcg ccagatgctc gagcgcttga tgcgtggtga
     2881 acaacttagc caggaagaat tggaacgcct gggaaaattt gttgggctaa accatattga
     2941 tgacctgcgc taccgggaat ggatgacgca aagattgcag aaggccctgc gatttgccga
     3001 agtgcgtgag gcattgcagg aactggggga gttactggct cagatgggca tggataagca
     3061 acgtgtcgag cagatgatgc acctgatgca agcaaatcag aaagcccttg aggatcagtt
     3121 gcgccagtat gctggtcaac gtattgctga aaatttgagt gagcagcgcc cggatgacag
     3181 cctggattct ctcctgaacc gtccttttgg caccctctcg gacaaagata tggataggct
     3241 tcgcaaggaa gtacaacgtc tggctacagt tctacgcaca agggtggcat tgcgtcaaaa
     3301 gcgggcaaag agcggtcagt tggatgccaa aaagaccatc cgcgccaatt tgaagcacgg
     3361 caacgtccca attgaaatta agcatcgcga gcatagcctc aagccaaaac tggttgttat
     3421 ctgtgatgtg agcacgtcca tgcgctcttg ttctgagctc atgcttagcc tcttatatgc
     3481 tttgcaagat cagatcagca agacacatgc ctttgctttc atcgaccacc ttgaatatat
     3541 ctcaccagat tttgtaagtg acgaggctcg tgaagctgta cagcatgtat tagcgcgcat
     3601 gccatcaggt tattacaata cagatcttgg caacagtctg gaaaatttca gccaagactt
     3661 tcttgatacc gtagatgggc gcaccacatt tttagtggtg ggagatgggc gcaataacta
     3721 taatgatccc agggtggata tcttcagcac gctagcccga cgcagcaggc gaacaatttg
     3781 gattacgcca gaggcaccag ggctgtgggg aagcggggat agcgatatgc tgaaatatgc
     3841 tcccgcctgt gatgtaatcc tgaaggcagg caacttagcg gaactgacca cggcggtgga
     3901 taaactgctt gctccacata cataataggt caacttcgcc tgatacaatt tttccaatgg
     3961 gactcaagaa actcgagaaa agaaaaatcg ttgagcgacc tcgtagcctg ggaatacttg
     4021 tgatcataaa gatttttcaa ggcctggtgc tactcggttt tggtgtattc caagcaagtc
     4081 aatttggatg gggatgggga tgggaaggca gcgaacttgt atttacgcct cttgtgccat
     4141 attcggtaat tgaccttgct tattctggtt tgctctattt gattttagcg atcatcacgc
     4201 tatttatcgc tttagaattg tggttggtac gcagatgggc atggctggct gccatgacgc
     4261 tgcaaggtct cagcttgttg gctgcgctat ttgggtatct tcaagggcag ccgaactata
     4321 taagtatggc acttgggata gtgctcgtgc tgtatctaaa ccaagctgaa gtccaggcgg
     4381 tatttcgccg ttcaccgaaa agtgcctaat ggataaatct ggagacctag cattattgcg
     4441 tcgcttcgag ccggtgcttc gctttactcg gggtgagcgg tttttcccga tggatgtaga
     4501 gcgatatatc ggccaatgta gcttgtggat gcaacaagaa ggccaagaac ctgagctatt
     4561 agtccctcaa ggtgaattaa ctcttgataa attggtagag ccgcgccaaa ttggatttgg
     4621 agcggtccat ttcctaaagt ttattgaacc tcttgatatt ctggagctag cacgctattc
     4681 cttcgatcgg gcggtctctt caatcagaca ggcattaaaa ctcagcttgc aagatcccct
     4741 gcataaattt cgcgcgggca gggggcgatt ggcacgtgtc ggctatggct cgcgctttgt
     4801 ggatgcactt ttctccctga cgctgatgct gcgcggccgg gtcccaggag atacggctgc
     4861 tgcagcagcg ctcgcttata agcgcatgca agatgagact gagcgctact gttattatgg
     4921 tcgtgtcgct cgtcagaacg gttgggtgat attgcagtat tggttctttt atcctttcaa
     4981 taactggcgt tctggctttt ttggcgtaaa cgaccacgag ggcgactggg aaatgattag
     5041 catttattgc tcggagaccc actctgatgg cgcagagttg ccatcagttg accgcttggt
     5101 gccagagtgg gtggcatatg cgtcccacga tttctctggc gacgatttgc gccggcgctg
     5161 ggatgatcca gagatattga aggtacccga cgcccagggc aatctacacc cggtagtttt
     5221 tgctggggcg ggcagtcatg ccagttattt tagccagggt gagtatttaa cggagcttga
     5281 acttcctttt ctctctgcat tggttcaact ggtggataag ttacaaagtt tgtgggtgaa
     5341 ggtcctacac caatcgagtg gcagtgcatc atcgcctggt tttaatgtct ttagaatccc
     5401 attcgtggat tatgcccgcg gtgatggttt ggcgcttggc cctgggcagg atcgagagtg
     5461 gcagccagag gtgataagcg agagcacagc ctgggctatt tcttatcggg gactttgggg
     5521 tttatacgca agggatccaa ttgcttgtga aaacgcaccg gcggggccgg tgtataatcg
     5581 cgatggaagc gtaaggcaga gctggtgcaa tcccctgggc tggtctggac tagataaatt
     5641 acccccgaac catgatgcgc cagaagtttt gaagcgcaag cttgatgaaa ttcaagccta
     5701 tcaggtgagc ctaaaaacca aaattgacca gcagagttcc aagctagtgg ggatgggggt
     5761 tgaagcggcg gctatgcagg gttatcctca tctagagcaa gcacaccgcg cccaagaaga
     5821 gcagatagcg acgttgtcag cagaagtgca tcatctacgc caggagctgg ctcaggtgga
     5881 tgcccgactg gatgccctca ggctgtatgg tgagcagtta agccaggctg atcccggccc
     5941 gatgcgggct catattcacc gtgctcatca cccttcgcct gaaagtgagc tgcgactggc
     6001 cagtgtggca gagtttgtcg ctgcagtgag catcggtgca ttgattgtcg gtattgtgct
     6061 tttgattgtg tt
//
LOCUS       DBNGSX010000395        13145 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1698036, whole genome shotgun sequence.
ACCESSION   DBNGSX010000395 DBNGSX010000000
VERSION     DBNGSX010000395.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13145)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13145)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13145
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1698036"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..260
                     /gene="iscX"
                     /locus_tag="AC16CB_11760"
     CDS             42..260
                     /gene="iscX"
                     /locus_tag="AC16CB_11760"
                     /inference="COORDINATES: protein motif:HMM:NF016284.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HIR3343191.1"
                     /translation="MPLDLTLTWEDTYAIALLLIERHPEMRVEDISLGMIYRWTTSLP
                     EFSDDPELANDAILTAIYQAWYEEVNPL"
     gene            257..916
                     /locus_tag="AC16CB_11765"
     CDS             257..916
                     /locus_tag="AC16CB_11765"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HIR3343192.1"
                     /translation="MSEELNTQGYDIPPELQGQVAITTLDKIYNWGRRSSIWPMMFGL
                     ACCAIEMICTAASRYDLARFGMEVMRPSPRQADMMIVSGTVTKKMITTIVRLYNQMPE
                     PKYVVAMGACASGGGPFKEGYTVVSGVDNYIPVDVYIPGCPPTPQALLNGLITLQKKI
                     DTQSIKQVRWYQKEGNEMVPIPVLGPDIMDPRQYDLIKSYAQEKLERASDAASQAEET
                     A"
     gene            931..1311
                     /locus_tag="AC16CB_11770"
                     /pseudo
     CDS             931..1311
                     /locus_tag="AC16CB_11770"
                     /inference="COORDINATES: protein motif:HMM:NF012549.8"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
     gene            1570..2673
                     /locus_tag="AC16CB_11775"
     CDS             1570..2673
                     /locus_tag="AC16CB_11775"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HIR3343193.1"
                     /translation="MKTDQVVVNLGPQHPSTHGVFRIVVTLDGETVLSLKPVMGYLHR
                     NHEKIGERNTFLHNMPFTDRLDYLSSMSNNFGYALAVEKLMGIKPPERAEYLRVIMAE
                     LTRISNHVWAIGFLLNDLGAYFTPALYFIQERELILDIFEAVSGSRMMCNYFRFGGVA
                     RDLPEGVYEKIREIVYERLPRKVDEMDTYLTNSEIIRTRGEGVGVLTPEQAIAYATCG
                     PVLRASGVPYDVRRADPYSIYDRFEFDVAVRYHGDVYDRYLIRMDEIRQSLRILQQAL
                     RDLPEGSIQEGKPQYQVRVPAGESYGRVEGPKGELGYYVISNGKPNPWRYHVRAPSFI
                     NLTAMETMCIGNKIADVVAILGSIDIVLGETDR"
     gene            2687..3295
                     /locus_tag="AC16CB_11780"
     CDS             2687..3295
                     /locus_tag="AC16CB_11780"
                     /inference="COORDINATES: protein motif:HMM:NF024247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR3343194.1"
                     /translation="MDGNGILKGLGVTLRRFVDTYLEDLRWWGKRYYTQEGIAHRQSN
                     KTRGIFTVQYPEEKLPVPEEFRYIPFLLYEEGTNGDIVERCTSCGICAKVCPPQCIWI
                     VRTSDPNTGRPVPEPKEFYIDVDICMNCGFCSEYCPFDAIKMDHDYELAVYDRHQHNI
                     FDKDKLMKPVSYYASIRPTNYNREEEARAAAEAAKAARKAQA"
     gene            3339..4421
                     /locus_tag="AC16CB_11785"
     CDS             3339..4421
                     /locus_tag="AC16CB_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HIR3343195.1"
                     /translation="MGKSVVTEAAVLEALGKVQEPELHRDLVSLNMIRDLKIDGDRVS
                     FTVMLTTPACPMRSQIEQDARSAVATLPGVSEVDIDMSANVPSDGRPRGLLDLPIRNA
                     VAIASGKGGVGKTTVAVNAAVVLAQSGARVGLLDADIYGPNVPTMMGLQKLPAAQADK
                     LQPAEAFGVKVMSIGFLVKPDQPLIWRGPMLHSAIRQFLTDVNWGELDYLIIDLPPGT
                     GDAGLSLAQSLPLSGGVIVTLPQQVSLDDARRGLEMFRQLDVPIFGVVENMSYLELPD
                     GNRVDVFGSGGGERLAQESGVPFIGAIPMDPSVRAGGDNGTPVTISHPESAVASALTR
                     VAMDIAAKISVAAMQQGSFIPINLIG"
     gene            4508..5455
                     /locus_tag="AC16CB_11790"
     CDS             4508..5455
                     /locus_tag="AC16CB_11790"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSP1 domain-containing protein"
                     /protein_id="HIR3343196.1"
                     /translation="MAPIIVGIRFQKIGKVYHFDASRFPDLQVGDQAVVETSRGAQLG
                     EVVQILEDPQPPPEGNWKSIQRRATPRDLVLRQIWQKKELEVTINCRAKAAEVKLDGV
                     KIVGADFSFDGSRLSILYSTEAETKIDTKRLRSAMQRMYPRSRVEMRLIGPRDVAKIL
                     GGMGACGMVNRCCSMFLTEFSPISIKMAKEQGISLTPSEITGMCGRLRCCLVYEYEQY
                     VEARKQLPKRNKRVVTPQGEGKVLDIYPLKESVLVELDRGFQQEFPQAELQPWDELEA
                     LRRKSQSPCDKHEGGQCDCGKEKKEPTQPRKMGRGHKNR"
     gene            5545..6690
                     /locus_tag="AC16CB_11795"
     CDS             5545..6690
                     /locus_tag="AC16CB_11795"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 metallopeptidase family protein"
                     /protein_id="HIR3343197.1"
                     /translation="MFEYTRSLRRDFHRHPELGFQEVRTAGIVAKELAELGLEVVSGV
                     GKTGVVALLEGKRPGPVVLVRFDMDALPIVEESEVEYASQNLGVMHACGHDGHTAVGL
                     TVARLLQAHSQELAGTVKFVFQPAEEGLGGALAMVADGVLEIPRPDFALALHLWNEKP
                     IGWLGISPGPVMAASEIFKVRVIGKGGHGASPHLTVDPVLAGAHIVTALQGIVARNVP
                     PLESAVVSVTTFRSGESFNVIPQVVEMMGTIRTFVPSVRQLVLERFRQVVEGVAAAMD
                     CRAEVEIASITPAVVNDLSLTQGVQETARRLWPNNPLDLDYRSMVSEDMAFMMQDIPG
                     CFMFIGTANVEKGLNAPHHHPSFNIDEDALPRAAALMSAAVAAHMGA"
     gene            6773..7675
                     /locus_tag="AC16CB_11800"
     CDS             6773..7675
                     /locus_tag="AC16CB_11800"
                     /inference="COORDINATES: protein motif:HMM:NF013648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HIR3343198.1"
                     /translation="MDNHPADKENGLWLSRHFENPPKRVVSLVPSLTESLFELGFGEV
                     VVGVTDYCVHPSEHLVDLPRLGGPKNPRVDEIIALQPELILVNWEENTRRTVEELEAA
                     GILVWVTFPKTVREALDLLWILAGVFHSRLAAARVESLEVALDWAQQAAQQRAPLRYF
                     CPIWYEKTSAGLPWWMTFNRHTYPHDLLLTLGGENAFSDRERRYPLEADLGLAEPQDS
                     DGWDTRYPRLSLEEIRLSNPELILLPNEPYAFTNNDRDELIAQLADTQAVQGGRVYPI
                     DGSLITWHGTRLARALQELPILFE"
     gene            7774..8478
                     /locus_tag="AC16CB_11805"
     CDS             7774..8478
                     /locus_tag="AC16CB_11805"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR3343199.1"
                     /translation="MTRTRPPSEFLQFLISLPNGEEDSGRVPSLTELSSKLEISVARL
                     REQLEVAKALGLVDVRPRTGIRRKAYAFFPAVWQSLGYAVELDHAYFDAFAELRNHLE
                     AAYWHQAVRLLTPQDHLELQRLVANALEKLQGPQIQIPHSEHRQLHICIYSRLQNPFV
                     LGILEAYWQAYEAVGLNLYAGYDYLQEVWEYHRRMVDAICCGDFDGGYQALVEHTDLL
                     HHRPLSVLMGNSITEK"
     gene            8502..10358
                     /locus_tag="AC16CB_11810"
     CDS             8502..10358
                     /locus_tag="AC16CB_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR3343200.1"
                     /translation="MTALTTLAEEESRAETEDRINNFSITVATVNGSGSQTSNLTILR
                     ALFKMGIPTSGKNLFPSNIQGLPTWYTIRVSKDGFLARREEHEIVVAMNPATFSRDLA
                     DVSPGGVFFYADDIKMSVNRDDIVSYAMPVKQIVRESGAPSSLRDYVANMVYVGILAQ
                     MLGIDLDKIYQAISFHFKGKTKPIEMNWEVVQASANWAAQNLEKTDPFGVEPMDATTD
                     YIMADGNTAAALGSIFGGVQFVSWYPITPASSLAESLAEYLHLLRKDPETGKNTFGVV
                     QAEDELAAIGMAIGAGWSGLRAMTSTSGPGLSLMTEYAGLGYYSEVPIVVWDVQRVGP
                     STGLPTRTMQGDLTFVYFMGHGDTNQVILLPGTVSECFEFGWKSFDIAERLQTPVFVL
                     SDLDLGMNNWMTRPFQYPDKPMDRGKVLWEEDLKKLNGQWGRYKDVDGDGIPYRTLPG
                     NRHPQAAYMTRGTGHDEYAHYTEDPDEWHNMMERLKRKFETARKYVPAPIFDLMDGAE
                     IGLIGFGSTDAAILEARHQLHKTRGIKTDFMRVRALPFPDEVGEFIRSHPRNYVVELN
                     RDGQMHQLLSLDYPDVVVKLHSLAYHDGLPLTAARVRNMVLEKEDLHEQEDR"
     gene            10360..11403
                     /locus_tag="AC16CB_11815"
     CDS             10360..11403
                     /locus_tag="AC16CB_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HIR3343201.1"
                     /translation="MTAQKTAPAPNVNLIGLSKADYRGGQSTLCQGCGHNSISSQIIA
                     ACYELDILPEDIVKFSGIGCSSKSPTYFLNRSYGFNGLHGRMPSLATGAMFGDHSLHG
                     LGVSGDGDSASIGMGQFKHILRRNLRMVYIVENNGVYGLTKGQFSATAEVGLTLKSQG
                     TNPYMPVDIAWEALVGNATFVGRSFAGDPKQVKELIKAALSHNGIAVLDIISPCVTFN
                     NQDSAMHSYSWGREHEVALHELSFVPPAEEIMVDYRTGEIIEVTMHDGSSVILKKLEH
                     DYDPTDRSEAMRLLEEANRKNWLLTGLIYIDTNQKSLLDLYNLPETPLNRLTESRLRP
                     GPETIKKVNDLMF"
     gene            complement(11576..12322)
                     /locus_tag="AC16CB_11820"
     CDS             complement(11576..12322)
                     /locus_tag="AC16CB_11820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family NAD-dependent protein deacylase"
                     /protein_id="HIR3343202.1"
                     /translation="MVNVPEELIAIFRSARRVAVLTGSGISAESGVPTFRDAQTGLWA
                     RYRPEDLATPQAFRRDPGLVWEWYKWRRELVTKAKPNRGHEALVELEKYLAQRGCQFA
                     LITQNVDGLHQRAGSQQVIELHGNILRTKCFDEGTLVEKWEESDEKIPRCPQCGGLLR
                     PDVVWFGENLSAIALEAAWQATQSCDIFLCIGTSTIVEPAASLPFMALQYGASVIEIN
                     LNTTPLSTSVKYVFRGPAGIILPYLVDCIL"
     gene            complement(12486..>13145)
                     /locus_tag="AC16CB_11825"
     CDS             complement(12486..>13145)
                     /locus_tag="AC16CB_11825"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3343203.1"
                     /translation="ARIHVHPAGAPHLRDPEKLLNSAQRIYGDSMQTLWGEFLPVHPE
                     RLHIPQDGAVIEIGSLHFQVIDTPGHAEHHFVYVLDDLCFSGDIGGVRVGNLRHLRLP
                     MPPPELHLGKWRQSLMRLRELPIRRIAPTHYGIFNDADWHLAAIEKALNDVEAWIESI
                     MPSDPPIEILNQHFDEWLRQRSLNEGVQPGLLEEYKIVNPSWMSCYGIQRYWRKFRSE
                     N"
BASE COUNT         3128 a         3209 c         3618 g         3190 t
ORIGIN      
        1 ctgggttgac ggatctgcgt tctacgatat aattatgcgg aatgccatta gatctcacgc
       61 tcacctggga agatacttat gcgatcgcct tgctgttgat cgagcgccat ccagagatgc
      121 gtgtagagga catttcgcta ggtatgatct atcgctggac gactagtttg cctgaattta
      181 gcgatgatcc ggagctggct aatgatgcca tcctgaccgc aatttaccaa gcatggtatg
      241 aggaggtaaa ccccctatga gtgaagagct caacacccaa ggctacgata tcccgcctga
      301 attgcaggga caggtagcca ttaccaccct ggataaaatc tataactggg gacggcgttc
      361 ctccatctgg ccgatgatgt ttggcctggc gtgctgcgcg attgagatga tctgtactgc
      421 tgccagtcgc tatgatcttg ctcgcttcgg catggaggtc atgcgcccca gcccgcgcca
      481 ggcggacatg atgattgtct cgggcacagt gaccaagaaa atgatcacca ccatcgtgcg
      541 cttgtataac cagatgccag aacccaaata cgtggttgcc atgggtgcgt gcgcttctgg
      601 cggcggaccg tttaaagagg gctataccgt ggtctcagga gtggataact atatacccgt
      661 ggatgtatat attcctggct gcccacctac cccccaggcc ttgctgaatg gcttgatcac
      721 cttgcaaaag aaaatcgata cacagtcgat caagcaagtg cgctggtacc aaaaagaggg
      781 caacgagatg gtgccgatcc cagtgcttgg cccggatatc atggatccgc gccagtacga
      841 cctgatcaaa tcctatgccc aggagaagct cgaacgtgcc tccgatgcgg ctagccaggc
      901 tgaagaaact gcgtgaagtg gagactaacg atgagcgaac aagttcttac tgctaaagat
      961 acactggaaa cgcgtttccc agagattatc acccccgata ctcgcaaagg ttacgagggt
     1021 tacatcgtac aaaccgataa attggtagag gttgctacag ccctgcgcga cgagtttggt
     1081 tacgactatc tgtcctcggt cactggcgtg gattacttgc cagaaggcat actggaagcg
     1141 gtataccatt tctatcgaag cacgggtggc tctggactgg tgctcaaagc tcaaacgcca
     1201 cgtgataatc ccttgattcc gtctctggtg gaagtctacc ccggagttga gtaccaggaa
     1261 cgcgaggcgt gggatttgat gggcattcgt tttacgaatc accccgatct gcgccgcatc
     1321 ctcacctggg aggggtttca aggtcatcct ctgcaaaaag actggcgcga ggcttattac
     1381 gaggaggatg ggaaaccctt taagagccgc tggcccgatg ggcatgtcta tcggattgag
     1441 gacaagaacc catttaaaac gaatgtagat taccccgctg gttttgatcc cgagggttgg
     1501 gtcccggaag gggaatctgc cctgtacaac agcctggcga aatttgaata cacagattcc
     1561 gaatcaggca tgaagaccga ccaagtggtc gtaaatctgg gaccccaaca cccatccacg
     1621 cacggcgttt tccgcatagt ggttacactc gatggcgaga cggtgctcag cctcaagcca
     1681 gtgatgggat atctgcatcg taatcatgag aagatcggtg agcgcaatac atttctgcat
     1741 aacatgccat tcaccgaccg cttagactac ctctcttcaa tgagcaataa cttcggttat
     1801 gctttggctg tagagaagtt gatggggatt aaaccccctg agcgggcaga atacctgcgc
     1861 gtcatcatgg cagagttgac ccgtatcagc aaccatgtct gggcgattgg ctttttacta
     1921 aatgacctgg gcgcgtactt cacccccgct ctctacttta ttcaggaacg agagctaatc
     1981 ttagatatct tcgaggcggt ctctggctcg cgcatgatgt gcaattactt ccgcttcggt
     2041 ggcgtggcgc gtgacctgcc tgaaggcgtc tatgagaaaa tccgtgagat agtgtatgag
     2101 cgcttgccgc gcaaggtaga tgagatggat acctatctta caaacagcga gatcatccgc
     2161 acgcgtggcg aaggagtggg cgtccttacc cctgaacaag ccattgctta tgcaacatgt
     2221 ggcccagtgc tgcgtgcttc aggtgttcct tatgatgtgc gccgggctga tccttacagc
     2281 atctatgacc gcttcgaatt tgatgtggct gtccgctacc atggcgatgt ttacgatcgc
     2341 tacctgatac gcatggatga gattcgccaa agcttgcgca tcctgcaaca ggcgctgcgc
     2401 gacctgcccg aagggtcgat ccaggaaggc aaaccacagt atcaggtgcg cgtaccggct
     2461 ggtgaatctt acgggcgcgt agagggtccg aaaggtgagc tgggttatta cgttatttcc
     2521 aacggaaagc ctaacccgtg gcgatatcat gtgcgcgccc cttctttcat caacttaacg
     2581 gcaatggaga ccatgtgtat aggcaacaag attgccgatg tggtagcaat cctgggctcg
     2641 atagatatcg tcctgggtga aacagatcgg tgacattgga gatattatgg acggaaatgg
     2701 aattttaaaa ggattaggcg tcaccctgag gcgcttcgtg gacacatacc tggaagattt
     2761 gcgctggtgg ggtaagcgtt actacaccca agagggcata gcccatcgcc agagcaacaa
     2821 gacgcgcggc attttcacag ttcagtatcc cgaagagaag ctgccagtgc cagaagagtt
     2881 tcgctatatt cccttcttgc tctatgaaga gggcacaaat ggcgatatcg ttgaacgctg
     2941 cacttcttgt ggtatttgcg ctaaggtttg ccctccgcag tgtatctgga ttgtgcgcac
     3001 gtctgacccc aataccgggc gtccagttcc agagcccaaa gagttctaca tcgacgtgga
     3061 tatttgcatg aactgtggat tttgttctga gtattgtccc tttgatgcga tcaaaatgga
     3121 tcacgattac gaattagcgg tgtacgatcg ccaccagcat aatattttcg ataaagataa
     3181 attgatgaag ccggtgtctt attatgcctc catccgccca acgaactata accgcgagga
     3241 agaggcacgt gcagccgccg aggcagcgaa agcagctcgc aaggcacaag catagttgat
     3301 ggctgcacgc agatcgacta gtttatagga tggatattat gggaaaaagt gttgtgactg
     3361 aggcggcggt tttggaggcg ctcggcaaag ttcaagagcc ggagctacac cgcgacttgg
     3421 tctctttgaa tatgatccgc gatttgaaaa ttgacgggga cagggttagt tttactgtta
     3481 tgttgacgac acctgcctgt ccgatgagat cccagataga gcaggatgcg cgttcggcag
     3541 ttgcgacttt gcctggcgtg agcgaggtag acatagatat gtctgccaat gtgcccagcg
     3601 atggacggcc gcgcgggctg ctggatctgc ccatccgcaa tgcagtggca attgcctcgg
     3661 gcaaaggcgg ggtaggaaag accacggtag ctgtcaacgc agccgtagtc ctggcgcaga
     3721 gcggcgcccg tgtgggattg ctggacgcag atatctatgg cccgaacgtg cccacgatga
     3781 tgggcttgca gaagctgccc gctgcgcaag ccgacaagct acagcccgct gaggcgtttg
     3841 gtgtcaaggt gatgtcaatc ggttttctgg tcaagcccga ccagccgcta atctggcgtg
     3901 gacccatgct gcactccgct atccgccaat tcctcaccga tgtcaattgg ggcgagctgg
     3961 attacctgat catcgacttg cctccaggga ctggtgatgc tggattgagc ttggcccagt
     4021 ccttgccatt gagcggaggc gtcatcgtta ccttgcccca acaggtatca ctggatgacg
     4081 ctcgccgtgg cttggagatg ttccgccagt tagatgttcc catctttggc gttgtcgaga
     4141 acatgagcta tttggagctt cccgatggca accgggtaga tgtctttggg agcggtggag
     4201 gtgagcggct ggcgcaagag tcgggcgtgc cttttattgg ggctattcct atggatccgt
     4261 cagtgcgtgc tggaggcgac aatggcacgc cagtgactat ctcccaccct gaatctgcgg
     4321 ttgcaagcgc cctcacccgt gtggcgatgg atattgctgc taaaatcagt gtggctgcca
     4381 tgcagcaagg aagctttatc ccgatcaatt taatcggtta gattatttca tgttgcagga
     4441 ttgtagttgt cagttggcgg caaccggttt atactagccg tcaactgttc tatagtggct
     4501 aattattatg gctcccatta ttgtcggtat acgttttcaa aaaattggta aggtctacca
     4561 cttcgatgcc agcagatttc cagacttgca ggttggcgac caggcggtgg tggagaccag
     4621 tcgcggcgcc caactgggtg aggtagtaca gatcctggaa gatccacagc ctccacccga
     4681 aggcaactgg aaatctatcc agcgccgcgc aactccgcgc gatctggtat tgcgccagat
     4741 ttggcaaaag aaagagctgg aagtcacaat caactgccgt gcaaaggcag ctgaggtaaa
     4801 gcttgatggc gtcaagatcg ttggggctga tttctcgttc gatggatcac gcttatccat
     4861 tctatatagc actgaggcgg aaactaagat cgatacaaag aggctgcgtt ccgccatgca
     4921 gcgcatgtac ccgcgctccc gtgtggaaat gcgtctcatc ggtcctcgag atgtagccaa
     4981 gatcctgggt ggcatggggg cttgcggtat ggtaaaccgt tgttgctcga tgttcctgac
     5041 cgagttcagc cctatatcga taaagatggc aaaggagcag ggtatctccc tcaccccatc
     5101 ggagattaca ggtatgtgcg ggcggttgcg ttgctgtctg gtgtatgaat atgagcagta
     5161 tgtggaagcc cgcaaacaac tccccaaacg caataagcgt gtggttacac cccaaggtga
     5221 ggggaaggtg ttggatatct acccgctcaa ggagtctgtc ttggttgagt tggatcgagg
     5281 gtttcagcag gaatttccgc aggctgagct tcaaccctgg gatgaattgg aagctctgcg
     5341 tagaaaatcg cagtcaccct gtgataaaca cgagggcggg cagtgcgatt gcggcaagga
     5401 gaaaaaggag cccacccaac ctcgcaagat gggtcgtggc cacaaaaacc gttaatccga
     5461 aaaggaagac cctaaaagca agcaagcttt tagggtctag ttttttttat gtccatgagt
     5521 gattttctta aagaagcgga aaatttgttt gaatataccc gatcccttcg gcgtgatttt
     5581 caccgtcacc ccgagctggg ttttcaagaa gtgcgcacag cagggatcgt tgctaaagag
     5641 ctggcagaac tagggttgga agtcgtctct ggggttggca agaccggtgt agtcgccctg
     5701 ctggagggta agcgccccgg cccggtggtt ctggtgcgct ttgatatgga tgcactgcca
     5761 atcgttgaag agagcgaggt ggaatacgcc tcacagaact tgggtgttat gcatgcttgt
     5821 ggtcatgatg ggcatactgc tgttggtcta accgtggcgc gtctgctgca agcccacagc
     5881 caggagttgg caggtactgt caaattcgtg tttcaacccg ccgaggaggg gttgggtggg
     5941 gctctggcaa tggtcgcgga tggcgtgctg gagattccga gacctgattt cgctctagcg
     6001 ttgcaccttt ggaacgagaa gcccatcggc tggctgggga ttagccctgg tcctgtgatg
     6061 gctgcttcag aaattttcaa agtgcgggtc attggaaaag gtgggcatgg ggcttcacct
     6121 cacctgacag ttgaccctgt gttggctggg gcgcatatcg tcaccgcatt acaggggatc
     6181 gtcgctcgca atgtcccgcc gcttgagtcg gctgtggtca gtgtaactac ttttcgtagc
     6241 ggagagtcat tcaacgtaat tccgcaggtc gtggagatga tggggacaat ccgcacgttt
     6301 gtgccttcgg tgcgccagct ggtgctggaa cgtttccggc aggtggtgga aggtgtagct
     6361 gctgcaatgg attgtcgggc tgaagtggag atcgcgtcga ttacgccggc ggtggtcaac
     6421 gatttgagtt taactcaggg tgtccaagaa actgctcgcc gattgtggcc aaataacccc
     6481 ttggatctcg actatagatc gatggtctcg gaggatatgg ctttcatgat gcaagatatt
     6541 cctggttgtt tcatgtttat cggaactgcc aatgtcgaaa aaggcttgaa cgcgcctcac
     6601 caccacccga gtttcaatat tgacgaggac gcacttccac gcgctgcagc gttgatgtcc
     6661 gcggctgttg cagctcatat gggagcatga agcttgtgtc aagccctcag gcaaaaggac
     6721 tttagacaag cagcgtatat ggtgagttgt ggtgataaac actggtggag acatggacaa
     6781 tcatcctgct gataaggaaa atggcctttg gctcagcagg cattttgaaa accctccgaa
     6841 gagggtagtt tcattagtgc catctttgac ggaaagcctg ttcgagctgg gctttggcga
     6901 ggtggtagtg ggtgttacgg attactgtgt gcacccatct gagcatttgg tagatctgcc
     6961 acgcttgggt ggtccaaaaa acccccgagt ggacgagatt atcgcgttgc agcctgaatt
     7021 aatccttgtg aactgggagg agaacactcg ccggacggtc gaagagctgg aagcggctgg
     7081 catcctggtt tgggtgacgt ttcctaagac ggtccgcgag gcattggatt tactttggat
     7141 actggcgggc gtgtttcaca gcagattggc ggctgcacgg gtggaaagcc tggaagtcgc
     7201 tctggattgg gcccagcaag ccgcccaaca gcgagcacct cttcgctatt tttgtcccat
     7261 ctggtacgaa aagacaagcg caggcttgcc atggtggatg acattcaatc gccataccta
     7321 tccacatgac ttgcttctga cacttggggg agaaaatgct ttttcggacc gtgaacggcg
     7381 ctaccctctc gaggcagact tgggcttagc tgaaccacaa gattctgacg ggtgggacac
     7441 gcggtatccg aggctaagtt tggaagagat ccgtctttcc aatcctgagc tgatcttgct
     7501 tcctaacgag ccatacgctt ttacaaacaa cgaccgtgat gaattaatcg cccaattggc
     7561 agatacccag gcagtgcaag gcgggagagt atatcctata gatggcagct tgatcacctg
     7621 gcatggtaca cggctggcta gggcactcca agagctcccc attctcttcg aatagcctgt
     7681 gttgcttgac tcgcgttaga tagcatgata agattctcat tagttataat catcagatta
     7741 atgcgtataa tatgaataat ctacaggtat gctatgaccc gcacacgccc accttctgaa
     7801 tttttacagt tcctgatatc tctccctaat ggagaagagg actcggggcg ggttccttct
     7861 ttgactgagc tcagttcaaa attggagatc agcgtggcca ggttgcgcga gcaactggaa
     7921 gttgccaagg cgttgggatt ggtggatgtg aggccacgta ctggtatccg ccgcaaagct
     7981 tacgcctttt ttcccgccgt atggcagagc ctgggctacg ctgttgagct agatcatgcc
     8041 tactttgatg cctttgccga gctacgtaat cacctggaag ctgcttactg gcaccaggct
     8101 gtccgtttgc ttacgccgca agatcacctt gagcttcaaa ggctggtggc aaacgctttg
     8161 gaaaaattac aaggccctca aatccagatc cctcacagcg aacaccgcca gttacatata
     8221 tgtatctata gcaggctgca aaatcctttt gtgctgggta ttttggaagc ctactggcag
     8281 gcatatgaag ctgttggttt aaatctttac gctggctatg attacctgca agaagtatgg
     8341 gaatatcatc gacgcatggt ggatgcgata tgctgcggtg attttgatgg cggctatcag
     8401 gctctagtgg agcatacaga cctattacac catcgcccgt tatcggtgtt gatgggaaat
     8461 tcaatcacag aaaagtaatt agttcatctc aaggagtagt gatgactgca ttaactacgc
     8521 ttgccgaaga ggaatcccgt gctgagaccg aggatcggat aaataatttc tcgatcaccg
     8581 ttgccacggt caatggttcg ggaagccaaa cttccaatct cactattttg cgcgcccttt
     8641 ttaagatggg tatcccaact tcagggaaaa atcttttccc atcgaacatt cagggcttac
     8701 cgacctggta caccatccgc gtgagcaagg atggctttct ggcccggagg gaagagcatg
     8761 agatcgttgt ggccatgaac ccggccacct tttccagaga tttggccgat gtttcaccag
     8821 ggggcgtctt tttctatgcc gatgacatca agatgtcggt caaccgagac gacattgtgt
     8881 cgtacgcaat gccggtaaaa caaattgtcc gggagtctgg cgctccttca agcttgaggg
     8941 attatgtcgc taacatggtt tatgtgggca ttttggcgca aatgctgggc attgatctgg
     9001 ataagatcta tcaagcaatc agctttcatt ttaaaggcaa aactaaaccc attgaaatga
     9061 actgggaggt ggttcaggcg tcggccaatt gggcggctca gaatctagaa aagacggatc
     9121 ccttcggcgt tgagccaatg gatgccacaa cagactacat tatggctgat ggaaacaccg
     9181 ccgctgcttt aggctctatc tttggagggg tgcagttcgt ctcctggtat ccgattaccc
     9241 ccgcatccag cctggcggag tcgctggcag agtaccttca cttactgcgc aaagacccgg
     9301 aaacgggtaa gaataccttt ggcgtcgtcc aggccgaaga tgaattagca gctatcggca
     9361 tggcgattgg ggcagggtgg tccggtttgc gagcgatgac gtccacctcc ggcccgggat
     9421 taagcttgat gaccgaatat gctggtctgg gatattattc ggaagtaccg atagtcgtat
     9481 gggatgtgca acgtgtgggt ccaagcactg gcttaccgac ccgtacaatg cagggcgacc
     9541 tgacctttgt atactttatg ggccacggtg atacaaacca ggttatctta ctacctggca
     9601 cggtgtcgga gtgtttcgag tttggatgga agtcctttga tatcgctgag cgcttgcaga
     9661 cacctgtctt tgtgctatct gatctggatt tgggtatgaa taattggatg acacgcccat
     9721 ttcagtaccc ggacaaaccc atggatcgag gcaaagttct ctgggaagaa gacctgaaga
     9781 aactgaacgg tcaatggggg cgctacaagg atgttgacgg tgatggtatc ccttaccgca
     9841 ccttacctgg taaccgtcat ccgcaggccg cctatatgac acgtggaacc ggacatgatg
     9901 agtacgccca ttacaccgaa gatccagacg aatggcataa catgatggag cggctgaagc
     9961 gcaaattcga gactgcccgc aaatatgtgc ccgctccgat ttttgatttg atggatggag
    10021 cggaaatagg actgatcggt ttcggctcca ccgatgctgc aattctcgaa gcccgccatc
    10081 aattgcataa gacgaggggc attaagaccg atttcatgcg cgtgagggcc ttgccctttc
    10141 cagatgaggt tggtgaattc atccggtccc acccacgtaa ctacgtggtt gaattgaacc
    10201 gtgacggtca aatgcatcag ctcctctctc tcgattatcc tgatgtcgtg gtcaaattgc
    10261 attcgctggc ttatcatgat ggcctacccc tgactgccgc tcgggtacgt aatatggttc
    10321 ttgaaaaaga agatttgcat gagcaggagg atagataaga tgactgccca gaagacagca
    10381 cccgcaccaa atgtaaacct gattggcttg agtaaagcag actaccgcgg tggtcagagt
    10441 acactgtgcc agggctgtgg tcacaactca atcagcagcc agattatcgc cgcttgttac
    10501 gagttggata tcctgccgga agatattgtt aaattcagcg gtattggctg ttccagcaaa
    10561 agccccacct attttctgaa ccgctcctac gggttcaatg gcttgcatgg gcgcatgcct
    10621 tctttagcga ccggtgccat gtttggcgat cattcgctac atggcctggg cgtaagtgga
    10681 gacggcgact ctgccagcat cggcatgggt cagttcaagc atatcctacg ccgcaatctg
    10741 cgcatggtgt acatcgttga gaataatggc gtttatggtc tgaccaaggg acaattctcg
    10801 gccactgccg aggtaggcct gacacttaag agtcagggca ccaaccccta tatgccggtg
    10861 gacattgcct gggaagctct ggttgggaac gccacctttg taggtcgctc ctttgcgggt
    10921 gatcccaagc aagttaaaga gctgatcaag gctgccttaa gccataacgg tatcgctgtt
    10981 ttggacatca tcagcccgtg tgtgaccttc aataaccagg actccgccat gcattcttac
    11041 agttggggca gagaacatga ggtcgctttg cacgagcttt cttttgtccc accagctgag
    11101 gagatcatgg tggactacag aactggcgag atcattgagg tgaccatgca tgatggttct
    11161 tcggtgatct tgaaaaagtt agagcatgac tacgatccca ccgaccggtc tgaagcgatg
    11221 cgtctattag aggaggcaaa ccgcaagaac tggctgctga ctggactgat ctatatcgat
    11281 acgaatcaaa agtcattgct cgatctctac aacctgcctg agacaccgct taatcgtctt
    11341 accgaaagcc gcctgcgccc tggaccagaa acgatcaaga aagttaatga cttgatgttt
    11401 taattcgctc catcaagtgt acaaaaaacc acacgtgagt aggtggacgg aaatacctgc
    11461 tcacgtgtta tttaatggca agatcgtaac ggaaaatagg gagattaggg tgagcgacgc
    11521 cagccatcat ccgtctccaa caggatatat cgtgcatctc ctagggagtc agaacctaaa
    11581 gaatacaatc taccaagtat ggcaaaataa tcccagcagg gccgcgaaaa acatatttca
    11641 cacttgtact taagggggta gtgttgaggt tgatttcaat aacgcttgcc ccgtattgca
    11701 acgccatgaa tggcagagat gccgctggtt ctacgatggt agaagtgcca atgcaaagaa
    11761 aaatatcaca ggattgggtt gcttgccagg cagcctccaa ggcaatggca gacaggttct
    11821 ctccaaacca taccacgtct ggcctgagca ggccaccaca ttgcgggcaa cgagggattt
    11881 tttcatcact ttcttcccat ttttcaacaa gtgtaccttc atcaaagcat ttggtgcgca
    11941 ggatattgcc atgaagctcg atcacttgtt ggctgccagc gcgctggtga agcccatcca
    12001 cgttttgggt gattaatgcg aattggcatc ctcgctgggc aaggtatttt tctagctcaa
    12061 ccaacgcctc gtgccctcga tttggttttg ctttcgtgac cagttctcgg cgccatttat
    12121 accattccca aaccaagcct ggatcacggc gaaaggcctg gggggtagct aggtcttcgg
    12181 gtcgatatcg agcccacaaa cctgtctgag catctcgaaa tgtaggtaca ccactttccg
    12241 cggagatacc tgatcccgtt aggacagcca cccgccgagc ggaacggaat atcgctatca
    12301 gctcttcggg tacatttacc acgatatctt caaaggccta gcaacattca aaatgcagcc
    12361 cgcaacgggg gcaattggtt tgtccccata ccaaatcctc aataggctcg ccgcaacgtg
    12421 ggcaggcatc caggtctgtt ggctcattga tccgctgatc ttcgagcgag gcatcaggaa
    12481 gcaactcaat tttcactgcg aaactttctc caataacgct gaatgccata acatgacatc
    12541 caagaggggt taacaatttt atactcctct aacaaccctg gctgcacccc ctcatttagt
    12601 gatcgctggc gtaaccactc gtcgaagtgc tggttaagga tttcaatggg cggatcggag
    12661 ggcatgatgg attcgatcca ggcttccacg tcattcagag ctttctcgat ggccgcgagg
    12721 tgccaatctg catcattaaa tatcccataa tgggtgggag caatgcgtcg gataggcagc
    12781 tcccgtaatc gcatcaagct ttgccgccac ttgcctaagt gtaattctgg gggtggcatt
    12841 ggcaggcgca ggtggcgcag atttccaacg cggacgccgc caatatctcc gctgaagcac
    12901 aaatcatcca gcacatatac aaaatggtgt tcagcatgac cgggtgtatc gatgacctga
    12961 aagtgcagac ttccgatctc gattacggcc ccatcctggg gaatatgcag gcgttctgga
    13021 tgaacgggaa gaaactcccc ccaaagcgtt tgcatcgagt caccgtagat gcgttgagca
    13081 ctattgagaa gtttctcggg atcgcgcaga tggggtgcgc cggctggatg cacatgaatg
    13141 cgggc
//
LOCUS       DBNGSX010000396         7381 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1074613, whole genome shotgun sequence.
ACCESSION   DBNGSX010000396 DBNGSX010000000
VERSION     DBNGSX010000396.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7381)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7381)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7381
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1074613"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            725..1891
                     /locus_tag="AC16CB_11830"
     CDS             725..1891
                     /locus_tag="AC16CB_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009456329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baeRF7 domain-containing protein"
                     /protein_id="HIR3343204.1"
                     /translation="MDILNRAELEQLMRKEQQWCVSIYLPTHRTGTDAQQDPIRLKNL
                     LGKAEKDLSDRGMGRRDVRKMLEPASVLLQDFDFWQHQSDGLAIFLSSNGIRRYRLPL
                     NLEEFVTVMDRFHIKPLLPLFTGDGQFYILALSQNEVRLLLGTRYSVSQIDIGQVGGS
                     LAEAIPSTNHQMSLQLHSSGSTGGMSGAGSATFHGQGGGSDGSDKNELLRYFRLVSDG
                     LTEFLQGDRVPLVLAGVEFLLPIYKEANTYPNLIDTVITGNPDLLSADELHKSAWDIV
                     SPHFQTAREEAVAHYRQLAGQVSEQVADTLKKIVPAASDGRIETLFIAAGVQHWGIFN
                     PDTNEIDLHDQMESGDESLLDLAAVQTYLKGGTVYAVEPELVPGGTSAAAVLRY"
     gene            complement(1892..2083)
                     /locus_tag="AC16CB_11835"
     CDS             complement(1892..2083)
                     /locus_tag="AC16CB_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343205.1"
                     /translation="MLGDRYVRHSAIVEVESDTDKSIEQRKASGVRKFPAHTQAGKRQ
                     INPECAATKRAVTRLSNDI"
     gene            2516..3097
                     /locus_tag="AC16CB_11840"
     CDS             2516..3097
                     /locus_tag="AC16CB_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HIR3343206.1"
                     /translation="MPTFNLNDVRKLDPGFHRQQLNYWHNQGYIKSLAGGYYILADRV
                     MDEMVLFMVANKIYEPSYVSLESALAYYEIIPETVLGVTSISSRKTKQYESAWGVFSY
                     RSVKPQTMIGYQVIENTPAIKFKIAYLEKALLDYLYLNSEIQSLADFEELRWNRTQLR
                     GLLDHSIFAKYVKLFDKRALKNRVNQFLEYLNA"
     gene            3090..3869
                     /locus_tag="AC16CB_11845"
     CDS             3090..3869
                     /locus_tag="AC16CB_11845"
                     /inference="COORDINATES: protein motif:HMM:NF020419.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HIR3343207.1"
                     /translation="MLDLQQIKNEYSEPLQGYERAILREYLQYKILQAIFESKQASML
                     SFLGGTALRIVHGNSRFSEDIDLDNFGLSSQAFGELIQKVKLFLELEGFLVETSMVAK
                     GAFHCDLRFPELLYKQGLSPLHQEKILIQLDTIAQGYTYAPEVKILNKFDVFTEIRVT
                     PLNVLLSQKIYTAINRKRPKGRDFYDITYLFSKTRPDFGFIKQKMGITSPQSLREELL
                     LKIGDIDFDALAGDVAPFLITKEQVMRVVKFREFWKQVDLD"
     gene            4533..7235
                     /locus_tag="AC16CB_11850"
     CDS             4533..7235
                     /locus_tag="AC16CB_11850"
                     /inference="COORDINATES: protein motif:HMM:NF037660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3343208.1"
                     /translation="MQKADTLIRTKLRLPFTRPELVPRLHLQEQIAQGLRGPLTLVTA
                     PAGFGKTTLIASYITNCGLPIAWLSLDKDDNQAGRFLNYLVAALQEADYTIGNEAAQL
                     VAAAQQAPPEAILTSLINDLDTSEGEIVLVLDDYQFIKNQVVHDVVTFLLEHCPYTFH
                     LLIATRSDPPLPITRLRARGQTIELRTADLSFTEVEAAVFLNDVMGLNLDAGSVAALA
                     ERTEGWVAGLQMAALSMRDREDVRGFIEGFSGTNRYILDYLMEEVLASQPPEIQRFLL
                     YTSILERLTAPLCDAVTEIQEKMDSSNESIPSSRDSQSILEYLDRANLFLIPLDDARV
                     WYRYHHLFADLLRARLHQTHPDLIHHLHMRASAWLEEKGIISEAIHHLFAIQEIGRAA
                     DLIERYGPVYLAQSDPSIFQMADSLPAEMLIARPKIGIYLAWFLIVQGHTRKAVQILH
                     DVRRQLADLDSGQQWMQTFIATALAFLAPPGSNPDVYPLPDFRLLDEIPADEMVLRNA
                     ADFLYGMALARRGELHRAVEVSLECIQRERLQRGTLTIPTLAPFLSRFYLMLGRLHAS
                     ANLCREFLDPIQERGIRFVYTAGSMKIDLGEVLYEWNCLDEAELHIRDGLRDNEPWRN
                     IMTDGFGLVALTRLLQSKGKYAEAMQVVEKFEKRLREDSQPREFDEDLRTLRVRVELF
                     SGDLQNPSLWADQILLSEDFELHEEYYRLTLARIRLAQGRYAEVEKILAGMTPQITAG
                     SRITRQLECNLLLALAVAGQQRLPEAFKFIESTLALAEPEGYMRIFLDVGEPVRELLT
                     AYLRSDAPAHKIYARNILDAFSLTRQTSSPGPQPAGLIEPLSGRELEVLELLALGKSN
                     REIAGQLFVSPGTVKAHTSSIYRKLDVANRTEAVARARQLDILP"
BASE COUNT         1820 a         1824 c         1899 g         1838 t
ORIGIN      
        1 ctttcacaaa gtagcttgac gcacccaagt ttctatggcg cgcaaatcgc aatcgctgaa
       61 aacaagtgcc gccttgcgta agcatttctg ctgtggcgta tgaaatttac cgatttttct
      121 cgatatcgca cgaaaagcaa gttgtctgac tttttcttgt gaaagccctg cctgaccgat
      181 ggatgtgtct tggggcctgt ggttgtatgt tgtataatga attatgcctc tttctaaatc
      241 tcatcaccgc ctcatccagt ccgttaatga ttttatagaa tgagaaactg tttgattggt
      301 aatccgatga acacaaccag gcggcctatt gtgacgccat cgagattctg caccgcttta
      361 cataaagttg ggtagttcca gcctggctat aacagtaacc ccaagaggtt taagtcttct
      421 tgttgaaacg gtcaagcgaa gggtttaatc tagaaatagg aaatggagtc tccagggtga
      481 gtatgacgaa gtcgagacaa tgtgtcgcaa gtataccgag caggctgacg acgacggcga
      541 gcatgccgcg gccagccgtt tcgccgaaat gcacggcgac gaaatgggtt accccgacac
      601 cgcggcgatt gagtcaagcg accctggaca gcccgacggc aaagggcgcg gtgagccccg
      661 cggtgaggac gtggtcgaag gcgagtatca ggaaatgtag agtatttaaa ccaggagaaa
      721 gaaaatggat attttgaata gggcagaatt agaacagtta atgcgtaaag aacagcaatg
      781 gtgtgtttca atctatctgc caacccatcg aaccgggaca gatgctcagc aggatccgat
      841 tcggctgaaa aacctgctcg gcaaggctga aaaagacctg tcggaccggg gcatgggcag
      901 gcgtgatgtg cgaaaaatgc tcgagccagc cagtgtactc ctgcaagatt ttgatttctg
      961 gcaacatcag agtgacggac tggcgatttt tctttcttcc aacggaattc gccgttatcg
     1021 actcccgttg aaccttgaag aatttgtaac agtcatggac cgcttccaca tcaaaccgct
     1081 gctgcccctc ttcactggcg acggccaatt ctacatcctg gcgttgagcc agaacgaagt
     1141 ccggttgctg ctgggtacgc gctacagcgt cagccagatt gacattgggc aggttggtgg
     1201 cagtctggcc gaggccatcc catctaccaa tcatcaaatg agtctgcaac tccattcttc
     1261 cggttccaca ggggggatgt ccggggccgg ttcagcaaca ttccacgggc aaggtggtgg
     1321 cagtgatgga agcgacaaga atgagttact gcgctacttt cgcctggttt cggatggctt
     1381 aacggaattt cttcaggggg accgggtacc gctcgtgttg gcgggcgttg aattcctgct
     1441 tccaatctac aaggaagcca acacctatcc gaatttgata gacacagtca tcacgggaaa
     1501 ccctgatttg ctgagcgccg atgagttgca caaaagcgcc tgggacattg ttagcccgca
     1561 cttccagaca gcacgagaag aggctgtcgc ccattaccgg caacttgctg gtcaggtaag
     1621 cgaacaagtc gcagacacat tgaaaaaaat cgtcccggcc gcttctgacg gccggattga
     1681 aacattgttc atcgccgccg gagtgcaaca ctggggtatc ttcaacccgg ataccaatga
     1741 gattgattta catgatcaaa tggagtctgg tgacgaatcc ctgctggact tggcggcggt
     1801 tcagacctac ctaaaaggtg gcacagtgta cgccgtggaa ccagaattgg tgccgggggg
     1861 gacatctgcc gctgctgtgc tccgttattg atcaaatgtc attgctcagc ctggtgacgg
     1921 cacgctttgt ggcggcgcat tcaggattga tctgcctttt tcccgcctgt gtgtgcgctg
     1981 gaaactttct tacaccgctg gccttacgct gttcaatgct cttatcggta tcgctctcga
     2041 cttctacaat agcagagtgt cttacatatc gatcgcctag catgaagatc tctgttaata
     2101 gtaagcctgc actcgtcgga atgaaggata gttaggggtt aaatcgcatt tccgttctaa
     2161 tatctcgtat tgctagaatt ggggtcatcg tttatcgttg agcgcgcagg atttgtgcgc
     2221 ggcgacttcg atgcaaatgt gggcgtagtc gagcgcagcg gctgacgctg aacgatacgg
     2281 cgaaagtgac tttggtggtc atggaatggc agataataca agctgctaca caggcgccag
     2341 agttgtacca cgcctacatc ggcgtggctc aaatgtggga ccagcaaaaa tgagccgctt
     2401 cttctacttg acaatagcac aatctatggt tattatattt cctaatgtat ataaatttat
     2461 tgacattcgg aaataaaaca tgagatttag agagtttgag tccaggatta gagctttgcc
     2521 caccttcaac ttgaatgatg tgcgcaagct cgatcccggt tttcacaggc agcaattaaa
     2581 ttactggcat aaccagggtt acatcaaatc actggctgga ggctattata tcttggcaga
     2641 tcgagtgatg gatgagatgg ttctattcat ggtggcgaac aagatttacg agccttccta
     2701 cgtcagcctg gagtcggcgt tagcttatta cgagataatt cccgaaaccg tgttaggagt
     2761 gacaagcatc agctcccgca agaccaagca gtatgaaagc gcctggggtg tattcagcta
     2821 tcggagcgtc aagcctcaaa ctatgattgg ttatcaggtg attgagaata cccctgctat
     2881 caagttcaag atcgcctatc tggaaaaggc tttattggac tatttatatt taaattctga
     2941 gatccagtct ttagccgatt ttgaggaact tcgctggaac cggacacaat tacgtgggct
     3001 gcttgatcat tcgattttcg ccaagtatgt caaactattc gataaaagag ccctgaaaaa
     3061 cagagtcaat caattcctgg agtatctgaa tgcttgattt acagcaaata aaaaatgaat
     3121 attcagaacc gctgcagggg tatgaacggg caatccttcg tgaatacctg cagtataaga
     3181 tcttacaggc gatttttgaa agcaaacaag ccagcatgct atcgtttctg ggtggcacag
     3241 ctttacgcat cgtacatgga aatagccgtt tctcagaaga tatcgatctt gacaattttg
     3301 gtttgagctc gcaggcgttt ggtgaattga tccagaaggt gaagctgttt ttagagctgg
     3361 aaggattcct ggtggagacc agcatggttg cgaagggcgc attccactgc gatctgcggt
     3421 ttccagaact gctttacaag caaggtttgt cacctcttca tcaggagaag atactcattc
     3481 aactggacac gatagctcaa gggtacacct atgcgccgga ggtcaagata ctcaataaat
     3541 ttgatgtctt tactgagatc agagtaacgc cgttaaatgt tttgctgtca caaaagattt
     3601 atactgcgat taatcgcaaa cgcccgaaag gaagagattt ttacgatatc acatatctat
     3661 tcagtaaaac caggccggat tttggattta taaaacagaa gatgggcatt acctctcccc
     3721 aaagtctacg cgaggaactg cttctgaaga tcggggatat tgattttgat gccctggcgg
     3781 gcgatgttgc cccattttta atcacgaagg agcaggtcat gagagtggtt aaattcagag
     3841 aattctggaa gcaggtggat ttagattaat cagggcggtg taagactcgc tgataggttg
     3901 acgcggaggc acggagatgg agagaccatc tcagtgtctc ttaagcaatg aacctggtgc
     3961 tagttcggga atttttaccc accgtgggca ggggacccac cctctccgca ggtaatatcc
     4021 gccaatccac acgccaattt tagcaagaca tcgtggagat tggcggatat ttttccatta
     4081 accctgcgcc aatgttttcc ttttattgtc ttcagttcta tttaatactc cgtcgtttac
     4141 cagataggct ggcgattttg ccctcggcag cctagggacc gagcagccac gcgcgtgcgc
     4201 cgtttggcgt gaggacaatt caggaagcca tagcttccgc cgtatgtggc gggcatggga
     4261 catacccgtc catttcaata cccgcaaggc tcatatttgt atcatccggt tgaatcatac
     4321 cgttgaggtt acttcatcca gatgagtgcg aagtctgctt acggcaattg tgatgtttat
     4381 cacttgcttc ggcaatgatc ttgatctaaa atcttgttaa aggttacctt tattcctacg
     4441 tcatgaaaca acgggagcag atagttgttc tgtctctatc aaaagtcttc aggagtttcg
     4501 ttttgaccat tagtttgacc tgaggctgcc gtttgcaaaa ggctgacaca ctcatccgca
     4561 caaagctgcg cctgcccttc accagaccag agctggtccc gcggctgcat ctccaggagc
     4621 aaattgcgca ggggttgcgc ggaccgctta ccctggttac cgcccccgct ggttttggaa
     4681 agaccaccct gattgcatcc tatattacaa attgcggatt gcccatcgcc tggctgtctc
     4741 tagataagga tgataaccaa gcagggcgct tcctgaacta tctggtcgct gccttacaag
     4801 aggctgacta tacaatcggg aacgaggccg ctcaactggt ggcggcggca cagcaggcac
     4861 cgcctgaagc gatcctgacc agtctcatca atgacctgga taccagcgaa ggggagatag
     4921 tcctggtatt agatgactac cagtttatta aaaaccaggt tgtacatgat gttgtaactt
     4981 ttttactcga gcattgccca tatacatttc atctgttgat cgctacccgt tcggacccgc
     5041 cgctgccaat aacgcgtttg cgagcgcgcg gccaaacgat tgaacttcgc accgctgatc
     5101 ttagctttac cgaggtggag gcagctgtgt tcctgaacga tgtcatgggt ctcaacctgg
     5161 atgcaggatc ggtcgcggcg ctcgcagaac gcaccgaggg ctgggttgcc ggattacaga
     5221 tggcagctct ctcgatgcgc gatcgggaag atgtacgcgg gtttatcgaa gggttctcgg
     5281 ggactaaccg ctacattttg gactatttga tggaagaagt cctcgccagc cagccgccag
     5341 aaatccagcg ttttcttctg tacacgtcga ttttggagcg cctaaccgcc ccgctatgcg
     5401 atgcggtaac cgagattcaa gaaaagatgg atagtagtaa cgaatctata ccctcttctc
     5461 gcgattctca atctatcctg gagtacctgg atcgggcgaa tctctttctt atccctctgg
     5521 atgatgcgcg tgtttggtac cgctatcatc acctcttcgc cgatctgttg cgcgcccggc
     5581 tgcaccagac ccacccagat ttgatccatc acttgcatat gcgggcctcc gcctggttgg
     5641 aagaaaaagg cattatttcc gaagcgattc accatctctt cgctatacag gagattggac
     5701 gggcagcaga cctgatcgag cgctacggac cagtgtatct ggcacaaagc gacccttcga
     5761 ttttccagat ggccgacagt ctcccagcgg aaatgcttat cgcccgacct aaaattggga
     5821 tttacctggc ctggtttctc atcgtccagg gtcacaccag aaaagccgtc caaatactgc
     5881 acgacgtgag gcgacaactt gcagacctcg attctggtca acaatggatg cagacgttta
     5941 tcgccacggc tctcgctttt cttgccccac cgggtagcaa tcctgatgtt tatcccctcc
     6001 ctgatttcag gctattggat gagatccctg ctgacgagat ggtcttgcgt aacgcggcag
     6061 atttcctcta cgggatggca ctggcaagac gaggggaatt acaccgcgct gtggaggtct
     6121 cgctcgagtg tatccagagg gagaggttac aacgtggaac actaaccatc ccaacattag
     6181 ctcccttcct gtcccgtttc tacctgatgc tgggtcgttt acacgcatcc gccaacctgt
     6241 gccgcgaatt tttagacccc atccaggagc gaggcatccg cttcgtctat actgccggca
     6301 gcatgaagat cgacctgggg gaggtgctgt acgaatggaa ttgtctggat gaggccgagc
     6361 tgcatatccg agacggcctt cgagacaatg aaccctggcg gaatattatg acggatggct
     6421 tcggactggt agccctgacg cgcctccttc agtcaaaggg gaaatacgcc gaagcgatgc
     6481 aggtcgtaga gaagtttgag aagagactgc gagaggactc acagccgcgt gaattcgatg
     6541 aggatttacg caccctgaga gttcgcgtgg agcttttcag cggggatctc caaaaccctt
     6601 ccctttgggc cgatcagatt ttgctgagcg aggatttcga actccacgag gagtattacc
     6661 ggctcaccct tgcccgcatc cgcctggcgc agggcaggta cgccgaagtc gaaaaaatcc
     6721 ttgccgggat gacaccccag attaccgctg gcagccggat aaccaggcaa ctggaatgca
     6781 atctgcttct ggcccttgca gtggccgggc agcagcgcct accagaagct tttaaattca
     6841 tcgaatccac ccttgccctt gctgagcctg aaggatatat gcggatcttt ttagatgttg
     6901 gagagccagt tcgagaactg ctgactgcct atctgcggtc agatgctcct gctcataaaa
     6961 tttacgctcg gaacatactg gatgcgtttt ccctcacgag acaaacaagt tctccagggc
     7021 ctcagccagc cgggctgata gaaccccttt caggacgcga gctggaagtc ctcgagttat
     7081 tggctctggg aaagtccaac cgagagatcg ccgggcagct cttcgtttcc ccaggtactg
     7141 taaaagctca tacatccagt atctatcgca agctggacgt cgccaaccgg accgaggcgg
     7201 tggcgcgtgc caggcagctc gatatcctcc cctaaccccc aaatttagcc tcgcagacac
     7261 acaagtttac aactaaagcc tggaaataaa ccccaaatat cccttttggc agatgcgggc
     7321 tgcctcgttg cgcgctaaat tattgtcata tacggataag gagagctgac atgccagaat
     7381 a
//
LOCUS       DBNGSX010000397         6616 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_867281, whole genome shotgun sequence.
ACCESSION   DBNGSX010000397 DBNGSX010000000
VERSION     DBNGSX010000397.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6616)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6616)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6616
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_867281"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="AC16CB_11855"
     CDS             complement(<1..394)
                     /locus_tag="AC16CB_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR3343209.1"
                     /translation="MFKKLMMLAVVFMLLVSACAPAATPTEAPAQPTEVPAQPTEAPA
                     EPTQPPAPSGGVVKATPGQALKMVFLPKNLGNAVFDEAHEGALEAAAELQNPEELQYL
                     APASGAAGTEQIPIITNATTQGVNAIMIS"
     gene            complement(466..1485)
                     /locus_tag="AC16CB_11860"
     CDS             complement(466..1485)
                     /locus_tag="AC16CB_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3343210.1"
                     /translation="MMRRIERLKSWEGLLLAILAVIVVLNTFTTPHYLQVQNQINLFQ
                     LHIEKIIVALIMTFIIINAEIDLTVGSVMGLAGCVLAYLFERGVPFEMCILAGLLTGL
                     LAGAINGFMVSYVGLTSLIVTLAGLIGYRGLAHVLLENRSVGNFPAWFDRLGQQPLIG
                     PFPLALIIFFVLFIIALIILQWSGAGRYVFVIGNNREVARYSGVNVRRIKMILFIVSG
                     FFAAFAGLLLAARLGSMRGDTALGTELEVITMVVLGGVSIFGGTGSLFGVILSILIIL
                     NLRNGMGLLNITGEIQTGVIGVLLILSVLGPNVGQGLRDAFNRRRRVQHVAVGDENLG
                     KEDNP"
     gene            complement(1475..2542)
                     /locus_tag="AC16CB_11865"
     CDS             complement(1475..2542)
                     /locus_tag="AC16CB_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3343211.1"
                     /translation="MERLKRHFRPEQLRELSLVFLIVLIVLFFGTQIEGYFSPRIFNR
                     VTSDVAIIAVVAIGETLVLLTRNFDLSVGSIVGLTAFAVGKLLTFNPDMLPIVALLSA
                     IGLGAAMGLLNGVFVSFGRVPSLIVTLGTMAIYRSTLVAYPAGTKNVVTQDLPQWLLD
                     LGGMKLFSIGDFDIRPVVAGGLVVFVVFQLVISYLRFGRRLYAIGSNPEAARIGGLPS
                     QQIVLFAFILCGAMAGLGGFMFLVRYGNLTPAAAQGMELQVIAAAVVGGVSTNGGSGT
                     MIGAVLGALMINLLQQSLLRWLVISNFWVGALLGVLILVSVTIDAVIINRLRDIWTRR
                     SPGINPADEKVVIEGEESYDA"
     gene            complement(2542..4068)
                     /locus_tag="AC16CB_11870"
     CDS             complement(2542..4068)
                     /locus_tag="AC16CB_11870"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR3343212.1"
                     /translation="MMTSVEAKAPILQIRHISKRFDTTQALDDVSLDLYPGEIHALMG
                     ENGAGKSTLIKIMTGIYHSDKGEMLLDGKPIRVSNSIDAQAYGIAAIYQEPMVYPDLN
                     VAENIFISHRSQGVIVNWRKMYQDAEDILAKLDVKLDVRMPARGLTLAAQQTIEIAKA
                     ISLNVRLLIMDEPTASLSAHEVEQLFKLTRSLRNQGVSILFISHRMEEVFEIADRVTI
                     FRDGRWISTLPRSEITRERVIRDLVGRKIEDFFAKSGVQRGELLMSVQGLHKENVFSG
                     INFNLYRGEVLGFAGLVGSRRTDVGLALFGVEPADSGEIILDGKVEKIHSPEHALRTG
                     IAYVTEDRRQIGLTMPMSITSNISLPMLRQYLSRFGLVKRRGEEATAEHYRERLSIRT
                     PSIRQPVAMLSGGNQQKVMLSKWLNTRPQILILDEPTRGIDVGAKAEVHQIINELAAQ
                     GLGIILISSDMPEIMAMSDRILVMREGRQMGIFTREEATQEIILTAAMGQSDSAAEVV
                     "
     gene            complement(4114..4899)
                     /locus_tag="AC16CB_11875"
     CDS             complement(4114..4899)
                     /locus_tag="AC16CB_11875"
                     /inference="COORDINATES: protein motif:HMM:NF012669.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR3343213.1"
                     /translation="MTTYERRHSLLEILRKHPGLRVSEMAKALGVSEGTVRNDLNALK
                     EEGRLKRVHGGAVLIDQDQFQNDSFVRRYNQNVAAKLAIAREAALLVMDGDSILLDAS
                     STSYYLAHAISARNRLRVMTNGFEVARELAQNTSNTVILIGGVVNNDSSSVTGLLSEQ
                     IIAEMHIEKAFFSCSGFSVERGMTEVHFEEAQLKRKAIESTKQVIALIDSSKFGKEDL
                     TPFARPMQITHLFTDAGITSEWKTRLQEANILFTVCGEDSIAI"
     gene            5267..5416
                     /locus_tag="AC16CB_11880"
     CDS             5267..5416
                     /locus_tag="AC16CB_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343214.1"
                     /translation="MPKLLVEYAETAARYELECLRLGSPTQGLTDEQLLQLCKQFGID
                     PNRFV"
     gene            complement(5423..5797)
                     /locus_tag="AC16CB_11885"
     CDS             complement(5423..5797)
                     /locus_tag="AC16CB_11885"
                     /inference="COORDINATES: protein motif:HMM:NF024637.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR3343215.1"
                     /translation="MKSTAAWVVAIVLILVVLLALPGVLRFAGLSGPWYGGMMGGRGM
                     MGGYYGFSPLGFLGMGFMLLVPVALLVLVVAGIVAVVNSLNRGNSPASYPTAGNSRAC
                     PNCAKPAQPDWNTCPYCGTSLN"
     gene            complement(5932..>6616)
                     /locus_tag="AC16CB_11890"
     CDS             complement(5932..>6616)
                     /locus_tag="AC16CB_11890"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR3343216.1"
                     /translation="AVFTQSLAGFGSALVAMAFLPELLGIRVATPLVAMVAMTLEGLL
                     LVRYRAVLNLRAVWQVSVASLVGIPLGMLTLKRIDEGAAMTILGVLIFAYALYNLFEL
                     RLPEMNHPAWAYTFGLLAGMLGGAYNTSGPPVVIYGNSRRWLPVEFKSNLQGFFFFNS
                     IVVVFGHALSQNITPLVWQDYLMAIPALALGILAGVSLDRMINPVVFRRLVLGLLLVM
                     GGRLILQGF"
BASE COUNT         1739 a         1824 c         1586 g         1467 t
ORIGIN      
        1 tggagatcat gatggcattg acgccctgcg tggtggcgtt ggtgatgatg gggatctgct
       61 cggtgccagc agctcccgag gcaggagcga ggtattgcag ctcctccggg ttctgcagct
      121 ctgcggcagc ttccagcgcg ccttcatgtg cctcatcgaa cacggcgttg cccaggttct
      181 tgggcaagaa gaccatcttc aatgcctgcc ctggggtggc tttgaccacg ccacctgagg
      241 gggcaggggg ttgtgttggc tcggctggcg cctcggttgg ctgtgcaggc acctcggtcg
      301 gttgggctgg cgcctcggtc ggtgtcgccg ctggcgcaca cgctgagact agcaacataa
      361 atacaactgc aagcatcatt aatttcttga acatcttctt tctccttatg gtgagattaa
      421 acgaggatcg aacggtctct tgaggctttg ataatatgct atcgtttatg ggttgtcctc
      481 ctttccaaga ttctcgtctc caacagctac gtgttgaaca cggcggcgac ggttgaaggc
      541 atccctgagt ccttgcccca cattgggccc gagtaccgac aggatgagca gaacaccgat
      601 tacgccggtc tgaatttcac cggtgatatt gagcaatccc atcccgtttc gcagattcag
      661 aatgatcagg atcgacagga taacgccaaa cagcgagcct gtgccaccaa aaatgctgac
      721 cccgcccagt acgaccatcg tgatgacttc gagttcggtg cctagtgccg tatcgccacg
      781 catcgagcct aatcgtgcgg ctaataacaa tcctgcaaag gcggcaaaaa agcccgacac
      841 aatgaaaagg atcatcttga tccggcgcac gttgacaccc gaatagcgcg ccacttcgcg
      901 gttatttccg ataacgaaaa cgtagcgacc agcgccggac cattgcaaga ttatcagggc
      961 gatgatgaac aagacaaaaa agatgatcag agccaatggg aagggaccga tgaggggttg
     1021 ctgtcctagg cggtcaaacc aggcgggaaa gttgcccacc gagcgattct ccaagagcac
     1081 atgtgccaga ccgcgatagc cgatcaaccc ggccagggta acgatcaacg aagtcaatcc
     1141 cacgtaggag accatgaaac cgttgattgc tcctgccagc agtccagtta atagtccggc
     1201 taaaatgcac atctcgaaag gcacacccct ttcaaacagg tatgctagca cgcagcccgc
     1261 caaccccatc accgagccga ccgtcaggtc aatctcggcg ttgatgatga taaaggtcat
     1321 gatcagcgca acgattatct tctcgatatg cagttgaaac agattgatct ggttctgtac
     1381 ctgcagatag tgaggtgtag taaaggtatt taagacaacg atcacagcga ggatagcaag
     1441 caacaagcct tcccaactct tgagccgttc gattctacgc atcatagctt tcctcccctt
     1501 cgatcaccac cttttcatca gctggattga tcccagggct tcgtcgagtc caaatatcgc
     1561 gcagccggtt gatgatcacc gcgtcgatgg tgacagatac caagatcaac acgcccaata
     1621 aagcgcccac ccagaaattg ctgatcacca gccagcgtag caggctttgt tgcagcaggt
     1681 taatcatcag ggcgcccaac accgcaccga tcatcgtacc gctaccgccg ttggtgctaa
     1741 ccccaccgac caccgccgcg gcgataacct gcaattccat cccctgcgcg gctgcgggtg
     1801 tgagatttcc atagcggact aaaaacataa aacctcccag tccagccatc gctccgcaaa
     1861 gaatgaaagc gaacaggaca atctgttgtg aaggcagacc accgatccgc gctgcttccg
     1921 ggttcgagcc aattgcgtag agacgacgac caaatcgcag gtaggagatc acaagttgaa
     1981 aaacgacgaa taccaccaat ccaccggcta cgaccggtct gatgtcaaaa tcaccaatgg
     2041 aaaacagttt catgccaccg agatcgagaa gccactgtgg caaatcttgg gttactacgt
     2101 ttttcgtgcc agccggatac gctaccaggg ttgagcggta aatcgccatg gtcccaaggg
     2161 taacgatgag ggaaggcacc cggccaaacg agacaaacac accgtttaac aggcccatcg
     2221 ctgcaccaag gccaatggct gataaaaggg ctactatcgg cagcatgtcg gggttgaagg
     2281 tgagcagctt gccaacggca aacgccgtca agccaacaat tgagccaact gaaagatcga
     2341 agttgcgcgt cagcaacacc agggtctctc cgatcgcgac cacggcgata attgccacat
     2401 cgctagtgac gcggttaaag atgcgtgggc tgaaataacc ctcgatctga gtgccaaaaa
     2461 ataggacgat gagcacaatc agaaagacta ggctcagctc acgaagctgc tctggacgaa
     2521 agtgccgttt caatcgttcc attatacgac ctccgctgcg ctatcacttt gtcccatagc
     2581 cgcagttaag atgatttcct gtgttgcttc ttcgcgagta aaaatgccca tctgtctccc
     2641 ctcgcgcatc accaagatgc gatcgctcat tgccatgatc tcaggcatgt cggatgaaat
     2701 caggatgatg cccaagcctt gggcagcaag ctcattgatg atttgatgca cttcggcttt
     2761 tgcgccaacg tcaatgcctc gtgtgggctc gtcgaggatg agtatttgcg gacgtgtatt
     2821 cagccacttg ctcagcatca ctttttgttg gttgcctcca gaaagcatag cgactggttg
     2881 ccgaattgaa ggtgtgcgaa ttgagagacg ttctcgataa tgttcagcgg tagcttcctc
     2941 accacgcctc ttaacgaggc cgaatcgcga caggtactgg cgtagcatcg gcaaagatat
     3001 gttgctggtg atggacatgg gcatcgtcag accgatctgg cgccggtctt cggtcacgta
     3061 tgcgatccca gtccttagtg catgctccgg ggaatggatt ttttccacct tgccgtccag
     3121 gatgatttca cccgaatcag cgggttctac gccaaacaac gccagaccaa cgtcggtgcg
     3181 gcgtgatccc accagaccgg caaaacccaa aacctccccg cgatacaaat taaaattaat
     3241 tccggaaaat acgttttctt tgtgcagacc ttgtactgac atcaacagct caccgcgctg
     3301 caccccgctt tttgcaaaaa aatcttcgat cttgcgccca accagatctc ggatgactct
     3361 ttctcgtgtt atctcgctgc gtggcagcgt tgaaatccac ctgccatcac gaaagatggt
     3421 cacgcgatcc gcaatttcga atacttcctc catacggtga ctgataaaga gaattgacac
     3481 gccctgattg cgcagcgagc gcgtcagttt aaataactgt tccacctcat gggctgagag
     3541 ggaggcggtc ggttcatcca ttatcaggag gcgcacgttg agtgagatgg ctttcgcaat
     3601 ttcgatagtc tgctgggcgg caagtgtcaa tccgcgcgct ggcatgcgca cgtctagctt
     3661 aacatctaac tttgcaagaa tatcttccgc atcctggtac atcttacgcc agtttacgat
     3721 caccccctga gaacggtgcg aaataaaaat attctcggcg acgttcaaat ccggatatac
     3781 catcggctcc tggtagattg cagcaatgcc gtaggcctgg gcatctatcg agttactcac
     3841 ccggatgggt ttcccatcta acaacatttc ccctttatcc gaatgatata tccccgtcat
     3901 gatcttaatc agcgttgatt taccagcgcc gttctccccc atcaaggcgt gaatctcacc
     3961 cggataaagg tcaagagaca catcatccag tgcctgggtt gtatcgaagc gcttggaaat
     4021 atgccttatc tgcagaatag gagcttttgc ttcaacactc gtcatcattg atcaatgcct
     4081 ttttcaattg gtggctcaat ggcctctcac cgctcagatc gcaatcgaat cttctccgca
     4141 gactgtaaat aagatgttag cctcttgcag gcgggttttc cattcgctgg taattccggc
     4201 atctgtaaac aagtgcgtaa tttgcatcgg acgagcgaaa ggggtcaggt cttctttgcc
     4261 gaatttcgaa gagtcaatca acgcgataac ctgttttgta gattcgattg ctttacgttt
     4321 cagttgcgcc tcctcaaagt gcacttccgt catcccgcgc tcgacgctaa aaccactgca
     4381 agaaaaaaac gctttttcga tgtgcatttc tgcaatgatc tgctcgctca acagacccgt
     4441 tacggaagag gagtcgttat tgactacccc cccaattaga atgacggtgt tcgaggtgtt
     4501 ttgagccaac tcccgcgcaa cttcaaatcc attggtcatc acgcgcaatc tgtttcttgc
     4561 ggatattgcg tgcgccaagt aataggatgt actgctcgca tccagcagaa tcgagtcacc
     4621 atccatgact aaaagagctg cctcacgcgc aatagcgagc ttggcagcta cattttgatt
     4681 gtaacgacgt acaaaagaat cattttgaaa ttggtcttgg tcaattaaga cagctcctcc
     4741 gtgtacgcgc tttaggcgtc cctcctcttt tagagcattg aggtcgtttc gaacggtacc
     4801 ttcggataca cctaacgcct tagccatctc cgagacacgt aaaccgggat gtttacgcag
     4861 aatttctaat agagagtgtc ggcgctcgta agtggtcaaa tcagacaatc cttagaatat
     4921 tttgttgttt tttgttgatt tcgataaaac tatcatacaa tttttgtcat tatttgtcaa
     4981 tatttaaata atatttcagt gtatgtccca aaattggtgg ggaaaatcaa agagaacagg
     5041 aagaccggcg cgaccctaaa atgaacatgg ggggtgccac aattgaacga ctgacccacg
     5101 acactaatta ccacagcgac atccaccctg acagccagat gtataaaata aatcccttta
     5161 acgactgtcc cttatgatca tttattggat atcacaaaat aacctcagct taggtataaa
     5221 gtccaggtta ccggcttctg ctgcatttcc gacatcggaa atgcgtttgc ctaaattact
     5281 ggtggaatac gccgagacag ccgcgcgcta cgagctggag tgcctgcgcc tgggctcacc
     5341 cactcagggt ctcacagacg aacagttgct ccagctttgc aagcaatttg gcattgaccc
     5401 gaaccgtttt gtctgaaaaa gactaattca aggatgtccc gcaatagggg caggtgttcc
     5461 agtcaggctg ggcgggcttg gcacaattcg ggcaagcgcg gctattccca gcagttggat
     5521 acgaggccgg agagttgccg cggttcaggc tattcaccac ggctacaata cccgccacca
     5581 ccaacaccag caaagccacc ggcaccagta gcataaaacc catccccaaa aaacccaagg
     5641 gggaaaagcc gtaataacct cccatcatgc cgcgcccacc catcataccg ccgtaccatg
     5701 gtccagacag gccagcgaag cgcagcacgc cgggtaaggc tagcaaaacg accaagatca
     5761 atacaatggc aaccacccat gcagctgtag atttcacgtc aaacctcctt caagcattga
     5821 gaaaaacaac ctttagattt cagcacatct tcaggatacc aactcaatgt gaaaaagttg
     5881 tgaagaattc gtgaatacct ggcaaagaga tcaacacaaa cctggttcat ctcaaaaacc
     5941 ctgtaaaatc aagcgcccac ccatcacaag cagcaaaccc agcaccagcc tgcggaaaac
     6001 gactggattg atcatcctat ccaggctgac cccagccaag atacccagcg ccagggcggg
     6061 gatcgccatc aagtagtcct gccaaaccaa cggggtgata ttctggctca aggcatgccc
     6121 aaagaccacc acgatgctgt taaaaaagaa gaatccttga aggttactct taaactccac
     6181 cggcagccag cgacgggagt taccatatat gaccaccggc gggccagacg tgttgtacgc
     6241 accacccagc atacctgcca acaacccaaa tgtatacgcc caggcaggat ggttcatctc
     6301 cgggagacga agctcaaaga gattgtaaag agcataggcg aagatcagca cacccaaaat
     6361 ggtcatagcg gcgccctcat cgatgcgttt gagggtcagc atgcccagcg ggatgcccac
     6421 cagagatgca acagacacct gccacaccgc tcgcagattc agcactgcgc ggtagcgcac
     6481 caacaagaga ccctccaaag tcatggctac catcgctacc agaggagtag cgacgcgtat
     6541 gcccaataat tctggcaaaa aagccatagc caccaacgcc gagccaaatc ccgccaggct
     6601 ctgtgtgaat acagcc
//
LOCUS       DBNGSX010000398         4979 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1350872, whole genome shotgun sequence.
ACCESSION   DBNGSX010000398 DBNGSX010000000
VERSION     DBNGSX010000398.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4979)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1350872"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="AC16CB_11895"
     CDS             <1..656
                     /locus_tag="AC16CB_11895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3343217.1"
                     /translation="AHGMPDKRIVTFPWGIDLQHFSPGQPPASSDQPSTFVILSTRGW
                     EPIYGVDVIARAFAIAAAQRPELRLIMLGNGSQAAQLRQIFLGARVEDHVLFPGQVGH
                     DDLPRYYRMADLYLSASHSDGTSISLLEAMACGRPALVSDIPGNREWVQPGINGWLFP
                     DGDEKALAESILNAVENRQQLPEMGRRARQIAEQRADWEKNFPILLRAHEIALSDPK"
     gene            653..2104
                     /locus_tag="AC16CB_11900"
     CDS             653..2104
                     /locus_tag="AC16CB_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343218.1"
                     /translation="MKLLAALKSPFLQMGKIDKPLLALFLAINVLVLINSILHNPEIG
                     YDGKSHLIYMRVLPYRLPTVEETREFFSPPLPYFLPSLLDKACLNLKEIRDVSVKIDD
                     CLRSTGKFAQFINFILSLGVTFLFTKIAGVLRPDNRFWKFSSLALLGVMTVYYKTFAQ
                     VRGEPYVVFLTVLAIYYLSKKVHDRGQVTLTSGIGLGIILGLLSLSRQWGFMLMPAIF
                     GLMGLVWIFDQKHRWQFVTSLSISFVVAFLVCGWFYLHLYFEHGSLLTFNRSPQAFSF
                     SNQPPSFYRQSGLSNLMLFKSPTRPTFENQFFPILYSDVWGDYWGYFVYIQDRSSLSF
                     NRSSLGVMEKKGNREIINPFLGRANAVSIFPSLIFLAGILTSAASNHKLLRRDQEEKN
                     RSLFYMFLLMFVVVSFMLYLYFLISYTMTDQDSTIKATYMLHVLIIFPLLGAEFLERV
                     RQRKQIFYYGCMVALGLVLVHNLPAMITRYRMF"
     gene            2127..3545
                     /locus_tag="AC16CB_11905"
     CDS             2127..3545
                     /locus_tag="AC16CB_11905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR3343219.1"
                     /translation="MKTIFLIKGEIDRIFLILFLVINLLVLVNNVLHNPVVGYDGTEH
                     LKYIRVLPYRLPDTADTREFFSAPLPYVFPSLIDKACLRSKPVNSGSIAIDNCLIFAG
                     KFAQLINFVLSLGVTFCVGSIARIMQPESRWGKISALALLGVLTVYYKTFTQVRGEPY
                     LILFTAWTLYLVARMIQAPEQVTWRSGLKLGVVIGLALLSRQWGFMLLPALIGLFALV
                     WLLDSHWGREWANAMTVAALVAFLICGWFYLSLYIRYGTFMAFNRTPLVFSFSNQPST
                     FYRNTGLKNLLLFKSPIRGTFNNQLFPILYSDTWGDYWGHFVFIQDRSYLGEMGYGNI
                     QQITPYLGRVNAVSMYPSLIFLVGMLTGVFYSTKLVKGDAGERRRALLNIFLLFYLIV
                     SFSLYVVFLNMYPLLDQGDTIKATYMLHVLVVLPILGAEFLEGLRARSPKAYLVSLAL
                     LGLVFAHNLPAMITRYKIFSIP"
     gene            3548..4336
                     /locus_tag="AC16CB_11910"
     CDS             3548..4336
                     /locus_tag="AC16CB_11910"
                     /inference="COORDINATES: protein motif:HMM:NF014409.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylyltransferase domain-containing protein"
                     /protein_id="HIR3343220.1"
                     /translation="MPEVSIAIIQARMASSRLPGKVLLDIGGQPMLIRVIERVKRART
                     VDRTVVATTTDPSDDPVERLCQERGYPCYRGSAQDVLDRYYQAARSHEAEIVVRITAD
                     CPVIDPGLIDETVKALFEPSPVSSQEPAIAYDFAANRLPPPWGRTYPIGLDVEACTFA
                     GLETAWVEARQPHQREHVMPFFYENPQRFRICLLNHHTDLSAYRWTVDSAEDLELLRQ
                     IYSRFGGRDDFSWLEVLALFEREPELLQINAVVMHKDYREIDER"
     gene            4326..4979
                     /locus_tag="AC16CB_11915"
     CDS             4326..4979
                     /locus_tag="AC16CB_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343221.1"
                     /translation="MSDKSTLSIKSVFDKGLVISIATWLLLSLVFGLAYTQSHLYNDN
                     QNTKFLHGLAMGGMGLLKEDWLANTADPLPAFSMLVSFTFAFLAERLFYVYFALLMGV
                     YAYSILGIASSLYGMNRSPAKYLAYFVVILAIHSIQGQIFARKVLDFDVEILHFGLAG
                     QYLLGLDFQNSSFGVFLLLSMQAFVQRKTVWAILWLSLASRFHSAYLFSAALMTLAY"
BASE COUNT         1065 a         1310 c         1231 g         1373 t
ORIGIN      
        1 tagctcacgg catgccggat aagcgcatcg tcactttccc gtggggtatc gacttgcagc
       61 atttctcgcc tggtcagcca ccagcatcaa gtgatcagcc atctactttc gtaattctct
      121 ccacgcgcgg ctgggagccc atctacggtg tagatgttat cgctcgcgcc ttcgccattg
      181 ccgctgcgca gcgccccgaa ctgcgcctga tcatgctggg caatggatcg caagccgctc
      241 aactgcgcca gattttcctg ggtgccagag ttgaagatca cgtcctattc ccaggtcagg
      301 taggccatga cgacctgcca cgctactacc gcatggcaga cctgtacctg agcgcttcgc
      361 acagcgatgg tacctccatc tccttgctgg aagccatggc ttgcgggcgt cccgccctgg
      421 tctccgatat ccccggcaac cgcgaatggg tgcagccggg catcaacggc tggctgttcc
      481 ccgacgggga cgagaaggcc ttagcggagt ccatcctgaa tgcggttgag aaccgccagc
      541 aattgcccga gatgggtcgc cgcgcccgcc agatcgccga gcagcgcgct gattgggaga
      601 aaaacttccc aattcttctc cgggctcatg aaattgcctt gagtgatcca aaatgaagct
      661 ccttgctgct ttaaaatcgc cttttctcca aatgggaaag atcgataaac ctctgcttgc
      721 tctattcctg gcaattaatg tactggtctt aatcaacagc atattgcaca atccagaaat
      781 cggctatgat ggaaaaagcc atttgattta tatgcgggtt ttgccatatc gattgcctac
      841 ggtcgaagag acgcgcgaat tcttctcccc tcctttgccc tattttcttc cttctcttct
      901 cgataaagcc tgcctgaatc tgaaagaaat tagagatgta tccgtaaaga ttgatgattg
      961 cctaaggagc acaggcaagt tcgctcaatt cattaacttt atcttatcct taggtgtcac
     1021 ttttcttttc accaaaatcg ctggagtgct gaggccagac aatcgattct ggaaattttc
     1081 atcactggct ttgttggggg ttatgacggt ctattacaaa acgtttgccc aggtacgcgg
     1141 tgaaccgtat gtggtttttc tcactgtttt ggcgatatat tatttatcga agaaggttca
     1201 cgatcgtggg caagtgacgt tgacgagcgg tatcgggctg ggaatcattc taggccttct
     1261 gagcttgagt cgccagtggg gctttatgct gatgccagcg atctttggtc taatggggct
     1321 ggtttggata tttgatcaaa aacatagatg gcaattcgtc acgagccttt cgatcagttt
     1381 tgtcgttgca ttcttggttt gcggctggtt ttatctacat ctttattttg agcatggctc
     1441 cttgctgact ttcaatcgct ccccacaagc gttttccttc tcaaatcagc cgccttcttt
     1501 ctaccgccaa tccggattaa gcaacctgat gctgttcaag tctccgacca gaccaacctt
     1561 tgaaaatcag ttttttccaa ttctttattc agatgtttgg ggggactact ggggttattt
     1621 cgtatatatt caggatcgat cctccctgag tttcaacaga tcttctctgg gagttatgga
     1681 aaaaaagggg aaccgcgaga ttatcaatcc ctttctaggg cgggcaaacg cggtttccat
     1741 atttccgagc ctgatctttt tagcagggat tttaactagt gctgcttcta accataaatt
     1801 gcttaggcgc gatcaagaag agaaaaatcg atccctattc tatatgttcc tattgatgtt
     1861 cgtcgttgta tcattcatgc tttacctgta ttttttaatc agttatacga tgacggatca
     1921 ggatagcact atcaaagcca cttatatgct gcacgtgttg atcatatttc cgctcctggg
     1981 cgcggagttc ctggaaagag taaggcagcg caaacaaata ttttattatg gctgtatggt
     2041 tgctcttggg ctagtgttgg ttcacaatct gcccgccatg atcactcgat accggatgtt
     2101 ttgatgaatg aagtctgatt actgctatga aaacaatctt tcttatcaaa ggtgagattg
     2161 atagaatatt tcttatcctc ttcctggtaa ttaacctgtt ggtcttggtt aacaacgtgc
     2221 tccacaaccc agtggtcgga tacgatggca cggaacacct aaagtatatc cgtgtgctgc
     2281 catatcgatt accggatact gccgatacac gcgagttctt ctcggctccg ttgccttatg
     2341 tgttcccctc gctgattgat aaagcctgcc tccgctcaaa accagtcaac agcggatcaa
     2401 ttgctattga caactgtctg atattcgctg gcaagtttgc acaactcatc aatttcgttc
     2461 tttcattggg ggtcaccttt tgtgtcggga gtatcgcccg gatcatgcag cctgagagcc
     2521 gttggggaaa aatctcagca ctggctttgc tgggggtatt gacggtatat tacaagacct
     2581 tcacccaggt gcgtggggag ccttacctaa ttttattcac ggcatggacg ctctacttag
     2641 ttgctaggat gattcaagct cccgagcaag tgacgtggag gagcggcctg aagctcgggg
     2701 ttgtcatcgg gctggcattg ctcagtcgcc agtggggttt tatgctcctc ccagcgctca
     2761 tcggactatt cgccctggtt tggctcttgg atagccattg ggggcgggag tgggccaatg
     2821 ccatgaccgt cgccgctttg gtcgctttcc taatctgtgg ttggttttat ctgtctctct
     2881 acatacgcta tggcacattt atggctttca accgcactcc cctggtattc tccttctcca
     2941 atcagccaag cactttctac aggaatactg ggctaaaaaa cctcttgctg ttcaaatccc
     3001 cgatccgagg cacttttaac aaccaactct tcccgatttt atattcggat acctggggag
     3061 attactgggg acattttgtg tttatccagg atcgctctta cctaggagag atggggtacg
     3121 gaaatatcca gcagattaca ccctatctgg gccgggtcaa cgcagtctca atgtatccca
     3181 gtctaatctt tttggtcggc atgctgaccg gcgtatttta ttccacaaag ctggtcaagg
     3241 gcgatgctgg cgagagaaga cgggcactct tgaatatatt cctgttgttt tacttgatcg
     3301 tgtctttctc tctgtatgtt gttttcttga acatgtatcc cctgctggat caaggcgata
     3361 ccatcaaggc cacttatatg ctacacgtgc tggtggttct gccgattctg ggggcagagt
     3421 tcctggaagg cctgcgagcg cgctccccaa aggcatatct tgtcagcctg gcactgcttg
     3481 ggctagtttt cgcccataat ttgcctgcca tgatcactcg atacaagata ttctctattc
     3541 cctaagtatg cccgaagtat ccatcgccat cattcaagcc cgtatggctt cttcccgctt
     3601 gccgggcaaa gtgctgctcg atatcggcgg gcagcccatg ctaatccgtg tcatcgaaag
     3661 ggtgaaacgg gcgcgcaccg tggatcgaac tgtcgtcgcc accacgaccg atccgtcgga
     3721 tgacccggtg gagcgcctgt gccaggaacg cggttacccc tgctaccgtg ggagcgccca
     3781 ggatgtgctc gaccgctact accaggcagc tcgctcccac gaggccgaga ttgtggtgcg
     3841 catcaccgcc gattgtcccg tgattgaccc agggttgatc gacgagacgg tgaaggcact
     3901 atttgagcct tcaccagtca gcagtcagga accagcaatt gcttacgact tcgccgccaa
     3961 ccggctgcct ccgccctggg ggagaaccta ccccatcggt ctggatgtgg aggcttgcac
     4021 gttcgctgga ttagagacag cctgggtgga agctcgccag ccccatcagc gcgagcacgt
     4081 aatgcccttc ttttacgaaa acccgcagcg ttttcgcatc tgcctgctca atcaccacac
     4141 cgacctgagc gcctatcgct ggacggtgga cagcgccgaa gatcttgagc tattgcgcca
     4201 gatctacagc cgcttcggcg ggcgagacga tttctcctgg ttggaagtgc tggccctatt
     4261 tgagcgcgag ccagagctgt tgcagattaa cgctgtggtg atgcataaag attaccgcga
     4321 gatcgatgag cgataagagc actttgagca taaaatcggt tttcgataaa ggcctggtga
     4381 tttcaatcgc cacatggctg ctgctctccc tggtcttcgg cctggcatac acccaatcgc
     4441 acctgtacaa cgacaaccag aataccaagt ttctacacgg gctggcgatg ggcggcatgg
     4501 gtttgctcaa agaggactgg ctggcaaaca ccgccgatcc gctgccggcc ttcagcatgc
     4561 tcgtcagctt cacctttgcg tttctcgccg agagactctt ctacgtctac ttcgccttgc
     4621 tgatgggcgt gtatgcctac agcatcctgg ggatcgcctc aagcctgtat ggcatgaacc
     4681 gttcgccggc aaaatacctc gcctactttg tggtgatcct ggcaatccac tcaatccagg
     4741 ggcagatatt tgccagaaag gtgctggatt tcgatgtaga gatcctgcat tttggcctgg
     4801 caggtcagta cctgctcggt ttagattttc aaaatagttc ttttggcgtc ttcctgctgc
     4861 tctctatgca ggccttcgtg cagcgaaaaa ctgtatgggc tatcctatgg ctctccctgg
     4921 ccagtagatt ccattcagcc tacctgttca gcgccgccct gatgacgcta gcttattag
//
LOCUS       DBNGSX010000399         3587 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1075154, whole genome shotgun sequence.
ACCESSION   DBNGSX010000399 DBNGSX010000000
VERSION     DBNGSX010000399.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1075154"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..902
                     /locus_tag="AC16CB_11920"
     CDS             144..902
                     /locus_tag="AC16CB_11920"
                     /inference="COORDINATES: protein motif:HMM:NF016176.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HIR3343222.1"
                     /translation="MRVKILTLSIIALLMAACSAAAPQPEASSPTQAIQPTQPPSAPP
                     TEAPTATTELSPTDIPAAEPSSTEAPSAGGAMAYQIIPGDSQLKYEVGEVFFKDNNRF
                     NLAVGVTPQVTGEILVDMSAPQNSQIGTISADISQFQSDSSRRDNAIRQRFIETSRYP
                     IVTFTPTAVEGLPEAYSEGQEIPLKISGDLTIREVTRPVTFDALVKLEGDTLSGSATT
                     TILMSEFGFGPIDILGTLKTEDEAKVTLSLVARP"
     gene            1017..1805
                     /locus_tag="AC16CB_11925"
     CDS             1017..1805
                     /locus_tag="AC16CB_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011317283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIR3343223.1"
                     /translation="MFLFLSKFLPLLVYPLGLASILILVSLFIRQPRWQKGILALAFV
                     VLYLGGNGWVAHALAKSLEWRYLPPEEIPQADVIVVLAGDERSAQYPRQTPEVGEAGD
                     RVIYAAWLYQQGAAPAILFSGGSIKWLSVGSSPKDAAAYFLEMMGVPRKALWYESESR
                     NTYESAVACRKILDERGIDRVILVTSAAHMPRSVALFEQQGIEVIPAPTDYTVTQSVP
                     QHRLSGRLPSVVISLLPSTEYLEITTRMLKEYFGIFIYKLRGWL"
     gene            1817..3049
                     /gene="coaBC"
                     /locus_tag="AC16CB_11930"
     CDS             1817..3049
                     /gene="coaBC"
                     /locus_tag="AC16CB_11930"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR3343224.1"
                     /translation="MANPLKDKHIILGVTGSIACYKAVELASKLAQAGALVDVILTQA
                     ATQFVTPLTFQSVTGRRAFVEADLWGSEGHVLHISLGHVAELLVIAPASANTIAKLAH
                     GIADNFLTVTALAANCPLLVAPAMDGGMYTHPATQANLKTLQERGAIILGPVEGHLAS
                     GLQGVGRMMEPGELLGHIRLALAQTGPLRGRRVIVTAGGTHEPIDPVRAISNRSSGKQ
                     GFALAQAALDMGAQVTLITAPVSLPTPVGAQRQEVLTAQDMLEAVSEAIPHADALIMA
                     AAVADFRPADPAGQKIKREGGLPEIQLENTPDILQAVARYKSDTGYPRVTVGFAAESQ
                     ALLENARKKLEKKSLDLIVANDISASDTGFAVDFNRVTLLDAQGGVEHLPRMSKTDVA
                     EIVMQRVVAILNSLHSEL"
     gene            3046..3363
                     /locus_tag="AC16CB_11935"
     CDS             3046..3363
                     /locus_tag="AC16CB_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009555946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HIR3343225.1"
                     /translation="MIVHICQLGAWQAAQARGEYCPPSLDDEGFIHCSKPEQVLHVAK
                     RFYRDVNDLILLWIDTKKVRPEIRWEEADGEVFPHIYGALNTDAVVSVSELDLDMDLI
                     PPF"
     gene            complement(3372..>3587)
                     /locus_tag="AC16CB_11940"
     CDS             complement(3372..>3587)
                     /locus_tag="AC16CB_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase WbuB"
                     /protein_id="HIR3343226.1"
                     /translation="IRQVVEVAGSGVYVPPGDAQALANAIRELAANPAMRQSMGLAGR
                     RCVEEAFSRELLAEKMAKIIEEMRDRG"
BASE COUNT          852 a         1024 c          905 g          806 t
ORIGIN      
        1 cgatgccccc cctgccacgt atccaactgc gtaacttcta taattgttaa agcctgatta
       61 gaatttctga tcccgcaatc catcatgttg ttggatgcgt aaaaataggt gattaccaaa
      121 tccagaaaag aaagaggaat acaatgcgcg ttaagatatt aacgttatca attattgctt
      181 tgctgatggc agcctgcagc gccgcagccc cacaaccaga agctagtagc cctacacagg
      241 ctattcaacc gacacaacca ccatccgccc caccaacgga ggctcccacc gcaacaactg
      301 agctttcccc tacggatata ccagcagctg aaccttctag tactgaagca ccatcggctg
      361 gcggcgcaat ggcttaccag atcatccctg gagattcaca actgaaatac gaggtcggtg
      421 aagtcttctt taaagataac aaccgtttta accttgccgt tggggtcaca ccccaggtta
      481 cgggcgagat tctggtcgat atgtccgcac cgcagaacag ccagattggc accatcagcg
      541 ccgatatcag ccagtttcag tcggatagct caaggcgcga taacgcaatc cgacaacgat
      601 ttatcgaaac ttcccgctat cctattgtca ccttcacccc gaccgcggtc gagggcttgc
      661 ccgaggcata ttccgaaggt caggaaatcc ccttgaaaat tagcggtgat ctaaccatcc
      721 gggaggtcac ccgcccggtc actttcgacg ccctggtgaa actggaaggt gacaccttga
      781 gtggttccgc cacaaccacc attctaatga gcgagtttgg atttggaccg attgatatcc
      841 tcggcacttt gaaaacagaa gacgaagcca aagtaaccct atcgctcgta gcaagaccat
      901 aaatatctcc tagtgcgcgg atgtatgtgt atatggggtc tgagcaggtg cagggttgcc
      961 cgcacagacc ccgttttttg ctattaattt atctccaaat gcattatgat taacccatgt
     1021 ttctttttct ttcaaaattt ctgccactcc tggtctaccc attagggctt gcctctatcc
     1081 ttatcctagt gtctcttttc atccgccaac ccagatggca gaaagggatt cttgccctgg
     1141 cctttgtggt gctctatcta ggtgggaacg gttgggttgc acatgccttg gcaaaatctc
     1201 tagaatggcg ctatttaccc cctgaagaaa tccctcaagc agatgtgatt gtcgtcctcg
     1261 caggggatga gcgttcagca caatacccgc gccaaactcc cgaggtgggt gaggcgggag
     1321 atcgcgtcat ctacgctgcg tggctgtacc aacagggcgc tgctccagct atcctgttca
     1381 gcggcggttc catcaagtgg ctctctgtgg gctctagtcc aaaggatgcc gcagcctact
     1441 ttttggagat gatgggagtc ccgcgcaagg ccttgtggta tgagagcgaa tcacgcaaca
     1501 cgtatgaaag cgcagttgcc tgccgtaaaa tcctcgatga aaggggcata gaccgagtga
     1561 tattggtaac ctctgctgca cacatgcccc gatcagtagc cctttttgag cagcagggga
     1621 ttgaagtcat cccagcgcct accgattaca ccgtcaccca gtcagtccct caacaccgat
     1681 tatcaggccg gttgcccagt gtggtgatct cgctactccc cagcaccgaa taccttgaaa
     1741 ttactactcg catgctaaag gaatatttcg gcatctttat ttacaagcta cgtggttggt
     1801 tgtaatataa gcatatatgg ctaatccact caaagataaa cacatcattc tcggggtcac
     1861 cgggtcgatt gcgtgctata aagccgtgga gctggcgtcc aagctggccc aggctggtgc
     1921 gctggtggat gtcatcctta cccaggcagc gacccaattc gtcaccccgc tcactttcca
     1981 gtcagtcact ggacgacgcg cttttgtgga agccgatcta tggggcagcg aaggtcacgt
     2041 gcttcacatc agcctaggcc atgtcgccga actactggta atcgcacccg cctcggcaaa
     2101 taccatagcc aagctggcgc acggcatcgc cgataatttc ctgacggtga ctgcgctggc
     2161 agccaactgc ccactgctcg ttgcaccagc tatggatggc ggtatgtaca cccacccggc
     2221 gactcaagcc aatctgaaga ccttgcagga gcggggcgct atcatccttg gcccagtcga
     2281 gggtcacctg gcatccggat tgcaaggagt cgggcgaatg atggagccgg gagaattgct
     2341 tggtcatatc cgcctggcac tggcgcagac cggtccccta cgtgggcgca gagtgattgt
     2401 cactgcaggc ggcacgcacg agcccatcga cccggtaaga gctatcagca atcgctcatc
     2461 aggcaaacag ggctttgccc tggcccaggc tgccctggat atgggggcgc aggttacttt
     2521 gataacagct cccgtctctc tgcccacgcc agtgggggcg cagaggcagg aggtgctcac
     2581 agcccaagac atgctggaag cggtatcaga ggctattccg catgccgacg cactgattat
     2641 ggcagccgct gtggccgatt ttcgacccgc tgaccccgcc gggcaaaaga tcaagcgcga
     2701 aggtggtctt ccagagattc aactcgaaaa cacgcctgat atcttgcaag ctgttgcccg
     2761 ctataaatcc gacaccggtt acccgcgggt gacggtaggt ttcgctgccg aaagccaggc
     2821 tttactggaa aatgcccgca agaagctgga aaaaaagagc ctggatttga tcgtcgccaa
     2881 cgatatctcc gccagcgata ccggcttcgc ggtggacttc aaccgcgtga ccttgctaga
     2941 cgcccaaggt ggagtagaac acctgccccg catgagcaag acagatgtag cggagattgt
     3001 aatgcaacgg gttgtggcga tattaaattc tttgcattca gagttatgat cgtgcatatc
     3061 tgccagctcg gggcatggca agcagcccaa gcccgcggag aatactgccc accctccctg
     3121 gacgacgaag gtttcatcca ctgctccaaa ccagaacaag tcttgcatgt tgcgaagcgg
     3181 ttttaccgcg atgtaaatga tttgatctta ttatggatcg acaccaaaaa agtgcgccca
     3241 gagatccgct gggaagaggc agatggggag gttttccccc atatttacgg agcactcaat
     3301 acagacgcag tggtctctgt gagcgaactg gacctagata tggatcttat cccgcccttc
     3361 taaaacctct attaacccct atccctcatc tcttcgataa ttttagccat cttctcagcc
     3421 agcaactcgc gcgaaaaggc ttcctccacg cagcgccgac cagccaaccc catcgactgt
     3481 ctcatggcgg ggtttgcagc caactcgcgg atggcgttcg ctaaggcttg cgcatctccg
     3541 ggtgggacgt aaacaccgct gcctgccacc tcgacaacct gccggat
//
LOCUS       DBNGSX010000400         2499 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1006489, whole genome shotgun sequence.
ACCESSION   DBNGSX010000400 DBNGSX010000000
VERSION     DBNGSX010000400.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1006489"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="AC16CB_11945"
     CDS             <1..978
                     /locus_tag="AC16CB_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343227.1"
                     /translation="IFIAPFAIYLLVRWYQRNKDMPMGVRLKHIVIPFAISILVLAAY
                     FVPFLISLPSTTKSYWTWRITGINVREKRAISSLLNFELYNPIFTIYIYAIFALLSLP
                     KIKTTYPLILWFVLPWSVLELVIFDPGTHIYTYIIPAIILVAFGLEILVKIILKILGN
                     IWGMRLNIAWLTLLFAWLAGISHLIFVDHTPEYPYEERVILFWTIGGPRPVHDYRPWA
                     YGFPYYRGWEEIRDFVTTTENNGYYTTNENTSISIYYVPFTYFADRAGHYIYIHAPQS
                     FESKDRDPKPKYWRKNYKPVKVLEYNDKVIAEIYYMPAGSLEQIWQAGY"
     gene            1013..>2499
                     /locus_tag="AC16CB_11950"
     CDS             1013..>2499
                     /locus_tag="AC16CB_11950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR3343228.1"
                     /translation="MNDKIKLIPILFLIFVTLWLRLVNLGYSDYQGDEIKAMWRPAPG
                     QSGIDYLYTQKKGPTQFLVSYLVRFIDPTYSNEFLLRLPFTLAGILGIYFFYRLVKMH
                     FGEKIALYSTLLLSINGIFIGLMRIVQYQPFVILFSVLTLYCFSLASQQEGWKFRGIT
                     LGVTFWTVALFSHYDAIFIAPFALYLLYRWYTQNNDLPVRVRLKHLIIPFAISTLLLA
                     IYFVPYLLSLPSNIKDYWTERITGEGGTDKRARSSLVSFNLYNPLLATYVYGFFGLLS
                     LPKLKKNIPLILWVALPWAVLELAVFDPGTHIYTYIIPVIILIAFGMEVLEDVFVKTL
                     GAVWGMRSNILWLCLLFGSLAAISHLIFVDHTPEYPYEKRGILFWTIGGADTVKESKL
                     WNFGFPYYRRWEDIREFVSTSENTGYYATNENVSISVYYVPYTYFVERAGHYIYIHNP
                     QSFKTWDGRDKIQYWRANHDPVKVFEYNGRVVAEVYFMPPGSLEE"
BASE COUNT          598 a          665 c          548 g          688 t
ORIGIN      
        1 atttttatcg cccccttcgc tatctacctg ctggtgcgct ggtaccaacg caacaaagac
       61 atgccaatgg gagtgcgtct gaaacacatt gtgattccct tcgccatcag catcctagtc
      121 ctagcagctt acttcgtgcc tttcctaatc tcgctgccca gcaccactaa atcctactgg
      181 acctggcgga tcactggcat aaatgttaga gagaagcgcg caatcagctc gctgttaaat
      241 tttgagctat acaaccctat ctttacgata tatatttatg caatctttgc gctcttatct
      301 ctacctaaga tcaagaccac ttaccctctg attctatggt ttgttctccc ttggtcagtt
      361 ttggagttag tgattttcga tcctgggacc cacatctata cctatattat ccctgctatt
      421 atcctggtcg ccttcggact agagatactg gtaaagatca tactcaagat cttgggaaat
      481 atttggggga tgcggctgaa tatcgcatgg ttgacgctcc tcttcgcctg gctagcgggt
      541 atctctcacc tgatcttcgt cgaccacaca ccggaatacc cctacgagga acgcgtcatt
      601 ctgttctgga ccattggagg gcctcgcccg gtgcatgatt acagaccgtg ggcatatggc
      661 ttcccctact accggggctg ggaggagata cgcgattttg tcaccacaac cgaaaacaat
      721 ggatactata ccacgaatga aaatacatca atttctattt attacgttcc ctttacttac
      781 ttcgcagatc gagccgggca ttatatttat attcacgcac cgcaaagctt tgagagcaag
      841 gatcgagatc caaaacccaa gtactggcgg aaaaattaca agccggtgaa agtgctggaa
      901 tacaatgaca aggtaatagc ggagatctac tacatgcctg ctggtagctt agaacaaata
      961 tggcaggcag gatactgaaa tgttgaattc tgtgcgatcc ttaagatatt tcgtgaacga
     1021 taaaatcaaa ctgatcccca ttctgttcct gatcttcgta accctctggc tccggctggt
     1081 caacctgggc tattccgatt accagggtga tgagatcaaa gctatgtggc gccccgcccc
     1141 gggacagagc ggaatcgatt acctatacac ccaaaagaaa ggcccaactc aattcctggt
     1201 tagctacctg gtcaggttca tcgaccccac ttattccaat gaattcctgc tgcgcctgcc
     1261 cttcaccctg gctggtattc taggtatcta tttcttctat cgcctggtca agatgcactt
     1321 cggcgagaag atcgccctgt attccaccct gctgctctcg atcaatggca tctttattgg
     1381 cctgatgcgc attgtccagt accagccctt cgtgattcta ttttcagtgc ttacgctata
     1441 ctgcttctcg ctggcatccc agcaagaagg ctggaaattt cgggggataa cgctcggggt
     1501 gaccttctgg acggttgccc tattctcgca ttacgacgca atcttcatcg cccccttcgc
     1561 tctctacctg ctctaccgtt ggtacaccca aaacaacgac ttgcctgtcc gagtgcgact
     1621 gaagcatttg atcatcccct tcgccatcag caccctgctg ctagcgattt acttcgttcc
     1681 atacctgctc tcccttccga gcaatatcaa agattactgg accgagagga ttaccggtga
     1741 aggcggaacc gacaagcgtg cacgcagctc gctggtcagc ttcaatctgt acaacccact
     1801 cctggcaaca tatgtctatg gattcttcgg gcttttatcg ctcccgaaac ttaagaaaaa
     1861 tatccccctc atcttgtggg ttgcccttcc ctgggctgtt ttggagctgg ctgtcttcga
     1921 cccaggcaca catatctata cctatatcat acctgtcatc atcctaattg cctttggcat
     1981 ggaggttcta gaggatgttt ttgtcaagac tttgggtgca gtctggggga tgcggtcaaa
     2041 tatcttgtgg ctgtgcctgc ttttcggctc cctggcagct atctctcacc tcatctttgt
     2101 cgatcacaca ccggaatacc cctatgagaa acgcggcatc ttgttctgga ccataggtgg
     2161 tgcagatacg gtaaaagaga gcaagttatg gaatttcggc ttcccatact accggcgctg
     2221 ggaagatatc cgcgaatttg tcagtacgtc agaaaacacc ggttattatg caacgaatga
     2281 gaatgtgtct atctctgttt attatgtacc ctatacgtac tttgttgaaa gagccgggca
     2341 ctatatctac attcacaacc cacagagctt caagacctgg gatggccggg acaaaattca
     2401 atattggcgc gcaaaccacg atcctgtgaa agtctttgag tataatggcc gggtagtagc
     2461 agaggtttac tttatgcccc caggcagcct ggaagagat
//
LOCUS       DBNGSX010000401         2454 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1075332, whole genome shotgun sequence.
ACCESSION   DBNGSX010000401 DBNGSX010000000
VERSION     DBNGSX010000401.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1075332"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="AC16CB_11955"
     CDS             complement(<1..185)
                     /locus_tag="AC16CB_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HIR3343229.1"
                     /translation="MKRAVSISLGSSDRDKRVVVQLKNELIEVERIGTDGDVGKAIRL
                     FTELDGKVDVLGVGGVDL"
     gene            320..1147
                     /locus_tag="AC16CB_11960"
     CDS             320..1147
                     /locus_tag="AC16CB_11960"
                     /inference="COORDINATES: protein motif:HMM:NF025615.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HIR3343230.1"
                     /translation="MKNGITQNYLRRHIAIPSEHGSWVFVLSPLLIGLFAGGRWVTGS
                     FYLILAAMAAFLIRQPVTIAVKAYSGRRSKRDLPAAWFWIIAYGLIGLVAFALLTAQG
                     FGSLLLLALPGIPVFIWHLYLVSKRAERGQAGVEMVASGVLALAAPAAFWVGVGEPDP
                     SGWWLFALVWMQSAASIVYAYLRLAQRELASVPESIIRLRMGRRALLYTTFNLAWVIF
                     FSIFRVFPPLLPLPYLLQWVETLFGTLRPAVGVKPTSIGIRQLLISTLFTVLFIMTW"
     gene            1288..1629
                     /locus_tag="AC16CB_11965"
     CDS             1288..1629
                     /locus_tag="AC16CB_11965"
                     /inference="COORDINATES: protein motif:HMM:NF021221.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HIR3343231.1"
                     /translation="MTDQPAEQTTNDITSDDKLWSLLGYILSPIVPIIVLLLEDKKSR
                     PFIRYHNMQALVLGVVNIILSLILGWTLVLACVPFLIWVVMVYWGIQAYQGQWVKIPV
                     LTDFVKNQGWA"
     gene            1717..2154
                     /locus_tag="AC16CB_11970"
     CDS             1717..2154
                     /locus_tag="AC16CB_11970"
                     /inference="COORDINATES: protein motif:HMM:NF021221.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HIR3343232.1"
                     /translation="MDVNTGTGSPESLSPSDERTWAMLAHLTVLANLVTGFLGTAAAL
                     VIYLVYKERSRYVAYHSMQAFIFQLVWWIGGGALTGIAWTFTGISSIALIGLLCIPLA
                     CLVSLVPLGSIVYGIIGAIQCSQGQDFRYWLVGDWVRGTLTGE"
     regulatory      2234..2338
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
BASE COUNT          547 a          676 c          589 g          642 t
ORIGIN      
        1 aggtcaaccc cgcccacacc caacacgtca acttttccgt ctagttccgt aaacaaacgg
       61 atagcttttc ctacatcacc atccgtgccg atccgctcta cttcgatgag ttcatttttt
      121 aactgaacaa caacgcgttt atctcggtct gagctcccca agctgatgct gacggcacgt
      181 ttcataacgc tccaaaagga cagatctata tcttgccatt cctagaatgt tgctagttga
      241 ctggaagatt atattacaag tcagaccaat ctcacccctc gtaagggact atcatgccaa
      301 atcagctata attctctccg tgaaaaatgg catcacacaa aattacttgc gccggcatat
      361 tgccattcca agcgaacatg gatcctgggt attcgttcta agtccgctat taattggtct
      421 gttcgccgga gggcgctggg taactgggtc attctatctc atcttagctg ctatggcagc
      481 cttcctcatc cgccagccag tgaccatcgc ggtaaaagcc tacagcggtc gccgctcgaa
      541 acgcgatcta ccagcggcat ggttctggat catcgcttat gggctaattg ggttggtagc
      601 atttgccttg cttactgccc aaggatttgg ctccctgttg ctcttagcct tgcctggcat
      661 accagtattt atctggcatc tctatttggt tagcaagcgt gctgagcgcg gccaagctgg
      721 cgtggaaatg gtcgccagcg gtgtgctggc attggctgca ccagccgcct tctgggtggg
      781 cgtgggcgag cctgacccat ccggctggtg gcttttcgcc ctggtttgga tgcaatcggc
      841 agcctcgatc gtatatgcct acctgcgcct ggctcagcgg gagctggcct ctgtccccga
      901 atctatcatc cgtctgcgca tgggacgcag ggcattgcta tacaccacct tcaatctggc
      961 atgggtaatt ttcttctcca tcttcagggt attcccccca ctgctccccc ttccttacct
     1021 gcttcaatgg gtcgagacat tgttcggtac gctccgaccg gcagtcggtg ttaaacccac
     1081 tagtattggg atacgccagt tgctcatcag caccttgttc accgtgctct tcatcatgac
     1141 ctggtaatcc caccttgcaa acctgatagc aaccctaacc aacttttggg gttattagat
     1201 agagagatca cttgctttgc attcaacctc ttgtactatg cttgcacagt gctccaaaac
     1261 accatctttt ccataggagg caatcaaatg accgaccaac ccgccgaaca gaccaccaac
     1321 gacataacca gcgatgacaa gctttggtcc ttgctgggct acattctttc ccctatcgtt
     1381 cccatcatcg tgctcctcct tgaggataag aagagtcggc cttttatccg ctaccacaac
     1441 atgcaagcgc ttgttcttgg agttgtcaat attatcctct ctctaattct cggatggacg
     1501 ctggtccttg catgtgtacc cttcctgatt tgggtcgtca tggtctattg gggcattcag
     1561 gcataccagg ggcaatgggt taaaattcct gtcctgaccg atttcgtaaa gaaccagggt
     1621 tgggcataat tatttgatat atttttcagc cccgtcttca tacccattcc gaagacgggg
     1681 ctatttgtgt cgcaaatctc aaaacaagga gaaaaaatgg acgtaaatac cgggacaggt
     1741 tcgcctgagt cactctcccc atcagacgaa cgcacctggg ctatgctggc tcacttgacc
     1801 gtgctggcca acctggtcac tgggtttttg ggcacggctg ctgcgctggt tatttacctg
     1861 gtttataaag aacgctcgcg ttatgtcgca taccattcca tgcaggcttt cattttccaa
     1921 ttagtctggt ggataggagg cggcgctcta acgggtattg cctggacttt cactggcatc
     1981 tcatcaatcg ccctgatcgg gttattgtgc attccattgg cttgcctggt cagcttagtg
     2041 ccactgggct cgattgtgta tggcatcatt ggcgccatcc agtgcagcca gggacaggat
     2101 ttccgctatt ggctggtggg cgattgggtg cgtggcacac taacgggtga gtaacccgac
     2161 ccccttacat aggcggggac gaattacact tgacttttag cgtactcacc cttagaatga
     2221 tggcgaacaa tttaacggga tcagggaaga gactcgggta aaacgagcac cgaaggggca
     2281 aatctggcag aaagcaatgc caggtgaaac tctcaggtaa aaggaccctg atgctggaac
     2341 cctctggaaa gtctggcagg ttatcacaac ccgcagtacg ctagcctgca caccgacggg
     2401 gcaagcgcga cacccagcgc gaatctctca ggttcaagac agaggcgcac gcaa
//
LOCUS       DBNGSX010000402         2329 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_382141, whole genome shotgun sequence.
ACCESSION   DBNGSX010000402 DBNGSX010000000
VERSION     DBNGSX010000402.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_382141"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1212)
                     /locus_tag="AC16CB_11975"
     CDS             complement(166..1212)
                     /locus_tag="AC16CB_11975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012401.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HIR3343233.1"
                     /translation="MKSHFSSLNTITTEPYNAETPHAALMKDITPTDLFYVRNHFAVP
                     IIDAGDWALELGGAVEKPLQFSLLEIQKLPPKTLNVILECAGNGRSTLNPAPNGTPWN
                     LGAVSQARFIGTSLNNLFKELTISRDAIEMLFVGADQGTTRTKQTTTYARSLPLEVAL
                     HPDTMLAWEMNGEPLPLQHGYPLRLVVPGWYGMASVKWLRQISLLRERFEGFFQKDEY
                     VYVGESGIPDGTPVTSMRARALIVEPSPEAILPAGEVQLAGIAWSGEGSITRVELSFD
                     DGATWLEADLETARYPYSSQRWKFNWNMDIPGQYAITVRATDSRGNFQPLTQRWNKGG
                     YGNNKAQQVTVSIN"
     gene            complement(1351..2268)
                     /locus_tag="AC16CB_11980"
     CDS             complement(1351..2268)
                     /locus_tag="AC16CB_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3343234.1"
                     /translation="MTSKVVKKRGQYILAFDGSEHARAAVSFMQDFIIQQGEKNKSTV
                     VLLAVFTPRQIGDLTPLEQALDQTHIFFKDKGIRSKPELVLGYPGEKILEYAEELNPK
                     MIVVGAKGLRATLGILLGGVAQEVVESAQCPVMVIRAPYQVFQRILLVADGSTYSQKA
                     IEYVAKFYLTPLASIDIINVLPPIPIKPVPDFITHSWPAGPEVIPQFTPEMSEEESAF
                     LREEEKEGQKILDGAQRPLQKKGKTVQINQVLVRGDAASEIIQYAKDNKIDLIVTGSH
                     GMGAIRSILLGSVTRKLVHYANSSVLVVK"
BASE COUNT          585 a          531 c          567 g          646 t
ORIGIN      
        1 tagaaagttt tggtgaagct agcttatttg tcatatggtt attataggat atttattatc
       61 aatagcagtg ttcctcgttg tggaatgcta tgcttgatga tgactttagc cccgcactca
      121 gtcctaataa agggaaaaac gaaaatttca atcttggggg gaatttcaat taattgatac
      181 agtaacctgt tgcgctttat tattgccata accacccttg ttccagcgct gggtgagtgg
      241 ttgaaagttc cctcgggaat cggtggcgcg tactgtaatg gcgtattgtc caggaatatc
      301 catattccag ttgaatttcc aacgctggct gctgtatggg tatcgagcgg tttcgaggtc
      361 tgcttccagc caggtagcgc cgtcatcaaa gctcaattca actcgggtga ttgatccttc
      421 accgctccag gcaattccgg caagttggac ctcgcctgct gggagaattg cctccggcga
      481 tggttcaaca ataagggcgc gtgcacgcat gcttgtaacc ggtgttccgt ctgggatgcc
      541 ggattcgccc acgtatacgt actcgtcctt ctgaaagaaa ccctcaaatc gttctcgtaa
      601 cagcgaaatc tgccgcagcc attttaccga tgccatacca taccagcccg gaactacaag
      661 gcgcaaggga tagccatgct ggagaggcag tggctcgcca ttcatttccc aggcaagcat
      721 ggtatctgga tgtagagcta cttcaagggg taagctgcgt gcatatgtcg ttgtttgttt
      781 ggtgcgggtt gttccttgat ctgcccccac aaaaagcatc tcaatcgcat cacgggaaat
      841 tgtaagctct ttgaagaggt tattcaatga ggtcccaatg aagcgcgcct ggctcactgc
      901 acctaggttc caaggagtac cattgggagc gggattcaag gttgaacggc cgttaccggc
      961 acactcaagg atgacgttca gggtttttgg gggtagcttt tgtatctcga gaagtgagaa
     1021 ttggagcggt ttctccacag cgccacccaa ttccaacgcc caatcgccgg catcgatgat
     1081 tgggacggca aaatgattgc gaacataaaa caaatcagtg ggagtgatgt ccttcatcaa
     1141 agccgcgtgg ggagtttcgg cattataagg ctcggttgtt atagtattca agctggagaa
     1201 atgtgatttc atggagttca cctaaagaga gatatgtacc cattatagtc catattaatt
     1261 ttgagatttt cggggagaaa tcttatcaac gcacgataat gcattctgta aacgtaatgg
     1321 cagtttacag gatgaaattg ggaggcagga ttactttacg accaatacag agctattcgc
     1381 gtagtgtacc agcttgcggg tgacgctgcc tagtaatata ctcctgattg cgcccatgcc
     1441 gtgcgaaccc gtaacaatca ggtcaatctt attgtctttt gcatattgga taatctcgct
     1501 ggcagcatca ccgcgcacaa gaacttgatt aatttgcact gtcttcccct ttttctgcaa
     1561 tggcctttga gctccatcca atattttttg accttccttc tcttcctcgc gcagaaaagc
     1621 cgattcttct tcagacatct ctggggtgaa ttgggggata acttcaggac ctgccggcca
     1681 ggaatgggtt ataaaatctg ggactggctt tatggggata ggtggtagca cattgataat
     1741 atcaatacta gccaatggcg tgagatagaa cttggccacg tattctatgg ctttttgact
     1801 ataagtggat ccgtcagcaa ccagcaatat gcgttgaaat acctggtatg gcgcccggat
     1861 aaccattacc gggcactggg cggactcgac aacttcttgg gctacaccgc ccaataaaat
     1921 cccaagcgtg gcacgcaagc ctttcgctcc gacgacgatc atcttcggat ttagctcttc
     1981 agcgtattca aggatttttt ctccaggata tccaagaacc agttcgggtt tagaacgaat
     2041 gcctttgtct ttgaaaaaaa tgtgggtctg gtcaagtgcc tgttcgagcg gcgtcaagtc
     2101 tccgatctga cgaggagtaa aaaccgccaa caacacgacc gtgcttttat ttttttctcc
     2161 ttgctgtatg atgaaatctt gcataaatga gactgcagcg cgggcatgtt cagagccatc
     2221 aaaagctaga atatattggc cacgtttttt gacaaccttg gaggtcatag ctgcactcct
     2281 atataatttt tcttataaaa atagacctgc ctggaatgac cctgagatc
//
LOCUS       DBNGSX010000403         6822 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1283175, whole genome shotgun sequence.
ACCESSION   DBNGSX010000403 DBNGSX010000000
VERSION     DBNGSX010000403.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6822)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6822
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1283175"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="AC16CB_11985"
     CDS             <1..776
                     /locus_tag="AC16CB_11985"
                     /inference="COORDINATES: protein motif:HMM:NF019561.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HIR3343235.1"
                     /translation="VVDVRVSLDVSNANDTVRNNVTVQPVDQNGKPVAGLSVTPSTVE
                     LIQVVSLLGGYRNVVVKVTTAGQVAQGYWLTNVSVTPPNVTVFSTNPRLVNELPGFVE
                     TNPLDLTDLSDDIDMRTTLNLPEGVTLAGEESVLVRLSIAALEGSLPITLPLEVVGLS
                     PEYQAKLSPDTVDLLLTGPLPLLNNLTPGGIRVSVNLSGLEPDVHQVPPKVDLLPPQV
                     SVASIIPENVEVTIEIAPPETITPTGQGSPIPATVTPTP"
     gene            787..2160
                     /gene="der"
                     /locus_tag="AC16CB_11990"
     CDS             787..2160
                     /gene="der"
                     /locus_tag="AC16CB_11990"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR3343236.1"
                     /translation="MPKPVVVLVGRPNVGKSTLFNRLAEERLAVVDETPGTTRDRLMA
                     EAEWAGVAFDIVDTGGIDPSLMASGRKKEPLSIGSADYIGQIRAQAEMAIQEADAVLF
                     VTDAEDGVTPADREVAQILRRHQREVDGESWPPVFLVVNKADSEARRAQALQFYELGM
                     GDPHPISALHGTGTGDLLDVLVASFREPGEEAEDDTVKIAIVGKPNVGKSSLLNRLVG
                     QERAIVSPIPGTTRDAVDEQIMVGEIPVTLIDTAGIRRRGRIEPGVEKYSVLRSLRAI
                     ERADVSLLVVDATSGMTAQDTHIAGFILDAWKSAVVLVNKWDAIPKDSYTMDHFTRQV
                     RQELNFMDYVPVLFISAKTGQRVEQVLPLALRVQEERLVHISTSDINRIIQDAQRRHA
                     APSHAGRQLKMYYGTQVRSDPPTFMIYVNDPKLAHFTYRRFLENRVREAYGFLGTPIR
                     IVLKPRR"
     gene            2214..2816
                     /gene="lepB"
                     /locus_tag="AC16CB_11995"
     CDS             2214..2816
                     /gene="lepB"
                     /locus_tag="AC16CB_11995"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR3343237.1"
                     /translation="MDNNIDSITIETADDQAAIPQTKTKIVRFLVDIVETLVFSLLLF
                     AGINALTARIRVDGMSMEPTLHSGEFVIVNRIAYRFGQPAIGDVIVFLFPRDPTQEYI
                     KRVIGLPGDEVVISNSQVQVNGQALNEPYIAAPPKYESQWTVPEGSLFVLGDNRNNSS
                     DSHTWGPVPLENVIGKAVFVYWPPVEWGVVEHITPVAGLP"
     gene            2934..3215
                     /locus_tag="AC16CB_12000"
     CDS             2934..3215
                     /locus_tag="AC16CB_12000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HIR3343238.1"
                     /translation="MAGEDTNEANKFVALCPECQTGVLRLRSLTYFTWLNTELITVPN
                     FPAWVCDVCGRREYDPRAIKWLNTLLNPEAGRSQNRPRSMPPRVDRPRP"
     gene            complement(3227..3760)
                     /locus_tag="AC16CB_12005"
     CDS             complement(3227..3760)
                     /locus_tag="AC16CB_12005"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3343239.1"
                     /translation="MTFQKISSSRIYRGRVFDLHQDEVRLPNGRVVQLDIVQHPGAVV
                     LVPVDAQGNIWFVRQYRHAAGTEMLELPAGTMERGEDIESCAYRELREEIGMAAGEVL
                     KIGEFFMAPGYSTEYVYIYLATSLQPAPLPGDDDEFIHVERMAAGEAFELAEKGQIPD
                     AKTVAALSLARPHIQRF"
     gene            complement(3852..4559)
                     /locus_tag="AC16CB_12010"
     CDS             complement(3852..4559)
                     /locus_tag="AC16CB_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR3343240.1"
                     /translation="MKSKRFPLLLVTILLVIFISACERPASVAPESVGSPTVPEGGFP
                     LPGVTEDVMGQLESFATQTAFALAGGVTAPETPTGEVAPAATSTPEAAAAAAPTATAT
                     TAPQAASSSSCSSIVIPTATPGQSSSWTLQRGEHPYCIARRFDVNPVDLLNASGLNSG
                     SSYAVGTKLTIPKSARGFPGKRSLKSHPDTYTVGSRDSIYSIACEYGDADPYAIALAN
                     GLSAPYKLTNGQQLCIP"
     gene            5032..5694
                     /locus_tag="AC16CB_12015"
     CDS             5032..5694
                     /locus_tag="AC16CB_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343241.1"
                     /translation="MYLTSTANVARAQQETPTPSPSEVPTGTPISPRPLGTPELSIRS
                     PLPGQAVQGSVPIAGSSAVTGFVSAEVSFTYAGDQTGTWFFIGDTSDAVVDGTLAQWD
                     TSSITDGDYDLRLRVLREDGSYLDAVILGLRVRNYTPIETATPTPVTPTATQMPGELT
                     ATSTAIPMSLPSVTPLPTNPAEITGQDIAWGLGKGALAVIGLFALMGVYKLVNEIGNK
                     EQ"
     gene            5715..6335
                     /gene="pth"
                     /locus_tag="AC16CB_12020"
     CDS             5715..6335
                     /gene="pth"
                     /locus_tag="AC16CB_12020"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR3343242.1"
                     /translation="MTSQRSLDMNQPATFLIIGLGNPGRDYQGNRHNIGFRLLNHLAE
                     RLGVGFSRLESKSLVTKADYLGHRLILAKPQTYMNLSGQAVESLVRFYKIPLGQVMVV
                     FDDVDLPFGALRLRPAGGSGGQKGMQSILDRLGTQEIPRLRLGIDRPPGRMEAADYVL
                     QDFSKNEVELLPGILDRAADAVLTYVRNGLDAAMNQFNPQNSDDQG"
     gene            6340..>6822
                     /locus_tag="AC16CB_12025"
     CDS             6340..>6822
                     /locus_tag="AC16CB_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343243.1"
                     /translation="MSMQLDAILNTVMETLGQRTSYLLGQVLPSFDLRRAARLPVLVS
                     MYRMTNQPVLLITDRADHALTLFDELSLWAPNAKRLLIPEPTSLFYEKAPWGENTRRE
                     RLVALTTLAAYHVPGTTPSGNDSLNHAILVAPARALMARTLPLREFLKSTRSLRPGQN
                     A"
BASE COUNT         1597 a         1712 c         1856 g         1657 t
ORIGIN      
        1 aggtggttga tgtaagggtt tcattggatg ttagcaatgc gaatgatacc gtcagaaata
       61 atgtcaccgt acaaccggtt gatcaaaatg gcaaacctgt tgcgggcttg agtgtaactc
      121 cgtcgacagt tgagctgatt caggtggtga gtttattagg cggttaccgc aatgtggtcg
      181 tgaaagtgac cacggcgggc caagtggctc agggatactg gctgactaat gtatcagtca
      241 caccacccaa tgtcactgtg ttctccacca atcctaggct ggtaaatgag ttacccggtt
      301 ttgtggagac gaacccattg gatttgaccg atttgagtga tgatatcgat atgcgtacga
      361 cgctaaacct gcccgagggg gttactttag cgggcgagga gagtgtgctg gtacgcctga
      421 gcatcgctgc gctggaggga agcctaccca ttacattgcc cctagaggta gtcggtctgt
      481 cacctgaata ccaggcgaaa ctgtcaccag ataccgtgga tttactcttg acgggtccat
      541 tacctctgtt gaacaacttg acacctgggg ggatccgcgt ctcggttaac ttaagtggtt
      601 tggagcctga cgtgcaccag gttccaccga aggtggattt gttaccccct caagtcagtg
      661 tcgcttccat aatacctgaa aatgttgaag tgacaattga gattgcccct cctgagacca
      721 taacgcctac gggtcagggt tcacccatac cagctacagt cactccgaca ccataaggaa
      781 tatgtgatgc ccaaacccgt tgtggtattg gtcggacgcc cgaatgtagg gaagagcacc
      841 ttgtttaatc gtctggcaga agaacgcctg gcggtggtcg acgagactcc tggcaccact
      901 cgagatcggc ttatggcaga ggccgagtgg gcaggggtgg ctttcgacat cgtagacacg
      961 ggaggcattg acccctccct catggcttcc gggaggaaga aagagccttt atcgattgga
     1021 tcggcggatt acattgggca aatccgtgcc caggctgaga tggctatcca ggaggctgac
     1081 gctgtgcttt tcgttacgga tgcagaagat ggggtgacgc ctgcagatcg tgaggtagcc
     1141 caaatcttgc gccgccatca gcgagaggtt gatggagaat cttggccccc ggtatttttg
     1201 gtagttaata aagcggatag tgaagcccgc cgagcgcaag cgctccagtt ctacgaactt
     1261 ggcatgggtg atccccaccc gatctcggcg ttgcatggca ccggtacagg agatttgctg
     1321 gatgtcctgg tcgcctcgtt tcgggagccg ggcgaagagg ctgaagatga cacggtgaaa
     1381 atcgccattg ttggcaaacc aaacgtgggc aaatctagtt tattgaaccg gttggtggga
     1441 caagagcgtg ccattgtcag cccgatccca ggtaccacgc gggatgccgt agacgagcag
     1501 atcatggtgg gtgagatacc cgttaccctg attgatacgg ctggcatccg cagacgggga
     1561 cgcatcgagc cgggcgtgga aaaatatagc gtgttgcgct ctctgcgcgc cattgagcgc
     1621 gccgatgtca gcctgctggt agtggatgca acatcaggta tgacagctca ggacactcat
     1681 attgctggtt tcatcctgga tgcatggaag agcgctgtgg tgctggtgaa caagtgggat
     1741 gccatcccca aagatagcta caccatggac cacttcactc gccaggtacg ccaggagtta
     1801 aactttatgg attacgtccc ggtgttgttc atctcagcta aaacaggcca acgtgtcgaa
     1861 caggtgttgc cactggcatt gcgcgtgcaa gaggagcgct tagtgcacat ctcgacatcg
     1921 gatattaatc gtattattca agatgcacaa aggcggcatg ctgcgccttc gcacgcaggg
     1981 cgacaactca agatgtacta tgggactcag gtgcgtagcg acccgcctac attcatgatc
     2041 tatgtaaatg atcccaagct ggcgcacttc acctaccggc gatttttaga gaaccgcgtc
     2101 cgtgaagcgt atgggttttt aggtacaccg ataaggattg tgctgaaacc ccgcaggtaa
     2161 cctgcagccc ttgcaccacc tgcttacccg ccttataatc cgcctgatta actatggata
     2221 ataatataga cagcataact attgaaaccg ccgacgacca ggcagctatt ccacaaacca
     2281 agacgaagat tgtgcgattt ttggtcgata ttgtagaaac tctggtattc tctctgctgc
     2341 tcttcgcggg gattaacgca cttacagccc ggattcgcgt ggatgggatg agtatggagc
     2401 ctacgcttca ctccggggaa tttgtcattg tgaaccgaat tgcatatcga tttgggcagc
     2461 cagcgattgg cgatgttatt gtatttctat tccctagaga tccaacgcag gaatatatca
     2521 agcgtgtcat cggtctgccg ggagatgagg tggtgatctc gaatagtcag gttcaagtaa
     2581 acggtcaagc tttgaacgag ccatatatcg ctgcacctcc gaagtatgaa agtcaatgga
     2641 cagtgccgga aggctcgctt tttgtgcttg gtgacaatcg caataattcg tctgattcgc
     2701 atacttgggg accggtgccg ttagaaaatg tcattggaaa agcagttttt gtctattggc
     2761 ccccagtgga gtggggtgtg gtggaacata tcaccccggt cgctggttta ccatgagtcg
     2821 attgattggt gtgtttaaat ctgaaaatat tgtcagtgat atcttaacca aaaaaaggtt
     2881 ccatttgcat gtaaaatcat ggtaaactcg aaaatagcag gaggaagcac atcatggctg
     2941 gggaagacac gaacgaagcg aataaattcg tggctctgtg tccagagtgc caaacaggcg
     3001 ttttgcgtct gcgatctttg acctatttca cctggttgaa caccgaattg atcaccgtcc
     3061 caaatttccc cgcctgggtt tgtgatgtgt gcgggcgtag ggaatatgac ccgcgcgcca
     3121 ttaagtggct taatacgctg ctcaacccag aggcagggcg ctcacaaaat cgaccgcgca
     3181 gcatgccccc gcgagtcgat cgccctagac cttaatttgc cagttcttag aaccgttgga
     3241 tatgtggtcg cgccagggag agcgcggcca ctgtttttgc atcggggatt tgtccctttt
     3301 ctgccagctc aaacgcttct ccggcagcca tgcgctcgac atggatgaat tcatcatcat
     3361 cacctggcag gggtgctggt tgcaagctgg tggctaggta aatatataca tactcagtgg
     3421 aataacccgg agccataaaa aactcaccga ttttcaatac ttctccggca gccataccga
     3481 tctcttcacg cagctcgcga taggcgcagc tttcgatatc ttcacccctc tccatcgtcc
     3541 cggcaggcaa ttctagcatt tctgtgccag ccgcatgccg gtattggcgt acgaaccaaa
     3601 tattgccctg cgcgtccacc ggcactaaga ccaccgcccc ggggtgttgg acgatatcga
     3661 gctggacaac gcgtccattg ggcagacgca cctcgtcctg gtgtagatca aatactcgtc
     3721 cacgataaat tcttgatgag ctaatttttt gaaatgtcat aatgttaagc aattggggtt
     3781 tcgggcgggc tgtgaatgcc tgccagaaac ccctggtttt taggatgtcc tgattctaac
     3841 tatctctctg cttatgggat gcagagctgt tgaccgttcg tgagcttgta gggtgcgctc
     3901 aacccgtttg ctaaggcaat agcatatggg tctgcatcgc catactcgca ggcgatggaa
     3961 tagattgaat ccctcgatcc caccgtgtag gtatcgggat gggacttcaa ggagcgtttg
     4021 cccgggaagc ctcgcgcact tttaggaatg gtaagtttcg tgcccaccgc gtaggaacta
     4081 cctgaattca atccgcttgc gtttagcaag tcaaccggat tgacatcgaa gcggcgggca
     4141 atacaatacg gatgctcacc acgctgcagt gtccaggagg atgattggcc tggtgtcgca
     4201 gtgggaatta caatcgaact acaagaagat gatgaagcag cttggggagc agtggttgct
     4261 gtggctgtgg gagcagctgc cgctgccgct tctggggtag acgttgcggc aggtgcaact
     4321 tcgcctgtgg gcgtctctgg ggcggtgact ccgccagcca gggcaaaggc tgtctgggta
     4381 gcaaagctct ccaattggcc catcacgtcc tcagtgacgc caggtagggg gaacccgcct
     4441 tcgggcaccg ttggtgagcc aacgctttcg ggagcaacag aagctggtcg ttcacatgca
     4501 gatatgaaaa taaccaataa aattgtcacc aggagcagcg gaaagcgttt ggatttcatc
     4561 ttttctctcc ttaactgttt tcacccactt ggcctgattg caggcaacca tgcgtgagta
     4621 ttttgttcga aaaccgcagc ggtctctcga actttgcaat gatactagca caatcatggt
     4681 aaagcgtcaa gattcttgca taaatacttg cgaaactgtc tttaagcgac atattgatat
     4741 tttttatgtc ccatccatcg tttgccctca ccatgctcta cccaaagaga cccagggcaa
     4801 tcgcatatga cctgaggggc atgcgcaatc ccgcaaagag agtgtttggg gtgttttggt
     4861 gggggtatac tcccaccaaa acaccccata ttctcttttc agtggtggga aatcgtccta
     4921 atgcgattgc cctacaaaga gaccaaaatt atttgcatta ctagcttgat cgtgttaaac
     4981 taaacgattg ccatgcaacg catctgccta tctcttgtta ttatcattat tatgtatttg
     5041 acgtccactg caaatgttgc ccgagcccaa caggagacgc ccaccccatc cccatcggag
     5101 gtaccgactg ggactcccat ctcgccgcgc cctctgggaa ctcccgagct gtccatacgt
     5161 tctcccctgc ctgggcaggc agtacaaggc agcgtaccaa tcgccggaag cagcgcggtt
     5221 acaggttttg tatctgccga agtatctttt acctatgcag gtgaccagac gggtacctgg
     5281 ttctttattg gcgacaccag cgatgcggtg gtggatggga cgttggcgca gtgggatacc
     5341 agctctatca ccgatggcga ttacgacctg cgtttaagag tcctgcgtga agacgggagc
     5401 tatcttgatg cagtcatctt gggcttgcgc gtgcgcaact atacgccaat cgagacggct
     5461 acaccaacac ccgtcacgcc cactgccaca cagatgcccg gcgagctgac cgcgacatct
     5521 acggccatac ccatgagttt gccctctgtt acgccgctgc caaccaaccc tgctgaaatc
     5581 acaggccagg atattgcctg gggtttgggt aaaggtgcgc tggctgtgat tggcttgttc
     5641 gccttgatgg gcgtttacaa gctggtaaat gagattggga acaaagaaca gtaattattg
     5701 aatggcctta tcgtatgact agtcagcgct ctttagacat gaatcaacct gcaaccttcc
     5761 tgatcattgg cttgggcaac ccaggtcgcg actaccaagg caaccgccac aatattggct
     5821 tcaggctgct gaaccatttg gcggagcggt tgggtgtggg attctctcgg ctcgaatcaa
     5881 aatcgctggt gacgaaggct gattaccttg gtcatcgatt gatactggcc aagccgcaga
     5941 cgtatatgaa cctatctggg caggcagttg aatcgctagt gcgtttttat aaaatcccgc
     6001 tggggcaagt gatggtggta tttgacgatg ttgacctgcc atttggagcg ttacgcttgc
     6061 gccctgcagg aggatcaggc gggcaaaaag ggatgcaatc aatcctcgac aggttgggta
     6121 cgcaggaaat ccctcgactg cgattgggaa ttgacaggcc accgggtcgc atggaagcgg
     6181 ccgattatgt gctgcaggat ttctcaaaaa atgaagtcga gttgttaccc ggcatattag
     6241 accgggctgc tgatgcggtg ctaacctatg ttcgcaatgg gttggatgca gccatgaacc
     6301 agttcaaccc acaaaattcg gatgatcagg gataggtgaa tgtcgatgca acttgatgcc
     6361 atcctcaata ctgtgatgga gactttagga caaaggacat cctatcttct agggcaggtg
     6421 ctgccaagtt ttgatctgcg ccgagcggcg cgcttaccag tcttggtatc catgtacagg
     6481 atgaccaacc agcctgtctt gttgatcacc gaccgggcgg atcacgctct gacattgttt
     6541 gatgaactta gcctgtgggc gccaaacgca aagcgcttgt taattcctga gccgacttcg
     6601 ctcttctacg agaaagcgcc ttggggcgag aacacccgtc gcgagcggct ggtggctttg
     6661 accaccttgg cagcatacca tgtccctggc actacccctt caggaaatga tagcctgaat
     6721 cacgccatcc ttgtggcgcc agcccgtgcc ttgatggcgc gtacgcttcc cctgcgcgag
     6781 ttcctcaaaa gcacgcgaag ccttcgccca ggacagaatg cc
//
LOCUS       DBNGSX010000404         3080 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1144190, whole genome shotgun sequence.
ACCESSION   DBNGSX010000404 DBNGSX010000000
VERSION     DBNGSX010000404.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1144190"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1336)
                     /locus_tag="AC16CB_12030"
     CDS             complement(<1..1336)
                     /locus_tag="AC16CB_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HIR3343244.1"
                     /translation="MAPEETTPPSDFIREIVREDNRTGKFGGRVHTRFPPEPNGYLHI
                     GHAKSIVLNFGIAEEFGGKCNLRFDDTNPTKEEVEYVESIIQDVRWLGYDWEDRLFYA
                     SDYFAQLYEWAVKLIKKGEAYVCDLTADEVREYRGTLTQPGRESPYRDRSVEENLDLF
                     ARMRAGEFPDGARTLRAKIDMSSGNLNMRDPVLYRILHESHHRTGDVWCIYPMYDFAH
                     GQCDSIEDITHSICTLEFQDHRPLYDWFLDELEVYHPQQIEFARLNLSYTLLSKRKLL
                     RLVKDGHVNGWDDPRMPTLSGLRRRGYTPQAIRDFCELIGVAKNDSIVDIALLEHCQR
                     QDLNKHAPRVMGVLRPLKVVIDNYPEGQEEEMEAVNNPEDPSMGTRKVPFSRVLYIEQ
                     DDFREQPPPKFFRLAPGREVRLRYAYFIRCEYAVKDENGEVVELHCTYDPATR"
     gene            1701..1771
                     /locus_tag="AC16CB_12035"
     tRNA            1701..1771
                     /locus_tag="AC16CB_12035"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1733..1735,aa:Gln,seq:ctg)
     gene            1868..>3080
                     /locus_tag="AC16CB_12040"
     CDS             1868..>3080
                     /locus_tag="AC16CB_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR3343245.1"
                     /translation="MTTQFEVHDPTILSQARGVLDACKTSQEMYQAVLDAVKRNEQWR
                     GQHCINLLAPEAPTSPTVRALLSSEVGIRAAEGHIGALNRWFVGTHYIDEIEALCIEL
                     LKQVFKVRYADHRLMGSMLGNLTVYTALTQPGDVIMSIPQPVGGHSSNRLDGPAGVRG
                     LKIVDIPFDPVELEVDLDMFRKMAPLIQPRVVSLGASMTLFPFPVKAMSEVVADWGGH
                     VFFDGAHQLGLIAAGLFQDPIREGAAVLTGSAGKTFSGPQSGVIVWDDPELTGPVTHA
                     IFPVLVATHQVNRVAALAASAAEMLAFGQQYMTQIVRNGKALGAALHKRGIPVLGAHK
                     GFTETQQVIADVGNFGGGLVVAQRLARANLITNKNLIPTDTPANWDRPSGLRMGTIEV
                     TRLGMREAEMEQ"
BASE COUNT          679 a          871 c          825 g          705 t
ORIGIN      
        1 cgcgcgtggc tggatcgtaa gtgcagtgca gctcgaccac ttccccattc tcatctttta
       61 cagcgtactc gcaacggatg aaataagcgt accgcagccg tacctcgcgt cctggcgcca
      121 ggcggaagaa tttgggcgga ggctgctcac gaaaatcatc ctgctcgatg tagagcacac
      181 gggagaaggg caccttgcgc gtccccatgg aaggatcctc ggggttgtta actgcctcca
      241 tctcctcttc ttggccttca gggtaattat cgatcaccac cttgagcggg cgcagcacgc
      301 ccattacacg tggagcgtgc ttgttcaaat cctggcgttg gcagtgctcc agcaaagcga
      361 tatccacgat gctatcgttt ttagctacac cgattagctc gcaaaaatcg cggatcgcct
      421 gcggcgtata gccgcggcgg cgcagacccg agagggtcgg catgcgcggg tcatcccagc
      481 cgttgacgtg cccatcctta accaaacgca gcagcttgcg cttggagagc aatgtatagc
      541 tcaggttaag ccgggcaaac tcgatctgct gtggatggta aacttccagc tcatccagga
      601 accagtcgta cagagggcga tggtcctgaa attctaacgt gcagatggaa tgggtgatat
      661 cctcgatgga gtcgcactga ccgtgggcaa agtcgtacat cgggtaaatg caccatacgt
      721 cgccagtacg gtggtgagat tcgtgcaaga tgcggtacag cactgggtcg cgcatattca
      781 gattaccgga agacatgtcg atcttggctc gcaaggtgcg cgccccatca gggaattcac
      841 cggcgcgcat gcgggcgaac aagtccaggt tttcctcaac cgagcggtct cggtaagggc
      901 tctctctgcc cggctgggtt aacgtaccgc gatactcgcg cacctcgtcc gcagtgagat
      961 cgcacacata agcttcaccc ttcttaatca gcttgaccgc ccattcatag agctgtgcaa
     1021 agtaatctga ggcatagaag agtcgatctt cccaatcgta tcccagccag cgcacgtcct
     1081 gaatgatcga ctcgacatat tcgacctctt ccttggtagg gtttgtatcg tcaaagcgca
     1141 ggttgcattt gccgccaaat tcctcagcta ttccaaaatt aagcacgatc gatttggcat
     1201 gaccaatgtg caggtagccg tttggctcag gtggaaaacg agtgtgcacg cgaccaccaa
     1261 acttgcctgt gcggttatct tcccggacaa tttcacgaat aaagtcgctg ggaggggttg
     1321 tctcttcggg tgccatagca gcgcctctct tatgacctat ttttggatgg taacttaatg
     1381 cacaaattat aacaaaccta tggcgttaat acccctctca ttgatctcct cctgccctaa
     1441 tcccgctgct ttgcccttga acagcctaac acaaatttgt taagcttatg aaaggcttcc
     1501 cctcagaagt gcaagggtgt aactgagaat agcctgctat acttgccaag ccaacatctt
     1561 tctactgtcg agaaagggtt ggatttggga gccgggcgca ggcctggctc ccttatttat
     1621 tacacccatt ctaacgatct cggttcaccc cacctttgac gagccccccc ttacatggtc
     1681 caatctccat cgtcgaacgc tgggggttcg tctagtggta ggacagcaga ctctgaatct
     1741 gtatgccgag gttcgaatcc tcgaccccca gcctgacacc tttttatcgc tccagcacta
     1801 aagcacccca tgggatatcc ttggggtgtt atctatcatc caatgagcta tcgaaaggaa
     1861 tacctcgatg accacacaat ttgaggtgca tgatcccacc attctatccc aggcgcgagg
     1921 cgttttagat gcctgcaaaa catcgcagga gatgtaccag gcggttctgg atgctgtcaa
     1981 gcgcaacgag caatggcgcg gtcagcattg catcaacctg ctggcccctg aagcccccac
     2041 tagccctacc gtacgcgcgc tgctttccag tgaagtgggc atccgcgcgg ccgaggggca
     2101 catcggcgcc ctaaatcgct ggttcgtagg cacgcattat atcgatgaga tcgaagccct
     2161 gtgcattgag cttttaaaac aagtattcaa ggttcgctac gcagatcatc gcttgatggg
     2221 cagtatgctg ggtaacctga ctgtctatac ggccttgacg caacccggtg acgtgattat
     2281 gagcatcccc cagcctgttg gcgggcattc cagcaaccgc ttggatggtc cagccggagt
     2341 gcgcggcctg aaaatcgtag atatcccctt cgatccagtg gaattggagg tcgatctgga
     2401 catgttccgc aagatggctc ccttaataca gcctagagtg gtatctctgg gcgcctccat
     2461 gacgctattc ccattcccgg tcaaagcgat gagtgaagta gttgccgatt ggggagggca
     2521 tgttttcttt gatggagccc accagcttgg cttaatcgcc gccggcttat tccaggatcc
     2581 catccgcgag ggtgctgctg tgctcactgg ctcggcaggc aaaaccttta gcggcccgca
     2641 aagtggtgta atcgtttggg acgacccaga gctcaccggg cccgtcacgc atgccatctt
     2701 cccggtgctg gttgccactc accaggtgaa ccgcgtagca gcactggcgg catctgccgc
     2761 agagatgctg gctttcggcc aacagtacat gacccagatc gtgcgtaacg gcaaagccct
     2821 gggagcagcg ctgcacaagc gcggcattcc cgttttgggc gcgcacaagg gttttaccga
     2881 gacccagcag gtgatcgccg atgtgggtaa tttcggcggc gggctggtag ttgcccagcg
     2941 attggcgcgt gccaacctga tcaccaataa gaatttgatc cccacggata cccctgccaa
     3001 ttgggatcgc cccagcggtc tgcgcatggg cactattgaa gtcacccgct tggggatgcg
     3061 cgaagctgag atggaacaga
//
LOCUS       DBNGSX010000405         3687 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_660541, whole genome shotgun sequence.
ACCESSION   DBNGSX010000405 DBNGSX010000000
VERSION     DBNGSX010000405.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_660541"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="AC16CB_12045"
     CDS             complement(<1..457)
                     /locus_tag="AC16CB_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008335175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3343246.1"
                     /translation="MSKKIYKLLAILMLAALVLSACAPKPTEAPPEPVQETEAPPPTA
                     PPPPPEAAFKACQVTDVGGIDDKSFNATAWKGVQDAMAELGIEGKYLESQQQTDYEKN
                     IKAFIDEGCDIIVTVGFLLGDATAAAAQADPDQKFSIVDFAYDPTIPNVL"
     gene            complement(773..1885)
                     /locus_tag="AC16CB_12050"
     CDS             complement(773..1885)
                     /locus_tag="AC16CB_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 periplasmic-binding domain-containing
                     protein"
                     /protein_id="HIR3343247.1"
                     /translation="MKLDEQSPGRLILVSLLLRRYFLNLFILLAILVLPACGEASPAD
                     PTPTVVALTVTSPPTETVTPSPPATSEASKAVLLAPAGSNPGLSDALQSVLPRLAEQA
                     GLGYQVVSSLSAGELENVRVVVAIPPEDNLNSLVTAAPQTQFLALDMPGAQPAPNLTT
                     VDTRAGRSDQLGFLAGYLAVAITDDWRVGVLSEAGTTGGKSASLGFRNGMTFFCGLCL
                     PFYPPYPNTGYPIAVNLPAGAGPQDWQAAVASLETWQVETVFVYPAVADDQLLADLAA
                     ADINIIIAGPPSDGWRDHWVASLGTSDILQVVPDLWAKILAGQSGEQVALPLGFTGIN
                     PDLLSPGRQHMVDEMLADLLAGYIDTGVDPQTGESR"
     gene            complement(1872..2672)
                     /locus_tag="AC16CB_12055"
     CDS             complement(1872..2672)
                     /locus_tag="AC16CB_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR3343248.1"
                     /translation="MQPKKTPQPKKIYVPTAGNELLEKALENINNNVEVNTLWRVINV
                     NAIDRMGMSDHGPVHVQIIANIALRLTRILLKHDIEMSITKYFGLSDQYAELVVLLAS
                     LLHDLGMSISREGHEEFSLFLANDILHKIMDFLPVEDRTIVTSEVLHAIIAHRSGGKP
                     NTIEAGIVRVADALDMSEGRSRIPYEAGNVDIYSVSAAAIDKVEIAEGEDKPIQINIL
                     MNNSAGLFQVDELLKKKLIGSGIEEYVTVRALIERRREKKLLKEFEIR"
     gene            2907..>3687
                     /locus_tag="AC16CB_12060"
     CDS             2907..>3687
                     /locus_tag="AC16CB_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3343249.1"
                     /translation="MKYHIWTEGCQMNVADSMRVASALEHLGYQSVNRADEADVIVLN
                     TCVVRQSAEDKAYGRLHSLRPLKQQHPELVINLMGCMVGVKGYDHLKKRFSFVDVFSP
                     PSDPGPLVAYLTQDESRALEQAETERRFALMDAQPGDDGSLILPLHERNRLVAAHVPV
                     VLGCSHACSFCIIPYRRGVERSRHVGEIVNEVRSLVDQGVKEVTLLGQIVDRYGKDIP
                     DGPDLASLLRITHQVEGLERIRFLTSHPNWMTDELLDTVAEL"
BASE COUNT          865 a          969 c          972 g          881 t
ORIGIN      
        1 ccaacacgtt cgggatggtc gggtcgtagg caaagtccac gatcgagaac ttctggtccg
       61 ggtcagcctg ggcagctgcc gcagtggcgt cacccagcaa gaagcccacc gtcacgatga
      121 tatcgcaacc ctcgtcgata aaggccttga tattcttctc gtagtcggtc tgctgctgcg
      181 attcgaggta tttgccctcg atgcctaatt ctgccatggc atcctgtacg cctttccagg
      241 ctgtggcgtt gaaggatttg tcgtcgatgc cgcccacgtc ggtcacctgg caagccttga
      301 atgcggcctc aggtggagga gggggtgcag ttgggggagg tgcctctgtc tcctgcacgg
      361 gttccggcgg agcttccgtc ggcttcgggg cgcaggcgct caataccaga gcagccagca
      421 tgagaatagc taacaattta tatatttttt tagacatgcg attatctcct ccttaaagag
      481 atatggaaac tttataatgt gaaatgggta ttgggggaag gaagtgagaa gtcttttcgt
      541 ccagcttacc tccttattct aaatcaagat aaatcgataa agatcgcgcc agttaggttg
      601 cccatgagaa ttcgagcatt aaggatgagc tctacccggc aacaagtttg tacaagtcta
      661 gcacgaagct tgtcaaaatg caacccgtca ttgctcatat tttatgagac tttcacagtg
      721 tcgaaagccc tgatatagac aaagtcctgg tttacatttt gcatgcctgg ggctaacggc
      781 tctccccagt ttgggggtcc acgccagtat cgatatagcc tgccagcagg tccgccagca
      841 tctcatcgac catgtgctgg cggccagggc tgagcaaatc cgggtttatg ccagtaaaac
      901 ctaatggcag tgcgacttgc tcgccactct gacctgccag aatctttgcc cataagtcag
      961 ggacaacttg aagtatgtct gatgtgccaa gagatgccac ccagtggtcg cgccagccat
     1021 ctgatggggg gccggcaatg ataatattga tgtcagcagc agccaaatcc gccagaagct
     1081 ggtcgtcagc taccgctgga tagacgaaga ccgtctccac ctgccaggtt tccagggatg
     1141 caaccgccgc ctgccaatcc tgcgggccag cgccagcagg caagttgacg gcaattggat
     1201 aacccgtatt tggatatgga gggtagaacg gaaggcaaag cccgcagaaa aaggtcatgc
     1261 cgttgcggaa acccaggctg gctgatttac ccccggttgt ccccgcttcg ctcaggaccc
     1321 ctacccgcca gtcgtccgtg atggcaacag ccaggtagcc agccaggaaa cctaactggt
     1381 ctgagcgacc tgcccgagta tcaaccgtcg tcaagttggg ggctggctga gcgcctggca
     1441 tatccaaggc gagaaactgc gtctgtggtg cagccgtaac taaggagtta aggttgtcct
     1501 cgggtggtat ggcgacaaca acacgcacat tttctaactc gcccgcgctt aatgaagata
     1561 caacctgata acccaatccg gcttgctccg ccagacgggg caaaaccgat tgcagcgcat
     1621 cagaaagccc cggattggag cccgcagggg cgagcaaaac tgcctttgac gcttcgctgg
     1681 ttgccggagg cgagggtgtg acagtctcag tgggaggcga cgtaaccgtc agcgccacga
     1741 cggtcggcgt tgggtcggcg ggtgaagcct ccccgcaggc aggcagaaca agaattgcta
     1801 agagtataaa taaatttagg aaatatcgac gcaatagtaa gctcaccagg attaaccttc
     1861 caggggattg ttcatctaat ttcaaattcc ttcagtaact tcttttctct tcttctctcg
     1921 atcaaggctc tcactgttac gtattcttca attcccgacc cgatcagctt cttcttgaga
     1981 agctcatcca cttgaaaaag accggcagaa ttgttcatca ggatattaat ttggatgggt
     2041 ttatcctctc cctcagcaat ctccaccttg tcgatggctg ccgcagagac cgaataaata
     2101 tccacgtttc ccgcttcata tgggattctg gaacgacctt cgctcatatc cagcgcgtcc
     2161 gcgaccctta caatcccggc ctctatcgtg ttgggtttgc cgccgctgcg gtgagcgatg
     2221 atggcatgca ggacttccga agtgacaatc gttctgtcct caaccggtaa aaaatccata
     2281 atcttatgaa gaatatcatt tgccaggaat aggctgaatt cctcatggcc ttcgcggctg
     2341 atcgacatgc ctaaatcgtg caaaaggctt gccaaaagga cgacaagctc agcatattgg
     2401 tctgagaggc caaaatattt cgtaatcgac atttcaatat catgtttcaa caggatgcgc
     2461 gtcagcctca gggcgatatt ggcaataatc tgaacgtgga cagggccatg gtcgctcatc
     2521 cccatccggt caatggcatt gacattgatc actctccaga gggtatttac ctcgacgttg
     2581 ttattgatat tctccaatgc cttttcaagc aactcatttc cggcggtggg aacatatatc
     2641 tttttgggtt ggggggtctt tttgggttgc atagtaaata taaaatgata tacggatttt
     2701 tctgacttat tgagtatcat tataacttga tactgtctcc tgccttacat tcattaacag
     2761 ggcaatcgca tttgggcgat tgcccaccac gaggagagaa aatggggtgt tttggtgggg
     2821 gtatgcccct cagggcatat gcaattgccc tgcattcatt aatcgcaatt taccattccc
     2881 ctatctcttt tgttataata acggacatga aatatcacat ttggaccgag ggctgccaga
     2941 tgaacgtggc cgattcgatg cgcgtggcat cagcgctcga acacctaggc taccagtctg
     3001 tgaatcgcgc cgatgaagcg gacgtgatcg tgctgaatac ttgcgtggtt aggcagagcg
     3061 ccgaagataa agcctacggg cgtctgcatt cattacgccc gctcaaacag caacacccag
     3121 aactagtgat caacttgatg ggatgtatgg tgggggtaaa aggatatgat catctaaaga
     3181 aacgtttttc gtttgtagac gtgttctctc caccatccga tcctggcccg cttgtggcct
     3241 acctgacaca agatgagagc cgcgcgctgg aacaagccga gactgaacgg cgtttcgccc
     3301 tgatggacgc gcaacccggc gacgacggca gcctgattct tccccttcac gagcgcaacc
     3361 ggctggtggc agcgcatgtc ccggtggtat tgggttgctc gcatgcctgc tcgttttgca
     3421 tcatccccta tcgtcgcggg gtggaacgca gccgccatgt gggcgagatt gtcaatgaag
     3481 tgcgctccct ggtcgatcaa ggggtcaaag aggtcaccct gcttgggcag attgttgacc
     3541 gctacggcaa agacatcccg gacgggccag acctggcctc cctactgcgc atcacccacc
     3601 aagtggaagg tctggagcgc atccgcttct tgacatctca ccccaactgg atgactgacg
     3661 agctgctgga cacggtagcg gagctgc
//
LOCUS       DBNGSX010000406         1715 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1007461, whole genome shotgun sequence.
ACCESSION   DBNGSX010000406 DBNGSX010000000
VERSION     DBNGSX010000406.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1007461"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="AC16CB_12065"
     CDS             complement(<1..328)
                     /locus_tag="AC16CB_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343250.1"
                     /translation="MSRLLLSLIVATHMLLSATVGCSRIERLPQAAHEAVIDQAKADY
                     AGWLAPPVSFDQLQEISIRRAWRAKNPPTEYSLTDDNPTMWCVELVMSGQQMGSRVEA
                     SEIWVAT"
     gene            complement(360..1472)
                     /locus_tag="AC16CB_12070"
     CDS             complement(360..1472)
                     /locus_tag="AC16CB_12070"
                     /inference="COORDINATES: protein motif:HMM:NF021507.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HIR3343251.1"
                     /translation="MKRVLLLVFSIILLGAATLASVGAGASLTSQTLPSGLAIPTGFE
                     KVTAVYGAQLYRKDYQGGNPDFVQVVDLSLGAKIELLHGSIKEKREGHGAYGGDDPRI
                     LSRSLQQYWDEFTANHANPFCVVNGQFFYMPESPTRLPFPLKVDGQIVSDGFGKQQFP
                     GQKLMLELWRGRADIRQLSKAALYSSTAPNIVAGLAEDARKAINKYVGRTFIGVVDKD
                     GDARYETVLVFTTRSARQVDAAEVLRSFGAQKVMMLDGGGSTQMICMDKSYITSERLI
                     PQALGVQGGVPPSPSNKKAQISSAETSSSAEAEDSPQAKPVQVASEPSEPAQVGASQI
                     ALAQPLYDLGNILWVPGMMLPAMAFILLLVLRTRFN"
BASE COUNT          399 a          450 c          469 g          397 t
ORIGIN      
        1 ttgtggcaac ccaaatctcg cttgcctcta ccctggagcc catctgctgc ccagacatca
       61 ccagctccac gcaccacatg gtagggttat catctgtaag ggaatattct gtaggtgggt
      121 tcttggcgcg ccacgcgcgg cggatactga tctcttggag ctggtcaaag cttactggtg
      181 gggcaagcca ccctgcatag tctgctttag cctggtcaat aactgcctcg tgagctgctt
      241 gtggaaggcg ctcgatcctt gagcaaccaa ccgttgcact cagcagcatg tgggtcgcga
      301 ctatcaagga taataataat cgcgacatgt ctactttttc cactcttctc ggcttgtggc
      361 taattgaatc tcgtcctgag cacaagcagg aggatgaacg ccatggcagg gagcatcatg
      421 cctggaaccc ataagatatt ccccaagtcg tataatggct gggcgagcgc gatttgggat
      481 gcgccaacct gggcaggctc actcggctct gatgcgacct gtactggctt ggcttgtggt
      541 gaatcttcag cctcagctga gctagacgtt tcagcggaag agatttgcgc cttcttgttg
      601 gacggagatg gcggcacgcc cccctgcacg cccagggctt gggggatcag gcgctctgag
      661 gtgatgtacg atttgtccat gcagatcatc tgggtggagc cgcccccgtc aagcatcata
      721 accttctgcg ccccaaagct gcgcagcacc tcggccgcgt ctacctggcg ggcggagcgg
      781 gtggtgaaga ccaaaacagt ttcgtagcgg gcatcgccat ctttgtcgac cacgccgata
      841 aaggttctcc cgacatactt gttgatcgct ttgcgagcat cctcagctag gccagcgaca
      901 atgtttgggg cagtcgagct atacagagct gctttagaga gctggcggat atcggcccga
      961 ccacgccaaa gctccagcat cagcttttga ccaggaaatt gctgctttcc aaagccatcg
     1021 ctaacgatct gtccgtccac cttgagaggg aacggcaaac gcgtagggct ttctggcatg
     1081 tagaagaact gcccattgac cacgcaaaac gggttggcgt ggtttgcggt aaattcatcc
     1141 cagtattgct gcagtgaacg ggaaagaatg cgcgggtcgt cacccccata agcgccgtgt
     1201 ccctcccgct tctctttgat tgagccgtga agtagctcga ttttcgcccc cagggatagg
     1261 tcaaccacct ggacgaagtc ggggttaccg ccctggtaat ccttacggta gagctgggcg
     1321 ccatagacgg cggtgacctt ctcaaaaccc gtggggatag ctagaccgct gggaagggtt
     1381 tgagaggtta gcgaagctcc cgccccaacg gatgctaaag tcgctgcgcc gagcaggata
     1441 atcgagaaga caagcaagag tacgcgtttc attgatggtt gcaaacattg ggtgcaaatg
     1501 atgatgcgtg ggatgtaaaa tattgcaccg gcaaaattat tttagcaaat attttcaata
     1561 aattgtagca cgtacgttct aatttgagaa ggaaaaatat ctggtttcta cgtgcctatc
     1621 gaaaggcgcg cagaaaccag agtatcgaaa atatcttcaa tcgctcataa tgatgagttc
     1681 tgaaggataa attatatccc cagcaatgcc ccacc
//
LOCUS       DBNGSX010000407         2660 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_244740, whole genome shotgun sequence.
ACCESSION   DBNGSX010000407 DBNGSX010000000
VERSION     DBNGSX010000407.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_244740"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="AC16CB_12075"
     CDS             <1..708
                     /locus_tag="AC16CB_12075"
                     /inference="COORDINATES: protein motif:HMM:NF051685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7916 family protein"
                     /protein_id="HIR3343252.1"
                     /translation="GIPMKDAPGGIKIFGRIPSGQGATIGVVKSFVGRPVGLNLEPIE
                     NPEAVTGSGRLANADNARIAVEQGADFILVTGNPHTGVTNRGIRRALAEIRAAVGDRV
                     VLLAGKMHAAGVSGPPVSDADISAFADEGADGIVLPAPATVPGITVEIGHRLAEIAHK
                     RGLLVMNTIGTSQEGASRSTIEQLALWSKMTGADIHHIGDAGTIGIAVPENIYTWSLA
                     LRGRRHTWHRMAASLRR"
     gene            713..>2660
                     /gene="ptsP"
                     /locus_tag="AC16CB_12080"
     CDS             713..>2660
                     /gene="ptsP"
                     /locus_tag="AC16CB_12080"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIR3343253.1"
                     /translation="MQSLRLNILNETGLHARPAATFVKTAKKFQASVRVRNITSGSEW
                     ADAKSVLGVLALGVEYGHEIELEIQGDDEDLAAEALQEAIRSGLGEGIVPPQPVQNEL
                     PQTLRGIPAAPGIAQGTAVLWREATLHIPRFTQTDPAAELERIRIAKQSAQEEIIQLR
                     EKVATEASAEEAAIFEAHLLIADDSSLLGQIETSVNQGVNAEAAWADGVDYFAGQLEK
                     LGDPILQARAMDVRDVGQRVLRHLLGETREAGLDLRQPSLVVARDLTPSQTAGLPKDL
                     VLGFCTAEGGPTSHTAILAKALGLPAVVGLGVDLLDVPDGTPLLVDGTRGEVIVRPDR
                     AQLQAFKALQASRTEQALAEQAVAAEAAITLDGHAVEVVANVGEVEDALRSLEVGAEG
                     IGLLRTEFLYLRRESAPTEEEQVQAYTSILEVMGKRPVVMRTLDVGGDKMLPYLDLGR
                     EANPFLGWRAIRLCLDQPDLFKAQLRAMLRAGVGHDLRIMFPMIATLDELRQAKVLLE
                     QARGEVQSAGQAAATSLQVGMMVEVPAAAMMADIFAREVDFFSIGTNDLTQYTMAAER
                     TNQKVAYLGDGCHPAVLRQIQQVVIAGHANGAWVGVCGELAGDPEAIPILVGLGVDEL
                     SMAPAAIPRAKAIIRRWRLQDARQL"
BASE COUNT          540 a          707 c          876 g          537 t
ORIGIN      
        1 ggtattccca tgaaggatgc ccctggagga atcaagattt ttggtcgcat tccctcgggc
       61 cagggcgcta caataggtgt ggtcaaatca ttcgttggac gcccagttgg cttgaacctg
      121 gaacccatcg agaacccaga ggccgtgacg ggctcggggc ggctggctaa cgcggacaac
      181 gcccgcatag ctgtggagca aggcgcagac ttcatcctgg tcaccggcaa tccccacacc
      241 ggcgtgacaa accgcggcat tcgccgcgcc ctggcagaaa tacgcgccgc ggtgggtgat
      301 cgggttgtgc tcctggcagg gaaaatgcat gctgcggggg tgtctgggcc acccgtaagt
      361 gatgcggata tcagcgcctt cgccgacgaa ggagcggatg gaatcgtctt gcctgctccg
      421 gctactgtgc ctggcatcac ggtggagatt gggcatcgtt tagctgaaat cgcccataaa
      481 cgtggcttgc tggtgatgaa caccattggg acatcccagg aaggagctag ccgctcgact
      541 attgagcaac tggcgctgtg gtcgaaaatg acgggtgcag atatccacca tattggcgac
      601 gcaggcacaa taggcatcgc tgttccggag aatatctaca cctggtcctt ggctctacgg
      661 ggacgacgcc acacctggca ccgcatggca gcttctttgc gcagataatc caatgcagtc
      721 tttgcgatta aacatcctca atgagacggg cttgcacgct cgccctgccg ccacctttgt
      781 caagacggcg aaaaaatttc aggccagtgt ccgggtgcgc aacatcacca gcggaagcga
      841 gtgggcggat gctaagagcg tgctgggcgt cttggctctg ggggtagaat acgggcatga
      901 gattgagctg gagatccagg gggacgatga agacctggcg gcagaggcac ttcaagaggc
      961 gattcgatct ggcctgggtg aggggatcgt gcctcctcag ccggtacaga atgaacttcc
     1021 gcaaacattg cgcggtattc ctgctgcccc tggcattgcc cagggtacgg cggttttatg
     1081 gcgtgaggca accctgcaca tccctagatt tacccaaaca gaccccgcgg ccgagttgga
     1141 gagaattcga attgctaagc aatcagcgca ggaagaaatc attcaattaa gagaaaaagt
     1201 tgccacggaa gccagtgcgg aagaggccgc tatcttcgag gcgcatctgc tcatcgccga
     1261 tgatagctcc ctgcttgggc aaattgaaac gtcggttaat caaggggtaa acgccgaggc
     1321 cgcctgggcg gatggggtgg attactttgc cggtcagctg gagaagcttg gagatcccat
     1381 tttgcaggcg cgggccatgg atgtgcgcga tgtgggtcag cgggtcttga gacacctgct
     1441 gggagagacg cgcgaagctg ggctggattt gagacaacct tccctggtcg ttgctcgcga
     1501 tctgaccccg tcgcaaactg ctgggctgcc aaaagacctg gtgctgggtt tctgtactgc
     1561 agagggtggg cctacgtctc atacggctat cctggccaaa gccctgggtc tgccggcggt
     1621 tgttggtctg ggagttgatc tcctggatgt gcccgatggc acccccctgc tggtggatgg
     1681 cacgcgcggc gaggtgattg tgcgccccga tcgcgcccaa ctccaggcgt ttaaagcctt
     1741 gcaggccagt cgcacggaac aagcattggc tgagcaagcc gtggccgcgg aagcggcgat
     1801 taccctggat gggcatgccg tcgaggtggt ggcaaatgtg ggggaagttg aggatgcgct
     1861 gcggtccctt gaggtcggcg cggagggcat cggtctgctg cgcacggaat ttctctatct
     1921 gaggcgggag agcgcaccaa ccgaagaaga gcaggtgcag gcctatacca gtatcctgga
     1981 ggttatgggt aagcgcccgg tggtcatgcg caccttggac gtggggggcg acaagatgct
     2041 gccctatctg gacctgggcc gtgaagccaa cccgttcctc ggctggcgag cgatccgctt
     2101 gtgtttggac cagcccgacc tgttcaaagc ccagctgcgg gcgatgctgc gcgctggcgt
     2161 gggtcatgat ttgcggatca tgtttcccat gattgctaca ttagacgaac tgcgccaggc
     2221 aaaagtgttg ttggagcagg cgcgcggcga agtgcagtct gctggtcagg cagccgcaac
     2281 ctcactccag gtcggtatga tggtggaagt cccggcggcg gccatgatgg cagatatttt
     2341 cgcccgcgaa gtggattttt tcagcattgg caccaacgac ctcacccaat acacgatggc
     2401 agctgagcgc accaatcaaa aggtagcgta tttaggggat ggctgccacc cggcggtgct
     2461 gcgccagatt caacaagtgg tcattgctgg gcacgcaaac ggagcttggg tgggggtttg
     2521 tggagagctg gctggcgacc ctgaggcaat cccgatcttg gtgggattag gcgtcgacga
     2581 gcttagcatg gcgccggctg cgattcctcg ggcgaaggcc attatccggc gctggcgatt
     2641 gcaggacgcc cggcaattgg
//
LOCUS       DBNGSX010000408         3033 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1422482, whole genome shotgun sequence.
ACCESSION   DBNGSX010000408 DBNGSX010000000
VERSION     DBNGSX010000408.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1422482"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="AC16CB_12085"
     CDS             <1..856
                     /locus_tag="AC16CB_12085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3343254.1"
                     /translation="YQRLYEDAVARYQRLQMPPDPNDLAVAEAKLKLAQAQLRQAQSD
                     WERVKDGPNPADIAVLQARLDDSRRTAEHLKDGPDPDEVTAARARLAAAQAALDLAHI
                     KAPFDGVITEAAAQPGDQVLPGDVAFRLDDLSPLWIELDVSEIEVNRIREGQPVIITL
                     DSSPGRQYNGEVVDVSAVGKISSGVASFRVKAEIRDADRASRPTMTASASIIVAEMKD
                     VLIVPASAVRFIDGQRVVYVLRQGQPTPVHVTLGSFSSNEIQVLEGDLQPGEAVILNP
                     DDREQGSS"
     gene            893..2443
                     /locus_tag="AC16CB_12090"
     CDS             893..2443
                     /locus_tag="AC16CB_12090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3343255.1"
                     /translation="MRKWWIGGIAVLILVSAGLAISFLRSQRQAAALSGLLTEPAQRG
                     DLTATVGATGVVRANQTALLTWQTTGTVGEVNVGVGDQVAKEQVLAVLDQTSLPQSII
                     LAQAELVTAQRALEDLLNSQIQQAQALQAVDDARQALEDAGNPELAQAKAQQAIADAQ
                     KAVENAERLLRAAKSPASQSFIDEAQARVVLTRDILEQAQERFEPYQNKPQDNLIRAR
                     LQTELAAAQQQYDAAVRILNSLQGTSNPIDQAVAQADLETTQAQLVEAQREWERVKDG
                     TSPAALALLEAQLADAEREFERVKNGPDPDDIAAAQARVAAAQAAINLASIHAPFAGM
                     ITQSESKTGDQTAPGSVAFRLDDLSRILVDVQISEVDINRIQIGQDVNLVFDAIPNQE
                     YHGTVTEVALVGTSSQDVVDFIVTLELVDADQAVKPGMTAAVNIVVNQLKDVLLVPNR
                     AVRILEGKRVVYLLRGEAPDPVEIQLGASSEASSEVIGGDLKEGDPIVLNPPTVFEQN
                     GPPPFVRR"
     gene            2446..>3033
                     /locus_tag="AC16CB_12095"
     CDS             2446..>3033
                     /locus_tag="AC16CB_12095"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3343256.1"
                     /translation="MDNWVIEAENLTKVYKMGEVEVHALRGLSLRISRGEVAAIMGPS
                     GSGKSTLMNMIGCLDQPTSGEYILDGERVSNLKDDQLATIRNRKVGFVFQSFNLLPRA
                     TALANVELPLRYAGIQRGRKERARAALELVGLADRIHHRPNELSGGQQQRVAIARALV
                     NNPAIILADEPTGNLDSKSGEEIMQLLLGLNQEHGT"
BASE COUNT          728 a          846 c          878 g          581 t
ORIGIN      
        1 gtaccagcgg ttatatgagg atgcagtagc aaggtaccaa cgactccaaa tgccgccgga
       61 ccccaatgac ctggcggtgg cagaagccaa attgaagctc gcccaggcgc agttaagaca
      121 ggctcaaagt gactgggagc gcgtgaaaga tggccccaac ccggcggata tagcggtgct
      181 ccaggccagg ctggacgatt cccgtcgcac agccgagcac ctgaaggatg gccccgatcc
      241 agatgaagtt actgcagcac gagcacggct ggctgcagcc caggccgcgc ttgacctagc
      301 acatatcaaa gctcccttcg acggcgtgat caccgaggct gccgcacagc ctggagacca
      361 ggttctccct ggtgacgtag ctttccggct ggacgacctg tcaccgttat ggatagaatt
      421 ggacgtctcg gagatcgagg tcaaccgtat ccgtgaaggc cagcctgtaa tcattacgct
      481 ggactcatcg ccaggacgcc aatataatgg cgaggtggtc gatgtttctg cagtcggcaa
      541 gatctccagt ggagtggctt catttcgcgt caaggctgaa attcgcgacg ctgaccgcgc
      601 aagccgccca accatgaccg cttccgccag tatcatcgtc gctgagatga aagatgtgct
      661 gatcgtgcca gcaagcgccg tgcgctttat cgatggacag cgggtggtgt acgtgctgcg
      721 gcaagggcag cctaccccag tgcatgtcac gctgggttct ttttcatcta atgagataca
      781 ggtgctggaa ggcgatctgc aaccaggaga agcggttata ttgaatcctg atgaccgaga
      841 acaaggcagc tcgtgatatt cttgagctaa gtaaaatggt gttggaggaa gtatgaggaa
      901 atggtggatc ggcggtatcg ccgttttgat cctagtcagt gcaggtttgg caataagctt
      961 tttacgcagt caacgacagg ctgccgcgtt atctggactg ctaaccgagc cagcccagcg
     1021 cggcgattta actgccacag taggcgctac cggtgtggta cgcgctaatc aaaccgcatt
     1081 attaacctgg cagaccacgg gcacagtggg cgaggtgaat gtcggggtag gcgatcaagt
     1141 cgcaaaggag caggtactgg cagtgctcga tcagacatct ttgccccaat caatcatcct
     1201 ggctcaagct gaactggtta ccgcccagcg cgcgctcgaa gacctactca actcgcaaat
     1261 ccagcaggca caggccctcc aagccgtcga tgacgcccgc caagccttag aggatgcagg
     1321 caatccagaa ttagcacagg caaaagcaca gcaagccata gctgacgcgc agaaagctgt
     1381 cgagaatgcc gaacggttat taagggcagc caaatcgcct gccagccaga gcttcattga
     1441 cgaggcgcag gctcgggtgg ttcttacacg agatatcctg gagcaagcgc aggagcgttt
     1501 tgagccttat cagaataagc cacaggataa tctgatacgt gcgcgcctgc agaccgaatt
     1561 agccgccgcc cagcagcaat acgatgccgc cgtaaggata ttgaacagcc tacagggcac
     1621 gtccaacccc attgaccagg ccgtggcgca agccgactta gagaccaccc aggctcaatt
     1681 agtcgaagct cagcgcgagt gggaacgcgt taaggatggc accagcccag ccgcgctcgc
     1741 tttgctggaa gcacaactgg cagacgccga acgcgaattt gagcgcgtga aaaacggccc
     1801 tgacccagat gacatcgccg ccgctcaggc gcgcgtcgct gccgcacaag ccgccataaa
     1861 tctggcatct atccatgctc cttttgcggg gatgatcaca cagtcagaga gcaagaccgg
     1921 ggatcaaacc gccccaggaa gcgtggcatt ccgcctagat gacctttcca ggatattggt
     1981 ggatgtgcag atctctgaag tggatatcaa ccgcattcaa atagggcaag atgtgaacct
     2041 tgtcttcgac gccatcccaa accaggaata tcacggcaca gtgactgagg ttgctcttgt
     2101 gggtacttcc agccaggatg tggtcgattt tatagtaacc ctggaactgg ttgatgctga
     2161 ccaagcagtt aagccgggca tgaccgccgc ggtcaacatc gtggttaacc agttgaaaga
     2221 tgtgcttctg gtccccaacc gtgcagtacg tattttggag ggaaagaggg tcgtttacct
     2281 gctgcgcggg gaggccccag atccggtaga aatccagctt ggggcatcct cggaggcatc
     2341 gagtgaggtg attggcggcg atttgaagga gggcgaccca atcgtgctca atccaccgac
     2401 agtctttgag caaaacggcc ctccgccatt tgtacggagg taacgatgga caattgggtc
     2461 atcgaagctg agaatctgac caaagtatat aagatgggcg aggtagaggt gcatgccctt
     2521 cgcgggttgt ccctcaggat ttcccgcggt gaagtagccg ccattatggg accatcaggc
     2581 tcgggcaaat ccaccctgat gaacatgatc ggctgcctgg accagcccac ctccggcgag
     2641 tatatcctgg atggggaacg cgtctcgaac ttaaaggacg accagctcgc cactatccgc
     2701 aaccgcaaag tgggctttgt cttccagagc ttcaacctgc tgccacgtgc cacggcatta
     2761 gccaatgtag agctgccctt gcgctatgca ggaatacagc gcgggcgcaa ggaacgcgcc
     2821 cgcgcagctc tagagctggt ggggctggcc gatcgcatcc accaccgtcc caacgagctt
     2881 tcgggtgggc agcaacagcg cgtcgccatc gcccgcgccc tggtaaacaa ccccgccatc
     2941 atcctggcgg atgagcccac cggtaacctc gacagcaagt cgggcgagga gatcatgcaa
     3001 ttgttattgg gacttaacca ggaacatggc acc
//
LOCUS       DBNGSX010000409         5318 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1492108, whole genome shotgun sequence.
ACCESSION   DBNGSX010000409 DBNGSX010000000
VERSION     DBNGSX010000409.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5318)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5318)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5318
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1492108"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /gene="alr"
                     /locus_tag="AC16CB_12100"
     CDS             <1..564
                     /gene="alr"
                     /locus_tag="AC16CB_12100"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR3343257.1"
                     /translation="RAAQGSGLHIPIVHAANSAATLNLPQAHFNAVRPGIALYGLQPS
                     SEWPSPIEIRPALTLKSRVSRVRQLPAGAGVSYGRTFVTQHPTIAALIPVGYGDGYPR
                     ALSNRSSVLIRGRRAPIIGRVCMDQFVVDVSDIPGVQLDDEVVLIGVQGNENIQAEKL
                     AELAGTINYEITTSLLPRIPRLYIRAG"
     gene            complement(597..1259)
                     /locus_tag="AC16CB_12105"
     CDS             complement(597..1259)
                     /locus_tag="AC16CB_12105"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014305.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3343258.1"
                     /translation="MNGDLQTQVIHVGDWVIRQRVPAGEGPFPTIIMLHGWTGDENVM
                     WIYATRMPKNALLLAPRGLYPTPLGGHGWHPYKAKVWPLMDDFRASNEAFLELLNINN
                     FPLVDFSRLHFVGFSQGAAMSYAFALENGDRLSSVVGLSGFLPIGADAAATNESLHHK
                     PIFIAHGNQDFLVPVYRARETVNLFQKAGADVTYCEDDVGHKLSANCFRGMEAFFVKQ
                     GF"
     gene            1533..1877
                     /locus_tag="AC16CB_12110"
     CDS             1533..1877
                     /locus_tag="AC16CB_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343259.1"
                     /translation="MNSATTWRTIFEKEILQAESARAAGNEGMARVCARRAAGVVAGE
                     YLRRQGLSLSNPSAYARLKFLSQIPQTPPTIQEIVDHFLIKIDFNHSLPVRADLIAEA
                     RWLANELLSWPN"
     gene            1894..2223
                     /locus_tag="AC16CB_12115"
     CDS             1894..2223
                     /locus_tag="AC16CB_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007339783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3343260.1"
                     /translation="MSDNYLYVGDLAAQIPELQPDSIISRTIYTDDQIKVILFAFAPG
                     QELSEHTASVPATLHFLEGEAQLILGEDEHEAMPGTWVHMPAKLPHSVYAKTLVKMLL
                     IMHTQKA"
     gene            2224..3300
                     /locus_tag="AC16CB_12120"
     CDS             2224..3300
                     /locus_tag="AC16CB_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343261.1"
                     /translation="MIQGRFRFPFLFLALLGLIAAMWAGWIRLGWDWPPIQPSLPASH
                     GPLMVVGFLGTLIALERAVALRQRWMYFGPLLSGIGGLVTIIGIPGLLGPFLMVLGSL
                     VLVAILIVILRQHIALHTVAMALGATSLAIGTILLFIGWSIPRIVLWWEAFLVLTIAG
                     ERLELGRLLKLSKGIVWAFSAVTALSLLGLATSTISLIWGTRIFGLGLLGMAAWLLRY
                     DIARHTIRQNGLARFAALSMLSGYGWLAIGGLLALIYGGQAAGPVYDALLHSVFIGFV
                     MTMIFAHAPIIFPSILGLPISFSRLFYTHLILLHASLLLRIIGDLAMVMPLRRWGGLL
                     NGIAILTFLVITIVSMLTNRERSI"
     gene            3320..4081
                     /locus_tag="AC16CB_12125"
     CDS             3320..4081
                     /locus_tag="AC16CB_12125"
                     /inference="COORDINATES: protein motif:HMM:NF014632.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF166 domain-containing protein"
                     /protein_id="HIR3343262.1"
                     /translation="MRILAIISGDYGTRHVENIRKFGPPSWTINTWQAPAAFPLIIDY
                     PEDYIPQELPETDLVLSFAEHRGVAELLPDIAKMTNAKAVVASIDNESWLPRGLARQL
                     RGWLADIGVACATPKPLCSLTENDFGVTRRQRIKFESPYIAEFATCFGQPSLIIQVDP
                     QSRIIQKAEVRRDAVCGCARYVAERLVGISADDASEKAGLLHHHYPCLASMGKDSDFG
                     DTLMHVSGNVLKDNVEVQVRPYKRIQYISPGTRSE"
     gene            complement(4113..4421)
                     /locus_tag="AC16CB_12130"
     CDS             complement(4113..4421)
                     /locus_tag="AC16CB_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343263.1"
                     /translation="MYKMARSFLKGNIKDVRDFEARLEATLKPVRPPEEFVRDLREKL
                     LAQIRQAESGSKRSTKQIILIVAAGFLSVILIIVAGLRTLASFIIGLKLIKRVRKTKS
                     "
     gene            complement(4457..5035)
                     /locus_tag="AC16CB_12135"
     CDS             complement(4457..5035)
                     /locus_tag="AC16CB_12135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3343264.1"
                     /translation="MSELSDESILARAANGDGEAFGALYERYVSRIYNYIYYRTGNAH
                     DAEDLTARVFYRAMRHISNYNDRGLPISAWLYRIAHNLVANWHRDNSRRKEIPLEESV
                     HGRFDSSHPEHDLLKFEERDRLIRIIRRLSAERQQLLILKFVEHMSNAEIGQIMGRSE
                     GAVKSLYHRTLLALRDEYELLMTDALTESNYE"
BASE COUNT         1354 a         1356 c         1206 g         1402 t
ORIGIN      
        1 cgcgccgctc aaggaagcgg attacacatt ccaatcgtcc acgccgccaa cagcgcagcg
       61 acattaaacc ttcctcaagc tcacttcaat gccgtgcgcc cagggatcgc tctctatgga
      121 ttacaaccat ccagcgagtg gccgtccccc attgagattc gcccagcctt aaccctaaag
      181 agccgggtta gccgggtacg ccagctaccc gccggggcag gagttagcta tggtcgcact
      241 tttgtcacac aacaccccac aatcgctgca ttaatacctg tcggttacgg ggatggctac
      301 cctcgcgccc tctcaaacag aagcagtgtg cttatacgag gtcgccgcgc acccatcata
      361 ggtcgggttt gcatggacca attcgttgtt gatgtcagtg atatccctgg cgtacaactt
      421 gatgacgagg tggttttaat tggggttcag ggtaatgaaa atattcaggc agagaaatta
      481 gctgagctag caggtacaat caactatgaa attactacca gcctgcttcc gcgcatacca
      541 aggctataca tacgcgctgg ttagttatcc ctacaccatt tccccctccg gaacggctaa
      601 aacccctgtt tcacaaaaaa cgcttccatt ccacggaaac aattggcgct cagcttgtgg
      661 cctacatcat cctcacagta ggtcacatca gcgcctgctt tttgaaataa attgactgtt
      721 tcccgtgccc ggtagactgg taccaggaaa tcctggttgc cgtgggctat aaatataggt
      781 ttgtgatgaa gactttcgtt tgttgcagca gcatcagccc caattggaag aaatcccgag
      841 agtcccacca cagagctcag gcgatcacca ttttccagag cgaaggcata ggacatggct
      901 gccccttggc tgaatccaac aaaatgcaaa cgactgaaat ctaccaaagg gaaattgttg
      961 atgtttagca actctaaaaa agcctcattg gaggcgcgga aatcatccat taaaggccag
     1021 acttttgctt tatagggatg ccatccatgc ccgcctaatg gcgtggggta taagcctctc
     1081 ggtgcaagca agagagcatt tttcggcatt cgggtggcat agatccacat tacattctcg
     1141 tcgccagtcc aaccgtgcaa cataataatg gtggggaaag ggccctcacc agccggcacc
     1201 cgctggcgta tcacccagtc tccaacgtga ataacctgag tttgtaaatc gccgttcatg
     1261 tgtttgctcg tcgcactgcc caatctagaa taaataatat aagcccgatc aagactagcg
     1321 gagataaacc taacccaagg caaaaaatcc ctaaaattgc tgcttctttt ccgtagaata
     1381 tatagatcag tccatcacct acaaggaaca atagcaagat gccacccaaa atcagtcgaa
     1441 tctgtgtctg gcgagcatat ctacgcaaat ctcgaatcat ttagatcgat tataacattt
     1501 tgagtaatcg acttttacgg tacaatttgt ttatgaacag tgcaaccacc tggcgaacga
     1561 tttttgagaa agagattctc caggctgagt ctgcacgtgc ggcaggtaat gaaggtatgg
     1621 cacgagtttg cgcccgccgc gcggctgggg tagttgcagg ggaatatctg cgccgacagg
     1681 gattatcact ttcgaacccc agcgcgtatg cacggctgaa atttctcagc cagatacccc
     1741 agacaccacc cacaattcag gaaattgtcg atcatttctt gatcaaaatt gatttcaatc
     1801 acagcttacc cgttcgcgcc gacctaattg ctgaggcgcg ttggcttgcg aatgaattgc
     1861 tttcatggcc aaattgaata aggacaacat attatgagcg acaactatct ctatgtaggt
     1921 gatctggctg cccagatacc tgaattacag cccgattcca tcataagccg aacaatctat
     1981 accgatgatc agataaaagt aattctattt gcatttgccc caggacagga actctctgaa
     2041 catacagcct ctgtcccagc gacattacac tttctagaag gggaagctca actaattctt
     2101 ggtgaagacg agcatgaagc catgccagga acctgggtac acatgcctgc gaagttacct
     2161 catagtgtct atgcgaaaac tttagtcaag atgctattaa tcatgcacac ccaaaaagcg
     2221 taaatgatcc aaggtcgatt tcgattcccc ttcctttttc tcgctctcct gggattgatt
     2281 gctgctatgt gggcaggctg gattcgcctg ggctgggatt ggccacccat ccagccatct
     2341 cttcccgctt cacacggccc attgatggta gtaggcttcc tgggaactct gatcgccttg
     2401 gagcgtgcag ttgcccttcg ccaaagatgg atgtactttg gaccactcct aagcggtata
     2461 ggtggattag taacgatcat tggcattcca ggacttctag gaccattctt gatggtgctg
     2521 ggcagcctgg ttcttgtcgc aattctcatt gttattttac gccagcatat agcgttgcat
     2581 acggttgcaa tggcattggg tgcgacaagt ttagctattg gcaccatcct tttgtttatt
     2641 ggttggtcga ttcctcgaat tgtgctctgg tgggaggctt ttctcgtttt aaccatcgcc
     2701 ggagagcgtc tggaattagg ccgcttacta aaactctcaa aaggtattgt atgggcattt
     2761 tcagcagtca ccgccctgtc tctgctgggc ttggcaacct caacaatttc gcttatctgg
     2821 ggaacaagaa tttttggctt gggattgctt ggcatggctg catggctgtt gagatatgac
     2881 attgcacggc ataccatccg gcaaaatggc ttggctcgat tcgcagctct ttccatgctc
     2941 tcaggatacg gatggttagc cattggcggc ctgcttgctc tgatatatgg cggtcaagct
     3001 gctggacctg tgtatgacgc cctgttgcac tcggtgttta tcggttttgt gatgacaatg
     3061 attttcgccc acgctccgat tatcttcccc tccattctgg gattgcccat ctcgtttagt
     3121 cgcttgtttt acacccacct gatcttactc cacgcttcgc tgttgttgcg tattatcggc
     3181 gatttagcaa tggtaatgcc tttgcgccgc tggggaggtc ttctaaatgg tatcgcaatc
     3241 ctcacctttc tcgtcatcac gattgtttca atgctcacca atcgagaaag aagcatataa
     3301 aatctaacag gaggtgtcta tgcgcatttt ggcgattata tctggtgatt acggcactcg
     3361 tcacgtcgaa aatatccgca aatttggtcc cccatcctgg accatcaaca cctggcaggc
     3421 tccagctgca tttcctctga ttatagatta cccggaggac tatattcccc aggaactgcc
     3481 cgaaactgac ttggtgctgt catttgctga gcatcgtggc gttgccgagc tcttgccaga
     3541 tattgccaag atgacaaatg cgaaagcggt tgttgcctca atagacaacg aatcttggct
     3601 gccgcgcggc cttgctcggc agctacgggg ttggctggca gatatcgggg tggcgtgcgc
     3661 cacaccaaaa ccactctgtt ccttgacaga aaatgatttc ggcgtcacac gccgacaaag
     3721 aataaaattt gagagtccgt atattgcgga attcgcaacc tgttttggtc aaccttccct
     3781 cattattcag gttgaccccc aatctcgcat tattcaaaaa gctgaggtcc gtcgcgatgc
     3841 cgtatgtggt tgcgcgcgct atgttgccga aagattagtg ggtatctcgg cagatgatgc
     3901 tagcgagaag gctgggcttt tgcatcatca ctatccatgt ctagcaagca tggggaaaga
     3961 cagcgatttt ggcgacactc taatgcacgt gtctggcaat gtcctcaagg acaatgttga
     4021 agtccaggtc cgaccataca aacgtatcca atatatttcc cccggcacaa gaagcgaatg
     4081 agcgaaacca gtaagaaacc aacactacct gattacgatt tggtcttacg aactcgcttt
     4141 ataagcttta gtccgataat aaaggatgcc agcgttcgca acccagccac gataatcaag
     4201 attacgctta aaaatcctgc agccacaatt aagattattt gctttgtact tcttttagac
     4261 ccgctttctg cttggcggat ttgcgctaac aatttctcgc gaagatcgcg cacaaactcc
     4321 tctggtggcc tcaccggctt gagtgtagcc tccagacggg cttcaaaatc acgaacatcc
     4381 tttatattgc cctttaaaaa cgacctcgcc attttataca cttaccctta ttcttaacgt
     4441 tacactacct atcttgttat tcataattgc tttcagtaag tgcatccgtc atcagaagct
     4501 catattcatc ccgtaaggcg agaagagttc ggtgatacaa gctttttaca gcgccctctg
     4561 agcggcccat aatttggccg atctctgcat ttgacatatg ctcaacaaat ttaaggatca
     4621 ataattgctg gcgctctgct gaaagtcgac gaatgatccg tataagccta tcacgctcct
     4681 caaacttaag cagatcatgt tctggatgac tgctatcgaa tcgaccgtgc acgctctctt
     4741 caaggggaat ttctttccgg cgactattat ctctatgcca gtttgctacc agattgtgag
     4801 caatgcggta aagccacgca gatatcggta agccacgatc gttataattt gagatgtgtc
     4861 gcatcgcacg gtagaatacc ctagccgtta aatcttccgc atcatgagca ttgcccgttc
     4921 gataataaat atagttgtaa atccggctta catagcgctc ataaagcgcg ccgaaggctt
     4981 ctccatcgcc atttgctgcc ctggctagaa ttgactcatc gctgagttca gacacagttt
     5041 tcacctagtg gcagcagggt taataataaa aaccctgccg gttcaataaa gttaccattg
     5101 agtataacat caacgctgac aaatatgcaa agcgcgtgct atacagggca attgcatatg
     5161 ccttgagggg catcccgcaa aaagagtgtt tgggctggac tttggctctt taaccaaaat
     5221 ccgaaaggta aaggttgaat attgagcccc aaaatagcga caaaccaggt caaatcctgg
     5281 ctgcggcatg cacagcattg tgcaaaccgg ccaaccag
//
LOCUS       DBNGSX010000410         2950 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1492228, whole genome shotgun sequence.
ACCESSION   DBNGSX010000410 DBNGSX010000000
VERSION     DBNGSX010000410.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1492228"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="AC16CB_12140"
     CDS             complement(<1..532)
                     /locus_tag="AC16CB_12140"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HIR3343265.1"
                     /translation="MDKKKRLQFGLIIFVGFVIWNSLAPRFIYLILPGVSLLLRFLLD
                     KGLFVVLLLAAMNKASSLKYFGIERGVNWWFVLPGLPFILLTGVVLLDPNAAYDLSIT
                     AAVGWVLVGLFVGIGEESLFRGVLWRAFEARGVWTTAFATSAMFGTLHLFGLFVDYVP
                     WKIAASQAAWAFGAGMV"
     gene            682..1908
                     /locus_tag="AC16CB_12145"
     CDS             682..1908
                     /locus_tag="AC16CB_12145"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3343266.1"
                     /translation="MDEIYARKEKNLFLPGYIFFTILLGGLGIYGFYINWKANQPVLA
                     ALSAILVTAHIGLYWLNLRQFHHPRWWVFYYSAQTILIIVIATLPYSGEASLFGISFL
                     GTATISITGEILGLWGNTRRAFLLGLFYLSILLVIIFSKVEPDLILPYLAQFLTTIGF
                     IVILMVVFNLQIRERQKAEELAETLESANAKLAAYAARNEGLTLQAERERMARELHDT
                     LAQGVAGLILQLEALKEHQQQNRYPQAQSVLGQAITRARSTLGESRAAIENLRNDKVD
                     FKEAVEAMIAQTKSVSKVDYQLTLNLIENRPIPQNIQHHAERVLHEALTNINKHAGAA
                     QAQINIEQKQSTLLLHITDDGIGFDTNQPYAEGCFGLQGLAERARLTHSQYMLTSTPG
                     AGTTIEFIFPLERESL"
     gene            1905..2543
                     /locus_tag="AC16CB_12150"
     CDS             1905..2543
                     /locus_tag="AC16CB_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016820417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3343267.1"
                     /translation="MSIRILITDDHLIVREGLRMIFDTVPNIELVGEASNGQEALDAV
                     EALHPDVVLMDLQMPVMDGITAIKHLRQMHPELAIIILTTYKEDALMHRGLAAGAHGY
                     LFKDTDRETLIDTIQAAVQGKTLLSPEVLQQVLNYQSSIPASPSNAESLLTDREAEVL
                     LMVADGARNKEIAYKLGITERTVRAHLNHIFQKLEVDSRSAAAAEGVRRGIV"
BASE COUNT          832 a          737 c          657 g          724 t
ORIGIN      
        1 acaccattcc cgcgccgaat gcccaagccg cctggctggc ggcaattttc cagggaacgt
       61 agtcaacaaa gagaccaaat aaatgcagcg tcccaaacat agcggatgta gcaaaagctg
      121 tggtccacac gccgcgtgcc tcaaatgcgc gccacaatac gccgcgaaaa agactctctt
      181 ctccgatccc cacaaaaaga cctacgagaa cccagcccac cgccgcagta atgctgagat
      241 cataggctgc gttagggtcg agtagtacaa cccctgtcaa taagatgaaa ggcaatcctg
      301 gcaacacgaa ccaccaattt accccgcgct caatcccaaa atattttagg ctgctggctt
      361 tgttcatggc agccaaaagc agcacaacaa acaggccttt gtcgagtaga aatcttagca
      421 gcaaacttac accaggtagt atcaggtata taaatcgggg cgcaagtgaa ttccatatca
      481 caaacccaac aaagatgatc aaaccaaact gtagtctttt tttcttgtcc attgtatcct
      541 ccgaaaatag ttttttgatt tcccccagtg tatcgatttc ctcataaatg cgcatcgtcc
      601 ctttttcccg cgcagcaggt acaacacgac attgtccttt ccgccaatat caaattcaaa
      661 aaaatacagt acaatcaaaa catggacgaa atctacgctc gcaaagagaa aaatcttttt
      721 ctccctggct atattttctt caccatcttg ctgggcggtt taggaatata tggcttttat
      781 ataaactgga aagccaatca gcccgttctt gccgctttga gcgcgattct tgtgacggcc
      841 catattgggc tttattggct caatctcagg caattccatc acccgcgttg gtgggtcttt
      901 tattacagtg ctcagaccat tcttatcatc gttatcgcca ccctccccta tagcggagag
      961 gcaagcttat ttggcattag ttttcttggt acagcgacca tctcgatcac cggggagatt
     1021 ttgggcttat ggggcaatac ccgccgcgcc ttcttgctgg ggttgtttta tctcagtatc
     1081 ctgctcgtga tcatcttttc aaaggttgaa cctgatctca ttctccctta cttagcacaa
     1141 tttctcacaa ccattggctt cattgtaatt ctcatggtcg tgttcaatct gcaaattaga
     1201 gaacgccaaa aagccgaaga gctggctgaa actctggaga gcgccaatgc aaagctggct
     1261 gcttacgccg cacgtaacga agggctgacc ttgcaagcag aacgcgaacg catggcgcgc
     1321 gagttacacg acactcttgc acagggtgtt gctgggttga tcttacaact tgaagcactt
     1381 aaggaacacc agcagcaaaa ccgctacccg caagcacaat ccgtgcttgg gcaagctatt
     1441 actcgtgctc gcagtactct gggtgagtcg cgcgctgcca tagaaaactt gcgcaatgat
     1501 aaagtagatt tcaaagaggc tgttgaggct atgattgcac agacaaaaag cgtgagcaaa
     1561 gttgattatc aactgaccct gaatttaatc gaaaatcgcc ccatacctca aaatattcag
     1621 caccatgcag aacgtgtgtt gcacgaagcg ctgaccaata tcaacaagca tgctggggcc
     1681 gcgcaggcac agatcaatat cgagcaaaag caaagcacac tcttgctgca catcaccgac
     1741 gatggtattg gttttgacac caaccagccg tatgcagagg ggtgctttgg tcttcaaggg
     1801 ctggcagagc gcgcccgtct gacccacagc caatacatgc tgaccagcac accaggagca
     1861 ggtacgacga ttgaattcat ctttccatta gagagggaat ccctatgagc atccgaatac
     1921 ttattactga tgaccatctc atcgtccgcg agggactccg tatgattttt gatactgtac
     1981 cgaatataga gttggtcggt gaggccagta atgggcagga agcgctggat gcagtagagg
     2041 cgcttcaccc agatgtggtg ctgatggatc ttcaaatgcc ggtcatggat ggtattacgg
     2101 ctatcaaaca tctgcgccag atgcaccctg aattagcaat tattattctg actacgtata
     2161 aagaggatgc ccttatgcac cgtggcctgg cagcaggtgc acatggctat ttattcaaag
     2221 atacggatcg cgaaaccttg atcgacacca ttcaggctgc agtgcaaggc aagactttgc
     2281 taagccccga ggtattacaa caggtactca actaccaaag cagtatcccc gccagtccgt
     2341 ccaacgctga aagcttgctt actgaccgcg aagctgaagt cttattaatg gtggctgatg
     2401 gggcacgcaa taaagagatt gcctataaac ttgggattac cgagcgcacc gtaagagcac
     2461 atctcaatca tattttccag aaattagaag tggattctcg ctctgctgct gcagccgaag
     2521 gggttaggcg agggatagtg taagtcaatt tgacaaatct gattcagtga tctataaatc
     2581 ccaagatttt cttagactta gattcaaacc tcaatatagc aaggaaccgc tcaacatcat
     2641 ctgttggctt cctgaatgca cccgtagagt ttggctaagc caaactctta aacataagac
     2701 cagagatcag ttgatctctg gtcgttgtca gtagcgggta gcggacctgt tccgaaccac
     2761 tcccttccct atttttaggg ggaaataaga cgctgaagcg tcacattttc aagaatctta
     2821 gtctgaagtg catgagtcgt caaggaacac tgatgttact tactagtata aagaagtaat
     2881 gaggtatcct ccttgtacaa gaatagtgac atattcaaaa caaggaggaa cctcatgtct
     2941 agaaaaaaca
//
LOCUS       DBNGSX010000411         2880 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1422670, whole genome shotgun sequence.
ACCESSION   DBNGSX010000411 DBNGSX010000000
VERSION     DBNGSX010000411.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1422670"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="AC16CB_12155"
     CDS             complement(<1..268)
                     /locus_tag="AC16CB_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylyltransferase domain-containing protein"
                     /protein_id="HIR3343268.1"
                     /translation="MPGKNYRPLGSKPLFHYIIESLLNCPEIDRVVVDTDSPPVMDGL
                     RQHFPKVTLIERPEHLRADETPTNEVLLHDTAQVQADFYLQTHST"
     gene            complement(317..1252)
                     /locus_tag="AC16CB_12160"
     CDS             complement(317..1252)
                     /locus_tag="AC16CB_12160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3343269.1"
                     /translation="MISIVIRAYNEERHIGRLLEGIAQQNLGETEIILVDSGSTDATL
                     EIARRYALRVVHIQPDEFTFGRSLNRGIAASQGEIIVIASAHVYPVYPDWLERLLAPF
                     SDPQVALAYGKQRGNATAKFSEGQIFARWYPESSDLQQAHPFCNNANAAIRRSLWQLH
                     LYDESLSGLEDLDWAHWAVGEGYRLAYVAEAEVIHVHNETPRAVYNRYRREAMAFKRI
                     FPQEHFGLRDFLRLSLSNIFSDMWHAARQGVVLRSLVSIFWFRLMQFWGTYQGYRHLG
                     PLTWQLRQTFYYPRGIAPTGQAAARNVEPIRYNEQ"
     gene            complement(1286..2140)
                     /locus_tag="AC16CB_12165"
     CDS             complement(1286..2140)
                     /locus_tag="AC16CB_12165"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015016 - heparan sulfate
                     N-sulfotransferase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HIR3343270.1"
                     /translation="MNGKTRLRLARRYLRRMAQSARWRRTLRGVPILFANSFPKSGTH
                     LLTQVLQGFPRLGPAVDSGLPAIVTFEGDTGRTRQPQEILADLQRLLPGDIAYGHVHA
                     LPQVVDFICREGVCAYFILRDPRDVAVSHVHYVTEMEPGHAHHRYYTEELHSFDERLQ
                     VSIQGIPGTDVQFPDIRARFEPYLGWLERPEVLTLRFEDFISQRMDTLGRVFDHAVQR
                     GFPASAERQDAIQTLAQVIDPHSSPTFRSGKAGAWRENFNSENKRLFKDVAGDLLVRL
                     GYEQDHDW"
     gene            complement(2130..>2880)
                     /locus_tag="AC16CB_12170"
     CDS             complement(2130..>2880)
                     /locus_tag="AC16CB_12170"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015016 - heparan sulfate
                     N-sulfotransferase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HIR3343271.1"
                     /translation="LGNAMPKSGSHLIIQVLQGLTSLGPVVTPGFPPVNRGEDNRKLS
                     DRAVLANIQRMLPGDIGYGYISATPTFIDALTRPRRATIFVYRDPRDMIVSHIFYATQ
                     MHPDHWMRRYYTETLSTMEERINAAIQGVDEPGSELTPIRRRYEGYLAWLEQPEVLSL
                     RFEDLVLNRQAVLKQLLDFIRGRGLNLKMPRVQAVSILDASVAPKKSGTFRKGQPGNW
                     REHFTDANKALFKEKTGDLLTRLGYETSDEW"
BASE COUNT          540 a          811 c          927 g          602 t
ORIGIN      
        1 ttgtgctgtg cgtctgcagg taaaaatcgg cttgcacctg ggcggtgtcg tgcagcagca
       61 cttcgttggt gggggtctcg tcggcgcgca ggtgctcggg gcgctcgatg agggtcacct
      121 tggggaaatg ctggcgcaat ccgtccatca cgggcgggct gtcggtatcc accacgaccc
      181 gatcgatctc tgggcagttt agaagactct cgataatgta gtggaagagc ggcttgctgc
      241 caagcgggcg gtagttctta cccggcacac gctgcgagtg gtggcgcata gggacgaggg
      301 cgatgagggt catagattat tgctcattat accgaatggg ctccacattc cgcgcagctg
      361 cctggccggt tggcgcaata cccctgggat aataaaatgt ctgccggagc tgccaggtga
      421 gcggcccaag atggcggtag ccctgatagg tgccccagaa ctgcatcagg cggaaccaga
      481 agatgctcac caggctgcga agcacgacgc cttgccgtgc ggcgtgccac atatcgctga
      541 atatgttgct caggcttagg cgcaggaagt cgcgcagacc gaaatgctcc tgggggaaga
      601 tgcgtttgaa ggccattgcc tcccggcggt atcgattgta caccgcgcgt ggcgtctcgt
      661 tgtgcacgtg gatcacctcc gcttcggcta cataggccag cctgtacccc tcgcccactg
      721 cccagtgtgc ccaatccagg tcctctagtc cgctcaggct ctcatcatag aggtgaagct
      781 gccagagcga acggcggatg gcggcgttgg cgttgttgca aaaggggtgc gcctgctgca
      841 ggtcggacga ttccgggtac cagcgggcga agatctgtcc ttccgagaac ttggcggtgg
      901 cgttgccgcg ctgcttgccg tatgccagcg ccacctgggg gtcgctgaaa ggagccagta
      961 gtcgctccag ccagtcggga tagaccggat agacgtgtgc gctggcgatg acgattattt
     1021 cgccctgcga ggcggcgatg ccccggttaa gcgagcgccc gaaggtgaac tcgtctggct
     1081 ggatatgcac cacgcggagc gcatagcgcc gggcgatctc cagcgtggca tctgtggagc
     1141 ccgaatcgac caggatgatc tcggtctcgc ccaggttctg ctgcgcgatg ccttccagca
     1201 gtcgcccgat gtgtcgctcc tcgttatatg ctcggataac aatagatatc atttctaagt
     1261 gctgttcgct gattgctggt ttactctacc agtcgtggtc ctgctcgtag cccaggcgta
     1321 ccagcaaatc tccggctaca tctttgaata aacgcttgtt ctctgaattg aaattctccc
     1381 gccaggcgcc agccttaccg ctgcggaaag tgggtgagct gtgcgggtcg atgacctgtg
     1441 ccaaggtctg gatggcgtcc tggcgctctg cggatgcagg gaagccgcgc tgcacggcgt
     1501 ggtcgaagac gcgtcccaga gtatccatac gctgactgat gaaatcttca aagcgcaagg
     1561 tcagcacctc ggggcgctcc agccagccca ggtaaggctc gaagcgagcg cggatatctg
     1621 ggaattgaac gtctgtcccg gggatgccct gtatgctgac ctgtagccgc tcgtcgaaac
     1681 tatgcagctc ttctgtgtag tagcgatggt gggcgtgccc tggctccatc tcggtgacgt
     1741 aatgcacgtg cgagacagcc acatcgcgcg gatcgcgcag gatgaagtat gcacacaccc
     1801 cctcccggca gatgaagtcc acaacttggg gcagggcgtg cacgtgcccg taggcgatgt
     1861 caccggggag caggcgttgc aggtcggcga ggatctcctg gggctggcgg gtacgccctg
     1921 tatccccctc gaaggtcacg atggcgggca ggccgctatc cacggctgga ccaagccttg
     1981 gaaagccctg caagacctgg gtcagcaggt gcgtgccgct ttttgggaag gagttggcaa
     2041 acaagatggg cacacctcgc aaggtgcggc gccagcgggc gctctgtgcc atgcgcctca
     2101 agtagcggcg ggccagcctt aatcttgtct taccattcat cgctcgtctc gtacccaaga
     2161 cgggtgagca aatccccggt tttctccttg aatagggctt tgttggcatc cgtgaagtgc
     2221 tcgcgccagt tgcccggctg ccctttgcgg aaagtaccgg acttctttgg ggcgacggag
     2281 gcatccaaaa tgctcaccgc ctgaacgcgc ggcattttca aattaagacc gcggcctctt
     2341 atgaaatcca gcagttgttt caatacagcc tggcgattca agaccaggtc ttcaaagcgc
     2401 aggctgagca cctcgggttg ctcaagccaa gccagatagc cctcgtagcg ccggcggatg
     2461 ggggttagct cggagccggg ttcgtccacg ccctggatgg cagcattgat gcgctcctcc
     2521 atcgtggaca gcgtttcagt gtaatagcgg cgcatccagt ggtctgggtg catctgtgtg
     2581 gcgtagaaga tgtgcgagac gatcatatcg cgcgggtcgc ggtatacaaa gattgtagcg
     2641 cgcctgggtc gggtgagtgc gtcgataaag gttggcgtgg cgctgatata gccatagccg
     2701 atatcgccag gtagcatgcg ctgaatgttc gcaagcactg cacggtcact cagcttgcgg
     2761 ttatcctcgc cgcggttgac gggcgggaag cctggagtga cgactgggcc taagcttgtc
     2821 agcccctgca gcacctggat gatcaggtgc gagccggact ttggcatggc gttgcccagc
//
LOCUS       DBNGSX010000412         3650 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_453224, whole genome shotgun sequence.
ACCESSION   DBNGSX010000412 DBNGSX010000000
VERSION     DBNGSX010000412.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_453224"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1668
                     /locus_tag="AC16CB_12175"
     CDS             <1..1668
                     /locus_tag="AC16CB_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343272.1"
                     /translation="GLHAQYGEKASVLTAEMQRLAQAVGARRDWRSVLFFADEGLPPD
                     VRSARPSDPWAIKLALADLDAHLGCKGEMIGAVLIVGGPEVVPFHHLPNPVDDADDYV
                     PSDNPYATRDENYFIPEWPVGRLPGGVAKDPSGLQNILKSLTDQHIALAKHSKPRNSP
                     WWIAWLNWLLRRQAEPFNSKHKRPSFGYTAEVWRKASQIVFRPIGEAKALRVSPPLSA
                     SGQSMVPLPSGKLAYFNLHGLVDAVEWYGQSEAVTSSGNGSGVPNEDQRDYPVAIRPQ
                     DIVNSGHAPQVVFSEACYGAHILGKSIEEALALKFLQAGSQAMVGSTCTAYGSINTPL
                     TAADFLGHAFWSALKQGSPAGEALQRAKVSLAREMHRRQGYLDGEDQKTLISFVLYGD
                     PLAQPLGQVRQAKGKFRPAVLRPLKPPRGVKTICDRVRLEDQENPLPAEVVDYVKSIV
                     EQYLPGMKGAQVSFSQEHIDCNMDGHQCPTGQLHMKHHGKSATSSSSPASAQTSRGMN
                     SNAIPPTQQRRIVILSKVVGNAAHVHRHYARLTLDEQNRLVKLVVSR"
     gene            1933..3423
                     /locus_tag="AC16CB_12180"
     CDS             1933..3423
                     /locus_tag="AC16CB_12180"
                     /inference="COORDINATES: protein motif:HMM:NF024748.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR3343273.1"
                     /translation="MRNRSIFSPLRWFSAFFLLAALVLSAVQLARYSRVRAYFPPGLK
                     IAGIPVGGMDRQQAAQRLLEAYSTPVELIYNNSPIQITPGVLDFQLKLEDMLAAADLQ
                     RTQQPFWQGFWDYLWGRASTPIEVPLRSSHSEARLRSYLEDEISKRYDQPPVPPSPVV
                     GTVNFQPGSPGTELDVENAIPSIVSAARSLSLRQVVLPLQRSEPPRPAFQNLKVLLKQ
                     TIDLTEFDGLTGIYLLDLQTAQEIHMEYMQGEELSLQPDVAYTASSMIKIPIMISVYR
                     RLGENPDSETLKLLNDMITKSGNETADWLMDRVMEPGRAPLIVSEDMQALGLQNTFMA
                     GYFTLGSPLLVRYETPANQRADINTDPDPYSQTTPTEIGMLLEDIYQCAISGGSALQA
                     VYAGEITQGECQAMIDYLKNNHLPALLTAGIPEATQIAHKHGWVSDLNGVINTIGDAG
                     IIYTPGANYVLVIFLHHPQQLVWEQATTMVADLSRAIYNYYNLPSQ"
BASE COUNT          900 a         1041 c          926 g          783 t
ORIGIN      
        1 ggattgcacg cccaatacgg agaaaaggct tctgtcctta cagctgaaat gcagcgacta
       61 gcccaggcgg tgggggcgcg tcgagactgg cggtctgtgc tcttttttgc cgatgaaggt
      121 ctgccacccg acgtacgatc tgctcgccca tccgaccctt gggcgatcaa actagccttg
      181 gcagacttag atgcccactt gggatgtaaa ggcgagatga tcggtgccgt gctaattgtg
      241 ggtggaccgg aggtcgtccc attccaccat cttccaaatc cggtggacga tgctgatgat
      301 tacgtgccct cagataatcc ttacgccaca cgtgatgaaa actacttcat acctgaatgg
      361 cctgttgggc gtcttccagg cggcgttgca aaagacccca gtggattgca gaacatatta
      421 aaatcgctaa ctgatcagca cattgcctta gcaaaacaca gcaagcccag gaacagtcct
      481 tggtggatcg cttggttgaa ctggctacta cgcaggcaag cggaaccttt caactccaag
      541 cataagcgcc ccagctttgg ctatactgct gaggtttggc gcaaggcttc gcagatcgtt
      601 tttcgaccta ttggggaagc taaagctctt cgagtttcac cccccctcag cgcaagcgga
      661 caatctatgg tgcctcttcc ctctggcaag ttagcttact tcaacctgca tggtctggta
      721 gatgcagtgg agtggtacgg gcaaagtgaa gcagtcacat cttctgggaa cggatcgggc
      781 gtaccgaacg aagaccaacg cgactaccca gtcgccattc gcccacaaga cattgtcaac
      841 agcgggcacg cacctcaagt ggtcttcagc gaagcttgct atggagcgca tatattgggg
      901 aagagcattg aagaggcctt ggcactcaag tttttacaag cgggcagcca ggcgatggtt
      961 ggctccacat gcacagccta cggatcaatc aatacccccc taactgccgc cgacttcctg
     1021 ggtcacgcct tctggagcgc cctcaaacag ggcagcccag ccggggaggc tctgcaacgg
     1081 gcgaaggtct ccttggcacg cgagatgcac cgccggcaag gttatttaga cggcgaagat
     1141 cagaaaacct tgatctcctt cgtgctgtac ggagacccat tagcccaacc tttagggcag
     1201 gtacggcagg cgaagggaaa gttccgccca gcggtgctgc gcccgctgaa accgccgcgt
     1261 ggcgtaaaga ctatctgcga tcgagtgcga ttagaggatc aggaaaaccc cctaccggct
     1321 gaggtggtgg attacgtcaa atccatcgtc gagcagtact tacccggcat gaagggagcg
     1381 caagtatctt ttagccaaga acatatagat tgcaacatgg atgggcacca atgccccaca
     1441 gggcaattgc acatgaaaca tcatggaaag agtgcaacat caagttcctc gccagcatca
     1501 gctcagacat ctcggggtat gaactctaat gcaataccac caacccaaca aagacgcatt
     1561 gtgatcctaa gcaaagtggt tggtaacgca gcccacgtgc accgccatta cgcccgctta
     1621 accctggatg aacaaaatcg attagtgaag ctggtggtct ccagataaat tgtcagcgat
     1681 atattcgagc gaagatgcag gctcttcgta atccccgctg cgggtggcaa gatttgagca
     1741 gggcaattgc aatataccct gaggggcatg cgcaatcccg taaaaagagt gtttggggtt
     1801 ttttgttggg ggcatacccc caccaaaaca ccccattttc tctcctcagt ggtgggatat
     1861 cgcccaaatg cgattgcctt atttgagcgt gcagtaaaat gactccatgg tttaatggta
     1921 tgatagcaag ccatgagaaa tcgcagtatt ttttctccac tgcgctggtt ctcggcgttt
     1981 ttcctgctcg cagcgctggt gctctcagct gtccaactgg cgcgttatag tagggtacgg
     2041 gcatacttcc ctcctggcct aaaaattgcc ggcatacccg tgggtgggat ggatcgacaa
     2101 caagcggccc agcgcctatt ggaagcgtat tcaactccgg tagaactgat ctacaacaac
     2161 tcccccatcc agatcacccc cggcgtgctc gattttcagc tcaagctgga ggatatgctg
     2221 gcagccgccg atttacagcg cactcagcaa cctttctggc aaggcttctg ggattaccta
     2281 tgggggcgag catccacgcc aatagaagta ccactgcgct cgtcccattc cgaagcgcgc
     2341 ctgcgctctt atttggaaga tgagatctcc aaacgctacg atcaaccccc agtccccccc
     2401 agcccggtcg taggtacggt aaattttcaa cccggctctc ctgggacaga attggacgtt
     2461 gaaaatgcga tccccagcat tgtgagcgca gcgcgatcgc tttcgctacg ccaggtcgta
     2521 ttgccgcttc aacgttccga gcccccgcgt cccgcattcc aaaacctcaa ggtgctgctc
     2581 aagcagacca tcgacctgac agagtttgat ggtctgacag ggatttacct gctcgatctg
     2641 caaaccgctc aggaaatcca catggaatac atgcaagggg aagagttatc cttgcagccc
     2701 gatgttgcat acaccgcctc cagcatgatc aagataccca tcatgatatc cgtgtatcgc
     2761 cgtctgggag agaaccctga ctcagagact ttaaagctgc tcaatgacat gatcaccaag
     2821 tctggaaacg agacggcgga ctggttgatg gaccgcgtga tggagccagg tcgagcgccg
     2881 ttgatagtaa gtgaagatat gcaagccttg ggattgcaaa acacctttat ggcaggctat
     2941 ttcaccctcg gctcaccgct actggtacgc tacgagaccc ccgcaaacca acgtgcagac
     3001 atcaacaccg accccgaccc ctacagccag accacaccca cggagattgg catgcttctg
     3061 gaggacatct accaatgtgc tatctcaggg ggcagcgcgc tgcaggcagt ttatgctggc
     3121 gagataaccc agggtgaatg tcaggcaatg atcgattacc tgaaaaacaa tcatttgccc
     3181 gccctgctca ccgcgggcat acctgaagcc acccaaatcg ctcacaagca cggttgggtt
     3241 tcggatttaa acggcgtgat caatactatc ggcgacgcgg ggatcatcta cacccctggc
     3301 gccaactacg tactggtgat cttccttcac catcctcaac aattggtgtg ggagcaagcc
     3361 accacaatgg tagccgacct atcgcgggcg atttacaatt actacaatct gccaagccag
     3421 taaattctct acactcaacc gcctcaataa ccctagaatc cagtagcgaa gcagacagtg
     3481 aaacccaaca cgaaaaaggg tttagccgtt ctacccctaa cctctttctt tcagatactc
     3541 gcgaatcatg atggcagccg tcgccccttc cccagcagca gaagcggcct gcttggtcga
     3601 acctgctcgc acatcgccag cggcgaataa gcccggcttg ctcgtcatca
//
LOCUS       DBNGSX010000413         5645 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1492358, whole genome shotgun sequence.
ACCESSION   DBNGSX010000413 DBNGSX010000000
VERSION     DBNGSX010000413.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5645)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1492358"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="AC16CB_12185"
     CDS             <1..791
                     /locus_tag="AC16CB_12185"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR3343274.1"
                     /translation="PIAEVQYLDYVLYALQILSDDLATLHWRTKGGQKAPVILRTRGH
                     RLEGIWHSGSPMAGLIHLVRGMHVLVPRDMTRAAGFYNTLLRSDDPAVVIEVLNGYRV
                     RERLPENIGEVRVPLGVPEAIREGTDVTIVTYGACCRIALEAADLLSKVDIQAEVIDV
                     QTLLPFDIHGRILESLKKTSRILFVDEDVPGGTTAYMFQEVIEKQGGYYWLDSPPRTL
                     SGKPHRPAYGSDGDYWSKPNAEQIFEAVYEMMNEVDPGSYPIFY"
     gene            965..2227
                     /locus_tag="AC16CB_12190"
     CDS             965..2227
                     /locus_tag="AC16CB_12190"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIR3343275.1"
                     /translation="MPVQVLMPQLGESVLEGTITKWLKAKGERVGEYEPLLEVNTDKV
                     DTEIPSPADGVLLEILVEEGTTVQAGSLLAWIGEAGESLPAQGTLPEIELATPPAESA
                     QPAVVTTAPAAGRDRDLGFISPVVARMAAEHNIDLSKVPGSGQGGRITKKDMQAYIET
                     MKVEGEGRPTPVVSPPLPAVSPIPSPPAPGFPTSQPGDILPMTAIRRAIADHMVMSKH
                     TSPHVTTVMEADMSRVATHREANKAAFARDGVNLTFTAYLVSASVSALRAFPIANSSW
                     SDEGIRLHRQINIGMATSLGEEGLIVPVIKNADGLSLLGLAREVNDLSNRARAKKLQP
                     DEVKGGTFTITNHGTSGSLFATPIINQPQCAILGVGIIQKRVVVINDAIAIRPMVYLS
                     LTFDHRILDGAIADYFLAKVVETLQNWG"
     gene            2299..3696
                     /gene="lpdA"
                     /locus_tag="AC16CB_12195"
     CDS             2299..3696
                     /gene="lpdA"
                     /locus_tag="AC16CB_12195"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR3343276.1"
                     /translation="MSDYDVIVIGAGPGGYVCAIRAAQLGLKTAIIDKQWLGGVCLNV
                     GCIPSKALLKNAEVAHTLRERGKELGFSFENLTLDYSVAVKRSRQVSERLVKGVGFLM
                     KKNKIDVHMGAARVTASDTVAVTDKDGNTVELKTKNIVVATGASVAVPPGWEIDGEKI
                     VTYLEAILQEKLPKSVIIIGAGAIGVEFATVWNSYGVDVTIVEMLPRLVPLEDEEVST
                     ELTRAFNKRKITSLVGHRVEAIAATKGGVQVKVSSEAGEKTLEAEQALVAIGFTPNGR
                     GLGLEEVGVKLSQRGAVEIDERMATNVPGIWAIGDVTAKLMLAHVGSAQGIICAENIA
                     GAETVTLDYEMMPRATYSQPQVASFGMTEAQAKERGYDVKVGRFPFQASGKALGMGES
                     AGWVKILTDAKYGEILGAHMVGPEVTELLPELTIAQMMELTTAEIARNVHAHPTLSEV
                     LMEAAHGAEGHAINI"
     gene            complement(3752..4981)
                     /locus_tag="AC16CB_12200"
     CDS             complement(3752..4981)
                     /locus_tag="AC16CB_12200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3343277.1"
                     /translation="MMYPRFAMRILFVVDGRSPIALNWIRYFSQSDHEVHVVSTYPCS
                     PDLRLASLNVVPTAFGGVGESKSKAEGIEDRGLLRRLVPVGARTALRQWLGPLTLPAA
                     ARDLARLIKQIQPDLVHAMRIPYEGMLAALALGEAGIPRLLVSVWGNDFTLHARATPR
                     MASLTRLVMRRATALHADCQRDIRLARRWGYAENKSVAVLPGGGGVQADIFYPDKEQS
                     NKQIGGRGEGAMVINPRGFRAYVRNDAFFRAVPLVLERMPETRFVCPAMAGEAQALNW
                     LETFDIGHAVDLLPLQTRPEMAELFRRCQVAVSPSTHDGTPNTLLEAMACGCFPVAGD
                     IESLREWINPRVNGLLVDPRSPSALSEAILRALRDPEMRLRAREENTRLIAERAEYKW
                     VMGQADRYYQRVFSNGR"
     gene            complement(5014..>5645)
                     /locus_tag="AC16CB_12205"
     CDS             complement(5014..>5645)
                     /locus_tag="AC16CB_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007356366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR3343278.1"
                     /translation="DNLRTQHKYHNLTRRDQLPGYEAAIRRLHDLGVMVNASFVFGMD
                     GDDPDVFDRSVDWAITQGVETATFHILTPYPGTPLHARLQDQARITSHDWDKYDTRHA
                     VFRPARMDAETLEAGYWRAYREFYRWGSILRGAGSKPDLPGRLRHLAYSAGWKKFEPL
                     WDWVIRARRVAQLLPFLEAILEGFSPREKDTRAVKQIDALGKIGQFGID"
BASE COUNT         1258 a         1555 c         1674 g         1158 t
ORIGIN      
        1 gccctatcgc tgaggtgcag tatctggact acgtgctgta cgccttgcaa atcctatccg
       61 atgacctggc gaccctgcac tggcgcacga aaggcgggca gaaggcgccg gtgatcctgc
      121 gcacgcgagg ccaccggctg gagggtatct ggcactccgg atcacctatg gcggggttga
      181 ttcacctggt gcgtgggatg catgtgctgg tgccgcggga tatgacccgc gcggctggct
      241 tctacaatac gctgctgcgc agcgatgacc cagcggtggt gattgaggtg ctcaacggct
      301 accgcgtccg cgagcgtctg ccggagaata ttggcgaagt cagggtgcca ttaggtgtgc
      361 ccgaagcgat ccgagagggt acggatgtta ccatcgtcac ttacggtgcg tgctgccgca
      421 ttgccctgga ggctgccgac ttgctcagca aagtggatat ccaagcggag gtcatcgatg
      481 tgcaaaccct attgcctttc gatattcacg ggcgcatcct cgagtcactc aagaaaacca
      541 gccgcatcct atttgtggat gaagatgtgc ctggtggcac aactgcatat atgttccaag
      601 aggtgatcga gaagcagggt ggctactatt ggctggactc tccgccccgt accctgtctg
      661 gcaaacctca tcgtcccgct tacggctccg atggtgatta ctggtccaag cccaacgcgg
      721 agcagatttt cgaggcggtg tacgagatga tgaacgaggt ggatccgggt agctatccaa
      781 tattttatta accgcacaga aacagagaac gcaaagattc ttgtatattt aaccgaagga
      841 cacagagtaa aaagatgact tagagtagtg cagtcatgat gaaacaaggt ataaaacgca
      901 tgatatatga tattggagaa ttactccgcg aactctgtgt ctctgtggtg atcttggagg
      961 aacaatgcct gtacaagtct tgatgccaca gctgggtgaa tcggtcctgg aagggacgat
     1021 caccaaatgg ctcaaagcca agggcgagcg tgtgggcgaa tacgagccct tgctggaagt
     1081 aaacacggac aaggtagata cagaaatccc tagcccagcc gatggcgtat tgctggaaat
     1141 cctggtggag gaaggtacca cggtgcaggc tggcagcttg ctagcctgga tcggggaagc
     1201 cggcgagtcc ttgcccgcgc agggcaccct ccccgaaatc gagcttgcca caccgcctgc
     1261 ggaaagtgcc caaccagcgg tggtgaccac ggcacccgcc gcgggaagag accgcgattt
     1321 aggattcatc tcacccgttg tagcgcgcat ggctgctgag cacaacatcg acttgagcaa
     1381 agtcccgggc agcgggcaag gcgggcgcat caccaagaag gacatgcagg catatattga
     1441 gacgatgaaa gttgaaggcg aagggagacc cacaccagtt gtttcgcctc cccttccggc
     1501 tgtatctcca attccttctc ccccagcccc gggtttcccg acgtcccaac ctggcgacat
     1561 cctccccatg acagctatcc ggcgcgccat cgccgatcat atggtgatga gcaagcacac
     1621 gtccccgcac gtaacgaccg tgatggaggc tgatatgagc cgggtagcta cacaccggga
     1681 agccaataaa gccgccttcg ctcgcgacgg cgtcaacctg accttcaccg catatttggt
     1741 ctccgcctcg gtgagcgcgc tgagggcatt cccgattgcc aattcatcct ggagtgacga
     1801 gggcattcgg ctgcatcgcc agattaacat aggtatggca acttcccttg gtgaggaggg
     1861 gctgattgtg ccggtgatca agaacgccga cggcctatcc ctgttgggtc tggctcgcga
     1921 ggtcaatgat ctatccaacc gggcgcgggc caagaagctg caacctgatg aagtcaaagg
     1981 cggcaccttc accatcacca accatggtac cagcggatca cttttcgcca cgccaatcat
     2041 caaccagccg caatgcgcca tcttgggggt gggcatcatc cagaagcgtg tcgtagtgat
     2101 caacgacgct atcgccatcc gcccgatggt ttatctatcc ctgaccttcg accaccgcat
     2161 cctggatgga gcgattgccg attacttcct ggcgaaggtg gtagagacgc tgcaaaattg
     2221 gggatagctg gaagcggaga gtagtaaaat agaatgattt tgagcggata atttattctc
     2281 gttttttcag gagtaaccat gagtgattat gacgtcatcg taattggagc agggcctggg
     2341 gggtatgtgt gtgccatccg cgctgcgcag cttggcctga agactgccat tatcgacaag
     2401 cagtggctgg ggggtgtctg cctgaatgta ggctgcatcc cttccaaggc gctgttaaaa
     2461 aatgctgagg tagcccacac tctgcgcgag cgcggcaagg agctgggctt ttcgtttgag
     2521 aatctcaccc tggattacag cgtggctgtc aagcgcagcc gccaggtctc tgagcggctg
     2581 gtcaaggggg tgggctttct gatgaagaaa aacaagatcg atgtccacat gggggctgcc
     2641 cgggtgacag ccagcgacac ggttgccgtg acggataagg atggcaatac cgttgagttg
     2701 aagaccaaga acatcgttgt ggcaactggt gccagtgtgg cggtcccgcc gggctgggag
     2761 atcgatggcg agaagatcgt cacctatttg gaagccattt tgcaggagaa gttgcccaaa
     2821 tcagtcatta tcataggcgc cggggcgatc ggcgtggagt ttgccacagt gtggaattcc
     2881 tacggtgtgg atgtgaccat cgttgagatg ctgccgcggc tggtgcccct ggaggacgag
     2941 gaggtcagca ccgagcttac gagagccttc aacaaacgca agataacttc actggtcggg
     3001 catcgcgtgg aagccatcgc agccacaaag ggcggcgtgc aggtcaaggt ctccagtgag
     3061 gccggcgaga aaaccctgga agccgagcag gcgctggtgg ctatcggttt cacacccaac
     3121 gggcgcggct tgggcttgga ggaagtgggg gtaaaactca gccagcgcgg cgcagtggag
     3181 atcgacgagc gcatggcgac caacgtgcca ggcatctggg ccatcggcga tgtaaccgcc
     3241 aaattgatgc tagcccacgt tggatcggcg caggggatca tctgcgctga gaacatagct
     3301 ggcgctgaga cagtaaccct ggattacgag atgatgccgc gcgctaccta cagccagcca
     3361 caggtggcct cctttggcat gaccgaagcc caggccaagg agcgcggtta tgatgttaag
     3421 gtagggcgct tccccttcca ggctagcggt aaggcgctcg gcatgggcga atcagctggc
     3481 tgggtgaaaa ttctcaccga tgctaaatac ggtgagatcc tgggcgcgca catggttggt
     3541 ccggaggtga ctgagctgct ccctgagcta accatcgccc aaatgatgga actgacgacg
     3601 gctgagatcg cccgcaacgt ccacgctcat cccaccctta gtgaggtgct gatggaggcg
     3661 gctcatggag cagaagggca cgccatcaat atctaagtcc tatatctggt atccattgct
     3721 ggaacattga aattgggaca ggcgattcat tttatcttcc gtttgaaaaa acccgctgat
     3781 aatatctatc tgcctgcccc atcacccatt tatattctgc ccgctcggcg atcaggcggg
     3841 tgttttcctc ccgggcgcgt agacgcatct ctggatctcg aagggcgcgc aggatcgcct
     3901 ccgacagcgc gctcggggag cgggggtcaa ccagcaagcc gttgactctg ggattgatcc
     3961 actctcttaa tgattcgata tcccctgcca cagggaagca cccgcaggcc atagcctcca
     4021 gaagcgtgtt gggcgtgccg tcatgcgtgc tgggcgacac tgctacctgg caacggcgga
     4081 ataattcggc catctcgggg cgcgtctgta gtggtagcag atcaactgca tggccaatgt
     4141 cgaatgtctc caaccaattc agggcttgtg cctccccagc cattgctgga cagacaaagc
     4201 gggtttcggg catcctttcc agcaccagag gcacggcgcg gaagaaagca tcgttacgca
     4261 cgtaggcccg gaagccgcgc gggttgatca ccatggctcc ctcacctcta ccgccaattt
     4321 gtttgttcga ctgctcttta tctggataaa atatatcagc ctggacccca ccacctccgg
     4381 gcagcaccgc aacggattta ttttcagcgt atccccaacg acgggctagg cggatatccc
     4441 gctggcagtc ggcgtgcaag gcggtggcgc ggcgcatcac cagccgggta agggaggcca
     4501 tacgcggcgt ggcgcgggca tgcaaggtga aatcattgcc ccaaaccgag accaacaagc
     4561 gcggtatacc ggcttccccc aatgccaagg cagccagcat gccctcatag gggatgcgca
     4621 tggcgtgcac caggtcaggc tgaatttgct tgatcagcct agcaaggtct ctggctgcgg
     4681 ccggcagcgt gagtggtcca agccattgcc ggagggctgt cctggctccc accggaacca
     4741 acctgcgcaa caatccccta tcctctatcc cctctgcctt gcttttactc tctcctaccc
     4801 caccaaacgc ggttgggact acgttcaaag atgccaggcg caagtctggc gagcaaggat
     4861 aggtggatac tacgtgaacc tcgtgatcgc tttggctgaa atagcgaatc cagttcaagg
     4921 cgatgggcga tctgccgtca acaacaaaaa gaatgcgcat ggcaaatcga gggtacatca
     4981 tcttggcgga aaatgcaatg ctggagctat gcgctaatca atgccaaatt ggccaatctt
     5041 ccccagagcg tctatttgtt ttactgcacg ggtatctttt tctcttgggc taaatccttc
     5101 caggatggct tccaggaagg gtagtaattg ggctaccctc ctggcgcgga tgacccaatc
     5161 ccacaggggc tcgaattttt tccagccagc cgagtaagcc aggtggcgca acctgccggg
     5221 taggtcgggc ttgctcccgg cgcctcgcaa gatcgaaccc cagcgataga actcccggta
     5281 ggcacgccag tagccagcct ccagcgtctc agcgtccatg cgagccgggc gaaagacggc
     5341 atgacgcgta tcatacttat cccagtcgtg gctggtaata cgcgcctggt cctgcaaacg
     5401 agcgtgcaag ggagtgcccg gatatggagt caggatgtgg aaggtggctg tctccacacc
     5461 ctgcgtgatc gcccaatcta cgctacggtc gaagacatct ggatcatcgc cgtccatgcc
     5521 aaagacaaaa ctggcgttga ccatcacacc caggtcatgc agtcggcgga tggcggcttc
     5581 gtacccaggg agttgatccc tacgggttag gttgtggtat ttgtgctgag tacgcaggtt
     5641 atcga
//
LOCUS       DBNGSX010000414         4004 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_245284, whole genome shotgun sequence.
ACCESSION   DBNGSX010000414 DBNGSX010000000
VERSION     DBNGSX010000414.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_245284"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="AC16CB_12210"
     CDS             complement(<1..187)
                     /locus_tag="AC16CB_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009810101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIR3343279.1"
                     /translation="MASKDDRNPGMPLDLDWVAGAHVNRSAVERRVATLPARRSIKKD
                     WQAAWLLRAITCIDLTTL"
     gene            505..1497
                     /locus_tag="AC16CB_12215"
     CDS             505..1497
                     /locus_tag="AC16CB_12215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HIR3343280.1"
                     /translation="MIELKELQKVIGENTVLDIPSLAIQDGEIAALVGPAGSGKDVLL
                     ILLLGQTRPSMGTVRLAGVDPFHEKSQLSRHVGVLFAEDSVYKNLTSLANLEFQCKLY
                     GLPKSRATEILDQVGLADHSRTKLEKMSTSLLRRLAFARAILHSPQVLLLEEPFARCD
                     EATISLLSNMIRQQASLERAVLLIATDVSPYESFCDSIYLLNKGRIVESRHPDEERQA
                     NMPFKIPVRLDEKVVLFNPADILYADAAEGRAFIVTPDGRLPTQYTLSELEQRLVRSG
                     FFRAHRSYLVNLQHVKEVIPYTRNSFTLRLNNKENTEIPLSKSAAGELKDLLDY"
     gene            1678..2418
                     /locus_tag="AC16CB_12220"
     CDS             1678..2418
                     /locus_tag="AC16CB_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3343281.1"
                     /translation="MKAIEVQNLTRDFNDLRALDHISFSVEQGEIFGFLGPNGAGKTT
                     TIKILTGQLRPTAGQAWVLGCDVVTERHLLKPQIGVVFENQNIYERLSARDNLVFYSR
                     LYGIGRERVESVLAQIGLDGRADQRVAKYSNGMKQRLLIARALLHNPKVLFLDEPTRG
                     LDPNLAREIRAFILNQSRQGVTVFLTTHYMEEADQLCNRVAILDQGRIAALDTPERLK
                     VKYRKDNQATLEDIFVHLTGRSLQQEMV"
     gene            2431..>4004
                     /locus_tag="AC16CB_12225"
     CDS             2431..>4004
                     /locus_tag="AC16CB_12225"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3343282.1"
                     /translation="MKNTIPKGDGKQRIMENTRLIWAITAKDILAALKNKNTISVIIS
                     ALFVVIAYRVLPTLTNLDSSPNVLIYDGDESALVAFMQNSDVLEVHTFPTEEKMKSAL
                     SNADVPELGLVLPAGLDQALEAGQAPELQGYLMYWVDEADAIELKNRVEAEITRLVGT
                     PVDIQVAGNVVYNSTDSSGLGNWAALATVFVVATIGLSMIPHLMLEEKQSHTMEALLV
                     SPANAYHVVAAKALVGLFYCLLIASIALGLNHNLVLHWELAIAAAVLGSLFTISLGVL
                     LGTLIENRGQLTLGASILIAPLFFPIILSLMDDLFPAVLIQVFRGVPTTVQFNLLRAS
                     FAGSFPLGKTLLQLVWLVVCAAPGLLATAWLVHRQDRGVPVLSSLRQRYFPKSAPIKD
                     LLAPISAILSRIRQPQESPKAPLQKIATTQVQIPAAKSGFAGSLRVVWAIVAKDFLEA
                     VQNKIVLSIIIGTSFMVASSAIIPLILRSRDIPSAVVYDQGRSTIVRALTGRDDFRIR
                     IVTKQEEMEELITQSP"
BASE COUNT          995 a         1045 c         1028 g          936 t
ORIGIN      
        1 cgagcgtggt gagatcgatg caagtgatgg cgcgcaacaa ccaggctgct tgccagtctt
       61 tcttgatcga ccgccgagcc gggagggtag caacacgtcg ctccactgcg ctacgattga
      121 cgtgtgcgcc cgccacccaa tccagatcga gcggcatacc gggattgcga tcatctttgg
      181 aagccatcgt tcaatccttt ttgatccttt gcgtaaatat tgcctttaga aattgcgcaa
      241 tcagagcttc tttgggtcaa ctgcgcaagt tattgttttt taattgtacc aaaataactt
      301 gcagcatgcg gttatgcaaa gcagggcaat tgcataatcc ctgccaggca tgtgaaatcc
      361 tgcaaaacgt ggggtggggg gaaatcgctc caatgcgatt ggcttaatac atagatcaac
      421 ccaagtgatc ccacaggaaa gatgctaaaa taaatgagac catgtcgttg atatagctct
      481 actgaataac gctgaagaat aaacgtgatt gagcttaagg aacttcaaaa agtaatcggt
      541 gagaacaccg tactggatat tcccagtctt gccatacaag atggcgagat cgctgccctg
      601 gtcggcccgg cgggcagcgg gaaagacgtc ctccttatcc tgctcttggg gcaaacccgc
      661 ccaagtatgg gtaccgtccg gctggctggg gtcgacccat tccacgaaaa gagccaattg
      721 agtcgccatg ttggtgtgct gtttgccgag gactcggtgt ataagaatct cacatcactg
      781 gcaaaccttg aattccaatg caaattgtac ggattgccca aatcgcgcgc aaccgagatt
      841 ttagaccaag ttggcctggc cgatcactcc cgcaccaaat tggaaaaaat gtcaaccagc
      901 ttgctgcggc gactggcatt cgcgcgcgcc atcttacaca gcccacaagt attactgcta
      961 gaggaacctt tcgccaggtg cgatgaggct accatttcat tgctcagcaa catgatccgc
     1021 cagcaagcca gccttgagcg ggcggtgctc ttgatcgcca ccgatgtatc accgtatgaa
     1081 tctttctgcg attcgattta tctactgaac aagggacgca ttgttgagag ccgccaccca
     1141 gacgaagaac gacaagcgaa tatgccgttc aaaatccccg tccgcctgga tgaaaaagtg
     1201 gtgctattca acccggctga catcttatat gccgatgctg cggaggggcg ggcttttatc
     1261 gtaacacctg acgggcgttt gccgacccaa tacaccctat ccgagctaga acagcgcctg
     1321 gtgcgcagcg gtttcttccg cgcccaccgc agctacctgg tcaacctgca gcacgtaaaa
     1381 gaagtgatcc cctatacccg taatagcttc accctgcgcc tgaacaacaa agaaaacaca
     1441 gaaatcccgc tcagcaagtc ggctgcgggt gagttaaaag accttttgga ttactaatgg
     1501 gggctaacat cccgctccaa tcaacctttg ccagacacca ttcactagac gaaatgcgcc
     1561 tttgggcgct attttgttgc gcccggctct tcatttatat atactgggat aaagcgaaat
     1621 tatcgaatgc aggtttgaga tataaccatc atggagagca attacgggag tattggaatg
     1681 aaagcgatcg aagttcaaaa tctaacccgc gactttaatg acttgcgggc attagatcac
     1741 atcagcttca gcgttgaaca aggtgaaata tttggattct tgggccctaa cggtgcaggg
     1801 aaaaccacga cgatcaaaat cctgactggt cagctaaggc cgactgctgg tcaagcatgg
     1861 gttctgggct gtgatgtagt cacagagcgc cacctgctta agcctcaaat tggcgtggta
     1921 tttgagaatc aaaacatcta cgaaaggctg tctgcccgcg ataacctggt cttttatagc
     1981 cgcctgtacg gcattggcag ggagcgtgtc gaaagcgtgc tggcacagat cggcttggat
     2041 ggacgagcag accagcgggt tgcgaaatac tcaaacggca tgaaacagcg cttgctgata
     2101 gcccgcgctt tactacacaa ccccaaagtg ctgttcctgg acgaaccaac ccgcgggctt
     2161 gaccccaacc tagcccgaga aatccgtgcg tttattctaa accagagccg gcaaggggtg
     2221 acggtctttc tgaccaccca ttatatggaa gaggctgacc agctatgcaa tcgggttgcg
     2281 atcttagacc agggtcgcat cgccgctctg gatacacctg aacggctaaa ggtcaaatat
     2341 cgaaaagata atcaggctac cctggaagat atattcgtgc acctgaccgg caggtctctg
     2401 cagcaagaaa tggtttaggc aggagaagca atgaaaaaca ctatccctaa aggtgatgga
     2461 aagcagcgca tcatggaaaa cacccgtttg atctgggcca tcaccgccaa agatattctc
     2521 gcagccttaa aaaacaaaaa cacgatttcc gtgatcatat cggcactttt tgtcgtgatt
     2581 gcgtatcgcg tcttgcctac gttaacaaat ctagacagct caccgaatgt gctcatctac
     2641 gatggcgatg aatcagccct ggtcgctttc atgcaaaaca gtgatgtcct tgaggtgcat
     2701 acctttccaa ccgaggaaaa gatgaagagt gcgctttcga atgcagatgt gccagaactt
     2761 ggcctggtgc tgcccgccgg ccttgaccag gctctagaag caggccaagc gccagaattg
     2821 cagggctatt tgatgtactg ggtggatgaa gcagatgcca tcgaattgaa gaatcgtgta
     2881 gaagcagaga taacccggct ggtggggact ccggtagata tccaggttgc tgggaacgtg
     2941 gtttacaaca gcaccgattc cagtggtttg ggaaactggg ctgccctggc aacggtattt
     3001 gttgtcgcca cgattggtct atcgatgatc ccacacctaa tgctggagga gaagcaatct
     3061 cacaccatgg aagcactgct ggtttcccca gccaacgcgt atcatgtggt ggcggctaag
     3121 gcgctggttg gattgttcta ctgcttgctc attgcaagca ttgcgctggg cctgaaccac
     3181 aacctggtgc tgcattggga gcttgccatc gcagcggcgg tgctcggctc gctgttcacg
     3241 atttcgcttg gggttctgct gggaacgcta atcgaaaatc gcggccagct taccctagga
     3301 gcctcgattc tgatcgcccc gctgttcttc ccgatcatct tgtccttgat ggacgacctg
     3361 ttccccgcgg ttctcatcca ggtctttcgg ggcgtaccga caactgtgca atttaatcta
     3421 ttgcgtgctt ccttcgcggg cagtttccct ttgggcaaaa cattgctcca gttagtctgg
     3481 ttagtggtat gtgccgcccc gggtctgctg gcgacagcct ggttggtgca ccgacaagat
     3541 cggggtgttc ctgttttgtc tagcctgcgc cagcgttact ttcccaaatc ggcccctata
     3601 aaggacctgc ttgcaccgat cagcgccatt ctatcccgaa ttcgccaacc gcaagagagc
     3661 ccgaaagctc cactgcagaa gatcgccaca actcaggtgc aaatccctgc cgcaaaatcc
     3721 ggtttcgctg ggagcctgcg cgttgtctgg gcgattgtgg caaaagactt tcttgaagcc
     3781 gtgcaaaata aaatcgtact gtcgatcatt ataggcacca gcttcatggt agcctccagc
     3841 gcgataatcc cccttattct acgctctagg gatatccctt cggcggttgt ttatgaccag
     3901 ggtcgctcaa caattgtacg ggcgctcacc ggtcgggatg atttccgaat acggattgtc
     3961 acaaaacaag aggagatgga ggaattgatc acgcaatctc ctga
//
LOCUS       DBNGSX010000415         4022 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_800084, whole genome shotgun sequence.
ACCESSION   DBNGSX010000415 DBNGSX010000000
VERSION     DBNGSX010000415.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_800084"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="AC16CB_12230"
     CDS             complement(<1..863)
                     /locus_tag="AC16CB_12230"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR3343283.1"
                     /translation="MSVITMILVFGGGAAIVLLLVGLVITMTSERSLVEERLGRYIHE
                     EEAESSEKRERTSVLGDWLNVRLEKSSWGDRVARELARADVKLKTGEYLALMIIVGLI
                     SGFIAWYIGGRYILFGTLGGVLGTFIPRLYVKREQGRRLVRFGDQLADMLNLMVNGLR
                     AGYSTMQAMEAVSRELPPPISEEFRRVVQEMQLGISAQNALYNLLRRIPSDDLDLVVA
                     AMNVQREVGGNLAEILDTISHTIRERVRIKGEIRVLIAQVMYSARFLALLPVILMFAL
                     WLLNRPYMMEF"
     gene            complement(889..2289)
                     /locus_tag="AC16CB_12235"
     CDS             complement(889..2289)
                     /locus_tag="AC16CB_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TadA family conjugal transfer-associated ATPase"
                     /protein_id="HIR3343284.1"
                     /translation="MSLLKRIEQGSSTSQTNGSEGNTPSRLTNLQARRVAPPGVSAQR
                     DTYMDLKTRVQNRLLAELDPSMDISKVLEVRTTIQELFEQVLSEENIVLSRPERHRLF
                     EQIAAEILGFGPLQPLLEDEAITEVMVNGAKNIYVEKSGKIFRVPFSFESDDHVMRII
                     DRIVAPLGRRIDESSPYVDARLPDGSRVNAVIPPISLVGPVITIRKFYKNPITIEQLI
                     EFATLTPESLQFLKACVEARLNIVISGGTGSGKTTLLNILSQFIPNDERIITIENAAE
                     LQLRQEHVVTLESRPPNIEAKGEVTIRQLVINSLRMRPDRIIVGEIRDEAALDMLQAM
                     NTGHDGSMTTAHSNSPRDTLARIETMALMAGMDLPVRAIREQVASAINLIVHQDRMRD
                     GSRKVVNITEVSGMEGDIITMTDIFLFEQSGYEDGKVIGRLRPTGLRPKFIDQIEAAG
                     IHLPASIFGIGERRRY"
     gene            complement(2313..3578)
                     /locus_tag="AC16CB_12240"
     CDS             complement(2313..3578)
                     /locus_tag="AC16CB_12240"
                     /inference="COORDINATES: protein motif:HMM:NF022077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpaE/TadZ family protein"
                     /protein_id="HIR3343285.1"
                     /translation="MVPDEKVRVVVVDDIAETRENIRKLLQFENDFEVIGVARTGREG
                     IEIVKETKPDVVIMDINMPDMDGITATETIRRNLPYTQIVILSVQGDPNYMRRAMLAG
                     ARDFLTKPPPVDDLISAIRRAGKMAQDERAKTPSRAALGATGSLSSSSSLITGGKVIA
                     VYSPKGGTGCTTVAVNLAMALHNDETPVIIVDGNLQFGDIAVFLNEQGRNSIADLAPR
                     VDELDPDIIEEVAITHANSGVKILSSPSRPENAEGITGEQFLKILNYLRRLFSYIVVD
                     TSSILTDPTLAAIDASDLVILMTNQDIPSIKNSRLFLDLAEILKINRKRIVFIMNRFD
                     RRIGITPEKVGESFKHPISAVLPLDERVVIPSINRGRPFILDDKSRPISRAVLSLAEV
                     VRQRLSELTEAESQGEKVVGMNMGRLAKR"
     gene            complement(3606..>4022)
                     /locus_tag="AC16CB_12245"
     CDS             complement(3606..>4022)
                     /locus_tag="AC16CB_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343286.1"
                     /translation="QDAIVLQIGSFPYKGQEENEPQGEGQGQAEQPPEEEQQQQREGE
                     QPPAPPPPDVITLIVSPQDAITLNYLMYSGAQLTLALRAAQDDTRVQTESVTLQFLLE
                     QYSISVPAKLPNSMEPPVRELITPELSNDSIPTPAP"
BASE COUNT         1062 a         1021 c          870 g         1069 t
ORIGIN      
        1 aaaaattcca tcatataagg tctattaaga agccacagcg caaacatgag gataactggc
       61 aaaagtgcca aaaagcgtgc tgaatacatg acttgggcaa tcagcacccg aatctcaccc
      121 ttaattcgca ctctttcccg gatggtgtga gagatggtat ccaaaatttc agccagatta
      181 ccacctactt ctctttgcac attcattgca gctacgacca gatcgaggtc atcgctcggg
      241 attctgcgca ggagattata caaggcgttc tgagcagaga tacccaattg catctcctgg
      301 acaacccggc gaaattcttc cgagatcggt gggggaagtt cccgactaac agcctccatg
      361 gcttgcatgg ttgagtatcc agcgcgcaaa ccattgacca tcaaatttag catatcagca
      421 agttgatcgc caaaacgcac caaacggcga ccttgctcgc gtttcacata gagccgagga
      481 ataaatgtcc ccaaaactcc tccaagcgtt ccaaaaagga tgtatctccc gccgatatac
      541 caagcaataa atcctgaaat caaccccact atgatcatca gcgccaaata ttcgccggtt
      601 ttgagcttaa catccgcacg agctaattct cgagcaacgc ggtcacccca gctggacttt
      661 tccagtcgta catttaacca gtctccaagt acactagtcc tttcccgctt ttcagatgat
      721 tcagcttctt cctcgtggat ataacgcccg agccgctcct caaccagtga acgttcgctg
      781 gtcatggtaa taactaagcc aacgagaagc agcacaatcg ctgcaccacc tccaaaaaca
      841 agaatcattg ttattaccga cataatcact cacctgtgcg catggctact aataccttct
      901 ccgttcccca atgccaaata tcgatgctgg aaggtgaatt ccagctgcct caatttgatc
      961 aatgaacttc ggacgtaggc ctgttgggcg aaggcgacca atcaccttac catcttcata
     1021 tccgctctgc tcaaatagga agatatccgt catggtgatg atgtctccct ccataccgct
     1081 tacctcggta atatttacaa ccttccgact accatcacgc atgcggtcct ggtggacgat
     1141 taaattaatg gctgaagcca cttgttcccg aatagcacgc actggcagat ccatgccagc
     1201 cattaaggcc attgtctcga tacgtgccag ggtatctcga ggactatttg agtgggctgt
     1261 agtcatagaa ccatcatggc cagtattcat agcttgaagc atatctaagg cggcttcatc
     1321 gcgaatctca ccaacaataa ttcggtccgg gcgcattcgt aaagaattta ttaccaactg
     1381 tcgaatagtt acctcgcctt tcgcttcaat attagggggt cttgactcta gcgtaaccac
     1441 atgttcctgg cgtaattgga gttcggcggc gttttcaatg gtaataatgc gctcatcgtt
     1501 gggtatgaac tgggatagaa tattgagcag agtagtttta cctgaaccag tacctcctga
     1561 tatcacaatg ttcaacctgg cttcaacgca cgccttgaga aattgaagtg actctggagt
     1621 aagagttgca aattcgatga gctgttctat ggtgattggg ttcttataga atttccgaat
     1681 ggtgataaca ggacctacga gcgagatggg tgggatgaca gcattaacac gcgatccgtc
     1741 tggcaggcga gcatccacgt atggacttga ctcatcaatg cgtctaccga gaggggcgac
     1801 gatgcggtca ataatacgca tcacatgatc atcgctctcg aaactaaatg gaactcgaaa
     1861 aatcttccca gacttttcaa cgtagatatt cttagctcca ttgaccatta cttccgtgat
     1921 cgcttcatct tccaacaacg gctgtaatgg accaaatcca agtatctcgg cagcgatttg
     1981 ttcgaacaga cggtgccgct cgggacgaga caagacaatg ttttcttctg aaagcacttg
     2041 ctcgaagagt tcctgaattg tggtccgtac ctccagaacc ttagatatat ccatcgaagg
     2101 atcaagctca gccaacagcc tgttctgcac acgcgtcttt aaatccatat atgtatcgcg
     2161 ttgtgcgctg acacctgggg gagcgacgcg tcgtgcctga agatttgtaa ggcgtgaagg
     2221 cgtgttgcct tctgagccat tcgtctgcga tgtagaactt ccttgttcaa tccgcttcaa
     2281 tagcgacata aaataccact cctataccaa gcttatcttt ttgcgagccg ccccatattc
     2341 ataccaacaa ccttttcacc ctggctctcg gcttcggtaa gttcagataa tctttgtcgt
     2401 actacttctg caagtgatag cacagctctt gagattggac gcgacttatc atctagtatg
     2461 aatgggcgtc cgcggtttat ggaagggatt accacgcgct catctagagg aagcactgca
     2521 gaaataggat gcttgaaact ttctccaacc ttctcgggtg taatgccaat ccggcggtcg
     2581 aagcgattca ttataaacac aatgcgcttt cgattgatct taagaatttc tgctaagtct
     2641 aagaataacc tagaattctt aatagagggg atatcttgat tggtcattaa tatcactaaa
     2701 tcactcgcat caatcgcggc aagtgtggga tcggtgagta tagacgatgt atcgactacg
     2761 atataagaaa acaatcgcct gagatagttt aagattttta aaaattgctc tccggtaata
     2821 ccctctgcat tctctggtct agatggagac gataagattt taacccccga attagcatga
     2881 gtaatcgcaa cttcttcaat aatgtctgga tcgagttcat caactctagg cgcgagatcg
     2941 gcgatactat tcctgccttg ctcattgaga aaaaccgcta tatcgccaaa ctgcaggttt
     3001 ccatcgacaa taatcactgg cgtctcatca ttgtgcaacg ccatggctaa atttacagca
     3061 accgttgtgc aaccagtacc tccttttggg ctatatacag caatcacctt cccgccagtt
     3121 attaaagagg atgaggagga taacgaacca gtagcaccaa gagctgcccg agagggggtc
     3181 tttgcccgtt catcctgtgc catcttccct gcccgccgta tggctgagat taaatcatct
     3241 actggcgggg gtttggtaag aaaatctcgt gcccccgcca gcattgcacg gcgcatataa
     3301 ttgggatctc cctgaaccga taagatgacg atttgggtgt aggggagatt tcgccggatt
     3361 gtctcagtag ccgtaatacc atccatatcc ggcatgttaa tatccataat tacgacgtca
     3421 ggtttcgtct ccttgacaat ttctatccct tctcgtccgg ttcttgcaac accaatgacc
     3481 tcaaaatcat tttcaaattg tagcaactta cggatattct cgcgcgtttc cgctatgtca
     3541 tcaactacta cgacgcgaac tttttcatct ggaaccatcc aataaactcc gattgacagg
     3601 ttgaattaag gagcaggggt aggaatacta tcattcgata actcaggcgt tatcaattcc
     3661 ctcacaggag gctccatgct attcggtaat ttagccggca cagagatgct gtactgctct
     3721 aaaaggaact gtaacgtaac tgactcggtc tgcacacgtg tatcatcctg tgcggctctc
     3781 aaagccaatg tcaattgagc accgctgtac atcaaatagt tcaatgtaat cgcgtcttga
     3841 ggtgacacga ttaatgtgat gacatcgggc ggtggaggcg caggcggttg ctccccttct
     3901 ctctgctgct gttgttcttc ttcaggtggt tgctccgcct gaccttgacc ttccccttgg
     3961 ggctcatttt cttcctgtcc cttataaggg aagctaccga tctgcagaac aatcgcgtct
     4021 tg
//
LOCUS       DBNGSX010000416         7587 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1769858, whole genome shotgun sequence.
ACCESSION   DBNGSX010000416 DBNGSX010000000
VERSION     DBNGSX010000416.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7587)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7587)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7587
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1769858"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="AC16CB_12250"
     CDS             <1..762
                     /locus_tag="AC16CB_12250"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR3343287.1"
                     /translation="DDPPPLLSTTTVNRQAPTSLPSRKGTTIRSNDLPVETADPTQPQ
                     SAPIAPDTEVPAPAFSVCSPLVDTPLEELSQIVSEPYRPPPPNSEARHHGVDFAYFRR
                     YDRAAIEGAGIQSVLNGQVAASIVDSFPYGNMLILETPGSLLPPVLADRISMGAGESL
                     YTLYAHMERPPALRLGDSVRACQPLGNVGKSGNAGGSHLHLETRLGPAGTRFSGMAYY
                     KVWATDEQRENYLLWRTSGTYRHFDPLTLWTSDPK"
     gene            complement(799..1911)
                     /locus_tag="AC16CB_12255"
     CDS             complement(799..1911)
                     /locus_tag="AC16CB_12255"
                     /inference="COORDINATES: protein motif:HMM:NF017072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-fold NAD(P)-binding domain-containing
                     protein"
                     /protein_id="HIR3343288.1"
                     /translation="MMKEPGEKRVFLPEFIQYLSESGLIIFIEESYGSRSGWSFDDYK
                     QGNENVRSCSQLEAFQQDIVLVLRSPTFEEFQLLRPGTNLISMLHFPTRPKRVEALKE
                     LGVDAISLDSIVDDNNIRLVENMRAVAWNGLEAAFDVLEKRWPGLRRQDDQPIRVLIL
                     GTGMVGKHASEAATKLGNIERNNQHIEDGGPGSVAISIGRNMTHHPERLKKLFNEADI
                     LVDATQRRDTSVPVVPNAWIEWLPQHAVITDLAVDPYTLDTQPPVVRGIQGIPKGNLD
                     QYVFYPDDPNWEKTVPTTVPNQHRRTVVSCYSWPGIHPEACMRHYALQLDPLMEVLIT
                     KGYDGLDLHGGYFERAMYRGTLRTWLNSGHYEPRPR"
     gene            complement(1992..3170)
                     /locus_tag="AC16CB_12260"
     CDS             complement(1992..3170)
                     /locus_tag="AC16CB_12260"
                     /inference="COORDINATES: protein motif:HMM:NF017072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-fold NAD(P)-binding domain-containing
                     protein"
                     /protein_id="HIR3343289.1"
                     /translation="MQINIGLARMHMEPGERRDFLPGFVARLEHYGARVVLEYGYGSG
                     MTLVEEDYLQVAPSVTFATRQEVYQQDIVLVLRCPDTNDISMLRPGACFISMLHYPTR
                     PQRVEFLRSLGIEAVSLDSIKDDSGRRVVENLRSVAWNGIEAAFKVLRNTYPDPGFGS
                     KQRSPIQVTLMGTGAVGSHVVSAASRYGNENLRAKMVAAGIPGVKVVAIDYDVTPHEE
                     IMREILMNTDILVDATQRPDPSKPVIPNQWVGYLPQHAVILDLSVDPYNCDTQLLSIK
                     GIEGIPQGNLDRYIFSPDDPEFDILPACISTIHRRHTVSCYSWPGIYPKKCMELYGRQ
                     IRPILHVLIDKGGPQNIDPEGSFFERAIARALLSRLPIDNNAVQSTQYSQQRNNKDGS
                     "
     gene            3347..3916
                     /locus_tag="AC16CB_12265"
     CDS             3347..3916
                     /locus_tag="AC16CB_12265"
                     /inference="COORDINATES: protein motif:HMM:NF036525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3343290.1"
                     /translation="MTRDEILEAAAQIFREKGFHAASMQDIALAVNLQKASLYHHVSS
                     KQEILLALLDRALDLLIEHIEQVISQPLSPDEKLRQAMRVYLQVMLENRDLAAVLLLE
                     HRSLEPQYHARHTPRRDRFERLWRDLINDGHEQGIFSCADPALASRFVLGVLNWSITW
                     YRPDGSLSPTEIADQFAEMFLKGLLVRKS"
     gene            3916..4986
                     /locus_tag="AC16CB_12270"
     CDS             3916..4986
                     /locus_tag="AC16CB_12270"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3343291.1"
                     /translation="MMVEEPLSKPPEVSLFETSRGARIFQIPLQEFPILWGYAYLVLL
                     DDPQEGSYRVLIDSGSGFGDSNQHLEYGFQRISQIIGQTIGLEHLTHVFITHGHIDHF
                     GGLTYVRPRTQARIGVHELDLRNLTNYEERLTIVARRLEAFLFEAGVSPGRHANLIEM
                     YMLPKSLFHSIHVDFTYEAAGMRVGPFEFLHVPGHCAGHVVIRLHDVLFSGDHVLDGL
                     SPHQSPEHLTLFTGLDHYLKSLDALEAWAGDARITLGGHRGVITDLPGRLNAIRQVHT
                     KRLNSVLEILEQPHTISEVSHALFGDVHGYNVLLALEETGAHVEYLAQRGRLRISNLD
                     EIEKNHNPVAIRYQKLALNGEE"
     gene            5041..6135
                     /locus_tag="AC16CB_12275"
     CDS             5041..6135
                     /locus_tag="AC16CB_12275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3343292.1"
                     /translation="MFSFEPTEEQKMLVDAIGRYAQSDLRAKAHDADEEDQYPQSLIE
                     KGWELGFLQASIPERYGGFGERSVVTGALAAETMAWGDLAGALAVMTPGLFAMPILMA
                     GSEEQKQEYLPRVIEAEWQPYTAALIEPHYDFDPNELRCVAAQEDGSYVISGEKAYVP
                     YAISADTMIVYARLGDITQGFIVPVGTDGLELGEREKLMGVNALPLYRLSLNQVRIPL
                     ENRLGGPQGHDFAPILSASRVALAAMAVGLSRAALEYACDYAKDRDVFGVKVAQKQVI
                     AFMIAEMATEVEAIKLLNWEAAWMLDNNKEDAAKNAYLAHTGAIDMAMMVTDRAVQIL
                     GGHGYIREHPVELWMRNGRGIAMLTGLAIV"
     gene            6163..7413
                     /locus_tag="AC16CB_12280"
     CDS             6163..7413
                     /locus_tag="AC16CB_12280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3343293.1"
                     /translation="MIDFETPKPIAQQSVALKTVAENMMRPVSRYFDEHEHEIPWDYI
                     NFMHMAMRATGAGSLTPRERKKNGDEPKKDRPPIGYQSLAHLLEIWAGGDVGIYWVTP
                     GGGLGAAAVEATGTPEQKEKFLARFKEERPTFAAMAMTEPQAGSDTSAIRSTAVLDKS
                     TNEWVLNGEKIFVTAGHKSLCESNGFVVIWATIDPKAGRAGMRPFVVEASAPGVQVTK
                     LEHKMGIRASDTASIVMQDCRVPFENILGSPEVEVETTKGFKGAMATFDATRPLVAAT
                     GVGVGRATLELLKELLAQHGVTIRYGLPRQKLTSIERDVIDMEVMLRSAWLLVIKAVW
                     MADNRKHNPLEASMSKLRAGDVVNKITQKAVEIMGPLGYSRDYLLEKWFRDAKITDIY
                     EGTGQINRLIVARNVLDYSGRQLR"
BASE COUNT         1749 a         1907 c         2150 g         1781 t
ORIGIN      
        1 gatgatcctc cgccactgct ctccacgact actgttaatc gacaagcgcc caccagcctg
       61 ccctctcgca agggcactac catacgatcg aatgaccttc cggtagagac tgcagatccg
      121 acccaacccc aaagcgctcc tatagcaccc gatacagaag taccagcgcc ggctttttcg
      181 gtttgctcac cgctggtaga tactcccctg gaagaattat ctcagatcgt atcggaacct
      241 taccgacccc cacccccgaa tagcgaggcg cgccatcacg gtgtggattt cgcatatttc
      301 cgccgctacg accgggcagc gattgaggga gctgggattc aatctgtgtt gaatggacag
      361 gtggctgcat cgattgtgga tagcttccca tatgggaata tgctcatttt ggagacgcct
      421 ggctcgctgc tgccgccagt tctggcggat cggatctcaa tgggtgctgg agagtcgctt
      481 tacacactct atgcccatat ggagaggcca cctgcgctga ggttggggga tagcgtacgc
      541 gcctgccagc cgctgggtaa tgtgggtaag agcggcaatg ctggcggctc tcacctacac
      601 ctggaaactc gtctcggacc agccgggacg cgtttctctg gtatggctta ttacaaggtt
      661 tgggcaacgg atgagcagcg ggaaaattat ctcttatggc gcactagcgg gacatatcgc
      721 cattttgacc ccttgaccct ttggacgagt gatccaaaat aaaagacgag cctttacggt
      781 gatagtgcgt actaagcctt agcgtgggcg aggttcgtag tgcccgctat tcagccatgt
      841 tcttagggtg ccacgataca tcgcgcgctc aaaataacca ccgtgaaggt caaggccgtc
      901 ataccctttt gtaatcagga cttccattag gggatcaagt tgtagggcat agtgacgcat
      961 gcaggcttct ggatgaatgc ctggccagga gtaacaggaa accaccgtac gccggtgttg
     1021 gttggggaca gtggtgggca ctgtcttctc ccagttaggg tcgtcgggat agaagacata
     1081 ctggtccaag tttccctttg ggataccctg gataccgcgg accactggag gttgggtgtc
     1141 tagcgtgtag gggtccactg ccagatctgt aatcacggcg tgttgtggca gccattctat
     1201 ccaggcgttt ggcactaccg gaacagaagt gtcgcggcgt tgggtggcat ccactaatat
     1261 atcggcttcg ttgaatagct tcttgagcct ctcagggtgg tgcgtcatat tgcgcccaat
     1321 ggagatcgca accgatccgg gtccaccatc ttcaatatgc tggttattgc gttcaatatt
     1381 gcccaatttc gtggcggctt cactggcatg cttgcctacc atgccagtgc ccaggattag
     1441 cacccggatt ggctggtcgt cctgtcgcct caatccaggc cagcgcttct ctaacacatc
     1501 gaaggctgcc tccaatccgt tccaagccac cgcacgcatg ttttcgacca ggcgaatgtt
     1561 gttgtcatcg acaatgctgt ccaatgaaat ggcgtctacc ccaagttctt tgagcgcttc
     1621 tacccgtttt ggacgagtgg gaaagtgcag catcgatatc agattagtgc cgggacgtag
     1681 cagctgaaac tcctcaaaag tcggagaacg cagcaccaga acgatgtctt gctggaaggc
     1741 ctccagttga gaacacgagc gcacgttctc attcccctgc ttgtaatcgt cgaaactcca
     1801 ccctgagcga gagccgtagc tctcttcgat aaaaataatt aaacctgatt cgctcaggta
     1861 ttggatgaac tcagggagga aaacccgttt ttcaccaggt tccttcatca tgcgagggaa
     1921 gccgactgat tttggaagag atatagttgc acctcgtaat aagatttgag aatgatgttg
     1981 gacctacctg gtcaggagcc atccttgttg ttgcgttgct gagagtattg tgtgctttgg
     2041 accgcgttgt tatctatagg taagcgagag agcaaagcgc gggcaatggc gcgttcgaaa
     2101 aagctgccct cgggatcgat attttgcggt ccccctttgt ctatcaggac atgcaaaatg
     2161 gggcggattt gccgtccgta caattccatg cacttctttg gataaatgcc aggccacgag
     2221 taacaagata ccgtgtggcg gcgatggatg gttgaaatgc aagccggcag gatgtcaaac
     2281 tctgggtcgt ctggagagaa gatatagcgg tctaggttgc cttgagggat accttcaata
     2341 cctttgatgg aaagcagctg cgtatcacag ttataaggat ctaccgagag gtcgagaatg
     2401 actgcatgtt gcggtaaata acccacccat tggttcggaa taacgggttt acttgggtcg
     2461 gggcgttggg tggcatccac taagatatcg gtattcatca ggatctcgcg catgatctct
     2521 tcgtggggcg tgacatcgta atcgatggcg accactttaa ctcccgggat tcccgcagct
     2581 accattttcg cgcgcaggtt ttcattgcca tagcggctag ctgctgaaac aacgtgactg
     2641 cctaccgcac ctgttcccat caatgtgacc tggatggggg agcgttgctt gctgccgaaa
     2701 ccgggatcgg gataggtgtt gcgtaatact ttgaaagcag cctcgatgcc gttccaggct
     2761 accgagcgca ggttctccac cactcgtcgc ccagaatcat ctttgattga atccagggac
     2821 acggcttcaa tacccaaaga gcgtaggaat tcaacccgct gaggacgtgt tggataatgt
     2881 agcatagaaa tgaagcatgc gcctggtcga agcatggaga tatcgttggt atcgggacag
     2941 cgcaggacca gtacgatgtc ttgttggtaa acctcctggc gggtagcaaa agtaacggac
     3001 ggagcgacct ggaggtagtc ctcttcgact agtgtcatgc cgctcccata gccatattcg
     3061 agcacaaccc tagcgccata atgctccagc ctggctacaa atccgggcaa gaaatcgcgt
     3121 cgctcgcctg gttccatatg catgcgcgcc aggccgatat tgatctgcat ttgcgtagtt
     3181 ctccttcgtt cctgtgataa cgaactcagt ttaccacagc ttaatatggc acaagtaacg
     3241 attggactat gccggaggca tacataccat gttgtaagtt gacattaggg ttggtttttg
     3301 atatactaaa tctaccgacc gttcggtagg gagtaaagga aaagttatga cccgggatga
     3361 gatcctggaa gctgccgctc aaatctttcg tgaaaaaggt ttccatgccg cctcgatgca
     3421 agatatcgcc ctggcagtaa acctgcagaa agccagcctt tatcaccacg tttcgtccaa
     3481 gcaagagata ttattggcgc tgctcgatcg ggcgttggac ctgctaattg agcatatcga
     3541 gcaggtgatc tcgcagccgc tctctcccga tgaaaagctc cgccaggcaa tgcgcgtata
     3601 tttacaggtc atgctggaaa atcgtgatct ggctgcagtt ttgctcctgg aacatcgctc
     3661 ccttgagcct cagtatcacg cccgtcacac accgcggaga gaccgttttg agcgcctgtg
     3721 gcgggatttg attaatgatg gtcacgagca gggcattttt tcctgcgcag accctgccct
     3781 ggcttcccgc tttgtgctgg gtgtcctgaa ttggtccatc acttggtacc gcccagatgg
     3841 ttccctttca cccactgaga tcgccgatca atttgccgaa atgtttttaa agggtttact
     3901 ggtccgtaaa agctgatgat ggttgaagaa ccgctatcca aaccccctga ggtaagtcta
     3961 tttgagactt cccgtggcgc gcgtatattt caaattcccc tccaggagtt tcccatcctg
     4021 tggggttatg cctatctggt tctactggat gacccccagg aaggctctta tagggttttg
     4081 attgatagtg gctcgggttt tggcgactcg aaccagcatc tggagtatgg attccagcga
     4141 atatcccaga ttatcgggca aacgattggc ttggaacacc tgacccatgt ttttatcacg
     4201 catgggcata ttgaccactt cgggggactc acctacgtcc gcccgcgcac gcaagccaga
     4261 atcggggtgc atgagcttga cctgcgcaac ttgaccaatt acgaagaacg tttgacaatc
     4321 gtcgctcgtc gcctggaggc attcttgttt gaagccggtg tttcgccggg gcggcacgcc
     4381 aacttgattg aaatgtacat gctgccgaag agcctgttcc attccatcca tgtggatttc
     4441 acgtacgaag cggcaggcat gcgcgtagga ccatttgagt ttttgcatgt acctggacat
     4501 tgcgcgggtc acgtggttat ccgcctgcac gacgtgctgt tcagcggcga ccatgtgcta
     4561 gatggcctta gcccacacca gtcgccggag catctaacgc tatttaccgg gttggatcat
     4621 tatctgaaat cactggacgc cctggaagcc tgggcgggcg acgcacgcat cacactggga
     4681 gggcaccgcg gggtgatcac agatctgcct ggccgcctta atgccattcg ccaggtgcac
     4741 acaaaacgat tgaattcggt gctggaaata ttggagcagc cgcacaccat ctctgaggtt
     4801 tctcatgcct tatttggcga tgtgcatggc tataacgtat tgcttgccct ggaagagacc
     4861 ggtgctcatg tggaatatct ggcccagcga ggccggctgc ggatcagcaa cctggatgaa
     4921 atcgagaaaa accacaaccc ggttgctatt cgctaccaaa aactagcatt aaatggggaa
     4981 gaatgacttg gatttgtcga gttaatctga tgagagagta gatccagaag gagtgtgacc
     5041 atgttctcat ttgaaccaac tgaagagcaa aaaatgctcg tggacgccat tgggcgttac
     5101 gcccaaagtg acttgcgcgc caaggctcat gatgccgatg aagaagacca gtacccgcaa
     5161 agcttgatag aaaaaggctg ggagcttggc ttcttgcagg catccatccc agagaggtat
     5221 ggtggcttcg gtgagcgttc tgtcgtcacg ggggctttgg cagccgagac aatggcgtgg
     5281 ggcgatctgg cgggggcgct ggcggtaatg acacccgggc tgtttgccat gccgatcctg
     5341 atggcaggct cggaagaaca aaagcaagaa tacttaccca gggtgatcga ggctgaatgg
     5401 caaccttata ctgctgccct gattgagcct cattatgact ttgatccaaa cgaactgcgc
     5461 tgtgtggcgg cgcaagaaga cggcagctat gtgatcagcg gcgagaaagc ttatgtgccg
     5521 tacgcgatta gcgcagatac aatgatcgtt tacgccaggc tcggagatat cacccagggc
     5581 tttatcgtcc cggtagggac tgatggcctg gaactaggcg agcgagaaaa gctgatgggc
     5641 gtcaatgcgc tgccattgta ccggcttagc ctgaaccaag tgcgcatccc gctcgaaaat
     5701 cgcctgggtg gcccgcaagg gcacgatttc gcacctatcc tgtcagcctc ccgagtagcc
     5761 ttggcggcga tggcggtggg cttgtctcgg gctgccctgg aatatgcctg cgattacgcc
     5821 aaggaccgcg atgtgtttgg cgtgaaggtg gcgcagaagc aggtgattgc gttcatgatc
     5881 gcagagatgg caacggaggt tgaagcaatc aagttgttga actgggaggc cgcctggatg
     5941 ctggataaca acaaggaaga cgctgctaag aacgcttacc tggctcatac cggcgccatt
     6001 gatatggcga tgatggttac cgaccgtgcc gttcaaattc tgggtgggca cggctacatc
     6061 cgagagcacc cagtggagtt gtggatgcgt aacgggcgtg gtattgccat gctcaccgga
     6121 ttggcgatag tgtaggggct ggatagatta cggaggttga aaatgatcga tttcgaaaca
     6181 ccgaaaccca ttgcacaaca gagtgttgca ttgaaaactg ttgcagaaaa catgatgcgc
     6241 ccggtatcgc gctatttcga cgagcacgaa catgagattc cctgggatta catcaacttc
     6301 atgcatatgg ctatgcgcgc tacgggcgcg ggctcgctga cgccgcgcga gaggaagaaa
     6361 aatggtgacg agccgaagaa ggatcgccca cctattggct accagtcgtt ggcgcatttg
     6421 ctcgagattt gggccggggg agatgttggc atatactggg tgacgccagg gggaggcctg
     6481 ggtgcagccg ctgtggaagc gaccgggact ccagagcaaa aagagaaatt tttagctcgc
     6541 ttcaaggagg agcgtccgac tttcgcagcc atggcgatga cagagccaca ggctggctcg
     6601 gatacttctg ccatccgcag tactgccgtg ctggacaaat ctactaacga gtgggtgctc
     6661 aacggggaaa aaatctttgt caccgctggg cacaagtcgt tgtgcgagag caatggcttc
     6721 gttgtgattt gggcaaccat cgatcccaag gctgggcgag ccggcatgcg cccctttgta
     6781 gtggaggcga gcgcacctgg ggtccaggtg accaagctgg aacacaaaat gggcatccga
     6841 gccagcgata cagcctcgat tgtgatgcag gattgccgcg tgcctttcga gaacatcctt
     6901 ggtagcccgg aggtggaggt ggaaaccacc aaaggcttta aaggcgctat ggcgactttt
     6961 gacgccactc gccccttagt ggcggcgaca ggtgttgggg ttggacgtgc gacgctagaa
     7021 ttgctaaagg agttgctcgc ccagcatggt gtgaccattc gctatggact accccggcag
     7081 aagctgacca gcatcgagcg cgatgtgatc gatatggagg tcatgctgcg ctcagcctgg
     7141 ctgttggtga ttaaggcggt gtggatggct gataatcgca aacataatcc tctagaagcc
     7201 tccatgagca agctgcgcgc gggtgatgtg gtcaataaaa ttacccagaa ggcagtagag
     7261 atcatgggac cactgggcta cagtcgggat tacctgctgg agaagtggtt ccgcgatgcc
     7321 aagattacag atatctacga gggcaccggg cagatcaacc gcttgatcgt agcccgcaat
     7381 gtattggatt acagtggcag acaattgcgc taaatgctag tgctcaggtc gggtaactgc
     7441 atgctcttac ccggcaaaca gcaccctaaa attcaaatat tgcttattgg tattggtaca
     7501 agatgaatga ccatcggaca aagcacctta acatcctagt tttgttgttg tctgccgtgt
     7561 taatcgcggg atgctcggcg cctgaaa
//
LOCUS       DBNGSX010000417         2223 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1769939, whole genome shotgun sequence.
ACCESSION   DBNGSX010000417 DBNGSX010000000
VERSION     DBNGSX010000417.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1769939"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="AC16CB_12285"
     CDS             complement(<1..236)
                     /locus_tag="AC16CB_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR3343294.1"
                     /translation="MRLKIYLLGQFRIEVDERAIELSSRPAQSLLSYLALNVGVTQRR
                     EKLADLLWPEASESNARSYLRQALWRIHKSLERGG"
     gene            complement(434..>2223)
                     /locus_tag="AC16CB_12290"
     CDS             complement(434..>2223)
                     /locus_tag="AC16CB_12290"
                     /inference="COORDINATES: protein motif:HMM:NF019341.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3343295.1"
                     /translation="GYWVEGSALDWERLPARVEAVIAERIGRLAEPLRAVLRVASVEG
                     EQFTAEVVARILGRDEKETLGRLSSELDRKHRLVRAQSIQRVDGQTLSCYRFRHILFQ
                     KYLYGSLDEVERVHLHEQVGTSLEGLYVAREEIVAIAVQLAMHYQKARIMERAIYYLH
                     LAGDKAVQLSAYQEGKAHLSRGLELLMSLPDSSQRDQQELALLISLGIAWKSDIPDPE
                     GEKALIRARELCQRMGKTTQLCRVLGELSVIPYVRAEYQKARELGEEALSLAEQAEDP
                     LLVALGHWHLGYVLFGLGEFTTSHAHLLQVISTYEPQQHHQPFVSLRGSDAGVSALAY
                     DACCLWCLGYPDQALSRSQEVLDLARDLGHPFSLADVLCFAGCLFNELRRDARALEQH
                     AEELERLSRKMSFLSFGETGTCYRGVALAMLGEIQEGIVLIRQGLAGRQSMEALCDFS
                     GIMSALAAAHAKGGQPEEGLATLDEALSLIEVTGERFLEAEHHRLRAELLLMQGDEEG
                     AEASLESAIEVARRQSAKSLELRATIDLARLWRERGRTDDARQVLGQIYNWFTEGFET
                     IDLIEAKALLEEISHETIQDMDDLQSMSG"
BASE COUNT          491 a          747 c          498 g          487 t
ORIGIN      
        1 ccccctcttt ccagcgattt gtggatacgc cacagcgcct gcctcaggta gctgcgcgca
       61 ttgctctcgc tcgcctccgg ccacagcagg tcggccagct tctcccgccg ctgcgtcacc
      121 cccacattca acgccagata cgacagcaac gactgcgccg gccgggatga caactcaatc
      181 gcccgctcgt cgacctctat tctgaactgc ccaagcaggt atattttcaa cctcatgccc
      241 tacttccaat ccgcagacaa actaccctat agcgccctac attcgcagcc tatgagataa
      301 attcctagtt cgcgacacac ttcggcgggc acataactgt aataatttta gcccagctat
      361 acaaggtggt ctaccttaaa gcagttaact aatagtcatg aatgacggtc tgattgcgat
      421 gggctccggc tttctaaccg ctcatcgatt ggagatcatc catgtcttga atggtctcat
      481 ggcttatctc ctcaagcaaa gccttagcct ctatcaggtc tatcgtctcg aagccttccg
      541 tgaaccagtt atagatctgc cccaacacct gtcgcgcatc atccgtcctg ccccgttctc
      601 gccacaggcg agccaggtca atcgtagccc gcagctccag cgacttagcg ctctgccggc
      661 gggcgacctc gatcgcactt tccaggctgg cttcggctcc ctcctcatcg ccctgcatga
      721 gcagcagctc tgcccgcaag cggtgatgct cagcctctaa aaaacgctcg cccgtcactt
      781 caatcagaga taatgcctcg tccaaggttg ccagcccttc ctctggttgc ccccccttgg
      841 cgtgcgcagc ggccagagca ctcattattc ctgaaaagtc acacagtgcc tccatagact
      901 gcctacctgc caagccctgg cgtattagta cgatcccttc ttggatctca cccagcatgg
      961 caagcgctac ccctcggtag caggttcccg tctccccaaa cgacaagaag gacatcttcc
     1021 tggataagcg ctcaagctcc tcagcatgct gctcgagcgc ccgcgcatcg cgacgcaatt
     1081 cattgaacag gcaacccgca aagcagagga catcggccag cgagaaggga tgccctaagt
     1141 ctcgcgccaa gtccagcacc tcttggctgc ggctcagggc ttgatcggga tatccaagac
     1201 accacaggca gcaggcatca tacgctagcg cgctcacacc cgcgtcagat ccgcgcagag
     1261 acacgaatgg ttggtgatgc tgttgaggct cataggtaga gatcacttgc aaaaggtggg
     1321 cgtgagatgt tgtgaattct ccgagaccga ataagacata tcccagatgc caatgaccca
     1381 gtgcaacaag caacgggtct tcagcctgct cggcaagact gagcgcctct tctccaagct
     1441 ctcgtgcctt ttgatactcg gctcgcacat agggtattac cgacagttca cccaataccc
     1501 ggcataattg agttgtttta cccatccgct ggcatagctc acgcgccctg atcaaagctt
     1561 tttccccctc gggatctgga atatctgact tccaggctat tcccaatgaa attaacaaag
     1621 ccagttcttg ttgatctcgc tgtgaagagt caggcaagct cataagcaac tccaagcccc
     1681 tgctcaggtg agctttcccc tcctgatagg cagacaattg cacagcctta tccccggcca
     1741 agtgcagata gtagatcgcc ctctccatta tccttgcttt ctgataatgc attgccaact
     1801 gcacggcgat agccacaatc tcctcccgag ctacatacag cccctccagg gaagtgccta
     1861 cttgctcgtg aaggtgaacc cgttccacct catcgagact gccgtacagg tatttctgaa
     1921 acaagatgtg ccggaagcga taacatgaca gggtctgccc atccacgcgt tggatactct
     1981 gcgcgcgaac caggcgatgc ttgcggtcca gctcgctgct caggcgcccc aatgtctctt
     2041 tctcatctct gcccaggatt cgggctacca cctcagcggt gaattgttct ccctctacac
     2101 tcgccacccg caacacagcc cgcagcggtt cagccaatcg ccctatacgc tccgcaatca
     2161 ccgcctccac ccgcgccggc agcctctccc agtccaacgc agatccctct acccagtacc
     2221 cct
//
LOCUS       DBNGSX010000418         1657 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1423498, whole genome shotgun sequence.
ACCESSION   DBNGSX010000418 DBNGSX010000000
VERSION     DBNGSX010000418.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1423498"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="AC16CB_12295"
     CDS             complement(<1..1001)
                     /locus_tag="AC16CB_12295"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3343296.1"
                     /translation="MSAKQEVFETRVKPLSTTKPAWWLIFVRELADLWIGGKALNLIL
                     IYSVILGIMVYVFSFNSELSLIPPKEAVYEMLKNAMAVSLFIALVIGADTISGERERA
                     TLESVLLTPTSRRQIVVGKFLAAFSAWPAAYLIAIPFMNVLSQGDEVLVPAIIWGAIT
                     GTALVMGYTGLGMLVSFWSGANKVSYFVSLGIYVLFLVPAQLPGRAQTGAAGQFLQWV
                     NPLAATNHFLSKHLVNYRTLAEFWTWLISPVVFSVLILVVLFLYAAPGLRLEAGKSSK
                     IWSFLTRVIGLFVIACLIFSLGSSPVSAASDLQIDIDMDYTVVKTGDKVEFNTLVTN"
     gene            complement(998..>1657)
                     /locus_tag="AC16CB_12300"
     CDS             complement(998..>1657)
                     /locus_tag="AC16CB_12300"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3343297.1"
                     /translation="YGVSTADARSNSLRLLEDVGLKQRAKSLIGTYSHGMRQRIGIAR
                     ALVNDPVVVFLDEPTLGLDPRGQQELLTLIKRIVQERNAGVILCSHALSEIEEICDDV
                     VILSSGQIIASGPVADVIGRAQRNVIRVRVPISSVDQATQKLEALPNIIKVASSNGKT
                     GWLVMERVGLTDTSSTPKPDANNKILEALIKAKIPVLGYEVEGGRLQDVFLHLTEEVI
                     K"
BASE COUNT          437 a          443 c          393 g          384 t
ORIGIN      
        1 ttgttggtta ccagcgtatt gaattcaact ttgtctcccg tcttcactac cgtgtaatcc
       61 atgtcgatat ctatctgaag atctgaggcc gctgaaaccg gcgatgaccc cagggagaag
      121 atgaggcagg cgatcacaaa aagacctatt acacgggtta ggaacgacca aatcttgctg
      181 gattttcccg cctcaaggcg tagacccgga gcggcatata ggaagagcac aactaggatt
      241 aggacggaaa acaccaccgg tgatataagc caggtccaaa attcagccag ggtgcgataa
      301 tttaccaggt gctttgaaag gaagtggttg gtggcagcca gcgggttcac ccattgcagg
      361 aattgccccg ctgctccggt ctgggcgcgc ccaggcaatt gtgccggaac gagaaaaagc
      421 acataaatgc ccaggctcac gaaatagctc accttgtttg cgcctgacca gaaactaacc
      481 agcataccca gacctgtata acccatcacc agcgcagtcc ctgtaatagc gccccaaata
      541 atagccggaa ccaggacctc gtcaccttgg gataacacat tcataaatgg gatggcaatc
      601 agataggctg ctggccatgc tgaaaaagca gctagaaatt tcccaacgac aatttgtcga
      661 cgactcgttg gtgtcaagag aaccgattcg agagtagcac gttcccgctc accgctaata
      721 gtgtcggcac caatcaccaa agcgatgaac aggctgacag ccatggcgtt tttcagcatc
      781 tcgtaaaccg cttccttggg agggatcaaa ctcagttcgc tgttgaatga gaatacatac
      841 accataatcc ccaggataac gctgtaaatg aggatgaggt tgagagcttt accgcctatc
      901 cacaggtcgg cgagttccct gacgaaaatc agccaccacg caggctttgt agtgctcaac
      961 ggtttgacac gagtttcaaa cacttcttgt ttagcgctca tttgattacc tcttcggtta
     1021 agtgtaggaa aacatcctgc aaccgaccgc cctcaacctc gtaccccaaa acaggaattt
     1081 ttgctttgat aagggcttct agtattttgt tattggcatc aggttttggg gtggaggatg
     1141 tatccgtcaa acccacgcgc tccatcacta accaacccgt cttaccgttt gaggaagcta
     1201 ctttgataat attcggcaat gcctccagtt tctgtgtagc ctggtcgacc gatgatatcg
     1261 ggacgcggac gcggatgaca ttgcgctgag cccttccaat gacatccgcc acaggtcccg
     1321 aggcaataat ctggcctgaa ctcaagatga ctacatcgtc acaaatctct tcaatctcgg
     1381 acaaagcatg gctgcacaaa atcacacccg catttcgctc ttgcacaatg cgcttgatga
     1441 gagtcaataa ctcctgttga ccgcgcgggt ccaatccgag agtgggttcg tctagaaaaa
     1501 cgaccacagg gtcattgacc agcgcgcgcg cgatgcctat cctttgtctc atcccgtgac
     1561 tgtatgtgcc aatcagggat tttgccctct gcttcaatcc cacatcttcg agaaggcgta
     1621 agctattgga tctggcatca gccgttgata ccccgta
//
LOCUS       DBNGSX010000419         5868 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1562104, whole genome shotgun sequence.
ACCESSION   DBNGSX010000419 DBNGSX010000000
VERSION     DBNGSX010000419.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5868)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5868
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1562104"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            434..712
                     /locus_tag="AC16CB_12305"
     CDS             434..712
                     /locus_tag="AC16CB_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343298.1"
                     /translation="MIGKTRSDGWGSLYPAEAMAVHPPLQAQALVLGAEDVDTAHQIR
                     EWLQDCGLRISARLRRTRTARQERKVAFSRSMKAVLSLVLPWLFFNSP"
     gene            1040..2734
                     /locus_tag="AC16CB_12310"
     CDS             1040..2734
                     /locus_tag="AC16CB_12310"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3343299.1"
                     /translation="MKGKTQTSSIPNRKQSSGILLTVLAIWHVLLALGCLAGAVVLWQ
                     RYTPEATWLQWLYIIVIVLIAASSAITVPLILRREHRGRILSLAINYLGFLICLFGSM
                     HALGIFVGIDSLASSFGRGILFLAGVLSGYLITTIGDRYENHPGRERFFRRLGKIIMA
                     IFGILFLFAVGILPGIISLLSRFNSPVTLALGVGVLLFGLMIWAMWQQPSAQAMGAKN
                     IHEEILNGYLFLSPNLFGFLFFFAGPLLLSFYVSFTDWDAFGSKNWIGLTNYAKIVNL
                     DIATLEDPKQRASEILDVKVYDELTRFSLFGKHIIIGAEDKLFWLALRNTFIFSVLAV
                     PLSIIPALFLANLLNSKLPGMKFFRAIYFLPSIAAVVGVALIWQWLYNAAVGYINYGI
                     TSMVGLINSVTDSALIDPQIRWLSDTRTALLAVVIMSAWQWIGFNTVLFLAGLQNIPG
                     ELYEAATVDGAGQWAKFWNITIPLLAPTTFFVVTTAIIQALQLFEQVYVLIPTEPPGG
                     PNNATLSLVLYLYQKGFQRFDQGYASAVAWVLFVVIFSVTLFQFLRQRSGRGAYSV"
     gene            2751..3965
                     /locus_tag="AC16CB_12315"
     CDS             2751..3965
                     /locus_tag="AC16CB_12315"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3343300.1"
                     /translation="MRSYHLGRLITNTLVYIILILFAFFMLFPFIYMFSTSLKYQKDT
                     FRYPPRLMPMQGITTQVEGFDEPLPLFYVDYNGEERKFALTESGIKIGIYAEPNNLEV
                     TYERPLTQVKPTGGVVNQAIVMVNGKEEKLFDVDVEGQMIPMIKVGQTALGNFIDPSD
                     PSIFVLQNVRLSQPVEEPAGRPENYKEVTELQGLDRSLTNTALVTILVVIGQLVTSVL
                     GGYALARLRFPGRDQIFVIYLGTIMIPFVVLITPLYQLMVMIGWVDKLAALIIPWIFT
                     AYGTFLMRQFFISYPKEIEESALIDGASRLQILVSILLPASVPALAALATFTFLYAWN
                     SFFWPLVIINTGNRANHVLTLSLSILRGRASDNANLVMAGAAIAILPPMIIYILAQRF
                     FVESATSSGVKG"
     gene            4107..5657
                     /locus_tag="AC16CB_12320"
     CDS             4107..5657
                     /locus_tag="AC16CB_12320"
                     /inference="COORDINATES: protein motif:HMM:NF046577.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6938 domain-containing protein"
                     /protein_id="HIR3343301.1"
                     /translation="MKIKDGRFVVYGNQISSSQSKKAIKWQNMFAKKFKYDPDETYTL
                     SLVDNEYLGPIFGLRDIVRSDQGEKIEPENAVICSTIRMGFGHYRIAMAGVSCARAMG
                     FQPYWLDLLGIPGITTDVINRCNTNYSYWSRISQRSKLFNKYVWEAVTTGEPTLPGLS
                     AFLNTWIPGWPWRFLKTNVKDYKMSELFKNLFQALPAEMPILTSHMWICMGAVAGGMT
                     NVVDMMFDNWPMAFQMTEGAKHGVQSPSGYYGFRVMRGFDENNAILKPMPADALFFTG
                     HHVDHELVENIETDCNARISRMKNSEPRRFLLTMGGAGAQRELFKAIIQHCIPLIQKD
                     KLALFVNLGDHKGNWDWLKSELAEYQEMINTHFSWQDTKSFTDSIRSEPARGLHVFLH
                     DNTFHAVYATNYLMRVVDVMITKPSELAFYPVPKIFNERVGGHEMWGAIRGAELGDGT
                     VETRSIPQTIQAIDILTEENDLLEIYCDCIVKNKHIGIYDGAYKCVELATGRKFERLT
                     NGSSVRIG"
     gene            5695..>5868
                     /locus_tag="AC16CB_12325"
     CDS             5695..>5868
                     /locus_tag="AC16CB_12325"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195126.1"
                     /note="catalyzes the interconversion of UDP-galactose and
                     galactose-1-P with UDP-galactose and glucose-1-P; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HIR3343302.1"
                     /translation="MTYQSYREIPHRRYNPLTGEWVQVSPQRTQRPWLGQVERGPDES
                     RPAYDPDCYLCPGN"
BASE COUNT         1405 a         1541 c         1469 g         1453 t
ORIGIN      
        1 ctgtgggccg gctcctggcc tgagtacgac gctgtcatgg gccccgccgt cgctgctgta
       61 ctcaccggcg aggagaccgt cgatgacttc gccgccagca tctgcgacga agccaataaa
      121 gccttcaacc agtaattcat ggctttgaag gtgcattgag ttttaatgcc gggcgggtga
      181 gcttgcacag aatatcgccc gcccggtgct ctctaccaga agttatcacc caaccgatag
      241 cagcgggcag caatcctctc taccggatct cacattgcat ccacacccgc ggtagctcca
      301 cagtaaaccg tggtcataaa caccaccatg caatcaaggc taactcagcc tgcgaaagcc
      361 ttcccggatg ttttgagggt cgctactcat gcggtggtat gagaaataga agtagctcct
      421 taacagtcca aatatgatcg gtaagacccg cagcgatggc tggggatcgt tgtacccagc
      481 ggaagccatg gctgtccacc caccgcttca agcgcaggct cttgtgctcg gggcagaaga
      541 tgtagacaca gcccatcaga tacgtgagtg gctccaagat tgcgggttgc ggatcagcgc
      601 tcgactgcgc cgtaccagga ctgccaggca agagcggaag gtggcgttca gccgctcgat
      661 gaaagcggtg ttgagcttgg ttctgccttg gctttttttc aacagcccct gagcagcttt
      721 cggacagcca tgcgctatca gggtcactac gatgaccatc caacgaatga tgcattcggt
      781 agaatggcga cccggtcgtt gcagcaaagg ccttaccata tatttgtatt ggtaacttgc
      841 ttcttttcgg ctatgaacaa cgataatgtt ctcacctact ttgccccttg cagggtaggt
      901 catattgggg cagaattgtt gtcgagggcc catgtggctt acgcttttca ggtggtagaa
      961 cctttagagt gccacaggac cttcattttt tcaacctcca cgttttattg gagtgctacc
     1021 acacgcacag gaggatgtta tgaaaggaaa aacccaaaca tcatccatcc ccaatcgaaa
     1081 acaaagctcg ggaatcttgc taactgtgct cgccatctgg catgtactcc tggcattagg
     1141 ctgcctagca ggtgcggttg tgttgtggca gcgctacaca cccgaagcaa cctggctaca
     1201 atggctgtat attattgtca tagttctcat cgcagcctca agcgccatca cggtgccgct
     1261 gatcttacgc cgcgagcacc gagggcgcat cctatcgctc gccattaact atctgggttt
     1321 ccttatctgc ctgtttgggt ccatgcatgc gcttggcata ttcgtgggca tcgactcgct
     1381 ggcaagcagc ttcgggcgtg gaattctatt ccttgctggc gtattatccg gttatctgat
     1441 cacgactatc ggcgatcgct acgaaaacca tcccggacgg gagcgattct tccgccgcct
     1501 tgggaagatc atcatggcga tcttcggcat actcttcttg tttgctgtag ggattttgcc
     1561 tgggattatc tcgctgttat cgcgctttaa ctccccggta acattagctc tgggtgttgg
     1621 cgtgctgcta tttgggctga tgatctgggc aatgtggcaa cagccaagcg cgcaggcaat
     1681 gggcgccaag aacatccatg aagaaatcct caacggttat ctatttttat cccctaactt
     1741 gttcggcttc ctatttttct ttgccggacc tttgctttta tcgttttatg taagcttcac
     1801 cgactgggat gctttcggct caaaaaattg gattgggctg acgaactatg ccaagattgt
     1861 taatcttgat attgccaccc tggaagatcc taaacaacgc gcttcagaga ttctggatgt
     1921 aaaggtctac gacgaactaa cccgtttcag cttgtttgga aaacatatca ttatcggggc
     1981 agaggataaa ctcttctggc tggcgctgcg aaacacattc atattcagcg tgctggctgt
     2041 cccgctgagt attattcccg cactattcct ggcgaattta ctgaacagca agctgccagg
     2101 catgaaattt tttcgagcca tctatttcct tcccagcatc gcagctgtag ttggtgtggc
     2161 tttgatctgg cagtggctgt acaacgcggc cgtaggttat attaactacg gtatcaccag
     2221 catggtgggc ttaataaatt cagtgacgga ttctgcgctc atcgaccccc aaatccgctg
     2281 gttatcggac acacgcaccg ctctacttgc tgttgtaatc atgtcagcct ggcaatggat
     2341 cggctttaac acggttttat tcctagccgg gttacaaaat atccccggcg aactctacga
     2401 agctgctacc gtagatggag ccgggcaatg ggcgaaattc tggaacataa ccatcccttt
     2461 gctggcacca accaccttct ttgtagttac taccgcaatc atccaggctc tgcagctttt
     2521 cgagcaagtc tatgtgctga tccccaccga accacccggg ggtcccaaca atgccacact
     2581 gtctttggta ctttacctct atcaaaaagg cttccagcgt tttgatcagg gctatgcctc
     2641 cgcggttgcc tgggtattgt ttgtggtcat tttcagtgtg acccttttcc aattcctgcg
     2701 ccagcgctcc ggaaggggcg cgtatagtgt ttgagttccg gaggccagtc atgcgctctt
     2761 atcatctggg cagattgata acaaacacac tggtgtatat catccttatc ctgtttgcat
     2821 ttttcatgct cttcccattt atttacatgt tctcaacctc cttgaaatac cagaaggaca
     2881 ctttccgcta tccgccgcgc ttgatgccta tgcaaggcat aaccacccag gtggaaggtt
     2941 ttgatgagcc gctgccgctt ttctatgtcg attacaatgg cgaagaacgc aaattcgctt
     3001 tgacagagag cgggatcaag atcggtattt atgctgagcc aaacaacctg gaggtgacct
     3061 acgagcgccc attgacccag gtcaagccaa ccggcggtgt agttaaccag gccattgtga
     3121 tggttaacgg aaaagaagag aaattattcg atgtagacgt cgaagggcag atgataccca
     3181 tgatcaaagt aggtcagaca gctcttggga attttataga tccgagcgac ccgtctatat
     3241 ttgtgcttca gaacgtccgc ctgagccaac ccgtcgaaga gccggctggc agaccggaaa
     3301 attacaaaga ggtgaccgag cttcagggac tggaccgcag cctgaccaac acagccttgg
     3361 tcaccatcct ggtagtaatc ggccagctag tgacatctgt gctcggcggt tacgcgctgg
     3421 cgcgccttcg cttcccgggc cgcgaccaga tatttgtcat ctatctgggc acgattatga
     3481 ttccattcgt tgtgctgatc accccccttt accagctaat ggtaatgatc ggctgggtgg
     3541 ataagctggc tgctctgatc atcccttgga tttttaccgc atacgggact tttctgatgc
     3601 gccagttctt catatcatat ccaaaagaga ttgaagaatc agcactgatc gatggcgctt
     3661 cgcggctgca aatcctggtg agcatcctac tgccggcaag tgtccccgcg ctggctgctc
     3721 tcgccacctt tacctttcta tacgcctgga atagcttctt ctggcctctg gttatcatca
     3781 acacaggcaa ccgcgccaac catgtgctga ctctttcact gagcatcctt cgtgggcgcg
     3841 ccagcgataa tgccaatctg gttatggcgg gcgcagcaat tgccatcctg ccgccgatga
     3901 tcatttatat cctggctcaa cgcttctttg tggaaagtgc taccagcagc ggggtaaaag
     3961 ggtaacaata accgcccatt accgttccgg ggtcagagag aggacgaagt atactctccc
     4021 cgtctcttta ttctgtgcgc ctttaatgta aaattctgca tcgggagcac tcaacattat
     4081 ctggcatcgc acaacggaga agtcttatga agataaaaga tggtcgtttt gtcgtctacg
     4141 ggaatcagat cagcagctca caatctaaaa aggcaataaa atggcagaac atgtttgcca
     4201 agaagttcaa gtatgacccc gatgagacat acaccttaag cctggtagac aatgaatatt
     4261 taggtccaat cttcggccta agagatatcg tacgcagtga ccagggggaa aaaattgagc
     4321 cggaaaatgc ggtcatctgc agcacgatcc gcatgggctt tggacactac cgaattgcaa
     4381 tggcaggtgt atcctgcgcc cgggccatgg gatttcaacc ctattggctg gacttgctcg
     4441 gaattcccgg catcaccaca gatgtgatca accgctgcaa cacaaactac agctactggt
     4501 cgcgtatatc acagcgcagc aagctgttca acaaatacgt ttgggaagca gtcaccacag
     4561 gcgagcccac tctgccgggc ctatccgctt tcttaaatac ctggatcccc ggctggccgt
     4621 ggcggttctt gaaaacaaac gtcaaagact ataaaatgag cgaacttttc aagaatctgt
     4681 tccaggccct gccggcggag atgccgatcc tgacttccca catgtggatt tgcatgggtg
     4741 cagtggcagg cgggatgaca aatgtggtgg acatgatgtt cgacaactgg ccaatggcat
     4801 ttcagatgac agaaggcgca aaacacggcg tccagtcacc ctccggctat tatggttttc
     4861 gagttatgag aggcttcgac gaaaacaatg ccatcctgaa acccatgcct gcggacgcct
     4921 tgtttttcac aggtcaccat gttgaccacg aattggtgga gaacattgag acagactgca
     4981 acgcacgcat ctcccgcatg aaaaacagcg aacctcgtag attcctgctc accatgggcg
     5041 gcgccggggc gcaaagagaa ctgttcaaag ccattatcca gcactgcata ccattaattc
     5101 aaaaagataa actggcatta tttgtcaatc ttggagacca taaaggtaac tgggactggc
     5161 tgaaatccga gttggcagag tatcaggaga tgatcaacac ccatttcagc tggcaggata
     5221 cgaaatcatt tactgacagc atccgatccg aaccagccag aggtctgcat gttttcctgc
     5281 acgataacac attccacgcg gtgtatgcta ccaactactt gatgcgcgtc gtggatgtga
     5341 tgatcacaaa acccagtgag ctggcgtttt acccggtgcc gaagatattc aacgaacggg
     5401 ttggtgggca cgagatgtgg ggtgccatcc ggggcgcaga actgggcgac ggcactgttg
     5461 agacacgatc catcccccag acgatacagg ctattgatat cctgactgag gaaaacgatc
     5521 tcctggagat atactgcgac tgcattgtga agaataagca tatcgggatt tacgatggcg
     5581 cgtataaatg tgtcgagctg gcaaccggta ggaaatttga gagattaacg aacggctcca
     5641 gcgtccgcat tggatgaaat tttatccatt ccaatatttc gtacgaggcc aagcatgact
     5701 taccaatcct atcgcgagat cccccaccgg cgctataacc cgttaaccgg cgagtgggtg
     5761 caagtctcgc cacagcgcac gcagcgaccc tggttggggc aggtagagag agggcccgac
     5821 gaaagccgtc cggcatacga tcctgactgc tacttgtgcc caggcaat
//
LOCUS       DBNGSX010000420         8777 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_108156, whole genome shotgun sequence.
ACCESSION   DBNGSX010000420 DBNGSX010000000
VERSION     DBNGSX010000420.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8777)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8777)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8777
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_108156"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3277)
                     /locus_tag="AC16CB_12330"
     CDS             complement(<1..3277)
                     /locus_tag="AC16CB_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3343303.1"
                     /translation="MLSSDNGNHNTKKPVGAALVVGAGIAGMQAALDLAESGIKVYLA
                     ERGPAIGGTMAQLDKTFPTNDCAMCIMSPKLVEVGRHLNIDLITCAEVDGISGEPGDF
                     VVSMRQRPRYVDSLKCTGCGDCATVCPISLPDAFNGGLSTRKAIFKLYPQAIPNAFSI
                     DKRGTAPCRDACPIHQRAQGYVALVSEGRYADAYRTIREDNPFPSICGRVCNHRCEDA
                     CNRAQADAPVNIMALKRFVADWAWENILDTPITGSPSVEGDGETGVSFPEARKTRGEG
                     ERPLSGRRVAVVGSGPAGLTCAQDLSGDGCEVTIFEALPVPGGMLRAGVPVYRLPPEV
                     IRREIDQIINNGIDLQLNHRIEDVESLLGEFEAVFVAIGAHAGVKLPIPGNDLPAVWM
                     ATDFLRQANLAVSGDLQPGESAVLHTQVQDQRVLVLGGGNVAIDAAMTAVRLGAKWVG
                     MTCLEGREQMPAHAWEVRDAEEEGIAVYPSRTFKEVTSSEGRCTGVRTVEVNFRGFID
                     GRPDFDEIPGTEQVIPADIVIFAIGQRPESDCLKRVKLLRGGRVSVDAETLATDVPGI
                     FAGGDAVTGTAFVVDAIAAGHRAARSIGAFLLGCEDLKLDRNQANVVKLDSHEVSQRI
                     AIGDASSAIRAETKKRPAAERKLDFNEIYAGLSEDEAKAEAMRCLRCGVCSECNQCVY
                     ACRAEAILHEDAERLTELNVGAVILAPGVEAMPGDIRPEYGYGRYPNVVTSVQFERML
                     SASGPFGGVVQRPSDGKHPLKVAWIQCVGSRDYTSLPDGTPRAPYCSSVCCMYATKEA
                     IIAKEHDPRIQPTIFYMDVRSFGKGFEGYIERARGEHGIRYIRCMVSSVSEAPGTKNL
                     RLGYVTYEGSNGRKPNLHEEEFDLVVLCVGLRPSPAARATAERLGVELNEYGFVAGQA
                     FEPSLTSCPGIYAAGAFAEPKDIPESVIEASCAAAQASALLGEARASQTRLPSFPPER
                     DISDEPEKVGVFICHCGINIGSVVDVPAVVEHAMTLPGVAYAEHNLYTCSQDTQERIR
                     QRIQENGLNRVVVASCTPRTHEPLFQDTLRAAGLNPHLFEMANIREQDSWVHRSKPQA
                     ATEK"
     gene            complement(3294..4190)
                     /locus_tag="AC16CB_12335"
     CDS             complement(3294..4190)
                     /locus_tag="AC16CB_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HIR3343304.1"
                     /translation="MESAFSPKQVQKEKRPVYSYYPGCSLHSTGLEYGQSTREVFRAL
                     EFELAELDGWNCCGASSAHALNHTLAYALPARTLALAQAMGRELVTPCAACFNRLKQA
                     DHALKSEPELRAEIESVVGFQYSGEVAIRPVLAVLFEDVPASRLVELVKFPLNGLKVV
                     PYYGCLLVRPPQVTRFDDPDHPQVMTKLLCSIGAEVMDWSQATDCCGAGLSLGRPDVV
                     QGLVGRLVERAREAGAQALVTACPLCQMNLEMRQTGPDKMPAIYFTELLGLAFGLPEA
                     KKWWPKHLIDPRPVLRMAGVSV"
     gene            complement(4191..4715)
                     /locus_tag="AC16CB_12340"
     CDS             complement(4191..4715)
                     /locus_tag="AC16CB_12340"
                     /inference="COORDINATES: protein motif:HMM:NF024581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3343305.1"
                     /translation="MITHLVSDVMRLSNQQVELCYHCHKCTAGCPVVGAMAYGPDRIL
                     RMAALDQFESIFQSKDIWLCAGCYTCTTRCPNSIDIAAVMDSLRQLALAEGYQAGEPD
                     IFVFHRLFLGVLQRLGRSHEAILLGLFKLRSHQPLMNDLGAGFGLVLRGKIPVLPQNV
                     KAAPPVRKIFQGTK"
     gene            complement(4840..6714)
                     /locus_tag="AC16CB_12345"
     CDS             complement(4840..6714)
                     /locus_tag="AC16CB_12345"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3343306.1"
                     /translation="MKIIDVSKPGFRTLVEAELVDSVAWLVRLRWFVGAGVLLGTWSM
                     EALLGLRAPVGPLYAIGAGILGYNLAFYLVERKFTQTSAATIAFRRLSVWQAVLDWLA
                     MIMLIHFSGGIESPMIFFFIFHIIIASIFFTPRTAFGFAILAAALLSGTAILEYYAIL
                     AHKPVVGFLDMPLYQNSLYIAAVLFFFTSTGLFSAYLATSIQKRLRERQEEIIRLSEG
                     LQRATMRLQTLNDGARIINSTLDLPEVLQRLVKNTVDAMGVRACSIRLLNKERQILEP
                     VAVNGLSQAYLNKGIIDAATNPVARRVLGGEVINIPDAPNSTLLQYPEEARQEGIRSI
                     ISAPLLGKSGPLGILRAYAIEPGRFSREDEAFLIAIAAQGSIAIENAMAYKAVEELDL
                     AKSKFIRTVTHELRSPVSVVHSLLRTITTGYAGQVSDQQMDILSRASRRVAYLQKLID
                     DLLDLAVGKADFWKSEDYEPIDIKAVVNKVVKRFEVLSQEKRLSLEFLDETNAGRARV
                     LATQDGMDRIINNLVSNAVKYTPSGGCVTTKLSIAADKVRLIVEDTGIGIPEDAVDHL
                     FEEFYRAPNARNVEREGTGLGLTIARDLVIRFGGSIQVERLQGAGSRFTVTLPVAAAS
                     "
     gene            complement(6744..7154)
                     /locus_tag="AC16CB_12350"
     CDS             complement(6744..7154)
                     /locus_tag="AC16CB_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018194949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3343307.1"
                     /translation="MSQKRILLIDDDPDLRLAIRLPLEASGYAVFEASSVVEARTAVK
                     EIKPDLIVLDVMMDTTTAGFQFALELHSTDPQSEFSEFGQVPIIMLTAIHSTTPLRFA
                     PDEDYLPVQTFIEKPIEPDVLLSKVREMLGESVN"
     gene            complement(7151..7624)
                     /locus_tag="AC16CB_12355"
     CDS             complement(7151..7624)
                     /locus_tag="AC16CB_12355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HIR3343308.1"
                     /translation="MNPESGTLVLGLGNPLRRDDGLGGRVIEMLAESKLPAQVVLIDG
                     GTPGWGVTTFLQGWSRVIVIDAADMGSMPGKWDRCQLDAVSLDVGSGHFSLHEPGLSE
                     GLALGQALGTLPQDIVLYCVQPERTEEGIGLTPVVEAVLPELVENILIDLWNKRV"
     gene            complement(7621..>8777)
                     /locus_tag="AC16CB_12360"
     CDS             complement(7621..>8777)
                     /locus_tag="AC16CB_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HIR3343309.1"
                     /translation="QITSRICGICSTGHTTTSLRATEKALGVVPSEQTRLLRKLAFYG
                     EMLDSHVLHVYMLVAPDFLGVGSVIPLASTHPEVVLRALRMKKMAGDLCAAINGRHTH
                     PIAMTVGGFTHFPNPDELEDLRYRLEEARRDVDATVDLFASLAFPNFERETEYISLSH
                     PDEYAFIDGEIVSSDGVTLPVEAYRDITNEYLVEHSSAKLARHQRDSYMVGALARFNN
                     NYAQLHSKAKKAASALGLRPKVINPFLNTAAQVVEIVHCVEGAIAIIDQLLERGIQPE
                     EPAPPQSLEGEGISGCEVPRGVLIHHYWIGADGFLSGADCVIPTNQNIASIESDMRAL
                     VPSIINNSVNDIQLELEMLVRAYDPCISCSVHVVKIDPLLKVGDAIKNRL"
BASE COUNT         2095 a         2610 c         2227 g         1845 t
ORIGIN      
        1 ctttttccgt ggcagcttgc ggcttggagc ggtgcaccca cgaatcttgc tcgcggatat
       61 tggccatctc gaacaaatgc gggttgagtc cagccgcccg tagcgtatct tggaagagcg
      121 gctcatgggt gcgcggcgtg caagaagcca caaccacacg attcaagcca ttttcttgga
      181 tgcgctggcg aatgcgctcc tgggtatctt gcgagcaagt gtataggttg tgctcggcat
      241 aggccacccc aggcaaggtc atggcatgct caaccacggc gggcacatct accacagagc
      301 caatattgat gccgcaatgg cagatgaaca ctcccacctt ctcgggctcg tcagaaatat
      361 ctcgctcggg ggggaaagaa ggcaagcggg tctgtgaggc gcgcgcctca cccaacagtg
      421 ccgaggcctg ggcagccgca caggaggctt ctatcaccga ttcagggata tcctttggtt
      481 cggcaaacgc acccgcggca tagattcccg ggcaagaggt gagactcggc tcaaaagctt
      541 gaccagcgac aaagccgtac tcatttagct caacccccag gcgttctgcg gttgcacgcg
      601 ccgcaggtga aggtctcaaa cccacacaca gaacgaccag gtcaaattct tcctcatgca
      661 ggttaggctt gcgcccattg cttccctcgt aagttacata gccaaggcgc aaatttttcg
      721 tcccaggcgc ttcgcttacc gacgagacca tacagcgtat gtagcggatc ccgtgttcac
      781 cacgggcgcg ctcgatatag ccctcgaacc ctttgccaaa ggagcgcaca tccatgtaga
      841 aaatggttgg ctgaatacgg ggatcatgct ccttggcgat gatggcttct ttcgtcgcgt
      901 acatacagca caccgatgag caatatggcg cgcgaggtgt gccatccggc aggctggtgt
      961 aatcgcgcga gcctacacat tgtatccagg ctacttttag gggatgtttg ccatccgaag
     1021 ggcgctgcac cacaccaccg aagggtccag aggcggagag catgcgctcg aactgcacac
     1081 tggtgacgac gttgggatag cgcccataac catactccgg gcgaatgtcg cccggcatag
     1141 cctccacacc tggagccaag atgaccgcac cgacattcaa ctcagtcagc cgctcggcat
     1201 cttcatgcag aattgcctct gcgcggcagg cataaacaca ctggttgcac tctgagcaaa
     1261 caccacagcg caggcagcgc atcgcctccg ccttggcctc gtcctcgctt aagccagcgt
     1321 atatctcgtt aaaatccagc ttgcgctcgg ccgcggggcg cttcttagtt tcagcgcgga
     1381 ttgcgctaga tgcatctcca atggcaatgc gctggctgac ttcatgcgag tcgagcttaa
     1441 caacattggc ttgattccga tcaagtttca gatcctcaca gccgagtaga aaggcaccaa
     1501 tggagcgggc tgcgcggtgc ccggccgcga tggcatccac cacgaaagcg gtgccggtga
     1561 ccgcatcccc accagcgaag atgcccggca catcggttgc cagcgtctcc gcgtccacag
     1621 acacccgccc gccacgaagg agcttgactc gcttcaggca atccgattcg ggtcgctgtc
     1681 caatggcaaa aatcacaata tctgccggta tgacctgctc ggtgccggga atctcatcaa
     1741 aatcaggccg cccatcgata aacccgcgga agttgacttc aaccgtgcgc acacctgtac
     1801 atcgaccctc tgaactggtc acttctttga acgtgcggga tgggtagact gcgatcccct
     1861 cttcctctgc gtcccgcacc tcccaggcgt gagcaggcat ctgctcgcgc ccttccaggc
     1921 aagtcatccc aacccatttg gctcccaggc gtactgccgt catggctgca tcgatggcca
     1981 cattaccgcc tcccaggacg agcacccgct ggtcttgtac ctgggtgtgc agcactgcgc
     2041 tttcgcccgg ctgcagatcg ccgctgacag ccaggttggc ctggcgcaga aaatcggtcg
     2101 ccatccatac tgcaggcaga tcgttgcccg gtatgggcaa cttcacgccg gcgtgcgcac
     2161 cgatggcgac aaagaccgcc tcgaactcgc ctaaaagcga ttcgacatct tcaatccgat
     2221 ggttgagttg aagatcaatc ccgttattga tgatctgatc gatctcccgg cggattacct
     2281 cagggggcaa gcggtagaca ggcaccccag cgcgcagcat accgcccggc acaggaagcg
     2341 cctcaaagat ggttacttcg caaccatctc ccgacaaatc ctgagcacag gtcaaccctg
     2401 ccgggcctga tcccaccacc gccaccctgc ggcctgacaa ggggcgctcg ccctcgcccc
     2461 ttgtctttct ggcctcagga aaggacacgc ccgtttctcc atctccttcg acggaggggg
     2521 agcctgtgat gggagtgtcc aatatatttt cccaggccca atcggccaca aagcgcttaa
     2581 gcgccataat atttaccggg gcatccgcct gggcgcggtt gcaggcatcc tcacagcgat
     2641 gattgcacac tcgcccgcaa atcgagggga aggggttatc ctcacggatg gttcggtagg
     2701 cgtcagcata gcgtccttcc gagaccaaag caacatagcc ttgcgctcgc tggtgaatcg
     2761 ggcaggcatc ccggcagggg gcagttccac gcttgtcaat ggaaaatgcg ttagggatag
     2821 cttgcggata aagcttaaaa atggcttttc gagtggagag cccaccgttg aacgcatcag
     2881 gcaagctgat agggcaaacc gtagcgcaat ctccacagcc agtgcatttc aaactgtcga
     2941 catagcgcgg cctctggcgc atgctgacga cgaagtcacc cggctcaccg ctgataccat
     3001 caacctcagc acaggtgatt aaatcgatgt tgagatgccg tccaacttca accagcttgg
     3061 gagacatgat gcacatggca caatcgttgg ttgggaaagt cttatcgagc tgcgccatgg
     3121 taccgccaat tgctggcccg cgttcagcca ggtaaacctt aatacctgac tcggccaaat
     3181 caagggcagc ctgcattcca gcgatgcccg cccccaccac cagggcagcc ccaacgggtt
     3241 ttttcgtatt gtgatttcca ttatccgaag agagcataat cccaatctcc accttagaca
     3301 gacacgcccg ccatgcgcaa aactggccgg ggatcaatca ggtgcttagg ccaccatttt
     3361 ttggcttcag gcaacccaaa cgccagccca agcaactccg taaaataaat cgcaggcatc
     3421 ttatccgggc cagtttgccg catctccagg ttcatctggc ataatggaca ggcagtcacc
     3481 agcgcctgag ctccagcttc acgagcgcgc tcaaccagac gccccaccaa cccctgaaca
     3541 acatccgggc gacctaatga aagcccagca ccgcagcaat ctgtggcttg agaccagtcc
     3601 atcacctccg ccccgattga gcagagcaac tttgtcatga cctggggatg atcggggtcg
     3661 tcgaaccggg tcacttgagg ggggcgcacg agcaagcagc catagtaggg aaccactttc
     3721 aatccattca ggggaaattt caccaactca acaaggcgac tggcgggtac atcttcaaaa
     3781 agcaccgcca gcaccggtct gatagccacc tccccagaat attgaaaccc caccacagac
     3841 tcgatctcag cgcgcaattc gggctcgctc ttgagcgcat gatcggcttg tttgaggcga
     3901 ttgaaacaag ccgcacaagg tgtcacgagt tcgcgtccca tcgcctgcgc taaggccaat
     3961 gtgcgggcgg gcagggcata tgccagcgtg tggttcaggg cgtgtgccga cgaagcacca
     4021 caacagttcc agccgtccaa ttcagccagc tcaaattcca gcgcacggaa gacctcacgg
     4081 gtggattggc catactccag accagtcgaa tgcaaactac agccgggata gtaagaataa
     4141 accggacgct tttctttctg aacttgcttt ggagagaatg cgctttccac ttatttagtc
     4201 ccttggaaga ttttacgaac cggaggcgca gccttgacat tctgtggcag cacggggatt
     4261 ttccccctca gtacgagacc gaaccccgca cctaaatcgt tcatcaatgg ctggtgagag
     4321 cgcagcttga agagacccag caggatcgcc tcatgggagc gcccaagacg ctgaagcaca
     4381 cctaaaaaca ggcgatgaaa aacaaatata tccggctcac ctgcctgata accttcagcc
     4441 aaggcaagct ggcgtagcga gtccatcacc gcggcaatgt cgatactatt tggacagcgt
     4501 gtggtgcagg tatagcaacc cgcacacagc cagatatcct tgctctggaa gatcgactcg
     4561 aactgatcca aggctgccat acgcaggatg cggtccgggc cataagccat cgcgccgacc
     4621 accgggcagc cagccgtgca cttatgacag tgatagcaga gctcaacttg ctggttcgac
     4681 aaacgcataa catcagaaac caggtgagta atcacaggca cacctatcta ttaaccttat
     4741 tactaattga agcgggaaaa aatactcaat tctggagtta attacaccgg ccgatcttaa
     4801 tactcaaacg ccacttcgcc atgcccccat acagaggctc tatgatgcag cagccactgg
     4861 caaggtcacc gtgaagcggc tgcccgcccc ttgaagcctc tcaacctgaa tcgagccgcc
     4921 aaagcgtatc accaggtctc gcgcgatggt gagccctagc cctgtaccct cccgctcaac
     4981 attgcgcgcg ttgggagccc tgtaaaattc ctcaaacaga tgatccacgg catcttcagg
     5041 aattccgata cctgtatcct caactatcag ccgcacttta tcagctgcga tagaaagctt
     5101 cgtcgttaca cagccccctg aaggggtgta tttgacggca tttgacacca gattattaat
     5161 gatgcgatcc ataccatcct gcgtcgccaa cactctcgcc ctacctgcgt tggtttcgtc
     5221 caagaactca agggaaagcc ttttctcctg gcttaatacc tcaaaacgtt tgaccacctt
     5281 attcactaca gcctttatgt caataggttc ataatcctcg gacttccaga agtccgcttt
     5341 ccccaccgcc agatctagca aatcgtcgat caatttctgc aaataagcca cacgacggct
     5401 ggcgcggctg agaatatcca tttgctggtc agaaacctgc ccagcataac cagttgtaat
     5461 agtgcgcagc agactgtgga cgacgctcac cggcgaacgc agctcgtggg tgacggtgcg
     5521 aatgaacttg ctcttcgcca gatccaactc ctcaacggct ttataagcca tagcgttttc
     5581 gatagcgatg cttccctgag cagcgattgc tatcaagaag gcttcatcct cccgtgaaaa
     5641 gcgaccaggc tcgatcgcat atgccctcag gatacccaac gggccactct ttcccagcag
     5701 cggtgcggat ataatcgatc ggatgccctc ctgccgggct tcttccgggt attggagcag
     5761 cgtgctatta ggagcgtcgg gtatattaat cacctctccg cccaataccc ggcgggcaac
     5821 tggattggta gctgcatcga taataccttt attgagataa gcctggctta agccattcac
     5881 agccacaggc tccagtatct gtctctcttt attcaacaag cggatggagc aggcgcgcac
     5941 ccccattgca tccaccgtgt tcttcaccaa acgctgcaat acttcgggga gatccagcgt
     6001 tgagttgatg attctggctc catcattgag cgtttgcaag cgcatcgtag cccgctgaag
     6061 accctcgctc aggcggataa tctcttcctg tctttcgcgc aacctctttt ggatggaggt
     6121 ggccaggtaa gcggagaata gtcccgtact ggtgaagaag aacaagaccg cggcgatata
     6181 aaggctattc tgatataacg gcatatccag aaagcctaca accggcttgt gggcgaggat
     6241 ggcatagtat tctagaattg cggtccccga aagcagtgca gctgccagga tcgcaaatcc
     6301 gaaggctgta cgcggcgtaa agaagatcga ggcgatgatg atatggaaga taaagaagaa
     6361 gatcatcggg ctctcgatcc ctccggagaa gtggatcagc atgatcatcg ccaaccaatc
     6421 caagaccgcc tgccaaaccg ataggcgccg gaaggcaatc gttgccgccg aggtttgggt
     6481 aaattttcgc tccaccaggt aaaatgcgag attgtagcct aaaatgccgg ccccaatggc
     6541 atacaagggc ccaacaggag cacgcaaccc taaaagggct tccatgctcc aggttcccaa
     6601 aagcacgccc gcgccaacaa accagcgcag gcggacgagc caggcaacgc tatcaactag
     6661 ctcagcttcc accagtgtcc ggaaacctgg ttttgaaaca tctatgatct tcatatttga
     6721 cccaggggaa gtgaaacttt ccatcagttt actgattcgc caagcatctc gcgcactttc
     6781 gatagcaaca catccggctc gatgggcttc tcgatgaagg tttggacggg aaggtaatcc
     6841 tcatccggag caaagcgcag cggtgttgtg ctgtggatgg cagtcagcat gatgataggc
     6901 acttgaccga attcactgaa ttctgattgc gggtcggtgc tgtggagctc cagggcaaac
     6961 tgaaagccag ctgttgtcgt atccatcatc acgtccagca cgatcaggtc tggcttaatt
     7021 tccttaactg cggtcctggc ttcgaccaca ctgctggctt cgaatactgc atacccagag
     7081 gcttccaacg gcaggcggat tgccagccgc agatctggat catcatcgat cagtaggatt
     7141 ctcttttggc tcatactcgt ttattccata gatcaataag tatattttcg accagttcgg
     7201 gcaaaaccgc ctcaacgaca ggtgtgaggc cgatgccctc ttcggtgcgc tcaggctgca
     7261 cgcagtagag gacgatatcc tgcgggagcg tcccaagggc ttggcccaaa gccagaccct
     7321 ctgacaaccc tggttcgtgg agggagaaat gtcccgaacc cacatccaaa ctcacagcat
     7381 ccaattggca gcgatcccac ttcccgggca tagaccccat gtcagccgcg tcgatgacga
     7441 tcacccgcga ccaaccctgt agaaaggttg tcactcccca tccgggggtg ccaccatcga
     7501 ttagcacaac ctgggcaggc agtttactct cagctagcat ctctatcacg cgcccaccaa
     7561 ggccatcatc acgacgcagt gggtttccca gaccaagaac taaggtgccg ctttctggat
     7621 tcataggcga tttttaattg catctcccac cttcaagagg ggatcaatct taacaacatg
     7681 cacggaacac gagatacagg gatcataggc gcgcaccagc atctccagct ccaattgaat
     7741 atcgttcaca gaattattaa taatactagg gacaagggcg cgcatatcgc tttcaatgct
     7801 ggcgatattt tggttggtag ggatgacaca atcagccccg ctcaaaaaac catccgcccc
     7861 aatccagtaa tggtgaataa gcaccccgcg ggggacttca cagccgctaa taccttcgcc
     7921 ctcgagcgat tgtggcgggg caggctcctc tggctgtatg cctcgttcca acaactggtc
     7981 gataatggcg attgcacctt caacgcagtg tacgatctcc accacctgag ccgcggtatt
     8041 cagaaatgga ttaatcacct ttggtctcaa tcctaaggcg ctagcggctt ttttagcctt
     8101 cgaatgaagt tgtgcataat tgttgttgaa acgcgccagc gcacctacca tgtacgaatc
     8161 ccgttgatgg cgcgctagct tagcgctgga atgctccact aggtattcgt tggttatatc
     8221 tcgatacgcc tcaactggca gggttactcc atcgctgctg acaatctcgc catcgataaa
     8281 agcatattcg tccgggtgtg acagagagat gtattctgtc tcgcgctcaa aattcgggaa
     8341 cgccagtgat gcaaataaat ccactgtcgc atccacatcc cgcctagctt cctccagccg
     8401 ataacgcaaa tcttccaact catccggatt tggaaagtgt gtaaacccgc ctactgtcat
     8461 cgcaatcggg tgggtgtggc gcccgttaat ggcagcgcat agatctccgg ccatcttctt
     8521 catgcgcaat gccctcagca cgacctcggg gtgcgtgctc gccaaaggga tcacactacc
     8581 cacgcccaag aaatctgggg cgacaagcat atacacgtgc aatacgtggc tatccagcat
     8641 ctcgccatag aaagccaatt ttcgcagcaa tcgggtctgt tcgctcggca ccacacccag
     8701 agccttttct gtcgctcgga gtgaggtggt ggtgtgccct gttgaacata tcccgcagat
     8761 acggctggtg atctgag
//
LOCUS       DBNGSX010000421        17840 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_870663, whole genome shotgun sequence.
ACCESSION   DBNGSX010000421 DBNGSX010000000
VERSION     DBNGSX010000421.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 17840)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17840)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17840
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_870663"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..3076)
                     /locus_tag="AC16CB_12365"
     CDS             complement(215..3076)
                     /locus_tag="AC16CB_12365"
                     /inference="COORDINATES: protein motif:HMM:NF013106.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR3343310.1"
                     /translation="MPSPAQITSLRRHRHNRAGRYSKTWFGVGCSTFLSLFAVLVGMM
                     GVWGYITLFHNLPSLDTLPALLDPPDGLLLQPTRLYDRTGTHVLLELENPAAVDRQVL
                     TLQSNQPNYLPSSLISATLATADPQFWSHAGYSFDGLTSGTHPTLAQTLVSDLLLWSE
                     PASLRRAVRERLLAAQITARFGRQQVLIWYLNSANYGRLAFGADVAARVYFNKPAAEI
                     NLAEAAVLAAVMQSPALNPLDAPNVVRERAVDVIQTMLEGGWINAAQASQARKSNLVF
                     RTQTETLANPAPIFINLVLEQLGQELSLNRLERGGFQIVTTLDQDLQFQAACALQLYT
                     ARLQGMPDPPNTMNGDECRSALLLPTSFTGQSMTVKDIKGNLVILDHRTGEILAMIGE
                     ISPGLDPAHYPGHPAGSLLTPFIYLTGFTRGLNPASMLWDIPSGSNKSFNLDGSFHGP
                     LRLRTALANDDLVPTEQVSTQVGAENVWRLAKQFGLEAARLPDDASVTDLLQESEVTL
                     LDISRAYGVLANQGLLAGRENTLSTGNTPPFTAAATIIRLEDATGKVWINHANNASHP
                     VVSPQLAYLMTHILSDEPARWSMLGHPNPLEIGRPFAAKLGRTGMEQDVWAVGSTPYM
                     TIGIWLGQASQGKISVQEAAALLHAVAQYATRNHAPDGWSMPAGINSLAVCDPSGMLP
                     TPDCPTVVNEIFIAGTEPTSFDTLFRRLQINRESGMLATVFTPPELVEERVYMQVPPE
                     AHEWARQAGLPTPPDTYDVILADTSPSADVKISSPSMFAYVGGKVRIMGTARGSNFEL
                     YRLQVGRGLNPKNWLQVGQDSQLPVSDNLLGSWDTQGLSGLYSMQLLVIQQDQRVQSD
                     VIQVTVDNQSPELSILYPTDGQIVPLSNQTITLQAQVNDDLALKNVEFYIDDRLVSNL
                     TQPPYTITWQARLGAHSLRIKAVDQAGNTAEARIAFKVE"
     gene            complement(3083..4810)
                     /gene="recN"
                     /locus_tag="AC16CB_12370"
     CDS             complement(3083..4810)
                     /gene="recN"
                     /locus_tag="AC16CB_12370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR3343311.1"
                     /translation="MLKELHIENFAIIDKIDLQLGAGLITFTGETGAGKSILIDAVET
                     LLGGRADATMVRSGCERAYVEGRFYISPPVREPVHSILKREELLDDPEYVTLGREIRL
                     NERNIARLNGRSVNATLLREIGEYLVDVHGQSEHLSLLRVRQHLGLLDNYAGLEDLLG
                     GYQKTYHKLQAVRRELGELRQAEQEAARRIDTLNYQINEIEAAHFRPGEEETLRDERN
                     RLANAEGLASLANEALQALDEGTSETPAVTDLFGQAVHALSNLAKLDPSQDNLSNQGQ
                     LLFDSLSEMVREIRSYLEGIEFNPKRLDQVEERLGLLQNLKRKYGESIEAILQYAERA
                     RQQLDAITHASERIQELEFDEAKLLSQIGIQGSMLSEKRREQAEKLGRSLENELYDLQ
                     MSGTRFEVEFRSRPDPNGAPLRDGQRVAFDSSGLEQAEFLIAPNPGEGLKPLVKIASG
                     GETSRLMLALKHVLTSADQIPTLIFDEIDQGIGGRVGAVVGQKLWRLAQKHQVLCVTH
                     LPQLAAFGTQHFHVEKLVQEGRTMTRVSQLTGNLRVEELAQMLGGISEGTLQSAREIL
                     QAVDNQTTH"
     gene            complement(4829..5350)
                     /locus_tag="AC16CB_12375"
     CDS             complement(4829..5350)
                     /locus_tag="AC16CB_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B-box zinc finger protein"
                     /protein_id="HIR3343312.1"
                     /translation="MTDIPATLYCENHPQTETNLRCNRCSKPICPKCAISTPTGYRCA
                     ECVRNQQKLFETSRWYDYVSATILAGLLSLVGSRIITSFGFFTILLAPVAGVIIAEVV
                     RFAIQRRRSKLLFQIAAIATAVGSSPFLLIGLLILLQGNLGGLWSVLWQGVYTFIVTS
                     TVYYRLGGIQMRV"
     gene            complement(5347..6207)
                     /locus_tag="AC16CB_12380"
     CDS             complement(5347..6207)
                     /locus_tag="AC16CB_12380"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039482.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD+ metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR3343313.1"
                     /translation="MPQLRRIAVVAHPELDDAYLEAERIATFLKQKDVQVSYGLLYDD
                     ELRTQIKTGAFELLIALGGDGTMLRAGHLCGPLGLPILGINLGHFGFLMEVRQNQWMD
                     ILPRLLTGDYWLEQRMMLNAELYRSDEILGTWDVLNEVVVGRGQTVRPVQLVAEVDGR
                     YLTTYVADALIAATPTGSTAYALAAGGPILPPELRNILLVAVAPHLSIDRAIVLAEGS
                     SVGITIHSTHHAVLSVDGQTPINVQDGDKVIARASDYTLKFVRFQDPGYFYRNLTPHM
                     EQNPTTGNQR"
     gene            complement(6240..6752)
                     /gene="cas4"
                     /locus_tag="AC16CB_12385"
     CDS             complement(6240..6752)
                     /gene="cas4"
                     /locus_tag="AC16CB_12385"
                     /EC_number="3.1.12.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HIR3343314.1"
                     /translation="MLPIAALAALLALVLLWLVNRRQRAAGLPPGKIIYADTRSWSPT
                     PEPLYDPDLRLTGKPDYLIKHKKKIIPVEVKSTRAAQSPYDSHIFQLATYCLLIERTF
                     GVRPPYGILHYPDRTYQIEYTSKLESSTLALLEEMRDQEKLHELPRTHESPARCRSCG
                     FRSICDVRLS"
     gene            complement(6761..7033)
                     /locus_tag="AC16CB_12390"
     CDS             complement(6761..7033)
                     /locus_tag="AC16CB_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343315.1"
                     /translation="MQNQQPHIYKHMTIIRSSEIGTYLYCQRAWWYRKSGYEPQNQAE
                     LAAGIHAHDQHGSKVQKADTLRKLAYFLLLAAGILAIVSLILQFSS"
     gene            complement(7042..7674)
                     /locus_tag="AC16CB_12395"
     CDS             complement(7042..7674)
                     /locus_tag="AC16CB_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HIR3343316.1"
                     /translation="MQNLDDIAERIRQSFETRTLVRDQALTRARTLTRHCANAIRAIH
                     RDERANAQEHLGAARDLANSIHKDLADFPDLYYTGYTQDALKEFAEANSVFALVYDQP
                     LPSPDELRLEYATYLQGLAEAAGELRRRCLDILRHGHSEEAERLLSHMDDIYAVLVTM
                     DFPDAITGGLRRLTDIVRGVNERTRGDMTLSLRQEHLEQSLKRLEKRFES"
     gene            complement(7680..8021)
                     /locus_tag="AC16CB_12400"
     CDS             complement(7680..8021)
                     /locus_tag="AC16CB_12400"
                     /inference="COORDINATES: protein motif:HMM:NF023376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HIR3343317.1"
                     /translation="MPSSSPPRPAAPNLPPLEVPPDFPIEYANLVRIAHSPSEIVFDF
                     AQLLPGGTPVRLQSRIVMSALGAKLFFRALADNLGKYESAYGEIKIPSGSTLADQLFR
                     PPQPPENPPQR"
     gene            complement(8228..8644)
                     /locus_tag="AC16CB_12405"
     CDS             complement(8228..8644)
                     /locus_tag="AC16CB_12405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR3343318.1"
                     /translation="MYHTIIVVGNLGRDPEMRYTPGGSAVTNFNIATNRQYTASDGTP
                     VKETIWFRVSTWGKTAEVCNQYLRKGSKVLVEGRLIPDQASGGPRVWTRQDGTAAASF
                     EVSANTVRFLSTRGEAEGEIPFGEGEVSANDDDIPF"
     gene            complement(8807..9787)
                     /locus_tag="AC16CB_12410"
     CDS             complement(8807..9787)
                     /locus_tag="AC16CB_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343319.1"
                     /translation="MITAEDLIHLPYTPGLTSAGVLQARRSLALGLVQADNLHIAPLR
                     RLVTAVAGELALRRHLTEARIPFKTLQTTPFTQSDWGDVVLGGRRCHLVEILYSRRSI
                     LRHIKDQHSSILQTPACLTDVYLTSELLEHHDLLVFIIILAKPQPSWYEIRRYPEKIL
                     PCHFAYILPKAWKNPAPGRVYGQISLTNEGESEFVIELGGRGVKGQYQSEHLCLPAHR
                     SVNVAQNYTALAYLSSETIPAVQLSLSRAEPDRPLRIPAHRWVNLWFNGVEIILAGYM
                     TVGDFHSKARRLSPTDRTWDDLPVQTPAKFLHISELHSLPQFFAWINSNA"
     gene            complement(9829..10683)
                     /locus_tag="AC16CB_12415"
     CDS             complement(9829..10683)
                     /locus_tag="AC16CB_12415"
                     /inference="COORDINATES: protein motif:HMM:NF013704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HIR3343320.1"
                     /translation="MAKRRLVNRLNDLSPKEWLKFQKSWFVHNPPPRRKDVLRHPAKF
                     PETLAQEFIEFFTRRGEVVLDPMVGTGSAMVAALRSGRHSYGVELNPAYAAIAQQVVS
                     EEQALLGEQARDLTAEVIVGNAADIAHYADTLQIPPADYVLTSPPYWDMLRARGAQTQ
                     KNRRASPELDVFYSDDPADLGNLSDYDEFLRRLVDIYAALKPFVKQGAYLTIIVKNVK
                     KGGVIYPLAWDLGRLLGQFYTLKDERIWCQDNQRLAPYGLGSAWVSNTFHHYCLQFRN
                     DLEISDKG"
     gene            complement(10693..11454)
                     /locus_tag="AC16CB_12420"
     CDS             complement(10693..11454)
                     /locus_tag="AC16CB_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343321.1"
                     /translation="MLKYKLWLVLSMILAGCLSRPTALPPEPTSTATPTGTPTATVIW
                     FPATATQTPLPTLDVSPTVDQLPPTGDLIFSDDFNDPDEWLLGQTSTTSIALGNQALT
                     LALNQPSGYLYTLRKSPILSDFYLEVTASPSLCRGDDEYGLLLRVSPSAEFYRFSLSC
                     EGSIRLDKYYNGKASSPQAKTPSGSVPRGAPSSSLISAWVKGKEMQFYVNNEYQFTVR
                     DPSLQEGSLGFFVRSAGDTALTVSFTNLSVRTIPN"
     gene            complement(11462..12754)
                     /locus_tag="AC16CB_12425"
     CDS             complement(11462..12754)
                     /locus_tag="AC16CB_12425"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIR3343322.1"
                     /translation="MPLDIIVGTQWGDEGKGRIVDLLSAHADYVARYNGGDNAGHTVT
                     VADQTFKLHLIPSGVIHPHTVGVIGNGVAINPATLLDEMNMLREAGVRIDPERLRLSY
                     AAHLITPAHRAIDAAQEAARGKGAIGTTLRGIGPAYTDKVARSGLRLGDVLDSRSFVP
                     ALQAHIEAANQRLTQLYHAEPLDVAATTHEYIQFTQQLQPYIQDVSALLSKALKDGQR
                     VLAEGAQGTLLDLDHGSYPFVTSSYPSAAGALLGLGLGVGFVERVIGVTKAFQTRVGA
                     GPFPTELSGDLALHLRGTGEQPWDEYGTTTGRPRRVGWLDMVLLRYALRINGLTELAL
                     TKLDILTGLEPLRICTAYRKGGQVYKDLPLGPGELAGFEPVYEELPGWQNDLRSTRTW
                     EELPPQSRAYILRIEELSSVPVRTVSVGPERQQVVEIS"
     gene            12903..13916
                     /locus_tag="AC16CB_12430"
     CDS             12903..13916
                     /locus_tag="AC16CB_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3343323.1"
                     /translation="MRKKVIMITGAAGEIGNALITSLAEQSNVPILSLDLQPLPEKQA
                     RMTTHLQGDVLDQALLSRLISEYEIDTIYHLAALLSTRSEFTPEAAHRVNVEGTLVLL
                     RLATEQSAWRGTPVKFIFPSSIAAYGMPDLATKKAYARVREWEWNYPRTMYGCNKLYC
                     EQLGIYFSRHYRQLAADRSVMLDFRSLRFPGLISAFTAPSGGTSDYGPEMLHAAAKKV
                     PYACFVREDVQIPFMAMPDAVTALLRLTESPHEALSRLVFNVTSFSLTAAEFREWVLK
                     YFPDAQISFDPDVKRQGIVDSWPADLDDSAARRDWGWQPDYDLERSFSEYLVPNIMKR
                     YSA"
     gene            13988..15154
                     /locus_tag="AC16CB_12435"
     CDS             13988..15154
                     /locus_tag="AC16CB_12435"
                     /inference="COORDINATES: protein motif:HMM:NF049034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysS/YqeB C-terminal domain-containing protein"
                     /protein_id="HIR3343324.1"
                     /translation="MDITDYPEVKPGPIVLFGSGETAPSGRKIFDHVMRQLPPKPKLA
                     LLETPAGFELNSSQVIGRVGEFISHRLQNYDPQITIVPARKRGTAFSPDDPDLIEPLY
                     EADMIFMGPGSPTYAVRQLRGSLAWHALLARHRLGATIVFASAATVAASLFSIPVYEI
                     YKVGEELHWKDGLDFFGLYGLPLVFVPHWNNNDGGEELDTSRCFMGKPRFAELMEMIP
                     SELSILGIDEKTALVMDPQSCACRVIGLGGVTIIHAGPAHRSATQPSQLDGTGLNEVA
                     ENRKAHVHHYQHGQAFSLKRIGPFCDPEAGAGLPGEVWQRALEVHHARQSQEMASFED
                     ESPPPEVLVLVEERQAARARKDWAASDSLREQVADLGWLIQDTPEGPKVVKSDD"
     gene            15188..16087
                     /locus_tag="AC16CB_12440"
     CDS             15188..16087
                     /locus_tag="AC16CB_12440"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009769276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3343325.1"
                     /translation="MTQTLSVGYIGLGLMGKSIARNILKAGFPLVVHNRSQAAVEELV
                     AEGAVRAGSAAEVASSVDVVFTNLPDTPDVEKVALGSNGVIEGAHPGLVFVDNSTIQP
                     AAARRIAEVLGEKGVQCLDAPVSGGDIGARQGTLAIMVGGPAEALDRVMPIFEAMGKT
                     ITHVGGAGTGQIAKAANQIMVAAQMVAMGELLVFAQKAGADPRKVVEAIQGGAAQCWT
                     LDVKPQRLFAGNRQPGFKAYMQAKDLGIVMESARQYGIPLPAAAVHTQLFNAMLQMGM
                     SELDNSAVLAVIEQLAGIRLSEG"
     gene            16171..16773
                     /locus_tag="AC16CB_12445"
     CDS             16171..16773
                     /locus_tag="AC16CB_12445"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3343326.1"
                     /translation="MNDTESSWLAQAQKGDAEAFSRLIETYQRPVYNLCYRMLNDAGD
                     AEDAAQETFLRAYKSMKRYDQSRPFPTWLLSIAAHYCIDQIRKRRMAIVSTEDLPYPD
                     LSDDAPGPESVVSRKEEQQRVRVLLDTLSPTDRAAVVMYYWYDFSYEEIGEALSLTLS
                     AVKSRLHRARLSMAQEWIDQQPQAGLAERKQHGKAQSPAF"
     gene            16745..17293
                     /locus_tag="AC16CB_12450"
     CDS             16745..17293
                     /locus_tag="AC16CB_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR3343327.1"
                     /translation="MVKPNHQPFENWLLSEGALTQEESQTLQEHLNDCETCRMLSEAL
                     RDVDRELRTASVLSPAPGFVSRWQARLEADRLRLYRRQTFFIMILSIGGAMFLLTWLA
                     IVLYPLIQSPKPVLLAVVYELTSTFTFATAIGEALMTVLRTVVGVIPPTQWAAIWTAL
                     AGLGALWFIALQRLTRPRRIIV"
     gene            17290..>17840
                     /locus_tag="AC16CB_12455"
     CDS             17290..>17840
                     /locus_tag="AC16CB_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR3343328.1"
                     /translation="MKTNLRIAILLTLVLVLILPSTAMARGLFGDRIVFGDTFTLESG
                     ETLDGNLIVFGGNAFLEVGSQVDGDVVLMGGNLEADGSIGGSVVAIGGLVSLGETSLV
                     EGDVAAIGAQLDKAEGARIIGEVINNLDTPLVFPFTDSVRVPRFEVNFSPIVNIVMFF
                     MKVLLWSALAVLVVLFMPEYTQRV"
BASE COUNT         3907 a         4530 c         5138 g         4265 t
ORIGIN      
        1 caggaggtac gcacagtaac agacaagaac ctcaaagaag ggttggctgg gttctcagtt
       61 tcgtctttca atgtcacacc cataattgtg gagtttgact attacggtgt cagtgtaccg
      121 taggtaatag ttcaatctag atgaaaaata tgtgataaga aaagcgggcg caaagacgcc
      181 cgcttttcct tgctatggtg ataatatagc tctactactc aactttgaaa gctattctcg
      241 cttctgctgt attgccagcc tggtcgacag ctttgatacg cagggagtgc gcccctaggc
      301 gagcctgcca ggtaatggtg taggggggct gggtcagatt gctgaccagt cgatcatcga
      361 tgtagaattc cacattcttt aatgctaaat cgtcgttgac ttgggcctgc aacgtaattg
      421 tctggttaga gagggggacg atctgcccat cggtggggta aagtatcgaa agttcaggtg
      481 actggttatc tactgtgacc tgtattacat cgctctgtac gcgttggtcc tgctggatga
      541 ccagcagctg cattgaatac aacccgctta gcccctgagt atcccaactg ccaagcaggt
      601 tgtctgagac tggcaactga gaatcttgac ctacctgcag ccagttcttt gggttcaatc
      661 ctcgacctac ttgcaggcga tatagttcaa aattgctgcc ccgagcggtg cccataatcc
      721 gcactttgcc ccctacgtaa gcgaacatgc tgggagaaga aattttcaca tctgctgaag
      781 gagacgtgtc agccaggatt acatcgtaag tatccggagg ggttggaaga ccagcctggc
      841 gtgcccattc atgtgcctcg ggcggcactt gcatatacac gcgctcttcc accaactcgg
      901 ggggtgtaaa tacggtagcc agcatcccgc tctctcggtt gatttgcagt ctacggaaga
      961 gggtgtcaaa gcttgtcggc tcggtgcctg caataaagat ttcgttcaca acagtgggac
     1021 aatctggggt aggcaacatc cctgatggat cacatacagc caggctattg atcccggcag
     1081 gcatggacca tccatctggc gcatggtttc gtgtggcata ttgggccact gcatgcaata
     1141 acgcagcagc ctcctgtacg gagattttcc cctgtgaagc ttgtccgagc catatgccga
     1201 tggtcatgta gggtgtagac ccaaccgccc atacatcttg ttccataccg gtgcgcccca
     1261 atttggctgc aaatggtcgt ccaatttcaa gcggattggg gtgccccaac attgaccagc
     1321 gggctggctc atcgctcaaa atgtgtgtca tcaggtaagc taactgtgga cttaccaccg
     1381 gatgtgaggc gttgttggca tgattgatcc aaaccttacc tgtggcgtct tctaggcgga
     1441 taattgtagc agctgctgtg aatggggggg tgtttcccgt agagagtgtg ttttccctgc
     1501 ccgcgagcag gccttgattt gccagaaccc catatgcacg gctgatatcc agcagagtca
     1561 cttcactttc ctggagcaag tcagtcacgc ttgcatcatc aggcagcctg gctgcctcaa
     1621 gaccgaattg cttggcaagc cgccatacgt tctccgctcc tacctgggtt gaaacctgtt
     1681 cggttggtac cagatcatcg ttcgccaagg cggttcgcag cctcagagga ccatgaaagc
     1741 ttccatccag gttgaaagat ttgtttgatc cagaagggat atcccataac attgaggctg
     1801 ggttcaagcc acgcgtgaaa ccggtcaaat agatgaaagg tgtgagcagt gagcccgcag
     1861 gatgccccgg ataatgtgcc gggtccaatc ctggagatat ttctccgatc attgccagga
     1921 tttctccggt gcgatggtct aggatgacca ggttaccctt aatatccttc acggtcatgc
     1981 tttgcccagt gaaggaggtg ggcagcagta gcgccgaccg gcattcatcg ccattcatcg
     2041 tgttgggggg atctggcatg ccctgcagtc gggctgtata caattgcaaa gcgcacgcag
     2101 cttgaaattg cagatcctga tcaagggtag tgacgatttg gaagccaccg cgctcaaggc
     2161 gattcaggct aagctcttgg cctagctgct ccaaaaccag attgataaaa atgggcgcgg
     2221 ggttggctaa ggtttctgtt tgtgtgcgaa acaccagatt gctcttgcgg gcctgagaag
     2281 cttgtgcagc gttgatccag ccgccttcca acatggtttg gatcacgtct actgctctct
     2341 ccctgaccac attaggcgca tccaacgggt tcaaagcggg tgattgcatt actgcagcca
     2401 gtacggctgc ttcagctaag ttgatttcgg ctgctggctt attaaaatag accctcgcgg
     2461 caacatctgc gccaaatgcc agccggccat agttagcgct gttgagatac cagatgagca
     2521 cctgttggcg accaaaacgc gccgtgatct gggctgccag cagacgctcc cgtactgctc
     2581 gccgtaggct ggctggctcg ctccacagca gcaaatcaga aaccagagtt tgggcgagtg
     2641 tggggtgggt gcccgaggtc aagccatcaa aagaatagcc tgcatggctc cagaattgcg
     2701 gatcggcggt tgccagtgtg gcagagatca aactcgaggg cagataattt ggctggttgc
     2761 tctgcagggt gagtacttgg cgatcaaccg cagcagggtt ttccaattct aataagacat
     2821 gtgtgccggt gcggtcatat aggcgggttg gctggagcag caacccgtct ggcgggtcta
     2881 acagggcagg cagcgtatcc agtgagggta aattatgaaa aagtgtaata tatccccaca
     2941 cccccatcat ccctaccagc accgcgaata aactaaggaa tgtactgcaa cctaccccaa
     3001 accaggtttt tgagtatcga cctgcccgat tgtgccgatg tctgcgaagg gatgtgatct
     3061 gcgctggaga gggcatactt tctcagtgcg tggtttgatt gtcaaccgcc tggagaatct
     3121 cgcgagcgga ttgcaaggtg ccctcgctta tcccacccag catctgtgcc agttcttcta
     3181 cccggagatt tccggttagt tgggaaacac gggtcattgt gcgcccttcc tgaaccagct
     3241 tctccacgtg gaagtgctga gtgccaaagg cagcaagttg tggcaggtgg gttacacaca
     3301 gcacctgatg tttttgtgcc aaccgccaaa gtttctggcc gaccactgcg ccgacacgcc
     3361 cgccgatgcc ttggtcaatt tcatcaaaga tgagggtggg gatttgatcg gcgctggtaa
     3421 gaacatgttt aagggctagc atcaggcgtg aggtttcgcc tccggaggcg atctttacca
     3481 ggggtttcaa accctcgcct gggttggggg cgatgagaaa ttctgcctgc tccagcccgc
     3541 tcgagtcgaa cgctactcgt tgtccatcac ggaggggtgc gccgtttggg tcgggtcggc
     3601 tccgaaattc cacctcgaac ctagtccccg acatttgcag gtcgtagagc tcattttcca
     3661 gggaccgccc cagcttctca gcttgctccc gacgcttttc agagagcata cttccctgta
     3721 tgccaatttg ggaaagcaat ttggcctcat caaattcaag ctcctggatg cgctcgcttg
     3781 catgggtaat ggcgtctaac tgctggcgag ccctctcagc gtattgaagt attgcttcga
     3841 tgctctcgcc atatttgcgt tttaagtttt ggagcaaccc aaggcgctct tccacctggt
     3901 cgagccgctt tgggttgaat tcaatacctt ctaagtaaga gcgaatttcc ctgaccatct
     3961 ctgagaggct atcgaataag agctgacctt gattgctcag attatcttgt gatggatcca
     4021 gcttggcaag attactcaag gcgtggacag cttgcccaaa caggtcggta acggctggtg
     4081 tttcagatgt gccttcatct aaagcttgca acgcctcatt tgcgagtgaa gccagccctt
     4141 cggcattcgc taggcgattg cgctcatctc gcagtgtctc ttcttcgcct gggcggaaat
     4201 gggctgcttc gatttcgttg atctggtagt tcagcgtgtc gatgcggcgg gcggcttcct
     4261 gttcagcttg gcgtagctcg cccagctcgc gccgcacagc ttgcagcttg tggtaggtct
     4321 tctggtaacc tcccagcagg tcttccaagc ccgcgtaatt atccagcagt ccgaggtgct
     4381 ggcggacacg cagcaaggat aagtgctcag actgtccatg aacatcgacc agatactcgc
     4441 cgatctcgcg cagtagtgtg gcgttgacgc tgcgcccatt gagacgggca atgttgcgct
     4501 cgttgaggcg aatttcccgc ccgagggtca catattctgg gtcgtcaagc agctcttcgc
     4561 gctttaaaat agaatgtact ggctcacgca caggggggga aatatagaat ctgccttcta
     4621 cataggcgcg ctcgcaaccg ctgcggacca ttgtggcatc tgctcgacca ccaagcaagg
     4681 tttccacggc atcgatcagg atggatttgc ctgcgccagt ctcgccagtg aaagtgatca
     4741 gacctgcgcc aagctgcaga tcgatcttat caataatcgc gaagttctct atgtgtaatt
     4801 ctttgagcat actcgcgcaa cagccccatc agacgcgcat ttggatgccg cctaaacggt
     4861 agtatacggt cgatgtcaca ataaaggtgt agactccttg ccaaaggaca ctccatagtc
     4921 ctcccaggtt gccttgtaat aagatcaaga ggccgatcag caggaatggt gagcttccca
     4981 ccgctgtagc gattgccgcg atctgaaaaa gaagcttgga acggcggcgc tgtatagcaa
     5041 agcgaaccac ctcggcgata atcactccag cgactggggc cagcaaaatc gtgaaaaacc
     5101 caaaactcgt gataatgcgg ctacctacca gggaaagcag acctgctaag atcgtagcag
     5161 acacatagtc ataccagcgg ctggtttcga acaacttttg ctggtttcgc acgcactctg
     5221 cgcagcgata gccagtgggt gtggagatag cacactttgg acagatcggt ttgctgcaac
     5281 gattgcagcg aaggttggtc tcggtttggg ggtgattttc acaataaagg gtggcgggga
     5341 tatcagtcat cgttgattac ctgtcgttgg gttttgctcc atgtgagggg ttagatttcg
     5401 ataaaaatag cctggatctt ggaagcgaac gaattttaac gtatagtcac tggcgcgggc
     5461 gatgaccttg tcgccgtctt gcacgttgat cggcgtttgt ccatcaaccg aaaggaccgc
     5521 gtggtgcgtg ctgtgtatcg tgataccaac agacgatcct tctgccagga caatcgctcg
     5581 atcaatcgat aggtgcggtg caacagcgac cagtaatatg ttgcgcaact cggggggcaa
     5641 gatggggccg cccgctgcca gcgcgtacgc ggtggagcca gtgggagtag cagcgatcag
     5701 ggcatcggcg acatacgtgg tcaggtagcg cccgtctacc tctgccacga gctgcactgg
     5761 ccttacggtc tggcctcgtc caaccacgac ttcgttgagc acgtcccatg tgccgaggat
     5821 ttcatcgctg cgatatagct cggcattcag catcatgcgt tgctctaacc agtaatcgcc
     5881 ggtcaataag cggggcagaa tatccatcca ctggttctgg cggacctcca taagaaatcc
     5941 aaagtgtccc agattgatgc ccaatatcgg cagacccaaa gggccgcaaa gatgcccagc
     6001 gcgcaacatg gtgccatctc cccctagggc aatgagcagt tcaaatgcac cagttttaat
     6061 ttgggttctc agctcatcgt cataaagaag cccataagag acctgaacat ctttttgctt
     6121 taaaaaagtc gcaattcgtt cggcctcaag ataggcatca tctaattcgg gatgagcaac
     6181 aacggcaatg cgccgcagct gtggcattga acctggcata tcgaatgaat tataccttct
     6241 tagcttagcc tcacatcgca gatggagcgg aatccacaac ttcggcagcg ggctggggat
     6301 tcatgggtgc ggggcaattc atgcaacttc tcttgatcac gcatctcttc gaggagagct
     6361 aaggtcgagg attcgagctt gctggtgtat tcgatctggt atgtccgatc gggataatgc
     6421 aggattccgt atggcgggcg tacgccaaaa gtgcgctcga tcagcagaca ataggtcgcc
     6481 agttggaaga tgtgggaatc atatggggat tgcgccgcgc gtgtggattt gacctccacg
     6541 gggataatct ttttcttgtg tttaatgagg tagtccggct tgccagttag tctaagatcg
     6601 gggtcgtaga gaggctctgg tgttggcgac cagctgcgtg tgtcggcata gatgatcttt
     6661 ccaggcggaa gcccggcagc acgttgacgc ctgttgacca accagagcag taccaaggcg
     6721 agcagggcag cgagcgcagc gataggcaac atatcgttgt ttatgatgaa aactgcagaa
     6781 taagggatac gatcgccaat atcccagctg cgaggagtag gaaataggcc aatttccgca
     6841 gagtatctgc cttctgaacc ttgctcccat gctgatcgtg ggcgtggatg cctgcagcca
     6901 gttctgcctg gttttgcggt tcgtagccgc ttttccggta ccaccaggca cgctggcagt
     6961 acaggtaggt gccaatttca gatgagcgta tgatcgtcat gtgcttatag atgtggggtt
     7021 gctgattttg catcccgaag cttaagattc gaaccgcttt tccagtcgtt tgaggctttg
     7081 ttctaggtgt tcctggcgca agctgagggt catgtcgccg cgtgtgcgct cgttcacgcc
     7141 gcgcacaata tcggtcaggc ggcgcaatcc acccgtgatg gcatcaggga aatccatggt
     7201 aaccagcaca gcgtaaatat catccatgtg actgagcagg cgctcggctt cttcggaatg
     7261 gccatgcctg aggatatcga ggcagcggcg gcgcagttca ccggcggctt ctgcgagacc
     7321 ctgcaggtaa gtggcatatt ctaatcggag ttcgtcgggg ctagggagag gctgatcata
     7381 gaccagggca aacactgaat tcgcctctgc aaattccttg agcgcatctt gcgtatagcc
     7441 tgtgtagtat aagtctggaa agtcagccag atctttgtgg atgctgttgg cgagatctcg
     7501 cgccgcgccg aggtgctcct gtgcattggc gcgttcgtct cggtggatgg cgcgaattgc
     7561 gttggcgcaa tggcgcgtga gcgtacgtgc ccgggtgaga gcctgatcgc gcaccagtgt
     7621 gcgcgtttcg aaggattggc ggatgcgctc ggcaatatca tcaaggtttt gcatagatct
     7681 tacctctggg gagggttttc ggggggttgg ggcgggcgga agagttggtc agcaagggtt
     7741 gatccactgg gaattttgat ctctccgtag gctgattcgt attttcccag gttatctgcc
     7801 agtgcgcgga aaaacaattt tgcacctagg gcggacataa cgatgcggga ttgcaggcga
     7861 actggtgtgc cgcctggcag tagctgggcg aaatcaaaaa caatctctga gggagagtga
     7921 gcgatgcgca ccaggttggc atactcgata gggaaatcgg gcggcacttc caacggaggc
     7981 aaattaggtg ccgccggtcg tgggggagaa gatgaaggca tgcgtatctc ctggaattag
     8041 tttccaccac cctttatttc ccattccatt atgcgggtaa aggatttgtt ggattttggg
     8101 cgggagcaac ccgcccaaaa tccaaattat cagcttatcc aattcccgca atgcagggga
     8161 atgggttagg ggttggggat ttggtgatca atcagaaccc gaaaaaatcc attcggggtt
     8221 tgtcatacta gaaggggata tcgtcatcgt tggctgaaac ttccccttcg ccgaagggga
     8281 tttcgccttc ggcctcgcca cgggtggaga ggaagcgcac tgtgttcgcg ctgacctcaa
     8341 aggacgctgc agcggtccca tcctggcgcg tccagacccg aggccctcca ctggcctgat
     8401 ccggtattag gcggccctca accaatactt tgctgccttt gcgcaagtat tggttgcaca
     8461 cctctgctgt tttaccccaa gtggagacac ggaaccaaat ggtttcctta acaggagtac
     8521 catccgaagc cgtatattgc cggttggtgg caatgttgaa gttggtgacc gctgaaccgc
     8581 caggtgtata acgcatctca gggtctcgac ctagatttcc aacgacgata atggtgtgat
     8641 acattttggt atctcctttc gttcaggtga gcggtctgtc ttaagttccg gacgcacacc
     8701 gcccggggca ggccctagat tcactcaccg aataagcaga acaattgtac actaaaaaca
     8761 aaaaacgcgt taggtatttt tttgcgagag ttcaggattt tgactatcat gcgttagaat
     8821 tgatccaggc gaagaattgc gggagggagt gcaactctga gatatgtaaa aattttgcag
     8881 gagtttgcac gggcaagtca tcccaggtgc gatcagtggg ggaaagtcgg cgagctttgc
     8941 tgtggaaatc tcccaccgtc atgtagccag ccaatatgat ctcgaccccg ttaaaccaca
     9001 ggttaaccca acgatgagct ggaatgcgca aaggtcgatc tggttctgct cgtgacaaag
     9061 agagttgtac tgccgggatt gtctctgagg acagatatgc cagggctgtg taattctgag
     9121 caacgttcac gctccggtgt gcaggcaagc aaagatgctc gctctggtat tggcctttaa
     9181 cccctcgacc accaagctca atgacgaatt cactttcacc ttcattggtc agggatattt
     9241 gaccatatac tctcccaggc gcaggatttt tccaggcttt tggcaaaatg tacgcaaaat
     9301 ggcaaggaag aatcttttct ggatagcggc ggatttcata ccagctaggc tgaggctttg
     9361 ctagtatgat gatgaaaacc agcaggtcat gatgctctag caactcagag gttagataca
     9421 cgtctgttag gcaagctggg gtttgcaaga tgcttgaatg ctgatctttt atatggcgca
     9481 ggattgatct gcgggagtac aggatttcta caagatggca gcggcgcccg cccagtacga
     9541 catcacccca atcggattgc gtaaagggtg tggtttggag ggttttaaag ggaatccttg
     9601 cttctgtcag gtgtcgtcgc aaggccagtt cccccgcaac agccgtgacc agcctgcgca
     9661 agggggcgat gtgcaggttg tccgcctgaa cgagaccgag cgccaggctg cgacgcgcct
     9721 gcaagacgcc ggctgatgtc aggcctgggg tatagggcag gtgtatgaga tcttcagcag
     9781 tgatcatgat gcagagatcc agcggactaa ttgtctaaga acttgcccct atcccttatc
     9841 acttatctcc aaatcatttc taaattgaag gcagtagtgg tggaaggtgt tgctcaccca
     9901 ggcgcttccc agaccgtatg gagctaaacg ctggttgtct tggcaccaga tgcgttcgtc
     9961 tttgagcgtg tagaactgtc ccaacaggcg acctaaatcc caggctaaag ggtaaatcac
    10021 tccacccttc ttgacgttct tgacgatgat ggtcagatag gcgccttgct tgacgaatgg
    10081 cttgagcgca gcgtagatat ctactagccg gcgcaagaac tcgtcatagt cgctcagatt
    10141 gcccaggtca gcagggtcat cggagtagaa tacatctagc tcgggggagg cgcggcgatt
    10201 tttctgtgtc tgcgcgccgc gagcgcgtag catgtcccaa taaggtgggg aggtcaggac
    10261 ataatctgct gggggaattt ggagagtgtc ggcatagtga gcaatatctg cggcgttgcc
    10321 cacgataacc tcggcggtca gatctcttgc ctgctcaccc agcaatgcct gctcttcgct
    10381 gaccacttgc tgagcgatgg cagcataggc gggattcagc tcgacgccgt agctgtgcct
    10441 gccgctgcgc agggcagcta ccatggcgct acccgtgccc accatggggt cgaggaccac
    10501 ctcaccgcgc cgcgtgaaga actcaatgaa ctcttgcgcc aatgtctccg gaaacttggc
    10561 cgggtggcgc aacacgtcct tgcggcgcgg tggcgggttg tgaacaaacc atgacttttg
    10621 aaacttgagc cattccttgg ggctaaggtc gttcagacgg ttaacgagcc gccgctttgc
    10681 catgctacct ccttagttgg ggattgtccg caccgataaa ttggtaaagc tgactgtcaa
    10741 tgcggtatca cctgctgagc gcacaaagaa acccaggctg ccttcctgga gcgaagggtc
    10801 tctgacagta aattgatatt cattgttgac gtaaaactgc atctctttgc cctttaccca
    10861 cgcgctgatc agagagctgc ttggcgcgcc ccgcgggaca gagccactgg gggtttttgc
    10921 ttggggggaa gatgctttcc cgttgtaata cttgtctagg cgtatgcttc cttcgcagga
    10981 gagggagaaa cggtagaatt ctgctgaagg ggagacgcgc agcagcaagc cgtattcatc
    11041 atcgcctcgg cacaggctgg ggcttgccgt gacttctaga taaaaatccg ataggatggg
    11101 gcttttcctc agtgtgtaca ggtatccact cggttggttg agcgccaggg ttagagcttg
    11161 attacccagg gcgatgctgg tggtggaagt ctgccccaat aaccactcgt ccggatcgtt
    11221 gaagtcgtcg ctgaagatca gatcgcctgt tggcggaagc tggtcaacag tgggtgatac
    11281 atccaaggtt gggagcggcg tctgggtggc tgtggcggga aaccaaatta ccgtggcggt
    11341 tggtgttccg gttggagtcg ccgtgctggt cggctcgggt ggaagtgctg tggggcgcga
    11401 caggcacccc gccagaatca tggacaggac gagccagagt ttatatttca acattcaacc
    11461 cttagctgat ctcgaccact tgctgacgct ctggtccaac ggaaaccgtg cgcaccggca
    11521 cgctgctcaa ctcctcgatg cgcaggatgt aagctcgtga ctggggaggt agttcttccc
    11581 aggtgcgtgt ggagcgcaag tcattctgcc agccgggtag ctcttcgtat accggttcaa
    11641 agcccgccag ttcaccaggt cctaagggta gatccttgta tacctgtcct cccttgcggt
    11701 aagcagtgca aatgcgtagc ggctccaagc cggtgaggat atctagcttg gtcagtgcta
    11761 gctcggtaag gccattaatg cgcaacgcgt agcgcagcag caccatatcc agccagccta
    11821 cccgccgcgg tctgccggtg gtagtgccat attcgtccca gggctgttcc ccggtgccgc
    11881 gcaagtgcaa ggccaggtcg ccggagagct ctgtggggaa tggacctgca cccacgcgtg
    11941 tttggaaagc tttagtaacc ccaatgacgc gctcgacgaa gccaaccccc aggccaagcc
    12001 ccagcaaggc gccagctgcg gagggatacg aactggtgac aaatggatat gacccgtgat
    12061 ccaggtcgag gagggtgcct tgggcgcctt ccgccaggac acgctgacca tccttcagtg
    12121 ctttggagag cagcgcagac acgtcttgaa tgtagggctg aagttgttgg gtaaattgga
    12181 tatattcgtg tgtcgtagcg gcaacatcca gtggttcagc atgatatagt tgggtcaggc
    12241 gctggttagc tgcttcaatg tgagcctgca aagctggaac aaaagagcgg ctatccaaca
    12301 cgtcacccag gcgcagcccg ctgcgcgcca ctttatcagt ataagctgga ccgatgccgc
    12361 gcaaggtagt accaattgcg cctttgccac gcgccgcttc ttgagcagcg tcgatagcac
    12421 ggtgggcagg tgtaatcagg tgggcagcgt atgacaggcg cagccgttcg ggatcaatcc
    12481 gcacaccagc ctctcggagc atattcatct catccaatag tgtggctggg ttgatcgcca
    12541 caccattacc gatcacaccc acagtatgcg ggtggatcac accggatggg atcaggtgta
    12601 gcttgaaagt ctgatctgcc accgttaccg tgtgtccggc gttgtctcct ccattgtatc
    12661 gggccacgta gtctgcatgt gctgagagca ggtctacgat acggcctttg ccttcatcgc
    12721 cccattgggt tccgacgata atatctaaag gcatgctcgc ctccagagat agttgttgta
    12781 aaatagttct gtaatttcta tcctctcaat ctatcgtgga gtagggatac cccatcctcc
    12841 actccattga gaaggtactc taatttcatt ctagcataga aacaaccgtt gaggagaggc
    12901 tgatgcgcaa aaaagtgatt atgatcaccg gtgcggctgg cgaaattggg aatgcattaa
    12961 tcaccagcct tgccgaacag agcaatgttc cgatcttatc cctggacttg caacctttgc
    13021 ctgagaaaca agcccgcatg acgactcacc ttcaaggaga cgtactggat caagctctgc
    13081 tttcacgcct gatcagtgag tacgagatcg acacgattta ccaccttgca gcgttgcttt
    13141 ccacccgctc tgagtttacc cccgaggctg cccaccgtgt taacgtggag ggtacgctgg
    13201 tgctattgag gctggcaacc gagcagtccg catggcgagg cacgccggtg aaattcattt
    13261 ttccaagctc tattgctgcc tatggcatgc cggatctggc gaccaaaaaa gcctacgccc
    13321 gggtgcgcga gtgggagtgg aactaccccc ggacgatgta tggctgcaac aagctctact
    13381 gcgagcaatt gggcatttat ttcagccggc actaccgcca gctggcagct gaccgatctg
    13441 tgatgctgga ttttcgcagc ttgcgttttc ctggattgat cagcgccttc actgcgccca
    13501 gcggtggcac cagtgattat ggtcccgaga tgctccacgc ggctgccaaa aaggttccct
    13561 atgcctgctt tgtgcgcgaa gatgtgcaga tcccgttcat ggcaatgccg gatgcggtga
    13621 cagcgctcct tcgccttacc gagtctccgc atgaggcttt gagccgcttg gtgtttaatg
    13681 tgaccagctt cagcctgacc gccgccgagt ttcgcgagtg ggtattgaag tatttccctg
    13741 acgcccagat ttcgtttgat cccgatgtga agcgtcaggg catcgtggat agctggcctg
    13801 ctgacctgga tgatagtgcc gcccgccgag attggggttg gcaacctgat tatgacctgg
    13861 agcgctcttt cagcgagtat ctggtgccaa atatcatgaa gcgttacagc gcttgatctc
    13921 actatcgagc aaccaatcgt gcacagggta gaataattaa gcgaatttat tgattagaga
    13981 tagagatatg gatataacag attaccctga agtaaaacct ggcccgatcg tactcttcgg
    14041 ctcgggcgag accgctccta gcgggcgtaa aattttcgat catgtcatgc gccagttgcc
    14101 gcccaaacca aaactggctt tactggagac cccggcaggg tttgagctca actcgtctca
    14161 ggtaattggg cgggtgggtg aatttatctc tcaccgcctg caaaactatg accctcaaat
    14221 cactatcgtg cctgcacgta agcgcggcac agcattcagc ccggacgatc ctgatttgat
    14281 cgaaccactc tatgaagctg atatgatttt catgggtccg ggtagcccta cttatgctgt
    14341 ccggcagttg agaggtagcc tggcttggca tgccctgctc gcccggcacc gccttggggc
    14401 gaccattgtc tttgccagcg cggctaccgt ggctgccagc ctgttttcta taccagtgta
    14461 cgagatttac aaggtcggcg aagagctgca ttggaaggat gggctggatt tttttggcct
    14521 gtacgggcta ccattggttt tcgtccccca ttggaacaac aacgatggag gagaagagct
    14581 ggataccagc cgctgcttca tgggcaagcc acgcttcgct gagttgatgg aaatgattcc
    14641 ctcagagctg tccatactgg gaatagatga gaagactgcc ctggttatgg atccgcagtc
    14701 gtgcgcgtgc cgggtgatcg ggcttggcgg ggtgacgatc atccatgccg gcccggcgca
    14761 tcgcagtgca acccaaccca gccagctgga tggtaccggc ttgaacgagg ttgccgaaaa
    14821 tcgcaaggcg cacgttcacc attaccagca tggtcaggcc ttttctttga aaaggattgg
    14881 cccgttctgt gatccagaag ctggcgcggg gctacctggc gaggtctggc aacgcgccct
    14941 ggaagtccac catgcgcggc agagccagga aatggcttcg tttgaagatg agtcaccccc
    15001 tcctgaggtg ctcgtcctgg tggaagagcg ccaggctgct cgggcgcgca aggattgggc
    15061 tgcctccgat agcttacgcg aacaggtggc tgacctgggc tggctgatcc aggatacgcc
    15121 ggaggggccg aaggtggtca aaagcgacga ttaatttccg aatttttgat gacaataagg
    15181 agtcactatg acgcaaacgc tttccgttgg ttacatcggc ctagggctga tgggtaaatc
    15241 cattgcccgc aatatcctta aagcaggttt cccgctggtg gtgcataacc gaagtcaggc
    15301 agcagtcgaa gagctggtcg ctgaaggggc ggtgcgggct ggctcggcgg cggaggtagc
    15361 gtctagtgtg gacgtggttt tcaccaatct gccggatacc cctgacgtag agaaagtggc
    15421 tttgggttca aatggggtca ttgagggtgc acatccgggg ctggtcttcg tggataactc
    15481 cacgattcag ccggctgcgg cgcggcggat tgccgaggta cttggtgaaa aaggggtgca
    15541 gtgccttgac gctccggtga gcggcgggga cattggcgct cgccagggga cgttggcgat
    15601 tatggtggga gggccagcgg aggctctgga tagggtcatg ccaatcttcg aggcgatggg
    15661 caagaccatc actcatgtgg gtggggctgg cacaggccag atcgctaagg ctgccaacca
    15721 gatcatggtg gctgcccaga tggtggcgat gggcgaattg cttgtttttg cccaaaaagc
    15781 tggcgcggac ccgcgcaaag tcgtggaggc catccagggt ggtgcagctc agtgctggac
    15841 gctggatgtc aaaccgcagc gcttgtttgc cggcaaccga cagccgggct ttaaggcata
    15901 catgcaggcc aaagatttgg gcatcgtgat ggaatccgcc cgccaatatg gcataccttt
    15961 gcctgcagcg gctgtgcaca cgcagctctt caatgccatg ctgcagatgg gtatgtctga
    16021 gctggataac tcggcggtat tggctgtgat cgaacaactc gctgggatcc gtctttcgga
    16081 ggggtaaatt attttgcaac ctgaagttgt aatctgcgta tatacagttg aatagttcag
    16141 gcgcggaatg cgcgccacag gagcattgca gtgaatgata ccgagtcaag ctggctcgcc
    16201 caggctcaga aaggcgatgc agaagccttt agccgcttga tcgagaccta ccagaggcca
    16261 gtttataacc tgtgttaccg catgttgaat gacgcaggcg atgccgagga tgctgcgcaa
    16321 gagacattct tgcgagcata caaatcgatg aaaagatatg atcaaagccg tcctttccct
    16381 acctggttgc tttccattgc tgcgcattac tgcatcgatc agattcgcaa gcgcaggatg
    16441 gcgattgtat ctaccgagga cttgccctac ccggatctat cggacgatgc cccaggtccg
    16501 gaatctgtgg tgagccgcaa agaggagcag caacgggtgc gtgtcctgct ggacaccttg
    16561 agccccacgg atcgtgctgc tgtagttatg tattactggt atgacttttc ctatgaagag
    16621 attggtgaag cattatcgct cacgctcagc gccgtcaaga gtcgcctgca tcgcgctcgg
    16681 ttgagcatgg cacaggaatg gatcgaccag caaccacagg ccggtttggc cgaaaggaag
    16741 caacatggta aagcccaatc accagccttt tgaaaattgg ttgctctctg aaggggcgtt
    16801 aacccaagag gagtcacaaa ccttgcagga gcatctaaat gattgtgaga cctgccgaat
    16861 gctttcggag gcactccggg atgtcgatcg tgagctgcgg actgcgtcgg ttttgtcgcc
    16921 tgctccaggt tttgtaagcc gctggcaggc gcgtttggag gcagatcgcc tccgcttata
    16981 ccgcaggcaa acctttttta tcatgatatt gagcattggc ggggcgatgt ttttgttgac
    17041 ctggttggcc atcgtgctct acccgcttat tcaatctccc aagccggttt tgctggctgt
    17101 ggtttatgag cttacttcta catttacatt cgccaccgcg attggggagg cgctcatgac
    17161 ggtgctacgc acggtggtcg gggttattcc gcccacacag tgggcagcca tatggacagc
    17221 gcttgcgggt ttgggcgcgc tttggtttat tgcattacaa cggctaacaa ggccgcggag
    17281 gataattgta tgaaaacaaa cttacgtatc gcaatattgc ttaccctggt tttggtcctc
    17341 attttgccga gcacggcgat ggcacgcggg ttgttcggtg acaggatcgt atttggagat
    17401 acattcaccc ttgagagtgg tgaaacgctg gatggaaacc tgattgtctt tgggggtaat
    17461 gccttcctgg aggtaggttc ccaggttgat ggtgatgtgg ttttgatggg aggcaacctg
    17521 gaggctgatg gcagcattgg cgggtcagtg gttgctatcg gtgggctggt ctccttggga
    17581 gaaacgtctc tggtggaagg ggatgtcgcg gccattggcg cccaattgga taaagccgag
    17641 ggggcgcgta ttatcggtga agtgatcaac aacctggaca caccgcttgt ctttccattc
    17701 accgacagtg ttcgtgtccc ccgctttgag gtgaatttct ctcccatagt aaacatcgtg
    17761 atgtttttta tgaaagtctt gctctggtca gctctggcgg tattggtggt tttgttcatg
    17821 ccggagtaca cccagcgtgt
//
LOCUS       DBNGSX010000422         4509 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_454600, whole genome shotgun sequence.
ACCESSION   DBNGSX010000422 DBNGSX010000000
VERSION     DBNGSX010000422.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_454600"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /gene="rplT"
                     /locus_tag="AC16CB_12460"
     CDS             complement(<1..332)
                     /gene="rplT"
                     /locus_tag="AC16CB_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIR3343329.1"
                     /translation="MARVKTGPYRRKHHKKILKITKGQFGTRSRLFRRANEAMLKSFW
                     YAYRDRRTRRRELRRLWIARINAAARMNGSTYSRLIHDMKAANVAINRKMLSDLAVRD
                     PNAFAAVV"
     gene            complement(452..679)
                     /locus_tag="AC16CB_12465"
     CDS             complement(452..679)
                     /locus_tag="AC16CB_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large ribosomal subunit protein bL35"
                     /protein_id="HIR3343330.1"
                     /translation="MPRKQTTGKYKIKTHKATSKRFRLTGSGMLVRTKGGKGHLRRRT
                     SKRTKRLFTEMIPVKGRGIKKRVRRLAPYQK"
     gene            complement(794..1321)
                     /gene="infC"
                     /locus_tag="AC16CB_12470"
     CDS             complement(794..1321)
                     /gene="infC"
                     /locus_tag="AC16CB_12470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIR3343331.1"
                     /translation="MSAANYRVNESIRVPEVRLIGPEGENIGVVSIDEALRIARDADL
                     DLVEVAPAADPPVCRVMDFGKFIYERAKKEREARKAQTKIEIKEIRLRPKTNEHHRDL
                     KVRDARRWLEAGMKVKVRIRFRGREISYPEIALSDLKEIAEDLSDLAQIEQAPALEGR
                     TMLMVLAPGKGAKKK"
     gene            complement(1440..3236)
                     /gene="thrS"
                     /locus_tag="AC16CB_12475"
     CDS             complement(1440..3236)
                     /gene="thrS"
                     /locus_tag="AC16CB_12475"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR3343332.1"
                     /translation="MAEKKQERYEDSDLYCIRHSAAHVMAQAVVEKFPEAKYTIGPPV
                     EDGFYYDFDLPRSLTPDDLQEIEQRMREIIAGDHPFVKKVVSADEARQIFEQQPYKLE
                     LIEGLEQGGFDEYGQPLAEKPEISIYTHDTFVDLCRGPHVENTRKINPVAVKLMSVAG
                     AYWRGDEHNRMLQRVYGTAWRDAQELEEYLWRIEEAKKRDHRKLGKELGLFYFSDDVG
                     PGLPLFTPKGETLRYLMENFVRDVQTRFGYQHVWTGHLVKEDLYKRSGHYDNYKESMF
                     PPMQDENVVFRLKPMNCPSHMTLFREMGRHSYRELPLRFSEFATLYRYEKTGELTGLT
                     RVRALTQDDCHIFCTEEQVGSEFSLALNLIQEVLARYKFTDYVVRLSLRGEGGKYVQD
                     EEKWVKAEAALREALDASGVEYFEATGEAAFYGPKADFLARDVLGREWQLSTIQVDFI
                     QPARLGLSYIGEDGQEHTPVVLHRAVTGSTERFLGVVIEHFAGAFPVWLAPVQAVLIP
                     IADRHLSYAHQVAEKLKAAGVRVEVDERSERMNAKIRDAQNQKIPYMLVVGDREMEQG
                     AVALRLRSGENPGAISLEEFLERVNEDLREGS"
     gene            complement(3524..3597)
                     /locus_tag="AC16CB_12480"
     tRNA            complement(3524..3597)
                     /locus_tag="AC16CB_12480"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3561..3563),aa:Val,seq:gac)
     gene            complement(3650..4054)
                     /locus_tag="AC16CB_12485"
     CDS             complement(3650..4054)
                     /locus_tag="AC16CB_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343333.1"
                     /translation="MKVPNFSWRYALIIIGFVVLAILVMDFNNRMSALRRLSTRKEGV
                     LAQLEGQIQTQDSLQTQIAYATSDASVIEWAYEEGSMVRPGDVPVVPLAQVAGTPVPT
                     PTPVVVSTPIANWQFWLWLFVDANEPASARAP"
     gene            complement(4157..4240)
                     /locus_tag="AC16CB_12490"
     tRNA            complement(4157..4240)
                     /locus_tag="AC16CB_12490"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4204..4206),aa:Leu,seq:cag)
     gene            4375..>4509
                     /locus_tag="AC16CB_12495"
     CDS             4375..>4509
                     /locus_tag="AC16CB_12495"
                     /inference="COORDINATES: protein motif:HMM:NF024776.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3343334.1"
                     /translation="MKEQHKLKMVIDYEAGKIRAQRAAELLGITKRQFRRLVAAYRQR
                     G"
BASE COUNT         1031 a         1292 c         1083 g         1103 t
ORIGIN      
        1 ctcacgaccg cagcgaaggc attggggtcg cgaaccgcca gatctgagag catcttgcgg
       61 ttgatcgcta cattggcggc cttcatgtcg tggatcaatc gactgtaagt ggagccattc
      121 atgcgtgccg cggcgttaat gcgcgcaatc cacaacctgc gcagttcgcg cctgcgggta
      181 cgccgatcgc gatacgcgta ccaaaagctc ttcagcatag cctcattagc acgccgaaaa
      241 aggcgcgagc gcgtgccgaa ttgacctttg gtgattttta agattttttt atgatgcttg
      301 cgacggtacg gacctgtctt tacacgagcc attctatact ctcctgcgaa cccctaggcg
      361 ccggtgcttc attaatagca cccgttgctt acacctaaca gccgcaatca aaagatgata
      421 tttctgcgag cctccacctt acccattaat attacttctg atatggggca aggcgacgca
      481 cgcgtttttt aatcccacga cctttcacgg ggatcatttc ggtgaacagg cgtttggtgc
      541 gctttgatgt tcggcggcgc aggtggccct taccgccttt agttcgcacc aacattcccg
      601 acccagtcag gcgaaagcgt ttggaggtcg ccttgtgggt tttgatttta tattttcctg
      661 tcgtttgctt gcgaggcacg tccgaactcc tctcacaaaa aattccgcgg ttttcgggcg
      721 ggatgaccgc catctctacg atccgcggat cgtctgactc aacattattt atatggacta
      781 caactcagcg gaattacttc ttctttgcac ccttgcccgg agcgagcacc atcaacatgg
      841 tacgcccttc taatgctggg gcttgttcaa tctgcgccaa atctgaaaga tcttcggcga
      901 tttctttcag gtcggagaga gcaatttcag gataactgat ttcccggccg cggaagcgaa
      961 tgcgaacttt tactttcata ccggcttcca accagcgccg tgcatcacgg accttcaggt
     1021 cgcggtggtg ctcgttcgtc tttggcctta ggcgaatctc cttaatctca atcttcgtct
     1081 gggcttttcg cgcctcccgt tccttctttg ctctttcata gatgaacttt ccaaaatcca
     1141 tcaccctgca aacaggagga tcggctgctg gggcaacttc caccaggtct aaatcggcat
     1201 cacgcgctat ccttagagct tcatcgattg atactacacc gatattctca ccttcgggcc
     1261 caatcaggcg tacctcaggg acacggatgg attcatttac acggtaattc gctgcgctga
     1321 tatgtcactc cttctcaaat gctgaaacaa atcactggcg caaagccagt gtgactcatg
     1381 ataccatgaa tcggcgaaaa tcgtcaacag aatttcgagt atatccaggg agataaaatc
     1441 taacttcctt ctctcaaatc ctcgttcact cgctctaaaa attcttccag tgatatcgct
     1501 cccgggtttt cgcccgagcg caagcgcaag gcaaccgcac cttgctccat ctcccggtcg
     1561 cctaccacaa gcatgtaggg gattttttga ttctgcgcat cacgtatttt ggcgttcata
     1621 cgttcgctgc gctcgtccac ttcgacacga acacccgcag ctttcagttt ctcggcaacc
     1681 tggtgagcat atgataagtg acggtcagca atcgggataa gaacagcctg tactggtgcc
     1741 agccaaaccg ggaatgcccc tgcaaagtgc tcgatgacga cacccaaaaa gcgttccgtg
     1801 gagcctgtaa cggcgcggtg caacacgacc ggtgtgtgct cctgaccgtc ttctccgata
     1861 taacttaacc ccagccgcgc tggctggatg aaatccacct ggatcgtaga tagctgccat
     1921 tcgcggccca atacatcccg cgccaggaag tcggcttttg gaccatagaa agccgcttcg
     1981 ccggtggctt caaagtactc cacaccgctg gcatccaggg cctcacgcaa agccgcctcg
     2041 gctttaaccc acttttcctc atcttggaca tatttcccgc cctcgccgcg cagcgacaat
     2101 cgaaccacat aatcggtaaa tttatagcgg gccagcactt cctggatcag atttagagcc
     2161 agagaaaatt cactgccgac ctgttcctcg gtacaaaaga tatggcagtc atcctgcgta
     2221 agggcgcgca cacgcgtcaa cccagtcagc tccccggtct tctcgtaacg atagagtgtg
     2281 gcaaattccg aaaagcgcag cggcagctcg cggtaggaat gccttcccat ttcacggaat
     2341 agcgtcatgt ggctgggaca gttcatcggc ttgaggcgaa acaccacgtt ctcatcttgc
     2401 atgggcggga acatcgactc tttgtagtta tcgtaatgcc cggaacgttt atatagatct
     2461 tctttcacca ggtgcccggt ccatacatgc tggtaaccaa agcgcgtctg cacatcgcgc
     2521 acgaagtttt ccatcaggta gcgcagggtc tcacccttcg gcgtaaagag cggcaatccc
     2581 ggtccgacat cgtcagaaaa ataaaacaaa cccaactctt tgcccaattt gcggtgatcg
     2641 cgcttttttg cctcttcaat acgccagagg tattcttcca attcctgtgc gtcgcgccat
     2701 gctgtaccgt aaactcgctg caacatacga ttgtgctcgt caccccgcca gtaagctcct
     2761 gccacgctca tcagcttaac cgcaactgga ttgatcttgc gagtgttttc tacatgggga
     2821 ccgcggcaga ggtctacgaa ggtatcatgt gtataaattg aaatctccgg cttctcggcc
     2881 agtggctgcc catactcatc gaaaccgccc tgctccagcc cttcgattag ctcaagctta
     2941 tagggttgct gttcgaatat ctggcgcgct tcatcagcac tcaccacctt tttaacaaaa
     3001 ggatgatcgc cagcgatgat ctctcgcatg cgttgttcga tttcctgcaa atcatcgggg
     3061 gtcagggagc ggggcagatc gaaatcataa taaaagccat cttccaccgg agggccaatc
     3121 gtatattttg cctcggggaa tttctccacc acagcttggg ccatgacatg tgctgccgaa
     3181 tggcgtatgc aataaaggtc gctatcttcg tagcgctcct gtttcttttc agccatatta
     3241 atctcctttt ctttccctta acaaaaaacc tcgcccttct ggggcgagag gtgctattca
     3301 cgcggttcca cccagatttt agcccaactg tcctttaagg aacagtcctg ccaatctctt
     3361 tgaaccgata acggggtcaa ccgtttacct tactcagctc acgcttttgg ggctcacgct
     3421 cgcggggggt tttctcctgg tcgccgccga ggaggctttc agccgatggc ctccattctc
     3481 tatcggggag atgccagggt actcgacccg gtcatcgcgg ttgtgggcgg tataggactc
     3541 gaacctacga cctttgcgat gtcaacgcaa cgctctaacc agctgagcta accgcccata
     3601 gaatggtgcg caaattataa ctggcagatt ggaaaaaggc aagaatggat caaggggcgc
     3661 gtgcactggc tggttcattg gcgtcaacaa acagccacaa ccagaactgc cagttcgcga
     3721 ttggcgtcga aaccaccacc ggggttggtg taggaacggg cgtgccagcc acctgggcta
     3781 aaggaaccac cgggacatcc cccgggcgca ccatactccc ctcttcataa gcccactcga
     3841 ttacagatgc gtcagaggtg gcataggcga tctgggtctg caagctatcc tgggtttgaa
     3901 tctgcccctc cagctgcgcc aataccccct cttttcgggt gctcaggcgc cgcaaggctg
     3961 acatacgatt attgaaatcc atcaccagaa tagcgagcac cacgaacccg atgatgatca
     4021 aggcgtatct ccagctaaaa ttcgggacct tcataatctc atcttaccgc agcctgccct
     4081 ttggcgcaag acccttcggt tcaagttaag acaacctccc ctgctaatca tagcaaggga
     4141 gatgttgcaa ggtgtgtgcc gaaggtggga cttgaaccca catgagcgta tgctcactac
     4201 gccctgaacg tagcgcgtct gccaattccg ccacttcggc ttgcgcgggt tattttaccc
     4261 cgattcaagg ttttgtcaac cttttatgcc tcaagtgttg ttgttcagcg atattgacac
     4321 cccctgactg ttcagggaaa atgtcacccc atggaaaaca aggtgacatt aacgatgaaa
     4381 gaacagcaca aactaaaaat ggtgattgac tatgaagccg ggaagatacg agcccagaga
     4441 gcggctgaac tgcttggcat cacgaagcgg cagtttcgcc ggttagtagc ggcctatcgc
     4501 cagcgaggg
//
LOCUS       DBNGSX010000423         3777 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_524199, whole genome shotgun sequence.
ACCESSION   DBNGSX010000423 DBNGSX010000000
VERSION     DBNGSX010000423.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_524199"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(377..889)
                     /locus_tag="AC16CB_12500"
     CDS             complement(377..889)
                     /locus_tag="AC16CB_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343335.1"
                     /translation="MDKTRFLQIISWGGAIALLLSACRMPIEIGGQPAVIGFSTDYEP
                     STSDSQTYTTNQDQSANEPTGVQEIQVDNVRVDLGIGSSVPVMVEISGTWPGPCSQLA
                     EIKQTAGEETFDIYLLATPDGPDCPLDQQGLLLEINIPLNMAGKQAGTYRVNVNGKET
                     SFDWSGSLSK"
     gene            complement(966..2558)
                     /locus_tag="AC16CB_12505"
     CDS             complement(966..2558)
                     /locus_tag="AC16CB_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343336.1"
                     /translation="MNALRALYHMARADFLERVRRYSFLIMLGMIVWLGYVVNTGVLV
                     MSVPPNYVGEINSPWVGALMTITVTFFLGWFGFYMVKGSVGRDYETGVGQIMATTPLT
                     RPLYALGKWLSNFAILSIMILILIIAGILMNLLLGSTAIDLWALVAPLLIISMPFMAI
                     VAAVAVLFETIGWLRGGLGNIVYFFMFILLLVPNIESPVYLPLLDYAGLRLLSDGIIR
                     AAKMAYPESEGGFNFTFGDSITDPHTFRYDGIAWNADVILTRLFFLLVALVIVMLAAA
                     FFDRFNPSKILIIKRKDGKLKSLEPAVTETIPLSQVHLTPSAGLPARFRFGALFIAEL
                     KLFLKGHRWWWYVITLGLVIAQLATDLKTARILLAVTWLWPILLLSGLGSREARHNTR
                     EIVFSAPRPVLNQLPATWLAAFSVIALMGSGAFLRFLFAGEITSLLAWLAGALFIPSL
                     ALTFGVLTGSSKAFEVVYVLWMYLILQEVPPLDFVGVVPESPWAVYALLALGLFTLTA
                     FVRQWQLKGGSVAKLAGKRRSI"
     gene            complement(2555..3433)
                     /locus_tag="AC16CB_12510"
     CDS             complement(2555..3433)
                     /locus_tag="AC16CB_12510"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_function: GO:0140657 - ATP-dependent activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3343337.1"
                     /translation="MKLTIKHLSKQYRRDFWGLREFDLEVGPGVIGLLGPNGAGKSTL
                     MRMLATITQPSEGTIKWNDVDIVRSPDTLRAALGYLPQDFGIYPNLSGREFSEYIAAI
                     KGLDGNAARRRIDELLVVVNLVDAAKRPLGSYSGGMKQRVGIAQALLNDPQLLIVDEP
                     TAGLDPEERVRFRNLLSDLSGERIIFLSTHIVSDVEATATEIVIIHKGRKLLHAAPEK
                     ILQSLDGQVWQWVVPSEALVALKQKHIISGTVRRQDGIQVRVVSETAPSADAQPIAPS
                     LEVVYLQLVSRNGVTR"
BASE COUNT          944 a         1004 c          973 g          856 t
ORIGIN      
        1 tatacaaata gttcaatctg cctcttggct ggcttgaccg ccatggaggt tgatttttcg
       61 tgtgagctca ttatgcttct ttccatgtgc atgcaaaatc gataaatttc ctagccagaa
      121 ataattcagt gaattaaaaa aaagcctact tgagcttttt attataacct cgtcatgtag
      181 gtgatggatt tcaaacagca taaaagaata atgagcgcgg agaggctcaa actctcaacc
      241 aatggctttt ctttacactt gcttgcttcg attgttattg aatctataat atgagcgatg
      301 gattttgata caacaagcaa gctccaagaa agctagtagc tctcacctgc tatctctagg
      361 agcctgccat gccgatctac tttgacaagc tcccgctcca atcgaagctt gtttctttgc
      421 cattcacgtt gaccctgtac gtgcccgcct gctttccagc catattaagg ggaatattga
      481 tctccaataa taacccctgt tggtcgagag ggcaatctgg tccatcgggt gtggctaaga
      541 ggtaaatatc aaaggtctct tcgccggctg tctgtttgat ttcggccagc tggctgcacg
      601 gccccggcca ggtgccgctg atttcgacca tcacagggac ggatgacccg atcccaagat
      661 caacccgtac attgtccacc tggatttcct gcacaccggt cggctcattt gctgattggt
      721 cttgattggt tgtgtaggtc tgagaatcgc tggtcgaagg ttcgtaatca gtagaaaacc
      781 cgataacagc tggctgcccc ccaatttcaa ttggcatcct acaggcgctg aggagaagcg
      841 ctatagcccc tccccagctt atgatttgta gaaatctcgt tttgtccatc gtcttttccc
      901 ttacctatat aaacatccaa tctttttagt gctggcaggt tggaatggaa gaggtaagct
      961 gcctttcaaa ttgatcttct tttgcctgcc agctttgcta cgcttccgcc tttcaactgc
     1021 cactggcgga caaatgccgt caaggtgaac aatcccagcg ccagcaaggc gtaaacagcc
     1081 cagggacttt cgggaaccac ccccacgaaa tcgagcgggg ggacctcttg aaggatgagg
     1141 tacatccaca ggacgtaaac aacttcaaat gctttgctgc tgccggtcag gactccaaaa
     1201 gtcagcgcca acgaaggaat gaacagcgcg ccagccaacc atgccagcaa gctggtgatt
     1261 tctcctgcga acaggaagcg taaaaatgcg ccagaaccca tcagcgcaat gacgctaaag
     1321 gcggccagcc acgtggcagg cagctgattc agcaccgggc gtggggcaga aaagacgatc
     1381 tcgcgggtgt tgtgccgcgc ttcgcggctt cccaacccac tgaggagcaa aatcggccat
     1441 agccatgtga cggcgagcag gatgcgggcg gttttgaggt cagtggctaa ttgggcgata
     1501 accaaaccca aggtgataac ataccaccac cagcgatgcc ccttcaggaa caatttcaac
     1561 tcggcgataa aaagcgcgcc aaaacggaag cgggcaggta agccggctga cggggtgaga
     1621 tgaacctgtg aaaggggaat cgtttcggtg acagcaggct ccagggactt caatttgccg
     1681 tccttccttt tgattattaa gatctttgag gggttgaagc gatcaaaaaa ggcggcggcg
     1741 agcatcacga taaccaacgc aactagcagg aaaaacaatc gagtcaggat cacatctgca
     1801 ttccaggcaa taccatcgta gcgaaaagta tggggatcgg tgatgctgtc tccaaaagtg
     1861 aaattaaacc cgccttcgct ttcaggatag gccatctttg cggcgcggat tatgccgtca
     1921 ctcagcagcc gtaacccggc ataatcgagc agaggcaggt aaacagggct ttcgatgttg
     1981 ggaaccagca gcaggataaa catgaaaaag tatacgatgt tgcccagacc gccacgtagc
     2041 cagccaatgg tttcaaacag gacagccaca gcggcgacaa tcgccataaa tggcatcgag
     2101 ataatcagca atggggcaac caaagcccat agatcgatgg cagtggagcc gagcagcaaa
     2161 ttcatcaaga tgccagcgat tatcaggata agaatcatga tgctgagaat agcaaagttg
     2221 ctcaaccatt tgcctagggc gtaaagcgga cgggtgagcg gagtggtcgc catgatctgc
     2281 ccgacgccag tctcataatc acggccgacc gaacctttga ccatataaaa gccaaaccag
     2341 cccaaaaaaa acgtgaccgt gatcgtcatc agcgcgccca cccagggcga gttgatctcg
     2401 cccacgtagt tgggcggcac gctcatcacc aagacccccg tgttcacaac gtagccgagc
     2461 cagacgatca tgccaagcat gattagaaag ctgtagcggc gcacgcgctc cagaaaatcg
     2521 gcgcgcgcca tgtggtagag tgcgcgcagg gcgttcatcg cgtcactcca ttccgggaaa
     2581 ccagttggag ataaacaacc tcgaggctgg gcgcgatggg ctgggcgtcg gcactgggag
     2641 cggtctcgct caccacccgc acctggatgc cgtcctggcg gcggactgtc ccgctgataa
     2701 tgtgtttctg tttaagagca accaacgctt cagaagggac gacccattgc cagacctggc
     2761 catccagcga ttgcaaaatt ttttcgggcg cggcatgtag aagtttacgc cctttgtgga
     2821 tgataacgat ttcggttgcg gtcgcttcca catccgaaac aatgtgtgta gacaggaaaa
     2881 tgatgcgctc gcccgacagg tcggagagca ggttgcggaa gcgcacgcgt tcttcgggat
     2941 cgagcccagc ggtgggctcg tctacgatga gcagctgtgg atcgttgagc agcgcctgag
     3001 caatcccaac acgctgtttc atcccgcccg agtagctccc aagcgggcgc ttggcggcat
     3061 ccacaaggtt gactacgacc agcaattcgt caatgcggcg gcgggcggca ttgccatcca
     3121 agcccttgat agcagcgatg tattctgaga actcccggcc gctcaaattt ggataaatac
     3181 caaaatcctg cggcaggtac ccgagcgcag cccgcagtgt atcgggcgac cgaacaatat
     3241 ccacgtcatt ccatttgatc gtaccttcgc ttggctgggt gatcgtcgcc agcatgcgca
     3301 tcagtgtaga cttgcctgcg ccgttcggtc cgagcaagcc gatcacgcct ggtcctactt
     3361 caaggtcgaa ctcgcgcagt ccccaaaagt cacggcgata ttgcttggaa agatgcttga
     3421 tggttagttt catttaaaac cttctttcgt caatctgtat agcgggtaga attattaccc
     3481 aagacaaatt ctctatattg cccgctttca gggctcagat atatactggt gggccgattt
     3541 tgctccgcgt gcgcgcccac atatttcagg tacacgattt taagatgcta aatgtacgct
     3601 tcctggagcc gtcggtactt agctttgttc ttcgacttta gcaactttgc ctccatctgg
     3661 aacgatctgc aattgcagga tcgttattcc cagtgcgtgc agcctgcgca ccagcccgac
     3721 aatccccgat tgatcggtct gcacattgag cacggtgata ttttccaact gcctggt
//
LOCUS       DBNGSX010000424        25798 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1009405, whole genome shotgun sequence.
ACCESSION   DBNGSX010000424 DBNGSX010000000
VERSION     DBNGSX010000424.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 25798)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 25798)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25798
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1009405"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="AC16CB_12515"
     CDS             <1..431
                     /locus_tag="AC16CB_12515"
                     /inference="COORDINATES: protein motif:HMM:NF012892.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HIR3343338.1"
                     /translation="TAFGLGLLWHLAQGDILPPGANPLTFLLGYDWRGLDVQTAETMA
                     FATLSLCELFRAYTVRSERISIFRMGVFSNRYMQYAVGFSIALLLLVVNVPFLQPIFN
                     THFLKPVEWAVVVGLALIPAISEEITKAALRWRDQRERGE"
     gene            905..1834
                     /locus_tag="AC16CB_12520"
     CDS             905..1834
                     /locus_tag="AC16CB_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HIR3343339.1"
                     /translation="MVAIYKNTEQGLSMLTEPTTGCWINAVNPTLDEIHWLQSLGIPQ
                     DYITYPLDVDERSRTERENGELLILLRIPYFQGHSADIPYTTIPLGIILNDRYLVTVC
                     KRETDILNEFADGRVKSLATSKRLRFVLRLLLSTATKYLVYLREINRTVEALEDQLQL
                     STRNKEVLELLKYQKSLTYFTTALKSNELMLERLQRSQIFKAFPEDEDLLEDVITENQ
                     QAIEMTNISSNILSGMMDAFASIISNNLNAVMKFLAAITVVLSLPTMIASFYGMNVDL
                     PLDHLPVAYIYPIAFSLIISIVVVVVFYRRDWL"
     gene            complement(2061..3998)
                     /locus_tag="AC16CB_12525"
     CDS             complement(2061..3998)
                     /locus_tag="AC16CB_12525"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3343340.1"
                     /translation="MPKEQILFFLLDTYIGQLVEDTPLRSAGYEVNLVKDFPAYKALV
                     RASLPDVVILGERLPGEQEDEEINGLDVATSLLEKHPNLPILLVANQPTDALLAEAMR
                     IGMVDCLRPPLRGDDLLLAVQRALQRGQKIKQWARGEVKRNTQSLQARLSSLETLQKI
                     GRNVTSSLDLDTILTAVVDSAVDLTGAEKGSLLLLDEATGELYMRASRNFGEDFARTF
                     RVPVLDTLAGEVLRTGKPVILDEKTPKKIKTAYLVHSLLYVPLVIEESTIGVLGVDNG
                     QSGHPFTEYHLGLVSALAAYAAVAIQNASLYARSEVERNKLEAILTRITDSVIVVGLD
                     GRLVLINQTARHAFAYKPEESLTGKPADEVIHSLDLVEILCDAEATTPFRSEIGLDDG
                     RVFNAQVTPIPEVGVAVILQDITHLKELDRIKSEFVNTVSHDLRSPLTAILGYIELIE
                     RVGPINQQQLEFIQRVEQSVQNITALINDLLDLGRIEAGFDARKEIVPLPPLVDLTID
                     SLLPRFEEKPQHLVVDIPKDLPAVLGNPIRLRQMLVNLVTNALKYTQQGGEIRVSAHA
                     AGGQVILQVSDDGVGIPRADQPYIFDKFYRASNVVREASGTGLGLAIVKSIVENHQGR
                     IWVKSSPGKGTMFTVVLPAAR"
     gene            complement(4019..5221)
                     /locus_tag="AC16CB_12530"
     CDS             complement(4019..5221)
                     /locus_tag="AC16CB_12530"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3343341.1"
                     /translation="MGNQQEKILLVESSHKNSDLIARQILEPLGYRVNVARSAPDALQ
                     EVALFSPDLIITNLKLSGLSGKDLLVALTSQGLEYPVIVLCEKGAEADLIQTFRLGAV
                     DILLWPAREVEVISAVERALNQVRARRDREALARQLQQTNQELQRRVRELTTIFAIGK
                     AVTAITDYHILFERIVEGAMYICEADSAWLLLRDEQSKAFLLRAQRNLPVSVSAKIGQ
                     PWDDGISSLVAASGEPLIIHGEPLRRFKISLLGRSAMVLPVKVNNEVVGLLAVLRKSA
                     VPFGANSQALLSAVVDYASISIVNANLFKALEERTHRLQQAANADQDEESNKDELLNR
                     LRQEMSAPLAEADQAIESLLIDEQSGLGDSQKSLLRQTQECLHRMAELLTSQPSNAKV
                     KDGLGTLN"
     gene            5456..6394
                     /locus_tag="AC16CB_12535"
     CDS             5456..6394
                     /locus_tag="AC16CB_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HIR3343342.1"
                     /translation="MEYKDYYKTLGVDRKAKEDEIKRAYRKLALKHHPDRNPNDKKAE
                     EKFKEINEAYQVLSDPQKRTRYDQLGDSYHHWQQRGGTPGGFNWDEWVTTPNQSNVRV
                     EVGNLEDLFGGGLGDFSDFFRSVFGGMSGMGTQRSSRSRAAERTRIPSSQSEVTISLR
                     EAFQGTTRRIEVDGRHLEVKIPRGARTGTKIRVAQALTSPNGSKGDLYLVIKVADEPN
                     FQSKGNDLYTDVEVNLYQAVLGSEVTVPTLSGNVVLTIPPGTQPGQTFRLIGQGMPQV
                     KNPENRGDLFVRVKVKIPRNLSAEQRELFQKLARQA"
     gene            6523..7683
                     /locus_tag="AC16CB_12540"
     CDS             6523..7683
                     /locus_tag="AC16CB_12540"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR3343343.1"
                     /translation="MGRLVFIVFILVLSTLACRAASIPEEKVVAPTSPAPRLDAITTP
                     IFIPPAADMVALQDTLVEIYQQVNPGVVALRALTSDGGSLGSGFVIDQDGHIITNYHV
                     VEAVNRLEVTFASGHKTRGEILGTDLDSDIAVIKVDVPKEELYPLTLSDSDQVQVGQT
                     VIAIGNPFGFSGTMTMGIVSGLSRTMLSLHEAPGGGMFSTGDIIQTDAAINPGNSGGP
                     LLNLNGEVIGVNRAIFSTQTNELNQPVNSGIGFAVSINMVKRVYPALIAEGKFDYPYV
                     GIASLSDLSLLQQETLGLSRSTGVYITEIVSGSPADQAGLRAGSRRTSIPGLLAGGDL
                     IIAIDQKEVINFSDFLSYLLRNKSPGDTVAMTILREDKEIEIELTLEKRPSR"
     gene            7729..8829
                     /locus_tag="AC16CB_12545"
     CDS             7729..8829
                     /locus_tag="AC16CB_12545"
                     /inference="COORDINATES: protein motif:HMM:NF037429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baeRF10 domain-containing protein"
                     /protein_id="HIR3343344.1"
                     /translation="MLTENELRELLDYQASAPVLSIYLNVEPSEGSVDAHKLRLRSML
                     RDVELPDDVQRVELFVEHEFNWSGRSVSIFSCMADDFFRSYTLAVPIRSRVRVSDKPY
                     VKPLADLLDAYGGYGVALVDKQGARLFYFHLGELREQEGVMGEAVRKTKRGGGSQAQG
                     RRGGTAGQTNYVEEVADRNIKDAADFAAHFFHENNVRRILLGGTEDNLTPFQHHLPKA
                     WRSLVVGTFPVSMTASHSEVLERAMQIGQEAERRREATLVKTAITGAAKGRGGVIHLE
                     ETLHAVHQGRVQTLLISDGFRAPGRRCKGCGYITSKKFQVCPFCGGKVEPIPDAVELV
                     VRKVMQSGGDVEVLHDAQALSEQGNIGGLLRY"
     gene            complement(8886..9500)
                     /locus_tag="AC16CB_12550"
     CDS             complement(8886..9500)
                     /locus_tag="AC16CB_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343345.1"
                     /translation="MDDEKQNEKPASAIPEATKEKVSPPPSRLNRIALRLLRWLLGIL
                     IVMGLGALLAIYTLYMPTRQKLMDSQQQVEQADQRVAELEGEVGNLSTFEEQNQDLLD
                     ELEKTTLHVTILSARADVAAAQLALAQNDPAKARLALSRTPETLKNLESMLEPGKKKL
                     AKDMQARLDLAVKEIGENAYAANSDLDVLATGLLELENAYFAKP"
     gene            complement(9526..10443)
                     /locus_tag="AC16CB_12555"
     CDS             complement(9526..10443)
                     /locus_tag="AC16CB_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343346.1"
                     /translation="MSDQTPSPPPPSFGNRLGLAFISFLRALVRVLLILLLVALLGAI
                     VYFGVPVLYQRYVQPVERDLARLNDFQARQEQDDLQFNRRLDELQERLTEIELQNDTQ
                     KQDLDELQTRLEDMESERQFGTQAGESAQATAIARLDEMETYLAELSTDLEQMQAEIT
                     ALNQALAKNSAQIQELSSRTDSGAAPLEALRIDLQLVKAMEHLTRSRLFLAQNNLGLA
                     EQDVRDARQLLTNLQGQVPAFQIDALARIIARLDAALSSLPSRPFLAAEDLEVAWQLL
                     QGGLPGEPTTQTTSTPASIQGTPTPSATP"
     gene            complement(10488..11360)
                     /locus_tag="AC16CB_12560"
     CDS             complement(10488..11360)
                     /locus_tag="AC16CB_12560"
                     /inference="COORDINATES: protein motif:HMM:NF024245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HIR3343347.1"
                     /translation="MMDDQVTQVDLNTADMDELRTLPGVGPALAERLLQARPFASIED
                     LGRVNGFGPAALERLQPHVTLSKVETSSFATDTEEELAEHEPTASVVDEISPEAEEGG
                     ASMLEPAGEEISPQMEAEMPEAPQTPKPQPASQPVTAPKAGPRPASRMEAFWLACGAL
                     VFATLLGAALSLGFLALINGGLSYARPSQINQLARQIDGTKAQTGILEQDMDTLRARI
                     DNLEGLSGRVGTVEKDMEGLREDMDAALSSMDALSLQVENLDGQVAEIEARTERFQGF
                     LEGLQELLGGLFSP"
     gene            complement(11379..12107)
                     /locus_tag="AC16CB_12565"
     CDS             complement(11379..12107)
                     /locus_tag="AC16CB_12565"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR3343348.1"
                     /translation="MIPLRDTIRSRSFPLVNLGIIGLNTLVFLFELNLSPRGLNQFIF
                     TFGMVPARLNLTTPLALLDNPLPLITLFSHMFMHGGWVHFLSNMWTLFIFGDNVEDRM
                     GGGRYFVFYILAGLASGLLQALVAPGSSVPAIGASGAIAGVLGAYFILYPGGRVLTLI
                     PIFIFPWFVEIPAIIYLGFWFVSQLFSGLAALNMPAEASMGGVAWWAHIGGFIFGILA
                     YRFFVRRIQPIYYRQFPRFPEDSF"
     gene            complement(12240..12668)
                     /locus_tag="AC16CB_12570"
     CDS             complement(12240..12668)
                     /locus_tag="AC16CB_12570"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3343349.1"
                     /translation="MTFYISPYRRMATLRNMMNRMFEESLDETPNGEREMMLAVDVKA
                     EDEAYDIVALVPGLEPEDINIEIINNTVTLRGEFKSVSDEDGKSLVNELPAGRFRRVI
                     TLPVALDSSKTEASIKNGVLHLRVPKAEAHRPKSIKVVAA"
     gene            complement(12911..13645)
                     /locus_tag="AC16CB_12575"
     CDS             complement(12911..13645)
                     /locus_tag="AC16CB_12575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3343350.1"
                     /translation="MLARLYHAHHNAHQEDLPFWLDLARLQGDPILELGCGSGRVLLP
                     LAQAGHFVLGLESDPDMLVVLQENVPPALAERVGTIQADMAAFELGQQFALILLPCNT
                     FSTLTAEARRATLDCVQRHLAPQGLFAVSLPNPTLLRKLLGRSQPEVEESFPHPLDGE
                     PVQVSSSWVRTTGHFVIRWDYDHLLPDGNVERFSMQARHALLSTDDYIAELEAAGLSQ
                     AALYGDFDRSPYSHQAPYLIVTARRK"
     gene            13692..13765
                     /locus_tag="AC16CB_12580"
     tRNA            13692..13765
                     /locus_tag="AC16CB_12580"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13726..13728,aa:Pro,seq:tgg)
     gene            13803..14114
                     /locus_tag="AC16CB_12585"
     CDS             13803..14114
                     /locus_tag="AC16CB_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343351.1"
                     /translation="MARIKFDGVVEAVRYKPSGEIDWVRAYERRGPTFSDYILLDRET
                     LIKRIKAGKRYVSGKRIPYMASTFETSSPIRLVTNSGKEIVVTSDPKATQDRLEGVPT
                     I"
     gene            complement(14215..14958)
                     /locus_tag="AC16CB_12590"
     CDS             complement(14215..14958)
                     /locus_tag="AC16CB_12590"
                     /inference="COORDINATES: protein motif:HMM:NF016300.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HIR3343352.1"
                     /translation="MRKKVFLGIALLLVAALLSAFSSGTEVLAVTQQGPGPETPPRQL
                     NVTGSGKAFVTPDIAYINIGVHTENADAAEAVASNNARSKKVADAVKKFNIDPKDIQT
                     TNFSIFPQQQYDPTGKITGVVYFVDNTVFVTLRDISKVGDVIGAAVGAGANSINSIQF
                     DAADRSKALSEARKSAVADALATAKELASAAGVTLGDIQMINSYGGGAPVPIYDAKGY
                     GGGGAGAPVPVSPGQLVLMVDVNIVFEIK"
     gene            15219..16757
                     /locus_tag="AC16CB_12595"
     CDS             15219..16757
                     /locus_tag="AC16CB_12595"
                     /inference="COORDINATES: protein motif:HMM:NF016013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HIR3343353.1"
                     /translation="MSENLTPPPPSPRLLMLYRIRRMMPRIDRGQRAEVQVLLRNASH
                     PNFDFFILVVLSSIIATLGLLTDSPAVIIGAMLVAPLMSPIIGLGLASLTADGRLLGS
                     AASALIRGALLAIVISFLISAINRLLPLIALQAQDLPGEVLARTHPSPIDLGIALAGG
                     MAAAFALAMPNISAALPGVAIATALMPPLCTVGIGLAWGRQDITGGALLLFVTNAVTI
                     AFAAILVFSALGFSPRRKEGAGLFPRSLQIAALLTLILMIPLTLFSVRFVQEATSDRT
                     IAQVIDENVRAFGDAELVEWDSNQEAGTLKINITMRTNKPLRLEDSIAFQKAVGGNLH
                     AQGVLGDNEKVQVVINQVLAERLDPLIPPTSTHTPTVTFTPTPGPSATPSLTPTPTAT
                     YTPSPTSSPTATITPTATPVPTDTPTPALALGASASLPSFRLRQSPEGPAIGPALRTG
                     SPLTVLYGVQIVDGLVWVEVQDSEGRVGWVPQIYLKLITPTPTRTPSLTPTALTPTSS
                     PTPP"
     gene            16966..19452
                     /locus_tag="AC16CB_12600"
     CDS             16966..19452
                     /locus_tag="AC16CB_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR3343354.1"
                     /translation="MRFDRFTERAQEAAQRAAEIIQRYGHNQIDTEHILLALIEQPQG
                     VIPQILEFLKVDAAALIERLDYILRTSPKANIFGGGAGQIFITPRVKRIIDLANEEAN
                     RLKDEYISTEHIFLAILSERSTPAARLLEGAGVTRERVYDTVQQIRGGQRVTDPQAET
                     RYRTLEKYSRDLTQSAREGKLDPVIGRDAEILRVIQILSRRTKNNPVLIGEAGVGKTA
                     IVEGLAQKIASNDVPEILTGKRVVALDLGAMIAGSRFRGEFEERLKVAIEEVQRAAGE
                     VILFIDELHTVVGAGAAQGAMDASNMLKPALARGELQCVGATTLDEYHKHIEKDAALE
                     RRFAPVFVEEPNVEDTIQMLRGLRDRYEAHHKVRFSDDALVAAARLSARYVTDRHLPD
                     KAIDLMDESAAKLRVALYSLPPEIKDLKVEIDRLAAEEEQSGLERDYERAAQKKAERL
                     RLETEFNEKRDQWEAEHQLDEVVDVNDIAEVVAQWTGIPLHQMMETEAEKLLQMEVRL
                     HERIKGQDEAIKAISDAIRRARSGLKDPRRPIGSFIFIGPSGVGKTELARALARFMFE
                     DEDALVRIDMSEYREQHTVSRLFGAPPGYVGYEEGGQLTEAVRRRPYRVILFDEIEKA
                     HPEVWNALLQILDDGRLTDGQGHIVDFRNTVLIMTSNLGTEFVRKAGSLGFLQRKDDD
                     EERAAHEKIEKALKSTFRPEFLNRIDEIITFSPLSLEQMREIVDLQMNEVQERLSEHG
                     LVVELSEEARVWLANVGYDPSFGARPLRRALQKHVESPLSVSLLSGEFSSGDKILVEV
                     DEEKNELTFCRVEQASDQSIPAEEVSQVEA"
     gene            19714..20220
                     /locus_tag="AC16CB_12605"
     CDS             19714..20220
                     /locus_tag="AC16CB_12605"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3343355.1"
                     /translation="MSSDIFDQLPLFDGFTSDQRELVRPLFTPVDCYSGTVLFEQGDP
                     ADYLYLVVVGEVVIRYKPEDGPEILVARVRPWGIVGWSAALGSHAYTSGAICEEYTQM
                     LRLYGKDLRNLCEQNPETGILILERLATVIAQRLRNTHEHVMELLRQGLGTNSNYAKE
                     GSHGRSRL"
     gene            20201..20437
                     /locus_tag="AC16CB_12610"
     CDS             20201..20437
                     /locus_tag="AC16CB_12610"
                     /inference="COORDINATES: protein motif:HMM:NF013286.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HIR3343356.1"
                     /translation="MVDPVYEDRLRALIERLSAYIEHYHGGSVQYISLDGSVLKVRLG
                     GACLGCPLLPSTLHGWVEGTVRQFFPEIERIEEV"
     gene            complement(20492..21019)
                     /locus_tag="AC16CB_12615"
     CDS             complement(20492..21019)
                     /locus_tag="AC16CB_12615"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HIR3343357.1"
                     /translation="MLISKELEEAINTQVGREFGASLQYNSIAAYFDADNLPSLAAFF
                     YRQASEEHMHAMKFLNYVVDAGGQVRIPAISASKYDFSSAEEAVQLALDWEQEVTRQI
                     NELMNLAIQQKDHIAQDFLRWFVTEQLEEISTMDRLLSIVRRSKDNVLFVDDYISRNP
                     IVSPEEAGGEAESGA"
     gene            complement(21158..22846)
                     /locus_tag="AC16CB_12620"
     CDS             complement(21158..22846)
                     /locus_tag="AC16CB_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010897040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIR3343358.1"
                     /translation="MPIHRMFGNGGYEFNSRRSPVLGRGGMVATSQPLAVAAGLEILS
                     KGGNAADAAVAVAAALNVTEPTSTGIGGDCFALYYDAATCQVSAMNGSGRAPAALTLE
                     RLQREGFGTELPPYHPYTITVPGACAGWCDLVERHGRLLLKDVLTPAIRLADEGFPVT
                     PVTAYFWDRGVTRQLGHAPGGLELTIEGRAPRPGELFRNPGLARTFRAVAQAGKQAFY
                     QGAIAEAIAATVQAAGGCLTTDDLAAHESTWEQPIHTTYRGLRVWECPPNGQGLAALI
                     ALNILEGYDLASMEPLSATRLHLQIEAMRLAFADTRWYVADPAFNPAPVDELLSKDYA
                     ASRRRLIDPARANLEFQTGTPTAASDTVYLSVVDGEGNACSFINSNYMGFGTGIVPTG
                     WGFSLQNRGHNFGLNAGHPNALAPGKRPYHTIIPGMITVDREQSSTVDRRPAQANHQL
                     VASFGVMGGFMQPQGHMQVLVALADDKLDPQAALDQPRFCIQDGEAGGGVALEEGISL
                     QVKSELALRGHPVEMVAGYDRALFGRGQIILRDAESGVLWGGSDPRADGCAMSV"
     gene            complement(22979..24043)
                     /gene="selD"
                     /locus_tag="AC16CB_12625"
     CDS             complement(22979..24043)
                     /gene="selD"
                     /locus_tag="AC16CB_12625"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(23978..23980),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HIR3343359.1"
                     /translation="MSHFNPSGRQIRLTSLSSCAGUASKLSAEALAQVLRPIKDIFNP
                     ADYPDLLVGLSDPDDAAVWRLDERRALVVTTDFFTPVVDDAYDYGAIAAANALSDIYA
                     MGGQPFLALNVAALPPDLPAELLGEILRGGAEVARQAGVVIAGGHTVQDKEPKYGLIV
                     VGFVDPERLITKSGARQGDVLVLSKPLGFGTTTTALKRGQADPTDVSEVVGWMKRLNK
                     AASELAIEAGVRGGTDVTGFSLLGHGWEMAEASGVGLRFFFGKIPFTRGARRYAHEGI
                     FPGGSADNRLYYGDHVRFDEGIDEPSQMLLFDAQTSGGLLLCVPRQKLEGLLSRAAQV
                     GQVLWEVGEVVDGEIIEVLR"
     gene            complement(24116..25183)
                     /locus_tag="AC16CB_12630"
     CDS             complement(24116..25183)
                     /locus_tag="AC16CB_12630"
                     /inference="COORDINATES: protein motif:HMM:NF013324.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HIR3343360.1"
                     /translation="MNVASTIKIIASLSWFVFLGIIALAVGRASRRQPLRGATTIIIV
                     VGVLALLLSTASAGLVFVSPQERGVVITALDPGGYRPQALQAGLRWVVPFVENVVTYP
                     ISRQTYTMSIAPLEGQVQGDDSVAARTADGQEIFVDASVLYAVDPEEVVQLHIRWQNR
                     YIDDLVRPLSRGIIRDAVSQFRVEEVVTSKRFELVDQIRTNMSAKLDENGMILVDFVL
                     RNITFSPEYAASVEQKQIAEQQAQQARLVVEQRKQEAEQARQSAQGQADAAVIRAKGD
                     ADARLIQADAEAKALLLIADALQDNPELITYQYINRLAPGVQVMLVPSDNPFLLPLPT
                     LEPGVVEPSPSPSPSPPATTP"
     gene            25436..>25798
                     /locus_tag="AC16CB_12635"
     CDS             25436..>25798
                     /locus_tag="AC16CB_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343361.1"
                     /translation="MCYHAPVRAQTARVIEIQQDPGGFSAWLTCPAQVIPAAGQYALA
                     IDSKDALQPLSMPLFPMAYSKDSFLAASPYQIAWTPGTALEIHGPLGHGFKLPPTARN
                     VALVGLGETSARLMPLIPT"
BASE COUNT         5960 a         7288 c         6955 g         5595 t
ORIGIN      
        1 taacagcttt tggcctgggc ttgttgtggc acctggccca gggggatatc ctgccaccag
       61 gcgccaaccc gctgaccttt ctgctgggtt acgattggcg cggattggac gtgcaaaccg
      121 cggagacgat ggctttcgcc accctctcgc tttgcgagct gtttcgcgcc tacacggtgc
      181 gttccgagcg gatctcgata ttccgcatgg gcgtgttctc caaccgctac atgcaatacg
      241 cggtggggtt ctccatcgcc ctgctgctgc tggtggtgaa cgtgcctttc ttgcagccga
      301 tcttcaacac ccatttcctc aaaccagtcg agtgggcggt ggtagtcggt ctggcgctga
      361 tcccggcgat ctccgaggag atcactaaag ccgctctgcg ttggagagat caaagggaga
      421 gaggggaatg aatcgcttgc cccccaggat cacctgtgaa agaggaatca aaagggtact
      481 ttttcctgac aatagcatga gcgtcacttg cgccaatatg gcgaatggga tatactaacg
      541 gcaaggcatg ttatcagatt ttgtttgcgg tttggaactt attgcaatgg gatgccccct
      601 tatgcgggga cactccactg cacccaaacg taccaattta tataaggagg ctccggctta
      661 gctaggagtt gcgcgcgcac gtccacgctt ctcgaaacgc cggcctcgtc tggtctgcga
      721 caaaatttcg agaagcgttt tgttttatta tttggagaag aaagataagg agtaagacaa
      781 tgaaagcgaa gagaggggca cgtccctgcc atcgctcacc atcacaagat atcacccggg
      841 gcgagtccga taagccgccc tgactttaac cctgcgggca gttgcccgga aaggagagaa
      901 acgcatggtc gctatctaca agaacactga acaaggtctg tccatgctga ccgagccaac
      961 caccggttgc tggatcaacg cggtgaaccc caccctcgat gaaatacatt ggctgcaaag
     1021 ccttggaatc ccacaagatt atattaccta ccctttggat gtcgatgagc gatcgcgcac
     1081 ggagcgggaa aacggcgagc tgctcatctt actgcgcatc ccttacttcc aggggcatag
     1141 cgccgatata ccctatacta ctatccccct gggcattatt ctgaacgacc gttacctagt
     1201 cacggtctgc aaacgcgaaa ccgatatcct gaacgagttt gctgatggcc gcgtgaaaag
     1261 cctggcgacc agcaagcgcc tgcgctttgt gctgcgcctg ctgctcagca ctgccaccaa
     1321 atacctggtt tacctgcgcg agattaaccg aacagtagaa gctctggaag accagcttca
     1381 gctctcaacg cgcaacaaag aggttctgga gctgctcaag tatcaaaaaa gcctgactta
     1441 tttcaccaca gcgctcaaat cgaacgagct gatgttggaa cgattacagc gcagccaaat
     1501 cttcaaggct tttcctgagg acgaggacct gttggaagac gtgatcaccg agaaccaaca
     1561 ggcgatcgaa atgaccaaca tctccagcaa cattctcagc ggcatgatgg atgcttttgc
     1621 ttcgatcatc tcgaacaacc tgaatgcggt catgaagttc ctcgctgcca tcacggtggt
     1681 gctcagcctg cccaccatga tcgccagttt ctacgggatg aacgtagatt tgccgctgga
     1741 ccatctacct gtggcgtata tctaccctat tgcgttctct ttgatcatct cgatcgtcgt
     1801 agtggtcgtc ttctaccggc gcgattggct gtgaggtgcg cttgtgagta atatcacata
     1861 caactgatga tctttgacaa taactttgga gatgtaaatt tagtatgata agtctagaaa
     1921 acgggatttc aggataatct cctgatgttc atcgctgttt cctcctttgg cgaaagcctc
     1981 cgccgggttc tcctcaccgc cccggcggag gcgattttaa cgacacatgg gctcacgcag
     2041 ttgaaggaaa agcggggggc ttagcgcgcc gccggcagga cgacggtgaa catggtaccc
     2101 ttacctggtg acgactttac ccagatacga ccctggtggt tctcaacaat cgatttcacg
     2161 atcgccagcc ccaggccagt cccagatgct tccctgacga cattgctcgc ccggtagaat
     2221 ttatcaaaga tatatggctg atcagcccgg gggataccta ccccatcatc actaacctgt
     2281 aaaatcacct gcccccccgc cgcatgagct gaaacacgaa tctcacctcc ctgctgggtg
     2341 tatttgagcg cattggtgac caggtttacc agcatctgcc gaagccgaat ggggttcccc
     2401 agcaccgcag gaagatcctt aggtatatct acgacaaggt gttgcggttt ttcctcaaag
     2461 cgcggcagca agctatcaat tgtgagatcc accaatggcg ggagaggcac gatctctttg
     2521 cgggcatcga acccagcttc gatccgaccc aggtcaagta aatcgttgat cagcgcagtg
     2581 atgttctgca cgctttgctc gacacgctgg atgaactcaa gctgttgctg attaatcggt
     2641 cctacccttt caattagctc gatgtaaccc aggatggcag tcagggggga gcgcaagtcg
     2701 tgggagacgg tgttgacgaa ctcactcttg atgcgatcaa gctccttcaa gtgggtgatg
     2761 tcttgcaaga tgaccgccac tcccacttcg gggataggag tcacctgagc gttgaagacg
     2821 cgcccatcgt ccagtccgat ttcgcttcga aagggtgttg tggcttctgc gtcgcagaga
     2881 atctcgacca ggtcgagaga gtgaataact tcgtctgccg gcttgcccgt cagactttct
     2941 tcaggtttat aagcaaatgc atggcgggct gtctggttga tgagcaccaa gcgcccatcc
     3001 aagccaacca cgatcacgct atcggtaatg cgcgtcaata tggcttcaag tttattacgc
     3061 tcgacctcgg agcgcgcata cagtgaagcg ttctgaatag caacggcagc gtaagctgcc
     3121 agcgctgata ccagaccgag atgatattca gtaaaaggat gcccgctttg tccgttgtcc
     3181 acaccaagca caccgatggt gctctcctct attactaagg gcacatacaa taagctgtgc
     3241 accaggtaag ctgtctttat tttcttaggg gttttctcgt ccagaatcac aggtttgcca
     3301 gtgcgcaaga cctcacctgc aagcgtatcc aatactggga cacgaaaggt gcgcgcaaaa
     3361 tcttcaccga agttgcgcga ggcgcgcata tacagttcgc cagtcgcctc atcgagcagc
     3421 aacaggctgc ccttctctgc gccagtaaga tccaccgcag aatccaccac agcagtcagg
     3481 atggtgtcca aatcgaggga tgaggtcacg ttgcgcccga ttttttgcaa ggtctccagg
     3541 ctgctcagac gcgcttgcag agactgggta ttccgcttga cctcgccgcg cgcccattgc
     3601 ttgatctttt gaccacgctg caaggcgcgc tgcactgcaa ggagtaagtc atcgcctcgc
     3661 aagggagggc ggagacaatc aaccattcca atccgcatgg cctcagccag gagggcatcc
     3721 gtgggctgat tggcaaccag caaaatgggc aaattgggat gcttctcaag caggctggtg
     3781 gctacatcca acccgttaat ctcctcatct tcctgctcac caggtagtct ttctcccaag
     3841 atcacaacgt ctggcaggct tgctctcacc aatgccttgt aggctggaaa atctttcacc
     3901 agattgactt catacccagc cgatcgcagg ggggtatcct cgaccaactg gcctatatat
     3961 gtatctaaaa gaaaaaaaag gatctgttct ttaggcattg cgttcgcaac ttcctgcttc
     4021 agtttagtgt ccctagccca tctttgactt tcgcattgga gggttgggat gtcaatagtt
     4081 cagccatacg gtggaggcac tcctgcgttt ggcgaaggag gcttttttgt gagtccccca
     4141 gtccactctg ctcgtcgatg agcagtgatt cgatggcttg atcagcttcg gccagaggcg
     4201 cgctcatttc ctggcgcagc ctgttaagca gttcatcttt gttactttct tcatcctgat
     4261 cagcattggc ggcttgctgg aggcggtggg tgcgctcctc cagcgctttg aataggttag
     4321 cattgacaat agatatggat gcgtagtcta ccacggcact caataaagcc tggctattgg
     4381 cgccaaacgg caccgccgat ttgcgcaaga cggctagcaa tcccaccact tcgttattca
     4441 ccttgaccgg cagcaccatt gccgacctcc ccaatagcga gatcttgaaa cgccttaacg
     4501 gttcgccgtg aatgatcagt ggctcgccag aggcagccac cagcgagcta atcccatcgt
     4561 cccagggctg gcctatctta gcagagacgg atactggcag gttacgctgg gcgcgtagaa
     4621 ggaacgcttt gctctgctca tcgcgcagca gcagccaagc actgtcagcc tcgcagatat
     4681 acatggcgcc ttcgacgatg cgctcaaaca ggatatggta atctgtgatg gctgtaaccg
     4741 ccttgccaat ggcgaagatg gtggtcagct cacgcacccg gcgctgaagt tcctggttgg
     4801 tctgttgcaa ttgccgagcc agggcttccc gatcgcggcg cgcacgtact tgattgagag
     4861 cacgctcaac cgccgagatg acctcaactt cgcgcgccgg ccagagcaag atatccaccg
     4921 caccaaggcg aaaagtttgt atgagatcag cctccgcccc cttttcgcac aggacaatca
     4981 caggatattc gagaccctga gaagtaaggg ctaccaacaa gtccttgccg ctcagaccgg
     5041 ataatttcag gttggtgatg atcaggtcgg gggagaaaag cgcaacttcc tgaagagcat
     5101 caggtgctga tctggcgaca tttacgcgat aaccgagggg ttccagtatc tgccttgcga
     5161 tcagatcact atttttgtgg ctgctctcaa caagcagaat cttttcctgc tggttgccca
     5221 tgagataatc atagcatggt tgagtgaaag atggggacag gaaaattgac aattggctac
     5281 ttggagggca aagaaacgtt agaataatat aaatgttgcc aaacatactt gactttgtac
     5341 gcatgacgcg catacaatca tagtagtgat gtagaagtta tgcagtcgat gagcatttca
     5401 ttacactgca taagtcctac aattattgaa gtggtttgac catcaagttg gctcaatgga
     5461 atataaagat tattacaaga ctttgggtgt agaccgtaag gcaaaagaag acgagatcaa
     5521 gcgtgcatat cgcaaattag cgctgaagca tcaccccgac cgcaacccaa atgataaaaa
     5581 ggcagaagaa aaatttaaag agatcaacga agcataccag gtcttaagcg atccgcaaaa
     5641 gcgaactcgc tacgaccaat tgggtgattc ctaccatcac tggcaacaga gagggggaac
     5701 gccggggggc ttcaactggg acgagtgggt taccacacct aaccaaagca atgtgcgcgt
     5761 ggaggttggc aacctggaag atctttttgg tgggggtctg ggtgactttt ctgacttctt
     5821 ccgtagtgta ttcggcggta tgtctggtat gggaactcag cggtcaagtc gcagccgggc
     5881 ggcagagcgc accaggatcc catcctctca gtccgaggtg accatcagcc tgcgcgaagc
     5941 attccagggg accacgcgcc gcatcgaggt agatgggcgc catctagagg tgaaaattcc
     6001 gcgcggggcg cgcaccggca caaaaatccg cgtcgctcag gcactcacct cacccaacgg
     6061 gagcaagggc gacctgtacc ttgtgatcaa agtagctgat gagcccaact tccaaagcaa
     6121 gggaaacgat ctctacacgg atgtggaggt aaatttatat caagctgtac tggggagcga
     6181 ggtcaccgtg ccaacattga gcggcaacgt ggtattaacc atcccccctg gcacacaacc
     6241 aggacagact ttccggttaa tcgggcaagg catgccccag gtcaagaatc ccgaaaaccg
     6301 cggcgactta ttcgtgcgag taaaagtcaa gatcccacgc aatctctcag cagagcagcg
     6361 cgagctgttc caaaagttgg ctcgccaggc ctagaacact ggcggcaatg ggaagacgct
     6421 cttcgcaagg tgggtttaaa tacccgccga ggaatttaag aatgggcgta tcaacagatt
     6481 gcacgtaagg gaggctaatt taggagtagt ttgatgcatc gaatgggtag actagttttt
     6541 attgtattca tcttggttct ctcaacgctg gcttgccggg cggcgagcat cccggaagag
     6601 aaagttgtcg cccccacatc ccctgcgccc cgactcgatg ctatcactac acccatcttc
     6661 atcccacctg ctgccgatat ggtcgcatta caagacacgc tggttgagat ctatcagcag
     6721 gttaacccgg gtgtcgtcgc gctgcgcgca ctcaccagcg acggcggcag cttgggctcg
     6781 ggctttgtga tcgatcaaga tgggcacatc atcaccaact atcacgtggt tgaagcagtg
     6841 aacaggctgg aggtgacttt tgcctctggg cataaaacgc gcggcgaaat cctgggtaca
     6901 gacctagact cagatatcgc ggtcataaaa gtcgacgtgc ccaaggaaga actctatccg
     6961 cttaccttga gcgattccga ccaggtgcaa gtagggcaaa cggttattgc tatcggtaat
     7021 ccatttggtt tcagcggtac catgaccatg gggatagttt ctggattaag ccgcaccatg
     7081 ttatccctgc atgaagcccc cggtggtggc atgttctcca ccggagatat catccagacc
     7141 gacgccgcca tcaatccggg aaattctggt gggccgctac tcaacttaaa tggggaggtg
     7201 atcggcgtaa accgggcgat cttcagcacc cagaccaacg agctgaacca gccggtaaac
     7261 tctggtattg gatttgcggt ttccataaat atggtcaaac gcgtctaccc agccctgatc
     7321 gccgaaggca agtttgatta tccttacgtg ggtattgcca gcttgagcga tctatctctg
     7381 ctgcagcagg aaaccctagg gttatctcgc tccacagggg tgtatattac cgagatcgtc
     7441 tccggcagcc ccgccgacca agccgggctg cgagcgggca gtcggcgtac aagcatccca
     7501 gggctgctgg ccggtggaga tctgattata gccatcgatc agaaagaggt aataaacttt
     7561 tcggattttc tgagctacct tttaagaaat aaaagccccg gcgacacagt cgccatgacc
     7621 atcctacgcg aagataagga gatcgaaata gaacttaccc tagaaaaacg gccctcacgt
     7681 tagattccat cgctaacaat tcgcccaaac catatagagg aggttcatat gctaactgaa
     7741 aatgagctac gcgaactgct tgactatcag gccagtgcgc cagttcttag catctatcta
     7801 aatgtggagc catctgaagg cagtgtagac gcccataagc tacgtcttcg tagcatgcta
     7861 agagatgtcg aattgccaga cgatgtccag agagtggagc tttttgttga acatgagttt
     7921 aactggtcgg ggcggagcgt gtcgatattt tcctgtatgg ccgatgattt ctttcgcagt
     7981 tacaccctgg cagtgccgat acgcagccgg gtacgtgtca gtgacaaacc gtatgttaaa
     8041 ccgttggctg atctactaga tgcgtatgga ggttatggcg ttgctttggt tgacaaacaa
     8101 ggggcgcgcc tgttttattt tcacctggga gaactgcgcg aacaagaagg ggttatgggg
     8161 gaagctgtgc gcaagacaaa gcgcgggggc ggctcccagg cccaagggcg gcggggggga
     8221 acagcaggac agaccaacta tgtagaggaa gtggcagaca ggaatatcaa ggatgctgcc
     8281 gatttcgcgg cgcacttttt tcacgagaac aatgtgcgcc gcatcctttt gggtggcacc
     8341 gaggataacc tgacgccctt ccagcaccac ctcccgaaag cctggcgaag cctggttgtg
     8401 gggactttcc ccgtgagtat gaccgccagc cacagcgagg tactggaacg cgccatgcag
     8461 attggacagg aggcagagcg gcggcgcgaa gccacgttgg tgaaaacagc cattaccggc
     8521 gctgcaaagg gtcgtggcgg tgtgatccac ctggaagaga cgcttcacgc agtccaccag
     8581 gggcgagtgc agaccttgct catcagcgat ggcttccgcg ctccagggcg gcgctgcaag
     8641 ggttgcggct acatcacgtc aaagaagttc caggtctgcc cgttctgtgg cggaaaagtt
     8701 gagccaattc ccgacgcagt ggaattggta gttcgcaagg tgatgcaatc cggcggagac
     8761 gtagaggtgc tccatgacgc acaggccctg agcgaacagg ggaatattgg aggtttactg
     8821 cgctattgat ccaaagattg cccaaaaagg ttgaagcggg ccataagcag cccgcttcaa
     8881 tctttttaag gctttgcgaa gtatgcgttt tccagctcta atagcccagt cgccagtaca
     8941 tccaggtcag agttggctgc gtaggcattc tccccaatct ccttgacagc caaatccagg
     9001 cgagcttgca tatctttagc cagcttcttt ttaccgggtt ccagcatgct ctctagattt
     9061 tttagcgtct ctggcgtcct gctcaaggcc aggcgcgctt tggccgggtc attctgcgcc
     9121 aaggccaatt gtgctgcggc cacatccgcg cgagcgctca ggatagttac atgcaaggtg
     9181 gttttttcca gctcatctag caggtcctgg ttctgctctt caaacgtcga aaggttcccc
     9241 acctcgcctt ccaactcagc gacacgctgg tcagcttgct cgacttgctg ctggctatcc
     9301 atcagcttct gccgggtggg catgtagaga gtgtaaatag ccagcagggc acccagcccc
     9361 atgacgatca atatgcccag cagccatcgc aataggcgca atgcgatgcg gttcaagcgg
     9421 ctaggtggcg gactgacctt ttccttcgtc gcttcaggta tggctgaagc cggtttttca
     9481 ttttgcttct cgtcgtccac ggtgttcttc tccaaaggtt gtatattagg gcgtagcgga
     9541 aggtgttgga gtgccttgaa tggatgccgg cgtggaggta gtctgcgtag taggctcccc
     9601 aggtaaacca ccttggagca actgccaggc gacctccagg tcttcggcgg ccaagaaagg
     9661 cctggagggc aggctgctca gtgcggcgtc caagcgggct atgattcttg ccagggcgtc
     9721 gatctggaaa gcaggtacct ggccctgcag gttggtcaac aactgccgcg catctcgcac
     9781 gtcctgctca gccaaaccca ggttattctg tgccaggaac aagcggctgc gcgtcaggtg
     9841 ttccatcgcc ttaaccaatt gtaaatcgat acgcagtgcc tccaaggggg cggcgcccga
     9901 atcggtccgg ctgctcaact cctggatttg agcactgttc tttgctaaag cctggttcaa
     9961 ggcggtaatc tccgcctgca tttgctccag gtcagtgctc aactcagcca ggtatgtctc
    10021 catctcatcc agacgagcta tagcagtcgc ctgggcgctc tctccggcct gggtgccaaa
    10081 ttgcctttcg ctctccatat cttcaagccg ggtttgcaat tcatccagat cctgcttttg
    10141 ggtatcgttc tgcagctcga tttccgtcag gcgttcctga agctcatcta gccggcggtt
    10201 gaattgaaga tcgtcctgtt cctggcgggc ttggaaatcg ttcaggcggg ccagatctct
    10261 ctctactggc tgcacatagc gctggtaaag gactggaaca ccgaaataaa ctatcgctcc
    10321 cagcaacgca accagcaaga gaatcagcag aacgcgtacc aaagcacgca agaaactaat
    10381 gaaggccagc cccagacggt ttccaaacga aggtggtggg ggcgagggag tttgatctga
    10441 catgagacct ccttcttgaa taagacgtta tagggtatcc aaagcgctca gggtgagaac
    10501 aagccaccca aaagctcttg caacccttcc aagaatccct gaaagcgctc tgtgcgcgcc
    10561 tctatctcgg ccacctggcc atccaggttc tccacctgaa ggctcagcgc atccatactg
    10621 gacagcgcgg cgtccatatc ttcacgcaaa ccttccatat ctttctcaac cgtaccgacg
    10681 cgcccactca agccctcaag gttatcaatg cgggcgcgca aggtgtccat atcctgctcc
    10741 aaaattcccg tctgggcttt ggtaccatcg atttgcctgg cgagctggtt gatctgggaa
    10801 ggtctggcgt aactcaaccc gccgttgatc agggccaaaa agcccaggct cagtgcagcg
    10861 cctagcaggg tcgcaaagac caaagcgccg caagccaacc aaaaagcctc catgcggctg
    10921 gcaggacgcg gtcctgcctt gggagcggtg accggttggg aagccggctg cggcttagga
    10981 gtttgtggcg cttctggcat ctctgcctcc atttgggggg atatttcctc gccagctggt
    11041 tccaacatac tcgcgccgcc ttcttccgct tccggtgaaa tctcatccac caccgaggcg
    11101 gttggctcgt gctctgccag ctcctcttct gtatctgtgg caaaactgga agtttccacc
    11161 ttagaaaggg tcacatgagg ctgcaagcgt tcaagtgccg ctgggccaaa gccgttcact
    11221 cgccccaggt cctcaattga agcgaacggg cgtgcctgca agaggcgctc cgctagggcc
    11281 ggaccaacac ccggcaaggt tctcaattca tccatatctg cagtgttcag gtctacctga
    11341 gttacttgat catccatcat tgacctcctg ctcaatgtct agaaggaatc ttcaggaaag
    11401 cgcgggaact ggcgataata gatcggctgg atacgcctta caaaaaaacg ataggccaga
    11461 atcccgaata taaatccgcc aatatgggcc caccaggcaa cccctcccat gctggcttcg
    11521 gcaggcatgt tcaatgcagc caagcccgaa aaaagctggg atacaaacca gaaccccaga
    11581 tagataatgg ctgggatctc gacgaaccag ggaaaaataa agatggggat cagagtcaag
    11641 acccgcccgc ccgggtaaag gatgaaatac gcacccaaga cgccggcgat tgctccactt
    11701 gcgccaatgg cagggacgct gctaccgggc gcaaccagcg cctgcaacag cccggaggct
    11761 aaaccggcga ggatataaaa cacgaaataa cgtcctccgc ccatgcggtc ttccacgtta
    11821 tcgccgaaaa taaacaaggt ccacatattg ctgagaaaat gcacccaccc accgtgcata
    11881 aacatgtgtg agaacagggt aataagaggc agcgggttat ccagcaacgc cagaggggta
    11941 gtgagattca agcgggctgg aaccatacca aatgtaaata tgaattggtt cagccctcga
    12001 ggcgagaggt tcagctcaaa aagaaagacc agggtattca ggccgatgat ccccaaatta
    12061 accaggggaa acgaccggga gcggatggta tctcttaaag ggatcatgta aactgttcgc
    12121 tgggggcact acccccaggg ctaactaaag tataaccgga aaagggtatt tctgttaaat
    12181 aacgacgttt cggcgagaac attctcgccg aaacgtcaca ttagggtaaa agataatttt
    12241 taggctgcga cgactttaat gctcttgggg cgatgggcct cagccttggg gacgcgcaga
    12301 tgcaaaacac catttttaat gctagcttct gtcttgctag aatcgagagc aaccggcaag
    12361 gtgataactc ggcggaagcg accagcgggc agttcgttca caagggattt accatcttca
    12421 tcggatacgc tcttgaactc gccgcgcaag gttaccgtgt tattaataat ctcgatattg
    12481 atgtcctcgg gttcaagtcc cggaaccagt gcaacgatat cataggcttc atcttctgcc
    12541 ttgacgtcca ctgcaagcat catttcgcgc tcgccatttg gggtctcatc aaggctctct
    12601 tcaaacatgc gattcatcat gttgcgcaag gttgccattc ggcggtaggg ggaaatatag
    12661 aatgtcatgg attgtctcct cttttatatt ttgttagttt gtgtaggtcg gctttccaca
    12721 gccgccgggt cccaacgggc atgcaaaccc ggcctacatc attttttaga tgtcccgagt
    12781 ttaatggatg gatatcagag gagcacaaga gaaatataga atttttatta gaactgcaat
    12841 tggcgcatgg cattctcccc aggattcttt aggcaaatgc ctatcgcaag aaactgggat
    12901 agacagcaaa ctacttccgt cgggctgtga caatcaaata tggggcctgg tgagaatatg
    12961 gcgagcgatc gaagtcccca tataaagccg cctgtgacaa cccggctgcc tcgagctcgg
    13021 cgatgtagtc atctgtcgag agcagcgcgt gtcgcgcctg cattgagaag cgttccacgt
    13081 ttccgtcagg gagcaggtga tcgtaatccc atcggataac aaagtgccct gtggtgcgca
    13141 cccagctact gctcacctgc accggttcac cgtccagcgg gtggggaaag ctctcctcaa
    13201 cctccggctg gctccggcca agtaacttgc ggagcaaagt cgggttgggc aggctgacgg
    13261 caaaaagtcc ctgtggggct aagtggcgct gcacacaatc cagggtggcg cgccgcgcct
    13321 cagcggtgag cgtgctgaag gtgttacaag gcagcaaaat cagggcaaac tgctgtccca
    13381 attcaaaagc agccatatct gcctggatcg tgccaacccg ctcagccagt gcaggtggta
    13441 cattttcctg caatacgacg agcatatcgg ggtcactttc cagtccgagc acaaaatgcc
    13501 cggcttgtgc cagtggcaac aaaacacgac cactgccgca tcccagctcc aggatgggat
    13561 caccttgcag gcgcgccaga tctaaccaga aagggagatc ttcctggtga gcgttatggt
    13621 gggcgtgata gagacgagcg agcatcaatc tccttgacgt atcaggctga aacgcattat
    13681 actctctgag ccggggcgta gcgcagtccg gtagcgcacc gcgtttggga cgcgggggcc
    13741 ggcggttcga gtccgcccgc cccgactttg gttggcaatc atccctgtgt ggaggaaagc
    13801 cagtggcaag gatcaaattt gatggagttg tggaagctgt ccgctataaa cccagcgggg
    13861 agatagattg ggtgcgcgcc tacgaacgtc gcggcccaac cttcagcgat tacatcttac
    13921 ttgaccgcga gacattaatc aagcgcatta aagcaggcaa gcgctacgtc tccgggaaac
    13981 gcatccccta tatggccagc accttcgaaa cctcttcccc gattcgcctg gtgaccaata
    14041 gtggaaaaga gatcgtggtc accagtgatc cgaaggccac tcaggaccgc ttggagggcg
    14101 tgccaaccat ataactggtg atctattaac gaaaaacggt ttatagggca gcgttgccac
    14161 cgccctataa accgtttttt taatctttcc cttgcagggc gtgaacagta agctctactt
    14221 gatctcgaag acgatgttca catccaccat gagcaccaac tgaccgggag atacaggcac
    14281 tggtgcgccg gctccgcccc cgccatagcc cttggcatcg taaataggca ctggcgcacc
    14341 gccaccatag ctgttgatca tctggatatc tcccagggtt acaccagcgg cggaggccaa
    14401 ctctttggct gtagccagcg catcggctac tgccgacttg cgcgcttcgc ttagcgcttt
    14461 ggaccgatct gccgcatcga actggatgct gttaatcgag ttcgctcctg ctcctacagc
    14521 agcgccgatc acatcgccca ccttggagat atctcgcagg gtgacgaaaa cggtgttatc
    14581 gacaaaatag acaacccctg tgatcttccc tgtcgggtca tattgctgtt gagggaaaat
    14641 gctgaaattg gtggtctgga tgtctttggg gtcgatgttg aacttcttga ccgcatcggc
    14701 taccttcttc gagcgagcgt tgttgctggc tacggcttcc gcggcgtcag cattttcagt
    14761 gtgcacaccg atgttaatat aggcgatatc aggggtcaca aaagccttgc cactcccggt
    14821 tacgttcagc tggcgcgggg gagtttccgg ccccgggcct tgttgagtta ctgcaagaac
    14881 ctccgtacca ctgctgaagg cgctcaatag cgccgcaaca agcaacagag caattcctag
    14941 aaagactttc ttacgcatgg ggttatactc ctttgttatg atgtactagc acttagacgc
    15001 aaaacattgc gatagtgttc cgtgattttc gatcactttc atcataacgt caatccccca
    15061 aagaaagttc ccatacccca aagagtatca aaatagttag catattgtgt ccaatctctt
    15121 gttacaattg ataataatag gaatttggcc ctaatctgag ctacatcacc aacggccagt
    15181 gatcacagtt aatcattacc agaaattatc gcgtaatcat gtctgaaaat ctcacacccc
    15241 cacccccatc accccgtctg ttgatgctgt accgaatccg gcgcatgatg ccacgcattg
    15301 atcgtgggca gcgcgccgaa gtccaggtgc tgttgcgtaa cgcctcacac ccaaatttcg
    15361 atttcttcat cctggtagtg ctctcaagca tcattgccac gttgggctta ctcaccgatt
    15421 cgccagcggt gatcatcggg gcgatgctgg ttgcgccgct tatgtcgccc atcatcggtc
    15481 tgggattagc ttccctgacc gccgacggtc gcctgctggg ctccgccgca tcggccctca
    15541 tccggggtgc cctattggct atagtgattt ccttcctgat ctccgcaatt aaccgactgc
    15601 tgcctttgat tgccctacaa gcgcaggatc ttccgggaga ggtgctggct cgcacgcatc
    15661 cctcaccgat cgacttaggt atcgccctgg ctggcggcat ggcagcagcc ttcgccctgg
    15721 cgatgcccaa catctccgct gcgctccccg gggtggcgat tgccacagcg ctcatgccac
    15781 ccctttgcac ggttgggatt gggctggcct ggggacggca ggacatcacc gggggcgcct
    15841 tacttctctt cgtgactaat gcggtgacga tcgcctttgc agccatactg gtcttctctg
    15901 cgctgggctt cagcccgcgc cgcaaagaag gtgccgggct atttcctcgc agcttgcaga
    15961 tcgcagccct gctcacgctt atattgatga ttccactgac attattcagc gtccgcttcg
    16021 tccaagaggc tactagtgac cgaacaatcg cccaggtgat cgatgagaac gtgcgcgcct
    16081 tcggtgacgc cgagctagtc gagtgggatt cgaaccagga ggctggcacc ctaaaaatca
    16141 acatcaccat gaggacaaac aagcccttgc gcctcgaaga cagcatcgcg ttccagaaag
    16201 ctgtgggagg taatttacat gcccagggtg tcttgggcga caacgagaaa gtccaggtgg
    16261 ttatcaacca ggtgctggct gaacgactcg acccgctgat accgccaacg tctacacaca
    16321 cgccaacggt caccttcacc cccacacctg gaccgagcgc gacgccatca ttgacgccca
    16381 cccccaccgc cacctatacc ccctcgccca ccagttcgcc cacggcgact atcaccccca
    16441 ccgcaacccc cgtgcccacg gatacgccca ctccggcctt ggcgctgggc gccagtgcgt
    16501 cactcccctc tttccgcttg cggcaatcgc ctgaggggcc ggcgattggg ccagccctgc
    16561 gcacagggtc gccgctcacc gtgctctacg gggttcaaat cgtcgatggc ctggtatggg
    16621 tcgaggtaca ggattccgag gggcgagttg gctgggtgcc tcagatttac ctgaaactga
    16681 tcacccccac cccaaccaga acgccttcgc tgactccaac tgctttgaca cccacttcgt
    16741 cacctacccc tccatgaatc tcacatacgc agtgagagat gagcacgcag gggataactg
    16801 tcctgagatg cgaaagccat ctttatgcga acattttttc gaaattcata tacaactctt
    16861 atgcgccgcc aattcttcca caccctcaga atgttataat cagtgtggag ataaactcaa
    16921 acaagctgag tttgcgtttc tccaaaggaa ggaaaatagc ccatgatgag atttgatcgg
    16981 tttaccgagc gggcccaaga ggccgctcag cgtgccgcag agatcatcca gcggtacggc
    17041 cacaaccaaa ttgacactga acatatcctg ctggcactta tcgagcagcc gcagggtgtg
    17101 attccgcaaa ttttggagtt tctcaaagta gatgccgccg ccctgatcga gcggttagat
    17161 tacatattgc gcaccagccc caaagccaat attttcggcg gtggggccgg gcagatcttc
    17221 ataaccccac gcgtgaagcg catcatcgac ctggcgaacg aggaagccaa tcgtctcaaa
    17281 gacgagtaca tctctaccga gcacatcttt ctagcgatcc tgagcgagcg cagcacgcca
    17341 gccgctcgac tgcttgaagg ggccggcgtg acccgcgagc gcgtctacga tactgttcag
    17401 caaatccgtg gcgggcagcg tgtcaccgat ccgcaagctg agactcgcta ccgaacactg
    17461 gaaaaatact cgcgcgacct gacccagtct gcccgtgagg gcaagctaga cccggtcatt
    17521 gggagggatg ctgaaattct gcgcgtgatc cagattctct cccgccgcac caagaataac
    17581 cccgtgctga tcggcgaggc tggggtgggc aagaccgcca tcgtggaagg cttagcccag
    17641 aaaatcgcca gtaatgacgt gccggagatt ctcaccggca aacgcgtggt cgccctcgac
    17701 ctgggcgcga tgattgccgg ctcccgcttc cgcggggaat ttgaagagcg cttgaaagtt
    17761 gcgattgaag aagtgcaacg cgccgccggt gaggttatct tgttcatcga cgagctgcat
    17821 acagtggtgg gcgcaggcgc cgcacaaggc gctatggatg cctccaacat gctcaaacca
    17881 gccctggcac gcggtgagct gcagtgcgtt ggcgccacca cgctggacga gtaccacaag
    17941 cacatcgaaa aggacgccgc cctggagcgc cgctttgccc cggtctttgt ggaagagccc
    18001 aacgtggaag acaccatcca aatgctgcgc gggctgcgcg accgctacga agcacaccac
    18061 aaggtacgtt tctccgacga tgccctggta gccgcggcgc ggctctctgc ccgctacgtc
    18121 accgaccggc atctgccgga taaagccatc gacctaatgg acgaatcggc ggccaaattg
    18181 cgcgtggcgc tctactccct gccacccgag atcaaggatt taaaggtcga gatcgatcgc
    18241 ttggctgccg aagaggaaca atctggactg gagcgcgatt acgaacgcgc ggcgcagaaa
    18301 aaggcagagc gcctgcgatt ggaaactgaa tttaatgaaa agcgcgacca gtgggaagcc
    18361 gagcaccagc ttgacgaggt ggtggatgtc aatgacatcg cagaggttgt cgcacaatgg
    18421 acaggcatcc ctttgcacca gatgatggaa accgaggcgg agaaactgct acagatggag
    18481 gttcgcctac acgaacgcat caaagggcaa gacgaagcca tcaaggctat ctcggatgcc
    18541 atccgccgcg cccgctccgg tctgaaggac ccgcgcaggc caatcggctc gttcatcttc
    18601 atcggccctt caggggtcgg caagacagag ctggctagag cgctggcaag attcatgttc
    18661 gaagacgaag acgccctggt gcgcatcgac atgagcgaat accgagagca gcacactgtc
    18721 tcccgcctgt ttggtgcgcc tcctggatat gttgggtacg aggaaggagg ccagctcacc
    18781 gaggcagtac gccgccggcc ataccgcgtc atcctgttcg atgagatcga gaaagcccat
    18841 cctgaagtat ggaatgcgtt gctgcagatc ctggatgacg gccgcctgac tgatgggcag
    18901 ggtcatattg tggacttccg caacacggtt ttgatcatga ccagcaacct gggcactgag
    18961 tttgtgcgca aagcgggcag cttaggcttc ctgcaacgca aagacgacga tgaagagcgt
    19021 gccgcccacg agaagatcga aaaggcactc aagagcacat tccgccccga gttcctgaac
    19081 cgaatcgacg agattatcac tttctcgccg ctcagcctgg agcagatgcg cgaaatcgta
    19141 gacttgcaga tgaacgaggt gcaggagcgc ctgagcgagc atggcttggt ggtagagctg
    19201 agcgaagaag cccgtgtctg gctggccaat gtgggctacg atccatcttt tggtgcccgc
    19261 ccactgcgcc gagccttgca gaagcacgtg gaaagcccgc tttcggtcag cctgctcagt
    19321 ggcgagttct cgtcaggaga taagatcttg gtggaagtgg acgaggagaa gaacgagctt
    19381 accttctgcc gcgtggaaca ggcaagcgac caaagcatac cagcagaaga ggtcagccag
    19441 gtcgaggcct gataagaatt cttctcaacc ttggatgaaa caacatgagg ggttctgccg
    19501 gatcctattt atcctgcaga acccctcttt ttgcaactcc ttcaccccct gtctcatctc
    19561 cgtatgagca aaatttgcaa gtgattatga cgattgtcca agaaacatca ttacttttaa
    19621 tgatgtttat ccacagattc aggaagttcg ccactagaag ggcggggtaa atctggctac
    19681 agtatatgtt accctggagg tcaattgaac ggcatgtcga gtgatatctt cgatcaacta
    19741 ccgctatttg atggctttac ttctgaccag cgagaactgg tgcgccctct ttttaccccg
    19801 gtagactgtt actccggtac agtactattt gaacaaggcg atccggcaga ttatctctat
    19861 ctggtagttg tcggcgaggt cgtcatccgc tataagcctg aggacggccc tgaaatcctg
    19921 gtggcgcgtg tgcgtccctg gggtatcgta ggctggtcag ccgcgctggg gagtcatgcc
    19981 tacacctctg gggcaatatg tgaagaatac acccagatgc tgcgcctgta cggcaaagac
    20041 ctgcgtaacc tttgcgaaca aaatccggaa actggtatac ttatcctgga gcgcctggcg
    20101 actgtgattg cacagcgcct gcgcaacacg cacgagcatg tcatggaact gctcagacaa
    20161 ggattgggta ctaattcgaa ttacgcaaag gagggtagcc atggtcgatc ccgtctatga
    20221 agaccgatta cgcgccctga tcgagcggct aagcgcttat atcgagcatt accatggtgg
    20281 gtcggtacaa tatatatctc ttgatggctc ggtattaaaa gtgcgccttg gcggcgcctg
    20341 cctgggctgc ccgctcctgc cgagcacgct gcatggttgg gtggagggca ccgttcgcca
    20401 gtttttccct gaaatcgagc gcatcgaaga agtgtaacta cgcttaaaat acaacagcgg
    20461 acacaagtgc ccgctgttgt attttaagca tctatgcccc ggactcagcc tcacctcccg
    20521 cctcttcagg tgagacgata gggttgcgag agatgtaatc gtccacaaag agcacgttgt
    20581 ccttggaacg gcgcacaata ctcaacagac gatccatggt agagatctct tccaattgct
    20641 cggtcacgaa ccagcgcagg aagtcttgtg cgatatggtc tttttgctgg atggccagat
    20701 tcatcaactc gttgatctgg cgcgtcactt cctgctccca gtccagggcg agctggacag
    20761 cctcttcagc agacgagaaa tcatacttgc tggcagagat ggccggtatg cgtacctgac
    20821 cgccggcatc cacgacatag ttcagaaact tcattgcatg catgtgctct tcactggctt
    20881 gccggtagaa aaaggctgcc aggctgggca ggttatctgc atcaaagtag gcggcgatgc
    20941 tattgtattg caaactggcg ccaaactcgc gcccgacctg tgtattgata gcttcctcta
    21001 attctttact gattaacatg ttatccttcc aattctttga accaatcatt gtgtacgcag
    21061 gtggggagtt ttttccctca actgcgttag tattataaat atcacggcga gattatatca
    21121 cgcgctctgc agggttataa gatgcctgtg ttcctactca cacactcatg gcgcagccat
    21181 cagcccgcgg atcacttcca ccccacagca caccgctctc agcatcccgc aagatgatct
    21241 ggccgcgccc aaagagcgcc cgatcatagc ccgccaccat ctcgaccggg tgtcccctca
    21301 aggccaattc gcttttaacc tgaagggaga tgccttcttc taaggcgacg cccccacctg
    21361 cctcgccgtc ttggatgcag aaacgtggct ggtctagggc agcttgcggg tccagcttgt
    21421 catccgccag ggctacaagc acctgcatat gtccctgtgg ctgcatgaaa ccgcccatca
    21481 cgccaaagct ggcgacaagt tggtggttgg cttgtgcagg ccggcggtct actgtggatg
    21541 attgctctct atccactgta atcatgcctg ggatgatggt gtgataaggc cgcttgccgg
    21601 gcgccagggc attgggatga cctgcgttca ggccgaaatt atgcccgcga ttctgcaggc
    21661 tgaatcccca gcctgtcggc acaatgccag tgccaaatcc catgtaattg ctgttgatga
    21721 atgagcaggc gttcccctcc ccatctacca cgctcaaata gactgtatcc gaggcggctg
    21781 tgggcgtgcc cgtctggaat tcaagatttg cgcgggctgg gtcgatcaga cgacggcgcg
    21841 aggcagcgta atccttggag agcagctcgt ccacaggtgc tggattgaag gccgggtccg
    21901 ccacatacca gcgggtatcg gcaaaagcca ggcgcatagc ctcgatctgc aagtgcaggc
    21961 gagtggcaga gagcggctcc atagaggcga gatcatatcc ctccaggatg ttcaatgcga
    22021 tcaatgccgc cagcccctga ccgttcggcg ggcattccca aacacgcagc ccgcggtagg
    22081 tcgtatggat tggttgctcc caggtgctct cgtgcgcagc caagtcgtca gttgtcaggc
    22141 aacccccggc tgcctgcacc gttgcagcga tggcttcagc gatggcgccc tggtaaaagg
    22201 cttgctttcc agcctgggct acagcccgaa aagtgcgcgc caaaccgggg ttgcggaata
    22261 gctctcctgg acggggggcg cgaccctcaa tggtcagctc cagcccgccc ggggcatgac
    22321 caagctggcg tgtaacacct cggtcccaaa aatatgctgt gaccggcgtc accgggaaac
    22381 cctcatccgc cagtcggatg gcaggggtga ggacatcttt caataaaagc cgcccgtggc
    22441 gctcgaccag gtcgcaccaa ccagcacagg cgccaggcac ggttatggta tagggatgat
    22501 acggcggcag ctcggtccca aaaccttcgc gctgcagacg ctccagggtc agggcagccg
    22561 gggcgcgacc agacccgttc atcgcgctca cctggcaggt agcggcgtca tagtacaaag
    22621 cgaagcaatc cccaccgatg ccggtggaag ttggctcggt gacgttgaga gcagcggcaa
    22681 cagccaccgc ggcatcggca gcattaccgc ccttgctcag gatttccaga ccagcagcca
    22741 ccgccagagg ttgggaggtg gcgaccatgc cacctcgtcc caacaccgga gaccggcgag
    22801 agttgaattc gtagccgcca ttgccaaaca ttctatgtat aggcattaaa tacccgctct
    22861 tctccccgtt tgaggggatt tcgtaaattt atcgtttgcc acgcttggga atttattttg
    22921 taattccacc catcttcggg acaccatcgt gcccgcaggg ttcgataaca tctgcgcctt
    22981 atcttagcac ctcaatgatc tccccatcca cgacctcccc cacctcccac aacacctgcc
    23041 cgacctgggc agcgcggctt aataagccct ccagtttctg gcgtggcacg cagagtagca
    23101 agccgccgct ggtctgcgca tcgaacagca gcatctggga tggctcgtcg attccttcgt
    23161 caaagcgcac atggtcgcca tagtacaggc ggttatccgc ggagccacca gggaatatac
    23221 cctcatgggc ataacggcgc gccccgcgcg tgaaggggat tttcccgaag aaaaagcgca
    23281 gccccacgcc agaagcctca gccatctccc acccgtgacc caacaggctg aatccagtga
    23341 catcagtacc accgcgcacg cccgcctcga tcgccagctc agaagcagct ttattcaggc
    23401 gcttcatcca ccccaccacc tcagatacat ccgtgggatc agcctgaccg cgcttcaagg
    23461 ctgtggtcgt agtgccaaag ccaagcggtt tgctcaaaac aagcacatca ccctggcgag
    23521 ctccgctttt cgtgatcagg cgctccggat cgacaaagcc caccacaatc aggccatatt
    23581 taggctcctt atcctgaaca gtgtgcccac cagcaatgac cactcctgcc tgccgagcga
    23641 cctccgcccc gccgcgcaga atctccccca gcagttcagc aggcagatca ggcggcaggg
    23701 ctgccacgtt caatgccagg aagggttgcc cccccatggc atagatatcc gaaagggcat
    23761 tggctgcggc gatcgccccg tagtcatagg catcgtccac caccggggtg aagaaatccg
    23821 tcgtaacgac cagggcgcgg cgttcatcca gacgccacac ggcggcgtcg tctggatcgg
    23881 acaaccccac caagaggtca ggataatcgg cgggattaaa tatgtcctta atcgggcgca
    23941 gcacctgcgc cagcgcttcg gcgcttagct tcgatgctca accggcgcaa ctcgaaaggc
    24001 tggtcaggcg gatctgacgc ccagagggat tgaaatggct catctttaca gatcagaagt
    24061 tacgcaattg cggaggtact tctccttcaa ctgtgcacgt attttagaat ataggtcacg
    24121 gtgttgtcgc tgggggagag ggcgacggtg acggcgacgg ctcgaccacc cccggctcca
    24181 gggtgggcag tggcagtagg aaggggttgt cgctgggcac cagcatgacc tgcaccccgg
    24241 gagccagccg gttgatatac tggtaggtga tcagctcggg attatcctgc agggcgtcgg
    24301 cgatgagcaa caaagctttg gcctcggcgt cagcctggat caagcgcgca tcggcgtcac
    24361 ctttggcgcg gatcactgcc gcgtcagctt gaccctgggc agactggcgc gcctgctcgg
    24421 cttcctgctt tctctgctct accaccaacc gggcctgctg ggcctgctgc tcggcaatct
    24481 gcttctgctc caccgaggcg gcgtattccg gcgagaaggt gatattgcgc agcacgaagt
    24541 ccaccagaat catcccattc tcatctaact tggctgacat gtttgtccgg atctgatcga
    24601 ccagctcaaa acgcttcgag gtgaccacct cttcgacacg aaactgcgag acggcatcgc
    24661 ggatgatacc gcgcgatagg ggccgcacca ggtcgtcgat gtagcggttt tgccagcgga
    24721 tatgcaactg caccacctct tcaggatcaa cggcgtatag caccgaggca tctacaaaga
    24781 tctcctgccc atcggctgtg cgggcagcca ccgagtcatc accctgcacc tggccctcca
    24841 aaggggcaat ggacatggtg taggtttgtc tggagatcgg gtatgtgacc acgttctcca
    24901 caaaggggac aacccagcgc agaccggctt ggagcgcctg cgggcgatag cctcctgggt
    24961 ctaaggcggt gatcaccaca ccgcgctctt gaggcgagac gaacaccaga ccagcactgg
    25021 ctgtggagag cagcaatgcc aaaacgccca caacgatgat aatggtcgtt gcgcctcgaa
    25081 gaggctggcg gcgagaggcg cgccctacag cgagggcgat gatgcccaga aatacgaacc
    25141 aggacaggct ggcaataatc ttaatggtac tagctacatt catagataaa acctcctata
    25201 ctcgaacggg taaacgatct cattatactc gtgtttcttc acctctgatg ctctgaggct
    25261 cagggcaatc gcatttgggc gatttcccac cactgaggag agaaaatggg gtgttttggt
    25321 gggggcatac ccccaccaaa acaccccaaa cactcttttt gcgggattgc gcatgcccct
    25381 cagggcatat gcatatgccc tgctctgagg ccacaccatt gcctgccagg acaaaatgtg
    25441 ctaccatgcg cccgtgcgcg cccaaactgc ccgtgtgata gagatccaac aagaccctgg
    25501 gggtttttca gcttggctca cctgccccgc gcaggtcatc ccagcagccg ggcaatatgc
    25561 cctggctata gactccaaag atgcgcttca acccctctcc atgccgctct tccctatggc
    25621 ttactcaaag gacagttttc tcgccgcctc gccttaccag atagcatgga cgcctgggac
    25681 agccctggag attcatggac cgctgggaca cggttttaaa ctacctccca ccgcacgcaa
    25741 tgttgccctg gttggccttg gggagacctc ggctcgcctg atgccgctca tccccact
//
LOCUS       DBNGSX010000425         5630 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1562850, whole genome shotgun sequence.
ACCESSION   DBNGSX010000425 DBNGSX010000000
VERSION     DBNGSX010000425.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5630)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5630
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1562850"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="AC16CB_12640"
     CDS             <1..574
                     /locus_tag="AC16CB_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR3343362.1"
                     /translation="DIVIEMLHKVGMEDYHARRYPHEFSGGQRQRIGIARALALRPKF
                     IIADEPVSALDVSIQSQVLNILKDLQSEFGLTYLFIAHNLSVVEHISDRVAVMYLGKM
                     VEMADRDELFRNPLHPYTQALMSAIPIPDPTLKRERIILQGDVPSPLNPPSGCRFHPR
                     CPVAFEHCKVQEPPFKEVSTDHWVACWRIE"
     gene            589..1626
                     /locus_tag="AC16CB_12645"
     CDS             589..1626
                     /locus_tag="AC16CB_12645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3343363.1"
                     /translation="MPSFRVLIVDNQRSVRRSLRSGLESTGQDFEVLEATSGEEAMLA
                     FASLPVDLLVTGVRLAGMSGLELLQRMRQNRPELKGILTAVNPDTRTGQLALKANANA
                     LVPKPIDISKFLEAVYRCLDQEPERGLPPKQQATSAGSLSERLEAIYHRCGAMAILLL
                     DEDGHILAQAGEAEKAKPLMSQIPVVLEALGASARLSQAMGENIPDDLVCCAGLGVEL
                     FAAHLGKLNALLYIFDTGVLGYTRARSVRLVSESVLEMTGFMPMFGGAQRSMLAKKEE
                     PTDPRPKKEAETNVDLDEVFQQASKNSLDPQDVDGFWETAVDQPDSDSGAGPDSISYD
                     QAKDMGLVQDG"
     gene            1694..1764
                     /locus_tag="AC16CB_12650"
     tRNA            1694..1764
                     /locus_tag="AC16CB_12650"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1726..1728,aa:Gln,seq:ttg)
     gene            1912..3258
                     /gene="glmU"
                     /locus_tag="AC16CB_12655"
     CDS             1912..3258
                     /gene="glmU"
                     /locus_tag="AC16CB_12655"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HIR3343364.1"
                     /translation="MNISAVILAAGQGTRMRSSMPKVLHPLLGSPMLLYSLEAARLVT
                     GTTPVLVIGHGGEKVKQVIGGAAHYALQEPQLGTAHALMQAEPLLRGKADLVLLTAAD
                     MPLITPETLSKLVQAQAAHAGPITLLSGRADTASDFGRVLRGPDGRIQAVVEVAQATP
                     EQLAVREVNTSIYCFAAEWLWDALHRIPLSPKGEYYLTDAISLAVAEGLSVQGVQVED
                     SIETLGINTRVQLAEAEVALRQRINRGWMLDGVTIVDPATTYIEPGVHIGTDTTIWPN
                     TYLQGNTEIGENSVIGPNAVLRNTRVGDGCKVLASVLEDAVLEDDVDVGPFGHLRRGA
                     HLAQGVHMGNFGEVKDSYLGPGTKMGHFSYVGNATIGPKVNIGAGTVTCNYDGEKKSP
                     TEIGAGAFIGSDTMLVAPIKIGEGARTGAGAVVTKDVPPNTLAVGMPARRIKKLRK"
     gene            3265..4548
                     /locus_tag="AC16CB_12660"
     CDS             3265..4548
                     /locus_tag="AC16CB_12660"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR3343365.1"
                     /translation="MIDIGVLTAGFVIILVLDFITVASGTALQKTNMARLLAYSEAGD
                     PGVQGAMRLLNSPARLQATIDLAQWIWRALQVLLLFIILYQGPWISPLAAAAGLTLLA
                     SLVIFWLEWYIRESTGKSAEAWTLRLYPFIAVLLAVFSPFVALPLAVSGGEQEQENGG
                     SMVDELKSLVDAGQRDGLLEQEERRMLRSIFELGNTLAREIMVPRIDILALDVHTNLP
                     DALDVLLNTGHTRVPVYQDTVDNILGLLYAKDLLRVWREGDTTGSLRNLLRPAYFVPE
                     AKKVDELLAEMQSRRVHMAMVVDEYGGIAGLVTMEDIVEEIVGEIQDEYDLAEELPYN
                     ELSDGEYLFMGRVDLEDLNEVLGTHLTRDEADTLGGLIYSRLGRVPVSGESVQVDDLL
                     LTIEQVSGRRIRKVRVQREAPSSPDNEEPMSHANG"
     gene            4535..4933
                     /gene="cdd"
                     /locus_tag="AC16CB_12665"
     CDS             4535..4933
                     /gene="cdd"
                     /locus_tag="AC16CB_12665"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020151729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HIR3343366.1"
                     /translation="MLTDELRTKLIEMAIEARKWAYAPYSNYKVGAALLTTSGRIYDG
                     VNVENAAYPATICAERAAVVKAVSEGERNFVAMAVITANGGFPCGYCRQVLAEFGLDT
                     TVIIADSEGKVYREATVGELLPEAFGPSFL"
     gene            complement(5034..>5630)
                     /locus_tag="AC16CB_12670"
     CDS             complement(5034..>5630)
                     /locus_tag="AC16CB_12670"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3343367.1"
                     /translation="LKYRMNLEKESDLVAGTVFIVALLTLPFWERASRRWDKRIAYIA
                     GMIFLSSVIITLIVISPSWGLTVVFTLAGLAGIGVGAVHVLPWSMIPDAIEWDELETG
                     QRHEGMFYSLVTLFRKVSSSISLPLILLVLDWSGYVSNAAVQNPQAVQAIRILMGPVP
                     SVLLCAGIAFALLYPLSRDRHTQMRAEIAARALKSLSD"
BASE COUNT         1261 a         1431 c         1633 g         1305 t
ORIGIN      
        1 cgatattgtc atcgagatgc ttcacaaagt ggggatggaa gactaccatg cacgccgcta
       61 tccgcacgaa ttctccggag gtcaacgcca gcgcattggt attgctcgcg ccctggcgtt
      121 gcgtccaaag tttattattg ccgacgagcc ggtatctgcc ctggatgtct ctatccagtc
      181 tcaggtgctc aatatcctta aggacctgca gtcagagttt ggcttgacct acttgtttat
      241 cgcccacaat ctgagcgtcg tcgagcacat ctcggaccga gtagcagtga tgtatctggg
      301 taaaatggtc gaaatggccg atcgtgatga gttattccgc aacccgctgc atccttatac
      361 ccaggccttg atgtcggcta tcccgatccc cgatcctact ctaaagcgcg agcgtatcat
      421 ccttcaaggc gatgtgccaa gcccgctcaa tccgccttct ggatgccgct tccacccgcg
      481 ctgcccggtt gcttttgagc actgcaaggt gcaggagcca cccttcaaag aggtcagcac
      541 cgatcattgg gtggcttgct ggcgcatcga gtaaaaaaac cctttatcat gccatctttc
      601 cgcgtcttga tcgttgacaa ccagcgcagc gtacgccgtt cactgcgctc tggtttggag
      661 tcaaccgggc aagatttcga ggtactggag gctacatcgg gggaagaagc catgctagcc
      721 ttcgctagct tgccggtgga tttattagtc accggtgtgc gcttggcggg tatgtctggc
      781 ctggaattgt tgcaaagaat gcggcaaaat cgcccggaac tcaagggcat cttgacagcg
      841 gtgaacccgg atactcgcac cggtcagctt gcgcttaagg caaacgccaa cgctttggta
      901 cccaaaccaa tagacatttc caagtttttg gaagctgtgt accgttgtct ggatcaagag
      961 cccgagagag gattgccgcc aaagcagcag gctacctctg ctggcagcct ttctgaaaga
     1021 ctcgaggcta tctatcatag atgcggtgcg atggcaatcc tcttgctgga tgaggatggt
     1081 catatcctgg cccaggctgg agaagcagaa aaagccaagc cgttaatgtc gcaaatcccc
     1141 gttgtgctgg aagccctggg tgccagcgcc cggctatctc aagccatggg tgagaacatc
     1201 ccggatgact tggtatgttg tgccggtcta ggtgttgagc tgtttgctgc gcatttggga
     1261 aagttgaatg cgttattgta tatttttgat acgggcgtgc tgggttacac acgcgctcgc
     1321 tcggttcgtc tggtgagcga gtcagtcttg gagatgaccg gttttatgcc aatgttcgga
     1381 ggtgcacaac ggtcaatgct ggcgaagaag gaagaaccca ctgatccccg gcctaaaaag
     1441 gaagcagaga ccaacgtaga tcttgatgag gtcttccaac aggcatccaa gaatagcctc
     1501 gatccacaag atgtggatgg cttttgggaa accgcggtgg atcaaccgga tagcgattcg
     1561 ggtgctggac ccgattcgat ctcctacgat caagccaagg acatgggtct cgttcaggat
     1621 gggtaagctg attcgggaca ttggcatctc taacttatga tacaatacgc ttcgcctgtt
     1681 acccaggtga ttttggggcg tggtgcaatg gtagcacagc ggactttgaa tccgtatatc
     1741 ctggttcgaa tccgggcgcc ccagtctgat cgttttgcac aggttttgtt tcgtgtctga
     1801 cgaagagaca gtttcagacc acagatttgg tttaaccttt cagcgagccc cgcgctacca
     1861 taattggatc gtgggtgctc gttgttttta ttctattccc atggagttct catgaatatc
     1921 agtgctgtca tcctcgccgc gggtcagggg acgcgtatgc gttcgtcgat gcccaaagta
     1981 cttcacccat tattagggag tccaatgttg ctctattcgc tggaagccgc ccggcttgtc
     2041 acagggacta cacccgtact ggttattggt cacggtgggg aaaaggtgaa gcaggtcatc
     2101 ggtggggcag cgcactacgc tttacaggaa ccgcaattgg gcaccgcgca tgctctgatg
     2161 caggctgagc ctctcctgcg tggcaaagct gacctggtat tgttgactgc cgccgatatg
     2221 cccctaatta ctcccgagac gttgagcaag ctcgttcaag cgcaggcggc tcatgcaggc
     2281 cccattactt tgctctcagg acgagcagat acagccagcg actttggtcg ggtattgcgc
     2341 gggccagatg ggcgcatcca agcggttgta gaagtggcgc aggctacacc tgagcagctt
     2401 gcggttcgcg aggtaaatac cagcatctac tgctttgctg ctgagtggct ttgggatgcc
     2461 cttcatcgta tcccgctctc ccccaaagga gaatactatc tcaccgacgc gattagcctg
     2521 gctgtagcag aaggattgag cgtacaagga gtgcaggttg aagacagcat cgagaccctc
     2581 ggaatcaaca cccgggtaca attggcagaa gcggaagtcg ccttgcgcca acgtattaac
     2641 cggggttgga tgctggatgg ggtgaccatc gtcgaccctg caactaccta tattgagccg
     2701 ggcgtgcata ttggaacaga tactacgatc tggccaaata cttaccttca gggcaataca
     2761 gagatcggtg agaacagcgt gattgggcca aatgctgtat tgcgcaacac ccgggttggt
     2821 gatggctgca aagtgctggc ctcggttctg gaggatgcag tcctcgaaga tgatgtcgat
     2881 gtgggtccat tcggtcatct gcgcaggggt gctcacctag ctcagggtgt gcatatgggc
     2941 aatttcgggg aggtgaagga ttcatacctg ggtccgggaa ccaagatggg gcatttttct
     3001 tatgttggca acgccaccat tggcccaaag gtgaacatcg gtgcggggac ggtcacctgc
     3061 aactacgatg gcgagaagaa gagtccgact gagatcggtg ccggcgcctt tatcggcagt
     3121 gataccatgt tagtggcacc gattaagatt ggggaagggg cgcgcactgg cgctggtgcg
     3181 gtggtcacca aagatgtgcc gcccaatacg ctggcggtgg gtatgccggc tcgcaggatc
     3241 aaaaagttaa ggaagtaggg aattgtgatt gatattggag tgctcactgc tggttttgtc
     3301 attatattag tgctggactt catcacggta gcttctggga ctgccttgca gaaaaccaat
     3361 atggcgcgcc tgctggcata tagcgaggcg ggtgatcccg gagttcaggg tgctatgcgc
     3421 ttgctgaact ctccagcgcg actacaagcc accattgatc ttgctcaatg gatttggcgt
     3481 gccctgcagg tactgctgct gttcattatc ctttatcaag gaccttggat ttcacccctg
     3541 gccgcggccg ctgggctcac cttgctggcc tctctggtga tcttctggct ggaatggtac
     3601 atcagagaat cgactggcaa atcggctgaa gcctggacgc ttcggctata tccattcatc
     3661 gccgtgctgc tagctgtgtt ttcgccgttt gtggctctac cgcttgcagt ctctggaggg
     3721 gagcaggagc aggaaaacgg aggtagcatg gtggatgagc ttaagtcctt ggtagacgcg
     3781 ggtcagcgcg atggcctgct ggagcaggaa gagcgtcgca tgcttcgctc catcttcgag
     3841 ctgggcaata ccctggcgcg cgaaatcatg gtgccgcgca tcgatatcct ggcgctggac
     3901 gtgcatacca acctaccaga tgcactggat gttctgctga atacaggcca tactcgcgtg
     3961 ccagtttacc aagatacagt tgacaacatc ttgggcttgc tctacgccaa ggatttgctg
     4021 cgtgtctggc gggagggtga caccacgggg tcgctaagga atctgctccg cccagcctat
     4081 tttgtgccag aggcgaagaa ggtggacgag ctcctggcgg agatgcagag ccggcgcgtc
     4141 cacatggcta tggtggtgga tgaatacggt ggtattgctg gcctggtcac catggaagat
     4201 atcgttgagg agatcgtggg tgagattcag gacgaatacg acctggcaga agaattgccg
     4261 tataatgagc tgagcgatgg cgagtacctt tttatggggc gcgttgacct ggaagatctc
     4321 aacgaagtgc taggtaccca tctaaccagg gacgaggccg atactcttgg cggactgata
     4381 tacagccgcc tgggccgtgt ccctgttagt ggagagagcg tgcaggtgga tgatctcctc
     4441 ctgactatag agcaggtgag cgggcgacgc atccgcaaag ttcgtgtgca gcgggaagcg
     4501 ccctcgtctc cagacaacga agagccaatg tcccatgcta acggatgaat tacgaacaaa
     4561 gttaatcgag atggcgatcg aggcccgtaa atgggcatac gccccctact ctaactataa
     4621 ggtgggtgca gccttgctaa ccacatcagg ccgcatttat gatggggtca atgtcgagaa
     4681 cgctgcctac ccggcgacca tctgcgcgga gcgagcagcg gtcgtgaaag ccgtatccga
     4741 gggagaacga aattttgttg cgatggcggt aatcaccgcc aacggcggtt tcccctgtgg
     4801 atactgtcgc caagttttgg ctgagtttgg gctagatacg acggtgatta tcgctgattc
     4861 cgaggggaaa gtatatcgag aggccaccgt gggggagtta ttgccagagg cgttcgggcc
     4921 ttcttttcta tagaaacaag aaatcccatc ccccccttga tatagccccg gcctcgccaa
     4981 taggggttct attaacctgc tttcaaacaa gggtttaatg acctgcaccc agatcaatcc
     5041 gatagagatt ttagcgcgcg ggcagcgatc tcggcgcgca tctgtgtatg gcggtcgcgg
     5101 ctgagcggat ataacagggc aaatgcaatg cccgcacaca ggagcacaga aggaacaggg
     5161 cccattaaaa tgcggattgc ctgcactgcc tgggggtttt ggacagccgc attagataca
     5221 tagccagacc aatctaagac caacaagata agcggcagcg aaatggaaga cgataccttc
     5281 cgaaatagcg tcacaaggct gtagaacatg ccctcgtgtc gctgaccagt ttctaactca
     5341 tcccactcaa tcgcatctgg tatcatcgac cagggcagga cgtgcacggc acccacccct
     5401 attccggcca aaccagccag tgtgaaaaca acggttagcc cccacgatgg gctgataacg
     5461 attaatgtga tgatcaccga tgaaaggaat atcatcccgg cgatataggc aatgcgttta
     5521 tcccagcgcc gcgaggctcg ctcccaaaag ggcagggtta gcaaggcgac gataaacact
     5581 gtcccggcca ccaggtcgct ttctttctct aggttcatgc ggtacttgag
//
LOCUS       DBNGSX010000426         1870 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1146863, whole genome shotgun sequence.
ACCESSION   DBNGSX010000426 DBNGSX010000000
VERSION     DBNGSX010000426.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1146863"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1472
                     /gene="htpG"
                     /locus_tag="AC16CB_12675"
     CDS             <1..1472
                     /gene="htpG"
                     /locus_tag="AC16CB_12675"
                     /inference="COORDINATES: protein motif:HMM:NF003555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HIR3343368.1"
                     /translation="PQAKATTWYATGEDNFEVGLGDKADRGTLIEIKLKDDAAEFADE
                     SRLREIIHKHSDYVNFPIYIGEGEGQVNKTSAIWRMPKQDVPEEQYKEFYRQLTLDFE
                     EPLMHIHVIADAPVQVYALLYVPAQAERGILSLRRDDGLKLYSKNILINEYSKDLLPE
                     HMRFVQGVVDSEDLPLNVSRETIQSTGMMARLKKILTGQVIKELEDLSKKDAEKYHTF
                     WQEFGTFLKQGVATDQVEGEKLVPLLRFKTNLHAESWSSLDDYVGRVQDDQKVIYYIV
                     GDDPKSILHSPHLDYFQEQGVEVLLLPDPIDNFMLMGLRKYKDFELKNVALAAPETPE
                     KQEDQPDDEQRLSGDDVDQLIERFKRQLGERVADVRSTNRLSRSVARLVDPQGSLNPE
                     LQRVYKYLEKDYEVPKKILELNPSHAILRNLLTLDPEADLHKIIVEQIYESALLVEGL
                     HPDPSSMVSRVQQIIEAALSGISPDAQKQSLDESEDHQD"
BASE COUNT          500 a          438 c          486 g          446 t
ORIGIN      
        1 aaccccaagc caaggcgacc acgtggtatg cgaccggcga ggacaatttc gaggttggac
       61 tgggggacaa ggcagatcga ggtacgctga ttgaaatcaa gctcaaagac gatgctgccg
      121 agtttgcgga cgagtctcgc ctgagagaga tcatccacaa gcactcggat tacgttaact
      181 tcccaatcta tatcggtgag ggcgaggggc aggtcaacaa aacgtccgcc atctggcgga
      241 tgcccaaaca ggatgtgccc gaagagcagt ataaagaatt ctaccggcag ttgaccttgg
      301 atttcgaaga gcctctcatg cacatccacg tcatcgccga tgcacctgtc caggtatatg
      361 ccttgttgta tgtccccgct caagccgagc gcggcatcct gtcgctacgc agggacgatg
      421 gattaaagtt gtactcgaag aatatcttga tcaatgagta ttccaaggat ttattgccag
      481 agcatatgcg cttcgttcaa ggggtggtgg attcggaaga cctgccattg aatgtctccc
      541 gcgagacgat acagtccacc ggcatgatgg cgcgcctgaa gaagattttg accggacagg
      601 taatcaagga attggaagat ctgtcaaaga aagatgcgga aaaataccat acattctggc
      661 aggaatttgg gacctttctc aaacagggcg tggctactga tcaggtagaa ggggaaaagc
      721 tggtcccatt gctgcgtttc aaaaccaatt tgcatgcgga gagctggtca tcattggacg
      781 attacgtggg gcgcgtgcaa gatgatcaaa aagtgatcta ctacattgtc ggtgacgatc
      841 cgaagtctat attacacagc ccgcacttag attacttcca ggagcaaggt gtcgaggtgt
      901 tattgcttcc ggaccctata gataatttca tgctgatggg attgcgtaaa tataaggatt
      961 tcgagcttaa aaatgtagcc ctggcggcgc ccgaaacgcc tgaaaaacaa gaagatcaac
     1021 cagatgatga gcagagatta tccggtgacg atgtcgatca attgatcgaa aggttcaagc
     1081 gacaattggg ggagcgcgtt gcggatgtgc gcagcacaaa caggctctcg cgctccgtgg
     1141 ctcgcctggt tgacccgcag ggcagtctca acccggaatt gcagcgtgtc tacaagtatc
     1201 tggagaagga ttatgaagtc cccaagaaaa tcctggaact gaatccctcc cacgctattt
     1261 tgaggaattt gctcaccctg gatcccgagg cggatttgca caagataata gtcgagcaga
     1321 tttatgagag tgctttgctg gtcgagggcc ttcatcccga tccgtccagc atggtgtcgc
     1381 gtgtgcaaca gattatagag gccgcattgt ctgggatttc tcctgatgca cagaaacaat
     1441 ctcttgacga atcggaagat catcaggact agacaaaaaa ctgaatgtaa tttcaatagg
     1501 gttttgggag gcggtaccgc ctcctaatac cctgtttttc tgtatatccc ccctaaagtt
     1561 cgcgtaaata catgataaga ttatttatac gtatggttat tgagatgcga atctgtatct
     1621 taatgccata ctcttcattg aacccctaat tccatatact tactccaggt gtaggattcg
     1681 tcaggcgtga cggcgcctgt gtcgccatct acggccaaaa gaatgaaaga cagcttactt
     1741 ttaacgaaac tctatatccc gtcactaagt cccagggttg tgcaccgtcc acaccttatc
     1801 tcgcgtttga acgaaggttt agcgaggggc tgcaaactca ccctgctatc cgcgccagtg
     1861 ggatatggca
//
LOCUS       DBNGSX010000427         7796 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_940363, whole genome shotgun sequence.
ACCESSION   DBNGSX010000427 DBNGSX010000000
VERSION     DBNGSX010000427.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7796)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7796)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7796
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_940363"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1109
                     /gene="meaB"
                     /locus_tag="AC16CB_12680"
     CDS             93..1109
                     /gene="meaB"
                     /locus_tag="AC16CB_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HIR3343369.1"
                     /translation="MGVVDSVLEGNRLSLARLLTQIENGVPEGRAAMEALYPHTGKAH
                     LIGVTGAPGTGKSSLVNQLARLFRYPPSEDSPQRVGVVAVDPSSPFTGGAILGDRVRM
                     RDLAGDPGVFIRSMASRGTLGGLATATASMVLALDAAGFEVILIETVGAGQAEVDIAR
                     LAHTTLVVEAPGLGDDIQAIKAGILEIADILVVNKADRPGVENTERALRGMLQLGHPA
                     PRVYLHHGQTERLTPPADLEADNLLWQTPVVRTVATEGTGVDGLYQEIARHRRYLHES
                     GEWERRERARLQSELDILLQAALVSRWRETVSQEAYRGTVDRLVRRDISPWQAVESLL
                     DGHG"
     gene            1138..1674
                     /locus_tag="AC16CB_12685"
     CDS             1138..1674
                     /locus_tag="AC16CB_12685"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3343370.1"
                     /translation="MIYGERIRLRAPERDDLPRFVGWLNDPEVRQNLFMFLPLSMAEE
                     EHWFEDVLKQPAAERPMVIEILDGESWKPVGNCGFIHIDWRCRAGEVGIFIGDKSRWN
                     QGYGTEAMRLLLRHGFNTLNLNRIFLRVFDTNARAMRTYEKAGFVHEGRLRQAEYQDG
                     KYIDMLVMSVLYSEWKEN"
     gene            1689..2681
                     /locus_tag="AC16CB_12690"
     CDS             1689..2681
                     /locus_tag="AC16CB_12690"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIR3343371.1"
                     /translation="MLTHYLHRDTNMYGDIKLFAGTACPELAGTIAKYLELPLSGRDV
                     TLFPNDNLFIRLHKSVRGQDVYVIQTTSRPVHRNLMELLIMLQTLRLDSAGRVTAVVP
                     YMCYARSDKKDKPRVPITARLVADMIEIAGADRYMTLDLHAGQIQGFFSIPGDVLTAF
                     HILTAHVEKMLPALKDPVVVAADLGFAKKGRNFAATIQAPIAFIEKRRSAKEDQPIAL
                     TIIGDVKDRDVIIIDDEVDTARSVEQAVNLVKAHGARNVYLIFVHAVLSPPAMQRIAQ
                     LPLKEIITTDTIPIPSDQRALIQDRLTILSVAPLLGEVILRAHEGRSVGELFDE"
     gene            2685..3521
                     /gene="mutM"
                     /locus_tag="AC16CB_12695"
     CDS             2685..3521
                     /gene="mutM"
                     /locus_tag="AC16CB_12695"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HIR3343372.1"
                     /translation="MPELPEVETIAANLREGNPNQPSLIGKSIQQAHLLWERTLATPS
                     ASEFYSWIPGQSILDIGRRGKFIVFELSRHTLLIHLRMSGDLLVEPVDAVIAPHHRLV
                     IDLDGGIRLAFNDARKFGRVWLTENPSGVLAALGPEPLDESFRPLDLHQRLQAHRRQL
                     KPLLLDQSFLAGLGNIYADEALHLAGIHPLTQSNMLSLEKSERLWQGIREVLQEGILR
                     NGASIDWVYRGGDFQNYFRVYQRTGNPCYNCGTPIERLVVGQRGTHFCPNCQPFDGRR
                     HS"
     gene            3518..4057
                     /locus_tag="AC16CB_12700"
     CDS             3518..4057
                     /locus_tag="AC16CB_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343373.1"
                     /translation="MNANIMLIVVGLIGIAIGFALGVALMSLRSPQAEENEKEPPPLQ
                     KRIQTPPPKAPPTVDVAPRPVSKPTPSLEDSPPPQQRPTIKPANVLARVLQPDLSAAQ
                     APPKSIAAQIDEILQEMLEDSPLDSRGIRLMEFPNRGLVVMVGLDQYEGVDAVPDEDI
                     RRLIRSAVHEWERRNTPSS"
     gene            complement(4284..5312)
                     /gene="add"
                     /locus_tag="AC16CB_12705"
     CDS             complement(4284..5312)
                     /gene="add"
                     /locus_tag="AC16CB_12705"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HIR3343374.1"
                     /translation="MRPFEFYQSLPKVDLHRHLEGSLRFETVRDIARSHNLDLPGASQ
                     LRSLVQIQKDEPLTFENFLSKFATLRLLYRSPEIITRITREAIADAAADNVRYLELRF
                     TPVALSKSQGFSLAQVIDWVIEGTRQGEAESGLTTRLIASVNRHEGPTLAAQVADLAV
                     ERRDAGIVGLDLAGSEPGYPAEPFASIFQHAKESGLRIAIHAGEWDHGDNVAEAIEKL
                     DADRIGHGIRVLESPTAIELAQKNQTPFEVCITSNYQSGAVTDLNSHPVAQMLAKGLN
                     VTINTDDPSISQIDLSHEYHLFCETLNFPLEVLRARVLAAAQASFLPESERSKLVQSI
                     DQEFQAHL"
     gene            complement(5391..>7796)
                     /locus_tag="AC16CB_12710"
     CDS             complement(5391..>7796)
                     /locus_tag="AC16CB_12710"
                     /inference="COORDINATES: protein motif:HMM:NF044398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR3343375.1"
                     /translation="PELQYLYEPLEQLDIFSMRKMARELEIHFNPEDTSNIPLLLELL
                     KLESSDGARLSAALQGGIPHQVLNARKHTEESQIIAGAGAFGAVTIATNMAGRGVDIK
                     LGGELSEEAVFAASRVLRRAGYDDPYDMSLEERRQAMLSLNSEDYGIYETEVQQFFKY
                     MEDMQRVRDLGGLHVIGSERHEARRIDNQLRGRSSRQGDPGSSRFYLSMEDDLMRLFG
                     GQQADNLMQRMKIDDDVPLEMGLVSRIVEQSQSRVEGANFDVRKHLLEYDDVLNTQRA
                     KIYAQRNRIFLKDDLSEDVTEMLRTEINQRIPEALQDPEGPWKLLAWMEQIQPPLQFG
                     SQFYPSYMLRLILEHILDGQSGGLSPQAAIQALLEIARQAIQAEEDHLVNAVDMLLEQ
                     ARERLESQIAERDETLDIFFEGLSLADETEQRSLRQIADELSTVVRLPIRLPSEQLRL
                     LRDDPQEAKDLVYEQLMGYLSAQAVTRLVGSVERRLEESLNIDMNQLPAENWDDLSDQ
                     VLSAIQLAFEGRRERYLGEGGQIARELENAIGRADGKLNYNALLQLLMLMPQGARAAF
                     DKKTHRRVYHRTTRFSYVYHAASYLEGREPEAIADDVLKHLELAQAAIRQAWGINEWN
                     RIAASSIQDLDTTTQKGLRSVLSEEDYLSVHQRPLQGLPGEKVERVVNELGRQALTEI
                     YRQLLLGIITELWVDYLTQMEALRVSIGLEAYAQRDPLVQYKNRAFGLYQDLLGNMRS
                     GVVGRMFTYRPRDLSAVQTTVSRVEAPEVEAVVSGNGPQSAPQIEKETRGGKPKKRRR
                     RRR"
BASE COUNT         1611 a         2153 c         2234 g         1798 t
ORIGIN      
        1 tggtaggtgt gtatggcccc ggtacgataa ccgatgagat catcaaggat gttcgccagg
       61 cggtggctgg ggcggcggtg gcttaagttc tcatgggagt ggttgactct gtattggaag
      121 gcaaccggct ctcgttagcc aggttgctca cccagattga gaacggcgtg cccgaaggtc
      181 gcgctgccat ggaggcgctc taccctcata ccggtaaagc tcacctgatt ggtgtcactg
      241 gtgcgcccgg gacaggtaaa tcgtcactgg ttaaccagct ggcgcgcttg tttcgctacc
      301 cgcctagtga ggattcaccg cagcgcgttg gggtggtggc tgtcgacccc tccagcccgt
      361 ttacaggcgg ggcgatcctg ggtgaccggg tacgcatgcg cgatctggcg ggcgatccgg
      421 gtgtgtttat ccgctcgatg gcctcgcggg gcacgctggg cggcttggca acagccaccg
      481 ccagcatggt actggctctc gacgcggcgg gcttcgaagt aatcctaatt gagacggtcg
      541 gcgcgggtca ggcagaggtg gatattgccc gcctggcgca caccacacta gttgtcgaag
      601 cccctggtct gggtgatgat attcaggcta ttaaggctgg catccttgag atcgctgata
      661 tcctggtggt gaacaaagcc gaccgacccg gcgtagagaa caccgagcgt gccctgcgag
      721 gtatgctgca attggggcat cctgctccgc gtgtatactt gcatcacggt cagacggaaa
      781 ggttaactcc gcctgcagac ctggaagctg acaacttatt gtggcagact cccgttgtgc
      841 gcaccgtcgc caccgagggc accggcgtgg atggtttata tcaggagatt gcccgccacc
      901 ggcgctacct gcatgagagc ggcgagtggg agcgccgtga gcgcgcccgc ctgcaatctg
      961 aactggatat actgctccag gcggctctgg tctcgcgctg gagagagacg gtctcccaag
     1021 aggcgtatcg aggcaccgtg gacaggttgg tgcgcagaga catttccccc tggcaggcgg
     1081 tggagagctt gctcgacggt cacggataat aaccagcatc ctactgagga gagagcaatg
     1141 atctacggcg aacgcatccg actgcgcgct ccagagcgag atgacttgcc tcgctttgtt
     1201 ggttggctga acgacccgga ggtacgccag aaccttttca tgttcttgcc actctcgatg
     1261 gcagaggaag agcactggtt cgaggatgtg ttgaagcagc cagcagcaga gcgcccgatg
     1321 gtgatcgaaa tcctggacgg ggagagctgg aagccggtag gtaactgtgg ctttatccac
     1381 atcgattggc gctgccgggc cggcgaggtg ggcatcttca tcggagataa atcacgatgg
     1441 aaccagggct atggcacaga agcgatgcgt ctgctcttgc ggcatggttt taacaccctc
     1501 aacctaaacc gcatatttct tcgtgtattc gataccaatg cccgcgccat gcgcacgtac
     1561 gaaaaggcgg gctttgtcca tgaggggcgc cttcggcagg cagaatacca ggatgggaag
     1621 tacatcgata tgctggtcat gagcgtgcta tactcagaat ggaaagaaaa ttaggagcag
     1681 gggttcttat gcttactcac tatcttcatc gagataccaa tatgtacgga gacatcaagc
     1741 tgtttgcagg tactgcctgc cctgagttag ctggaacgat tgccaagtat ctcgaattgc
     1801 ctctcagcgg gcgggatgtc actttattcc ccaacgacaa tctctttatc cggctgcaca
     1861 aaagcgttcg aggtcaggat gtgtatgtga tccagaccac ctcccggcct gttcaccgta
     1921 atttgatgga gctgctcatc atgctccaga cgctgcgcct ggactctgcc ggtcgagtga
     1981 cagcggtcgt cccatacatg tgctatgccc gctccgataa gaaagataaa ccgcgcgtgc
     2041 cgatcactgc acgtctggtc gccgatatga tcgagatcgc cggagcagac cgctatatga
     2101 cgctcgatct gcatgctgga cagatccagg gctttttctc catccccggt gatgtgttga
     2161 ccgctttcca tattctcact gctcatgtcg agaagatgct tccagcactc aaggacccag
     2221 tagtggttgc agcggatctg gggtttgcca agaagggacg caacttcgct gccactattc
     2281 aggcgccgat tgccttcatt gagaagcggc gcagtgctaa ggaagaccag ccaatcgccc
     2341 tgaccatcat cggtgacgtt aaagatcgag atgtgatcat catcgacgat gaggtagata
     2401 ctgctagatc ggtcgagcag gcggtcaatc tggtcaaagc tcatggggcg aggaatgtct
     2461 acttgatttt tgtccatgcg gtgctctccc cgccagccat gcagcgcatt gcccaactgc
     2521 ctttaaagga gatcatcacc accgatacca tccctattcc ctcggatcag agagcactga
     2581 tccaagatcg cttgacaatt ctctctgtgg cgcccctgtt gggggaggtc atcctgcgcg
     2641 cccatgaagg gcgtagtgtg ggcgagctat ttgacgagtg agacatgccc gaattgcccg
     2701 aagttgagac gatcgccgcg aatttgcggg agggtaaccc aaaccaacca tccttaatcg
     2761 ggaagagcat acaacaggca cacttgctgt gggagcgcac ccttgctacc ccttcggcaa
     2821 gcgaattcta ttcctggata cctggacaat ctatccttga tattggccgc cgaggcaaat
     2881 ttatcgtctt tgagctgagc cgccacaccc tgctgatcca cctgcggatg agtggcgacc
     2941 tgctggtgga accagtcgat gctgttatag cgccccatca ccggctcgtg attgatctcg
     3001 atggcggtat ccgcctagct ttcaacgatg cacgtaagtt tgggcgcgtc tggctgacag
     3061 agaaccccag cggcgtattg gctgctctgg gtccagaacc attggatgaa tctttccgcc
     3121 cacttgattt gcaccagcgg ttgcaggctc atcgccgcca attaaagcct ttgttgcttg
     3181 accaaagctt cctggctggt ttgggcaata tctacgccga tgaagccctg catttagctg
     3241 gcattcatcc attaacccag tcaaacatgc tttccctgga gaaaagcgag cggctttggc
     3301 agggcatccg tgaggtgctg caggagggca tcctgcgcaa tggcgccagc attgactggg
     3361 tgtaccgggg aggggacttt cagaattact tccgggtgta ccagcgcacg ggaaatccct
     3421 gttacaattg cggtactccc attgaacgct tggttgtggg gcaacgcggc acacattttt
     3481 gccccaattg tcagcctttt gacggcagga ggcattcatg aacgcgaaca ttatgctgat
     3541 tgttgttggc ctgatcggaa tagcgatcgg ctttgctctg ggcgtggcct tgatgagttt
     3601 gcgtagccca caagccgagg aaaacgagaa agaacctcct ccgctccaga agcgtatcca
     3661 aacgcctccg ccaaaggctc ccccaacagt cgatgttgcg cctcgccccg tctcaaaacc
     3721 cactccttcg ttggaagact ctcctcctcc acagcaacgc cccaccataa agccagccaa
     3781 tgttctggcg cgtgtcctgc agcccgactt aagcgctgcg caagcgcccc ctaaaagcat
     3841 tgccgcccag atcgacgaga tcctgcagga gatgctggag gattctcccc tggactcgag
     3901 gggaatccgc ttgatggagt ttcccaatcg agggctggtg gtcatggtcg ggctggacca
     3961 gtacgagggt gtggatgcag ttcctgatga ggatatccgc agactaatcc gaagcgctgt
     4021 tcatgaatgg gagcggcgta acaccccctc ttcttagaaa tcataacccc agccaaaaag
     4081 cggctcgcac tttgtggtct acgcaaaaag atagcacgtc attgagagcc ccaaaggggc
     4141 gaagcaatct cagctttgcc tggagattgc cacgccgccc tccatccggc ttgcaatgac
     4201 gctacaatca aattgggagc atatgggtgg gcgaaatcca gccataggct cctaattgtt
     4261 tcctcaactg cgcgtttctg attttatagg tgggcttgga attcttggtc tattgactgg
     4321 acgagcttgc tgcgctcgga ttcgggcaag aaagatgcct gcgccgcagc tagcacacgc
     4381 gctcgcagga cttcgagggg gaaattcagg gtttcacaga ataaatgata ttcgtggctg
     4441 aggtcgatct gtgagatgct tgggtcatcg gtgttgatgg tcacatttaa ccctttggca
     4501 agcatctgag ccaccgggtg ggagttcaag tcggtcaccg cgccggactg atagttgctg
     4561 gtgatgcata cttcgaaggg ggtctggttt ttttgagcca gttctatcgc tgtaggtgac
     4621 tcgagcacgc gtattccgtg accgatgcga tctgcgtcca atttctcgat agcttcggct
     4681 acattgtcgc catgatccca ctcgccagca tgaatcgcaa tgcgcagtcc agactctttg
     4741 gcatgctgaa aaatcgaggc gaaaggttca gctgggtatc cgggctcaga gcctgccagg
     4801 tccagcccca caatgcctgc atctcgcctc tccactgcca ggtcggctac ctgtgcagcc
     4861 agggtgggac cctcatgacg gttaacgctg gctatcaagc gagtggttaa cccgctttct
     4921 gcctcacctt ggcgggtgcc ttcaatgacc caatcgatga cttgtgccag cgagaaaccc
     4981 tgagatttgc tcagcgccac cggtgtgaaa cgcagctcta gatagcgcac gttgtccgcg
     5041 gcagcatccg cgatagcttc cctggtgatg cgggtgataa tctcgggtga gcggtagagc
     5101 aggcgcaggg tcgcgaattt ggataagaag ttctcgaagg taagcggctc atccttttgg
     5161 atctgcacca agctgcgcaa ttgactggcg cctggcaaat ccaggttatg ggagcgggcg
     5221 atatcacgca cagtctcgaa gcgtaatgag ccttccaggt ggcggtgcag gtccactttg
     5281 ggcagcgatt ggtagaattc gaatggtcgc atccgtggct cggcgaggtt catgtagttt
     5341 aagtgcttcc catatctttc gcagtgaagc cttcaaagaa ctattcggtt ttaacgccgc
     5401 ctcctgcggc gtttctttgg ttttccaccc cgcgtttctt tttctatctg tggagccgat
     5461 tggggtccgt tcccggaaac cacggcttct acctcgggtg cttcgacacg gctgacggtg
     5521 gtttgcacag cactcaggtc gcgcggccgg taggtgaaca tgcggcctac cactccagag
     5581 cgcatattac cgagcaaatc ctggtacagg ccgaaagcgc ggtttttgta ttgcaccagc
     5641 gggtcgcgct gtgcatacgc ctccagcccg atggagacgc gcagggcttc catctgtgtc
     5701 aggtagtcca cccacagctc agtgatgata cccaatagaa gttgccggta gatctcggtg
     5761 agcgcttggc gaccaagctc gttaacgaca cgctccacct tctcacctgg caagccctgc
     5821 aatggtcgtt ggtggacgct aagataatcc tcctcgctca gcacactgcg caatcccttt
     5881 tgggttgtag tgtctaggtc ttgaatggaa gatgcagcga tgcgattcca ttcattgatg
     5941 ccccatgctt gccggatggc ggcttgggca agctctagat gcttgagcac atcgtctgca
     6001 atggcctctg gctcgcggcc ttccagatag cttgcggcat gatagacata gctaaatcga
     6061 gtggtgcggt ggtagacgcg ccggtgagtt ttcttatcga aggcagcccg cgccccttgc
     6121 ggcatgagca taagcaattg gagcaaggca ttgtaattca atttcccgtc cgctcgaccg
     6181 atggcatttt ccagctctcg agcgatctgg cctccctcgc cgaggtaacg ctcgcgccgt
     6241 ccttcaaagg caagctggat ggcagataat acctggtcgc taagatcgtc ccaattttcg
     6301 gcaggcagct ggttcatatc gatgttgagc gactcttcca aacggcgttc tacgctaccg
     6361 accagcctag tgaccgcctg cgcagacaga tatcccatga gctgctcgta gaccaggtcc
     6421 ttggcctctt gcgggtcgtc tcgcagcagg cggagttgct ctgatggcag acggataggc
     6481 aggcgcacta cagtggagag ctcgtccgcg atctggcgta ggctgcgctg ctcggtctcg
     6541 tctgccaggg ataagccttc aaaaaatata tctagggttt catccctctc ggcgatttgg
     6601 ctttccaggc gctcccgcgc ctgctccaat agcatgtcga cggcgttgac tagatgatct
     6661 tcctcagcct ggatggcttg tctggcgatc tctagcagag cctggatggc agcttgtgga
     6721 ctcaatccac ccgattggcc atccaaaata tgctcgagga tcaagcgcaa catgtaagat
     6781 ggatagaatt gtgagccaaa ctgaagcggg ggctggattt gctccatcca ggccagtagc
     6841 ttccagggac cttctggatc ctgtaaagcc tcagggatgc gctggttgat ctcggtgcgc
     6901 aacatttcgg ttacatcttc gctgagatca tctttaagga agatgcggtt acgctgggcg
     6961 taaatcttgg cgcgctgggt gttgagcaca tcgtcgtatt ccagcagatg tttgcggacg
     7021 tcgaagtttg ccccctccac gcgggattgg gattgctcca cgatgcggct gaccaggccc
     7081 atctccaggg gcacatcatc atcgatcttc atgcgctgca tcaggttgtc ggcttgttgt
     7141 ccgccaaaca agcgcatcaa atcatcttcc atggagaggt aaaaacgtga cgagccgggg
     7201 tcgccctggc gtgaagaccg gccgcgcagc tggttgtcga tccggcgggc ttcgtgacgc
     7261 tcagagccga tcacgtgcag gccgcccagg tcgcgcacgc gctgcatgtc ttccatgtat
     7321 ttgaagaact gctggacttc agtctcgtaa atgccgtagt cttcgctgtt gaggctcaac
     7381 atggcctggc ggcgttcctc caggctcatg tcgtatggat catcgtaccc ggcgcggcgc
     7441 aacacgcgac tagcggcgaa gactgcctcc tcggacagtt ctcctcccag cttgatatct
     7501 acaccgcgtc cagccatatt tgtagcgata gtcaccgccc caaaagctcc cgccccggcg
     7561 atgatctggc tctcttcagt atgcttgcgg gcgttgagca cttggtgggg tataccccct
     7621 tgcagtgctg cgctcaggcg agccccatca ctgctctcta atttaagtaa ctccagcaag
     7681 agagggatat tcgaagtatc ctccgggttg aagtggattt ccagctcacg cgccatcttg
     7741 cgcatgctga atatatccaa ttgttccagc ggctcgtaca ggtattgcag ctctgg
//
LOCUS       DBNGSX010000428         2448 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1010011, whole genome shotgun sequence.
ACCESSION   DBNGSX010000428 DBNGSX010000000
VERSION     DBNGSX010000428.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1010011"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="AC16CB_12715"
     CDS             complement(<1..244)
                     /locus_tag="AC16CB_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343376.1"
                     /translation="MKNKLVNSQLLSEPRLRSWLVPHKITLLIVWLVTTISMIVILID
                     GVGGTNLSIAIRQMLHVLYVVALLWYLVRIGPSLNQL"
     gene            complement(259..906)
                     /locus_tag="AC16CB_12720"
     CDS             complement(259..906)
                     /locus_tag="AC16CB_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343377.1"
                     /translation="MSENKRKPSGWYYLLALLIPVFACLGTVLLVYPNVPELPGALDE
                     IGVKNLTQVIVPGSADVSFPKKGAYAVYYEYRSVIDGVSYARNEYSPIMRWQLRSIDT
                     SEVVRLAPTNVKGNVYTTRNPHRAAVMYKQISIDQPGIYTFSCQYTNGETIPKSVMAV
                     GPNIVWGFFNIAAKPIAAIFFGTIAFFLACWTSILIIGFVAFKRHQSKNILDSKA"
     gene            complement(899..1957)
                     /locus_tag="AC16CB_12725"
     CDS             complement(899..1957)
                     /locus_tag="AC16CB_12725"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR3343378.1"
                     /translation="MNKVTNWIKSHQVAAFFILVYVIAWPGMFLVYFVFPGNVVVEAL
                     SMPFVLFSPALTAMLISGIAEPRPKHEDSKPRWIAFILSWIISGPIIILHAKNNMGIE
                     SVPAFIVFGIMGIFPAWVLSSAYARTPGIRKLFSTLLKPRRPAIWYLVVFLIFPGFIL
                     LAFYITSILGGKAQFYLADLGIGGAAVFLFLEFSKGFVLTGGINEESGWRGFALPRLQ
                     ARYPVIVAGLIVGYLWAFWHLPYDIGEGVPTSWLLQNRLFWTPLMGIIMTWLYNRTNG
                     SLLAPALFHPAMNAFGNHFSITPVGNILFIGLTIYAIVSDRMWQKLPPDYPAVYQELN
                     SFHLEKENKKEILEVSRV"
     gene            complement(1954..2235)
                     /locus_tag="AC16CB_12730"
     CDS             complement(1954..2235)
                     /locus_tag="AC16CB_12730"
                     /inference="COORDINATES: protein motif:HMM:NF025601.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HIR3343379.1"
                     /translation="MDTTQNTNANDKSNWTSIGSAVREILRESRNRRVVIRKASGKKV
                     IDCSVLLALILSIGAPVLPAFVILGVLVESISVSFEKRGVQISTKETIQ"
BASE COUNT          694 a          575 c          573 g          606 t
ORIGIN      
        1 gtagttggtt gagggaaggt cctattcgaa ccagatacca taacaacgca accacataga
       61 ggacatgcag catttgacgg attgcgatgg agaggttcgt gcctccgaca ccgtctatga
      121 ggatcacgat cattgagatg gttgtcacca accagacgat cagcaaggta attttatgtg
      181 gcaccaacca ggatcgtaac cgaggttctg agagcaattg tgaatttact agtttgtttt
      241 tcatctttca cctctccgct aggctttcga atccaagatg ttcttggatt ggtgtcgctt
      301 gaaagccacg aaaccgatga tcagtatgga tgtccagcag gcaaggaaaa aagcgattgt
      361 tccaaaaaag atagcagcga tgggctttgc tgcgatattg aagaatcccc agacaatatt
      421 tggaccaaca gccattacgg atttcggaat cgtttcgccg tttgtgtatt gacaagagaa
      481 tgtgtagatg cctggttgat caatgctgat ctgtttgtac atcacagctg cgcgatgtgg
      541 attccgggtg gtataaacat tccctttaac attggtgggg gcgagtctga cgacttcact
      601 ggtatctatt gacctcagct gccatctcat aatgggagag tattcgtttc tggcgtaact
      661 caccccgtcg atgacgctgc ggtattcata gtaaacggcg taagcgccct ttttggggaa
      721 agaaacatcc gcagaacccg gaacgataac ctgggtcaga tttttcactc caatctcatc
      781 cagtgcgccg ggcaattcag gtacgttcgg atacaccagc agcaccgttc ccagacacgc
      841 aaaaactgga attaataaag ccagtaagta ataccagcca gagggttttc gtttattttc
      901 agacacgtga gacctccaag atttcttttt tgttttcttt ctccaaatga aaggagttca
      961 attcttgata cactgccggg taatcggggg gcagtttctg ccacatccga tcagacacaa
     1021 tagcatagat tgtcaagcca atgaataaaa tatttcctac aggggtgatc gagaaatgat
     1081 ttccaaatgc attcatagca ggatggaaca gggccggcgc cagcaagctc ccattggtgc
     1141 ggttatagag ccaggtcatg ataatgccca tcaatggggt ccaaaacaag cgattttgga
     1201 gtaaccatga tgtaggcaca ccctcgccaa tatcataggg cagatgccag aaagcccaga
     1261 ggtatcctac gatcaaacct gctacgatca cggggtatcg ggcctgcaaa cggggtagag
     1321 caaagcctcg ccaaccactc tcctcattga tgccgccagt aagcacgaaa ccttttgaga
     1381 attcaaggaa caaaaacaca gcggcccccc caattcccag gtcagccagg tagaactggg
     1441 ctttaccgcc cagtatggat gtgatataga atgccagcag aatgaaaccg gggaatatga
     1501 gaaagacaac caggtaccat atcgccggtc ttcttggctt caataaggtc gagaatagtt
     1561 ttcggatccc aggcgtcctg gcataagcgc tggaaagcac ccaggcaggg aagattccca
     1621 tgatcccgaa cacgataaat gccgggaccg attcaattcc catattgttc tttgcgtgca
     1681 ggatgataat tggccccgaa atgatccatg ataagatgaa agcgatccaa cgcggcttgc
     1741 tatcttcatg cttggggcgt ggttcggcaa tcccagagat caacatggct gttagggcag
     1801 ggctgaaaag gacaaagggc atggataggg cttccacgac cacatttccc gggaaaacga
     1861 agtagaccag gaacatgccc ggccaagcta tcacataaac caagataaag aaagctgcaa
     1921 cttgatgact cttgatccag ttcgttactt tattcattgg attgtctcct ttgttgatat
     1981 ttgtactccc ctcttttcga acgaaacact aatcgactca accaacacgc ctaggatcac
     2041 aaaggcggga agaacaggtg caccgatgga taaaatcaat gccagtaaca ccgaacaatc
     2101 gatgactttc ttcccagaag ctttcctgat cacgacgcgc cgattgcgac tctcccgtag
     2161 gatttcacga acagcactac cgattgaagt ccaattggat ttgtcatttg catttgtgtt
     2221 ttgggtggta tccatgataa tttctcctga tcttttgttt gcctttttct tgtgcttagt
     2281 acaacacact tataaacgca catacagaat agcatgcagg ggaaagaatc acacgaccca
     2341 tagtgtatgt tttgccccag cctacggaaa ggttcttaat ccaagctcaa gattgaatat
     2401 ttaattactg cggtactgga aaagccctat taaattaccc ccagagaa
//
LOCUS       DBNGSX010000429         2732 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1078939, whole genome shotgun sequence.
ACCESSION   DBNGSX010000429 DBNGSX010000000
VERSION     DBNGSX010000429.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1078939"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="AC16CB_12735"
     CDS             complement(<1..251)
                     /locus_tag="AC16CB_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3343380.1"
                     /translation="MMEYRYLGRTGIKVSELCLGSMQFGWTASEEDSYQILSAAFEAG
                     INFIDTTDVYSRWVEGNPGGVAEAIIGKWMKRNNIPRHQV"
     gene            complement(310..849)
                     /locus_tag="AC16CB_12740"
     CDS             complement(310..849)
                     /locus_tag="AC16CB_12740"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3343381.1"
                     /translation="MSTYTVIDSQTLFEHPGVKIVVDTLDHLGKQRRYFYLASPVNAV
                     ATVGLTNDRQLLLTRQYRHPVGQVIYDLPAGRLNAGESPLEGARREFEEETGYYPNHI
                     EQLGFYNQFPGTLRAATTLFFARDLVPTHQHLDEGEELDIVIKPVPEVLEMILGNEII
                     DGSLQLGVLLALQKGLIKS"
     gene            complement(855..1157)
                     /locus_tag="AC16CB_12745"
     CDS             complement(855..1157)
                     /locus_tag="AC16CB_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HIR3343382.1"
                     /translation="MSKLIALFKQPADPTAFDQAYFNTHLPLIAKVPGLNKTVVTRFT
                     RTLQGEGFYLMAEMVFDDREALKTGMKSPEMAAAGANLNTFAAGLVTLMFAEEESR"
     gene            complement(1182..1658)
                     /gene="paaD"
                     /locus_tag="AC16CB_12750"
     CDS             complement(1182..1658)
                     /gene="paaD"
                     /locus_tag="AC16CB_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008880601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HIR3343383.1"
                     /translation="MTPEEAWRILDEIKDPEIPVVSIVEMGMLRHVEIEDQTVTVTIA
                     PTFVGCPAIQVIQEHVTRSLEEAGAETVNVRFTYSPPWSSDAITPEARKKLKDFGLSP
                     PQMHQGLIEIALLDPAACPYCDSTNTSMRNSFGPTLCRSIHYCNNCQQPFEAFKPL"
     gene            complement(1668..2420)
                     /gene="paaC"
                     /locus_tag="AC16CB_12755"
     CDS             complement(1668..2420)
                     /gene="paaC"
                     /locus_tag="AC16CB_12755"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015234705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HIR3343384.1"
                     /translation="MKTALREYLLALADDELILGHRASEWCGHAPILEEDIAFANLAL
                     DEIGHASLWYSVLAEIEEQDPAVYPDRLAYFREPQDFRNIQMVELPKGDWAFTIMRQY
                     LFDAYEQVRLESLLQSSYEHFAQVARKIRQEEFYHYRHSSAWVRRLGAGTQESQRRMQ
                     IALDELWAYTGQLFQPIAGESELIQAGYVIASENLKDKWQDKVYPTLIESSLVIPATP
                     RLQLNRQTHTHHLEVLLSEMQSVARLEAEANW"
     gene            complement(2417..>2732)
                     /locus_tag="AC16CB_12760"
     CDS             complement(2417..>2732)
                     /locus_tag="AC16CB_12760"
                     /inference="COORDINATES: protein motif:HMM:NF018002.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343385.1"
                     /translation="SVLEPAPSEKYHVFCKFKHAGTQIQVGRAEASGPAQALEKAIEK
                     YSSHHKPLVWWVFPARMVTQSDPSETGSLYEPAQDKMFRMHTQFHTLTAMRDIEQSKG
                     RK"
BASE COUNT          628 a          681 c          707 g          716 t
ORIGIN      
        1 acctggtgac ggggaatatt attgcgcttc atccatttac cgatgatggc ctctgccacc
       61 cctcctggat ttccttccac ccagcgagaa tacacatcgg tggtatcaat aaaattgatc
      121 ccagcctcaa atgctgcgct aaggatttgg taggaatctt cctcactcgc agtccaacca
      181 aattgcatag accctaagca gagttcagat acttttatac ccgtgcgacc caggtaacga
      241 tattccatca tgctccttta tttagtagat ctcacttcag tgaacaaaag ctgcctggaa
      301 aacagatcgt taagatttaa tcagcccttt ttgaagggca agtaataccc cgagttggag
      361 ggagccatcg atgatctcgt tacccaagat catctccaat acttcgggca caggcttgat
      421 cacaatgtcg agctcctcac cttcatccag gtgttggtgg gtagggacga ggtcgcgggc
      481 gaagaacaag gtcgtcgcgg ctcgcaatgt gccaggaaat tggttgtaaa agccaagctg
      541 ctcaatatgg ttgggataat agccagtttc ctcttcaaac tcgcgccggg cgccctccag
      601 tggcgactca cctgcgttga gtcgtccagc cgggaggtcg taaattacct gtccaactgg
      661 atggcggtac tggcgcgtta ggagaagctg tctatcattg gtcagtccta ccgttgcaac
      721 agcgtttact ggactggcaa ggtaaaagta gcggcgctgt ttcccaaggt gatccagtgt
      781 atcgacaaca attttgacac ccggatgttc gaataaggtc tggctgtcga tcacagtgta
      841 ggtggacatg ggtgctagcg gctttcttct tctgcgaaca tcaaggtgac cagccctgcg
      901 gcaaatgtat tcaggtttgc tcctgctgca gccatctctg gacttttcat gcctgtttta
      961 agcgcctccc ggtcgtcgaa gaccatttcg gccattaaat aaaagccttc gccttgtaga
     1021 gttcgggtga agcgagtgac aacggtcttg tttaaaccgg gtacttttgc tatcaggggc
     1081 agatgggtat tgaagtaagc ctggtcaaat gctgtgggat cagcgggttg tttaaatagg
     1141 gcaatcaatt tggacattct ggtaatctcc tgtcgaagct ttcagagagg tttaaaggct
     1201 tcaaacggct gctggcagtt gttgcaatag tggattgacc ggcaaagggt agggccgaaa
     1261 ctattgcgca tggatgtatt ggttgaatcg caatatgggc aagctgcagg gtccagcagt
     1321 gctatctcga tcaatccctg atgcatttgt ggaggggata ggccgaaatc cttcaatttt
     1381 tttcgagctt cgggtgtgat tgcatcactg ctccaaggtg gtgaataggt aaagcgaaca
     1441 ttgacggttt ctgcacccgc ctcttctagc gagcgggtta catgctcctg tatgacctga
     1501 atagccgggc aacctacgaa ggtgggcgca atagtgacgg tcactgtctg gtcttcaatc
     1561 tctacatggc gcagcatccc catctcaaca atagagacca cgggaatctc gggatctttg
     1621 atttcatcta atatgcgcca ggcttcttca ggggtcattt tgggctctca ccaatttgcc
     1681 tcagcttcca agcgggccac cgactgcatc tcgctcaata gcacctccag gtggtgtgtg
     1741 tgcgtttggc gattcaattg taatcgcggg gtagcaggta tgaccagtga agattctatc
     1801 agtgttggat agaccttatc ctgccacttg tcttttagat tttctgatgc gatgacatac
     1861 ccggcttgga ttaattcgct ttccccggcg atgggctgga atagctgccc ggtatacgcc
     1921 cataactcgt ccagtgcgat ttgcatgcgt cgctggcttt cttgggtgcc tgcccccaaa
     1981 cgacgcaccc aggcagatga atggcgatag tgataaaact cttcctggcg gattttcctg
     2041 gcgacttgcg caaaatgctc atagctactt tgtaataagg attccaaacg cacttgttcg
     2101 tacgcatcaa agaggtactg tcgcatgatt gtgaaagccc aatcaccttt gggtagctcg
     2161 accatctgga tatttctgaa atcctgtggc tcgcgaaaat atgccaagcg gtcgggatat
     2221 acagccgggt cttgctcttc gatctcagct aacacgctgt accacaggct ggcatggccg
     2281 atctcatcca aggccaggtt ggcaaaagca atatcctctt ccaggattgg cgcgtgaccg
     2341 caccattccg aggcacgatg ccccaggatt agctcatcgt cagcaagagc cagcaaatat
     2401 tctcgaaggg cggttttcat tttctgccct tcgattgctc gatatcacgc atggcggtca
     2461 aggtatggaa ctgggtgtgc atgcgaaaca ttttgtcctg cgcaggctcg tacaagctgc
     2521 cggtctccga gggatcgctt tgggtaacca tgcgggccgg gaatacccac cacaccagag
     2581 gcttatgatg gctcgagtat ttctcaatgg ctttctccaa agcctgtgcc gggccagaag
     2641 cctccgcgcg accaacctgg atctgtgtcc ctgcgtgttt aaatttgcaa aagacgtggt
     2701 atttttcact cggagcaggt tctaaaacac tg
//
LOCUS       DBNGSX010000430         6255 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_247527, whole genome shotgun sequence.
ACCESSION   DBNGSX010000430 DBNGSX010000000
VERSION     DBNGSX010000430.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6255)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_247527"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="AC16CB_12765"
     CDS             <1..559
                     /locus_tag="AC16CB_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HIR3343386.1"
                     /translation="VLKTAFNPSLRNVPWRDAPQDLQAWKAGLTGYPVVDAGMRQLAG
                     TGWMHNRARMIVASFLVKDLLVNWQDGERWFMQSLVDGDPAANNGGWQWTAGVGTDAA
                     PYFRIFNPVLQGIKFDPSGSYVRRWVPELVKVPEKWIHRPWEMPQEAQLAVGFKPGKD
                     YPLPIVDHALARQRVLEAYKRAAKG"
     gene            complement(622..1311)
                     /locus_tag="AC16CB_12770"
     CDS             complement(622..1311)
                     /locus_tag="AC16CB_12770"
                     /inference="COORDINATES: protein motif:HMM:NF025636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3343387.1"
                     /translation="MSTTNSAERSLLNNFRKSSKSGSKERKVDRRVYRTRRALREAFF
                     ALILEKGYEAVTIEEITNRADLGRTTFYLHYRDKEDLLMESIGELVDDLVERMSHLPL
                     GDWKIDLNFSNSQAPPAITLPFQHVAQNAQLYRIILRGEGNFSITARLREIIVQASGE
                     IIQVMLKKEKLAINPQLPLEVFMNYLAASWLGLITWWLEKEMPYTPEQMATMFQMMFI
                     RGARDVLGLTS"
     gene            1379..2161
                     /locus_tag="AC16CB_12775"
     CDS             1379..2161
                     /locus_tag="AC16CB_12775"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3343388.1"
                     /translation="MREKISKNGNGRTSIANPPHPLNGNGRTPLVRLRGVSKSYQTAA
                     GDFLALSGIDLDVYPGEFLGIIGKSGAGKTTLINILTGVDRLTSGEVWMDELPIHRLD
                     ENQLATWRGRNVGIIYQSFHLMPNLSLLQNVILPMDVSGLYRSGESEQRALELLQMVE
                     LEDHARKPPSAISGGQQQRVAIARALANDPPIIVADEPTGRLDSSTAETIFQIFTRLM
                     EEGKTILMVTHDQSLARRMTRTMHLVNGGIAHQHGMQLRGRG"
     gene            2179..3336
                     /locus_tag="AC16CB_12780"
     CDS             2179..3336
                     /locus_tag="AC16CB_12780"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3343389.1"
                     /translation="MKGTNQPNYLPLPNRCSDDALIEMAQIVKTFKNAAGEFTALNEV
                     TACFYEGEFVSVVGKSGSGKSTLVNMITGIDHPTSGTVRIGDAYVHALSESDMSIWRG
                     KNLGIVFQFFQLLPTMSLLENVMLPMYLSGQYSPAEREERALSLLKLVGLEGLDDKLP
                     AAVSGGQQQCAAIARALANDPPILIADEPTGNLDSRTAEEIYDLFAGLIDQGKTILMV
                     THDISLSERTTRTLLISDGELINPWVASALPHLGHSQLLWLTHNLKARQLPAGAIVPD
                     LHSQGDWLILVAGGQLEVLDDRGGHREQPLMRLGAGNALSSLDLQASGIKQPRLRAAG
                     EAVEFLVTEGQELKRWLNETPTNRSILQKVEQVSPLMMAGRVRDGYRGLSV"
     gene            3333..5747
                     /locus_tag="AC16CB_12785"
     CDS             3333..5747
                     /locus_tag="AC16CB_12785"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR3343390.1"
                     /translation="MKRPRFQKVLADLWGNRTRSLLVIASIGVGLFALGVMLTIYVVT
                     LDDMQRGYAAIDPANIFVQTTLFDDGLVKGIEEVEGVRLAEGVRIFGTRLKASPEQWI
                     SINIQSFKDPGQQQINQVRLEEGVWPPGDKEIVLDRYKLHDTNARLGDYLTIETPAGK
                     SRQLKLVGVIQDQSIGAFRSAGGFFNAPVQGYINQDTLEWLGQPLPDRFDTLYITVAG
                     DSTDTAYLREISASVRDKIEKNNAQVISTSLRSSYEHPNLYLVMAVLAVLVVIGLLVV
                     LLSGFLITNTLQALLNQQVGQIGILKSVGARRVQIVGLYMMLSLFYGLLALAIALPLS
                     FEVAFRIVDFLTIQMNNSFFGFRILPEVILVEAAIAVLMPQIAALVPLWQGTHISVQE
                     ALSGIRQDHPLGRSWLDRSISRLRNFSLLLVMSLRNTFRRKGRLALTLVTLTLGGAVF
                     IATFNVRVSLIMYIDRIIQYFLADVNVTLNQPYRMDEVRGLIMEVPGVKMVEGWAFAR
                     TELVMADGSVGESVSLLAPPADSPLVKPIIVAGRWIQPGDQNAIALNELFRERYPDLQ
                     VGDTIQLQVDGEENDWVVVGFYQMAGKVSGFSAYTSYEYLSELNHQTGKAISYRVVSS
                     WQGLGRAQQEALGRAIEAHLEGAGLDVVDVTTGMSLSETASNGFNVLTAFLLFLALLT
                     AVVGSIGLTGTMSLNVMERTREIGVLRAIGASNRTLMQMVLVEGVLIGFLSWILASLA
                     AFPISVLIAESISQSLFGASSKFGFTPIGFVIWMLAVLVLSVLASVIPARNATRLTIR
                     EVLAYE"
     gene            complement(5848..5918)
                     /locus_tag="AC16CB_12790"
     tRNA            complement(5848..5918)
                     /locus_tag="AC16CB_12790"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5884..5886),aa:Cys,seq:gca)
     gene            complement(6004..6075)
                     /locus_tag="AC16CB_12795"
     tRNA            complement(6004..6075)
                     /locus_tag="AC16CB_12795"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6041..6043),aa:Gly,seq:gcc)
     gene            complement(6103..6175)
                     /locus_tag="AC16CB_12800"
     tRNA            complement(6103..6175)
                     /locus_tag="AC16CB_12800"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6140..6142),aa:Phe,seq:gaa)
BASE COUNT         1413 a         1579 c         1772 g         1491 t
ORIGIN      
        1 cgtattgaag acagccttca acccttcctt gcgcaatgtc ccctggcgag atgcacccca
       61 agacctgcag gcttggaaag ctggtttaac cggctaccct gtcgtggacg cgggtatgcg
      121 gcagctagca ggtaccggct ggatgcacaa ccgggcgcgc atgattgtgg cttcgttcct
      181 ggtgaaggat ttactggtca actggcagga cggcgagcgc tggttcatgc aatcgctggt
      241 cgacggtgac ccagcagcca acaatggcgg ctggcagtgg accgccggtg tgggcaccga
      301 tgccgcaccc tattttcgga ttttcaaccc ggtattacaa ggcatcaagt ttgacccctc
      361 tggttcctat gtccgccgct gggtgcctga attggtgaag gttcctgaga aatggattca
      421 tcgcccctgg gagatgcccc aggaggccca gcttgccgtt ggctttaagc ccggcaaaga
      481 ttacccgctg cccattgtgg atcacgcgct cgctcgccag cgggtactgg aggcatacaa
      541 gcgagccgct aagggttgaa tatttagttt tctgtagaaa cgactacggg gtaagcctaa
      601 aaaagcatca ctgcaagcct gctatgatgt caaccccaat acatctcgag ctccacggat
      661 gaacatcatt tggaacatgg tcgccatctg ctcgggagta tagggcatct ccttctccag
      721 ccaccaagtg atcagcccca accaggaagc tgccagataa ttcatgaaga cctccagtgg
      781 cagttgaggg ttgatcgcca gcttctcttt tttcagcatc acctggatga tttcaccgga
      841 agcttggaca atgatttcgc gcaggcgtgc cgttatggag aaattgcctt cgccgcgcag
      901 gatgatacga tagagctgtg cgttttgggc gacgtgctgg aacggcaacg tgattgctgg
      961 gggcgcttgg ctgttagaaa agttgagatc gattttccaa tcacctaagg ggaggtgaga
     1021 cattcgttca accagatcat cgaccagctc gccaatgctt tccatgagca ggtcttcttt
     1081 gtcgcggtaa tggaggtaaa atgtggtgcg tcctaggtcc gcccggttgg tgatctcctc
     1141 gatggtgacc gcttcgtagc ccttctcaag aatcagcgca aagaatgctt ctcgcagggc
     1201 ccggcgggtt cgatagacgc gccgatctac tttgcgctcc ttggagccag acttcgacga
     1261 tttgcgaaaa ttgttcagta atgaacgctc agcagaattg gtggttgaca agacgcaagc
     1321 cctccatata attactagac gtatgtctat tatactacag cgtgtttatt aatggaagat
     1381 gagagaaaaa atttctaaaa atggaaatgg tcgaacttca attgctaacc caccacatcc
     1441 gctcaatggc aacgggcgca ccccgcttgt tcgcctgcgg ggagttagca agagctatca
     1501 gaccgcggct ggagatttcc tggctttaag cgggatcgat ttggatgttt atccaggtga
     1561 gttcctaggt attatcggca aatcaggggc aggaaaaaca accctaatca acatactgac
     1621 aggcgtagat cggctgacca gcggcgaagt gtggatggat gaattgccta ttcaccgcct
     1681 ggatgaaaac cagctggcca catggagggg gcggaatgta ggcattattt accaatcctt
     1741 ccacttgatg cccaacctat cccttttaca gaacgtgatc ttgcctatgg atgtcagtgg
     1801 actctatcgc tcaggcgaga gcgagcaacg ggcgctggag ttattgcaga tggttgagct
     1861 ggaggaccat gcacgcaaac cgccatcggc tatatctggc gggcagcagc aacgcgtggc
     1921 aattgctcga gcattagcca atgatccgcc aattattgtg gctgacgaac ccactggtcg
     1981 actggattct tccacggcag agacgatttt tcaaatcttc acccgattga tggaagaggg
     2041 caagacaatc ttgatggtga cgcacgacca gagtctagcc cgacgtatga cgcgcaccat
     2101 gcatttggtg aatggtggta ttgcccatca gcatggtatg caacttagag gtagaggtta
     2161 aaggaataag tgaatagcgt gaaaggaacc aaccagccaa actacttgcc gcttcctaac
     2221 cggtgcagcg acgatgccct tattgagatg gcgcagattg tcaagacctt taagaacgcg
     2281 gctggggaat ttactgccct caatgaggta actgcctgct tttatgaggg cgaattcgtc
     2341 agtgtggtag gtaaatcagg cagcggtaag tcgacactag tgaatatgat cacggggatt
     2401 gatcacccaa cctcgggtac ggtgcggatt ggcgatgcat atgtgcacgc cctgagcgaa
     2461 agcgacatgt cgatctggcg gggtaaaaat ctgggaattg tctttcagtt tttccaattg
     2521 ctgccgacta tgtctctatt agaaaacgtc atgctgccca tgtatcttag cggccaatac
     2581 tcgccagccg agcgggagga gcgcgccctg agcttgctca aacttgtcgg gttggaaggg
     2641 ctggatgata aattgccggc ggctgtatca ggcggtcagc aacagtgtgc ggcaattgcc
     2701 cgggctttgg cgaatgaccc acccatcctg atcgctgatg aacctaccgg caacctggac
     2761 tcgcggacag ccgaagagat ctacgacctc ttcgccgggc tgatcgacca gggaaaaacc
     2821 atcctgatgg taacccacga tatcagcctc tcagagcgca cgacacgcac gttgctcatc
     2881 tccgatggcg agctgatcaa cccctgggtg gcgagcgcgc tgccacatct cgggcacagc
     2941 cagttgctat ggctgaccca caatctgaag gcgcgccaac tcccagctgg tgccatcgtc
     3001 ccagatctgc atagccaggg ggattggctg atcttggtcg cgggtgggca gctggaggtt
     3061 ctggatgatc gtggggggca ccgcgaacaa cccctcatga gactaggagc agggaacgct
     3121 ttgagcagcc tagacctgca ggcgtccggt atcaagcagc ccaggttacg agcggctgga
     3181 gaagcggtag agttcttggt tacagaaggg caggagctga agcgctggct gaatgaaaca
     3241 ccgaccaatc ggtcgatctt gcagaaggtg gaacaggtat cccccctgat gatggccggc
     3301 cgagtgcgcg atggttacag aggcctaagc gtatgaaacg ccctcgcttt caaaaagttc
     3361 ttgccgattt gtggggcaac cgcacgcgat cattgttggt aattgcctcc attggtgtag
     3421 gactgtttgc cctaggtgtg atgctgacaa tctacgttgt gacgctggat gatatgcaac
     3481 gcggctatgc agccattgat cctgccaata tcttcgtcca aacgaccctg tttgatgacg
     3541 ggcttgtcaa gggcattgaa gaggttgagg gagtgcgcct tgcagagggt gtgcgcatct
     3601 ttggcacccg attgaaagct agcccggagc aatggatttc gatcaacatc cagtcgttta
     3661 aggatcctgg tcaacaacaa atcaatcagg tgcgtttaga ggaaggggta tggcctccag
     3721 gcgataaaga gattgttctt gaccgttata aattgcatga taccaacgct cgcctgggcg
     3781 actatttaac catcgagacg cccgcgggca aatctcgcca gctaaagctg gttggcgtga
     3841 tacaggatca gagcattgga gccttccgta gcgcaggtgg ttttttcaat gccccagtgc
     3901 aaggttatat caaccaagat acactggagt ggctgggcca acccttacca gaccgcttcg
     3961 atacgttata tattacggta gcaggggaca gcaccgatac ggcttacttg agggagatat
     4021 cggcctccgt gcgggataag atcgagaaaa acaatgccca ggtgatcagc acttcactgc
     4081 gcagctctta tgagcaccct aatttgtatc ttgtcatggc ggtcctggct gtgttggtcg
     4141 tgatcggtct gctagtggtc ttgttgagcg gttttctgat taccaacacc ctccaggctt
     4201 tgttgaatca gcaagtggga cagatcggta tactgaaatc ggttggcgcc cggcgggttc
     4261 aaattgttgg tctctacatg atgctgagct tgttctacgg gcttctggcg ctggccattg
     4321 ccctgccgct ctcgtttgag gtggcatttc gcatcgttga tttcctgacc attcagatga
     4381 acaactcgtt ctttggtttt cgtatccttc cagaggtcat cctggttgaa gccgccattg
     4441 ccgttctgat gccgcagatt gctgcgctgg tacccttgtg gcagggcacc catatcagtg
     4501 tgcaagaagc cttgagcggt atccgccagg atcatccgct gggccggagc tggctagacc
     4561 ggagcatcag ccgcttgcgg aatttctcgc tgcttcttgt catgtctttg cgcaatactt
     4621 tccgtcgcaa aggtcgcctg gctttgaccc tcgtcaccct caccttaggg ggagcggtgt
     4681 tcatcgctac tttcaatgtg cgcgtttcac tgataatgta tattgaccgg atcatccagt
     4741 attttctggc agatgtcaat gtcacactga accaacccta ccgcatggat gaagtccgcg
     4801 ggctgatcat ggaagtcccg ggggtgaaaa tggtggaagg gtgggcattt gcccgtaccg
     4861 agctggtgat ggctgatggt tccgtaggcg aaagtgtctc attgctggca cccccggcag
     4921 acagccccct ggtgaagcca attattgtag caggtcgctg gatccagcct ggagaccaga
     4981 acgctatcgc tctcaacgag ttgttccgag aacgctatcc tgatctgcag gtgggcgaca
     5041 caatacagct tcaagtggac ggcgaagaga atgactgggt ggtggtaggt ttctatcaaa
     5101 tggctggtaa agtgagtggt ttttcagcct acaccagtta cgaatatctc tctgagctaa
     5161 atcaccagac cggcaaggcg atcagctaca gggtggtttc gagctggcag ggactggggc
     5221 gcgcccagca agaggcgctt ggtcgggcga ttgaagcgca tctggagggt gctggtctgg
     5281 atgttgtcga tgtgaccacg ggtatgtctc tgagcgagac cgcttccaac ggcttcaatg
     5341 tcctcacggc tttcttgctc tttttggcgc tgctgactgc cgtggtgggc agcatcggcc
     5401 tgaccgggac aatgagtctt aacgttatgg agcgcacgcg tgaaattggt gtgttgcggg
     5461 cgatcggggc atccaaccgc accctcatgc aaatggtgct ggtggagggg gtgttgatcg
     5521 ggttcctgag ctggatactg gcctcgctgg cggcctttcc catcagcgta ttaattgccg
     5581 aatcgatcag tcaatccctg ttcggggcgt cttccaagtt tggatttacg cctatcgggt
     5641 ttgtgatctg gatgctcgca gtattggtgc tttcggtgtt ggcgagcgtc atccccgccc
     5701 gcaatgccac ccgtttgacc atccgggaag tgttggctta cgaataataa aaattgtaga
     5761 agtaacgcaa ccaataataa gagagtgggg caggcattgc ctgccccact ctcttatttt
     5821 ttacttacgt aagtcgcaga ctgatagagg cgacgacggg atttgaaccc gtgatcaggg
     5881 ttttgcagac ccttgcctta ccacttggcc acgtcgccaa cctaattgag gattgtagat
     5941 tgaagattgc tgattgatca tcgtcaatca gcaatcggca acgaaaaatc ttcaaccagg
     6001 cagagcgggc gatgggattc gaacccacga ccttctcctt ggcaaggaga cgttctacca
     6061 ctgaaccacg cccgcgtatc cggccatttt ataggccgag agtgccgaga gccagaatcg
     6121 aactggcgac accgcgattt tcagtcgcgt gctctaccaa ctgagctatc tcggcctgaa
     6181 tctacagctc aattcccaga gcacgcctat tttacctact aggtagggta ttgtcaagca
     6241 aaggacagga tcgct
//
LOCUS       DBNGSX010000431         7100 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_594356, whole genome shotgun sequence.
ACCESSION   DBNGSX010000431 DBNGSX010000000
VERSION     DBNGSX010000431.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7100)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7100
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_594356"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="AC16CB_12805"
     CDS             <1..230
                     /locus_tag="AC16CB_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343391.1"
                     /translation="ERLRLIRICLERLRFLASAAPLLVSASPDSGPPMSGGRGRRQLQ
                     PDDRLLALLEEAADQVWRFGPPPPTVQLRLL"
     gene            368..649
                     /gene="rpsF"
                     /locus_tag="AC16CB_12810"
     CDS             368..649
                     /gene="rpsF"
                     /locus_tag="AC16CB_12810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR3343392.1"
                     /translation="MREYEYIFIIHPDLDENAFKELVDKIKGWITGAGGQVVKVDLWG
                     KRKLAYPIRKQTEGQYVLLDTVMSPDYSAELERNVRFLEPILRYLVTLK"
     gene            684..1079
                     /locus_tag="AC16CB_12815"
     CDS             684..1079
                     /locus_tag="AC16CB_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR3343393.1"
                     /translation="MSRGLNKVMLIGRLGRDPEMRYTPSGRPVTTFSLATSRTWNTSE
                     GERRTETEWFNIVAWGSLAEICKQFLNKGQQVYVEGRLQTRHWEDAEGSKHSVTEIVA
                     NEMIILGDRREMNNAAGEPGAEEEDEFPF"
     gene            1098..1370
                     /gene="rpsR"
                     /locus_tag="AC16CB_12820"
     CDS             1098..1370
                     /gene="rpsR"
                     /locus_tag="AC16CB_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR3343394.1"
                     /translation="MSNQFDMKDQMQSGPRRYYAHPRVCQFCTDHNVTIDYKNVDLLR
                     RYVTEEGKIRPRRQTGTCARHQRELARAIKRARHLALLQFTGEILR"
     gene            1439..2863
                     /locus_tag="AC16CB_12825"
     CDS             1439..2863
                     /locus_tag="AC16CB_12825"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3343395.1"
                     /translation="MAKNPRQKQTFVTKKHLARQQREQRSIRMITISAIVVIVAVIGL
                     IGFGVLNERVLKGLQSVAIVNGEKITTNEFQAQARLARQNLVDNALSTYQFAQQFGNN
                     PQFLSSLSSQLSQIQSQLEPVILGNQVLEQMIQDRLIRQEASRRGITVSQEEIEQSVQ
                     EAFGYYQNGTPTPEPTLAEKPTSTLSSLQMTLTAPTATLAFSPTLAATQAFTPTATLE
                     PTVTPTATATITPIPSPTPTQAPTATPTEYTLEGYQQLYKDQLERLKSIDFDEDDLRY
                     IIESQLYREKVLDAVLAESDLTAEEDQVWARHILVPDEATANIVLGRLADGEDWSELA
                     ALFSTDTSNKDFGGDLGWFGKGRMVAEFENAAFALDVGETSQPVQTSFGWHIIQVLGH
                     EARPLSQDEYEQLRQTRFDEWIKEQRDATEVETLEIWQERVPTEPAFPAEVEGFIQAV
                     QQQLLQQSQPTVPAPSVTEEAPTQ"
     gene            3049..4533
                     /locus_tag="AC16CB_12830"
     CDS             3049..4533
                     /locus_tag="AC16CB_12830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3343396.1"
                     /translation="MKHAVIIGAGMGGLATALRLSYHGFRVTVLEKQPRPGGRSNVIE
                     ESGFRADMGPTILVMKGAFEELYRSIGQDLNSRLQFIQLDPNYRVYYHDGSCIDLYSN
                     MAKLAQEIERVQSGGAERLFRFVGEGAKKYQLGMDFVDRNYDQLTDLMNPSAAVRLLQ
                     TQAHQNLYQQVSAFFQHNDKLAKAFSFHSMFLGLSPFDALAMYSLITYADLALGMWFP
                     MGGLYRLIEDMVSLAGEMGVEIRTESPVEEITIVDGRVTGVRLESGEQVASDLVISNA
                     DLPYTYNKLIAPRYRKQYSSARLAKMQYACSGYILYLGVNRIYPHARHQALYFSEDYR
                     ANLDAIFKHKTLPEDPSFHLNIPTVTDLNLAPPGHSLIYVLAPMPNLEAKIDWQQAAP
                     VVRQRLLDRLEQILDPEIRQHIVWERDYRPTDWHSDINATLGTAFGSLAHGFFQSAYF
                     RPHNKAKGVQGMYFVGQGTYPGIGMPMVVISSKLVTERVLKEWM"
     gene            4524..5468
                     /locus_tag="AC16CB_12835"
     CDS             4524..5468
                     /locus_tag="AC16CB_12835"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HIR3343397.1"
                     /translation="MDVDVNLSLPTVRSVREGEINSFKESGSIASVADYAACRAIMLR
                     ASNNYSSASRYLPADKLLHVEALYALMRVGDDRVDVSHQGFRTPLEAIEDWEKTYWLA
                     FERGESPHPVMRAYLDTAIKFGIPAETMSAYFRAMKEDLTVTRFPTFNHLLHYMEGSA
                     IPVGRAMTHILGVREPYSIGEALPGADSLSTAMQLSNFWRDIGEDWAIDRVYLPLEDL
                     QRFGYSLSDLGAQRIDERLIALLEFQFERTEKYYQRARTSVDMLNSGQWAVMTALEVY
                     RALLGDIRSHGYNVFSRRAGTSNLKKIGLAIKAYWAIR"
     gene            5471..5947
                     /locus_tag="AC16CB_12840"
     CDS             5471..5947
                     /locus_tag="AC16CB_12840"
                     /inference="COORDINATES: protein motif:HMM:NF047191.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6992 family protein"
                     /protein_id="HIR3343398.1"
                     /translation="MTRFPTDKRAFNLFRFQMEALPVLMGWAAGSIAAGLLWLRSKSA
                     VMAGIGGQFVLWGAIDGLIAALGLRGAAGGMRRLAKKEMSEADHLQQAERFERFVWLN
                     AGLDVLYIMGGGWLAQRSRNDDRRRGMGIGIVLQGGFLLVWDVILALLVRGKRRDA"
     gene            5937..6758
                     /locus_tag="AC16CB_12845"
     CDS             5937..6758
                     /locus_tag="AC16CB_12845"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3343399.1"
                     /translation="MTLNLRPYSGAEHQPFEMGDGKSGFLLLHGFTGTPAEVRGLGET
                     LAQLGWHSHAPLLPGFGSDIINLDQRRRADWLDAARDVWEKLHHRYQPSVLFGYSMGG
                     AIAIHLACELSPDILVLAAPFWRMPGWMPRLIPVARIFTPHMRPFKKADFSDVRLRRE
                     FERIMPGIDLDDTEVQESIRAEFTLPLTALHEAMKLGSSAYKLAKRVQCPVLVLQGEQ
                     DTIVRSDQTRKLFRRIGSPRKSYQSIPTGHDLLMAGTPQLEQTVNTVLGFIESIR"
     gene            6755..>7100
                     /locus_tag="AC16CB_12850"
     CDS             6755..>7100
                     /locus_tag="AC16CB_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014630567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HIR3343400.1"
                     /translation="MTYFRVLAVFLIPPLLGLLAIVPTDVWRWLRDRSTLVHRRAYLV
                     ILIHVLLALVYTTPWDNYLVATGVWTYPPELVSGVILGWVPLEEYLFFVLQTLTTGLW
                     ALAMRRIFPRKQA"
BASE COUNT         1669 a         1736 c         1972 g         1723 t
ORIGIN      
        1 gggagcggct tcgcctgatc agaatttgtc tggagcgttt gcgcttcctg gcttcagctg
       61 caccgttgct ggtgagcgcc tcgcccgata gcggtccgcc gatgagcggg gggcgcggtc
      121 gacgccaact tcagcccgat gatcgactgc ttgccttgct ggaggaagcc gccgatcaag
      181 tgtggcgctt tggccccccg ccgcccactg tgcagttgag attgctctag caattatccc
      241 tatgcaaacc gcaccctttg ggttatacta ttgacaggag ccacgcagat gcgtggctta
      301 ctttccgctc ctgaccggct cccagccgag tttaggggca aacccgtgga aaggaggcat
      361 cccctgcatg cgcgagtatg aatacatctt cattatccac cccgatctgg acgagaacgc
      421 atttaaagag ctggtcgata agatcaaggg ctggatcact ggagccggtg ggcaggttgt
      481 gaaagtggac ttgtggggca agcgcaaatt ggcttacccc atccgcaagc aaacagaggg
      541 ccagtacgtg ttgctagaca cggtaatgtc ccctgattac agtgcggagc ttgagcgcaa
      601 tgtccgtttt cttgaaccca tcctgcgcta tctggtgact ttaaagtaag tagttgtgta
      661 ggtgtcactt gaaggagtag aaaatgagcc gaggcttgaa taaagtgatg cttattggtc
      721 gcttgggtcg ggatccagag atgcgttata cgccgtctgg cagacctgtg accaccttca
      781 gcttggctac cagccgcacg tggaacactt ctgaaggcga acgacgtaca gagaccgagt
      841 ggtttaacat cgttgcctgg ggtagcctgg cagagatttg taagcagttt ctcaataaag
      901 gccaacaagt gtatgttgag gggcgcttgc aaacccgcca ttgggaagac gctgaaggga
      961 gcaagcattc tgtcacggag atcgttgcca atgagatgat catacttggc gaccgccgcg
     1021 agatgaacaa tgcggcggga gagcccgggg cggaagagga agacgaattt cccttttaag
     1081 aataaggagt tttaatcgtg agtaaccaat ttgatatgaa agaccagatg caaagcgggc
     1141 cgcgacgtta ctatgctcac ccacgcgtgt gccagttttg tactgaccat aacgtcacta
     1201 ttgattacaa gaacgtcgat ttgctgaggc gttacgttac tgaagagggc aagatccgcc
     1261 cgcgtcgaca gacaggcacc tgtgctcgtc accaacgcga gctggcgcgt gccatcaagc
     1321 gcgcccgcca cctggctctg ctccagttta ctggcgaaat cctgcgctga aatccgaatc
     1381 tccacgaagc gtcagatggg tgatttagac actggcgctt ctttcttgta gatttaacat
     1441 ggcaaaaaat cctagacaga aacaaacgtt tgttaccaag aaacatcttg cccgccagca
     1501 gcgcgagcaa agatcgattc gaatgatcac catcagcgcg attgtggtaa ttgtggctgt
     1561 gattgggttg atcggttttg gcgtgctcaa tgagcgcgtg ctgaaaggtc tccagtcggt
     1621 ggcgatagtt aatggagaga agatcaccac aaacgaattt caggcgcaag cgcgcctggc
     1681 gcgacaaaat ctagttgaca atgcgctcag cacataccag tttgcgcaac agttcggcaa
     1741 taaccctcaa tttctctcat cgctgtcgag ccagttatcc caaatccagt cgcagcttga
     1801 gcccgtgatt ttgggcaacc aggtactgga gcaaatgatc caggatcggt tgatccgaca
     1861 ggaggccagc cggcgtggta tcactgtgag tcaagaggag atagagcaat ctgtccaaga
     1921 ggcttttggt tattatcaaa atggcacacc tacacccgag cccaccctgg ccgaaaaacc
     1981 cacctcaaca ctctcctcat tgcaaatgac cctgacggct cccacggcga ccctggcttt
     2041 ctcgccaacg ttagctgcca cgcaggcttt tacacccacg gcgaccttgg aacctaccgt
     2101 cacacccacc gctacggcaa ccatcacgcc tattccaagc cccactccaa cgcaagcgcc
     2161 gacagcaaca cccaccgagt atacgctgga gggttatcag cagctctata aggaccaact
     2221 cgagaggctg aagtcgattg attttgatga agatgatctg cgatatatca tcgagagcca
     2281 gctctatcgc gagaaagtgt tagatgcggt tcttgcagaa tctgatttaa cagcggagga
     2341 ggatcaggtg tgggcccggc atatcctggt gcctgatgag gctacggcta acatcgtgct
     2401 gggtcggctc gcggatgggg aagactggag cgagctcgca gccctgtttt ctaccgatac
     2461 gagcaataaa gattttggtg gcgatcttgg atggtttgga aagggtcgca tggtggctga
     2521 attcgagaat gctgcctttg ctttagatgt tggtgagaca agtcagccag tgcagaccag
     2581 cttcggctgg cacatcattc aggtgcttgg tcatgaggcg agaccactct cacaagatga
     2641 atacgagcag ttacgtcaaa ctcgatttga tgaatggatt aaagaacaac gcgatgctac
     2701 cgaggttgaa accttggaga tttggcagga gcgcgtcccc accgagccgg ctttcccggc
     2761 agaggtggag ggctttattc aagccgttca acagcaactc ttacagcaat ctcaacccac
     2821 tgtgccagct ccttcggtga cggaggaagc cccgacgcaa tagctggaaa ataattttcc
     2881 tacaattgct ctctatgtcg aataaattgg gtgtgaattg cttgttcaca cccagttttt
     2941 ttataacggc gagatcgaga ggtcttatgt acaataaatg tcaataatat attgacatta
     3001 tatgtgcaat aggatagaat ttatctatct tcatcaaagg aaatggctat gaaacacgct
     3061 gtgatcattg gcgcgggtat gggggggctg gctacggcct tgcggctgag ttatcacggc
     3121 tttcgtgtta ccgtgctgga aaaacaaccc cgtccaggtg ggcggagcaa cgtcatcgag
     3181 gagtccggtt tccgcgcaga tatggggcct accatccttg tgatgaaagg tgcctttgag
     3241 gagctgtacc gctccattgg tcaagatctc aattcccggc tacaatttat ccagctagat
     3301 cccaactacc gggtttatta tcacgacggg agctgtattg atttgtacag caacatggct
     3361 aaattggcgc aggagattga aagggtgcaa tcggggggtg cagaacgcct tttccgtttt
     3421 gttggtgagg gagccaaaaa ataccagctc ggtatggatt tcgtagaccg caattatgat
     3481 cagttaactg acttgatgaa tccatctgct gcggttcgtc tgttgcagac gcaggcgcac
     3541 caaaacctgt accaacaggt gtcggctttc ttccagcata atgataaact cgccaaggct
     3601 ttctcttttc actcgatgtt tctggggctc tcaccattcg atgcgttagc gatgtatagc
     3661 ctgattacgt atgccgatct ggcattaggt atgtggtttc ccatgggagg cttatatcgt
     3721 ttgatcgaag atatggtcag tctggcaggt gagatggggg tggagatacg caccgagagc
     3781 ccagtagaag aaatcaccat tgtggatggt cgagtgaccg gtgttcgttt ggaatctggg
     3841 gaacaagtgg catctgacct ggtgatttcg aacgccgatc tcccctacac gtataacaaa
     3901 ttgatcgctc cccgctaccg caagcaatat agttccgctc ggttggcgaa aatgcagtac
     3961 gcttgctctg gttacatttt atacctagga gttaaccgga tctaccctca cgcccgccac
     4021 caagctctat acttctcaga ggattaccgt gcaaatctgg acgctatctt caaacacaag
     4081 actttgccag aagatccctc gttccacctg aacattccaa cggtcacaga cctcaattta
     4141 gcgcctcctg ggcacagctt aatatatgtg ctggcgccca tgcccaacct ggaggcaaaa
     4201 atcgactggc aacaagccgc gccagttgtc cgccagcgac tgctggacag attggagcag
     4261 atcctggatc ccgagatccg acagcatatc gtctgggaga gggattaccg gcccacagat
     4321 tggcactcag atatcaatgc caccttgggg acggcatttg gctcgctagc acatggcttt
     4381 tttcaatccg catattttcg cccacataac aaggctaagg gtgtgcaggg gatgtacttc
     4441 gtaggtcaag ggacttatcc ggggatcggc atgccgatgg tggtgatctc ttcgaagttg
     4501 gttacagaaa gggtgctgaa agaatggatg tagatgtcaa tttatcatta ccgactgtgc
     4561 gatcagttcg tgaaggcgaa atcaatagct ttaaggagag tggctcaatc gcttctgtag
     4621 cagattatgc ggcttgtcgc gcaattatgc tccgggcgag taataattat tcatctgcca
     4681 gcaggtattt gccggctgat aagcttctgc atgtcgaagc gttgtatgcc ttgatgcgcg
     4741 tgggagatga ccgcgtggat gtatcacacc agggatttcg cacaccgttg gaggcgattg
     4801 aagattggga aaagacatac tggttagcgt ttgagcgggg cgaaagccca catccagtaa
     4861 tgcgagccta tctggacacg gcgataaaat ttggcatccc tgcagagacg atgagcgcat
     4921 acttccgcgc catgaaggaa gatctgaccg taacgcgctt ccccacgttt aatcaccttc
     4981 tgcattacat ggagggcagc gctataccgg tagggcgggc aatgactcat attttaggag
     5041 tacgtgagcc atactctatc ggcgaggctt tgccaggtgc agacagcctg tctacagcca
     5101 tgcaattgag taatttctgg cgagatattg gcgaggattg ggcgatagac cgcgtttact
     5161 taccacttga agatctacag cgctttggat acagcctctc ggatctgggt gcacagcgca
     5221 ttgatgagcg gttaattgcc ttgctggagt tccaatttga gcgtactgaa aagtattacc
     5281 aacgcgcccg cacgagcgtg gatatgctga attcagggca atgggcggtt atgacggctc
     5341 tggaagtgta ccgagcgctc ttaggggata tacgctctca cggatacaat gtgttctcgc
     5401 gacgagcagg aaccagcaat ctcaaaaaaa tcggcttggc gataaaggct tattgggcga
     5461 ttcgctgatc atgactagat ttcccactga taagcgagcc ttcaacctgt ttcgtttcca
     5521 gatggaggct ctacctgtat taatgggttg ggcagctggc tcaattgcag ctggtttgct
     5581 ctggttgcgc tcaaaaagcg cagtgatggc tggtataggt ggtcaattcg tcctttgggg
     5641 agcgattgac ggattgattg cagccttggg tttgcgcggc gcggctgggg gaatgcggcg
     5701 tctggctaag aaggagatga gcgaagcaga tcatctccag caagcagagc gctttgagcg
     5761 ctttgtatgg ttaaacgcag gattggatgt gttgtatatt atggggggtg gctggctggc
     5821 gcaacgcagt cgcaacgatg acagacgacg cggtatgggc ataggtattg tgttacaggg
     5881 tggatttttg ctggtatggg atgtaatcct ggctttgctg gtgaggggca agcggcgtga
     5941 cgcttaacct gcgaccctat agtggagcgg aacaccaacc ctttgagatg ggtgacggaa
     6001 aatccggatt tttgctgttg catggcttca cgggcacacc cgccgaggtg cgcggcctgg
     6061 gggagacact tgcccagctg ggttggcatt cccatgcacc gctcctgcca ggttttggta
     6121 gcgacatcat caatctcgac cagcgccgcc gcgcagactg gctggatgca gcccgtgatg
     6181 tgtgggaaaa attgcatcac agataccagc cgagtgtgct ctttggatat tcgatgggcg
     6241 gagcgatcgc aatccatctg gcttgcgaat tatcccctga tatcctggtg ctagccgctc
     6301 ctttctggcg catgcctggc tggatgccga ggttgatccc ggtagcgcgg atatttaccc
     6361 cacatatgcg tccatttaag aaagcagatt tcagcgatgt ccgccttcgg cgcgagtttg
     6421 agcgaatcat gcctggaatt gacctcgacg atacggaagt gcaggaatct atccgggcgg
     6481 agtttaccct tccactaact gccttacacg aggctatgaa acttgggagc agcgcgtaca
     6541 agctggcaaa gcgagttcaa tgcccggttt tggtcttgca gggtgaacag gatacgatcg
     6601 tccgctctga tcaaacacga aaattattcc ggcgcatcgg ttcgcctcgt aaaagctacc
     6661 aatcgattcc caccggccac gatctcttga tggcgggcac accgcagttg gagcagacgg
     6721 ttaatactgt gctcggattt atcgaatcaa ttcgatgact tattttcgcg ttctggctgt
     6781 ttttctgata cccccgctgc tgggtttgct agcgatcgtg cctacagacg tgtggcgctg
     6841 gctgcgcgac cgttcgaccc tcgtacatcg cagggcttat ctagttattc tcattcatgt
     6901 tttactggct ttagtctata ccactccatg ggataattac ctggtcgcca caggcgtgtg
     6961 gacctatccc cctgaattgg tcagtggggt gatactgggg tgggtgccgc ttgaagagta
     7021 tctgtttttt gtgttgcaaa ccctaaccac cggattgtgg gcattagcca tgaggcggat
     7081 tttcccgcga aaacaggcga
//
LOCUS       DBNGSX010000432         1656 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1910795, whole genome shotgun sequence.
ACCESSION   DBNGSX010000432 DBNGSX010000000
VERSION     DBNGSX010000432.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1910795"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="AC16CB_12855"
     CDS             <1..397
                     /locus_tag="AC16CB_12855"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR3343401.1"
                     /translation="FVVGFLGIGLGPDSAVSLLLPATIGLGRATEYAFTNAPINAEQA
                     LELGLVNRVVTADELSQEAFKWASALAQGPVGAFGLTKRAFNKSVLSNLEQVLDYEAH
                     IQDIAGRSDEHKEGVEAFLEKRSPNYVNS"
     gene            879..1100
                     /locus_tag="AC16CB_12860"
     CDS             879..1100
                     /locus_tag="AC16CB_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343402.1"
                     /translation="MLERRNQSDLNYIKAHHRELIQAAQQEKLLGQTKDSDPNLKERL
                     LDAAGNRLIAWGQKLKGETYSNELSQECV"
     gene            1114..>1656
                     /locus_tag="AC16CB_12865"
     CDS             1114..>1656
                     /locus_tag="AC16CB_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343403.1"
                     /translation="MSDRDKTPKGQPRLGWDSGSAYDFFFSLEVLHNPDQYGLRASWA
                     AGVRSRLPTQERKLLEEIQPYLGVPLNWIYRLPEPKDATSAIWALRQTPPAERMVNLA
                     GYCDMEDLAVIYKQVAERRSWDNNDVKVIHEILNQHKKEKRYVSRFLKDLPKCLDWWS
                     RPDEFGELYLSALQTFFQVFF"
BASE COUNT          443 a          340 c          438 g          435 t
ORIGIN      
        1 cttcgtggtt gggtttctgg gtatcggctt gggccctgac tcggcagtct cattgttgct
       61 gcctgcgacg attggcttgg ggcgagcaac ggaatacgcc ttcaccaatg cgcccataaa
      121 tgcagaacaa gccctagagc taggactggt caaccgcgtg gtgacagccg atgaactaag
      181 ccaagaagca ttcaaatggg cttctgcatt agctcaagga cctgtgggtg catttggtct
      241 caccaaaaga gctttcaaca aatcggttct aagcaacctg gagcaggtgc tggactatga
      301 agctcatata caagatatcg ccggacgaag tgatgaacat aaagaaggtg ttgaagcttt
      361 tctggaaaaa cgatctccga actatgtcaa tagttaatca ttatcattat gtattattta
      421 tatatcgtag tcttagtagg ctgggtggat aagtggataa agtaataacc taagtgcttt
      481 actggcatat atgggggtga tggatcagag gtcggggaga tatagtacaa gacaaacggc
      541 attgtatcca cagggtggat ggggatagat gcgaagaaga ttttttgcat gatacccttt
      601 tgggaggggg gatgatattt agggtgcaat tctgttggga ccccatatat ggggctttgt
      661 caaatttcaa aattcgcaca aaagatcgag ttttccccat taagtgcgtt ttatccacag
      721 tttttcacga gttatccaca ggttgagcct ctaaaaagtg tcgctccgga aaatttgtgt
      781 cttgacaaaa cgatggagtt agactattat ctaattagat aaatatctac gttagttata
      841 tgtctatatt agatagatgt ctattgaagg agaacgccat gttagagcgc cggaatcaga
      901 gtgatttgaa ttacatcaag gctcatcatc gtgagctcat tcaggctgca cagcaggaaa
      961 aactgctagg gcaaacaaag gacagcgatc ccaatttgaa ggagcgcctt ctcgacgcgg
     1021 cgggcaaccg cctgattgcc tggggtcaaa agctgaaggg tgagacttat tccaatgaat
     1081 tatctcaaga atgtgtatag ggggtagcat tatatgagtg atcgggacaa aacaccgaaa
     1141 ggtcagcctc ggctgggatg ggatagcggc tcggcttacg atttcttttt cagcctggaa
     1201 gtgctgcaca acccggatca gtatggtctg cgcgcctcct gggcagccgg agtcagatcg
     1261 cgtctaccca cccaagagcg taagctcttg gaagaaattc agccctactt gggggtgccc
     1321 cttaactgga tttatcggtt gccggaaccc aaagatgcca ccagtgcgat ctgggctttg
     1381 cggcaaacac cgccagcgga gcgcatggtg aatctcgcgg gttattgtga tatggaggat
     1441 ttggccgtaa tttataaaca ggtcgctgaa cgccgttctt gggacaataa cgatgtcaag
     1501 gtgatccatg aaattctaaa tcagcataaa aaagagaagc ggtatgtttc tcgctttctc
     1561 aaggatctcc ccaagtgcct ggattggtgg tcacgaccgg atgaatttgg cgagctttac
     1621 ctgtctgctc tgcagacctt tttccaggta tttttc
//
LOCUS       DBNGSX010000433         7689 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_525887, whole genome shotgun sequence.
ACCESSION   DBNGSX010000433 DBNGSX010000000
VERSION     DBNGSX010000433.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7689)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7689)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7689
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_525887"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /gene="ilvD"
                     /locus_tag="AC16CB_12870"
     CDS             <1..1248
                     /gene="ilvD"
                     /locus_tag="AC16CB_12870"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR3343404.1"
                     /translation="GQAVDLISVFEGVGAYQAGRIDAARLKLLEDFACPSCGSCSGMF
                     TANSMNCLMEALGIALPFNGSALAQTPEREALASRAAELILELVERDITPRQIVTAEA
                     LDDAFALDMAMGGSTNTVLHALAIAHEAGVDFPLSRINHVADQVPHLCKVSPAGKWHM
                     EDVHRAGGIPAILTEIERNSDLLHLDRITVSGKSLRESIAGAAVQDSEVIRPYAEPHS
                     RHGGLAILFGNLAPKGAVVKTGGVSPAMKQFTGQARIYESQENAMTGIMAGQVKPGDV
                     VVIRYEGPKGGPGMVEMLSPTSAIMGMGLGDKVALITDGRFSGGTRGACIGHVSPEAA
                     ARGPIAALQQGDVIDIDLEKRTLDVRLTDEEIQRRLESLPAFQSRTRSKWLRRYSYFV
                     SSADTGAVLMDTAEAQTSPSFVR"
     gene            1321..3039
                     /gene="ilvB"
                     /locus_tag="AC16CB_12875"
     CDS             1321..3039
                     /gene="ilvB"
                     /locus_tag="AC16CB_12875"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HIR3343405.1"
                     /translation="MKRTGAQIVWETLVREGVDLVFGYPGGANLPIYDAMLGYPVRHV
                     LARHEQGAAHMADGYARASGKVGVAMATSGPGATNLVTGIATAIMDSSPMVCITGQVV
                     SRFIGYDAFQETDVTGITLPITKHNYLVTDVQEIGEVLREAFYIARTGRPGPVLVDIA
                     KDAQQASIEWEYDDSPIRLPGYRPDLRPVTEDIQKALELIYNAKRPVILAGHGVLLSG
                     AMAELREFAERAQTPVAMTLLGLSGMPSDHPLNLGMMGMHGEAWVNHAIQESDLLLAF
                     GMRFDDRVTGNLKTYAPNAKKIHIEIDPSEINKNVTVDVGLIGDLRETLSWILEHLEP
                     CDKSEWLTYIDDMKGDSAVRDIQNLPDSGVLYAAHVINDLWRYTDGKALITTDVGQHQ
                     MWTAQYYKFNTPGNLITSGGLGTMGFGLPSAIGAKFAKPDAQVWTVVGDGGFQMTQAE
                     LATAAQEGVKVNVAIINNGYLGMVRQWQEFFYERRYAATPMRNPDFVKIAEAHGLTGL
                     RVTQRSEVEAAIRSAQEIEGTVVIDFKVEQEDSVYPMVPAGADLHNMIRRPVSNPLVE
                     TAQDTV"
     gene            3054..3587
                     /gene="ilvN"
                     /locus_tag="AC16CB_12880"
     CDS             3054..3587
                     /gene="ilvN"
                     /locus_tag="AC16CB_12880"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIR3343406.1"
                     /translation="MQHTLVVLVEDKPGVLSRVASLFRRRAFNIESLTVGHTDQPGVS
                     RMTIVVDSDDTSVERLTAYLYKLVNVIQVHDMTTVPTVSRDLAMIKVNAPAENRTHIM
                     QTVDVFRARILDVTNNSFIIEVTGDEEKINGFVEVLKPLGIIEMVRTGKVTMARGASP
                     LPDANGNGHRPAAEMMV"
     gene            3648..4700
                     /gene="ilvC"
                     /locus_tag="AC16CB_12885"
     CDS             3648..4700
                     /gene="ilvC"
                     /locus_tag="AC16CB_12885"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIR3343407.1"
                     /translation="MAWMDFCGTQEEVVTREEFPLEKAREVLKDEVIAVLGYGVQGPA
                     QALNMRDNGFNVIVGQKPGGKSWEKAVKDGWVPGETLFSLEEATERGTVIQYLISDAG
                     QRLMWPKIKPCLNEGDALYFSHGFSVVYQDQTGIVAPEYVDVILVAPKGSGASVRTNF
                     LNGSGINSSFAVEQDYTGRARERTLALGIAIGSGYLFATTFKHEVYSDLTGERGVLMG
                     ALAGIMEAQYQVLRENGHTPSEAFNETVEELTQSLIRLVAQNGMDWMYANCSTTAQRG
                     ALDWRHQFKDAVDPVFQRLYESVAAGEETRIVLNSNSAPDYKEKLDAELQEMRNSELW
                     QAGALVRSLRPENWQQ"
     gene            4812..6440
                     /locus_tag="AC16CB_12890"
     CDS             4812..6440
                     /locus_tag="AC16CB_12890"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIR3343408.1"
                     /translation="MTDNYVRFFDTTLRDGEQSPGATMTSAEKLEVARALARLGVDII
                     EAGFPAASPDDLEAVRRIAIEVGNPSDGTRPPVICGLARTTRGDIDKAWEAIRHAAHP
                     RIHTFLATSDIHMKHKLKMTRRQVVERVGEMVAYARSLCEDVEFSPEDAGRSDPEFLY
                     QVLEVAIKAGATTLNIPDTVGYTLPEEFGALIAGIIQNTPGIQNCIVSVHCHNDLGMA
                     TANTLAGIRAGARQAEVTINGIGERAGNTALEEVVMAIGTRPNQFNLLTGIDTTQIAR
                     VSKLVSNYTGIVVQPNKAVVGANAFAHEAGIHQDGMLKHNLTYEIMRPETVGASVSKL
                     VLGKHSGRHALKARLTSMGYFLDEAEVDKLFERFKALADKKKTITDADLEALISDELY
                     QPRELFSLEGLQVACGTMGMPTASVRLRDPLGKLHVQASIGTGPVDATYKAIDAIVQA
                     PNILQEFVVHAVTEGIDALGEVTVRIQGEQPSLMLDAQTEVDQPRTFGGYGADTDIIV
                     ASAKAYLSALNKMLVSMGHFGPVEIAATETINQS"
     gene            6451..6654
                     /locus_tag="AC16CB_12895"
     CDS             6451..6654
                     /locus_tag="AC16CB_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343409.1"
                     /translation="MNPKDKSTPSRSLDPSVDLRTIPSGWDLSGLFSPADNWVNEPQP
                     YQDRSHINPDSTGIPSDNAADAV"
     gene            6635..>7689
                     /locus_tag="AC16CB_12900"
     CDS             6635..>7689
                     /locus_tag="AC16CB_12900"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR3343410.1"
                     /translation="MPQTLFEKIWNQHLVEQEPGLPAVLYVDLHLIHEVTSPQAFTGL
                     RARKLPVRRADKTIATIDHAVPTLVNLDGDDGRLAYADEMSRRQIHMLEDNCREFGIR
                     LYGLDSPNQGIVHIIGPELGLTRPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLA
                     TQCLLQRRPKTCEVRIDGTLPPGVTSKDIILALISKIGVGGGTGHVFEYSGSAIRALT
                     MEQRMTLCNMSIEAGARAGMVAPDDTTFEYLSGREFAPRGQAWESSLRWWRELSTDDG
                     ATFDRTISLDASTLEPMITFGTNPGMGIPVNGHVPTLDEVKDATQKVALEKALNYMGL
                     SPGQTLVGHPVDVVFIG"
BASE COUNT         1727 a         1979 c         2329 g         1654 t
ORIGIN      
        1 ggtcaggccg tggacctgat ctcggttttc gagggagttg gtgcatacca ggcgggtcgc
       61 atcgacgctg ctcgcttgaa gctgctcgag gattttgctt gcccctcctg cggctcatgc
      121 tcaggcatgt tcactgccaa cagcatgaac tgcttgatgg aagccctggg tatcgccttg
      181 cccttcaacg gttcggcgct ggcgcagaca cccgagcgtg aagcgctggc aagccgggct
      241 gcggaactca tcctggagct ggtcgagcga gacatcactc cgcgccagat cgttactgct
      301 gaggcgctgg atgacgcgtt tgccctggat atggccatgg gcggctcgac gaatacggtt
      361 ttacatgcgc tggcaattgc ccatgaagcc ggtgtggatt tcccgctaag ccgcattaat
      421 catgtggcag atcaggtgcc gcatttgtgc aaggtcagcc cagcgggcaa atggcacatg
      481 gaggatgtgc accgggctgg gggtatacct gccatcttga ctgaaattga acgcaactct
      541 gacctgttgc acctagatcg tattactgtc tctggaaaga gcttgcgtga gtcgattgcc
      601 ggtgctgcag ttcaagacag cgaagtcatc cgcccctatg cagagccaca ctcccgccat
      661 ggtgggctgg ccatcttgtt tggcaacctg gcgccgaagg gtgcggtggt caagaccggt
      721 ggcgtcagcc cagcgatgaa gcaatttaca ggccaggctc gcatctatga atcccaggaa
      781 aatgcgatga cgggcattat ggcagggcag gtcaaacctg gcgatgtggt tgtcatccgc
      841 tatgaaggcc cgaagggtgg gccagggatg gtggagatgc tcagcccgac cagcgccatc
      901 atgggtatgg gcttggggga taaggtagct ttgatcaccg atgggcgctt ttctggtggg
      961 acacggggcg cgtgcatcgg gcatgtctca cctgaagcgg ctgctcgcgg cccgattgct
     1021 gcgctccaac agggagatgt tatcgatatt gacctggaga agcgcacgct ggatgtacgc
     1081 ctcactgacg aggaaatcca gcgacggctg gaaagcttac ctgccttcca gtcgagaacc
     1141 cgcagcaagt ggctgcgtag atattcgtat tttgtctcaa gcgccgacac cggcgcggtt
     1201 ttaatggata ccgcagaggc acaaacttcg ccgagctttg tacgataacg atctaggttt
     1261 tctgcatttt tcggtggtga atatggaata gccaaggttt tataaaaagg aatgaggcca
     1321 atgaagagaa caggtgcaca aattgtttgg gagaccctgg tgcgagaggg tgtggatttg
     1381 gtgtttggat atccaggcgg tgcgaacttg ccgatatacg atgccatgct gggttatccg
     1441 gtccgccatg tcctggctcg tcacgaacag ggtgcggcgc atatggctga tgggtatgcc
     1501 cgtgccagtg ggaaggtggg cgtagcgatg gcaacctctg gcccgggtgc gactaacctg
     1561 gtgactggca ttgccaccgc tatcatggat tcgtccccca tggtctgcat taccgggcaa
     1621 gtggtcagcc gctttatcgg ctacgacgct ttccaggaga cggatgtcac cggcattacc
     1681 ctgccgatca ccaagcacaa ttatctggta actgatgtac aggaaatcgg tgaggtcttg
     1741 cgcgaggctt tttacatcgc caggaccgga cgccccggcc ctgtattggt ggacatcgcc
     1801 aaggatgccc agcaggcctc cattgaatgg gagtacgacg attcgccgat caggctgccg
     1861 ggttaccgtc ccgatctgcg tcctgtgacg gaggatatcc aaaaagccct cgagctcatt
     1921 tataacgcca agcgtccggt tattctcgcc gggcatggtg tcctgctcag cggcgcaatg
     1981 gccgagttgc gggagtttgc cgaacgggcg cagaccccgg tggcgatgac tctcttagga
     2041 ttgagcggga tgccatctga tcaccccctc aacctgggca tgatggggat gcatggagaa
     2101 gcatgggtca accatgctat tcaggaatca gacctgttgt tagcttttgg gatgcgcttt
     2161 gacgaccgcg tgacgggcaa tcttaaaacg tacgcgccca atgcgaagaa gattcatatc
     2221 gagattgacc cgtcggaaat taataagaac gtcacggttg atgtcggctt gatcggtgac
     2281 ttgcgcgaga ccctgagctg gatactcgag cacctcgaac cttgcgataa atcggaatgg
     2341 ctgacttata tcgacgatat gaaaggcgac agcgccgtgc gcgatatcca aaatctgcca
     2401 gatagcggcg tcctgtacgc tgcccatgtg attaatgatc tctggcgcta tacggatggg
     2461 aaggcgctga taactacaga tgtgggacag caccagatgt ggaccgcgca atattataaa
     2521 ttcaacaccc cgggcaatct gatcacctcg ggcggcttgg gcaccatggg ctttggattg
     2581 ccttcggcga tcggcgccaa gtttgccaag cccgatgcac aggtctggac ggtggttggt
     2641 gacggtgggt tccagatgac gcaagccgag ctggcaacag ccgctcagga aggcgtcaag
     2701 gtgaatgttg ccatcatcaa caacggctat ctggggatgg tacgccagtg gcaggagttc
     2761 ttctatgagc gccgctatgc ggcaaccccc atgcgcaacc ccgattttgt caagatagcc
     2821 gaagcccatg gcctgacagg cctgagggtc acgcagcgct cggaagtaga ggctgcgatc
     2881 cggtcagcgc aagagataga aggcaccgtg gtgatcgatt ttaaggttga gcaggaggat
     2941 agtgtgtacc cgatggtgcc cgcaggcgct gacttgcaca atatgatccg cagaccggta
     3001 tctaacccgc tggtcgagac tgcacaggac acagtgtaag aggaggtgga gccatgcagc
     3061 ataccctagt cgtcttagta gaggataaac ccggtgtgtt aagccgggtc gcatcactgt
     3121 ttcgccggcg agcgttcaat atcgagtctt tgaccgttgg tcacacagac cagcctggcg
     3181 tctcgcgtat gaccatcgtg gtggacagcg atgacaccag tgtggagcgc ctgaccgcct
     3241 atttgtataa gctggtcaat gttattcagg tgcacgatat gaccacagtc cccacggtca
     3301 gccgcgacct ggcgatgatc aaggtcaatg cccctgcgga gaaccgtact cacattatgc
     3361 aaacggtgga tgtattccgg gcgcgcattt tggatgtcac caacaattcg ttcatcatcg
     3421 aggtgacggg agatgaagag aaaatcaacg gcttcgtgga ggtattgaaa cccttaggaa
     3481 tcattgagat ggtacgcacc ggtaaagtga ccatggcgcg cggcgcctcg ccgctgccgg
     3541 acgccaacgg gaatggacat cgccctgcgg ctgagatgat ggtatagcag aggtaattgt
     3601 tgggtcagat tgaccctaaa tacacacact agataaggag caatggaatg gcctggatgg
     3661 atttttgcgg tacacaagag gaagtggtta cgcgtgagga attcccgttg gagaaagcgc
     3721 gggaggtact taaggacgaa gtgatcgcgg tgttggggta tggcgttcag ggacctgcgc
     3781 aggcattgaa catgcgcgat aacggattta acgtgattgt agggcaaaaa cccggcggta
     3841 aatcttggga aaaagcggtt aaggatggtt gggtcccggg agagaccctg ttttcactcg
     3901 aggaagctac cgaacgcggc acggtgatcc agtatttgat ctccgatgcc gggcagcggc
     3961 tgatgtggcc gaagatcaag ccctgtctga acgaaggaga tgcattgtat ttctcgcatg
     4021 gcttctcggt cgtatatcaa gaccaaaccg ggatcgtagc tccagaatat gtggatgtca
     4081 tcctggtggc tcccaaaggc tcgggagcca gtgtgcggac taatttcctg aatggcagcg
     4141 ggatcaacag cagcttcgcg gtggagcagg actacaccgg tcgtgcacga gaacgcaccc
     4201 tggcattggg aattgcgatt ggctcgggtt acctgtttgc aaccacgttc aagcatgagg
     4261 tctacagcga cctgaccggt gaacgcggcg tgttgatggg cgcgttggcg ggcatcatgg
     4321 aagcccagta ccaggtgctg cgcgagaatg gacacacgcc gagcgaggca ttcaatgaga
     4381 ccgtcgagga gctgactcaa agcctgatcc gcctggtggc gcagaatggt atggactgga
     4441 tgtatgccaa ttgcagcacc actgcgcaac gcggcgcgct agactggcgt caccagttca
     4501 aagatgctgt ggatccggta ttccaacggc tgtacgagag tgtcgctgca ggcgaggaga
     4561 cgcgcattgt cttgaactcc aacagcgccc cggattacaa agagaagctg gatgccgaat
     4621 tacaagagat gcgtaattcg gagctttggc aggcgggtgc attggtgcgc tccctgcgac
     4681 ctgaaaactg gcagcaatga ttatcacttt agccatttga aactatgaag ccaaaggtta
     4741 agtgagtcag cgagatcata gcttgttgta gagagaactg ttttactggt attacaagcg
     4801 cggaggaata gatgacagac aattatgtac gattcttcga tacaacttta agggatggtg
     4861 agcaatcgcc tggtgcaacc atgaccagcg ccgagaagct ggaggtcgct cgcgccttgg
     4921 ccaggctcgg tgtggatatc atcgaggcag gttttccggc tgcctctcca gacgatctgg
     4981 aagcggtgcg ccggattgct atcgaggtag gcaatcccag cgacggcacc cgccccccgg
     5041 tcatctgtgg acttgcgcgg accacccggg gtgacatcga taaggcctgg gaagcgatcc
     5101 gccacgctgc ccaccccagg attcacacct tcctggcaac gtcagatatc cacatgaagc
     5161 acaagcttaa gatgactcgc cggcaagtgg ttgagcgggt gggcgagatg gtcgcctacg
     5221 ctcgcagcct gtgcgaggat gttgaattca gccccgaaga tgccggtcgc agcgatccgg
     5281 agttcctgta tcaggtgctc gaagtggcta tcaaagctgg cgcgacaacg ttgaatatcc
     5341 cggatacggt cggatacacg ctgccagaag aatttggcgc tttgatcgcc ggcattatcc
     5401 aaaacacccc tggcatccaa aactgcattg tttctgtcca ctgccacaac gatctgggga
     5461 tggcaaccgc aaatactttg gcaggtatcc gtgctggagc gcgccaggct gaagtgacga
     5521 tcaacgggat cggtgagcgg gcgggcaata ctgcactcga ggaagtggtg atggcaattg
     5581 gcacgcggcc caaccagttc aacctgctga caggtatcga taccacccaa attgcccgtg
     5641 tcagcaagct ggtgagcaac tacaccggta ttgttgtcca gccgaataag gctgtggtcg
     5701 gtgctaacgc cttcgcgcac gaagccggta ttcaccagga tggcatgctc aagcataacc
     5761 tgacttacga gatcatgcgt ccagagacgg ttggggcttc ggtgagtaag ctggtgctag
     5821 gcaagcattc cgggcgccat gcgctaaaag cccgcctaac atcgatgggc tatttcctgg
     5881 atgaagccga agttgataag ctcttcgagc gcttcaaagc cctggcagat aaaaagaaaa
     5941 ccattacaga tgccgatcta gaggcgctta tctcggatga gctttaccag ccgcgcgagt
     6001 tgttctccct ggaggggctg caagttgcct gcggcacgat gggcatgcca accgcctcgg
     6061 ttcgcctgcg cgacccgctg ggcaagctgc atgtgcaggc cagcatcggc accggtccgg
     6121 tcgatgctac gtacaaagcg atcgatgcca tcgtccaggc acctaatatt ttgcaggagt
     6181 tcgtcgtcca cgcggtcacg gagggcatcg atgccctggg agaggtgact gtgcgcatcc
     6241 agggtgagca acccagcctg atgctggatg cacagaccga agtagaccag ccgcgcacat
     6301 ttggcggtta tggagcagac acggatatca tcgttgccag tgctaaagca tatctgtctg
     6361 ctttaaacaa aatgctggtg tccatggggc atttcggccc ggtggagatt gcggcaaccg
     6421 aaaccatcaa ccagtcttga ggagaatgtc atgaacccta aggataaatc cactccatct
     6481 cgctcattgg atccgtcagt cgatctgcgc accatcccgt ctggctggga tctctccgga
     6541 ttattctcgc cggctgataa ctgggttaat gaaccccaac cctatcaaga tcgctcgcac
     6601 atcaatccgg attccaccgg gataccctct gacaatgccg cagacgctgt ttgaaaagat
     6661 ctggaaccag cacctggttg agcaggaacc tggcttgcca gctgtgctgt atgtcgacct
     6721 gcacctgatt catgaggtta cttcgccgca ggcatttacc gggttacgtg cccgtaaact
     6781 gcctgtgcgc cgggcagata agaccatcgc gaccatcgac catgccgtcc ctactctggt
     6841 caatctggac ggcgatgatg gcaggttagc gtatgccgat gagatgtcgc gccggcaaat
     6901 ccacatgtta gaggacaatt gcagggagtt tggcatccgt ctatatgggc tggatagccc
     6961 taaccagggc attgtccaca tcattggacc ggagttgggc ttgactcggc cgggcatgac
     7021 cattgtgtgc ggggatagcc atacggcaac ccatggggca ttcggtgcat tggcttttgg
     7081 aattggcacc agcgaagttg agcacgttct ggcgacccaa tgcttgctcc agcgccgccc
     7141 taagacctgt gaggtgcgca ttgatggaac gctaccccct ggggtgacct ccaaggatat
     7201 catcctggca ctgatcagca agatcggtgt gggcgggggg actggtcatg tttttgaata
     7261 tagcggttca gctattcgcg ctttgaccat ggagcagcgc atgactctct gcaacatgtc
     7321 gatagaagct ggagcgcggg ccggtatggt cgccccggat gatactacct ttgagtatct
     7381 ttctgggcgg gagtttgccc cacgcgggca ggcatgggag agcagcttgc gctggtggcg
     7441 ggaactatcc accgatgatg gcgccacttt tgataggacg atcagcctgg atgcctctac
     7501 cctggaacct atgatcacct ttggtactaa tccaggcatg ggtataccgg tgaatgggca
     7561 cgtccccacg ctggatgagg tcaaagatgc cacccagaag gtagccttag aaaaagcgct
     7621 gaactacatg gggttgagtc cagggcaaac acttgtcggg catcctgtag atgtggtctt
     7681 tattggaag
//
LOCUS       DBNGSX010000434         8106 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_41409, whole genome shotgun sequence.
ACCESSION   DBNGSX010000434 DBNGSX010000000
VERSION     DBNGSX010000434.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8106)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8106)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8106
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_41409"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="AC16CB_12905"
     CDS             <1..482
                     /locus_tag="AC16CB_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343411.1"
                     /translation="ILGVTAILVGLEGIIKRRIVLGSRYHRYGVETYLGLAATAQGML
                     FLFLGAFFVAVAVVAYLTTGRSLFLYFVRNPGLPLLIFGAGCFLAATVAMLGSVEQKQ
                     GAKWIILLDLLTSRLLPGLILIMLGLAATLLGLFEIVAPQAFDQIGGGFLEVLFSAR"
     gene            670..1872
                     /locus_tag="AC16CB_12910"
     CDS             670..1872
                     /locus_tag="AC16CB_12910"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR3343412.1"
                     /translation="MKYDMVFEGGGAKGMVFVGAMEVFKSAGHTHGRLLGTSAGAITA
                     SLLAAGYQPGEMLESLSEKIEGRSVFSDFMAVPSAPDEATLRASATRELLRNIDLPFI
                     PERFEEILDDQLVKWLASQPKLRSVFSFIERGGWYAADHFVTWLKRRLDSGSHEGKPR
                     QYSEMTLSEFYQATQTDLSLVASDTTQGAMLVLNHRTAPDLPLVRAVRMSMSVPLLWQ
                     EVVWQKGWGQSRDRDMVDHAIVDGGLLSNFPIELFVSRAASVTNVMGENVSQNVLGML
                     IDESLEVPGTEAAAKTPEKLSLGELRTVQRISNLVNTTLSARDKNVIDAFDKLVVRLP
                     AKGYGTTEFDMTDERRELLVNAGRTAMEAYFERPVPVELESFDIGATGDDLRAQQTAD
                     KTALKILE"
     gene            2193..3629
                     /locus_tag="AC16CB_12915"
     CDS             2193..3629
                     /locus_tag="AC16CB_12915"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR3343413.1"
                     /translation="MRNEKVSLSLRVTYLQKVFPLFLISLVIIYQLGLARWVHDHYSH
                     SLHYWVEILFYASVGPVLSFWILGLIRRWLDEKERAQQMARTSERYLASITRASADAI
                     FSLDPMGRIESWNRGAELIFGYEAGEILGRPLSDLFGGTDAAVVELRWLVESVVEAGF
                     VRGHETTCQDAAGHKVIAELTATRLADESGQILGLSIILRDITQRKQREEEIHRLNAN
                     LMELVALRTSELAEKVDQLAQANAELKQLDRVRSEFVSLVSHQLRAPLTNIRGAVERI
                     EDSCKMSNGTCMRMLTIIDQQINRLDKLVKDVLNATRIESGDLIIHAEPVSVWPVVAQ
                     LVEQMRSRASGRPLRLSEKPGLPLVYADRDRMAEVVMNLLDNADKYSPPGKEILVDLS
                     ADDSGVTVSVRDFGAGIPEGDFERVFEKFYRADNSDSQSVYGYGLGLYVCRLLAEAQG
                     GYIWAENHPQGGAIFSLKLPLERQVRLR"
     gene            3646..4323
                     /locus_tag="AC16CB_12920"
     CDS             3646..4323
                     /locus_tag="AC16CB_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3343414.1"
                     /translation="MSDLILLIDDDHTLLELLSDHLQSMGYQVLIASDGPAGLRLATE
                     ARPDLVILDVMMPGMDGWEVCARLRENSDIPIIMLTAKGEEFDKLRGFRLGIDDYVTK
                     PFSFAELVARVGAVLTRLRQAPQTSQLISSGDLTIELAQRRVTIAGIRIELTPTEYRL
                     LEILARYANRTVPVETLLQEVWGEEYAGEVEHVKHYVWALRKKIESDPGNPQHILTDR
                     GYGYRFE"
     gene            4489..6447
                     /locus_tag="AC16CB_12925"
     CDS             4489..6447
                     /locus_tag="AC16CB_12925"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3343415.1"
                     /translation="MSNYRVFLPDLSFYQQTISCQNACPVRTDGRAYVTAIANGEYER
                     AYLIARETNPFASTCGWVCGAPCEAACRRGKIDTPIAIRALKRFVNDRYGVYLGESGE
                     AHQPPAWPAYVGPTNAFDLGNTTFPTSRSGLVAARRRSSETGKRVAIVGAGPAGLSCA
                     HDLALLGHEVTVFDSAPVAGGMLLLGVPEYRLPRELVDLEIQAILALGPSLELNQALG
                     RDFTLVDLRRSYDAVLIAIGNYRSRNLNIEGEQLDGVLRAVDFLLNVNLGGYNLDLGK
                     RVLVIGGGNVAMDVARTAARLGEPAESGGDLETALDVARAARRLGATQEVLCLVVENR
                     DEMLADPIEIVEAEKEGIQIHTHLAPKRILGGGGRAISVETLDVERAFDESGRFNPQL
                     LPGTERLWECDSVIISIGQIGDLEWIGPEDGLQTTSRGTLVIDRKTLMTTAPGVFAAG
                     DIAFGPRLIINAVADGQRAARGIHAYLQNVRPRLKRKGFFTPLSKREYPDIGPLRNYL
                     RWNRQEPPTLPVDRRIGVAHVEIGYDETKARQQGGRCLICSINPIFDGELCISCNGCV
                     DVCPTDCLKLVPISALGGDEQIATLVQGEASGWKVASAMLFDPTRCIRCGLCAARCPT
                     EAVRMEAFRFTEELWFETVDPHDASLPS"
     gene            6593..>8106
                     /locus_tag="AC16CB_12930"
     CDS             6593..>8106
                     /locus_tag="AC16CB_12930"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3343416.1"
                     /translation="MKKHRMHFSIISVLLFILAGIMVGCASQPQTSPPEVTQPPVVEE
                     TVDTHDTTSSQEDEHMEIEGDLIRGGLLYDKWWAAREEGEEGEEHVEGDEHGHEAEGP
                     EGDHPLWKTQTTNTRTGEDTWRCKECHGWDYKGIDGAYGSGSHMTGFAGIYQAKDKST
                     EELLDIMKGSTNPDHDFSSVMDEHSLNDLVIFMSQALIDTDQFVNADKSAKGDATQGE
                     TKYKDVCVYCHGPQGNAINFDSLEEPEFLGHLAQDNPWEFLHKVRFGQPGWPMPSAIT
                     NEWTDQEVTDVLAYAQTFTTDSAVSGGGLLYDKWWDVLGMEAPTTDQPLWKTQTTNTR
                     TGSDTWRCKECHGWDYKGVDGAYASGSHMTGFAGILGAATKSSDELIGWLTGKNNPDH
                     DFSGVMDETALNAMVTFMQEEMQDVSPYIDADKMTTGDPASGKQKFEKTCAGCHGVDG
                     KEMNFGDDAEPEYVGTIGVDNPWEFFHKVSYGHPGVPMPAGIGLGYTMEDRADLL"
BASE COUNT         1899 a         1938 c         2306 g         1963 t
ORIGIN      
        1 cgatattagg tgtcacggct atcctcgttg gtttagaagg gatcatcaag cggcgaattg
       61 ttttgggttc tcgttatcac cgctatggcg ttgagaccta tctcggactg gctgccactg
      121 cccaagggat gctttttctg tttttgggcg cattctttgt ggctgttgct gtggttgcct
      181 atctcactac aggtcgttcc ctcttcctct actttgtacg caaccccgga ttgcccttgc
      241 tcatctttgg ggcgggttgc ttcttggcag ctactgtagc catgcttggc tcggtcgagc
      301 aaaagcaagg cgccaaatgg atcatccttt tagatttgct cacttcacgc ctcctgccag
      361 gcctgatctt gatcatgctt ggattagccg ccacccttct gggcttgttc gaaattgttg
      421 cgccgcaggc ttttgaccag atcggcggtg gttttctgga ggtcctattc agtgcgcgct
      481 gaaaccataa ggagttggtc ggaatatcga agatctgacc gctcttaggt cagccaaacc
      541 aacgcgcaat ctcagtcttg tgatatgatg gcacagtaca gaaatacact gctgtatcat
      601 caaattacct ggggctgcgc tcgggttgag cgcatacctg gtcccgtctc accagtaaag
      661 gagtaaagca tgaagtacga tatggtattc gaaggcggcg gtgccaaagg gatggtcttt
      721 gtcggtgcaa tggaagtgtt taaatctgct gggcacaccc acgggcggct gctcggcact
      781 tcggcgggag ccatcacggc ctcattgtta gccgcaggtt accagccggg tgaaatgctg
      841 gagagcctgt ctgaaaagat cgaggggcgc tccgtatttt cagatttcat ggcggtgccc
      901 tcggctccag acgaagccac tttgcgcgcc agcgcaaccc gtgagttgtt acgtaatatc
      961 gacctgcctt ttatccccga gcgttttgag gagatactgg acgatcagct cgtcaaatgg
     1021 ttggcatctc agccgaaact gcgctccgtt ttttcattca tcgagcgcgg cgggtggtat
     1081 gcggcggatc attttgtcac ctggcttaaa cggcgcttgg atagcggcag ccatgagggc
     1141 aagccccgcc aatacagcga gatgaccctg tcggagtttt accaggcgac ccagacggac
     1201 ctgtcgctgg tggcctcgga caccacacag ggggccatgc tggtgctcaa tcaccgcacc
     1261 gcgcccgatc taccgctggt ccgggcggtg cgcatgtcca tgagcgtccc gctgctgtgg
     1321 caagaggtcg tctggcaaaa agggtggggg caatctcgcg atcgcgacat ggtcgatcac
     1381 gccatcgtgg atggcggatt gctatccaac ttccccattg agctgtttgt ctcacgcgcc
     1441 gcgtcagtga cgaatgtgat gggcgagaat gtcagccaga atgtgcttgg gatgttgatc
     1501 gacgagtcgc tggaggtgcc cggtacagaa gcggctgcca agaccccgga gaagttaagc
     1561 ctgggcgaac tgcgcactgt gcagcgcata tcgaacctgg tgaacactac cttgagcgcc
     1621 cgcgataaaa acgtcatcga tgcatttgat aagctggtgg tgcgcttgcc cgccaaaggc
     1681 tatggcacaa cggaattcga tatgaccgac gagcgccggg agttgctggt caacgccggg
     1741 cggacagcca tggaggccta ttttgaacgc cccgtccctg ttgagctcga gagtttcgac
     1801 atcggagcca caggagacga tctgcgtgcg cagcagacgg cagataagac ggcgttgaaa
     1861 attctggagt gagagggtgc tgccattaac ctgccgcgcc acagggcgcg gcaggttaaa
     1921 gaaacagaca ctaactaaaa tttgggggat catacattgg catatcgagg agttggaacc
     1981 gcccaacttg atcatggttg ttggcagggg gtgttataat actgtggctg gggtggcttt
     2041 taggaaaagc cactcttttt cttaaccgtt ctgacgatta gtaccccccc aactgaataa
     2101 ctcctgtact ttatgatgac gggagtttca tcgcaattat attatgatca atttacgaac
     2161 agaacctgcc cagcaagatg cgagtgtgaa ttatgcgtaa cgaaaaggtc agcctcagtc
     2221 tacgggttac atatttacaa aaagttttcc cgctcttttt aatctcgctt gtcatcatat
     2281 accagcttgg cctggctcgc tgggtgcatg atcactacag ccattcactc cattattggg
     2341 tcgaaatctt gttttacgct tcagtgggtc ccgtactgag tttctggata cttggactga
     2401 tcaggcggtg gctagatgag aaagagcgag cacagcaaat ggcgcgcacc agcgaacgct
     2461 atctggcatc tataaccagg gcatctgcag atgcaatttt cagcctggac cccatggggc
     2521 ggatcgaatc ctggaaccgg ggagcagaac tgatttttgg ttatgaggca ggggaaatct
     2581 tggggcggcc gctttccgat ctgtttggtg ggacggatgc tgctgtggtg gagttgcgct
     2641 ggctggtgga gagcgtagtc gaagctggtt ttgttcgcgg tcatgaaact acctgccagg
     2701 atgcggcggg tcacaaagtg atcgctgaac taacagccac acgcctggct gacgaatccg
     2761 gtcagattct cggtctttcc attatcttga gagatataac gcaacgaaaa caaagggagg
     2821 aagaaattca tcggctaaat gccaacctga tggagctggt ggctttgaga accagtgagc
     2881 tagcagagaa ggttgatcag ctggctcaag caaacgccga gttgaagcaa cttgaccggg
     2941 tgcgttcgga gtttgtctca ctggtctcgc accaattgcg agcacccctg accaacatcc
     3001 gtggagctgt ggagcgaata gaagatagct gtaaaatgag taacggaaca tgcatgcgta
     3061 tgctgaccat aatcgatcag cagatcaacc gattggacaa attggtgaag gatgtgctca
     3121 acgcaactcg aatcgagagt ggcgacttga tcatacacgc tgaaccggtc tctgtatggc
     3181 cagttgtagc ccagctagtg gagcaaatgc gcagccgcgc ttccgggcgc ccactccgat
     3241 tgtctgaaaa gcccgggctc ccgttggtct atgctgatcg ggatcgcatg gcagaggttg
     3301 tgatgaatct gcttgacaac gccgacaaat attcgccacc gggcaaagag atcctggtcg
     3361 acttgtctgc tgacgatagc ggggtgaccg tctcggtacg agactttggc gcaggtattc
     3421 ctgaaggtga tttcgagcga gtatttgaaa agttttaccg tgccgataac agcgactcgc
     3481 agtctgtata tggctacggg ttaggtcttt acgtctgccg gctgttggca gaagcacagg
     3541 gagggtatat ttgggcggaa aatcatccac agggaggtgc aatcttctcc ttgaaattac
     3601 cgcttgaaag acaggttaga cttcgataat cgcaggaagg gttatatgtc ggacctcatt
     3661 ctcctgattg atgatgatca cacgttactc gaattgcttt ctgatcatct gcaatcaatg
     3721 ggatatcaag tattaattgc tagcgatggc ccggctggtc tgcgtttggc cactgaagcc
     3781 agaccggact tagtgatttt ggatgtcatg atgccgggca tggatggatg ggaggtctgt
     3841 gctcgtctgc gtgagaacag tgatatacca atcatcatgc tcaccgccaa aggagaggaa
     3901 tttgataagc tgcgcggctt tcgcctgggg atagacgatt atgtgaccaa gccgttttcc
     3961 ttcgccgagc tggtagcgcg tgtaggcgct gttctgacaa ggctgcgcca agcacctcag
     4021 actagtcagt taatatctag cggcgacttg accatcgagt tagctcaacg gcgggtgacc
     4081 atcgcaggaa tacgaattga gctaacccca accgaatatc gtttattgga aatcctagct
     4141 cgctatgcca atcgcactgt cccggtggag accctgctcc aagaagtttg gggtgaagaa
     4201 tatgccggtg aggttgagca tgtcaaacat tacgtttggg cgttgcgaaa aaaaatcgaa
     4261 tctgatccgg gaaatccaca acatatcctg actgatcgag gctatggtta tcgatttgaa
     4321 tagaaggtac cttaaagcac tccctaattt ctttttatag atgagtttta attatttcaa
     4381 agccatcatg gtctcctagg ggacgcttgt accagtattt cacacaaatc tcccaccaat
     4441 cctagaccag atttagacct agacctgcta cacatattgc agggatagat gtctaattat
     4501 cgtgtctttc tcccagattt gtctttttac cagcagacga tctcctgtca gaacgcctgt
     4561 cctgtacgca cggatgggcg tgcctatgtg acagcaattg cgaatggcga atacgagcgc
     4621 gcatatctca tcgcacgcga gaccaatcct tttgcatcta catgcggctg ggtgtgtggt
     4681 gcaccttgtg aagctgcttg ccgtcgggga aagatcgata ccccgattgc catcagagct
     4741 ttgaagcgtt tcgttaatga ccgttacggt gtctatttgg gcgaaagcgg cgaggctcat
     4801 caaccgccgg cttggccggc ttatgtcggc ccgacgaatg cttttgattt gggcaataca
     4861 acattcccta ccagccggag cggactggta gctgcccgcc ggcgtagctc cgagacaggg
     4921 aagcgagtcg ccatcgttgg agctggaccg gcgggtctgt cctgtgcgca cgatctggca
     4981 ttgcttggtc atgaggtaac tgtttttgat tcagctcccg tcgcaggtgg gatgttgctc
     5041 ttgggagtgc ctgaataccg cttgcctcgc gagctggttg atttggagat ccaggccata
     5101 cttgccctgg gtccgtccct ggaactcaac caggcattgg gtcgagattt cacgctggtt
     5161 gatttacgac gctcctatga tgccgttcta atagccattg gcaattaccg tagcagaaat
     5221 ttgaatatcg agggtgaaca gcttgatggg gtgttgcggg cggtagattt cctgttgaat
     5281 gtcaacctag ggggatacaa cctggatttg gggaagcgtg tgctggtaat cgggggtggc
     5341 aatgtggcga tggatgtggc acgtacagcc gcacgtctcg gggaaccagc cgaatctggt
     5401 ggtgacctgg agaccgccct agatgttgcc cgtgctgccc ggcgcctagg agctacccag
     5461 gaggtgctct gtctggtagt ggaaaaccgg gatgagatgc tcgccgatcc aatcgagatc
     5521 gttgaggcgg agaaagaagg tatccaaatt cacactcacc tggctcccaa gcgcattttg
     5581 ggagggggag gccgcgcaat aagtgtggag acgctggatg tcgagcgcgc tttcgatgag
     5641 tctgggcgct tcaatccaca attgcttcca ggcacagagc gtctgtggga gtgcgatagc
     5701 gtgatcatct caattgggca gatcggtgat ctggaatgga ttgggccgga agacggcttg
     5761 caaaccacat cgcgcggcac cctggtgata gatcgaaaaa ccttgatgac cacagcccct
     5821 ggtgtcttcg cagcgggcga cattgctttc ggacctcgcc tgattatcaa cgccgttgcc
     5881 gatggacagc gtgccgcccg tggtatccat gcatatctac agaatgtacg tccgcgcctc
     5941 aagcgtaagg gcttttttac tcccctttca aaaagagaat atcccgacat aggaccactg
     6001 cgaaattact tgcggtggaa tcgtcaggag ccacctaccc ttccagtaga tcgacgtatc
     6061 ggggtggcac atgttgagat tggatatgac gaaacaaagg cccgccaaca aggtggacgc
     6121 tgcctgatct gttcgatcaa cccaatattc gatggtgagt tgtgtatctc gtgcaatggt
     6181 tgtgtagatg tctgtcccac cgattgcctg aagctggtgc caatatctgc ccttggcggt
     6241 gacgagcaaa tagcaacctt ggtccagggt gaagcgtctg gttggaaggt agccagcgct
     6301 atgctgtttg atccaacgcg ctgtatccgt tgcggtctgt gtgcagctcg ctgtccaacg
     6361 gaagcagtgc ggatggaggc gtttcgcttt acagaggaat tgtggtttga aacggtagat
     6421 ccccatgatg ccagcctgcc cagctgagaa aggagaggct ttgactatct atacacccca
     6481 atcgatcact ttatgaggat gtacttccgc ttcatctaaa acaaccgttt cgagtaaatt
     6541 tttttatatc gataagctat tttgagtgag atcaataagg agaatgccag aaatgaagaa
     6601 acacaggatg catttttcga taatcagcgt tttattattt atcctggcgg gtattatggt
     6661 gggctgtgcc tcacaacccc aaacgtctcc tccagaggta acccaaccac cagtggtcga
     6721 ggaaactgtg gatacccatg atacaacctc ctcacaagaa gatgagcaca tggaaattga
     6781 aggcgatctg atacgaggag ggttgcttta cgataagtgg tgggcggcgc gtgaagaagg
     6841 tgaggaaggc gaagaacatg tagaaggcga tgaacacgga cacgaagctg agggtcctga
     6901 gggggatcat cccttatgga agacacagac taccaatacc cgcactggtg aagatacctg
     6961 gcgttgcaaa gaatgccatg gctgggacta taagggtata gatggtgctt atggttcagg
     7021 ttctcatatg actggctttg caggtatcta tcaagccaag gacaaatcta ccgaagagtt
     7081 gctcgatatt atgaagggca gcaccaatcc cgatcatgac ttttcgtcgg tgatggacga
     7141 acatagtttg aacgatttgg ttatatttat gtcccaagcc ttgattgaca ccgatcagtt
     7201 tgtgaatgcc gacaaaagtg ccaaaggtga tgcaacccag ggcgagacca agtacaagga
     7261 tgtgtgcgtg tactgccacg gaccgcaagg taatgccatc aacttcgata gcctagagga
     7321 gcccgaattt ctgggtcatc ttgctcaaga taacccctgg gagttcctgc acaaggtccg
     7381 ttttgggcaa ccgggttggc ctatgccatc tgccatcaca aacgagtgga cagatcaaga
     7441 agtcactgat gtgcttgctt atgcccaaac tttcactaca gactcggctg ttagtggggg
     7501 agggttactt tacgacaagt ggtgggatgt tctgggcatg gaagcgccca ctaccgatca
     7561 gcccctttgg aagacgcaga ctaccaacac ccgaactggt tccgatacat ggcgctgcaa
     7621 ggaatgccat ggttgggatt ataaaggcgt ggatggagct tatgcttctg gttcgcacat
     7681 gaccggcttc gcgggaatct tgggtgcagc tacaaaatcc tcggatgaat tgatcggttg
     7741 gttgacaggc aagaacaatc cagaccacga cttctctggg gtgatggacg agacagcgct
     7801 gaatgcaatg gtgacattca tgcaggaaga gatgcaggat gttagtccct atattgacgc
     7861 cgataagatg accacagggg atccagccag cgggaagcag aaatttgaga agacctgcgc
     7921 tggatgccat ggggtggatg gcaaggaaat gaatttcggt gacgacgcag aacccgaata
     7981 cgtgggcaca attggtgtcg ataatccatg ggaattcttc cacaaagtat catacgggca
     8041 tccgggtgtg cccatgccgg ctggaattgg gctcggatac accatggaag accgggctga
     8101 cttact
//
LOCUS       DBNGSX010000435         3001 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1288022, whole genome shotgun sequence.
ACCESSION   DBNGSX010000435 DBNGSX010000000
VERSION     DBNGSX010000435.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1288022"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..802)
                     /locus_tag="AC16CB_12935"
     CDS             complement(212..802)
                     /locus_tag="AC16CB_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3343417.1"
                     /translation="MSPRAGLDTETVVEAAAEMVDREGLAALTMAQLAKQLGIRTPSL
                     YNHVDGLPGLHRARALRGLRALNEQLTRAAIGKMGSAAVMALADAFRGFVKDHPGLYA
                     ATVESVYLLRTSDEELHVENNRILDIVLAVLAPYGLTGEDALHAVRGLRSVVHGFATL
                     ELSGGFGMPLETDESFRRLVQMLIDSMGRHRQPDAA"
     gene            complement(780..1613)
                     /locus_tag="AC16CB_12940"
     CDS             complement(780..1613)
                     /locus_tag="AC16CB_12940"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3343418.1"
                     /translation="MGTKYLNTTNGQIAYDEVGEGPLVLCVPSMGDLRGEYRFLAPRL
                     ADAGYRVVTMDVRGHGESSVEWSDYTVAAIGADMLALIRHLNAGPALLVGTSMAAGAS
                     VWAAAEAEDLIAGLVLIGPFVRDMGPIWLGKLIITPLIGGPWGVALWGKYYSMLYPSR
                     KPEDFEAYTRALQSNLRQPGRKQALQRMLLASKRASEERLGEVSAPALVIMGSRDPDF
                     KDPGAEAKRVAEWLNGTAQMVEGAGHYPHAEMPEMIEPLIVGFARSAFVEKVDVATSG
                     A"
     gene            complement(1914..2570)
                     /locus_tag="AC16CB_12945"
     CDS             complement(1914..2570)
                     /locus_tag="AC16CB_12945"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HIR3343419.1"
                     /translation="MKKFVAVAGNIGVGKSTLVAMLCQRLDWQPFYEPVAENPYLADF
                     YRDMRAWAFHSQIFFLTHRLRTHRQLIDHPTSAIQDRSVYEDAEVFAQNLYRQGLITD
                     RDYGAYRELYQVLCDFLPPPDLVVYLRASVPTLLKRITQRDRDYERQITAVYLGQLNE
                     LYEDWTSRFSLCPVLTVPADDLDFVAHPRHLELIVRKIEEKLTGKEEVVFDADEVNGM
                     "
     gene            complement(2570..>3001)
                     /locus_tag="AC16CB_12950"
     CDS             complement(2570..>3001)
                     /locus_tag="AC16CB_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR3343420.1"
                     /translation="RQLNRELAEEQFILLGPGRWGTSNPDLGIHIAYGDIYHTRALVE
                     LAGQGIGQAPEASFGTHFFQDLVESSIYPLAIYLDDENTIFNRAFFYKTPNRLKNYLD
                     EETPWTDCVRVIEVASYRRGHHLELVMDDEEGISTAFLVAD"
BASE COUNT          696 a          896 c          740 g          669 t
ORIGIN      
        1 attattttgc gttgatccgt gtgcatctgt atgtatcagt gtgtatctgt gttcaatgtc
       61 cctctactgc ataaatctgt ggtaatctat ctatctctag tcattacccc aacccagcat
      121 cgatccgggt tgattcgtgt tattccttgg ctatattcca tcttccgcca aaactgaggc
      181 ctggtcttcg cctcattgtg ctggctcact atcacgctgc atcaggctgt ctgtgccgcc
      241 ccatgctgtc aatcagcatc tggaccaggc ggcgaaagct ctcatcggtt tccaatggca
      301 ttccaaaacc accggagagc tctaaagtcg caaagccgtg aaccacgctg cgcagcccac
      361 ggacagcgtg caaggcatct tcgccggtaa gcccataggg agccagaaca gccaggacga
      421 tatccaggat ccggttgttc tcaacatgca gttcctcgtc agaggttctt aacaaatata
      481 ccgactcgac cgttgctgcg taaagacctg ggtgatcctt gacgaacccc cggaaagcgt
      541 ccgccagcgc catcacagct gcagagccca ttttcccaat cgcagcgcgg gtcaactgct
      601 cattcagcgc tcgcaagcca cgcagcgccc gcgcccggtg cagtcctgga agcccatcaa
      661 catggttgta gagagatggc gtccgaatcc cgagctgctt cgctagctgg gccattgtca
      721 gcgcggctag cccttcgcgg tccaccatct ctgccgccgc ttcgacaact gtctccgtat
      781 caagccccgc tcgtggcgac atctaccttc tccacaaagg ccgacctggc gaagccaacg
      841 ataagcggct caatcatctc cggcatctca gcatggggat agtgacctgc accttccacc
      901 atctgtgccg tcccattcag ccattcagca actctctttg cttccgctcc cggatctttg
      961 aaatctgggt cacgtgagcc catgataacc agcgcaggcg cagacacctc tccaaggcgt
     1021 tcctctgagg ctcgcttgga ggccaagagc atgcgctgca atgcctgctt tcgtcctggc
     1081 tggcgcaaat tggattggag agcacgggtg taggcctcaa aatcctcggg cttcctgctt
     1141 ggatagagca tggaatagta ttttccccaa agcgcgactc cccaagggcc gccaatcagc
     1201 ggggtgatga ttagctttcc cagccaaatc ggtcccatat cgcgcacaaa aggcccaatc
     1261 aaaaccagac ctgcgatcag atcttcggct tctgccgcgg cccataccga tgcgccggct
     1321 gccattgagg tgccaaccag cagcgcaggt ccggcattta gatgccgtat tagggcaagc
     1381 atatcggcac caattgccgc aaccgtgtaa tcgctccact cgacactgct ctccccatgc
     1441 ccgcgcacat ccatagttac cacgcggtaa ccagcatcag ccagcctggg tgcaaggaag
     1501 cgatattcgc cgcgcagatc gcccatagag ggcacgcaca aaaccaatgg accctcgccg
     1561 acttcgtcat atgcaatctg cccattagtg gtgtttaagt attttgttcc catttgaata
     1621 cctccgttta tcttcaacca gtataaacta acagtgttag tattataact aatactatta
     1681 gtttgtcaag ggtaaatctc agattcagat tgaagatcgg gttatctcaa aaaaacagtc
     1741 atcgcgtggg ctagcaatgc tgtcacagaa gcaaaatatg agatagccac gccgccaaat
     1801 gagcggcgtg gctatctcct acatgatcac cttgcgtggt ttttacaacc tcatttgcta
     1861 caataccgta aaaaataaat acctgtgctt cccagtcggt atggtacact tccctacatc
     1921 ccattcacct catccgcatc aaagaccacc tcttccttcc cggtcagctt ttcctcgatc
     1981 tttctcacaa tcaactccaa gtggcggggg tgggccacaa aatccaggtc atccgccggc
     2041 accgtcagca cagggcacaa gctgaagcgg ctcgtccaat cctcatacaa ttcattgagc
     2101 tgacctaaat aaacagcggt aatctggcgc tcataatccc ggtcacgctg ggtgatgcgt
     2161 ttcaacaacg tgggcaccga ggcgcgcagg tacaccacca agtcgggcgg tggcaggaaa
     2221 tcgcacagca cttggtacag ctcacggtaa gcgccgtaat cgcggtcagt gatcagcccc
     2281 tggcggtaca agttttgagc aaagacctct gcatcctcgt agacgctgcg gtcctggatc
     2341 gccgaggtgg gatggtcgat caactggcgg tgagtgcgca gccggtgggt gaggaagaaa
     2401 atctgcgagt ggaaagccca ggcgcgcata tcgcggtaga aatctgccag gtaggggttc
     2461 tccgccaccg gctcgtagaa tggttgccag tccaaccgct ggcaaagcat ggcaaccagc
     2521 gtggatttac ccacaccaat attcccagcg acagcgacaa attttttcat cagtcagcta
     2581 ccagaaaagc agtggagatg ccctcttcat cgtccatcac cagctccaag tgatgtccac
     2641 gacggtacga agccacctcg attacgcgga cacaatccgt ccagggggtc tcttcgtcca
     2701 aataattttt cagccggttg ggggtcttgt agaagaaagc tcggttgaag atcgtattct
     2761 cgtcgtccag gtaaatcgcc aggggataga tgctcgactc caccaaatcc tgaaagaaat
     2821 gcgtgccgaa ggaagcctct ggtgcctggc cgatcccttg acctgccagc tccaccaggg
     2881 cgcgcgtatg gtaaatgtct ccgtaggcaa tgtggatgcc caggtcaggg ttcgaggtac
     2941 cccagcgtcc cggcccgagc aggatgaatt gctcctcagc cagttctcga tttagctggc
     3001 g
//
LOCUS       DBNGSX010000436         8380 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_41714, whole genome shotgun sequence.
ACCESSION   DBNGSX010000436 DBNGSX010000000
VERSION     DBNGSX010000436.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8380)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8380)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8380
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_41714"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /locus_tag="AC16CB_12955"
     CDS             complement(<1..1262)
                     /locus_tag="AC16CB_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR3343421.1"
                     /translation="MIHSFAALLSQEQVEQVHEASLEILERVGMLVRNERAREIFAAH
                     GAQVEEQTQIVRIPRQVVERYRVLFPPKFTFRARDPRYDRTLPDDSPLILTGSSAPNI
                     IDPVTGYERRSRSDDLARISHLINELDGYDVFSISTLADDAPKGQFSLSRYYPSIKNT
                     VKPVRANTPPAEADMMLSLAYLVAGGEDAFRERPFITHHYCPVVSPLTMDFDSTQLLI
                     YFSEKNLPSYFSVVPNAGLTSPLTMLATLAQCNAEFLGAAVLTQMIQPGKAVIYSTLP
                     TVADMRTGAYSPGAIESGILHMGCAQMARFYNIPSGGYIGLTNSKINDAQSGYETGMS
                     VVAGYLGGVDIFNMGGLLDALMAFDFAKAVIDNEIALMMKQIKRGFDFSRENFALDQI
                     AEIGPAGMFAGTEHTLERMRTTMLLAEVA"
     gene            complement(1259..2038)
                     /locus_tag="AC16CB_12960"
     CDS             complement(1259..2038)
                     /locus_tag="AC16CB_12960"
                     /inference="COORDINATES: protein motif:HMM:NF019322.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3343422.1"
                     /translation="MIAPYPTHSLAYSIPLYIQIAEGLISQVESGELAPGDQLPSERE
                     LSEKLGVNRMTLRRALRVLESQGLVVRRHGVGTFIAEPKIDRRMDSVFRFTRGMQNRG
                     YTPGTRLISLEQTFLDAATARELNLPISLPAYRIRRVRTVNQEAVLLESYTLPAPRFP
                     GLEAHDLESRSVYEVMESEYGVTIARARQSFEPVVASAFEAELLAVRVGAPLMLEKRI
                     SYDVDDRPVEYGKDRYRGDRFRFVTEATPFDLRLLSMEDKP"
     gene            2225..2623
                     /locus_tag="AC16CB_12965"
     CDS             2225..2623
                     /locus_tag="AC16CB_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3343423.1"
                     /translation="MATLPLIQYDEASAQVKAVFDDIKQARQIDDVNNFWKALANHPD
                     TLKRTWEILSQVMQPGALNGLTKELIYIAVSIANNCDYCIHSHTASAFAKGMTEEQYQ
                     ELLAVIGMAHQTNALATGMKVPIDPQYLNE"
     gene            2957..3982
                     /locus_tag="AC16CB_12970"
     CDS             2957..3982
                     /locus_tag="AC16CB_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HIR3343424.1"
                     /translation="MTTRHFIRGSLFTFIAIVLLASPVLAAKNGPGSPTGSGQVFLPN
                     PVAFLGDQSLTDNKDANYPELEPAYQMVTLTNLDGSGYLIGDWANIRSETGPPAFSET
                     NTFIYTRDDDRFEQVMAYFWITEAQKYIQSLGFGSTLRPINMESQDVRINQWGVDNSY
                     QWDKHDLLRFGKGGVDDAEDAEVILHEYGHAMQGDQNPALIYAGESGAIMEGFGDYWA
                     VTVSELIAPSGDPACVADWDSVSYTASPPHCLRRIDRDYHYPEDLNGRIHHDGQIWSQ
                     ALWDIRSALGNVTADTLILEAQFDFAADTSMPAAAQVTVDTAQRLYGKRTAKIVLGAF
                     QARGILP"
     gene            4125..4859
                     /locus_tag="AC16CB_12975"
     CDS             4125..4859
                     /locus_tag="AC16CB_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3343425.1"
                     /translation="MSSDSYPPNPHSDSTQQTGKIRVLIVDDHAVVRQGLRTFLELQD
                     EATSLPITVVGEASNGSQAVDQARLLQPDVVLLDLVMPEMDGVEATSRIIEKSPHSRV
                     IILTSFGEEDKVIPAIQAGAQGYLLKDIAPNDLVQAVREAYRGQVQLHPDIAKKLMSA
                     IAAQGAPSASQARESAAADLTERELDVLRLIAGGMSNNEIAEKLVISEKTVKTHVSNI
                     LSKLHLDDRTQAAIYALKHNLFSEDA"
     gene            4859..6043
                     /locus_tag="AC16CB_12980"
     CDS             4859..6043
                     /locus_tag="AC16CB_12980"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3343426.1"
                     /translation="MRIAGVYLIYTAVLLRALVIFADEPRRNLVILYLAIYGLLLLAE
                     PWINKGTVGQRTANRTWIMPIYLVVQSALATGLLFIQPFEDFFALLFVPLSLQAVLYF
                     GSRTGFLWIALFALLMTGGLLGDEQGRLFGLAMAFLYSSLCFLFGGYAHQVHQAEEAR
                     HKNQQMLGELQVAHRQLQEYAIQVEDLATEQERNRLARDLHDSVTQTVFSMNLTVQSA
                     HLLLDKEPGRVSGQLERLEELATSAMREIQALVSQLRPRSIADEGLPAALRRLAAERQ
                     TRDGLELILDVRDETGLPEPEAVGLYRIVQEALNNVAKHAETRQATIRLDLSEKPAYL
                     EIEDHGQGFDTQAVMSKRGHLGLTGMSERARELGWHLSIESHPGQGTRIRVEQSLPDG
                     ET"
     gene            6040..6828
                     /locus_tag="AC16CB_12985"
     CDS             6040..6828
                     /locus_tag="AC16CB_12985"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019350.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3343427.1"
                     /translation="MTYEPRASRYKGSYLIGFILIVVVAIRTILFFQGQTALTIIISL
                     LSAYSLLYALEPLLSNRLRWYRFIYFPLQTVLAVAITTLRPFTDISNLVYIPIAIQAL
                     HAFSRPVAIPLMTLYAAMLNITLILGMGWAEGLALGLLYLAVCTFLISYDLLYSRSQA
                     DQAESLALLADLQAAHQKLREYADQAEELSVARERNRLARELHDSVSQVIFSITLTSQ
                     AARLLLDRNPARVPEQLNSLQEMTSNALQQLRSLITQLRPQQKS"
     gene            7095..7499
                     /locus_tag="AC16CB_12990"
     CDS             7095..7499
                     /locus_tag="AC16CB_12990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3343428.1"
                     /translation="MTTSTTIKRTKTATQVLIVDDAPLVRRELRTLLSLTGELDIVGE
                     ATNGLEAIQQIEELQPDVVLMDLEMPVMDGYEATRQIKAQHPACRVVALTVHSYDEAH
                     KKALQAGVDAFIVKGTPLEKTVQSITERRIKS"
     gene            7496..>8380
                     /locus_tag="AC16CB_12995"
     CDS             7496..>8380
                     /locus_tag="AC16CB_12995"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR3343429.1"
                     /translation="MNKKSNQRRYDLDWLRVFAILFVFIYHSSRFFNLEDWHVKNIST
                     YFGVEVWNTFASSWLMPLIFVISGASLFYAMGKVSIGTIGKFIKDKVLRLFVPLMVGA
                     FTHASLQVYLERLTHGQFTGSYFEFIPHYFEGIYGFGGNFNIVGMHLWYLGVLFVFSI
                     VFLPLFLGLKSKIGARLLGRFTDFLAVPGLIYLFAMLILLSWKLIDPDSLLGFDKFGW
                     NLGVYISFFLSGFVFFSSEKLQHSIQKLRWLSLVGLITFTVLLILGQSHRDLIAWFFI
                     LAFLGFGTKQLNISTPLLK"
BASE COUNT         2022 a         2288 c         2189 g         1881 t
ORIGIN      
        1 gcaacctctg ccaggagcat ggtggtgcgc atgcgttcca gcgtatgctc ggttccggcg
       61 aacatgcctg caggcccgat ctctgcgatc tggtccagag cgaagttctc gcggctgaaa
      121 tcgaaaccgc gcttgatttg tttcatcatc agggcgatct cgttgtcgat gacagctttg
      181 gcaaagtcga aagccatcaa ggcgtctaaa agcccaccca tattgaagat gtctacgccg
      241 cctagatagc cagccacaac cgacataccc gtctcatatc ccgactgggc atcgttaatt
      301 ttggagttcg tcaggccgat atatccgcca gaagggatat tataaaagcg cgccatctgg
      361 gcacatccca tatgcaggat gccggactca atggcgcccg gcgagtaggc tccagtgcgc
      421 atatcagcca ccgtgggcaa ggtgctgtag atgacggcct taccaggctg gatcatctgg
      481 gtgagcacag ccgcccccag aaactcagcg ttacactgcg ccaacgtagc tagcatagtc
      541 agcggtgagg tgagcccagc gttaggcacg acggagaaat agctgggcaa attcttctcg
      601 ctaaagtaga tcaaaagctg ggtggagtcg aaatccatgg tcaatggcga caccaccggg
      661 cagtagtgat gggtgatgaa gggacgctcg cggaaggcgt cttccccacc agccaccaga
      721 taagccaggc tcaacatcat atccgcctca gcagggggag tgttggcccg cacgggcttg
      781 accgtgtttt tgatagaagg ataatagcgg gaaaggctga attgaccttt aggtgcatcg
      841 tccgccagcg tagagatgga gaagacatcg taaccgtcta gctcgttaat cagatgggat
      901 atgcgcgcca ggtcatcaga gcgggaacgc cgctcatatc ccgtcaccgg gtcaatgata
      961 ttcggagccg agctgccagt gaggatcagg gggctgtcgt cggggagcgt gcgatcatag
     1021 cgcgggtcgc gagcccggaa tgtgaacttg gggggaaaca agacgcggta acgctcaaca
     1081 acctggcggg ggatgcgcac aatctgggtt tgctcttcca cttgcgcgcc gtgggcagcg
     1141 aagatctctc gggcgcgttc attgcgcacc agcatgccca ctcgctctag gatctccagc
     1201 gaggcctcat gcacttgctc cacttgttct tgagaaagca gtgccgcaaa agaatgaatc
     1261 atggtttatc ctccatcgag agtaatctca gatcaaatgg cgtcgcttcg gtgacaaagc
     1321 ggaagcgatc gccgcgatag cggtctttac cgtactcgac cggtcgatca tctacatcat
     1381 atgagatgcg cttctccagc atgagcggcg cgcccacgcg caccgccagc aattctgctt
     1441 caaaggccga ggccacgaca ggctcgaaac tctggcgggc gcgagcgata gtcacgccgt
     1501 actcgctttc catcacttcg tacacagaac ggctttccag gtcatgggct tcaaggcctg
     1561 ggaagcgagg tgccgggagc gtgtagctct ccagcaaaac cgcctcctga ttgaccgtgc
     1621 gcacccgccg gatgcgataa gctggcaaag agatcggcag gttcagctcg cgggcggtgg
     1681 ctgcgtccag gaaagtctgt tccagggaaa ttaaccgtgt accaggagta tacccgcggt
     1741 tctgcatgcc gcgcgtgaaa cggaagaccg agtccatacg ccggtctatc ttaggctcag
     1801 cgataaatgt gcccaccccg tgcctgcgca caaccagacc ctgggattcc agcacccgca
     1861 gggcgcgtcg caaagtcata cggtttaccc ctagtttctc gctcagctcg cgttcggacg
     1921 gcagctggtc tcctggggct aattcaccgg attcgacctg actgatcagc ccctcagcga
     1981 tctgaatgta caggggaatg gaataggcaa gcgaatgtgt cgggtagggt gcaatcatac
     2041 ctatctcata agtggaacag tggtatacac atcttatacc acgcattata ctatgaatga
     2101 tctcatttac caatactcat agaagttacg cagttgggga ggtgatggac ctgccttttc
     2161 acattccggc ggacaagatg caatgatatg attatcaccg catccaaata atccaggaga
     2221 taaaatggct accctacccc tgatccaata tgatgaggct tctgcccagg tgaaagctgt
     2281 cttcgacgac atcaaacaag ctcgccagat cgacgacgtc aacaacttct ggaaagcctt
     2341 agcaaaccac ccagatacat tgaaacgcac ttgggaaatc ctaagccagg tcatgcagcc
     2401 gggcgcgctg aatggtctga ctaaggaact tatctatatc gctgtttcga ttgcaaataa
     2461 ttgtgattac tgcatccatt cccacacagc ctccgcattt gcgaaaggca tgacggaaga
     2521 acagtatcaa gagctgctag cggtgatagg gatggctcac cagaccaatg ccctggcgac
     2581 cggcatgaaa gtcccgattg atccacaata tttgaatgaa taaagcctgg cagatcaggt
     2641 ctgctcggca acctggggtg ggtaaaaagc actaaacaac tcatacacca ctgcaagtag
     2701 gcgcttggat ggtaacagac gttcaggtaa aaataccgcg catgtaggtt gaatcagcat
     2761 cgcaaattat gctaatatat cagcgaaata gaatgtggga tttcacagaa gaaactcaac
     2821 caattttgag ttgaccgctt tgagcttgat atttctccgc tttagttctt ttttgaacct
     2881 gtttcactca accatttggc gcacttttga gtaagtatag tgtagaaaaa tccacccaat
     2941 tagataagga ggaaatatga ccactcgaca cttcatacgg ggctctctgt ttaccttcat
     3001 cgccatcgta ctgctcgcct cgcctgtgct ggcggctaag aatggcccgg gctcgcctac
     3061 tggcagcgga caggtgttct tgcccaatcc ggtagctttt ttgggcgacc aatccctgac
     3121 cgataataaa gacgccaact accccgaact cgagccagcc taccagatgg tcacactcac
     3181 caatctagat ggtagcggct acctgatcgg cgactgggcg aatatccgca gtgagaccgg
     3241 tccacccgct ttctcagaaa ccaacacatt tatctatacc cgcgatgacg atcgctttga
     3301 gcaagtgatg gcttattttt ggatcaccga ggcgcagaaa tatatccaga gcctagggtt
     3361 tggcagcacg ctgcgcccga tcaacatgga atcccaggac gtgcgcatca accagtgggg
     3421 ggtagataat tcctaccaat gggacaaaca cgacttgctg cgcttcggca aaggcggcgt
     3481 ggatgatgcc gaagatgccg aagtgatctt gcatgagtac gggcatgcca tgcagggaga
     3541 ccagaatccc gctttgatat acgccggcga gtcgggggcg attatggagg gattcggtga
     3601 ttactgggcg gtcacagtga gcgaattgat tgcgccatcg ggtgatccgg cttgcgtggc
     3661 tgactgggat tcagtctcgt acacggctag cccaccgcac tgcctgcgcc gcatcgaccg
     3721 agactaccac tacccagagg atctcaatgg acgaattcat cacgatgggc aaatctggtc
     3781 acaagcactg tgggacatcc gcagcgccct agggaatgtg accgcagaca cgctgatcct
     3841 tgaagcccaa ttcgacttcg cggcagatac ctcaatgcct gccgcggccc aagtcactgt
     3901 ggatacggct cagagattat acggcaagcg cacagcgaaa atcgtactgg gggcttttca
     3961 ggcgcgcggc attttgccgt agtagagtga taataaggag ggtgggtagg ctcactcacc
     4021 ctccacatcc agcccaacca aggggttatt cccaagagcc atttttactc tatccaaagg
     4081 atagagtaaa attcttttaa agacatcacc taggagagca gcccatgagc agcgattcgt
     4141 atcctccaaa tccacattca gattcaacac aacaaacagg caaaattcgc gtcctcatcg
     4201 tggatgacca tgctgtcgtg cgccaggggc tgcgcacctt cctcgagcta caggatgagg
     4261 ctacatctct cccgataaca gtggttggag aggcttcaaa cggaagccag gcagtggacc
     4321 aagccaggtt gctccaaccc gatgttgtcc tgctcgatct ggtcatgcca gaaatggatg
     4381 gagttgaagc gacgtcccgc atcatcgaaa aaagccctca ttcgcgcgtc atcatcctga
     4441 ccagcttcgg tgaggaagac aaggtgatcc ccgccattca ggcaggcgcc caaggttacc
     4501 tgctcaaaga tatcgcccca aacgacctcg tgcaagcagt gcgtgaagca taccgcggcc
     4561 aggtccagtt acatcctgat atcgcaaaga aactcatgtc tgccatcgcc gcgcaaggag
     4621 caccatccgc cagccaggcg cgagagagcg ctgcggctga tctgaccgaa cgggaacttg
     4681 acgtcctgcg cttgatcgcg ggcggaatgt ctaacaatga gatcgccgag aaactggtaa
     4741 tcagcgaaaa aaccgtcaag actcacgtca gcaacatcct cagcaagctt catctggacg
     4801 accgcactca ggctgccatc tatgccctaa agcataacct gttctcggaa gatgcctgat
     4861 gcgcatagct ggcgtttatc tgatctatac agcagttttg ttgcgcgccc tggtgatctt
     4921 tgctgacgaa cctaggcgca acctggtcat cttatacctg gcgatttatg ggctgctcct
     4981 gctggctgaa ccttggatca acaagggaac agtcgggcag agaaccgcca atcggacatg
     5041 gataatgcct atttacctgg tggttcaatc tgccctggca accggactgc tgttcatcca
     5101 accgtttgaa gatttttttg ccctgttatt cgtaccgctc agcctgcagg cagtcctcta
     5161 ttttggtagc cgcacgggtt ttctttggat cgctttattt gctttgctca tgacgggcgg
     5221 gttgctgggt gacgagcaag gtcggctctt tggactggcg atggcgttcc tttacagcag
     5281 cctctgcttt ttattcggtg gttatgccca ccaggtacat caagccgaag aggcgcgcca
     5341 caagaaccag caaatgcttg gcgagctaca agtcgctcac cgccagctcc aggaatacgc
     5401 catacaggta gaagacctgg caactgagca ggaacgcaac cgcttagcgc gcgatttaca
     5461 cgattccgtc acccagacgg tattcagcat gaacctgacc gtccaatctg cccacctgct
     5521 gctggataaa gagcctggac gtgtttctgg tcagctggaa cggttagaag agttggctac
     5581 gagtgccatg cgagagatcc aagctttggt ctcacagctg cgccccaggt cgattgccga
     5641 cgagggattg ccagctgccc tgcgcaggtt ggctgcagag cgtcagaccc gcgatgggct
     5701 ggagttaatc ctggacgtgc gcgacgagac aggcctgccc gaaccagaag cagtcggttt
     5761 atatcgcatc gttcaggagg cgctcaataa cgtcgccaag cacgccgaga cgcgccaagc
     5821 gaccatccgc ctcgacctgt ccgagaagcc cgcctatctc gaaatcgaag atcacgggca
     5881 agggttcgat acccaggcag tcatgagcaa gcgcggccac ctcggcctga ccggcatgtc
     5941 tgagcgggcg cgcgagctgg gttggcattt gtcgatcgag tctcacccag gtcagggaac
     6001 gcgcatccga gtcgagcaga gcttgccgga cggtgagaca tgacatacga accccgagcc
     6061 agcagataca aaggcagcta cctgatcggg ttcattttga tcgttgtggt cgccataagg
     6121 acgattcttt ttttccaggg acagactgct cttacaatta tcatttccct cctgtcagcc
     6181 tacagcctgc tctatgcact tgagccgctg ctatccaatc gtctgcgctg gtatcgcttt
     6241 atatactttc cccttcaaac cgtcctggca gtagcgataa ccactttgcg cccctttaca
     6301 gatatatcga atctcgtgta tattccgatc gccatacaag ccctgcatgc cttctcgcgc
     6361 ccggtagcta tccccttgat gaccctctac gcggccatgc tcaatatcac gttgatcctg
     6421 ggtatggggt gggctgaggg tttggcgctc ggtttgcttt atctggccgt ttgcaccttt
     6481 ctgatttcgt acgatttgct ctatagccgc tcgcaagctg atcaggccga aagcctggca
     6541 ttgctcgcgg atctgcaggc agcccatcaa aagctacgcg aatacgctga tcaggctgaa
     6601 gaactgtctg tggctcgcga acgcaaccgc ctggcgcgcg aactgcacga ttcggtcagc
     6661 caggtgattt tcagcatcac cctcacctcg caggcagctc gcctgctcct ggatcgaaat
     6721 ccggctcgtg ttccagaaca gctcaacagt ttgcaggaga tgaccagcaa cgccctccag
     6781 caactgcgct cgctcatcac ccagctacgc ccccaacaga agtcctgacg aagaaaagat
     6841 aatatgggtt ttccagcttg agcgggaaag cgcatcattg cccgtctctc ttcgcggata
     6901 gcgccaaacc tgagctactc ccaaagtcct aagacctggg atggagcaat gccatcccaa
     6961 ggctgaccat aaaaatcgac cccatgaccg atgacaaaac acaactctag ccagatgtat
     7021 tttgctgcaa acgcgcctat attaagatcg taatctcctg agagcaagct ttcaccaaga
     7081 aaagagaaac agtaatgact acatcaacaa ctatcaaacg aaccaagact gccacccagg
     7141 tactgatcgt agacgatgcc cctctggtgc gccgggaact gcgcactttg ttgtctctta
     7201 ctggagaact agacatcgtt ggcgaggcaa ccaacggtct agaagccatt cagcagatcg
     7261 aagagctaca gccggatgtg gttttgatgg atttggagat gccagtcatg gatggctacg
     7321 aagctactcg acagatcaaa gcccaacacc ccgcctgccg ggtagtcgcc ctgaccgtac
     7381 acagctacga tgaggcgcat aagaaagctc tccaggccgg tgtggatgct ttcatcgtga
     7441 aaggcacacc gttagaaaaa acagtacagt caattacaga aagaaggatt aagtcatgaa
     7501 taagaaatct aatcaaagaa ggtacgacct cgactggctg cgggtctttg ccattctctt
     7561 tgtcttcatc taccactcca gccgcttttt taacttggaa gactggcacg taaaaaacat
     7621 cagcacgtac ttcggggtgg aggtgtggaa cacatttgca tcaagctggt tgatgccatt
     7681 gatcttcgtc atctcaggcg ccagcctgtt ttatgccatg gggaaagtca gtatcggcac
     7741 gattggaaaa ttcatcaaag ataaggtact gcgcttattc gtgcccctga tggtgggcgc
     7801 ctttacccac gcctcactgc aggtctacct ggagcgcctg acacatgggc aatttacggg
     7861 ctcatatttc gagtttatcc cgcactattt tgaagggata tatggttttg gtggcaattt
     7921 caacatagtg ggcatgcacc tttggtatct tggggtgttg ttcgtattca gcatcgtctt
     7981 cctgccgctt ttcctggggt tgaaaagcaa gattggcgcc cgcctgctgg gcaggtttac
     8041 tgatttcctg gctgtgccag ggttgatcta cctctttgca atgctgattc tcctttcctg
     8101 gaaacttatt gacccagata gtttgctggg atttgacaaa tttggctgga acctgggggt
     8161 atatatctcc ttcttcctat ccggatttgt atttttctcg agtgaaaagc tccagcatag
     8221 tatccaaaag ttgcgctggc tctcactcgt cgggctcatc accttcacgg ttttattaat
     8281 cttaggccaa tcccataggg acttgatcgc ctggttcttt atcctggcat ttctaggctt
     8341 cgggacgaag caactgaaca tcagcacgcc attgcttaag
//
LOCUS       DBNGSX010000437         4100 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_457120, whole genome shotgun sequence.
ACCESSION   DBNGSX010000437 DBNGSX010000000
VERSION     DBNGSX010000437.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_457120"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /locus_tag="AC16CB_13000"
     CDS             complement(<1..1347)
                     /locus_tag="AC16CB_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HIR3343430.1"
                     /translation="MSTQTRFLLNESDLPKFWYNVNADMPVAPAPVLHPQTMEPVTPD
                     FLSVLFPMDLIMQEISAERYIEIPEEVREIYKLYRPTPLLRARRLEKALDTTAHIYYK
                     YEGVSPAGSHKPNTAIAQAFYNKIGGTKALTTETGAGQWGSALALACNFFDLDLEVYM
                     VKVSYHQKPYRRIMMETYGAQVYASPTDRTQYGRSMLERDPESPGSLGMAISEAVEAA
                     ATSGGVKKYSLGSVLNHVLMHQTVIGEESLKQMDLAGEYPDVVVGCVGGGSNFAGIAY
                     PFLRQNLLEGKRTRLLAVEPNAAPSLTRGLYTFDYGDTARMAPIVKMHTLGHDFVPAP
                     IHAGGLRYHGMAPSLCALYDAGYIQAKSVPQLATFEAALLFARNEGIIPAPESAHAIR
                     AAIDEALDAKDKGEKRVILFNLSGHGNFDLSAYQAYLGGQLEDYEYPTEAIRASLKK"
     misc_binding    complement(1405..1731)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1876..2968
                     /gene="prfB"
                     /locus_tag="AC16CB_13005"
     CDS             join(1876..1941,1943..2968)
                     /gene="prfB"
                     /locus_tag="AC16CB_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_158066129.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIR3343431.1"
                     /translation="MQDLIERLQTCQEKLQHLMVRLDLPQKEALLDSLQKKSEDPHLW
                     DDPENAQRVMKQMSALRDEVEGWQSLRQRLHDALDLALLEEEDLRSELETETDSLETE
                     ISRREFYAMLSGRHDRGDALLAIHAGAGGTDSQDWAEMLERMYLRWAENNSYETEILD
                     FSEGEEAGIKSVTIAVNGRYAFGYLKAEKGVHRLVRLSPFDAAHRRHTSFALVEVLPQ
                     VESDDEVEINSDDLRIDVYRSSSAGGQNVQKNATAIRITHIPTGTVVTCQNERSQSQN
                     RENAMRVLRARLLELKQREQEEQMAELRGEFKKVEWGSQIRSYVLHPYQMVKDHRTDY
                     ETGNTTAVLNGAIDNFIEAYLRSIVGNGK"
     gene            3002..>4100
                     /locus_tag="AC16CB_13010"
     CDS             3002..>4100
                     /locus_tag="AC16CB_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343432.1"
                     /translation="MTIKARSSQRILLLFFSFLSLLTLSSCVTLKDPETSQEHTSAVV
                     ATLEGGHSAGQSFISRRARLNGVFLWLNVREGANSDEGMLIVQLFHAPGEKTPQAAVT
                     IPLRDAARSNPIQINLPVQSDPPAQAYYLSLLATSGIVDVLGRDEDAYPYGNATRDGE
                     TLEGDFAFRTSYDYELSAFWGDIRTSSGHWWLIFPLAIVLLLPGWLIYAILSPINVNE
                     QSDSSDRLAIAVGLSMAFIPLLMAWTSAINLPWGRLGLLFAAGMMLALMIWQLIRHKG
                     NKLKLNWYVVILLVIFATALIVRLIMVRDLAAPAWVDSVHHSLLTRIIMEQGGYPDNY
                     APYIDIDNAGYHPGFHSLLASFQWLTNLNALD"
BASE COUNT         1033 a         1054 c         1027 g          986 t
ORIGIN      
        1 cttcttcagc gaggctcgaa ttgcctcggt aggatactcg taatcctcca gctgaccacc
       61 caggtatgcc tgataagcag aaaggtcgaa gttaccgtga cccgaaaggt taaataagat
      121 cacgcgcttc tcgcctttat ccttggcatc tagagcttca tcgatagccg cccggatggc
      181 gtgcgcgctc tccggagcag gtatgatccc ttcgtttcgt gcaaagagca aagctgcctc
      241 gaatgtagcc agttggggta ccgatttcgc ttgaatatac ccggcatcat ataaggcgca
      301 taagctggga gccataccgt ggtagcgcaa accacccgca tgaattggcg ctggaacaaa
      361 gtcatgcccc aaggtgtgca tcttgacgat tggggccatg cgggcagtat ctccgtaatc
      421 aaatgtatac aaacctctgg tcagcgaagg cgcagcattg ggctcaacag ctaataagcg
      481 ggtgcgtttt ccttcgagta agttctggcg caagaaggga taggcgatac cagcgaaatt
      541 tgatccgcct ccaacacagc cgaccaccac atcgggatat tcacctgcca gatccatctg
      601 cttaagagac tcttcgccga tgacggtctg atgcatcaag acatggttca ataccgaacc
      661 caggctgtat ttcttcactc cgcctgaagt ggcggcagcc tcaaccgctt cagagatcgc
      721 catgcccaaa gacccggggc tctctggatc tcgctccaac atggagcgcc cgtattgcgt
      781 gcgatcggtt gggctagcat atacctgcgc gccgtaagtc tccatcatga tcctgcgata
      841 cggtttctga tggtaagaga ctttcaccat ataaacttct aaatctaaat caaagaaatt
      901 gcacgccagc gccaatgccg agccccattg cccagcgcct gtttcagtcg tcaaggcttt
      961 ggtacctccg attttattgt agaaggcttg tgcgattgct gtatttggct tgtggctacc
     1021 cgctggggat actccttcgt atttatagta tatatgcgca gttgtatcta atgccttctc
     1081 caagcggcgg gcgcgcaata gaggtgttgg tcggtagagt ttataaatct cgcgcacctc
     1141 ttcgggaatt tcaatgtatc gctcagcgct tatctcttgc atgattaaat ccattgggaa
     1201 aagcacggac agaaaatcag gcgttactgg ttccattgtc tggggatgca agacaggagc
     1261 cggggctaca ggcatatcgg cgttaacgtt gtaccagaac tttggtaggt cggattcatt
     1321 gagcaaaaaa cgggtttgag tagacatggt tgcctcctta ggaaagtgag tagaaaatga
     1381 agaacagaaa acgcgcccat ccctctaagg gacgaacgcg ctatgtccgt ggtgccaccc
     1441 cggttaggca gcctttggtt gaaacaaaaa cccgtcgatg atgcgacggg gattgatcaa
     1501 ctggaggcca gcctcactcg ttacggtacg ccagtaaccg attaacatgg ctactagtta
     1561 tacctatgcc tggataacgg cggcagttcc ggcgcaggct attaagcaat atgcttttca
     1621 cctggcaact ccgaggacca ttccgcttca gcgtacagac agggctctca cccgatccct
     1681 gctctctgga ctgccgtgtt gaggcgtact attcctcttc aacgtctttt aatttacagt
     1741 tgggtggatt atatactaaa agaacttgtt gtcaaggttt tgcgtcaaca tcaaaatagc
     1801 tttcacaagt tcgaagcctg cgaacttcgc cgaaatgatg taaaataagg aaaattacga
     1861 tcggagtatt attgtatgca agacctgatt gaacgcctgc aaacctgcca ggaaaaactt
     1921 cagcacctca tggtgcgtct ttgacttacc ccaaaaagaa gccctgttag attcccttca
     1981 aaaaaaatcc gaagacccgc atctttggga tgacccagag aatgcgcagc gcgtgatgaa
     2041 acaaatgtct gcccttcgag atgaagtcga gggttggcaa tcgctgcgcc agcgactaca
     2101 cgacgcgtta gatctagccc tgctcgaaga agaagacctg agaagtgagc tcgaaacgga
     2161 aacagactca cttgaaaccg aaatctcaag gcgcgagttt tacgccatgc tttcaggcag
     2221 gcatgaccgt ggcgacgcgc tgcttgccat tcatgccggt gctggcggta ccgactcgca
     2281 agattgggca gagatgttag agcgtatgta cttgcgctgg gcagagaaca atagctacga
     2341 gactgagatt ctggatttta gcgagggaga agaagccggg atcaagagtg ttactatcgc
     2401 tgttaacggg cgctatgctt tcggttatct taaagccgag aagggggtgc accgcctggt
     2461 tcgcctctcc cccttcgatg ctgctcaccg caggcatacc tcctttgcct tggtggaggt
     2521 cctcccgcag gtggagagtg acgacgaggt ggagataaac tctgacgatt tacgaattga
     2581 cgtatatcgc tcatccagcg ctggcgggca aaatgtgcaa aaaaatgcca cagcaatccg
     2641 tattacccac attcccaccg gtaccgtagt cacctgccag aatgaacgct cccagtctca
     2701 aaaccgagaa aacgccatgc gcgtactgcg cgctcgctta ttggaattaa aacaaagaga
     2761 acaggaagag caaatggctg aactgcgcgg cgaattcaag aaagtagaat ggggcagcca
     2821 gatccgctca tacgtgcttc acccttatca gatggttaag gatcatcgca ccgattacga
     2881 aaccgggaat acgaccgctg tgctcaacgg cgccattgac aacttcatcg aagcctacct
     2941 gcgctccatc gtgggaaatg ggaaatagcg agcaggaaat ctcgtaatac agaggccagc
     3001 gttgacgatc aaagccagat cgagtcagag aattttgctt ctgtttttca gctttttgtc
     3061 cctgctcacc ctctcgagct gcgttaccct caaagaccca gaaacatctc aagagcatac
     3121 cagcgctgtc gttgctactc tagaaggcgg tcacagcgct ggtcaatcct tcatctcacg
     3181 ccgggctcgc ctgaatggtg ttttcttatg gctgaacgtt cgagagggtg ctaatagtga
     3241 cgaagggatg ctaatcgttc aattattcca cgctccaggt gaaaaaacac ctcaggcagc
     3301 cgtgacaatc cccctgcgcg acgcagcccg ctccaacccg atacagatca atctacccgt
     3361 acaatccgat ccgccggcgc aagcctatta tctatcactg ttagccactt cgggaatcgt
     3421 tgacgtgctt ggccgtgatg aggatgctta cccgtatggg aacgctaccc gagatggaga
     3481 aacgctggaa ggagatttcg cattccggac aagctatgat tatgaattga gcgctttttg
     3541 gggtgacatc cgcacgtcat ctggtcactg gtggttaata ttcccgctag caatcgtact
     3601 acttcttccc ggctggttaa tctacgccat cctttcccct ataaatgtca atgaacaatc
     3661 cgactccagt gacaggctgg caattgcagt aggcctcagc atggctttca tcccattgct
     3721 tatggcctgg acatcagcga taaatcttcc ctggggtagg ttggggctac tattcgccgc
     3781 gggcatgatg ctggctttga tgatctggca attgatacgg cataaaggta acaaactcaa
     3841 attaaattgg tacgttgtta ttcttcttgt gatcttcgca actgccttaa tagtgcggtt
     3901 aatcatggtg cgcgatctgg ctgcaccagc ctgggtggat tcggtccatc atagcctgct
     3961 cacacggatc atcatggaac aaggagggta tccggataac tacgctccct acatagatat
     4021 agataatgca ggctatcatc ctggtttcca tagcctattg gcaagttttc agtggcttac
     4081 caacctaaat gctctcgatg
//
LOCUS       DBNGSX010000438         2130 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1080405, whole genome shotgun sequence.
ACCESSION   DBNGSX010000438 DBNGSX010000000
VERSION     DBNGSX010000438.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1080405"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..566)
                     /locus_tag="AC16CB_13015"
     CDS             complement(3..566)
                     /locus_tag="AC16CB_13015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3343433.1"
                     /translation="MTTMLEALNPEQIARAQRGDSATISDLYDRYRLSVYRYLYYKVG
                     DLHAAEDLTSEVFLRMIRSLNSYRPQNSSFEAWLFQIARNLAIDHYRQKNTRGHLSLE
                     EHMIHDTDDPDQKYDRSLTSDLLLKALSRLSEVQRDVIVLRFVNGLPITQVAQTLHKS
                     EDAVKGLQRRALGSLREILTEWEVTYA"
     gene            complement(722..1168)
                     /locus_tag="AC16CB_13020"
     CDS             complement(722..1168)
                     /locus_tag="AC16CB_13020"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR3343434.1"
                     /translation="MLSSSLMDMIREKYADKTGELLIPPPVFDEMAGEFVALDLDKRT
                     LSTRFPVQTKYFNPYRAVQGGIIAAAVDNTLGPLSMLVAPPSVTRRLEMKYSRQVGVE
                     SEYISVVGRLVEQKERNLIFSAEVRDSKGNLMARARSTHWIVETPG"
     gene            complement(1312..>2130)
                     /locus_tag="AC16CB_13025"
     CDS             complement(1312..>2130)
                     /locus_tag="AC16CB_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3343435.1"
                     /translation="TAQALAPDGAQPAIVGTARLAVGGVALLALAAARRALNKGGRWP
                     LMTTLIAAACMAAYQLFFFAGVARTGVAVGTVVGIGSSPILAGIIGFLVRGERPGRKW
                     ATATALAILGCSLLITAGQSIQVDPLGLLLATGAGAAYACFTVASKYLLEDRPPETVM
                     AVVFCIGALFLLPFFFTADLAWLVQLRGLAVVLHLGLVTVAIAYTLFARGLSKVPVAT
                     AATLTLAEPLTAGILGVFFLGERLTPLATFGIFLIFAGLAIISVGRKAKGATPA"
BASE COUNT          524 a          570 c          572 g          464 t
ORIGIN      
        1 aattaggcat aggtgacctc ccattcggtc agaatctcac gcaaggatcc cagggcgcgg
       61 cgttgcaagc cttttacagc atcttcgctc ttatgcaacg tctgggcgac ctgggtgatg
      121 ggcagcccgt tgacaaagcg cagcacgatc acatcgcgct gtacttcgct caatctggac
      181 agcgccttca ggagcaggtc gctggtcagg ctgcggtcat atttttggtc gggatcatct
      241 gtatcatgta tcatatgttc ctctagactc aaatggcctc tggtgttttt ctggcgataa
      301 tgatcgattg ccaggttgcg ggctatctgg aacagccagg cctcgaaaga tgaattttga
      361 ggtcggtagc tattcagcga gcggatcatg cgtaaaaaca cttctgaggt caggtcttca
      421 gcagcgtgca gatcgcctac tttgtaatac agataacggt atacactcag gcggtagcga
      481 tcgtacaggt cgctgatagt ggcggagtcg cctcgttgtg cgcgggcgat ctgttctggg
      541 ttgagagctt caagcattgt agtcatcttt ctccgtctaa catgcttaac ggattaagtg
      601 agaaaacgag tcataaaaag ggtaagttta actagatttt aattaaacga taaataaaac
      661 agtaatttct gggagaaata tccacgtcaa accatgatta ctatccatta gctgtgccaa
      721 actagccggg tgtttccaca atccagtggg tggagcgcgc cctggccatc agatttccct
      781 tcgagtctcg cacctctgcg ctgaatatca ggttgcgttc tttttgttcg accagcctgc
      841 caaccactga aatgtattcg ctctccacac caacctgacg gctgtacttc atctctaatc
      901 gcctggttac gcttggggga gcaacgagca tgctgagagg ccccagcgta ttatccaccg
      961 cggcagcaat tatgccgcct tgcacagctc gataaggatt gaagtatttc gtttgaaccg
     1021 ggaagcgcgt cgaaagcgtg cgcttgtcca gatcaagcgc cacgaactcg cccgccatct
     1081 catcgaagac agggggcgga attaggagtt cacccgtttt gtcagcatac ttttcccgaa
     1141 tcatatccat caaggaagaa gatagcatgg gccgtcacct cagattgcga atgtgcttga
     1201 agtcaaggga tgtgcaatga aaagccccgc tcttgaccgt gtctataccg aaggtattat
     1261 atatttcagt atagcacaat tactgttaaa tttttgcaat taaaaaaggc ttcaggccgg
     1321 agtggcaccc ttcgccttac gtccaaccga gattattgcc aggccagcga aaatcaaaaa
     1381 gatgccaaag gttgccagag gagtaagccg ttctcccaga aagaacactc ctagtatgcc
     1441 agcagtgagc ggctctgcca gggtcaaggt ggcggcggtt gccacaggca ccttgctcag
     1501 cccgcgagcg aacaaagtgt aggcgattgc cacggtgacc aaccccaaat gcaagaccac
     1561 cgccaggcca cgcaactgca ccaaccaggc aagatcggca gtgaaaaaga atggaagcag
     1621 gaagagcgcg ccaatgcaaa agacaaccgc catcactgtt tcaggcggtc tgtcttccag
     1681 caggtacttg ctggcgacgg tgaagcaagc ataagctgca cctgcacctg tagcgagtag
     1741 caagccgagc gggtccacct ggatgctctg accagccgtg atgagcaggc tgcaacccaa
     1801 aattgccagg gcagtggcgg tagcccactt gcgcccgggt cgttcgccgc gcacgaggaa
     1861 gccaataatc cctgccagga taggagagct gccgatccca accacggtgc ccaccgccac
     1921 gcctgttctg gccacaccgg cgaagaaaaa gagttggtag gctgccatac aggcggccgc
     1981 aattaacgtg gtcatcaatg gccagcgccc gcccttgttt aatgcgcggc gcgcggcagc
     2041 aagtgccaat agtgcgacgc cccccacggc taagcgggcc gtgccaacaa tagctggctg
     2101 ggctccatcg ggggcgaggg cctgcgctgt
//
LOCUS       DBNGSX010000439        10633 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1496296, whole genome shotgun sequence.
ACCESSION   DBNGSX010000439 DBNGSX010000000
VERSION     DBNGSX010000439.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10633)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10633)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10633
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1496296"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="AC16CB_13030"
     CDS             <1..527
                     /locus_tag="AC16CB_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343436.1"
                     /translation="GGGASELVDWINGENVPPIGRYYWLTGQSGTANSVFQAVGDHVG
                     ETVIIPVFNIICDSDDVVNECDWHTVESPPPNNHKDTDQTGAGGGLNFHIVSFSLFYI
                     SCVNAPGVSGPECPGHKYARDNGYFSAIQGVNAQNPKTIEGFIYTGNVEGLKGDPSNP
                     GNIDTGAYVFYLTR"
     gene            573..1442
                     /gene="cpaB"
                     /locus_tag="AC16CB_13035"
     CDS             573..1442
                     /gene="cpaB"
                     /locus_tag="AC16CB_13035"
                     /inference="COORDINATES: protein motif:HMM:NF024544.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HIR3343437.1"
                     /translation="MSRRVRFGIIIALVGIGLAAIGIYTIGRIIRQSLAPLPPPAPTA
                     RPSVQAVVTTHDVPLGSVLRAGDIKILEVPVELAPQGVITDLAQVTGRITKVRLVAGE
                     LVMEHNLADPTNVNHDLGFIIGDNQVLMAFPAQDLMSTLNVLQRGDLIDILVTLSQEV
                     PTASEGVGGEVVVREGDTVSRVLTFDAFQRLEITAMVVDIVQQEQRSASPVPAGEGTP
                     QPQPTPQPSDVRLRAYLLALNPQDALLLKHLKDTGATFDFVLRAPTSNQLFELQTVTS
                     DYLIDRYELSILK"
     gene            1457..2713
                     /locus_tag="AC16CB_13040"
     CDS             1457..2713
                     /locus_tag="AC16CB_13040"
                     /inference="COORDINATES: protein motif:HMM:NF012369.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpaE/TadZ family protein"
                     /protein_id="HIR3343438.1"
                     /translation="MATKAETSRVIFIGDPGAVQQQITAALGSQTDFQLVDVLSSTER
                     LIREIHVAEPNIILIDHIIGGKPTLDLIDDISLQYPDAAVITILPDNDPAVIQQVTLA
                     GARAFLVQPFTQVNLLSTLRRVTELEARRVKTQVVKESAKGEETKPMRSIAVFSPRGG
                     VGASTVALNLAIAMHEDTGARVLLMEGKFFFGHLAVMLNIRARNTIADLLPHASSLDE
                     GLVRDVVTEHASGIGVLLAPTDLQVGQGVRPDDVYSMLVNLERFFDYIVIDVGSYLTE
                     NTVTLMDTADKIMLVTTPDLASLHDASNFIQLSRSLGYPPDKIMIILNRANMEGGVRS
                     KDIEAALHHHVFAQIPDDHANALRSLNRGIPMIMHYSRSPVVKAIKQLSKRLPQNSGD
                     EPVRAKASPATSKSQSEALLASSHLG"
     gene            2732..3706
                     /locus_tag="AC16CB_13045"
     CDS             2732..3706
                     /locus_tag="AC16CB_13045"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR3343439.1"
                     /translation="MLSPEIINLLFIAFAVGLAGIITILGMANVLAGSDDISERIETY
                     ALVPEEVERRSSGWGRSSLVRLRLRINALLSFFASEEMGVRLLSANWPITETEFILIR
                     IGSVIVGFTIGWLLVRSPISGIGFAIIAFLIPEFLLRRSIYNRRRKFESQLVDVLVLM
                     KGAVRAGVGFLQSLDVVIQDMKAPASEEFRRVKREVGLGLPLSQALGNLHARMENDDL
                     YLLITALNINTQVGGNLSTMLEAVTLTIRERIQLFAEVRALTSMQRYSGYLLTLLPFA
                     TAALVFIMNPDYMGKLFEPGPFICIPIGALILVLLGNIVIRLITRIDI"
     gene            3736..4689
                     /locus_tag="AC16CB_13050"
     CDS             3736..4689
                     /locus_tag="AC16CB_13050"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR3343440.1"
                     /translation="MIDIYILGILGLFLIVGGFFALVFSMRWFGSEELSSRLTNYVTV
                     VQAQSRQLTSTTAIRNRELTGSFTSRTLLPFFRQAGSFLGRLTPANSIEQLRRQLYIA
                     GYPLGLGPREFFGLRIAFIFFGFFLSFLVLRRGINQTNLLVGAAVFIICFIFPILWLR
                     MLVNSRQDKLRKGLPDALDMLSVCADAGLGFDQSLQRISEHWSTPIGLELGRVVAEME
                     MGSSRREALRNLADRLDISEISSFVSVILQSEQLGMSIADTLHGQAEQMRIERRFRAQ
                     ELARTLPIKMLIPLAFMIFPAILAVILGPAIPQLFGLFGNL"
     gene            4698..6446
                     /locus_tag="AC16CB_13055"
     CDS             4698..6446
                     /locus_tag="AC16CB_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR3343441.1"
                     /translation="MSAESFQSYFDDPVYQSAMKHLQSGNWEAGLTELDKLVSNYPLN
                     HQLRTLRQEMKLRAQVDEDELIDIAEATKRKRITWSMRLVVSAVLLSAIIWSVMSYSQ
                     WIGQQVALARQGMENQFQDLEQAVKFRDAQDLLQVGRLSEAKTLFEEVSAKNPDYPGV
                     SEALERTDKLIGLDARYDEALRKINLGDLNGALEVLEGIEADEPFYKDVSTRIAEIKG
                     QFFLGDIQSQAENAYQLKNWEQAASSYETLRALNPSYQPEMVEDRLFESYMNAAMDTL
                     SKDTESIKALELAETYFSKALSLRPQDPTIRFERDQARETFKDRLFIRYIDAAQAALA
                     EKADSLDALATADEYFFKALELRPDDPGIQLQRRLAQLFIEAQDDFDKNRYDEVIEKL
                     EGVITEEPDYASGTARQTLFEAYLARGDARMLGGDYEPAQTDFQRAVVLAEQGENTNL
                     RLFQAQIRIAESMGAQLLYEDAVLLYRTAIDSANLSDADLKQRPELISKLKQADGYVQ
                     IRSYRSAFRIYRDQARKILFIFPKVSHVVRAGEYLTSLANQYNTTVEAILQANNLGST
                     KKVTTGQELVIPVLEP"
     gene            6473..7834
                     /locus_tag="AC16CB_13060"
     CDS             6473..7834
                     /locus_tag="AC16CB_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TadA family conjugal transfer-associated ATPase"
                     /protein_id="HIR3343442.1"
                     /translation="MFTNRPAVNNRPSVKAQDPLQSLAALETLGGVPADVYQIRDRVQ
                     KKLLSETEGEVSIEHLPQMRQTIEALFNQVLVDENLIYTRANRAILLEWVIADILGYG
                     PLEPLLQDNSITEVMVNGFDEVYIERAGKIEKTNVKFENNGHVLRIIDRIVSPIGRRV
                     DEASPMVDARLPNGYRVNATIPPLALDGPLLTIRKFSKTPYTAQDLIANGTLTPQLVA
                     FMKACVEARVNLIISGGTGSGKTTLLNVASAFIPARERVITIEDISELQLKQEHVLRL
                     EKRPPNVEGKGEVTIRQLVINALRMRPDRIIVGEARGGEALDMLQAMNTGHDGSMTTI
                     HSNSPRDTLRRIETMVLMAGLELPLKAIREQVASAIDLVIHMERLRDGTRKTVQVAEV
                     QGMEGDSVVMQDIFIFEQIGVQNGRVMGMMKPTGIRPRFSEKFLVNNIELPADIFDTA
                     TPY"
     gene            7974..8630
                     /locus_tag="AC16CB_13065"
     CDS             7974..8630
                     /locus_tag="AC16CB_13065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3343443.1"
                     /translation="MIRVAIIEDHQILVDAIELMFRLDGEFSLVGSAPTMSAGYEMIH
                     RVAPDVLLLDINLPDGNGYDLIPKIFESSPKTHVIILTSYTDEETIIRAIDVGVSGFL
                     PKNTSLADFFETIRKANEGEVVMPPKLLIGLLKRVSRDRGVMSKNDALWERLTPREQE
                     ILSHLAEGKSGNIIAEELNIAPLTVRTHVRNLMSKLGVHSRLEAVAFGVRNGLINTIS
                     "
     gene            8869..9099
                     /locus_tag="AC16CB_13070"
     CDS             8869..9099
                     /locus_tag="AC16CB_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343444.1"
                     /translation="MHEKNVDVVLLSLNSLEQPEIELPKERIQNAKVIAFSSKGDYGL
                     KRLPGKTNWEEMRPFGLKELIDELSAGRSSGE"
     gene            complement(9827..10474)
                     /locus_tag="AC16CB_13075"
     CDS             complement(9827..10474)
                     /locus_tag="AC16CB_13075"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HIR3343445.1"
                     /translation="MVKRLIVVAGNIGVGKTSLTERIGARLGWKTAFESVSDNPYLPD
                     FYADMRTWSFHLQIFFLGHRAKQYLELASMPESAILDRSIYEDAYIFARALHHLDNLN
                     ERDYLSYRRVFDLLVSKLPPPDLLIYLRAPVSVLIKRIQRRGRAIESGISAEYLSLLD
                     SFYDDWMQNFDICPVLTIRTDDLDFVNKARHLDIVVERIQDKLTGREEVVFPSEA"
BASE COUNT         2718 a         2742 c         2579 g         2594 t
ORIGIN      
        1 gcggtggtgg cgccagcgag ctggtagatt ggattaatgg agaaaacgtc ccccctatcg
       61 gcaggtatta ctggctgacc gggcagtccg gcacagcgaa ctcggtattt caggcggtgg
      121 gcgatcatgt cggcgagact gtcatcattc ccgtgttcaa cattatttgt gacagcgacg
      181 atgtcgttaa cgaatgcgat tggcatacag tagaatcacc tccgcctaat aatcataaag
      241 acaccgacca aactggcgca ggcggagggt taaatttcca tatcgtgagt ttctccctct
      301 tctatatctc ctgtgtgaat gcgccaggtg tgtccggccc agaatgcccg gggcacaagt
      361 acgccaggga taatggttac ttttccgcca tacaaggcgt aaatgctcaa aatcccaaga
      421 ccatcgaggg atttatctat acgggtaacg tcgagggatt gaagggcgac ccgtcaaatc
      481 cgggcaacat cgatacagga gcttatgtct tttatctcac tcgctaacaa attatttgtt
      541 ttattcaggc gagataacac aggaggaata ttttgagtcg gcgcgtacgt tttggcatta
      601 tcatcgcact ggttggcata ggcctggcag ccatcggcat ctacaccatc ggccggatca
      661 tccggcaaag cctggcgccc ctaccgccgc ctgcacccac tgctcgccca tctgtacagg
      721 cagtggtcac cacacacgac gtgcccctgg gcagcgtgtt gagagccgga gatattaaaa
      781 tcttggaggt tccggtagag ctggcgcccc agggcgtaat tactgacctg gcgcaggtca
      841 ctgggcgcat caccaaggtg cgtttggtgg ctggcgaatt ggtcatggag cacaacctgg
      901 ccgacccaac caacgtgaac cacgacctgg gctttatcat cggcgataac caggtcttga
      961 tggccttccc ggctcaagat ctgatgagca cactcaatgt gctccagcgt ggcgacctga
     1021 tcgacatcct ggtcaccctc agccaggaag tacccacggc aagcgaaggc gtgggagggg
     1081 aagttgttgt gagggagggc gatacggtat ccagggtctt gactttcgac gcctttcaac
     1141 gcctggagat cacagccatg gttgtggaca tcgtacagca agagcagcgc agcgcatccc
     1201 cggttcctgc tggcgaaggc acacctcagc cccagccaac cccgcagccc tcggatgtgc
     1261 ggctacgggc atacctgctg gccctcaacc cgcaagatgc cctgctgctc aaacacctca
     1321 aggacaccgg cgccaccttc gacttcgtct tacgcgcgcc gacctcaaac cagctcttcg
     1381 agctacaaac tgttacttca gactatctca ttgaccggta tgaactgtcg atattaaagt
     1441 agtaaaggaa aaacttatgg caaccaaggc agaaacctct cgcgtaattt tcatcggcga
     1501 tcccggcgct gtccagcagc agatcacggc cgcgctgggc agtcagacgg attttcaatt
     1561 ggtggatgtg ctttcatcca ccgagcggct gatccgggaa attcacgtcg ccgagccgaa
     1621 tatcatcttg attgatcata taatcggtgg gaaacccacg ctggacctga ttgatgatat
     1681 ctcgctccaa tatcccgacg cggccgtcat cactatcttg ccggataacg accccgcagt
     1741 catccagcag gtcaccctgg ctggcgctag ggcatttctg gtccagccgt tcacccaggt
     1801 gaatctcctc agtacactgc gccgagtaac cgagttagag gccaggcgcg tcaaaacaca
     1861 agtggtgaag gagagcgcta aaggtgaaga gacaaaaccc atgcgtagca tagctgtctt
     1921 cagcccgcgt ggcggtgttg gcgcctcaac tgttgcgttg aacttggcga ttgcgatgca
     1981 cgaagatacc ggcgcacgcg tgttgctcat ggaagggaaa tttttcttcg gtcacctggc
     2041 tgtgatgctc aacattcgcg cccgcaacac catcgctgac ctgctgccac acgccagctc
     2101 tctcgatgag ggtttggtgc gcgatgtagt cacagaacac gcctccggaa ttggtgtctt
     2161 gctggcgccc actgaccttc aagttgggca gggtgtccgt ccggatgacg tttatagtat
     2221 gctggtaaat ttggagcgct tcttcgatta catcgtcatc gatgtgggca gctatctcac
     2281 cgaaaacacg gtgaccctga tggatacggc cgataagata atgctggtca ccaccccaga
     2341 cctggcttcc ctgcatgatg ccagcaactt tatccaatta agccgttcgt taggctaccc
     2401 acccgataaa ataatgatca ttctaaatcg tgccaacatg gagggtggag tgcgctcaaa
     2461 agatatcgaa gctgccttgc accaccatgt gttcgcgcaa atccccgacg atcatgctaa
     2521 cgccctacgt agcctaaacc gcggtatccc tatgatcatg cactactcgc gcagtcctgt
     2581 agttaaggcg atcaagcaac tctccaagcg actcccccaa aatagcggag atgagcctgt
     2641 ccgggcaaaa gccagcccgg ccaccagcaa aagccagagc gaagcgctgc tcgcctcatc
     2701 gcatttggga taatgataaa gaactagtct tatgttgtca ccagagatca tcaacctgct
     2761 cttcatagcg ttcgccgttg ggctcgccgg cattatcaca attttgggga tggcaaatgt
     2821 gctggccggg agtgacgata tcagtgaacg tatcgagacc tatgccctcg ttcccgagga
     2881 agtggaacgc agatcgagcg gttgggggcg ctccagctta gtccgcttgc gattgcggat
     2941 caacgccctg ctctctttct tcgcctctga ggaaatggga gtgcgcttgc taagtgcaaa
     3001 ctggccgatc actgaaacag agtttattct catccgtata gggagcgtca ttgtaggttt
     3061 tactataggt tggttgttag ttcgctcccc aatatcaggg atcggcttcg ctattatcgc
     3121 tttcctgata ccagaattct tgctgcgccg aagcatctac aatcgccggc ggaagttcga
     3181 gtcacaactt gttgatgtac tggtgttgat gaaaggagca gtacgtgccg gtgtgggatt
     3241 tttgcaatcc ctcgatgtcg tcatccaaga tatgaaggcg cctgcctccg aagaatttcg
     3301 ccgggtaaaa cgggaagttg gtttaggatt gcccctctcc caggcgctcg gcaatttgca
     3361 tgcgcgcatg gaaaacgacg atttatacct gctgatcacc gctcttaata tcaataccca
     3421 ggtgggtggc aatttgtcaa caatgcttga agcagtgacc ctcaccatcc gagagcgcat
     3481 tcaattattt gctgaggtcc gggcattgac ctctatgcaa cgatattccg gctacttgct
     3541 cacgctactg ccctttgcca cagccgcgct ggttttcata atgaatccag actacatggg
     3601 caagctattt gagcccggcc catttatatg catccctata ggtgcgctga tcctggttct
     3661 acttgggaat attgtgatcc gcttaattac ccgcatcgat atataagctg cattttctag
     3721 aattgataat taccaatgat tgatatttac atactaggaa tacttggact gttcttgatt
     3781 gtgggtggat tcttcgccct ggtatttagc atgcgatggt ttgggtcgga agaattgagt
     3841 tcccgcctca ccaattacgt caccgtagta caggctcaat cccggcagct cacctccacc
     3901 acagccatcc gcaatcgtga gcttactggg tctttcacca gccgcacctt gctgccattt
     3961 tttcgtcagg ctggctcctt tcttgggcga ttaactcctg caaattcgat cgagcaactc
     4021 agacgtcagc tatacatcgc tggatacccc ctggggcttg gcccgcgtga attttttggc
     4081 ctgcgtattg catttatttt ctttggattt tttctgtcat ttttggtgct gcggagagga
     4141 atcaaccaga caaatctact agtcggtgca gccgtcttca ttatatgttt tatcttccca
     4201 atcttgtggc tgcgcatgtt agtgaactca cgtcaggaca aattgagaaa aggccttccc
     4261 gacgctctgg atatgctcag tgtctgtgca gatgctggtc tgggatttga ccagtcgttg
     4321 cagcgaataa gtgagcactg gtccacaccg attgggctcg aattggggcg cgtggtggct
     4381 gaaatggaga tgggctcgtc gcgccgggag gctctgcgca atctcgccga ccggctggat
     4441 atctctgaaa tttctagctt tgtatccgtt atcctgcaat ccgagcagct cggcatgagc
     4501 attgccgaca cactgcatgg ccaggctgaa caaatgcgca ttgagcggcg tttccgagca
     4561 caggaattgg cacgcaccct cccgatcaaa atgctcatcc ccctcgcatt catgatcttc
     4621 ccagcaattc ttgccgttat ccttggccca gcaatacctc aattatttgg tttattcgga
     4681 aatctatagg agatttaatg tctgccgaaa gctttcaatc ctacttcgac gacccagttt
     4741 accagagtgc tatgaagcac cttcaaagtg gaaactggga agccggtctc actgagctag
     4801 acaagctggt ctcgaactac ccacttaatc accaattgcg caccttacga caggagatga
     4861 agctgcgcgc ccaggttgat gaagatgaac tgatcgatat tgccgaagct actaaacgca
     4921 aacgcatcac gtggagcatg cgcctggttg tctccgctgt gctactctct gcaataatct
     4981 ggagcgtcat gtcatactcg caatggatag gtcagcaggt agcccttgcc cgccagggta
     5041 tggaaaacca atttcaggac ttggaacaag cagtcaagtt tcgtgacgct caagatcttc
     5101 tccaggtcgg tcgtctgagc gaagcaaaaa ccttatttga agaagtttcc gcaaaaaatc
     5161 ctgattaccc aggtgtaagc gaggcccttg agcgcaccga taagttgatt ggattagatg
     5221 cacgctatga cgaagcactg agaaaaatca atttaggtga cttgaatggg gcgcttgaag
     5281 tccttgaggg cattgaagcc gatgagccat tttacaaaga tgtctcaacc cgcatcgcag
     5341 agattaaagg ccagttcttc ttgggtgaca ttcaatcgca agctgaaaat gcctaccagt
     5401 taaaaaactg ggagcaagcc gcctccagtt atgagaccct gcgagctctc aacccatcct
     5461 accaacctga aatggttgag gatcgcctct tcgagtctta catgaacgcg gcgatggaca
     5521 cgctatcaaa agataccgaa tctatcaagg cactggaact tgccgagacc tatttcagca
     5581 aagcgctttc gttgcgtcct caagacccga ccatccgctt cgaacgtgac caggcacgcg
     5641 aaacatttaa agaccgcctg tttatccgct atatcgatgc cgcccaggct gcactggccg
     5701 agaaggctga ctcgctggat gcactcgcta ctgctgatga atacttcttc aaagcgcttg
     5761 agctgcgccc agatgatcct ggaatacagt tgcaacgcag gctggcgcaa ctttttatcg
     5821 aagcacaaga cgatttcgac aaaaatcgct atgatgaggt tatagaaaag ctcgaagggg
     5881 tcatcactga agagcctgat tatgcctccg gcactgcccg gcagacccta tttgaggctt
     5941 atcttgcccg aggtgatgcc cgaatgctcg gcggtgatta cgagccagca caaacagatt
     6001 ttcaacgcgc ggttgtcctg gcagagcaag gggaaaacac aaatctacgc cttttccagg
     6061 cacaaatccg gatcgccgag tcgatgggcg cgcaattact gtatgaggac gctgttttgc
     6121 tctaccgcac ggcgatcgat agcgccaatc tgagcgatgc agatttgaag caacggcctg
     6181 aacttatctc aaagctgaag caagccgatg gctatgtcca gatacgcagc taccgatcag
     6241 ccttccggat ctatcgcgat caggcaagaa aaatcctgtt tatattccct aaggtttccc
     6301 atgtggtgcg agcaggcgaa tacctgacta gtctagccaa tcaatacaat acaactgttg
     6361 aagccatcct gcaagccaat aacctgggtt caaccaagaa agtaacaact ggccaagagc
     6421 tagtaattcc cgttcttgaa ccttaatcgg ttttagttgg agagagatat ctatgtttac
     6481 taaccgtcca gcagtcaaca accgccctag cgtaaaagca caggacccat tacagagtct
     6541 cgcggcgctc gaaacgcttg gtggcgttcc tgcagatgtt taccagattc gggaccgcgt
     6601 acagaaaaaa ttgctatcgg aaaccgaggg tgaggtatcc atcgaacacc tgccacaaat
     6661 gcgccagacc attgaagcat tgtttaacca ggtgcttgtt gatgagaacc tcatctacac
     6721 tcgtgcgaac cgggccatac tgcttgaatg ggttatcgcg gacatcttgg gttatgggcc
     6781 cttggaaccc ttgttgcaag ataactcgat cactgaggtg atggtcaatg gatttgacga
     6841 agtatacatc gaacgtgctg gaaaaatcga gaaaacgaac gtaaaattcg agaacaatgg
     6901 tcatgtatta cgtatcatcg accgcattgt ctctcccatc gggcgccgtg tggacgaggc
     6961 ctctccaatg gtcgatgcgc gtttacccaa tggctaccgt gtcaacgcta ccatcccacc
     7021 cctcgcgttg gatggcccgc tcctgactat ccgtaagttc tccaaaacac cttatactgc
     7081 ccaagacctg atcgccaatg gcaccctcac ccctcaactt gtggcattta tgaaggcttg
     7141 tgttgaagcg cgcgtgaact tgattatctc tggaggtacc ggtagcggta aaaccacgct
     7201 tctcaacgtc gcttcggcat ttatccctgc aagggagcgc gtcatcacca tcgaggatat
     7261 ttccgagctc caactcaaac aggagcacgt gcttcgtctg gaaaaacgac ctcccaacgt
     7321 ggaaggtaaa ggtgaggtga ccattcgcca gctggtgatc aacgcgctgc gtatgcgtcc
     7381 tgatcgcatc atcgtcggcg aggcgcgcgg tggagaggcg ctggacatgc tacaggcgat
     7441 gaacaccggt cacgatggct cgatgaccac catccactca aacagcccgc gggataccct
     7501 gcgccgtatc gaaaccatgg tattaatggc tggcctagaa ctgcccctta aagctattcg
     7561 cgaacaggtt gcctccgcca tcgatctggt tattcacatg gaacgcttac gcgatggaac
     7621 acgtaaaact gtacaggttg ctgaagtcca ggggatggag ggagatagcg tcgtcatgca
     7681 agatatcttc atcttcgagc agattggggt acagaatggg cgcgttatgg gaatgatgaa
     7741 accaaccggg atcagaccaa gattttccga gaaattcctc gtcaacaaca tcgaactgcc
     7801 tgccgatatt ttcgacactg ccacgccgta ttaatagctg agaattgaaa tgataatttt
     7861 ggggtctgat gggtacactc gctcaccaga ccctaatttt atttggtaac cctataagct
     7921 gtaacaagtt cattaaattg acgttaacta ctataaaaga ggtatagcac aatatgatcc
     7981 gtgtagcaat cattgaagat caccagatac tggttgacgc gattgaatta atgtttcgat
     8041 tggatggtga gttttctttg gttggttcag cgccaaccat gtctgctggt tatgaaatga
     8101 ttcatcgcgt tgcccccgat gttcttttgc ttgatattaa tctccctgat ggaaacggat
     8161 acgacttaat ccccaagatt tttgagagca gccccaagac acatgtcatt atcttgactt
     8221 cttatacaga cgaggagacc attatccggg cgatcgatgt cggtgtgagc ggcttcctgc
     8281 ctaaaaacac ctcccttgcc gactttttcg agaccattcg caaagccaat gaaggagaag
     8341 ttgtcatgcc tcccaagcta ttgattgggt tacttaagcg tgtgtcgcgt gatcgaggtg
     8401 tgatgagtaa aaatgatgct ctctgggagc ggcttacccc gcgcgagcag gagatcttat
     8461 cccacttagc ggaaggcaaa tcagggaata ttatcgccga ggaactcaat attgcacctt
     8521 tgaccgtgag gactcatgtg cgaaacctta tgtccaaact aggcgttcac tcaaggcttg
     8581 aggcagttgc gtttggtgta agaaatggtt tgatcaacac aataagctga ataatttcat
     8641 cgcttaaagt acctcatttg tatgacacaa atacctcatt caggtcttaa tcagtatgcc
     8701 ctttctttat agaataaact ctagaaaggg ctattttcga tgggtcatcc acgtgtctgg
     8761 atcaactata aaccgagaat atatactgaa atatttaagc atattttcca gtccatagga
     8821 ttagtagaaa ttgtggaatt tgagccatcg aactcaggaa ggttcaatat gcatgaaaag
     8881 aatgtcgatg tcgttctgct ctcactaaat agcctggaac aacctgaaat cgaactacct
     8941 aaagagcgta tccaaaatgc caaggtgatt gccttttcgt caaagggaga ttacggattg
     9001 aagcgattac caggcaaaac taactgggag gaaatgcgcc catttggcct aaaagagtta
     9061 attgacgaac tatcggcggg aagatcttcg ggggaataat tccgagggac atagttcgct
     9121 gtatagaatt tgcgtgggtg catacccacg caaattctat ttaactatct tccaaataaa
     9181 tcgacaatct gatcatatga ataccatatt ctaagtcttc tacgccaccc tccctggaac
     9241 tgcaagaaag cttacctaca ttatctgcac taatcatttt tctccgaatg cgaatttcgg
     9301 cgcaggttat cccccacata ggtaagatct atgagctgat cattattatt gcgtttgcca
     9361 tgttctagtc ggcgtagatc actaccgaca attggaaggc cgaataatgg tgcaagcagg
     9421 catagatcca acatagcagc cagcagggtg aacatccccg gaaagatcaa aagccaggag
     9481 agaccgccaa atcgatccta aaggataccg acgatgatca ggcatatccc aatggctatg
     9541 cgcaatattc gccctgcacg gctgcttaat aatcggaaat agaaatacat ggcggtatgt
     9601 gatacacggt tgcaagtttt gaaccaacct aacaattttg ttaggttgtc aagattactt
     9661 tcccccatct caaactcctg cttatcaaaa aaacaggagt tgtgagcacc tagacccaca
     9721 actccgtgtt tcatatatca cacttctaat caacgaacgg ggcattaaac tccggtgtta
     9781 agcaacccta tctagacgca acagcaagat gctacctact caccccctat gcttctgagg
     9841 ggaaaacgac ctcttccctt cccgtcagct tatcctgtat gcgctccaca acaatatcca
     9901 gatgccgggc cttgttgaca aaatccaggt cgtccgtgcg gatggttaat actggacata
     9961 tatcgaaatt ttgcatccaa tcgtcataaa aggaatctag taaagagaga tattctgcgg
    10021 aaatgccgct ctcgatggca cgccccctac gctggatgcg ctttatcaac accgagactg
    10081 gcgctctgag atagatcaac agatcagggg gtggcaactt ggaaaccaat aggtcaaaca
    10141 cccggcgata ggacaggtaa tcgcgttcat taagattatc caagtggtgt agcgcccggg
    10201 caaatatata agcatcctcg taaatactac gatccaggat tgccgactct ggcatgcttg
    10261 ccagctccaa gtattgtttt gcacggtgcc cgaggaaaaa tatctgtaga tggaacgacc
    10321 aggtgcgcat gtcagcatag aaatcaggca gataaggatt atctgacacc gattcgaaag
    10381 cggttttcca gcccagacgg gcaccaatgc gctccgtaag ggatgtctta ccgaccccaa
    10441 tattgccagc aacaacgatg agacgtttta ccattcaaat ctccttaagt tttttcccag
    10501 cctacgactt gcatagcaca atccatgtcg tctctattat cagtttgatc atcaattgtg
    10561 ctataaccca atgaccattc acgctcgata aacacaccca ccgaggtagc cagttgatga
    10621 ttatagcatc acc
//
LOCUS       DBNGSX010000440         4811 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1772840, whole genome shotgun sequence.
ACCESSION   DBNGSX010000440 DBNGSX010000000
VERSION     DBNGSX010000440.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1772840"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..864
                     /locus_tag="AC16CB_13080"
     CDS             40..864
                     /locus_tag="AC16CB_13080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3343446.1"
                     /translation="MLGQPVVHCLTDKGHTVRILTRSAEKTHNMFGDTVDVVEGTATN
                     RDDIRAALIGCDAVHINLTPVSELAATRHVIELAEDRLERITYVSATTLSEENRWFDR
                     VDVKLCTEELLRNSGIQHAVFCPTWVMETLHNFIRGNRAVVLLGNYPPELHFFAAADF
                     GRMVAASYDDERALGKRLFVYGPEAITLQDATERFVAACYPTVKVMRWKLWQARLVAK
                     LIRNENLAEVTELIAYLDVAGEHGDPSEANTLFGAPAIALDEWFKMPKDSRKGMPH"
     gene            898..1605
                     /locus_tag="AC16CB_13085"
     CDS             898..1605
                     /locus_tag="AC16CB_13085"
                     /inference="COORDINATES: protein motif:HMM:NF025687.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HIR3343447.1"
                     /translation="MNTHRKNAIIVGVLFIIATAFLFIGESVYAPVLTTPDYLETAYP
                     ARITAILGILLEFACVLSIPLIPVFLFPVLWKHSETLALGYIVFRLFEAVLFVLDEIN
                     KLMNISLSQHYLANQGADPANFQTAGSLFQAWNDWSFSFYVFIFAIGAMMLYAVLYRS
                     KLIPRFISGWGFISAVLIFTGVLLGMLELDLNFPGMAFELIFIVPIAVQEMVMALWLI
                     VKGFDPSVISSLSAKQA"
     gene            1646..2581
                     /locus_tag="AC16CB_13090"
     CDS             1646..2581
                     /locus_tag="AC16CB_13090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018766300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIR3343448.1"
                     /translation="MKAIVYEKYGPPEVLQLKEVAKPVPKDNELLIKVHATTVTAADW
                     RMRKPDPVAARLFNGLFRPKKFNILGFELAGEVEAVGKIVKRFKPGDQVFGNTGFGYG
                     AYAEYRCLPEEGDEKKGLIAIKPASIPYEQAAAVSFGGLAALNILRKGNIQSGQKVLI
                     NGASSSTGTYAVQLAKYWRAEVTGVCSAANFELVKSLGADKVIDYTKEDFTESRENYD
                     LIFDAVGKMISGISKSKCRKALTPDGTYLSVEMSRQDHAEDLTYLAELIEAGKIKVVI
                     DRQYPLEQVAEAHRYVEKLHKKGNVVITVAHNNKS"
     gene            2655..3653
                     /locus_tag="AC16CB_13095"
     CDS             2655..3653
                     /locus_tag="AC16CB_13095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018766300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIR3343449.1"
                     /translation="MKVIFHTKYGPPDELQLKKVVKPTPKDNEVLIKIHTTTVTTSDC
                     NARNFTFVSKLLMLPARMGQGFSKPKINILGIDLAGEIEAVGQDVKRYKKGDQVFGTP
                     GNVMGAHAEYICIPEDGVLTIKPANMTWEEAASIPNMGNSALYFMRDLGNVQAGSKVL
                     INGASGGIGTFAVQLAKYYGAEVTGVCSATNLEMVKSLGADHVIDYTKEDFTQNGQTY
                     DVIFDVVYKLSFSRCKNSLNKDGVYLPTLIELPDLIQLIWTGITGGRKVKFGDAVIKV
                     ENLIFLRELFEAGKLKPVIDRIYPLEQTPEAFRYVEKGHKKGSVVIAVAQNNQGDA"
     gene            3655..4032
                     /locus_tag="AC16CB_13100"
     CDS             3655..4032
                     /locus_tag="AC16CB_13100"
                     /inference="COORDINATES: protein motif:HMM:NF039408.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6326 family protein"
                     /protein_id="HIR3343450.1"
                     /translation="MQEVKIKLSILWIVLMLTYLLGDVLRIFSGDFQAGAIDSTQISQ
                     EMYLGMAILFVLPIVMAFLSLTLKSTANRWANIVLAIVLFVINLVGLPTYPSAYDKFL
                     IIVGLIFNFLTIWYAWKWPAQAS"
     gene            4078..>4811
                     /locus_tag="AC16CB_13105"
     CDS             4078..>4811
                     /locus_tag="AC16CB_13105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010280731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIR3343451.1"
                     /translation="MKAIIYTEYGPPEVLQLQEVDKPTPKVGEVLVKVHAASVNYIDW
                     QLLKGESFFLRLLNGLTKPKQEILGDDIAGRVEAVGKMVKRFKPGDEVFGISDFGAFA
                     EYVCADEKYLALKPANLTFEEAAAVPVAGITALLSLCKHGQIQSRQKVLINGASGGVG
                     TFAVQIAKFFGAEVTGVCSTSKLDMVRSLGADIVIDYTKEDFTKSGQRYDFILAAGGY
                     HPISDYKRALSSEGVYVCCGGTFAQY"
BASE COUNT         1298 a         1046 c         1244 g         1223 t
ORIGIN      
        1 atgatgtcaa gacgaatctt ggttctgggt gggacaggca tgttgggtca gccggtcgtg
       61 cactgcctga ccgataaagg acacacggta cggattctca cccgcagcgc cgaaaaaacc
      121 cataatatgt ttggcgacac tgtcgacgtt gtcgagggaa cagcaacgaa cagagacgat
      181 atccgggcgg cgctgatagg atgtgacgcc gtgcatatca acctgacgcc agtgtcggag
      241 ttggccgcca cacggcacgt cattgaactg gcggaggatc gacttgaacg catcacctat
      301 gtctcagcca ctacactgag cgaggaaaac cgctggtttg acagggttga cgtgaagtta
      361 tgcaccgagg aactcttgcg gaacagcggc atccagcacg cggtcttctg ccccacctgg
      421 gtgatggaga cactgcataa tttcataagg ggcaataggg cagtcgtgtt acttggtaac
      481 tacccgccag agttgcactt ctttgccgct gccgacttcg ggcggatggt agccgcttct
      541 tatgatgacg aacgggcgct cgggaagcga ctgttcgttt atggcccgga agccatcacc
      601 ctgcaagatg cgacggagcg cttcgtcgcc gcctgttatc caaccgtcaa agtgatgcgc
      661 tggaaactct ggcaggcgcg gctcgttgca aaactgattc gcaacgaaaa cctggctgaa
      721 gtcacggagt tgatcgccta cctcgacgta gctggcgagc atggcgaccc ttccgaggcc
      781 aatacgctct tcggcgcccc agccatcgcc ctcgatgagt ggttcaagat gcccaaagac
      841 agccgcaagg gcatgccgca ttgaatgggt aatagagacg aataaaagga gcagaaaatg
      901 aatacgcaca gaaaaaacgc aatcattgtt ggggtgttgt tcatcatcgc caccgctttt
      961 ctgttcatag gagaatctgt ttatgctcct gtgcttacca caccagatta cctggaaacg
     1021 gcttatcccg ctcgcatcac agccatcctt ggtatcctgc tggagtttgc ctgtgtgctt
     1081 tccatccctc tcatcccggt attcctgttc ccggtattat ggaaacacag cgaaacattg
     1141 gccctaggat acatcgtctt cagacttttc gaggctgtac tctttgtgct ggacgagatc
     1201 aacaaactga tgaacattag tttaagccag cattatctgg ccaatcaggg tgcggatcct
     1261 gccaattttc aaacggcggg cagcttattt caggcctgga acgactggtc cttttcgttc
     1321 tacgtcttca tcttcgccat cggggcaatg atgctttatg ctgtcctcta tcggtcaaaa
     1381 ctaattcctc gttttatctc tggctggggt tttatcagcg cggtattgat tttcaccggt
     1441 gtgctcctgg ggatgctgga actcgatctc aatttcccag gtatggcttt tgaactaata
     1501 tttattgtcc ccatcgccgt gcaggaaatg gtaatggcat tgtggctcat cgtcaaaggc
     1561 ttcgatccat ctgtaatctc ttctctctct gctaaacagg cttaatgata tgaacgagac
     1621 tcacctaaaa aggaaggatg cctaaatgaa agcgattgta tatgaaaaat atggacctcc
     1681 agaggttctc cagctcaaag aggtagccaa acctgtccca aaggataatg aattattgat
     1741 aaaagttcat gcgaccaccg tcacagccgc ggattggcgc atgcgcaagc ctgacccggt
     1801 tgccgctcgg ctattcaatg gtctctttag gcccaaaaag ttcaatattt taggttttga
     1861 gctggccggg gaagtcgaag ctgtcgggaa aattgtaaaa cgatttaagc caggtgatca
     1921 ggtttttgga aataccgggt ttggttatgg tgcatatgcc gagtacagat gtttacctga
     1981 agaaggggat gaaaaaaagg ggttgatagc cataaaaccg gccagtattc cctatgaaca
     2041 ggctgctgcc gtttcttttg ggggattggc agcattgaat attcttagaa aagggaacat
     2101 ccagagtggg cagaaggttc ttatcaacgg cgcttctagt agtacaggta cctatgcagt
     2161 gcaacttgcc aagtactgga gggcggaagt gaccggggtt tgcagtgccg cgaatttcga
     2221 acttgtgaaa tcactgggag ctgacaaggt tatcgattac accaaggagg atttcacaga
     2281 aagcagagag aattacgatc taatttttga tgctgttgga aaaatgatct cgggaatctc
     2341 aaaatcaaaa tgtaggaaag cattgactcc ggatgggaca tacttgagtg tagaaatgtc
     2401 acggcaagat catgctgaag atctgactta tcttgcagag cttattgagg cggggaagat
     2461 aaaggtggtc atcgatagac aatatccctt ggaacaggtg gccgaggctc accggtacgt
     2521 tgagaaattg cacaaaaagg gaaacgtcgt catcactgtg gcgcataaca acaagtcatg
     2581 acaaagcgcg acatctacca ctttgatacg ctctaggatg acgatagttg ggcttatttg
     2641 taaggaggag tctcatgaaa gtaattttcc acacaaaata cggaccaccg gatgagcttc
     2701 agctcaaaaa agtagtaaaa cctactccca aggacaatga agtcctgatc aaaatacata
     2761 cgacaacggt tacaacctca gactgtaacg ctcgcaattt tacattcgtg tcaaaattgt
     2821 tgatgctacc cgctcgaatg ggtcaaggct tcagtaaacc taaaataaat atattaggga
     2881 ttgatctagc cggggaaatc gaagccgtcg ggcaagacgt aaaacgctat aaaaaaggcg
     2941 accaggtttt tgggaccccc gggaacgtca tgggtgccca tgccgagtac atctgcatac
     3001 ccgaagatgg ggtattgacg ataaaacctg ctaacatgac atgggaggaa gccgcatcca
     3061 ttcccaacat ggggaattcc gcactatatt tcatgagaga tctggggaat gtacaagccg
     3121 gaagtaaagt cttgatcaat ggcgcttccg gtgggatagg cacttttgca gtgcagcttg
     3181 ccaaatacta cggcgcggaa gttaccggag tatgcagtgc cacaaactta gaaatggtga
     3241 aatccctggg agcagatcat gtcatcgatt acaccaaaga ggatttcacc caaaatggtc
     3301 agacctatga tgttattttt gacgtagtat ataaactctc gttttcgcgc tgtaaaaact
     3361 cgctaaataa agatggggtc tatcttccaa ctttaattga actgccagat cttattcaac
     3421 tgatatggac tgggataaca ggtggcagga aggtcaaatt cggggatgcc gtcatcaaag
     3481 ttgagaacct aattttctta agagagcttt ttgaggcggg aaagcttaag ccggtcatag
     3541 acagaatcta tccgttagaa cagacccccg aggctttccg atacgtcgaa aaagggcata
     3601 aaaagggcag tgttgtcatc gccgtggcgc agaataacca aggagatgct tgatatgcag
     3661 gaggtgaaga taaaactttc tatcttatgg atagttctca tgttaacgta tcttttgggg
     3721 gatgtgctgc ggatattcag tggcgacttt caagcgggag ccatagatag cacacaaata
     3781 tctcaggaaa tgtatttggg aatggcaatc ttatttgtgc taccgattgt tatggctttc
     3841 ctgtctctta cgttgaaatc cacagccaat cgttgggcaa acatcgtatt agccatcgtc
     3901 ctttttgtga ttaacctcgt tggattgccc acatatcctt ccgcttatga caaattcttg
     3961 attatcgtag ggctcatttt taatttcttg actatttggt atgcatggaa gtggcctgca
     4021 caagcaagtt agccctaaag atgtggcgct cgatcaaggt gaaagtgaag aaatctcatg
     4081 aaagcaatta tttacacgga atacggtcca ccggaagtgc ttcagcttca agaggtagat
     4141 aagcccactc ctaaggttgg tgaagtactg gtaaaagtcc atgcggcatc cgtaaactat
     4201 atagactggc aactcctgaa aggtgaatcg ttctttctgc ggctattaaa cgggcttacg
     4261 aagccaaaac aggagatact cggagatgac atcgcagggc gggttgaagc ggttggaaaa
     4321 atggtcaaac gttttaagcc aggtgatgaa gtgttcggaa taagcgattt tggcgcattt
     4381 gccgagtatg tctgtgctga tgaaaaatat ttagcactga aaccagccaa tttgacattc
     4441 gaggaagcag cggctgtgcc tgtagctggg atcaccgccc tgttgagtct ttgtaagcat
     4501 ggacagattc agtcccggca gaaggttttg ataaatgggg cctctggagg agtgggcacg
     4561 tttgcggtgc agattgcgaa gttttttggg gctgaagtga cgggtgtttg cagcacaagc
     4621 aaattggata tggtgcgctc gcttggggca gacattgtca tagattacac aaaagaagat
     4681 ttcaccaaaa gtgggcagcg ttatgacttt attcttgctg ctgggggata tcacccgatt
     4741 tctgattata agcgtgcatt aagctctgag ggggtttatg tatgttgcgg tggtaccttt
     4801 gctcaatatt t
//
LOCUS       DBNGSX010000441         4358 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1426761, whole genome shotgun sequence.
ACCESSION   DBNGSX010000441 DBNGSX010000000
VERSION     DBNGSX010000441.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1426761"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1350)
                     /locus_tag="AC16CB_13110"
     CDS             complement(46..1350)
                     /locus_tag="AC16CB_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HIR3343452.1"
                     /translation="MKRTSIVFILSVIIVILAVALVGTLLFIKNQPASTRGVAPLTQI
                     DEYEPDSSKWGVNFPNQYSTLKLTEINKARSTYGGSEPFSKLEDDPRLVTLFAGYGFS
                     KDYNEERGHLNSLTDVRATLRVNETTPGTCYSCKSSDNPRLWAEMGMAEFDKTLFKDL
                     GAQISNPIGCANCHEPNTMRLIVTNPALEEALQAQGKDWRMFTRQEMRTVVCANCHVE
                     YYFAGEGKYLVFPWKDGSKIENIAQFYADLEFKDWEHPQSGAPMIKMQHPEYEFFTAD
                     STHYKAGVACADCHMPYTRDGAVKFSTHDVKSPLQNADTACGACHTDVTYVTGRVAII
                     QDQVRETMDASEDALVAAIQAIEAAAAATDVSTDLLGEARRLHREAQLRWDFIAAENS
                     MGFHNPEEALRILANATDLARQAQLVAVQAAGTPSIIQTSSQ"
     gene            complement(1347..1829)
                     /gene="nrfH"
                     /locus_tag="AC16CB_13115"
     CDS             complement(1347..1829)
                     /gene="nrfH"
                     /locus_tag="AC16CB_13115"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF015237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HIR3343453.1"
                     /translation="MKLALIVGFTALVIVIGVGLYVTDFTAYLGNNPTTCNNCHVMDA
                     VYESWYHGGHKQWANCSDCHTPHALIPKYIVKAENGYHHVSAFILGRIPDAIRSREVS
                     RQIVQENCVYCHAETVSTILEGPMAFDRYCIDCHRSVAHGERGISILPYQHSEEYIER
                     "
     gene            2233..3135
                     /locus_tag="AC16CB_13120"
     CDS             2233..3135
                     /locus_tag="AC16CB_13120"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR3343454.1"
                     /translation="MAKKTALVLSGGGAKGAFQCGAEKYAREVKGYKWDIIAGVSVGA
                     LNGCMLAMEKYERLFEVWNTISNDRVYTGGFNIWSGIKLLFGAKSFYSNDPLRRMLSQ
                     ELEPDKIKTDLRVGAVSLVSGEYVEFRADSPNLVEAILASTVMPIIWTPVDVSPLYQA
                     MVDGGVRNISPIGDVLETEPDEVVIINCSPEATDMLPDPPANIGKIGLRTLDILLNEL
                     FRSDMSEFVRINHLVKEAEAEGVTLHHPRTGRPLKYYDCKIIEPLQSLGDTLDFSQDS
                     VQRSLKTGVERARQVLEGVKRFDH"
     gene            3178..>4358
                     /locus_tag="AC16CB_13125"
     CDS             3178..>4358
                     /locus_tag="AC16CB_13125"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3343455.1"
                     /translation="MARGERQSPHPERSDAEARQDISQDKLDFKKILPVFVIVLIDLL
                     GLTIIIPLMPLYAASFGGDALMIGILGGTYPAMQFVGAPLLGRLSDRYGRKPVLLISQ
                     MGTFIGFIVLGFANALWLLFLARFIDGLSGANISTAQAVISDSTTDKNRTQGLGLVGA
                     AFGLGFVIGPIIAFLSLAVSGNDYRVPAFVAAAFSAISILLTWFLLEETLPAERRGAA
                     AGKASVSIGGMLVALRRSDVGLLLGLMFAQQIAFGGFEQLLALFTLNRMGLNASGNAI
                     IFVFVGVLVVAVQGGFIGQWSRRLGDRRLIYLGLATLSVGLMLTAFTPRQPMPSYSQA
                     KLAAELSAGSGDFPTHENPTTRNLKIDLPPDSDKGWLGLTWILLAMIPVSIGGGILHP
                     G"
BASE COUNT          890 a         1119 c         1282 g         1067 t
ORIGIN      
        1 accccctaaa ttccacttgt ctgtggagat cccagaggcc ctgacttact gactactggt
       61 ctggataata ctcggcgtgc cggcagcttg aacggccact agttgagctt ggcgggcgag
      121 atctgtggcg tttgccagga tacgtaaggc ctcttcgggg ttgtggaagc ccatactgtt
      181 ctccgcagcg ataaagtccc agcgcaattg agcctctcgg tgcaggcgac gcgcctctcc
      241 caataggtct gtgctaacat ccgtagctgc tgcagctgcc tcgatcgcct gaatggcagc
      301 caccagggca tcttcactgg catccattgt ctcacgcacc tgatcctgga tgatagccac
      361 ccgcccggtc acataagtca catcggtgtg gcaagcgcca caagcggtat cggcattttg
      421 cagcgggctt tttacatcgt gtgtcgagaa cttgaccgcg ccatcgcggg tgtagggcat
      481 gtggcagtcg gcacaggcta ctccggcttt atagtgggtg ctgtcggcgg tgaagaactc
      541 gtattcgggg tgctgcatct tgatcatcgg tgcaccggac tgcgggtgtt cccaatcttt
      601 gaattccagg tctgcataga attgagctat gttttcaatc ttggagccgt ccttccaggg
      661 gaagaccagg tattttccct cgccagcgaa gtagtattcc acatggcagt tggcgcaaac
      721 tacggtgcgc atctcctggc gggtgaacat acgccaatcc ttgccctgtg cttggagggc
      781 ttcttccaaa gcgggattgg ttacgatcag gcgcatggta ttgggctcat ggcagttggc
      841 gcatccgatc gggttgctga tttgcgcccc caaatccttg aagagcgtct tgtcaaattc
      901 agccataccc atctctgccc acaggcgggg gttgtcggag ctcttgcaag agtagcaggt
      961 gcctggggtc gtctcgttga cgcgcagggt ggcgcgcaca tcggtcaggc tgttcaggtg
     1021 gccgcgctct tcattgtagt ctttgctgaa gccgtatcca gcgaaaagcg tcaccagacg
     1081 aggatcatcc tccagcttgg aaaaaggctc cgaacccccg taggtgctgc gagctttgtt
     1141 gatctcggtc agcttgaggg ttgaatactg gttggggaaa ttaacgcccc acttggatga
     1201 gtcgggctca tactcgtcga tctgcgtcag gggggcaaca ccacgagttg aggcaggttg
     1261 gtttttgata aaaagcagtg tgcctaccag ggcaactgcc aggatgacga tgatgacaga
     1321 taaaatgaaa acaatgcttg tgcgtttcat cgttctatgt actcctcaga atgttggtaa
     1381 ggcaggatcg agatgccgcg ctcgccatgt gccacgctgc gatggcaatc gatgcagtag
     1441 cggtcaaaag ccattgggcc ctcgagtatg gtggataccg tctcggcatg gcagtacacg
     1501 cagttctcct ggacgatctg gcgcgagact tctcgagaac ggattgcatc aggtatgcgc
     1561 ccaagaataa aggcggagac gtgatgatag ccgttctcgg ctttgacaat gtactttggg
     1621 attaaggcgt gcggggtatg gcagtcagaa caattagccc attgcttatg cccgccgtgg
     1681 taccagcttt cgtagacggc atccatgacg tggcagttgt tgcaggttgt tggattattg
     1741 cccaggtatg cggtgaaatc ggtgacatac agcccgactc cgatcacgat cacaagggcg
     1801 gtgaatccaa cgatgagagc aagtttcatg gcccaacctt tggtgtcagg attcattgtt
     1861 cgcgcatttg gagtaacggc ctggcgaccc aaacagcagg tctgtgatat ttgtcacaat
     1921 aatagattgt agtataccat accagtaata ccggtcgcgg cgctggtcag acgaatatca
     1981 gcctgttcgt gggtcagcca acctgttagg gcaatcgcat ttgggcgatt tcccaccact
     2041 gaggagagaa aatgggatgt tttggtgggg gtatgccccc aaacactctt tttgcgggat
     2101 tgcgcatgcc cctcagggca tatgcaattg ccctggccag cctgttttcc cgagtccaaa
     2161 ttttactttt ggatggtatg attattgcta ctccaagggg tataggtcca tgatggatgg
     2221 aaggaggcga cgatggctaa aaagacggca ctggtgctca gtggtggagg cgcaaaaggc
     2281 gccttccagt gcggcgcgga gaaatatgcc cgtgaggtga aaggctataa atgggacatc
     2341 atcgctgggg tttctgttgg agcgctgaat ggctgcatgc tggctatgga gaaatacgag
     2401 cggctatttg aggtctggaa tacgatctcc aacgatcggg tctatactgg cgggttcaat
     2461 atttggtccg gcatcaagtt gctctttggt gccaaatcat tctatagcaa tgatccgctc
     2521 cggcgcatgc tttcacagga attggagcct gataaaatta agacagacct gcgcgttggg
     2581 gcagtttccc tggtgagcgg cgagtatgtc gagtttcggg ctgactcacc caacctggtg
     2641 gaggccatcc tggcgtctac ggtcatgcct atcatctgga cgcccgtaga tgtctctccg
     2701 ctttaccaag ccatggtgga cggcggagtg cgcaatatca gccctatcgg tgacgtattg
     2761 gaaaccgaac cggatgaggt tgtaatcatc aattgcagcc cggaagcaac ggatatgctc
     2821 ccggatccac cggctaatat tggcaagatt ggcttgcgca cgctggatat cctgctcaac
     2881 gagctgttcc gcagtgatat gagcgagttc gtacgcatca accacctggt caaagaggct
     2941 gaggcggaag gcgtcaccct gcaccacccc aggacaggca ggcctctgaa atattatgat
     3001 tgcaagatca tcgaaccttt gcagtccctc ggggatacac tggatttttc tcaggactcg
     3061 gtacagcgct cgctgaagac aggtgtggag cgcgcccggc aagtattaga aggtgtcaag
     3121 agattcgatc attgatggtt ttattcggta tcatctcctt cctatggagg gagcatagtg
     3181 gctaggggag aaaggcaaag cccacatcca gagcgctcgg acgccgaagc gaggcaggat
     3241 atttcacaag acaaactgga tttcaagaag atcctgcctg tatttgtgat cgtcctgatc
     3301 gacctgcttg ggttgaccat catcatcccg ctgatgcccc tttatgcggc gtctttcgga
     3361 ggggatgcgc tgatgatcgg cattcttggc gggacgtacc cggcaatgca gtttgtgggc
     3421 gctccgctcc tagggcgttt atccgatcgc tacggtcgca agccggtgtt gctcatcagc
     3481 cagatgggca ccttcattgg cttcatcgtc ctgggctttg ccaatgctct ctggttgctc
     3541 ttcctggcgc ggttcatcga cggtctatca ggcgccaata tctccactgc ccaggcggta
     3601 atttccgata gcaccacaga taagaaccgc acccaagggt tggggttagt tggagcggct
     3661 tttgggctgg gattcgtgat cggcccgatc atcgcttttc tctccctggc agtgagcggt
     3721 aatgattacc gcgtcccggc gtttgtggct gccgcttttt cggcgatctc tatcctgctg
     3781 acctggtttt tactggagga gacgctcccg gcggagcgac gcggcgctgc tgctgggaag
     3841 gcctccgtct cgatcggggg gatgctggtg gccttgcgcc gctcggatgt gggcttgctg
     3901 ctcgggctca tgtttgccca gcagatcgcc ttcggtggct ttgaacagtt gctggcactg
     3961 tttaccctca atcgcatggg actgaacgcc tccggtaatg ccattatttt cgtattcgtg
     4021 ggtgtgctgg tagtggcggt acagggtgga tttatcgggc aatggagccg cagactgggg
     4081 gatcgcaggt tgatctatct gggattggct accctttccg ttgggctcat gctgactgcc
     4141 ttcacgcccc gccagcccat gcctagctac tcccaggcca agctggctgc ggagctcagc
     4201 gcgggtagtg gggatttccc cacgcacgaa aatccaacca cgcgcaacct gaagattgat
     4261 ctgccaccag acagtgataa aggttggctt gggttgacct ggatcctgct tgccatgatc
     4321 ccggtttcca tcggtggtgg aatcctccat cccggcat
//
LOCUS       DBNGSX010000442         3493 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1842134, whole genome shotgun sequence.
ACCESSION   DBNGSX010000442 DBNGSX010000000
VERSION     DBNGSX010000442.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1842134"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="AC16CB_13130"
     CDS             complement(<1..710)
                     /locus_tag="AC16CB_13130"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017316620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR3343456.1"
                     /translation="MQKILAQAGLGSRRSCEEIIAAGRVRVNGQVALLGTKADPQRDD
                     IIVDGKPIKVDQSMVYIALYKPRGVLSTVTAPDPRSTVRDLVSVPGKLYPVGRLDVDS
                     EGLVLLTNDGELANKITHPRYGHEKEYQVLIARRPDNDQLQTWRRGVVLEDGHRTAPA
                     EVNFVRTHGKGAWLRIIMREGRKRQIREVGGRLGLPVVRIIRTRIGTLLLGNLKPREW
                     RHLTQREINDIKKSIKSSP"
     gene            complement(732..1283)
                     /gene="scpB"
                     /locus_tag="AC16CB_13135"
     CDS             complement(732..1283)
                     /gene="scpB"
                     /locus_tag="AC16CB_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR3343457.1"
                     /translation="MSAKLEALLFASPSSVTPSQLAIALEITTQEVEAGLKELELRYT
                     QENFKFGLRLQRLHGRIQLTTAPESAPLIERLLGLEATSRLSRAALEALAIIAYTEPV
                     TRPQIDAIRGVNSDGVLKSLLNKGLIQEIGRAEAPGRPILYSITQDFLGHFGLNSLDD
                     LPPLPPQDGDQENTVSTDELTAL"
     gene            1374..1946
                     /locus_tag="AC16CB_13140"
     CDS             1374..1946
                     /locus_tag="AC16CB_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343458.1"
                     /translation="MYSRNYLPVALAVVVLLLVNMACNLTQRSEPTPAEVIPITTEAV
                     ESLKKDLQKAGKEIKLSGQTTLVIDEAELTSLAAFELQNQDSQVFQDPQIFLRDGQIK
                     IATQVEQGNSRASVDIVMEVSADADGRPEYQLVSAKIGPLPLPESMSQQIYKLIDDAF
                     TSKIDPQMDDIFIDNITIKNGLMTVQGHLR"
     gene            2036..>3493
                     /locus_tag="AC16CB_13145"
     CDS             2036..>3493
                     /locus_tag="AC16CB_13145"
                     /inference="COORDINATES: protein motif:HMM:NF019142.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HIR3343459.1"
                     /translation="MSIAADGQNVELQVSAGTTVQQALEAAAITLGGLDRVEPPSYTL
                     LTDGASIRVVRVREEFTIEEEVIPFERQVLQSESLSDQQQLLVQSGVNGLNELTYRKV
                     FENGVEISNTPVKAVIVKDAIPEIIMVGIRAPFTPAEITGRIVYLLGSNAWMMEGSTA
                     NRRPLINSGDLDGRVFSLSEDGRWLLFTRRASEVDDINTLWVADLNADPVDEIDLKVA
                     NVVHFADWVPGSTSKVVFSTVEPRASPPGWQANNDLNALSFSPSGWVSNWQAKPVLET
                     NSGGVYGWWGMNFFWSPNGQRLAFSRPDSVGVLEFKDGVMTTLLDITPLQTGEDWAWV
                     PGVAWGADGNVLYTVSHEAPEDSQLFNLSAIPVESGVPIQLSQESGMFAYPAISPMQS
                     LPSGESAYKVAFLRAVLPRQSKSSSYRLVVMDRDGSNQNILFPPQGEPGLEPQQVVWS
                     PAPLPGDGIYTLAILYQGNFWLVNSITGDAQQITGD"
BASE COUNT          847 a          822 c          911 g          913 t
ORIGIN      
        1 ggcgaagatt taatgctttt cttaatgtcg tttatttccc gctgggtgag atggcgccat
       61 tctcggggtt ttaggtttcc cagtagtagt gtgccaatgc gagtgcggat gatgcgcaca
      121 acagggaggc caaggcgccc ccctacctcg cgaatttgcc gtttgcgccc ttcacgcatt
      181 atgatgcgca gccaggctcc ttttccatgt gtccgtacaa aattcacttc ggcaggcgct
      241 gtccgatgac catcctctag aaccacgccg cgccgccagg tttggagttg gtcattatca
      301 gggcgtcttg cgatgagcac ctgatattcc ttttcgtgcc catagcgtgg gtgagtgatc
      361 ttgttggcaa gctcaccatc attggtgaga agtaccaacc cctcactgtc tacatccaat
      421 ctacctactg gatagagttt gccgggaaca gacacaaggt ctcgtaccgt ggaccgaggg
      481 tctggagcgg tgacggtgga aagcacgcca cgaggcttat acagggcaat atacaccata
      541 gattggtcaa cctttattgg cttaccatcc acgataatat cgtcgcgttg tggatcggct
      601 ttagtcccca gcagagcgac ttgaccattg accctaacgc gcccagcggc tatgatctct
      661 tcgcaagacc gcctagatcc tagcccagcc tgtgccagta ttttttgcaa acgttcttcc
      721 actggagatt attataatgc agtcaactca tcagtggata ctgtattctc ctgatcgcca
      781 tcttgaggcg ggagtggtgg taggtcgtct agcgagttca agccaaaatg ccctagaaag
      841 tcctgtgtaa tactgtataa gattggcctt ccaggggctt cagcgcggcc aatttcctga
      901 attagccctt tgttcagcaa gcttttcagc acgccatcgc tatttacgcc gcggattgca
      961 tcaatttgcg ggcgcgtgac gggttccgtg taggcgataa tcgccaaggc ttccaatgca
     1021 gcccggctaa gccggctggt ggcttccagt cccaataaac gttcaataag tggagctgat
     1081 tcaggagcag ttgtaagctg gatccgaccg tgaagtcgtt gcaaacgcaa cccaaactta
     1141 aagttttcct gagtgtagcg caattccagc tcttttagac cggcttccac ttcttgggta
     1201 gttatctcca aagcaattgc cagttgtgat ggcgtcaccg aagacgggga agcaaataga
     1261 agcgcttcaa gtttagcgga caaactcagt tcattttcgg gttcaattgt ctctaccatg
     1321 ctatgataaa atgtgccgga gcgttgaaga aatagatcga aatggagaat ttaatgtatt
     1381 caagaaatta tctgccagtt gcgctggcgg ttgttgtatt attgcttgta aacatggctt
     1441 gcaacttgac tcagagatca gagcccaccc cagcagaggt tattccaatt acaaccgaag
     1501 cggttgaaag tttaaaaaag gacctccaga aagctggtaa agagatcaaa ctgagcggtc
     1561 aaactactct ggtcattgat gaagcggaat tgacctcatt ggctgctttt gaacttcaaa
     1621 atcaagattc ccaagtgttc caagacccac agatattcct tcgggatggg caaatcaaaa
     1681 ttgccacaca ggttgagcaa gggaattctc gagcaagcgt tgatattgta atggaggtat
     1741 ctgccgatgc agatggcagg ccagaatacc agttagtttc ggcaaaaatt ggtccactac
     1801 cgctgccaga gtccatgtcc caacagattt ataagctgat cgacgatgcc tttacgagta
     1861 aaattgaccc acagatggat gacatcttta ttgataatat cacaatcaag aatgggctca
     1921 tgaccgtaca aggtcatttg cgatgagatt ataccatccc aaaatcggaa ttcttctgct
     1981 ttgccttgtg ttggctgcct gttcgcctcc tcaagctact cagtcaacta ttaatgtgag
     2041 tatcgctgct gatgggcaaa atgtggagtt gcaggtttca gcaggtacca ctgtccagca
     2101 ggcgctggaa gcagcagcga ttaccctagg ggggctggat cgcgttgagc caccatctta
     2161 tacgctgttg acggatggtg catcaatacg ggtggtgaga gtgcgcgagg agttcaccat
     2221 tgaagaggaa gtgatcccgt ttgagcgcca agttttgcag agcgagtcgc tctccgacca
     2281 gcaacaattg ctggtccaga gcggggtgaa tggcttgaat gagctcactt accggaaggt
     2341 atttgagaat ggcgtagaaa tttccaatac gccggtgaaa gcagtgattg tcaaggatgc
     2401 gatcccagaa atcatcatgg tgggaatacg tgctcccttt acgccagctg aaattaccgg
     2461 gagaatagtg tatcttctcg gcagcaatgc ttggatgatg gaaggcagta cagctaaccg
     2521 gcgtccatta attaattccg gtgatttaga tggacgagta ttttcgttgt ctgaggatgg
     2581 ccgctggctt ttgttcaccc ggcgtgcctc ggaagtggat gacataaaca cgctgtgggt
     2641 ggctgacctg aatgccgatc cagtggatga gatcgacctg aaggtagcaa atgtcgttca
     2701 ttttgcagat tgggttcctg gatcaacctc gaaagtggtg ttttctactg tcgagccacg
     2761 cgccagccct cctggatggc aagccaataa tgatttaaac gccctcagtt ttagtcccag
     2821 cggctgggtt tccaattggc aggcaaaacc ggtattggaa acgaattcag gtggtgttta
     2881 tgggtggtgg ggcatgaatt ttttttggtc tccgaatggc cagcgtctcg ctttttctcg
     2941 ccccgacagt gtcggcgtgc tggaatttaa agacggagtg atgaccactc tgctggatat
     3001 cacgcccttg cagactggag aagattgggc ttgggtacca ggtgttgctt ggggagcgga
     3061 cggaaatgtt ctctacacgg ttagccatga agcgccagaa gattctcaac tttttaatct
     3121 ctccgccatt cctgtagaga gtggcgtacc gatccaacta tctcaagaat caggcatgtt
     3181 cgcttaccct gcgatttccc cgatgcaatc gctccctagt ggtgagagtg catataaagt
     3241 ggcatttttg agagcagtac tccctcgcca gagtaagtcc agttcttatc gtttggtggt
     3301 catggatcga gatggttcga accagaatat cctatttccg cctcagggtg agcctgggct
     3361 ggaaccacag caggtagtat ggtcacctgc gcctcttccg ggcgatggaa tttataccct
     3421 tgcaattcta tatcaaggaa atttttggct ggtcaactcg ataactgggg atgctcagca
     3481 aatcacaggc gac
//
LOCUS       DBNGSX010000443         5073 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_942821, whole genome shotgun sequence.
ACCESSION   DBNGSX010000443 DBNGSX010000000
VERSION     DBNGSX010000443.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_942821"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /gene="nrfD"
                     /locus_tag="AC16CB_13150"
     CDS             complement(<1..494)
                     /gene="nrfD"
                     /locus_tag="AC16CB_13150"
                     /inference="COORDINATES: protein motif:HMM:NF015851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR3343460.1"
                     /translation="MIATIKSIRWTPYTYWVTALLVCMFFGAIAGLIVFVRGLSVTNL
                     TDLVPWGLWITIDLSAIAMSAGAFLLCAAVYILDLKQYQPVARTATFIGLIGYSMAVL
                     TLLLDIGRPDRFWHAVVFWNFHSVLWEVTMCVTLYLCVLVLESAPIIGHTRWMQDRWP
                     KVAQ"
     gene            complement(491..1261)
                     /gene="dsrO"
                     /locus_tag="AC16CB_13155"
     CDS             complement(491..1261)
                     /gene="dsrO"
                     /locus_tag="AC16CB_13155"
                     /inference="COORDINATES: protein motif:HMM:NF045797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate reduction electron transfer complex
                     DsrMKJOP subunit DsrO"
                     /protein_id="HIR3343461.1"
                     /translation="MADQNIRRRDFIKLSAFGAAATAILRLARRVSASDPSGTSPHKW
                     AMVIDQAKCTGCGYCTLACRAHNDVPPDISWNRVIEVGNFGDRKSYLSRPCMHCEHAP
                     CVEVCPVKASYYRPDGIVMMNYDRCIGCRYCEVACPYGGRAFNWEKFTGPNPAVPKWG
                     QPEVERRPRGVVEKCSFCYQRIDRGLAMGLVPGVDDEATPACVVACPVRARIFGDLND
                     EESEIYKILSETPSYRLREDLGTMPRVYYLSARKEEAA"
     gene            complement(1264..2175)
                     /locus_tag="AC16CB_13160"
     CDS             complement(1264..2175)
                     /locus_tag="AC16CB_13160"
                     /inference="COORDINATES: protein motif:HMM:NF024827.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HIR3343462.1"
                     /translation="MLRFISRLWYGLLFAIPIMLITYALAEAANRPQETPTGELNCRE
                     CHESFVQAWETGSHGQATSDPAFREAWMAAGQSPECLSCHVTGYDEETNTWESDGITC
                     KACHSPITLNHPLAPMSSDRSANMCGDCHTETFFEWQISGHRNAGVSCSACHDPHATD
                     LKTTDKTLVCADCHRNRQSNFAHSQHSQAGLTCGDCHLQKISETGNEGKAKQSHSFIV
                     GLTACNSCHSYQMHDPVEVHPDASTPEPADAMSAVESLSVTPEPIPVSPMGYTTLSGL
                     IGVAFGVILAPYIERISRRNRDDKEEE"
     gene            complement(2965..3615)
                     /locus_tag="AC16CB_13165"
     CDS             complement(2965..3615)
                     /locus_tag="AC16CB_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3343463.1"
                     /translation="MKTRLLLVDDHVVVRQGLRILLENEPDFDIVGEADSASQAIIEL
                     TRLKPDVIIMDIGLPDISGIDGTREIKRISPGTAIVALTIHEDEEYFFKMLEAGASGY
                     VPKRAAPEELITAIRTAAQGEVYLYPSLAKLLVKDFLGQYQREKSEKMVNDLTPREME
                     VLAYLADGATNVDIAESLGISPKTVARHRENIMGKLNLHSRTELVKYAIRKGIIEP"
     gene            complement(3590..>5073)
                     /locus_tag="AC16CB_13170"
     CDS             complement(3590..>5073)
                     /locus_tag="AC16CB_13170"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3343464.1"
                     /translation="YIAFIFFYYGLAFFSMGIAILLELGHGTDERLRHSLRPLAAFGI
                     LHGANEWMEMFDKLGLLFGFPETKIAWEALRLIILAFSFLSLGAFGSSLLAENERSRR
                     LSLLLPLSLAAIWGLGLFSMRGIFSLVDIVDVTDVWTRYVLAVPSALFACAGLIVQQR
                     RFRQAGMAQFGRDSLWAAIAFFWYGIIGQTFTRASPLPPSTIINQGLFLEVFGFPVQL
                     LRAGAAVFAAVFVMRFLRSFEVEMQNQLAALHEARLQEAERREALRGDLLRRVVEAQE
                     AERQRIARELHDETGQALTALGLGLRGASATLKQNSRKAAEQLGQLETLVAQSLDELQ
                     RLIANLRPSHLDDLGLAATLRWYASEIQNRTPLEVNVEVRGQAHRLPTEVKTALFRIA
                     QEALTNTVRHAKAKSARVSLIFGNGDVTLQVSDDGQGFDVKNPSSSGRVSWGLEGMRE
                     RASLLGGDFQLFSEPRQGTLIEVTIPYNSAEMKELDEDSLASS"
BASE COUNT         1395 a         1335 c         1100 g         1243 t
ORIGIN      
        1 cattgcgcta cttttggcca gcgatcttgc atccaccgtg tatgccctat gatcggagcg
       61 gactccaata caagcacaca taagtacaga gtcacgcaca ttgtcacttc ccagagaacc
      121 gagtggaaat tccagaatac tacggcgtgc caaaatcgat cgggacgacc gatatccagc
      181 aacaaggtca aaacagccat gctataacca attagcccaa taaaagttgc cgtgcgggcg
      241 actggttgat attgttttaa atccaatata tatacagccg cgcataacag gaaagcccca
      301 gcgctcatgg caatagcaga gaggtcgatc gtgatccaca agccccatgg taccaaatct
      361 gttagattcg tcaccgaaag cccacgcaca aaaacgatca acccggcaat agcaccaaaa
      421 aacatgcaaa ccaacagcgc tgtcacccag tacgtgtaag gagtccagcg aatactctta
      481 atagtcgcaa tcatgctgct tcctcctttc tcgccgatag ataataaaca cgcggcatag
      541 tacctagatc ttctcgaagc ctgtaggagg gtgtctcaga taagatcttg tagatctcag
      601 attcttcatc attcaaatca ccgaatatcc gcgctcgcac cggacaagca accacacagg
      661 caggtgtagc ctcatcatca acaccaggaa ctaaccccat cgctaaccca cgatcaattc
      721 gctggtaaca aaaagagcac ttctcaacga caccacgcgg tcggcgctca acctccggtt
      781 gtccccattt tggcactgca ggattaggac cggtaaattt ttcccagttg aaagccctgc
      841 cgccataggg gcaagccact tcacagtacc ggcacccgat acaccgatca taattcatca
      901 tcacgatgcc atcaggtcga taatagctag cctttaccgg gcaaacctcc acacacggtg
      961 catgctcaca gtgcatacag ggtctagata agtaagactt tctatcacca aaattaccta
     1021 cctcgataac tcgattccag gagatatctg gaggaacgtc attgtgagcc cggcatgcca
     1081 acgtgcaata accgcatccg gtacacttgg cctgatcgat caccatagcc cacttatgag
     1141 ggcttgttcc actcggatca gaggccgaaa cccttcgtgc taaccgaagg atagccgtcg
     1201 cggcagctcc aaatgccgaa agcttgataa aatcgcgccg cctgatattc tggtcagcca
     1261 tgtttactcc tcctccttat catccctgtt gcgacggctt atacgttcga tataaggcgc
     1321 caggatcacc ccaaatgcca caccgatcaa tcctgataat gtcgtgtacc ccattggact
     1381 cactggaatc ggttcagggg ttacggataa ggactcaact gcagacatcg catcagctgg
     1441 ttcaggcgtt gatgcatcag ggtgaacctc taccgggtca tgcatttggt atgagtgaca
     1501 actgttgcat gcagtcaatc ccacaatgaa gctgtgcgat tgcttggcct tgccctcatt
     1561 tcccgtctca gatattttct gtaggtggca atcaccgcag gttaacccag cctggctgtg
     1621 ctgagaatgc gcaaaattcg attggcgatt acggtgacaa tcagcgcata ccagtgtctt
     1681 gtctgttgtt ttcaaatcag tagcgtgagg gtcgtgacat gcagagcagc tcacccccgc
     1741 attacgatgt ccactgattt gccattcaaa aaatgtctcg gtgtgacaat cgccgcacat
     1801 attggcggaa cgatccgaag acataggcgc aagaggatga ttcaacgtaa tcgggctatg
     1861 gcaggctttg caggtgatcc catccgattc ccaggtattg gtttcttcat cgtaacctgt
     1921 aacgtgacaa cttaagcact caggagattg gcctgccgcc atccatgctt ctcggaaggc
     1981 tgggtcagat gttgcttgtc catgactacc agtctcccat gcctgcacga atgattcgtg
     2041 acattcccta caattcaact cgccagttgg tgtctcctgg ggtcggttgg ctgcctcggc
     2101 caatgcataa gtaatgagca tgatcggaat ggcgaaaaga agtccatacc ataagcgcga
     2161 aataaatcgt aacatttttg cacctcttat ctatagatga ttatggtgaa gctggtattg
     2221 gtgctcttcg gaagacctta cagagaaggc aaccctgcat cagttgacca tgattgtttt
     2281 gccggtagta ttgccagcaa ggcacttcag gatgtttata tgcatcacag gttgcgcgac
     2341 gttcttggga gcacttatta atatcccaac atccaatatt tcgagctctc acttcaatcg
     2401 ggaccttctc agcttcagtt tgccagtgtt catccaatct tttgagcagg atgatcatca
     2461 acgcggtcaa gccgaatgga ataattattc gaaccacaag ccctatgatc atagctatta
     2521 gcgtggagag ccactccata aagcacctca atttttagaa taaataaaat tgtctgcgtc
     2581 catacctcga accgatgaaa agcaacaatc ctaagggaat aagtattctg acgaaaaaca
     2641 aaatggtgaa tgcaaggatt gtctccataa ctatctccct atccaaactt aaaatctatt
     2701 aatgttttct ctcagtatag gaatagagag ctgagatgta aatccagcag actaccaatc
     2761 tttttatgtg aatttatggt gataattgcc catgatcccc cgttaatagc ccccagattt
     2821 ccaatgggga catttatcca aatttagccc atgcatgtat cacgcacccc ataataaaaa
     2881 tggggcatat gtcacaaata aatgataggg agcttaccct ataaggcaag ctccctattg
     2941 tttttcatca ttgctagtta tcgattaagg ctcaataatc ccttttcgga tggcgtattt
     3001 aactaattcg gtacgcgagt gtaaattcaa tttacccata atgttttcac gatggcgagc
     3061 aacggtcttt gggcttattc ctagtgattc tgcgatatcc acgttggtcg ctccatcagc
     3121 taagtatgcc aatacctcca tttcacgggg ggtcagatca ttaaccattt tctcgctttt
     3181 ctctcgttga tattgcccta aaaaatcctt gaccagcaat tttgccaggg agggatagag
     3241 ataaacttcc ccttgggctg cagtccgaat ggcggtaatt aattcctcag gagctgcgcg
     3301 cttgggtaca tagccgcttg cgcctgcttc caacatctta aaaaaatact cttcatcttc
     3361 atggatggtt aaggctacga ttgctgtgcc aggtgaaatc cttttaatct ctcgcgtgcc
     3421 atcaatgcca gagatatccg gtaagccgat atccataata atgacatctg gttttagccg
     3481 ggtaagctcg atgattgcct ggctggcaga gtctgcctcg ccgacaatat caaaatcagg
     3541 ttcgttctcc agcaaaatgc gtaaaccctg tcggacaact acatggtcat caactagaag
     3601 caagcgagtc ttcatctaat tccttcattt ctgcactatt atagggtata gtcacttcga
     3661 tcagtgttcc ctgtctaggc tcagagaaaa gctgaaaatc gcctcctagc aacgaggcgc
     3721 gttcacgcat gccttcaagc ccccaagaca cccgtcctga ggatgaaggg tttttcacat
     3781 caaacccctg accatcgtct gagacttgca aagtaacatc tccattgcca aagatcaagc
     3841 tcactcgggc gcttttcgct tttgcatgcc ttactgtatt cgtcaaggct tcttgggcta
     3901 tgcgaaaaag agctgttttc acctctgtag gcaggcgatg agcctgtcca cgaacctcga
     3961 cattcacctc tagaggcgta cgattttgta tctctgaagc ataccagcga agagtggctg
     4021 ccaggcctaa atcgtccagg tgcgaagggc gaaggttagc tatcaatcgc tgcaattcat
     4081 ccagagattg cgccaccaaa gtttcaagct ggcctaattg ctcagctgcc ttgcgggaat
     4141 tttgcttcaa cgtagcgcta gcaccgcgta gcccaagacc taaagccgtt agagcctggc
     4201 ccgtctcatc gtgtagctcg cgagctatac gctggcgttc ggcttcctgg gcctctacaa
     4261 cccggcggag caagtcccct cgcaatgcct cacgccgctc agcttcttgc agccgcgctt
     4321 catgcagtgc ggcgagctga ttctgcatct cgacctcaaa tgagcgtaaa aaccgcatca
     4381 caaataccgc tgcgaatacc gctgctccag cgcgcagtaa ttggactgga aagccaaaca
     4441 cttctaggaa taatccttga tttattattg ttgagggagg caaagggcta gctcgagtga
     4501 atgtttgtcc gatgatccca taccagaaaa aagcaatggc tgcccatagg ctatcccggc
     4561 caaattgagc catacccgcc tgccgaaacc ggcgttgttg cacgatcaaa ccagcacagg
     4621 cgaataaagc agatggcaca gctaaaacat agcgcgtcca tacgtcggtg acatctacaa
     4681 tatctacaag agagaatatc ccacgcatac tgaaaagacc aagcccccaa atcgcagcca
     4741 acgataacgg caaaagcaaa ctcaggcgac ggcttcgttc attctcagct aagagcgaag
     4801 aaccgaatgc ccccagggaa agaaatgaaa aagctaaaat tatgaggcgt agagcttccc
     4861 aagctatctt tgtttccgga aagccaaaca gcaggccaag tttatcgaac atctccatcc
     4921 attcattggc accatgaaga attccaaagg ctgccagcgg tctaagagag tgtctcagtc
     4981 tctcatcagt gccatgccct aactctaaca atatggctat acccatactg aagaatgcta
     5041 gcccgtaata aaagaaaatg aatgcaatat aag
//
LOCUS       DBNGSX010000444        14452 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_42177, whole genome shotgun sequence.
ACCESSION   DBNGSX010000444 DBNGSX010000000
VERSION     DBNGSX010000444.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14452)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14452)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14452
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_42177"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="AC16CB_13175"
     CDS             complement(<1..563)
                     /locus_tag="AC16CB_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343465.1"
                     /translation="MKTLTTKEDVNSLLHASTAKAALGAAIKTGLLWMLAERPMTAEQ
                     VAQALNIPGKRGYYWLQYLQSFGVLESVPQGYVPSSVVRTAILETYSRESWQHLVIDE
                     QEKDACVHGLPQYISEPGSLWTVQGLEEPKNYVDRMRASLERAREFTIMLFEVHQNLA
                     NLVAELLDLTGVERMMDLGGNSGVVSMA"
     gene            complement(568..1563)
                     /locus_tag="AC16CB_13180"
     CDS             complement(568..1563)
                     /locus_tag="AC16CB_13180"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3343466.1"
                     /translation="MSQKVYVDYQKSTSPFLAGGDLINIEANKLQGVSTVDFHLTSRC
                     SQACPYCWGPRRFRNSVAKENAQSIIVRIKHLGVRRIVFTGGDPLQHPDVLDLIRYAK
                     DMGLETALSTTGDLVTPEVLQGLAPNLDLISLPLDGSTEEINAKTKHRGHYSAVMRSL
                     EWLRSYPGIDVKVCTPVTRHNLSDIPAIAQLVEGYSLTTQARVFYNVFQAFPRAMFSV
                     NWERLVVTDEEFAALAHQIGKGKNIRINFLDHDILDKLYVMVFPDGSMVIPKGADYLN
                     FGPFLEVKDFNSALKASQFDSAKHVRHSLGWGKAATPIRQALNVSLVDSFNILKV"
     gene            complement(1733..2257)
                     /locus_tag="AC16CB_13185"
     CDS             complement(1733..2257)
                     /locus_tag="AC16CB_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343467.1"
                     /translation="MLVPYLIVLRFFHVVASVCWAGGGFIFFLFVEPTAKALAPTGMQ
                     FVQYMVTKRRFSIFMVVSSTLTVLSGALLIWQLTGGQLIGYMKTGPGLGFSLGSIVGI
                     VVYFVGMFGINPRAIRLSKIGDEIQAAGGPPTPEQAAELHKLDKEMSALSLADFVLVA
                     LSLGLMATARYWVF"
     gene            2386..5043
                     /locus_tag="AC16CB_13190"
     CDS             2386..5043
                     /locus_tag="AC16CB_13190"
                     /inference="COORDINATES: protein motif:HMM:NF017539.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3343468.1"
                     /translation="MEDQNSPVIFSEWLKRRRKALDMTQDELAKRAGCSVGALRKIES
                     GDRRPSKQLADLLAKALEISEEDLQTFIRVARGELNLERLRPPALESLAPLPDISSLR
                     QTQPSASLDASTAPPSHRIPLQATPLIGRDNELAALERLFNDPQCRLLTLTGIGGIGK
                     TRLAIEFATQKLSVFPGGVFYIPLTPVNSPEKIVPAIADVLNIEFFGPSDLIEQFFYY
                     ISSHINQAVLFVLDNLEHLLVHEPTQDDKPGVVELVSEILQRLPNVKILGTSRERLNI
                     LGEWVYELHGLSVPQTNFTGNLEAYDSVALFVKSAQRIRTEFQVSAEDQLSLVRICQL
                     VEGAPLAIELAAAWVGILSCQEIAQEIKSNMDFLTTSMRDIPERHRSIRATFDHSWKL
                     LSDEERWALCQLSVFHGGFDRYAANQIAGASLPLLASLSDKSLVRRTASGRYDLHEVI
                     RQYSLSHLNEHPSKLETYQRHCEYYLKLLQEHETSLKDASQQEVIRQLTDEIDNIRAA
                     WAWAIQYKKYAELDQAGRGFGWYYEITGLYREGIEQLGSLVQALEAEPQGDKWHRVLG
                     LMLVHQALLHFRVGEFSRARNLYAQSIRILRPIGDQILLADGLIYLGILLHLMGEYEQ
                     ARSSVEEGLFFAKESNARWYEAYAIYNLGYIDSLMGRYEEGYEQMLVGLGIWRSLGDP
                     HYIALGLNFLVPTLNKLGRCEEAKTFMRESIALCEQSKNLWGLGTAYRYFGLACLAGG
                     QFTEAQTHIRKSLEIYGEFVVGYDIARSLAYLGDVAVMLGDLTTAKKDYLEALRISIE
                     EKVIPIALDALLGLGDLQAQLGKTEDALVLCYHILNHPASEDDTKSRVEKLRAALEPK
                     LNSKQVMAARTLATEKTFDVIVKEALETA"
     gene            complement(5146..5826)
                     /locus_tag="AC16CB_13195"
     CDS             complement(5146..5826)
                     /locus_tag="AC16CB_13195"
                     /inference="COORDINATES: protein motif:HMM:NF022441.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HIR3343469.1"
                     /translation="MNKTYTETETRQISDFDQVLIQGLAYSAQLIMSQGEQEELTIEA
                     PPDYLDRLRSEVKDRKLTVRLRGGWLQELEDALATCLNRPHIVYRLKVRELSHLEVQC
                     AYSIHASRIETPHLHIKLSGTGDFKLDWLVAETLEVHHSGCGIMRISGKVEEQTVVLN
                     GVGSYIAPSLDSQRARVRMTGTGLARIQVSQALDATLRGVGSLEYSGHPGVSKRISGP
                     GQILHITN"
     gene            complement(5895..6641)
                     /locus_tag="AC16CB_13200"
     CDS             complement(5895..6641)
                     /locus_tag="AC16CB_13200"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3343470.1"
                     /translation="MNTVNWIQSYTLLLRWQGLKFKTVLPFVIVAQFFTGIGTVIGLG
                     YLMPDIDSQTAMFLVTGGPTLSLVTLGLVMVPQMVAQAKTGGMLDYMRSFPVPRMAYL
                     AADLTIWLLSTLPGVLLALVVGSLRYNFELQFSLLIVPAFMLVVLMATSVGYAIALLS
                     PKAELVGVLTNLIIFSLFLFSPVNFPVERLPHWLETVHRFLPVKYAADAVRGALVPGY
                     SDGLGLALAVLAGWCLVGLVINYMVATRRG"
     gene            complement(6656..7591)
                     /locus_tag="AC16CB_13205"
     CDS             complement(6656..7591)
                     /locus_tag="AC16CB_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015786143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3343471.1"
                     /translation="MALLEIEHVSKAYRGGVLANDDISLSVGAGEVFGLLGPNGAGKT
                     TLVHQIIGLLAPTSGTIRVAGINTVESPGFARQACSFQPQTQVPIDGMTPYQAIELAG
                     RLRGGRTDEVRARTRALIDSFELGAWSNKSGEALSGGVRRLVAFCMAAVVPGQITILD
                     EPTNDVDPLRRRLLWQQVRQLADQGSAVLLVTHNVLEAERSVDRLAIIDRGKVIGSGT
                     PGSMKNGDGDYLRLELLLEPGTSLPLLPNFLSHTVNGGRRLIGRVSAEDVTKAAMWAN
                     RMKADKLVEEYSLGPATLEDAYIRKIGRADALETI"
     gene            complement(7717..10371)
                     /locus_tag="AC16CB_13210"
     CDS             complement(7717..10371)
                     /locus_tag="AC16CB_13210"
                     /inference="COORDINATES: protein motif:HMM:NF037660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3343472.1"
                     /translation="MSRSRLLQVLDAGLHRKLTLISAPAGYGKSTLLSEWASGCEWPL
                     GWVTLEAGDNDIERFLTYLMSAVQTAEESVPKLEDILGARFSLQPMSRDAMLAILVNQ
                     LSSTSERLVIVLDDYHLIENQDIHGFLNALLDNLPVNIHIVISTRTDPPLRLARLRAK
                     DQLNEITEKGLRFTPQEVETFFDVVMGLRLTDDEVAELEARTEGWVTGLQLVGLSLKD
                     REHPSELIHTLAGTHRYILDYLVEEVFSDLPPLLQTFLLRVSVLEHLSPELCDTVVGE
                     PDVPQQSKKILEFMDASNLFIVPLDSQRQWYRFHPLFADFLRDRLVAQHAEELPDLHR
                     RAAAWYASRGLLSEAIRHCFAGGDVDRAADLIQAEARDLLTRGEITTLLRWIDALPEE
                     VIAARPHLGLSRAWAMLMREPINFLDTIDQQIDQIAEGFGILSADLLSALAESEPESL
                     RRAGLSEFAMLLAFARRDNHDVDDTIKLFKAALEYMPESEHLLRGFTLAGLASTYARK
                     GAIKLAEDAFAQAAQHSLAANSIYGYVACTDWLATMQAEQGQLTRAAATYRQAIKLLT
                     SQAQYPLPLSGHVYVGLADVLLEQNDLQGAFENLQVGLQVGVQVRDVDALLNGYVIQA
                     RVLQMLNKGEEAQEAMQNAERQALETKSMSCVHDAQAHKARLALALGDVQVAQRWAAG
                     RGLGSVGDVHMEGPPNEIEQLTYARLLMAVGKASEALPILEALSGLQEQIGRTRALIE
                     SLTLQALCLRSLGRMDEAVRTLARALMLAAPEGFARVFIQEGPPLAALLRTVGSLGHS
                     PEYVKHLLEIFGEAPALQESVLDPLSERELEVLRLVADGLTNAEIAAQLVIAHSTAKT
                     HINRIYSKLGVSTRTQAVARARQLQILP"
     gene            11220..11702
                     /locus_tag="AC16CB_13215"
     CDS             11220..11702
                     /locus_tag="AC16CB_13215"
                     /inference="COORDINATES: protein motif:HMM:NF044704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HIR3343473.1"
                     /translation="MNFNEGDRVVHKKFGVGIVVSIEGMNLSGNGQSLYYRVDFSKTT
                     VWVPVSNQSEGLRLITPKSHLYRYRALLKSSPVSIDGDFHKRRSELEKRIDRGTFQGL
                     CEVIRDLNGRNAEKPLNYYEKTLLKRTREALISEWSITSGISRSETIDEINGCLEHRK
                     "
     gene            11712..12212
                     /locus_tag="AC16CB_13220"
     CDS             11712..12212
                     /locus_tag="AC16CB_13220"
                     /inference="COORDINATES: protein motif:HMM:NF044704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HIR3343474.1"
                     /translation="MSERGMSFQVGDRVIHWMYGLGEIIQLDEKVLSGHTGKYYVVQI
                     RDLTLWVPLSESGELCLRFPTPARDFKKLFHLLASPGESLSTERYLRKIQLTDLLKDG
                     TLESICRVVRDLVHFKRNNKLNENDKSTLERAKTFLLDEWSVVLSIPVQQAEIELTNL
                     LETSVV"
     gene            12308..12637
                     /locus_tag="AC16CB_13225"
     CDS             12308..12637
                     /locus_tag="AC16CB_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR3343475.1"
                     /translation="METKLYVGNLAFETTEEDLHTLFTQAGTVASVDLIKDRASGRSK
                     GFAFVVMGNQSELEKAIQMFNSYTLDQRQLTVNIARPREERSQGGNGGNWQGERKHTG
                     RGGSRRY"
     gene            12797..13909
                     /locus_tag="AC16CB_13230"
     CDS             12797..13909
                     /locus_tag="AC16CB_13230"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3343476.1"
                     /translation="MNFDSFAFHPRINSGISAAGYSIPTPIQMRAIPPILEGRDVLGL
                     AQTGTGKTAAFVLPILQRLLNGPRGRLRVLIISPTRELAEQTHKAIGTLGQQTRLRSM
                     TIYGGVSVQPQIQRLRTGVEIVVACPGRLLDLMGQRVINLNSVETLVLDEADQMFDMG
                     FLPDIRRILAAIPPSRQTLLFSATMPNEIRSLANEFLRQPVTVELGASRPVETVGHAV
                     YSVDQTRKFELLLAILRQSGNGQVLVFTRTKHRAKKLAGQLIHAGLPATSIQGNLSQN
                     RRQEAMDKFRSGRVKVLVATDIAARGIDISRISHVINYDMPDTAIAYTHRIGRTGRMA
                     KLGTALSLATQDDLPMIRTIERLLGRSLERRRLERG"
     gene            14206..>14452
                     /locus_tag="AC16CB_13235"
     CDS             14206..>14452
                     /locus_tag="AC16CB_13235"
                     /inference="COORDINATES: protein motif:HMM:NF046401.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343477.1"
                     /translation="MMQKHIEITRQRIQNFSGKIANLFYPQRAPVELRVYSAPDRISY
                     QDAMLGEYRKATVGEKFGPLWSTHWFHVKGQIPASWQD"
BASE COUNT         3527 a         3840 c         3698 g         3387 t
ORIGIN      
        1 gccatggaga ccacccctga attaccaccc aagtccatca tgcgttcgac acccgttaga
       61 tccagcagtt ctgcgaccag gtttgccagg ttttggtgga cttcgaagag cataatcgtg
      121 aattcacgcg ccctttcaag gctggctctc attctatcga catagttttt cggttcttcc
      181 aaaccctgaa cggtccacag agagccgggc tcgctgatat attggggcag gccatgcacg
      241 caggcatcct tttcctgttc gtcgatcacc agatgctgcc agctctcccg actataggtc
      301 tccagaattg cagtgcgcac cacagaggat ggcacatatc cctgcggaac ggattcaaga
      361 actccgaaag attgcaggta ctgcaaccag taatagccgc gtttgccggg tatattcaag
      421 gcttgtgcca cttgctcagc ggtcatgggt ctctctgcaa gcatccacag gagtcctgtt
      481 ttgatggctg ctcccagcgc cgcctttgca gtagaggcat ggagcaagct atttacatct
      541 tcttttgtag tcagtgtttt cattttttta caccttgagg atattgaagg agtcaacgag
      601 acttacattc agagcctgcc tgattggcgt cgccgcctta ccccacccaa gcgagtggcg
      661 cacatgtttg gcagagtcaa actggctggc tttcagagca ctgttaaagt cctttacctc
      721 aaggaaaggc ccaaaattta gataatctgc acccttgggg atgaccatgc tcccgtcagg
      781 gaaaaccatg acgtacagct tgtctagtat gtcatgatcc agaaagttga tgcggatgtt
      841 tttgcccttg ccaatttgat gcgccagcgc tgcaaactcc tcgtcggtca caaccagtct
      901 ttcccaattg accgaaaaca tcgcacgcgg gaatgcctga aagacgttat agaaaaccct
      961 cgcctgcgtg gtaagagaat acccttcgac cagctgggct atagctggaa tgtcgctcaa
     1021 attgtggcgt gtgactggtg tgcatacctt gacatcaatg ccaggatacg atcgaagcca
     1081 ctcgagtgat cgcatgacag ccgagtaatg cccgcgatgc ttggttttgg cgttaatttc
     1141 ttcggtggag ccatccaatg ggagagagat taaatcaagg tttggagcaa gaccttgcag
     1201 aacttcaggc gttacaaggt ctcctgtggt agagagcgcc gtctcaaggc ccatgtcttt
     1261 ggcatatctg atcaagtcca atacgtcagg atgttgaaga gggtcgccgc ctgtgaatac
     1321 aatccgccgc acgcctaaat gcttgatacg gacaatgatg ctttgggcat tttccttagc
     1381 cacagagttt ctgaagcgcc gcggtcccca gcagtagggg catgcctggc tgcatctact
     1441 ggtgagatga aaatcaacgg ttgaaacgcc ctgcagtttg ttggcctcaa tgtttataag
     1501 atcgccgcct gcgagaaatg gacttgtgga tttctgataa tcgacataaa ctttttgaga
     1561 catgggaaac ctcttgtgtg gcttttccgt ttgataatat ctcggattga ccgtgaagaa
     1621 acgtggttac tattccagac gagggagtta tttatgtcaa ggcttttctc agatcggcca
     1681 ggcatccaca tttgcctgac cgatctagta ggctgtggga tgcgtgcgct tgctaaaaca
     1741 cccagtaacg agctgtagcc atgagcccca gggacaaagc aaccagcaca aaatccgcca
     1801 ggcttagggc tgacatttct ttgtcaagct tgtgaagttc ggcagcttgt tcgggggtgg
     1861 gcgggcctcc cgcggcctgg atctcgtcac cgattttaga cagtctgatc gcgcgggggt
     1921 ttattccaaa catgccgaca aaatagacca caatgccaac tatcgagcca agggagaacc
     1981 caagaccagg acctgttttc atatagccaa tcaattgacc acctgttaac tgccaaatta
     2041 gcaacgcacc agacaacacg gtcaacgtgc tggagacaac catgaaaatg ctgaagcgac
     2101 gcttggtgac catgtactgg acaaactgca tccctgttgg cgctagcgct ttagccgtgg
     2161 gctccacgaa taagaagaat atgaacccgc cgcctgccca acatacgctg gctacgacat
     2221 gaaaaaaacg aagaacgatc agatacggga ctaacatgtt gacctccaat ttgatttgct
     2281 agtggattaa gtatgttgac cttattaaac aaccagtttt gggttgattg gggatagcca
     2341 ataccccaag cactacccca aaatgtgcta caatttttat ctaaaatgga agatcaaaat
     2401 tcacccgtca ttttcagtga atggctcaag cgtcgtcgaa aagcactcga catgacccag
     2461 gacgagttag caaaacgtgc tggttgctca gttggggctc ttcgaaaaat tgaatcgggc
     2521 gaccgaagac cttccaaaca actcgcggat ttactggcca aagcgcttga aatctcagag
     2581 gaagacctgc agacattcat acgcgtggcg cgcggagagt taaacctcga gcggctgcgc
     2641 ccccctgcac tcgaatccct ggctcctttg cctgatattt ctagccttcg acaaactcaa
     2701 cccagcgcgt ctctcgatgc gagtaccgcc cccccctcac atcgcattcc attacaagcc
     2761 acgcctttaa ttggccgtga taacgaatta gccgcgctgg aaagactatt taatgaccca
     2821 caatgtcgtc tgctcacgct gacaggcatt ggcggcattg gtaaaactcg gcttgcgatt
     2881 gaatttgcga cgcaaaagct ttcagtattc cctgggggtg ttttctatat tccgcttacc
     2941 cctgtgaatt cccccgagaa aattgtcccc gccatagcag atgtgctgaa tatcgaattt
     3001 ttcggaccca gcgacctaat agaacaattt ttctattaca tatcaagcca catcaaccaa
     3061 gcagtgcttt ttgtcttgga taatttggaa catctactcg tccacgaacc tacacaagat
     3121 gacaaacctg gtgtggttga attggtttca gagatacttc agcgattgcc aaatgtcaaa
     3181 atcctgggaa cgtctcgcga gcgcttgaat atccttggag agtgggtata tgaactccat
     3241 ggactctcgg taccccaaac caattttacg ggaaacttgg aagcgtacga ctctgtcgcg
     3301 ctatttgtga aaagcgctca gcggataaga acagaatttc aagtatccgc agaagaccaa
     3361 ttatctctgg ttcgaatttg tcaattggtg gaaggagctc ccctagctat tgaactggcg
     3421 gcggcatggg tgggcatact ttcctgtcaa gagatcgcgc aagagattaa atccaatatg
     3481 gatttcctga ccacctccat gcgcgatatt cccgagcgcc accgctcaat cagagccacc
     3541 tttgaccatt catggaaatt attgtcagat gaagaacgtt gggctctttg ccagctatca
     3601 gtttttcatg gaggcttcga tcgttatgcg gcaaaccaga ttgcaggagc atcgctgccc
     3661 cttctggctt ccctgagcga caaatcgctc gtgcggcgca ccgcgagtgg acgttacgat
     3721 ctgcatgagg taatccgcca atattccttg tcccatctaa atgagcatcc gagcaagctt
     3781 gaaacttacc agcggcattg cgaatattac ctgaaattgt tacaggagca tgaaacctcg
     3841 ttaaaggacg cctctcagca agaagtcatt cgacaattga ccgatgagat agacaacatc
     3901 cgcgctgctt gggcatgggc aattcaatac aaaaaatacg ccgagctcga tcaggctggg
     3961 cggggcttcg gctggtacta cgaaatcaca ggcttatatc gtgaggggat cgaacagctt
     4021 ggttcgctcg tgcaggcatt ggaggctgag ccacaaggtg ataaatggca cagggttttg
     4081 gggctcatgc ttgtccacca ggcattgcta catttccgag tcggcgaatt cagtcgtgcc
     4141 cgaaatcttt atgcacaaag catcagaatc cttcgcccaa ttggcgacca gattttgttg
     4201 gccgacgggc tgatttattt aggtatcctc cttcatttaa tgggtgaata tgaacaggca
     4261 agatcatcgg tagaggaagg attatttttt gccaaggaga gcaatgcacg ctggtatgaa
     4321 gcatacgcta tctacaatct tggctacatc gatagcctga tgggacgtta tgaagagggg
     4381 tatgagcaaa tgttggttgg cctgggcata tggcggtcac tcggcgaccc gcattacatc
     4441 gccctggggc taaacttcct tgttccgacc ctaaataaat taggccgctg tgaagaagcc
     4501 aaaaccttca tgcgggaaag catcgcactt tgcgagcaat caaaaaatct ttgggggttg
     4561 ggcactgcat atcgctactt tggattggcg tgtctcgcag gaggtcaatt cacggaggcg
     4621 caaactcata ttcgcaaaag cctggagatt tacggtgagt ttgtcgtggg ctatgacatt
     4681 gcccgctctc tcgcctatct gggggatgtc gccgtgatgc ttggagattt aaccacggcc
     4741 aaaaaagatt atctggaagc cctacgcata tctatagaag aaaaagtgat tccaatcgca
     4801 ttggatgcat tattgggttt gggcgacctg caagctcaac ttggcaaaac cgaagatgcc
     4861 ctcgtacttt gctaccatat cctgaatcac cccgctagcg aagatgacac aaaaagccgc
     4921 gtggaaaaac tacgcgcagc cttggagccc aaattgaact ccaaacaagt catggcagcg
     4981 cgcactttgg caaccgagaa aacgtttgac gtcatcgtga aagaagcctt ggaaacggca
     5041 taaggatatt gcaggcataa agccttgcgt tgtgccaggt agtatgctca gctcaattcc
     5101 cttcgcttcg cactgccagc ccattggctg gggatttctt tctcactaat tcgtgatatg
     5161 cagtatctgt cctggaccag aaattcgctt gcttacgccg ggatgcccgc tatattccaa
     5221 gctacccacg ccgcgcagcg tagcgtccag cgcctggctg acctggattc tggctaaacc
     5281 tgtgcctgtc atgcgcacgc gggcgcgctg gctgtccaac gaaggggcaa tgtagctgcc
     5341 tacaccgttc agcacgacag tttgctcctc cacctttcct gagatgcgca tgatcccgca
     5401 gccggagtgg tgcacttcca aggtttcagc cacaagccag tcaagcttga aatctccagt
     5461 cccgctcaat tttatgtgaa ggtgcggagt ctctattctg gatgcatgta tgctgtaggc
     5521 acactgcact tccaggtggc tgagctcgcg caccttcaac cggtagacga tgtgcggcct
     5581 gttcaggcag gtcgcaagag catcttctaa ttcttgcaac cagccgcccc gcaagcgcac
     5641 ggtgagcttt ctatctttca cctcgctgcg caacctatcc aggtaatctg ggggtgcttc
     5701 gatggtcagc tcttcctgct caccctgaga catgatgagt tgcgcgctgt aagccaaacc
     5761 ttggatcaat acctggtcaa aatcgctgat ctgccgggtc tctgtttctg tgtacgtttt
     5821 gttcatgata gttatcgttg ttagaaacag caatttatag taattccaat tgtttgcacc
     5881 gggcgtactg gctatcaacc ccggcgggta gctaccatat aatttataac cagccctacc
     5941 agacaccaac cagccagcac agccagcgcc agacccaatc catccgaata acccggcacc
     6001 agcgcgccgc gcaccgcatc ggcagcatac ttcaccggca agaagcgatg gactgtctca
     6061 agccagtggg gcaggcgctc gacggggaag ttgaccggcg agaacaggaa caggctgaag
     6121 ataattaagt tggtcagcac gcccaccaat tccgctttcg gcgagagcaa ggcgatggcg
     6181 tagcccactg aagtcgccat cagcaccacc agcatgaaag ccggcacgat caacaggcta
     6241 aattgcagct cgaaattgta gcgcagcgag cctacaacaa gcgccaggag cacgccaggc
     6301 agggtggaca gcaaccatat ggtcaaatct gccgccaggt aagccatgcg tggcacagga
     6361 aaggagcgca tatagtccag catcccaccg gttttcgcct gtgctaccat ctgcggaacc
     6421 atcactaatc ccagtgtgac cagcgagagt gttggtccgc cagtcaccaa gaacatggct
     6481 gtttgggagt cgatgtctgg catcagatag cctaggccga tgaccgtacc gataccggtg
     6541 aagaattgag cgacaattac aaacggcagg acagtcttga acttcagccc ctgccatctg
     6601 agcaataggg tgtagctttg gatccaatta actgtgttca tttgatatat ctccttcaga
     6661 tagtctccaa ggcgtctgcg cgcccgattt tacgaatgta tgcgtcctcc agggttgccg
     6721 gcccaaggga gtattcctcc acgagcttgt cggctttcat gcggtttgcc cacatggctg
     6781 ccttagttac atcctcggcg ctaactcttc cgataagacg ccgcccaccg ttgaccgtgt
     6841 gcgataggaa gtttggcagc aggggcagcg aagtcccggg ctcaagcagc agctccaggc
     6901 gcaagtaatc cccgtcacca tttttcatgg aacccggcgt tccagaaccg atgactttgc
     6961 cacggtcgat gattgccaaa cgatcaaccg agcgctccgc ctccagcaca ttatgggtaa
     7021 ccagcagcac cgcgctaccc tgatctgcta gctggcgcac ctgctgccac agcaagcgcc
     7081 gtcgcagcgg atcaacatcg ttggtaggtt cgtccaagat ggtgatctga ccaggaacga
     7141 ccgctgccat acagaaagcc accagccgtc gcacgccccc tgagagagcc tcaccacttt
     7201 tattcgacca cgcgccgagc tcaaagctgt cgatcagcgc tcgggtcctg gcgcgcacct
     7261 cgtccgtacg accgccgcgc aggcgtccgg ctagctcgat agcctggtaa ggtgtcatac
     7321 catcgatggg aacctgtgtc tggggctgaa acgagcatgc ctggcgggca aaaccagggg
     7381 actcgaccgt gttgatgcca gccacccgga tcgtgcctga ggtgggtgcc aacaaaccga
     7441 tgatctggtg taccagggta gtcttgccgg cgccgttggg tcccagcagg ccgaacacct
     7501 cacccgctcc aaccgacagg ctgatgtcgt catttgccag caccccgccg cggtaggctt
     7561 tacttacgtg ttcaatctct agcaatgcca tcttctttcc tccaaaacaa ataaactcat
     7621 ggtgtggata atctttcgcc atgagtttac aaaaaaagtg gaggaaaagc gtccacccgg
     7681 gggtggattg gggggtggaa tgaaaaaggg ggaggattaa ggcaaaatct ggagttggcg
     7741 ggcgcgggcg accgcctggg tgcgggtgct gacgccgagt ttgctgtaaa tgcggttgat
     7801 gtgggttttt gccgtgctgt gggcgatgac cagttgagcc gcgatctcgg cgttggtcag
     7861 cccgtctgct accaagcgca gcacctccag ctcgcgctcg ctgagtggat caagcacgga
     7921 ttcctgcaat gcgggggctt cgccgaagat ctcaagaagg tgcttgacgt attccggcga
     7981 gtggcccagc gacccaaccg tgcgcagcaa cgccgccaac ggaggccctt cctggataaa
     8041 gacgcgagca aaaccttccg gcgcggcgag catcagggca cgcgccagcg tgcgcaccgc
     8101 ctcatccatg cgtcccaagg aacgcaggca tagcgcctgg agcgtcagac tctcgatcag
     8161 ggcgcgcgtg cgcccgattt gttcttgcag gccactcaaa gcctccaaga tgggaagcgc
     8221 ctcagatgcc ttccccactg ccatcagcaa gcgggcatag gtcagctgtt cgatctcatt
     8281 tggaggaccc tccatatgaa cgtctccaac gcttcccaag ccacgccccg cagcccagcg
     8341 ctgggctacc tggacatctc caagagccag ggcaaggcgc gccttatgtg cttgcgcatc
     8401 atgcacgcag ctcatgcttt tggtctctag ggcttgtcgc tcagcatttt gcatggcttc
     8461 ttgcgcttct tcccccttgt tgagcatttg taaaacgcgt gcctggatta catagccatt
     8521 caacagggca tccacatccc tgacctgaac cccaacctgc agccccactt gcagattttc
     8581 gaatgcccct tggagatcat tctgctccag cagcacatcg gccagtccga cgtagacatg
     8641 ccccgaaaga ggtagcgggt actgggcctg actggtgagc aacttaatcg cctggcgata
     8701 agtggcggcg gcgcgcgtga gctgaccctg ctcggcttgc atggttgcca gccagtcggt
     8761 gcaggccaca tagccataga tgctgttggc tgccaggcta tgctgtgctg cctgtgcaaa
     8821 cgcatcctca gccagtttga tcgcgccttt cctggcatag gtcgacgcca acccggctag
     8881 agtaaaaccg cggagcagat gttcgctttc tggcatgtat tcaagcgcgg ctttgaacag
     8941 cttgatcgta tcatcaacgt catggttgtc acgccgcgca aatgccagaa gcatagcaaa
     9001 ttcactcagc ccggctcgcc gaagcgattc gggctcgctc tcagccaggg cactgagcag
     9061 atctgcagac aggatgccaa aaccttcagc gatctggtcg atttgctggt cgatcgtatc
     9121 caggaagttg atcggctcac gcatgagcat cgcccaggcc cgcgacagcc ctaaatgagg
     9181 acgggcagca atgacttcct ccggcaacgc atcaatccag cgcagcaggg tcgtgatctc
     9241 cccgcgggta agcaggtctc tggcctctgc ctggatcaag tcggcagcgc gatcaacatc
     9301 cccacctgca aaacagtgtc ggatcgcttc tgagagcaag ccgcgactgg cataccaggc
     9361 agctgcccgg cggtgcagat cgggcagctc ttccgcatgc tgagccacca agcggtcgcg
     9421 caggaaatca gcaaacagcg gatgaaatcg gtaccactgg cgctggctgt caagcggcac
     9481 gatgaacagg ttggatgcgt ccataaattc caaaatcttc tttgactgct gcggcacgtc
     9541 cggttcccct accacggtgt cacacagctc aggcgacaga tgctcaagga ccgaaacacg
     9601 cagcaggaat gtctgcagca ggggaggaag gtctgagaac acctcctcca ccaggtaatc
     9661 caagatgtag cgatgagtgc ctgccagtgt atggatcagc tcgctgggat gctcgcgatc
     9721 cttcaaagaa agacccacga gctgcagccc agtaacccag ccttcggtgc gcgcttccag
     9781 ttcggcaacc tcatcatcag taagcctcag ccccatcacc acatcgaaaa aggtctccac
     9841 ctcctgaggc gtgaagcgca gccccttttc ggtaatctca ttcagttgat ccttggcgcg
     9901 taaacgagcc agccgcaaag gcgggtccgt tcgtgtggag atcacgatgt ggatattcac
     9961 gggcaggttg tcgagcaagg cgttaagaaa tccgtggatg tcttgatttt cgatgagatg
    10021 gtaatcatcc agcacgatca ccagccgttc tgaagtggag gataactggt ttaccagtat
    10081 cgccagcatc gcatcgcgcg acatcggttg gagtgagaag cgagctccca ggatgtcctc
    10141 cagtttaggc acgctctctt cggcggtctg tacagcagac atcaggtagg taagaaagcg
    10201 ctcgatgtcg ttatcgcctg cttcaagcgt gacccagccc agcggccatt cacagcccga
    10261 agcccactcg ctgagcagcg tcgacttgcc atacccagct ggtgccgaga tgagggttag
    10321 cttgcggtgc aaacctgcat cgagaacctg gagcaagcgg ctgcgcgaca cgagctgcag
    10381 cctagaaggc gggatctgca ccttcgggag caggatggga aggggtgtag gaatatccat
    10441 gagcaggtat agcggtggcc taatataatg ggtttgctgg ttgaacgatt atagcactac
    10501 ctgctcagct atactgcctc ttgctcattg atgaaattat tgccttcaaa tagcaaaatt
    10561 gcacctacct atttcattcc ctgacccagc ctcctcccaa cgtctttaca cccgaccttc
    10621 cgttgaggtt caccagatga tgttgctgtc tgcgggctac tttgtagttt agcagttcgc
    10681 tccaggcgtg cacaaggggt tggtcatctt actgctttgt tctctatcat cacggccttc
    10741 aattaatcca cgcaaatttg agcgaaaatt ctggaactta acaggtaatt tttacgattt
    10801 atcctacacc aactcacaac ctctagcacc ctgccgaggc taccccttga aagactcctg
    10861 aaccgtcgaa ttgtacaggt ctacagggca ggatgggcat accccccaaa aataccccaa
    10921 acactctttt tacgggattg cgtatgcccc tcagggcata tgcaattgcc ctggagtcac
    10981 aatttgccca ggtcagtaat atgatataat ttatataatc ttacttggga tatgtggaat
    11041 cgccgctagt cctcgtgcag gataggcggc tgtttttttc gctacttctg ttgactagcc
    11101 gattgaatat ttaatcccga gatagcaatt tagcagattg cttgggggcg agatgatcca
    11161 ttggaggcct attattttta cgcatctctg tttcggttta tcagcctgtg gatacaaaaa
    11221 tgaacttcaa tgaaggcgat cgtgttgttc acaagaaatt tggagtagga attgttgtat
    11281 cgatcgaagg catgaattta tccggcaacg gacaaagcct ctattatcgt gtagattttt
    11341 ccaaaaccac cgtttgggtg ccagtaagta atcagtcaga agggttgcgg cttatcacac
    11401 caaagagtca tctctatcgg taccgcgcct tactaaaaag ctcaccagtc tcaatagacg
    11461 gcgatttcca taaacggcgc agcgaattgg aaaaacggat tgatagaggt accttccagg
    11521 gtttatgtga ggttattcga gacctcaatg gacgcaatgc tgagaaacca ctgaactact
    11581 atgagaaaac acttcttaag cgaaccaggg aagctctgat ttcagaatgg tctattacga
    11641 gtgggatctc tcgctccgag acaatcgatg aaattaatgg gtgcttggag catagaaagt
    11701 gagttcagtc catgagtgaa cggggtatgt ctttccaggt tggagaccgg gtgatccatt
    11761 ggatgtacgg tctcggagaa attatccagc tggatgaaaa agtgttgtct ggacacactg
    11821 gcaaatatta tgttgtgcag attcgcgatc taacactttg ggttccgcta agcgaaagtg
    11881 gtgagctctg cctgcgtttc ccaaccccag caagagattt taaaaaactc tttcatcttc
    11941 tagccagccc cggagaatcg ctatcgactg aacgttatct acggaaaata cagttgacgg
    12001 atcttttgaa ggacggaacg ctcgaatcga tttgccgggt ggttcgcgat ctggtgcatt
    12061 ttaaacggaa caataaatta aatgaaaatg acaaatctac cctggaacgc gccaagactt
    12121 tcctgttgga tgaatggagc gtggttttat cgattccggt ccaacaagct gagatcgagt
    12181 tgacgaactt attggagaca agtgtggtat aatttattat cttactactg ggtagagtta
    12241 ccgtcggccc cttctgtgag gctggcggtt ctttttttta atacatctca aaggagatac
    12301 acggattatg gaaaccaagc tttatgtcgg taatcttgct tttgaaacta ccgaagaaga
    12361 tttgcacacg ttgtttactc aggctggtac agttgcctct gtagacctga taaaggatcg
    12421 tgcaagcggc aggtcgaagg gttttgcctt tgtagtgatg ggcaatcaaa gtgaattgga
    12481 aaaagccatt caaatgttca acagttacac cctcgaccaa cgccaattaa ctgtcaatat
    12541 cgctcgtccg cgcgaggagc gcagccaggg cggaaatggc ggcaactggc aaggagagcg
    12601 taagcacacc ggtcgcggcg gttcccgtcg atattaagcc ttagcatgta atgttgatag
    12661 aagggctcca agccggtttg cgcgcctgga gcccaaaccc actatataaa cgactttcag
    12721 gacactttgc agttcgagca ggacacctgg acgtatggtc tcgaacgagt tcctatttgt
    12781 cgttaaagga tctgctgtga attttgactc atttgctttt catccccgga ttaattccgg
    12841 aatctcagcc gctggctact ccatccctac cccgattcag atgcgtgcca ttccacccat
    12901 cttggaaggg cgtgatgtgc tgggcttggc ccaaaccggc accgggaaaa cggctgcttt
    12961 tgtattaccc atcctccaac gcctcttaaa cgggccacgt ggcaggttgc gtgttctcat
    13021 catatctccc acgcgcgagc tggccgaaca gacccataaa gccattggaa cgctcggtca
    13081 gcagactcgt ctccgaagta tgaccatata tggaggcgtc agcgtccaac cgcaaatcca
    13141 gaggttgcgt actggagttg aaatcgttgt ggcctgccca ggccgcttgc tggatctgat
    13201 ggggcagcga gtgatcaacc tgaattcggt tgaaaccctg gtgttggatg aagctgatca
    13261 gatgttcgat atgggttttt tgcccgacat ccggcgcatt ctggctgcta ttccaccatc
    13321 acggcaaaca ctgctcttct cagccaccat gccgaatgag atacgttcgc tagccaatga
    13381 attcttgcgt caacctgtga cagtcgaact gggcgcatct cgtccagtcg aaacggttgg
    13441 tcatgcggtg tattcggtgg atcaaaccag gaaatttgag ctgcttctgg caatcttacg
    13501 ccaatcaggc aacggccaag ttctggtatt cacccgtaca aaacaccgcg ccaaaaaact
    13561 cgccgggcaa ttgatccatg ctggactgcc cgctacatct atacaaggca atctatctca
    13621 gaaccggcgc caggaagcga tggacaaatt tcgcagcggc cgggtcaagg tattagttgc
    13681 tacagatatt gccgcaaggg ggattgatat ttctcgtatc tctcacgtga tcaactacga
    13741 tatgccggac acagcgatcg cctataccca ccggattggt cgaacaggtc gcatggcgaa
    13801 gctgggcacg gcgctatctc tcgccaccca ggatgacttg cccatgattc gtacgattga
    13861 gcggcttctg gggcgttcgc tggagcggcg gaggcttgaa agaggataga ttacgggata
    13921 ttatcctcga ctggcaatct aaaaacgcct gctcgaataa ttacggcggg gagataggat
    13981 attctgcgca gagagcaacc atcgtctcca cagataaaat ccgccaatct acgcgaattt
    14041 ttacaaatat tcgtggagat tggcggataa tttattccaa ggagcctacc ccagcacaag
    14101 ccctttcgaa aaatctctcc aatacgcgga cctgcattta taatcagctt gacgacaaag
    14161 ccaaaaagcc atgattgttc aggatagata ctcttcgcca aggacatgat gcaaaaacat
    14221 atcgagataa cccgtcaacg tatacagaat ttttccggca aaatcgccaa ccttttctat
    14281 ccgcagcgcg ctccggtaga gctaagggtc tactctgccc ccgaccggat ttcctatcag
    14341 gacgccatgc ttggcgagta ccgtaaagca actgtcggtg aaaagttcgg gccgctatgg
    14401 tcaactcact ggttccacgt caaagggcaa ataccggcgt cttggcagga tc
//
LOCUS       DBNGSX010000445         6214 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1912444, whole genome shotgun sequence.
ACCESSION   DBNGSX010000445 DBNGSX010000000
VERSION     DBNGSX010000445.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6214)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6214)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6214
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1912444"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="AC16CB_13240"
     CDS             complement(<1..797)
                     /locus_tag="AC16CB_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3343478.1"
                     /translation="MSDIAIRVENLGKQYVIGAQIDRYRTLRDTLVEVIKRPVRMLRG
                     GSGNTNDTIWALSGVSFEVRKGEVLGVIGRNGAGKSTLLKILSRVTEPSEGFAEIHGR
                     VGSLLEVGTGFHPELTGRENIALNGAILGMKRQEIERKFDEIVDFSGIEKFIDTPVKR
                     YSSGMYLRLAFAVAAHMEPEILVVDEVLAVGDAEFQRKCLGKMSDVASQGRTVLFVSH
                     NMSAILRLTQETILLEKGKLVLRAPSSQAVDAYLTSGFAQSGERYWQP"
     gene            complement(816..1664)
                     /locus_tag="AC16CB_13245"
     CDS             complement(816..1664)
                     /locus_tag="AC16CB_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3343479.1"
                     /translation="MAEISQTAITEEVIVLRPSRGWSALNLRDLWLYRELIYFLTWRD
                     IKVRYKQTVLGAGWAILQPLINMIVLGVIFGNFAKMPTEGIPRPIFTFAALLPWGLFS
                     KALNDAGRSMLSNRNMITKVYFPRLIIPLASVLGGVVDFLIQFAILILMMVYYGYAPT
                     ANIWTLPLFLVLALITALGFGLWLSALNVLYRDVNYILPFLTQLWVLVTPVAYSSSVV
                     PEQWRMIYALNPMVGVVEGFRWALLGTETAPGPQMILSVVISLVLLITGMYYFRRMER
                     TFADMV"
     gene            complement(1726..2703)
                     /locus_tag="AC16CB_13250"
     CDS             complement(1726..2703)
                     /locus_tag="AC16CB_13250"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase family protein"
                     /protein_id="HIR3343480.1"
                     /translation="MSDIQSSNYWLNKRVIVTGGAGFLGSFLVEKLKARGAAEVLIPR
                     VEQYNLVDIADIRRMYDDYLLPTASNLQTIVIHLAALAGGIGANRSRPADFFYINLMM
                     GVQLMHEAWIRHIDKFVAIGTICAYPKFTPMPFREENLWDGYPEETNAPYGLAKKMLL
                     VQAQAYREQYGFNAIYLLPVNLYGPRDNFDLQTSHVIPALIRKFIEAQERGDRQVVLW
                     GDGSPTREFLYVEDACEGILLAAERYDSAEPVNLGSGMEISIKDLAHLIARLTSYTGE
                     FVWDTSKPNGQPRRALDVTRAEKHFGFRAQMPFEEGLRRTIEWYRKNKG"
     gene            complement(2751..3800)
                     /locus_tag="AC16CB_13255"
     CDS             complement(2751..3800)
                     /locus_tag="AC16CB_13255"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3343481.1"
                     /translation="MKASSSRLDFGSEPAQRHILVTGGAGYIGSLLTGELLRVGYRVT
                     VLDDLLFGGESLLAYMSHPDFHFVKTNIWEPRAVRNAVRDDWSRPAAVVHLAAIAGFP
                     ACQAVGRQVAWRYNVEATQQVYEQAVELGVGRFIYASTYSNYGLSPDGNPVTEDTPLN
                     PQSLYAETKIAAERYLLGQASNPCAPLIFRLATLYGISPRTRFDMIVNQFILEAYTRR
                     ELLIYQRGYSRSFMHIRDAVRGILLGLKAPEADIRGQVYNLGTKGGNATKDQVVSLIL
                     KRLPETVVRYKDMTFGGDMRDISVSFEKVQSKLGFEAAYTLDDGVREVLHALRTGLIP
                     DPQNPRYRNAQFIVQ"
     gene            complement(3790..4287)
                     /locus_tag="AC16CB_13260"
     CDS             complement(3790..4287)
                     /locus_tag="AC16CB_13260"
                     /inference="COORDINATES: protein motif:HMM:NF024212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3343482.1"
                     /translation="METPPETIHELNLLEHIEDDPDITQASLASQLGVAVGTVNWYLK
                     RLVAKGYVKVKRAQRRKLRYIITPEGIAFRARLTLHYIDTSMKLYRRTRQHVRELLGD
                     VKKAGYNRVHIIGEGDIADVCRLTCLEQGISVAEMIDGKDADLPVIELHGMKVHLRLE
                     KVHES"
     gene            complement(4341..6068)
                     /locus_tag="AC16CB_13265"
     CDS             complement(4341..6068)
                     /locus_tag="AC16CB_13265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR3343483.1"
                     /translation="MIRLLRPFVNRRGWMGTAIFVGVLLMLWLTRGLALDRFVTVDEP
                     KWLLRSANFYNALAHSNFKDTFQREHPGVTITWAGSAGFLWRFPGYVKISPEGNMTPT
                     RFHTFLRSYGRSSLELLVASRTFVLLGIVLALGLSFLIAVRLLGTLPAFLAFLLIAFD
                     PFSIALSRMLHLDGLLSALMLLSLLSFLAYLFLNRRMGYLLLSAVAAGLAWLTKSPAF
                     FLAPFFGLLLLIEWLQSGRRTPIRDLFRKRHLWQAFSPFLLWFAVAWLVFILLWPAMW
                     VDPVGSLTSVFSQATAYASEGHEIPSYFYGETQLGGISSWYFYPLVYLWRITPFILVG
                     LVFVLLALFFPKVFPLENTRLILVLLLFTGLFTIFMSLGAKKFDRYLLPIFAPLDLIA
                     ALGWLALLKAANRYAKRLSTISRRLAGGLLLGIVLLGQMWSVIQSFPYYLNYFNPILG
                     GSKAATQVMMVGWGEGLDQAARYLNSLPQTGKERAISWYGDGCFSYFYDGVTVPIGLD
                     FTFHDLRKTDIVVLYLNQWQRGLPSPEFMAYFEQLTPDHVVRINNLEYVRVYNLRNAP
                     EMPILTPGE"
BASE COUNT         1513 a         1678 c         1731 g         1292 t
ORIGIN      
        1 ggttgccagt atcgctcgcc agattgggca aagccagatg tcagataggc atcaacagct
       61 tgagacgaag gggcgcgtag caccagcttg cctttttcca acaggattgt ctcctgggta
      121 aggcgcagga tggctgacat gttgtggctg acgaacagca ccgtgcgacc ttggctggcg
      181 acatcgctca tcttgcccaa gcatttgcgc tggaactcgg cgtcacccac cgccagcact
      241 tcatcgacca ctaaaatctc cggctccata tgagcagcga cggcgaaagc caggcgcaaa
      301 tacatgccgc tggaatagcg cttgacgggg gtatcaatga acttctcgat ccctgaaaag
      361 tcgacaattt cgtcaaactt gcgctctatc tcttgccgtt tcatgcccag gatagctcca
      421 tttagggcaa tattctcgcg tccggtcagc tctggatgga aacccgtgcc tacctctaga
      481 agggacccca cccgcccgtg aatctcggcg aagccctcgc tgggctccgt gacgcgcgaa
      541 aggatcttga gcagggtgct cttgccggcg ccgttgcgcc cgattacgcc cagcacctcg
      601 cctttgcgca cctcaaatga gacaccgctc agcgcccaga tggtatcgtt ggtgttgcct
      661 gacccgccgc gcagcatgcg cacgggacgc ttgatcacct ccactagggt gtcgcgcagc
      721 gtccggtagc ggtctatctg cgcaccaatc acgtattgct tgccaaggtt ttctacgcgg
      781 attgcaatgt cactcataca ggttctctgt tccctttaca ccatatcggc gaacgtccgc
      841 tccatgcggc gaaagtagta cattccggtg atcagcaaga ccaacgagat gaccaccgat
      901 aatatcatct gcggaccagg ggctgtctcc gtgcccagca aagcccagcg gaagccctca
      961 accacaccca ccatgggatt gagggcgtat atcatgcgcc actgctccgg gacgacgctg
     1021 gaggaatacg ccactggcgt gacaagcacc caaagttgcg tcaggaaggg caggatgtag
     1081 ttcacatcgc gatagagtac attcagggct gaaagccaca ggccaaaacc taacgccgtg
     1141 attagcgcca gcaccaggaa caaaggcagc gtccagatat ttgcggtggg agcgtagccg
     1201 taatagacca tcatgaggat caagatggcg aattggatca agaaatccac cacgccgcct
     1261 agcacagacg caagagggat gatcaacctg gggaaataga ccttggtgat catgttacgg
     1321 ttggagagca tcgatcgtcc cgcgtcatta agcgctttgg agaagagccc ccagggcagc
     1381 agggcagcga aggtgaagat tgggcggggt ataccttccg tgggcatctt ggcaaagttg
     1441 ccgaaaatca ctcctagaac gatcatgttg atcaacggtt gcaagatagc ccagcccgcc
     1501 cccagaacgg tctgtttgta gcgcaccttg atatctcgcc aagtgaggaa gtagatcagc
     1561 tcgcggtaga gccaaaggtc gcgcaggtta agcgccgacc agccccgcga cgggcgcagg
     1621 acaatgacct cttcggttat tgctgtttgt gatatctctg ccataacaca ttccgtgaag
     1681 aaggggataa gggatagggg ataggggaaa ctgatcccct atcccttatc ccttgttttt
     1741 tcggtaccat tcaattgtcc tgcgcaaccc ttcctcgaag ggcatttgcg cccggaagcc
     1801 gaagtgtttc tcggcgcggg tcacatccag ggcgcggcgt ggttgcccat tgggcttgct
     1861 ggtatcccag acaaactcac ctgtgtagct ggtcaggcgg gcaatcaggt gagccaggtc
     1921 tttgatgctg atctccatac ccgagcccaa gttaacaggc tcggcgctgt catagcgctc
     1981 ggctgccagc aggatgcctt cgcaggcgtc ctccacgtag aggaactctc gagtgggtga
     2041 gccgtcgccc caaagcacca cctgccggtc gccgcgttcc tgggcttcga taaatttacg
     2101 gattaaagcc gggatgacgt gcgaggtttg caagtcgaag ttgtcgcgcg ggccgtacag
     2161 gttgaccggc agcaggtaga tggcgttgaa gccatattgc tcgcggtaag cctgtgcctg
     2221 caccaacagc attttcttgg ctaggccata cggtgcgttg gtttcctctg ggtagccgtc
     2281 ccataagttc tcctcccgaa aaggcatggg agtgaatttt gggtaggcgc aaatggtgcc
     2341 aatggcgaca aacttatcga tatgacgaat ccaggcctca tgcatcaact gcacccccat
     2401 catcagattg atgtagaaaa aatcggcggg tctggaacgg tttgcgccga tgccgcctgc
     2461 tagggctgcc aggtggatca cgatggtttg aaggtttgag gctgttggta gaaggtaatc
     2521 atcgtacatg cggcggatgt cagcgatgtc caccaagtta tactgctcaa cccgcgggat
     2581 cagcacctcc gccgcgccgc gcgccttcaa cttttctacc aaaaaggagc ccaagaagcc
     2641 tgctccaccg gtaacgatta cacgtttatt taaccaataa tttgaagatt gaatgtcaga
     2701 catcagaatt tactccctcg tcacggatgc ggcgagcgct tgtgtaattt tcattgaacg
     2761 ataaattggg cattgcggta gcgcggattc tgcgggtctg ggatcagccc ggtgcgcaag
     2821 gcgtgcaaga cctcgcgcac gccatcatcc aaggtgtacg cggcttcgaa gcccagcttg
     2881 gactgcactt tctcaaacga gacggaaata tcgcgcatat ctccgccaaa agtcatatct
     2941 ttatatcgca ccacagtctc aggcaggcgt ttcaagatca gcgagacaac ctggtctttg
     3001 gtggcgttgc cgccttttgt gcccagattg tagacctgac ctcgaatatc tgcctcaggg
     3061 gctttcagac ccagcaagat gccgcgcacg gcatctcgaa tgtgcataaa ggaacgcgaa
     3121 tacccgcgct ggtaaatgag caactcgcgc cgggtatagg cctcgaggat aaactggttg
     3181 acgatcatgt cgaagcgggt acgaggagat attccataca acgtagccag tcggaagatc
     3241 agcggagcac aggggttgct cgcctgacca agtaggtagc gctcagcagc gatcttggtc
     3301 tctgcataaa gagactgagg gttaagcggt gtatcttcgg tcaccggatt cccatctggc
     3361 gagaggccgt agttgctgta ggtggaagcg tatataaatc gacctactcc aagctccaca
     3421 gcctgttcgt aaacttgctg ggtagcctcc acgttgtatc gccaagcgac ctgtctgccc
     3481 accgcctggc aggcggggaa accggcgatt gccgccaaat gcaccacagc tgctggtctg
     3541 gaccagtcgt cgcgcacggc attgcgcact gcacgtggct cccagatgtt ggtcttgacg
     3601 aaatgaaaat cgggatgcga catgtaggcc agcaaggatt cgccgccgaa gagcaggtca
     3661 tccagtacgg tgacgcgata accaacccgc aacaactccc cggtcagcag ggagccgata
     3721 tagcccgcac cgcctgtgac gaggatatgc cgttgagcag gttcgctgcc gaaatctagt
     3781 cgtgagctgc tagctttcat gcactttctc caggcgtaaa tgcactttca taccatgcag
     3841 ctcaatgacg ggcaggtcag catcttttcc atcgatcatc tctgccacgc tgatgccttg
     3901 ttcaagacag gtcaagcggc agacatcagc gatatcgcct tcaccgatga tatgcacgcg
     3961 gttgtaccca gcctttttga catctccgag cagctcgcgt acgtgttggc gagtgcgccg
     4021 gtacagcttc atcgatgtat cgatgtagtg gagagtaagg cgcgcccgga aggcgatgcc
     4081 ctcgggggtg atgatataac gaagcttgcg ccgctgggcg cgttttactt tgacatagcc
     4141 cttggctacc agacgcttca gataccagtt caccgtcccg actgccacgc ccaactgcga
     4201 tgccagtgag gcctgagtga tgtcaggatc atcctcaatg tgttctagca ggtttaattc
     4261 gtgaatggtt tcaggtggtg tttccatgat atctaaaatt cagccgctga attataacat
     4321 atgtcgggta tatacaaggc tcattcacca ggggtcaaga tgggcatctc cggtgcattc
     4381 cgcagattgt aaacacggac gtactccagg ttgttgatgc gcaccacgtg gtctggagtg
     4441 agttgctcaa aataagccat gaactctggg ctaggcaggc cgcgttgcca ttggttcagg
     4501 tagagcacga cgatatcggt cttgcgtaaa tcgtggaatg taaagtccag cccaattggg
     4561 acggtaaccc cgtcgtagaa atacgaaaag cagccatccc cgtaccagga aatcgcccgc
     4621 tccttgcctg tttggggcag cgaattcagg tagcgcgccg cttgatctaa gccttcaccc
     4681 cagccgacca tcatgacctg ggtagctgcc tttgagccac ctaagatggg gttaaagtag
     4741 ttcaaatagt acggaaagga ttggattaca ctccacattt gccccagtag gacgattccc
     4801 aagagcaatc cgcctgctaa acgacgagag attgtggaca gtctctttgc gtaacggttg
     4861 gctgccttta acaaggcaag ccagcccaag gcggcgatca agtccaacgg agcaaagatc
     4921 ggcagcaggt aacggtcaaa cttctttgct cccaggctca taaaaatggt gaagagacct
     4981 gtaaaaagca gcagcaccag gatcaggcgc gtgttttcca gcggaaatac ttttgggaaa
     5041 aacaaagcta acagaacaaa caccagccca accaagataa agggggtgat gcgccacaag
     5101 tacaccagcg ggtagaagta ccaagaggat atgccaccca gctgagtttc cccgtagaaa
     5161 tagctgggta tttcatgccc ttcgctggca tacgctgtgg cttgggagaa tacgctggtc
     5221 aggctaccca ccggatccac ccacatggca ggccagagca gaatgaatac cagccaagcc
     5281 actgcaaacc agagcaggaa tggtgagaaa gcttgccaca ggtgccgctt tcggaaaaga
     5341 tctcggatgg gggtgcgcct tccgctttgc agccattcga tcaacaaaag caagccaaag
     5401 aaaggcgcca gaaagaatgc tggggatttg gttagccagg ccagtccggc ggcgactgca
     5461 gagagaagca ggtaacccat ccggcgattc aggaacaagt aggccaaaaa tgaaagcaat
     5521 gagagcagca tgagggcgct gagcaagccg tcaaggtgta gcatgcgcga aagcgcaatc
     5581 gaaaatggat caaaggcgat caacaggaaa gccagaaagg ctggtagggt ccccagtagc
     5641 cggactgcga tcaagaatga cagtcccagg gcaagcacaa tgcccagcaa gacgaaggtt
     5701 cgactggcga ccagcaattc cagcgatgac cttccgtagc tgcgcaggaa tgtgtggaag
     5761 cgggtgggcg tcatgttgcc ttcgggcgag atcttaacat agcctggaaa acgccatagg
     5821 aagccagccg aacccgccca ggtgatggtt acaccggggt gctcgcgctg gaatgtgtct
     5881 ttgaaattac tatgcgccag tgcattgtaa aaattggctg agcgtagcaa ccacttgggt
     5941 tcatccacgg tgacaaagcg gtccaacgcc agcccgcgcg ttagccagag cattaataaa
     6001 acccccacaa aaatggccgt acccatccag ccgcggcgat tgacaaaggg gcgaagcaat
     6061 ctaatcactg gcgtacatca cctcttcatc ctttagaagg atgccagtgt cagccaagcg
     6121 ggatgcaggc aacggaggcg cgtcgtccat gttgtaaatt cgtgcgtatt cgatatcacc
     6181 aatgtggatg acgtgttcgg gggaaaactg ttcg
//
LOCUS       DBNGSX010000446         3403 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_734769, whole genome shotgun sequence.
ACCESSION   DBNGSX010000446 DBNGSX010000000
VERSION     DBNGSX010000446.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_734769"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            825..>3403
                     /locus_tag="AC16CB_13270"
     CDS             825..>3403
                     /locus_tag="AC16CB_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR3343484.1"
                     /translation="MTIEMIPDQDKITDLNDGNSYVLQSEEVIRRLATFSDKGLSSEE
                     AERRLKEFGPNALTEAPPPTFWQMLWEQFNNFVVIMLIVAALISGILGEFIEAAAIMA
                     IVVLNAALGVVQQRRAEQALAALRKLASPEAHVIRDGSRQVIPSPQLVPGDLVLLEAG
                     NYIPADIRLLEAVNLRIEEAALTGESVPVQKDATARLESGIPLGDRKNTAFMGTLVNY
                     GRGRGVVTDTGMRTQIGLIAEMLQSVQQEPTPLQRRLDQLGKTLGWGALTICALVFVV
                     GWARGFNPLDMFMIAVGLAIAAVPEGLPAVVTISLALGMREMIKRHALIRRLSSVETL
                     GSATTICSDKTGTLTQNEMTATRMWVDGKFIEITGSGYNPNGDFIIDGKAVNPTDYQG
                     ALTALWVATLNNDADLEITGASDGRQTYRMVGDPTEGALIVAAAKVGARTRELNEAYP
                     RVQEVPFDATRKRMVTVHALRHPKPEDISPFYDEKPHGYVVAVKGAPDIVLDLCSSYQ
                     HMGDYPTPLDESQRMRILSANDSLTQDALRVLGLAYKVIPEQPDIEDMQALEKDLIFV
                     GLIGMIDPPRLEVPPALDVADQAGIRTIMITGDYPNTARAIAESIGLLRPGHQVLTGS
                     EMNQMDDQTLQRQVKYTDVFARVSPEHKLRIVEALQANGEVVAMTGDGVNDAPSIKKA
                     DIGIAMGITGTDVAKESADMVLTDDNYASIVSAVEQGRIIYANIRKFVFFLLSSNVAE
                     IMIIFLATLAGLPTPLTVIQLLWLNLLTDGAPALALAMEKGDPDEMNRPPRPPSEPII
                     NKSMQLGIFIQTITQTGAVLTAFGLGLLWHLAQGDILPPGANPLTFLLGYDWRGLDVQ
                     TAET"
BASE COUNT          799 a          959 c          922 g          723 t
ORIGIN      
        1 atatcgcgtc cggtgccatc tccttcgacg aaggggataa tgggatgatc cggcacctgc
       61 agcaacccat ctcggatggt aattgtctct ccgcccactg gcgcttttat tgtattggaa
      121 ctcatgaaat gctctccctt gtatttagat aaccgcaaga caattaaacc acaaaggaca
      181 caagtacaca aagagtattt tgaaaatagc tttaacaaat aaaaccaacg taactacttg
      241 cccatcttcc ctttccagtg agaaagggaa gaagagagtc aaggttttca cctagatggt
      301 ttggattgat cgacattgaa aaactgtgag ttaaaagtga aaatagcttg ccaaccatgc
      361 aaatatttgt tatgattact tgtaggtggg gagtagctgc ctgccatcta ggtaggggaa
      421 ctgtcgtcat cacggaattt ttcccggcag ttcaattgct ttcctcgatt cagccagacc
      481 accatgacga tagttgtggt ggtttgtatc ttaatagggc tccaatgcac aggctttcgt
      541 tagatgactt tctcaataga gcacaacgca aaacaaagga gaagctatgt acatgcgatg
      601 gctaagccag ttcatgttat tgagactggt tgatttggtc ctcttggtgt tttttctcat
      661 cgccttgatc catcaaccca tacgaaagct catcattcgc ctccagaggt aaagttgggt
      721 atgaattttc aggttgccat ctcggcgcgc gctagaaata aagagaatta aggagtaggc
      781 gagcaatttg attttccgcc aaccccattc ggatagagtc aactatgaca attgaaatga
      841 ttccagatca agacaaaatt accgatctaa atgacggcaa ctcttacgta cttcaatccg
      901 aagaggttat ccggcgttta gcaacctttt cggataaagg gctatcatcg gaagaggcag
      961 agcgccgtct gaaagaattc ggccccaacg cactcaccga agccccgcca cccaccttct
     1021 ggcagatgct gtgggagcaa ttcaacaact tcgtcgtgat catgctgatc gtggcggcac
     1081 tcatctcggg catcttgggc gagttcatcg aagccgcggc gattatggca attgtagtgc
     1141 tgaatgccgc cctgggagtc gtccaacagc gccgtgctga gcaagctttg gcagctctgc
     1201 gcaagctggc ttcgcccgag gcgcacgtga tccgtgatgg ctcgcgccag gtgatcccat
     1261 cccctcaact ggttccaggt gacctggtgc tgctggaagc cggcaactat atccctgcag
     1321 atatccgctt gcttgaggca gtaaatctgc gtatcgagga ggctgccctg accggcgaat
     1381 ccgtccccgt ccagaaggat gctactgccc gccttgagtc gggtattccc ctgggagacc
     1441 gcaagaacac ggccttcatg ggcactctgg tcaactatgg gcgcgggcgc ggcgtcgtga
     1501 cggacacggg catgcgcacg cagattggcc tgatcgccga aatgctgcaa tcggtgcaac
     1561 aggaacccac gcctttgcag cgccggctag accagctggg gaaaaccctg ggctggggcg
     1621 ccctgaccat ctgcgccctg gtgtttgtag tgggctgggc gcgcggcttt aacccgctgg
     1681 atatgttcat gatcgcagta gggctggcga tcgccgcagt gcccgaaggc ttgcccgccg
     1741 tggtgaccat cagcctggcg ctgggcatgc gcgagatgat caagcgccac gccctaatcc
     1801 gtcgcctgtc gtcggtagag acgctgggct cggcgaccac aatctgctcc gacaaaaccg
     1861 gcacgctgac ccagaacgaa atgaccgcca ctcgcatgtg ggtggatgga aagtttattg
     1921 agatcaccgg cagcggctac aaccccaacg gcgatttcat tatcgatgga aaagcggtca
     1981 accccaccga ttaccagggg gcactgactg ctttgtgggt ggctacgctc aacaacgacg
     2041 cagatctcga aatcaccggt gccagcgacg gcaggcaaac ctatcgcatg gtgggagatc
     2101 ctactgaagg cgcgctgatt gttgcagcag ctaaggtcgg agcgcgcacg cgagagttga
     2161 acgaggctta tccacgcgtt caagaagtgc ccttcgacgc cacgcgcaag cgcatggtaa
     2221 ccgtgcacgc ccttcgtcac cccaagccgg aagatatctc tcccttctat gatgagaagc
     2281 cacatggata cgtggtggca gttaaaggcg cgccagacat tgtgctcgat ctatgctctt
     2341 cctaccagca tatgggtgat taccctaccc cactggacga gtcacaacgc atgcgcatcc
     2401 tgtcggccaa tgacagcctg acacaggacg ccctgcgcgt gctaggcctt gcctataaag
     2461 tcatcccgga gcagcccgat atcgaagaca tgcaggccct ggagaaagac ttgatattcg
     2521 tcgggctgat cggcatgatc gatccgccac gcctcgaggt gccgccagcg ctcgacgtag
     2581 ccgaccaggc aggtatccgc acgatcatga tcaccggcga ttaccccaat accgctcgcg
     2641 ccatcgccga atcgatcggc ttgctgcgcc ccgggcacca ggtgctcacc ggcagcgaga
     2701 tgaaccagat ggacgatcag acacttcagc gccaggtgaa atacactgac gtatttgcac
     2761 gtgtctctcc agagcacaag ctgcgcatcg tggaagctct ccaggcgaat ggcgaagttg
     2821 tcgccatgac cggcgacggg gtgaatgacg ccccttcgat caagaaggcc gacatcggca
     2881 tagccatggg catcaccggc acagatgttg ccaaggaaag cgccgacatg gtgttgacgg
     2941 atgacaacta cgccagcatc gtctcggcag tggagcaggg acggatcatc tacgccaaca
     3001 tccgtaagtt tgtgttcttc ttgctctcat cgaacgtggc cgagatcatg atcatcttcc
     3061 tggctaccct ggcaggattg cccaccccac tgactgttat ccaactgctg tggctgaacc
     3121 tgctcactga tggcgctccg gccctggcgc tggcgatgga aaaaggcgat ccagatgaga
     3181 tgaaccgccc gccaaggcca cccagcgagc cgatcatcaa caagagcatg cagctaggca
     3241 tcttcatcca gaccatcacc caaaccggcg ccgtgctaac agcttttggc ctgggcttgt
     3301 tgtggcacct ggcccagggg gatatcctgc caccaggcgc caacccgctg acctttctgc
     3361 tgggttacga ttggcgcgga ttggacgtgc aaaccgcgga gac
//
LOCUS       DBNGSX010000447         3037 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_803966, whole genome shotgun sequence.
ACCESSION   DBNGSX010000447 DBNGSX010000000
VERSION     DBNGSX010000447.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_803966"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="AC16CB_13275"
     CDS             <1..763
                     /locus_tag="AC16CB_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017870816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HIR3343485.1"
                     /translation="IPHIEIVRIGSRVPVFLPMRVTDELCDMLQKYHPLWLNIHVNHP
                     NEISAELNQACDKLTRAGIPLGNQAVLLAGVNDNVHIQRQLVQELVRMRVRPYYLYQC
                     DLVEGAGHFRTAIAKGIEIMEGLRGHTSGYAVHQYIIDAPGGGGKIPVTPNYLLSMSD
                     HKIILRNYEGYITTYEEPVDYLPSQAATFKGEKRPEPGQAGLTGLLEGEQMFIKPEGF
                     DELHDRGGSQHRLKDARKWVPLGIGSGEESEESES"
     gene            complement(919..2199)
                     /locus_tag="AC16CB_13280"
     CDS             complement(919..2199)
                     /locus_tag="AC16CB_13280"
                     /inference="COORDINATES: protein motif:HMM:NF020130.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HIR3343486.1"
                     /translation="MNDQLDKPEDSQETISFFDRPGVQVQILPIGPGIPLGIEQVSSP
                     SLGIGGAYGTWGESYDNASLPSLVEHRLGAPLDESDRLDLSPLGFDRRHHITDLTDEE
                     HYQVEIQVGAQLLRAAAQACGWEPSEVDAVMIGLSGPIAEDYTEQIARTAGVPESALK
                     VSIHKACDGSVGALHLALNPDLPTHRLKGRNIAEELYGKKVLVGGIEGLSRFIRKTRD
                     KNAMQLFGNGAGVIGLIPGKTMKFLVGKTHEVFDDEGVLAVRMYYPHSRHTEEGGSMV
                     EVTQTGEHHIRIAGLMHEPEDASPVVMAGPMGMVKLFVRSGVDVVRDVYAAYQARLNE
                     LGMAGKQVATTVVHHANLKINRLKEKHLQKEGINLPMPWVIKEFGNVSAASNMIAFLR
                     QLPGLRPGDHVLFDGFGAGTYYDVLAVVLSETAA"
     gene            2625..>3037
                     /locus_tag="AC16CB_13285"
     CDS             2625..>3037
                     /locus_tag="AC16CB_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011430072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR3343487.1"
                     /translation="MSIQVDYSGTQIVSPSVLSGLEATVGNTPLLPLRRVSAEISPRV
                     QVLAKAEWFNPGGSIKDRPALSILRSAKEEGLLRPGVSLLDSTSGNMGIAYATFGAAL
                     GVPVTLVLPANASPERQTILRALGAELILTDPLEGS"
BASE COUNT          652 a          813 c          828 g          744 t
ORIGIN      
        1 gatccctcac atagagattg tgcgcattgg ctcgcgtgtg ccagtgtttc tccccatgcg
       61 ggtcaccgac gagctgtgcg acatgctcca gaagtaccac ccgctatggt tgaatatcca
      121 tgtcaaccac cccaatgaga tcagtgctga gctgaatcag gcttgcgaca agttgacccg
      181 cgccggcatc cctttgggga atcaggctgt gctgctggct ggcgtgaacg acaatgtcca
      241 tatccagcga caactggtgc aagagctggt gcgcatgcgc gtccgcccct attacctgta
      301 ccagtgcgat ctggtagagg gtgcgggtca cttccgcacc gcaatcgcca aaggaattga
      361 gattatggaa ggcttgcgcg gtcacacgtc gggttacgcc gtacaccagt acatcattga
      421 tgccccgggt ggcggcggaa agatcccggt gacgcccaat tacttgttga gcatgtctga
      481 ccacaagatt attctgcgca attacgaagg ctacattacc acttacgaag aaccagtgga
      541 ttatctcccc agccaggctg ccacgttcaa aggtgagaag cgccccgagc caggtcaggc
      601 tggactcacc gggttgcttg aaggcgagca gatgttcatt aaacccgagg gtttcgacga
      661 attgcatgac cgcggtggta gccaacatcg tttgaaagac gcccgcaaat gggtgccctt
      721 agggattggt tcaggggaag aatcggagga atctgagtct tgagtttctg cgtttagcgt
      781 caaaaattct cctcgccccc gagagcgagg agaatggcgc tcaaacgggt gtgcgggtgc
      841 attgcacccg cacaccattt ttttatggga attatagggg gtctcgcgat aggcaagaaa
      901 gcggttgcca ggtaatgttt atgctgcagt ctcacttaga acgaccgcta acacatcgta
      961 gtatgtgcct gctccaaaac catcgaatag cacgtggtcg cctggacgca accctgggag
     1021 ctggcgcagg aaggcgatca tgttggatgc ggcactaaca ttgccgaatt ctttgatgac
     1081 ccagggcata ggcaggttaa tgccctcttt ttgaagatgt ttttccttca gacggttgat
     1141 cttcaggttg gcgtggtgca caactgtggt ggcgacctgc ttgccagcca tgccaagctc
     1201 gttcaagcgc gcctggtaag cggcatagac gtctcgcacc acgtcaacgc cggaacgcac
     1261 gaatagcttg accatgccca tcgggcccgc catgactact ggtgaggcat cctcgggctc
     1321 gtgcatcaga ccggcaatac ggatgtggtg ctcgcctgtc tgggtgactt cgaccatcga
     1381 tccgccctct tctgtgtggc gagaatgagg gtagtacatg cgcacagcca gcacgccctc
     1441 gtcgtcgaat acttcgtggg tcttgcccac caggaatttc atcgtctttc ccggaattaa
     1501 accaatcacc ccggcgccgt tgccgaataa ctgcatggca tttttatccc gcgtcttgcg
     1561 tatgaagcgg ctcagtccct caataccacc taccagcact ttcttgccgt agagttcttc
     1621 agcgatattg cgccccttaa gccggtgggt tggcaggtct ggattaagcg ccagatgcag
     1681 ggcgcccaca gacccgtcgc aggctttgtg aatgctcacc tttaatgcgc tctcgggcac
     1741 gccggccgtg cgggcaattt gttcggtgta atcctctgct atcggtccgg aaagcccaat
     1801 catcacggca tctacttcgc ttggctccca gccacaagct tgggcggcgg cacgaagcag
     1861 ttgggcaccc acctggattt caacctggta atgttcttcg tcggttagat ctgtgatgtg
     1921 gtgccggcgg tcaaatccca acggcgatag atccaggcgg tcggattcgt cgaggggtgc
     1981 tcctaggcgg tgttccacta ggcttggcaa gctggcatta tcataactct ctccccaggt
     2041 tccgtatgcg ccgccaatac ccagtgacgg ggaagagacc tgctcgatgc ccagcggtat
     2101 gccaggccct atcggcagga tttgcacttg tactccaggg cgatcaaaaa aggaaatagt
     2161 ctcctgtgaa tcctctggct tgtctaattg gtcattcatg gtttgtacct gttctttcca
     2221 aattaataaa tagcaccatc cgacaagaag ctggcgggtg gaatcgtact gcggataagt
     2281 tacttcgtta tgtcttggct atattagtta aacctggtcg ttttgtcaag acagcaattt
     2341 tcaactattg acaatttggt ccttgacaaa ctgcttgagt tgcatataat tattactaac
     2401 tttctcataa aaatcgtttt tggagagatt tgattgatgg tttcttgcat gtgtctgggg
     2461 cggggtatgt aaccactgcc tcgtcacaat aaccccggaa aattccggca gccagcagat
     2521 tgacgacagg tggttgcgac cccgcatacg cagaaagatt tgcgtggcag agtgtccact
     2581 gcctgttttg ttatttgttg gctgttttat ttgttggaga gtagatgagc attcaagttg
     2641 attatagcgg tacccaaatc gtttctccct ccgtcctatc aggcctggag gctactgtgg
     2701 gtaacacgcc actcctgcct ctgcgccgcg tttcagcgga gatatcaccg cgcgttcagg
     2761 tgctcgcaaa agccgaatgg ttcaacccgg gtggctcgat taaagaccgc ccggcgctca
     2821 gtatcctgcg ctctgctaag gaagaaggct tgttaagacc gggtgtgagc ttactcgatt
     2881 ccacttctgg aaatatgggc atcgcttacg ctacttttgg agccgcgctc ggtgtaccgg
     2941 tcaccctggt gctgcctgcc aatgccagcc cagaacggca gactatcttg cgtgccctgg
     3001 gagcggaact gatcctgact gaccccttgg aaggctc
//
LOCUS       DBNGSX010000448         7350 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1081186, whole genome shotgun sequence.
ACCESSION   DBNGSX010000448 DBNGSX010000000
VERSION     DBNGSX010000448.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7350)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1081186"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="AC16CB_13290"
     CDS             <1..655
                     /locus_tag="AC16CB_13290"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HIR3343488.1"
                     /translation="YNWCPNGLPMVIAIDEIFMPCYIDDYKRLEATLRYNFQSNITAI
                     FFLIAGVGWIWLSRSSPDDLSADGKIAPRVGFLAPDFSLLDRYSNQITLSELRGRPVI
                     INLWASWCIPCREEMPALEQVHQAYKDQGLIVLAVNATNQDNQQAAIRFSDNLGLTFP
                     ILFDTQGSVSQAYRLQSLPTTYFVDQNGIIQEVVVGGPMAEALLRVRVEALLEDAYP"
     gene            652..1392
                     /locus_tag="AC16CB_13295"
     CDS             652..1392
                     /locus_tag="AC16CB_13295"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR3343489.1"
                     /translation="MMPVLQIGPIALPMPALIVLLGIWLGLALAERFAARNRLTGDYI
                     FNLALTGLVVGLVAARLGYVLRYPYAFSENIMDVFSRNLGLFDTQTGFVVGLIASAIY
                     AQRKQISLKATLDALTPAFAVFSIALSLSHIASGDAYGSPATLPWSIQLWGAQRHPSQ
                     IYETLAGIAILGYLWPSHKHLANLKPGEYFLKFVIMNSAARLFLEAFRGDSAVTLGGY
                     RAAQIISWLILAVCLYAIKRLRTTQGPP"
     gene            1402..1812
                     /locus_tag="AC16CB_13300"
     CDS             1402..1812
                     /locus_tag="AC16CB_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HIR3343490.1"
                     /translation="MSSQLSFYIPLNISYDEALEAVVKALSAEGFGILTRIDVKATLK
                     EKIGVEFRPYTILGACNPPLAHRALSSDPETGMLLPCNVTVETSEAGGSIIRIVNPQT
                     LLSIGSLSEDPELAAVAKEAHTRLERVAQTLKQT"
     gene            1809..2534
                     /locus_tag="AC16CB_13305"
     CDS             1809..2534
                     /locus_tag="AC16CB_13305"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3343491.1"
                     /translation="MSLSGKHVLITGSARRVGRILALSVAQAGGIVFIHHAHSPEDAE
                     NVRQEIIDLGGQAYILQADLLDPSMAQKLIDQASTYGPLYALVNNAAIFEPLSWETTD
                     LESWNRHLALNLSAPFLLSQAFARSHTELTVGRIVNILDWRALRPGPDHFPYTISKAA
                     LVALTRSLAIALSPQITVNGIALGAILPPTHPVDLRKVIQHVPAGRWADPEEVGDALI
                     FLLEGPSYITGEIIHLDGGRHLV"
     gene            2610..2966
                     /locus_tag="AC16CB_13310"
     CDS             2610..2966
                     /locus_tag="AC16CB_13310"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIR3343492.1"
                     /translation="MDKIIIKDLLARGVIGVNEWERQVTQDILMNIVIFSDVRKAGKT
                     DDLTYSVDYRKIAKQAVVLAETAKRLTVEALASDIASMCLEDAGVLKVQVRVEKPGAI
                     RFARSVGVEIERSRDG"
     gene            2959..3444
                     /gene="folK"
                     /locus_tag="AC16CB_13315"
     CDS             2959..3444
                     /gene="folK"
                     /locus_tag="AC16CB_13315"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013456.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIR3343493.1"
                     /translation="MDEHQYILGLGSNIDPVTNLHRTVEMLRQEVTVDNISTIWVTKP
                     VGEAGPDFLNAAVQIKSTLEPNTLKSQVLRPIEAKLGRIRTKQKNSPRTIDIDILFAD
                     DQIVDDEVWEYTHKALPLAELKPDLIHPTTGETLEILARRLLRSTPLDHRPAILKLNK
                     I"
     gene            complement(3486..4118)
                     /gene="folE"
                     /locus_tag="AC16CB_13320"
     CDS             complement(3486..4118)
                     /gene="folE"
                     /locus_tag="AC16CB_13320"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019010402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HIR3343494.1"
                     /translation="MDTQFELLYKIADLRQDGEIDHDSIKDAVIQLLQAIGEDPDREG
                     LIRTPERVSRMYAELLDGYRMDPIEIVNDALFEVMYDEMVLVRDIEFYSLCEHHMLPF
                     FGRAHVAYLPKGRVLGLSKIPRLVDMFAHRLQVQERMTRQIAEFLDELLHPAGVAVVV
                     EGLHLCTAMRGVKKHDARMTTSAMLGYFRKNIATRQEFLDNISRGAAPMF"
     gene            4302..4748
                     /locus_tag="AC16CB_13325"
     CDS             4302..4748
                     /locus_tag="AC16CB_13325"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR3343495.1"
                     /translation="MELTNILRKAELFNGLSEDELQHVASLCGERRLARGEILTTQGV
                     RGDELFIVTEGLVEVLITESQKDSHKIIVNLGKGQLIGEMSLVDKGPRSATVRAITEP
                     TLVQVIQRDQFEALCEQNTHIGYIVMRNIATDLSFKLRHRNLSEGK"
     gene            4752..4961
                     /locus_tag="AC16CB_13330"
     CDS             4752..4961
                     /locus_tag="AC16CB_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343496.1"
                     /translation="MTVYKVSYVVTGSEHPGAIVNLDHAPAVGEHITLGDHKFEVIEV
                     LNLVPPRGDFHYIHATIKLVATFQD"
     gene            complement(5063..5635)
                     /gene="hpf"
                     /locus_tag="AC16CB_13335"
     CDS             complement(5063..5635)
                     /gene="hpf"
                     /locus_tag="AC16CB_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIR3343497.1"
                     /translation="MSMDVQVYGRNMDVTDRIRTYVSKKISKLDRYLSGIEEARVDVA
                     YVKSARSASDRQVAQITVRGKGFILRSEERSDDIITALDIALDKMQRRIERYKGKRYR
                     GRGDGRSAAEVVPQVVEEIEEESGELGPMIARRKRFTLHPMDEMEALEQMQLLGHENF
                     FVFYNANTHSVNVLYKRRDGNYGLIEPDVG"
     gene            complement(5748..6473)
                     /locus_tag="AC16CB_13340"
     CDS             complement(5748..6473)
                     /locus_tag="AC16CB_13340"
                     /inference="COORDINATES: protein motif:HMM:NF012383.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIR3343498.1"
                     /translation="MAKTYEAGRPAYRLYQWLWTGLDWLLPPHCGGCGKEGTRWCANC
                     QRETALINPPICARCGNSQQAEGTCARCKEHPPIYSVLRSWAFYSGPLRNSIHRLKYN
                     HDIALGDLLARPLIDCLEVSGWSINIIVPVPISLSRKAERGYNQAALLARPIALRFGV
                     NYCPKALIKTRETRSQVGLNVEERRKNVHGAFKSDQQVVAGKRVLVVDDVTTSGATLN
                     ACADALRTAGAMEIYCLTLARAL"
     gene            complement(6466..>7350)
                     /locus_tag="AC16CB_13345"
     CDS             complement(6466..>7350)
                     /locus_tag="AC16CB_13345"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR3343499.1"
                     /translation="MRESTGEDPLQSRLADYAARGETATLEEIELSQPISERVILPLA
                     RKAGEIALRFTPQNALQSTQRKIELAGNPRGLEPSLFWILRFITALAVGSLMWYLMFV
                     GVKNWQWGTKILVIFGFLILGFFLPEMFLISKIQRRQKEIRKAMPDALDLLTICVEAG
                     LGFDGAMQKVYEKWDNELSLAFGRVLKEIQLGKLRREALRDMSDRMGVGEMTSFVAAV
                     TQSEQLGVSMAKVLRIQSDQMRIKRRQLAEEEAHKAPIKMLIPMAILIFPSICIVLMT
                     PAVLMLLRSALKSVLFGG"
BASE COUNT         1962 a         1843 c         1623 g         1922 t
ORIGIN      
        1 ctacaactgg tgtccgaatg gtcttccaat ggtcatcgct attgatgaga tctttatgcc
       61 atgttatatt gatgactata agcgtttgga ggcaacgttg cgttataact tccaatcgaa
      121 tataaccgct atattctttc tgatcgcagg agtaggctgg atttggctga gtagatcatc
      181 ccccgatgat ttatcggcag atggcaagat tgcgccgcgt gtgggctttc tggcaccgga
      241 ttttagcctt ctggatcgat atagtaatca aataacttta tcggaacttc gcggccggcc
      301 agtgatcatt aacctatggg caagttggtg cattccatgc cgcgaggaaa tgcctgccct
      361 cgaacaagta catcaagcat ataaagacca aggtctgatc gtactggcag taaatgccac
      421 caatcaagac aaccagcaag cagccattcg tttctctgac aatcttggac tcactttccc
      481 gattcttttc gacacgcagg gcagtgtctc ccaggcttac cgtctgcaat ccctaccaac
      541 cacctatttt gtagatcaaa atgggatcat ccaggaagtt gtggttggcg gcccaatggc
      601 agaagcatta ctccgagtgc gtgtagaagc actcctggag gatgcttacc catgatgccg
      661 gtattgcaga tcggaccaat tgctctgcct atgcccgctt taattgtcct actggggata
      721 tggctggggc tcgcgcttgc tgaacgtttt gccgcacgca atcgtttaac tggcgactac
      781 attttcaact tggctctcac aggcttagtt gttggcttgg ttgcggcgcg tttgggatat
      841 gttttacgct atccgtatgc tttttcagaa aatattatgg atgttttttc tcgcaatctg
      901 ggattgtttg atacacaaac tggctttgtc gtggggctga tcgcatccgc gatctatgca
      961 caacgcaagc aaatttcatt gaaagccacg cttgatgcac ttactccggc atttgctgtt
     1021 ttcagcattg cactcagcct ttcccacata gcttcaggag atgcttatgg ctcgccagca
     1081 actttacctt ggagcattca attatgggga gctcaacgcc atccgagcca aatttacgaa
     1141 acgctcgctg gaatcgccat actaggatat ctgtggccat cccacaaaca tctcgcaaac
     1201 ctaaaacccg gtgaatattt ccttaaattc gttataatga actccgcagc tcgattattt
     1261 ctagaagcct ttcgtggcga cagcgccgtt accctgggcg ggtatcgagc tgcgcagatc
     1321 attagttggc taatcctggc cgtttgtcta tacgcaatca agcgcttaag aactactcaa
     1381 ggtccaccat aaggagaaca catgtcttct cagcttagct tttatattcc cctaaatatc
     1441 tcatatgacg aagctctcga ggcggttgtc aaagcgctaa gtgcagaagg atttggtatc
     1501 ctgactcgca ttgatgtcaa ggcgacattg aaggaaaaga tcggcgtcga gtttcgccca
     1561 tatacaatac taggagcttg caacccccca ttagcccacc gagctctctc atccgatccc
     1621 gagacaggga tgctacttcc gtgcaatgtc accgtagaaa ccagcgaagc gggtgggtct
     1681 attatccgca tcgtgaatcc ccaaactctg ctatcaatcg ggtcgctctc tgaggacccc
     1741 gaattagccg cagtcgccaa agaagctcac actcgcctgg agcgcgtcgc ccaaacactt
     1801 aaacagacgt gagtctatct ggcaagcatg ttttaattac cggttcagcc cgccgtgttg
     1861 ggcgtatcct ggctctatca gttgcgcaag ctggagggat tgtattcatc catcatgctc
     1921 actctcctga agatgccgaa aatgtacgcc aggagattat cgacctgggt ggacaggcat
     1981 atatactaca ggcggacctg cttgatccgt caatggcaca gaaattaatt gatcaagcga
     2041 gtacctacgg tccgttatat gctttggtga ataatgcggc catctttgaa ccactttcgt
     2101 gggagaccac agatcttgaa tcctggaacc gacacctggc cctcaattta tccgccccat
     2161 ttctcctcag ccaggctttt gcacgctcgc acacggagct gaccgtaggt cgtattgtca
     2221 atatcttaga ttggcgcgcc ctgcgccctg ggccagacca cttcccatac accatcagca
     2281 aagcagcctt agtagccctc actcgatccc tagcaatagc cttatccccg cagatcactg
     2341 tgaacgggat tgctttaggc gcgatactcc cccccactca tccagttgac ttaagaaaag
     2401 tcatccagca tgtacccgct ggtcgatggg cagacccaga ggaagtcggt gacgcgctaa
     2461 tttttcttct ggaaggaccg agctacatca cgggtgaaat tatccacttg gatggtgggc
     2521 ggcacctcgt atagagcaca tactttctac caacggaaaa tctgactaag tatcagagcc
     2581 acttttcgtg aaaccaggaa ggcgtcatta tggataagat aatcataaaa gacctgttag
     2641 ctcggggtgt gattggtgtg aatgagtggg agcgccaagt cacccaggat atactgatga
     2701 acatcgtaat attcagcgac gtacgcaagg cgggcaaaac cgacgatctt acctatagcg
     2761 tagattatcg aaaaattgcc aagcaggccg ttgttctagc tgagactgct aaacggttga
     2821 ccgttgaagc tctggcttca gacatcgcaa gtatgtgtct agaagatgct ggtgtattga
     2881 aagttcaagt tcgggtagaa aagccaggcg cgatacgttt tgcacgctca gtgggcgttg
     2941 aaattgaacg tagtagagat ggatgagcat caatatatct taggtctcgg gtccaatatt
     3001 gatccagtaa cgaacctgca tcgaactgta gaaatgctcc gtcaagaggt aaccgtcgat
     3061 aatatttcca ccatttgggt gaccaaacca gttggcgagg cagggccaga ttttcttaat
     3121 gcagctgttc aaataaaatc taccctggaa cctaatacat taaagtcgca ggtcctccgc
     3181 cctatcgaag ccaagctagg gcggataaga acaaaacaaa aaaattcccc ccgcacaatc
     3241 gacatagata ttctgttcgc agatgatcaa attgttgatg atgaggtgtg ggaatataca
     3301 cacaaagccc ttcccctggc tgaattgaaa cctgacctga tccatccaac tacaggggaa
     3361 actttggaaa tcttggctcg caggttattg cgctccacgc cactcgatca ccgtccggca
     3421 attcttaaac tcaataagat ttaaatctca attggggaac tcttcagcca atttatagat
     3481 ggggactaaa acattggcgc agccccgcgt gatatattat ccaagaactc ctgtcgagtg
     3541 gctatatttt tacgaaaata acctaacata gccgaggttg tcatgcgggc atcgtgtttc
     3601 ttaaccccac gcattgctgt acaaagatga agaccttcaa caactaccgc gacccctgct
     3661 ggatggagca attcgtctaa aaattcagca atctgccggg tcattcgctc ttgaacctgt
     3721 aacctgtggg cgaacatgtc taccaatcga ggtatttttg acagacccaa tactcttccc
     3781 tttggcaagt aagctacatg tgctcttcca aaaaatggaa gcatatggtg ctcgcagaga
     3841 ctatagaatt cgatgtcgcg aaccagcacc atttcatcat acatcacttc aaacaaagca
     3901 tcattgacaa tttcgatggg gtccatacgg taaccatcta gtaactcagc atacatgcgg
     3961 gatacccgct caggtgtgcg aattaaaccc tcccggtcgg gatcttcccc aattgcttgt
     4021 aacaattgaa ttaccgcgtc cttaattgaa tcatggtcta tttctccatc ctgacgcaga
     4081 tcagcaatct tatacagcag ctcaaattgt gtatccataa tgatccttag cattcttata
     4141 taattttgcg cctagtgtaa cacctctcat cgccaagcgt caacgcggag gcatacaaag
     4201 gaaactgcat gaagtagagc ctttctctac aaacaaaaac gaaaatgttg cagttaagaa
     4261 aaattcgtta taaaatgatt ctatcagtga gaggcagatt gatggaactg acaaacatcc
     4321 tgcgtaaggc agaacttttt aacggacttt cggaagacga gctccaacac gtcgcatcat
     4381 tatgtgggga gagacgcttg gcgcgaggtg agattcttac aacacaaggg gttcggggcg
     4441 acgagctatt tatcgtcacc gaaggtttgg ttgaggtgct catcacagag agtcaaaagg
     4501 attctcacaa aatcattgtc aacctgggta aggggcagct catcggcgaa atgtcattgg
     4561 ttgataaagg gcctcgctcg gcaactgtcc gcgccattac tgaacctact ttagttcagg
     4621 ttatccagag ggatcagttt gaagctttat gtgagcagaa tacccacatc ggatatattg
     4681 tgatgcgaaa tattgccaca gatctctctt tcaaactacg tcaccgcaat ctgagtgaag
     4741 gaaagtaggt gatgaccgtc tataaagtta gctatgtggt cacgggtagt gagcaccctg
     4801 gagccattgt gaatctcgac catgctcccg cagttggcga acacatcacc ctgggggatc
     4861 acaaatttga ggttatcgaa gtgttgaatc ttgtgccccc gcgtggcgat tttcattaca
     4921 ttcacgctac aatcaagcta gtagcaactt tccaagattg atttcccaaa gaagaattac
     4981 taaaatcaat cccccttcta ctcggaaggg ggattttgaa atattgagat ttgctcaaac
     5041 tttggctaga tattgcatac aatcagccaa catcgggttc gatcaggcca taattgccat
     5101 ctcgcctttt ataaagcaca ttgaccgaat gggtattcgc attgtagaaa acgaagaagt
     5161 tctcgtgtcc tagcaattgc atttgctcaa gggcttccat ctcatccatg gggtggagag
     5221 taaatcgctt cctgcgggca atcatcggcc ccaattcacc actttcttct tcgatctctt
     5281 caactacctg gggaacaacc tcagcggcac tcctcccgtc accacgccct ctgtagcgct
     5341 tgcctttata tcgctcaatc ctgcgttgca ttttatccaa agcgatatca agagcggtga
     5401 tgatgtcgtc agaacgctct tccgagcgca atataaatcc tttgccgcgg actgtaattt
     5461 gtgccacttg tcgatcagac gcgctgcgag ccgatttcac ataagcgacg tcaacccttg
     5521 cctcctcaat cccagataag tatcgatcta atttcgaaat ctttttacta acataagttc
     5581 ggatgcgatc cgtgacatcc atattcctac cataaacttg tacatccatg gacatttttt
     5641 cctcctatat catcgtataa aatgccattc aatgctcgcc actaaataaa ccttataata
     5701 actgaaacat atcataagaa tggttagcag ttgtactcca tatctaatta taaagcccgc
     5761 gccagtgtaa ggcaataaat ttccatagct ccagcagtcc tcaaagcgtc ggcacaagcg
     5821 ttcaaggtag ccccactcgt cgttacgtcg tccacaacaa ggactcgctt cccagctacc
     5881 acctgctgat cagatttgaa tgctccatga acgttcttgc gtcgctcttc cacattgaga
     5941 cctacctgag agcgcgtctc tctggttttg attaaggcct ttggacaata attgacacca
     6001 aacctcaatg ctatcggtcg agccaatagt gctgcttgat tatatcctcg ctcagccttc
     6061 ctggaaagac tgattggaac aggtacaatg atattaatac tccagcccga aacttcaagg
     6121 caatcaatta gcgggcgcgc taataaatca cccagtgcaa tgtcatgatt gtacttcaag
     6181 cgatgaatgg aatttcgtaa gggaccagaa taaaatgccc aagatctcaa cacagaataa
     6241 attggaggat gttccttgca tctagcgcag gttccctcag cttgctggga gtttccgcaa
     6301 cgagcacaaa ttggagggtt gattagtgcg gtttcgcgct gacaattagc acaccagcgc
     6361 gtaccttctt tcccacaccc cccgcaatga ggcggtaata gccagtccag ccctgtccac
     6421 agccattggt agagacggta agcagggcgt cctgcttcgt atgtcttagc caccaaataa
     6481 cacagatttt aatgccgaac gcaataacat gagaactgcc ggcgtcatca atacaatgca
     6541 aatagatgga aagattaata tagccatagg aattagcatc ttgattggcg ccttatgggc
     6601 ttcttcttca gccaactggc gtctttttat tcgcatctgg tccgattgga tgcgcaacac
     6661 ctttgccatg ctaacgccaa gctgctcact ttgggtaact gcagcaacga aactggtcat
     6721 ttcacccaca cccatgcgat cagacatgtc ccgcagcgct tctcgtcgca atttacccag
     6781 ttggatctcc ttaagtaccc gaccaaaggc taacgatagt tcattatccc atttctcata
     6841 aactttttgc attgccccat caaagcctag acccgcttcc acgcagattg ttaataaatc
     6901 aagggcatct ggcatggctt tgcgaatctc tttctggcgt ctctggattt tgctaattag
     6961 aaacatctcc ggtaagaaga agcctagtat caaaaagcca aagataacga gaattttcgt
     7021 cccccactgc cagtttttaa ctcccacaaa catcagatac cacatcaaag atccgacggc
     7081 aagcgcagtg ataaagcgaa gtatccaaaa tagggatggc tcaaggcctc gtggattacc
     7141 agcgagctct atcttgcgtt gggttgattg aagagcattt tgaggggtaa atcgcaaggc
     7201 aatttcacca gctttgcgtg ccaggggtag tatgacgcgt tctgagattg gttgtgataa
     7261 ctcaatctcc tccaaagtag ctgtttcgcc tcgtgcggca taatctgcta aacgtgattg
     7321 cagcgggtct tctccggttg attcacgcat
//
LOCUS       DBNGSX010000449         3880 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1842543, whole genome shotgun sequence.
ACCESSION   DBNGSX010000449 DBNGSX010000000
VERSION     DBNGSX010000449.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1842543"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="AC16CB_13350"
     CDS             <1..288
                     /locus_tag="AC16CB_13350"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase family protein"
                     /protein_id="HIR3343500.1"
                     /translation="PDCRYTEPWLEKIGVQCPQDGGEIVERKTRKGRVFYGCANYPNC
                     DFTSWKRPLPAPCPNCQGLLVVADRRNAQCLNCAEQYPLDQISADELVGSA"
     gene            440..1450
                     /locus_tag="AC16CB_13355"
     CDS             440..1450
                     /locus_tag="AC16CB_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343501.1"
                     /translation="MDAIREQIKKPLVLGIAGLVVGIALGILYGWVINPVEWTDAAVE
                     HLRADLQQEYLRMSIDSYAQNRDAELAKKRYADLGPNAAEVMGAVMESPGAQNFETIL
                     AFSATVQGGVEPAIPPEGEAAQPEEEKQGSNILRMAAVVLFLIFVVIAGGAFYLFIWR
                     PRMGESVPVTPAMQAQEAAQQAEWTDYSAEGGETPIVQFMSSYKFGDDLFDESFAIDS
                     PAGDFMGECGVGISETIGVGEPKKVTTFEVWLFDKNDIQTVTKVLMSANAFLDEPTRQ
                     RLSAKGEPILAEPGIETVLETETLILVARVVDMGYGEGAVPDSSFFDRLLLELAVWPK
                     EG"
     gene            complement(1551..2018)
                     /gene="greA"
                     /locus_tag="AC16CB_13360"
     CDS             complement(1551..2018)
                     /gene="greA"
                     /locus_tag="AC16CB_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIR3343502.1"
                     /translation="MDDQIYLTPEGAARLKIELEQLKVTGRDEIAQRLRSAVQMGDLS
                     ENADYHKAKEDQGFLEGRIQELEYLLKNATIIEKNGARPEVADIGTHVTIQEGSEPPE
                     TYHLVGAKEADPRNGRISNESPIGRALLGHRAGDEVVVETPNGKIHLRIITIE"
     gene            complement(2064..3215)
                     /locus_tag="AC16CB_13365"
     CDS             complement(2064..3215)
                     /locus_tag="AC16CB_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HIR3343503.1"
                     /translation="MFYTRQHLEALEDQTLAPYACRSKDSKGRAYPEDEPEYRTAFQR
                     DRDRILHTTAFRRLEYKTQVFINYEGDYYRTRLTHTLEVSQIGRTIARTLGANEDLLE
                     AICLAHDLGHPPFGHSGETILARLMQEHGGFDHNKQSLRIITQLEGRYPDFPGLNLTW
                     EVREGIVKHESEYDTADASEYNPDLRGHLEAQIANVADELAYTTHDLDDGLRSGMITP
                     HMLEGITLWEILAESVGWSSPVLDDLTRHRLIRRLIGLEVTDLVNATHARLQQSNVKS
                     VKDLQSLSYNVISFSEDMHRRNRQLKDFLYNHLYRHHRVVRMAVKAERIISDLFHAYC
                     SEPAILPGHIQEFIEERGLERTICDYIAGMTDRFAMDEHQKLFEPSTKP"
     gene            complement(3289..>3880)
                     /locus_tag="AC16CB_13370"
     CDS             complement(3289..>3880)
                     /locus_tag="AC16CB_13370"
                     /inference="COORDINATES: protein motif:HMM:NF012899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid kinase family protein"
                     /protein_id="HIR3343504.1"
                     /translation="RGFAEVWHEAVALNRIVIDALHDAGLPAVTFSPSACVLTRDGQV
                     VNWDLATMRAALQAGLIPVVYGDVIFDTARGGTILSTEALFEHLARHLGPERVLLAGL
                     EEGVWADFPACTQLVHEITPESLPQLIPVLGGSNATDVTGGMADKVQQSLALVQELPG
                     LEVLIFSGEKQHQVERALLGERVGTLVRANIPQETI"
BASE COUNT          852 a          963 c         1113 g          952 t
ORIGIN      
        1 ccggattgcc gttataccga gccatggctg gagaagattg gggtgcaatg cccacaggat
       61 ggaggcgaga ttgtagagcg aaagactcgc aagggtcgcg tattctatgg gtgtgccaat
      121 tacccgaact gcgacttcac ttcttggaag cgacccttac ctgctccatg cccaaattgc
      181 cagggtttgt tagtagtcgc cgatcggcgt aatgcccagt gcctgaactg cgccgagcaa
      241 tacccgttag accagatttc tgcagacgag ttggttgggt cggcataaga cttgcaacaa
      301 accggatgag cttctccact tatgaaagct catgatagaa tatgtgtgct aaacattatt
      361 cgcagaacgt tgcataaatc aagggtttcg tctagaatta gagacaacga acccggaatc
      421 atggtatttg gagagagata tggacgccat tcgtgagcag atcaaaaagc ctctcgtctt
      481 gggcattgct ggcttggttg tgggcattgc cttgggtatc ttgtacggct gggtgatcaa
      541 cccggtggag tggacggatg ccgctgttga gcacttacgc gccgacctgc agcaagaata
      601 cctgcgcatg tctatcgatt catatgccca aaatcgcgat gcagagctgg caaaaaagcg
      661 ctacgcggac ctgggaccca atgcagccga ggtgatgggt gccgtgatgg aatccccggg
      721 cgcgcagaat ttcgagacca tcctggcttt cagtgcgacg gttcaaggcg gggttgagcc
      781 tgcaattcca ccagagggcg aggctgcaca gcctgaagaa gagaagcaag gcagcaatat
      841 cctcagaatg gcggcagtcg tcctattctt gatatttgtc gtgattgctg gtggggcttt
      901 ttatctattc atctggcgtc cgagaatggg cgaaagcgtc cccgtcacac ctgctatgca
      961 ggcgcaggaa gccgcccagc aagccgagtg gactgattat tcagctgagg gtggcgagac
     1021 gcccatcgtg cagtttatgt catcttataa gttcggggat gatctcttcg atgaatcctt
     1081 tgccatcgat tcgcccgctg gagattttat gggcgagtgt ggtgttggta tctcagagac
     1141 catcggtgtg ggtgagccga agaaggtaac caccttcgag gtttggttgt tcgataagaa
     1201 tgatatccag acggttacca aggtgctgat gagcgccaat gcctttttag atgaacccac
     1261 ccgccagcgc ctgtcagcca aaggcgaacc aattttggct gagccaggga tcgaaacggt
     1321 gctggaaacc gagacattga tactagtggc ccgcgttgta gatatgggat atggtgaagg
     1381 cgctgtgcct gatagcagct tctttgaccg attattgctg gagttagcgg tctggcctaa
     1441 agaaggttaa aacatcaggt tcagcggtag gtataatatc tcgctgaacc ctttatgcac
     1501 atttgacatt gggttttacg ggcgctacgc acccgtaaaa cccactgttt ttattcgatt
     1561 gttataatgc gcaagtgaat tttaccattt ggcgtttcta cgactacttc gtcgccagca
     1621 cgatgcccca ggagagccct accaatgggg gattcgtttg agatgcgacc gttgcgcgga
     1681 tcggcctctt tggcacctac caggtggtaa gtctcaggag gttcagaacc ctcttggatg
     1741 gtcacgtgcg tgccgatatc cgctacctca ggtcgggcgc catttttttc gataatcgtg
     1801 gcatttttta acaggtactc taactcctgg atgcgccctt ccaggaagcc ctggtcctct
     1861 ttggctttgt gataatctgc attctcggag agatcgccca tttggaccgc ggagcgcagg
     1921 cgttgagcaa tctcgtcacg acctgtcact tttagctgct ccagttcgat tttgaggcga
     1981 gctgctcctt cgggggtaag gtaaatttga tcatccatgc tttaaccttc tactttcaag
     2041 tatcacttaa catcctaccg atattaaggt ttcgtggacg gttcgaatag cttttgatgc
     2101 tcgtccatgg cgaagcggtc ggtcatgccg gcaatgtaat cgcagatggt gcgttccaat
     2161 cctcgctcct caatgaactc ctggatgtga cctggcagga tggcaggttc gctgcagtag
     2221 gcatggaaca agtcgctgat gatccgttca gccttcaccg ccatgcgcac caccctgtga
     2281 tgccggtata ggtgattata caggaagtct ttgagctggc ggttgcgccg gtgcatatct
     2341 tcgctgaagc taatgacatt atatgatagg ctttgcagat ctttgaccga tttgacgttg
     2401 ctctgttgca ggcgtgcgtg cgtggcgttg acaagatcgg tcacttctaa gccaatcaag
     2461 cgccggatca ggcgatggcg cgtcaggtca tcgagaacgg gggaactcca gcccacgctc
     2521 tcagctaaga tctcccaaag tgtgatgcct tccagcatat ggggggtgat catgccggag
     2581 cgtaacccat catccaggtc gtgcgtggta taggctagct cgtcagctac attggcgatc
     2641 tgtgcctcca ggtggccgcg cagatctggg ttgtactcgc tggcatcggc agtgtcgtat
     2701 tcagactcgt gcttgacaat gccctcgcgc acttcccagg tcaggttgag gcctggaaag
     2761 tcagggtagc gcccttccag ttgggtgatg atacgcaggg actgcttgtt gtggtcaaaa
     2821 ccaccatgct cttgcattaa ccgagctaag atagtctctc ccgagtgacc aaaaggcggg
     2881 tgccctagat catgggctaa gcaaatcgcc tctaagaggt cctcgttagc acccaaggtg
     2941 cgagcgatgg tgcgcccgat ttgggacacc tccagagtgt gcgtcagacg cgtgcggtaa
     3001 tagtcgccct cataattgat gaagacttgg gtcttatatt ccaggcgacg gaaagctgtg
     3061 gtgtgcaaaa tacggtcccg gtcgcgctgg aaggccgtgc gatattccgg ttcatcttcc
     3121 gggtaggcgc ggccttttga gtccttgctg cggcatgcat aaggtgccag agtttggtct
     3181 tctagggctt cgaggtgctg gcgagtatag aacattggga ttagatcgca gggtttagaa
     3241 cgtaaagaat agataattgg ccattcacgc caagtgtgta attataatct atattgtttc
     3301 ttgggggatg ttcgcccgca cgagcgtgcc cactcgctcg cccagcaagg ctctttcaac
     3361 ctgatgttgt ttctcgccag agaaaatgag gacttccagt ccaggtaact cttgaaccaa
     3421 tgctaggctc tgctgtacct tgtcggccat gcccccagtg acatcggtag cgtttgaacc
     3481 acccagtacg ggtattagct ggggcaggct ttcgggtgtg atttcgtgga ctagttgtgt
     3541 gcaggcgggg aaatcagccc acaccccctc ttctaatcca gcgagcaaga cgcgttcagg
     3601 acccagatgg cgggccaggt gctcgaagag ggcctcggtc gagagaatgg tgccgccgcg
     3661 ggcagtgtcg aatatcacgt cgccatacac gacagggata agaccggctt gcaaggcagc
     3721 tcgcatggtc gccagatccc aattgaccac ctgtccatca cgggttaaga cacaggctga
     3781 aggggagaat gtaaccgcag gcaggcctgc atcgtgcagt gcgtcaatca cgatgcggtt
     3841 gagtgcaacg gcctcgtgcc acacttcggc gaagccgcgc
//
LOCUS       DBNGSX010000450         5298 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1357800, whole genome shotgun sequence.
ACCESSION   DBNGSX010000450 DBNGSX010000000
VERSION     DBNGSX010000450.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1357800"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..906
                     /locus_tag="AC16CB_13375"
     CDS             157..906
                     /locus_tag="AC16CB_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3343505.1"
                     /translation="MLEIILVEDNQRLRKALKSGLEATSQARQVFDSDRGEAALEYCL
                     EAQPEPSKTVILMDVQLAGEMNGIQAAVAIRREFPRFPVVFYSIQDEDVYYRDFRRSG
                     ILSHYAYVRKSNYLLPEMILPLLQDAIAGRSFIDAEIEARVQEVRLKDENDPMALLEP
                     NEQVVARLLAEGLTNEQIAARLGFRDKRTISRTNGQIYVAWGLTNSTTDEKVARTRAV
                     LIVQSGQLLSWDEDGVAHVLNEQGDWIPWVQ"
     gene            917..3037
                     /locus_tag="AC16CB_13380"
     CDS             917..3037
                     /locus_tag="AC16CB_13380"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3343506.1"
                     /translation="MSGNFYLDWAIQAISLFNTILLLWLGLTVLLNAERRTPGLWIAG
                     AQLLMGAAFFLSHSIILGYGQFVFGPGVNFWWRVGWIPVIGVPYTWYGVMLWYAGYWS
                     EPVSELRRRHRYWFMITTGLVALVFGLLIFANPLPTFSQVAELKLNATPSIGGIPLLI
                     LIYPLYIATCISLSIDVLRRPAPSGRLMGDLARRRARPWLFAASFVQLGVSILVGLVM
                     LGVILSAGRRVYDHTMAVTVAWFDLVIAGLITSAIVLVGQAIVSYEVFTGKPLPRGGL
                     RRYWQRLIILAAGYAVAVSFSLTLPLKPIYSLMLSTALMIAFYALLTWRSYNERESYI
                     ERLRPFVASQRLYEQITASSLQSPPELDAYPPFQALCADLLGAQCAYLIPLGPLAPLA
                     GPPLVYPQAKDAPLPGLGEIIPQWTNPQDLCTPLDPGVFYGAAWGIPLWSERGLVGGL
                     LLGEKVDGSLYTQEDFEIARAVCERLVDTQASAEMARRLMALQRQRLAESQVLDRQTR
                     RALHDDVLPRLHAMMLDLSGGKLPPHTTDEMMKSLGELHHQISDLLRELPPATAPQIT
                     RLGLIEALRQVVDGELKGAYDQVEWHIPSDADARARDLPPYKAEVLFYAAREALRNAA
                     KHAGWQQAAAPLYLRIRARWHDGFDLSIEDNGRGVGGSGDAQRGSGQGLTLHSTLMAV
                     IGGSLEMESTPGVSTCVILRIPDL"
     gene            complement(3065..4198)
                     /locus_tag="AC16CB_13385"
     CDS             complement(3065..4198)
                     /locus_tag="AC16CB_13385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3343507.1"
                     /translation="MDTNPVFTGRLGLQQRVLPAYRAPFFNALATACEGGLSVFAGQP
                     TLGEAIQTTEKLEAARFYPARNLHFGRVGSPFYLCWQGGLIEWLDAWQPDALIVEANP
                     RYLSTRLACSWMRARHRPVLGWGLGAPQGKGSLEPFRKPARARFLSHFDGMIAYSQRG
                     AEEYRAYGFPAENVFVAPNAAAPRPATPPQERPQSFGERPVVLFVGRLQRRKRIDNLL
                     HACAALPLESQPEVWIVGDGPAMDDFQTLARQVYPRAQFPGAKRAADLEPHFSRADLF
                     VLPGTGGLAVQEAMAHGLPVIVAEGDGTQDDLVRPENGWRVPPGDLEALTRTLQDALT
                     NASRLRHMGRQSYRIVAEEANLERMVEVFVNALSSMKIGYRGD"
     gene            complement(4250..5104)
                     /locus_tag="AC16CB_13390"
     CDS             complement(4250..5104)
                     /locus_tag="AC16CB_13390"
                     /inference="COORDINATES: protein motif:HMM:NF016908.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HIR3343508.1"
                     /translation="MSNPLLSIAALAARLIPMPLKRALYRSGPLARLIRSGLNRAAPR
                     GLTRVQVAGGELQDVSLLLDLQEEKDYWLGTYEPDLQAAILQFAQPGMVAYDVGANIG
                     YISLLLAKAVGESGQVYAFEALPANIERLRANLALNGLETRVQVVEAAVTNQSAPTQF
                     LIGPSGGMGKAAGSAGRQGEYDQSITINGLALDDFVFGQSHPLPHIIKIDIEGGEVLA
                     LPGMRRLLRQARPFLLLELHGAEAAQVAWDELTNSGYLICRMETGYPLLNSAAEMDWK
                     AYVIAKPE"
BASE COUNT         1066 a         1553 c         1550 g         1129 t
ORIGIN      
        1 gccatcttga tcgtgctcat ttataaacaa ttccgatatc gggggaaaaa ttgggccaac
       61 cacctgaaat cagtatttag cttggggatg accggttacg tggtttgggt catatttctg
      121 atagtcgcca tcccgttact gttcaggtaa gggcgtatgc ttgaaatcat ccttgtcgaa
      181 gataatcaac gcctgcgcaa agccttaaaa tccgggctgg aggccaccag tcaggcgcgc
      241 caggttttcg atagcgaccg cggcgaagct gccctggagt actgcctgga agcgcagcca
      301 gagccatcta aaaccgtcat cttgatggat gtgcagttgg ctggggagat gaacggcatc
      361 caggcagcag tcgccatccg gcgagagttt cctcgctttc cagtcgtttt ttattcgatt
      421 caggacgagg atgtctacta ccgtgatttt cgccgctctg gtatcctcag ccactacgct
      481 tatgtgcgca aatccaacta cctgctccct gagatgatcc ttccgctgct gcaagatgcc
      541 atcgccgggc gcagctttat cgatgcggag atcgaggcgc gcgtccagga ggtgcgtttg
      601 aaagacgaaa acgacccgat ggcgttgctg gagccaaacg agcaggtcgt cgctcgtctg
      661 ctggcggagg ggttgaccaa cgaacaaatc gccgcccgtc tgggtttccg cgataagcgc
      721 actatcagcc gcaccaacgg gcagatatat gtcgcctggg ggttgaccaa ttcgaccacc
      781 gatgagaagg tggcgcgcac ccgggcagtg ctgatcgtcc aatcaggcca gctgttaagc
      841 tgggacgaag atggcgtcgc ccatgtgctg aacgagcagg gcgattggat tccttgggtg
      901 cagtgatcgg cgaaagatga gcggtaactt ttatctcgac tgggcaatcc aggccatctc
      961 actgtttaac accatcctgc tgctctggct gggtctcacg gtgctgctca atgccgagcg
     1021 acgcacgccg ggactgtgga ttgctggggc acagctgctc atgggtgctg ccttcttcct
     1081 gagccattct atcatcctgg gatatggaca atttgttttt ggtccaggcg tcaatttttg
     1141 gtggcgggtg ggttggatac cggtgatagg cgtgccatat acctggtacg gagtcatgtt
     1201 atggtacgct ggctactgga gcgaaccggt ctcggagttg cgccgccgcc atcgctactg
     1261 gtttatgatc accaccgggc tggtggctct ggtctttggg ctgctgatct ttgccaaccc
     1321 cctgcctact ttcagccagg tggcagagct gaaattaaat gccaccccgt ctattggtgg
     1381 aatcccgtta ttaatcctga tctacccgtt gtatatcgcc acctgtatca gcctctcgat
     1441 tgatgtgctg cgccgcccgg ctccatccgg tcgtttgatg ggggatttgg cgcgccgccg
     1501 agcccgccca tggttgttcg cagcttcgtt tgtgcaactt ggggtcagca tcctggtagg
     1561 gctggtgatg ctgggggtta ttttgagcgc cggtcgacgg gtgtacgacc acactatggc
     1621 agtcactgtg gcctggttcg acctggtcat cgcaggcctg atcaccagcg ccatcgttct
     1681 cgttggacag gcgattgtct cctacgaagt ttttaccggc aaacctctac cccgcggcgg
     1741 cttgcgacgc tactggcagc ggttgattat cctggcggcc gggtacgccg tggcggtcag
     1801 tttcagcctg accctacccc tgaaacccat ctacagcctg atgctgagca ctgctctaat
     1861 gatcgctttc tacgccttgc tcacctggcg atcttacaac gagcgagaga gttacatcga
     1921 gcgcctgcga ccctttgtcg ccagccaaag gctgtatgag caaataactg cttcctctct
     1981 ccagtcgccg ccagaattgg atgcttatcc cccgtttcag gcgctatgcg ccgatctatt
     2041 gggggcgcag tgcgcgtacc tcatccctct cggcccgcta gcaccgctgg caggcccacc
     2101 gttggtatac ccgcaagcaa aagatgcccc gctacctggt ctgggtgaaa tcatccccca
     2161 atggaccaat ccgcaggacc tctgtacccc actcgatccg ggagtatttt acggggctgc
     2221 ctggggcatc ccactgtgga gcgagcgcgg cctggtaggc gggctgctgt tgggtgaaaa
     2281 agtcgacggg tcgctgtata cgcaggagga tttcgagatc gcccgcgccg tctgcgaacg
     2341 gctggtagat acccaggcca gcgcagagat ggcgcgccgc ttgatggcgc tgcaacgcca
     2401 gcgccttgcg gagagccagg tgctcgacag gcaaactcgc cgcgccttgc acgatgatgt
     2461 cttgccccgc ctgcacgcca tgatgctgga tttgagcggg gggaagctgc ctccacacac
     2521 caccgatgag atgatgaaat ccttggggga actccaccac cagatttctg acctgctgcg
     2581 cgagctgcct cctgccactg ccccgcaaat cacgcgtctg gggctgatcg aggcgctgcg
     2641 ccaggtggta gatggcgagt tgaaaggtgc ctatgaccag gtcgaatggc acatcccgtc
     2701 ggatgctgat gcgcgcgctc gtgatttacc cccatataaa gcagaagtgc tcttttacgc
     2761 ggcgcgggag gccttgcgta acgctgccaa acatgctgga tggcaacagg ccgctgcgcc
     2821 actatacctg cgcatccgtg caaggtggca cgatggtttc gatctgagta ttgaagacaa
     2881 tggacggggg gtgggtgggt caggcgacgc ccagcgcggc agcggtcaag gtctcaccct
     2941 gcatagcacg ctgatggctg tgatcggcgg gtcactggaa atggagagca cgccgggcgt
     3001 atccacctgt gtgatcctga gaatccccga tctttaaaca agatttatgg ggagtatcta
     3061 cgcactaatc cccacgataa ccaattttca tacttgatag cgcgttgaca aatacttcca
     3121 ccatgcgttc caggttggct tcttcagcca cgatgcggta agattggcga cccatgtggc
     3181 gcaggcggga ggcattggtg agggcatctt gcagcgtgcg ggtcagtgcc tcgaggtcgc
     3241 ccggcggcac tcgccagccg ttctcggggc gcaccaggtc atcctgcgta ccgtctccct
     3301 cggcgacgat caccggcagt ccgtgtgcca tggcctcctg caccgctagc cctccggtgc
     3361 cgggaagcac aaacaggtcg gcgcgcgaaa aatgcggctc cagatcggca gcgcgcttgg
     3421 ctccgggaaa ttgagcacgg gggtaaacct ggcgagctag ggtttggaaa tcatccatcg
     3481 ctggcccgtc tcccacgatc cacacctccg gttgcgactc gagcggcaag gcagcgcagg
     3541 cgtgaagcag gttgtcgatg cgcttgcgcc tctgcaggcg gcctacaaac agcaccactg
     3601 gtcgctcgcc aaaagattgc gggcgctcct gcggcggggt ggcggggcgc ggcgcggcgg
     3661 cattgggcgc cacgaagaca ttctccgcgg gaaagccata cgcccggtat tcttctgcgc
     3721 cgcgctggct gtaagcgatc atgccatcga aatgggatag gaaacgagcg cgagcgggct
     3781 tgcggaatgg ctccagcgag cccttgcctt gcggcgctcc tagcccccac cccaacaccg
     3841 ggcgatggcg ggcacgcatc caggaacatg ccaggcgtgt ggagagatag cgcgggttgg
     3901 cctcaacgat cagggcatcg ggttgccatg cgtccagcca ctcgatcaga cctccctgcc
     3961 agcataagta gaaaggcgag cctacccgcc caaaatgcaa attgcgagcc gggtaaaacc
     4021 gggctgcctc caatttttcc gttgtctgga tggcctcgcc tagggtgggc tgaccagcaa
     4081 agacgctcaa accgccttcg caggcggtgg ctagtgcatt gaaaaatgga gcgcgataag
     4141 ctggtagcac gcgctgttgt agaccaagac gacctgtgaa gacaggattt gtatccatcc
     4201 ccctgattat acaggatggt cactgcctgc ggatgccata gattaccgcc tattcgggtt
     4261 tcgcgataac atacgctttc caatccattt cggcagcaga attcagaagg ggataaccgg
     4321 tctccatgcg gcaaataagg tacccgctgt ttgtcagctc atcccaggct acttgagctg
     4381 cttcagcgcc atgcagctcc aacaggagaa acgggcgagc ctgcctcaac aaccggcgca
     4441 tgcccggcag ggccagtacc tcgccaccct ctatatctat cttgatgatg tgcggcaggg
     4501 ggtgactttg cccaaacaca aaatcatcca acgccagccc gttaatggtg atggactgat
     4561 catattcgcc ttgccgccct gccgatccgg cggctttgcc cattccgccc gaaggaccga
     4621 tcagaaattg agtcggggca gactgattcg tcaccgcagc ctcaacaact tgaactctgg
     4681 tctcgagccc gttgagtgcc aggttggcgc gcaagcgttc gatattggcg ggcaaagctt
     4741 caaaagcata cacttgccca ctctcaccca cggcttttgc caggagcaag gagatgtagc
     4801 caatattggc acctacatcg tacgccacca tcccaggttg tgcaaactgc aggatggcag
     4861 cttgcagatc cggctcgtac gttcccagcc agtaatcctt ctcttcttgt agatccaaca
     4921 gcaagctcac atcctgcaat tcgccgcctg ccacctggac acgcgtcagg ccgcgcggtg
     4981 cagcccggtt taatccggag cggatcaatc tcgccagcgg gccagatcgg tagagcgccc
     5041 tttttagagg catcgggatc aagcgggctg ctagagcagc tattgagagt aggggatttg
     5101 acatagacag cgttagctag ccgctaattc tccactaaga catccaaata tctctgtacc
     5161 agatcatcca acccgaaagc ttgttcggcg cgcgcccgag ccgcgcgccg gaagtggccc
     5221 tgattctcta gaatctccaa ggcagccaca ctgagggcag agatgtccgg tcgctcaagc
     5281 tgccaggggt ctccaccg
//
LOCUS       DBNGSX010000451        11430 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_804564, whole genome shotgun sequence.
ACCESSION   DBNGSX010000451 DBNGSX010000000
VERSION     DBNGSX010000451.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11430)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11430)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11430
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_804564"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      7..125
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            170..559
                     /gene="gcvH"
                     /locus_tag="AC16CB_13395"
     CDS             170..559
                     /gene="gcvH"
                     /locus_tag="AC16CB_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HIR3343509.1"
                     /translation="MNIPSDLKYTANDEWIRIEGNIGTVGITDYAQDQLSDIVFVEVI
                     VSAGEKVKKGDACATIESVKAAADVYMPASGKIFEVNDSLPDTPEVVNNDPYGAAWMV
                     KFEINDPSELDQLLDSTAYEKNIAEKE"
     gene            625..1968
                     /gene="gcvPA"
                     /locus_tag="AC16CB_13400"
     CDS             625..1968
                     /gene="gcvPA"
                     /locus_tag="AC16CB_13400"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HIR3343510.1"
                     /translation="MFFPHTDEDRKSMLETIGVQRIEDLFNAVPGAHRFPDLKLPPAL
                     SEMEAMAGMQDLAWSNENARDLVCFLGAGAYNHYIPAAVDMLLRRGEFYTAYTPYQAE
                     ISQGTLQAIFDYQSLIAALTGMEVSNASHYDGATATAEAITMAQANFRGKRTKFVLSP
                     ALHPHYRQTVHTYNHGSELIFVGEDVDVTASPESLMPLIDENTALVMVQYPDFFGRIY
                     DYTALAEATHNAGALFAVAVNPLSLGLLKPPGEFGADIVVGEGQPLGIPLSYGGPYLG
                     IFATRKEYVRKMAGRLVGETVDNRGQRAYVLTLTAREQHIRREKATSNICTNQGLIAL
                     ASAIYMALLGKHGLRQVAELCYHKAHYAARKLGAIRGYQLWNQTPFFHEFVLRCPSST
                     EEINAHLLDHGILGGYDLGQAYPKLKNHLLIAVTEMNSKEDIDLLAEVLSEVSHG"
     gene            1961..3427
                     /gene="gcvPB"
                     /locus_tag="AC16CB_13405"
     CDS             1961..3427
                     /gene="gcvPB"
                     /locus_tag="AC16CB_13405"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HIR3343511.1"
                     /translation="MGEPLLCEMSVPGRVGFRYPAPDVPMEPLPEALVRSNLPLPELY
                     EVDVVRHFTRISQMNHSIDTGFYPLGSCTMKYNPKVNEETARLPGFAHVHPLQPVETV
                     QGSLALMYDLQNWLKEIGGFAGVTLQPAAGAQGELTGVLILRAYHEKRGQTQRNKILI
                     PDSAHGTNPATSAMSGMRVVEIKSDARGNVDLEALRLECDDTLAGLMLTNPNTLGLFD
                     ENVEEVIRMVHEAGGLVYGDGANMNALLGIVRPGDLGFDVLHYNLHKTFSTPHGGGGP
                     GSGPVGVAAHLVDFLPGPVVGIIEEENEDEDMPPLYGLITPPHSIGRVKSFHGHFGMM
                     VRAYTYMLMHGKEGLRLIADYAVLNANYLLARLRDTYKLPYDRVCMHEFVLEGRWPDA
                     PDIHALDIAKRLMDFRFHPPTNYFPLIVHEALMIEPTETESKQTLDAFADALIKIAEE
                     AHSEPDLLKTAPHITPVGRVDEVKAAKELVLCCTVDIS"
     gene            3693..5426
                     /locus_tag="AC16CB_13410"
     CDS             3693..5426
                     /locus_tag="AC16CB_13410"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase family protein"
                     /protein_id="HIR3343512.1"
                     /translation="MSKSESLKVQFIAPSPTVEVHLENGKTICGPRGTPIGEFLKLLE
                     GESPAPIVGAVVNSELRELTYPIHVESHVRPVTMGEADGARIYRRSIVFLLESAFDYC
                     FPGAVLTIDHSVASGGYYCQVSERPALTEEELAGMEARMRQLVKADLAFQRKEVPLKE
                     AIDYFALKGAGDKVQLLKHREKDYLTLYQLQNFFDYHHGYMVPSTGYLKWFSLIPTGD
                     GFTLRFPRRHAPTVILPLPDYPKLLATFRQYGDWLQRLGISSVGALNDAIQAGRSREV
                     ILVSEALHEQQIAEIAAHIAQRRDQLRVVLVAGPSSSGKTTFSRRLSVQLLARGISPF
                     PLEMDNYFVDRDQTPLDENGELDFESIGALNHERLSEDLLRLSNGELVQIPDFNFKTG
                     KSTPGEEIQLKSNEIIILEGIHGLNPALISGIPSENAYRIYVSALTQLNLDRHNRVST
                     TDTRLIRRIVRDARERGYTAQQTIQRWDSVRRGEKRYIFPYQENADVMFNSALVYEVS
                     VLKPLVEPLLRQVPYGKPEYIEAKRLLAFVQWFLPLESDLIPDNSIMREFIGESILRD
                     FTLWHNHSETK"
     gene            5399..6769
                     /locus_tag="AC16CB_13415"
     CDS             5399..6769
                     /locus_tag="AC16CB_13415"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HIR3343513.1"
                     /translation="MAQPLGNQVNRDKLMRTVRVRNDQQLGTLGRLLTTVAEERGDIG
                     EIRLVQEGHRANIRDITIFVDDEAQLERVLKAMAGNPGTRILAVRDEVLELHQKGKIA
                     IRSRYQIDSLYTLRRVYTPGVANVCLKIAAEPSLARQYTAISHLVAIVTDGTAILGLG
                     DIGPLAGMPVMEGKAMLMETLVGLSGVPILLNTKDVDTIVKTVVSIAPTFAAIQLEDI
                     SAPRCFEIERRLQEQLDIPVMHDDQHGTAVVTTAALFNACRKTGMNLKEAVIGQIGLG
                     AAGNAIGRMLMALTGNSILGADISEDALQRFEQIGGLRSNLSEVMDRANIIIATTGVP
                     GLIKPSQVRQGQIILALSNPNPEIDPDAAIAAGAAFAADGKSVNNVLGFPGILRGAID
                     TYAPRITDEMYLAAAQTIAELTPREELVPNPLDKQVHQAVARAVAQKAIEQGLARAEF
                     VPYLEE"
     gene            6771..8510
                     /locus_tag="AC16CB_13420"
     CDS             6771..8510
                     /locus_tag="AC16CB_13420"
                     /inference="COORDINATES: protein motif:HMM:NF012471.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HIR3343514.1"
                     /translation="MHSSRVLGIFAVFLVALFLALVILTGFSQVSATEQTATGQAHLF
                     LAPTIAPNDGPWVVVAYFSNREQVNRVAERIEPWEVNYDQGFMLLEVDKDQYQWLLEL
                     GLQVVIAQELTELLHRPQFALPEQEAGIPGYPCYRTVEETYATGQQIAIDHPGLATWI
                     DIGDSWKKFAFGSDSGHDIMVLRLTNAQVSGSKPKLFILSSIHAREYAPAELNTRFAE
                     YLVNNYGTDPDATWLLDHHEIHLLLNGNPDGRERAEAGKYWRKNTDNNYCSDTDSRGA
                     DLNRNFEFLWGCCNGSSDRQCDITYRGPAPASEPETQAIQNYVRSIYPDQREDALSSP
                     APADASGVFIDLHSYGELVLWPWGFTYDAPPNHEALRTLGRKFAYFNGYTPEQSIELY
                     PTDGTTDDFAYGELGIAGYTFELGTAFFQSCSTFEGDILSDNLAALLYAAKIVRTPYQ
                     TPAGPDSMAVNISPKFAVKGQEVILSATIDDTLYSGGELTQSIVAAEYYIDVPPWVSS
                     TTPVSFAMSAQDGGFDEPSEPVMASVDTTGLASGRHILFVRGLDADGNWGAFTATFLW
                     IEEDQVYLPIIVK"
     gene            8541..9389
                     /locus_tag="AC16CB_13425"
     CDS             8541..9389
                     /locus_tag="AC16CB_13425"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR3343515.1"
                     /translation="MHKPANLDPIDYLVIGHLTCDLTPDGYRLGGTAAYSTLTAHALG
                     LRVGVVTSWGEEIPLGPLRHIPIANFPTDKSTIFENIHTPEGRVQVIHNVAPNLGFNL
                     VPDPWRNPAIVHLGPLVQEVEPSLIRNFPSALIGVTPQGFLRGWDAKGHVHFSEWPES
                     TFVLQRAGAAVISVEDVEGDEDRIAEMASSCRILAVTEREQGARLYWNGDVRRFRPPQ
                     IQFVDDTGAGDIFAAAFFVRLYTTRDPWEAARFATYLASFSVTRSGLDAIPTQEEIQE
                     CMVEVL"
     gene            9390..10184
                     /locus_tag="AC16CB_13430"
     CDS             9390..10184
                     /locus_tag="AC16CB_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR3343516.1"
                     /translation="MARTYTLVNQKGGVGKTTTAINLGAYLAYFGQRVLLVDLDPQAN
                     ATSCLRIDKHNVRGGTYEALIGSSPASGHILHNPRLKLSILPSTPELAGAEVELVPEL
                     ARETRLKKALDPIIDRYDYVLIDCPPSLGLLTLNGIVSAQNGVIIPVQCEYLALEGLS
                     QLTQTLGRVRNALFPQLSIRGVLLTMFDGRTNLANDVVKEVRRFFPDQVFETVIPRSV
                     RLAEAPSYGQPISYYAPSSPGAVAYAALAREVLERDGVHIPIPEEI"
     gene            10190..11041
                     /locus_tag="AC16CB_13435"
     CDS             10190..11041
                     /locus_tag="AC16CB_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR3343517.1"
                     /translation="MPRRPGLGRGLEALIPESEQPTSTGTSYIPQNRIVPNPRQPRSK
                     IDPQELAELSASIKEHGVLQPLLVSYDNLSGNYTLIAGERRLLAARQAGLETVPAIVR
                     EVNEQQRLELALIENLQRADLTPLEAAEAYRQLVEEYSLSHENIADRVGKSRAAVTNT
                     LRLLKLPIRVQQALADGRISEGHARALLALPTPEAQEAALRTILKQDLNVRQTEEIVR
                     KLSGLRPVSTPKANPSPELTALEERLRTKLGTRVKLNRSRKGGTLVIHFYSDEELDAL
                     LETLLQE"
     gene            11038..>11430
                     /locus_tag="AC16CB_13440"
     CDS             11038..>11430
                     /locus_tag="AC16CB_13440"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3343518.1"
                     /translation="MTRVLICANIYTLNPQQPHATAIVIDGERILRLGARQEILTEFA
                     GNGHRVEILDARHQTLIPGLIDAHFHLEQYALAQQMVDCETPTRAECERRVAERAQQM
                     PPGEWVLGHGWNQNDWPEGFGNNTDLDAV"
BASE COUNT         2771 a         3114 c         2886 g         2659 t
ORIGIN      
        1 ctgatgctgg aaccctctgg aaagtctggc aggttatcac aacccgcagt acgctagcct
       61 gcacaccgac ggggcaagcg cgacacccag cgcgaatctc tcaggttcaa gacagaggcg
      121 cacgcaatct acgcgtgcgc cttttattat ttttctccag gagactcaca tgaacatccc
      181 cagcgatttg aaatacactg ccaatgacga atggatcaga attgaaggca atatcggcac
      241 cgttggcatc accgattatg cccaggatca gctctcggac attgtttttg tcgaggttat
      301 cgttagcgca ggtgaaaaag taaagaaagg tgatgcttgc gctaccatcg agtcggtgaa
      361 agctgctgcc gatgtgtata tgccagccag cggaaagata ttcgaggtga atgactctct
      421 tccagatacc ccagaagttg tcaacaacga cccatatggc gctgcttgga tggtgaagtt
      481 cgagattaat gatcctagtg agctggatca gttgctcgat tcaaccgcat acgaaaagaa
      541 tatcgcggaa aaagaataat aagtgcatgg tgcgggctac agattaatcc tccaccctcg
      601 ccatggattg acaaaggagt tcgtatgttc tttccccata ctgatgaaga tcgcaaatcc
      661 atgctggaaa cgataggtgt gcagcgcatt gaggatcttt tcaatgcagt tcccggtgct
      721 catcgcttcc cggatttgaa actacctcct gccctatcgg agatggaggc tatggccggg
      781 atgcaagacc tggcatggtc gaatgagaat gcccgtgacc tggtgtgttt tcttggggct
      841 ggggcgtata atcactacat ccctgccgcc gtggatatgc tcctgcgacg cggggagttc
      901 tatacagcct acacacccta ccaggcagaa atctcacagg gcactctcca agccatattt
      961 gactatcaaa gcttaatcgc tgcactgacc ggcatggaag tatccaacgc ctcccattat
     1021 gacggcgcta ctgcgaccgc cgaggcaatc accatggcac aagctaactt tcgcggcaaa
     1081 cggaccaagt ttgtcctctc accagccttg catccgcact accgccagac ggtgcacacc
     1141 tacaatcatg gatctgaatt gatttttgtg ggggaggatg ttgatgtcac agctagtccg
     1201 gagagcctga tgcccctgat tgatgaaaat acagccctgg tgatggtaca gtatccagat
     1261 ttcttcgggc gcatctacga ctatacagcc ctggcagagg caacccacaa cgccggagcc
     1321 ttgttcgcag tggcggtaaa cccactttct ttggggttgc tgaaaccccc tggcgagttt
     1381 ggtgctgaca tcgttgtggg tgaaggtcaa ccattgggca tcccgctttc ttatggtgga
     1441 ccgtatctag ggatattcgc aactcgcaag gaatatgtgc gaaaaatggc ggggcgccta
     1501 gtaggagaga cggtagataa tcgcggacag cgcgcctacg tactcacttt gaccgctcga
     1561 gagcaacaca tccgccgcga gaaagccaca tccaatatct gcaccaacca ggggctgatc
     1621 gccctcgcct cagccatcta catggcgctg ctgggcaagc atggtttgcg ccaggtagct
     1681 gagctgtgct accacaaagc ccactacgct gcccgtaagc tgggtgcgat acgcggctat
     1741 cagctttgga atcaaacacc cttcttccac gagtttgtac tccgctgccc atcatccacc
     1801 gaggagatca acgctcacct cctggatcac ggaattctag gcggctatga tcttgggcaa
     1861 gcttatccta aattgaaaaa ccacttattg attgcggtta ccgagatgaa cagcaaagaa
     1921 gatattgatc tgcttgcaga agtcctgtcg gaggtgtccc atgggtgagc cattactttg
     1981 tgagatgtct gtacctgggc gagtcggttt tcgctaccca gcacctgacg ttcccatgga
     2041 gcccttgcca gaagcactgg tgcgtagcaa cctgcctcta ccagagctat acgaggtgga
     2101 tgttgttcgt cacttcacgc gtatctctca aatgaatcac agtatcgata ctggcttcta
     2161 cccccttggc tcctgcacga tgaaatacaa tcccaaggta aacgaagaaa cagcccggct
     2221 accaggtttt gcccacgtgc atccattgca accggttgag acggttcaag gaagcctggc
     2281 tttgatgtat gacttgcaga attggcttaa ggaaatcggg ggctttgcag gtgtgaccct
     2341 gcaacctgcc gctggcgccc agggtgagtt gaccggagtg ctaatacttc gcgcctacca
     2401 cgaaaagcgc ggacagacac agcgcaacaa aatactgatc cccgactcgg ctcacggcac
     2461 taacccggcc acctcagcca tgagtggtat gcgcgtggtc gagattaagt ccgatgcgcg
     2521 cggcaacgtc gacctggagg ctctgcgtct tgagtgcgat gacacgctgg ctggattaat
     2581 gctgaccaac cccaataccc tggggctctt cgacgagaac gtagaagaag tcatccgcat
     2641 ggttcacgaa gctggagggc ttgtttacgg cgatggcgcc aacatgaatg cgctgttggg
     2701 tatcgtacgc cccggagacc tgggcttcga tgtgctgcac tataatctgc acaagacctt
     2761 ctccacacca cacggcggcg gtggtcccgg ctccggtccg gtaggtgtgg ctgcccacct
     2821 ggtagatttt ctgcctggcc ctgttgtggg cattatcgaa gaggagaatg aagatgagga
     2881 catgcctccc ctctacggat taatcacccc accccatagc atagggcgtg tcaaatcctt
     2941 ccatggtcac tttggtatga tggtgcgcgc ctatacctat atgctcatgc acggaaaaga
     3001 gggcctgcgc ctaatcgccg actatgctgt acttaacgcc aattatttgc tggctcgctt
     3061 acgtgacaca tataaactgc catatgaccg ggtttgcatg cacgagttcg tcctggaagg
     3121 tcgctggccc gatgcacccg atatccacgc gttggatatt gcaaagcgcc taatggactt
     3181 caggttccac ccgccaacca actacttccc gctcatcgtt cacgaggcat tgatgatcga
     3241 accaaccgag actgagagca agcaaacgct ggatgcattt gcggacgcct tgatcaagat
     3301 cgcagaagag gcgcattcgg agccagatct gctcaagacc gccccgcaca tcacgccagt
     3361 cggacgcgtg gatgaggtca aagccgctaa agagctcgtc ctttgttgta cggtagatat
     3421 aagctagaac tgatctagtc aaggcgtatt gtctcacccc aaatggggta atgttgaata
     3481 ctaaccttca acacctcgaa tcttttattc ggggtgtttt aggtttgtat tgtttatttt
     3541 ataattccat ccttacgaat ttgacaatcc gtaactaggg cccttgcctg tgggatgatg
     3601 gcttcatgat aaaatacgtt tccctctcat caaattcaga aaatgagtgg tagagatttt
     3661 tcttttcatt aattggtata cgaacaacta ttatgagtaa atctgaatct ttgaaagtcc
     3721 agtttattgc tccaagtccc acagttgaag tacacctgga aaacggaaaa acaatctgtg
     3781 ggccacgcgg gacaccgatc ggtgagttcc taaaattatt ggaaggcgaa agcccggctc
     3841 ccattgtggg ggctgtggtt aacagcgagc tgcgagagct gacctatccg atccatgtgg
     3901 aatctcacgt ccgtccagtg accatggggg aagctgacgg tgcaagaatt tatcgccgct
     3961 caatagtttt tctactagaa tccgcatttg actattgctt ccctggagca gtgttaacca
     4021 tagaccactc cgtagcatct ggtggctatt attgccaggt ttctgaacgc cccgctctca
     4081 ccgaagagga gcttgccggt atggaagcac gcatgcgtca acttgttaag gcagacttag
     4141 ctttccaacg caaagaagtc ccattgaaag aggcgataga ttactttgcg ttaaaagggg
     4201 caggagataa agtacaactg ctcaaacacc gtgagaaaga ttacctgaca ctgtaccaac
     4261 ttcaaaattt ttttgattac catcatggct atatggtgcc atccacagga tatctaaaat
     4321 ggttttcttt gatcccaacc ggagatggtt ttacactgcg cttccctcgc cgccatgcgc
     4381 caacagtgat actgcccctc ccagattacc ctaagttgct ggccaccttt cgccagtatg
     4441 gcgactggct gcagcgactc ggtatctcca gcgtgggcgc actaaatgac gccatccagg
     4501 caggtcggag ccgtgaggta atcctggtct ccgaagccct tcacgaacag caaattgccg
     4561 agattgctgc ccatattgcc caacgacggg accagttgcg cgtggttctc gttgccgggc
     4621 cttcatcatc gggaaaaact actttttcca ggcgactctc cgtgcaactc ctggcacgcg
     4681 gaatctctcc tttcccgttg gagatggaca attattttgt ggaccgagat cagaccccac
     4741 ttgacgagaa tggcgagctg gattttgagt cgattggcgc gctaaatcac gaacgcctct
     4801 ctgaagactt gctccgcctt tccaatggtg aattggtaca aatcccggat ttcaatttta
     4861 agaccggcaa aagcacccct ggtgaagaaa ttcagctcaa atccaacgaa atcatcatct
     4921 tggagggcat tcatggtctt aatcccgctt tgatttccgg catcccctcg gagaatgctt
     4981 accgcatcta tgtctccgcc ctcacgcaat tgaatctaga tcgtcacaat cgtgtctcca
     5041 caaccgacac tcggttgatt cgtcgaatag tgcgcgatgc ccgcgagcgc ggctataccg
     5101 ctcaacagac catacaacgc tgggactctg tgcggcgtgg agaaaagcgg tatatcttcc
     5161 cttaccagga aaatgctgac gtgatgttca actcagcttt ggtgtatgag gtatccgtat
     5221 taaaacctct cgtcgagcca ctattgcggc aggtccctta cggaaaaccg gaatacatcg
     5281 aagccaaacg cctgttagcc tttgtccaat ggttcctgcc cctggaaagt gatttaatcc
     5341 ccgataactc gatcatgaga gagtttatcg gagaatctat cttgcgagat ttcactttat
     5401 ggcacaacca ctcggaaacc aagtaaatcg agacaagctg atgcgcacgg tgcgcgtgcg
     5461 caatgaccag caactgggca ctctcggtcg cctgcttacg acagtagctg aagagcgagg
     5521 cgatattggg gagatacgcc ttgttcaaga gggacatcgg gcgaatatcc gtgatatcac
     5581 gattttcgtt gatgatgaag cccaactgga gcgtgtgctg aaagccatgg ctggcaatcc
     5641 cggcacgcgc atcctggctg ttcgcgatga agtgctggaa ctacaccaaa aaggcaaaat
     5701 tgcaattcgc agccgatacc agattgactc tttatacaca ctgaggcgag tctatacacc
     5761 cggcgttgcc aatgtatgcc taaaaatcgc cgctgagcca tctctggcgc gacagtacac
     5821 ggcgatcagc catctcgtag ccatcgtcac agatggtacc gccattttag ggctgggaga
     5881 tatcggcccc ttggcgggga tgcccgtgat ggaaggcaaa gccatgctca tggaaactct
     5941 ggtggggcta tcgggtgttc ccatcctgct caacacaaag gatgtggata caatcgtaaa
     6001 aacggtggta agcattgctc ccacattcgc cgccatccaa ttggaggaca tctccgcccc
     6061 gcgctgtttc gagattgaaa gacgcctgca agagcagcta gatatcccag tgatgcatga
     6121 tgaccagcat ggcaccgcgg tagtcactac agccgcccta tttaatgcct gccgcaaaac
     6181 aggcatgaat ctgaaggaag cagtgatcgg ccagatcgga ttgggtgctg caggaaatgc
     6241 catcggtcgc atgctgatgg cgctcactgg caattcaatc ctcggcgctg acatttcaga
     6301 ggatgccctg caacggtttg aacaaatagg tggcctgcgc tcaaaccttt ctgaggtgat
     6361 ggatcgagca aatatcatca tcgctaccac cggagtacct ggtttgatta aacctagcca
     6421 ggtaaggcaa gggcaaatca tactggctct ttccaatcca aaccctgaaa tagatccaga
     6481 tgcagctatc gccgccgggg ctgccttcgc tgccgatgga aaatccgtca acaacgtact
     6541 tggcttccca ggcattctca ggggcgcgat cgacacctat gcaccgcgaa tcacagatga
     6601 aatgtacctg gctgccgccc aaacaattgc agagctcacg ccccgcgaag aattggttcc
     6661 aaacccactc gataaacagg tgcatcaagc ggtagcccga gcagtagctc agaaagcgat
     6721 agagcagggg ctggcgcgcg ccgaatttgt gccatatctc gaagaatagc gtgcactcat
     6781 cccgtgtcct gggcatattc gccgtttttc ttgtagctct gtttttagct ttagttatcc
     6841 tgaccggttt cagtcaggtc tctgccacgg agcaaactgc caccggacaa gcccatctct
     6901 ttctagcccc aacaattgca ccaaacgatg gtccctgggt ggtggtggct tatttctcca
     6961 accgagaaca ggtaaatcga gtcgcagaac ggattgagcc ctgggaagtg aattacgacc
     7021 agggctttat gttgctcgag gtggataaag accaatacca gtggctttta gaactaggac
     7081 ttcaagttgt gatcgcccag gagcttactg aactgctcca ccgcccgcaa tttgccctcc
     7141 cagaacaaga agcaggcatc cctggttatc cttgttaccg aactgttgaa gagacctacg
     7201 ctaccggcca acagatcgcc attgatcacc ccggcctggc aacttggata gatatcgggg
     7261 atagctggaa aaagttcgcg ttcggtagtg attctggtca cgatatcatg gtgcttcgct
     7321 taaccaatgc ccaggtttca ggctctaagc cgaagctatt cattctctca tctatccatg
     7381 cccgtgaata cgccccagct gagttgaata cccgattcgc tgagtacctt gttaacaact
     7441 acggcacaga ccccgatgca acctggcttc tggatcacca cgagatacac ctgctattaa
     7501 acggtaaccc agatggtcgc gagcgcgccg aggcgggaaa atactggcgc aaaaataccg
     7561 ataacaacta ctgctcggat acagactcgc gtggcgccga tctaaaccgc aattttgagt
     7621 ttttatgggg ttgttgcaac ggctctagcg accgccaatg tgacataact taccggggac
     7681 cagctccagc ttctgaaccg gagacgcagg ccattcagaa ctacgtccgc tcgatctatc
     7741 ccgaccagag ggaggacgcc ctgtcctcgc ctgccccagc cgatgctagc ggcgttttca
     7801 tcgatctgca cagctacggc gagttagtct tgtggccatg gggcttcaca tatgatgctc
     7861 ccccgaatca tgaagctctt cgcacccttg gacggaagtt cgcttatttc aatggataca
     7921 cgccagagca atccatcgaa ctctacccca ctgacggcac aactgacgat ttcgcctatg
     7981 gtgagctcgg aattgcagga tataccttcg agttgggcac cgcttttttc cagagttgct
     8041 ctaccttcga aggggatatc ctctcagata atctagccgc actactctat gcagccaaaa
     8101 tcgtccgcac accctaccaa acacccgccg gaccagatag catggctgtc aatatctcgc
     8161 caaagttcgc agtgaaagga caagaagtaa ttttaagcgc cacgatcgat gatacccttt
     8221 attcaggcgg tgaattaaca caatctatcg tggcggcgga atattatatt gatgtgcccc
     8281 cctgggtatc atctacaacg cccgtctcgt ttgcgatgtc cgcccaggat ggcggctttg
     8341 acgagcccag cgaaccagtc atggcttcgg ttgatacaac aggcttagcg tcaggacgcc
     8401 atattttgtt cgtccgcggc ttggatgccg atggcaattg gggcgcattt actgctacat
     8461 tcttatggat tgaagaggat caagtttatc tccccattat tgtaaaataa gtgcgaattt
     8521 ctttcgagta taattgacct atgcataaac ccgccaacct tgaccccata gattatttgg
     8581 taatcggcca cctgacttgt gatctgaccc cagatggtta tcgcctggga ggcaccgcgg
     8641 cttactcaac ccttactgct catgctttag gtcttcgggt aggtgttgtc acctcctggg
     8701 gagaggaaat tccgttgggg ccgctgcgcc acatcccaat cgccaatttt ccaaccgata
     8761 aaagcaccat ctttgaaaat atccacacac ccgaagggcg tgtgcaagta atccacaatg
     8821 tagcccccaa tttgggtttc aatcttgtac ccgacccgtg gcgcaatccc gccatagtcc
     8881 atctgggccc cttagtccag gaggttgagc cgtctctcat ccgtaatttc ccgtccgcat
     8941 tgatcggtgt cactccgcaa ggcttcctgc gtggctggga tgccaaaggg cacgttcact
     9001 tcagcgaatg gcctgaatcg acctttgtct tgcagagagc gggcgctgca gtaatcagcg
     9061 tggaagacgt ggaaggcgat gaagatcgca tcgctgaaat ggcctcctcc tgccgcattt
     9121 tagctgttac cgagagagaa caaggggcgc gcctgtactg gaacggcgac gtgcgtcgct
     9181 ttcgcccccc ccaaattcaa tttgtggatg atactggagc aggtgatatc tttgcggctg
     9241 ccttttttgt gcggctctac acaacccgtg atccatggga ggcggctcga tttgctacgt
     9301 atctggctag cttctccgta actcgcagcg ggttggatgc gatccccacc caggaagaaa
     9361 ttcaggaatg catggttgag gtattataga tggcgcgcac gtacacgctg gtcaaccaaa
     9421 agggaggagt tggcaagaca accacagcga taaacctggg cgcttacctt gcatattttg
     9481 gtcagcgtgt tctcctggtg gatcttgacc cgcaagcgaa tgcgacttct tgtttgcgga
     9541 tcgataagca taacgtgcgg ggaggcacct acgaggcgtt gattggctca tctccagcgt
     9601 ccggtcatat tttgcacaat ccgcgtctga aactctctat tctgccttct acaccagaac
     9661 tggccggcgc agaggttgag ttagtacccg aactggcgcg cgaaacccgg ctaaaaaaag
     9721 ctctcgatcc cataattgat cgctacgact atgttctcat tgattgtccc ccatcgttag
     9781 gcctattgac tttgaatggc attgtctctg cccagaatgg ggtcatcatt ccagtgcagt
     9841 gcgaatacct ggctcttgaa gggctgagcc agctcaccca gacactaggc cgtgtgcgca
     9901 atgcactatt ccctcaattg agcattcgcg gtgtgctgct gaccatgttt gatggtcgca
     9961 cgaatctcgc caacgatgta gtcaaagagg tgcgccgctt cttccccgac caggtgttcg
    10021 agacagttat ccctcgcagc gtacgtctgg cggaagcacc ctcctatggg caaccgattt
    10081 cctactatgc accgtcttct ccaggtgcgg tcgcttacgc agccttggca cgtgaagtct
    10141 tggagagaga tggggtgcat atccccattc ctgaggagat ttgacgacca tgccgcgccg
    10201 acccggactt gggcggggtc tcgaagccct gatccctgaa agcgaacaac ctacttcaac
    10261 tgggacaagc tatatccctc aaaaccgcat cgtcccaaac ccgcgccagc cacgttcaaa
    10321 gatcgaccca caggagttgg ccgaactatc tgcctctatt aaagaacatg gagttctgca
    10381 accattgctc gtcagttatg acaatttatc cggcaattac acactgattg ccggtgagcg
    10441 ccgattgctg gccgcgcgcc aggcaggctt agaaaccgta ccagctatcg ttcgcgaggt
    10501 aaatgaacaa caacgcctgg aactggctct gattgagaac ctccagcgtg ccgacctgac
    10561 cccccttgag gctgcagagg cctaccggca acttgttgag gaatattctc tctctcatga
    10621 aaatattgcc gaccgggttg gcaagagccg agccgcggtt accaatacct tgcgcctgct
    10681 caaattgccc attcgcgtac aacaagcgct tgctgacggt cgtatcagcg aaggacatgc
    10741 ccgcgctttg ttagccctcc ccacacccga agctcaagaa gcggcgctgc gcacgatact
    10801 caaacaggat ttaaatgtgc gacagaccga agaaatcgtg cgcaagctca gtggactccg
    10861 tcctgtgtcc acacccaaag caaatccatc tccagagctg actgccttag aggaaagact
    10921 gcggacgaag ctcggcacac gggtaaagct caatcgctcg cgtaaagggg gaaccctggt
    10981 catccacttc tattctgatg aggagctaga cgctttactc gaaacactgc tgcaagaatg
    11041 accagagttc ttatctgcgc aaatatctat acgctcaacc cgcaacagcc ccatgcaacc
    11101 gccatcgtca ttgatggcga acgaattttg aggctgggtg cgcggcagga gatattaacc
    11161 gaattcgccg gaaatggaca tcgtgttgag atcctggatg cacgccatca gacgctcatc
    11221 cccggcctga tcgatgcgca ttttcaccta gaacaatatg ctctggcaca gcaaatggtg
    11281 gactgcgaaa ctccaacgcg tgctgagtgc gagcgtaggg tcgctgagcg agctcagcag
    11341 atgccaccgg gagaatgggt gttagggcat ggctggaacc aaaatgattg gcctgagggc
    11401 ttcggcaaca acaccgacct cgacgcagta
//
LOCUS       DBNGSX010000452        12931 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_528586, whole genome shotgun sequence.
ACCESSION   DBNGSX010000452 DBNGSX010000000
VERSION     DBNGSX010000452.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12931)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12931)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12931
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_528586"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..927)
                     /locus_tag="AC16CB_13445"
     CDS             complement(127..927)
                     /locus_tag="AC16CB_13445"
                     /inference="COORDINATES: protein motif:HMM:NF020296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HIR3343519.1"
                     /translation="MPAINLARLRKQTAVLVDHFNQPEAFLRLLHDLLDHYADHILRH
                     GQAGEPPPLIQAYRVPAPMLRQLAQELAPRASEDAPLTLALCDALWTQPYLEPRILAA
                     TLIGQAPLTSVDDVLSRVEKWAESTSEERLIEALANRGLMRVQQEQPERLLLQIEAWL
                     GTESLMLRRIGLRALISLISDASFENLPVALKLVTPYVRVAPLPLKPDILDVLAALAR
                     RSAKETAYFLRQNLDVSGDTDTAWLIRQSLRFFPVETQDSLRDAIRRT"
     gene            complement(935..1459)
                     /locus_tag="AC16CB_13450"
     CDS             complement(935..1459)
                     /locus_tag="AC16CB_13450"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR3343520.1"
                     /translation="MNITVFCSSQTKPGEPAYQEGLRLGQLIGSAGHTVLTGGYIGSM
                     EAVSRGAAEAGGWVVGVTCDEIEAFRPGKANKWVHEERRFSTIRQRLYALIDGCDAAI
                     ALPGGIGTLTEVAMMWSHLQTGVISPRRLVLIGPGWRDSMHTFWRVFDPHVPERFRTL
                     LSFAPDADAAMSTI"
     gene            complement(1456..1764)
                     /locus_tag="AC16CB_13455"
     CDS             complement(1456..1764)
                     /locus_tag="AC16CB_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016561985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HIR3343521.1"
                     /translation="MELRDLIQRMHEFVSAKGWYETDSPRPQTLRNLAISLNLEASEV
                     LEHFQWGEQVVNNDELAKELADVALYLLQLASLAGIDLEQAILNKLEQNYQRKWDQPR
                     "
     gene            1790..4426
                     /locus_tag="AC16CB_13460"
     CDS             1790..4426
                     /locus_tag="AC16CB_13460"
                     /inference="COORDINATES: protein motif:HMM:NF013871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3343522.1"
                     /translation="MRIILTHEQADFDAIASLLGAYLNDETSVPVLPRRMNRNVRAFL
                     TLYGADLPFVDPRDLPGEPIDFVTLVDTQSLVSVRGMSETTRVHLIDHHPLRDDLPQD
                     WVITVSGTGANTTPFVEMLHEHEHVLTTIQATLLLLGIYEDTGSLTYDRTTPRDLRSA
                     SYLLEQGASLQIAANFLNHPLSKAQQVLYDHLRQTAEYHHIHGYTIIVACGDAREMGE
                     ELSTVAHKLRDLLDPDALCMLVATRGGVQFIARSTNDNIDVAEVAAYFGGGGHERAAA
                     SLIKDRTVEQVHDELVSILPDYVNPAITVAQIMSPDPQLLAPDTPVEEAATRMQRYGY
                     EGYPVVRDGKLIGLLTRRAVDRAISHKLKVNAASLMEAGEFTVQPDDSIEHLQRLMTD
                     TGWGQIPVIDPQTGEIIGIVTRTDLIKTLSPRPRIPGRQNLAERLERALPPARLALLK
                     SVAKVAHEQKVALYIVGGFVRDLLLERPSLDFDLVVEGDAIALARALSRKHGGRVTSH
                     ARFGTAKWLLTDAGKNQGDSRKATNRYDKLSNPNQEFLDLITARTEFYTYPSALPTVE
                     RSSIKLDLHRRDFTINTLALRLDGRYYGELYDYWGGFADLRQGIVRVLHSLSFVDDPT
                     RMLRAVRFEQRFGFRIEQRTLELLIEAIPLLERVSGDRIRHELDHILIEERAVEMLAR
                     LSELKLLVAIHPRLQWDEWFAKRLSAALTAVPESDWDTLSELDIWSLRMDLAYILWVL
                     RFPASVVREITARLKFSAVLSRTILAAHQVWKDLPGLAGATPRTVVERLDGVPLLALY
                     AVHLASDQEDLRQLILNYVVRWKEVKPTITGHDLRARGLPPSPHYKLILKTLRDAWLD
                     GSVKTEEQEAKLLEELIGNRKD"
     gene            4505..5023
                     /locus_tag="AC16CB_13465"
     CDS             4505..5023
                     /locus_tag="AC16CB_13465"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3343523.1"
                     /translation="MIDYLARLIIRQATQSDLPALEWNGEYTHFRLLYRDIFQSSQHG
                     EAILWVAELPETGIIGQLFVQLISSRKELADGFQRAYIYGFRMKSEYRGYGLGTYMLK
                     YVEIDLVNRGFQIAVLNVARENEDARLLYERQGYQVVAAEPGQWSYLDENGNRRWVNE
                     PAWRMEKILDSN"
     gene            5199..7142
                     /gene="nusA"
                     /locus_tag="AC16CB_13470"
     CDS             5199..7142
                     /gene="nusA"
                     /locus_tag="AC16CB_13470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR3343524.1"
                     /translation="MKNEFVLAFNEVLEEKQLPRDVILEALQAAMVSAYRRAVNASNA
                     QQIEATVDLETGKVSIFAEKEVVDVVQDERTEVALEDARRVDPEAEFGGMVIVDSTPD
                     NFGRVAAQTARQVIQQRIREAERDAQMQFFGKQLGEIVSGVVQSVNSQAITIGLDMKA
                     EGIMPRNQQIPGERFRVHDRVRALLMEVKPTSRGPQIILSRAHRNYLRRLLENEVPEI
                     YQGLVEIRSIAREPGQRSKVAVSALQPGVDPVGACVGMRGVRIQAIVRELNDEKIDVI
                     EWSSDPTIYIAKALSPARVNGVYLNELAKGAKTATVVVPEDQLSLAIGRDGQNARLAA
                     KLTSWRIDIKSLPEAATDALAKLQRDPEYAFMLEQEGEVFPQVELILAKKSEGRPVTP
                     EEYTVLNQFVDRVEHGLLLKREKELKAEQERLRVAEANVPSAAYDIPLEDLGISLRFS
                     TILAGAGYTTVGDLMLQMSLDSNEVLRLDGIGVKAMEEIETALANLTFPEPEAEIEPS
                     AEIPVEEAQPELVEAVSEIEPEPEAEIDQEAVSEEELAPVAAEAEQEAVPEPVVEGQT
                     EEIPEEEPTSFDELFKLRADVMELVDEVDEEDEEEGADRPAQKKKKKKKKRYVELEYD
                     PDKDVVLARKKRKRGVGEWGEDW"
     gene            7149..7484
                     /locus_tag="AC16CB_13475"
     CDS             7149..7484
                     /locus_tag="AC16CB_13475"
                     /inference="COORDINATES: protein motif:HMM:NF016204.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HIR3343525.1"
                     /translation="MARRAGRRKHIPQRTCVGCRDVMSKRSLIRIVRGPHGVQVDPTG
                     KMPGRGAYLHDRRSCWERGLKGSLAHALKATLTVEEREQLSSFAATLKSLDDVTEGSF
                     SPEQQIDGS"
     gene            7641..9437
                     /gene="infB"
                     /locus_tag="AC16CB_13480"
     CDS             7641..9437
                     /gene="infB"
                     /locus_tag="AC16CB_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR3343526.1"
                     /translation="MSENGHKTIELPSNITVRDLAQVIDASPIEIIKALMANGVMANI
                     NQLIDFDTAAVVAAEMGYEASLETPELSKTEEVGEIPLWRRIIADEDPSKLVVRPPVV
                     TILGHVDHGKTTLLDAIRHTNVVEGEAGGITQHIGAYQAEHNGRIITFLDTPGHAAFT
                     AMRARGAQGADVVVLVVAADDGVMPQTREAVAHAKAAKVPIVVALNKIDKPNADADQV
                     KQQLAEIGLVPDEWDGDTLVVPISAKKRLGLDDLLEAIVLVADNLDIRANPKGEVIGT
                     VIEASIDRSKGVMATLLVQNGTLGVGDVVIAGNAYGRLKAMFDYRGRQVRKAGPSTPA
                     SVMGLSDVPVAGDLFQRVESDREARGIVAERLQAKQQAVVSQEAAVTLEQLFDRVQAG
                     EVRELRLIVKADVQGSLEPIISSINDLSTKDIAINILYAETGNIGENDVMLAAASKAI
                     VIGFNVQADSAARRLAESEGISIRLYDIIYRLTEDIEKALKGMLAPVEVETVIGHAEV
                     RAVFRISKIGQVAGCRVLDGELRRNALMRVLRNGETVHKGSLASLKHLQDDVREVRTG
                     FECGVALKGFDSFEEGDILECFVLEKTPVVQL"
     gene            9467..9841
                     /gene="rbfA"
                     /locus_tag="AC16CB_13485"
     CDS             9467..9841
                     /gene="rbfA"
                     /locus_tag="AC16CB_13485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR3343527.1"
                     /translation="MRSERIADRVREELSEMMIYEISDPRLNGVSITDVTVDRELAFA
                     DIYFSSLEGTSRSQEIKEGFDHAQGYLRRELARRIDLRSFPRLRFHYDPTFERAENIE
                     KLFASLNQPSKEQGGEQDTQDG"
     gene            9834..10799
                     /locus_tag="AC16CB_13490"
     CDS             9834..10799
                     /locus_tag="AC16CB_13490"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013528.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR3343528.1"
                     /translation="MAESIHEQIRRKIQASRRILVATHMRPDGDAIGSLLGLGLALQD
                     TGKIVQMISTDGVPLSYRHLPGSQEVIQQPDGEVDMVIILDCSDIHRAGKSLNGFGAP
                     DLNVDHHITNLNFGRLNLVDTKAVATAEIVAENLSNWGLSLTPAVAAALLTGLITDTL
                     GFRTFNMTPKALRLAADLMEAGAELPELYMRALVQRSFEAMRFWGSGLSRLEREGQIV
                     WATLSLADRQTAGYSGRDDADLINALSAISDAVISIIFVEQPNGKVKVSWRSQPGIDV
                     SAIALKFGGGGHVSASGAELIGDLDRVREDVIRETSKILEEKVIV"
     gene            10894..11808
                     /gene="truB"
                     /locus_tag="AC16CB_13495"
     CDS             10894..11808
                     /gene="truB"
                     /locus_tag="AC16CB_13495"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR3343529.1"
                     /translation="MMNKDHVKNVVSGVLVVDKPIGLTSHDVVQIIRRGTGIRRAGHT
                     GTLDPRASGVLVVLVGPAVRLSEYVSASDKRYQATIRLGTATDTYDADGTFTTPTISV
                     DDITEDLFTEILEKFIGTIEQVPPPYSAVKVKGRKAYEMARQGEEVDLEPRTIQVYSL
                     EVLEWAPPEVVIDVYCSSGTYVRSLAHDLGRELGCGAYLIGLRRTKSGRFTLRDAVPM
                     RRLQEAFTAGDWYRHLIPAAEALADWPMVELDADQVELVRHGHRVAADPGSKGWARGV
                     SQQGDLVALLEIDPETLEWQPKKVFFQS"
     gene            11810..12745
                     /locus_tag="AC16CB_13500"
     CDS             11810..12745
                     /locus_tag="AC16CB_13500"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HIR3343530.1"
                     /translation="MRHYRLLDGVSLKDVWLTIGTFDGVHLGHQEIIGRVVDGARQSG
                     VPAVAVTFYPPPTVVLKKRSDPSYLTFPEQRAQLLGELGIDVVITHPFDMQVAAMSAR
                     EFVASLHAHLHMKNLCIGYDFALGHDREGNQAVLHQLGDEFGYSLQVVPPVQVDGKVV
                     SSSLIRAHLAEGEVEKAARLLGRPYQLSGEVVLGDGRGKSLGIPTANLAVLAESALPK
                     AGVYVCQTQVNGRRWGAVTNIGVRPTFENQPTQPRVEAHILDFGHEIYGQEIELEFLA
                     HIRDEARFPNPQALIQQIHQDISRARSYLATQLNL"
BASE COUNT         3177 a         2986 c         3664 g         3104 t
ORIGIN      
        1 ctttatgtta ttttttgagc aggtctccag actggcagcc atttatattt gccgctagtc
       61 ggtctagtca gagcgggtgg attataccat taatgttgat cgtgtggctt tgtatttgtc
      121 tgacgattag gttcgccgta tggcatcccg taggctatct tgggtttcga caggaaaaaa
      181 tctcagcgat tggcggatca gccaagcagt gtctgtatct cctgatacat caaggttttg
      241 acgtaaaaag taagccgttt ccttcgctga tcgcctggcg agcgcagcca gtacatccaa
      301 gatatccggc ttcagcggaa gtggtgccac acgcacgtag ggagtgacca gttttaaggc
      361 aactgggaga ttttcaaagc tagcatcaga aatcaatgag atgagggctc tcaaaccgat
      421 gcgccgtaac atcaggctct ctgtgcccag ccaagcttcg atctgcagca gcaagcgttc
      481 gggttgttcc tgttgcaccc gcatcaaccc tcgattggct aaggcctcga ttaggcgctc
      541 ttcggaggtg gattcagccc atttctccac ccgtgagagc acgtcatcaa cagacgttaa
      601 tggtgcttgc ccaattaaag tcgcagctag aatgcgcggc tccaggtagg gttgagtcca
      661 aagtgcatcg cagagtgcca gggttagcgg tgcatcttcg gaggcacgcg gcgctagttc
      721 ctgggctaat tgccgtaaca ttggggctgg aacccggtaa gcttggatga gtggtggggg
      781 ctcacctgcc tgtccatgac gcaggatgtg gtcggcgtaa tgatccaaca ggtcgtgcaa
      841 gaggcgtaaa aaagcctcgg gttggttgaa atgatctacc aggacggcgg tttgttttcg
      901 cagccgggct aagtttatgg caggcataga ataactatat cgtgctcata gctgcatcgg
      961 cgtctggggc aaaggagagc agcgttcgga atctttccgg tacatgggga tcgaaaactc
     1021 gccaaaatgt gtgcatggaa tcgcgccagc caggcccgat aagcaccaga cgacgcgggg
     1081 agattacccc ggtttgcagg tgcgaccaca tcatggctac ctcggtcagg gtgcctatac
     1141 caccaggcag cgcaatggct gcatcgcagc catcgatgag ggcataaagg cgctgccgta
     1201 tggtggaaaa gcgacgctct tcgtgtaccc acttgttggc tttgccagga cggaaggctt
     1261 cgatctcatc acaggtgacg ccgaccaccc agcccccagc ctccgctgcc ccgcgcgaga
     1321 cggcttccat tgagccgatg taccccccgg tgagcaccgt gtggcctgct gagccgatca
     1381 gttgtcccag gcgcaatcct tcttggtaag ctggctctcc cggtttggtt tgtgagctgc
     1441 aaaaaacggt gatgttcatc tcggctgatc ccatttccgc tggtagtttt gttccagctt
     1501 gtttagtatg gcctgctcca gatcgatgcc agccaggctg gcgagttgta gcaggtaaag
     1561 ggctacgtcg gcgagctcct tagccagctc atcattattc accacttgct caccccactg
     1621 gaaatgctct agaacctcgg aggcttccag attgagagag atggccaggt tgcgcagggt
     1681 ttgtggccgc gggctgtctg tttcatacca cccttttgca gacacaaatt cgtgcattcg
     1741 ctggataagg tcgcgaagtt ccataggtgt tgattataat acatacagta tgcgtatcat
     1801 cctgacacac gagcaagctg attttgacgc tatcgcctca ttactggggg cgtacctgaa
     1861 tgacgagacc tctgtcccgg ttctgccacg ccgaatgaac cgcaatgtcc gcgcctttct
     1921 gaccctatat ggcgcagact tgccttttgt cgacccccgc gatctgccgg gtgagccgat
     1981 cgatttcgtg accctggttg acacgcaatc cctggtcagc gtgcggggga tgagcgaaac
     2041 cacccgcgtg cacctgattg accaccatcc attgcgggac gacctgcctc aggattgggt
     2101 gatcactgtt tcgggtacgg gagcgaacac taccccattt gtcgagatgc tgcatgagca
     2161 tgaacacgtg ctgaccacaa tccaggctac cttactcctg ctagggatct atgaggacac
     2221 tggatcgcta acttacgacc ggactacgcc acgtgatctg cgctccgcaa gctatttgct
     2281 ggaacaaggt gccagcctgc agattgctgc taattttctc aaccatcccc tgtccaaagc
     2341 gcagcaggtg ttgtatgacc atctgcgcca gaccgcggag tatcaccaca tccacggata
     2401 tacgatcatt gtggcgtgtg gagatgcccg tgagatgggg gaggagctat ctacagttgc
     2461 tcataaattg cgcgatttgc tggaccccga tgccttgtgc atgctggttg ccacgcgggg
     2521 cggggtgcag ttcatcgccc gctctaccaa tgacaatatc gatgtcgccg aagtggcagc
     2581 ctactttgga ggcggaggtc atgagcgggc ggctgccagc ttaatcaaag accgcactgt
     2641 tgaacaagtc cacgacgaat tggtgagtat cctgccagat tatgtcaacc cggcaattac
     2701 ggtcgcccaa attatgtctc ccgacccgca attgctcgct ccagacactc cagtagaaga
     2761 ggctgccacc cggatgcagc gctatggtta tgaaggctat cctgtggtgc gcgatgggaa
     2821 attgattgga ttgctgacgc gtcgggcagt tgatcgcgcc atttcccata aactcaaggt
     2881 aaacgcagcg agcttgatgg aagctggcga gttcaccgtt cagcctgatg attcaattga
     2941 gcatttgcag cgcttgatga ccgacactgg ctgggggcaa atcccggtga tagatccaca
     3001 gactggagaa atcattggaa ttgtcacccg taccgacctg atcaagaccc tctcgccacg
     3061 cccacgaatt cctgggcgcc aaaacttagc tgagcgcttg gaaagggctc tgcccccagc
     3121 gcgtctcgcc ttgctaaagt cggtcgctaa ggtagctcat gagcagaaag tcgccttgta
     3181 tattgttgga gggttcgtcc gcgatctgtt attagaacgc cccagcctgg attttgacct
     3241 ggtggtcgaa ggtgatgcga tagccttggc gcgcgcttta tcccgcaagc atggcgggcg
     3301 cgtcacaagc cacgcgcgct ttgggacggc aaagtggtta ttaacggatg caggtaaaaa
     3361 tcaaggagat tcccgtaaag ctacaaatag gtatgacaaa ttaagcaatc caaatcaaga
     3421 atttcttgat ctgatcactg cgcgcacaga gttctatacc tatcccagcg ctttgcccac
     3481 cgtagagcgc agcagcatta agcttgatct tcaccgacgt gattttacga ttaacacctt
     3541 ggcgttgcga ctggacgggc gctactatgg cgaattatat gattattggg gcggatttgc
     3601 tgacctgcgc caagggatcg tgcgtgtctt gcactcccta tcctttgttg atgatcccac
     3661 ccgcatgcta cgggctgtgc gttttgagca acgctttgga ttccgtattg agcagcgcac
     3721 cttggagtta ttgatcgaag ccattccgct gctggaaaga gtctcgggtg accgcatccg
     3781 gcatgaatta gaccacatct tgatcgagga gcgtgctgtc gagatgctgg ctcgcctgag
     3841 cgaattgaaa ttactggtcg cgattcatcc ccgattgcaa tgggatgagt ggttcgccaa
     3901 acgattgagt gccgcgctta cggcagtccc agaatcggat tgggatacgt tatctgaatt
     3961 ggatatctgg tcactgcgta tggatttagc atacatctta tgggtgctcc gctttccagc
     4021 ctcagtagtc agagaaatca cagcccgttt gaagttctcc gcagtgcttt ctcgcacaat
     4081 attagcggct catcaggtat ggaaagactt gccgggttta gccggcgcga ccccaagaac
     4141 tgttgttgaa cgattggatg gtgtgcctct tctagcactc tacgctgtcc atttggcaag
     4201 tgaccaggag gatttacgcc aattaattct caattatgtt gtgcgctgga aagaggtcaa
     4261 gccaaccata actggccacg atttacgggc acgcggtttg ccacctagtc cgcattacaa
     4321 gctaatactc aaaactctgc gtgatgcatg gctagatggg agcgtgaaaa ctgaagagca
     4381 agaggcaaaa ttgctggaag agctgatcgg taatagaaaa gattagctcg ttggggtatt
     4441 ttagacaatg gaagaggcgg gcaaataccc cctcgctttg tttctaaggt agttccttat
     4501 cgatatgata gattatctcg ccaggcttat catccgccaa gctactcaat ctgacctgcc
     4561 tgccctggaa tggaatggag aatatactca ctttcgcctg ctataccggg atatcttcca
     4621 gagttcccag cacggggaag ccatattgtg ggtggctgaa ttgccagaaa caggcattat
     4681 tggtcagctt ttcgtccagc ttatcagttc tcgcaaagag cttgccgatg gatttcagcg
     4741 cgcatatatc tatggatttc gaatgaagtc cgaatatcgt ggctatgggc tgggtaccta
     4801 tatgcttaaa tatgtagaga ttgatctggt gaatcgtggc ttccaaatcg ctgtgctcaa
     4861 tgtggctcga gaaaatgagg atgctcgtct actatatgag cgtcaggggt atcaggttgt
     4921 tgcagccgag ccagggcaat ggtcttatct cgacgagaat ggaaaccggc gctgggtaaa
     4981 tgagcctgcc tggcggatgg aaaaaatact agattcgaat taaaatccta taagaagggt
     5041 tgacttaacc ttgtctgcct cacgaattcg tgctaaaata ttgccagggc aggcaagcga
     5101 cgttgatatg aactggtgaa aagtgggcaa cccccacttt tctgcatata ataaagagtt
     5161 gctgcctaaa ggatcaagca caggagataa ggtaagttat gaagaacgag tttgtcctgg
     5221 ctttcaacga agtgttggaa gaaaaacagc ttcccagaga tgttatcctg gaagcgctac
     5281 aggctgcgat ggtatcggct tatcggcggg cggttaacgc ttccaacgca cagcaaattg
     5341 aagccaccgt ggatttggaa actggcaagg tctctatttt cgctgaaaaa gaggtcgtag
     5401 atgttgtcca agatgagcgc accgaggttg cattggaaga cgcgcgtcgg gtagaccccg
     5461 aggcggaatt cggtgggatg gtgatcgtcg attcgacccc agacaatttt ggaagggtag
     5521 ctgcgcagac ggctcgccag gtgatccaac aacgcatacg tgaggctgaa cgtgatgccc
     5581 agatgcagtt ttttggcaaa cagctcggag agatcgtcag cggagtagtg cagtcagtca
     5641 attcccaggc catcacgatt ggtttggata tgaaggccga agggattatg ccgcgcaacc
     5701 agcaaatacc tggagagcgg ttccgggtgc atgaccgggt gagggcattg ctgatggagg
     5761 ttaaacccac ctcgcgcggc cctcagatca tcctttcccg cgctcatcgc aattatttac
     5821 gccgtctttt ggagaacgaa gtccctgaaa tctatcaagg actagtcgaa attcgctcca
     5881 ttgctcgcga gccagggcaa cgatcaaaag ttgctgtatc ggctttgcag ccaggtgttg
     5941 atccggttgg ggcctgtgtt ggcatgcgag gtgtgcgtat tcaggccatc gtccgggaat
     6001 tgaatgatga gaagatcgat gttatcgagt ggagttcaga tcctacaatt tatatcgcca
     6061 aggcgctcag cccagcgcgc gtaaatgggg tatatctgaa tgaacttgcc aaaggggcaa
     6121 agacagctac agttgtagtc cctgaggacc agcttagttt agcaatcggg cgggatggtc
     6181 agaatgctcg gctagcagcc aagctgactt cctggcgtat cgatattaag agcctgccgg
     6241 aggcggccac ggatgcattg gctaaactgc aacgcgatcc agaatacgca ttcatgttgg
     6301 agcaagaagg ggaagtgttc cctcaggttg aattgatcct tgcgaagaag tctgaaggac
     6361 gcccagttac tcctgaagag tacacagtgc tcaaccagtt tgttgatcgg gtagaacacg
     6421 gcctgctact caagcgagag aaggaactga aggctgagca agagcgactg cgcgtagctg
     6481 aagccaatgt gcccagtgct gcatatgata taccgctcga agacttgggt atttcattgc
     6541 gcttttctac aatcctggct ggtgcggggt atactaccgt cggtgatttg atgcttcaga
     6601 tgtcattaga ctcgaacgag gtgttacgac tggatggcat tggggtaaaa gctatggaag
     6661 agatagagac agctctggct aatctcacct tccccgagcc tgaagctgag atagaaccat
     6721 cggcggaaat accagttgaa gaggctcaac cagaattagt agaagctgta tccgagattg
     6781 aacccgaacc agaagctgag attgaccaag aagcggtctc ggaagaagaa ttagcaccgg
     6841 ttgctgctga agctgaacag gaagcggttc ctgaacctgt tgttgaaggg cagacggaag
     6901 aaatccctga agaagagccg acctctttcg acgaactgtt caagttacgc gctgacgtaa
     6961 tggaattagt tgacgaagtg gatgaggaag atgaagagga aggtgcggat cgacccgctc
     7021 agaagaaaaa gaagaagaaa aagaaaagat atgttgagtt ggagtatgat ccagataagg
     7081 atgttgtgct cgctcgcaag aaacgcaaac gcggtgtggg cgagtgggga gaggattggt
     7141 gatcctctgt ggcaaggaga gcaggcaggc gtaaacatat ccctcaacgc acctgcgtag
     7201 gttgccgaga tgtcatgtca aagcggtcgt tgatacgcat tgtacgcggc cctcatgggg
     7261 ttcaggttga cccgactggc aaaatgcctg gaagaggagc gtatttacac gaccgacgct
     7321 catgctggga aagaggctta aaagggtctc tggctcatgc gctgaaggca acattgacag
     7381 tggaagaacg agaacagctg tcatcctttg ctgcaacgtt aaaatcattg gatgatgtta
     7441 cggaaggctc tttttctccg gaacagcaga ttgatggtag ctgacctaga aagttgcaat
     7501 tttgggcgaa gaatgcaatc gccactgatg ggctatgtga tgtaaatcaa tagatcacat
     7561 gaccctaatc ggttatgcct gagacgctca gatgggcgct gaatggcgat agcggcttcg
     7621 ctgtaatttg gaggtgcacg atgagtgaaa acgggcataa gacgatagaa ctaccgtcga
     7681 acattacagt tcgagattta gcccaagtga tcgatgctag tcccattgaa atcatcaaag
     7741 ctctgatggc aaatggggtc atggcgaata tcaatcaatt gattgatttc gacaccgcgg
     7801 ctgtcgtagc ggctgaaatg ggctatgagg cgagtcttga aacgccagaa ttgtctaaaa
     7861 ctgaggaagt gggtgagatc ccgctttggc gaagaattat tgctgatgaa gatccaagca
     7921 aactagtagt tcgcccgcca gttgttacca ttctgggcca tgtggatcat ggtaaaacga
     7981 ccttgctcga tgctatccgg catacaaacg ttgttgaggg ggaagcaggc ggcattaccc
     8041 agcatattgg tgcataccaa gcggagcaca acgggcgaat aattacattt ttagacaccc
     8101 ctggacacgc agcctttacc gccatgcgtg ctcgcggtgc ccaaggagca gatgtggttg
     8161 ttctggtagt ggctgcagac gatggagtga tgccccagac acgcgaagct gtggcacatg
     8221 ccaaggcagc caaggtgccg attgttgtcg cactcaataa gattgacaaa cccaacgccg
     8281 atgctgacca ggtaaaacag cagctggctg agataggctt ggtccccgac gaatgggatg
     8341 gcgacaccct ggtagtgccg atttcagcaa agaaaagact tggactcgat gaccttttag
     8401 aggctatcgt gcttgtagca gataacctgg atattcgcgc taatccgaaa ggggaagtga
     8461 tcggaaccgt gattgaggct tctattgacc gttcgaaagg cgtgatggct acattgcttg
     8521 tgcagaacgg gacactggga gtgggtgatg tggtaatcgc tggaaacgca tatggaagat
     8581 tgaaagcgat gttcgattac cgtggtcgtc aagtgcgtaa agctgggccg tcaactccgg
     8641 cttctgtcat ggggttgagt gatgtcccgg ttgcaggcga cttatttcaa cgcgttgaat
     8701 cagatagaga agctcgtggc atagttgcag aacgattgca ggccaagcag caggctgtag
     8761 tgtctcagga ggccgcggta acactggagc agctattcga ccgcgtgcaa gcgggggagg
     8821 tgcgcgagtt acgtctgatc gtcaaagcgg atgtccaagg atcattagaa ccgattatct
     8881 cttcgattaa tgacctcagc accaaggata tcgcaattaa tatcctttac gcggaaacag
     8941 gcaacatcgg tgagaacgat gtcatgctgg cggcggcatc caaggcgatt gtcattggct
     9001 tcaacgtgca agcagattct gccgcgcggc gtctcgcaga atcggagggc atttccatcc
     9061 ggctttatga catcatatat cgtctaactg aagacattga aaaggcattg aaaggcatgc
     9121 tggcgccagt agaggtggaa acggttattg gtcacgcaga ggtgcgagct gtttttcgca
     9181 tctcgaagat tgggcaagtg gcgggttgtc gtgtattaga cggcgaactc cggcgaaacg
     9241 cgctcatgcg tgttctgcgc aatggcgaga cggtgcataa aggatcgctg gcatctctaa
     9301 agcatctgca agatgatgtc cgtgaagtgc gcaccggatt tgaatgcggc gttgcgctta
     9361 aaggctttga cagtttcgaa gagggcgaca tccttgaatg ctttgtgcta gagaaaacgc
     9421 ctgtggtgca gttgtaacga tcgagaggta aattgtggta tcgaagatgc gctctgagcg
     9481 tattgcagac cgagtacgag aggaactctc ggagatgatg atctacgaga tctcggatcc
     9541 acgtctgaat ggcgtatcaa ttacggacgt gaccgtagat cgcgagctgg cattcgctga
     9601 tatatatttt tcttctctgg aagggacatc ccggtcacag gaaatcaagg aaggatttga
     9661 ccatgcgcag ggatacttgc ggcgtgaatt agcgcggcgt attgacctaa ggagttttcc
     9721 gcggctgcgg ttccattatg acccgacttt tgagcgtgcc gaaaacattg aaaaattatt
     9781 tgcctctttg aatcaaccat cgaaggaaca aggaggcgaa caagatacac aagatggctg
     9841 aaagtattca tgaacaaatt cgaagaaaaa ttcaggcatc gcgacggatt ttagtggcaa
     9901 cccacatgcg cccagatggc gatgcaattg gatcgctctt gggtttgggc ttggcattac
     9961 aagataccgg gaaaattgtt caaatgattt ccaccgatgg agttccactt agttaccgcc
    10021 atctccctgg cagtcaagaa gtaattcaac agccggatgg ggaagtggat atggtgatca
    10081 ttttggattg ttctgatatt catagagcag gtaaaagcct gaatggcttt ggtgcacctg
    10141 acttgaatgt tgaccaccat ataacgaatt tgaatttcgg tagattgaac ctggtcgata
    10201 cgaaggcagt cgcaactgcg gagatcgttg cagaaaatct atccaactgg ggactctcat
    10261 tgacaccagc cgtcgcagcg gctctattaa cgggccttat cacggatact cttggtttcc
    10321 gcactttcaa tatgacgccg aaagcattgc gcttggcggc agatctaatg gaagccgggg
    10381 cggaattgcc tgagttatac atgcgcgctc tggtgcagcg atctttcgag gctatgcgat
    10441 tttggggatc tgggctttca aggctggaga gggaaggaca aattgtctgg gctacgttat
    10501 ctttagcaga tcgccagact gctgggtatt caggtcgtga tgatgctgac ctaatcaacg
    10561 ctctttcagc cataagtgat gctgtgatat caattatatt tgttgagcaa cccaatggta
    10621 aggtgaaggt tagctggcgg tctcaacctg gtattgatgt ttcagccatc gcattgaagt
    10681 ttggtggcgg cgggcatgta agcgcgtctg gtgcagaatt aataggggac ctagataggg
    10741 tgcgcgagga cgttatccgt gagacaagca agattctcga agaaaaggtt atcgtttaat
    10801 ggtaaagtgt cgtcctgcct gccaaatctt aatccattca tgggaggagg gagatatgcc
    10861 aacctctaaa gactctggaa taggggaaag acaatgatga acaaagatca tgttaaaaat
    10921 gttgtatcgg gtgtattggt ggtggacaag cccattgggc tcacatctca tgatgtagtg
    10981 cagatcatcc gcagggggac tggtatccgg cgcgcgggac atacagggac gttagaccca
    11041 cgtgcatctg gtgtgcttgt ggtcttggtt ggccccgcgg ttcgactcag cgagtatgtt
    11101 tcagcatctg acaagcgcta ccaggcgacg attcggttgg gtactgcaac ggatacctat
    11161 gacgcagatg ggacattcac cacacccaca atatcggtgg atgacattac ggaggatctc
    11221 tttaccgaaa ttttggagaa attcatcggc accattgaac aggttccgcc tccttattct
    11281 gccgtaaaag tcaaaggacg caaggcgtac gagatggcgc gccaaggaga agaggtagac
    11341 cttgaaccgc gtacaatcca ggtatacagt ctggaagtgt tggaatgggc gccacctgag
    11401 gtcgttatcg acgtatattg ctcgtcgggg acttatgtgc gctcactagc acatgacctg
    11461 ggtcgtgagc tgggttgtgg tgcttatttg atcgggttgc ggcggacaaa aagcggacgt
    11521 ttcaccctac gggatgcagt ccctatgcga cgtctgcagg aagcattcac tgctggcgat
    11581 tggtatcgtc acctgatccc cgctgcagaa gcactggctg attggcctat ggtcgagttg
    11641 gatgccgacc aggtcgaact ggtccggcat ggtcatcgcg tagcggcaga tcctggcagc
    11701 aaaggttggg cacgcggggt tagccagcag ggcgatttgg tggcattact tgaaattgat
    11761 ccagaaacat tggaatggca gcctaagaag gttttcttcc agtcatgata tgcgacatta
    11821 tcgtttattg gatggcgtct ccttaaaaga tgtctggcta acgattggta cgttcgatgg
    11881 tgtccatctc ggccaccaag agatcatcgg cagggttgtt gatggagccc ggcaatccgg
    11941 agtcccggcg gtggcggtta ctttttatcc cccgccaact gtggtgctga aaaagcgctc
    12001 cgatccctcc tatctaacat ttcctgaaca gcgcgcccag cttctaggag aactgggtat
    12061 cgatgtggtt atcacccatc catttgatat gcaggtggct gccatgtcgg cgcgcgaatt
    12121 tgtagcttct ttgcatgcgc acctccatat gaagaatctg tgtattggct atgattttgc
    12181 ccttgggcat gatcgggagg gtaaccaggc tgtcttgcat caattgggag atgagtttgg
    12241 ttactcgctc caggtggttc caccagtgca ggttgatggc aaagtggtgt cttccagttt
    12301 gatccgcgcc catctggctg aaggagaagt ggagaaggca gcacgtttgt taggacgacc
    12361 gtaccagcta agcggtgaag tggtgcttgg agatgggcgc ggcaaatcgc tcggtatccc
    12421 aacggctaac ttagctgttc tggctgagag tgcattacct aaggcgggtg tctatgtttg
    12481 ccagacgcag gtcaacggtc gaagatgggg ggcggttacg aatatcggtg tgcgccccac
    12541 ctttgaaaac caacccaccc aaccgcgtgt agaggcgcat atactggatt ttgggcatga
    12601 aatctatggg caggagattg agcttgaatt cctggcacac attagggatg aagcccgttt
    12661 cccaaatccg caagctctga tccagcaaat tcatcaggac ataagccggg cgcggagtta
    12721 tttggcaacc caattaaatt tatagatatt agctacttaa ttgatggcgt agattcaagt
    12781 agtaagtgca actttgcagg ctgaaaaaga ggtaagctga ggtaggataa gagcttcttg
    12841 atcagccaaa tcaaaggagc gcttatgaga acctacaaac agcttaccca ggaacaaaga
    12901 taccagattt atgcattgat gaaaatgggt c
//
LOCUS       DBNGSX010000453         4869 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1291024, whole genome shotgun sequence.
ACCESSION   DBNGSX010000453 DBNGSX010000000
VERSION     DBNGSX010000453.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1291024"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..321)
                     /locus_tag="AC16CB_13505"
     CDS             complement(109..321)
                     /locus_tag="AC16CB_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343531.1"
                     /translation="MWRSINKFLDKAAEFLAHRKGLLPVLGIILVLANSVLRFMPNGG
                     WLAETDFMLHLGVILGFIGILLAWAL"
     gene            complement(330..1658)
                     /gene="hflX"
                     /locus_tag="AC16CB_13510"
     CDS             complement(330..1658)
                     /gene="hflX"
                     /locus_tag="AC16CB_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIR3343532.1"
                     /translation="MAKKIPEPTQRPRERAFMVGVEIRNQDNLLVLQDSLAELELLAG
                     TAGLNVVGQATQRLDHPNPQTFIGPGKVEEVHALVEEVRADVVLFDEELSPRHLRELE
                     KIFGENARIVDRTALILDICAQHAATREGALQVELAQYEYRLPRLTRAWTHLARQAGG
                     GGGRSGSVGGVGLRGPGETQLEVDRRDIRRRIAHLKVELEKVRTHRQQYRLRRKRSRI
                     PVVALVGYTNAGKSTLLNRLSEADVYVADQLFATLDPTTRRVELPSGNMILVTDTVGF
                     IQKLPTELIAAFRATLEEIVEADLLMHVVDVTHANAKAQAEAVYQTLSEIEADHIPTL
                     SVLNKIDLLADPENARLALANFPNAVAISALTGEGIQDLLGRVNEHLYETYSPVIVRL
                     PYQEGSLIAAFHDQGQVERVEQTHQGVLIQGRLPGRLLARYQPFLVQRAG"
     gene            complement(1660..2889)
                     /locus_tag="AC16CB_13515"
     CDS             complement(1660..2889)
                     /locus_tag="AC16CB_13515"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3343533.1"
                     /translation="MLQKIKPAWISGVSQRLAKGEGTRLLFRVQLDNFYQLLIQAVDS
                     GNPIVMNGLLDEWIQSRTQTDVNLQKSTLIPILEQFILVFYEIATENLDQAVGLQTLG
                     AILPVLTHAIGYSTQVEARLYLDQYLKQEEESRMSLERLERSKSDFISIAAHELKTPL
                     TLIEGYSAMLREIFRDIKDNDQSQILIKGIENGTHRLRQIVDDMIDVSMIDNQLLELK
                     YQPIRIGRLLDLIQREFHDVAATRNIAIEKNSFPGSEETTFGDEERIYQALHNVVSNA
                     LKFTPDGGSVTIDGRLLPGFLEIIVIDTGIGIDPINHARIFEKFGRLGEASRHSSGKT
                     KFMGGGPGLGLPITKGILEAHGGTIWVESEAHDDVGCPGSKFHILLPIRKRPPDEKMA
                     KLFRSSIQTGELKDPYK"
     gene            complement(2954..3868)
                     /locus_tag="AC16CB_13520"
     CDS             complement(2954..3868)
                     /locus_tag="AC16CB_13520"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR3343534.1"
                     /translation="MTKNNRSITLLALTTIASLTLLISLGLLAINSINSPNAHFDGER
                     AMGDVERQVSFGPRTPGSVAHAQTVEYIRDELENHNWNVRIQETSLLDKPIRNIIGQR
                     GEGRPWMILGAHYDSRLLADRDADPSKHNQPVPGANDGASGVAVLLELGRVIPQDLPG
                     QVWLVFFDAEDNGRIAGWDWILGSQAFVNALTDHPDAAIIVDMIGDSDLNIYQERNSD
                     VALTQEIWAIAQSLEYSNEFISQYKHSMLDDHTPFLRAGIPAVDIIDFDYPYWHTTED
                     TPDKVSATSLQIVGDTLLKFLTQRNRKQ"
     gene            complement(3897..>4869)
                     /gene="trmFO"
                     /locus_tag="AC16CB_13525"
     CDS             complement(3897..>4869)
                     /gene="trmFO"
                     /locus_tag="AC16CB_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009924616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HIR3343535.1"
                     /translation="PVIIATGPLTSPRFSDAIRRLTGSEHLFFYDAIAPIISKDSIDM
                     DIAYRASRYERGEQKVGDYINCPLTRDEYYDFVEALTNAERIELKVFESELEKGVRAG
                     AERFFEGCLPVEILGQRGADTLAYGPLRPVGLPDPRTGKRPYAVVQLRQDNLAASLYN
                     MVGFQTNLKFSEQKRVFRMIPGLEKVDIVRYGQMHRNTFIFSPAYLKPTMQFNRRNDL
                     FFGGQITGVEGYVGNIATGLLAGWNAARLLQGKETLVLPRTSMLGALCHYVTHASPED
                     FQPMKANFGILPELEGTNSKIRGKRMRARAYTERAMRDLSEFLESFN"
BASE COUNT         1233 a         1255 c         1086 g         1295 t
ORIGIN      
        1 gcagcccgct ttatcgataa tttcaagaaa tatgtagaga aaatcccagg agaagtcctt
       61 gccgctggtc caaagatctg atccgatcgc tatcgataag cgagaagttt acaaagccca
      121 tgccagcaag ataccaataa accccaggat cacccccaag tggagcatga aatcggtctc
      181 agccagccag ccgccattgg gcatgaaacg caacacgctg ttcgcaagta ccaagatgat
      241 ccctaatacc ggcaaaagcc ctttgcggtg agccagaaat tccgcagctt tatcgagaaa
      301 tttattaatg ctacgccaca tacgctaaat tatcctgcgc gctgcacgag gaacggctgg
      361 tagcgtgcca gcagccggcc tggcaagcgt ccctggatta atacgccctg gtgggtttgc
      421 tcaacgcgct ccacctgccc ttgatcgtga aaagcggcaa tcaaactacc ctcttgataa
      481 ggcaggcgca ctatcacagg agagtaggtt tcataaaggt gttcattaac gcgacccaat
      541 aggtcttgaa ttccttcccc ggtcaaagcc gagatagcca ccgcatttgg aaaattggca
      601 agtgccaggc gggcgttctc tgggtcagcc aatagatcga tcttgttcag cacgctcaaa
      661 gttggtatat gatctgcctc aatctcactc aaagtttggt aaacggcttc tgcctgtgcc
      721 ttggcgttgg cgtgggttac gtcaaccaca tgcattaata aatccgcttc gacgatttct
      781 tctaatgtgg cgcgaaacgc cgcaatgagc tcggtgggca gcttttgtat aaatccaacc
      841 gtatcagtca ctagaatcat atttccgctc ggaagctcca ctctacgggt tgtcgggtca
      901 agtgtggcaa agagttggtc cgccacatag acatcggctt cagacaaacg attgagcagc
      961 gtggattttc cggcattggt gtagccaacc agcgccacga cggggatacg cgagcgctta
     1021 cgccgtaagc ggtattgctg tctatgggtc cgcaccttct ccaattctac tttgagatga
     1081 gcgatacgcc tgcgaatatc ccgccggtca acttcaagct gggtctcgcc aggaccgcgc
     1141 aaccctacgc cgcccacgct cccagatcgg ccaccaccac cacccgcttg gcgcgcaagg
     1201 tgcgtccagg ctctcgtcaa cctgggaagc cggtattcat actgcgccag ttctacctgt
     1261 agcgcaccct ctctggtggc tgcatgctgg gcacagatat ccaatatgag ggcagtacgg
     1321 tcaacgatgc gggcattttc gccgaagatt ttctcaagct cacggagatg gcgcggcgag
     1381 agttcttcgt cgaatagtac aacatccgct cgcacctctt cgaccagagc atgtacttct
     1441 tccacctttc ctggcccgat gaaagtttga ggattgggat gatccaagcg ttgagttgcc
     1501 tgcccaacga catttaatcc agctgttccc gctagaagtt caagctccgc aagagaatct
     1561 tgcaatacta gcagattgtc ttgatttcgg atttcaacac ccaccataaa ggcacgttcg
     1621 cggggtcgct gagtaggttc aggaattttc ttcgccatat tacttataag gatccttcaa
     1681 ttcgccggtc tgaattgatg accgaaatag cttggccatt ttttcatctg gaggtcgttt
     1741 acggattggg agcagtatgt ggaatttgga acctgggcat cccacgtcat catgtgcttc
     1801 tgattcgacc catattgttc cgccatgagc ttcaaggata cctttagtta tcggcaatcc
     1861 taatccaggt ccaccaccca taaacttcgt ttttccgctg gagtggcgcg atgcctcgcc
     1921 caagcgacca aatttttcaa aaattcgcgc atggtttatt ggatcgatgc caatacctgt
     1981 atcgatgaca ataatctcca aaaaccctgg tagaagccga ccgtctatag taaccgagcc
     2041 tccgtccggc gtaaatttta atgcgttgct gaccacatta tgcaatgcct gatagatgcg
     2101 ttcttcatcg ccaaatgttg tctcctcgct gccaggaaac gagttctttt caatggcaat
     2161 attcctggtc gcagccacat catgaaactc tctctggatt aaatctaaaa gacgaccaat
     2221 tcggattggc tggtatttta attcaagcaa ctggttatct atcatcgaca catcgatcat
     2281 gtcgtcgaca atttgcctca atcggtgggt gccgttctct atccctttaa tcaatatctg
     2341 actctggtca ttatccttta tgtcccgaaa gatttccctt agcatagccg aatatccctc
     2401 aattagggtt aggggggttt ttaattcatg tgctgcaatg gaaataaaat cggatttgct
     2461 ccgctccaaa cgctctaaag acatccgaga ttcctcttct tgttttaggt attgatccag
     2521 atagagacgg gcttcaactt gggttgaata tccgattgca tgagttagca ccggtaaaat
     2581 cgcacccaat gtttgaagcc ccactgcttg atctaaattt tctgttgcaa tctcataaaa
     2641 tactagaata aattgctcta agataggaat taatgtgctt ttttgaagat ttacatcagt
     2701 ctgggtcctt gattgaatcc attcatcgag caacccattc atcacaatag gattgcctga
     2761 atcgacagct tgaataagca gctgatagaa attgtcaagc tgtacgcgaa acaacagccg
     2821 ggtgccctcg cctttagcta gccgctgtga cacacctgag atccaggctg gcttaatctt
     2881 ctgaagcatc tctcttgcag gcatacgtgt agtttacgac atgaaacact ttttgccaag
     2941 aatgcggggg tgactattgt ttacgatttc gttgggtcag aaatttcaag agcgtatcac
     3001 ccactatttg taggctagtt gctgatactt tatcgggagt atcttctgtg gtatgccagt
     3061 aaggataatc aaaatcgatg atgtccactg caggaatgcc ggcacgtaaa aatggggtgt
     3121 gatcgtcaag catagagtgt ttgtattgtg aaatgaattc gtttgaatat tccaatgatt
     3181 gtgcaattgc ccaaatttct tgagtcaatg ctacatctga atttctctct tgatagatat
     3241 tcagatcaga atccccgatc atgtccacaa taatggctgc gtctggatga tcagtcagtg
     3301 cattgacaaa tgcttgcgag cccaatatcc agtcccaacc tgctattcgg ccattatctt
     3361 cagcgtcaaa aaaaacaagc cagacctggc caggcagatc ttgtggtata acgcggccca
     3421 actccaatag tacagccact cctgacgctc catcattggc tccagggacg ggttgattgt
     3481 gtttggaggg gtctgcatcc cgatctgcta gcaaacgcga gtcataatga gccccaagga
     3541 tcatccatgg tcgcccttct ccacgctgcc caataatatt tcggatgggc ttatccagta
     3601 aagatgtctc ctgtatccgc acattccaat tatggttttc gagctcgtca cgaatatact
     3661 caacggtctg tgcatgtgca acgctaccag gcgtgcgcgg cccgaaagaa acctgccgtt
     3721 ccacatcccc catcgctcgc tcaccgtcaa aatgggcatt tggagagttt atactgttga
     3781 ttgcaagcaa tcccaggctg atgagtaaag taaggctggc tattgtagtt agggcaagta
     3841 aggtgatgga tctattattt tttgtcatgt aatggctatg cgaaatgttt tatttgtcaa
     3901 ttgaacgact caagaaattc actaagatct ctcatggctc gctcagtata agctctcgct
     3961 ctcatgcgct tcccccttat cttcgagttc gtcccttcaa gttccggtaa gatgccaaaa
     4021 tttgctttca tcggctgaaa atcctctggg ctagcatggg ttacatagtg gcacaatgcc
     4081 cccagcatgc ttgttcgcgg tagaaccagc gtttctttgc cttgcaatag tcgcgcggca
     4141 ttccatccgg ctagtaaacc cgtagcaata ttgcccacat atccttccac acctgtgatt
     4201 tgaccgccaa aaaacaaatc atttcgacga ttgaactgca ttgtcggctt caagtacgct
     4261 ggagaaaaga taaatgtatt gcgatgcatt tgcccgtatc gcacaatatc gaccttctcg
     4321 agccctggga tcatccgaaa aaccctcttc tgctccgaaa attttaaatt agtttgaaac
     4381 cctaccatgt tgtacagaga ggcagccaga ttatcctgac gtaattgaac aaccgcgtat
     4441 gggcgtttcc ccgttctagg atccggtaaa cccacagggc gcaaggggcc atatgcaagg
     4501 gtatccgctc cgcgttgtcc cagtatttcg accggcagac agccctcaaa aaaccgctcc
     4561 gctccagcgc gaacgccttt ttctagctcg ctttcaaata ctttcagctc tatccgctcc
     4621 gcattagtca aggcttcgac aaaatcgtag tattcatccc tggtcaaagg gcaattaata
     4681 taatccccca ctttttgttc acccctctcg taccgcgatg cgcggtatgc aatatccata
     4741 tcgatagaat ctttcgagat gatcggagct atcgcatcat aaaaaaataa gtgctcgctg
     4801 cctgttaatc tccgaatcgc atccgaaaat ctaggcgacg tcagtggccc agtagcaata
     4861 attacaggc
//
LOCUS       DBNGSX010000454         3530 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1567697, whole genome shotgun sequence.
ACCESSION   DBNGSX010000454 DBNGSX010000000
VERSION     DBNGSX010000454.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1567697"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..415
                     /gene="rpsO"
                     /locus_tag="AC16CB_13530"
     CDS             143..415
                     /gene="rpsO"
                     /locus_tag="AC16CB_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR3343536.1"
                     /translation="MSIDKETKTKVIEEFSRHNTDTGSPEVQVAILTSRINQLTDHLR
                     AHKHDESSRRGLLKLVGQRRRLLAYIRRNDFPRYTALTDRLNLRRK"
     gene            677..2905
                     /locus_tag="AC16CB_13535"
     CDS             677..2905
                     /locus_tag="AC16CB_13535"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR3343537.1"
                     /translation="MKPDVKQFQATLGSKVFTFETGKLAGQAGGSVTLRLGDTVVLAA
                     ATMSADVRPGVDFVPLTVDYEERMYAGGRIPGSFFRREGRPTEEAILTARLTDRPLRP
                     LFPKNLRNDIQVILYSLSTDGENPIDILAVNAASAAVLLAGLPWAGPVGAVRVGWIEG
                     DFILNPTFADLEYSDLDLRMAGTRDAILMVECGASAVSEEIMVEALEFGHKAMQPLID
                     AQERIVAEAGVTKREYISAPANEDLAVTVQERAGAQLNHILQQPYIKSERNEAIDALK
                     TSLLEDLAGEDESQAPLVKEAFDQLLKSVVRQTILEHNQRPDGRGLNDIRDIWCEVDV
                     SPRAHGSGLFTRGETQVLTLATLGTPREAQELDSLSPSESKRYIHHYNFPPFSTGEVR
                     PLRGASRRDIGHGALAERALVPVIPLETDFPYTLRLVSEVLSSNGSSSMASVCGSTLA
                     LMDTGVPIKAPVAGVAMGLIKEGERYAILTDILGAEDHYGDMDFKVAGTQAGITALQM
                     DIKIKGINTTIMRQALEQARQARLYILNKMMEVIPAPNPTLKPHAPRITVVQIPVEKI
                     GAVIGPGGKTIRSIQEETGAKIDIGEDGSVFIATADGEAARRAVERVEAITETPVIGR
                     IYTGRVVRTTEFGAFVEILPGTDGMVHISQLDTERVNKVEDIVRVGDELTVMITDIDP
                     QGKIRLSRQAVLEGWTPEEAKMRDSRRTGGGGRPSYGGGRPGGRGGDRGDRGRSGGSN
                     RR"
     gene            2929..>3530
                     /locus_tag="AC16CB_13540"
     CDS             2929..>3530
                     /locus_tag="AC16CB_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HIR3343538.1"
                     /translation="MNADIRIDALLPPEMAKRAEYLGVRKAEAKISAMFSLAVLAGIF
                     IALGAMFATTVSAGTSGVLPFGVTRLLVGLVFCLGLILVVVGGAELFTGNNLIVMAWA
                     GGKVSTSAMLRNWVVVYLGNFVGSIITAILVIFSKQYAFGGGAVGQSMLNIAIGKIHF
                     GFFQALALGVLCNMLVCLAVWMTFSARSTIDKIMAVIFPIS"
BASE COUNT          795 a          993 c          990 g          752 t
ORIGIN      
        1 atcgtcagac aaatacaaag ccacacgatc aacattaatg gtataatcca cccgctctga
       61 ctagaccgac tagcggcaaa tataaatggc tgccagtctg gagacctgct caaaaaataa
      121 cataaaggag aaagcagacg tcatgtcgat tgataaagaa actaaaacga aggtcatcga
      181 ggagttttcc cgccacaata ctgataccgg ctcacctgaa gttcaggttg ctatactgac
      241 ttcccgcatc aatcagctca ccgatcacct gcgtgcccac aagcacgatg aatcatctcg
      301 ccggggattg ctcaagctgg ttggccagcg tcgcaggttg ctggcctata tccgccgcaa
      361 cgactttcca cgctacacag cactgacgga tagactgaac ctgcgcagaa aataaacata
      421 tcacaaggag atggcgtgca agctcagcca tcttttatta tggttctaac gggatgcatg
      481 ggaatgatcc ccagaattcg gtgtatagcg ggtatacctg ctatacaccg aattctgggg
      541 cttctctcgc tcaacccaaa agattccaaa tttttttcat gcccaccggt taggtattga
      601 aagatggcga taaccctgtg caggtgttgt cggcagctct cagtccctga ccaggtgggg
      661 taattcagga gataaaatga aacccgatgt taagcaattc caagccacgc ttggaagcaa
      721 agtatttacg tttgagaccg gaaagcttgc cggacaggca ggcggctcgg tcaccttgcg
      781 cctgggcgat actgtggtcc tcgcagcagc caccatgagt gctgatgttc gccctggtgt
      841 cgacttcgta cccctcacag tggattacga agagcgcatg tatgccggtg gacgtatacc
      901 cggctcattc ttccgccggg aaggcaggcc taccgaggag gccatcctga ccgcccgcct
      961 taccgaccgc ccattgcgcc ccctattccc caaaaacctg cgcaatgaca tccaagtcat
     1021 cctgtactca ctctccaccg atggcgagaa tcctatcgac atcctggctg tcaacgcggc
     1081 ttcggctgcg gtgctgctgg ctggtctccc ctgggctggt cctgtcggcg cagtgcgcgt
     1141 aggctggatt gagggagact tcatcttgaa cccaaccttc gctgacctgg aatactccga
     1201 tctggacttg cgcatggctg gcacgcgcga tgcgattctc atggtggaat gtggtgcctc
     1261 ggctgtatcc gaagagatca tggttgaagc cctggagttc ggacacaaag ccatgcagcc
     1321 gctgattgat gcccaggaac gcatcgttgc agaagccggc gttaccaaga gagaatacat
     1381 ctccgccccg gccaacgagg atctggcagt gaccgtgcaa gagcgcgctg gagcgcaact
     1441 gaaccatatc ctgcaacaac cctacattaa atccgaacgc aatgaagcca tcgatgcgct
     1501 caaaacctcc ctgctagagg atctggctgg ggaggatgag tcacaggcgc ctcttgtaaa
     1561 agaagccttt gaccagctgc tcaagtcggt cgtgcgccag accatcctgg agcacaacca
     1621 gcgtcctgat ggccgtggtc tgaacgacat ccgcgatatc tggtgtgagg tggacgtcag
     1681 cccgcgggcg catgggtctg gattattcac acgtggcgag acgcaggtgc tcaccctggc
     1741 taccctgggc acacctaggg aagctcaaga actcgacagt ctgtcgccgt ctgagtctaa
     1801 acgctacatt catcattaca acttcccgcc tttctctact ggcgaggtca gacctttgcg
     1861 cggggcatcc cgccgtgata ttggtcatgg cgcgctggca gaacgagccc tcgtccctgt
     1921 gataccgctt gaaacagatt tcccttacac tttgcgcctg gtgtctgaag tgctatcttc
     1981 aaacggctct tcgtctatgg catcggtgtg tggctcgacc ctggcgctca tggacactgg
     2041 cgtgcccatc aaggcgccgg ttgcgggagt tgccatggga ctgatcaagg aaggggagcg
     2101 ctacgccatc cttaccgaca tcttgggcgc agaagaccat tatggagata tggatttcaa
     2161 ggtcgctggg acccaggcgg gcatcaccgc cctgcagatg gacatcaaga tcaaaggcat
     2221 taataccacc atcatgaggc aggctctcga gcaggcccgc caggcgcgcc tctacatttt
     2281 gaataaaatg atggaagtca tccccgcacc aaatccgacc ctgaaaccgc acgccccgcg
     2341 catcaccgtg gtgcagatcc cggtcgaaaa gatcggagcg gtcatcggac cgggtggcaa
     2401 aaccatccgg tccatccagg aagagacagg cgccaaaatc gacatcggcg aagatggctc
     2461 ggtgtttatc gccacagccg atggcgaggc ggcacgacga gcagtggagc gtgttgaagc
     2521 catcaccgaa accccggtta tcgggcgcat ctacaccggg cgcgtggtgc gcaccaccga
     2581 gttcggcgcc ttcgttgaga tcctgccggg caccgacggc atggtgcaca tctcccagct
     2641 tgatacagag cgcgtcaata aagtggaaga catcgttcgc gtgggcgatg agctcaccgt
     2701 gatgatcacc gacatcgacc cgcagggcaa aatccgcctc tccaggcagg ctgtgctgga
     2761 aggctggacg cccgaggaag ccaaaatgcg cgatagccgg cgcactggcg ggggtggtcg
     2821 cccctcatat ggaggtggtc gtccgggtgg tagaggcggc gaccgtggcg atcgcgggcg
     2881 ctcagggggt tcaaacagaa gataattttt gcttaggaga gacgcaatat gaatgctgac
     2941 attcgaattg atgctcttct tcccccagaa atggccaaaa gggccgaata tctaggggtc
     3001 cgtaaggccg aggctaaaat ctcggccatg ttttccctgg ctgtgctggc tgggatattt
     3061 attgccctcg gagcaatgtt cgccaccacg gtttcggcag gcacatccgg cgtgttacct
     3121 tttggggtaa cacgcctttt ggttggtctg gtgttctgcc tggggctcat cctggtagtg
     3181 gtgggtggag ctgagctctt taccggcaac aacctgatcg tgatggcctg ggcaggtggc
     3241 aaggtgagca catctgccat gctgcgcaac tgggtggtcg tgtacctggg caacttcgtt
     3301 ggctcgatta tcacagccat cctggtcatc tttagcaagc aatatgcctt cggtggaggg
     3361 gcagtcggcc agagcatgct caacatcgcc attgggaaga ttcactttgg cttcttccag
     3421 gctttggcac tgggagtctt gtgcaacatg ctcgtctgcc tggcggtctg gatgaccttc
     3481 agcgcccgca gcacgatcga caagattatg gcggtgattt tccccatctc
//
LOCUS       DBNGSX010000455         2433 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1359299, whole genome shotgun sequence.
ACCESSION   DBNGSX010000455 DBNGSX010000000
VERSION     DBNGSX010000455.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1359299"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /locus_tag="AC16CB_13545"
     CDS             <1..1278
                     /locus_tag="AC16CB_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343539.1"
                     /translation="WLQAHLSLLQRQAARWQRESRAEYLLLREQDLQDAESWAAQHPQ
                     ELSQNDRDFLKVCQQLRSREEEGRASAENQLKLEAAEKVAEAERRRAEEQALAAKKLR
                     KRFLVATLLLIVALLSTGAAIFAGVQASESASEAQTAEVIANSERETAEKAKSTAVAA
                     EADALNQKGTAEYNAAIAATNEFKAKTANAIADSQREKAQTLSAQLAATVTQNIFDFE
                     TYKVTKENIQQTNSAKQVKTQEAYQAEITSLKSNIQINQTEIARLLNTIQGTPTLTTP
                     IAMSMSAPIPSRTPTPTPADSPYISFLNVKIRAVMCNEQIVKDNRCQARPGATIKVEL
                     EYIIVAEDYCPYCIDEIQIGFSHTDPLTCIYQGIPDRNGTQGKAEIEITMPKEAGIYN
                     LAVDRAQDYSCPSWWWNNRPPGEDQYFFRIEVP"
     gene            complement(1542..1940)
                     /locus_tag="AC16CB_13550"
     CDS             complement(1542..1940)
                     /locus_tag="AC16CB_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase domain-containing
                     protein"
                     /protein_id="HIR3343540.1"
                     /translation="MVNAQDPGQEWNMAHPAKMAQALDKGTAKQRLREIALLSVGTGG
                     FPKFLTTQDADWGWTQWARPLVDIMLEGSLEVAHYQCKSILGKRYHRIAARLNKPIGL
                     DSLESIPELEQVGQIYDLDEAVQFVKTYFV"
     gene            complement(1996..2388)
                     /locus_tag="AC16CB_13555"
     CDS             complement(1996..2388)
                     /locus_tag="AC16CB_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HIR3343541.1"
                     /translation="MLIHGRTIFAGNAQGEALVTSMGISFYGGVDPESGIVVERGHEL
                     EGLSIAGKVLAFPTGKGSTVGSYTLYRLKKNGLAPAAILNSECETITAVGCIIAEIPC
                     VDHISIEKIRTGEKLRVDAENAVIEVLA"
BASE COUNT          626 a          701 c          613 g          493 t
ORIGIN      
        1 tggctccagg cgcacctcag ccttttgcag cgccaggctg cccggtggca gagggagagt
       61 cgcgccgaat acctcttgct gcgcgagcag gacctgcaag atgccgagtc ctgggcggcg
      121 caacaccccc aggaactcag ccagaacgac cgggatttcc tgaaagtctg ccagcagctg
      181 cgcagccgcg aggaggaggg gcgcgcatcc gccgagaacc aactcaagct ggaggcggcc
      241 gaaaaggtag ccgaggcaga gcgacgccgc gctgaagaac aagccctggc ggccaagaaa
      301 ttacgcaagc gttttttggt agccacgctg ctcttaattg tcgccctgtt atccaccgga
      361 gcggccattt tcgctggggt tcaagcaagc gaaagcgctt cggaagccca aactgcagag
      421 gtgatcgcca actcggagcg ggagaccgcc gaaaaggcca aatcaaccgc agttgccgcc
      481 gaagcagacg ccctaaatca aaaagggacc gccgagtaca acgctgccat agccgcaacc
      541 aacgaattta aagctaagac tgctaatgcg attgccgact cgcagcggga gaaggctcaa
      601 acactcagcg cacaacttgc cgcgaccgtc acccaaaata tatttgactt tgaaacatac
      661 aaagtgacaa aagaaaatat tcagcaaaca aacagtgcga agcaggtgaa aacgcaggaa
      721 gcttatcagg cagaaatcac cagccttaaa agcaacatcc agattaacca gacagaaatc
      781 gccaggcttt taaacaccat ccagggcaca ccgaccttga caactcccat tgcaatgtca
      841 atgtccgctc cgattccgtc tcgtacccca acacctactc ctgccgattc accttatata
      901 tcctttctga atgtaaaaat ccgcgctgtg atgtgcaacg agcagattgt caaagacaat
      961 cgctgtcaag ctcggccagg cgctacgatc aaagtggagc tggaatatat tatcgttgcc
     1021 gaagactatt gtccttattg tattgatgag atccagatcg gtttcagcca tactgacccg
     1081 ctcacctgca tctaccaggg cataccagac cgcaacggga cgcaaggaaa agctgagatc
     1141 gaaattacca tgcccaaaga ggcaggtata tataacctgg cagttgaccg cgcccaggat
     1201 tattcctgcc cgagctggtg gtggaataac agaccgccgg gtgaagacca gtatttcttc
     1261 agaatcgaag tgccttaggt cccgcggctg acaatcatat gtgatcaatt cggagcaagc
     1321 caaagcccga gattatcaac tcaacgaaac aaaattgcag gctgtttaaa cagccacccg
     1381 gcgcgataaa cgctatacgc ctgccaagag cgcgtccaat atctatccga ggagctggct
     1441 tatacgacac caacatcgac accgacaccg actccgaccc catagaagtg accgggaagg
     1501 tcaatcggcg cactggcttc gatcagttat ttatccactc tctacacaaa gtaggttttc
     1561 acgaattgga ccgcctcatc caggtcgtaa atttgaccga cctgctccag ctcggggata
     1621 ctttcgaggc tgtccagccc gatcggcttg ttcaggcgcg ccgcaatgcg gtggtagcgc
     1681 ttccccagga tgctcttgca ctggtagtgg gcgacttcca ggctgccctc cagcatgata
     1741 tcgaccagcg ggcgggccca ctgcgtccag ccccagtccg catcttgggt ggtcaggaat
     1801 ttcgggaagc cgcccgtgcc tactgagagc aaggcgatct ccctcaagcg ttgcttggcc
     1861 gttcccttgt ccaacgcctg ggccattttc gctggatgcg ccatgttcca ttcttggcca
     1921 gggtcttgtg catttaccac tatcagcttg gaggggcaac aagataagga aaatctgcga
     1981 tcaacggttg aaccactatg caagcacctc aatgacagcg ttctccgcgt ctacccgcaa
     2041 cttttcgcct gtccttattt tttcaatgga aatgtgatcc acgcagggta tctcggcgat
     2101 gatgcagccc acagcggtga tcgtctcaca ctcgctattt aggatagcgg caggggcaag
     2161 tccgtttttc ttgaggcgat agagagtata agatcctacc gtcgagcctt ttccagtagg
     2221 gaaggccagc actttgccgg cgatggatag cccttccaac tcatgcccgc gctccactac
     2281 gataccgctc tctgggtcga cgccaccata aaaggagatg cccatggaag taaccagagc
     2341 ctctccctgg gcattgcctg caaagattgt tcttccatgt atcaacatat ttatgccagg
     2401 gggtttatcc gcgaataatg ccaattgcgc aaa
//
LOCUS       DBNGSX010000456         2033 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1567935, whole genome shotgun sequence.
ACCESSION   DBNGSX010000456 DBNGSX010000000
VERSION     DBNGSX010000456.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1567935"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1297)
                     /locus_tag="AC16CB_13560"
     CDS             complement(<1..1297)
                     /locus_tag="AC16CB_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343542.1"
                     /translation="MYEPNLFAEKYQLALETALKEKPQGGVCGFELEWNLLDDQFRPL
                     VTVGSGPSRQSFVDYLRAECLPSWLAAYSQLEVFHWMIEWATRPQYSPKAAVYEGRLM
                     EAALLNGLARAGREFGERLFTWHGNLLYLTPVGKDSIPGSWHIAKRRYLERCVDLYGD
                     ALATAGTHSNLSLPDPLLAWDFMHLPASERNGYSQAGSLPQHFDEYKSQFYITGTRLT
                     RAFAALFIATSASTPIQSQARDGNPVAVLTEIDSVRNLTFPNPSAIDLPDLYRSYNDY
                     LQLSYDLVRRGVRFGNNNWTPVRARSFAEPVERLIAVTSEQLNDLYARGLYALGENLP
                     ADEMARQIEIQNLMARINLPMARVEVRTDDGGHPLEIEVANLTLKHLLLIRFYADPSF
                     ARAFRYDHEDIARARRNEENAARFGLRSEIENPFTGKPVG"
     gene            complement(1326..>2033)
                     /locus_tag="AC16CB_13565"
     CDS             complement(1326..>2033)
                     /locus_tag="AC16CB_13565"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3343543.1"
                     /translation="GRKIAGLVITCPDNPTGLTISADRQVALAKAALESGVAYVLFDW
                     MYHYVTDEQPMNLNTFLSQFDPVERKRLMFLDGITKSLGGSNIRNCHLIATEEVVKFI
                     VARASHTVIPSFYSLAVAMAAYEMGYQNACRGIVEPTNESRKVLKAFLDENHFTYILG
                     KGYYAFIHVGKWLERKGWMDTEPMGQYLAEEHGVALVPGAFFSAYGGEWIRFSYATPP
                     ERTLGAAQRLLQGLEAM"
BASE COUNT          512 a          530 c          483 g          508 t
ORIGIN      
        1 tgccaacagg tttgcccgta aaagggtttt cgatctcgga gcgcagccca aatcgagcag
       61 cgttttcttc attccttcgt gcgcgagcga tatcctcatg gtcatatcga aaagcccggg
      121 cgaatgatgg atcagcatag aaccgtataa gaagtagatg ttttaacgtt agatttgcta
      181 cttcgatctc gaggggatgc cctccatcat ccgtacggac ttcaacgcgc gccatcggaa
      241 ggttaatgcg cgccatcaaa ttctggattt cgatctgcct agccatctca tctgctggca
      301 aattctcccc aagtgcatac aatcctcggg cataaagatc atttaattgc tcgctagtta
      361 cagcaatcaa ccgctctact ggctcggcaa aggagcgcgc acgcacaggc gtccagttgt
      421 tgttgccaaa tcggactccc cgcctcacca aatcatagga tagctggagg taatcattgt
      481 aggagcggta taaatctggc aggtcaatag cgcttggatt ggggaaggtg aggttacgaa
      541 cggaatcgat ctcggttaat actgcaaccg gatttccatc gcgcgcctga gattggattg
      601 gtgtagacgc gctggtagcg ataaataagg ctgcaaaagc gcgggttaat cgtgtaccgg
      661 ttatgtagaa ctggctctta tactcgtcaa agtgctgtgg taagcttccc gcctggctat
      721 atccgtttcg ttcgcttgcc ggtagatgca tgaaatccca tgccaacaag ggatcaggca
      781 aggaaaggtt gctatgtgtg ccggctgtcg ccaatgcgtc accatacaga tccacacatc
      841 gttccagata acgacgcttt gcaatatgcc aggatccagg tatggagtct ttgcccaccg
      901 gagtcaaata caataaattg ccatgccatg tgaataagcg ctcgccaaac tctctgccag
      961 cccgtgctaa cccattcagc aaggcagctt ccattaagcg gccctcatag actgctgctt
     1021 ttggcgagta ttgcgggcgt gtagcccact cgatcatcca gtgaaagacc tctaattggc
     1081 tatacgcagc cagccatgat ggcaagcact ctgcccgcaa ataatccaca aatgactgac
     1141 gactcggacc tgatccaact gtcaccaatg gacggaattg atcatcgagc aagttccatt
     1201 ccaactcaaa gccacacaca cctccctgtg gtttttcttt gagggctgtc tcaagtgcca
     1261 gctgatattt ctctgcaaat aagtttggct cgtacatggt tgaaccccgc gagattacgt
     1321 ggacttcaca ttgcttctag accttgtaat aatctttgag cagcgcctag cgtgcgctct
     1381 ggtggagttg cgtaagagaa tcggatccat tctccgccat acgcagaaaa gaatgcccca
     1441 ggtacaagcg ctacaccatg ctcttccgcc agatattgac ccataggttc tgtgtccatc
     1501 caacctttcc gctccagcca tttgccaacg tggataaaag cataatatcc cttcccaagg
     1561 atgtaggtaa agtgattttc atccaaaaat gctttgagca ctttgcgact ctcatttgtt
     1621 ggctcaacga ttccacgaca agcgttttgg tagcccattt cgtacgctgc catcgctaca
     1681 gctagagaat aaaacgaagg gataacagta tgcgatgcgc gcgccacgat gaacttgacc
     1741 acttcttccg ttgcgatcag gtggcagttg cgaatgttag aaccacccag acttttcgtg
     1801 attccgtcaa gaaacatcag gcgttttcgc tcaacaggat caaattggga taggaacgta
     1861 ttcaaattca ttggctgttc atcagtcaca taatggtaca tccagtcgaa cagcacatag
     1921 gcgaccccgc tttctagtgc cgcctttgcg agagccactt ggcgatctgc tgagatggtt
     1981 aacccggttg gattatctgg gcaggttatg accaagccag cgatcttacg gcc
//
LOCUS       DBNGSX010000457         5009 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1915049, whole genome shotgun sequence.
ACCESSION   DBNGSX010000457 DBNGSX010000000
VERSION     DBNGSX010000457.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1915049"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="AC16CB_13570"
     CDS             complement(<1..365)
                     /locus_tag="AC16CB_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HIR3343544.1"
                     /translation="MSDRLINLIYQEKEAQRLDKFLVSRLPDLSRARIQGLIKDGFVY
                     VDGLEAHKSGQIVDRGSVVRVSIPPPEPTTLEPEAIPLAIVFENQDLIVVNKPAGMVV
                     HPGAGHTRGTLVHAALAHAP"
     gene            complement(358..882)
                     /gene="lspA"
                     /locus_tag="AC16CB_13575"
     CDS             complement(358..882)
                     /gene="lspA"
                     /locus_tag="AC16CB_13575"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR3343545.1"
                     /translation="MRKYLRDYGYLLSLAALIITLDQWTKALVRSNLDFEEIWSPWHW
                     LLPYARIVHWKNTGAAFGMFQGFGGVFTVLAVVVALAILYYFPQVPRQDWTLRLAMGM
                     QLSGALGNLIDRLTQGYVTDFISIGTFPVFNVADSSISIGTAILILGMWYRERHTMPV
                     EPASPISPEEIQGE"
     gene            1090..1656
                     /locus_tag="AC16CB_13580"
     CDS             1090..1656
                     /locus_tag="AC16CB_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014463177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3343546.1"
                     /translation="MDYKKFDDEALLRLVVLAQEQALGALYDRYGRLVYSMALNVTGD
                     PGLAEEITQDVFLRVWNKAETYRAEQGKVITWVSSIARYRAIDLLRRRSIRPEGNIAS
                     WVDDVGFKQPDSVDVEAEIEMAQRRYRVRWAMAQLPPEQRQALAYAYFQGYSHSEIAK
                     RLREPLGTVKTRIRLAMQKLRQLLHEES"
     gene            1759..2475
                     /locus_tag="AC16CB_13585"
     CDS             1759..2475
                     /locus_tag="AC16CB_13585"
                     /inference="COORDINATES: protein motif:HMM:NF052375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HIR3343547.1"
                     /translation="MIEGHVLDLLPAYSLGCLDEGDDLLVAEHLATCKICRAELDTYT
                     VLAADLPLAMNESDPPPDLRDKILQRARAAELPATEAQNPSLWQRLIRQDATPAWSLA
                     SLVLILVLAASNLYLWGRLNSLENRRPDGLMAVQFQGTNAAPNATGLLVISLDGMHGT
                     MVVDQLPVLGEAEQYQLWLIQDGERTSGGVFSVDTQGYKSLYIDSPEPLASYSAFGIT
                     VEPAGGSPGPTGEKVLGGEL"
     gene            complement(2584..3516)
                     /locus_tag="AC16CB_13590"
     CDS             complement(2584..3516)
                     /locus_tag="AC16CB_13590"
                     /inference="COORDINATES: protein motif:HMM:NF048802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7544 domain-containing protein"
                     /protein_id="HIR3343548.1"
                     /translation="MDIGYVLSRAWQIIWKNKVLWVFGILAGCANGGGGGGGGGNVSN
                     TFQRDFPGGQPFERMFENIPDYQIWGLIAIVILVILVLVVLAIFLGTIGRIGLIRGTQ
                     QAEGGATSLVFGELFSGSMPYFWRVFGLSLLVGLVILVAVGLFVLLAFTGTVATLGLG
                     LVCLIPLICILVPVMWFINVIVEQASIAIVLENLGIMDGLKRGWEVVRANLGTMVIMA
                     LILLLGVGGIGGLIIALPILIIVVPAIIGAAVGTDQAIGGGLLLAGICFITYLPVLLV
                     LNGILRSYIGSAWTLTFMRLTGEPASLEPSVEAA"
     gene            complement(3642..4823)
                     /locus_tag="AC16CB_13595"
     CDS             complement(3642..4823)
                     /locus_tag="AC16CB_13595"
                     /inference="COORDINATES: protein motif:HMM:NF016013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HIR3343549.1"
                     /translation="MSLPKTEQFPDDPESLPPARRRRVRRLLAPLNADERADYLDEFA
                     HRASPTFDFFLFSLVSGAVMGIGLLLDAPALLVLGAVLAPLMAPTIGLSLGTVTGSGR
                     YFLRSLLGVLIGGLLASLGGAAAGLATQYWVSPELTQAYFHAQLSWSNFLVLAIGACY
                     TAAAMLHDKHNPALPSVALAYELYIPLAIAGFGLTSGAPHLWPDGLVIFAIHLAWSAL
                     LGTITLAILGFRPLTIFGYTIGGVVTLLVITLLIGLSGAGAILGGQMALPTPVPTATF
                     TITPTLTRTSTPVPPTATLTPTLTFTPTSTATQTVTPTPTPIYAIIQTTDQKGAVLRA
                     QPGGEVLRSYFDGTLMQVLPDQVTIDGVEWVHVIAPDGLEGWMVQRLLATATPAPEWG
                     G"
     gene            complement(4828..>5009)
                     /locus_tag="AC16CB_13600"
     CDS             complement(4828..>5009)
                     /locus_tag="AC16CB_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3343550.1"
                     /translation="GQGANDANMLKEAALGICVLSKEGTAVEALLAADLLAPDIFFAL
                     ELLEKPLRIVASLRR"
BASE COUNT         1173 a         1391 c         1415 g         1030 t
ORIGIN      
        1 ggggcgtgtg ccagggcggc gtgtaccagg gtgcctcggg tatgacccgc cccagggtgc
       61 accaccatgc cagcaggctt attcactacg atcaggtctt ggttttcgaa aacgatcgct
      121 agcgggatag cctccggctc cagggtggtg ggttccgggg gtgggatgct cacccgaact
      181 acgctacctc gatccacaat ctgcccgctc ttgtgtgcct ccagcccatc tacataaaca
      241 aagccatctt tgattaatcc ctggatgcgg gcgcgggaca agtccggcaa gcggctcacc
      301 agaaatttat ccaggcgctg ggcttctttt tcctgatata tgaggttgat aagacgatca
      361 ctcaccctgg atttcctcgg gagatatggg tgaggctggt tcgacaggca ttgtgtgccg
      421 ttctctatac cacatcccca ggattaagat cgctgtcccg atcgaaatgc tggaatcggc
      481 cacattgaaa accgggaaag tgccaatgga gatgaaatca gtcacgtatc cttgtgtcag
      541 cctgtcaatc agattgccaa gggcaccgct aagttgcatg cccatcgcta gacgcagcgt
      601 ccagtcttgg cggggaacct gggggaaata gtacaggatg gccagggcga cgacgacagc
      661 caaaacagta aacacaccgc cgaagccctg aaacatgcca aaagctgccc cggtattctt
      721 ccaatgcaca attcgggcat agggcagcag ccagtgccac ggcgaccaaa tctcttcgaa
      781 gtccaggttg gaacgcacca acgcctttgt ccattggtct aatgtaatga ttagcgcggc
      841 caggctcagt aagtaaccat aatctctcaa atattttctc aaaacaggct cccaagaatt
      901 caaattgtcc ccctcatttt acctcagatg ggttaacgcg attcaatgcc cagggcaatt
      961 gcatatgccc ccaccaaaac accccatttt ctctcctcag tggtgggcaa tcgcccgaat
     1021 gcgattgccc tgattcaatg ccttgataat tgggacacta tgttatactc cctcacttga
     1081 gagatgcagg tggattataa aaagttcgat gatgaggcgt tgctgcgcct ggtggtactg
     1141 gcgcaggagc aggctttggg cgccctctac gaccgctacg ggcggctggt atacagcatg
     1201 gcgctaaacg tcaccggcga tccggggctg gccgaagaga tcacccaaga tgttttcttg
     1261 cgcgtgtgga acaaagccga gacctaccgc gctgagcagg gcaaggtgat cacctgggtg
     1321 agcagcatag ctcgctaccg cgccattgac ctgctccgcc gccgcagtat ccgtccagag
     1381 ggcaatattg cctcttgggt agacgatgtt ggctttaagc agcctgattc cgtcgatgtg
     1441 gaggctgaga ttgagatggc acaacgcagg tacagggtgc gttgggctat ggcccagttg
     1501 ccccccgaac agcgccaggc gctagcctat gcctatttcc agggctactc ccatagcgag
     1561 atcgctaaaa ggctgcgtga accactgggc actgtaaaaa cccgcatccg cttggctatg
     1621 cagaaattgc gccagttgtt gcatgaggag agttaaccgg atttataaat ccgatttcaa
     1681 ggttaaatcg tattaataaa aagatacgtt gcaaaaaaca gcgcccccaa atatcgggcg
     1741 cttgagtcga gttgagattt gatagaagga cacgttttag atttgctacc agcgtactcg
     1801 ctggggtgtt tggatgaggg agatgacctg ctggtagccg agcacctggc tacctgcaag
     1861 atctgccgcg ccgaactgga tacatacacc gtgctggcgg ctgatctgcc tttggctatg
     1921 aacgaaagcg acccgccgcc cgatctgagg gacaagatct tgcaacgcgc ccgggctgcc
     1981 gaactgcctg caactgaagc gcaaaatcca tccctgtggc agcgcttgat ccggcaggat
     2041 gctactccgg cttggtcgct ggcaagcctg gtcttgattt tggtgttagc tgccagtaac
     2101 ctgtacctgt gggggcgtct gaattcgctt gaaaatagga gaccagacgg attaatggcg
     2161 gttcagttcc aggggacaaa tgcggcgcca aatgcaactg gcctgctggt gatcagcttg
     2221 gatggtatgc atggaaccat ggttgtggat caactgcctg tcttgggcga ggccgagcaa
     2281 tatcagctct ggctgatcca agatggtgag cgaaccagcg gaggcgtgtt ctctgtagac
     2341 acccaggggt ataaatcgtt gtatattgac tccccagaac cactggcgtc ttactccgcc
     2401 ttcgggatta ccgtcgaacc ggctggtggc agccctggtc cgacgggtga gaaagtgctg
     2461 ggtggagaac tgtaaccccc gatcaaatat ttaaaaagac ctgcgtgcgg gctataaccc
     2521 gcacgcaggc ttttaagata ttattccaca gctaagcata gccgccggga ctaggtgcag
     2581 tcgttacgct gcctcgacag aaggctccag gctggctggc tcacccgtca ggcgcatgaa
     2641 agtgagcgtc caggcagacc caatgtagct gcgcaagatg ccattcaaga ccaggagaac
     2701 aggtagatag gtgatgaagc atatcccggc caatagtaaa ccgcctccga tagcctgatc
     2761 tgtgcccaca gccgcgccaa tgatcgctgg cactacaatg atcaggatcg gcagggcgat
     2821 gatcaacccg cctatgccac ccacacccag cagcaggatg agcgccatga taaccatcgt
     2881 ccccaggttg gcgcgcacca cctcccagcc tcgttttagc ccgtccatga tgcccagatt
     2941 ctcgagcaca atggcgatgc tggcttgctc aacgatcacg ttgataaacc acattaccgg
     3001 cacaaggatg cagatcaagg gaatgaggca cactaggcca agtcccaatg tggccacggt
     3061 ccctgtgaaa gcaagcagca caaacaaccc taccgctacc aggatcacca ggcccactaa
     3121 caggctcagg ccaaagaccc gccagaagta gggcatgctg ccactgaaca gctctccgaa
     3181 gaccaggctg gttgcgccac cctccgcctg ctgggtgccg cggatcaggc cgatgcgccc
     3241 gatcgtgccc aagaaaattg ccagcaccac cagcaccagg atcaccagga tcacgatggc
     3301 aatcagcccc caaatctggt aatcagggat gttttcgaac atcctttcaa aaggttgtcc
     3361 tccagggaaa tccctttgga aggtgttgct aacgttgccg cctccccctc cccctccacc
     3421 gccatttgcg cagccagcca ggatgccgaa gacccacagc actttgttct tccagataat
     3481 ttgccaggct cttgagagta cgtaaccgat atccattttg cattctcctt gaactgtgta
     3541 acctctgact ttgatagcaa tcaatccatc agtattattg tatacgattt acatggagat
     3601 aggtttcatg ccaggataac tcataacgtg atgtataact ttcaacctcc ccactcggga
     3661 gctggcgtgg cagtcgccag caggcgctgc accatccaac cttccagacc atctggagca
     3721 atgacgtgca cccactccac cccgtcaatg gtcacttggt ctgggagaac ctgcatgagc
     3781 gtcccgtcga aatagctgcg cagtacctcg ccgcctggct gggcgcgcag cacggcgcct
     3841 ttctgatccg tcgtctggat aatggcgtag atcggggtgg gtgtgggggt gactgtttgg
     3901 gtcgcagtac tagttggggt gaatgtgagc gtgggagtga gcgtggcggt tggaggtaca
     3961 ggcgtggaag ttctggtgag cgtgggggtg atggtgaacg tcgcggtggg tacgggtgtg
     4021 ggcagggcca tctgcccgcc caggatcgcc cccgcgccgc tcaggccaat cagcagcgtg
     4081 atgaccaaca aggtgaccac tccaccaatc gtgtaaccga aaattgtcag cgggcggaaa
     4141 cccaagattg ccagtgttat agttcctagt agggcgctcc aggccaggtg tatggcaaag
     4201 atcaccagac catcgggcca gaggtgcggc gcgccgctgg tcagtccgaa gccagcaata
     4261 gctaaaggaa tgtagagctc gtaagccagt gccacgctgg gcaaggcggg attatgtttg
     4321 tcgtgcagca tagccgccgc ggtgtagcat gctccgattg ccagcaccag gaagtttgac
     4381 caggataatt gggcgtggaa atacgcctgg gttaattctg gtgaaaccca atattgggta
     4441 gccagtccgg cggccgcgcc ccccaggctg gctagcaggc caccgatgag cacgcccagc
     4501 aagctgcgta ggaaatagcg ccccgagccg gttaccgtgc ccaatgaaag gcctatggtg
     4561 ggcgccatca agggggccag caccgcccct aacaccaaaa gtgccggggc atctagcagc
     4621 aagccgatcc ccatcactgc gccggatacc agggagaaca ggaagaaatc aaaggtcggc
     4681 gaagcgcgat gggcaaactc atcgagatag tcagcgcgtt catcggcatt tagaggagcc
     4741 aacaagcggc gcaccctgcg gcggcgggca ggtggcaggg attctgggtc gtcaggaaac
     4801 tgttctgtct tgggtaagct cataggttta acggcgaagg ctggctacga tgcgtaaagg
     4861 tttttcgaga agctccaaag caaagaagat atctggggcg agcaggtcgg ctgccagcaa
     4921 ggcctccaca gctgtgccct ctttagacag cacgcagatc cccaaagcgg cttccttcag
     4981 catgttggcg tcgttggccc cctgcccaa
//
LOCUS       DBNGSX010000458         4061 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1638178, whole genome shotgun sequence.
ACCESSION   DBNGSX010000458 DBNGSX010000000
VERSION     DBNGSX010000458.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1638178"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..2364
                     /locus_tag="AC16CB_13605"
     CDS             205..2364
                     /locus_tag="AC16CB_13605"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006825265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0043682 - P-type divalent copper
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HIR3343551.1"
                     /translation="MDHHDMSMKNPNEEHRDNAGHQEMVAHDEHQAHAQETIVQVEME
                     NHAGHEGMDHASAGRQQGHVDHTGHEGMFRQRFWVSLALSIPVLLYSPMIQRWLGFSM
                     PGFPGSQWITPLFSVVVFVYGGIPFLDMARPELRNRRPGMMTLISLAISVAFVYSLGA
                     LFVLEGDTFFWELVTLIDIMLLGHWLEMRSVRQASGALNELAKLMPDTAELIRVDGSI
                     TKVQVAKLRHGDMVLVRPGSSIPADGVVEEGDSEVSEAVITGESRPVNKEPGDQVIAG
                     TINGDGSLRVRVTATGDETTLAGIMRLVEQAQQSKSTTQILADKAAGWLFYIALGVAA
                     VTGIAWSVAVGFNVEVIARVATVLVIACPHALGLAIPLVVAITTAIGAKNGILVRDRL
                     ALEQARQIDTVIFDKTGTLTRGEFGVVDIVTSDGWDENQALALAAAIEGDSEHTIARG
                     IRRTAEERGIHLPKVNDFEAIKGRGVKAKHDGQSVYVGGPRLLEMLQFKLPEQIAGFE
                     EQANQKGYSLVNLVVNSEVVAGFALADVIRPESKQAIQKLHDMGVQVAMLTGDSQAVA
                     KSVAEELGIDQYFAEVLPEHKDQKVTELQRQGKRVAMVGDGVNDAPALTRADVGIAIG
                     SGTDVAVESAGIILVKSNPLDVVKIFSLSRASYRKMIQNLLWATGYNVVALPLAAGIL
                     APFGILLSPAVGALFMSLSTVIVAINAQLLRRVDIRG"
     gene            2366..2944
                     /locus_tag="AC16CB_13610"
     CDS             2366..2944
                     /locus_tag="AC16CB_13610"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3343552.1"
                     /translation="MSSSTLSRSSNCGRTRSKFGFYGLLLVAGLLTTACGMFWRFPQF
                     FQSLPASLGGDESVSNGERIYFTATNVDGDWVRYSGGPRFGGMMMGSYLTCAACHGPE
                     GRGGIHTMHMQVMDAPDIRYVALSEEAGEHKDEQQDDHSDAHGEYTLDDFRLAVVEGE
                     HPDGQPLSRDMPRWRMSEKDLADLFEFLKSLP"
     gene            3389..>4061
                     /locus_tag="AC16CB_13615"
     CDS             3389..>4061
                     /locus_tag="AC16CB_13615"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3343553.1"
                     /translation="MELKTDRMQSARYALLNDVVLLLTDTPDWQHFLQTFISKMREGL
                     DFNYSTIAWLNRESQTYKLQTVFEQGKYVLHMDKADISVEQDIPGAVIRSRQARLVND
                     ISALEDGATSPDDLAFGDGSLSALIALPIQVQDKVFGAITFATARKDGYTDEDLHFAS
                     SMAAFLALEIDRGQQAQQLKLTREELARLSSFPELNPAAIIELDSHGQIYYMNPAAAE
                     QFPEWQ"
BASE COUNT          988 a          944 c         1176 g          953 t
ORIGIN      
        1 ttaccctcac ccagttaatc tataggggga gcaccttctt tacctcagcc aggttgatgg
       61 atataattca acaggctaag gtattttgcc tattgacaat aagccatttt gcgcttgtcc
      121 gggtagatga atattcttac aatatcgatg cgtaaaaagt ctcaatgagc gggcagtgcg
      181 aatgttgccc ggaagggtag taatatggac caccacgata tgagtatgaa gaatccgaat
      241 gaagaacatc gcgataatgc tggacaccag gagatggtgg ctcacgatga acatcaagcg
      301 cacgctcagg agaccatcgt tcaggtcgag atggaaaacc atgctgggca tgagggcatg
      361 gatcacgcat ctgcagggcg ccagcagggt catgtcgacc acacaggaca tgaggggatg
      421 ttccggcagc ggttttgggt ctcgctggcg ttgtccatcc ctgtgctgct gtatagcccc
      481 atgatccaaa gatggcttgg attcagcatg ccaggttttc ccggtagcca atggatcacg
      541 ccgctcttct cggtggttgt ctttgtgtac ggcggcatcc catttctgga catggcgaga
      601 cccgaactgc gcaatcgccg cccggggatg atgaccttga tctcgctggc aattagcgtg
      661 gctttcgtat acagcctggg agccctattt gtacttgaag gggatacctt cttttgggaa
      721 ttagtgacgc tgatcgatat tatgttgctc gggcactggc tggagatgcg cagcgtgcgc
      781 caggcctccg gcgcattgaa cgagctggcc aagctgatgc ccgacacggc tgagctcatc
      841 cgtgtggacg gcagcatcac aaaggtccag gtggcgaagt tgcgtcatgg tgacatggtt
      901 cttgtgcgcc ccgggagcag tattcccgct gatggagttg tggaggaggg tgattcagag
      961 gtaagcgaag ccgtgatcac tggagaatcc aggccggtaa ataaagaacc gggagaccag
     1021 gtgattgccg gaacgatcaa tggcgacggc agtctgcgcg tgcgggtgac cgccacgggc
     1081 gatgagacta ccctggcagg tatcatgcgc ttggtggagc aagctcagca aagcaaatcc
     1141 accacccaga tcctggcgga taaagcggca gggtggctgt tttacattgc cctgggtgta
     1201 gctgctgtaa ccggcatagc ctggagcgtt gcggttggat ttaatgtgga agtgatcgcc
     1261 cgggtggcga ccgttttggt gattgcctgc cctcacgcct tgggtctggc gatcccgctg
     1321 gtcgttgcca tcacaaccgc cattggcgct aaaaatggca ttctggttcg tgataggctg
     1381 gcgcttgagc aggcgcgcca gatcgacaca gtgatctttg ataaaacggg tacgctgacg
     1441 cgtggggagt tcggcgtagt cgatattgtg accagtgatg gctgggatga gaaccaggcg
     1501 ctagcgttgg cagctgcaat tgaaggcgat tctgaacaca ccattgcacg tggcattcgg
     1561 cggactgccg aagaacgtgg gatccacctg ccaaaggtga acgacttcga agccattaag
     1621 gggcgtgggg tgaaagccaa gcatgatggg caatctgtct acgtgggcgg gccgcgattg
     1681 ttagaaatgc tgcaattcaa gttgccagag cagatcgctg gatttgagga gcaggccaat
     1741 caaaaaggct atagcctggt taacctggtg gtaaatagtg aagtggttgc cggattcgcc
     1801 ctggcagatg tcatccgtcc ggagagcaag caggcaatcc agaaactaca tgacatgggc
     1861 gtgcaggtcg ccatgttgac cggcgacagc caagcggtgg caaagtctgt ggcggaagaa
     1921 ttgggcatag accagtactt tgccgaagtt ttacctgaac acaaggatca aaaggtgacc
     1981 gaattgcagc gacagggcaa gcgggttgcc atggtgggcg atggggtgaa cgatgctccc
     2041 gccttgaccc gggctgatgt gggtattgcc attggcagcg ggacggatgt ggcggtggaa
     2101 tcggctggta tcatcctggt gaagagcaat ccgctagatg tggtcaagat tttctcgctg
     2161 agccgggcga gctaccgcaa gatgatccag aacctgcttt gggcgacggg ttataatgtg
     2221 gttgcattgc cccttgcagc gggcatactg gcgccttttg gcatcttgct ttcaccggca
     2281 gtaggagccc ttttcatgtc attgagcacg gtaatcgtgg cgatcaatgc ccagttgctg
     2341 aggcgggtag atatcagagg ataaaatgag tagttccacc ttgtcccgct cctcgaactg
     2401 tggaaggaca agatcaaaat tcggcttcta tggattgctt cttgttgcag gattgctgac
     2461 cactgcctgt gggatgtttt ggcgttttcc tcagtttttc cagtcacttc cggcatcgct
     2521 gggcggagat gagagtgtgt ccaatggaga gcgcatatac ttcactgcga ccaatgtaga
     2581 tggagattgg gtcagatact cagggggtcc aaggtttggc ggcatgatga tgggatcgta
     2641 tttaacctgc gctgcctgcc atgggccaga aggacgcggg ggcattcata cgatgcacat
     2701 gcaggtgatg gacgcacccg atattcgtta tgtggcgctg agcgaagaag cgggcgagca
     2761 caaagatgag caacaagatg atcatagtga tgcgcacgga gaatatacat tggatgattt
     2821 ccgcttggca gtggtagagg gggaacatcc ggatggccag ccgctcagcc gggatatgcc
     2881 gcgatggaga atgagtgaaa aggacctagc cgatttattt gaattcttaa aatcgctacc
     2941 ttaaaagaac ttacggggtt tgatggcggg cacgcccagc tcatatttct taagtctata
     3001 atgagaaatc agccacacgt aattgtataa ttaaggtaaa tattatataa taattgtggt
     3061 ggacaaagat tgttcgcagt tgaaattgtt gccctccatt ttgaccgaat atcaagccag
     3121 ttactggctg agcccaacgg ctgatgaata gataggcaaa gcgcttcatc gaccaacatc
     3181 gggctatcca gatcattcag caagtcaagc gcaggggcaa aattcgacag attacgatcc
     3241 attggaagat accctagata ggtcacatcc cattttgcgt aacttctcta tcattgtttt
     3301 gactacgcag ccaacgataa ggtaccagca acatcattgg caatcatgga tattgcctta
     3361 tggagccgat tgacaagcag aggcgaagat ggaactaaag actgaccgga tgcagtctgc
     3421 acgttatgca ttgctaaacg atgttgtgct gctgctaacc gatacacccg attggcagca
     3481 ttttttacaa acctttatca gtaaaatgcg agaaggcctt gattttaatt acagcacaat
     3541 tgcctggctc aatagggaat cccagactta taaattacag acagtgtttg agcaggggaa
     3601 atatgttctc cacatggaca aggcggatat ctctgtggaa caggatattc ccggcgctgt
     3661 catccgtagc cgccaggcgc gcctggtcaa tgatatttcc gcccttgaag atggggcaac
     3721 ttcaccagac gatctggcat ttggggatgg gtcgctatcc gctttaatag cgttgccaat
     3781 tcaagtacaa gataaagttt tcggcgccat cacttttgcc acagcccgga aagacgggta
     3841 cacagacgaa gaccttcact tcgcttcttc tatggctgct tttcttgccc tggaaatcga
     3901 ccgcgggcag caagcccaac agttgaagct caccagggaa gaactggctc gcctgtcctc
     3961 attccctgaa ctgaatccag cggcgatcat cgaattagat tcgcatggac aaatttatta
     4021 catgaacccg gctgccgctg aacaattccc cgaatggcaa c
//
LOCUS       DBNGSX010000459         6273 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1153301, whole genome shotgun sequence.
ACCESSION   DBNGSX010000459 DBNGSX010000000
VERSION     DBNGSX010000459.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6273)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6273)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6273
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1153301"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1440
                     /locus_tag="AC16CB_13620"
     CDS             <1..1440
                     /locus_tag="AC16CB_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343554.1"
                     /translation="LAGFDLPYNILLLSWEPPELFSASPLLLVDKISWPFALGLATLA
                     LAVIVTEVAREFEAELSSWAASLALAALGMTAVLAGNPLTLLLGWSALDFIELFILLR
                     QLQRTEERRQVVNAFAARVAGTVLLLMGLILASASNIKLSFENVPTNVSIYLLLGASL
                     RLGVIPVHLPFWREPPLRRGIGTISRLTTVAASLVLLPRVASAGVQQEQAAILLLLTS
                     LAAIYSGYSWFNSTNELEGRPFWILGLAALSLAAAIRGQPLASLAWGSALIFTGGLQF
                     LYSVRSRYLLVLPLLSVFSSASLPFTPTWYAGQIYSSPYSVWQIVFIISQALLLGGVV
                     RHSMRSVGELSRVERWVWVIYPIGLIMILFSQYLASWLGGTLLGGAGWEIGFGTMLPG
                     IMGLVLTGLWLLWARKIPRVPKQVVAIFDKVFSLGWFYFLLAWLFRLTGQALSFITAV
                     LEGEGGILWALLLLVLLIAFLSQRGFSGG"
     gene            1444..2088
                     /locus_tag="AC16CB_13625"
     CDS             1444..2088
                     /locus_tag="AC16CB_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343555.1"
                     /translation="MELINFHNFAMPGALLVLITTMLLLTVRDWRVSLVLLAVQYIGV
                     YVLVAIHWPMMIALTKLVAGWIAAAVLGTAMSSADHAALETDRSQNKHSVFRWITGLS
                     FSAWVFRMLTAAMIVLVVFTLAPLTAEWIPTIQFEEVIGALILLCLGLLHLGLTAYPL
                     RVVIALLTTLSGFEIIYAAVEASTLVAGLLAVVTMGLALIGAYFQVASSREVEI"
     gene            2085..3512
                     /locus_tag="AC16CB_13630"
     CDS             2085..3512
                     /locus_tag="AC16CB_13630"
                     /inference="COORDINATES: protein motif:HMM:NF012581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter transmembrane
                     domain-containing protein"
                     /protein_id="HIR3343556.1"
                     /translation="MTSPLIWIVLPAVYAGILFIVRRWERTVVILGILISLLLAWFAW
                     VAPIGETMVVGPWRANLDDTLTILGRRFVLDAADAPVLTLIYLGLAFWFGGSIIANTG
                     NLFVPIGLANAALLTAAIAVQPFLYSALLIELVTIISIPLISPPNKPVERGTIRFLTF
                     QTLAMPLILFTGWILTGVESGPPDSVFVVHGNILLALGFAFLLAIFPFHTWVTMLAEE
                     THPYVGAFIFYTFSLVITFFGISFLERYAWLRTSTGLYDLLRVVGILMVAIAGFWVAF
                     QKHLGRMLGYAVLMETGLSLLILSAGTAGEHLPASLGILFASILPRGLAFAVWALALS
                     TLMGSGNESNTPALSLHLGAVQGRGRKLPVTVVVLVAAMFSLAGFPLLAGFPVRLALW
                     EIVAKQSSLAGLVALLGGVGLLTSGLRTLGILISGADEELWLVKESRGELVLMILGGL
                     GLLLVGVFPQWFLPALANLADIFFG"
     gene            3650..4828
                     /locus_tag="AC16CB_13635"
     CDS             3650..4828
                     /locus_tag="AC16CB_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343557.1"
                     /translation="MEIEQVQKQLNWLDEEHRKAKTTLDLQAKRITSLEGELSPFSQQ
                     IKDLNTEITRLAGILARIDQFDESILQLRIEAKQSIDALEKQLKKREDEIEKVRRVEM
                     RALDDNIDEIRKDISAIGEIKRGMQARIDEEMRLGKSIDEVRLRIDTIRRSEEEYTRS
                     FRLLEDGRRQDAKRLSDLQGESTALRKHMDDLRGKMDLTSTSLRKVETRLNEVSMLEV
                     ERREAQTKFLENQSLLQVERDRVWKDWQARFEVIEKQTSEVESNLQSMDATHREVKRS
                     QKTLEELNQLFERRINEMTEIQRLSEERFRQEWVTFKADDQKRWTNYTLTQEEQRSES
                     QRQYSKLSERVTQIEDHIQEIQDVIQQMNEQTEKRLQSLLANVHEWVSAYERTLGRAR
                     "
     gene            4835..5704
                     /gene="selB"
                     /locus_tag="AC16CB_13640"
     CDS             4835..5704
                     /gene="selB"
                     /locus_tag="AC16CB_13640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HIR3343558.1"
                     /translation="MRVIGTAGHVDHGKSTLVEALTGTHPDRLKEEREREMTIDLGFA
                     WLTLPNGEDVGIVDVPGHRDFIENMLAGVGGIDAALFVIAADEGVMPQTREHLAILDL
                     LQVQGGVIALTKVDLVEDEDWLSLVEDDVRQVVAGTVLGEAPIIRVSARKKIGLETLL
                     NAVGANLEEKPPRLDLGRPRLPVDRVFTISGFGTVVTGTLMDGCLRVGEEVEILPRGI
                     KGRVRGLQTHKKKEDVAIPGSRTAANITSVTVDQIRRGDVVAHPKDYSTTARMDVYFH
                     LLPDSSQSVRHNT"
     gene            5744..>6273
                     /locus_tag="AC16CB_13645"
     CDS             5744..>6273
                     /locus_tag="AC16CB_13645"
                     /inference="COORDINATES: protein motif:HMM:NF051647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA-binding winged helix domain-containing
                     protein"
                     /protein_id="HIR3343559.1"
                     /translation="MRLLGRDELKPGETGWLQLELSEPVVAVRSDRYILRRPSPGETL
                     GGGSVVDPHPKGRHRRFSKDILARLEALAQGTPADIMMQALVAIGAGPWKDVIARSNL
                     NHAQVEAALDELRSTGALIVLDGASDGGVIQNESLVTSKGYWEQLSKRALQELEQYHQ
                     VNPLRRGMPREELKSRL"
BASE COUNT         1406 a         1393 c         1745 g         1729 t
ORIGIN      
        1 cttgcaggtt ttgatctgcc atacaatatt ttgttattaa gttgggaacc accggagtta
       61 ttctctgctt cgcctttatt attggttgac aaaatctcct ggccattcgc tttgggtttg
      121 gcaacactag ctttggcggt aattgtcaca gaagtcgccc gagagtttga agcagaactc
      181 tcgagctggg cagctagtct tgcgcttgct gcattgggta tgaccgcagt ccttgcagga
      241 aatccgttga ctttgttgct aggatggtct gcactggact ttatagagtt attcatttta
      301 cttcgtcagt tgcagcgtac tgaggagcgg aggcaggttg tgaacgcttt tgcagcccgc
      361 gttgccggaa cagtattgct cctgatgggt ttgatactag ccagcgcaag taatatcaag
      421 cttagttttg agaatgtccc aacaaacgtg agcatttacc tgctcttggg agccagcctt
      481 cggttaggtg ttataccagt acatctgcct ttctggcgtg agcctccgct acggcgtggg
      541 ataggaacta tttcccgttt aacaacggtg gcagccagct tggtattact gccacgcgtt
      601 gcgtctgcag gtgttcaaca ggaacaggct gcaattttgc tcctattaac aagcttggcg
      661 gcgatctatt caggttattc gtggtttaac tcgacgaacg agttagaggg taggccattt
      721 tggatcttgg ggctggctgc tttatccttg gcagccgcca tccgtggcca acccctggcg
      781 agcctagcct ggggttccgc attaatcttc acgggaggcc ttcaattctt gtattcggtt
      841 cggagcaggt acttattggt tttacctctg ctcagcgtgt tcagttcagc ctctttgcca
      901 tttaccccta cctggtatgc cggccaaatt tattcatccc cgtactccgt ctggcagatt
      961 gttttcatca tatctcaagc cttgcttcta ggtggcgttg tccgacatag catgcggtcc
     1021 gtgggtgaac taagccgtgt ggaacgttgg gtctgggtga tctatccaat cggattgata
     1081 atgatactct tttctcagta cctggctagc tggttgggag gcaccctgct tggaggggct
     1141 ggctgggaaa ttggttttgg caccatgttg cccggcatca tggggttggt attgacgggc
     1201 ttgtggctac tatgggcgcg taagattccg cgcgttccaa agcaagtagt ggcaatattc
     1261 gacaaagtat tctccttggg gtggttttac ttcctgcttg cgtggctatt ccgcctcact
     1321 ggacaggcgt tatcatttat aaccgctgtt ttagaggggg aagggggtat tctttgggcg
     1381 ttgctgctcc tggtgctact catcgcgttt ctgtcccagc ggggattttc gggggggtga
     1441 tgtatggaat taattaattt tcataatttc gcgatgcctg gagctctgct tgtcttaata
     1501 actacaatgt tacttttgac agtacgggat tggcgggtct cattggtttt actggctgtg
     1561 cagtacatag gggtatatgt actggtggct atccactggc ctatgatgat cgcgttgaca
     1621 aagctggttg caggttggat agcggctgcg gtgttaggta cagcaatgtc tagcgcagat
     1681 catgcagctc tagaaacgga tcgatcacaa aataaacatt ccgtctttag atggataact
     1741 ggcctatcct tctctgcatg ggtatttcgg atgctcactg cagctatgat cgtactggtc
     1801 gtcttcacac tggctccatt aacggcggag tggatcccga ctatccaatt tgaagaggtc
     1861 attggcgcgt tgatattgct ttgcctgggt ttactccatc ttggattaac cgcctatccg
     1921 ttgcgcgttg tcatagccct tctgacgact ctatcggggt tcgaaattat atatgctgcc
     1981 gtggaggctt ccactctcgt ggctggattg ctggcggttg tgacgatggg acttgcgcta
     2041 attggtgctt actttcaggt ggcttcatca cgcgaggtgg aaatttgacc tctccgctca
     2101 tttggattgt tttaccggca gtctacgcag gtatcctttt catcgttcgt cgatgggaga
     2161 ggacggttgt catccttggc atcctgattt cgctactttt ggcgtggttt gcctgggtcg
     2221 caccgatcgg tgagacaatg gtggtaggtc catggagagc aaatctcgat gatactctta
     2281 ccattttagg tcggcggttt gtacttgatg ccgctgatgc acctgtattg acattgattt
     2341 atttgggctt agctttttgg tttggtggtt ccataatcgc gaatacaggt aacttattcg
     2401 ttccaattgg actggcgaac gctgctctcc tgacggcagc catcgctgtg cagccatttt
     2461 tatactcggc attattgatt gaactggtta caatcatcag tattccatta atttcacccc
     2521 caaacaaacc ggtagagcga ggcacaattc gctttttaac ttttcagaca ttggccatgc
     2581 cgctcatttt gtttactgga tggattctga ccggggtcga atctggccct cctgattcag
     2641 ttttcgtggt gcacgggaat attttgcttg cccttggctt tgcgttttta ttggcaatct
     2701 ttcctttcca cacctgggtt accatgctag cggaggagac ccacccttat gtcggggcat
     2761 ttattttcta cactttttcg ctggtgatca ctttcttcgg gatcagcttc ctagaacgtt
     2821 atgcctggtt gcgtacatcg acggggctct atgatttact ccgtgtggtt ggtatcctga
     2881 tggtggcgat agctggcttt tgggtagctt tccagaaaca tttggggcgt atgttgggct
     2941 atgcagtatt gatggaaact ggcctctcct tgctcatcct aagcgctgga acggcaggtg
     3001 agcacttacc agcttcactg gggattctat ttgcatccat actacctcgt ggattggcat
     3061 ttgctgtgtg ggctttggcg ctatcgactt tgatgggatc tgggaatgag tcaaacacac
     3121 ccgccttatc tctccactta ggagctgtgc aaggtagagg aaggaagttg cccgttactg
     3181 tcgtggtatt ggttgcagca atgttctcgc tagccggctt tcccttgttg gcgggttttc
     3241 cggtgcggtt ggctctatgg gaaatcgtcg caaagcaatc atctttggca ggtcttgttg
     3301 ctctattggg aggtgttggt ctgttgacta gtggattgcg cacgcttgga attttaatct
     3361 ctggtgccga tgaagaactt tggttagtga aagagtcgcg gggtgagctg gtgttgatga
     3421 tcttgggagg gctgggattg ctcttggttg gtgtctttcc ccaatggttt ctgcctgcct
     3481 tggcaaacct ggctgatatt ttctttggat agctgcctcg gggtttatac gcatcagttt
     3541 caaccccacc aattaccctt taactgcaaa aaccctttgg aaattgccat gctataatca
     3601 gaccttaggg aggacagagc caaagaattc acttggaggt gatgtctcta tggagataga
     3661 acaagttcaa aaacaactga attggttaga tgaagaacac cgcaaagcga agaccactct
     3721 tgatctgcaa gcaaagcgca tcacttctct ggaaggtgag ctttcacctt tctcacaaca
     3781 gatcaaggat ttaaataccg agatcactcg actggccggg atattggctc gcatcgacca
     3841 gtttgatgaa tcaattttgc aattgcgtat cgaggcaaaa cagtcaatcg atgccttaga
     3901 aaagcaactt aagaaacgcg aagatgaaat tgaaaaggtg cgccgggtgg agatgcgtgc
     3961 tttggatgac aacatcgatg aaatccgaaa ggatatttct gcgatcggtg agatcaagcg
     4021 cggtatgcaa gctcggattg atgaagaaat gcgattagga aaatcaatcg atgaagtgcg
     4081 gttaagaatc gatacaattc ggcgcagcga agaagaatac actcgttcat ttcgcttatt
     4141 agaagacggg cgccggcaag acgcaaaaag gttatctgac ttgcagggtg aatcaactgc
     4201 gctgcgtaag catatggatg atttacgtgg caaaatggat ctgacctcca caagtctgcg
     4261 caaggttgaa acccgtttga acgaagtgtc aatgctggaa gtagagcgtc gcgaagcgca
     4321 gacaaagttc ctggagaacc agtcgctttt acaggtggag cgggatcggg tatggaaaga
     4381 ttggcaggcg cgctttgaag tgattgaaaa gcaaacctcg gaggtggaat ccaatttgca
     4441 gtcaatggat gccacacatc gcgaggtgaa acgatcccag aaaacactgg aagagctaaa
     4501 tcaattattc gagcggcgta tcaatgagat gacggaaatc cagcgcctat ctgaagaacg
     4561 ttttcggcag gaatgggtca cattcaaagc agatgatcag aagcggtgga ctaactatac
     4621 cctcacccag gaggagcaac gtagcgagtc gcaacgacaa tactcaaagc tttcggagcg
     4681 tgtgacgcaa attgaggacc acattcagga aattcaggat gtaatccaac aaatgaatga
     4741 gcagaccgag aaaagattgc agtccttgct ggctaatgtt catgaatggg tttctgcgta
     4801 cgaacgcaca ttagggagag cgcgctagag aagtatgagg gtaattggca cagcgggtca
     4861 tgtagaccac ggtaaatcaa ccttggtaga agcgctcacc ggcacacacc ccgaccggct
     4921 taaagaagag cgcgaacgcg aaatgaccat cgatcttggt ttcgcctggt taaccttgcc
     4981 caatggcgag gatgtgggca tcgtggatgt gcccgggcat cgcgatttca tcgagaacat
     5041 gcttgctggt gtaggaggga ttgatgcggc gctatttgtt atcgcagcag atgaaggggt
     5101 tatgccgcaa acgcgagagc atttggctat tctagatctg ttgcaggtac agggcggggt
     5161 tatcgcgcta actaaggttg acctggtcga agacgaagat tggctgtcac tagtcgagga
     5221 cgatgtacgc caggtggtgg cgggaacggt tcttggcgaa gccccaatca ttcgcgtctc
     5281 ggctcgaaaa aaaattggcc tcgagacgct cttgaatgct gttggtgcga atttagagga
     5341 aaaaccgcct cgtctggatc tggggcgacc tcgcttgccg gtcgaccgcg ttttcaccat
     5401 ctccggtttt ggtaccgtag tcacgggcac cttgatggat ggctgtttac gtgtgggtga
     5461 agaggttgag atactgccac gcgggataaa aggtcgtgtg cgtggattgc agactcataa
     5521 gaagaaggag gatgtagcta tcccaggttc gaggacggcg gcaaatatca ctagtgtgac
     5581 cgtggaccaa atccgccggg gtgatgtggt agcccaccca aaagattact ccacgacggc
     5641 acgcatggat gtgtattttc atttgcttcc cgattcgagc caatctgtgc gacataacac
     5701 ctaagtcaag ctattcatcg gggcgtctga agtagttagc cgtttgcgct tgctgggaag
     5761 ggatgagctg aagcctggtg agacgggatg gttgcaactt gaactctccg aaccggtggt
     5821 agcggtacgc agcgaccggt atatcctgcg ccgtccttca cctggggaaa cgcttggagg
     5881 ggggagtgtg gttgatcctc atcccaaagg tcgccatcgg cgtttctcta aagatatact
     5941 cgctcgcctg gaagcgctag cccaggggac tccagcggat ataatgatgc aagccctggt
     6001 ggcaattggt gctggaccat ggaaagatgt gatcgctcgg tccaacttga atcatgccca
     6061 ggtggaggcc gcgcttgatg agttgcgctc cacgggggcg ctcattgttt tagacggcgc
     6121 ctccgacgga ggtgtaattc aaaatgaaag cctggtgaca agcaaaggct attgggaaca
     6181 gctttctaag cgcgctctcc aggagttaga gcaataccat caagtgaatc cgttgcgacg
     6241 cggcatgccg cgcgaggaat taaagagccg tct
//
LOCUS       DBNGSX010000460         6266 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1084564, whole genome shotgun sequence.
ACCESSION   DBNGSX010000460 DBNGSX010000000
VERSION     DBNGSX010000460.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6266)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6266)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6266
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1084564"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="AC16CB_13650"
     CDS             <1..576
                     /locus_tag="AC16CB_13650"
                     /inference="COORDINATES: protein motif:HMM:NF013811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343560.1"
                     /translation="ISLKTSDDLHSGNAISADFTAQSWHTHLNLPDTPVISAEEAGAR
                     LAKLAVDHDFSMFEFWLSDYAGHHQDMSAACSLLETFDQVIGGLLDTWDDHSGLILIT
                     SDHGNLEDLSTRRHTFNPVPALLIGASELRSSFVHLAKDSAKRHSRGGSSHGSTPYPD
                     KISLNLTNVAPAILGFLGEHHSQRSDLAISD"
     gene            940..1806
                     /locus_tag="AC16CB_13655"
     CDS             940..1806
                     /locus_tag="AC16CB_13655"
                     /inference="COORDINATES: protein motif:HMM:NF014679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR3343561.1"
                     /translation="MSKIAIVTDSCASIPESILETLNIHWVPYYIHRGKEVLRDLVTI
                     QRHTFYNWLPSAKQLPQTASPGPGDYLTMYQNLADQGTQEIISIHMTSKGSGAYQAAM
                     AAQSMVRELAPKLRVEVIDTLNVSMCHGWMVIESARAALSNKPFSDIVNLVKEMIPIT
                     RMIQTADTLKYLYMGGRIGKAKHLVGSMLNIKPLLSMENGIIVPVGTARSRKRAYQMM
                     VEKVEAAVGFMGKIKVAYVHAAAVEEAEKIRSLVEERLTCVESLIAELSPALGVHTGP
                     GTAGLCYFPVNT"
     gene            1840..3183
                     /locus_tag="AC16CB_13660"
     CDS             1840..3183
                     /locus_tag="AC16CB_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343562.1"
                     /translation="MYLEELVEVAVGLILVYILLSLAVSNIQEWISRWINNRSAFLET
                     TLREMLAETLNAPEGGRVTDRKSIPENKSGITEKVYNHPMIKSLYQGKGRPSYIPADK
                     FVLALFDIVVTAGSDGSTIEKALQKIKSYKERVPEASLAGLEAGIDELARQAKAARDD
                     PVKLAALQQDIENFAQEFKNASGFDVKPIFEGFLQAELPVKEEEVIQALKRGAAMLTI
                     ENLQLKQTLDDLVYQAEVYIKGAEGKLAQIRANAEKWFDDTMDRAAGWYKRSAQFWAF
                     YLGLILALFFNLDTVEIASRLWVQPTLRHSLIRAAETYQLEDINAQDEAPNPVETITR
                     LQSSLTGLHLPVGWAFQSLGPDLFNPAVDRCTLFPRSTSEVRAGKDVYGLSINGTCKR
                     WTNPPRGWGFLSKAIGLLITALAARLGAPFWFEILRKLVNVRSTGIKPEEKREKK"
     gene            complement(3246..4931)
                     /locus_tag="AC16CB_13665"
     CDS             complement(3246..4931)
                     /locus_tag="AC16CB_13665"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004467 - long-chain fatty acid-CoA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HIR3343563.1"
                     /translation="MDERPWLKRYDTDVPHQIDYPKVPLFALLEEAAQKYADAPCTIF
                     KGARITFREMNEITDRLAAGLAELGVKKGDRVGIFMPNTPQFVMAYFAILKVGGVVVA
                     TNPLYSGREIEHQVNDAGIEVMLVMSNFYNIIKQVQPKTKIRTVVVTNLKETLPPVLA
                     FLFTLTREKKGGFRVQLAEGDIWMQDLIARHKPEERPKVEVGPEDVALFQYSGGTTGI
                     SKGAIAPHRNLVANALQIRSWITNAEDGKETTLMAIPLFHVYGMVAGMLFSIRTGAAM
                     VMVPNARDLKDVLTNIQKYQTSIFPGVPTLYNAINNHPDVAAGKYNLSSVKACISGSA
                     PLMRETKEKFETLTGGKLFEGYGLSEAPTATHCNPLMGENRTGSIGLPLPDVECRIIS
                     LDDEVTVLPVGEIGELVVKGPQVFKGYHNMPTESANTLREGWLYTGDIARMDEDGYFY
                     IVDRKKELIKPGGFQVWPREVEEVISAHPKVMEVGVGGIPDPYRGETVKAWVVVNPGE
                     TLTEEEVRDWCKERLAKFKVPTHVEFRTELPKTTVGKILRRELVRQHKETAGE"
     gene            complement(5049..6155)
                     /locus_tag="AC16CB_13670"
     CDS             complement(5049..6155)
                     /locus_tag="AC16CB_13670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3343564.1"
                     /translation="MKPDYEIIVVGARIAGSIISTLLGEWGHRILVLDRASFPSDTLS
                     THFFRWPALTAFKRAGIFVEVQDAAPHLAVLYNDIDGHVISEPVQGQDNLDYFLCVRR
                     ITLDWILVQRMQQQPGIEFRQGARVKNLLWDADRVVGVKWEEDGQPFESKARLVVGAD
                     GKYSQVAREVQPVIEHSQPVQRAMYYTYFHGLDSLSGPAAEHYFRGNNLVYVFPTDND
                     LTLVAISVPIADFAVFRQDSEGQLMARLESLEALAPRLRRAERVAPVKGAGNIPCYQR
                     VPYGPGWVLVGDAAQILDPWSGQGIDHAGTHAVMLVEALHTFLSEEAPWDAAMAGYHS
                     SRNNWSRKTYQRTSSYAHDLRPMTRAALVKRGIA"
BASE COUNT         1570 a         1675 c         1521 g         1500 t
ORIGIN      
        1 atatccctga aaaccagtga tgacttgcac tcagggaatg ccatttctgc agacttcact
       61 gcccaaagct ggcatactca cctgaactta cccgatacac ctgtgatctc agcagaggaa
      121 gctggagcac gtctggctaa gttagcggtc gaccacgatt tttcaatgtt tgaattttgg
      181 ctgagcgatt atgctggtca tcatcaggac atgagcgctg catgctctct cttggaaacc
      241 ttcgaccagg ttataggcgg tcttctagac acctgggacg accattcagg cttgatctta
      301 atcacatctg accacggcaa tctggaagac ctatcgacgc gcaggcatac cttcaaccca
      361 gtgcctgccc tgctgatcgg cgcaagtgag ttgcgaagct cgttcgttca tctggcgaag
      421 gactcggcga aacgtcactc aagaggggga tcatcccatg gctctactcc gtacccagac
      481 aaaatttcgc ttaacctaac caacgtggct ccggcgattc tcggatttct gggtgagcat
      541 cactcgcagc gaagcgatct cgctataagc gattgagata gcttcgcctg cctacctggc
      601 ggcagcaggc aggtcagccc agaggccttc ccgccataac gatttaagat tgctcaggtt
      661 gcccacaagc ttccatcgca atgacggtag ggcaatcgca tttgggcgat ttcccaccac
      721 tgaggagaga aaatggggtg ttttggtggg ggcatagccc caccaaaaca cccctaacac
      781 tctttttgcg ggattgcgca tgcccctcag ggcatatgca attgctctgc aatgacggtt
      841 ttcgcgggaa agaaagcggg tatataatat tgattgagat ttcgtgacat caggaatatt
      901 acagcgacat tttatcatcc aagcaccgag gagatattta tgtctaagat cgccattgtc
      961 actgatagtt gcgccagcat acccgaatca atcttagaaa ccctgaatat ccactgggtt
     1021 ccttattaca tccatcgcgg gaaagaagtc ctccgcgatc tggtcaccat ccaacgccac
     1081 acattttaca attggcttcc ttcagccaag cagctacccc agaccgccag tcccggcccg
     1141 ggcgactacc tgaccatgta ccaaaatcta gctgatcagg gtacacagga aatcataagc
     1201 attcacatga catcgaaagg tagcggcgcc taccaggcag ctatggcagc ccaatcgatg
     1261 gtgcgcgaac ttgctcccaa gctgcgcgtt gaagtcatcg ataccctcaa cgtgtcgatg
     1321 tgtcatgggt ggatggtgat tgaatcagcc cgcgctgctt tgagtaataa gcccttttct
     1381 gatatcgtca acctggttaa agagatgatc cctatcacgc gcatgatcca aaccgctgac
     1441 acgcttaaat acctgtacat gggcggtcgg atcgggaagg ctaaacatct tgtagggagc
     1501 atgctgaata tcaagccctt gctcagcatg gaaaatggga ttattgtgcc ggtcgggaca
     1561 gcacgcagcc ggaagcgcgc ttaccaaatg atggtggaaa aagttgaggc ggcggttggc
     1621 tttatgggaa agatcaaggt agcctatgtg catgctgctg cggtcgaaga ggcagaaaag
     1681 atcaggtcgc tagttgaaga acgtttgact tgcgtagaat ccctaatcgc tgagctttcc
     1741 cccgccctgg gtgttcacac tggtcctggt acagcaggtc tatgttattt tcccgtcaac
     1801 acatagttaa atacttccga agttacaaaa ggaggagcaa tgtatttgga agagctggtt
     1861 gaagtcgccg taggtctcat cctggtatac atcttgttga gcctcgcagt ttcaaacatc
     1921 caggagtgga tctctcggtg gattaataat cgctctgcat ttctcgagac taccctacga
     1981 gagatgctag cggaaacctt aaacgcgccc gaaggtggtc gcgtcacgga tagaaaatcc
     2041 atcccagaaa ataaatcagg gataacagag aaggtgtaca accacccaat gatcaaatcc
     2101 ctatatcaag gtaaagggcg accctcctac atccctgccg acaaattcgt gttagccttg
     2161 tttgatatcg ttgtgactgc cggcagcgac ggctccacga ttgagaaagc actgcaaaag
     2221 attaagagct acaaagagag agtccctgaa gcatcgctgg ctggtttgga ggctggcata
     2281 gatgagttgg ccaggcaagc caaggctgct cgagacgatc cagtgaaact agctgctttg
     2341 caacaggaca ttgaaaattt cgcccaagag tttaaaaatg ccagtgggtt tgatgtaaaa
     2401 ccaatttttg agggcttcct gcaagccgag ctgccagtaa aagaggagga ggtgatccaa
     2461 gctctgaagc gtggcgcggc tatgttaaca atagaaaatc tacagctaaa gcaaacccta
     2521 gacgacctgg tttaccaagc tgaggtatac attaaaggag cagagggtaa actagcccag
     2581 atccgcgcca atgcagaaaa gtggtttgat gacacgatgg accgcgcggc tggttggtat
     2641 aagcgcagcg ctcaattttg ggctttttac ctggggctaa ttttagcgct tttcttcaac
     2701 ctggataccg tcgaaatcgc ctcccgcctt tgggttcaac ccacactacg ccacagcctg
     2761 attcgagctg ctgaaaccta tcaattagaa gatatcaatg cccaagatga ggcgcccaat
     2821 ccagtagaga caatcacccg cttgcagagt tcattgacag gcttgcactt gcctgtggga
     2881 tgggccttcc agtccctagg acccgattta ttcaacccgg ctgttgaccg gtgtactctg
     2941 ttcccccgct cgacctccga ggttcgtgct ggtaaagacg tatacggtct atcgattaat
     3001 ggcacctgca aacgctggac caacccacca aggggttggg ggtttctaag caaagctatc
     3061 ggcttattaa tcaccgccct agcagcccgg ctgggcgcgc ctttctggtt cgaaatcctc
     3121 cgaaagctgg tcaacgtgcg ctcaacgggt ataaaacccg aagagaaaag agagaagaaa
     3181 taaaaacggg ttatcggacg gcgtaactgt cgcccaataa cccgcgttgt ttcgctaatt
     3241 aaagtttatt ctcccgctgt ttctttgtgc tggcgcacca actcacgccg caggattttg
     3301 cccacagtcg tctttggcaa ttcagtgcga aattccacat gcgtcggcac tttgaatttt
     3361 gccagccgtt ctttgcacca atctctgact tcctcttcgg tgagagtctc gcccgggttg
     3421 accactaccc atgcttttac ggtttcgccg cggtatgggt cagggatgcc tcccaccccc
     3481 acttccatca ctttcgggtg agctgaaatc acttcctcca cctcacgggg ccaaacctgg
     3541 aaacctccgg gcttgatcag ctctttctta cgatcaacga tgtaaaaata tccgtcctcg
     3601 tccatacgag cgatatcgcc agtatacagc cacccttcac gaagcgtatt tgcggattcc
     3661 gtgggcatat tatgataccc tttgaaaacc tgcggacctt taacgaccag ctcaccgatc
     3721 tctccgaccg gcaaaacggt gacctcatcg tcaaggctga taatacggca ttccacatca
     3781 ggcaagggta aaccaatcga gccagtgcgg ttttcaccca tcaatggatt gcaatgtgtg
     3841 gcagtgggag cctctgaaag tccatacccc tcaaagagtt taccgccggt aagcgtttcg
     3901 aatttctctt tcgtctcgcg catgagtgga gctgagccag agatacatgc tttcaccgaa
     3961 ctcaagttat acttccccgc tgccacgtcc gggtgattat tgatggcgtt atacaatgtg
     4021 ggcaccccgg ggaatataga ggtttgatac ttctggatat tggtgaggac atccttcaaa
     4081 tcacgcgcat tggggaccat gaccatcgca gcgcccgttc ggatggagaa aagcatacca
     4141 gccaccatcc catacacatg gaagagcggt atagccataa gagtcgtctc ttttccatcc
     4201 tcggcatttg ttatccagga gcggatctgg agtgcattgg cgactaaatt tcggtgtggg
     4261 gcaatggcgc ctttagaaat tcctgtggta ccgccgctgt actgaaaaag tgccacatcc
     4321 tcaggaccaa cttctacttt cgggcgctcc tcgggtttgt gccgggcaat caaatcctgc
     4381 atccaaatat cgccttcagc caactgcacg cggaatccac ctttcttctc gcgtgtcagc
     4441 gtgaacagaa aagccagaac tgggggcagg gtttccttca aattggtcac cacgacagtt
     4501 cgtatcttcg tctttggctg tacctgcttg atgatattgt aaaaattgct catcacaagc
     4561 atcacttcaa tgcccgcgtc gttgacttgg tgctcaatct cgcgcccact gtacaatgga
     4621 ttggttgcca cgaccacccc acccaccttg aggatagcaa aatatgccat cacaaattga
     4681 ggcgtgttag gcatgaagat gcccactcga tcgccttttt tcactccaag ttctgctaaa
     4741 cccgccgcta gccgatcggt gatctcattc atttcgcgga atgtgattct ggctcccttg
     4801 aaaatcgtac agggagcatc agcatacttc tgagctgctt cctcaagcaa tgcaaacagg
     4861 ggaaccttag ggtagtcgat ttgatgcggc acatccgtat cgtatcgctt aagccacggt
     4921 cgttcgtcca tatcctcctc caaatgttgt gagacaaacc gatttaggat aaatgatcac
     4981 agatagtata gagtgaacca tctggaaagt caattggttt ttggaacccc ccatcaaggc
     5041 aggtcctgtc aggcgattcc gcgcttcacc agggctgccc ttgtcatggg ccttaggtcg
     5101 tgagcgtaag agctggtgcg ttggtaggtc ttacgactcc aattattacg agacgaatgg
     5161 tatccagcca ttgccgcatc ccaaggtgct tcttcgctca ggaatgtgtg gagagcctct
     5221 acaagcatca ccgcatgcgt tcctgcatgg tcaattccct gacccgacca gggatccagt
     5281 atctgagccg catctccgac caacacccat cccggaccat aaggcacgcg ttgatagcat
     5341 gggatattcc cagcgccttt aactggcgca acccgctcgg cacggcgcaa tcggggggct
     5401 agagcttcca atgactccaa tcgcgccatg agttgccctt ccgaatcctg gcgaaagact
     5461 gcaaaatcgg cgatcggtac tgagatagct acaagggtta ggtcattatc cgttggaaat
     5521 acatagacca aattgttccc acgaaagtaa tgctcagccg cagggccaga taaagaatcc
     5581 agaccgtgga aatatgtata atacatggct ctttgcacag gctggctgtg ctcaattacc
     5641 ggctgcacct ctcgcgccac ctgagaatat ttcccatctg ctccaacgac aagtcgggct
     5701 ttggactcaa atggctgccc atcctcttcc catttgactc ccaccacgcg atccgcatcc
     5761 caaagcagat tttttacccg ggcaccctgg cgaaactcaa ttccaggttg ctgttgcatc
     5821 cgttgaacaa gtatccaatc tagagtaatg cggcgaacgc acaagaaata atccaagtta
     5881 tcctggccct ggacaggttc gctaatcaca tgcccatcga tgtcattata gagcactgcc
     5941 agatgtgggg ccgcatcctg cacctctacg aatataccag cccgcttaaa ggctgtcaga
     6001 gctggccagc gaaagaaatg ggtcgaaagc gtatcacttg ggaagctcgc gcgatccaag
     6061 acgagtatcc gatgtcccca ctcgccaagc aaggtgctta tgatcgagcc agctatcctg
     6121 gctccaacga caatgatctc ataatcaggt ttcataacat cttctggtgc agtctatgca
     6181 caaagttgat caatgcctgg cacgaccaag agccttgagc gtatcttgca tcctagtggt
     6241 cgtcaagagt ctcgggggta tatttt
//
LOCUS       DBNGSX010000461         2868 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1846042, whole genome shotgun sequence.
ACCESSION   DBNGSX010000461 DBNGSX010000000
VERSION     DBNGSX010000461.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1846042"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1580)
                     /locus_tag="AC16CB_13675"
     CDS             complement(66..1580)
                     /locus_tag="AC16CB_13675"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3343565.1"
                     /translation="MRHFNIAFENENSRSRNRQGTPVLPIIAKFYNQNRHATMVFPLE
                     GSYIDEVLYIIRIIKQIEKMILSSEPQQSYSSASQPIAAQNSAIAYGLGSFGLESLYK
                     VFVGFYMFYYIDQLGLAVALAAVINVIFAIWDSVNDPLAGFLSDNTRTRWGRRKPWLL
                     VGLPFYVGFLVMVYAVPEPFLQGKALFWYALGIFILFEAAYTMMAINYAALFPELFQG
                     IRERARASSTYQGLGMLGELVGFSIPPFIYAEFGFVPMAISFAGVAGITLFMGIIRNR
                     EDPRALKAPPLDLKGAFGEVLKDRSFLLFTIAFTFLMFITAAYTLATPFWVKYTLGAS
                     PQVTSLIFAIVFIVAILSVSIWGRLVRILGIKRSWLWSVGVMAASAIIVGLATNLIAG
                     AIGAAVAGAGLGGVKVCREMIMANFVDQNLKRTGHRREGMYYSLLRVMGKLSKILESL
                     VLVLLSLLFGYVSGENPGPQPGNAFRFLISVFPLVFTIIAWLISSRLTFENEQA"
     gene            complement(2021..2176)
                     /locus_tag="AC16CB_13680"
     CDS             complement(2021..2176)
                     /locus_tag="AC16CB_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343566.1"
                     /translation="MATVYAVMEKYNRLIIFSLLGVIVLFLFACTFHDILPICHYLFG
                     CDHMMHL"
     gene            complement(2239..2607)
                     /locus_tag="AC16CB_13685"
     CDS             complement(2239..2607)
                     /locus_tag="AC16CB_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343567.1"
                     /translation="MTIEQNTSMKKERQRLEGLWWAAALIWAGLVLGADSMGFLPQIG
                     SADTWSWIFLGAGVFGTLGNLYRMGSSDAPNPTSWDWIWSGAFLILGLGGFAALNISW
                     PLILILFGGIILVNVLWQRD"
BASE COUNT          780 a          712 c          649 g          727 t
ORIGIN      
        1 cccccccaaa ctccccctct ttccccttca actgcgtaag tcctatagat tatattccgg
       61 tttggctatg cctgctcatt ttcaaacgtg agcctgctgg aaatgagcca ggcgatgatg
      121 gtgaacacaa gcggaaacac gctgatcaaa aagcggaagg catttcctgg ttgtgggcca
      181 ggattctcgc cgctgacata accaaaaagc aggcttaata gcactaaaac gagcgactcc
      241 aggatctttg agagctttcc catgactcgc agtaggctgt agtacattcc ctctcgacgg
      301 tgaccggtgc gtttcaggtt ttgatccaca aaattggcca tgatcatttc gcggcagact
      361 ttgacccctc ccaggcccgc cccggctact gcagcgccaa tagctcctgc aatcaggttg
      421 gttgctaatc ccacaataat cgctgatgcc gccatcacac ccacagacca cagccaggat
      481 cgcttgattc ctaaaatacg tacaagtctg ccccaaatgg agactgacaa aatagctacg
      541 ataaacacaa ttgcgaaaat gagtgaagtt acctgcgggc tggctccaag cgtgtactta
      601 acccaaaatg gggtcgccag ggtatacgct gcagttataa acatgagaaa ggtaaatgca
      661 atggtgaaca acaaaaagga gcggtcttta agcacctccc cgaaagcgcc tttcagatcc
      721 aatggcggcg ccttcaaggc tcttggatct tctctatttc ggataatccc catgaagagc
      781 gtgataccag ccaccccggc aaagctaatt gccattggaa caaaaccgaa ctcggcataa
      841 ataaaaggtg gtatagagaa cccaaccagc tcgccaagca taccaagacc ctggtaggtt
      901 gaacttgccc gcgctcgctc tcggataccc tggaagagct caggaaatag cgctgcgtag
      961 ttaatcgcca tcatcgtata ggctgcttca aataggataa atatccccaa cgcataccag
     1021 aataacgcct ttccttggag gaaaggttca ggcaccgcgt aaaccattac caagaaaccc
     1081 acgtagaagg gcagtccgac caagagccag ggcttccgcc ttccccagcg agtgcgcgta
     1141 ttatccgaga ggaatcccgc cagtggatcg tttaccgagt cccagatcgc aaagatgaca
     1201 ttgatgaccg ctgccagcgc gacggcaagc cctaactggt cgatgtagta aaacatataa
     1261 aaacccacaa acactttgta gagggattcc aacccaaatg accccaatcc atatgcaatg
     1321 gcggaatttt gggccgcaat tggctgggat gcgctcgaat agctttgttg gggttcagat
     1381 gaaagaatca ttttctctat ttgcttgatt atcctaatta tatacaaaac ctcatcgata
     1441 tatgaccctt ctaaaggaaa caccatcgtt gcatgacggt tttgattgta gaattttgct
     1501 atgataggca acactggcgt tccttgacga ttccttgatc gagaattttc attctcaaaa
     1561 gcaatgttga aatgacgcat ttccccataa gcggtgttcg gctcaggttc tcgtagggac
     1621 aaatgattca gaaaaccgtt ctacggatga tgtggagtgg tttttgtcat ctattaagtt
     1681 tgaaaatcgt gaataattgg gtgaaataag tctggactat ggattctgat cgcatcttgg
     1741 agttcgaatc ctcttcgttg gtagtcatcc cattattggc tggagtcctt ttaggataaa
     1801 cgtcagatca acaaagtaga tattttggta tttcttcaag aaagactcta tgtcatgtgg
     1861 ctttgtaaac cagttgagtg ttttgtgtag aatattgtaa taggcaatcg tctctacaac
     1921 cagatcaggg cccagctcat tttctgcatt atgcgcataa ttggattggg cccttaaacc
     1981 tggctgcggt tggggagcat caagctgatc gagaaggggg ctatagatgc atcatatgat
     2041 cgcacccaaa cagatagtga catatgggta gaatgtcgtg gaaagtgcag gcgaagagga
     2101 acagaacgat cacacccaag agcgaaaaga tgatgagccg gttgtatttt tccattactg
     2161 catatactgt tgccatttca gacctccttt gattatgtgg tttgatctca aaccgtcttc
     2221 ttgaaaattg atagggtcct aatcacgctg ccagagtacg ttgaccagga taatcccgcc
     2281 aaacaggatc aggatcaacg gccaggaaat gttcagcgcg gcaaacccgc ccaggccgag
     2341 gatcaggaag gcgccactcc aaatccagtc ccaagaggtt gggttcggag catcagatga
     2401 gcccatccgg taaagattac caagagtgcc aaacactccc gctccaagga agatccagct
     2461 ccaggtatcc gcgctaccaa tttgaggcag gaaccccatg ctgtcagcgc caaggaccaa
     2521 cccggcccag atcagagctg ctgcccacca cagcccttcc agacgttgtc gttctttttt
     2581 catacttgtg ttctgttcaa ttgtcatttt tctctccttt ctatttcagt ttcgattggc
     2641 ttgttataac tatacaaatt aatgtggtga tgtgtcatca catcatttgg tgattggtat
     2701 ggtgattaag tgtgattatt gcattcattt cactctacct ttctttcgtt tatattcaag
     2761 atgccccctc acttttcccg aaaagcgttt cctttttaag gctaagtgtc agttcacatg
     2821 ggcacatcca atcaccgacc agccacgctc tgctgccagg gcagccag
//
LOCUS       DBNGSX010000462         4539 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1569667, whole genome shotgun sequence.
ACCESSION   DBNGSX010000462 DBNGSX010000000
VERSION     DBNGSX010000462.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1569667"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2069)
                     /locus_tag="AC16CB_13690"
     CDS             complement(<1..2069)
                     /locus_tag="AC16CB_13690"
                     /inference="COORDINATES: protein motif:HMM:NF014900.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HIR3343568.1"
                     /translation="MNRRIEELKRRVRDGERQVLRKEEPIDVLTECDVEKLSWMKRAA
                     RLTVRQCEAEMVVIEPDERIVFTRTLPAQIPTIYSREDWERLTAGRTIHELGPINNVT
                     ADWGKALAMGLLGRKKVALASRERYRHNLESVEFLDSAIETIDAVLALASRYAEAARQ
                     MNHDEVADILDHVPANPPRTFHEALQSLRLMQAVAWLTGHYHVGFGRFDQYMWPYLKS
                     DLDSGRIDKNQAAELLAEFFISLNKDSDLYPGVQQGDNGQTITLGGVDREGKEAINEL
                     TYLVLRVSRDLGLIDPKINLRISASTNLDLLSLATELTRKGLGFPQYSNDDVVIPALV
                     KHGYSLQDARDYSVAACWEFIIQGKGMDVVNIGAVSFPAAVDKAIRQGFLAGEDMDGI
                     LRRVDADIKQQVNRLASSYERLLLPPAPYYSMLMTDCLERGQDLSKGARYNNFGIHGA
                     GSANAADALAAVQKFVFDDGCVEPSELLSALDANYEGYEKLCSRLAEDGPKVGNDDDR
                     ADLFMMRLFNMLADACENYGRTSRGGILRPGSGSAMYYIWLSQGFEGMREPLVGATAE
                     GRQKGIPFGANLAPSQGIQIRGPFSVLLSYAKLDYQRVCNGGPITMELSDSVFRNDES
                     IRKVALLVRSFAQLGCQQLQLNSLNVDTLRDAKIHPQLHKNLIVRVWGWSGYFCELSP
                     EYQDQIIS"
     gene            complement(2066..2821)
                     /locus_tag="AC16CB_13695"
     CDS             complement(2066..2821)
                     /locus_tag="AC16CB_13695"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3343569.1"
                     /translation="MITGIVFDIKEFAVHDGPGLRTTVFLKGCPLSCIWCHNPEGQSS
                     TPQEMRSGAGVRHVGREYSSQELAALLNRQATILKTGEGGVTFSGGEPLMQADFVAEA
                     IDLLEDFHILLDTSGYGKERDFRLLLERVNLVYYDLKLIERQAHKRYTGCDNDLILQN
                     LHTLNTSGVPFVIRVPLVPSVTDTDENLAAIAGVIKGMPGMLGVDLLPYNRAAGGKYR
                     AAGMEFQPQYDETSLVNANVAIFEKAGIKARIV"
     gene            complement(3073..3858)
                     /locus_tag="AC16CB_13700"
     CDS             complement(3073..3858)
                     /locus_tag="AC16CB_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012473586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3343570.1"
                     /translation="MIYDFDGKKYEKASIHQQEWGNKLISELNLFGNEKILDLGCGNG
                     TLTANLADLVPNGSVLGIDASQGMLDVARYKKRDNLTFKLMDINKLELSGKYDVIFSN
                     ATLHWIKDHQSLWKNVKNLLSEQGIVRFNFAADGNCQYFFRTIHKAMMLDEYKKFFKH
                     FEWPWYMPSIEEYKKLVKPFSLSEIKIWGEKADYLFPDSESIVGWINQPSIVPFLKLL
                     PENQKELFRNFVIEQMLKNTAQENGGYFETFRRINIYAKNANA"
BASE COUNT         1191 a         1160 c          941 g         1247 t
ORIGIN      
        1 gagataatct ggtcctggta ctctggactg agctcacaaa aatagccgct ccaaccccat
       61 acgcgtacaa tcaaattctt atgtaattgc ggatgtattt ttgcgtctcg aagtgtatcg
      121 acgttcaggg agttcaattg tagttgttga caacccagtt gggcaaaact ccgtaccagc
      181 agagcaactt tgcggatcga ttcatcgttg cgaaagacag aatccgacaa ctccattgtg
      241 attgggccac cgttacacac ccgttggtaa tccagtttgg cataggataa aagcacgcta
      301 aagggaccac ggatttgtat tccctgggag ggagccagat ttgccccaaa tgggattccc
      361 ttctggcgtc cttctgctgt tgcgcccacc aaaggctcac gcatgccctc aaagccttgc
      421 gacagccata tataatacat agccgagcca gatccaggtc gtaaaatccc tcctcgcgag
      481 gtccggccat aattttcgca ggcatcagcc agcatattga acagtcgcat catgaacagg
      541 tctgcccgat catcatcatt acctaccttg gggccatcct cagctaacct tgagcataac
      601 ttctcatagc cctcataatt ggcatccagc gcggagagta actcagatgg ttctacacaa
      661 ccatcatcga atacaaactt ctgcaccgct gccaacgcat cagccgcatt tgccgatcca
      721 gcaccgtgaa tcccgaagtt gttgtacctg gctcctttcg acaagtcttg tccgcgttcc
      781 aggcagtcag tcatgagcat tgagtaataa ggggctggag ggagcaataa gcgctcataa
      841 gaggacgcta accgattcac ctgttgtttg atatctgcat ctaccctacg aagtatccca
      901 tccatatcct ccccagccag gaagccctgg cggatggctt tgtctacagc tgcagggaag
      961 gagactgcac ctatgttgac cacatccatg ccttttccct ggataataaa ttcccagcat
     1021 gctgcgacag agtaatcgcg agcatcttgc aggctataac catgtttcac cagcgcggga
     1081 ataaccacgt cgtcgtttga atactgcgga aaacccaatc ccttgcgagt caattcggtc
     1141 gccaacgaaa gcaaatccag atttgtgctc gccgatatac gcaagtttat cttggggtca
     1201 atcaagccca aatcgcgcga taccctcaac acaagatagg tcagctcgtt gattgcttct
     1261 ttaccctctc tatccacacc accaagggta atggtctgtc cattatcgcc ttgctgtaca
     1321 ccaggataaa gatcgctatc tttatttaac gagatgaaaa actctgctag aagttctgct
     1381 gcttgatttt tatctatgcg tccgctatca aggtcagatt ttaagtaggg ccacatatat
     1441 tgatcgaaac gcccgaagcc cacatggtag tgaccggtca gccaagctac agcttgcatc
     1501 agacgaaggg attgcagcgc ttcatgaaag gtacgcggcg gatttgccgg gacatgatcc
     1561 aagatatcgg caacttcatc atgattcatc tgacgagccg cctcggcata gcgcgacgcc
     1621 agagccaaaa cggcgtcaat ggtttcaatt gcactgtcca ggaattcgac tgattccaga
     1681 ttatgcctat accgctcccg tgaagccaac gcgacttttt tgcgccctag cagccccatc
     1741 gcgagcgcct taccccaatc ggcagtgaca ttgttgatcg ggccaagctc gtgaatcgtt
     1801 cttcctgcag tcaagcgctc ccagtcttcc cttgaataaa tggtcgggat ttgtgcaggc
     1861 agtgttcggg taaatacaat ccgctcgtcc ggctcgatca caaccatctc cgcttcacac
     1921 tgccgtaccg tcaggcgggc cgctcgcttc atccatgata gtttctcaac atcgcattcg
     1981 gttaaaacgt cgatgggctc ctcctttcgc agcacctgac gctcgccatc gcgcacccta
     2041 cgctttagtt cttctattct tctattcata cgatacgcgc ctttattccc gctttttcaa
     2101 atatagccac attggcgtta accaggctgg tttcatcgta ttgcggctga aactccatgc
     2161 cagccgcccg gtacttccca cccgcagccc ggttatacgg cagcaaatcc acgccaagca
     2221 tacctggcat accttttatc accccggcaa tcgcagctag attttcgtct gtatcggtaa
     2281 cacttggtac aagcggcaca cggatcacga atggaacgcc actggtattc aaggtgtgca
     2341 agttttgcag aataaggtcg ttgtcacatc cagtatatcg cttgtgtgcc tgacgctcga
     2401 tcagcttcag atcataatat acaagattca cccgctccag gagtagacga aagtctcttt
     2461 ctttaccata gcctgaggta tctaacaaga tgtggaaatc ttccagtaaa tcgatggcct
     2521 ccgcaacaaa atctgcctgc attaaaggct ctccccccga gaaggtgaca cctccctccc
     2581 ctgttttaag tatggtcgct tgccgattca gtaaggcagc caattcttgc gacgaatatt
     2641 ctctacccac atggcgaaca ccggccccgc tccgcatttc ctggggtgta ctcgactgtc
     2701 cctcaggatt atgacaccaa atacaagaaa gagggcaccc ctttagaaag accgtggtgc
     2761 gcaatccagg tccatcgtgt acagcaaact ccttgatatc gaagacaatt cctgtgatca
     2821 tttacaatct caagtatttt gaaaaacgtg ttacagtgga acccgtctcg tcatatttta
     2881 tcccgaatca attcctgccc gctagtcaca gggatatata tgtgagtggc gcggcccatt
     2941 agtgaacttg gagcttcaca gttgcataaa tttttcccac ttttcgggtg ccaccctacg
     3001 cgtctccccg tgcaattggg gagcaggaag catggcagca atcaccgcct gcctcaaaag
     3061 ttcagcaaat ttttatgcat ttgcgttttt cgcgtaaata ttaatgcgtc taaatgtttc
     3121 gaagtatcca ccattctctt gggctgtgtt ttttagcatc tgctcaatca caaaatttcg
     3181 aaacagctct ttttgatttt caggcaataa ctttaagaat ggaacaatgc tcggttgatt
     3241 tatccatcca acaatcgatt cgctatcagg aaataaataa tcagccttct caccccatat
     3301 ctttatctct gaaagggaga aaggtttaac aagttttttg tattcttcga tgcttggcat
     3361 gtaccagggc cattcaaaat gcttgaaaaa ttttttatat tcatctagca tcattgcttt
     3421 atgtatggtc ctgaaaaaat attgacaatt gccgtccgcc gcaaagttaa agcgcacaat
     3481 gccttgttca gacaataagt ttttaacatt tttccataaa ctttgatgat cttttatcca
     3541 gtgcagggtc gcatttgaga aaataacatc atattttccc gataactcca gtttgtttat
     3601 atccattaat ttaaacgtta ggttatccct ttttttgtat cgcgccacat ctaacatacc
     3661 ttgagaggcg tcaataccta aaacgcttcc gtttggcact aaatctgcaa gatttgccgt
     3721 aagagttcca ttgccacatc caagatcaag tatcttttcg tttccaaaaa gatttagttc
     3781 ggaaatcaat ttattgcccc attcttgttg gtgaattgag gctttctcat attttttgcc
     3841 atcaaaatca tagatcataa tgtgttatgc ctaatacttt gatctcggac ttgttctaga
     3901 ttgcaaattt gctgataatg cggtcttaca agaggggtac gctgcgttat gtgtgtaccc
     3961 aatacagacg cctttgcatc gttgttgtaa ctcctagcta actccagtat atagattaaa
     4021 ctctcaaggt gcaacatcaa ccctccaaaa aatcctaaca cgatccattt acaggcgatt
     4081 atagttaacg gtaccgatcc aaaagtgaac attgagggtg tatcacaatt aaaaaattcc
     4141 tttttactag tagcagtagt ggtccatcat atcacctgga cactagtgta gcttggcgcg
     4201 tgtgtatcat aagagtgagc atcttatgct attatgatta ggataaagat aaccgcctcc
     4261 aagtgtgtta ggggcggctg tttagagggc taatatttca actgggctta gcttctttta
     4321 tacatacctc aagtagtgcg attattacaa accattaggc ttgcagtggc aagtgcctag
     4381 gctcgcatag actgtaaaga tatgatctga agcgtaaata tgtcaatgtt ttgctccaaa
     4441 gaagtattga tccagacaaa ttgagatagt gggatcatgg atcagcctcg tgcccactta
     4501 atgaaaattg aggtggcttg ccacccaaaa gcacgacca
//
LOCUS       DBNGSX010000463         2188 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_392024, whole genome shotgun sequence.
ACCESSION   DBNGSX010000463 DBNGSX010000000
VERSION     DBNGSX010000463.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_392024"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            336..>2188
                     /locus_tag="AC16CB_13705"
     CDS             336..>2188
                     /locus_tag="AC16CB_13705"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HIR3343571.1"
                     /translation="MTKITREDALEYHRLGGKPGKVSVVPTKPMATQRDLSLAYTPGV
                     AVPVLEIEKDPELGYEYTSKGNLVAVVSNGTAILGLGDRGALASKPVMEGKGVLFKRF
                     ADVDVFDIEVDTHDPDEVIQVVAAISPTFGGINLEDIKAPECFYIEEKLKEMLNIPVF
                     HDDQHGTAIISGAALMNGLEIVGKRIEEARITISGAGASAISCAELMVRLGARRDNIL
                     LVDTRGVVYQGRTEGMNKYKERFAHDTDARTLADAMRGADVFYGLSIANILTPEMVKT
                     MAESPLIFAMANPDPEIRYELAKEARPDAIVATGRSDYPNQINNVLGFPFIFRGALDV
                     RAKAINEEMKVAASHALSALTKEDVPDSVLRAYGLESLKFGPDYIIPKPLDPRVLLWE
                     SPAVAKAAMDSGAARRHIDLEEYGEQLAYRQGMGEQVRYYIMHQAQKCPERKRVVFAE
                     GEESRIIRAAAQIVEEGIATPILIGRSDVVTQKIVALGLDCCPEIIDPNSFERIGEYA
                     QAYYEIRQRKGVVLQDAKKRVCEPNVFGPMMVAMGDADAFVSGLTYDYPEVLRPALQI
                     HHTHPGAERAAGVYIMIVDSRTYLFTDATVIIDPTAEDLAEIACLAADYAR"
BASE COUNT          532 a          510 c          596 g          550 t
ORIGIN      
        1 cttttccttc atctgcgcag gtcatattct tcttgttttt acatcatgcc aagtaaacca
       61 gatataatat gtcatattag ttgcggatga cctagtaaga ttgcgttatc aatttgatag
      121 tttcgcctgt caggagttta acttcgattg aggtgctgct ggatcaccaa tatctttttt
      181 ggtaagccat gtaattcatt taggcaagtt atccaggaaa taatgtgaca aaagctacta
      241 tgcgggtgaa gaaaattatg cgaatattat tgcattgact ggagaccatt cttcgatacc
      301 tgaaaacccc aataccaatc gtgaaggagt tgaatatgac caaaataacc cgtgaggatg
      361 ctctcgaata tcatcgcctg ggtgggaaac ctggtaaagt ttcggtggtt ccaaccaaac
      421 cgatggcgac ccagagagat ttgagtctgg catacacccc aggcgttgct gttccggtct
      481 tggaaattga gaaagaccct gagttaggct atgaatatac ttccaaggga aacctggttg
      541 cagttgtttc taacgggaca gccatactcg gtttgggaga ccggggggca ctggcgtcaa
      601 aaccggttat ggaaggcaag ggcgttctct ttaagcgctt cgctgatgtg gatgttttcg
      661 atatcgaggt tgacacccat gatcctgacg aggtgattca ggttgttgca gccatttctc
      721 cgacatttgg cggcatcaat ttagaggata ttaaagcccc agaatgcttc tacatcgaag
      781 agaagctgaa agaaatgctg aatatcccag tctttcatga tgaccagcac gggacggcaa
      841 tcatctctgg cgcagcttta atgaatggct tggaaattgt gggtaaacgc atcgaggagg
      901 cccgaattac catatcaggg gcgggagctt cggctatttc gtgcgctgaa ctgatggtaa
      961 ggcttggggc acgccgcgat aatattcttc tcgtcgatac caggggagtt gtctaccagg
     1021 gccgcacgga aggtatgaac aagtataagg agcgttttgc tcatgatacg gatgcccgca
     1081 cgctagccga tgccatgcgt ggcgctgatg ttttttatgg cctctccatt gcgaatattc
     1141 tgacacccga aatggtgaaa acgatggcgg aaagtccctt gatctttgcc atggctaatc
     1201 cagatccgga gattcgctat gaactggcga aggaagcccg ccctgatgcg attgtcgcta
     1261 caggtcgctc ggattaccca aatcagatta acaatgtgct tggtttcccg ttcatattcc
     1321 gtggcgctct ggatgtgcgt gccaaagcga tcaatgaaga aatgaaagtc gctgcctcgc
     1381 atgctttgtc agccttgacc aaggaggacg ttccggatag tgtgctgcgc gcatacgggc
     1441 tggaaagcct gaagttcggc ccggattata ttatccccaa gccgctagac ccgcgcgtcc
     1501 tgctgtggga atcgcctgca gtcgccaagg cagccatgga ctccggtgca gctcgccgcc
     1561 acatcgattt ggaagagtat ggcgagcaac tagcctatcg ccaggggatg ggggaacagg
     1621 tgcgttatta catcatgcac caggctcaga agtgcccaga gcgcaagcgt gtggtctttg
     1681 cggaaggcga agagtctagg atcatccggg ccgcagccca gattgtagag gaaggtatcg
     1741 cgactccgat cctgataggt cgatcagatg tagtcacgca aaaaattgtt gccttaggtc
     1801 ttgactgctg ccctgagatt atcgatccaa atagtttcga gcggattggg gagtatgcac
     1861 aggcttatta cgaaatacgg caacgcaagg gggttgtcct ccaggatgcc aagaagcgtg
     1921 tgtgcgagcc gaatgttttt ggcccgatga tggtcgccat gggtgacgca gacgcctttg
     1981 tctctggtct aacctacgat tatcctgaag tgctccgtcc tgctttgcaa atccaccaca
     2041 cccatccggg tgcggagcgg gcagcaggtg tgtatattat gatcgtcgat agccggacat
     2101 acctgtttac cgatgcaacc gtgatcatcg atcccaccgc tgaggatctg gcggagattg
     2161 cttgcctggc agccgattac gcccgcca
//
LOCUS       DBNGSX010000464         2037 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1361280, whole genome shotgun sequence.
ACCESSION   DBNGSX010000464 DBNGSX010000000
VERSION     DBNGSX010000464.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1361280"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="AC16CB_13710"
     CDS             <1..312
                     /locus_tag="AC16CB_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HIR3343572.1"
                     /translation="TIIEKDLAPGDVLRVDTGCLVAFATTVDYDIQFIGGFKNVLFGG
                     EGLFLAKLTGPGKVYLQSLPLSRLADRINAAARGRQVDERKGVAGMGGDILGNILSGN
                     S"
     gene            637..1629
                     /locus_tag="AC16CB_13715"
     CDS             637..1629
                     /locus_tag="AC16CB_13715"
                     /inference="COORDINATES: protein motif:HMM:NF024930.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0005085 - guanyl-nucleotide exchange
                     factor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RCC1 domain-containing protein"
                     /protein_id="HIR3343573.1"
                     /translation="MSNRFYRFLCFTICVGLITHPGQAASAPKASNASDIAWEAGLDK
                     MSSVSAGLDHTCGIKLDGTVACWGSNSDGKTDPPAEVFSLISPGDDHKISAGTFHNCG
                     IKQDGTVECWGNDDDGQSSPPVGTFKQISAGGSHTCGVKADDTVACWGLDLDGQTSPP
                     QGEHFKQVSAGIEHTCGVKLDETLACWGNPFLPEIYDTPDGTFKQISAGGYHNCAVKK
                     SDNTLACWGEGTENTGIDPDYGQAMPPGDAFTQVSAGGYHTCGIRTNGTVACWGYNYY
                     NQATPPAGVFTQVSAGDFHTCGIRQDGTVTCWGDNSSGQAPRGVTPLFLPLVSR"
     gene            complement(1634..>2037)
                     /locus_tag="AC16CB_13720"
     CDS             complement(1634..>2037)
                     /locus_tag="AC16CB_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HIR3343574.1"
                     /translation="RNANGKAEAGLLDGVAQALPALVQAQEYQKRAGRVGFDWPNIQG
                     VIDKVHEELREVNAARTANDRAAEIGDLLFAVVNLARWVDVDAESALREANARFRSRF
                     AQIEVATRAQNRSITDLSLDEMEALWQAAKK"
BASE COUNT          458 a          576 c          572 g          431 t
ORIGIN      
        1 acgattatcg agaaagacct ggctcccggc gatgtgctgc gcgtcgatac tggctgcctg
       61 gtggcttttg ccaccacggt ggattacgat atccagttca tcgggggttt taagaacgtg
      121 cttttcggcg gtgaagggct gtttctagcc aagctgaccg gccccggcaa ggtctacctg
      181 caaagcctgc cgctatcccg cctggccgac cgcatcaatg cagctgctcg cggccgtcag
      241 gttgatgagc gcaagggtgt tgcgggaatg ggcggagaca tcctgggcaa tatcctcagc
      301 gggaactcgt agaatcgcat tattcgtttc gaagcccatc catagggtaa ttgcatcgta
      361 tccaatcacc cccaggctcc gtcatgcagc ctgggggcga ttggcttcgt tttaggcaat
      421 gccaaccaaa aattgcttga cgagatcaat ttattgggta tactgagggg cagttattaa
      481 gcatagctgc gcatctatcg taagcgcgta tttcgctcag aggggaagaa cgcagggagc
      541 tggcaagaaa ccacacgctt cccatgccat gtcggcggca tgacatcccc atctagtttg
      601 tatgtattac ctgccctatc aatgaggcta gcatctatga gcaacaggtt ttatcgcttt
      661 ttgtgtttta ccatctgtgt gggtttgatt acacaccccg gtcaggcagc gagcgctccc
      721 aaggcgagca acgcctcaga catagcatgg gaggcgggat tggacaagat gagctcagtc
      781 agcgccggcc ttgatcacac ctgtgggatc aagctggatg gtacggtagc ctgctggggt
      841 tcgaatagcg acggcaaaac cgacccgccc gcagaagttt tcagcctaat cagcccaggc
      901 gatgaccaca aaatcagcgc gggcaccttc cataattgcg ggatcaagca ggacggcact
      961 gtcgaatgct gggggaatga cgacgacggc cagtcctcac ctcccgtagg cactttcaaa
     1021 cagatcagcg caggtgggag ccatacctgc ggggtcaagg cggatgatac ggtggcttgt
     1081 tgggggttgg atttagacgg ccagacatcg ccaccccaag gagaacactt taaacaggta
     1141 agcgccggca tcgaacatac ctgtggtgtt aagctggacg aaacgttagc ctgctggggc
     1201 aatcccttcc tccctgaaat ctacgacact cctgacggaa ccttcaaaca aattagtgca
     1261 ggtggctacc ataactgcgc ggtgaaaaag agcgacaata cgctggcttg ctggggagag
     1321 ggcactgaga atactggcat cgacccggat tacggccagg ctatgccacc aggagatgcc
     1381 ttcacccagg tcagcgccgg tggctaccat acctgcggga tcaggacgaa cgggacagta
     1441 gcctgctggg ggtataacta ctacaaccaa gccaccccgc ccgcaggtgt cttcacccag
     1501 gtcagtgcag gcgacttcca cacctgcgga atcaggcagg acgggacggt tacttgttgg
     1561 ggagataatt cttctggtca agcccccaga ggggtcactc cgctattcct gcctctggtg
     1621 agcaggtaag atttcatttt ttcgcggcct gccacagggc ttccatctca tccaggctca
     1681 gatcagtaat cgagcggttt tgggctcgtg tcgcaacctc aatttgggca aaacgactgc
     1741 ggaagcgcgc gttagcctcg cgcagggcgc tctcagcatc tacatcaacc cagcgagcca
     1801 ggttaaccac cgcaaagagc aggtcgccaa tctcagccgc ccggtcatta gccgtacggg
     1861 cagcattgac ttccctgagc tcctcgtgca ctttgtcaat caccccttgg atattcggcc
     1921 aatcgaaacc gacccgaccg gctcgcttct ggtattcctg tgcctgcacc agcgcgggaa
     1981 gcgcctgtgc aacgccatcc agcaagccag cctcagcttt gccattggca ttgcgct
//
LOCUS       DBNGSX010000465         6678 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1639087, whole genome shotgun sequence.
ACCESSION   DBNGSX010000465 DBNGSX010000000
VERSION     DBNGSX010000465.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6678)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6678)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6678
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1639087"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="AC16CB_13725"
     CDS             complement(<1..200)
                     /locus_tag="AC16CB_13725"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR3343575.1"
                     /translation="METKVFARKYYLDWLRVLGILTVFVYHSTRFFNNEDWHVKNLTW
                     YPWLEVWNKFAVTWMMPLMFVIS"
     gene            241..546
                     /locus_tag="AC16CB_13730"
     CDS             241..546
                     /locus_tag="AC16CB_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343576.1"
                     /translation="MQTFRQADARCDNAAGRLVNKVVKSNELVINLILILMRFFTTAF
                     FHPLDVINFPFPMTVACPYDGFGNLRETVVELPPRGNTLKVHAPRKGRITVDALQAL"
     gene            complement(757..1605)
                     /locus_tag="AC16CB_13735"
     CDS             complement(757..1605)
                     /locus_tag="AC16CB_13735"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3343577.1"
                     /translation="MSKTRFIGVIALLAVVMLFIAGCSTGPDPVTVAQQFQEAVNSKD
                     IASALQLLAEDAVFQVDKAPSRSGKAEIENWLTTRAELNFSLEGSPIASDSGVTFESC
                     SISSDMWRYFAVNPMSGACQVTLEDGLITEFAVQFDEDSKVRLSDSAAATSADLVGIW
                     TGGAPPPGGDPHKGEVEDFHLQFAEDGSGRLAVSPDDLLTAPDSDHPGARFKWTYEDY
                     VLTIQNDGPASEGYCLEQDVGTYVIKNVKSVPKNRMQFKLISDSCAFRANTLPRVAAP
                     WNPYVP"
     gene            1893..6602
                     /locus_tag="AC16CB_13740"
     CDS             1893..6602
                     /locus_tag="AC16CB_13740"
                     /inference="COORDINATES: protein motif:HMM:NF044287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nSTAND1 domain-containing NTPase"
                     /protein_id="HIR3343578.1"
                     /translation="MENFAGHTIKGYELLERIGTGGFGAVYRAYQSTMGREVAVKIIL
                     PGYANQPNFIRRFEAEAKLVARLEHLHIVPLYDYWRSPDGAYLVMRWLRGGSVRDALE
                     EGPFGLEAAALLLDQVSSALATAHANGIVHRDLKPSNILLDEEGNAYLADFGIAKDLV
                     QPINTSSADEQLIGSPSPEQARGEPITPQTDIYGLGITLYEILTGTQPFPGLSAIERL
                     FKHINDPIPLIDTLDLRVRDDINNVIQRATAKNPKHRYEDVFAMAAAFRDAAKLSETG
                     RAADLLETLTLREQEILRLVVDGHTNRQIAQDLYIELSTVKWFIRQIYSKLGVRSRKQ
                     AVVRARALKLLMSDVAAEHTEEVNPGIVSVAKLQPVNPYKGLRAFEMADNRDFFGREA
                     FLEKLVNRLASGTSSLSSQDTDQNGDRFLAIVGPSGSGKSSLVNAGLIPALWDGAIKG
                     SERWFMVEMSPGARPLDELEVALLRVAADQAGNIRDQLERDRNGLLRAADLILPRDHS
                     ELLLVIDQFEELFTLVTDEVSRVHFLDLLNAAASDARSRVRIIITLRADFYDRPLHYP
                     EFGELIRNHIETILPLTAEELERAILRPSEQVGVTFEPGLVATIIDAVLYQPGALPLL
                     QYALTELFESRDGRILTHAAYESIGGAAGALARRAEELYHEQEREGQEAIRQMFLRLV
                     ALDDDSDTSPATRQRVARSELIAVVQDEQLLDELVDAYAAYRLLTLDHDPNSRRPTVE
                     IAHEALLSEWQRLCGWLDESRDDLRLRRSLARAAEEWRQAGQDPSFALRGTRLEQFKS
                     WSESTQLALTRGERDYLQASAALRDEWMAAESARQAREAKLEQRARRVLQALVAVFAF
                     AALISLGLSLFAINQRQSALESAAEAQNVALVAGSQAALANHDTDAAIALAWQAVTLN
                     PDSALAQAQLSEAAYAPGTARMLTGNTDMVTWTAISPDNKSLLGGTDDGFVIHWDLAT
                     GQILWEHMVPTESDPPWVQNVAFSPDGQVAAATADDLIMFWQAATGQLIRQIDSTVNR
                     QKITFNPTGDQFATIGAEEHSRLVIWDFNSGEALREYERGSNIEDLVYTADGSAILIA
                     SREGALTLIDAQTGQVIREFEKYQGDNTVVLRYIALGPEGDRVIGAAVDAGLLLWDFM
                     TGELLQNYNYDGVLSLGFNPQDGTILIGDLTVLRTIDPQTGTILRTNTGHSQGILSLV
                     ITSDGSYAITTGVDETVRVWDLQGGQMVRRFIEPQAMLGEVSLSPDGRTMLVGSADGT
                     LILWDMETGEEIRRFVDDQPINAVTFSPDGSKALIGTGNEWEIESGHIILWDVETGAE
                     FRRFEGQPYTVEAVAFSPDGRLAVSAGEGAMAILWDVETGTEVQRFEDYWVDSPWSIE
                     SYWDVQFGPVGQQIFASHSSGAIIGWDVESGQQIRQLVGHSDTVVGIVFSNDGQRLFS
                     AGSDSQIILWDIGTGNVLHRFVNHAGGLGHIQLSPDETLLLGGSLNGTNSLWRVDTGE
                     EIRRYKGGFVNSPHFTPDGRRAVVGYLDGRVELWRIDSTLDELLTWTRENRYIPQLTC
                     EQRELYQVEPLCELNTD"
BASE COUNT         1597 a         1659 c         1801 g         1621 t
ORIGIN      
        1 gagattacga acataagcgg catcatccac gtcaccgcaa acttgttcca cacttctagc
       61 catggatacc aggtcaggtt tttaacgtgc cagtcctcat tgttgaaaaa gcgggtggag
      121 tgatatacga acacggtcag gatgcccagc acgcgcagcc agtccaggta atatttcctc
      181 gcgaaaactt ttgtctccat ttcattcgct ccttataagt cctgccaggt tatgagggcg
      241 atgcagacat tccgtcaggc ggacgcgcgg tgtgacaacg cggctggtcg attggtgaat
      301 aaagtggtga aatccaatga gttggtgatt aatcttattt taatcttaat gagatttttc
      361 acaactgcct tttttcaccc ccttgatgta attaatttcc cctttccaat gacggttgcc
      421 tgtccttatg acgggttcgg caatctccgt gaaacagttg tggagttacc ccctcgcgga
      481 aataccctga aagttcacgc tccacggaaa gggcgaatca cagtagatgc acttcaagcg
      541 ctgtaacact tcaaggaaag tcctagctgc tctacaaggt gcttgtggga tgcaatttgt
      601 actattgaat aaataccact tacacctttt tttcgattgg ctctcccttt ggcataactg
      661 tgattgttcc aaagacagtg cggtctttgg aacaatcatg tgaagaaaga tctttgcgaa
      721 gccagggcgg gatgctccac agcaaaggcc taatgatcag ggtacatacg gattccatgg
      781 agctgcgacc cgtggcaatg tgtttgctct gaaggcgcac gaatcactta tgagcttgaa
      841 ctgcatcctg tttttaggaa cgctcttgac atttttgatc acataagtac ccacatcctg
      901 ctctagacag tatccttcgc ttgccggtcc atcgttctga atggtaagca cataatcctc
      961 ataggtccac ttaaaacgtg cacccggatg gtcggaatca ggggcggtca acaaatcatc
     1021 cggagaaacc gctagcctgc cgctcccgtc ttcagcaaat tggagatgga agtcttctac
     1081 ctcaccctta tgaggatccc ctcctggtgg cggagcccca cctgtccata ttcccaccaa
     1141 atcggcgctc gtagcagccg ccgaatcaga caaacgtacc ttcgagtcct cgtcgaattg
     1201 aacagcgaac tctgtgataa gaccatcttc gagtgtcact tggcaagcac cggacattgg
     1261 attgaccgca aagtagcgcc acatgtctga gcttatcgag cagctttcaa atgtcacgcc
     1321 agaatccgag gctattgggc ttccttcaag actaaagttg agttcagccc gtgtcgtcag
     1381 ccaattctca atctccgctt tgccggagcg ggacggcgcc ttgtcaactt ggaaaacagc
     1441 atcttctgcg agcaattgga gcgcagatgc aatatcttta gaattgaccg cctcttgaaa
     1501 ctgctgggca acagtcactg gatcggggcc ggtactacat cctgcaatga acaacattac
     1561 gactgccagt aaagctataa caccgataaa acgagtcttt gacataaggt tttcctttcc
     1621 ctgattggaa tgatttggtt tgttctcgat ctgagaagcc actatccact cgatattttc
     1681 tggggtgcct tttgtttccc catgaaaata gacaaacggc ttgatcgtgc aatcaggtta
     1741 gcatcgttag catcgtaacg gagggagggg aaagggaggg ttttatttct aatgtcgaga
     1801 aatcagatat actatagtca ctacattagg attgtttggt tgggatggta gatttcactc
     1861 cctactaaat gaactctcct gatattgctg ttatggaaaa ctttgctgga cacacaatta
     1921 agggttatga actgcttgag cgaatcggta cagggggatt tggcgctgtt tatcgcgctt
     1981 accaatctac catgggccgt gaagttgcgg ttaagatcat cctgcctggc tatgccaatc
     2041 aaccaaactt tatacgtcgt tttgaagcag aagcaaaact ggtagctcga ctggagcatt
     2101 tacacatcgt gccactttat gactactggc gtagtccaga cggcgcatac ctggtcatgc
     2161 gctggctgcg cggaggtagc gttcgggatg cgctcgaaga ggggccattt ggcttggagg
     2221 ccgcggcatt gctgctagat caggtgtcat ctgcactggc gactgctcat gccaatggca
     2281 ttgtccaccg ggatttaaaa ccatcgaata tcctgcttga cgaagaaggg aatgcctatc
     2341 tagctgattt tgggattgcc aaagatctag ttcaaccgat taatacctcc tctgcagatg
     2401 agcagctgat tggctcacct tctccggagc aggcgcgtgg cgaaccgata acaccgcaaa
     2461 cggatattta cggtctggga ataaccttgt atgaaatcct gaccggcaca caacccttcc
     2521 ccggtttgag cgcgatcgag cgcctgttca agcatatcaa tgaccctatc ccgttgatag
     2581 atacgctcga tctacgcgta cgtgatgata taaacaatgt tatccaacga gccactgcca
     2641 agaatccgaa gcatcgctat gaggatgtgt tcgcgatggc agccgcattc cgggatgcag
     2701 cgaagttaag cgaaacgggc cgagctgctg acttgctgga aacattgacc ctgcgggagc
     2761 aggaaatctt gcgcttggtt gtcgatgggc acaccaaccg gcagatcgcg caagacttgt
     2821 atatcgagct ttccactgtg aagtggttca tccggcagat ctacagcaaa cttggggtac
     2881 gcagccgtaa gcaggctgtt gtgcgagccc gagcattaaa acttttgatg agcgatgtag
     2941 ctgcggagca cactgaggag gtaaaccccg ggattgtcag tgttgcaaag ctgcagccgg
     3001 taaatcccta caaaggcttg cgagctttcg aaatggccga taaccgcgac tttttcggcc
     3061 gtgaggcatt tctagaaaag ctggttaaca ggctagcttc aggcacttcc agcttatctt
     3121 ctcaagatac tgatcaaaat ggtgatcggt tcctagctat tgtcggccca agcggcagcg
     3181 gtaaatccag cctagtgaat gctggattga tcccggcgct ctgggatggt gccataaagg
     3241 ggtcggagcg ctggttcatg gtagaaatgt ccccgggtgc tcgaccgctg gatgaattgg
     3301 aagtagcact gctccgcgtg gctgctgatc aggcgggtaa cattcgcgac cagttggaaa
     3361 gagatcgaaa cggtttgttg cgggccgcag acttgatcct gcctcgagat catagcgagt
     3421 tgctattggt gatcgaccag tttgaggaat tgttcacttt ggtcacggac gaagtctctc
     3481 gggtgcattt tttagatctc ctcaatgcgg cagccagcga cgctcgcagc agggtgcgca
     3541 tcatcatcac tctgcgggcc gacttctacg atcgcccgtt acactatccc gaatttggcg
     3601 aattgatccg caaccacatt gaaacgatcc tgcccttgac tgccgaagaa ttggaacgtg
     3661 ccattttgcg accgtctgaa caagtaggag taacgtttga gccagggttg gttgctacca
     3721 tcatcgatgc cgttctctac caacccggag ccctaccctt gctgcaatac gcgcttacag
     3781 agttatttga aagccgcgat gggcgcatcc tgacccacgc ggcctacgaa tctatcggag
     3841 gagccgcggg tgccttggcc cgccgcgcgg aggaactgta tcacgaacag gaaagggaag
     3901 ggcaggaggc gatccggcag atgttcttgc gcctggtagc gttagatgac gattctgata
     3961 cctcccctgc cacccggcaa agggtggctc gttctgagct catcgctgtc gttcaagacg
     4021 aacagctgtt ggatgagctt gtcgatgctt atgccgccta ccgtctgctg acgctcgacc
     4081 atgacccgaa cagccgccgt ccaacggtcg agatcgccca tgaagccctg ttgagcgaat
     4141 ggcagcgtct gtgcgggtgg ctggacgaaa gccgcgacga tctccgcctg cgacggagtc
     4201 tggcgcgggc ggcagaggag tggcgacaag caggccagga tcccagcttt gccctgcgcg
     4261 gcacgcgcct ggagcagttt aaatcctgga gtgagtccac acagttagct ttaacgcgag
     4321 gcgagcgtga ttacctgcaa gccagcgctg cgctgcgtga cgagtggatg gctgccgagt
     4381 ccgcacgcca ggcgcgcgag gcgaaactgg aacagcgcgc ccggcgggtg ttacaggcgc
     4441 tagtggctgt atttgccttt gctgcgctaa tcagtttagg gctttcatta ttcgcaatta
     4501 accaacgcca atctgcctta gagagcgcgg ctgaggctca aaatgtagcc ttagtagcgg
     4561 ggtcgcaggc tgccctcgcc aatcatgaca ccgacgccgc catcgcactt gcctggcagg
     4621 ctgtaacgct taaccctgac tcagccctcg ctcaagcgca gttgagcgag gctgcctacg
     4681 cgccgggcac agctcgcatg cttacaggta acactgatat ggtgacctgg acagccatca
     4741 gtcctgacaa caaatcacta ttgggtggaa cggatgatgg gttcgtcatt cactgggatt
     4801 tggctacagg ccaaatattg tgggagcaca tggtgcctac agaatcagat ccaccctggg
     4861 tccagaatgt cgccttcagc ccagatgggc aggttgccgc cgctactgcc gatgacctga
     4921 tcatgttttg gcaagcagcc acgggccagc tcatacgtca aatcgattcc accgtcaatc
     4981 gtcaaaaaat tacttttaat cccacgggtg atcaatttgc aaccatcggc gcagaagagc
     5041 acagccgact agtaatatgg gacttcaatt caggagaagc tctgcgcgaa tacgaacgag
     5101 gatcaaacat cgaagatctc gtgtacaccg ctgatggatc agcgattttg atcgcaagta
     5161 gagaaggtgc cttgactttg atagacgcgc agacaggaca ggttatccgt gaatttgaga
     5221 aatatcaggg ggacaacaca gtcgtcctgc gttacatcgc tctcggtccg gaaggcgacc
     5281 gggttattgg tgctgctgtt gatgcaggtt tgctgctttg ggatttcatg acaggtgagc
     5341 tgttgcaaaa ttataactac gatggtgtcc tatctttagg atttaatccg caagatggca
     5401 ctattttaat tggcgacctc actgttctcc gcaccatcga tccgcaaacg ggtacgatac
     5461 tacgtaccaa tactggccat agccaaggca tacttagtct ggtgattacc tccgatgggt
     5521 catatgccat cacgaccgga gttgatgaga ccgtccgtgt gtgggactta cagggcggac
     5581 aaatggttcg tcgatttatt gagccgcaag cgatgttagg agaagtctct ctcagtcctg
     5641 atggtcgaac tatgctagtc ggttcagcag atggcactct catactttgg gatatggaaa
     5701 cgggtgaaga aattcgccgt tttgtggatg accagccgat caatgcagtg acattctcac
     5761 cggacgggag taaagccctg attggaacag gtaatgaatg ggagattgaa tctggacata
     5821 tcatcctgtg ggatgtggaa acgggggcag aatttcggcg ctttgagggg cagccttaca
     5881 ctgtagaggc cgttgcgttc agccctgacg ggcgattggc tgtatctgct ggcgaaggtg
     5941 ccatggccat cctgtgggat gtggagacgg ggacagaagt tcagcgcttt gaagattact
     6001 gggtggacag cccatggagt atcgaatcct attgggatgt gcaattcggt ccggttggtc
     6061 agcagatatt tgcctcgcat tcaagcggag cgatcattgg atgggatgtg gaaagcggcc
     6121 aacaaatacg acaactggtt gggcatagtg atacagtagt aggaattgtt ttcagtaacg
     6181 atggtcaacg gctcttttca gcaggctcgg attcccaaat aattctctgg gacataggta
     6241 cagggaatgt attgcatcgc tttgttaacc acgctggtgg attagggcat atccagcttt
     6301 ctcccgatga aacattgtta ctaggtggca gtctaaacgg caccaactcc ctttggcgag
     6361 tagatactgg tgaagaaatc cggcgttaca aaggtgggtt tgtaaatagc ccacacttta
     6421 ccccggatgg gcgtcgcgct gtggtcggct atctcgatgg cagggtggag ttgtggcgca
     6481 ttgactcgac attagatgaa ctgctcacct ggacacggga gaatcgctat attcctcagt
     6541 tgacctgcga acagcgtgaa ctctaccagg ttgagccgct ttgtgagctc aatacagatt
     6601 aaaagccacc ccaccacaag cctgattggg gtagggtggc gcacgttgac gttcgacatg
     6661 tgcagcaacg tgcgcaac
//
LOCUS       DBNGSX010000466         4514 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1639222, whole genome shotgun sequence.
ACCESSION   DBNGSX010000466 DBNGSX010000000
VERSION     DBNGSX010000466.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1639222"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="AC16CB_13745"
     CDS             <1..299
                     /locus_tag="AC16CB_13745"
                     /inference="COORDINATES: protein motif:HMM:NF049042.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR3343579.1"
                     /translation="ALLALPTPEAQEAALRTILKQDLNVRQTEEIVRKLSGLRPVSTP
                     KANPSPELTALEERLRTKLGTRVKLNRSHKGGTLVIHFYSDEELDALLETLLQE"
     gene            296..1912
                     /locus_tag="AC16CB_13750"
     CDS             296..1912
                     /locus_tag="AC16CB_13750"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR3343580.1"
                     /translation="MTRVLICANIYTLNPQQPHATAIVIDGERILRLGARQEILSEFA
                     GDGHRVEILDARHQTLIPGLIDAHFHLEQYALAQQMVDCETPTRAECERRVAERAQQM
                     PPGEWVLGHGWNQNDWPEGFGNNTDLDAVSPKNPVYLTAKSLHAAWANSLALEAAGIG
                     AHTSDPPRGRIGRNEHGEANGILFEGAMELVQQALPEPTPQQVAQAIRDVLPKLARMG
                     LTGLHDFDRRRCFSALQILHQREELTLRVVKSIPMEDLTYAAVLGLRTGFGDLYLRIG
                     GIKAFADGALGPRTAAMVQPYEGEVDNRGMLLLDSEELFEQGRMAIDNGLCIAVHAIG
                     DRANHEVLNAFTQLRAYEKEQNTTHSFPRFPLRHRIEHVQVIHPDDTRRLAELGIIAS
                     MQPIHATSDMLMADKYWGSRAALAYAWRTQLEHGASLAFGSDAPVESPNPFWGLHAAV
                     TRRRPDGSPGPQGWYPAQKLTISDALLGFTSGPAYASYLENQLGKLMPGFLADLTILD
                     NDPFRCDPATIKEIRPLATMVGGKWIFREV"
     gene            complement(1937..2626)
                     /locus_tag="AC16CB_13755"
     CDS             complement(1937..2626)
                     /locus_tag="AC16CB_13755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3343581.1"
                     /translation="MDSPLVLFADDDFDSQQSVCLVLEEAGMNVVFAENGSHALDLWH
                     KHPVDAVVLDVMMPDVDGLDACRQIRRESNVPIILLTARGREEDIIAGFNSGADDYIV
                     KPFRPRELVARIGALMRRTAGSSWYNRKQLAFDSLILDLESRCVIQRDHQVEISPMEF
                     RLLKYLMQNAGNVISKEELLHDVWGYQASSGDMNLIEATVRRLRQKVESDPSRPRYIQ
                     TVWGAGYRFGD"
     gene            complement(2860..>4514)
                     /locus_tag="AC16CB_13760"
     CDS             complement(2860..>4514)
                     /locus_tag="AC16CB_13760"
                     /inference="COORDINATES: protein motif:HMM:NF013508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sortase domain-containing protein"
                     /protein_id="HIR3343582.1"
                     /translation="TKTDGRTTVSPGQTYTYTITVKNLGSITAESYVITDTLSPYLTY
                     IDDDSGIEPVKSGDTYIWEVDDDLLKLEENSFEVEVKVDSSLPSASTQIVNKVNVATS
                     SPEANKTNNEATDTNNGTGTHNVSITVSVSPSSVRTGQNATYTIKVSNSGSAAVTDVE
                     MTDTFSQYLDIVSASTNKGSRDINESSRRVTASIGTLNAGQSATITVVAKVNNVARAN
                     LTVSNSASVTYKFGGSSFTKSSSSASFQIVFSSTLPGTGGIELPTKSAETPGLQGYLV
                     ALISAILLAMVGFVALWMGVQAKASQSNWASFTLRFSLMFFVSAVFFGLAAWGLYALT
                     GNPSASGLMARVKPEWVLKATPVMPEDPPWVWSPIEGEPEVLPDFPIPEPTDVAPDSN
                     GNSPDTSPVTRIVVPILGIDTVVKYVPYDGFTWLIGGLQHEVAWMGDTSWPGLGGNTG
                     LAGHVSLRNGRDGPFRYLDSLQPNDEIMLYTEENLYIYKVNARSVVSDSDMSVIQPTE
                     NPRLTLITCINFDRENGVYRDRLIVQADLLSVTSLAKAIRGN"
BASE COUNT         1163 a         1186 c         1093 g         1072 t
ORIGIN      
        1 gcgctttgtt agccctcccc acacccgaag ctcaagaagc ggcgctgcgc acgatactca
       61 aacaggattt gaatgtgcga cagaccgaag aaatcgtgcg caagctcagt ggactccgtc
      121 ctgtgtccac gcccaaagca aatccatctc cagagctgac tgccttagag gaaagactgc
      181 ggacgaagct cggcacacgg gtaaagctca atcgctcgca taaagggggc accctggtca
      241 tccacttcta ttctgatgag gagctagacg ctttactcga aacactgctg caagaatgac
      301 cagagttctt atctgcgcaa atatctatac gctcaacccg cagcagcccc atgcaaccgc
      361 catcgtcatc gatggcgaac gcattttgag gctgggtgcg cggcaggaga tattaagcga
      421 attcgccgga gatggacatc gtgttgagat cctggatgca cgccatcaga cgctcatccc
      481 cggcctgatc gatgctcatt ttcacctaga acaatatgct ctggcacagc aaatggtgga
      541 ctgcgaaact ccaacgcgtg ctgagtgcga gcgcagggtc gctgagcgag ctcagcagat
      601 gccaccggga gaatgggtgt tagggcatgg ctggaaccaa aatgattggc ctgagggctt
      661 cggcaacaac accgacctcg acgcagtatc tcccaaaaac ccagtatatt tgacagccaa
      721 atcgctgcac gctgcatggg caaacagcct agccttggaa gctgcaggca tcggcgcgca
      781 cacttccgac ccacccaggg gacggatcgg ccgaaatgag catggcgagg caaacggcat
      841 ccttttcgaa ggggcgatgg agttggtcca acaagctctc cctgagccca ccccgcaaca
      901 ggtagcccag gctattcggg atgtcttacc aaaattggcg aggatgggcc tgaccgggct
      961 tcacgatttc gatcggcggc gttgtttttc ggctctgcaa atcttacacc agcgcgaaga
     1021 gctaactcta cgcgtggtca agagtatccc aatggaagat ctgacctatg ctgccgtgct
     1081 tgggttgcga actggcttcg gagacctcta cctgcgcatc ggcggcatta aagctttcgc
     1141 tgatggagct ctcggcccgc gcactgcagc catggtgcaa ccctatgaag gtgaggtcga
     1201 caaccgtggc atgctcttat tagactcaga agagctgttc gaacagggtc gcatggcaat
     1261 tgacaatggt ttatgtatag cggtacatgc cattggggac cgcgccaacc acgaagtgct
     1321 gaatgcattc acccaactgc gagcgtatga gaaagagcag aatacaacac attctttccc
     1381 tcgatttccc ttgaggcacc gcattgaaca tgtccaggtg attcatccag atgacactag
     1441 acgcctagca gagttaggca tcatcgcctc gatgcaaccc atccacgcga catcagatat
     1501 gctcatggca gataaatatt gggggtccag ggctgcattg gcgtatgctt ggcgtacaca
     1561 attagaacac ggggcatccc tagcctttgg gtcggatgcg ccggttgaat cgcccaaccc
     1621 gttctggggt ttgcacgccg ctgtgacccg ccgacggccg gatggaagtc ctggtccaca
     1681 aggttggtac ccggctcaaa agctcactat ctctgatgcg ttgctgggtt ttacttccgg
     1741 ccctgcctat gcctcatacc tagaaaacca actaggcaag ttaatgcctg gtttcctggc
     1801 cgatctcacc atacttgata atgatccatt tcgttgtgat ccagctacta taaaagaaat
     1861 tcgcccccta gcaactatgg tcgggggcaa atggattttt cgagaggtat aaatttaaag
     1921 gtctattcag gcacaattaa tcaccgaacc ggtagcccgc cccccacacg gtttggatat
     1981 aacgcggccg agaaggatcg gactccacct tttggcgcaa gcggcgaact gtcgcctcga
     2041 tgagattcat gtctcccgaa gaagcctgat atccccaaac atcatgaagt aattcttcct
     2101 tgctaattac gtttccagca ttttgcatga gatatttgag caggcggaac tccatcggag
     2161 aaatttcaac ctggtgatcg cgttggatta cgcagcgcga ttcaaggtct aagatcaggc
     2221 tatcaaatgc caattgcttg cggttgtacc aggaactccc tgctgtccgg cgcattaaag
     2281 ccccgatcct agcaaccagc tcgcgagggc gaaaaggctt gacaatgtaa tcgtctgcgc
     2341 cagaattaaa accagcaatg atatcttctt ctcttccgcg agcggtcagc aagataatcg
     2401 gcacattcga ctcacgccta atttgccgac aggcgtcaag accatccacg tctggcatca
     2461 tgacatccag cacaacagcg tccaccgggt gtttgtgcca gagatccaag gcatgagagc
     2521 cattctcagc gaagacgaca ttcatccctg cctcttctaa aaccaaacac acagattgtt
     2581 ggctgtcgaa atcatcgtct gcaaagagaa cgagtggaga gtccacctta ttcctccaga
     2641 tattaaaacc aatgtaattt gagactaagt gatccaaata tcaaaacgaa aaaatatctg
     2701 ttttgttaca gtgtaacaaa acagatatgt tgggtttttt tagattggga gtgtgaggtg
     2761 ctagattaga tgggtctaat cccggaattt tcttggatat tctgaatgag ccaaattaga
     2821 agccaccagg tatcaccaat gaaagatatt ctggaatgct caatttcctc gaatggcttt
     2881 tgccaaggat gttacactca ggagatcggc ttgcacgatt agacggtctc ggtacacccc
     2941 attctctcga tcgaaattta tacaagtgat caaggtcagc ctcgggtttt ccgtcggctg
     3001 gatgactgac atatcagaat cggaaaccac gcttcttgcg ttcaccttgt aaatatacaa
     3061 attttcctcg gtgtaaagca tgatctcgtc atttggttgc aatgagtcca gatagcggaa
     3121 ggggccatcc cgcccatttc gcaagctcac gtgaccagct aacccagtat taccacccaa
     3181 tcctggccag gaagtatccc ccatccaggc gacttcgtgt tgcaatccac caattaacca
     3241 ggtaaatcca tcataaggca catacttcac caccgtatcg atacctagaa tcggcacaac
     3301 gatacgggta actgggctgg tatctggcga attgccattt gagtctggag ccacatctgt
     3361 gggttcagga atagggaaat ccggcaggac ttccggttcg ccttcaatag gagaccatac
     3421 ccagggtgga tcttctggca ttaccggagt cgccttcaat acccattccg gcttcactcg
     3481 cgccattaac cctgaagcag acggattacc cgttagcgca tataaacccc aggctgccag
     3541 accaaaaaac accgcggata caaagaacat caagctaaag cgaagcgtaa aactggccca
     3601 attagattgg cttgctttgg cttggactcc catccataaa gcgacaaatc caaccatagc
     3661 cagcaggatt gcactgatca gggcaactaa gtaaccctgc aatccaggtg tttccgcaga
     3721 tttggtaggc aattctattc caccggtgcc aggtaaggtg gacgaaaaga cgatctgaaa
     3781 tgaagcagac gacgaagatt ttgtaaagct cgagcccccg aatttatacg ttacgctagc
     3841 tgagttgctt accgtcagat tcgcccgggc aacgttgtta acctttgcaa caactgtaat
     3901 ggtcgccgac tggccagcat ttaaagtgcc tatcgacgcc gttaccctac gagaagattc
     3961 attgatatcc cggctacctt tgttggtcga agcgctgaca atatccagat actgggaaaa
     4021 ggtatccgtc atttctacat cagtcaccgc tgccgaccct gaattgctga ccttgatggt
     4081 gtacgtagcg ttttgccccg tacgcactga actgggtgat accgacacag ttatggagac
     4141 attatgcgta cccgtgccat tgtttgtgtc tgtggcttca ttgttcgttt tgttagcctc
     4201 gggactggaa gtagccacgt ttaccttgtt tacgatctgg gtagatgccg atggtaagct
     4261 gctgtctacc ttcacctcga cctcgaaaga attttcctct aatttcaata aatcatcatc
     4321 cacttcccag atgtaggtat ctcccgattt tactggctca atccctgaat catcgtcgat
     4381 ataggtaaga taaggggata atgtatctgt gattacgtag gactcggcgg taatagatcc
     4441 aagatttttc acagtaatcg tataagtata ggtctgccca ggagatacgg tcgtcctccc
     4501 atcggttttt gtta
//
LOCUS       DBNGSX010000467         3898 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1154346, whole genome shotgun sequence.
ACCESSION   DBNGSX010000467 DBNGSX010000000
VERSION     DBNGSX010000467.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1154346"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="AC16CB_13765"
     CDS             complement(<1..473)
                     /locus_tag="AC16CB_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014649962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR3343583.1"
                     /translation="MEKLLTTKLYIPRTRPDLVPRPRLIERLNEGLHRKLTLICAPAG
                     FGKTTLISEWVAGCKRPVAWLSLDEGDNDPIRFHAYLVAALQTIEAGIFENVQAMLRT
                     SQPPPIESVLTELINEVVTLSQDFVLVLDDYQVIEDRSIDESLTFLLDHIPPVMHL"
     gene            complement(635..1639)
                     /locus_tag="AC16CB_13770"
     CDS             complement(635..1639)
                     /locus_tag="AC16CB_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium chain dehydrogenase/reductase family
                     protein"
                     /protein_id="HIR3343584.1"
                     /translation="MKYKRVVVSRYGGPEVLQIVEDELPEPQPGEVRLRILAAGVAWG
                     DVLKREGFSIGVRPPFTPGYDIVGVVDKLGEGVSTFKVGQIAAALPLFGGYAELICLP
                     ALELVQVPSGLEPAKAVCLVMNYVVAHQLLHRVARVKAGERILIHGAAGGVGTALLQL
                     GKLAELEMYGTASGGKHETITSLGGIPIDYKTEDVLGRILDLTGDGVDVIFDPVGGSQ
                     TWQSYRMLRRGGRLVIYGAHSILTDGKLKTITGFVLGPILNLLPDHRAVMSYNITRPK
                     YSKPEWCREDLSKLFALLSQERIEPVIAERMPLLEAARAHQLLEEGAVVGKLVLVPNP
                     "
     gene            complement(1665..2222)
                     /locus_tag="AC16CB_13775"
     CDS             complement(1665..2222)
                     /locus_tag="AC16CB_13775"
                     /inference="COORDINATES: protein motif:HMM:NF021886.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3343585.1"
                     /translation="MSLKYAMLGFLSYQPFSGYELKATFDDSVRHFWPADQAQIYRTL
                     AELTKDGWATMEVVPQEDRPNKKVYYITEAGREALRGWLAKPLPLQDHRSAELIQVFF
                     AGQLTDEQALAMFERFAQEYRAGLASLYQIPEQIKAYEDMVGSSREAFFWMLTLEHGI
                     HSTRAVLEWLESVIERLKNKQVPQG"
     gene            2552..3181
                     /locus_tag="AC16CB_13780"
     CDS             2552..3181
                     /locus_tag="AC16CB_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008099432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HIR3343586.1"
                     /translation="MKKLDLRKELKHLYNPSAKRVEVVEVPRFKFVMIDGAIEPGHEP
                     GNSPKFQEAMQALYGAAYTLKFMSKLRKEDPVDYPVMALEGLWWVENGQFSFTRKDNW
                     MYTLMIMHPDHITQAMFEDAVQQVRAKRGDSPALSLLRWEEFEEGLCIQTMHIGPYAT
                     EPATLEKMDAFARENNYTLHGKHHEIYLGNPQRAQPEKLKTVLRHPISK"
     gene            3470..>3898
                     /locus_tag="AC16CB_13785"
     CDS             3470..>3898
                     /locus_tag="AC16CB_13785"
                     /inference="COORDINATES: protein motif:HMM:NF019632.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3343587.1"
                     /translation="MPNPTTKRELLDEIHAEREKLDRFLAPMTPEQMIQPGAIEEWSV
                     KDVLAHLAEWEVLLLIWYNAGRRGENPPLPAEGYGWEQEDDLNQNIYEKYRGWSLDEV
                     LAYYRKSFQETVAAVQAMTEEELFTPGRYAWTKNNTLADYV"
BASE COUNT          992 a         1019 c         1009 g          878 t
ORIGIN      
        1 aggtgcatca caggtggaat atggtcgagc aagaaagtga gtgactcgtc aattgaacgg
       61 tcttcaataa cctggtagtc atcgaggact aatacaaaat cctgggaaag ggtaactacc
      121 tcattgatta gctctgtcag caccgattca atgggtggtg gctgcgaggt acggagcatc
      181 gcttggacat tttcaaagat acctgcttca atggtctgca gggctgcaac aagataagcg
      241 tgaaagcgga tgggatcgtt atccccttca tccagcgaca gccaggcaac cggacgcttg
      301 caaccagcga cccattcgct gatcagtgtg gttttaccaa agccggcagg ggcacagata
      361 agtgtcagtt tgcggtgcag accctcgttc aatcgctcga tcagccgcgg gcgcggaacg
      421 aggtctggac gggttcgggg gatatagagt tttgtggtta gaagtttttc cacgattagc
      481 cttgattttc gtggtttaaa atgattatag catgacgaat ctcataaagg tttcaattac
      541 gctcaattgc aaacatcaac aatatgtgat aaatctgagg cacggaagaa cgagaaagcc
      601 gcttgtgaag atatagggcg ggctagcggg ctgcctatgg gttgggtacc aggaccagtt
      661 tgcccaccac agcgccttct tcaagcaatt ggtgagcccg ggccgcctcg agcaatggca
      721 ttctctcggc aataaccggc tcgatccttt cttgtgacag gagggcgaac aacttggata
      781 gatcctcacg gcaccattcg ggtttgctat attttggtct tgtgatgttg tagctcatta
      841 ccgccctatg gtcaggtaag agattcaata ttggacccaa aacaaaaccc gtgatggtct
      901 tgagcttgcc atctgtgaga atcgaatgcg ccccatatat aaccaaccga ccgcccctgc
      961 gcaacattcg ataggattgc caggtttggc tgccgccaac cggatcgaat atcacatcaa
     1021 ccccgtcccc ggtcaaatcc aggatgcgcc ccaagacgtc ctctgtcttg tagtcaattg
     1081 ggattccacc caagctggtg atcgtttcgt gtttgccgcc ggaggctgtt ccatacattt
     1141 ccagttcggc taacttacct agctggagca gggctgttcc cacgccccct gccgcaccgt
     1201 ggatcaagat gcgctcgccg gctttcacgc gagcaacgcg gtgtagcaat tggtgcgcaa
     1261 ccacataatt cattaccagg caaacagctt tggcaggttc caagcccgag ggcacttgta
     1321 ccagctccaa ggcaggtaaa caaattaact cagcatagcc gccaaataga ggtaaggcag
     1381 ccgcgatttg tcctaccttg aacgttgaga ccccttcacc aagcttgtcc acgacaccga
     1441 caatatcgta gcctggcgtg aacggtgggc gtacgccgat gctaaacccc tcccgcttca
     1501 atacgtctcc ccaggccacg cctgctgcca gaatcctcag gcgcacctcg cccggttgag
     1561 gctctggaag ctcgtcctcg acgatctgga gaacttctgg cccgccatag cgggatacaa
     1621 ccacgcgttt atatttcatt ttttatcctc caagtgttag ccgactatcc ctgtggaacc
     1681 tgcttgttct tcaatcgctc gatcacgctt tccagccatt ccaaaacagc gcgggtagag
     1741 tggataccat gctccaatgt gagcatccag aaaaaagcct cccgcgacga gccaaccata
     1801 tcctcataag ctttgatctg ctcaggtatc tggtataagg acgccagccc agcgcgatac
     1861 tcttgggcga aacgctcgaa catcgccagg gcctgctcgt ctgtgagctg cccggcgaaa
     1921 aatacctgga tcagctcagc gctgcgatga tcctgcagcg gcaggggctt agccagccag
     1981 ccgcgcagcg cctctcgccc tgcttcggta atgtaataga ctttcttatt aggacgatct
     2041 tcttgaggta ctacttccat ggtcgcccag ccatctttcg tcagctctgc cagggtgcga
     2101 tagatttgtg cctggtcagc cggccagaag tgacgtacgg aatcgtcaaa ggtagccttg
     2161 agctcgtagc cagagaacgg ttggtagcta aggaagccca gcatggcgta ttttagagac
     2221 atgggtaact ccgattggtg gtttgtatca taattttcgg ttttactggc tattcttgcc
     2281 tgaatggatg cacttcacat cacaatactt cgagacaaat tattagatgt atcttatata
     2341 ttatctatat tatatatgtc taatcatata ataaaaatga tatcttgtca agaggaaaaa
     2401 ctgattcgat aattaacctg ggactcgagc aaaatttggc gtagaatcca attaaagatg
     2461 attgtgaaca acaccgcaat tggctgaaca ccgccatcat gctgccgtag caccggctgc
     2521 aaacttggaa ctgcttgcag gagagatcac catgaaaaag ctggatttac gcaaagagct
     2581 gaaacacttg tacaacccct cagccaagcg tgtggaggtg gtggaagtgc cgcgcttcaa
     2641 attcgtgatg atcgacgggg cgatcgagcc gggtcacgag ccaggtaact ctcctaaatt
     2701 tcaggaagcc atgcaggcct tgtacggcgc tgcctacaca ttgaagttta tgtccaagct
     2761 gcgcaaagaa gaccctgtgg actatcctgt catggcattg gaagggcttt ggtgggtcga
     2821 gaatggccag ttcagcttca cccgcaagga caactggatg tataccctga tgatcatgca
     2881 ccccgatcac atcacccagg ccatgttcga ggacgccgtc cagcaagtgc gggcgaaacg
     2941 cggcgatagc cctgccctca gtcttctgag gtgggaggaa ttcgaggaag gcttgtgtat
     3001 acagaccatg cacatcggtc cctacgctac cgagccagcc acgttagaga agatggatgc
     3061 cttcgcccgg gaaaataact ataccctgca tggcaaacac catgagatct acctgggcaa
     3121 cccacagcga gcacagcccg agaagctcaa gaccgtgctc aggcacccaa tatcaaaatg
     3181 aaaagctgtt ccgtggtcat cacgagcatc cctaacgcag catgcttcag gcgcgagcgg
     3241 tattgatgga catcgcgagg cctggcgcac tttgccaggg cgtggcgatc tcggacatta
     3301 agatggctta gtctttacgc tcctcgccat gaccaagttg gagtgtgtca tttgcaaacc
     3361 tgccgtccaa aggacgtcct ggaaaagcgc atgctagatt tgatcagcat ccaggccaag
     3421 cacgtgagaa aagtaattta agctgctgaa aaaatctcag gaggaaagta tgccaaaccc
     3481 aaccacgaag cgtgaactct tagacgaaat ccacgctgaa cgagagaaac tggacagatt
     3541 cctggcaccc atgacgccag agcagatgat ccaacccgga gctattgaag agtggtcggt
     3601 gaaggatgtg cttgctcacc tggctgagtg ggaggtcttg ctcctgatct ggtacaacgc
     3661 cggtcggcgc ggcgaaaacc cacctctccc tgctgaaggg tatggatggg aacaggagga
     3721 tgacctcaat cagaatatct acgaaaaata ccgaggctgg tcgctggacg aagtgctggc
     3781 atattaccgc aaatccttcc aggagactgt ggcagccgtg caggccatga cagaagaaga
     3841 gttgttcacc cccgggcggt atgcctggac aaagaacaac acactggcgg attatgta
//
LOCUS       DBNGSX010000468         8438 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1154369, whole genome shotgun sequence.
ACCESSION   DBNGSX010000468 DBNGSX010000000
VERSION     DBNGSX010000468.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8438)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8438)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8438
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1154369"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            404..2227
                     /locus_tag="AC16CB_13790"
     CDS             404..2227
                     /locus_tag="AC16CB_13790"
                     /inference="COORDINATES: protein motif:HMM:NF012784.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIR3343588.1"
                     /translation="MESNNALPGQGVEPESKESTEQKPEERPTTDLSTMAILLEEQGL
                     VDLPKTGEVRSGVIAAIGENEILVSVGTKSEGVISGRELDGIPAEERAEFAVGTEIPV
                     YVVTPEDTNGNVVLSYLRAREEKDWHDAEQMLEDEDGFESKIIGYNKGGLLVPLGRLR
                     GFVPASQISLVRRATLGGDSPDQRWGKMVGEPIKVRVIEVDRERHRLILSERAALQET
                     RETLKDRLLDELSEGDVRTGRVTSVADFGAFVNIDGADGLVHLSEISWERINHPNEVL
                     KVGQDVQVKVISVDHERRRIGLSIRQLQEDPWLKKIKGMREGQLVEATVTHLTKFGAF
                     ARLDEELEGLIHISELSEQRINHPKEVVKEGEVVTLRVIKIDPERRRIGLSRRKVDSP
                     AYSDLDWKMALAEEVQEVQETGAPQPSAEDEGQETIVSQVAAEEIVQEAEAIAETSVE
                     EETVEVMAEAKAEEETAEAGVEAQAEEEAAEPAVEASNEVVAEAKTEEETAEAEDEAQ
                     AEEEAAEPEVKASNEVVAEAQAEEETAEAEDEAQAEEEAAEPEVEASNEVVAEAQAEE
                     ETAEAEVEAQAEEKAAEPEVEASNEVVAEAQAEEETASKAE"
     gene            2540..5032
                     /locus_tag="AC16CB_13795"
     CDS             2540..5032
                     /locus_tag="AC16CB_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR3343589.1"
                     /translation="MERFTQRARRVLSLAHQEAERMRHNYIGTEHLLLGLIREEGGVA
                     GRVLRELGLEPERVQEIVERLTGTGQYRGGKLDLSPGTQQVLEYAVDEARRMGHHYIG
                     TEHLLLGLVRYGEGVAMDVLRKLGVTPEQIRRQTRRVLQESTASRRTSVGESQKTAKQ
                     ESQKPKTPMVDQLATDLTGLAEEDKLDPVIGRQMEIERVIQILARRTKNNPALIGEPG
                     VGKTAIVEGLAQRIVEGDVPAPLLSKRVLQLDVGSLVAGTMYRGQFEERLKRVIEELK
                     SSGAILFIDEVHMLVGAGAAGSSVDAANILKPALSRGELQVIGATTLDEYRKHIESDA
                     ALERRFQPIMVEEPSEEETIEILRGIRSAYEEHHRLTISDEALDAAVHLSARYVTERF
                     LPDKAIDLIDESSSRVRMYKSDAAQAAKELMRELRELRTNHAIALEDNRYDDAQDYLD
                     RQENLEMQLERMRTVWDRASSPVVSADDIAEVVSMWTGVPVMQIAKEESQRLLEMEKE
                     LGSHIVGQEEAIQAISKAVRRARAGLKDPRRPIGSFIFLGPTGVGKTELTKAMARFLF
                     GSEEALIQLDMSEFMERHSVSRLVGAPPGYVGYEDAGQLTEALRRRPYSIVVFDEVEK
                     AHPEAHNMLLQIMEEGHLSDARGHKVDFRNAIIVMTSNVGADMIKRQTSLGFAMKRDE
                     ETEERMAYEEMRKKLLDSLKRVFRPEFINRLDSVIVFRSLNKDDIKKIVELELNKVSQ
                     RLVEHDLHLRATPEALEMLADLGFDPEMGARPLRRVIQQKIEDRLSDALLSGEFKDGD
                     IISVEVVEGELKLDRSDVKEETPPEEAVATAS"
     gene            5221..6633
                     /gene="radA"
                     /locus_tag="AC16CB_13800"
     CDS             5221..6633
                     /gene="radA"
                     /locus_tag="AC16CB_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR3343590.1"
                     /translation="MPKSRTQYVCQQCGRVSPQYMGRCPQCQSWNSMVEEVVAPPSKS
                     KRSLVPGLVGRSQPTRLSEISGEADERLPLSIGEFARVLGGGVVPGSIVLIGGDPGIG
                     KSTLLLQVALAIAHHFSVLYVSGEESARQIKMRAARLLREAGDEGKPFPEDLYLVTET
                     NLDVILEHVTELKPRLLVVDSIQTTAMAELASSAGSVSQVRECASRLRELAKSSGIAV
                     FLIGHVTKEGAIAGPRVLEHIVDTVLYLEGDRFQSYRLLRSVKNRFGATSEVGVFEMG
                     EGGMAEVTNPSEAFLAERMVNAPGSAIAVTMEGTRPLLVEVQGLTSPTSFGNPRRTPN
                     GVDFNRLLLVAAVLTRRMGLRLGDQDVFVNVVGGLQVDEPAADLAVAAAIASSVRNLP
                     VRAETVLIGEVGLSGELRNVGQMPARLREAANLGFKSAIVPRRMRGAKPADPAGIQVV
                     EARSLKQALDMALLAEAKEK"
     gene            6749..7612
                     /locus_tag="AC16CB_13805"
     CDS             6749..7612
                     /locus_tag="AC16CB_13805"
                     /inference="COORDINATES: protein motif:HMM:NF022441.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HIR3343591.1"
                     /translation="MYKRWIFILSMMVLFSFACQAVTGIGGVVRGSGEVVSETRQVSG
                     FDQISVCCGMQLIVTQGDVESLEIEADDNLLPEIVSTVSGGRLTIRFKDDQGRTQYWP
                     SQPIRCTVTAIAVRRLEVSGGGSLDAGLIETDDLQIDTSGGSQADVASVMADTMRVEV
                     SGGGEFHAQDLQVNRLELDLSGGSTGSIDSLEGETLKLDNSGGGEVMVAGRVTEQDVS
                     ISGGGKHLAGDLKSETVAISMSGGGQAGLWVEESLDANLSGGARVEYYGRPRITEQVS
                     GGSELVSLGER"
     gene            7640..8428
                     /locus_tag="AC16CB_13810"
     CDS             7640..8428
                     /locus_tag="AC16CB_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012945370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HIR3343592.1"
                     /translation="MKSVNYQVVPYPKSRQPIVDYLTVARSKHTIHGILEFDVTAPRQ
                     VMREHKTRTGESPSFTAFIICCLAKAIDENKKLHAYRRGRNWLVIYDEVDISTPVERE
                     VKGHRIGTPYVVRSANAKTFREIHQEIRTAQVEEAGAHTRMQRSGLLPNALTRLFWML
                     LAISPHLKKKYTGTVGITAVGMFGKGAGWGIPITDYTLQVTLGGIAEKPWVVDGRIEV
                     RDILSLTLSFDHDVVDGAPAARFAQRFKELVESGYGLITDQGDG"
BASE COUNT         1917 a         2133 c         2654 g         1734 t
ORIGIN      
        1 tgcgctcctg ggcaacaata gctgtaaacg gataaatagg cggcatgtac ttcctcggat
       61 tagcattata actcaattta ttcaccctct tttgagaagg atgtttgcat atgggatcat
      121 gggttgctga tctaccctgt taagatcatt tgggcaagtt ctctatcacc tgtccagggc
      181 aattgcatat gccctgaggg gcatgcgcaa tcccgcaaaa gagtgtttgg ggtgttttgg
      241 tgggggtatg cccccaccaa aacaccctat tttctctcct cagtggtggg caatcgccca
      301 aatgcgattg ccctaatcac ctgtctcaaa aggttgcaaa ctttctttta acgctttata
      361 atgtgtgcca tatcttaatc agtatccgga gtaagcattt cttatggaat caaataacgc
      421 ccttcccggg cagggggtgg agcccgaaag caaagaatca accgaacaaa agccagaaga
      481 acgtccaaca accgatcttt caacaatggc aattctattg gaagagcaag gcctggtcga
      541 tcttccaaaa acaggtgaag tgcgctcagg tgtgatcgcc gctattggcg agaacgaaat
      601 cctggtcagc gttggcacca aatcagaagg ggtaatctca ggccgcgagc tagatggaat
      661 tcctgccgag gaacgcgctg aattcgcggt gggaacagag atccctgttt acgtggtgac
      721 gccggaagac accaatggca atgtggtgct ttcctatctg cgcgcccgtg aagagaaaga
      781 ctggcacgat gccgagcaga tgttggaaga tgaagatggg tttgaaagca agatcatcgg
      841 ttataacaaa ggtggcttat tagtgccctt gggcagattg cgtggctttg tacccgcttc
      901 gcagatcagc ctggtgcgca gagctaccct gggcggagat tcccctgacc agcgctgggg
      961 caagatggta ggtgagccga tcaaggtgcg cgtgattgaa gtagaccgcg agcgtcatcg
     1021 cctgatcctc tctgagcggg ctgccttgca ggagacccgc gagaccctaa aggatcgcct
     1081 gttggatgaa ttatcagaag gcgatgtgcg caccggtcgg gtgactagcg ttgccgactt
     1141 tggcgctttc gtgaatattg acggcgcaga tgggctggtc catctgtctg agatttcctg
     1201 ggagcggatt aatcatccca atgaagtgct caaggtgggg caggatgtgc aggtgaaggt
     1261 gatcagcgtg gatcacgagc gccggcgcat tggcctgtcc atccgccagt tgcaggaaga
     1321 cccctggctg aaaaagatca agggcatgcg agaaggccag ttggtagaag ccacggtcac
     1381 ccacctgacc aagtttggcg cttttgcccg tttggacgag gagttggaag gcttgatcca
     1441 tatctcagag ctgagcgagc agcgcatcaa ccatcccaaa gaggtagtca aggagggcga
     1501 ggtggtcacc ctgcgggtga tcaagatcga tcccgagcgg cggcgcatcg gtttgagccg
     1561 tcgcaaggtg gattcgcctg cctattcaga tctggattgg aagatggctc tcgcagaaga
     1621 agtgcaggaa gtgcaagaga ccggcgcccc ccagccgtct gctgaagacg agggacagga
     1681 gacaattgtc tcgcaggtcg ccgccgagga gatagttcag gaagctgaag ccatagctga
     1741 aacctcagtt gaggaagaaa ccgttgaagt catggctgaa gccaaggctg aagaagaaac
     1801 tgcggaagcc ggagttgaag cccaggccga agaagaggcg gctgaacctg cagttgaagc
     1861 ctcgaatgag gttgtagctg aagccaagac tgaagaagaa actgcggaag ctgaagatga
     1921 agcccaggcc gaagaagagg cggctgaacc tgaagttaaa gcctcgaatg aggttgtagc
     1981 tgaagcccaa gccgaagaag aaactgcgga agccgaagat gaagcccagg ccgaagaaga
     2041 ggcggctgaa cctgaagttg aagcctcgaa tgaggttgta gctgaagccc aggccgaaga
     2101 agaaactgcg gaagccgaag ttgaagccca ggctgaagaa aaggcggctg aacctgaagt
     2161 tgaagcctcg aatgaggtcg tagctgaagc ccaggccgaa gaagaaacgg catctaaagc
     2221 cgaataagat atcccacact ggattcaggc gcgccagcaa tggcgcgctt ttctttataa
     2281 caggggttgc acaattggga atctttgcct tcaacgatgt atctgtgtaa gattaacttc
     2341 atttcgcggg cgctgctagc tgatacctgc catgctatct aagagtttcg ttaatcttac
     2401 aatcttgatc agttaaaaaa tatccccctt gcgcaaactc tggtagaatc caattggact
     2461 aaaggtctcg taatcttgag aggaccgtct ttggtcttaa tgatcgttct ttgtttcagg
     2521 aggtaaacca ttggccggca tggagcgttt cacacagcgg gcgcgtcgtg tgctcagcct
     2581 agcccatcag gaagctgagc gaatgcgcca taattacatc ggtacggagc acttgcttct
     2641 aggcctgatt cgcgaagaag gtggtgtagc cggacgcgtg ttgcgagaat tgggtttaga
     2701 gccagaacgc gtgcaggaaa tagtagagcg cctgaccgga accgggcagt accggggggg
     2761 taagctggac ctctcccctg ggacgcagca ggtgctggaa tatgccgtag atgaagcccg
     2821 gcgaatggga catcactata tcggcacgga gcatctccta ttgggactag ttcgctatgg
     2881 cgagggagtc gcgatggacg tgctgcgcaa gctgggtgtc acgccggagc aaatccgacg
     2941 ccagacgcga cgagtgcttc aggagagcac cgcctcccgc cgcaccagtg tgggagagtc
     3001 acagaaaaca gctaagcagg agtcgcagaa gcccaagacc cccatggtcg accagcttgc
     3061 tactgacctg accggtctgg ctgaagaaga taaattggat ccggtgatcg ggcgccagat
     3121 ggagatcgaa cgcgtcatcc agatcctggc ccggcgcaca aaaaataatc cagctttgat
     3181 tggagagcct ggcgtgggta agacggccat tgtggagggg ctggctcagc gcattgtaga
     3241 gggcgatgta cccgctccct tgctgagcaa gcgtgtcttg cagctcgatg ttggctccct
     3301 ggtggcaggc accatgtatc gcggccagtt tgaggagcgc ctgaagcgcg tcatcgagga
     3361 gctcaaatcg tctggagcca tcctgttcat cgacgaggta catatgttag tgggtgcagg
     3421 cgcggcggga tcgtcggtgg atgctgccaa catcttgaag ccagcgcttt cgcgcgggga
     3481 attacaggtg attggtgcta ccacattgga tgagtaccgc aagcatatcg agagcgacgc
     3541 ggctctggag cgccgcttcc aacctattat ggtggaagaa cccagtgaag aggaaacgat
     3601 cgaaattctg cgcggaatcc gaagcgctta tgaagaacac caccgtctga ccatcagcga
     3661 tgaggccctg gatgcggcgg tgcacctctc ggcgcgttat gtgaccgagc gtttcctgcc
     3721 agataaagcc atcgatctga ttgacgaatc atcatctcga gtgcgcatgt ataaaagcga
     3781 cgctgctcag gctgcgaaag aattgatgcg cgagctacgt gagctgcgta ccaaccacgc
     3841 catcgcactg gaagataatc gctacgatga cgcccaggac tatctggatc gccaggaaaa
     3901 cctcgagatg cagctcgagc gtatgcgcac cgtgtgggac cgggccagca gcccggtggt
     3961 ttctgcagat gacatcgctg aggtggtttc catgtggacc ggcgtgccag tgatgcagat
     4021 tgccaaggaa gagtcgcagc gcctgctgga gatggaaaag gagctgggca gccatattgt
     4081 aggccaggag gaggccatcc aggccatctc caaggcggta cgtcgtgccc gcgctggttt
     4141 gaaggatccg cgccgcccga ttggttcctt tatcttcctc ggccccacgg gtgtgggcaa
     4201 gaccgagttg accaaagcta tggcccgctt cctgtttggc agcgaagaag cgctgatcca
     4261 gctcgacatg tccgagttca tggagcgcca ctcagtgagc cgcctggtgg gagccccccc
     4321 aggttatgtg ggttacgaag atgccggaca actcaccgaa gccctgcgcc gccgccccta
     4381 ctcgattgtg gtcttcgatg aggttgagaa agcccatcca gaagcgcaca atatgctgct
     4441 gcagatcatg gaggaaggac acctttccga tgctcgcggt cataaggtgg atttccgcaa
     4501 tgctatcatc gtgatgacat ctaacgtggg cgccgatatg atcaagcgcc agacgtcgct
     4561 gggttttgcc atgaaacgcg atgaggaaac cgaggagcgc atggcctatg aggagatgcg
     4621 caagaaactg ctcgattcgc tcaagcgcgt cttccgccca gagttcatca accgcctgga
     4681 ttcggtgatc gtcttccgct cgctcaacaa agatgatatc aagaagatcg ttgagctgga
     4741 gctgaacaag gtcagccagc gactggtgga gcacgacctc cacctgcgcg ccaccccaga
     4801 agccctggag atgctcgctg acctgggatt cgatccggag atgggcgcgc gacctctgcg
     4861 ccgcgtgatc cagcagaaaa tagaggatcg gctatcggat gccttgctct ccggtgagtt
     4921 caaggacggc gatatcatta gcgtggaagt ggtagagggc gagctcaagc tagatcgcag
     4981 tgacgtgaaa gaggagacgc caccggaaga ggcagtggcg acagcctctt gacccaataa
     5041 gctaatcgtg atcgtaacgt cttgcaggtg cgtaaaccct gcaagacgtt ttttctatta
     5101 ggggatcagg tacacaatca ggggattgcg cggtttcagc aaaaaggcgc tcctctaata
     5161 gatatgcgcc tgctgcggat atatgagcgc ttgtcatctg tctttgctat aatatccact
     5221 atgccaaaat caagaactca atacgtttgc cagcagtgcg ggcgcgtctc tccccagtat
     5281 atggggcgtt gcccgcagtg ccaaagctgg aacagcatgg tcgaggaagt tgtcgcaccg
     5341 ccgtccaagt cgaagcgtag ccttgttccg gggttggttg ggcgctccca gcctacacgc
     5401 ctgtccgaaa tcagcgggga ggccgacgag cgcctgcccc tctcgatcgg ggagtttgct
     5461 cgcgtgctgg gcggtggggt ggtgcccggc tcgatcgtgt tgatcggcgg cgacccgggc
     5521 attggcaagt ccaccctgct actgcaggtg gcgcttgcca tcgcgcacca cttctctgtg
     5581 ctgtatgtat ctggcgagga gtcggcgcgg cagattaaga tgcgcgccgc tcgattgctg
     5641 cgcgaggcgg gggatgaggg taagcctttc cctgaagacc tgtacctggt taccgagacc
     5701 aacctggatg tcatcctgga gcatgtcacc gaactcaagc cgcgcctgct ggtcgtagac
     5761 tcgatccaga ccactgccat ggctgagctg gcatcgtcgg cggggtcggt ttcacaggtg
     5821 cgcgagtgcg cctcccgtct gcgcgagctg gccaagtctt cgggcatcgc cgtgttccta
     5881 atcggtcacg tgaccaagga gggtgccatc gccgggccgc gcgtgctgga gcacattgtc
     5941 gatacggtgc tttacctgga gggtgaccgc ttccaatcat accgcctgct gcgcagcgtg
     6001 aagaatcgct ttggcgctac gtcggaggtg ggcgtattcg agatgggcga gggcggcatg
     6061 gctgaggtga ccaacccgtc ggaggccttc ttggcagagc gcatggtcaa cgcccctggc
     6121 tcggccattg cggtgaccat ggagggcacg cgccccctgc tggtggaggt gcagggactg
     6181 accagcccca cgtcctttgg caacccgcgg cgcacaccca acggggtgga tttcaaccgc
     6241 ctgctgctgg tagccgcggt gctgacgcgg cgtatgggtc tgcgcctggg agatcaggac
     6301 gtatttgtga atgtggtggg cggcttgcag gttgacgagc cggcggctga cctggcagtg
     6361 gcggcagcta ttgcctcttc ggtacgcaat ctgccggtgc gagcggagac ggtgctgatt
     6421 ggcgaggtgg ggttatcggg tgagctgcgt aatgtagggc agatgccagc ccgcctgcgc
     6481 gaggcggcca acctcgggtt taagtcagcg attgtgcccc ggcgcatgcg cggcgcaaag
     6541 ccagcagacc cggcgggcat ccaggtggtg gaagcgcgct ccttgaagca ggcgctggat
     6601 atggcgctgc tggcagaagc gaaggaaaag tagagcgttt gatcatatct ctgagattca
     6661 ttggctggtt taaaacatgc tctattttga tctcagttga tggggatggg tttgaagaca
     6721 aaaagtagat gtgggaaggg agtcacagat gtacaagcga tggattttta tacttagcat
     6781 gatggttttg ttttcatttg cgtgccaggc tgtcacgggt atagggggtg tcgtaagagg
     6841 ttcgggagag gtggtcagcg aaacccgcca ggtgagcggt ttcgaccaga tcagcgtgtg
     6901 ctgcggcatg caattgatcg tcacccaagg cgatgtggag tccctggaaa ttgaggccga
     6961 cgacaacctg ctgccggaga tcgtgtcgac tgtgtctgga ggaaggctga ccatccgctt
     7021 taaagatgac cagggcagga ctcaatactg gcccagccag ccgatacgct gtacggtgac
     7081 cgccatcgct gtgcgccgcc tggaggtctc gggtgggggc agcctggatg ccgggctgat
     7141 cgaaacggat gaccttcaaa tagataccag cggcggcagc caagctgatg tcgcctcagt
     7201 gatggcggat acgatgcggg ttgaggtctc aggtgggggc gagttccacg cccaggatct
     7261 acaggtcaat cgcctggagc tagatttgag cggtggcagc acaggctcca ttgattcgct
     7321 ggaaggcgaa acgctcaagc tggataacag cggtgggggt gaggtgatgg tcgctgggcg
     7381 cgtgacagag caagatgtat ccatcagtgg cggcggcaag cacctagctg gggatctaaa
     7441 aagcgagacg gtcgccatct cgatgtctgg tggcggacag gctggattgt gggtggaaga
     7501 gtccctggat gccaatctca gtgggggggc gcgggtagag tattacggca ggccacgcat
     7561 cacagagcag gtttctgggg gcagtgagct ggtcagcctg ggcgagcggt aggcagcgta
     7621 cattcacaga ggcatatcaa tgaagtcagt taactatcag gttgtaccct atccgaaatc
     7681 ccgacaaccc attgtcgatt accttaccgt cgcccgcagc aaacatacca ttcacggaat
     7741 cctcgaattt gatgtcactg cgccgcggca ggtcatgcgg gagcacaaaa cacgcaccgg
     7801 ggaatcgcca tccttcacgg cttttatcat ttgctgcctg gccaaggcga tcgatgagaa
     7861 taaaaagctg cacgcctacc ggcgggggcg gaactggctg gtcatctacg atgaggtgga
     7921 tatcagcacc cccgtcgagc gagaagtcaa aggtcaccgg attgggactc cctatgtcgt
     7981 tcggtcagca aacgcgaaaa ccttccggga gattcatcaa gagatccgca ccgcccaggt
     8041 ggaagaggcg ggtgcccata cccgcatgca gcgatctggg ctgctgccca atgccctcac
     8101 tcggctgttt tggatgctgc ttgcgatatc cccgcacttg aagaagaagt acacgggcac
     8161 ggtcggtatc accgccgtgg gtatgttcgg aaaaggtgcg ggttggggga tacccattac
     8221 cgactatacg ctgcaggtca cgttgggggg aattgcggaa aaaccttggg tggttgatgg
     8281 ccgtatcgaa gtgcgcgaca tactcagcct cacgctcagc tttgatcatg atgtcgttga
     8341 tggagcgcca gccgcgcgct tcgcgcagcg atttaaagag ctggtcgagt ctggttatgg
     8401 tctcatcact gatcagggtg atggctaaag agcgagag
//
LOCUS       DBNGSX010000469         4303 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_47208, whole genome shotgun sequence.
ACCESSION   DBNGSX010000469 DBNGSX010000000
VERSION     DBNGSX010000469.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_47208"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="AC16CB_13815"
     CDS             <1..795
                     /locus_tag="AC16CB_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding domain-containing protein"
                     /protein_id="HIR3343593.1"
                     /translation="ANTRIIGPSAGRVNLQTTTLGVMRVDIDRLNEINQFEGITLATL
                     ASHTPVTPRKTIATIKIIPFALPEEVVAAVEAIARHDSPIVSVNSLSEKCVSVIFSGS
                     PSIKDRLLKDFAPLFERIENLGSKIQQVDFIPLEDESGETALAEMLEQRIREDGNLIL
                     LAGETAIMDRYDIAPRAVERAGGQVECVGVPVDPGNLLMLGYIGPVPVLGVPGCARSK
                     KTNIVDWVLPRLLAGDRLARYDFMRLGHGGLLEDTRQRPMPRNTVF"
     gene            951..1154
                     /locus_tag="AC16CB_13820"
     CDS             951..1154
                     /locus_tag="AC16CB_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343594.1"
                     /translation="MTGDTVYTSEIRIERIKGPHRLAYLPVTPAPVHFGVHSEIAEHY
                     NIDSNVHNPYAATIDYVIAATGG"
     gene            1155..1427
                     /locus_tag="AC16CB_13825"
     CDS             1155..1427
                     /locus_tag="AC16CB_13825"
                     /inference="COORDINATES: protein motif:HMM:NF014608.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR3343595.1"
                     /translation="MTGTFGGALEARGIPAGEGYLTSQIRGEVGKDEKVLIIRRIHVA
                     YTLKIADEHRETAQRAHGFHAQYCPVARSIRDCIEISTELQMEHLS"
     gene            1570..2454
                     /locus_tag="AC16CB_13830"
     CDS             1570..2454
                     /locus_tag="AC16CB_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3343596.1"
                     /translation="MPIESIEALQEALRQKLYIAERGLATSIYLAHKLSRPLFLEGDA
                     GVGKTEIAKVMANILDTELIRLQCYEGLDVHHAVYEWNYTRQMMAIRLMEAQGEHTSE
                     DELFSNKFLLRRPLLQAIESKNGKPPVLLVDEIDRSDEEFEAFLLEVLSDFQITIPEI
                     GTIRASEPPFVVITSNRTREVHDALKRRCIYYWIDYPPFEKELQIVQMKIPQASNNLA
                     RQITQFIQELRQADLYKIPGIAETIDWTTALVALDQTALDVSIIQETLGVILKYHDDI
                     DKIGHEELKSILNRVKNA"
     gene            2447..3667
                     /locus_tag="AC16CB_13835"
     CDS             2447..3667
                     /locus_tag="AC16CB_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3343597.1"
                     /translation="MPDPNAVPDHLKKESTILYNLLLFGRLLRELGMDVNPSRMMDLI
                     HALEFINIGIRTDFYHASRGLLVHKKSDIPLFDQAFDLFWQKPMEEWFTIDLGMLTQK
                     QKPKPLIIPPPLSPPEEPPSELPDEPDDDLQTLVEVTVTYSQRETLRQKDFSELTGEE
                     LEAVKKLMENLSLQLGDRLSRRRRPGKRERLDLRRSMRKSYKHGGEILHWARLDRKTK
                     PRPIIIIADISGSMERYTRLLLHFIYGLAQDRLHPVEAFVFSTRLTRITRQLNNREIE
                     TALRQVSNTVHDWAGGTRIGESLKTFNFDWGRRVLGRGAVVMIISDGWDRGDIDLLET
                     EMDRLQRSCFRTIWLNPRLGSPEYEPLTRGIVAALPYVDDFLPVHNLASLEDLADHLA
                     KVSSGRSWHTKNLS"
     gene            3678..>4303
                     /locus_tag="AC16CB_13840"
     CDS             3678..>4303
                     /locus_tag="AC16CB_13840"
                     /inference="COORDINATES: protein motif:HMM:NF014662.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR3343598.1"
                     /translation="MYDILPKVSKWVEDGQTAAMATVIRTWGSSPRQAGAKMAVNQHG
                     EMTGSVSGGCVEGAVVEAALESLQTGQAQYLHFGVADETAWEVGLACGGMIDVFVQPL
                     DPEIFRQQRSAYETDAASAHLVQVKGPPDTIGRQWFSLLDNAASYFHDSGIPSQVADA
                     ASSAMSSRVIDASTQEGGEVEFFLDIQLPKPLLIIVGGVHISVALISMA"
BASE COUNT         1046 a         1158 c         1162 g          937 t
ORIGIN      
        1 gccaacacca ggatcattgg tccttcggct ggtcgggtga acctgcaaac aacgacctta
       61 ggcgtgatgc gtgtggatat cgaccggctc aacgagatca accaatttga agggatcacg
      121 ctggccaccc tggccagcca cacccccgtt acccccagaa aaactatcgc aacgatcaag
      181 atcatcccct ttgcacttcc agaagaggtg gtggcggctg tcgaggcaat cgctcgccat
      241 gatagcccca ttgtatccgt gaacagtctc tcagaaaaat gtgtgagcgt gattttttcc
      301 ggatcccctt cgataaaaga taggctgctc aaggactttg ctcccctgtt tgagcgcatt
      361 gagaacctgg gctcgaaaat ccaacaagtg gactttatcc cactggagga tgaaagtggg
      421 gaaaccgcgc tggctgaaat gctcgagcag cgcatccgcg aagatgggaa tttgattttg
      481 ctcgccggtg aaacggcgat catggaccgc tacgatattg cccctcgagc ggtggagcgc
      541 gccgggggac aggtcgaatg cgtgggggtc ccggtcgacc ccggcaactt gctcatgctg
      601 ggttatatcg gcccggtacc cgtcctcgga gtgccgggtt gtgcacggag taagaaaacc
      661 aatattgtag attgggtgct gcccaggctg ttggcaggag acaggctggc gcgatatgat
      721 tttatgcgtc tgggtcacgg cgggttgttg gaggataccc gccagaggcc tatgccgcgg
      781 aacactgttt tttaaccaat aaaatagaag gtacgattaa caaagatcct aagggttttc
      841 atccaatacc atgctcatta ttcgtaatga gaacccttag gatcctgttg gcaaataacg
      901 aaaacagagg ctgatcttag cttgtattca agtccagacc ataaaaatat atgacaggtg
      961 ataccgttta tacctcagaa atccgcattg agcgcatcaa gggtccccac cggcttgcct
     1021 acctgcctgt gacaccggcc ccagtccatt ttggcgtgca ttctgaaatt gcggagcact
     1081 ataacatcga ctcgaacgtg cacaacccgt acgctgcgac gatcgattat gtgatcgccg
     1141 caaccggtgg ctgactgacc gggaccttcg gcggcgcgct ggaagcgcgc gggatcccgg
     1201 caggtgaagg ttatctcacc tcacaaatca gaggggaagt aggcaaggac gagaaggtgc
     1261 tgattattcg tcgcattcac gtagcctata cgctaaaaat cgcagacgaa caccgcgaga
     1321 ctgcccagcg cgcccatggt ttccacgcac aatactgccc ggtggcgcgg tccataaggg
     1381 attgcatcga aatctcgacc gaattgcaga tggaacatct ctcgtaatcg agaccctgag
     1441 gctctaatat ccgactattc gaatcctagt tgaccgtcgt tggattgaaa tttagatcac
     1501 ctatcctaac gattactgaa ccagaaaaac agatcttgtc ctatagaaaa tatcgagtat
     1561 catcaagtca tgcccattga atcaattgaa gccttgcaag aggccctgcg ccaaaaattg
     1621 tatatcgccg aacggggtct ggcgacctcg atctacttgg cccataaact ctcccgcccg
     1681 cttttcctgg aaggtgatgc tggcgtaggc aaaactgaga ttgccaaggt catggccaac
     1741 atcctggaca ctgaacttat ccgcctgcaa tgctacgaag gcttggatgt tcaccatgct
     1801 gtgtatgagt ggaactacac ccggcagatg atggcaatcc ggctgatgga ggcccaggga
     1861 gagcacacct cggaagacga attgttcagc aataaattcc tgctgcggcg gcccttgctc
     1921 caggcgattg aaagcaaaaa cggcaagccc ccggttttgc ttgtcgatga gatcgaccgg
     1981 agcgatgaag aatttgaagc tttcctgctc gaggtgctct ccgattttca gatcactatc
     2041 cccgaaattg gcactatccg tgccagcgaa ccgccctttg tggtcattac ctccaaccgg
     2101 acacgggagg ttcacgatgc actcaaacgg cgctgcattt attattggat cgattaccct
     2161 ccctttgaaa aagagctgca gatcgttcaa atgaaaatcc cccaggcttc caacaatttg
     2221 gcccggcaga tcactcagtt tatccaggaa ctgcgccagg ctgatttata taaaatccct
     2281 gggattgctg aaacgatcga ttggaccacc gccctggtag cattagacca aaccgctctg
     2341 gatgtatcga tcatccagga gaccctgggg gtgattctta aatatcacga tgacatcgat
     2401 aaaattggac atgaggaatt gaaatcgatt ctgaatcgtg ttaagaatgc ctgacccaaa
     2461 cgctgtcccc gaccatctaa aaaaggagag cacgatactg tacaacctgc tgctcttcgg
     2521 caggctgctc cgcgaactgg ggatggatgt caatccaagc cggatgatgg acttaattca
     2581 cgccttggaa tttatcaaca tcggcattcg aaccgatttc taccatgcct cccgcggact
     2641 gctggtccat aaaaagagcg atatcccgct cttcgaccag gcattcgacc tcttttggca
     2701 aaagccgatg gaggaatggt tcactatcga cctggggatg ctcacccaaa aacagaaacc
     2761 gaagcctttg attatccccc caccacttag cccgccagag gagccgccat ccgagctgcc
     2821 agatgagcca gatgatgacc tgcaaacatt ggtggaggtc accgttactt acagccagag
     2881 ggaaaccttg cgccagaaag atttttctga gctgaccgga gaagagcttg aggcagtcaa
     2941 gaagttaatg gagaatttat ccctccagct tggggatcgg ttgagccggc ggcgaaggcc
     3001 cggaaaaaga gagcggctgg acctgcggcg atcgatgcgt aaaagctaca aacacggcgg
     3061 tgagatcctc cactgggccc ggttggatcg caagaccaaa ccccgtccta tcatcattat
     3121 cgctgatatc agcggttcga tggaacgcta cacccgcctg ctactccact tcatctacgg
     3181 cctcgcccag gaccggctcc atccagtaga agcgtttgtc tttagcaccc ggttaacccg
     3241 cattacccgg cagctcaaca accgcgagat cgaaaccgct ctgcgccagg tctcgaacac
     3301 ggtccacgat tgggctggag gcacgcgaat aggcgaatca ttgaaaacct ttaatttcga
     3361 ttgggggcgg cgggtgctgg ggcgtggtgc agtggtgatg atcatcagcg atggttggga
     3421 ccgcggcgat attgacctgc ttgagaccga gatggacaga ttgcagcgta gctgctttcg
     3481 caccatctgg ttgaatccca ggttaggatc cccagagtac gaaccgctca ccaggggcat
     3541 agtagctgcc ttgccctatg tggacgattt cctgcccgtc cacaacctgg caagcctgga
     3601 agatcttgcc gaccacctcg ccaaggtctc ttcgggcagg agctggcaca caaaaaacct
     3661 gagttgagag gcactgcatg tacgacatcc ttccaaaagt aagcaaatgg gtagaagatg
     3721 ggcagactgc tgccatggcg accgttatcc gcacctgggg ttcctcgccc aggcaggctg
     3781 gcgccaagat ggcggtcaac cagcacgggg agatgacggg ttctgtcagt ggaggctgtg
     3841 tggagggagc agtggtcgag gcggccttgg aatccctaca gacgggtcag gcacagtacc
     3901 tgcactttgg cgttgccgac gaaaccgcct gggaggttgg gctggcctgc gggggcatga
     3961 tcgacgtctt cgtgcagccg ctggatcctg agatattccg tcagcagcgc agtgcgtatg
     4021 aaacggatgc cgcctctgca catcttgtac aggtcaaggg tcccccggat actattgggc
     4081 gccagtggtt ctccctgctt gacaatgcag cctcgtattt ccatgactcg ggcattccct
     4141 cgcaggttgc agacgcggca tccagcgcaa tgtcctcgcg agtcattgac gcttccacgc
     4201 aggagggggg agaggtagag tttttccttg atatccagct ccccaagccg ttattgatca
     4261 tcgtgggcgg ggtgcacatc tcggtcgctc tgataagcat ggc
//
LOCUS       DBNGSX010000470        22238 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_116023, whole genome shotgun sequence.
ACCESSION   DBNGSX010000470 DBNGSX010000000
VERSION     DBNGSX010000470.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 22238)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 22238)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22238
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_116023"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1402)
                     /gene="hpaE"
                     /locus_tag="AC16CB_13845"
     CDS             complement(<1..1402)
                     /gene="hpaE"
                     /locus_tag="AC16CB_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HIR3343599.1"
                     /translation="MVNHFIDNKFVPGLEGGRFETLNPANNQPITEVASGSAPDADRA
                     VKAARAAFDQGPWPRLPAEERAKALRRIGDLIEKHADEIAEVEIADTGVPVSQIKKGA
                     IPRAAENFYFFAEMATQITGETYPVEGQFMTYTVRRPVGVAGLITPWNVPFMLETWKV
                     APCLAAGNTCILKPAEWAPLSADKLAHIIQEADLPPGVFNVLHGYGETAGASLVAHPG
                     VQLISFTGETSTGKEIMRNGAATLKRYSMELGGKSPTIIFDDADLERALDGAIWQVYS
                     LNGERCTAGSRLLLQKSVYDDFLARLEARVRNIRVGDPTDPRTEVGPLIHSDHWDRVH
                     GYMDVARQEGASILVGGGRPATLQQGNYFEATLIAGVSNEMRVAQEEIFGPVLVVIPF
                     DDDADALRMANDIPYGLAAYVWTNDLKRGHQIAEAIESGLVWVNSQNVRDLRTPFGGC
                     KASGIGREGGHYSFDFY"
     gene            complement(1425..2171)
                     /locus_tag="AC16CB_13850"
     CDS             complement(1425..2171)
                     /locus_tag="AC16CB_13850"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR3343600.1"
                     /translation="MRLVRFSVAGQLHSGVVDPDGNLRSSQGERYNPDEVVWLPPVEP
                     SKVIGLALNYAEHASELQMAPPDEPALFFKPISSLVGHGAPIIYPSGVDYMHYEVELA
                     VVIGKRCRKVSRQDADQVIAGYTIANDVTVRDYVGNFYRPPVRAKGYDSFGPVGPALV
                     TPNEIPDASKLELRAFVNGELRQKGNTASLIKSVPELIEFISQIMTLEEGDMIWTGTP
                     KGISHVYPGDVIRLEIDGLGVLENPVIAEE"
     gene            complement(2168..3043)
                     /locus_tag="AC16CB_13855"
     CDS             complement(2168..3043)
                     /locus_tag="AC16CB_13855"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3343601.1"
                     /translation="MTATDSSVEVNGIQLSYREWPGEKGPLICLPSLALHKGSFDAIA
                     SRLSPEYHLYALDLRGRGDSDKPAEGYGFAYHTRDVLTFAEKMGMQTFGIIGHSFGAT
                     VGVYLASIRPLSLRCIVMIDGGVDPKDEVMQAMRPLLGRLGTSYPSMDAYLNTMREIP
                     YYQPWNATLERVLREDVETLPNGSVRSKTSPQAIERDLDIHFYYSMCVHFPTMQCPSL
                     FIRPRQGLLGDKAHVLDEREAAAFVNWIPKCWRVDLPDVNHYTMVLSEEPVVVPPIRA
                     FLSTIFTEAGEESES"
     gene            complement(3051..3944)
                     /gene="dapA"
                     /locus_tag="AC16CB_13860"
     CDS             complement(3051..3944)
                     /gene="dapA"
                     /locus_tag="AC16CB_13860"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014345529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR3343602.1"
                     /translation="MNDIQASLRGSIVPLVTPFKNGELDEAALSGLIEWQIASGSHGL
                     SVTGTTGEPSALSTTERERVIQVAVEAISGRLPLLAGTGSNNLEETLRLSRFAQKAGA
                     DAILVVVPYYIRPSQEGLFGYFKTVADSVELPVILYNIPGRSAINLEIDTVSRLRETC
                     PNIIGIKEANHDFAHITRLLGRLGRDFLVYSGIELLCFPILAIGGAGYVSATGNVLPR
                     QVAQLYDLFAAGKWRQAKDMHYYLMPLNDAMFIEINPVPAKTALGMMGKISPEVRLPL
                     APMSAENIGRLRGVLAQYHLL"
     gene            complement(3954..5204)
                     /locus_tag="AC16CB_13865"
     CDS             complement(3954..5204)
                     /locus_tag="AC16CB_13865"
                     /inference="COORDINATES: protein motif:HMM:NF018485.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015112 - nitrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3343603.1"
                     /translation="MNKLQASIYPLHYAWIIVAISTLVVFAALGLARFGYSSLLPPMQ
                     ASLGMNNTQAGIMATFNLIGYLTLSVIGGALAARYGPRVVITVGLALVGVAMLFTGMA
                     NTFLSAVLWRTVTGVGSGASNVPVMALVAAWFAARRRGLGSGIAVSGSSIGLIMIGSL
                     VPQVLSAYGEDGWRISWFIFGITTLILALVGWLILRNDPKEVDQSPYGLEDPQPGPKA
                     SGTGLQWGRVYRAPAVWHLGLVYIAFGFSYIIYLTFFIKRLIAEVGYTQEAAGGLFMT
                     MGWFSMLCGLIWGTISDKIGRKNALILVYLIHATSFALFAWNGSINLTLSAILFGLTA
                     WSIPAIMAATCGDVLGPRLAPAALGFITLFFGIGQALGPSVAGAMADSASSLIPAYLL
                     ASGVALLGAFGTLFLRPPSTARNN"
     gene            complement(5216..7075)
                     /locus_tag="AC16CB_13870"
     CDS             complement(5216..7075)
                     /locus_tag="AC16CB_13870"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HIR3343604.1"
                     /translation="MAYRSTNAIINLTTGDVKITRTPEALEQAYLGGRGLNMAFLRHY
                     LRAAGKPRQINPLSPENPLIFGAGMLTGTIAPNAARFNVSARSPESGILGDANCGGFF
                     AAAMRKAGFDRLVILGKAERPTYILLEDGVVHILDASDLWGTRVVEAQEGLRSKHGPG
                     TISAVIGPSGENQVRMAAVMTGIKNAAGRGGMGAVMGSKNLKAIAARGGTAIEVAQKE
                     AIRSTRLEQQKYLKDSKIVQILGRVGTPLLYEVSNRIGAIRTRNSQDNYFEDSLNSEE
                     IEKYSDKMLACTSCVVHCRHRNTLGGEGPEYSTIGLLGANLGIAPTDQVIMLNNQVNE
                     LGLDASSVGTIIGWAMELYQRGLIDEGMTEGPLTWGDYEVARELIEKIAYRQGFGNIL
                     AESSQALRYFPPEAKDYLIAVKGLPQSDPHDVRYFKGFALGIAVASRGADHLRNRPTL
                     EVFKLPDEVRAKIYGRANDPDPTGYKDKGLIVAWSDDIYAVTDSLGICKFVTHGFNSP
                     HLLGYEHFVELVSAATSLKYTPETLRQVGRRVLDTERLINADFDLTRDDDTLPKRYFD
                     DPMPARKSKGHHVDRQQFQAMLDDYYTERGWDNQGRVPVDRQQAIDEMIAQLE"
     gene            complement(7167..7427)
                     /locus_tag="AC16CB_13875"
     CDS             complement(7167..7427)
                     /locus_tag="AC16CB_13875"
                     /inference="COORDINATES: protein motif:HMM:NF014636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR3343605.1"
                     /translation="MLIYVRLAEPYWRTVGQRDLELELEDQARVSDLLALLTKRHPAL
                     KQELVQAPPHLFIGELEVSEQSPLEEGDRLYVVWPIAGGTQG"
     gene            complement(7433..8749)
                     /locus_tag="AC16CB_13880"
     CDS             complement(7433..8749)
                     /locus_tag="AC16CB_13880"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HIR3343606.1"
                     /translation="MPIWNPEYEAMPRDELKKLQGKRLRQIVEYAYNKMPFYQRKLKA
                     AGVNPEDINSLDDLKRLPYTTKADLREEYPFGTFAVPLDQVVRIHASSGTTGKPTVGG
                     YTRKDLEIWAEVMARTVTSCGVTSKDIAHNAYGYGLFTGGLGFHLGFETVGATTIPMS
                     GGLTRRQIMLMEDFGATVLTCTPSYSLVLGEEAAEIGVNFKERMKVRIGIFGAEPWTE
                     EMRGEIEEHLGLSAYDIYGLTEVIGPGVSVECEHRTGLHIQEDHFLPEIIDPDSGESL
                     DYEEEGELVFTALTKEAMPLIRYRTRDRTVLHGDKCPCGRTIARMEKIRGRTDDMLIV
                     RGVNVFPSLVEKALLSVEGLDPYYQIVVDRAKDQLDKLEILVEANRRYFEPIDTRALD
                     QLRDAAEQELSQTLGVGAVVKLMGPHSIERSEGKAKRVVDKRDLYK"
     gene            complement(8790..9398)
                     /locus_tag="AC16CB_13885"
     CDS             complement(8790..9398)
                     /locus_tag="AC16CB_13885"
                     /inference="COORDINATES: protein motif:HMM:NF013707.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HIR3343607.1"
                     /translation="MDNGTINFLLAGVGGQGTLLASDILAEVGLHVGYDAKKSEVHGM
                     AQRGGAVVSHVRWGKRVASPLCERGTVDYFVAQEMLESLRWLEYLRPGGTVVLSRQQL
                     PPTSTVFGSDVYPNDDQVIAEVSKLAGKVLALDAIGVAEGLGNRRIANSVLLGALSSE
                     LDLDPEAWITVIESKVPQRHKEANRKAFLAGREGQWELLRNP"
     gene            complement(9435..11213)
                     /gene="iorA"
                     /locus_tag="AC16CB_13890"
     CDS             complement(9435..11213)
                     /gene="iorA"
                     /locus_tag="AC16CB_13890"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HIR3343608.1"
                     /translation="MKSLMSGNEAIARGIWESGTEVATAYPGTPSTEILQNVRLYDDI
                     YAEWSANEKVALDVAIGSAYAGRRSVAAMKHVGLNVASESLFYASYTGINAGLVIITA
                     DDPGLHSSQNEQDNRHYARFAKVPMLEPTDSQEAKDYILRAFELSEQFDTPVLVRTTT
                     RTSHAKSVVALKDRQQVEKRPYVKNAPKYVMIPGYARLRHPVVEERMVKLAQWAEQTD
                     LNTIEWGDRSLGIITSGIAYQYAREVFPQASILKLGMVWPLPSEKIREFASQVSRLIV
                     VEELDPFIEENVRQIGLQVEGKSIFPLIDELTPERVRDAAIGANLLSGNGNGYKPSVG
                     PLPRRPPVLCAGCGHRDVFWVLRKVKAAVNSDIGCYALGVLPPLDSTDTIGCMGASIG
                     VAMGMRRGGLEKDNVAVIGDSTFFHAGLPALATAVYNNTPMVLIVVDNSTTGMTGHQG
                     NPASGETLRGENISRIDIAAVSRSMGVKHVTEVDARDLDAMESAVRSAIKSGETAVVV
                     AHTVCVFVTSHNRESYTVVTEDCNGCTLCYRIGCPAIYKSDILDVKTSRPKAWIDPKQ
                     CVGCGLCYDVCARQAIEPGAEKVVMV"
     gene            complement(11268..12008)
                     /locus_tag="AC16CB_13895"
     CDS             complement(11268..12008)
                     /locus_tag="AC16CB_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3343609.1"
                     /translation="MLEVQEISTYYGNIQALKGVSIQVNQGEIVSLIGANGAGKSTLL
                     NSVSGIVPSKRGVILLDGQEITHLPAETIVSLGVSQVPERRQVFSSLTVPDNLLLGAY
                     LRLRRGEKKAVYEDREYVYTLFPILRERQRQLAGTLSGGEQQMLAIGRGLMARPKVLL
                     LDEPSLGLAPLLVKEIFQVLLRLRSEGTTILLVEQNARAALDVSNRAYVLETGRIVLQ
                     GTPKELISNQEVQKAYLGKSMNHRTPKP"
     gene            complement(12001..12849)
                     /locus_tag="AC16CB_13900"
     CDS             complement(12001..12849)
                     /locus_tag="AC16CB_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019017637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3343610.1"
                     /translation="MEKKRTDTNLLSIKELRKWFGGLMAVNRISFDVKHGEIVALIGP
                     NGAGKTTIFNLITGFHPIDDGEIRFNEKRIDGLNPHKIVHEGIVRTFQNLQVFNHMSV
                     LENVKVGLHLQGHSGMITSALRMPSARQEEREFHQKALHYLEQVGLVERANDLARNLP
                     FGQQRLLEIARALAVQPQLLLLDEPAAGLTVPETNNLDELICRLQENGLTILLVEHDM
                     GLVMGIADRVVVLHYGTKIAEGTPEEVQSNSEVIRAYLGEDWQQEQWSEWQPSTPEPN
                     TEVGNA"
     gene            complement(12862..13977)
                     /locus_tag="AC16CB_13905"
     CDS             complement(12862..13977)
                     /locus_tag="AC16CB_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3343611.1"
                     /translation="MEPTAKSAPNQNLLGWLLLTVVVGVLGWVELASPFGLDFSDITR
                     GMVTLDTMIRVAILTIVLVGLNLLMGYAGQVSLGHAAFYGMGAYASAIFTARAGSLFG
                     APESLINSWWWPWLVMVLGMIFAGILAYLIGRPILRLRGHYLAMATLGLGVMIYILFR
                     ENMGFQGQHITGTFDGIPGIPRLRLGSFEIWPISRYYFFVWLVALLVIAFGLNLINSR
                     TGRALRAIHGSEVAAQAMGVDVSAYKARIFALSAILASLAGSLYAHFQASVSPAPFSF
                     IGSLELVVMSAVGGLSSIWGAPFGVAVILITKEILRGQLRLLMEGASGEHEIIFYGLI
                     LVVIMIFMPEGVTVGTVRRWRAWRNRSYEKQAQAAQS"
     gene            complement(13977..15005)
                     /locus_tag="AC16CB_13910"
     CDS             complement(13977..15005)
                     /locus_tag="AC16CB_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3343612.1"
                     /translation="MEQQSRRKLVQTIIFLGIFLALILLTVFLGGRKNFGLQQYAQLT
                     IDGLRGGAIYALIAMGFVMVFNVTGIINFAQGGFVMLGAMLAVTIYEWELPFPAWMHL
                     LISVLLAILLTSLIGILVERLSIHPARSSSTLTLIIITVGIYIATQGLALLTWGADAY
                     IYPAFTTLEMADRTIRWMGIVIKSQSFWIWGITALVLIALAFFFERTLIGKAMRACAV
                     NRRGARLVGISTSRMSVVAFALAAALGAIGGIVVAPILRPSYDMGLSLGLKGFVAAIL
                     GGLVSTPGAVLGGLMLGLIENLAAGVTKAGMKDIYAFILLILALLFRPQGLLGVRKTA
                     VEADGEDH"
     gene            complement(15068..16408)
                     /locus_tag="AC16CB_13915"
     CDS             complement(15068..16408)
                     /locus_tag="AC16CB_13915"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3343613.1"
                     /translation="MKGKLNTLFALLAIVALLLASCAQATPEPTEEPPPPPPTSAPEP
                     TEPPPPTEAPAMTEEPTPEPTEEVVGETYKIGFAPAITGPGSSLGAPEHETGQMIAEQ
                     LNAQGGVVGPDGVRHPVEIVIFDTETNPDVAVSVVRRMIEEENVQVVVAGTTSGISMA
                     IVPIITEAEVPYLSMASSGAIVKDPETGEVRKWAFKTAQDNLASAQWQALYLNKLGYT
                     KVCHLSENSGYGGDTLAQAQKAFEPAGIEIVYSDSFERTGTEFPQVINVQTSDCQAVV
                     VGAIPPASSNISIALRDALPDMPIIHGHGTCNQDHIDLMGAEVAEGMVSPCGRVMVSE
                     TLPDDDPQKDVLVQYVTDYTAYTGKPVNTFGGHGWDAVMLAVTAVSSLPDGLDLAAQR
                     AAVRDFLETGVKDWPGISGVYNLSPEDHFSLGFESLTFVKVEGGKWIYFPEEEW"
     gene            complement(16618..17925)
                     /locus_tag="AC16CB_13920"
     CDS             complement(16618..17925)
                     /locus_tag="AC16CB_13920"
                     /inference="COORDINATES: protein motif:HMM:NF012885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LeuA family protein"
                     /protein_id="HIR3343614.1"
                     /translation="MSGTTNDDVSQLIYDWNEIEEFQPPKRRILVTDETLRDGLQSPS
                     ITHPEIQDKVYLLYLMRDLGIDAADLGLCGAGERFKYDVIVLAKEIANQNMPIQPQSA
                     ARTMLDDITPIVDASQEAGIPIEACIFLGTSPIRQYAEGWDVEFLLERTKESVSFATN
                     HGLPVMFVTEDTTRAHPDTLRRVYTAAIEAGAQRLCVSDTVGHATPEGVRALVSYLRK
                     IVDEINPEVGIDWHGHRDRGMDIINTLVAIEAGADRVHACGLGVGERSGNTPMELLLV
                     NLNLMGLATRDLRRLPEYCNIIAEKCGAPVPFNYPVVGADSFRTSTGVHAAAVVKALS
                     RDENWLADRVYCGVPASMVGRVQGIEIGPMSGEHNVRFYLHQRGIEEHPVYVEKILAA
                     AKRSKRLLSEEDVLHMVAVMRERLKSGAEIKESEIEDTLLTRS"
     gene            complement(17978..18604)
                     /locus_tag="AC16CB_13925"
     CDS             complement(17978..18604)
                     /locus_tag="AC16CB_13925"
                     /inference="COORDINATES: protein motif:HMM:NF013707.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HIR3343615.1"
                     /translation="MSKVYSLIFAGVGGQGSLLIADATSLAAVQAGYDAKQTEVHGVS
                     QRGGSVETHVRFAEKVYSPLVTAGQADVVVGLEKLEALRFGHFANPEYGVILANDFEI
                     VPASVQGAEQLYPHETVDYLKNKSLRVITMPASAIARDLGDGRMANVVILGGLSMLLP
                     IPLDIWDKTLHLRIPKRYLSANLQAFEIGRNQIDIAEGFVSTNTEQKV"
     gene            complement(18619..20367)
                     /gene="iorA"
                     /locus_tag="AC16CB_13930"
     CDS             complement(18619..20367)
                     /gene="iorA"
                     /locus_tag="AC16CB_13930"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HIR3343616.1"
                     /translation="MRELLLGNQAIARGAWEAGVTFAAGYPGTPSTEIIETLATRFTE
                     TNPRWSANEKVAFEEGMGAAIGGLRVLVTMKHVGLNVAADAFMVFPYAGTNGGFVVVS
                     ADDPGMHSSQNEQDNRYLARMGKVPVLEPSDAQECKDFVIAGLELSEKYGIPVMLRTT
                     TRLAHHKGLVEIGERKDVPKRDFVPDAKRFSVPIYRRLHRPKVEERLQALKEYAETSP
                     LHRIEHTENPVGVVTSGIAYQYVKEVMPEAGVLRLGMTFPLPENLLRDFCSRYEKVYI
                     VEEGEPFIEEFMRSLGITNLVGKELFGVIGEYSPGRLMEALGMGKTPEPFTEGITLLP
                     RPPMLCIGCGHRTVFASLRQLKVTVAGDIGCYTMGALPPYEAEHTTFCMGASIGTAVG
                     LERAGYKRVVAVIGDSTFVHAGIPPLIDAVYNGSNLNLIILDNSATGMTGSQPHPASG
                     QDAQGRPAPKLNLVEVVRAAGVEHVKVVDTWQRKEVLRSIQQALANDGPSVVIAQGPC
                     QQLPEMKRREVIPYVVEEELCTQCEACFKVWCPAIKRNENNFPYIVANECTACTVCAQ
                     VCPTDAIHLADVQVTA"
     gene            complement(20408..21298)
                     /locus_tag="AC16CB_13935"
     CDS             complement(20408..21298)
                     /locus_tag="AC16CB_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3343617.1"
                     /translation="MIIDFHIHLSRPEHELPWVMEWIRSNFSGDIDAFVEEVLTPSGL
                     RRTLQANHIDWAVGLAEVSPVTTGWASNEYVGELCMQTNALPDPPSGPRGRLIPFASI
                     NPFIVNDLGAELTRLVDEYGFGGVKVYPTYHHHYPNDARLSPLYARAQELGLPVLVHT
                     GSSVFKGTRIKYGDPLLLDDVANDFPNLKILMAHSGRPFWYEQAFWMARQHSNVYMEV
                     SGLPGKKLLEYFPRLEEITDKVVYGSDWPGNPDFRRNVEAIMSLPITEQAKQDILFNN
                     AAKILGVSEREGHSTSERPS"
     gene            complement(21295..>22238)
                     /locus_tag="AC16CB_13940"
     CDS             complement(21295..>22238)
                     /locus_tag="AC16CB_13940"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR3343618.1"
                     /translation="VLVKIAACGLCHTDLHYLDHGVPTFKKPPLVLGHEVSGTIAGLG
                     AGVEQWKEGDRVLLPAVYGCGECAMCREGRENICERMVMFGNNVDGGYAEYMLAPAKD
                     ALRLPEEVPLVEGAIIADAITTPYHAVVNRGQVKPGDYVVVFGCGGIGLNVIQVAAAL
                     GAQVIAVDIIDTKLEWAKQLGAQVALNAQNFERIDKEIRKLTGGGADKGFEAIGNPVV
                     QAQTFASLRTGGRFVVVGFSAKPMTLDTGRVMYREMEIIGSLGCRAVDYPRVIDLVRQ
                     GKIKVKELVTARYPLEEINEAFDGLRRGEGIRSVVVP"
BASE COUNT         4838 a         6362 c         5973 g         5065 t
ORIGIN      
        1 tgtagaagtc gaagctgtaa tgcccgcctt cccggccgat tccactggct ttgcagccgc
       61 caaatggcgt gcgcagatcg cgcacattct gagagttcac ccacactaac cccgactcaa
      121 tagcttcagc gatttggtgt cctcgcttca ggtcgtttgt ccacacataa gctgccagcc
      181 cataagggat atcattagcc atccgcagtg catcggcgtc atcatcaaag gggatcacca
      241 ccagcaccgg gccaaaaatt tcctcctgag cgacccgcat ctcgttgctc accccggcga
      301 tcagggtagc ttcgaaatag ttgccctgct gaagagtggc tggtctgcct ccgcctacca
      361 ggatactcgc cccctcctgg cgagctacat ccatatagcc atgcaccctg tcccagtgat
      421 cagaatgtat caaaggacca acctcggtgc gcgggtcggt tggatcaccc acgcgaatat
      481 tgcgcacgcg cgcttccagg cgggcgagaa agtcatcgta gaccgatttt tgcaagagca
      541 agcgagaacc cgccgtacaa cgctcgccgt tcagggaata cacctgccag atagccccat
      601 ccagagcccg ttccaggtca gcatcatcaa agatgatagt tggcgatttt ccacccagct
      661 ccatcgagta tcgtttcaat gtggcggcgc cattgcgcat gatctctttt ccagtgctgg
      721 tttcaccggt gaaggagata agctgtaccc cagggtgggc taccagcgac gcgccggcgg
      781 tctctccata accgtgaaga acattaaaga cgcccggggg caggtctgct tcctggatga
      841 tgtgagccag tttgtccgca gagagcggcg cccattcagc gggcttgagg atgcaggtgt
      901 tgccagcagc caggcaaggc gcaaccttcc aggtttccag cataaaaggc acattccagg
      961 gcgtgatcag gccagcgaca ccaaccgggc gacggacagt gtaggtcatg aactggcctt
     1021 ccaccgggta ggtctcgcct gtgatctggg ttgccatctc agcaaaaaag tagaaatttt
     1081 ccgccgcccg cgggattgcc cctttcttga tctggctcac gggcacgccg gtatcggcaa
     1141 tctccacttc ggcaatctca tcggcgtgct tctcaattaa gtcaccaatc ctgcgcagtg
     1201 cttttgccct ctcttcagcc ggcagacgcg gccaagggcc ttgatcgaag gcggcgcgcg
     1261 ctgccttaac cgcccggtcg gcgtctggcg cgctgccaga agccacctcc gtaatgggct
     1321 ggttattggc tgggttgagc gtctcgaatc tgcccccttc aagcccaggt acaaatttgt
     1381 tatcgataaa gtgatttacc actaaaaaag cctcctagca ttggctattc ttcagcaatg
     1441 acgggatttt caagcacacc caagccatca atctccaagc gaatcacatc gcctggatag
     1501 acatgcgaaa tccctttggg tgtgccggtc cagatcatgt ctccctcttc gagggtcata
     1561 atctgagaga taaattcaat caactctgga actgacttga tcaggctggc tgtattgccc
     1621 ttctggcgca gctccccgtt gacgaaagcg cgcagctcca gcttgctggc atcagggatc
     1681 tcgttcgggg tgactaacgc tggaccaact ggtccaaacg agtcataacc cttggcgcgg
     1741 accggcgggc ggtagaaatt gccgacgtaa tctcgcacgg ttacatcgtt agcgatggta
     1801 tagccggcga tgacctgatc tgcatcctgc ctggagacct tgcggcagcg cttgccgatt
     1861 acaaccgcca attccacttc gtaatgcata taatcaaccc cgcttggata gataatgggt
     1921 gcgccatgcc ctaccagtga actgattggc ttaaaaaata aggctggctc atcgggtggt
     1981 gccatttgca gctcgctggc atgttcggca taattgagcg ccagcccgat cactttgctg
     2041 ggttcaacgg gcggcagcca gaccacctcg tcagggttgt atcgttcacc ctggcttgag
     2101 cgcagattgc catctgggtc gacgaccccg ctatggagct gaccagcaac gctaaagcgc
     2161 accagcctca tgattcggac tcctcgcccg cctcggtgaa aatcgtgctc aaaaaagccc
     2221 ggatgggtgg gacgaccacc ggctcctcgc tcagcaccat ggtgtaatgg ttgacatctg
     2281 gcagatcgac gcgccagcat ttaggaatcc agttgacaaa ggccgccgcc tcgcgctcgt
     2341 ccaggacgtg tgctttgtca cccagcagcc cctggcgcgg gcggatgaac aagctggggc
     2401 attgcatagt cgggaaatgt acacacatgc tgtaataaaa gtggatgtcc aaatcgcgct
     2461 cgatggcttg tggcgacgtt ttggagcgca cagagccatt gggcagcgtc tccacatctt
     2521 ctcgcagcac ccgttccagc gtggcattcc atggctgata gtaggggatt tctctcatag
     2581 tgttgagata tgcgtccata gatggatagc tggtacccag acggcccagc agggggcgca
     2641 tggcttgcat cacttcatct ttgggatcga ctcctccgtc gatcatcacg atgcagcgca
     2701 agctcaatgg gcggatagag gccaggtaaa cgccaaccgt agccccaaag gaatgtccga
     2761 taatgccaaa agtctgcatg cccatttttt cggcaaaagt cagtacgtcg cgggtgtggt
     2821 aggcaaatcc atagccctca gcgggtttgt cgctgtctcc gcggccgcgc aggtctaagg
     2881 catacaggtg gtattcaggc gacaggcggc ttgcaatggc atcaaaagag cccttatgca
     2941 aggcaaggct agggaggcaa atcaagggtc ctttttcacc gggccattct cgatagctca
     3001 gttgtatacc gtttacctct acgctggaat cggttgcagt catggattac ctataataaa
     3061 tgatactggg cgagcacgcc tcgcagacgc ccaatgttct cggcgctcat tggcgccagc
     3121 ggcaggcgga cttcagggct gatcttgccc atcatgccca gggcagtttt ggcgggaacc
     3181 gggttgatct cgatgaacat cgcatcgttc aggggcatca aataatagtg catgtctttg
     3241 gcttgacgcc atttgccggc ggcaaacaag tcgtatagct gggctacctg gcgaggcaat
     3301 acgttgccgg tggcgctcac atagcctgca ccgccaattg ccagtatagg aaaacacagc
     3361 aattcgatgc cggagtacac caggaaatcc cgtcccaggc gaccgagcag gcgagtgata
     3421 tgcgcgaagt cgtggttggc ttccttgatc ccgatgatgt tggggcaggt ttctcgcagc
     3481 cggctgacag tgtcgatctc caagttaatc gctgacctgc ctgggatatt gtacagaata
     3541 accggcagtt ccacagaatc ggcgaccgtc ttgaagtagc caaacagccc ttcctggctg
     3601 gggcggatgt agtagggcac aaccactagg atggcatctg cccccgcttt ttgggcaaaa
     3661 cgtgagagac gcagggtctc ttccaagttg tttgagccgg tgcctgccag gaggggcaag
     3721 cgcccagata ttgcttcgac cgctacctgg atcacacgct cgcgttccgt ggtgctcaag
     3781 gcgctgggtt cgccagtcgt gcctgtcacc gagagaccgt ggctgccgct ggcgatctgc
     3841 cattcgatca atccggataa ggcagcttca tccagctccc cattcttgaa gggggtcacc
     3901 agggggacaa tcgagcctcg cagggatgcc tgaatatcat tcatgctgtg ccttcaatta
     3961 ttcctcgcgg tcgaaggcgg gcgcagaaag agtgtgccaa aggcacccag cagcgccact
     4021 ccgcttgcca agagataggc tgggatgagc gaggacgcgg aatcggccat cgcgccggct
     4081 acactgggtc ccagcgcctg accgataccg aaaaacaggg tgatgaaacc cagggctgcc
     4141 ggggccaacc gcggtcccag gacatcaccg caagtggctg ccataattgc gggaatgctc
     4201 caggcggtca ggccaaacag gatcgctgaa agtgtgaggt tgatagagcc attccaggca
     4261 aataacgcga acgaagtcgc atggatcagg taaaccagga tcaatgcatt tttacgcccg
     4321 atcttgtccg agatggttcc ccagatgaga ccacacagca tgctgaacca gcccatggtc
     4381 atgaacaggc ctccggcggc ctcctgggta tagccgacct cggcgatcag gcgcttgata
     4441 aaaaaggtca aatagataat gtaggaaaag ccgaatgcga tgtaaaccag gcctaaatgc
     4501 cagaccgctg gagctcggta aacgcgcccc cattgcagcc cagtgcctga tgcttttgga
     4561 cctggttgcg gatcttcaag cccataaggg gactggtcaa cctccttcgg gtcatttcgc
     4621 aggattagcc accccacaag agccaggatc agcgtggtga taccaaagat gaaccagctt
     4681 attcgccagc catcctctcc gtatgctgag agaacctgtg ggacaagcga gccaatcatg
     4741 atcaggccga tggacgaacc ggagactgcg atgccggaac ctagacccct gcgccgggcg
     4801 gcaaaccagg ccgcgaccag cgccatgacc ggcacgttgc ttgccccgct gcccaccccc
     4861 gtgactgtcc tccacagcac agccgagaga aatgtgttag ccatcccagt gaagagcatt
     4921 gccacgccta ccagggcaag ccctacagtg ataaccaccc tcggaccgta gcgcgcggcc
     4981 agcgcaccgc cgatcacaga tagcgtcagg tatccaatga ggttaaaggt cgccatgatg
     5041 ccagcctggg tgttgttcat tcccagggag gcttgcatgg gcggcaataa ggaggaatag
     5101 ccaaagcgcg ccagtccaag ggcggcgaag accaccaggg tgctgatcgc cacgatgatc
     5161 caggcgtaat gcaagggata gattgaggct tgcagcttgt tcatgcgcct acttatcact
     5221 ccaattgagc aatcatttcg tcgattgctt gttgcctatc cacgggaacc cgcccctggt
     5281 tatcccagcc gcgctctgtg tagtagtcgt ccagcatggc ctgaaattgc tgacggtcca
     5341 cgtggtgtcc tttggacttg cgagcgggca tagggtcatc gaaatagcgc ttgggcaggg
     5401 tgtcatcgtc tcgggttaaa tcaaagtctg cgttgataag gcgctctgta tccagcaccc
     5461 tgcgcccaac ctggcgtaat gtttcggggg tatatttcag actagtagca gcagaaacca
     5521 attcgacaaa atgctcatag cccagcaggt gggggctgtt aaacccgtgg gtgacgaatt
     5581 tgcaaatgcc aaggctatcg gtgacggcgt agatgtcgtc gctccaggca acgataagcc
     5641 ccttgtcttt gtaccctgtg gggtcgggat cgttggcgcg gccgtaaatc ttggcccgca
     5701 cttcatcggg aagcttgaag acttccaatg tggggcgatt tcttagatga tctgcgcctc
     5761 ggctggcaac ggcaattccc aatgcaaatc ctttgaaata gcgcacgtca tgcgggtccg
     5821 attggggcag acctttgact gcgatcaaat aatccttggc ctcggggggg aaatagcgca
     5881 gcgcctggct gctttcagcc aggatattgc cgaaaccttg ccggtatgcg atcttctcaa
     5941 tcagctcacg tgcgacctcg taatcgcccc aggtcaatgg tccttcagtc atcccctcgt
     6001 cgatcaagcc gcgctggtaa agttccatcg cccagccaat gatcgtgccg accgaagaag
     6061 catccaaacc aagctcgtta acctggttgt taagcatgat gacctgatcg gtgggggcaa
     6121 tacccaggtt ggcacccagc aaaccgatgg ttgaatattc ggggccttcg ccgccaagtg
     6181 tgttgcggtg tcggcagtgg accacacaag acgtgcaagc cagcattttg tcggagtact
     6241 tttcgatctc ctctgaattc aggctgtctt caaagtaatt atcctgactg ttgcgcgtgc
     6301 ggattgcgcc tatgcggttg ctgacctcgt agaggagggg ggtgcctact cgccccaaga
     6361 tttgcacgat cttggaatcc ttgaggtatt tttgttgctc caggcgagtc gatctgatgg
     6421 cttctttctg tgccacctcg atcgctgtgc cgccacgggc tgcgatagct ttcagattct
     6481 tgctgcccat cacggcgccc atcccgcctc gcccagcggc gttcttgatt ccggtcatca
     6541 cagcagccat gcgcacctgg ttttcaccag agggtccgat gacggcagag atcgttccgg
     6601 gtccgtgctt ggacctcaag ccttcttgcg cttcgactac ccgcgtgccc cataggtcgc
     6661 tggcatccag aatgtgcacg actccatctt ccaggagaat gtaggtaggg cgctcggctt
     6721 tccctaagat caccaggcgg tcaaatccgg ctttgcgcat tgccgccgca aaaaaaccgc
     6781 cgcaattagc atctcccaat atgccgcttt ccggcgagcg ggcagatacg ttgaagcgcg
     6841 cggcattcgg ggcgatcgtg ccagtcagca tgcctgcacc gaagataagc gggttctctg
     6901 gactgagcgg gtttatctgg cgcggcttgc ctgccgcccg caagtagtgg cgcagaaatg
     6961 ccatattcaa gcctctcccg cctaggtaag cttgttctag cgcttctggg gtgcgggtga
     7021 ttttcacatc acccgtagtc aggttgatga tcgcattggt ggaacgataa gccacaatac
     7081 ctcctaaaga taccggattt gggacggtct cacctcattg cagagatgca gcgcagcatg
     7141 agcgtaaact ggctttgttt atatacctat ccttgcgtcc ctccggcgat gggccaaacc
     7201 acatataaac gatcgccttc ttccaaaggg gattgctcgc tgacctccag ctcgccgatg
     7261 aacaggtgag gcggtgcctg cacaagttct tgcttaagag caggatggcg cttggtgagc
     7321 agggccagga gatctgaaac gcgggcttga tcttcgagtt ccagctccag atctcgctgg
     7381 cccacagttc gccagtatgg ttcggcgagg cgcacgtaga tcagcataac ggttacttgt
     7441 acaagtcgcg cttgtcaacc acgcgcttgg ccttgccctc agaacgctca atcgagtgtg
     7501 ggcccatcag cttgacaact gcgccaaccc ccagggtctg agagagctct tgctcggcgg
     7561 cgtcgcgcaa ttgatccagg gcgcgggtgt cgatcggttc gaaatagcgg cgattggcct
     7621 ccaccaaaat ttccagctta tcgagctggt ccttggcgcg gtcgaccacg atctggtaat
     7681 aaggatccaa gccttctaca ctcagcaggg ctttctccac cagggatgga aatacattta
     7741 cgccgcgcac gatgagcatg tcatccgtgc ggccgcgaat cttttccatg cgggcgatgg
     7801 tgcgcccgca tgggcactta tccccgtgca gtaccgtgcg gtcgcgggtg cgatagcgga
     7861 tcaatggcat cgcctctttg gtcagcgcag tgaataccaa ctcgccttct tcctcatagt
     7921 cgaggctctc accgctatcg gggtcgatga tctcaggtaa gaagtgatct tcctggatat
     7981 gcaacccggt gcggtgctcg cattccacgg atacccccgg tccgatcact tcggttaatc
     8041 cgtagatgtc ataggcgctt aatcccaggt gctcctcgat ttcgccgcgc atctcttcgg
     8101 tccagggctc cgcgccgaag atgccaatgc gcaccttcat gcgttccttg aagttcaccc
     8161 caatttcggc cgcttcttcc cccaatacga gcgagtagga gggcgtgcag gtgagcacgg
     8221 tggcaccgaa gtcctccatc aacataattt gccgccgggt gagcccaccc gacataggga
     8281 tggttgtggc gccaactgtt tcgaacccca ggtggaagcc caacccacca gtgaatagcc
     8341 cgtaaccgta ggcattgtgg gcgatgtcct tggatgtaac cccacaggat gtgaccgtac
     8401 gcgccatcac ttctgcccaa atttctaagt ctttgcgcgt gtagccgccc acggtgggtt
     8461 tgcccgttgt accactcgag gcgtggatgc gcaccacttg atccaggggc actgcaaatg
     8521 tcccaaaggg atattcctcg cgcaggtctg cctttgtcgt gtacggaagg cgcttcagat
     8581 cgtcgaggct gttgatatcc tcaggattca cgccagccgc cttgagtttg cgttggtaga
     8641 aaggcatttt attgtaggca tattccacga tctgccgcaa tcgctttccc tggagtttct
     8701 tcagctcatc gcgcggcatc gcttcatatt cgggattcca gataggcatc gattcctcct
     8761 aagataagaa atgtttttta tacacgcttt tatgggttgc gcagtagctc ccattggcct
     8821 tctcggccgg ctaaaaatgc tttgcgattc gcctccttgt gtctttgggg gactttggat
     8881 tcgatcacag taatccatgc ttctggatca agatccagct cggatgaaag cgcgcccagc
     8941 aaaacgctgt tggcgatgcg gcggtttccc agcccctcgg cgacaccgat cgcatccaga
     9001 gcgagaacct tgccagccaa tttgctcact tctgcaatca cctggtcatc atttggatac
     9061 acatcgctgc cgaatacggt cgaggtggga ggcaattgct gcctgctgag gacaaccgtg
     9121 cctcctggcc tgagatattc cagccaacgc agagattcca gcatctcttg agccacaaaa
     9181 taatccaccg ttccacgctc gcagagaggc gaagccacgc gttttcccca gcgcacatga
     9241 cttactacag ctccgccgcg ttgcgccata ccgtggacct ccgatttttt ggcatcatag
     9301 ccaacgtgga gacctacttc agccaggata tcgctggcca acagcgttcc ctgcccaccc
     9361 acacctgcaa gcaaaaagtt aattgttccg ttatccataa catctctctc ctatctagcc
     9421 ggggtaggct gatctcaaac cataacaact ttttcagcgc cgggttcaat cgcctggcgg
     9481 gcgcatacat cgtagcacag cccgcaaccc acacactgct ttggatcaat ccaggctttg
     9541 gggcggctgg tttttacgtc caggatgtcg cttttgtaaa ttgccggaca accaatgcgg
     9601 tagcacagcg tgcaaccgtt gcaatcttcg gtgaccactg tgtaggattc gcggttgtgg
     9661 ctggttacaa acacgcacac ggtatgagcg actaccactg cagtttcgcc tgatttgatg
     9721 gcggacctca cggcggattc cattgcgtcc agatcgcggg cgtcgacttc agtgacgtgc
     9781 ttcacgccca tcgatcgaga caccgcggcg atatcgatgc gggagatatt ctcaccccgc
     9841 agtgtctctc ctgaagcagg gttgccctgg tgaccggtca tcccggtggt gctgttatcc
     9901 accacaataa gcaccatggg agtgttgttg tataccgctg tcgcaagggc ggggagacct
     9961 gcgtggaaga aagtgctgtc gccaatcacg gctacattgt ctttttcaag accgccacgg
    10021 cgcataccca tcgccacccc gatactggct cccatgcagc cgatcgtatc ggtgctatcc
    10081 agaggtggca gaacacccag ggcatagcag ccgatgtcgg aatttacggc tgcttttact
    10141 ttgcgtaata cccagaacac atctcggtga ccacagccag cgcacagtac gggggggcgg
    10201 cgaggcagcg gtcccaccga tggtttgtag ccgttgccat tcccgctcaa cagattcgca
    10261 ccaatggcgg catcgcgcac ccgctcaggt gttagttcat cgattaatgg gaagatgctt
    10321 tttccttcca cctgaagccc aatttgcctg acattttctt caatgaaagg gtctaactcc
    10381 tccaccacga taaggcggga aacctggctg gcgaactctc ggattttctc ggatgggagg
    10441 ggccagacca taccaagctt taggatcgag gcttgcggaa aaacttctcg ggcatactgg
    10501 taagcaattc cggaagtgat aatgcccaaa gagcgatcgc cccattcaat cgtgttgagg
    10561 tcggtctgtt ctgcccattg agccagtttt accatgcgct cttctaccac gggatgtcgt
    10621 agacgtgcat agccagggat catcacgtac tttggcgcgt tcttgacata aggccgtttt
    10681 tcaacctgct ggcggtcctt cagcgcaacc accgatttgg catgtgaggt gcgtgtggtg
    10741 gtacgcacca gcacgggggt gtcaaattgc tcactgagct caaacgcccg aaggatataa
    10801 tctttcgcct cctggctgtc ggtaggctcc agcatgggta ccttggcgaa gcgtgcgtaa
    10861 tggcgattgt cctgctcgtt ttgcgatgaa tgcagcccgg ggtcgtcagc ggtaatgatc
    10921 accaatcctg cattgattcc ggtgtatgag gcgtagaaga gtgactcaga agccacgttc
    10981 agcccgacgt gtttcatcgc ggcgacactc cgccgcccgg catacgcaga gccaatcgct
    11041 acatccaggg caactttctc gtttgcagac cactcagcgt aaatgtcatc atataatctt
    11101 acattttgca gtatctctgt gctgggtgtt ccgggatagg cggtggctac ctcggtgccc
    11161 gattcccaga tgcctcgggc aattgcctcg ttaccgctca ttaaggattt cattattcac
    11221 ctcctcgtaa ggttgatacc aatactttat atatatatat ggacgggtca tggttttggg
    11281 gtccggtgat tcatgctttt gcccaaatat gctttttgca cctcttgatt agagatcaat
    11341 tctttggggg tgccctgtaa gacgatgcgt cctgtctcca ggacatatgc cctgttggag
    11401 acatctagtg cagcccgggc attctgctcg accaataaga tcgttgtccc ttcagagcgc
    11461 aagcggagca gtacctggaa tatctccttg accagcagag gagccagacc cagggagggc
    11521 tcatccagga gtaagacctt aggccgcgcc atcaacccgc gaccgatggc aagcatttgc
    11581 tgctcgccac ccgagagggt tccggctaat tggcgttggc gctctcgcag gatggggaac
    11641 aaggtataca cgtactccct gtcttcgtaa actgcttttt tctcgccacg ccgcaagcgc
    11701 agatacgcgc ccaacaacaa gttatctggt acagtgagcg agctaaacac ctggcgtctt
    11761 tcgggcactt ggctgacgcc caggcttacg atggtctcgg cgggcaggtg ggtgatttct
    11821 tgtccatcca gcaggatgac gcctctcttt gaggggacga tgccagagac actattcagt
    11881 agcgtggatt ttccagcccc atttgcaccg atcaaggata cgatttcacc ctgattcacc
    11941 tgaatagaga cacccttgag cgcctggatg ttgccgtaat acgttgagat ctcctgaact
    12001 tcaagcattc ccaacctcag tatttggctc cggtgtggat ggctgccatt ctgaccattg
    12061 ctcctgctgc cagtcttctc caaggtatgc ccgtatgact tcggagttgg attgaacctc
    12121 ttctggcgtg ccctcagcga ttttggtgcc gtaatgcagc acgaccaccc ggtcggcgat
    12181 ccccattacc agacccatat catgctcaac caacaggatg gtcagcccat tttcctggag
    12241 tcggcaaatg agctcatcca ggttgtttgt ctccgggacg gtcaacccgg cggcgggctc
    12301 gtccaataga agtaattggg gctggaccgc cagggcgcgg gcgatctcca acagacgttg
    12361 ctgtccaaaa ggcaggttgc gagccaggtc gtttgcgcgc tcaaccagac cgacctgctc
    12421 cagataatgg agtgcttttt gatgaaattc acgctcttcc tgccgcgctg aaggcatgcg
    12481 caaagctgat gtgatcattc cggaatgccc ctgaagatgc aagcctacct ttacattttc
    12541 aagcaccgac atatgattga aaacctgaag gttttggaag gtgcgcacaa tgccctcatg
    12601 aacgatcttg tgggggttta agccatcgat gcgcttctca ttgaaccgaa tttctccatc
    12661 gtcgatcgga tgaaaaccgg tgatcaggtt aaatatagtg gtcttgcccg cgccattcgg
    12721 cccgattagt gcgacaattt caccgtgttt gacatcaaaa gatatccggt tgactgccat
    12781 cagcccccca aaccattttc tcaattcttt gattgaaagc aggttcgtat cggttcgctt
    12841 tttttccata aatataccaa cctatgattg cgcggcctga gcttgttttt cgtagctgcg
    12901 attgcgccat gcacgccagc ggcgcacagt gcccacagtg acgccttcgg gcatgaagat
    12961 catgatcacg accaggatca gaccatagaa tataatttcg tgctctccgc tggctccctc
    13021 catcaaaagc cgcaattggc cgcgtaagat ttcttttgtg atcagaatga ctgccacacc
    13081 gaacggtgcg ccccagatgc tggagaggcc gccgactgcg gacatgacga ccaattccag
    13141 cgagccgatg aaactgaagg gtgccggaga aactgaggct tgaaaatgtg catacaggct
    13201 gcccgccagg ctggccagaa ttgcgctcaa ggcgaaaatg cgcgctttgt aggcgctcac
    13261 atctacaccc attgcttgag cggcgacctc actcccgtga attgcccgca gtgcccgccc
    13321 ggtgcgcgag ttgatcaggt tgagaccaaa ggcaatcacg agcagggcta ccaaccagac
    13381 aaagaagtag taacgtgata tcggccagat ttcaaagctc cccagccgca ggcggggaat
    13441 acctggaata ccgtcgaatg tacccgtgat gtgttggcct tggaacccca tgttctcccg
    13501 gaataagata tagatcatca ctcccaagcc cagggtagcc attgccagat agtgaccccg
    13561 caggcggaga ataggacgcc caatcaagta ggccaggatg ccggcgaaaa tcatcccgag
    13621 caccatcacc aaccagggcc accaccagct gttgatcaag ctctcgggcg ctccaaataa
    13681 acttcccgcc cgtgcggtga agatggcgct ggcgtatgca cccattccat agaacgcagc
    13741 gtgcccaagc gagacctggc ccgcgtatcc catcaacagg ttcaagccta ccaatacgat
    13801 ggtcagaatc gccactctga tcatcgtatc tagggtgacc ataccccggg tgatatcgga
    13861 gaagtctagc ccgaaaggag aagcgagctc cacccaaccc aagacaccta ccacgacggt
    13921 cagcagcagc catccgagta gattttggtt tggcgcagat ttagcggtag gttccattaa
    13981 tgatcttctc catctgcttc aaccgccgtc ttgcgtacac caagcaatcc ttgcggacgg
    14041 aagagtaagg ctaaaattaa taggataaat gcgtagatgt ccttcatgcc ggctttggtt
    14101 accccagcag caagattttc tattaaacca agcatgaggc cacccaaaac tgcccccggc
    14161 gtgctcacca agcctcccag tattgcagcg acgaagccct tgagccccag ggagagaccc
    14221 atgtcgtaag aggggcgcaa aatgggagca accacgatgc ctccgatggc tcccagtgcg
    14281 gctgccagcg caaaggctac tacggacatc ctgctggtgc tgatacccac caggcgtgcg
    14341 ccccgccggt tgacggcaca ggcgcgcatg gcttttccga tcaaggtgcg ctcgaaaaag
    14401 aacgctaaag cgatcagcac cagtgccgta atcccccaaa tccaaaaact ttgtgacttg
    14461 atcacgatgc ccatccacct gatggtgcga tcagccatct ctagagtcgt aaaggcaggg
    14521 tagatataag catctgcccc ccaggtcagt aaagccaagc cttgggtggc gatgtaaata
    14581 cccaccgtga tgatgatgag ggtgagggta gatgaagagc gtgctgggtg gatagacagc
    14641 ctttcgacca gtattccaat caagctagtc aacaaaattg ccagcaaaac gctaataagc
    14701 aaatgcatcc aggccgggaa cgggagttcc cattcgtaga tggtcactgc cagcatcgcc
    14761 cccagcatga cgaagcctcc ttgggcaaag ttgatgatgc ctgttacgtt gaagaccatg
    14821 acgaaaccca tggcgatcag tgcatagatc gcgccgcctc gcaatccatc aatggttaat
    14881 tgcgcgtact gttgtaagcc gaaattctta cgccctccca aaaacaccgt aagaagtatc
    14941 aacgccagga agatgccaag aaagataatt gtctggacta gcttgcggcg cgattgttgt
    15001 tccatctagt tttttccttc ctggaggccg tagattctac ggcctccagg tatgaattga
    15061 ggtgttacta ccactcttcc tcagggaagt atatccattt gccgccttcg acctttacga
    15121 aggttagact ttcgaagcca agactgaagt ggtcctcagg cgagaggttg tacacgccgc
    15181 tgattccagg ccagtcttta acaccggtct cgagaaaatc acgcacggca gcacgttggg
    15241 cggctagatc gagcccatcg ggcagtgagg acactgctgt tacagccagc atgaccgcgt
    15301 cccagccatg accgccgaag gtgttgacgg gtttgcccgt ataggcggtg tagtctgtaa
    15361 cgtattgcac caggacatct ttctgcgggt cgtcatcggg tagcgtctcg ctaaccatga
    15421 ctctaccgca gggactgacc atgccctctg ccacttctgc acccatcaaa tcgatatgat
    15481 cttggttgca ggttccgtga ccgtggatga tgggcatatc tggtagcgca tcccgcaggg
    15541 cgatggagat gttcgatgaa gcgggcggga tcgcccccac caccactgcc tggcagtcgc
    15601 tggtttgaac gttgatcacc tgtgggaact cggtccccgt gcgctcgaaa gagtctgagt
    15661 acacgatttc gatccccgcc ggctcaaaag ctttttgagc ctgcgccagg gtgtctccgc
    15721 cataaccgct gttttcagac aggtggcaga ctttcgtgta tcccagttta ttgaggtaca
    15781 aggcctgcca ttgtgctgaa gccaggttgt cttgggctgt cttgaaagcc catttacgca
    15841 cctcaccggt ctctgggtcc ttcacaatag cacctgacga tgccatcgat agatacggaa
    15901 cctcggcttc agtaataatt ggcacgatgg ccatgctgat tccgctggtg gtgcccgcca
    15961 cgacgacttg cacgttctct tcctcgatca tgcggcgaac tacactgaca gccacatctg
    16021 ggttggtctc ggtgtcgaag atgacgatct ccacgggatg cctcacccca tccgggccga
    16081 ccacaccacc ttgagcgttg agttgctcgg cgatcatttg acctgtttca tgttcaggag
    16141 cccccaagct tgacccaggg cctgtgattg caggtgcaaa gcctatcttg taggtctcac
    16201 cgacaacttc ttcggtaggc tctggcgtcg gctcttcggt catggcaggc gcctctgtag
    16261 gcggaggggg ctcagtgggt tccggagcag acgtcggtgg gggtggcggt ggctcttcag
    16321 tgggttcagg tgttgcctga gcgcacgatg ccaggagaag cgccacgatc gccaacaagg
    16381 caaatagagt atttagtttt cccttcatcg gtttcctcca atttctcgat cagaattgtg
    16441 ccaatagatg catctttctt gagctcacac agttcacgac gagtctttca cctcctttct
    16501 atttcacaca agcgttcatg gttgcggtgg acctacctta atagtcttgt tcagggtttt
    16561 aagatttctc aaagcgatat gcttatggca ttccttttga aaaatcccta cacatgatca
    16621 tgaccgcgtc agcagtgtgt cctctatttc ggattctttg atctccgctc cgctcttcaa
    16681 gcgttctcgc atcaccgcaa ccatgtggag tacatcctcc tcggatagca gacgtttaga
    16741 acgcttcgca gccgctagaa ttttctcgac atatacgggg tgctcttcga tgccacgctg
    16801 gtgcaggtag aagcgcacgt tgtgctcccc agacatggga ccgatttcga tgccctgtac
    16861 acgccccacc atggaagcgg gaactccgca gtagactcgg tccgccagcc aattttcgtc
    16921 ccggctgagg gccttcacca ccgcggctgc gtggacaccg gttgaagtgc ggaaggaatc
    16981 agcccccacc accggatagt taaagggtac tggggcgccg cacttctccg cgatgatgtt
    17041 gcagtattct ggcaggcgtc ttaggtcgcg agttgctaac cccatgagat ttaagttaac
    17101 caggagcagc tccatcggag tattcccgct gcgttcgcca acacctaaac cgcaggcatg
    17161 cacccggtct gcgccagctt cgatcgccac cagggtattg ataatgtcca tgccgcgatc
    17221 ccggtgtcca tgccagtcga tacctacctc aggattgatc tcatcaacta ttttacgcag
    17281 gtacgagacc agggctcgca cgccttcggg cgtggcgtgc ccgacagtat ctgagacgca
    17341 caggcgctga gctccggctt ctatggccgc ggtatagacc ctccgcaagg tatcgggatg
    17401 ggctcgggtg gtgtcctcgg tgacaaacat gaccggtaag ccatgattgg tggcaaagga
    17461 taccgattct ttcgttcttt ccagcaagaa ctccacatcc cagccctctg cgtattgccg
    17521 gattgggctg gttcccagaa agatacacgc ctcaatgggg atgccggctt cctgagaggc
    17581 atcaacgatg ggcgtgatgt catccagcat tgtgcgcgcc gcactctgcg gctggatggg
    17641 catattctgg ttggcaattt ctttcgccaa aacgatcacg tcgtacttga agcgctcgcc
    17701 cgcgccgcac agcccaaggt cggctgcgtc gatgcccaga tctcgcatca ggtacaggag
    17761 atacacctta tcctgtattt cgggatgggt gatcgaaggc gattgcaatc catcgcgcag
    17821 cgtttcatct gtcacaagga tgcgcctctt tgggggctga aattcctcaa tttcgttcca
    17881 gtcatagatc aattggctta catcatcatt tgttgtaccg gacattgcta cctccttttt
    17941 ctgtgagtga gggaaattga ctgccctcat ggtttaccta tactttttgt tctgtgttcg
    18001 tagatacaaa tccttctgca atgtctattt ggttacgacc aatttcaaag gcttgtagat
    18061 tggcgctcaa gtagcgtttg ggaatgcgca aatgcagtgt cttatcccag atatccagtg
    18121 ggattggcaa cagcattgat aagccgccca ggatgactac atttgccatg cgaccatcgc
    18181 ccagatcgcg ggcgatagca gatgctggca tagtgatcac ccgcagcgat ttattcttca
    18241 agtaatcgac tgtttcatgg gggtaaagct gctcagcccc ttgcaccgag gcaggaacga
    18301 tctcaaaatc gtttgccagg atcacgccat actctgggtt agcgaaatgg ccgaagcgca
    18361 gggcttctaa cttttccaga ccgacaacca catccgcttg gcctgcggtg accaggggag
    18421 aatacacctt ctcagcaaag cgaacatgag tttcaacgct gccgccacgc tgtgagacgc
    18481 cgtggacttc ggtttgcttc gcatcataac cagcctgtac agcagccagc gaggtggcat
    18541 ctgcaatcag taaagagccc tgtccaccaa ccccagcgaa tatcaaacta tatactttac
    18601 tcatctcgtt tgcctccctc atgcggttac ttgtacgtct gccagatgaa tggcgtcggt
    18661 agggcatacc tgagcgcaga ctgtgcaggc agtgcactcg ttcgccacga tgtaggggaa
    18721 gttgttctcg ttgcgtttga tcgccgggca ccacacctta aagcaggcct cgcactgggt
    18781 gcacagctct tcttccacca cataagggat gacttcgcgg cgtttcattt cgggtagctg
    18841 ctggcaaggt ccttgggcga tgaccaccga agggccatca tttgccaggg cttgctgaat
    18901 cgaccgcagc acctctttcc gttgccaggt atccaccact ttgacgtgct ccacacctgc
    18961 agcgcgcacc acctctacca ggtttagttt tggggcgggt ctgccctggg catcttgccc
    19021 agaagcggga tgtggctggc tgccggtcat accggtggct gagttatcca ggatgatcag
    19081 gttcaggttg ctgccgttgt acaccgcatc aattaatggt gggatcccag cgtgtacaaa
    19141 ggtggaatct ccaatgactg ccacgacccg cttataaccc gctcgctcga gacccaccgc
    19201 ggtgccgatg ctggctccca tgcaaaatgt ggtgtgttca gcttcgtaag ggggtaaagc
    19261 gcccatagtg tagcaaccga tatccccagc aacggttact tttagctggc gcaaagaagc
    19321 aaagaccgtg cgatgaccgc aaccgatgca cagcataggt ggccttggca gcagtgtgat
    19381 gccttcagtg aatggctctg gggtctttcc catgcccagg gcttccatca gccgcccggg
    19441 agaatactcg ccgatgactc caaagagctc cttgcccacc aggtttgtta tccccaggct
    19501 gcgcataaac tcttcgatga acggctcgcc ttcctcaacg atatacactt tctcgtagcg
    19561 ggagcagaag tcacgcagca aattttctgg tagcgggaaa gtcatcccca ggcggagcac
    19621 gccagcttct ggcatcacct cttttacata ctggtaggct atccccgaag tgacgacccc
    19681 caccgggttt tctgtgtgtt cgatacggtg cagagggctg gtttcggcgt actctttgag
    19741 agcttgcaag cgctcttcaa ctttgggcct gtgcaagcgc cggtaaatag gaacggaaaa
    19801 ccgcttcgca tctgggacaa aatcccgctt cggcacatct ttgcgctcac cgatctccac
    19861 cagacccttg tggtgcgcca ggcgcgtggt ggtgcgcagc atgactggaa tgccatactt
    19921 ctcggacagc tcgagaccgg cgataacaaa gtccttgcat tcctgggcat cgcttggctc
    19981 caaaacgggg actttcccca ttcttgccag gtaacgatta tcttgctcgt tttgggagga
    20041 gtgcatccct gggtcatcgg cagacacgac cacaaaaccg ccgttagtgc ctgcgtaggg
    20101 gaataccatg aaggcatccg ctgcgacgtt caggccaacg tgcttcatgg tcaccaggac
    20161 gcgcaatccg ccgatagcgg ctcccatacc ttcctcaaat gctaccttct cattcgccga
    20221 ccagcgcggg ttggtctcag taaagcgcgt tgccagcgtc tcaataatct cggtgctggg
    20281 agtgcctgga tatcccgcgg cgaaggttac cccggcttcc catgcgcctc gggcgatcgc
    20341 ctgattgcct aacaataatt ctcgcataga ctctccttca aaatgcaata ctccagctcg
    20401 ctaccgatta ggagggtcgc tcactggtgg agtgaccttc acgctctgaa acgcctaaga
    20461 ttttggcggc gttgttgaat aaaatatcct gcttggcttg ctccgttata ggtaaggaca
    20521 ttatggcttc tacgttgcgg cggaaatcgg ggttgccggg ccaatctgag ccatagacta
    20581 ctttgtcggt gatctcctcc aggcggggga agtattccag gagtttcttg cctggcagac
    20641 cggagacttc catgtacacg ttcgaatgct ggcgcgccat ccaaaacgcc tgctcgtacc
    20701 agaagggtcg tccagaatga gccattaaga tctttagatt ggggaaatcg ttggcgacgt
    20761 cgtccagcag cagtgggtcg ccatacttga tgcgcgtccc cttgaatacc gaagagccgg
    20821 tgtgcaccaa gacaggcagc cccagttctt gggcgcgggc gtaaagaggc gatagccgtg
    20881 cgtcgttggg atagtggtgg tggtaggttg ggtacacttt gacgccccca aagccatact
    20941 cgtccaccag gcgtgtcagc tcggcgccta gatcgttgac aatgaatggg ttgatgctgg
    21001 caaacgggat cagccggccg cgcggccctg aggggggatc ggggagcgcg ttggtctgca
    21061 tgcatagctc gcccacgtac tcgttgctcg cccacccggt agtgactggg gatacctccg
    21121 ccaatcccac cgcccaatcg atgtggttgg cttgcagggt gcgccgcagc ccagaaggag
    21181 tcagcacttc ttcgacaaaa gcatcgatgt ctcccgaaaa gtttgatcgg atccactcca
    21241 taacccacgg taattcatgc tcaggacggc tgagatgaat gtgaaagtca ataatcatgg
    21301 cacaactacg ctgcgaatac cctcgcctct tctcaaaccg tcaaaggcct cattgatctc
    21361 ttccagtgga tagcgagctg tgaccagctc tttgaccttg atctttccct ggcgaaccag
    21421 gtcgataacg cgtggatagt caaccgctcg acagcccaaa gagccgatga tctccatttc
    21481 acggtacatt accctgccag tatctagcgt catcggtttg gcggagaatc ccacaaccac
    21541 gaagcgccca ccagtgcgca gcgaggcaaa ggtttgcgcc tgaacaaccg ggttgccgat
    21601 ggcttcaaag cctttgtcag caccgcctcc cgtcaacttg cggatttctt tatcaatacg
    21661 ttcgaaattc tgggcgttga gcgccacctg tgcaccgagt tgtttggccc actctagctt
    21721 ggtgtcgata atgtccactg cgatcacttg tgcgcccagt gccgccgcta cctggataac
    21781 gttcaagccg atcccgccac agccgaagac gaccacatag tcgcccggtt ttacctgacc
    21841 gcggttgacg acggcatgat agggggtggt gatggcatcg gcgatgatgg ctccctccac
    21901 caggggtact tcctcgggga ggcggagcgc atctttagct ggggccagca tgtactctgc
    21961 gtagcctccg tcaacgttgt tgccgaacat caccatcctt tcacagatgt tctctcgccc
    22021 ttcacggcac atggcgcact ccccacagcc ataaacagct ggtagcagca cgcggtctcc
    22081 ttctttccac tgctcaaccc cggcgcccag ccctgcaatc gtgcctgaga cttcgtgtcc
    22141 cagtactagc gggggtttct taaaagtagg gactccatgg tcaaggtaat gcaagtcggt
    22201 gtggcataag ccgcacgccg cgattttcac cagcacct
//
LOCUS       DBNGSX010000471         5907 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_739753, whole genome shotgun sequence.
ACCESSION   DBNGSX010000471 DBNGSX010000000
VERSION     DBNGSX010000471.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5907)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_739753"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..879)
                     /locus_tag="AC16CB_13945"
     CDS             complement(25..879)
                     /locus_tag="AC16CB_13945"
                     /inference="COORDINATES: protein motif:HMM:NF012402.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HIR3343619.1"
                     /translation="MTTTNTPHNENSMKPSLGRLAYIKDLGTEIKLFGVQLLNGNGAD
                     FHAYHPGQFAFVSAFGIGEAAFGFASIPRNGTLEFAIQRLGSVTTELHELGVGDTVGV
                     RGPLGNCFPLDSMRGKNLIVLGGGIGGAPLRPVIQYVLANRADYGHLTILWAARHPSL
                     LVFTDEYDTWRSAPDTQLHLTVDVADTEWDHHTGLITDLLERVSPSPENTITITCGPP
                     IMIHFVNKMLIEKFGFRPEQNYVTLEARMHCGIGKCGRCNLGEKLVCVDGPVFTMAEV
                     GNFLESFL"
     gene            complement(876..1883)
                     /locus_tag="AC16CB_13950"
     CDS             complement(876..1883)
                     /locus_tag="AC16CB_13950"
                     /inference="COORDINATES: protein motif:HMM:NF028488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3343620.1"
                     /translation="MKYISRSDLSIILNELIGESRLIAPKKVGGVILYQPITDTSHIT
                     WDFTRPVLSIKESFFPATERLFTIDINGQEVTLNDTLPEIPQIILGVRPCDARGMKIL
                     DALFIDTPPRDSYFAQHRANTTIIGLSCQELGETCFCTSMDGSPTDPQGMDVMLTEIE
                     DGYSVQIITEKGKKFFGDHLSLAYEEATPPSGTLDRPSAIPAPNDINWPRKFHEGYWD
                     QLAERCLSCRICAYICPTCRCFTVRDEPIASNDGMQQFERIRCWDACTSEAYRQIAGG
                     HNPRQAKKQRLRNRIYCKFDFYPKQYGPIACTGCGRCVEMCPVNIDITEVLRDLAEVT
                     P"
     gene            complement(1880..3268)
                     /locus_tag="AC16CB_13955"
     CDS             complement(1880..3268)
                     /locus_tag="AC16CB_13955"
                     /inference="COORDINATES: protein motif:HMM:NF014692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HIR3343621.1"
                     /translation="MMTFEPKIITFLCTWCSYTGADTAGIARLKSPSNIRAIRVPCSG
                     RVAPEIIMKTFDQGADGVLVLGCHIGECHYDSGNHRTAKRLPILRSLMAFAGLEPERL
                     HLDWVSASEGERFSRIVSEFTESVRLLGPARWKSENRQPRFDQTQLAIDRSYTFDQSS
                     YSEEVERSISFTNNLQAKARELLSCGKVACVIGYEKGTHNRTRPVFIYTEDAVERLVW
                     NKDCTHNLTVYLRRAIYPPLGKQNGEPGKSVAIVAKPCDSRAINVHLAGNHFQRDQVH
                     VIGMACEGILDKQQQYRMDHILQERCLHCTERLPVIYDTLIGEPPANQVEPTTSARLY
                     YLETLSPIERMDFWLSHFDRCIRCYACRQVCPMCDCPTCLFERDDSLWVGPGIRLDEK
                     RIYHLGRAYHLAGKCVDCHECERVCPVNIPISLINSKLNLEMEKFFGYRAGEQAIPSP
                     LLTILSGEERLP"
     gene            complement(3265..5766)
                     /locus_tag="AC16CB_13960"
     CDS             complement(3265..5766)
                     /locus_tag="AC16CB_13960"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3343622.1"
                     /translation="MALLRQKRYADSYWAIRREHPFPSVCGRVCNHRCEDACSRAKYD
                     EPVNIMGLKRFIADWAFHHRDQLPSMRDKSLAGTPFQHHHPPTGKKIAIIGAGPAGLT
                     AALDLVRLGHAITIFDALPVAGGMMRVGIPPHRLPVEMLDWEIQQIIDEGIDLQLNTW
                     VDDIPALLDNGYNAVLIATGAQQAKKLPIRNSNHPGNWLSLKLLRSVALGEKINLIGK
                     KVVVLGGGNVALDTARTVLRLGASQVRMACLEPRGEMPGFEWEVKVAEDEGVIFCPAR
                     TFKEIVVENEKISGVRCAEVIFHGFKRGRPDIEEISSTEHILPADLVIWAIGQGPDFS
                     FLPPDGSINTRYPVGIQSNMDMMTTQPGVFVAGDVHRGVTFFVVDAIGEGHKAARSID
                     RYLKGRNNITEPSKLPTVELDLEEIRSKLAHGEASSKARIPIRSIPLEERRHNFREVD
                     LTMSEEEALAEAERCLRCGICSECLECVVACERGAINHNMREETIELKVGTIILATGF
                     TDFNPSQLSELGYGNLDNVLTAMEFERLINASGPTGGKVLLKNGQQAKSVAILHCIGS
                     RDVANNEYCSRACCMYSLKIAQLVNDYMEAEVHEVYRDMRTFGKDYEEFYNRTRQKGI
                     NFYHGKVREISRRNGKLRVAWDENFYNQPDHVDVDMVVLATGFEPQSDTAKVASIFGV
                     SRSRDGFFLERHPKLAPIETANEGVFLAGACQSPKDIPDSVAQAGAAAAAALSLIDQG
                     TIALDPSIAEVNHLHCAGCGQCSNACPYGAIEVKDGAAVINGFLCKGCGTCAGSCPNK
                     ALTLIHFDDRQIISEMIGALKAPVATSVLSVQEAG"
     gene            5703..>5907
                     /locus_tag="AC16CB_13965"
     CDS             5703..>5907
                     /locus_tag="AC16CB_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343623.1"
                     /translation="MGVRAELPSRNLRIASDVGGRYILERAGLSGRRFGRVQYLISVL
                     CMLLGPIVLKNDRTLFACLNPNRM"
BASE COUNT         1446 a         1483 c         1430 g         1548 t
ORIGIN      
        1 tagaggacag gttgttcctt atccttacaa gaagctctcc agaaaattac ctacttcagc
       61 cattgtaaat accggtccat ccacacaaac tagtttctcg cctagattgc atcgcccaca
      121 cttgccaatg ccgcagtgca tgcgagcctc cagggtaacg tagttttgtt ccgggcggaa
      181 gccaaacttt tcgattagca ttttgttgac gaaatgaatc atgatcggtg gtccgcatgt
      241 gatcgtaatt gtgttttcag gagaaggtga aactcgttct aaaaggtctg tgattaagcc
      301 tgtatggtgg tcccattctg tatcagcaac atccacagtc aggtgcagct gagtatctgg
      361 cgcactccgc caggtatcgt attcgtcagt aaataccagc agggaggggt gacgggctgc
      421 ccagaggatg gtaagatgac cataatcagc gcggtttgcg agcacatatt gaattacggg
      481 gcgtagaggt gcccctccaa taccaccacc taaaacaata agatttttac ctcgcatact
      541 atcaagcgga aagcagttgc caagcggacc ccgcacccca acggtatcac ctacaccgag
      601 ttcgtgcaat tcggtggtca ccgagcccag gcgctggatg gcaaactcaa gggttccatt
      661 cctgggaatt gaagcaaatc caaaggcagc ctcccctata ccaaatgccg atacaaaagc
      721 aaattgccct ggatggtaag catggaaatc agcaccattg ccattcagta attgaactcc
      781 aaacaacttg atctcagtgc ctaaatcttt gatataagca agccgtccca aactgggttt
      841 catagaattt tcgttatggg gagtatttgt tgtggtcatg gtgtcacctc tgctaaatcc
      901 cgaaggactt cggtgatatc gatgtttacc gggcacattt ccacacaacg cccacatccg
      961 gtacaggcta tcggaccgta ttgctttggg tagaagtcaa atttacaata aatgcggttg
     1021 cgcaatctct gtttctttgc ctggcgagga ttgtgacctc cggctatttg tcgataggct
     1081 tcgctagtgc aggcgtccca acagcgaatt cgttcgaact gctgcatgcc atcattgctg
     1141 gcgatgggct cgtctcgcac ggtaaaacaa cggcatgttg ggcaaatgta tgcgcagata
     1201 cgacaactca ggcagcgctc tgctaactga tcccagtaac cttcatgaaa cttacgaggc
     1261 caattgatat cgttgggtgc agggatggca gatgggcgat caagagtgcc tgagggggga
     1321 gtcgcctctt cataggccag tgataaatgg tcaccaaaaa acttcttgcc tttttcggtg
     1381 atgatctgga cagaatagcc atcttctatc tcggtgagca tcacatccat gccctgcgga
     1441 tcggttggag agccatccat gctggtacag aagcatgttt cgcccaactc ttggcaggac
     1501 aatccaataa tggtggtatt ggcacgatgc tgtgcaaagt atgaatctcg aggtggggta
     1561 tcgatgaata aggcatcgag gatcttcata ccccgggcat cgcatggcct gacgcccaaa
     1621 ataatctgag ggatttcggg caacgtatcg tttaatgtga cctcctgccc gttgatatcg
     1681 atggtaaata ggcgctcggt ggccgggaag aagctctcct tgatcgatag tactgggcgg
     1741 gtgaagtccc atgtgatatg gctggtatct gttatcggct gatataagat caccccgccc
     1801 acttttttgg gggcgattaa tcttgactcg ccaattagct cattcaatat tatggagaga
     1861 tcgctacggg agatgtattt catggcaatc gctcctctcc gctaaggatt gtaaggaggg
     1921 gagatggtat agcttgttct ccggcgcggt acccaaagaa tttctccatc tctagattta
     1981 gcttgctatt gatcaaactt ataggtatat tcacagggca cacgcgttcg cactcatgac
     2041 aatcaacgca tttgcccgca agatggtaag cccgacccag gtggtaaatt cgtttttcat
     2101 ctagcctgat gcccgggccc acccaaagcg agtcgtcccg ttcaaaaagg caggttgggc
     2161 aatcgcacat cggacagact tgtctgcagg catagcagcg gatgcagcga tcgaaatggg
     2221 aaagccaaaa atccatacgc tctatcggtg ataaagtttc caaataatat aagcgcgcgg
     2281 aggttgtagg ctccacctgg ttcgctggtg gttcaccgat gagcgtatca tagatgaccg
     2341 gcagacgctc agtacaatgc aagcagcgtt cctgaaggat gtggtccatt cgatactgtt
     2401 gctgtttatc aagaatccct tcgcaagcca ttccgatcac atgcacctgg tcgcgctgga
     2461 agtgatttcc cgctagatgt acattgatcg cccgactgtc gcatggttta gctacgatgg
     2521 caaccgattt tcccggctca ccattttgtt taccaagcgg tgggtaaatc gccctgcgca
     2581 ggtagactgt caggttgtgt gtgcagtcct tattccagac cagacgctcg accgcgtctt
     2641 cggtgtatat aaagactgga cgggttcggt tgtgagtgcc tttttcataa ccaataacgc
     2701 aggcaacctt cccgcaagag agcagctctc ttgccttggc ttgtaggtta ttagtaaagg
     2761 atattgatct ttctacttct tctgaatatg agctttggtc aaaggtgtat gatcgatcaa
     2821 ttgccaattg agtttgatca aatctaggtt gtctattctc cgatttccat ctggctggcc
     2881 cgagcaatcg aacgctttcg gtgaattcac tgacgatacg gctaaatcgc tcaccttcgg
     2941 aagcggatac ccaatccagg tgcaaacgtt caggttctag cccagcaaaa gccatgagtg
     3001 atcgtagtat aggtagcctc ttagccgttc ggtgattacc gctatcataa tggcactcac
     3061 cgatatggca tcccaacacc agaaccccat ctgcaccctg atcgaatgtt ttcataatga
     3121 tctctggagc cacccgacca gaacatggaa cgcgtatagc ccgtatattg gacggagatt
     3181 tcaagcgtgc gatgcctgct gtatccgctc ctgtgtagct gcaccatgta catagaaatg
     3241 tgatgatttt aggctcaaat gtcatcatcc cgcctcctga acgctcaata cagaggttgc
     3301 aacaggtgct tttagtgctc caatcatctc gctaatgatc tggcgatcat caaaatgaat
     3361 gagagtcaaa gctttattcg ggcaactccc agcacaggtg ccacaacctt tacataggaa
     3421 gccattaatc acagcagccc catcttttac ttcgatcgct ccataagggc aggcattcga
     3481 acactgtcca cagccagcgc agtgaaggtg gttaacttct gcaattgaag ggtctaaggc
     3541 aatggtgcct tgatctatta gagagagcgc tgcagccgca gctgcgcctg cctgagcaac
     3601 actatcgggg atgtcctttg gactttgaca agcgcctgct aggaatactc cttcgtttgc
     3661 tgtttcgatt ggggcgagct tagggtggcg ttcgaggaaa aacccatctc ggctacggct
     3721 aaccccaaaa atgcttgcaa ccttggccgt gtcactttgg ggttcaaagc ccgtagccag
     3781 tacgaccatg tctacatcaa cgtggtctgg ttgattataa aaattttcat cccaggcaac
     3841 cctgagtttg ccattgcgac gagaaatttc tcttacttta ccgtggtaaa agtttatgcc
     3901 tttctgtcgc gtgcggttgt agaattcctc gtaatctttg ccgaaagtgc gcatgtcccg
     3961 ataaacttca tggacttcag cctccatgta gtcatttacc aattgagcaa tttttaacga
     4021 gtacatacaa caagcccgcg agcagtattc gttgtttgcg acgtcgcgac tacctatgca
     4081 atgcaagatt gctacactct tagcttgctg accgttttta agaagcacct tcccgcctgt
     4141 tggaccgctt gcgttgatta accgttcgaa ctccatcgcg gtgagaacat tatccaaatt
     4201 gccatagccc agttcagaaa gttgtgatgg attgaaatcc gtaaatccgg ttgccaggat
     4261 aatcgtgcca acttttaact cgatggtttc ctctcgcata ttgtgattaa ttgccccacg
     4321 ttcgcaagct accacgcact ccaggcattc tgagcaaatt ccacatcgca gacaacgctc
     4381 tgcctcagcc agcgcctcct cttcactcat ggtgagatcc acctcgcgga agttgtgtct
     4441 cctctcttcg agagggatac ttcgaatggg tatacgtgct tttgaagaag cctccccatg
     4501 ggcgagtttt gagcgaatct cttccaaatc aagctcgaca gttggtagtt tggacggttc
     4561 tgtaatgtta ttcctgccct taagatagcg atctatgctg cgggcagcct tatgtccttc
     4621 tccaatggca tctaccacaa agaacgtcac accccgatgt acatcgccag ctacgaagac
     4681 acctggttga gtggtcatca tgtccatatt gctttggatg ccaactgggt atctagtgtt
     4741 gatactgcca tcagggggca agaaggaaaa atctggccct tggccaattg cccagatcac
     4801 cagatcggca ggcaagatat gttcggtgct agaaatctcc tcgatgtccg gtcgcccacg
     4861 tttaaagccg tggaatatca cttctgcaca gcgtacacca gatatttttt cattttctac
     4921 aacgatctct ttgaatgttc tggcggggca aaaaatcaca ccttcatctt cagcaacttt
     4981 tacttcccat tcgaagccag gcatctcacc gcgcggttcc aggcatgcca tgcgaacctg
     5041 gctggctccc aagcgcagca cagtgcgtgc ggtatccagg gcgacatttc ccccacctaa
     5101 gacaaccact ttcttgccga ttagattaat cttctcgcca agggcaacgc ttcgaagcaa
     5161 tttcaggctg agccagttgc cgggatggtt ggagtttcgt ataggtagct ttttggcttg
     5221 ctgtgcacca gtagcaatca aaacagcatt gtagccgtta tccagcaaag caggtatgtc
     5281 atcgacccac gtattaagtt gcaaatcaat gccttcatct atgatttgct ggatttccca
     5341 atcgagcatc tctactggta acctgtgggg agggatgccc acccgcatca tgccacctgc
     5401 tactggtagg gcatcaaaga tcgtgatggc atggcctaaa cgaactagat ccagcgcagc
     5461 ggttaatcct gctggaccgg caccaattat tgctattttc tttccagtag gcggatgatg
     5521 atgttgaaat ggtgtgcctg caagactttt gtcgcgcata cttggaagtt ggtctcgatg
     5581 gtgaaaagcc caatcggcaa taaatcgttt taaacccata atattgactg gctcatcgta
     5641 cttggcacgg ctgcacgcat cttcgcaacg atgattgcac actcgtccgc aaacggatgg
     5701 gaatgggtgt tcgcgccgaa ttgcccagta ggaatctgcg tatcgcttct gacgtaggag
     5761 ggcgatatat ccttgagcgc gctggtctgt cgggcaggcg cttcggcagg gtgcaatacc
     5821 tgatttctgt attgtgtatg ctcctgggac cgattgtgct gaaaaacgat aggacgcttt
     5881 tcgcgtgctt aaacccaaat cgaatgg
//
LOCUS       DBNGSX010000472         3268 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_393147, whole genome shotgun sequence.
ACCESSION   DBNGSX010000472 DBNGSX010000000
VERSION     DBNGSX010000472.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_393147"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="AC16CB_13970"
     CDS             complement(<1..226)
                     /locus_tag="AC16CB_13970"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3343624.1"
                     /translation="MRGLPKLFDLTGRVAVITGGAGLLGAEFCLTLAEAGAAVVIADL
                     NEGGALALAESLSQGGKRALAVKTDVTFPDS"
     gene            complement(229..2001)
                     /locus_tag="AC16CB_13975"
     CDS             complement(229..2001)
                     /locus_tag="AC16CB_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sulfate
                     adenylyltransferase/adenylylsulfate kinase"
                     /protein_id="HIR3343625.1"
                     /translation="MSKQSNNLISPYGGKLVNLVVSGEEREELLAKSSRLPSVQISSR
                     SLCDLELLATGAFSPIDRFMRKNDYERVLTEMRLHDGTLFPIPVTLPVDESALPSWGE
                     QITLSDSRNNTLAVMQIEEVYHWDAQREARLVLGTTDPRHPLFSEMVRWGKVYVSGEL
                     KIIDLPKYHDFQELRRTPAQVRAIMGKMGNGNVVAFQTRNPMHRIHEELTKRAAEGVN
                     GSLLIHPVVGMTKPGDVDHYTRVRVYRALAENYYEKNRTLLSLLPLAMRMAGPREALW
                     HAIIRRNYGANYFIVGRDHAGPGNDSHGRPFYGPYEAQMMVSQYANEIGVQPLEFKEL
                     VYLADDERYEEQDKIPEGARIFNISGTQVRDDYLAKGKSLPEWFTRKETAEILQQMYP
                     PRHKQGVCIWFTGLSGSGKSTTAEVLTALLLERGRQVTLLDGDVVRTHLSKGLGFSRD
                     DRDTNILRIGFVAGEISRHGGTVICAAISPYRATRNEARKMVGEEHFIEVFVDTPIDV
                     CEQRDVKGLYARARRGQITGFTGVDDPYEIPVDPEITLYTVEVSPEENAQKIVDYLEE
                     RGYLLPDGSLNAKQEEQQTEPTPA"
     gene            complement(2092..2964)
                     /locus_tag="AC16CB_13980"
     CDS             complement(2092..2964)
                     /locus_tag="AC16CB_13980"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0070085 - obsolete glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HIR3343626.1"
                     /translation="MAKAVKIGDRWVGEGHPVFIIAEIGINHNGNLHIAKRLVDVAFM
                     AGCDGVKFQKRTPEKAVPPEFQNVMRETPWGIISYLEYRRMVEFGFKEYDDIDQYCKE
                     KGIIWFASCWDEDSVDFIEQFSPPCYKIASASLTDIPLLEKVKSTGRPVILSTGMSTL
                     EEIAASVELLGTEHLLIAHSTSTYPCPPEELNLRMIETLRERFDCPIGYSGHEVGLQT
                     TYAAVTLGAAFVERHITLDRAMWGSDQAASVEPGGMIRLVRDIRVIERALGDGQKRVY
                     DSELKAREKLRRRN"
     gene            complement(2974..>3268)
                     /locus_tag="AC16CB_13985"
     CDS             complement(2974..>3268)
                     /locus_tag="AC16CB_13985"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014674559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HIR3343627.1"
                     /translation="NPVVQARADKLGIPCLHGLQDKGLALGQLLHEQGIDPAQVVYVG
                     NDVNDLPCMAQVGCAVAVADAHPAVLAQANLVLEHSGGNGAIREICELILDSK"
BASE COUNT          643 a          939 c          947 g          739 t
ORIGIN      
        1 ccgaatcagg gaacgtcacg tcggtcttca ccgccaaggc gcgtttgcct ccctgggaga
       61 gagattcagc cagcgccagc gcaccgccct cgttcaagtc ggcgatgacc acagcggccc
      121 cggcttctgc cagtgtaagg cagaattccg cgcccaacag accggcgccg ccagtgatga
      181 cggcgacacg gcctgtcagg tcaaataatt tcggtagacc tcgcatgttt atgctggggt
      241 aggttcagtc tgttgctctt cctgtttggc attcaacgaa ccatcgggga gcagatagcc
      301 acgctcttcg aggtagtcga caattttttg ggcgttctcc tcggggctaa cctcaacggt
      361 atacagtgtg atctccggat caacaggaat ctcgtagggg tcgtccacgc cggtgaagcc
      421 ggtgatctgg ccgcggcggg cgcgtgcata caggcccttg acatcacgct gctcgcagac
      481 atcgatgggg gtatctacga agacctcgat gaagtgctcc tcgccaacca tcttgcgggc
      541 ttcgttgcgc gtggcgcggt aggggctgat agcggcgcag atcacggtgc cgccatggcg
      601 ggagatctcg ccggctacga aaccgatgcg caggatattg gtgtcgcggt cgtcacgcga
      661 gaagcccaat cccttggaga ggtgggtgcg cactacatcg ccgtctagca gagtaacctg
      721 gcggccgcgt tccagcagca gggctgtgag cacctccgcg gtggtggact tgcccgagcc
      781 actcagaccg gtgaaccaga tgcagacgcc ttgcttgtgg cgcggtggat acatctgctg
      841 caggatttca gcggtctctt tgcgggtgaa ccactcgggt agtgacttgc cttttgccag
      901 gtagtcgtcg cgcacctgag tgccagagat gttgaagatg cgggcgccct ccggaatttt
      961 atcctgctct tcgtagcgct catcatctgc cagatagacc agctccttga attcgagagg
     1021 ctgaacgccg atctcgttgg catattggct taccatcatc tgcgcctcat agggcccgta
     1081 gaaggggcgt ccgtggctgt cattgcctgg tccagcgtgg tcgcgcccaa cgatgaaata
     1141 gttagcgcca tagttgcggc ggataatggc gtgccaaagc gcctcgcgcg gcccagccat
     1201 gcgcatggcc aacggcagca ggctgagcaa tgtgcggttc ttttcgtagt aattttctgc
     1261 cagggcgcgg tagacgcgca cgcgggtgta gtgatccacg tcgccgggct tagtcatgcc
     1321 caccacggga tggattaaca ggctgccgtt cacaccttcg gcagcgcgct tggtcagctc
     1381 ctcgtgaatg cggtgcatgg ggttgcgtgt ttggaaggca accacgttgc cattgcccat
     1441 cttccccatg atagcgcgga cctgggctgg tgtgcggcgc aactcctgga aatcatggta
     1501 cttgggcagg tcgatgattt tcagctcgcc tgagacatac acctttcccc agcgcaccat
     1561 ctccgagaac agcgggtggc gcgggtcggt ggtgcccagc accaggcgcg cttcccgctg
     1621 ggcgtcccag tggtacactt cctcgatctg catcacggcc agcgtattgt tacggctgtc
     1681 gctcagcgtg atctgctcac cccagctggg cagagcgctc tcgtccacag ggagtgtgac
     1741 cgggatgggg aatagggtgc cgtcatggag gcgcatctcg gtcaggacac gctcgtaatc
     1801 attcttacgc ataaagcgat cgattggaga gaaagcccct gtcgccagca attccagatc
     1861 gcaaagcgag cgagatgaga tttgcacaga aggcaggcgg cttgatttgg ctagcagttc
     1921 ttcgcgctcc tcaccagata ccaccaaatt gaccagcttt cccccgtagg ggctgatgag
     1981 gttattactt tgttttgaca tacaatgttt ctccttgaat aattattttg cactatggca
     2041 tgatgatcac aagataactg ataaggcttg atgccattct tgcattttgg ttcaatttcg
     2101 tctccgcagt ttctcgcgag ccttgagctc gctgtcatat actcgttttt gcccatcgcc
     2161 cagggcgcgc tcgatgacac gaatatcgcg caccagtcga atcatccctc ctggctctac
     2221 agaggctgcc tggtcgctgc cccacatggc tcgatccagg gtaatatggc gctccacgaa
     2281 tgcggcaccc agggtcactg cggcgtaggt ggtctgcaga cccacttcat gaccagagta
     2341 tcctatcggg caatcaaaac gctccctcag ggtctcgatc atgcgcaggt tcagctcctc
     2401 tggcgggcat gggtaggtac tggtgctgtg agcgatcagc agatgctccg tgccgagcag
     2461 ctccacggaa gccgcgatct cttccagggt ggacatgccg gtggagagga tgaccggtcg
     2521 tccggtggat ttcaccttct ccaacagtgg tatatctgtt aacgaagcag aagcgatttt
     2581 gtaacagggt ggtgagaatt gttcaatgaa atccaccgaa tcctcgtccc agcacgaggc
     2641 aaaccagatg atgcctttct ctttgcaata ctggtcgata tcatcatatt ctttgaagcc
     2701 gaactcgacc atgcggcggt attccaggta agagatgatg ccccagggcg tctcgcgcat
     2761 cacattctgg aattcgggtg gtacagcctt ctcaggtgtg cgcttctgga acttcacccc
     2821 gtcgcagcca gccataaagg ccacatcaac caggcgcttg gcaatgtgta gattgccgtt
     2881 gtgattgatg ccaatctctg caatgatgaa aacgggatgt ccctcaccaa cccatcggtc
     2941 gccgattttt actgcttttg ccatgcaggc tccttactta ctatctagaa ttaactcaca
     3001 aatctctcgg atcgccccat tgccgccgct gtgctccagg accaggttgg cttgagccag
     3061 cacagccgga tgggcgtcgg ctactgccac ggcacatccg acctgcgcca tgcagggcag
     3121 atcgttgaca tcattgccca cataaaccac ctgggcgggg tcgatgcctt gttcgtggag
     3181 gagttgtccc agggccaggc ctttgtcctg cagcccatgc aggcaaggga tgcccagctt
     3241 atcggcgcgg gcctgcacca cagggttg
//
LOCUS       DBNGSX010000473         4813 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1362388, whole genome shotgun sequence.
ACCESSION   DBNGSX010000473 DBNGSX010000000
VERSION     DBNGSX010000473.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4813)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1362388"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="AC16CB_13990"
     CDS             <1..682
                     /locus_tag="AC16CB_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR3343628.1"
                     /translation="RLLRSWRNGRSQHNAYLEDYASLVLALISLYQSDPNPYWYTSAT
                     SLLDEMIQHFSDPSGGFFDTRIDHEPLITRPKDLQDNATPCGNSLAAMALLQHSNYSG
                     RSDLVDIAETSLRSIIDQSSKYPTAFAYWLCAADYVANPVSEVAIIGSAKDERTQALV
                     NVLWETYRPRTLSAQANLPISTEMPELLDNRELVDGLPAAYVCQNFVCNLPVTTPDEL
                     RIQLKRAA"
     gene            745..1242
                     /locus_tag="AC16CB_13995"
     CDS             745..1242
                     /locus_tag="AC16CB_13995"
                     /inference="COORDINATES: protein motif:HMM:NF013122.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR3343629.1"
                     /translation="MDTETTGYARDSEIVEVCVLDDQGEVLFESLVKPTQLIPRDAVR
                     IHGITNEMVSSAPSWMSVWPNLERVLSGHVVGIYNAEFDLRLIQQTHNKYRMKWASRA
                     DFTPFCIMKLYAQYYGEWNHSRGSYRWHSLEDAGRQCHIPLPNSHRARDDCLLARALL
                     HFMAS"
     gene            complement(1239..2027)
                     /locus_tag="AC16CB_14000"
     CDS             complement(1239..2027)
                     /locus_tag="AC16CB_14000"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR3343630.1"
                     /translation="MNLILERKMNIITWNVNGLRSVLRKGALDAILVRAPDILCFQEI
                     KSKPEQLNTDDHQLFEDYHAFWNPAEKLGYSGVATFARSESLDVSYGLGLDEFDREGR
                     VICSKYPEFLLFNIYFPNGQRDHGRLAFKLKFYAHLLDLCDSLHAANQAIILCGDFNT
                     AHKEIDLKNPKQNAKTSGFLPEERAWIDLYLEHGFVDAYRRLYPGRVQYTWWTYVGRA
                     RERNTGWRLDYFLVSENLMPRVQDVVIYDDIPGSDHCPVELFID"
     gene            2129..3418
                     /gene="hisS"
                     /locus_tag="AC16CB_14005"
     CDS             2129..3418
                     /gene="hisS"
                     /locus_tag="AC16CB_14005"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIR3343631.1"
                     /translation="MKSIIQPIKGTRDFYPELMTIRSWLYETAREISESFGYQEYDAP
                     FLEPIELYAAKSGEELVKEQSFVFPDRGGDLIALRPELTPSLARMVAQRQRQLIYPVR
                     WWSFGPFWRYERPQKGRSREFFQWNIDLFGINTPEADAELVAIACAYMQAVGLTPQEV
                     GILVNNRRLVDAELEALGIPPENRPIVSRWIDRRGKMSPDDWTSYGRDIGLSREQLDQ
                     MKALLANIDLWKKSEELVRFFDAIDALGCRQYVRYDPNIVRGLDYYTGIVFEAVDLQG
                     DVRRSILGGGRYDNLLADVGGDPMPGVGFAMGDMVIGLLLEKYGHLPESLDTSPAPVL
                     VTVFDDKSLLASFTLAAELRRAGINVNCYPEVAKLSKQFKFADRVGIRLAVVLGPEEQ
                     QSGMVTIKNLHSGEQITISRSSASSTISHWLEKPASP"
     gene            3438..3509
                     /locus_tag="AC16CB_14010"
     tRNA            3438..3509
                     /locus_tag="AC16CB_14010"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3470..3472,aa:His,seq:gtg)
     gene            complement(3619..4623)
                     /locus_tag="AC16CB_14015"
     CDS             complement(3619..4623)
                     /locus_tag="AC16CB_14015"
                     /inference="COORDINATES: protein motif:HMM:NF014646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3343632.1"
                     /translation="MTSLVQIHTGSEEPLYNIGVVSRMTKISMATLRAWERRYEFPES
                     KRTMGGHRLYSEKDIIRLRWVKERVDEGMQTAQAIHALRHQEQTGQAVLTEEDFRELT
                     PARIERMDAIDKGDREDRRSFSASVQERLLYALLHHNTQQADQLIADALAVLSPEVVI
                     LDIIRSVLNEVGELWQQDKISIANEHLATNYLRQRLLMWMVSGPPPRTQHSIVLACAP
                     DEWHEGSLLILGALLRRRLWPIVYLGQSVPLPDLASFVDDVNPSMVVLNAMTEETAKS
                     LMDWPKLLPDVVSKGRPVVGFGGRVFVEKPEWRLKIAGIYLGNNFEEGILTIEKLLRD
                     "
BASE COUNT         1271 a         1167 c         1058 g         1317 t
ORIGIN      
        1 tcgattactc cgctcttgga gaaatggccg ctctcagcac aatgcatatt tggaggatta
       61 cgccagtctg gtacttgccc ttatctccct ctatcaatcc gatcccaacc cctactggta
      121 tacctctgcg acgtccttgc tagacgagat gatccagcac ttctcagatc cttcaggcgg
      181 tttcttcgat acgcgcatcg atcatgaacc gctgattacc cgccccaaag atttacaaga
      241 caatgccacc ccctgtggaa actctctagc agcgatggcc ttgttacagc actcaaatta
      301 tagcggtcgg agtgatctgg ttgatattgc ggagacttcc cttcgctcaa ttattgatca
      361 atccagcaag tatccgacag catttgctta ctggctatgc gcagcagact atgttgccaa
      421 tccggtttct gaagtggcga taatcggatc tgctaaagat gagcgcactc aagcattagt
      481 gaatgtgctt tgggaaacct atcggcctcg cacgctgagc gcccaagcca atttaccaat
      541 ttctactgaa atgcctgaat tgcttgacaa tcgagagctc gtcgatgggc ttcctgcagc
      601 ctatgtctgc caaaactttg tctgcaattt gccggttacc acaccagatg aactacgcat
      661 tcaattgaag cgtgctgcat gatatcgtcc cgtaatcaag ctatacaact cgcaaaggaa
      721 aaaattaaat ccaatccggt gtatttggac accgaaacga caggatacgc cagggacagt
      781 gagattgttg aagtttgtgt attagacgat caaggggaag ttctgtttga gtcattggtc
      841 aagccaaccc agcttattcc aagagatgca gtcagaatac acggtatcac aaatgaaatg
      901 gtcagttctg ccccatcatg gatgtcagta tggcctaatc tagaaagggt gctctctggt
      961 catgtggtag gcatttacaa cgccgaattt gacctgcgat taatccagca gacacataac
     1021 aaataccgga tgaaatgggc ctcccgtgca gattttacgc cattttgtat tatgaaattg
     1081 tacgctcagt attacggaga gtggaatcat tctagggggt cttatcgctg gcattcacta
     1141 gaagacgctg gtcgtcagtg tcatatcccc ctgcccaatt cccaccgtgc tcgagatgat
     1201 tgcctgcttg cgcgcgcact gctgcacttc atggcttcct aatcaataaa taattctacc
     1261 ggacaatgat ctgagccagg gatatcatca tagatgacaa catcctgtac tcgaggcatc
     1321 aggttttccg aaaccagaaa gtaatcaagt ctccaacctg tatttcgctc acgcgctctg
     1381 ccaacataag tccaccaggt atactgaaca cgccctggat agagcctgcg gtaagcatcc
     1441 acaaaaccat gctctaagta gaggtcaatc caagcgcgtt cctctggcaa aaagcccgat
     1501 gttttggcat tttgttttgg attttttaga tcgatctctt tgtgcgcagt gttgaaatct
     1561 ccgcacaaaa ttattgcctg atttgctgca tggagactat cgcaaagatc cagaaggtgg
     1621 gcgtagaatt taagcttaaa tgctaaacgc ccatgatcgc gctgtccatt agggaaataa
     1681 atattgaaca gtaaaaactc ggggtatttt gagcatataa cccgcccctc tcgatcaaat
     1741 tcatctagac ctaaaccata gctaacatct aatgactcac ttctggcaaa tgtcgccacc
     1801 ccactatagc caagcttttc ggccggattc caaaaggcat gataatcttc aaacagctga
     1861 tgatcatcag tgttcaattg ctctggctta gactttatct cttggaagca taaaatatct
     1921 ggtgctcgta caagaattgc atccaaagcg cctttcctta gaacagagcg caatccatta
     1981 acattccaag tgataatatt catttttctt tccagtatca aattcatttt actccacagc
     2041 gcgaacaagg ggtaggaata cgatactgcg ctcatgttct tgaataaaat tgagcattat
     2101 tacagaattg tgctaaaatc ctggggatat gaagtcaatc atacaaccta ttaaaggcac
     2161 gcgcgatttt tacccagagt tgatgacaat ccgctcttgg ttatacgaaa ctgccagaga
     2221 gatttcggaa tcattcggtt accaggaata tgatgctccc tttcttgaac cgatcgagct
     2281 ctacgctgca aaatctggcg aggagctggt aaaagagcaa tctttcgtct ttccggatcg
     2341 gggtggagat ctgattgcat tacgcccaga gctaacccct tcactggcac gtatggtggc
     2401 acagcgacaa agacagctaa tttatcctgt gcgttggtgg tcctttggac cattttggag
     2461 atatgaacgc ccacaaaaag gtcgttcccg cgagtttttt cagtggaaca ttgacctttt
     2521 cgggataaac acccccgaag cagatgctga actggtggct atcgcctgtg catatatgca
     2581 ggcggtcgga ttaaccccac aagaagttgg cattttggtg aacaatcggc gcctagtgga
     2641 cgcagagtta gaagctctag gtatcccacc cgaaaacagg cctatagtct cacgctggat
     2701 cgaccggcgt ggcaagatgt ctccagatga ttggacatct tacggacggg atattggttt
     2761 atcgcgtgaa caactcgacc aaatgaaagc tcttctggct aacatagatc tctggaaaaa
     2821 gtcggaagag ctggtgcgct tctttgacgc cattgatgct ttgggatgtc gccaatacgt
     2881 ccgctatgat ccgaatattg tccgcgggct agactactac acaggtattg tctttgaagc
     2941 agtagatctc cagggagatg tacgccgctc aatattgggc ggtgggcggt atgataacct
     3001 gctagcggat gtgggtggtg atcccatgcc tggggttgga ttcgctatgg gggatatggt
     3061 aatcggtcta cttctagaaa agtacgggca tttacctgaa agtctagata cctctccggc
     3121 accagtctta gttactgtct tcgatgataa atcgctttta gcctctttca ctctggcggc
     3181 agagcttcgt cgcgcaggca tcaatgtgaa ttgctatcca gaggtagcaa agttaagcaa
     3241 gcagtttaaa tttgcagatc gagttggtat acggctggct gttgtcctcg gcccagaaga
     3301 gcagcagagc ggtatggtga ctatcaaaaa ccttcattct ggagaacaga tcaccatttc
     3361 gagatcctca gcatcatcta ctataagcca ttggcttgaa aagccagcct ctccatgata
     3421 gaatacggct tgctgtggtg cctgtagctc aatggcagag cacctgactg tggatcaggt
     3481 ggttgtgggt tcgaccccca tcaggcaccc tagctgaaag ggacttttct ttcgaaaagt
     3541 cccttttatt aaaccaataa tctttctttt tcaatccaca tcaaacgaat ttttcttatc
     3601 ccccaaagta cggccatctt aatctcgcaa tagcttctct atcgttaata taccttcctc
     3661 aaagttatta cccaagtaga tacccgcaat tttcaaacgc cactcaggtt tttctacaaa
     3721 aactcgaccg ccaaatccaa caacaggacg ccctttcgaa actacatcag gcaacagctt
     3781 aggccaatcc attaagcttt ttgctgtttc ttccgtcatt gcgtttaata cgaccatcga
     3841 aggattcaca tcatctacga agctggctag atctggcaac ggaaccgact gaccgaggta
     3901 cactattggc cataacctcc ttctcaggag agcacccaga atgagcaagc tgccttcatg
     3961 ccattcgtca ggcgcgcagg ccaggacaat agaatgctgg gtccgggggg gaggtccgct
     4021 aaccatccac attaacaagc gttggcgtaa ataatttgtt gctagatgct cgttagctat
     4081 gctgatttta tcttgctgcc acagctcgcc aacttcattc aaaactgacc tgatgatatc
     4141 cagtatgaca acttcgggag ataaaacggc tagcgcatca gcaattaatt ggtctgcctg
     4201 ttgggtatta tgatgcaata acgcatacag caacctctct tggacgctcg cagaaaagct
     4261 tcgtctatct tctctgtcac ccttatctat ggcatccata cgctcaattc gggctggcgt
     4321 cagttctctg aagtcttcct ctgtgagaac tgcctggcct gtctgttctt ggtggcgtag
     4381 cgcatggatt gcttgagcgg tttgcatccc ttcgtcgacg cgctccttta cccatcgtaa
     4441 gcgaatgata tccttctctg agtacaagcg atgcccgccc attgtccttt tggattctgg
     4501 aaattcgtac ctgcgttccc aggcacgcaa ggtcgccatt gaaatttttg tcattcgcga
     4561 gacaacaccg atattgtata aaggttcttc actccccgtg tgaatctgca caagtgaagt
     4621 cataaacact cctgtttctt gtccattttt tatagattta gtttatcatc caaagtcttt
     4681 tttgtcaata ttattcgaca attgctatac aatatattga catttatcct ggcaggggtt
     4741 ataatgttag aaattagaaa ccgagaatat acaaatagaa aatactagga ggttggtatg
     4801 cgggtaatca tta
//
LOCUS       DBNGSX010000474         2552 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_601679, whole genome shotgun sequence.
ACCESSION   DBNGSX010000474 DBNGSX010000000
VERSION     DBNGSX010000474.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_601679"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="AC16CB_14020"
     CDS             complement(<1..484)
                     /locus_tag="AC16CB_14020"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3343633.1"
                     /translation="MKIRLGKLFQWIGILLIVLLGLALLALLALYYTSNQKLNKTYEV
                     SFEPLEISMDPETIERGRRIVTTRGGCIECHGGNFAGDVIDQGFLMARLVVPNLTPGK
                     GGVGASFTDDDWVRAIRYGIDSDGTSVIIMPSDYFNVLSDADLAAIIAYLRSLPPVDT
                     N"
     gene            complement(541..1242)
                     /locus_tag="AC16CB_14025"
     CDS             complement(541..1242)
                     /locus_tag="AC16CB_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343634.1"
                     /translation="MLGHNLRQIAVRSALVTALFLFGGFLLGFAAAIGFSQLPMHVPD
                     SLQMLVAMAIILSILFVAGAAWGRAMARLSGFAETRRMAKAGAVSFGPALVLSALVLS
                     SLEVAIIERGGGTSLPIHVVFTILFVPAVFIVAGVGGLALGIAQGDWRFAWRCALSSG
                     LAGSAAFLLVNLGMDQLGWRVGAPGAAERATMLTVMMAGSLGAALAGGGAIGGMLGRN
                     ALHGVSTALEVDQYN"
     gene            complement(1317..2225)
                     /locus_tag="AC16CB_14030"
     CDS             complement(1317..2225)
                     /locus_tag="AC16CB_14030"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3343635.1"
                     /translation="MKKVLKWIGIVLGGLIGLLVIAVIGLALYASSKFKPTYANRPLY
                     QITADTSPEGVARGKYLMEEAMLCAEACHSPEGKPFQGYIEEISEGPIQVLFAVPNLT
                     PDVETGLGSWSDAQIARAIREGVDKDGVGLVVMPAYNYKALSDADVAAIIGYLRNLEP
                     VHHEIPPISANIVAKVMMAMGAFGPSSVGEPITAPKDTPPAGTPEYGQYMLALGACSD
                     CHKQNLAGGPIPFAEEGMPWSANLTPAGELANWSEADFIAAVSEGRHPGGGNLHDEMP
                     RYKMNNEDLKAIFMYLKTIPPAQPEK"
BASE COUNT          642 a          736 c          617 g          557 t
ORIGIN      
        1 aatttgtgtc tacgggtggc aaactgcgca ggtaggcaat gatcgcagcc aggtcagcgt
       61 cgctgaggac attgaaataa tcggagggca tgatgattac agacgtgcca tcggaatcta
      121 taccgtaacg gatggcgcgt acccaatcgt catcggtaaa gctcgcgcca acaccaccct
      181 tgccaggggt taagttgggg acaaccagcc tggccattaa aaagccttga tcgatgacat
      241 ccccagcgaa attacccccg tgacattcga tgcacccacc tcgagtcgtc acaatacgac
      301 gcccgcgctc gatggtctct ggatccattg aaatttccaa cggttcaaac gaaacctcgt
      361 aggtcttgtt cagtttttga ttgctggtgt agtacagggc aagcaaggcc agcaaggcta
      421 atcccaataa gacgattaac aggattccga tccattgaaa taatttccca agcctgattt
      481 tcacttttca cctcatagaa tagttttcta ccaccatcta gttgttatta atcgtaccgg
      541 ttaattatac tgatcaactt caagcgcggt ggaaacaccg tggagagcat tcctacccag
      601 catgcctccg atggccccac cgcccgccag cgcggcgcca agactgccag ccatcatcac
      661 cgtcagcata gtagcccgct ccgccgcgcc gggtgcgccc actcgccagc cgagctggtc
      721 catccccagg tttactagca agaaggccgc gcttccggca agtccgctgc tcaatgcgca
      781 gcgccaggca aatcgccaat ccccctgggc aatacccaaa gccaaacctc ccacgcccgc
      841 tacaatgaag accgccggca caaagaggat ggtgaagaca acatggattg gtagcgatgt
      901 gccgcctccg cgctcgatga tggcgacttc cagggaggac aagaccaatg cactcaagac
      961 cagcgccgga ccaaaactca ccgcacccgc ctttgccata cgccgcgtct cagcgaatcc
     1021 actgaggcgc gccatggctc gaccccaggc tgcacctgcg acaaacagga tgctcagtat
     1081 aatcgccata gcgacaagca tctgcagcga gtcgggcacg tgcataggta gctggctaaa
     1141 ccctatcgca gccgcaaagc ccaatagaaa accgccaaat aaaaacagcg cagtaaccaa
     1201 ggcactgcgg acggcgatct ggcgcaaatt atgccccaac atttttacct cttgtctcct
     1261 gtagttgtcc cagaagggtt ggggaagcaa atccccaacc ccctggtagc tgttaactac
     1321 ttctctggct gcgctggcgg gatagttttt agatacatga aaatggcttt caggtcttca
     1381 ttgttcattt tatagcgcgg catctcatca tgcagattgc ccccccctgg atggcgccct
     1441 tcactcacag cggcaataaa atcagcctcg ctccaattgg caagctcgcc cgctggggtc
     1501 agattagcag accatggcat accttcttcg gcaaagggaa taggaccacc tgccaaattc
     1561 tgtttgtggc agtctgagca ggcgcccaag gcaagcatat attggccgta ttcaggcgtg
     1621 ccagccggag gggtatcctt tggggctgta atcggctcgc caactgaact ggggccaaaa
     1681 gcgcccatcg ccatcatcac cttggcgaca atgttggcgg agataggtgg gatctcgtgg
     1741 tgcaccggct ccaaattgcg caagtaaccg ataatcgccg ccacgtcagc atcgctcaaa
     1801 gccttgtagt tgtaagctgg catcaccacc agcccaacac cgtctttgtc cacaccttcg
     1861 cggatggcgc gggcaatctg ggcgtcactc caactcccaa gaccggtttc cacatcgggg
     1921 gtcaggttgg gcacggcaaa taggacctga atcggtccct cgctgatctc ctcgatgtat
     1981 ccttgaaaag gcttgccttc aggggagtgg caagcctcgg cgcacagcat ggcttcttcc
     2041 atcagatatt taccccgcgc cacaccctct gggctggtat cggctgtgat ctgatagaga
     2101 ggacgattgg cataggtggg cttgaacttc gacgaagcat atagtgccag gccgatgact
     2161 gcgatcacca gcaggccgat cagaccgccc aaaacaatac caatccattt caatactttt
     2221 ttcatcttta tttcctccta aaaaatgaga tactcggcgg agacacacct gtcaccgtaa
     2281 ccgctggaaa gctattgatc aatcagtttc caacaggatg atgtgaattt cacctctact
     2341 catagataaa tggatatttt tcatcttgta tggcttattt taacatcctt tcgtccctaa
     2401 ctcgttacgg gaagcgttac gggaggctat atttatgcag taaaatctaa gcggaataag
     2461 gccatttgtc ccagaatatc aactacaata ttctggtttc aataccccat cactgggtgg
     2521 gcacgagaac atgaagggct gaaacagatt ga
//
LOCUS       DBNGSX010000475         6374 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_740363, whole genome shotgun sequence.
ACCESSION   DBNGSX010000475 DBNGSX010000000
VERSION     DBNGSX010000475.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6374)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_740363"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2020)
                     /locus_tag="AC16CB_14035"
     CDS             complement(<1..2020)
                     /locus_tag="AC16CB_14035"
                     /inference="COORDINATES: protein motif:HMM:NF013491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR3343636.1"
                     /translation="MTLPPPSADRTLNIYLSLAQFPILSGKIRARMRRELFERGVISL
                     QAFEAEVREKAIRSQSMEGVNNPFSEEPADIWELRLVRVRDHLTDFYFAYNLPYELFE
                     RIVRQVLTERGTPENEVTVAFNPELAPQSMLFEQADAIEKLPADDRARMEARLREIKV
                     VLIRTMISDHLAYVNIAKEWFTIADLKKIRERKIGQGKIGGKSAGMLLAARILNEAGD
                     DDIRASLRYPESYFIGADLTYGFMSLNSLMHWGDQKYKTEEQIRAEFPELQEEFLAGE
                     FPEDILDSLRGLLEQVGGQPIIVRSSSLLEDSFGTSFAGKYESHFCPNQGSPEENLES
                     LTHAIKRIYASIFLPDALLYRRAKGLQDYDERMAILIQMVVGERFGRYFLPQASGVAF
                     SRNLYRWSPQIRREAGFSRLVWGLGTRAVDRVGNDFPRLVALSHPLLHPQDNTKDIRG
                     YSQRYVDLIDLQENMFKTLPVQEVLQARYPALRYLAQLDQGGYLAPLRSMTMESKTER
                     LVLTFDELFRRTPLAERLSTIVQLLETHYRSPVDTEFAAQIVDPNALQPEVIINLLQC
                     RPQSHLKDSEVHLPKHLPDEDIIFSTRRMAPRGHISEIRYVVFVTPEGYFSLPTQAAR
                     AEMSQVIRQLNRELAEEQFILLGPGRWGTSNPDLGIHIAYGDIYHTRAL"
     gene            complement(2103..3236)
                     /gene="dnaN"
                     /locus_tag="AC16CB_14040"
     CDS             complement(2103..3236)
                     /gene="dnaN"
                     /locus_tag="AC16CB_14040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR3343637.1"
                     /translation="MKVSVLQENLAHGLSIVSRAVSARSTLPVLSNVLVATDEGRLRL
                     SATNLELGITCWIGAKITEEGSTTVPARTFTELVSTLSDKQVDMDLNVRTQTLNVHCG
                     ASNTDLKCIDSQEFPPMPAPDLAHGIQINVADLKEMIQQVAFAASTDEARPILTGVLV
                     DVEDNQITLSAADGFRLSIRHAELSSPVPGAMRAVIPARALTELARISADGDQVITMV
                     MPENRGQVIFGMENAELVSQLIEGTVPDLQQLVPASYNTRAVLSTSQFLKACKQAEIF
                     AREGSHIARINILNGGEQEPGQVEISGQSDETGFNQTVVDATIEGDPQLIAFNVRFLR
                     EVLDVIKTPNVALETTSGNSPGVIRPVGEDNFLHVIMPMHLGS"
     gene            3420..4847
                     /locus_tag="AC16CB_14045"
     CDS             3420..4847
                     /locus_tag="AC16CB_14045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR3343638.1"
                     /translation="MPHYDWTDDGYFHTEQRDLTQIRRVLIITMLLNFLAMAIKIVTG
                     LLTGALSVLADGLDSLFDGLSNVVGLAGLYAAAKPPDADHPYGHRKFETLAALSISFL
                     LFLTCWQLLQTAWQRLGQDHLPDINLWTAVVMLVSMLIQAITSLYELRKGRKLQSEIL
                     VADALHTRASILVSLSVLIGLGFVRLGYPQADALLAAFVALMIAKIGVDILRETLPVL
                     VDEAAIDPAQIAEVVSSVSGVESFHRVRSRGADGSALVDLHVRVSPNVALQEADAIAK
                     EVRRRLLELDKVTDVTVHVEAQRYSQTDGADLFATLKHTASELGLVIHESWAHRLDGK
                     LVVEVHVGVDPALTLGEAHDLVDRLEVELRRRSPEIEVVHTHIEMADLQVGEGQRAPL
                     ELEQRVRQQVEQSVAEIAGLSRPHNIILRRNRPDSEYYHIALECHIAPDTPVGDAHQL
                     SHLLEGEIKRRLPEVVEVFVHLEPG"
     gene            4856..>6374
                     /gene="polX"
                     /locus_tag="AC16CB_14050"
     CDS             4856..>6374
                     /gene="polX"
                     /locus_tag="AC16CB_14050"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HIR3343639.1"
                     /translation="MSANKKNYTNADLAGIFQTIADMLEIKGEVIYKILAYRKAAESL
                     TNLGRDVSDVWREGKLTEIPGVGKAIAEKIDELLTTGRLEFFEKLKSEVPVSLVELLQ
                     VPDLGPKKIALFWRELDITDLPGLEAAARAGKLRGLPGMGEKSEAKIIAGIESLSRRT
                     TRIPLGRAWPFAQQLIKSLGDIPGVVAVEAAGSLRRMRSTVGDIDLLVAASDSNAVMQ
                     AFVEHPEVARVLGHGETKSSVEYHHGLRAQLWVHPPQRFGTALQYATGSKDHNVRLRE
                     LALKRGLSLSEHALTRKDGSEILCATEDEVYAALGLPWIPPELREDRGEVAAAMAGEL
                     PRLIHLDDVRAELHTHSTWSDGKLSIQAMAEEARQRGMKVLAITDHTASLGVAGGLSV
                     DEIRQQRGEIDAAQGALGDTIRLLQGAEVEIRADGSLDYPDEALAELDIVIAALHTGL
                     RQTREQVTSRTLSAIHNPHVDVIAHPTGRMIPDREGADLDMDAVLSAAAETGTALE"
BASE COUNT         1342 a         1912 c         1865 g         1255 t
ORIGIN      
        1 ccagggcgcg cgtatggtaa atgtctccgt aggcaatgtg gatgcccagg tcagggttcg
       61 aggtacccca gcgtcccggc ccgagcagga tgaattgctc ctcagccagt tctcgattta
      121 gctggcggat gacttggctc atctcagccc gcgctgcctg cgtgggcagg ctgaaataac
      181 cctcaggggt gacgaagacc acataacgga tttcggagat atgaccccgc ggagccatgc
      241 gccgggtgga gaagatgatg tcttcgtctg ggagatgctt tggcaggtgc acctcactat
      301 ctttgaggtg gctctgtgga cggcactgca gcaagttgat tatcacctct ggttggagcg
      361 cattcggatc gacaatttgg gcagcgaatt cagtgtcaac aggcgagcga tagtgagttt
      421 ccaagagttg cacaatagtg ctcaggcgtt ctgccaatgg cgtgcggcga aacagctcat
      481 caaatgtcag caccagccgc tcggtcttgg actccatcgt catgctacgc agcggcgcaa
      541 gatagccgcc ctgatctaac tgagccaaat agcgcaacgc ggggtagcgc gcttgcagga
      601 cctcctggac aggcagcgtt ttaaacatgt tctcctgcaa atcaatcagg tcaacatacc
      661 tctgggaata gccgcggata tccttggtgt tgtcttgtgg gtgtagaagc gggtggctga
      721 gggcaaccag ccgggggaag tcgttgccca cccggtccac agcccgcgta cccaagcccc
      781 acactaaccg cgagaaacca gcctcgcggc ggatctgcgg cgaccagcga tacaggttgc
      841 ggctaaatgc cacgccagac gcttgcggca ggaaataacg cccgaaacgt tcccccacca
      901 ccatctggat caggatcgcc atgcgctcgt cgtaatcctg caacccctta gcgcggcgat
      961 agagcagggc atcgggcaag aatatgctgg cataaatccg cttgatggcg tgcgtaagac
     1021 tctctagatt ctcctctggt gacccctggt tgggacagaa atggctctca tacttaccgg
     1081 cgaaagacgt tccaaagcta tcttccagca ggctggatga tcgcacaatg attggctgtc
     1141 ccccaacctg ctccaacaaa ccccgtaaac tgtccaggat atcctcgggg aactccccgg
     1201 ccaggaactc ttcttggagc tcgggaaact cggcccggat ctgctcctca gtcttatact
     1261 tctggtcccc ccaatgcatt agcgaattga gggacataaa gccataggtc aggtcagcgc
     1321 cgataaaata cgactcggga taacgcaggc tggcgcggat atcatcgtct ccggcctcgt
     1381 tcaagatacg ggcagccagc agcataccgg cggatttccc cccgatcttc ccctgaccaa
     1441 ttttccgctc gcggattttc tttaggtcgg cgatggtgaa ccactctttg gcgatgttga
     1501 catacgccag gtgatcgctg atcatggtgc ggataagcac aaccttgatc tctcgcaggc
     1561 gggcttccat gcgagcgcga tcatccgcag gtagcttctc gatggcatca gcctgctcga
     1621 aaagcatgct ctggggtgcc agttctgggt tgaaagctac tgtcacctca ttctccgggg
     1681 tgccgcgttc ggtcagcacc tggcgcacga tgcgctcgaa cagctcatac ggtaggttgt
     1741 atgcaaagta gaaatctgtc aggtggtcgc gtacccgcac caagcgcagt tcccagatgt
     1801 cagcgggttc ctccgaaaat ggattgttca ccccttccat cgactgggag cggatcgcct
     1861 tttcacgcac ttcagcctcg aaggcttgca ggctgatcac gccgcgctca aacaattcgc
     1921 gccgcatgcg ggcgcgaatc ttgccgctca agatgggaaa ttgagccaga gaaagataaa
     1981 tattcagcgt gcggtctgca gacggcgggg gcagggtcat acaggttgca gaatcaatgg
     2041 ttgaaggctg aaggtttgct ctcgtggata gcctacaacc ttcaacccgc aaccgtccag
     2101 ggttagctcc ctaaatgcat tggcatgatc acgtggagga aattatcttc tcctaccgga
     2161 cggatgacgc cgggactgtt cccagatgtg gtctctaagg ctacattggg tgtcttgatc
     2221 acatccagca cctcacgcag gaagcgcaca ttaaatgcga tcaactgcgg gtctccctca
     2281 atcgttgcat cgaccactgt ctggttgaaa ccagtctcat ccgactgtcc cgatatctcc
     2341 acctgcccgg gttcctgttc gccaccattg agaatgttga tgcgagcgat atgcgacccc
     2401 tcgcgagcga agatctcagc ttgcttgcaa gccttaagga actgggaagt ggagagcacg
     2461 gcgcgggtgt tgtaactggc tgggaccaac tgctgaaggt ctgggactgt gccctcaatc
     2521 agttgggaga caagctccgc attctccatg ccgaagatca cctgcccgcg gttctctggc
     2581 atgaccatgg ttatcacctg atcgccatct gccgagatgc gcgccagttc ggtcaatgcc
     2641 cgcgctggga tgacggcgcg catagcgccc ggcactggcg atgaaagctc agcatggcgg
     2701 atagaaagac gaaacccatc cgctgccgaa agcgtgattt gattgtcttc cacgtccacc
     2761 agcacgccgg tgaggatagg ccgcgcttcg tcggtcgagg cagcaaaggc aacttgctgg
     2821 atcatctcct tcaagtcagc cacattgatc tggatgccat gcgccaggtc gggtgcgggc
     2881 ataggcggaa actcttgcga atcgatgcac ttcagatcgg tattcgaggc gccacaatgc
     2941 acattcaaag tttgcgtgcg cacattgagg tccatatcaa cctgtttatc cgagagcgtg
     3001 ctgaccagct cggtgaaggt gcgcgccggc acagtggtgg agccctcttc ggtgatcttg
     3061 gcaccgatcc aacaggtaat gcccaactct agattggtag ccgacaggcg caggcgaccc
     3121 tcgtccgttg ccaccagcac attcgacaac accggcaggg tgctgcgagc agagacggcg
     3181 cgcgagacga tactcagtcc atgagctaaa ttttcctgaa gaacagatac tttcataaga
     3241 acgactctcc aattaaggtt agtatgagta tacaacgcct aagatatttt gtgaagaaat
     3301 tttagaaatt gggtcaaggc aatcgcattg gttttgcctt cacaccgaaa aaatggacgc
     3361 cgaataaccg tatagaggct gtgggcaacg atgaaaaaat ggtatgattc gtccacacca
     3421 tgcctcatta cgactggact gacgacggat atttccacac cgaacagcgc gacttgaccc
     3481 aaatccgccg cgtgctgatc atcaccatgc tgctcaactt cctggcgatg gcgatcaaga
     3541 tcgtcaccgg cctcctgacc ggcgcgctga gtgtgctcgc cgacgggcta gacagcctgt
     3601 tcgacggcct ttccaatgtg gtcggcctgg ctgggctgta cgccgcggcg aaacctcctg
     3661 atgctgacca tccttacggg catcgcaaat ttgagacgct tgccgcatta agcatctcct
     3721 tcctgctctt cctcacctgc tggcagttgc tgcaaaccgc ctggcaacgc ctgggacagg
     3781 atcacctccc agacatcaac ctgtggaccg cggttgtcat gctagtatcc atgctgatcc
     3841 aggctatcac cagcctgtac gagctgcgca agggacgaaa gcttcagagc gagatactgg
     3901 tagccgatgc cctgcacacg cgtgccagca tcctggtctc cctgtctgtg ctgattggct
     3961 tgggattcgt ccgcctgggc tacccgcaag ccgatgcgct attagcggcc ttcgtagccc
     4021 tgatgatcgc caagatcggc gtggacatcc tacgtgagac cctgccggtg ctggtggatg
     4081 aggccgccat tgacccggcc cagattgccg aggtggtgag ctccgtcagc ggggtggaat
     4141 ccttccaccg cgtgcgcagc cgtggcgccg atggtagcgc tctggtagac ttgcacgtgc
     4201 gtgtctctcc caatgtagcc ctccaggagg ccgatgccat cgccaaagaa gtccgccgcc
     4261 gcctgctgga actggataaa gtcaccgatg taactgtcca cgttgaagcg caacgctatt
     4321 cccaaaccga cggcgccgac ctattcgcca ccctcaagca caccgccagc gagctggggc
     4381 tggtcatcca cgagtcctgg gcgcaccgcc tggatggcaa gctggttgtg gaggtacatg
     4441 ttggagtgga cccggctctg accttgggtg aagctcacga tttggtcgat cgcctggagg
     4501 ttgagctacg ccggcgctcc cccgagatcg aagtggtgca cactcatatc gagatggccg
     4561 atttgcaggt gggtgaaggc cagcgcgccc cgctggagct ggagcagcgt gtgcggcagc
     4621 aggtagagca gagcgtcgct gagatcgccg ggctgagccg cccgcacaac atcatcctgc
     4681 gccgcaaccg tccggacagc gagtattacc acatcgctct ggaatgccat atcgctcccg
     4741 acacacccgt aggagatgcc caccagttgt cgcacttgct ggaaggtgaa attaagcgcc
     4801 gcctgccaga ggtggtggaa gtgtttgtgc atttggagcc agggtaaatt tcaccatgtc
     4861 tgccaacaaa aaaaactata ccaatgccga cctggctgga atctttcaga ccatcgccga
     4921 catgctggag atcaaaggcg aggtgatcta caagattctg gcctaccgca aagctgccga
     4981 gagcctcacc aacctgggtc gggacgtgag cgatgtgtgg cgcgagggta aattgaccga
     5041 gatccccggc gtgggcaagg ccattgcaga gaagatcgac gagctgctta ccaccgggcg
     5101 gttggagttc ttcgagaagc taaaaagcga ggtgcctgta agcctggtgg agctgctcca
     5161 ggtgcccgac ctgggtccta agaagatcgc cctgttctgg cgcgagctgg atatcaccga
     5221 tctgcccggg ctggaggcgg cggcgcgagc cggcaagctg cgcggcctgc caggcatggg
     5281 tgagaagtcg gaggccaaga tcatcgccgg catcgaatcc ctgtcgcgcc gcaccacgcg
     5341 catccccttg ggccgcgcct ggcctttcgc ccagcagctg atcaaatcgc ttggagacat
     5401 accaggcgtg gtggcggttg aagccgccgg cagcctgcgc cgcatgcgct ccacggtggg
     5461 cgatatcgac ctactggttg ccgccagtga ttcaaatgct gtcatgcaag ccttcgtgga
     5521 acacccggaa gtagcccgcg tcctgggtca cggcgagacg aaatccagcg tggaatacca
     5581 tcacgggctg cgcgcccagc tctgggtgca ccccccgcag cgcttcggca ccgccctgca
     5641 gtacgccacc ggctccaaag accacaacgt gcgcctgcgc gagctggctt tgaagcgcgg
     5701 tctctcgctc tccgagcacg ccctgacccg caaggatggc tccgaaatcc tgtgcgccac
     5761 cgaagacgaa gtctatgccg ccctggggtt gccctggatc ccccccgagc tgcgcgagga
     5821 ccgcggcgag gtggcggctg ctatggcagg cgagctgccc aggctgatcc acctcgacga
     5881 cgtgcgcgcc gagctgcaca cccattccac ctggagcgat ggcaagctca gcatccaggc
     5941 catggccgag gaagccaggc agcgcggcat gaaggtgctg gccatcaccg accataccgc
     6001 cagcctgggc gtggcgggtg ggctttcggt ggacgagatc cgccagcagc gcggcgagat
     6061 cgatgccgcc cagggtgcgc tgggcgacac catccgcctg ttacaaggag ccgaggtgga
     6121 gatacgcgcc gacggcagcc tggattaccc cgatgaggcc ctggccgagc tggatatcgt
     6181 catcgccgcc ctgcacaccg gcctgcgcca gacgcgggag caggtcacct cgcgcacgct
     6241 gagcgctatc cacaacccgc acgtggatgt gatcgcccac cccaccgggc gcatgatccc
     6301 cgaccgcgaa ggagccgacc tggacatgga cgccgtgctg tctgccgccg ccgagaccgg
     6361 caccgccctg gaga
//
LOCUS       DBNGSX010000476        10474 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1294818, whole genome shotgun sequence.
ACCESSION   DBNGSX010000476 DBNGSX010000000
VERSION     DBNGSX010000476.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10474)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10474)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10474
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1294818"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="AC16CB_14055"
     CDS             <1..224
                     /locus_tag="AC16CB_14055"
                     /inference="COORDINATES: protein motif:HMM:NF018964.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoprotein intramolecular transacylase Lit"
                     /protein_id="HIR3343640.1"
                     /translation="FLLAGIILFVMLAFGVIFVAFHNVFFEAGTWQFLFSDTLIRLFP
                     ERFWRDTFIAIGVLAGGTGLALGYFLREK"
     gene            complement(268..1014)
                     /gene="gpmA"
                     /locus_tag="AC16CB_14060"
     CDS             complement(268..1014)
                     /gene="gpmA"
                     /locus_tag="AC16CB_14060"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HIR3343641.1"
                     /translation="MHKLVLIRHGQSTWNLENRFTGWTDVGLTPQGETEAHTAAKLLA
                     EEGFTFDLAYTSVLKRAIKTLWIVLEDMNLEWIPVYRNWRLNERHYGALQGLNKAEMA
                     VEYGEEQVKIWRRSYDTPPPALDWDDSRHPRFDARYADLTKDDLPACESLKDTVARML
                     PYWHETIAPAVRSGQRVVISAHGNSLRALVKYLDDISEKEIVELNIPTGIPLIYELDD
                     DLKPLKSYYLGDAEAIRLAAEAVAKQGQAR"
     gene            complement(1196..2296)
                     /locus_tag="AC16CB_14065"
     CDS             complement(1196..2296)
                     /locus_tag="AC16CB_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3343642.1"
                     /translation="MASVTYEHVYKRFGDVTAVNDLDIQIADKEFLVLVGPSGCGKST
                     ALRLLAGLEDISEGQILIGDQVVNEVAPKDRDIAMVFQSYALYPHMSVYDNMAFGLKL
                     RKMSKAEIDRRVKEAADILGIGDLLNRKPRQLSGGQRQRVAVGRAIVREPKVFLFDEP
                     LSNLDAKLRVETRANISKLHIQLQTTFIYVTHDQTEAMTMATRIAVINKGLLQQIDTP
                     QTLYDTPANLFVAGFIGSPAMNFFPAKLVKSDGNLIVDATAFQVQIPTERMDVYKPHE
                     GKSIIFGIRPEDIHNPNFAPPGIQTQPVNAKVDVTELMGNEIFVYLKSGNNNFVARVD
                     PRSRYSINDDVQVFFNMENIHIFDKDSEQAIR"
     gene            2435..5020
                     /gene="mutS"
                     /locus_tag="AC16CB_14070"
     CDS             2435..5020
                     /gene="mutS"
                     /locus_tag="AC16CB_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIR3343643.1"
                     /translation="MSPKDDISPIRKQYLEIKRQYPNAILFFRLGDFYETFDQDAEVT
                     SRELDIVLTSRNVAKGTRVPMAGIPHHAAENYLARLIERGYHVAICEQVGDQPIKGLF
                     PRQVMRVVTPGTVIEPGLLPGDANNYLACVVISEDEDVRRAGVAYADITTGEFATTEL
                     QEGDLQTSVRAELIRLMPAEILHPDSYKTGNGLPGHLTAWPAWRFELGRCQEALLKHF
                     ETATLDGFGLRGLPLAVRAAGAILQYLGETQAASLKQLSRLSVYSLLDFMTLDAATRR
                     NLELTETIRGSSSKGSLLGVLDHTITPMGKRLIRQWVSKPLLDVEKICQRQDQVQFFY
                     TESMTRTELRSALKPLGDLERLTNRVVSGHAQPRDLVAMRNTLRQLPGLRGMFPSTDS
                     QMDPVLRDLHTCSDELGLLEAAIEDDSPATLQNIGVIRSGYSAELDGVVERSRHARQW
                     IAGLEGVERQRTGIKSLKVGYNKVFGYYIEVTKANVDMVPAEYIRKQTLVNAERYITP
                     ELKEYETLVLNAEERIREIEGRLFHEVCAQLGGSATKLLAVAGALAQLDVLAALAEAA
                     ILGNYVRPDVVVDDVLEINDGRHPVVEQMLVGERFVPNDVMFEVSERVRIITGPNMSG
                     KSTLIRQVALIVLMAQMGSFIPADKARIGLVDRIFTRIGAQDEIHAGQSTFMVEMVET
                     ANILHHATPRSLLILDEIGRGTSTYDGVSIAWAVVEYIHNHPNLRAKTLFATHYHELT
                     QLADLLPGVRNYNVAVSEAEGKVVFLHKIIPGGADRSYGIHVGQLAGLPKPVIQRANE
                     IMRSLEASSGKAVRINPMAAKQMALFPETNPLLDELEDLDVNGLSPIEALNKLYEWQQ
                     KYLNK"
     gene            5025..5690
                     /locus_tag="AC16CB_14075"
     CDS             5025..5690
                     /locus_tag="AC16CB_14075"
                     /inference="COORDINATES: protein motif:HMM:NF024067.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HIR3343644.1"
                     /translation="MRESRKPNRPLSPQSNDRTMLLMARVDEMRVELRAQDPFKLMQR
                     VSGRFVQTQSGKGELRLALWGREIFVTFPDFIAFEGSSQQKLHAGLQALVIYYLYTTD
                     GAPIADRFISFSELPDGKFYAQAFQGYTGQELQHHFGDDRQRFERAAQNIGGRHYPLG
                     DASFTFQLLPRVVLLVVFWCGDEDFPSSYQVLFDASISHHLPTDVCAVAGSMLTRQLI
                     ESE"
     gene            5709..6512
                     /locus_tag="AC16CB_14080"
     CDS             5709..6512
                     /locus_tag="AC16CB_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343645.1"
                     /translation="MRNYLRLILFVLLFAGVLNTSCAGNQGISNPETATAQAGRIIGL
                     ATQMGGYAQATQMAENLQVTATAAARQLLLDEVGQWRLLLSDSYDEDLNDWSTGSDED
                     PELGSISWVIEDGKYRWQGLATNGFVWWVIPDIESVSDFYLSVSARQEAQPEVGEYGL
                     VFRQSGDVDYYLFEISDLGLYSLYLHTDSGWESLIDWTAHPSINPGATNRLAVIATGE
                     HFDLFINDTFVAEYSDDRLPEGKAGLLVGLSNPGDAANWEFDDFEFRAP"
     gene            6659..7720
                     /locus_tag="AC16CB_14085"
     CDS             6659..7720
                     /locus_tag="AC16CB_14085"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF019121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR3343646.1"
                     /translation="MKKFRVALLANLKVNAPKFEGMPEDQWDDLDSEKTINALVNAIR
                     AGGNSCEFLEGNLSLVDSLQKFKPDICFNICEGHFGDAREAHVPALLEMLRIPYTGSR
                     VLTLALALDKPMTKRVLAYHDLPTPPFQTFERVDEPLSTDMNYPLFVKPSREGTGMGV
                     SGRSIVRDEDELREQVDFILKRYKQPALVEGYIEGREVTLGVVGNLIGPVARRLPEDD
                     EAPRIQSGLRFFPPMEVNLKPFEDSDVVYSGRLKVDMADQLDYLCPAPLDSDMVDELN
                     WLTAAVFRVTGAMDVSRVDFRLDVHDNWKPYILEINPLPGLSPGISDLVIEAAAEGVD
                     HTYLVNMILDTALERYGMI"
     gene            complement(7717..8412)
                     /locus_tag="AC16CB_14090"
     CDS             complement(7717..8412)
                     /locus_tag="AC16CB_14090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3343647.1"
                     /translation="MSEIAVIIPALNEAGNLFRLIGEVRSTVNADIIVVDNGSTDETA
                     DEAIRAGAQLIQEPRRGYGYACAAGVAASEGSEILVFLDGDFSSLPSEIPALVNPIQS
                     GEADLVQGSRVLGHIAAGAMPFQQRFGNWIASRLMNFLYGSRITDLGPFRAIRKSLLS
                     TLNMREMTYGWPTEMTVKAQRRGARIKEVPVSFHPRQAGHSKISSTLRGTILAGWYIM
                     GVTLRYAWGSWDD"
     gene            complement(8409..9089)
                     /locus_tag="AC16CB_14095"
     CDS             complement(8409..9089)
                     /locus_tag="AC16CB_14095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR3343648.1"
                     /translation="MSLPLHALLVMAKRPSPGRTKTRLSPPLSPKAAGGLYECFLLDT
                     LDLVRQVPDVHKVIAFTPKSASGYFSKLAADFELIPQTGSNLGSRLDNALSYYLLNGF
                     TQVVIMNSDGPTLPVEYLIKAHKALAQVDVVLGPSEDGGYYLIGLKKPAPRLLRDVHM
                     STPRVTSDTLRIADSLGLRVHLLPGWYDIDDVETLDRLLVDLEQSPRLIAPQTRRYLK
                     QINMLSTR"
     gene            complement(9170..9931)
                     /locus_tag="AC16CB_14100"
     CDS             complement(9170..9931)
                     /locus_tag="AC16CB_14100"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HIR3343649.1"
                     /translation="MNNSFYIPESALIIVAHPDDIEFSCAGTLARWAQAGTRLCYVLC
                     TSGDVGIAEPGMTKAQAIAIREAEQLQAAEIVGTKDVVFLREPDGMLVPTIELRKKLV
                     REIRRFRPEVVVCGDPTIVWVGNDYINHPDHRAAASAALDAIFPAAGQPNLFEELEEE
                     GIHAHKPRKVYVTGWVQHELYVNITETIDIKIAALRAHKSQMQDWDPEPRIKEWAAER
                     AKGKEMVYAETFRVVTLVEDEEWQKTNGRVLPDKS"
     gene            10131..10379
                     /locus_tag="AC16CB_14105"
     CDS             10131..10379
                     /locus_tag="AC16CB_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343650.1"
                     /translation="MDRYLIETPHTDEDCQRLIDNVYAMGYIYHFDWGCDAGVHCGWA
                     IIEAEDEEQARLAVPSLVRNKARVIKLKKYTPETLDDH"
BASE COUNT         2470 a         2559 c         2777 g         2668 t
ORIGIN      
        1 tgtttctgct tgcaggaatt atcctttttg tgatgttggc gttcggggtg atcttcgtcg
       61 catttcacaa tgtcttcttt gaggcgggca catggcaatt cctgtttagc gataccctga
      121 tccgcctgtt cccagagcga ttttggcggg atacatttat cgcaatcggt gtgctagctg
      181 gcggtactgg gctggctttg ggctatttcc ttcgagagaa ataaacactt ggggcggttt
      241 cgccgcccca agcttgtatt atcggtatta tctcgcctgt ccctgcttgg cgactgcttc
      301 tgctgccaag cggatcgcct ctgcgtctcc gaggtagtag ctcttgaggg gtttcagatc
      361 gtcatccagc tcataaatta aggggatgcc agtggggata ttcaattcga caatctcttt
      421 ttcggagata tcgtccaggt atttgaccaa agcgcgcagg ctattgccgt gggcggatat
      481 cacgacgcgc tggcctgatc ttaccgccgg agcgatggtc tcgtgccagt atggcagcat
      541 gcgcgccacg gtgtctttca atgattcgca ggcgggcaaa tcatctttgg tgagatcagc
      601 gtagcgggca tcaaagcgcg ggtggcgtga atcatcccaa tctagggcag gaggtggagt
      661 atcatagctt ctgcgccaaa tcttgacctg ctcctctcca tattcgacag ccatctcagc
      721 cttattcaag ccttgcaggg cgccgtagtg tcgctcgttc aggcgccagt tacggtaaac
      781 cggaatccat tccaggttca tgtcttcgag cactatccaa agcgttttga tagcccgttt
      841 aagcacagat gtatatgcca gatcgaaggt gaagccttct tctgccaaca acttggcggc
      901 tgtatgggct tcagtctcgc cctgaggcgt cagaccgaca tctgtccagc ctgtgaagcg
      961 attttccaga ttccaggtgc tctgtccgtg tcgaattaag acgagtttgt gcatggttca
     1021 aaaaaatcct ttcatcgttc agcctcgagt aagccgtaat ccgcatctga aattcagcct
     1081 gatgagagct tggtactcga gaagtgcggg caaattgcgc ccgcactcaa aaatgttcct
     1141 ataaccttcc caatgacaag agaagggttt aatagatagt cacccgatac gtaagttagc
     1201 ggatggcttg ctctgaatcc ttatcaaaga tgtggatatt ctccatattg aaaaatacct
     1261 gcacatcatc attgatggag tagcgcgagc gggggtccac acgggcaaca aagttgttgt
     1321 tgccagactt taagtaaaca aatatctcat tacccatcaa ctcggttaca tccaccttgg
     1381 catttaccgg ttgtgtctga attccggggg gtgcgaaatt aggattgtga atatcttcgg
     1441 gccggatacc gaagatgatc gatttgcctt catgaggctt gtatacatcc atgcgttcgg
     1501 tggggatctg tacctggaag gctgtggcat caacaatgag attcccgtcg gatttgacaa
     1561 gctttgctgg gaagaaattc attgccggcg agccgataaa gccagccaca aacaggtttg
     1621 caggggtatc gtacagggtc tgaggggtgt cgatttgctg gagtaggcct ttattaataa
     1681 ccgcgatgcg cgtagccata gtcatggctt cggtttggtc gtgggtcacg taaataaaag
     1741 tggtctgcaa ttggatgtgc agcttgctga tattggcacg cgtctccaca cgcagcttgg
     1801 catccaggtt ggagagtggt tcatcgaaga ggaaaacctt tggctcgcga acgatggcac
     1861 gtcccaccgc gacccgctgg cgttgcccac ccgataactg gcgaggtttg cgatttagca
     1921 aatctccgat gcccaagata tcagctgcct ccttaacccg gcggtcgatc tcagctttgg
     1981 acattttgcg caatttgagt ccaaaagcca tgttatcgta taccgacatg tgcgggtaga
     2041 gggcgtacga ttggaagacc atagcgatat cgcggtcctt aggagcgacc tcattcacta
     2101 cctggtctcc gattaatatt tgaccttcac taatgtcttc cagtcctgcc agcagacgga
     2161 gagctgttga cttgccacaa ccggaagggc caaccagaac aagaaattcc ttgtctgcga
     2221 tctggatgtc caaatcgttg acagctgtga catccccaaa tcgtttgtag acatgctcat
     2281 atgttacact tgccattggt ttttctcctt tccacacgag tatagtgaga ttattatagc
     2341 cagacttcaa tgaaaatgca atgaagtgtc cactgtagtt cactgttttt ttatcaggct
     2401 tgctcttttg cttcacacaa cttaaaatca agacatgtct ccgaaagatg atatcagccc
     2461 gatccggaag caatacctgg agattaagcg ccagtatcca aacgctatcc tgtttttccg
     2521 gctgggtgat ttttacgaaa ccttcgacca ggatgcagag gtgacctcgc gcgagctgga
     2581 tatcgtgctt acctcgcgca acgttgctaa aggtactcgc gtgcccatgg caggcatccc
     2641 ccatcacgcc gcagaaaact acctggcacg cctgattgag cgcggctacc atgtggcgat
     2701 atgtgagcaa gttggagatc aacccatcaa gggcttgttc cctcgccagg ttatgcgcgt
     2761 ggtaacaccg ggcacggtca tcgagccagg cttgcttcca ggagatgcga acaattatct
     2821 ggcttgtgtt gtgatttcag aggatgaaga cgtaaggcgc gctggagtag cctatgcaga
     2881 tatcacaaca ggtgagtttg ccaccactga gcttcaagag ggtgacttac aaacgtctgt
     2941 gcgtgctgag ctaattcgtc ttatgccagc tgagattctg catccagata gctataagac
     3001 ggggaacggt ttgccagggc atctcactgc ttggcctgcc tggcgattcg aattgggtcg
     3061 ctgccaagaa gctttactca agcattttga gaccgctaca ttggatggat ttggcttgcg
     3121 cggcttgcca cttgccgtac gcgctgccgg ggcaatctta caatatctcg gtgaaacaca
     3181 agctgcttct ctcaagcaat tatcccgcct tagtgtctac agcctcttgg atttcatgac
     3241 tctggatgct gccacgcgcc gtaacttgga gctaactgag acgattcggg gtagctcaag
     3301 caaaggctcc ctgctgggtg tgctggatca cacgatcacc ccgatgggca aaagattgat
     3361 ccgccagtgg gtgagcaaac ccttgttgga tgttgaaaag atttgccaaa gacaagacca
     3421 ggtgcaattc ttttataccg agagcatgac ccgcaccgaa ttgagatctg ctctcaagcc
     3481 tttgggtgat ctcgagcgct taaccaatcg tgtggtcagc gggcatgccc aaccgcgaga
     3541 tttggtggcg atgaggaata cattgcgcca gttacccgga ttgcgtggaa tgttcccatc
     3601 gaccgatagc caaatggatc ctgttctaag ggatttgcac acatgttcag acgagttggg
     3661 gctattggaa gccgccatcg aagatgactc gcctgctaca ctacagaata ttggtgtgat
     3721 caggtctggc tattctgctg agttggacgg tgtagtggag cgctctcgac acgctcgcca
     3781 gtggatagcg ggattggagg gggtggagcg gcaacgcacg ggaatcaagt cgcttaaggt
     3841 gggctataac aaggtctttg gttattacat tgaagtgacc aaagccaatg tcgatatggt
     3901 accggcggaa tatatccgca aacagacgct ggtcaacgct gaacgctata ttaccccaga
     3961 gctcaaggag tacgaaaccc tggtgctgaa tgccgaggag cgcattcgcg agattgaggg
     4021 tcgtctattt cacgaggtat gcgcccaatt ggggggctca gcgactaagc tgttagccgt
     4081 tgcaggcgct ttggcgcagc tcgatgtgct ggcggcgctg gcagaagcgg ctatcctggg
     4141 aaattacgtg cgaccagatg ttgtggttga cgatgtgctg gaaatcaatg atgggcgcca
     4201 cccggtcgtt gagcaaatgc tggtaggaga gcgctttgtg cccaacgatg tgatgtttga
     4261 ggtttccgag cgggtgcgca tcatcactgg accaaatatg tctggcaaat ccacgctgat
     4321 ccgccaggtt gcgctcatag tgctcatggc gcaaatgggt agcttcatcc ccgctgacaa
     4381 ggcgcgtatc ggtttagtgg atcgtatctt cacccgaatc ggcgcacagg atgagatcca
     4441 tgccgggcag tctacattta tggttgagat ggtcgagacg gccaatatcc tgcaccatgc
     4501 caccccgcgc agcctgctga ttttggacga gatcgggcgt ggtaccagca cttacgacgg
     4561 ggtttccatc gcttgggcag tggttgagta tattcacaat cacccaaacc tgcgcgccaa
     4621 gacactcttt gccacccact atcacgagtt aacccaacta gcagatttgc tgcccggggt
     4681 tcgcaactat aacgtagctg tgagtgaggc agagggcaag gtagtctttc tgcacaaaat
     4741 catccctgga ggtgcggacc gctcttacgg aatccacgtt ggccaattag caggcttacc
     4801 caagccggtc atccaacgtg ccaacgagat catgcgttcc ttagaggctt cgtcgggcaa
     4861 ggcggtgcgg atcaacccca tggccgccaa gcaaatggct ctcttccctg aaaccaatcc
     4921 gctgttggat gagctcgaag atctggatgt caatgggctc tctccgattg aagctttgaa
     4981 taagctttat gagtggcaac agaagtatct gaataaatga cttcatgaga gaatcacgga
     5041 agcctaaccg cccgctctcc cctcaatcta acgatcgcac gatgttactg atggcgcggg
     5101 tagacgagat gcgagttgaa ttgcgcgcac aagatccttt caagttgatg caacgtgttt
     5161 ctgggaggtt tgtgcaaacc caatccggta aaggggaatt gcgcttggct ttgtgggggc
     5221 gcgaaatttt tgtcacattt cccgacttta tcgccttcga aggcagttca cagcagaaat
     5281 tgcacgcggg attacaggca ctggtaatct attacttgta cactacagat ggcgcaccca
     5341 tcgctgaccg atttatctcg ttttccgagc tacctgatgg caaattttat gcccaggctt
     5401 ttcagggata caccggtcaa gaactgcaac atcattttgg ggatgatcgc caacgttttg
     5461 aacgagctgc tcagaatatc ggaggtaggc attatccact tggggacgct tctttcacct
     5521 ttcaacttct tccccgtgtg gttctattgg ttgttttctg gtgtggcgat gaggatttcc
     5581 catcctccta tcaggtgtta ttcgatgctt ctatttccca tcacctgcca acagatgttt
     5641 gcgccgttgc cggaagcatg ctcacccgac aattgattga atctgaataa tatacaagta
     5701 aaatttttat gcggaactat ctacgattga tactgttcgt cctactattc gccggggtgc
     5761 taaatacaag ctgtgcagga aaccagggga ttagcaatcc tgaaactgcg acagcacagg
     5821 ctggtcgtat tattggtttg gcaacccaaa tgggaggata cgcccaggca acccagatgg
     5881 ctgaaaatct ccaagtgacg gcgaccgctg ctgccaggca gctcttgcta gatgaagtgg
     5941 ggcagtggcg cctcctgctt tcagactcat atgatgaaga cctaaacgat tggagcacag
     6001 ggagtgatga agatccggag ctgggttcga taagctgggt gattgaagac ggaaaatatc
     6061 gctggcaagg gctcgcaacc aacggttttg tgtggtgggt cattccagat attgaaagcg
     6121 tttcggattt ctatctatcg gtctctgccc ggcaggaggc ccagcctgaa gtgggtgaat
     6181 atggtctggt gttccgccaa tccggagatg tagactatta cctgtttgag atcagtgatt
     6241 taggcctgta ttccctgtat ctgcatactg acagtggttg ggaatcattg atcgattgga
     6301 cagctcatcc ttctatcaat cctggtgcaa caaaccgtct agcggtgatt gccacaggcg
     6361 agcattttga tctctttata aacgatactt ttgtggctga atatagcgac gaccggttac
     6421 ctgaggggaa ggcgggcttg ctagtaggcc tctccaatcc cggggatgct gcgaactggg
     6481 aattcgatga ttttgaattt cgagccccat gaggggaggt cattgattaa ccttttcatc
     6541 aggtattctg tatgtataat atcgatataa gttacgcggt tggaggaaaa taaaccccaa
     6601 cggtgggact tttgtatcga tttataattc caatatgatg ccctaaggga gaccagtaat
     6661 gaaaaagttt cgcgtggctc tgctggcaaa tttgaaggtc aatgctccaa agtttgaagg
     6721 tatgccggaa gaccagtggg atgatttgga ttcagaaaaa acgatcaatg cgctggtcaa
     6781 cgccattcgg gcaggaggaa attcctgcga gttcctggaa gggaatctct cgctggtaga
     6841 ttcattgcag aaatttaaac ccgatatctg ctttaatatc tgtgaaggac atttcgggga
     6901 cgcacgcgag gcgcatgtgc cagcgttact tgagatgctg cgcataccat acactggctc
     6961 cagagtgctt acactcgctc tggcgctgga taaacccatg accaagcgtg tcctggctta
     7021 tcacgacctg cctacgccgc catttcaaac ctttgagcgg gtggatgagc ctttaagcac
     7081 cgatatgaac taccctctgt tcgttaagcc cagtcgagaa ggcacgggta tgggtgtttc
     7141 aggcaggtct atcgtgcgtg atgaggatga actgcgcgag caggtggact tcatccttaa
     7201 acgctacaaa caacctgcac tggttgaggg ctatattgaa gggcgcgagg tgaccctcgg
     7261 agtggtgggt aacttgattg ggcctgtcgc caggcgcttg ccggaagatg atgaagcccc
     7321 gcgcattcaa tcgggcttgc gctttttccc acccatggaa gtcaacctga agccgtttga
     7381 agacagcgat gtggtatata gtggcaggct caaagtggac atggcggatc aattggatta
     7441 tctctgcccg gctccactgg actcggacat ggtcgatgaa ttgaactggc tcacggcagc
     7501 ggttttccga gtgaccggcg ccatggatgt ctcgcgcgtc gatttccgcc tggatgtcca
     7561 cgacaattgg aagccttata ttttggagat caatccgctg ccaggtctgt ctccagggat
     7621 atcagatctg gtcatcgaag ccgctgctga gggtgtagac catacttacc tggtcaatat
     7681 gatcttagat acagcgctcg aacgctatgg gatgatttaa tcatcccatg acccccatgc
     7741 ataacgcaaa gttacgccca taatatacca tcctgctaag atggttcccc gcaacgtact
     7801 gctgattttt gaatgtccgg cctggcgggg atgaaagctg accggcactt ctttaattcg
     7861 tgcccctcgc cgttgggctt ttacggtcat ctcggtgggc catccgtaag tcatctctcg
     7921 catattgagt gttgagagca gcgactttcg tatcgcccga aagggaccta aatccgtaat
     7981 ccttgatcca taaaggaagt tcatcaagcg agatgctatc caattcccaa aacgttgttg
     8041 gaagggcatc gcaccagcgg cgatatgccc aagaacgcgg gagccctgca ccaggtcagc
     8101 ctccccggat tgaattgggt ttaccaaggc agggatctcc gatggcagag agctgaaatc
     8161 cccatccaga aagaccagga tctcgctacc ttctgacgca gctaccccag cagcacaggc
     8221 ataaccatat ccacgccttg gttcttggat cagttgtgcg cccgcgcgga tggcttcatc
     8281 agcagtttcg tctgtggaac cgttgtccac tacgatgatg tctgcattta cagtggagcg
     8341 cacttcgccg atcaagcgaa agaggttgcc agcctcattc aaagccggga taataacggc
     8401 gatttcgctc atcgagttga taacatgttg atttgtttga ggtaccggcg agtctgcggg
     8461 gctattagtc ggggtgattg ctccaaatca accagcagtc gatccaaggt ctcgacatca
     8521 tctatgtcgt accagccggg taacagatga acacgcagcc ccaagctgtc ggcaattctt
     8581 aaagtgtctg aagtgacacg gggcgtgctc atatgcacat cgcgcagcaa gcggggtgcg
     8641 ggcttcttca gtccaattaa gtaataacca ccgtcttcgc ttggacccag gactacgtca
     8701 acttgtgcga gggctttgtg ggctttgatc aagtactcca ccggtaaggt ggggccatcg
     8761 ctgttcatga taacgacttg tgtgaagcca ttaaggagat agtaagaaag cgcattgtct
     8821 agccggctgc ccagattaga tccggtttgt ggtatgagct caaagtcggc tgctagcttg
     8881 ctgaagtagc ctgaagcact ttttggcgta aacgcaataa ctttgtgcac atcaggtacc
     8941 tggcgaacaa ggtccagcgt atccagtaga aaacattcat acagcccgcc agccgctttg
     9001 ggagagagcg gtgggctgag gcgtgtttta gttctcccag gtgaggggcg cttggccata
     9061 accaataagg catgcagggg gagcgacata cacaattatc ttaaaacata gccgaaacag
     9121 tgttttgtgc cattgaggtg atcatactaa cccgagtcga atcaaatcgc tatgacttat
     9181 ccggtagaac gcggccgttg gtcttttgcc attcttcatc ttccactagg gttactaccc
     9241 gaaaagtctc cgcgtagacc atttccttcc ctttagcgcg ctccgcagcc cattctttaa
     9301 tgcgtggttc gggatcccaa tcttgcattt ggctcttgtg ggcgcgcagc gctgcaattt
     9361 tgatatcaat agtttctgtg atgttgacat acaactcatg ctgcacccat ccggtcacat
     9421 atacttttcg cggtttgtga gcgtgtatgc cttcttcttc gagttcttca aacaggttgg
     9481 gttgcccagc ggcagggaat atcgcatcca gagctgcgga ggcagcggct cggtggtctg
     9541 ggtggttgat gtagtcgtta ccgacccaaa cgatggtcgg atcaccacac acgacaacct
     9601 cgggtcggaa gcggcgaatt tctcgaacca acttcttccg taattcaatc gtgggaacca
     9661 acataccatc cggctcgcgc aaaaagacca catcctttgt cccaacgatc tccgcagctt
     9721 ggagctgttc tgcctcacgg atggcaatag cttgtgcttt tgtcatacct ggctccgcga
     9781 tccctacatc accgcttgtg cagagtacgt agcacaggcg agttcccgcc tgagcccagc
     9841 gcgccaatgt cccagcacag gagaattcga tatcatctgg gtgcgctacg atgatcaaag
     9901 cactctcggg gatataaaaa ctgttattca tatttggact cctccagtaa gatcacagcc
     9961 cctattctaa cgcttgggcc tgattcaagc aataaatgtg tattgcaata aaatggtgta
    10021 tgggttaaac tctaaagtgg agtggtttac tccactattc tctgttgtgt catcattttt
    10081 atattcacga atattaccga gtatattagt accaatagaa ggaggtggaa atggatagat
    10141 acttgatcga gacccctcac accgatgagg attgccaaag gctgatcgat aacgtgtatg
    10201 caatggggta tatttaccac ttcgattggg gttgcgacgc tggtgtgcat tgcggctggg
    10261 cgattatcga agcagaagat gaagaacagg ctcggctcgc agtcccttca ctggtgcgaa
    10321 acaaagcgcg cgtcatcaag ttgaaaaaat atacccccga gactcttgac gaccactagg
    10381 atgcaagata acagaaagtg caaagagcca tgtattacac gcatttccac gttatcaacg
    10441 cgtgccttat ggtccgggat gggtgttggt tgga
//
LOCUS       DBNGSX010000477         6274 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1502405, whole genome shotgun sequence.
ACCESSION   DBNGSX010000477 DBNGSX010000000
VERSION     DBNGSX010000477.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6274)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6274)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6274
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1502405"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            356..1645
                     /locus_tag="AC16CB_14110"
     CDS             356..1645
                     /locus_tag="AC16CB_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3343651.1"
                     /translation="MTKRLFTLMSLLVLTTMILAACAPQAAAPEAPEAPAPAEFECTD
                     AIGCVEIAPDEPVHIAFMLTISGATAFLGEDSKGAVEIAVDDRGGKLLDHDIQLTGED
                     SGCSAEGGQTAATKVAADPTILGVIGTNCSSAATAAMPTISGAGLVMLSPSNTAPALT
                     IEGETWQPGYFRICHTDLFQGSVAADYAYNELGATTAATIHDGSPYADQLQQVFSDRF
                     QELGGTITFQGAVNVGDTDMRTILTSVAADSPDVLYYPIFEPEGPFITAQSSEIAGLE
                     NTTLMGADGLLSDSFPEAAGANSVGMYLSGPYVSGAPYDEFLAKWEAKFGGTPPSGFH
                     AFAYDATNILLDAIEKVAVVQDDGTVFVGRQALRDAVKATDNYDGLTGKLSCADKEFP
                     GVGTSKGDCATGDALGIFELTQAEVDGNWPPPVVYTP"
     gene            1776..2840
                     /locus_tag="AC16CB_14115"
     CDS             1776..2840
                     /locus_tag="AC16CB_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3343652.1"
                     /translation="MLKRYLERFSLVSLFLNLFRIVAIIILIIGVYNTVTAGRYTSAQ
                     WATLLFAGLAQGSIYALIALGYTLVYGILLMINFAHGEVYMAGAFTAVFLAQYHAASG
                     FLDRQPLLAILSWIALALVVSTVIAVLLERIAYRPLRNAPRLVPLITAIGASFALQYT
                     FRGMYGPDFRAYPEVQMLRGLVNVFGFQVPLVQLVVLFAAILLMIGLYLFVERTKQGK
                     AMRAVSEDKDVARLMGINIDRVIVTTFALGGGLAGAAGVFNGLFRPQGIYFFMGFFPG
                     LKAFTAAVLGGIGNVPGAMIGGLLLGVIESIFPNLILEGLGIPAANQLRDAFAFTVLV
                     LILIFRPQGIFGERLAEKKA"
     gene            2850..4577
                     /locus_tag="AC16CB_14120"
     CDS             2850..4577
                     /locus_tag="AC16CB_14120"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3343653.1"
                     /translation="MSNNSTQPSWRIFLNYGLIGSVITLYLAVIGMVEAFATRNLIGT
                     YLSMGQIFLITGAVVSGILAARAFRDKSTLSAVGASLAAGMISSSTLLLLILFIQIFV
                     IPQTGGNVLFNWRNMFVNFSPGLVEILTFGLGPALGSLALVIISGGLGMIGSVFMLLP
                     SRWRNALISGVAWTLGIGIFSENVAQIVQEIIGRGLVRILFQNKSLNPTAAVVIFVVA
                     FLVGYFRVHRQAGNQWGKLSPAQQKQGRFIGIVLLIAFILALPWMIGLFLSQALFIVG
                     YFIMMGLGLNIVVGYAGLLDLGYVAFFAFGAYTMGVLTSQGPLGVSNANFWVALPISV
                     LVGVIWGLLLGFPVLGMRGDYLAIVTLGFGEIIRILALSDWLAPLQGGAQGVLHIPPP
                     NLFGVQMNTPQSMYYLVVFGSLLAAFVTIRLRESRLGRQWMAMREDEDVAEAMGINLV
                     ETKLLAFAIGAAFSAAAGAIFAARLSNIFPHSFNLIVSINALALIIVGGLGSIPGVIV
                     GGLILVGLPELLREFTEYRLLMYGILLIVMMLVRPEGFLPEATRKRELHAGEENYIEP
                     QAAATEMEG"
     gene            4583..5389
                     /locus_tag="AC16CB_14125"
     CDS             4583..5389
                     /locus_tag="AC16CB_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3343654.1"
                     /translation="MGTILSTSNVTKRFGGLVAVDKFDVNIQENSISSVIGPNGAGKT
                     TFFNCITGFYAPDGGEINFNGHIIQGLSTDRITRFGISRTYQNIRLFANMTAIENILV
                     GMHPKLKTLWIESLFKSRRFKQEEAEALEEARRLLRFVGLAGLGDQLARNMPYGAQRR
                     LEIARALANKPALLLLDEPTAGMNPQETVEMTHFIRNLRDELGTTILLIEHDMRVVMG
                     ISEQISVMDFGQKIAEGKPADIQRNPRVIEAYLGRGAASGLGAASAQPAN"
     gene            5421..6146
                     /locus_tag="AC16CB_14130"
     CDS             5421..6146
                     /locus_tag="AC16CB_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3343655.1"
                     /translation="MPMLEVNDIHTYYGNIHALKGISLTVEKGEIVTLIGANGAGKTT
                     TLRTISGLLKPRQGSVSLEGEDISSPTSPYSAHDLVYKGIAMVPEGRGVFAKLSVLEN
                     LEMGAYSRKDKNGIQKDFDLVYTLFPRLKERRTQVAGTLSGGEQQMLATGRALMAHPS
                     LLLMDEPSMGLAPVLVESIFDTIEQIKKEGTTILLVEQNATMALSVADRGYVLQTGSV
                     VVADTAANLQQNEMVQKAYLGIE"
BASE COUNT         1384 a         1731 c         1629 g         1530 t
ORIGIN      
        1 atacggatcc atccctcgga tatcgcttga tgcgcaactt ggctgcagac ctggcattaa
       61 aaatccgtaa cacagattta tcgatacgtg ggcagctact ttaccgtaaa aatttttcat
      121 aaatatcatg agctagtcct tgaccatatc ggatgttatc ggtaaaatcg ttgtcatgtg
      181 cgtccttcat aacaaggata gctttgcagt tcattacttg cctcacttca atctaaactg
      241 cattacatcc aagagtaacg agaatcatca tcgagaggag gtggtttcgg aaaacagtaa
      301 aaaaagtaat gcctcacaca acacaatgat cccaatatat taaggagaga agaagatgac
      361 aaagagattg tttaccctga tgtcgttgct ggtacttacg accatgatcc tggcagcatg
      421 tgccccacaa gctgctgccc ccgaggctcc agaggcgcct gctcccgcag agtttgagtg
      481 caccgacgcc atcggctgcg tggagattgc acccgatgag cccgtccaca ttgctttcat
      541 gctgaccatc tcaggcgcca ccgccttcct tggcgaagac tcaaaaggtg cggttgagat
      601 cgccgtggac gatcgtggtg gcaagctgct cgaccatgat atccagctca caggtgagga
      661 ttccggctgc tcggccgaag gtggacagac tgcagctacc aaagtcgctg ctgaccccac
      721 catcctgggt gtgatcggca ccaactgctc cagcgcggcc actgcagcca tgcccacgat
      781 cagcggtgcc ggactggtga tgctttcccc atccaacacg gctcctgccc tgactatcga
      841 aggtgaaacc tggcaaccag gctacttccg catctgccac accgacctgt tccagggttc
      901 tgttgctgca gattatgctt ataatgagct tggggcaacc actgctgcga ccatccatga
      961 cggcagcccg tacgctgacc agctccagca ggtcttctca gaccgtttcc aggaattggg
     1021 cggcacgatc accttccagg gcgcggtcaa cgtgggcgac accgatatgc gcaccatcct
     1081 gaccagtgta gccgccgata gcccggatgt gctctactac cccatcttcg agccagaagg
     1141 acccttcatc accgctcaat cttccgagat cgctggcctg gagaacacca ccctgatggg
     1201 tgccgacggc ttgctgtctg actcattccc cgaggcggct ggcgccaact cggttggtat
     1261 gtacctgtcc ggcccctacg tttcgggtgc accctacgac gagttcctgg ccaagtggga
     1321 agccaaattc ggcggcactc ccccgagcgg cttccacgcc tttgcctatg acgctacgaa
     1381 tatcctgctc gacgccatcg aaaaggtcgc tgtagtgcag gatgacggga cagtcttcgt
     1441 cgggcggcag gctctccgcg acgccgtcaa ggctactgat aattatgacg gcttaaccgg
     1501 aaaactgagc tgcgccgata aggaattccc tggcgttggc accagcaagg gcgactgcgc
     1561 cactggtgac gccctgggaa ttttcgagtt gactcaggca gaggtcgatg gcaactggcc
     1621 tccgcccgtt gtctacacac cataatgaga cataaaaagt tacacgtaca taatagccta
     1681 gtcctataat gcgaggcgag ccaaagggtt aacgctctct ggctcgccga tgcttaattt
     1741 ttgagcatcg ctaatcaaac gaaaagaggt tcagaatgct taaaagatac ctggaacggt
     1801 tttcacttgt cagcctcttt ctcaatctat tccggattgt tgccatcatc atcctaatta
     1861 tcggtgtcta caacaccgtg actgctggaa gatacaccag cgcccagtgg gctaccctgt
     1921 tgtttgcggg attggcccaa ggcagcatct atgcactcat cgctctgggg tatacgctgg
     1981 tatatggcat tttgctgatg atcaactttg cccatggcga agtgtatatg gcgggggctt
     2041 ttacagccgt attcctggcc caatatcatg ctgccagcgg atttctcgac cggcaacccc
     2101 tgcttgccat tctctcctgg atagctttag ctttggttgt ctccactgtt attgctgttc
     2161 ttttagagcg cattgcctat cgccccttgc gcaatgcccc acgcttggtg ccattgatca
     2221 ccgccatcgg agcttcattt gcgctccaat acacatttcg cggcatgtac ggtcctgatt
     2281 tccgggctta ccccgaggtg cagatgctgc gcggcctggt gaatgtattc ggatttcaag
     2341 ttcccctcgt ccaacttgtg gtcttattcg ctgccatact cttgatgata ggcttgtacc
     2401 tgttcgtcga acgcaccaaa caaggcaaag ccatgcgcgc tgtttcagag gacaaggatg
     2461 tcgcccgctt gatgggcatt aatatcgatc gcgtcattgt cacaaccttc gccctcggcg
     2521 gtggcctggc tggcgcagcc ggtgttttca atgggctgtt tcgtccccaa gggatttact
     2581 tctttatggg cttcttccct ggtctaaaag ccttcacagc cgccgtgctg gggggcatcg
     2641 gcaacgtgcc aggcgccatg atcgggggct tgcttttagg ggtgatcgaa tcgatcttcc
     2701 ccaacctgat cttggaggga ttgggcatac ctgccgcaaa ccaactgcgg gatgcattcg
     2761 cattcaccgt gctggtccta atcctcatct tccgcccaca aggtattttc ggcgaacgct
     2821 tggccgagaa gaaagcgtga gtatgcgata tgtcaaacaa tagtacacaa ccaagctggc
     2881 ggattttcct gaattacggt ctgatcggta gcgtgattac gctctacctg gccgtcatcg
     2941 gtatggtaga agccttcgcc acacgcaacc tgattggaac ttacctctcc atggggcaaa
     3001 tcttcttgat caccggcgcc gttgtctccg ggatattagc cgccagggca ttcagggata
     3061 aatcgaccct gagcgcggtg ggtgccagcc tggcagccgg gatgatctcc agctccactt
     3121 tgctgctgtt gattctattc attcagatat ttgtcatccc ccagaccggc ggaaatgtgc
     3181 tctttaattg gcggaatatg tttgtcaact tctcgccggg tctggtcgag attttgacat
     3241 ttggcctcgg acctgctctc ggtagcctgg cactggtaat cattagtggc gggctgggta
     3301 tgatcggctc agtattcatg ctcctgcctt caaggtggag aaatgctttg atcagcggtg
     3361 tcgcctggac cctgggaatc ggcatcttct ccgagaacgt cgcgcagatc gttcaagaaa
     3421 ttatcggcag gggtttggtg cgtatcctgt tccaaaataa atctctcaac cctacggcgg
     3481 cggttgtcat ttttgtagtc gcttttttgg tcggatactt ccgggtgcac aggcaagcag
     3541 gcaaccaatg gggaaaactt tcaccagccc aacagaaaca agggcgtttc atcggcatcg
     3601 ttttgctgat cgcttttatc ttagccctgc cctggatgat cggcctattt ttaagccagg
     3661 ctctcttcat cgtgggttac tttattatga tgggtctggg actgaacatc gtggttggtt
     3721 acgctggtct gcttgacctg ggttacgttg cctttttcgc cttcggggcg tacacgatgg
     3781 gcgtgctcac ttcccaaggt cctctggggg tcagcaacgc gaatttttgg gtcgccttgc
     3841 ccatcagcgt attggtggga gtgatttggg gcttgctgct cggctttccc gtgctgggca
     3901 tgcgcggcga ctatctggcg attgtaacgc ttggatttgg agaaattatt cgcatcctgg
     3961 ccctctctga ctggctagcc cccctgcaag gcggggccca gggggtgctc catatcccac
     4021 ccccgaatct tttcggtgta caaatgaaca ccccgcaatc gatgtactac ttggtcgttt
     4081 ttggctcgct cttggcggct tttgttacca tccgtttgcg cgaatcgcgc cttggacggc
     4141 aatggatggc tatgcgcgag gatgaggacg ttgccgaagc catgggcatc aacctggtcg
     4201 aaaccaagct gctggctttc gccatcggtg cagcattttc agccgcagcc ggagccatct
     4261 tcgccgctcg cctgagcaat atcttccctc acagcttcaa tctgatcgta tccattaacg
     4321 ccctggctct gatcatcgtg ggtggcttag gcagcatccc tggcgtcatc gttggcgggc
     4381 tgatccttgt cgggctgcct gaattattgc gtgagttcac ggaatatcgc ctcctgatgt
     4441 atggcatctt gctgatcgtg atgatgttag tgcgacccga aggcttcctt cccgaagcca
     4501 cccgcaagcg cgagttgcat gcaggagaag agaactatat cgagccgcaa gcggcagcca
     4561 ctgagatgga gggttaagcg atatgggcac aatcttatct acaagcaatg ttacaaaaag
     4621 gttcggtggt ctcgtcgctg tagacaaatt cgatgtcaac atccaagaaa actcaatttc
     4681 aagtgtcatc ggccctaatg gagccgggaa aacaacattc ttcaactgta tcaccggttt
     4741 ctacgcgccc gatggaggcg agatcaactt taatggccat atcattcagg gactttcaac
     4801 cgaccgcatc actcgctttg gcatttcccg cacgtatcaa aatatccgcc tgttcgcaaa
     4861 catgaccgcc atcgagaata tcttggtggg aatgcaccca aagttaaaaa ctttatggat
     4921 agagtccttg tttaagagcc ggcgtttcaa acaggaagaa gccgaggcgc ttgaagaagc
     4981 tcgtcgcctg ttgcgctttg tcggattggc tggtctaggc gatcaactgg cccgcaacat
     5041 gccatatggc gcccaacgcc gcctggaaat tgcccgtgcc ctggcaaata agcccgcgct
     5101 actcttgcta gacgaaccaa ctgccggcat gaacccgcaa gagacggttg agatgaccca
     5161 tttcatccga aatttgcggg atgagttggg taccaccatc ttattaatcg aacacgatat
     5221 gcgcgtagtg atgggcatca gcgagcagat ttccgtgatg gatttcggcc aaaaaatcgc
     5281 tgaaggcaag ccagccgata tccagcgcaa ccctcgcgtg atcgaagctt acttgggtcg
     5341 cggtgctgca tcaggattgg gtgcagcttc agcccaaccg gcaaactgaa tccaccggtg
     5401 agattttgga gcaaatcacc atgccaatgc ttgaagtcaa tgatatccat acgtattacg
     5461 gcaacattca tgccctcaaa ggcatctccc taactgttga gaaaggcgag atcgtcaccc
     5521 tgatcggcgc caacggtgcg ggtaagacca ccacgctacg cacaatcagc ggcttgctca
     5581 aaccgcgcca gggcagcgtg tcgctcgaag gcgaagatat ctcatccccg acctcaccat
     5641 actcggccca tgatctagtc tacaagggca tcgccatggt acccgaaggg cgtggtgtgt
     5701 ttgccaagtt gagcgtcttg gaaaacctcg agatgggagc ttattcccgc aaagataaaa
     5761 atggcattca aaaagatttt gacctggttt acacactttt tccccgtctg aaagagcgcc
     5821 gcacgcaagt ggctggcacc ctctcgggtg gtgagcagca gatgctggct accgggcgcg
     5881 ccctgatggc tcacccgagc ttgctactga tggacgaacc gtccatgggt ctggcgccag
     5941 tcctggtcga gagcatcttc gatacaattg agcagatcaa aaaagaaggc acaaccatcc
     6001 tactggtcga gcaaaatgcg accatggcgc tttcggtggc tgaccgtggc tatgtgctgc
     6061 aaaccggaag cgttgttgtt gccgacaccg ctgccaacct ccaacaaaac gagatggtgc
     6121 aaaaggccta tctaggaatt gagtaataca aggacatccg cgcaacgaca aactcatccc
     6181 ttgtggctcc acatgatcta tctcgaagat acattcgagg tacgcaaatc tcgagcgcag
     6241 tggtggtaaa atcctgccac tgcgcttttc ttat
//
LOCUS       DBNGSX010000478         9426 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1502467, whole genome shotgun sequence.
ACCESSION   DBNGSX010000478 DBNGSX010000000
VERSION     DBNGSX010000478.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9426)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9426)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9426
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1502467"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="AC16CB_14135"
     CDS             complement(<1..282)
                     /locus_tag="AC16CB_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343656.1"
                     /translation="MDIARRLGIFQPVLFGSTYERKVKNLFGSALLAYWPLWENSGSA
                     AHDISGKGRDGSYSGVALGEQGIGDGHTCPFFDGASAYCNVYSTDLRDVF"
     gene            complement(261..545)
                     /locus_tag="AC16CB_14140"
     CDS             complement(261..545)
                     /locus_tag="AC16CB_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343657.1"
                     /translation="MPATFSGQKTVTAAGTAERLHTGLQVNCPVMVKALPANTGTMYV
                     GGVAGDVSSSNGMPLAAGEVAVFEFIGDLSEIWVDSTVNGEGVAWISLDV"
     gene            complement(549..839)
                     /locus_tag="AC16CB_14145"
     CDS             complement(549..839)
                     /locus_tag="AC16CB_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343658.1"
                     /translation="MIPAPGGALRLGRDGYGVTLMVAKSGKSRLELAASILGCEAALI
                     LGYRETPQGGIVVIAPDGRKLKFTAEDLYGQRAAKPNTGRGTGAQKTGKKGK"
     gene            complement(841..1350)
                     /locus_tag="AC16CB_14150"
     CDS             complement(841..1350)
                     /locus_tag="AC16CB_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343659.1"
                     /translation="MGYIHDTAMSKFLGPEEAQFSAGTWTDSVATNVWSKNRSAADAA
                     FVIKIPIKLNQNSASYKGSKLASINIWYEVATAAMDSVAATIYKAALPADSAAFAAPV
                     SQSFTYDTGHDTAAERIDVDEHKMTLNLDTPIWLDDDDEVYAELSCDAAATSVFKYFG
                     ARANFTLRI"
     gene            complement(1475..3505)
                     /locus_tag="AC16CB_14155"
     CDS             complement(1475..3505)
                     /locus_tag="AC16CB_14155"
                     /inference="COORDINATES: protein motif:HMM:NF051749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HIR3343660.1"
                     /translation="MSDEQQETHLILAAEGWPAQSARPGQHTKAGFEVLAITAGNGNG
                     WEFSEEVLRASLSLWDGTSCFVDHAFWSGHSVLDLAGVFSNPRWNEERRGILVELVTS
                     GPSGPLVDQLGREMLREGKPKPRVGFSADLWFTCKGRVVERITRVESLDLVINPARGG
                     AFIRALNATRGTNSQPPSGGIQKGAKIAMDETTPTPDGRQAATPAGESLAQNELEAAR
                     SIPASQKEWQAAQQLRLQMCQYLLDSGLAAAKLPATVQEHLRKQFAGKLFEPDELQQS
                     IDSARELVSQLAGSSAVIGPGRVHGVFSSEDQLQAAVDDLMGAPRDKGMEGLKVARLS
                     GIRELYLMLTGDEELHGGYYPSRVRLATTADFAGLVKNALNKIVVNQWGELGRAGYDW
                     WQRISSVEQFSSLQSISGTLIGTVGTLPTVAEGAEYTELTVGDSPETANFTKYGGYIP
                     LTLELIDRDETRKLRAYPRELANAGLRKVSALVAALFSDNSGTGPTMADGEALFNATA
                     VTTAGGHANLRTTALSTAEWEAVSQAVYNQPMLIKNAAGVYGTGPKQAINPRYLLVPR
                     ALELTAKKILYPFWENAANIHSENQQRGEMGDVVVVPEWTDANDWAAACDPRIAPAIF
                     VGTRFGLMPEVFLAGSELSPAVFMNDEHRLKVRHFIAVWVNDFRPLHKSNVA"
     gene            complement(3518..4867)
                     /locus_tag="AC16CB_14160"
     CDS             complement(3518..4867)
                     /locus_tag="AC16CB_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343661.1"
                     /translation="MLSRFVGFFFGKEIQRQASLAVRVLDDKTDRLAASWSTYVSTYP
                     RDRHGYDRDEILRDALDAWRQNPLARRVVELMSQYVVGGGLGVECRHERTHRFLQNWW
                     NHRLNRMPLRAFDLCDELTRTGNLFIVVSTDAAGMSYIRTLPTQDILDIETALNDIEQ
                     ELVVWERPRGDGTEQAAPNGFMGHAWPVYDERTDRQDADGSFPPAVLHYAVNRPVGAK
                     WGESDLAPLLRWLSRYSAWLEDRARLNRYRQSFLYVVKAAFTSQAERLARQAELNANP
                     PNPGAILVADESETWSVLNPELASFEAAEDGLAIKKMIAVGSGNPLHFLAEPESATRT
                     TAEAAGGPTFRHYEQRQKFFLWMIEDLARVVVQRRALVDRHVAPRAYISAKGTDISAR
                     DNTSLAAAANTIVSAFALLRKRGLIDDAELLRMAYRFAGEVVDVEEMLRRGAAAGSI"
     gene            complement(4867..6468)
                     /locus_tag="AC16CB_14165"
     CDS             complement(4867..6468)
                     /locus_tag="AC16CB_14165"
                     /inference="COORDINATES: protein motif:HMM:NF036421.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HIR3343662.1"
                     /translation="MNELLTYLPTLKSLVRDVRLFARHAAKLSLRSYQQSVALAIVES
                     VIEGRGDTIVVMFPRQSGKNELQAQVESYLLMLFFMANAEIVKISPTWKPQSHNAMRR
                     LERVLSKNFFTKFNWKKESGYIYRVGTARIFFLSGSQEANIVGATASTLLEVDEAQDV
                     LVAKFDKDIAPMAASTNATRVFWGTAWNSRTLLARELRAAREAERRDALAGRPVRQRV
                     FVITADEVAAEVPAYGKFVAEQIAKLGRDHPMVRTQYYSEEIDAEGGMFPARRLALMQ
                     GSHPRQTAPSAQPGRIYALLIDVAGEDEAMRADEGADAHVGLHAGSLANPRRDSTALT
                     VVEVDLSSLKDPVLRAPTYRVVDRRSWIGVRHSTLYAQIRALVEHWVARHIVIDASGV
                     GAGLASFLDRAFPNRVIPFVFNAATKSNLGWDFLTIVETGRFKDYATEESQRSAFNLQ
                     LSYCQMETLPGPDRRMKWGVPEGTSGGQDLDSLPGQAGKLIHDDWVLSAALCAVLDSL
                     EWRISAPTAVIQAVDPLKQMDREGF"
     gene            complement(6465..6842)
                     /locus_tag="AC16CB_14170"
     CDS             complement(6465..6842)
                     /locus_tag="AC16CB_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343663.1"
                     /translation="MEMNLNADETKPEERPRRKRGAQPGNLNALKHGLYSRRLQERGA
                     EADLEEEIITLRNLVQRTSQLATDPDMNLTTLLKVLDTIGSAMNRLANLLQAQKQLHK
                     SSTDANAVLLQAVIEVARELGIE"
     gene            complement(6862..7269)
                     /locus_tag="AC16CB_14175"
     CDS             complement(6862..7269)
                     /locus_tag="AC16CB_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343664.1"
                     /translation="MDAKRALTSPENLAPFSACTLDIPPNASGVSVPLETPESGGAKW
                     QKGACMDLDRFVLNPTTLLVLIFGLVEYIKGFGMRGNSLRAVSMALGVALAVAYRLRE
                     AAPDWAGWIEMAFFGLAAGLAASGVYDFLKNRL"
     gene            complement(7402..8715)
                     /locus_tag="AC16CB_14180"
     CDS             complement(7402..8715)
                     /locus_tag="AC16CB_14180"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR3343665.1"
                     /translation="MEASRPIPTPDSQTGLRALQAMLRERHPLAALQVFHDELGDVFR
                     IQLPGFTPVVLVGPQAARFVLVEERGELLWRNEADPVVALLRHGLLVEDGQAHDDLRR
                     LMNPALHRRMLERYLAEMSQAVDQVTSHWHDGDVVDMLVEMRKIALLILVGTLFGADF
                     QPELQPLWQPILDSIRFISPGIWMVWHDAPRPQYRSGLQRLDDYLYRLIRERRALGDF
                     RDAPDLLGSLIATGMTDDLIRDQLLTMLIAGHDTSTALLSWALFLLGSHPQAYQRAQD
                     EVDAFNFSGQVTIEQLSGLRYLDSVIREALRLYPPIHLGSRLAATDLDYQGYHIPAGE
                     RVIYSIYLTQRHRGHWQDPHRFLPERHASGGQKTPYTWLAFGGGPRNCIGAAFGQVEA
                     KLVLAIILHRFDLQLVERHVRPHMGATLEPHPGVRMRLKLRTPSQ"
     gene            complement(8718..>9426)
                     /locus_tag="AC16CB_14185"
     CDS             complement(8718..>9426)
                     /locus_tag="AC16CB_14185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3343666.1"
                     /translation="GVRLADGTYLPAKAVIANLDVTTVYEKMLPPEPAIRKRMMSLQK
                     TPSSCSGFILLLGVRGEHPQLKHHNIFFSSDYRREFEQIFKQGIPPEEPTIYVSITQR
                     STPEDAPPGCENWFVLVNAPPLGPAWDWRREAERYAGGLLDQLARRGFDVRNKLEYQG
                     IITPEDLEGMSGARGGALYGTSSNSRWAAFRRPHNRAPQVKGLYFAGGTVHPGGGVPM
                     VILSGKVASELLLEDGF"
BASE COUNT         1997 a         2859 c         2650 g         1920 t
ORIGIN      
        1 gaacacatcc cgcaggtcgg tggaatagac attgcagtaa gcgcttgccc catcgaagaa
       61 ggggcaagta tgtccatcgc caatgccttg ctcgcccagg gcaaccccgc tgtaagagcc
      121 atcccggcct ttgccagata tatcgtgagc cgcgctgccg gagttttccc acaacggcca
      181 gtaagccagg agcgccgagc caaacagatt cttgactttg cgctcgtaag tggatccaaa
      241 caggaccggc tggaatatcc ctagacgtct agcgatatcc atgccacgcc ttccccgttc
      301 acggtcgaat cgacccaaat ctcactcaag tccccaatga actcaaaaac agcaacctcg
      361 ccagccgcaa gcggcatgcc attgctgctg cttacatcac cggcgacacc acccacgtac
      421 atcgtaccgg tattggcagg caaggccttg accatcacag gacaattgac ctgcaagccg
      481 gtatgcaatc tctcggcagt gccggcggcg gtgacagttt tctgacctga aaaagtagca
      541 ggcatgggtt attttccttt ctttccagtt ttttgtgctc cagtaccacg tcctgtattt
      601 ggcttcgcag cccgctgccc atacaggtct tcggcggtga atttgagctt gcgaccatcg
      661 ggagcgatga ctacaatccc gccttgcggg gtctcgcgat agccgagaat aagcgctgct
      721 tcacagccca atatgctggc agccagctcc agcctagatt taccagattt tgcaaccatg
      781 agtgtcactc cgtaaccatc gcgccccagg cggagcgctc cgcctggggc tgggatcatc
      841 ttagatgcgc agggtgaaat tcgccctggc accgaaatac ttgaagacag atgtcgcggc
      901 ggcatcgcag gatagctccg cgtaaacctc atcgtcgtcg tccaaccaga tcggggtatc
      961 caggttgagg gtcatcttgt gctcatcgac atcgatgcgc tcagcagccg tatcgtgacc
     1021 cgtatcatag gtgaacgatt ggcttaccgg tgcggcaaaa gcagcgctat ccgccggcaa
     1081 ggcggccttg tagatggtag ccgcaacgga atccatggcg gcggtagcca cctcatacca
     1141 gatgttaata ctggccaact tgctaccctt gtaactggcg ctgttctggt tgagcttgat
     1201 cggaatcttg ataacgaacg cagcatcggc cgcgctgcgg ttcttgctcc acacattggt
     1261 agccacgcta tccgtccagg taccggcgga gaactgtgcc tcttccggcc ccaggaactt
     1321 gctcatggcg gtgtcgtgta tgtagcccat aatgttattc tcctgtttca agttcgaagt
     1381 tttaacttgg ttcggcggga taccatcccg acctgcatta cgccacattg ctcttatgca
     1441 atgggcggcc cgtctctgct ggcacagagt caggttacgc cacattgctc ttatgcaatg
     1501 ggcgaaagtc attgacccac acggcgataa aatggcggac tttcagccgg tgctcatcgt
     1561 tcatgaacac agcaggcgat agctcgctgc ccgctaagaa cacttcgggc atcaggccaa
     1621 agcgcgtgcc cacgaagatc gccggggcga tgcgcgggtc gcaggcagcc gcccagtcat
     1681 tggcgtcggt ccactcaggc accacgacca catcacccat ttcgccgcgc tgctggttct
     1741 cgctgtggat attggcggcg ttctcccaaa acgggtagag gatcttcttg gcggtcagct
     1801 ccagggcacg cggcaccaac aagtagcgcg ggttgatagc ctgcttgggt cccgtgccat
     1861 acacgcccgc ggcgttcttg atcagcatgg gctggttgta gacagcctgg gaaacagcct
     1921 cccactccgc ggtgctcaag gcggtcgtgc gcaggttggc atgacctccg gcggtagtca
     1981 cagcagtggc gttgaacaat gcctcgccat ctgccatcgt ggggccggtg ccgctgttat
     2041 cgctaaacaa tgcagccacc agtgctgaga ccttgcgcag cccagcattc gccagctcgc
     2101 gcgggtatgc gcgcagtttg cgggtctcgt cacggtcgat gagctccaac gtcaacggga
     2161 tataaccgcc gtacttggtg aagttggcgg tttcggggct gtcacccacg gttagctcgg
     2221 tatactcggc tccctccgcc accgttggca gggtgcccac cgtcccaatc agggtgccgc
     2281 tgatggattg caacgagctg aactgctcca cgctagaaat gcgctgccac caatcgtagc
     2341 cggcgcgccc tagctcaccc cactggttga ccacgatctt attgagggcg tttttaacca
     2401 gtccggcgaa atcagcggtg gtagccagcc tcaccctaga cggataatag ccgccatgca
     2461 actcttcatc gcctgtgagc atgagataca gctcgcggat gccggacagg cgagctacct
     2521 tcagcccttc catgccttta tccctgggcg cgcccattag gtcgtcaact gcggcttgta
     2581 gttgatcttc gctggaaaac acgccatgca cccggccagg cccaatcaca gcggagctgc
     2641 cggccaactg cgagaccagc tcgcgagccg aatcgataga ttgctgcagt tcatccggct
     2701 caaacagctt cccggcgaac tgcttacgga gatgctcctg cacagtagca ggcagcttcg
     2761 cagccgccag gccgctatcc agcagatact ggcacatctg caggcgtaac tgctgtgcgg
     2821 cttgccactc tttctgggaa gctgggattg agcgagcagc ctccagttca ttctgcgcca
     2881 aagattcacc cgccggggtc gcagcctgcc ttccgtcggg ggttggtgtg gtttcatcca
     2941 tcgcgatctt tgctcctttc tgtatgcccc cgctgggggg ttggctattt gtgccacgcg
     3001 tggcattaag tgcccggata aacgcgccgc cgcgcgccgg gttgataacc agatccagag
     3061 attcaaccct ggtaatccgt tcgacaacac gccccttgca ggtaaaccac aggtcagccg
     3121 aaaaccctac cctgggcttg ggcttcccct ctcgcagcat ctcccggccg agctgatcca
     3181 ccaatgggcc cgaaggtcct gaggtgacca gttccaccag aatgccgcgg cgctcctcgt
     3241 tccagcgagg gttgctgaac accccggcga gatcgagaac agaatggccc gaccaaaagg
     3301 cgtgatctac aaaacatgat gtcccgtccc agagagacag ggaggctctc aagacctcct
     3361 cggaaaattc ccaaccgttg ccatttccag cggtgatggc caagacttca aagccagcct
     3421 tagtatgttg tccaggacga gcggattgcg ctggccagcc ttcagcggcc aggatcaggt
     3481 gggtctcttg ctgttcgtct gacatacttt ctccttatta gatcgagcct gccgcagcgc
     3541 cccggcgaag catctcttcc acatcgacca cctcgccggc aaagcgatac gccatcctca
     3601 gcagctctgc atcgtcgatc aatccccgct ttctcagcag ggcaaaggca ctcacgatgg
     3661 tattggcagc cgcagccagg gaggtattgt cgcgagcgct gatatccgtc cctttggcgg
     3721 aaatgtatgc tctgggagca acgtgacgat ccacaagcgc cctgcgctgc acaaccaccc
     3781 tggccaggtc ttcgatcatc cacaaaaaga acttctgccg ttgctcataa tggcggaagg
     3841 tggggcctcc cgcggcttct gcggtagtac gggtggcgga ttctggctca gccaggaagt
     3901 gaaggggatt cccggatccg acggcgatca ttttcttgat agccaggccg tcctcagcag
     3961 cttcaaagct ggccagctcc gggttcagca cgctccaggt ctcgctctcg tcagccacca
     4021 ggatggcgcc cgggttaggc gggttggcat tgagctcagc ctggcgcgca agcctttccg
     4081 cctggctggt gaaagcggcc ttgaccacgt acaagaagct ttgcctgtag cggttcagcc
     4141 tggcgcgatc ctccaaccag gcggaataac gcgatagcca gcgcagcaag ggagccagat
     4201 cgctctctcc ccacttggca cccaccggcc ggttgacagc ataatgcagc acggctggcg
     4261 ggaagctgcc gtcggcatcc tggcggtcgg tgcgctcgtc atagacaggc caggcatgcc
     4321 ccataaagcc attgggggcg gcttgttcgg tgccatcgcc gcgcgggcgc tcccacacca
     4381 ccagctcctg ctcgatatcg ttgagggcgg tctcgatgtc aagaatatct tgggtgggca
     4441 aggtgcggat atatgacatg cccgcggcat cggtggaaac cacgatgaac agattacccg
     4501 tgcgggtcag ctcgtcacac aggtcgaacg ctctcaaagg catgcggttc agacgatggt
     4561 tccaccagtt ctgcaaaaag cggtgggtgc gctcgtgcct gcactccaca cccagcccac
     4621 ctccaacgac atattgagac attaattcga cgacacgacg ggcaagcgga ttttgtcgcc
     4681 aggcatccag ggcgtctcgc aggatctcat cgcggtcata gccatgccgg tcgcgggggt
     4741 aggtcgacac ataggtcgac caagacgcag ccaggcgatc cgtcttgtca tcgaggacgc
     4801 ggaccgccaa gctagcctgg cgttggattt ccttgccgaa aaagaagcca acaaagcgcg
     4861 aaagcatcaa aaaccctccc tgtccatttg cttgagcgga tccactgcct ggatgaccgc
     4921 ggttggggca gaaatacgcc actccaggct atccaggaca gcacacagcg ctgcagacag
     4981 gacccaatca tcgtgtatga gcttgccagc ctgccctggc aaactatcca agtcctgccc
     5041 tcccgacgtg ccttcaggaa ccccccactt catgcggcga tctgggccgg gtagcgtctc
     5101 catctggcag taggaaagtt gcaggttgaa ggcagaacgt tggctttctt cagtagcata
     5161 gtctttgaaa cgaccggttt ccacgatggt caagaaatcc cagcccaaat tgctcttcgt
     5221 ggccgcgttg aacacaaagg ggatgacccg gttgggaaaa gccctgtcaa gaaacgatgc
     5281 cagcccggcg cccaccccgc tagcgtcaat gacaatgtga cgggcaaccc aatgctctac
     5341 cagggctcta atctgggcat acaaggtgga gtgcctgacc ccgatccagc tccttcgatc
     5401 gacaacacga taggtagggg ctctcaaaac aggatctttc aagctagaga ggtctacctc
     5461 gaccacggta agggcggtag aatcacggcg cggattggca agactgcctg cgtgcaggcc
     5521 aacgtgagca tcagctcctt cgtccgcccg catagcctca tcctcaccag cgacgtcgat
     5581 cagcagggca tatatccgcc caggttgggc ggatggagcg gtctggcgtg ggtggctgcc
     5641 ctgcatgagg gccagtcggc gggcggggaa catgccccct tcggcgtcga tctcttcaga
     5701 atagtattgc gtgcggacca tgggatgatc ccgaccgagt ttggcaatct gctcagcaac
     5761 gaattttccg taggccggca cttcggcagc gacctcatcg gcggtgatga caaagactcg
     5821 ctgccggact ggacgccctg ccagtgcgtc cctgcgctcg gcttcacggg cggcgcgcag
     5881 ctcacgggcg agcagcgtgc ggctgttcca ggcggtgccc cagaaaacgc gcgtggcgtt
     5941 ggtggaagcg gccataggcg cgatgtcttt atcaaactta gcgaccaaca catcctgggc
     6001 ctcatcgact tccagcaggg ttgaagcggt ggcaccgacg atgttggctt cctgggatcc
     6061 agacaggaag aaaatgcgcg ctgttcccac gcgatagata tatccagact ctttcttcca
     6121 gttaaatttc gtgaaaaagt ttttagacag gacacgctcc aggcggcgca tggcgttgtg
     6181 ggattgaggc ttccaggtgg gagatatctt cacaatttca gcattggcca tgaagaaaag
     6241 catcaaaaga taggactcga cttgcgcctg cagctcgttt tttcctgact gccgaggaaa
     6301 catgaccaca atggtgtctc ctcgcccttc gatcaccgat tccacaatag ccagagcgac
     6361 ggattgctga taggagcgca ggcttagctt ggctgcgtgc ctagcgaaga gccgcacatc
     6421 acgcaccaag ctttttagtg taggcaagta ggttaacagc tcgttcactc gattcccaac
     6481 tccctggcaa cctcgatcac cgcctgcagc agcaccgcgt tcgcatctgt ggatgactta
     6541 tgcagctgtt tctgtgcctg cagcaaatta gccaggcggt tcatcgctga gccaatggtg
     6601 tccagcacct tcaacagggt ggtcagattc atgtcggggt ctgtagccaa ttgggaggtg
     6661 cgctgtacca ggttccgcag cgtgatgatc tcctcctcca ggtcggcttc cgcgccccgc
     6721 tcctgcaggc gtcgcgaata taagccatgc tttagggcat tcaggttgcc cggttgggca
     6781 ccgcgcttgc gccgtggccg ttcttctggc ttggtttcat cagcgttcaa attcatttcc
     6841 atctgcacat ccggccatga atcagagtcg attcttgaga aagtcatata cacccgaagc
     6901 agccaggcca gccgccagac caaagaaggc catctcaatc cagccggccc aatccggtgc
     6961 ggcctctcgc agccggtaag ccaccgccag cgccacgccc agtgccatgc tcaccgcgcg
     7021 caggctgttg cctcgcatgc cgaagccctt gatgtattcc actaacccaa agatcaagac
     7081 cagcaatgtg gttgggttca agacaaaacg atccaggtcc atacatgctc ctttctgcca
     7141 tttagccccc ccgctctccg gggtttccag aggcacagag accccggagg cgttaggagg
     7201 gatatccagt gtgcacgcgg agaacggcgc aaggttttct ggggaagtta aggcccgctt
     7261 cgcgtccacg caggattgca tgcgtatcgt agcaaaaaaa agtatttaga acaagtgttc
     7321 taacacgaaa tcccccgaaa tatcccccat ttttgcagac attttgagag ggttaaccag
     7381 gtcgaaggcg gtgagagcag cttactggct gggtgtgcgc agctttagcc gcatgcgcac
     7441 gcccgggtgc ggctccaaag tagcccccat gtgtggtcgc acatgccgct caaccagctg
     7501 caggtcgaag cgatgcaata tgatcgccag caccagcttg gcttccacct gaccaaaagc
     7561 cgcgccgatg caattgcgcg gtcccccacc aaacgccagc cacgtgtaag gtgttttctg
     7621 cccgccagag gcgtggcgct caggcaggaa gcgatggggg tcttgccaat ggccgcggtg
     7681 tcgctgggtg aggtagatag agtagatcac ccgctcgcca gccgggatat ggtatccctg
     7741 gtagtccagg tcagttgccg ccagacgcga gcccaggtgg atgggcgggt agaggcgcag
     7801 ggcttctcgt atcacgctat ccaggtagcg cagcccagac agttgctcaa tagtgacctg
     7861 acctgaaaag ttaaacgcat ctacctcgtc ttgggcgcgc tggtaagctt ggggatggct
     7921 gccaagcagg aatagtgccc agctcagcag cgcagtgctg gtatcgtgcc cggcgatgag
     7981 catggtgagc aattggtcgc gaatcaagtc gtcggtcatg cctgtggcga tcagggagcc
     8041 gagcaagtcc ggcgcgtcgc ggaagtcccc caaggcgcgc cgctcccgga tcaagcgata
     8101 gagataatca tccagccgct gcagcccgct tcgatactgg ggacgaggcg catcatgcca
     8161 taccatccaa atcccaggag agataaagcg gatactatcc aggatcggtt gccataaggg
     8221 ttgcagttca ggctgaaaat cggcgccaaa caatgtgccc accaggatca gcagggcaat
     8281 tttgcgcatc tcgaccagca tgtcaaccac gtcgccatcg tgccagtgtg atgtaacctg
     8341 atcaaccgcc tggctcatct ctgccaggta tctctccagc atgcgccggt gcagggctgg
     8401 gttcatcaat cgccgcaggt catcgtgggc ttgaccatct tccaccagca agccgtgacg
     8461 cagcagggct accacgggat cggcctcgtt ccgccacagc agctcgccgc gctcctcaac
     8521 caggacaaaa cgggccgcct gcggtccgac gagtacaacc ggggtgaaac ccggcagctg
     8581 gatgcgaaaa acatcgccca gctcgtcgtg gaagacctgc aacgcagcta aagggtgcct
     8641 ttcccgcagc atcgcctgca gggcgcgcag acccgtctga gaatccgggg taggtatggg
     8701 cctggatgct tccataccta aaagccgtcc tctagaagta gctcgctggc gacttttcca
     8761 gatagaatta ccatgggcac gcctccaccg ggatgcacag taccaccagc aaagtataag
     8821 cccttcacct gcggggcgcg gttgtgtggg cggcggaaag ccgcccagcg gctgttggac
     8881 gaagtaccgt atagagcccc acctcgagct ccactcatcc cctccaggtc ttcgggggtg
     8941 atgattccct gatattccag cttattacga acatcgaaac cacgccttgc aagctggtct
     9001 aagagacccc ctgcatacct ctcagcttcc cgcctccaat cccaggctgg acccagaggg
     9061 ggcgcattga ccagcacaaa ccagttctcg caacctggag gcgcgtcttc gggtgtggag
     9121 cgctgcgtga tcgacacata aattgtcggc tcttccggag gtataccctg cttgaatatt
     9181 tgctcgaact cccggcgata atccgatgag aaaaatatgt tgtggtgctt cagttgtggg
     9241 tgctcgccgc gcactcccag cagcaagata aagcccgagc aagatgatgg ggttttctgc
     9301 aaggacatca tccgcttacg gatagccggt tcaggtggca gcattttttc atagacggtt
     9361 gtcacatcca agttagcgat tactgccttt gcaggcaaat atgtgccatc cgccaggcgc
     9421 accccg
//
LOCUS       DBNGSX010000479         6384 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_533294, whole genome shotgun sequence.
ACCESSION   DBNGSX010000479 DBNGSX010000000
VERSION     DBNGSX010000479.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6384)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6384
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_533294"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..501
                     /locus_tag="AC16CB_14190"
     CDS             307..501
                     /locus_tag="AC16CB_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343667.1"
                     /translation="MAYSFKNSRGVTYYLHGKTRTLSSGKTTTLYYFAKAVQAGALDA
                     VPAGYVVSESKNGLPVLKRK"
     gene            complement(571..3168)
                     /locus_tag="AC16CB_14195"
     CDS             complement(571..3168)
                     /locus_tag="AC16CB_14195"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3343668.1"
                     /translation="MSTPFFLAQRNMRTRWGRTLLTLLGIVLGVAVVLAIQMTNQSTL
                     DSIRQVFDQATGQASLVVVSANRGADKLEEDTLFRAEKFQGVLAAAPSVRVQTLLASE
                     AKSWQIAFSINGIAAGNLLVLYGVDPDLDTQVRVYKLAAGRMPLSDEYEAILPEKYAT
                     EKKLAIGDELEILTPSGRDSLEIVGLLADEGVALLNDGVVTFAPIQVVQDLFAMGSGM
                     DEIGLRLENAISEDPAALESFKDRLNERLGRDAEVIYPAGRGQLVSQMMATYQLGLSF
                     FSVIAVFVGAFLIYNTFSMTIVERTREIGMLRSIGMPRRGIIQMVLAEAILLSSVGSL
                     LGIGAGIVLARGLMQLVSEVIIPVENLARVPLEGLVQSLGVGVGVTLGAALIPATQAA
                     RISPLAALRVRTRSIERVRAAVWLSGLALLIVGRITLYHITWPTEIRFTVGSTSILLI
                     LLGATLTVALASGWLERLARPLATVLYGREGALGSANVRRSVSRTALTVASLMVALTM
                     IISINSLAYSFKEDMTAWIVNALGGDLYVRSPIVMRESFGRQLASVDGVQVVTPTRII
                     LVRAAQRSVPRDQSVGDAIVLNAIEPSTFRQISDMEFAAGQGNTQANWQRLEQGDAVF
                     ISNVVADRYQLDQGDTLYLLTRRGEKGFYIAGIVMDFTGQGLVIYSTYLVLQRWFGET
                     GADRFTIKTAQGSSVAVVAEEIEGRYQKRRNISVQTTEEFKDNILNLMDRSFRLFDVL
                     NLIGVIIGALGVINTLTMNVMERQREIGGLRSLGMTRRQVLRMVLAEAQALGIMGGIY
                     GLGFGYAIAHVLILGTNLMIGYDLVFLFTAQPYIIGALIALVIVQMAAIYPARRAAHI
                     NIVEAIKHE"
     gene            complement(3165..3905)
                     /locus_tag="AC16CB_14200"
     CDS             complement(3165..3905)
                     /locus_tag="AC16CB_14200"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005239421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3343669.1"
                     /translation="MIVLETQQLTKQYQMGDVTVTALNDVNFAVERGEFVAIMGPSGS
                     GKSTLLHLLGGLDTPTSGEVVLAGQPLSQLSDDQITRFRRQKIGFIFQFYNLLPTLTA
                     AENVVLPLLIDGRTASEYKARLQVLLSLVSLEDRASHLPDQLSGGQQQRVAIARAFIN
                     DPEIVLADEPTGNLDSRSGKSILDLLRKACRELEATIVIVTHDPRAASYADRVVFLKD
                     GQIVHRLNNGTEGVSVNKIVEVMASLEL"
     gene            complement(3932..5119)
                     /locus_tag="AC16CB_14205"
     CDS             complement(3932..5119)
                     /locus_tag="AC16CB_14205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3343670.1"
                     /translation="MDLFLLTLSTIGLLVGLAITYWIHSQYRMEVVVRPSLSLSEINT
                     NPPLVSVIVPARNEDRNIRRCILALLAQDYPNFEVIVVDDRSTDETPRILADLQRSSA
                     NAHQRLIVIQGDELPTGWAGKPYALTQGAAVARGDWLCFVDADTFAIPELLSATYACA
                     QSQRADLFTMLTDQELGSFWEKSILPLVFTALSFGFPAARVNDPQKPDAIANGQFILI
                     RADVYHKLGGHEAVRGRIDEDKALAELVKRAGHRLVIADGRLVARTRMYTSLPEIWEG
                     WTKNIYLGLQDRLWLLLFGAVVGLLGALILPFWLLAGVVWLAAGGGIVAAIIAIQALI
                     VWGYLIAERMRVSRGMGISPLFAFTLPLGTLLFTAMMFVSAYNVLSGKGVTWKGRVYS
                     QSS"
     gene            complement(5143..>6384)
                     /locus_tag="AC16CB_14210"
     CDS             complement(5143..>6384)
                     /locus_tag="AC16CB_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HIR3343671.1"
                     /translation="IESLNSQGTDMGDRFRANQVLLADTKQRVSQLEAEIDILSKAEN
                     EQLVQTQNIARLDTQLENIQQAVQQWEAVGAARLAEITSSLQEDAFSPEARDRLAEID
                     AELKGIGYDAAAHDAARRKEADGRPADAAMRDLEKARAALSPLEREIADLQTQIKALK
                     DEFTRQQDEHESARASLDQAEAQAPDVETAESELLATQEQENQLRGDVGAAQQKVQVL
                     DTLKTRRKEYEAQREEIARQVGRYKQLERACGKDGVPALLIEQALPEIEGRANGILDR
                     LSGGSMSVRFVTQAAYKDRRREDLRETLDIQISDGAGTRDYEMFSGGEAFRVNFAVRL
                     ALSEVLAQRAGARLQSLVIDEGFGSQDAQGRQRLVEAINLVRQDFAKILVITHIDELK
                     DAFPTRIEVEKTDRGSTVRVT"
BASE COUNT         1389 a         1851 c         1768 g         1376 t
ORIGIN      
        1 gtatatatgt taaacgcctt aatctttgaa aggatgcgcg ctcagatgta ccacgcagtc
       61 tgggtactgg gattgcctcc atgaccccca attataatgg ttaagagagt cggagacgca
      121 tcggaacgac cttgaaaatg cgttcaccac cccttgaaag gggcaaattc aaccattgac
      181 gggctgaata aaacaagata aagtatagtt gtaaggtgtg agagaaggct atttggacta
      241 tctatcaatg aatcaatttt cactaaactg caatatagtt cgacagatat tggaaggagg
      301 ataatcatgg cttacagttt caaaaactca cgcggtgtaa cctattatct gcatggaaag
      361 actcgcaccc tttcgagcgg taaaacgacg acgctttatt atttcgcgaa agctgtccag
      421 gcgggtgcat tagacgccgt accagcaggg tacgtcgtat ccgaaagcaa gaacgggttg
      481 ccagttctaa agcgcaagta aaccaatcaa tctagttctc tgaaggaaag ggtggctcac
      541 aatgagccac cctagtttta gcgcgtatat ttactcgtgc ttgatcgcct cgacaatgtt
      601 gatatgcgct gcccgccgcg cggggtagat cgctgccatc tgtacaatca ctaaagcgat
      661 cagagcgccg atgatatagg gctgggctgt gaacaggaaa accaggtcgt agccaatcat
      721 cagattggta cccagtatca gcacgtgggc gatggcgtaa ccgaagccaa gtccgtagat
      781 gccgcccatg atgcccagtg cctgagcctc cgccagcacc atacgcagca cctggcggcg
      841 ggtcatccct aacgagcgca ggccgccgat ctctcgctgg cgttccatca cgttcatggt
      901 cagcgtattg atcaccccca gtgccccgat aatcacccca atcaggttaa gcacgtcaaa
      961 caggcggaag gaccggtcca tcaggttcag gatgttgtct ttgaactctt ctgtagtctg
     1021 tacgctgatg ttacgccgtt tttggtagcg cccctcgatc tcctccgcga ccacagctac
     1081 agagcttccc tgcgcagtct tgatcgtgaa acgatcggca ccagtttctc cgaaccagcg
     1141 ttgtaaaaca agataggtgc tataaatcac caacccttgg ccggtgaaat ccattacgat
     1201 cccggctata tagaagcctt tttccccacg tcgggtgaga aggtaaagcg tgtcaccctg
     1261 gtccagttgg tagcggtcgg caactacatt ggagatgaac actgcatctc cttgttccaa
     1321 tcgttgccaa tttgcttgcg tattaccctg accagcagcg aactccatgt cgctgatctg
     1381 gcggaaggtg cttggctcga tagcattcag aacgatcgcg tcacccacag attgatccct
     1441 cggcacagag cgctgcgcag ctcgtaccag gatgatgcgc gtgggtgtaa ccacttgcac
     1501 accgtccacg ctggcgagtt ggcgtccaaa agattcgcgc atgacgattg gcgagcgcac
     1561 gtacaggtcg ccacctaagg cattcacgat ccaggcagtc atgtcctctt tgaaggaata
     1621 tgccagcgaa ttgatgctga tgatcatggt caacgctacc atcagcgagg cgacggttag
     1681 cgctgttcga cttaccgagc gacgcacatt cgccgagccc agggctccct cccgcccata
     1741 taatactgta gccagcgggc gtgcgagccg ctccagccat ccgcttgcca gggccactgt
     1801 caaagtagcg cctagaagaa tgagcaggat cgatgtgctg cccacggtaa aacgtatctc
     1861 tgttggccat gtgatgtgat agagcgtgat ccgccctaca atgagcaggg ctagcccgga
     1921 gagccacact gcagcccgga ctcgttctat gctgcgggtg cgcactcgta gggccgccaa
     1981 tggcgagatg cgcgccgcct gcgttgccgg gatcagtgct gctccgagag tcactccaac
     2041 ccccacgcct aagctttgca ccagcccctc taggggcacc ctagccaggt tctccaccgg
     2101 aataatcact tcgctgacca gttgcatcaa gccgcgcgcc agcacaatcc ccgcgccgat
     2161 gcccagcagc gatcccacgc ttgaaagcaa tatggcctca gccagtacca tctgtatgat
     2221 gcctcggcgg ggcatgccaa ttgaccgtag catgccaatt tcccgcgtgc gctccacgat
     2281 ggtcatggag aatgtgttgt agatcaggaa ggcacccaca aacacggcga tgacggagaa
     2341 gaagctcaag cctaattggt aggtggccat catctggctg acaagctgcc cgcgacctgc
     2401 agggtagatc acctcggcat cccgccccag gcgttcgttc aggcggtctt tgaacgattc
     2461 cagggcggct ggatcttcgc tgatggcgtt ctccaagcgc aagccgatct cgtccatccc
     2521 gctgcccatt gcaaacagat cttgcacaac ctgtataggc gcaaaggtta ccaccccgtc
     2581 attgagcagc gccacgcctt cgtcagccag caagcccaca atctccaggc tatcgcgtcc
     2641 cgatggagtc aagatctcaa gttcatcacc tatcgcgagc tttttctctg tggcgtattt
     2701 ctccggcaag atggcttcgt attcgtcaga gagcggcatg cgtccagcgg caagtttata
     2761 cacacgtacc tgagtatcga ggtcggggtc cacgccgtaa agcacgagta aattgcctgc
     2821 ggcgatgcca ttgattgaaa aggcgatctg ccaggatttg gcctcgctgg ctagcaaggt
     2881 ttgcacgcgc acggagggtg ccgctgccaa cacgccctga aacttctcag cgcggaagag
     2941 cgtgtcttcc tccagtttgt ctgcaccacg gttggctgat accaccacca ggctggcctg
     3001 tccagtcgcc tggtcgaaca cctggcggat cgaatccagg gtgctctggt ttgtcatctg
     3061 gatggccaaa acgaccgcca cacccagcac aatgcccaac agagtaagca gcgtgcgccc
     3121 ccaccgcgtg cgcatattcc gctgagcaag gaagaaaggt gtactcatag ctccaaagac
     3181 gccatcactt caacaatttt attgacggac acaccttctg tgccattgtt caggcggtgc
     3241 acgatttgcc catctttgag gaataccacc cggtcggcat aggaagcggc gcgcggatcg
     3301 tgggtgacga tgacgatggt cgcctccagt tcacggcaag ccttgcgcag cagatccagt
     3361 atggacttgc ccgagcgcga gtcgagattg ccggtgggct cgtcggccag cacgatctcc
     3421 ggatcattga tgaaggcacg cgcaatagcc acccgctgct gctgcccacc ggaaagctgg
     3481 tcgggcaggt ggctggcgcg gtcctccagg ctcaccagcg ataacagcac ctgaaggcgt
     3541 gctttgtatt ctgacgccgt ccgtccgtct ataagcagcg ggagtaccac gttctcggcg
     3601 gcggtcagcg tgggcaatag gttgtaaaac tggaagatga agccgatctt ctgccggcga
     3661 aagcgggtga tttggtcgtc actcaattgc gagagcggct gtcctgccag gacgacctcg
     3721 cctgaagttg gcgtatccaa gccgcccagc aggtgtagca aagtggattt cccagagcct
     3781 gatggcccca tgatggcgac gaactcgccc cgctcaacag caaagttaac atcgtttagc
     3841 gcggtgacgg tcacatcccc catttgatac tgtttggtaa gttgttgagt ctcaagaacg
     3901 atcattggac aataaattga tgcagtttta tttatgagct ttgggaatat acccgtccct
     3961 tccaggtgac ccccttgccc gaaagcacgt tatatgcgga aacaaacatc atggctgtga
     4021 agagcaatgt ccccagcggc aaggtgaagg caaataaggg agagatgccc atacctcgtg
     4081 aaacccgcat tcgctcggca atcaggtaac cccagacaat cagagcctgt atggcaatga
     4141 tagccgccac gatgcctccc ccggcggcaa gccagactac tccggccagc agccaaaaag
     4201 gtaggatcag cgcccccagc agcccaacca ccgccccgaa aagcagcagc cacaggcggt
     4261 cttgcaagcc aaggtagatg tttttcgtcc aaccttccca aatttcaggc agcgaggtgt
     4321 acatgcgcgt gcgcgcaacc aaccgaccgt cggcgatcac caggcggtgc ccggctcgct
     4381 taaccagttc agccagggct ttatcctcgt cgatgcgccc gcgcacggcc tcgtgcccgc
     4441 ctagcttatg gtaaacatca gccctgatca ggataaactg cccgttggct atagcgtcgg
     4501 gtttctgcgg gtcattgacg cgagccgcgg gaaagccgaa agacagtgct gtgaagacca
     4561 gtggcaggat agatttttcc caaaagctgc ccagctcctg gtcagtcagc atcgtgaaca
     4621 ggtcggcgcg ctggctttgt gcacaggcat atgttgcgga gagtagttcg gggatggcga
     4681 aggtatctgc gtccacgaag catagccagt cgccgcgcgc tacagcagct ccctgagtca
     4741 gggcatatgg tttgcctgcc cagcctgtag ggagctcgtc accttgaatc acaatcaggc
     4801 gctgatgggc gtttgccgag gagcgctgca ggtctgcaag gatgcgcggc gtttcgtcgg
     4861 tcgagcggtc gtccaccacg atgacctcaa aattcgggta atcctgtgcc agcaacgcca
     4921 ggatgcagcg acggatgtta cgatcctcgt tgcgggctgg cacaattacg gataccaaag
     4981 gtgggttggt gttaatctct gagagggaga gcgatggacg gacaacgacc tccatgcggt
     5041 actggctgtg gatccagtac gtgatcgcca gaccgaccaa taatccgatt gtggaaagtg
     5101 tcaagaggaa aaggtccatt ggtaatacag taaatgcgtg ctttatgtca cacgcacagt
     5161 cgacccccta tcggtcttct ctacctcgat acgtgtggga aacgcgtcct tgagttcatc
     5221 gatatgggtg atcaccagga ttttggcaaa atcctgacgc accaggttga tagcctccac
     5281 caggcgctgg cggccctggg cgtcctggct gccgaaaccc tcgtcgatca ccagggattg
     5341 caagcgggct ccggcgcgtt gtgccagcac ttcggagagc gccaggcgga cggcaaaatt
     5401 gacgcggaaa gcctcgccac cggaaaacat ctcgtaatcg cgcgtcccag caccatcaga
     5461 gatctggatg tccagggtct ccctcagatc ctcccggcgg cgatccttgt aagctgcctg
     5521 ggtgacgaag cgcaccgaca tgctacctcc gcttaggcgg tctaggatgc cattggcgcg
     5581 cccctcgatc tcgggtaggg cttgctcaat aagcagggct ggcacgccat ccttgccgca
     5641 ggcgcgttct agttgtttgt agcgacctac ttgccgggcg atctcttcgc gttgtgcttc
     5701 gtattccttg cggcgcgtct tgagtgtatc cagtacttgc accttttgct gcgccgcgcc
     5761 cacatctccg cgcagctggt tctcctgctc ttgggtagcc agcagctcac tctcagcggt
     5821 ttccacgtcg ggcgcctgcg cttcagcttg atccagcgag gcgcgtgcgc tctcatgttc
     5881 atcttgctgg cgggtgaact cgtcttttag ggctttaatc tgcgtctgca ggtcggcgat
     5941 ctcgcgctcc agtggagaga gggcggcccg cgccttctca agatcgcgca tagccgcgtc
     6001 cgctggacga ccgtcagcct ctttgcggcg ggcagcgtcg tgagccgcgg catcgtagcc
     6061 aatacccttg agctcagcgt cgatctcggc taatcggtcg cgcgcctcgg ggctgaaagc
     6121 atcttcttgc aggctagagg ttatctctgc caggcgcgct gctccaaccg cctcccattg
     6181 ctggacggct tgctggatgt tctctagctg ggtatccagg cgggcgatgt tttgggtttg
     6241 cacgagctgc tcattttcgg ccttgctaag gatgtcaatt tccgcctcaa gctggctgac
     6301 ccgttgtttg gtgtctgcaa gcaaaacctg gttggctcgg aagcggtctc ccatgtctgt
     6361 gccctggctg ttcaagcttt cgat
//
LOCUS       DBNGSX010000480         6902 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1433238, whole genome shotgun sequence.
ACCESSION   DBNGSX010000480 DBNGSX010000000
VERSION     DBNGSX010000480.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6902)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6902)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6902
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1433238"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="AC16CB_14215"
     CDS             <1..200
                     /locus_tag="AC16CB_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343672.1"
                     /translation="LCMPQPGFDLVCRYFGAQYSTFTFRILVKEPKSSPFSLLSGEKS
                     PKCDCPITDAVVIQDMGGNAM"
     gene            584..1789
                     /locus_tag="AC16CB_14220"
     CDS             584..1789
                     /locus_tag="AC16CB_14220"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR3343673.1"
                     /translation="MATKSITQRPSFNDMFYSSLFKLPEGQRMQACLQCGTCSGVCPF
                     GYLMEYPPGKMIAALRAGLFERVINYESLWMCVSCYACTQVCPAKIPLTPGLLTRAKE
                     EMVLAGNVPSELQDALENSQRYGNPLGESPRKRAMWARGIDPPARIMSQVGGPVDVLW
                     FVGDYASFHPRVQMVSQAVAQVFNVIGVDFAILGPEEFSDGDSQRLAGERGLFEMLAE
                     KNGQVFNKYTFSQIVTTDPHAYNALKNEYPTLGIVYPVKHYTQFLVDRLDQLKPHLRE
                     EIQARVTYHDPCYLGRANGEYDAPRRLLEAIPGVKLVEMTHHMETGLCCGGGGGGMWL
                     DGFTWEKAHVRLSEWRVREAVQAEAEILAVACPYEPPRFEDAIKMIEKAKRLVVRDVA
                     ELLAESMGE"
     gene            1812..2576
                     /locus_tag="AC16CB_14225"
     CDS             1812..2576
                     /locus_tag="AC16CB_14225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR3343674.1"
                     /translation="MKIVVLIKMVPDLVEELSISPSGIELDTTWLRLKINEFDDHAIE
                     QAILLKEQYGGEVTVLAPEMEGVDDALFTAAAKGADRLIQLSGKFDNSWNNHALARAF
                     LPLAQELQPDLVLTGVQAHNDLDGSLGPLVAALMGLPYVGYVAGVTVSDGVATIRKEY
                     PAGLLAEMEVILPAVLGIQAAEQPPRYVAFSKVRQSMKTTTLDERPAGESDPQGGVTV
                     EHMFQPETGEGATMLEGDLTQVTDKLVSILHELDVI"
     gene            2601..3581
                     /locus_tag="AC16CB_14230"
     CDS             2601..3581
                     /locus_tag="AC16CB_14230"
                     /inference="COORDINATES: protein motif:HMM:NF013200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR3343675.1"
                     /translation="MNQDVFVSIEHLRGQVTDLSYGMLAAARQVAAEAGGEVVAVLLG
                     HTGQHLAQGLAADRVLSIDHPALADFTSEAYIQAWSYLLREYNPRLALFGDTSIGADV
                     AGGLSIRMGLPLVSACQVLLVENGELLFRSQICGGKIMAQGKLPPTTALVTMVPGGYR
                     AEEARGEEPSDIISLEAPPLEDLPVRLKEYIEPEISDVDITKENVLVAVGRGIQNQDG
                     IELVQELAEALGGVVCASRPVIDQGWLPITRLVGKSGGQVKPVVYLALGISGAPEHVE
                     GIVDSQVIIAVNTDLAAPIFNVAKYGLTCDLFDLVPILVDKIRLSRSIQV"
     gene            3604..5589
                     /locus_tag="AC16CB_14235"
     CDS             3604..5589
                     /locus_tag="AC16CB_14235"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HIR3343676.1"
                     /translation="MPIRETFWNIPQWAEIGQYILGLLSLLILIYGVVQRIRRWRQGQ
                     PENRSDQLLLRLKSLWVEGVLQARVTSDIYAGVMHLTIFWGMVVLFLGTVVATVDWDV
                     THLFFGFQFLTGGIYVIYELVLDIFGILLLVGLGMALYRRYIVRPGRLQNMPAGRLAW
                     DDAYVLTMLVLITVSGYLIEGLRIAVIRPDWAAWSPVGSAIASAFLALGDPTSRSLHL
                     SLWTAHGLLSFMFIASVPYTKLFHLISTPVNIFFQSTKPVGALVPVRYSSGPGVQEWK
                     NFTWKQILDFEACTRCGRCHDACSALGSCGLLSPRDLMIKLDQHVWERQNGKSLHGDV
                     LSPEEIWACTTCRACSQVCPAYIDHVTTLVDLRRHLVDEGEMDGLLQEALTNLGRYGN
                     SFGQSERMRARWTAGLEPAIKDIRKEPAEYLWFVGDYASYHAGVRDITRKTAEVFQKA
                     GIDFGILYEAERNSGNDVRRVGEEGLFEMLVEKNLAVLDRCDYQSVVTTDPHSYNTLK
                     NEYSTNGNGRHPVLHYSELLDRLLTAGQLKLSRKLDYTVTYHDPCYLGRYNGVYEVPR
                     RVISATGCKLVEMPRHGDRALCCGAGGGRIWMEEGPVLERSSEARIHEAVGQNGVQVL
                     VTACPKDVAMYTDAVKTTGFADRLMVKELVELVHEAL"
     gene            5604..>6902
                     /locus_tag="AC16CB_14240"
     CDS             5604..>6902
                     /locus_tag="AC16CB_14240"
                     /inference="COORDINATES: protein motif:HMM:NF024842.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3343677.1"
                     /translation="MNEKVGVYICHCGTNIAGTVDVMEVARWVSEQPQVAIAREYKFM
                     CSSLGQELIENDIKEMGLTRVVVAACSPHMHEKTFRSACQRAGLNPFLFEMANIRELD
                     SWITDDKEAATQKAKAMIKGAVERVLHHQPLNYLPVNINPHTLVVGGGIAGITSALEL
                     ANAGHHVYLVERDPSIGGHMAQLDKTFPTLDCAACILTPRMVEVGQHPNITLLSYSEV
                     ENIEGYMGNFNVTVRKKARYINEELCTGCAVCEEKCPKKVLDEDFNVGLGYRKAVYRP
                     FPQSVPMYPVIDVQNCTYFIKGKCKACEIFCPAGGIDFDQKDELLELQVGSIILATGY
                     SPFDARRIPQYGYGRLPNVYTSVEFERLVNASGPSAGRIVLRDGVTTPESVAIIHCVG
                     SRDQNYNEYCSRVCCMYSLKFAHLVRERLPDAEVYNFYIDI"
BASE COUNT         1570 a         1624 c         1979 g         1729 t
ORIGIN      
        1 tgctgtgcat gccgcagcca ggatttgacc tggtttgtcg ctattttggg gctcaatatt
       61 caacctttac ctttcggatt ttggttaaag agccaaagtc cagcccattt tctctcctca
      121 gtggtgaaaa atcgcccaaa tgcgattgcc ctatcactga tgcggtggtc atacaagata
      181 tgggagggaa tgctatgtag gggctagaac actgtttatg ccaaagatat gataatgtga
      241 taaaaatcac aaaccgaata tgcgctttgt tactggttta tttgtgatat atttcacata
      301 ataaagatga cataatacat gccccatatc agcgcgagct ccctagaacg cacacttggc
      361 agagtatctc ggtgatcgtt tctgcggttt cgttgtcgct ttcataacac accacacatt
      421 ccagccttga aaactgaata gcataggcaa aatttgtcta aatccttttc agcacgattc
      481 tcattcgcgt gctcatggat tggtttcgcc aagctttagg ccgggggagt tttacctatt
      541 tatatctatt ttgcctggcc tagtgtaagc aggaggtgtt gctgtggcta caaagtccat
      601 tacccaaaga ccttccttca atgacatgtt ctattccagc ttgtttaagt tgccggaagg
      661 tcagcgcatg caggcttgcc tgcaatgtgg aacctgtagc ggagtatgcc cctttggcta
      721 tctaatggaa tatccgcctg gcaaaatgat cgctgcactg cgcgccggtt tgtttgagcg
      781 ggttataaac tatgaatcgc tctggatgtg cgtctcatgc tatgcctgca ctcaggtctg
      841 cccagcaaaa ataccgctca ctcccgggct attgacacga gcgaaagaag aaatggttct
      901 ggctggcaat gtccccagcg aattgcagga tgcccttgag aattcccaac gttatggtaa
      961 cccgctcggc gaatctccaa ggaaaagagc catgtgggct agggggattg atccgccggc
     1021 gcgcatcatg agccaggtag gaggacctgt agatgtcctc tggtttgttg gcgactacgc
     1081 ctccttccat ccgcgcgtcc aaatggtcag ccaggctgtg gcccaagtgt ttaatgtcat
     1141 cggagtggat ttcgccatat taggacccga agaatttagt gatggcgatt ctcagcgatt
     1201 ggcaggtgag cgcggattgt ttgagatgtt ggctgagaaa aatgggcaag tctttaacaa
     1261 atataccttc agtcaaattg taacgaccga cccgcatgca tacaatgcac ttaagaatga
     1321 atacccaacg ctggggatcg tctacccggt gaagcattac acccaatttc tggttgatag
     1381 actagatcag ctcaaaccac acttgcggga ggaaattcaa gcaagggtta cataccacga
     1441 tccctgctac ctgggcaggg caaatggcga gtatgacgcg ccgcgccgct tgctggaagc
     1501 cattcctggt gtgaagctgg tggagatgac tcatcatatg gagacaggtc tgtgctgcgg
     1561 gggtggtgga ggtggtatgt ggctggacgg gttcacttgg gagaaagcac atgtgcgcct
     1621 ctccgagtgg cgcgtccgcg aggcggtgca agccgaggca gaaatactgg cggtcgcctg
     1681 cccctacgag cctccgcgct tcgaagatgc catcaagatg attgagaagg ccaaacggct
     1741 ggttgtcagg gatgtcgctg agttgctagc cgagtcgatg ggtgaatgag ctcttgagag
     1801 aggagcagac gatgaagatt gtagtcctca tcaaaatggt gccagacctg gtggaagaac
     1861 ttagcatctc cccaagtggg atagagcttg atactacctg gctgcgcctg aagattaatg
     1921 aattcgacga tcacgctatc gagcaagcta tcttgctcaa agaacagtat ggtggtgaag
     1981 tgacggtact ggctcctgaa atggaagggg ttgatgatgc gctattcaca gctgctgcca
     2041 aaggggccga ccggctgatc cagctcagtg ggaagttcga taatagctgg aataaccacg
     2101 ctctagcgcg cgcttttcta ccattagcgc aagagctaca gcctgacctg gtattaaccg
     2161 gtgtgcaagc tcacaacgat ctggatgggt cgcttggacc cctagtggca gccttgatgg
     2221 gattgcccta tgtgggctat gtggctggtg tcaccgtaag cgacggggta gccaccatcc
     2281 gtaaggaata tcctgccggg ctcctggcag aaatggaagt gatcctgccg gctgtattgg
     2341 gtattcaggc ggcagagcag ccaccgcgct atgtagcctt tagcaaggtg cgccaatcga
     2401 tgaagaccac cacactcgat gagcgcccgg caggagaatc agacccgcaa ggtggagtga
     2461 ctgtggagca catgttccag cctgaaactg gcgaaggggc aaccatgttg gaaggcgacc
     2521 tcacccaggt tacagataag ttggtttcca ttctccacga attggacgtg atctgattcg
     2581 ctctgaagag gagtttagcg atgaaccagg atgtatttgt ttcgattgaa cacctgcgtg
     2641 gtcaggtgac tgatctctcg tatggaatgc tggcagccgc tcgccaagtt gccgctgaag
     2701 cgggtgggga ggtagttgct gttttgctag gccatactgg tcaacatctg gctcagggat
     2761 tggctgctga tcgtgtgctg tctattgacc atccagctct ggctgatttt acgtcggagg
     2821 cgtacataca agcctggtct tacttgcttc gagaatacaa tccgcgcctg gcgttgtttg
     2881 gcgacacgtc aattggggcg gatgtggctg gtggactttc aattcggatg gggttaccac
     2941 tggtgagtgc ttgccaggta ttgcttgtgg agaatggaga gttgttgttt cgcagccaga
     3001 tctgtggcgg caaaatcatg gcacagggta aattaccccc aaccaccgct ctggtaacaa
     3061 tggtccccgg tggttatagg gcagaagagg cgcgtggtga agaaccctcg gatattatct
     3121 ctcttgaggc cccgcctctt gaagacttgc cggtacgcct caaagagtac atcgaacccg
     3181 agattagcga tgtggatatc accaaggaga atgtgctggt ggcagtgggg cgcggtatcc
     3241 agaaccaaga tggcatcgaa ttggtgcaag agttagctga ggccttaggt ggtgtggtat
     3301 gcgcttcacg cccggtaatt gatcagggtt ggctgccgat aaccaggttg gtgggcaaat
     3361 ccggcgggca agtcaagccg gttgtgtacc ttgcgctggg aatcagcgga gcgcctgagc
     3421 atgtcgaggg catcgttgat agccaagtga tcatcgccgt caatactgac ctggctgcac
     3481 cgatcttcaa cgtagctaaa tatggcctca cgtgcgatct atttgatttg gtgccaatcc
     3541 ttgtagacaa aattcgtcta tctcgttcga tccaagtatg agctgggagt tcaggttaaa
     3601 accatgccga ttcgtgagac cttctggaac attcctcaat gggccgagat tgggcaatat
     3661 attcttggct tgctttcgct gctgatactg atctatggtg tggtccagcg cattcgacgc
     3721 tggcggcagg ggcagccgga aaatcgctct gaccagctat tgcttcgctt gaaatcgctg
     3781 tgggttgagg gggttttgca agcgcgtgtt acgagcgata tctatgccgg ggtcatgcat
     3841 ctcaccatct tctggggcat ggttgtgctt ttccttggca ctgtggtggc gacagttgat
     3901 tgggatgtaa cccacctgtt ttttggcttc cagtttctga caggtggcat ttatgtgatt
     3961 tacgagttgg ttctggacat tttcggtatc ttgttgttgg ttggactggg gatggcccta
     4021 tacaggcgat atattgtccg tcctgggcgc ttgcaaaaca tgcctgcagg tcgcctggcc
     4081 tgggatgacg cctatgtgct taccatgtta gtgttgatca cggtcagtgg ttatctgata
     4141 gaggggctgc gcatagccgt catcaggccg gattgggccg cctggtctcc ggttggcagt
     4201 gcaattgctt ctgcgtttct ggctttaggt gacccaacga gtcgcagcct gcaccttagc
     4261 ctctggactg cgcacggctt gctgtctttc atgtttattg ccagcgttcc ttataccaaa
     4321 ctctttcatt tgatatcgac accagtcaac atcttcttcc agtccacaaa gccggttggt
     4381 gcgctggtgc cagtacgcta ttcctccgga ccgggtgttc aagaatggaa gaacttcact
     4441 tggaagcaga ttctggattt tgaggcctgc acccgctgcg ggcgctgcca tgacgcttgc
     4501 tcggccttgg gcagttgtgg attgctctcc ccgcgcgatc tgatgattaa gctggatcag
     4561 cacgtatggg agcggcaaaa tggtaaaagc ctgcatggtg atgtgctctc acccgaggag
     4621 atatgggcat gcaccacttg ccgggcctgt tcccaggtct gcccggctta tattgaccat
     4681 gtgactaccc tagtagacct gcgccgccat ttggtggatg aaggcgagat ggatggcttg
     4741 ttgcaggaag ccttgaccaa cctgggtcgc tatggcaact cctttggtca atcggagcgc
     4801 atgcgcgccc gctggacggc aggtttggag cctgcgatca aggatatccg taaagagcca
     4861 gcagaatatt tgtggtttgt gggcgattac gcttcttacc atgccggcgt gagggatatc
     4921 acgcgtaaga ccgctgaagt attccaaaaa gcaggcatag atttcggtat tctctacgaa
     4981 gccgaacgca attcaggcaa tgatgtgcgt cgtgtgggtg aggagggcct atttgaaatg
     5041 ctggttgaga agaacctggc tgtactcgac cggtgcgatt atcaatctgt cgtaaccact
     5101 gacccgcaca gctacaacac actcaagaat gagtattcga cgaatgggaa tgggcggcat
     5161 ccagtattgc attacagcga actgcttgat cgtttgctca ctgcggggca gctcaagctg
     5221 agccgtaaac tggattatac ggtcacctat catgacccat gctatctcgg ccgatacaac
     5281 ggtgtatatg aggtgccgcg ccgagtgata tctgcaactg gctgcaagct ggtagaaatg
     5341 ccacgtcatg gcgaccgggc attgtgctgt ggggctggtg gtggtcgcat ttggatggaa
     5401 gaaggaccag tgctagaacg ctcaagcgaa gcccgcatcc atgaagccgt ggggcagaat
     5461 ggtgtgcagg tgctggtaac ggcttgcccc aaagatgtcg ccatgtacac agacgcggtc
     5521 aaaaccactg gcttcgcaga tcgtctgatg gtcaaggaat tagttgagct ggtacacgaa
     5581 gcgctctaaa caggagggca gcgatgaatg aaaaagtagg cgtatacatc tgccattgtg
     5641 gaacaaatat cgctgggacg gtggatgtga tggaggttgc acgttgggtc agcgagcaac
     5701 cccaggtagc cattgcccgc gagtacaaat tcatgtgctc cagcctaggc caagagctga
     5761 ttgagaatga tattaaagaa atggggctta ctcgcgtcgt ggtggcggcc tgttcacccc
     5821 acatgcacga gaagactttt cgtagcgctt gccagcgggc tggtttgaac ccgtttttgt
     5881 ttgaaatggc gaatatccgt gagctggatt cgtggatcac ggatgataaa gaggccgcca
     5941 cccaaaaggc taaagcaatg attaagggcg ctgtggaacg tgtgttacac caccagccgc
     6001 ttaattactt gccggttaat atcaatcccc ataccctggt tgtgggcggg ggcatcgccg
     6061 gcattacatc tgctttggaa ttagccaatg cggggcacca tgtctatttg gtggagcgcg
     6121 atccttcgat tggcggtcat atggctcaac tcgataagac ctttcctaca ctggactgcg
     6181 ctgcctgcat cctgacacct cgcatggtgg aagtggggca acatcccaat atcaccttgt
     6241 taagttacag cgaggtggaa aatattgaag ggtatatggg aaacttcaac gtcacggtgc
     6301 gtaagaaagc ccgttacata aatgaagaat tgtgtacggg ttgcgcggta tgtgaagaga
     6361 agtgtcctaa gaaggtgctg gacgaagact tcaatgttgg cctggggtat cgtaaggcag
     6421 tataccgccc gtttcctcag tctgttccca tgtacccggt cattgacgtg cagaattgta
     6481 cgtactttat caaggggaag tgtaaggcgt gcgagatctt ttgtcctgct ggcgggattg
     6541 acttcgatca aaaggatgaa ctgctggagc tacaggtggg aagcattatc ctggccaccg
     6601 gatatagccc gttcgatgcc cgccgtatac cccaatatgg ttatggtcgc ctgccaaacg
     6661 tatataccag cgtcgagttt gagcgcctgg tgaacgcatc cgggccatcc gcaggaagga
     6721 tcgtcttgcg agatggagtg accacgcctg aaagcgtagc catcatccac tgcgtgggct
     6781 cacgcgacca gaattataac gagtactgtt cccgcgtatg ctgcatgtat tctttgaagt
     6841 ttgcccacct ggttcgcgag cggctaccgg atgcggaagt ttataacttc tatattgata
     6901 tt
//
LOCUS       DBNGSX010000481         8961 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1779338, whole genome shotgun sequence.
ACCESSION   DBNGSX010000481 DBNGSX010000000
VERSION     DBNGSX010000481.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8961)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8961)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8961
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1779338"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..477
                     /locus_tag="AC16CB_14245"
     CDS             160..477
                     /locus_tag="AC16CB_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014676587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HIR3343678.1"
                     /translation="MKRVGFQFKVRQDRLADYKEQHKHVWPEMLDALREAGWHNYSLF
                     MRSDGLIFGYFETDESLAVAQAKMAAKEVNTRWQKFMSHFIDANARPDETFLELEEYF
                     HLD"
     gene            570..1844
                     /locus_tag="AC16CB_14250"
     CDS             570..1844
                     /locus_tag="AC16CB_14250"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019318 - hexose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HIR3343679.1"
                     /translation="MNQQPTTTQIEQAYKQARERYAAVGVNTEEALARLADIAISLHC
                     WQGDDVVGFEDPDRGLSGGIMTTGNYPGKARTPNELRQDLDKVYSLIPGTHRLNLHAI
                     YLETDNKVPRNEIEPKHFARWVDWAKENNHGIDFNPTCFSHPLADDGFTLSHPDVGIR
                     QFWIEHCIASRVIGAHFGRELGTPAITNIWIPDGYKDTPVDRLGPRRRLRDSLDRILT
                     EPTDRRYNLDSVESKLFGIGSESYVVGSHEFYLGYATANQTLLCLDAGHFHPTESIAD
                     KISAVLLYVPELLLHISRGVRWDSDHVVTLTDELQAMMGEIVRGNYLDRVHIGLDFFD
                     ASINRIAAWAIGARNALRALLMALLEPTEQMRALEMAGDFTARLALLEELKGMPLGAI
                     WDSYCLQQNVPVGMTFLDEIRAYENKVLAQRG"
     gene            1847..3304
                     /locus_tag="AC16CB_14255"
     CDS             1847..3304
                     /locus_tag="AC16CB_14255"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HIR3343680.1"
                     /translation="MPATTVLAIDIGAESGRVMAVHFDGKQLASEELHRFPNTTITVN
                     RTLHWDFLRLWGDIQEGIEKGKALNPASIGVDTWGVDFGLLDSQGNLIGNPVHYRDGR
                     TAHMMDIAFARVPQADIFAQTGIQFMPINSLYQLLSLVESQSPQLQLADTFLTAPDLL
                     NYWLTGVKVCEFSNATTTQLFNPTTGTWATGIMAKLGIPSHIFPEIVPPGTRLGLYEG
                     IPVIAPACHDTGSAVAAIPTQRPDFAYISSGTWSLVGLEVNSPILTPEALTANVTNEG
                     GVYGTYRLLKNVMGLWILQQCRATWAAEGEVYTYAELVNMAAQAEPLATGFDPNDPAF
                     LSPGDHPQLIRDLCSKTNQPVPETPGAVVRSVLESLALAYREVLELVSAVANRHVSVL
                     YIVGGGAQNQLLNQMTADATRLPVIAGPIEATVIGNALVQLITLGKITDISQARQILA
                     NMAELKQYEPVGNAGSKTSVWEDAYDRYRRNRPPR"
     gene            3358..3663
                     /locus_tag="AC16CB_14260"
     CDS             3358..3663
                     /locus_tag="AC16CB_14260"
                     /inference="COORDINATES: protein motif:HMM:NF041918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HIR3343681.1"
                     /translation="MKIKLFGGLRQKAGSKEQTASGATIREALEGICLDNETLRAAIF
                     DGNVLHPHVRVMVNGRDCELAQGLDTPVSDDDQIAVFPPIAGGQCTRRGGTEKMRSG"
     gene            3781..5580
                     /locus_tag="AC16CB_14265"
     CDS             3781..5580
                     /locus_tag="AC16CB_14265"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004066796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HIR3343682.1"
                     /translation="MQGWTGKILDINLTDGSIETLPLDMEMARLFLGGRGLGARLLWD
                     LVGPEVSPLSPENVLIFTTGPITATGSQTSNRFNVSTKSPLTDTILHANSGGWWGMQF
                     KRTGYDALIVRGKSKTPVMIEIKPEGVELKEAGHLWGKGVFETTEALGQSRNQRNVLC
                     IGPAGENQVRIAAIMNDKERALARGGPGAVMGSKNLKAIVVEGKEKNQPADQELFKFM
                     LYETGKLLKASPLTSQGLPEFGTAVVMNVVNEIGALPTRNFQQSQFEGADKISGEAIT
                     DSILVKNQACWACPITCTRITKTDSGKEGEGPEFESAWAFGAQCGIDDLDTITEANYL
                     CNDMGLDTISMGNTIGCAMELAEKGLIANEFGFGQAGKLLDLISDTAYRRGLGAEMAE
                     GSYRMAEKHGAPELSMSVKKLEMPAYDPRGMQGQGLVFATGNRGACHETGNMLGPEVL
                     ALPRLIDRFATQGKAGIVSVHQNSAAVIDSLVYCKFTNMAVAEEFFARTLSAVTGEHF
                     TADDLMMVGERVWTLERLYNLREGFTKKDDTLPDRMLSEPVPDGPSKGFTVKLAPMLE
                     EYYAFRGWDQNGVPKPEKLKELSLEFAIEEAHD"
     gene            5573..6184
                     /locus_tag="AC16CB_14270"
     CDS             5573..6184
                     /locus_tag="AC16CB_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HIR3343683.1"
                     /translation="MIDQRLYEEFRDIGRDIFVAGLTSSHGGNMSVRVGDKIIIKRRG
                     AQFGRLQASDFVETSFQGKDSGIVRASTELIVHRAIYEKTSALAIIHAHPRTAITLSL
                     SRDEIIPIDNEGSYLLRKIPVVSVELASGSKEMADKVSEALRGYKIIMQRGHGCFAIG
                     QTLEEAYHWVSALEEVSDIILEHKLMGEEMIEYRKHSESYKKW"
     gene            6223..6948
                     /locus_tag="AC16CB_14275"
     CDS             6223..6948
                     /locus_tag="AC16CB_14275"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR3343684.1"
                     /translation="MTCMVDMLYPETGWAVVDVLEYLKIEIDFPPEQTCCGQPGFNAG
                     HRSASREVAIQFLRAFKDAEVIVTPSGSCAAMVHHEYPLLFADDRRWRDEALRAAANT
                     WEFTDYLVEGLGIDDLQIRLPRRHTFAFQDACHGMRMLGLGASARTLLKHTGNAIVEE
                     LEEYDVCCGFGGLFSVKMADISGAMLERKINHIQACSAETIVCGDVSCMTHMNGGLAR
                     LQSAKRVRHIADVLADGLPGRLT"
     gene            6945..8345
                     /locus_tag="AC16CB_14280"
     CDS             6945..8345
                     /locus_tag="AC16CB_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HIR3343685.1"
                     /translation="MKVTSNTFIPAAQVALLDDEMRRSVDQATTTAYQKRLNLMFEAG
                     REHGEALRQQAAQAKRHALSRLPALLEQAEARLTANGAHVLWALDSDEVNRAVLEIIA
                     RHSVKAVVKSKSMVSEEVGLNEVLESHGIEVVETDLGEYILQLAGEPPSHIVTPVIHK
                     SLETIRDLFVQKLNMPPTDDARHMVQFTRQILREKFLAADMGITGGNFIIAETGTLCL
                     VTNEGNGRMVTSLPRIHVAIVGIEKVVETLEDFVTLTQVLPRSATGQAMTVYTHLYNS
                     PRKAGETDGPEELYVILVDNGRSEIYGTRYAQVLTCIRCGACMNACPVYRSTGGHAYG
                     WVYPGPVGAVITPLLAGLENAIPLPHASSLCGACKEVCPVDIDLPQMLLDLRDDLYQQ
                     GKVEGLWNWGLKLWAVGNQSPTLFELGGRLARMLTGDRPPKQLPGPLAGWLKYRDFPP
                     FAKKPFRRQWREKRGG"
     gene            8347..>8961
                     /locus_tag="AC16CB_14285"
     CDS             8347..>8961
                     /locus_tag="AC16CB_14285"
                     /inference="COORDINATES: protein motif:HMM:NF014628.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutC/YkgG family protein"
                     /protein_id="HIR3343686.1"
                     /translation="MTTSRDQILSRLRAAQPALGTIETVWEHLPVVPLVDPSPLGLYN
                     RFVEQARLLACIVHLPTSQVEAIQVILNLVGDERRVLCWDFAHIPLPGLEEAFAAHGI
                     LVSEGRDATVRTGITGADAALAATGSLVLLSGAGKPRLASLLPAVHIAVIHQSQILAD
                     LETWIATLRKKGLDAFQGAASAMIISGPSRTADIAMQLTLGMHGP"
BASE COUNT         2158 a         2332 c         2578 g         1893 t
ORIGIN      
        1 ttgatgggac ccttcagcaa gtacaacgcc gacaacatcg acgcagcggt caatccgtaa
       61 gaccttttat aacatgtcag ttgggtatcg ggtattacac ccggtaccca acattcatct
      121 acctcgcgaa taacatggac gactaggaga ggatgagcca tgaaacgggt aggatttcaa
      181 ttcaaagtca ggcaagaccg tcttgcagat tacaaagaac aacacaagca cgtctggcca
      241 gaaatgctgg acgcgctacg cgaagccggt tggcataatt attcattatt catgcgttca
      301 gacggactga tcttcggcta ttttgaaacc gatgagagcc ttgccgttgc ccaggctaaa
      361 atggccgcca aagaggtaaa cacgcgctgg cagaaattca tgtcccattt catcgatgcc
      421 aacgctcgtc ctgatgagac tttccttgag ctggaagagt attttcacct ggattagagg
      481 aggcgaccag tgctgcaaca cattacagaa aatgaaagct ttttagggtg attccggaat
      541 aatcacccca caagtacacg ggagcagtca tgaaccaaca accaaccaca acccagattg
      601 agcaggcgta caaacaggcc agagagcgat atgcggctgt cggtgtgaac acggaagaag
      661 cattggcacg gcttgccgat atcgctatca gcctgcactg ctggcagggt gacgacgtgg
      721 tcggttttga agacccggat cgaggtttga gcggtggtat tatgacaacg ggcaattatc
      781 cgggtaaagc acgcacgccc aatgaactgc gccaggactt agataaagtc tacagcctca
      841 ttcccggcac tcatcgcctc aacttgcatg caatctacct ggaaacagac aataaggtgc
      901 cgcgtaacga gattgaacca aaacatttcg ccaggtgggt ggattgggca aaggagaaca
      961 atcacggaat agatttcaac ccaacttgtt tttcgcaccc gttggctgat gatggcttca
     1021 cgctatccca tcctgatgtt ggtatccgtc aattttggat tgagcattgt attgccagcc
     1081 gggtgattgg agcgcatttc gggcgagaac tgggcacgcc tgccatcacc aatatctgga
     1141 tcccagacgg ctataaagat accccggtgg atcggctggg accacgccgg cgcttgcgtg
     1201 attcgctgga cagaatcctc accgaaccaa cggaccggcg ctacaacctg gattcggtgg
     1261 aaagtaaatt attcggtatc ggctctgaaa gctacgttgt cggctctcac gaattttatt
     1321 tgggctacgc cacagccaac cagacattgc tttgtctcga tgccggtcat tttcacccca
     1381 cagaatccat cgccgacaaa atttcagcgg tgctgctgta cgttccggaa ctgctgctgc
     1441 acatcagccg cggcgtgcgc tgggatagcg accacgtggt caccttgacg gacgagctgc
     1501 aggcgatgat gggtgaaatt gtgcgtggca actatctgga ccgtgtacat attggcctgg
     1561 atttcttcga tgccagcatc aaccgcattg ccgcctgggc tatcggggcg cgcaacgcac
     1621 tgcgggcgct gcttatggcc ttgttggagc ccaccgagca gatgcgtgcg cttgagatgg
     1681 ctggagattt cacagcccgg ctggctctac tcgaagagtt gaaagggatg ccgttgggtg
     1741 caatttggga tagctattgt ttgcagcaaa acgtgccggt tggcatgacc tttctggatg
     1801 aaattcgcgc ctacgaaaat aaggtgttgg cgcaacgagg ttaatattgc ctgccacaac
     1861 agtcctagcc atcgatatag gagccgaatc aggccgagta atggcggtgc atttcgacgg
     1921 caagcaactg gcgtcggaag aacttcatcg ttttcccaac acaacaatta ccgtcaaccg
     1981 cacactccat tgggatttct tacgactgtg gggagatatt caggaaggaa tagagaaggg
     2041 caaagcgctc aatcctgcca gcatcggtgt ggacacctgg ggtgtggatt ttggtctgct
     2101 ggatagccag ggcaacctga ttggcaatcc ggtgcactat cgcgacgggc gcaccgcgca
     2161 catgatggac attgcttttg ccagagttcc ccaagctgac atcttcgccc agaccggtat
     2221 ccagtttatg cccatcaata gcctctacca gttgctgagc ctggtagaaa gccaatcgcc
     2281 gcagttgcaa cttgccgaca ccttcttgac agcgcctgat ttgctcaact actggctgac
     2341 cggcgttaag gtgtgtgaat ttagtaatgc caccaccacc cagctgttca accccaccac
     2401 cggcacctgg gctactggga taatggcgaa gttgggcatc cccagccata ttttccccga
     2461 aatcgtcccg ccgggaaccc ggttggggtt gtatgaaggt attccggtca ttgccccggc
     2521 ctgtcatgat acaggcagtg ccgtggcggc cattcctacc cagagacctg acttcgctta
     2581 catcagcagc ggtacctgga gcctggtagg cctggaagtg aatagcccga tactgacgcc
     2641 ggaagcgctg acagctaatg tcaccaacga aggcggcgtt tacgggactt accggttgct
     2701 gaaaaacgtg atggggctgt ggattttaca gcagtgccgc gccacctggg cggctgaagg
     2761 cgaggtgtac acttatgccg aactggtgaa catggcagca caggcggagc cactagcaac
     2821 cggtttcgac cccaatgatc ctgcatttct ctcccctggc gaccacccgc agctcattcg
     2881 tgacttgtgc agcaaaacaa accagcccgt tccagaaacg ccgggagcgg tggtgcgcag
     2941 cgtcctggaa agtcttgcgc ttgcgtatcg cgaggtgctg gaactggtaa gtgccgttgc
     3001 caaccgccac gtctcagttc tatacatcgt gggcggaggc gcgcaaaacc agctgctcaa
     3061 ccagatgacc gcggatgcca cgcgcctgcc cgttatcgct ggacctatcg aggccaccgt
     3121 catcggcaac gccttggtgc aactcatcac cctaggtaaa attacagaca tcagccaggc
     3181 acgccagata ttagccaata tggctgaact gaagcaatac gagccagtag gaaacgcagg
     3241 ctctaagaca tccgtttggg aagacgcata cgaccgttat cggcgaaatc gtccgcctcg
     3301 ataaccagga gattcacaat ttcggagcga cattatcgtt gagtaagagg gattaggatg
     3361 aaaatcaagc tgttcggcgg gttgcgccag aaggccggga gcaaagagca aacggcatca
     3421 ggcgcgacga ttcgggaagc gctggaaggc atttgtttag ataatgaaac gttacgtgca
     3481 gcgatatttg acggcaatgt gctgcatccc cacgtgcgtg tgatggtgaa cggccgcgat
     3541 tgcgaattgg cgcaaggttt ggatacgccc gtatctgatg acgaccagat cgccgtcttc
     3601 ccaccgattg caggtggtca atgcactaga cgcggaggaa ccgaaaaaat gcgatctgga
     3661 taaaaataga gaatatctct cttttgttat tcttatcttt tcgtgtaact ctcgtaggag
     3721 tttttgacta gcggtagctc atcgtcggcg aataaaaatc taatttgttg ggaggtactt
     3781 atgcaaggtt ggactggaaa aatactggat attaacctca cagacggcag cattgaaaca
     3841 ctacctttag atatggaaat ggcacgcctc tttttagggg ggcgaggcct aggtgcacgg
     3901 ttattgtggg atttggtcgg accagaagtg agtccactct cgcccgaaaa cgtgctcatc
     3961 tttacaacgg gtcccattac ggcaaccggt tcacagacga gtaatcgctt caatgttagc
     4021 accaaaagcc cgctgaccga tactattctg catgccaaca gcggcggctg gtgggggatg
     4081 cagttcaagc gcactggcta cgacgcgctg attgtgcgtg ggaaatcgaa aacaccggtc
     4141 atgatcgaga tcaaaccgga aggcgtggaa ttgaaagaag caggtcacct gtggggcaag
     4201 ggtgtgtttg aaaccaccga ggcattggga caaagccgca accagcgcaa tgtgttgtgc
     4261 attggccccg ccggtgagaa ccaagtgcgc attgctgcca ttatgaatga caaagaacgc
     4321 gccctggcgc gcggtggccc tggggctgta atggggagca agaatctgaa ggcgattgtg
     4381 gttgaaggga aggagaagaa tcagccggcc gatcaagaac tgttcaaatt catgctgtac
     4441 gaaaccggta aactgttgaa agcgagcccg ttgacatcac aggggctgcc ggaattcggc
     4501 acggctgttg tcatgaacgt tgtcaatgaa atcggtgcac tgcccacccg taatttccag
     4561 caatcccagt ttgaaggtgc ggacaaaatc agcggcgagg caattaccga cagcatcctg
     4621 gtgaaaaatc aggcatgttg ggcgtgtccg attacctgta cgcgcatcac aaaaacggac
     4681 agcggcaaag agggagaggg tccagaattt gaatccgcat gggcgtttgg ggcgcagtgc
     4741 ggcatcgacg acctggatac gatcaccgaa gccaactacc tgtgcaacga catggggctg
     4801 gacacgattt cgatgggcaa tacgattggc tgcgcgatgg aactggccga aaaagggctg
     4861 attgcaaacg agttcggctt tggccaggcg ggcaagctgc tggacctgat tagcgacacg
     4921 gcgtatcggc gtggactcgg ggcggaaatg gcagaaggca gctatcgcat ggcagaaaaa
     4981 cacggcgcgc cagagctgtc tatgagcgtc aagaagctgg aaatgcccgc ctatgatcca
     5041 cgtgggatgc aggggcaagg gttagttttt gctaccggca accgtggtgc ctgccatgaa
     5101 accggtaata tgttgggacc ggaggtgctg gcattgccgc gtctcattga ccggtttgct
     5161 acgcagggca aagcgggtat cgtgtccgtc catcaaaact cggcagcggt gattgacagc
     5221 ctggtgtact gtaagtttac aaacatggcg gtggcagagg agttcttcgc tcggacgtta
     5281 tcggctgtta ccggcgaaca tttcaccgct gatgacttga tgatggttgg tgaacgcgtc
     5341 tggacgctgg agcgtctcta caacttgcgc gagggcttta cgaagaaaga tgataccctg
     5401 cccgaccgaa tgctcagcga acccgtccct gatggaccga gcaaaggctt taccgtcaag
     5461 ctggctccta tgctggaaga atattatgca ttccgaggct gggaccaaaa cggcgtgcct
     5521 aagccagaga agcttaagga actatcttta gagttcgcaa ttgaggaagc acatgattga
     5581 tcaacgatta tatgaagaat tccgagatat tggccgagat atttttgtgg caggcttaac
     5641 cagctcacat ggcggcaata tgagcgtgcg ggtgggggac aagataatta tcaaacgacg
     5701 cggggcgcag ttcgggcggt tgcaggcctc ggattttgtg gaaaccagct ttcagggcaa
     5761 ggacagtggc attgtgcgcg ccagcaccga attaatcgtc caccgcgcca tctacgaaaa
     5821 aacatcggcg ctggcaatta tccatgcgca tccacgcacc gccatcactc tcagcctaag
     5881 ccgggatgag atcattccca tagacaacga aggctcctac ttgctgcgca agattccggt
     5941 ggtgtcggta gaactcgcct ccggctccaa agagatggcg gataaagtct ctgaagcgct
     6001 acgcggctac aaaatcatca tgcagcgagg gcatggttgc ttcgcgattg ggcaaacgtt
     6061 ggaagaagca taccattggg tctccgcatt agaagaagtg agcgatatca ttctggagca
     6121 caagctgatg ggcgaggaga tgattgaata tcgcaagcac agtgaatctt acaaaaaatg
     6181 gtaaagcgcg attctcccaa gggtaagcgg gcgtctttat ttatgacatg tatggtggat
     6241 atgctctatc cagaaacggg ctgggctgtg gtggacgtct tggagtactt gaagattgag
     6301 atagactttc cacctgagca gacttgctgc gggcagccgg ggttcaacgc cggacacagg
     6361 tctgcctccc gtgaggtggc gatccaattc ctgagggcgt tcaaagacgc agaagtgatc
     6421 gtcactccat ctggctcgtg tgcggcaatg gtgcaccacg agtatcccct cctttttgcg
     6481 gacgatcgcc gctggcgcga cgaagcgctg cgggcagctg cgaatacctg ggaattcaca
     6541 gattacctgg ttgaaggact tgggatcgac gaccttcaaa tacgattgcc ccggcgacat
     6601 actttcgcgt tccaggatgc ctgccacggt atgcgcatgc tcggtctggg agcttcggct
     6661 cgcaccttgt taaaacatac gggcaacgca attgtggaag agctggagga atacgatgtg
     6721 tgctgcggct ttggagggct attcagcgtc aaaatggcag acatcagcgg ggcgatgctc
     6781 gagcggaaaa tcaaccacat acaggcttgc tctgcagaga cgatcgtgtg cggcgatgta
     6841 agttgcatga cccatatgaa tggcgggcta gcgcgcctac agtccgccaa acgcgtgcgg
     6901 cacatcgccg atgttctggc tgatgggctg ccaggaagac tcacatgaaa gtcacttcca
     6961 acacctttat cccggctgca caggtggctt tgctggatga cgaaatgcgc cgctcggtcg
     7021 accaggcaac gactacagcc taccagaagc gcttgaattt gatgttcgag gccggtcgcg
     7081 aacatgggga agccctgcgc cagcaagccg cccaggcgaa acgacatgcc ttgagtcgcc
     7141 tgccagcatt attggagcag gctgaagcca ggttgaccgc caatggcgct cacgtcttgt
     7201 gggcgctgga tagcgacgaa gtgaaccgcg ctgtgctgga aattatcgcc cgtcattcag
     7261 tgaaagccgt ggtgaagagc aagagcatgg tgagcgaaga ggtcgggctg aacgaggtgc
     7321 tcgaatccca tggtatcgag gttgtcgaaa ccgacctggg ggagtacatc ctccagctcg
     7381 caggcgagcc ccccagccac atcgttacac cagtcattca caaatcttta gagaccatcc
     7441 gcgacctgtt tgtgcagaag ttgaatatgc cccccacgga cgacgctcgc catatggtgc
     7501 aattcacacg ccagatcttg cgtgaaaagt ttctcgctgc ggatatgggc attaccggcg
     7561 gcaacttcat tatcgccgaa acgggaacct tgtgcctggt gactaacgaa ggcaatggtc
     7621 gcatggtcac cagtttgcct cgcattcacg tggctatcgt tgggatcgaa aaagtcgtcg
     7681 agacgctcga agattttgtc acactaaccc aggtgctgcc gcgcagcgca accgggcagg
     7741 ccatgacggt atacactcat ctatacaaca gcccccggaa agcaggggaa acagatgggc
     7801 cggaggagct gtacgtcata ctcgtcgata acgggcgcag tgagatctat gggacgcgat
     7861 atgcacaggt actcacctgt atccgctgtg gagcgtgcat gaacgcctgc ccggtttacc
     7921 gatcgaccgg tggccacgcc tacggctggg tgtatcccgg cccggtaggc gcggtgatca
     7981 cccctttgct agccggattg gaaaacgcta taccgctgcc tcacgccagc agcctgtgtg
     8041 gggcatgtaa agaggtctgc ccggtggata tcgacctgcc gcagatgctg ctcgacctgc
     8101 gagatgacct gtaccaacag ggcaaagttg aaggactctg gaattggggg ctgaaattgt
     8161 gggcggtcgg gaatcaatct cccaccttat tcgagttggg tggcaggctc gctcgcatgc
     8221 ttacaggcga tagaccaccg aaacagttgc cgggacctct ggcagggtgg ttgaaataca
     8281 gggatttccc gccttttgca aagaaacctt tccgccgcca gtggcgggaa aagcgaggcg
     8341 gatgagatga ccaccagccg ggaccagatt ctaagtcgcc tgcgcgccgc ccagccggcg
     8401 ctaggtacca tcgagaccgt atgggagcat ctgcccgtgg tccccttggt tgacccgtcc
     8461 cccctgggac tgtacaaccg ttttgtggaa caggcgaggt tgctcgcctg tatcgtccat
     8521 ctacccacct cccaggtcga agccatacag gtcatactga atttggtggg cgacgagcgc
     8581 cgcgttttat gttgggattt cgcgcacatc cctctacccg gattagaaga ggcgtttgca
     8641 gcacatggca tcctggtctc cgaggggcga gacgcaaccg tacgtacagg aatcactggc
     8701 gccgacgctg ccctggctgc cacgggcagt ctcgtcctgc tgagcggcgc aggcaagcct
     8761 cgcctcgcct ctctgctgcc agccgtgcac attgccgtta tccaccagag ccagatcctg
     8821 gctgacctgg aaacctggat agccacccta cgcaaaaagg gattggatgc ctttcagggg
     8881 gcagccagcg caatgatcat cagcggcccc agtcgcacag cagacattgc catgcagtta
     8941 acgctgggta tgcacgggcc g
//
LOCUS       DBNGSX010000482        10806 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_463710, whole genome shotgun sequence.
ACCESSION   DBNGSX010000482 DBNGSX010000000
VERSION     DBNGSX010000482.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10806)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10806)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10806
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_463710"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="AC16CB_14290"
     CDS             complement(<1..1238)
                     /locus_tag="AC16CB_14290"
                     /inference="COORDINATES: protein motif:HMM:NF016736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HIR3343687.1"
                     /translation="MKTQILRLDPHDDVISTRDKMNWGQTGRILLLWPEHGRVLRRRV
                     DLVLLHRHSNSLGAQLALVTNDPLVRFHAHYLAIPVFKNLRQAQSAHWRVNRQHRVRQ
                     ATKFVARPLLTSEALEELRQGAHPPTPAWLSKPFARLGFFGLGVIALLALAAVLLPGA
                     EVSLTPETQSQQLTITVRADPDQQDINYSGIVPARPVSVIVEGRDSIEASGVDQVADK
                     PSTGNVVFTNLSDQPVKIPVGLVVRSVSDPSVRFATIEAVEVAAGPGITTTLAVRAVT
                     PGKGSNLPAGSLNAIEGNFGLILAATNPAPTAGGTERTLPVPTIQDRKRLYEKMEAAL
                     RISALNEVQSQFAPDDLLFTPTLTITKVLDETYTPSSDQPSEQLQLNLRLEFQILSVF
                     RSDLENLAVVLLDTNLPNGFV"
     gene            complement(1249..2802)
                     /locus_tag="AC16CB_14295"
     CDS             complement(1249..2802)
                     /locus_tag="AC16CB_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR3343688.1"
                     /translation="MDKKQAYQFPILHPLLIAAYPVVALLAHNIEEIKIMVALRAMFI
                     SLMIGVLIYLALRSLFNDTTKAAVLTTLALALFYSYGHVYNSLERSALFGIILGRHRI
                     LAPIWMGILLASMIWVYRTKNPFSGLNRLLNLVTVVALALPLAQIGLFGLRSMSATTE
                     APGQINIAGLHLPSDRPAPDVYYIILDAYARDDTLIEEFELDNTPFLSQLEAMGFYVA
                     RCSQSNYVQTQLSLSSSLNMDYLQSLDPGYSPGRKTRVGIEELIRHSAVRQAFKNLGY
                     TTIAFETGFKGTQWEDADIYLSPSNDLFHKLQIAGGPNGFEVLLLRTSAGLLLADGAV
                     VLPKIFQPDFDNPRKIHRDLILFDLEQLHVLPTIPGPKFVFAHLVTPHPPYVFGPNGE
                     FTDFDKEYKPGYRDQVIYLNKRLLELIPTMIANSPVPPIIILQGDHGSIGSPPNKRTT
                     ILNAYYLPDGGNKSLYESISPVNTFRIIFNSYFEGQYELLEDVSYYSVYTDPFDFTVI
                     PNKRADCPS"
     gene            complement(2846..4354)
                     /locus_tag="AC16CB_14300"
     CDS             complement(2846..4354)
                     /locus_tag="AC16CB_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343689.1"
                     /translation="MRKIPILHPFLFTAYPVVAFLAHNIEQTEINSALRPLLVGLASA
                     AILYILLKAVYRDNHRAGLLTSFLLILFFTYGHVYNAIEGFSILGMNIGRHRLLAPLW
                     MALAGFSFWRGFQSRRDLQPLSNRANIIALIALTMPLGQISAYQISLRSVRIGSANSK
                     VFSSAQIDPNRPLPDIYYLILDSYSRDDMLKKYYDLDNTPFIDQIEQMGFYVARCAQS
                     NYGQSELSMASSLNYDYLDNLGRQFNPENRRRTGLADLIQHSAVRSLLEDLGYTVVAT
                     DTGYDATRLSDADIYLAPLVSAEINDFEETFLRTTAARLFIEGAAILKLPPDWEKRDQ
                     AHRERILFQLEQLRQIPAIPGPKFVFAHIITPHWPYIFGPNGEPVHEHPDSKTGYRDQ
                     VIFINRQIVPILENILATSEIPPIIVIQGDHGAVLVDPKRRMSILNAYYLPDGGDALL
                     YEGISPVNTFRLIFNHYFGLQVPLLDDVSYYSSYEEPYNYQLVENQRKGCND"
     gene            complement(4354..5748)
                     /locus_tag="AC16CB_14305"
     CDS             complement(4354..5748)
                     /locus_tag="AC16CB_14305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3343690.1"
                     /translation="MTSANFQGLTRSVEASFRDPSGHLFTLDGTLFRQVNRFYQEHYT
                     HLMDSGLYQSLVKDGLLIPHDEVSVTPPDPSLAWKVIQPETIPFISYPYEWSFSQLKD
                     AAQVTLEIQKRALEHGMVLKDSSAYNIQYHQGKPVLIDTLSFEIYTEGQPWIAYRQFC
                     QHFLAPLSLMSHRDIRMGQLLRIYIDGVPLDLASRLLPWKTRFNWPLQMHIHLHAASQ
                     KRYASSSLPSERKMGKTAMLGWIDSLESAIRGLSWSPEGTAWSEYYTDTNYTSSGLEH
                     KKALVREYLDFFHPKSIWDLGANTGLFSRIASQMGIQTTAFDIDPGAVELNYRECRKE
                     SETNLLPLLLDLTNPSPGIGWENRERQAFAERGPADAVMALALIHHLAIANNVPLQRL
                     ASFLHGLSQWLLIEFIPKGDSQAQRLLASREDIFTEYTQANFEQVFASRYTIHRVEAI
                     QDSQRSLYLMEARK"
     gene            complement(5745..5963)
                     /locus_tag="AC16CB_14310"
     CDS             complement(5745..5963)
                     /locus_tag="AC16CB_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343691.1"
                     /translation="MSIMSILSPLVEILTAPAKANPAYLDPGSGSFILQLLLAALVGS
                     AFIIKSYWKKITDFFRKLFSRADQRKEE"
     gene            complement(5976..7121)
                     /gene="rffA"
                     /locus_tag="AC16CB_14315"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(5976..7121)
                     /gene="rffA"
                     /locus_tag="AC16CB_14315"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose:2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HIR3343692.1"
                     /translation="MDKEIFIDFNRPALVGNELAYITEAVSRLHLSGDGYYTHLCQDI
                     LAEELGARKVLLTTSCTHALEMAALLLDVQPGDQVIVPSFTFVSTINAFVLRGAQPVF
                     IDIRPDTLNLDENQLEGLVNERTKAILPVHYAGVGCEMDDILKIAEKYNIPVVEDNAH
                     GLFGKYKEKLLGTFGCMATLSFHETKNISCGEGGALLINELEYIERAEIIREKGTNRS
                     RFFRGQVDKYTWVDIGSSYLPSDILAAFLYAQLEKRQVIQARRKAIWDRYYEGLASWA
                     EQFPVRLPIVPQHCEQPYHMFYLLLPSLEIRQSLISYLKHTGIQSVFHYQPLHLSEMG
                     RRLGGIPGDCPVTEEISDQLLRLPFHAHLEEADQMRVIQAITSYQQK"
     gene            complement(7125..7820)
                     /locus_tag="AC16CB_14320"
     CDS             complement(7125..7820)
                     /locus_tag="AC16CB_14320"
                     /inference="COORDINATES: protein motif:HMM:NF020463.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HIR3343693.1"
                     /translation="MKCVLLQPSYIPWRGYFHQIHKADIFVFYDDVQFDKHGWRNRNR
                     IKTHQGTIWLTIPVQSHTAPLHHTPINEVIIDNHQPWNRKHWMTLSQSYGKAPFFSRY
                     ADLLEAYLRQPQELLAEFTIELTIALSKELGIMDTQFVRSSSLTVTGTKTERLIQIVN
                     CLGADHYITGPAAKSYIDQSQFDLAGIRLEYMEYNYPDYEQLYPPFDPQVSILDLMFM
                     TGPNALDYIVGKG"
     gene            complement(7817..8782)
                     /locus_tag="AC16CB_14325"
     CDS             complement(7817..8782)
                     /locus_tag="AC16CB_14325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3343694.1"
                     /translation="MKNLSIVIPVYNSESTLGDLVERLAKVLPSLAPVYEVILVNDGS
                     RDRSWEIIDQLIQTYSWASGINLMRNYGQHNALLCGVRQARYEIIVTMDDDLQHPPEE
                     IHKLLDVLSQGNDVVYGIPHKQPQNWWRNIFSMIIRRLLAYVMGVKNIRDFASFRAFR
                     AYLRGAFADYQNPNVVLDVLLAWGTTRFANAVVTEMPRTVGRSNYNFFKLVQYTMIVL
                     TGFSTVPLRITSMLGFIFTFVGIAIFLYVLAIYFLVGSIPGFPFLASIISIFSGTQLF
                     ALGIIGEYLARIFDRSMDRPAYVVRESIRHGEGYITGIEDKVEAA"
     gene            complement(8860..>10806)
                     /locus_tag="AC16CB_14330"
     CDS             complement(8860..>10806)
                     /locus_tag="AC16CB_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343695.1"
                     /translation="FYWDDWPSIYYLHVLGPRGFIDAFAVDRPTLGWFFMLTSSIVGE
                     SMLAWQIFGLLSRWLSCLALWWMLRVLWPNHDRQVTWTALLFAAYPGFLQQYIAVTYS
                     HTWLVLATFLLSMGTMIWAIRKPMYFWPLISLSCILSAWCMFTDEYFFGLELLRPIIL
                     WLVVQPKGNGLTKRLTSVTIRWLPFVAVIAAFLIWRLFIHVSPRGQVRIFDKISESPI
                     IAIREIVTTVAQDIVESSLIAWGQVLNIPRQTSYGMGPTVLYLFVTALSIALAMIYLS
                     RFDLQTSGGSPTGETRHWSRQAIFLGLAGLLFAGIPFWVTNLPIELRFPWDRFTLAFN
                     LGASLLLAGVLELLPKRRILNVLILATLLGLASGMHEQLANLYRREWNSQKALFWQLV
                     WRAPGIQPGTMLLSAELPFIYYSDNSLTAPLNYTYNPAQPSSELSYMFYNIESRLGSK
                     LEGFEKGQDVNENYRALYFSGTTSQALAIYYSPPGCAKVIDPATDAKTPQKPLYFSRV
                     LSLSDLGFIQATATPPAMPPKAYFGDEPEPNWCFYFEKADLARQQSDWAEVVRLGEQA
                     FQLNHRLYEVNATELLPYIEGYARTGRWEKARELTNEAHQLTFRMNSILCETWKRIQG
                     QDGLPLEGQNTVNRVKQRLKCATN"
BASE COUNT         2726 a         2513 c         2893 g         2674 t
ORIGIN      
        1 acaaaaccat ttggcaagtt tgtatccaag agcacgaccg ccaagttttc caaatcggaa
       61 cgaaacacgc tgagtatctg gaattccagg cgcagattga gttgaagctg ttcagagggc
      121 tggtcgctgc ttggtgtata agtctcgtcc agcactttgg taatggttag tgttggtgta
      181 aagagcaggt cgtcgggagc aaattgcgat tggacttcat tcaaggcaga tattcttaga
      241 gcagcctcca ttttttcgta tagccgcttc ctgtcttgga tggttggcac gggaagagtg
      301 cgctcggtgc caccagcggt tggagcagga tttgtcgcag ctaggatcaa cccgaaattc
      361 ccttcgatgg cgttcaggct cccagctggc aagttacttc ctttgccagg ggtgacagct
      421 cgcactgcca gcgtggttgt aatacccgga ccggcggcga cctcgacggc ttcaatggta
      481 gcaaaacgta ctgatggatc agacactgag cgcactacca atccaacagg gattttcacg
      541 ggctggtcgc tcaggtttgt gaaaactaca ttacccgttg atggtttatc ggcaacttga
      601 tcaaccccac tggcttcgat gctatcgcgc ccctcaacga tcactgaaac cggtcgggca
      661 ggtactatgc ctgaatagtt gatatcctgc tggtctgggt cggcgcgcac ggtaatggtg
      721 agttgctggc tctgggtctc gggagtgagg gacacctccg ccccgggtag gagtacagct
      781 gccaaagcca gcaaggcgat tactcccaaa ccgaagaatc ccaatcttgc gaatggtttc
      841 gatagccagg caggagtggg gggatgtgct ccctggcgca gttcctccaa cgcttcgctc
      901 gtcagaagcg ggcgagcgac gaattttgtc gcttggcgca cccggtgctg tctatttacc
      961 ctccaatgag cgctctgcgc ctggcgcaaa ttcttgaata caggtatagc aaggtaatgg
     1021 gcgtgaaagc gtaccagcgg gtcattggtg actagcgcca gttgtgctcc cagggaattg
     1081 ctgtgccgat gcaggagcac caggtctaca cggcggcgca aaacgcgacc gtgttcaggc
     1141 cataacaaca atatacgccc ggtctgtccc cagttcattt tgtctcgggt agatattaca
     1201 tcatcatgtg ggtccaagcg cagaatttgt gttttcattc aaccgctctc aagatggaca
     1261 atccgcacgc ttgtttggga tgacggtaaa atcaaatggg tcagtgtaga cagaatagta
     1321 gctcacatct tccagcagtt cgtattgacc ttcaaaatat gaattgaata ttatacgaaa
     1381 agtgtttact ggggagatcg attcgtagag ggatttattt cctccgtccg gaaggtaata
     1441 ggcattaaga atggttgtac gtttattggg agggctgcca attgagccgt gatctccctg
     1501 aaggatgatg attggtggga cgggtgagtt ggcgatcata gttgggatga gctcaagtaa
     1561 gcgtttgttc aagtagatca cctgatcgcg gtatccgggt ttgtactctt tatcaaaatc
     1621 ggtaaactct ccatttggac cgaatacata gggagggtgc ggggtcacca ggtgtgcgaa
     1681 gacgaacttg ggacccggga tagttggtag gacatgcaat tgttcgagat caaagaggat
     1741 caaatcccgg tggatttttc gaggattgtc aaaatctggc tggaatatct ttggcaagac
     1801 gacagcccca tcagccagaa gcaggcctgc cgacgtcctt agcagcagca cctcaaatcc
     1861 attcggcccg cctgcgattt gcagcttgtg gaataagtca ttggagggag ataggtaaat
     1921 gtcggcatct tcccattgtg tgcccttaaa tccggtctca aacgcgatgg ttgtatatcc
     1981 tagattttta aaagcctgcc gcactgcgct gtgtcggatt aactcttcaa tgcccacccg
     2041 tgtcttacgt ccaggagaat agcctggatc taaagattgc aggtagtcca tatttaggga
     2101 tgacgagaga gatagctgag tttgtacata attgctctgg ctgcagcggg cgacgtagaa
     2161 acccattgcc tccaattggc tcaggaatgg ggtgttatcc agttcgaact cttctatcaa
     2221 ggtgtcgtcg cgagcgtagg catccaggat gatgtagtag acatcgggcg cgggtcgatc
     2281 ggaggggaga tgtaatcctg cgatattgat ctgacctggc gcttccgtag ttgcggacat
     2341 gcttcttaac ccaaataatc caatttgggc caggggtaga gccaaagcga ccacagtaac
     2401 caagttgagc agacgattca aaccagagaa tggatttttt gtccggtaaa cccagatcat
     2461 ggatgccagc aatattccca tccagatggg tgccaaaatg cgatgtcttc ctagaatgat
     2521 accaaaaaga gctgatcgtt ccagtgagtt gtaaacgtgc ccataagaat agaaaagagc
     2581 taatgccaaa gttgtcaata cagcagcttt ggttgtatcg ttgaacagac tcctcagggc
     2641 gagatatatt aggacgccga tcatcaggga aatgaacatg gcgcgcaagg cgaccatgat
     2701 cttgatctcc tcgatgttat gcgcgagcag ggcgaccacc gggtaagcgg caatcagcag
     2761 ggggtggagt ataggaaatt gatacgcttg tttcttgtcc aaaacacgat cctcccgccc
     2821 catgtgcggg ttgcggaggg ggtgatcaat cattacaccc tttgcgttga ttttctacca
     2881 actggtaatt atacggctct tcgtagcttg aataataact gacatcatct aagagcggta
     2941 cctggaggcc gaaataatga ttgaatatca ggcggaaggt gttgacaggg ctgataccct
     3001 cataaagcaa agcatcccct ccatcaggta gataatatgc atttaatatg ctcatcctgc
     3061 gcttgggatc caccagcact gccccatgat ccccctggat gacgatgatt ggaggtatct
     3121 cagatgtggc aaggatgttc tctaagatgg gcacaatttg ccggttgatg aagatcacct
     3181 ggtcgcggta cccggttttc gagtcgggat gttcatggac tggctcacca tttgggccga
     3241 agatgtaagg ccagtgaggg gtgatgatgt gggcaaagac gaacttagga cctggtatag
     3301 caggaatttg tctcaattgc tccagttgga atagtatccg ctcgcggtgc gcttgatcgc
     3361 gtttctccca atctgggggt agtttaagaa ttgctgcgcc ttcgatgaac aggcgggcag
     3421 cagtggtccg caggaatgtt tcttcgaaat cgttgatttc tgctgataca agtggggcca
     3481 ggtatatatc cgcgtcactc aggcgggtag cgtcatagcc cgtgtctgta gcgacgacgg
     3541 tgtagcctaa gtcttccaat aacgaccgca ccgcgctatg ctggattaag tctgccaagc
     3601 cagtccgtcg ccggttttct gggttgaact gccggcccaa attatccaga taatcgtagt
     3661 ttagtgaaga agccatagat agctcagatt ggccgtaatt gctctgggca cagcgtgcga
     3721 cgtaaaaccc catttgctca atttgatcga taaaaggggt attatccaag tcatagtatt
     3781 tcttgagcat atcatcgcgc gaataactgt ccagaattag ataataaata tctggaagag
     3841 ggcggtttgg gtcgatttgg gcactggaaa acacttttga atttgctgag ccaattctga
     3901 ccgatcgaag gctgatttgg tatgcactga tctgtcccag cggcatggtg agcgcaatta
     3961 aggcgatgat attggctcga ttacttaatg gctgcagatc acgccttgat tgaaatcccc
     4021 gccaaaagga aaaaccagcc aatgccatcc agagcggcgc cagcaagcga tggcgaccga
     4081 tgttcatccc taggattgag aaaccttcaa tagcgttata gacatgccca taggtgaaga
     4141 ataagatcag caaaaatgag gtgagcaagc ctgctcgatg attatccctg tatacggctt
     4201 tcaacaagat ataaaggatt gcggcacttg ccaaccctac cagcaacggg cgcagcgctg
     4261 agttaatctc ggtttgctca atattatgcg ccaggaaagc cacaaccggg taggcggtga
     4321 atagaaaggg gtgcaaaatg gggattttgc gcattacttg cgtgcctcca tcaggtagag
     4381 ggagcgttgc gaatcttgga tggcctcaac ccgatggatg gtgtaacgcg atgcgaagac
     4441 ctgctcaaaa ttagcctgcg tgtactcggt aaagatatcc tctcgagagg caagcaagcg
     4501 ctgtgcctgg gaatcaccct taggaataaa ttcaatcaac aaccattggc ttaatccgtg
     4561 caggaatgaa gccagacgct gcaaagggac attattggcg attgctagat ggtggatcaa
     4621 agccagagcc attaccgcgt cagccggacc gcgttcagcg aaggcctgcc gttctcggtt
     4681 ttcccagcca atccctggac tcgggttggt caagtcaagc agcagcggca gcaggtttgt
     4741 ttccgactct ttgcggcatt cccggtaatt caactccact gcccctgggt cgatgtcgaa
     4801 agcagttgtc tggattccca tttggctggc aatacggcta aatagcccgg tattggctcc
     4861 caagtcccaa atgcttttcg ggtggaaaaa atccagatat tcgcgcacaa gcgctttttt
     4921 gtgctccagc ccgctggagg tatagttggt atcggtgtag tactcgctcc aagcagtacc
     4981 ctctggcgac caactcaacc cgcggatagc cgattccaag ctgtcaatcc agcccagcat
     5041 ggcagttttt cccatcttcc gctcgctagg caagctgctg gaggcatagc gcttctgcga
     5101 tgcagcgtgc agatggatgt gcatttgcaa tggccagtta aagcgcgtct tccagggaag
     5161 caaccggcta gccaggtcta gcggtactcc atcgatatat atccgtagta attgacccat
     5221 acgaatatca cggtgggaca tcaggctaag cggtgccagg aagtgctgac aaaattgacg
     5281 gtaggcgatc caaggttgcc cctcagtgta gatttcgaag gagagtgtat cgatcaaaac
     5341 cggcttaccc tgatgatatt gaatattgta tgcggagctg tctttgagca ccatgccatg
     5401 ctcaagagcg cgtttctgaa tctcaagggt aacctgagct gcgtctttga gctggctgaa
     5461 actccactca taggggtaag aaatgaacgg aattgtctca ggctggataa ctttccaggc
     5521 taagcttggg tctggcgggg taacactcac ctcatcgtgg gggatgagca atccgtcttt
     5581 caccaggctt tgataaagac cagaatccat caggtgggta tagtgctcct gatagaaacg
     5641 gttcacttgg cgaaagaggg tgccgtccaa agtgaacagg tgcccgctcg gatcacgaaa
     5701 agaggcttca accgagcggg taaggccttg gaaattggct gaggtcattc ttcttttctt
     5761 tgatctgctc gcgagaataa cttccggaag aagtcggtga tctttttcca atacgatttg
     5821 atgatgaagg cgcttccaac cagcgctgca agcagcagtt gcaagataaa gctgcctgag
     5881 ccaggatcca gatatgctgg atttgctttg gcgggcgcag taagtatttc taccaagggt
     5941 gaaaggattg acatgattga catattttct ttgactcact tttgttgata ggatgtaatc
     6001 gcctggatta ctctcatctg gtctgcttct tccaggtgag cgtgaaatgg caggcgcaat
     6061 aattggtcgc tgatttcctc ggtaacaggg caatcacctg gaatccctcc gaggcgtcta
     6121 cccatttcgg ataggtgaag gggttgataa tgaaatacgc tttggatgcc tgtatgtttt
     6181 aagtatgaga tcagtgactg gcgaatttct agggagggta gcagcaagta aaacatgtga
     6241 taaggctgct cgcagtgttg cgggacgatt ggcaatcgca cagggaattg ttctgcccag
     6301 cttgccagcc cttcgtaata cctgtcccag atggccttgc ggcgcgcctg tataacctga
     6361 cgcttttcta attgtgcgta aagaaaagca gccaggatat cggaaggcaa atagctcgag
     6421 ccaatatcga cccaggtgta tttatctacc tgcccgcgga agaaacggct gcgattagtg
     6481 cccttttcgc ggatgatctc agcccgctca atgtattcaa gctcgtttat gagcagggcg
     6541 cctccttctc cgcaggagat attttttgtc tcgtggaagc tcaacgtggc catgcatcca
     6601 aaggtcccca ggagtttttc cttatacttt ccgaacaaac cgtgggcgtt atcttcgacc
     6661 accgggatgt tgtatttttc ggcgattttc aaaatatcgt ccatctcgca gcccacaccc
     6721 gcataatgca cagggaggat cgctttggtg cgttcattga ccagcccctc gagctggttt
     6781 tcatccaggt tgagcgtatc tggacgaata tcgataaaca ctggctgagc gccgcgcagt
     6841 acgaaggcat ttatcgtgga cacaaaggtg aaggagggta cgattacttg atcccctggt
     6901 tggacatcta acaacagggc agccatttcc agggcgtgtg tgcaggaggt agtcagcaaa
     6961 acttttcgtg cacctaattc ttccgcaagg atatcttggc agaggtgtgt gtagtatcca
     7021 tcgccggaga ggtgcagcct gctaaccgct tcagtgatat acgccagttc gttgccgacc
     7081 agagctgggc gattgaaatc gatgaaaatc tctttatcca atgattatcc tttgccaaca
     7141 atgtaatcta acgcattcgg gccggtcata aacatcaagt ccaaaataga tacctgtggg
     7201 tcaaaagggg ggtaaagctg ttcgtaatcg gggtaattgt actccatata ttctaatcta
     7261 atccctgcca agtcaaattg ggattggtca atatagcttt ttgcagccgg acctgttata
     7321 tagtggtctg ctcccaggca gttcacaatt tggatgagcc gctcagtttt ggtgccggtc
     7381 actgtaagcg aagatgaacg aacaaactga gtatccataa ttcccagctc tttagatagg
     7441 gcaatggtta gctcgatggt gaattcagcc agcaattcct ggggttggcg gagatatgcc
     7501 tcaagtagat cggcataacg cgagaaaaaa ggtgctttcc cgtaagattg tgataaggtc
     7561 atccaatgct tgcgattcca gggttggtga ttatcaataa ttacttcatt gatcggtgta
     7621 tgatggagcg gagcggtatg gctctggacg gggatcgtta accatatagt tccttggtga
     7681 gtcttgatcc ggttgcgatt acgccagcca tgtttgtcga attggacatc atcgtaaaat
     7741 acaaagatat cggctttatg tatctggtga aaatagccgc gccaggggat ataagatggc
     7801 tgcagtagga cacacttcat gcagcctcga ccttgtcctc gattcccgtg atgtaacctt
     7861 ccccatggcg tatcgattct ctgaccacat aagccgggcg atccatgctg cggtcgaaga
     7921 tgcgtgccag gtattcgccg ataatcccca gggcgaataa ctgtgtgcca ctaaatatcg
     7981 atataatgga cgccaggaat gggaatcctg gaatactgcc aaccaagaaa tagattgcca
     8041 ggacgtacag gaagatagcg attccgacaa aagtaaaaat gaaacctaac atgctggtga
     8101 tgcgtagagg tacggtgcta aatccggtta gcacgatcat cgtatattgc actaatttga
     8161 aaaagttgta atttgagcgt ccaactgtgc gtggcatttc tgtgactacg gcattggcga
     8221 agcgggtcgt gccccatgca agaagcacgt ccagaacgac atttggattt tgatagtcag
     8281 caaaagctcc gcgcaaataa gcacggaaag cccggaaact ggcgaaatca cgtatattct
     8341 tgacgcccat cacgtaagcc aacaagcggc ggatgatcat cgagaagatg ttccgccacc
     8401 aattttgcgg ttgcttgtgg ggtatgccgt atacaacgtc gttaccctgg gataatacgt
     8461 cgagcaattt gtggatctcc tcaggggggt gctgcaggtc atcatccatg gtgacgatga
     8521 tttcataacg agcttggcgc accccgcaca agagagcgtt gtgttgtcca taattacgca
     8581 tcaagttgat accagacgcc catgaatagg tttgtatcaa ctgatcgatg atctcccagg
     8641 agcgatcgcg gcttccatca ttgacaagga tgacctcgta cacgggtgcc agcgatggta
     8701 gaactttagc taggcgctct accaagtctc caagggtgct ctcgctgtta taaaccggga
     8761 taacgattga caaattcttc ataggcgatt tcaggcaaca taatcactga ccgaggctac
     8821 gcgactaggt actccttaaa ttcaagtggg agaatttgat caattggtgg cgcatttcag
     8881 cctctgcttt acacggttaa cagtattctg cccctcgagc ggcagcccat cttgtccttg
     8941 aatccttttc caggtctcgc ataggatgct attcatgcgg aatgttaact ggtgcgcttc
     9001 attagttaat tctcttgcct tttcccatct cccggtccgg gcgtagcctt cgatgtaagg
     9061 aagcagttcg gttgcattga cctcatacag gcggtggttc aattggaatg cttgctcacc
     9121 caggcgtacc acctcagccc aatcactttg ttggcgagct agatctgctt tctcaaaata
     9181 aaaacaccag ttcggctccg gttcatctcc aaagtacgct tttgggggca tcgcgggtgg
     9241 ggtagctgtg gcttggataa atccaagatc agataacgac aataccctcg aaaaatatag
     9301 cggtttttgc ggagttttgg catccgtagc tggatcgata accttggcac aaccgggtgg
     9361 gctatagtag attgccaaag cttgggatgt tgttccgcta aaatatagcg cccggtaatt
     9421 ttcgtttaca tcttgacctt tttcgaagcc ttccagcttg ctccccaagc gagattcgat
     9481 attataaaac atgtaggata gctctgacga tggttgcgca ggattatagg tgtaattcaa
     9541 gggtgcagtt aatgaattgt cgctgtaata aataaatggc aattcggcgg agagtagcat
     9601 cgttccgggc tggataccgg gtgctcgcca gacaagttgc cagaataagg ctttctgtga
     9661 attccattcc cgacggtaga gattggcaag ctgttcgtgc attccgcttg ccagacccag
     9721 aagcgttgcg aggatcagca catttaagat tcggcgtttg ggcaggagct ccaagacccc
     9781 ggcgaggaga aggctggcgc ccaggttaaa ggctagggtg aagcgatccc atggaaaacg
     9841 cagctcgatc ggtagatttg ttacccaaaa tgggatgcct gcaaacagta aacctgccag
     9901 accaaggaat atggcttggc gagaccaatg ccgggtttct ccagtcggtg agccgcctga
     9961 cgtctgcaaa tcgaaccggg atagatatat cattgccaat gcgatcgaca gggctgtgac
    10021 aaaaaggtag agaacggttg gtcccatgcc gtaacttgtt tgtcttggaa tgttgagtac
    10081 ctggccccac gctattaggc ttgattcgac gatgtcttgc gccactgttg ttacgatctc
    10141 tcgtatggca attatgggcg attcgcttat tttatcgaaa atgcgtacct ggccgcgggg
    10201 agagacgtgg atgaacaagc gccaaatcag aaatgccgca atcaccgcga caaatggcag
    10261 ccagcgaatt gttacactag ttaggcgttt ggtaagaccg ttgccttttg gttggaccac
    10321 caaccaaaga ataattgggc gtagcagttc gagcccgaag aagtattcgt cggtgaacat
    10381 gcaccaggca gaaagaatgc aggagaggga aatcagcggc caaaagtaca tcggcttgcg
    10441 gatcgcccaa atcatggttc ccatcgaaag gagaaaggtc gctaggacca gccaggtgtg
    10501 actgtacgtg acggcaatgt actgttgcag gaagccagga taggcggcaa acaggagcgc
    10561 ggtccaggtc acttggcggt catggttagg ccagagcacc cttagcatcc accacagtgc
    10621 aaggcagctc agccagcgcg agagcagacc gaaaatttgc caggcaagca tcgattcccc
    10681 cacaattgac gaagtgagca tgaaaaacca gcccagagtt gggcgatcta ctgcgaacgc
    10741 atcgataaac ccccttgggc cgagcacatg aagataataa atcgaaggcc aatcatccca
    10801 ataaaa
//
LOCUS       DBNGSX010000483         2745 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_810225, whole genome shotgun sequence.
ACCESSION   DBNGSX010000483 DBNGSX010000000
VERSION     DBNGSX010000483.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_810225"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="AC16CB_14335"
     CDS             <1..265
                     /locus_tag="AC16CB_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343696.1"
                     /translation="IPSIILGILMYRKARGLRIGSGLLAISGMLSLVALLGVAIRNAW
                     LASTTLLSGAVSLVALILIGLYFLRQSALWSSDHDERRVVGEQ"
     gene            complement(312..791)
                     /locus_tag="AC16CB_14340"
     CDS             complement(312..791)
                     /locus_tag="AC16CB_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HIR3343697.1"
                     /translation="MGKQLSGSAQKVQEALKNLGFTLDVVELPNSTRTAVEAAQAVGC
                     QVGQIVKSLVFKAKRSQRPILVVASGSNRVNEKAIEVLIGEPLGKADADFVRQRTGFV
                     IGGVPPVGHVEPLQTFIDQDLLGYAEIWAAAGNPNAVFRLTPEDLIRMTSGVVVKIT"
     gene            complement(845..1834)
                     /gene="corA"
                     /locus_tag="AC16CB_14345"
     CDS             complement(845..1834)
                     /gene="corA"
                     /locus_tag="AC16CB_14345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HIR3343698.1"
                     /translation="MIRSLHITRDGLVKTDLPLGAFSEALRDQDGLLWVDFEGTPPET
                     DEPILRDVFGFHPLAVDDALQESHVPKVDDWGEYLYIVLHAVIFDRTDGGHVDTLELD
                     VFLGRNFMVTHHDQNIAAIERVWSASQRDERLWKNGVDHILYRLTDEVVASYMPVVEE
                     IDEAVDRAEEEVFERATPETLSKIFALKRAVLHLRRIIGPQREVLNKLARDDYPAIDT
                     QARVYFRDVYDHLVRLHDITESIRDLVSGTLDTYLSVINNRMNDIMKTLTIITTMFMP
                     ISFVVGFFGMNFFQPVAASLTPWADTPVFTITLAITILTPLLMYWWIRRRGWM"
     gene            1988..>2745
                     /locus_tag="AC16CB_14350"
     CDS             1988..>2745
                     /locus_tag="AC16CB_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIR3343699.1"
                     /translation="MFTSVVEAIGNTPLVELSRISRDLNGRILAKLEYLNPGFSKKDR
                     IARQMIEEAEGSGALKPGQTVVELTSGNTGTGLAIVCTVKGYPFVAVMSRGNSMERAR
                     MMSALGAEVVLVDQLPDSKPGYVSGGDLELVEAAAQRIVRERGAFRADQFNLEGNFRA
                     HFLHTGPEILSQSAGNIDAFCDFAGTAGSFAGCAAAFKEFNPEIKCYLVEPEGAAVLA
                     GKPVTDPNHPIQGGGYAIRELKLVRNGHVDGYIQV"
BASE COUNT          573 a          731 c          822 g          619 t
ORIGIN      
        1 catcccgtcg atcatcttgg gtatcttgat gtaccgcaag gctcgaggtc tgcgcattgg
       61 cagcgggctg ctggcgatca gcggaatgct gtcgttggtg gctctgctcg gcgtcgccat
      121 cagaaacgct tggctggcgt caaccacgct gctcagcggt gctgtcagtt tagtggcttt
      181 gatccttatt gggctgtact tcctcaggca atccgcgctg tggtcttctg accatgatga
      241 gcgacgggtg gtgggggagc agtgataccg tcgcgtcaat tgtaaaaaga cacatttcgg
      301 aaaaagaacg cctaggtgat tttaacgacc accccgcttg tcatgcgtat caggtcttcg
      361 ggtgtcaggc ggaagacagc gttaggattg cccgccgctg cccagatctc tgcatagccc
      421 agcaggtcct ggtcgatgaa tgtttgtagg ggttcgacat gaccgacggg agggacgccg
      481 ccgatgacaa atccggtgcg ctggcgcaca aagtcggcgt ctgccttgcc cagcggctcg
      541 ccgatcaaca cttcgatagc tttctcgtta acgcgattgg agccgctagc gaccaccagg
      601 atggggcgct ggctgcgctt ggctttgaag accagtgatt tgacgatctg gcccacctgg
      661 cacccaacag cctgggctgc ctccacggcg gtgcgggtcg aattaggcag ctcaaccacg
      721 tctaaggtga agcccagatt ttttaaggcc tcctgaactt tttgtgcgct gccactcagt
      781 tgttttccca ttggcatcct ctctagataa tatttctcgc gcgtctagta tttgcgcgtt
      841 gtaatcacat ccagccccgc ctacgaatcc accagtacat gaggaggggg gtcaagatgg
      901 taatcgccaa ggtgatggta aatacgggcg tgtctgccca aggcgtcagc gaggccgcca
      961 ctggctggaa aaagttcatc ccgaagaaac caacgacaaa tgagatgggc atgaacatgg
     1021 tggtaatgat ggtcagggtt ttcatgatgt cgttcatgcg attgttgatc accgacaggt
     1081 aggtgtccag cgtgccgctg accagatcgc ggatgctctc ggtgatatcg tgcaggcgta
     1141 ccaggtgatc gtacacatcg cggaagtaga cgcgcgcttg ggtgtcgatg gcagggtaat
     1201 cgtcacgcgc tagcttgttg agcacctctc gctgcgggcc gatgatgcgg cgcaggtgca
     1261 gcacggccct tttcagggca aatattttgg aaagcgtctc tggcgtggcg cgctcgaata
     1321 cctcctcctc ggcgcggtcg acagcctcat cgatctcttc caccacgggc atgtagcttg
     1381 ccaccacctc gtcggtcagg cggtacaaga tgtgatcgac accgttcttc cacaggcgct
     1441 cgtcacgctg gctggctgac cagactcttt caatggccgc gatgttctgg tcatggtggg
     1501 taaccatgaa gttgcgcccc aggaacacat ccagctccag cgtgtccaca tgcccgccat
     1561 ctgtgcgatc gaagatcacg gcgtgcaaga cgatgtaaag atactcgccc caatcgtcca
     1621 ccttggggac gtgtgattct tgcagggcgt catccactgc cagggggtga aagccaaaga
     1681 catcccgcag aatgggctcg tcggtttcag gcggtgtccc ctcgaaatca acccacagga
     1741 gaccatcctg atcgcgcagt gcttcagaga aagctcctag aggaagatcg gttttcacca
     1801 ggccatctct tgttatgtgt agggaacgga tcatgggcat gctcctcgcc agacacgggt
     1861 tggatgatga tatataggga gagtctatcg cagcgatata cgttgtgtca atcatggaat
     1921 gcaaaatcgc acgctcctct ggggtgggag tttgtcgtta taatcgttga aaagaaggag
     1981 gactaccatg ttcacctccg ttgttgaagc cataggaaac acgcctctgg tagagctttc
     2041 ccgaatttcc agggatctta acggtcgcat cctggccaag ctggaatact tgaaccctgg
     2101 attttccaag aaagaccgca ttgcccgcca gatgatcgag gaggccgagg gaagcggggc
     2161 gctaaaacca ggccagaccg tggtcgagct gaccagcggc aataccggca cgggcttggc
     2221 gattgtctgc acggtgaagg gctacccctt tgttgcagtc atgtcacgcg gcaattcgat
     2281 ggagcgggcg cgcatgatgt ctgcgttggg cgctgaagtg gtgctggtgg accagttgcc
     2341 agattccaag cctggctacg tctcaggtgg cgacctggag cttgtggagg cagcagccca
     2401 acgcatcgtg cgggagaggg gcgcctttcg agccgatcag ttcaacctgg agggcaattt
     2461 ccgcgcccat ttcctgcaca ccgggccgga gatactcagc caatcggcgg gtaatattga
     2521 tgctttttgt gatttcgctg gcacggccgg ttcttttgcc ggatgtgcag cagcattcaa
     2581 ggaattcaac cctgagatta agtgctacct ggttgagcct gagggcgcag cggtgctggc
     2641 tggcaagcca gttactgacc caaaccatcc catccagggc gggggctatg ccataaggga
     2701 attgaaactt gtgcgtaatg ggcacgtgga cggatatatc caggt
//
LOCUS       DBNGSX010000484         6021 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_602802, whole genome shotgun sequence.
ACCESSION   DBNGSX010000484 DBNGSX010000000
VERSION     DBNGSX010000484.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6021)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_602802"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="AC16CB_14355"
     CDS             complement(<1..865)
                     /locus_tag="AC16CB_14355"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3343700.1"
                     /translation="MKKIHLITLSLVVIFSLVTSSVLAQDAEVANPAETELAEHVPGE
                     LLLRFSPGMDSAQVASKMAEMGVTHKREIPAIGVRLVKLPPGLSVERAVARFSRLPGV
                     EFAEPNYILHIAETSQAELDPTWGLTKIYASEAWEALDDATEQNEVLLATVDTGIDFS
                     QSDLSSRKWANVDEIPGNGIDDDKNSFVDDTWGWDFVNNDNDPIDDNMHGTAVSGVMA
                     ATGTEAATGTDVKGVCPWCKVMAVKVLGANGSGTLDVVANGITYAAANGARVINLSLA
                     GAAGSVTLQSAV"
     gene            complement(1459..2463)
                     /locus_tag="AC16CB_14360"
     CDS             complement(1459..2463)
                     /locus_tag="AC16CB_14360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3343701.1"
                     /translation="MKITSEADVDFYLEAYVGSAALGAALELGLLWLLAEKPLSAAGI
                     AQELNIPIKRCRYWLQILSNMGLIDQVPGGYVPSSIAKSAILNTFDQEIWALSAREAR
                     ERFPSMLDLAVHIREPGSTWASQGRTPPDYVSKMVEDPQTARTFTRMLYEVHRNLANQ
                     LALHLDMTGVFRLMDLGGGSGVVSMALLRKHPELTSVVVDIENVCVVGQEIARENSLA
                     DRITYYAADFLHDELPGEFDMVLVCDVGVFDEALFRKLWAALKLGGRFVIVEHFAPEE
                     NVAPPQRLHWAFRDSMVDPDFSVYTLHQIKEQLSQVGFELLPMDISLPGGRIVIQARK
                     "
     gene            complement(2828..4252)
                     /locus_tag="AC16CB_14365"
     CDS             complement(2828..4252)
                     /locus_tag="AC16CB_14365"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3343702.1"
                     /translation="MKRIMFPAIVVLIFTVLVSSCTSAPGDQAGTAVEESSPATKPAG
                     PSETPVPPSSTPIPTDTSVPTETPIPTDTPTSLPTETPTITPTFSPDTLTFTAGEPVR
                     IGYLLWESNPIGLDSKRAIELAIQGFGGELFGHPIELIGFDEECSELGGQRGAQLLGL
                     DDSVVGIIGSSCSVAARRAAPIVSDAGRVLISPSNTSPELTDPDGRAAGYFRTVPNDT
                     FQANAVAQYAYDQLAVRKMATIYTQNQKYQKTLAQHVCQSFTSLGGECVLEKAIASNA
                     TYMVPIVNNILDAKADAIHLSHFSPQVAAEFIAEVRKTKGLENVPIFVFEGLNTPDFL
                     QKAGENAVGIFVSTTAMDFDHSTDSYQGFLSAYREKYGEDPISQFHGFAYDAAMILLD
                     AMYKVAVQSGDGSLLIDPLAVRQAISTLVDFPGLTGSLTCSPQGDCSPLVDGRVYRFE
                     DADPNTFRPGPPESLSSNPVQVWP"
     gene            complement(4321..>6021)
                     /locus_tag="AC16CB_14370"
     CDS             complement(4321..>6021)
                     /locus_tag="AC16CB_14370"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012509447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004467 - long-chain fatty acid-CoA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HIR3343703.1"
                     /translation="RRCMMESKWWLRHYDEGVPHTLQPYPESTLLDIVKETSTQRPEH
                     TALLFKGSKITYAELDRLSAAFANALIDLGVKKGDRVALMLPNSPQFIISLLGVWKAG
                     GVVVPLNPLYTEHELQHALADCGAETIVVLTGFYGKVKAIQKQLNIRNIIATSIKEYL
                     PPVSRVLFTVLREKKDGHRITLQAGDFWFPDLIRKFSNAPQPNVQIQPDELAILLFSG
                     GTTGTPKAAMGTHKALLISGTQIRAWFNRTLIDWDDIIVLNMPLFHAYGLAGVFSTGL
                     VGHNSFVVVPNPRDLDDILATIEKVKPAFLPGVPTLFTAILNHPKVKSGQVDLKCLKL
                     SISGASPLLKETKERYESVTGGRIVEAYALTETMLGATVTPILGEYKPASVGFPLPDV
                     EIRITDAETGRGELEPGQVGEILVRAPQLMIGYWHNPGETANILRDGWLYTGDLGYLD
                     EEGSLFIVDRKKDVIKPGGFQVWPREVEEIIASHPAVSEVCVGGVPDPYQVEAVKAWV
                     ALRDGQQVTAEELRDYCREKLAGYKVPKFVEIRDSLPKSTVGKILRRELLAEEVALQK
                     "
BASE COUNT         1457 a         1646 c         1506 g         1412 t
ORIGIN      
        1 ccaccgcact ttgcagggtc accgagcccg ccgcgcctgc caggctgagg ttgatcacgc
       61 gtgccccgtt tgcagccgca taggtgatgc cattggcaac gacatccagg gtgccgctgc
      121 cattcgcgcc tagcactttg accgccatga ccttgcacca ggggcagacg cccttcacat
      181 cagtgccagt tgcagcctca gtgccagttg cagccatcac gccggagaca gccgtgccgt
      241 gcatgttatc atctattgga tcattgtcat tgttgacgaa gtcccagccc caggtgtcat
      301 ccacaaagct gttcttgtcg tcatcaatcc cattgccagg tatttcatcg acgttagccc
      361 acttccttga actgagatcg ctctggctga aatcgatgcc cgtatcaacg gtcgccagca
      421 gcacctcgtt ttgctcagta gcatcatcta aagcctccca ggcttctgaa gcgtaaatct
      481 tggtcagtcc ccatgtgggg tcgagttctg cctgggaagt ctctgcaata tgcaggatgt
      541 agttcggctc ggcaaattcg accccaggca ggcggctgaa gcgagcgacc gcacgctcaa
      601 ccgataaacc tggaggcagc ttgaccaggc gaacgcctat ggccgggatc tcgcgtttgt
      661 gggtgactcc catttctgcc atcttgctag ctacctgggc ggaatccatc ccgggagaga
      721 aacggagcaa caactcccca gggacatgtt ccgccagctc agtctccgcc gggttggcaa
      781 cttcagcatc ttgcgccaac acagatgacg tgacgaggga aaatatcact accagactaa
      841 gagtgatgag gtgtatcttt ttcatgttcc cctcctaaag aaatggtcct gacaagagaa
      901 tgggagtatg caattggtaa gaaaagcccc tgacatcctg tcagcggctt cttgaaaatt
      961 aaggtgttta tcagacaatg aattcggttg aaatccgccc gtaaagcaac cgcaatgtct
     1021 tgctaatatt acaacaacgt tactgcgaaa ttaaaggcaa aaatcaactt tactaccatt
     1081 attacgataa ttcgccgctg ttacctcacc atttatagta tagcgaaaat taacataaaa
     1141 atcaacttcg cgaactgcaa attacgtaat tgtaagatta aggtccttta ggattttttt
     1201 gtcccacctt agtgctcacc accgcccgac acccatttgc actgctggta agggagacaa
     1261 aatcgctggt ttaaaacgag tacagcatca aaaatgtgct ctctcttttc ctggctagat
     1321 actggcgaat aatttgtatg gcaatggcga cccggaggct catgatcgcc gtggaaacca
     1381 gtgggggatg agagtcatgc tcagacccac tactccgttc taactcatta atctgcgcga
     1441 gtgaatcaaa aaggcggatc acttgcgggc ttgaatcaca atccgtccac cgggcaagga
     1501 gatatccatt gggagaagct cgaaccctac ctgcgacaat tgttccttga tctggtgaag
     1561 agtgtacaca ctaaaatcag gatctaccat cgaatcccga aatgcccaat gcaggcgctg
     1621 cggaggtgcc acattttctt cgggcgcaaa gtgttctacg atcacaaaac gcccgcctag
     1681 ttttaaggca gcccataatt ttcggaagag cgcttcatca aagacgccaa catcacacac
     1741 caagaccata tcgaactcgc ctggcagttc gtcatgaagg aaatcggcag cataataggt
     1801 aatccggtcg gcgagtgaat tttcacgcgc aatctcctgt ccgacgacgc agacgttctc
     1861 aatatcgacc accacggacg taagttctgg gtgcttccgc agtaaagcca tagacactac
     1921 acctgaacca ccccccagat ccatcaatct gaaaaccccg gtcatatcca gatgcaacgc
     1981 cagctgattc gccagatttc gatgaacctc gtagagcatg cgagtgaatg tacgcgctgt
     2041 ctgagggtcc tcaaccatct tgctcacgta atctggtggt gttcgccctt gggatgccca
     2101 ggttgagcct ggctcgcgga tgtgcacggc taagtcgagc atagatggga accgctccct
     2161 cgcttcgcgg gctgacaatg cccagatttc ctggtcgaaa gtgttcaaga tggcgctctt
     2221 agcaattgac gacggcacat atcctccggg gacttgatca atcagcccca tgttactcaa
     2281 tatttgcagc caatagcggc aacgttttat gggaatattc aactcctgtg caatccctgc
     2341 ggcacttaac ggcttctcgg cgagtaacca taataatcct aattctaaag ctgctcccaa
     2401 tgcggcagag ccaacatacg cctctagata aaaatcgaca tcagcttccg aggttatttt
     2461 catctcgctt ctccatttgc atcctgagtc tcgatttggt gcgaaacatc attgcaactg
     2521 ccgtttcaac cgcaattgca caacgcttgg agtgcattat ttatattttg cacccgaaac
     2581 caggcaccga cctaaacatt taatggtcca atcgagccct atgtgccctg ccagagatca
     2641 gaaagacgca atcggcgaac cactttaggc gcaagtggac ggactaaatg agtgagaacg
     2701 caaggcggcg acccaaatac agatagaagt tacgcagtta gggaggtgat ggggggttgg
     2761 ggcgggtcat aacccggcca aactccccct ctttcccctt caactgcgta agtcctaaca
     2821 gagatattta tggccaaacc tggaccggat tagaactcag cgattctggc gggccgggtc
     2881 tgaaggtatt agggtctgca tcttcaaaac gatacaccct gccatccacc aagggggaac
     2941 aatccccctg tggggaacag gtcaacgatc cagtcaggcc tgggaaatca accagcgtcg
     3001 atatcgcctg gcgaacagcc agagggtcaa tcagcaaaga tccatctccg gattggacag
     3061 cgaccttgta catcgcatcc agtaatatca tcgcggcatc gtacgcgaat ccatggaatt
     3121 gcgatatcgg atcctcgcca tatttttccc gatatgcgga taggaaaccc tggtaactat
     3181 ccgtgctatg atcaaaatcc attgcagtgg tggacacgaa aatcccgacg gcgttctcgc
     3241 ctgccttctg gaggaaatcc ggggtattta atccttcaaa gacaaagatg ggcacattct
     3301 ccagcccttt tgtttttctt acctcggcta taaattccgc tgcaacctgg ggagaaaaat
     3361 gagacaggtg gatcgcatct gccttcgcat ctaggatgtt attaacaatc ggcaccatat
     3421 aggtggcgtt gctggctatg gctttctcca agacgcactc acctcccagg ctggtgaagg
     3481 actggcagac gtgctgagct aatgtctttt gatatttctg attctgggta tagatggttg
     3541 ccatcttgcg cacggctagc tggtcgtatg catactgagc gacggcgtta gcctggaaag
     3601 tgtcgttggg tacagtgcga aagtaaccag ccgcacgacc atcagggtcg gtgagctcgg
     3661 ggcttgtgtt ggacggcgag atcaaaaccc tgcccgcgtc cgagacaatc ggggcagctc
     3721 ttctagccgc tacagaacaa ctcgagccga tgatcccaac cacgctatcg tccagtccta
     3781 gcaactgtgc gcctcgctgt cctcctaact cggagcactc ctcatcaaat ccgatcagct
     3841 caatagggtg cccgaacaac tcgcctccaa atccctggat agcaagctcg atggcgcgtt
     3901 ttgaatccaa gccaatggga ttgctttccc aaagcaggta tccaatcctc accggctctc
     3961 ccgccgtgaa ggtcagtgta tcaggagaga aggtcggtgt gattgtgggg gtctcggtcg
     4021 gcagagaggt cggggtgtct gtaggaatgg gcgtctctgt agggacggag gtgtcggtgg
     4081 gaatgggggt gctgcttggc gggactggtg tttcgctcgg ccccgcaggt ttggtcgccg
     4141 gcgatgattc ctcaactgcc gtgcctgcct ggtcgcccgg agcggaggta caacttgata
     4201 caagcacagt gaatattaat acaactatcg cggggaacat tattcgtttc attggttgca
     4261 cctccggcga atgactatcg tccagtgatc tccgagaatt tttcaatccg atcaagtcat
     4321 ctatttctgc aacgccacct cctcggctaa taattcgcga cgcaagatct taccaacggt
     4381 ggatttgggc aggctgtctc gaatttccac aaactttgga actttatacc cagccagctt
     4441 ctcgcggcag taatcgcgca gctcctccgc tgtgacttgc tgcccgtcac gcagagcgac
     4501 ccaggctttg acagcttcca cctggtaggg atctggcacg ccacccacac agacttccga
     4561 aaccgctgga tgagaagcaa tgatctcctc cacttcgcgc ggccacacct ggaagcctcc
     4621 cggcttgatg acatccttct tgcggtcaac aatgaagaga ctgccctctt catccaagta
     4681 acccaggtca cccgtgtaga gccagccatc tcgaaggata ttggcggttt cgcccgggtt
     4741 atgccagtaa ccgatcatta gctgcggggc acgcaccagg atctctccca cctgccccgg
     4801 ttcaagctca cctcggccgg tttccgcgtc ggtgatgcgg atttctacat cgggtagcgg
     4861 aaacccaacc gaggccggct tatactcgcc cagaattggt gttaccgtcg cccccagcat
     4921 agtttctgtc agcgcataag cttccacaat gcgcccccca gtcacgcttt catagcgctc
     4981 cttggtttcc ttcagaagag gtgatgcacc ggatatgctg agcttgagac atttgagatc
     5041 tacctgacca gacttcacct taggatggtt gagtatcgcg gtgaacaggg tgggcacacc
     5101 cggcagaaag gcaggtttga ccttttcgat ggtagccaat atatcatcta gatcgcgcgg
     5161 gttgggcact acgacaaacg aattgtgacc gaccaggccg gtagaaaaca cccccgccaa
     5221 gccatatgcg tggaagagcg gcatattgag cacgataatg tcatcccaat cgattagtgt
     5281 gcggttgaac caggcgcgaa tctgcgtgcc ggaaatcaga agcgccttgt gtgtgcccat
     5341 cgccgctttg ggtgtgccgg ttgtaccccc ggagaaaagc agaatagcca gctcatccgg
     5401 ctgtatctgg acattcggtt gcggcgcgtt agagaatttc cggatcaagt ctggaaacca
     5461 aaaatcgccc gcctgaagcg ttatccgatg accgtctttc ttctctctta aaacagtgaa
     5521 caatacgcgg gatacaggcg gcaggtactc tttgatgctt gtggcgatga tattacggat
     5581 attgagttgc ttctggatgg ctttgacttt gccgtaaaac cccgtaagca ccacgatggt
     5641 ctcagcgccg caatctgcta aggcatgctg cagctcatgt tccgtgtaaa gcggattcaa
     5701 tgggacgaca acacctcccg ctttccatac accgagcagg gagataatga attggggtga
     5761 attgggcagc atcagtgcga cacgatcgcc tttctttacg cccaagtcta tcagggcatt
     5821 tgcgaaggca gcgctcaagc gatccagttc agcatatgta attttagagc ccttaaatag
     5881 taacgcagta tgctcgggac gttgtgtgct agtttctttg acaatgtcaa gcaatgtcga
     5941 ttcgggataa ggttgcaatg tgtgtggaac cccctcatca tagtgccgaa gccaccattt
     6001 actctccatc atgcacctcc t
//
LOCUS       DBNGSX010000485         2542 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1087578, whole genome shotgun sequence.
ACCESSION   DBNGSX010000485 DBNGSX010000000
VERSION     DBNGSX010000485.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1087578"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="AC16CB_14375"
     CDS             <1..234
                     /locus_tag="AC16CB_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343704.1"
                     /translation="VVLNRTLAYQIAEFLGSEETDDWIGKRITLFPQPMTVAGKKVTA
                     IRARAVKGAPEALGEAIPAGLVEEDEDGEGIPG"
     gene            331..2061
                     /locus_tag="AC16CB_14380"
     CDS             331..2061
                     /locus_tag="AC16CB_14380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR3343705.1"
                     /translation="MNPRSAWSTKNWSELSVKGRTAWLQWIMLIVVGLAIAVPRLKAL
                     NRLVTPDEHLWLARSANFYMAISQGKLEWTYLSEHPGLTVMWAGTGGFLVRYPQYRHD
                     GPDRVDSNEFNRYIQNAKGVSALDLLVASRVFMILGHTLVLLVSFCYAIRIISPFPAL
                     ISFLLIAFEPYHVALTRVLHLDGLLGNMLLLSLLAIISFLQGRRTRDLLVSAIAAGLG
                     LLTKSPGILIVPVVGLLTLFALRKDGLGKQERSIPRLLGSTIRISAIWGLVLIITFVA
                     LWPAMWVSPVQTIRTIVLEGWTHYETESKTPRFFNGEIFTSDQFGIKYWYFYPVTYLF
                     RSSPVVLFGLILAAWAIVKKRHPMDSSSIQFVLLSLLLFIGVFTAAMATSDIQHDRYL
                     LPVYPPFDIIAGLGWSSLILSWSRKTAGGFQRYVAPMVALLIIVIHGMLSLSVYPYYL
                     SYYNPLLGGARKAQKILSIGWGEGLDQAAEYLNKKPRAGQLEVLSYYASGCFSYYFNG
                     RVRAVSFAKDLTEDDWQKFLNSDYVVIYISQRQREIASPILDYVADLTPEYTVWINGL
                     DYAQVYKIHE"
     gene            2102..>2542
                     /locus_tag="AC16CB_14385"
     CDS             2102..>2542
                     /locus_tag="AC16CB_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343706.1"
                     /translation="MQIRSRQYFTIAVIIVINIAIGCIVVTDYGESRDEHLRIDYAER
                     SLAAYTHGKRVSVDEKGPSYVMLALVGSRALRAVWGSLHPIEAWHFMHFLSFLMGVYF
                     LYLICRHYMSEGAALATVLLFNTQPLLWGHAWINPKDIPFMSFFL"
BASE COUNT          557 a          672 c          637 g          676 t
ORIGIN      
        1 gttgtgctca accgcacgct ggcttaccaa atcgccgaat tcctgggcag cgaggagaca
       61 gacgattgga tcgggaaaag gatcacgctc ttcccacagc caatgaccgt ggcaggtaag
      121 aaagtcactg ccatccgagc ccgggcagtc aagggcgctc ccgaggcttt aggtgaagcc
      181 attcctgccg gcctggtaga agaggatgaa gatggggagg gaattcctgg gtaattatca
      241 cggagtctgg tagggcggtc tgggcgaagc actgatccat ctcctcttac ctctccaaac
      301 gcgcaacttc tgagattata atgaagccta atgaacccca gaagcgcttg gtcaaccaaa
      361 aactggagcg aattatccgt caaaggtcgc accgcatggc ttcaatggat tatgctgatc
      421 gtggtaggtc ttgccatcgc tgtgcctcgt ctaaaagcct tgaatcgcct ggtcacgcca
      481 gacgaacacc tgtggctggc acgctctgcg aatttttaca tggctatcag ccagggcaaa
      541 ctggagtgga cataccttag cgaacaccct ggtcttaccg tgatgtgggc tggcacaggt
      601 gggttcctcg tccgctaccc gcagtatcgg catgatggcc ccgacagggt cgactccaac
      661 gaattcaatc gctatatcca aaatgccaaa ggggtctcgg cgttggacct cctggtagct
      721 agccgcgtct tcatgatcct tgggcatacg ttggttctac tagttagctt ttgttatgct
      781 atccgcatca ttagtccatt tccggcatta atctccttcc tgctgattgc cttcgaacct
      841 tatcacgtag ccttgacacg cgtgctccac ttggatggtt tattaggcaa catgcttctg
      901 ctctccttgc tagcgatcat ttcatttctc cagggaagac gaaccagaga tttgctggtt
      961 tcggctatcg ccgctgggct gggattgctg accaaatcgc caggaatatt gattgtgcca
     1021 gttgttggcc tgttgaccct gtttgcactc cggaaagatg gattgggcaa acaagaacgc
     1081 tcgattccaa ggctcctcgg gagcaccatc cgaatttcgg cgatctgggg tctggtcttg
     1141 ataatcacct ttgtcgccct ttggcctgcg atgtgggtca gccctgtaca aacgatcaga
     1201 acaattgtcc ttgagggctg gactcattac gaaacagaat ccaaaacgcc gcgcttcttt
     1261 aatggggaga tctttaccag cgatcagttc gggatcaaat attggtattt ctatccggtc
     1321 acctatctat tccgcagtag tccagtcgtg ctgtttggtc tgatcctggc agcctgggca
     1381 atcgtaaaga agcgtcatcc catggactct tcaagtatac agttcgtcct gctaagcttg
     1441 ttgcttttca tcggagtttt cactgcagcc atggcaacca gcgatatcca gcatgaccgt
     1501 tatctgctgc ccgtgtatcc accctttgac atcatcgctg ggctgggttg gtcatcttta
     1561 attttatcct ggtcgaggaa aacagcaggc ggtttccagc gttatgtagc ccccatggtt
     1621 gcgttgctca tcatcgtcat tcacggcatg ctctcgctta gtgtctatcc ctactatctt
     1681 tcttactata accccttgct gggaggcgcc cgcaaagccc agaaaatttt atctattggc
     1741 tggggtgaag ggttggatca ggcagcggaa tacctgaata agaagcccag agcgggccaa
     1801 ttggaggtgc tctcttatta tgcctcggga tgtttctctt actatttcaa cggacgagtc
     1861 agggcggtat cgtttgcgaa ggatttgacc gaagatgatt ggcaaaagtt tcttaattcg
     1921 gattatgttg ttatttacat atcccaacgg caacgcgaaa ttgcctcccc catcctagat
     1981 tacgtagccg acttaactcc agagtacacg gtctggatca atggtttgga ttacgcccag
     2041 gtctacaaaa tccacgaata gctgacccaa aatattaacc cgttcgaccc ggaatctgtt
     2101 cttgcaaatt cgctcccgcc aatactttac gatagcagta atcatcgtta tcaatatcgc
     2161 catcggttgc atagtggtaa cagattacgg cgagtcgcga gatgagcacc tgcgcattga
     2221 ctacgccgag cgctcactgg cagcatatac ccacggcaag cgggtcagcg ttgacgagaa
     2281 gggtccctct tatgtcatgc tggcactggt cgggtctcgt gcgctgcgcg cggtgtgggg
     2341 cagcctgcat cccattgagg cctggcactt catgcatttc ctctcctttt taatgggggt
     2401 ttattttctc tacctcatct gccgccatta tatgagcgag ggggccgcat tggcaactgt
     2461 cctgctcttt aatacccagc cgctgctctg gggtcacgcc tggattaacc ccaaagacat
     2521 cccttttatg tccttcttcc tg
//
LOCUS       DBNGSX010000486         3820 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_602927, whole genome shotgun sequence.
ACCESSION   DBNGSX010000486 DBNGSX010000000
VERSION     DBNGSX010000486.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_602927"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3008)
                     /locus_tag="AC16CB_14390"
     CDS             complement(<1..3008)
                     /locus_tag="AC16CB_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cellobiose phosphorylase"
                     /protein_id="HIR3343707.1"
                     /translation="MSIFKLDENNRFIINDYQQQRPFSSTLPGIAGPMGIPTWVFYVN
                     RGQAITSFGVENKDHPLMEFQPANKAYQVTPFLGFRTFIKILHADQQVLYEPFVAGSR
                     DREQRMIISLNELELHERYPTRDLQVEILYFILPEEKIAGLIRQVKITNLASEPVQLE
                     ILDGLPVIVPYGVSNNDLKERNRTVEAWMQVCNLENNMPFFNLSSTLGDTVEVKPVQP
                     GNFALAYAVQGNFKQKLAAILSPEAVFGENTSLNYPTRFERHSLSEMMQQPQFNRGKT
                     PCAFFGYPAKLAPAESVTLYSLFGYASNIEVLKEVAPRLGADGYFSGKRSFANKLVSE
                     ITAPIETRTSSPVFDAYCKQTFLDNVLRGGWPVHLGTVEKPITYHIYSRKHGDPERDY
                     NAFFLAAEYYSQGNGNYRDVNQNRRCEVLFDPRVGDANVHSFMSLIQLDGYNPLVVKG
                     SWFWIPPERRNSILKLTVQPERMEQFLDKPFTPGSLLKWIDENEVGLHISTSIFLQEA
                     LQQAEQYFDAEFGHGYWIDHWTYNLDLINSYLAVFPDRGSELLFGKGDLPFFESPVFV
                     QPRSKKYVLAGEHPRQYGAVVRDEEKAALITARRDYPNLMRTQKGHGSVYRTSLYAKL
                     VLLALIKFATLDPLGLGIEMEAGRPGWCDALNGLPGLFGSSLSETFELARLLIFLHEH
                     LPKSMSGSVDLPVEVSDLLQSVVNCLRAYHNSDQKERDFIYWDSVSNARELYREKIRF
                     GIDGEVVPITFQVLAGYLSLFLDKVRSGIERALELNGGIPPTYLTYDVEKYDILYGID
                     NKPIKDAKGRSYIRALRFQPRFLPVFLEGPVRMLKTLPDSHAARQLHQQIKGSTLYDR
                     SLQMYRTNAPLDNEPVEIGRLRAFTPGWLENESIFLHMEYKYLLELLRAGLYEEFFED
                     FRNALVAFQDAAKYGRSPLENSSFIVSSAHPDKNLHGAGFVARLTGATAEFLNMWNIM
                     MAGQNPFFIDKDELCLRLQPALPHWLFDEAG"
     gene            complement(3093..>3820)
                     /locus_tag="AC16CB_14395"
     CDS             complement(3093..>3820)
                     /locus_tag="AC16CB_14395"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HIR3343708.1"
                     /translation="PIPTLQGWNLIWQDEFDGDTIDPANWTFDIGAGGWGNGEAEFYT
                     SRPENSRVENGMLVIEARQEKYEGSYYTSARLKTQGLQNFQYGRVEARLKVPSGKGLW
                     PAFWMLGSNFNGSNWPDCGEIDIMEYIGKEPDLIMGTLHGPGYSGALGYTKWNRQTYN
                     IADDFHTYAIEWEPNEIRWYYDGAHYHTVTPTDIGDREWVFDHPFFIILNLAVGGQLP
                     GPVGLDTEFPTQYYVDYVRVYQR"
BASE COUNT          972 a          948 c          921 g          979 t
ORIGIN      
        1 ccagcctcgt cgaacagcca atgagggaga gcaggttgca aacgcaggca gagctcatct
       61 ttatcaatga aaaatggatt ctgacctgcc atcatgatat tccacatgtt tagaaactct
      121 gctgtcgctc cggtcaggcg agcgacaaag ccggcaccgt gcaggttttt gtcggggtgg
      181 gcgctgctga caataaagga tgaattctcc aaggggctac gtccgtattt ggctgcatct
      241 tgaaaggcaa ccaatgcatt gcgaaaatcc tcgaagaatt cttcatataa cccggctcgt
      301 aagagctcga gcaggtattt gtactccatg tgtaaaaaga tggattcgtt ttccaaccaa
      361 ccgggtgtaa acgctcgtaa gcgcccgatc tccacaggtt cgttatccag gggagcgttt
      421 gttctgtaca tttgtaggga acggtcatac aacgtgctgc ccttaatttg ttgatgtaac
      481 tggcgagctg catgagagtc tggcagtgtt ttgagcatcc gcacaggtcc ttctaggaaa
      541 acaggtaaaa accttggctg aaaccggagg gcgcggatgt acgaacgccc ttttgcatct
      601 tttataggct tgttatctat tccatacagg atgtcatact tctcgacatc atacgtgagg
      661 taggtaggtg gaataccccc atttaactct aaggcacgct cgataccgct tcgcacttta
      721 tccaggaata gtgataagta tcccgccaat acttggaaag ttattggcac aacctcgcca
      781 tcgatgccaa atcgtatttt ctcccggtag agctcgcggg cattggaaac gctatcccag
      841 taaataaaat cacgctcttt ctgatcagag ttgtggtaag cccgcaagca attgacgaca
      901 ctttggagga gatctgaaac ttctacaggt aggtctacag aacctgacat gcttttggga
      961 agatgttcat gcaagaagat caacaatcgc gccagctcaa aagtttctga caaggatgag
     1021 ccaaacaacc ctggaaggcc attcagcgcg tcacaccatc ctggtcgccc cgcctccatt
     1081 tcgatgccca acccaagtgg atccagcgtg gcgaatttaa tcaacgccaa caaaaccagc
     1141 ttagcataca aactggtccg atacacggat ccatgaccct tctgtgtacg catcaaattg
     1201 ggataatccc ggcgggcggt gatcagggca gctttttctt cgtctcgtac taccgcacca
     1261 tattggcggg gatgttcacc tgccagcaca tatttcttgc tgcgtggctg cacaaagacc
     1321 gggctttcaa aaaatggcaa gtcgcctttg ccaaagagca actcgcttcc ccggtctgga
     1381 aaaacagcca ggtagctgtt gatcaaatcc aggttatagg tccaatgatc gatccaatat
     1441 ccgtgtccga attcagcatc aaaatactgt tcagcttgtt gcagtgcctc ttgcaggaaa
     1501 atactcgtgg atatatgcag acccacctca ttttcatcta tccacttgag taaacttccg
     1561 ggcgtaaagg gcttatctag aaattgctcc atgcgctcag gttggacagt cagcttgagg
     1621 atcgaatttc gtctttcagg tgggatccag aaccaactac ctttcaccac cagcggattg
     1681 taaccatcaa gttggatcag gctcatgaag gagtgtacat tggcatcccc tacgcggggg
     1741 tcaaacaaaa cctcgcagcg gcgattttga ttaacgtcgc gatagtttcc attaccttgc
     1801 gagtagtatt ctgctgctaa gaaaaaggca ttgtaatccc gctccgggtc cccatgtttg
     1861 cgcgaataaa tatgatatgt gattggtttc tcaacggtcc ccagatgaac cggccaacca
     1921 ccgcgcagga cgttatctaa aaatgtttgc ttgcaatacg cgtcaaagac gggcgaactg
     1981 gtcctggttt caattggtgc agttatttcg ctcactaatt tgttggcaaa gctccttttt
     2041 ccggagaaat acccatccgc gcccagacgc ggtgcaacct ctttgagcac ttctatgttg
     2101 ctggcatatc caaagaggct gtatagagta actgactcag caggagccag cttggcagga
     2161 tagccaaaaa acgcacaggg cgttttgcca cggttgaact ggggttgttg catcatctca
     2221 gaaagcgaat gcctctcaaa gcgtgtgggg tagttaaggg atgtgttctc tccaaaaacc
     2281 gcttcagggc ttaagatcgc ggctagcttt tgtttaaaat tcccttgaac agcgtatgca
     2341 agggcaaaat tgcctggctg gaccggtttc acctctacag tgtcgcccaa agtggagctc
     2401 agattgaaga aaggcatatt gttttccagg ttacatacct gcatccaggc ttcaactgtg
     2461 cgattgcgct cttttaggtc attgttagat acaccatatg ggacaatcac tggcagacca
     2521 tccaggattt ctagctggac tggctccgag gccaggttgg taattttgac ctggcgaata
     2581 agcccagcta tcttctcctc gggtaggatg aagtaaagaa tctcaacttg caagtctcgc
     2641 gttgggtagc gctcatggag ctctagctca tttaagctga tgatcatccg ctgctctcga
     2701 tctcgcgagc cagccacaaa aggctcgtaa aggacttgct gatcagcatg gaggattttt
     2761 ataaatgttc gaaaacccag aaaaggggta acctggtagg ccttgttcgc aggttggaac
     2821 tccatgagcg gatggtcttt gttttcaacg ccaaaactgg tgatagcctg gccgcgattc
     2881 acatagaata cccaggtagg aatacccata gggccagcga tacctggcag tgtgctggag
     2941 aatggccgtt gttgctggta atcattgatt atgaagcgat tattctcatc aagcttgaag
     3001 atgctcacat cggattcctg aatttgattc tcttgctgcg gagaagatta tctcccacca
     3061 acgactgttg atgggtagat tggtgaagtg gcttagcgct gataaacccg cacataatct
     3121 acgtagtatt gagttgggaa ttctgtatca agaccaaccg ggcccggcaa ctggccgcca
     3181 actgccaggt tcaggataat aaagaaggga tgatcaaaga cccactcgcg gtcgccgata
     3241 tctgtaggcg tgaccgtgtg gtaatgcgcg ccatcatagt accagcggat ttcatttggt
     3301 tcccattcga tggcataggt gtggaaatca tcggcgatat tgtacgtttg ccggttccat
     3361 ttggtataac ccaaggcccc agaataccca ggcccatgta gcgtccccat aatcagatcc
     3421 ggttctttac cgatgtattc catgatgtca atttctccac aatcgggcca gttactacca
     3481 ttaaaatttg agccaagcat ccagaatgct ggccacaagc ccttgccact agggactttg
     3541 agccgtgctt caacccgacc atattggaaa ttttgcaacc cctgggtttt taaacgcgcc
     3601 gaggtgtagt atgagccctc gtatttctcc tgcctggctt caataaccag catgccattt
     3661 tctacacggg aattttctgg gcgcgaggtg taaaattctg cttcgccatt cccccagccc
     3721 ccagccccta tatcaaacgt ccagttggca gggtcgatcg tatcaccgtc aaattcatcc
     3781 tgccagatta aattccaacc ttgcaaagtc gggatcgggg
//
LOCUS       DBNGSX010000487         3276 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1433871, whole genome shotgun sequence.
ACCESSION   DBNGSX010000487 DBNGSX010000000
VERSION     DBNGSX010000487.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1433871"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="AC16CB_14400"
     CDS             complement(<1..473)
                     /locus_tag="AC16CB_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase-I family protein"
                     /protein_id="HIR3343709.1"
                     /translation="MERPEQKNIKKAQNTGKINLHRAAAPFTASDLPIDDGGRIYHLQ
                     VKPGQIAPDILLVGDPGRAEFIGSNLMRDLELKHEHRGLVTLTGTSEITGEQTTIISP
                     VRTTVSTSGMGTPSLEIVVSELVALNEIDFETRTRKTNFPRLHIIRLGSSGGLQAS"
     gene            complement(632..1153)
                     /locus_tag="AC16CB_14405"
     CDS             complement(632..1153)
                     /locus_tag="AC16CB_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343710.1"
                     /translation="MQIMPTDPVIRAAGGFAILSGIISAIGVVFLIAMFILFTTPYQA
                     QALTFGLLNDICVSVQYLLTIPIALALYRILRPYSPTLILVATIVGILAMLLIIALQL
                     ALIFQVLTFQQQGFWVSLAIIVGAGSWLLITGLVSRSIGRLPSSVIMSAVAVPYFGYP
                     VWAFWLGRHLLRW"
     gene            complement(1359..2255)
                     /locus_tag="AC16CB_14410"
     CDS             complement(1359..2255)
                     /locus_tag="AC16CB_14410"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3343711.1"
                     /translation="MNQTPDSKTYAAFVLTMLIGGANFIAVSLSNRELPPLFGATLRF
                     ALATFLFFLIARAWRIPLARGRDAAGAVIYGLLNVGAAYALLYYALVSLNAGTVAVIV
                     ASVPLFTLAIAVLFGQERLSFNRVAGGILAIAGIAILRGFGGDFVLSYFVAAVLGAVA
                     IAASSVVAKAYPNVHPINMNAIGTAAGTLLLAVSSLVFGEQWVLPHENTTWFAVGWLV
                     IAGSVGLFQLFLFVIKRWTASATVYALTGMPVIATALGIIILDQPVTMEVLAGGALVI
                     AAVYVGAIARQRKTSKFYRQKA"
     gene            complement(2471..3001)
                     /locus_tag="AC16CB_14415"
     CDS             complement(2471..3001)
                     /locus_tag="AC16CB_14415"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3343712.1"
                     /translation="MLKISQVESEEQIIAARELFREYTDWIFTLAVGHENAPTFQGLD
                     EELRTLPGIYSPPTGRLLLATQDGQPAGCVCLKGNDATTCELKRLYVRPSFRGQNIGW
                     QMVNLLVDEACQSGYQRIVLDSHIAMKKAHAIYQAVGFQIVEAPDDFPQYLRPTVVFM
                     ECDLSTNCERNGAGID"
BASE COUNT          884 a          852 c          787 g          753 t
ORIGIN      
        1 gatgcctgga gtccaccgga gcttcccaat ctgatgatat gcagccttgg aaaattagtc
       61 tttcgggttc gcgtctcgaa gtcaatctcg ttcaacgcca ccagctcgct caccacgatc
      121 tcaagggaag gtgtccccat ccctgaggtt gacacggtgg ttctcaccgg ggaaatgatc
      181 gtggtctgtt ctcctgtgat ttctgatgtt ccagttaatg taaccagccc tcggtgctca
      241 tgttttaact caaggtcacg cataagattg gaaccgatga attcagcacg cccgggatcc
      301 cctaccaaca ggatgtcagg ggcaatttgc ccgggtttga cctgaaggtg gtaaattctc
      361 cccccatcat caattggaag atcagacgca gtaaagggcg cagcagctct atgaagattt
      421 atcttgccag tattttgcgc cttctttata tttttctgct caggtctttc catcacatct
      481 tctccttact ataccagagg acatgacgta tcctaattta caccgcccaa agacaaagcg
      541 cagcagcagt tgcggctcca gtccgggacg attgtgaacc caaacctgtg catttcaagc
      601 cgttcgtttg acccagcggg gcagccgttt tttaccatct tagcaggtgt cgacctaacc
      661 agaatgccca gaccgggtag ccgaaatagg gtacagcgac agcactcata atcacgctac
      721 tgggtaacct tccaatcgat ctggacacca atccggtaat caacaaccag gaaccggcac
      781 cgacaataat cgccagagag acccaaaagc cctgttgctg gaaagtcaaa acttgaaata
      841 ttagcgccaa ctgcaaagcg ataatcaaca acatggccag aattcctaca attgttgcga
      901 cgagaatcag tgtcggactg tatggtcgta aaatacgata caaagccaaa gcaatcggga
      961 tagtcagcag gtattgaacg gatacacaga tgtcattgag taatccaaac gtgagagcct
     1021 gcgcctgata gggtgttgta aatagtataa acatggcaat caggaaaaca acgccgattg
     1081 cggaaatgat gccactgaga atggcaaagc cacccgcagc gcgaatcaca gggtcagttg
     1141 gcataatttg catagcgctg ttcctttggc agtatcgttg gtcttaggaa taccctaaga
     1201 ccactcaata gtatgcatta cctgctggag ggcgggttgg ttatctgcta gtatatcacc
     1261 ccggcagaga gaatagctac ccgggtacgc accgaaggtg gtcaggttaa aagctgtgtt
     1321 aatctgggtc ttactgtgct ttcaggattc gcatggcgct aagccttctg acggtagaac
     1381 ttcgatgttt tcctctgcct tgcgatggcg ccaacgtaga ccgctgcaat caccaatgcg
     1441 ccgcctgcca aaacctccat agtgaccggt tggtcgagta tgataatacc aagtgcagtg
     1501 gcaatcactg gcattcccgt aagagcatat acggttgccg aggcagtcca gcgtttgatc
     1561 acaaatagaa aaagctggaa aagacccacc gaaccagcaa tcacaagcca accaacagca
     1621 aaccatgtcg tgttttcatg cggaagcacc cattgctcgc caaatacgag cgagctgacg
     1681 gcgagcagga gcgttcctgc tgctgtgcca attgcattca tattgattgg atgcacatta
     1741 gggtatgcct ttgcgaccac gctggaagcc gcgatggcaa ccgcgccgag tacagctgca
     1801 acaaagtatg agagaacaaa atcgccccca aagccgcgca ggatcgctat accggcaatc
     1861 gcgaggatac cacctgcgac ccgattgaat gacaggcgct cctgaccaaa tagaacggca
     1921 atcgcaagcg tgaacagagg aactgatgcg acaataacag ccaccgtgcc ggcattgaga
     1981 ctgaccaggg cgtaatacaa gagtgcatac gcagcgccta cattaagaag cccatagatc
     2041 acagcccctg ctgcgtcccg accgcgtgcc aaggggatac gccaagcacg ggcaatcaaa
     2101 aagaaaagga aagtagccag ggcaaaacgc aaggttgcac cgaagagtgg cggcagttct
     2161 ctgttgctca ggctgactgc aataaagtta gcacctccaa taagcatagt gagaacgaat
     2221 gcggcgtagg ttttactgtc gggtgtttga ttcataatta cccatccttc cgatacaata
     2281 atgcaatacc gctataagaa tggaattcct gacctgccta atgctttgcg ttacctgctg
     2341 gcaggcggac gtggattctg cttgggagca ataataactc aaatccagaa aataccagca
     2401 agcaccgcat aatctctgcc gtccacacca cacgtggttg ggccgcactt cgaattgatt
     2461 agtccagtta ttaatctata cctgcaccat ttctctcaca gtttgtggat aagtcgcatt
     2521 ccatgaatac cacagttggc ctcaggtact gtggaaaatc atcgggagcc tccacgatct
     2581 gaaagcccac agcttgatag atcgcatgcg cttttttcat cgcgatatgg ctgtccagca
     2641 ctattcgttg atatccagat tggcatgcct catccaccaa caagttaacc atttgccagc
     2701 cgatgttttg tccccgaaag gaagggcgca catagagccg tttgagttcg caagttgtgg
     2761 cgtcattgcc tttgagacat acacaccctg ctggctgtcc gtcttgtgtg gcaagaagaa
     2821 gacgcccggt tggcggggaa tagataccgg gtaatgttct caattcctcg tctaaaccct
     2881 gaaatgtggg cgcattctcg tgaccaacgg cgagtgtaaa aatccaatcg gtatactcac
     2941 gaaaaagctc acgggctgca ataatttgct cttctgattc gacttgagat atttttaaca
     3001 ttaacggaat cctttccacc tataaaacaa caactcgaag agtcgcacaa cagaaaagct
     3061 cagccgccag ctgccaacct gttacaccac gagtgaaatc agctcgtgaa catcggcgcg
     3121 ctggctggtc ggctgtaatg agtggtttgc cagcttttca tctgtgagtc agctggccat
     3181 cttcaatcat tgggactatg tgctctcggg aggcctgcct agcctgcctc catcacccgc
     3241 ttcagaccgt tcaaatcggt gtcaaactgg tttaga
//
LOCUS       DBNGSX010000488         6218 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_325324, whole genome shotgun sequence.
ACCESSION   DBNGSX010000488 DBNGSX010000000
VERSION     DBNGSX010000488.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6218)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6218
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_325324"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="AC16CB_14420"
     CDS             complement(<1..233)
                     /locus_tag="AC16CB_14420"
                     /inference="COORDINATES: protein motif:HMM:NF012899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid kinase family protein"
                     /protein_id="HIR3343713.1"
                     /translation="MKDPLSQLVFLKLGGSLITDKTTPRSPRHEVINRLAAEIAAACG
                     RNPSLRIVLGNGAGSFAHVPAKRYGTRQGVRTP"
     gene            complement(245..1192)
                     /gene="mvk"
                     /locus_tag="AC16CB_14425"
     CDS             complement(245..1192)
                     /gene="mvk"
                     /locus_tag="AC16CB_14425"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HIR3343714.1"
                     /translation="MPAITASAPGKVILFGEHAVVYGRPAIAVPVNEVRARAMVNADP
                     RGVPGTMRIQAPAIGLDALLADLPDDQPLAAAVSSVLSTLGISRVPACTLRVTSTIPL
                     ASGLGSGAAVSVAILRALSAFLGQPRSDEQVSALAFEVEKLHHGTPSGIDNTVITYVR
                     PVYFVRGQPIQTLQVAQPFTIVIGDTGISSPTAIAVGDVRRLWGEKPAHYESLFDSAG
                     QIARQARQAIESGRPDELGALMDANHSLLQEMGVSSPELDRLVSAARTSGARGAKLSG
                     SGRGGNMIALASPETASLVANALSAAGAYRTIVTTVHSK"
     gene            complement(1272..2144)
                     /locus_tag="AC16CB_14430"
     CDS             complement(1272..2144)
                     /locus_tag="AC16CB_14430"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017687324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HIR3343715.1"
                     /translation="MSALFRALRPRQWTKNSLLFAALVFDRQLTLSHLPAILHTIAGF
                     FIFCLLSGAVYIINDIMDREADLNHPQKSLRPIASGELNLTIARTVAIGLIVVLLPIS
                     YMLSPLFAGIALIYFVSNLAYSKWLKHVPILDVLTISLGFVLRVGAGVSLIVVTRFSP
                     WLYVVTTLGSLYIGFGKRRAELVLLAEGANSHRKVLDGYTIPLLDQFITIVSGTTIIA
                     YSLYTFSAPNLPDNHIMMLTIPFVLYGIFRYLYLIQVKHSGGAPEDVLLSDRPLQATI
                     LLWGISVLIIFYAF"
     gene            2435..3313
                     /locus_tag="AC16CB_14435"
     CDS             2435..3313
                     /locus_tag="AC16CB_14435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3343716.1"
                     /translation="MTFWKYYHLAKNIPDALHVLTQAPGPARLISGGTDLLLDLQQGR
                     HSPVHTLVDVTHVPEMSIIEIRQDRLFVGAAVPLNSVVASPLVQRHAQALVEAAGLIG
                     GPQVRNTATLGGNVAHALPAGDGTIALLALEASAEVANQRGLRQVPMRELFLGPGRSA
                     LDPQNDMLVGFYLPLRSTGQGSAFKRVMRPQGVAIAILNSAVWVQREGERIAGIRIAM
                     GPAGPTPFRALAAEETLRMQLMDEESVSRAIETLLAEARFRTSPHRATLEYRREMANI
                     LLTDSLTLAWHRTFEK"
     gene            3375..6209
                     /locus_tag="AC16CB_14440"
     CDS             3375..6209
                     /locus_tag="AC16CB_14440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR3343717.1"
                     /translation="MNMVINGQRMTIEALPGEMLSDLLRERLGLIGTKIGCNEAECGA
                     CTVLVDDQPVLSCVFPAAKADGKNIFTIEGLASLYSIARSNRSGDGKEEDAQETLHPL
                     QEAFIQHGAVQCGFCIPGQIMTAYALLRAVPEPSEADIRYALKDTLCRCAGYPTIIRA
                     IQTAAYSVRTGEPLAPPPIPGLDEMRPHGVIGTLQPRPDSVEKVTGKAIFTDDLKFEG
                     MLHARVKRAGVPHAIVRRIDVSKARLLPGVVAILTADDIPGEHYHGLVIKDWPSLVGI
                     GERVRYVGDSLALVAAETREIATQALELIEVELEPQPVVSDPVQSRTPQAPSLHPNGN
                     LLKHIKVRKGDVEQGLAEADVILEHTFHTQMTEHAFMEPECSIARPLPDGRMEIYVGS
                     QIPYSDRNDIARALGWPEERVRVVGQLMGGGFGGKEDIAGQIHAALLVNATGRPVKLL
                     FDRHESLLVHPKRHPTQIHVKVGAKRDGRLTGVETELYGDTGAYASLGEKVMTRATTH
                     SSGPYEVPHARADCYAMYTNNPPAGAFRGFGVTQSAFAIESMMDMLAEMLHIEPVELR
                     WLNALRVGSVTNTGQTLRESVGLLECIDRVDAEMRRIGGSDPFAPRRVDGAPQLVRAW
                     GFAAAYKNTGLGGGAPDKAGAEVELFANGTLEVRTSSAELGQGLVTVLQMIVAEEFAI
                     SPSKVRVLVMDTDLTPDGGPTTASRQTYVTGNAARGAAQALRQAITTTLAEKYDQPPE
                     RIRFVEGLAQVNGHTLPLGNIVAGMRAEGREPRAMYEYWAPATKPLGQGGDMHFAFSF
                     ATQAAEVEVNTLTGEVQVLRVIAANDVGKAVNPLGLHGQVEGGVMMGLGNALTEHYIV
                     ENGQVFTDHLARSRMPSIMHTPEIVSVVVEHPAADGPYGAKGVGEIVSIPTSPAITNA
                     IYNAVGVRVDRLPVDQAEILKNIRRQKATG"
BASE COUNT         1343 a         1621 c         1912 g         1342 t
ORIGIN      
        1 tcgggtgtgc gaaccccctg acgagtgccg taacgcttgg caggaacgtg cgcaaacgag
       61 ccagcgccat tgcctagaac aatgcgcagg gatgggtttc gaccgcatgc cgcggcgatc
      121 tccgcagcca atcggttgat aacctcatgc cgtggagatc gcggcgtcgt tttatcggtg
      181 ataagcgaac cgccgagctt gagaaaaact agctgagaaa gtggatcctt cattgtttcc
      241 tattttactt ggagtgtact gtagtgacaa ttgttcgata tgcgcctgcc gccgacaagg
      301 cgttggcaac caaagaggct gtctcgggcg atgccaatgc gatcatatta cccccacgcc
      361 cgctgccaga gagtttggct cctcgagcac cgcttgtgcg cgccgcgctc actagcctgt
      421 ctagctcagg cgaggaaaca cccatctcct gaagtaggct gtggttggca tccatgagcg
      481 cgcctagttc atcgggtcgc ccgctttcga tggcttgccg tgcctggcga gcgatctgcc
      541 cagcggagtc gaacaggctt tcgtaatgtg ctggtttttc tccccacagg cgtcgcacgt
      601 cgccgaccgc aatagccgtt gggctggata tacccgtgtc cccaatcaca atggtaaacg
      661 gttgtgcaac ttgcaaggtc tgaataggtt ggccgcgcac aaaatatact ggcctgacgt
      721 aagtgatcac agtgttgtca attccagacg gtgtgccgtg atgcagtttc tcaacctcga
      781 aggccagtgc agagacttgc tcatcggatc ggggttggcc caagaacgcg gataaggcac
      841 gcaggattgc caccgataca gccgcccctg atcccagccc ggaggcaagc ggtatggtag
      901 aagtgacccg cagcgtacag gcgggaacac gggatatccc gagcgtggat aatacactgc
      961 tcacggcagc cgctagtggt tgatcgtctg gcaggtcggc gagaagcgcg tccagtccaa
     1021 tcgctggcgc ctggatgcgc atggtgccag gcacgcctcg cgggtcggcg tttaccattg
     1081 cacgggcacg cacctcattc acggggacgg cgatagccgg ccgaccgtac acgacggcgt
     1141 gttctccaaa caagataacc ttgccagggg cggaggcagt tattgcgggc atgaggatcg
     1201 tatgatgagg gaaactgggc gaggataaag gtttgaaggt ggtaagaagc gcttcgcgag
     1261 tttcctcgga cctaaaaggc gtaaaaaatg attaaaacgg agattcccca caggagaata
     1321 gtcgcctgca agggtcggtc ggagaggagc acatcctctg gcgctccacc gctgtgcttc
     1381 acctggatca agtagagata gcggaagatg ccatacagca cgaaggggat ggttaacatc
     1441 atgatgtggt tgtccggcaa attgggtgct gagaaggtgt acaggctgta agcaattatg
     1501 gtggttccgg acacaatggt gatgaattgg tctaagaggg ggatggtata gccgtccagg
     1561 actttgcggt gggaatttgc cccctccgcc agtaagacaa gctcggcgcg gcgcttgccg
     1621 aagccgatat agagtgaccc aagggtggtg accacgtaca gccagggcga gaaacgcgtt
     1681 accacgatca acgatacgcc agcaccaact cgcaagacga atcccaagga gatggtgaga
     1741 acatccagga tcgggacgtg tttgagccac ttggagtatg ccaggttgct tacgaagtaa
     1801 atcaaggcaa tgcctgcaaa caaaggcgag agcatgtacg agatcgggag taagacgacg
     1861 atcagtccga ttgctaccgt gcgggcgatc gttagattca gctccccaga agcgatgggt
     1921 cgcaggcttt tttggggatg gttcaggtcg gcctccctgt ccatgatgtc attgatgata
     1981 tagaccgccc cagataagag acagaagatg aaaaagcccg cgatcgtgtg caaaatggct
     2041 ggcagatggc ttagggtgag ctggcggtca aaaaccagtg cggcaaagag caggctattt
     2101 ttcgtccact ggcgcgggcg cagcgcgcgg aataaggctg acatggtctt ccaaaacatg
     2161 gggctatgat agtagggatt gtgttattcg tcaaccaaga attctgtttg gcgcgaggag
     2221 catcgatacc gtaatatcgg ttcgtgtggg gctgaatggc tgatttatca aggggactta
     2281 ttgttttatg gactcaattg ctcggttttc gtgttaaaat aagacctact ggatcggcgg
     2341 tcgctgcagg gtttgcctgc tgcggcgtcg ggtcagtgta tcccgtcgaa aagagccgcc
     2401 gttctgatct tggctgcaag ggaaacgccg atccatgact ttttggaagt attatcacct
     2461 cgccaaaaac atccccgatg cattgcacgt tcttacgcag gcacctggtc ccgcccgact
     2521 gatctcgggt ggcactgacc tgctgttgga tttacagcag gggcgccact cccccgtaca
     2581 cacactggtt gacgttacgc atgtccctga gatgtcgatt atagagattc gccaggatcg
     2641 gcttttcgtg ggagcggctg tgcctcttaa tagcgttgtg gcatcacctc tcgttcagcg
     2701 acatgcccag gcgttggttg aggctgctgg actgatcggc ggaccgcaag tgcgcaatac
     2761 cgctactttg ggtgggaatg tggcacatgc gctgccggcg ggcgatggca ctatcgcctt
     2821 gctggccctg gaggcatcgg cggaggtagc caatcagcgg ggcttgcgcc aggtacctat
     2881 gcgagagtta tttctaggac ccggacgttc ggctctcgat ccgcaaaatg atatgctggt
     2941 cggattttat ttacccctga gaagcaccgg tcaaggatcg gcattcaagc gggtgatgcg
     3001 gcctcagggt gtcgcaattg ctatcctgaa ctcagcggtc tgggtacagc gagaagggga
     3061 acgcatcgcc ggtatccgca ttgcgatggg tcctgctggc cctactccat ttcgagcgtt
     3121 agccgctgaa gagaccctgc gtatgcaact tatggacgaa gagtcggtaa gccgggctat
     3181 tgagaccttg ctagctgagg cgcgcttccg caccagtccg caccgcgcca cgttggagta
     3241 ccggcgggag atggcgaata ttttgctgac agactcactt acgctggcat ggcatcgtac
     3301 ctttgaaaag tgaattcgga agatagacaa cagaaagatt ggatagttta ttaggtgagc
     3361 aggaagagtt tgttatgaat atggtgatca atggacagag aatgacgatt gaggcgctgc
     3421 ctggtgagat gctcagcgat ttgctgcgcg agcggcttgg gttaatcggc acgaagattg
     3481 gttgtaatga ggcggagtgt ggagcctgca ccgtcctggt ggacgatcag ccggtgctct
     3541 cctgtgtttt ccctgctgcc aaagcggatg gcaaaaatat ttttaccatc gaaggtttgg
     3601 cttcacttta ttctattgct cgttccaatc gtagcggaga tggaaaagag gaggatgctc
     3661 aggagactct ccaccccctc caggaagcct tcattcagca tggcgcggtg cagtgcggct
     3721 tctgcatccc tgggcagatc atgacagcct atgccttgtt gcgagccgtg cccgaaccta
     3781 gcgaggcaga cattcgctat gccctgaaag atactttgtg ccgctgtgca gggtacccga
     3841 cgattatcag agctatccag acagctgcct attctgtgcg tactggggag ccacttgcgc
     3901 ccccgcctat cccgggtctt gatgaaatgc gcccgcatgg agtgattggc acgctccagc
     3961 cacgtcctga ctcggttgag aaagtgactg gtaaagctat attcaccgac gatctaaaat
     4021 ttgagggtat gctccacgcg cgcgtcaagc gcgctggggt gccccatgca attgtccgcc
     4081 gtatagacgt ctcgaaagcc aggctattgc ccggtgtggt cgccatcctg acggcagacg
     4141 atatccccgg cgagcattat cacggactgg ttatcaagga ttggccaagc ctggtgggga
     4201 taggagagcg ggtgcgttac gtgggagatt ccctagctct ggtcgctgca gagacgcgtg
     4261 aaattgcgac tcaggcacta gaactcatcg aagtagaact tgaaccgcag cctgttgtca
     4321 gtgatcctgt tcaatcgcgc acgccacaag cgccgagttt acatccgaat ggcaatctgc
     4381 tcaaacatat caaggtgcgc aaaggtgatg tcgaacaggg tttggctgag gccgatgtga
     4441 tcctggagca cacgtttcac actcaaatga ccgagcatgc cttcatggag ccggagtgca
     4501 gcatcgcgcg tcctttgcct gacgggcgca tggagatcta tgttggctca cagataccat
     4561 attcggatcg caatgatatt gcccgcgcgc tgggctggcc tgaggagcgg gtgcgcgtgg
     4621 tgggccagtt gatgggagga ggttttggcg gcaaggagga tattgccggt cagatccacg
     4681 ctgcgctgct ggtgaatgcc acgggtcgcc cggtgaaact actattcgac cggcatgaaa
     4741 gcctgctggt gcacccaaaa cggcacccca cgcagatcca tgtcaaggtc ggggcgaagc
     4801 gtgatgggcg cctgacaggg gttgagactg agctttatgg ggatactggc gcctatgcgt
     4861 ccttgggtga gaaagtcatg acccgcgcca ccacccattc gtctggccct tacgaggtgc
     4921 cccatgcccg tgcggattgc tacgccatgt acacaaataa cccccctgcc ggtgcattcc
     4981 gcggcttcgg tgtgacccaa tcggcgtttg caattgagag catgatggat atgcttgccg
     5041 agatgctgca cattgaaccg gttgaactac gctggctgaa cgccctgcgt gtgggcagcg
     5101 tgaccaatac tggtcagacc ttgcgcgaga gtgtagggct attagaatgc atcgaccgcg
     5161 tggatgcgga gatgcgtcgt attggcggga gcgatccatt tgccccgcgc cgcgtggatg
     5221 gtgcgcctca actggtgcgt gcttggggat tcgctgcggc atacaagaac acaggattgg
     5281 ggggtggcgc gcccgataag gctggcgcag aagttgaatt gttcgccaac ggcacgctgg
     5341 aggtgcgtac ttcttctgct gagcttggtc aggggctggt caccgtgctg cagatgatcg
     5401 ttgctgagga gttcgctata tcaccttcga aggtgcgtgt gctggtcatg gatactgatc
     5461 tgacccccga cggtggcccg acgaccgctt cccgccagac ttatgttaca gggaatgccg
     5521 cccgcggcgc cgcccaggcg ctccgacagg ctatcaccac caccctggcg gagaagtatg
     5581 atcagccccc agagcgcatt cgcttcgtcg agggattggc ccaggtaaac gggcacaccc
     5641 tgcctctggg caatatcgtc gccggtatgc gcgccgaagg ccgcgagccg cgcgccatgt
     5701 acgaatactg ggcaccggct accaaaccgc ttggtcaagg cggcgatatg cacttcgcct
     5761 tttcctttgc aacccaggct gccgaagtgg aggtgaatac gctgactggc gaagttcagg
     5821 tgctgcgcgt aattgccgcc aacgatgtag gcaaggctgt caatccgctg ggcttgcacg
     5881 ggcaggttga aggaggcgtg atgatgggct tgggcaacgc gctcactgag cactatatcg
     5941 ttgaaaatgg gcaggtgttt accgaccatc tggcgcgctc tcgtatgccg tccatcatgc
     6001 acacgccgga gattgtctcc gtcgtcgtgg agcaccctgc agcagatggc ccatatggag
     6061 ccaaaggggt cggggaaatt gtcagcattc caacctcgcc ggcgatcaca aacgccattt
     6121 acaacgcggt tggcgtgcgc gtggaccggc tgcctgtaga tcaggcagag attctaaaga
     6181 acattcgccg gcagaaggct accggttgat gcctaacc
//
LOCUS       DBNGSX010000489         8348 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_325616, whole genome shotgun sequence.
ACCESSION   DBNGSX010000489 DBNGSX010000000
VERSION     DBNGSX010000489.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8348)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8348)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8348
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_325616"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="AC16CB_14445"
     CDS             complement(<1..722)
                     /locus_tag="AC16CB_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluzincin family metallopeptidase"
                     /protein_id="HIR3343718.1"
                     /translation="MMQPPLPSTPNEFISWSWGQIEPYYQELADRQLNSHTLQAWLTD
                     WSNLSKMLYEMFWRLNVAVTVNTADHEAERRYIAFLDEIQPPSQAAEQKLKQKLLASS
                     LSPDDFEIPLRNIQAEAQLFREENLPLLSEELKLGSEYDKIVGAQTVTWQGDEITLAQ
                     LAPVYRDKDRALREQAWRLAHSRWLEDREAINTVWGKLLSLRRQLAANAGLPDYRAYR
                     WRKLLRFDYTPEDCLKFHQAIE"
     gene            complement(747..1856)
                     /locus_tag="AC16CB_14450"
     CDS             complement(747..1856)
                     /locus_tag="AC16CB_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343719.1"
                     /translation="MKSGQRNAIIVLSAIAALSLTFAIVIFLLLYPGWAKRSPLPITP
                     TSVQVEMLENTLPADTSPTLDTGSSPLDGTYTKVDPSWPQWWLCRRCADYRLAGGIWK
                     LVFDKGMMHIYYDITGWRSMASFNISGDRLHIFNDPYCPDDEGEYTWEQGGGQIKLEV
                     IDDTCSFQLRGKNLSNQPWLTCLPPEKTTHPASDWQAPPGCEGSPTMPEPAPASNLPI
                     NVTVHGGDSRFFKKPPDVFANANNADIPSPEGIHVTYHDQSIPYGLSRVLWWEGDWIQ
                     ASTELPFASMGVQILGDPPIGWARLLFDGVEVWRGKTSAIWSYHGRHGGYVEISGFSP
                     GTHVIRAESMAFDYRPVTVASFGFSYHEGVKSQEP"
     gene            complement(1894..3369)
                     /gene="gatA"
                     /locus_tag="AC16CB_14455"
     CDS             complement(1894..3369)
                     /gene="gatA"
                     /locus_tag="AC16CB_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIR3343720.1"
                     /translation="MPELTDLTLTEAIAALRAGDTTSHELTTAYLERIQRLDSSLHTY
                     LTLTPELALQQAGEADRHISAWRKAPGPLAPLMGVPLAVKDVLCVAGVRTTAGSRILE
                     NFVPPFNATAVERLRAAGVVILGKTNTDEFAMGSSTENSAYGVTRNPWNPEHVPGGSS
                     GGSAAAVAARLAPAALGTDTGGSVRQPASFCGVTGLKPTYGRVSRYGLIAYGSSLDVV
                     GVFGHSAADLAPIFTQMAGYDARDATTVEQPVPQISLNNQVDLEGLRVGVPDEYFISG
                     IQPEVEAAVRQAVSVLESLGAQARPVSLPHTRYALPVYYLIAPAEASANLARYDGIRY
                     GPRLPADTMWDVFRHTRGEKFGAEVKRRIMLGTYALSAGYYDAYYGQAQKVRTLIKGD
                     FERTLQDVDVIAAPVAPTTAFKIGGHGDDPLSMYLEDVFTLPANLAGVPGLTFPVGFD
                     GMGLPIGMQLMGAHFGEDVLFKVAHAYQQVTSWHQAIPALS"
     gene            complement(3446..3733)
                     /gene="gatC"
                     /locus_tag="AC16CB_14460"
     CDS             complement(3446..3733)
                     /gene="gatC"
                     /locus_tag="AC16CB_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005551236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR3343721.1"
                     /translation="MPLTLKEVEHIANLARLDLTDDEKARYREQLSAILDYFTQLSEL
                     DTGGIPPTSSVLSPRSVLRDDEPHPGLSLEDLLRNAPDAEAGQFRVPFILE"
     gene            complement(3801..4748)
                     /locus_tag="AC16CB_14465"
     CDS             complement(3801..4748)
                     /locus_tag="AC16CB_14465"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012958.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3343722.1"
                     /translation="MLTLAILLCLGLASCAAKDPSLGTPLSTSDIAPSPLPTKTSTPT
                     ITPSPQPSNTPSPLPPTLTPIPSLTPSPTPLACWGEPGNKEHASLSSDLLRLPLEYTV
                     YLPPCYDQQKDRRYPVLYLIHGQSYNQDQWDRLGADETADALIAAGEVPPFIIVLPRD
                     RSWAQPTEDMFGQVVAEELVPTIDEGYRTLSERAYRAIGGLSRGAGWAVHLGLSRWEL
                     FGAIGGHSLPVFWSDTPYIRRWLKDIPDDALPRIYLDIGEKDRPNILDSAIWFEKLLT
                     TEGIPHEWHLYAGYHEEAYWESHVDEYLRWYADGWMGSE"
     gene            complement(5005..5265)
                     /locus_tag="AC16CB_14470"
     CDS             complement(5005..5265)
                     /locus_tag="AC16CB_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HIR3343723.1"
                     /translation="MDIIKVSGRSRTSAVAGAIAGVFRENKRVEVQAIGASAINQAVK
                     AIALAKSYLAEDGYSIVCIPEFVDVEIEEKIRTAIKFVIEQR"
     gene            5580..6704
                     /locus_tag="AC16CB_14475"
     CDS             5580..6704
                     /locus_tag="AC16CB_14475"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HIR3343724.1"
                     /translation="MDIQTVSDLDTPPRILLGPGPSMVSPRVLRAMAQPLLGHLDPQF
                     LALMDEVQQLLRYVFQTQNELTIPVSGTGSAGMEAALCNFIEPGDRVLVAINGYFGER
                     MVDMAGRYGAQVDRLECPWGQVFDPGDIEAALRGKQYKLVALVHAETSTGAHQTGIAE
                     IAAAAHRHGALLVLDTVTSLGGVPVEIDAWEVDVAYSGTQKNLSCPPGLAPLTVSPRA
                     RDVLRGRKTPVANWYLDLSMLDKYWGHDRTYHHTAPISMNYALREALRIVAEEGLEPR
                     FARHRLHAEMLWDGLEALDLPPFVAIEYRLPTLTTATLMPSIDDAAVRRRLLDEYNIE
                     ISGGFGPLKGKVWRIGLMGFSSRRENVTLLLAALQHILSG"
     gene            6718..7074
                     /locus_tag="AC16CB_14480"
     CDS             6718..7074
                     /locus_tag="AC16CB_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343725.1"
                     /translation="MSRWIRFFLAIALGAAGGLFYGWVVSPIQYIDTTPDTLRIDYKS
                     DYILMVAETYSVDKEMALAARHLGLLGDTAPVEMVREAVLFAESQGYTDADVALMRQL
                     LTDLQSWIPSLETPSP"
     gene            7071..7952
                     /locus_tag="AC16CB_14485"
     CDS             7071..7952
                     /locus_tag="AC16CB_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343726.1"
                     /translation="MSDERGPWYLITGLVLGAVMGVLYAWVLQPVQYTDTTPASLRAD
                     FKDQYRAMIALAYMANGDLVRAGARLDLLEDEDVYKTLAEQAQRTLAGGSASQEARAL
                     GMLAVALGQSPSTLAAPGGGEPGSVPQETQVITPTLSFSAVLTRTLPAATEESTAQPT
                     ETSPTDSPTITPLPTRQPTPTIGSPFVLKSEELSCNPAQETPLIIVQAYDAAGQGIWG
                     VEAVVNWEDGESHFFTGLKPELGIGYADFTMTPGVVYTLRLAEGGQTIPNLTPKECEA
                     EGGERYWGSWLLVFAQP"
     gene            8007..8267
                     /locus_tag="AC16CB_14490"
     CDS             8007..8267
                     /locus_tag="AC16CB_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343727.1"
                     /translation="MMRIVLDRRSCQCYDQACESHFGWHFLRDDITPVDCTLEMVDDQ
                     RAETTFLILDKDGIDKELVVNDENWGIAYDSWRQAWEQQQAK"
BASE COUNT         1761 a         2265 c         2483 g         1839 t
ORIGIN      
        1 tgttcgatag cctggtggaa tttcaggcaa tcctcgggcg tgtaatcgaa gcgcaacaac
       61 ttccgccagc ggtaggcgcg gtaatccggc agcccggcgt tggctgccag ctggcggcga
      121 aggctgagca gcttgcccca gacggtattg atcgcctccc gatcttccag ccagcggctg
      181 tgagccagtc gccaggcttg ctcgcgcagc gcgcgatctt tgtctcggta aactggtgcc
      241 agttgtgcca gggtgatttc gtcaccttgc caggtgacgg tctgcgcgcc aacgatctta
      301 tcgtactctg agcctagctt gagctcctcg ctcaataggg gcaggttctc ttcgcggaaa
      361 agctgcgctt cggcctgaat gttacgcagg gggatctcaa agtcatccgg gctcagggag
      421 ctggccagca gcttttgctt caacttctgt tcggcggcct gcgaaggggg ctggatctcg
      481 tccaggaagg cgatatagcg cctctcggct tcgtgatcgg cagtgttcac cgtcactgcc
      541 acgttgagcc gccagaacat ttcataaagc atcttggaga ggttcgacca gtccgtcaac
      601 caggcttgca gagtgtgcga gttaagctgg cggtcagcca attcctggta gtagggctcg
      661 atctgccccc aggaccagga tataaattcg ttaggggtgc ttggaagcgg aggttgcatc
      721 atctttcctt ccttataaaa tatgttctaa ggttcttggc tcttgacacc ttcatgataa
      781 ctgaacccaa aacttgccac ggttacagga cggtagtcaa aagccatact ctccgcacga
      841 atgacgtgcg ttccggggct gaatccggag atttctacgt acccaccatg gcgaccgtga
      901 taggaccaaa tcgcggaagt ttttccgcgc catacttcaa caccgtcgaa aaggaggcgt
      961 gcccagccaa tcggagggtc acccaggatt tgcactccca tagaagcgaa gggcagttcc
     1021 gtggaggcct ggatccaatc accttcccac cacaggacgc gactcaagcc atatgggata
     1081 ctctgatcat ggtaagttac atgaatgcct tctggtgaag ggatatcggc attgtttgca
     1141 ttggcaaaga catccggcgg tttcttaaaa aaacggctgt cgccaccatg gacagtaaca
     1201 ttgattggta gatttgatgc aggtgcaggc tcgggcatgg ttgggctgcc ctcacaaccg
     1261 ggaggtgcct gccagtcgct ggcaggatgg gttgtcttct ctggcggcag acaggtgagc
     1321 caaggctggt tgctcagatt cttgcctctc agttgaaaag agcaggtgtc atcgattact
     1381 tccagcttga tttgcccgcc accctgttcc catgtgtact ctccctcatc gtctggacag
     1441 taaggatcat tgaagatgtg cagacgatca ccagagatat taaatgaagc catactccgc
     1501 cagccggtta tatcgtaata gatgtgcatc atgcctttat caaataccag tttccaaata
     1561 ccccccgcga ggcggtagtc cgcgcagcgc cggcacagcc accactgggg ccaagacgga
     1621 tcgaccttgg tataggtccc atctaagggg ctagagccgg tatcaagagt aggggatgta
     1681 tctgcgggaa gggtattctc caacatttca acctggacgc tggttggggt gattggcaga
     1741 ggggatcttt tcgcccatcc aggatagagt aacaggaata ttacaatggc gaaggtgagg
     1801 ctgagcgcag ctattgcgga tagaacaatg atcgcgttgc gttgcccaga tttcatgatc
     1861 ccgtgctcag aacggataaa tgcatcatgg tccttagctt aaagctggga tcgcctgatg
     1921 ccagctcgta acctgctggt aggcatgggc cactttgaac aaaacatctt cgccgaagtg
     1981 cgcccccatc agctgcatgc cgataggcaa tcccatgcca tcgaagccca ccgggaaagt
     2041 cagcccgggc acgcccgcca ggttggcggg cagggtgaac acatcttcaa gatacatgga
     2101 gagagggtca tcaccgtgcc caccaatctt aaaagcggtg gttggggcga caggcgctgc
     2161 aatcacatcg acatcctgaa gggtgcgctc gaagtcaccc ttgattaagg tacgaacctt
     2221 ttgtgcctgg ccgtaatagg cgtcgtaata gccagcggag agggcgtatg tacctaacat
     2281 gattcggcgc ttgacctccg cgccgaattt ctcgccacgc gtgtggcgga acacatccca
     2341 catggtgtcg gcaggcaggc gaggcccata gcgtatgccg tcgtaacgcg ctaggttagc
     2401 ggaagcctct gcgggggcaa tcaggtagta gaccggcaat gcgtagcggg tatggggcag
     2461 actcactggc ctggcctggg cgcccaggga ttccagcacg cttacggcct ggcgcacagc
     2521 cgcctccacc tcgggctgta taccggagat gaagtattca tcagggacgc ccacgcgcaa
     2581 gccctccaga tccacctgat tattcaatga tatttgcggg acgggctgtt ctacggttgt
     2641 ggcatcgcga gcgtcataac cagccatctg ggtgaaaatt ggagccagat cggcggcact
     2701 gtgcccaaat accccaacga catccaggga agaaccgtag gcgatcagcc catagcgcga
     2761 gacccgccca taagtgggtt ttaagccggt cacgccgcag aaggaggctg gctggcgcac
     2821 gctgccgcca gtatcggtgc ctagggctgc aggagccagg cgcgccgcca cggctgccgc
     2881 gctgcccccg ctagagccac cagggacatg ctcgggattc caggggttac gggttacgcc
     2941 ataggccgag ttctcagtgg aagagcccat ggcgaactca tcggtattgg ttttgcccaa
     3001 tatcaccacg cccgcagccc gcaggcgctc caccgcggtg gcgttgaagg gaggcacaaa
     3061 gttctccagg atgcgcgaac cggcggtagt ccgcacgccg gcaacgcaca acacatcctt
     3121 taccgccagc ggcacgccca tgagtggcgc caggggaccg ggcgctttgc gccaggcgct
     3181 tatgtgtcga tcggcttcac ctgcctgctg cagtgccagc tcgggggtca gggtgagata
     3241 agtgtgcagg ctggagtcca agcgttggat acgctccagg taggctgtcg tcagctcgtg
     3301 actggtagta tccccggcgc gcagggcggc tatggcttca gtgagcgtca gatccgtcaa
     3361 ctcgggcatg ggatactcaa attcgggtgc gtgtggaatg gaataaacag gtatttcctg
     3421 ttttcccgct caaatctcgg ctaattcact ccaaaataaa cgggacgcgg aactggcctg
     3481 cctcggcatc aggcgcgttg cgcagtaaat cttccaggct cagaccgggg tgcggctcgt
     3541 catcgcgcag gacgctgcgg ggtgataaca cgctggaggt aggtggtatg ccgcctgtgt
     3601 ccagctcact cagttgggtg aaataatcca ggatggctga gagctgctcg cggtagcgag
     3661 ccttttcatc gtccgtcaga tccaggcgag ccaggttggc gatatgttcg acttccttaa
     3721 gagtgagggg cataacaggg attatatcat tcaggtcatc gattgcaggg ctgtgattgg
     3781 cacctggtaa cttgctgtgc ttactctgaa cccatccagc catctgcgta ccagcgcaaa
     3841 tactcatcta cgtgcgattc ccagtaggct tcctcgtgat atccagcgta caggtgccac
     3901 tcgtggggga tgccctctgt tgtgagcagt ttctcaaacc agatggctga gtcgagaatg
     3961 ttcggtcgat ccttttcgcc gatatccaga tagatgcgcg gcaaggcatc atccgggata
     4021 tccttcagcc agcgacggat atagggcgtg tcactccaga ataccggcag gctatgcccg
     4081 ccgatcgccc cgaagagttc ccagcgcgag agccccaggt gcaccgccca acccgcaccg
     4141 cgcgataagc ctccaatggc gcggtaagct cgctctgaga gcgtgcggta accctcgtca
     4201 atggtgggga ctagctcttc tgccacaacc tgcccaaaca tatcttcggt gggttgcgcc
     4261 caggagcggt ctcggggtaa tacaatgatg aaggggggca cttcgcccgc agcgatcagg
     4321 gcgtcggcgg tctcatctgc gcctagacga tcccactggt cttggttata actctgtccg
     4381 tggatcaggt acagcacggg atagcgacga tccttctgct gatcgtagca aggaggtagg
     4441 tacaccgtgt actctagcgg caggcgcagc aggtcgctgg ataggctggc gtgctctttg
     4501 ttgccgggct ccccccagca tgccagcggg gtgggagagg gcgtcaggga tgggatgggt
     4561 gtgagggttg ggggcagcgg cgagggggtg ttggaaggct ggggagaggg cgtgatggtg
     4621 ggtgtgctgg tttttgtggg taggggagat ggggcgatgt ctgatgtcga taagggtgtg
     4681 ccaagggatg ggtctttggc tgcacaactt gccagtccca ggcagagcag gatcgcaagc
     4741 gttaacaaag agaccctgat gtgataattc cccccaccgc ttccagcgga gtggcgggga
     4801 agtagggttg gatgatgaga ttgggtaatt agtttttttg atattgggga tatcgccatc
     4861 ccgccaagtg tatcaaaaga taaacagccc gtccagaaac gaaagggcca cgcagtgaat
     4921 atatgcgtgg cacaacaacg gcagttctga tttcacagaa tgctttgctt tggcgaccgc
     4981 ctgggtcgat tgatcggtgg tattttagcg ctgttcgatg acaaacttaa tggcggtccg
     5041 aattttctcc tcgatctcca cgtccacaaa ttctgggatg catacaatgc tatagccgtc
     5101 ttctgctaga taactcttcg ccaatgctat cgctttgaca gcctgattaa tagcgctggc
     5161 tccaatggct tgcacttcga cacgcttatt ctccctaaaa actcctgcga tagcccccgc
     5221 cacagcggaa gtgcgtgacc tgcctgacac tttgatgatg tccatgccgc ttcctccccg
     5281 aaaccattgt tcgctgtgtt gtggccgctg tgagacgacc taacgctttc attattattg
     5341 tacaagatat ttatgcagtt ttcaataaga tgattcgcaa attttctcag aattgatagc
     5401 cctggcaatt aaaaccctgg cagatgaaat ttcaattctt ggtgaagttc gatttttgtt
     5461 gtaaactatt gttattacgc ggttcgacaa ccgtaaggtt acaattgagc atagttactt
     5521 ttgctctgag agcagcggtg accttctttc atatcatctg atctatcaaa aggaatttca
     5581 tggatataca aactgtctcc gacctggata cccctccccg aatattactc gggccgggtc
     5641 ccagcatggt ctcgccgcgt gtcctgcgcg ccatggctca gcccttgctg ggtcacctgg
     5701 acccacaatt tctggccttg atggatgagg tgcagcaact gctgcgctat gttttccaga
     5761 cgcaaaacga gctcaccatt ccggtctctg gcactggcag cgcaggcatg gaagctgcct
     5821 tatgcaattt tatcgaacct ggggatcgcg tcttggtcgc catcaacggg tactttggcg
     5881 aacgcatggt cgatatggct gggcgttatg gagcccaggt ggaccgcctg gagtgcccct
     5941 gggggcaggt ctttgaccct ggggatattg aggcagccct gcgaggaaaa cagtataaac
     6001 tggtcgccct ggtgcacgct gaaacctcta caggggccca ccaaacgggc atcgctgaga
     6061 tcgctgcagc cgctcaccgc cacggggcgc tgctggtgct ggatacggtc acctctctgg
     6121 ggggcgtgcc agtcgagatc gatgcctggg aagtggatgt ggcttacagc ggcacgcaga
     6181 aaaacctgtc ttgcccaccc ggcctggctc cattgactgt ctcgccacgc gctcgcgatg
     6241 tgctacgcgg gcgcaagacc ccggtggcaa actggtatct ggatctttca atgctggaca
     6301 aatactgggg gcacgaccgc acctatcacc acaccgcccc aatcagcatg aactatgccc
     6361 tgcgcgaggc gctgcgcata gtggctgaag aggggctgga accgcgcttt gctcgccatc
     6421 gcctgcacgc agaaatgctt tgggatgggc tggaagccct ggacttgccg ccctttgtgg
     6481 ctatagagta ccgcctgcct acgttgacta ccgctactct gatgccatct attgatgatg
     6541 ccgccgttcg ccgccggctc ctggatgagt acaacatcga aatctcaggg ggctttggac
     6601 ccctgaaagg caaggtgtgg cgcatcggac tgatgggctt cagcagccgg cgcgagaacg
     6661 tgacactgct gttggcggcc ttgcagcaca tactgtctgg ctaaggcagc atcgacgatg
     6721 tctcgctgga tccgattttt tctggcaatt gctcttggcg cagcgggggg gctattctat
     6781 ggctgggtag tcagcccaat tcagtacata gataccaccc ctgacacact gcgcatcgat
     6841 tacaagagcg attacatcct gatggtggca gagacctaca gcgtcgataa ggagatggcg
     6901 ctggctgccc gccacttggg attgctggga gatacagcgc cggtcgagat ggtgcgtgaa
     6961 gcggtgctct ttgcagaaag ccagggctac accgacgcgg atgtggcctt gatgcgccag
     7021 ctcctgaccg atcttcagtc ctggattccg tccctggaga caccttcgcc atgagcgatg
     7081 agcgcggtcc ctggtacctg atcaccggct tggtgctcgg tgcagtgatg ggagtgcttt
     7141 atgcatgggt tttgcagcca gtccagtata ctgacactac ccccgcctcg ttgcgcgcag
     7201 acttcaaaga ccagtacagg gctatgatcg ccttggcata catggctaat ggcgacctgg
     7261 tgcgggctgg ggcgcgcttg gacctgctcg aagatgagga tgtgtacaag acgctggctg
     7321 agcaagccca gcgcaccctg gcgggtggca gcgcctcgca ggaggcgcgc gctctgggta
     7381 tgctggcagt tgccttgggg cagtcgccat ccactttggc cgcgcctgga gggggtgaac
     7441 ctggctcggt ccctcaggag acacaggtga tcacccccac cctgtctttt tcggctgttc
     7501 tgaccaggac acttccggcg gcaactgagg agagcacagc ccagccgacc gaaaccagcc
     7561 ccacggattc gcccaccatc acgcccctgc ctacgcgcca gccgacaccg acaattgggt
     7621 cgccctttgt cctgaaaagc gaggagctgt cctgcaaccc tgcccaagaa acaccgctga
     7681 tcatcgtgca ggcgtatgac gccgctggtc agggcatctg gggagtggaa gccgtggtaa
     7741 attgggaaga tggcgaaagt catttcttca ccggcctgaa gcccgaactg ggtatagggt
     7801 atgcagattt caccatgacc ccgggtgtag tctacaccct gcgcctggca gaaggcgggc
     7861 aaaccatccc caacttgacc cccaaggaat gcgaggctga aggtggggag cgctattggg
     7921 gttcctggct gctggttttc gcacagccgt aattgatatg aatactgagg gatagataaa
     7981 agcatcttac gaaaatttag gaggttatga tgagaattgt tttggaccga cgctcttgcc
     8041 agtgttatga ccaggcatgc gagtctcatt ttggctggca tttcctgcgg gatgacatca
     8101 caccggtgga ctgcaccctg gagatggtgg atgaccagcg ggcggagacc acttttctga
     8161 tcctggataa ggatggtata gacaaggaac tggtcgtgaa tgacgagaat tgggggattg
     8221 cctacgattc ctggcgacag gcctgggagc agcaacaggc gaagtaagat gaaataatgg
     8281 cttacctgta cggcgcgtgc ctgatcctcg ggctgatggt gaccctgtgg ctgctcagcc
     8341 tgtggctg
//
LOCUS       DBNGSX010000490         9781 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_118328, whole genome shotgun sequence.
ACCESSION   DBNGSX010000490 DBNGSX010000000
VERSION     DBNGSX010000490.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9781)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9781)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9781
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_118328"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="AC16CB_14495"
     CDS             complement(<1..617)
                     /locus_tag="AC16CB_14495"
                     /inference="COORDINATES: protein motif:HMM:NF014420.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIR3343728.1"
                     /translation="MLVAWKYRPRDTFIQRLDPRARLTFLACIILAVSGAQVWDIRLL
                     LPLFILTMVLYLMARIEWRDVWRAWAFILFLVIFIVGINGLLAGRGGPTAVLQDQSAA
                     LYKSPTLHIPFTPWSITLTITVVRSFFAITQIVRMLTMAILAIPIPYTMDPSIYGVTF
                     RRMGLPDKASFTMDLAFRFIPTLGRDFMITVDAQRARGYELESAKG"
     gene            complement(618..1169)
                     /locus_tag="AC16CB_14500"
     CDS             complement(618..1169)
                     /locus_tag="AC16CB_14500"
                     /inference="COORDINATES: protein motif:HMM:NF018816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR3343729.1"
                     /translation="MDNQSGLWSFSTRQVVYGAIGAALYGVFSWITNVIPLPAAGNVT
                     FRPAVAVLIFFGIAYGPWVGLIAGLVGNTLGDGLSGWGFFWNWSVGNGLMGMIPGLAM
                     AMIQDYRSGRSIGIGIGWGALGIAVGMLFASLTEMFVGGIDLSTALVGYFTPAFIGNL
                     VVTAVLLPILMVAFAGVTSRRGR"
     gene            complement(1301..2947)
                     /locus_tag="AC16CB_14505"
     CDS             complement(1301..2947)
                     /locus_tag="AC16CB_14505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3343730.1"
                     /translation="MSEEYPLVVENLSFRYHSRPELAIQDVSFQLRRGELLLVAGASG
                     CGKTTLARCINGLIPRSYRGDRAGSVFIHGKEVSAMPLVEVAQVIGTLLQDPERQIVA
                     SNVWNEIAFGPENLGLSTQEIGERIDQVIARLNIEHLRGRDTFTLSGGEKQKVALAGL
                     LVMRPSILLLDEPLASLDPASAHEALAIFRSLADEGSTILLVEHRVEDALAARPDRLL
                     YMSAGRVRYLGAVDSLPPEIDHREVKLPAPWVVQRVHQTQPTQSALQPPPQAVAEGAP
                     LIEFKDVSFAYGDGPEVLHEVNLKIHQGDLIAVLGPNGAGKSTLVKHAIGLLKPTRGL
                     VQVEGVDTRKMTVAQTARKVGYVFQSPTHMLFAPTVREELAFGPANLGYSRDEIEQNV
                     VASVAAVNLTGLEEYPPLGLSFGQQKRTGIASVMAMRSMIMVLDEPTAGQDYANYTQF
                     MEILCGESGEDGKSLLTANFAATLFITHDLDLAVTYANRVILVGEGRVVADGPPEQVL
                     RDFELLQRYRVRPTSLLRMNLDLLSKTGRFLPAEALAVYA"
     gene            complement(2950..3744)
                     /locus_tag="AC16CB_14510"
     CDS             complement(2950..3744)
                     /locus_tag="AC16CB_14510"
                     /inference="COORDINATES: protein motif:HMM:NF014003.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM hydrolase/SAM-dependent halogenase family
                     protein"
                     /protein_id="HIR3343731.1"
                     /translation="MSIISLMTDFGIKDGNVGVMKGVIWNISPDAQIADLSHTISPQN
                     VLEASLILARSAPYFPAGSIHLVVVDPGVGTNRRHIAARLGMQYFVGPDNGVISGLLE
                     YAEAEDWTVEIVHTDKRQYWLPKVSHVFHGRDVFAPIAAHLARGVPLYALGTPIHDPV
                     RLSLPKPQTTPSGWQGEVIHIDHFGNISTSIRVENLGYPDHVKVGLRDVIITGLVRTF
                     GERTPGELVALYGSTGNLIISVVNGDAARRLNASVGDQVTVVLGEG"
     gene            3978..4586
                     /locus_tag="AC16CB_14515"
     CDS             3978..4586
                     /locus_tag="AC16CB_14515"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3343732.1"
                     /translation="MNSKKDVAFSLEALKAGDRLEFARLVETYYEMIYRLAIKMLNNP
                     QDAEDVLQETFLKALRGIRKFDGRSSISTWLYRIATNEALMALRRQKPNLISTDEPLE
                     TEEGEQEPIQIVDWCCLPEPELMSGEAQGYLEQAISHLSPNLRAVFVLRDIEGLSTQE
                     TGEVLNLSETAVKTRLSRARLRLREDLSGYFGEKLFEGKRVE"
     gene            4583..4846
                     /locus_tag="AC16CB_14520"
     CDS             4583..4846
                     /locus_tag="AC16CB_14520"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR3343733.1"
                     /translation="MKPEKCRHLLGSLSDYIDGTLEEQLCTQLERHLADCPDCRIVVD
                     TLNMTVSLYKQSADLTSVPEEVRQRLFRCLNLEDLLGNPKTPF"
     gene            4854..5060
                     /locus_tag="AC16CB_14525"
     CDS             4854..5060
                     /locus_tag="AC16CB_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343734.1"
                     /translation="MNHSIAQPPSEIDVSMPPLPGENTLIMLSKAPRPGDLCPHCGQE
                     QLDYDGTLNLACPRCGLILGGCFT"
     gene            5197..5865
                     /gene="pdo"
                     /locus_tag="AC16CB_14530"
     CDS             5197..5865
                     /gene="pdo"
                     /locus_tag="AC16CB_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_394797931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5641..5643,aa:Sec)
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HIR3343735.1"
                     /translation="MTTKLLNETVAEQVRELFDQLQEPVEVLFFGQEAGCEYCEDTLQ
                     LIQEVVALSDKLGLQVFDLDKDAAVAEQYKVDKAPGMVIAGRDGDQILDYGIRLAGIP
                     AGHEFSSLVHDMILVSGRDSGLDSRTRAFLAGLEQPVLLQVFVTPTUPYCPQAVVLAH
                     RMALESPMVEAEMIEAMEFPELSMRHGVSGVPQTTINDGAGNVVGAVPEPHLVIEIQQ
                     ALAG"
     gene            5862..6416
                     /locus_tag="AC16CB_14535"
     CDS             5862..6416
                     /locus_tag="AC16CB_14535"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR3343736.1"
                     /translation="MRPGRPQLLALALIGAGLVILGVAVWVLLPSLTAASQEEISAIP
                     VQVDYAAPDLSLTDLAGNPVSLNDYRGSVVLVNNWATWCPPCKAEMPALQAYYEAHQG
                     QNFTIIAIEAGQPASEVADFVRRYGLTFPVWTDPTLKATVAFRNPGLPSSYVIDASGT
                     VRLAWAGAINLDKLEEHVTPLLEE"
     gene            6419..6889
                     /locus_tag="AC16CB_14540"
     CDS             6419..6889
                     /locus_tag="AC16CB_14540"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR3343737.1"
                     /translation="MEAKVIWQGGMKLDGLADSGLHLPLGSRPAGDGDDQGFLPMELL
                     ATGLAGCTAMDVISILQKKGQDVTGFEVQVHIQRAPEHPRVFTHALIEYFVTGRRVDE
                     AAVVRSIELSATRFCPALAMFKKVFSIEMKYHVFEAGDDDKRQLVKSGEYAYPV"
     gene            complement(6891..8204)
                     /locus_tag="AC16CB_14545"
     CDS             complement(6891..8204)
                     /locus_tag="AC16CB_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3343738.1"
                     /translation="MPVERPTGMFGFTIVWIGQFVSLLGTSMTAFSLTIWAFELTGSA
                     TALALVGFFYVTPMLIFSPFAGAIVDRANRKWMMALSDLASGMATLGILGLYLSGQLQ
                     IWHLYVANVIIGLFQSFQWPAYSAAISVMLPKEHYGRANGMMSLAESGSGIFAPLLAG
                     AVLGIIGLGGVLFIDVATFLFAIATLTVIHVPQPTVTEDGLQGRGSLLQEAVFGFRYI
                     LARPSLLGLQSVFLLGNFFSSAMFIMLAPMILARTSNNELIYGSVMSAGAVGGVIGGL
                     AMSAWGGPKRRIHGVLGGWIVASILDGVVMGLGRDLPVWAAASLLGAFFIPVINGSNQ
                     AIWQAKVAPDVQGRVFSIRMLIAWFVNPVAMLVVGPLADYVMEPAMQAGGTLSGTFGW
                     LVGTGPGAGIALIFIFSGLMASLISMGAYTVTAIRDVESILPDHS"
     gene            complement(8211..9701)
                     /locus_tag="AC16CB_14550"
     CDS             complement(8211..9701)
                     /locus_tag="AC16CB_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR3343739.1"
                     /translation="MKSQFNFRLLSLALIPALLLSSCAPQIVSTSSGAAAEAAPPLQP
                     TPEASAPPPMPQRPVYAPGELVDYTAQTGDTLPSLAVRFNTSVDEIRQANPIIPESAT
                     SMPPGMPMKIPIYYLPFWGSSYQILPDSLFVNGPAQVGFDTAKFIAEQPGWLKGYTEY
                     AADENRSAANMIDLVALRYSVSPRLLLALLEYQSGALSQAEPSEEAQTYPMGYDRRER
                     RGLYRQLIWAANLLNNGYYGYRTNHLTSLDYEDGRMERFDPWQNAATVGLMNFFNTLL
                     PKDAYLQAISSEGFAATYASLFGDPWAADHPHIPGSLEQPEFALPFKAGEIWAFTGGP
                     HTAWGIGDPLSALDFAPPSVAGGCQETDLWATAVADGVIARSETGEIALDLDRDGDER
                     TGWVVFYLHLATQGRAPLGAEVVAGQPVGHPSCEGGSSTGTHVHIARKYNGEWMPAEG
                     TLAFNLEGWQARNGSQPYQGTLTRLSRTVTACVCSNRDTFIQSEKR"
BASE COUNT         2307 a         2748 c         2818 g         1908 t
ORIGIN      
        1 cctttggcgc tctccagctc gtaaccgcgc gcccgctggg catccacggt gatcatgaaa
       61 tcgcggccca gggtgggaat gaagcgaaag gccaagtcca tcgtaaatga agctttatcc
      121 ggaagaccca tgcggcgaaa agtaaccccg tagatgctgg gatccatagt gtagggaatc
      181 ggaatagcca ggatggccat ggtaagcatg cgtacgatct gggtaatggc aaagaacgag
      241 cgcaccacag tgatggttag cgtgatgctc cacggagtga acggtatgtg tagcgtggga
      301 gacttgtaaa gagcagcaga ctggtcttgc aacacagcag tcggcccgcc gcgcccagct
      361 aacaagccgt tgattcccac aatgaagatc accaagaaga ggatgaacgc ccaggcccgc
      421 cagacatcgc gccactcaat gcgggccatc aggtagagga ccatggtcag gataaacagc
      481 ggcagcaaga gacggatatc ccagacctgg gcgcccgaaa cagcaaggat gatgcacgcc
      541 aggaaagtta acctggcgcg cgggtcaagc cgttgaataa aagtatcgcg cgggcgatac
      601 ttccaggcga cgagcaccta cctgcctctg cgagaagtta cccctgcgaa ggcgaccatc
      661 aagataggta gcagcactgc ggtcaccacc agattgccaa taaaggccgg cgtgaagtag
      721 ccgaccagcg cagtgcttag gtcaatacct ccaacgaaca tctcagtgag cgaggcaaaa
      781 agcatgccca cagcgatgcc cagggcaccc cagccgatgc caataccgat cgaccgccca
      841 ctgcggtaat cctggatcat cgccatagcc aaccctggga tcatgcccat caagccgttg
      901 cctacgctcc agttccagaa gaaaccccaa ccagaaagcc catcacccag ggtgttgcca
      961 accaagccag cgatcagccc tacccaaggg ccataggcga taccgaagaa aatcaacacc
     1021 gccactgcag gcctaaaggt tacattgcca gcagctggta gggggatcac gttggtgatc
     1081 cacgagaaaa cgccatacag cgctgcaccg attgccccgt agacgacctg ccgggtgcta
     1141 aaactccaca gaccagattg attatccatt gctttgctcc ttttcgatcc tttcagaata
     1201 agaggtttag attgaggcag cggctgcctc acataattaa acccgctttt gagcaaatgg
     1261 ttgcacccat ttgattgttt taggcaaaaa actccttcac ttaagcatac acagccagag
     1321 cctctgcagg gaggaagcga ccggtcttgg acagtaaatc caaattcatg cgcagtaacg
     1381 atgtggggcg gacgcgatag cgctgcaaaa gctcaaaatc gcgcaaaact tgctcggggg
     1441 gtccgtcggc taccacgcgg ccttcaccca ccaggatgac ccggttggca taggtgacag
     1501 ccaggtccag gtcatgagta ataaaaaggg tggctgcgaa attagcggtc aacaacgatt
     1561 tgccgtcttc gccgctctcg ccgcaaagaa tttccatgaa ttgggtatag ttggcgtaat
     1621 cctgcccggc ggtcggctcg tccagcacca tgatcattga acgcatggcc atcacggagg
     1681 caatgccggt gcgtttctgc tgcccaaagg atagtcctaa aggagggtat tcttccaatc
     1741 cagtgagatt tactgctgct acgctggcga ctacgttctg ctcgatctca tcacgagaat
     1801 agcccaggtt agcaggaccg aaagctaatt cttcacggac tgttggggcg aataacatgt
     1861 gcgtggggct ttggaagacg taacccactt tgcgcgccgt ctgggcgaca gtcattttac
     1921 gcgtatctac cccttccact tgcactaaac cacgagtagg cttcaacagg ccaatggcat
     1981 gcttgaccag ggtagattta cccgccccgt tcggtccaag gacggcgatc aaatcaccct
     2041 ggtggatctt tagattaacc tcatgcaata cctcggggcc atccccatag gcgaatgaga
     2101 catctttgaa ctcaatgaga ggcgcacctt ccgccactgc ctggggaggg ggctgcagcg
     2161 ccgactgcgt gggttgtgtt tgatgcactc gttgcaccac ccatggagca ggtagcttga
     2221 cctcacggtg gtcgatctct ggggggagcg aatccacagc gcccaagtag cgcacccgtc
     2281 cggctgacat atacagcaat cgatcggggc gcgctgccag agcgtcttca acgcggtgct
     2341 cgacaagcaa gatggtcgag ccctcatctg ccaggctgcg gaaaatcgcc agggcttcat
     2401 gggctgaggc tggatccaga gaggccaggg gctcgtccag aagcagaatc gaggggcgca
     2461 tcaccaacag gccagccaga gcgactttct gtttttctcc cccagataac gtgaaggtat
     2521 cccggccgcg caagtgctcg atattcaagc gggcgatcac ctggtcgata cgctcaccga
     2581 tctcttgggt tgagagtccc agattctctg gtccaaaagc aatctcattc caaacatttg
     2641 aggcaacgat ctgccgttca ggatcttgaa gcaaggttcc aataacctgg gcaacctcca
     2701 ctagcggcat cgcagagact tccttgccgt ggatgaacac cgatccagcg cgatcacctc
     2761 tataactgcg cgggatcagc ccgttgatgc agcgcgccag ggtagtcttg ccgcaaccgc
     2821 tggcgcctgc caccaagagc agctcgccgc gccggagctg gaatgacacg tcctggatag
     2881 caagctcagg ccggctgtga tagcggaaag acagattttc aacaacaagc gggtattctt
     2941 cgctcaactt cacccttcac caagaacaac agtcacttga tctcctaccg aggcgtttaa
     3001 tcgccgggca gcatccccgt tgaccactga gatgatcagg ttgccagtgc tgccatataa
     3061 agctaccagc tcaccgggag tgcgctcgcc gaaagtgcgc accaaacccg tgattatcac
     3121 atcgcgcagg ccaactttca catgatcagg atagcctagg ttttctaccc ggatgctggt
     3181 ggagatatta ccgaaatgat cgatgtggat gacctcgccc tgccaaccag atggggtggt
     3241 ttgtggcttg gggagagaca gccgtactgg atcatggata ggcgtaccca gggcatacaa
     3301 gggcacgcct ctcgccaaat gagcggcgat aggggcgaaa acatccctgc catgaaagac
     3361 atggctgact ttcggcaacc aatactggcg cttatccgtg tggacgatct caaccgtcca
     3421 atcctcggcc tctgcatact cgagcagccc gctgatcacc ccattatccg gtcccacaaa
     3481 gtattgcatg cccaggcggg cggcaatgtg ccggcggttg gtgcccacac ccggatccac
     3541 caccaccaag tggatactgc ccgcaggaaa gtaaggggca gaacgcgcta gaattaggga
     3601 ggcttccaat acattctgcg ggctgattgt atgactcaaa tcggcaatct gagcatcggg
     3661 ggaaatattc cagatgaccc ccttcatcac acccacattg ccatctttaa taccaaagtc
     3721 ggtcattaag gagataatgc tcatgtgcct tgctttatag cacgcaagtc tgagaccgtc
     3781 aatatgataa aataatttgg tcgtatctct tacctagacc tcttgctgcc ctgcttgcat
     3841 tagaccagcc gccatcctat tgcgagcagg gtctgtggtc tagggaggga ggggagacag
     3901 cgcacaagcc ggctaagccc cttgacagcg ccctcacacc tgggtgtgaa gtaacttttt
     3961 cgataggatt gcatctaatg aatagtaaaa aagatgttgc gttctcgctg gaggcgctga
     4021 aggccggaga ccggttggaa tttgcccggc tggtagagac atattacgag atgatttacc
     4081 gtctagcgat taagatgctc aacaaccccc aggatgctga agatgtgctc caggagacat
     4141 ttctcaaagc tctgcgaggc atccggaaat ttgacgggcg ctcgagtata tccacctggc
     4201 tctaccgaat agccaccaac gaagcactga tggcactgcg ccggcagaag ccgaacctca
     4261 tctcaaccga cgagccattg gagacggagg aaggcgaaca ggaacccatt cagattgtgg
     4321 actggtgttg tctgcccgag cccgaattga tgtcaggaga ggcgcaaggc tacctggaac
     4381 aggcaatcag tcacttgtcc cccaatttgc gagccgtatt cgtgctgcgc gacatcgaag
     4441 gcctgtccac gcaggagact ggagaagtgc tgaacctgag cgagacagcc gtgaaaaccc
     4501 gcctgtcacg cgcccgcctg cgcttgcgcg aggacttatc aggatatttt ggtgaaaagc
     4561 tgtttgaagg gaagcgtgtg gaatgaaacc tgaaaaatgc cgtcatctct tggggtcgct
     4621 ctcggattac atagacggca ccctggaaga gcaactgtgc acgcagcttg aacgccacct
     4681 ggcggattgc ccagactgcc gcattgtggt ggatacgctg aatatgaccg tctccctgta
     4741 caagcaatct gctgacctga cttcagtgcc agaagaggtg cgccagcgcc ttttccgctg
     4801 cctgaacctg gaagatttgt tgggaaatcc aaagacacca ttttagagac gtaatgaacc
     4861 actcgattgc ccagccaccc agcgaaattg acgtttcaat gccgccatta ccgggggaaa
     4921 acacattgat tatgctttct aaagccccgc gccctggtga cttatgcccg cactgcggac
     4981 aggagcagct ggattatgac ggcacactca atctggcatg cccacgctgc ggattgatcc
     5041 tgggcgggtg ttttacttga tgatttgttt ctcgccccgt tggggtgcaa acaagaagta
     5101 gagagtttac acagttgaac aaaggtgtgg gcacatagtg ctccttacta ataataatca
     5161 accgtgtaat ctctgaaaca ggagtgaatt atcgaaatga caaccaagct attaaacgag
     5221 accgtcgcag agcaggtgcg tgagctattc gaccagttac aagaaccggt agaggtgctt
     5281 ttcttcgggc aggaagcggg ctgcgagtac tgtgaggata ccctgcaact gatacaggag
     5341 gtggtagccc tatcggataa gctgggcctg caagttttcg atctggataa ggacgcggcg
     5401 gtcgcagagc aatacaaggt cgataaagcc cccgggatgg tcatcgccgg tcgcgacggc
     5461 gaccagatcc tggattatgg catccgttta gccggtattc ccgccggtca tgagtttagc
     5521 tccctggtac acgatatgat cctggtttct ggacgcgatt caggcctaga ctccaggacg
     5581 cgcgccttcc tggccgggtt ggagcagccg gtgctgctgc aagtcttcgt cacccctacc
     5641 tgaccgtact gcccgcaagc cgtggtgctt gctcaccgca tggcgctgga gagcccaatg
     5701 gtcgaggcag agatgatcga ggcgatggaa ttccctgaat tatccatgcg gcacggtgtg
     5761 agcggggtgc cgcagaccac catcaacgat ggagcgggca acgtggttgg cgccgtaccc
     5821 gaaccgcacc tggtgatcga aatccaacag gccttagctg gatgagacct ggacgcccgc
     5881 aacttttggc gctggcgctg atcggcgcgg ggctggtgat attaggggtg gcagtttggg
     5941 tgctgctgcc cagcctgacg gctgctagtc aggaggagat ctccgccatc cctgtgcagg
     6001 tggattatgc cgccccggac ctgagcctta cagacctggc tggcaaccct gtctccttaa
     6061 atgattatcg agggtcagtg gtgctggtca ataactgggc cacctggtgc ccaccgtgca
     6121 aggcagaaat gccagcccta caagcctatt acgaagccca ccaggggcag aacttcacca
     6181 ttattgccat cgaggcaggg cagccggcct ccgaggtagc ggactttgtg cgccgctacg
     6241 ggctaacctt cccggtttgg accgacccaa ccctgaaagc cacggtcgca ttccgcaatc
     6301 ccgggctgcc tagctcatat gtgatcgacg ccagcggtac ggtgcggctt gcctgggcgg
     6361 gggccatcaa cctggacaag ctcgaagagc atgtgacacc tttgttggag gagtagactt
     6421 ggaagcaaaa gtgatctggc agggaggtat gaaactggat ggcttggcag acagcggcct
     6481 acacctgccg ttggggtcca ggcccgccgg ggacggtgat gatcaggggt tcctgccaat
     6541 ggagctgctc gccaccggct tagcgggctg cacagcgatg gatgtgatct ccatcttgca
     6601 gaaaaagggc caggatgtga ccggttttga ggtgcaagtc cacatccagc gcgcgccgga
     6661 gcacccccgg gtatttaccc acgcgctgat tgagtatttc gtcaccgggc gacgggtgga
     6721 tgaggcggca gttgtgcgct caatcgagct ttcagccacg cgcttctgcc cggcactggc
     6781 catgttcaag aaagtgttct caatcgagat gaaataccat gtcttcgagg cgggggatga
     6841 tgacaagcgc cagctggtca agagcgggga atatgcatac ccagtttaac ttagctatga
     6901 tctggcaaga tgctctctac atctcggata gcggtaacgg tgtaggcacc catgcttatc
     6961 aggctggcca tcaggcctga gaagataaag atcagggcga tgcccgcccc aggccccgtg
     7021 cctaccagcc agccgaatgt gccgctgagc gtaccgcccg cctgcatagc gggttccatg
     7081 acataatccg ccagcggtcc gaccaccagc atcgccacag ggttgacgaa ccaggcaatc
     7141 agcatgcgga tggagaacac ccgtccctgc acatcgggag ccaccttggc ttgccagata
     7201 gcctggttgg agccgttgat gacggggata aagaaagccc ccagcaggct tgcggcagcc
     7261 catacgggca ggtcgcgccc taaacccatc accacaccat ctaatatact ggccacaatc
     7321 caaccaccca gcacgccgtg aatgcggcgc ttgggcccgc cccaggcgct catcgccaac
     7381 cctccaatca ccccgcccac cgctcccgct gacataacac tgccatagat tagctcgtta
     7441 ttgctggtgc gcgccagaat catgggagcc agcatgatga acatagcact ggagaagaag
     7501 ttccccaata gaaacaccga ttgcagcccc aataagctgg gacgggccag gatataacgg
     7561 aacccgaaaa cagcttcctg tagcagactg ccgcgcccct gcaaaccgtc ttctgtcact
     7621 gtgggttggg gcacgtggat caccgttaag gtagctatgg cgaataggaa ggtggccaca
     7681 tcgatgaata gcacgccgcc cagcccgata attcccagca ctgccccagc cagcaggggg
     7741 gcgaatatac ccgaaccgga ctcggccagc gacatcattc cattggcgcg cccatagtgc
     7801 tccttgggca acatgacact gatggcagct gaataagccg gccattggaa ggactggaag
     7861 aggcctatga tgacgttggc tacatacaag tgccagatct gtagctgtcc agacaaatac
     7921 aggccaagga tacccagtgt agccatcccc gaagccaggt cgctgagcgc catcatccac
     7981 ttgcgattag cgcgatcgac gatcgcgccg gcaaacgggc taaagatcaa catgggggtg
     8041 acgtagaaga aaccaaccag cgccagagcc gtggcggagc cggtgagctc aaaggcccaa
     8101 atagttagag aaaaggcagt catgctggtg cccagcaacg acacaaactg cccgatccag
     8161 acgatcgtga agccaaacat tccggtgggt ctttctactg gcatggtgtt ttagcgcttt
     8221 tcggactgga tgaaggtatc tcggttagag catacacagg cagtgactgt gcgcgacagg
     8281 cgggtgagcg tgccttgata aggctggctg ccgttgcgcg cctgccatcc ctccagattg
     8341 aaagccagcg ttccttcggc aggcatccac tcgccgttgt acttgcgggc aatatgcacg
     8401 tgagtgcccg tgctgctgcc cccttcacag gaaggatgtc ccacaggctg cccggctact
     8461 acctcggccc ccaggggagc gcgcccttgt gtcgccaggt gcaggtagaa gacaacccag
     8521 ccggtgcgct cgtcgccatc tcggtccagg tccagggcga tctcgcccgt ttcagagcga
     8581 gcgatcacgc catccgccac agcagtcgcc cacaggtctg tctcctggca gccgcccgcc
     8641 acagacggtg gggcgaagtc cagagcagac agggggtcgc cgatgcccca ggcagtgtgc
     8701 ggtccgccag tgaaggccca gatttcacca gctttgaagg gtaaagcgaa ctcaggttgt
     8761 tccaggctgc cagggatgtg cgggtggtcg gctgcccagg gatcgccgaa gagagacgca
     8821 tacgtggcgg cgaaaccctc ggacgagata gcctggaggt aggcgtcttt aggcaacaga
     8881 gtgttaaaga agttcatcag gccaacggtc gccgcgttct gccaggggtc gaagcgctcc
     8941 atgcgcccgt cttcgtaatc gaggctggtc agatggttgg tgcgataccc gtagtagccg
     9001 ttgttgagca ggttggcagc ccagatgagc tggcggtaca ggcctctgcg ctcgcgacga
     9061 tcgtaaccca tcgggtaggt ctgcgcctcc tcggagggtt cggcctggct gagcgcgccg
     9121 gactggtact cgagcaatgc caataacagg cgcgggctga cgctatatct gagggccacc
     9181 agatcgatca tattggcagc ggagcggttc tcatcggcgg catattcagt gtagcccttg
     9241 agccagcccg gctgttcagc gatgaacttg gcggtgtcga aacccacctg cgccgggcca
     9301 ttgacgaaca ggctgtcggg caagatttga taggagctgc cccagaaggg caggtagtag
     9361 attgggatct tcatgggcat gccgggcggc atgctggtcg ccgattcagg gatgatcggg
     9421 ttggcctggc gaatttcatc cacgctagta ttgaagcgca cagccaggga aggcaaggta
     9481 tcgccggttt gcgcagtgta atcgaccagc tcgccgggcg catagaccgg gcgttgtggc
     9541 atagggggtg gtgcagacgc ttcaggggtt ggctgcaacg gtggagctgc ctctgccgcc
     9601 gcgccagagg aggtggagac gatttgcggg gcgcaagagg agagcagcaa ggcaggtatg
     9661 agagcgagtg atagaagacg aaaattaaac tggcttttca cgggagggaa ttataaccca
     9721 cacctattcg gcaatagagt atagcgcaga gatgctcaag cccaatcaaa catcgacatc
     9781 a
//
LOCUS       DBNGSX010000491         2879 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_534351, whole genome shotgun sequence.
ACCESSION   DBNGSX010000491 DBNGSX010000000
VERSION     DBNGSX010000491.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_534351"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="AC16CB_14555"
     CDS             <1..192
                     /locus_tag="AC16CB_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343740.1"
                     /translation="RGTYDQYGKGIAWEKMRARDPITWELGQIVRQPRSSLDQLTHTL
                     RLRRSRISDRWAGTRSVQT"
     gene            511..1677
                     /locus_tag="AC16CB_14560"
     CDS             511..1677
                     /locus_tag="AC16CB_14560"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019350.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3343741.1"
                     /translation="MKRRLSFSNAVNHLSSSRYGVDQLIHELLEMLRTQEDADACFLL
                     MTDTQSGEHLIYDAKGGVAPRRSGGSRVGAELFSSTFALPPDAAVLYRRRRWPRVGGQ
                     GFAYDLMTLESQPVTQGLLSELANFLETTSFISLPMRVTKPSLGRIYLTSTRRRFSRT
                     DIGCLAQAVDQTALVIKNTQLLDRLVMAAATNERRKISRDLHDGTIQPYIGLKLGLEA
                     LRRKRGMSDAIASEVDQLTKMAEDGISQLRRYVGRLRSGKCQSGHDLSLPAVRRQVER
                     FTEFYGLNVKVGGDNDVVVPAHLSEEVMHIVREGLSNIRRHTVAEDAAINLREGKGRL
                     VVEIIQSKERLNGHNGVVPFFPRSLNERARELGGRVNVERRPRGETAVLVEVPL"
     gene            1697..2386
                     /locus_tag="AC16CB_14565"
     CDS             1697..2386
                     /locus_tag="AC16CB_14565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3343742.1"
                     /translation="MRACAVEPIRVLIVDDHKSVLWGLEKLIDSEQPLMRVVGKATRC
                     AEALVAIEHHQPDVLLLDVDLEEDNGLDILDALRGRSYPNVLIVSGLRDAQMLESAMR
                     KGARGVVCKAESAESILKAIVHVHKGEFWLPRASMAKILTSALDHGNAGHETAPATAE
                     ATLTPTERRIIAAVVQYKGAQNKVIAGALGISCNTLRNHLASIYNKIDVHSRLDLYLY
                     AKERKLEKHVA"
     gene            2662..>2879
                     /locus_tag="AC16CB_14570"
     CDS             2662..>2879
                     /locus_tag="AC16CB_14570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3343743.1"
                     /translation="MQNKRSETIRILLVDDHDHILWGLRKLIESEEPGMAVVGTARTV
                     SEALEAIRERKPDVVLLDIYLGEESSVD"
BASE COUNT          649 a          735 c          870 g          625 t
ORIGIN      
        1 cgcggtactt acgatcaata tgggaagggc atcgcttggg agaagatgag agcgcgtgac
       61 cccattacgt gggaactagg ccagattgtc cggcaacccc gttctagtct cgaccagttg
      121 acccacacat tacgactacg tcggagcagg attagcgatc gatgggcagg gacacggtcc
      181 gtgcagacct gaggttcgtt ggactgaaaa atagtgcgaa ctacacgcct tgcttcgtcg
      241 ggcttaggac aactttaggc atgaggtggg agggtcggaa tgtcgggaag actagggttt
      301 tggggtgaaa caaggttccg gatgacggtt tgtggtcact agtgacgact tctcatcagg
      361 acgactgcag gaacagagac cgcaatgggc ccgatcatac aggcttggta ttcaccggga
      421 ttcgatggga tctggggttt gctcggactt ggtgccttgc tattgctctt cgcatttatc
      481 actgctagat ggagcaaatc cagggctagt ttgaaacgcc gccttagttt ttcgaacgct
      541 gtcaatcatc tttcaagttc gcgttatggt gtggatcagc tcatacacga gcttttagaa
      601 atgttgcgca cacaagagga cgccgacgct tgttttctgc ttatgacgga tactcaatca
      661 ggcgagcatc tgatttatga tgcgaagggt ggcgttgcgc caaggcgctc gggtggatcg
      721 cgcgtgggcg cggaattgtt ctcatccacg ttcgcattgc cgcccgatgc ggcggtgctt
      781 taccgtcgac ggcgttggcc ccgggttggt gggcaagggt ttgcctacga cttgatgact
      841 ttagagtccc agccggtcac gcagggcctg ttgtcggagc tggccaattt tctggagacc
      901 acctcgttca tcagcctgcc catgcgtgtt accaagccct cgcttggccg catatacctg
      961 acgtcgaccc gtcggcgttt cagccgaacg gacataggat gcttggcgca ggcagtcgat
     1021 cagactgcgc tggtgatcaa gaacacccag cttctagacc ggctggttat ggcagcggcg
     1081 acaaacgaga gaaggaagat ttcgcgggac cttcacgatg gaacaattca accctacatc
     1141 ggtctgaagc tcggcctcga agctctgcgg cgcaagcgcg gaatgagcga cgcaattgct
     1201 tccgaggtgg atcaactcac aaagatggca gaagacggga tcagccagct gcgtcgttat
     1261 gtaggccgct tgcggagcgg aaaatgccag agtggacatg atctgagttt gcctgcggtg
     1321 cggcgccagg tggaacggtt caccgagttt tacgggctca acgtcaaggt gggcggcgat
     1381 aacgacgtcg tggtccctgc gcacttgtcg gaggaagtca tgcacatcgt tcgtgagggg
     1441 ctttcgaaca tccggcgcca cacggttgcg gaggatgccg ccatcaattt gcgcgagggc
     1501 aaggggcggc tggtcgtgga aatcattcag agcaaggaaa gattgaacgg ccacaatggt
     1561 gttgtgccct tctttccccg ctcgcttaac gaacgagcgc gagaactggg cggccgagtc
     1621 aatgtggaac ggcggccgcg cggcgagaca gcggtcctgg tcgaagttcc tctttgaaat
     1681 gaaaaagagt agataaatgc gcgcctgcgc ggttgagccc attcgtgtgc tgatcgtgga
     1741 tgaccacaaa agcgtgcttt gggggcttga gaagctaatc gatagtgaac agcccttgat
     1801 gcgggtcgtc gggaaggcaa cccgttgcgc ggaagccctt gtcgcgatcg aacaccacca
     1861 gcccgacgtc ctcctcctcg atgttgatct tgaggaagat aacgggctag acattctgga
     1921 tgcgctgagg gggcggagct atcccaacgt gctgatcgtg agcggtctcc gagacgccca
     1981 aatgcttgag agcgccatgc gaaagggagc ccgtggagtg gtgtgcaagg cggaatcggc
     2041 agagagtatc ctcaaggcga tcgtccacgt gcataaaggg gagttttggc tgcctcgtgc
     2101 cagcatggcg aaaatactca cctccgcgct tgaccatggc aacgctggac acgaaaccgc
     2161 gccagctacg gcagaggcaa cactcactcc tacggagcgt cgtattatcg ccgcggtagt
     2221 tcagtacaag ggcgcgcaaa ataaagtcat cgccggcgcc ctcggtatca gctgtaatac
     2281 gttgcgcaat catctcgcct cgatctacaa caagattgat gtgcatagcc gcctcgacct
     2341 ttatttgtac gctaaagagc gcaaactcga aaaacacgtg gcctgagccc tttagcacag
     2401 cagtaattac tttagacgga tcttggcaag gacgaccctt ctagagggcg tccgtttctt
     2461 cctcccccgc tgtagccctt ggccatcgct tagtgcagct gcactaagcg ctcttagtcc
     2521 acccagcaga tcgggccggt cagtttttac ttagaaaaaa agttgctttc gctcatgtgg
     2581 aaaagcatcc gcttgcgtat tctcctctac aacgcaggat gtagttacga caaatcgcgg
     2641 ctcgaaacga tgagtattta aatgcaaaac aaacgaagcg agacgataag aatcctgctg
     2701 gtggacgatc atgaccatat tctttggggc ttgcgcaaat tgatcgagag cgaggagcca
     2761 ggcatggcgg ttgtagggac ggcgcggacg gtttctgagg ccttagaggc gatccgcgag
     2821 cgcaaacctg acgtagtcct gctcgacatc tacttgggtg aagagagcag cgttgacca
//
LOCUS       DBNGSX010000492         2306 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_395362, whole genome shotgun sequence.
ACCESSION   DBNGSX010000492 DBNGSX010000000
VERSION     DBNGSX010000492.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_395362"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="AC16CB_14575"
     CDS             <1..275
                     /locus_tag="AC16CB_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR3343744.1"
                     /translation="LYTAVINGVQEIYTMDLDTGDEWELSGDTSGEDHPRWSPDGHYI
                     VYDAKHGPGSNGGVDSEIYLFDYWSDQAIPIRLTDNDVDDDAPAVK"
     gene            285..677
                     /locus_tag="AC16CB_14580"
     CDS             285..677
                     /locus_tag="AC16CB_14580"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl trahydropterin synthase family
                     protein"
                     /protein_id="HIR3343745.1"
                     /translation="MYTVAVKRDFIAQHYLIGGDWGAENQLHAHHYQAEVQLEGSELD
                     QHGYLVDIVDIENALDTLVARYRDRALNDLPEFAGLNPSIEHFSRILCQALVAHIQSA
                     NLRAVTVKLWENEIAWASYRAEDLNSRG"
     gene            726..1826
                     /locus_tag="AC16CB_14585"
     CDS             726..1826
                     /locus_tag="AC16CB_14585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3343746.1"
                     /translation="MKLGLVIYGSLDSLSGGYLYDRKLVNNLTRLGDTVDVIALPPGQ
                     PTRAGWLNYVRHLGDNLSQGLSRRLERLEVDILLQDELNHPSLAWVNRRLRGRVAYPV
                     VSIVHHLRSDELRPAWQNRFYAWVERIYLKSVDGFIYNSRTTQGRVERLAGKGLPSVV
                     AYPAANHLNPQIEQVEIIQRARQAGPLSLFFVGNLIPRKGLHTVLEALSRLQKGEFVL
                     DVAGSLTSDPVYVDAIRQQIEKLNLSLAVHLHGALGDSQLVDNYRRAQVLVLPSSYEG
                     FGIAYLEGMGFGLPCIGTTAGAAGEVIEEGQTGLLIQPGDSVSLANHLRRLADDRTLL
                     ETMSLAARRRYLSHPTWEQTAEKTRSFLETLI"
     gene            1841..>2306
                     /locus_tag="AC16CB_14590"
     CDS             1841..>2306
                     /locus_tag="AC16CB_14590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3343747.1"
                     /translation="MEYSFPRYLLSKQSVDDRALNPRVYAALHSSLPYQSPLRITEVG
                     AGIGTMLSRLIHWGLLDKADYTVVDETPENIDYAREWLPRQAREAGFEVEESGNSLRL
                     YDASRDIRVHLLASDVFEFAQTQPPRAHLLIANAFLDLLPLPESLTSLFSLVE"
BASE COUNT          479 a          658 c          645 g          524 t
ORIGIN      
        1 tgctctatac ggcggtcatt aatggtgtcc aggagattta tacaatggat ctcgacacgg
       61 gagatgagtg ggaattgagc ggtgatacca gcggtgaaga tcacccgcgc tggtcaccgg
      121 atgggcatta cattgtctac gatgctaaac acggcccggg atcaaacgga ggggtggatt
      181 ctgagattta ccttttcgat tattggtctg accaagccat cccgataagg ctaaccgata
      241 atgatgtgga tgacgacgcg cctgcggtaa aataagcttc agttatgtac actgttgctg
      301 tcaagcgcga ctttatcgcc cagcattacc tgatcggcgg ggattggggg gcggaaaacc
      361 agcttcacgc tcatcactac caggcagaag tgcaattgga aggctctgag ctggatcagc
      421 atggctacct ggtggacatc gtggatatcg agaatgcttt ggataccctg gtcgcccgct
      481 accgcgaccg ggcactcaac gatttgccgg aattcgctgg tcttaacccc agcatcgagc
      541 atttctctcg catcttgtgc caggcgttgg tcgcccacat ccaatccgcc aatttgcgcg
      601 ccgtcacggt taagctatgg gagaacgaga tagcctgggc ttcctatcgc gcggaagatc
      661 tcaatagtag gggctgaagt gcccttcgat attcaacctg ccagtacttc accgtttaac
      721 cacctatgaa gctgggcctg gtcatctacg gcagcctgga ttccctgtct ggcggttacc
      781 tgtatgatcg caagctggtc aataacctga cccgcctggg cgatacggta gacgttatcg
      841 ccttgccccc tgggcagccc acaagggcgg gctggcttaa ctacgtccgc cacctggggg
      901 ataacctgtc tcagggatta tcccgccgcc ttgagcgcct ggaggtcgat atcctgctgc
      961 aggatgagct caaccaccca tccctggcgt gggttaacag gcggctgcgc ggtcgcgtgg
     1021 cataccccgt ggtttccatc gtgcaccacc tgcgcagcga tgagctccgt cctgcctggc
     1081 agaaccgttt ctacgcctgg gttgagcgca tctatttgaa gagcgtggat ggcttcattt
     1141 acaacagccg cacgacccag ggcagggtgg aaagactggc tggtaaaggg ctgccgtcgg
     1201 tggtcgctta cccggcggct aatcatctga atcctcagat cgagcaggtt gagatcatcc
     1261 agcgtgctcg ccaggcaggc cctctatccc tgttctttgt aggcaacttg atcccgcgca
     1321 aaggcttgca taccgtgctc gaggccctct cccgcctaca gaagggggaa tttgtcctcg
     1381 atgttgccgg cagcctgacc agcgatcccg tctatgtgga tgccatccga cagcagattg
     1441 agaagctaaa cctgagttta gccgtccacc tgcacggcgc gctgggagac tcgcaattgg
     1501 tggacaatta ccgccgcgcc caggtattgg tgctaccctc ctcctacgag ggctttggta
     1561 tcgcctattt ggagggtatg ggctttggtc tgccctgtat cggcacaacg gccggcgcgg
     1621 ctggggaagt catcgaagaa ggtcagaccg gtctcttgat acagcctgga gactctgtat
     1681 cgctggccaa tcacctgcgg agactggcag atgatcgcac actactcgag accatgtccc
     1741 tggctgctcg ccggcgctat ctatcccatc ctacctggga gcaaacagca gaaaaaacca
     1801 ggagtttttt agaaacgctt atctaatctg gaatattcaa atggaatact cttttccaag
     1861 gtacctgctg tccaaacaga gcgtggatga ccgcgccctc aaccctcgcg tctacgccgc
     1921 tttgcattct agtctgccgt atcaaagccc gctgcgtatt accgaagtgg gagcggggat
     1981 cggcactatg ctaagccgcc tgatccattg gggtctgctc gataaggctg actataccgt
     2041 tgtcgatgag acccctgaga atatcgacta cgccagagaa tggctgccgc gacaggcgcg
     2101 ggaggcggga ttcgaagttg aagaatcggg taattcgttg cgcctgtacg atgccagccg
     2161 ggatatccgc gtgcacttac ttgcctccga tgtcttcgaa ttcgcccaaa cccagccccc
     2221 tcgggcgcat ttgctcatcg ccaacgcctt tcttgacctg ctgcccctgc ccgagagcct
     2281 gacgagcctg ttctcattgg tcgagc
//
LOCUS       DBNGSX010000493         7449 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_673580, whole genome shotgun sequence.
ACCESSION   DBNGSX010000493 DBNGSX010000000
VERSION     DBNGSX010000493.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7449)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7449)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7449
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_673580"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..749
                     /locus_tag="AC16CB_14595"
     CDS             129..749
                     /locus_tag="AC16CB_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HIR3343748.1"
                     /translation="MDQRPIPDIVVGRLPIYLRSLQRMSQEGRLVTSSQEMSERLGIS
                     AAQIRKDLSQFGEFGKQGTGYNIEYLASQIKAILNVDRVWDMAVVGAGDIGSAVARYQ
                     GFANRGFQVTMIFDNDPEKIGTTIGPFTVQDYSRLSEIIPEAGIMVAMIAVPAFSAQD
                     VADELVKAGVKAILNYAPLNINVPEGIHVQHTDPVTHLQRMTYYLE"
     gene            complement(820..1827)
                     /gene="mreB"
                     /locus_tag="AC16CB_14600"
     CDS             complement(820..1827)
                     /gene="mreB"
                     /locus_tag="AC16CB_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HIR3343749.1"
                     /translation="MALLSRELGVDLGTMNTRVAEGGRVVLEEPTVAAIVVQEQKMVA
                     WGKEAMDMFGRVPESMEVTRPMQNGVIADYEVTEYMLQALLRRLSAPLRFFRPTIMVT
                     VPYGVTSVESRAVHEASLQAGSREAYLIQQPLAAAIGVDLPIGTPSGNMIVCLGGGAT
                     QAAVLAMYGIVSAETLRAGGMSLDEAIVSYIRKKYGLIIGQLTAEQVKIQIGAAVPQD
                     EESSFEIQGQDQVNGLPRPATLTTGEVVDAMRDPLNDIIETARRVLEKTPPELVSDII
                     DRGIALTGGGALLRGIDKLFTKQLGVPAYLVDNPTTCVAEGASRGLSIYPLIRRNLPP
                     V"
     gene            complement(1834..2286)
                     /gene="atpC"
                     /locus_tag="AC16CB_14605"
     CDS             complement(1834..2286)
                     /gene="atpC"
                     /locus_tag="AC16CB_14605"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HIR3343750.1"
                     /translation="MPIRCEIVSQDRLVFEGDADIVVVPGAMGEMGILPNHAPLLSTL
                     KFGILKVRYQGQEQVFAVAGGVVEIQPDIITVLADAAENVQEIDEARAEEARKRAEEY
                     LKAGPPPDTDAYLAMEAALRRSNLRLEAVQRYRRGQSVRQVQSGEGET"
     gene            complement(2296..3693)
                     /gene="atpD"
                     /locus_tag="AC16CB_14610"
     CDS             complement(2296..3693)
                     /gene="atpD"
                     /locus_tag="AC16CB_14610"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR3343751.1"
                     /translation="MTKGRVVQILGGVVDVEFPPEQLPEIFEAITIPRDGQNSLVLEV
                     QKHLGKNWVRCLAMDVTDGLQRGVAAYATGAPIKVPVGLETLGRVFNVLGQPVDNLGD
                     VEAKQYYPIHRPAPEFADQSTRVEVFETGLKVIDLIAPFTKGGKTGIFGGAGVGKTVI
                     IMELIRSIATVHQGNSVFAGVGERTREGTQLYREMNESGVMKDTVMVFGQMNEPPGVR
                     LRIALAALSMAEYFRDQGRDVLLFIDNIFRFSMSGSEVSALLGRMPSAVGYQPTLATE
                     MGELQERITSTRHGSITSMQAVYVPADDYSDPAPVATFTHLDATIALERSISEKGIYP
                     AVDPLASTSRILDPSLVGEDHYTTAREVQRVLQRYKDLQDIIAILGVDELSEDDKLIV
                     SRARKIERFFSQPMFVAAQFTGLEGRYVPLRDSVRGFREILDGKHDDLPEQAFMMVGT
                     IEEAREKAERLSQSS"
     gene            complement(3740..4639)
                     /gene="atpG"
                     /locus_tag="AC16CB_14615"
     CDS             complement(3740..4639)
                     /gene="atpG"
                     /locus_tag="AC16CB_14615"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HIR3343752.1"
                     /translation="MPNTREVRLRIRSIKNIAQVTRALQAVSASKVRKAMQAVMNTRP
                     YATKAWQVLTHIAGQPGRESLHPLLTNRPEVKSVLVLMITGDRGLAGAYNTNVVRFVL
                     HRFNDYPVPVRYITIGRKGRDLMLRRRKDVIAEFSNLPAAPAFADISAIGRIAVEEFL
                     HLHSDEVYLIYTDFISMARQVATIKKLLPLEVDTGEGRVMTFSQSARGPAAAYIYEPG
                     EAGIIDQIVPRFTALQVYQAILESLASEHAARMVAMKNATDSAKELAGALQLQYNKTR
                     QQAITNEMLDIAGGAEALESLRE"
     gene            complement(4702..6363)
                     /gene="atpA"
                     /locus_tag="AC16CB_14620"
     CDS             complement(4702..6363)
                     /gene="atpA"
                     /locus_tag="AC16CB_14620"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR3343753.1"
                     /translation="MSDLISQIAADLKKQIESFQPELEVRDVGSVIEAGDGIARVRGL
                     GDVRAQELVQFDNGVLGIAFNLESDQVGVIIMGEYAGIDEGMLVRSTGRIASVPVGDG
                     LVGRVVNALGQPIDGKGPIKYESFRPIERIAPGVVARKDVDTPVQTGLKAIDSMIPIG
                     RGQRELIIGDRQTGKTALAIDTIINQKGKDLICIYVAIGQKKAAIARTVATLERFGAM
                     DYTTIVVSAADEPAALQYIAPYAGCAMGEEFMETGRDALVVYDDLSKHAWAYRQVSLL
                     LRRPPGREAYPGDVFYLHSRLLERAARLADNFIIVKASHTDEVGDIKDSVNDTVYEGP
                     LAQHNAEAALKSMENADANKMVKLRGSGGSLTALPIIETLLGDVSAYIPTNVISITDG
                     QIYLENNLFYAGIRPAVNVGISVSRVGGDAQTRGMKQVAGRLRLDMASFRELAAFAQF
                     GSDLDKSTQAQLNRGQHLQEILKQPQYEPMSMESQVMVIFAGTQGYADKVSLERMRAW
                     ETSLLYFLETSYPEIGKDIAEKKRITDENMEKLRSALDAFNSTWQ"
     gene            complement(6561..7373)
                     /locus_tag="AC16CB_14625"
     CDS             complement(6561..7373)
                     /locus_tag="AC16CB_14625"
                     /inference="COORDINATES: protein motif:HMM:NF014068.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HIR3343754.1"
                     /translation="MIKTLLLFLILILTIGACSPRVDSNGINPFQDKPVAPLDVESSN
                     QTTHGIPTIVDLPGEQSKTTPTYPYPPLSPTATPKQEIDDPSLGKVLLMFGQRYDYRQ
                     YEGVCHALEEANYRIVIASNTLEQLEAMYIQHGFESSAPISRTLPLITADLLLGDVQI
                     SDYDAFILISDEALVFKGGEEVKQIIREAIEQGKVVAAHDAGVLILGNAGILQGVEIT
                     SNPLICMQLEEEDGAICRQRTVHRDGQIITAGPDHASSSFVKQIIAAIQDSN"
BASE COUNT         1823 a         2004 c         1755 g         1867 t
ORIGIN      
        1 aaggccaaac tcgagccatc ctttcctcat taactgcgta aatcctaatt attgattcct
       61 tgtgtgggtt tgcacaattg gataatttgt agtaatattt gatatcattc cacgggcagg
      121 agattaatat ggaccagaga cccatccccg atattgtggt cggacgcctt cctatttact
      181 tgcgatcctt acaacgcatg tcacaagaag gtcgtctggt aacatcgtca caagaaatga
      241 gtgagcgctt ggggatctcg gctgctcaaa tccgcaaaga cctatctcaa tttggggaat
      301 tcggcaaaca aggcaccggc tacaatatcg agtatttagc cagccagatt aaggcaattt
      361 tgaatgttga tcgagtatgg gacatggcag ttgtaggtgc tggggatatc gggagtgccg
      421 tggcgcgtta tcaaggattt gctaaccggg gatttcaagt aactatgata tttgataatg
      481 atcctgagaa aattggtacc acaatcggac ctttcacagt tcaggattat tcccgtttga
      541 gtgagattat tccggaagct ggcatcatgg tagcaatgat tgcggtccca gcattcagcg
      601 cacaagatgt agccgatgaa ttggttaaag cgggtgtcaa agcgattttg aactacgccc
      661 ctctcaatat taacgttccc gaagggatcc acgttcaaca caccgatccg gtgacgcacc
      721 tgcaacgtat gacttactac ttggaataaa tctccgtttg aaaagcaaga gagtagccgg
      781 atcggctact ctctttttcg ttaatgtaag cttataatcc tacacaggag gtaaattgcg
      841 gcgaatgagg gggtaaatgc ttaatccacg ggatgcacct tctgctacgc atgtcgtggg
      901 attatccacc aagtaagcag gaacgcccag ttgttttgtg aacaatttat caataccacg
      961 cagcagcgca ccacctccgg tgagggctat cccgcggtca atgatgtctg ataccaactc
     1021 agggggggtt ttctccaaaa cccgccttgc tgtctcaatg atatcattga gcggatcgcg
     1081 catggcatca acgacctcac cagtcgtaag tgtagctggg cgaggcagtc catttacctg
     1141 atcctgtccc tgaatttcaa aactgctttc ttcgtcctgg ggtacggctg ctccaatttg
     1201 aattttcacc tgttcagcag tgagctgtcc aatgatcagc ccatatttct tgcgaatata
     1261 cgacacaatc gcctcgtcta gcgacattcc acccgcacga agcgtttctg cggaaacgat
     1321 accgtacatc gccaacacag ctgcctgggt ggctccccct cccagacaga caatcatatt
     1381 accagatggg gtgccgatag gcagatccac cccaatggct gcagccaaag gctgctggat
     1441 aaggtaagct tcccggctgc ctgcctgaag agatgcctcg tgtaccgctc gactctccac
     1501 gctggtcacc ccataaggta cagtaaccat gatggtaggg cgaaaaaaac gcaggggtgc
     1561 gctcaagcgg cgcagcaagg cttgtaacat gtactcagtg acctcgtagt ccgcgatcac
     1621 accattttgc attggccgtg tcacctccat agattccgga acacgaccaa acatgtccat
     1681 tgcctcttta ccccaagcca ccatcttctg ttcttggact acaatcgctg caaccgtggg
     1741 ttcttcgagc acaacgcgtc caccctcggc cacacgggta ttcatggtgc ccaggtcgac
     1801 gcctagttct ctcgacagga gagccataat atattaggtt tctccctctc cagattggac
     1861 ctgacgaact gattgacctc ggcggtagcg ttgcactgcc tccaggcgca aattggaccg
     1921 gcgcagcgcg gcctccatag ccaggtaggc atcggtatca gggggtggac cggcttttag
     1981 atactcttca gcgcgtttac gggcttcttc agcccgcgcc tcatcaatct cttgcacatt
     2041 ctctgcagcg tctgccaaca cggtgatgat atcaggttgt atttccacca cacccccagc
     2101 aaccgcgaaa acctgttcct gcccctgata tcgtaccttg aggattccaa atttcagtgt
     2161 agatagcaag ggtgcatgat tcggcagtat acccatctcc cccatggcgc ctggtactac
     2221 aacgatatcc gcatcccctt cgaaaaccag gcgatcttga gagacgatct cacagcgaat
     2281 aggcatctta ttcctctagc tggactgaga taaacgctca gccttctcgc gcgcttcttc
     2341 gatggttccc accatcataa aagcttgctc aggtaggtca tcatgcttgc catccaagat
     2401 ttcacggaag ccacgcacac tgtctcttag tggcacatac cggccttcaa gtccggtgaa
     2461 ctgggcagcg acaaacatgg gttgcgagaa gaaacgctca atcttacgag cccttgaaac
     2521 aatcaacttg tcatcttcgc ttagctcgtc tacgcccaag atggcgatga tgtcctgaag
     2581 gtctttataa cgttgcagca ctcgttgtac ttcacgtgct gtcgtgtaat gatcttcacc
     2641 aaccagactt ggatcaagga tgcgggatgt tgatgcaagc ggatctaccg cagggtaaat
     2701 gcctttctca gagatcgatc gctccaaggc aatggtggcg tctaggtgag tgaatgtagc
     2761 aacgggagcc gggtcagaat agtcatcggc gggcacatac actgcttgca tcgacgtgat
     2821 cgagccatgg cgtgtagagg tgatacgctc ttgcagctca cccatctcgg ttgccagagt
     2881 gggttggtaa cccaccgcag agggcatacg ccctaacaat gcggatacct ccgagcccga
     2941 catgctaaag cggaaaatat tatcaataaa cagcagcaca tcccgtccct gatcacgaaa
     3001 gtactccgcc atggatagcg ctgccagggc aatgcgcagg cgcacccccg gaggctcgtt
     3061 catctggcca aataccatta cggtatcttt cataacgcca gattcgttca tctctcggta
     3121 gagctgggta ccctcgcgcg tgcgctcacc gacaccagca aaaacggagt tgccctggtg
     3181 tactgtagca attgaacgga tcaattccat aataatgacg gttttgccca cgccagcgcc
     3241 gccaaagata cctgtcttac cacctttagt aaacggggca atcaagtcaa taaccttcaa
     3301 accggtctcg aacacctcga ctcgtgtaga ttgatcagca aattcgggtg cggggcgatg
     3361 gatgggataa tattgcttag cctccacgtc gcccagatta tcgacaggtt gaccaagtac
     3421 attaaagacg cgcccaagcg tttcgagccc tacaggcact ttgatcggtg cgccagtggc
     3481 ataggcggcg acacctcgtt gaaggccatc ggttacgtcc attgccaggc aacgcaccca
     3541 gtttttcccc aggtgcttct gcacctccaa aacaagggag ttttggccgt cacgcgggat
     3601 tgtgatcgct tcaaaaattt cggggagttg ttcgggcgga aattccacat ccaccacacc
     3661 acctaaaatt tgaacgactc tacccttggt cattgttttt tctgctaaag ccatagcgcc
     3721 tatctccgtt ctctattcat tattctctca ggctttccaa tgcttccgca ccaccagcaa
     3781 tatccagcat ttcattggta atcgcctgtt ggcgtgtttt gttatattgc aattgaagcg
     3841 cacccgccag ctctttggcg ctgtcggtag catttttcat cgccaccatg cgggctgcat
     3901 gctcgctagc cagggattct aaaatcgcct gatatacctg caaagctgta aatctcggca
     3961 caatctggtc tataatgcct gcctcgcccg gctcataaat atatgctgca gctggtccgc
     4021 gcgcagattg ggaaaaggtc attacccgtc cttctccggt atccacttca aggggcagca
     4081 atttcttaat cgttgctact tgccgagcca tactgatgaa gtcggtatag atcaggtaca
     4141 cctcgtcact gtgcagatga agaaactcct caactgctat ccggccaatt gcggatatat
     4201 ccgcaaacgc aggagctgcc ggcaaattgc taaattcggc gattacgtct ttgcgccgac
     4261 ggagcatcaa atcacggcct ttacgtccaa tggtgatata gcggactggt acaggataat
     4321 cattgaaacg gtggagtaca aatcgtacga cgttggtatt gtatgcacct gccagaccgc
     4381 gatcacctgt aatcatcaac accaagacgc tctttacctc agggcgattg gttaataacg
     4441 gatgcaggga ttcgcgccca ggctgccctg caatatgcgt taatacctgc caagccttgg
     4501 ttgcataagg gcgcgtgttc atcactgcct gcatcgcctt gcggacttta ctggcgctca
     4561 ccgcctgcaa agcccgggtt acttgggcaa tattctttat gctccggatg cggagccgga
     4621 cctcacgagt attaggcata gatttctacc gagatcgtta tatccactct attaatcgtc
     4681 tgaaccagaa ccttcaatca attattgcca agtgctgttg aaggcatcca acgcactgcg
     4741 caacttctcc atgttttcgt cggtgatacg cttcttctcg gcaatatcct tgcctatctc
     4801 aggataggaa gtctccaaga aatataaaag cgaggtctcc caggcacgca tgcgctctaa
     4861 cgacacttta tctgcgtagc cctgtgtacc cgcaaagatg accatcacct ggctttccat
     4921 cgacatcggc tcgtactgag gttgtttgag gatttcctgc aagtgctgtc cacgatttaa
     4981 ctgtgcctgg gtggatttat ccaggtctga tccgaactga gcaaaagccg ccagctcgcg
     5041 aaatgaagcc atatccagtc gcaggcgccc tgcgacctgc ttcataccgc gtgtctgggc
     5101 atcaccacct acgcgggata ccgaaatacc cacattcaca gccggacgta taccggcgta
     5161 aaacaggtta ttctccaggt agatctggcc atccgtaatt gaaattacat tggtcgggat
     5221 atatgccgaa acatcgccca ggagggtctc aatgatggga agggctgtca atgagccgcc
     5281 tgagccacgc aatttgacca tcttatttgc gtctgcgttt tccatgcttt tcaaggcagc
     5341 ttcggcattg tgctgtgcaa gaggaccctc gtagacagta tcattgacgc tgtccttgat
     5401 atcacctact tcatcagtgt gactggcttt tactataatg aaattgtcgg caagacgagc
     5461 agcgcgttcc agcaagcgag agtgcaggta aaacacatca cctgggtagg cctcgcgccc
     5521 gggcgggcgg cgcagtaaga gcgaaacttg ccggtaagcc caggcatgtt tagagaggtc
     5581 atcatagaca accagagcat cacgcccggt ttccatgaat tcctcaccca tggcgcatcc
     5641 agcgtaaggg gcaatatact gtagagctgc tggttcgtcg gcagcggaaa ctacgatcgt
     5701 ggtgtagtcc atcgccccaa accgctcaag tgtggcgacc gtgcgggcga tggcggcttt
     5761 cttctgtcca atggcaacat agatacagat caagtccttg cctttttgat tgatgatcgt
     5821 atcaattgct agggcggttt tcccagtttg ccgatcgccg atgatcagct cgcgctgacc
     5881 gcgtccgatg gggatcattg agtctatagc ctttaaaccg gtctggacag gcgtatcaac
     5941 gtcttttcga gctaccacac cgggcgcgat gcgctcaatg ggacgaaagc tctcatactt
     6001 tatcgggcct ttgccatcaa tcggttgacc caaggcatta accacccgcc ctaccaggcc
     6061 atcacctacg ggcaccgatg caatgcgccc tgtcgagcgc accagcatcc cttcatcgat
     6121 accagcgtat tcgcccatga tgatgacacc cacctggtca ctttccaagt taaatgcgat
     6181 tcccaaaacg ccgttgtcaa actgaaccaa ctcctgggcg cgcacgtcac ccaaaccgcg
     6241 cacccgggcg atgccatctc cggcttcgat caccgagcca acatcacgta cctctaattc
     6301 aggttggaaa gattcaattt gtttttttag gtcagcggct atttggctga ttaaatcgga
     6361 catttcgcct ccgcagaata taatgattat acataccgcc gcccggataa atttaacatc
     6421 cgggcagcta ttggtttctg ataatggatt tcgtcagacc acaacccatg tagaaataat
     6481 tcctgatcat taaagtggtc aaatgatacc tgaaacagcc cgacttgtcc agttattcac
     6541 aaaaatatat ttggctcgcc ctagttgctg tcttgaatag ctgcaattat ctgcttcaca
     6601 aaacttgagc ttgcgtgatc cggcccggca gtgatgattt gtccatcccg atgtacagtc
     6661 ctttgcctac agatcgcgcc atcctcttct tccagctgca tacagataag tggattagaa
     6721 gtgatctcga ctccctggag aatgccagca ttgcctaaaa tcaaaacacc tgcatcgtgc
     6781 gcggccacaa cttttccttg ctcaatcgct tcacgtataa tttgtttgac ctcttcacca
     6841 cccttgaata ccaatgcttc atcactgatc aatataaatg catcatagtc actaatctga
     6901 acatccccca gcaaaagatc agcggttatt aacggtaaag tgcgtgaaat cggtgccgaa
     6961 gattcaaatc catgctggat atacattgct tcgagctgct cgagcgtgtt agaggcaatc
     7021 acaatccggt aattggcctc ctccagggca tggcatacac cctcatactg gcggtaatcg
     7081 tatcgctgtc cgaacatcaa taatactttt cctagagatg gatcgtctat ctcttgttta
     7141 ggtgtcgctg ttggcgataa aggcggatat ggataagtag gcgtggtttt tgattgctcg
     7201 ccaggcaaat ccactattgt tggaattcca tgtgtggttt gattggaaga ttctacatcc
     7261 aacggcgcca ccggtttatc ctgaaaaggg ttaattccat tggaatctac ccttggggaa
     7321 caagctccaa tcgttaggat caagatgagg aaaagtaaga gtgtttttat cattccaccc
     7381 acccttgaat atattgaatt attcggcagc tgatgatgta agtggcgcta tcggcatgcg
     7441 cgaggcatg
//
LOCUS       DBNGSX010000494         5004 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_603938, whole genome shotgun sequence.
ACCESSION   DBNGSX010000494 DBNGSX010000000
VERSION     DBNGSX010000494.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_603938"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="AC16CB_14630"
     CDS             <1..387
                     /locus_tag="AC16CB_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343755.1"
                     /translation="NSKNNRMVWGAMIAIATIAELVPDEIMPHIGRIKHLIETGTVIT
                     NVWGVKTLINLSKAGDRYYAALIGDLLDLQKQCRSVDFAKRAEDMSPVIQAAHLDEYK
                     AILVERKPSLSGAAQKRLQTVINRLG"
     gene            complement(401..763)
                     /locus_tag="AC16CB_14635"
     CDS             complement(401..763)
                     /locus_tag="AC16CB_14635"
                     /inference="COORDINATES: protein motif:HMM:NF039408.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6326 family protein"
                     /protein_id="HIR3343756.1"
                     /translation="MQDTRIILAGLWVALMLTYLWGDVLRIFAGDAEPGKIKGVEPTQ
                     ALWLFIAALMLIPIVMIVLTLTMKYPAIRWVNIIVAIFFVVFNIFGLPYKGAYDNFLI
                     LVSFVFNGLTVWYAWRWV"
     gene            complement(886..1839)
                     /locus_tag="AC16CB_14640"
     CDS             complement(886..1839)
                     /locus_tag="AC16CB_14640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIR3343757.1"
                     /translation="MKAIVCTKYGPPEVLQLKEVEKPIPKDNEVLIKIFATAVTASDG
                     IVRRFDLPRWHPLGLMMGLVVGFNKPRNPILGMVLAGEIESVGKDVTLFKSGDQVFGW
                     TLKSSLQTQFGTYAQYKCLPEDSVLILKPSNTSYEEAAAIPYGGLLALLYVTKGDIQS
                     GQKVLIYGASGAIGTLAVQLAKHFGGVVTGVCSTANIELVKSLGADAVIDYTKDDITK
                     RGERYDFILDAVGKRKSSDLKLQLKNVLTPNGKYVSVDEGSPNATVEDLIFLKELVEA
                     GQLKPVIDRCYPLEQIVEAHRYVDKGHKKGNVVITVDHNSQ"
     gene            complement(2298..2645)
                     /locus_tag="AC16CB_14645"
     CDS             complement(2298..2645)
                     /locus_tag="AC16CB_14645"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HIR3343758.1"
                     /translation="MSSKRRDRDYIADIQEAIQRILAYTQGMSWDTFLHDDRTQDAVV
                     RNIEVIGEAAKNLSGTLRKRYPHLPWKDMAGARDRLIHHYFGINNEIVWQIVQQDLPA
                     LLPKIQGILDELS"
     gene            complement(2638..2961)
                     /locus_tag="AC16CB_14650"
     CDS             complement(2638..2961)
                     /locus_tag="AC16CB_14650"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR3343759.1"
                     /translation="MNRPTTKEAVLQTLRRVFPSLREKYGVERLAIYGSFAKGSQGET
                     SDIDILVELSRPLGFGFIRLAADLEAALRRPVDLATFDSLKRSAAAPHRAHIARDIER
                     TLVDV"
     gene            complement(3045..3758)
                     /locus_tag="AC16CB_14655"
     CDS             complement(3045..3758)
                     /locus_tag="AC16CB_14655"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR3343760.1"
                     /translation="MHHSIEVENLSFAYPDGHTALRGVSLSIQPCEKVAVVGPNGAGK
                     STLILHLNGILIGQEGRIKVCDLEAAPGNLGRLRAMVGLVFQSPEDQLFSPTVYDDVA
                     YGPLYQGLSPQDVQARVDEALSAVHMSDYASRVSHHLSMGEKKRIAIATVLSMKPEVL
                     VLDEPTAGLDPRARRSLIKLLGELPLTMLVSTHDMLMVRELFPRMVIMDQGRVVADGP
                     TQVLLNDQALLEAHGLERP"
     gene            complement(3763..4563)
                     /gene="cbiQ"
                     /locus_tag="AC16CB_14660"
     CDS             complement(3763..4563)
                     /gene="cbiQ"
                     /locus_tag="AC16CB_14660"
                     /inference="COORDINATES: protein motif:HMM:NF014420.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HIR3343761.1"
                     /translation="MHVHFLDPYVSRTSPIHALDPRVKFVLTLAFILTVSLTPAQAWP
                     IYILLLSLILSIEILSNLGLRYVQKRAMLAFPFVLAAFPVIFTIPGTSLFSLSLGEWT
                     LVASQEGLARFAGIALKSWISVQAAIVLTATTPFPDLLVAMRAVRTPRLMVAVFGLMW
                     RYLFVLADEVLRLLRARSARSGELGIPGVKSGGSLAWRARVAGGMAGSLFLRAFERSD
                     RIYMAMLSRGYDGEVRALPLPPLKSWHLLALGIGLILDILFLLAALLI"
     gene            complement(4581..>5004)
                     /locus_tag="AC16CB_14665"
     CDS             complement(4581..>5004)
                     /locus_tag="AC16CB_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HIR3343762.1"
                     /translation="PAMAGVHALIGIGEGLITVGALAFIAAARKEMLKAGESQPAGGV
                     VWVFGLLAAIGLSVASPLASSNPDGLNWVAERYGFMGLEQGPLYNLIPNYIFPGISNG
                     ALASIAAGILGTLMVFGVALAVAILRRSKSGPKRLENP"
BASE COUNT         1118 a         1396 c         1423 g         1067 t
ORIGIN      
        1 aacagcaaga acaaccgcat ggtctggggg gcgatgatcg ccatcgccac catcgcagag
       61 cttgtcccag atgagatcat gccccacatc gggcgcatca agcatcttat cgaaacgggc
      121 acggtgatca ccaatgtctg gggggtaaag acgctgatca acctcagcaa agccggtgat
      181 cgatactatg ctgccctgat cggcgacctg ctggacttgc aaaagcagtg ccggagcgtg
      241 gattttgcga agcgcgccga agatatgtcg ccggtgatcc aggctgccca cctggatgaa
      301 tacaaagcca tcctggtgga gcggaaaccc agcctgagcg gcgcggctca aaaacggctg
      361 caaactgtga tcaatcgatt ggggtgaggg gaagggacgc ttagacccac ctccatgcat
      421 accacacggt caggccgtta aacacaaagc tgaccaggat gaggaaattg tcgtaagcgc
      481 ccttgtatgg caagccaaag atattgaaaa caacgaagaa tatggccacg atgatgttca
      541 cccagcggat ggcggggtat ttcatggtca gggtcaggac gatcatcacg atggggatca
      601 acattaaggc cgcgatgaac aaccacaacg cctgggtcgg ttcaaccccc ttgattttcc
      661 ccggctcagc atcgccggca aaaattcgca gcacatcgcc ccaaagatag gtcaacatga
      721 gcgccaccca taaccctgca agtatgatcc ttgtgtcctg catgattcag cctcctcctt
      781 tgtgaatgat tatgatcgac gttctattgc tgctgcttgt tgttatccct ggtggggcca
      841 tcacaagcct gaccctcttg cgtgatgttg aggcggtgcc gggtgtcact ggctgttgtg
      901 atccacagtg atgacgacat tccccttctt gtgccctttg tcgacgtacc tgtgcgcctc
      961 gacgatctgc tccagcgggt agcacctatc aatgaccggc tttagctgcc cggcttcaac
     1021 cagctccttg agaaagatca aatcttcaac cgtggcgttt ggactgccct catcgaccga
     1081 tacgtatttc ccgttggggg tcaatacatt cttgagctgc aattttaaat ctgagctctt
     1141 tctctttcca accgcatcca ggatgaaatc gtaacgctct ccccttttgg taatatcatc
     1201 tttcgtgtaa tcgatcactg catcggctcc cagggatttc accaattcga tattggcggt
     1261 gctacaaacc ccggtaacta cgcccccaaa gtgcttggct agctgtaccg ccagagtccc
     1321 gatcgccccg gaggccccat agataagcac tttttgcccg ctttggatat ccccttttgt
     1381 cacatacagc aaggctaaaa gcccgccata cggaatggct gcggcttcct cgtagcttgt
     1441 attggacggt ttcaatatga gcacgctgtc ttccggcagg catttgtact gggcatacgt
     1501 gccaaactgt gtttgaaggg atgatttgag ggtccatcca aaaacctggt cgccgctttt
     1561 gaataatgtg acatctttgc ctacggactc aatttctccc gccagcacca tcccgagtat
     1621 gggatttctc ggcttattaa agccgactac cagtcccatc atcagcccca gcgggtgcca
     1681 acgaggcagg tcaaatctcc tgacgatacc gtcgctggct gtcactgctg tcgcaaagat
     1741 cttgatgagc acctcgttgt ctttggggat gggtttttcc acctctttga gctgaaggac
     1801 ttcagggggg ccgtattttg tgcatacgat ggctttcatc gcactctcct tatttgacta
     1861 ttccgagatt ggccaaatct ttcacgtata gcataccgaa agtcggtatt cgtgtcatac
     1921 cccgaaggtg ttattcaggg ggttaattga taggttgatt ggaggaccaa aaaaccggac
     1981 tgctatctgt tgggagtgcg acgaagagcg cgatattatt ccctggacca ataaatggga
     2041 acctggctcg tgattctctg gtgggctagc accagtatac aaaattatct gtgtcagcgc
     2101 aacacaactt tgcggatatt tattttatgt cgcccccata ggactggcac tcggcgtgtc
     2161 gcagcaactg tgataaaagt caagggcaaa gatgaaatca ggcgggggat gcttaggctg
     2221 ttctgaaagg tattcgtctt ggcgctgtgt atctcccaat acggcactga gtacagggca
     2281 gccggtggta ataatcacta actcaattca tctaggatgc cttgaatttt aggcaacaag
     2341 gcgggcaagt cctgctggac gatctgccag acaatctcgt tgttaatccc aaagtagtga
     2401 tgaatgagcc gatcgcgcgc tccagccata tccttccagg ggaggtgcgg gtatctttta
     2461 cgaagggtgc ctgacaggtt cttggcagct tcgccgatca cttcgatgtt gcgcaccacg
     2521 gcatcttgtg tccgatcatc gtgaagaaag gtatcccagc tcatgccctg cgtataagcc
     2581 aggatacgct ggatggcttc ttggatatcg gcgatgtaat cccgatccct gcgcttacta
     2641 gacatccacg agcgtccttt cgatatccct ggcaatgtgg gcgcggtgag gagcggctgc
     2701 gctgcgtttg aggctgtcga aggttgcaag gtctaccggc cgtcgcaggg ctgcttcgag
     2761 gtctgcagcc aggcggatga aaccgaatcc taatggccgg gagagctcaa ccagaatgtc
     2821 gatatcactg gtttcgcctt ggcttccttt cgcaaatgag ccgtatatag ccaggcgttc
     2881 aacgccatat ttttcgcgca aggagggaaa tactcgccgc aaagtttgta gaacagcttc
     2941 tttggtggtt ggtcggttca tggcgcacct cacgcaccat ttaaaaaaag atgagttcta
     3001 gtcatatatt acttcaagcc tggcttgaaa tcaagaaaaa atctctacgg cctctccaag
     3061 ccgtgcgctt ccagcagggc ctggtcgttc agcaacacct gcgtcggccc gtcggcgacg
     3121 acccgcccct ggtccatgat caccatgcgc gggaacagct cgcgcaccat gagcatatcg
     3181 tgggtggaga ccagcatggt cagtggcagc tcgcccagca gcttgatgag cgagcggcgc
     3241 gcccgcgggt ccagcccggc ggtgggctca tccaagacga gcacctcggg cttcatggat
     3301 agcacggtgg cgatggcgat gcgctttttc tctcccatgc tgaggtggtg ggaaacgcgg
     3361 ctggcgtagt cgctcatgtg caccgcgcta agcgcctcat ccacccgcgc ctgcacgtct
     3421 tgcggggaga ggccctggta gagcggcccg taggccacgt catcgtacac ggtgggcgaa
     3481 aacaactggt cttcgggcga ctggaatacc agccccacca tggcgcgcag ccgccccagg
     3541 ttgccaggcg cggcctccag gtcgcagact ttgatcctgc cttcctgccc gatgaggatg
     3601 ccgttgaggt gcaagatcag cgtggatttg cccgccccgt tgggcccgac cacggccacc
     3661 ttctcgcagg gctggatgga tagggatacg ccccgcagag ccgtgtgacc atcggggtat
     3721 gcaaaagaga ggttttcgac ttcgattgag tggtgcatgg ttttagatca ataaagcagc
     3781 taacaagaag aggatatcca atatgagacc gatccccagc gccagcaagt gccaggattt
     3841 caacgggggc aggggcaggg cgcgcacctc gccatcgtag ccgcgtgaga gcatggccat
     3901 gtagatgcgg tcgctgcgct cgaaggcgcg caggaacaag ctgcctgcca tccctcccgc
     3961 cacgcgcgct cgccaggcca ggctcccgcc gctcttgacc cccgggatgc ccagctcgcc
     4021 ggagcgcgcc gagcgcgccc gcagcaaacg cagtacctca tcggccagga cgaacaggta
     4081 gcgccacatc aagccgaaga ccgccaccat caggcgcggc gtgcgcacgg ctcgcatggc
     4141 gaccagcagg tccgggaaag gcgtggtagc ggtcagcaca atggcagcct ggaccgagat
     4201 ccaggatttc aaggcgatgc cggcaaagcg cgccagcccc tcctgggagg ctaccagggt
     4261 ccactcgcca agactcaggc tgaacagcga ggtgcccggg atggtgaaga tgaccgggaa
     4321 cgccgccagg acgaagggga aagccagcat ggctcgcttc tgcacgtagc gcaacccaag
     4381 gttggaaagg atctcaatcg acaggatgag cgaaagtaac aagatgtaga ttggccaggc
     4441 ctgggcagga gtcaaagaga ccgtcaggat aaaagccagg gtgagcacga acttgacgcg
     4501 tgggtctaga gcgtggatcg ggctggtacg ggaaacgtag ggatcgagga aatggacgtg
     4561 catggatcaa gaagggtgac ctacgggttt tcgagtctct tagggccaga tttgcttcgg
     4621 cggagaatcg ctactgccag ggcaacccca aataccatca acgtgcccag gatgcccgcg
     4681 gcgatgcttg ccagggcgcc attggagatg ccggggaaga tgtaattggg gatcaggttg
     4741 tacagcgggc cttgttccag tcccatgaag ccatatcgct ctgccaccca attcagcccg
     4801 tctgggttgc tggacgccag cggggaggca accgataagc cgatggcagc tagtaagccg
     4861 aagacccaca ctacgccccc ggctggctgt gattctccgg ccttgagcat ctccttccgt
     4921 gcggcggcga taaatgccag cgcgccaacc gtgatcaatc cctcgccgat gccgatgaga
     4981 gcgtgcacgc ccgccatagc tggg
//
LOCUS       DBNGSX010000495         4638 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1573383, whole genome shotgun sequence.
ACCESSION   DBNGSX010000495 DBNGSX010000000
VERSION     DBNGSX010000495.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1573383"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="AC16CB_14670"
     CDS             <1..835
                     /locus_tag="AC16CB_14670"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3343763.1"
                     /translation="LYRHMLGLDRPWPHRYLQWVGGIVTRFDFGWSLTFRVPVTEVIG
                     DRIIFTVVLSVVTIGLSYLIAIPLGIYSAMHQYSAGDYLFTVLGYLGMATPAFLFALI
                     LLYIAVIYLDTSAGGLFATEFQNAPWSWAKLLDFVKHMWMPVVVLGTSGAASTIRTMR
                     ATMLDEKNQLYVTVARAKGVPENKLIRKYPVRAALNPVISTIGWELATIVSGSPITAT
                     VLSLPDIGPIFLRALLNQDIYLSGTILLIYSSLTIVGTFLSDLLLAALDPRIRFGGGA
                     Q"
     gene            832..1986
                     /locus_tag="AC16CB_14675"
     CDS             832..1986
                     /locus_tag="AC16CB_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3343764.1"
                     /translation="MSALPDTAADAKAPILDIKIDERKKEAYYTATQFQLMWWKFRRH
                     RTAIIGSVVLGFFVFIMVFSEFLAPYGSQSRNSDYLFGNPQTPQFLDENGKFIGPFVY
                     GMTTSFDKDTMQLVLKEDTSKLHKVRFFAKGEPYKMWGLIKGDRHLFVVEDSYLHLFG
                     TDSLGRDVFSRTLFGTRTSLSIGLLGVFISFAVGLAAGGVSGYLGGPVDNFLQRTIEF
                     IRSIPTLPLWMSLAAILPKEWSPLRVYFAVTIILGFITWTTLARRVRGKLISMREEDF
                     VFAARIAGSSELRIIWRHMLPAFLSYIIVDLTISFPAMILGETTLSFIGLGLREPVVS
                     WGVLLQQAQNIKAIEQHPWLFIPAIFVVISVLAFSLMGDGMRDAADPYSQ"
     gene            1997..3016
                     /locus_tag="AC16CB_14680"
     CDS             1997..3016
                     /locus_tag="AC16CB_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3343765.1"
                     /translation="MPLDTDELIRISNLKVTFSLEEGTVQALDGIELSIKKGKVIGVV
                     GESGCGKSVTARSILRIIPPPGKIDEGEILLRTRIGVPENIEREIDLAKIDPDSDLMR
                     QIRWGEISMIFQEPMTSFSSYHTIGSQIGEAVLEHRKTTRKEASELTIHMLDRVGIPN
                     ASTRANQYPHEFSGGMRQRAMIAMALICNPSLLIADEPTTSLDVTIQAQMLELMRDLR
                     KDFGSSVMFITHNLGVIAQIADEVYIMYLGKIVEHGTVADIFHNPKHPYTVDLLHAIP
                     KITGKAKERLVAIEGGIPSPFERVGGCPFHPRCNQAIPGVCEVQVPVMTDFGKGHTAF
                     CHLYP"
     gene            3056..4075
                     /locus_tag="AC16CB_14685"
     CDS             3056..4075
                     /locus_tag="AC16CB_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3343766.1"
                     /translation="MADERQKLIEVRNLRKYFPIRRGAFGASKKVVKAVDDISFFIYR
                     GETFGLVGESGSGKTTTGRLVLRAYEPTSGSTIYHLAGQEPMDVAKLTKKELWQFRKH
                     AQMIFQDPYSSLNPRMTVRDIIAEPLVATNSARGEQVTKRVSETARRCQLSIEHLRRY
                     PHAFSGGQRQRIGIARALVLRPDFIVCDEPVSALDVSIQAQILNLLKDLQAELNLTYL
                     FIAHDLSVVEHICNRVGVMYLGNLFEMADTQELFHHPLHPYTEALMSAIPEADPSIVM
                     QPIKLTGEIPSPIDPPSGCSFHPRCQYAQEVCSTTKPEWVEQQPEHFVACHFADSLQL
                     KGFAG"
     gene            complement(4087..>4638)
                     /locus_tag="AC16CB_14690"
     CDS             complement(4087..>4638)
                     /locus_tag="AC16CB_14690"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR3343767.1"
                     /translation="SDYTASELSSLNAACRFPDWSNREGVPSLQNVLERYHAGIHRWQ
                     LEIKTDAPKRLEVVCKILARQIDHFDIYERVKVTSFDPAALQIMRRTAPHLSLGLIRS
                     PMLLEDIDLAQTLGCSEVCMPVESGGAWLVSAAHEAGLEVTGWLGNNPTQMERLLAWG
                     VDAITTDVPSFAQQYLNNRSNLE"
BASE COUNT         1007 a         1342 c         1212 g         1077 t
ORIGIN      
        1 cctctaccgg catatgctgg gcctcgaccg cccctggccg caccgttatt tacaatgggt
       61 cggcggtatc gtcacccgct ttgattttgg ctggtccttg accttccgcg tcccggtgac
      121 cgaagtcatc ggcgaccgga tcatctttac ggtagtgctc tcggtggtaa ccatcggact
      181 ttcttatctc atcgccatac cccttggaat atattctgcc atgcaccagt attccgccgg
      241 agattacctg ttcactgtgc ttggatatct tggcatggct accccagcat ttttatttgc
      301 attgatcctg ttatatattg ccgtcatata cctggacacc agtgctggcg gattatttgc
      361 caccgaattc caaaacgcac cctggtcctg ggcgaaattg ctggattttg tcaaacatat
      421 gtggatgccg gtagtcgtat taggcacatc tggtgcagcc tccaccatcc gcaccatgcg
      481 cgccaccatg ctggatgaaa agaaccagct ttacgttaca gttgccaggg ccaaaggtgt
      541 cccggaaaat aaactcatcc gaaaataccc ggtgcgggct gcgctaaatc ctgtgattag
      601 caccattggc tgggagttag ccacgattgt atcgggatcg cccattactg ccactgtttt
      661 atctttgccc gatatcgggc ccatcttttt gagagcgctc ctcaaccagg atatttacct
      721 ttcgggcacc atcctgttga tctattccag cctcaccatc gtcggtacgt ttttatctga
      781 tttacttctg gcggctcttg atccccgcat ccgctttggt ggaggtgccc aatgagcgct
      841 ttacctgaca ctgccgccga cgcgaaagcg ccgatcctgg atatcaagat cgacgagcgg
      901 aaaaaggaag cctattacac cgccacccag ttccagctga tgtggtggaa gtttcgccgg
      961 catcgcaccg ctatcattgg ctcggtggtg ctcggattct tcgtgttcat catggtattc
     1021 tctgagttcc tggcacccta tggatcgcaa tctcgaaact ctgattatct gtttggtaat
     1081 ccccaaacgc cccagttcct tgatgaaaac ggcaaattca ttggcccgtt tgtttacggg
     1141 atgacgacca gttttgataa ggatacgatg cagcttgttc tcaaagagga tacttccaag
     1201 ctccacaagg tacgcttttt cgctaaggga gagccatata agatgtgggg gctcattaaa
     1261 ggcgaccggc acctgtttgt cgttgaggac agctacctcc acctgttcgg gacggacagc
     1321 ctgggtagag atgtcttctc caggacgctc tttgggacgc gcacatccct gtcgatcggt
     1381 ttgcttgggg ttttcatctc ttttgcagtg ggcctggcgg cgggaggcgt ctccggttac
     1441 ctgggcggcc ctgttgataa tttcctacag cgcacgatcg aatttatccg ttcgatcccc
     1501 accttgccgt tgtggatgtc actggcagcc atccttccca aggagtggtc gccattacgg
     1561 gtttattttg ccgtaaccat catcctggga tttatcacct ggaccacgct agcccgccgg
     1621 gtgcgcggca agctgatttc catgcgcgag gaggacttcg tcttcgccgc tcgcatcgct
     1681 ggcagcagcg aactgcgcat catctggcgc cacatgctcc cggcattttt gagctatatc
     1741 attgtcgacc tgacgatttc cttcccggcc atgatcctcg gcgaaaccac gctcagcttc
     1801 atcggcctgg gtttgcgcga gcccgttgtc tcctggggag tgcttctaca gcaagcgcag
     1861 aacatcaagg ccatagagca acacccctgg ctgttcatcc cggccatctt tgtggtgatc
     1921 agtgtgctgg ctttcagcct gatgggggac ggcatgcgcg atgccgcaga tccatattct
     1981 caataagagg tatgttatgc cactggacac agacgaactg atccgcatca gcaacctcaa
     2041 ggtaactttc tcgttggagg agggcaccgt ccaggcgctg gatgggattg agctgagcat
     2101 caaaaaaggg aaagtcatcg gcgtggttgg ggaaagcggt tgcggcaaga gcgtcacggc
     2161 acgcagtatt ctgagaatta tcccgccacc cggcaagatc gacgagggtg aaatcttgct
     2221 caggaccagg attggcgtgc ctgagaacat cgaacgcgag atcgacctgg ctaaaattga
     2281 tcccgacagc gacctgatgc gccagatccg ctggggcgaa atttcgatga tcttccagga
     2341 acccatgacc tccttctcct cgtaccatac catcggaagc cagatcggcg aagctgtcct
     2401 ggagcaccgt aaaaccacaa gaaaggaagc cagcgagctc accatccaca tgctggatcg
     2461 tgtgggcatc ccgaacgcct caacccgcgc caaccagtac ccccacgaat tttctggcgg
     2521 catgcgccag cgggcgatga tcgccatggc gttgatctgc aatccctcct tgctgatcgc
     2581 agacgagcca accacctccc tggatgtgac catccaggcg caaatgctcg agctgatgag
     2641 ggacctgcgc aaggacttcg gctcgagcgt catgttcatc acccacaatc tgggggtgat
     2701 tgcccagatc gctgacgaag tctacatcat gtacctgggg aagatcgttg agcacgggac
     2761 cgtcgccgat atctttcaca acccgaaaca cccgtatacg gtggacctgc tgcacgccat
     2821 ccccaagatc accggcaaag ccaaagagcg gctggttgcc atcgagggcg gcatccccag
     2881 ccccttcgag cgagtgggag gttgcccttt ccatccgcgt tgcaaccagg ccatcccggg
     2941 cgtttgcgaa gtccaggtac cggtgatgac cgactttggc aaggggcata cagctttctg
     3001 ccacctttat ccataaccgg gcagcttccc tcatcgaacc tctgggagtg tatgaatggc
     3061 tgacgaaaga caaaaactca tcgaagttag aaacttgcgc aaatatttcc ccatccgccg
     3121 cggcgctttt ggcgcttcca aaaaggtggt caaagcggtg gatgatatca gctttttcat
     3181 ctatcgaggc gagacctttg ggttggtggg agagagtggt tccggcaaga ccaccaccgg
     3241 ccggctggta ttgcgggctt atgaacccac ctctggctcg acaatatatc acctggccgg
     3301 gcaggagcct atggacgtgg cgaaattgac caaaaaagag ctctggcagt ttcgtaaaca
     3361 cgcccagatg atcttccagg acccttactc ctcactcaat ccacgcatga cggtgcggga
     3421 tatcatcgcc gaaccgctgg tagccaccaa ctcagccagg ggagagcagg tcaccaaacg
     3481 cgtcagcgag actgccaggc gctgccagct cagcatcgag caccttcggc gctacccgca
     3541 cgccttcagc ggcggccagc gccagcgcat tggcattgcc cgcgccctgg tgttacgacc
     3601 agatttcatc gtttgcgacg agccggtatc ggcgttggat gtatccatac aggcgcaaat
     3661 actcaacctg ctgaaagacc tgcaagccga attgaacctg acttacctgt tcatcgccca
     3721 cgacctgagc gtggtggaac acatctgcaa ccgggtgggt gtgatgtacc tggggaacct
     3781 gttcgaaatg gcagatacac aggaactgtt ccaccatccc ctgcacccct acaccgaagc
     3841 cctcatgtcc gctattcccg aggctgaccc cagtattgtg atgcagccta ttaagctcac
     3901 tggtgaaata cccagcccta ttgatccacc aagcggctgt tccttccatc cccgctgcca
     3961 gtatgcccag gaggtctgct caacgaccaa gccggagtgg gtagagcagc agccggagca
     4021 tttcgtcgcc tgccacttcg ccgattctct gcagctcaag ggatttgcag gctgatctca
     4081 tctcctttat tctaaattcg atcgattgtt cagatattgc tgtgcaaatg agggcacatc
     4141 tgttgttatg gcatctacgc cccaagccag taagcgttcc atctgtgttg ggttgttgcc
     4201 tagccagccg gttacctcca agcccgcctc gtgagcagcg gaaaccagcc aagcaccgcc
     4261 gctttccaca ggcatacata cttctgaaca acccagggtt tgcgcgagat caatatcttc
     4321 taatagcatc gggctgcgga tcagacctag gcttagatga ggagcagtac gacgcatgat
     4381 ttggagcgct gccgggtcga atgaagtcac tttaaccctc tcgtagatat caaaatgatc
     4441 gatttgccgg gcaagaatct tgcaaacgac ctccaggcgc ttgggagcgt cggtcttgat
     4501 ctccaactgc cagcggtgta tgcctgcatg gtagcgctcc aggacgtttt gcagagaagg
     4561 cacaccctcc cgatttgacc aatcgggaaa gcggcaggca gcgttcaggg aagataattc
     4621 cgaggcggta tagtcact
//
LOCUS       DBNGSX010000496         7134 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_674008, whole genome shotgun sequence.
ACCESSION   DBNGSX010000496 DBNGSX010000000
VERSION     DBNGSX010000496.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7134)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7134)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7134
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_674008"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="AC16CB_14695"
     CDS             <1..574
                     /locus_tag="AC16CB_14695"
                     /inference="COORDINATES: protein motif:HMM:NF024777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HIR3343768.1"
                     /translation="RDGSYSGVALGEQGIGDGHTCPFFDGASAYCNVYSTDLRDVFNG
                     SEGTAMIWAKPGVGALTDGAAHDFVRLQVDTANRVCIQKTATHNNFALWWEGADVLEI
                     VSLTVSTYVWHHFAITWSKSADQVKVYVDGAQSGSTQTGLNTWTGNLALAILGASSSA
                     PAFPWNGWLAHALLLDRAASAMEVAKCATV"
     gene            600..1259
                     /locus_tag="AC16CB_14700"
     CDS             600..1259
                     /locus_tag="AC16CB_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343769.1"
                     /translation="MTTLATFRSRIRAMLIDASGTIYADATVDEALRQALAEYNNALP
                     LERETVLILPGDGREIALNGIADLRAVTDVYWPFDSLDEPWPPNQVKGFYVFWDDTQP
                     VLYLNQIDGDGPQQDDELRLWYATNHTIDGLDSATETTIPKEHTSLLVAGAAGHAALM
                     RTVDLTEVSSADQYMTGLLGVWAQRVLKEFRAALEAIKRWKARAGAPYTQGWKLDKWD
                     D"
     gene            1264..1668
                     /locus_tag="AC16CB_14705"
     CDS             1264..1668
                     /locus_tag="AC16CB_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343770.1"
                     /translation="MMFDDFEKYFKELSPDAFKRRIPIILYRKGGDRTDWTTPGGQVF
                     APVSTIVMIGCVEWTGASATSGDVVQAFPVHYYGEPLIFIQPRVTTPSGAFLMVYALS
                     AGDAVEIYWKSDVAVTMARFAWIAFGGQLVSP"
     gene            complement(1669..2280)
                     /locus_tag="AC16CB_14710"
     CDS             complement(1669..2280)
                     /locus_tag="AC16CB_14710"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR3343771.1"
                     /translation="MFTRTIAMNQRIDGLTLVSLALILFMGFNVLRDRASTAHASSQL
                     ATGSQTAPSLPASTAQPTEAEMLEISAPYEDYILTQGPHGTSYGHKAIDLAAGKGEAV
                     LSPINGIVTERYVDEYDNPTLVIENDIYRVTLLHGKYTVSVGDRLQIGQPVGKESNLG
                     YTADIQGRPCNGRKKCGYHTHLNIYDKRLQANVNPLDLIGAAP"
     gene            2334..2657
                     /locus_tag="AC16CB_14715"
     CDS             2334..2657
                     /locus_tag="AC16CB_14715"
                     /inference="COORDINATES: protein motif:HMM:NF053320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF8156 family protein"
                     /protein_id="HIR3343772.1"
                     /translation="MGRTLPSITQVFLQEQESLSRFRRALRRSDQLALDDLFAAARQH
                     LAAAAYAGHALPFEVFLLAMLLEEHKMVLRLRSALEEMQAPGYTPASPPEQIGPQAEL
                     FKLKP"
     gene            2664..4865
                     /locus_tag="AC16CB_14720"
     CDS             2664..4865
                     /locus_tag="AC16CB_14720"
                     /inference="COORDINATES: protein motif:HMM:NF012364.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HIR3343773.1"
                     /translation="MEFSGWLLDVYADPHDGVALWLLDERDGSRRRLRQALPVAFHAA
                     GTAARLRQLWRYLQSEGLNGRVPVTLSRSERRELFSDQPVSVLTVQVSQPAAQPALFR
                     KVSQAFPDLTYYDADLSPALHHAARYGAFPLARCCVDADEQGQVQSLEVLNTPWDLDP
                     CAPPLRLLSLEPDVDPFHAAPTHILARAQRACYRLALPGPHAQGKAGIRPLLVNLRAI
                     FMRHDPDLLLTTWGDTWLLPYLLELSEHSGLSLPLNRDPAFGPLYRPERTYFAYGQVI
                     YRGQQVHLFGRWHVDGHNAVLYHDYGMEGIFELARVTSLPVQAVARLSPGSGISAMQM
                     LTALREGVLVPWHKQQAERPKTARELLRSDQGGLVYQPVIGLHKDVAEIDFVSMYPSI
                     MARFNISPETVKKEPHLSVLPDSGDGLVPRTLQPLLDKRIALKLRLAELPAWHPRRNA
                     YKARASAHKWLLVTCFGYLGYKNARFGRIEAHEAVTAYGREMLLRAKEAAEDLGYTVL
                     HLYVDGMWVLKEGANKVVDFQPVLDEIAERTSLPVSLDGIYRWVAFLPSRVDKRVPVP
                     NRYFGVFQDGSLKIRGIESRRQDTPPWIAHLQLSMLDCLSQARSAGELPGCTPAIERV
                     LRKAYSDLRLGRVPLEGLLVSQKLSRELDEYRVPSPAARAAAQLVSVGKHLRPGQRVR
                     FLYTLGEPGVRAWDLPDPPNPKTIDLRYYRKLLVRAAETVLQPLGVDLVLG"
     gene            5078..5926
                     /locus_tag="AC16CB_14725"
     CDS             5078..5926
                     /locus_tag="AC16CB_14725"
                     /inference="COORDINATES: protein motif:HMM:NF016424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIR3343774.1"
                     /translation="MKYLNYFFSFFLFLVLTLPISTGVIAQGATPPVPSPLSEGSYVL
                     DQLGWLTPAHEETINTIVAKLDEENLAEIAVVTYDDCGPDSQSYRNTFFRTWGIGHKE
                     RNDGLLILVCWYGGDKSRRKLEQEVGYGMEGTIPDLLTSKVAEAHFIPVWKSGKDASR
                     AIADGDAGAALVAMVKAYDSVIRGDTPEELAQRPSSSSSSSDAGFQVIGWLLFAAILI
                     ILLVVWWKRIESGSDDGSSSDWGSSDDWSSSDRDSSGGWDSSDDSDRSSFGGGSSGGG
                     GSSSGF"
     gene            5983..6621
                     /locus_tag="AC16CB_14730"
     CDS             5983..6621
                     /locus_tag="AC16CB_14730"
                     /inference="COORDINATES: protein motif:HMM:NF015944.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR3343775.1"
                     /translation="MNLGNRTTLIVLAIVVFVIFVCGASIASTYNGMVRSANNVRMVW
                     ADVESAYQRRLDLVNQTLPSVVAGAQQELAVFKTLRDQAAALSASFKRDELGQPIVPQ
                     GADAQNIQQQIVAFDRAWINALAYMADNPEIQSTQLYSDFMIQVEGSENRINVARRDY
                     NAAVNDYRNTTQTFPNNLAAGIFGFDPNQFAYFQAQPGAEQAPEVSFPTPVP"
     gene            complement(6643..>7134)
                     /locus_tag="AC16CB_14735"
     CDS             complement(6643..>7134)
                     /locus_tag="AC16CB_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR3343776.1"
                     /translation="THQVNRVAALAASAAEMLAFGQQYMTQIVRNAQALGAALHKRGI
                     PVLGAHKGFTETQQVIADVGNFGGGLEVAQRLARANLIANKNLIPTDTPANWDRPSGL
                     RMGTIEVTRLGMREAEMEQIADFIARLLVDKAAPEDILEDVIDFRQPYQTLYYCFENG
                     LPG"
BASE COUNT         1446 a         1950 c         2108 g         1630 t
ORIGIN      
        1 ccgggatggc tcttacagcg gggttgccct gggcgagcaa ggcattggcg atggacatac
       61 ttgccccttc ttcgatgggg caagcgctta ctgcaatgtc tattccaccg acctgcggga
      121 tgtgttcaat ggatcggaag gcacggcgat gatctgggcc aagcccggtg taggcgcttt
      181 gacggatggg gcggcgcatg atttcgtcag gcttcaagta gacacggcaa atcgcgtgtg
      241 tattcaaaag acggcaacgc ataataactt cgccctatgg tgggaaggtg cagatgtgct
      301 tgagatcgtc agcctgacag tttccactta tgtgtggcat cactttgcca ttacatggtc
      361 gaaaagcgct gatcaggtga aagtctatgt cgacggcgct cagtctggtt caacgcaaac
      421 ggggctaaat acatggacag gcaacctcgc gcttgccatc ctcggggcca gctcttccgc
      481 ccccgccttt ccgtggaacg gctggcttgc ccatgccttg ctgctcgacc gcgccgccag
      541 cgcgatggag gtcgccaagt gcgccaccgt atgaggctat ctgcctgtgg gattaaccta
      601 tgaccacact tgccaccttt cgctctcgca tccgcgctat gttgatcgac gcttctggca
      661 ccatctacgc cgatgccacg gttgatgaag ccttacgcca ggccctggcg gagtacaaca
      721 atgccctgcc actcgagagg gagaccgttt tgatcttgcc gggcgacggg cgcgagatcg
      781 ctttgaatgg gatcgcagat ttgcgcgccg ttaccgatgt gtactggccg ttcgatagcc
      841 tagacgagcc ctggcctcct aaccaggtga agggtttcta tgtcttttgg gatgatacgc
      901 agccggtgct ctatctcaac cagatcgacg gcgacgggcc tcagcaagac gatgagctgc
      961 gcttgtggta cgccacaaac cataccatcg acggcctgga ttccgccacc gagacgacaa
     1021 tacccaagga gcacacctcg ctgctggtgg cgggcgctgc aggccacgcg gcgctgatgc
     1081 gcacggttga tcttacggag gtctcttccg cggatcagta tatgaccggc ttgctgggcg
     1141 tatgggcgca gagggtgctc aaggagttcc gcgctgctct tgaggcaatc aagaggtgga
     1201 aggcacgcgc cggtgcccca tatacccagg gttggaagct cgataaatgg gacgattgat
     1261 cagatgatgt ttgacgactt cgaaaagtac ttcaaagagc tttcgccaga tgccttcaaa
     1321 cggcgtatcc cgattattct ctatcgtaag ggaggagatc gcacagattg gacaacgccg
     1381 ggcggccagg tgtttgcccc ggtttcgacg atcgtcatga tcggttgtgt tgaatggacg
     1441 ggcgcttctg caacgagtgg agatgttgtc caggcattcc cagtccatta ttatggcgag
     1501 ccattgatct tcatccagcc gcgggtgacc acgccatccg gcgcttttct gatggtgtac
     1561 gccctttcgg cgggtgatgc agtggagatt tattggaaat ccgatgtggc tgttacaatg
     1621 gcccgctttg cctggatcgc ttttggcgga cagctagttt cgccgtgatc atggggctgc
     1681 gccgatcagg tccagtgggt tgacattggc ctgcaggcgc ttatcgtaga tgttcagatg
     1741 cgtatggtaa ccacacttct tgcgtccatt acagggtctg ccttgtatat cggcggtgta
     1801 gcccagattg ctctccttac ccaccggctg gccgatctgc aatcgatcgc ctaccgagac
     1861 ggtgtatttg ccatgcagca gagtgacacg gtagatgtca ttctcgatca caagtgtcgg
     1921 gttgtcgtac tcatcaacgt agcgctcagt gacgattccg ttgatgggcg aaagcaccgc
     1981 ctctcccttg ccagcagcca ggtcgatcgc cttatggcca taggaggttc cgtgcggccc
     2041 ctgtgtgagg atgtagtcct cgtagggtgc ggaaatctcc agcatctctg cctcggtggg
     2101 ctgggcggtg gaagccggca aggacggtgc ggtctggctg cctgttgcaa gttgggagga
     2161 ggcgtgcgcc gtgctggctc gatctcgcag gacgttgaag cccatgaaca ggatcagtgc
     2221 cagggataca agggtgagac catcaatacg ctggttcata gctatggtgc gagtaaacat
     2281 ctccacaaaa tagaatattt gttctaatta taccatgaaa caggaggtat gctatgggac
     2341 gtacacttcc atcaattaca caagttttcc tgcaagagca agagtctttg tctcgtttcc
     2401 ggcgcgccct gcggcgcagt gaccaactgg cgctggacga cctgttcgcc gcagcacgcc
     2461 agcacctggc agctgcggcg tacgctggtc atgccctgcc tttcgaggtc ttcttgttgg
     2521 ccatgctatt agaggagcat aagatggtgc taagattgcg ctctgccctc gaggagatgc
     2581 aagcgccagg ctacaccccg gctagccctc cagagcagat aggcccacag gctgagctat
     2641 tcaagctaaa gccataggcg tcaatggaat tctctggctg gctgctggac gtatacgccg
     2701 atccacatga tggcgttgcg ctctggctac tggacgagcg cgatggctct cgccgccgcc
     2761 tgcggcaagc gctgccggtg gctttccacg ctgcaggtac agcagcgcgc ctgcgccaat
     2821 tatggcgcta cctgcaatca gaagggctga atggcagggt gcctgtaacc ctttcccgta
     2881 gcgagcgccg cgagctgttc tccgaccagc ctgtgtctgt gctcactgtg caggtgagcc
     2941 agccggctgc ccagccagct ttgttccgca aggtttctca ggcttttccc gacctgacct
     3001 attatgatgc tgacctttcc ccggctttgc accacgcggc tcgctacggt gctttccccc
     3061 tggcgcgttg ctgtgtggat gccgacgaac aggggcaggt gcaatccctg gaagtcctga
     3121 atacaccctg ggatttagat ccctgcgcac ctcctctgcg cctcctttcc ctggagccgg
     3181 atgtagaccc cttccatgct gccccaaccc acatcctggc gcgcgcgcaa cgagcctgct
     3241 accgcctggc gctccccgga ccgcatgcac agggcaaggc aggcatccgc ccattgctgg
     3301 tgaacctgcg tgccatcttc atgcggcacg accccgactt gctgctgacc acctggggcg
     3361 atacctggtt gctgccttac ctgctggagc tctcagagca cagcggcctg tccctgccgc
     3421 tcaaccgcga ccctgccttt ggaccactct accgcccgga gcgcacttac ttcgcctatg
     3481 ggcaggtgat ctaccgtggc cagcaagtgc acctgtttgg tcgctggcac gtggatgggc
     3541 acaacgctgt gctctaccac gattacggca tggagggcat ctttgagctg gcgcgcgtga
     3601 ccagcctgcc agtgcaggcg gtggcgcgtc tctcgccggg cagcggtatc tctgcgatgc
     3661 agatgctcac agccttgcgg gagggcgtgc tggtaccctg gcataagcaa caggctgagc
     3721 gccctaagac cgcccgggag ctgttgcgct ctgaccaggg aggcctggtg taccagccgg
     3781 tgatcggcct gcacaaagac gtggcggaga tcgacttcgt ctctatgtac cccagcatta
     3841 tggcgcgctt caatatttcg cccgagacgg tgaagaaaga acctcacctg tctgtccttc
     3901 ccgacagtgg ggatggattg gtgccaagga ccctgcaacc cctgctggac aagcgcattg
     3961 ccctcaagct gcgcctggca gagctgcccg cctggcaccc gcgccgcaat gcctacaagg
     4021 cgcgcgcctc agcgcacaag tggctactgg tgacctgttt cggctacttg gggtacaaga
     4081 acgcccgctt tgggcgcatc gaggcgcacg aggcggtgac tgcctacggg cgcgagatgt
     4141 tgctgcgtgc caaggaggct gccgaagacc tgggctacac ggtgctgcac ttgtatgtgg
     4201 atggcatgtg ggtgctgaaa gagggggcga ataaagtggt tgacttccag cctgtgctgg
     4261 acgagatcgc cgagcgcacc agcctgccgg tgtcactgga cggcatctac cgttgggtgg
     4321 cttttctgcc ttcgcgcgtg gacaagcgcg tgccagtgcc caaccgctac tttggggtgt
     4381 ttcaagatgg ctcgttgaag atacgcggca tcgaatctcg ccgccaagat acgccgccct
     4441 ggattgcaca cttgcaactg tctatgctgg attgcctgtc gcaggcaaga tcagccggtg
     4501 aactgccagg ttgcaccccg gcaattgagc gcgtgctgcg caaggcctat tccgatctgc
     4561 gcctggggcg cgtgccactg gagggcctgc tggtttcgca gaagctcagc cgggagctgg
     4621 atgagtaccg cgtcccttcc ccagcggcgc gtgcggcggc tcagctggta tcggtgggaa
     4681 agcatctcag gccggggcag cgcgtgcgtt ttctctacac cctgggagag ccgggcgtgc
     4741 gtgcctggga cctgcctgac ccacccaatc cgaagacaat cgacttacgc tattaccgga
     4801 aactgctggt gcgggcggca gagacggtgc tgcaaccgct cggggtggat ctagtattgg
     4861 gctaatcctt gccagaatag tggttgcaaa aatgcgattg actcgaagta aaatgaatat
     4921 ataattgttt atgagcttgg tctgttcgtg tttcagtggc taaaacaaga caatccgcat
     4981 tgtatagccg agagttaata aaaagtaacg atcggttttt gattctacgc aaatcgctgg
     5041 ttgtgagttg aatttgccaa ttggggagga ttgaaagatg aagtatctca attacttctt
     5101 tagtttcttc ttattcttgg tgctgacttt gccgatttcg actggtgtaa tcgcgcaggg
     5161 agccactcca ccagtcccat cacctctcag tgaaggttcc tatgttttag accagcttgg
     5221 ctggctgact ccagcacatg aggaaacgat caacacaatt gtggcaaagt tggacgaaga
     5281 gaatctggct gagatcgctg tggtgaccta cgacgattgt ggtcccgaca gccaatccta
     5341 ccgtaataca ttctttcgta cttggggtat cggtcacaaa gagcgcaacg acgggttgct
     5401 gatcctggtt tgctggtacg gcggtgacaa atcccgtcgc aagctggaac aagaggttgg
     5461 ctacggaatg gagggcacca tcccagactt gctgaccagt aaggtggccg aggctcattt
     5521 tatacctgtc tggaaatccg gcaaagatgc cagcagggca attgcagatg gcgatgccgg
     5581 agctgccctg gtggcgatgg tgaaggcata tgacagtgtg atccgcgggg atacgcctga
     5641 agagcttgca caacgcccta gtagcagctc atcaagctcc gatgctgggt ttcaagtgat
     5701 cgggtggtta ttattcgctg cgattcttat tatattgtta gtggtatggt ggaagcgtat
     5761 agaaagcggt tctgacgatg gtagctctag tgattggggt agctcggatg attggagttc
     5821 gagtgatcga gatagctcgg gtggttggga tagctctgat gattcagata gatcgtcttt
     5881 cggtggaggg tcatctggcg gaggcggctc gagttcgggt ttttagcaaa ccagtgaaag
     5941 atactccgcc gtgccattta aacatagaag gaggacaagc cggtgaatct aggaaatcga
     6001 acaactttaa tcgtgctggc cattgtcgtt ttcgttatat ttgtatgtgg agcatcaatc
     6061 gcatctacat acaatgggat ggtgcggtct gcgaacaatg tccggatggt gtgggcggat
     6121 gtggagtctg cttatcaacg tcgccttgac ttggtgaacc aaacattgcc cagcgtcgtt
     6181 gctggagccc agcaagagct ggctgtcttc aaaacccttc gagatcaagc tgcagcatta
     6241 tctgcctcat tcaaaaggga tgagcttggt caaccgattg tcccacaggg cgctgatgca
     6301 cagaacatcc aacaacagat tgtagcgttc gatcgcgcct ggattaatgc gttagcctac
     6361 atggctgata acccggaaat ccagtcgacc cagctctact cggactttat gatccaagtc
     6421 gaggggtctg aaaaccgaat taatgtggct cggcgtgact ataacgcggc ggtaaatgat
     6481 tatcgaaata ccacgcagac tttcccgaac aatctagctg ctgggatttt tggatttgat
     6541 ccaaaccagt ttgcctattt ccaggcacaa cccggcgctg agcaggctcc agaagtcagt
     6601 ttcccaacac ctgtgcccta gaaaaagtgc cttgttgtac tattagcctg gcaacccgtt
     6661 ttcgaagcaa tagtacaaag tctggtaggg ctggcgaaaa tcgatcacgt cttccaggat
     6721 atcctcgggg gcagccttat ccactaacag gcgggcgata aagtcggcga tctgttccat
     6781 ctcagcttcg cgcatcccca agcgggtgac ttcaatagtg cccatgcgca gaccgctggg
     6841 gcgatcccaa ttggcagggg tatccgtggg gatcaaattc ttattggcga tcaggttggc
     6901 acgcgccaag cgctgggcaa cttccagccc gccgccgaaa ttacccacgt cggcgatcac
     6961 ctgctgggtc tcggtaaaac ccttgtgcgc gcccaagaca ggaatgccgc gcttgtgcag
     7021 cgctgctccc agggcttggg cgttgcgcac gatctgggtc atgtactgtt ggccgaaagc
     7081 cagcatctct gcggcagatg ctgccagtgc tgccacccgg ttcacctggt gagt
//
LOCUS       DBNGSX010000497         7085 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1365172, whole genome shotgun sequence.
ACCESSION   DBNGSX010000497 DBNGSX010000000
VERSION     DBNGSX010000497.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7085)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7085)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7085
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1365172"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="AC16CB_14740"
     CDS             <1..280
                     /locus_tag="AC16CB_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343777.1"
                     /translation="LSEILVNPELRQRYYRELILPSIELLEAHLQVRQDQGQIRPLNI
                     PMSVRFLVALTNGLFFLDVLGDPLVQSQWDELSQLITSIFFDGVDPRP"
     gene            309..2555
                     /locus_tag="AC16CB_14745"
     CDS             309..2555
                     /locus_tag="AC16CB_14745"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3343778.1"
                     /translation="MFLKNLFRRKIRTALTVLGISIGVAAILGLGAMASGLEAGYGSM
                     LSGSKADLILSQPDSFDMSYSVVGENIGVQLAAMPEIHALSSMLEGFVQAENEPYFFV
                     FGYPEDSFILDRFQIVDGAALSSREARSAHGKPILLGSAAAEVLDKAVGDSMRLTGSV
                     YRIIGIYQTGDAFEDSGAILRMQDAQELLGKPRQVSVFYIQLKDPALRERFIKRVERQ
                     MPELELSGVQEFQDKQIMSDMFAGYVWVIGGLAILIGGVGMMNAQLMSVFERTREIGV
                     LRSVGWSRSRVLWMILAESMFVCLAGGVLGLGLGYLLLDVISRNTVIFGASTASIGAS
                     SLQQAFIVVVVMGLVAGLYPAWRASRLQPVEALRYEGGAASKARRLPVGGMAVQSLRQ
                     RVTRTLLTVMVIGITVGAIAALEGLLLSMKADMTAMFSSGGNQIMIRQADVSDTSLSA
                     IDERIGDKIAALSEVETVSGVMIAAVVLPDTGGFFIMLGYGPNEQAIRRFRIVDGEPL
                     KSNHQIILGKSMADAMKKNVGDTIDLSGARFRVVGIYESNISWEELGGVASLRDTQLF
                     IGRPRKVTMFAVSVRDPSQAEEVVEKINATFPEVHASLSGEFADQMPDFEASEGMMSG
                     ISVMAIFIGGVGVLNTMLMAVFERTREIGVLRSLGWGRSAILSMILRESLLLGLLGGG
                     AGIAIAFCLVQLASIAPIVGDYFGVTWQWQIFARAIGIAIMLGLLGGLYPAYRATRLQ
                     PVEALRYE"
     gene            2570..3757
                     /locus_tag="AC16CB_14750"
     CDS             2570..3757
                     /locus_tag="AC16CB_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR3343779.1"
                     /translation="MQKKSLSLLFISVLTGLVLVSGCDALGQRATPTPEAVVEEDFVP
                     LVNATGVVVPARFTTLSMSASGVVAEVLVSENDIVSAGEPLVRLEGKEDLEAAIAAAR
                     FEVAAAQHDLDLLYKDLDLRLANAQQVVVDAKQALRDAERRQYNVERAAKQVDIDQAR
                     ANLVLARDKLEKAVEDFEPYENKPEDNLTRAALLSAKASAEEQYDSSVRLLNNLLAGA
                     NDLDVAEANAEAEVAKAQLAVAEREVELLKVGPDPEDVKLAEGRLANAKVQLTSAEAA
                     LGDLELLAPFNGTVTELYIKQSEWISFGQSVLLLADLDHLRVETTDLNEIDVARIDEG
                     STTLITFDALPDVSTQGQVIRIAPKASAGSGVNYTVVVEMDEIPKSLRWGMTAFVDIE
                     VQE"
     gene            3769..4602
                     /locus_tag="AC16CB_14755"
     CDS             3769..4602
                     /locus_tag="AC16CB_14755"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006826924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3343780.1"
                     /translation="MAPEWIVETRDLTRIYGDGEEIHALDGVNLQIAPGEVVAVMGPS
                     GSGKSTLLNMIGALDKPTRGQVFVNGQDLAKIRNKDAFRAKTVGFMFQLHNLLPTMTA
                     RENVEVPMMGHRGLRTRHKRAKELLELVGLPDRMNHLPNQLSGGQRQRVAVARALAND
                     PPLVLADEPTGSLDTVAGMDLLKLLHELNHSQGTTFLVVTHDIAVARQTHRVVVMADG
                     KIVREDLIGSPLEEDLKMWRHSGLGRRILEEEMDVIQRLGIDESQARVLREIFTSANS
                     D"
     gene            complement(4599..5645)
                     /locus_tag="AC16CB_14760"
     CDS             complement(4599..5645)
                     /locus_tag="AC16CB_14760"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR3343781.1"
                     /translation="MITRRSFLKLGGAAAASLFTHPSSPYLLLPDDEPPRLAGKARVA
                     TGLVYRYSQASFQSERIGNLKRDQIVDIHEETLSPDGPNYNPRWYRLSKGYAHSGRLQ
                     RLDGAHFHRDVLEAVPETGQLGEISVPFTQSYRKMPGNQWAPLYRLYYQSIHWITGLD
                     SGPDGRTWYRLTDDLLHVHHYVPSFHVRPVASDELSPISPRVPPDDKRIAVSIADQTL
                     TAYENDQIVFTSKVSTGIPSEPPPGMLPTETPRGRFYVQTKMPSRHMGDGNLTDDIEA
                     YELPGVPWVCFFHKDGYAFHGTYWHDNFGRMMSHGCVNMRTQDALWLYRWTTPVAPAK
                     NWFTRQMGTRVDIT"
     gene            complement(5642..6715)
                     /locus_tag="AC16CB_14765"
     CDS             complement(5642..6715)
                     /locus_tag="AC16CB_14765"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR3343782.1"
                     /translation="MQNRLSRRGFLKMGGMAVAGIALGPVFPRLDEQDYGDIARITIQ
                     QVDVRSQPNDKSDIVGNRLRDQIVHIYEEVISPDGPAYNPLWYRVWGGYLHSAYLQRV
                     KVRHNEPLSAIPEAGQLCEVTVPYTIAYQYSSWEGWQPWDGTPLYYTTTHWITGIEAG
                     PDGEAWYELTSEIDAHLKYFVPTAHLRPISDEEISPISPDVPPEQKRIEVSIGRQTLT
                     AYEGDKVMLSTKVSTGIPSARRSPEDLPTATPTGRFRIYSKMPNKHMGFITGNPDALR
                     NGGFSLPGVPWTCFFAFPGGYAFHGTYWHNNFGIQMSHGCVNLRNEDALWLFRWSTPV
                     FKTPIESHTDWERRGNGTTVEIG"
BASE COUNT         1492 a         1736 c         2201 g         1656 t
ORIGIN      
        1 cttgtccgag atattggtca atcctgagct gcgccagcgc tattaccggg agctaattct
       61 gcccagcatt gaactgctcg aagcgcatct gcaagtgcgc caggatcagg ggcaaatccg
      121 cccgctgaac attcccatgt cggtgcgctt cttggttgcc ctgaccaacg ggcttttctt
      181 cctggatgtg ctaggcgacc cactggtgca gtctcagtgg gatgaactct cgcaattgat
      241 caccagtatt ttctttgacg gcgtggatcc gcgcccatag ggcaacctga taaccgaaaa
      301 ggatcgttat gtttttgaag aaccttttcc gccgaaaaat acgcaccgca cttaccgtgc
      361 tgggtatcag catcggtgtg gctgctattc tcggtttggg tgccatggca agtgggctgg
      421 aagctggtta tggctccatg ctctctggca gcaaggcgga cttgatcctc agccagccgg
      481 actcgtttga tatgagttac agcgtggtgg gtgaaaatat tggcgtgcag cttgctgcaa
      541 tgccggaaat ccatgccttg agcagcatgc tggagggatt tgtccaggca gagaacgagc
      601 catacttctt tgtttttggg tatccagagg acagtttcat cctggaccgc ttccagatcg
      661 tcgatggagc ggcgttatcg tcccgagaag cccggagtgc ccatggcaag ccgatcctgc
      721 ttggttcggc ggcggctgag gtgctggata aagcagttgg cgacagcatg cgcctgaccg
      781 gtagcgttta tcggattatc ggcatttacc agacaggtga tgcctttgaa gactctggtg
      841 ctattttgcg catgcaggac gcccaggagc tattggggaa gcctcgccag gtcagtgtgt
      901 tctatataca gctgaaggat cccgccttgc gggagcgctt tatcaagcgc gtcgagcgcc
      961 agatgcctga gctggagttg agcggggtgc aggagttcca agataagcag atcatgtcgg
     1021 acatgtttgc gggttatgtg tgggtgatcg gcggtctggc gatcctgatt ggcggagtgg
     1081 gcatgatgaa tgcccagttg atgtcggtgt tcgagcgcac gcgtgagatc ggcgtgctgc
     1141 gctctgtagg ctggagccgc agccgcgtgc tgtggatgat cctggctgaa tcgatgtttg
     1201 tctgcctggc aggcggagtg ttgggactgg gcctgggtta tttgctgttg gacgtcatct
     1261 caagaaacac agttattttt ggagcaagta cggcatccat cggcgctagc tcgttgcagc
     1321 aggctttcat cgtcgtggtt gtgatgggat tggtagctgg tctctatcca gcctggcggg
     1381 catcccgctt gcagcctgtg gaagcgctgc gctatgaagg cggtgctgcc agtaaagcac
     1441 gccgtttgcc cgtgggcggg atggcggtgc aaagcttgcg gcaacgggta acccgaacat
     1501 tgctgactgt catggtgatt ggcatcacgg tgggcgccat cgctgcgctt gagggcttgc
     1561 tcctcagcat gaaggcagat atgaccgcca tgttttccag cggcggcaac cagatcatga
     1621 tccgccaagc agatgtcagc gatacgtctc ttagtgccat cgatgagcgc attggcgata
     1681 aaatcgcggc gctgtctgaa gtcgagaccg tgagcggagt tatgatcgca gcggtcgtat
     1741 tgcccgacac gggtggtttc ttcatcatgc tgggctatgg acctaatgag caggcaattc
     1801 gccgtttccg catcgtagat ggggagccac taaaaagcaa ccaccaaatc atcctcggca
     1861 agtctatggc cgatgccatg aaaaaaaacg tcggtgatac cattgattta agcggcgcac
     1921 gctttcgcgt tgtggggata tatgagagca acatctcctg ggaagaatta ggcggcgtcg
     1981 cttccttgcg ggatacccag ttattcattg gtcggcctcg caaagttacc atgtttgctg
     2041 tttcagtacg cgacccgtct caggcggagg aagtggttga gaagatcaat gcaacattcc
     2101 ctgaagtgca cgcctcgctg agtggcgaat ttgccgatca gatgcccgat tttgaagctt
     2161 cggaaggtat gatgagcggc atctcggtca tggcgatctt catcggtggg gtgggtgtgc
     2221 tgaataccat gctgatggca gtctttgagc gcacgcgcga gattggcgta ttgcgttcgc
     2281 taggttgggg acgcagcgcc atcttgagta tgatcctgcg cgaatcgctc ttgttgggct
     2341 tgctgggcgg aggggctggg atagccatcg ctttttgcct ggtccaattg gcttcaatcg
     2401 caccaatagt gggtgattac ttcggtgtca cttggcagtg gcaaatcttc gcccgcgcca
     2461 tcgggattgc tatcatgctt ggtttactgg gcgggcttta ccccgcctat cgcgccacac
     2521 gattgcagcc tgttgaggcg cttagatacg agtgatttgg agagttcaaa tgcaaaagaa
     2581 atcgctttct ctcttgttta tatctgtact caccggactt gttctggttt cggggtgtga
     2641 tgccttgggg cagcgcgcta cgcctacacc ggaagcggtt gttgaggagg acttcgttcc
     2701 actggttaac gctactgggg tagtcgtacc ggcgcgattc actaccttga gtatgtctgc
     2761 cagcggagtg gtagccgagg ttttggtgag cgaaaatgat attgtctcgg ctggggagcc
     2821 attggtgcgc cttgaaggga aagaggattt ggaggctgcc atagctgccg ctcgtttcga
     2881 agtggcagct gcccagcacg accttgactt actgtataaa gacctggatt tgcgtcttgc
     2941 caacgcccag caggtcgtcg tagatgccaa acaagcattg cgggatgcag aacgccgcca
     3001 gtataacgtc gaacgtgccg ccaagcaggt agatatcgac caggcgcgcg ccaatctggt
     3061 gcttgctcgc gacaagctgg agaaggcagt ggaagatttc gagccatacg agaataaacc
     3121 tgaggataat ctgacccggg ccgcgttgtt gagcgcaaaa gcgtccgcgg aggagcaata
     3181 tgattcgtct gtgcgcctgc tgaataacct gctggctggc gcgaatgatc tggatgtcgc
     3241 cgaggcgaat gcagaagcgg aagttgcaaa ggcgcaattg gctgttgcgg agcgcgaggt
     3301 tgagttgctc aaggtaggcc cagatccgga ggatgtcaag ctcgccgaag ggcgactggc
     3361 gaacgctaaa gtccagctca ccagtgctga agcggctctt ggcgatctgg aactgctggc
     3421 tccatttaat ggtacggtca ccgagctata tatcaagcaa agtgaatgga tctcattcgg
     3481 tcagtctgtg ttgttattgg cggacctcga ccacttgcgc gtggagacca ccgacctaaa
     3541 tgaaattgat gtcgcccgta ttgatgaggg cagcacaacc ctgattacat ttgatgctct
     3601 gccggatgtg tctacccaag ggcaggtcat ccgtattgca cccaaggcct ccgcagggtc
     3661 tggggttaac tatactgtgg tggttgagat ggatgagatc ccgaaaagtt tacgctgggg
     3721 catgacggcg tttgtggata tcgaagttca ggaatagagg atttatcaat ggcacctgaa
     3781 tggattgtag aaactcgtga tttgacgcga atatatggtg atggcgaaga gattcacgcg
     3841 ctggatggcg tgaatttgca gatagcgccc ggcgaggttg tagcagtgat gggaccaagc
     3901 ggcagcggca aaagcacctt gctgaacatg attggtgcgc tggataaacc gactcggggg
     3961 caggtgttcg tcaacgggca agacctggct aagatccgca ataaggatgc tttccgcgct
     4021 aagacggttg gcttcatgtt ccagttacat aacttattgc cgacgatgac cgcacgcgag
     4081 aatgtggaag tgcccatgat ggggcatcgc ggcctgcgta cccgccataa gcgcgccaaa
     4141 gagctcttgg agctggtagg tcttccagac cggatgaacc acctgcccaa ccagctctcc
     4201 ggtgggcaga ggcagcgcgt ggcggtggcg cgtgccctgg caaatgaccc tcccctggtg
     4261 ctcgcggatg agcccactgg cagcctggat actgtggcag gcatggatct gctcaaatta
     4321 ttacacgagc tcaatcacag ccaggggacc acattcctgg tcgtgacaca tgacatcgct
     4381 gtggcgcgcc agacgcaccg cgtggtggtc atggcggatg gcaagatcgt gcgcgaggac
     4441 ctgattggct cccccctgga ggaagacctg aagatgtggc gtcactcggg gctgggcagg
     4501 cgaattctcg aagaagagat ggatgtgata cagagattgg ggatcgacga aagccaggct
     4561 cgggttctgc gggaaatttt cacttccgct aacagtgact aggtgatatc tacccgtgta
     4621 cccatttggc gggtgaacca gttttttgcg ggtgcaacgg gtgttgtcca gcgataaagc
     4681 cacaaagcgt cttgcgtgcg catattgaca cagccgtggc tcatcatgcg cccaaaatta
     4741 tcgtgccagt aggtcccgtg gaaggcgtac ccgtctttgt gaaagaaaca cacccagggc
     4801 acacctggta gctcgtaggc ttcaatgtca tcggtcagat tgccatcccc catgtggcgg
     4861 gatggcattt tggtttgtac ataaaaccta ccgcgcggtg tttcggtagg cagcatgccg
     4921 gggggcggct cgctgggtat gcccgtggag actttactgg tgaaaacgat ctggtcgttt
     4981 tcataggcgg tcagggtttg gtctgcgatg gagacggcaa tgcgcttgtc atcagggggc
     5041 acccgaggtg agattgggct cagttcatcg ctggcaacag gccgcacgtg aaaagacggg
     5101 acataatggt gaacatgaag caggtcatct gtcagtcgat accaggttct gccatctgga
     5161 ccgctatcca ggccggttat ccaatggatc gattggtaat acaggcggta aagcggtgcc
     5221 cactggttgc cgggcatttt tcgataggat tgtgtaaagg gcacactaat ctcgcccagt
     5281 tggccggttt cggggacggc ttccaaaacg tcgcggtgaa aatgagcccc atccaggcgc
     5341 tgcaggcgac cgctgtgggc ataaccttta gagaggcggt accagcgcgg gttgtagttg
     5401 ggaccatcgg gggagagggt ctcttcgtgg atatcgacaa tctgatcgcg cttgaggttg
     5461 ccgatacgtt cgctttgaaa ggaggcttgg ctatagcggt agaccagtcc cgtagccacg
     5521 cgcgccttgc cagcaagacg gggtggttca tcatctggaa gcaaaagata ggggctggat
     5581 gggtgggtga agagcgaggc tgccgctgcg cccccaagct ttaggaagct tcgccgggtg
     5641 atcatccgat ctctaccgta gtaccattac cgcggcgctc ccagtcggtg tgcgattcga
     5701 taggtgtttt gaaaacgggc gtggaccagc ggaagagcca caaggcatct tcgttgcgca
     5761 ggttgacaca tccgtggctc atctgaatac cgaaattgtt atgccagtag gtaccgtgga
     5821 aggcgtagcc cccgggaaag gcgaaaaagc acgtccaagg cacgccaggc agcgagaagc
     5881 cgccatttct gagcgcatcc ggatttccgg tgatgaaacc catgtgcttg ttgggcattt
     5941 tcgagtaaat gcggaatctt ccggtggggg ttgcggtcgg cagatcttcc ggggagcggc
     6001 gggcgctggg gatgcccgtc gagacttttg tcgagagcat cactttatcg ccttcatagg
     6061 cggtcagggt ctgccgccca attgagacct cgatgcgctt ctgttctggg ggcacgtcag
     6121 gggagatggg ggaaatctcc tcatcggaga tggggcgtag atgtgcggtg ggcacgaaat
     6181 atttcaaatg ggcgtcgatc tcggatgtca attcatacca ggcctcacca tctggcccgg
     6241 cttcgatgcc tgtgatccag tgcgtagtcg tatagtagag gggcgtgcca tcccagggtt
     6301 gccagccttc ccaggacgaa tactggtagg cgatggtgta cggtacggtc acctcgcaca
     6361 gctgacccgc ttcggggata gcgctcaagg gctcgttatg gcgcacttta acccgctgca
     6421 ggtaggcgct gtgcaggtag cctccccaca cccggtacca cagcgggttg tatgctggcc
     6481 catctggcga gatgacttct tcgtagatat gcacgatctg gtcgcgcagg cggttgccaa
     6541 cgatatcgct cttgtcgttg ggttgagagc gcacgtcgac ttgctggatg gtgatacggg
     6601 caatgtcgcc gtagtcttgc tcgtcaaggc gtggaaaaac aggccccagg gcgataccgg
     6661 cgaccgccat gccccccatc ttgaggaagc ctcggcgtga aaggcgattt tgcatagtcg
     6721 cgtgattata acactatccc acactgtcct tttccctcca atagggtgga attactctaa
     6781 taagatggac ttatttctgt cccagcgggt gtggaaaaca ggatttgtaa aaaattttac
     6841 gagagatttg ttgcaattat gtggaaaata tgatattatc aatttgcgag cgtccgggtg
     6901 cccccctcac ccgggcgctc gcaccttaac ataaagtggg ttttctggca aacccatgac
     6961 agttatagaa ttatggcgcc tctttagaaa gggcgtagac ccaggaatag gcctcctgcc
     7021 agtacatgta ggcagggtta tagtaaaccc ccttgatcca gagctggacc gggatcatat
     7081 aggtg
//
LOCUS       DBNGSX010000498         3014 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1020483, whole genome shotgun sequence.
ACCESSION   DBNGSX010000498 DBNGSX010000000
VERSION     DBNGSX010000498.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1020483"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..110)
                     /locus_tag="AC16CB_14770"
     CDS             complement(<1..110)
                     /locus_tag="AC16CB_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR3343783.1"
                     /translation="MPTLNRVQLIGRLGRDPEVRHTPSGKKVTSFSLAVDR"
     gene            277..942
                     /locus_tag="AC16CB_14775"
     CDS             277..942
                     /locus_tag="AC16CB_14775"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HIR3343784.1"
                     /translation="MYIAIEGVIGVGKTTLARLLQPVFQAELLLEVFEENPFLSDFYA
                     DRERYAFQTQIFFLLSRYHQQRNAVPEILKRDMNLLADYTFEKDALFARINIKGDELE
                     MYHRVHEALAEKIPLPDLIVYLRADTDVLMQRIALRDRPYERNMERDYIDQLNRAYED
                     FFVDARASEGHRRSPVLAVDTNQIDFVRRPADLKSMEQRIRQALRLSPFQEELPIKMS
                     SSG"
     gene            988..1992
                     /locus_tag="AC16CB_14780"
     CDS             988..1992
                     /locus_tag="AC16CB_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343785.1"
                     /translation="MRINRDILLKIAEETVSKVVRRDHSVLAAYLCGALLEEDFLLGG
                     TADIDLAFIHLDTPPVEREVQRLTDEIHLDIAHYDQGDFQQARNLRMHPWLGPTIYAC
                     KVMYDPQHFMDFTQASVRGQFYRSDYILGRATQQVEHARQIWLGFASQVKNKPGPRDL
                     ITYLRAIEHAANAVASLDGPPLPERRLLIGFPARAEATGRPGLYPALLGMLGAPQVDG
                     DELKSWLPSWQGALESLPKGAIPARLNPCRHVYYRRSFEEILDGERPADILWPLLRTW
                     TIAAASLSDQEEGYTAWRRACQRLGLLGEGFSERVMALDAFLDLVEETIEDRSRQYGE
                     "
     gene            complement(2025..>3014)
                     /locus_tag="AC16CB_14785"
     CDS             complement(2025..>3014)
                     /locus_tag="AC16CB_14785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3343786.1"
                     /translation="ISFEMIRYLTERLPVMVCPRWVYTRTQPIAIQDMLDYLIAALET
                     PESIGKIIEIGGSDVVTYAEMMTGYARARGLKRWLVPVPVLTPRLSSYWVHLVTPIPA
                     AIAQPLIAGLHNEVVVRDHSARCIFPTIQPITYQEAVQAALDSLEAAQVETTWNDALT
                     TSQNDPTPVLLTTQDGMILEQRQLLLSLPSEAIFRAFVGLGGKHGWLYWNWAWEIRGM
                     VDRLIGGVGLRRGRRDPEGVRVGDAVDFWRVEAVEPNQSLRLRAEMKLPGRAWLQFQV
                     RPHSNGQTLLQQTAFFAPKGLFGLLYWYALYSIHQLMFSGLIQRIAERAKLFT"
BASE COUNT          651 a          802 c          883 g          678 t
ORIGIN      
        1 cgatcgaccg ccaggctgaa gctggtgacc ttcttgcccg aaggtgtatg tcgcacctcc
       61 gggtctctgc ccaaacgtcc gatcaactgt acgcggttta gagtaggcat gtctttcttc
      121 cttagacggt gtctttctcc agtttagcgc gggtttgcgt aaagtcaaga aaatggtgtg
      181 agttaagaat tgcggggaaa ttgttcgaat ctgttgcgca ctccgccaat cgctggtatc
      241 cttaccacaa ccatcatctc acaagggaat cataatatgt acatcgcaat cgaaggcgtc
      301 atcggcgttg gcaagaccac cctggcccgc ttattgcagc cggtattcca ggctgagctg
      361 ctgctggagg tctttgaaga gaacccgttc ctctcagatt tctacgctga ccgtgagcgg
      421 tatgcctttc agacccagat tttcttcctg ctcagccgct accaccagca gcgcaatgct
      481 gtccccgaga tactcaagcg ggacatgaac ctgctggcag attatacgtt tgagaaagac
      541 gcactgtttg cccgtatcaa tatcaaagga gacgagctgg aaatgtacca tcgggttcat
      601 gaagcgctgg ctgagaaaat cccactgcca gatttgattg tttatctgcg cgctgatacc
      661 gatgtgctca tgcaacggat tgctctgcgc gaccggcctt atgagcggaa tatggagcgt
      721 gactacatcg accagcttaa ccgtgcttat gaggattttt ttgtcgatgc gcgtgccagc
      781 gaggggcacc ggcgatcccc tgtgctggcg gtcgatacga atcagatcga ctttgtaagg
      841 cggccggcag atctgaaatc gatggagcag cgtatccgcc aggcgctacg tctatcacca
      901 ttccaggaag agcttccgat aaagatgtca tcgagtggat gatgcgagat aggggagcgc
      961 agtggagcga gtcaggagaa ttaatctatg cgcattaatc gcgacatcct gctgaagatt
     1021 gccgaagaga cggtttccaa ggttgtccgc cgagatcata gcgtcctggc tgcttacttg
     1081 tgcggggctt tgctggaaga agattttctg ctgggcggca cggcagatat cgatctggct
     1141 tttattcatt tagatacgcc ccctgtcgag cgcgaagtgc agcgcctgac cgatgaaatc
     1201 catctggata ttgcccacta cgatcagggt gattttcaac aggcgcgtaa tttgcgtatg
     1261 cacccctggc ttgggccgac aatctacgcc tgtaaggtga tgtatgaccc ccaacacttc
     1321 atggatttca cccaggccag tgtgcgcgga cagttttacc gctcggatta catcttgggt
     1381 cgcgctaccc agcaggtgga gcatgcccgc cagatctggt tgggtttcgc cagccaggtc
     1441 aaaaataagc ctgggccacg cgatttgatc acctatctgc gagccataga gcacgctgcg
     1501 aacgcggtcg ccagcctgga cggtcccccg ctcccagaac ggcgattgct gattggtttt
     1561 cctgcgcgcg ccgaggcaac cgggcgacct ggcctgtacc ctgccttgct ggggatgttg
     1621 ggtgcgccgc aggtggatgg cgatgagctt aaatcttggc tacccagttg gcagggcgcc
     1681 ctggagtcgc tgcccaaggg ggcgatccct gccaggttga atccctgccg ccatgtttac
     1741 taccggcgtt cgtttgaaga gatattggat ggggaaaggc cagccgatat actctggccc
     1801 ttattacgca cgtggacgat cgctgccgca tcgctctcgg atcaggaaga agggtacact
     1861 gcctggcgaa gagcctgcca gcgcctgggt ttgttgggag aggggttcag cgagcgggta
     1921 atggcgctgg acgccttttt agatctggtt gaagagacga tagaggatcg ctcgcgccaa
     1981 tatggggagt aggttggcgt tccaatgttt cacgtgaaac atccttaggt gaagagcttg
     2041 gctcgctcgg caattctctg gattagaccg ctgaacatta attggtggat cgagtacaga
     2101 gcgtaccaat agagcagacc aaataagcct ttcggcgcaa aaaatgctgt ttgctgtagc
     2161 aaggtttgac cgttggaatg aggtctgacc tggaattgca gccaggcacg cccaggcagc
     2221 ttcatctcgg cgcgcaggcg cagggattgg ttaggctcaa ccgcctccac ccgccagaaa
     2281 tcgacagcat cccccacgcg cacgccctcc ggatcacggc gaccgcggcg caagccgaca
     2341 ccgccgatca gacggtcgac catgccgcgt atttcccagg cccagttcca gtatagccag
     2401 ccgtgcttgc cgcccaggcc gacgaaagcc cgaaaaatag cctctgaagg aagcgacaga
     2461 agcagctggc gctgctctaa aatcatcccg tcttgggtgg taaggaggac cggggttggg
     2521 tcgttctggc tggtagtcag ggcatcgttc caggttgtct caacttgggc ggcctccagg
     2581 ctatcgagtg cagcttggac ggcttcctgg taagtgatgg gttgaatagt cgggaagatg
     2641 cacctagcag agtggtctcg cacgacgacc tcgttgtgca gcccggcaat caacggttgt
     2701 gcgattgcag ctggtattgg ggtgaccagg tgcacccaat aggaagagag gcgtggggtc
     2761 agtactggta caggcaccag ccagcgcttc aaaccgcgcg ccctggcata gccggtcatc
     2821 atctctgcgt aggtcacgac atctgaccca ccgatctcaa tgatctttcc gatgctctcc
     2881 ggggtttcca gagccgcaat aaggtaatcc agcatgtctt ggatagcaat cggctgggtg
     2941 cgtgtgtaga cccagcgcgg gcagaccatg acaggtaagc gctcggtcag gtagcggatc
     3001 atttcaaatg aaat
//
LOCUS       DBNGSX010000499         6383 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_743148, whole genome shotgun sequence.
ACCESSION   DBNGSX010000499 DBNGSX010000000
VERSION     DBNGSX010000499.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6383)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6383)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6383
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_743148"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="AC16CB_14790"
     CDS             complement(<1..416)
                     /locus_tag="AC16CB_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343787.1"
                     /translation="MSQETTSTNPDRGTEILDLTWKGLYQIGGAAALICALMYLVTLG
                     VYVPANLVSPPPETVLEWFLVFEDSPITGLFFLGLADIIIMILWGPMSLALYIVLKEA
                     NRIWSLIAIPFVFVGMAVYLATNTAFSMLSLSQEYAV"
     gene            complement(622..1467)
                     /locus_tag="AC16CB_14795"
     CDS             complement(622..1467)
                     /locus_tag="AC16CB_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HIR3343788.1"
                     /translation="MKIYAIQTGTVVIRPNQVQGKGAGFVRLVNTFSDRRWTEPLPIY
                     VWVIEHPEGIIVIDTGETAKAALPGYFPSWHPYFKNVIIQVKQGQEVGEQLQTIGIHP
                     NDVRWVILTHLHTDHAGGLHHFPKSEILVVRRAFELATGFAGQVRGFLPHRWPSWFAP
                     KLIDLAPNPFSVFPESFKLTSAGDVIIVPTSGHTEAHISVVLQDADFSYFFAGDASYS
                     EQLMLEQQIDGVAINYREASQTLERIRQFAYSTPCIYLPTHDPESGNRLATKRLVPLD
                     GSHPS"
     gene            complement(1614..2147)
                     /locus_tag="AC16CB_14800"
     CDS             complement(1614..2147)
                     /locus_tag="AC16CB_14800"
                     /inference="COORDINATES: protein motif:HMM:NF015438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR3343789.1"
                     /translation="MHNSFNNGSVVAVCKKAEPGLPKDVVDVIQLLENFGVEGDYHAG
                     KLVRHRFLAKKYPNKLNLRQVLIIDKTIHAEIEAQGIHIRAGMMGENILVDGISIMAL
                     PVGTQVGIGEAILELTEVRNPCYQLNEMHPHLLKAVAKKVDGKVLRNAGMMARILKGG
                     MVQIGDPVIVYNKPAEL"
     gene            complement(2566..4920)
                     /locus_tag="AC16CB_14805"
     CDS             complement(2566..4920)
                     /locus_tag="AC16CB_14805"
                     /inference="COORDINATES: protein motif:HMM:NF046195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HIR3343790.1"
                     /translation="MKQKHLIFGILILGLVLVLSACAGPAGPAGPQGPEGPAGPAGAA
                     GEPGAPAEVSVAEPELVVQPETCAICHKGAGDKHQASYDELYQDGVIQVTDLAYRFSA
                     PDKSVVTFKMTKNGAPFNANDADNLGIYFAPYTGTNFQFEPAAGRQSLKGDITCDSAG
                     ACTSTLVGDAPNVGSTPGLVVLYGRDEDVGRLPARIAQTKYPFAAILETGSGVDYVSA
                     ANNAGCEKCHTDPYLKHGYIYAQLNQDPATDFYTCKACHLDNGEGGHLEWQLLVDNPE
                     LAAGYFAGDVELTDEQKAQYAYNTSLMNDVHMSHAMEFPYPQSMSNCVTCHEGKLDLV
                     LSDANFNITTCKSCHPVTGTVGPVKEGQEEPSYDTTTLALKTILPPAIHGSLDLDTAD
                     CLSCHGEGKAAPPFNQIHTGYNDVIYTADGLKYSEAISVTVDGASFDGSMLNIQFGAV
                     ETQDLEGLDVADIAPTVMVGLYGWDTKDFIIGPHERLFDDNGDGTIDRSDGRALEYGV
                     GEENPRFTTVSAGSGKWEVTADLSAWADMIADGTVMRVEIGVMPTLENADGVVLALNA
                     PSRTFDLGANAFVDDFYGPLVKVVDGCNNCHEALATTFHSPDRGGNVVVCRMCHITKS
                     GGSHLEMQSRSIDSYVHAIHSFQAFDIGDIDFADPVEAMHYEHHIEFPYPKHGITDCS
                     SCHVDGTNNVPDQSKSLPGLLSASDPVNGWDRKIGEVPEYVTGPASRACGGCHRAVMI
                     KEDEAVELTAFNLHTKQGGYLIEGGEDSSGTLMSVINEIMALFK"
     gene            5045..5200
                     /locus_tag="AC16CB_14810"
     CDS             5045..5200
                     /locus_tag="AC16CB_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343791.1"
                     /translation="MEVGVKPFPYGYFSVNSVAVRQSEADLVVERHTPDPRFGLMTYH
                     NERLDGR"
     gene            complement(5967..6039)
                     /locus_tag="AC16CB_14815"
     tRNA            complement(5967..6039)
                     /locus_tag="AC16CB_14815"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6003..6005),aa:Glu,seq:ctc)
BASE COUNT         1604 a         1630 c         1481 g         1668 t
ORIGIN      
        1 acagcatact cctgactaag cgaaagcatg gaaaaggctg tattggtcgc aaggtaaacc
       61 gccatcccca caaagacaaa tggtatggcg atcaacgacc agattctgtt agcctctttg
      121 agcacaatgt atagagcaag agacatcgga ccccagagaa tcatgatgat gatgtcggcc
      181 aacccgagga aaaagagccc agtgatcgga ctatcttcaa agaccaggaa ccattcgaga
      241 acggtttcgg gaggtgggct gaccaggttg gcagggacgt agactcccaa ggtaacaaga
      301 tacatcaagg cgcaaatcag ggctgccgct ccgcctattt ggtaaagccc tttccatgtg
      361 aggtccaaga tttccgttcc acgatctgga ttagtgcttg ttgtctcctg tgacatggct
      421 tgctccttaa tcttggcgaa ttttgaagat aatcctttcg atggaaaaaa tgtaaaactc
      481 aaagcgattc agtgcgacag cgcctgacag gttgcgttac ctatcactga ttttaacgga
      541 tgggtcatcg gcatacatct cttctagcaa gggaatgacc acaagtcaaa ctgtgccgat
      601 ggcagtcagg ggctcattgt gttagcttgg atgcgaacca tcaagcggca caagcctttt
      661 agtcgcaaga cgattgcctg actctgggtc atgtgttggc aaatagatgc aaggtgttga
      721 atatgcaaat tgccgaattc gttcgagggt ttggcttgct tcgcggtaat taatggcaac
      781 tccgtcaatc tgttgctcca acatcaattg ctcggagtag gaggcgtcgc ctgcaaagaa
      841 ataagagaaa tcagcatctt gtaatacaac tgagatatgc gcctcagtgt gaccggaagt
      901 tggaactatg atcacatccc cagcacttgt tagcttaaaa ctttcgggga atacactaaa
      961 aggattggga gcaaggtcta ttaatttggg ggcaaaccat gacggccagc ggtgtggcaa
     1021 aaagccgcgc acttgacccg caaagccagt ggctaattca aaggctcgtc tgacaacaag
     1081 tatttctgac ttgggaaaat gatgtaaccc accagcgtgg tcagtgtgga ggtgagtaag
     1141 gatgacccag cgaacatcat tgggatgaat acctatggtc tgtaattgtt cgccgacttc
     1201 ttgtccttgt ttcacttgaa tgattacgtt cttgaagtaa ggatgccaag atgggaaata
     1261 acctggcaaa gcggctttgg cagtttcgcc tgtatctatg acaataatcc cttcaggatg
     1321 ttctataacc cagacgtaaa taggaagcgg ctctgtccaa cgacggtcag agaatgtatt
     1381 cactagcctg acaaagcccg ccccttttcc ctggacttga tttggtctaa ttacgacagt
     1441 gcctgtttgg attgcataaa tcttcatgta ttttcctttt gaaacaaaga accaaacctc
     1501 gtaaatcgca ccccctactc tcggtcgcag cccagccttg tactgggggg gtagccggcc
     1561 caatatttat cgaacaagaa gtcccccaaa tattatcgcg gaacaagcag atgtcacagt
     1621 tcagcgggtt tgttatacac aattactggg tcgcctatct gtaccatgcc tccttttaga
     1681 atgcgcgcca tcatccctgc attacgaagc acctttccat cgactttttt cgctactgct
     1741 ttgagcagat gtgggtgcat ctcattgagt tgataacatg gattccgaac ctcagtcaat
     1801 tccaaaatcg cctcaccgat ccctacctgc gttcctaccg gcagagccat aatagagatg
     1861 ccatctacta ggatgttctc acccatcatc cccgctctaa tatggatgcc ttgtgcttct
     1921 atttcagcat ggatcgtctt atcaatgatc agtacctggc gtagattgag tttgtttgga
     1981 tattttttcg ccaggaagcg atgtcgtacc aacttcccag catggtaatc accttcgaca
     2041 ccaaaatttt ctaaaagctg tatgacatcg acgacatctt ttggcaatcc aggctcagct
     2101 tttttgcaaa cagcaactac cgacccgtta ttgaatgaat tatgcattag gttgtcctcc
     2161 acacctggat aacaactttc gattcctttt acctgtcata atattaatgc agagcgcgtc
     2221 ctttggcttg gatatttgat gaaaccggtc gtgtttgccc gagcctcgtg taattataac
     2281 caatgaacct tgtcgagagg taattgttca atagctaacg ccatgtcaaa tcagcctgtg
     2341 atattttgcg cggtcttagc ttggacctgt tctttgaaac tcgcgattct tttcagccag
     2401 gacactgaat ctaccgatgt taagaaattc caagaaaatg gatcaccttc acggtcaaat
     2461 ccactatacc agtgttgcct ccaccgttgt ctttaatcaa ttaagggccc cgaagggccc
     2521 ttgttattga ctgaggtgag ccgaagccca cctcacattc ttcaattact tgaagagtgc
     2581 catgatctca ttgattacgc tcatgagagt gccgctggag tcctctccgc cttcgatcag
     2641 ataaccaccc tgcttggtgt gcagattaaa ggccgtaagc tcaacagctt cgtcttcttt
     2701 aatcattacc gcacggtgac atccaccaca ggctctcgaa gctgggccgg tgacatattc
     2761 tggcacttca ccgatttttc tatcccagcc attgactgga tcggatgccg aaagcagacc
     2821 tggcagcgat ttggattgat ccggcacgtt gttcgtgcca tccacgtggc aggagctgca
     2881 gtctgttatt ccatgcttag gatacgggaa ctcgatgtga tgctcatagt gcatggcttc
     2941 caccggatca gcaaagtcga tatcaccaat atcgaatgct tggaatgagt ggatcgcatg
     3001 gacataggaa tcgatcgacc tcgattgcat ttccagatga gaaccgccac tcttggtgat
     3061 gtgacacatc ctgcagacaa ctacgtttcc acctctatcc ggtgaatgga aggttgttgc
     3121 aagagcttca tggcagttgt tacagccatc cacaacctta acgagagggc cgtagaagtc
     3181 atcaacaaat gcattggcgc caaggtcgaa ggttcgggaa ggagcattca gcgcgagcac
     3241 aacaccatca gcgttctcca aggtcggcat gacaccaatt tccacccgca ttactgtgcc
     3301 atcggcgatc atgtcagccc acgccgacag gtctgcagtg acttcccact tgccactgcc
     3361 tgcagagact gtcgtaaagc gcggattttc ttcgcctacg ccatattcca gggctctccc
     3421 atcgctcctg tcgatggtgc catcgccatt atcgtcgaaa agcctctcgt gcgggccgat
     3481 gatgaagtcc ttggtgtccc agccatacaa accgaccatc acggtggggg caatgtccgc
     3541 cacatcgagc ccctcaagat cctgggtctc aaccgcgccg aattggatat tgagcatgct
     3601 gccgtcaaag gatgcaccat caacggtgac ggaaatggct tcagaatact tcagcccatc
     3661 ggcggtgtag atcacatcgt tataaccggt gtggatctga ttgaacggtg gtgctgcctt
     3721 gccttcacca tggcaactaa ggcagtcagc cgtatccaaa tccaacgacc catgaattgc
     3781 tgggggcaat atggtcttca gtgcgagcgt ggtggtatcg taagatggct cttcttgccc
     3841 ctctttcaca ggtcctactg tacctgtcac agggtggcag ctcttgcagg tcgtgatatt
     3901 aaagttcgca tccgacaaca ccaaatccag tttgccttcg tggcaggtta cgcagtttga
     3961 catggactga ggatagggga attccatggc atgcgacata tgcacgtcat tcatcagact
     4021 ggtattatat gcgtactggg ctttctgctc atccgtcaat tcaacatcgc ctgcgaaata
     4081 ccctgcagcc agctctggat tatctaccaa aagctgccat tctaaatggc cgccttcacc
     4141 gttatccagg tggcaggctt tacaggtgta gaagtcagta gcgggatcct gattcaactg
     4201 ggcataaatg tacccatgct tgaggtaagg atccgtgtgg catttttcgc agccggcgtt
     4261 attggcagcc gaaacgtagt ctacgccgct accggtctct aatattgccg caaacgggta
     4321 tttggtttga gcgatgcgag caggtagtct accaacgtcc tcatccctac cgtacaaaac
     4381 gacgaggcca ggcgtactgc ccacattagg ggcatcccca accagagtac tcgtgcatgc
     4441 acccgcgcta tcacaagtta tatccccctt cagggactgt cttccagccg ccggttcaaa
     4501 ctggaagttt gtaccggtat atggcgcaaa gtaaatacct agattatctg cgtcattggc
     4561 attgaaagga gccccattct tggtcatttt gaaagttacc acgctcttat cgggagcaga
     4621 aaacctatac gccaagtcgg taacttgaat gacaccgtcc tgatacaact cgtcatagga
     4681 cgcctgatgc ttgtcgccag ctcctttatg gcaaattgca caagtctcag gttggacaac
     4741 tagttctggt tctgcgacag agacttcagc gggcgctcca ggttcgcccg ctgctcctgc
     4801 cggaccagcc ggaccctccg gaccttgcgg accagcgggg cctgcggggc ctgcacaagc
     4861 tgatagcacc agtacaagcc cgagaatcaa aatcccaaaa atcaggtgtt tttgtttcat
     4921 ttgcattctc cattctcata ttattaattt catacgtaaa ggcaggttct ctccattatg
     4981 tattcttgat gtataaacta cctcctttca ctttttgaca gaaatccgac gttgctgatg
     5041 aattatggaa gtgggagtaa agccctttcc ctatggatat ttttcagtta attctgtagc
     5101 tgtccggcaa tccgaagcgg accttgtggt tgagcgccac acgcctgacc cgcgcttcgg
     5161 tttgatgacg tatcacaacg agcgtttaga cggtcgctaa attggatata aaccgaaaaa
     5221 tggctgtttc ggtaaatgaa cggcgatgat agctgttttg ttcgttccat tcccctcccg
     5281 tattcacaaa tccggcgtaa gatttcacgg cgcggatggt tccagatcca ttatctcacc
     5341 agaaaacgac atcgcagcag gattgaatct tgatcttgcg cacccctttg gcggcggatg
     5401 tcttgtgtat gcattctcta agtcataagc gccatcaaca aaaattgttc aatttcggtc
     5461 cgcgtttgtt gctgtaacca gctactgttt cagtagtatc cacacccagg tgcattgttt
     5521 gccataacaa tgctgtcgca ttttctactc accctcccca aataacttat aattcggtgc
     5581 tcccaatccg gaaaaatcga tcttttagaa atcttgtctg ccccttttat taatgcttga
     5641 ttagttagaa ttttcacatt ctatcatgat tctccgtaat tgggtgatca aatttgtcag
     5701 gtgaatgaca gctgattgtc agaatccaag accatgtaat caacctgagc gacttcaagt
     5761 ggcaggtcgg caacaactag acaggctctg cggcttgagc gtgggcagtg aaggcgttct
     5821 gcaactttaa ttcgggagcc aagccggtag tgaccagttt cccttttcgt tcatcatctg
     5881 gtcagcaccc gggctgtggt cgaatgccac cactccttta taaatataga caaaaaccgt
     5941 tcaagatcaa cttgaacggt ttcggatgcc cccaagggga ctcgaacccc tgttttggcc
     6001 ttgagagggc cacgtcctgg gccactagac gatgggggct gagagcgcag aaattctacc
     6061 atgccaattt accaacgtca acatcccacc tcttcctcca gggcaattgc atatgccctg
     6121 aggggcatgc gcactcccgc aaagagagtg tttggggtgg gaaatcgtcc taatgcgatt
     6181 gccctacctc ttcctacttg gcacagcttt cacaggatcg aaacctatag agaagatgtc
     6241 tataaataag cggtagagca tcctgattaa atccacaaac acgctcttgt gataaccctg
     6301 tccacttggc agtataatag agccggcatg ccaaacacga tagggcttcc aggtatcgca
     6361 tgacaaccct aactctcccg ttt
//
LOCUS       DBNGSX010000500         7061 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_812530, whole genome shotgun sequence.
ACCESSION   DBNGSX010000500 DBNGSX010000000
VERSION     DBNGSX010000500.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7061)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_812530"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="AC16CB_14820"
     CDS             <1..1130
                     /locus_tag="AC16CB_14820"
                     /inference="COORDINATES: protein motif:HMM:NF024585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HIR3343792.1"
                     /translation="VQHHPRIQVLTAAEVCDVSGYVGNFQVQVRQADQLVNVSVGTIL
                     VATGFEAFDAHRKPELGYGEYPQVMTTLDFDRLARTDLLVNGKQPERVVFIQCVGSRE
                     RSSGDQNGRNPYCSRVCCMTVARQARLVRERLPDSKVTVFYMDVRAYGKGFEEFYDEV
                     RQHGVIYRRGNPSEIVRRGARVVVRAEDTLLGEPVEVEADLVVLAIGMTPVSDSAHLA
                     SLLKLARSADGYFLEAHPKLRPVDTALAGVFLAGCCQGPKDISESITHARAAAAAAMI
                     PMMRGQMPVEAATAFVDEEMCSGCNQCAQSCSFSALQLHPVRGVMKVNPVLCQGCSAC
                     ATACPSGAINVHRFTTDQVLGQIEAFSIVEPELVMSNGGVR"
     gene            1127..2155
                     /locus_tag="AC16CB_14825"
     CDS             1127..2155
                     /locus_tag="AC16CB_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343793.1"
                     /translation="MKTSLLLGTNGDALGVVRQFLQELWSYADLDGMLVPIYHADGIN
                     VSPYFVEDPSHLMLADPLAPMVSASSAKMVPQITDFNPKARLGFVWHSCEARAWQWLI
                     EQKNIAPGNWLIIGVDCLSSFSSKDFGWRVERAGNVERLTRETMRFARMGGIAPYRYR
                     DACQMCPSPVVQDADLNIELLGLPVKSVLIITAKDREIVNKLRLRELTDGEAPPSLVS
                     RREATLGVLSGRRRQAAERMEQGLSEDMPAEVGSLLQYFANCAPCQECLEACPIYMGE
                     FSPATNGGAKAISAATRWLESCISCGMCDDACPKQMPLTAMFRHIHHALQDDINLTPA
                     SNLAGIAS"
     gene            2165..2614
                     /locus_tag="AC16CB_14830"
     CDS             2165..2614
                     /locus_tag="AC16CB_14830"
                     /inference="COORDINATES: protein motif:HMM:NF024634.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3343794.1"
                     /translation="MPLRQQTDPQRIGSILHHVFSLPYPPVARCRLLSSGFGYGYMLK
                     TTDDLSGSKACLGCGCCMDACPVLARDSSRRIRSEARTSMALETLVGDDCDRCGNCAL
                     ACPQVDPTIKHFLVQTHLAEGMAELLVKATSDEMFVLDVMLSDWSSP"
     gene            2611..3276
                     /locus_tag="AC16CB_14835"
     CDS             2611..3276
                     /locus_tag="AC16CB_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HIR3343795.1"
                     /translation="MTEIFIIGQRYEVPEGLTILRAFEWAGFRLTRGVGCRGGFCGAC
                     GTVYRLNSDYHLRYALACQTVVQPGMVLGQIPFFPAQRPAYRLEELKPEGETFLDLYP
                     EILRCFGCNTCTKSCPQDLDVMSYMSAALRGDLQALADLSFDCIMCGLCVARCPAELV
                     PPNVALLGRRLYGKYLAPTADHLHRRVQEMESGIYLDAISELKNLPLADLKKRYNTRE
                     IEA"
     gene            3306..4436
                     /locus_tag="AC16CB_14840"
     CDS             3306..4436
                     /locus_tag="AC16CB_14840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3343796.1"
                     /translation="MDFEFSSDTLMLRDMLRRFVQKEARPLEMKYFTAGVLAPEEKAR
                     LHRSIEQMGLWGLTAPEQFGGGGLDLVNYCVIQEELGQTFIPVEIGEVTPLLFSCSGE
                     QVSGYLEPALAGERRAILAAREPGSCRPDNWTTHASVVEGGPSSGFRLNGNKVIGVQP
                     DPADFFIVIANGHERRFTAFLIDGDHPGLEVSANGEFTLALRDCEVGKEAVLGEPGGA
                     FGLGVQEAPRAWIQTGARTVGIVQRLMEMAVEYARDWESLGAPLAIRPAIQRMIAEIS
                     VELESSRWLVYHAAWLADKGETDETRFQAAQVRLATAEMLKRAVDRVTMIYAGPGPST
                     QIEPQRLIRSVVPMDALELTLENARAAISTQVLGLQNLGGDS"
     gene            4433..5506
                     /locus_tag="AC16CB_14845"
     CDS             4433..5506
                     /locus_tag="AC16CB_14845"
                     /inference="COORDINATES: protein motif:HMM:NF016328.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3343797.1"
                     /translation="MNVYRVLEVRDGDVVSTLREFMAEWWDYYQPDVLLAPVESADRR
                     GFRPKVINNPAELAGINPFAPVMQENAALAASRLIHDHAGEQVAVMLRPCELRAFVEL
                     RKRQREMESQDNVTVIGVDCLGTFTQADYRRHIDLREPDDVTAEVLSNAAEGGLRPQR
                     FRTACQICDWPAPRGADMTIGTIGVASNKHLLLIANDEKTDSNLGLNSLTDHLANEYQ
                     ASRRETVVGAIADARTGLRKSMIEDMQGVCRFDDLGCILAWFANCSLCGECLKACPIY
                     DNEFADLVNQAGALKVDRPPLKDLVSLSRWLASCAGCGMCEEECAMNVPLFLLISALS
                     HRIREKMHYRSGDPAQRLPWVSG"
     gene            5555..>7061
                     /locus_tag="AC16CB_14850"
     CDS             5555..>7061
                     /locus_tag="AC16CB_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3343798.1"
                     /translation="MSYPAIIRESMQKVEASRARRTQETHPRFTPQEKEEILRKFHPD
                     YIAEAFRGLRLGPNKGDRAPRELVDVLEAPSLLSSSYDLDREPDMTCDLLVIGGGGAG
                     ASAALLAQENGAEVLVTTKLRFGDANTMMAQGGIQAADKDNDTPAMHYLDVLGGGAFS
                     NDPDLVEALVTDAPMVIAWLESLGVMFDKEPDGVLRTIHGGGTSRKRMHAARDYTGAE
                     IMRTLRDEVRSRDNGKAASIRVWEFRPAIELLLDDKGQAAGAVLKDLDTGRLLYVQAK
                     AVIIATGGSGRLHYQGFPTTNHYGATGDGLVLAYRLGAKLSFIDTMQYHPTGAAYPEQ
                     ILGQLVTEKVRGLGAQVCNIDGEQFVFPLETRDVEAAAFIRECVERRAGVVTPTGQPA
                     VWLDTPLIEMLRGPGTVACELPAMVRQFKRFDIDLPGEPILVYPTLHYQNGGIAIRAD
                     GSTAIAGLFVAGEAAGGIHGRNRLMGNSLLDICVFGRRAGVSAALWAQGVQP"
BASE COUNT         1465 a         1775 c         2152 g         1669 t
ORIGIN      
        1 gggtgcagca tcaccctcga attcaagtgc tgacagcggc agaggtttgt gatgtcagcg
       61 gttatgttgg taattttcag gtgcaggtcc gtcaggctga ccagttagtg aatgtatccg
      121 tggggactat tttggtcgcc acgggctttg aagccttcga cgcgcacaga aaacctgaat
      181 tgggatatgg ggaatatccc caggtgatga ccacgttgga ttttgatcgc ctggctcgaa
      241 cggatttact cgtcaatggg aaacagcctg aaagggttgt tttcatccag tgtgttggct
      301 cgcgcgagcg ctcttcggga gatcagaatg ggcgcaatcc gtattgctca cgggtttgtt
      361 gtatgacggt tgcccgccag gcccgcctgg tccgggagcg tctgcctgat tccaaagtga
      421 ccgtgtttta catggatgtg cgcgcctatg gaaagggttt tgaggagttc tatgatgaag
      481 ttcgccagca tggggtcatc taccggcgtg gaaatccatc agagattgtt cgaaggggcg
      541 cgcgtgtggt ggtgcgcgcc gaggatacct tgctcggcga accggtcgaa gtagaagctg
      601 acctcgtggt gttggcgatt ggcatgacgc cggtttcaga tagcgctcat ctcgccagct
      661 tgctcaagct ggctcgttct gcagatggct acttcctgga agcgcacccg aagctgcgtc
      721 ccgtggatac tgctttggcg ggcgtgtttc tggctgggtg ctgccagggt cccaaagata
      781 tctccgaatc gatcactcac gcccgggcgg cggctgccgc agcgatgatc cccatgatgc
      841 gcggccagat gcccgtggaa gcagcgactg cctttgtgga tgaagagatg tgttcaggtt
      901 gtaaccagtg cgcacaatct tgcagctttt cagcgctgca attgcatccc gttcggggcg
      961 taatgaaggt gaatccggtg ttgtgccagg ggtgtagcgc ttgcgccacg gcctgtccga
     1021 gcggagcgat caacgttcac cgctttacca ctgaccaggt tttggggcag attgaagcat
     1081 tctcgattgt agagcctgaa ttagtaatga gtaatggagg tgtaagatga aaacgagctt
     1141 gctattaggg accaatggtg atgcattggg ggtcgtgagg cagttcttgc aggagctctg
     1201 gtcgtatgcg gatttggatg gaatgttggt gcccatctat catgccgatg ggatcaatgt
     1261 atctccctac tttgtggaag acccatctca cttaatgcta gctgatcctc tcgcgccgat
     1321 ggtttctgca agttcggcca aaatggtacc gcaaatcacg gatttcaatc caaaagcgcg
     1381 gcttggcttt gtttggcact cttgcgaggc gcgtgcctgg caatggctga ttgagcagaa
     1441 aaatatcgcc cctgggaatt ggttgattat tggtgtggat tgcctgtcta gtttctcctc
     1501 aaaagacttt gggtggcgtg tggagagagc gggcaatgta gaacgcctca ctcgtgagac
     1561 aatgcgcttc gcacgcatgg gaggcatcgc gccctaccga taccgtgatg cctgccagat
     1621 gtgcccttca ccggttgtac aggacgctga tctgaacatt gagttattag gactgcctgt
     1681 aaaaagtgta ttgatcatta cagcgaaaga ccgagagatc gtcaacaagc tccgtcttcg
     1741 ggaactcacc gatggagagg cccccccatc gctggtatcg cggcgtgaag ccacgttggg
     1801 ggtgctgtcg ggacgcagga gacaagcggc ggagcgtatg gaacaaggcc tgtcagagga
     1861 tatgcccgcc gaggtgggca gcttgcttca gtacttcgct aattgcgctc cgtgtcagga
     1921 atgcctagaa gcctgcccga tctatatggg tgagttttca ccagccacaa atggtggtgc
     1981 taaggcgatc tctgccgcta cacgctggtt ggagtcttgt atctcctgcg gtatgtgtga
     2041 cgatgcttgc cccaagcaaa tgccactgac cgcaatgttc cgccatatcc atcacgcctt
     2101 gcaggatgat attaacctga ctccagcctc taacctggca ggaattgctt cctaggaggt
     2161 tgccatgccg cttcgccaac aaactgatcc acagcggatc ggatctatac tacatcatgt
     2221 tttctctttg ccgtacccac ctgtcgcgcg ttgccggttg ctgagctcag gctttgggta
     2281 tggctacatg ctgaagacga ccgatgacct gagcggttca aaagcttgcc taggttgtgg
     2341 ctgctgtatg gatgcctgtc ctgtgctggc tcgagactcc agccgtagaa tacgcagtga
     2401 agcgcgcacc tccatggcat tggagacctt agtgggcgac gactgcgatc gttgcgggaa
     2461 ctgtgcactg gcttgcccac aggttgatcc taccatcaag cattttctgg tgcaaacaca
     2521 tttagccgaa gggatggccg aactactggt taaggccaca tcagacgaga tgttcgtgct
     2581 ggatgtcatg ttaagtgatt ggagttcgcc atgacagaaa ttttcattat aggtcagcgt
     2641 tatgaagtcc ccgagggttt gactattctg agggctttcg agtgggctgg atttcgcctt
     2701 acgcgtggtg tcggctgccg ggggggattt tgcggggctt gcggtactgt ttaccgcttg
     2761 aacagcgact accacctgcg gtacgcctta gcctgccaaa cagtggtgca gcccggcatg
     2821 gttcttgggc agatcccatt tttccctgcc caacgcccgg cttataggct ggaagaactc
     2881 aagcccgagg gtgaaacttt cctcgatctt tacccggaaa tactgcgttg tttcggttgc
     2941 aatacctgta caaagtcttg cccgcaggac ctggatgtga tgagctacat gtccgcggcc
     3001 ttgcgcggag atctacaagc tctggctgat ctctccttcg actgcatcat gtgtggcctg
     3061 tgtgtagccc gctgtccggc tgagctggtg ccgccaaatg tagccctgtt gggtcgtaga
     3121 ctgtatggga agtacctggc cccaacagca gaccatctgc atcggcgcgt gcaagaaatg
     3181 gagtcgggga tatatctgga tgctatttct gagcttaaaa atttgccgtt agcagatctg
     3241 aagaaacggt ataacacccg ggaaattgaa gcatagaccg attggtttga ggagtgaggt
     3301 aaccaatgga ttttgaattt tcatccgata ccctcatgct ccgcgatatg ctgcgccgtt
     3361 ttgtgcagaa agaggcccgg ccgttggaga tgaaatactt tactgccggg gtcctggcac
     3421 ccgaagaaaa agcccgcctg caccgttcga tcgaacagat ggggctgtgg ggtctgactg
     3481 cccccgagca gtttgggggt ggcgggctag acttggtgaa ttactgcgtt atccaggagg
     3541 agcttggtca gacgtttatc ccggttgaga ttggcgaggt gacacccctg ctgttctctt
     3601 gtagcggtga acaagtctct ggctacctcg agcctgcact ggcgggtgag cggagggcca
     3661 ttctggccgc gcgtgagccc ggaagttgcc gccctgataa ctggacaacc catgcttcgg
     3721 ttgtagaagg cgggccttcc agtggtttcc ggcttaacgg caacaaggtc atcggagtgc
     3781 agcccgaccc ggccgatttc tttatcgtca tcgccaacgg tcacgagcgg cgtttcactg
     3841 ctttcctcat cgatggcgac caccctggac tcgaggtttc tgcgaatggt gagttcactc
     3901 ttgccctgcg agattgtgag gtaggcaaag aagccgttct gggcgaaccg ggaggggcat
     3961 ttggcctcgg ggtgcaagaa gcaccgcgcg cgtggattca aacgggtgcc cgcaccgtgg
     4021 gtatcgtgca acgcttgatg gagatggcag tggaatatgc cagagattgg gagtctctcg
     4081 gcgcgccgct ggcaattcgt ccggcgatac agcgcatgat agcagagatc agcgtggagc
     4141 tggagagctc tcgctggctg gtttaccatg ccgcctggct agctgacaag ggagagacgg
     4201 atgaaacgag gtttcaagcc gctcaggtcc gcttggcaac tgccgaaatg ctcaagcgcg
     4261 cagtggatcg ggtgaccatg atctatgcag gtccggggcc ttctacccaa attgagccgc
     4321 agcggcttat ccgcagtgtt gttcctatgg acgcgctgga actaacctta gaaaatgccc
     4381 gcgcagcgat ttcaacccag gtattaggcc ttcaaaactt aggaggtgac tcatgaacgt
     4441 atatcgcgtt cttgaggtac gtgacgggga tgtggttagc acactgagag agttcatggc
     4501 tgaatggtgg gattattacc aaccagatgt gctgctggcg ccggttgaat ctgctgatcg
     4561 gcgtggcttc cgcccgaagg tgatcaataa ccccgcggaa ctggctggaa taaatccctt
     4621 tgccccagtg atgcaggaga atgcagccct cgctgccagc cgtttgatcc atgaccatgc
     4681 tggtgagcag gtggcagtca tgttgcgccc gtgtgaactg cgcgcttttg tggagcttag
     4741 aaaacggcag agagaaatgg aaagccagga taacgtgacc gtcatcgggg ttgattgctt
     4801 gggcacgttc acccaggcgg attacagacg ccatatcgac ctgcgcgagc ctgatgatgt
     4861 caccgctgag gtgttgagca acgccgctga gggtggcttg cggcctcagc gctttcgcac
     4921 agcctgccag atctgcgatt ggccagctcc acgcggtgct gatatgacca tcggaacaat
     4981 cggtgtcgcc tccaacaaac atctcttgct tattgccaac gatgagaaga cggattcgaa
     5041 cctgggattg aatagcctga cagatcattt agctaacgaa taccaggcct ctcgccggga
     5101 gactgtggtt ggtgcgattg ctgatgcacg gaccggttta agaaaaagta tgatcgaaga
     5161 tatgcaaggt gtctgccggt ttgacgactt gggctgcata ctagcctggt ttgccaattg
     5221 ttcgttgtgc ggcgaatgcc taaaagcctg tccaatctat gataacgagt ttgccgattt
     5281 ggtgaatcaa gctggcgcgc tcaaggtgga tcgcccgcca ttgaaagatc tggtcagttt
     5341 aagccgctgg ctggcttcat gtgccggttg cggcatgtgt gaagaggagt gcgctatgaa
     5401 tgtgcccctc tttctgctga tctcagccct cagtcatcgc atacgcgaga agatgcatta
     5461 ccgaagcggt gatcctgctc agcggctccc ctgggtctcg gggtaaaacc cgtcgagttg
     5521 cgcaatctaa ttgtcctatt aatgggaggt ggaaatgtcc tatccggcca taattcgtga
     5581 atcgatgcag aaagttgaag ctagccgggc acgccgcacc caggaaactc atccgcgctt
     5641 tactccgcag gagaaggaag agatcttgcg taaattccat ccagactata tcgccgaggc
     5701 ttttcggggg ctgcgtctgg gtcccaataa aggcgatcgc gccccgcgag aattggtcga
     5761 tgtgctggaa gcaccttctc tcctgtcatc aagttatgat ctggaccggg agcctgacat
     5821 gacctgcgac ctgctggtca ttgggggtgg gggagctggg gccagcgcgg ctctgctggc
     5881 gcaggagaat ggtgctgagg ttctggtgac taccaagctg cgttttgggg atgccaacac
     5941 catgatggct caaggaggca tccaggcggc agataaagac aatgatacgc ccgccatgca
     6001 ctatctggac gtattgggag gtggggcttt tagcaatgac cccgatcttg tagaagccct
     6061 ggtgacagat gcacccatgg tcattgcctg gcttgaatcg ttgggggtca tgttcgataa
     6121 agagccagat ggcgtcctgc gcaccattca cggtggaggc accagccgca agcgcatgca
     6181 tgctgcccga gattacaccg gtgcagaaat catgcgcacc ttgcgggatg aagttcgctc
     6241 gcgagacaat ggcaaagctg cctcgattcg agtctgggag ttccgtccag ccattgagct
     6301 gctcttggac gacaagggtc aggctgcagg ggcagtgctg aaagacctgg atacagggag
     6361 acttctatat gtccaggcaa aagcggtcat cattgccact ggcggcagcg ggcgactaca
     6421 ttatcaggga ttccctacca ccaatcacta tggcgccacg ggcgatggtt tagtgctggc
     6481 atatcgcctg ggagccaagt tgtctttcat cgataccatg cagtaccatc ccaccggagc
     6541 agcctatcca gagcaaatcc tggggcaact ggtgacggaa aaggtgcgcg gcctcggtgc
     6601 gcaggtctgt aatatagacg gggagcagtt cgtctttcca ctcgaaacgc gagacgtgga
     6661 ggccgccgcc ttcatccgtg agtgtgttga gcgtagggct ggtgttgtaa cgcctaccgg
     6721 tcagcccgca gtttggctgg acactccctt aatcgagatg ctgcgaggcc ctggtacggt
     6781 agcatgcgag ttgccagcta tggtgaggca gttcaagcgc ttcgacatcg atctgccggg
     6841 tgagcccatc ctggtctatc ccaccttgca ctaccagaat gggggtattg ccattcgcgc
     6901 ggacggaagc acagctatag cgggtttgtt tgtggccggg gaggcagccg gaggtattca
     6961 tgggcgtaat cgcctgatgg gcaatagtct gctggacatc tgtgtctttg ggcgccgggc
     7021 gggtgtcagc gctgccttgt gggcgcaggg tgtccagccg g
//
LOCUS       DBNGSX010000501         4779 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_882610, whole genome shotgun sequence.
ACCESSION   DBNGSX010000501 DBNGSX010000000
VERSION     DBNGSX010000501.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4779)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_882610"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1069)
                     /gene="ygfZ"
                     /locus_tag="AC16CB_14855"
     CDS             complement(89..1069)
                     /gene="ygfZ"
                     /locus_tag="AC16CB_14855"
                     /inference="COORDINATES: protein motif:HMM:NF013720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CAF17-like 4Fe-4S cluster assembly/insertion
                     protein YgfZ"
                     /protein_id="HIR3343799.1"
                     /translation="MGTSELTGYQAAISSAAFYLQPKAGYLKVEGTDRIAFLQRQTTN
                     DLTRLALKGCLTSILTSPSARILDMFRVIDEGDSLGIITLAGHAESTLRYLKSRIFFM
                     DKVSVVDLSVEFAQIDLEGPGATKWLTELGIGIAPKLDEIVSVDMDGLPLRILGQPGL
                     SGQGYRLLVPAAAHPSLENTLNGLGIAHLEEEAYQALRVEAGLPAPGTELTEDYTPME
                     IGLDWVISDKKGCYTGQEVIARQITYDKITQQLVGLQLSSPAQPGERVWSDGKPAGKV
                     TSSARSPRFGEIALAVLKKPFHLPNTEVFVGSAPDLSSSAIVSVLPINSK"
     gene            complement(1138..3006)
                     /gene="ftsH"
                     /locus_tag="AC16CB_14860"
     CDS             complement(1138..3006)
                     /gene="ftsH"
                     /locus_tag="AC16CB_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR3343800.1"
                     /translation="MNTSRNRSSIIYLLLFVAIIAMVVYNFQQQATTQEVLAINQVAA
                     DIQSGIVERIVIESDNRLRIIRADGTEHISNKEDNATLVEQLKALGVTTEQLNSDRFK
                     IEIKPPSAWLGIATALGYILPFIILAGVFWFVFRQAQGSNNAAMSFGKSRARMFTGDQ
                     PSVTFEDVAGVDEAKEELEEVVEFLREPEKFISLGARIPKGVLLVGPPGTGKTLLAKA
                     VSGEAGVPFFSISGSEFVEMFVGVGASRVRDLFDQAKRHSPCIVFVDEIDAVGRQRGA
                     GLGGSHDEREQTLNQMLVEMDGFDTDTNVIIVAATNRPDILDPALLRPGRFDRRVILD
                     RPDMRGREEILKVHVKGKPLSTDVELGVLARSTPGFVGADLENLVNEAAILAARRNKK
                     MIGQPEMEESIERVIAGPERKSRLISPEEKRIVAYHEAGHAVVMNALPEADPVHKVSI
                     IARGMAGGYTLALPEEDRTLMPRKKMIADMIGLLGGRAAEELVFDDITSGASNDLERV
                     TRMARAMVTRLGMSNNLGPMVYGQKEELIFLGREISEQRDYSEAVAEEIDDEVRRLVS
                     EAYEKSKAILIEYRAKLDAVAHRLLEVETITRDEFESIFPAPVPKNSGTPVPQAVM"
     gene            complement(3156..4265)
                     /locus_tag="AC16CB_14865"
     CDS             complement(3156..4265)
                     /locus_tag="AC16CB_14865"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIR3343801.1"
                     /translation="MPRFAHVTGWGMAVPNKVMTNFELSKFVETNNEWIIDRTGIRER
                     RIANDDETTASLATDAALRALDMANLDPSKVDLVIVSTSSPEHLFPATACLVQDRIGA
                     SKAGAFDLSAACTGFIYALNFAAQAVRTGNVDNVLVIGAETLSRLVNWKDRGTCILFG
                     DGAGAFVLQATNVPGGVLSAVMRSDGSGGDLLSVPAGGSRLPTSAATIAQGQHYIQMN
                     GREVYRFATRVMTQATQEAVQKANLQMEDVNLIIPHQANQRIIEAAARQLNFPMDQVV
                     VNIGNYGNTSTASIPIATCEAFQKGQLNSGDRVVFVGFGAGLTWGAAVVQWSGPILAR
                     RRRLPRSYRAWARTRSFLNRLTRYIEGLLWGRWLK"
     gene            complement(4269..>4779)
                     /locus_tag="AC16CB_14870"
     CDS             complement(4269..>4779)
                     /locus_tag="AC16CB_14870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIR3343802.1"
                     /translation="QERGVPILAEFSGYGTTNDAYHISAPAENGAGAALCMRAALDDA
                     RLETGDIDYINAHGTSTPLNDKSETAAIKTVFGERAYNIPISSTKSMTGHLLGASGAL
                     EAVICVNALQTQSAPPTINYETPDPDCDLDYVPNSARSMKIDTVMSNSFGFGGHNASI
                     ILSRYLNGE"
BASE COUNT         1100 a         1244 c         1259 g         1176 t
ORIGIN      
        1 agtgtagggc gatggcgcgc ccaataccgc gtccggagcc ggtgatcagg gcgattttgc
       61 cagtaaacat caatactatt ttactggatt atttggagtt tattggcaat actgagacaa
      121 ttgctgagga tgataaatca ggcgcgcttc cgacgaatac ttccgtgttg ggtaggtgaa
      181 aaggcttctt tagtacagca agggcaatct cgccaaatct tggtgagcgc gcggaggagg
      241 ttaccttgcc agctggtttt ccatctgacc atactcgttc tcctggttgg gcaggtgaac
      301 tcagttgaag accaaccagt tgttgggtga tcttatcgta tgtgatctga cgggcgatta
      361 cttcctgacc agtgtaacac ccctttttat cggagataac ccaatccaag ccgatctcca
      421 ttggggtgta gtcctcagtc agctcagttc caggtgcggg caggccagct tccacgcgca
      481 atgcctgata ggcttcttct tccaggtgtg caattcctaa tccattgaga gtgttttcca
      541 aagatgggtg tgccgccgca ggtacgagaa ggcgatatcc ttggccgctt aagccgggct
      601 gtcccaggat gcgcaggggg agcccgtcca tatctacgct aacaatctca tccaatttag
      661 gggcaatccc aatgcctagc tcagtaagcc atttggttgc gcccggtccc tccagatcta
      721 tttgggcgaa ttcgacactg aggtcaacca cactgacctt atccatgaag aagatacggc
      781 tttttagata gcgaagcgtg gactctgcgt gtcctgcgag ggtgatgatt cctaaggaat
      841 ctccttcgtc aataacgcga aacatatcca ggatgcgcgc actgggggag gttagtatgc
      901 tggtgaggca acccttcaag gcgagccgtg tcaggtcgtt ggtagtctgc cgctgtaaaa
      961 aagcaatacg atcagttcct tcaaccttca gatagcctgc tttgggctgg agatagaacg
     1021 ctgcgcttga aattgccgcc tggtaaccag taagttcgga ggtacccatt gtagatggtg
     1081 gtaagtggag ttaatagctg atacgggtgc tggagcaccc gtatcactaa ttgtttgtta
     1141 catgaccgct tgaggcactg gcgtgccgct gttctttggc acgggtgcag ggaagatgct
     1201 ctcaaattca tcgcgggtta tggtctcgac ttccaacaag cgatgcgcta cggcatccaa
     1261 ctttgcgcga tactcaatga gaatggcttt ggatttctcg taggcttcgc ttaccagccg
     1321 acgcacttca tcatcgatct cctctgcgac cgcttcagaa taatcgcgct gttctgaaat
     1381 ctcgcggcca aggaaaatca gctcttcttt ctgaccatag accatcggtc ccaagttatt
     1441 actcatgccc agacgggtaa ccatggcgcg tgccatgcgg gttactcttt ccaggtcgtt
     1501 tgacgctcca gacgtgatgt catcaaatac cagctcttcg gcggcgcggc cacctaagag
     1561 gcctatcatg tctgcgatca ttttcttgcg cggcattagc gtcctatctt cctcgggtaa
     1621 ggctagtgta taacctccag ccatgccgcg ggcaatgatc gacaccttat gcacagggtc
     1681 agcttctggc aatgcgttca ttactaccgc gtgacctgct tcatggtagg ctacgatgcg
     1741 cttctcttcc gggctaatca atcgactctt gcgctccggt ccagcgataa cacgctcaat
     1801 ggattcttcc atctcaggct ggccgatcat tttcttgttc cgccgggcgg ctaagatggc
     1861 agcttcattg actagattct ccaggtcagc acccacaaat ccgggtgtag agcgggctaa
     1921 aacacccaat tcaacatccg ttgagagtgg tttgcccttg acatgtacct tgagaatttc
     1981 ctcgcgcccg cgcatatctg ggcggtcgag gatcactcgc cggtcaaagc gtccaggacg
     2041 cagcagagca ggatcgagga tatcgggccg gttggttgcg gcgacaatga tcacattggt
     2101 gtcggtgtcg aagccatcca tctcaaccag catctggtta agggtttgct cgcgctcgtc
     2161 gtggctgcca cccagaccgg cgccgcgctg gcgaccgact gcatcaattt cgtccacaaa
     2221 gacgatgcat ggggaatggc gtttggcttg gtcgaacaag tcgcggacgc ggcttgcgcc
     2281 tacaccaacg aacatttcaa cgaactcaga gccagaaatt gagaagaaag gtacgccggc
     2341 ttcaccggag accgcttttg cgagcagagt cttaccggta ccgggcggac cgactaggag
     2401 cacacctttc gggatgcgtg cccctaaaga gatgaatttc tcgggttcgc gcagaaattc
     2461 aacaacttct tccaattcct ctttcgcttc atcgacacca gcgacatcct caaatgtaac
     2521 cgagggttgg tcgccggtga acatgcgggc tcgggacttt ccaaacgaca ttgcggcgtt
     2581 attactgccc tgcgcctgcc ggaaaacaaa ccagaaaaca cccgccagga tgatgaaagg
     2641 caagatgtat ccaagtgcag tagcaatacc taaccatgca ctgggcggtt tgatctcgat
     2701 tttgaaacga tccgagttga gctgttcagt tgtaacgccc aatgctttga gttgttcaac
     2761 cagtgtggcg ttatcttcct tgttagaaat atgctctgta ccatctgcgc gtattattcg
     2821 caatcggtta tcgctttcaa taacaatccg ctcgacaatc ccgctctgaa tatcggcagc
     2881 aacctggtta atcgctaaaa cttcttgcgt ggttgcctgc tgctggaagt tatagactac
     2941 catggcgatg atcgccacaa acagcagcag gtatataata gatgatcggt tgcgggacgt
     3001 attcacttta cctccaaaat tatcagacaa tcccaattca ttagtttaaa ttataccaac
     3061 ctatggggat tgatgggagg agtaggggtg gcatttttag aaatctctaa caacattcta
     3121 acaaatttct cgacagatac gagtgattct ttggactatt tgagccatct tccccagagt
     3181 aacccttcaa tatagcgtgt tagccgattt aagaaggagc gcgtacgtgc ccaggcacgg
     3241 taactgcgag gcaagcgcct cctgcgcgcc aggattggtc cactccattg cacgaccgcc
     3301 gctccccagg tgagaccagc gccaaaacct acgaagacga cccggtcccc cgaatttaac
     3361 tgcccttttt ggaacgcttc acaggtagcg atagggatcg atgcggttga tgtgttgccg
     3421 taattgccaa tatttaccac cacctggtcc atggggaaat tgagctggcg agcagccgct
     3481 tcaatgatgc gctggtttgc ctgatggggg atgattagat taacatcttc catttgcaga
     3541 ttagcctttt ggacagcttc ttgcgttgcc tgagtcatca cccgagtggc aaaacgatag
     3601 acctctctgc cattcatttg gatatagtgc tgaccttgcg caatggttgc cgcactggtt
     3661 ggcaatcgac taccgcctgc aggtactgaa agcagatcac ctccggaacc gtctgaccgc
     3721 atcactgcgg agagcacacc ccctggcaca ttggtggctt gcaacacgaa ggcccccgct
     3781 ccatcgccaa ataggatgca agtgccgcga tccttccagt tcactaggcg cgagagtgtc
     3841 tccgcgccga tgaccaggac attatctaca ttgccggtac gtactgcttg ggcagcgaaa
     3901 ttgagggcat agatgaagcc cgtgcaggca gctgagaggt caaaagcccc ggctttgctg
     3961 gcgccgatgc ggtcttggac gagacaggca gtggcaggaa agagatgctc tggcgatgaa
     4021 gtggaaacga tcaccaaatc aactttggag ggatccaggt ttgccatatc cagagctcgc
     4081 agggctgcat cggttgccag ggaggcggta gtctcatcat cattagcgat ccgcctttcg
     4141 cgaatgcccg ttcgatcgat tatccattcg ttgttggttt ccacaaattt ggatagctcg
     4201 aaattggtca tcaccttatt tggaacagcc atcccccaac ctgttacgtg ggcaaagcgt
     4261 ggcatcttct attctccatt taaataccga ctaaggatga tggaggcatt gtggccgcca
     4321 aatccaaatg aattagacat caccgtgtcg attttcatcg accgggcaga attagggaca
     4381 taatccagat cgcaatctgg gtctggtgtc tcatagttga tggtgggagg ggcagactgg
     4441 gtctgaaggg cattgacaca gatcacagct tccagtgcgc cggaagcccc cagcaagtgg
     4501 cccgtcatcg atttggtaga ggaaatcggg atgttataag ctcgctctcc aaagacggtc
     4561 ttgatggctg ctgtctcact cttatcgtta aggggggtgc tggtgccgtg agcgttaata
     4621 tagtctatat cgccggtttc taacctggca tcatctaggg cggcgcgcat gcataaggcc
     4681 gcaccggcgc cgttctctgc aggcgcagag atgtgatagg cgtcattcgt ggtgccatag
     4741 ccagaaaact cagccaggat gggcacccca cgttcttgg
//
LOCUS       DBNGSX010000502         4220 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1850971, whole genome shotgun sequence.
ACCESSION   DBNGSX010000502 DBNGSX010000000
VERSION     DBNGSX010000502.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1850971"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="AC16CB_14875"
     CDS             complement(<1..511)
                     /locus_tag="AC16CB_14875"
                     /inference="COORDINATES: protein motif:HMM:NF014713.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HIR3343803.1"
                     /translation="MVISNITLGLAFLAGLASFLSPCVFSLVPAYVGYLGGRTVSSAK
                     SEAPSRWVTFSHGLAFVLGFSVVFILLGVAASALGGVLYDLRTWVAKIGGIVVIVFGL
                     HMTGIFRIPFLEYDLRPQSTPDRRQGYAASALMGVFFSAGWSPCVGPVLGAILTFAIN
                     GGSIAHGVSM"
     gene            complement(541..1641)
                     /locus_tag="AC16CB_14880"
     CDS             complement(541..1641)
                     /locus_tag="AC16CB_14880"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIR3343804.1"
                     /translation="MRKEAITRRNFLHLAALSLGGFALRPLRRIAYLQDFPKYERLGR
                     AFTKVEIKARPDINSQTVGVIYDDAVVPWLREVSGYHPYRYKQRFVETIDGYIWASDL
                     QPVRNLPNQPLQTIHETSLGPGMWAEVSVPWVDIVLENNPASPGFKARLEVGMPLRLY
                     YGQVLWIDKVKSDESGQVWYRVNERYGYGDLIWAAAEAFRPLTHDEVAPISPEVEDKH
                     IVVNVEEKYQTLACYEGKTEVYYCRISGGKKFDPDGTPLGHSSTPTGTHTIWRKQVSA
                     HMSGGTTGAGYDLPGIGWTTLFSGDGVAVHATFWHNNFGGELMSHGCVNATPEDAKWV
                     FRWAHPPVYYDPGDLYSKDTDIPPTKVTVLEG"
     gene            complement(1833..>4220)
                     /locus_tag="AC16CB_14885"
     CDS             complement(1833..>4220)
                     /locus_tag="AC16CB_14885"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140358 - P-type transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR3343805.1"
                     /translation="KVQGVQQASVNLSSERASVEYDPSLANLGDLIARVQKAGYGVAS
                     GEADLLIRRLSDDNDARRLETSLQKVDGVLEAQVNFASERARLSYIPTIISQSEIRSA
                     VAAAGFEAVETGGEVEDAERKARQAEIAQQRQYLIVGLIFTIPLFLFAMGRDLNLLPH
                     WAHDPWANWMMLALATPVQFYVGWQYYVGAFKALRNGSANMDVLIAMGSSAAYFYSLP
                     IVFGLISGHVYLETAAVIITLIKLGKFLEARAKGRTSEAIKKLMGLRAKTARIVRDGQ
                     EMEIATEEVRVGDIVIVRPGEKIPVDGVVVEGRSSIDESMLTGESLPVEKRPGDNVIG
                     ATLNKLGLLRFEATKVGRETALAQIIRMVEEAQGSKAPIQKLVDRVSAVFVPAVIVIA
                     LFTFVIWYFFFPLPPASDVDAFTRALINMVAVLVIACPCAMGLATPTAVMVGTGKGAE
                     MGVLLRSGEALERAGKITMVVLDKTGTITKGQPAVTDIIEGGGSKGNKGQPSIPTHGF
                     TSSSLEMLRLAASVEQGSEHPLGEAIWAEANNKALQLSPADAFSAEVGNGVQAEVDGH
                     TVLVGNLRMMEGRGIALNGLQTEVTRLQNEAKTAMLIAVDGEVRGVIGVADTVKEGSP
                     EAIQALHQMHLKVAMITGDNQKTAEAIARKVGVDRVLAEVLPGDKVAEVKKLQESGEV
                     VAMVGDGINDAPALAQADVGIAIGTGTDVAMAAAAVTLMSGDLRGVPKAISLSQRTLR
                     TIKQNLFWAFFYNVILIPAAAAGLLNPMLAAGAMAFSSIFVVSNSLRLKGFKTEI"
BASE COUNT         1089 a         1212 c          957 g          962 t
ORIGIN      
        1 gcatacttac cccatgcgca atcgaaccac cgttgatcgc gaaggtcaat atcgcgccga
       61 gcacgggacc aacgcatgga gaccatcccg ccgagaaaaa tacgcccatc aacgccgagg
      121 ctgcatatcc ctgtcttcga tctggggtgg attggggtcg cagatcatac tccaagaacg
      181 ggatgcgaaa aattccagtc atatgcaagc caaatacgat cacgactatt ccaccaattt
      241 tagccaccca ggtacgcaga tcatacagaa ccccacccaa ggctgatgcc gccacgccca
      301 agagaataaa aactacgcta aaacccagga caaaagctaa cccgtggctg aatgtcaccc
      361 agcgacttgg cgcttcggat tttgccgatg agaccgtcct gccccctaaa tacccgacat
      421 aggccggaac aagcgaaaaa acacatggtg agagaaatga tgctaaacca gctaagaacg
      481 ccagcccgag agtaatgttc gaaatgacca tattggaaat tcaactccta gcaaaattat
      541 ttacccctcc aaaaccgtca ccttggtagg aggaatatcc gtgtccttac tataaagatc
      601 accagggtca taataaactg gtggatgggc ccaacggaaa acccatttcg cgtcttcagg
      661 ggttgcattc acacaaccat gagacatcag ttccccacca aagttattat gccaaaacgt
      721 agcatgaaca gccacaccat cgcctgaaaa gagcgtagtc cagccgatgc caggcaaatc
      781 atacccggct ccggtggtgc cgccactcat gtgggcagag acttgcttgc gccagatggt
      841 gtgcgttccg gttggcgtgg aggagtgccc cagtggagta ccatcgggat caaacttttt
      901 gccaccagat atccgacagt agtacacttc ggttttaccc tcatagcatg ccagggtctg
      961 atatttttcc tccacattta caacgatatg tttatcttca acctcagggc ttattggtgc
     1021 tacttcgtca tgtgtcaatg ggcgaaatgc ttctgcagcc gcccaaatca aatcgccata
     1081 cccgtagcgc tcgttcaccc ggtaccatac ctgcccgctc tcgtcactct tgaccttatc
     1141 gatccataaa acctgaccgt aatataatct caatggcata ccgacctcca gccgcgcctt
     1201 aaatccaggg ctggccgggt tattctccaa gacaatatct acccagggca cactcacctc
     1261 cgcccacatc cccggaccca ggctggtttc atgtatcgtt tgaaggggct ggttaggaag
     1321 gttgcgcaca ggctgcaaat ccgacgccca aatgtaccca tcaattgtct cgacaaagcg
     1381 ctgtttatag cgatatggat ggtacccgga tacctcgcgt agccaaggta ccaccgcgtc
     1441 gtcatagata acaccaaccg tctgcgaatt gatgtctgga cgagccttaa tctccacttt
     1501 tgtaaacgca cgccccagcc gctcatattt tggaaaatct tgcaggtagg cgattcgcct
     1561 gagtgggcgc agggcaaacc cacccaagct tagagcggct aaatggagaa aattgcgcct
     1621 ggttattgct tctttacgca cggggttaag cataatcgga aaagttaaga gggtcaatag
     1681 aaggaaatta caaccacatt aataaatgag atagggtcag gatagagttt cttacttatc
     1741 aggtatataa attatggatt gatggccaga caaatgcgac gagcagatta aaaatcaata
     1801 tgcggcgcat ccaaggatac gtcgcatatt aattatatct cggtcttaaa acccttcaag
     1861 cgcaggctgt tgcttaccac aaatatgcta ctaaacgcca tagcgccggc agccaacatg
     1921 ggattgagta ggccagcggc tgcagcaggg ataagaatta cgttatagaa aaaggcccaa
     1981 aaaagatttt gcttgatggt gcggagtgtg cgttgtgaca gcgaaatcgc ttttggaacg
     2041 ccgcgcaggt caccgctcat caaagtcacc gcagcagcag ccatcgccac atcggtgccg
     2101 gttccaatag caatgcccac atcggcttga gctaaggcag gtgcatcgtt gattccatca
     2161 cctaccatag ctacaacctc gccgctttcc tgcagtttct ttacctcggc aaccttatct
     2221 ccaggtagca cctcggccaa cacgcgatcc acgccgactt ttctggcaat cgcctctgcg
     2281 gttttttggt tgtcaccagt gatcatagca accttcagat gcatttgatg taaggcttgg
     2341 attgcttccg gtgagccctc tttcacagtg tccgctacac cgatcacccc tcgcacctct
     2401 ccatccaccg caatcagcat ggcagtcttg gcttcattct gtagccgagt gacctcggtt
     2461 tgtagaccat tcaaagcaat tccgcgccct tccatcatgc gcaagttacc gaccagtacg
     2521 gtatgcccat ctacctcagc ttgcacgcca ttccccacct cagcactgaa ggcatcggct
     2581 gggctaagtt gtagagcctt gttatttgct tctgcccaaa tagcttcccc tagggggtgt
     2641 tcacttccct gttctacaga agcagccagc ctaagcatct ctaacgaaga agacgtaaaa
     2701 ccgtgagtgg gaatgctggg ttgacccttg tttcccttag aacctccacc ttcgataata
     2761 tcggttaccg ctggctggcc tttggtgatc gtgcctgttt tgtccaatac caccatagtg
     2821 attttacctg cacgttccag cgcctcacca gagcgcaaca gcacacccat ttctgcacct
     2881 ttgccagtac ccaccatgac tgccgtcggt gtggctaagc ccattgcaca cgggcaggca
     2941 attaccagca ctgccaccat gttaatcagg gcgcgggtaa acgcatccac atccgaggcc
     3001 ggtggaagtg ggaaaaagaa gtaccatatg acaaaggtaa acagagcaat gacaatcacc
     3061 gcggggacaa acactgctga aacccgatcg actaatttct ggatcggcgc tttactgccc
     3121 tgggcctctt ctaccatacg gataatttgt gccagagctg tctcccgtcc tactttggtg
     3181 gcttcaaatc tgagcaatcc cagcttgttt aacgtcgcac caatcacatt atccccaggt
     3241 cgcttttcaa ccggcaaaga ttcgccagtc aacatagatt cgtcgatact tgagcgtcct
     3301 tcgaccacaa cgccatccac ggggattttt tcacctgggc ggacgatcac aatatcacca
     3361 acacgcacct cttccgtggc gatctccatt tcctgaccat cccgcacaat ccgggcagtt
     3421 ttggcgcgca gacccattaa tttcttgatc gcctcgcttg tccgaccttt tgcacgcgcc
     3481 tctaaaaatt tacccaactt aatcagggta atgatcacgg cagcggtttc cagatagaca
     3541 tgtccgctga tcaatccgaa cacgatgggt agtgaataga aatacgcagc tgaagacccc
     3601 atggcgatga gcacatccat attggcggat ccattacgca gcgccttgaa tgcacccaca
     3661 taatactgcc agccaacata aaactgcacc ggagtagcca gtgctaacat catccaattc
     3721 gcccatgggt cgtgcgccca gtgaggaagc aggttaagat cccgacccat ggcaaacaaa
     3781 aagagcggga tggtgaatat taatcccaca atcaagtact ggcgctgctg ggcaatttcc
     3841 gcttggcgtg ccttgcgctc agcatcctcc acttccccac cagtctcaac tgcctcaaaa
     3901 cctgctgcgg caaccgcgct gcgaatctcc gattggctga ttatcgtagg gatatagctc
     3961 aagcgtgcgc gctcgcttgc aaaatttacc tgggcttcga gaacgccatc taccttttgc
     4021 agggaggtct caaggcgacg agcgtcgtta tcatcactca aacgccgaat aagcaagtct
     4081 gcttcacccg aggcaacccc gtagcctgct ttttggacgc gggcaatgag gtctccaagg
     4141 ttcgctagcg aaggatcata ctcaacgctc gcgcgctcag aagacagatt gacggatgcc
     4201 tgttggacgc cttgtacctt
//
LOCUS       DBNGSX010000503         7736 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_119965, whole genome shotgun sequence.
ACCESSION   DBNGSX010000503 DBNGSX010000000
VERSION     DBNGSX010000503.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7736)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7736)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7736
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_119965"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="AC16CB_14890"
     CDS             complement(<1..989)
                     /locus_tag="AC16CB_14890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048039 - ubiquinone binding [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 4 family protein"
                     /protein_id="HIR3343806.1"
                     /translation="MDFIASHLLTLILFTPVLAAVILFFLPREQEDLLRWTAFVLSFV
                     PLAFSLALWFGFDAGKPGFQYEERAVWYAAINSSYHLGVDGISLTMVLLTTLLTPLAI
                     LASFTIKDNIKAYMILFFLLEMGMLGVFLSLDLLLFFVFWEFGLVPMYFLISQWGSEK
                     GERQIWGGRTVSARAYASFKFMIYTMAGSLGLLLAIQVIGVTARTYDMTQLFQIWPGL
                     QSLNDLPLNLPVSTVKAVAFWAFAIAFAIKVPVWPFHTWLPDAHTEAPTAGSMILAGV
                     LLKLGAYGFLRLVLPLYPVEAKYYAGALGFLAVMAIIFGAFASYGQDDFKRLVA"
     gene            complement(1022..3196)
                     /gene="nuoL"
                     /locus_tag="AC16CB_14895"
     CDS             complement(1022..3196)
                     /gene="nuoL"
                     /locus_tag="AC16CB_14895"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HIR3343807.1"
                     /translation="MATELLIWLIPLPPLLAFFLIVLFTNRSKWLSHTVAVGAALLSW
                     LGSMVVFFRSLQVEHFGEKIFASSIPWLPTGDSWFRIGVQIDPLSAATLFFVAWTVLM
                     IFLYSVGYHNFGQPKGDHDVAGLPPHGATVSDAHGHTHKVPSIEPMYSRFFAFIGLFA
                     FGMYTLVVSDNLLTLFVGWEIMGLCSYLLIGFWYGKPSARAAMIKAFITTRVGDVFML
                     LGIAYLYSVAGTLNYREIFYNEEMLAHLAEMPSYIVGLSAAGLIGILLFIGTIGKSAQ
                     WPLHVWLPDAMEGPTPVSAMIHAATMVSAGVYMAIRIFPLLSAGSEHGATSAMMLVSF
                     IGAFTALFAATIAVAQNDIKRVLAYSTISQLGYMVAALGIGAYVAAAFHLITHAFFKA
                     LLFLGSGSVIHGMEHGVLHTGDHIDPQNMFNMGGLRKKMPVTFWTFLIGGFALSGFPL
                     VTAGFWSKDEILADAWGHGHVVVFGTLALAALLTAFYTMRQITLTFLGKPRTEAAHHA
                     QETPWTMTAPLAILAVFSVAAGWVGIPEHFPVLGGLLPNWFHEFLGGTLLEHPEAVEF
                     NLVPLLTSLVVALGGLYMGWLVYRETPAGAQDPIEVTVPSMHKLLQNKYYFDEIYDRI
                     FVRPAYWFSETFTSAWMDQGVIDGVLHWVAMVSGTIGGTLREYIDKRIVNASGDIVGE
                     GIKKFGRAFRVIQTGRIQQYMIGALVALAFFSALFYYLIFTP"
     gene            complement(3206..3514)
                     /gene="nuoK"
                     /locus_tag="AC16CB_14900"
     CDS             complement(3206..3514)
                     /gene="nuoK"
                     /locus_tag="AC16CB_14900"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HIR3343808.1"
                     /translation="MIPLSWFLILSAALFSVGLFGVLARKNAVAILMGVELMLNAVNI
                     NLVAFWRYRTPELIAGQAFAIMVFAVAAAEVAVGLALIISIYRRRNTVVADDIDMLKL
                     "
     gene            complement(3518..4027)
                     /locus_tag="AC16CB_14905"
     CDS             complement(3518..4027)
                     /locus_tag="AC16CB_14905"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J family
                     protein"
                     /protein_id="HIR3343809.1"
                     /translation="MTPLQIVFILVAAATLGSALLVVTTRNLVHAALWLVASLFGVAV
                     VYVLLNAGFLAVAQVVIYIGAIAILMIFAIMLTRRVATDTGPQINSNWGWAAILSAVI
                     FAFLAWMLGSWPGFSTALPDMSPRSDPLRELGQALVSPDLYVIPFEVASVLLLAALIG
                     AIMVAWERK"
     gene            complement(4043..5278)
                     /gene="nuoH"
                     /locus_tag="AC16CB_14910"
     CDS             complement(4043..5278)
                     /gene="nuoH"
                     /locus_tag="AC16CB_14910"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HIR3343810.1"
                     /translation="MGDPVTFIAIWLKGLLLGWGLAPGFVTLILAVIGVVVMATVALV
                     LDIFLVWVERKVVARFQDRLGPNRLGPFGLVQPVADIIKLLIKEDITPIGADRIVYNL
                     APIMALGTVLTLWAVLPISATIYGSNINVGVLYIVAIGAISTLGIIMAGWASNNKYAL
                     LGAFRTVAQMVSYEVPLVITLLVPVILARSMGLKDIVDGQTVWFLFMAPLAALIFLVS
                     NIAELGRAPFDLTEAESEIVAGFHIEYTGMKFGLFYAGELLHALTVGAIFSTLFLGGW
                     RGFGAETYPIIGVFWFFLKAFFMYWVIMWVKYTFPRIRIDHMLNFNWKFLTPLSLVVL
                     IVTAIADKLVPRDNLLVYTLWMLLANIVIAWFTLQILRARARVERKTVGEPRPVATPP
                     QQPNVMPSSASQPPAGTAQ"
     gene            complement(5363..5719)
                     /locus_tag="AC16CB_14915"
     CDS             complement(5363..5719)
                     /locus_tag="AC16CB_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR3343811.1"
                     /translation="MLNDWLFIGVFLLLAPIFPGLALVVPRLIAPRKPNPIKLQTYEC
                     GIETVGDSWVQFRVQYYIFALVFLIFDVETVFLYPWAVAFDQLPLFAVLEGVLFILIL
                     VAGLIYAWRKGVLEWI"
     gene            5954..>7736
                     /locus_tag="AC16CB_14920"
     CDS             5954..>7736
                     /locus_tag="AC16CB_14920"
                     /inference="COORDINATES: protein motif:HMM:NF013961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HIR3343812.1"
                     /translation="MKITRPPGTSRWWDIPSILILLVILTTAFTRLSATNWSENLDAI
                     RMISYVGLLAGLALGYSRFSPRQVAFIALAYGIFTIPWRVGLSLGEEIAWMERLQSMA
                     GRLGVILWYLLQQRAAPDNFLFVTLMSILFWFTSVHAGYTLVRYANPWVVALPSGVAI
                     VLIQTYDPHPRRVWGLIIYLFLALLLVARLDFVNRRKRWEQNHTYIPSYLGTDFLRFA
                     LVACLLLIALSWATPALAKALPAAKTSWDRVVTPWWNDVRNVFENAFSSLRSSIGLTG
                     EYYGPNLSLGRGNILSDTVIFTVQAPGGIPAGVRYYWRARVYDTYEKIWISSLQTTRA
                     LDAQEIDLKFPDLSDNPPRTPSFTFYLSRPLATILAPNHPVWLSRPSRAEFSLNPDGT
                     VDLGMLRATPSLRGGEIYSVRSSFNVVTVEELRQAGTDYPSWVTARYLQLPTSITIRT
                     RQLAAQIAENKETPYDRAQAITQYLRENYQYTETVPALPSNQELIDWFLFDLKQGFCN
                     YYATAEVVLLRSLGIPARLAVGYASGENVDDPEFYTVRQLDAHAWPEVYFPEIGWVEF
                     EPTVSQPELSRPLTALGQDNLNNLSSSN"
BASE COUNT         2098 a         2076 c         1917 g         1645 t
ORIGIN      
        1 gcaaccagcc gcttgaaatc atcttgcccg taggaggcaa aagccccaaa gataatcgcc
       61 atcaccgcca ggaaacccag cgctccggca tagtacttcg cctcaacggg ataaagcggc
      121 agcaccaagc gcaggaaacc ataagcaccc agttttagca ggacacccgc caggatcatc
      181 gagccggcgg taggagcttc ggtgtgggca tcaggcagcc atgtgtggaa gggccacacg
      241 ggcactttga tggcgaaagc aatggcaaat gcccaaaagg ccaccgcctt gaccgtagaa
      301 accggcaggt tgagcggtag atcgttcaaa gattgcaagc ccggccagat ctgaaatagc
      361 tgggtcatat cgtaggttcg agcggtcaca ccgatcactt ggatcgccag cagaagtccc
      421 aaagagcccg ccatggtgta gatcatgaat ttgaatgagg cgtaggcacg ggctgagacc
      481 gtacgcccgc cccaaatctg gcgttcgcct ttctcgctgc cccactggct gatgaggaag
      541 tacatcggta ccaggccaaa ctcccaaaag acgaaaaaga gcagcagatc cagggataga
      601 aagacgccca acatgcccat ctcgagcagg aagaacagga tcatatatgc tttgatattg
      661 tctttgatgg taaaggatgc caggattgcc agtggagtga gcaaagtagt cagcaagacc
      721 atggtcaggg aaatgccgtc caccccaagg tgatacgaag agttgatcgc cgcgtaccag
      781 acggctctct cctcgtattg gaaaccgggc ttcccggcat caaacccgaa ccacagggct
      841 aaggaaaatg ctaggggtac aaagctgagc acaaatgctg tccagcgcag caggtcttcc
      901 tgctcgcgcg gcaggaaaaa caggatcact gccgccagca ccggtgtgaa caagataagt
      961 gtaagcaagt gacttgcgat aaaatccatc gttcgctcct gtaggtccga tgtgacgaac
     1021 gtcagggggt aaagatcagg taatagaaca acgcgctaaa gaaggcaagc gcgaccaatg
     1081 ccccaatcat gtactgctga atgcgcccgg tttggatgac gcggaatgca cgaccaaact
     1141 tcttaatgcc ctcacccaca atatcaccag aggcattcac aatgcgctta tcgatgtatt
     1201 cgcgtagcgt gccgccaatc gttccggaga ccatcgccac ccagtgcaaa acgccatcga
     1261 tcacaccttg atccatccaa gctgaagtaa atgtctctga gaaccaatag gctgggcgca
     1321 cgaaaattcg atcatagatc tcatcaaagt agtacttgtt ttgcaagagc ttgtgcatac
     1381 taggcacagt tacttcgatt ggatcttgcg ccccggcagg tgtttcccgg taaaccaacc
     1441 agcccatata caatccgccc agcgcaacga ccagcgaagt gagtagtggg acaaggttga
     1501 attcgaccgc ttctggatgc tccaacaaag ttcctccaag gaactcgtgg aaccagttag
     1561 gcagtagtcc cccgaggact gggaaatgct ctgggatgcc cacccacccg gctgcaacag
     1621 aaaataccgc caggatagcc aatggtgcgg tcatcgtcca gggagtctcc tgagcatggt
     1681 gggcagcttc ggtgcgcggc ttccccaaga aggtcagggt gatctggcgc atggtgtaaa
     1741 aagccgtgag caatgccgcc agtgctaagg tgccaaagac cacaacatgc ccgtgtcccc
     1801 aggcatctgc caggatttca tcttttgacc agaatccggc tgtcaccaaa ggaaagccgg
     1861 agagcgcgaa gccaccgatc aggaatgtcc agaaagtcac cggcatcttc ttgcgcaggc
     1921 cgcccatgtt gaacatattc tgcgggtcga tgtggtcgcc ggtatgcagg acaccatgct
     1981 ccatgccgtg tatcaccgag ccagagccca agaagagcag tgccttaaag aaggcatgcg
     2041 tgatcagatg gaatgctgcc gccacgtaag cgccgatacc cagggcagcc accatatacc
     2101 ccagttgcga aatcgtcgaa tatgccagca cgcgcttgat atcgttttgg gcaaccgcga
     2161 tggtggcggc aaataaggca gtgaacgccc cgatgaacga caccaacatc atggctgagg
     2221 tcgccccatg ctcggatcct gcagagagca gcgggaagat gcgaatcgcc atgtagacgc
     2281 ccgctgaaac catcgtggca gcatggatca ttgctgagac cggcgtaggg ccttccatcg
     2341 catcgggcaa ccatacgtgc aaaggccact gggcagactt gcctatcgtg ccgatgaaca
     2401 gcaggatacc aattaacccg gctgctgata gacccacgat ataggagggc atctcagcca
     2461 ggtgtgccag catctcttcg ttataaaaaa tctcacgata gtttaacgtg ccagccaccg
     2521 agtacaggta ggcgatgccc aacagcataa agacatcacc aacgcgcgtg gtgataaatg
     2581 ccttgatcat cgctgcccga gcagatggct tgccgtacca gaaaccaatc aacaggtacg
     2641 agcacagtcc catgatttcc cagccaacga agagggtaag caggttatca gagaccacca
     2701 gcgtatacat gccaaaggcg aagagaccga tgaaggcaaa aaagcgtgag tacatcggct
     2761 caatggatgg gactttgtgc gtgtgcccgt gcgcatcgct aaccgtagcg ccatgcggtg
     2821 gaagaccggc aacgtcatgg tcacccttgg gttggccaaa gttgtgataa cccacgctgt
     2881 acaggaagat catcagcacg gtccaggcga cgaaaaacag ggttgctgca gagagcggat
     2941 caatttgcac gccaatcctg aaccacgaat cacccgtagg aagccagggg atggaggagg
     3001 cgaagatttt ctctccaaag tgttcaacct gcagcgagcg gaagaacacc accatgctgc
     3061 ccaaccagga gagcaacgct gctccaacag cgactgtatg gctgagccat ttgctacggt
     3121 tggtgaacag gacaatcagg aaaaatgcca acagcggcgg gagggggatt agccagatta
     3181 ataactcagt tgccatgtat tctccctaaa gtttgagcat atcgatatcg tcggctacta
     3241 cggtgttgcg ccgccgatag atagaaatta tcaacgccag gccgacagct acctcagccg
     3301 ccgctacggc gaacaccatg attgcaaatg cttgtccggc aatcaattcc ggcgtgcgat
     3361 aacgccagaa agcaaccagg ttgatattta ccgcattgag catcagctct actcccatca
     3421 agattgccac agcgtttttc cgcgccaaaa caccaaaaag gccaacgctg aagagagctg
     3481 ccgatagaat aaggaaccag gaaagaggga tcatgcttta cttcctttcc caagccacca
     3541 taatggctcc aatcagtgct gccagcagta aaactgaagc cacctcgaag gggataacgt
     3601 ataaatccgg ggagaccaaa gcctgtccta attccctgag tgggtcggag cgcgggctca
     3661 tgtcaggcaa ggcagtagaa aatccaggcc aacttcccaa catccaggcc aagaaggcaa
     3721 atataactgc ggacaggatt gctgcccaac cccagttgct attgatctgt gggcctgtat
     3781 cggtggctac ccggcgagtg agcatgatgg cgaagatcat caagattgca atcgctccaa
     3841 tatagatgac cacctgtgcc actgccaaaa aacccgcatt aagcagcaca tatacaaccg
     3901 cgaccccaaa tagggatgca actaaccaaa gagcagcatg cactaggttg cgcgttgtga
     3961 ctacaagcag cgcagagcct aaggttgccg cagcgacaag gataaaaaca atttgcaaag
     4021 gagtcatgga cacactccac ttctattggg ctgtccctgc gggtggctgg gaagcactcg
     4081 atggcattac gtttggttgc tgaggtggtg tcgccacagg tcgcggttct cccacggttt
     4141 ttcgttcaac ccgtgctcgg gcgcgcaaga tttgcaaggt aaaccaagca atgacaatgt
     4201 ttgccagcag catccacagg gtgtagacga gcaaattatc gcgaggcact aatttgtcgg
     4261 cgatggcagt cactatcaag accaccagtg aaagaggtgt aaggaatttc caattaaaat
     4321 tcaacatgtg atcaatacgt atgcgcggga aggtgtattt gacccacatg atcacccagt
     4381 acataaagaa agctttgagg aaaaaccaga acaccccgat gatcggataa gtctctgcgc
     4441 caaatccacg ccagccacca aggaaaagag tagagaatat tgctccgacc gtcaaggcgt
     4501 gcaacagctc tccagcgtag aacaaaccga atttcattcc agtgtactcg atgtgaaatc
     4561 cagcgacgat ctcggactct gcctcggtca ggtcaaacgg cgcccgccct aattctgcga
     4621 tgttggacac caggaaaatt agagctgcta gtggcgccat gaatagaaac cacacggttt
     4681 gaccatcaac aatatcctta agccccatgg agcgggccaa tatcacaggt accagcaaag
     4741 tgatcaccaa gggaacttcg tatgaaacca tctgcgctac agtacgaaat gcgcctagta
     4801 gggcatattt attattcgaa gcccaacccg ccatgataat tcccagcgtg cttatcgcac
     4861 cgatagcgac aatatatagc acgcccacat tgatattgct gccatatatt gtggctgaga
     4921 tgggcagtac tgcccacaag gtaagcactg tgcccaaggc cataattggg gcgaggttat
     4981 agacaatcct gtctgcacca atcggggtga tgtcctcttt aataagcagc ttgatgatat
     5041 cggcaaccgg ttggaccaat ccaaatggcc ccagtcgatt tggtcccaga cgatcctgga
     5101 aacgggcaac tacctttcgt tcgacccata ccagaaaaat atccagcacc aaagcaactg
     5161 ttgccattac aactacaccg atcactgcta agattaaggt gacaaaacca ggggcaagcc
     5221 cccacccaag cagcaggcct ttcaaccaaa tggcgatgaa tgttactgga tcacccatcc
     5281 aaaaactcct tatcctaaac acgtaaaagg ctgagggaga tacccatcag cctttttata
     5341 cattagcatg accaaaattt ctttagatcc actccaacac gcctttgcgc caagcatata
     5401 tcagcccagc gaccaagatc aagataaaaa gaacgccttc caggaccgcg aacaatggca
     5461 actggtcgaa ggctactgcc catggataca gaaagacggt ctcgacatcg aaaatgagaa
     5521 ataccagagc aaaaatatag tattgtacgc ggaattgtac ccaggaatcc cccaccgttt
     5581 cgataccgca ttcataggtc tgtaatttta ttgggtttgg cttgcgaggc gcaatcaacc
     5641 ggggaaccac cagggctaac ccgggaaaaa taggggcgag caacaaaaat acgccgatga
     5701 ataaccagtc gttgagcatg caggctcctt atttagaagt atacgcctat tccccaagaa
     5761 agtgagaagg agaacaacgg cgcaaatttt accataagtt caactgtttt tacaaagttt
     5821 gttccatttt ttacagattg tggatctgta ttcctgatta atgctttcaa aacaggtaat
     5881 ggagcaatgt taattgtaca tcgaaaataa ctatcgaacc aactaccgct ttttttggta
     5941 aactgaatga aagatgaaaa ttacgcgccc tcctggaaca tcccgttggt gggatatacc
     6001 gtcgattctc attcttttgg tcatattgac cacagccttt acgcgcctat cggcaaccaa
     6061 ttggtctgag aacctcgatg ccattcgcat gatttcctac gttggattgc ttgctggatt
     6121 ggctttgggg tacagccgct tctcgccacg ccaggtagcc ttcatcgccc tggcttacgg
     6181 tatctttacc atcccctggc gcgttggtct ctctctgggc gaggagattg catggatgga
     6241 acgattgcaa agcatggctg gacggttagg tgtgatcctt tggtatttac tccagcagag
     6301 agccgcgccc gataactttc tatttgtgac cttgatgagc atcctcttct ggttcaccag
     6361 cgtgcacgct gggtatacgc tggtgcgtta cgccaaccct tgggtggtag ccctacccag
     6421 cggtgttgca attgtgctta tccaaaccta tgatccgcac ccgcgccgtg tctggggtct
     6481 aattatctat ttgtttcttg cgcttttact agttgcccgc ttggattttg ttaatcgacg
     6541 gaagcgctgg gaacagaacc atacctatat accatcgtat cttggaacgg atttccttcg
     6601 cttcgccctg gttgcatgcc ttctcctaat cgcactttca tgggcaaccc cagcacttgc
     6661 aaaggcactc cccgctgcta aaacgagctg ggatcgagtg gtgactccct ggtggaacga
     6721 tgtgcgaaat gtatttgaga acgcattttc ctcactccgc tcctcgatag gcctgacggg
     6781 ggagtattat ggcccgaatc tctcactagg tcgtggcaat attctgagcg atacagttat
     6841 attcaccgtc caagcgcctg gtggtatacc cgcgggggtg cgctactatt ggcgagcgcg
     6901 cgtctacgac acgtatgaga aaatctggat aagctcgtta caaaccacac gcgccttaga
     6961 cgcccaggag attgacctta aattccctga tctctcagat aaccctccac gaacgccctc
     7021 ctttaccttc tatctgagtc gcccattggc tactatctta gctcccaacc atcctgtatg
     7081 gttgagtcgc ccttcacgcg ccgaattctc cctcaatccc gatgggactg ttgatctagg
     7141 catgctacgc gccacgcctt ccttgcgtgg cggcgagatt tatagcgtac gttcttcatt
     7201 caatgtagtt actgtggaag aattgagaca agctggcaca gattacccca gctgggtcac
     7261 agcacgctat ctgcaattac ccacgtctat tacaatacgc acccgccagc ttgccgccca
     7321 gattgcggag aataaagaga caccctatga tagagcccaa gccatcacgc aatatttgcg
     7381 tgaaaactat caatacactg agaccgttcc ggccctgcca tccaatcaag aactgatcga
     7441 ctggttctta ttcgatctaa agcagggatt ctgtaattat tacgccacag ccgaggtcgt
     7501 cctgctccgc tctctcggta ttccagcccg ccttgctgta ggttatgcca gtggtgagaa
     7561 cgtggatgat ccagaatttt atacggtgcg ccagctagat gcccacgcct ggccggaagt
     7621 ttactttccg gaaatcggtt gggttgaatt cgagccaaca gtttcccaac cagaattatc
     7681 tcgtccatta acggcgcttg gacaagataa tctaaataat ttatccagct cgaatc
//
LOCUS       DBNGSX010000504         7471 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1782281, whole genome shotgun sequence.
ACCESSION   DBNGSX010000504 DBNGSX010000000
VERSION     DBNGSX010000504.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7471)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7471)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7471
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1782281"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="AC16CB_14925"
     CDS             complement(<1..1141)
                     /locus_tag="AC16CB_14925"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004591800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HIR3343813.1"
                     /translation="MNNPLEMGLEDPSGKIIRIAGPVVGAVGLNRVRLYDVVLVGELA
                     LVGEVIRLSGDLAIIQVYEDTSGVRIGEPVVNTGLPLVAQLGPGLLGSVYDGLQRPLK
                     VIAASTGDFIRRGVQAPPLPEEAVWTFTPHISKGDRVGPGDLLGVVPETKTLEHRILV
                     PPGRRGKVIETRSGETRLNDVVVKIESSENHHKELFEITLVQRWPVRQPRPIQTRLPP
                     KSPLISGTRVIDAFFPVAKGGSAIIPGGFGTGKTVVEHSLARWADADVVVYVGCGERG
                     NEMTEVLEEFPVLEDPRTGAPLMERTVLIANTSNMPVAAREASIYTGITIAEYYRDMG
                     YAVLLLADSTSRWGEALREISGRLEEMPGEEGYPAYLAARLAEFYE"
     gene            complement(1125..1472)
                     /locus_tag="AC16CB_14930"
     CDS             complement(1125..1472)
                     /locus_tag="AC16CB_14930"
                     /inference="COORDINATES: protein motif:HMM:NF014092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HIR3343814.1"
                     /translation="MSRLLVITRPHLVDGFRLAGVEAFAAEDVETAQELIESWVEAGE
                     SGLVAIDDAYLEHINPMVIRRLEASDQLDYLAIPGGEPLGPETSRRQRLAQQIRRAIG
                     FHITFKGEDEQSS"
     gene            complement(1469..2545)
                     /locus_tag="AC16CB_14935"
     CDS             complement(1469..2545)
                     /locus_tag="AC16CB_14935"
                     /inference="COORDINATES: protein motif:HMM:NF014094.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HIR3343815.1"
                     /translation="MADFDYGNARLRAMKSRLLAQRELETLAELGSMHGLISALAKTA
                     YQRSIETALTHASGLECIAQAIRMDLINTFGKARGFFANDAENMVAIVLRPYDLHNLK
                     AILRGLSRNIPSAEILNVLLPVGDLHYAVLAELTRVPGPRVAIDLLASMNEPIVQPLL
                     KLRARHPGADLPEMELALDQWHYQEAWIFVQATPSAHEVLASALKIEADTNNLLTVLR
                     FAHEPEERRHLLESGGAGGVIELLVEPGFLPRPLLVNLGNQDNLEAAIDLLAGTRYKP
                     ALRMGLQAFKKTGRLSEIEKHLKRYRLNWLKNLIFKDALGIGVFLGYVALKINEINNL
                     RWIAHGIHFGLEPREIKAELEVAG"
     gene            complement(2503..3144)
                     /locus_tag="AC16CB_14940"
     CDS             complement(2503..3144)
                     /locus_tag="AC16CB_14940"
                     /inference="COORDINATES: protein motif:HMM:NF014093.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HIR3343816.1"
                     /translation="MSLESILAAIRAGGDAQFREIESGARTQIYEIMAIARMEAEDIE
                     EKACNDAVAPSAKERARIIHRARLESMHIVGETCEGLINTTLERTRGVLAGLRTDRAY
                     PDVLCRLLEEALQELRSSLGDEGLIHLEADPRDADLLQSMLRQMGLELDVSFTLDSWG
                     GLIAKSEDGRVVVANILETRLERALPYLRQYLAAYFEDEQWQTSTTVTHAYAP"
     gene            complement(3146..3361)
                     /locus_tag="AC16CB_14945"
     CDS             complement(3146..3361)
                     /locus_tag="AC16CB_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HIR3343817.1"
                     /translation="MEGLTAIGAGLAIGLAAIGTGLAQARIGSAGAGVIAEKPEALGP
                     IILLIAIPETMVILGFAVAAMVLLLGG"
     gene            complement(3407..5287)
                     /locus_tag="AC16CB_14950"
     CDS             complement(3407..5287)
                     /locus_tag="AC16CB_14950"
                     /inference="COORDINATES: protein motif:HMM:NF013647.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HIR3343818.1"
                     /translation="MNIEMIPLQIIGLKSDLQDVLHSLRKIGCMHIDELTESPEVSAR
                     PLTLDREAIRKQEEMGFLLARIDGLLDVLGIHREPAEQSELSDARPGVEALMAKVQSL
                     TAQREKLQAEMASLPRYEATLKKLLPIIPPSARDPGQGTVGILVSRSHVGVLDSVGKT
                     AMDITNGRAEVVASDIDISTRAMLIVFPADFTAEIESLLGREDVSRLRLPAELGEGSP
                     DVVMAALRRRLLAIPEELKSIEQELAGLAVQWRNRLLSWREALREELDSTSILSRFGE
                     TDMTFVLVGWVPVVDLEKTREALRNIVDERIFIQRLPLTPELKKRAPVSLSNLLPAKP
                     FESLVNLLALPRYGHIDPTLLMAIFLPIFFGMILGDVGYGVLLLILCLGLIRRFKAGV
                     ARDVLTILAIGAGWSVLFGFLYGEAFGTLGEHWGMHALWFDRASPENVAGLLVMTIAI
                     GAAHITLGLVLGVWEAYRDRSRSHLLERSGMLVGLIGLFFLVGVLVDFLPQALMTPAI
                     VGIIIGIVLVGTPLGWLGILMGPIEFISLIGNVLSYLRIAAIGLASVYLAKVANDMAG
                     MVGNLIVGVILAILIHALNLVMGAFSPTIHSLRLHYVEFFRKFYEGGGRPYQPFRSQV
                     AK"
     gene            complement(5284..5631)
                     /locus_tag="AC16CB_14955"
     CDS             complement(5284..5631)
                     /locus_tag="AC16CB_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343819.1"
                     /translation="MVQDVNLSPLDQIRLCEAEVTRRIASARQAAEKSVLKARSESAT
                     LKQQSRERGIKRGQSQYEEIITRSEEEAKVMVAQAHRRSDELLRRGRRRIEEAVRQAV
                     NIVIGLDGEMSDP"
     gene            complement(6153..6839)
                     /locus_tag="AC16CB_14960"
     CDS             complement(6153..6839)
                     /locus_tag="AC16CB_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3343820.1"
                     /translation="MKALIVDDDRVLADVVAFALRREGFQVIQAYDGQTALACWAEDK
                     PDLVILDVNLPKMDGFTVCQRIRKEADTPIIMLTVRGEDGDVVHGLDLGADDYITKPF
                     SPGQLVARARAVLRRSGMPVIPSPRQVGDLALDPSRREVRIGRGQPVPLTHLEGRLLD
                     CLIVNVGQILTYDAIIDHVWGPRGGDRDMLRQLIRRLRGKIDSGLPSSVYIKTVAGLG
                     YGLIVEDGLS"
     gene            complement(6836..>7471)
                     /locus_tag="AC16CB_14965"
     CDS             complement(6836..>7471)
                     /locus_tag="AC16CB_14965"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3343821.1"
                     /translation="ALNASIELLLDQEKRGDPAERHALLKGLQVSVINLQTLVDNLLE
                     SDSFEAGHFRVWPRPYDLSDLVSEAVRTMQPLLEKYGQHLLIRLPDDIPLVHIDPRRT
                     AQVLVNLLSNASKYGPPDVEIILSVHVENNSVRVKVADSGPGISPKRRERLFQRFTYQ
                     DMEETNLKVGVGLGLSVVKAIIEAQGGRTGVDTPAGGGTIFWFTVPIVEEP"
BASE COUNT         1854 a         2208 c         1754 g         1655 t
ORIGIN      
        1 gctcgtagaa ttctgccaaa cgcgctgcca gatacgccgg atagccctcc tcgcccggca
       61 tttcctccaa tctcccagag atctcgcgta aagcctcgcc ccagcgagac gttgagtcag
      121 ccagtaagag cacagcatac cccatgtctc ggtaatactc agcaatcgta ataccggtat
      181 aaatgctggc ttcacgtgct gcaacgggca tattggaggt gttcgcaatg agcaccgtgc
      241 gctccatcag aggcgctcca gtgcgcgggt cttccaaaac agggaactcc tccaagacct
      301 cggtcatctc gttaccccgc tcgccgcagc ccacgtagac caccacatcg gcgtccgccc
      361 atcgagccag gctatgttcc accacagtct tacccgtgcc aaaaccccca gggataattg
      421 ccgaaccacc tttggctacc gggaaaaagg catcgataac acgcgtgccg ctgatgagtg
      481 gtgacttggg gggcaagcgg gtttgaatcg gacggggctg ccggacaggc cagcgctgga
      541 caagcgtgat ctcaaaaagc tctttatggt gattttcaga agattcaatc ttgaccacga
      601 catcgttcaa tctcgtttca ccgctgcgcg tctcaattac cttccctcgc cgacctggtg
      661 ggactaaaat gcgatgctct agggttttcg tttcaggcac gacccccaac aaatctccag
      721 gtccaacccg atcccctttc gatatgtgcg gtgtgaaggt ccatacagcc tcttctggca
      781 aaggcggagc ctgaaccccg cgccggatga aatcgcctgt ggaggctgca ataaccttca
      841 acggacgttg cagtccatcg tatacgctcc caagcagccc tggaccaagc tgtgctacca
      901 agggtagacc agtattgacc actggctcgc caatgcgcac acccgatgtg tcctcataaa
      961 cctggatgat cgccagatca cccgagaggc ggatcacctc gcccactagt gccaattccc
     1021 ccacaaggac gacatcatac aaacggactc ggtttaatcc cacagccccc accaccgggc
     1081 cggcaatgcg gataattttt ccggatggat cttccaatcc catttcaaga ggattgttca
     1141 tcttctccct taaacgtaat gtggaagccg atcgcccgtc gaatttgctg ggccaagcgc
     1201 tgccgccgcg aggtttccgg tccgagaggc tctccaccgg gtatggcgag atagtccagt
     1261 tgatcagaag cctccagacg cctgatcacc atgggattga tgtgctcgag ataggcatca
     1321 tcaattgcca ctaatccaga ttcacccgcc tccacccagc tctcaatgag ctcctgagcg
     1381 gtctctacat cctctgcggc aaaagcctca acccctgcca accggaagcc atcgaccagg
     1441 tgtggacgcg tgatgaccaa caaacgactc atccagcaac ctccagctca gcttttatct
     1501 cgcggggttc caacccaaag tgaatcccat gcgcgatcca gcgcaggttg ttaatttcat
     1561 taatcttgag tgccacataa cccaagaaga cgccgatgcc taacgcatct ttgaagatca
     1621 agttcttgag ccaattcaag cggtagcgct tcaggtgctt ctctatctcg ctcagacgac
     1681 cagttttttt gaaagcttgc aaccccatcc tcaatgcagg cttatagcgc gtccccgcta
     1741 gcaagtcgat ggctgcttcc aggttatcct ggttgcccaa attcacgagc agaggcctcg
     1801 gtaaaaagcc aggctcgacc agaagctcga tcactccccc agcacccccg ctctccaata
     1861 agtgtctacg ttcttctggc tcatgggcaa aacgcagcac ggtgagcagg ttatttgtat
     1921 ccgcttcgat ttttagcgct gaggccagca cctcgtgagc agagggcgtg gcttgaacaa
     1981 atatccaggc ttcctggtaa tgccattgat ccagtgccag ctccatctct ggaaggtccg
     2041 cacccggatg gcgagcgcgc aacttgagca ggggctggac aataggttcg ttcatgctgg
     2101 ctagcaagtc aatggctaca cgcggaccag gcaccctagt cagctcggcc aacacggcgt
     2161 aatgcaaatc gcccaccggc agcaatacgt tgaggatttc tgcggaagga atatttcggg
     2221 aaagaccacg taaaatcgcc ttgagattgt gcaggtcata aggtcgcaag acaatagcta
     2281 ccatattttc agcatcgttg gcaaagaaac cccgcgcttt cccaaacgtg ttgatcaaat
     2341 ccattcgaat cgcttgcgca atgcattcca ggcctgaggc atgtgtcagg gcagtttcaa
     2401 tcgatctctg gtaagctgtc ttcgccagcg cagagatcaa tccgtgcatg ctgcccaatt
     2461 ctgctagcgt ttcgagctcg cgctgtgcta gcagacggga cttcatggcg cgtaagcgtg
     2521 cgttaccgta gtcgaagtct gccactgctc atcctcaaag taagcagcca ggtattgacg
     2581 caaataggga agagcgcgct ccaagcgcgt ctctaaaatg ttggcgacca ccacacgtcc
     2641 atcttcactc ttggcaatca agcctcccca gctatccagg gtaaaactca catccagctc
     2701 caaccccatt tggcgcagca tactttgcaa aagatcagcg tcgcgaggat cagcttctaa
     2761 gtggatgaga ccttcatctc ccagagagct tcgaagctcc tgcaaagcct cttcaagcag
     2821 gcggcagagc acatcgggat aagctcgatc ggtgcgcaag ccagccagta cgcctcgcgt
     2881 ccgctcaagg gtcgtattga tcaacccctc gcaggtttca ccaactatgt gcatggattc
     2941 gagacgcgcc cggtggatga tgcgggcgcg ttcctttgca gatggcgcca ctgcatcgtt
     3001 gcaggctttc tcctcaatat cctcagcttc catgcgggca attgccatga tctcgtagat
     3061 ttgagtacga gcacctgact cgatctcgcg aaactgagca tcaccaccgg cgcgtatagc
     3121 tgccaggata gattcaagac tcatttcacc cgcccaagag caagaccata gccgccacag
     3181 caaagcccaa aattaccatc gtctccggga tggcgatgag caagataatt ggacccaggg
     3241 cttctggctt ctcggcgatg acacctgctc ccgccgatcc tatgcgcgct tgagctaacc
     3301 cagtgccaat agcagccagg ccaatggcca ggccagcacc aattgcagtt aatccttcca
     3361 tgtgaacctc cagttgaagg gatgtatacg acaacccttc gctaatttac ttggcaactt
     3421 gactgcggaa tggctgatat ggtctaccgc caccttcgta gaactttcgg aaaaattcaa
     3481 cataatgcag gcgtaaactg tggatagtgg gactaaaggc acccattacc aggttcagcg
     3541 catggatcag gattgccagg attacgccta caatcaagtt acccaccatc ccagccatat
     3601 cattggctac cttagccaga tagaccgaag caagcccgat agcggctatg cgcaaataag
     3661 acaggacgtt tccgatcagg ctgatgaact caatgggtcc catgaggata cccaaccacc
     3721 ccagtggtgt acccaccaat acaatgccaa tgatgatgcc tacaattgct ggtgtcatca
     3781 atgcctgtgg cagaaaatct accaagacac caaccaggaa gaataagccg atcagcccta
     3841 ccagcatccc actccgctcc aataaatggc tgcggctgcg gtcgcggtaa gcttcccaca
     3901 cacctaacac taatcctagg gtgatatgcg cagcgccaat agctattgtc atcactagca
     3961 aaccggcgac attctccggg ctggctcgat cgaaccagag tgcgtgcatc ccccaatgct
     4021 ctccaagcgt cccaaaagcc tccccataca aaaagccgaa caaaacagac caacctgcac
     4081 ctatcgctaa tatggtaagc acatcgcggg ccacgccggc tttgaaacga cgtatcaacc
     4141 caaggcagag aattagcagc aggacaccat aacccacatc ccccaagatc atgccaaaga
     4201 atatcggcaa aaatatggcc atcaatagcg ttgggtcgat atggccatag cgcggcaacg
     4261 ccagcaggtt taccaggctc tcaaacggct tggctggcag gaggttgctt agggaaaccg
     4321 gggcgcgctt ttttaattca ggagtcagcg gaagcctctg gatgaaaatc cgttcatcaa
     4381 caatattacg caaagcttca cgcgtttttt ccaggtcaac caccggaacc caccctacca
     4441 agacgaaggt catatctgtc tcaccgaaac gggagaggat actggtggaa tccagctcct
     4501 ctcggagtgc ctcgcgccaa gagagcaggc gatttcgcca ttgcaccgcc aatcctgcca
     4561 attcctgttc tatcgatttg agctcttcag ggatagccag taagcgacgg cgtaacgcag
     4621 ccatgacgac atccggagag ccttcaccta gctcagcagg taagcgcagc cgcgaaacat
     4681 cctcacgccc cagcagactc tcgatctcgg cggtaaaatc cgctgggaag acgatcaaca
     4741 tcgcacgcgt ggatatatcg atgtcgctag ctacgacctc ggcgcgccca ttggtgatat
     4801 ccattgcggt cttgccaacc gagtccaaca cacccacatg cgatcggctg accaaaatgc
     4861 ctactgtccc ctggcctgga tcgcgcgccg aaggtgggat gatggggagc agcttcttaa
     4921 gcgttgcttc atagcggggc aatgaagcca tctcagcctg cagcttctcg cgttgggcag
     4981 ttaaagactg gactttggcc atcagtgcct ctacccctgg gcgtgcatca cttagctcgc
     5041 tttgttctgc tggctctcga tggataccca gcacatccag taaaccatca atgcgggcta
     5101 acaggaatcc catttcctcc tgctttcgta tcgcttcccg atcaagcgtc agaggacgtg
     5161 cagatacttc cggtgactct gttagctcgt cgatgtgcat gcagccaatc ttacgcaggc
     5221 tatgtagcac atcttgcagg tcactcttta gaccaataat ttgcagcgga atcatctcga
     5281 tattcatggg tcggacattt ctccatccaa accgatgaca atatttaccg cttgacgtac
     5341 ggcctcctca atccgccggc gaccgcgacg gagtaattca tcagatcgcc tgtgtgcctg
     5401 ggctaccatt actttcgcct cctcttcgga gcgagtgatg atctcttcat attgcgactg
     5461 gcccctcttt atgccgcgct ccctggattg ctgtttcaat gtggccgatt ctgaacgtgc
     5521 tttcaacaca cttttttcag ctgcttggcg cgcagaagca atccgccggg taacctctgc
     5581 ttcacaaagg cgaatctgat ctaagggaga aagattgacg tcttgcacca tcactcctcc
     5641 aatatcgcat gtatttggaa caaaaggcat attgtgtgcc cccaccgcta tgtagcggcg
     5701 ggagtgtcag ttacactgcc attttgcacc aaagaccggt taaacacgat aaaatattgc
     5761 ggctaacgca ctgccccctc tcgccatatc aaagcaagat caatgttgtt acgcgctccg
     5821 cccccagata ctgcttccaa taaacggaag ccttttttcg catctgcttc gtttgggact
     5881 tctggcttga acaatagaag tccgtcctaa tttcaatgta tccggtgaga atgacaaaat
     5941 ctgtgacaaa gctgtgataa atttgtgaca aaattcactt aatgtcaaat atagaggccg
     6001 tttgtgctgt tagcgacccc gcaaattagc gaattaaaac aaaacgggtg cttttcagtc
     6061 acggatcgcc tgcaaaacac ccgatttggt atgaccctta gctaattttg ataactaaat
     6121 taactttttg gagcatgtat cctgagggag actcaactca acccatcttc cacgatcaac
     6181 ccatatccaa gtcctgcaac ggtcttgata taaactgaag agggcaagcc agaatcgatt
     6241 ttgcctctta agcggcggat caactggcgt agcatatccc gatcaccccc gcgcggcccc
     6301 cagacatgat caatgatcgc atcgtaagtc aatatttgcc ctacatttac tatcaagcag
     6361 tcgagtaagc gcccctcgag atgagtcaaa ggaacgggtt gcccccgacc aatccgcact
     6421 tcgcggcggc tgggatctaa tgccaagtcg cccacctggc gtggtgaagg gataacgggc
     6481 attccgctgc ggcgcagcac ggcacgcgct ctggcaacca gctgccctgg actgaagggt
     6541 ttcgtgatgt aatcgtccgc gcccaaatcc agaccgtgca caacgtcgcc atcctcgcct
     6601 ctcaccgtga gcatgatgat aggcgtgtcg gcttctttac ggatgcgctg acacacagta
     6661 aacccatcca tcttgggcag attaacatcc agaataacca gatcgggctt atcttccgcc
     6721 caacaagcca gggcggtttg tccgtcatag gcttggatta cctgaaatcc ttctctccgc
     6781 aaggcgaaag cgacaacatc cgccagcaca cgatcatcat caacaattaa tgctttcatg
     6841 gctcttccac tataggtaca gtaaaccaga atatagtccc gcccccagca ggtgtatcga
     6901 cacctgttct ccccccttgg gcttcaataa tagccttgac cacagacaag ccgaggccaa
     6961 cgccaacttt aagattagtc tcttccatat cttggtaagt gaatcgctga aaaagtcttt
     7021 cccgtctttt tggagaaatc ccagggccgc tatctgctac tttcacccgc accgagttat
     7081 tttccacatg cacgctcaat ataatctcga cgtcaggtgg gccatattta ctggcattgg
     7141 aaagcaagtt caccaacacc tgcgcggtcc gccgtggatc aatatggacc aatgggatat
     7201 cgtctggcaa ccttatcaga aggtgctgac catatttttc caacaggggc tgcatggtac
     7261 gcacggcttc agaaaccaaa tcagagagat cataaggacg tggccataca cgaaaatgcc
     7321 ctgcctcaaa actgtcactt tccagaaggt tatccaccaa cgtttgaaga ttaatcacgc
     7381 tcacctgcaa gcctttgagc agcgcatgcc gctcggcggg gtctcctcgc ttctcctgat
     7441 ccaggagcag ctcaatagaa gcattgagcg c
//
LOCUS       DBNGSX010000505         7139 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_605412, whole genome shotgun sequence.
ACCESSION   DBNGSX010000505 DBNGSX010000000
VERSION     DBNGSX010000505.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7139)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7139)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7139
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_605412"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="AC16CB_14970"
     CDS             <1..337
                     /locus_tag="AC16CB_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HIR3343822.1"
                     /translation="FILATGGILGGGIHFEYGQKPSESVFNLPVMAPDDRNNWFESQF
                     LTQLSHPIYQSGMLVSDEFKPIQANGSHLFENLYIAGNQIGSCDSIRERSAEGIALAT
                     AYSISNQLS"
     gene            347..1618
                     /locus_tag="AC16CB_14975"
     CDS             347..1618
                     /locus_tag="AC16CB_14975"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HIR3343823.1"
                     /translation="MQPIHHPVELSLDQCIKCNICTTVCPVSAVTDLFPGPKYEAPQA
                     GRFRAPGQQTPDHSVDYCSGCRVCNMACPTGVKIAEMNARARATLVEQGKISPILRLR
                     NNLIARAELLGKIANPIAPLANAILSNRAIRWTMEQALGIHREAPLPRFSGERFTTWF
                     NAHQSTNRAWRKVVYFHGCSTQYYEPRVGRAAIGVLEANGYEVIVPPQNCCGLPLLSN
                     GEFPAARNYHTSNVNHLVGYAKQGIPIVGTSTSCTLTLKEEAPELLDMVDEDTRLVAQ
                     STYDINEFLLDRFADGTLRIDFQPVPLMLAYHAPCQYRAHRLGKLALEILALVPELNI
                     LDSQVACCGIAGTYGYKREKFDVAMQVGEPLFNFVRDVGGPAVICDSETCRWQITQAT
                     GLPAIHPIEILAAAYGLKVEGILQDLLVSSY"
     gene            1615..2775
                     /locus_tag="AC16CB_14980"
     CDS             1615..2775
                     /locus_tag="AC16CB_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343824.1"
                     /translation="MTTKQLIHKVELLLILFITACTRSASTQGIPGFPALVSPTASQA
                     VSPASALQVPKSVLPTNTPIELSVPSTTETSQSQEIAVIPLPLDGPIADPLAEISGMA
                     WYADDLLLLPQYPDRFGDDGNGYIFGLNKKDILSYIQGATGNPLTPYLIPLITNNLEA
                     MLNGFEGFEAIAIEGDQIYLTVETKPAGMMGYLVSGKISPDLSQIILDTSRLISITPQ
                     TELTNYSDEALLVFGNRLFSIYEVNGAALNPTPLAHQFDFSLQVLDPLAFPNIEYRIT
                     DATSVNEFGRFWGINYFFPGESALKPDADPLERQYGEGPSHSRFETVERLVEFQFSYT
                     GIILSDTPPIQLELIDDNHSRNWEAIARLEGFGFLLSTDKFPETILGYIPYP"
     gene            2801..3751
                     /locus_tag="AC16CB_14985"
     CDS             2801..3751
                     /locus_tag="AC16CB_14985"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF002320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIR3343825.1"
                     /translation="MSGHPLRIFSGSSHPELASEIATQLGVPLGRSTTIRLPDSETHI
                     MIDEVVRDQDIFFIQTCPAPVNDHIMELLLYLDAFRRASAHDISVVIPYFPYARQDRM
                     ARGREAISARVVASLIESMGASRVIFVDIHNRAIQGFFNIPVDPLSALPMLANYFRKP
                     EFNDAAIVSPDVGRTTMAGHYAELLNLPLVVMHKRRTSISHTETTHVVGDISGRRPII
                     IDDLLAGGSVLRQIDALYDRGATGKAYFSITHPILLPTALQILDEDERIEKLIVTNTL
                     PVPPEKRHPKLEIISIAPLLADIISHIHQGLSISGKLVLE"
     gene            3762..5057
                     /locus_tag="AC16CB_14990"
     CDS             3762..5057
                     /locus_tag="AC16CB_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3343826.1"
                     /translation="MSKPSNYRWFVVGVFFLFMLLHQADKLLIGPLTTPIMRDFGIDE
                     VQMGAVFTGALLVGAFLYPIWGYLYDRFARAKLLALASFIWGSTTWISAIAPTFPTFL
                     ASRASTGIDDSSYPGMYSLISDYFGPAVRGKVYGLIQIAQPLGYMLGLLVATYLGVTL
                     GWRGVYYITGTLGIVLAVVILFGVKEMPRGKSEPELAEFEQISTYKFDIRIAKGLFRK
                     RSLLLLFAQGFVGVFPWNMITYWFFRYLETERNYSSQAVFMTMAPAILVLASGYFVGG
                     TIGDFFFKRTPRGRMLTAMFGVIIGAILFFLTMNVPIESQSLFMVMLMITAVFIPLSS
                     PNVISTVYDITLPEVRSTALAIQYFIENGGAALAPLLGGIIAKYASLHVAILLICVSA
                     WIIGAVLLGFTAYLVPKDIDTLRGQMRERADFERQVSAT"
     gene            5005..6516
                     /locus_tag="AC16CB_14995"
     CDS             5005..6516
                     /locus_tag="AC16CB_14995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3343827.1"
                     /translation="MVKCASVLISNGKSLLPDIILDMEIDQDKSKKTVAPLQETSARL
                     NIDRKWGVLIAVGAGTYMSALDISVVNTVLPIVNKTFNSDIATVEWVVIIYLLLVSGM
                     LPGFGRLGDLRGHKPVYIGGFILFIASSLLCAISPTILALIGARAFQSIGASMLSANS
                     PAILTKTFPANQRGRALGLMATMTYLGLITGPSLGGWLAEYYGWRSVFYINLPVGLIA
                     LLLSIWFIQKDAPEKITERFDWAGALTFLGGLVSLLLALNRGHDWGWSSPATLGLFAF
                     AIVLLGIFLWIESRVRAPMLDLSLFRIRSFSTSISSAVLNYVCVYSIVFLMPFYLIQG
                     RGLSPSQAGLLLTAQPIVMAIVAPISGTLSDTIGARIPGVTGMGILALGLFLLSRLNI
                     ASPPSMVALSLAVCGLGIGTFISPNNSSLMGSAPRHRQGIAAGMLATARNVGMVLGVG
                     LAGAIFTTVLTHGETGDQTVLYTAVQLSYSVIIFVALGGMLTSAIRGDDRNNI"
     gene            6583..6897
                     /locus_tag="AC16CB_15000"
     CDS             6583..6897
                     /locus_tag="AC16CB_15000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012255937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3343828.1"
                     /translation="MPKLPGTFQQFKEEHPAVWQAYENLGVAIAHSGPLQQKVRELIK
                     LGMAAAHQSESAVHSHTHRALEAGATQEEIEHAILQGITTIGFPKTMAALTWSKEARK
                     SR"
BASE COUNT         1736 a         1875 c         1656 g         1872 t
ORIGIN      
        1 cttcatcctt gctaccggag gtatcttggg tgggggaatc cattttgaat atgggcaaaa
       61 acccagtgaa agtgtgttca atttgcccgt tatggcacca gacgatcgca ataattggtt
      121 tgaatcccaa ttccttaccc aattaagcca tccaatttac caatcaggca tgttggtgag
      181 cgatgaattc aaacccattc aagccaatgg cagccatctc tttgaaaatc tctacatcgc
      241 tggcaaccaa attggttcct gtgattccat acgcgagcgt tccgccgaag gcatcgcctt
      301 agccactgca tacagcatat ccaaccaact gagctgacag ccaaccatgc aaccaataca
      361 tcaccctgtc gaactaagtc tagatcagtg tatcaagtgc aatatctgca caacggtatg
      421 cccggtttct gcggtaactg acttgtttcc tggcccaaaa tatgaagccc cccaggcagg
      481 gcgtttccgc gcaccgggac agcaaacccc tgatcattcg gtagattact gcagcggctg
      541 tcgtgtatgt aacatggctt gtccgacagg tgtcaaaata gccgagatga acgctcgagc
      601 ccgcgccaca ctggtggaac agggaaaaat ttctccgata ctgcgcctgc gaaataatct
      661 tatcgcccgc gccgagctac taggcaaaat agccaaccca attgcaccac tggcgaatgc
      721 cattttatca aatcgtgcca tccgctggac aatggaacag gcattgggta tccaccggga
      781 agccccccta ccccgtttct ccggagagcg atttactacc tggttcaatg cacaccaatc
      841 cactaacaga gcgtggcgaa aagtcgtcta ctttcatggt tgctccacgc aatattacga
      901 gccgcgcgtc ggacgtgcag caatcggggt gctggaagca aatggttacg aggtcatcgt
      961 gcctccccaa aattgctgcg gtctaccatt actttctaat ggcgagtttc ccgctgcccg
     1021 taattatcat accagcaatg taaatcatct agtaggttat gcgaaacagg gcattccgat
     1081 tgtcggcaca tccacaagct gcaccttaac cttgaaagaa gaagcacccg aattgctcga
     1141 catggttgac gaagatactc gcctggtcgc acaatccaca tatgatatca acgaattcct
     1201 gctggatcgc ttcgctgatg gcactctgcg cattgacttc cagcccgttc cactgatgct
     1261 agcctaccac gctccatgcc agtaccgcgc tcatcgtctt ggtaagctcg ctctggaaat
     1321 attagctctc gtcccagagc taaatattct ggatagccag gttgcctgct gcggcatcgc
     1381 cgggacctat ggctacaaga gagagaagtt tgacgtggcg atgcaggtag gagaaccact
     1441 ttttaatttc gtcagagacg ttgggggacc agccgtcatc tgtgatagcg aaacctgccg
     1501 ctggcagatc actcaagcta caggcttgcc ggcaatacat ccgatagaaa tactcgcagc
     1561 cgcatacggt ctaaaagtcg aaggcatatt acaagattta ctggtctcaa gctattgacc
     1621 actaaacagc ttattcacaa agtagagcta ctgctcatat tatttatcac agcttgtact
     1681 cgctcagctt cgacacaagg tattcctgga ttcccagccc tcgtctctcc aaccgcaagc
     1741 caggcagtat ctccggccag tgcgttacag gtgcccaagt ctgtcctccc caccaataca
     1801 cccatagaat tatccgtacc ttcgacgacc gaaacttccc aatcgcaaga aatcgcggtg
     1861 atacctctac cgcttgacgg cccaatcgca gaccctctgg ctgaaatctc cggaatggcg
     1921 tggtatgcag acgatttact tctgctcccc caatacccag atcgctttgg tgatgatgga
     1981 aatggttata ttttcggttt gaataaaaaa gatatcctca gctatatcca aggggcaacg
     2041 ggtaatcctt taaccccgta ccttatcccg ctcattacga ataatcttga agccatgctg
     2101 aacggctttg aaggctttga agccatcgcc atcgaaggag accagattta cttgactgtc
     2161 gagacaaaac ctgctggaat gatgggatat ctggtttccg ggaaaatatc ccccgatttg
     2221 agccaaatta tcctggatac atctcgcttg atctccatca caccgcaaac tgaattaact
     2281 aattacagcg atgaagcctt gctggttttt ggaaaccggc ttttcagcat ctacgaggtc
     2341 aacggcgctg ctctcaaccc taccccgctt gcccatcagt tcgacttctc attgcaagta
     2401 ctggatccat tggctttccc aaatattgaa tatcgaatta cggatgctac atcagtcaat
     2461 gaatttggcc gattttgggg gataaactat ttctttcctg gtgaaagtgc cctgaaacct
     2521 gatgcggacc cacttgagcg ccaatatggc gaaggtccct cgcatagccg ttttgaaact
     2581 gttgagcgtc tggtcgaatt ccagttcagc tatacgggca ttatcctatc ggatactcca
     2641 ccaatccaac tggagctcat cgatgacaac cacagtcgca actgggaggc gattgcccgc
     2701 ctcgaaggtt ttggcttttt actctctact gacaaatttc cggaaaccat cctgggatat
     2761 atcccctacc cataaccggg tataatctgt tgtatttcct atgtcaggac acccactacg
     2821 catctttagc ggatcgtcac atcccgagct ggccagcgag atcgccacgc aactcggcgt
     2881 cccgctcggc aggtcaacca cgattcgcct ccccgatagt gaaacccata tcatgatcga
     2941 tgaggtagtg agggatcaag atatcttctt tatccaaacc tgcccagcgc cagtcaacga
     3001 tcacattatg gagctgcttc tctatctcga cgcatttcgt cgcgccagcg cccatgacat
     3061 tagcgtggtc attccatact tcccttatgc ccgccaggat cgcatggcgc gcgggagaga
     3121 agctatcagc gcgcgcgtcg ttgcctcgtt gattgagagc atgggagcca gccgggtaat
     3181 ctttgtagac atccacaacc gtgctattca aggatttttt aatatccccg tagatcctct
     3241 gagcgcgctg cctatgttag caaactattt ccgcaagccg gagtttaatg acgcggcgat
     3301 tgtcagccca gatgttgggc gcacgacgat ggcaggccat tatgcagagc tgctcaactt
     3361 gcccctggtg gtaatgcaca agcgcaggac tagcatcagc catactgaaa ccacccacgt
     3421 ggtgggggac attagtggcc gtcgacctat catcatagat gacttgctcg ctgggggcag
     3481 cgtgttgcgc cagatcgacg ctctctacga ccgtggcgct accgggaaag cgtatttctc
     3541 tatcacccac cccatcttgc ttccaaccgc gcttcaaatc ctggatgagg atgagcgcat
     3601 tgaaaaactg attgtgacca acaccttgcc tgtgccacca gaaaagcgcc atccaaagct
     3661 cgaaatcata tctattgccc ctttgcttgc tgatatcatc agccacattc accaaggatt
     3721 gagtatctca gggaaattag tcctggaata ggagaagtaa gatgagcaaa ccttccaatt
     3781 atagatggtt tgttgttggg gtatttttct tattcatgct gctccatcaa gctgataagt
     3841 tgttgatcgg ccctttaacc acgccaatca tgagagattt tggaattgat gaagtgcaaa
     3901 tgggagctgt ttttaccgga gcactgctgg ttggggcttt tttatatccc atatggggat
     3961 atttatatga ccgcttcgct cgcgccaagc tgttagccct ggcatcattt atttggggat
     4021 caactacctg gataagtgca attgccccca ctttccccac ctttctcgcc agccgcgctt
     4081 caactggcat agatgactca agctatccgg gtatgtacag cctgatctca gattacttcg
     4141 ggcccgctgt gcgcggtaaa gtctacggct tgatccaaat cgcacaacca ctgggatata
     4201 tgctggggtt actggtagca acctacctgg gtgtaacttt gggctggcga ggtgtgtatt
     4261 acatcacggg cacgctggga attgtcctag ccgttgttat cttgtttggt gtgaaagaaa
     4321 tgccgcgtgg caaaagcgag ccggagctag cagaatttga gcagatcagc acatataaat
     4381 ttgatattcg gatcgccaaa ggattattcc gcaagcgcag tttgttgctc cttttcgccc
     4441 aaggatttgt aggcgttttc ccttggaaca tgattaccta ctggtttttc cgttatcttg
     4501 aaaccgagcg caattactct tctcaggcag tatttatgac catggccccc gccattttag
     4561 tgctggcatc tggttacttc gtaggaggca ccatcggtga ttttttcttt aaacgcaccc
     4621 cccgcggacg gatgctcacg gctatgtttg gcgttatcat aggcgccata ttatttttcc
     4681 taacgatgaa cgtcccaatc gagagccagt ctctcttcat ggtcatgttg atgataaccg
     4741 ccgttttcat tcccctgtca tcacccaatg taatctccac agtctatgac atcacgcttc
     4801 ctgaagtgcg cagtacagcc cttgcaattc agtattttat cgagaatgga ggcgctgcat
     4861 tagctcccct attaggaggc attattgcca agtatgcctc gctccatgta gccatattgc
     4921 tcatttgcgt gtccgcgtgg atcattgggg cggtcctgtt gggatttact gcctacttgg
     4981 tacctaagga tatcgacaca ctgcgtggtc aaatgcgcga gcgtgctgat ttcgaacggc
     5041 aagtctctgc tacctgatat cattctggat atggaaattg atcaagacaa aagcaagaaa
     5101 actgtagcac cgctacaaga gacatctgcg cgactcaaca tcgatcgcaa gtggggagtt
     5161 ttgattgctg tcggtgcggg tacttacatg tcggcactgg acataagcgt cgtcaacact
     5221 gtgctcccta ttgtaaataa aaccttcaat agcgacatcg ccaccgttga gtgggtagtg
     5281 atcatctatt tattgcttgt cagcggtatg ctgcccgggt ttggccgctt aggtgacctg
     5341 cgcggacaca agcccgtcta tatcgggggc tttatactct ttattgccag ctctttatta
     5401 tgtgctatct cgcccacgat attagctctc atcggagcac gcgcatttca atccattggc
     5461 gcgtcgatgc tctccgctaa ttcccctgcc attctcacga agaccttccc cgccaaccaa
     5521 cgcggtcgcg ccctgggctt aatggcgacc atgacctact tgggattgat taccggtcca
     5581 tcattaggag gctggctagc agagtattat ggttggcgtt ccgtatttta tattaatttg
     5641 ccggtcggct tgatcgctct acttctcagt atttggttca tccaaaaaga tgcccccgaa
     5701 aaaatcacag aacgatttga ttgggcaggt gctctaacct tcttgggtgg gcttgtatcg
     5761 ttactccttg ccctaaatcg gggtcatgat tggggctgga gttcgccagc cacactgggg
     5821 ctgttcgcgt ttgctatagt tcttctgggt atctttctat ggatcgagag ccgagtccgt
     5881 gcaccaatgt tagatctcag tctcttccgc atccgctcat tctccacttc aatcagcagt
     5941 gcggtgctaa actatgtttg tgtatatagc attgtcttcc tgatgccatt ctacttaatc
     6001 caaggacgtg gtctgtctcc ttcacaagca ggtctattgc tcactgcaca accgattgtg
     6061 atggcaattg tcgcacctat tagcggaacc ttatctgaca ccattggagc gcgcatccca
     6121 ggggttacag ggatgggaat tttggcattg gggctatttt tactttccag gttgaatatc
     6181 gcctctccac catcaatggt agctctatct cttgcagtgt gcgggctcgg tattggcaca
     6241 ttcatttcac ccaataatag ctccttaatg ggatcagccc cacgtcatcg tcaaggaatt
     6301 gctgcaggca tgctagcgac agccaggaat gtgggtatgg ttttgggagt aggcttggca
     6361 ggagcaattt tcactaccgt cctaacccat ggagaaaccg gagatcaaac cgtcctttac
     6421 actgcagttc aattgagtta ttctgtgatt atctttgtcg cgctgggtgg catgctaact
     6481 tctgctatcc ggggtgatga tcgcaataac atttaaaatc aaccccacca cccaagacat
     6541 acattcatca ataatgggta aaaataaccc ataggaggaa ctatgccaaa actacctgga
     6601 acctttcagc aattcaaaga agagcaccca gcggtgtggc aggcttatga gaacttgggg
     6661 gttgccattg ctcattccgg cccgttgcag caaaaagtcc gtgaattaat caagctgggt
     6721 atggcagctg cccaccaatc tgagagcgcg gtacactcac acacccaccg cgcccttgag
     6781 gcaggtgcca cacaagaaga gatcgaacac gccattttgc aaggtattac taccatcgga
     6841 tttcccaaaa caatggcagc cctaacatgg tcaaaagaag ccagaaaatc acgctaagga
     6901 taacagcact cctcgagagt aacttgcatg gcatcggctt tttgatatgt attctccgcg
     6961 actgtaagtg agctaggacg tataaattat aggacttgcg cattggaaga aagattttgt
     7021 tctctttgag gagcaaacat ggacgaaaga ccaggaggtc tctctgggtt gatcaaactc
     7081 gccctgctgg gttggctatt cgattggctt caagataatt ttggctttgg gcgaggtgt
//
LOCUS       DBNGSX010000506        13458 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1505602, whole genome shotgun sequence.
ACCESSION   DBNGSX010000506 DBNGSX010000000
VERSION     DBNGSX010000506.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13458)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13458)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13458
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1505602"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..512)
                     /locus_tag="AC16CB_15005"
     CDS             complement(36..512)
                     /locus_tag="AC16CB_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343829.1"
                     /translation="MTKDDADSQVPFIWIELPSKLEIDRNDIRVFMARRDPPGMLVPT
                     ETELPCIIQSANEHVELRPVALPFMYDGHQLVGSYAWLACASCVECYMNWETTLEMVA
                     TLEEDMMTISFGVRHMGHNIQEDYLRIELEQASPTAKEPGIQCWSAENCKQMEFAR"
     gene            1958..2578
                     /locus_tag="AC16CB_15010"
     CDS             1958..2578
                     /locus_tag="AC16CB_15010"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003955703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3343830.1"
                     /translation="MDQQPSNIQPFDPQPVTLIGHTVRLEPLTQAHVPDLAIAGKDEE
                     IWRYMLYGPVINEKSMQLWVKDMLQRQARGTDLPFAVIHLGSGRAIGATRYMEIRPEH
                     RGLEIGGTWYALAHQRTAVNTESKYLLLQHAFETLSCIRVQFKTDLRNERSQRALERI
                     GAVWEGVLRNHMILGDGVYRHSVYYSILIEEWPSVKGKLEVLMTQK"
     gene            complement(2624..6907)
                     /gene="rpoC"
                     /locus_tag="AC16CB_15015"
     CDS             complement(2624..6907)
                     /gene="rpoC"
                     /locus_tag="AC16CB_15015"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR3343831.1"
                     /translation="METKGLTRLRISLASPETIQSWSYGEVLKPETINYRRLRPEKDG
                     LFCEAIFGPTRDWQCYCGKYKNIRYKGIICDKCGVEVTRSSVRRERMGHIELSAPVAH
                     VWYTRRIPSYLGLLLDISRRNLDRVLYFAQYVVTYVDEDARQKALKRLEDKITVTERE
                     QALKINTRIAEVKGERDRTLAELNQRRSEIEKRFEDTIAEQLDPIIKGGQRLEASLRE
                     QMGQAPRTAIHFPDTDIVIVEAGKTINNRNLSSVQSVVKERLEELENELKDQKQRELD
                     SNKMEVERVRAQADLEMENLRMQLDDQASSVRDENARMRDELLELRPLTFMGESRFRE
                     LKQRWGQVFRADMGAEAFYDILRRLDLDKLAQELWVEVRTSRSKQKRKKATKRLKVVE
                     AFRRSANRPEWMVLTILPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLL
                     ELGAPDVIVRNEKRMLQEAVDSLIDNSQRGKALSRRGRRELKSLSDMLKGKKGRFRRN
                     LLGKRVDYSGRSVIVVGPKLKLYQCGLPKNMALELYRPFVISRLVTHNYAANVKGAKR
                     FIERNRPEVWEVLEEVIRERPVLLNRAPTLHRLGIQAFEPVLIEGSAIQLHPLVTTAF
                     NADFDGDQMAVHVPLSQKAVWEARQLMLSSKNLLKPADGEPIISPSKDMVLGVYYLTK
                     SDESVHNGDGHIFGNMDEVELAYSLNIVDIHSRIKLRTDTWFDDQNNRLPKAEDRIIE
                     TTVGRVIFNRVLPTQMQYVNWVLDKGGLKDLIADLYEICGEENTPDIADAIKDIGFTY
                     ATRSGYSLAVSDITVPLEKQSIIQDALQEQETVQRDFRRGLLTEQEQNERIIEIWQRT
                     TNSVADAVKRTMDPNGNLAAQAISGATKGGFGPISQLAGMRGLMADPSGRIIPLPIRS
                     NFREGLTALEYFISTHGARKGLADTALRTADAGYLTRRLVDVAQDVIINDDDCGTADG
                     IWIRRSDDIAGQSISVRLYGRLVAERVVDPETGEILAEYNDVLDHERVRRIANSNVQE
                     VKARSPLTCELIHGICTKCYGLDLGRGKLVEVGSAVGIVAAQSIGEPGTQLTLRTFHT
                     GGVAAGGDITTGLPRVEELFEARKMPKGEAVVTEIAGRASVMQSDRYSDLRMVRVEHS
                     EMVSNEYSIPEEWTIVAEDGSEVSQGDVLAFLGEDASITTEHAGRVRLEDRNVIVSYE
                     DRQEKEYEIPSTARLIVRDGDLVEPGQPLTEGSLNPHQILRIQGRTACQIYLLSEVQK
                     VYRSQGQNINDKHFEVIIRKMMSKVQVLRPGDSGYLPGDLMDRLEIKRTNEQLLTDSK
                     RVAKSVDVLLGITKASLSTDSFLSASSFQHTIKVLAQAAIAASEDPLYGLKENVIIGK
                     LIPAGTGFVRGRFGDENLPQYVDSGEDVGDDETDDIFDAEFDAQTFAAD"
     gene            complement(7217..10540)
                     /locus_tag="AC16CB_15020"
     CDS             complement(7217..10540)
                     /locus_tag="AC16CB_15020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3343832.1"
                     /translation="MTFLLSLFTSPAYIDLPQSLVGRLIVGVFWVLGVALLVLFLRRW
                     RAFHRSRTTRFWGILAILAVFVPLTSLFIGLRLAARGALPPPGIPIDRLGPAMMVFIA
                     VPWTLAGGFLGIVPAAALAGFSGLLLGLWDTHNPFTPLEFAFLGILFSAAINQRYRTR
                     SYVLLRHPLFTSLLIAAFYPLIALVSASLIADGPLAIRLDYAIARLGAASLSVGIPLL
                     VAGLFSEVVAASFPGAWGRIGLLLPSPAEKSLQARFLYGTAPLAFILTVLLMVGDWVV
                     AGAAARQILRDRMADAARLASEGVPFFLEAGQNLIAQLSSDPRLYTSDPDDLIGVLAQ
                     DLRSVPFFTQLFILDEVGNPLAGYPIENYASAQPPPEEQMGIQLSLAGVPYQIYTVPP
                     DSDDKASKVSFVAAIRDENESVRGVLIGRTELDVNPFTKPIIASLSSLAGSDGEGILL
                     DEDGRILYHSNPALLMAVYTGRTAEGPEFYNDTAPDGTRRLVYYQPALGRPWAVVLTV
                     PARRTQQIALNIAGPLLGMIVLLAIFSVLLLRFGLRVVTASLKRLAVQAGVIAQGQLD
                     TPISFNGEDEVGQLGRAFEQMRLSLKARLDELNRLLLVSQGVASSLEMDEAVKPLLEA
                     SLSSGASSARVVLAPETVPDLEGSPVSPTVYAIGSAGETYAYLDDQILELTRNQERVV
                     MHSLARPRLINFKADDLRPQALMALPLRHENQYYGALWTAYDQSHKFGEEEIRFQVTL
                     AGQAALAAANARLYMNAEIGRQRLAAILASTPDPVLVTDQQNRLLLSNPAAWRALGLG
                     VEWEEGQPIERAVSEIPLLELIHSSDDKRSVEVTLPDGRVYYATASSVIADGIRVGRV
                     CILRDITYFKELDSLKSEFVSTVSHDLRSPLTLMRGYATMLEMVGELNEQQAGYVRKI
                     VTSVESMTKLVGNLLDLGRIEAGIDLQLEMIHVQDVIERVIGALQLQATQKRVQLVNE
                     VVRQVVPVIEADQALLQQALHNLVDNAIKYTESGGKVSVRVRTKVGVASEPDYLVIEV
                     QDNGIGIAPVDQPRLFEKFFRGAQEGGKKQGGSGLGLAIVKSIAERHGGKVWMESQLG
                     RGSTFYLSIPFRQRKGDLT"
     gene            complement(10650..11378)
                     /locus_tag="AC16CB_15025"
     CDS             complement(10650..11378)
                     /locus_tag="AC16CB_15025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3343833.1"
                     /translation="MSERSTSLYSSKYRHTDWDRRRRVLRWLLKYIGFTLLAKLDRVE
                     GLENVPAQGPAILMINHIAFIDPIVVLHVMPRNIIPLAKIEVYNYPLVGIFPRLWGVI
                     PVRREEVDRRAVQQVLEVLRAGEMVLVAPEGTRASHLSRGKEGIAYLSSHSGTPIVPV
                     AIEGTEGFPSLRFFSPAWKTDGAVVRFGRPFRFRPDLKRPDREQLRQMSDEAMYILAA
                     MLPERRRGYYADLSQATQETIEWV"
     gene            complement(11392..12159)
                     /locus_tag="AC16CB_15030"
     CDS             complement(11392..12159)
                     /locus_tag="AC16CB_15030"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HIR3343834.1"
                     /translation="MSTRRQLALFSSMLAIYAALTFISYYGFLDQMTQLPGVEPDFDY
                     NQRWIYGLANAGLIIVVYGLLGLAGFWFARKLNWPGIYREGADWRGWLLKPMAIGLLL
                     GFIVIVMDRIFAVLIAWDGFPHPQFPLSLIASATAGIGEEIIFRVFVMGLWAYLLNRF
                     LSRWISMRVAFWVANFIAAMAFGAAHLGSAMVILGGTSLDVISPLVMGEIFLINGIVG
                     MVAGERYVQQGLVAAAGIHFWMDIVWHVVWPVFLPSL"
     gene            complement(12163..12888)
                     /locus_tag="AC16CB_15035"
     CDS             complement(12163..12888)
                     /locus_tag="AC16CB_15035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3343835.1"
                     /translation="MPTQPGSHPDAKPRSTLIRPEITRLPSLTFWRRVFRRLIKWFVR
                     VLVWLFTEMRTEGLEHVPRHGPILVVSNHLGDADLIVGMAYSPYEVEVLSKAELYDYP
                     ILGILMDAYGIIWVHRGQPDRRALRAALEALRQGRVVGIAPEGRESLTGSLEEGTGGA
                     AYLALKANVPILPVTFTGTENKRIYANMKRLRKTRVTMTVGAKFRLENLPDRRKAIDA
                     GTQKIMQTLAEQLPLDYQGVYPK"
     gene            complement(12860..>13458)
                     /locus_tag="AC16CB_15040"
     CDS             complement(12860..>13458)
                     /locus_tag="AC16CB_15040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3343836.1"
                     /translation="VNIIGMENIPRQGGYLIAANHVSLYDPPLVLAFWPTEPEAVGAV
                     DIWQRPGQSLLVRMYGAIPVHRGEYDRHLMNTAQAALLSGKSLMIMPEGTRSHAPGMR
                     RAAPGVAYLIEKTDVPVVPVGVVGTTDDFFQRAMRGEKPSLYMYIGAPFRLPLVDGKG
                     EDRRRKRQENADLIMAHIAALLPPEYQGVYANTTRISS"
BASE COUNT         3406 a         3763 c         3212 g         3077 t
ORIGIN      
        1 tatcttaaag gataaagagg gaaacgtcaa gcaggttatc ttgcgaattc catttgcttg
       61 cagttttctg cgctccaaca ttggattccg ggctccttcg cagtggggct tgcctgctca
      121 agctcaatgc gaaggtaatc ttcctggata ttgtgcccca tgtgacgcac accaaatgag
      181 atggtcatca tgtcctcttc caaagtcgcc accatctcca gcgtagtctc ccagttcata
      241 tagcattcaa cgcatgacgc gcatgctagc cacgcatagc ttccgaccag ctgatgacca
      301 tcatacatga aaggcaatgc cacggggcgc agctcaacat gctcatttgc agattgaatt
      361 atgcatggta gctcagtctc cgtaggtact agcatgcctg gaggatccct acgagccatg
      421 aacacccgta tgtcattgcg atcgatctca agcttggagg gaagctcgat ccaaatgaac
      481 ggaacctggc tatcagcatc gtctttggtc atgtagactc tcgtatcagg tggaggagct
      541 tgatcgaata aagaagcatt actgcacgcg accagaccaa aaaacagcac ccccaaggta
      601 attatttctc tggcatccat tgaaggccat ttaatatgca caagcctaag cgagcttcag
      661 catagcgcgc ttccgagtca agaaccgggc aggcgtcatc ccttaaaact tgaaatctga
      721 acaatccata caataatatc ttccaccttt caccacatat gcatcttcgc ttagagagat
      781 aaccttccta cattttgtgc accgagatgg tttaaatttt ggtggattct ccaatttctc
      841 aaagttgtat tcgttcgtgc catagtagac atacatttca gtttcaatgt cttctcgaca
      901 ctgagaacat gttttccaat ctccagtatg attttctaca aaatgataac cacacaacgt
      961 gtatcggcga tggttacgga agcagctatt tcgcgcataa gaaaacataa catattgatc
     1021 ctcgtcatca catatccatt ggttgcaaca tttagttttg gttaagttct ttttcttgcc
     1081 gcacaagccg catcttggaa ttgatctaac atcttttgtg aggggtcttt ttccttgttt
     1141 tgacatttat ctctctcgga aagaaattta ttgggttaga ttcgggagca caaacattca
     1201 tggagaacaa cattcaaacg atgtgcgcgg aataagtatg taggcaactg cttaacagac
     1261 gtgaatacgc tcgatagttt cctatgtgca gaagtaagaa atattcttaa ttctactatg
     1321 gtatgttgga ttggctatgc tgaaatcccc tctaaaacca tagcaagcaa tccgatttct
     1381 acccagattg ctgctcccag agaaaatccc agcaatgcgg ttgcctcatc ttcacaccta
     1441 ggaattcgac ctccaacgac cagacccagc caggctacaa agaagcctta tacgatggag
     1501 catggcaggc cgacaactca acagagagat tacctgcatt tatcccataa actttcataa
     1561 aatcgtatga ctcaaggtag ccagcgggta tccacgtggg cttgacgtac agctggacaa
     1621 catcatgaag agtatggatg gaagaatgaa aagtgaagta atgctttcca ttgatcccat
     1681 cccttatgtg gcctaacgat aactcaattg gcagcacctt cagaactacc tacgccaatt
     1741 tatttattca cttttttgca gattagactg acacagctag gaccaggata taatttctgt
     1801 aaccattaat cacacctctg acgccagacc ctttgagttt attattgggt aatcatatca
     1861 aaaatcaagc gccaaatctg caccggcgca tcaaaatcca ggtttcttta taagtaatca
     1921 gcgcctgatg aaatgccccc acatgggtgt ctaagacatg gaccaacaac cctccaatat
     1981 tcaacccttc gatcctcaac cggtgaccct cattgggcac accgttcgcc tagaacctct
     2041 gacccaagct catgtccctg atttggccat agctgggaag gatgaagaaa tctggcgtta
     2101 tatgctctat ggaccggtca tcaatgagaa gagtatgcaa ttgtgggtga aagatatgct
     2161 gcagcgccag gcgaggggca ccgatctccc ctttgcggtg atacacctgg ggagcgggcg
     2221 tgctatcggg gcaactcgct atatggagat ccgcccggag catcgcggct tggagatcgg
     2281 tggcacgtgg tacgccttag ctcatcagcg caccgcggtc aacaccgaat ccaaatatct
     2341 attactccaa cacgcctttg agacgcttag ctgcatccgc gtacaattta agactgatct
     2401 gcgcaacgaa cgctcccaac gcgccctgga acgtattggc gccgtctggg aaggcgtgct
     2461 gcgcaaccac atgatcctgg gcgatggcgt ttaccgtcac tcggtatatt acagtatcct
     2521 cattgaagaa tggccttcgg taaagggtaa attagaggtt ctcatgacgc agaaataaaa
     2581 agtgggcggt ttctgaaccg cccacttttt atttttattt ttatcaatcc gctgcgaaag
     2641 tttgcgcatc aaactctgcg tcgaagatgt catccgtttc gtcgtcccca acatcttcgc
     2701 cgctatctac atattgcggc aggttctcat caccaaaacg gccgcgaaca aatcccgtcc
     2761 cagcagggat aagcttgccg ataatgacat tctctttcaa gccatatagc ggatcttcac
     2821 tggccgcgat agcagcctgt gccagcactt taattgtgtg ctggaaggat gaggctgaaa
     2881 ggaaggagtc cgtactcaga gatgccttgg tgatcccaag cagaacatcc accgatttgg
     2941 ctacacgctt gctatccgtg agcaattgct cgttggtacg cttgatctct aaacgatcca
     3001 ttaaatctcc aggcaagtag ccactgtcgc cggggcgaag cacctggacc ttagacatca
     3061 ttttacgaat aatcacttca aagtgcttat cgttgatgtt ttgaccctgc gacctgtaga
     3121 ctttctgtac ctcagatagc aggtatatct ggcacgccgt gcgtccctgg atgcgcagaa
     3181 tctgatgcgg gtttagggag ccttcagtca gcggctggcc tggctcaacc agatcgccat
     3241 cgcgcacaat caggcgggca gtgctgggta tttcgtactc cttctcttgc cgatcctcat
     3301 acgagacgat cacattccgg tcttccaggc ggacgcgacc ggcatgctca gtagtgatcg
     3361 aggcgtcttc accaaggaat gcaagcacat caccttggct gacctccgat ccatcttcgg
     3421 caacgatggt ccattcctca gggatgctat actcattact taccatctca gagtgttcca
     3481 cccgcaccat gcgcaaatcg ctatatcggt ccgattgcat cacagaagcg cgaccagcga
     3541 tctcggtgac aaccgcttcg cccttgggca tcttgcgggc ttcgaagagc tcttcaacac
     3601 ggggtagacc cgtcgtaatg tcaccacccg ccgcgacgcc gccagtatgg aaagttcgca
     3661 gggtgagttg cgtgcccggc tcgccgatgg attgagcggc tacaatgccg accgccgaac
     3721 ccacctccac cagtttaccg cgtcccaaat caaggccgta gcacttcgta catatgccgt
     3781 gaatcaactc gcaagtcagc ggtgatcgag cttttacttc ctgcacattg gaattggcaa
     3841 tacgacgcac gcgctcatga tccagcacat cgttatactc ggccaatatc tcgccagtct
     3901 ctggatcgac tacgcgctct gccaccaatc ggccatatag ccggacagat atcgattgac
     3961 cggcaatatc atcactgcgc ctgatccaga ttccatccgc tgtgccacaa tcatcatcat
     4021 taatgatgac atcctgagcc acatccacaa gacgccgcgt gagataacct gcgtcagcgg
     4081 tgcgcagggc cgtgtcagca agacccttgc gtgcaccgtg ggtagaaata aagtattcca
     4141 gcgcggtcaa gccttcgcgg aagttcgaac ggatgggcag ggggatgata cgtccggaag
     4201 gatcagccat caggccgcgc ataccagcta actgggaaat aggcccaaag ccacctttgg
     4261 ttgctccaga gatagcctgc gctgccaagt tgccgttagg atccattgtg cgctttacag
     4321 catcggcaac ggagttggtc gtgcgctgcc agatctcgat gatgcgctca ttttgctcct
     4381 gctcagtgag caaaccacgc cggaagtcgc gctgcacggt ttcttgctcc tggagcgcat
     4441 cctgaatgat gctctgtttc tccaacggca ccgtgatatc agacacggcc agggagtagc
     4501 ccgaccgggt agcataagta aagccgatat ctttgatggc atccgcgata tcaggtgtgt
     4561 tctcttcacc acagatttca tacaggtcag ctattagatc tttcaaccca cccttgtcca
     4621 gcacccagtt tacgtactgc atctgggtcg gtaggacacg gttgaagatc acgcgcccaa
     4681 ccgttgtctc gatgatgcga tcttccgctt tcggcaagcg gttgttttga tcatcaaacc
     4741 aggtatctgt cctaagcttg atacgagaat ggatatcgac tatattcaaa ctgtaggcca
     4801 gttctacttc gtccatgtta ccgaaaatat gcccatcccc attatgcacg ctttcatcac
     4861 tcttggtcag ataataaaca cccaagacca tatccttgga tgggctgatg atcggctcac
     4921 catcggccgg tttcagcagg ttcttggaag agagcattag ctggcgtgcc tcccagacag
     4981 ctttctggga gagcgggacg tgaaccgcca tttggtcacc gtcaaagtcg gcattgaaag
     5041 cagtggtaac caatgggtgc aactggattg cgctaccttc gatgagcacc ggctcaaacg
     5101 cctggatgcc caagcggtgc agcgtgggag cccggttaag taaaactggt cgttcccgga
     5161 tgacctcttc tagcacttcc caaacttcag gacggttgcg ctcgatgaag cgcttggcgc
     5221 cttttacatt ggcggcgtag ttatgcgtca ccaggcggga gattacaaag ggacggtaca
     5281 gctcaagggc catgttcttg ggcaagccgc attgatacag cttaagcttc ggaccaacca
     5341 caatcaccga acgccccgag tagtccacgc gcttacccaa aaggttgcgg cggaagcgac
     5401 cctttttgcc tttgagcata tcgctgagtg atttcaactc gcggcggcca cggcgagaga
     5461 gcgctttacc gcgctgcgaa ttatcgatga gcgagtcaac tgcctcttgc aacatgcgct
     5521 tttcgttgcg cacgatgaca tctggagcac ccaattccag caagcgcttg aggcggttat
     5581 tgcgattgat aacccttcgg tacaggtcgt tcaggtcaga ggtggcaaaa cgcccaccgt
     5641 ctaactgcac cataggacgt agatcaggcg ggataaccgg caaaatggtc aaaaccatcc
     5701 actccggtcg gttagcagaa cggcggaaag cctcaacaac cttaagacgt ttggtcgctt
     5761 ttttgcgctt ttgcttgctg cgcgatgtcc tcacttccac ccataattcc tgagccagct
     5821 tatccagatc caggcggcgc aagatgtcat agaaagcttc tgcacccatg tcggcgcgaa
     5881 aaacctgtcc ccagcgttgc ttaagctcac ggaagcggct ctcacccata aaggtgagcg
     5941 ggcgcaattc cagcagctcg tctcgcatgc gggcattttc atcgcgtact gaagatgcct
     6001 gatcgtccaa ttgcatgcgc aggttctcca tctcaagatc agcctgcgcg cgcacgcgct
     6061 cgacttccat cttgttgcta tcaagctcgc gctgcttctg atccttcagc tcattctcaa
     6121 gctcttccag gcgttccttg accacgctct gcacgctgga cagattccgg ttattgattg
     6181 tcttgccagc ttcaacgatg acgatatcgg tgtcggggaa atgtatggct gtgcgaggtg
     6241 cctgacccat ttgctcacgt agagaagctt caaggcgctg cccacctttg atgatagggt
     6301 cgagctgctc agcgatcgta tcttcaaatc gcttttcaat ctcggacctg cgctgattca
     6361 actctgccag agttcgatca cgctctccct tgacttctgc aatgcgggtg ttgattttta
     6421 acgcttgctc gcgctcagtg acagtaatct tgtcctccaa gcgcttgaga gctttctggc
     6481 gtgcatcttc atcaacgtag gtcaccacat attgagcgaa atacaacacc cggtctagat
     6541 tgcgccggga aatatccagc aacaaaccta aatacgacgg aatgcggcgt gtgtaccaaa
     6601 cgtgcgctac aggggcagat aactcgatat gccccatgcg ctcgcgacgc actgaagagc
     6661 gagtgacttc taccccgcat ttgtcacaga taataccttt ataacgaata ttcttatatt
     6721 taccgcaata gcactgccaa tcccgtgtgg gaccaaagat cgcctcacag aagaggccat
     6781 ctttctctgg gcgcaatcgg cggtagttga tggtttctgg ctttaagacc tctccataag
     6841 accaactctg gatagtctct ggcgaagcca ggctgatacg aagtctggtc aaccctttag
     6901 tttccactcg gctgcctcct acatctaatc tctaatccac cactaaccta aaaagaagac
     6961 acacttttac catccacagc ggtttcgctg ccgctgttga aaccgttttt gagtggcgtc
     7021 ttagcatcaa ttggttcggg gatcgtaata tttaaaagct taatacaaac aaagacaagc
     7081 gcattgagca cctccgtctc aagcgcttat cacgcgcgct acctcattga tttatcatct
     7141 gaatacatta taccaaccat gatcacggtt ggtcaagagg aaaattacgc aaatttcact
     7201 tgaatcaaaa aatcaattac gttaaatccc ctttccgctg tctgaatggg atggataaat
     7261 agaaggtact tcccctgcct aattggctct ccatccacac tttgccaccg tggcgttcag
     7321 caatcgactt aacgatggct aaaccaagac cagatcctcc ttgtttcttt ccaccctctt
     7381 gcgcgccccg aaagaacttc tcgaataatc gtggctgatc cactggtgct atgccaatgc
     7441 cgttatcctg cacctcaatc accaggtaat ctggttcgct ggcgaccccc actttggtcc
     7501 gcacgcgcac actcaccttc ccaccgctct ccgtatattt aatggcgtta tccaccagat
     7561 tgtgcaaagc ttgctgcagc aatgcctggt cagcctcaat aaccggaact acttgccgca
     7621 caacctcatt aaccagctgc acacgcttct gagtggcttg cagttgaagg gcgccaatga
     7681 cgcgttcgat cacatcctga acatggatca tctctaattg aagatcaatg cccgcttcta
     7741 tgcgtcccag gtcgagcagg ttgccaacca gcttggtcat cgactccacg ctagttacaa
     7801 ttttacgcac ataaccagcc tgttgttcgt ttagctcacc taccatctcc aacatggtgg
     7861 cgtagccgcg catcaacgtc agcggggagc gcagatcatg actcaccgta gagacaaatt
     7921 ccgatttgag ggaatccaac tctttgaaat aggtgatgtc tcgcaaaatg cacacccgtc
     7981 ccacacggat tccatcagca ataaccgatg aagctgtggc gtagtacacg cgcccatcgg
     8041 gtaaggtgac ctccacagaa cgcttatcgt cagacgagtg gatgagttcc agcaagggga
     8101 tctcagaaac tgcgcgttca atcggctgac cctcctccca ttccacgccc agtcctaagg
     8161 cgcgccaggc tgccggattg gagagcaaca agcgattttg ctgatcagtc acaagcaccg
     8221 ggtctggggt agaagccagg atagctgcca agcgctgcct gccaatctct gcattcatat
     8281 acaagcgggc attggcagcc gcaagagcgg cttgtccggc tagagtaacc tggaaacgta
     8341 tctcttcctc cccgaattta tgggattggt cataggcagt ccaaagagcg ccgtaatact
     8401 ggttttcgtg gcgcaggggt aaggccatca aagcttgtgg tcgcaagtcg tccgccttga
     8461 aattgatcaa acgcgggcga gccaggctgt gcattaccac acgctcctga ttgcgcgtca
     8521 actccaagat ctgatcatcg agataagcat acgtttcccc ggcagaaccg attgcgtaaa
     8581 cagttggaga aaccgggcta ccctccagat cgggtaccgt ctcgggagcc aaaaccaccc
     8641 tggccgagct cgcccccgag ctcagagaag cttcaagcaa aggcttgacc gcctcatcca
     8701 tctctagact agaagctact ccctggctga cgagcaaaag cctgtttaat tcatctaatc
     8761 tggctttcaa gctgaggcgc atctgctcaa aagcgcggcc taattgccca acttcatctt
     8821 caccattgaa gctgatcggg gtgtctaatt gaccctgtgc gattacccca gcctgcaccg
     8881 ccagcctctt tagggaagcg gtcaccacgc gcaaaccaaa acgaagcagc aagacggaga
     8941 atatcgctag caggacaatc atacccagca aggggccagc gatattgagc gcgatctgct
     9001 gggtacgccg cgcggggacg gtcagcacca ctgcccaagg acgccccaaa gcaggttggt
     9061 aatagactag acggcgagtg ccatctggag cggtatcgtt gtaaaattct ggtccctcag
     9121 ccgtccgccc ggtatagacc gccatcaaca aggctgggtt cgagtggtat aagattcgcc
     9181 catcttcatc cagcaagata ccctccccat cagagccagc caggctgctc aagctggcaa
     9241 taatgggttt ggtaaaggga ttgacgtcta gctctgtgcg cccaatcaag acaccccgca
     9301 cactctcatt ttcgtcgcgg attgcagcaa caaacgagac cttggaggct ttatcatcac
     9361 tatcaggtgg cacagtatat atctggtaag gcacacctgc cagggatagc tgaatcccca
     9421 tttgctcttc aggagggggt tgggcactgg catagttctc aattggataa ccagccagcg
     9481 gatttccgac ttcatccagt ataaacaact gggtgaagaa agggacactc ctcaagtctt
     9541 gcgccaggac gccgatcaga tcgtccgggt cgctcgtata caagcgcggg tcagaagaga
     9601 gctgtgcgat caggttctgc ccggcctcta ggaagaaagg cacgccttcc gaagccaaac
     9661 gagcagcatc tgccatgcgg tcgcgcaaga tctggcgggc tgccgcgccc gccaccaccc
     9721 aatcccccac catcaacaac actgtcagaa tgaacgccaa tggagcagta ccatataaaa
     9781 accgagcttg caggcttttc tctgctggag aaggcaacaa cagtccaatc ctaccccagg
     9841 cgcccggaaa tgaagccgct accacctctg agaatagacc tgccaccaac agcggaatgc
     9901 ccactgacag agaagccgcc cccaaccggg cgatggcata atccaatcga atggcaagcg
     9961 gcccatcggc gattaaagag gcactgacca aagcgatgag cgggtagaaa gccgcgatca
    10021 acagtgacgt aaacagtggg tggcgtagca acacatacga gcgcgtcctg tagcgctggt
    10081 tgatagctgc actgaaaagg attcctaaaa aggcgaattc taagggagta aacgggttgt
    10141 gcgtatccca caaacccagc aacaagccgg agaagcccgc caaggctgct gctggcacga
    10201 ttcccagaaa tccccctgcc aaggtccagg gtaccgcgat aaagaccatc atcgcaggac
    10261 caagacgatc aatggggata cccggaggtg ggagggcacc cctggcggca agccgcaaac
    10321 cgatgaataa acttgttagc ggaacaaaga cagccaaaat ggctaggatc ccccaaaaac
    10381 gggtggtacg ggaacggtga aaagcgcgcc agcgacgtaa gaacagtacc agcaacgcca
    10441 ctcccagtac ccaaaagacc cctacaatca ggcgtcccac cagactttgc ggaagatcaa
    10501 tatatgctgg ggaggtgaag agcgatagca ggaacgtcat atatcctggg cggttgcaat
    10561 taacatgcct ggatgattat agcatggata ccagaccacc acctatccat ctcgttcgtg
    10621 gcatttagac cttgtgaatg ccttgccaat catacccact cgatggtctc ctgtgtagct
    10681 tgagacaggt cagcataata tccccttcgc ctttctggaa gcatggctgc tagtatatac
    10741 atggcttcat cactcatctg gcgtagctgc tcgcggtcgg gtcgcttcag atcgggacga
    10801 aaacgaaatg gtcgtccgaa acgcacgacg gctccatcgg tcttccaagc cggcgaaaag
    10861 aaacggaggg atgggaaccc ctcggtgcct tcgatcgcca caggcacgat gggggtacct
    10921 gaatggctgg agagataggc aatgccctcc ttgccgcgag acaggtgtga agcccgcgtc
    10981 ccctcaggtg ctacgagcac catctccccc gcgcgcagca cctccaacac ttgctgcacc
    11041 gcccgccgat ccacttcctc gcggcgtact gggatcactc cccacagcct gggaaatatc
    11101 cccaccaacg ggtaattgta aacttcgatt ttggctagag ggataatatt gcgcggcatg
    11161 acgtgcaata ctacaatcgg atcgataaag gcaatatggt taatcatcaa tatcgccgga
    11221 ccctgggcgg gcacattttc tagcccttct acgcggtcca atttagccaa aagggtgaag
    11281 cctatgtact tgagcagcca gcgtaaaact cggcgacgtc tatcccaatc agtatgccga
    11341 tatttggatg aatagagcga tgttgatctt tcggacacag cttatcccca atcagagcga
    11401 tggaagaaat acaggccaga cgacatgcca aacaatatcc atccaaaaat gaataccagc
    11461 tgcggcgact aatccctgtt gcacatatcg ctcgcctgcc accatcccga caattccatt
    11521 aatcaagaaa atctcgccca tgacaagcgg tgagattacg tctaagctgg tgccgcccaa
    11581 tataaccata gccgaaccca aatgcgccgc accaaacgcc atcgcagcga tgaaatttgc
    11641 aacccaaaag gcgaccctca tcgagatcca gcgactcagg aaacggttga gcaggtacgc
    11701 ccataatccc atgacgaata cccgaaagat gatctcttca ccgatccctg ccgtggcaga
    11761 cgcaatcaga gacaaaggaa actgcgggtg tggaaaacca tcccaggcga taagtaccgc
    11821 aaatatacgg tccataacaa tcacaataaa acctagcaac aggccaatag ccattggctt
    11881 gagcagccag ccacgccagt ccgccccctc acggtaaata ccaggccagt ttaacttgcg
    11941 ggcgaaccag aatccagcca gacccaacaa accatataca acgattatca atccagcgtt
    12001 cgccaatcca tagatccaac gctggttata gtcaaagtcc ggctcaacac ctggcaattg
    12061 ggtcatttga tcgagaaacc catagtatga aataaaggtc agggctgcat aaattgccag
    12121 catgctacta aacagtgcaa gttgtctgcg agtgctcatt attcacttcg gataaacgcc
    12181 ttggtaatct agcggtaatt gctcggctaa ggtttgcatg attttctggg tgcctgcatc
    12241 aatggcctta cgccggtctg gcaggttttc cagccggaat ttagctccaa cggtcattgt
    12301 tactcgggtt ttacgcaggc gcttcatatt tgcatagatg cgcttgtttt cggttcccgt
    12361 gaacgttacg gggagaatag ggacattcgc tttgagtgcc aggtaagctg ccccaccagt
    12421 gccctcttcc agcgagccag tcaggctctc tcgtccttcc ggtgcaatgc ctaccacccg
    12481 cccttgtcgc agcgcttcca gtgcggcgcg caaggcacgg cgatctggct ggccgcggtg
    12541 cacccagatt atcccatagg cgtccattag tataccgagt attggataat cataaagctc
    12601 ggccttcgat agaacttcca cttcgtaagg tgaatacgcc atccccacaa tcaaatcggc
    12661 atcccccagg tggtttgaga ccaccaggat tggtccatga cgagggacat gctcgagccc
    12721 ttctgtgcgc atttcagtga acaaccagac caatacgcgc acgaaccatt ttatcaacct
    12781 tcggaatact cgccgccaaa acgttaagct cggcaagcgg gtgatctctg ggcgtatcaa
    12841 cgtgcttcgc ggttttgcgt caggatgaga tccgggttgt gttggcatat acaccctggt
    12901 actcaggcgg taacaatgca gcgatatgcg ccataatcag atcagcattt tcctggcgct
    12961 tgcggcgacg atcttctcct ttgccgtcca ccagtggcaa ccggaagggg gcaccgatgt
    13021 acatgtacaa tgatggcttt tctccccgca tagctcgctg aaaaaagtca tccgttgtgc
    13081 ccaccacgcc aaccgggaca accggcacat ctgtcttctc aataagataa gcgacacccg
    13141 gtgcggcacg acgcataccc ggcgcgtgcg aacgagttcc ttcaggcata atcattaagg
    13201 atttgccaga tagcaaggca gcctgggcag tattcattaa atggcggtca tattcgcccc
    13261 ggtgcaccgg gattgcacca tacatacgaa ccagaagcga ctgtccggga cgctgccaga
    13321 tatcaacagc acctaccgcc tctggttccg taggccaaaa agccagcacc aggggaggat
    13381 cgtacaatga aacatgattc gccgcaatga gatagccccc ctgccgagga atgttctcca
    13441 taccgataat attcacct
//
LOCUS       DBNGSX010000507         3402 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_258949, whole genome shotgun sequence.
ACCESSION   DBNGSX010000507 DBNGSX010000000
VERSION     DBNGSX010000507.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_258949"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..849
                     /locus_tag="AC16CB_15045"
     CDS             112..849
                     /locus_tag="AC16CB_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HIR3343837.1"
                     /translation="MSLLSRLYQSRLWRSFFRHGWPDNPLDRSLAMTSNIFLHLHPVK
                     VNRKSLKWTYSFGLGIISVILFALLVITGVLLMFYYVPSIERAYNTMKEIQYSVPLGQ
                     FTRNMHRWAAHAMVLAVFFHMIRVFYTGAYLPPREFNWVMGVILLIMTLGASFTGYLL
                     PWDQLSYWAITVGTNIAGYAPVAGQLMREFLLGGREVGQNTLIRFYTLHIAVLPLLIT
                     LFISIHIWRVRKDGGLAINDALADDQD"
     gene            925..1695
                     /locus_tag="AC16CB_15050"
     CDS             925..1695
                     /locus_tag="AC16CB_15050"
                     /inference="COORDINATES: protein motif:HMM:NF012262.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343838.1"
                     /translation="MSNNPSATDEIFPKESGKSYALVELMRGTSPMVDKGPEDTVFSF
                     PIVLLWEIILLLGVSFAIFLFSLVKQAPLDELANPMVTTDPAKAPWYFVGLQELLEHM
                     HPTLAGILIPTALVVFLIVLPYVDHSWAKPGVWFSSLRGRRITGWTALYILIVMPTYV
                     ILDNIFPMRELLRDLVPGWIAQGLLPGAILIILVMMPVLVLWRIGANRHEFMMALFTV
                     MVVSAIVFTISGFLFRGPGFKLYLPWDMPDGYNPWAEL"
     gene            1722..2219
                     /locus_tag="AC16CB_15055"
     CDS             1722..2219
                     /locus_tag="AC16CB_15055"
                     /EC_number="7.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="QcrA and Rieske domain-containing protein"
                     /protein_id="HIR3343839.1"
                     /translation="MKSNDSNNEKNIKRRDFLSFAWVITSLGVFGQAGAAIYKFLKPR
                     ILPGSFGTKVTAGQVDEFVPGTINHVQEGRFYISRLDDGGFLALWHRCTHLGCTVPWQ
                     EGEGQFHCPCHSSIFTPLGEVLSGPAPRPMDLFPIEIVDGSIIVDTSKPIERGKFDVS
                     QVTYT"
     gene            2228..3355
                     /locus_tag="AC16CB_15060"
     CDS             2228..3355
                     /locus_tag="AC16CB_15060"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3343840.1"
                     /translation="MRPKENFRPYIASAFGLTLAILVTFQTYIWREPFRIERDETLEK
                     QAKEKAGLELYSQNCVSCHGQNGEGGVGPPLNERGLLGSAPDEVLFSLIRTGVPGTLM
                     PAWSQAFGGPFTDEQIMQMVAFIRSWEPNAPLIEAGVIPPDPTRGALMYEQTCFICHG
                     ENGKGTDIAPALNDPERLGELDDAWYRKTIARGRPAKGMPTWGTVLSPMQINDLVALI
                     AAWREGEKVSSNVPMATFVTNALIAIREFDRPDSAFYLKATLPLATGEQAQEIEAILT
                     MIDENRLFEAQTRLVALLPAEEMGKASFSGNCAPCHADNGTGGIGPNLHDNSFVQSKS
                     DEEIVDFIQNGRRGTAMVGFEGILGVEEIDNLIALLREWQK"
BASE COUNT          805 a          794 c          862 g          941 t
ORIGIN      
        1 cgggctgact tactggcata cgtccagaca ctaccgacga aataataagc aattcagcag
       61 gggttttcgg acctgtcaca gtccgaaaac ccttgcaggt agaataattc tatgtcgtta
      121 ctttctcgtt tgtatcaatc tcgcctttgg cgctcctttt tccggcatgg ttggccagac
      181 aatccgcttg accgttcttt ggcaatgacc agcaatatct tcttgcacct gcacccggtc
      241 aaagtcaacc gaaaaagcct gaagtggact tactctttcg gtcttggaat catctcggtg
      301 attttatttg ccttgcttgt gatcaccggc gtcttgctta tgttctacta cgttccatca
      361 attgaacgag cgtacaacac catgaaagaa atccagtatt cggtgccatt agggcaattc
      421 acccgcaata tgcatcggtg ggctgctcac gctatggtgc tggccgtatt cttccatatg
      481 atccgtgtct tttataccgg agcatattta ccaccaagag aatttaattg ggtgatgggt
      541 gtcatacttc tcatcatgac ccttggggca agctttaccg gttacctgtt gccttgggat
      601 cagctctctt attgggcaat tacggtaggc accaatatcg ctgggtatgc acctgtagcc
      661 ggccagttga tgcgcgaatt tttactgggc gggagagaag ttggacagaa cacactgatt
      721 cgcttctaca cgcttcatat tgcagtatta cctctgctga tcactctttt catttctatc
      781 catatctggc gggtgcgtaa ggatggaggc cttgctatta atgacgcact tgcagacgac
      841 caggattgac actatatcaa gcatatctca tatgccgtaa ggcaattctg ttcttatgca
      901 tattcgtggg agttggagat cttaatgtca aataacccct ctgctactga tgagatattc
      961 cccaaagaat ctggcaaatc ttatgcactt gtcgagttga tgcgcggcac tagcccaatg
     1021 gtggataaag gaccagagga caccgttttt tcctttccaa ttgtgctcct gtgggagatc
     1081 atcttattgc ttggcgtatc atttgcgatt tttttgtttt cgttggttaa acaagcaccc
     1141 ttagatgagc tcgcaaaccc catggtgacc actgacccgg cgaaagcccc atggtatttc
     1201 gttggacttc aggagttgct tgagcatatg catcctacct tggctggtat cctcattcct
     1261 acggcgttgg tagtttttct gatcgttcta ccgtatgttg accattcctg ggcgaaacca
     1321 ggggtttggt tctcctccct acgcgggagg cgcatcacag gttggaccgc tttgtatata
     1381 ttaatagtca tgccaaccta tgtcatcttg gataacatct tcccaatgcg agaactgctt
     1441 cgagatttag tgcctggctg gatcgctcag ggattactgc cgggggcaat cttgatcata
     1501 ctggttatga tgcctgtctt ggtgctctgg cgaattgggg caaaccggca tgaatttatg
     1561 atggcattgt tcaccgtgat ggttgtttcc gctatcgtat ttactattag tggttttcta
     1621 ttccgcggac cagggtttaa gctctatctg ccttgggaca tgcccgatgg ttataaccct
     1681 tgggcagaat tgtagcttct tctcttaatc gctgaataac catgaaatca aacgactcaa
     1741 acaacgagaa aaatatcaag cggcgagatt ttcttagttt tgcgtgggtg attacatctt
     1801 taggtgtctt cgggcaagct ggagccgcca tatataaatt cctaaaaccg aggatccttc
     1861 cgggatcatt tggcaccaaa gtcactgcag gacaggtgga tgagtttgtg ccaggtacaa
     1921 tcaaccatgt gcaagaggga aggttttata tatctcgcct ggacgatggc ggtttcttgg
     1981 cgctttggca tcgttgcaca cacttgggct gcacggtgcc atggcaagaa ggagaaggtc
     2041 aattccattg tccgtgccac agttcgattt ttaccccgct tggagaggtc cttagcggcc
     2101 cggctcccag accgatggat ctcttcccca ttgagattgt agatggttcg atcatagtcg
     2161 atacgagcaa gccgatcgaa cgcggaaaat tcgatgtctc tcaggtaacc tatacttgag
     2221 gaaaataatg cgcccaaagg aaaacttccg gccttatatt gcttctgcat ttggacttac
     2281 acttgcgata ctggtcacgt tccagaccta tatctggcgt gaaccctttc gtattgaacg
     2341 cgatgagaca ctcgaaaaac aagccaaaga aaaggctggg ctagagctat attctcaaaa
     2401 ttgcgtttca tgtcatggtc aaaatggtga aggaggtgtg gggccacccc tcaatgagcg
     2461 cggcttgctg ggctcggctc cggatgaggt cttgtttagc ttaatacgca ccggtgtgcc
     2521 aggcacattg atgccggctt ggagccaggc atttggcggg cctttcacgg atgaacagat
     2581 catgcagatg gttgctttta ttcgatcctg ggaacccaat gcgcctctga ttgaggctgg
     2641 tgttattccc cctgatccaa cccgcggagc attgatgtat gagcaaacct gctttatttg
     2701 tcatggtgag aatggaaaag ggacggatat cgctccagcg ttgaacgacc cagagcgcct
     2761 tggcgagtta gatgatgctt ggtatcgtaa aaccattgcc agaggacggc ctgccaaagg
     2821 gatgcccacc tgggggactg tgctttcacc gatgcaaata aacgatctgg ttgctctaat
     2881 agcagcatgg cgtgaggggg aaaaagtgag ctcaaatgtg cccatggcga cttttgttac
     2941 caatgcccta atcgcgatcc gtgaattcga ccgtccggac tcggcgtttt acctgaaggc
     3001 aaccctgccg ctggccaccg gcgagcaggc tcaagagatc gaagctatcc tcacgatgat
     3061 agacgaaaac cgtctgtttg aggcgcaaac tcgtttagtc gctctcctgc ctgctgaaga
     3121 gatggggaaa gcgtctttca gcggtaattg tgccccatgc catgctgaca atggaacagg
     3181 ggggattggg ccaaaccttc acgacaattc atttgtgcaa tcgaaaagcg atgaagagat
     3241 agttgatttt atacagaatg gtcgcagagg gacggcaatg gttggctttg aaggcatcct
     3301 gggggttgaa gagatcgaca atttgattgc tttgctacgt gagtggcaaa aatagaacaa
     3361 tcaggcagga acgcaccgtc atgcgtaaca tggtgtgatg gt
//
LOCUS       DBNGSX010000508         2379 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1228758, whole genome shotgun sequence.
ACCESSION   DBNGSX010000508 DBNGSX010000000
VERSION     DBNGSX010000508.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1228758"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /gene="cofE"
                     /locus_tag="AC16CB_15065"
     CDS             complement(<1..638)
                     /gene="cofE"
                     /locus_tag="AC16CB_15065"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HIR3343841.1"
                     /translation="MPLTLTPLPGIPLIQPGDDLAEIIMQSLGREEIVLQDGDILVLA
                     QKIVSKSQGRRVNLATINPSQTALELASQTDKDPRFLELVLRESSEVLRTRPGSIIVE
                     HRLGFVCANAGIDHSNVAGESIQDEEWVLLLPEEPDNAAEAIRRCLEAASGVRLGVLI
                     IDSHGRAWRLGTVGVSIGLSGLPALVDLRGQEDLFGYKLRITQVGAADELAAA"
     gene            complement(675..1334)
                     /gene="npdG"
                     /locus_tag="AC16CB_15070"
     CDS             complement(675..1334)
                     /gene="npdG"
                     /locus_tag="AC16CB_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0006740 - NADPH regeneration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HIR3343842.1"
                     /translation="MTETLPIIAILGGTGKEGPGLALRWGGAGYPIIIGSRQTEKAQA
                     TADELNAMLGGNSVQGMENGLAARNADICVLTVMYTAHQSAIDSLIDDLQGKILVDAT
                     ARVDFRDPRPPDPPSAARIAQDKLGTGVRVVAAFQNVPAHALKKNLGQPLDSDVLVCA
                     DDVEAANEVIKLAEAGGMRAYYAGGLDNAIVVEGLTSIMISLNKHHGVKTASIGITGI
                     R"
     gene            complement(1348..>2379)
                     /locus_tag="AC16CB_15075"
     CDS             complement(1348..>2379)
                     /locus_tag="AC16CB_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3343843.1"
                     /translation="RASAEVMLVDAIKHGTTTLIDHHASPNAIEGSLDTIAEAVDRCG
                     LRAVLCYEVTDRNGLDGARRGIDENMRFIKEHSSGGSGSSKMAALFGLHASLTLSDET
                     LDACRAAAPKGTGFHIHVAEHEADEYDSLAKSGLRVVDRLHRRGILGPRTIVAHAVHV
                     DAREAGLLADSGTWVTHQPRSNMNNGVGVAEVESLMRLGVPVCMGTDGFSHTMWVEWK
                     TTYLLHKVWHRDPRRMNGMDVVAMGVYNNAALAGGFFPQGALGVIKPGAHADLIFVDY
                     QPHTPLTSDNVPWHIIFGFHESMVTTTIVAGQVLMRDRQLLTLDEKAIAARALEQAPG
                     VWERYNAQF"
BASE COUNT          519 a          722 c          636 g          502 t
ORIGIN      
        1 gcggcagcca gctcgtcagc agcgccaact tgggtgatgc gcagcttata accgaacaaa
       61 tcctcctggc cgcgcaggtc gaccagagct ggtagacccg agaggccaat cgaaaccccc
      121 accgtcccca aacgccaggc gcgcccatgt gaatcgatga tcaaaacccc taatcgcacc
      181 ccgctggcag cttccaagca gcggcggatt gcctcagcgg cgttgtctgg ttcctcaggc
      241 aatagcaaga cccactcctc atcctggatg ctctccccgg ctacattgga gtgatcgata
      301 cctgcattgg cgcagacaaa acccaggcgg tgttcgacaa taatcgatcc ggggcgcgta
      361 cgaagcactt cgctgctctc ccgcagaacc aactccaaaa aacgggggtc cttatctgtt
      421 tgtgaagcca gctcaagagc tgtttgcgat ggattgatgg tcgccagatt taccctgcgt
      481 ccctggctct tggagacaat cttttgtgcc aggacgagta tatcgccgtc ctgcagcaca
      541 atctcttccc gccccaggga ttgcatgatg atctcagcca agtcatcccc aggttggatg
      601 agggggatac cgggaagggg tgttagcgtt aggggcatat cagaagataa gcaacagtag
      661 aacccttcta gtttttatcg gatccctgtt attccaatac tggcagtctt aacgccatgg
      721 tgcttgttca ggctgatcat aatcgaggtt aagccctcga caacaatggc gttatcaagc
      781 ccgccggcgt aataggcgcg catgcctcca gcctcagcca gtttgatcac ctcattggca
      841 gcctctacgt catcagcgca caccaggaca tcggaatcca atggctgccc caggtttttt
      901 ttcagggcgt gagccggcac attctggaag gcagccacca ccctgacacc cgtcccaagc
      961 ttatcttggg caatgcgcgc agccgagggc gggtcaggcg ggcgaggatc gcgaaaatcc
     1021 acgcgtgcag tagcatcaac cagtatcttg ccctgtagat cgtctatcaa gctgtcaatg
     1081 gctgactggt gggcagtgta catcacggtc agcacacaga tatcagcatt acgcgctgca
     1141 agaccgtttt ccatgccctg aacggagttc ccgccaagca tggcatttaa ttcatcagca
     1201 gtcgcctgtg ctttttccgt ttgccgagag ccgatgatga tggggtaacc agcgccgccc
     1261 catcgaagtg ccagccctgg cccttctttc ccagtcccac ctaagatagc gattattggt
     1321 aatgtttccg tcataaattt ccctgtttca gaactgcgcg ttatagcgtt cccacacccc
     1381 aggagcttgc tcaagggcac gggcggcgat agctttctca tccagtgtga gcaactggcg
     1441 atcccgcatc aaaacctgac ctgccacgat agtggtggtc accatgctct cgtgaaagcc
     1501 aaagataata tgccagggaa cgttgtccga ggtgagcggc gtatgcggct ggtaatcaac
     1561 aaaaatcagg tcggcatgag caccgggctt gatcacgccc aaagccccct gcgggaagaa
     1621 acctcctgcc aaagcggcgt tgttatacac gcccatggca accacgtcca tcccattcat
     1681 gcggcgcgga tcgcggtgcc acaccttgtg cagcaggtag gttgttttcc actccaccca
     1741 catggtatgg gagaagccgt ccgtacccat gcacaccggt acgccgagcc tcatcaaaga
     1801 ctccacttcc gccactccca cgccgttatt catgttggag cgaggctggt gggtgaccca
     1861 tgtgccgcta tctgccagca aaccggcctc gcgagcgtcc acgtgcactg cgtgggccac
     1921 gatggtgcgc ggaccgagaa tacctcgccg gtgcaaacgg tcgaccacac gcaaacctga
     1981 tttggcgagg ctgtcgtact catcggcctc atgctcggcg acgtggatat ggaatccggt
     2041 ccctttcgga gctgcagcgc ggcacgcatc caatgtctcg tctgagaggg tcaggctggc
     2101 gtgaagcccg aagagggctg ccatcttgga tgatcctgaa ccgcccgagg agtgttcctt
     2161 gatgaagcgc atattttcat cgatgccacg ccgcgcccca tccagaccgt ttcggtctgt
     2221 cacctcatag catagcacgg cccgtaaacc gcagcgatct accgcttcgg cgatggtatc
     2281 cagcgagcct tcgatggcat taggcgaagc gtgatggtcg atgagcgtcg tcgtgccgtg
     2341 tttaatggca tccaccagca tcacttctgc cgaagcacg
//
LOCUS       DBNGSX010000509         6826 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1505898, whole genome shotgun sequence.
ACCESSION   DBNGSX010000509 DBNGSX010000000
VERSION     DBNGSX010000509.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6826)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1505898"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="AC16CB_15080"
     CDS             <1..1154
                     /locus_tag="AC16CB_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012628804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR3343844.1"
                     /translation="SLSRRAFLRITTALGGAAALISFLEGCSKAEIAQETLISPTDTL
                     EISTLTQEVLPTTSPTQEEIQPTEATAVPSATSTESVENGVARLAFVKTTDRAEGVRR
                     AIDLLGINPVAGKHVFLKPNFNSSDPAPGSTHPEVLVALVKALKEMGAKEITVGDRSG
                     MGDSRRVMDRIGVFEMADELGFETIVFDELQAEDWVMIQPPGSHWENGFPFARPCLEA
                     EALVQTCCLKTHRFGGHFTMSLKNSVGMVAKKLPRDDHDFMQELHFSSNQRRMIAEIN
                     AAYAPALIVLDGIEAFISGGPDKGERASPEVVLAGTDRIAIDAVGIALLRYFGCKTEV
                     ARGKIFQQEQIARAVDLGLGVDHPEKIEFLTADTDSAAYSEKIKEVLLA"
     gene            1297..2019
                     /locus_tag="AC16CB_15085"
     CDS             1297..2019
                     /locus_tag="AC16CB_15085"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3343845.1"
                     /translation="MKPEGSTPMGDVGDQTIIQATNIYKTYYTGTVAVPALRGVNLCV
                     RRGEMVAVMGPSGCGKTTLLNCLSGLDYVDSGEIIIEGTSLAKMSDRERTDYRARRMG
                     FVFQFYNLLPVLSAVENVELPIGVAGVRPSEARERALEALAQVGLADRADHRPAAISG
                     GERQRTTIARALANRPAIVWADEPTGDLDSETATDIMDLLLRLNVEAGLTFILVTHDR
                     QVGARCGRMVRMRDGLIENETS"
     gene            2022..5405
                     /locus_tag="AC16CB_15090"
     CDS             2022..5405
                     /locus_tag="AC16CB_15090"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3343846.1"
                     /translation="MEKLFGLEMNMIAGCLGSSLALVIVVLALLALRERVMFKLGIRP
                     IARRRTQSALIVFGLMLATLIITAAFFMGDTMSHTIRSLTLDELGEVDEIIRLSGAGS
                     GTTQQTYFKFARYEELVTALEDFEMIDFFVPAVRESIPVVNTTRRKSERSLTVIGLRQ
                     SDTAILENEELIDVEGKPLNLTQLGPREVYLNAAAAQDLNASPGDKLELYTESKPKIV
                     QVRSVTAGGKSPRMFLPLRRAQTLFKQRGRINEIFISNIGDASTGANLSQPVTTHLRG
                     LLTDRVVAGRIVNLLLRDASILGTLRGEASDLRGNIQLDLLALTDFLETGVLSNELLS
                     LLADEEVADEVQVILDQAEWMNQPSRDRLAELFVDLSELEVVDVKRDNLDQGELAASA
                     FTTIFIVTGLFGIAAGLLLIFLIFVMLAAERKPEMGMTRAIGGQRMHLVEMFVFEGTA
                     YDLGAAAVGVGLGIVTGYVLAASLGQAFAAQSDLAIRPNLTIRSMIVSYSLGMLVTFG
                     TVLFSAYKVSKLNIVAAVRDLPEPPSPPTRLRDRLFAPFRVLGEGFRHLRRLRIGRAL
                     WTFWVQAPLSLLGVVWAGLIGGPLTLLVGLFLTPAGVAQSSGAAFSLGGSFLIIGVCL
                     TLRGLLSLALRKRPGLADRLAFTLLGLGLVIFWSLPSGFFEPLGVKDLKSGPEMLFIS
                     GILLVAGAVLLVMYNTDLLLKIILRLTGGARRLTPVLRMSIAYPLANRFRTGLTMGMF
                     AVVVFSVIFMATAFKVNESFFADTDALTGGFDLRTESSRTNPVPDLEAAIASNRDINQ
                     RDFLVVADRSSLGIEARQGRGQWRDYLIYGVDDDYLENVAFDLTVMAEGYESGAEVWR
                     AVRDERGYAVIDSFGVPSRQTTSIVIGAPDFKLEGVYIEDESMQPITIEVRDSFSEAT
                     FEVTVIGVLEPGANLGLYTSQRTLERELPYELVSTTHFIRLAGGVDRAAAGAALEGAF
                     VKHGLQSIDQIEELKDAQASQRVIEVLLQGFLTLGLVVGVAALGVVSTRAVVERRQQI
                     GMLRALGFQRQMVSWSFMIESSFVALLGISMGTVLALIPAYHMINEMAQEYPSIKFQV
                     PWDSIIVVVGLAYGMALLATWLPARQASRVAPAEALRYE"
     gene            5863..6360
                     /locus_tag="AC16CB_15095"
     CDS             5863..6360
                     /locus_tag="AC16CB_15095"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HIR3343847.1"
                     /translation="MSSVTRICFVCLGNIVRSPLAKSLFLYHANQAGRGDLYEVDSAA
                     TSGWHVGESPDPRMRRVSARHGVPLDGRARQFKKPDFERFDLILVMDGENYSNLRRLG
                     PSPEQLQKVHFLRDFEPSGGSRLEVPDPYYAGIDGFETVFEIVDRSVRGLLDVLENNT
                     PVKRR"
     gene            6423..>6826
                     /locus_tag="AC16CB_15100"
     CDS             6423..>6826
                     /locus_tag="AC16CB_15100"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015818.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HIR3343848.1"
                     /translation="MLPEPVSDWLKAQACGQVVSVRQVGGGCINNGARLQTTSGRTFF
                     LKTNRSTPADMFAREAEGLEALAVNGGPRVPTPYLYGADFLLLEDLAPTPRQKEYWPE
                     FGRCLATLHSHTQVRYGFQHDNYIGSTPQSNT"
BASE COUNT         1448 a         1707 c         2028 g         1643 t
ORIGIN      
        1 tgtctttgag tagaagggcc ttcctgcgca ttaccacagc cctgggtgga gcagctgctc
       61 tgatttcatt cctggaggga tgttcaaagg cggagatagc tcaggagaca ttgatctctc
      121 ctaccgatac acttgaaatt tcaacgctca cccaggaagt attaccgaca acttcaccta
      181 cgcaagaaga aatccagcca actgaggcca ctgctgttcc ttctgcaacc tccaccgaat
      241 cggtggaaaa tggcgtggct cgcttggctt tcgtcaaaac aactgatcga gctgaaggtg
      301 tgcgcagagc gattgacctg ttagggatca atcctgttgc ggggaaacat gttttcctga
      361 agccgaattt caacagctca gacccggcac caggctcaac ccacccggaa gttctcgtcg
      421 cgctcgtgaa ggcgctcaaa gagatgggtg caaaggagat tacggttggc gaccggagtg
      481 gtatggggga ttcgcgccgg gttatggata ggatcggcgt ttttgagatg gcagatgaat
      541 tgggatttga gacgatagtc tttgatgagc tgcaagccga agattgggta atgatccaac
      601 caccaggcag tcattgggaa aacggtttcc catttgcgcg cccgtgtctc gaagcagagg
      661 cgctcgtgca gacgtgctgt ttgaaaaccc acaggtttgg aggtcacttc accatgtcgc
      721 tgaagaattc ggtgggtatg gtggctaaga agctcccgcg agatgatcat gattttatgc
      781 aggagcttca tttttcctct aaccagagaa ggatgatcgc ggaaatcaac gcggcctacg
      841 cgccggcgtt gatcgttttg gatgggatcg aagcatttat ttctggtggg ccggataagg
      901 gtgagcgcgc ctcccctgag gtagttttgg cggggacgga ccgcatcgct attgacgcgg
      961 tcggtattgc cctattgcgc tacttcggtt gcaagacgga ggtcgccagg ggaaagatat
     1021 tccagcaaga gcaaattgca agggcggttg acctgggatt aggagtggac catccagaga
     1081 agattgaatt tctgaccgca gatacagaca gcgcagccta cagtgaaaaa atcaaggagg
     1141 ttttactggc gtgataatcg tgtaaacata cagttgctag ttatgcacac gaggatgttg
     1201 attgaaactg ggagggaatc aagatgtgac acagggttac tctcaaccat catttaagtt
     1261 gatggtaaat ttgaaagaga agtggatggg acgggaatga agcccgaagg aagcacaccc
     1321 atgggcgacg ttggcgatca aacgattata caggcgacaa atatctataa aacttattac
     1381 accggaaccg ttgctgtacc tgcgttgcgt ggtgtcaacc tgtgtgtcag gcgcggtgag
     1441 atggtggccg tgatgggacc ctctgggtgt ggtaagacaa cactgctgaa ttgcttgtct
     1501 gggctggatt acgtcgacag cggggagatc attatcgagg gcacttcatt ggcgaagatg
     1561 tccgaccggg agcgtactga ctatcgcgcc cggcgcatgg gttttgtctt ccaattctac
     1621 aacctgctgc ccgtcttgag cgcggtagaa aacgtcgagc taccaatagg ggtagctgga
     1681 gtccgcccaa gcgaagctcg cgagcgagcg cttgaagcat tggctcaggt tggccttgca
     1741 gaccgtgcgg atcaccgccc cgcggcgatc tccggaggcg agcgccaacg aacaaccatc
     1801 gcccgagctc tggctaaccg tccagcaatt gtatgggcgg atgaacccac cggcgattta
     1861 gattccgaaa ccgccacaga catcatggac ttgctgttgc gtctgaacgt cgaagctgga
     1921 ttgaccttta tcctggtgac ccacgatcgt caggtaggcg cgcgttgcgg tcgtatggtg
     1981 cgcatgcgcg atggcctgat cgaaaacgaa acgagttagc ggtggaaaag ctcttcggct
     2041 tagaaatgaa tatgatcgcc ggttgcctgg gatcgagcct agcactagtg atcgttgtct
     2101 tggccttgct ggccttgcgt gagcgggtca tgttcaaatt ggggatacgc cccatcgcgc
     2161 gacgccgcac gcaatctgcc cttatcgttt ttggtttgat gttggccacg ctgatcatca
     2221 ccgcagcttt cttcatgggc gacacgatgt ctcacacgat tcgctcacta accctagacg
     2281 agttaggcga ggtagatgaa ataatacgcc tgagtggcgc aggtagcgga actacacagc
     2341 agacctactt caaatttgcc cgctatgagg aattggtgac ggcattggaa gacttcgaga
     2401 tgatcgattt cttcgtgcct gccgtacgcg agtcgattcc ggtggtcaat accacgcggc
     2461 gcaaaagtga gcggtcttta accgtgatcg gtctgcgtca aagtgacact gccatcctcg
     2521 aaaacgagga gctgatcgac gtggagggaa aacctctcaa cctaacacaa ctgggaccgc
     2581 gcgaagtgta cctcaacgcg gccgcagctc aagacctaaa cgcctcaccg ggcgataaac
     2641 ttgagctgta taccgaatcg aaaccaaaga tcgtccaggt gcgcagtgtt actgccggtg
     2701 gtaagagccc gcgcatgttt ttacccctgc ggcgtgcgca gacgcttttc aaacaacgag
     2761 gtaggatcaa cgagatcttc atctccaata tcggggatgc ttccactgga gcaaacctca
     2821 gccaaccggt cacaacgcac ttgcgtggct tgctgacaga tcgagtggtt gcaggcagga
     2881 ttgttaattt actcttgcgt gacgcgtcga tattgggaac tttgcgcggg gaagccagcg
     2941 acctgcgcgg gaacatccag ctagatttat tagccttaac cgatttcctg gagaccggcg
     3001 tgttatccaa tgagttactg agcttgctgg ctgacgagga ggttgccgac gaggtgcagg
     3061 tcatcttgga ccaggccgag tggatgaatc aaccctcacg cgatcggctg gctgaactgt
     3121 ttgtggatct gtccgagttg gaagtggttg atgtgaaacg cgacaacctg gaccagggtg
     3181 agttagccgc cagcgccttc accacgatct tcattgtgac cggtcttttc ggcatcgcag
     3241 caggtctgtt gttgatattt cttatctttg tcatgttagc agccgaacga aaacctgaga
     3301 tgggcatgac ccgtgccatt ggcggccaac gtatgcacct ggtggagatg ttcgtgtttg
     3361 aagggacagc ctacgacctg ggtgcagctg cggttggagt agggctggga attgtgactg
     3421 ggtacgtgct tgccgccagc ctgggtcagg catttgcagc ccaatctgat cttgccattc
     3481 ggcctaactt gacgatccgc agcatgatcg tctcttactc acttggcatg ctggtgacct
     3541 tcggtactgt tctcttttcg gcctacaaag tcagcaagct gaatattgta gcggccgtcc
     3601 gtgacctccc ggaaccgccc tcaccaccca ctcgattgcg ggaccgcctc tttgctcctt
     3661 tccgcgtcct tggggagggc tttcgccacc tgcggcgctt gcggatcggg cgcgccctat
     3721 ggaccttttg ggtgcaagcg ccgctcagcc tgctaggggt cgtgtgggcg ggtctcatcg
     3781 gtgggccgct gacgctgctt gtaggtttgt tcctgacacc cgctggcgtg gcacaatcca
     3841 gcggggcggc attttctctg ggcggctcgt tcctcatcat aggcgtgtgt ttgactttac
     3901 ggggcttgct ctcgcttgcg ctgcgcaagc gacccgggct ggcggaccgg ctcgcattca
     3961 ccttgctggg tctcgggctg gttatctttt ggtccctgcc gtctggtttc tttgaacccc
     4021 tgggcgtcaa agacctgaaa tctgggccgg agatgctgtt catttccggg atcctgctgg
     4081 tggctggggc tgtgctgctg gtgatgtaca acaccgatct gctcctgaag atcatcctgc
     4141 ggctgaccgg cggagcccgg cgtctgactc cggttttacg tatgtccatc gcttaccccc
     4201 tggctaaccg cttccgcact ggcctgacca tgggcatgtt cgctgtggtg gtcttcagtg
     4261 tcatcttcat ggccactgca tttaaggtta atgagagctt tttcgcagac accgatgcct
     4321 tgactggcgg ttttgacctg cgcacggaat ctagccgcac caaccccgtg ccggacctgg
     4381 aggcggctat cgcctccaac agggatatta accagaggga ctttttggtc gttgcggatc
     4441 gttccagcct gggcatagag gcgcgccagg ggcgggggca gtggagggac tacctgatct
     4501 acggagtaga tgacgactac ctggagaacg tggcctttga cctgacggtg atggcagaag
     4561 gatacgagtc gggggctgag gtatggcgcg ccgtgcgcga cgagcgcggc tatgccgtca
     4621 ttgatagttt cggtgtgccc tcccgccaga cgaccagcat cgtgattggc gcgcctgatt
     4681 tcaagctgga gggggtctat atcgaagacg aaagcatgca accaataacc atcgaagtac
     4741 gcgactcctt cagtgaggcg acctttgagg tgacggttat cggtgtcctt gaaccgggag
     4801 ctaacctggg cctgtacacc tcgcaaagga cgttggaacg ggagttgcct tacgagttgg
     4861 tatccacgac gcactttatc cggctggctg ggggtgttga tcgggcggca gcaggggctg
     4921 cgctggaagg cgcatttgtg aaacacggcc tgcagagcat tgaccagatc gaagaattga
     4981 aagacgcgca ggccagccag cgcgtaatcg aagtcttgct ccaggggttc ttaactttgg
     5041 gactggtggt gggtgtagct gcactggggg tggttagcac ccgggcagtg gtggagcgtc
     5101 gtcagcaaat cgggatgttg cgagcgctag gctttcagcg ccagatggtc agctggagtt
     5161 tcatgattga gtcatctttc gtggcgttgc tgggtatcag tatgggcacg gtgctggcgc
     5221 taatcccagc ctatcacatg atcaacgaaa tggcgcaaga gtaccccagt attaagttcc
     5281 aggtaccctg ggattctatt atcgtggtgg ttggcctcgc ctatgggatg gctctgctgg
     5341 cgacatggct gcctgcccgt caggcatctc gcgtggctcc tgcggaagcc ttgcgctacg
     5401 agtaaagctt gcgatgcttt ctacatagta ctaacattgg gagatatatt ccaatagaat
     5461 aatgaaagaa attcaacagc taatggctgg cagggcaatg gcatatgccc tgaggggcat
     5521 gcgcaatccc gtaaaaagag tgtttggggt gttttggtgg aggtataccc ccaccaaaac
     5581 accccatttt ctctcctcag tgttgggaaa tcgcccaaat gcgattgccc tatggctggt
     5641 cggtagccgt aatgggacgg gtgtatttgc gcttcggttc cagaagaaac tctaattcat
     5701 atccttgcag ttctatcacg tcggtgcgct tgatcgctgt tgcaattatt tgattagctc
     5761 aaatggagaa tagatcccga aaatattagc gggcggcata atcgcccgtt atttaatttc
     5821 gggtacaatg gtgcgtaaat tgtttgtttt tggaggttac atttgtcgtc tgttacacgt
     5881 atttgttttg tctgtctggg caatatcgtg cgcagcccgc tggcgaaaag cttgtttctt
     5941 taccatgcca atcaggcggg tagaggagat ctttatgagg tcgattcggc tgccacttcg
     6001 ggctggcatg tcggcgagtc gcccgacccg cgcatgcgcc gggtgtctgc ccggcatggt
     6061 gtgccgcttg atggtcgcgc tcgccagttt aaaaagcctg atttcgagcg ttttgacctg
     6121 atcctggtca tggatggcga aaattacagc aacctgcgcc gactgggtcc ctccccagag
     6181 caattgcaga aagtacattt cttgcgcgat ttcgagccct cgggcggatc gcgattggaa
     6241 gttcctgatc catattacgc tggtatcgat gggttcgaga cggtttttga gatcgttgat
     6301 cgctcggtgc gtggattatt ggatgtattg gagaacaata ccccggtcaa gcgcagataa
     6361 actttccgtg acattggccg aaatgacctg ctgtactact caatgtaaat tggatggtga
     6421 atatgcttcc tgagcctgtg agcgactggt tgaaagcgca agcttgtggt caggtggtct
     6481 cggtgcggca ggtggggggc ggctgcatca acaatggagc gcgcctgcag acgacttctg
     6541 gtaggacgtt tttcttaaag accaaccgct ctacaccagc agatatgttc gcccgtgaag
     6601 cggaggggtt ggaggcgcta gccgtaaatg gtgggccgcg cgttcccaca ccctacttgt
     6661 atggagcgga ttttcttttg ctggaggatc ttgcaccgac gcctcgccaa aaggaatact
     6721 ggcctgagtt cggccgctgc ttggcgacac tccatagcca cacccaagtg cgctatggct
     6781 tccagcacga taactatatt ggaagcacgc cccagtccaa tacctg
//
LOCUS       DBNGSX010000510        13720 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1298598, whole genome shotgun sequence.
ACCESSION   DBNGSX010000510 DBNGSX010000000
VERSION     DBNGSX010000510.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13720)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13720)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13720
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1298598"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="AC16CB_15105"
     CDS             <1..669
                     /locus_tag="AC16CB_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343849.1"
                     /translation="YFTTFAIANPLIFRWYLTPPLPAYFLIILVGIERITHQIFNSRK
                     SRISFPNISKTRIGNILSIAFVVIAPYFLTLRGWTFHPDHGPTRPAPEMAYIQLELFY
                     HQAAESLNADISSHSTPPLLAAGDVGILGYYTGARILDTVGLNSPVSTSYFPLDPALY
                     VINYAIPPDLILDQRPDYVVILEVYGREGLLKDPRFQEAYHLRQKILTDMYGSDGMLI
                     YERK"
     gene            complement(729..1943)
                     /locus_tag="AC16CB_15110"
     CDS             complement(729..1943)
                     /locus_tag="AC16CB_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3343850.1"
                     /translation="MIDEDLLSAIEDREEEHPAIARLIELGRQKSYVTIDDILSFFPD
                     AETDVDQLEEAFAALISAGIAYMDEPVAAGPSDDELAEEEIEEESQRDLSIDDNYLAN
                     IDTDDTIGLYLKEVGRVPLLTAREEVELAQRIEAGRLAREELARGNVSSRRRVELQLL
                     IEDGWAAREHLITANSRLVISVAKKYMGRGVPFLDLIQEGNIGLIRAAKKFDYRRGHK
                     FSTYATWWIRQAVTRAIADQGRTIRVPVHMGDQINKLLRVQHQLTQRLGRDPTVEELA
                     GALDVTPQKVENMIQVARRPLSLETPTDDEEDSVLGDFIQDEEVAAPDETATYNLLRE
                     HLDNVLNGLPPREVRILQLRYGLLDGQAYTLEEVGRKMGVTRERVRQIEAQALSRLRH
                     PAIRRKLREYLS"
     gene            complement(2025..2633)
                     /locus_tag="AC16CB_15115"
     CDS             complement(2025..2633)
                     /locus_tag="AC16CB_15115"
                     /inference="COORDINATES: protein motif:HMM:NF025598.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HIR3343851.1"
                     /translation="MIRRPTWVLLALFIVILAAALLWQRSEENKSASEPTPTLPARLL
                     DIDANTIRDIKIDDDQNNRLFLRKLGGGLWIMTEPERQNLDLEELNARVTQLSLINIM
                     TTLQPAPDADEIGLLPPAYTLTITDEAGKKYILDVGSETPTQSGYYVRMNGSIYVVSK
                     FNIDGMIEMLENPPIAPPTGTPTGAVAPQEVITGTAVTTTTP"
     gene            complement(2688..4241)
                     /locus_tag="AC16CB_15120"
     CDS             complement(2688..4241)
                     /locus_tag="AC16CB_15120"
                     /inference="COORDINATES: protein motif:HMM:NF021351.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HIR3343852.1"
                     /translation="MKPEWRRFAPVGLYLAILAALTSISLYVIQREWNLYLQISLGVV
                     LLGLAIFALLDPGRVRTALTGRQARYGSNALVMAVAFVGILVVVNYLGYKNSKRWDLT
                     EDKQNTLATETLDTLANLPEPVEALGFFTARISSDSAENLLEKYKFEGKGKFNYRFID
                     PEADPIAAKDAKITRDGMIVMKMGDRQESLEFVSEKEMTSALVRLISGETRSVYFLTG
                     HGERDPEGVGDEAYSLVKLVLEGKNYVTGKLNLLSTANIPDDATVIVIAGPQQPLANE
                     EVEKLKAFVESGGGLVVMEEPLVLTDFADRPDPLATYLSESFGIVLGNDIVVDFTSQQ
                     PFVAVANSYASHPITQKMQGLVSLFPTTRSVSVTGEFNDLNRTELVLTAENAWAETDL
                     QSLQGGEQIELQPDPGVDMLGPVSLAVVIESLGKQNRLIVYGDADFANDANFMQYGNG
                     DLLINSIDWAAGQENLINLTPKDSTQRVLIPPGRYTMGLILFGFVFLVPGAVLFSGIV
                     VWIQRRKRG"
     gene            complement(4241..4975)
                     /locus_tag="AC16CB_15125"
     CDS             complement(4241..4975)
                     /locus_tag="AC16CB_15125"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3343853.1"
                     /translation="MRNVWTIANREYKLYFASPVAYMVAFFLLLILGIIFYVNVAGAI
                     VQQFPPNIQGVLGPLMTLLLFTTPAITMRLLAEEQRLGTMELLLTAPVRDWELVVGKW
                     LGGFLFLLTILAVTWLYPIFLNQMVDPGIDQGPLITGYLGVILFTASLVAIGVAVSSL
                     FSNQIAVFFATVGVYLLLWLISLPSQASGSATANELLRYLDFSDHFYNTFYQGIIDVS
                     DVIYYLSLTALALFLGSVSVESRRWR"
     gene            complement(4980..5957)
                     /locus_tag="AC16CB_15130"
     CDS             complement(4980..5957)
                     /locus_tag="AC16CB_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006198404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3343854.1"
                     /translation="MILVEGLTKDYGSRRAIDTLNFHAENGEILGFLGPNGAGKTTTM
                     RILTGYMPPTSGRATVAGFDVVEQSLDVRRRVGYLPETVPLYPEMTVFDYLKFMADLR
                     KLNNAEDRVYDVLDLVHMTDRAEGYIANLSKGMRQRIGVAQALLHEPEVLILDEPTIG
                     LDPAQILEVRNMIRELGRERTVLLSTHILSEAQQVCNRVLIINKGRIVAEDTPENLQS
                     RLAGAKRVSLRVRGDVEGLEELLAKIPGVLRIFTSGDGGLEFETAPGQDVRPEVARTA
                     VNQGYDLLELRSIGLSLEDIFLQLTREEPEPPSMAADDDFVQAEEGEEE"
     gene            6268..8589
                     /locus_tag="AC16CB_15135"
     CDS             6268..8589
                     /locus_tag="AC16CB_15135"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HIR3343855.1"
                     /translation="MNILGLARHGLNDFEQIAIIAVVITAFISLIYAWLLRGNVLNKD
                     KGTEKMQEVWNAIRIGADSYLSRQLRSILPLIGVLTVALFLSVYVVPPSREAINEFGP
                     ENAQIIVAIGRTVAFIMGASFSLIVGQLGMRMAIQASVRAASASRRGFNESLSIAYYA
                     GTITGMLTDGLGLFGGTIIFIIFGTAAPDALLGFGFGGTLIALFMRVGGGIYTKAADV
                     GADLVGKVEKDIPEDDPRNAAVVADLVGDNVGDCAGMAADIFESYEVTIVSGLILGLT
                     LVAIDPTHSLKWIVYPLIIRAIGVISSILGTFTVPIWERFPIKFLRAHDAEEAMFRSY
                     EVSSVNTIIWSFLLAFLYANDWKLAMLTTIGVGLAVAFNPLTSYFTSTKSPPVKEIVS
                     STRTGPATTILSGLSVGMESSVWALVVIVVSFIFALLLYGGQGSIYVLYAVAMIGIGM
                     LSHTGNNVAMDSYGPISDNANGIGEMAWHDLEDESTMKARQIMADLDAVGNTTKAITK
                     GIAIASAVIAAVSLFASYITDVGRVQEQLGVPMMESIRVSDTRVFIGLLLGGALPWLF
                     SSLSIRAVSRAAGLIVLEVRRQFRIPGIMEGKVKPDYARVVEISTAAAQKELIPLAVI
                     SVMLPLAVGLVLQVEALGGFLAGIILSGQLLAVFMSNAGGAWDNAKKSIEDEDRDLEA
                     NTGKGSERHKAGVVGDTVGDPLKDTAGPALNPMIKVTNLVSLIAAPIIVKYSSPNAIV
                     IVVVVLLLASIVWAINQSKKPAEEISQEVVPGD"
     gene            8790..12404
                     /locus_tag="AC16CB_15140"
     CDS             8790..12404
                     /locus_tag="AC16CB_15140"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3343856.1"
                     /translation="MAKIPLRAYNREIEKLIDQNQLEQAIAHARHILNYFPKHVNSYR
                     LLGKAYLESQRYGDAADVFQRVLSSIPDDFISHVGMSIIREDEANLDHAIWHMERAFE
                     VQPANGAIQGELRRLYGRRDGMEPPKVRLTRSALARMYIKGELFQQAISEVRAALAED
                     PQRPDLLSLLAQAYFQNGQRVEAANTCSSLLNKFPYSLEANRILAEILSSTERAEEAQ
                     VYRQRVQALEPYFGHTSPASPTPDLVSETAVVLEKLVWSPGQSVTGAQKQPEWAASLG
                     VELEATAPAEEGPPEWLADQPEAEPSATPEAHEEGDEAPSETPTPPSETLFAELSEDA
                     IPDWMKEAGWQSASGDVEEPVDILEGPAQDELTPAQIPDWLQSIAPGEKPEAEISDET
                     GESMPPIEETLSDSDALSWLDDAPPGPSDTVASWLDENQPAVPDISETESETEEKTEV
                     PAWLKDLQQPEPPSVSDETLTGTLTSPDEVSELEPQAQSLLPEIEAESDFAPSTTEAD
                     EGEKIPDWLQELGGAPSVKEEIEPTEEQPDWLRQSDQEEWQTSEPSETATPETLSMPP
                     VEELPISSEDDELAWLDDLADKQGKKESIPLTPEDALAEAPDWVLEAALETPEILPTA
                     EDAPTSPLSELPTEVADQDTEDLQLPDQVEPIEIPDWLREMSPEPTAEASDQTESDLG
                     GALFEFEEEKAEITEPTEWISGLETPEKIDLETSMLFSPQEIATEPEAELTLEEISEG
                     EPESFDISDTQPTRIQLQQEEAEILSEESAIPALDTLDTEIPEWLRGVGEEQMAAKEM
                     PSEPPAEELPAEITDFEQALEQVAAEEALSESPVEELPAEITDFEQVLEPSAAAEMTP
                     DISSESGVPLDDEDAFAWLESLAARQGAQEALLLEPEDRLEAPPDWVQKAAEEEQLAG
                     GQPGLSETSPSLEDVSEEEPLPDAQFEEATTTETSAPESLPIMEEILDEVVSPPETAQ
                     EEPIIESLEPMPEEAESLEARPDLPSWLTDVEMTKTPEAEAGWAPPVEKQPESVIEEE
                     TPVFAEQSAETLPETVNLNEAGQAELEQLPGVGFTRAQAIINYRQEHGPFSNVEELIN
                     VYGFGEVLVNSLIDKIKVVEAQPAEVLPEAVGDNQVILIQARNALIQGNISEALVHYT
                     HLVKEQKFLPEVIQDLNEALYRFPVDVSIWEALGEAHLRAGRLQEALDALTKAEELIR
                     "
     gene            12474..12923
                     /gene="ndk"
                     /locus_tag="AC16CB_15145"
     CDS             12474..12923
                     /gene="ndk"
                     /locus_tag="AC16CB_15145"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HIR3343857.1"
                     /translation="MEKTLVLVKPDGVQRGLIGEVFSRLERRGLRLVGAKFMAVSQEL
                     AETHYAIHKGKPFYEGLIRYITSAPVMAMVWEGPNAIAAVRQTMGSTRPTEAAPGSLR
                     HDFALEVGRNLTHASDTPENGEIEVALWFKPDELVSWERATDRWIFE"
     gene            12950..>13720
                     /locus_tag="AC16CB_15150"
     CDS             12950..>13720
                     /locus_tag="AC16CB_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR3343858.1"
                     /translation="MHTPQQIQSQLEAILPNVQKPGRYTGGELNQVLKEWDSVQTRLA
                     LVFPDIYDIGMSNLGLAILYDLINQRSDALVERAYNPWSDMESALRHAAIPLYSLESK
                     HSLADFDIVAFSLPYETLYTNALNALDLAGIPLFSTERDETHPLVIAGGHACYNPEPM
                     HLFIDAFVVGEGEDVIQEIIDVHQHWRQSRRGRNKLLKSLATIWGVYVPSLYQAEYNP
                     DGTFSHIEKLDEEAPLPVTKRIVAKLPPPTTNFIVPYID"
BASE COUNT         3557 a         3647 c         3252 g         3264 t
ORIGIN      
        1 tatttcacca cgtttgccat tgccaacccg ctcatctttc gttggtacct caccccaccg
       61 ttacccgctt actttctaat aatcctggta ggtattgaac gaatcaccca ccagatattt
      121 aactcccgaa aatctcgaat ttcttttccg aacatttcaa aaacacgtat aggaaatatc
      181 ctttctatcg ctttcgtggt aattgcccca tattttttga ccttgagagg atggacgttt
      241 catcccgatc atggtccaac tcgtcctgcg ccagagatgg cttacataca acttgaactt
      301 ttctaccacc aagctgctga atccctcaat gcagatatat cttcgcattc tactccacct
      361 ttattggccg cgggggatgt gggtatattg ggttattaca ccggtgcgcg catccttgat
      421 acggtcgggt tgaactcacc cgtctccacc agctacttcc cgctcgaccc agctttatat
      481 gtaatcaact acgctatccc gccagaccta atcctagacc aacgcccaga ttacgtggtc
      541 atcttggaag tgtatggtcg agaaggctta cttaaagacc cgcgcttcca ggaagcatat
      601 catcttcgcc agaaaattct caccgatatg tatgggagcg atgggatgct catctacgaa
      661 agaaagtaaa acagggctat gggggaatta agtccacata gccctgtctg aataaataac
      721 ccgtattgtc agctcagata ttcgcgcaat ttccgacgaa tagccgggtg tcgcaagcga
      781 ctaagcgctt gagcttcaat ctggcgcaca cgctcgcgag taacgcccat cttacgaccc
      841 acctcttcta aagtatatgc ttggccgtca aggagaccat agcgcagttg aaggattcgc
      901 acttcgcgcg gaggcaagcc attgagcaca ttatctaaat gttcacgcaa taggttatag
      961 gtagccgttt cgtcaggggc agccacttcc tcatcctgga tgaaatcgcc caggaccgaa
     1021 tcctcttcat cgtcggtggg tgtttcgagc gaaagtgggc gccgcgccac ctggatcata
     1081 ttttcaacct tctggggtgt gacgtctaat gctccagcca gctcctccac tgttggatcc
     1141 cgaccaagcc gttgggtcaa ctggtgctgt acgcgcagca gcttgttgat ttgatctccc
     1201 atgtgaacag gaacacggat tgtgcgaccc tggtcggcaa tggcgcgcgt cacagcctga
     1261 cgtatccacc aggttgcata ggtactgaat ttatgtccgc gacgataatc gaactttttt
     1321 gccgcgcgta tcagaccaat atttccttct tggatcaaat ctagaaaagg cacaccacgg
     1381 cccatatact ttttagcaac actgattacc agtcttgaat ttgcggtaat caggtgttcg
     1441 cgtgctgccc atccatcctc aatcaacaat tgcaactcaa ctcgccggcg tgagctaaca
     1501 ttacccctcg ccagttcctc acgtgccaag cgtcccgctt cgatgcgttg agccagctca
     1561 acctcttccc gagcggtcag caagggtaca cgcccgacct cttttaagta cagtccaatc
     1621 gtatcatctg tgtcgatatt tgctagataa ttgtcatcga tggaaagatc gcgctggctc
     1681 tcttcctcga tttcttcctc cgccaattca tcatcggatg gaccagctgc cacaggctca
     1741 tccatatatg ctatcccggc gctgatcaat gccgcaaatg cctcttccag ctggtcgaca
     1801 tcggtttccg catcaggaaa aaaactcaat atatcgtcaa tcgtgacata tgatttttgc
     1861 cgccccaatt cgatcaagcg cgcaatagca gggtgttctt cctctcgatc ttcaattgca
     1921 ctcaacaaat cttcgtcaat cattcagact ccattacatc ttcaaccaac ttaatatgag
     1981 aatacactct ttttaactca aaatcaatac ccaatttcca ctcactatgg cgtggttgtg
     2041 gttacagccg tcccagtaat aacctcttgg ggtgccaccg caccagtggg tgtcccagta
     2101 gggggcgcga tcggtggatt ttccagcatc tcgatcatgc catcaatatt aaatttactg
     2161 acgacataga ttgatccatt catacgtaca tagtacccgg attgggttgg tgtttcggaa
     2221 cccacatcca aaatatactt cttgccagct tcatcagtta tcgtcaaggt ataggctgga
     2281 ggtagcaaac caatctcatc ggcatctggt gcgggttgta aagtggtcat gatgttaatt
     2341 aagctcaatt gggtgactct cgcgtttaat tcttctagat caagattttg gcgttcgggt
     2401 tcggtcataa tccagagacc cccgcctagc tttctcaaaa acagacggtt attctggtca
     2461 tcgtctatct ttatatcgcg tatagtattc gcatcaatgt caagcagcct cgcaggaagc
     2521 gtcggtgtag gctcgcttgc agatttattc tcctccgagc gctgccataa caaagctgct
     2581 gccaaaatta cgataaacag ggctaaaagc acccaggttg gtcgccggat cataaatcat
     2641 tcctctacaa acatacaata tcggcgcgtc gatggttcac tcaagaatca acctctcttg
     2701 cgtctttgaa tccagacaac gataccggag aacaacacag cgccaggcac tagaaaaaca
     2761 aaaccaaata ggatcagccc catcgtgtac cttcccggtg ggatcagtac ccgttgggtt
     2821 gaatccttag gtgtcaagtt gatcaggttc tcctgtccgg cagcccaatc gatcgagttg
     2881 atgagcaaat cgccattgcc atattgcata aaattcgcat cgttggcaaa gtctgcatct
     2941 ccgtagacga tcagcctatt ttgcttgcct aaactctcaa tgacaactgc aagcgaaact
     3001 gggccaagca tatcaacccc tggatccggc tgtagctcaa tctgctcccc tccttgcaga
     3061 gattgcaaat cggtttctgc ccacgcgttc tcagcagtca gtacaagctc ggttcggtta
     3121 aggtcattaa attctcctgt taccgagaca ctacgcgttg tcgggaagag cgatacgagg
     3181 ccctgcatct tttgggttat aggatggctg gcgtaagaat tagccaccgc tacaaaaggc
     3241 tgctgagagg taaaatcgac gactatatca tttcccagaa cgatgccaaa gctctcactc
     3301 aggtatgtcg ctaatgggtc agggcgatca gcaaagtcgg tcagcaccaa gggttcttcc
     3361 atcaccacca acccccctcc cgactccaca aaagccttga gcttctccac ttcttcattt
     3421 gcaaggggtt gctgcggtcc agcgatcacg atcacagtgg cgtcatcagg gatattagcg
     3481 gtgctcaaaa ggttgagctt ccctgtgacg taatttttac cctctagcac cagcttgacc
     3541 aaagaatatg cctcatcccc cacaccctca ggatccctct cgccatgccc ggtcaagaaa
     3601 tacactgacc gcgtttcgcc cgaaatcaag cgtaccaggg cagatgtcat ctccttttct
     3661 gagacaaatt ccagcgattc ttggcggtct cccatcttca tgacgatcat accgtcacgg
     3721 gtaatctttg catcttttgc agcgattgga tcagcttctg ggtcgataaa gcggtaatta
     3781 aacttgcctt tgccttcgaa cttgtacttc tccagcaagt tttcagcact atcactagaa
     3841 attctggctg taaaaaaccc cagcgcttcg actggttccg gcaaattagc gagggtgtct
     3901 agtgtttcag ttgccagagt attttgctta tcttccgtaa ggtcccagcg ctttgaattt
     3961 ttatatccaa ggtagttaac caccaccaga atgccaacaa aggcgactgc catcaccaga
     4021 gcattgctac catatcgagc ttggcgaccg gtaagcgctg tccgcaccct gcccggatct
     4081 aacagggcaa agatagctaa tccgagcagc acaactccca ggctgatctg cagatataga
     4141 ttccactcac gctggattac atataacgaa atcgatgtca gggctgcaag tatcgccaga
     4201 tacaacccta ctggagcaaa gcgacgccat tcgggtttca ttatcgccac ctcctagact
     4261 cgaccgaaac cgacccaaga aagagcgcca gagcagtcag agacaagtaa tagatgacat
     4321 cgctgacatc aatgatcccc tgataaaaag tgttatagaa atgatcactg aaatccagat
     4381 accgcagcag ttcattcgca gtagcgctgc ctgaggcctg cgagggaaga ctaatcagcc
     4441 acaagaggag ataaactccc acagtagcga aaaaaacagc gatctggttt gagaacagcg
     4501 atgatacagc cacaccaatc gctaccaggg aagccgtgaa tagtattaca cccaggtaac
     4561 cagtaatcaa tggcccctga tcaatgcccg ggtccaccat ttgattaagg aagattgggt
     4621 aaagccaggt tactgctaat atggtcagca ggaagaggaa ccctcctagc cacttgccca
     4681 ccaccaactc ccaatcgcgc actggggcag tcaataataa ctccatggta cccaaacgct
     4741 gctcttcagc cagcaagcgc atggtgatgg cgggggtagt gaacaacaaa agggtcatca
     4801 gggggcccaa cacgccttgg atatttgggg gaaattgttg aacaatcgcc ccggcaacat
     4861 tgacgtagaa tattatcccc aaaataagca acaaaaagaa agccaccatg tacgcaaccg
     4921 ggctagcgaa gtagagctta tattcccggt ttgcaatcgt ccaaacattt ctcatagaat
     4981 tactcctctt ccccttcttc tgcttgaaca aaatcatcgt cagcagccat actaggcggc
     5041 tccggctctt cgcgagttag ctgcaagaag atatcttcca agctcaagcc tatcgaccga
     5101 agttctaaca agtcataccc ttgattaact gcggttctgg ctacctccgg acgcacatcc
     5161 tgcccagggg ctgtctcgaa ctcaaggccg ccatctccag atgtaaaaat acgcaataca
     5221 cctgggattt ttgccagcaa ttcttctaat ccttccacat cgccccggac gcgcaagcta
     5281 acgcgcttgg cgcctgccag gcgggattgt aaattttcgg gggtatcttc tgcaacgatg
     5341 cgtcccttat tgataatcaa aacccggttg cagacctgct gggcttccga aaggatatgg
     5401 gtggacagca gtacagtgcg ctcccgaccc agttcgcgga tcatattgcg cacctcaaga
     5461 atttgtgcag ggtctaggcc aatggtaggc tcgtccagaa ttaatacctc gggctcgtgc
     5521 aacaaagcct gcgcaacccc aatacgctgc cgcatccctt tggatagatt ggcgatgtag
     5581 ccttctgccc gatcggtcat atgcaccaga tccaatacgt catatactcg atcctcggca
     5641 ttgttcaatt tgcgcaggtc ggccataaat ttaagataat cgaatacagt catttccgga
     5701 tacaagggga cagtctccgg aagatagccc acccttcggc gcacatcgag tgattgttca
     5761 acaacatcga agccagcgac ggttgctctt ccggatgttg gaggcatgta accggtcaag
     5821 atgcgcatcg tggtagtttt tccggcccca tttggaccta agaatccaag gatctcccca
     5881 ttctcggcgt ggaaattcag ggtatcaatc gcacgtcgcg aaccataatc cttggtcaac
     5941 ccttctacta gaatcattct caactccttg acaacaaaaa gcacagcaac cgtcacccgg
     6001 cggcagtgct caattcaatt atgtatctac caacgaattc tggaactagc tgctcccgat
     6061 aagttaactt gtgatttgtt atacaacaaa aaaaggagta aagtcaagag gttctgtgct
     6121 ttctaatcga agtttaatca tttaaaaaga attgagtttg aaatataaat tgacttttca
     6181 atacccattg ggtaaaatta cctaccgtac aatacccccg tttaggcaag cacacaatct
     6241 tctatttccc aggaggcaag aataggaatg aatatactag gactcgccag gcatggtctg
     6301 aacgacttcg agcagatcgc tatcattgct gtagtcatca cagctttcat cagtttgatc
     6361 tacgcttggt tgctgcgtgg caatgtgctc aacaaagata aagggacgga gaaaatgcag
     6421 gaggtatgga acgcaatccg tatcggcgca gatagttacc tcagccgcca gcttcgttct
     6481 attcttccat tgataggtgt tctcactgtt gcgctttttc tgagcgtcta tgtagtccct
     6541 cctagtcgag aagccatcaa tgagtttggc cctgaaaacg cccagatcat tgtagccatc
     6601 gggcgcaccg ttgccttcat catgggcgca tcgttctcac tgatcgtcgg ccaactcggt
     6661 atgcgcatgg cgatccaagc cagcgtgcga gccgcctccg cttcacgtcg agggttcaat
     6721 gaatcgctct caatcgctta ttatgctggg acaatcactg gcatgctgac ggatggttta
     6781 gggctcttcg gcggtacgat aattttcatc atctttggca ccgccgctcc cgatgcactg
     6841 ctgggttttg gcttcggtgg caccttgata gctctcttca tgcgtgtggg tggtgggatt
     6901 tacaccaaag ctgctgatgt aggcgcagat ttggtcggga aggttgagaa agatattccg
     6961 gaagacgacc cgcgtaacgc cgccgtagta gccgatcttg tcggtgataa cgtaggcgac
     7021 tgcgctggca tggcagcaga tatctttgaa agttacgaag tcaccattgt ctccggcttg
     7081 attttaggct tgaccttggt agccatcgat cctacccaca gcctgaaatg gatcgtttat
     7141 cccctcatca ttcgagccat cggtgtgatc agctcaatac tgggaacttt cactgtacca
     7201 atctgggagc gcttcccgat caaattcctg cgcgcccacg acgccgaaga ggcaatgttc
     7261 cgttcgtatg aggtctctag tgtcaataca atcatctggt ctttcctcct ggcatttttg
     7321 tacgccaacg attggaaact ggccatgctc acaacaattg gtgttgggct ggcggttgcc
     7381 ttcaacccac tgacatccta cttcacctcc acaaaaagcc caccagtaaa agagattgtc
     7441 agctcaacca gaaccggtcc cgctactacc attctctccg gcctttctgt cggtatggag
     7501 tccagtgtgt gggcattggt tgtcattgtg gttagcttca tcttcgcttt attgctctat
     7561 ggaggccagg gttccatata cgttctttat gccgtcgcca tgatcggtat cggtatgctc
     7621 agccacaccg gcaacaacgt cgcgatggac tcttacggtc cgatatccga taacgccaac
     7681 ggcatcggtg aaatggcctg gcatgaccta gaagatgaat ccaccatgaa agctcgccag
     7741 atcatggctg atttggacgc tgtagggaac actaccaagg caattaccaa gggaatcgcc
     7801 atcgcatcgg ctgtcatcgc agcagttagc ctgtttgctt cttatattac agatgtgggc
     7861 cgggttcagg aacagttggg ggtacctatg atggaatcga tccgcgtatc tgatacgcgc
     7921 gtcttcatcg gtttgctgct aggtggcgcg ttaccctggc tattcagctc actttctatc
     7981 cgcgctgtca gtcgtgcagc tgggttgatc gtccttgagg ttcgccggca attccgtatc
     8041 cctggaatca tggaaggcaa agtaaagccg gattacgccc gggtagttga gatctcgact
     8101 gcagccgccc aaaaagaatt gataccattg gcggtcatct cagtgatgct ccctctggca
     8161 gtcggcttag tattgcaggt tgaagccctg ggaggcttcc tggcaggcat catcctgagc
     8221 ggccagcttc tggcagtctt catgtcgaat gcaggcggtg cctgggacaa cgccaagaaa
     8281 tccattgaag atgaagatcg cgatctagaa gccaacacag gtaagggctc cgaacggcat
     8341 aaagcaggtg tcgtcggcga taccgttggc gacccactca aggatactgc cggcccggct
     8401 ctcaatccca tgatcaaggt aaccaacctg gttagcctga ttgcggctcc tatcattgtc
     8461 aaatacagct cacccaatgc aatcgttatc gttgtggtcg tgctgctgct ggcatcaatt
     8521 gtatgggcga ttaaccaaag caagaaaccc gctgaagaaa tctcgcagga ggtcgttcca
     8581 ggcgattagc cttgttattt tttggctttt cagcatgaac tacggagtgt tgcggacggt
     8641 ttaagcccgc aacgctccat ttttgttagt tttacttgca actttgaccg atttggggca
     8701 tacttaaaat cagctatgat tggtagagaa gcaatactat taaaaagtag acctaatttc
     8761 cgtcaccctt aggcgaggga acctgattaa tggccaaaat acctttacgt gcttataacc
     8821 gcgagatcga gaagttgatt gatcaaaatc aactcgaaca agcaattgct cacgcgcgcc
     8881 atatcctgaa ttatttccca aaacatgtca attcataccg actactgggg aaagcctacc
     8941 tggaaagcca gcgctatggc gatgcagccg atgtctttca gcgcgtgctc tcctcaatcc
     9001 ccgatgactt catatcccat gtgggtatga gcataatccg cgaggatgaa gcaaatctgg
     9061 atcatgccat ctggcatatg gaacgcgcct tcgaagttca gccagccaac ggcgctatcc
     9121 agggtgaatt gcgccggttg tatggtcgcc gagatggaat ggaaccacca aaggtccggc
     9181 ttacacgcag cgcactggca cgtatgtaca ttaagggtga gctatttcaa caagccatca
     9241 gtgaggttcg tgctgcactg gcggaagatc cacaacgccc agatcttctt tctctactgg
     9301 cgcaagctta tttccagaat ggacagcgtg ttgaggccgc aaacacctgt agcagtctcc
     9361 tcaacaaatt tccttacagc ctggaagcaa accgcatctt agcagaaatc ctcagcagca
     9421 cggagcgcgc tgaagaggcg caagtctacc gccagcgcgt ccaggctctt gagccctatt
     9481 ttgggcatac ctctcccgct tcacctacac cagatctggt gtctgagaca gccgtggtgc
     9541 ttgaaaagct ggtatggagc cctggtcaat cagttacagg cgcacaaaaa caaccagaat
     9601 gggccgcctc cttgggtgtc gagctcgaag ccactgcacc ggcggaagaa gggccaccag
     9661 agtggcttgc agatcaacct gaagcagagc catcagcgac acccgaggca catgaggagg
     9721 gggatgaagc tccttcagag acgcctaccc caccatccga aaccctattt gctgaattat
     9781 ccgaggatgc aatccccgac tggatgaagg aagcggggtg gcaatctgcg tccggtgatg
     9841 tagaagagcc tgttgatatt ctggaggggc ctgcacaaga cgagttaacc cctgctcaaa
     9901 tcccagattg gctacaatcc attgctccag gggagaaacc cgaagctgaa atcagtgacg
     9961 aaacgggtga gtcaatgcca ccgattgaag aaaccttgag tgacagcgac gctctctcct
    10021 ggctcgatga tgcaccccct ggtccttcgg ataccgtcgc ttcctggtta gatgaaaacc
    10081 aacccgccgt acctgatata tctgagaccg aatcagagac ggaagaaaaa accgaagttc
    10141 cggcatggct gaaggatttg caacaacccg aaccaccttc ggtgtcagac gaaacgctca
    10201 caggcaccct gacatcacct gacgaagtta gcgaattgga accccaagca caaagccttt
    10261 tacctgagat tgaggctgag agtgatttcg ctccatcaac aactgaggcc gatgagggcg
    10321 agaaaattcc agattggtta caggaattgg gtggggcacc gagtgttaag gaggaaatcg
    10381 aaccaactga agagcaacct gattggcttc gccaatccga tcaggaagaa tggcaaacta
    10441 gcgaaccatc ggagacagca acgccagaaa cgctttcaat gccacctgta gaggaattgc
    10501 ccatttcatc cgaagacgat gaacttgctt ggcttgatga cctagcggac aagcaaggca
    10561 aaaaggaatc tataccactg accccagaag atgcgctcgc tgaagctcct gattgggtgt
    10621 tggaagcagc cctagaaacc ccagaaatat tgcccactgc cgaggatgcc ccaaccagcc
    10681 ctctcagtga gcttccaact gaggtcgcag atcaagatac cgaagattta caattaccgg
    10741 atcaagtcga gccaatcgag atacctgatt ggctacgcga aatgagccca gagcccacag
    10801 cagaggcaag cgatcaaacg gaatctgacc tcggtggtgc attgtttgaa ttcgaagaag
    10861 agaaagcaga gatcaccgaa ccaacagaat ggatatccgg actagagact cctgagaaga
    10921 tagatttgga aacttcaatg ctcttcagtc cacaggagat cgctaccgaa cccgaagcag
    10981 aactcaccct tgaagaaatc tcagaaggtg agccagaatc ctttgacatc agcgataccc
    11041 aaccgacacg catccaactt cagcaagaag aagctgaaat attgtccgaa gaatcagcca
    11101 ttcccgcatt agataccctg gacaccgaaa tccccgaatg gctacgcggg gtgggcgagg
    11161 agcagatggc tgctaaggag atgccatcag agccgccagc cgaggagcta ccagctgaga
    11221 tcactgattt cgaacaagct ctggagcagg tagctgctga ggaggcattg tcggagtcgc
    11281 cagtcgagga gctaccagcc gagatcactg atttcgaaca agttctggag ccaagtgctg
    11341 ccgctgaaat gaccccggac atatcatctg aaagcggggt tccactagat gatgaagacg
    11401 ccttcgcctg gctggaaagt ctggctgccc gccagggcgc ccaggaagct ctcctcttgg
    11461 agcccgaaga tcgcctggaa gcacctcccg actgggttca aaaggctgct gaagaagagc
    11521 aattggcagg tggtcaacct ggactaagcg aaactagccc ctcattggag gatgtctcgg
    11581 aagaagaacc cctgcccgat gctcaatttg aggaagccac gacgaccgaa accagcgccc
    11641 cggaaagcct ccctataatg gaagaaatcc tggatgaagt cgtttcgccg ccagaaactg
    11701 ctcaagagga gccaatcatc gagtcacttg agcctatgcc agaggaagcc gaatcgctcg
    11761 aagcccgacc tgatctcccc agctggttga ccgacgttga gatgaccaag acacccgagg
    11821 cggaggcagg ctgggcaccc cctgtagaaa aacaaccgga atcagtaatc gaggaagaaa
    11881 cccctgtttt tgcggaacag tctgctgaaa cattaccaga gacggtaaat ctcaatgaag
    11941 ccggacaagc agagctcgag cagttgcccg gcgtgggatt cacccgagca caggcgatta
    12001 tcaactaccg ccaagagcat ggcccgttca gcaatgtaga agagctgatc aatgtctacg
    12061 gatttggtga agttctggtc aattcgctta tagacaaaat taaggttgtc gaagcccagc
    12121 cggctgaagt tttgcctgaa gcagttggtg acaaccaggt aatcttaatt caggcacgca
    12181 atgcccttat tcaaggtaat atctcggagg ctttggtaca ctatactcac cttgtcaagg
    12241 aacaaaaatt cttgcctgaa gtcatccagg atctgaatga agcactttac cggttcccag
    12301 tcgatgtctc catttgggaa gcccttgggg aagctcatct gcgcgctgga cggcttcaag
    12361 aagcactgga cgcactcaca aaagctgagg aattgattag atagacattg aaggtccaat
    12421 gcagtttgaa gaaaggcatc ccaagctgca gtatttttaa tttggagaat tacttggaaa
    12481 aaacacttgt ccttgttaaa cctgatggtg tgcagcgtgg attgatcggc gaggtgtttt
    12541 cacgtcttga acgacgaggc cttcgcctgg tgggagcgaa atttatggca gttagccagg
    12601 agttagccga gacacactac gcaatccata aaggtaaacc tttttatgaa ggtctaatac
    12661 gctacatcac ctccgcgcct gtgatggcaa tggtttggga aggtcccaac gccatcgccg
    12721 ctgtgcgcca gacgatgggt tccacgcgtc ccaccgaggc cgcacctggt tcactacgac
    12781 acgatttcgc cctggaagtt gggcgcaatt taactcacgc atcagataca cccgagaatg
    12841 gtgaaattga agttgcccta tggttcaaac cagacgagct cgtctcctgg gagcgcgcta
    12901 cagatcgctg gatattcgag taagtcttgt gtttttagga taattattgg tgcatactcc
    12961 acaacagata caatctcaac ttgaagctat tcttccaaac gttcagaaac ctggacggta
    13021 taccgggggc gagttaaatc aggttctaaa agaatgggat agcgtgcaaa cgcgcctggc
    13081 tctcgtattt cctgatatct acgatatcgg catgtcaaat ttgggattag ccattctata
    13141 cgatctcatc aatcagcgca gtgatgcgct ggtcgagcgc gcttataacc cctggagcga
    13201 catggaatcc gccttgcggc atgctgccat acctctttat tcgttggaga gcaagcattc
    13261 cttggcagat tttgatattg ttgccttctc gcttccctac gaaacactgt ataccaacgc
    13321 gctaaacgcc ctagatctgg caggaatccc tctattctcc accgaacggg atgaaacgca
    13381 cccccttgtg attgccggag gccatgcttg ttataaccct gaaccaatgc acttattcat
    13441 tgatgctttt gttgtcgggg aaggcgaaga tgtgatccag gagattatcg acgtgcacca
    13501 gcattggcgc cagagcaggc gcgggcggaa taaactgctt aaatctcttg caacaatatg
    13561 gggtgtgtac gtcccatcac tctaccaggc agaatacaat ccggatggca ctttttcaca
    13621 catcgaaaaa ctagacgaag aagccccctt gcccgtcacc aagcgcattg tagccaagct
    13681 gccaccacct accaccaatt tcatcgtgcc atacattgac
//
LOCUS       DBNGSX010000511         2052 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_883682, whole genome shotgun sequence.
ACCESSION   DBNGSX010000511 DBNGSX010000000
VERSION     DBNGSX010000511.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_883682"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="AC16CB_15155"
     CDS             <1..238
                     /locus_tag="AC16CB_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3343859.1"
                     /translation="GERLEEDFEGAGLRGHLAYRFVPEKGRTILIQQETVEPTGILWV
                     LEPIIKRVLLRQLWKRLQGIKQILEAGWVVADQK"
     gene            complement(285..1838)
                     /locus_tag="AC16CB_15160"
     CDS             complement(285..1838)
                     /locus_tag="AC16CB_15160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015158548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3343860.1"
                     /translation="MSDSIIIIGAGIAGLSAGCYGQMNGYRTQIFELHDIPGGLCTAW
                     ERKGYIFEGCIHYLFGSGQDQPFNHIWQELGAVQGRQMIHHDEFIRIKDNDGKTLIVY
                     ADPDRLEEHLLELSPADRDLIKDFTKGVRQFTSFDMSVLQEKPRDLFGPTDGLNLGRR
                     MLPFIQPLMKWGNISAQDFANRFRDPFLRRAIPQMFAWPEIPVMAGLSLLAYMHTKNA
                     GFPIGGSLPFAQTVEQRYIELSGKVHYKAQVEKILTENGRAVGVRLYDDSVHYADTVI
                     SAADGRGTVFDLLDEDFTDAKINKTYDGHLPIISQVQVSLGVSRDLSAEPHWVVYLLD
                     EPVIIAGEERHELSVKHYCFDPSLAPTGKSVIEVMLPSQYGYWQRIYGRKLYDTEQLQ
                     VADQVIELLENIYPDLSQSIEVTDVATPLSYERYTGNWQGSTCGWLLTKETMRMMILG
                     IKKTLPGLDRFYQVGQWVEPGGSLPLVAMSGRNAIQLICHRDGKPFVTSLPASQAPRT
                     DFRLGSERK"
BASE COUNT          504 a          508 c          548 g          492 t
ORIGIN      
        1 aggagagcgt ttggaggaag atttcgaagg cgcgggattg cgaggtcacc tagcctatcg
       61 gttcgtgcct gagaaggggc ggacgatact gattcagcaa gagaccgtag agccgacagg
      121 gattctgtgg gtgctggagc cgatcatcaa gcgtgtactc ttacgccagc tgtggaagag
      181 gcttcagggc ataaagcaaa tcttggaggc cggctgggtg gtcgctgacc agaaatgagg
      241 gtgtgaggcg ttcacatact cattccccct cggtttggct gacctcactt ccgctccgag
      301 ccgagacgaa aatctgtcct cggggcttgg ctggcgggca ggctggtcac gaaaggttta
      361 ccatcgcgat ggcagatcaa ctggatggca ttgcgacctg acattgcgac cagcggcagg
      421 ctgccacccg gctcaaccca ttgacccact tgataaaaac gatccaggcc gggcaaggtc
      481 tttttgatgc ccaggatcat catgcgcata gtctctttgg tcagcaacca tccacaggtc
      541 gaaccttgcc agttgccggt gtagcgttcg taactcaagg gagtggccac gtcggtgacc
      601 tcaattgact gactgaggtc tggatagata ttctccaata actcgatgac ctggtcagcc
      661 acctggagtt gctcggtatc gtacagcttt cgcccgtaaa tacgctgcca gtagccatac
      721 tgggagggta acatcacttc gatgacggat ttccctgtag gagctaagct ggggtcaaag
      781 caatagtgct tcacactcaa ctcgtggcgt tcttctccag caataatcac cggctcgtcc
      841 agcaggtaaa ccacccagtg aggttcagca gatagatcgc gagatacccc tagtgaaacc
      901 tggacctggg agattatcgg cagatgaccg tcataggttt tgttgatttt agcgtcggta
      961 aaatcttcat ctaaaaggtc gaaaacggtg ccacgcccat ctgcggctga tatgaccgta
     1021 tctgcgtaat ggactgaatc atcgtataag cgcacaccta cggcgcgccc gttttcggta
     1081 aggatctttt ccacctgggc tttgtaatga acctttccac tcagctctat atagcgttgc
     1141 tcgaccgtct gtgcaaacgg caatgatcca ccaatgggaa agccggcatt ttttgtatgc
     1201 atataagcca ggagcgaaag accagccatc acaggaattt ctggccaggc gaacatttga
     1261 ggaattgccc tgcgaaggaa ggggtcacga aagcgattgg caaaatcttg ggcagagata
     1321 ttgccccact tcatcaacgg ctggataaat ggcagcatgc ggcggcctaa attgaggcca
     1381 tccgttggac caaaaagatc gcgaggcttt tcttgcaaaa cagacatatc gaaggaggta
     1441 aattgccgca cccccttggt gaaatccttg atcaggtcac gatctgcagg cgatagctcc
     1501 aacagatgct cttccaggcg atccgggtcg gcgtaaacaa tcaaagtttt tccatcgtta
     1561 tccttgatac gtataaactc gtcgtggtga atcatctgtc ggccttgaac ggcacccagc
     1621 tcttgccaga tatggttaaa tggttgatct tgcccggatc caaacaggta gtgaatgcat
     1681 ccttcgaaga tataaccctt acgttcccag gcggtgcata atcctccggg tatatcgtgc
     1741 agttcgaaga tctgcgtgcg gtaaccgttc atctgaccgt agcacccagc ggatagacca
     1801 gcaattcccg ccccaatgat aatgatcgaa tcggacatgt aagaccctcc catgcgtgtc
     1861 ggtgtagcat atttcggcag gattattgta ctacgttgct tttaagcttg gcaggaataa
     1921 agcctattta aatgttggat ggggaagggg tatgtagaat cccgcctatc gatgaccttt
     1981 tttcgaatgg ataaatcccg ttgttcacct ggagagtggg tttacccgat ttagaagaat
     2041 gtgactatcg ga
//
LOCUS       DBNGSX010000512         6015 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1644109, whole genome shotgun sequence.
ACCESSION   DBNGSX010000512 DBNGSX010000000
VERSION     DBNGSX010000512.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6015)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1644109"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="AC16CB_15165"
     CDS             complement(<1..193)
                     /locus_tag="AC16CB_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343861.1"
                     /translation="MKFSYLVSLPERILRASAASLGGFIYEASLLVLPGWLRETRLYT
                     AIVAGLLRIVIELVGGARGI"
     gene            complement(501..2243)
                     /locus_tag="AC16CB_15170"
     CDS             complement(501..2243)
                     /locus_tag="AC16CB_15170"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR3343862.1"
                     /translation="MHDASDQIQDLVALNQIIETLNRTVDMQSALDNTLARLLEVMGM
                     ETGWIFLRDPASQNLWAGRGYALASHKNLPPALALDNPEAWEKGCDCEGFCDKGKLVG
                     AYNEVQCSRLVEASGERGGLLVHASSPLRSGEEVLGILNIAAADWSSFTPRLLAFLTN
                     VGAHMGAALERARLYNLLRERRIDEQRTLISFTNQLLKRLELDELLNYLALEVPRLLE
                     AEGCAIVLHAHQPGELPTVFLHQWPEPNIPAMSAQEVLKYWQAQAPCCLATAPLVVES
                     GELGWLMLHSPEDKQLNDDETRLLQLLANQVALAIEKSRLHQVELSRQRLEVELETGM
                     RLQRSLLPKRLPDLPGWEFAAYYQPAQSLGGDFYDFFTLPGDDQRLGMVIADVMDKGL
                     PAALFMTLCRSIFRTTSISDISPAESLQRANLLIRRDIGCDDEYTDDIGYFVTAVYAT
                     LEIDSGRVNFANAGHNRPLCLRANGRVDELSSQGIVLGLFNDIFLEESQITLAPGDSL
                     MFYTDGTTEAINEQGELFNTSRLMNLLEHSDGLSAKVILDRVVATVSDWLGTQPQSDD
                     LALFIIRRSPSNDI"
     gene            complement(3241..3903)
                     /locus_tag="AC16CB_15175"
     CDS             complement(3241..3903)
                     /locus_tag="AC16CB_15175"
                     /inference="COORDINATES: protein motif:HMM:NF015722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HIR3343863.1"
                     /translation="MVRVPAGEFLYGDSQQKLSLPEFWIDKTPVTNREFANFVKATGY
                     KTTAEQTGIGCAHTSGKWEDIDGVDWQHPNGPQTDIQSKADHPVVQVSWDDAVAYATW
                     AGKRLPTEQEWEKAARGTDGREYPWGNQEPTRDLCNLNMNEKGTTPVGKYSPQGNSPY
                     GCVDMAGNVWEWTASDHESDSKILRGGGWSHPAVHVRTTDRSPHHPDDRYDTDGFRCV
                     VG"
     gene            4114..>6015
                     /locus_tag="AC16CB_15180"
     CDS             4114..>6015
                     /locus_tag="AC16CB_15180"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3343864.1"
                     /translation="MNCPNCLSENPATAKFCMNCGSPLAVESAEVGKQSQFKLDRYLP
                     QELITKLEAARSRNSMVGERRVITMLFCDVKGSTAAAEKVDPETWTEIMNGVFEFMIR
                     PIYKYEGLVPRLMGDAILAFFGAPITHEDDPQRAVLAGLEIQEGIKSYAREIRLKHGL
                     EFGLRVGINTGLVVVGEIGSDLRMEYTAIGDAINLAARMEQTAQVGTIQISDETYKLV
                     APFFEVEPLGEVEVKGKSAPIKTYRVLGVKSTPGHLRGLEGFTSPLVGRESQLGLLKA
                     RLEDLRIGRGSVVAVMGEAGLGKTTLINQVKESHKDSSSIWLSGDSLSYTRSISYYPW
                     RQIIRQSVDAHEDDSPAEVRHKLGYVCECCTLPGGDIPFLEAMLAIESEESLKVVMGF
                     QGDALNQRMVDATRGFLCGLAAENPLAIVFDDLHWSDEASLSLLLNVVDLSTSQPILF
                     ICMSRTDRNVPSWNSFQKMSEKLANGFHSIDLAPLQVNQTETLLNNLLGVKNLPKSIR
                     DLIVDKAQGNPFFVEEIIRSLIETKQIIRQNSQWEAVSDNAKITLPNTLRGVLNARID
                     RLPETSKHVLQNAAVIGRLFDLRVLRPLTSLNGGLDTHIQFLKEASLIEALHNEYAFR
                     HVLIQEAAYE"
BASE COUNT         1449 a         1465 c         1567 g         1534 t
ORIGIN      
        1 ggataccccg ggctccgccg accagctcga tgacaatacg taacaatccg gcaacgatgg
       61 cagtgtaaag gcgcgtttca cgaagccatc ctggcagcac cagcaagctc gcttcataga
      121 tgaatccacc gaggctagcg gctgaggctc gcaggatacg ttcaggcaat gaaaccaggt
      181 aagaaaattt catgcgaatt gaggcaggct atcaatgttc catgaccttg gtgtttttcc
      241 tgattttgct atctttgcat tttcctcttt gacaatttac ataacgcaaa gaacgcctct
      301 tgccatgaga ggttattaag atcgttggca ttgagagtgg tcgatgcagt aattgtcaca
      361 gcacgcaacc gggtaactat cttggacata gatggacttg ctgttcagat ctttccaggt
      421 atcacctaac aagcgaaaaa gcgccaggaa cagattttga atacgatgca atggcgattg
      481 aaacgagttt tgcccaacat ttatatatca ttggatggcg aacgtcgtat gataaatagg
      541 gccagatcgt cggattgggg ctgcgtgcca agccaatcgg atacggtggc gactactcgg
      601 tccaggatca ccttagcgct gagtccatca ctgtgctcaa gtaggttcat caagcgtgag
      661 gtgttgaaaa gctcgccttg ttcattgatc gcttcggttg taccatcggt atagaacata
      721 aggctatcgc cgggtgctag ggtgatctgg ctctcctcta aaaagatatc gttgaaaagt
      781 cctaggacga tgccttgact ggagagctca tctacccgac cattggctcg taggcaaagc
      841 ggtcgattgt gcccggcatt agcaaaattc accctaccgg agtcgatctc aagggtagca
      901 tatactgcgg tgacgaaata gcctatatcg tccgtgtatt catcgtcgca gccaatatca
      961 cgacggatca gcaggttggc gcgctgtagt gattcagcag gagatatatc actgatggag
     1021 gtggtgcgaa agatcgagcg gcagagggtc atgaacaacg ccgccggaag acccttatcc
     1081 atcacatcgg cgatcaccat ccccagtcgt tgatcgtcac ccggcagagt gaaaaaatcg
     1141 taaaaatccc cccccaaaga ttgcgctggc tggtaataag cagcgaattc ccagccaggc
     1201 aagtccggca ggcgctttgg cagcagactg cgttgcaagc gcattccggt ttcaagctcc
     1261 acttctaagc gctggcggga gagctctacc tggtgcaggc gggatttctc gatcgccagg
     1321 gcgacctggt tggcaagaag ctgaagtaag cgggtttcgt cgtcgttgag ctgcttatcc
     1381 tctgggctgt ggagcataag ccagcctagc tcgccgcttt caaccaccag cggcgcggtc
     1441 gccaaacaac atggagcttg ggcctgccag tacttgagca cctcctgggc tgacatcgcc
     1501 gggatgttgg gttcaggcca ttgatgcaag aagacggttg ggagttctcc cggctgatga
     1561 gcgtgcaaaa caatggcaca tccctcggcc tccagcaagc gtggcacttc caatgccaga
     1621 taattgagca gttcgtccag ctccaaccgt ttcagaagtt ggttggtaaa actgatcagt
     1681 gtgcgttgct cgtcaatacg acgctcacgc aataggttgt acagtcgggc gcgctccaga
     1741 gccgctccca tgtgggcgcc cacattagtc aagaaagcga gtaggcgagg agtgaaagat
     1801 gaccaatcgg cagcagcgat gttcaagatg ccgagcacct cttcaccgga gcgaagtggg
     1861 gaagaggcgt gcaccaaaag gccaccgcgc tcgccactcg cttcaaccaa ccgactacat
     1921 tggacttcgt tataagcccc aaccagcttg cctttgtcgc agaatccctc gcaatcacag
     1981 cccttctccc aggcttccgg gttatccaaa gccagtgcgg gtggcaggtt cttgtgagac
     2041 gccaatgcat aaccccgtcc ggcccacaag ttttggctgg ctgggtcgcg caggaaaatc
     2101 cagccggtct ccatgcccat cacttccagc aggcgcgcca gggtgttatc caatgcactc
     2161 tgcatatcca cggtgcgatt gagcgtttcg atgatctggt tgagtgcgac cagatcctgg
     2221 atttgatctg aggcatcatg catggtttac cctccgcctt aggtagctaa gatcttgtag
     2281 taacgcagta cagtatggaa ttgcctatcg tttattactt atcgcaatct atcgcagttt
     2341 cttccttttc cgattcctga ctaactgtat gtgtacttat gctcacaggg aagcagattg
     2401 aaaattctaa aaatccatcc attgtgtgtg catgatcttc tttaataata tagacatact
     2461 taaagaataa ctctccagag atgcaagata ctgctgtaat cttcatacat tatcggcaag
     2521 tttcccttta cgtcatcagt tatataattt tcattgacct cagaaacggc aggatattgg
     2581 tttaaagaat ttttcattgc ccacctgcac tatttctatt catacgaccc ttaacttgag
     2641 ctttgtcatg ttgattctcc cctccctaca caatttatgt ggggaagtgg attctattct
     2701 tattgttgta gatccatgat gtcgggtggc ggttaacatt ttttgggatt cttgtggggg
     2761 taggttgaaa tgggcttcga attggattcc tcaatatttc actctgatgt gctacgctgc
     2821 attgaaaact gttttgcctg atgataattg ttgatattag tatacagaca ttctcttctc
     2881 gattcaatag ggataacaca ttttgagaga actcacgccc aatttccaac cgtgtggtag
     2941 tcatttggtt gcgtcagggg tcgcacgacc caaaagtgaa catcaggagt gtaacctcaa
     3001 cggaatcgag atgcgccgcc cattgccacg gcgagagtag gcaattggct ccatgtgccg
     3061 cttagtcctg ttcccagtgc aaattgagag ccttgcccca gaaatggagg tcttcgaaag
     3121 gttatatcat cttcttggga aaggtacact gggtgtgaga atcgtgcaca ggatggtgct
     3181 cccatgcatt gtgaggggga ggctgttttt ctgggaacgg ctaactcagt ctcgcggaaa
     3241 ctagccaacc acacaccgaa agccatcggt atcataacgg tcgtctggat ggtgggggga
     3301 gcgatccgtc gtgcggacat ggacagcagg atgcgaccag ccgcccccac gcagtatctt
     3361 gctatcgctt tcatgatcgc tggctgtcca ctcccataca ttcccagcca tatccacaca
     3421 tccataaggg ctattaccct gcggcgaata tttgcccact ggcgttgtcc ctttttcgtt
     3481 catgttgaga ttgcataaat cgcgcgttgg ctcttgattt ccccatgggt actctcgccc
     3541 atccgttcct cgagccgcct tttcccattc ctgctccgtc ggcagccgtt tgcctgccca
     3601 tgtggcataa gccaccgcat catcccaact cacttgcaca actggatgat ctgctttgct
     3661 ctggatatct gtctggggcc cgttagggtg ttgccagtcc acgccatcga tgtcttccca
     3721 cttgccgctg gtatgggcac accctatccc tgtttgctcg gcggtggtct tgtatccagt
     3781 ggctttcaca aagttggcaa attcccggtt ggtgacgggt gtcttgtcta tccagaattc
     3841 gggcaagctc agcttttgct ggctgtcgcc gtataagaat tcgcccgctg gtacgcggac
     3901 catctctttg ccgtctgtct cccaaagccg gatgttttgc atcaacagtt attttagcat
     3961 gaacacaaaa acagattgac ttattagggc aatcccattt gggcgatttc ccaccattga
     4021 ggagagaaat tcagatgttt tggttggggt atgccctgaa ttaggggatg aaatatgcta
     4081 aaattcgtca gaaatataga attattggtt aatatgaact gcccaaattg cctgtcagaa
     4141 aatcctgcaa ccgcaaaatt ttgcatgaat tgtggtagtc ctctggctgt tgaaagtgcg
     4201 gaggtgggga agcaatcaca attcaagctg gatcgttatc ttccacagga actgatcaca
     4261 aaacttgaag ctgcacgctc acgcaattcg atggtggggg aacgccgtgt cattacgatg
     4321 ttgttctgcg atgtaaaagg ttcgaccgcc gcagctgaaa aggttgaccc cgaaacctgg
     4381 accgaaatca tgaacggtgt gttcgagttt atgattcgcc cgatctataa gtatgaaggg
     4441 cttgtgccgc gtctgatggg cgatgccatc cttgcatttt ttggcgcccc gatcacccat
     4501 gaggacgatc cgcaacgcgc ggtattggcc ggattggaaa ttcaagaggg tatcaaatca
     4561 tacgcaaggg agatccgcct caagcatgga cttgaatttg gtctgcgggt tggaataaat
     4621 accggtctgg tcgtggttgg tgaaattggt tcggatttac gcatggaata caccgccatt
     4681 ggcgatgcga tcaatctagc tgcccggatg gagcagaccg cacaggttgg aacaatacaa
     4741 atttcggacg aaacgtataa actggttgcg cctttctttg aagttgaacc actgggagag
     4801 gtggaggtca aaggcaaatc tgcgccgata aaaacttatc gagtgctggg agtgaaatcc
     4861 acgccaggtc acttgcgcgg actagaaggg tttacttcgc cgttggtggg aagagaatcg
     4921 cagttgggat tgttgaaggc gagattggag gatttgagaa tcgggcgggg atcagtcgtc
     4981 gctgtgatgg gggaagcggg acttggaaag accacgttga ttaatcaagt gaaggagtca
     5041 cataaagatt cgagttctat ctggctgagc ggcgactctt tatcctacac gcggtcgatc
     5101 agttactatc catggaggca gattatccgt caatcggttg atgcccacga agatgactcg
     5161 cctgctgaag tccgccataa gttgggatat gtgtgtgagt gttgtacatt gcctggcggt
     5221 gacattccgt ttctggaggc gatgctcgcc atcgaaagcg aggagagttt aaaggtcgtc
     5281 atgggctttc agggggacgc gctcaatcaa cgcatggtgg atgccacacg tggttttctg
     5341 tgcggacttg cagccgaaaa tccactggcg atcgtgttcg atgacttgca ttggtcggat
     5401 gaagcctctt tgagtttatt gctcaacgtc gtggacttga gcacatctca accgattttg
     5461 ttcatctgca tgtcacgcac tgacaggaac gttccaagtt ggaattcgtt tcagaagatg
     5521 agtgagaagc tggcgaatgg gttccattcg attgacctgg ctcccttgca ggtgaatcaa
     5581 accgaaacct tattgaacaa tctgctcggc gtaaagaatt tgccaaaatc catccgcgat
     5641 ttgatcgtgg ataaagcaca gggcaatcca tttttcgtgg aagagatcat ccgctcgctc
     5701 attgagacga aacagatcat ccgccagaac agccaatggg aggccgtgag cgataatgca
     5761 aagatcactt tgcccaacac gttacgcggc gtgttaaacg cccgtattga ccgtctgccc
     5821 gaaacatcca aacacgtatt gcaaaacgcg gcggtgatcg gtcgtctctt cgatttacgt
     5881 gtcctgagac cgctgaccag cctcaatggt ggactcgaca cgcatatcca gtttttaaag
     5941 gaagccagcc tgatcgaggc gctccataac gaatatgcat ttcgtcatgt gttgatccag
     6001 gaagcggctt atgaa
//
LOCUS       DBNGSX010000513         3598 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_328191, whole genome shotgun sequence.
ACCESSION   DBNGSX010000513 DBNGSX010000000
VERSION     DBNGSX010000513.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_328191"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..451
                     /locus_tag="AC16CB_15185"
     CDS             53..451
                     /locus_tag="AC16CB_15185"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3343865.1"
                     /translation="MQILNASLEDLDQISELTRIVWHASFIDMISEDHIEYMLSLKYT
                     PEKLRADILDSGAIYLKLVERDQLVGFASFRSSDELNLLLLDKLYVHPAQQRKGNGTR
                     LMSQAKKRRSKKDIRESFSPLSLVPTGDTT"
     gene            583..1548
                     /gene="speB"
                     /locus_tag="AC16CB_15190"
     CDS             583..1548
                     /gene="speB"
                     /locus_tag="AC16CB_15190"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HIR3343866.1"
                     /translation="MSAKNPNPAFGAHLEPFHGITTFMRLPASRQLEGVDVAITGIPF
                     DSGTSYRSGTRFGPRKIRESSLLLWGFNTVLGVSPAETLKIVDYGDIEIIPVEISKTM
                     GNITQEISHLLQNGVTVIALGGDHSVSLPLLRAHAAQFGPLAVIHFDSHPDTWDQEFP
                     GQPYSHGTPFRRAIEEGLIDVNAYLQIGIRGSVGHANDLPEAREMGARIFTIDQVFEL
                     GIPSIIREMHKTVGDRKVYISLDIDSVDPAFAPGTGTPEVGGLSSYHILQLVRGLQGM
                     NFVGFDLVEVSPPYDHGDITSILAANLVFEFLSLLALRSRNRPGA"
     regulatory      1749..1830
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2260..2673
                     /locus_tag="AC16CB_15195"
     CDS             2260..2673
                     /locus_tag="AC16CB_15195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3343867.1"
                     /translation="MDLIIKAANATAGKILIVDDDPDTCQLLQFLFQRKGYQTKAVTS
                     GWQAIQHVEDQPPDLVVLDVLMPEMDGWETFRRVRELSNSLVLFLSALGGGEHAAQAL
                     QMGADDFIRKPYSNVELLARVEMALSRARGNTSRA"
     gene            2886..3263
                     /locus_tag="AC16CB_15200"
     CDS             2886..3263
                     /locus_tag="AC16CB_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343868.1"
                     /translation="MSLRNLFIASSIIGFIFGLGFYLAPAQTIGPYGVEPPTQSTLYF
                     IRFVGASFIGFSLLAWLVKDEPASDTRQKILLSYFVSYLLILIVMLIEQLTTPVGALV
                     WVNIVIQALFTAGFGYYRFIKTS"
BASE COUNT          850 a         1000 c          907 g          841 t
ORIGIN      
        1 ggatttggca gccaacggtt tgaggctgtc cgatattcgt tgccggtaat cgatgcaaat
       61 actgaatgct tcactggaag atctcgatca gatctccgag ttaacccgga ttgtatggca
      121 cgcaagcttc attgacatga tctccgagga tcacattgag tacatgctgt cgctgaaata
      181 taccccggag aagttgagag cagatattct agatagcggt gctatttatc tgaagcttgt
      241 tgaaagagac cagctggtag gctttgcatc gttcagatct tcagacgagc tgaatctcct
      301 tcttcttgac aagctttatg ttcaccccgc acaacaaagg aagggcaacg gcaccaggct
      361 gatgtctcaa gcgaagaagc ggcggagtaa gaaggatatc agggaatcct tctcgcctct
      421 ctcgcttgtg ccgaccggag atacaactta ggggcaagag atagcaaagt cgaggttcgc
      481 gccgtgagtg cttgacgctg cttgcagccc aaattacaat cctgggaaat tcagcggcat
      541 tgtaaccccg tatctcgcat acaaatcgta gaggaaaaca gtatgagcgc gaaaaatccc
      601 aacccagcct ttggtgccca cctggagcca tttcatggga tcacgacctt catgcgcctg
      661 ccagccagcc gtcagctgga gggcgtggat gtcgccatca caggcatccc cttcgacagt
      721 ggcacaagtt accggagcgg cacccgcttt gggcctcgca aaattcgaga atcttccttg
      781 ctgctgtggg gattcaacac cgtccttggc gtctccccag cggaaacgct caagattgtc
      841 gattacgggg atatcgagat tatcccggtt gagatttcaa aaacaatggg caatatcacc
      901 caggaaatca gccacctgtt gcagaatggc gttacagtga ttgccttggg gggcgaccac
      961 tcggttagcc taccgctact gcgcgcccat gccgcccaat ttggtccgct ggcagtcatc
     1021 cacttcgatt cacaccccga tacctgggat caggaatttc cagggcagcc ttacagccat
     1081 ggcacaccct ttcgccgtgc catcgaggag ggcttgatcg atgtcaatgc ttatttgcag
     1141 attggcatac gcggctcagt cggtcatgcc aatgacctgc cagaggcacg ggaaatgggc
     1201 gcgcgcatct tcaccatcga tcaggtcttc gagctaggta ttccgtccat tattcgtgag
     1261 atgcataaga ctgtagggga tcggaaagtt tatatctccc tggacatcga ctccgtcgat
     1321 ccagcttttg cgccaggtac aggcacgcca gaagtagggg ggttatccag ctatcacatc
     1381 ttgcagctcg tgcgcggcct gcaggggatg aatttcgtgg gattcgacct ggtagaagtg
     1441 agtcccccat atgaccatgg agatatcacc tccatccttg cggcaaacct ggtgttcgaa
     1501 tttttatccc tgctggcgtt acgatcccga aatcgccccg gcgcatgaaa ttccatgagg
     1561 cgcgataatc ctgacctcaa acatgccctg acaggacaat tccgtacccc tgatcactgc
     1621 cccaagcggg cctccatcct cgccaccttt catttttgca aggcgcgcgc aatttctggc
     1681 tgtcaatcag gtttgctgta acactggaac cagcaatgcg tttaaccaat aatagaaaaa
     1741 ttttttggct tcgataaaag caaacctgct gtaaagcagg gacgcaaagc caggggtctc
     1801 gatagagttc gagattgccc ggttgccgaa aagcctcatt gcctgccatc aatgatccag
     1861 gaaggtgagg ctttttgctt tctttatttg cctctcggga agctcgtccc ttcaatggga
     1921 cgacagcctt ccagataggc ttgtagcgag agaaaaggcg tcgaactggt tggcccacca
     1981 gttcaacgcc tctcagacaa agcaccatac ccgatccacg gcaggagcac gtcaggtact
     2041 ggctgccagg gatatttgca gatgatcgtc atctcgacca cagaggcatt gccgtgtctt
     2101 gcgcagattg taaccgaaat aagcaacctt attcaccgtg cagacctaag agatttgtaa
     2161 gcgcgcgccc ggacatagcg acaacaccgg atcccaaccc tgatcatccc gtcactgaaa
     2221 aatggggtat ttaagaaaga cgcagtgtag gagaatccct tggacctaat catcaaggcc
     2281 gctaacgcga cagcgggtaa aatactgatc gtggatgacg atcccgatac ctgccagttg
     2341 ctgcaatttc tctttcagcg gaaaggttac caaaccaagg cggtcaccag tggctggcag
     2401 gccatacagc acgtggagga ccagcccccc gacctggtgg tgctggatgt gctcatgccc
     2461 gagatggacg gctgggagac cttccgccgc gtgcgggagc tatccaattc cctggttctc
     2521 tttctctcgg ctctgggtgg gggagagcac gccgcccagg cgctacaaat gggcgccgac
     2581 gacttcatcc gcaaacccta ctcgaacgta gagctgctgg cgcgcgtcga gatggctctc
     2641 tcccgcgcca ggggcaacac cagccgcgcc tagattgcgc catttccacg aagaaatctc
     2701 agcgcagaat ctccccaaat cctgagaaga caaccagacc aatagcgaca gcgaggccag
     2761 gatgccagtc agccgccagg gcaattgcat atgcactgag aggcatgcgc aaacccgcaa
     2821 ccctagcagc tttcaggtgt tacttttata aagcatctat tgttaagaaa aaggaggaat
     2881 ttatcatgag tttgcgcaat ctattcatcg cttcttcaat cattggcttc attttcggtt
     2941 taggctttta tcttgctcca gcccaaacga tcggcccgta tggtgtagaa ccaccaaccc
     3001 aatcgaccct gtatttcatc cgttttgttg gcgcgtcttt tattggattt agcttgttgg
     3061 catggctggt caaggatgaa ccggcttcgg atacccgcca gaaaatcctt ctgagctact
     3121 ttgtctccta tctcctcata ttgatcgtca tgctgattga gcagttgacg actccggtgg
     3181 gcgcgctggt ttgggtgaat attgttatcc aggcgctgtt taccgctggc tttgggtatt
     3241 accgttttat aaaaacttcg taggctcagc aaccaggtct ggtttttccc acaggaggtg
     3301 cgcgcgcggc gcttccttct gggcgcgcgc ccagccccct ttcttgaccg acacccgtaa
     3361 ttactataca atccataaca gatttgcacc ccatccacac ctcgagctcg acacctcccg
     3421 catagcacgc tatcgccgca aatcgatccg atccgcccga gcgaggcggg cgcccacctc
     3481 caagcttgaa aggtgatgag cagggcactt cctgcgtttt ccaccaggcc atttctcgtt
     3541 agccttcata gtctcaacgc atacaaatta ggagcaaccc tatgtattct ctgaacga
//
LOCUS       DBNGSX010000514        19113 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_190166, whole genome shotgun sequence.
ACCESSION   DBNGSX010000514 DBNGSX010000000
VERSION     DBNGSX010000514.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 19113)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19113)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19113
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_190166"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1524)
                     /gene="yabN"
                     /locus_tag="AC16CB_15205"
     CDS             complement(64..1524)
                     /gene="yabN"
                     /locus_tag="AC16CB_15205"
                     /inference="COORDINATES: protein motif:HMM:NF052436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     methyltransferase/pyrophosphohydrolase YabN"
                     /protein_id="HIR3343869.1"
                     /translation="MDNSSHGGIILLGLGPGDPALLTRQAWELLNNIQVIHLRTSQHP
                     VVAGFPPSLRVLSFDDLYERAENFEDVYTQIIARILELGKSPDGVIYAVPGHPFVAEA
                     TCPEIARRARLDGIPVRVLEGISFLEPCLTALELDPFPHLALIDALELASAHVPSFPP
                     SSPAIVAQIHSRAVASDVKLTLMSLYPDEHPVKLVHAAGTPDELVEAIPLYEIDRSQH
                     IGLLTALYVPPLGAATSFEAFQEVIAHLRAPDGCPWDKEQTHQTLRSHLIEESYELLA
                     ALDAGDPQAMREEFGDLLLQIVLHTQIASEFGEFTMSDVLESIHTKIVRRHPHVFGNL
                     ALKDVQGVLENWERLKEAERNANGKAEAGLLDGVAQALPALVQAQEYQKRAGRVGFDW
                     PNIQGVIDKVHEELREVNAARTANDRAAEIGDLLFAVVNLARWVDVDAESALREANAR
                     FRSRFAQIEAAARAQNRSITDLSLDEMEALWQAAKK"
     gene            complement(1615..2010)
                     /gene="rpsI"
                     /locus_tag="AC16CB_15210"
     CDS             complement(1615..2010)
                     /gene="rpsI"
                     /locus_tag="AC16CB_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR3343870.1"
                     /translation="MSTQFYEGIGRRKQATARVRIMSGSGNFTVNEKPAEAYFTRLGD
                     LEQIVAPLKAAGENPNQLDISVVVDGGGVTGQTDAVQLGVARAILLMNPDMRSTLRRG
                     GYLTRDARVKERKKPGLKRARKAPTYTKR"
     gene            complement(2023..2451)
                     /gene="rplM"
                     /locus_tag="AC16CB_15215"
     CDS             complement(2023..2451)
                     /gene="rplM"
                     /locus_tag="AC16CB_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR3343871.1"
                     /translation="MEKTYYPKAGEISQDWILVDANDQNLGRLATHIATLLLGKHKPN
                     FTPGVEMGDYVVVINAERIRVTGKKLVEKKYYRHSNYPGGLKTISLQDQLAKHPDRVI
                     KSAVWGMLPHNKVGRRLLKNLKIYAGPEHPHAAQTPKSLS"
     gene            complement(2557..3318)
                     /gene="truA"
                     /locus_tag="AC16CB_15220"
     CDS             complement(2557..3318)
                     /gene="truA"
                     /locus_tag="AC16CB_15220"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR3343872.1"
                     /translation="MARYQVILAYDGTQYHGFQRQAKAHTVQGVIEATLRELGWSGRA
                     ILAAGRTDTGVHASGQVIAFDLDWKHSPDELGAALNAQLPDDVAARSVMQVEVDFHPR
                     YDALARTYRYRIYCWENRDPLLDRYAWRVWPALDLDLLQQAASLLVGRQDFAAFGSPP
                     RPSGSTQRTVFQAQWTRYDHELIFLITADAFLYRMVRRLVSFQVEIGQGRRELEALRQ
                     VLELPPDSAVQGLAPAHGLTLLKVVYPSDEARILI"
     gene            complement(3468..3818)
                     /gene="rplQ"
                     /locus_tag="AC16CB_15225"
     CDS             complement(3468..3818)
                     /gene="rplQ"
                     /locus_tag="AC16CB_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011369741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIR3343873.1"
                     /translation="MSRGKDERTALRRILIKQLFEHERIRTTRAKARAVRGQAERLIT
                     LAKRGNQAGEEKAVHARRLAAARLGDPVAVKKLFDEIAPRFESRPGGYTRILSLGPRL
                     GDAADMVILELIED"
     gene            complement(3850..4803)
                     /locus_tag="AC16CB_15230"
     CDS             complement(3850..4803)
                     /locus_tag="AC16CB_15230"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR3343874.1"
                     /translation="MVTPKIERKAEARNYGKFIISPLERGYGVTLGNALRRVLLSSLD
                     GAAVTSIRFADVQHEFSDVPGVREDVIRIMLQIKQLRMKLSDVESARMHLEVRGAGVV
                     TAADIITPPEVEIINPDLYLFTVDDNNANLEIEMTVERGRGYSPADDRTGRLPIGELP
                     VDAIYSPVRRVNWDVGLARVGQSTNYDSLVMEIWTDGTIGPEESLSTGAKTLIDHLRH
                     LAGVSEESLASLAEAQEEAGRLTSEIIETPIENLDLSVRVFNSLKRTGITTIGEVIEL
                     LEKGDEAVMSIRNFGEKSLDELRQRMQEKGYLKDDEESEAE"
     gene            complement(4927..5568)
                     /gene="rpsD"
                     /locus_tag="AC16CB_15235"
     CDS             complement(4927..5568)
                     /gene="rpsD"
                     /locus_tag="AC16CB_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR3343875.1"
                     /translation="MAKYRGPVCKLCRREGEKLFLKGERCFTPKCAFERRGYPPGQHG
                     KGAQFRRRRESDFNRQLRAKQKARRVYGVLERQFRRYYETSLSRRGLTGLNLLQILES
                     RFDNVVFRLGFADSRAQARLLVTHGHFAVNGRRTDIPSMLLSPGDEISVREGNSRTRP
                     YFKDLAAVAETKNIPAWLERDLKALSGKVLRLPERAEIDGNLHEQLIVEYYSR"
     gene            complement(5600..6001)
                     /gene="rpsK"
                     /locus_tag="AC16CB_15240"
     CDS             complement(5600..6001)
                     /gene="rpsK"
                     /locus_tag="AC16CB_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014015761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIR3343876.1"
                     /translation="MAQSVRTARRKGTKKIKRTLSSGQIHILATFNNTIITVTDPSGN
                     TVAWGSAGSAGFKGSRKSTPFAARLAAEQALKAAMALGLQEVQVFVKGPGPGRESAVR
                     AIQTLGLKVTSISDVTPVPHNGCRPPKKRRV"
     gene            complement(6025..6414)
                     /gene="rpsM"
                     /locus_tag="AC16CB_15245"
     CDS             complement(6025..6414)
                     /gene="rpsM"
                     /locus_tag="AC16CB_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIR3343877.1"
                     /translation="MARIEGVDLPRNKRVEVGLTYIYGIGLPRAQQILKATGVNPDIR
                     IKDLTDADVTLLREFISQNYKLEGDLRREVQLNIKRLIEIGSYRGLRHRRNLPVRGQR
                     TRTNARTRKGPKKTVAGRGRRRGAKKK"
     gene            complement(6421..6534)
                     /gene="rpmJ"
                     /locus_tag="AC16CB_15250"
     CDS             complement(6421..6534)
                     /gene="rpmJ"
                     /locus_tag="AC16CB_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIR3343878.1"
                     /translation="MKVSASIKKRCSKCKIVKRKGVLYVICENSKHKQRQG"
     gene            complement(6672..7442)
                     /gene="map"
                     /locus_tag="AC16CB_15255"
     CDS             complement(6672..7442)
                     /gene="map"
                     /locus_tag="AC16CB_15255"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR3343879.1"
                     /translation="MISQRGIFIKSQQEITIMREAGRINALALDAVRQAIRPGVTTAE
                     LDQIAADVIRKHGAKPAFLGVPGAYPYPATTTISVNEEMVHGIPGKRKLQEGDIISVD
                     CGTIFEGYVGDSAFTMGVGEVSQVAQQLMEVTEKSLHIGIDQLRPGKRVGDVGAAIQE
                     YVEGQGFHLTREYTGHGVGRAMWEGPQVPNYGVSGRGTVFRPGMVIALEPMVLVGTTH
                     TRVLPDQWTVASADGSLTAHFEHSVAVTQNGPLVLTIP"
     gene            complement(7445..8101)
                     /locus_tag="AC16CB_15260"
     CDS             complement(7445..8101)
                     /locus_tag="AC16CB_15260"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIR3343880.1"
                     /translation="MPKYIVLLGPPGAGKGTQAQIISNELGLPHISSGDLFRENLKGQ
                     TELGKLAKGYIDRGELVPDDVTISMVRERLSRPDCSSGALLDGFPRTPAQAEALARML
                     DDFQSQVDVVPYISVPEPVLIERLTGRWTCRANGHVFHEKFNPPREAGRCDFDGSQLY
                     QREDDKEETVINRIHVYLKQTMPLIEYYRQQGLLLEVDGSQPIEKVSMYLLAALPAGK
                     "
     gene            complement(8114..9469)
                     /gene="secY"
                     /locus_tag="AC16CB_15265"
     CDS             complement(8114..9469)
                     /gene="secY"
                     /locus_tag="AC16CB_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR3343881.1"
                     /translation="MKRSAWRFLWYAKDIRRKLLVTVGLLAIFRLAAHVPVPGANREA
                     IASIIAGGGAGGTLFSLLNMLSGGTVTNFSVLAMGVYPYITAQIILQLLVPIIPALQR
                     RMEEDPREGQKWMEKWTYMLAVPMAALQAIGQINLFNAFTGGVPIISDFGFSGANLLP
                     SITIILSMTAGTMFAIWLGELISEYGIRNQGLSLIIFAGIVSDVPTNLLRLLQDERTR
                     VFVPIFIAIMMALIIFAIVYVQQGRRNVPVMYPGRRVGNRMSMPVKGTLPLMVNMAGM
                     IPLIFAQSLLTFPAIIASFFVSSQIAWVSNTAATVQNLFGGNSNAYWIFYFLMVVGFT
                     FFYTDVLFAQQNYGENLKRVGAQIPGVSRGAPTQKYLTRVLRRITLPGALFLGLVAVL
                     PVLVTLILSGFGVSLGGQSGILLVSSAGLLIVVGVVRDTFFNIEAELKLHGYDDTQLI
                     R"
     gene            complement(9515..9970)
                     /gene="rplO"
                     /locus_tag="AC16CB_15270"
     CDS             complement(9515..9970)
                     /gene="rplO"
                     /locus_tag="AC16CB_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIR3343882.1"
                     /translation="MKLHDLQPNPGSKKSRKRVGRGTAAGQGKNAGRGVKGQGARAGG
                     GVRLYHQGGNLPFYRRLPFVRGQGFTPLFRQEYNEVNLDQLADFPADSEVTPETLETA
                     RLLHEPENPVVILGRGEVSVALKVSAHRVSKGAQAKIEAAGGSVEILEL"
     gene            complement(9975..10181)
                     /gene="rpmD"
                     /locus_tag="AC16CB_15275"
     CDS             complement(9975..10181)
                     /gene="rpmD"
                     /locus_tag="AC16CB_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIR3343883.1"
                     /translation="MARKAKVVEKRLRVTLVKSPIGYTQRQKDTVRALGLHRMHATVE
                     HIDSASVRGMIAKVSHLVDVEEVE"
     gene            complement(10174..10701)
                     /gene="rpsE"
                     /locus_tag="AC16CB_15280"
     CDS             complement(10174..10701)
                     /gene="rpsE"
                     /locus_tag="AC16CB_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIR3343884.1"
                     /translation="MTDYNEFDQLELDERVVEIARVAKVVKGGRRFSFRVTVVVGDNR
                     GSIGVGIGKANAVPDAMRKATDKARRNMQKVNMFGTTIPHEVMGIMAGSKVLLKPASP
                     GTGVIAGGGVRAVLEAVGVSDILTKSLGSSNVLNVVYATINALEQLKSPQEVAAFRGK
                     AVKDVTPFWERRKNG"
     gene            complement(10735..11103)
                     /gene="rplR"
                     /locus_tag="AC16CB_15285"
     CDS             complement(10735..11103)
                     /gene="rplR"
                     /locus_tag="AC16CB_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013429978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIR3343885.1"
                     /translation="MGTIKSRSVARLRRHRRVRKNVSGTPEKPRLNVFRSLAEIYAQV
                     IDDEAGHTLVSASSVDRHLRDTLKGKKKSEQAHLVGKALAERAKEKGISIVVFDRGGY
                     RYIGRVKALADGAREGGMEF"
     gene            complement(11107..11649)
                     /gene="rplF"
                     /locus_tag="AC16CB_15290"
     CDS             complement(11107..11649)
                     /gene="rplF"
                     /locus_tag="AC16CB_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010896332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIR3343886.1"
                     /translation="MSRIGRAPVDVPNGVDVKIKGSNVRVKGPKGELARTFPADIIIS
                     VEDGKIVVKRPSDEPRHRALHGMTRALINNMVVGVSDGFEKILEVNGVGYRAELDGEN
                     LVLYVGYSHSVKMEPPGGISFEVDARTRQIKVMGVDKEMVGQVAANIRMVRPPEPYKG
                     KGIKYLDERIRRKAGKAGKV"
     gene            complement(11661..12074)
                     /gene="rpsH"
                     /locus_tag="AC16CB_15295"
     CDS             complement(11661..12074)
                     /gene="rpsH"
                     /locus_tag="AC16CB_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIR3343887.1"
                     /translation="MSVNDPIADMLTRIRNAVMVGHQMVALPSSKLKVAIAKILKDEG
                     FITSFEVVDGKTVGQKVLRIRLKYIGERRERKPVITGLERISRPGRRVYTNKREIPWV
                     LSGMGVAIISTPKGVMTGQRARQLGVGGEILCKVW"
     gene            complement(12094..12276)
                     /locus_tag="AC16CB_15300"
     CDS             complement(12094..12276)
                     /locus_tag="AC16CB_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002557082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HIR3343888.1"
                     /translation="MAKKCMMIRETRRKYPTRVRNRCKQCGRPRGYLRRFGLCRICFR
                     ELALEGKIPGVVKSSW"
     gene            complement(12280..12831)
                     /gene="rplE"
                     /locus_tag="AC16CB_15305"
     CDS             complement(12280..12831)
                     /gene="rplE"
                     /locus_tag="AC16CB_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR3343889.1"
                     /translation="MMGHILKERYQEEIVPALMKSLHLENTMQAPRIQKIVVNVGVGE
                     ALDNAKALDATVADVTQVTGQKPIVTKARKSIANFKLREGRQIGVKVTLRGDRMWAFL
                     DRLMNVALPRVRDFRGISPNAFDGRGNYTLGLREQLVFPEIDYDKIDKLRGMEISIVT
                     TGRNDEEGRQLLQLLGMPFKKEG"
     gene            complement(12853..13188)
                     /gene="rplX"
                     /locus_tag="AC16CB_15310"
     CDS             complement(12853..13188)
                     /gene="rplX"
                     /locus_tag="AC16CB_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIR3343890.1"
                     /translation="MRKKIRKGDMVEVINGRFEDKGKRGEVIRVNPDDGRVVVQGINI
                     RKKHQRQVQTQGRRNLSPGIIEFEGPIHISNVMLVCPKCSEPTRVGLQREGDESLRVC
                     KHCHGLIDV"
     gene            complement(13215..13586)
                     /gene="rplN"
                     /locus_tag="AC16CB_15315"
     CDS             complement(13215..13586)
                     /gene="rplN"
                     /locus_tag="AC16CB_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIR3343891.1"
                     /translation="MIQQESRLKVADNTGARELLVIHVMGGSTRRYARVGDVVVATVK
                     AATPQGSVKKSDIVRAVVVRCAKEWRREDGSYIRFDDNAAVILDQDGRNPKGTRIFGP
                     VARELREKGFMKIVSLAPEVL"
     gene            complement(13583..13870)
                     /gene="rpsQ"
                     /locus_tag="AC16CB_15320"
     CDS             complement(13583..13870)
                     /gene="rpsQ"
                     /locus_tag="AC16CB_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIR3343892.1"
                     /translation="MNTRRRLTGVVTSDKMDKTVVVEISRTYRHRLYKKVVDSSKRIM
                     AHDELGCQVGDHVRIVESRPISRHKHWVVEEILRRAIGADEPEVGLLENVP"
     gene            complement(13867..14079)
                     /gene="rpmC"
                     /locus_tag="AC16CB_15325"
     CDS             complement(13867..14079)
                     /gene="rpmC"
                     /locus_tag="AC16CB_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIR3343893.1"
                     /translation="MKPEEIRALSTEDIQARLDDAKEELMKLRFQLAMGGLNDYTRLR
                     YTRKSVARLMTILNERQFTAQEEGEA"
     gene            complement(14076..14510)
                     /gene="rplP"
                     /locus_tag="AC16CB_15330"
     CDS             complement(14076..14510)
                     /gene="rplP"
                     /locus_tag="AC16CB_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIR3343894.1"
                     /translation="MLMPKRVKWRKQMRGRMRGKAQRGAQISFGEFGLQAMEPGWVSA
                     RQIEAARRAIVRALRRRGKVWIRIFPDKPVTRKAAETRMGKGKGNVEYWVAVVKPGRV
                     MFEVGGGLTPDVAQEALRLAQYKLAIKTKIIGRYEETGGGEA"
     gene            complement(14520..15197)
                     /gene="rpsC"
                     /locus_tag="AC16CB_15335"
     CDS             complement(14520..15197)
                     /gene="rpsC"
                     /locus_tag="AC16CB_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIR3343895.1"
                     /translation="MGRKVHPTGFRLKINKTWEGRWYAEGEDYVNQLHQDLSLRELVR
                     QEADRAGVSRIEVERFPGKVKVLVHTAKPGILIGRKGENVKKIRSNLEVLVGKKIDLE
                     IKEIKSPDLDAYLVARNIADQLERRISYQRAMKRAIQQAIRQGAEGIRLEVSGRLSGA
                     EMARAVNMREGQVPRQTLRADIDFSKAEANTTYGRIGVKVWIYKGEVLPEMEDKLEST
                     EGVYVSE"
     gene            complement(15228..15569)
                     /gene="rplV"
                     /locus_tag="AC16CB_15340"
     CDS             complement(15228..15569)
                     /gene="rplV"
                     /locus_tag="AC16CB_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIR3343896.1"
                     /translation="METDISAHARFIPHSAQKVRLVIDLVRGKNVSEALSTLKFITNA
                     AAVPVSKALASAVANAEENFGVSRDDLFVYSIYANEAPTRKWRRFGARGRFKPWLRRS
                     SHITVILRERE"
     gene            complement(15573..15854)
                     /gene="rpsS"
                     /locus_tag="AC16CB_15345"
     CDS             complement(15573..15854)
                     /gene="rpsS"
                     /locus_tag="AC16CB_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIR3343897.1"
                     /translation="MGRSLKKGPFIDPKLLKKVEVMNERGDKKVIRTWSRASTIFPQM
                     VGHTVAVHDGRRHVPIYITENMVGHRLGEFAPTRTFRGHLAEKKIKKGS"
     gene            complement(15872..16708)
                     /gene="rplB"
                     /locus_tag="AC16CB_15350"
     CDS             complement(15872..16708)
                     /gene="rplB"
                     /locus_tag="AC16CB_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIR3343898.1"
                     /translation="MAVKKYKPTTPGQRGMTGSTFEEITKKSPERSLLKPLRKSGGRN
                     LYGRITVRHRGGGHKRHIRIVDFKRDKRGVPAKVAAIEYDPNRTARLALLNYVDGEKR
                     YIIAPVDLKVGDTVLAGPGAEIRPGNSLPIANIPVGTMVHNIELKVGRGGQLVRAAGG
                     AAQLLAKEGDYAQVRMPSGEVRLVRQECYASIGQVGNLDHGNIKLGKAGRKRHKGIRP
                     AVRGSAMSPRDHPHGGGEGRQPIGMPGPKSPWGKPTLGYKTRRNKKTDQYIVRRRGKK
                     RR"
     gene            complement(16708..17010)
                     /gene="rplW"
                     /locus_tag="AC16CB_15355"
     CDS             complement(16708..17010)
                     /gene="rplW"
                     /locus_tag="AC16CB_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIR3343899.1"
                     /translation="MTSIYDVLRRPIVTEKSNYLNSDLHQYVFEVSSDATKHMVKDAV
                     ETLFDVDVVRVNIVNVPARRTRRIRSRRVLVRRGAYKKAIVTLAPEDSIDIFEGVK"
     gene            complement(17024..17656)
                     /gene="rplD"
                     /locus_tag="AC16CB_15360"
     CDS             complement(17024..17656)
                     /gene="rplD"
                     /locus_tag="AC16CB_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIR3343900.1"
                     /translation="MMEVDVLNMEGQKVSTVELPSLIFEAPVNVDLMHQAYVRQMANA
                     RLGTHKTKGRSEVSGGGRKPWRQKGTGRARQGSTRAAQWVGGGKIHTPKPRDYSMQMP
                     RKMRRAALRSALSVKAAESSIVVLDELSVSEPKTRLMAQALDQLVGDASVLILIPEST
                     TGYDMVIRSTNNLPDTKMLQANYLNIRDLLGYDKVLMPLKVLDVLVSFLG"
     gene            complement(17706..18338)
                     /gene="rplC"
                     /locus_tag="AC16CB_15365"
     CDS             complement(17706..18338)
                     /gene="rplC"
                     /locus_tag="AC16CB_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIR3343901.1"
                     /translation="MLKGLIGKKIGMTQIFDDAGVAIPVTLIEAGPCYVTQVRLPEVD
                     GYSAVQLGFVEVKQKRLTSGQLGHLKRNNTPPLRFLREFRHKGPEVKEGDTLTVDAVF
                     NVGENVDVIGTSKGKGFAGSVKRHHFRGGPKTHGQSDRHRAPGSRGSGTTPGRVYKGS
                     RGAGHMGNERVTVQNLKVVLIDSERNLMGVRGAIPGPKGGIVMIKEARKR"
     gene            complement(18544..18852)
                     /gene="rpsJ"
                     /locus_tag="AC16CB_15370"
     CDS             complement(18544..18852)
                     /gene="rpsJ"
                     /locus_tag="AC16CB_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIR3343902.1"
                     /translation="MAKQKIRIRLKAYDHRVLDQSAKRIVETAERTGARVVGPVPLPT
                     RIERFTVRRSTFIDKDSHEHFEVRTHNRLIDVLDPDSKTIDMLMRLNLPAGVDIEIKI
                     "
     gene            complement(18863..>19113)
                     /locus_tag="AC16CB_15375"
     CDS             complement(18863..>19113)
                     /locus_tag="AC16CB_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu C-terminal domain-related protein"
                     /protein_id="HIR3343903.1"
                     /translation="RKEEGGRHKAFFTGYRPQFYIRTMDVTGNITLPEGVEMVMPGDN
                     VNLEVELIVPVALEQGSKFAIREGGLTVGAGVITKILD"
BASE COUNT         4279 a         5609 c         4620 g         4605 t
ORIGIN      
        1 tttttggtca agcccccaga ggggtcactc cgctattcct gcctctggtg agcaggtaag
       61 atttcatttt ttcgcggcct gccacagggc ttccatctcg tccaggctca ggtcagtgat
      121 cgagcggttt tgggctcgtg ccgcggcctc aatttgagca aaacgactgc ggaaacgcgc
      181 gtttgcctcg cgcaaggcgc tctcagcatc tacatcaacc cagcgtgcca ggttgaccac
      241 cgcaaagagc aggtcgccaa tctcagccgc ccggtcatta gccgtacggg cagcattgac
      301 ttccctgagc tcctcgtgca ctttgtcaat caccccctgg atatttggcc aatcgaaacc
      361 aacccgaccg gctcgcttct ggtattcctg tgcctgcacc agcgcgggaa gcgcctgtgc
      421 aacgccatcc agcaagccag cctcagcttt gccattggca ttgcgctcag cttcctttag
      481 gcgctcccag ttctctaaca ctccctgtac atctttcaaa gccaggttgc caaagacatg
      541 cgggtggcgg cgcacgatct ttgtgtgaat actttctagc acgtccgaca tggtgaactc
      601 gccgaattcg ctggcaattt gggtatgcag cacaatctgc agcagcaggt cgccgaactc
      661 ctcgcgcata gcctgtggat cgcctgcatc cagcgcagcc agtaactcgt aactttcctc
      721 gatcaggtga gagcggagcg tttggtgggt ctgctccttg tcccaagggc agccatctgg
      781 cgcgcgcagg tgggcgatca cctcctgaaa agcctcaaag gaggtcgcgg cacctaatgg
      841 gggaacatac aaggcggtga gcagcccaat gtgctggcta cggtcaattt cataaagcgg
      901 gattgcttcc accaattcat cgggcgtgcc tgccgcatgc accagcttga ccggatgctc
      961 gtcagggtac aggctcatca aagtgagctt gacgtccgaa gcaaccgccc gcgagtgtat
     1021 ttgggcgacg attgccgggg agcttggcgg gaaggatgga acatgggcag atgccagctc
     1081 cagggcgtca atgagagcca ggtggggaaa ggggtctaat tcaagagccg tcaggcatgg
     1141 ctccagaaag ctaatcccct ccaatacgcg caccgggatg ccatccaagc gagcgcggcg
     1201 ggcaatctcc ggacaggtgg cttcggccac gaaaggatgc cccgggacgg catatatcac
     1261 accatctggc gacttcccca gctctaagat gcgagcaatg atctgcgtgt aaacatcttc
     1321 gaaattttct gcgcgctcat acagatcatc gaatgatagt accctcaggc tgggagggaa
     1381 acctgccact acgggatgct ggctggtgcg caggtgaatt acctggatgt tattaagaag
     1441 ctcccatgcc tgccgggtga gcaaggccgg atcgcctggg ccaagcccaa gaagaataat
     1501 tccaccatgt gatgagttgt ccatagccac tattttaaaa gacttttggg gtaatgggca
     1561 gatctcgcct gcgcattacc ccaatatcat tcttaagccg tgaaattgta cgaattaccg
     1621 cttggtgtag gtgggagcct tgcgagcccg cttcagaccg ggcttcttgc gctccttgac
     1681 acgcgcgtca cgggtcaggt atccgcctcg acggagcgtc gagcgcatat cagggttcat
     1741 caagagaatg gcgcgcgcca cgcccaattg gacagcgtct gtttgccctg tgacaccccc
     1801 gccatcaacc acaacggata tatccagctg gttagggttt tctccagccg cttttagggg
     1861 agcgacaatt tgttccaaat cacccaagcg ggtgaagtaa gcctcggctg gcttctcatt
     1921 gaccgtgaaa tttcccgatc ctgacatgat gcgcacgcgc gcggtagctt gcttgcggcg
     1981 accaatgcct tcataaaatt gcgtcgacat gcttcttgct ccttaagaca atgacttggg
     2041 tgtctgggca gcatgtggat gttcgggccc agcataaatt ttcaagtttt tcaacaaccg
     2101 cctgccgact ttattgtgcg gcagcatacc ccatacagcg cttttgatca ctcgatccgg
     2161 atgcttagcg agctgatcct gcaggctgat ggtcttcagc ccaccggggt aattggaatg
     2221 gcgataatat tttttctcaa ccaacttctt tccggttacg cggatgcgct ccgcattaat
     2281 aacgacgacg taatctccca tctccacgcc cggagtgaag ttcggcttat gcttacccag
     2341 caaaagagta gcaatatgtg tcgccagacg tcccaggttt tgatcatttg cgtcaaccag
     2401 gatccaatct tggctgattt caccagcttt tggataataa gttttttcca cgttcaaatt
     2461 ctccgctatc ttccagtcat ttagcctgac aatttctgat ctatttcatc aatgaccgcg
     2521 tgatgcagtt agattgataa cttaactgca tatttcctaa atgagtatcc tcgcttcatc
     2581 ggatgggtag accaccttta gcagggttag cccatgggct ggcgccaagc cttgaaccgc
     2641 tgagtctgga ggtaactcca gcacttgcct taacgcttca agctcacgcc ttccctgccc
     2701 gatctccacc tggaagctga ccaatctccg caccatccga taaagaaatg catcagcagt
     2761 gatcaaaaaa atcagctcgt ggtcataccg cgtccattgt gcctgaaaga ccgtccgctg
     2821 tgtgcttcct gaagggcgag gcggggaacc aaatgcggca aaatcctgcc ttccgaccaa
     2881 gagactcgca gcctgttgga gcaagtcgag atccagggca ggccacaccc gccaggcgta
     2941 tcgatccagc aacgggtcac gattttccca gcaatagatc cggtagcgat aagtacgggc
     3001 aagcgcatcg tagcgaggat ggaaatccac ctcaacttgc attacagacc gagcagccac
     3061 atcgtctggc agttgcgcat tcagtgcagc acccaactcg tcaggggaat gcttccaatc
     3121 cagatcaaaa gcaatcacct gcccggaggc atgcacacca gtgtctgtgc gcccagctgc
     3181 aagaatggcc cgaccactcc agcctaattc ccgcaatgtc gcctcaatca caccttgcac
     3241 agtatgtgcc ttggcttggc gttgaaagcc atggtactgg gtgccatcat aggcgaggat
     3301 aacttggtaa cgtgccattt tcagttgtcg gtgatcaaat gttgcttagt cgaggacgat
     3361 aaatacggtg tagcaatcat cgatctctga caactggcga ttgactagaa ctcatctaaa
     3421 catttctggg ttggggtaga gacacccctc gcttttttag ataaattcta atcttcgata
     3481 agctccaaaa tcaccatatc agccgcgtcg cctaaacgcg ggcctaggct cagaatgcgc
     3541 gtatagccac ctgggcggga ttcaaaacgc ggggcaattt catcgaacag cttcttgacc
     3601 gccacgggat cgcccaagcg ggcagccgcc aagcggcggg cgtgcactgc tttctcctca
     3661 ccagcctggt tgccgcgctt agcaagcgta atcaatcgct ctgcctggcc tcgcaccgcg
     3721 cgagccttcg cgcgggttgt gcgaatgcgc tcgtgctcaa ataattgctt gattaaaatt
     3781 cgccgcagag cggtgcgctc atcctttccg cggctcaatc tatatcctac gattttgtgt
     3841 cgcataccgt cactccgctt cgctctcttc gtcgtctttg aggtagccct tctcctgcat
     3901 cctctggcgt aactcgtcca gacttttctc gccgaaatta cggatggaca tcaccgcctc
     3961 atcacccttt tcaagcagct cgatcacctc accgatggtg gtgatgcccg tgcgcttgag
     4021 tgagttgaaa acccgcaccg acaggtccag gttttcgatg ggcgtctcga tgatctcgct
     4081 ggtcagcctg cctgcctctt cttgggcttc tgcgagcgag gcaagcgatt cttcgctgac
     4141 acccgccaga tggcgaagat gatcaatgag cgttttggcg ccggtgctga gtgattcctc
     4201 tggtccgatg gtcccatccg tccagatctc catcaccagg ctgtcatagt ttgtgctctg
     4261 acccacgcgc gccaagccaa catcccagtt gacccgacgc acagggctat agatggcatc
     4321 tacgggtagt tcgccaatcg gcaagcgccc agtacgatca tctgcgggtg agtaacccct
     4381 tccccgttca acagtcatct caatttctaa attggcgttg ttatcatcca ccgtgaagag
     4441 gtacagatca ggattaatga tctccacttc aggcggcgtg atgatatcag ccgcggtgac
     4501 cactcctgca cctcgtacct ctaggtgcat gcgagcggat tccacgtcac tcaatttcat
     4561 gcgcaattgc ttgatctgca acattatccg gatcacatct tcacggacac caggtacatc
     4621 gctaaattcg tgttgcacat ccgcaaagcg aattgaggtg accgccgcgc catcgagcga
     4681 ggagagcaga acccggcgga gggcgttgcc taacgtgaca ccatagccgc gctccagcgg
     4741 gctgataatg aacttgccgt agttgcgcgc ctccgctttc cgctcaattt tcggcgttac
     4801 catattactt aaacctatca cggttcacct cgaaattgat tgaagacagg caggttacaa
     4861 gctaaagata taccaggaca agctatggcg taatcaataa ctttcaacct tccagcttgc
     4921 aatccattat ctagaatagt actcaacgat gagttgctcg tgcaaattcc catcgatctc
     4981 agcccgctcc ggcaggcgca ataccttgcc cgatagagcc ttaaggtctc tttccagcca
     5041 ggcggggata tttttcgtct cggccacggc tgctaagtcc ttgaagtatg gtcgggtgcg
     5101 agagttgcct tcacgcacag agatctcgtc gccgggagag agcaacatcg aagggatgtc
     5161 tgtgcgccgt ccattgacgg cgaagtgccc gtgtgtcacc aagaggcgcg cctgagcacg
     5221 gctatcggcg aagcccagac ggaataccac attgtcgaag cgtgactcga ggatctgcaa
     5281 caagtttaga cctgtcaacc cacgacgact tagagaggtc tcatagtaac gacggaactg
     5341 tcgctccaac acgccataca cgcggcgagc tttctgcttg gcgcgcaact ggcgattaaa
     5401 gtctgactcg cgcctacggc ggaactgagc gcctttgcca tgctgaccgg gggggtatcc
     5461 ccgtcgctca aaagcgcatt tcggtgtgaa acagcgctcc cctttcagga acagcttttc
     5521 accttctcgc cggcacaact tacaaactgg cccacgatac tttgccatac gtttcttacc
     5581 ttatccttcc tgaataatat taaacacgcc gcttctttgg tggtcggcag ccattatgag
     5641 gtactggcgt gacgtcagaa atcgatgtca ctttcagacc cagcgtctgg atggcgcgca
     5701 ccgccgattc gcgacctggc ccaggacctt tgacaaagac ctgtacctct tgcaaaccca
     5761 atgccatggc tgccttaaga gcttgttcag ccgccaggcg ggctgcgaag ggtgtgcttt
     5821 ttcgtgaacc tttaaaacct gcagatcctg cgctacccca agccactgta ttaccggacg
     5881 ggtcggttac cgttatgatc gtattattga aggtagccag aatatgaatc tgccccgatg
     5941 atagggttcg tttgattttc tttgtgcctt tgcggcgagc tgtacgaaca ctttgagcca
     6001 tagaacctca caatttccag atgattattt cttctttgct ccacgtcgcc gtccgcgccc
     6061 ggcaactgtt ttctttggac ctttgcgggt gcgggcgttg gtgcgcgtcc tttgcccacg
     6121 gaccggcaaa ttgcgacgat ggcgaagccc gcggtagctg ccaatctcaa tcaagcgctt
     6181 gatattgagc tggacttcgc gtcgcaggtc accctctagc ttatagttct gagagatgaa
     6241 ttcgcgcaac agggtgacat cagcatcggt gagatcttta atgcgaatat cagggtttac
     6301 accggttgcc ttcaagatct gctgcgcgcg gggcaggcca ataccataaa tataggtcag
     6361 acccacttca acccgcttgt tacgaggtaa gtcaacgcct tctatacgag ccatatctga
     6421 ttatccctgt cgctgtttat gtttcgaatt ctcacaaatg acgtataaaa cgcctttgcg
     6481 tttgacaatt ttgcacttcg aacagcgctt tttaatagat gcggatactt tcatttcatt
     6541 tctccttctt attaaaactc agaaagagtt ccattctcct gatagccgaa gttttcatta
     6601 taccgtaatc actttctctc gtccagtttt ctcccaagaa tgcgctatta ttcacagcgg
     6661 gtttgctcaa ctcaagggat tgtcaacaca agcggtccat tctgggtaac cgccacgctg
     6721 tgctcgaaat gagcagtcag cgaaccatcg gcagacgcca ctgtccattg gtcgggtaag
     6781 acacgcgtgt gggtcgtacc caccaatacc attggctcca aggcaatgac catacccggt
     6841 cggaagaccg tgcccctgcc ggaaacaccg taattgggca cctgcggacc ttcccacatc
     6901 gcccgtccta caccatgacc ggtatattcc cgggttaagt gaaatccctg gccctctaca
     6961 tactcctgta tggcagctcc tacatcacca acgcgcttac ccgggcgcaa ctggtcaata
     7021 ccaatgtgca gagatttctc ggtcacctcc atcagctgct gggcaacctg agagacttcg
     7081 cccacaccca tggtaaaagc cgagtcacct acgtatccct cgaagatcgt gccgcaatcc
     7141 actgagatga tatcgccttc ttgcagcttt cgcttccccg gtatgccgtg aaccatctct
     7201 tcgttaacgc taatggtcgt cgtagccggg tatggatacg ctccaggaac tcccaggaaa
     7261 gctggtttgg cgccatgctt tcgaatgacg tccgcagcga tctgatccaa ctcggcagtc
     7321 gtcaccccgg gtcgtatcgc ctggcgcaca gcatccaaag ccaaggcgtt aatccgtcca
     7381 gcctcgcgca tgatggtgat ttcctgttgg ctcttgatga agatgccgcg ttgactgatc
     7441 atgtctattt gcctgcaggg agagcagcca ataaatacat cgataccttc tcgataggtt
     7501 ggcttccgtc cacttccaat agtaagccct gttgacgata gtattcgatc aggggcatcg
     7561 tttgtttaag gtacacatgg atacggttga tcaccgtttc ctctttatca tcctctcgct
     7621 ggtagagctg cgaaccgtcg aaatcacaac gacctgcttc ccgcgggggg ttgaacttct
     7681 cgtgaaagac gtgcccattt gcccggcaag tccagcgtcc ggtcaggcgc tcgatcagca
     7741 cgggctccgg aacactgata taaggcacca catctacctg actttgaaaa tcgtccagca
     7801 ttctcgccag ggcttcggct tgcgccggcg tgcgtggaaa tccatccagc agcgcgcccg
     7861 atgaacaatc tggacgcgag agtcgctcgc gcaccatgga gatggtcaca tcatctggca
     7921 ccagctcacc ccggtcgata tagcccttgg ctagcttgcc taactcggtc tgccccttta
     7981 ggttctcgcg gaacagatct ccagacgaga tatgcggcaa ccccagttca ttggagatga
     8041 tttgcgcctg ggttccctta cctgcacccg gagggccgag taaaacaatg tactttggca
     8101 tgcgtcactc cgcttaacgg atcagctgcg tatcatcgta accgtgaagc ttgagctctg
     8161 cctcaatatt aaagaacgta tcgcgcacga cacccaccac aatcagcaat cctgctgatg
     8221 agaccaatag gatgcccgat tgaccaccca gactgacccc gaaaccagac agaattaagg
     8281 tgaccagcac cggcaaaacc gccaccagac ccaggaataa agccccaggt agggtgatgc
     8341 gccgcaagac acgcgtcaga tatttttggg taggcgcacc ccggctgaca cccgggattt
     8401 gtgcaccgac tcgctttaaa ttctcgccgt aattttgttg ggcgaaaagc acatcagtgt
     8461 aaaagaaagt gaaacctaca accatcaaga agtaaaagat ccagtaggca ttgctattgc
     8521 cgccaaacag attttggacc gtggccgcag tatttgaaac ccacgcaatt tgtgaactga
     8581 caaaaaagct ggcgataatg gctgggaagg tgagcaacga ctgggcgaag atcagcggga
     8641 tcatgccagc catgttgacc atcaagggca aggttccctt aaccggcatc gacatgcgat
     8701 tgcctacccg gcgtccagga tacatcactg gaacgtttcg ccgcccctgt tgtacataga
     8761 cgatggcgaa aatgatcaaa gccatcatga tggcgatgaa gatgggcacg aagacccggg
     8821 tgcgctcatc ctgcaacaag cgcagcaggt tggtgggcac atccgacaca atacctgcaa
     8881 agatgattag ggaaagcccc tggttgcgaa taccgtactc tgagatgagc tcacccaacc
     8941 agatggcgaa catcgtgcca gcggtcatgc tgaggataat cgtgatcgac ggcaagaggt
     9001 ttgcgccgct gaagccaaag tcgctaatga tcggcacacc acctgtgaag gcattgaaca
     9061 aattaatctg tccaatcgcc tgtaaagcag ccatgggaac tgccagcata tacgtccact
     9121 tctccatcca cttctgcccc tcgcgtggat cttcttccat ccgcctctgc agagcaggga
     9181 taatgggaac cagaagctgc aggataatct gtgctgtgat gtagggatag acccccatcg
     9241 ccaagacgga gaaattggtc accgtgccac cggacaacat attgagcagg ctgaataagg
     9301 ttccacccgc accgccgccg gcgataatac tggcaatcgc ctcccggttc gctcctggta
     9361 caggcacatg cgctgccaag cggaatatgg ccaacaaacc gactgttacc agcagcttgc
     9421 ggcggatatc tttggcatac cagagaaagc gccaagcaga acgcttcatt tttggctcct
     9481 atgggtaaat accaaggtgt taattacctt catttcacag ctcaagaatt tcaacactac
     9541 caccggccgc ttcgatcttg gcttgggcac ccttgctgac tcggtgagcg ctcaccttga
     9601 gggcaacact cacctcacca cgtccgagga tgactaccgg gttttcgggt tcatgcagca
     9661 aacgagctgt ctccaacgtt tccggggtta cttcgctatc tgcgggaaaa tctgcgagct
     9721 gatccaaatt aacttcgttg tactcctggc ggaacagggg agtgaaaccc tgaccacgga
     9781 caaatggcag acggcggtag aagggtagat taccaccctg gtggtaaagg cgtacaccgc
     9841 ctccggcgcg cgcaccttga cccttgacac cccgtccggc attttttccc tggccggcgg
     9901 cggtgccgcg gccgacgcgc ttacgcgatt tttttgatcc tgggttcggc tgtaaatcgt
     9961 gcagtttcat tttattattc cacttcttcc acatctacta ggtggctcac ttttgcgatc
    10021 atgccacgca ccgaggccga atcgatatgt tccacagtcg cgtgcatgcg gtgaagtccc
    10081 agtgcgcgca cggtatcttt ctggcgctga gtgtagccga tgggactttt gaccagcgtg
    10141 acacgcagtc tcttttcaac aacctttgct ttcctagcca ttcttcctcc gctcccagaa
    10201 cggggtgacg tccttcaccg cctttccacg gaaggcagcc acttcttgag gcgatttgag
    10261 ctgttccaat gcgtttatgg tagcgtagac cacgttcagc acattgctgc tgcccaaaga
    10321 tttcgtcagg atatccgata cgccaactgc ctcgagcact gcgcgtacgc cgccacccgc
    10381 gatgacgcct gtacctgggg aggctggttt tagcaagact ttcgaaccag ccataatccc
    10441 catcacttca tgggggatgg tcgtgccaaa catgttcacc ttttgcatat tgcggcgcgc
    10501 cttatcggta gctttgcgca tggcatctgg caccgcattg gccttaccaa taccgacacc
    10561 aatgctcccg cgattgtcac ccacaaccac cgttacgcga aatgaaaagc gcctaccacc
    10621 cttaaccact ttggcaaccc gggcgatttc aacgacacgc tcgtcgagtt ctaattgatc
    10681 aaattcgtta tagtcggtca tatacttctc cgtgcagctg gctttaagcc cctgctaaaa
    10741 ttccattccg ccttcgcgag cgccatccgc aagcgcttta acccggccaa tgtagcgata
    10801 accgccccgg tcgaagacaa ctattgagat gcctttttcc ttggcgcgtt ctgccaatgc
    10861 ctttccgacc aggtgagcct gctcgctctt tttctttccc ttgagtgtat cgcgcaagtg
    10921 gcgatcaaca ctggaagcag agacaagcgt atgaccagcc tcgtcatcga ttacctgggc
    10981 gtagatctcg gccaggctgc gaaatacatt caagcggggt ttttctggcg tcccgctaac
    11041 atttttacgg acgcgtcgat gccgtcgcaa ccgtgctact gaacgtgatt taatcgtacc
    11101 catagattac accttgcctg ccttaccagc tttacggcga atcctctcgt caaggtattt
    11161 gatgccttta cccttatatg gttcaggcgg gcgcaccatt cggatattgg cggcaacctg
    11221 tccaaccatt tctttatcta cacccatcac cttgatttgt cgagtgcggg cgtccacttc
    11281 aaaagaaata ccaccgggcg gctccatctt gaccgaatga gagtagccaa cgtaaagaac
    11341 caaattttct ccatccaact cagcgcggta acccacgccg tttacttcaa ggattttctc
    11401 aaatccatca ctgacgccca caaccatatt gttgatcagc gcccgtgtca tgccatgcag
    11461 ggcgcgatgg cggggttcgt cgctagggcg cttcacgacg atctttccgt cctcgaccga
    11521 aatgatgata tcggccggga aggtgcgcgc caattcgccc ttgggacctt tcacccgcac
    11581 gttggagccc ttaattttca catctacccc attgggtaca tcgaccggtg cacgaccaat
    11641 tctcgacaca gatacctcct ttaccagacc ttacaaagga tctcgccgcc aacccctaat
    11701 tggcgagcac gctggccggt catcacaccc ttgggtgttg aaataatggc aacgcccatt
    11761 ccagataata cccaagggat ttcccgtttg ttggtataaa cccgtcggcc tggacggcta
    11821 atccgttcca gtccagtaat tacaggtttt cgttcgcgcc gctctccaat atactttaag
    11881 cggatgcgta agactttttg ccctacggtt ttgccatcta ccacttcgaa ggaagtgata
    11941 aatccctcat ctttgaggat tttggcgatc gccactttga gcttggagct gggtaaagcc
    12001 accatctggt gacctaccat gaccgcattg cgaatgcggg tcaacatgtc tgcgatagga
    12061 tcattcacac tcattctcaa accctccaac cggctaccag gatgacttca ctacgccagg
    12121 gattttgcct tctaatgcca actcacggaa gcaaatgcgg cataaaccaa accggcgcag
    12181 atacccgcgc ggtcgcccac actgtttgca gcgatttcgg acacgggtag ggtacttccg
    12241 cctggtttca cggatcatca tacacttttt ggccatcgcc taaccttcct tcttgaacgg
    12301 catgcccagc aattgcagca attgccggcc ttcttcatca ttgcgaccag tcgtcacaat
    12361 actaatttcc atgccacgta atttgtcgat cttgtcgtaa tcgatctctg gaaataccaa
    12421 ctgctctcgc aatcccaagg tgtagttgcc gcgcccgtcg aacgcattcg gcgagatgcc
    12481 gcggaaatca cgcacgcgtg gcaaagcaac gttcatcaag cgatccaaga atgcccacat
    12541 acggtcgccg cgcaacgtga ctttcacgcc gatttgccgg ccttcacgca acttaaagtt
    12601 cgcaatgctc ttgcgggctt tagtcacgat gggtttttgg ccggtcacct gggtgacatc
    12661 cgccacggtg gcgtccagtg ctttggcatt atccaaggct tcgccgactc ctacattaac
    12721 aacaatcttt tggatgcgcg gcgcctgcat ggtattttcc agatgaagcg acttcatcaa
    12781 tgccggcacg atctcttctt gatacctttc tttcaatatg tgtcccatca tttcgctccc
    12841 agataggcca tcttatacat cgatcagccc atggcaatgt ttacaaacac gtaaagattc
    12901 atcgccttca cgctgtaaac ccacacgggt tggctcgcta cacttcgggc aaaccaacat
    12961 cacgttggat atatggatcg gcccttcaaa ctcaatgatg cccggagaca ggttgcgccg
    13021 accctgggtc tggacctgcc gttggtgctt tttacgtata ttaatgcctt gcacaaccac
    13081 acgtccatca tcaggattta cgcggatcac ctcaccgcgc ttacctttat cttcaaagcg
    13141 accattgatc acttcaacca tatcgccttt gcgaattttc tttctcacaa tacgctctcc
    13201 tctccgcaga gtccttacag tacctcaggg gctagcgaga cgattttcat gaatcccttc
    13261 tcgcgcagtt cacgggcaac aggtccgaag atgcgggtac ctttaggatt tctgccatcc
    13321 tgatccagga tgaccgcggc attatcatcg aagcggatat aggacccgtc ttctcgccgc
    13381 cactccttcg cgcagcggac aacaactgct cgcacaatat cactcttctt caccgagccc
    13441 tgcggggtag ctgccttgac cgtagctacc accacgtcgc ccactcgggc ataacggcgg
    13501 gtagacccac ccatcacgtg gatgaccagc agctcgcgtg cgccggtatt atcggcaacc
    13561 ttcaagcgag attcctgctg gatcatggca cattctccaa aaggcccacc tcgggctcgt
    13621 cagcaccgat tgcgcggcgc agtatctctt caacaaccca atgcttgtgg cgagaaatcg
    13681 gtcggctctc cacaatacgt acatgatctc caacctggca acccaattca tcgtgggcca
    13741 taatccgctt gctggaatcg actacttttt tatacagtcg gtggcggtag gtacgggaaa
    13801 tttccacaac gactgtttta tccatcttgt cactggtaac cacaccagtt aagcgacggc
    13861 gagtgttcat gcttcacctt cttcctgggc tgtaaactgg cgctcgttaa gtattgtcat
    13921 taggcgcgca accgacttgc gagtgtagcg aagacgggtg taatcattca gaccgcccat
    13981 ggccagttga aagcgcagct tcattaattc ttctttcgca tcgtccaggc gagcctggat
    14041 atcctcagtg gaaagagcgc gtatttcctc tggtttcatg cctcgcctcc tcctgtctct
    14101 tcatagcggc caataatttt agttttgatg gccagcttat actgggctag gcgcaatgcc
    14161 tcttgagcca catctggggt caaaccacca cccacttcga acatgacccg gcctggtttc
    14221 acgaccgcaa cccaatattc cacattgcct ttacctttac ccattcgcgt ttccgctgct
    14281 ttgcgagtta ccggcttatc agggaaaata cggatccata cttttccacg gcgtcgcaga
    14341 gcgcgcacaa tcgcacgtcg ggcagcttca atctggcgtg cgctgaccca gcccggttcc
    14401 atagcctgca ggccaaattc tccaaaagag atttgcgcac cacgttgagc tttgcccctc
    14461 attcgaccgc gcatctgctt gcgccacttt acacgctttg gcattaacat gatcgcacct
    14521 tattcactaa cgtatacacc ttcagtcgac tcgagcttat cttccatctc gggcagtact
    14581 tcgcccttat agatccagac tttaaccccg atccgaccgt aggtggtatt ggcttcagct
    14641 ttggaaaagt caatgtcggc ccgtaatgtc tggcgcggta cctgaccctc gcgcatattt
    14701 accgcccgag ccatttcagc accgcttaac cgaccagaga cctccaaacg aatcccctca
    14761 gctccctggc ggatcgcctg ctggatggcg cgtttcatgg cgcgttggta gctaatccgg
    14821 cgctcaagct gatctgcgat attacgtgcc accaaatagg catcaaggtc aggcgatttg
    14881 atctctttga tctccaaatc aatctttttc ccgaccagaa cttctaggtt gctacggatt
    14941 ttcttcacgt tctcgccctt acgcccgatc aggatacccg gctttgccgt atgcacgagt
    15001 accttaacct ttccagggaa gcgctcgacc tcaattcgcg atactcccgc acgatcagcc
    15061 tcttgacgaa ctaattcccg caaggataaa tcctggtgca gctggttgac gtaatcctcg
    15121 ccttcggcat accagcgacc ttcccaagtc ttgttgatct tcaatcgaaa accggttgga
    15181 tgaactttac gacccattat gtctccttat gaattcgcac cagaaaacta ttcccgttct
    15241 cgcaggatca cagtgatatg agaggaacgt cttagccaag gtttaaaccg tccccgagcg
    15301 ccaaaacgcc gccatttgcg ggtaggagct tcgtttgcat agatactgta cacaaacaga
    15361 tcgtctcggc tcaccccaaa gttttcttct gcattggcta ctgccgaggc cagcgccttt
    15421 gataccggaa cagcagcagc gttggtaata aatttgagtg tagataacgc ctcgctaacg
    15481 ttcttcccac gcaccaagtc aattaccaat cggacttttt gcgcagaatg agggataaag
    15541 cgtgcatggg cgctgatatc tgtctccatg gcttaactcc ctttcttgat tttcttctcg
    15601 gcgagatggc cacggaaggt gcgcgtgggt gcgaactcgc ccaatcggtg acctaccatg
    15661 ttctcggtga tataaatcgg cacgtgacgg cgaccatcgt gcacagccac tgtgtgcccc
    15721 accatctggg gaaagatcgt gctggcgcga ctccaggtac gaatgacttt cttatcacca
    15781 cgctcgttca tgacttcaac tttcttcagc agttttgggt caataaatgg cccttttttc
    15841 agagaacgtc ccataatcta gtctcctggt cttaccggcg tttcttccct cggcgccgca
    15901 cgatgtactg gtctgtcttt ttattgcggc gggtcttgta acccagggta ggcttacccc
    15961 acgggctctt aggacccggc ataccgattg gctggcgccc ctcaccgcct ccgtgcgggt
    16021 gatcgcgcgg gctcatggcg gagcctcgta cagctggacg aatgcctttg tggcgtttgc
    16081 gccctgcttt acccagctta atgttgccat gatccaggtt acccacctgg ccaatgcttg
    16141 catagcactc ctggcgcacc aagcgcacct cgccagacgg catacgcact tgagcataat
    16201 cgccttcttt agccagcaat tgggctgctc cgccggcagc gcgcaccagt tgcccacctc
    16261 ggcctacttt cagttcaata ttatgtacca tcgtgcccac cggaatattg gcaatgggta
    16321 ggctgttacc gggacggatc tcagcacctg ggcctgctaa cacggtatcg ccaactttaa
    16381 gatccaccgg ggcgatgata tatcgttttt cgccatctac gtaattcaag agagccaagc
    16441 gagctgtgcg attgggatca tactcaatcg cagccacctt tgcgggcacg ccgcgcttat
    16501 cgcgcttgaa atcaacaatc cgaatatgcc gcttatgacc gccgccgcga tgccgcactg
    16561 tgatacgacc atacaggttg cgcccgcctg acttacgcag cggtttaagc aacgagcgct
    16621 caggcgactt cttggttatt tcttcaaatg tagatccggt catcccccgc tgacccgggg
    16681 tcgtaggttt gtactttttt acagccatta cttcacccct tcaaagatgt ctatcgagtc
    16741 ttcaggagcg agggtcacaa tggctttctt gtaagcgccg cggcgaacaa gcacgcgccg
    16801 actacggatt cgccgagtgc gccttgctgg cacattaacg atattgaccc gtaccacatc
    16861 cacatcgaag agtgtctcca cagcatcctt caccatgtgt ttggtggcgt ccgaagacac
    16921 ttcaaacaca tactggtgta agtcgctgtt tagataatta gacttttcgg tcacaatcgg
    16981 gcgacgcaat acatcataaa ttgaggtcat ctcaccctcc tacctatcca agaaatgaga
    17041 ccaaaacatc cagcaccttg agcggcatga gtactttatc gtagcccagc aagtcgcgga
    17101 tgtttagata attggcttgc agcatcttgg tgtccggcag attattggtt gagcggatta
    17161 ccatatcata accagtggtg ctctctggga tgagaatcaa cacgctggcg tcacccacca
    17221 gttgatcaag agcttgagcc atcaagcgag tttttggctc agacacgctc agctcatcta
    17281 ggacaacaat cgagctttca gcagccttca ccgaaagggc agagcgcagg gcagccctac
    17341 gcattttgcg gggcatctgc atgctgtaat cccttggctt gggagtatga atcttgccac
    17401 cgccaaccca ttgagcagct cgcgtcgatc cctggcgggc gcggccggtg cctttttggc
    17461 gccaaggctt gcgaccaccg ccggatacct cgctgcgacc tttcgtcttg tgtgtcccca
    17521 ggcgcgcatt tgccatctga cggacgtacg cctggtgcat caaatccaca tttaccgggg
    17581 cttcaaagat cagcgacggc aattctaccg tgctgacttt ctgcccttcc atattcaaga
    17641 catcaacttc catgattcca tcgctccact atatgacaac ccggttcgag gtgctatcat
    17701 gcaggctatc gcttacgcgc ctctttgatc atcacaatac cgcctttagg gccaggaatg
    17761 gcaccccgca cgcccatcag gttgcgctca ctgtcaatca gcaccacttt gagattctgc
    17821 acagttaccc gttcattacc catatgacct gctccccgcg atcctttata gacgcggcct
    17881 ggcgtggtgc ccgatccgcg cgatcccgga gctcggtggc gatccgactg gccatgggtc
    17941 tttgggccac cccgaaaatg gtggcgcttc accgaacctg caaaaccttt acccttgctg
    18001 gtaccgatga cgtccacatt ctcaccaacg ttgaaaactg catcgacggt cagcgtgtcg
    18061 ccttctttga cttccggacc tttgtggcga aactcgcgta aaaagcgcaa cggaggcgta
    18121 ttgttgcgct tgagatgccc aagttggcca gaagttaggc gcttctgctt gacctccaca
    18181 aatcccagct gcactgccga ataaccatct acctccggca accgaacctg ggtgacataa
    18241 caaggcccag cttcgattaa ggtaaccggg atggcgacac cggcgtcatc gaaaatctgg
    18301 gtcatgccga ttttcttgcc aatcagccct ttcaacatct ttttttctcc taaatatcaa
    18361 gttttgggac tgtcagcctg ggctgggctg catcatcccc aacggcctcg cagttagccg
    18421 ggctgccgca gatcaatggg atggttgggt ctgcgcacag gcctgctcac gcgatgcctt
    18481 ttcgagataa cgtttagggg tgagaaagac ccagtcccta tccattatct ttattacaac
    18541 ttgttaaatc tttatctcaa tatcaacacc cgccggcaga tttaaccgca tgagcatatc
    18601 gatggtctta gagtctggat caagaacatc gatcagccgg ttatgggttc tcacttcaaa
    18661 gtgctcgtgg gaatctttat caataaatgt cgaccggcgc actgtgaaac gctcaatacg
    18721 ggttggtaga ggcactggcc ccaccacgcg agcaccagtg cgctcagcgg tttcaacaat
    18781 acgcttggca gactgatcta gcacgcgatg atcgtatgct ttgagacgaa ttcgaatctt
    18841 ctgctttgcc atgatcatcc acctaatcca gaatcttagt aataacgccg gcgccgacgg
    18901 tcaagccacc ttcacggatg gcgaacttgg agccctgctc cagcgccacc ggcacgatca
    18961 actccacttc caagttgaca ttgtcacccg gcatgaccat ctccacgcct tccggcaggg
    19021 tgatattccc ggtcacatcc atggtgcgga tgtagaactg cggccggtac cccgtgaaga
    19081 acgccttgtg ccgcccgcct tcttccttgc gca
//
LOCUS       DBNGSX010000515         5855 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_606666, whole genome shotgun sequence.
ACCESSION   DBNGSX010000515 DBNGSX010000000
VERSION     DBNGSX010000515.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5855)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_606666"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="AC16CB_15380"
     CDS             complement(<1..879)
                     /locus_tag="AC16CB_15380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003579096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0008076 - voltage-gated potassium channel
                     complex [Evidence IEA];
                     GO_function: GO:0015459 - potassium channel regulator
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel beta subunit family protein"
                     /protein_id="HIR3343904.1"
                     /translation="MEYRFLGKSGLKISALSLGAWVTFGQQISDDVAFECMKLAYDAG
                     INFFDNAEAYAHGKAETTMGNVIKKAGWKRKDLVISTKIFWGGKGPNDRGLSRKHIFE
                     GLNASLQRLQMDYVDLVFCHRPDIYTPIEETVWAMNQAIQQGKALYWGTSEWSAYQIL
                     DAYRIARREHLIPPLMEQPEYNMFHRERVEKEYAPLYDEIGLGTTIFSPLASGLLTGK
                     YNQGLPPGTRANLKGYEWLKEWIESEESQKNIAKARQLTPLADELGCSMAQLALAWCL
                     KNQHVSTVITGASRPDQ"
     gene            complement(951..1760)
                     /locus_tag="AC16CB_15385"
     CDS             complement(951..1760)
                     /locus_tag="AC16CB_15385"
                     /inference="COORDINATES: protein motif:HMM:NF012673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR3343905.1"
                     /translation="MISTLQFATELACSTGKLLTEFYRPGGSEARLKADQSVVTEADL
                     AADRLVKQAIRDAYPQDLLLSEELHPSLSVGNQEAVWVVDPLDGTTNFSLGLHIWGVS
                     IARAVNGIPETGAIYFPLLDELYSAQIGTGAFWNGERIRVQPMDKEKPATFFACCGQT
                     HKLYNVSVPYKPRILGSAAYDFCALARGAAVLTFEATPKIWDLAAVWVLILEAGGVIE
                     SHHGPSPFPLTPGMNYTKRAFPTLAAATAKMVVKGRNMIKIKDELMLTNDG"
     gene            complement(1757..3736)
                     /locus_tag="AC16CB_15390"
     CDS             complement(1757..3736)
                     /locus_tag="AC16CB_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3343906.1"
                     /translation="MSITADLILTNAIVLTMDEKFAHYEPGAVVVNGDSITAVGPDAE
                     IRAAYSAKETLDCGGKVLMPGFVNAHTHVPMTLLRGLADDLRLDVWLMGYMMPVEREF
                     VSPDFVRLGTKIACVELIRSGVTSFADMYYFEEEVARATAEAGLRAVCSQTVMKYPTP
                     DAQFYEESLASARDFLEKWKGHPLIVPSVAPHAPYTCTPDILNATAQLAVEFDVPLHT
                     HLSETAGEVENSRDEHGMPVIPYVKKQNLFDAKVLAAHCVHVDEGEIYTLLHHHAGIA
                     HNPSSNLKLASGFAPVSKMLEAGLNVGIGTDGPASNNDLDMFEEVRLAAFLAKAHSND
                     PTVLPASSALAMATRLGANALHIGSLTGSLEPGKRADFILVDLSPLHNAPRFRRDPNG
                     LYAQLVYAGKSTDVTHVMVNGQWLMRDHQLTTLDESDLLTQAAEYASKIDYFLIQREQ
                     SVLSKLIAIGGATEAESFEVQAKVRISDTSSVHQALEKPEIEILHHRHYREYDTYFSF
                     EDPKQGHLRYREDEFIDSKGEVSNVRYRLTLLGPAREEHFPSDVLLSRSRFIAPATHS
                     LRFYREYFKPTSETAIQKDRLRWRILFRGTEFYINLDFVNTPDLGTFLEVKSRTWSRR
                     DAEFKAELATDIIIFLGALPEKTVAQDYIEIVEDR"
     gene            complement(3753..4895)
                     /locus_tag="AC16CB_15395"
     CDS             complement(3753..4895)
                     /locus_tag="AC16CB_15395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HIR3343907.1"
                     /translation="MPCAEIITIGTEILLGDIVDTNAAYLARALRDAGIDLYRKTTVG
                     DNVRRIAQAIQQSLERCDIILTTGGLGPTVDDPTREAVALAFGVETEFLPELWEQIQA
                     RFRRYNRQPTENNKRQAYVPRGATPIENPVGTAPAFAVEKGDRVVISLPGVPREMEYL
                     VQNAVLPYIKQRFNLQGIIKSRVIHTAGVGESQIDDLIGDLEALSNPTVGLAAHSGQV
                     DVRITAKAASESEADALIQPIEVTLRQRLGKWVYGADQETLEAAALENLQRKGWSLAV
                     VEAGLAGNLIRRLASAQGPFLGGQVLPHSPTPEELTSLTDAYRLSCSAEIGLGVAIYP
                     AGDRQDVYLTLVSPDGIQEITRPYGGPKEYAPRWALHHSLDVIRNL"
     gene            complement(4955..>5855)
                     /locus_tag="AC16CB_15400"
     CDS             complement(4955..>5855)
                     /locus_tag="AC16CB_15400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3343908.1"
                     /translation="DYRQCGYLFVLTQPADVEVFKRNVALQNQLGAGTIWLDGDEIRR
                     RLPLMHMEDVIAGTFNPKDGLVDPNGVVMGYIRSAQRRGVQALTGMEVIGVKVVGGQV
                     EGVETSAGVIATRVVINAAGPWAGLVGRMAGVDIPITPIRRQMFTTTPIPEIPPDFPF
                     VIDFAQSLYFHRESQGLLIGMSNPNEKPGFDQNVDPEFELVNLESATARLPLLEHAGL
                     ASHWAGLYEVTPDAHPIYGKTPVEGFYTCTGFSGHGFMHGPISGKLMAEILLDGAAHS
                     VDISMLDLARFEEGRLIREYNVV"
BASE COUNT         1350 a         1552 c         1537 g         1416 t
ORIGIN      
        1 ctggtctggg cgagatgcac ctgtgattac cgtactcaca tgttgattct tgagacacca
       61 tgctaaggct agttgtgcca tgctgcagcc cagctcatca gcgagtggtg ttaactgccg
      121 agctttagca atgttttttt ggctttcttc gctttcaatc cactctttta gccattcata
      181 tcccttcagg ttagcccgtg ttccaggggg aagaccttgg ttgtacttcc cagtaagcag
      241 cccgctagct aatgggctga agatggtggt ccccaagcct atctcatcgt atagcggagc
      301 atactctttc tctacgcgct cgcgatggaa catgttgtat tcgggctgct ccatcagcgg
      361 aggtatcaaa tgctcgcggc gggcgattcg ataggcatcc aagatctggt atgcgctcca
      421 ctcgctggta ccccaatata gagccttccc ctgttggata gcctggttca tcgcccagac
      481 tgtctcttca atgggggtgt agatgtcggg tcggtggcaa aaaaccaggt caacgtaatc
      541 catctggagc ctttgtagcg aagcgttcag gccttcgaag atgtgtttgc gagataaacc
      601 tctgtcattc ggtcctttgc ctccccaaaa gattttggtt gagataacga gatctttgcg
      661 tttccaacca gcttttttta tcacattccc catcgtggtc tcagccttgc catgggcata
      721 ggcttcggca ttatcgaaga aattgatgcc ggcatcatag gccagcttca tacactcaaa
      781 ggccacgtcg tcgctgatct gttgtccaaa ggtgacccag gctcccaatg agagagccga
      841 aatttttagt cctgactttc ccagaaagcg gtattccata ttagcattct ccaagtgatg
      901 tgaaatttac tagtttctag aactgatgta ttcaccgctg atggacggta tcatccgtcg
      961 tttgtcaaca tcagttcgtc tttgatttta atcatatttc tacccttcac caccatttta
     1021 gcagttgctg ctgccagggt gggaaaagcc cgtttggtgt aattcatgcc aggcgtcaat
     1081 ggaaacggtg aaggaccatg atgagattca atcactccac cggcttcaag tatcagcacc
     1141 cagactgctg ccaggtccca aatcttaggt gtggcctcaa acgttagcac ggcggcaccg
     1201 cgcgctaatg cacaaaagtc gtaagcagct gagcctaata tgcgcggttt atagggaaca
     1261 ctaacgttgt agagcttatg ggtttgcccg cagcaagcga agaatgtagc tggtttttct
     1321 ttatccattg gttgaacccg gatgcgctcc ccattccaga aagctcccgt gccaatttgg
     1381 gcggaataaa gctcatctaa caaagggaag taaatggccc cggtttcagg tataccgttc
     1441 actgcgcgtg cgatggaaac cccccaaata tgcaacccca agctaaagtt ggttgtaccg
     1501 tccagcggat caaccaccca gacagcctct tggtttccaa cagacaacga tgggtggagt
     1561 tcttccgata agagcaaatc ctgtggataa gcgtctcgga tagcctgttt caccaggcgg
     1621 tcggctgcca gatcggcttc cgttaccaca gactggtcag ccttcaggcg tgcctctgat
     1681 ccaccaggcc ggtaaaattc tgttagcaac ttcccggttg agcaggcgag ctctgtagcg
     1741 aactggagtg tgctgatcat cgatcttcga cgatttcgat ataatcctgc gctacagttt
     1801 tttcgggcaa tgctcccagg aatatgatta tatcagtcgc aagctcggct ttaaattcgg
     1861 catcccgccg gctccacgtg cggctcttta cttccagaaa ggtgcctaga tcaggggtgt
     1921 tgacaaaatc gagattaata tagaattcgg tgcctcggaa taagatcctc cagcgcaagc
     1981 ggtctttttg gattgccgtc tcagaagtcg gcttgaaata ctcccggtag aaacgcaagc
     2041 tatgtgtggc tggcgcgatg aatcggctgc gcgagagcaa tacgtcgctg ggaaaatgct
     2101 cttcgcgcgc agggcctagc agagtcagac ggtagcgcac attgctgact tcacctttag
     2161 agtcgataaa ctcatcctcg cggtagcgaa gatgcccttg tttgggatct tcaaaggaga
     2221 aataggtatc gtattctcgg taatggcggt ggtgcaggat ctcgatctcg ggcttttcca
     2281 gtgcttgatg tactgaggat gtatcggaaa ttcgcacttt agcctgcacc tcaaagcttt
     2341 ctgcctcggt cgctccaccg atggcgatga gcttggagag caccgattgc tccctctgaa
     2401 taagaaaata atcaatttta gaggcatatt ctgcagcttg cgtcagcaga tcggactcat
     2461 cgagggtagt cagctggtga tcgcgcatca gccattgacc attgaccatt acatgggtga
     2521 catcggtcga cttgccggca taaaccaact gtgcgtacag tccgttgggg tcgcggcgaa
     2581 agcggggtgc attatgcagt ggggagagat ccaccaggat aaaatcggcg cgtttgccag
     2641 gttccagcga gccggtcagg ctgcctatgt gaagggcatt tgcgcccagg cgtgtagcca
     2701 ttgccagggc agacgaggcg ggcaacaccg tgggatcgtt tgagtgagct ttggccaaga
     2761 aagctgccag gcgcacttct tcaaacatgt ccaggtcatt attggaggct ggcccgtcgg
     2821 tgcctatgcc gacatttaga ccggcttcca acattttgct caccggcgca aaacctgagg
     2881 ctaacttgag gttagaagat ggattatggg ctatacccgc atgatggtgt agtagagtgt
     2941 aaatctcccc ctcatcaacg tgaacgcaat gagccgcaag cactttggcg tcgaacaggt
     3001 tctgtttctt gacatagggg atgactggca tgccgtgctc atcgcgtgag ttttccactt
     3061 cgccggcggt ctcagacaaa tgagtgtgca agggtacatc gaactcaaca gccagctggg
     3121 cagtggcgtt caggatgtcg ggtgtgcatg tatacggggc gtgcggtgct accgaaggga
     3181 caatgagtgg atgacctttc cacttttcaa ggaaatcacg agcagaggct agggattctt
     3241 catagaattg agcatctggt gtgggatatt tcatcacagt ttgtgaacaa actgcgcgca
     3301 gcccagcttc ggcggttgcc cgggcgactt cctcttcgaa ataatacatg tcagcgaagc
     3361 tggtcacacc cgagcgtatc agctcgacac aggcgatctt ggtgccaaga cggacaaaat
     3421 ccggggaaac gaattcgcgt tcaaccggca tcatgtaccc cataagccaa acatctaatc
     3481 gcagatcatc tgccaggccg cgcaacaggg tcatgggaac gtgggtatgg gcgttgacaa
     3541 agcctggcat cagcacttta ccgccgcaat ccagcgtctc tttggcggag taagccgccc
     3601 ggatttccgc atcaggccca acagcggtaa tgctgtcacc gttgactacc actgcaccgg
     3661 gttcatagtg agcaaatttt tcgtccatgg tcaggacgat ggcatttgtg aggataagat
     3721 ctgcggttat ggacacaaaa tcacctttct agtcaaagat tacgaattac atccaggctg
     3781 tgatgcagtg cccagcgcgg ggcatattcc ttcgggccgc cgtatgggcg ggttatctcc
     3841 tggattccat ctggggaaac cagggttagg tacacgtctt gacggtcgcc agctgggtag
     3901 atcgcaacac ccaacccaat ctcggcgctg caagacaggc ggtaggcatc cgtcaaggag
     3961 gttaattcct cgggtgttgg ggagtgtggc aacacctggc ctcctaggaa aggcccttga
     4021 gctgatgcca acctgcggat taaattgcca gccagccccg cctcaaccac tgccaggctc
     4081 caacctttac gctggagatt ttccagggca gctgcttcca gggtctcttg atctgcacca
     4141 tatacccact tccctaagcg ctgccgcaga gtaacttcaa tgggttggat gagagcatcg
     4201 gcctcacttt ctgatgcagc ttttgcggtg atgcgcacat ccacttgtcc agagtgggct
     4261 gccaagccca cggtggggtt acttagcgct tctagatcgc caatcagatc gtcgatctga
     4321 gactcaccta cacccgcagt gtggataact cttgacttaa tgataccctg gagattgaag
     4381 cgctgcttga tgtaaggcaa gacagcgttc tgaaccagat actccatctc ccgaggcacg
     4441 cccggcagcg agataaccac ccgatctccc ttctctactg cgaaagccgg cgctgtccct
     4501 accggatttt caattggtgt ggcgcctctg ggtacataag cctggcgttt gttattctcg
     4561 gttggctggc ggttgtaacg ccggaagcga gcctggatct gctcccataa ttcggggagg
     4621 aactcagtct ccacgccaaa tgccagcgca accgcctccc gggtcggatc atccacggtg
     4681 ggtcccaacc cccccgtggt taggatgata tcacagcgtt ccagggattg ttgaatagcc
     4741 tgggcgatac gtcgcacgtt gtcgccaacc gtcgtcttgc ggtacaagtc aataccggca
     4801 tcacgcaggg cacgagcgag atacgcagcg ttggtatcta ctatgtcgcc aagtaatatt
     4861 tcggtcccaa tggtgatgat ctctgcacag ggcatattct aactgcggct aggcttaggg
     4921 tctacatgaa gttgttcggc ttatggatca gccttcaaac tacattatat tcgcgaatca
     4981 agcgaccttc ttcgaaacgg gctaggtcga gcatagagat atctacacta tgggctgcac
     5041 cgtccagcag aatctcggcc attagcttgc cagaaatcgg cccgtgcata aagccgtgcc
     5101 ctgaaaaccc ggtgcatgtg taaaaaccct ctacgggtgt tttgccatag atcggatgtg
     5161 catctggggt tacctcatat aaccccgccc agtgcgatgc caatccagca tgctccagca
     5221 ggggaaggcg agcggtggca ctttcaagat tcaccagctc aaactctgga tcgacattct
     5281 ggtcgaaacc tggcttttca ttggggttgg acatgccaat cagcaacccc tgactttcgc
     5341 ggtgaaaata caatgattgg gcaaaatcaa ttacaaaggg gaaatcggga gggatttctg
     5401 ggatgggggt tgtggtgaac atttgccgtc taataggggt gatcggaata tccacaccag
     5461 ccatgcgacc caccaacccg gcccaagggc cagccgcgtt gattaccacg cgtgtggcga
     5521 tgaccccagc ggaagtctcc accccttcaa cctggcctcc gactacctta actccaatca
     5581 cttccatgcc ggtcaacgcc tgcacacccc tgcgctgtgc ggagcggata tagcccatga
     5641 ccacaccgtt gggatcaacc aaaccatcct tggggttaaa agtacccgca atcacatctt
     5701 ccatgtgcat gagaggcaaa cggcggcgga tctcatcacc atccagccag attgttcccg
     5761 ctcctagctg gttttgcagc gctacgttac gtttgaagac ctccacatct gcaggctgag
     5821 tgagcacaaa tagataccca cactggcggt aatca
//
LOCUS       DBNGSX010000516         2739 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_190767, whole genome shotgun sequence.
ACCESSION   DBNGSX010000516 DBNGSX010000000
VERSION     DBNGSX010000516.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_190767"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /gene="fmt"
                     /locus_tag="AC16CB_15405"
     CDS             <1..959
                     /gene="fmt"
                     /locus_tag="AC16CB_15405"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR3343909.1"
                     /translation="EASPCLVTRDTGLPMKPHLVFMGSPKFALPTLSALAENYSIQGV
                     VTQPDRPAGRGKELTPPAIKSFAIDLGLPVIQPEKIKDPVAMQQLANWAPDLIVVVAY
                     GQILRQTVLDLPRNGCINVHASLLPRWRGAAPIQAAILAGDEWAGVTIMRMDSGVDTG
                     PILSQRGIPILCEDTAGSLSERLAEMGAELLIETLPAYLMGELQPRPQSESGVTYAPM
                     LKKGDGQLDFTQSALDLARRVRAFNPWPGATTQWKGQPLRIHSAHAIEDYLSPPGQRL
                     VYGPKPAIGTGQGTLVLDEVQPAGKKAMPGDVFLRGARDWEE"
     gene            956..2047
                     /locus_tag="AC16CB_15410"
     CDS             956..2047
                     /locus_tag="AC16CB_15410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020490126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR3343910.1"
                     /translation="MNILIFGAGAIGTYIGGSLILRGDRITFIEQPQAVEEITRRGLY
                     LDLSAAQRKPAPVDTQPSITDLHPNIVLPPSYFYCVRSLEEALKLGPYDLAIFALKSF
                     DTQPVIESLQPFLSDLPTFLCLQNGVENEVALANALGPDRVIAGTVTSAVSRRNIGEI
                     ILERLRGIGVALGHPISRRLVYILNEAGLNAQVYTSSAEMKWSKLLTNLLANATSAIL
                     DMTPAEVFAHPGLYRLEIDQLVEALRVMDAQNLRVVNLPGTPVRLLALGTRLPIFLSK
                     PLMQRSVGKGRGAKMPSFHIDLHSGRGKSEVDFLNGAVARFGKRLGVPTPVNHTLNET
                     LLALTEGKTPLEFYARKPQVLLDSMGERE"
     gene            complement(2103..>2739)
                     /gene="deoA"
                     /locus_tag="AC16CB_15415"
     CDS             complement(2103..>2739)
                     /gene="deoA"
                     /locus_tag="AC16CB_15415"
                     /inference="COORDINATES: protein motif:HMM:NF019448.7"
                     /note="Catalyzes the reversible phosphorolysis of
                     thymidine, deoxyuridine and their analogues to their
                     respective bases and 2-deoxyribose 1-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HIR3343911.1"
                     /translation="ARFVGRRAVALLSDMNQPLGNAVGNALELKEAIQSLHGSGPADF
                     LEHCLEVAGYMLAIGGKAADQNEGMQLAHAALDDGRGWEFFKRLVAAQGGDVAVIENP
                     DRLPKARFVKEVASPRSGYLSEIHARIVGETVVSLGGGRAKKGDPIDHAVGAIIHHKV
                     GDHVEAGEPLFTIHANDQARLEDAHQRLLQAHSWSDSPVEPLPLFYGVVQE"
BASE COUNT          617 a          797 c          703 g          622 t
ORIGIN      
        1 gagaagcttc cccatgtctg gttacccgag acactggttt acccatgaaa cctcacctgg
       61 ttttcatggg ctcgccaaaa ttcgcccttc ccacgttaag cgcgctagct gagaattatt
      121 ccatccaggg cgtggtcacc caaccagacc ggcctgccgg tcgcggcaag gaactcacac
      181 cgcctgcgat caaatccttt gccatagact tagggttacc cgtcatacag ccagagaaaa
      241 taaaagatcc agtagccatg cagcaattgg ctaactgggc tcctgacctc attgttgttg
      301 tagcctatgg acaaatcctg cgccagacag tgcttgatct gccgaggaat ggctgcatca
      361 atgtgcatgc ttccctgctg ccgcgctggc gcggggctgc ccccatccag gctgcgatcc
      421 tagccggcga cgagtgggct ggtgtcacga tcatgcgcat ggacagcggt gtggacaccg
      481 gccctattct cagccagcgc ggtatcccca ttctgtgcga agataccgcc ggctctctct
      541 ccgagcgact ggcggaaatg ggggcagagc tgctcatcga gaccttgcca gcatatctta
      601 tgggggagtt gcaaccgcgg ccccaatccg aatcgggggt cacctacgct cccatgttga
      661 aaaaagggga tggacaattg gatttcacgc aatctgcgct tgatctggcc cgaagagtaa
      721 gggcatttaa cccctggcca ggagctacca cccagtggaa aggacagcca cttcggattc
      781 atagcgctca cgcgattgaa gattaccttt ccccgcctgg tcaacgtctc gtctatgggc
      841 caaaacccgc catcggtaca ggacaaggca cgcttgtgct ggatgaagtg cagccagctg
      901 gcaaaaaggc tatgccaggc gacgttttcc tgcgcggcgc aagagattgg gaagaatgaa
      961 catcttaatc tttggcgctg gcgctatagg tacgtatatc ggcggtagct tgattttacg
     1021 cggcgaccgt atcaccttta tcgagcagcc ccaagccgtc gaggagatca cgcgtcgcgg
     1081 cctgtaccta gatctgtcgg ctgcccagcg aaagcctgcg cccgtcgata cccaaccatc
     1141 cattaccgat ctccacccca acattgtcct tccaccttcc tacttctact gtgtgcgatc
     1201 cttggaagag gcacttaaac taggaccata cgatcttgct atatttgccc ttaaatcttt
     1261 cgacacccag cctgtgatag aaagtttaca acccttcctg agcgacctgc ccacctttct
     1321 ttgcttgcaa aacggggtag agaatgaagt cgccctggca aatgccttgg ggcctgatcg
     1381 cgtgatcgcc gggacagtga caagcgccgt aagtcgccgc aatatcggcg agattatctt
     1441 ggagcggctg cgcggcattg gggtcgcgct ggggcaccca atctccagac ggttggtcta
     1501 tatcttgaac gaggctggat tgaatgcaca ggtttacaca tcctctgcag agatgaagtg
     1561 gtccaaactg ctcaccaacc ttctggcaaa tgccacctcg gccatcttgg atatgacccc
     1621 cgctgaagtt ttcgcacacc cgggtttata ccggctggag atagatcagt tagttgaggc
     1681 gctgcgggta atggatgccc agaatttgcg tgttgtaaac ttgcctggaa cgcctgtacg
     1741 gttgctggct ttaggtaccc gactaccgat ttttctctcc aagcctttga tgcaacgcag
     1801 tgttggcaag gggcgcggtg caaagatgcc gtcttttcac atcgatctac acagcgggcg
     1861 cggtaaaagc gaggtggact ttctcaacgg cgctgttgcc cgttttggca agcggctagg
     1921 cgtgccgacg cccgtaaacc ataccttgaa tgaaaccctg ctggcgctaa ccgaagggaa
     1981 gacaccattg gagttttacg cgcgcaaacc gcaggtgtta ttggatagca tgggggaaag
     2041 ggaatagaaa aggcacctaa cacccctacc atagagcttt tccccatatt agcgtcgcgc
     2101 ctttattcct gaactacacc ataaaataaa ggtaacggtt cgaccggact atcactccaa
     2161 gaatgcgcct gcagcaaacg ctgatgagcg tcctcaaggc gtgcttgatc attggcgtga
     2221 atggtgaaaa gcggttcccc tgcttccaca tggtctccaa ctttatgatg aatgatcgcg
     2281 cctacggcat gatcgatcgg gtcacccttt tttgctcttc cgccacccaa cgatacgacc
     2341 gtctccccca caatccgggc gtgaatctca ctgagatagc cggaacgcgg cgaagccact
     2401 tcttttacaa agcgagcctt tggcaaccgg tctggatttt ctattacagc aacatcgcca
     2461 ccttgtgcag ccactaacct tttgaagaat tcccaacctc ggccatcatc cagcgcagca
     2521 tgggctagct gcattccttc attctgatcc gctgcttttc ctccaattgc cagcatgtat
     2581 ccagcaacct ccaggcagtg ctcaaggaag tcggctggtc cgctaccatg caaggactgg
     2641 atggcttctt tcaattccaa tgcgttcccc acggcatttc ctaaaggctg gttcatgtcc
     2701 gagaggagcg ctaccgccct ccgcccaaca aagcgggcg
//
LOCUS       DBNGSX010000517         5217 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_191213, whole genome shotgun sequence.
ACCESSION   DBNGSX010000517 DBNGSX010000000
VERSION     DBNGSX010000517.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_191213"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="AC16CB_15420"
     CDS             complement(<1..638)
                     /locus_tag="AC16CB_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR3343912.1"
                     /translation="MKIDSPSSAEVAIIGAGPIGLELAAVLKRLGVDYLHLEARQIGY
                     TISWWPRQTYFFSTTERIEIAGIPLQNTAQQRATGEEYLAYLRSVVEQLDLQVSTYEP
                     VVGIQRTENGFQLITLPQTGERRYLCGKLVLAIGDMHAPNRLGIPGEDLPHISHYFDD
                     PHKYFRKRLLIVGGRNSAAEAALRCWRAGSQVSISYRREDFDPHVVKHWILP"
     gene            complement(705..1658)
                     /locus_tag="AC16CB_15425"
     CDS             complement(705..1658)
                     /locus_tag="AC16CB_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HIR3343913.1"
                     /translation="MKKIITIVAVFLISLAYGCQVNPVPTPQVGEAGLIVLAAESFLA
                     DIVQNVAGDRLTVETLLPAGLDPHAFEPTPKDVARIADSDILIVNGAGLEEWLENITE
                     AAGGERLVIEASAGLTPRYRSSGGACGASSQIAEGGHNGEGDPHFWLDPSLVIHYVEN
                     IRDGLSQVDPQGEDQYTLNADAYTARIMELDSWITSQVDQIPSDRRLLVTNHESLGYF
                     ADRYGFQLTGAILPSFSTAASPSAADLARLADCIRSTGAPAIFLETGANPQLAEQLAQ
                     ETGIRVVTELYSHSITPPNGNAPDYLSMMKYNTQTIVNALK"
     gene            complement(1856..2908)
                     /locus_tag="AC16CB_15430"
     CDS             complement(1856..2908)
                     /locus_tag="AC16CB_15430"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR3343914.1"
                     /translation="METLIREWGGETVIVHYDRPTGAWIFIAIHSTRLGPAVGGTRMK
                     PYASVQAALQDAMRLAAGMTYKFATPGMEFGGGKAVIAIPADFEAQSRPALLRRYGAL
                     IHQLGGLFSTGPDVGTSPADMDIIAETGAPYIFARTPAAGGAGDPGPFTARGVFAGIE
                     ETCEYLFGDMSLVGRRVLVQGAGDVGAKLINYLCDAGATVAYSEVDEGAIRKVRDDLG
                     LEFVPPEAVYTTECDIFAPCALGGVLNADTIPQLKCRAVVGGANNQFATQEDAERLRA
                     RGILYAPDYVVNVGGAMAITSMEMKGWSQERAEREVVESVRRALKQIFEVASKQGITT
                     DAAARQLAENRLSAGK"
     gene            3057..3503
                     /locus_tag="AC16CB_15435"
     CDS             3057..3503
                     /locus_tag="AC16CB_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343915.1"
                     /translation="MTTKTEIMDQKRKRYRRAHVIWFAVFFIAWIIRSALKLFELDLP
                     LLENMLLLILIFSVAVQAFYALKDNMLAIELRKDPQLKEAMNDELVQLNELKAWKAAF
                     FSLIGYIIFVALLSMFIDFNDLMSIFITALIIGFGTYNSAAFVLNR"
     gene            3508..3717
                     /locus_tag="AC16CB_15440"
     CDS             3508..3717
                     /locus_tag="AC16CB_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3343916.1"
                     /translation="MEEKKLRNTIRVQRAKKNLTQEQLAELVGVTRKTINTVENGIYI
                     PSTYLALKLSRALDVTVEELFQLSE"
     gene            complement(3841..5004)
                     /locus_tag="AC16CB_15445"
     CDS             complement(3841..5004)
                     /locus_tag="AC16CB_15445"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3343917.1"
                     /translation="MAFPYKETVDPESVNVDAGRLARVVDLFQNQQLTGSFPGGQLVF
                     SRNGKLVVNIAIGIARGFRPSEPIPPTQVHSQTPFPVLSAGKPLAAIAIAMLEDRGVL
                     DVKAPIAHVFPEFARHGKEQITTLDVLTHRSGILMPDFVKNTPLHGDIEAIREAIIEA
                     LPSYPRGTLAYHPHEYGWILNEIVLRVDGRSLPDFFREEIAAPLQLPALQFGLAGRDI
                     NTLAFSYWLGKQSFLVADVNVAEDFEWQNSEQFFNARNPATSLVSDAASLAAFYDFLL
                     SGGKTPAGKQLISEMAIQKYTSRHVLSWDRSLKTVIAMGRGFVVGTRFPSSFGWWSTN
                     RCFGHPGGFSCLAFGEYSKNISVAIVTNGNRGTIDFAKRFLPLADGLRKACMS"
BASE COUNT         1364 a         1383 c         1227 g         1243 t
ORIGIN      
        1 tccggcaaga tccagtgttt gactacatgc gggtcgaaat cctcccggcg atagcttatg
       61 ctgacctggc tgcctgctcg ccagcagcgt agggctgctt ctgcagccga gttgcgccct
      121 ccgactataa gcaagcgctt gcgaaagtac ttatgagggt catcaaagta atgggagata
      181 tgaggcaggt cttcaccggg aatccccagg cgatttggag catgcatatc gccaatagcc
      241 aggaccagct tgccgcataa ataacgccgc tcgcccgtct gcggtaaagt tatcaattgg
      301 aagccattct ctgtccgctg gatgcccacc acaggctcat aggtacttac ctgcaagtct
      361 agctgctcca ccacgctgcg caggtatgcc agatattctt cacccgtggc gcgctgctgg
      421 gcagtgtttt gcagggggat gccggcgatc tcgatgcgct ctgtggtgct gaagaaatac
      481 gtctggcgcg gccaccagga gatggtgtag ccgatctggc gggcttccag gtgcaggtaa
      541 tccacgccga ggcgcttcaa gactgccgcc agttccaacc caattggccc ggcaccgatg
      601 atagctacct cagcagaact cggcgagtcg attttcatac gatcccgaaa tcctccctct
      661 ataccccaaa ggatttgaaa accttgggga gtcttggcat tcacttattt tagggcgttg
      721 acaatggttt gcgtattgta cttcatcatc gagagataat ctggggcatt tccatttggc
      781 ggggtgatcg aatggctgta aagctcggtg acaacccgta ttcccgtctc ctgggcgagc
      841 tgttcagcta gctgtgggtt cgcacccgtc tccaagaaga tggctggcgc accggtcgag
      901 cgtatacaat ctgccaggcg cgctagatcc gcagcggatg gagatgccgc agtgctgaag
      961 ctgggcaaaa tagcaccagt aagctgaaaa ccatagcgat cggcaaagta gcccaaggat
     1021 tcgtggttgg tcaccagcaa gcgacgatct gacgggattt ggtcaacttg cgaggtaatc
     1081 caggaatcca gctccataat tcgggctgtg taggcatccg cgttcaaagt gtattggtct
     1141 tccccttggg gatcgacctg gctcaatcca tcgcggatgt tttccacgta atgaatgacc
     1201 aggcttggat ctaaccaaaa gtgtgggtca ccctcgccgt tatgtccacc ttcagcaatc
     1261 tgggatgaag cgccgcacgc ccccccagaa gatcgatacc tgggagttaa acctgccgaa
     1321 gcttcgatga ccaatcgctc gcctcccgcg gcctccgtaa tattctccag ccactcctcc
     1381 agcccagccc cattcacgat caatatgtcg ctgtcagcga tgcgagctac atccttgggt
     1441 gtcggctcga aggcatgtgg atcaagcccc gctggaagca atgtctcaac ggtgaggcgg
     1501 tcgccagcca cgttttggac aatatcagcc aggaaggact cggctgccag tacaatcaag
     1561 cctgcctccc caacctgagg agtcgggact ggattcacct ggcagccata ggcaaggctg
     1621 ataaggaata ctgcaacgat cgtgataatt ttcttcatca aataggctgg gagatttgag
     1681 gtgtgccacg catccctata attatatagc atgggcggca ttcgatggct ggatcaccag
     1741 gccaaaaacc tgttccagtg acccagatat ggaaaagctg aacaagttga cactcggtgg
     1801 ttatttatgg ctggcaatcc tgtgaacaat cacctcttca tagattttat cgagactact
     1861 tcccagcaga aagccgattt tcagcaagtt ggcgcgccgc ggcatccgtc gtaataccct
     1921 gtttcgacgc cacctcaaaa atctgcttca aggcgcgccg gacactctca accacctccc
     1981 gctctgcccg ttcttgcgac caacctttca tttccatgct cgtaatagcc attgcgccgc
     2041 cgacgtttac cacatagtca ggtgcgtaaa gaatacccct agcccgaagg cgctccgcat
     2101 cttcctgggt ggcaaactgg ttgtttgcac ccccaaccac tgcccggcat ttcaactggg
     2161 gaattgtgtc ggcgttgagc acaccaccca gtgcacatgg ggcgaatata tcacattctg
     2221 ttgtgtaaac tgcctcagga ggcacaaact ctaatccgag atcgtcgcgc accttgcgga
     2281 tcgcgccttc gtccacctcg ctatacgcca ctgtagcacc ggcatcgcag aggtaattga
     2341 tcagttttgc cccaacgtca cctgcccctt gtaccagcac tcgccggccg actagggaca
     2401 tgtctccgaa gagatactca caggtctcct ctattccagc gaataccccg cgggctgtga
     2461 aaggcccggg gtccccagct ccgcccgcgg ctggcgtgcg ggcgaatatg tagggagcgc
     2521 cagtctcagc aatgatatcc atatcagctg gcgacgtgcc cacgtctggt ccggtggaga
     2581 acagaccacc gagctggtga atcagcgcac catagcgtcg caatagcgcc ggacgagact
     2641 gggcttcaaa atcggcggga attgcgatga cagcctttcc acctccaaat tccatgccag
     2701 gcgtggcgaa cttgtaggtc atccctgcgg caagtcgcat tgcgtcctgc aaagctgcct
     2761 gcacggaggc atagggcttc atccgcgtgc cacctaccgc aggtccaagg cgggtggaat
     2821 ggatggcgat aaaaatccag gctccagtgg gtcggtcata atgaactatg actgtctcac
     2881 caccccattc cctaatcaaa gtttccatca caagtacgcc tcctttcttg tcgcatcaat
     2941 ttttgagtaa tggcccttga caaaatgaca cctataggtt atataatgag tattatacgt
     3001 tacattatgt gtaatacaag ttaccagaca aatttaaaaa tgatggaaat caaaaaatga
     3061 ccacaaaaac agaaataatg gaccaaaaaa gaaaacggta tcgcagagcc cacgtaattt
     3121 ggttcgcagt gttcttcatc gcatggatta tcagatcagc acttaaactc ttcgaacttg
     3181 acctgcctct gttagagaat atgcttcttt taatactgat attctctgtc gcagtccaag
     3241 ccttttatgc attgaaagac aatatgctgg ccatagagct gaggaaagat cctcagttga
     3301 aagaggcgat gaatgacgaa ttggttcaac taaacgaact gaaagcctgg aaagcagcct
     3361 ttttctcttt aatcggatac attatctttg tagcgctctt atcaatgttc atcgatttca
     3421 atgatttgat gtcgatattc attactgcct tgattattgg ttttggcacg tacaactccg
     3481 ccgccttcgt gttgaacagg tgagattgtg gaagaaaaaa aactaagaaa cacaataaga
     3541 gttcagcgag ccaagaaaaa ccttacccaa gaacaactgg cagaattggt cggtgttacc
     3601 aggaaaacga ttaatacagt agaaaacggt atttatattc cttcaacgta tttagcattg
     3661 aaactatcca gagcattgga cgtaacagtt gaggaattat ttcaactaag tgaatagggc
     3721 taaccgccaa catttgaaat ttttcggtaa tgcttacttg aaaacagtaa aataaaaaac
     3781 aagcccaaat attattatag cggtatttgg gcgcatttcc ctgcttgccc cttatccagg
     3841 tcagctcatg caggctttac ggagcccatc agccagaggc aagaaacgct tcgcaaaatc
     3901 aattgtgcct ctgttcccgt ttgtgacaat agcaacagaa atatttttgc tatattcccc
     3961 gaaagccaaa caagaaaaac ctccagggtg tccaaaacat cggtttgtac tccaccatcc
     4021 aaaacttgac gggaagcgag tccccacaac gaatcccctt cccattgcta ttacagtctt
     4081 aaggcttcta tcccacgaaa gaacatgccg agaggtatat ttctggatcg ccatttcaga
     4141 aatcaactgt ttccctgcgg gtgtcttacc cccagataag agaaaatcat agaaagccgc
     4201 aagactagca gcatcgctga ctaagctggt tgccggattc ctggcattga agaactgctc
     4261 tgaattttgc cattcaaaat cttctgccac gtttacatcg gcgacaagaa aactctgttt
     4321 ccccaaccag taagaaaaag ccaaagtatt gatatctcga ccagccaatc caaactggag
     4381 ggctggcaat tgcaaaggtg cagcgatctc ttccctaaag aagtcaggga gactccttcc
     4441 atctaccctt aaaacaatct cattcagtat ccacccatat tcatggggat ggtatgccaa
     4501 agttccacgc gggtaagaag gaagcgcttc aattatcgct tctcggattg cttcaatatc
     4561 tccgtgaagt ggagtattct tcacaaaatc tggcatcaaa attccggatc tgtgtgtcag
     4621 gacatccagc gtcgtgattt gctctttgcc atgtcgggca aactctggaa agacatgagc
     4681 aattggagcc ttgacatcca atacaccccg atcctccagc atggcgatag caatcgcggc
     4741 aagtggtttg ccagcagata aaacaggaaa cggggtctgg gaatgaacct gcgttggtgg
     4801 aattggctca ctgggtcgga atccacgagc aatccctatt gctatattaa ctactagctt
     4861 tccattacgg ctaaagacaa gctgtccgcc agggaatgaa cccgttagtt gctggttttg
     4921 aaagagatca acaaccctcg ccaacctgcc tgcatccaca ttcaccgatt caggatcaac
     4981 cgtttctttg tacgggaatg ccattcggaa gacttccttt ttttaatttc caagatcggc
     5041 actggttgaa tcccaaccga gcttaccctt ggtcacgatg ataagcatga gcgaaacacc
     5101 tcaatatata caacccctca acaccatgaa tgtacccaat ctgccgagca catccatagg
     5161 tgtcggaata aaacaactga tcacatttgt gccggcatga agcagtatga ccggcat
//
LOCUS       DBNGSX010000518         6445 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_468598, whole genome shotgun sequence.
ACCESSION   DBNGSX010000518 DBNGSX010000000
VERSION     DBNGSX010000518.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6445)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6445)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6445
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_468598"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="AC16CB_15450"
     CDS             <1..190
                     /locus_tag="AC16CB_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR3343918.1"
                     /translation="RKPDKATAKAVIHACQDRNLLLLSCGPWDNTIRWIPPLMVNQHQ
                     IDEALGIFRESLNEVVRE"
     gene            204..1931
                     /locus_tag="AC16CB_15455"
     CDS             204..1931
                     /locus_tag="AC16CB_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HIR3343919.1"
                     /translation="MQLDSPDRFSRVWRVNVRDQSLAINAIPDAWIPLGGRGLLARIL
                     LDEVNPTCAPLGPGNKLIFAPGLLVGHMLSSCDRISVGGKSPLTGGVKESNAGGSTGL
                     QMTRLGIKALIIEDAPQESGWWVLHLSPAGARFDPADDVVGLGVYDSAPKLLERYGQH
                     VAIALIGPGGEMRLSSAGIQNLDKDKVPARIAARGGLGALMGSKGLKAIIIDAKDGEK
                     PPITNLEDFRKAQKEFNKALLAHPQTKIYADYGTAAMPRMSNGFGGLPTRNFSEGTYE
                     NVEKISGEHMRQLLLARGGDSDTTHACMAGCTIRCSNIFGGDDGKAIVSPLEYETIGL
                     LGPNLGISELDDIARMCYQINDLGLDTIEVGAALGVVAEAGLLAFGDSARAMELIDEI
                     GKGTPLGRVLGNGAVTTGVVFGVERVPAVKGQAMSAYEPRAIKGTGVTYATSPQGADH
                     TAGLTIRAKIDHLDPAVQINVSRSAQINMAGYDTLGACVFAGFGFAAAPETIRDLLNA
                     RYDWQVGADILQVLGKETLKLEREFNLGAGFTSAHDRIPEWMTRQPLPPHQAVFDVSE
                     NELDEFFNW"
     gene            1992..2189
                     /locus_tag="AC16CB_15460"
     CDS             1992..2189
                     /locus_tag="AC16CB_15460"
                     /inference="COORDINATES: protein motif:HMM:NF014636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR3343920.1"
                     /translation="MRRLEVSLPDDSTVGDLLSAMEITMPLDSLLLAVNSRVAEVEQV
                     LRDGDQVNIMPAISGGSLNAR"
     gene            complement(2241..3116)
                     /locus_tag="AC16CB_15465"
     CDS             complement(2241..3116)
                     /locus_tag="AC16CB_15465"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3343921.1"
                     /translation="MKSTQSLPENTHIGYTHLMVTDLERSLFFYQDILGFRIIEQEGL
                     TVVLSASGEPPAQMILSTMIGVLPRPPHTTGLYHVAIRLPDRQALSRVFRRALEHQIP
                     FHGAADHLVSEALYLPDPDDNGIELYVDRPRQEWPKLDGQIAMSTDPLDLQDLLNESE
                     NDGKVWDGIAAGTDIGHVHLQVSNLERAEAFYSGILGLDVTQRSYPGALFLSAGGYHH
                     HLGLNIWTSRNAPPPPPEAVGLIAFKLKLPDENARQVLLQRIQDTGQIIEEYSPSSLI
                     SGALIKDPDGIQIIL"
     gene            complement(3200..5731)
                     /locus_tag="AC16CB_15470"
     CDS             complement(3200..5731)
                     /locus_tag="AC16CB_15470"
                     /inference="COORDINATES: protein motif:HMM:NF046780.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 113"
                     /protein_id="HIR3343922.1"
                     /translation="MTKYLITQTREGINRVLPTFILPILLVLVSLACTILPILTPSET
                     PTPGQEIPTPVSATPIPESEVTFRVEIPANSPGDQPVYLNILDEVSGLALNMQTYPMD
                     QEDPLHYSLVLPLPYASAVKYRYSRQGPAAMVEEHVSDGRQMRYRLYHVEGPGVVQDV
                     VTRWTDTKYEGSTGRINGQALDSQNGQPITNLLVVAGGSQALTTSDGSYLLEGLPPGT
                     HNLVAYSLDGTYRTFQQGAVIAPESTTPAELRLAQAPVVNVTFEVNVPAGTIPAVPIR
                     IAGNLVQLGNTFGDLAGGVNTVASRMPVLNLLPDGRYNIQISLPAGADVRYTYTLGDG
                     YWNTELNQDGSRRVRQLIVPESDVIIQDTVDSWLAPDSAPITFDVNVPANTPVDDFVS
                     IQFRPLYGWTEPIPMWHLGNNRWAFVLNNPLQTIGSMSYRYCRDDQCGAADDTQTMGP
                     ASNGYPINSSLFQQTMKDQVDAWAWLDSNPITVAVDPSDVTTVGSHFMAGIEMQPGYH
                     PTWKPRAPEALTELQGLGANWVFLTPTWTYTRNTPPILELASGRDSPWYDIQDNIQQA
                     RQRNLSVAIYPIAQLPSPTNQWWAEAKRDFPWWSVWFERYRIFALHHADLAARNDAQA
                     LILGGDWLLPAMPNGTLTDGTPSGVPADAETRWRNLIQEVRTHFGGVIIWAIPYSQAA
                     NNPPVFLDSVDRIYVHFNPQLSTVTNSGRPDLQAEAAKLLDTGVLPLQARFGKAIMIG
                     VEYPSVDGAALGCLNGLPDGCLNASALARPNPDVSGFPIDLGEQADIYSGLLSAINGR
                     SWITGFISRGYYLPVMLQDKSASVRGKPAQEVLKYWFPRLLGTIQ"
     gene            complement(5728..>6445)
                     /locus_tag="AC16CB_15475"
     CDS             complement(5728..>6445)
                     /locus_tag="AC16CB_15475"
                     /inference="COORDINATES: protein motif:HMM:NF042691.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6585 family protein"
                     /protein_id="HIR3343923.1"
                     /translation="WSEAVTLILPGILGVLTPLLYGVARARYGILNYGPVAADQWSRP
                     WYLLAIIAYLIFAFVGISRIMASRRYFAIYEGGVAHNLGRKMTFAWEEIAGITTETTQ
                     NQFLGILLRPYVQGYIYPNIGEPVRLDNAIQNLPELLTILKAKLYPRLLPRLQSNLES
                     GQWLYFGSLAIQQKGIKLLNKHQSNRAQAIPWSQITFVNIESGYMVVELSDRPRLKLP
                     VSKIPNIELLLQLIKMGVNS"
BASE COUNT         1560 a         1523 c         1763 g         1599 t
ORIGIN      
        1 tcgaaagcca gataaggcga cagcgaaagc ggttatccat gcttgccagg accgtaactt
       61 gcttttgctt tcatgcggtc cgtgggataa cacaatacga tggatacccc cgttgatggt
      121 gaatcaacac cagattgatg aagcgctggg catcttccgt gaatcactga atgaggtagt
      181 aagagaatag caggaggtat gctttgcaat tagatagtcc ggatcggttt tctagagtgt
      241 ggcgcgttaa tgtacgggat caaagcctgg ctattaatgc catccctgat gcttggatcc
      301 ctttaggtgg acggggcttg ttggcgcgca tcctattgga tgaagtcaac cctacctgtg
      361 cacctctggg acctgggaat aagctgattt ttgcaccagg tttgcttgtt gggcatatgc
      421 tttccagctg cgaccgcatt tcagtgggtg gcaagagtcc cctgaccggc ggtgtaaagg
      481 aatctaatgc aggcggttcg acgggcttgc aaatgactcg cctagggatc aaggcattaa
      541 ttatcgagga tgcaccacag gagtcaggat ggtgggtttt gcatctatct ccagctggag
      601 cccgttttga ccctgcggac gacgtggttg gattgggcgt gtacgattct gcccccaaat
      661 tgcttgagcg ttatggtcag cacgtagcta ttgccctcat tgggccaggt ggtgagatgc
      721 gcctctcgag tgcgggtatc cagaacctgg ataaagacaa ggtgcctgcg cgcatcgcgg
      781 ctcgtggcgg tctaggagct ttgatggggt caaaaggtct caaagcgatc atcatcgatg
      841 cgaaagatgg tgaaaaacct cccataacca accttgaaga ttttcgcaag gctcagaaag
      901 agttcaacaa agcgctctta gcccacccgc aaactaagat ctacgccgat tatggaactg
      961 cagccatgcc gcgcatgtcc aatggatttg gggggttgcc aacccgcaat ttctcggagg
     1021 gtacttacga gaacgtcgag aaaattagcg gcgagcacat gcgccagtta ttgttggctc
     1081 gcgggggcga cagcgataca acgcacgctt gcatggctgg ctgtacaatt cgttgctcga
     1141 acatcttcgg tggtgatgac gggaaagcca tcgtgtcgcc acttgagtat gagacgattg
     1201 gcttgctagg cccgaacctg ggtatatctg agctggatga cattgccaga atgtgctatc
     1261 aaataaacga tcttggtttg gataccatcg aagtgggggc tgctctgggc gtcgttgctg
     1321 aagctggatt gctggcattt ggtgacagtg ctcgggcgat ggagctgatc gatgagattg
     1381 gcaagggaac acctttaggg cgcgtgctgg gcaacggcgc agtcacgacg ggagtagttt
     1441 tcggtgttga gcgtgtgccg gcagtcaaag ggcaggcgat gtctgcctac gagcctcggg
     1501 ctatcaaagg tacgggagtt acttatgcca cctcgccgca aggggctgat cacaccgctg
     1561 gcttgaccat tcgcgccaaa atcgaccact tggacccggc tgtgcaaatt aatgtgtcac
     1621 gtagtgccca gatcaatatg gctggttacg acaccctggg ggcttgcgtt tttgctggat
     1681 ttggctttgc tgcagctccg gaaacaatac gagatttgct taacgctcga tatgattggc
     1741 aggtcggtgc agatatcctg caagtgttag gtaaggaaac cctcaagcta gagcgggaat
     1801 tcaacctggg ggctggtttt acttcagcac acgaccgaat ccctgagtgg atgactcgcc
     1861 aaccgctacc accacaccaa gccgtattcg atgtgtctga gaatgaattg gatgagtttt
     1921 tcaactggta agttttgaga gttactgttc accttcacac aatattacaa aggcagacac
     1981 ccgagggtct tgtgcgccgt ctggaagtat cacttccaga tgacagcacc gtgggtgact
     2041 tgctaagcgc aatggagatt actatgcctc tcgattcgct cttgctagcg gtcaatagtc
     2101 gtgtagctga ggtggaacaa gtattgaggg atggcgatca agtgaatatt atgccagcaa
     2161 tttcaggggg ctcgttaaat gctcggtaaa agaatgaggt tcattcctgc gcaatctgat
     2221 ttatattaat ctcaatcgaa ctataaaatg atttggatcc cgtccgggtc ctttattagt
     2281 gcacctgaaa tcagactgct tggagaatac tcctcgataa tttgccctgt atcctggatt
     2341 cgctgtagta aaacttggcg cgcattctca tcaggcagtt tcaacttgaa ggcgatcaac
     2401 cccactgctt caggcggtgg gggaggagca ttacggctgg tccatatatt caaccctaaa
     2461 tgatgatggt atccaccagc agatagaaaa agggctcccg gatagcttcg ttgggtcaca
     2521 tccaagccga gaatccctga ataaaatgcc tctgcccgtt ccaaattaga tacttgcagg
     2581 tgaacgtgcc cgatatcggt tccggcggcg atgccatccc acactttacc gtcattttct
     2641 gattcattga gcagatcttg caaatccaag ggatctgtag acatagcaat ctgaccatct
     2701 aacttgggcc attcttggcg agggcggtct acgtatagct caatgccgtt atcatcagga
     2761 tcgggtaggt agagcgcctc gctaactaag tggtcagcgg caccatgaaa aggtatttgg
     2821 tgctctagtg ctcgtcggaa cacccggctt aatgcctggc gatcaggcag gcggatagca
     2881 acgtgataca acccggtggt atggggaggc cttgggagaa caccgatcat ggtggacagt
     2941 atcatttgcg caggtggttc tccgcttgca gacaatacca cggttaagcc ttcctgctcg
     3001 atgatacgaa atccgaggat atcttggtaa aagaagagtg accgctctag gtcggtgacc
     3061 attaggtggg tatagccaat gtgagtgttt tcaggtaaag attgcgtact tttcattgct
     3121 ttttaattag agcagttggg taggcgatgc ccacccaact agtccatcga ttgtgtatac
     3181 tctataaacc aaaaggaatt tattgtattg tcccaagaag gcgtgggaac cagtatttta
     3241 agacctcttg agcgggtttt ccacgcactg aagcggattt gtcttgaagc atcacaggta
     3301 gatagtatcc tcggctgata aatccggtga tccagctgcg accattgatg gcgctcaata
     3361 acccgctata gatgtctgct tgttcgccta aatcgatggg aaatcctgat acatcaggat
     3421 ttggtcgggc aagagcgcta gcgtttaagc aaccatcagg aagaccgttc agacagccca
     3481 aagccgcgcc atcaacggac ggatattcta ctccaatcat aattgccttg ccaaagcgcg
     3541 cctgtagagg caaaacccca gtgtccagca gcttggcagc ctcggcctgt aaatccggtc
     3601 tccctgaatt ggtgacggtg gataattgtg ggttgaaatg tacgtagata cgatctacag
     3661 aatccaagaa aacaggtggg ttgtttgcag cttgtgaata aggtatcgcc catatgatga
     3721 cgccaccgaa gtgtgtacgg acttcctgaa tgagattacg ccagcgagtt tctgcatctg
     3781 ccggcactcc tgatggtgtt ccgtctgtta atgtgccgtt aggcatagcg ggcagtagcc
     3841 agtctccgcc taaaatcagg gcttgtgcat cgttgcgcgc tgccaggtcg gcgtgatgca
     3901 gagcaaagat gcggtaacgc tcaaaccata cagaccacca cggaaaatct cgtttggcct
     3961 ctgcccacca ttgattcgtt ggtgagggca actgtgcgat ggggtaaatt gcaacgctca
     4021 ggttgcgctg gcgcgcttgc tgaatgttat cctgaatatc ataccaggga gagtcgcgcc
     4081 cacttgctaa ttccagaata ggtggcgtat tgcgtgtata agtccaggtt ggggtcagga
     4141 aaacccaatt ggcgccgagc ccttgtaatt cagtcagcgc ttcaggagcg cggggtttcc
     4201 acgtaggatg atagccaggc tgcatctcta ttccagccat aaaatgagag ccgaccgttg
     4261 tcacatcaga tgggtcaacc gcaacggtga tcgggtttga atccagccac gcccaggcat
     4321 ccacttgatc cttcatggtt tgttgaaaca agctggaatt gatcggatac ccgttacttg
     4381 ccgggcccat tgtttgggta tcatcagctg ccccacactg gtcgtcccgg cagtagcggt
     4441 aactcatact gcctatcgtt tgcaacgggt tgtttagaac gaaagcccaa cgattgttcc
     4501 caaggtgcca cattggaata ggttctgtcc agccataaag aggcctgaac tgtatcgaaa
     4561 caaagtcatc cactggagtg tttgctggga cgttcacatc gaaggtaatt ggtgcagaat
     4621 cgggggctaa ccaagaatcc actgtatcct ggataatgac atcactttct ggcacaatga
     4681 gctgccggac acgtcggctg ccatcctggt taagttcagt attccaatag ccatctccaa
     4741 gcgtgtaggt gtaacgcaca tctgcgcctg cgggtagaga tatttgtatg ttgtatcgcc
     4801 catcgggcag aagatttagc actggcatac gtgatgccac cgtgttaact cctcccgcta
     4861 gatcaccgaa tgtatttccc aattggacca ggttgccagc gatgcggatg ggtacagctg
     4921 ggatggtgcc ggcggggacg tttacctcaa aggtgacatt cacaaccggg gcctgagcta
     4981 agcgcagttc cgcaggtgtc gtagattcag gcgcgataac cgccccttgt tggaaagtgc
     5041 gataagtccc gtcgagagag taggcaacca ggttgtgcgt acccggcggc aatccctcaa
     5101 gcaagtatga gccatccgaa gtggtaagcg cttgtgagcc gccagcgaca accagcaggt
     5161 tggtaattgg ttgaccgttt tgtgaatcaa gggcctgccc attgatgcgc cccgttgagc
     5221 cttcgtattt tgtgtctgtc cagcgggtta ccacatcctg cactacgcct gggccctcaa
     5281 cgtgatacag cctgtaacgc atctggcgac catctgagac atgctcctct accatggctg
     5341 cgggtccttg acgcgaataa cggtacttca ctgcggaggc atagggtaac ggcaaaacca
     5401 aggagtaatg aagtggatct tcttgatcca tcggatatgt ctgcatgttt agagccagcc
     5461 cgctcacttc atctaaaata ttcaagtaaa ccggttggtc tccgggcgag tttgccggga
     5521 tctctacgcg gaaagtaacc tcgctctccg ggatgggggt tgcgctaacg ggagtaggaa
     5581 tctcttgccc aggtgtgggc gtctctgagg gcgtcaggat cggcaggatt gtgcaagcca
     5641 ggcttaccag cacaagcaag atgggtaaga tgaaagtcgg taagactcga tttatacctt
     5701 cgcgggtttg ggtaatgaga tattttgtca cgagttcacc cccattttga ttaattggag
     5761 gagtaattcg atatttggga tttttgagac cggcaacttc agacggggtc ggtcggataa
     5821 ttctaccacc atatatccag attcaatatt gacaaatgta atctgagacc agggtatggc
     5881 ttgggcacga tttgattgat gcttatttag caatttgatc cctttttgtt ggatagcgag
     5941 cgagccaaag tataaccatt gcccgctttc tagatttgat tgaagacgtg gcagcaagcg
     6001 cggatagagc ttcgccttaa ggatagtaag tagctctgga agattttgta tagcgttgtc
     6061 caggcggaca ggctctccaa tgtttggata aatatatcct tgaacataag ggcgcaatag
     6121 gatccccaag aactggtttt gggtggtctc agtggtaatt ccggcaatct cctcccaagc
     6181 gaaggtcatt tttctcccta aattatgagc cactccgcct tcataaatgg cgaaatagcg
     6241 gcgggatgcc attatgcgag agatgccaac aaatgcaaaa atgagataag caatgatcgc
     6301 aagaagatac cagggtctac tccattgatc ggctgccacc gggccgtagt ttaatatccc
     6361 gtagcgagcg cgtgcaacgc catataacaa aggtgtgagc actcccagga tgcccggcaa
     6421 gatcagtgtc acagcctcac tccag
//
LOCUS       DBNGSX010000519         2975 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_815032, whole genome shotgun sequence.
ACCESSION   DBNGSX010000519 DBNGSX010000000
VERSION     DBNGSX010000519.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_815032"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="AC16CB_15480"
     CDS             <1..347
                     /locus_tag="AC16CB_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIR3343924.1"
                     /translation="MDEMLGKLRERIRSHTQLATTIGYGPRFLHSTGQLHKGDAGNGL
                     FIQFTSQLQGDVAIPDEADLPDSSMSFGVLKLAQALGDRQALLDNRRRIIRIHLGERP
                     VASLETILKQLI"
     gene            426..1730
                     /locus_tag="AC16CB_15485"
     CDS             426..1730
                     /locus_tag="AC16CB_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343925.1"
                     /translation="MEKIFPSQHTSAWQWIALVLWTLFALAFLAFFLIDSRLDYIQLL
                     APCKGTDCNWMAISSAEVELLESWGLYTRAYAALMIGTAALTVAVYWLLGGLVLLRQG
                     AARFGLAVSLVLLSIPIAMISDPDNLYASYPALLLPSIFLQSLGTIFLLLFLYLFPNG
                     RFYPRLASIPLAGAVLIILISDVLEVSGFDLDSPVQITLFQALFMMLALGTVFQIFRY
                     RRDSTLLERQQTKWVLFGFSILTLSFPLWGSIFGGFLEIPPGEARLLANILGWIIIQF
                     ILIALPISIAIAILRYRLWDIDFIIRRTLIYGGLTLTLAAVYFGSVVLLQALSQRVVG
                     QESTVAIVLSTILIAALFNPLHKRIQSDIDQRFYRKKYDTQRTLETFAASLREEVELE
                     QLTGQLQAVVQETMQPEHVSLWLRTPDANEAINLQPPKASVD"
     gene            1768..2526
                     /gene="ribA"
                     /locus_tag="AC16CB_15490"
     CDS             1768..2526
                     /gene="ribA"
                     /locus_tag="AC16CB_15490"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HIR3343926.1"
                     /translation="MSLTHMQIKDLLDENRDHSCEGFGLDHICVKIAAIADLPSRFGK
                     FHAVAFYNNRDDKEHAAFVHGDICNAENVPVRVHSECLTGDAIGSLRCDCRDQLEIAL
                     TTIGQMENGILLYMRQEGRGIGLTNKLRAYGLQDQGYDTVEANLALGFKDDERDYSVA
                     AHMLHSLKVKSIQLMTNNPRKINELAAYGINIAGRIPLIIPPNPYNQFYLETKAEKSG
                     HLLDPNGKEHLFEQMDRPVLPGMTHEQIEILQEN"
     gene            2529..>2975
                     /locus_tag="AC16CB_15495"
     CDS             2529..>2975
                     /locus_tag="AC16CB_15495"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3343927.1"
                     /translation="MDFHDRVAVITGATGGLGRVVARKFGETGARLALLSSNQDTLNR
                     LVDELSLPADRYMTKALDFRNPDAAKIAAQATVDKYGRADILVHLIGGWTGGKPLVEV
                     EPNQLEEMIQQHVWTTFYLVQSFVPVFHANHWGRMVVISSPNAGMPS"
BASE COUNT          713 a          783 c          727 g          752 t
ORIGIN      
        1 agatggatga aatgcttggc aagctgcgcg agcgaattcg ctctcatacc caattagcga
       61 ccacaattgg atatggtccg cgcttcttgc actccacagg ccagctacac aaaggcgatg
      121 ctggaaatgg cttgttcatc cagtttacct ctcagttaca gggagatgtc gctatccccg
      181 acgaggctga tctcccagac tcgtcgatga gttttggagt attaaagttg gcacaggccc
      241 tcggtgaccg gcaggcattg ttggacaata gacgccgtat cattcgcatc catttgggcg
      301 aaaggccagt cgccagtctg gaaaccatct tgaaacaact gatatgatcc atccagtctc
      361 cccttagtag ttgagagcat gaacaaggag cacaataacc gggttcacca gaatagtgag
      421 gattaatgga aaaaatattt ccgtcacaac atacctctgc ttggcaatgg atcgcgctgg
      481 tattatggac attatttgcg ctggcattcc tcgccttttt cctgatcgat tcgcgcttgg
      541 attacatcca gcttctggcg ccttgcaagg gcacagattg caactggatg gcaatttcat
      601 cagccgaggt tgagcttctc gagtcctggg ggctatacac ccgggcttat gctgctttga
      661 tgatagggac cgccgctttg accgtggctg tttactggct attgggcggg ttggtcctgt
      721 tgcgacaggg agccgcccga tttgggctag cggtttccct tgttttactg tccatcccca
      781 ttgcgatgat ttcagaccct gataatctat acgccagcta tccggctctg cttttgcctt
      841 cgatttttct ccaatccctt ggcacgatct tcctgctgct gttcctttac ctcttcccta
      901 acggacgttt ttacccccgc ttggcatcca tccccctggc aggagccgtc ttaatcattt
      961 tgatcagcga tgtcttagaa gtcagtggat tcgaccttga ttcaccggta caaattacat
     1021 tattccaagc cttattcatg atgctagccc tgggtactgt ctttcaaata tttcgctaca
     1081 gaagagattc cacgctccta gaacgccagc aaaccaagtg ggtcttgttc gggttttcta
     1141 tcctcactct cagttttcca ctctggggat ccattttcgg tggattccta gaaatacccc
     1201 ctggagaggc caggttgctg gcaaacatac ttggctggat cataatccaa ttcatcttga
     1261 tagccctgcc aatttcgatc gccatcgcca tcctgcgcta ccggttgtgg gatattgact
     1321 ttatcatccg ccgcacatta atttatggtg gtctcacgct caccttggcc gcggtctact
     1381 tcggtagcgt tgtcttgcta caggctctct cccaacgcgt tgtcggccaa gagtcaactg
     1441 tggcgatcgt cctttctact atcttgatcg ccgccctgtt caacccgcta cacaagcgca
     1501 tccagagcga catcgaccag cgcttctatc gaaagaaata cgacacacaa cgcacgctgg
     1561 agactttcgc tgccagcctg cgcgaggagg tcgagctgga acaattgacc ggacaactcc
     1621 aggcagtcgt gcaggaaacc atgcagccgg agcacgtcag cctctggttg cgaacgcctg
     1681 atgcaaatga agcgattaac ctccaaccac ctaaagccag cgtggattaa ccagaagatt
     1741 gaaattcttc gtatacaagg agatcgcatg tcactcactc atatgcaaat aaaagactta
     1801 ttggatgaaa accgcgacca ctcttgtgaa ggttttggcc tggatcacat ttgtgtcaag
     1861 atagctgcca tcgctgacct gccttcgcga ttcggcaaat ttcacgcagt ggcattttac
     1921 aacaatcggg atgataaaga acacgccgca ttcgtgcacg gggatatctg taatgcggag
     1981 aatgtacctg tcagggtgca ttcagaatgc ctgacagggg atgccatcgg ctccttgcgt
     2041 tgcgactgcc gtgatcagct tgagatagct ttgaccacaa tcggtcaaat ggaaaacggc
     2101 atactgctgt atatgcgcca ggaagggcgc gggatcggat tgaccaataa gttgcgtgcc
     2161 tacggtctgc aagatcaagg ttatgacacc gttgaagcca atctagcgct cggcttcaag
     2221 gatgatgagc gtgattatag tgtggcggca cacatgctcc actcgctaaa agtcaaatca
     2281 atccagttga tgaccaataa cccgcgcaag attaatgagc tggctgcgta tggcatcaac
     2341 atcgcgggac gcatcccatt gatcataccg cccaacccct ataaccaatt ttatctagag
     2401 accaaggccg aaaaatctgg ccatttgctg gacccaaatg gcaaagaaca cttatttgag
     2461 caaatggatc gaccagtact acctggcatg acgcacgaac agattgaaat tctgcaggag
     2521 aattaaacat ggattttcat gaccgcgtcg ctgtgatcac tggagcaaca ggaggcctgg
     2581 ggcgggttgt agcccgcaag tttggagaga caggtgcgcg gctggcacta ttgagctcaa
     2641 accaagacac tctcaatcgg cttgttgatg agctgagtct ccctgcggat cgttatatga
     2701 cgaaagcact ggactttcgc aatccagatg cggcaaaaat cgcagcgcag gcgacggtgg
     2761 ataaatatgg tcgcgcagat atcctcgtgc atctcatcgg tggctggact gggggcaagc
     2821 cccttgtcga agtagaaccc aatcaattgg aggaaatgat ccagcagcat gtctggacca
     2881 ctttctacct ggtacagtca ttcgtgcccg tctttcatgc caatcactgg gggcgcatgg
     2941 tagtcatttc ttcccccaat gctggcatgc catct
//
LOCUS       DBNGSX010000520         2762 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1853392, whole genome shotgun sequence.
ACCESSION   DBNGSX010000520 DBNGSX010000000
VERSION     DBNGSX010000520.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1853392"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="AC16CB_15500"
     CDS             <1..246
                     /locus_tag="AC16CB_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
                     /protein_id="HIR3343928.1"
                     /translation="AGEAAGGIHGRNRLMGNSLLDICVFGRRAGVSAALWAQGVQPGH
                     PTLAHLRDWERARRDSGLDEAAASPMILPDYTVREHL"
     gene            359..598
                     /locus_tag="AC16CB_15505"
     CDS             359..598
                     /locus_tag="AC16CB_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343929.1"
                     /translation="MSDHASDLDKCTEDGPQSALERKLIEEYLSEKGYHLSDLQALPE
                     REARRLMREACKYASLKLAEVESRAQLRKEIHYPS"
     gene            609..1970
                     /locus_tag="AC16CB_15510"
     CDS             609..1970
                     /locus_tag="AC16CB_15510"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015590050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0004473 - malate dehydrogenase
                     (decarboxylating) (NADP+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent malic enzyme"
                     /protein_id="HIR3343930.1"
                     /translation="MLKEKPSVNVDELLAKAKKPSADALKLHPFYRGKVETTLKCVVR
                     DFRDFAIWYTPGVAAPCREIAANPEKVYEHTNKWNTVAVVSDGTRVLGLGDIGPKAGL
                     PVMEGKALLYKYLGGVDGVPIMLDTKDPDAIINTVLLLQPGLGGVNLEDISQPKCFYI
                     LDTLREKAEIPIWHDDQQGTATVTLAGLMNALKVVGKKKEEARIAFIGCGAANVACSR
                     LIYGWGVDPANCIMVDSKGILGMHRKDLELRKAEFVDKWRLCQITNAEGRQGDIPAAM
                     KGMDVVIALSRSGPGVILPEWVSSMEKDAILFACANPIPEIWPWEAREAGARVVATGR
                     SDFPNQVNNSLGFPGIFRGALDVRAKTITDEMCYAASQALADQIGDRLDEEHILPTMD
                     DWEVYPREAAAVAMKAQEQGLSRLQKSYDELLENAYAIIRRSRDLTQMMMKQGFIPEA
                     PAE"
     gene            1993..>2762
                     /locus_tag="AC16CB_15515"
     CDS             1993..>2762
                     /locus_tag="AC16CB_15515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3343931.1"
                     /translation="MYDLIVIGGGPAGLTATIYAIRKRLNVLLVSRDLGGKTNYRLSL
                     PWIEDYQVVRGLEVVNKFKSELEYLKFARLLQAVERVERRPEGFAVFTEEGRELLTRA
                     VIIATGTRQKFLSVPGERDFMMRGLCYSATSYAPLFIDRKTVVVGDGELALRSTAELA
                     TVAKHVHLVGVNPDALELPLGQKLKAAENVTILQSYQVVEVKGNGYAERIMVKAPDGH
                     ADQIEADGTFIEIGLIPNSKMVEDLVNLDQQGRIVVDCL"
BASE COUNT          626 a          685 c          858 g          593 t
ORIGIN      
        1 gccggggagg cagccggagg tattcatggg cgtaatcgcc tgatgggcaa tagtctgctg
       61 gacatctgtg tctttgggcg ccgggcgggt gtcagcgctg ccttgtgggc gcagggtgtc
      121 cagccggggc atcccacgct cgcccacctg cgggattggg agcgcgctcg ccgtgatagc
      181 ggactggatg aggctgctgc ttcgccgatg atcttgccgg actatactgt gagggagcat
      241 ttgtaaacca agtaatttct tcgtcgtatc tttgccgcct ggggtgaaaa ccggtatctg
      301 taggaagatt actgtttttg taaattagat tagtaaacca ggcatatgga ggtgaaccat
      361 gtctgatcat gcttcggatc tcgataagtg taccgaagat ggacctcaat cggcgttgga
      421 aaggaaactc atcgaggaat atctgtccga gaaaggttat catctgagcg atctccaagc
      481 tctaccggag agggaggcga ggcgtttaat gcgagaggcg tgtaagtacg cctccttgaa
      541 gctcgcagag gtcgaatcgc gcgctcagct acgcaaggaa attcattacc cttcgtaagg
      601 agtgtctcat gttgaaagaa aaaccatctg tcaacgtcga cgagctgctt gccaaagcca
      661 agaagccgtc ggcggacgca ctcaaactgc acccgttcta ccgggggaaa gttgagacga
      721 cgctcaagtg cgtcgtgcgc gattttagag atttcgcgat ctggtatact ccgggggtag
      781 ctgcgccttg ccgggagatc gccgcaaacc cagagaaagt gtatgagcat accaacaaat
      841 ggaacacggt ggcagtcgtc agcgatggca cacgcgtgct gggattgggt gatatcggcc
      901 caaaggccgg tctgcctgtg atggaaggca aagccctgct gtataaatac ctgggcggcg
      961 tggacggcgt tccgatcatg ctggatacca aagatcccga cgccatcatt aacacggtgt
     1021 tgttattgca gcccggtttg ggtggcgtga atctggaaga tatctctcag cctaagtgtt
     1081 tctatatcct ggatacgctg cgggagaaag ctgaaatccc catctggcac gatgaccagc
     1141 agggcactgc cacggttacg ctggcaggcc tgatgaatgc tctcaaggtc gtagggaaga
     1201 aaaaagaaga ggccaggatt gccttcattg ggtgcggtgc agcaaatgta gcctgctctc
     1261 gcctcatcta tggctggggt gtcgatcctg ccaattgtat tatggtggat agcaagggca
     1321 tcctgggcat gcaccgcaaa gacctggagc tgcgcaaagc tgagttcgtc gataagtggc
     1381 gactttgcca gatcaccaat gcagaaggtc gccagggcga tatacccgcg gcgatgaagg
     1441 gtatggatgt cgtcatcgcc ctctcccgct ctggaccagg tgtgatctta ccagagtggg
     1501 tgtccagcat ggaaaaggac gcgatcctgt ttgcctgcgc caacccaata cctgagattt
     1561 ggccctggga ggcgcgagag gctggggcgc gggttgtcgc aacagggcgt tctgatttcc
     1621 cgaaccaggt aaacaactcg ctaggtttcc cgggcatctt tcgtggtgct ttggacgtgc
     1681 gcgccaagac catcacggac gagatgtgct acgctgcctc acaggcgctg gctgaccaga
     1741 tcggcgaccg gctggacgag gaacacatct tgcccaccat ggatgactgg gaagtgtatc
     1801 cacgggaagc agcggctgtg gcgatgaagg ctcaggaaca aggtctgtcg cgactccaga
     1861 agagttacga tgagctgctg gaaaatgcgt atgcgatcat ccggcgttcg cgcgacctca
     1921 cccagatgat gatgaagcag ggcttcatac ccgaagctcc cgctgagtga tggaaaaagg
     1981 acgaggtgcc ccatgtacga tttgattgtg atcggtggag gccctgcggg cctcaccgca
     2041 acgatatacg ccatccgcaa gcgcctgaat gtgctgctgg tttccagaga tttaggcggt
     2101 aagacaaatt accgcttatc gctgccctgg attgaggact accaggtggt gcgcgggttg
     2161 gaggtggtca acaaattcaa gagcgaattg gaatatctca agttcgcccg cctgctccaa
     2221 gcggttgagc gcgtagagcg ccgtccggag ggctttgcag tgtttaccga ggaaggcagg
     2281 gagctgctca cccgagcggt catcattgcc acagggacac ggcaaaagtt tctttctgta
     2341 ccgggtgagc gagatttcat gatgcgtggc ctgtgctatt cggcaaccag ctatgcaccg
     2401 cttttcattg accgcaaaac tgtggtggtt ggtgacggcg agctggcgtt gcgctcaacg
     2461 gctgagctag ccacagttgc taagcacgtc cacctggtgg gggtcaatcc cgatgccctg
     2521 gaactccccc tgggacagaa gctcaaggcg gcggaaaacg tcaccatcct gcagagctat
     2581 caggtcgttg aagtgaaggg gaatggttat gccgagcgca tcatggtaaa ggcgccagac
     2641 ggccatgcgg atcaaattga agccgatggg acgtttattg agatcggttt gatacccaac
     2701 tcgaagatgg tcgaagacct ggtaaatctc gaccaacagg ggcgaatcgt ggtggattgc
     2761 ct
//
LOCUS       DBNGSX010000521         8328 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_745895, whole genome shotgun sequence.
ACCESSION   DBNGSX010000521 DBNGSX010000000
VERSION     DBNGSX010000521.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8328)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8328)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8328
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_745895"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="AC16CB_15520"
     CDS             complement(<1..262)
                     /locus_tag="AC16CB_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343932.1"
                     /translation="MPRHDLNRDLSPLQDLRPQQALALQARSGQERWGYKAFNGFYCD
                     IRSGPDQVDAMDKALPNGKEMRERGIVADYLRHPSAPLNVILE"
     gene            complement(328..1824)
                     /locus_tag="AC16CB_15525"
     CDS             complement(328..1824)
                     /locus_tag="AC16CB_15525"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HIR3343933.1"
                     /translation="MSYPNFLAVDLGAESGRVILGGLQENRLELQEVHRFDNRPVRVG
                     SHLYWDVLRLWSDIQHGLALAHHQAGESLVSLGVDTWGVDFGLLDANDELLGNPYHYR
                     DRRTDGMLEQAFSVISREEIYHQTGIQFMQINTLYQLLAMAKADSPLLAAGRTFLNMP
                     DLFNFWLSGEKTSEFTIASTTQCYTPLERDWARSMLNRLGIPDQIFGQIVPPGTKLGK
                     LRPTLAADINCGPVMVIATAGHDTACAVVAVPASSPDYIYLSSGTWSLMGIESRQPVI
                     NEDSLAFNLTNEGGVNDTFRVLKNIMGMWLLQECRREWALQGQSYSYAELTQMAAQAQ
                     ALQSFVRPSDPRFLHPSDAQAGTMVSRIQSFCQETSQPVPRSNGEITRCILESLALEY
                     RRTVEQLSAISDMELPVIHIIGGGSRNQLLNQLTADATGRVVVAGPVEATAIGNILVQ
                     AIATGHLTSLEEARSLVRKSGQITTWEPSNDSGWDDAYARYQAWTVSI"
     gene            complement(1837..3639)
                     /locus_tag="AC16CB_15530"
     CDS             complement(1837..3639)
                     /locus_tag="AC16CB_15530"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008103463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HIR3343934.1"
                     /translation="MKTIQINPPHNRLIGSMPKIGIRPTIDGRHQGVRESLEEQTMNM
                     ARNVAAFLSENLRHANGLPVECVIADTCIGGVAEAAQTAEKFARAGVGVSLTVTPCWC
                     YGSETMDMDPIIPKAVWGFNGTERPGAVYLAAVLAGHSQKGLPAFGIYGRDVQDSGDN
                     SIPEDVQEKLLRFARAGLAVATMRGKSYVSMGGVSMGIAGSIVDQDFFESYLGMRVET
                     IDMVEFIRRINQNIYDPVEFDEALKWVRANCKEGQDTNPLDQQRSREQKDKDWEVSVK
                     MAMIARDLMVGNPRLAEIGFGEEALGHNGIAAGFQGQRHWTDFMPNGDFLEAILNSTF
                     DWNGIRQPYIVATENDSLNGTCMLFGHLLTGSAQIFSDVRTYWSADAIERVTGYRPTG
                     LAENGVLHLINSGPSALDGTGQQEVDGKPAIKPWWDVTAEEAAKCLDVTEWCASITEY
                     FRGGGWSTDFLTRGGMPVTLSRLNLVKGLGPVLQLAEGFVVDLPTEVHDALDSRTNST
                     WPTTWFVPRTDGSGVFRDPYSVMNAWGANHGAFSYGHIGADLITLASMLRIPVYMHNV
                     SKERIFRPSAWFAFGTQDLEGADFRACANFGPLY"
     gene            complement(3793..4182)
                     /gene="rbsD"
                     /locus_tag="AC16CB_15535"
     CDS             complement(3793..4182)
                     /gene="rbsD"
                     /locus_tag="AC16CB_15535"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HIR3343935.1"
                     /translation="MIKTGILNPQILSLISRVRHTNTLVIADRGFPYWPMIETVDISL
                     VDGIPTVLQVFNALKSNFIIGQVYMAEEFLKENSPEIQQAFANSRGDIPVIYESHADF
                     KLRVPQAIGLIRTGETIQYANMILVSS"
     gene            complement(4279..5259)
                     /locus_tag="AC16CB_15540"
     CDS             complement(4279..5259)
                     /locus_tag="AC16CB_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR3343936.1"
                     /translation="MNTKKVLFALLSALVLISVIAGCAPAAQPAAPAEPAAKPYIPVI
                     SKGFQHQFWQAVKLGAEQAATDLNVDITFEGPESEAMVDKQVEMFQTALDKKPAAICL
                     AAVDSKAFQNLLEKAKDAGIPVVGFDSGVDSDIPVTTAATDNIAAAALAADKMVELIG
                     GEGEVAVIAHDQTSRTGIDRVAGFVDQVKNKYPNVTVVDVQYGGGDQLKSTDLAKAII
                     QAHPDLKGFFGANEGSIIGVLNAVAELGMEGQITVIGYDSGQQQMDAIRAGTEAGAIT
                     QNPVGIGYKCVEAALKAYNGEELPKTIDTGFYWYDKSNIDEPQIQAVLYK"
     gene            complement(5390..6376)
                     /locus_tag="AC16CB_15545"
     CDS             complement(5390..6376)
                     /locus_tag="AC16CB_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015846877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3343937.1"
                     /translation="MNSEPKTAMTLFRSDAMQRIMAFGALIVLAGGFSLASPFFRTFD
                     NFVGILLATAVNGVLGLGATFVIITAGIDLSVGTVLTFSAVMTGVLITNLHLPIPLGI
                     LCGILAGGLAGLVNGLMISRMKIPPFIATLGMLYATKGLSLVISELKPIYFNDTPAFR
                     EMAMGSALGNIIPGFEVPNAVFIMFGAAIIANLILSRTILGRYTFALGSNEEATRLSG
                     VNVAFWKTAVYTLCGLFSGLGGVLMASRLNSAQPALGAGYELDAIAAVVIGGTSLSGG
                     EGSILGTIIGAFIISTLTNGLRILSVPQEWQMVVTGGIVILAVFLDILRRRR"
     gene            complement(6414..7934)
                     /locus_tag="AC16CB_15550"
     CDS             complement(6414..7934)
                     /locus_tag="AC16CB_15550"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR3343938.1"
                     /translation="MSKVLILMEGIEKRFPGVHALNQCRFELRSGEVHALVGENGAGK
                     STLMKILGGIYHKDAGSIYLEGQEVEIQNPRMAQELGISIIHQELNLMPHLTVAQNIF
                     IGREPRSNIPFIVDDRITNQHAERLFKMLNLQLDPVAKVADLTVAKQQMVEIAKALSF
                     NAKILVMDEPTAALTETEIDELFRIIRQLREKGVGIVHISHRLEELKQISDRVTVMRD
                     GKYIDTKRIQDVTIDQIISMMVGRTIYETAPELPENPDQDIVLEVRNINRGKVLKDVS
                     FNLKKGEILGFAGLIGAGRTELARAIFGADAYDSGEVIIKGQRVNIKSPGDAVTHGIG
                     YLSEDRKRYGLTLGMDVKENIAMAAMKNFLNWFGWVDFKKTVVRGQEMVKALNIKTPN
                     LEQQVKFLSGGNQQKVVIGKWLTADTEILIFDEPTRGIDVGAKSEIYKLLNDLAHQGK
                     AIIMISSELPEILRMSHRVVVMCEGRVTGILNAGEATQESIMKYATMRNAVVKASA"
     gene            complement(8123..>8328)
                     /locus_tag="AC16CB_15555"
     CDS             complement(8123..>8328)
                     /locus_tag="AC16CB_15555"
                     /inference="COORDINATES: protein motif:HMM:NF024770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR3343939.1"
                     /translation="VTIDDIPPPYTLEPFLTVATQPAREMGQQAAQLLFARIKREGNL
                     PCRQVVLPTEMFIRTSSGGCINA"
BASE COUNT         1979 a         2243 c         2135 g         1971 t
ORIGIN      
        1 tctccaggat cacgttcagc ggggcggatg ggtgccgcag gtagtctgcc acaatccccc
       61 gctctcgcat ctctttccca ttgggcagcg ctttgtccat tgcatctacc tggtcggggc
      121 cagagcggat atcgcagtaa aaaccgttga aagctttata accccaacgc tcttgaccac
      181 ttcgggcttg cagcgcaaga gcctgctgtg gccgcaggtc ttgaagtggg gagagatctc
      241 gatttagatc gtgtcgcggc aaagcatctt acagaaataa gcagcgaagg ccggctattt
      301 gtggatctga ctcgactaaa acgcaagtca gatcgagaca gtccacgcct gatacctggc
      361 gtatgcgtca tcccatccgg agtcgttgga aggctcccag gtagtgattt ggccagactt
      421 gcgtaccagg cttctcgctt cttcaagcga ggtaaggtga cccgttgcaa tcgcctgcac
      481 caggatgttc ccaatggcgg ttgcctccac cggcccagca accacaaccc gccctgtggc
      541 gtcggctgtc aattggttga gcaattggtt gcggctaccc ccaccgatga tgtggattac
      601 gggtaattcc atatccgata tcgcgctgag ctgttcaacc gtccggcgat attccagggc
      661 taggctctcc agaatgcaac gggtgatctc tccattcgag cgtggaacgg gttggctcgt
      721 ctcctggcaa aaactctgga tgcggcttac catcgtgccc gcttgagcat cgctggggtg
      781 taaaaagcgc ggatctgatg ggcgcacaaa ggattggagt gcctgggctt gagctgccat
      841 ctgcgtgagc tcggcatagg aataactctg cccttgaagc gcccactcac gccggcactc
      901 ctgtagcagc cacatcccca tgatattttt caacacccgg aaggtgtcat tcacaccgcc
      961 ttcattcgtc aaattaaacg ccaggctatc ctcattgatg acaggctggc gagactcgat
     1021 ccccatcagc gaccaggtgc cggaactaag atagatgtaa tctggggaac tggcaggaac
     1081 agcaaccacc gcacaagcgg tgtcgtgccc agcagtggca atcaccatca caggaccaca
     1141 atttatgtcc gcagctagtg ttggtcttaa ttttccgagc ttggtccccg ggggcacgat
     1201 ctgcccaaat atttgatccg ggatgccaag ccggttgagc atcgagcgag cccaatctct
     1261 ctcgagcggg gtgtaacact gcgtggtgga cgcgatggtg aactcactgg tcttttctcc
     1321 tgaaagccag aagttgaaca gatcaggcat gttcaagaac gtgcgcccag ccgccaggag
     1381 aggggaatcc gctttggcca tggctaggag ctggtaaagg gtgttgatct gcataaattg
     1441 gataccggtc tggtgataga tctcctcacg cgagattacg gaaaaggcct gctccagcat
     1501 gccatccgtt cgccggtcgc gatagtgata tgggttaccc agcagttcat cattggcatc
     1561 cagcaagccg aaatcgacac cccaggtgtc cacccccagg ctgaccaagc tctcccctgc
     1621 ctggtggtgt gctaatgcca atccgtgctg gatgtcactc cacaggcgca ggacatccca
     1681 atacagatga ctgcccacac ggacaggtct gttatcgaag cgatgcactt cctgaagctc
     1741 aagacgattc tcttgaaggc ctcccaggat gacgcgcccg ctctcagccc ctagatccac
     1801 agccaggaaa tttggatagg acatcaagta gttttactaa tacaacggac caaaattggc
     1861 acaggcgcgg aagtcagccc cttccaggtc ttgggtcccg aaagcaaacc atgcactggg
     1921 gcggaagatt ctttctttgg atacgttgtg catatagacc gggatccgca acatcgaggc
     1981 tagcgtgatc aaatcagctc cgatatgacc gtagctgaaa gcgccgtggt tggctcccca
     2041 ggcattcatc acagaatagg gatcacggaa taccccggac ccgtctgtgc gcggcacgaa
     2101 ccaggtggtc ggccaggtgg aattggttcg ggagtcaagc gcatcgtgaa cttcggtggg
     2161 aagatcaact acaaagcctt cagccagttg cagcaccggg cccaatccct tcaccaggtt
     2221 gagacggctt aacgtgaccg gcattccgcc gcgggtcagg aaatcggtag accagccacc
     2281 gccacggaaa tactcggtta tggatgcgca ccactcagtc acatccagac acttggcggc
     2341 ctcctcggca gtcacatccc accagggctt aatggccggt ttgccatcca cttcctgctg
     2401 accagtaccg tccagtgccg atgggccgga gttgatcagg tgcagtacgc cgttctcggc
     2461 cagcccggtc gggcgatagc cggtgacacg ctcgatggca tccgcactcc agtacgtgcg
     2521 cacatccgag aaaatctgtg cagagccagt gagcaggtgt ccaaagagca tacaagtccc
     2581 gttgagagag tcattttcag tggccacgat ataaggctgg cggattccgt tccagtcaaa
     2641 cgttgaattg agaatggctt cgagaaaatc gccgttaggc ataaaatccg tccagtgacg
     2701 ctggccttga aaaccggcgg caatcccgtt atgtccgagc gcttcttccc caaaaccgat
     2761 ttccgccaaa cgggggttgc caaccatcag gtcgcgggcg atcatcgcca tcttgacgga
     2821 tacttcccaa tctttatctt tctgctcgcg gctgcgttgc tgatcgaggg gattggtatc
     2881 ctgaccttcc ttgcaattgg ccctcaccca ttttagggct tcatcgaact caaccggatc
     2941 gtagatgttc tgattaatgc ggcggatgaa ttccaccatg tcgatggttt caacccgcat
     3001 ccctaaatag gattcgaaga aatcctgatc gacaatggaa ccggcaatgc ccatggaaac
     3061 cccgcccatc gaaacatagc tcttaccccg catggtggcg acggccaacc cggcgcgggc
     3121 aaagcgaagc agtttttcct gaacatcttc agggatgctg ttatcgccgg aatcctgaac
     3181 gtcacgccca taaatgccga aggccggcag tcccttttgg ctgtgccccg ccaggacggc
     3241 tgccaggtag accgcgccag ggcgctcagt gccgttgaag ccccaaaccg ccttgggaat
     3301 tatgggatcc atatccatgg tttctgagcc gtaacaccag cagggagtga cggtcaatga
     3361 gacgccaacg cctgcgcgcg cgaatttttc tgctgtttgg gcagcctcgg ccaccccgcc
     3421 gatgcaggta tcggcgatga cacattcaac tggcaaaccg ttggcgtggc gcaaattctc
     3481 actcaagaag gcagccacat ttctcgccat gttcatggtt tgctcttcca gggattctcg
     3541 tacgccctgg tggcgtccgt caatggtggg gcggatgccg atcttgggca tgctgcctat
     3601 aagacggttg tgagggggat tgatttgaat tgtcttcata aggttgctcc attttctaga
     3661 tatgcagtat cttgtcaatc tgattacaca ccatcatctg ccaggcgatg cataatctca
     3721 cgagtttgtt gatacagttc taaattgttc tcataataat gactgtaatt ttgccgtgct
     3781 gaataataat ggctaagatg ataccaatat catattggcg tactggatcg tctcgcccgt
     3841 gcggattaag ccaattgcct ggggaactcg taatttaaaa tcagcgtgcg attcataaat
     3901 gacagggata tctcctctgg aattagcaaa agcttgctgg atctcaggcg aattctcttt
     3961 caaaaattct tctgccatat aaacttgccc aataatgaaa ttcgatttca acgcattaaa
     4021 aacctgcagc acagttggaa tcccatccac taaagagata tctacagttt caatcatcgg
     4081 ccaatagggg aaaccgcgat ctgcgatcac caaggtatta gtatgacgca cacgggaaat
     4141 tagagataga atttgaggat taagaatgcc ggttttaatc atgctgtcga tccttccatt
     4201 ttgtctgtat taaaacaggg gctgcacgat tcttgaacag cccctgttct ttacacaagg
     4261 ttcggaagaa ccataaactt acttatagag tactgcctgg atctggggct cgtcgatgtt
     4321 ggatttgtcg taccagtaaa atccggtatc gatggtcttg ggcagctcct caccattata
     4381 ggctttgagg gcggcttcga cgcacttgta gccgatgcca acgggattct gggtgatcgc
     4441 ccccgcttcg gtaccagctc ggatcgcgtc catttgttgc tgaccagagt cgtaaccaat
     4501 gaccgtgatc tggccttcca tgcctagttc agccaccgcg ttcagaacgc cgatgattga
     4561 gccttcgttc gccccgaaga agcccttcag gtccgggtgg gcctggataa tggccttggc
     4621 caggtcagtg gatttgagct ggtcgccgcc accatactgg acgtctacaa cagtgacatt
     4681 cgggtacttg ttcttgacct ggtcaacgaa accggcaacg cggtcgatac cagtgcggct
     4741 ggtctggtcg tgggcgataa cggcaacttc gccttcgcca ccgatcagtt cgaccatctt
     4801 gtcagcagcc aaagcagccg cggcaatgtt gtctgttgca gcggtggtaa cggggatatc
     4861 gctatccaca ccagagtcga aaccgacaac ggggatgccg gcatccttgg ctttttcgag
     4921 caggttctgg aaggccttgg agtcaacggc agccagacaa atggcagcgg gtttcttatc
     4981 cagggcggtc tggaacattt caacctgctt gtcaaccatt gcttcggatt ccggaccttc
     5041 gaaggtgata tccacattca ggtctgtggc tgcctgttcg gcacccagtt tcactgcctg
     5101 ccagaactgg tgctggaagc ctttggaaat cacggggata tagggttttg cagcgggctc
     5161 agccggtgcg gctggttgag cggcgggagc gcagccagca atcacggaaa tcaatacgag
     5221 cgcgcttaac agagcaaaca acactttttt ggtgttcata aatctcctca tttagatagt
     5281 ttggattcga acaaggttaa ttactgtttg gatttgcatt ttattatcgc tgatattgtg
     5341 cctccttctt atggaatgtc gtgaataaaa tgccgtccgc aaacacttct taacgacggc
     5401 ggcgcagaat atccaggaaa acagccagga tgacaatacc acctgtcacc accatctgcc
     5461 attcctgagg tactgaaagg attcgtagtc cattggtgag cgtgctgatg ataaacgccc
     5521 caatgatggt ccccaaaatt gaaccttcac cgccgctgag ggatgttcca ccgatcacaa
     5581 ccgcagcaat agcgtccagt tcgtaacctg ccccaagggc aggttgagcc gagttcaggc
     5641 gtgaagccat cagcacgccg cccaagccgc tgaacaatcc gcacagggta tagaccgcgg
     5701 tcttccaaaa ggcaacattg acgcccgaaa ggcgagtggc ttcttcatta cttcccaggg
     5761 caaaggtata gcgcccgagg atggtgcgag acaggatcag gttggcaatg atagctgcac
     5821 caaacatgat gaatacagca ttgggcactt cgaagccggg aataatattg ccaagggcgg
     5881 aacccatcgc catttcgcgg aaagcaggcg tatcgttgaa atagatcggt ttcaactccg
     5941 agatcaccaa cgaaaggccc ttggtggcat acaacatgcc tagggtggcg ataaaaggcg
     6001 ggattttcat ccgcgagatc atgaggccgt tgaccaaccc tgccaggcct ccagccagga
     6061 tgccacatag aatgccaagt gggatgggca gatgcaagtt cgttatgagc acaccggtca
     6121 tcaccgccga aaatgtgagc accgtgccca cagaaaggtc aataccggca gtgataatga
     6181 caaaggtagc gcccaacccc agcacaccgt tgaccgcagt cgcgagcaaa atgccaacaa
     6241 agttatcaaa ggtgcggaaa aatggcgatg ccagcgaaaa acctccggct agcacgatca
     6301 gggcaccgaa agccatgata cgttgcatgg catcggaacg gaacagggtc atggcagttt
     6361 tgggttcact attcatagtt tttcttcccg tgtcacaatg aatggcgtca ttatcaggca
     6421 gatgccttca caaccgcatt acgcatggtg gcatatttca tgatactttc ctgggtggcc
     6481 tcaccagcat tcaggatacc cgtcacccgg ccttcgcaca tcaccaccac gcgatggctc
     6541 atgcgcagga tctccggcag ttcagatgag atcatgatga tggccttgcc ctggtgcgcc
     6601 aggtcgttga gcagtttata aatttcactt ttggcgccta cgtcgatacc acgagtaggc
     6661 tcatcgaaga tcaatatctc ggtatcggcg gtcaaccact tgccaatgac caccttttgc
     6721 tggttaccgc cggagaggaa tttcacctgc tgttcaaggt tgggtgtttt gatgttgagc
     6781 gccttgacca tttcctggcc gcggacaacc gtcttcttga aatccaccca gccaaaccaa
     6841 tttaggaaat tcttcatcgc cgccatggca atattttctt tgacatccat gccaagggtc
     6901 aacccataac gtttgcggtc ttcggagaga tagccgatgc catgcgtaac ggcatcgccg
     6961 gggcttttga tgttaacccg ctggcctttg atgatgacct cgccagagtc gtacgcatcc
     7021 gctccgaaaa tagcgcgcgc caattcggtg cgcccggcgc caatcaaccc ggcaaagccc
     7081 aaaatctcac ccttcttcag gttaaaactg acatctttga gcacttttcc acgattaatg
     7141 ttgcggactt caagaacgat atcctgatcg ggattttcag gcagttcggg agccgtctcg
     7201 tagatggtgc gtcccaccat catgctgatg atctggtcaa tggtcacatc ttggatacgt
     7261 ttcgtatcga tatatttccc atcgcgcata accgtcacac ggtcagagat ttgcttgagt
     7321 tcttccagcc ggtgcgaaat gtgcacaatc ccaacacctt tttcgcgcaa ttgtctgata
     7381 attctgaaga gttcatcaat ttccgtctcg gtcagggctg ccgttggttc atccatgacc
     7441 aagattttgg cattgaatga aagcgccttt gcaatttcca ccatttgctg cttggcaact
     7501 gtcaagtcag caacttttgc gactgggtcc aattgcaggt taagcatttt aaaaagacgt
     7561 tcggcgtgtt ggttggtgat tctatcatcc actataaacg ggatattgga acgcggttcg
     7621 cgaccgatga atatattctg ggccaccgtc agatgcggca tcaggttcag ttcctggtga
     7681 atgatgctga tccctagttc ctgagccata cgggggtttt gaatttcaac ctcctgacct
     7741 tcgaggtaga tgcttccagc atccttatga tagattccac cgaggatttt cataagtgta
     7801 gatttgcccg caccgttttc acctaccagt gcatgtacct caccagatcg caattcgaag
     7861 cgacattggt tgagcgcatg gacgccagga aatcgcttct cgatcccctc catgagaatt
     7921 aacactttac tcataaacca acctttttgt cacgaatttg tcactcgatc gcgttgcgaa
     7981 aatatgctcg ttttgtagaa tttctacacc gcaagttggc aggcgcatcg tattacaaaa
     8041 aaccggttct ttaagaatcg acgtggcgga ccactcctaa atccgggact ttccacggca
     8101 actcccaacg acccaaaaaa cttcaagcat taatacaacc tccgcttgaa gttctaatga
     8161 acatttcggt tggcaagacg acctgtcggc aagggagatt gccttctctt ttaattctcg
     8221 caaataggag ttgggctgcc tgctgtccca tctcacgcgc tggctgggtt gctacagtga
     8281 gaaacggctc aagagtatag ggtggaggaa tatcatctat ggtaacta
//
LOCUS       DBNGSX010000522         2719 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1715816, whole genome shotgun sequence.
ACCESSION   DBNGSX010000522 DBNGSX010000000
VERSION     DBNGSX010000522.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1715816"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1583)
                     /locus_tag="AC16CB_15560"
     CDS             complement(<1..1583)
                     /locus_tag="AC16CB_15560"
                     /inference="COORDINATES: protein motif:HMM:NF044618.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343940.1"
                     /translation="MNRPWIITVVIAILVLLVICCICLVLTFGAGALLFLNVESSRSV
                     NVIEPTPLITQVVNTPTVEASSTLRPTKAPPSPEPEQPAPETKPTPSTPAIAASDPKT
                     TLKILSDTLVPINDPADLAKRLEGKGDIPPTLPPPSVPLQVGDRQSFWVLDTDTNENF
                     RVDATLRYITDHAYFWIENNVDNNPRHLRTLAETFENKIYPTNREFFGSEWSPGVDSD
                     VHLYILYAGGLGSNLAGYFSSKDSYPPQIQEYSNGHEMFVFNADGTGLNDEYSFGVLA
                     HEFQHMIHWYQDRNEETWLNEGFSDLAMFLNGYSIGGHDFLYADDPDLQLNDWPNDSS
                     QTAPHYGAAFLFVNYFLNRFGEQATQALVADPDNGLESIDKVLSELGQIDPLTGGSVR
                     ADDVFMDWLLTSYIQDRRVGDGRYTYLDYPRAPQPYEADEVRNCTNSPQKRQVSQYGA
                     DYILINCRGNFTLRFDGSEEVGVLPENPYSGAYAFWSNKGDESDMTLTQEFDFTDHSG
                     PLTLSYWTWYDIEKDFDYL"
     gene            complement(1580..>2719)
                     /locus_tag="AC16CB_15565"
     CDS             complement(1580..>2719)
                     /locus_tag="AC16CB_15565"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR3343941.1"
                     /translation="ALFTLGIGTAMYFTVSLYSSKEDQLRASLRWLYIGFSVALLWGS
                     LQAVYIIQFDSAYFHSLAKLQSFISSRKLFTTRVSGMTYEPNWFAEQISFLLMPWLLA
                     SVLSGYSAFRWRWRWLTIEWFLLLWAMAVLIFTFSRAGLAILFLLAFTGFLFFRGQRD
                     SKPDTQLRGRRLWVRRLLEAGVVVAALIGLIFFASLKNSFFARIWSYWRDVKNASLEG
                     YFEYLGFGARFRYSDTAYRIYEAHPVLGVGLGNYAFYFPDMMPDEPLATSPELLRLIV
                     PEGNRERLITPKNFYLRLLAETGLVGMAAFAAFFAAVFGCALFLWLSSDREQRFWGRA
                     GLLGLIAFLPSAMSFDSFAIPNMWVVFGLITSAAWVFSKKETSNS"
BASE COUNT          688 a          706 c          766 g          559 t
ORIGIN      
        1 tataaataat cgaaatcctt ctcgatatcg taccaggtcc aatagcttaa cgtgagcggc
       61 ccgctatggt cggtgaagtc aaactcctgt gtcagagtca tatccgactc gtcgcctttg
      121 tttgaccaga aagcataagc gccggaataa ggattttctg ggagcacgcc cacctcttcc
      181 gagccatcga agcgcaaggt gaagttccca cggcagttta tgagaatata gtccgcgcca
      241 tactggctca cctggcgctt ttgcggcgag tttgtacagt tgcgcacctc gtcggcctca
      301 tagggttgcg gggctcttgg gtaatccaga taggtgtacc gcccatcgcc cacacgccga
      361 tcttggatgt aagacgttag cagccagtcc ataaagacat cgtcggcgcg cacgctcccc
      421 ccggtgagcg ggtcaatctg tcccagctcg cttaagactt tatcgatgct ttccaggcca
      481 ttgtctgggt cagccaccag agcctgggtg gcctgctcgc caaagcggtt taagaagtaa
      541 ttgacaaaca agaaagctgc tccgtaatgg ggggcggttt ggctggaatc gttaggccaa
      601 tcatttagct ggagatcagg gtcatcggcg tagagaaaat catgaccgcc aatgctgtac
      661 ccgttgagga acatggccaa gtccgaaaaa ccctcgttca accaagtctc ttcattgcga
      721 tcctggtacc agtggatcat gtgctggaac tcatgggcta gcacgccaaa ggaatactcg
      781 tcattcagac cggtgccgtc ggcgttgaag acgaacatct cgtgaccgtt ggaatattcc
      841 tggatttggg gagggtagga gtccttggat gagaaataac cggcgaggtt gctccccaag
      901 ccgcccgcgt aaaggatgta gaggtggaca tcgctatcca cgccaggaga ccattcgctg
      961 ccgaaaaact cgcggttggt cgggtaaatt ttattttcaa aggtttccgc cagggttctc
     1021 agatgacggg ggttattgtc cacattattt tcaatccaaa agtatgcgtg atccgtgata
     1081 tagcgcaggg tcgcatccac ccggaaattc tcgttggtgt ctgtatccaa gacccagaag
     1141 ctttgccggt cacccacctg gagcgggacg gaggggggcg gcagggtggg tgggatatcc
     1201 cccttgccct ccaggcgctt agccagatct gccggatcgt tgatgggcac cagggtatcg
     1261 cttaatatct tgagcgtggt tttagggtcg ctggccgcga tggcaggcgt gcttggggtt
     1321 ggctttgtct cgggtgcggg ctgctcgggc tctggggagg ggggcgcttt tgtcggacga
     1381 agcgtggagg aggcctcgac ggttggtgtg ttaactactt gggtaatcag cggcgtaggc
     1441 tctatcacgt tgacactacg ggaagattca acgttcaaga aaagcaatgc accggcgcca
     1501 aatgtcagaa ccaggcagat gcagcaaatt accaataaaa ccaagatcgc tatgacgaca
     1561 gtgataatcc agggacgatt catgaattag atgtttcctt ttttgagaat acccaggccg
     1621 cggaggtgat caagccaaac accacccaca tattcggtat ggcaaacgaa tcaaaagaca
     1681 ttgctgacgg caaaaaggcg atcaatccaa gcaggccggc cctgccccaa aaccgttgct
     1741 cccgatctga tgaaagccat agaaagagcg cgcagccgaa aaccgctgca aagaaagcgg
     1801 cgaaagccgc catgccgacc agcccggttt cggccaacag gcgcaggtaa aagtttttgg
     1861 gtgtgatcaa tcgctcgcgg ttgccttcgg ggacgataag ccgcagaagc tcgggtgagg
     1921 tcgccagggg ttcatcgggc atcatatccg ggaaatagaa ggcgtaattc cccaggccaa
     1981 ctcccagcac agggtgcgcc tcgtagatac ggtaggcggt gtcactgtaa cgaaagcgcg
     2041 ccccgaatcc caggtattca aaataacctt ctagactggc attcttgaca tctctccagt
     2101 agctccaaat gcgagcgaag aaagagtttt tcaggctggc aaaaaatatc aggccaatta
     2161 aagccgctac cactacaccg gcctccaaca ggcgcctcac ccagagcctg cgcccgcgca
     2221 gctgtgtatc gggctttgag tcccgctgcc cgcggaagaa taaaaagcca gtgaatgcca
     2281 acaggaagag gatggctagg ccagcgcgtg aaaaagtaaa gatcaacaca gccatagccc
     2341 acaagagcag gaaccattcg atggtaagcc aacgccagcg ccagcggaag gcactgtagc
     2401 cagacagcac cgaagccaat agccatggca tcagcaggaa actgatttgt tcggcgaacc
     2461 agttcggctc ataggtcata cccgatacgc gggttgtgaa cagcttacgc gacgagataa
     2521 aggattgtag tttcgccagg gaatggaaat aggcggagtc gaactggatt atatacaccg
     2581 cctgcaaaga accccacagc agtgccacag aaaagccgat atacagccat cgcagcgagg
     2641 cgcgtaactg atcctccttg cttgagtata gcgaaaccgt aaagtacatg gctgtgccga
     2701 tgcccagagt gaagagcgc
//
LOCUS       DBNGSX010000523         4224 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1438265, whole genome shotgun sequence.
ACCESSION   DBNGSX010000523 DBNGSX010000000
VERSION     DBNGSX010000523.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1438265"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="AC16CB_15570"
     CDS             complement(<1..893)
                     /locus_tag="AC16CB_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3343942.1"
                     /translation="MAKKYLVTGGAGFIGSNYVDRLLERGGQVSIYDNLSRAGAELNI
                     QWLQAKHGAHAFRLIVGDVRDYDLLFSTVRDADVVVHLASQVAVTTSVAEPRHDFEVN
                     ALGTFNVLEAARSSGRQPAVLYASTNKVYGGMEQVEVLEGETRYQYAHLPKGIPETYS
                     LDFHSPYGCSKGSGDQYTSDYYRIYGLPTVVFRQSCIYGPRQFGIEDQGWLAWFMSAA
                     VASRPITIYGDGKQVRDVLFIDDLLDAYDAALERIDIAAGQVYNIGGGPDHAIAVWTE
                     FGALLENLMGQKIHPKMSDWRP"
     gene            complement(920..1993)
                     /locus_tag="AC16CB_15575"
     CDS             complement(920..1993)
                     /locus_tag="AC16CB_15575"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR3343943.1"
                     /translation="MPSWLYCGMGRIETTLEVSQPPQETRQQGGEWRRILPGLVISLI
                     SLGLVFYFADIKKLGEALKLADYRFVFLSMLVTFSWLIVRGMVWRTLLQDKAQYSAVF
                     WSLNEGYLLNNLLPFRLGEFGRAFLLARKSRLEFWQVLSTVVIERILDLALAVCLLFS
                     TLPFVVGATWAREAAVFVGGAVLLGFAALYLLARNQEYAVGMFERFGRRWPILVRLGG
                     STITSLLSGLGVLNDGRRFLRALAWVLLNWLVAVVQYFVMLRAFFPEAILFWAVFSLG
                     VASLGIAAPSSPGALGVLELSIVGSLSLFGLDASTALAMAITIHLLQYLITGALGAFA
                     LARDGESLIGLYRKLRAKSSASV"
     gene            complement(1956..3755)
                     /gene="lepA"
                     /locus_tag="AC16CB_15580"
     CDS             complement(1956..3755)
                     /gene="lepA"
                     /locus_tag="AC16CB_15580"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIR3343944.1"
                     /translation="MSELIRNFCIIAHIDHGKSTLADRLLQLTGTISERDMTEQVLDS
                     MDLEREKGVTIKASAVRMQYTARDGLSYELNLIDTPGHVDFGYEVSRALAACEGALLI
                     VDATQGIEAQTLANLYMALEADLEIIPVINKIDLPSAHPDQVAEEIGSLLGTPAEKVL
                     RISAKAGINVESVLEAVVEEIPPPSGDDDTPLQALIFDSHYDPYKGVVAYIRLMEGCL
                     KHTDSLRLMVSDVVLKPVEIGVFTPYMRPAQELRAGEVGYVAPGLKTVRECRVGDTLT
                     QVTHPAAKPLPGYRKVKPMVFAGIYPVEGEEYDDLRDALEKLQLNDASLIYEPETSQA
                     LNFGFRCGFLGMFHMEIIQERLEREYDLDIIVTAPSVEYEVILNDNRVVRIDSPAQLP
                     DESQIVEIREPWMKIQIFTPTEFYGTVMELATGRRATFSDQEYPAPMRVQLNFEIPLS
                     ELIVDFFDQLKSRTRGYASLDYQFAEYRPDDLVKLEILVNNEPVDALATIVHRKDAFH
                     KGQAVISKLKDLIPRQLFVVPIQAYAAGRVISRANVKALRKDVLAKCYGGDITRKKKL
                     LEKQKRGKKRMKMVGNVEIPQDAFLAVLRHGED"
     gene            complement(3988..>4224)
                     /locus_tag="AC16CB_15585"
     CDS             complement(3988..>4224)
                     /locus_tag="AC16CB_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HIR3343945.1"
                     /translation="PLFWRLASRILLLPLLAGLAYEYIRWTANHLDSSLVQALIKPGL
                     ALQGLTTREPDLPMLEVAIAAFNAMRAYESQLVT"
BASE COUNT         1144 a         1178 c          968 g          934 t
ORIGIN      
        1 gggcgccaat cactcatttt agggtgaatt ttttgcccca tcaggttttc aagcagcgca
       61 ccaaactccg tccacacagc tattgcatga tctggaccac cgccaatgtt atagacttga
      121 ccagcggcaa tatcaattcg ctccaaggca gcatcatagg catccaacaa gtcatcaata
      181 aacagcacat cgcgcacctg tttgccgtcg ccatagatag taattggccg ggaagcgact
      241 gcggcactca taaaccacgc cagccagccc tgatcttcaa ttccaaattg ccttggtcca
      301 taaatgcagc tctggcgaaa cacgacggtt ggcagaccgt agatgcgata ataatcactc
      361 gtgtattgat ccccactacc tttagaacag ccatatggag agtggaaatc cagcgagtag
      421 gtttccggga ttcccttggg tagatgggca tattgataac gagtctcgcc ttcgagcacc
      481 tctacctgct ccatgccgcc atagacttta ttcgtggaag catacaaaac agccggctgc
      541 cggcctgaag agcgcgcagc ctccagtaca ttaaaggtcc ccagcgcgtt aacttcaaag
      601 tcatgccgtg gttcagcgac ggacgtagtt actgctacct gtgaagctaa gtgtacgacg
      661 acatcagcat ctctcacggt agaaaacagt aaatcatagt ctcgaacatc tcccacgatt
      721 aatcgaaagg catgtgcccc atgctttgct tgcaaccatt gaatgttcaa ctccgcccca
      781 gctctagaga gattatcgta gatactcacc tgcccaccac gttccagcag gcgatctaca
      841 taattagagc cgatgaaacc tgcaccgccc gtcactaaat attttttagc cataggatat
      901 attcatcccg tgcaagagtt taaacgcttg cgctactctt ggcgcgcaat tttcgataca
      961 atccaatgag cgattcaccg tcacgggcca aagcaaaggc acccaaagcg cccgtgatca
     1021 agtactgtaa aagatgtatc gtgatcgcca tcgctaaagc tgttgacgca tccaaaccaa
     1081 agagagataa cgagccaaca attgatagtt caaggacgcc taaagctcca ggagaagacg
     1141 gcgcggcaat ccccaatgat gctacaccta aggaaaaaac cgcccagaac aaaattgctt
     1201 ctggaaagaa ggcgcgcagc ataacgaaat actggacgac agccaccagc cagttaagca
     1261 acacccatgc cagagcccgc agaaagcgcc tcccgtcgtt caatacaccc agcccactca
     1321 acagagatgt gatagtactc ccgcccagac ggacgagaat cggccacctt cgaccgaaac
     1381 gctcgaacat tcctacagca tactcctgat tgcgggctag gagatacagg gcggcaaacc
     1441 ccaataaaac agcaccaccc acaaagaccg ccgcctcacg cgcccaggtt gcgcccacca
     1501 cgaaaggtaa cgtgctaaaa agcaagcaca ccgcgagcgc caggtctaga atgcgctcaa
     1561 tgaccaccgt ggaaagcacc tgccagaatt ccaaacgaga tttcctcgcc agcaaaaatg
     1621 ctctgccaaa ctcccctaac ctgaaaggca gcaggttatt gagcaaataa ccctcattga
     1681 gcgaccaaaa tacagctgaa tactgggctt tgtcttgcaa taaagttcgc cagaccattc
     1741 cacgcacaat taaccaggaa aaagttacca gcatcgaaag aaagacaaaa cgatagtccg
     1801 ccagttttag tgcttcaccc agttttttta tgtctgcgaa atagaataca agacccagcg
     1861 atatcaaact gatcaccagg ccaggcaaaa ttcgcctcca ctcaccaccc tgctggcgag
     1921 tttcttgggg gggttggctg acttccaatg tggtttcaat cctccccatg ccgcaataca
     1981 gccaggaagg catcttgggg aatctcgacg ttcccaacca tcttcatgcg tttcttaccg
     2041 cgtttttgtt tctccagcaa tttcttctta cgggtaatat caccgccata acacttggct
     2101 aatacatcct tgcggagagc tttgacattg gcacgcgaga tcacccgtcc agcggcgtaa
     2161 gcctggattg gcacaacgaa aagttggcgc gggattagat ctttcagttt ggagatcacc
     2221 gcctgacctt tatggaaggc atccttgcga tggacaatcg tcgccagcgc atccactggc
     2281 tcattgttga ccaggatctc cagcttgacc aaatcatcag gtcggtattc agcaaactgg
     2341 taatccagcg aggcatatcc acgtgtgcgt gatttcagtt gatcgaaaaa gtcgacaatc
     2401 aattcagaga gagggatttc aaaattgagc tgtacccgca tcggagcagg atattcctgg
     2461 tcggaaaagg tcgcccgtcg tccagtcgct agctccatta cagtcccata gaattcggtt
     2521 ggggtgaaaa tctgtatctt catccatggt tcgcgaattt cgacaatctg ggactcatcg
     2581 ggcaattggg caggcgaatc aatacgcaca acccggttgt cattcaagat cacctcatat
     2641 tccaccgagg gcgcggtgac aatgatatcc aaatcgtatt cgcgctccag ccgctcttga
     2701 atgatttcca tatggaacat gcccagaaaa ccgcaacgaa agccgaaatt taacgcttga
     2761 gaggtctccg gctcatagat caaagaagca tcgttcagtt gtagcttctc caacgcgtct
     2821 cttaagtcgt catactcttc gccttccaca gggtagatgc cagcaaatac catgggctta
     2881 accttgcggt agcctggcag aggcttggca gctgggtggg ttacctgggt tagggtatcc
     2941 cccacgcggc attcgcgcac tgtcttcaaa ccgggggcaa catagcccac ctcgccggca
     3001 cgaagctctt gggctggacg catatatgga gtaaaaacgc cgatctccac gggtttgagc
     3061 accacgtcgc tgaccatcaa ccttaagcta tctgtatgtt ttaggcagcc ttccatcagc
     3121 cggatatagg caactactcc tttatatggg tcataatggg agtcaaaaat caaggcttgc
     3181 aatggggtgt cgtcatcccc actgggcggg ggaatttcct ccaccaccgc ttccagcacc
     3241 gactcaacat taatcccggc cttcgcagag atgcgcaaga ccttctctgc aggtgtccct
     3301 agcaggcttc caatctcttc agccacctga tcaggatggg cagacggcag atcgatctta
     3361 ttgatgaccg ggataatctc caggtcggct tctaaagcca tatacagatt ggccagtgtc
     3421 tgcgcttcaa tcccctgggt tgcgtccaca atcagtaacg cgccttcgca tgctgccaat
     3481 gcccgagaga cttcatagcc aaagtccaca tgccctggtg tgtcaatcaa gtttagctca
     3541 tagcttaggc catctctggc tgtatattgc atgcgcaccg ccgaggcctt gattgttacc
     3601 cctttctcgc gctccaagtc catgctatcg agcacttgct ccgtcatatc gcgttcggag
     3661 atagtgcccg tcagttgcag caagcggtca gccagggtag atttcccgtg gtctatatgg
     3721 gcaataatgc aaaagttacg gattagctcg gacataggca aacaataagt ataactcaaa
     3781 caacataggc tacgaaaaat acttcgaggt ctctttggga tatgacatgg gctattaaga
     3841 tgaacctggt atacaaggct gagaggccct cataccacat acaaagatcc cgctctcctt
     3901 ttaaatatcg gagacagggg ttcgtattac tctctatgaa gaagcgtaca attagctacc
     3961 ggagatacgt ccgtcaaccc acttcaacta cgtcacgagc tggctctcat aggcgcgcat
     4021 ggcgttgaac gcggcaatgg caacctcaag cataggcaaa tctggttcgc gagttgtcag
     4081 accttgcaaa gccaagccag gcttgatcaa tgcctgcacc agcgacgagt ccaagtgatt
     4141 ggccgtccag cggatatact cgtatgccaa tccagccagc aaaggcaaga gcaaaatacg
     4201 gcttgccaga cgccagaaga gggg
//
LOCUS       DBNGSX010000524         2486 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_54013, whole genome shotgun sequence.
ACCESSION   DBNGSX010000524 DBNGSX010000000
VERSION     DBNGSX010000524.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_54013"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="AC16CB_15590"
     CDS             complement(<1..812)
                     /locus_tag="AC16CB_15590"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR3343946.1"
                     /translation="MKKYPWLQLLWLLVPVLVIWSVRDAPLADILRALSSLSFAKILL
                     LAALNAIILLLVSGRWWLILRAQGHTIRYLSVAAYRLVAFGISYFTPGPQFGGEPAQV
                     YYIRNRHGISTSSALASVSLDKILELLANFGFLLVGIFAALGSGLLVELAPIQAVAWM
                     AGPFILLLAYTLAIWKGKSPLASLLTRLPQQRGAILRLTRATALAEEQISQFCQRKPL
                     VVLGASVLSILIWGVMTLEYWLALRFLGIQVDLLQAVAALTAARIAFLFPLPG"
     gene            complement(824..1738)
                     /locus_tag="AC16CB_15595"
     CDS             complement(824..1738)
                     /locus_tag="AC16CB_15595"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR3343947.1"
                     /translation="MAEHSIFGYPSQLDTLIQVNIRDFLVAFGLEKLRRGRKVLETVC
                     WLPAQRFASQMVEFDRQVGEQGLHQASRHTLKGYVSQLEIVGQQNIPASGPLLVLSNH
                     PGMTDTLALFASLDRPDLRIIAAERPFLSALSNVSRHLIYVPDDTNQRMGVVRNVVNH
                     LRRGGAALTFPAGEIEPDPYCMPGAVDSLEGWSESMAVFARLVPETSIVVAIVSGVIW
                     PASLSHPLTRLRKAQQDRERIAASLQVLGQMLSPSLRPVAVRVAFSQALPAAELINDG
                     SMTFMNAVTEQARQLIQKAPKFSEIYRG"
     gene            1961..>2486
                     /locus_tag="AC16CB_15600"
     CDS             1961..>2486
                     /locus_tag="AC16CB_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR3343948.1"
                     /translation="MKGFSISRNPRRLGLKAFSLLLVLALLTASLPVVALAAPSAATC
                     ARNYTVQSGDTLSKISVTYDVSIAELATANNLKEPYTLYIGQQLCIPGSTTTTTTTSS
                     SSSSSSSSSSSDKFSITLENDKIVVKVSGLTKNSAFVIKGMKYDRGDTSWIKFGNLKT
                     DKKGNGSVTLKLPKH"
BASE COUNT          659 a          684 c          625 g          518 t
ORIGIN      
        1 cccggcaatg ggaacaagaa agcaatgcgg gcagcggtca gggcggcaac tgcctggagc
       61 aagtctacct ggatgcccag gaagcgcaaa gccagccaat actccagggt catcacgccc
      121 cagatcaaga tggatagcac agaggctccc agcacgacga gcggtttacg ctggcaaaac
      181 tggctgatct gctcttccgc caaggcggtg gcgcgcgtca accgcaggat cgctccgcgc
      241 tgctggggta agcgagttaa caagcttgcc aggggggatt ttcccttcca tatcgccagc
      301 gtataggcca acaacaagat gaatggcccc gccatccatg ccacagcctg aatgggcgcc
      361 agctctacga gcaaaccgga tcccaaggct gcgaatatac cgaccagcaa gaagccgaaa
      421 tttgccagca actctaggat tttatccagc gatacggacg ccagcgcact ggaagttgag
      481 ataccgtggc ggttccgtat atagtagacc tgcgctggct ctccgccgaa ctgtggtcca
      541 ggtgtgaaat agctgatgcc aaaggctacc agccggtatg ccgccacgga taaatagcgg
      601 atggtgtgcc cttgggcacg caaaatcagc caccaacgac cactgactag caagaggata
      661 atggcgttga gggctgccaa tagcagaatc ttggcgaagc tcaaactgct cagggcgcgc
      721 agtatatctg ccagaggggc atcccgcaca gaccaaatga ctagcacggg aaccaggagc
      781 caaagtagtt gcagccaagg gtactttttc atgtattgct ttgctacccc ctataaatct
      841 cagaaaactt gggggctttt tggataagct ggcgtgcctg ctcggtgaca gcgttcatga
      901 atgtcataga accatcgttg atcaactcag cagcgggcaa tgcctggctg aaagccacac
      961 gcactgcgac cggtcgcaga gatggcgaaa gcatttgacc caggacctgc aagctggcag
     1021 cgatgcgctc gcgatcctgc tgcgccttgc gcaagcgagt gagcgggtga ctcagcgatg
     1081 ccggccagat gaccccactg acaatggcga ccacgatgct cgtctcaggg accaaacggg
     1141 cgaagacagc catgctctca gaccagccct ccagcgaatc aacagctcca ggcatacagt
     1201 agggatcggg ttcaatctct ccggcgggga aagtgagtgc tgcaccaccg cgtcgcaagt
     1261 ggttaacgac attacgcact acgcccattc gctgattggt gtcatcgggc acataaatca
     1321 agtgacggct gacattggac agagcactca agaaaggccg ttcggcggca ataatacgta
     1381 gatcaggacg gtccaagctt gcgaataacg ccagtgtatc tgtcattcca ggatggttag
     1441 agagcaccag cagcggtccg ctagccggga tattctgctg tcccacaatt tctaattgag
     1501 atacatatcc tttcaaggtg tgccgagagg cttggtgcaa tccttgctcg ccaacctggc
     1561 gatcgaattc aaccatttgc gaagcaaagc gctgcgctgg cagccagcac actgtctcca
     1621 agacttttcg cccgcgccga agtttctcca gaccaaaagc gactaagaaa tcccgtatat
     1681 tgacctggat taacgtgtcg agttgtgaag gatatccaaa aatgctgtgt tcagccatta
     1741 aattacacta acctcattct aaatgactaa ttataccatc tagatctcat cttagatata
     1801 tatacgccga aatatctaat attagattta tataattggt atttaatcgc tgcctcttgc
     1861 caaaactcta ataatttcct taagcgaatc ctaaaatcga gatgtgaaaa gcggaacttt
     1921 attgtggtaa aatcgtcaga gtctgaaaag gagatacata atgaaaggct tttcaatttc
     1981 tcgcaatccc cgtaggctgg gcctgaaggc attcagcctg ctcctggtgc tggcgcttct
     2041 gacagcttcc ctgccagttg tcgccctggc agccccgtct gctgcaactt gtgccaggaa
     2101 ttacaccgtc caatctggag atacgctgag caagatctca gtcacctatg atgttagcat
     2161 cgccgagctg gcgactgcca ataacctcaa agagccctac accctttaca ttgggcagca
     2221 actatgcatc cccgggagca cgaccacaac tacgaccacc tcttccagca gctcttccag
     2281 cagctcttcc agctcgtccg ataaattcag catcaccctg gaaaatgaca agatcgtcgt
     2341 aaaggtttct ggcctcacca agaactctgc attcgtaatt aagggcatga aatacgaccg
     2401 aggcgatacc tcctggatta agttcggcaa tctcaaaacg gacaaaaagg gtaacggctc
     2461 ggttacgctc aagctaccca agcacc
//
LOCUS       DBNGSX010000525         1571 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_399642, whole genome shotgun sequence.
ACCESSION   DBNGSX010000525 DBNGSX010000000
VERSION     DBNGSX010000525.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_399642"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            294..1097
                     /locus_tag="AC16CB_15605"
     CDS             294..1097
                     /locus_tag="AC16CB_15605"
                     /inference="COORDINATES: protein motif:HMM:NF012784.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIR3343949.1"
                     /translation="MEMNVVTTPESANSIDLADIERKAKFTGTILKTTLAGALVDIGL
                     EVPGVIHISQLSEQPTNRVEDVVNVGQSVEVWVKRVDAKKKRIDLTMVKPLDLEWREI
                     TKGLNLKGKVTKLEKYGAFVDIGAERPGLVHISEMTHGYIKSPSDVVKEGDEIEVQVL
                     EVNRRKKQIKLSMKALEEKPEVFMKPVQRSKDKDREKGREKKDKQEEEIAKEVAVPTA
                     MEMALREAMDRSKTKESTGKVKAKRKSLENGELENILNRTLQNKVRSSK"
     gene            complement(1294..>1571)
                     /locus_tag="AC16CB_15610"
     CDS             complement(1294..>1571)
                     /locus_tag="AC16CB_15610"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3343950.1"
                     /translation="LGEAAGIGFLGGLGGVLLGWSAGQILNVLALAYLAGQSAQTGGL
                     PPSVAVYTPPWLPVFTLIFATLIGLLSGLYPALRAATLIPVNALKYE"
BASE COUNT          446 a          348 c          436 g          341 t
ORIGIN      
        1 tgcgcagcag ggcaattgca tatgccctga ggggcatgcg caatcccgta aaaagagtgt
       61 ttggggtgtt ttggtggggg cataccctca ccaaaacacc caattttctc tcctcagtgg
      121 tgggaaatcg cccaaatgcg attgccctat gagagggcgt aagttgcctg acaacttgtg
      181 aattctttga catatcattc ttcgtaaggt ataatcaact tttgcgactg gaattgacat
      241 cgataagttt gttccaggtg cgcgtatcac ttttatattg ggagaagttc gctatggaaa
      301 tgaacgtggt tacaacccct gagagcgcga acagtataga tcttgctgac atcgaacgta
      361 aagctaaatt tacgggcacg atacttaaaa ctacgctagc cggtgcgtta gtagatatcg
      421 gcttagaagt ccccggtgtg attcatatct cgcagctctc agagcagccg actaaccggg
      481 tcgaagacgt ggtcaatgtg ggtcaatcgg tagaagtatg ggtcaagcga gtggatgcaa
      541 agaaaaaacg cattgatctg acaatggtca aaccgctgga tctggagtgg cgcgagatta
      601 ccaaagggct taatctaaaa ggcaaggtta caaagctgga gaaatatggg gcttttgttg
      661 atatcggtgc ggaacgacct gggctggtgc acatcagtga gatgacgcat ggttatatca
      721 agtcgcccag cgatgtagtt aaagagggtg atgaaatcga agtacaggta ttggaagtca
      781 accggcggaa gaaacagatt aaactcagta tgaaagccct ggaagaaaaa ccagaggtgt
      841 tcatgaagcc agtacagcga tccaaagaca aagacaggga aaaaggtcga gaaaagaaag
      901 acaagcagga agaggaaatc gctaaagagg tcgcagtgcc tactgccatg gagatggcct
      961 tgcgtgaggc gatggaccgc tccaaaacga aggaatcgac tggtaaagtc aaggcgaagc
     1021 gaaagtccct tgagaatggc gagttggaaa atattctgaa ccgcaccctg cagaataagg
     1081 tgcgctcaag caaatagacc ccaaccatca atctcaccct ttctcccccc tagaggggag
     1141 aaagctggat aggctgcttt tgtaaattat ccccttttca ttcggaaggg gattttttta
     1201 ttggtggact tacgtaactg aaggcgaaag gtgggggttt gagcgggaat cacccaccca
     1261 gactcccgaa ctcttcaact gctcactttt gtattattcg tacttgaggg cgttgacggg
     1321 gatcagggta gctgctcgca gggctggata tagcccagat aagagtccta tcaatgtggc
     1381 aaaaatcagg gtaaataccg gcagccaggg tggcgtgtag acggccacgg aaggcggcag
     1441 tccgcctgtc tgtgcggatt gcccggccag ataggccagc gccaagacat tgaggatttg
     1501 accagcgctc catcccagca ggacacctcc cagcccgccc agaaaaccga tcccggctgc
     1561 ctcgcccaag a
//
LOCUS       DBNGSX010000526         1990 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1716139, whole genome shotgun sequence.
ACCESSION   DBNGSX010000526 DBNGSX010000000
VERSION     DBNGSX010000526.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1716139"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="AC16CB_15615"
     CDS             complement(<1..901)
                     /locus_tag="AC16CB_15615"
                     /inference="COORDINATES: protein motif:HMM:NF013799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR3343951.1"
                     /translation="MKKISYLPIVMGIIACLCLAVGAFIWATGLIDSVYAYRSPLHDN
                     PPTPGKPVGPPLTRSVVFVLVDALRLDTSLKPDIMPFLNELRSQGAWAVMHSRPPSYS
                     DPSYSVLLTGAWPDLSDGPTFNVPYEETPTLTQDNIFLAAKRNGLNTALSGYYWFEKL
                     VPKQAVDVSFYTPGEDQAADRQVVDAALPWLEGSEHNLVLIHLDQVDYAGHYEGGPRD
                     PRWDAAARRADDLIREIASQLDLSRDTLFVASDHGQIDQGGHGGHEPVLLLEPFVLVG
                     AGVIPGEYGDVNMVDVAPTLSVLV"
     gene            complement(888..1793)
                     /locus_tag="AC16CB_15620"
     CDS             complement(888..1793)
                     /locus_tag="AC16CB_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014515790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HIR3343952.1"
                     /translation="MKRAVSISLGSSDRDKRVVVQLKNELIEVERIGTDGDVGKAIRL
                     FTELDGKVDVLGVGGVDLYLRLDQREYPLYSALQLVKNVRQTPLVDGRGLKHTLERRV
                     FELAEPALGGMPYFRYAFVPVAVDRLGLAYAAESVSDRVVIGDIMIALGIPIPLMGIR
                     AYKRVARILLPAVSYFPMSMLFYGSGGAEHQPKYARYWNEADLLAGDFLFMRKYMPLD
                     LTGKTIVTNTTTPENVDFLRRRGVQAVITTTPRYEGRSFGTNMMEAALTAYAGKGRSL
                     TDEELNQLIDELDLRPNVMVFDEKD"
BASE COUNT          539 a          572 c          448 g          431 t
ORIGIN      
        1 caacaaggac agagagtgta ggcgcaacat ccaccatgtt gacatcgcca tattccccag
       61 gaatgacacc cgccccaacc aaaacaaacg gctcgagcag caaaactggc tcatgcccac
      121 catgaccccc ctggtcaatc tgaccgtgat cgctggcgac gaacaaggta tctctggata
      181 aatccaattg gctggcgatc tcacggatca aatcatcggc acggcgggcg gcagcatccc
      241 agcgtggatc gcgcggacca ccttcataat gcccggcata atctacctga tccaaatgaa
      301 tcagcaccag attgtgttcg cttccctcaa gccagggcaa ggcagcatcc accacctggc
      361 gatcagccgc ctgatcttca cccggcgtat agaagctcac atctactgct tgtttaggca
      421 ccagtttctc gaaccagtaa tagccggata atgcggtatt caatccgttg cgcttggcag
      481 ctaaaaatat attatcctga gtcagggttg gggtttcctc atacggcaca ttaaaggtgg
      541 ggccatcact caaatcaggc caagcgcccg tcaaaaggac agaataactt gggtcggagt
      601 aagatggagg ccggctgtgc atgactgccc aggcaccctg gctgcgcagc tcattcaaga
      661 atggcatgat atccggcttt aaagacgtat ccaaacgcaa ggcatcgacg agcacaaata
      721 ccacgctgcg ggtcaacggc ggtccaaccg gcttcccggg tgtcggcggg ttgtcatgca
      781 agggcgatcg ataggcatat accgagtcaa ttaggcctgt tgcccaaata aacgcaccga
      841 cagccaagca aaggcacgcg attatgccca tgacaatcgg cagatagcta atctttttca
      901 tcaaatacca tcacattcgg gcgaagatcc agttcatcga tcaattggtt taactcctca
      961 tcggtcagtg agcgaccttt gcctgcatag gccgtcaagg ctgcctccat catatttgtg
     1021 ccaaaggaac gcccctcata acgaggcgtg gttgtgatca cagcttgcac accacgccgg
     1081 cgcaagaaat caacattttc cggtgtcgtt gtgtttgtga caattgtttt gcctgtcaaa
     1141 tcaagcggca tgtacttcct catgaataag aaatccccag ccagaagatc ggcctcattc
     1201 cagtagcgcg catatttagg ctgatgctcc gcgcctccgc taccataaaa gagcatgctc
     1261 atcggaaaat aactaacagc aggtaacaaa atccgcgcca cccttttata tgctcgaatg
     1321 cccatcaaag ggatagggat gcccaatgcg atcattatat cgccgataac tactcggtct
     1381 gagacacttt cagccgcgta tgccaacccg agacgatcca cagcaaccgg cacgaaagca
     1441 tagcgaaaat aaggcattcc gccgagagcc ggttcagcaa gctcgaaaac ccgccgctca
     1501 agggtgtgct tcaagccccg cccatccacc aaaggtgttt gacgaacgtt cttaaccagc
     1561 tgtagtgcgg agtacaacgg gtattcacgc tggtccaatc gcaggtacag gtcaaccccg
     1621 cccacgccca acacgtcaac ttttccgtct agttccgtaa acaaacggat agcttttcct
     1681 acatcaccat ccgtgccgat tcgctctact tcgatgagtt cattttttaa ctgaacaaca
     1741 acgcgtttat ctcggtctga gctccccaag ctgatgctga cggcacgttt cataaccctc
     1801 caaaaggaca gatctatatc ttgccattcc aagaatgttg ctagttgact ggaagattat
     1861 attacaagtc agaccaatct cacccctcgt aagggactat catgccaaat cagctataat
     1921 tctccccgtg aaaaatggca tcacacaaaa ttacttgcgc cggcatattg ccattccaag
     1981 cgaacatgga
//
LOCUS       DBNGSX010000527        10613 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1300597, whole genome shotgun sequence.
ACCESSION   DBNGSX010000527 DBNGSX010000000
VERSION     DBNGSX010000527.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10613)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10613)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10613
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1300597"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="AC16CB_15625"
     CDS             complement(<1..494)
                     /locus_tag="AC16CB_15625"
                     /EC_number="2.3.1.-"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF046470.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HIR3343953.1"
                     /translation="MYAPATWRLIISPPSHGAWNMAADEAILEAVTRGDAPPTLRLYA
                     WQPPCLSLGHAQPWSDVDWKRLTAAGWDIVRRPTGGRAILHTDELTYAVIGPQEEPRL
                     KGGVLESYRRLSQALLEALNLLSLQAQAKPIAASDENGEAKGPVCFEVPSNYEITVNG
                     KKLLG"
     gene            complement(498..2906)
                     /locus_tag="AC16CB_15630"
     CDS             complement(498..2906)
                     /locus_tag="AC16CB_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3343954.1"
                     /translation="MTESQDNTAQIRFLLTNLGIIFIFILVLVVILATYPALFAPTPT
                     PTSTRVLSPPQTATSTPTITSTPTATRTRRPTFTATITLTPSITPTPTLTPTPTGLPT
                     LTPASPLSGDNIYRLTAWTPELADYVVSLIGNYPNTLLRQQRGENDENYYTAFYFATI
                     AQFEAILRFPTAPQAEGWRWGLAYNLALTGNPLAGEHYAELIARALNRGDTTPKDISA
                     WFEKQDPRLTLNTTPLQPPPPYLSGQLLQIEGNGSTFILLLETPSAFQTQVLASNFDF
                     ARSPEYKALARDLTGDNIQEIIIYPTTPAQSRQLSLPRIFSLAQSPPQELAFNSVSAN
                     FNIGMDYTVDWIAAQDEQGGSDLQVRSTLFPACPMELKRTYRWDGDWFQPVKTEYLVT
                     PKPTTLSFCRLLVDHAISVWGPDSAIQIMQTILPDWPPATKEDGTPFPLDARDEWRFR
                     IGIYHALLGEQDTATRAFQEIINSPSVPNSSWIARAQSWLNSYKDSQDLYRACVATEY
                     CKPAQALTYLIGSLRRSDYPSAAAFLGEAGVRFTSSGYFDFDGDDSTETWFTVRHRPG
                     EKLEFWLLAPYSQGVKAILVDLIDTNVPSLSYLDQESIPPVVLFHDNKAFRLHRLVGT
                     LEPYLTFQELPKFYRDRFKEPLKIAIQDLFNGEDPATIQKTLLDLEVYPGLLCRGTWS
                     CDEYYYMLGLASELAGDQKQAINTYVQLWSDYSKSPFTSMARLKLSGRAIGPAATQSP
                     TPQPFATGTPTPTITGTPPTRTATPTATRTATPGTPYPIDTTSPEPYPLDTPVSTTGY
                     TPYP"
     gene            complement(2899..3813)
                     /locus_tag="AC16CB_15635"
     CDS             complement(2899..3813)
                     /locus_tag="AC16CB_15635"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012904.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR3343955.1"
                     /translation="MTTAHRLSGVYAAAITPLKPDFSPDPNSLPKLLDFLAQRGCHGV
                     LLFGTTGEGPSIAPKERLLMLRAALEYRQTHPTFRLLFGTGTPSFEETVELTKTAFDL
                     GVDGVVVIPPYYFRKISDEGLLIWYSQLIQRAVPSGGAFLGYHIPPVTGIDLSFDLLR
                     RLRDAFPDRFAGIKDSSADLEHARNLGKHFGNELVVLNGTDRLFSHALQAGASGCITA
                     LANLFSPALRRVWDSHLRGEEDEQAQARLNSARALSESYPPAPPLLKMLLAHWYNFPL
                     WPVCPPLLPMPPSTAEKARVDFEPLRYD"
     gene            3894..5192
                     /locus_tag="AC16CB_15640"
     CDS             3894..5192
                     /locus_tag="AC16CB_15640"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR3343956.1"
                     /translation="MKRKHFIIVLAMLVLVACQSSVGTLESTASPAPPVGESPVKTPT
                     SLPTATPVVVTGTVRIWHSWEDPFVPALLRAIDGFNELYPSIYFDVLYVPADDLPIAF
                     EQAVQEGHAPSLLIGQAEWGPDLYDKGLVADLSDLISPDLLDTLNPAALGTGRLAVGL
                     ISVPVDIRGVVLYRNRSIIPIAPSTFEELASLAKEATRGQTLGSVLDRSFFFSGAHLI
                     GLGGSLMTEEGKPGFNNERGLDWVGLLQAYDQAGAAEFFTDNDLNMFKEGRVGFIIDG
                     TWNRTSLAETIGADNLAIDPWPIHAGGSLSGFVQAESMFLTPQALDEVHAVSLKFLQF
                     FLSPEAQSSIAEVGLIPAINGSPVNLAANQVVISDPLISQAMRALTDGVSYPANPEMG
                     IYSSQLDISLKSVFDDGVPAQQALQSAEEAINAALVTPAP"
     gene            complement(5251..6504)
                     /locus_tag="AC16CB_15645"
     CDS             complement(5251..6504)
                     /locus_tag="AC16CB_15645"
                     /inference="COORDINATES: protein motif:HMM:NF024241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3343957.1"
                     /translation="MNNISQQAKRISVTLFSAQSLASAGFITAATLNSIVGAKLGGSA
                     SWAGVPSAVYLLGGALAAYGWGYLMDVWGRRGGLVAGLFIGAVGAGLTFQAIAMHSLP
                     IFLVGMALMGIANAAVTLGRFAAAEVHPPQSRGRAISNVVLGGAVGAILGPLLVGPMG
                     ELAKSQGFDELSGGYVASGVLFLLACLTVYGGLRPDPRDLGREIAQLFPETAKLSGVT
                     RSIGQLLRQPAVKIAMIAMVLGQMVMVMVMVITAQHMRSHQHSLNAVSLVISSHTFGM
                     FGFSVLSGRLADRWGRGMVIIIGSTTLLLSCLAATISPEVLPLAVSLFLLGLGWNFCF
                     VGGSTLLADQLSPSERAPTQGFNDLFVGLASASASFSSGLIYDQSGYTIMALAGAAFS
                     LAPLLATSIWLRGLNRSPMPALPRS"
     gene            complement(6608..7831)
                     /locus_tag="AC16CB_15650"
     CDS             complement(6608..7831)
                     /locus_tag="AC16CB_15650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR3343958.1"
                     /translation="MKLDTRQQEIIQAIDAAAEAILSISHQIHSRPELGYQEIFASGL
                     LANSLEAHGYQVERGFGEIPTAFCARKGSGQGPRVAFLAEYDALPELGHACGHNVIAT
                     SALAAGIGLGAVISELDGETWVIGTPAEETDGAKVVLLERGTFNSLDAALMIHPYSGN
                     YYLSESLAMDALEITFHGKSAHAAAAPWDGKNALDAMLLTFNNVNALRQQIRPDARIH
                     GVITNGGTAPNIIPDRTVGRFYLRAKTRSYVNELVEKFKACVQGAATASGTRFEIKNY
                     ENSFDDMVNNIVLAERTRDYFVKALDSQQFQRAPESFGSADMGNVSHFVPAVHILVDV
                     TNGKTLSLHTPEFQSAVVTPYADAALLRSGKAMALTGYDVLTKKDFLEAARAEFTASL
                     GYPPGGKRTTPNLVE"
     gene            8066..8428
                     /locus_tag="AC16CB_15655"
     CDS             8066..8428
                     /locus_tag="AC16CB_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HIR3343959.1"
                     /translation="MNPQIDSTNFGSITITGTKFQHDVIIRLDGKIEKRKKKLSKAIY
                     GTSHIISIDEARHIYEDGMEQLVIGAGQFGRVKLSEEAEIFFREKAIRVAIMPTPRAV
                     EAWNSTKTGVIGLFHVTC"
     gene            8456..8713
                     /locus_tag="AC16CB_15660"
     CDS             8456..8713
                     /locus_tag="AC16CB_15660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor-like protein"
                     /protein_id="HIR3343960.1"
                     /translation="MEVRVGKVTHFYNRISVAVLELSGNLEIGDKILILGRTSELTQV
                     VTSMEIEHRKVQTVGPGMDVALKVAEPVRKGDIVYKIIDNE"
     gene            8725..>10613
                     /locus_tag="AC16CB_15665"
     CDS             8725..>10613
                     /locus_tag="AC16CB_15665"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3343961.1"
                     /translation="MSIERLLEKPGRMFRVIFDRLSLPVQMILGFVFVVVLTAVTIGV
                     PTIWLLRHDLDRQAWIVVEQGVRAADAFCAAQQKEIIDFATLTAERPTLHEFLSQDNR
                     RDLLDYLVTLKNAAAYDLIVVCDVDDKVVTATENMVSNQICLEKDIGGMYLVDSQEPP
                     QALMVAIHPIVMDSRTIGRVIVANRIDHAFATQMRAFSGLENTLLVSGRPVASSFDFD
                     LSDLSNIPHQLIAAGSPSGIERSQFKLDGQPYYAEKVPLEYLGVTVEVALLVSDIVRS
                     QQRLLWLLIGGMLGVASLVSALGVYISQQISKPLVDLAETAAEFSMGELSSPVSIEAR
                     VREAVLVGQALESARVNMLHTLTDLRREKAWTNHLLESIVEGIMTLDEENHIAFFSHG
                     AERITGWKREDVLNKSCDEFFKPVEIDLPFSQVIPSFGMRKRIVAQMAGDRVATLAMT
                     GARLTLQEAGEAHIALVFRDISEEDLIHHLLGHFMANIVHEFRTPLTALNASIELLLD
                     QEKRGDPAERHALLKGLQVSVINLQTLVDNLLESASFEAGHFRVWPRPYDLSDLVSEA
                     VRTMQPLLEKYGQRLLIRLPVDIPLVHIDPRRTAQVLVNLLSNASKYGPPDVEIILSV
                     HVENNS"
BASE COUNT         2528 a         2524 c         2998 g         2563 t
ORIGIN      
        1 cccaacagct tcttcccatt gacagttatt tcataatttg atgggacttc gaagcacacc
       61 gggcctttgg cctctccgtt ctcgtctgac gcagcaattg gtttggcctg tgcttggagg
      121 ctcagcaggt tgagggcttc cagaagcgcc tgggacaggc gacggtagct ctccagcaca
      181 ccccccttta aacgcggttc ttcctgtgga ccgatgactg catacgtcag ctcatcggtg
      241 tgcaaaatag cccgcccgcc cgtgggtcgg cgtacaatat cccatccggc ggctgtcaat
      301 cgtttccagt cgacatcgga ccacggctgg gcatgaccca gcgataggca aggtggctgc
      361 caggcataca ggcgcagtgt aggtggggcg tctcctcggg taaccgcttc aaggatagcc
      421 tcatccgccg ccatattcca cgcgccgtga gaaggtggtg agataattag tcgccaggtt
      481 gcaggagcgt acataagcta aggataaggc gtgtaacctg ttgtggatac gggagtgtca
      541 agaggatatg gttcaggtga ggtagtatcg atgggatatg gcgtgccggg ggtggcagtt
      601 cgggtagctg tgggagtggc agtccgggtt ggtggcgttc ccgtgatggt cggcgttggg
      661 gtgcctgtcg caaaaggttg tggggttggt gattgcgtgg cagcaggtcc aatagctctt
      721 ccgcttagtt tcaagcgagc catgctagtg aaggggcttt tagagtaatc tgaccacaat
      781 tgcacatagg tatttatggc ttgcttctgg tcccctgcca gttcggacgc caggccaagc
      841 atgtagtagt attcgtcgca actccaagtg ccgcggcaca acagacccgg atatacctct
      901 aggtctagca aggtcttttg gattgtggcg ggatcttcac cattgaacag atcctggata
      961 gcgattttca gcggttcctt gaagcggtca cggtaaaact tgggtagctc ttgaaaagtg
     1021 aggtatggct ctaaagtacc aaccagccga tgcagtctga atgctttgtt gtcgtggaat
     1081 aaaacaacag gcgggatgct ctcctgatcc agataggaaa gcgacggaac gttggtgtcg
     1141 atcaagtcga ctaatatggc tttgaccccc tgggaataag gcgcaaggag ccaaaattcc
     1201 agtttttcac cggggcgatg ccgcacggta aaccaggttt ccgtcgagtc gtcgccatca
     1261 aagtcgaagt aaccagagga tgtgaagcgc acgccggcct ccccaaggaa ggcagcggcg
     1321 gaggggtaat cactgcgtcg caggcttcct attaggtagg tgagggcttg agctggtttg
     1381 cagtattccg tggcgacaca agcgcgataa aggtcctggg agtctttgta actgttcagc
     1441 cagctctgtg cccgggcaat ccaagaactg ttcggcacag aaggagaatt gatgatctct
     1501 tgaaaggcgc gcgtggctgt atcctgctcg cccagcaagg catggtagat accgatgcgg
     1561 aagcgccact cgtcgcgcgc atccaggggg aaaggtgttc catcctcttt cgtggcgggt
     1621 ggccagtctg gcaagatggt ttgcataatt tgtatagccg aatcaggtcc ccatacgctg
     1681 atggcgtgat ccacgagcaa gcgacagaag ctcagcgtgg tcggtttagg tgtcaccaga
     1741 tattcagtct taactggttg gaaccaatct ccatcccaac ggtaagtgcg tttgagctcc
     1801 atggggcagg ccgggaaaag ggtgctgcga acctgcagat cagagccacc ctgctcatcc
     1861 tgtgccgcta tccagtccac ggtatagtcc atgccaatat tgaagttcgc agatactgag
     1921 ttgaaagcca actcttgcgg aggggattgc gccaagctga aaatgcgcgg caaagataat
     1981 tggcgagatt gcgccggggt tgtgggatag atgatgattt cctggatgtt atcccctgtc
     2041 agatctctag ccaacgcctt atattcaggg ctgcgcgcga aatcaaagtt gcttgcaagc
     2101 acctgggtct ggaaggcaga tggtgtttca agtaacaaga tgaaggtgct gccatttcct
     2161 tcaatctgca gcagttgacc gctgaggtag ggaggagggg gttggagggg tgtggtattt
     2221 agggtcaatc gcggatcttg cttttcaaac caagcggaga tgtctttggg ggtggtatcg
     2281 ccgcgattaa gtgcccgggc gattagctcg gcataatgct cccctgctag agggttgccg
     2341 gtcagcgcca ggttgtaggc taaaccccag cgccagcctt cagcctgggg ggctgtggga
     2401 aagcgcaata tcgcttcaaa ttgggcaatt gtggcgaaat aaaatgcggt gtaatagttc
     2461 tcatcattct cgccgcgttg ctgccggagc aaggtgttgg gatagttccc gatcaatgac
     2521 accacgtagt cagctaattc aggtgtccaa gctgtgaggc ggtaaatatt atcgccggat
     2581 aacgggcttg caggtgtaag tgtgggcaag ccggttgggg taggtgtcag ggttggggtg
     2641 ggcgttatag aaggtgtgag tgtaatagtt gctgtgaagg tgggccggcg agtgcgagtg
     2701 gctgtgggag tggatgtgat tgtcggcgtg gaggttgcgg tctgtggggg agacaagacg
     2761 cgcgtgctgg taggggtagg cgtgggtgcg aagagagccg ggtaagttgc caaaataacc
     2821 accagcacca ggataaaaat aaatattatc ccaaggttgg tgagtaaaaa gcgaatttgg
     2881 gcagtgttgt cttgcgattc agtcatagcg tagaggttcg aaatccaccc tggctttttc
     2941 ggctgtactt gggggcatcg gcagcagtgg cgggcataca ggccatagag ggaaattgta
     3001 ccagtgcgct agtagcatct tgagcaaggg aggcgcaggt ggataactct ctgaaagggc
     3061 gcgggcagag ttcaggcgag cttgcgcttg ttcatcttcc tcacccctga ggtgtgaatc
     3121 ccatacccgg cgcagcgcag gagagaataa attcgccagg gcagtgatac agccagaagc
     3181 cccagcttgt aaggcatggc tgaacaggcg gtcggtgcca ttcagcacca ccaattcatt
     3241 tccaaagtgt tttcccagat tgcgggcgtg ctcaaggtcg gcggaggaat ccttgatccc
     3301 agcgaaacga tctggaaatg cgtctcttaa ccgtcgcagc aggtcgaagg aaagatcaat
     3361 gcctgtgaca ggcgggatgt ggtagcctag gaatgcaccg ccggacggca cggctcgttg
     3421 gataagctgg ctgtaccaga tgagcagccc ctcgtcgctg atttttcgaa aataataggg
     3481 cggtatgact actacaccgt cgacaccgag gtcaaaggcg gtcttggtga gctccacggt
     3541 ctcttcgaag ctaggtgttc ccgtaccgaa gagcaagcgg aaagtggggt gagtctggcg
     3601 atactccagg gctgcccgca gcatgagcag gcgctccttg ggtgcaatcg atggaccttc
     3661 acctgtggtg ccaaacaaca agaccccatg gcaaccgcgc tgtgcgagaa aatcgagcag
     3721 cttgggcaag gagttaggat cgggagaaaa atctggcttg agcggggtaa tcgctgcggc
     3781 atatacgccg gacagccggt gagccgtggt catggggtga ttatactgtt tattgctcga
     3841 aactggtaca attatccttc aggcgaatca ataccctctc taaaaggatc aggatgaaaa
     3901 ggaaacattt tattattgtc ctggccatgc ttgttttggt cgcttgccag agcagtgtgg
     3961 gcactctcga atcaactgcc tcccccgcac cgcctgttgg cgaatccccc gttaagacgc
     4021 cgacatcatt acctacagcc acaccggttg tggtgactgg gacggtgcga atctggcatt
     4081 cgtgggaaga tccattcgtg ccggcattgt tgcgggcaat cgatgggttt aatgagctat
     4141 atcccagcat ttattttgat gtgctctatg tgcccgctga tgacctgcct atcgccttcg
     4201 aacaggctgt tcaggaaggg catgccccga gtttgcttat tgggcaggca gagtgggggc
     4261 cagacttata cgataaaggg ttggtggcag acctgtctga tctgatttca ccagacctgc
     4321 tggatacact taatcctgct gcgctgggca ccgggcgcct tgcagttggt ctgatcagcg
     4381 ttcctgtaga tattcgcggt gtggtcctgt accggaatag aagtattatc ccaatagctc
     4441 catccacatt cgaagagcta gcaagcctgg ctaaggaagc cacccgcggg cagactttgg
     4501 gttctgtgct tgatcgtagc ttctttttct ccggcgccca cctgattggt cttggtggca
     4561 gtttgatgac agaagagggg aagccaggtt ttaataatga aagaggtctt gactgggtgg
     4621 gcttgctgca agcttacgac caggcggggg cagccgagtt tttcacggat aatgatctca
     4681 acatgttcaa agagggacgc gtaggcttta tcatcgatgg aacgtggaat cggacttccc
     4741 tggcggagac aattggcgca gataacctgg caatcgatcc ctggcctatt catgcaggtg
     4801 gctctctctc tgggtttgta caggcagaga gcatgtttct tacccctcaa gcattggatg
     4861 aagtccatgc cgtatcattg aaatttttac agttttttct ttcacccgaa gcgcaatcct
     4921 ctattgcaga ggttggcttg atcccagcca tcaacggctc tccggtcaac ctggctgcta
     4981 atcaggtcgt catctcagac ccactcatct cccaagccat gcgcgccctg acggatggcg
     5041 tcagctatcc agcgaatccg gagatgggca tttactccag ccagttggat atttccttga
     5101 agtctgtctt tgacgatggt gtgccagcac agcaggcgtt gcagagcgcc gaagaagcga
     5161 tcaatgctgc tttagtgaca cccgcaccgt aacctattcc cgctggatcc aggatgtcat
     5221 ctgtgattgg gggttataac gtgctgcccg ttatgatctt ggtaacgctg gcattgggct
     5281 gcgattcagt cctcttagcc agattgaagt agccagtaaa ggggcgagcg agaatgctgc
     5341 tccagccaat gccataatgg tgtagcctga ttggtcgtat atcaacccac tgctgaagct
     5401 ggcggatgca gacgctagac caacaaacag gtcattgaaa ccctgggtag gtgcacgctc
     5461 agacggcgag agctgatcgg ccagcaaggt ggagccgcct acaaaacaaa agttccagcc
     5521 aagacccaaa agaaaaagcg ataccgccaa tggcaggact tcgggagata ttgtggctgc
     5581 caggcaggat agtaagagcg tggttgaacc gataataatt accatgccgc gaccccagcg
     5641 atctgccagt cgaccagata aaactgagaa accaaacatc ccaaatgtat gggatgaaat
     5701 gaccagagat acagcattaa ggctatgctg gtgggagcgc atatgctggg cggtgatgac
     5761 catcaccatt accatcacca tttgtcctag caccatagca atcatggcga ttttcactgc
     5821 cggctggcgc aggagctggc caatcgaccg tgttaccccg ctgagttttg ccgtctcagg
     5881 gaagagttgg gcaatttcac gtcctagatc acgaggatca ggtcgtagcc cgccgtaaac
     5941 cgtcaagcag gcaaggagga acagcacacc gctggctaca tagccacctg atagctcatc
     6001 aaacccctgc gatttcgcga gttcacccat tgggcctacc aaaagcgggc ccagtattgc
     6061 tcctactgcc ccgcccaata caacattcga gattgcccga ccacgagatt ggggagggtg
     6121 tacttcagca gcggcaaaac gtcccagagt tacagcagca tttgcgatac ccatgagtgc
     6181 catgccgacc aagaaaattg gcaatgagtg catagcgatg gcttgaaaag tcagcccagc
     6241 acccactgcg ccaatgaaca ggccagcaac cagcccaccc cgcctgcccc agacatccat
     6301 cagataaccc cagccgtagg ctgccagcgc acctcccagg agataaaccg ccgaagggac
     6361 acccgcccag gatgcactcc cacccaattt ggcgcccaca atcgagttga gtgtggctgc
     6421 ggtgatgaaa ccagcggatg ccaggctttg ggcagaaaat agtgtcacgc ttatacgttt
     6481 agcttgctga gagatgttgt tcatcggatt tgggtttact gtgaggataa ggaggcaatt
     6541 ggaatataga aatggatttc ttgaagtaat tataaccttt tttactaaat agataagaat
     6601 actaaaatta ttctacgaga tttggggtgg tccttttccc tcctggtgga taccccaggc
     6661 tggcggtgaa ctcggcgcgg gcagcttcca gaaaatcctt tttcgtgagc acatcatagc
     6721 cggtcaaagc catcgccttg ccagatcgaa gaagggcggc gtcagcgtaa ggcgtgacaa
     6781 ccgccgactg aaattccggg gtgtgcaatg agagcgtttt tccattggtc acatcgacca
     6841 ggatgtgtac cgcaggtacg aagtgagaga cattgcccat atccgctgag ccaaaacttt
     6901 ccggggcgcg ctgaaattgc tggctgtcca gagccttgac aaagtaatcg cgagtacgtt
     6961 cagccagtac gatgttgttc accatatcat caaagctgtt ttcgtaattc ttaatctcaa
     7021 aacgcgtacc ggaggctgtg gctgcacctt gaacgcatgc cttgaatttc tcgacaagtt
     7081 cattgacata gctgcgtgtt ttagcccgca agtaaaatcg tcctacagta cggtctggga
     7141 taatattggg tgcagtgccg ccgtttgtga tcaccccatg gatgcgcgca tctggtcgga
     7201 tttgctggcg caaagcatta acattgttaa atgtcaggag catagcgtcc agggcgtttt
     7261 ttccatccca gggggcggct gcggcatgcg ctgatttgcc gtggaatgtt atctcgaggg
     7321 catccattgc caaggattcg gacaggtaat aattccccga atacggatgg atcattaagg
     7381 ctgcgtccag cgaattgaaa gtccctcgtt cgagcaatac gacctttgcg ccatcggttt
     7441 cttcggcagg tgtaccgatc acccaggttt cgccatccaa ctctgaaatc accgctccca
     7501 gaccgatccc agcagccaag gcagacgtgg caatcacatt atgtccacag gcatggccta
     7561 gctctggtaa tgcatcgtat tctgccaaga aagctacccg tggaccttgc ccacttccct
     7621 tgcgagcaca aaatgcggtg gggatttccc caaagcctct ttcgacctgg tacccatgcg
     7681 cctccaggga attggctaat aaacccgatg caaaaatttc ctggtaacct aattcgggcc
     7741 gactgtgaat ttggtgagaa atagacagga tggcttccgc agcggcatcg atcgcttgga
     7801 taatttcttg ctgtcgagtg tctaatttca tcgattatcc ttttgaaaaa ttagaatgat
     7861 aaatggcgta aacgatactg gcagccgacg aatggaaagc ggatctattt taatcgcttt
     7921 tcgaacccga ttttcgtttt ttctcattga gatatatctt gggtggggct ggagtttaga
     7981 gcatggggtg aatgagacag tcttttatag aaaatgctga aataagtcac ttgattttgt
     8041 tcaaattttc acttatgcta taagtatgaa cccgcaaatt gactcgacca attttggctc
     8101 tatcacgatc actgggacaa aattccagca cgatgtgatc atccgtctgg atggcaagat
     8161 cgaaaaacgc aagaaaaaac tctccaaggc gatctatggc acatcgcata tcatctcgat
     8221 tgacgaagcc aggcatattt atgaagacgg tatggaacag cttgtgatcg gcgctggtca
     8281 attcggaagg gtcaaactct cagaggaggc tgaaatcttt ttcagggaaa aggccatccg
     8341 ggtagctata atgcccacac cgagagcagt tgaagcctgg aatagcacaa agacgggtgt
     8401 catcggtctt ttccatgtaa cgtgttgacg attagggaga agggagggtg aagcgatgga
     8461 ggtacgcgtc ggtaaagtaa cccattttta caacaggata agcgtggctg ttttggaact
     8521 ttcagggaac ttagagatcg gcgataagat cttgatcttg gggaggacat cggaactaac
     8581 ccaagtcgtt acttcgatgg agatagagca tcgcaaagtg caaacggttg gccctgggat
     8641 ggatgttgct ctgaaagttg cagaacctgt acgcaagggg gacattgtgt acaagatcat
     8701 tgataacgaa taaacgaggc tgttttgtct atcgagcggt tgctggaaaa gccaggccga
     8761 atgttcaggg taattttcga taggctgagc ttgccagttc agatgatact gggttttgtt
     8821 ttcgttgttg tcctgactgc cgtgacaatc ggcgttccta cgatatggct gcttcgtcat
     8881 gatttagacc gacaagcgtg gattgtggta gagcagggag tgcgcgccgc agatgctttc
     8941 tgcgcggctc agcagaaaga gatcatcgac tttgccacct tgactgcaga acgtccaacg
     9001 ttgcatgagt tccttagtca agataaccgt agggatcttt tggattattt ggtgaccctt
     9061 aagaatgctg cagcatatga tctgattgtg gtttgtgatg tggatgacaa agttgtcacc
     9121 gcaacagaga atatggtctc caatcagatc tgtttggaaa aagacattgg tggaatgtac
     9181 ctcgtggata gtcaggaacc ccctcaggca ttgatggttg ccatccatcc tatcgtcatg
     9241 gatagccgta ctatcgggcg tgtaattgta gcaaaccgta tagaccacgc gtttgctacc
     9301 caaatgcggg ctttcagcgg gttggaaaac acacttttgg tgagtgggcg acctgtcgcc
     9361 tcgagctttg actttgactt atctgatcta tccaacattc ctcatcaact gattgctgca
     9421 ggctcaccaa gcgggattga gcgaagtcag ttcaaattgg acgggcaacc ctattatgcc
     9481 gaaaaggtac cgctggaata tctgggtgtg accgtagaag tcgccctcct ggtctctgat
     9541 atcgttcgct cgcagcagcg cctgctgtgg ttgttgattg ggggtatgct tggtgtagct
     9601 tcattggtct cggctctggg tgtctacatc tcccagcaga tcagtaaacc tctagtagac
     9661 cttgcagaaa cggcagctga gttcagcatg ggagaactga gttcgcctgt atcgattgag
     9721 gcgcgtgtgc gcgaagctgt cttggttggt caagctttgg agagtgcccg tgtaaacatg
     9781 ttgcatacat tgaccgactt gaggcgcgaa aaagcatgga caaatcacct gctcgaatcc
     9841 atcgtcgaag gaatcatgac cttagatgaa gagaatcata ttgctttttt ctcccatgga
     9901 gcagaacgga tcactggatg gaaacgcgaa gatgtactga acaaatcgtg tgacgaattt
     9961 ttcaaaccag ttgagatcga cctcccattc agtcaggtga taccctcctt tgggatgcga
    10021 aaaagaattg tcgcccagat ggctggtgac cgtgtagcta ccctagcaat gactggtgcc
    10081 cgccttactc tccaagaggc aggtgaagca catattgcct tggtgtttcg cgatatcagt
    10141 gaagaagact tgatccatca ccttcttggg catttcatgg caaatattgt tcatgagttt
    10201 cgcactccac ttacagcgct caatgcttct attgagctgc tcctggatca ggagaagcga
    10261 ggagaccctg ccgagcggca tgcgctgctc aaaggcttgc aggtgagcgt gattaatctt
    10321 caaacgttgg tggataatct cctggaaagt gccagttttg aggcagggca ttttcgtgta
    10381 tggccacgtc cttatgatct ctctgattta gtttctgaag ccgtgcgcac catgcagcct
    10441 ctgctggaaa aatatggtca gcgccttctg ataaggttgc cagtcgatat cccattggtc
    10501 catattgatc cacgccggac cgcgcaggtg ttggtgaact tgctttccaa tgccagtaaa
    10561 tatggcccac ctgacgtcga gattatattg agcgtgcatg tggaaaataa ctc
//
LOCUS       DBNGSX010000528         3249 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1300708, whole genome shotgun sequence.
ACCESSION   DBNGSX010000528 DBNGSX010000000
VERSION     DBNGSX010000528.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1300708"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..2158
                     /gene="ppk1"
                     /locus_tag="AC16CB_15670"
     CDS             17..2158
                     /gene="ppk1"
                     /locus_tag="AC16CB_15670"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HIR3343962.1"
                     /translation="MVATAEQVTDIKDLPVDLNDPKLYINRELSLLEFQRRVLEEARD
                     TSNPLLERVKFLAILGSNMDEFYMVRVSGLKKQLAAGIIDLPPDGLTPAEQLAAIRKM
                     ALQLMTQSRECLTKDIMPALNKAGIHIMDYKDLDERQKSNVKAYFDEVVFPVLTPLAF
                     DPGHPFPHISNLSLNLAVLICDETQRKHFARIKVPNTLPRLLPIKRSSGGVRKDGTVP
                     YHHYFVWLEQVIAANLDALFPGMEILEAHPFRVTRDADMEIQELEADDLLESMELNVR
                     QRRFGSVVRVTLNDDMPAHVREILIKNLEVERNDIYTLFGPLGLSDLMQLYHIDRHDL
                     KDAPFVPATPLPLKSEQYDGNLFAAIRKEDILLHHPYESFLPVVDFLKMAARDPDVLA
                     IKQTLYRVGRNPPVVQSLLEAVEKGKQVAVLLELKARFDEESNIGWARKLESAGVHVI
                     YGLLGLKTHSKVALVVRKEGEGIRRYIHLGTGNYNAVTAQQYEDMGMFTCDDEIGADA
                     TDLFNYLTGYSNKKEYRKLLVAPINLRERMQALIQREIEHQKNGEKGHLIFKMNSLVD
                     KPIIQELYQASQAGVKVDLLVRGICCLRPGLNGISENIQVTSILGRFLEHSRIFYFRN
                     AGKEEVYLGSADLMPRNINRRVEVLFPVQERHMIRYLRDDVLATCLADNLRARRMLPD
                     GTYERLKPPSKAKSVDSQAWLLNHHLHPQKG"
     gene            2162..>3249
                     /locus_tag="AC16CB_15675"
     CDS             2162..>3249
                     /locus_tag="AC16CB_15675"
                     /inference="COORDINATES: protein motif:HMM:NF017085.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HIR3343963.1"
                     /translation="MPDNLLTREQRQYLEKLVEETADESLSRRARVLLLYDDQLATRH
                     VAEGAGLSRGRTRYWRRQFQGRGMAIFSRDQKFPDLRPTEPKDGEEQAGEPAQGKATR
                     PKKTKDGGGAPTDTRSVLDEFAAATKALGSPGVLSDDPLAEAGRKVWRYHFAMMLLHE
                     EGTLLGEDIEELHDMRVATRRMRAAFDVFGSAFAPKAIKRHLRGLRATGRALGQVRDL
                     DVFIDKAQRYQVSLSPEHQRDLEPLLEAWARDRADARREMIAYLETSQYRDFKEKFYK
                     FLSTPGAGVLPRSDNLAIPYLVREAAPVLIYNRLAAVRTFDTILDQAALEQFHALRIE
                     FKKLRYTLEYFREVLGSEAKTVISEIKS"
BASE COUNT          755 a          884 c          914 g          696 t
ORIGIN      
        1 attaaggaag aagaatatgg tcgcgactgc tgagcaagtt acagatataa aggatttacc
       61 ggttgacctg aacgatccca agctgtatat caaccgggag ttaagcctgc tggagttcca
      121 gcggcgggtg ctggaggaag cgcgcgatac cagcaatccc ttgcttgaac gggttaagtt
      181 cctggcaatc ctgggatcga acatggatga gttctacatg gtgcgcgtat ccggccttaa
      241 aaagcagctt gctgcgggca tcatcgacct gcctcccgat gggcttaccc cagccgagca
      301 gctcgctgct atccgtaaaa tggccctgca attaatgacc caatcgcgcg agtgcttgac
      361 caaagacatc atgcccgccc tgaataaagc tggcatccac attatggatt ataaggacct
      421 cgatgagcga cagaagtcga acgtcaaggc ctattttgat gaagtggtct tcccggtgct
      481 tactccgctg gcctttgatc ctggacatcc ttttccccat atctcaaatc tgagcctcaa
      541 tctggcggtc ttgatctgcg acgaaaccca aagaaaacac ttcgcccgca tcaaagtgcc
      601 caataccctg ccgcgcttat tgcccatcaa gcgctcgtcc ggcggtgtgc gcaaagacgg
      661 gactgtgcct taccaccatt attttgtctg gctggagcaa gtcattgccg ccaacctgga
      721 cgcacttttc cccggcatgg agatcctgga agcgcaccca tttcgcgtga cccgagatgc
      781 tgatatggag atccaagagc tagaggccga cgacttgctc gaatcaatgg agctaaatgt
      841 ccgccagcgg cgctttgggt ctgtggtacg cgtcacgctt aacgatgata tgccagccca
      901 cgtccgcgag attttgatca aaaacctgga ggtagagcgt aacgatatat ataccctctt
      961 cggcccgctg ggtttgagcg acctgatgca gctctaccat atcgatcgcc atgatctcaa
     1021 ggatgcgccc tttgttcctg ctactccgct gccgttgaaa tctgagcagt atgatggcaa
     1081 cctgtttgcc gcgatccgta aggaagatat cttattacac catccctacg agtcttttct
     1141 gccagtggtg gatttcctaa agatggccgc ccgcgacccg gacgtcctgg cgatcaagca
     1201 aacgctctac cgtgttggtc gtaatccgcc agtagtgcaa tccttgctgg aagcggtgga
     1261 aaaaggcaaa caggtggcag tgttgctgga gctaaaggcg cgcttcgacg aggagagcaa
     1321 tataggttgg gcgagaaagc tggagtcggc cggagtgcat gtgatctacg gcttgctggg
     1381 cttgaagacc cattccaagg tcgccctggt ggtgcgcaag gagggggagg gcatccgtcg
     1441 ttacatccac ctggggacgg gcaactacaa cgccgtcacc gcccagcagt acgaggacat
     1501 gggtatgttc acctgtgatg acgagatcgg cgcggatgcc accgacctgt ttaactacct
     1561 gacaggttac tcgaacaaga aagaatatcg caagctattg gtggcgccaa tcaacctgcg
     1621 cgagcggatg caggcgctca tccagcggga gattgaacat cagaagaacg gcgagaaggg
     1681 tcatctgatc tttaagatga actccttggt agataagccc attattcaag agctttacca
     1741 ggcctcacag gctggtgtga aggtggatct gctggtgcgc ggcatatgtt gtttgcgacc
     1801 gggtttgaac ggcatcagtg agaatatcca ggtcaccagc attttgggac gctttttgga
     1861 gcatagccgc atcttctact tccgcaatgc agggaaggaa gaggtctatc tagggagcgc
     1921 ggacctgatg ccgcgtaata ttaaccggcg cgtggaggtg ctctttccag tgcaggaacg
     1981 ccatatgatc cgctacctgc gcgatgacgt gctggcgact tgtctggcgg ataatttgcg
     2041 agcgcggcgc atgctacctg acggcactta tgagcgcctc aagcctcctt ccaaagcgaa
     2101 gtcggttgat tcacaagctt ggctgttgaa ccatcatcta cacccgcaaa aaggctgatc
     2161 aatgcccgat aacttactaa cccgagagca gcggcagtac ctcgaaaagc tggtagaaga
     2221 aacagccgac gagagcctca gccgccgcgc tcgcgtattg ttgctatatg acgaccagct
     2281 tgccactcgc catgtggcgg agggggcggg cctgtctcgc ggcaggaccc gctattggcg
     2341 caggcagttt caaggccggg ggatggccat cttttcccgc gatcaaaaat ttccagacct
     2401 ccgacccacc gaacccaagg atggcgagga gcaagctggc gaaccggcac agggtaaggc
     2461 aacccgccca aaaaaaacca aagatggcgg aggagctccc accgatacta gaagcgtgct
     2521 ggatgaattt gccgccgcga ccaaagccct gggtagtccg ggggtgttgt ccgacgaccc
     2581 actagctgag gccgggcgta aagtgtggcg ctaccacttc gccatgatgc tgctccatga
     2641 agaaggcact ctgctgggcg aggacattga ggagttgcac gatatgcgcg tggctacccg
     2701 gcgcatgcgg gccgccttcg acgtgtttgg aagtgccttt gcacccaaag cgatcaagcg
     2761 gcatctcagg ggcttgcgcg ccacaggccg tgccctcggt caggtgcgcg atctagatgt
     2821 ctttatcgat aaagcccaac gctaccaggt aagcctctcc ccagagcacc aacgagattt
     2881 ggaaccactg ctggaagcct gggcgcggga tcgtgctgat gcccgccgcg agatgatcgc
     2941 ctatttggag acaagtcaat accgggattt caaagagaaa ttctacaagt tcctttccac
     3001 gcctggggct ggagtcctgc cgcgctccga taatttagcc attccctatc tggtgcgaga
     3061 agcagcgccc gtgttgattt acaaccgttt ggctgccgtg cgaacttttg acactatcct
     3121 tgatcaggct gccctggagc aatttcacgc tctgcgcatc gagttcaaga agctgcgcta
     3181 caccctggag tacttccgcg aggtcttagg gagcgaagcc aagactgtca tcagcgaaat
     3241 caagagtat
//
LOCUS       DBNGSX010000529         2014 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_954646, whole genome shotgun sequence.
ACCESSION   DBNGSX010000529 DBNGSX010000000
VERSION     DBNGSX010000529.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_954646"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..827)
                     /locus_tag="AC16CB_15680"
                     /pseudo
     CDS             complement(201..827)
                     /locus_tag="AC16CB_15680"
                     /inference="COORDINATES: protein motif:HMM:NF019339.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(840..>2014)
                     /locus_tag="AC16CB_15685"
     CDS             complement(840..>2014)
                     /locus_tag="AC16CB_15685"
                     /inference="COORDINATES: protein motif:HMM:NF036774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3343964.1"
                     /translation="LGLEYENLGEHQVKNISTPIRVYRVLSFPGAAAHRVIKAKKALR
                     KTWRKTVLAIAAVLVIVVGVWAVWNYQFRAPPITEEVDPEKPVSTGIPEKPSIAVLPF
                     VNMSDDPKQDYFGDGLAEDMITDLAKISGLLVISRNSTFVYKDKSIGTKQIAENLGAR
                     YVLEGSVRKVGDQVRINAQLIDAPTQHHLWAERFDGPLSDIFALQDKIARKIVASLAV
                     KLTEQEQEHVYRKETDNIKAYDALLKGADLMKRYSPDRTVKAIPYFEEAIKLDPNYSR
                     AYAGLAEAYIFAGIYGGRLVGLSPQECFLRSEHNREISMKNPTYGSYIVNAYFFLPVQ
                     RRWEDAIADGERGVALAPKESSAWHALGWALIWGGRPEEAADVAGTALRVDQGCVY"
BASE COUNT          489 a          527 c          449 g          549 t
ORIGIN      
        1 tcaccaaatc aggaaacttg tgatatgcca aaaaaacaca aatatcttta gaccctaaaa
       61 agtacagata agttggagaa atcacagcat tttgggtggt gtcaaaacct ttggtaatag
      121 cagatacatt tactctaaga atcgatgtga tacgaaaggg ttatgtgtaa cgcttgggat
      181 aaccgaacag cctggtccca tcatccgtct tcaacggaga acggaacaag cccgtaaacg
      241 gcagtcccga tatattgatc ttttttctcc ttcgtgccct ctggattgat ataaacatgc
      301 ctgcaatctt tatagccccc ataaaggttt tcccactggt tgcaaagctt atcgccgtcg
      361 atccaacttt tgccggtatc tgcgatcttg tctctgcgaa ttgtagcctc cccgtcttcg
      421 gtacgttcaa ttgaccattc ttttccaccg cagatatcaa atccgctaat ggtccgccca
      481 aaaatctggt ctttgatctc ctttccagtc aggttgggct ccaggaatat tgcactctta
      541 taatacccgc caggttcccc cggcactcca gcctttagga gtccgtcagc ccaaagcttt
      601 tcagctttcg ggtctttgaa aggggccatc aaacattgca gattaagccc ctctttgata
      661 aaaggcgcta tgaactgtcg ggctttcttc gtacggccga ggagcgcatt ggtgaccgct
      721 aaccggctta agctcatgat tttattttcg ggctggaatt catatgctct ctcaaacgaa
      781 ttcagagctt cttccaagtt tcccatggcg aggtgaccca ccccgcgtgc attataagct
      841 cagtatacac atccttgatc tactctgaga gccgtaccag cgacatcagc agcctcctct
      901 ggtcttcccc cccaaattaa agcccaacct aaggcatgcc aagctgatga ttcttttggg
      961 gcaagagcaa caccgcgttc gccatctgct atggcatctt cccaccgccg ctgcacagga
     1021 aggaaaaaat atgcattgac gatgtaagaa ccgtatgtcg ggttcttcat ggagatttct
     1081 ctgttatgct cgctccgcag gaagcattct tgaggtgata agcccactag cctgcccccg
     1141 tatattcctg caaagatata tgcttctgcc agcccggcgt atgcccggct atagtttggg
     1201 tctaacttga tagcctcctc aaaataggga attgccttga ctgttctatc aggcgagtac
     1261 cgcttcatga gatctgcgcc ttttaagagg gcgtcgtagg ccttgatgtt gtccgtctct
     1321 tttcgataaa catgttcctg ctcctgctct gtcaatttca ctgccagcga agcgacgatc
     1381 tttcgggcga tcttgtcctg gagtgcaaaa atatcgctga gagggccatc aaaccgctcc
     1441 gcccagaggt gatgttgggt gggggcatcg atgagttgag cgttaatacg gacttgatcc
     1501 ccgaccttgc gcacactccc ttcaagcacg tatcgtgccc caagattctc ggctatttgt
     1561 ttagtcccta tggatttatc tttataaaca aatgtggagt tccgggagat gacgagcaga
     1621 cccgagattt tggccagatc agtgatcatg tcttcagcca gcccatcgcc aaaataatct
     1681 tgctttgggt catcactcat attgacaaat ggtaggacgg cgatggatgg tttttcgggg
     1741 attccggtgg atacaggttt ctcaggatcc acctcctcag tgatgggagg agcacgaaac
     1801 tgataattcc acacggccca gaccccaact acaataacca aaacagcagc tatggccaaa
     1861 accgtcttac gccaggtttt tcttaaagcc ttcttcgctt tgatcaccct gtgggcggct
     1921 gcaccaggaa aagacaatac ccgatagacc ctgatgggtg tgctgatgtt cttaacttga
     1981 tgctctccaa ggttttcgta ttctaatccg agtt
//
LOCUS       DBNGSX010000530         6890 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1646308, whole genome shotgun sequence.
ACCESSION   DBNGSX010000530 DBNGSX010000000
VERSION     DBNGSX010000530.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6890)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6890)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6890
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1646308"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="AC16CB_15690"
     CDS             <1..436
                     /locus_tag="AC16CB_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HIR3343965.1"
                     /translation="RFIELASEINTAMPRHVVDLVQDALNGQSKALKGSRVLVLGAAY
                     KPDIDDLRESPALDVIGLLEQKGALVSYNDPYIPSIRHDGVEMESVTDLMNAVKRSDC
                     VVIITNHSSYDYQAILETAQLIVDTRNALAGEGRDNPKVVRL"
     gene            453..1433
                     /locus_tag="AC16CB_15695"
     CDS             453..1433
                     /locus_tag="AC16CB_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3343966.1"
                     /translation="MANYLLTGAAGFIAYRVAEMLLEEGHRVVGIDNMNDAYDVRLKE
                     YRLMRLNSRPGFEFHQLDISDRDVFNQRSAISNQNFDGVINLAARAGVRQAVANPWVY
                     VDTNMTGTLNLLDFCQREGIRKFILASTSSVYGADAKLPTPEDDPSDRPLQPYAASKK
                     GAEALCHAYHHLYGIDVTVFRYFTVYGPAGRPDMSVFRFIQWISEGKALRLNGDGSQS
                     RGFTYVDDIARGTILGLKPLGFEVINLGGHESVKIIELIRMLEEIIGQKADIQHQPLN
                     PADMLANWANVEKAGRLLGWEPQVSLRQGIERTVAWYNAEREWARRISTD"
     gene            1586..2893
                     /locus_tag="AC16CB_15700"
     CDS             1586..2893
                     /locus_tag="AC16CB_15700"
                     /inference="COORDINATES: protein motif:HMM:NF024832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HIR3343967.1"
                     /translation="MTRRIIRNSGYLFSATGISAFISMLQSILAGRLLGVAGFGILGV
                     ITMFASVINRFASFRMGELVIKYVGYYTETGDRERAAAIYKAAALVEMLASLVAFGLI
                     WLLAPLGARYLAKDPNATGWFVIYGLIVLANLISESSTGLLQLFDRYRRMAALNVVQS
                     LFTLAIIALAYLTGGGLLHILLAYLGGKAIGALGLTLAALLEATRRWGTGWWRVSISL
                     LRPQVRELARFAVSTNISASLSLINKDSELLWVSFFRNPLETGYYKTALAIVNLAQLP
                     VSPLPQATYPELSRQAAHKDWAGMRHLLRQGSLLAGGFTLLVSLGLILLGKPLILLLY
                     KKPEFLPAYPALLILLAGFLVANTFYWNRSALLAIGLPDFPAKVNLVLAALKVIGVLL
                     LVPIYGYLASAALLAGSYLLGVTVSVIRFRAEIARQEQVGVAA"
     gene            2902..4053
                     /locus_tag="AC16CB_15705"
     CDS             2902..4053
                     /locus_tag="AC16CB_15705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3343968.1"
                     /translation="MLRVAYVLPALLRPSGWRTYAVALLRSIQEYVEPVIFASAADAE
                     EARSLFPQKQVFVLPVTQSAALTSLRGQLALLRCYRAISSNAFPKVDLVHSLEAYPTG
                     LVGSWLSRRLGCPHALTAHGTYGVVWHDLFLDRLAYRRVLRHASLVLSVSQGTETMLR
                     RYFGSDLAGARLRVILNGNDFTNRVPQQQALERSWPEVPTLLSVGDIKPRKGQHHSLA
                     AFAQLKKELPQARYWIVGKYQNNEYYLNMQRYVAENDLKDVSFLGTVSDDELDRLYRL
                     ASLFVLTPQQEGLSFEGFGLVYLEAGAYGLPVVATRSGGVADAVKDGHTGLLVEPGDV
                     EGIAVALLRLLTDRDLLRRMGRENRLWAESLTWERNAAEHYQAYREVCQ"
     gene            4050..5237
                     /locus_tag="AC16CB_15710"
     CDS             4050..5237
                     /locus_tag="AC16CB_15710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3343969.1"
                     /translation="MKIALVSPTHLPARRANTLQVMKMAQAMAGLGHVVRVAVPGFPS
                     EKVDWDDLAYQYGLQRQFPIEWLPVWPTMRRYDYGLRAVKWARGWGAELIYTRLPQAA
                     ALASILGMATIYEVHDFPPEGVATWLLRRFAQGRGARRLVCITRALAADLADRLGVPD
                     SPPFTVIAPDGVDLERYDDLPEPQRARQMLVGADHDYPLLQSYRFVAGYSGHLYAGRG
                     VELILDMAARLPEVGFLVVGGEPDESQALRSEVKSRRLENVIPVGFVPNAELPRYQAS
                     CDLFLMPYQRRVAASSGGDIARYLSPMKLFEYMACGRPILCSDLPVLREVLSPEIAVL
                     LPPDDVEAWVAAIQSLLNDPARCRSLGELVRAEAKGYTWEARASQILEGIERKFVFAE
                     HES"
     gene            5239..6738
                     /locus_tag="AC16CB_15715"
     CDS             5239..6738
                     /locus_tag="AC16CB_15715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HIR3343970.1"
                     /translation="MKRFSDVLYITLLALLLFGALLVIFDHAMLKGYFRDENQFIASG
                     KLLQDEGLLPYRDYPYFHMPNLTFIYALIYQVTDFTLLGARSFSVLCAWGTILALVYA
                     ANWFFQYRSRWLRLAVGAGGVLLLLPNPLFRFTVGLAWNHDFSVLLCLTAALVLLHVI
                     QFRNIKWLGLSGFLLGAAIGTRLTFMTALVPFLGILALFPNDRPSKNRARAWIYFGAG
                     FLVAMLPSILLFLSAPREFIFGNVQYAWLNTLYRQASEFEGPMTFSGKLLFLSERVLD
                     QPGTLALLAMVVLFPIFGAAENRRRSRSLILSAASLLKSTGTESTHNGLFYAFLFLLP
                     VFLLVGSFLPTPSWYQYFYAPLPFIVLGVLYSLSKLVRLSRLASYTGLMVFFLSVILV
                     NYFHFDKYPNLRSLARPGAWYPIQTRVLGYKIKTIVGEERVLTLSPLFPLDGGASIFP
                     QYATGPFAWRVADLLTKEQRFTYKVMSQDELIVTLDEKPAGGILVANYL"
BASE COUNT         1424 a         1723 c         1924 g         1819 t
ORIGIN      
        1 ccgctttatt gagctggcat ctgagatcaa cacagccatg ccgcgtcacg tagtcgactt
       61 agtgcaggat gcactcaatg ggcagagtaa agcattaaaa ggaagccgag tactggtact
      121 gggggcagcc tataaacccg atatcgacga cctgcgcgaa tcgcctgccc tggatgtaat
      181 tggattactg gagcaaaagg gtgctctggt ttcttataat gacccatata tcccctccat
      241 ccgccacgac ggcgtggaaa tggagagcgt gactgattta atgaatgccg tgaaacgttc
      301 ggactgcgtg gtgattatca ccaaccattc gagctacgat taccaggcta tcttggaaac
      361 cgctcaattg attgttgata cacgcaatgc gttagcaggt gaggggcgag acaatccaaa
      421 ggttgtgcgg ctttgaaaac gatggagtag cgatggcaaa ttatctgttg accggcgcgg
      481 ctgggtttat tgcttaccgg gtggccgaga tgctgctgga agaaggtcat cgcgtggtgg
      541 gcatagacaa catgaatgat gcctacgatg tgcgcctgaa agaataccgc ttaatgcggc
      601 tcaattcccg cccgggcttt gagtttcacc aactggatat ctctgaccgg gatgtcttca
      661 atcagcgatc agccattagc aatcagaatt ttgacggggt aataaacctg gcggcccgcg
      721 ctggcgtgcg ccaggcagta gcgaacccgt gggtgtacgt ggataccaat atgaccggca
      781 cgctcaacct gctggacttt tgccagaggg agggcatccg aaaatttatc ctggcctcaa
      841 cttccagcgt ctatggagcc gatgcaaagc tgcccacccc ggaggacgac cccagtgacc
      901 gcccattaca gccctacgct gccagcaaga agggtgccga ggcgctttgc catgcctacc
      961 atcatctgta tggtattgat gtgaccgtct tccgttactt taccgtgtac ggcccggcag
     1021 gacgcccgga tatgagcgtg ttccgcttca tccagtggat cagtgagggc aaagcgttgc
     1081 ggctaaacgg ggatggtagc caatcgcgcg gctttacgta tgtggacgat attgcacggg
     1141 gaaccatctt gggtctcaag ccgcttggtt ttgaagtcat caacctgggt ggtcatgagt
     1201 ccgtaaagat cattgaattg atccgcatgc tggaagagat cattggacag aaggcagaca
     1261 tccagcacca acctctaaac ccggcagata tgctggcaaa ctgggccaat gtggagaaag
     1321 ctggtcgctt gttgggatgg gagccgcaag tctcgttgcg ccagggtatt gagcggacag
     1381 tagcatggta caacgccgag cgggaatggg ctagacgaat cagtacggat tagccatttg
     1441 tagtgcatat ttgaattatc ggtcaacagg ttgatttaac ggatgagggt gtcaaacctc
     1501 atccaaactc catcctcttt attctgcgcc cattaatcat tcgatgattt tctccacctg
     1561 gtatagaagc ctcctgaata acccgttgac gcgccgtatc atccgtaact ctggctattt
     1621 gttcagcgca acgggtatct cagctttcat cagtatgctg cagagcatcc tggcggggcg
     1681 gttgttgggt gtggcgggtt tcggcatcct gggcgtgatc acgatgttcg ccagcgtgat
     1741 caatcggttc gcctcatttc gcatgggaga gctggtgatc aagtatgtgg gctactatac
     1801 tgagacaggt gaccgcgagc gcgcggcggc aatttataag gccgcggcgc tggttgagat
     1861 gctggcttcc ctggtcgctt ttggtttgat ctggctatta gcgcccttag gggcacgcta
     1921 tcttgctaaa gaccccaatg ctacggggtg gtttgtgatt tacgggctga tcgtgctggc
     1981 gaatctgatt tccgagagct ccaccggatt gctgcagctc ttcgatcgct accggcgtat
     2041 ggcagcctta aacgttgtcc agagcctgtt taccctggca atcatcgctc tggcatacct
     2101 cactggaggc ggtttgctgc acatcctact agcctattta ggcggcaagg cgatcggcgc
     2161 cctggggctg acgctggcag cgctcttgga ggctacgcgg cgctggggca caggttggtg
     2221 gcgcgtctct atcagcctat tgcgaccgca ggtgcgcgag ctggctcgct ttgccgtcag
     2281 caccaacatc agcgcctcgc tcagcctgat taacaaagac agcgagctgc tgtgggtctc
     2341 gttcttccgc aacccgttgg agaccggcta ttacaagact gcactggcga ttgttaacct
     2401 ggcgcaatta ccggtcagcc cgttgccaca agccacttat cctgagctgt cccgacaggc
     2461 agcccataaa gactgggctg gcatgcgcca tctcctgcgg cagggatcat tgctggcagg
     2521 aggctttaca ctgctcgtct ccctgggact tattttgctg ggtaagccat tgattctgtt
     2581 gctctataaa aagcccgagt tcctgccagc ttaccccgct ctgctcatcc tcctggcggg
     2641 attcttggtc gccaatacct tttattggaa ccgttcagcc cttctggcga ttggtctgcc
     2701 cgattttccc gccaaggtca acttggtatt ggctgcgctt aaggtgatcg gcgtgctcct
     2761 gcttgtcccc atttacgggt atctggcaag cgctgccctg ctggcaggct cctacctact
     2821 gggcgtcacc gtgtcagtga tcaggtttcg cgcagagata gcacgccagg agcaagttgg
     2881 tgtggctgcc tagaggttat cttgttgaga gtcgcttacg ttctgcctgc attgttgcgc
     2941 ccgtccgggt ggcgcaccta tgcggtcgcg cttttgcgat ctatacagga atatgttgag
     3001 ccagtgatct tcgcatcggc tgcggacgct gaagaagcgc gttctctttt tcctcagaag
     3061 caggtgttcg tgctcccagt gacccaaagc gcggccttga ccagcctgcg cgggcagctg
     3121 gccttgttgc gatgctatcg cgccatatcc agtaacgctt tccccaaagt tgatctagtg
     3181 cactctctgg aagcctatcc cacaggactt gtggggagct ggctttctcg ccgtttgggg
     3241 tgtccgcacg cgctaactgc tcatggaaca tatggggtgg tttggcacga cctcttcctg
     3301 gatcgcttgg cttaccgccg tgttctgcgg catgccagcc tggtgctctc ggtttctcaa
     3361 ggcacagaga ccatgctgcg ccgatatttt ggctccgacc tggctggcgc gcgcttgcgc
     3421 gtgattttga atggcaacga tttcaccaac cgtgtgcctc agcagcaggc gctagagcga
     3481 tcctggcctg aagtaccgac tttgctgtct gttggtgata taaagcctcg taaaggacaa
     3541 caccacagcc tggcagcctt cgcacagctt aagaaagaat taccacaggc gcgttattgg
     3601 attgtgggga aatatcaaaa taacgagtat tatctgaata tgcagcggta cgtcgctgag
     3661 aatgacctga aagatgtctc ttttttaggg actgtatcgg atgacgagct ggaccggctt
     3721 taccggctgg cctcgctgtt cgtgcttacc ccgcagcaag aaggtttgag ctttgagggc
     3781 tttgggctgg tttacctgga ggcaggcgcc tatggcttgc cggtcgtggc aacgcgcagc
     3841 ggcggcgttg cggatgctgt gaaagatgga catactggct tgctggtcga gccgggggat
     3901 gtggaaggga ttgctgtagc gctgttgcgc ctgctgactg atcgcgatct gctgcgccgc
     3961 atgggtcggg aaaaccgtct gtgggctgaa agtttaacct gggaacgcaa cgcagcagaa
     4021 cactatcaag cgtatcgaga agtatgtcaa tgaaaatcgc gcttgtttca cccacccacc
     4081 tgccggcgcg gcgcgctaat accctgcaag tgatgaagat ggctcaggcc atggctggct
     4141 tgggtcatgt ggtgcgggtg gcggtaccag gctttccctc agaaaaggtc gattgggacg
     4201 atctggcgta tcaatacgga ttacagcgcc agttcccaat cgaatggctg ccggtttggc
     4261 caacgatgcg ccgctatgat tatggtctgc gggcggtcaa gtgggcgcgt ggatgggggg
     4321 ctgaattgat ctatacacgg ctgccgcaag cggcggccct agccagcatc ttggggatgg
     4381 cgacaatcta tgaagtccac gattttcccc ctgaaggagt tgccacctgg ctgttacgcc
     4441 gcttcgctca aggtcgcggg gcacgccgac tagtttgcat tacccgcgcc ctggcggcag
     4501 acctggcgga caggttgggc gtacctgatt cgccaccttt cacggtgatc gcacccgatg
     4561 gagttgacct ggagcgctac gatgatctgc ccgaaccgca gcgagctcgg caaatgcttg
     4621 taggggcaga tcatgattat cccctgctgc aatcctatcg cttcgtcgct ggctatagcg
     4681 gtcatttgta tgctggacga ggcgtggaat taattctaga tatggctgcc cgattgcctg
     4741 aagtaggttt tcttgtggtg ggtggcgagc ctgacgagtc gcaggcgctg cgaagcgagg
     4801 taaaatctag gcggctggag aatgtcattc cagttggatt tgtgccgaat gctgagctgc
     4861 cgcgctacca ggcctcctgt gatctgttcc tgatgcccta ccagcgccgg gtggcagcat
     4921 cttctggcgg tgatattgca cgttatctga gtccaatgaa attgtttgag tacatggcct
     4981 gcggacgtcc aattttatgt agcgatctgc ctgtcctacg cgaggtgctt tccccagaga
     5041 ttgccgtgct attgcctcct gatgacgtcg aagcctgggt agccgcgatc caatctctac
     5101 tgaacgaccc agcccgctgc cgctcactgg gcgagctggt acgcgctgaa gcaaagggat
     5161 atacctggga ggcgcgcgct agtcagattt tagaggggat agaacgcaaa tttgtctttg
     5221 ctgaacatga gtcttagcat gaaacgattc agtgatgtgc tttatatcac gctgttagcc
     5281 ctattgttat ttggtgcgct attggtaatt tttgatcatg cgatgttgaa aggctacttc
     5341 agggatgaga atcaattcat agccagcggt aaattgctgc aagatgaggg tttgttgccc
     5401 tatcgggatt atccctactt tcatatgccc aacctgacgt ttatctatgc cttgatctac
     5461 caggtgacag atttcacact gctgggtgcg cgttcttttt cggttctctg cgcttgggga
     5521 acaatacttg ccttggtcta cgcagcaaac tggttttttc aatatcgctc caggtggttg
     5581 cggttagctg tgggggcggg aggtgtgttg ctactcctgc ctaatccgtt gttccgcttt
     5641 actgttgggc tagcctggaa ccacgatttt tcggtcttgc tttgcttaac agctgcactt
     5701 gtcttgcttc atgttatcca gtttcggaat attaaatggc tgggtctgtc tggtttcttg
     5761 ctgggggcag cgatagggac aagactaaca ttcatgactg ctttagttcc attcttggga
     5821 attttggcgc tctttcccaa tgatcgtccg tctaaaaata gagctcgtgc gtggatctat
     5881 tttggagctg gattcttggt cgccatgctg ccctccattt tattatttct ctctgctcct
     5941 cgggagttta tattcggaaa tgtccaatat gcctggttaa atacacttta ccggcaggct
     6001 tctgagtttg aaggccctat gaccttctct ggtaagctcc tttttttaag tgagagagtt
     6061 ttagatcagc caggcacact tgcattgcta gcaatggtgg tgttgtttcc cattttcggt
     6121 gcggcagaaa atcgaaggcg atcaaggagt cttatcctat ccgcggcttc tctactgaaa
     6181 tcaacaggga cagaatcaac acataatggc ttgttttatg cctttctttt cctgcttcct
     6241 gtttttctcc tagttggttc ctttttgcca accccttcct ggtatcagta tttttatgcc
     6301 cctttgccct tcatcgtttt gggagtcctt tacagtcttt caaagctagt tcgtctttcg
     6361 cgcttagcaa gttatactgg tctgatggta ttcttcctga gcgttattct ggtgaattat
     6421 ttccactttg acaaatatcc taatttgcgt tcactagctc gccctggagc ttggtatcct
     6481 atccagacta gagtcttagg atataaaata aagacgattg tgggtgaaga acgcgtcctt
     6541 acactttcac cactcttccc tttggatgga ggagcaagca tatttcctca atatgccacc
     6601 gggccttttg cctggcgcgt ggcagatctc ctaactaaag agcagaggtt cacctataaa
     6661 gtgatgtcgc aagatgagct gatcgtcacc ctagatgaaa agcctgctgg aggaatatta
     6721 gtagctaact atttatagtt taggtaatga taggtataag gtagagtatg ctggtggcac
     6781 taaagagtgg tggtttaaaa ataaagtaca ccgagacgga gcgcctgctg tagagagtgc
     6841 caatggtgat aaatactggt atcttaacga caaactacac cgtgaagatg
//
LOCUS       DBNGSX010000531         2340 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_815890, whole genome shotgun sequence.
ACCESSION   DBNGSX010000531 DBNGSX010000000
VERSION     DBNGSX010000531.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_815890"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="AC16CB_15720"
     CDS             complement(<1..376)
                     /locus_tag="AC16CB_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dimerization domain-containing protein"
                     /protein_id="HIR3343971.1"
                     /translation="MESQEIPKSEVIEQVYEWIYSLGALRAGLELRVWEKIAAGVDTA
                     EKMAAQEGWNLAGVRVLLDTICAFKLLTRDGERYCLVPESAYYLIPGKPTYKGGIVEH
                     EINWEGNGKLAEAIRSGKRPVVY"
     gene            complement(389..799)
                     /locus_tag="AC16CB_15725"
     CDS             complement(389..799)
                     /locus_tag="AC16CB_15725"
                     /inference="COORDINATES: protein motif:HMM:NF024865.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3343972.1"
                     /translation="MEPADDLRAFLRDWIDKSHYDLTRVEKCWSDQEGALCIFAAPHE
                     WLEGRQAILDYYRAALPGSGEYRVIVDHLDAYCEGSVGWVAARPVFVLPNGDQVPARF
                     TAVLHREENIWKFVQYHVSVGVTDEEFFHKAQPG"
     gene            complement(891..2141)
                     /locus_tag="AC16CB_15730"
     CDS             complement(891..2141)
                     /locus_tag="AC16CB_15730"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HIR3343973.1"
                     /translation="MKKVYCLFLSVLVAVIMGSCTNSPSASVKGIITPGKKIGEMTAE
                     QGTPTLIRPYIWQFCENMPDVHTPNASTIDCDVPMMSGMTIQLGWLAKESKFASNWDA
                     MTWELFIDGYKIDLDTFDWYDSDYPAHGEANKSRIWYINLKNLSPGEHTLRMLWESKL
                     AVDDGINVYQPGTYEHRVNFTVLEKAVYPVLPSTTIGQHPYTSEKAGLDFLLYLPKDY
                     GKNPQQKWPLIVYLHGAPVRGSTLELLMQEPLPQQLNTENDFPFIVVSPLGDGEFEFW
                     AKDTMINQLFTLLDEIKTEYSIDTKRIYLTGNDMGGNGVWVIGLLNPEYFAALVPLAG
                     YASYPFEVPENICELKNVPVWAFHGERDPYVPAQVAQDLVDALIACGGDAKITLSRQM
                     KMDVPFKVYKDPKLYEWMLSQIQE"
BASE COUNT          582 a          600 c          525 g          633 t
ORIGIN      
        1 cgtacaccac ggggcgcttc ccgctgcgaa tcgcctctgc cagcttgccg ttcccttccc
       61 aattaatttc atgttccacg atgccgcctt tgtaggtggg tttgccggga attaaataat
      121 aggcggactc cggtaccagg caatagcgct ccccatctct ggtcaacagc ttaaaagcac
      181 agatggtatc cagcaggacg cgtacaccgg ccaggttcca gccctcttgc gccgccattt
      241 tttctgcggt gtcgacgccc gcggcgatct tctcccatac cctgagttcc aacccggcgc
      301 gcagcgcgcc gagactgtag atccattcgt agacctgctc gatcacttcc gattttggta
      361 tttcctgcga ttccatattc cctccgatct atcccggttg agccttgtga aagaactcct
      421 cgtctgttac acccaccgag acgtggtact ggacgaattt ccagatgttc tcctccctgt
      481 gcagtacggc ggtgaaacgc gcaggaacct ggtccccatt gggaaggacg aagactggtc
      541 gagctgccac ccagccgaca ctgccttcgc aatatgcgtc caggtgatcc acaatcaccc
      601 ggtactcccc gctgcctggc agcgctgcgc ggtaataatc caggatcgcc tgacgcccct
      661 ccagccattc gtgtggggca gcaaagatgc acaatgcgcc ttcttggtca gaccagcatt
      721 tttccaccct ggtaaggtcg tagtgggatt tgtcgatcca atctcgcagg aaagccctta
      781 gatcatctgc tggttccatc tttcgtaact cttgctataa acgttcggct tacctcccga
      841 tactttcatt ggggacggca atagaaacgt gtaggtgaac catcccaata tcactcttgg
      901 atttgtgaca acatccactc ataaagtttg ggatctttgt aaactttgaa gggaacatcc
      961 attttcattt gtcggctgag agtaattttt gcatcccctc cacatgcaat tagagcatcc
     1021 accaaatcct gagctacctg agccggaaca tatgggtctc tttccccgtg gaatgcccag
     1081 acaggaacat tttttaattc gcagatgttt tcagggactt caaaagggta gcttgcatag
     1141 cctgcaagtg gcactaaggc cgcaaagtac tctggattaa gcaagccaat cacccaaaca
     1201 ccattaccgc ccatatcatt gccagtgaga tatattctct tggtgtctat agagtactca
     1261 gtcttaattt catccaataa agtgaatagc tgattgatca tcgtgtcttt agcccagaat
     1321 tcaaactccc catcccccag tggagatacg acgatgaagg ggaaatcatt ctctgtattt
     1381 agttgttggg gtagtggttc ctgcatgagt agttcaagag tggagccgcg tacaggcgca
     1441 ccgtgaaggt aaacgatcaa aggccatttc tgctgcgggt ttttcccata gtctttcggc
     1501 aggtagagca ggaaatcaag gcctgctttc tcagaggtat aagggtgctg accaattgtg
     1561 gtcgatggga gtaccgggta aacagctttt tctagtacag tgaaattcac cctgtgctca
     1621 tacgtaccag gttggtagac attaatgcca tcatcgaccg cgagtttcga ttcccataac
     1681 attcgaagag tgtgctcgcc aggagacaga ttttttagat ttatatacca aatgcgactt
     1741 ttattagcct ctccatgcgc aggataatca gaatcatacc agtcaaaagt gtctagatcg
     1801 atcttataac catctataaa taattcccat gtcatcgcat cccagttaga tgcaaatttc
     1861 gattctttgg cgagccagcc aagctgaata gtcattcctg acatcatcgg gacatcgcaa
     1921 tcgattgtag atgcgtttgg tgtgtgcacg tcaggcatat tttcacaaaa ctgccatata
     1981 tatgggcgta ttagggttgg tgtaccttgc tctgcggtca tttcaccgat ctttttccca
     2041 ggtgtaatta tccccttgac tgaggcagag ggactatttg tgcatgatcc cataatgacg
     2101 gcaaccagaa cagacaggaa caggcaataa acctttttca tgattgcctc ctttatatat
     2161 agagaacaga ttgagctgat gttaggtatg cttgttctgc caacgatagg caaaacttgt
     2221 gatttactcc acaaactcat tctactgcaa aaaaggataa aatcaagcca gtaaatgtga
     2281 actgcccaac tattggatat actgcatatt tgcagcatat cttaccaatt gggcgatttt
//
LOCUS       DBNGSX010000532         2148 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1301176, whole genome shotgun sequence.
ACCESSION   DBNGSX010000532 DBNGSX010000000
VERSION     DBNGSX010000532.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1301176"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="AC16CB_15735"
     CDS             <1..1010
                     /locus_tag="AC16CB_15735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3343974.1"
                     /translation="QQRGFRWLRWDLERRRTNPLTELPSLLAIATIYQRERPDLAHHH
                     TVKPVLYGSLATKMLGQPAIVNSITGRGYMYQSQSQGARLLRRLVEPLYRLAFSFPRG
                     AVIFENESDRQYFLKQGLISEDRTWLIPGVGVDPERFTPQPIPPEPVVILLAGRMLWD
                     KGVGVLVEAARLLRRRTTARVVLVGTPDPGNPATIDESILTQWNQEGVIEWWGWHADM
                     PSMYNQSHVVALPTAYGEGVPTVLLEAAACAKPLVASDIPGCRDVIQEGVNGILVPPN
                     DSQALADALEHLVRNPQLRSRMGAAGRQLVLEKFTYEQINTATLEVYQHVLNHDFTNR
                     N"
     gene            988..2019
                     /locus_tag="AC16CB_15740"
     CDS             988..2019
                     /locus_tag="AC16CB_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343975.1"
                     /translation="MTLPTATNDWTLRTEILEIAHRWPQVFIAFLLGSLLGWGAALIF
                     PTSYRAQTEIYVAYNADAIYRNPDDYKNWQFEELDAYIVSNDVLSETMQRLRQQDPYW
                     QDIKIKDVKPNLRTFWRSAGRWRLVADWSDQQHATQLSQTWTEVILETTSQAATHAQA
                     VLDLEAQIAAHSKDEVNVRLQFTQLTQLKNALETWRQAQDPASNQPLDTLARWRLQFL
                     AASIASQVPVDLALLAEFPAQGDPASAYFPAIDQAIIALDESVAVVERQLTDLTAQRE
                     ALLEQWKIESQASHNLTAHLTIEPISIENSSARPVRSTSQMALVGGSLGLLLWALVWL
                     ARPLYKAGR"
BASE COUNT          470 a          660 c          595 g          423 t
ORIGIN      
        1 tacagcagcg cggcttccgt tggctgcgct gggacctgga gcgacgacgc accaacccgc
       61 tgacagaact gccctcgcta ttggccattg ctaccatcta ccagcgcgag cgccccgacc
      121 ttgcccatca ccatacagtc aaaccggtgc tatatggctc tttggcgaca aagatgcttg
      181 gacagcccgc catcgtcaat tccatcaccg ggcgaggtta tatgtatcag tctcaaagtc
      241 aaggggcgcg cctgctccgt cgcctcgttg agccattata tcggttagcc ttttcctttc
      301 cgcgcggcgc ggtgatcttt gaaaacgaat ccgaccggca gtactttcta aagcaggggc
      361 ttatatcgga agaccgcacc tggctgatcc ccggcgtggg ggtggaccct gaacgcttca
      421 ccccccaacc catcccccca gagccagtgg tgatcttgct ggcaggtcgt atgctgtggg
      481 ataaaggagt tggcgtgctg gtggaggcgg ctcgtcttct gcgccgtcgc acaacagccc
      541 gcgtggtcct ggtgggcaca ccggatcctg gaaatccagc cacgatagac gaatccatcc
      601 ttactcaatg gaatcaagag ggagttattg aatggtgggg ctggcacgcg gacatgcctt
      661 cgatgtacaa tcagagtcac gtagtggcct tgccgaccgc ttacggcgaa ggcgtgccca
      721 cggtattatt agaggccgca gcttgtgcaa aacccctggt tgccagcgat attccaggct
      781 gccgggatgt catacaggaa ggcgtgaacg gaattctggt gcctcccaat gattctcagg
      841 cgttggcgga tgcgttggaa cacctggtac gcaacccgca gcttcgtagt agaatgggag
      901 ccgctggtcg tcagctagtt ttggaaaaat ttacctacga gcaaatcaac acagcgacac
      961 tagaagtgta tcaacacgtt ctcaatcatg actttaccaa ccgcaactaa cgactggacc
     1021 cttcgcactg agatacttga gatcgctcac cgctggccgc aggtgttcat tgcatttctc
     1081 ctgggcagcc tgctgggttg gggcgctgcg ttgatcttcc ccacctcata tcgcgcccag
     1141 acagagattt atgtagccta taacgctgac gctatctacc gcaaccccga cgattacaag
     1201 aattggcagt tcgaagagct ggacgcctat atcgtctcca acgatgtgct cagcgagacc
     1261 atgcagcggc tcaggcagca ggacccctac tggcaggaca tcaaaatcaa ggatgtgaag
     1321 cccaacctgc gcaccttctg gcgcagcgcc ggaagatggc ggttggtagc cgattggagc
     1381 gaccagcagc acgccaccca actatcccag acctggacag aggtcatcct ggagacgacc
     1441 agccaggcag ccacccacgc acaggcagtg ctcgatctgg aagctcaaat cgcggcccac
     1501 tccaaggatg aggtaaacgt caggctgcaa tttacccagc tcacccagtt gaagaacgcc
     1561 ttggaaacct ggcggcaggc gcaagaccct gccagcaacc agccgctaga taccttagcc
     1621 cgctggcggc tgcagttcct ggcagccagc atcgccagcc aggtgccggt cgatctggcc
     1681 ctgcttgcgg aattccctgc gcaaggcgat ccggccagcg cctatttccc cgccatagat
     1741 caggcgatca tcgccctgga tgagagcgta gcggtcgttg agcgacaatt aaccgacctc
     1801 acagcacagc gcgaagccct gctggaacaa tggaaaatcg aatctcaagc ctcgcacaac
     1861 ctgacggccc acctcacgat cgagccaatc tccatagaaa atagttccgc caggccagtg
     1921 cgcagcacca gccagatggc cctggttggc ggctcactcg gcttgctgct ctgggcgctc
     1981 gtctggctgg cgcggcccct gtacaaggca ggaagatgag cctggcatct tgggcaccgc
     2041 tcgccaataa gaccgtgggc atattgcaga agatcgcctg ggcgctgttc ctggtcttcc
     2101 tgccggtcac cagcttcccg ctctttccga aggtcatggg cggaagcg
//
LOCUS       DBNGSX010000533         3518 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_678518, whole genome shotgun sequence.
ACCESSION   DBNGSX010000533 DBNGSX010000000
VERSION     DBNGSX010000533.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_678518"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2661
                     /locus_tag="AC16CB_15745"
     CDS             <1..2661
                     /locus_tag="AC16CB_15745"
                     /inference="COORDINATES: protein motif:HMM:NF037660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3343976.1"
                     /translation="TKLYIPSLSPRVVHRPHLISRLNEGLARGCKLTLLSAPVGYGKT
                     SLISDWLSALNSRAAWLSLDTEDDDPLRFWVYVIAALQTIQPELGQTALTALQTAQMP
                     PIENMLIDLINQISTLSDKIILVLDDTHLIETTEIHQSLNFLLDHLPPQIHLVFITRE
                     DPPLPLPQMRAKGQISELRIKDLRFTMEESAEFLNHKMGLNLHPKGITALGQRTEGWA
                     AGLQMAALSLHELDDPAAFIEVFAGDNRYVADYLISEVLDRQPDHIRHFLLQTAIVDR
                     FTSSLCDALIGDTSHQSSRIIEKIEALGLFIMPLDHVRQWYRYHQLFADLLRYRLRQD
                     DPSRFMDLNRTASRWYEQQGLIDEAVKYGLVGEDHEYVAELIEISGLAMFGRNQFARL
                     QNWISALPEKIIQKHPYLSVLLVWVGSLTGQSDLARRHLALAEKNLSSAPADQHAEII
                     SHVALLRAYATRTSGDLDSSIKHAQEALSYLPENNVFLDCTIHLNLGGNYWLKGNFLA
                     VEGPLKHAISFIDIAEVEYPVLAAAGFLANAYLQQGKLHNAEALCKKIVERNSYHAHP
                     AAAYVLLELGELFFERNDLDGALEVLSKTIQIGEIADKVVNVIRARQLLARVYQALGN
                     QEEVANLMIQADELFKQTSPRYQVMHQIEYEYYRVRCLLFQQSTWGALQWADDYAKRR
                     EPVNNPWALLNELVYAHVLLIDGRPDQALSILKPCEDSARSYGARGWVVQNLALQSLC
                     CHATNDLEGALELLRSALSLAEPEGYVRTFVDYGLPMEQLLQLTAVRGIAADYVAKLL
                     AAFPAADVEKGSIELGKPSPHQPLIEPLTDREISVLRLMAANLSHREIADELYLSTNT
                     VKWHTTHIYGKLGVHRRAHAVSRAQELGIL"
     gene            2786..>3518
                     /locus_tag="AC16CB_15750"
     CDS             2786..>3518
                     /locus_tag="AC16CB_15750"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR3343977.1"
                     /translation="MKNFIEKHPFWFSLGFTIIVMQLLGFAVIVIGARILGLPELPIR
                     VAAQAATTIVPLILIWRLGWWEDIGLGSTTQNIYALAIPLTLTFFPLLLFGTTTMTSQ
                     RVSIFFLAVLFTGISEEIVYRGLFIRAFLPRGKWQAVLIPAVIFGFAHIVNSLGGGMS
                     LGDNLVQIANAFFYGLMFGAVRLRINNIWPLIVLHTLIDMFWVTAGLPDGVITLAEIP
                     LSEYLIEWVPSVVTAIYLMRKPIAVT"
BASE COUNT          854 a          877 c          854 g          933 t
ORIGIN      
        1 acgaaactct atatcccgtc actaagtccc agggttgtgc accgtccaca ccttatctcg
       61 cgtttgaacg aaggtttagc gaggggctgc aaactcaccc tgctatccgc gccagtggga
      121 tatggcaaga catctctaat cagtgattgg ctatctgccc taaacagccg agctgcctgg
      181 ctttcattgg acacggaaga tgacgaccct ctgcggtttt gggtatacgt gattgccgcg
      241 ctgcaaacca tccaacctga actcggccag acggcgctta ctgccctcca aacggcacaa
      301 atgcctccga tcgaaaatat gctcattgat ctgattaacc agatttctac gctctcagac
      361 aaaattatcc tggttctcga tgatacccat ctcattgaaa caaccgaaat acaccagagc
      421 ctcaattttt tacttgatca tttgccaccc caaatacatc tggtcttcat aacccgagaa
      481 gatcctccat taccgctgcc tcagatgcgt gcgaagggac aaattagcga gctgcgaatc
      541 aaggatttac gcttcacgat ggaggagagc gctgaatttt taaatcacaa aatgggactt
      601 aatttgcacc ccaaaggcat taccgccttg ggacaacgca cagaaggttg ggccgccgga
      661 ttgcaaatgg ctgcgctttc actgcacgag ttggatgatc ctgctgcatt cattgaagtt
      721 tttgcgggtg acaatcgtta tgtggccgat tacctgatct cagaagtatt ggatcgccaa
      781 ccagatcaca tccgccattt ccttctgcaa accgcaatcg ttgaccggtt cacatcatct
      841 ttgtgcgatg cattgatcgg ggacacatcc caccaaagca gcaggatcat cgaaaaaatc
      901 gaggcgctag gccttttcat catgcccctt gatcatgttc gccagtggta tcgctatcac
      961 cagttatttg cggacctact tcgttatcgt cttcgacaag acgatccatc cagattcatg
     1021 gatttaaatc gaaccgccag tcgctggtat gaacagcaag gcctaatcga tgaagcggtt
     1081 aagtatggat tggtcggtga agatcacgag tatgtagcgg aactgatcga gatctctggc
     1141 ttagccatgt ttggacgaaa ccagttcgct agactgcaaa attggatcag cgccctgcct
     1201 gaaaagatta ttcagaaaca tccgtattta tctgttcttc tggtctgggt cggctcattg
     1261 accggacaga gcgatcttgc caggagacat ctggccttgg ctgagaagaa tctttcttcg
     1321 gcacctgccg atcagcatgc tgaaattatt agccatgtcg ctctgcttcg ggcatatgcc
     1381 acgcgcacca gtggcgacct ggactcatcc atcaaacatg cacaggaagc cctgagttac
     1441 ctcccggaga ataatgtttt cttagactgt accattcacc taaacctggg aggtaactac
     1501 tggctcaaag gaaatttcct ggcggttgag gggccgctca aacatgccat ttctttcata
     1561 gatattgcag aggtcgaata tcctgttctt gcagcggctg gatttctcgc gaatgcctac
     1621 ctgcagcagg ggaaactgca taacgcggag gccttgtgta aaaaaattgt tgagcgcaat
     1681 agttatcatg cccacccggc tgctgcctat gtgttacttg agctaggcga gttgttcttc
     1741 gaaagaaatg atctcgacgg agctttggag gttctctcaa agacaattca gatcggagaa
     1801 atcgcggata aggttgttaa tgttattcgc gcccgccagc ttttagcacg agtctatcaa
     1861 gcgcttggca accaggaaga ggtggccaac ttgatgatac aggctgacga gctgtttaaa
     1921 caaaccagcc cccgctatca ggtcatgcat caaatagaat atgagtatta tcgcgtccgc
     1981 tgtctgctct ttcagcaaag tacgtggggt gctctacaat gggcagatga ttatgcaaaa
     2041 aggcgcgagc cggtcaacaa tccatgggct ttgttgaatg aactcgtcta cgctcacgtt
     2101 ttgcttattg atgggcgacc tgatcaggcg ctttcaatct taaaaccatg cgaggactct
     2161 gccagatcat atggtgcaag gggatgggtt gttcaaaatc tggcgcttca gtctctctgc
     2221 tgtcatgcga cgaatgattt ggaaggggca ttggaattgc tgcgcagtgc gctcagccta
     2281 gctgagccag agggctatgt ccgcaccttt gtcgattacg gattgccaat ggagcagttg
     2341 ttgcaactta ctgcggtgcg cggaatagca gcggattacg tcgctaagct cctggctgca
     2401 tttcctgcag cggatgttga aaaaggctcc atcgagctag ggaaaccttc tcctcatcaa
     2461 ccacttattg aaccgcttac agaccgcgag atttctgttc tgcggttgat ggctgcaaac
     2521 ctttcccacc gagagattgc tgatgagttg tatctttcga ccaataccgt aaaatggcat
     2581 acgactcata tctatggcaa attgggggta catcgaaggg cgcacgcagt atcgcgcgcc
     2641 caagaattgg gcattttata gcctttcgat aatcccatac caatcccata cctttgtatg
     2701 gtgacaaacc ataccttttt aggctacatt cagtgctaat gaatgtagtt ttttacattc
     2761 catacccata gaaggagaga agatcatgaa gaactttatt gaaaaacacc ccttttggtt
     2821 ctcgctgggc tttaccatca tcgtaatgca gttgctcgga tttgcggtca ttgtcatcgg
     2881 cgccagaata cttgggctgc ctgagcttcc gatccgggta gccgcgcagg cagcgaccac
     2941 aatcgtaccg ttgatcttga tctggcggtt aggttggtgg gaggatatcg gcttaggcag
     3001 caccacgcag aacatatacg cgcttgcgat tccactcacc cttacgttct tcccgctgtt
     3061 gctctttgga actaccacga tgacatcgca gcgggttagc atatttttcc tggcagtact
     3121 cttcaccggg ataagcgaag agattgtcta ccgcggcttg tttattcggg catttctacc
     3181 gcgtggtaag tggcaggcgg tcctcatccc ggctgtaatt ttcggctttg ctcacattgt
     3241 gaactctttg ggcggtggga tgtcgttggg ggacaacctg gtccaaatcg ccaatgcttt
     3301 tttctacggg ttgatgttcg gagccgtgcg cttgcgtatc aataatatct ggcctttgat
     3361 cgttctccat acactgatcg atatgttctg ggtaactgcg ggattacctg atggggttat
     3421 cacacttgct gaaataccct tgtcggaata tctcatcgaa tgggtaccga gtgttgtcac
     3481 cgccatatat ctcatgagaa aaccgatcgc agttacca
//
LOCUS       DBNGSX010000534         6530 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_747244, whole genome shotgun sequence.
ACCESSION   DBNGSX010000534 DBNGSX010000000
VERSION     DBNGSX010000534.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6530)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6530)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6530
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_747244"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="AC16CB_15755"
     CDS             complement(<1..727)
                     /locus_tag="AC16CB_15755"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HIR3343978.1"
                     /translation="MDGDDFLSGTNIAIIGLGLMGGSLAMALKGHCATLRGLDSDDMV
                     ISQALERKVVDTASSHPKAVLPGADIIILATPVGAIIDFIHILPEFHSGPAVVLDIGS
                     TKTEITQALDTLPPSFDPIGGHPMCGSEKSGLAYADPQIFRGATFAFTPLERTSQRAK
                     AVCTQITEVIGARALWLDSVTHDRWTAATSHIPYLLASALAATTSREAAPMVGPGFRS
                     TTRLAASSTRMMRDILATNRQNVL"
     gene            complement(733..1782)
                     /gene="aroF"
                     /locus_tag="AC16CB_15760"
     CDS             complement(733..1782)
                     /gene="aroF"
                     /locus_tag="AC16CB_15760"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR3343979.1"
                     /translation="MMIIMRSDATEEQIALVVERVETNDLRAHLSKGAERTVIGAIGD
                     GRPVNPDQFVFLPGVDRVLPISRPYKLASREFSQENTVFPLNGVSIGGDEIIVMAGPC
                     AVEDRSQLLETAIAVREAGAHVLRGGAYKPRTSPYSFQGLGEKGLELLAEARQITGLP
                     VVTEVMSPEQVPLVTSYADVLQIGARNMQNYALLHAAGASQYPVLLKRGMSATVEELL
                     MAAEYILSHGNRRVILCERGIRTFETATRNTTDINAIPVLKAMTHLPVILDPSHSTGY
                     WSYVTAIARAGVAAGADGLMVEVHTHPDQAFSDGGQSLKPERFAELVRQVRSVAQAVG
                     RTMETSKAYSNSVKA"
     gene            complement(1784..2173)
                     /gene="aroH"
                     /locus_tag="AC16CB_15765"
     CDS             complement(1784..2173)
                     /gene="aroH"
                     /locus_tag="AC16CB_15765"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013110283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HIR3343980.1"
                     /translation="MSVRGLRGATVAGEDRPEVILAVTRELLEAILEANPSLKLDDMA
                     SALFTVSADLRSVYPAQAARQMGWQLVPLMCAQEIPVPGSLERCIRVLIHWNTNLPPE
                     AVRHVYLHGAASLRPDLAGEQSSQRRQ"
     gene            complement(2428..3663)
                     /locus_tag="AC16CB_15770"
     CDS             complement(2428..3663)
                     /locus_tag="AC16CB_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013158958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase-like domain-containing protein"
                     /protein_id="HIR3343981.1"
                     /translation="MHTPWDHRYAQRTQRMGSSAIRELLKLTEKPDIISFGGGLPAPE
                     VFPVEEFHAACERVLRDHGAQALQYSTTEGYQPLREQIARHTSRYHIQINPENVMLTS
                     GSQQALDLLGKIFINPGDHILVEEPTYLGALQAWSAYGAEYVPVPADKCGMNTDYLEE
                     ALRAGPKFIYVLPNFQNPTGVTLSLERRQKLVELADRYGVPIVEDDPYGQLRYEGEHL
                     PAVVTLDDQFRNNKGARYHGNVIYLSTFSKILSPGLRLGWVVAPEEVIRKLVQAKQGA
                     DLHTATFNQLVAYEVSRGGFLDQHIKVIRDVYKERLSVMLSAMDRFFPAEVDWTQPSG
                     GLFLWGIMPEYLNSEDVLRDALEKKVAFVPGESFFPRGGGQNTMRINFSNATPEKIQQ
                     GISRLGEVLLEKIRQKATA"
     gene            complement(3994..>6530)
                     /locus_tag="AC16CB_15775"
     CDS             complement(3994..>6530)
                     /locus_tag="AC16CB_15775"
                     /inference="COORDINATES: protein motif:HMM:NF013720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3343982.1"
                     /translation="IAGYSSYPWAVDWARFKEIAKSVGAYLFADIAHVAGLVAAGAYP
                     SPLGFADVITFTTHKSLYGPRGACILTTEPSLARKIDRAVFPGEQGGPHVNVFAAMAL
                     AFKLAKTEQFKQLQAQVVNNCIKLTKRMEERGLRIPFGGTNTHLTNVDCSTIKSPDGT
                     TLSGDQTARILDIAGIVVNRNTIPGDPSAAAPSGIRLGTPWVTQRGLDEADMVALADI
                     IADLMLAMTPFGLGGRKGEARRAKMDFTVLEEAKIKVRSLAEKAGIDFDPIQHGYPHF
                     YYLDDKPVSDGWVAFDLVGERVRQFLDYAASCDVDEIKPGESAATCIFTPTGTVEGAL
                     TRDLDGAFRLSVPSWQGGLAAAWLRDLSDGYVGFDDDLSRKLPGPVVVRQSSAAPVEK
                     SCLEEPSLSPSRGTGKPYFIGMPAGTGQALPKFEWQEQEAALRRTPIYEVHKKLGAKL
                     IPFAGWEMPVWYTSVVEEHLATRQAAGLFDVAHMGVYQVEGPDAAVFLDSVCGNDISG
                     LGVGESCYTHFLTPDADVIDDTLVYHRAVDKFLVVVNASNDDKDWAWLNAVKNGSVCV
                     DNSRPWTKAYGRGAVLRNLRDPKEGADMRVDIALQGPKSRDVLLAMGCDAETRRRILG
                     LKRTQLCDAVVGGFDLVVSRTGYTGEKMAFELFVHPSRAAELFTALLQVGEIVGLKPV
                     GLGARDSLRTEAGLPLYGHEMGLGSGKAGDPDLGVAEGGFGSYVKTYKPWFIGRSAYL
                     EREKSRKAVVVRFRFTEKGVRMAHGGDPVLDKRGRVIGWVTSCAVDSDGLLTGQAYLE
                     LKSTEEGTLIYIYQSAPDKPGKAPAELQPGDRATLPTPAVVIKRFP"
BASE COUNT         1545 a         1897 c         1603 g         1485 t
ORIGIN      
        1 ccagcacatt ttggcggttg gttgcgagga tatcccgcat catgcgcgtg gacgaggcag
       61 ccaggcgcgt ggtgctgcga aagcctggac caaccatggg ggctgcttcg cgtgaggtcg
      121 tcgcggccaa ggcgctggct aacaggtatg ggatatggct ggtagcagcc gtccagcggt
      181 catgcgtgac tgagtctaac cagagcgctc gagctccgat cacttcagtt atctgtgtgc
      241 atactgcctt cgccctctgg ctggttcgtt ccaagggtgt gaacgcgaag gttgcacctc
      301 ggaatatttg cggatcagcg tacgccaaac cagatttttc gctaccgcac atcgggtgcc
      361 cgccaattgg gtcaaaactt ggcggcaagg tgtctaaggc ttgggtgatc tcggttttgg
      421 tagagccaat gtccagtaca accgccggcc cggaatgaaa ctctggcaaa atatgaataa
      481 aatcgataat tgcaccaacc ggtgttgcca gtatgataat atccgcgccg ggaagcactg
      541 ctttagggtg ggaagatgct gtgtccacca ctttgcgctc caatgcctgc gagatgacca
      601 tgtcatcaga atccaggcct ctaagggtcg cgcaatgtcc cttaagcgcc atggccagcg
      661 acccacccat caagcctaac ccaataatag caatgttcgt tcctgagaga aagtcatcac
      721 cgtccattac gatcaagcct tgaccgagtt ggagtaagct ttcgacgttt ccatggtacg
      781 acccactgct tgtgccacag atcgcacctg gcgaaccaat tcggcaaaac gctcaggctt
      841 cagcgattgc ccgccatctg agaaagcttg atcaggatga gtgtggactt ccaccatcag
      901 tccatccgcg ccagcagcca ctcccgcgcg cgcgatggca gtcacgtatg accaataacc
      961 agtgctgtga cttgggtcaa gaatgacagg caggtgggtc atcgccttga gcaccggaat
     1021 ggcgttaatg tcggttgtgt tgcgtgtggc ggtctcgaaa gtccggatgc cgcgctcgca
     1081 caggattact cgccggttgc cgtgcgagag gatgtattcg gctgccatca acagctcttc
     1141 caccgtggcg gacatgccgc gcttcagcaa aacaggatac tggctggcac cagctgcgtg
     1201 caacagcgca taattttgca tgttacgcgc gccgatttgt aacacgtcag catagcttgt
     1261 cactaatggc acctgttctg gtgacataac ctcggtcact accggcaacc cagtgatctg
     1321 gcgcgcctcc gccaacaact ccaatccctt ttctcccaaa ccctgaaagg aataggggga
     1381 tgtgcgcggt ttatatgccc caccgcgcag gacgtgagcg ccagcttctc gaaccgcaat
     1441 ggcagtctcc aataactgcg agcggtcttc tactgcgcac ggccctgcca tgacgatgat
     1501 ttcatcgcca ccaatggata ccccatttag tggaaatacc gtgttttcct ggctgaactc
     1561 cctggaggcc agcttataag gccgggagat cggcagcacc cggtcaacac caggtaaaaa
     1621 cacgaattga tccggattaa ccggcctccc atcaccgatg gcgccgatga cagttcgctc
     1681 ggcacctttg gacaggtggg cgcgcaggtc gttcgtctct actcgctcaa ccaccagcgc
     1741 tatttgttcc tcggtcgcgt cagaacgcat gatgatcatc attctattgc ctcctttgag
     1801 aagattgttc acctgctaaa tcagggcgaa gacttgccgc tccgtgtaga tacacatgcc
     1861 tgaccgcctc gggcggcagg ttggtattcc agtgaatcag cacccgaata cagcgctcca
     1921 aactgcccgg gacgggaatc tcttgggcgc acatcaacgg gaccagttgc cagcccatct
     1981 gtcgggctgc ttgggccggg taaacactgc gtaaatctgc agagacggtg aaaagggcgc
     2041 tggccatatc gtccagcttg agagagggat tggcttccag gattgcctcc agcagctccc
     2101 ttgtcacagc caggataact tcgggtcgat cttctcctgc cacagttgcg ccacgcaaac
     2161 ctcgtacaga catgtacaca tcctccagat aatcaaaaac gcgagaccgt tggtctcgcg
     2221 ttttgggttt gaaaatgtac tctaccctgc taagcgccgc caacggcaac ctgtgccagg
     2281 aagccgtaaa aataaaagcg cacaaaaaac agggtcgagg agaaagtcat cgcaattttc
     2341 gatttgggat aggatttatc accaattgct ccaagtttat ctaaagaatg cataatcgtc
     2401 aagaagcaat ttttaaaccg acacctttca agccgttgct ttttgtctaa tcttctcgag
     2461 taaaacctct cccaggcgag aaattccctg ctgtattttc tccggtgtag cattcgagaa
     2521 gttgatgcgc atggtgtttt gaccgccgcc tcttgggaaa aaagattcgc cgggcacaaa
     2581 cgccaccttc ttctctaatg catcccgcaa cacatcttct gagttcaaat actctggcat
     2641 gatgccccat aaaaacaggc caccagaagg ctgcgtccaa tccacttctg ccggaaagaa
     2701 acgatccatg gctgacagca ttacactcag ccgctctttg tacacatcgc ggatcacctt
     2761 gatgtgctga tccaggaaac cgccacgcga tacctcgtaa gcgacaagct gattgaaggt
     2821 ggcagtatgc aaatctgccc cttgctttgc ctgcacaagc ttgcgaatca cctcctccgg
     2881 ggcgactacc caacccaagc gcaacccggg cgataatatc ttagagaatg tgctcagata
     2941 gattacatta ccgtgatagc gtgcaccctt gttattccga aattggtcat ccagtgtcac
     3001 cacagcaggc aggtgctctc cctcgtaacg caactggccg taagggtcat cttcgacgat
     3061 gggtacgcca tagcggtcag ccagctcaac cagcttttgc cgccgctcta acgagagggt
     3121 gacgccagtc gggttttgga aattcggcag aacataaata aattttggtc ccgcgcgcag
     3181 ggcttcctca agataatctg tgttcatccc gcacttatct gctggcaccg ggacatactc
     3241 cgccccgtaa gcactccaag cctgtaaggc acctaaataa gtaggttctt caaccagtat
     3301 atgatcaccc ggattgatga aaatctttcc caacagatcc aaagcttgtt gtgatcccga
     3361 tgtaagcatt acattctctg ggttaatttg tatatggtaa cgggatgtat ggcgggcgat
     3421 ctgctctctt aatggctgat aaccttctgt tgtgctgtac tgcaatgctt gtgcgccatg
     3481 atccctcaaa acccgttcac aggcagcgtg aaattcttct actgggaaga cttctggagc
     3541 aggtaaaccc ccaccaaatg aaataatatc cggtttttca gttagtttca acagctcgcg
     3601 aattgcggag ctgcccatcc tttgggttcg ctgtgcatag cgatgatccc atggagtatg
     3661 catagagtgt ttctccttta tataacaaat ctcccgaagt ttgggtaaac ttcgggagtt
     3721 acatacgatc tgtccatcac ctgaacaaaa agcatcaggc caaaaaaagc tatgagcata
     3781 ccaagtacca taaacatgtg atgcattttt attactttga accgatattg tacgttcatg
     3841 gcaattatcg gttcacccct agtatttcta ttataaccga atgataccct cctaacccta
     3901 tcaaccttgc gaggcaggga gattgcatat gccatcacgg gcatatgcaa tctcagaaat
     3961 cgaggggggt gtgtctccct agcttctcct tggctatgga aatcgtttga tcacaacagc
     4021 tggggttggc agagtagctc tgtctcctgg ttgtaactca gcaggcgctt tcccgggctt
     4081 gtcgggggca ctttgataaa tatagatcag cgtgccttct tctgtgctct taagctcaag
     4141 atacgcttgc ccggtaagca gtccatcgct atcgacagca cagctcgtta cccagccaat
     4201 cacacggccg cgcttatcca gtaccggatc accaccatgc gccattcgca cacctttctc
     4261 agtgaagcga aaacgcacca ccacagcttt gcggctcttc tcacgttcga gataagcact
     4321 cctcccaatg aaccagggtt tgtacgtttt gacataactg ccaaatccgc cctctgctac
     4381 acccagatca ggatcccctg cttttccaga gccaaggccc atctcatgcc cgtagagtgg
     4441 caacccagcc tcagtgcgta acgagtcgcg cgcccccaag cccactggct tgagcccaac
     4501 tatctcgcca acctggagca acgcagtgaa caattctgcc gcccggctag ggtgcacgaa
     4561 caactcaaat gccattttct ctcccgtata gccggtgcgt gaaaccacca ggtcaaaacc
     4621 ccctactacc gcatcgcata gttgggtgcg ttttagcccc aagatacgcc ggcgtgtttc
     4681 cgcatcacaa cccatagcca gtagcacatc gcgggacttc ggtccttgca atgcaatgtc
     4741 tacacgcatg tccgcacctt cttttgggtc tcgcaggttg cgcaaaacgg ctccccgacc
     4801 ataagctttc gtccacgggc gggaattatc cacacaaacg ctgccattct tcaccgcgtt
     4861 gagccaggcc cagtccttgt catcgttgga agcgttgacc accaccagga atttatccac
     4921 tgcacgatgg tataccagcg tatcatcgat tacatcagcg tcgggggtga gaaaatgcgt
     4981 ataacaggac tcaccaaccc ctaacccgct gatatcgtta ccacacacgc tatctaaaaa
     5041 gaccgctgca tctggacctt ccacctggta gacccccatg tgcgctacat cgaacaaacc
     5101 ggcggcttgc ctcgtcgcca ggtgctcctc aacgaccgag gtgtaccaca cgggcatttc
     5161 ccagcctgca aaggggatta atttggcacc tagctttttg tgcacctcat agattggtgt
     5221 gcgacgtaag gcagcctcct gttcttgcca ctcgaatttg ggcagcgctt gccctgtccc
     5281 ggctggcatc ccaatgaaat agggtttgcc tgtaccgcgc gaaggagaga ggctaggctc
     5341 ctccaaacac gatttctcta caggcgctgc actagattgc cgcacaacca ccgggccggg
     5401 cagtttacgc gatagatcat catcaaatcc cacataccca tccgacaaat ctctcagcca
     5461 cgcagccgcc aacccccctt gccaggaggg tacactcaga cggaacgcac cgtccaagtc
     5521 gcgggtgaga gctccttcaa cggtccccgt gggtgtaaat atgcaagtcg cagcgctctc
     5581 tcctggctta atttcatcca catcacaaga tgctgcataa tccaaaaact gccgtacgcg
     5641 ctccccaacc aaatcgaacg ctacccaacc atcggagacg ggtttgtcat ccaggtagta
     5701 gaagtgcggg taaccatgct ggattggatc aaagtcgatg ccggccttct ccgccaaact
     5761 gcgcaccttg attttagctt cttcaaggac agtgaaatcc atcttggctc gacgggcttc
     5821 gcccttgcgt ccccccagcc caaagggcgt catcgccaac atcaggtcgg cgatgatgtc
     5881 cgccaatgcg accatatcag cttcatcgag tccgcgctgg gtcacccaag gcgtacccag
     5941 acggatgcct gacggagccg cagccgatgg atcacctgga atcgtattgc ggttcaccac
     6001 aatacccgcg atatccagga tccgcgcggt ttgatcgccg gagagagtcg tcccatcagg
     6061 gcttttgatg gtgctgcaat ccacattggt caggtgggtg ttcgtcccac caaatgggat
     6121 gcgcagaccg cgctcctcca tccgtttggt gagcttgatg cagttattga ccacctgcgc
     6181 ttgcaattgc ttgaattgct cagttttcgc cagcttgaat gccagagcca tagcggcgaa
     6241 tacattaaca tgcggaccac cttgttcacc aggaaagaca gcccggtcaa tcttgcgtgc
     6301 caaggaaggc tcagtggtca agatgcaagc tccccgtggg ccgtatagcg atttgtgcgt
     6361 ggtgaaggtg atcacatccg caaagccaag tggggaggga tacgcacctg ccgccaccag
     6421 accagccaca tgggcgatgt cggcaaacag gtacgcgcca accgatttgg cgatctcttt
     6481 aaagcgagcc caatcgaccg cccagggata cgacgagtac cctgcgataa
//
LOCUS       DBNGSX010000535         8830 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1647052, whole genome shotgun sequence.
ACCESSION   DBNGSX010000535 DBNGSX010000000
VERSION     DBNGSX010000535.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8830)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8830)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8830
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1647052"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..1636)
                     /locus_tag="AC16CB_15780"
     CDS             complement(200..1636)
                     /locus_tag="AC16CB_15780"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3343983.1"
                     /translation="MSLRTRLAVLYTSIVGGILFLFGTAVYGAVSFSLISQVDGLIQN
                     TVEQIWPNIYVDSQGVLGVRNSPDLELAPGLLFQIWGRQEQLIYTNTPQIITPLDSIG
                     IEYSEPVFRYSTLNSTIGSVHLRVITVPMVIGAGGRKAGSLQVASSLAVIDTTQRALL
                     WVLVVGSVSAMGIAGIAGWMSTRQALAPLEGVTQVATQITKADDLSRRIPYQGPSDDE
                     VGQLIQAFNQTLGRLENLFNTQRRFLADVGHELRTPLTVIRGNVDLMRRMGGIDTESM
                     DSIENEVDRLTRLVGDLLLLAQAESGKLPLNIRLVELDTLLLDVMQQMRVLTHNRLNL
                     KLGEIDQVLVCGDQDRLKQVFVNLIGNAIQYTPEGGDVVVSIGKVGERGRVVITDTGQ
                     GIPQEDLPHIFERFYRGSKSRTRQKDGKGYGLGLSIAYWIIRNHRGQIDVDSQPGMGT
                     SFYVWLPLAHDDCVTSTTEAAQGDTNNK"
     gene            complement(1643..2320)
                     /locus_tag="AC16CB_15785"
     CDS             complement(1643..2320)
                     /locus_tag="AC16CB_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006102617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3343984.1"
                     /translation="MSSRILVIEDDEAILKFLRRGLAYEGYQVDTAADGTIGLNIARD
                     NPPDLVVLDLMLPGIDGLEICRRLRAGGPVPILILTAKDTVNDRIMGLDMGADDYMVK
                     PFNLDELLARIRALLRRSQPSRPQVLRFADLSLDTGTRQASRGERIISLTAKEYELLE
                     LFLRHPRQVLTRDMIFDRVWGYDFGGESNIIEVYIRYLRQKLEEINEPRLIHTVRGMG
                     YVLRESN"
     gene            complement(2436..3110)
                     /gene="rpe"
                     /locus_tag="AC16CB_15790"
     CDS             complement(2436..3110)
                     /gene="rpe"
                     /locus_tag="AC16CB_15790"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015891988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR3343985.1"
                     /translation="MHVKIAASILAADFARLGNQVQEALDGGAEYIHVDVMDGHFVPN
                     LTIGPMIVSAIKPIVQQANAILDVHLMVEEPDRLVPLYVQAGADNITVHVEASRHLHR
                     TIQHIKEMGVKAGVTLNPATSLSTLDEIISAVDIVLVMSVNPGFGGQSYISSSTDKIT
                     RLRHMLDARGLSHVDVEVDGGIRPSNVPEIVRAGANVLVCGTSVFNSAASIEENIAAL
                     RAAIPG"
     gene            complement(3156..3875)
                     /gene="rpiA"
                     /locus_tag="AC16CB_15795"
     CDS             complement(3156..3875)
                     /gene="rpiA"
                     /locus_tag="AC16CB_15795"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HIR3343986.1"
                     /translation="MAKHEIQNSKYKAARRALDYVRDGMTLGLGSGSTAAHFVDLLGE
                     QIRSGSLREIRGVPTSEATANQARALSIPIISLSSSNPGGEPRLDLAIDGADEVDPSL
                     NLIKGLGRALLREKVVEIHAERLIIMVDETKMVPRLGTRGPLPVEIIPFEAETHVRWL
                     NTLGCRAELWVEEDGSPVITDNNNYLVRCWFERGISDVYSLARRLAERPGIVEHGLFL
                     DMATLVIIAGEQSTRIIERST"
     gene            complement(3963..4376)
                     /locus_tag="AC16CB_15800"
     CDS             complement(3963..4376)
                     /locus_tag="AC16CB_15800"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3343987.1"
                     /translation="MPKRTLTLVIAIVVLSTLLVACGGTDTADGGSGGDAKRGEELYK
                     QTVIGSQAAPGCITCHSLEAGKVLIGPPHAGLATKAETAVPGMPAEDYLRESIIEPDA
                     HLVDGFSAGIMYQNYAKDLKESEINDLLAFLLTLK"
     gene            complement(4611..5000)
                     /gene="rplL"
                     /locus_tag="AC16CB_15805"
     CDS             complement(4611..5000)
                     /gene="rplL"
                     /locus_tag="AC16CB_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003523726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIR3343988.1"
                     /translation="MANIDKLVEELSKLSVLEASQLVQKLEEAWGVSAAAPVAMAAMA
                     SPAAAGAPAEAEEEKTEFDVIIKDAGPKKIEVIKTIRQLSSSLGLKEAKELAETVGGK
                     VMEAVSKDAAMDAKSKLEAAGAVIEVK"
     gene            complement(5104..5640)
                     /gene="rplJ"
                     /locus_tag="AC16CB_15810"
     CDS             complement(5104..5640)
                     /gene="rplJ"
                     /locus_tag="AC16CB_15810"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIR3343989.1"
                     /translation="MAITKKRKHEVVAQYSEWANRCQALILTEYTGITMKQLDELRKK
                     AREAGGEFHIVKNTLASLALKDQDMPLPEGFFEGSTAVGFAFTDGPALAKAISEFTRT
                     SEFIKIKGGYLGKQAMTAEDVKALAELPPLPVVRAQLLGTLLAPASKLARTLAEPARM
                     VAAVVKAYADRESAPEAA"
     gene            complement(5892..6611)
                     /gene="rplA"
                     /locus_tag="AC16CB_15815"
     CDS             complement(5892..6611)
                     /gene="rplA"
                     /locus_tag="AC16CB_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIR3343990.1"
                     /translation="MTKHGKKYLAAAAKVDKEQLYTPAEAVALAKETSISKFDGTVEI
                     HMRLGVDPRHADQQVRDVVVLPHGLGKTVRVLVFAQGEGASLAREAGADLVADDDETL
                     AKIQAGWTDFDVAIATPDMMGKVGRLGRVLGPRGLMPNPKAGTVVQAEDIPRVVNESK
                     AGRVEFRLDKTANLHVPIGKVSFEQNKLYENLAALMEAVKKARPAASKGTYIKRITLT
                     TTMGPGIKVDPNQAQAMEVLE"
     gene            complement(6662..7087)
                     /gene="rplK"
                     /locus_tag="AC16CB_15820"
     CDS             complement(6662..7087)
                     /gene="rplK"
                     /locus_tag="AC16CB_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011056150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIR3343991.1"
                     /translation="MAKKLKTIIRLQIQAGKANPAPPIGPALAGHGINLMAFCKEYNA
                     RTSSRMGEIIPAEISIYTDGSFTFILKTSPAAVLLRKAAGVDKGSAQPNREKVGKVTR
                     AQVREIAELKMKDLNALDIEGAMRQIEGTARNMGIQIID"
     gene            complement(7197..7868)
                     /gene="nusG"
                     /locus_tag="AC16CB_15825"
     CDS             complement(7197..7868)
                     /gene="nusG"
                     /locus_tag="AC16CB_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIR3343992.1"
                     /translation="MEEKVPEEQPLMTEDEITEVETPQDELSAESLDSEESLDVPVEI
                     EDQAEDDRAWYVVHCYSGYENKVRHNLEQRIETMGMKDKIFDIVVPTEEEIEVKEGKR
                     RTVERRVFPGYILVNMILSEESWYVVRNTPGVTGFVGMGNNPTPLRPEEVAQIIRRME
                     AEAPRIKVTFKSGERVRIVDGPFNDFRGTVSEIDMERAKVRVMVNFFGRETPVELDFL
                     QVEKA"
     gene            complement(7932..8156)
                     /gene="secE"
                     /locus_tag="AC16CB_15830"
     CDS             complement(7932..8156)
                     /gene="secE"
                     /locus_tag="AC16CB_15830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HIR3343993.1"
                     /translation="MAKADKGKSIERRSSNKIQLFFRETVGELRKVSWPTWQDAWNLT
                     KIVLVVMVVMGLYLGVLDYIFTTLMALILG"
     gene            complement(8266..8341)
                     /locus_tag="AC16CB_15835"
     tRNA            complement(8266..8341)
                     /locus_tag="AC16CB_15835"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8306..8308),aa:Trp,seq:cca)
     gene            complement(8402..8569)
                     /gene="rpmG"
                     /locus_tag="AC16CB_15840"
     CDS             complement(8402..8569)
                     /gene="rpmG"
                     /locus_tag="AC16CB_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR3343994.1"
                     /translation="MASKKDIRPVITLACTECKERTYTTEKNRRNDPGRMELNKFCPR
                     CRKHTLHRETK"
     gene            complement(8580..>8830)
                     /locus_tag="AC16CB_15845"
     CDS             complement(8580..>8830)
                     /locus_tag="AC16CB_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu C-terminal domain-related protein"
                     /protein_id="HIR3343995.1"
                     /translation="RKEEGGRHKAFFTGYRPQFYIRTMDVTGNITLPEGVEMVMPGDN
                     VNLEVELIVPVALEQGSKFAIREGGLTVGAGVITKILD"
BASE COUNT         2009 a         2448 c         2160 g         2213 t
ORIGIN      
        1 aatgcgcata tatctgcgaa atcagctcaa gctttcaacc cagcaccaaa accaagtcca
       61 ggagatgttg tctactctcg ccctgaacca acctaccatc cagtctcaat gcaggctaga
      121 tcagcaagaa atgaatcaac tattattaga cgctaaaaac acaaacaaaa tgaccctgaa
      181 gatgtgtatt tcttcagggt catttattgt ttgtatcacc ctgcgccgcc tcggttgtgc
      241 tagtcacaca atcatcatgt gctagaggca accatacata aaaagaagtg cccatcccag
      301 gttgagaatc gacatcaatc tgacccctat ggttgcgaat gatccagtaa gctatcgaga
      361 gacctaatcc atatcctttg ccatcttttt gacgcgtgcg cgactttgat ccacggtaga
      421 agcgttcaaa tatatggggg agatcttctt gtggtattcc ttgacctgtg tctgtaatga
      481 ccacccgtcc tcgctcacct actttgccaa tgctcaccac cacgtcccca ccctcgggtg
      541 tatattgtat ggcgtttcca atcaaattca caaacacctg ttttaaccga tcctgatccc
      601 cacacacaag cacctgatcg atttcaccaa gctttaaatt tagacgatta tgcgtcaaaa
      661 ctcgcatttg ctgcataaca tccaacaaca gcgtgtctaa ctcaaccaat cgaatgttca
      721 gggggagttt accggattca gcctgtgcga ggagcagcaa atctcccacc aggcgggtca
      781 aacgatccac ctcattctca atgctgtcca tcgattctgt atctatgcca cccatcctgc
      841 gcatcaagtc cacatttccg cgaatgacgg ttaagggtgt gcgcagttca tgcccgacgt
      901 cagccagaaa gcgcctctgc gtattgaata agttctccaa gcgtcccaat gtttggttga
      961 aagcctggat gagttgccct acctcatcat ctgatggacc ttggtaggga atccgcctcg
     1021 aaaggtcatc ggctttggtg atctgtgtcg ccacctgtgt gacgccttcc aacggggcta
     1081 aagcctggcg agtgctcatc catcctgcga ttccagcgat tcccatcgct gaaacgctac
     1141 ccacaactag cacccacaaa agcgcacgct gtgtggtatc gatcaccgct agactggatg
     1201 ccacttgcaa ggatcctgcc ttacgccctc ccgcaccgat caccattggg actgtaatca
     1261 ccctaagatg tacagatcca attgtgctgt tcaatgtaga atagcgaaat accggttcgc
     1321 tatactctat cccgatacta tccaaaggcg taataatttg gggagtattg gtgtatatca
     1381 gctgttcctg acgcccccaa atttggaaca gcaatccagg ggctaattca aggtctggcg
     1441 aatttctgac ccctaagaca ccttggctgt cgacgtaaat attcggccag atttgctcca
     1501 ctgtattttg aatcagtcca tccacctgtg aaatcaggct gaagctcact gctccgtata
     1561 ccgcagtgcc gaaaaggaaa agaattcccc ccacgataga agtgtagaga accgctaggc
     1621 gggtgcgtag agacattcaa gtttagtttg attcgcgaag tacatatccc atccccctga
     1681 ctgtatgaat caatcgaggc tcgttgatct cttccaattt ctgccgcaga tagcgaatat
     1741 agacctcgat aatgttgctt tcaccgccaa aatcgtagcc ccaaacccgg tcgaagatca
     1801 tatcgcgggt caacacctgc cgaggatggc gtagaaacag ctccagcaac tcgtactctt
     1861 tggcagtcaa agagatgatt cgctctcctc gggaggcctg acgcgtgcca gtatctaagg
     1921 agaggtccgc aaatcgcagt acttgaggtc ggctgggttg agagcgacga aggagggcgc
     1981 gaatgcgcgc aagcagttcg tctaagttaa aaggcttgac catgtagtcg tcggcgccca
     2041 tgtctaagcc catgatgcga tcgttgacag tgtccttggc tgttaggatc aatatcggta
     2101 caggcccgcc agccctgagc cgccggcata tctctagccc gtcgatgccc gggagcatta
     2161 gatccagcac gaccagatct ggcgggttat cgcgggcaat gttcaatcca atagtgccgt
     2221 ctgctgctgt gtcaacctga tagccctcat aagccaatcc ccgtctcaag aatttgagga
     2281 tagcctcgtc atcttcgatt actagaatac gcgagctcat actgttctcc tgttcacaat
     2341 ataccacaag gcattatcag aaaaatgtgg gtcatgggaa atgctgtggt gagaggttgc
     2401 cttatgcatg gaaatttgtc ataaacttaa taggtttatc ctgggattgc tgctcgaagc
     2461 gcggcaatat tctcctcaat gctcgcagcg gaattgaaga ccgatgtacc acacaccagt
     2521 acatttgcgc cagcccgcac gatctcggga acgttgcttg gacggatgcc tccatcgacc
     2581 tctacatcaa catgcgacag gccgcgggcg tccagcatgt gacgcaaacg ggtgatctta
     2641 tcagtgctgc tggagatata actctgtccc ccaaacccgg ggttgacaga catcaccaga
     2701 acgatgtcaa cagcggatat gatctcgtcc agagtgctta aagatgtggc gggatttagg
     2761 gtaacgcctg ccttcactcc catctccttg atatgctgga tggtgcgatg cagatgcctg
     2821 gaggcttcaa cgtgaactgt gatgttatcc gcgccagctt gcacatataa gggaactaga
     2881 cgatcgggct cttcaaccat caaatggaca tccaatatgg cgttagcctg ctggacgata
     2941 ggcttgatcg cggatacgat cattgggccg atagtcaaat ttgggacgaa gtgcccatcc
     3001 atgacgtcca catgaatata ttctgcccct ccgtcgaggg cctcctggac ctgattgccc
     3061 aagcgggcga aatcggctgc gaggattgaa gcggcaatct ttacatgcat cacaagtgaa
     3121 atcattatac tggattatgg ctcgaaagat ggacatcagg tcgatctttc gataatgcgc
     3181 gtgctttgtt cgccagcgat gatcaccagg gtggccatat cgaggaacag gccatgctca
     3241 acgatgcctg ggcgctcagc caggcgacgc gccaggctat agacatccga gatgccacgt
     3301 tcaaaccaac agcgcaccag gtaattatta ttgtccgtga tcacaggcga tccgtcttcc
     3361 tcaacccaaa gctctgcccg gcaacccagg gtgttcagcc agcgcacatg ggtttccgct
     3421 tcgaagggga tgatctccac gggcagcggg ccgcgagtgc ctagcctggg taccattttg
     3481 gtttcgtcca ccataatgat aagacgttcg gcgtgaatct ccaccacctt ctcgcgcagc
     3541 agggcgcgtc ccaggccttt gatcagattc aggctggggt cgacttcgtc cgccccatct
     3601 atggccaggt cgagtcgcgg ctcgccgcct gggttcgaag atgataagga gatgattgga
     3661 atactgaggg cgcgagcctg attggcagtg gcctcggaag tgggcacacc acggatctcc
     3721 cgcagtgagc cagatcgtat ttgctctccc agcaggtcta cgaaatgggc tgcagtagac
     3781 ccgctgccca gcccaagcgt catgccgtcg cgcacataat ccaatgcccg gcgggctgct
     3841 ttatattttg agttttggat ttcatgcttt gccactgcaa tatacctggg atccttgtgg
     3901 catccttttc ccattgaggg ggagaacaat tgcgttgggg cggcaatctt tgtccgccta
     3961 gcttacttta gggtcaacaa gaaagccaac aggtcgttga tttcgctttc tttcaagtct
     4021 ttggcgtagt tttggtacat tattcccgcc gaaaaaccat ccaccaggtg agcatcaggt
     4081 tcaatgatag actctcttag atagtcttca gccggcatgc ctgggacggc tgtttccgct
     4141 tttgtggcta gcccggcatg ggggggaccg atcagcacct tccccgcctc gagcgaatgg
     4201 caagtgatac aacccggcgc ggcctgcgac ccgataaccg tctgtttata gagctcttca
     4261 ccgcgtttcg cgtctccacc actaccgcca tcagcagtat ctgtgccacc gcatgcgacc
     4321 agcagtgtac tgagaactac gatagcgata accagagtga gagtacgttt tggcatttct
     4381 catactcctc tttaaataga tcaattgaat gttggactta tcccctcata cgtcaaagga
     4441 aaacattgtc ccaagccaga gtctaccaca gcaaatctac actccactta gaaaattccc
     4501 gaaatctaag acaatggggt tttgcgggta cttctactcc acaaaacccc atgggcgtga
     4561 ttaattttcc cttttcaggg taatatacct gtgggatgtg atgagcaaag ctattttact
     4621 tcgatcacag cgccggcggc ttcaagctta gacttggcgt ccatagctgc atccttgctg
     4681 acggcttcca tgactttgcc accaacggtc tcagcaagct ccttggcctc tttcaggccc
     4741 aggctgctgg agagctgacg gatggtcttg atgacctcga tcttctttgg accagcgtcc
     4801 ttgatgatga catcaaactc tgtcttctct tcctcagcct cggcaggggc acctgctgct
     4861 gcaggagagg ccatagcagc cattgccacg ggcgcggcag cggaaacacc ccaggcttcc
     4921 tctagttttt ggaccagctg ggaggcttcc aatacgctga gcttgctcag ttcttcaact
     4981 aatttatcga tattcgccat tacttaatct ccttattatt aatgaactct gataagtgat
     5041 cagcagtccg tgatttgtga tatgtttctt actcaatgcg atcttgcacg atgatattta
     5101 tgactaggct gcctcaggcg ccgattcgcg gtcggcgtaa gctttgacga ctgccgcgac
     5161 catgcgggcc ggctcggcaa gggtgcgggc cagcttgctg gctggagcca gcagcgtccc
     5221 caacaactgg gcacgtacca caggcaaggg gggcaattcg gcaagcgcct ttacatcttc
     5281 agcggtcatc gcttgtttgc cgagatagcc gcctttgatc ttgatgaact cggatgtacg
     5341 cgtaaactct gatatagcct tcgccaaggc gggtccatcg gtgaaagcaa aacccactgc
     5401 agtgctgcct tcaaagaaac cttccggcag aggcatgtcc tgatctttaa gagccaggct
     5461 cgccagtgta ttcttgacaa tgtgaaactc gccaccagcc tcacgggctt ttttgcgtag
     5521 ttcatccaac tgcttcatgg tgatgcctgt gtactcagtc aggatcagcg cctggcagcg
     5581 gtttgcccac tcgctgtact gggcaaccac ttcatgcttt cttttctttg tgatagccaa
     5641 tgtgtcttcc tcctttcaaa taaaagtctt tgcaccgcta ggcagggcaa agactcgcga
     5701 aagctcgatc tccatttgaa aacccgagat tgtcactaaa agcaggcaaa acttctgtct
     5761 acagtgtcaa gggttttcgg ctcggtttct gaattgcggt cttcaccttg gcaggatatt
     5821 aagctgtgcc ctggttaggg caagagcgcc tgctgtctct ggcgaagcgg tattatcgat
     5881 aacggatcgc gttactcgag cacctccatg gcttgcgcct ggttcgggtc caccttaatg
     5941 ccgggaccca tggttgtggt gagcgtgatg cgcttgatgt aggtaccctt agacgccgct
     6001 gggcgggctt tctttacagc ctccatcagc gctgccaggt tctcatagag tttgttctgc
     6061 tcgaaagaca cttttccgat cggcacatgc aggttggcgg ttttgtccag gcggaactca
     6121 acccgaccgg ctttagattc gttcaccacc cgcgggatat cttcagcttg gacaacggtg
     6181 ccggccttgg ggtttggcat caagccgcgc ggacccaaaa cccggcccag acgacctacc
     6241 ttccccatca tatcgggtgt ggcaatagcc acatcaaaat cagtccagcc tgcctgaatc
     6301 ttggcaaggg tctcatcatc gtcggcgacc aagtctgcgc cggcctcccg tgctagcgag
     6361 gcgccttcac cctgggcgaa aaccaggact cgcaccgttt tacccaggcc atggggcagc
     6421 accaccacgt cgcgcacttg ctggtcggca tggcgaggat caacgccaag tcgcatgtgg
     6481 atttcgacag tgccgtcgaa cttggatata gatgtctcct tggcgagagc aactgcttcg
     6541 gcaggggtgt agagttgttc cttatctact ttggcggctg cagccaggta tttctttccg
     6601 tgtttcgtca tgaatcactc cttcgtggtg caagcgggca gtatgctgcc ctcccaccag
     6661 gtcaatcgat aatctgtata cccatattgc gggcggtacc ctcgatctgg cgcatagcac
     6721 cttcgatatc cagggcgttt aggtctttca tttttagctc tgcaatctcg cgaacctgag
     6781 cgcgggtgac cttgcccact ttttcgcgat tgggctgagc ggatccttta tcgaccccgg
     6841 cagcttttct caataaaaca gctgcgggag aagtcttgag gatgaacgtg aacgacccat
     6901 cggtgtagat gctgatctct gcaggaatga tttcgcccat gcggcttgaa gtgcgggcat
     6961 tatattcctt gcagaatgcc atcaaattga tcccgtgccc agctagggcc ggaccgatgg
     7021 ggggcgcagg gttggcctta ccagcctgta tctgcaatcg gatgatagtt ttaagtttct
     7081 ttgccaacgt accttctcct tcgtggtaaa cgggtgatgg gggaacaccc tcccaccttt
     7141 atagccattc gccgccagcc tgaaaatggg tgctgatggt cgcaggctaa ttgcttctat
     7201 gccttttcta cttgcagaaa gtccagctca accggagttt cgcggccgaa gaagttaacc
     7261 atgacacgca ccttggctcg ttccatatcg atttcggaga ccgtaccacg aaaatcgttg
     7321 aaggggccat ccacaatacg cacacgctcg ccggatttaa atgtaacctt gatacgtgga
     7381 gcctcggctt ccatgcggcg gatgatttgc gcgacttcct cggggcggag tggcgtgggg
     7441 ttgttgccca tgccaacaaa tccggtcacc ccaggtgtgt tgcgtacaac ataccaagat
     7501 tcttcgctca aaatcatgtt gaccaggatg tagcctggga aaacccgccg ctctactgtt
     7561 cggcgcttgc cttccttgac ctcgatctct tcttcagtgg ggacgacgat atcgaaaatc
     7621 ttgtccttca tccccatggt ttcgatgcgc tgctccaggt tgtggcgcac cttattctcg
     7681 taaccggaat agcaatgcac tacataccag gcgcggtcat cttcagcctg gtcttcaatc
     7741 tcaaccggga cgtccaggct ttcttccgaa tccaacgatt cggccgagag ttcgtcttgc
     7801 ggcgtttcga cctcagtgat ttcgtcttcg gtcatcaaag gttgctcttc cggaactttc
     7861 tcttccataa aaatactact ctctcgcctg ccaattaata ttttatcagc ttgctttcaa
     7921 acaatataat tctacccaag gatcagtgcc attaaggtcg taaagatata atccaggacg
     7981 ccaaggtata atcccatcac taccatcaca accaaaacga tcttggtcag attccaggcg
     8041 tcctgccagg tgggccagct taccttgcgc aattcaccaa ccgtctcacg aaagaaaagc
     8101 tggattttgt tgctcgacct gcgctctata gattttccct tatcagcttt ggccattacg
     8161 gcctccttca tggggtcaca ctactaaaga taaagccggt gaccctcgcc agtattattt
     8221 gttacggaca aaacgccgcc ccggtagacg gcgttgcctt ttttatggca ggccaggcag
     8281 gacttgaacc cacaaccgct cgttttggag acgagagctc taccaattga gctactggcc
     8341 tgttttatgc gctccagagt cacgtcttat cttaccatgt gaccctggag cacctggcac
     8401 tttacttcgt ctcgcgatgt aaggtatgct tgcgacagcg cgggcagaat ttatttaact
     8461 ccatgcgacc cggatcattg cgacggtttt tctcggtggt gtaagttcgt tccttacact
     8521 ccgtgcaagc cagcgtaatt actgggcgga tatctttctt agatgccata gcttatctat
     8581 taatccagaa tcttagtaat aacgccggcg ccgacggtca agccaccttc acggatggcg
     8641 aacttggagc cctgctccag cgccaccggc acgatcaact ccacttccag gttgacattg
     8701 tcgcccggca tgaccatctc cacgccttcc ggcagggtga tattcccggt cacatccatg
     8761 gtgcggatgt agaactgcgg ccggtacccc gtgaagaacg ccttgtgccg cccgccttct
     8821 tccttgcgca
//
LOCUS       DBNGSX010000536         4044 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1024848, whole genome shotgun sequence.
ACCESSION   DBNGSX010000536 DBNGSX010000000
VERSION     DBNGSX010000536.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1024848"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="AC16CB_15850"
     CDS             complement(<1..767)
                     /locus_tag="AC16CB_15850"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR3343996.1"
                     /translation="MSFGPIYIAGPERSGTSLIYALLASHPNIAMTRRTNMWTHFFNQ
                     YGDISRPKNFERCLSMMMRYKRLLLLKPDPERIRREFWQGEQTYARLFALLEEHYAEQ
                     VGKPRWGDKSLNTERYADAIFAAFPEARILHMVRDPRDRYSSALTRWKVSRGGVGAGS
                     AIWLSSIKLAQQNQEKYPDQYKIIRYETLVNQPEETMREICAFIGEEYTPAIFSMDGA
                     KTFRDEGGNSSYGQHEPGRISNKSIGRFRQVLSKRQIA"
     gene            complement(844..1773)
                     /locus_tag="AC16CB_15855"
     CDS             complement(844..1773)
                     /locus_tag="AC16CB_15855"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR3343997.1"
                     /translation="MSNGPVFIGGLSFSGKTQLRLLLSSHPNFVFTRRTYMWTRFYER
                     YGDLSQAENLERCLNAMMRSKHIQALNPDLDRIRREFWHGPPDYPRLFALFHEHYAES
                     QGKTRWGDQLGFVEKYADPIFAAYPSAKMIHMIRNPIERCQESISTKAYRKGKVGFET
                     AHWMRSARLARQNQQRYPDRYKIVLCEELFARPEETLLDVCHFLGEEYFPYMLEESSK
                     PGFWKEAGSYRDGSTEVDDHPSNLSKNGQPSLSKTEQGFIHAIAGREMIAFGYPAEVY
                     PFTAKDWLLYLFIDWPANLAGMAAWRSRVGKSH"
     gene            complement(1822..2865)
                     /locus_tag="AC16CB_15860"
     CDS             complement(1822..2865)
                     /locus_tag="AC16CB_15860"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR3343998.1"
                     /translation="MMKTMGVGELLGDRGPVFIGGIQRSGKTYLRFMLSSHSPLVFTR
                     RTNMWPNIYNRFGDISQVGNFERCLAAMMQNKHVRALEPDIELIRQEFWHGIPSYARL
                     FALIHKHYAEQQGKLRWGDQTELIERFADLILDAYPDAKIIHMIRDPRDVYEASLARN
                     PLDKKKVGDLTARWLYSASLATRNQRVYPDRYKIVRYESLISQPKETLGDVCRFLGIE
                     YKPAMLRMEDVPRFWKLDEEYSNIAGTPLNTDYIGRFRKSLSVYEIAFIQKYAGGFFS
                     QYGYSLEIIRFSTIEKLRFYLIKGGINLARLRSWHFRNLVLRKLISKIISRKKYSKVD
                     YRTNKRLSKPEAI"
     gene            complement(2862..3884)
                     /locus_tag="AC16CB_15865"
     CDS             complement(2862..3884)
                     /locus_tag="AC16CB_15865"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR3343999.1"
                     /translation="MGKGPIFIGGTDRSGKTTLRAFLVSHPNISIPANGSNMWSYFYG
                     QFGDLSQAENFERCLDALMHYKHALYLKPDPDRIRHEFWQGTPTYARLFALFHEHLAE
                     REGKPRWGDQTGLIERYADQIFEAYPNAKMIQMIRDPRDRYQASLTLWPKGKGRAGGA
                     TARWLYSTSLAIRNLRRYPNRYMIVRYETLVRDTEQTLREVCTFLDEDFIPSMITMDG
                     DLDRRSRLTQGLNIKPDTNPLTPDFIGLYHQAVPQKEIAFIQGIAGKQMKAIGYSLDA
                     ISFSLSDRIRYILLDWPLNMLRMLAWLSLEIAHHNFPAQFGRKLSSNMITPRKHQNNK
                     VNARAT"
BASE COUNT         1029 a          947 c          967 g         1101 t
ORIGIN      
        1 gcgatttggc gtttagataa tacctgccga aaacgcccga ttgatttgtt tgaaatgcgg
       61 cctggctcgt gttgtccata ggagctattg ccgccctcat ctcgaaaagt ttttgcacca
      121 tccatgctga agatggctgg ggtgtattct tcaccgataa atgcgcaaat ttcgcgcatc
      181 gtctcctcag gttgattgac taaggtttcg taacgtatga tcttatactg atcaggatat
      241 ttttcttggt tctgctgggc aagttttatg gatgagagcc atattgcgct tcctgcgccc
      301 acacctcccc ggcttacttt ccagcgggtt aatgctgatg aatatctgtc gcgtggatca
      361 cgcaccatgt ggagaatcct ggcttcggga aaagctgcaa atatggcatc tgcatagcgc
      421 tctgtattca atgatttatc cccccagcgt ggtttgccta cctgttccgc ataatgctcc
      481 tcaaggaggg caaataagcg ggcgtaagtc tgttcacctt gccaaaactc gcgacggata
      541 cgttctgggt ctggtttgag tagcaacaag cgcttgtaac gcatcatcat ggagagacaa
      601 cgctcaaaat tctttggtcg gctgatgtct ccatattgat tgaaaaagtg agtccacatg
      661 ttggttcgtc tagtcatcgc gatattaggg tgggatgcta ataacgcgta gatcagactt
      721 gttccacttc tctctgggcc ggcaatgtat attggtccaa aactcatgta tcttcctcca
      781 gtttctttct agacgacaaa caaaataagt gcaataaaag aaagctcaat cctgaacgca
      841 aggtcaatgg cttttaccaa ctcggcttcg ccaagctgcc ataccagcca ggttagctgg
      901 ccaatcgatg aataagtata aaagccagtc cttcgccgta aatgggtaca cctctgccgg
      961 ataaccaaag gcaatcatct cccggccggc tattgcatgg ataaagccct gttcagtttt
     1021 tgagagagat ggctgaccgt ttttgctcag attggatgga tgatcatcga cttcagtact
     1081 gccatctcgg taactgcccg cctccttcca aaatcctggc ttgctgctct cctctagcat
     1141 atatggaaag tattcctcgc ccagaaaatg gcaaacgtca agcaaggttt cttcagggcg
     1201 agcgaatagc tcttcacaaa gcacaatttt gtaacgatct gggtagcgct gctggttttg
     1261 ccgggcaagc ctcgcagagc gcatccagtg ggctgtttca aaaccgactt tgccctttcg
     1321 atacgcttta gtggatatac tctcctgaca cctttcgatt ggatttctaa tcatgtggat
     1381 catctttgcc gatggataag cggcaaagat aggatcagca tatttttcta caaaacctag
     1441 ttgatccccc cagcgtgttt tcccttgaga ttcagcgtaa tgttcatgga agagagcgaa
     1501 taagcgcggg taatctggcg ggccatgcca gaactcccgc cggattcgat cgaggtctgg
     1561 gttgagagct tggatgtgtt tggacctcat catagcattc aggcagcgct ctagattttc
     1621 tgcctggctc aggtcgccgt agcgttcata gaagcgcgtc cacatataag tgcgccgggt
     1681 gaagacgaag ttagggtgcg aagagagcaa caagcgcagt tgagtcttac ccgagaagct
     1741 caatccaccg ataaaaacag gtccattgct catactgata taccgttgcc tgtgcaggcg
     1801 cgaaggcaaa ccaaaagaat atcagatggc ttctggcttg ctaagtcttt tattggtgcg
     1861 atagtcaacc tttgaatact tcttccttga gatgatctta gaaatgagct tgcgtaaaac
     1921 caggttgcga aagtgccagc ttcgcaagcg ggctaaattt attccccctt tgataaggta
     1981 aaagcgtaat ttttcaatgg tcgaaaaacg gataatctct aaggaatagc catactgaga
     2041 gaaaaatcca ccagcgtatt tttgaataaa ggcaatctcg tatacagaaa ggcttttccg
     2101 gaaacgcccg atgtaatccg tattcagggg agtgcctgca atattgctgt attcttcatc
     2161 tagtttccag aaccttggca catcctccat tctcagcatc gctggtttat actcgatccc
     2221 caaaaaacga cacacatcac caagcgtttc tttgggctgg gagatcagcg actcgtaacg
     2281 cacaatctta taacggtcag ggtaaactcg ttgatttctt gtcgctaagg aagcagaata
     2341 gagccagcgt gcagtaagat cgccgacctt ctttttatcc agtgggttgc gagccaagga
     2401 cgcttcataa acgtcgcgcg ggtcgcgaat catgtggatg atcttagcat ctgggtatgc
     2461 atcaaggatt agatcagcaa agcgctcgat gagctctgtt tggtcccccc aacgcaactt
     2521 gccctgttgc tctgcataat gcttatggat aagtgcaaat aggcgagcat aagatggaat
     2581 tccatgccag aattcttggc gtatgagttc gatgtctggc tcaagagcac gtacatgttt
     2641 gttctgcatc atcgcggcca agcaacgttc aaaattgcct acctggctga tatcaccgaa
     2701 acgattgtag atattcggcc acatgtttgt ccgacgggtg aataccaggg gggaatgcga
     2761 tgagagcata aatcgcaagt atgttttgcc agagcgctgg attcctccta tgaaaactgg
     2821 cccgcgatcc cctaacaatt cacctacacc catcgtcttc atcatgttgc ccttgcattg
     2881 accttgttat tttgatgttt gcgcggggtg atcatattgg agcttagctt acgtccgaac
     2941 tgagctggaa aattatggtg tgcgatctca agggataacc atgctaacat gcgcaacata
     3001 ttaagtggcc agtcgagaag aatgtatctg attcgatctg agagtgaaaa acttatcgca
     3061 tccagagagt aaccgatagc tttcatttgc tttccggcaa tgccctgtat gaaggcgatc
     3121 tctttttggg gaactgcttg gtggtaaaga ccaataaagt ctggcgttaa tggattcgta
     3181 tcaggcttga tgtttaaacc ttgggttagc ctagatcgac gatcgaggtc gccatccatg
     3241 gtaatcatgc ttggaataaa atcctcatct aaaaaagtgc agacctcacg caaggtttgc
     3301 tcggtatcac gcaccaatgt ttcatatctg acgatcatat agcgattagg atatctgcgc
     3361 agattgcgga ttgcaagaga cgtcgaatag agccatctgg ctgtagctcc gcctgcccgc
     3421 ccctttccct ttggccagag agtcagcgaa gcctggtagc gatcacgtgg atcacggatc
     3481 atttggatca tcttcgcatt tggatacgcc tcaaatattt gatctgcata ccgttcaatt
     3541 aagccggttt ggtctcccca gcgaggcttg ccttcgcgtt cagcgagatg ttcgtgaaat
     3601 aatgcaaaca gacgggcgta ggttggtgtc ccttgccaaa actcgtggcg gatgcgatcg
     3661 ggatcaggct tgagataaag tgcgtgttta tagtgcatca gcgcgtccag gcaacgctca
     3721 aaattttcgg cctggctcag gtcgccgaat tgaccataaa agtacgacca catgttagaa
     3781 ccattggcgg ggattgaaat attcgggtgt gatacgagaa atgctcgcaa agtcgtcttc
     3841 ccggaacgat ctgtcccgcc gataaatata ggtcctttac ccatagtttg atttcctaaa
     3901 tttttagggg ttttcagaat tacttccccg agttttttga gaacaattta tttgctcgtt
     3961 gccgtatgaa cccaataacc gcttgatcct caggagagaa tcccatcagt agggttatac
     4021 ccaaatacac agctagtaat aagc
//
LOCUS       DBNGSX010000537         2901 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1717410, whole genome shotgun sequence.
ACCESSION   DBNGSX010000537 DBNGSX010000000
VERSION     DBNGSX010000537.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1717410"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            452..>2901
                     /locus_tag="AC16CB_15870"
     CDS             452..>2901
                     /locus_tag="AC16CB_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344000.1"
                     /translation="MMLNFALPLGSVQEVFADSVNSLPAAAHTPQNQPDQYINRSVDP
                     TTEVSPALLDASDQAQIPFAAIDSRNLPVDLREIPERFDMRTANSATFQDPGGIFQTV
                     ISPDTMHYLDELGNWQVIEPVFQQLDDSFVVQRNSLKSRAGARRAWMSGMVGETAAIW
                     EASELGVAGVDGNFTSLAKALAETPYPAERQLAGRLLHYVGGWSDPTLVEEIASAPDA
                     LEHSLILGERPQIDVKDGYLEMRATLDLLPGASLWVDGEKVDGEARTGGMIEIRDAAG
                     KPALALSPVVAFEQANPMITVDGEYLISPGDELGSWQVRVRTPLAWWLEPARQYPLVI
                     DPEMRVLKSTGYADGMGWVRSNDTKAFTLGGIRLGAHLPYWDTKSRGYVQFNSLPSLL
                     TNSSLQVLSASLDVTPTLPPEAMPLYKNSPKDWEHEKISRNTELHYLGACPDTSGCNG
                     LSFLTGAPSNNWDNSPEGTLVGTKLLKAGPASGGGTPQTTTWDVTTQIQSWYTNHFTD
                     NTQRPGPTFMMRFPDTTKENCPAPGPYYFGFVPGKVNEINTSWQYVPYCIWFDIPAGS
                     VQLRIAYQSLALKINDNLLNTPGVPSFLEGTFENTNHQYMLTPAVAIQKHWRAVAVRG
                     NHDFDEALPTRVGLQVSDYTTDPSNPELLASGVTKGPDETAFVFLDDHAPANLGLSMV
                     DLRAEVTASEQPPPNLEGDYLSDLSRNYRIEYEEAQNQTFTYGQWSTLGWGEFPTDQL
                     ISLLEFTLNEGDNFIIRASAPITLELALIEPSGGTIRADAVLGSNDAKVNRSFSLKDS
                     VERSIFVGSATGGKWAVAA"
BASE COUNT          669 a          752 c          796 g          684 t
ORIGIN      
        1 gatgacaatt taataactga gtcaattgta aggaaatttg aaggattacc cattgacatt
       61 gcaactatct tcatctaaaa tccgtatatt atcacaattg gctgatttgc gctcctactc
      121 taaggaagaa tccatttcgc cagtccgttg gtagggggat gtattttgga cagcattttc
      181 tatttcaacc attgcaagag atgcaggact tctctcgata aaattcaata ggctagataa
      241 gttctgaggt tatgacggtc gatagcttgt tctaaggtag tgttcagtaa ttccgatctt
      301 attctcagca cgcggggtgg tcgccatgaa aggattcaat ttattcaata caggtaggtt
      361 aaatccaatg actaagctat tcttgcggca attttttttc aggcttgtga aacgaggtcc
      421 ggcgcttgtt taccggatct ttgtattttt tatgatgctg aattttgcct tgccattggg
      481 atctgtgcaa gaagtgtttg cggactcggt aaactccctg cctgcagctg cccacacacc
      541 tcaaaatcag ccggatcagt atatcaatcg atctgttgat ccgactaccg aagtatcccc
      601 ggctcttctg gatgcctctg atcaggcgca aatacctttc gccgccatcg actcgcgtaa
      661 tctacctgtg gatctgaggg agattccaga gcggtttgac atgcgtacgg ccaatagcgc
      721 tactttccaa gatccagggg ggattttcca aacggtaatc tcccccgata ctatgcatta
      781 cctggatgaa ctcggcaatt ggcaggtgat tgaaccggtc ttccaacagt tagacgatag
      841 tttcgtggtg cagcgcaatt ccctgaagag ccgtgcaggg gcgcggcgag cctggatgtc
      901 ggggatggtg ggggagacgg cagccatctg ggaggcatcc gagttagggg ttgctggcgt
      961 tgatgggaac tttacctcgt tagcgaaagc gctggcggaa accccttacc ccgcagaacg
     1021 gcaactggct ggcaggctac tgcattatgt gggcggctgg agcgacccaa ctctggtaga
     1081 ggagattgcc agcgcgcccg atgccctgga acactcgttg atcctggggg agcgaccgca
     1141 gattgatgtc aaggatggat acctggagat gcgcgctact ttggatctgc tgcccggcgc
     1201 cagcctgtgg gttgacgggg aaaaggtgga tggggaggcg cgtaccggcg ggatgataga
     1261 gatcagagac gcggcaggga agccagccct ggctctctcc cccgtagtgg cttttgagca
     1321 ggctaatcct atgatcactg tggatggtga atacctgatc tcccctgggg acgaattggg
     1381 tagctggcag gtgcgcgttc gcacgccatt ggcgtggtgg ttggaacctg cccgccagta
     1441 tccgctggtg attgatcccg aaatgcgggt gctgaagagc acaggctatg cagacggtat
     1501 gggttgggtg cgcagcaatg acaccaaagc cttcactctg ggcggtatcc gcctgggggc
     1561 gcacctgccc tattgggata cgaaaagccg ggggtacgtg cagttcaaca gcctgccatc
     1621 cttgctgact aattcgagcc tccaggtgct gtcggcttcg ctagatgtga cgcccaccct
     1681 gccccccgag gcgatgccgt tatataagaa ttctcctaaa gattgggagc atgagaaaat
     1741 ctcccgcaac accgagcttc actatttggg cgcttgcccg gataccagtg gctgtaatgg
     1801 actgtccttc cttaccggcg ctccctccaa taactgggac aacagcccgg aagggacact
     1861 ggtcggcacc aagctcctga aagctgggcc ggcgtctggt gggggtacac cccaaacaac
     1921 cacctgggat gtcaccacac agatacaaag ctggtatacg aaccatttta ccgacaatac
     1981 acaacgccct gggcccactt tcatgatgag gtttccagac acgacgaagg agaactgccc
     2041 agcgccaggc ccttattatt ttggctttgt ccctggtaag gtcaatgaaa ttaacacatc
     2101 ctggcagtat gtaccttatt gtatctggtt cgatatcccg gctggaagcg tgcagcttcg
     2161 tatagcctat cagtccttgg ccttgaaaat aaacgataac ttgctgaata ctcctggtgt
     2221 gccgagcttt ttagagggca catttgaaaa taccaaccac caatacatgc tgactccagc
     2281 cgttgcaata caaaagcact ggcgtgcggt tgctgtgcgc ggcaaccatg actttgatga
     2341 ggccctaccg acccgcgtag ggctccaggt aagtgactac acaaccgacc cgagcaaccc
     2401 cgaattgctc gccagtgggg tcaccaaagg accagatgag accgcgtttg ttttcttgga
     2461 tgatcatgcc cccgccaacc tggggttgag tatggtagac ctgcgcgccg aggtgaccgc
     2521 ctccgagcaa cccccaccca acctagaggg cgattacctc tcagacctat cccgcaacta
     2581 ccgcattgag tacgaggaag cccaaaacca gaccttcacg tatggacagt ggtctactct
     2641 cggctggggg gaatttccaa ctgaccagtt aatatcgctt ctggaattca ccctcaacga
     2701 gggggataac ttcattatca gggccagcgc gccgatcacc cttgagctgg cgctgattga
     2761 acccagtgga gggacaatca gggcggacgc ggtcctaggt agcaatgatg ccaaggtcaa
     2821 ccgcagtttc tcactcaagg actcggtcga gcgttcgata tttgttggct ctgctaccgg
     2881 tggcaagtgg gctgtggcag c
//
LOCUS       DBNGSX010000538         6557 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_262836, whole genome shotgun sequence.
ACCESSION   DBNGSX010000538 DBNGSX010000000
VERSION     DBNGSX010000538.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6557)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6557)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6557
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_262836"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="AC16CB_15875"
     CDS             <1..1059
                     /locus_tag="AC16CB_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR3344001.1"
                     /translation="PKGVSGPVSPPAAGEFLVGVVLPTRDEPRWIQDETRFKDAFAAA
                     GYDVQILFSEGDSAKEKANVEALIAQGVKVIVICPQDATAAAAAASEARAAGVKVVSY
                     DRLIRDTDAVDYYVTFDSIAVGEAQAQYLVDKASGSGNPLYLYAGAATDNNAFLFFEG
                     AWNVLQPKIVDGTFVIKNSSEAIALQDKATLTRDEMSKIIGQVTTDWKFDVAKNLAEA
                     NLTATAAADKGDVFILAPNDGTSRAIADVFAADADVTSYVISGQDAEVASVQYIIDGK
                     QSMTVLKDVRTLVADAIAAAIAYLEGGAPEQTNTYNNGVIDVPAKPSVVITVDKDNVV
                     EAVIDSGYWDASEFTGLP"
     gene            1254..2057
                     /locus_tag="AC16CB_15880"
     CDS             1254..2057
                     /locus_tag="AC16CB_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3344002.1"
                     /translation="MVSVSESAPVVEMRNIKKNFAAVQALRGVDLVLHHNEVLGLLGD
                     NAAGKSTLMKVLSGAYIPDEGEIILDGEKVQITDPSHARRLGIEMVYQDLALANNLNV
                     AANVFLGRELVGTQFGPVGVMDSHHMEVETQRLLDRLKIDIPSVRLLVERLSGGQRQA
                     VAIARATAFNSKVTIMDEPTAALSVAAISKVLDLVRELKAQGASIIIISHRLEDIYQV
                     SDRMIVLRHGRKVGDCPVEGDIDEFRERVVAYIVGARDDFANKEVGSVK"
     gene            2078..3262
                     /locus_tag="AC16CB_15885"
     CDS             2078..3262
                     /locus_tag="AC16CB_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HIR3344003.1"
                     /translation="MSYFKDLQKLFQQNIREYGMYIAFFVIIVFFSFTTKGLFVSSRN
                     IGNLVNATSYIAVLAVGMTLVIVIRHIDLSVGFLAGFLGAIAAISMKFWLNPSYLTIP
                     LILGLGILAGLLTGFLVAQMKIPAFVATLAGSLGYRGALQLVTSSTGTIIISDDMFNA
                     IGNGFIPDIPGLEILPEIHKLTLLLGLGAIILFIIGEINSRRKTLSYDFEVLSMSMFI
                     TKLVFVSAVIGGITWVLAGYQGLSWAAVVLLAVVMIYSFITTKTVLGRHIYAVGGNPE
                     AAELSGISVKRITYLVFGSMGMLAALSGILYASWLRSATTIAGTLFELDAIAAAFVGG
                     VAVAGGVGKVTGSIIGALVMASLTNGMNLMGVGISLQYIVRAAVLAAAVIFDVVTRNA
                     GK"
     gene            3591..4754
                     /locus_tag="AC16CB_15890"
     CDS             3591..4754
                     /locus_tag="AC16CB_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3344004.1"
                     /translation="MDNLGRAEVRRQNLVAQVVSFIGRVLLAVAIPAIAFYVLYQGFL
                     FLRDSQAPRSLIALVAIVWGVGGVAILYWIFNSLVEILPDEWTARLQPFVFVGPAMAI
                     LTWYLALPTVRTFWISLFDRGGPPDGLSILEQMRSASFVGLSNYVAVFTERLMIEAFR
                     NNIMWIVFGSTLSVTFGLIVAVLADRSRFEKVAKSLIFLPMAISFVGAGIIWNFIYEY
                     RPANQPQVGLLNSIVQGFGGQPRAWQQWVDIIPWNNLFLIVIVIWLQAGFAMVLFSAA
                     LKGIPEELIEAGRIDGANEFQIFFRIMIPYIQGTLITVWTTIVIFTLKIFDVVWVMTG
                     GQFGTHVIATQFYRQSFTADNKGFGAAIAIILLIAVIPVMIYNLSQFRQQEAF"
     gene            4756..5859
                     /locus_tag="AC16CB_15895"
     CDS             4756..5859
                     /locus_tag="AC16CB_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014370404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3344005.1"
                     /translation="MGKPTTQRRLGSLFVNGTLFLLVLIWTVPTLGIFVSSFRNRNDI
                     STSGWWNVFPHREWQPVAEIDTKATGLDPDGIMEIEGATGTFEEFRVGIETPDGKRIN
                     WIGNKRLGRVEIQERVWAVNWNFSLDNYNQVWGGQDFEFTKPDGTVEVVPGDDMSRAF
                     FNSLAVSIPSTVIPILIAAFAAYAFAWMRFPGRRWMFILVVALLVVPLQIALVPILRD
                     YKELELNGTFLAIWLAHAGFGLALATYLLYNYISALPREMLESAFIDGASHFTVFLRL
                     ILPLSVPALASFAIFQFLWVWNDYLVALVFLGGNPEFELVTQRMAAIVGARGQDWHLL
                     TAGAFVSMALPLLVFFSLQRFFVRGLLAGSVKG"
     gene            5991..6479
                     /locus_tag="AC16CB_15900"
     CDS             5991..6479
                     /locus_tag="AC16CB_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344006.1"
                     /translation="MPEQKIFSFDWALCFQWLMATTVGWVLGRFLLPNLAFITIGIGL
                     GVLQWLILQYRIKHAQQWILATIFSWTLGSLVILFLMPDVSDFIAGLVIGVATGTGQW
                     LVLRRELHWAGWWIPINVVAWTTGMALLPGVILTGIMAGLVTGIAIELLLRTPRNLVE
                     VK"
BASE COUNT         1426 a         1565 c         1816 g         1750 t
ORIGIN      
        1 ccgaaaggtg taagcggtcc agttagccca ccagcagcgg gtgagttttt ggttggtgtc
       61 gtacttccga ccagggacga gccgagatgg atccaggacg agacccgctt caaggatgct
      121 ttcgcagcag ccgggtacga cgtccagatt ctcttcagcg agggcgactc cgccaaagag
      181 aaagccaacg ttgaggcttt gatcgctcaa ggtgtcaaag tgatcgtcat ctgcccgcag
      241 gatgccacag ccgccgccgc ggcagcatca gaagctcgag cagcaggcgt gaaggttgtc
      301 tcctatgacc ggttgatccg cgacacggat gcggttgatt actatgtgac cttcgatagt
      361 attgccgtag gcgaagccca ggcgcagtac ctggtggaca aggccagcgg cagcggcaac
      421 ccgctgtacc tgtatgctgg cgccgcgaca gacaacaacg ccttcctgtt cttcgagggc
      481 gcctggaacg tgctgcaacc caagatcgtc gacggcacct tcgtgatcaa gaactccagc
      541 gaagcgatag cgctgcagga taaggccact ctgacgcgcg atgagatgtc caagatcatc
      601 ggacaggtca ccaccgactg gaagtttgat gtagccaaga acctggccga ggccaacctg
      661 actgccacag cagctgcaga caagggcgac gtcttcattc tggctccaaa cgacggcaca
      721 tcccgcgcca tcgcagatgt cttcgctgct gacgccgatg tcaccagcta tgtcatcagt
      781 ggtcaggacg ctgaggttgc ctcagtccag tacatcatcg atggcaagca gtcgatgact
      841 gtgctgaagg acgtgcgcac gctggttgcg gatgccatcg cggctgcgat tgcatacctg
      901 gaaggcggcg cgcctgagca gaccaacacc tacaacaacg gtgtgatcga cgtcccagcc
      961 aagccttccg tggtaatcac ggtcgataag gacaatgttg tggaagccgt gattgactcc
     1021 ggttactggg atgccagcga attcactgga ttaccgtaag atctgacagt tcgtgaacat
     1081 aggaatagtg atgggtaaaa ccatcactat tcctatattt cgtatattac tcaatctttg
     1141 acaaacacat ttgtaaaaca atcgcttttg aattaaacta tctattaagc agaaaaagag
     1201 gcgtcatcgt ctaagatcga ttctttataa atacaattct caaggagaaa gcgatggtat
     1261 ctgtgtcaga atccgcgcct gtggtggaaa tgcgcaatat caaaaagaac tttgctgctg
     1321 tccaggcgtt gcgcggggta gaccttgtat tgcaccataa tgaagtattg ggtttgctag
     1381 gcgataacgc ggctggcaaa tctacattga tgaaggttct cagtggggca tacatccctg
     1441 atgaaggaga gatcatcctt gatggcgaga aggtccaaat cactgaccct tcgcatgcgc
     1501 gccggctggg catcgagatg gtctatcagg acctggctct agccaacaac cttaacgtgg
     1561 ctgccaatgt tttcctggga cgagagttag tagggaccca atttggtccg gttggggtaa
     1621 tggacagcca tcacatggag gtggagaccc aacgcctcct ggatcggctt aagatcgata
     1681 tcccatcggt aagattgttg gtagagagac tgtccggggg acagcgtcag gcggttgcca
     1741 tcgcccgtgc aacggctttc aacagcaagg tcaccattat ggacgagcca acggcagcct
     1801 tgagcgtggc agccatcagc aaggtgcttg atctggtgcg tgagctcaag gcgcagggcg
     1861 catcgattat catcatcagc caccggttgg aagacatata ccaggtgagc gaccggatga
     1921 tcgtgctgcg ccacgggcgc aaggtaggcg actgtccagt tgaaggcgac attgatgagt
     1981 tccgtgagcg cgtggtggct tacatcgttg gcgcccggga tgatttcgca aataaagaag
     2041 ttggctcggt taaataaagc aggtgggagc gctcaagatg tcttacttca aggatctcca
     2101 aaaattattc cagcagaata ttcgcgaata cggcatgtat attgcatttt tcgtgatcat
     2161 cgtattcttt agctttacca caaaagggct tttcgtttcg tcaagaaaca tcggcaacct
     2221 ggtaaatgcg acgagttaca tcgcagttct ggcggttggt atgacgttgg tcatcgtcat
     2281 ccggcatatt gatctttcgg ttggttttct tgcgggcttt ctcggtgcga ttgcggcgat
     2341 ttccatgaaa ttctggctca atccctccta cctgactatt ccgcttatcc tggggcttgg
     2401 tattctagcc ggcctgctca ccggtttcct ggttgcgcag atgaagatac cagcctttgt
     2461 tgcaacgcta gccgggtcgc tgggttaccg gggagcgctg cagttagtaa cctcgagtac
     2521 gggaacgatt atcatctcag atgatatgtt taacgctata ggcaatggtt ttattcccga
     2581 cattcccggc cttgagatat taccggaaat acacaaattg acccttctcc tcggattagg
     2641 agccattatc ttattcatca taggcgaaat caacagccgc agaaagacgc tatcttatga
     2701 ctttgaagtt ctttcgatga gcatgttcat caccaagtta gtattcgtgt cagcggtgat
     2761 cggaggcatc acctgggtcc tcgccggata ccagggtctc tcctgggctg cggttgtctt
     2821 actggctgta gtaatgattt attctttcat cactactaag acggtgctcg gcaggcatat
     2881 ctacgccgtt ggcggaaatc cagaggctgc agagctaagc ggtatcagtg ttaagaggat
     2941 tacctatttg gtttttggtt cgatgggaat gcttgccgcc ttgtctggta tcctttatgc
     3001 atcctggctg cgttctgcca ccacaatcgc tggaaccctg tttgaattgg atgccatcgc
     3061 tgcggctttc gttggaggcg tagcggtggc gggtggcgtc ggcaaggtca ctggatcgat
     3121 cattggtgct ctggttatgg cgtcattgac aaacggaatg aacctgatgg gcgtcggcat
     3181 atccctccag tatatcgtta gagccgctgt acttgcggca gctgttatat ttgatgttgt
     3241 gacgcgcaat gctggcaaat aaaaactcta ttcaacgacc ggcatgaagt gtgggcgctg
     3301 atggaatatt taaccatgca gcaatctgct tctggttggc tgcagaacgg tggtggtgcg
     3361 ctggcggcgc accaaacagc cacccttgat atgtagggta taaacccgga accgaggtat
     3421 tgccgaattg gtacagcaag cgaccagctt gcgctttgac ggctctgatc tgatgcttgg
     3481 cgaaagtggc gctggccttc ccgggttggc cccttgcacc tgggctggta gtcagccacg
     3541 cattttatgg agcggatcct gactacaaat aatttataag gagtagactt atggacaatc
     3601 ttggcagagc agaagtacgt cgtcagaatc tggtggcgca ggttgtttcc ttcatcggtc
     3661 gggtgttgct ggctgtcgct attccggcga ttgcttttta cgtgttgtat caaggctttc
     3721 tctttctgcg tgacagccaa gcgccgcgaa gcctcattgc cctggtggct attgtttggg
     3781 gtgtgggcgg cgtggccatt ttgtattgga tttttaacag cctggtcgaa atcctgccgg
     3841 acgagtggac agcccggctc cagccctttg tctttgtggg gccggctatg gctattctga
     3901 cctggtatct ggccctgccg accgtgcgca ctttctggat tagcctcttt gaccgggggg
     3961 gaccgcctga tgggctttcg atccttgaac aaatgcgcag cgcttctttt gtgggcttga
     4021 gcaattatgt ggctgttttc accgaacgat tgatgataga ggccttccgc aacaatatca
     4081 tgtggattgt ctttggtagc accctctcag tgacctttgg cttgatcgtg gctgtattgg
     4141 ccgaccgcag ccgctttgag aaagtcgcca agtcgttaat atttttgcca atggccattt
     4201 cctttgtagg agccggcatc atttggaact ttatctatga gtatcgacca gcaaatcaac
     4261 cgcaagttgg cctgctaaat tctattgtcc aaggttttgg gggtcaaccg agagcttggc
     4321 aacaatgggt tgatatcatt ccgtggaaca atctctttct catcgtcatc gtcatttggc
     4381 tgcaagccgg tttcgccatg gtattgtttt ctgcggcact gaaaggcatc ccggaggagt
     4441 taatagaagc aggccgcata gatggggcta acgagttcca gatatttttc aggattatga
     4501 tcccttacat ccaggggaca cttataaccg tctggacaac catagttatc ttcaccttga
     4561 agatattcga cgtggtgtgg gtgatgactg gcgggcagtt tggcacgcac gtgattgcga
     4621 cccagtttta ccggcaatca tttacggctg ataacaaggg ctttggcgca gccattgcca
     4681 ttatcctgct catcgccgtt atcccggtga tgatttataa cctgagtcag ttcaggcaac
     4741 aggaggcgtt ctaaaatggg caaaccaacc acacaacggc gactcggtag tttgttcgtc
     4801 aacgggacat tattcctgct tgtcctcata tggactgttc ccaccctggg catttttgtc
     4861 tcttctttcc gtaaccggaa tgatatctct acctctggat ggtggaatgt gtttccgcac
     4921 cgggagtggc agccggttgc cgagattgac accaaagcca ccggacttga ccccgatggg
     4981 attatggaaa tcgagggcgc tacgggcaca ttcgaggagt ttcgggtggg catcgaaacg
     5041 ccggatggaa aacgcatcaa ctggattggc aacaagcgac ttggacgagt tgaaattcaa
     5101 gagcgggttt gggccgttaa ttggaatttc tcattggata actacaatca ggtttggggt
     5161 ggccaggatt ttgagttcac caaaccggat ggcactgtag aggtggtgcc gggtgatgat
     5221 atgtccaggg ctttcttcaa cagcctagcc gtatccattc cttctacggt gatccctatt
     5281 ctgattgctg cttttgccgc ctatgctttt gcctggatgc gttttccggg gcggcgttgg
     5341 atgtttattc tggttgtagc cttgctggtt gtccctctcc aaattgccct ggttcccatc
     5401 ttacgagatt acaaagagtt agagcttaat ggcacgtttc tggcaatctg gctggcgcat
     5461 gccggttttg ggctggcgct ggctacgtat ctgttgtaca actacatcag tgcgctgccg
     5521 cgagagatgc tggagtctgc gttcattgat ggtgcctcgc attttacggt attcttacgg
     5581 ttgatcctgc cattgtcggt cccagcgctg gcctcgttcg ccatattcca gttcctgtgg
     5641 gtatggaacg attacctggt ggcgctggtt ttcctgggtg gcaacccgga attcgagctt
     5701 gtgacacaac gtatggcggc tatcgttggt gcccgagggc aggattggca tttgttgacg
     5761 gccggagcct ttgtttcgat ggcgctgccg ttgctggtct tcttctcatt gcagcgcttc
     5821 tttgtgcgtg gcctgttagc cggttcggtg aaaggatagg catcctttac ggatttccta
     5881 agatgaccta agtcaatcca actgccaagt aatcacaaca tgcttgaact tcttgttcaa
     5941 gcaaggttat gattagttaa attttgtcgc gtcgtttata cgaacaattg atgcctgaac
     6001 agaaaatttt ctcattcgac tgggcgcttt gcttccaatg gctcatggcg actacggttg
     6061 gctgggtatt aggcaggttt ctccttccaa atctagcgtt tatcaccatt ggaatcggtc
     6121 ttggtgtcct ccaatggcta atcttacaat atcgtattaa gcacgcccag cagtggatac
     6181 ttgccacaat atttagctgg acgttaggtt cgttggtcat ccttttttta atgcccgatg
     6241 ttagcgattt catcgctggc ttggtgatcg gtgtggcaac cggaacaggg cagtggctag
     6301 tgctgcgtcg tgagctgcat tgggctggat ggtggatccc gatcaatgtc gtggcctgga
     6361 ctacaggaat ggctttgcta cctggggtga tattaaccgg tatcatggca ggtttggtaa
     6421 ccggtatagc catcgaattg ttgctccgaa cgccgcgaaa tttggtagag gtgaaataag
     6481 atccctatgc gttacagaac tttaggtaaa agcgggttga tcgtgtcaga gctggcatta
     6541 ggcacgatga tatttgg
//
LOCUS       DBNGSX010000539         6609 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_887069, whole genome shotgun sequence.
ACCESSION   DBNGSX010000539 DBNGSX010000000
VERSION     DBNGSX010000539.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6609)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6609
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_887069"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="AC16CB_15905"
     CDS             <1..689
                     /locus_tag="AC16CB_15905"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR3344007.1"
                     /translation="FNEPWGIAVGPDGAVYVADTWNHRIQKFSAEGEFIKTWGFGISQ
                     TDDPLGFYGPRGIAVDNQGHVFVTDTGNKRVVVFDTDGNYVTKFGSTGLGLGQFDEPV
                     GIAVNSAGLVYVADTWNQRIQTFIAEVNGSYLPVNSWEISGWYGQSLDNKPYIAVDNQ
                     GQIFVADPEGYRVLQFSSEGEFIQYWGDFSIELDGFDLPSAVAADTEGGIWVSDTNNN
                     RILHFTLPPK"
     gene            690..1826
                     /locus_tag="AC16CB_15910"
     CDS             690..1826
                     /locus_tag="AC16CB_15910"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3344008.1"
                     /translation="MAISIHPVISLFDQHSFYNFAWRVYRGDPNWVPHLWPQRKAYLT
                     RKAAFFNYGEGEFWLAKRGREVVGTIGTAIDHSRNRHKGQQTAIFGFFESLPGDYAIA
                     QALWDHACGWAKSRAMITLQGPYNFAANEDPGFLVEGYNCPPCIMMGHTPPDYAIYAQ
                     RYGFKKAQESLAYRYDLAQINFDVANAPEVVRRIADRAMKHHGKEVIRNPLLADWDIE
                     IERLHPVYNTSLAVLPEFSPIELVEFRAQAASLKPILDPELIFIAEVDGKSVGFALGI
                     PKINQALIHANGLRYPWDYLRYTLAQRKIHSASFKILAIDPAYWGHGLEALMFIEMAK
                     AIIRKGYTWVDASLTNEMNPQTNKLATRLGAKVYRRYREYQIPL"
     gene            2107..3264
                     /gene="sucC"
                     /locus_tag="AC16CB_15915"
     CDS             2107..3264
                     /gene="sucC"
                     /locus_tag="AC16CB_15915"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HIR3344009.1"
                     /translation="MKLHEYQSKQIFSKYGIPIPKGRIAVTAQEAEHIAEELGGRVVI
                     KSQVLVGGRGKAGGIRLAKNPDEAEELAAQILAMEIKGLPVRKILVDEAAQIEKEIYL
                     GITNDRTARKPVMMSSSAGGVEIEEIARKEPQKIIKTHIDPLLGLGDYQARDIAAGID
                     LPREHWKSFGMIAHGLWKAYLDCDATLAEINPLVITSDNRLLALDGKMVLDDNALFRH
                     PDLAEMRDLDAEAPSEIEARKYGLSFIKLDGNIGCMVNGAGLAMTSMDIIKLFGGEPA
                     NFLDIGGGASADKVTAALRIILADPNVRAVLFNVFGGITRGDEVARGILVALDEVKTK
                     VPMVIRLVGTNAEEGRQILADANMITAETLADAARKAVAAAQAQPTKGAVS"
     gene            3261..4124
                     /gene="sucD"
                     /locus_tag="AC16CB_15920"
     CDS             3261..4124
                     /gene="sucD"
                     /locus_tag="AC16CB_15920"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR3344010.1"
                     /translation="MSILVDKNTRLLVQGITGNEGLFHTQQMVEFGTNVVAGTRPGKG
                     GEWVLDGKIPVFDSVKIAVEATGANTSVIFVPARGAADAMFEATDAGLSLVVCITEGV
                     PVQDMMRVRSYMDQKGVRLVGPNCPGLLTPGATKVGIIPGDISIPGNIGVVSRSGTLT
                     YEVLFALKQLNMGVSTCVGIGGDPINGTSFIDCLDLFEEDPNTDKIVLIGEIGGSDEE
                     RAAEYIASRMTKPVVSFIAGQTAPPGKRMGHAGAIVEGGSGLASDKIRALQAAGVSVA
                     RHPEEIPDLLQ"
     gene            complement(4208..4441)
                     /locus_tag="AC16CB_15925"
     CDS             complement(4208..4441)
                     /locus_tag="AC16CB_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR3344011.1"
                     /translation="MSDTFDEIKTIIVDLLGVDGDKVTLDARFREELEADSLDLVELI
                     MAFEDKFGGEISDEDAQTITTVGEAVKYIDTHM"
     gene            4572..5603
                     /gene="fni"
                     /locus_tag="AC16CB_15930"
     CDS             4572..5603
                     /gene="fni"
                     /locus_tag="AC16CB_15930"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HIR3344012.1"
                     /translation="MTKVTQISTRKSDHIRINLEEDVRSGLTTGLERYHFIHQALPEL
                     NLEDVNLNLDIFGKKLNAPILISSMTGGTADAAAINRTLASAAQETGVAMGLGSQRAA
                     IEQPDLAVTFKVRDVAPDILLLANLGAVQLNYRYGIAECQRAVDMIEADALILHLNAL
                     QEAVQPEGDTHFAGLLKKIEHVCKSLPVPVIAKEVGWGFSEKAAKMLAEAGIAAIDVA
                     GAGGTSWSQVEMHRAQDESQSRLAAAFINWGIPTAESILNVRRAAPGLPIFASGGLRS
                     GVEIAKSIALGAHLGGMAGPFLKAAVRSLEDTLQSIAEIRNEIKVCMFAAGVPDLESL
                     RNTQLVKID"
     gene            5670..>6609
                     /locus_tag="AC16CB_15935"
     CDS             5670..>6609
                     /locus_tag="AC16CB_15935"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR3344013.1"
                     /translation="MALSNYTSIYFTPLEDELRHAVAIVNGDGLSELYEMLAYHMGWQ
                     GEGTGIGTSGKRVRPLLLLLSCEAAGGNWKDALPAAAAVELVHNFSLIHDDIEDNSPL
                     RRGRPTVWKKWGVPQALNAGDSLFTLAHLSILRLEEKVSPAITINAIRILHETCLKLT
                     QGQYMDISYEDRKDLTLEAYWPMVSGKTAALLAACTGLGALIAGADAERQDNFCQFGH
                     YLGLAFQAQDDLLGVWGNAALTGKSAESDLLTGKKSLPVLFGLSLNGPFAQRWKQGSL
                     HPDEIPLLANQLEAEGARRYTQETVDTLTDQALSHLH"
BASE COUNT         1705 a         1613 c         1700 g         1591 t
ORIGIN      
        1 tgtttaacga gccctgggga attgccgttg gtccagatgg agcggtatat gttgctgaca
       61 cctggaatca caggattcaa aaattcagcg cagaaggcga attcatcaag acttgggggt
      121 ttggtatcag ccagacagat gatcccttag gattttatgg accacgtggg attgctgtgg
      181 acaatcaggg ccatgtcttt gtcacagaca ctggcaataa gcgcgtggtc gttttcgaca
      241 ctgatggtaa ttacgtaacc aaatttggaa gtacaggttt aggccttggt caattcgacg
      301 agccggtggg cattgcggta aatagcgccg gcctagtata cgtggcggac acatggaacc
      361 agcgcattca aaccttcatc gctgaggtga atggaagcta tctaccggtc aatagctggg
      421 agattagcgg ttggtacggg caatctttgg ataataagcc ttatatcgcg gtggacaacc
      481 aggggcagat ctttgttgcc gacccagaag gctatcgggt attacagttt tcatccgaag
      541 gtgagtttat ccaatattgg ggcgatttct ccatcgaatt ggatggcttt gacttaccat
      601 cagctgtggc tgccgacacc gagggtggca tctgggtaag tgacaccaac aataacagga
      661 tattgcactt taccctgccg ccaaaataga tggcgatttc aatccatcca gtcataagcc
      721 tatttgacca gcattctttt tacaattttg cctggcgcgt ctatagagga gaccccaact
      781 gggttcctca tttatggcct caacgcaagg cctacctgac ccgtaaagct gctttcttca
      841 attatggcga aggggagttt tggttggcaa agcgcgggcg agaagtcgtc ggcaccattg
      901 gaactgccat agaccactct cgcaatcgcc ataagggaca acaaacggcg atttttggct
      961 tttttgaatc cttgcccggt gactacgcaa tcgcacaggc attgtgggat catgcctgtg
     1021 ggtgggctaa atcacgggcg atgatcacgc tccagggtcc atacaatttc gcagccaatg
     1081 aagatcctgg ctttctggtg gaaggctata attgcccgcc ctgtatcatg atgggacata
     1141 ccccacccga ttatgccatt tatgctcaac gctatggttt taagaaagct caggaaagcc
     1201 tggcttaccg ctatgattta gcccaaatta actttgatgt agccaatgcc ccagaggttg
     1261 ttcgccgcat agctgatcgc gcaatgaagc atcacgggaa agaggtgata agaaatccgc
     1321 tgttagcaga ttgggatata gaaattgaac gcctgcaccc ggtttacaac acttccctgg
     1381 cagttctgcc tgagttttca cccatcgaat tggttgaatt tcgcgcccag gctgcttcgc
     1441 ttaagccaat cttagaccct gagctaatct tcatcgcaga agtggacggt aaatcggtag
     1501 gtttcgccct aggaataccc aagatcaacc aggcgctcat ccatgccaac ggattgcgtt
     1561 atccctggga ttaccttagg tacacactcg cccagcgaaa aattcacagt gccagcttca
     1621 aaatcttggc tattgaccca gcatattggg gtcacggatt ggaggcgctt atgtttatcg
     1681 agatggcaaa ggcgattata cgcaaaggtt atacctgggt cgacgcttcc ttgaccaacg
     1741 agatgaatcc acaaaccaac aaactagcta cccgattggg agccaaggta tatcgccgct
     1801 atcgcgagta tcaaattcca ttgtgaaccg aatggatatc tttcttccgc tatggtgttg
     1861 ggggaaattc ttcagatcag caggacaatt acatatgccc ggaagggcgt gcgcaatccc
     1921 gcaataagag tgtttggggt gtattggtga gggtatgccc ccaccaaagc acccattttc
     1981 tctcctcagt ggtgggaaat cgcccaaatg cgattgccct atcagattcg ctttgcctca
     2041 acagtccaaa tggtataatg ccttcgctat catggtcaca agccaaacgc agggagagca
     2101 atttgcatga aactacatga gtaccaatca aaacagattt tttccaagta tggtatccca
     2161 atcccgaaag gtagaatagc ggtcaccgct caagaagcag agcatattgc agaagagtta
     2221 ggaggccgcg ttgtcattaa atcccaggtc ctagtcggag gtcggggcaa agctggaggc
     2281 ataagactcg ccaaaaaccc agatgaagct gaggaacttg cggcgcaaat attagcaatg
     2341 gagatcaaag gccttccagt gcgcaaaatt cttgttgatg aggctgcaca aatcgagaaa
     2401 gagatctatc taggcatcac caatgaccgt actgcccgca aacctgtgat gatgtcttct
     2461 tcggcgggag gcgtagagat cgaagagatc gcccgaaaag agccccaaaa aatcatcaag
     2521 actcacatag acccgctttt gggactagga gactatcagg cgcgtgatat cgctgccggg
     2581 atcgacctgc cgcgtgagca ctggaaatca tttggaatga ttgcccatgg tctttggaaa
     2641 gcctaccttg attgtgacgc tacgctggca gagatcaacc ccctcgtaat cacgtcagac
     2701 aaccgcttgc tggcactgga tggcaaaatg gtgcttgacg ataacgctct ctttcgtcac
     2761 cctgacctgg cggagatgcg tgacctggat gccgaggctc cctccgaaat cgaagcgcga
     2821 aagtatggcc tgtccttcat aaagttggat ggaaatatag gctgcatggt caatggtgct
     2881 ggattggcaa tgacctccat ggatattatc aagctatttg gaggagagcc agcaaacttt
     2941 cttgatattg gcggaggtgc cagcgctgac aaggtcaccg cagctttgcg catcattcta
     3001 gctgacccaa acgtgagagc ggtgcttttt aatgtcttcg gaggtatcac gcgaggcgat
     3061 gaagttgctc gaggcattct cgtcgctttg gatgaggtca aaaccaaggt ccccatggtt
     3121 attcgcttgg tgggcaccaa cgctgaagaa ggccgacaga tacttgccga tgccaatatg
     3181 atcacagccg agacgctagc tgacgctgct cgcaaagctg tcgccgccgc acaagcacaa
     3241 cctacaaaag gggcggtatc atgagcatat tagtcgacaa aaacacccgc ctgctggtgc
     3301 agggtatcac ggggaatgaa gggctttttc acacacaaca gatggtcgag tttggcacca
     3361 atgtagtagc tggaacaaga cccgggaaag gtggggaatg ggtgttagat ggaaaaatcc
     3421 cggttttcga ctcggtgaaa atcgctgtag aagccaccgg agcaaacacc agcgtgatct
     3481 tcgtcccggc ccgcggcgcg gcagacgcca tgtttgaagc tacagatgca ggtctctcat
     3541 tggtggtttg catcacagag ggcgtccctg tccaggacat gatgcgtgtg cgcagctata
     3601 tggatcagaa aggtgtgcgc ctagttggac cgaactgccc cgggctgcta acgcctggtg
     3661 caaccaaggt gggcatcatc cctggggata tatctatacc cggtaacatt ggtgtggtgt
     3721 cgcgttctgg aacgcttacc tatgaggtgc tttttgcgct caagcaatta aatatggggg
     3781 tgagcacgtg tgtgggtatt ggaggcgatc ctatcaatgg cacaagcttc atcgactgtt
     3841 tagatctttt tgaagaagac cctaatacag ataaaatcgt cctgattggg gagattggcg
     3901 gttcagatga ggaacgtgcg gctgaataca tagcaagccg tatgaccaaa cccgtggtta
     3961 gctttatcgc cgggcagaca gccccaccag gtaaacgtat ggggcatgcg ggtgccattg
     4021 tggaaggtgg atccggattg gctagcgata aaatcagagc cttgcaggct gctggcgtaa
     4081 gtgttgctcg gcatccagaa gaaataccag atttactcca ataagctttt cccccttaaa
     4141 ataatttgcc ggaaatagat atgtgcggtg caatcgcacc acagatatct atttccggca
     4201 gtttttgtta catatgtgta tcgatatatt tgaccgcctc accaacggtc gtgatcgttt
     4261 gggcgtcttc gtcagaaatc tctcctccaa atttatcttc aaaggccata attagctcca
     4321 caagatcgag cgaatcagcc tcgagctcct cacgaaagcg cgcatctaaa gtgactttat
     4381 caccatcaac cccaagcagg tcgacgataa tggttttgat ttcatcaaat gtatcggaca
     4441 tatagcatgc ctccttcggt taattataat cctgccaatt ttactcaggg tatctaatct
     4501 gtcaagccca gtatgttgac aggcaaaaat cagactggta agatagctct aactgacagg
     4561 aacaccttta catgacaaaa gttacgcaga ttagcaccag aaaatccgat catattcgca
     4621 ttaatctgga ggaagatgta cgttccggtc tcaccacggg gctggagcgt tatcatttta
     4681 tccaccaggc actacctgaa ctaaatcttg aagatgtgaa tttaaatctc gacatctttg
     4741 gaaaaaaact caacgccccg atcctaattt cctccatgac aggtggcacc gctgatgcag
     4801 cagccatcaa ccgtacgctg gcctcagcgg cccaggagac aggcgtggcc atggggctag
     4861 gctcccaaag agcagctatt gagcaaccgg atttagccgt caccttcaaa gtgcgagatg
     4921 tcgcccccga tattttgctg cttgctaacc ttggagccgt acaacttaac taccgttatg
     4981 ggatagccga atgtcagcgt gctgtggata tgatcgaggc agatgcattg attttgcacc
     5041 taaatgccct tcaagaggct gtccagcccg aaggagacac gcactttgcc gggctcttaa
     5101 aaaaaattga acatgtgtgt aaatccctcc ctgtgcctgt catcgccaaa gaggtcggtt
     5161 ggggattctc tgagaaagct gccaaaatgc tagccgaggc tggcattgct gccatcgatg
     5221 ttgccggggc tggtggcaca tcctggtcgc aggttgaaat gcaccgcgcc caagacgaaa
     5281 gtcaatccag acttgcagca gcattcataa attggggcat acctaccgcc gaatcaattc
     5341 ttaatgtacg gcgcgctgca cctggtttgc caatttttgc ctcgggtggc ctgcgcagcg
     5401 gggtagaaat cgccaaatcc atagcccttg gagcccactt aggtggcatg gctggaccct
     5461 tcctcaaagc cgctgttcgt tccctggaag atacactcca gagcatcgct gaaatccgaa
     5521 atgaaatcaa ggtgtgtatg tttgcagcag gagtgccaga tctagaaagc ttgcgcaata
     5581 ctcagcttgt caaaatcgat tgacatttta cccgaagtac ccttttcgga acggaatgga
     5641 acgaaaaaga tttttattct aggaatacta tggcgctgtc aaattacact tcgatatatt
     5701 ttacgccctt agaagacgaa cttcgccatg ccgtagcgat tgtgaatggc gacgggttgt
     5761 cagagctgta tgaaatgctc gcctaccaca tgggctggca aggggagggc actggtattg
     5821 gaacatcggg taaaagggtt cgcccattac tgttgctgtt atcgtgcgaa gctgctggcg
     5881 gaaattggaa agacgccctt ccggccgcgg ctgctgtaga gctagtgcac aacttctcgc
     5941 tcatccacga cgatattgaa gataacagcc cattacgacg cggccgccct actgtctgga
     6001 aaaaatgggg cgtaccgcag gctcttaacg ccggcgattc gctcttcacc ttggcgcact
     6061 tgtcgattct gcgattggaa gagaaagtgt cccctgccat caccatcaat gcaatccgca
     6121 ttttacatga aacatgcctc aagctcaccc aggggcaata tatggatatc tcctacgaag
     6181 atcggaaaga cctaacctta gaagcatact ggccaatggt gagcggtaaa accgctgcgt
     6241 tactggccgc ttgcaccggg ttgggcgcgc tcattgcagg agcagatgct gagaggcagg
     6301 ataatttttg ccaatttggt cattacttgg gattggcatt ccaagctcaa gatgatttgc
     6361 tgggtgtgtg gggtaatgca gctctgacag ggaaatctgc tgagagcgat ctgcttaccg
     6421 gtaaaaaatc tcttcccgtg ctctttggcc tcagcctaaa cggtcccttt gcgcagcgct
     6481 ggaagcaagg gtcattgcat cccgatgaaa tccccctcct ggcaaatcag ttagaagccg
     6541 aaggcgcccg tcgctatact caagaaactg ttgacacgct caccgaccaa gctttgagtc
     6601 atctacaca
//
LOCUS       DBNGSX010000540         4693 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1370181, whole genome shotgun sequence.
ACCESSION   DBNGSX010000540 DBNGSX010000000
VERSION     DBNGSX010000540.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1370181"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="AC16CB_15940"
     CDS             complement(<1..275)
                     /locus_tag="AC16CB_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344014.1"
                     /translation="MEKEEQITRNNFISMVIWAVGGLISLGMGIPAIAYIIGPALSRN
                     QVQEWIRLGPISKIELNIPTLFKFVIQRKTGWIVNEEEISVYVLTDNG"
     gene            complement(298..1923)
                     /locus_tag="AC16CB_15945"
     CDS             complement(298..1923)
                     /locus_tag="AC16CB_15945"
                     /inference="COORDINATES: protein motif:HMM:NF015237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HIR3344015.1"
                     /translation="MPYALLGVLTLMLLTAAAYAWDYTNSPGFCGQACHTMPPEYTAY
                     LLSPHARVDCVDCHIGKGFIATRISRKASDLRHVYATAFVSYEFPIRADDLRPARETC
                     ELCHSPQKFSDDSLREIKRFGDDRANTPISIYLVLKTGGGTRREGLGRGIHWHIENKI
                     DFLATDGHEQEIPYVRVIEDDGSITEYMDLETNIVPSSIKDSDLKRMDCISCHNRITH
                     MVLSPVDTVDKLMARGLIASDIPEIHQKAVNVYSTVFQNTEVGLNRIASLREFYKTYY
                     PDYYAENQEKVEAAISALQQAYSNSVYPEQSSDWTTHPSNVGHQNSPGCFRCHDGKHL
                     NQQGEAIRLECNLCHSIPVVAGPNEFVADIEISRGPEPETHLNPNWISQHRDVFDPTC
                     SNCHTTDNPGGTDNTSFCSNSACHGSAWEYAGFDAPGLREIILAQLPPAPTPVPLPSG
                     ESTTYEATIGPLFEMRCGSCHGATSSIQGLNLGIYEDAMKGGDSGSVIVPGDPQGSLL
                     IQKQSGEQAHFGQFTSEELDLIVQWIQDGAPEN"
     gene            complement(2092..3339)
                     /locus_tag="AC16CB_15950"
     CDS             complement(2092..3339)
                     /locus_tag="AC16CB_15950"
                     /inference="COORDINATES: protein motif:HMM:NF024827.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HIR3344016.1"
                     /translation="MKHRWILNLGVLLLALVLLAACASPQGPAGDSGPPGPPGPEGPQ
                     GPAGEQGPAGPEGKVGEAAAEYIGAATCGGCHQELYDAFMKSGHAWKLNPVVDGQPPE
                     YPFTEVDQLPEGYTWDDISYVIGGYNWKARFIDQNGYIITDKPGAVISDTTYLNQWNF
                     ANPMVGKEAGWVTYNSGAKELEYTCGNCHTTGYSPGGNQDGKPGLVGTWEEPGIKCEE
                     CHGPGSLHASNPRGFAMQIDRDAELCGQCHIRNAIEAVDAEDWFINHHEQYEELFQSK
                     HVTIDCVVCHDPHKLVVQLRQSGEATTRTQCQNCHFKQAQFQNNSKHVAMNIACIECH
                     MPRIVKSAWGDPATFTGDIRTHLMAIDPNQIDQFSKDGTTALSQISLDFACRHCHVEG
                     RGFPKTDEELKAVANGYHNPPEP"
     gene            3624..>4693
                     /locus_tag="AC16CB_15955"
     CDS             3624..>4693
                     /locus_tag="AC16CB_15955"
                     /inference="COORDINATES: protein motif:HMM:NF019350.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HIR3344017.1"
                     /translation="MNAPELQFDIVVVFFIYGLAFFSMGLTVMMEARRSPMLAEARVL
                     WPLAFFGFVHGSHEWLEIGLDVRRWFGFNDPLYIPWIRLVLLVISFASLVLYSLQVLR
                     PQGRVLSQSNFQTVLALLGLYLILVIAIGLIQPNSLEVLQRRADALARYALAVPGAGL
                     AALALIRQASQVPTTERRELTIGLHLAALGFAIYSLTQIMVTPVDIFPARYLNSTAFL
                     NWAGFPIQAVRACMAVLVTVGLIQATQVVEGDRQRQLASAQEARLRALEQIRLELAER
                     EALRRELLRHTVAAQEEERSRIARELHDETAQFLTALSLDLATLRKSLHRNKEAVHIL
                     DRLQDMRSKMSQGIYRMVHDLRP"
BASE COUNT         1027 a         1246 c         1228 g         1192 t
ORIGIN      
        1 ccgttgtcgg ttaacacgta aaccgaaatt tcctcctcat tcacaatcca accagttttg
       61 cgctggatca cgaatttaaa taaggtaggg atgtttagct cgatttttga tatcggtcca
      121 agccgaatcc attcttgtac ttggttgcga ctgagggctg gaccgataat atacgcgatg
      181 gcaggtattc ccatgccaag gctgattaat cctccaacag cccaaatcac catgctgata
      241 aagttattgc gagtaatttg ctcctctttt tccatttctc catccaataa ggttaattta
      301 gttttccggc gcgccgtctt gaatccactg aacgatcaaa tccagctctt cggatgtaaa
      361 ctggccaaag tgtgcttgct caccggattg tttttgtatt agcaggctgc cctgcgggtc
      421 tcccggaacg atgacagagc cactgtcgcc gcccttcatt gcgtcttcat atatgcccaa
      481 gttcaggcct tggatactgc tggtggcgcc atggcaactg ccacagcgca tttcaaagag
      541 cggaccgatt gtggcctcat aagtggttga ctcaccagaa ggcaagggta ccggtgtggg
      601 ggcagggggg agttgtgcca ggatgatttc ccgcaatcca ggcgcatcga aacctgcata
      661 ttcccaggca ctgccgtggc aggcactgtt cgagcaaaac gacgtgttgt ctgtgccacc
      721 cggattgtct gtcgtgtggc agttagaaca tgtggggtca aacacatccc ggtgctggga
      781 aatccagttt gggttcaggt gcgtttctgg ctcgggtcca cggctgatct cgatatcggc
      841 cacaaactca ttcgggcctg ccaccacagg tatcgagtga cacaagttgc actccaagcg
      901 gatagcttcg ccttgctggt tgaggtgttt gccatcatga cagcggaagc aaccaggtga
      961 gttctgatga cccacattgc ttgggtgggt agtccagtcc gaactttgct ccggatatac
     1021 gctattgcta tatgcctgct gcaaagccga aatagctgcc tcgaccttct cctgattctc
     1081 agcgtaatag tccggataat aggtcttata aaactccctc aggctcgcga tacggttcaa
     1141 gcccacctcg gtattttgga aaaccgtgct atagacattc actgcctttt gatggatctc
     1201 ggggatgtcc gacgcgatta agccgcgtgc catcaacttg tctactgtat ctacagggga
     1261 taacaccata tgggtgattc ggttatggca actgatgcag tccatacgct tcaggtcgct
     1321 gtctttgatc gacgacggga caatattggt ctctagatcc atgtattctg tgatcgaacc
     1381 gtcatcttcg atcacgcgca catagggaat ctcctgctca tgaccatctg tcgccagaaa
     1441 atcgatcttg ttttcaatat gccagtggat gccgcgcccc aacccctctc gccgggtgcc
     1501 gccgccggtc tttagcacca ggtaaatgct gatgggtgtg ttggcgcgat cgtcgccaaa
     1561 gcgtttgatc tctcgcaggc tgtcatctga gaatttctgt ggagaatggc acagctcaca
     1621 ggtctcgcgc gccgggcgca agtcatcggc gcggatcggg aactcatacg atacgaacgc
     1681 cgtagcatac acgtgcctga gatcactggc ctttcgagaa atacgcgtgg cgataaatcc
     1741 ctttccgata tggcaatcca cgcagtcgac acgggcatgc ggcgaaagca aataggctgt
     1801 atactctggc ggcatggtat ggcaagcctg gccacagaac ccaggagagt tcgtataatc
     1861 ccaggcatag gcggcagcgg tgagcagcat cagtgtcagc acacccaaca aagcgtaggg
     1921 cataagccgc acccatcgag atgcgtcaac aggagggaaa aagaagcgtt taagactttg
     1981 aattattttt ttcattcgta tatataccat ttgtgtgatg gaaggtcatc acccatccat
     2041 cacactgaag aagatcatct ccttccaaaa ggcaggaaaa tttgtgaata gttaaggctc
     2101 cggcggatta tgatagccgt ttgcaacagc cttcaactct tcatcggtct tggggaaacc
     2161 tctgccttcc acatggcagt ggcggcaagc aaaatccagg ctgatttgcg agagagctgt
     2221 tgtgccgtct ttgctgaatt ggtcaatctg attcgggtca atcgccataa gatgggtgcg
     2281 aatgtcacca gtaaacgtgg cagggtcacc ccaggcagat ttgacaatgc gcggcatatg
     2341 gcactcgata caggctatgt tcatggcgac gtgcttgcta ttgttctgga attgggcctg
     2401 cttgaagtgg caattctggc actgggtgcg cgtggtcgct tcgccgcttt gtcgcaattg
     2461 caccactagc ttgtgcgggt cgtgacacac tacgcagtcg atggtcacat gtttgctctg
     2521 gaatagctct tcgtactgct cgtgatggtt tatgaaccag tcttccgcat ccactgcctc
     2581 gatggcattg cgaatgtggc actgcccgca aagctcggca tcgcgatcga tctgcatggc
     2641 aaaaccgcgc gggttcgagg cgtgcaagct gcccgggcca tgacactcct cgcatttaat
     2701 gccaggttct tcccatgttc ccaccagacc aggcttgcca tcctggttgc caccagggct
     2761 gtaacccgtg gtatggcagt tgccacaggt atactctaac tctttcgcgc cggagttata
     2821 tgtcacccat ccggcttctt tgcctaccat gggattggcg aaattccact ggttgaggta
     2881 cgtagtgtcg ctaattaccg cgcctggctt atcagtgatg atgtagccat tttgatcgat
     2941 gaagcgggct ttccagttat agccaccgat tacatagctg atgtcatccc aggtatatcc
     3001 ttcggggagc tggtctactt cggtaaacgg atattctggc ggctggccat ccaccaccgg
     3061 gttgagcttc caggcatgcc cagacttcat aaaggcatcg tatagctcct ggtggcatcc
     3121 cccacaggtt gcggctccaa tgtattctgc ggcagcttct cctactttgc cttcagggcc
     3181 ggcgggacct tgctcgccgg caggtccttg agggccctca gggccaggag gaccaggagg
     3241 accggaatcg ccagctggac cttgtggact ggcgcaggca gccaacaaga ccaacgctag
     3301 caacaaaacg cccaaattca atatccaacg atgcttcata atagatgtat tctcctcatt
     3361 gctgggcaaa atgatgatct agagttggga atcaccacac atttgcggtt gaattttgcc
     3421 cttccaggat atataatgta cttgggattg ggttatacac ccaatatacg ctcgtcttgt
     3481 tgggtcactc acaccacaca gtatacctta agtcaagatg agagactagt gaaatttttc
     3541 tcatagcttg ggttattagt catatatatt agttgattaa catcctctga tttgtggaaa
     3601 aacgggtgag ctggcataac cctatgaatg cgccagagtt acaattcgat atcgttgtcg
     3661 tcttcttcat ctatggattg gcctttttta gcatggggct aaccgtgatg atggaggcca
     3721 ggcgttctcc aatgctggct gaggcgcgcg tgctgtggcc gctggcgttc tttggatttg
     3781 tgcacggttc gcacgaatgg ctggagatcg gattggatgt gcgccgctgg ttcggtttta
     3841 acgacccact ttatatcccc tggatccggc tggtgctctt ggtaatatcc tttgcttcgc
     3901 tggttctcta cagcctgcag gtgttgcgcc cccagggcag ggttttgtcg cagtcgaatt
     3961 ttcagaccgt gcttgccttg cttgggcttt acctgatttt ggtcatcgcc attggattga
     4021 tccagcccaa ttccttagag gtgttacaga ggcgcgccga tgccttggca cggtacgccc
     4081 tagctgtacc tggggcaggc ttagccgctc tggcgcttat ccgccaagcc agtcaggtgc
     4141 caaccaccga acgccgtgag ttgaccatcg gcttacacct agccgccctg ggttttgcca
     4201 tttacagcct gacccaaata atggtcaccc cggtagatat cttccctgcc cgatatctga
     4261 actcgaccgc ctttctgaac tgggcaggct ttcccatcca ggcggtgcgc gcatgtatgg
     4321 cggttctggt caccgtcggc ctgatccagg ctacccaggt ggtggaagga gatcgccaaa
     4381 gacaattggc ctctgcccag gaagcacgcc ttcgtgcctt ggaacaaatt cgtcttgagc
     4441 tggctgaacg cgaagctttg cgccgcgagc tcttgcgaca cactgtggct gctcaagagg
     4501 aagaacgctc ccgcatcgct cgtgaattgc acgatgagac tgctcaattc ctgaccgctc
     4561 tcagcctgga tctggcaaca ctccgcaaat ccctgcaccg caacaaggaa gcagtgcaca
     4621 tcctggacag gctgcaagac atgagaagca agatgtcaca gggcatttac cgcatggtgc
     4681 acgatctgcg ccc
//
LOCUS       DBNGSX010000541         3146 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1786228, whole genome shotgun sequence.
ACCESSION   DBNGSX010000541 DBNGSX010000000
VERSION     DBNGSX010000541.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1786228"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(467..1972)
                     /locus_tag="AC16CB_15960"
     CDS             complement(467..1972)
                     /locus_tag="AC16CB_15960"
                     /inference="COORDINATES: protein motif:HMM:NF014051.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR3344018.1"
                     /translation="MMSDDQFVRRMLKKFSGLSKDMLVYGAGGALIQLFGLITVPIVT
                     RLFSPEEYGTIDLIYQSMGYLSVLLGMNVTSGLLRYFYEASTKDPSEQQRMVSTTFWS
                     AAGPSFLAAAILSAFARPFSQWLFNTSEPASALQLAAYTVPLQLLFGLLTALQRLECR
                     PSTYIALNIGYALINYALVITFVAVLRVGLIGIFIAQICAYTVGVLAAMLLANRLIGF
                     AFSWHWMRKVYGYALPMVPTALMTWWLATSNRFFLNTYRGLAEVGYYAVASKIAMAMQ
                     FIVQTFILAWEPFMLENLKNPEAPRIYAVALKYYSLGMLAIGAIISAFARDLFLIMAP
                     VSYLPGTALVAFIVLRYILNGAGYITGVGVVAREMSYYYSVALFCGLIANLASNLLLT
                     PRYGIYGAAISEATGIVVSTLVTSYISWRLMPIRWKLVPPALASLGFVLTSIITNNLS
                     SLAESWNMIWLLLVKLALLVVCFSAMALLIERKYLDLMFVELPRRLARRIM"
     gene            complement(1999..2952)
                     /locus_tag="AC16CB_15965"
     CDS             complement(1999..2952)
                     /locus_tag="AC16CB_15965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3344019.1"
                     /translation="MTHPSNYTISVVIPAYNAERWIGEALDSVLAQTYPPTEVIVVDD
                     GSTDGTRSVIQRYAGRVNYLYQENRGQAAARNAGIRAAIGEYIALIDADDLWQAEKLA
                     LQVEKLKEGYEWVICNFANFDDSTHQLVDMPKLKLYEGDVLEQLFLICFIGSPTPVVK
                     RDIFDKVGGFDESPSLLFGEDWEMWLRIAASYPLGVVHKTLALHRLHPTSAMAITDPM
                     QKMENKKKIIEEIVARYPARLGKLKFRALSHWSYGLGVTLFKQEQYFEAKAQFLKVLE
                     HRPFHGGALTYLTMIALGPGFSKPIVRWKRRLFAWLKRLET"
BASE COUNT          888 a          757 c          727 g          774 t
ORIGIN      
        1 aaatagatcc acaccaaagg cgttcagcca tcggaaaatc cttacctcca agccctcagg
       61 aatgagcctt agtaatcccc tcttggtcaa taaagtcccc aataaaaccc tggaatggta
      121 ctgctctgtg tccgttcttg ttgatttata accaatcaag cacctccgga tgaaaggcaa
      181 gatttactca tcgtcattta gccaaccatt attcaataaa gctagttttg tattcacgat
      241 aggggcattt tttcgcacta aattcactat tataattcgc tctgaagtac gaaaaagttt
      301 gttgttcgaa actgttcatg tgtttcttgg taattgaata tttgatataa ataccctgat
      361 ggtgttattc tgaacatctt attcatacca ttctctataa aaaaataata gaagtcttgt
      421 agaaatggaa gtaaatttcg ccttattatc ttttttaaca agatctttac ataatccttc
      481 gagccaatcg tcggggtaat tcgacaaaca ttaggtcaag gtacttgcgc tcgatcaata
      541 gagccatagc actaaaacac acaactagga gcgccaattt taccagcaat agccaaatca
      601 tattccagga ttctgccaaa gaagaaaggt tattggtgat gatcgaggtc agtacaaaac
      661 cgagcgaggc caaagcgggg ggcacaagct tccaacggat cggcatcaag cgccaggaga
      721 tatagctggt aaccagggtg ctcacaacaa tccccgtcgc ctctgaaatg gcggcaccgt
      781 aaatgccata gcgtggggtt agcagcaaat tcgatgcgag gttagctatc aaaccacaaa
      841 aaagagcaac agaataataa tagctcattt ctcgggctac tacgccaacg cctgtgatgt
      901 atcctgcccc gttgagaatg taacgcagga caataaaggc caccagagct gttccgggca
      961 ggtaggatac aggtgccatg atgaggaata gatcacgtgc aaaagcggaa atgatcgcgc
     1021 caatcgccaa cattcctagc gaatagtatt tcaacgccac cgcataaatt cgaggcgctt
     1081 ctggattttt gagattctcc agcatgaatg gttcccatgc caggataaac gtctgtacaa
     1141 tgaactgcat cgccatggcg atcttactgg ctacagcgta ataacccact tccgctaaac
     1201 cgcgataagt gtttaggaaa aaccggttcg aagtggccag ccaccaggtc atgagcgcag
     1261 tgggtaccat aggcagagca taaccgtaca ctttgcgcat ccaatgccag gagaacgcga
     1321 atccgatcag ccggtttgcc agtagcatag ctgcgagcac cccaaccgtg taggcacaga
     1381 tttgggctat gaatataccg atcaaaccca ctcgcaggac cgcgacgaac gtaataacga
     1441 gtgcataatt aattagggcg tatccaatat ttaatgcaat ataggtagaa ggacggcatt
     1501 ctaaacgctg gagcgcagtc agcaacccga atagcagttg aaggggcacc gtataagcag
     1561 ctaattgtag ggctgaagca ggctccgagg tattaaacag ccactgggag aatggtctgg
     1621 caaaggcgga tagaatcgca gcagccagga acgaaggccc agcggcactc caaaaagtcg
     1681 ttgaaaccat ccgctgctgc tcactgggat cctttgttga ggcttcgtaa aaatatcgga
     1741 gcaggcccga ggtaacgttc atccccagta gaacggataa ataacccatg gactggtaga
     1801 tcaagtcaat agtgccatat tcctctggtg aaaaaagccg cgtgactatc ggtactgtga
     1861 tcaatccaaa gagttggatt aaggccccac ctgccccata taccagcata tctttcgaaa
     1921 ggccgctgaa ttttttcagc attcgcctaa cgaattggtc gtcactcatc atccgatttt
     1981 catcctttgg gagatcgatc acgtctctaa cctcttcagc caggcaaaaa gacgtcgttt
     2041 ccaacggaca atcggtttgg aaaagcctgg tcccaacgca atcatcgtca ggtaagtcag
     2101 ggcgcctcca tgaaaaggac ggtgttcgag taccttcagg aattgggctt tggcttcgaa
     2161 atattgctct tgtttaaata gagtcacgcc taagccatag ctccagtgcg acaaggcgcg
     2221 aaatttcaat tttcccagtc gagctggata gcgcgcaaca atctcctcga tgatcttctt
     2281 tttattttcc atcttctgca tgggatcggt gatagccatg gcgctggttg gatggagacg
     2341 atgtaaggcc agcgttttgt gcactactcc taaggggtag ctggcggcaa tccgcagcca
     2401 catttcccaa tcttctccaa aaagcaatga tggggattcg tcaaatccac caaccttatc
     2461 gaaaatgtcg cgctttacga ctggcgtggg cgagccaatg aaacagatta aaaatagctg
     2521 ctccagaaca tcgccttcat atagtttgag tttaggcata tccacgagct ggtgagtgct
     2581 gtcatcaaag ttggcaaagt tacagattac ccattcataa ccctctttga gcttttcgac
     2641 ctgtagagcc agtttctccg cttgccataa atcatcggca tcgattaagg cgatgtattc
     2701 tccaattgct gcgcggatgc cagcattgcg agctgcggct tgtccacgat tttcttgata
     2761 gagataattg acgcgaccag catatcgctg gataaccgaa cgcgtcccat ccgtggagcc
     2821 atcatccacc acaatcacct cagttggcgg gtaagtttga gccagaacac tgtccaatgc
     2881 ctccccgatc cagcgctctg cattataggc gggtatcacc acagatatgg tgtaattcga
     2941 agggtgagtc atcttcgatc cttcattatg agttaatcag gctgagattt agtcccgacc
     3001 ggaaaacgtt ttgctctcca aacttggacc aattgatgcc tccatcggct gttaagcaac
     3061 gacgctgcga agccaaggta ggcgtgtggt tgaaatggcc agtgggctat ggattgtgct
     3121 gtgtgctggc gaacaagtct catttg
//
LOCUS       DBNGSX010000542         2809 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1302279, whole genome shotgun sequence.
ACCESSION   DBNGSX010000542 DBNGSX010000000
VERSION     DBNGSX010000542.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1302279"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..1601)
                     /gene="gatB"
                     /locus_tag="AC16CB_15970"
     CDS             complement(168..1601)
                     /gene="gatB"
                     /locus_tag="AC16CB_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR3344020.1"
                     /translation="MNEYEAVIGLETHIQLNTLTKIFCACKADSWNDPPNTNICPVCT
                     GLPGVLPVLNKAVVEKAILLAAAMQAEIQPLSYFDRKNYFYPDLPKGYQISQFDIPLA
                     RGGYLDLPMPEGYTRRVNIHKLHIEEDAGKTKNDDGRRQIDFNRCGVPLVEMVTAPDL
                     RSADEAAQYLIRLRQLLRWLGVSEADMEKAHLRCDANVSIRPKGTDILNPKTEIKNVN
                     SIVAVRQAIQTEVERQIREVGAGKRVEAWTLEWDEDAGVLRKMRSKETAADYRYFHEP
                     DLLPLVIDEDWKDEILANLPELPLERRNRFKQQYGLPEYDADILTGERSLSEYFEAAV
                     TSYGGDPKRVSNWLMNDVLRMINESGVPAGQLRLTPEYLAGIIKMVDEGTLNANTGKS
                     LLDKVEKTGRSPDEIVEAEGLAQVSDTAAIRGVCAEVIAENPEQVANYKAGKVTLMGW
                     FVGQVMKKSRGKADPQVARAMLEELLA"
     gene            complement(1616..2299)
                     /locus_tag="AC16CB_15975"
     CDS             complement(1616..2299)
                     /locus_tag="AC16CB_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HIR3344021.1"
                     /translation="MSKRLSSEEVLARLHSLANPANVAGMARYGINTHNTLGISIYTL
                     RPLAKEIGRDHTLSLELWDSGIHEARILASYIDDPEQVTEEQLEDWVVDFDSWDVCDQ
                     VCNLFAKTPWAYQKAFQWSKREEEFVKRAGFVLMAVLSVHDKQAPDEKLAQFFPVIMR
                     ESTDERNFVKKAVNWALRNIGKRNRHLNELAIETAQQIREIGAKSARWIASDALRELT
                     GEKVQARLR"
     gene            complement(2388..>2809)
                     /locus_tag="AC16CB_15980"
     CDS             complement(2388..>2809)
                     /locus_tag="AC16CB_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR3344022.1"
                     /translation="LMGMYVSAAQYSEQYYNRALRVRTLIRSDFDQAFDSQGPYRLQA
                     LLTPTTPTTAFPLGAVYGDSVLMQYADQLTVPANHAGVPGLSLPAGFDRDGLPIGIQL
                     LGPDFSEETLLRIGRAYEMATETDEWRGKRPPVLSKI"
BASE COUNT          634 a          793 c          735 g          647 t
ORIGIN      
        1 aggtgctact agttcccaaa tgcgagcgtt tgaggccagt ccatgcagca acaccataaa
       61 ccgcttgcca tccgccaaat tccagtggag ataatgcaag cgcagttcat taaggaataa
      121 gtaagacgat ccagccacag ggaacatgaa tgagttgacg gggcagccta agccaacaat
      181 tcttccagca tcgccctggc tacctgcgga tcggctttgc cgcgggattt cttcatcacc
      241 tgcccaacga accagcccat caaggtaact ttacccgcct tataatttgc cacttgctcg
      301 ggattctcgg cgatcacttc agcgcacacc ccgcgaatgg cagcagtatc gctcacctgg
      361 gccaacccct ccgcttcgac aatttcgtcg ggagagcgac ccgttttttc tactttatcc
      421 agcagagatt tacccgtgtt ggcgttgagc gtgccctcat ccaccatttt gatgatcccc
      481 gccagatatt caggtgtcag gcgaagttgg ccagcaggca caccgctctc gttgatcatg
      541 cgcagcacgt cgttcatcag ccagttcgag acgcgcttgg ggtcaccacc gtatgacgtt
      601 accgctgcct caaaatactc agacaggctg cgctccccag tcagaatatc tgcatcgtat
      661 tcaggtaagc cgtactgctg tttgaagcgg ttcctccgtt ccaggggtaa ttcaggcaga
      721 tttgccagga tttcatcttt ccaatcttca tcgatgacca atggcagcaa gtccggctcg
      781 tggaaatagc ggtaatcggc agccgtctcc ttagagcgca tcttgcgcag aacaccagcg
      841 tcctcatccc actctagtgt ccaggcttcg acgcgcttgc cggcccccac ctcgcgaatc
      901 tggcgctcga cctcggtctg gatagcctgg cgcaccgcca cgatagagtt gacgttcttg
      961 atttcggtct tagggttgag tatatcagtg cctttcgggc gaatagagac gttggcgtcg
     1021 cagcgcagat gagccttttc catgtcggct tcggagacgc ccagccagcg tagcaactgg
     1081 cgcaaacgaa tcagatattg ggcggcctca tcagccgagc gtaggtcggg ggcagtgacc
     1141 atttcgacca gcggtactcc acagcggttg aagtcaatct gccgtcgccc atcatcgttc
     1201 ttagtcttgc cagcgtcctc ctcgatatgc agcttatgga tattcacgcg gcgggtgtag
     1261 ccctccggca tgggcaagtc caggtaaccg ccgcgcgcca gagggatatc aaattgcgat
     1321 atctggtagc ccttgggaag atcggggtag aaataatttt tgcgatcaaa atacgataga
     1381 ggctgaattt cagcctgcat ggcggcagcc agcaagatag ccttctccac aacggctttg
     1441 ttcaacactg gcagtacccc tggcaatcct gtacacaccg gacagatatt ggtattaggc
     1501 gggtcgttcc aggaatcagc cttgcaggcg cagaagatct tggtcagcgt attaagttgg
     1561 atatgagttt ctaacccaat gacggcttca tactcattca tgaaatgaac cctcgttagc
     1621 gcaacctggc ctggactttc tcaccggtca gctcacgcaa tgcgtcggag gcgatccagc
     1681 gggcagattt ggcaccgatc tcacggattt gctgggcggt ctcgatcgcc agctcgttta
     1741 gatggcgatt acgcttgccg atattacgca atgcccagtt gaccgctttc ttgacaaagt
     1801 tgcgctcgtc agttgattca cgcatgatga caggaaagaa ctgggctagc ttctcatcgg
     1861 gggcttgttt atcgtgcaca gataacacag ccatcaggac aaatccagcg cgcttgacaa
     1921 actcctcttc acgcttgctc cattggaagg ctttctgata agcccacggt gttttcgcaa
     1981 acaggttaca cacctggtcg cacacgtccc aggagtcaaa atcgaccacc caatcttcca
     2041 attgctcctc ggtgacctgc tcgggatcgt caatgtaact ggccaggatg cgcgcctcgt
     2101 gaatacccga atcccataat tcaagggaaa gggtgtggtc acgcccgatc tctttggcga
     2161 gtggtcgcaa ggtgtagata gaaatgccca gagtgttatg ggtattgatg ccatagcgcg
     2221 ccatgccagc cacgttggcg gggttggcca aggaatgtag acgggcgagg acttcttcgc
     2281 tggataacct tttactcatt ttgcgcctaa atcaagcacc aagggcacta atttctttat
     2341 cttccttcac gtcttgtgtg ttagaaatta ctccgaactt gccagaatca gatcttactc
     2401 agtacaggcg gtctcttacc ccgccactcg tcggtctcag tcgccatctc gtaagcacgg
     2461 ccaatccgca atagggtctc ctcagagaaa tctggaccca ggagctgaat gccaatcggc
     2521 aagccgtccc ggtcaaagcc cgccggtaga gataagccag gtaccccggc atggttggca
     2581 ggcacagtta actgatcggc atactgcatc agcaccgagt cgccgtacac cgcgcccaga
     2641 gggaaagctg tggtcggcgt agtgggggta agcaaggctt gcaaacggta gggtccctgg
     2701 gaatcgaagg cttgatcgaa gtcgctacgg atgagggtgc gcacacgcag agcccggttg
     2761 tagtactgct cgctgtactg ggcagcactg acatacatgc ccatcagga
//
LOCUS       DBNGSX010000543         5377 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_124509, whole genome shotgun sequence.
ACCESSION   DBNGSX010000543 DBNGSX010000000
VERSION     DBNGSX010000543.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_124509"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1315
                     /locus_tag="AC16CB_15985"
     CDS             116..1315
                     /locus_tag="AC16CB_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017516924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3344023.1"
                     /translation="MKANKILFRWLVLLVSLSLVLTACGGGAVTPAPEAEGGPLQSIG
                     EGEGAVSIIAWAGYIERGETDPNFDWVTQFEKDTGCMVSVKVAATSDEMVALMNEGGF
                     DLVTASGDASNRLISSGRVQEINIDLIPSWSTVDERLQNAPWNTVDGKHYGTPYQWGP
                     NVLMYNTDVFPTPPDSWSVVFEEQTLPDGQSNKGRVQAYDGPIYIADAALYLMVNNPE
                     LGIKDPYNLDRDQFNASLDLLRQQRQIVGRYWHDAFVQMDDFTNEGFVASSSWPFQVN
                     LLQFEDKPIGSVFPKEGATGWSDTTMMHVDAPHPNCAYKWLEHSLNPKLQGDLAAWFG
                     SVPAVPAACTGNELLTDEGCDINGMKDFDKIHFWKTPTTDCGDGRQECVPYHEWVTNY
                     IAVIGGR"
     gene            1376..2416
                     /locus_tag="AC16CB_15990"
     CDS             1376..2416
                     /locus_tag="AC16CB_15990"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018556753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3344024.1"
                     /translation="MNDTAVPAVRLSNVSRLFGEFAAVDDVSLDIQDGEFFTMLGPSG
                     SGKTTCLRLVAGFDRPTYGTILLHGKDVTQLPPYEREVNTVFQDYALFPHMSVGDNVG
                     YGLMIDKVPKAKRQERVEEMLELVQLSGLTGRKPSQLSGGQRQRVALARALIKHPRVL
                     LLDEPLGALDLKLRQQMQIELKAIQQRVGITFVYVTHDQEEALTMSDRIAVFNRGKSE
                     QVGTPAEIYEHPATPFVAGFVGVSNLVSGEAAQKITGSADTFSIRPEKIRLAEPDLDV
                     PANMYSADGAVRDVIYLGLYTRYLIDLELGEDLVVIQQNLDATSMDVLAARGKKVRLL
                     WLRDHNRALKSA"
     gene            2460..3401
                     /locus_tag="AC16CB_15995"
     CDS             2460..3401
                     /locus_tag="AC16CB_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3344025.1"
                     /translation="MSVSPGKPGRSSLSTRVSSYLYKRPTAILLLLLGPPMAWMFIIY
                     LGSLFALLVNSFFSLDGYTGLVVRQFTLNTYRDLFTRANFDIVQRTAGMAAAVTVTAA
                     ILAFPLALFIARYASRRVKGILYLAVLLPLWSSYLVRVYAWKLILAKEGIVSWFFNIL
                     HLDGVLNSLLSLPVIGGPSLSVSNIGIFIVFVYVWLPYMILPIQAALERVPKSLLEAS
                     GDLGARPMQTFRLVILPLAFPGVVAGSIFSFSLTMGDFIVPTALGNSSYFIGQAVLAH
                     QGTSGNIPLAAAFTMIPIVIMIIYLMVARRLGAFEAL"
     gene            3473..4276
                     /locus_tag="AC16CB_16000"
     CDS             3473..4276
                     /locus_tag="AC16CB_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007817540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3344026.1"
                     /translation="MQKEDRSGDKSPLFLKIAAGAALLFLHMPVLIILLYAFTTDEAT
                     FSFPPPGLTAQWFRIAWGRTDFWDAFLLSLRVAGVATLVALILGTLAAAAVYRSNFFG
                     KESVSFLLVLPLALPGIVTGIALRSALGLMEIPFTFWTIVIGHATFCVVVVYNNALAR
                     FRRLGHSQIEASMDLGANAFQTFRHIVLPNLATALLAGGMLAFALSFDEVIVTTFTAG
                     QQSTLPIWIFSQLTRPRDRPVTNVVATFVIVITFIPILLAHRLTQQDNV"
     gene            complement(4284..5348)
                     /locus_tag="AC16CB_16005"
     CDS             complement(4284..5348)
                     /locus_tag="AC16CB_16005"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3344027.1"
                     /translation="MQITRAEVTPVELKLRQPVALAGHPAIQHITSVFVRIETRQGQS
                     AWGCTIAHPSLTGEKPEQVIHACREYASIASDLHPTNIEYSLSVLTSQVKGDGSALCA
                     FDMAFHDLLGLAAGMPVYRLLGGYRNRIQTSVTIPLATIQESVDIAQARAHLGYRILK
                     IKGGLDPAEDVRRVQAIRRALPDHILRLDADGGYNVQQALDVARALKGKLEMLEQPTP
                     ADDLVSLRQVTEHSPVKILADQSVTGPASALDLAAKRAAGGISIKLAACGGLRCARQI
                     DSIARAARLSTMVSCFIEPALLITAGLSLALSSPNVLYGDLDGNLDLVNDPSKPGFRL
                     EDGWLVAFDTPGLGYSVELN"
BASE COUNT         1112 a         1421 c         1492 g         1352 t
ORIGIN      
        1 ctgagcatcg cacttaattc catcggattg gaggtgatga ttgtttgttg aacatgatgt
       61 gagatacatt gacctgccat agttcgaaaa caaaattatt ccaaaggaga aagtgatgaa
      121 agctaataag atcttgtttc gatggttggt tttgctagtg agtctcagcc ttgtattaac
      181 tgcctgtgga ggtggggcgg tgacaccagc ccccgaggct gagggtggac cgcttcaatc
      241 gattggtgaa ggtgagggcg ctgtctccat catcgcctgg gctggatata tcgagcgcgg
      301 cgagaccgac ccgaacttcg actgggttac ccaatttgag aaggacactg gctgcatggt
      361 gagtgtgaag gtagccgcca cgtccgacga gatggtcgcc ctgatgaacg aaggcggatt
      421 tgacctggtc accgcctctg gcgatgccag taaccgcctg atctcttctg gtagggttca
      481 ggaaatcaac atcgacctta ttccaagctg gagcacggtt gatgaacgcc tgcagaatgc
      541 cccctggaac acggtcgatg ggaagcatta tggcacgcca taccagtggg ggcctaatgt
      601 cctcatgtac aatacagatg tattccccac tccgcctgat agctggagtg tggtcttcga
      661 agaacagacg ttgcccgatg gtcaatccaa caaggggcgc gtccaggctt acgatggtcc
      721 catctacatc gcggacgcgg cgctttattt gatggtcaat aatcctgaat tgggtatcaa
      781 agatccctat aatctggacc gtgaccagtt caatgcttct cttgacctgc tccgccaaca
      841 gcgccagatc gtagggcgct actggcatga cgctttcgtc cagatggatg acttcactaa
      901 cgagggtttt gtcgcctcga gctcttggcc tttccaggtt aacctgctgc aatttgagga
      961 caagccaatc ggcagcgtct tccccaaaga aggcgccacc ggctggtctg ataccaccat
     1021 gatgcatgtg gatgccccgc atcctaactg cgcctacaag tggttagagc attccctcaa
     1081 tcccaagctg caaggcgacc tggctgcctg gtttggttcg gtgccagctg ttcccgcggc
     1141 ttgtacaggc aatgaattgc tcaccgacga gggctgcgac atcaatggca tgaaggactt
     1201 cgataaaatc cacttctgga agacacccac taccgactgt ggtgatgggc gccaggagtg
     1261 cgtgccatat catgaatggg ttactaacta tattgccgtc atcggcgggc gataggtaat
     1321 tcttgaacct cccgcaggat cgatactgcg ggaggttcat ccaggtttgg aataaatgaa
     1381 cgatacagcc gttcccgcag tccgtttatc gaacgtgagt cgcctgtttg gcgaatttgc
     1441 cgcagtggac gatgtcagcc tggacatcca ggatggcgag ttttttacga tgctggggcc
     1501 atcgggctct ggtaaaacga cctgcctgcg tttggtcgct ggttttgacc gtccaaccta
     1561 tgggactatc ttgttgcatg gaaaggatgt cacccaactg ccgccttatg agcgggaggt
     1621 caacacggtt ttccaggatt atgccttatt tccacacatg agtgtgggag ataacgtagg
     1681 ttacgggctg atgatcgata aggtgcccaa agctaaacgg caggagcgag tggaggagat
     1741 gctggaactg gtccagctat ccggtttgac cggtcgcaag ccgtcccagc tctctggtgg
     1801 acaacgacag cgcgtcgccc tggcgcgagc gctgattaaa cacccccgcg tgctgttgct
     1861 agatgagccg cttggcgccc ttgatctgaa attacggcag cagatgcaga tcgaattaaa
     1921 agccatccag cagcgagtgg gcatcacttt tgtctatgtc acccatgatc aagaagaggc
     1981 gctgaccatg agcgaccgca tcgcggtgtt caaccgcggc aaaagcgaac aagttggcac
     2041 cccggcggag atttacgagc atccagcgac tcccttcgtg gctgggttcg tgggtgtctc
     2101 caacctggtg agtggcgaag cagcgcagaa gatcactggt tctgcggata ccttttccat
     2161 cagaccggag aagattcgcc tggcagagcc agatttggat gtgcctgcga acatgtattc
     2221 agccgatggc gctgtgcggg atgtcattta cctgggtctt tacacccgct acctgatcga
     2281 tctggagtta ggtgaggatc tcgtggtgat ccagcagaat ctagacgcaa cttcgatgga
     2341 tgtgctggcg gcgcgcggca agaaagtgcg tctgctctgg ttgcgcgatc ataaccgggc
     2401 tttgaagagc gcgtagaacg atggctactc tctccccatc accgtcaggt gaccttcaaa
     2461 tgagcgtttc tccgggtaag cctgggcgct cttccctgag cacccgagtc tcgtcctacc
     2521 tgtataagcg ccccacggcg atactgcttc tacttctcgg gccgcctatg gcctggatgt
     2581 ttattatcta cctcggttcg ctgttcgccc tgctcgtcaa tagctttttc agcctggatg
     2641 gctataccgg gctggttgtg cgccagttca ccctgaatac ataccgcgac ctttttacgc
     2701 gcgctaattt tgacattgtg caacgcacag ccgggatggc tgctgcggtg accgtcaccg
     2761 cagcgattct ggctttccct ctggcgctgt ttatcgcccg ctatgcatca cggcgggtta
     2821 aagggatcct gtatctggcg gtattgctgc cactctggtc gagctacctg gtgcgggtat
     2881 atgcctggaa gcttatcctg gccaaagagg gtattgtgag ctggttcttc aatattttgc
     2941 atctggatgg cgtgttgaac tcgttgctca gcctaccggt gatcgggggg ccatccctct
     3001 cggtttccaa catcgggata ttcatcgtct ttgtttatgt ctggctgcct tatatgattc
     3061 tccccatcca ggctgcccta gagcgggtgc cgaaatcctt acttgaggca tctggcgacc
     3121 tgggagcgcg gccaatgcaa acctttcgcc ttgtgatcct gcctctggca ttcccgggtg
     3181 ttgtggcggg ctcgattttt tcattctcgc tcaccatggg tgattttatc gtgcccaccg
     3241 cgttaggtaa ttccagctat ttcatcggtc aggcggtgct tgcccaccag ggtacttcgg
     3301 gcaatattcc cctggcggct gcctttacga tgattccgat tgtcatcatg attatttatc
     3361 tcatggtagc caggcgttta ggagctttcg aggcgctcta aacaatgcgt aaatcctgag
     3421 aatggtgtac caatgatgtc aggttacgat agattgatgg agagacgtgc gtatgcaaaa
     3481 ggaagataga tctggcgata aatcaccgct gtttttgaag attgctgctg gggcggcatt
     3541 gttgtttctc cacatgccgg tgttgattat ccttttatac gccttcacca ccgacgaggc
     3601 gaccttcagc ttccctccgc ccggactgac tgctcaatgg ttccgcattg cctgggggcg
     3661 gacggacttc tgggatgctt ttctgctgtc tttacgtgtt gcgggcgtag ctacacttgt
     3721 tgcactgatc ttgggtacac tcgccgcagc ggctgtctat cgcagcaatt tctttggaaa
     3781 agagtccgta tctttcctgt tggttttgcc gttggcgtta ccgggaatcg taaccgggat
     3841 tgccctgcgc tctgctctcg gtttgatgga aatcccgttc accttctgga cgatcgtcat
     3901 tggtcacgcc acgttctgcg tggtggtcgt ttataacaat gcgctggcgc gattccgccg
     3961 ccttggtcat tcccagatcg aggcatccat ggatctggga gccaacgcct tccagacgtt
     4021 ccgtcatatt gttttgccaa acctggcgac tgccttgctg gcgggaggta tgctggcctt
     4081 tgcgctctct ttcgatgagg tgatcgtcac tactttcacg gctggacaac aatccaccct
     4141 gccgatctgg atcttcagcc agcttacgcg gccgcgcgac cgtccggtga cgaatgtggt
     4201 agcgacattt gttatcgtca tcacctttat tcctatcctg ctggcacacc gcttgactca
     4261 acaggacaac gtgtagagat atctcaattt agctcgacag aatagcccaa accgggggtg
     4321 tcgaaggcga ctagccaacc atcttccaat cgaaaacctg gcttagatgg atcattgacc
     4381 aaatccaggt ttccatccag gtcgccataa aggacattag gactgctcaa agccaggctg
     4441 agaccggcag taatcagcaa cgccggctca ataaagcagc ttaccatagt cgacaagcgg
     4501 gcagcccggg cgatggagtc gatctggcgt gcacagcgca atccaccaca ggcagccagt
     4561 ttaatgctta tccctcctgc ggctcgcttt gctgctaggt ccagggctga tgctggacca
     4621 gtcacgctct ggtcggccaa gattttcacc gggctatgct cggtgacctg gcgcagacta
     4681 actagatcgt cggcaggtgt gggctgttcc agcatctcca gcttaccttt cagggcgcgg
     4741 gcaacatcga gagcttgttg gacattgtaa ccaccgtctg catccagacg gaggatatga
     4801 tccggcaggg ctctgcggat ggcttgcaca cggcgcacat cctccgccgg gtcgaggcca
     4861 cctttgatct tcagtatgcg atagcccagg tgggcacgcg cctgggcgat atccacgctt
     4921 tcctggatgg tggctagggg tatggtcact gatgtttgaa tgcgattacg gtagccaccc
     4981 aggagtcgat aaacaggcat gcctgcagcc aatcccaata gatcgtggaa agccatgtcg
     5041 aaggcgcaaa gcgctgagcc atcccctttg acctgactgg ttaacacaga aagggagtat
     5101 tcgatattgg ttgggtgcag atcagatgct atgctggcgt attcacggca agcatggatg
     5161 acctgctcgg gtttctcacc tgtcaagctt gggtgagcga ttgtgcagcc ccaggcactt
     5221 tgcccctggc gtgtttctat acgcacaaat actgacgtga tgtgctgaat cgctggatgc
     5281 ccagcgagcg ctacgggttg tcgaagtttt aattcgacgg gtgtcacttc agctcgggtt
     5341 atctgcattt gcaaacctca taacaaagga tattctt
//
LOCUS       DBNGSX010000544         4143 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1232956, whole genome shotgun sequence.
ACCESSION   DBNGSX010000544 DBNGSX010000000
VERSION     DBNGSX010000544.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1232956"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="AC16CB_16010"
     CDS             <1..348
                     /locus_tag="AC16CB_16010"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3344028.1"
                     /translation="LAMQTPMLVLDEPTTGQDHNDTELVGQIVEDLKEQGRTVITITH
                     DIDFCAEHFDRVVVMAQGMILLDGPARDTLMQTEILAQTHVEPPQLMRLSEQLNMQER
                     PLNVEEFIEFWCP"
     gene            558..1115
                     /gene="efp"
                     /locus_tag="AC16CB_16015"
     CDS             558..1115
                     /gene="efp"
                     /locus_tag="AC16CB_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR3344029.1"
                     /translation="MIDVNALRKGVTFELDDALYKVLDFNHHKPGRGNATIRIKAINL
                     RTGATIDKTFQSGERVQDVRLDYHNVQYLYSDGALYHFMDLETYEQPVISGDVLGDSA
                     EYLKEGMEVKLTFYQGEPLDIELPTSVDLLVTQADIAVRGDTATGVTKKVTTETGLQV
                     SVPSFVEEGNIIRVDTRTGAYVTRV"
     gene            1468..2691
                     /locus_tag="AC16CB_16020"
     CDS             1468..2691
                     /locus_tag="AC16CB_16020"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3344030.1"
                     /translation="MSLTLLLDLDDTLLVNPLKQFLPAYLSKLSTHLAPYAEPSLMVQ
                     ALLAGTRQMVENQQPDCNLRDVFGSVFYPSLKVLPQEIQPAIDQFYTDVFPSLKQLTQ
                     IKPEAVELVDKAFERGYRVAISTNPLFPQTAIVQRLEWAGLSVQKYPFALISSFESFH
                     FSKPNPAFFAEMMARMGWPEDPVLVVGNDLENDIRSATQLGLPTYLVLQPGTPLPVGE
                     GSAPQNTRLRPTASGDLAGLLSWLDETPFETLQPRYDEPSAILAVLRSTPAALNTMCD
                     TLAPAILARRPKPAEWSPSEILCHLRDVDNEVNLPRLKKVLQEDNPFIPGADADRWAE
                     ERAYHQQDGLAALHRFSTSRQELIHLMEKMSPDDWGRPARHAIFGPTNIKELVTINAS
                     HDQIHIRQMVEALRN"
     gene            2931..>4143
                     /gene="fabF"
                     /locus_tag="AC16CB_16025"
     CDS             2931..>4143
                     /gene="fabF"
                     /locus_tag="AC16CB_16025"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003336867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR3344031.1"
                     /translation="MRKRVVITGLGCVSPVGNNVPSMWANILAGISGVDFITHFDASE
                     YKSKIAAEVKGFDASALYGSREARRMDRFTQIALAAAEEARRDAALEIHDGNRDRIGA
                     VIGTAIGGIGTLFEQLQVFNERGPLRVSPFLVPMMIPDTGGGMIAINLGIRGPNMAVV
                     TACATGTNAIGEAAEVIRRGQAEVMFAGGSEAAIVPIAMAGLNVMTALSMQNDEPARA
                     SRPFDLHRDGFVMGEGAAVLVLESLEHAQERGVPILAEFSGYGTTNDAYHISAPAENG
                     AGAALCMRAALNDAGLDTDDINYINAHGTSTPLNDKSETAAIKTIFGEQAYKIPISST
                     KSMTGHLLGASGALEAVICVKALQTQSAPPTINYETPDPDCDLDYVPNSARSMKIDTV
                     MSNSFGFGGHNA"
BASE COUNT         1034 a         1100 c         1028 g          981 t
ORIGIN      
        1 ttagccatgc agacccccat gctggttttg gatgaaccaa ccactggaca agaccacaac
       61 gacaccgagc ttgttggtca aattgtggaa gacttgaaag agcaaggccg gacagtaatc
      121 acgatcactc acgacatcga tttttgtgct gaacattttg atcgagtagt tgtcatggct
      181 caaggcatga tattgctaga tggtccagca cgcgacaccc taatgcaaac ggaaattttg
      241 gcacagacgc atgtcgagcc gcctcaattg atgcgattat ccgaacaatt gaacatgcag
      301 gaacgcccgc tcaacgtgga ggaatttatt gaattttggt gcccttaaca ctcagtatcg
      361 acccctcaat tcctcaaccc ttgccacacc tatttgacta agctataatc tccgcctatc
      421 tggtaaaatc cagatgacga ttgtcattaa taacgcagtt ggaaaagtga tgggggcttg
      481 tgctggctct gcccaaccaa tctctccatc agtcccgacc aactgcgtaa gtctgataaa
      541 ttatgtgagg ctagtttatg attgacgtga acgcattgcg taaaggagtt accttcgaac
      601 tcgacgatgc actctacaag gtgttggatt ttaatcacca caaacctggt cgagggaatg
      661 ccacaattcg catcaaagct attaaccttc gtactggagc caccatcgat aaaaccttcc
      721 agtctggtga acgcgtacag gacgtgcgcc tagattacca caacgtacaa tacttgtata
      781 gcgacggtgc gctttaccac ttcatggatc tggaaaccta tgagcaaccg gttatttctg
      841 gagacgtgct gggagacagc gctgaatacc tgaaagaagg tatggaagtc aagcttactt
      901 tttaccaggg tgagcccctt gacattgaat tacctacctc ggtagacctg ctggtcaccc
      961 aggcagatat cgccgtgcgc ggcgacactg ccacaggtgt caccaaaaag gtgaccaccg
     1021 aaaccggttt gcaagtgagc gtgcccagct ttgtcgaaga aggaaatatt atccgcgtcg
     1081 acacacgtac gggcgcatat gtcactcgcg tttgagcatg cgcacacctg ctggatcaga
     1141 gtgccgctac ttctatgggg attactaccg cggcagaaaa cacgaagagt gccgcctatt
     1201 gaatgattca tctcctcctc tgccatggaa gccagcatat tgttccactt gcccggtacc
     1261 agggattctt cgagcaaacg cgtgcaacga tttatattta acaccccgcc tggggcgccc
     1321 atttccattc ctgaaacagc aggtcgaggt ggatacctat tgcgagaaaa cccagcgagc
     1381 aggttttgat cctcacatcg gctgtggtga atgccatcct attccctcaa tatttactga
     1441 gggagattta cttagtggag acgagaagtg agcctcacct tgctactgga tctggatgac
     1501 actctgcttg tcaatccgtt aaaacagttc ctaccagcat atctgagcaa gttatccacc
     1561 cacctggcgc catacgccga gccatcctta atggtgcagg cgttactggc tggtactcgc
     1621 cagatggtgg agaatcagca accagattgt aacctgaggg atgtatttgg ttcggtattc
     1681 tacccatctc ttaaagtact accccaagag atccaaccag ccattgacca gttttacacg
     1741 gacgtttttc catccctaaa gcagctcacc cagattaagc cagaagccgt cgaactcgta
     1801 gataaggcct ttgagcgagg atatcgcgtt gctatatcca ccaaccctct ctttccccaa
     1861 acagcgattg tccaacgctt ggagtgggct ggtttgtccg tgcagaaata tccattcgcc
     1921 ctgatcagtt cttttgaaag ctttcacttc tccaagccaa atccggcatt ttttgcagag
     1981 atgatggctc gcatgggatg gccagaagat cccgtattgg ttgtgggcaa tgacctggaa
     2041 aatgacatac gaagcgcaac gcagcttgga ttgccaactt accttgtgtt gcaacctggc
     2101 accccgctcc ctgttggaga aggcagtgca ccgcaaaata ctcgccttcg tccaacggct
     2161 tccggggatt tagccggact gctttcctgg ttagatgaaa ctccgtttga aactctccaa
     2221 ccaaggtacg atgaaccatc agccatcctg gctgttctgc gatctactcc tgcggcatta
     2281 aatactatgt gtgatactct tgcccccgca atcttggcaa gacgtcctaa accggcagag
     2341 tggagtccca gcgagatact ttgccacctg cgtgatgtag ataatgaggt aaacctgccg
     2401 cgcctgaaaa aagttctcca ggaagacaat cccttcatcc ctggggcgga tgcagaccga
     2461 tgggcagaag agcgtgctta tcatcagcag gatgggctgg ctgcattaca ccgtttctct
     2521 acctcacgcc aagagctcat ccacctgatg gagaaaatgt ccccagatga ttggggtcgc
     2581 ccagctcgtc acgctatttt tggtccgaca aatatcaaag aactggtcac gataaacgca
     2641 agccacgacc agatccatat ccgccaaatg gtagaagcgt tacgcaattg atcgaagttc
     2701 tgtagaactc gaaactgcgc ctcgattctc tcccattggt gttttaacaa aaagtcggcg
     2761 gaagtgctaa gaaattttcc acagcggtca gcgcagcgcc gtgtttcgtt gctgaccgct
     2821 atatgttgat tgatataata gcggtttatg cagttttgcg ggtcgtgggg ctcacaggct
     2881 cttttcctca aactgcgtaa aagctataat ttcgaccaaa ggaggaagcc atgcgcaagc
     2941 gggtggtaat caccggcttg gggtgcgtca gtccggtggg caacaatgtg cccagcatgt
     3001 gggccaatat cctggctggt atttccggtg tggacttcat aacccatttc gacgccagcg
     3061 agtataaaag caagatcgct gccgaagtga aaggtttcga tgcctcagcg ctgtatggaa
     3121 gtcgcgaggc acggcggatg gatcgcttca cccagattgc cctcgccgcg gccgaagagg
     3181 cacgtagaga tgctgccttg gagatccatg atggcaatcg cgaccgcatc ggtgctgtaa
     3241 ttggcaccgc catcgggggc atagggacgc tctttgagca gctgcaagta tttaatgaac
     3301 gtggaccgct gcgagtcagt ccattccttg tgcccatgat gattcctgac actggtggtg
     3361 gaatgattgc gatcaatctg ggcatccgtg ggcctaatat ggcagtggtc acggcctgcg
     3421 ccacaggcac aaacgccatt ggggaagcag ctgaagtgat ccggcgcggc caagctgaag
     3481 taatgtttgc aggtggaagt gaggcggcta tcgtacccat cgctatggct ggcttgaacg
     3541 tcatgaccgc cctatccatg caaaatgatg aacccgcacg cgcttcccgc cccttcgacc
     3601 ttcaccgcga cgggttcgtt atgggtgaag gagcagctgt cttggtgctc gaatccttgg
     3661 aacatgccca agaacgtggg gtgcccatcc tggctgagtt ttctggctat ggcaccacga
     3721 atgacgccta tcacatctct gcgcctgcag agaacggcgc cggtgcggcc ttatgcatgc
     3781 gcgcagcctt aaacgatgcc gggttagata ccgatgatat caactacata aacgctcacg
     3841 gcaccagcac ccccctaaac gataagagcg agacagcagc catcaagacc atctttggag
     3901 agcaagctta taagatccca atatcctcta ccaaatcgat gacaggccac ttgctggggg
     3961 cttccggcgc actggaagct gtgatctgtg tgaaagccct ccagacccaa tctgcccctc
     4021 ccaccatcaa ctatgagaca ccagatccag attgcgatct ggattatgtc cctaattctg
     4081 cccggtcgat gaaaatcgac acggtgatgt ctaattcatt tggatttggc ggccacaatg
     4141 cct
//
LOCUS       DBNGSX010000545        11143 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_887441, whole genome shotgun sequence.
ACCESSION   DBNGSX010000545 DBNGSX010000000
VERSION     DBNGSX010000545.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11143)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11143)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11143
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_887441"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="AC16CB_16030"
     CDS             <1..303
                     /locus_tag="AC16CB_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3344032.1"
                     /translation="ILSHADHTGVPRNTRMEKALLACDEITGLITAVALVRPSRSLHD
                     LEAGSVKKKWKDRAFAAGANRGEISHAVEDFGIDLWEHVGNVIQAMRRIAPQLALD"
     gene            418..2364
                     /locus_tag="AC16CB_16035"
     CDS             418..2364
                     /locus_tag="AC16CB_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR3344033.1"
                     /translation="MIKVEEENRGSVVLRRAAEYTPPSSWRTWLIGRPLQTADAPHQT
                     IGKAIGLAVFASDALSSTAYATQEILIILAVAGTVAFGYVFPISIAIVILMAIVTISY
                     QQTIHAYPGGGGAYIVSRDNLGELPAQIAGAALLTDYILTVAVSVSSGVAQIVSAYPE
                     IFAYRVHISVILVFFIMLINMRGVKESGAAFALPTYFFVVMMYITVGVGLFRALTGSL
                     GPVIDPPELEAIHALQGVTAFLILHAFSSGTAALTGIEAISNGITAFKEPRSRNAGIT
                     LIWMALILGSLFLGISYLAREIGTIPSEIETVISQLARTIFHGQAGLYLAVIAATTVI
                     LVMAANTAFADFPRLSALLAADGFLPRQLTYRGSRLVFSRGIALLALVASLLIIVFNA
                     SVTRLIPLYAIGVFSSFTLSQAGMARRWWKIGHLEPGQELKERGSMLRYESGWQLKMV
                     INGFGALCTLVVMFVFAITKFRDGAWIVLILTPSLVAIFSSIHQHYRNLAKRLSLDNY
                     GLPPRIPRHRVILPISGVHRGSLAALQYARTLSDDITAVHVCTDPEEASKTQQKWEKW
                     GDGVRLVLLDSPYRLLAEPLLEYIESIANQRQPNEIITVIVPQFVPKRWINNLLHTQT
                     ALWLRLALLFKPGIVITDVPYQVE"
     gene            2380..3039
                     /locus_tag="AC16CB_16040"
     CDS             2380..3039
                     /locus_tag="AC16CB_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR3344034.1"
                     /translation="MNIIIVGCGRVGAELAYRLYQKGHNISVIDHVASAFNNLPSDFR
                     GRTIEGEVMNMDVLSRAGIDSADGLAAVTNSDPLNAVVAHLARSAYGVPNIVVRNYDP
                     SLRVLHETFDLQVVSSSSWGAQRLEEMLASVNLRTVFSAGNGEVEIYEFSVPAAWDGA
                     TLGELLPTTNGIIISVTRAGKAILPTPDYQIKTNDVILVSATFDGIEVLRNILHSRPE
                     A"
     gene            3042..3737
                     /locus_tag="AC16CB_16045"
     CDS             3042..3737
                     /locus_tag="AC16CB_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR3344035.1"
                     /translation="MFVIIIGGGRTGTQLATLLLNQNHHVHIIENRREVLSRMHRELP
                     TEIIYEGLFSSPAVLEQAEIQKADVLAACTVNDAENLVACFLGRTRYGIGRTIARVNN
                     PRNAWLFDEKFCVDVALNNAEIMARLIEEEMSMGDMMTLLKLRRGDFSLVEEKIPPGA
                     PAIGIAIQNLGLPDNCIIAAIVRRGQMLVPRGITTFEAGDEVLAITDSQGAEQLASLF
                     TPSESKSKGSNPI"
     gene            3834..4010
                     /gene="rpmH"
                     /locus_tag="AC16CB_16050"
     CDS             3834..4010
                     /gene="rpmH"
                     /locus_tag="AC16CB_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIR3344036.1"
                     /translation="MPKRTYQPKIRRRVRVHGFRSRMATAGGRKVIKNRRLKGRSRLG
                     VTKNNHVKKVNWNA"
     gene            4060..4407
                     /gene="rnpA"
                     /locus_tag="AC16CB_16055"
     CDS             4060..4407
                     /gene="rnpA"
                     /locus_tag="AC16CB_16055"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR3344037.1"
                     /translation="MKRDFRLRKSADFERVRRSGKSFAHPLVVLIVHDNPGEGVRIGV
                     AAGRSLGMAVQRNRAKRMIRAAVHSMLNHIRPGHDLILIARRPMLEADFHKLQEALLT
                     LLRRAQILNANDD"
     gene            4484..4690
                     /gene="yidD"
                     /locus_tag="AC16CB_16060"
     CDS             4484..4690
                     /gene="yidD"
                     /locus_tag="AC16CB_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013299151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIR3344038.1"
                     /translation="MILLGLIRLYQMTISRGLPPNTCRFHPSCSHYGYQAIYKYGALK
                     GSLMAAWRVLRCNPFNPGGYDPVK"
     gene            4739..5839
                     /locus_tag="AC16CB_16065"
     CDS             4739..5839
                     /locus_tag="AC16CB_16065"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3344039.1"
                     /translation="MKSKHLLILIILLISSVVLSACAGGAATAASSWPDLTVDGETAF
                     LSYNQHVYAIDLANGTQKWRFPLEADNKISFYAAPVITPDGQLLVGGYDHVLYSLNPQ
                     TGTENWSFKNAKNRYIGSPLASEAGIFAPTADDNLYALDNQGNARWSFATQGAQWAKP
                     VSDPNCECIYLPSMDHHLYAINAQTGAQEWKSEDLGGSVVGTPAYAQDDTLYIGTFNS
                     EMLAIDANNGRIRWRVTTDGWIWGGPVLKDGALYFGDLSGAFYAMDAADGKVRWKIKP
                     DGPISQSPLLTEESIYFTTEAGSLYGVSYNGDIRVNQPIACQPNGACPKLHTSPVSAG
                     DKILVAPTGVEQLLIALDANGEQIWSFIPEKK"
     gene            5854..6612
                     /locus_tag="AC16CB_16070"
     CDS             5854..6612
                     /locus_tag="AC16CB_16070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HIR3344040.1"
                     /translation="MWDMLIINPMVNTLLWIYSVLWQNFGLAIIVFTLVVRLITYPLT
                     AQQMKSAQAMQDMQKSKKWQDIQKKYKDNREKLAQEQMSLYKEMGVSPFGSCLPTLIQ
                     FPIIIGLYQGIIRALAVTPIQLLDLSTHIYPFINASDLIPLHNRFLWMDLSQPERLYV
                     FGFGVPVLAILVVITTYFQSKLMTPMTQPGDQGAQMTQAMNLYMPFLMGWLAYSFSSG
                     LALYFVASNVFTILQYAAMGKLQWSNLLPTRKSR"
     gene            6672..7490
                     /gene="jag"
                     /locus_tag="AC16CB_16075"
     CDS             6672..7490
                     /gene="jag"
                     /locus_tag="AC16CB_16075"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HIR3344041.1"
                     /translation="MRKSRTTLEVIAPTIEEAVIKGLDELGLPQDAVDVEILDEGSKG
                     LFGLGSRQARVRLTVKTTSPGEEAGEEKPARAKRAAKRTPQKVSEVQEDVLPSEMEAG
                     EEDESTTAIKAEPQDDYILQIAEAVVAELLHRMRVRADVSAEFGEADDRSHRVPVLVN
                     ITGQDLSILIGRQTETLNALQYIAGLIVSKETGRPLTLIIDVQGYRKRREQQLRQLAR
                     RMADQAVKTGRRQVLEPMPANERRIIHIELRDYPEVSTESIGEEPRRKVTILLK"
     gene            complement(7543..7992)
                     /gene="aroQ"
                     /locus_tag="AC16CB_16080"
     CDS             complement(7543..7992)
                     /gene="aroQ"
                     /locus_tag="AC16CB_16080"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015170084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIR3344042.1"
                     /translation="MKPILVLHGPNLNLLGLREPEVYGSLTLEEIDRRLVEVGREIGL
                     EVRALQFNGEGDLIDALHQARDWARGVIFNPGGYTHTSVALRDAVASIGLPVVEVHLS
                     NVHAREEFRRQSMIAPVCVGSIVGFGWRSYWLGLHSLAAILNKSDIK"
     gene            complement(8064..9617)
                     /gene="aroB"
                     /locus_tag="AC16CB_16085"
     CDS             complement(8064..9617)
                     /gene="aroB"
                     /locus_tag="AC16CB_16085"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR3344043.1"
                     /translation="MSSGRDFICLYGPPGSGKSSVGRALAARLGFPFWDLDEEIQVRS
                     KMNIPEIFAREGEKGFRRRERKVLLDLLAHQEGVLALGGGALLNPANLTQVEAAGTVL
                     CLQAPIERLLERLGAESNSRPLLNGDLRPRLESLLSSRADHYNSFPFALDADWPVERI
                     VRDAQIRIGKFRVSGMGQAYDVIVQESGLDGIGDLLLKRGLRSPVIVVSDENVGSLYT
                     QQVLDSLQSANYRAHSITIPPGEQHKNLHTVASLWEGFLAAGIERSSTVIALGGGVVG
                     DLAGFAASTILRGVPWVALPTTLLAMADASLGGKTGFDLPQGKNLVGAFHSPRLVLAD
                     PHTLQTLPKAELRAGMAEVVKSGLVGDAELFALCGRGWEAVERDLGEVVRRAMAVKIS
                     VIEDDPYEKGRRAALNLGHTFGHAVELASGFQLRHGEAIAIGLVAEARLAEKIGIAET
                     GLAGEIEGVLRALGLPVEIPPELDRVAVREVMQVDKKRLDGRVRFALPIRPGEVKVGV
                     VVDFTELSI"
     gene            complement(9601..10782)
                     /gene="aroC"
                     /locus_tag="AC16CB_16090"
     CDS             complement(9601..10782)
                     /gene="aroC"
                     /locus_tag="AC16CB_16090"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR3344044.1"
                     /translation="MSLHFMTAGESHGPALVAILEGMPAGLAISMEGIQADLARRQRG
                     YGSGPRMKIEKDTARVLSGVMDFGDGPLTTGAPLSILIENIDHEKWRGRSIEPLTTPR
                     PGHSDLSGALKYGYRDLRPALERSSARETAARVALGAVCRQFLAQFDIQVGGYVASIG
                     EIIADVGSIPIAERIHRAEQNDVSCPEVSAAEVMHARIRRAMEDRDTLGGIIEVFALG
                     VPPGLGSYVHWERRLEARLGAAVLSVQAIKGVEIGPGFENTRLPGTQAHDAIQLQGEN
                     LVRPTNRSGGLEGGITTGQPLHVRAAMKPIATTLTPQQTVDLEKGVETPTQYERSDFC
                     PVPRAVVIIESMVAFVLADALIEKLGGDSLEEMRPRFEALRRARLSDLPMDGSEHVFW
                     P"
     gene            complement(10779..>11143)
                     /locus_tag="AC16CB_16095"
     CDS             complement(10779..>11143)
                     /locus_tag="AC16CB_16095"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3344045.1"
                     /translation="DSGHFLPVLDAAIEAMCGDQSWLGDRNKIYRQRRDAALQGLRGM
                     GLKAETPRASIYVWSAVPEGWSSEDFASAALEGAHVSVTPGTVFGDAGEGYMRISLTA
                     PVERISQAMSRLERWLKR"
BASE COUNT         2668 a         3197 c         2778 g         2500 t
ORIGIN      
        1 atcctcagcc acgccgatca cacaggtgtg ccacgcaata cccgtatgga gaaagccctg
       61 ctggcctgcg acgagatcac cgggttgatc acagcggtgg cacttgtgcg cccatctcgc
      121 tcgttacatg acctcgaggc gggatccgtg aaaaagaagt ggaaagaccg cgcctttgca
      181 gccggtgcca accggggaga aatctcacac gcggtagaag atttcggcat cgatttgtgg
      241 gagcacgtgg ggaatgtaat tcaagctatg cgccgcatcg ccccacagct agccttggat
      301 tgaaatttcc ttattgacgc ttcgttcgat attgaataga atcaaagagc ttaccagccc
      361 gccgggtgct atcttcggat cgcggtgggc tttcgtttgc tattaaggaa aatgcacatg
      421 attaaggttg aagaagaaaa tcgcggctcg gttgttttaa ggcgcgccgc agaatacaca
      481 ccgccttcgt cctggcgcac gtggttaatt ggtcgcccgc tgcaaaccgc tgacgcgccg
      541 catcaaacga ttggcaaagc catcgggctg gcagtattcg cctccgatgc tctctcgtct
      601 accgcatacg ccacccagga aatcttgatt atcctggctg tagcgggtac cgttgccttt
      661 gggtacgtat tccccatctc catcgcgatt gtgatcttga tggcgatcgt aaccatatcg
      721 tatcagcaga caatccacgc ctatccaggg ggaggtggcg cctatattgt ctcgcgagac
      781 aatctgggtg aattacctgc ccagatagcc ggtgccgcgc tgctcaccga ttacatcttg
      841 acagttgcag tttctgtctc atctggggta gcgcagattg tctccgctta cccagagata
      901 ttcgcttacc gagtgcacat ttccgtgatc ctagtatttt ttattatgtt gatcaacatg
      961 cgcggcgtta aagaatcagg cgcggcgttt gcactcccta cctacttttt tgttgtcatg
     1021 atgtacataa ctgtcggagt tgggctattt cgagccttga ctggctcctt gggacctgta
     1081 atcgatccac ccgaattaga agctattcac gccctgcaag gggttacggc gttcctgata
     1141 ctacacgcat tttccagcgg cacagctgct ttgactggaa tagaagccat atcgaatggc
     1201 atcacagcat ttaaagaacc gcgcagccgc aacgctggca tcacattgat ctggatggct
     1261 ctcatcctgg gttccctgtt cctggggatt tcgtacctgg cgcgcgaaat tggcaccatc
     1321 ccatccgaga tcgaaaccgt catctcgcag ctcgcccgca caatatttca cgggcaagca
     1381 ggtttgtacc tggctgtaat tgcggccacc accgtaatat tggtcatggc agccaatacc
     1441 gccttcgcag atttcccccg cctcagcgcc ttgctcgctg cggatggctt tttaccacgc
     1501 cagttaacct accgtggcag cagactggtg ttctcacgag gcattgcgct tctagccttg
     1561 gtagcctcgc ttttgattat tgtattcaac gccagcgtga cccgtttgat ccctttgtac
     1621 gctatcggcg tgttcagttc attcacgcta tcgcaagctg gcatggcgcg caggtggtgg
     1681 aaaattggac acctggaacc aggtcaggaa ctcaaagaac gcggttcaat gcttcgttat
     1741 gaatccggct ggcaactcaa gatggttatc aatggattcg gagcgttgtg caccctggtg
     1801 gtcatgtttg tatttgcaat aaccaagttc cgcgatgggg catggatcgt cctgatccta
     1861 accccaagcc tggtcgccat tttctcctcc attcaccaac attacaggaa tttggcgaaa
     1921 cgcctctcgt tggataatta tggtctgccc ccacgtatcc caaggcaccg cgtcatcctg
     1981 cccatcagtg gtgtgcaccg gggttcattg gcagccttgc aatacgcgcg tactctgagc
     2041 gatgatatca ccgccgtgca cgtttgcacc gacccggaag aagccagcaa aacccagcag
     2101 aagtgggaga aatgggggga tggcgtgcgc ctggtgttgc tcgattcacc atatcgcttg
     2161 ttagctgaac ccttgctgga gtacattgaa tcgattgcca atcaacgcca accaaacgag
     2221 atcatcactg tcatcgtgcc acaatttgtc cccaaacgct ggataaacaa tctgctgcac
     2281 acccaaaccg cactctggtt gcgattggcg ttattattta aaccagggat cgtcatcacc
     2341 gacgtgcctt atcaagttga gtaaaggagc ctctctcaaa tgaatatcat catcgtcggt
     2401 tgtggtcggg taggtgcaga attagcctac cggctctacc agaaagggca taacatctcc
     2461 gtgatcgatc atgtggcatc tgcgttcaat aaccttccct cagattttcg tgggcggacg
     2521 atagaaggcg aagtgatgaa tatggatgtg ctcagccgtg cggggattga tagtgccgat
     2581 ggtctggcgg cggttaccaa ttccgatcca ttgaacgccg tcgtggctca tctggcgcga
     2641 agtgcatacg gcgtcccgaa catcgttgtg cgaaattacg acccaagcct gcgtgtcctt
     2701 cacgagacct ttgacttgca agtagtcagt tcctccagtt ggggagccca acgcctggaa
     2761 gagatgcttg cttccgtaaa tttgcgtacc gttttttctg caggtaatgg tgaggtagag
     2821 atctacgagt tcagcgtacc agctgcttgg gatggcgcaa ccttgggtga attgctccct
     2881 acaacgaacg gcatcatcat ctccgtgaca cgtgccggga aagccatctt gccaacccca
     2941 gattaccaga tcaaaacaaa tgatgtcatc ctggtaagtg ctacgttcga tggcattgaa
     3001 gtattacgta atattttaca cagtagaccg gaggcgtaac catgtttgtg atcatcattg
     3061 gcggcgggcg caccggcacg caactggcaa cactgttgct taatcagaac caccatgtcc
     3121 acatcatcga aaatcgccgt gaagttctta gccgcatgca ccgcgagttg cccaccgaga
     3181 tcatatacga agggctcttt tccagcccag ctgtgcttga acaggctgag atccagaagg
     3241 cagatgtgct cgctgcatgt acagtcaacg atgcagagaa cctggtagcc tgcttcctgg
     3301 ggcgcacccg atacgggatc gggcgcacaa tcgcgcgagt caataaccca cgcaacgcct
     3361 ggcttttcga tgaaaaattc tgcgtggacg ttgctctaaa taacgccgag atcatggcaa
     3421 ggttaatcga ggaagagatg tcgatgggcg acatgatgac cttgctgaag ctgcgccgcg
     3481 gtgacttctc attggttgag gaaaaaattc cgccaggggc accagccatc ggcatcgcca
     3541 tccaaaattt gggtcttccg gataactgca ttatcgcggc catcgtccga cggggtcaga
     3601 tgctcgtgcc acgtggcatc acgaccttcg aagctggcga tgaggtattg gcgatcaccg
     3661 acagtcaggg agcggagcaa ttggcaagct tgttcacccc ctccgaatca aaatcgaagg
     3721 gatcgaatcc catttgacca aacttcttcc atagggtata atcgcctttc gtctgttatc
     3781 gtacagttta taataaatgc tgctatcatt aacttaggaa cggaattaca actatgccga
     3841 aacgaactta ccagcccaaa atacgccgca gagtgcgcgt ccacggtttt cgtagccgca
     3901 tggcaactgc cgggggccgt aaagtgatta agaaccgccg cttgaaaggc cgcagccgct
     3961 taggcgttac caagaacaat cacgtcaaga aggttaattg gaatgcataa tcaggcagga
     4021 gcggatgaaa catcagcatg atctggctcc gtcagacagg tgaaacgcga ttttcgactg
     4081 agaaaatcag ccgatttcga gcgggtgagg cggtcgggaa agtcttttgc ccacccgctt
     4141 gtcgtgttga ttgtgcatga caacccagga gagggtgttc gcatcggagt tgccgccgga
     4201 cgtagcttgg gaatggctgt ccagcgcaat cgagcgaaac gcatgatacg cgctgccgtt
     4261 cattctatgc taaatcacat ccgccctggt cacgatctca tcttgattgc tcgtcgtccc
     4321 atgcttgagg ctgacttcca taagctgcaa gaggcactgc tcacactgtt acgccgcgcc
     4381 cagatactga atgcaaatga cgactgaagt agaaacaaca cacgaatata catctgagcc
     4441 acgcttgcgc gatctgcctc tcagcctgca aaatatcccc cgcatgatct tgctgggatt
     4501 aatccggcta tatcaaatga ccatctcgcg tggcctgcca ccaaacacat gccgcttcca
     4561 tccatcttgc tctcattacg gttatcaggc aatatacaag tacggtgcat taaaaggcag
     4621 cctcatggct gcctggaggg ttttgcgctg taatccattc aacccgggtg gatacgatcc
     4681 tgtaaagtaa ttaatggatc aaaccatgcc tgaggatttt cgaatttata agcgaaatat
     4741 gaaatctaaa catctactaa tactcatcat cttgctcatt tcctccgtcg tactaagcgc
     4801 ctgtgctggg ggcgctgcca cagccgcttc cagctggcca gacttgacag tcgacggaga
     4861 gaccgctttt ctgtcataca accagcatgt ttatgccatt gacttagcta acggcacgca
     4921 gaagtggcgc ttccctctcg aagccgataa caaaataagc ttctacgccg ccccggtcat
     4981 tacccctgat ggacagttat tggtaggggg ttacgatcat gtcctataca gtctaaatcc
     5041 ccagacaggg acagaaaact ggtcattcaa aaacgctaaa aaccgctata ttggcagccc
     5101 gctggcaagc gaagcaggaa tttttgcgcc aaccgctgat gacaacctct acgctctcga
     5161 caaccaggga aatgctcgct ggtcctttgc gacgcagggc gcgcagtggg caaagccggt
     5221 ttcagatccc aattgtgagt gtatctacct cccttctatg gaccatcatt tatacgccat
     5281 aaacgctcaa acgggcgcgc aggaatggaa atccgaagac ttaggtggat cggtcgtagg
     5341 gaccccagct tatgcgcagg atgatacact ttatatcggg accttcaaca gcgaaatgtt
     5401 ggcgattgac gctaataatg gtcgcatccg ctggcgagta actacggatg gatggatttg
     5461 gggtggccca gtcttaaagg atggtgcgct gtatttcggc gatctgagcg gtgcatttta
     5521 cgccatggat gccgcagatg gcaaggttcg ctggaaaatc aaaccggatg gtccaatttc
     5581 ccaatctcct ctgctcaccg aagagtcgat atatttcaca actgaggcgg gatctctgta
     5641 tggtgtaagc tataatggcg atattcgcgt caaccaacct atcgcctgcc aacccaacgg
     5701 ggcatgcccc aagttgcaca catcccctgt cagcgcaggt gataaaatct tagtggcacc
     5761 caccggggta gaacaattac tcatcgccct ggatgcaaat ggcgagcaaa tctggtcatt
     5821 cattcctgag aagaaataac ggaagatatt gatatgtggg atatgctgat tatcaaccca
     5881 atggtcaata cgctgctatg gatttacagc gtgctttggc aaaattttgg cctggcgatt
     5941 atcgtgttca ctttggttgt gcgcctgatc acctatccgc tcacggccca gcagatgaaa
     6001 agcgctcagg cgatgcagga catgcaaaaa tcgaagaagt ggcaggatat ccaaaagaaa
     6061 tataaggata accgcgaaaa attggcacag gagcaaatga gcctatataa ggagatgggg
     6121 gttagcccat tcggctcctg cctgccgacg ctgatccagt tccctatcat tatcggcctc
     6181 taccagggca taatacgcgc tctggcggtt acccccatcc aattgctcga tctctccacc
     6241 cacatttacc catttatcaa tgcctctgac ttgattccac tccataaccg ttttctgtgg
     6301 atggatctca gccagcctga gcgactgtat gttttcggat ttggcgtgcc ggtgctggca
     6361 atattagtgg tcatcaccac atatttccag tcaaagctga tgaccccaat gacgcagccc
     6421 ggcgaccagg gagcccaaat gacccaggcg atgaatttat acatgccatt cctgatgggt
     6481 tggctggcct attcattctc atcagggtta gccctgtact ttgtggctag caatgtcttc
     6541 accattctcc agtatgcagc catgggcaaa cttcaatgga gcaacctgct accaacccgc
     6601 aaatcccgat gaggataact taagaattag tgcccgtcct accaagcagg acaattattg
     6661 aagaggccat catgcggaaa tcacgtacaa ctttagaggt aattgcgcca actatagagg
     6721 aagccgtcat caaggggctg gatgaattag gattacccca agatgcagtg gatgttgaaa
     6781 tcctggacga aggtagcaaa ggactatttg gcctgggttc ccggcaggca cgcgtccgtt
     6841 taactgtcaa aaccacctcc ccaggtgaag aagcaggtga ggagaagccg gcccgggcta
     6901 aacgtgcggc caaacgcacc ccccagaagg tctccgaagt gcaggaagac gtcttgccaa
     6961 gcgagatgga agcgggagag gaagacgaat caactactgc gatcaaggca gaaccgcaag
     7021 acgattacat actccagatt gctgaggctg ttgtcgcaga gctgcttcat agaatgcgcg
     7081 tccgtgccga tgtcagcgct gaattcggtg aagccgacga ccgcagtcac cgggtgccag
     7141 tgctggtgaa tatcaccggt caagacttga gcatcctcat cggacgccag acagaaaccc
     7201 taaacgcctt gcagtacatt gccgggctga tcgtcagcaa ggagacaggt cgccccttga
     7261 cgctgatcat tgatgtgcag ggctatcgaa agcggcgcga gcagcagcta cgccagctcg
     7321 cacgccgtat ggctgaccag gctgttaaga ccgggcgccg ccaggtcttg gagccaatgc
     7381 ccgccaatga gcgccgcatt atccacatcg agctacgcga ctatcctgag gtttcaacag
     7441 aaagtattgg ggaagagccg cgacggaaag tcaccatcct gctcaagtaa actcgctcag
     7501 ttcttcaccc cttcgggatt cgatcttcga attactctag tcctacttaa tatccgattt
     7561 gtttagtatc gccgccaggg aatgcaatcc caaccagtaa gagcgccaac caaatcccac
     7621 gattgatcct acacaaaccg gcgcgatcat cgattggcgg cgaaattcct ctcttgcatg
     7681 cacattggag agatgcacct ctacaaccgg taacccgatc gaggcgacag catcccttaa
     7741 cgccaccgat gtgtgcgtat atccccccgg gttaaaaatc accccacgcg cccagtcgcg
     7801 cgcctggtgc aaggcatcaa tcaaatcccc ctcgccatta aattgtagcg cacgcacctc
     7861 caaccctatc tctcgcccca cctcgaccaa gcgtctatcg atctcttcca gtgtcagtga
     7921 gccataaacc tcgggctccc ggagcccaag tagatttagg tttgggccgt gtaaaacaag
     7981 tatcggtttc attttccact cattggtgct gcatcataag gttcttcaga tcctgactgg
     8041 tagtattgct tcccctatat atcttatatc gatagctccg taaaatccac aaccacaccc
     8101 accttaacct cgcctgggcg gataggcaaa gcgaagcgca ctcttccgtc gagacgtttt
     8161 ttatccactt gcatcacctc ccgaactgca acacggtcca actccggggg aatctctacc
     8221 ggcaagccta gcgccctcaa gacaccttcg atctcgccag ccaaccccgt ctcagcaatg
     8281 ccaatcttct cagccaggcg cgcctcagca accagaccaa tggcgattgc ctccccgtgt
     8341 ctcaattgaa agcccgacgc cagctcaaca gcatggccaa aggtatgccc caagtttaac
     8401 gctgcccggc gacccttttc atagggatcg tcttcaatga ccgagatttt caccgccatc
     8461 gccctgcgca ctacttcgcc caaatctcgc tctacagcct cccagccgcg accacatagg
     8521 gcgaacagct ccgcatcgcc aaccaagcca gatttgacca cttcggccat acccgctctc
     8581 aattcagcct ttggcagggt ctgcaaggta tgcgggtcag ccagcacgag gcgcggcgag
     8641 tggaaggctc caaccaagtt tttaccctgg ggcaggtcaa atccggtctt gccgcccagg
     8701 ctggcatcag ccattgccag tagcgttgtg ggcagcgcca cccagggcac gccgcgcaag
     8761 atcgtcgaag cggcaaatcc agccaaatca ccaaccactc cgcctcccaa ggcaatcacc
     8821 gtgctgctgc gctcgatccc cgcggccaga aaaccctccc acaaggaagc cacggtgtgt
     8881 agattcttgt gctgctctcc tggtggaatg gtaatagaat gcgccctgta gtttgcagat
     8941 tgtaacgaat ccaaaacttg ttgggtatag agcgaaccta cgttctcatc gctcactaca
     9001 attaccgggc ttcttaatcc ccgtttgaga agcaaatctc caatgccatc taaaccactt
     9061 tcctggacaa tcacatcata tgcctgcccc atgccactca cacggaactt gccaatgcgg
     9121 atttgagcat cgcggacaat ccgctcaacc ggccaatcgg catccagggc gaatggaaat
     9181 gagttgtaat gatcggctcg gctggataac aagctttcca gacgaggccg caggtctcca
     9241 tttagcagcg gacgagagtt gctctccgcg ccaagacgtt ctaataatcg ctcgatggga
     9301 gcttgcaggc ataacacagt tcccgcagct tcgacctggg tgagattggc tgggttaagc
     9361 agagcgccac cgcccaaagc cagcacacct tcctggtggg ccagcagatc aagcaagact
     9421 ttccgctcgc ggcggcgaaa acccttctcg ccttcccggg cgaaaatctc cgggatattc
     9481 atcttgctcc tcacctggat ttcttcatcc aaatcccaga acggaaagcc caatctagcc
     9541 gcgagggctc gtcctacgct gctcttgccc gagcccggag ggccatatag acagataaaa
     9601 tcacggccag aagacatgct cgctcccatc catgggcagg tcagagaggc gggcgcggcg
     9661 cagagcctcg aagcgggggc gcatctcttc taacgaatcg cctcccagct tctcgatcag
     9721 cgcatccgcc aacacaaagg caaccataga ttcgataata acaacggcgc gcggcaccgg
     9781 gcagaaatcg gaacgctcgt attgagtggg agtctccacc cctttctcca agtctaccgt
     9841 ttgctggggc gtcagcgtgg tagcgatggg tttcatggca gctcgcacgt gcagtggttg
     9901 cccggttgta atccccccct ccagccctcc agatcggttt gtcggtcgca ccagattctc
     9961 cccttgaagt tggattgcat catgagcctg cgtgcccggc agacgggtat tttcaaaccc
    10021 cggaccaatt tcaaccccct tgatagcctg cacactgaga actgctgccc ccaggcgggc
    10081 ttccaggcgg cgctcccagt gcacataaga gcctaaacca ggtgggactc ccaaggcaaa
    10141 aacctcgatg atccctccca gtgtatcccg atcttccata gcacggcgta ttcgggcgtg
    10201 catgacctca gccgcagata cttccgggca ggagacatca ttctgctccg ccctgtggat
    10261 gcgttcggca atagggattg agcctacatc ggcaataatc tcgccgatcg aggccacata
    10321 cccccccacc tgaatatcga actgcgctaa aaattggcgg cagacagctc ccagcgcgac
    10381 ccgcgcagca gtttcacgag ccgaagaccg ctcaagcgct ggtcgcaaat ctcggtatcc
    10441 atatttgagt gccccgctca ggtcggagtg tcccgggcga ggggtcgtca aaggctcaat
    10501 gctacgccca cgccatttct catggtcaat attctcaatg aggatggaca gcggcgcacc
    10561 ggttgtcagc gggccgtcgc cgaaatccat aacaccactc aatacccgcg ccgtatcctt
    10621 ctcgatcttc atacgtggac cagatccgta accacgctgg cggcgagcca ggtctgcctg
    10681 gataccttcc atcgatatgg ctaacccagc aggcatcccc tccaggattg cgaccagtgc
    10741 cggcccgtga gattcacccg ccgtcatgaa atgcaaactc atcgtttcaa ccacctttcc
    10801 agacgagaca tagcctggct gattcgctcg accggggccg tcaatgagat acgcatatac
    10861 ccctctcccg catccccgaa cacggtgccg ggcgtcacac tgacgtgtgc accttccagc
    10921 gcagcactgg caaaatcctc ggatgaccag ccctccggca cagcactcca cacatagatt
    10981 gaggcacgcg gtgtctcagc ctttaatccc atgccacgca agccctggag agccgcatcc
    11041 cgccgctggc ggtagatttt attgcgatcg cccagccagc tctgatcgcc acacatcgct
    11101 tcgatggcgg catccagcac aggcaaaaaa tgaccgctgt cca
//
LOCUS       DBNGSX010000546         3903 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_124785, whole genome shotgun sequence.
ACCESSION   DBNGSX010000546 DBNGSX010000000
VERSION     DBNGSX010000546.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_124785"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..3081
                     /locus_tag="AC16CB_16100"
     CDS             124..3081
                     /locus_tag="AC16CB_16100"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3344046.1"
                     /translation="MTKLALYLLGAPLLENDGQLVAFDTRKAMALLAYLAVTTKVHSR
                     DSLAALFWADYAQSSARAALRRTLSVLNKALREDTLEITRDRLGLAQGAALWFDVEEF
                     QRLLKERETHGHPQREICSRCLDCLEKAVDLYRGDFMAGFTLRDSPEFDDWQYFQTDS
                     LRRELAGALEQLAHGLAAKAEYERAIVHARHRVALDPLHEAAHRQLMQLYAWAGQQSA
                     ALRQYQECTRILDKELGVPPMPETTQLYQAIKEQRAQEPLTTEYQFAERPQSLESIAP
                     PPSRAAAHMLPLVGRWQEMASIKDNYAGIQSDGYFQILEGEAGIGKTRLAEEFLSEAG
                     TRGAFSIMTRCYSGESNLAYAPFLEGLRAALAHAHPNWHQPIQAHWLVEAARLLPELS
                     RLRPDLPAAPSLDSPGAQTRFFEGVSQVLQALCDSQPPGILFFDDLQWADEASLDLLT
                     YLVRRLHGRRLFVLAVWRGEDLPMEHRLRRLLAEAQRNGYASAITLSRLDTKSIMELV
                     KFVAGEAGLPLENMTLRLFEETEGLPFFITEYLATLPEVDTGLQDDWTMPQGVRDILH
                     SRIDALDDAGRQLLQTAAVIGRSFDFETLQYASGRSEEETTTTLEALIASGLIRETQP
                     GRERRASDLLRTLDYDFSHDKTRALVYEETSQGRRRLLHQRVADGLLRHSRSRQGMRL
                     VAGQLAYHYLQSGRAKEAAEYSKLAGEHARSVYANTEALAHYQAAIALGHPDIAELEE
                     AMGDLYTLLGNYEAALGSYQTAAGRSTASSQGISRMRHKMGDIYHRRGEWELAEDSFQ
                     AAAQDMKAQDDAARLSRLYADWSRTMRRRGQLERALHTASQSLDLAEKVDDDQALAQA
                     HNILGMLLRSQGNLDEAIMHLERSLLIAEKLPEPGIRIAALNNLSLAYADHGELRRAL
                     AYTQQALELCAIQGDRHRAAALHSNLADLYHSAGQEEQSMAHLKQAVVIFSEIGASET
                     ERPRPEIWKLTEW"
     gene            3222..3677
                     /locus_tag="AC16CB_16105"
     CDS             3222..3677
                     /locus_tag="AC16CB_16105"
                     /inference="COORDINATES: protein motif:HMM:NF024212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR3344047.1"
                     /translation="MPSTEQFTQVLRDWAEVFMRRSMSDFKRFIDDSGLSPSQISALM
                     RLYYGGKCGVSDIGNHLGVTNAAASQMVDRMVQLGLFTRNEDTGDRRVKQIALTSQGR
                     ELVEKGVESRRRWMEKLTTTLSPEEQDAIIGALMVLTGAARHLESEDIT"
BASE COUNT          912 a         1141 c         1087 g          763 t
ORIGIN      
        1 gctatattta tgcagtaaaa tctaagcgga ataaggccat ttgtcccaga atatcaacta
       61 caatattctg gtttcaatac cccatcactg ggtgggcacg agaacatgaa gggctgaaac
      121 agattgacga aactggctct ctacctgctg ggagctccgc tcctagagaa tgatggtcaa
      181 cttgtcgcgt ttgatacgcg caaggcgatg gccctgctgg cttacctggc tgtaaccacc
      241 aaggttcata gccgcgattc gctggccgcg ctgttctggg cagattacgc ccagtccagc
      301 gcgcgcgccg ccttgcgccg aacgctgtct gtcctcaaca aggccctgcg cgaagacacc
      361 ctggagatca cccgcgacag gttgggcttg gcgcaaggtg cagcgttatg gttcgacgtg
      421 gaagagtttc agcgcttgct caaggaacgc gaaacccacg gtcaccctca aagagagatc
      481 tgctcgcgct gcttggactg tctagagaag gccgtagatc tttatcgggg cgatttcatg
      541 gctggcttca ccctgcgcga cagccccgaa ttcgacgact ggcagtactt ccaaactgat
      601 tccctgcgac gggaactggc tggcgcccta gagcaactgg ctcacggtct ggctgcaaag
      661 gcggagtatg agcgtgccat tgtgcatgca cgccatcgcg tggcgctcga cccgctacat
      721 gaagcagccc accgccaatt gatgcagctt tacgcctggg ctggacagca atctgctgcc
      781 ctacgacaat accaagagtg cacccgcatt ctggataagg agttgggggt gccacctatg
      841 ccagagacca cccaactcta ccaagccatc aaagagcaac gcgctcagga acccctcaca
      901 acggaatatc agtttgcaga gcgcccccaa tcccttgaaa gcattgcccc tccaccttcc
      961 cgggcagcag cccacatgct tcccctggtt ggacgctggc aagagatggc atctataaag
     1021 gataattacg ccgggataca gtccgatggc tatttccaga ttctggaggg cgaagcgggt
     1081 atcggcaaaa cccgcctggc ggaggaattc ctgtccgaag cgggcacgcg cggggctttc
     1141 tctatcatga cgcgctgcta ttctggcgaa tccaatctgg cctatgcacc tttccttgaa
     1201 ggactacgag cagccttagc ccatgcccat ccaaactggc accaaccaat ccaggcgcac
     1261 tggctcgtcg aggccgcgcg cctcttgcca gagcttagcc gactgcgccc cgatttacct
     1321 gccgctccct cattagatag cccgggggca caaacgcgct tttttgaagg cgtgagccag
     1381 gttttgcagg cactttgcga cagccagcca ccaggaatct tgttcttcga cgacttgcaa
     1441 tgggcggatg aggcctcgct tgacctgctg acatacctgg tgcgccgcct gcacggacgg
     1501 cgtctgtttg tgctggctgt gtggcgcggt gaagacctgc caatggaaca tcggctgcgc
     1561 aggttgctag ccgaggcgca acgcaatggc tacgccagcg caatcacgct atctcgcctg
     1621 gatacaaaat cgatcatgga attagtgaag ttcgtagctg gagaagcggg tctcccactg
     1681 gagaacatga ctttacgcct cttcgaagag accgaaggtt tacccttctt catcacagaa
     1741 tacctggcca ccctgccgga ggtggatact gggttgcagg acgactggac tatgccgcag
     1801 ggtgtgcgcg atatccttca ctcacgcata gatgctctcg atgatgccgg gcggcagctt
     1861 ctccagaccg cggcggtgat cgggcgttcc ttcgattttg aaaccctgca gtatgccagc
     1921 gggcgaagcg aggaggaaac cactacgacc ctggaagcgc tgatcgcgag tggcttgatc
     1981 cgagaaaccc agccagggcg cgagcgccgt gccagtgacc tgctacgcac cctggattat
     2041 gacttcagcc acgataaaac gcgcgcgctc gtctatgaag aaaccagcca ggggcggcgg
     2101 cgcctgctac accagcgggt ggccgatggc ctgctacgcc attcccgcag ccggcaggga
     2161 atgcgcctgg ttgccgggca gctggcttat cactacctgc aaagcgggcg cgccaaagaa
     2221 gctgcagagt acagcaagct ggccggcgag catgctcgct cggtctatgc caacaccgaa
     2281 gccctggccc actaccaagc cgctattgcc ttaggtcacc ccgacattgc ggaactggaa
     2341 gaagccatgg gcgatctgta taccctatta ggaaattatg aggccgcact gggcagctat
     2401 caaaccgccg cgggacgctc aacagccagc agccagggta tttctcgcat gcggcacaag
     2461 atgggggata tatatcaccg gcggggtgaa tgggaattag ccgaagacag ctttcaagcc
     2521 gctgcccaag acatgaaagc ccaggacgat gcagcacgcc tttctcgcct gtatgccgat
     2581 tggagtcgaa ccatgcgacg gcgcgggcaa ctagagcggg cgctacacac ggcatcccag
     2641 tctctggatc tggccgaaaa ggtagacgac gaccaggccc tggcacaggc gcacaatatc
     2701 ttaggcatgt tgctgcgcag ccaggggaat ctggatgagg caatcatgca cctggagcgg
     2761 agcttgctga tagcagaaaa actacccgaa ccaggtatcc gcatcgcggc actcaataac
     2821 ctctccctgg cgtatgccga ccatggtgaa cttcggcgcg ccctggcata cacacagcaa
     2881 gcactggagc tgtgcgccat tcagggagac cgccaccgcg cagctgccct gcatagcaac
     2941 ctggctgacc tgtatcactc tgccgggcag gaggaacaat cgatggcgca cctcaagcaa
     3001 gctgtggtca tcttcagcga gatcggagcc agtgagaccg agcgccccag accagagatc
     3061 tggaagctga ccgagtggta aaaggggaca atagctcgcg ttttgtcgcc ccccttatcc
     3121 cccttcctct ccatcaactg cgtaaacccc tgctatattg acaaaattga cccagcagta
     3181 tatagttaag cccgtttaac taattagatg gattaaccaa tatgccttca acagagcaat
     3241 ttacacaagt attacgcgac tgggctgaag tgtttatgcg ccggtcaatg agcgacttca
     3301 agcgctttat cgatgattcc ggcctgtccc cttcccagat cagcgcctta atgcgcttat
     3361 attacggggg gaaatgcggt gtttcagaca tcggcaacca cctgggagta accaatgccg
     3421 ctgccagtca gatggtagac cgcatggtgc aacttggctt gttcacgcgc aacgaagaca
     3481 ctggtgaccg gcgggtaaag caaattgctc tcacctctca agggcgggaa ctggtagaga
     3541 agggtgtaga atcccgacgc cgttggatgg agaagcttac caccacccta agccctgagg
     3601 agcaggatgc gatcatcgga gcgctgatgg tgctaacggg agcagcccgc cacttggaat
     3661 ctgaagatat cacttaagcc catcccatag cccctttctc ttccataaga tacaggcaaa
     3721 aaagagagtc atccgaaaat cgagccaacc aaggacaagt tttttcggca gggaacgacg
     3781 ggaaaaggag aactttttgc gaaacagacg cattagcaac tcgcaacgca ctatccagcg
     3841 ccaagcgttg ggtcgggcgg tgcgttactt ggcacgctac cgaaggcagg cagtgttgcc
     3901 cta
//
LOCUS       DBNGSX010000547         2678 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1163750, whole genome shotgun sequence.
ACCESSION   DBNGSX010000547 DBNGSX010000000
VERSION     DBNGSX010000547.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1163750"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="AC16CB_16110"
     CDS             complement(<1..191)
                     /locus_tag="AC16CB_16110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344048.1"
                     /translation="MYNDVSQRNFGKARMESYLSEAQTRRLVAEYEARETKKSLPKIK
                     VPKSSFQRLLDWLFRGGESA"
     gene            complement(396..>2678)
                     /locus_tag="AC16CB_16115"
     CDS             complement(396..>2678)
                     /locus_tag="AC16CB_16115"
                     /inference="COORDINATES: protein motif:HMM:NF013124.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR3344049.1"
                     /translation="IGRQRELNEISALLEDQFTHLLTLSGPGGVGKTRLALQAAALVQ
                     DQFKDGVFFVDLAPLQDPNLVINRIAHELGVKESLTHALLNDIIDHLSTKHSLLLLDN
                     FEQILEAASTVSELIANVPSLKVLVTSREALHLYGEQEYPVLPLSLPERRADISHDRF
                     SEYEAVDLFIQRAQASNPNFRLSEINARHVAELCIHLDGLPLAIELAAARVKIFSPEY
                     MLDLLSNSLGMLTAGPRDFEARHQTLRAAIDWSYNLLDEDEKTLFARLGIFQGGHSLE
                     AIEIVCGHALESGVLHCLESLLNKSLIIQKVGPKGEPRFVMLETIQQYARERLKQSGE
                     VEQMHRRHAEYFAELAKQSEPLLRGPKQEYWSARLRADYDNLLAALEWSFAQADASIG
                     LRLAASLSEFWYYEGSISQSRKWIEVALKRVDQAPPEIHAKMLNGASMLAFVFGQHER
                     GKQLNQEALAIARQSGDRYNQACALLWLSAHATKNPNEYLESVQFSEQAVALFEELGD
                     QAGLAWGYNQIGESTRLVGDFNRAGRAYEASLAVCRQTGNRRREAISLVNLSYVAQRQ
                     GEYEKAENYVIKGLELLHSLDLRYHCAIALAMLSGPVASQGKAWRAAKLLGASQAIFE
                     RMSTGLQPADQVEIDGYAAAAAKQLDPQDYQAAWAEGQRLSFEQAMMYAPQKDLDAKR
                     GTASMSNALTVKIMGEKLSARELEVLCQLASGLSIEEIAAKLYISPSTLRSHTKNIYR
                     KLDVHRRAEAVQRGKELGLL"
BASE COUNT          551 a          748 c          711 g          668 t
ORIGIN      
        1 gcactctcgc cacctcgaaa tagccaatcg agaagtcgtt ggaagctact cttcggtacc
       61 ttgatcttgg gaagagattt tttggtttct ctggcttcgt attccgctac tagccgcctg
      121 gtttgcgctt cgcttagata ggattccatg cgagccttac caaaatttct ttgcgatacg
      181 tcgttgtaca tttggacctc cttttaacct caaacaccga ccgggatacc caatttgatg
      241 ttctgcggct tcactatcaa aaaacacttg cacgatccat atcattaaat acatggaacg
      301 ccatatgtgc cctgatcata tcgaattaat ggggacggtg tcatccccaa aattggggat
      361 gtatttgggt atttggtgct actttatttg gggggttaca gcaagcccag ttccttgccg
      421 cgttgcactg cctccgcacg ccggtgcaca tcgagttttc gatagatatt cttggtgtgg
      481 gagcgcagtg tgctaggcga gatatataat tttgcggcaa tctcttcgat ggagagacca
      541 gatgccagtt ggcatagcac ttccagctcc cgcgcgctta gcttttcccc cataattttc
      601 accgtcagcg cattgctcat ggatgctgtg ccccgctttg catccaggtc cttttgaggg
      661 gcgtacatca tggcttgctc aaaggaaagc cgttgtcctt ctgcccaggc tgcctggtaa
      721 tcttgaggat ccagctgttt ggctgcagcc gcggcatagc cgtcgatctc gacttgatcc
      781 gctggctgca ggccggtcga cattcgctca aaaatggcct gtgacgcgcc gagcagcttc
      841 gccgcccgcc aggctttgcc ttgcgaggct actgggccgg acagcatcgc cagcgcgata
      901 gcgcagtggt acctaaggtc caggctatgt aacaattcca gccctttgat gacgtaattt
      961 tccgcttttt catattcacc ctggcgctgg gccacatagc tcaggtttac cagggagatc
     1021 gcctcccggc gacggttgcc cgtctggcgg cagacagcca gtgaagcttc ataagccctg
     1081 ccagcccgat tgaaatctcc caccaggcgg gtcgactctc cgatttggtt atatccccaa
     1141 gctaagccgg cttgatcacc gagctcctcg aacagggcaa cagcctgctc agagaattga
     1201 acgctctcca ggtattcatt tgggtttttg gtggcatggg cgctgagcca taagagcgcg
     1261 cacgcctggt tgtagcggtc cccactctgt cgggcgatag ccaacgcttc ctggttcaac
     1321 tgcttgccgc gctcgtgctg cccaaaaaca aaagccaaca tactagctcc gttcagcatc
     1381 ttggcgtgaa tctctggggg tgcctgatcc acccgtttga gcgctacctc tatccatttc
     1441 cgactctgag aaatggagcc ttcgtagtac caaaattcag acaaggatgc tgccaggcgc
     1501 aacccaatgc ttgcatctgc ttgggcgaac gaccattcca gggcagccag caggttgtca
     1561 taatctgctc tgagacgtgc cgaccagtat tcctgtttgg ggcctcgcaa aagcggctcg
     1621 gattgcttgg caagttctgc aaaatattca gcatgccgcc tgtgcatttg ctccacctct
     1681 ccgctttgct tcaggcgctc gcgggcatac tgctggatgg tttccagcat gacgaagcgc
     1741 ggttcgccct tgggccctac tttctgtatg atcaggctct tattcagcag ggattctaga
     1801 caatgcagca caccgctttc caaagcgtga ccacaaacga tttcaattgc ttccaggctg
     1861 tggccgccct ggaagatacc caggcgtgca aacagcgtct tctcatcctc gtcgagcaga
     1921 ttgtaactcc agtcgatggc agctcgcagt gtctggtgtc gggcctcgaa atcgcgcggc
     1981 cccgcggtga gcatcccaag cgaattgctt agcaggtcaa gcatatattc gggcgagaat
     2041 atcttcactc tggcggctgc cagttcgatc gccaggggca ggccgtccag gtggatgcac
     2101 agttcagcca catggcgggc gttgatctcg gaaagccgga agttcgggtt agaggcctgg
     2161 gcgcgctgga tgaatagatc caccgcttca tactcagaga aacgatcatg ggagatatca
     2221 gcccgtctct ctggtaatga cagcggcagc acagggtatt cctgttcgcc atacaggtgt
     2281 agtgcctctc ggctggtcac cagaaccttc aggctgggca cgttagcgat caattcacta
     2341 accgtgctgg cagcctctag tatttgctcg aagttatcca gtaagagcag gctgtgcttt
     2401 gtgcttaggt gatctatgat gtcatttagc agagcgtgcg tcaacgactc tttgacgcct
     2461 aattcatggg cgatacggtt gatgactagg tttggatcct gcaatggtgc caggtcgacg
     2521 aagaatacgc catccttgaa ttggtcttgc accagcgcgg cagcctgaag agccaggcgc
     2581 gtcttgccca ccccgccggg accgctcaat gtcagcaggt gtgtaaactg gtcttcgagc
     2641 agagcgctaa tctcgttaag ctcgcgctgg cggccgat
//
LOCUS       DBNGSX010000548         4129 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_680099, whole genome shotgun sequence.
ACCESSION   DBNGSX010000548 DBNGSX010000000
VERSION     DBNGSX010000548.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_680099"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="AC16CB_16120"
     CDS             complement(<1..214)
                     /locus_tag="AC16CB_16120"
                     /inference="COORDINATES: protein motif:HMM:NF017206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HIR3344050.1"
                     /translation="MMILITGAAGKTGKAIVRKVAEKRGVVKAWVFRAEQIQEVEALG
                     AKEVIVGDMRDSKILRQALQNVDIIYL"
     gene            complement(260..1933)
                     /locus_tag="AC16CB_16125"
     CDS             complement(260..1933)
                     /locus_tag="AC16CB_16125"
                     /inference="COORDINATES: protein motif:HMM:NF016115.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0006569 - L-tryptophan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR3344051.1"
                     /translation="MDRDQFIQIMHSAKLYDLTQDCSIFTPPWPGEKSLEVHFFKRVT
                     GAYGGGPGANGQILNWSNTVGTHLVGETAFHSGGRSIANIPLKDLCGPGVVVDISDAV
                     SDYSLYTPEMITSRANVKKGDILIINTGYHRYSWDWPDVYNPEAQGSVESKEFGFLIR
                     HPGPSPDFFKWALDMELKLIGVDCGCAEHPMNTNIRYMHANEFKKAEAKLVADYGKTW
                     DEMFPQDEYYQMTHITLPKAHLLFVESIVGQIEELKNQRAWIMVHPIPFMEVETAWAR
                     TCALRPPEGMSEDEFFQVMQSAEMLDLTISFSVQTPQWANYVPLTVTYTKRVGGQYFG
                     MGRNGSICNSSIHLATHMDGEKHFWPAGRTIGEVPLEHWVGPGVIADISGLVSDSSVY
                     TPKMIESVVEVREGDILILKTGWHKYGWNSPDSDEFRYMIKHPGPSPDFSDWVVKKKI
                     KWLGIDAVSQDHPMNTIQRIWHPKTFEEANRKLKRDFGKDWDEMFPLDKFYQDTHLNL
                     FPKKIVHAENLGRDISTAPSGRYYIGCYLQKAMQVESMWGRFVAWKEGS"
     gene            complement(2123..3364)
                     /locus_tag="AC16CB_16130"
     CDS             complement(2123..3364)
                     /locus_tag="AC16CB_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbE family protein"
                     /protein_id="HIR3344052.1"
                     /translation="MDIEKANAETFQRMVEARPMLVGMGKAINVIPGMRDNLLMHAGP
                     PITWERASGPMQGAITGALIFEGKAKSEAEAQELVESGKIELEPCHHHQAVGPMAGII
                     SPTMSVYILENKTHGNNSYSNLNEGYGKVLRYGAYSEEVLDRLRWMEDVMAPVLGKAI
                     ELSGGIDMRALLAEALHMGDEGHNRNKAGSILFTKSLAPHIVKSTNDSDIAAKIIQFL
                     GDNALSVLNPVMAACKGMVDAAHGIEGSTIVSVMARNGTDFGIRVSGLGDQWFTAPVA
                     IPQGLYFPGFTATDASGDIGDSTITETAGIGGFAMASAPAIVTFVSGTPKDATNATLE
                     MYEICYSEHKYFTIPTLNFRGTPTGIDIRKVVETGITPRVNTGIAHKNAGVGQVGAGL
                     VRPPMAMFEQAVMAFAEHYGF"
     gene            complement(3441..>4129)
                     /locus_tag="AC16CB_16135"
     CDS             complement(3441..>4129)
                     /locus_tag="AC16CB_16135"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012061959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR3344053.1"
                     /translation="PGSITSAIQMTAKELGTHVVWPSYRRGGERGVVYNSAILIGPDG
                     EIVGIYDKTHPAPWERRDGGGWVELGTRADVFETELGTIGMIICYDGDFPELSRLLAV
                     KGAEIIIRASALQRSYDIWYITNAARAYDNHVYVVASNLVGPDARGNYGFGHSMIVNP
                     IAWRLAQARGTEEIIAAKLDPDPLRYITWGSKSLQTFDHLEDRNLKIYEEILKTARSR
                     FEPGKRFPRS"
BASE COUNT          917 a         1121 c         1005 g         1086 t
ORIGIN      
        1 taagatagat tatatccaca ttctgcaggg cttgacgcag gattttcgaa tcgcgcatgt
       61 ctccaacgat cacttctttt gcccccagcg cttcaacttc ctgaatttgc tcggcgcgaa
      121 aaacccacgc cttgacgacg cctcttttct cggcaacttt ccttactatc gccttgccgg
      181 ttttaccggc tgcacccgtg attaatatca tcataagaat aagacctggc agataaaaaa
      241 cctaccaggt cttacctgat taacttcctt ctttccaggc aacgaagcga ccccacattg
      301 actcgacttg catggctttt tggaggtagc atccgatgta gtatcgacca cttggcgctg
      361 tgctgatgtc ccttcccagg ttctcggcat ggacaatctt cttcgggaag agattgagat
      421 gggtgtcctg ataaaattta tccagaggga acatctcgtc ccaatcttta ccgaaatcgc
      481 gctttaactt tcgattggct tcctcgaatg ttttggggtg ccagatgcgt tggatggtgt
      541 tcatagggtg atcctggctg acagcatcga tgcccagcca tttgatcttc tttttaacaa
      601 cccagtctga gaagtctggt gaaggcccgg ggtgtttgat catatagcgg aactcatccg
      661 aatctgggct gttccacccg tatttgtgcc agccggtttt cagaataagg atatctcctt
      721 cgcgcacctc caccacgctc tcgatcatct tgggcgtata cacgctggag tcgctgacca
      781 accccgagat atctgcgatc acgcctggtc caacccaatg ctccaacgga acttcgccaa
      841 tcgtccgtcc ggctggccag aagtgctttt caccgtccat gtgagttgcc aggtggatgc
      901 tggagttaca tatcgagcca ttcctgccca taccgaaata ctgcccgcca actcgcttgg
      961 tataggttac cgtcagtggc acatagtttg cccactgggg tgtctgcacg ctgaacgaga
     1021 tcgtgagatc gagcatttcc gcggattgca tgacctggaa gaattcgtct tcgctcatgc
     1081 cctccggtgg gcgcaatgcg caggtgcgtg cccaggcggt ttcgacttcc atgaatggga
     1141 tggggtgaac catgatccat gcgcgttggt ttttcaactc ctcaatttga cctacgatgg
     1201 attctacaaa tagcaggtga gctttaggca atgtgatatg agtcatctgg tagtactcat
     1261 cttgtgggaa catctcatcc caggttttac cgtagtctgc taccagcttg gcctctgcct
     1321 tcttgaactc attggcgtgc atgtatcgaa tgttggtgtt catcggatgc tcagcgcagc
     1381 cgcaatctac gccgatgagc ttcaactcca tatccagcgc ccacttgaag aaatcaggtg
     1441 agggtcccgg gtgcctgatg aggaagccaa actccttgga ctccacgctt ccttgagcct
     1501 caggattgta gacatcgggc caatcccagg agtatcggtg ataacccgta ttgatgatca
     1561 ggatatcccc cttcttgaca ttggctctcg aggtgatcat ctcgggagtg taaaggctat
     1621 agtcagacac cgcatcagag atatccacga ctacgcctgg tccacataaa tccttaagtg
     1681 gaatattggc gatggatcta cccccggaat gaaaggcggt ctctccaacc aggtgtgttc
     1741 caacggtatt gctccaattc aagatctgtc cattagctcc cggtccacca ccataagcgc
     1801 cggttacgcg cttgaaaaag tggacttcga gtgatttctc accaggccaa ggcggggtga
     1861 agatgctgca atcctgggtt aagtcatata gtttggcaga atgcatgatt tggataaact
     1921 gatcgcgatc cattatttgc ctccacttct tttgtgttca tccagataaa gtaatttttg
     1981 atttctatta gctatggcgt catctagctt agaactgttg cgaccggaat gtacgcattc
     2041 gtcgcaaatc acaagacgat ggttgagaag cgctgccata atctctctgt ttgacagaat
     2101 ggataatcag gtcgcctcag ggttaaaaac cgtaatgctc agcaaaggcc atcacagcct
     2161 gctcaaacat tgccattggg gggcgtacta atcccgctcc cacctggcct acaccggcat
     2221 ttttgtgagc gatccccgta ttcacccgtg gagtgatacc agtctccacc accttgcgga
     2281 tatcaattcc ggtgggagtc ccccggaagt tcaatgtggg gatggtgaag tacttgtgtt
     2341 cagagtagca gatctcgtac atttccaggg tggcgttcgt tgcgtctttc ggtgtgccgc
     2401 tgacaaaggt aacgatagca ggcgcggatg ccatggcgaa tccaccgatc ccggctgttt
     2461 ctgtgatggt gctgtctccg atgtcaccgc tggcatctgt agctgtgaat cccggaaaat
     2521 agaggccttg cgggatggcc accggagccg tgaaccactg gtctcctaac ccgctcacgc
     2581 ggatgccgaa atcggtgccg ttgcgagcca tgacgctgac aatcgtgctc ccttcgatgc
     2641 catgcgctgc gtctaccatg cctttacagg ctgccattac tggattgaga acgctaaggg
     2701 cgttatcacc taagaattgt atgatttttg ccgctatatc gctatcgttt gttgacttga
     2761 cgatgtgtgg cgccagggat tttgtgaata aaatcgaccc ggctttattg cggttatgcc
     2821 cttcgtcacc catgtgcaac gcttcagcca gcagcgctct catatcaatc ccgccgctca
     2881 actcgatcgc tttacctaga acaggtgcca tcacatcttc catccagcgc aagcgatcca
     2941 agacttcttc gctgtaagca ccatagcgca gcactttacc atacccttcg ttcaggttgg
     3001 aataggaatt gttgccatgg gttttgttct ctaagatata cacagacatc gttggggata
     3061 taatacctgc cataggaccg acagcctggt gatggtggca aggctccagt tcaattttcc
     3121 cggattcgac cagctcttgt gcttctgcct cgctcttggc cttgccttcg aagatcaaag
     3181 cccctgtaat cgcgccctgc attggcccgg aggcgcgctc ccaggtgatc ggcggaccgg
     3241 catgcatcag cagattatcc cgcataccgg ggatgacgtt gattgctttc cccatcccca
     3301 ccagcatcgg acgggcttct accatccgtt gaaaggtctc agcattagct ttttctatgt
     3361 ccattttctg cccttttgtt ataagaacca tgcgattgtg atagagattt ggctgttgaa
     3421 ctcaacaatg ccaatctcaa ctacgatcta ggaaagcgct tcccgggctc aaatcgagat
     3481 cgagccgttt ttagaatctc ctcgtaaatt tttagattgc gatcttccag gtggtcaaag
     3541 gtttgcaagc ttttgctacc ccatgtgata tatcgaagcg gatcgggatc cagctttgca
     3601 gcgattatct cttccgtacc gcgtgcctgt gctaaccgcc aggcaatcgg attgacaatc
     3661 atgctgtgtc caaatccata attaccgcgt gcatcaggac caaccagatt gctggcgacc
     3721 acataaacgt ggttgtcata agctcgagct gcatttgtga tgtaccagat atcataactt
     3781 ctctggagag ccgaggccct gatgataatc tcagcgcctt ttacggctag cagtcgagaa
     3841 agctctggaa agtcgccgtc gtagcagatg atcataccaa tggtgcctag ctcggtttcg
     3901 aacacgtccg cccgggtgcc cagctctacc caaccaccgc catcgcggcg ctcccatggg
     3961 gcagggtgag tcttgtcata aatacccacg atctccccat caggtccgat aagaatggct
     4021 gagttataga ccacacctcg ctcacccccc cggcggtatg agggccacac cacgtgggtc
     4081 cctaactctt tggcggtcat ctggatagcg ctggtaatgc tacccggag
//
LOCUS       DBNGSX010000549         4114 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_332581, whole genome shotgun sequence.
ACCESSION   DBNGSX010000549 DBNGSX010000000
VERSION     DBNGSX010000549.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_332581"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="AC16CB_16140"
     CDS             complement(<1..837)
                     /locus_tag="AC16CB_16140"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3344054.1"
                     /translation="MYSDSANKEIVVYSTVWCPDCKRAKKFFGEQRIPYINVDIEQDT
                     EAMAYVEKVNNGKRIIPTIVFPDGSILVEPSNSELASHLGLQTKAQRQFYDVIIIGGG
                     PAGLTAATYLAREGLDTLVIEKAGLGGQVGITQVLENYPGFDEGISGGEFAERLSRQA
                     RRFGAEILQANEVLDVFPQGPYVCALTGDKIEYGARALLLATGARYRRLNIPGEDDLI
                     GTNVHFCATCDGAFYKGKKLLVVGGGNSGFEEGLFLTKFASQVDIVEFSPQVKASRIL
                     QEK"
     gene            1115..1753
                     /locus_tag="AC16CB_16145"
     CDS             1115..1753
                     /locus_tag="AC16CB_16145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3344055.1"
                     /translation="MSRRGNDQWISDLQSSGDEREAALADLHQIILRSLPYAINKWIT
                     ADDPRFQALAEEVAQETLLRVLDHLSTFEGRSQFTTWVHKIAVRVALTELRRKRWENV
                     SLDELVEGSETPPLSHLMADSSPSPDDVVEGLDVTERLQRIILEELTEKQRQAMIAIA
                     IKGMPLEEVAQRMGTNRNALYKLMHDTRLRLKRRLAHEGLTPEDVLTIFEKG"
     gene            1794..2123
                     /locus_tag="AC16CB_16150"
     CDS             1794..2123
                     /locus_tag="AC16CB_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344056.1"
                     /translation="MTGENFIKRLMRRLKIVKGPSHMDSQPAQNADELKKLMHMIEQT
                     KDVEISCDEVFALIDQYAEMAARGEDVAHLMPLVKHHLEMCADCSQEYEALMRILESP
                     EYRGGLN"
     gene            complement(2240..2686)
                     /locus_tag="AC16CB_16155"
     CDS             complement(2240..2686)
                     /locus_tag="AC16CB_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344057.1"
                     /translation="MSTTATNQIQRASLSSLAISGALAGLAGGVVFGIMMGVMGMLPM
                     VGMLVGQENAVIGSVVHLAISAFIGAVYGVIAGRLPINATNAFVGGIVNGVVWWVLGA
                     LILMPLYLGMTHMVFVIGQAQWMSLLGHILYGLVTSFAFISLAKRS"
     gene            complement(2964..3311)
                     /gene="trxA"
                     /locus_tag="AC16CB_16160"
     CDS             complement(2964..3311)
                     /gene="trxA"
                     /locus_tag="AC16CB_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008224846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR3344058.1"
                     /translation="MAAPIHVRDNEFEDVVLKAELPVVVDFWAPWCGPCRMIAPVLEE
                     IARQYADKLLVVKVNSDENYEWAINYGVHGIPTLLFVSGGQVVDRHMGALPAPVLKAK
                     TESLLNSANTVAN"
     gene            3510..>4114
                     /locus_tag="AC16CB_16165"
     CDS             3510..>4114
                     /locus_tag="AC16CB_16165"
                     /inference="COORDINATES: protein motif:HMM:NF012562.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HIR3344059.1"
                     /translation="MIQKDRKDFSAKLGVYQEGVTAALAEMQRDRIIPRIWAHDHTVW
                     KPEPVEIANRLGWLHSPEAMLETIPGLHALAEQLRAEGFRHALLLGMGGSSLAPEIFR
                     KTFGVGEGYLDLAILDSTDPDAVKDWGNRLDLSRTLFIVSTKSGGTVETFSFFKYFYQ
                     QLASHVSKERVGNHFIAITDPGSGLAEIAQKHDFRATFLNDP"
BASE COUNT         1133 a         1053 c          924 g         1004 t
ORIGIN      
        1 tttttcctgc agaattcggc tggctttcac ctgtggtgag aattccacga tatccacctg
       61 actggcaaat ttcgtgagaa acaagccttc ctcgaaacca ctgttgccgc ccccaacgac
      121 caataatttc ttacctttat agaatgcccc gtcacaggtg gcgcagaaat gcacgttagt
      181 accgatcaaa tcatcctcac ctggaatgtt caggcgccgg tacctggcgc cagtagccaa
      241 taacaacgcc cttgctccat attcaatctt atccccagtg agagcacaaa catacggacc
      301 ttgagggaaa acgtctaaaa cttcgttggc ttgcaaaatc tcagccccaa agcgacgagc
      361 ctgccggctt aaacgttctg cgaattctcc gccagagata ccttcatcga aacccggata
      421 attctccaaa acctgcgtga tcccgacctg cccgccaagg ccagcctttt caatcaccaa
      481 agtatccagt ccttctcgag ccaaataagt agccgcggtc aaaccagcag gaccgccgcc
      541 aataatgatc acatcgtaga attggcgctg cgctttcgtc tgtaatccaa gatgtgaagc
      601 taactcagaa ttggagggct caaccaggat agagccatcc ggaaaaacaa tggttggaat
      661 tatccgcttc ccgttattga ctttttccac ataagccatt gcttccgtat cctgttcgat
      721 atccacattg atatagggga tacgctgctc gccgaagaat ttcttggcgc gcttgcaatc
      781 cgggcaccag acagtgctat atacaactat ttccttattc gctgaatctg aatacatctt
      841 actcccttcc cgcatacaaa atcggatcat gacactcgcc agcaattcaa tcctgatttt
      901 cagaatcctg gatttaggaa tgaactcatg ctggctatac attgtttacc atatgactgt
      961 acctagcccc aatgacaatc gcatggtatc agcgatcttg atctccctta gtgtactacc
     1021 cttttgcata taggataatt cattcaccta tgcttttgga tagacaaccc tgtatagcag
     1081 attcttacta gggagtaatg tataatgatc atcgatgtcc agacgcggta atgaccaatg
     1141 gattagcgat ctgcaatcaa gcggcgatga gcgcgaagcc gccttggcag atttgcacca
     1201 gattatttta agaagtctac catacgccat caacaagtgg attacagctg acgatccgcg
     1261 ctttcaggct ctggctgaag aggtggctca agaaacatta ctgcgcgttc tggatcacct
     1321 gagcacattc gaaggtcgca gccaatttac gacctgggtg cacaaaatcg cggtacgggt
     1381 ggcgttaaca gaattgcgtc gaaaacgttg ggaaaatgtt tcactggatg aactggttga
     1441 aggcagtgaa accccacccc tttcccactt gatggcagat tcttcccctt ctccagatga
     1501 tgttgtcgaa ggtttagatg tcactgaacg gcttcagcgg attattctgg aggaattaac
     1561 cgaaaaacaa cgccaagcta tgatcgcgat tgccattaag ggcatgccgc tagaagaagt
     1621 cgcacaacgc atgggaacca accgcaatgc gctctacaaa cttatgcacg atacgcgctt
     1681 gcgtttgaag cgcaggttag ctcacgaggg cttgacgcca gaggatgtgc tgaccatctt
     1741 tgagaaaggg taagagaagc agtttttaag gcgtcttatt cattgggata actatgactg
     1801 gtgaaaattt tatcaaacgc ttgatgaggc gattgaaaat cgtcaagggg ccttctcaca
     1861 tggatagcca acctgcgcaa aatgcagatg agctaaaaaa gcttatgcac atgatcgagc
     1921 aaacaaagga tgtcgagatc tcatgcgatg aagtgtttgc gctcattgat caatatgccg
     1981 aaatggcggc gcggggtgaa gatgtcgccc acttgatgcc cctggtgaag caccaccttg
     2041 aaatgtgtgc ggactgttct caggaatacg aagccttgat gcgcatttta gaatctcctg
     2101 aatatcgtgg gggtttgaat tagctcgcga cgcattcatt attgtcaaca tcttaaagat
     2161 aatcgagcca accaaagctt aaacaaatag cgtaacaggg gtagatcgcc ttttccgctc
     2221 aaaacaggtt aagggttcgt caacttcttt tcgccaggga aatgaacgca aaagaagtga
     2281 ccaaaccata caggatatgt cccaacaaac tcatccactg tgcttggcca atcacaaaga
     2341 ccatgtgcgt cattcccaga tatagcggca tcagaatcaa cgcgcctaag acccaccaca
     2401 cgacaccatt gacaattcct ccaacaaaag cattggttgc attgattggc aatcgaccgg
     2461 cgataactcc atagacggcg ccgatgaagg cgctgatcgc caggtgaacg accgacccga
     2521 tcacggcatt ctcttgcccg accagcatcc cgaccatggg cagcatgccc attacgccca
     2581 tcatgatgcc gaagaccacg cctccggcaa gcccagctaa tgcacctgat attgccagac
     2641 tacttaggga agcgcgctga atttggttgg ttgcagttgt agacattgtt tctcctttac
     2701 aaaagatagt gatgatattg ggttgaaatt ccatcctgtt aattgacaac aattgtcgca
     2761 gtattcttac cttgaatgtc ttataagttt tttgatgctc tccagctcaa cctgatatcc
     2821 acccggagta aacaacaggc gacttatcta ccggttgcag aagagcaaca tactccttct
     2881 ccatcacaaa aaccccgcag gatttcctgt cgggattttt gtgtttccgt gagcggggtc
     2941 aacctggaaa tgagagagca gtttcagttc gcaacagtat tcgcggaatt aagcagcgat
     3001 tccgtcttag ctttcaacac cggggcaggc agcgctccca tatgacggtc aactacttga
     3061 cctcccgata caaacaacaa ggtcggtatg ccatgcactc cataattaat cgcccactcg
     3121 taattttcgt cgctatttac cttaaccaca agcagcttat cagcatattg tcgtgcaatt
     3181 tcttccaaaa ccggggcaat catgcggcat ggtccgcacc aaggtgccca gaaatccaca
     3241 acaactggta gttctgcttt cagtacgaca tcttcaaact cattatccct aacatgtatc
     3301 ggcgctgcca tgatataacc ttctaaacaa atctaatgcg tgagtagatg ctttgcgctg
     3361 caataagaag cagatcgatg cttgatctac atagatttag aaaagttcac ctagcgatat
     3421 cttacttcta ggataaattt gatatgatag taagaaacag gaaaacaaga tcgtctatct
     3481 agcccaagaa aattctagga aaacaagcca tgatacaaaa ggatcgcaaa gatttttctg
     3541 ccaaactggg ggtgtaccag gaaggcgtga cagcagcgtt agccgagatg cagcgcgatc
     3601 gcatcatccc ccgcatatgg gcgcatgacc acacggtctg gaaacctgag ccagtcgaga
     3661 tcgccaaccg gctaggctgg ctacacagcc cagaagccat gctggaaacc atccctggct
     3721 tgcatgccct ggcggagcaa ctgcgagctg agggctttcg ccatgcactg ctcttaggta
     3781 tgggcggttc cagcttagcg ccagaaattt tccgcaagac tttcggtgta ggagagggat
     3841 atcttgatct ggccattttg gacagcactg atccagatgc tgtgaaggac tgggggaacc
     3901 gccttgacct gagccgaacc ctgttcatcg tatcgactaa gtccggcggg acggtagaga
     3961 ctttctcttt ctttaagtat ttctaccagc aactggcgtc gcatgtaagc aaagaacggg
     4021 ttggcaatca cttcatcgcc ataactgatc caggcagcgg actggctgag attgcccaga
     4081 aacacgattt ccgcgccacc tttttaaatg atcc
//
LOCUS       DBNGSX010000550         3498 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_956896, whole genome shotgun sequence.
ACCESSION   DBNGSX010000550 DBNGSX010000000
VERSION     DBNGSX010000550.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_956896"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="AC16CB_16170"
     CDS             <1..1161
                     /locus_tag="AC16CB_16170"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3344060.1"
                     /translation="IMGRDLILMAFALTTWGIGEGAFVGFQTLYLQELGADPLRIGTI
                     IGGFGLAASLAHIPAGYLSDRLGRRPLLWVAWIIGAIAAWIMATANSLAVFVAGMMLY
                     GITAFVISPLNSYITAARGRWSVGRVLTLISATYNTGAILGPLLGGMIGERYGYQKIF
                     LFSACVFILSTLIILFIRPQPVDTPEPEEKGKNLIRNRRYLYFVFAYSLATFAMYLPQ
                     PLSPNFLQNHRGLGPAQIGQLYSISSVGIVILNLLLGQLNARIGYLIGQVGVAIFALL
                     LWRGDQFLIYGLAFFILGGFRLARSLATAHIRSIVSQSNMGLAYGLAETLTAISLLFA
                     PLLAGYLYDRDPTQIYMFSLGIIGVSVLVNSVLSAVPRTPTKIEEGYSTIET"
     gene            complement(1255..3327)
                     /gene="fusA"
                     /locus_tag="AC16CB_16175"
     CDS             complement(1255..3327)
                     /gene="fusA"
                     /locus_tag="AC16CB_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR3344061.1"
                     /translation="MKEYTTEFIRNIALASHSGSGKTMLVEAMAHFTGATTRLGKVED
                     GTTISDFEEEEQRRNLSLSTAIIPIEYNDYKINFLDTPGYTDFVGEVISALRVSDGAL
                     ILVDSVAGVEVGTEIAWNYCDTFNLPRFVVINKMNRDNANFRKAVESVQNISDSRLIS
                     VQLPWGEKLDFNGVIDLLTMKAYAGDGKNPQAIPAELIEEAEAARMELIEAAAEGEDA
                     LLEKYLEGEELTADEIKRGLRKVVRSGSFVPVLVSAGSAEIGLGPLLDAVVSLMPSPK
                     DLPPVTASGKGGDEEITPVDNGPLAAYVWKTTADPFVGKITYFRIYSGMITSDSRVWN
                     ETKSSEERLGTLHLLRGKEQIAVKVIHAGDIATVPKLTTTSTGDTLTDRGHPLTLAVP
                     EYPNALYQVAVFPKTQADSAKISPTLTRLCEEDMTLSWRQEPSTNQTILQGMGDQHID
                     VAIRKSETKFQTSLLMELPKVPYQETITKQGQAMYRHKKQTGGAGQFGEVHMRVEPLP
                     DEDYAFSNDVFGGAISSSYMPAIEKGIKAVMKEGAIAGYPIKNVKVSVIDGKEHPVDS
                     KPVAFEIAGREAFKLSVKDASPVLLEPIMNVRVTVPETNMGDVLGDLNTRRARVQGMD
                     TDKGRSVVTAQVPLAEMQRYTTDLRSITGGRGVFTMEFSHYEVVPSHLQQDIINVRQK
                     ELQTAKEE"
BASE COUNT          776 a          948 c          845 g          929 t
ORIGIN      
        1 attatgggcc gagatcttat tttgatggcc tttgccctca ccacctgggg gattggagaa
       61 ggggcgtttg tcggtttcca gacgctctat ctacaggaat tgggagcgga cccgcttcgc
      121 atcggcacga tcatcggcgg ttttggcttg gcagccagcc tagcgcatat tcctgcgggc
      181 tacttatccg atcgactagg tcgccgtccc ttactgtggg tagcctggat tattggcgcg
      241 atcgcagcct ggataatggc aaccgcaaac tcgctggcgg tgtttgtcgc tggtatgatg
      301 ctctatggta ttaccgcgtt tgttatctcc ccattgaaca gttatattac cgcggcacgc
      361 ggcaggtggt cagtggggcg cgtcctgacc ttaatttctg ccacctataa tacgggcgca
      421 attcttgggc ctctcttggg tgggatgatc ggcgagcgct atggttatca aaaaatattt
      481 ctcttttcag cctgcgtctt tatcctttca accttgatca tcctcttcat tcgtcctcag
      541 cccgtcgaca ccccggagcc cgaggaaaaa ggcaaaaacc taatcagaaa caggcgctat
      601 ctttactttg tttttgccta ttcactggcg actttcgcca tgtatctacc ccagcctctc
      661 tcgcccaact tcctacaaaa ccatcgcggt cttggtcctg cgcaaatagg gcagctatac
      721 tccatcagca gcgtggggat tgtcatactc aatttgctat tgggacaatt gaacgctcga
      781 attgggtatc tcatcggaca agttggcgtg gcgatatttg ctttgctcct ctggcgaggg
      841 gaccagtttc tcatttatgg cctagctttt tttatcctag gcggattccg tctggcgcgt
      901 tcattagcaa ccgcccacat ccgctccatt gtcagccaat ccaatatggg cttggcctac
      961 ggactagctg agacacttac tgccatctct ttgctcttcg ctccacttct agctggctat
     1021 ctttatgacc gcgatccaac gcagatttat atgttcagct tggggatcat cggcgtctcg
     1081 gtgctggtta actccgtcct gagcgctgta cctcgaaccc ctacgaagat cgaagaagga
     1141 tattcaacaa ttgaaacttg atcattagac cgcttaaaag gatttcgggg ctcaggtgca
     1201 gccccgaaat tttataatca aatgctatcg ccaaaaatct cgctaaacag gcgattactc
     1261 ttccttagcg gtttgcagtt ctttctgtcg gacattgatg atatcttgct gcaggtgcga
     1321 agggactacc tcatagtggg aaaattccat cgtaaagact ccgcgcccgc cggttatcga
     1381 gcgtagatcg gtagtgtagc gctgcatctc cgccaaaggc acttgcgccg tcacgacaga
     1441 gcgcccttta tcggtatcca tgccctggac acgcgcccta cgcgtgttca gatcacctag
     1501 aacatcaccc atattcgttt ccggtactgt gacgcgcaca ttcatgattg gctccagcag
     1561 cactggtgaa gcgtctttga ccgacagctt gaaggcttcc cgacctgcaa tctcgaacgc
     1621 cactggcttt gagtccaccg ggtgttcttt cccatcaatt acagaaacct tcacattctt
     1681 gatgggatat ccggcaatgg cgccctcttt catcactgct ttaatgcctt tttcaatcgc
     1741 aggcatatag ctgcttgaaa tggcaccccc gaagacatca ttggaaaaag cataatcctc
     1801 atcaggcaaa ggctcaacgc gcatgtgcac ctcgccaaac tggccagcgc ccccggtctg
     1861 ttttttatgg cggtacatgg cctgtccttg ctttgtgatc gtttcctggt aagggacctt
     1921 gggcaattcc atcagcaagc ttgtctgaaa ctttgtctca gatttgcgga tggccacatc
     1981 aatatgttgg tcacccatgc cttgcaggat agtctggttg gtgctgggct cctggcgcca
     2041 ggaaagtgtc atgtcctcct cgcacaagcg ggttaatgtt gggctgatct tcgcagagtc
     2101 agcttgtgtc ttggggaaga cagccacctg gtaaagcgca ttggggtatt cgggtacagc
     2161 aagggttaat ggatgccctc gatcagtgag cgtatcacca gtagaggtgg tcgttagctt
     2221 aggcactgta gcaatatcgc ccgcgtggat aaccttgact gcaatttgct ctttgccgcg
     2281 taataaatgc agggtgccca gcctttcctc gctggatttg gtttcgttcc agacccgcga
     2341 atctgaagtg atcatgcctg aatatattcg gaagtaggtt atcttgccaa cgaagggatc
     2401 tgcggtagtc ttccagacat aggctgccag cgggccgtta tcaactgggg tgatctcttc
     2461 gtcaccccct tttccgctgg ctgtcacagg tgggaggtcc ttcggggaag gcatcaaaga
     2521 gacgacagca tcgagcaacg gtcccagacc aatctccgcc gatccagcgg aaacgagaac
     2581 cgggacaaaa ctgcccgagc gaaccacctt gcgcagaccg cgcttaatct cgtcagcggt
     2641 tagctcctcg ccctctaaat atttttcgag cagggcatct tctccttctg ctgcagcttc
     2701 aatcaactcc atgcgcgccg cttccgcctc ttcaataagc tccgccggga ttgcctgagg
     2761 atttttgcca tccccagcat aggctttcat ggtcaataag tcgatcacac cattgaaatc
     2821 cagtttttcg ccccaaggta attgcaccga tatcaagcgt gaatcactga tattctgcac
     2881 cgactcgacc gccttgcgaa agttggcatt gtcgcggttc atcttattaa tcacgacaaa
     2941 gcgcggtagg ttgaatgtat cacaataatt ccaggcgatc tccgttccta cctctacccc
     3001 ggcaaccgag tcaaccagga tcagcgctcc atcggagacg cgtagcgccg agataacctc
     3061 cccaacaaag tcggtgtagc caggtgtatc caaaaaatta attttatagt cgttatactc
     3121 gatagggatg attgctgtgg acagtgaaag attgcgacgt tgctcttcct cctcaaaatc
     3181 cgagatggtc gtaccgtcct caaccttccc taggcgagtg gtcgcaccgg taaaatgcgc
     3241 cattgcttcc accagcatag tcttgccaga gccgctatgg gaagccagcg cgatattgcg
     3301 gatgaactcg gttgtatact ctttcataag ggaacccttc ctgtgagtta ttttcagaca
     3361 gcgattgatt ctaaggtata tcggctggct tgtcaaacaa accggacaaa aaaagaaggc
     3421 ggagcacaat attcactccc atccttgctt tctcgaaaca aaggagattt ttaattgtcg
     3481 aaaggaactc tgcttttc
//
LOCUS       DBNGSX010000551         3657 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_541501, whole genome shotgun sequence.
ACCESSION   DBNGSX010000551 DBNGSX010000000
VERSION     DBNGSX010000551.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_541501"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2820)
                     /locus_tag="AC16CB_16180"
     CDS             complement(<1..2820)
                     /locus_tag="AC16CB_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344062.1"
                     /translation="MSISHRGVSEKDRVVGSAENSKIELNSDATKTRPTSDRRRSLLE
                     LALITLAVLLFCAPILDLGQPTHLPGNEAEVFQILDWTLVNSLRLHGSFPIWNPNLGA
                     GQPYIADPMLHTFNPVSTLPVLLLGVRDGFKVGIALSFILAALGMWWFGAILGLSRPA
                     RIWVALMYAFAGQPVARFFQGQYLFILGFTWIPWATGNLLCLFKTGRRVYWAGTIISL
                     ALLFFSGNVYYLFYMLVFIGLIVLVYMVQLQRQRPFVRVDRKLAMALITTGALTLGVI
                     AIQLIPLSEFWPRLSKSQDVVGSHSLRQIYLDYTSKDTFRPDAFSVLPARQDYYAYIG
                     IIPISALLLLPIALWKRERKPILVLSLMLLFVVLWVNLEHMPWYEPFLRIKLLMQFSN
                     LLRVLVFGSFAILLLGALGIDTLWKMLEAQISTNPTGGWEKGRALAANLGLVLLGGIM
                     LVSAGDLYQTHRKYIETQPFDQAVYTAARWLRQHDLSDYYVRFNPTNSAHEAVISADL
                     RFINAWYHFGDIRQFDNKLNTRPLQANANYVIQGSTDPPPASPTAKLVQQIEGFNIFR
                     LPDSLPYIFSVADTLLAPENNPAPLHSSEVTTQTSFSPGPNRVEVIASASENETLVVM
                     TTHYPGWKLEVDGHSHDLLNVGGYLGTKLLPGVHKYVFYFRPRTFTIGLIVSLLCASV
                     TLYLLIIDSLPTLRQGFSQLRSTPERLRNLGHRVRRKTYAGRMVEQAIYKKGKLELEK
                     PLELDENAPIRLELETLVPQSSPSKAAWRRWKWASADLVGTLLKAITLTGLLFAGSLM
                     VYAVTRFYALERFPIYFFGDEAVQVNYAENLIARGFHGPDGALMPLFVEVSGNRWAPL
                     ISVYFHALTLTLFGKSIMVTRATSAAISLLGAGAVGLILHKVFKARYAWAGVLLVAVT
                     PAWFLHSRTAFETVLTTSFYALFLLF"
     gene            complement(2827..>3657)
                     /locus_tag="AC16CB_16185"
     CDS             complement(2827..>3657)
                     /locus_tag="AC16CB_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3344063.1"
                     /translation="YLRAVASGEAVGAYSLTEPMSGSDAGNMRSRAVREGDHYVLNGR
                     KSWVTSAPVAEYIVVFTMTEPEKKHRGITAFLIESNKPGFQQGKKEPKLGIRASATSE
                     IVFEDYKVPVENRLGEEGEGFKIAMNVLDAGRIGIAAQGLGIAEAAYEASLSYSTERE
                     AFGQKIGEFQGISFKLADMKTRIEAARLLTYNAAVAKENSKKTGERYTLEASMAKLFA
                     SETAMFVAHAALQIHGGMGYSKELPIERYFRDAKITEIYEGTSEIQRLVISRLETGLR
                     "
BASE COUNT          894 a          942 c          963 g          858 t
ORIGIN      
        1 aaacaacaga aaaagggcgt agaaggaggt ggtcaatact gtctcgaagg ctgtgcgaga
       61 gtgtaaaaac caggctgggg tcaccgccac caacagcacg cccgcccagg catagcgtgc
      121 cttgaagacc ttgtgcaata tcagcccaac tgccccggca cccagtaagc tgatagccgc
      181 cgatgtcgcc cgggtgacca tgatagactt cccgaagagg gttaaggtca gagcgtggaa
      241 ataaaccgag atcagcggtg cccagcggtt gcctgagact tccacgaata gtggcatcag
      301 ggccccatcc gggccatgaa atcccctggc aatcaggttt tcggcgtagt tcacctgtac
      361 tgcttcatcg ccaaaaaagt agattgggaa gcgctccagg gcatagaagc gtgtgacagc
      421 ataaaccatc agggaaccag caaacagcaa acctgttaat gtaatagctt ttagcagcgt
      481 gccgaccaag tctgcggagg cccatttcca gcgcctccaa gccgctttgc ttggtgagct
      541 ttgtggcacg agcgtttcga gttctaatct aatgggggca ttctcgtcga gttcgagcgg
      601 cttttctagt tctagcttgc ctttcttata tattgcctgt tccaccatac gtccggcgta
      661 agtttttcgt cgaacccggt gacccaagtt gcgtaatctc tccggggtag atctgagttg
      721 gctaaatcct tgtcgaagag ttgggagcga atcgatgatt aacaagtaga gtgtgactga
      781 tgcacagagc aggctaacga tcagcccaat agtgaatgtg cgcggcctga aataaaagac
      841 atatttatgt acaccgggca gcagcttggt tcccaggtat ccacccacat tgagcaggtc
      901 gtgagagtgc ccatctacct ccaatttcca tcctggatag tgtgtggtca tcacaaccag
      961 ggtctcgttt tctgaggcgc tggcgataac ctctacccgg tttggtccgg gtgagaagga
     1021 tgtttgagtg gtcacctcgc tgctgtgtag cggagcgggg ttattttccg gcgccagcag
     1081 ggtatccgcc acggagaaaa tgtatggcag gctatcaggc aagcgaaaga tattgaagcc
     1141 ctcgatttgt tgaactagct ttgctgtggg tgatgcgggg ggcggatcgg tggatccctg
     1201 gataacatag ttcgcattgg cttgcagggg gcgcgtgttc agcttattat caaattggcg
     1261 gatatcccca aaatggtacc aagcgttaat aaaacgcaga tctgctgata tcaccgcttc
     1321 atgtgcgctg ttggtagggt tgaagcgaac atagtaatcc gagaggtcgt gctgcctcag
     1381 ccagcgcgcc gcagtatata ccgcctgatc aaatggttgc gtctcgatat attttctatg
     1441 agtttggtaa agatcgcccg cgctcaccag cataatccca cccaagagaa ccaatcccag
     1501 gttcgcggcg agcgcgcgcc ccttctccca tcctccggtg ggatttgtgc tgatttgggc
     1561 ttccagcatt ttccaaagtg tatcaatccc aagcgcaccc agtagcagga tggcaaagct
     1621 cccaaaaacc agcacgcgca gcaggttgct aaactgcatt aacaacttga tgcgcagaaa
     1681 gggctcatac catggcatgt gctccaaatt gacccacaaa actacaaaga gcagcatcag
     1741 tgaaagcacc aaaatgggtt ttcgttctct tttccacagt gcgatgggca atagcagcag
     1801 cgctgagatt ggaatgatgc cgatatatgc atagtaatct tgtcgtgctg gtagcacgga
     1861 gaaagcatct ggtctgaatg tgtctttcga tgtgtaatct aaatagatct gtcgcagaga
     1921 atgcgagcca actacatctt gggatttgga caggcgcggc cagaactcgc tcaaaggtat
     1981 caactggatg gcgattaccc ccagcgttag cgcgccagta gtgattagtg ccatggcgag
     2041 ctttctatcg actcgcacga agggtcgctg gcgctggagt tgcaccatat acaccagtac
     2101 aatcaaaccg ataaatacca gcatatagaa caaataataa acattcccag agaagaacaa
     2161 caatgccagg gagattattg tcccagccca ataaacacgc cgcccggtct tgaataagca
     2221 aaggaggttc cctgttgccc aggggatcca ggtgaaacct aagatgaaca ggtattgtcc
     2281 ctggaaaaag cgcgccaccg gctgcccggc aaaagcatac atcaaagcta cccatatgcg
     2341 cgcagggcgg ctcaacccta ggattgctcc aaaccaccac atgcctaacg cagccagtat
     2401 aaagcttaga gcaattccaa ctttaaaccc atcacgcacc cccagcagca gcacgggaag
     2461 ggtggataca ggattgaagg tgtgcaacat tgggtcggca atgtagggtt gaccagcacc
     2521 taggttggga ttccatatag ggaaactgcc gtgcaggcgc aatgaattga ccagtgtcca
     2581 gtccaatatc tgaaagacct ctgcctcatt gccaggaaga tgtgtgggtt gtcctaaatc
     2641 taagatggga gcacaaaaga gcaagactgc taatgtgatg agtgccaact cgagaagaga
     2701 ccggcgcctg tcagacgtcg gtctggtctt tgtcgcatca ctgtttagtt caattttaga
     2761 attctcggca gagcctacta cccgatcttt ctcgctgacc ccacgatgcg agatgctcat
     2821 agtggtttat ctgagtcctg tctctagacg agaaatcaca agacgctgga tttcgctggt
     2881 gccttcgtat atttctgtga ttttggcgtc acggaagtag cgctcaatgg ggagttcttt
     2941 actgtacccc atgcctccgt gtatctgcaa cgctgcatgg gctacaaaca tggctgtttc
     3001 cgaggcaaag agcttcgcca tagacgcttc tagagtgtag cgctcgccgg tcttcttcga
     3061 gttctctttt gccacagcag cgttgtaagt aagcaagcgc gctgcttcga tacgcgtctt
     3121 catatctgcc aatttaaagg aaatgccttg gaattcgcca atcttttgcc caaaggcttc
     3181 gcgctctgtt gaataagata ggctggcttc ataggcagcc tcggcaatgc ctaacccctg
     3241 ggcagcgatg ccgatgcgtc cggcatccag tacgttcatg gcgattttga agccctcgcc
     3301 ttcttcgccc agccggtttt ctacagggac cttgtaatct tcaaagacga tctcgctggt
     3361 ggctgaggcg cggattccca atttgggttc cttcttaccc tgttgaaaac ctggcttgtt
     3421 gctctcgatg aggaatgcgg tgataccgcg gtgtttcttc tctggctcgg tcatggtaaa
     3481 gaccacgatg tactctgcca cgggcgcgct ggtgacccac gatttgcgcc cattgagcac
     3541 ataatggtca ccctcgcgta cagcccggct gcgcatattt ccagcgtctg agccggacat
     3601 cggctcggtt agcgagtaag cgcccacggc ttctccggat gctaccgcgc gcaagta
//
LOCUS       DBNGSX010000552         6840 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1648728, whole genome shotgun sequence.
ACCESSION   DBNGSX010000552 DBNGSX010000000
VERSION     DBNGSX010000552.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6840)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1648728"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2206)
                     /locus_tag="AC16CB_16190"
     CDS             complement(<1..2206)
                     /locus_tag="AC16CB_16190"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR3344064.1"
                     /translation="MKFQHWLFILLIALLASCSTSPSVTTTPSLVPTRTLAQPGVGTT
                     PVPDPGSVASAFLDAWKADDYETMYALITPLSQDAMSQESFVKHYAGIAAEMALSGID
                     YEVLSSLVEDPSSAQVGYRVIFHSSLVGDIQRETLMNLSLDQGEWRVKWDDSLVMPEL
                     AGDNFMGMERYIPARANIYDRNGHALVAQTDVTAIGLVPNQIDPDQEEKLYSWLVRLT
                     GLTGDEIVSRYASFPPGVDWYLPLGEVPAETIASNIDILSGFSGLVLGPYRARYYFDG
                     GIAPHVVGYVTTIQAGQEEEYLRKGYQRDERVGQAGLEKWGEDYLSGVRGGALYVFNP
                     QGQIITRLAEREAQPSQAIYTTLDRDFQQEVERAISGYRGAVVVLERDTGRVLAIASS
                     PSFDPNAFEPRNRNSSAMLEDIFSDPSTPTFNRAAQGQYPPGSAFKPVTMAAALESGL
                     YTPETTYQCGYFFNELAGLTLRDWTYEHYLEDGKTIPSGLLTLPEGLMRSCNPFFWHM
                     GLDLYNQDKTTAIADMARGFGLGAPTGIEVIEEEVGNVPVPQSPVDATNLAIGQGETL
                     ATPLQVAVFAAALGNGGTLYRPQLVERIAPPDGPPTFEFKPEERGKLPISPENLKVIQ
                     DAMRGVVVSTKPYGTAWHRFTGLDINVAGKTGTAESGFGDPHAWFAGYTFEDRPNQPD
                     IAAVVIVENIGEGSDYAAPIFRRVVELYFYDRPAKLYPWESGFYVTRTPTPEF"
     gene            complement(2212..2862)
                     /locus_tag="AC16CB_16195"
     CDS             complement(2212..2862)
                     /locus_tag="AC16CB_16195"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR3344065.1"
                     /translation="MLVLASNSPRRRQLMALGGWTFTLIPAAVDERPLPGEPPREYVL
                     RLAEDKARTVAGRAPQDALVIAADTAVVDGADILGKPDDAQQAETMLRRLRGRSHQVY
                     TALAVLRLTDGVLLSDLCQTEVPMRHYSDEEMAIYIASGDPFDKAGAYAIQHEEFRPV
                     EHLSGCFANVMGLPLCHLTRTLKKLGDPPERDVPTACQAALQFNCSVYPQVLTGEL"
     gene            3014..3589
                     /gene="xpt"
                     /locus_tag="AC16CB_16200"
     CDS             3014..3589
                     /gene="xpt"
                     /locus_tag="AC16CB_16200"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HIR3344066.1"
                     /translation="MEALKERILREGKNLGGGILKVDGFINHQVDPELMEACGHELAR
                     LFRDVGASKVLTAEISGIAPALSTAMHLGLPVVYARKTKPITMPDQVYLTLSPSHTKG
                     RMVELIVSPEYLAGGERVLIIDDFLASGATILGLARLAQTAGAIIVGVGTLIEKTFEG
                     GRQVLAGLGVPIEALARIRAMEGDTILFEDE"
     gene            3640..5187
                     /gene="guaA"
                     /locus_tag="AC16CB_16205"
     CDS             3640..5187
                     /gene="guaA"
                     /locus_tag="AC16CB_16205"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR3344067.1"
                     /translation="MDTIAILDYGSQYAQLIARRVREAQVYCELFPWDAPPEQVLALQ
                     PKGFILSGGPASVYEPDAPTLLPYILESDLPVLGICYGMQVLTHALGGRVAPAAQREY
                     GPALVETLKPNPLLADGDHPVWMSHGDRIETPPAGFSILARSDNSPIAAMGDLQRGYF
                     GVQFHPEVHHTPGGAEILRRFALDACHAHPEWTPESIITNSMARIRQQVGQEMVLSAV
                     SGGVDSSVATTLVSRAVGDQLVSVFVDTGLLRQGETAQVQAALHDALGAELVTVDAVD
                     QFMAALRGVTEPEQKRRIIGELFIRIFEQQARQLGMPRLLVQGTIYPDVVESSAPDRA
                     KAQRIKTHHNVGGLPAEMGFELVEPLRYLFKDEVRMVGEALGLPSSLIWRQPFPGPGL
                     AVRCLGEVTRERLERLRLADAILTEELAASGLLRMGRRGEGTAQAFAVLLPVQSVGVM
                     GDQRTYQETVALRAVTTEDFMTADWARLPYDLLARVANRIVNEVQGVNRVVYDITSKP
                     PATIEWE"
     gene            complement(5199..5417)
                     /locus_tag="AC16CB_16210"
     CDS             complement(5199..5417)
                     /locus_tag="AC16CB_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HIR3344068.1"
                     /translation="MGESVYKVIELVGTSSESWEKAATAAVEMAAKSLRDLRVAEISV
                     LDMTLENGKVRLYRAKVKVSFKYEREED"
     gene            complement(5419..5658)
                     /locus_tag="AC16CB_16215"
     CDS             complement(5419..5658)
                     /locus_tag="AC16CB_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015608263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HIR3344069.1"
                     /translation="MCRSIKTLRRPEQPPTEEELHAAALQYVRKVSGYRMPSRRNQVV
                     FEQAVEEIAMVTQKLLDSLVTTNSRLSGSKSMKGE"
     gene            complement(5677..6753)
                     /gene="asd"
                     /locus_tag="AC16CB_16220"
     CDS             complement(5677..6753)
                     /gene="asd"
                     /locus_tag="AC16CB_16220"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR3344070.1"
                     /translation="MNNQQSQPRERIPVAVLGATGAVGQRFVQLLDNHPWFKVVVLTG
                     SERAIGKTYAQACHWILSTPMPEWAREISILPAVPDDSDVPLVFSALPSSSAYEVEPY
                     YARRGVIVCSNASAFRAEPDVPLVLPEVNPEHISLISHQREKRGWTGAIVTNANCTIT
                     GLTIVLKALADQFGINKVFVVSMQAVSGAGYPGVSSMDILGNVVPYIYDEEDKIESEP
                     RKIMGTAKDDHVDLADFEITAHANRVPVLDGHMVCLSIELANKGSIDVVAEALRGFQA
                     PPISRDLPSAPRSVIELKDEPDRPQPRLDIMTGRGMTTLVGRLREDPVLDYKMVILSH
                     NTIRGAAGASIYNAELLVKQGFFE"
BASE COUNT         1506 a         2001 c         1873 g         1460 t
ORIGIN      
        1 cgaactctgg tgtaggggtg cgagtcacat agaaacccga ttcccacggg tagagcttag
       61 ctggcctatc atagaaataa agctccacca cccgccggaa gatcggcgcg gcgtaatccg
      121 agccctcacc gatgttctca acaatcacca ccgcagcgat atcaggttga tttggccgat
      181 cctcgaaggt atagcctgcg aaccaggcat gcggatcccc aaagcctgat tcagcagtgc
      241 cggttttccc agccacatta atatccaacc ccgtgaagcg gtgccaggca gtgccgtagg
      301 gctttgtgct caccaccacc ccgcgcatgg catcctggat cacctttagg ttctccgggc
      361 tgatgggcag cttgccgcgc tcctcgggct tgaactcgaa ggtgggaggt ccatcgggtg
      421 gcgcaatgcg ctcaacgagc tgtggacgat agagagtacc tccattaccc agggcagccg
      481 caaaaaccgc cacctggagt ggtgtagcca gcgtctcccc ctgaccaatc gccaggttgg
      541 tggcatccac cgggctttgc gggaccggca cattgccaac ctcctcttcg atcacctcaa
      601 tgccggtggg ggctcccaga ccaaacccac gcgccatatc cgctatggcg gtggtcttat
      661 cctggttata tagatccaga cccatgtgcc agaagaaggg attacaagag cgcatcaaac
      721 cttctggcag ggtcagcaac ccactgggga tggtcttgcc gtcctcgaga tagtgttcgt
      781 atgtccagtc cctgagcgtg aggccagcca actcattgaa gaaatagcca cactggtaag
      841 tggtctccgg agtgtacagg ccgctctcca gtgctgctgc catggtcacc ggcttgaacg
      901 ccgaaccggg gggatattgc ccctgagcgg cgcggttgaa ggtgggggtg gacgggtcgc
      961 tgaaaatatc ctccagcatg gcagacgagt tgcggtttct tggctcgaag gcgttagggt
     1021 caaaactggg cgaagaagct atggccagca cgcgcccggt gtcgcgctcc agcaccacaa
     1081 cggcaccgcg gtagcctgag atagctcgct cgacctcttg ctggaaatca cggtccagcg
     1141 tggtatagat cgcctgggag ggctgtgcct cccgctctgc cagccgggtg atgatctgcc
     1201 cctgggggtt aaaaacatac agggcgccgc cgcgcacccc cgaaaggtaa tcttctcccc
     1261 atttctccag accggcctgg cccacgcgtt catcgcgctg gtatcctttg cgcagatact
     1321 cctcttcctg acctgcttgg atggtggtga catagcccac cacatgcggt gcgatgccac
     1381 catcaaagta ataacgcgcc cggtatggcc ccagaaccag cccgctgaaa ccggaaagga
     1441 tgtcaatatt actggcgatc gtctccgcag gcacttctcc cagaggcaag taccaatcca
     1501 cgccgggggg gaagctcgcg tagcgagaga cgatctcgtc acctgtgagg cccgtcaggc
     1561 gcaccagcca gctgtagagc ttctcctcct ggtcggggtc gatctggttg ggtaccagac
     1621 caatggcggt tacatctgtt tgagccacga gagcatgccc attgcgatcg tagatattag
     1681 cgcgcgcggg gatataacgc tccataccca tgaagttatc gccagccaat tcgggcatca
     1741 ccagcgaatc atcccatttc acccgccact caccttggtc caggctcaag ttcatgagcg
     1801 tttcacgttg gatatccccc accagcgagc tgtgaaaaat cacccgatag cccacctgag
     1861 cgctactggg atcctccacc agggaggaga gaacctcgta atcgatccca ctaagcgcca
     1921 tctcggcggc aatacccgcg taatgcttta cgaaactctc ctggctcatt gcgtcctggc
     1981 taagcggagt gatcaaggca tacatggtct cgtagtcatc cgctttccaa gcatccagaa
     2041 aggcacttgc aactgagccg ggatcgggga ccggcgtggt acccacccca ggctgtgcca
     2101 gggtacgcgt gggaaccaac gaaggtgtag tagtaacact tggagaggtg ctacaactgg
     2161 caagtagagc gatcagtaaa atgaacaacc agtgttggaa tttcattgct tttacaattc
     2221 acctgtcaac acctgcgggt agacactgca attgaattgc agggctgcct ggcaggcagt
     2281 tggcacatcg cgttctggcg gatcacccaa tttcttcaaa gtgcgcgtta ggtggcataa
     2341 gggcaaaccc attacgttag caaaacaacc cgagagatgt tccaccggcc taaactcctc
     2401 gtgctggatg gcatatgccc cggccttatc gaatggatcg ccactggcga tatagattgc
     2461 catctcttca tccgagtaat gacgcatggg gacctcggtt tggcacaaat cggagagcaa
     2521 aacgccatcg gtaagacgca agactgccaa ggcggtgtat acttggtgtg agcgcccacg
     2581 caggcgtcgc agcatggttt cagcttgctg tgcatcgtcc ggtttaccca ggatatcggc
     2641 accatccacc acggctgtat ctgcggcgat cactaacgca tcctgcgggg cgcggccagc
     2701 tactgtgcgg gctttatcct cggccagtcg caacacatat tccctgggcg gttcccctgg
     2761 aagaggtcgc tcgtcaactg ctgcggggat gagcgtaaac gtccatcccc ctaatgccat
     2821 caattgcctg cggcgcggcg aattggaagc caacaccaac atcacccgat gattttatca
     2881 taatctactt ggactattat cattggggca gttcgtgaat tacccctacc gaaactacat
     2941 ccacagtaag cattaaataa cttgcattac taagagctga tcaggaaaac ccaattcacc
     3001 gcaaggagga tcgatggagg ctttgaaaga acggattctt cgcgaaggta aaaacctggg
     3061 gggcggcatt ctcaaggtgg atggattcat caaccaccag gtcgatcccg agctgatgga
     3121 ggcttgcggg catgagttgg cgcggctttt tcgtgacgtg ggcgcctcca aggtgctcac
     3181 ggctgaaatc tccggcatcg ccccagcctt gtctaccgcc atgcacctgg gtctgccggt
     3241 ggtgtatgcc cgcaagacca aacccatcac catgcccgac caggtgtacc tgacactctc
     3301 cccttcccat accaaagggc gcatggtgga gctgatcgtc tcgcccgagt atctggcagg
     3361 cggggagcgc gtcctgatta tcgatgactt cctggccagc ggcgccacta tccttggcct
     3421 agctagactg gcacagactg ccggggcgat catcgtgggg gtgggcacgc tgatcgagaa
     3481 gaccttcgag ggcgggcgtc aggtgctagc aggcctgggg gtgcccatcg aggcgctggc
     3541 gcgcatccgc gccatggaag gggatacaat cctctttgaa gacgagtgat ataaagctag
     3601 caggtctagg gtaaaaaatc ccctatcccc tgattcctta tggatactat tgccatcctt
     3661 gattacggct cacaatacgc ccaattgatc gcccggcggg tgcgcgaggc gcaggtctat
     3721 tgcgagctat tcccctggga tgcgcctccc gaacaggtgc ttgccctgca acccaaggga
     3781 tttatcctat ctggtggacc agcctcggtc tacgagccag acgcgccaac tctcttgccg
     3841 tatattttgg aaagcgacct gcccgttctt ggcatctgct atggcatgca agtactaacc
     3901 catgcgttag gggggcgcgt cgcccctgcc gcccagcgag agtatggccc agccctggta
     3961 gaaaccttga aacctaatcc actcctagcc gatggggacc acccggtgtg gatgtcacac
     4021 ggcgaccgaa tcgagacccc tcctgcgggt ttctcgatcc tggcacgcag cgataactcc
     4081 ccgattgccg ccatgggtga cctgcagcgc ggttacttcg gcgtgcagtt tcacccggag
     4141 gtccatcaca ctccaggcgg agcggagatc ttacgccgct tcgctctaga tgcctgccac
     4201 gctcacccag aatggacgcc tgaatccatc attacgaaca gcatggcgcg tattcgccag
     4261 caagtgggtc aagagatggt gctctctgct gtcagcggtg gggtggattc gagcgtggcg
     4321 acaaccttgg tgagccgcgc tgtgggggat cagcttgtct ccgtgtttgt ggatactggt
     4381 cttctgcgcc agggagaaac tgcccaggtc caggctgccc ttcacgatgc cctaggcgcg
     4441 gagctggtaa ctgtagatgc cgtcgatcaa ttcatggcag cgctacgcgg cgtgaccgaa
     4501 ccagagcaga agcgccgcat aatcggggaa ttattcatcc gcatctttga acaacaagcg
     4561 cggcagttag gcatgccgcg cctcttagtg caaggcacga tctacccaga tgtagtcgaa
     4621 tccagcgcgc cagatcgagc caaagcccag cgcattaaaa cccatcataa cgtcggcggc
     4681 ttgcctgcag agatggggtt tgagctagtg gagccgctac gctatctgtt taaagacgag
     4741 gtgcgcatgg tgggagaggc tctgggcttg ccatccagcc tgatttggcg tcagcccttc
     4801 cctggccctg gactggcagt gcgctgtctc ggcgaggtga cccgcgagcg attggagcgc
     4861 ctgaggctgg ctgacgccat cttgaccgaa gaactggccg cctctggtct actgcgcatg
     4921 gggcgtcgcg gcgaaggcac cgcccaggca ttcgctgtgc tgttaccggt gcaatcggtg
     4981 ggagtgatgg gcgaccagcg cacctatcag gagactgtgg cactaagggc tgtaacaacc
     5041 gaagatttta tgaccgcgga ctgggcgcgc ttaccctacg acctgcttgc gcgagtcgcg
     5101 aaccgtattg tcaatgaagt gcagggcgtc aaccgcgtgg tctacgatat caccagcaaa
     5161 ccaccagcga ccattgagtg ggagtaaagg gttaggtttt aatcctcttc acgctcatac
     5221 ttgaacgaca ccttcacctt ggcacgatac aagcgcactt ttccattctc aagggtcata
     5281 tcaagcactg aaatctctgc cacccgcaga tcccgcaggc tcttggcagc catctccacg
     5341 gcagcggttg cggctttctc ccacgactcg ctgctggttc ctaccaattc aatcactttg
     5401 tagacacttt cgcccatctc attctccttt catggactta ctccccgata aacgggagtt
     5461 agtggtgacc aggctgtcca gtaatttttg cgtgaccata gcgatctctt cgacagcctg
     5521 ctcgaagact acttggttgc gacgggaagg catgcggtac ccgctgacct tacgcacata
     5581 ttgtagagcc gccgcgtgca actcctcctc agtggggggc tgctccgggc ggcggagggt
     5641 tttaatactt cggcacatta cgatacctcg tcgggcttac tcaaaaaagc cctgcttgac
     5701 aagcaactct gcgttatata tggaagctcc cgccgcaccg cgaatggtgt tatgcgagag
     5761 aatcaccatc ttataatcca agacagggtc ttctctcaaa cgccccacca gggtggtcat
     5821 accacgacca gtcatgatat ccaatcgagg ctggggccga tccggctcgt ctttgagctc
     5881 aatgacagac cgcggcgcgg agggcagatc acggctaatc ggtggagctt gaaagcctct
     5941 tagcgcttca gccaccacat ctatgctccc cttgttcgcc aattcaatgc tcaggcagac
     6001 catgtgccca tccagaacag gcacgcggtt ggcatgggct gtgatttcaa agtctgccag
     6061 atccacatga tcgtccttgg cagtgcccat gattttacgc ggttccgact cgatcttgtc
     6121 ctcttcatcg tagatgtaag ggacaacatt tcccaaaatg tccattgatg aaacacccgg
     6181 atacccagca cccgagacag cctgcatgga aaccacgaag actttattaa tcccaaattg
     6241 atctgccagg gctttcaaca cgatagtcaa tccggtgatg gtgcaattgg cattggtaac
     6301 aatcgcaccc gtccagcccc ttttttctcg ctggtgagag atcaagctga tatgctccgg
     6361 attaacttca ggcaacacca gtggcacgtc gggttcagcc cgaaaggccg aggcatttga
     6421 acagacgatc acgcctcttc tcgcatagta cggttcgact tcataagcgc tcgacgatgg
     6481 taaagccgaa aacaccaggg ggacatcgga atcatcgggg accgctggca atatcgaaat
     6541 ctctcgcgcc cactcaggca ttggagtgga caggatccaa tgacaggctt gcgcataagt
     6601 cttgccgatg gcacgctcag atcccgtcaa cactacgacc ttgaaccagg gatggttatc
     6661 caatagctgg acgaaacgct gccctacggc tccggtagcg cctaacacgg cgactggaat
     6721 acgttcacga ggttgggatt gttgattatt catattcact tcgtctcatc aggtgctccc
     6781 cagatgtgat tttcgatata acaagcatcg caaccatgct gcaatttctg ttagtgtata
//
LOCUS       DBNGSX010000553         2220 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_56869, whole genome shotgun sequence.
ACCESSION   DBNGSX010000553 DBNGSX010000000
VERSION     DBNGSX010000553.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_56869"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="AC16CB_16225"
     CDS             complement(<1..585)
                     /locus_tag="AC16CB_16225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding domain-containing protein"
                     /protein_id="HIR3344071.1"
                     /translation="MRKEQDRVMIFGPVPLEAAQGLILGHNIAGPDGKRLLRKGRTLT
                     SADILVLKSIGRGSVYAARLEQGDLDENTAARRVASAVMGANTRIIGPSAGRVNLQTT
                     TLGVMRVDIDRLNEINQFEGITLATLASHTPVDPRKTIATIKIIPFALPEEVVAVVEA
                     IARHDSPIVSVNSLSEKCVSVIFSGSPSIKDRLLK"
     gene            complement(558..1001)
                     /locus_tag="AC16CB_16230"
     CDS             complement(558..1001)
                     /locus_tag="AC16CB_16230"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3344072.1"
                     /translation="MKFEGNVTINAPMQKVWEFLTNPDTVSQCAPGLKSVEILVPDQQ
                     FKAVAAIGFGAVSVLFTNDVSFVEMDPPHRAVVQVHGTAPGSAVDVMSEMNLSEGGDG
                     TTELQWTADIIIVGTIASLASRMMGGVTKKLTGMFFTCVKSKIES"
     gene            complement(1006..1989)
                     /locus_tag="AC16CB_16235"
     CDS             complement(1006..1989)
                     /locus_tag="AC16CB_16235"
                     /inference="COORDINATES: protein motif:HMM:NF024869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR3344073.1"
                     /translation="MITEHLVLINSLAEQGEPFATAVVVRAEKPTSAKPGAKALITAD
                     GTLKGWVGGSCAEPSVKKEALRALADGKPRLLRLCPPESLGTGAQEGVVEIALTCISG
                     GTYEIFIEPQLVQPRLVVVGHLATAESLVKLGRAMGYPVSVAGYEVSLERFPGAMHLQ
                     DHLDFSALQITGRTYIVVASHGNYDEEALVAALRTPAPYVSLVASPKRAQAALQYLRD
                     SDLPAGWQERFKFPAGLDFGAVTTDEIALSILAEIVQIHRRRTGQQIQQLLQIESAPV
                     SETAIDPVCGMTVEIATARYSLEHQGSMYYFCAAGCRHKFERNPENYLMKE"
BASE COUNT          466 a          636 c          611 g          507 t
ORIGIN      
        1 cttgagcagc ctgtctttta tcgaagggga tccggaaaaa atcacgctca cacatttttc
       61 tgagagactg ttcacggata caatggggct atcatggcga gcgattgcct cgacaaccgc
      121 caccacctct tctggaagtg caaaggggat gatcttgatc gttgcgatag tttttctggg
      181 gtcaacgggg gtgtggctgg ccagggtggc aagcgtgatc ccttcaaatt ggttgatctc
      241 gttgagccgg tcgatatcca cacgcatcac gcctaaggtc gttgtttgca ggttcacccg
      301 accagccgaa ggaccaatga tcctggtgtt ggcacccatc acagccgatg cgacccgccg
      361 ggcggctgtg ttttcatcca ggtctccttg ctccagacgg gctgcataca cactgcctct
      421 gccgatggac ttcagcacca ggatgtcggc ggaggtaagt gtccgccctt tccgcagcag
      481 ccttttgccg tctggaccgg cgatgttatg acccaggatg agcccttggg cagcctccaa
      541 tgggaccgga ccaaatatca tgactcgatc ttgctcttta cgcatgtgaa gaacatgcca
      601 gttaatttct tggtaacgcc tcccatcatc cgtgaagcca ggctggcaat ggtgccaacg
      661 atgatgatat ccgccgtcca ttgcagctct gtggtcccat ctcctccttc agatagattc
      721 atctcgctca tcacgtctac cgcactgcca ggagctgtac cgtggacctg gacaacagcg
      781 cgatgggggg ggtccatctc aacaaaagac acatcattcg taaagaggac agaaaccgcc
      841 ccaaagccaa tggcagctac cgccttaaac tgctgatcgg ggacgaggat ctccaccgat
      901 ttcaacccgg gagcacactg gctgaccgtg tcgggattgg tcaggaactc ccagaccttc
      961 tgcattgggg cattgatggt tacgttacct tcgaatttca cgatattact ccttcatgag
     1021 atagttttca gggttgcgtt cgaacttgtg gcggcatccg gctgcgcaga agtagtacat
     1081 gctgccctgg tgttccaagg aatagcgggc agttgcaatt tcaactgtca tcccgcatac
     1141 cgggtcgatc gctgtttcgg aaacaggtgc gctctcaatt tgcagcagtt gttggatttg
     1201 ctgccctgtc ctgcggcggt ggatctggac tatctcagcc aagatgctca gggcgatctc
     1261 atcagttgtg acagctccga aatcgagccc agcggggaac ttaaagcgct cttgccagcc
     1321 cgcggggaga tcagaatccc gcagatattg caaggcagcc tgcgcccttt tggggcttgc
     1381 taccaagctg acataaggcg cgggagttcg caaggcagca accaaggctt cctcatcgta
     1441 gttgccgtgg ctggcgacca cgatatatgt gcgccctgtg atctgcagcg cggaaaaatc
     1501 gagatgatcc tgaaggtgca tggcgcctgg gaagcgttcc aatgagactt cgtatccagc
     1561 tacggaaacc gggtagccca tcgccctgcc gagcttgacc agggattcag ctgtcgctaa
     1621 gtgccccaca accaccaacc ggggctgaac gagctggggt tctatgaaga tctcataggt
     1681 tcccccgctg atgcaggtta gtgcgatctc gaccacgcct tcctgtgcac ccgttccaag
     1741 agattccggc gggcacaggc gcagcaatct gggcttacca tcagcaagcg cacgcagggc
     1801 ttctttcttg accgatggct ccgcacagct tccgccgacc cagcccttca gcgtgccatc
     1861 cgcagttatc agcgctttgg cgcccggttt tgcagaggtt ggcttttccg cacgcacgac
     1921 gaccgcagtt gcaaagggct caccctgctc ggcaagagaa tttatgagta cgaggtgttc
     1981 tgtgatcata actggagcct gtaatccccc ggggtgctca ggcaccccgg gggatgattt
     2041 attgagagtt atttatattc tgctgcccca tgctcgttga ggagctccca aacccgggag
     2101 ggcttgatgg gaatatccac gtggcgaacc ccaatatgcc agagcgcatc taccacagcg
     2161 tttacaatcg cggcgggggc gcctaccgtt gcagattctc ccacgccttt tgccccgatg
//
LOCUS       DBNGSX010000554        10122 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1095459, whole genome shotgun sequence.
ACCESSION   DBNGSX010000554 DBNGSX010000000
VERSION     DBNGSX010000554.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10122)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10122)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10122
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1095459"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /locus_tag="AC16CB_16240"
     CDS             <1..1306
                     /locus_tag="AC16CB_16240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LeuD/DmdB family oxidoreductase small subunit"
                     /protein_id="HIR3344074.1"
                     /translation="LIDQKKITVHSGVPTMFALELNHPSFKPSMCASLRTGIMSGAAC
                     PPELVKRVRAEMGLNLILAYGLTEASPSVAMTRLDDGPVTTTQTVGRPMEGVEIKVID
                     PDGNTLPPGETGELCVRGYNIMLGYWDEPEETSRVLDKDGWLHTGDQAIIDPNGPVRI
                     LGRMDEVINRGGFKIYPGSVEMVLRAYPGVKEATVIGVPDLIFGELTVACLVAQPGLE
                     ISSDELLAFLADHLADYALPDRIIFFDALPRRGSGLVRRNYLRERVRIRGRVWKFGKN
                     IDTDAIIPARRCNTAIPSELALYCMEDADPHFVQKMKRGDLIVADTNFGCGSSREVAP
                     LTIKAAGISAVIAKSFARIFFRNAINIGLPILECPKAVDSIREGDEVEVEPTAGLIKN
                     LTRNEVYQAEPFPDFLKSIIDKGGLLAYVEEQLTLSKTGENQ"
     gene            1303..2451
                     /locus_tag="AC16CB_16245"
     CDS             1303..2451
                     /locus_tag="AC16CB_16245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3344075.1"
                     /translation="MIDFELTDEQREMREMAHKFAEKEIRPIAAEYDEREEVPWEVIQ
                     KATKAGFMSYFIPERYGGGGITDLFTHCLVDEEIFWGCAGIATILGGTSLCATPILLA
                     GTEEQKEKYLSRFCDLDQPKLGAFALTEPSAGSDPASMITQAHRDGKYYVLNGYKTFI
                     TNAGIADVYVIFATVDVSRGVRGVTAFIVDKDWEGVVPGKKEKKMGIRASHTATLALE
                     GVRVPVENRLGEEGEGFAIAMRTFEHTRTHIAIGAVGLARAAFEYALQYAQERVQFGK
                     PISHHQAVSFMLAEMATQIDAARLLAWRAAYLADQGVPCTKEASMAKAFAADMAMQAT
                     TDAVQILGGYGYMREYPVEKWMRDAKIMQIYEGTAQIQRMIIARFITN"
     gene            2470..3549
                     /locus_tag="AC16CB_16250"
     CDS             2470..3549
                     /locus_tag="AC16CB_16250"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR3344076.1"
                     /translation="MKTYNIGVIAGDGIGPEVAAEGLKVLRTVSAQEGFECNLVDYPY
                     SGTYYLKTKELVPERVIDEWRTLDAVYLGAIGHPDVEPGLVERSVILGLRFGLDLYIN
                     LRPIKLYADHLCPLKDKGPKDIDFVVVRENTEGLYSQIGGFLKKGTPDEVATVTGVYT
                     RKGVERAIRYAFELARQQGKARADQRQPRLTLVDKSNAIRPQDLWVRTFEEVGQEYPD
                     VEQDHAYVDACTMWMVKNPEWFDVIVTTNLFGDIITDLGAMLQGGMGIAASGNIHPGK
                     TSMFEPIHGSAPKYTGKNIACPLGAIMAVSMMLDFLGEKKAALSVESAVAHLLASGAI
                     PSADARSGLSTTQIGDMVVESLQKA"
     gene            3666..5180
                     /gene="accC"
                     /locus_tag="AC16CB_16255"
     CDS             3666..5180
                     /gene="accC"
                     /locus_tag="AC16CB_16255"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR3344077.1"
                     /translation="MFNKVLIANRGEIAVRIIRACRELGMRTVAVYSEADRQGLHVRY
                     ADEAYLLGPAPSRESYLRSDKIIEIARKCGAKAIHPGYGFLAERADFAQDCLDVGLAF
                     IGPKPSSIAAMGDKAVARATVAKAGVAVVPGTEGEGALGDEELLSLAPEIGFPLLIKA
                     TAGGGGKGMRVVHHLEEMHSLLHSARREAEASFGDGNVYLEKLVEGARHIEFQILADT
                     HGNVIHLGERECSLQRRHQKLLEESPSPFISDDDEMRQRMGNVAVRAAQAVGYVNAGT
                     IEFLVDKDKNFYFLEMNTRLQVEHPVTEMVTGVDIVKEQIRIARGRQMRYSQENINLN
                     GWAIECRINAEDAYNNFLPSTGQISHSLLPTGPGVRVDTGVYPGFEISPYYDSLISKL
                     IVWGETRAEAILRMRRALEEYKIVGVRTNIPFHQNLMDSHRFMGGQFDTRFVEERFSM
                     EAAEEGKSPRPEIAAIIATLVAHEQTQRAAHIIQRGERDTSNWKWISRWERMQR"
     gene            5208..5705
                     /locus_tag="AC16CB_16260"
     CDS             5208..5705
                     /locus_tag="AC16CB_16260"
                     /inference="COORDINATES: protein motif:HMM:NF012584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016421 - CoA carboxylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HIR3344078.1"
                     /translation="MKYVTTIGEREYIIEIVDERHIILDGTPFEVDFDSVSGQPVYSL
                     LLDGQSYESFVYSSEDGWEVLLHGTLYPASVEDEREKRLRAAAGGNIAEAGEFYLRAP
                     MPGLVISILVDEGQSVEKGDVLVILESMKMQNELKSPRPGTIARMRVKAGDSVEQKQT
                     LLSVV"
     gene            5843..6463
                     /locus_tag="AC16CB_16265"
     CDS             5843..6463
                     /locus_tag="AC16CB_16265"
                     /inference="COORDINATES: protein motif:HMM:NF014038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HIR3344079.1"
                     /translation="MKNQELEVKFYVADLLEIEARLRALSAHLEQSRVHEVNLRFDTP
                     DGDLGRGYRVLRLRQDDAARLTYKGPSQYQEGVRARQEIEFTVSDFEAARAFLEALGY
                     VVSMMYEKYRTTYELDGVHITLDEMPYGDFIEIEGPDPKSIRSIQDRLELDWEKRVPE
                     SYTALFEQLRLALRLPFKDLSFANFDGLDISMSVIRIQPADLNEQR"
     gene            6502..7086
                     /locus_tag="AC16CB_16270"
     CDS             6502..7086
                     /locus_tag="AC16CB_16270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR3344080.1"
                     /translation="MAKDFSLNVTPPNKLQRAEYACDDDWIRNFFLQAEVGHVATSWD
                     EQPFITPILFWYDPQHHEIYFHTNITGRLRANIEHQDKVCIEASRIGRALPANTALEF
                     ALQYESAVAFGRGRLILDDDEKRRTLYGLIEKYFPKMTAGVHYRPITDQELKRTAVYA
                     IAIESWSGKRNWPARAKQLPDWPPLGDEWFEEDN"
     gene            7083..8033
                     /locus_tag="AC16CB_16275"
     CDS             7083..8033
                     /locus_tag="AC16CB_16275"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3344081.1"
                     /translation="MNEAAVLSEPSSRLARGYLICLTATVIWSTTGIFIGYITQNFNM
                     PPLLLAFWRDLIVSATLLTTFTLLSRSMLRISKNNLAFLAWYGLALTFLNTSWTISVA
                     LNGAAVSTVLVYSSPAITALVGWRLYGESLDMAKISAIVLSILGAALVSGAYQPQVWQ
                     VNLIGVIAGLLSGVAMAMYSLMGKGASLRQINPWSTLSYTFASATLFLLIINLTSLGQ
                     AQGLSHSNLLWLGNTPTPWIVLLILAIGPTIGGYGLYMVSLGYLQASVANLIATLEPV
                     FTAILAYLLLAERLNSVQILGGILIFTGVVLLRMYEGRRK"
     gene            8030..9457
                     /locus_tag="AC16CB_16280"
     CDS             8030..9457
                     /locus_tag="AC16CB_16280"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020976.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HIR3344082.1"
                     /translation="MTELLLGPVIGGLTHDSAYLWGRADGPSVLHAWLGRNPDLSDAR
                     LAGTSLPLSSDTGFAGVAVTKELQPNSRYYYALTLSGSPPSPATEGFSSFTTAPSPGE
                     RLSFSFAFGSCFRPENADGGRIFDAIEEQRKADDLRFILLLGDQIYADAYQHNGIGKI
                     ACTLQEYRDVYAYTWSRPPLQRLLANLPAYMTLDDHEVDDDWRWVDSNRRWANIPWWD
                     KVSRWLKGRPLQERRLPVKRAQDALQAYWEHQGMHAPSYKLTPQLNQAGQYELDVKDP
                     GSLAYTFTFGAAAFFVLDTRSMRVRNSREKIMLGEGQWQALKTWLRAVKDDYPIKFLV
                     TSCSMLYPMWHDLPRDRWTGYPEERDRLLHFLAANGIDGVYLLAGDLHSSHAVYAELY
                     GPEGRSLPLWEFCSSPFEQDCNRWGRYTYRPLHTSPVKVSYRAFSVHQVNYGVVKVDF
                     SRKDRPNVTFQIHDQQGKKLEAISV"
     gene            complement(9461..>10122)
                     /locus_tag="AC16CB_16285"
     CDS             complement(9461..>10122)
                     /locus_tag="AC16CB_16285"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HIR3344083.1"
                     /translation="FSAAITRYLLSQGAKLVVVACNAASAAALQYLRQTFPQVPFVGM
                     EPAVKPAAEGTHSGVVGVLATPATFQGALYASVVERFAQGVTLLQHTCPGLVAQIEAG
                     NLDSSETRRILEDALHPMLLRGLDTVVLGCTHYPFVIPLIQEIVGTSVRVVDPAPAVA
                     RQVGRVLEARGLHHPDREEGEVIYLTTGDPEQLEALLPRLVGEEGLVDRLEWGREGDL
                     L"
BASE COUNT         2329 a         2778 c         2875 g         2140 t
ORIGIN      
        1 cttgatcgat caaaagaaga tcacggtcca tagcggtgtg cccaccatgt ttgccctaga
       61 gctgaaccac cccagcttta agcccagcat gtgcgccagc ctgcgcactg gcatcatgtc
      121 tggtgcggct tgtccacccg aattggtcaa gcgggtgcgc gccgagatgg gtttgaattt
      181 gattctggcg tatgggttaa ccgaggcttc tccatcagtt gccatgacac gccttgatga
      241 tggcccggta accaccaccc aaaccgtggg tcgccccatg gaaggcgtgg agatcaaagt
      301 gatcgatccg gatggcaaca cgctgcctcc aggcgagaca ggcgagctgt gtgtgcgcgg
      361 ctacaacatt atgctgggat attgggacga accagaagaa acttcccgcg tgctcgataa
      421 agatggctgg ttgcacaccg gcgaccaggc aatcatcgat cccaacggcc cggtgcgcat
      481 cttgggacgc atggatgaag tcatcaatcg aggcgggttc aaaatctatc cggggtcggt
      541 cgagatggtg ctgcgcgcct atcctggggt caaggaagct accgtcatcg gggtgccaga
      601 tttaatcttt ggtgaattaa cggtggcttg tcttgttgcc caacccggac ttgagatcag
      661 tagcgatgag ttattagcct ttttagccga ccatctggca gattatgcct tgcccgaccg
      721 gatcatcttc ttcgatgcgc tcccccgccg ggggagtgga ttggtgagaa gaaattatct
      781 gcgagaaagg gtgcgcatac gtgggcgggt ctggaaattt ggaaaaaata ttgatactga
      841 tgcgattatc cctgcccggc gctgcaacac agccatcccc agcgagctgg ccttgtactg
      901 catggaagac gctgatcccc acttcgtgca gaagatgaaa cgcggtgatc tgatcgtcgc
      961 tgacacgaat tttgggtgcg gatcttcgcg cgaggtggcg cctctgacca tcaaagccgc
     1021 ggggatctcg gcagtgatcg cgaaatcttt cgcccgcatt ttcttccgca acgccatcaa
     1081 catcggcctg ccgatcctgg aatgccctaa ggcggtcgac agcatccgag aaggcgacga
     1141 ggtggaagtt gagcccacgg caggcttgat caagaacctg acccgcaacg aggtctatca
     1201 agcggagccc ttccctgatt ttctaaaaag catcattgac aaaggcggtc tgctcgctta
     1261 tgtggaggag cagttgactc tgagcaaaac gggagaaaat caatgatcga ttttgagctg
     1321 accgacgaac aacgcgaaat gcgcgagatg gcgcacaaat ttgcggaaaa ggagattcgc
     1381 cccatcgccg cagaatatga cgagcgagaa gaagtgcctt gggaggttat tcagaaagcg
     1441 accaaggccg gcttcatgtc ctactttatc ccagagcgtt atggcggagg ggggattacg
     1501 gatttgttca cccactgtct ggtggatgag gagatctttt ggggctgcgc ggggattgcc
     1561 actatcctgg gaggcaccag tctgtgtgcc acacccattc tcctcgctgg cactgaagag
     1621 caaaaggaaa agtatctatc tcgtttttgc gatcttgacc agccaaaact tggagcattt
     1681 gccctgaccg agccatccgc aggctctgat ccagccagca tgatcacgca agctcaccgg
     1741 gacggcaaat attacgtcct gaacggctat aaaaccttca tcaccaatgc tgggatcgct
     1801 gatgtctatg tcatttttgc caccgtagat gtcagccgtg gcgtgcgagg tgtaaccgcc
     1861 ttcatcgttg acaaagactg ggaaggggta gtgccaggga aaaaagagaa gaagatgggc
     1921 atccgcgctt cccacacagc caccctggcg ttggaaggtg tgcgcgtgcc ggtcgaaaac
     1981 cgtctgggag aggagggtga ggggtttgct attgctatgc ggaccttcga acatacccgc
     2041 acccacatcg caatcggggc agtggggctt gcacgagccg cttttgaata tgccctgcag
     2101 tatgctcagg aaagggtgca gttcggcaaa ccgatcagcc accatcaagc ggtgtcattc
     2161 atgctagccg aaatggcaac ccaaatcgac gccgcccggc tgctcgcctg gcgggcagcc
     2221 tatctggcag atcagggcgt gccgtgcacc aaggaagcca gcatggcgaa ggccttcgcc
     2281 gcagatatgg caatgcaggc aaccaccgat gcagtccaaa tcctgggtgg gtacgggtac
     2341 atgcgcgagt atccggtcga gaaatggatg cgtgatgcca agatcatgca aatctatgag
     2401 ggcacagcgc agattcaacg catgatcatc gctcgtttta taaccaacta aatgaataat
     2461 ggagaggaca tgaaaacata caacataggc gtaatagcag gtgatggcat cggcccggaa
     2521 gtggcggcag aaggactcaa agtactccgc acggttagcg cccaagaggg ctttgagtgc
     2581 aatttggtgg attatccata ttcgggtact tattacctga agacgaagga gttagtccct
     2641 gagcgcgtca tcgacgagtg gcggacactc gatgcggtct atctgggtgc cattgggcac
     2701 ccggatgtgg agcccggcct ggtagaacgc tcggtaatct taggcctgcg ctttggcctg
     2761 gacctctaca ttaacctgcg ccccatcaag ctctacgccg atcatttgtg ccccttgaag
     2821 gataaaggac ccaaagatat cgactttgtg gtcgtgcgag agaataccga aggcttgtac
     2881 tcccaaatcg gcggattctt gaaaaaaggc acccctgacg aggtggcgac ggtcacggga
     2941 gtgtacactc gcaagggcgt tgaacgggcg atacgttatg ccttcgagct cgcccggcaa
     3001 caggggaagg cacgcgcaga tcagaggcag ccgcgcctga ccttggtgga taaatctaac
     3061 gccatccggc cgcaggattt atgggtgcgt acgttcgaag aagtcgggca ggaatatccg
     3121 gatgtggaac aggatcatgc ctacgtggac gcctgcacca tgtggatggt gaaaaacccc
     3181 gagtggtttg acgtcatcgt aactaccaac ctgtttggag acatcatcac ggacctgggc
     3241 gcaatgctgc agggcggcat gggtatcgcc gcttcaggta atatccatcc cggcaaaact
     3301 tccatgttcg agccgatcca cggctctgcc cccaagtaca ctggcaagaa catcgcttgc
     3361 cctttaggtg ctattatggc agtgagcatg atgttggatt tcctcgggga aaagaaggct
     3421 gcgctaagcg tggagtcggc tgtggcccat ctccttgcca gcggagctat tccttcggcc
     3481 gatgcacgca gcggattgag cacaacccag attggcgata tggtggtcga atcgctgcag
     3541 aaagcctaac cagggaaagt gtcggcctat accaggcttg ttgaaaaccc gatctgatgg
     3601 taggataact acctggtttg acatccagct cattatccct agaccaacaa aagccatgaa
     3661 gaaccatgtt caataaagtc ctcatcgcga accggggcga aattgctgta cgcatcatcc
     3721 gcgcttgccg ggagctaggt atgcgaacag tggcagtgta ttccgaggcg gatcgccagg
     3781 ggctacatgt gcgctatgcc gacgaggcat atctactggg accggccccc tcgcgcgagt
     3841 cttatcttcg cagcgataag atcattgaga ttgcgcgcaa atgcggcgca aaggcaatcc
     3901 accccgggta tggcttttta gccgagcgag ccgactttgc ccaagactgc cttgacgtcg
     3961 ggttggcatt catcggcccg aagccttcat ctatcgctgc catgggcgat aaagccgtgg
     4021 cacgtgctac cgtggcaaag gctggcgtgg ctgttgtacc aggcaccgaa ggcgaaggcg
     4081 cgttgggcga tgaggagctg ctgtctttgg ctccagaaat cggtttcccc ttgctaatca
     4141 aagccacggc gggcggtggc ggcaaaggca tgcgcgtggt gcatcatcta gaggaaatgc
     4201 actcactatt gcactccgca cggcgggagg cagaggccag ctttggggat ggcaatgttt
     4261 acttggaaaa gctggttgaa ggagcgcgac acatcgaatt tcagatcctg gcggataccc
     4321 acggcaatgt gattcacctg ggagaacgcg agtgttccct gcagcgccgc caccagaaac
     4381 tattggagga atcgccctcc cccttcatca gtgatgatga cgagatgcgc cagcgcatgg
     4441 gcaatgtggc ggtgcgcgct gcgcaggcag taggctatgt caacgccgga accatcgaat
     4501 tcctggtaga taaggataaa aatttttact tcctggagat gaacacccgc ctgcaagtgg
     4561 agcatccggt cactgaaatg gtcactggtg tggacattgt caaggaacaa atccgcatcg
     4621 ctcgcgggcg gcaaatgcgc tattcccagg agaatatcaa tctcaacggt tgggctatcg
     4681 agtgtcgcat caatgccgag gatgcatata ataacttcct gccatccacc ggtcagattt
     4741 ctcacagcct gctgcccacc gggccgggtg tgcgcgtgga taccggggtc tatcccggat
     4801 ttgaaatatc accctattat gactcgctga tctcaaaatt gattgtatgg ggagaaacgc
     4861 gcgccgaggc gatattacgc atgcgccgtg ccctggaaga gtacaagatc gtcggcgtgc
     4921 gcaccaatat tcccttccac caaaatctaa tggattcgca tcgcttcatg ggcgggcaat
     4981 tcgacacgcg ttttgttgaa gagcgctttt ctatggaagc agccgaggag ggcaaatccc
     5041 ctcgcccgga gatcgctgcc atcatcgcca ctctggtagc ccacgagcag acccaacgcg
     5101 ctgcccacat tatccagcgc ggagagcggg ataccagcaa ctggaaatgg atcagccgct
     5161 gggaacgtat gcagagatga gtcaggtgtg aggagtatgg ggtatttatg aaatacgtga
     5221 ccacgatcgg ggaacgcgag tacatcatcg aaatcgtcga cgagcgccat atcatcctgg
     5281 atggaacccc cttcgaggtg gacttcgatt cagtcagcgg gcaaccggtt tattccctgc
     5341 tgctggatgg acaatcgtat gagtccttcg tctactccag tgaagatggc tgggaagtat
     5401 tgctgcatgg cacgctttac ccagcttctg tggaagatga acgtgagaag cgcttgcgcg
     5461 ccgccgcagg aggcaacatc gccgaggccg gagaattcta cttgcgggca cctatgccag
     5521 gcttggtgat ctccatattg gtcgatgagg gtcagagcgt ggaaaagggt gacgtgctgg
     5581 tcattctaga atcgatgaag atgcagaacg aactcaagtc cccccgccca ggcacgattg
     5641 cgcgcatgcg cgtaaaagcg ggagatagtg tggaacaaaa gcagaccctg ttgagcgtcg
     5701 tctgaaaacc agaaacgaaa tgtaactcac cccaatccct tttgggcaga accctgcgtt
     5761 gcctggaagt cggggcaaaa ctttcccgca gtctcgggaa ggatgcaaac caaacggcgg
     5821 ggatgacggg cagggaccaa agatgaaaaa ccaggaattg gaagtaaagt tctatgtcgc
     5881 cgatctattg gagatcgagg cgcgcttgcg cgccttaagt gcgcacctcg agcaatcgcg
     5941 cgtgcacgag gtcaatttgc gattcgatac acccgatggc gatctggggc gcgggtaccg
     6001 cgtgctgcgc ttgaggcagg atgatgccgc caggctgacc tataaaggtc ccagccagta
     6061 ccaggagggt gtgcgcgccc gccaggagat cgagttcacc gtcagcgact tcgaggctgc
     6121 acgcgccttc ctggaggcgc tgggttatgt tgtgtccatg atgtacgaga agtaccgcac
     6181 cacatacgaa ctggacggtg tgcatatcac cctggatgag atgccttacg gcgatttcat
     6241 cgagatcgag ggaccagatc caaaaagtat acgaagcatc caagaccgcc tcgagctaga
     6301 ttgggagaag cgcgtccctg agagctacac ggcgctcttt gagcaactgc gcctcgccct
     6361 gagactgccc ttcaaagacc tgagcttcgc caacttcgac ggacttgata tctcgatgag
     6421 tgttatcaga atacaacctg cagacctgaa cgaacaaaga taagcacgta gacccataca
     6481 acacaactca ggatatattt catggccaaa gatttcagcc tcaatgtcac tcccccaaac
     6541 aagttacagc gagccgaata cgcctgcgat gacgattgga tccgcaactt cttcctgcaa
     6601 gcggaagttg ggcacgtcgc cacaagctgg gatgagcagc cttttatcac gcctatccta
     6661 ttctggtatg acccgcaaca ccacgagatt tacttccata caaatatcac cgggcgactg
     6721 cgcgccaaca ttgagcacca ggataaagtt tgcattgaag ccagccgcat aggccgtgcg
     6781 ctgcccgcca acacggcact ggaatttgcc ttgcagtacg agagtgcggt cgcctttggg
     6841 cggggacgct tgattttaga tgacgacgaa aagcggcgca ccctgtatgg gttaatagaa
     6901 aaatacttcc caaaaatgac cgcaggagtg cactaccgac ccatcacaga ccaggaattg
     6961 aaacggacgg cagtttatgc catcgccatc gagagctgga gcggcaagcg caactggccg
     7021 gcgcgagcca aacaactccc tgactggccc ccgcttgggg atgaatggtt cgaggaggac
     7081 aattgaacga ggccgccgtt ctaagcgaac cgagctcccg cctggcgcgg gggtacctga
     7141 tctgcctgac agccaccgta atctggtcga ccaccggcat attcatcggc tacattactc
     7201 agaacttcaa tatgccgccg ctcctgctgg cattttggcg cgacctgatt gtaagcgcca
     7261 ctttattgac tacgttcacc ctcctcagcc gttctatgct gcgcatatcg aaaaacaacc
     7321 tggcgtttct agcgtggtat ggactggcgc ttaccttcct gaatacttct tggaccatct
     7381 cggtggctct gaatggcgcg gcggtttcca ccgtgctggt ctatagctcc ccggcaatta
     7441 cagccctggt agggtggcgt ttgtacggcg agagcctgga tatggccaaa atatccgcca
     7501 ttgttctgag tattttaggg gctgccctag tctcaggcgc ataccagccg caggtctggc
     7561 aggtcaactt gatcggtgtg atagccgggt tgctctcggg agtggctatg gcaatgtata
     7621 gcttgatggg caaaggggct tccctgcgcc agatcaaccc ctggtcgacc ctttcgtaca
     7681 ccttcgccag tgccacactt tttctgttga tcattaattt aacttcattg ggacaagcgc
     7741 aaggcttgtc ccactcaaac ctgctctggt tggggaatac tcccaccccg tggattgtgc
     7801 tcctaatcct ggcaatcggc cccacgattg gcggctacgg cctgtacatg gtgagcttgg
     7861 gataccttca agccagcgta gctaacctga tcgccacgct ggagccggtt ttcacagcga
     7921 tcttggctta cctgttgctg gcagaacgcc tgaatagcgt tcagattctg ggcggaatct
     7981 tgattttcac aggagtggtg ctgctgcgca tgtatgaagg aagaaggaaa tgacagagtt
     8041 acttttgggt ccagtcatcg gtggccttac acatgattcc gcttacctgt gggggcgggc
     8101 ggatgggccc agcgttttac acgcctggtt gggtcgcaat cccgacctga gcgacgcccg
     8161 tcttgcaggg acttctctcc ccctgtcatc cgatacggga ttcgccgggg tggcggtaac
     8221 taaagagtta caaccgaact ctcgctacta ttacgccctc accctgagcg gctcgccgcc
     8281 cagccccgca acggagggat tttcatcttt tactaccgcc ccatctcccg gagagcggct
     8341 ctctttctcc ttcgcattcg gctcttgctt tcgtccagaa aacgcggatg gcgggcgcat
     8401 ctttgacgcc atcgaggagc aaagaaaggc tgatgatctg cgctttatcc tgctcctggg
     8461 cgaccagatc tatgctgacg cctaccagca caatggcatc ggcaagatcg cctgtacgct
     8521 gcaagaatac cgggatgtgt acgcttacac ctggtcgcgg ccgcccttgc agaggttgct
     8581 ggcaaacctg ccagcttata tgacgctgga cgatcacgag gtggacgacg actggcgctg
     8641 ggtagacagc aaccgccgct gggcaaacat cccgtggtgg gacaaggtct cgcgctggct
     8701 gaaagggcgc ccgctgcaag agcgtcgctt gccggtgaag cgcgcccagg acgcgcttca
     8761 agcttattgg gaacaccagg ggatgcacgc accaagctat aagcttactc cccagctcaa
     8821 ccaagctggc caatacgagc tggatgtcaa agaccccggc tcgctagcct acaccttcac
     8881 cttcggcgcg gcggcgttct ttgtgctgga cacgcgctcg atgcgcgttc gcaactcccg
     8941 tgagaagatc atgctgggcg agggacagtg gcaggcgctg aaaacctggc tgcgggcggt
     9001 taaggatgat tatccaatca agttcctggt cacttcctgt tcgatgcttt accccatgtg
     9061 gcatgacctg cctcgtgacc gatggaccgg ctacccagaa gagcgggacc gcttgctgca
     9121 ctttcttgca gccaacggga tagatggtgt ctacctgcta gcaggagatt tgcactcatc
     9181 tcatgcggtg tatgccgaac tctatggccc cgaggggcgt tctttaccct tgtgggagtt
     9241 ctgttcgagc cccttcgaac aagattgcaa ccgctggggt cgctatacct accggccatt
     9301 gcacaccagc ccggtgaaag tgagctatcg cgccttcagc gtacatcagg taaattatgg
     9361 cgtggtaaag gtcgattttt ctcgcaaaga tcgcccaaac gtcactttcc agattcatga
     9421 ccagcaaggg aaaaagctcg aggctatttc ggtgtaattt ttagagaagg tctccctctc
     9481 tcccccactc caagcgatcg acaagcccct cttctccaac caacctgggc aatagagctt
     9541 ctaactgctc tgggtcaccc gtggtgagat aaatcacttc gccctcttcc cgatccggat
     9601 gatgcaaccc gcgcgcctcc agcaccctcc ccacctgccg cgccactgcc ggggcgggat
     9661 ccaccacgcg cacactggta ccgacgattt cttggatcag cgggatgaca aagggataat
     9721 gcgtgcagcc cagcactacc gtatcgaggc ctcttaatag catagggtgc aaggcatctt
     9781 ccaggatgcg gcgcgtttca gagctatcca gattgcccgc ttcgatctgc gctaccagtc
     9841 ccgggcaggt atgctgcaac aaggtcacac cctgggcaaa gcgctccacg accgaagcat
     9901 acaatgctcc ttgaaaggtc gccggggttg ccagcacgcc caccacacca ctatgagtac
     9961 cctcggcggc tggcttgact gccggctcca tgcccacgaa gggcacctgc gggaaggtct
    10021 gacgtaaata ttgcaatgcc gccgccgaag cggcgttgca agctacaacg accagcttag
    10081 cgccctggct cagcaggtaa cgggtgatcg cagcagagaa tg
//
LOCUS       DBNGSX010000555         2007 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_888379, whole genome shotgun sequence.
ACCESSION   DBNGSX010000555 DBNGSX010000000
VERSION     DBNGSX010000555.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_888379"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="AC16CB_16290"
     CDS             <1..246
                     /locus_tag="AC16CB_16290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344084.1"
                     /translation="GVDANGRIERSRDGGLTWQPASHGLEVPWRRHMVERFTQVGDEL
                     LAVLSNGDLIAAPLDTLDWRYIMPEIQGVNVVTWLTL"
     gene            293..1015
                     /locus_tag="AC16CB_16295"
     CDS             293..1015
                     /locus_tag="AC16CB_16295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3344085.1"
                     /translation="MKRLENKIAIVTGGGSGIGRGAAHALAEEGAKVVICGRRLNRLE
                     ETVEAIWGKGGAALAVQADVSKEKDIKHLVQATMEAYGRIDILINNAGVDGGTAIHQV
                     EAAEWDRVMDINLRGVFLAVRHVLPLMRAQAAGHIINISSESGLEYYTGDGVYGTSKH
                     ALNAFGEYVQRENQDLNIRVNTICPGMVVTEMSENSPGLNHAKCLYPEDIADLIIWLL
                     TRRQNIKIGTPILIQTMLNPWE"
     gene            complement(1044..1634)
                     /locus_tag="AC16CB_16300"
     CDS             complement(1044..1634)
                     /locus_tag="AC16CB_16300"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR3344086.1"
                     /translation="MRLGPWLVIRGLILGNVIVVLLTSALSANASSPNPSSVIDAIPV
                     AEQTVAESTVSTEGCLVSQEFPESVRQWCSLITRYAGEHGLPADLVAAVIWQESGGKP
                     QAISRSGAVGLMQIMPRDGIAATFMCINGPCFSGRPSSDQLRDPEFNVQYGTRMLARL
                     LERNGNLRDALKAYGPMDRGYSYADRVLSLYRQYGQ"
BASE COUNT          481 a          566 c          533 g          427 t
ORIGIN      
        1 ggtgtagacg ccaacggtcg cattgaacgc agtcgcgatg gcgggctaac ctggcaacca
       61 gcctcccacg ggttggaagt tccctggcgg cggcacatgg tggagcgctt tacccaggtc
      121 ggcgatgagc tgctggcggt gctttccaac ggcgacctga tcgctgcgcc actggacacg
      181 ttggattggc gctatatcat gcctgagatt caaggggtca acgtggtcac ctggctgacg
      241 ttataattga ctttgctggg gacagattat ccaacaggag gttgatcaaa aaatgaaacg
      301 cttggaaaat aaaatcgcca ttgtgaccgg cggaggcagc ggcattggcc gcggcgcagc
      361 ccatgcgctg gcagaagaag gcgcaaaggt cgtcatctgc gggcggcgct tgaaccgttt
      421 agaagagaca gtcgaggcga tctggggaaa aggcggggct gccctggcag tgcaggccga
      481 tgtctccaaa gaaaaggata tcaaacacct cgttcaggcg accatggagg catacggtcg
      541 catcgatatc ctgatcaaca acgccggcgt ggacggcggc acagccatcc atcaggttga
      601 agcagcggaa tgggatcgcg tcatggacat caacctgcgg ggtgtcttcc ttgccgtgcg
      661 tcacgtcttg ccacttatgc gcgctcaggc tgccgggcac atcatcaata tcagctcaga
      721 gtcgggattg gaatattaca ccggcgatgg tgtctacggc acttcaaaac acgccttgaa
      781 cgcatttggc gaatatgtgc agcgtgagaa ccaggatctg aacatccgcg tcaacaccat
      841 ctgccccggc atggtggtga ccgagatgag cgaaaactcg cctggtctta atcacgccaa
      901 atgcctgtac ccggaggaca ttgccgacct gatcatctgg ctgctcactc gccggcagaa
      961 tatcaagatc ggcacgccga tcttaatcca gaccatgctc aatccttggg aatagcaggg
     1021 tcaagaggca aaaacaccgc ctcttattga ccgtattgac gatacagcga cagtaccctg
     1081 tcagcatatg aatacccccg gtccatcgga ccgtaggcct tcagcgcatc gcgcaagttg
     1141 ccattgcgct cgagcagcct tgccagcatg cgcgtcccgt actgcacatt gaactccggg
     1201 tcgcgcagtt ggtctgaaga cggtcgtccg gagaaacatg ggccattgat acacataaat
     1261 gtagcggcga ttccatctcg gggcatgatc tgcatcaatc ccaccgcccc gctgcgcgaa
     1321 atggcttgcg gcttaccccc gctctcctgc cagatcaccg cagccaccag atccgctggc
     1381 aaaccatgct caccagcata gcgagtgatg aggctgcacc actggcgcac gctttcggga
     1441 aattcctgac tgaccagaca cccttctgtt gagacagtac tctcggctac agtctgttcg
     1501 gcgaccggta ttgcatctat taccgaagat ggattaggtg aagatgcatt ggcagataac
     1561 gccgatgtga gcaaaactac aatcacattg ccaagaatta aacctcggat aaccagccat
     1621 ggacctaagc gcatagggga ctcctaacta aatattgaag tgacgttaaa atagcacatt
     1681 tgttctagtt tgtcaatccc cgcaacgttg tttaattaac acacacgccc tttaccttct
     1741 taggcctggg agggcggggt acgcatcaca gaaacggttt attgggcggc gtcaccgccg
     1801 cccaataaac cgtttttgtg ttaatcatcc cccctctaag ttttgcttgg gagtcgagtt
     1861 caggttccag ctataactgg atatcttctg gctcttcgat cactgatccg acaattgcag
     1921 cttcctcttc cgcttcaaca gccaatggat cgcagtcacg caacagacca aaatccagcc
     1981 cggacagttc ttcgatcacc cctaccg
//
LOCUS       DBNGSX010000556         2045 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1648882, whole genome shotgun sequence.
ACCESSION   DBNGSX010000556 DBNGSX010000000
VERSION     DBNGSX010000556.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1648882"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="AC16CB_16305"
     CDS             <1..561
                     /locus_tag="AC16CB_16305"
                     /inference="COORDINATES: protein motif:HMM:NF016206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HIR3344087.1"
                     /translation="AQRLIERGGLYDVKIEGVQGNLTDHYDPRTRVLRLSRGVYSGNS
                     VASLAIAAHELGHAMQDHQDYMPLRLRSALVPAVNVGSYLGWIFIFIGLLLRAPQLAW
                     VGVIVFSGGALFALATLPVELNASARAKRLLVESGLIVGEDEARGVNAVLNAAALTYV
                     AALVTAVMQLMYYATLVMGMGGRRRG"
     gene            658..1671
                     /locus_tag="AC16CB_16310"
     CDS             658..1671
                     /locus_tag="AC16CB_16310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR3344088.1"
                     /translation="MRKPVFIAILLLGIFFVITRMAEVEEIVRTSQQGDWRFFLLALT
                     AVLAWLINVAVSYKTIFQATGIDEKFTKLILLAAAANFVNVVAPSAGVGGMAVFVSEA
                     QRRGYSTGRVTMAGVLYILFDYAGFLCVLFLGLIVLIRRHNLTAAEVAATTLLVLIAA
                     AMAAFIYIGMQSSKMLRNVLVWMARRINRLLWPFIHRPYLSEERAHSFAQDAAAGLVE
                     LRRNPQSLITPFGLALSNKTLLISILFFVFLAYKTPFSIGTLVAGFSVAYLFLIISPT
                     PSGIGVVEGVMTLVLRSLYVPWGLAALITLTYRGFTFWLPLIFGLIAFRYISRSAEAK
                     IAI"
BASE COUNT          440 a          531 c          539 g          535 t
ORIGIN      
        1 gcccagcgcc tgatcgagcg cgggggctta tatgatgtaa aaatcgaggg cgtacaaggc
       61 aacttgaccg atcactacga tccccgcacg cgggtgctgc gcctctcgcg gggcgtatat
      121 tccgggaact cagtggcttc cctggcaatc gccgcgcatg agctgggtca cgctatgcaa
      181 gatcaccagg attatatgcc gttgcgcttg cgctcagccc ttgtcccggc agtcaatgtc
      241 ggctcatacc tgggctggat tttcatcttc atcggcctgc tgttgcgcgc accgcaattg
      301 gcctgggtgg gtgtgatcgt cttttcgggt ggggcgctct ttgccctggc aaccctgcct
      361 gtcgagctca atgcctccgc ccgcgccaaa cgtttgctgg ttgaatccgg cttgattgtt
      421 ggagaagatg aagcgcgcgg cgtgaatgca gtactgaacg ctgctgcact tacctatgtc
      481 gctgcgctgg taaccgccgt gatgcagctt atgtactacg ccaccctggt gatgggtatg
      541 ggcggcagga gaagggggta agcccgctgg atagcttggg atcaagaggt gttatagaat
      601 cccaagctaa tccctgaatt taagataatc gagtagcaat ttccatacaa taatttcatg
      661 cgcaagcctg tctttatcgc cattctgctt ttgggaatat ttttcgtgat cacaagaatg
      721 gcagaggtag aggagattgt gcgcacctcc cagcaaggcg attggcgatt ctttctcctg
      781 gcgcttacag ccgtcttggc ctggctgatc aatgtagcag tttcctataa aacgatattc
      841 caagcaacag gcatcgatga aaaattcacc aaattgatcc tgctggcggc cgcagccaat
      901 tttgtcaatg tggtggcgcc ctcagcgggg gtggggggga tggcagtgtt tgtctccgaa
      961 gcacagcggc ggggctattc caccgggcgg gttaccatgg caggcgtatt gtatatattg
     1021 ttcgattacg ccggatttct atgcgtgcta tttctcggat taattgtcct tatccggcgg
     1081 cacaacctca ccgccgctga agtggcagca acgacgctcc tcgtattgat tgccgctgcg
     1141 atggctgcgt tcatttacat cggcatgcag tcttccaaaa tgctacgcaa cgtattggtc
     1201 tggatggcgc gccgtataaa ccgcctcttg tggcctttta tccacagacc gtacctctcc
     1261 gaggagcggg cacatagctt cgctcaagac gctgccgccg gtttggtaga actgcggcgc
     1321 aacccgcaga gcctgatcac gcccttcgga ctagcactga gcaacaaaac cttgcttatc
     1381 tcgatcttat tctttgtttt cctggcttat aagactccat tttctatcgg gacgctggtg
     1441 gccggcttca gcgtggcata tcttttcctc atcatatcgc cgacgccatc gggaattggc
     1501 gtagttgaag gggtaatgac acttgtgctt cgctcgctgt atgtgccctg gggcctggcc
     1561 gccttgatca cattgaccta tcgcggattt acattctggt tgccattgat ttttggcttg
     1621 attgccttcc gctatatctc tcgcagcgcc gaggcaaaga tagcgatata aagcaacatc
     1681 cccgtcatgt tttcttcaca tctcctcttg acggcacggg catgcatatg cacgatgaag
     1741 ctatgattgc tgaccttgaa gagctccgca tagccaggcg cgaggatcac tgaccttccc
     1801 cgccaatttc catttggtcg ctgctatgaa tctctgagcg tgcagttaag tataacggca
     1861 ggttagaggt tatccggaag ttgaataatt agtaatggag ataccaatca cacagtattc
     1921 agaattagat cgatgcaaaa gtcggacata acaaaaaaag acgcatagta tgatgtcaga
     1981 tatgcgtctt ttcgaaattt tcagcaagct agcatttatg aaaccttatt cgaccagccg
     2041 ccata
//
LOCUS       DBNGSX010000557         3871 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_125906, whole genome shotgun sequence.
ACCESSION   DBNGSX010000557 DBNGSX010000000
VERSION     DBNGSX010000557.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_125906"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            353..2860
                     /locus_tag="AC16CB_16315"
     CDS             353..2860
                     /locus_tag="AC16CB_16315"
                     /inference="COORDINATES: protein motif:HMM:NF028237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR3344089.1"
                     /translation="MPYILILLLLLNAGLAILLTTKIAQLPRTPSTRALILLALATSL
                     WSAGYAMEFYSPSLEMMLFWAKVEYLGIVTIPVAWFVFSLSYVSPLSYLEIFRGRYLL
                     LWIIPGLTLVMVVTNSVHGLIWSEISLAQVGPFSTLALNYGLWFWVHLIYSYTLLLIG
                     SILLVNVLLNTIRFQRWQTGLVLIAILIPWISNFLYISDFNPLPYIDLTPFAFFFACM
                     LLFISLYQFSLGSSMPITHKSMLEGMSAGIMVLDSQNRILDLNKATQKIIDLPGTKAV
                     GLPITQVMPWMSEQISLYTGKEFITKVQVGQGTDQHTYQVSITPLSGRDDSIRGNLLV
                     VRELTKQSKVSKNSQQTDWMQEIINLEPDAMLLLNREAKISLANLEAENIFGYLRDEM
                     LDLGLDSLIPERFHHQHRENVGTYFSDPVARPMGAQMSLYARRRDGSEFPVDISLNPL
                     KIGEDLFVACAVRDISERKRAEMALREREETYQALFEHSNDAIFLLSPSRVHLQVNQK
                     AADLLGYTREELIGMSIFDIVVPTEYPEALNRYASLTRGERVPIYERNFRKKDGSTIT
                     VEINLSLIRDNDGKPVMIQSIVRDISERKMIQQKQLEMLEEIKHSRENLRVMAARIEN
                     TREEERRDLADKLHDRVGQNLTGININLNIISNLVIREDFNAIQSRLEDTIELVEETT
                     RLVRNVMAELHPPLLDDYGLFSALRWYIDKYSQRTNIQTILLGEECQPRLPAHVELVL
                     FRIVQEALNNITKHAQADQVTVILNSTSQIATLSVKDNGRGFDAVLLSKSDKEPHWGL
                     LNMQEKAHSIGGRMMITTAPGQGVNISVEVPREVRDD"
     gene            2853..3497
                     /locus_tag="AC16CB_16320"
     CDS             2853..3497
                     /locus_tag="AC16CB_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3344090.1"
                     /translation="MIKVLLADDHTIIREGLKVYLEMQSEIQVVGEATNGSEAVEKAC
                     QLEPDVVVMDISMPELNGIEATKKIIQNCPQTRVIILSMLGTPEHVFKALQAGALGYL
                     LKESASRDVVDAVFSVYAGKRYLSEPITNILVSDYLLIHGSENVQDPLESLSQREREI
                     LLLVVEGKSSAEIGKMLYLSPKTVETYRSRMMKKLGVSDLAGLVKYALQHGLID"
     gene            complement(3654..>3871)
                     /locus_tag="AC16CB_16325"
     CDS             complement(3654..>3871)
                     /locus_tag="AC16CB_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344091.1"
                     /translation="SAYYLTAWLPNGKEMRERGIVADYLRHPSAPLNVILETLLLSAD
                     ERLRSGDYAEVELLLQAVNAALDIFAP"
BASE COUNT         1050 a          894 c          960 g          967 t
ORIGIN      
        1 gccatcgttg ggatctttgg ccacaaggat cagggccagc tcaaacagct tttcctcagc
       61 cgcctatcca agtaaatcca gctcaaagca aatagctgaa ggatggatca tctcccaatc
      121 tcccatcaag gggggttggg agagtgcttt ttaaggctca aataaatctc ttgcggtata
      181 gggaaaacag attattttaa gtgctcccag aaataggttg ctattcaaca cggtcttgca
      241 gataattgct acggatatta tgattaaaca aacaacttct gttaatatac atcgagagaa
      301 atcgatcgtt gtatctagta tctagttctg gaaagaagcg ggtttaacaa aattgcccta
      361 tatcctaatt ctcctcctgc tgttaaacgc tgggcttgcc atactcctga ccacgaagat
      421 cgcccaatta ccacgaacac ctagtacgag ggcgttaatc ttattagcac tggctacttc
      481 gttgtggtca gcgggatatg caatggaatt ttatagcccc agcctggaga tgatgctctt
      541 ctgggcaaag gttgaatatc ttgggatcgt taccataccc gttgcctggt tcgtgttttc
      601 attgtcctac gttagtcccc taagttatct ggaaattttc cgcggcaggt atttattgct
      661 ctggattatc cctgggctca cattggttat ggttgtgaca aattctgttc atggtcttat
      721 ctggagcgag atttctctcg ctcaagttgg tccattctca acactggctt tgaattatgg
      781 tctctggttt tgggtccacc tgatctactc ttatacactt ctcctgatag gctcgatttt
      841 gctcgtgaat gtgctgctaa atactatccg gtttcaacgc tggcaaaccg gtctggttct
      901 gattgccatc ctcattcctt ggatcagcaa tttcttatat atttccgatt tcaatccgct
      961 gccttatatt gatctgacac catttgcgtt cttctttgca tgtatgctgt tatttataag
     1021 cctttatcaa tttagcctgg gatcatccat gccaattacg cataagtcta tgctcgaggg
     1081 gatgtcagca gggataatgg ttctcgattc gcaaaacagg atcttggatt taaataaggc
     1141 tacccaaaaa attatcgatc tgccaggtac caaggcagtc ggattgccta ttacacaggt
     1201 catgccatgg atgagtgaac aaatcagctt gtatactggc aaagaattta tcaccaaagt
     1261 gcaggttggc cagggaaccg accaacatac ttaccaggta tctataacac cgctttcagg
     1321 cagggatgat tcaattagag gcaacctatt ggttgttcga gagctaacca aacaatccaa
     1381 ggtgtccaaa aatagtcaac aaactgattg gatgcaagaa ataataaatt tggaaccaga
     1441 cgctatgttg ctcctaaacc gagaggccaa aatttccctg gcgaacctag aggctgagaa
     1501 catattcggc tacttgcgcg acgagatgct tgatctaggt ctggattctc tgataccgga
     1561 acgttttcac caccagcata gggaaaatgt cggaacctat ttttctgatc cggttgccag
     1621 gccgatgggc gcacagatga gtctttatgc aaggcggaga gacggcagtg aattccctgt
     1681 tgacatcagc ttgaatccac taaaaatagg agaagaccta ttcgtagcct gcgcggtgcg
     1741 ggatatcagc gagcgaaaac gggcggaaat ggcattgcga gagcgcgagg agacctacca
     1801 ggcgttattt gaacattcca atgacgcaat atttttgctt tctccctcaa gggtccacct
     1861 acaagttaat caaaaggccg ccgacttgtt gggctacacc cgggaagagc taatcgggat
     1921 gagcatattc gacattgttg taccaaccga gtatccagaa gctttgaaca ggtatgccag
     1981 tttgactagg ggagagagag tgcctatata tgaacgcaac ttccgtaaaa aagacgggag
     2041 caccataacg gttgaaatca acctctccct aataagagat aatgatggca agccggtcat
     2101 gatacagagc attgtgcgcg acatctcaga gcgaaaaatg atccaacaaa aacagctcga
     2161 aatgttagaa gagataaaac attcacgcga aaatctgaga gtgatggctg cacgcatcga
     2221 aaacacccgc gaggaagagc ggcgcgacct agctgataaa ttacatgacc gtgtcggcca
     2281 aaacttgact gggatcaata taaatttgaa tatcatcagc aacctggtga taagggaaga
     2341 tttcaatgca attcaaagtc gcctggagga caccattgaa ctggtcgagg aaaccacccg
     2401 ccttgtgcgc aatgtaatgg ctgaactgca cccgcccttg ctggacgatt acgggctgtt
     2461 ctcggctctg cgctggtaca tcgataaata ttcccagcgc acaaatattc agacgatatt
     2521 attgggtgag gagtgccaac cccgccttcc ggcacatgtt gagctggttt tattccgcat
     2581 cgttcaggag gcgttaaata acatcaccaa acacgcccag gcagaccagg tcacggttat
     2641 attaaattcg acctctcaaa ttgcgacctt gagcgtcaaa gacaatggca ggggtttcga
     2701 tgcggtgctt ttatcgaaat cggataaaga gccacattgg gggctgctca acatgcagga
     2761 aaaggcgcat tcaattggag gtcgaatgat gatcaccacg gctccaggcc agggggtgaa
     2821 tatctcggtt gaagtaccaa gggaagtgag agatgattaa agtattgctg gcagacgatc
     2881 acaccatcat cagggaggga ttgaaggtct acctggaaat gcaatccgaa atccaggtcg
     2941 tgggtgaggc gaccaacggg agcgaggccg tcgagaaggc ctgtcaattg gaaccagatg
     3001 tcgtggtgat ggatatttcg atgcccgaac taaacggcat cgaggcaacc aaaaaaataa
     3061 tccagaattg tccccagacg cgggtgatta ttctctccat gttggggacg ccagagcacg
     3121 ttttcaaggc gttgcaagca ggcgcgctgg gttatctact caaagaatca gccagccggg
     3181 acgtcgtgga tgcggtcttt tctgtctatg caggcaaacg ctatctttcg gaaccgatca
     3241 ccaatatcct ggtatcggat tatctcctga tccatggttc ggagaacgtg caagatccgc
     3301 tggagagcct gagtcaacgg gaacgtgaaa tccttctgtt ggttgtggaa gggaaatcca
     3361 gcgctgagat cggtaaaatg ttataccttt cgccaaaaac ggtggagacc taccgcagcc
     3421 ggatgatgaa aaaattaggt gtctccgatc tagcggggtt ggtgaaatac gccctccaac
     3481 atggacttat agattaagaa ctgttcacga gtcgtttgcg ctgcttgaag cgaacttggg
     3541 tggtctagtt cccgtttgct caaagggtag aaaaccacaa agcgctcgac aaatatatca
     3601 agcgctttgt gtggggagaa tgggactcga gcacacagcc tactgattcg aactcaggga
     3661 gcaaagatat ccagcgccgc gttcaccgcc tgcagcagca gctccacttc ggcatagtcc
     3721 cctgagcgca ggcgctcatc cgctgagagc aggagtgtct ccaggatcac gttcagcggg
     3781 gcggatgggt gccgcaggta gtctgccaca atcccccgct ctcgcatctc tttcccattg
     3841 ggcagccagg cggtcaggta ataggccgat g
//
LOCUS       DBNGSX010000558         2678 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1580369, whole genome shotgun sequence.
ACCESSION   DBNGSX010000558 DBNGSX010000000
VERSION     DBNGSX010000558.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1580369"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="AC16CB_16330"
     CDS             complement(<1..514)
                     /locus_tag="AC16CB_16330"
                     /inference="COORDINATES: protein motif:HMM:NF024139.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3344092.1"
                     /translation="MQVEWLSLSDVAKLLGIHPSTVRLWSDKGVLPVHRTQGGHRRYL
                     RSELDLWIQSQRVGGTNEVHLVVQNALKNTRFQISEGRLEAEAWYAKLDNEAREQYSK
                     SGRTLLEGLFGYLASDGAMAVAEARSLGYEYASRGRRYGLNVTEAVHAFLFFRNVLMD
                     SMLTVYEAASV"
     gene            complement(554..1855)
                     /locus_tag="AC16CB_16335"
     CDS             complement(554..1855)
                     /locus_tag="AC16CB_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCD family MFS transporter"
                     /protein_id="HIR3344093.1"
                     /translation="MLLKRLQLGLIHVAVAMTLVPINSTLNRVMIKELALSATLVALL
                     ASLPYLFSPIQMAIGSFSDRHPILGLRRSPYIALGLILCVLGVVLSPQAAFLLSSNFW
                     AGFGLGLVTFGAWGMGFNFATVSYLSLASELSGEKGRSKTIAVMWFMMISSIIVTAIA
                     LSQMVDPYTPQALSRAFWIIGLAALVLGSLGLLGLERRSVGGQTASEGYSWGIMLAAI
                     LENRQARLFFWYLTILLAAILGQDILLEPFGGEAFGMSVQATTRITSLWGGCVLLALL
                     LAGFLEGRLGKRVLARWGALSALLGFALIVVSGITLSQGVFYLGVVLLGVGTGLSTVS
                     NLSLMLDMTTAENVGLFMGAWGMSNAVSRLLGTVLGGVVRDGVNQVAGNPLVGYISVF
                     AIEAGLLAVSLLLLRRIDVSAFQKKAASISVVERAAMAGEV"
     gene            complement(2035..>2678)
                     /locus_tag="AC16CB_16340"
     CDS             complement(2035..>2678)
                     /locus_tag="AC16CB_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR3344094.1"
                     /translation="LYDRLRERQARPVRQHFDAALDYQNKSVRFLENHDEPRAAKVFS
                     SDEHRAAAVLTYLSPGLRFFHQGQIQGWQTKIPVQLCRAPDQPTDRLVNASYLRLLEL
                     LHDDILRNGEWRLLECTPAWENNASWDSLIAFSWQGSGDRQWLAVVNYAPHPSQGYVR
                     LPFPELGGATLRLKDLLSSAGYDRQGDDLLTHGLYLDLPPWEVNVFEVVRLTE"
BASE COUNT          678 a          836 c          685 g          479 t
ORIGIN      
        1 gcaccgaggc agcctcatat accgtcaaca tcgaatccat caatacattg cggaagaaca
       61 agaaggcatg caccgcctcg gtaacgttca agccgtaacg ccgtccccgc gaggcgtatt
      121 catagcccag agaacgcgcc tcggcaacag ccatagcccc gtcggaggct aaatagccaa
      181 ataagccttc caggagagtc cggccactct tgctatactg ctcgcgggcc tcgttatcca
      241 gtttggcata ccacgcctca gcctccaggc gcccttcact gatctggaag cgggtgttct
      301 tcaaagcgtt ctgcaccacc aggtgcactt cgtttgtccc accaacgcgc tgcgattgaa
      361 tccacaagtc tagctcactg cgcaggtagc ggcggtggcc gccctgtgtg cgatgaaccg
      421 gcagcacgcc cttatccgac cacaagcgca cagtgctggg atggataccc agtaacttgg
      481 ccacgtcgct gaggctcagc cactcaactt gcatctagcc ctcacctcga tatggatttg
      541 aaaattcatc tgatcacacc tcgccagcca tagccgcccg ctctacaaca gagatcgaag
      601 cggctttttt ctgaaaagcg ctcacgtcga tgcggcgcag caacaacagg gagaccgcca
      661 gcaagcctgc ctcgatggcg aacacgctta tgtagccaac tagcgggttg ccagccacct
      721 gattcacccc atcgcgcacc acaccgccca ggacggtgcc aagcaagcgc gagacagcat
      781 tcgacatgcc ccacgccccc atgaacaagc ccacattctc agcggtggtc atatccagca
      841 tcagcgacag gttagagacc gtggataagc ctgtcccaac gcccagcaac accacgccca
      901 agtaaaacac tccctggctg agggttatac cactcaccac gatcaaggca aagcccagca
      961 gggcactcaa ggcaccccag cgggccagaa cgcgtttgcc caaacggcct tccaggaagc
     1021 cagccaataa caacgccagc aatacacagc caccccaaag ggaggtgata cgcgtcgtag
     1081 cctgtaccga catcccaaag gcttcgccgc caaaaggttc caacaggata tcttgcccga
     1141 ggatagctgc cagcaaaata gtcaaatacc agaagaacaa cctcgcctgg cggttttcca
     1201 ggatggcggc tagcatgatc ccccaggaat aaccttctga ggcggtctgc ccgccaacag
     1261 atcgccgctc cagccccaac aatcccagcg acccgaggac caaggctgcc agcccgatga
     1321 tccaaaaggc gcgcgagagg gcttgcggtg tgtaggggtc taccatctgg ctcaaggcaa
     1381 tcgcagtgac gataatgctg gaaatcatca tgaaccacat cactgcgatg gtcttactgc
     1441 gtcccttctc accggagagc tccgatgcca atgacaggta ggagacggta gcgaagttga
     1501 aacccatacc ccacgccccg aacgtgacaa gcccaaggcc aaagccggcc caaaagttac
     1561 tgctcagcag gaaagccgcc tggggcgaga gcaccacgcc cagcacgcac aggatcagcc
     1621 ccaaggcaat gtacgggctg cggcgcaatc ccaaaatggg atggcggtca gagaacgatc
     1681 cgatagccat ctggataggc gagaagaggt atggcagcga agccagcaac gccaccagtg
     1741 tggccgagag tgccagctct ttgatcatca cccggttgag cgtgctattg atgggcacca
     1801 gggtcattgc cacagcgacg tggatcagcc ccagctggag acgtttcagc agcattatcg
     1861 tcctcaattt attccattta ataattgtga agccagtgcg tccaggctgg aaaaacaaat
     1921 ccagacaacc aataattatg cctcagatta tcaggattta cccctcttcg tcaatgtaga
     1981 tttaggtagg ttttctatag aaaaaagtaa gcaaatctac aggctttgaa cagctcactc
     2041 ggtcaatctg accacctcga acacattcac ctcccaaggg ggcaggtcga gatagagacc
     2101 atgcgttaat aggtcatcgc cctggcggtc gtagcccgca gagctcaaga ggtccttaag
     2161 tcgcagggtt gcgccgccca attcagggaa aggcaatcgc acgtaaccct ggctagggtg
     2221 aggcgcatag ttcaccaccg ccagccattg ccgatcaccc gaaccttgcc atgaaaaagc
     2281 gatcaagcta tcccaggaag cgttattctc ccaggcaggg gtgcactcca gcagccgcca
     2341 ctcgccattc ctcaatatat cgtcgtgtaa cagttccagt aagcgcaaat aactggcgtt
     2401 taccagcctg tctgtgggtt gatctggggc gcggcacagc tggacaggga tcttcgtctg
     2461 ccatccctgg atttgaccct ggtggaaaaa acgcagtccc ggactcaggt aggtcagcac
     2521 ggccgcggca cgatgctcgt cagatgagaa taccttcgcg gcgcgcggct cgtcgtggtt
     2581 ctccaaaaag cgcaccgact tattctggta atccagggca gcatcaaaat gctggcgcac
     2641 cgggcgcgcc tgccgctccc gcaggcggtc atatagcc
//
LOCUS       DBNGSX010000559         2083 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_333389, whole genome shotgun sequence.
ACCESSION   DBNGSX010000559 DBNGSX010000000
VERSION     DBNGSX010000559.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_333389"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="AC16CB_16345"
     CDS             complement(<1..371)
                     /locus_tag="AC16CB_16345"
                     /inference="COORDINATES: protein motif:HMM:NF014750.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HIR3344095.1"
                     /translation="MSIDLSTGKTGEIEIPQEWERDYIGGASLAARLLYDHLTPDLDP
                     LSARAPLLFLNGPLSGTAGPAVGRFVISARSPATGLWGESNCGGFWGPELRKTGYDGL
                     WITGRAESPVYLWIQNNQVEIR"
     gene            510..1115
                     /locus_tag="AC16CB_16350"
     CDS             510..1115
                     /locus_tag="AC16CB_16350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011011896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIR3344096.1"
                     /translation="MGIRIHPTAEVSEKATIGEGSSIWHQAQVRENVKIGMNCIIGKG
                     VYVDAGVSIGDNVKIQNYVSVYHGVTIEDGVFVGPHVCFTNDLRPRAINPDGSLKSAD
                     DWVLGLTLIKRGAALGANSTIRCGTTIGEWAMIGSGSVVTRDIPSNGLAWGNPAQLHG
                     FVCPCGEKLEKTGESGNQIVATCPICGRITEIAKKDWKGII"
     gene            1112..>2083
                     /locus_tag="AC16CB_16355"
     CDS             1112..>2083
                     /locus_tag="AC16CB_16355"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR3344097.1"
                     /translation="MINIAKPMIGSEEKQAVLEVLDSGMLAQGPRVKAFEEAFADMCG
                     VKYAIATSSGTTSLHTALLAHGVGPGDEVITSSFTFIASANSILFVDARPVFVDIDPR
                     TFNLNPALIEAAITPRTKAIMPVHLYGLSCDMNPIMEIASKYNLLVIEDACQSHGATY
                     KGRAVGSFGAGTFSLYPTKNMTSGEGGMITTNDEAIAEHCRVIRQHGMRRRYYHDELG
                     FNFRMTDLQAAVGLEQLKKLGKFNQARAQNARFLSSHLKGVGVPFVPDGHEHVYHQYT
                     IRVPGGRRDALVEHLNKQGVGCMVYYPVPIHKQTFYVNELGYNMSLTE"
BASE COUNT          567 a          511 c          514 g          491 t
ORIGIN      
        1 cgtatctcga cctgattatt ctgtatccag agataaacag gggattcagc gcgccctgtt
       61 atccacaagc catcatagcc ggtttttcga agctccggac cccaaaaccc accacagttc
      121 gattctcccc acaacccagt ggcaggcgag cgggcagata tcacaaaacg ccccacagca
      181 ggcccagcgg tacccgataa tggtccattt aagaaaagca aaggcgctct tgcggaaagg
      241 ggatcgagat ccggtgtcaa atgatcgtat agcaggcggg cggcgagaga tgctccaccg
      301 atataatctc tctcccattc ttgtggaatt tctatttcgc ccgttttacc tgtggataaa
      361 tctatactca aaatcggctg catacatcaa acctgacgat attataagta acatggctat
      421 gaattagcgt tatactacgt gagagtttac caaatattca aacgagtgaa catagaattc
      481 actaattatt gatgcatcag ggagtattca tgggaattcg catccacccc actgcagagg
      541 tatcggaaaa agccacgatt ggcgaaggta gcagcatctg gcatcaggca caggtgcgcg
      601 agaacgtgaa aattggcatg aactgtatca tcgggaaagg tgtatatgtt gatgcaggtg
      661 tgtccattgg ggacaatgtc aagatacaaa actatgtttc tgtttaccat ggcgttacta
      721 ttgaagatgg cgtatttgta ggtccgcatg tttgcttcac caatgatctg cgcccgcgtg
      781 caatcaaccc agatggttcg ttgaagtctg cagatgattg ggtattggga ttgacgctga
      841 tcaaacgggg ggctgccctg ggggcaaatt caaccattcg ttgtggcacc accatcggag
      901 aatgggcaat gatcgggtca ggtagcgttg tcactcgcga tatcccatca aacggtttag
      961 catggggcaa tcccgcacaa ctgcatggtt ttgtctgtcc atgtggagag aaactagaaa
     1021 agaccggcga atccggaaac caaatcgtag ccacttgccc gatatgcggg cgcataaccg
     1081 aaatcgcaaa gaaagattgg aaaggaatca tatgatcaat attgccaaac cgatgattgg
     1141 ctctgaagaa aaacaagccg tactcgaagt cctcgattcg ggtatgcttg cgcagggtcc
     1201 tcgggtaaaa gcttttgagg aggccttcgc agacatgtgt ggcgtgaaat atgctattgc
     1261 cacctcctca ggaactactt ccttgcacac tgccctatta gctcatggag ttggaccggg
     1321 agacgaggta ataacttctt cctttacatt tatcgcctca gcaaacagca tcctgtttgt
     1381 ggatgccaga cccgtttttg tggatatcga cccgcgcacc ttcaacctca acccggcttt
     1441 gatcgaagct gccatcactc cgcgcacaaa agccattatg ccggtgcacc tttatggatt
     1501 gagttgcgac atgaacccaa tcatggagat agcctccaaa tacaacctgc tggtaatcga
     1561 agatgcttgc cagagtcatg gcgctacgta caaaggtcga gcagttggat catttggggc
     1621 aggcaccttc tcgctctacc ccacaaaaaa tatgacctct ggtgaaggtg gcatgatcac
     1681 taccaatgac gaagccattg cggaacattg ccgagttatc cgccagcatg gcatgcgacg
     1741 gcgttattac cacgatgagt tgggcttcaa cttccgaatg accgatcttc aagcggctgt
     1801 tggcctagaa caactaaaaa agctgggtaa attcaaccaa gcacgcgccc agaacgcacg
     1861 ctttctaagc agccacctga agggtgttgg agtaccattt gtcccagacg gtcacgagca
     1921 tgtatatcat caatatacaa tccgagtccc aggtggacgg cgcgatgcct tggttgagca
     1981 cctcaataag cagggcgtcg gatgcatggt atattacccc gtgccgattc acaaacagac
     2041 attctatgtc aacgagctag gttataacat gagcttgacg gag
//
LOCUS       DBNGSX010000560         4339 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1234356, whole genome shotgun sequence.
ACCESSION   DBNGSX010000560 DBNGSX010000000
VERSION     DBNGSX010000560.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1234356"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /locus_tag="AC16CB_16360"
     CDS             complement(<1..171)
                     /locus_tag="AC16CB_16360"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIR3344098.1"
                     /translation="MAETHNARLHAIVEGYVQGVGFRYFVQENAVTLGLRGWVRNLWS
                     GDVELVAEGDRQA"
     gene            complement(155..1360)
                     /locus_tag="AC16CB_16365"
     CDS             complement(155..1360)
                     /locus_tag="AC16CB_16365"
                     /inference="COORDINATES: protein motif:HMM:NF024876.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HIR3344099.1"
                     /translation="MGKKTIFFILAIILAGNLYVYPVRAQAGEILTSVQYTFGEEITF
                     RGEIPAGVSVESATLFFVAEGELQTRTGAATWDNRGQLLYSADLTQSPMRAFSTVVYW
                     FEVTESGGRVLKSERGSFYYEDNRFEWRTRQSKDFRVHWYEGDTAFAQSLLDVAELGL
                     TNIQAVLPLTLSSPVDIYAYATAQEMRATLQNLGKNWVGAHTDPDLGVMVVSLPRGPE
                     QRLEMERQIPHELMHLMLYQWLAVGYGSIPAWLNEGLASIAELYPNPDYLILLNKALD
                     DDSMLPIVSLCDTFPRDASGAFLAYAEATSFTRFLYQQYGTTGLEALTRAYADGMSCE
                     RGVQTALGSTLTQLERQWRREAFREDALLKAFRNLSPWLIILVLALIVPLGLTLAGTR
                     RKSKDGRDT"
     gene            complement(1365..2276)
                     /locus_tag="AC16CB_16370"
     CDS             complement(1365..2276)
                     /locus_tag="AC16CB_16370"
                     /inference="COORDINATES: protein motif:HMM:NF015509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR3344100.1"
                     /translation="MIDLLTLFANNILPIFLAAGSGYLLSKYLKVIPRTISQVGFYIF
                     SPCLVFMLLTNSQLSGSAILQMSAFTVVSMLAVGMLAWLLGRLLRLERRMLVAVIMGA
                     MFGNSGNFGLSMNMFAFGETALAHASVYFVTSAMLTYTVGVVLASLGKSGLKEAVVGL
                     FKVPGMYAVIVAMIFNGYGWKLPLPLDRTVTLLGNAAIPILMVLIGVQLERAKWDGQP
                     VALGLTNIMRLVVAPVVAVGFTLLFGLRGAALQAAISESGTPTAVLMTVLATEYDVEP
                     SFVTTAVFTSTLLSPLTLTPLLAMLGA"
     gene            complement(2273..3283)
                     /locus_tag="AC16CB_16375"
     CDS             complement(2273..3283)
                     /locus_tag="AC16CB_16375"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR3344101.1"
                     /translation="MSEIEGITIQNLLDAMLEVDRPMDPRYLYRLSDLDPADLAALQN
                     TWLNVPSWRRQAIMEDIEEIGLKDYVLSFEAFARYGLTDSEPRVRELAVRTLWEYEAP
                     DLVPIFVRMMESDADVGVRAVAATALGHYIYLGEIDELPASTLHQLEDKLLQVTSGKD
                     SSLVRRRALEALGFSDREEVPVLIKAAYNSGKNDWLSSALFAMGRSVNRAWNKAVISK
                     LDSDVPGVLSEAARAAGELEIKEAVPRLMQLVEDGDSDVRWAAVWALSQIGGEGVRER
                     LEKLYKQTDDDEEADFIESALDNLDFTEDTSLFDLLDIEEDELDDEFYFDLREDEEDD
                     KD"
     gene            complement(3326..>4339)
                     /locus_tag="AC16CB_16380"
     CDS             complement(3326..>4339)
                     /locus_tag="AC16CB_16380"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HIR3344102.1"
                     /translation="NQLLQFIGGLALLIILHELGHYLAARLFNVQVEEFGIGFPPRLA
                     KIFQAGGTIFSINWIPLGGFARIKGQDDPNVPGGLAAASPWVRLAVLFAGPLMNLSMG
                     VVLSILLFYNLGEPVLDQVQVQEIAPGSPAEQAGLQIGDLILRVGDQDVQSVDDLHEK
                     IYARLGEPTSFTILRDDQSFEITMTPRNPPPEDGAIGIIMANPVKPTTLLKAVPVGVA
                     VAYEYTRSVLLLPVRIFQGEVSPQEGRPIGYKGMYDIYQQIEQPLWFFMVISMSLGIF
                     NLFPIPALDGGRILFTIPEIIFRRRIPQKYENAIHLVGFTMLLFLLIYINLQDFLNPV
                     QLP"
BASE COUNT         1142 a         1245 c         1013 g          939 t
ORIGIN      
        1 cgcctggcgg tctccttctg ccactaattc gacatccccg ctccaaagat tgcgcaccca
       61 accgcgcagc cccagggtaa ccgcattttc ctgaacaaaa tagcgaaagc ccaccccctg
      121 cacatagcct tcgacgatgg cgtgaagccg ggcgttatgt gtctctgcca tcttttgatt
      181 tccttctcgt ccccgccagg gtcaaaccca ggggtacaat gagtgcaaga accaaaataa
      241 ttaaccaggg ggataggttt cgaaatgcct ttagcaaggc atcttcccga aaggcctctc
      301 gccgccactg ccgctccaac tgagtgaggg tactgcccag cgccgtctgc acaccccttt
      361 cacaggacat accgtcggca taagctctgg tgagagcttc caaccccgta gtcccatact
      421 gctgatacaa gaaacgggta aatgatgtag cctcggcata agccaggaaa gcgccagatg
      481 catctcttgg aaatgtatca catagggaaa cgataggcag catgctatca tcgtccagcg
      541 ctttgtttag caagatcaag taatctggat tcgggtatag ctcagctatc gatgccagtc
      601 cttcgtttaa ccaagccggg atgctgccat atcctacagc cagccattgg tagagcatca
      661 ggtgcatcaa ttcgtgaggg atctggcgct ccatctccag acgttgttcg ggtccgcgcg
      721 gcagcgagac caccatcaca cccagatccg gatcggtatg tgctcccacc caattcttcc
      781 ccagattctg gagcgtagcg cgcatctcct gggcagttgc ataggcatag atatctactg
      841 gtgagctcaa tgtcaagggc aagacagcct ggatatttgt cagcccaagc tctgccacat
      901 ccagcaggct ctgggcgaaa gcagtatctc cctcatacca atgaacgcgg aagtccttcg
      961 attggcgcgt gcgccactca aagcggttgt cctcatagta gaaagaacct cgctcgctct
     1021 tcaataccct tcccccgctc tcggtgacct caaaccagta aacgaccgta gagaaagctc
     1081 gcataggcga ttgagtcaga tcagcagagt agagcagttg cccacggtta tcccacgtag
     1141 ccgcgcctgt acgagtttgc aactcgcctt cagccacaaa gaacagggta gctgactcga
     1201 cagatacacc agctgggatt tctccacgga aggtgatctc ttccccaaag gtgtactgca
     1261 cactggtaag aatttcgcca gcctgggcgc gaaccgggta cacatacagg ttgcctgcca
     1321 ggataattgc caaaatgaaa aatatcgtct ttttccccat caaattatgc tcctagcatg
     1381 gccaaaagag gcgtcaaggt cagcggactg agcaaggtgc tggtgaacac tgcagtagtg
     1441 acaaaggatg gctcaacatc gtactctgtc gccagaactg tcatgagaac tgcagtcggt
     1501 gtcccagatt cggagatggc agcttgcagc gcagccccgc gcagtccaaa taacagcgta
     1561 aaaccgactg caactactgg tgctaccacc aaacgcatga tattggttaa tcccagggct
     1621 acaggctgcc catcccactt ggctcgttcg agctggacac cgatcaacac cattaaaatc
     1681 gggatagcgg cattacccaa taaagttaca gtccgatcta gcggcaatgg cagcttccac
     1741 ccatatccat tgaaaatcat cgccacaata acagcataca tgcctggtac cttgaagaga
     1801 cccaccacag cttccttcaa gccagatttg cctagagagg ccagcaccac acccactgtg
     1861 taggtcagca tagccgaggt cacaaaataa acgctggcat gcgctaatgc cgtctctcca
     1921 aaggcaaaca tgttcatgga aaggccaaaa ttacccgagt tgccgaacat ggctcccata
     1981 atcacagcta ccagcatacg gcgttccagc ctcagcaacc ggcccagcaa ccaggctagc
     2041 atgcccacag ctaacatact tacaaccgta aatgcagaca tctgcaggat ggcactgcca
     2101 ctgagctgac tgttggtcag cagcatgaat accaggcaag gactgaagat ataaaaacca
     2161 acttgagata tagtacgtgg aattacttta agatactttg ataataaata acctgagccc
     2221 gcagccagaa aaatgggtag gatgttgttg gcgaacaggg taagcaggtc gatcaatctt
     2281 tgtcgtcctc ttcgtcctca cgcaagtcga aataaaactc atcgtccaac tcatcttcct
     2341 cgatatccag caagtcgaaa agtgaggtgt cttcagtgaa atccaggtta tcgagggcag
     2401 attcgataaa gtccgcctct tcgtcgtcat cggtctgctt atatagcttt tcgagtctct
     2461 cccgcacccc ttcgcctcca atttgtgaaa gcgcccatac ggctgcccaa cgcacatcgg
     2521 agtccccatc ttctaccagc tgcatcaaac ggggaacggc ttctttgatc tccaactcgc
     2581 cagcggcgcg cgccgcttca ctcaaaaccc ccggcacatc gctgtccagc ttggaaatca
     2641 ctgctttatt ccaggctcta ttgaccgatc gtcccatagc gaacaaagcg ctggacaacc
     2701 agtcgttttt acccgagtta taggcagctt tgatcaacac tgggacctct tctcggtcgg
     2761 aaaatcccag tgcttctaag gcacggcgcc gtactaaact agaatccttg ccgcttgtta
     2821 cctggagcaa tttatcctca agctgatgca gcgtggatgc cggtaattca tcaatctccc
     2881 ctaaatagat ataatggcct aaggctgttg cggctaccgc gcgcacgcca acgtccgcat
     2941 cggattccat catgcggaca aatatgggca ccaaatcggg tgcttcatat tcccaaagcg
     3001 tgcgcacagc caactcgcgc acgcgcggct cactgtctgt caacccatag cgggcgaatg
     3061 cttcaaagga taagacataa tcttttagac cgatctcttc aatatcttcc ataatcgcct
     3121 ggcgcctcca ggagggtacg ttcagccagg tattctgcaa ggctgccagg tctgccgggt
     3181 ccaaatccga gagccggtac agatagcgcg gatccatggg cctatccact tccaacatgg
     3241 catccaataa attttgaatc gtgattcctt ctatctcact catggcacaa accatccaag
     3301 cacatcgccc ccacaggcaa gactatcagg gcaattgtac agggttaaga aaatcctgca
     3361 ggttaatgta aattagcagg aataaaagca tggtgaagcc aaccaggtgg atggcgtttt
     3421 catatttttg cggtatgcgg cggcggaaga tgatctctgg gatcgtgaac aaaatccgcc
     3481 caccatctaa agcggggatc gggaagaggt tgaatatacc caacgacata ctgatgacca
     3541 tgaaaaacca cagaggttgt tcgatttgtt ggtagatatc gtacatccct ttatatccaa
     3601 tcggacgacc ctcctgcggg ctaacttcac cttggaagat acgcacgggc agcagcagaa
     3661 cgctacgcgt gtattcatag gctacagcaa cacccactgg caccgctttc aacagcgtgg
     3721 tgggcttgac cggatttgcc ataatgatac cgatagcacc gtcctctggg ggcgggttgc
     3781 gcggcgtcat cgtgatctca aaagactggt cgtcccgcag tatcgtgaag ctggtcggct
     3841 cgcccaagcg ggcataaatc ttttcatgca aatcgtctac actctgcaca tcctggtcac
     3901 ccactcttaa tatcagatca ccaatctgca ggccagcctg ctcagcagga gaacccggag
     3961 cgatctcctg tacttgcacc tgatcgagca ccggctcacc caggttgtaa aaaagtagta
     4021 tcgaaagcac cacacccatg gacaggttca tcaatggacc agcaaaaagc accgccagac
     4081 gcacccaggg acttgcagca gccagcccgc cgggcacatt tggatcatcc tggcctttga
     4141 tgcgcgcgaa gccacctaac gggatccagt taatggaaaa gattgtcccc cccgcctgga
     4201 atatcttggc caagcgtggt ggaaaaccga taccgaactc ctctacttgc acgttgaata
     4261 aacgtgccgc aaggtagtga cctagttcgt gcaggataat aagcaaagcc aaaccaccga
     4321 taaactgtaa caattgatt
//
LOCUS       DBNGSX010000561         3829 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_265063, whole genome shotgun sequence.
ACCESSION   DBNGSX010000561 DBNGSX010000000
VERSION     DBNGSX010000561.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_265063"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..427)
                     /locus_tag="AC16CB_16385"
     CDS             complement(191..427)
                     /locus_tag="AC16CB_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344103.1"
                     /translation="MTWESIGRFAVRAVATGTVGQVVNNVVKATLPENAGRIAKASAQ
                     IGGFIISSMVGDAAADYAAEQVDKLLHRRGGDDA"
     gene            complement(509..694)
                     /locus_tag="AC16CB_16390"
     CDS             complement(509..694)
                     /locus_tag="AC16CB_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344104.1"
                     /translation="MLEKGKAFVVEHKRELVKVGAVVVGIAVGAVVASIMRSPLDDFV
                     LPSVDDNDFVDPADTAE"
     gene            complement(772..981)
                     /locus_tag="AC16CB_16395"
     CDS             complement(772..981)
                     /locus_tag="AC16CB_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344105.1"
                     /translation="MKIVDKVKEVARDEEVQQFAKDVLRAAAVGLLAGALVGVAQKAA
                     TSARGAISDRVQEFKDSRIDVSEEI"
     gene            complement(1049..1303)
                     /locus_tag="AC16CB_16400"
     CDS             complement(1049..1303)
                     /locus_tag="AC16CB_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344106.1"
                     /translation="MASKKKLDDCKTLNDFKGYAQRLERRGKCETEEGSNHFKISTTK
                     GSTYLSRGHQSQQLSKGFISSLIKQFIKIGLAIWVISHFV"
     gene            complement(1308..1712)
                     /locus_tag="AC16CB_16405"
     CDS             complement(1308..1712)
                     /locus_tag="AC16CB_16405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344107.1"
                     /translation="MYPYKDLEELVSFNAIFMHKQFIAVMNVLSTFLFRFNKANGLSK
                     DEDKAFFDLAMDGYVISDDYGLMGSDATMSVNVKLMLHNPNEKWQRDLELKLKQSECV
                     YAAPFPMTELITDIMEIGLSNNVPIAITTFSE"
     gene            complement(1714..1863)
                     /locus_tag="AC16CB_16410"
     CDS             complement(1714..1863)
                     /locus_tag="AC16CB_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344108.1"
                     /translation="MDELSGWEQVALAVFLALVYYIFATVFTSAVAIGIVVALFIFFG
                     WIMGD"
     gene            complement(1856..2110)
                     /locus_tag="AC16CB_16415"
     CDS             complement(1856..2110)
                     /locus_tag="AC16CB_16415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344109.1"
                     /translation="MDEKEKRAAINSLAEAVETVARIRRADIYDFSIVNIGDFGPDMK
                     TKGILIHKHTGEIIEGDICDKWSAAVHSVAGKIAKGGQDG"
     gene            complement(2366..2611)
                     /locus_tag="AC16CB_16420"
     CDS             complement(2366..2611)
                     /locus_tag="AC16CB_16420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344110.1"
                     /translation="MTMEKALIEVLKKTSKFAGVNQLEVKPAVPGVAPPEYVYFNQED
                     LIIAMAYMLADIRSMLAQLVYPEDQGGECGNDVLPEL"
     gene            complement(2586..2936)
                     /locus_tag="AC16CB_16425"
     CDS             complement(2586..2936)
                     /locus_tag="AC16CB_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344111.1"
                     /translation="MTEELKERPQVELPEDPPITEEDIEAAMQQLSEADMRSREQIIE
                     LREKLKGSILENLAVLSSHALIVQGKYERIEETMDVLITFMGKLQGRLETLELFVKQF
                     MEKEEANDDGEGAD"
     gene            complement(2950..3720)
                     /locus_tag="AC16CB_16430"
     CDS             complement(2950..3720)
                     /locus_tag="AC16CB_16430"
                     /inference="COORDINATES: protein motif:HMM:NF040385.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6353 family protein"
                     /protein_id="HIR3344112.1"
                     /translation="MNERLLRFARAARRSLHKNSPTFLIGLGIGGFFTAIYLAIQITP
                     RAVEYLDDHAYGQFKDHGKWRDIPPKDRVKLVWPLYIPSASTALLSTVMIILGNKIHL
                     KRTATLGSLYALSEGALQRYQDKAIEMLGERKAKSIGTALDADEAALRVVDEDHVHNV
                     GGGTELCYDSFSGRFFYSDVEKINAAVNRFNKELLIEQEKGLNELYFELGLPGVEIGD
                     RFGWFIDDGLLEITISAQMTKDDRSCIVMKFVRWPKQL"
BASE COUNT          966 a          994 c          779 g         1090 t
ORIGIN      
        1 tctttcaaca ggtaattcgc tcccggggtc ccctgtttca ggacgaatag gtgctggagc
       61 tttaacacgc cgctcttttt tactgtttcc aggtaattct tctgtcataa tatgttctcc
      121 ttatcaagtt tgacaaaaga aaggtagggc atgtagaaat ccccaccttt ccggtgattg
      181 acggtccttt ttaggcgtcg tctccccctc tacgatgcag caatttatct acttgctcgg
      241 cagcgtagtc cgcggcagca tctccaacca tagagctgat gataaagccg ccaatctgcg
      301 cgctagcctt tgcgatgcga ccagcatttt ccgggagggt agccttaaca acgttgttga
      361 ctacctgtcc caccgtgcca gtagcaacgg cgcgcacggc gaatcgacca attgattccc
      421 aggtcatgat taaatctcct tttcaaaagt gtgagcccat gggggtgcca tgagctcact
      481 atgaaatttc ccgcttacgc attgccgcct attcagcggt atcagcgggg tctacgaaat
      541 cgttgtcatc caccgatggc agaacgaaat cgtccagggg cgaacgcata atagacgcga
      601 caacagctcc gacggcgata ccgactacaa cggcgccaac cttaacgagc tcgcgtttgt
      661 gttctaccac gaaagcttta cctttctcca gcatttggtt cctcctttca aggattatat
      721 aaaaggaaaa agagaaggcc tagattacta ggcccgctct tcgtgggttg attagatctc
      781 ttccgatacg tcaatccggg aatccttgaa ttcctggaca cgatctgaaa tcgcaccgcg
      841 ggctgaggtt gcagctttct gtgcaacgcc taccagcgca ccagccaata aaccgactgc
      901 tgccgcccgt aatacgtcct tcgcaaactg ctggacctct tcgtcccgtg caacttcttt
      961 gaccttgtct acaattttca ttagatttct cctttttaga tatagtggtt aagttccgtt
     1021 tctactatag gggatatttt tttcgcgatt acacgaagtg tgaaataacc cagatagcta
     1081 gtccgatctt gataaactgc ttgatcagac tggaaataaa cccctttgac aattgttgac
     1141 tttggtggcc tctggagaga tatgtagatc cctttgtcgt gctgattttg aagtggtttg
     1201 atccctcttc agtttcacat tttccacgac gttccaatcg ttgggcataa cccttgaaat
     1261 cattgagggt tttgcagtcg tcgagtttct tcttcgatgc catgatacta ctccgaaaat
     1321 gttgttatgg ctatgggaac gttgtttgaa agcccaatct ccatgatatc agttattagc
     1381 tccgtcatgg ggaatggtgc tgcgtaaacg cactctgatt gcttcaattt caattccaaa
     1441 tctcgttgcc acttctcatt gggattatga agcatgagtt ttacgttgac cgacatggtc
     1501 gcatctgacc ccataaggcc ataatcatca gatatcacat agccatccat agccaaatca
     1561 aagaacgcct tatcctcatc cttgctcaga ccatttgctt tattgaatct gaaaaggaat
     1621 gtgctaagta cgttcatgac cgcgataaat tgcttgtgca taaatatcgc attaaacgat
     1681 acaagctctt caaggtcttt gtaaggatac atgtcagtct cccatgatcc aaccaaagaa
     1741 aatgaacagt gcaactacga tgccgatggc aactgcagat gtaaataccg tagcgaatat
     1801 atagtacaca agtgccagga ataccgctag tgcgacttgt tcccagccag ataattcatc
     1861 catcttgtcc tcctttggca attttccccg ccacagagtg aactgcagcg gaccacttat
     1921 cacagatatc cccctcaatt atctcaccgg tgtgtttatg aatcaggata cctttggtct
     1981 tcatgtccgg accaaaatca ccgatattca cgattgaaaa atcgtaaatg tcagctcgcc
     2041 taatcctcgc caccgtttct actgcttctg cgagagaatt tatcgccgct cgtttttctt
     2101 tttcgtccac ttaaatatct ccttttcaag tattagattg aagcgtttag tcgccccgtc
     2161 gaaagtagac gtatatagcg ataaacaacc cgatcaaaat gaccagtatc acaaacgcca
     2221 aaaccaacag gcactgcgac atcaaagcaa ttacaggatc catagaaacc tcccggttct
     2281 atactctcca agttgggtgt gacagatctt acaaaatatc cgttcccacc cgttgtttac
     2341 ctttgaatag gtaggtacag atttttcaca gttcgggcaa tacatcgttt ccgcattctc
     2401 ctccctggtc ttcagggtat actagctggg ctagcatgga ccgaatatct gcgagcatgt
     2461 acgccattgc aatgatcaga tcctcttggt tgaaataaac gtattccggt ggagcaacac
     2521 ctggaaccgc gggcttaacc tctaactggt ttacgccagc aaactttgag gtctttttaa
     2581 ggacctcaat cagcgccttc tccatcgtca ttggcttcct ccttttccat aaattgcttg
     2641 acgaacaatt cgagggtttc cagtctccct tgcagtttgc ccatgaaggt aataagaaca
     2701 tccattgttt cttcaatgcg ttcatacttt ccttggacaa tcagggcatg ggaagagagc
     2761 acggccagat tctcgagtat cgatccctta agcttttcgc ggagctctat aatctgctcc
     2821 ctcgatcgca tatcggcctc gctcaattgc tgcatcgcag cctcaatatc ctcctcggta
     2881 attggaggat cttccggtaa ttctacctgg ggtcgttctt ttaattcttc tgtcatgaat
     2941 atctcctttt catagttgtt ttggccaacg cacaaacttc attacgatgc atgagcgatc
     3001 gtctttcgtc atttgcgcgc tgattgtaat ttccagcaag ccgtcatcaa tgaaccaacc
     3061 gaatcgatca ccaatctcaa ccccaggtaa tccgagttcg aaatacagtt cattcagacc
     3121 tttttcttgc tctatcaaca attccttatt aaagcggttt actgccgcat taatcttctc
     3181 tacatcgctg tagaagaatc gaccagaaaa cgagtcgtag cacaattctg ttccaccccc
     3241 aacattatgc acatggtcct catctactac ccttaaagca gcctcatccg catcaagggc
     3301 agtgccgatt gactttgcct tccgttcacc aagcatctcg attgctttgt cctggtaacg
     3361 ttgtagggcg ccctcagata gcgcgtacaa gcttcccagg gttgctgtgc gcttcaagtg
     3421 tatcttatta cctaggatga tcattacggt gcttaaaagc gctgtagacg cagacggaat
     3481 atatagcggc caaacgagct ttacacggtc ttttggtgga atatctcgcc acttaccgtg
     3541 atccttgaac tgtccgtacg catgatcgtc cagatactcc actgctcttg gggtgatctg
     3601 tatcgccaaa tatatagcgg taaagaaccc tccaattccc aaacctatga ggaatgttgg
     3661 ggagtttttg tggaggctcc gacgggccgc cctagcaaat ctgagtagtc gttcattcat
     3721 ttgtattcct ccaccgattg ctctgcatca gccaacccaa aataaatacg atggcgaaaa
     3781 ctattccacc taggcacatc aagcctaaaa atacgacggt caaacgtgc
//
LOCUS       DBNGSX010000562         3228 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1096419, whole genome shotgun sequence.
ACCESSION   DBNGSX010000562 DBNGSX010000000
VERSION     DBNGSX010000562.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1096419"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="AC16CB_16435"
     CDS             complement(<1..189)
                     /locus_tag="AC16CB_16435"
                     /inference="COORDINATES: protein motif:HMM:NF015019.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HIR3344113.1"
                     /translation="MRTQIMREDKGTEKMQEVWGWIRDGADAYLRQQLKSILPLIVVL
                     TIALFFSVYIVPPTPEALE"
     gene            457..1854
                     /locus_tag="AC16CB_16440"
     CDS             457..1854
                     /locus_tag="AC16CB_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344114.1"
                     /translation="MKTTKSEKTTLLDEADQEKETQKALLKAFTGTLHHFFGSWQDLF
                     KGVRDGRNPELIKYPLEGVLSTGVLMYLFRLGARRQINYQLRGNGPSQAKFEAWFEVD
                     EIPHGDTLNYAFQRVAPAEVQEVVCRMLATLIRKKVLYHWRLYGNFLLAIDGTGVLTF
                     QDRHCEYCLTRKLNNGETLYYHPVLEAKLVTANGFAFSLMTEFIENTDPQADKQDCEL
                     KAFYRLSKRVKARFPHLPICLLLDGLFAGGPTFQLCRDYDWRYMITLQDADLSQVHRS
                     YELLRSQTPQTHKQLRLGPQQEITQEFHWARQIAYRDSQDRHHDLNVLVCKESKPTRR
                     GDPNTTTFKWLTNFIPTAHNVDTLANQAGRLRWKIENEGFNIQKNGGFFLEHPYSQND
                     TARKVFYYLLQIAHLLFQLLEKGSLFRQAFPQGVGSLRNIAFRLLEGWRNLRLSPTAF
                     CNLYSGRYQIRFDSS"
     gene            2192..2968
                     /locus_tag="AC16CB_16445"
     CDS             2192..2968
                     /locus_tag="AC16CB_16445"
                     /inference="COORDINATES: protein motif:HMM:NF012784.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIR3344115.1"
                     /translation="MTEAKNTAQVSALEPKTKLTGKVVKTTLAGVLLDIGQELPGVIH
                     ISQLQKDQVNKPEEVVQIGQTVEAWVRRVKKDRVELTLIEPLAMEWNEIKPETVVKGK
                     VVRLETYGAFVDFGAERPGLVHVSEISRGYIKSPNEIVKEGDEVEAIVLDVNRRKKQI
                     RLSMKALEPEIEGLKPAKKEARKVRTKRGDKKKEAFVMEMEEEPKEPEQTAMQIAWQE
                     ALDRANARNKGQKSKRVKAGSEEQEDILNRTLEKRLPTGS"
     gene            complement(3010..>3228)
                     /locus_tag="AC16CB_16450"
     CDS             complement(3010..>3228)
                     /locus_tag="AC16CB_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344116.1"
                     /translation="ISFVPTWPRIRAGWIILYFLVIQIIPALEMLFSNANYGIAYWAH
                     LGGFFSAVFIFLFLRPEAFARYMSDTSV"
BASE COUNT          883 a          828 c          811 g          706 t
ORIGIN      
        1 ctcaagcgct tcaggggtag gcggcacgat atagaccgag aaaaacaacg cgatcgtaag
       61 gacaacaatc aggggcaaaa tggatttcaa ttgttggcgc agatacgcat ctgcgccatc
      121 ccgaatccaa ccccacactt cttgcatctt ttcagtgccc ttatcttcgc gcatgatttg
      181 tgtgcgcaaa agtagggcct acaaaagacc gagaattgcg acgccaaaca cggcccagat
      241 cgcaacttcc tcaaatgtag tgagtccatg catgtagtac atagtggaat attcctcccg
      301 cgtgaaaaaa aatttaggcg tttccgccta atcaacacgt aaacaggatt ttacaaggct
      361 tgacaaattg tgtcaagaaa tcccctgtgc aaacagtggt gaagaaccaa aggtttttcg
      421 cacaggattt ttcgcacaat gaggtatggt tgggggatga agacaacaaa gagcgaaaag
      481 acaacattac tagatgaggc ggatcaagaa aaagagacgc aaaaggctct cctgaaagcc
      541 ttcactggca cgctgcatca tttctttggt agctggcagg atcttttcaa gggggtcagg
      601 gatggtcgca atcctgagct gatcaaatat ccgttggaag gggtgttgag cacaggcgtg
      661 ctgatgtatc tgtttcggtt aggagcacgg cgacagatca actaccaact gcgtggcaat
      721 gggccttcac aggccaaatt cgaagcctgg ttcgaggtgg atgagattcc acatggagat
      781 accctcaact atgcctttca aagagtggca ccagcggagg tgcaggaagt tgtctgtcgc
      841 atgctagcga ccctgatccg caagaaagtt ctgtatcact ggcgactata tggcaatttt
      901 ttactggcga tcgatggcac cggggtgttg acatttcagg accgccactg cgaatattgt
      961 ttgaccagga agctcaacaa cggggagaca ctctactacc atccggtgct ggaagccaaa
     1021 ctggtgaccg ccaatggctt tgctttctcg ctgatgactg agttcatcga gaatacggac
     1081 ccacaagccg ataagcagga ctgtgagttg aaagctttct atcgtttgag caaacgggtg
     1141 aaggcccgtt ttccccattt gccgatctgc ctgttgctgg atggactgtt tgcaggtggg
     1201 ccaaccttcc aactctgccg ggactacgac tggcgctaca tgatcaccct gcaggatgca
     1261 gatctgtccc aggttcaccg cagctatgaa ctcctacgtt cccagacccc gcaaacccat
     1321 aaacagctcc gcttggggcc gcagcaggag atcacacagg agtttcattg ggcccggcag
     1381 atcgcttatc gcgatagcca ggatcgccac catgacctga atgtgctggt gtgcaaagaa
     1441 agcaaaccaa ctcgccgggg agatcccaac acaaccacct tcaagtggct gaccaacttc
     1501 atccccaccg cccacaatgt cgacacgctg gccaaccagg caggacggtt acgttggaag
     1561 atcgagaatg agggtttcaa catccagaaa aatggcggtt tctttctgga acacccttac
     1621 agccaaaacg acaccgcccg caaggtcttc tactacctgc tccagatcgc tcacttgctc
     1681 ttccagttgc tggaaaaagg cagcctgttt cgccaggctt ttccccaggg tgtcggctcg
     1741 ctcagaaata tcgctttccg gctgctagaa gggtggcgca atttgcgtct gtcccccacc
     1801 gctttctgca acctgtacag cggtcgctac cagatccgct tcgactcatc ctgaaacaag
     1861 cggcatgcca gcccacttcg agtgccctgc ctgcgggtta ggcctctgcc aggcaagatc
     1921 tcgttgtgaa tctgacatgc cgtacttctt attttatcct accctgttcc ctccccgttt
     1981 tatttctgtt gacatcctat gaggccatcc ctgcccgttt ggcggagctt gctgcacgtc
     2041 tgttattgct cgtctggcgt ctgactcatc ccatgccttt tcaccaagtt ggttttcttc
     2101 gcggctgaaa attcacattt taagttgacg agggttggtg aagcaggtat aatcagcctt
     2161 cggcattatc ccccctgata ggagtaacat tatgaccgaa gcgaaaaata ccgcacaggt
     2221 cagcgcgtta gaacccaaga ccaaattgac gggcaaggtg gtcaagacca ctctcgctgg
     2281 cgtgctgcta gacattgggc aggaactacc cggtgtcatt catatatcac aattacagaa
     2341 agaccaggtc aacaaacctg aagaagtggt tcaaataggc cagacagtag aagcctgggt
     2401 gcgccgcgtc aagaaggatc gtgttgaact gaccctgatc gaacccctgg cgatggaatg
     2461 gaatgagatc aagcctgaaa cagttgtcaa aggcaaggtt gtccgcctgg aaacctacgg
     2521 cgcttttgtc gattttggcg cagaacgccc cggcctggta cacgttagcg aaatctctcg
     2581 cggctacatc aaaagcccca atgaaatcgt caaggaaggc gatgaagttg aagccattgt
     2641 tctcgatgtc aaccgcagaa agaaacagat ccgcctaagc atgaaagcgt tggaacctga
     2701 gatcgagggg ttgaaaccgg ctaagaaaga agctcgaaaa gtccgtacca aacgcgggga
     2761 caagaagaaa gaagcgtttg tcatggaaat ggaagaagag cccaaagagc cagagcagac
     2821 tgccatgcaa atcgcctggc aagaagctct tgatcgcgca aatgctcgca acaaaggtca
     2881 gaaatccaaa cgcgtaaaag ctggctctga agagcaggaa gatattctaa atagaacgct
     2941 tgaaaagaga ttgccaaccg gcagctaatc gaagtcagaa cagtcaggca aacgcctggc
     3001 tgttttttat taaacagaag tatcactcat gtaacgcgca aatgcttccg ggcgtaaaaa
     3061 cagaaaaata aaaactgccg aaaagaagcc acccaggtgg gcccagtagg ctattccgta
     3121 atttgcattt gagaacaaca tttccaatgc cgggatgatc tggatcacca aaaaatacag
     3181 gatgatccag ccagccctaa tccgtggcca ggtcgggaca aagctaat
//
LOCUS       DBNGSX010000563        13537 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_958383, whole genome shotgun sequence.
ACCESSION   DBNGSX010000563 DBNGSX010000000
VERSION     DBNGSX010000563.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13537)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13537)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13537
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_958383"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /gene="plsX"
                     /locus_tag="AC16CB_16455"
     CDS             <1..920
                     /gene="plsX"
                     /locus_tag="AC16CB_16455"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIR3344117.1"
                     /translation="VLLPRLKALAGDHPPVRVIHAPEVLEMDDKPATSARHKAENSMA
                     IGMDLLKTNQADAFVTAGNTGGAMANALFRLGRIRGVKRPALTALFPVRTGYCVVLDI
                     GANADCKPEYLLQFAIMGSVYAEKVMGVEKPRVALISNGEEAGKGNELIKETYPLIKA
                     SGLNFIGNVEGKELFGGQADVVVMDGFTGNILLKSSEAVAKLITDMLKQELMSNVQTK
                     VGGLLARPAFTRIKKMLDPGEVGAVPLLGIDGLIFIGHGRSDARALVNAIRVARQAVA
                     SNLIAALRDAIQERIASAPSLASEGHKVL"
     gene            917..2191
                     /gene="fabF"
                     /locus_tag="AC16CB_16460"
     CDS             917..2191
                     /gene="fabF"
                     /locus_tag="AC16CB_16460"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR3344118.1"
                     /translation="MTSNHNHSTRVVMTGLGAVTPLGSSIRQLWEGLLSGQSGIRRIT
                     QFDASNLPCQIAGEIPDFEIDQYMDRKEARRIPRSSQIALAAAVQAVEDAGLPENMPN
                     PERASVVFGTAMGGIDRVDEGIQVLRNQGYGRLNPFTIPAGIPNLPAFLVAQRFQCLG
                     RNSTIVTACATGTQAVGEGSEYIRRGVSDIVIVGGTEALIRDFSIGGFSSMRALPTNF
                     NANPESASRPFDAKREGFIFSEGAGAIVLESLEHAQARGAKIYAELLGHSSSSDGYHM
                     ASPDPYALGPARAMRWALEDANLEPEQVDYINAHGTSTPLNDPTETLAIKIVFGDHAH
                     RLSISSTKSMLGHAMGASGTLEAIVCALSIKKGIIPPTINYEYPDPACDLDYVPNQAR
                     KQQVKIAMSNSFGLGGQNACLVMSSFSTNGVK"
     gene            2188..2892
                     /locus_tag="AC16CB_16465"
     CDS             2188..2892
                     /locus_tag="AC16CB_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344119.1"
                     /translation="MKIYTNEKLVARNRRIAQIASLSGLVVLVGGMIISFQRQDLINY
                     SFAALILGFILSQIGIYFTTRWGRSPRPDELINQALKGLDDKYSMYHYATPASHFLVG
                     PAGLWVLMPRHQRGTISLYKDRWRHTGGGFMQTYLKLFGQESLGRPELEMQNEIEKLK
                     KKLQKMMIEGGLPDIQAALIFTSDDIDIQIGDDETPPAPTLYINKLKEFIRKTAKTKP
                     VSLSKIEEIQQVLSSE"
     gene            complement(2930..3160)
                     /locus_tag="AC16CB_16470"
     CDS             complement(2930..3160)
                     /locus_tag="AC16CB_16470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344120.1"
                     /translation="MSHEESAKNVPWFLWPVWALWRLIALIVGFTGRLIAVVLGFVLI
                     VVGIVVSVTVVGMIIGIPMILIGVLLVIRGLF"
     gene            complement(3296..3778)
                     /locus_tag="AC16CB_16475"
     CDS             complement(3296..3778)
                     /locus_tag="AC16CB_16475"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3344121.1"
                     /translation="MDPSRYTLIPRVLIFLMCGERVLLLKGAPTKRIWPNLYNGIGGH
                     VERGEDVLSAARRELLEETGMVVSDLWLCGVVSVDTGQNPGIGIFVMRGECPEGVLAA
                     SGEGQPEWVDILEIQKLPLVEDLFTLLPRVFQARRNDPPFFASYRYDQNDKLVISIGN
                     "
     gene            complement(3807..4763)
                     /locus_tag="AC16CB_16480"
     CDS             complement(3807..4763)
                     /locus_tag="AC16CB_16480"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0030388 - fructose 1,6-bisphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HIR3344122.1"
                     /translation="MIVTTKKLFELAYGKYAIGAYNINNLEQCMGLFRGNLDSQAPFI
                     IQISKGARSYTHKKMLEGLIRAAEDVFPDSIFAVHLDHGDEEACYDCINSGFYSSVMI
                     DASHEDFENNIAITRRVVEAAHAKGVVVEAELGQLGGVEEHVKVDEADAKLTNPDQAE
                     EFVERSGCDSLAVAIGTSHGAYKFSGSQALRLDVLEKIQQRLPGFPLVMHGSSSVPQE
                     EVERINKAGGQLKGAKGVDADQFKYAAKLGVTKVNIDTDGRLVWTRVHREYFNEHPEN
                     FDLRPPGKVFMGEYAKFIAGKNEKLGSAGQLETVRKLVRQKA"
     gene            4938..5474
                     /locus_tag="AC16CB_16485"
     CDS             4938..5474
                     /locus_tag="AC16CB_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344123.1"
                     /translation="MSDLIDQIMGKVSGDMDPFKKILSKIPGFSGYMERQNRRDSDKV
                     LRETVASRFEEQWQRISNIQRDFVNQGEISYLDDLESAAIQLRTFADRVRRATRGYSG
                     LFDAVKINEEELAQLYQYDAAMLEMADEVGRAIDNVEASVGSDGLPAAIRHLTNTARQ
                     CNTVFDRREEVILGGSSS"
     gene            5509..6561
                     /locus_tag="AC16CB_16490"
     CDS             5509..6561
                     /locus_tag="AC16CB_16490"
                     /inference="COORDINATES: protein motif:HMM:NF013324.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR3344124.1"
                     /translation="MARVFDVIEYPNEMREELVHRFPETEAGTFRIGSQVIVRESQAA
                     VFFRDGSALDVFEPGRHTIVTYNIPLLIDKIGKLFNDRTPFPAEVYFVSMREFVDRKW
                     GTPQPIIVRNPNMGLGVALLQGFGNYSFQIKDPQQFVTQVVGAQGIYRTSDIEGRMRA
                     ILLSKLQDLLGETAAKHSVPELIGLTEEIGAGVRAKAQDDFAAVGLTLKTFYVENLKP
                     SSKSAEELRAMGMLDMQTYTQLQAADALRDAAQNPSGGAGLTAGIGAGMGVGNVISQS
                     LSGMTGGGKAGEQAQPSGGPSAPPPVMTPGEAATFLRVSEEDVIAAITAGDLKAKKIG
                     NAYRISKEALEEYLKG"
     gene            complement(6623..7795)
                     /locus_tag="AC16CB_16495"
     CDS             complement(6623..7795)
                     /locus_tag="AC16CB_16495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3344125.1"
                     /translation="MDKLPPLQSVQAIIQTAFPGILEPEAAEMAESGRVRTYPKNWVL
                     CRENALEEIFYIILDGEVRVTKVIDDVEVRELKILHPGAFFGEMALIHNAPRAATVTT
                     LSPTTVLEIHKPAFDQMLRQNSSVSLAMVREVSRRLRENDEMAIEDLRVKAKELAAAY
                     QQLAEQDYARQEFLTTIAHELRTPLTAAFGYLQAMQAGILHGDTFHQALDTTTRNVKE
                     VISLVNDMLFLYELELILLDFQPTDIGQVVSRVVEEQNKKAVKAGVELRLEVASNIPE
                     INADPKSLERVITVLLDNAIKFSPDGGVTTIEVGFDDYKVRVRVSDSGVGIPEEAMPR
                     LFRRFFRLDEVGGHLFSGAGLGLSIARQVVEQHGGEIHVESQLGKGSTFTINLNRE"
     gene            complement(7826..8389)
                     /gene="rsmD"
                     /locus_tag="AC16CB_16500"
     CDS             complement(7826..8389)
                     /gene="rsmD"
                     /locus_tag="AC16CB_16500"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR3344126.1"
                     /translation="MSGLRVIGGVARGRKLRSVPGVSTRPITDRTKEALFNIIAGDIQ
                     GASLLDLFAGTGSVGIEALSRGASFVRFIDIERNAVQTIQVNLEHTGLGSGAEVLRSN
                     AFRFLERPVDRAFDYVYIAPPQYKGIWKRALLVLDTHLGWLSQDAWAIVQIDPKEYQE
                     VQLNNLVLFDQRRYGSTLLVFYERLVG"
     gene            complement(8386..9369)
                     /locus_tag="AC16CB_16505"
     CDS             complement(8386..9369)
                     /locus_tag="AC16CB_16505"
                     /inference="COORDINATES: protein motif:HMM:NF013381.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR3344127.1"
                     /translation="MNHRQRLEAVLSGGKFDRVPVALWRHFPVDDQNPGDLAGATVEF
                     QRNFDFDLVKVTPASSFCLKDWGVTDEWRGISEGTREYTKRAILHPEDWEKLPVLNPK
                     KGHLGDQLACLRLVVNEIGPNTPVIQTIFSPLAQAKNLVGGEQLLVHLRQYPDALLHG
                     LQTIAESTQRFVEAALETGIAGIFYAVQHAQYHRLSPAEYQQFGCAFDLQILEAAQGL
                     WLNMLHLHGESVMFDLFSEYPVAVINWHDRDTDPSLEVGQNRFPGAVCGGLQRERMMV
                     LGTPEQVTDEARQAIHATNGVRFILGTGCVVPITAPRANFLAARMAVEALK"
     gene            complement(9366..9719)
                     /locus_tag="AC16CB_16510"
     CDS             complement(9366..9719)
                     /locus_tag="AC16CB_16510"
                     /inference="COORDINATES: protein motif:HMM:NF012725.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3344128.1"
                     /translation="MSDSNSVIAQSAEDFLQKAWEHHSNKQDDAAEVSFRRAIQMSPG
                     LFDAYFGLGLVLKAQGRKQEAIEAFNKVLELLEVEISSDQAQRHMMRRLTLGHINMIS
                     SGDWNLEKEIWKRIP"
     gene            complement(9722..10936)
                     /locus_tag="AC16CB_16515"
     CDS             complement(9722..10936)
                     /locus_tag="AC16CB_16515"
                     /EC_number="6.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016851 - magnesium chelatase activity
                     [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase ATPase subunit I"
                     /protein_id="HIR3344129.1"
                     /translation="MVDDQQPASNIQSQVNNPAEVDESPPVRSLRELIDKVTGRGIYP
                     AQPSEESGRAEVLPFPFLAMVGQREMKLALTLALINPAVGGVLLIGPRGTGKTTAVRS
                     LVDLLPEVPRSLCYYGCLPEDIEMGGMDAVCPDCARKYAQNETLAQMDRVRLVELPLN
                     ARLEDVVGGLDERASIHQRMRLRRGILAQADRNLLYVDEVNLLEDDVVDALLDAAAQG
                     SYTVRRGPISATYWSRFVLIGSMNPEEGGLRPQIQDRFGLRVIVRPLDESKERLEVYR
                     RVQAHRANPRHVISAYSQETLQAQHEIQLAGESLPKVSIPDEVAAQGLKLIANLQIES
                     LRAEITLFEAARAYAAADGRKQVSPADLREVASLALRLRRSGFMQEYFARQEEEEAEL
                     TGVIDAVFDTGS"
     gene            complement(10939..11433)
                     /locus_tag="AC16CB_16520"
     CDS             complement(10939..11433)
                     /locus_tag="AC16CB_16520"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR3344130.1"
                     /translation="MDAPPGKTVSSTRVQIAQLMQPEHANNWGNVHGGWIIKLMDEAG
                     ALACIRHAQRRVVTIAIDQTVFRQPIRIGDLVIITAEVSYVGRTSMEAEVQVTAEDPV
                     TGECTHTNTGYFVYVALDDEGKPANVPPLIPETDVHIARMKAGRARQLRRLNQRKNNP
                     EDAD"
     gene            complement(11439..11708)
                     /locus_tag="AC16CB_16525"
     CDS             complement(11439..11708)
                     /locus_tag="AC16CB_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344131.1"
                     /translation="MTPERQVYSVHGHYAGYLSDDPRILRKQSSGYLKPRQSPPRSPM
                     PIHPPATIPLAPMMPELPIGVNDVLEELPDMLPAVDFGDLREDMD"
     gene            complement(11845..12306)
                     /locus_tag="AC16CB_16530"
     CDS             complement(11845..12306)
                     /locus_tag="AC16CB_16530"
                     /inference="COORDINATES: protein motif:HMM:NF016060.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIR3344132.1"
                     /translation="MTSILTNSRERTRFLRFAVVGMIGAVIDFGVANLLVYVFFAPLV
                     IAGTISFISAILSNFFWNRYWTYPDSRSKPIAQQLIQFAVVNGLGLTIRIPILRWLEP
                     VLFRLFSRLPFSLPVFSPDVLAKNLTLAVAVLIVMFWNFFVNRYWTYSDVE"
     gene            complement(12303..13262)
                     /locus_tag="AC16CB_16535"
     CDS             complement(12303..13262)
                     /locus_tag="AC16CB_16535"
                     /EC_number="4.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048040 - UDP-glucuronate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HIR3344133.1"
                     /translation="MRILITGAAGFLGSHLCDRLLAEDHEVIGMDNFITGSPDNLAHL
                     AGNSKFLFIRHDVSNFIYVPGKIDAVLHFASPASPNPNSPYGYVNLPIQTMKAGALGT
                     HNTLGVARSNNARFLLASTSEIYGDPLEHPQTESYWGHVDPIGLRSVYDEAKRFAEAL
                     TMAYHRAHGIDTRIVRIFNTYGPRMHLDDGRVVPNFLQQALRKEPLTVYGDGSQTRSF
                     CYVDDLIDGIVRLLYSNEIQPVNIGNPVETTILEFAHTINRLVDNPAGIVYKPMQRGE
                     GDPQRRQPDISRARQTLGWEPKISIGEGINNTIPYFQSKMGLA"
BASE COUNT         3557 a         3627 c         3134 g         3219 t
ORIGIN      
        1 atgtgttgct gccacgcctc aaagcattgg ctggcgatca cccacctgtg agggtcattc
       61 acgccccgga ggtgctggag atggatgata agccagccac ctctgcgcgt cacaaagccg
      121 aaaactcgat ggcgatcgga atggacctgc tcaaaaccaa ccaggctgac gcctttgtca
      181 ccgccgggaa taccggtggc gcaatggcca atgctctctt ccgcctggga cgcatacgcg
      241 gcgtcaagcg cccagcactg accgccttat tcccggtcag gacaggctat tgtgtggttt
      301 tagacatcgg cgccaatgct gattgtaagc cagaatacct gctccaattt gccataatgg
      361 gctccgtata tgctgaaaag gtgatgggcg tcgaaaaacc gcgcgtagca ctcatttcaa
      421 atggcgaaga ggcaggtaag ggcaatgagt taattaagga gacctaccct ctaataaagg
      481 ctagcgggct aaattttatt ggaaacgtag agggcaaaga actcttcgga ggccaagccg
      541 atgtagttgt gatggatgga ttcacaggca atatcttact aaaatctagc gaggccgtcg
      601 cgaaattaat cactgatatg ctcaagcagg aactcatgag taacgtacaa accaaagtag
      661 gagggctttt agcccgccct gcatttactc gtatcaagaa gatgctggat ccaggagagg
      721 ttggagcagt gccattatta ggtattgacg ggctaatttt tatcggtcac ggaaggtcag
      781 acgctcgcgc tttggtaaac gccatccgcg tggcgcgcca ggctgttgcg agcaatctta
      841 tcgcggcttt acgcgatgcc atccaagaac gcatcgccag tgcaccctcc ttagcctcag
      901 aagggcataa agtattatga cctccaatca caaccacagc acccgggtgg ttatgacagg
      961 tttgggtgca gtgacgccac tggggagctc aataaggcaa ctgtgggaag gcttgttatc
     1021 aggtcaatct ggcatccgta gaatcacaca atttgatgcc agcaacttgc cctgccagat
     1081 tgcgggcgaa attccagatt ttgaaatcga ccaatacatg gaccgcaaag aagcccggcg
     1141 catcccccgc tcatctcaaa tcgcgctagc cgcggcggtt caagctgtag aagatgccgg
     1201 cctgcctgaa aatatgccta atccagagcg tgccagtgtg gtctttggaa ctgccatggg
     1261 cggtatagat cgggtagatg agggcatcca ggtattgcgc aaccaggggt acgggcgtct
     1321 caaccccttc acaattcccg ccggtatccc caacctgccc gctttcctgg tagcccaaag
     1381 gttccaatgc ctcggacgca acagcaccat cgttactgct tgcgcaaccg gcactcaagc
     1441 agttggagaa ggctccgaat acatccgccg cggcgtaagc gacatcgtca tcgtcggtgg
     1501 caccgaggca ttgatccgtg atttctccat cggtggattc tcgtccatgc gtgcccttcc
     1561 tactaatttc aatgccaacc ccgaatctgc ctcgcgccct tttgacgcca aaagagaggg
     1621 atttatattt tccgagggtg ccggagcgat agtacttgag agtctggaac atgcacaggc
     1681 acgcggggcg aaaatatacg ctgaattgct tggtcactct tcgtccagcg atggctatca
     1741 catggcttcg ccagatcctt atgctcttgg accggcacga gctatgcgtt gggcactcga
     1801 agacgcaaat ctcgaaccgg agcaggtaga ttacatcaac gctcacggta catccacccc
     1861 ccttaacgat ccaaccgaaa cgctagcaat caagatagtt tttggcgatc atgcccatcg
     1921 cctgtcaatt agctccacca aatccatgct tggtcacgca atgggcgctt caggtaccct
     1981 ggaggctatc gtttgcgctc ttagcatcaa gaagggtatt atcccgccaa ctatcaacta
     2041 tgaataccct gacccggcgt gcgacctgga ttacgtgcca aatcaggcgc gcaaacagca
     2101 agtaaagatt gcaatgtcaa attcctttgg actgggaggc cagaacgcct gcctagtaat
     2161 gagtagtttt tcaactaacg gagtgaaatg aaaatatata cgaatgaaaa gctggttgcg
     2221 cgcaaccggc gcatagcaca aattgctagc ctcagcggcc tggtggtgtt ggtgggaggg
     2281 atgatcatat cattccagag acaagatctg atcaactatt ccttcgcagc actcatcttg
     2341 ggatttattc tctcccaaat tggtatttat ttcaccacac gttgggggcg tagcccacga
     2401 ccagacgaat taatcaacca ggcgctgaag gggctggacg acaagtattc catgtaccac
     2461 tacgccacgc cagcctccca ctttttagtg ggtcctgccg gactttgggt gttgatgccc
     2521 cgtcaccagc gcggcacgat cagcctgtat aaagatcgct ggcgccacac tggaggggga
     2581 ttcatgcaaa cctatctaaa gctatttggg caggagagcc tgggacgccc tgagctggag
     2641 atgcagaatg aaatcgagaa attgaaaaag aagctacaaa aaatgatgat agagggcggt
     2701 cttcctgata tccaggctgc tttgatcttc acaagtgatg atattgacat acaaattggt
     2761 gatgatgaaa ctcccccagc accgactctc tatattaata agctgaaaga attcatccgc
     2821 aaaacagcaa aaactaaacc tgtctcacta tccaaaatag aagaaattca gcaagtcttg
     2881 agctccgaat aacaaaaaag cgggcaagaa acgcccgctt ttttgttatt taaaacaggc
     2941 ctcgaatgac cagcagcacg ccaataagga tcattggaat acctatgatc atacccacaa
     3001 ccgtcacact tacaactatt ccaactacaa tcaacacaaa gcccaataca acggcaatca
     3061 agcgtcccgt aaagccaaca atcaaggcaa ttaggcgcca gagagcccag acaggccaca
     3121 gaaaccaggg cacatttttt gcagactctt cgtgagacat atccttcact cctcaatatt
     3181 agtagattca ttacttatta cgtaaccccc aataaaaggt ttcaatccaa tctgccactt
     3241 ttgcgcttgt aaggatcccc catcgtaaag ctaacagcaa ttcttaaaat gaaatctaat
     3301 tacctatgct aattaccaat ttatcattct gatcatagcg atagcttgca aagaatggcg
     3361 gatcatttcg tctcgcctga aatactctgg gtagtaatgt aaatagatcc tcaacaaggg
     3421 gcagcttttg aatttccaat atatccaccc actccggctg cccttcacca gatgcagcta
     3481 gcaccccttc aggacactcc ccccgcatca caaagatgcc aatacccggg ttttggccgg
     3541 tatccacaga aaccacacca cacaaccaca aatcggagac taccattcca gtctcctcaa
     3601 gcagctctct tcgggcagca ctgagcacat cttcgccgcg ctctacgtgc cctccgatac
     3661 cattgtatag gtttggccag attcgcttgg tcggcgcgcc tttcaataag agtacgcgct
     3721 caccgcacat caggaatata agtacgcgcg ggatcagcgt ataacgacta ggatccacac
     3781 cttgatccac agccggcatg atctgtttag gctttctgtc gtactaattt ccgcacggtc
     3841 tcaagctggc cggcgctgcc aagcttttca ttctttccgg caatgaactt agcatattcg
     3901 cccataaaaa ccttgccagg cgggcgcaaa tcgaagttct ccgggtgttc attgaaatat
     3961 tcgcggtgaa ctcgcgtcca caccaagcgc ccatcggtat cgatgttcac cttggttaca
     4021 cccagcttgg cggcgtactt gaattgatcc gcatccacac ctttggcgcc tttgagctgt
     4081 ccgccggctt tgttaatgcg ctcaacttct tcctgtggca ccgagctact gccatgcatc
     4141 accagcggaa aaccaggcag cctttgctga atcttttcta gcacatctaa acgtaaagct
     4201 tgggagccac taaatttata cgccccatga ctcgttccaa ttgcaactgc cagggaatcg
     4261 catccagatc tctccacaaa ctcctctgcc tgatctgggt tcgtgagctt agcatctgcc
     4321 tcatccacct tcacatgctc ttctacacca cccaactgac ccagctcagc ctctaccacc
     4381 acccccttgg catgggctgc ctcaaccaca cgccgtgtaa ttgcgatgtt attttcgaaa
     4441 tcctcatggc ttgcgtcgat catcaccgag ctatagaatc cactattaat gcaatcgtaa
     4501 catgcctctt cgtcaccatg gtccaggtgt acagcaaaaa ttgaatctgg gaaaacgtct
     4561 tctgcagccc gtattagccc ttccagcatc tttttatggg tgtaactgcg tgcacctttg
     4621 ctgatctgga tgataaatgg cgcctggcta tcaagattgc cacgaaacaa ccccatacac
     4681 tgttctaggt tattgatgtt atatgctccg atggcgtatt taccataagc aagctcaaag
     4741 agcttctttg ttgtgacgat catgatatac tccttatata ttgcgaaagt gacgcagcta
     4801 ttataaacca tccgagcaac ttttcatggc acagtacgta tatttatcga aggatcatcg
     4861 ttgcctcgaa ttaaaactga ggctataatc aatccataca gactgttgcg aaaacacacc
     4921 aacagaagga gaatgaaatg agtgatttga tcgatcagat catgggtaaa gtcagcggtg
     4981 atatggatcc tttcaaaaag atcttgagca aaatacctgg ttttagcggc tatatggaac
     5041 gccaaaatcg ccgagactcg gacaaggtat tgcgcgaaac tgtcgcaagc cgctttgaag
     5101 agcagtggca gcgcatatct aacatccagc gggatttcgt aaaccaaggc gagatctcat
     5161 acttagatga tctggaatct gccgcaatac aattgcgcac ttttgctgat cgcgtccgcc
     5221 gagcaacgcg tggttattcc ggcttattcg atgcggtcaa aatcaacgag gaagagctag
     5281 cccagcttta ccaatacgat gcagccatgc tggaaatggc agatgaggtg ggacgtgcta
     5341 ttgacaatgt cgaagcatct gtgggcagcg atggcttgcc tgcggctatc agacatctga
     5401 cgaatactgc acgccagtgc aacacggtgt tcgaccggcg agaggaagta attttaggcg
     5461 ggtcgtcgag ttaggtgatt attagactaa aaatcaggag attataaaat ggcaagagta
     5521 tttgatgtca ttgaataccc caacgagatg cgcgaggagc tcgtacatcg ctttcccgaa
     5581 acagaggcag gcacttttcg tattggctca caagtaattg tacgcgaatc ccaggcagcg
     5641 gtctttttcc gtgatggatc tgcgctcgac gtctttgaac caggacgtca caccattgtg
     5701 acctacaata tccccctctt aatcgacaaa attggcaagt tatttaacga tcggacacct
     5761 ttcccagcag aagtctattt cgtctccatg cgtgaatttg tcgaccgaaa atggggtaca
     5821 cctcaaccga tcattgtgcg caaccccaac atgggtttag gtgtggccct tctacaaggg
     5881 ttcggcaatt attccttcca gatcaaggat ccgcaacagt ttgtcacgca ggtagtcgga
     5941 gcacagggaa tttaccgcac atcagacatt gaagggcgca tgcgcgcaat cctgctctcc
     6001 aagctgcaag atttgctggg ggagacggct gccaaacaca gcgtacccga actgatcggg
     6061 ttgaccgaag aaattggcgc cggcgtgcgc gccaaagctc aggatgattt tgcagcagtc
     6121 ggtttgacac taaaaacctt ctacgttgag aatctgaaac cgtctagcaa gtctgccgaa
     6181 gagctgcgtg ccatgggtat gttggatatg caaacttata cgcaattgca agccgcggat
     6241 gcactccgcg atgcagccca aaatcccagc ggcggcgcag gactcactgc cggcatcggc
     6301 gctggaatgg gcgttggaaa tgtaatcagc cagagtctct ccggaatgac cggaggcggc
     6361 aaggcaggcg agcaggctca accctctggt gggccatctg cgccaccccc tgtaatgaca
     6421 cccggcgaag ctgccacttt tctgcgagtt tccgaggaag atgtaattgc tgccattact
     6481 gcaggcgact taaaggcaaa aaagattggt aatgcttatc gcatcagcaa agaggcctta
     6541 gaagaatatc tcaagggata aaagtagtta atcagaggct gataagtaga agcgagcggg
     6601 ctatttgtgc ccgctcgctt tgttattccc tattcagatt tatcgtaaaa gtgctccctt
     6661 ttcccagctg actctccaca tggatctccc ctccgtgttg ctcaaccacc tggcgagcaa
     6721 tggaaagccc cagtcccgca cctgagaaaa ggtgacctcc cacctcatcc aatcgaaaaa
     6781 aacgtcggaa taatcgcggc atggcttcct ctgggatacc aacaccagaa tcgctcaccc
     6841 gtacccggac tttataatca tcgaaaccca cttctatggt ggttacccca ccatctgggc
     6901 taaattttat agcgttatca agcaacacag tgatgacacg ctccaggctt ttgggatcgg
     6961 cgttgatttc tggtatatta gaggcaacct ccagcctcaa ttcaacccca gcttttacag
     7021 ctttcttatt ttgttcctcc acaacccgcg agacgacctg gccgatatca gtgggctgaa
     7081 aatccaacaa aatcaactca agctcatata aaaagagcat atcattgacc agagatatca
     7141 cttctttgac attacgcgtc gtcgtatcca atgcctgatg aaaggtatcg ccgtgtaaaa
     7201 ttccagcctg catggcttga agatagccaa aagccgcagt caggggtgtg cgaagctcgt
     7261 gagcaatggt ggttaagaat tcctgacgag cgtagtcttg ttcagccaat tgctgatacg
     7321 cagccgctaa ttctttagcc ttcacacgca ggtcttcgat ggccatttcg tcgttttctc
     7381 tcaaacggcg gcttacctct cgcaccatcg ccaaagacac gctggaattc tggcgtaaca
     7441 tctgatcaaa cgcaggctta tggatttcaa gcacagtggt cgggctcaga gtcgtgaccg
     7501 tggccgcacg aggagcattg tgaatcagag ccatttctcc aaaaaatgcg cctgggtgca
     7561 aaatttttaa ctctcgcacc tcgacgtcgt ctatcacctt tgttacccgc acctcaccat
     7621 caagaatgat atagaaaatt tcttccaacg cattttcccg gcaaagcacc caatttttag
     7681 gataggtacg taccctaccc gattcggcca tttcggcagc ctcgggttca agtatcccag
     7741 ggaatgctgt ctgaatgatc gcctgaactg attgcagcgg aggtagttta tccatctttg
     7801 acctacatat tattccttag gcaattcacc caactagccg ttcataaaat actagcaagg
     7861 tgctgccgta acggcgctgg tcgaaaagaa ccaggttatt cagctgaact tcttgatatt
     7921 ctttgggatc aatttgcaca attgcccatg catcttgcga gagccaaccc agatgggtat
     7981 ctagcacaag cagggcgcgc ttccaaatac ctttatattg tggcggcgcg atatacacat
     8041 aatcaaaggc ccgatctaca ggcctctcga gaaaacgaaa ggcatttgac cttaacacct
     8101 cagccccaga tcccagaccg gtatgctcca aatttacctg aatggtctgc actgcattgc
     8161 gctcgatatc gataaaccgc acgaaggagg cacctcggct caacgcttcg atacctacac
     8221 tgcccgtccc tgcaaaaaga tccagtagag atgctccttg aatatctcct gcaatgatat
     8281 tgaacaacgc ttctttggtc cggtcagtaa ttgggcgagt gctcacacca ggaaccgagc
     8341 gcagctttcg gccacgggca accccgccaa taacccgtaa tccactcatt tgagagcttc
     8401 aactgccatt cgagccgcca aaaaattagc gcgtggtgcc gtgatcggca caacacagcc
     8461 tgtccccagg ataaagcgca ctccattggt tgcatgtatc gcctggcgcg cctcatccgt
     8521 cacttgctca ggcgtaccca gtaccatcat ccgttcgcgt tgcaagccgc cacataccgc
     8581 gcccgggaaa cggttctgac cgacttccaa acttggatct gtatccctgt catgccagtt
     8641 gataacagcg accgggtatt ccgagaacaa atcaaacatc acgctttcac catgcagatg
     8701 aagcatgttc agccaaagtc cttgagccgc ttctaatatc tgcaggtcaa aagcacaacc
     8761 gaattgctgg tattctgctg gagaaagccg gtgatattga gcatgctgaa cggcataaaa
     8821 gatacctgca ataccggttt ccagagctgc ctcaacaaac ctttgtgtgc tttcggcgat
     8881 cgtctgcaat ccgtggagca aagcatcggg gtactgccgc aggtgtacca agagctgttc
     8941 acccccgact agattcttgg cctgtgccag ggggctgaag atggtctgga tcaccggtgt
     9001 gtttggacca atctcgttga cgaccaatcg caagcatgcc agctggtcgc caagatgacc
     9061 ctttttgggg ttcagcacgg ggagcttttc ccaatcttca gggtgcaaaa tagctcgctt
     9121 cgtgtactcg cgtgtccctt cggaaatacc gcgccactca tcagtaaccc cccaatcttt
     9181 gaggcagaaa gatgaggctg gagtaacttt tactaaatca aaatcgaagt tgcgttgaaa
     9241 ttcaaccgtt gcacccgcca aatctccagg attttgatca tctaccggga agtgtctcca
     9301 cagcgcgacg gggactctat caaattttcc gcctgaaagg accgcttcca gcctttggcg
     9361 atggttcatg ggatacgctt ccagatctct ttctccaggt tccaatcacc ggagctgatc
     9421 atattgatat gcccaagggt caggcgacgc atcatatggc gctgtgcctg atcgcttgaa
     9481 atttcaacct caagcaactc taatacttta ttaaaagctt caatcgcttc ttgtttacga
     9541 ccttgagctt ttagtacaag tcccaaacca aaatacgcat cgaaaagacc gggactcatt
     9601 tggatagccc gccgaaaact cacttcggca gcatcatctt gcttattact atgatgctcc
     9661 cacgcttttt gaaggaaatc ttcagccgat tgtgctatta cggagttgga atcgctcatt
     9721 tttaagatcc tgtatcgaaa actgcatcaa ttacaccggt tagctctgcc tcctcttctt
     9781 cctgacgggc aaagtattcc tgcataaatc ctgaacgcct taaacgcaat gccaatgacg
     9841 caacctcgcg cagatcggcg ggagagactt gcttgcgccc atcagcagca gcataggctc
     9901 gagcggcctc aaataaggtg atctcagcac ggagagattc aatctgcaaa ttggcaataa
     9961 gttttaaccc ttgcgcagcc acttcatctg gtattgaaac ctttggtaga ctctcccccg
    10021 cgagctgaat ctcatgctgc gcttgtagtg tctcttggga ataagcagag ataacatgcc
    10081 gagggttagc ccggtgagct tgtaccctgc ggtaaacctc caaacgttcc ttggattcgt
    10141 ccagaggccg cacaatgact cgcaaaccga agcggtcttg aatttggggg cgaagcccac
    10201 cttcctctgg attcatcgac ccgattaata caaaacgcga ccaataggtg gctgagatag
    10261 gtccacggcg cactgtataa ctgccttgtg cagccgcgtc cagcaaagca tccaccacat
    10321 catcttccaa caaatttact tcatcgacat agagcaaatt acggtcagct tgtgctaaga
    10381 tgccgcgtct caagcgcata cgctgatgga tactagcccg ctcgtccaga cctcccacaa
    10441 catcttcgag acgggcattg agtggcaatt ccaccaggcg cacgcgatcc atttgggcca
    10501 aagtctcatt ttgggcatac ttgcgagcac aatccgggca aaccgcatcc ataccaccca
    10561 tctcgatgtc ttcaggaaga cacccatagt agcataagct acgcggaacc tctggcagca
    10621 gatccaccaa gctgcgaaca gcggtggtct tccctgtgcc acgcggtccg atcagcaaca
    10681 ccccacctac cgctggattg atcaatgcca gcgttaaggc aagtttcatc tctcgctggc
    10741 ccaccatcgc caaaaatgga aatggcaata cttccgcgcg cccagattcc tctgaaggct
    10801 gagcaggata aatcccacga ccagtcacct tatcgatcag ctcgcgcaat gatctcacag
    10861 ggggagactc atcaacttca gccgggttat tgacttggga ctgtatattc gaagcaggtt
    10921 gttgatcgtc aaccattttc agtctgcatc ttctggatta ttttttcttt gattcaagcg
    10981 gcgtaattgc cgggcacgcc cagctttcat gcgtgcaata tgtacatctg tttcggggat
    11041 caatggaggc acattagctg gtttgccttc atcatcgaga gcaacataaa caaagtagcc
    11101 agtattggta tgtgtgcact caccagtcac agggtcctcc gcagtgacct ggacttctgc
    11161 ctccatcgac gtgcgaccca catagctcac ttcagcagtt attatcacca aatctccaat
    11221 gcgaatgggc tgcctgaata cagtttgatc aatagctatc gtaaccaccc gccgctgggc
    11281 atgacgtata cacgcaaggg caccagcttc atccatcagt ttgatgatcc aaccgccatg
    11341 gacattaccc caattgttag catgctcggg ctgcatcaac tgggctatct gaacgcgggt
    11401 ggagctgacg gttttcccag gaggagcatc catcttgctc aatccatatc ctcgcgcaaa
    11461 tcaccgaaat ctactgcggg aagcatatca ggcaactctt ctaagacgtc attgacacca
    11521 attggtaact ccggcatcat tggggccaga ggtatcgtag caggtggatg aatgggcatt
    11581 ggggatcgcg gtggcgactg gcgcggcttt agataaccgg atgactgctt gcgaaggatg
    11641 cgtgggtcat cgctaagata acccgcgtaa tgtccatgga cagagtagac ctgcctttcc
    11701 ggcgtcaccc atccaatcca ttctccttga cgattatata tatacggata cttgagatat
    11761 ccccccacat ctccacttgt tgtatagata gcgatcaagt gcaacttgtt agtcatatct
    11821 attaggaatt atagcatccc aagatcattc cacatcacta tatgtccaat atcgattgac
    11881 gaagaaattc caaaacatca caatgagcac tgcaaccgcc aaggtgagat tttttgccag
    11941 gacgtctgga ctaaacactg gcaacgaaaa aggcagcctg gaaaaaaggc ggaataaaac
    12001 tggctccaac catcgcagaa tgggtatgcg gatcgtcagc cccaagccat tgaccaccgc
    12061 aaattgaatt aattgctggg cgatgggctt agagcgcgag tccggatatg tccagtagcg
    12121 attccagaag aaattgctta atatagctga gataaatgag attgtcccgg ctattaccag
    12181 cggggcgaag aaaacataaa ccagcagatt cgcaacacca aaatcaatca ctgcaccgat
    12241 cattccgaca accgcgaagc gcaagaaacg cgttctttca cgcgagtttg tcagaatgct
    12301 ggtcatgcta gccccatctt ggattgaaaa tatggtattg tattatttat cccctcacca
    12361 atgctaattt ttggttccca gcccagcgtc tggcgtgctc ggctgatatc aggttggcgc
    12421 cgttgcggat cgccttctcc acgctgcata ggcttataaa caattcctgc agggttatcc
    12481 accaagcgat taatggtgtg ggcaaattcc agaatggttg tctccaccgg attaccaatg
    12541 ttaaccggct ggatctcatt cgaatatagc agacgaacga tcccatcaat taaatcatcg
    12601 acataacaaa agctgcgcgt ctgcgatcca tccccataaa ctgtcaaagg ctctttgcgt
    12661 agggcttgct gcaaaaagtt cggcaccaca cgaccatcat ccaggtgcat acgcggaccg
    12721 taggtattga aaatgcgcac gatccgcgta tcaataccgt gcgcacgatg atatgccatt
    12781 gtcaatgcct cagcgaaacg tttggcttcg tcataaaccg agcgcagacc aatcggatca
    12841 acatgtcccc aataggactc ggtttgcggg tgttctaaag gatcgccata gatctcgctg
    12901 gtagaagcca acaaaaatcg cgcattgttc gaacgcgcca cacccaatgt gttatgcgtt
    12961 cccagcgcac ctgctttcat cgtttggata ggtagattta catatccata gggagagttc
    13021 gggttagggc ttgcaggcga agcaaaatgc agcaccgcgt cgattttgcc cggcacgtag
    13081 atgaaattag aaacatcatg gcgaatgaat aagaatttcg aattccccgc aagatgtgcc
    13141 agattatcag ggctgccggt gatgaaattg tccatcccaa tcacttcatg gtcttctgcc
    13201 agcaggcgat cgcacaaatg cgaacccaag aaacccgcgg cacccgtaat taatatacgc
    13261 ataaggtatt atctccaggt aattcgtcta atcaggctgt cgcctgtgat ttgctgagca
    13321 tccccagtta tcgagttgac cagccaaaaa tttccttgat atagaattgc aagggcataa
    13381 tttccatcgc ccggaagatg cgcaggtgac catactacct gctgtggttc cagcccaggc
    13441 tcacccttag gcggaaatag gatattctgg ttcgaaccag aatatcctat ttccgcctaa
    13501 gggtgagcct gggctggaac cacagcaggt agtatgg
//
LOCUS       DBNGSX010000564         2342 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_681769, whole genome shotgun sequence.
ACCESSION   DBNGSX010000564 DBNGSX010000000
VERSION     DBNGSX010000564.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_681769"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="AC16CB_16540"
     CDS             complement(<1..612)
                     /locus_tag="AC16CB_16540"
                     /inference="COORDINATES: protein motif:HMM:NF025670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HIR3344134.1"
                     /translation="MHTIGASNKKITRGLLFATLLLLALTLLVPSGLAAGPSPIAGQT
                     LMGGLSATGSSIPTLGIVQQWSLTAPEPRILDEFGFAVAIDGNTAVVGARNADPNLGG
                     GPIDDAGAAYVYIHNGKTWVLQDELTAKDASPGDTFGVSVAISGNTIVVGATGVNLEE
                     QDEEDDELKDVGAAYVFTRSGGDWVQQVKLTASDREEDDSFGSA"
     gene            complement(992..1083)
                     /locus_tag="AC16CB_16545"
     tRNA            complement(992..1083)
                     /locus_tag="AC16CB_16545"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1047..1049),aa:Ser,seq:gct)
     gene            complement(1155..1241)
                     /locus_tag="AC16CB_16550"
     tRNA            complement(1155..1241)
                     /locus_tag="AC16CB_16550"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1205..1207),aa:Ser,seq:tga)
     gene            complement(1333..>2342)
                     /locus_tag="AC16CB_16555"
     CDS             complement(1333..>2342)
                     /locus_tag="AC16CB_16555"
                     /inference="COORDINATES: protein motif:HMM:NF027484.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HIR3344135.1"
                     /translation="ANQPPGTQFVAATFTPPGTFPSATPPPTQPLQTTASPVPTTTSV
                     PCDRAKFIDDVNFPDNQKVNAGESFVKTWRLGNDGSCTWSTAYAVVFSGGDQMGAPVA
                     VPLTGNVSPGGTIDISVTLKAPDDGGTFRGEFKLRNASNVIFGLGTQNKPFWVQIRVP
                     VATGLLFDFLTQADAAAWTSGLGNDAGSALTFMGASDDANGAAKIVDQVKLETGATSG
                     KILLMFPKHGDNGYVTGLFPAYKVQSGDHLKARLGFMIPSGDSCGSGKVKFQIYSKEG
                     DGELKLLKEWSKSCNNSLLPIDLDLSALKGKEVRFAFTVRAEGAFTDDWAIWNSPRIE
                     H"
BASE COUNT          502 a          642 c          656 g          542 t
ORIGIN      
        1 agccgagcca aaagagtcat cctcttcacg gtccgaggcg gttagcttga cctgctgcac
       61 ccaatcaccg ccgctgcggg tgaagacata ggcagctcca acgtccttca gctcatcatc
      121 ctcttcatcc tgctcttcca gatttacacc ggtagccccc acaacgatgg tattaccact
      181 gatcgccacc gatacgccga aggtatcccc aggggaggcg tctttggctg tgagttcatc
      241 ctgcagcacc caagtcttac cattgtggat gtagacatat gctgccccgg catcgtcgat
      301 gggtcctcct cccaggttgg gatctgcgtt gcgcgcgcca accacggctg tgttgccatc
      361 aatcgccacc gcaaaaccga actcgtccag gattctaggt tctggcgcgg tcagcgacca
      421 ttgctggacg atccctagcg tggggatgct actcccagtt gctgacagac cacccatgag
      481 cgtttggcca gctattggtg atgggccggc tgccagaccg gaggggacca acagcgtgag
      541 agccagcaac aagagggtcg cgaataagag gcctcgtgtt atctttttat tcgatgcgcc
      601 gatggtatgc atattctcct ccacccgaaa gaattaaatg atgactttta ctctcgcata
      661 taattattat ctcactgatt ttagtccaat acgtgttgag cgtcaaacgt agtatgccaa
      721 aatgggggtt atgtgggacc aataaggctg gatgacacgc gagcttgcgg tttgttcttg
      781 aattaataga tgtatataag taagcggggc agatgccgag attcaccctg tccagcgcga
      841 acatccatac acgacagagt tggtcgccct tattcgcggc gatttgcctg cagatagcac
      901 ggctcaccag ttaagagact ggttgctgtc ttcagacggg cagatgttga tcgaggagag
      961 tggatacgta ccgcgatatt aaagggggag gcggagaggg cgggattcga acccgcggta
     1021 gaggtttata gcccctacaa ccgcttagca ggcggcccca atcgaccact ctggcacctc
     1081 tccgtgcgcg ccgagtattt acctcggcgg cttgaattct accatatttc caattgtggt
     1141 tgaggtgatg caggcggagg gagagggatt cgaacccccg gtgggcgtga acccacagct
     1201 gttttcaaga cagccgcctt agaccgctcg gccatccctc ctaagcgatg agattctacc
     1261 atagaggtcc tccccccaca aaataggtca gtggcatctt tggggttgct atggagattc
     1321 ccaaagactc gctcagtgtt ctattctcgg agagttccag atagcccagt catcggtgaa
     1381 ggcaccctca gcgcggacgg tgaaggcgaa gcgtacctct ttgcctttca gtgcagatag
     1441 atccaggtcg atgggcaata agctgttatt gcagctcttg gaccattcct tgagcagctt
     1501 cagctcgcca tctccttctt tggagtagat ctggaatttt acctttcctg agccgcagct
     1561 atcgcccgag gggatcatga agcccaatct ggctttgaga tggtcgccgc tctgcacctt
     1621 ataggcggga aacagcccgg tgacgtagcc gttatccccg tgcttgggga acatcagcag
     1681 gatcttgccg gaggtagccc cggtctcaag cttaacctgg tcgacgatct tggcggcacc
     1741 attggcgtcg tcggaggcgc ccatgaaagt caatgcgctc cccgcatcat ttcctaagcc
     1801 tgaggtccat gcggcggcgt cggcctgggt caggaagtca aaaagcaagc cagttgcgac
     1861 cggtaccctt atctgcaccc agaagggctt gttttgagtg cccaatccaa agatgacatt
     1921 agaagcgttg cgcagcttga attcaccccg gaaggtgcct ccatcatccg gagctttcag
     1981 ggtcaccgag atgtcgatgg tgccacccgg gctcacattt ccggtcagcg gtacggctac
     2041 tggtgcgccc atctggtctc ccccggagaa aactacagca tatgctgtgc tccaagtgca
     2101 tgagccgtca tttcccaagc gccaggtctt cacaaaactt tccccggcat tgaccttttg
     2161 gttatctgga aagtttacat cgtctataaa ctttgcccgg tcgcatggca ccgaggttgt
     2221 cgttgggacg ggagacgcgg tcgtctgaag tggttgtgtg gggggtggcg tggcgcttgg
     2281 aaaggtaccc ggcggcgtga aggtagcggc aacgaactga gtgccgggcg gctggttagc
     2341 ca
//
LOCUS       DBNGSX010000565         8554 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_542831, whole genome shotgun sequence.
ACCESSION   DBNGSX010000565 DBNGSX010000000
VERSION     DBNGSX010000565.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8554)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8554)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8554
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_542831"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="AC16CB_16560"
     CDS             complement(<1..889)
                     /locus_tag="AC16CB_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344136.1"
                     /translation="MTLSRRPVIFQFYVLLTTLEGIVAFYYSFQSQSMERNAWLFGFS
                     PSRLVLGAAMLGLILCMGWLSLKAFTDKRWLASAVSNLALLVSDSRRLILSALSLTMI
                     VIGGLALLSVSSSDRLPDLPYVLQIALERLYPAQIWATLAAVQTLGILYLSIPEAMVR
                     RLYVQPKIIVASLFVSLLAVTIFQLVFLSMQIAIFEEIPDWFWNFRQRGMSFRQLIFL
                     VILILAMFVSRQVLRAPQRGRWNVVLILILGYASQLSFGIAQGQGFDSLRLKYVSSGL
                     SVFSKNACSDVGVLESVRRY"
     gene            1152..2468
                     /locus_tag="AC16CB_16565"
     CDS             1152..2468
                     /locus_tag="AC16CB_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011319520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR3344137.1"
                     /translation="MTTIIFEILIIFLLIQLNGVLAMSEIAVISARKVRLQHRAAEGD
                     AGAQAALEIANQPSHFLSTVQIGITLVGILTGAFSGATIAEELEAYLGHTQIPLLSAY
                     SEVISVSVVVIVITFLSLIVGELAPKQIGLNNPERIASIVAPIMRTISRLTSPLVHLL
                     SFSTSLVLRLIGMRRTTDPEVTEDEIKLMIEQATAGGIFYPEEEEMVEQVFRLADRPI
                     VAQMTPRTEIVWLDLENPIEAIRETLTAARYSYYPVGRGGVDNLLGVVHAKDLLAQCV
                     SGKSLNIEEKIIQPQYLPEGMTILETLKRFRESRTQIAFIIDEFGGIQGLTTITNILE
                     AIAGDFPDAIDAAEQEIVVRKDGSYLLNGMLDIDEFKDLFRIAALPGEEGNSYQTLGG
                     FVMMFLGRIPSAGDYFEWENLRIEIVDMDGLRVDKVLVTPSTVSLQ"
     gene            complement(2499..3554)
                     /locus_tag="AC16CB_16570"
     CDS             complement(2499..3554)
                     /locus_tag="AC16CB_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019499943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIR3344138.1"
                     /translation="MNTLPSTTRIKTLPLPPHFDPGKVAQVWRVPYQALAESASQWAR
                     QHNLQPAAEDHFRVALIAVDMQNTFCLPDFELFVAGRSGMGAVEDNRRLCEFIYRNLG
                     VLTRIIPTMDTHQAMQIFHPIFLVNEQGEHPAPLSLISYQDVEQGRWRFNAAIAPILG
                     IDAAYGQEHLLHYTKVLKEQGKYDLTIWPYHAMLGGIGHALVSSFEEAIFFHSIARQA
                     QPAIETKGQLAISESYSAIGPEVLEDRRGEQIGQKNPRFLGLLDEFDAVVIAGEAKSH
                     CVAWTVDDLLSQIQAYNPLLAHKVYLLEDCTSPVVVPDIVDYTGSADEAFQRFAEAGM
                     HIVRSTDPIASWPGMAG"
     gene            complement(3760..4581)
                     /locus_tag="AC16CB_16575"
     CDS             complement(3760..4581)
                     /locus_tag="AC16CB_16575"
                     /inference="COORDINATES: protein motif:HMM:NF015213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HIR3344139.1"
                     /translation="MLPSFLLSLREGLEAALIIGIVLGALRKIHRQDLSPVVWGGTLS
                     ASAISVIVALALYAVGASLEGSAEGVFEGITVFIAAGVLTWMIFWMHRQSRTIKGELE
                     LGVRHAVSKTGVRALFLLAFLAVLREGVELALFLTASTFAINAQHTILGAVLGMGTAI
                     LLGWSLFTSTVRLDLRRFFQITGALLILFAAGLVAYGVHEFNELGWIPSLIEHVWDIN
                     FILDEKSTLGLMLKALFGYNGNPSLSEVLAYIGYFAAILVGLRRSQEPVASVQNA"
     gene            complement(4611..5060)
                     /locus_tag="AC16CB_16580"
     CDS             complement(4611..5060)
                     /locus_tag="AC16CB_16580"
                     /inference="COORDINATES: protein motif:HMM:NF013629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR3344140.1"
                     /translation="MPDNQDTFIDTLHDHGLRLTPQRELVLQILREASEHLDAEGIWQ
                     NARQYDQDINLATIYRNLNVLSQIGLIQQSFLGEGQKRGYYELIDKPFHYHFACLRCG
                     KVLELLSPPMAQAQQEMERHYGVRIIDAHYKFEGLCPDCMASHANDE"
     gene            5477..7546
                     /locus_tag="AC16CB_16585"
     CDS             5477..7546
                     /locus_tag="AC16CB_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3344141.1"
                     /translation="MNKYADLEIGLHRRELDTYSVDFRFSQPDSETDVRPGRGDAQID
                     LKALQKFLPGSADYSQALTSSLFTDPAVLTAFSQAVTSVQTSGSALRVRLLIGSSAPE
                     LHGIHWETLRNPQTDAPLCTDENILFSRYLSSLDWRPVHLRPKGDLKALVVVANPSNL
                     SDYQNLAPVDVEGETTRARENLGPIHVTTLPPSPSDTAPGSRASLPNLIARLRDENPD
                     ILYLVAHGALTRDEPKIWLEDEDGKAAVTSGTELVTRLRELSERPLLVVLASCQSAAH
                     PTYGEALTALGPRMAEAGIPAVIAMQANVSMKTIAEFMPVFFAELQKDGQIDRALSVA
                     RGTVRQRPDYWMPALFMRLKSGRIWYTPGFGEERGAFEKWPSIIRTISRGLSTPILGP
                     GLYETVLGSPREIARRWAEVYHYPMEPHERESLPQVAQFLAVNQYDLAPFDELEEYLK
                     KDIRSRFGDNLPDELRANGASLDQLIEAAGAIRRQQSEVEPHKVLASLPLPVYITTNL
                     NNLMGSALKEAGKEPQIVLCPWNDYVEELQADFDPGYKPTVERPLVYHLFGRLSEPES
                     VVLTEDHYFDYLIGVTRNKEIIPSVVRSALVNSALLFLGFQLDDWQFRVLFRSVRAQQ
                     GGELLGRYPHIAVQLEPEEGRLLEPERARKYLESYFSAGSNISLFWGRAEDFVKELHS
                     RWKARSA"
     gene            7556..>8554
                     /locus_tag="AC16CB_16590"
     CDS             7556..>8554
                     /locus_tag="AC16CB_16590"
                     /inference="COORDINATES: protein motif:HMM:NF044287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3344142.1"
                     /translation="MTITNTRPNPYVGPRAFRTGEALYGRGRELHELLNLLIAERIVL
                     LHSPSGAGKSSLVYAGLIPRLEEEGFQVLPVARVNQDLPASLGSDERFNRYAFSTVVS
                     LDEALPEKKRLPLEELATISLPDYLERFKAQNLTTVGDTDDLPPMEAEVLIFDQFEEV
                     LTVAPTDLEGKAAFFAQLGAALRDRKRWALFVMREDYIAALDTYLRLVPTRFRNTFRL
                     DLLGVEAAMQAIQLPPKEAGVKFNNNAAQKLVDDLRQVQVQRPDGTMEIQPGPHVEPV
                     QLQVVCFRLWQNLELDDKQISIEDVVSVGDVNQSLREYYDERVSGVADEAGESERTI"
BASE COUNT         2216 a         2276 c         2125 g         1937 t
ORIGIN      
        1 cgtagcgcct cacgctttcc aagactccga catcgctgca agcatttttt gaaaagacac
       61 tcaacccaga agagacgtac ttcaagcgca gagagtcaaa tccctgaccc tgggcaatgc
      121 cgaagctaag ctgggaggca taacccaaaa taagaataag caccacattc cagcggcccc
      181 tctgtggggc tcgaagcacc tggcgggaga caaacatcgc caggattaat atcaccagga
      241 agatcagttg tcgaaaactc atcccccgct ggcggaaatt ccaaaaccag tcaggtattt
      301 cttcaaagat agcaatctgc atggacaaga agaccagttg aaaaattgtc actgccagaa
      361 ggctgacaaa cagcgaggcg acaatgatct tgggctgcac gtagagccta cgcaccatcg
      421 cttccgggat cgacaaatac aaaataccca gggtctggac agcggccagg gtagcccaaa
      481 tctgggcagg gtaaagcctc tccaaggcaa tctggagcac atacgggaga tccggaaggc
      541 ggtcggatga tgaaacggat aataatgcca agccaccgat aacaatcatc gtgagggaca
      601 gggcggataa gattaatctc cttgaatcgg aaacaagcag agcgaggttt gagacagcac
      661 ttgccaacca ccgcttatca gtaaacgcct tgaggctcaa ccaacccatg cataaaatca
      721 accccagcat ggccgcccct aacaccaggc gggaggggga aaatccgaac agccaggcat
      781 tacgctccat cgattgggat tgaaaggaat agtaaaaggc gacaatcccc tcgagggttg
      841 tcagtaagac ataaaactgg aagataaccg gccttctaga taaagtcatt tggctatgga
      901 aatatagatc gcacctggat gataaagctt cttctgtatt tgatcaatat atgaattcat
      961 cccaacaaac ttgcgcttat caggagtccc gaaattcgga aggaatttat agcatgatac
     1021 acttataaat attatattag gaaccgaatt ccagtaaatt caaggctatc gagcgcgcaa
     1081 cagcttcccg acatacttct cccaggaccg tagccccaac tctatcgaaa accaaacgag
     1141 agtggcttcc catgacaact attatttttg aaatccttat catcttctta cttattcagc
     1201 tcaatggcgt tctggcgatg tctgaaatcg ccgtaatatc tgcccgtaag gtcagactcc
     1261 aacacagggc tgccgagggc gatgcaggtg cccaagccgc cctagaaatc gccaaccagc
     1321 ccagtcattt tctctcaacc gttcaaatcg gtatcaccct ggtgggtata ctgactggtg
     1381 catttagtgg ggcgaccatc gcagaagaac tagaggccta tttaggtcac acgcagatac
     1441 cactcctgtc ggcatacagt gaggtaatca gtgttagcgt ggtagtgatc gtaattacgt
     1501 ttctgtcgct tattgtgggt gaactagccc ccaaacaaat cggattgaac aacccagaaa
     1561 ggatcgcctc aatcgtcgcc ccaattatgc gaacgatctc acgtctgaca tcgcccttag
     1621 tccacctgct cagtttttca acgagcctgg tcttacgcct catcgggatg aggaggacaa
     1681 ctgatccgga agtcacggag gatgagatca agctgatgat tgagcaagcg acggcagggg
     1741 ggatttttta cccagaagaa gaggagatgg tcgaacaggt tttccgctta gccgaccgcc
     1801 caattgtggc acaaatgaca ccccgtaccg agattgtctg gctggattta gagaatccca
     1861 tcgaagcgat ccgggagacg cttacagccg cccgatattc gtattacccc gtggggcggg
     1921 gtggcgtgga taacttgctg ggtgtggtgc atgcaaaaga cctcctggcg caatgcgtga
     1981 gtgggaaatc tcttaacata gaggaaaaaa taattcaacc ccaatacttg cccgagggca
     2041 tgaccatttt agagactttg aagcggtttc gagagagccg cacccagatc gcctttatca
     2101 tcgacgagtt tggcggaatc caggggctga cgaccatcac caacatcctg gaggcaattg
     2161 ccggcgattt cccggatgcc atcgacgcag ccgaacaaga gatcgtagtc cgaaaggacg
     2221 gtagctacct actgaatgga atgctagaca tcgatgagtt caaagatctg ttccgtatag
     2281 ctgcgctacc aggcgaggaa ggtaattctt atcagacgct cggcgggttt gtgatgatgt
     2341 ttttaggtcg tatcccttcg gcgggagact acttcgaatg ggaaaatctg cgtatcgaaa
     2401 ttgtcgatat ggatggcctt cgggtggata aggtattggt aacacctagc acggtctcac
     2461 tacaataacc catcaagaga ctataggaaa tctaagcttt accccgccat ccccggccag
     2521 cttgcgatgg gatcggtgga gcggacgatg tgcatacccg cttcagcgaa gcgctggaag
     2581 gcttcatcgg ccgatccagt gtagtccacg atatccggca ccaccaccgg cgaggtgcaa
     2641 tcttccaata aatacacctt gtgcgccagg agcggattgt aggcctggat ctggctcaac
     2701 aagtcatcga ctgtccatgc cacacagtgg ctctttgcct ctccggcaat taccacggca
     2761 tcgaattcgt ccaaaaggcc gagaaaacgg ggattcttct ggccgatttg ttcaccgcgc
     2821 cggtcttcaa gcacctctgg gccaatcgca gaatagctct cggaaatggc taactgtcct
     2881 ttggtttcaa tcgctggctg tgcctgccgg gcaatggagt gaaagaagat cgcctcttca
     2941 aaagatgaaa ccaaggcatg accaattccc cccagcatgg catgatatgg ccagatggtc
     3001 agatcgtact tcccctgctc cttaagcacc ttagtatagt gcaacaagtg ctcctgtcca
     3061 taggccgcgt ctatccccaa gattggagcg atagccgcat tgaacctcca tcgtccctgc
     3121 tctacatcct gataagagat taatgagagc ggcgcaggat gctcaccctg ctcgttcacc
     3181 aaaaatatgg ggtggaagat ttgcatagcc tggtgtgtat ccatagtcgg gataatgcga
     3241 gttaataccc ccaggttgcg atagatgaac tcacacaggc ggcgattatc ctcaacagcc
     3301 cccatacctg agcggccagc gacaaacaat tcgaagtccg ggaggcaaaa agtgttctgc
     3361 atatcgactg cgatcagggc aaccctgaag tgatcttcag ctgcgggttg caggttatgc
     3421 tggcgtgccc attgcgaagc cgattctgcc agcgcctggt aaggcactct ccatacttgg
     3481 gctaccttcc ctgggtcgaa gtgcggtggc aatggcaaag ttttaatccg agtggttgat
     3541 ggcagggtat tcatgctacc ttcctgagaa ttcaattagt gtataaacta cactaattat
     3601 accagaatca atgcgcttcg tcaagctaaa tttacgtcat ctaggttaaa aaatcacagc
     3661 ggggttattg cccgctgtga tttttacaga agatattcag ttggtgagga ttagcctttc
     3721 gaccgtttaa gtctcaataa gctgaggtcg ccattcacat tatgcattct gtaccgatgc
     3781 cactggctcc tggctgcgtc gcaagcccac cagaatggca gcgaaatatc caatatatgc
     3841 caacacctca gacagggatg ggttgccatt gtatccgaag agggctttca acatcaaccc
     3901 cagggtggat ttctcgtcta agatgaaatt aatatcccat acatgttcaa ttagggatgg
     3961 gatccaacca agctcattga attcgtggac cccataagcc accaacccgg ctgcaaacaa
     4021 aatcagcaga gcaccggtga tctgaaaaaa tctgcgcaaa tctagccgaa ctgtggatgt
     4081 aaacagcgac cagcctaata aaatcgccgt tcccattccc agcactgcac ccaaaattgt
     4141 gtgctgggcg tttatggcaa acgtggatgc ggtcaggaag agtgctaact caacaccctc
     4201 gcgtaagact gccaggaatg ccaataaaaa caatgcacgc acacccgttt tcgatacggc
     4261 atgacgcaca cccagctcca gctcaccctt gatagtacgg gactggcgat gcatccaaaa
     4321 gatcatccag gttagcacac ccgctgcaat gaacacggtg attccttcaa atacaccctc
     4381 ggctgaaccc tctagcgaag cgccaaccgc atataatgcc agagcgacaa tcacgctgat
     4441 agccgatgcg cttagcgtac cgccccatac cacggggctc aagtcttggc gatgaatttt
     4501 tcgcaaggca cccaaaacaa tgcctataat cagggcagcc tccaagccct cgcgtaatga
     4561 aagaagaaaa ctaggaagca cgacaatcct ccttattagg gattaggtag ttactcatca
     4621 tttgcatggg atgccataca atctggacaa agaccttcga acttatagtg tgcatcgatg
     4681 atacgcacgc catagtggcg ttccatttct tgctgagctt gcgccatcgg tgggctgagc
     4741 aattcaagca ccttcccgca acgaaggcag gcgaagtgat agtgaaatgg cttatcaatt
     4801 aactcgtaat aacccctctt ctgcccttct cccaaaaagc tctgttgaat aagcccaatt
     4861 tgggataaga catttagatt acggtaaatt gtagccaggt tgatatcttg atcatactgg
     4921 cgggcgtttt gccagatccc ctccgcatcc aaatgctcag atgcctcacg tagaatctgc
     4981 aagaccagct ctctttgcgg tgttagccgc aacccgtgat catgcaaggt atcgatgaag
     5041 gtgtcttggt tgtcaggcat agtattggtg ccgcccctta ttgcaaatga tttgcaattg
     5101 cattctatca cagatataat ctctatccac tgatagatgt catcaatgtg ttggtaaatc
     5161 gtcatacttc tcttgtcgac gagatcaaac cccgtttcat caatgattgg ttaggaacaa
     5221 aaatgttttt aagtcgcgtt ctaatcaaat aaaggggtga tacaataata acctggggat
     5281 caccctttat catgcttgcc ttttttgatt atttcgagta aatttataaa tattatgtaa
     5341 atactttgat ctgatactca aagctctaca ttgaaagact gcaaattttt cacaccttta
     5401 tacaccgaat tacccggaaa cgtgatctta ggggagacaa agctcaaatc tctaaacgag
     5461 gttttttgga gaaaacatga ataaatacgc tgacctcgaa atcggtctgc atcgccggga
     5521 attagataca tacagcgttg acttccggtt cagccagccg gatagtgaaa ccgatgtccg
     5581 cccagggcgt ggtgacgctc agatcgacct caaagcgctg caaaaatttc ttccgggaag
     5641 cgccgactac agccaggcgc tcaccagcag cctatttacc gacccagccg tgctgaccgc
     5701 cttctcccag gcggttacca gcgtacagac ttctgggtca gcgctgcgag ttcgtctgct
     5761 catcggctcc agcgctccag agctgcatgg tattcactgg gaaacattac gcaatccgca
     5821 gactgacgcg ccgctttgta ccgacgagaa tatccttttc tcccgctatc tgagtagcct
     5881 ggactggcga ccggtgcacc tgcgtcccaa aggcgatctg aaggctttgg tcgtcgtcgc
     5941 caaccccagc aatctctcgg attatcaaaa tctagccccg gtcgatgtag aaggcgaaac
     6001 cacccgggcg cgagaaaacc taggccccat ccatgtgaca accctccccc cttctccttc
     6061 tgatacagcg ccaggatcca gggcatcgtt gccaaacctg atcgcacgcc tgcgggatga
     6121 gaatccggat attctctatc ttgtggctca tggggcgctc acgcgcgacg agccaaaaat
     6181 atggctggag gatgaagacg gcaaagccgc agtcacttcc ggcaccgaat tggttacacg
     6241 gctgcgcgag ctttccgaaa gacccttgct ggtggttcta gcctcctgcc agtcagctgc
     6301 acatcccaca tatggcgaag cccttactgc actgggtccc cggatggccg aggctggcat
     6361 accagccgta atagccatgc aagccaacgt cagcatgaag accatcgccg agtttatgcc
     6421 cgtctttttc gccgaactgc agaaagatgg gcagatcgac cgcgctttgt ccgtagcgcg
     6481 cggaacagtg cgccagcgcc ccgattactg gatgccagcc ttatttatgc gactgaagag
     6541 cgggcgtatt tggtacacgc ccggttttgg ggaagagcgc ggagcttttg agaaatggcc
     6601 ttccatcatc cgcaccatca gccgaggcct gagtaccccg atcctcggac cgggattgta
     6661 tgaaactgtg cttggctcgc cgcgcgagat tgcccggcgc tgggcggaag tttaccacta
     6721 tccgatggag ccacacgagc gcgagtccct accccaggta gcccaatttc ttgccgtaaa
     6781 ccaatacgac ctggcgcctt tcgacgagct ggaagaatat ctcaagaagg atatacgaag
     6841 ccggtttggc gacaacttac cagacgagct gcgcgctaac ggcgcctctc tggaccagct
     6901 tatcgaggca gctggggcca ttcgccgaca acaatcggag gtagaaccac ataaagtgct
     6961 cgcctccctg cccctcccgg tttacatcac aaccaacctg aataacctga tgggctcagc
     7021 cctaaaggaa gcgggcaaag agccccagat cgtgctctgc ccgtggaatg attacgtcga
     7081 agagctccaa gcagactttg atccaggtta caagccgacc gtggagcgcc cgctggtcta
     7141 ccatcttttt ggtcggttaa gcgaaccaga atctgtggtg cttaccgaag atcactattt
     7201 cgattatctc attggggtta cccgcaacaa ggaaatcatc cccagcgtgg tgcgcagtgc
     7261 cctggttaat tcggcgctct tgttcctagg atttcaactc gacgactggc aatttcgcgt
     7321 actcttccgc agtgtacgcg cccaacaggg tggtgaattg ctcggacgtt acccgcatat
     7381 cgctgtgcaa cttgaacccg aggaaggccg cctattagaa cccgagcgcg cccgaaaata
     7441 tttagaaagc tatttttctg ctggctccaa catcagtctg ttttgggggc gagccgaaga
     7501 ctttgtcaaa gaactacatt cgcgctggaa ggcgcgttca gcctgagatg aagccatgac
     7561 aataacgaat acccgtccta acccatatgt tggtccgaga gcctttcgca caggcgaagc
     7621 gctttatggt cgcggtcgcg aactgcacga gctcttgaac ttgctcatcg ccgagcggat
     7681 tgtcttatta cattcaccct ctggagcagg caaaagctcg ctggtgtatg ccggcttgat
     7741 cccccgtctc gaggaggagg ggttccaggt gctacctgtc gcgcgcgtta accaggacct
     7801 gcccgcatcg ctcggcagcg atgagcgctt caatcgctat gcctttagca ccgttgtctc
     7861 gctggacgag gctctgccag agaagaagcg gcttccttta gaggaattgg cgaccatctc
     7921 attgcccgat tacttggagc gtttcaaggc tcaaaactta accactgttg gtgatacaga
     7981 cgatctgcca cccatggaag ccgaggtgtt gatatttgat cagtttgaag aggtgctgac
     8041 tgtcgccccc actgacctag aaggcaaagc cgcctttttt gcacagctag gtgcagcctt
     8101 acgcgatcga aaacgctggg cgcttttcgt tatgcgcgag gattacatcg ctgccctcga
     8161 tacttatctg cgcctggttc ccacgcgttt ccggaacact tttcggctgg acctattggg
     8221 agtggaagct gctatgcaag ccatccagtt acctcccaag gaagctggcg tgaaattcaa
     8281 caataatgcc gcccagaagc tggtggacga cctgcgccag gtgcaggtgc agcgccctga
     8341 tgggaccatg gagatccaac caggtccaca tgtcgaacca gtgcaattgc aggtagtttg
     8401 cttccgccta tggcaaaacc tggaactaga tgacaagcaa atcagcatcg aagatgtggt
     8461 cagcgtcggg gatgttaatc aatctttgag ggaatactat gacgagcgcg tgagtggcgt
     8521 agcggacgag gctggggaaa gcgagcgcac tatt
//
LOCUS       DBNGSX010000566         2157 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_473448, whole genome shotgun sequence.
ACCESSION   DBNGSX010000566 DBNGSX010000000
VERSION     DBNGSX010000566.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_473448"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="AC16CB_16595"
     CDS             <1..848
                     /locus_tag="AC16CB_16595"
                     /inference="COORDINATES: protein motif:HMM:NF014799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HIR3344143.1"
                     /translation="EIMFGDFVALAADQFVNHLAKFRWMYNEQVRLPLVIRTPMGGRR
                     GYGPTHSQTLEKLYLGLPGLRVIAPTALGNPGDLLYDTILHTGDPVLFIENKLLYAMP
                     LHGEDLVEDFIPYPLSPIPSEIGGERQGIGDHSNPTYGLSLRGAPPPQLTITAYGYMA
                     ELALQAVRRLAYQHEIFVELIVPTELSAFDLETLCSSLERTRRLLTVEEGTYTLGWGA
                     EVVARAAEKLGPRLHASRRLAAQDLPVPASGPLEAAVLPGVDEIISAALSLSTGKDRK
                     HDLSR"
     gene            complement(1082..1687)
                     /locus_tag="AC16CB_16600"
     CDS             complement(1082..1687)
                     /locus_tag="AC16CB_16600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR3344144.1"
                     /translation="MNPGANSKELTRMARWLAILLVLSLLVSMLPQGVLAAPLDAKDD
                     CKRSYEVTRTDTLKKIARRFGVSVATIVAENDMKKPYTIYIGQRLCIPKRTKKGLSNL
                     PSKNTNTYAVFFVASRAGNDIIIYTYNYPKTTVLVKGENAGDSKWKLVDIGKINIATQ
                     GNRKAIRFKLPSELRVKKLMICLKDKSSGNLQCVYQRFSSK"
     gene            complement(1813..>2157)
                     /locus_tag="AC16CB_16605"
     CDS             complement(1813..>2157)
                     /locus_tag="AC16CB_16605"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3344145.1"
                     /translation="VFLAREGFYDGVTFHRVIANFVIQGGDPTGTGRGGPGYRFEDET
                     RGNPLRHETNVISMANAGPNTNGSQFFITYSPQPHLDGKHTVFGKVVAGNDVVNAIEQ
                     GDQMVKVEVEES"
BASE COUNT          454 a          608 c          558 g          537 t
ORIGIN      
        1 tggagatcat gtttggcgac tttgtcgctt tggctgccga ccaatttgtt aaccacctcg
       61 ccaagttccg ctggatgtac aatgagcagg tgcgcttgcc tttggtgatc cgcaccccga
      121 tgggtgggcg gcgcggctat ggacccaccc acagccaaac gctggagaag ctttacctgg
      181 ggttgcccgg cttgcgggtg atagctccta cggcactagg aaatccgggc gacttactct
      241 acgatacgat actgcacacc ggagacccgg tattattcat tgaaaataag ctgctgtacg
      301 ccatgccgct ccacggcgaa gatttggtcg aggattttat cccttatccc ttatccccta
      361 tcccatcaga gattggtggt gagaggcagg gaatagggga tcattccaat cccacctatg
      421 ggctctcctt gcgcggggcg ccgccccctc aattgactat caccgcatac ggttacatgg
      481 ctgagctagc cttacaagcc gtccgccgct tggcttacca gcatgagatt tttgtcgagc
      541 tgatcgtccc cacagagcta tcagctttcg acctcgaaac gctgtgctcc tctctcgagc
      601 gcacgcgcag gttgttaacc gtagaagaag gcacctacac cttgggatgg ggagcagagg
      661 tcgttgcacg cgccgctgag aagcttggcc cgcgcttaca tgcctcccgt cgactggcag
      721 cccaggatct gcccgtaccc gcctcaggac ctttggaagc tgccgttctg cctggtgtgg
      781 atgagatcat cagcgccgcc ttgagcctct ccacgggtaa agatcggaaa catgacctat
      841 cccggtaata gggggatgag aaccgaaaaa aagtcctgtt tgtgggccga ggcccacaaa
      901 caggactttt tttctatcaa tcttcctccc ccgagtttgg gggagggtcg gggtgggggt
      961 tgaagtatga aattaacccc cagaaggatt gaatttcggg tgtttcatgc ctcttctccc
     1021 tgccccttga ggagtggcct acccaggggt cttggaagaa actccgcagt aaagtgtgct
     1081 gctacttcga gctaaatctc tggtaaacgc actgcaggtt gccagacgat ttgtctttca
     1141 ggcaaatcat cagctttttg accctcagct ccgaagggag tttgaagcgg atcgccttgc
     1201 gattgccttg cgtggcgata ttgatcttgc cgatatcgac cagtttccat ttgctgtccc
     1261 cggcattctc gcctttgacc aggacagtgg tcttggggta attgtacgta tagatgatga
     1321 tatcgttccc cgcccggctg gcaacgaaga aaaccgcgta ggtgttggtg tttttgctcg
     1381 gcagattaga tagacccttc ttcgtcctct tgggaatgca caacctctgg ccgatataga
     1441 ttgtataggg cttcttcata tcattctctg caacgattgt cgccacagac acgccaaaac
     1501 gcctggcaat ttttttgagc gtatcggttc gtgtcacttc gtatgaacgc ttacaatcgt
     1561 ccttcgcatc cagcggagcc gctaaaacac cttgcggcag catagagacc agcaggctga
     1621 gcaccaacaa tatcgccagc cagcgagcca tcctggttaa ctcctttgaa tttgcgcctg
     1681 ggttcatgat gcattctcct ttcctactca aaaaatacgc ccaaagcgga ggcacctgaa
     1741 ttcattatac aataaatttc cgcttgtagt ggtggacgca agtacacccg ctcctatgcc
     1801 cccttttttc ccctaactct cctcaacttc cactttcacc atttgatctc cttgctcgat
     1861 cgcattgacg acatcattcc ctgcgaccac ctttccaaac accgtgtgtt tgccgtccag
     1921 gtgcggctgg ggggagtagg tgataaagaa ctggctgccg ttggtgttcg gtccagcgtt
     1981 agccatggag atcacattgg tctcgtggcg cagcgggttg ccacgcgtct catcctcgaa
     2041 tcgatatccg gggccaccgc gcccggtccc ggttgggtcg cctccttgaa tgacaaaatt
     2101 ggcgatcacg cggtggaagg tcacaccatc atagaagccc tcgcgggcta aaaagac
//
LOCUS       DBNGSX010000567         3064 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_58786, whole genome shotgun sequence.
ACCESSION   DBNGSX010000567 DBNGSX010000000
VERSION     DBNGSX010000567.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_58786"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="AC16CB_16610"
     CDS             <1..764
                     /locus_tag="AC16CB_16610"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3344146.1"
                     /translation="GEISEIVLNFERPGKYVFYCSRWCSVNHWRMRGTIEVSGAAQAM
                     PDDAEPPLYVQLGLDIDEEHHAEVLPERMPSAAQGKALLASLPDNAIPTIYLGPDTYY
                     TNSPQQLWQALRAEQGLSGLADQEVWDVVAAIWESNTTPQALAEGQAMYSANCAACHG
                     EGGAGDGVFAAQLTEHPSDHEEMQADQMSQPPTDFTDPEHLLAASPAHLQGKIIRGGM
                     GTGMPYWGPIFTEEQTWNLVAYLWKFQFNYEETEL"
     gene            761..1075
                     /locus_tag="AC16CB_16615"
     CDS             761..1075
                     /locus_tag="AC16CB_16615"
                     /inference="COORDINATES: protein motif:HMM:NF046853.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR3344147.1"
                     /translation="MSQTKSKIVKKRKRQNRSLVPLLLGLGGVVLVALAGIFIAMGGG
                     GKKASIVVQGAPSLKVDKEKLDFGDVKLGNWVEAEFTLTNVGDQPLRFTDQPYIEVVE
                     GC"
     gene            1295..1609
                     /locus_tag="AC16CB_16620"
     CDS             1295..1609
                     /locus_tag="AC16CB_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344148.1"
                     /translation="MILDKPCDHLVDWAVQKLTNAGLQVMPTFNLQAARLSHPDCPCP
                     YHGTQACDCQMVVLLVYTDEPQPASLVIHMHHGRALFYLVDTPQQRAGEHLIALIQGA
                     LL"
     gene            1710..2369
                     /locus_tag="AC16CB_16625"
     CDS             1710..2369
                     /locus_tag="AC16CB_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344149.1"
                     /translation="MSLIGVVYTGLPFLAPLLMHIGWTSAGSAIYTIYSLLCHQLPQR
                     SFFLFGNRFTYSLIEIQSVWENSINPLVLRKFIGNSEMGWKVAWSDRMVWMYVSILFF
                     GLLWWLLRYRSKPLPWQGLILFLLPIVLDGTTHFFSDLAGIGQGFRNDNVWLRILTRE
                     AFSISFYTGDAWGSFNSLARLVTGILFGMGIAWFGYPYLNEFFVQISNSVTFEGGAKG
                     G"
     gene            2460..>3064
                     /locus_tag="AC16CB_16630"
     CDS             2460..>3064
                     /locus_tag="AC16CB_16630"
                     /inference="COORDINATES: protein motif:HMM:NF045289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344150.1"
                     /translation="MELMFLGLTSAQWTEAGISLLIVIAVVILGRRLVAIVLDLGIKR
                     LTSSTKTNFDDAILNTLRLPSYWLIVVLSLDYALRRLDFITERWGEQTDSLLYILYLL
                     VGFAFAWSLIQNMFAWYGEEIATRTETDLDQQLMPFFRRVALIILTAVGLITLLGYFN
                     VEIGALVTTLGIGSLAIALAAQAALSDTISGFIIMIDRPFR"
BASE COUNT          684 a          766 c          837 g          777 t
ORIGIN      
        1 ccggtgagat cagcgaaatc gtgctgaatt ttgagcgacc gggaaaatac gttttctact
       61 gctcgcgctg gtgcagcgtc aatcactggc gcatgcgcgg gacgattgaa gtaagcggag
      121 cggcgcaagc catgccggac gatgccgaac cgcctctgta cgtgcagctg ggactggaca
      181 ttgacgagga gcaccatgct gaagtcttgc cagagcgaat gccttcggca gcccagggga
      241 aggcattgct cgccagcctg cctgataatg ccatacccac catatacctg gggccagata
      301 cctactacac caacagcccg caacagctgt ggcaagcgct cagggctgaa cagggattga
      361 gcggtctcgc tgaccaggaa gtttgggatg tggtggcggc gatctgggag tccaacacaa
      421 ccccgcaagc gctggcggaa gggcaagcca tgtattctgc caactgtgct gcctgtcatg
      481 gggagggagg cgcaggcgat ggagtgtttg ccgcccaact caccgaacac ccgagcgatc
      541 atgaagagat gcaagcagac cagatgtccc agccgccgac tgattttacc gaccctgagc
      601 atctactggc tgccagccca gctcacctgc aaggcaagat catccgtggg ggaatgggca
      661 ccggtatgcc ctactgggga ccaatcttca ctgaagagca gacctggaac ctggtcgctt
      721 atttatggaa attccagttc aattatgagg agaccgaact atgagccaaa ccaaaagcaa
      781 aatcgtgaaa aaacgtaaaa gacaaaaccg cagccttgtg ccgctcttgt tgggattggg
      841 aggcgtggtg ctggtggcgc tggcgggtat attcatcgcc atgggcggag gcggcaagaa
      901 agcctcaatt gtcgtccaag gcgctcccag tctaaaggtg gacaaggaga aactcgactt
      961 tggtgatgtc aagctgggta actgggtgga agcagaattt acgctcacca atgtgggtga
     1021 ccaaccctta cggttcaccg accaaccgta catcgaggtc gttgaagggt gttgaccccc
     1081 caaactagtc atcggtgcga tgaccctcaa tcctggtgaa aacaccaccc tgtctatgga
     1141 atttatgatg catggtgata tggacggaat gcacgatttt cgggtccatg tagccagcaa
     1201 cgaccctcag cagccggagc gtaccctgac tgtactgtcc aactgggtgc cataattcct
     1261 taattgaaaa cgcatgattg gtgcctcgcc ttttttgatc ttagataaac cctgcgatca
     1321 cctggtcgac tgggctgtcc agaagctgac caacgctggc ttgcaggtta tgcctacttt
     1381 taacctgcaa gcggcgcggc tcagccatcc ggattgcccc tgcccttatc atggtacaca
     1441 ggcgtgtgac tgccagatgg tggtgctgct cgtgtataca gatgaacccc agccagccag
     1501 tttggtcatc cacatgcacc acggtcgcgc cctgttttat ctggtcgata caccccagca
     1561 gcgcgccggt gagcatctca ttgccttgat ccagggtgct ttgctctagg accgggaggt
     1621 gagttgcagc attgagcgcg caatctatac gctaccgatc aattcttggg cgcctggtaa
     1681 atgctttttg caggcgctgg atattttggg tgagtctaat cggggtggtt tatacagggt
     1741 tgcctttcct ggcgccgctt ctgatgcaca tcgggtggac aagcgcgggt agtgcaatct
     1801 acactatata ttcactcctt tgccaccaat tgccacaacg ttcgtttttc ttgtttggca
     1861 accgatttac ctattccctg attgaaattc aatctgtttg ggagaatagc atcaatccat
     1921 tggtgttgcg caagtttatc ggtaattcgg agatgggctg gaaagttgcc tggtcagatc
     1981 gcatggtatg gatgtacgtc agcatcctat tttttggttt gctctggtgg ttgcttcggt
     2041 atcgatcaaa acccttgcct tggcaaggac ttattctttt ccttcttccg attgtgttag
     2101 atgggacaac gcactttttc agcgatttag ctgggatcgg ccaagggttt cgcaacgaca
     2161 atgtgtggct tagaatccta acccgcgagg cgttctcgat tagcttctat acaggagatg
     2221 cctggggatc gttcaactca ctggcgcgcc tagtaaccgg gattctgttt gggatgggaa
     2281 tagcttggtt cggctatccg tacttgaacg aatttttcgt gcagatttca aactcggtaa
     2341 ctttcgaagg aggcgcaaaa ggggggtagc tggctttata atattcttca ttgggttttt
     2401 gttaaagcga gaagacctgt aagattttcc ttttgatata atctctgcag gagtttggta
     2461 tggaattaat gtttctagga ttaaccagtg cccaatggac cgaagccggg atatctttgc
     2521 tcatcgtcat tgcggtcgtg atcttgggcc gcaggttggt ggcgatcgtg cttgacctag
     2581 gtatcaagag gttaacctcc agtacaaaga cgaacttcga tgacgccatt ctcaacaccc
     2641 tgcgcctgcc gtcttactgg ctgatagtgg tcttatccct tgattatgcc ctgcgtaggc
     2701 tggactttat taccgagcgg tggggcgaac agacagactc gctcctctac attctttatc
     2761 tgttggtggg atttgccttt gcctggagct taatccagaa tatgtttgcc tggtacggag
     2821 aggagattgc cacacgcacc gaaacagatc tcgatcaaca attaatgcct ttcttccggc
     2881 gggttgcgct gatcatcttg accgcggtag ggttgatcac tttattgggt tatttcaatg
     2941 tagaaatcgg cgcgttggtc actaccctcg ggattggatc actcgccatc gcgctggcgg
     3001 ctcaggcagc tttatctgac acgatcagcg gattcatcat catgatcgac cgacccttcc
     3061 ggat
//
LOCUS       DBNGSX010000568         2079 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1858548, whole genome shotgun sequence.
ACCESSION   DBNGSX010000568 DBNGSX010000000
VERSION     DBNGSX010000568.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1858548"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..919
                     /locus_tag="AC16CB_16635"
     CDS             293..919
                     /locus_tag="AC16CB_16635"
                     /inference="COORDINATES: protein motif:HMM:NF024248.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3344151.1"
                     /translation="MENFTPSPVMILEDLETLKVLADPFRNQILEVLAPGPLTVNQIA
                     EKLGLTPSKLYYHINMMEKHNLIKIIDTTVKANIIEKHYWIMAYEFKLAEALCNFSTP
                     EGQNSVTTTMVAPIETTLGDIKRSLKARAYALDHGAKQHPREVVIYRELRSIPDELAT
                     KFIARLNEVMDEFNAYENQERSESVQTHALTIAFYPSFYFENPNKQDD"
     gene            1027..>2079
                     /locus_tag="AC16CB_16640"
     CDS             1027..>2079
                     /locus_tag="AC16CB_16640"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3344152.1"
                     /translation="MKGFQIVIRMRTFLIIWFGQLISIIGSGLTGFGLSVWIFEKTGQ
                     ALPIALNALFFNLPRVLLAPIAGAIADRYNRRHIMILADSGAAVTTLGVVALLFTGRL
                     EVWHIYLSTAISSCFGAFQDPAYRASITMLVPKDDLARAGGLDQMSFAVQSILTPLLA
                     GVLYAGFGLKAIILIDFVTFFFAVGALALVHIPQPKATSVAAEDRQRNRMWREAAFGW
                     KYLLERPGLFGLLWYYAVVNFFLTLSGVLFAPLVLSYGTPTEMGVVQTFGGVAMLVGG
                     LIMSIWGGPKSRRIWGVIAAIALSGFGYLLAGLRPSTLLIASAQFVILFFIPISAAIS
                     QAVWQMKVAPEIQGRVF"
BASE COUNT          514 a          515 c          507 g          543 t
ORIGIN      
        1 cgcagcccag tagggtgaat aagtagaaaa ttgcatcgcg cagtttgagc cggtccactt
       61 cgaggatgca caaaggaccg cttgacctag gtttcaagcg gtccttgtag ttaatccatt
      121 ttgaccagca aagccccaaa aggtgttagc gctgcacctg agtgtggtaa ttctcttccc
      181 cttccattaa accctgatag agtttaaccc actcctcttg acaataatat attcttgcga
      241 tataaattat tttatcagtt aaatattttt taacgccaaa aaggacatga atatggagaa
      301 ttttacgcca tcccctgtca tgattttaga agacttggaa accctaaaag tcttggctga
      361 cccttttcgc aatcaaatcc ttgaggtcct tgcaccggga cctttgactg tgaatcaaat
      421 tgctgaaaaa ttgggactta ctcccagcaa gctttactac catataaata tgatggaaaa
      481 gcacaatttg atcaagatca tcgacaccac cgtgaaggcc aatatcatcg agaaacatta
      541 ttggattatg gcttacgaat tcaagttagc tgaagcactg tgcaactttt ctacccccga
      601 aggtcaaaat tctgtaacca caaccatggt tgctccaatt gagacgacct tgggagacat
      661 caaacgcagt ttgaaagcac gagcatatgc gcttgatcac ggcgctaaac aacatccacg
      721 cgaggtggtc atctatcgag aacttcgcag catcccagat gagcttgcca ccaagtttat
      781 tgcccgcttg aacgaggtca tggatgaatt caatgcatat gaaaaccagg aacgctcaga
      841 aagcgtccag actcatgcac tgaccattgc cttttacccc agtttttact ttgaaaatcc
      901 caacaagcaa gacgattaga tatttctgca acagatgaag agcattgtat aactcccaac
      961 gagccagcac gggcgtatac ctaagcaaat actaacgacc taggtcgcac ccaaaggagc
     1021 agagacgtga agggtttcca aatcgtgata cgaatgagaa cattcttgat catctggttc
     1081 gggcaattga tctcgattat aggatctggc ctgacaggct ttggcttgag cgtgtggatt
     1141 tttgaaaaga ccgggcaggc attgccaatc gccctcaacg cattgttttt taatttacca
     1201 agggtgctgt tagcccccat tgcaggcgca attgcagacc gttacaaccg gcgccacatc
     1261 atgatccttg ccgatagcgg tgctgcggtg accaccctcg gggtggtggc actgctcttc
     1321 accggcagat tggaagtatg gcatatctac ctgtccaccg ctattagctc ttgcttcggt
     1381 gcattccagg atccggcata tcgggcttca atcaccatgc tggtgccgaa ggacgatctg
     1441 gctcgcgctg gcggattgga tcagatgagc ttcgcggttc agtcgatact cactccattg
     1501 cttgcgggcg tgctatacgc cgggtttggc ttgaaggcga tcatcctgat cgattttgtg
     1561 acgttcttct tcgctgtcgg cgccctggca ctggtgcaca tcccgcaacc caaggcaaca
     1621 agcgtagcag cggaagacag gcaaaggaac aggatgtggc gcgaggcagc cttcgggtgg
     1681 aaatatctgc tcgagcgccc tgggcttttc ggtcttctgt ggtactacgc agttgtcaat
     1741 ttctttttga ccctttctgg cgtacttttc gcgccgcttg ttctctcata cggcaccccg
     1801 accgagatgg gtgtggtcca gacgttcggg ggagtggcta tgttggtcgg gggcttgatc
     1861 atgagtattt ggggagggcc aaagagccgc cgtatttggg gtgttatcgc agccatcgcg
     1921 ctgagcggtt ttggctatct cctcgcagga ttaaggccgt ctacattgtt gatcgcttcc
     1981 gctcaatttg tgatcctgtt cttcataccc atatctgcgg cgatcagcca ggcggtttgg
     2041 caaatgaaag tagcacccga aatccagggt cgcgttttt
//
LOCUS       DBNGSX010000569         3241 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1305300, whole genome shotgun sequence.
ACCESSION   DBNGSX010000569 DBNGSX010000000
VERSION     DBNGSX010000569.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1305300"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="AC16CB_16645"
     CDS             <1..363
                     /locus_tag="AC16CB_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344153.1"
                     /translation="ENTTWDLPCNNSSLTVKNSLLQRAWPNTYGYVHLIISDSFLVDT
                     RNYGAPATMEIYNSTIDIVAAYKGGKVYIENSPVKEAIEVKDSRSVIYGFGVSGEYQL
                     LESDGGIYIELENAEPPW"
     gene            574..1359
                     /locus_tag="AC16CB_16650"
     CDS             574..1359
                     /locus_tag="AC16CB_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020001884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HIR3344154.1"
                     /translation="MQLPIFQVDAFTDKVFGGNPAAVCPLEHWLPDDVMQKIAIENSV
                     AETAFFIPLNDGFEIRWFTPEIEMDLCGHATLATAHVIARHLNYSLPSIKFLSKSGEL
                     MVTVENELLTLNFPSRKPEPSDIPQIILDAIQVKPVEVLKSRDYVLVFETEEIIRRIE
                     PNQSILNQINLDPGGVVITAKGDEVDFVSRYFTPQASIFEDPVTGSAHCSLIPFWSEK
                     LGKESMLALQVSPRVGKLFCKSLGERVLISGEAVTYLEGSITI"
     gene            1440..2060
                     /locus_tag="AC16CB_16655"
     CDS             1440..2060
                     /locus_tag="AC16CB_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344155.1"
                     /translation="MLILAPIKQHYEPKRRQVTQTVRRPEERMPNTEKTMQGHYFQTL
                     SNIAVILGIVFVGWQLYQDREMKRAELITSTLQDEYARAIAIMGENPSITLAKLAEDA
                     ELDSNERIIVNSFFYTQHLTWLRKSILEEMGMLKDGWRADIRLPPSLWANKQGIAYLQ
                     SMADNEFNLEGFDDQIRSEIARLESIYNEKSPPNRQNEYWSWPPTK"
     gene            2200..3000
                     /locus_tag="AC16CB_16660"
     CDS             2200..3000
                     /locus_tag="AC16CB_16660"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3344156.1"
                     /translation="MESVISNDGTRIAFYRGGIGAPLVLIHGTGGSNPAVGWTSVVPL
                     LEKQFSVYAVDRRGRGNSSDNPIYAVEREFEDVAAVVDSIGEPVNLLGHSYGGLCALE
                     ATLITGNVRKLILYEPAVNLSLPASAGPDQITEKIQRLLDEGNRDDALILFYSKVAMM
                     QPEEIEQMKMSPSWAGRIMSAHTLSREMRAHDQYVFNTERFKNMKIPTLLLVGGDSPE
                     SLLGSTNALASVLVNCQVKVMPGQQHVAMLTAPEMFADLVAQFILESV"
BASE COUNT          972 a          708 c          732 g          829 t
ORIGIN      
        1 gaaaatacaa cctgggattt gccttgcaac aattcgtcgc taactgtaaa aaactctctt
       61 ttacaaaggg catggcccaa cacttatgga tatgttcatt tgataatatc agattcgttt
      121 ttagttgaca ctaggaatta tggagcgcca gcgacaatgg aaatatataa ctcaactatt
      181 gatattgttg ccgcctacaa ggggggcaaa gtttatattg aaaactctcc agttaaggag
      241 gcgattgaag taaaagattc acgatcagtc atatacggct ttggcgtttc tggagagtat
      301 caacttctcg aatcagatgg aggaatttat atagagcttg agaatgcaga acccccttgg
      361 taataccaaa aaaaagtacg ccacccaaca acgcgtgcac cccgacaggt gggattcgca
      421 ccgccaaaag gagttaattc tgtgctcgaa cagtttccgt tatttcaacg ttatctcgtc
      481 ccgcccacct gcgggtaacc cagccgttag cccgcccttc aatgtccaaa taagcaatat
      541 cccaaaaatt atctcttaat ctgtaaggtc attatgcaat taccaatatt ccaagttgac
      601 gctttcacag acaaggtttt cggtggcaat cctgccgccg tttgccccct tgaacattgg
      661 cttcctgacg atgttatgca aaaaatcgca atagagaatt ctgttgccga aaccgcattt
      721 ttcattcctt tgaatgatgg gtttgaaatt cgttggttca ctccagaaat tgaaatggat
      781 ttgtgcggac acgcaacact tgcgacagcg cacgttatcg caaggcatct caactactct
      841 ctgccatcta taaagtttct gtcaaaaagc ggtgaactga tggtgacagt tgaaaatgaa
      901 ttattgactt taaatttccc atctcgtaaa cccgaaccaa gcgatattcc tcaaataatt
      961 ttagacgcaa ttcaagtaaa gcctgtcgaa gtgctaaaat ctagagacta tgtgcttgta
     1021 tttgaaacag aagaaataat acgacgcatt gaacccaacc aaagtattct taatcaaata
     1081 aatctcgacc ctggcggtgt tgttataaca gccaaaggcg atgaagttga ttttgtttcg
     1141 cgctacttta caccacaagc aagtattttt gaagacccag taacaggctc ggctcattgt
     1201 tcgctgattc ctttttggag tgaaaaactt gggaaagagt ctatgcttgc actgcaagta
     1261 tcaccaagag ttggaaaatt attctgtaaa agcctaggcg aacgagtctt gatttcagga
     1321 gaagcagtaa cttatttaga aggaagtatt acaatttaga agtccgtgat taaagatagg
     1381 cagcgggcta acacagcgtg caccatgacc tgcttcacgg aggaggaata gggcgcgaaa
     1441 tgctaatact ggctcctatt aagcagcatt atgagccaaa acgcaggcag gtaacgcaaa
     1501 ccgttagacg accagaggaa cgtatgccaa atactgagaa gacaatgcaa ggacactatt
     1561 ttcaaacgct ttcgaacatc gccgtcattt tagggattgt gttcgttggc tggcagctct
     1621 accaagatcg agagatgaaa agagccgagc taatcacatc aacactacaa gatgaatatg
     1681 cccgtgccat agcaatcatg ggcgaaaacc cctcaataac cctagccaaa ctagctgaag
     1741 acgcagaact tgattcaaat gaaagaatca tcgtcaactc ctttttttac acacaacatt
     1801 tgacttggct aaggaaatca atcttagaag agatgggcat gttgaaagat ggctggcggg
     1861 cagatatcag gcttcctcca tcgttatggg ctaacaaaca gggtatcgcg tatttgcaga
     1921 gcatggcgga taacgagttc aacctagagg gatttgatga tcagatccga tcagaaatag
     1981 caagactgga gtcgatctac aatgaaaagt cgccgcctaa ccgccaaaac gagtattgga
     2041 gctggccgcc taccaagtaa ttggagctga ccgccaatac gtcacttcgt ttgcacttcg
     2101 ttcgctgcgc tcgcggcaaa atcgtgccgc aagtatggca gcagctcaac atgatgttgg
     2161 gcggctaatg cctatcaagg attaaggaga ataaaccgta tggagagtgt catttcaaat
     2221 gatggtactc gtatcgcttt ttatcgaggg ggcattggag caccacttgt attgatacac
     2281 ggcacgggcg gttcaaatcc agcggttggt tggacatctg ttgtaccctt acttgagaaa
     2341 caatttagtg tttacgctgt cgatagacgg ggacgtggta atagcagtga caaccccatc
     2401 tacgccgttg agcgcgaatt tgaagacgtt gcagcagttg tagattcaat tggagaacct
     2461 gtaaatttat tgggacactc ctacggtggc ttatgcgccc tcgaagcaac attaatcaca
     2521 ggaaatgttc gcaaacttat cctttatgaa cctgctgtaa acctgtccct ccctgcgagt
     2581 gccggtcccg atcaaattac agaaaaaata caaaggttat tagacgaagg taatcgagat
     2641 gatgctctaa tattgttcta cagcaaagtt gctatgatgc agcctgaaga gatcgaacaa
     2701 atgaaaatgt caccttcatg ggcaggacgc attatgtcag cacatacgtt gtcaagggaa
     2761 atgcgagcgc atgaccaata tgtatttaac actgaacgat ttaagaatat gaagattcca
     2821 actttattgc ttgttggagg agatagccca gaatctctct tagggagcac gaatgcattg
     2881 gcatcagtct tagtgaactg tcaagtaaag gttatgccag ggcaacaaca tgtcgctatg
     2941 ttgaccgctc cagaaatgtt tgccgattta gtggcacaat ttatacttga gtctgtctag
     3001 tgtatggtct agccgcccaa caaagcgttc tcagaaacag cttatgcagg ggatagctgg
     3061 aaaccaagtt cggcagcctt acgctccagg taaactttgt ggacgatttt ctacatggag
     3121 tgaagtatcc ggtgatactc aaatcagatg acaaccaatc catagcacaa cgccgcccaa
     3181 catggcatgc aaccgaccgc ttcgcgctac gcgctcgcgg cggctgatgc gtggcgttag
     3241 g
//
LOCUS       DBNGSX010000570         2013 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1650901, whole genome shotgun sequence.
ACCESSION   DBNGSX010000570 DBNGSX010000000
VERSION     DBNGSX010000570.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1650901"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1761
                     /locus_tag="AC16CB_16665"
     CDS             <1..1761
                     /locus_tag="AC16CB_16665"
                     /inference="COORDINATES: protein motif:HMM:NF013720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3344157.1"
                     /translation="EHQYLISEPLEELKGETREIVHPILRHQDFSMYFRQHKDAYGIG
                     SYKHDPLLVSPYDVGKDAMRPFTASDFKMARTATNELLPALKGKDYLTKFNGMFAFTV
                     DGYPIIGPAPNFQNFWVAVGVWVTHSGGVGRAIAHWMDSGQPNTDVHEADINRFHNYA
                     TSNYYIRRTAAQQYREVYDIIHPLQQMEHTRDVRLAPYHSRLTQLGGHFFQSVGWERP
                     QWYESNRRLLEKYRQQIPEREGWEAMYWSPLQGVEHLATRENVGLFELSSFFKIEVSG
                     PGAADYLEYLTANQVAKDLGKVVYTSLLTESGGIKSDLTVTRLAEDRFWLLTGGGSGM
                     IDLDWVNQHAPKDGSVHIADISSRYTAIGLWGPKARQVLESVAEQDVSNHAFPYFTAQ
                     ELMLEAIPSLAFRLSYVGELGWEIYTPTEFGQRLWDILWNAGQESQMIAAGMGAFDSL
                     RLEKGYRALGNELHVEYNPLEAGLGWAVNLDQGNFLGREAFLKIKQSGVDKMLCCLTL
                     DEGTALGKEPIFTGDKCIGYVTSANYGYSVGKHIAYGYLPAQYARKGTQVEIEYFGER
                     FTAHVDNDPLYDPGMKKLKA"
     gene            1776..>2013
                     /locus_tag="AC16CB_16670"
     CDS             1776..>2013
                     /locus_tag="AC16CB_16670"
                     /inference="COORDINATES: protein motif:HMM:NF025142.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HIR3344158.1"
                     /translation="MAKYKVLYWYDIPTQVRSEDENGRASLQLSDRFQLAIDEAAMAA
                     KLISTDDYTAGFIWHPEQEKEGSAKDVAAVVVAEI"
BASE COUNT          538 a          469 c          533 g          473 t
ORIGIN      
        1 gagcaccaat acctgatctc tgaaccactg gaggaactaa aaggggaaac acgcgaaatt
       61 gtgcatccaa tccttcgcca ccaggatttt tcgatgtatt tccgccaaca taaagacgcg
      121 tatgggattg gctcttataa acacgatccc ttattggtca gcccttacga cgtagggaag
      181 gatgctatgc gtccatttac ggcgagtgat tttaagatgg caagaacagc aacaaatgag
      241 cttttacctg ccttgaaagg gaaagattac ctcacaaagt tcaacggtat gtttgctttc
      301 actgtagatg gttacccgat catcggtcct gctcccaatt tccaaaattt ctgggtggca
      361 gtgggggttt gggtgacaca ttctggaggg gtcggccgtg ccattgccca ttggatggac
      421 agcggacagc ccaatacaga tgtgcacgaa gcagatatca accgcttcca taattacgcc
      481 accagcaatt attatattcg ccggacggca gcccaacaat accgcgaagt atacgatatc
      541 atccacccat tgcagcaaat ggaacacacc cgcgatgtcc ggttggcacc ttatcacagc
      601 cgcttgacgc agttaggcgg acatttcttc cagagtgtgg gctgggagcg ccctcaatgg
      661 tacgagagca accgcaggct attggaaaaa taccgccaac aaatcccgga aagggaaggc
      721 tgggaagcaa tgtattggtc gccattgcaa ggggttgaac acctggcaac ccgtgagaat
      781 gtgggtttgt ttgaacttag ctccttcttt aagattgaag tctctgggcc aggtgcagca
      841 gattatttgg aatacctcac agccaatcag gttgccaagg atttgggcaa agtggtttat
      901 acatccttgc tgacagaaag tggaggcata aaaagtgacc tgaccgtaac ccgactggca
      961 gaggatcgtt tctggctgtt gactggcggc ggcagcggga tgattgatct ggactgggtc
     1021 aaccagcacg cccccaaaga tgggagtgtc catattgcag atatttcttc caggtacacg
     1081 gctatcgggc tgtgggggcc caaagctcgc caggtgctgg agtctgttgc ggaacaggat
     1141 gtctcaaatc atgcttttcc ttatttcact gctcaagagc tgatgctcga ggctatccct
     1201 tccctggcat ttcgtctttc gtacgttgga gaattgggct gggaaattta tacccccacc
     1261 gagtttggac aacgtttatg ggatattctc tggaatgcgg gacaagagag ccagatgatt
     1321 gctgcaggaa tgggagcgtt cgactcacta cggttggaaa aaggataccg cgccttgggt
     1381 aatgagctcc atgttgaata caatccactc gaagccgggc tgggttgggc tgtcaacctg
     1441 gaccagggaa attttctcgg gcgagaagcc tttctaaaga taaaacaatc tggcgttgat
     1501 aaaatgctat gctgcctgac attagatgaa ggcacagcct tagggaagga accaattttc
     1561 actggcgaca aatgtatcgg ctatgtcacc agtgccaact atggctacag tgtcggaaag
     1621 catatcgcct acggctacct gcctgctcaa tatgcaagaa agggtaccca ggtagagatc
     1681 gagtattttg gggagcgatt caccgcccac gtcgacaatg acccactata tgacccaggc
     1741 atgaagaagt taaaagccta agcaaggaga aatgaatggc aaagtacaaa gtgctttatt
     1801 ggtatgatat tccgactcag gtccggtcag aggatgaaaa cggccgcgcc agcctgcagc
     1861 tatctgaccg atttcaactc gctatcgacg aggcggctat ggcagcaaaa ttgatcagta
     1921 ctgatgacta tactgctggt tttatttggc accccgaaca ggaaaaagaa ggcagcgcaa
     1981 aagacgttgc ggctgtcgta gtcgctgaaa ttg
//
LOCUS       DBNGSX010000571         3291 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_127944, whole genome shotgun sequence.
ACCESSION   DBNGSX010000571 DBNGSX010000000
VERSION     DBNGSX010000571.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_127944"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="AC16CB_16675"
     CDS             complement(<1..622)
                     /locus_tag="AC16CB_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015113683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344159.1"
                     /translation="MNENNTISEDETFASLVFNGIDGASGGYLLPEMTPAQVSAIAQG
                     QTIEPENINELKNRAEQGPHYGFEGDARNLAESGWGVIFAHKAEPALMEALDPLLKHR
                     QEQAGERFKVYQGADGYRPGESKAAFLERHGKGPGSVSPEVVPYYLLIVGDPSTIPYR
                     VQYQLDVQYAVGRLAFDSIEDYARYAQSVVEAERDLKLPPRATFFGV"
     gene            complement(634..>3291)
                     /locus_tag="AC16CB_16680"
     CDS             complement(634..>3291)
                     /locus_tag="AC16CB_16680"
                     /inference="COORDINATES: protein motif:HMM:NF012618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344160.1"
                     /translation="REAAERERRLKLEAAEKVAEAERRRAEEQALAAKKLRTRFILAM
                     LAFIAALFFAGAAYYSGQQAMVARNFAYRESTRAVGNAATAEANAAIAATNAAIAATN
                     EFKAVAAEATANSEREIAEKARSTAVAAEKDALNQKATAEYNAEIAQQQANLARSREL
                     SNLSLSFIQDQPNLALLLSIEAYRKSDTGQALDALLSTLQRNLNREIQRSNVQIETQP
                     ISIDAMAISPDGHQLAWAGVQGLIRVYDFKLQKQIWQDTIGIKINQLVFSPDGKILAS
                     ADDTGEIIFWNPADGKKVRVIRPDIASIYAMDFSPDGTSLAYGGISQSNKVNLFALNL
                     NNLAIKNFSVRPLKTDILSLAWSPDGRLLASAGRERVVRIWDVATEKELVTFEIYEGP
                     MKSVVFSPNGQWLATGGNDEILPFDKNILLWDISLCDRPTDSANQGEQISPWDDPTCR
                     LQLPVSFSGQEADVTSLAFSPDGLVLASGDISGNTRLWDVVYRKPIDLQIPRARGIIS
                     QLAFSQIEDNLVLAVGSLDRTISLNNLRNTDSLMTENTTTQSAIGGMAFRADGTFQVI
                     GSDGAQTVLQTENEAGEVNQTGTFAPTSAVYALSKDTGSFAVLVEDNQEGRIEIWSED
                     DDSGPLLTIPSPAGDVITFSQAPNGQAISETLSTSVGTIRSLALSPDGATLAAGVCAV
                     SEKFIDPRLQASRTQAAGTERDVCTRNEIHLWQTGSGNLITQIPTDHRNAILSLAFSA
                     DGRILASGGADWTIYLRDIQEGRPIGLPLTGQAGSVTALAFSPDGSLLASGSSRSLVA
                     LWNVASAQLIGDPIAGMSSIVTALAFSPDNKTLVSGSDEAEVAWWRLDAWLDLACKYS
                     QRNLSQQEWEQFFKGEPYRQTCPEYPVGE"
BASE COUNT          632 a          892 c          965 g          802 t
ORIGIN      
        1 atacgccgaa gaaggtcgcc ctgggcggta gcttaagatc gcgctcggcc tctaccacac
       61 tttgggcgta gcgggcgtag tcttcaattg aatcgaaggc cagccgtccc acggcgtact
      121 gcacatcaag ctggtactgg acgcggtagg ggatggtgga cgggtctccc acaatcagca
      181 gataataggg aactacttcc ggggaaaccg agccaggacc cttgccatgc cgttccaaaa
      241 aggcagcctt ggattcgccc gggcggtagc catcagcgcc ttgatacact ttgaagcgct
      301 ccccggcctg ctcctggcgg tgtttgagca aagggtcgag ggcttccata agagcaggtt
      361 cagccttgtg ggcgaaaatc accccccagc cggattcggc tagattgcgc gcatcacctt
      421 caaatccata atgtggtcct tgttcagctc gattttttag ctcattaatg ttctccggct
      481 caattgtctg gccttgggcg atagctgaga cttgagcagg ggtcatctcc ggtaagagat
      541 agccgccgct ggcgccgtca ataccgttaa aaaccaggct agcgaaggtt tcgtcctcag
      601 agatggtatt gttttcgttc acagttgctc cttctactct cctaccggat attcggggca
      661 agtttgccgg tagggctcgc ctttaaagaa ctgttcccac tcttgctggc tcaagttgcg
      721 ctgggaatat ttgcatgcca ggtcaagcca tgcgtccaat cgccaccagg ccacctcggc
      781 ttcgtcgctg ccggagacca gggttttgtt atctgggctg aaggccagcg cagtgacgat
      841 gctgctcatg ccggcaatcg gatcgccgat caactgggct gaagcgacat tccacagcgc
      901 caccaggctc cttgagctgc ccgaagccag tagagagcca tctgggctga aagccagggc
      961 ggtgaccgag ccagcttgac cggtgagggg caggccaatt ggtcttccct cttggatgtc
     1021 tcgcaagtag atcgtccaat ccgcaccgcc gctggccagg atgcgcccat cggcgctaaa
     1081 agccaggctg aggatggcgt tcctgtgatc ggtggggatt tgcgtgatca aattgcccga
     1141 gccggtctgc cagagatgga tctcattgcg ggtgcacacg tcgcgctcag tgcccgcagc
     1201 ctgtgtgcga gaggcttgca ggcgcgggtc gatgaatttt tcgcttacag cgcagacccc
     1261 ggctgccagg gtggcgccat ccgggctgag ggccaggctg cggattgtcc cgaccgaggt
     1321 gctcaaggtc tcgctgatgg cttgaccatt tggggcctgg ctgaaggtaa tcacgtctcc
     1381 agcgggagat gggattgtca gcaatgggcc gctgtcgtca tcttcgctcc agatttcgat
     1441 gcggccttcc tggttatctt ctactaaaac tgcgaatgaa ccagtatcct tgctcagagc
     1501 atagaccgcg gatgttggcg caaaggtgcc tgtttggttg acttctccag cctcgttttc
     1561 tgtctggagc accgtttgtg cgccatcgct gccgatgacc tggaaagtgc catccgctct
     1621 aaaggccatg cctccaatag cgctttgggt ggttgtgttt tcagtcatca aggagtcagt
     1681 gttgcgcagg ttgttcaggc tgatggtgcg gtcgagactg cccacggcta gcaccaggtt
     1741 atcttctatc tggctgaagg ctagctggct gataataccc ctcgcccttg gaatttgcag
     1801 gtcgattggc tttcggtaga cgacatccca taagcgggtg ttgccggaga tatcgccaga
     1861 tgccagcacc aggccgtctg ggctgaaggc aaggctggtg acatccgctt cctggccact
     1921 aaatgatacc gggagttgca ggcggcaagt cgggtcatcc caagggctga tttgttcacc
     1981 ttgattggcg ctatctgtag gccggtcgca tagcgagata tcccacaaca ggatattctt
     2041 gtcaaatggg aggatctcat cattacctcc ggtagccagc cactggccgt tgggcgagaa
     2101 aaccacgctc ttcatcggcc cttcgtatat ctcaaatgtg accagctcct tctcggtggc
     2161 tacgtcccaa atgcgcacaa cgcgttcgcg cccggcagag gcgagcagcc ttccatccgg
     2221 cgaccaggcg aggctcaaga tatcggtctt gagagggcgc acgctgaaat tcttgatggc
     2281 gaggttattt aagttcaggg caaacaggtt gactttgttt gactgggaga tgcctccata
     2341 agccaggctg gttccatctg ggctgaagtc catggcatag atcgaggcaa tgtctggacg
     2401 gatgactctc actttctttc catccgctgg attccaaaat atgatctcac ctgtgtcatc
     2461 ggcggaggcc aggattttac catcagggct gaataccaac tggttgatct tgatcccaat
     2521 ggtgtcttgc cagatttgtt tctgtagttt gaaatcatac acgcggatca aaccctgcac
     2581 gcccgcccag gctagttggt gcccatcagg gctgatagcc atggcatcga tcgagatagg
     2641 ctgtgtctca atttgtacat tggatcgttg gatttccctg ttcaggttgc gctgaagcgt
     2701 gctgagcaag gcgtccagag cttgcccggt atccgatttg cggtatgcct cgatgctcag
     2761 caataatgct agattcggct ggtcttgaat gaagctgagc gaaaggttgg aaagctcacg
     2821 ggagcgagcc aggttagcct gttgctgtgc gatttcggcg ttgtactcgg cagtcgcttt
     2881 ttgattcagt gcgtccttct cggcggcaac tgcggttgat ctggcctttt cggcgatctc
     2941 ccgctccgag ttggcggttg cctcagcagc tacggcttta aattcgttgg ttgcagcgat
     3001 ggcagcgttg gttgcggcga tggcagcgtt ggcttcggcg gttgcagcgt ttccgactgc
     3061 ccgcgtgctt tctctataag caaaatttct ggcgaccatt gcctgttggc cactgtaata
     3121 ggccgcgcct gcgaagaata gggcggcaat gaaggccagc atggctaaaa taaagcgcgt
     3181 gcgtaatttt ttggcggcca aggcttgttc ttcagcgcgg cgtcgctctg cctctgccac
     3241 cttttcggct gcctccagct tgaggcgtcg ctcgcgctcg gcagcttctc g
//
LOCUS       DBNGSX010000572         4702 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1236188, whole genome shotgun sequence.
ACCESSION   DBNGSX010000572 DBNGSX010000000
VERSION     DBNGSX010000572.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1236188"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..624
                     /locus_tag="AC16CB_16685"
     CDS             286..624
                     /locus_tag="AC16CB_16685"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3344161.1"
                     /translation="MASPLEKEVNQLHAEICAGLADPKRILILYALADKPCNVNELAN
                     YLKLSQPMVSRHLKVLRERGMVQANRVGVSVEYKLGDIRLIQALDLLRAVLHDDLSHR
                     AELVGALGEE"
     gene            665..1198
                     /locus_tag="AC16CB_16690"
     CDS             665..1198
                     /locus_tag="AC16CB_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003389587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HIR3344162.1"
                     /translation="MTVVDLPLANTLTVQSNEKSTKNKLSMVIFSGDMDKILAAFIIA
                     TGAAASGMEVTMFFTFWGLKAIQKSRVITGKGLFGRMLGMMNRGGLDAIGPSRLNMGG
                     MGRWMFKLMMKQKGVASLPELYEMAVDLGVKMMPCQMSMDVMEIDPQLFQDNVEPPVG
                     VAAFIEEAAESKATLFI"
     gene            1224..1463
                     /locus_tag="AC16CB_16695"
     CDS             1224..1463
                     /locus_tag="AC16CB_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR3344163.1"
                     /translation="MSTQTTGMVLDLKGLLCPMPVIKLAKAIKDIPVGEIIEAFATDP
                     GVMADIPAWCRTTGNELVTLEKNDKQFRFVVRRVK"
     gene            1474..2112
                     /locus_tag="AC16CB_16700"
     CDS             1474..2112
                     /locus_tag="AC16CB_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344164.1"
                     /translation="MSFSPFSQILVFLALIFTVFAFGRKLKIYAQLARPVDRAKPKGD
                     LQKGVLYAYTLGMAPWSKESTRLHALAYLRGVAFHVGIFLGLAILVASPWLGEMPQYV
                     RNILGFVIAAGALLGLLGFVARFLEHNLKALSNADDYFAVMIVGLFLAAEALWLFFPA
                     ARVLFYLVSAAMLVYAPLGKIRHCIYFAYSRLFYGRFIGSRAVLPHSQQNAR"
     gene            2116..3525
                     /locus_tag="AC16CB_16705"
     CDS             2116..3525
                     /locus_tag="AC16CB_16705"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR3344165.1"
                     /translation="MTTTKIDIALNLLNQQLNQPLTAALEVCARCGICAEACHYYVAE
                     PKVEHIPAARAESLRKVYRSEHDLLSRLFPSWTGAERLTEEKLAEWADIAFNKCTLCY
                     RCTVNCPMGLDTPLLMTSLRAIATATGNAPEVLEMLADAAIEKGKDPEIYLGFYKESI
                     KDMEVELQELTGNPDASIPLQKQGANKLYVALAGAHTILPVAAIFETVGEDWTLSIFE
                     AANYGIFLGDASRAKAIARRIVDEAKSLGVEEIVISECGHAYYVMRWVAPNWFGDEFT
                     FRVRSIVEVLAEYVQDGKLKLDPSANSEPITYHDSCNLGRKGGILAEPRVVLQAVTND
                     FRELTPNREEAYCCGGGAGLVALPEAYDLRMMAGKPKADQVKRSGAQVVVAACENCRL
                     QLGDLSGYYGLGIGVTALTDLVVKAMRLPGNQKTVEHIMTLETANSGEHDNAEVWGST
                     DLVSLPSTKGDRSEPKSNV"
     gene            3830..>4702
                     /locus_tag="AC16CB_16710"
     CDS             3830..>4702
                     /locus_tag="AC16CB_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HIR3344166.1"
                     /translation="MGIAILLELGHGTDERLRHSLRPLAAFGLLHGANEWIEMFDKLG
                     LLFGFPETQIAWEALRLMILAFSFLSLGAFGSSLLAENERSRRLSLLLPLSLAAIWGL
                     GLYSMRGKFSFVDIVDVTDVWTRYVLAVPSALFACAGLIVQQRRFRQAGMAQFGRDSL
                     WAAIAFFWYGIIGQTFTRASPLPPSTIINQGLFLEVFGFPVQLLRAGAAVLAAVFVMR
                     FLRSFEVEMHNQLAALHEAQLQEAERREALRGDLLRRVVEAQEAERQRIARELHDETG
                     QALTALGLGLRGASA"
BASE COUNT         1171 a         1020 c         1216 g         1295 t
ORIGIN      
        1 aatatgcaac ctttaccttt cggattttgg ttaaagagcc aaagtccagc catgccacgt
       61 atccaactgc gtaacttcta ttaatattaa cgacacatca atttgtcaaa aaggatttac
      121 gatggtcggc atgatcatag agatgcgcta acaaggttta gtgataaata tcacaagttg
      181 ttgtgatatt tatcactatg gagaatttat tcaaatagtt agaattgcat atgtatttat
      241 atatgcttat ccatatattt acgtatgata aaggaaaagt attatatggc atctccacta
      301 gaaaaagaag taaatcaact ccatgctgaa atttgtgcgg gattggcaga ccctaaacgt
      361 atattgattt tgtacgccct cgctgataag ccctgcaatg tgaatgagtt ggcaaactac
      421 ctgaagctga gccagccaat ggtctcccgt cacctcaaag ttctgcgcga gcgcgggatg
      481 gtacaagcca atcgagtagg tgtatctgtg gaatataagc tgggcgatat tcgcctgatc
      541 caagctctgg acctgctccg tgcggtcctc catgatgacc tatctcatcg ggctgaatta
      601 gttggtgcgc ttggtgaaga ataaactcaa ttaaacctgg tatatcaaag gagttacctc
      661 acccatgaca gtcgtagatc tccctctcgc taacacgtta acagtccaat cgaatgaaaa
      721 aagcaccaag aacaagctat ctatggtgat ttttagcggc gatatggata aaatactcgc
      781 tgcgttcatt atcgccaccg gtgcggccgc ctcaggtatg gaagtcacaa tgttctttac
      841 attctgggga ttgaaggcga tccaaaaatc tagggtaatc actgggaaag gtctttttgg
      901 acgaatgctc ggaatgatga accggggtgg cctggacgct attggcccaa gccgcctcaa
      961 tatggggggg atgggacgct ggatgttcaa gttaatgatg aagcaaaaag gtgttgcctc
     1021 tttacccgag ctgtatgaaa tggctgtaga tttaggggtc aaaatgatgc catgccaaat
     1081 gagcatggat gtgatggaga ttgacccaca actattccaa gacaatgttg aaccgcccgt
     1141 gggtgtggcg gctttcatag aagaggccgc tgaaagcaaa gcaaccctct ttatctaaaa
     1201 attcaaatta ggagaatatt taaatgtcta cacaaaccac tggtatggta ttggatctta
     1261 aaggtctact gtgccctatg cccgttatta aacttgcaaa ggcgatcaaa gatattcctg
     1321 taggggaaat aattgaagca ttcgcaacag atccgggtgt tatggcagac atacccgctt
     1381 ggtgccgcac aacggggaat gaattagtca ctctggaaaa aaatgataaa caatttcgtt
     1441 ttgttgttcg acgggtgaaa tgaggcctag caaatgtcgt tttctccatt tagtcaaatc
     1501 cttgtttttc tggcgctcat ttttaccgta ttcgcatttg gtcgaaaact gaaaatttat
     1561 gctcaactcg cgcgaccagt cgatcgggca aagcctaaag gtgatctaca aaagggtgtc
     1621 ctttatgcct acaccttggg tatggcgccg tggtcgaagg aaagcaccag gcttcacgct
     1681 ctagcatacc tgcgtggtgt ggccttccac gttgggattt ttcttggtct ggcaatattg
     1741 gttgccagcc cttggctcgg tgaaatgccc cagtatgttc ggaacattct agggtttgtc
     1801 attgctgctg gagctctatt aggtttattg ggttttgtcg ctcgcttttt ggaacacaat
     1861 ctgaaagcgc tgagtaatgc agatgattat ttcgcagtaa tgattgtcgg tctattcctg
     1921 gcggctgaag ccctatggtt gttcttccca gccgcgcgag tgctttttta tctggtgagc
     1981 gcggcgatgt tggtatatgc cccattaggt aaaatccgcc actgtatcta ttttgcatat
     2041 tcgcgtctgt tttacggtag atttatcgga agccgagcag tgctgcctca tagtcaacag
     2101 aatgcaaggt gagccatgac gactacaaaa atcgacatcg ccctgaacct gcttaatcag
     2161 caactcaatc aaccgcttac agctgcattg gaagtctgtg ctcgctgtgg aatctgcgct
     2221 gaagcctgtc actattatgt agcagaacca aaggtagagc atatccctgc tgcacgtgcc
     2281 gagtcgctgc gcaaggtata ccgcagtgag cacgacttgc tcagtcgcct ttttccttcc
     2341 tggactggcg cagaacgact gactgaggaa aagttagctg aatgggctga tattgccttc
     2401 aacaaatgca cattgtgcta tcgctgcaca gtcaactgcc cgatggggct agatacaccg
     2461 cttttgatga ccagcttgcg agcaattgcc actgccaccg gtaacgcccc cgaagtgcta
     2521 gagatgcttg ccgatgctgc tatcgagaag ggtaaagatc ctgagatcta tctgggcttt
     2581 tataaggaaa gcatcaaaga catggaggtc gagcttcagg agctcaccgg taaccctgat
     2641 gcctcaattc cccttcaaaa acaaggcgcg aataaattat atgtcgcttt ggctggtgca
     2701 cataccatct tgcccgtggc tgccatattc gaaacagtgg gtgaagactg gacgttgagt
     2761 atatttgaag ctgctaatta cggcatcttt ttaggtgatg cttccagggc aaaagcgatc
     2821 gcacggcgaa tcgtggatga agccaaatct ttgggtgtgg aggagattgt aatctctgaa
     2881 tgtggtcacg cctattatgt gatgcgctgg gttgcgccta actggtttgg cgatgagttc
     2941 acttttcgcg tgcgcagcat tgtggaagtg ctggcagaat acgtgcaaga cggcaagctg
     3001 aagctggacc ccagtgcaaa ttcagagccg atcacctatc atgattcatg caacctgggg
     3061 cgcaaaggtg gtatcctggc tgagccgcgc gttgttctac aagctgtgac aaacgatttt
     3121 cgagagctaa cgcctaaccg ggaggaagcc tattgctgcg gtggcggcgc tggtctggta
     3181 gcacttccag aggcttatga tctgcgtatg atggcaggta agcccaaagc cgatcaggtg
     3241 aagcgctcag gcgctcaggt tgtagtagct gcgtgcgaaa actgccgcct tcaattgggt
     3301 gatttgagcg gttattatgg cctagggata ggggtgacag ccttgactga tctggtcgtc
     3361 aaagcgatgc gcctgcccgg gaatcaaaaa acggtggaac atatcatgac acttgagacc
     3421 gccaattcag gagagcatga taatgctgaa gtgtggggat ccacagattt ggtttcactc
     3481 ccttccacga aaggggatcg ttccgaacca aagtcgaatg tgtaaagaga agtatgcatg
     3541 aacattcgcg ttcaggactt tccctgagat actttttcaa gttagatcat gggcgtacga
     3601 agcgtaggag aatattttaa taaggtcgca agaatagtgt gcctgctctt gcgacctttt
     3661 ttcgatagca tgatacattt gtgtgttagc agggaaaaac ccaatgcagg ttattttggg
     3721 tgacttgtta taattaagat cagaaacttc atccggtaat attggggtgg ttgtgtctcc
     3781 ttcttatatt gcattcattt tcttttatta cgggctagca ttcttcagta tgggtatagc
     3841 catattgtta gagttggggc atggcactga tgagagactg aggcactctc ttagaccgct
     3901 ggcagccttc ggactccttc atggtgccaa tgaatggata gagatgttcg ataaacttgg
     3961 tctactgttt ggctttccgg aaacacagat cgcttgggaa gctttgcgcc tcatgatact
     4021 ggcgttctcg tttctttccc taggggcatt cggttcttcc ctcttagctg agaatgaacg
     4081 aagccgtcgt ctgagtttgc tcttgccgtt atcattggct gcaatttggg ggcttggtct
     4141 ttacagtatg cggggaaaat tctcttttgt agatattgta gatgtcaccg acgtgtggac
     4201 acgctatgtt ttggctgtgc catctgcttt attcgcctgt gctggcttga tcgtgcaaca
     4261 acgccggttt cggcaggcgg gtatggctca atttggaaga gacagcctgt gggcagccat
     4321 tgcttttttt tggtatggga tcatcggaca aacattcact cgagctagcc ctttgcctcc
     4381 ctctacaata ataaatcaag gtctatttct agaagtgttt ggctttccag ttcaattgct
     4441 gcgcgctgga gcagcggtat tggcagcggt atttgtgatg cgttttttac gctcatttga
     4501 ggtcgagatg cacaatcagc tcgccgcgct gcatgaagcg cagctacaag aggctgagcg
     4561 gcgtgaagca ttacgaggtg acttgctccg ccgggttgta gaagcccagg aagcagaacg
     4621 gcagcgtata gctcgcgagc ttcacgatga aacgggccag gctctgacgg ctttaggtct
     4681 tgggctgcgc ggtgctagcg ct
//
LOCUS       DBNGSX010000573         3680 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1651219, whole genome shotgun sequence.
ACCESSION   DBNGSX010000573 DBNGSX010000000
VERSION     DBNGSX010000573.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1651219"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="AC16CB_16715"
     CDS             complement(<1..1223)
                     /locus_tag="AC16CB_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012407375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3344167.1"
                     /translation="MAKPVILAIDDDPMVLSAVDRDLRKKYGQDYRIVPINMGSAAMQ
                     YLKQLQGRAEVVALFLVDQRMPQMTGVEFLEQARHVYPEAKKVLLTAYADTEAAIKSI
                     NKVSLDYYLMKPWDPPEERLYPVLDELLGEWKAHVRMPYEGIRLAGTLWSSASHNTRD
                     FLTRNLIPYQWLDVEKDAKVRALVEEYNQGELRIPTLFFPDGSVMVEPNLRELAEKVG
                     LKTRASLPYYDLIIIGSGPAGLSAAVYASSEGLSTLAIERGTPGGQAGSSPKIDNYLG
                     FPNGISGNELARRAMTQARRLGAEILTAQEVTHISLQDPYRIVRLSDNTEISCRAVLL
                     ATGASFHMLRMPGAAALSGAGVYYGAAYTEARSYKDQDVFVVGGANSAAQGALYLSRF
                     AHKVNLIVRGELSAA"
     gene            complement(1290..1643)
                     /locus_tag="AC16CB_16720"
     CDS             complement(1290..1643)
                     /locus_tag="AC16CB_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344168.1"
                     /translation="MGRLADILKMLLVYGRRVALIVGMVMTIITFYGSGAVDITARFY
                     FRSGIQYLVYLSIIVGILTFIKLMEFFGLCDELTLSFLDDDGRFGRALAARHQLGCDI
                     VIPFNIRLFAPIVVG"
     gene            complement(1769..>3680)
                     /locus_tag="AC16CB_16725"
     CDS             complement(1769..>3680)
                     /locus_tag="AC16CB_16725"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR3344169.1"
                     /translation="GKIRHPFVPPPSMYDMAYFFGGEDTMEEYGIPRGQFTRIMFRVT
                     PYTDDHAREVASVVKERLAKQGVSVAATMYQDPNKHWGRAFVDGMALVTKVLAVISML
                     LSAVLVSNTLMAIITQQTNQLGILKAIGGTSFTVLRVYLSGVVIYGLLSLGIALPLGS
                     LASFRISSWFLSLYNIEYTQFTSSPQATIFMVVAALVVPLAAALIPILQGAAITVRQA
                     IASYGLGGDFGSNWIDRLVEGIGRRFLISYQAMALANTFRRKGRLLLTQLVLIIAGVM
                     FLMVMSLSSSIDATLDAEFARRDYDVVISFDDLQRVDRTVAMMEATPGVDHAGMWLAV
                     PATILHQGQKMLDAGLGSQLQGVPVEDPMYKPLIVEGRWLQPGDRRVIVMNKTTADDE
                     DIRVGDTVKLDLGVMGESDWQVVGLYRVFLMFGGGFNIDALYGPRDVVFAATKKTGRA
                     NLLLVRTQNHAPEAVELTASQLGDLFREHNIEISQTETMPSTRRTSDVSFSYVVIMLL
                     VLAVIVAMVGGIGLMGALWISVIERTKEIGVMRAIGALSRTIMGMFILEGIVQGLMSW
                     VIAVPLSLLVTPIMANALGMAMFNSRLDFQFNIQAVFIWLGTILVVALVAAIIPARNA
                     TRINVRQSLNYE"
BASE COUNT          923 a         1107 c          856 g          794 t
ORIGIN      
        1 tgggcagcgc tgagctcacc ccgcacaatt aagttcacct tgtgcgcaaa acggctcagg
       61 tacaatgccc cttgtgccgc cgaattcgcc ccaccaacaa caaacacatc ctggtcctta
      121 tacgagcgtg cctcggtata ggcagcacca taatagaccc ctgccccaga cagggcagct
      181 gcgcctggca ttctcaacat atgaaaagaa gctcccgttg ccagcaacac agcccggcag
      241 gagatttcgg tgttatcgga tagccgcacg atgcgataag ggtcctgcag gctgatatgg
      301 gtaacttcct gagcggtcaa tatctccgcc cccaaacggc gggcttgcgt catggcgcgc
      361 cgcgccagct cattgcccga gataccattc gggaaaccaa ggtaattgtc gatctttggg
      421 ctgctcccag cctgaccgcc cggagtgcct ctttctattg ccaaagtgct caatccctcc
      481 gagctggcat ataccgccgc cgacaaccct gccggcccgc tgcctatgat gatcaaatcg
      541 tagtatggca atgacgctcg ggtcttcaag ccaacttttt cagccagttc acgcaggttt
      601 ggctcaacca tcaccgaacc atccgggaag aacagggtgg gtattctcaa ttctccctgg
      661 ttgtattctt cgaccaaggc gcggaccttg gcatcctttt caacgtccaa ccactggtat
      721 gggatcaagt tgcgtgtcaa gaaatccctg gtattgtgcg aagcagatga ccacaaagtg
      781 cccgccaggc ggataccctc atatggcatc ctgacatgcg ccttccactc cccaagcaat
      841 tcgtcaagca ccggatatag acgctcctcg ggtggatccc atggtttcat caggtagtaa
      901 tccaggctga ctttattgat agatttaatc gccgcctcgg tatccgcata ggcagtcaac
      961 aacactttct ttgcttccgg gtacacatgg cgcgcctgct caagaaattc cacgccagtc
     1021 atctgcggca tgcgttgatc taccaggaac agggcaacga cctcggctcg cccttgaagt
     1081 tgcttcagat attgcattgc ggcactgccc atattgattg ggacgattcg atagtcctga
     1141 ccatatttct ttcgcaggtc gcggtccacg gcgctcagca ccatcggatc atcatcaatt
     1201 gcaaggatta ctggttttgc catctcgcct ctttctttga ttatattcaa gtgcgcggcg
     1261 ccagccgcac ttaaaataaa tcggattgtc tatccgacca ctattggcgc gaataagcgt
     1321 atgttgaacg gtattactat atcacaacca agttgatgcc tagcagcaag cgctctccca
     1381 aagcgcccat catcgtcaag gaaagataac gtcaactcgt cacacaatcc aaaaaattcc
     1441 atcagcttga tgaatgtcaa tataccgact atgatggata gatacactaa atactgaatt
     1501 ccagaacgaa aataaaaacg agcggtgata tcgaccgcac ccgacccgta gaaagtgatg
     1561 atggtcataa ccatccctac aattaaagcc accctgcgac catacaccag aagcatcttc
     1621 agaatgtctg ctaatcgccc catccttgct caaacagctc cctattgtga ggatatgctt
     1681 gccacccgac aattcacaac gaatatcata ccatcctcag ggactgtata caagactatg
     1741 tgtataataa ttctctaata tacccttcct actcataatt caggctttgc cgcacgttga
     1801 tgcgcgtcgc attgcgggca gggataatgg cggctaccaa tgccaccacc agtatcgtgc
     1861 ccaaccagat gaataccgcc tgaatgttaa attgaaaatc tagacgagaa ttaaacatcg
     1921 ccatgcccag agcgtttgcc atgataggtg tgaccagcag cgagagcggc acggcaataa
     1981 cccagctcat caatccctgc actatgcctt ccaatatgaa catacccata atcgttcgcg
     2041 agagcgcccc gatggctcgc atcaccccga tttctttggt gcgctcgatc acgctgatcc
     2101 atagcgcccc catcagcccg atgcccccca ccattgccac aatgaccgcc agcaccagca
     2161 gcatgatgac tacatacgaa aacgatacat cagacgttct gcgcgttgat ggcatggttt
     2221 ccgtctgtga aatctcgata ttatgctccc tgaaaagatc gccaagctgg ctggcggtca
     2281 gctctactgc ctcaggtgcg tggttttggg tgcgcaccaa caaaagatta gccctgccgg
     2341 tttttttggt agcggcaaag accacatctc gcggcccata gagcgcatcg atattgaagc
     2401 ccccgccgaa catcagaaac acccggtata aacccactac ctgccagtcg ctttctccca
     2461 tgacgcccag gtccagtttc actgtatcgc ccacccggat atcctcatcg tccgcggtgg
     2521 tcttattcat cacgatcacc cgtctatcgc ccggttgcag ccagcgaccc tccacaatca
     2581 atggcttgta catgggatct tcgacgggca ccccttgcaa ttgcgagccg aggccggcat
     2641 ccagcatttt ctgcccttga tgcaatatgg tggcaggcac tgccagccac ataccggcat
     2701 gatctacccc aggtgttgcc tccatcatag caaccgttcg atcaacacgt tgcaaatcat
     2761 caaaggagat gaccacatca taatcgcggc gcgcgaattc agcgtccagc gtggcgtcga
     2821 tcgagcttga aagactcatc accatcaaaa acataacccc ggcgatgatc agtaccaact
     2881 gggtgagcag caatcgtcct tttcgccgga atgtattggc tagagccata gcttgatacg
     2941 agatcaagaa gcgccgccca atgccctcca ccagccggtc aatccaattc gagccaaaat
     3001 ccccacccag gccatagctg gcgatagcct gcctgaccgt gatggccgcg ccctgcagga
     3061 tagggatcag ggcagcggcc aaaggcacta ccagtgcggc caccaccatg aaaatagtcg
     3121 cttgcggcga cgatgtaaat tgagtgtact cgatgttata caagctcagg aaccagctag
     3181 agattctaaa ggacgccagc gaccccaggg gcagggcgat gcccaacgag agcagcccat
     3241 agatcaccac cccgctcaga taaacacgca aaactgtaaa gctggtgccc ccaatcgcct
     3301 tgaggatgcc aagctggttg gtttgctggg tgatgatcgc catcagcgta ttggaaacca
     3361 gcactgcgct gagcaacatg gagatcaccg ccagcacttt tgtgaccagc gccatgccat
     3421 ccacgaaggc acgtccccaa tgcttattcg gatcctggta catggtagcc gcgacgctaa
     3481 ccccctgctt ggccaggcgc tccttgacca ccgaggccac ctcacgggcg tgatcgtcag
     3541 tataaggagt cacgcggaac ataatgcggg taaactggcc gcgcgggatg ccgtactcct
     3601 ccatggtatc ttcaccaccg aaaaaatagg ccatatcgta catgctgggc ggcggcacaa
     3661 agggatggcg aatcttgccg
//
LOCUS       DBNGSX010000574         2146 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1929761, whole genome shotgun sequence.
ACCESSION   DBNGSX010000574 DBNGSX010000000
VERSION     DBNGSX010000574.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1929761"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="AC16CB_16730"
     CDS             complement(<1..595)
                     /locus_tag="AC16CB_16730"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014215.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3344170.1"
                     /translation="MLEEQLDRGANYSRYRASYLSEGFKIYALLTIPDGEIPPTGWPA
                     IVFNHGYIPPAQYRTTERYIAYVDWLARSGYIVFRIDYRGHDRSEGEARGAYGDTGYT
                     IEVLNAVASLKRFPQADPDRIGMWGHSMGGYLTLRAMVISPDIKAGVIWAGVVGSYPD
                     LLNRWRRRGGTIPTQAVTSSRRWRVSWLEQFGSPEENP"
     gene            complement(964..1680)
                     /locus_tag="AC16CB_16735"
     CDS             complement(964..1680)
                     /locus_tag="AC16CB_16735"
                     /inference="COORDINATES: protein motif:HMM:NF019413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HIR3344171.1"
                     /translation="MMPDSGQTFPKLPLVIIACQVFQDLIRRLITDEMEAQIVFLDYG
                     LHQVPKKLTQALQDTIDRIDQPSLVVLGYGLCGNGLHGIKAGKHFLLVPRADDCIAIL
                     LGSYSAYRKEFDSSPGTYYLSKGWLESGSNPLSEYKEYEARLGSEKAMWVMDMQYQNY
                     KRLVMVAHTPEDLEKYRPMALDVARFCKRWGMRYEEILGSDSFVRKLIEVASLPDQAN
                     EEFVVIPPGGELKQSAFIRW"
     gene            complement(1841..>2146)
                     /locus_tag="AC16CB_16740"
     CDS             complement(1841..>2146)
                     /locus_tag="AC16CB_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018326277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HIR3344172.1"
                     /translation="RTRNLADVCRSADVLVAAVGRPEMVRGDWVKPGAVVIDVGVNRV
                     DDPSKEKGYKLVGDVAFDEVKEVASHLTPVPGGVGPMTIAMLLRNTVRAAQHADAKL"
BASE COUNT          549 a          567 c          491 g          539 t
ORIGIN      
        1 cagggttctc ttcgggtgag ccaaactgtt ccagccaact tactcgccac cttctagaac
       61 tggtaaccgc ctgggttggg atggttccac ccctcctgcg ccaacgatta agtaaatccg
      121 gataggaccc aactacacca gcccagataa cccctgcctt gatatccggg gagatcacca
      181 tagctctcaa tgtgagatac ccacccatcg agtgtcccca catgccaata cgatctggat
      241 cggcttgagg aaaccgcttt agggaagcaa cagcattaag cacctcaatc gtatagcctg
      301 tatccccata agcacctctt gcctctcctt ctgagcggtc atggccacga taatctatgc
      361 gaaatactat atatccactg cgtgcaagcc aatcgacata agctatatag cgctcagtag
      421 ttcggtattg cgctggcgga atatagccat gattgaagac aatcgcaggc cagcctgttg
      481 ggggtatttc accatctggt atggtcagca atgcatagat tttgaaacct tccgatagat
      541 aggatgctct gtagcggctg taatttgccc cacgatccag ctgctcttca agcacgatct
      601 cactcccagg gtaggacaat taacgcatcg ccaggatgtg catgggatgt agcgtcggcg
      661 tgggtgtata cgttgcagtc caggtagtcg ttggcgtggg agtgcttgta aacgtaggcg
      721 taagcgaagg gccaaatgtg gctgtagggg gcggtagact ggtagaaaca gcttggatag
      781 tactagaagg aaccgggctc ccggtattcc cagttgcaca cgcagcgggc agggccatta
      841 tccataataa aaatagcaaa aacggggttc ttttcatggg tatcaccaat aaaaaactac
      901 tcatcaaacc gtatttagat agagaaagaa gtagctttcc ctatattcat ccaactgcaa
      961 agcttaccag cgtataaaag cgctttgctt gagctcccct cctgggggga tgaccacaaa
     1021 ttcttcattc gcttgatctg gcaagctcgc cacctctatg agtttgcgga cgaagctatc
     1081 agatcccagg atttcttcat aacgcatccc ccaccgcttg caaaatcgtg ccacatccag
     1141 tgccatgggt cggtatttct ctaaatcctc tggtgtatgg gcaaccatta caagcctctt
     1201 gtaattttga tattgcatat ccatcaccca catggctttt tctgaaccaa gccgcgcttc
     1261 gtattctttg tattctgaca gaggattgct cccagattcc aaccagcctt tggagaggta
     1321 atacgtccca ggtgaagagt cgaattcctt gcgatatgcg ctgtatgaac ctaataaaat
     1381 ggcaatgcaa tcatcagccc gcggcacgag caggaaatgc ttacccgctt taatgccatg
     1441 caaaccgttt ccgcaaagcc cataaccaag aaccaccagg ctaggctggt cgatgcggtc
     1501 aatagtatcc tgtagggctt gagtcagctt tttgggtacc tggtgtaagc cataatccag
     1561 gaatacaatc tgagcttcca tctcgtcagt aattaatcgt ctaattagat cctgaaaaac
     1621 ctggcaggcg atgattacga gcggtaattt ggggaaagtc tgtcctgaat ccggcatcat
     1681 cactcctcat ccgttaccga taaactttac tctaaaattt taccttcctt ttgctgaaaa
     1741 acacaagata gggtcccaac gagtaattat caccctttgt gaccctatct ccgagctaat
     1801 ttgataccga aaaggctaag acaatttaga tattgaggtc ttacaactta gcgtctgcat
     1861 gctgtgcagc tcgcacagta ttgcgtaaca gcatggcaat ggtcatcggt cctaccccgc
     1921 cgggtactgg tgtgagatgc gaagcgactt ctttgacttc atcgaaggcc acatccccta
     1981 caagtttgta tcccttctct ttactggggt cgtcaactcg gttcacgcca acgtcgatca
     2041 ccactgctcc gggcttgacc caatcgccgc gcaccatctc tggtcgtccc acagcagcca
     2101 caagtacgtc tgcagagcgg catacatccg caaggttgcg tgtgcg
//
LOCUS       DBNGSX010000575         8013 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_613615, whole genome shotgun sequence.
ACCESSION   DBNGSX010000575 DBNGSX010000000
VERSION     DBNGSX010000575.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8013)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8013)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8013
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_613615"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="AC16CB_16745"
     CDS             <1..241
                     /locus_tag="AC16CB_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3344173.1"
                     /translation="KARQLTPLADELGCSMAQLALAWCLKNQHVSTVITGASRPDQVS
                     ENLGSLEVVGKLTPDIMERIEEILDNKPEPIEDER"
     gene            complement(743..1564)
                     /locus_tag="AC16CB_16750"
     CDS             complement(743..1564)
                     /locus_tag="AC16CB_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_095042061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HIR3344174.1"
                     /translation="MDTRIVAVYCLCDDMLKALHHRDDPQCQMTDAEVMTTAIVAALD
                     YGGNHEKARRMLQAEGYVPNMLGKSRFNRRWHRNADLFLTLFNLLGETWKQLNSNSIY
                     VIDSFPVAACDNYRIQRCQIYQEEDFRGYQASKRRFFYGLKIHLMITETGQPVEFFLT
                     PGSYSDTSALKLYDFDLPEGAKLTGDKAYNDYEVEDVINQAGISFIPLRKKNSKRPLP
                     PWVHYLRASYRKLIETTGSLIEQLLPKHIHAVTSRGFELKVALFVLACSINYLVG"
     gene            complement(1707..1916)
                     /locus_tag="AC16CB_16755"
     CDS             complement(1707..1916)
                     /locus_tag="AC16CB_16755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344175.1"
                     /translation="MSEPQNAPTPKSGSPVYTACKGIIFYEDDTPTAVFGERRLYFCL
                     PSCLRAFQEDPKTSCLADEINSENN"
     gene            complement(1913..2092)
                     /locus_tag="AC16CB_16760"
     CDS             complement(1913..2092)
                     /locus_tag="AC16CB_16760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344176.1"
                     /translation="MLFLPYLPDHPAETSGYMIAGYVVIFGVMLIYLASLILRSRSLS
                     QDLELLEDLQDESEA"
     gene            complement(2105..2803)
                     /locus_tag="AC16CB_16765"
     CDS             complement(2105..2803)
                     /locus_tag="AC16CB_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HIR3344177.1"
                     /translation="MGSKPTLLKILDVVTVILLVVATGMVFFYAPMEAVMGQVQRVFY
                     FHVAAGWVGMLSFLVAAIAGIVYLRSGRREWDIVGLSAVEIGMVFAFINIVTGSIWAR
                     PIWNTWWTWDPRLTTATIMELIYAAYLMLRQGIEDPDRRARFGAVYAIVGFLSVPLTF
                     FSARLFRTIHPVVIGTNQPGAEGAFDMTPLMLQTFLFSLLTFTFIFADLLWHRIRLGR
                     FAEKVEQLKLKAMI"
     gene            complement(2908..3636)
                     /locus_tag="AC16CB_16770"
     CDS             complement(2908..3636)
                     /locus_tag="AC16CB_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA];
                     GO_process: GO:1903607 - cytochrome c biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1904334 - heme import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HIR3344178.1"
                     /translation="MSDTPQVMAHKDPLEGRAAFSFLRAMGAVVWKDLAAELRSRELL
                     SSMLVFALLVILIFNFALELDARERANVTSGVLWVTFTFAGTLGLNRSMAIEKDRGCL
                     DGLLLAPVDRSAIYFGKTLGNLIFMLLVEIIVLPIYSVLYNTNLFLPGLLLVILLGSV
                     GYVAVGTLLASMAVQTRTRDILLPILLFPVVIPVVIASVKGSGGYLQGIPFDEIMPWI
                     NLLIVYDVIFTAVAFMVFDYVVEE"
     gene            complement(3633..4340)
                     /gene="ccmA"
                     /locus_tag="AC16CB_16775"
     CDS             complement(3633..4340)
                     /gene="ccmA"
                     /locus_tag="AC16CB_16775"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HIR3344179.1"
                     /translation="MIEVRRLVKRFGLKAVLRGLEFHVEPGEFVALLGPNGAGKTTFL
                     RILASLSRPTLGEVRIAGYRLPAQAAAVRRRLGVVSHLPLLYGDLTAEENLQFYGRMY
                     GINDLAMRIEEVLNLVGLWPRRRDLVRTFSRGMQQRLAIGRAVLHDPDVMLFDEPHTG
                     LDQDASSMLDNVLGEVAARGRTVVMTSHDLSRAADLASRFDVLSRGVITASVKREEID
                     TRDLLAFYRQAVNGGTA"
     gene            complement(4372..6327)
                     /locus_tag="AC16CB_16780"
     CDS             complement(4372..6327)
                     /locus_tag="AC16CB_16780"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3344180.1"
                     /translation="MHRQSQPRWLRQITSRFVLLPFALLLFSLIISACSFSLAADITP
                     PPGSEQRPPIAVTQPAPISGPLYPLVPPNLESGKAIFEEKCAPCHGDTGLGDGPQSAQ
                     LPNPVAAIGSIELARQSTPARWYTQVTQGNLQKFMPPFNSLSDRQRWDVVAYSFSLTT
                     PPELVDQGQELYQANCARCHGGTGKGDGQDAAGGPKPPTNLTDQAFMAEKSADDFFQV
                     TKNGLPPAMPAFGAQLSEDEIWALAAYLRSLTFTAPDEPVAVAETPTPGEAATPTSAS
                     ATPIAEVPAGIGTVTGKVISAAGVELPEEMEILLHGFDNMQVAMTQTTTTQTDGSFIF
                     PDIQMPDGRVFLATVNYGRTSYGSDIGIAQDGINILDLPIVVYETTSDASILKADRLH
                     LFFEFVDAKTVRVIELYIVSNPSDKSLVAPEPGEASVVFSLPDGATKLEFQDGALGGR
                     YIKTADGFGDTVSIPPGFGSYELLFAFEMPYDRNLELAHPVTMPVDAIVILAPEGDIK
                     IKSDILQDSGVRDVQGTQYRTYSGGGLAAGDELRLTLTGKPSIGAPSLTTSSNTSLLV
                     GLSVFGLALIMAGVWLYRRTRLNGADSLDAEQALDSSDSADFETSDSLMDAIIALDDL
                     YQAGQLPDEAYQQRRDELKRRLKELIG"
     gene            complement(6358..6882)
                     /locus_tag="AC16CB_16785"
     CDS             complement(6358..6882)
                     /locus_tag="AC16CB_16785"
                     /inference="COORDINATES: protein motif:HMM:NF024637.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR3344181.1"
                     /translation="MDIGSIFLILGLLVLVGLFIGRPLLEHQATVVSQEEHQYSSLLA
                     ERDRILNALQELDFDYTLGKIPEADYPGQRASLLTRGADILRQLDAFGVETSEEEAEA
                     QLEAVIAARRADIASNGGPGRILRTSPDDDLEALIASRRRVRSEKARGFCPQCGRPVQ
                     TSDRFCPNCGATIA"
     gene            complement(6961..7530)
                     /locus_tag="AC16CB_16790"
     CDS             complement(6961..7530)
                     /locus_tag="AC16CB_16790"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR3344182.1"
                     /translation="MEQSESLVTQTPERTRPQWGRVLVWVGLMGLLVILALGLKRSQQ
                     GPVSVGDRVPNFTLTTFDGAQINLEDLQGKVVVLNFWASWCKPCEQEAADLEAAWRFY
                     KPRGDVVFLGVDYVDTEPEALAYLKKFDITYSNGPDLGTRISQAFRIQGVPETYIIDQ
                     QNVLRFVQIGPFTSLEQIKGAIDPLLTGG"
     gene            complement(7532..>8013)
                     /locus_tag="AC16CB_16795"
     CDS             complement(7532..>8013)
                     /locus_tag="AC16CB_16795"
                     /inference="COORDINATES: protein motif:HMM:NF015853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HIR3344183.1"
                     /translation="MRRLIPLLALIFAFVLSGSALAQDGSPVAPSDDQVNAIAKQLYC
                     PVCENIPLDVCGTQACAQWRELIREKLSQGWSEPQIKQYFVDQYGDRVLATPPARGLN
                     WLVYLVPPLAIAAGVYILYRALKAWKQPTPLEDSAASSSSTQTDEYVRRLEEELKKR"
BASE COUNT         1982 a         2087 c         2160 g         1784 t
ORIGIN      
        1 taaagctcgg cagttaacac cactcgctga tgagctgggc tgcagcatgg cacaactagc
       61 cttagcatgg tgtctcaaga atcaacatgt gagtacggta atcacaggtg catctcgccc
      121 agaccaggta agtgagaatc tcggatcctt ggaggttgtt ggaaagctca cacctgatat
      181 catggaacgt atcgaggaga ttctcgacaa caagcctgag ccaatagagg atgagcgata
      241 aataaacttc gatattaccg agatcatcgc caacctagaa gcaactctaa acaagaacct
      301 aattcgaaaa gccatatcac cggtcacata tatctatccc tccccgaatt agggttggca
      361 tgatattgct cgcgtccccg ggtgacgaaa aacagtacac atagagtaaa aatattgcta
      421 atatgagagc gttcggaaac tcaattgagg ccattttcat ctctcttaat ggaaaaaacc
      481 aacatttgga gttgaaaaat cgacgcctat cacacgaaaa aatgtgcctt ccacaatcaa
      541 cagcaccgat ttttggtcaa ttgatagcca gtttggctga ttttccatgc caaatcacct
      601 gctgaagagc atctttgaca ttgaaaagcg ttgctaatcg ttgccaaatc gtctcatctg
      661 ttgtcggaaa tccagccacg attatcaatc tttttcgtaa agatagtttc cgaacgctct
      721 caataatatt taacccaagt tgctacccaa caagatagtt aatagaacag gcaagcacga
      781 agagagccac tttgagttca aaaccacggg aagtaacggc atgaatatgc ttcggcaaga
      841 gctgctcgat caagctacct gttgtttcaa taagcttgcg gtaagaagct ctcaagtagt
      901 gaacccaagg ggggaggggg cgcttagagt tcttcttccg caaagggata aaggagatgc
      961 cagcttggtt gatgacatct tctacctcat aatcgttata cgccttgtca ccggtgagct
     1021 tggcgccttc aggcaggtca aagtcataca gcttcaaggc actcgtatcg ctgtaactgc
     1081 ccggagtgag gaaaaactcg accggttgac cggtctcggt gatcatcaga tggatcttca
     1141 gaccataaaa gaaacgtctc ttgctggcct ggtagcctcg aaaatcttct tcttggtaaa
     1201 tctggcagcg ctgaatgcga tagttgtcgc aagccgccac tggaaagcta tcgatcacgt
     1261 agatcgagtt gctgttcaac tgcttccagg tttcacccag caggttgaac agggtcagaa
     1321 acaaatcggc attgcgatgc caacgacgat tgaaacgact ttttcccaac atattgggaa
     1381 cgtatccttc cgcctgaagc attcgacggg ctttctcgtg attcccacca taatccagag
     1441 cggctacaat cgcagtggtc atgacttccg catccgtcat ctggcattgt ggatcatctc
     1501 gatgatgaag ggctttcaac atatcgtcac aaaggcaata aactgctaca attcgggtgt
     1561 ccatgtgttc ctccggtttg ttgtggtttg ggtaccatca actttaccag atacacatgg
     1621 acttttttca tcaatcaccc aaggtggcaa cttgggttaa attaaataaa catcccgcag
     1681 gtgacaccta cgggatgtta aagagatcaa ttattttcac tatttatctc atccgctagg
     1741 caggatgttt tgggatcctc ctggaaggct ctcaggcagg agggcaagca aaagtaaagc
     1801 ctccgctcgc cgaagacggc tgtgggtgta tcgtcttcat aaaagataat ccctttgcag
     1861 gcggtgtaca cagggctgcc tgacttaggg gtgggcgcat tttgtggctc gctcatgctt
     1921 cgctttcatc ctgcaaatct tccagcaact cgagatcctg gctaaggctg cgactgcgca
     1981 aaatcaggct ggcaaggtaa ataagcatta ccccaaagat gacgacataa ccagcaatca
     2041 tgtaaccgct ggtttcggct gggtgatcgg gtaagtacgg cagaaatagc atctctttct
     2101 ctccttatat catcgctttc aacttaagtt gctcaacctt ctcggcaaac ctgcccaggc
     2161 ggatacggtg ccagagcaaa tcggcaaaga tgaaggtgaa tgtcagcaaa ctgaagagga
     2221 aagtttgcag catgagggga gtcatgtcaa aggcgccttc ggcaccgggc tgattggtgc
     2281 cgatcacaac cgggtggatg gtgcgaaaca gccgtgctga aaagaaggtc agggggacgc
     2341 tgagaaatcc gacgatggca tacacagccc caaagcgggc gcgccggtcg gggtcttcga
     2401 tgccctggcg cagcatcagg taagcggcgt agatgagctc catgatggtt gctgtggtga
     2461 gacgaggatc ccatgtccac caagtgttcc aaatggggcg tgcccagatt gaccctgtga
     2521 cgatattgat aaaagcaaat accattccga tttcaactgc tgagagtccg acaatatccc
     2581 attctcggcg cccgctgcgc aaataaacga tgccagctat agcagccacc agaaagctaa
     2641 gcatacctac ccagccagcc gcaacgtgga aataaaaaac tcgctgcact tggcccatca
     2701 ccgcttccat gggagcgtag aagaaaacca tgccggtggc aacgaccagc aggataacgg
     2761 tgacaacatc cagaattttc aataaagttg gtttgctacc cataaaatcc tccttgtttt
     2821 cggaatcaga gagtctagta tcgagattaa ttgccttgcg atagacgaat catgtatctc
     2881 gaatccctac tctcataact ctaatctcta ttcttccacc acataatcga acaccataaa
     2941 tgctacggca gtaaagatga catcataaac gatgagcagg ttgatccagg gcatgatttc
     3001 gtcgaagggg atgccttgca agtatcctcc gctgcccttg actgacgcaa tcacgaccgg
     3061 gatcacaacc gggaatagca ggatcggtag caggatgtcg cgggtacgtg tctgcactgc
     3121 catgctcgcc agcaacgtgc ccaccgccac atagcctacc gagcccaaca agatgaccag
     3181 caacaagcct ggcaggaaca aattcgtatt atacagcacg ctgtagatcg gcagcacaat
     3241 aatttcaacc aacagcatga agatgaggtt gcccagtgtt ttcccgaagt agatggcgct
     3301 gcgatctacc ggtgccagga gcaggccatc caggcagccg cggtcttttt cgattgccat
     3361 ggagcggttc aagcccagcg tgccagcaaa tgtgaaggtc acccacagca caccagaggt
     3421 gacgttggcg cgctctcggg catccagctc cagggcgaag ttaaagatca aaattaccaa
     3481 gagagcaaag acaagcatgg aggagagcag ctctcggctg cgcaattcgg cagccaggtc
     3541 tttccatacc acggctccca tggcgcgcag aaagctaaaa gcagctctac cctctagcgg
     3601 gtccttgtgg gccatcacct ggggggtatc gctcatgccg ttcccccgtt gaccgcctgg
     3661 cggtagaaag ccagcaagtc tcgcgtatca atttcttcac gctttaccga agcagtgatc
     3721 acaccccgcg aaagcacgtc gaagcgggat gccaggtcgg cagcccggga gaggtcgtgg
     3781 gaggtcatca cgactgtgcg gccacgagca gcgacctccc ccaggacgtt atccagcatg
     3841 ctgcttgcgt cttgatccaa cccggtgtgt ggctcatcga atagcatcac atccgggtcg
     3901 tgtaaaacag ctcgcccgat tgccaggcgc tgctgcatgc cgcgagagaa ggtgcgcacc
     3961 aggtctcggc ggcgaggcca taggccgacc aggttcagca cttcttcgat gcgcatagcc
     4021 agatcattaa tgccgtacat gcgcccgtaa aattgcaggt tctcttcagc cgtcagatcg
     4081 ccatacaaga ggggcaggtg agagacaacc cccaaacggc ggcgcactgc agctgcctga
     4141 gcgggcaggc gatacccggc gatgcgcact tctccaaggg ttggccgcga taatgaagcc
     4201 agaatgcgca aaaaagtggt cttgccagcc ccattcgggc cgagcaatgc cacaaattct
     4261 ccgggctcta catgaaattc cagcccgcgc aatacggcct tcaggccaaa gcgcttgacg
     4321 aggcggcgta cttcgatcat gggaattgag ggatggggtc aggggtatcc cttacccaat
     4381 gagctccttc aaccttctct tcaattcgtc tcgccgttgt tggtaggctt catcaggtag
     4441 ctgaccggcc tggtagaggt catccagtgc gatgatggca tccatgagac tatcgctggt
     4501 ctcaaagtcg gccgaatccg aagaatccaa agcttgctca gcatccagcg aatcggcgcc
     4561 gttaaggcgc gtgcgtcgat acagccacac accagccatt atgagcgcca aaccgaagac
     4621 agacagccca accagcaggc ttgtgtttga gctggtggtc agggagggag caccaatgga
     4681 gggtttcccg gtcagggtga gtctcaactc gtctcccgcg gctagcccgc ctccactgta
     4741 agtacggtat tgtgtacctt gtacgtcgcg aacgccactg tcctgcaata tgtcgctctt
     4801 gatcttgatg tcgccttcgg gcgcaaggat cacgatggca tccacgggca tggtcaccgg
     4861 gtgagccagt tccaggttgc ggtcataggg catctcaaag gcgaacaaca gctcataaga
     4921 tccaaaacct ggggggatgg agacagtatc accgaaaccg tctgcggttt taatatatcg
     4981 cccgcctaac gctccatcct ggaattccag tttcgttgct ccatctggga gggaaaagac
     5041 cacagacgct tctcctggct caggggctac cagggattta tcgctggggt tggacacgat
     5101 gtagagctcg ataacccgca cggtttttgc gtcaacaaac tcgaagaaca ggtgcagtcg
     5161 gtcggctttt aatattgagg catcgctggt ggtctcgtat acgacaatag gcagatccaa
     5221 gatgttgatg ccatcttggg cgatgccaat atcggaacca taggaggtgc gcccataatt
     5281 cactgtagcc agaaagacac gcccgtcagg catctgtatg tcagggaaga taaatgaacc
     5341 gtctgtttgt gtagtggtgg tctgggtcat tgctacctgc atattgtcaa agccatgcag
     5401 taggatttcc atttcttcag gcagctcgac tccagcggcg ctgatcacct tgcctgtcac
     5461 tgtgccaatg ccagcaggca cttccgcaat cggagtggca gacgcagaag tgggtgtggc
     5521 tgcttctccc ggagtgggag tctcagctac tgcgaccggc tcatctggtg cggtaaaggt
     5581 tagcgatcgt aaataagccg ccagggccca gatttcatcc tcggaaagct gcgcgccgaa
     5641 ggccggcatg gccgggggca ggccattttt ggttacctgg aagaagtcgt cagccgattt
     5701 ctctgccatg aatgcttgat cggtcaaatt ggttggcggt ttaggacccc cagctgcatc
     5761 ttgcccatcg cctttacctg tccccccatg gcatctagcg cagttggcct ggtaaagctc
     5821 ctgaccttga tcgaccagtt cagggggtgt ggtcagggaa aaagaatagg ccaccacatc
     5881 ccaacgctgg cgatcggata ggctattaaa tggcggcatg aacttctgca agttgccctg
     5941 agttacctgg gtataccagc gggccggcgt ggactggcga gctaactcaa tcgagccgat
     6001 ggcggctacc gggttaggaa gctgagctga ctggggcccg tccccaagac ctgtatcacc
     6061 gtggcagggg gcgcatttct cctcaaaaat ggctttgccg ctctctagat taggcggcac
     6121 cagcgggtaa aggggaccgc tgatcggcgc gggctgtgtc acggcgatgg gtggtctctg
     6181 ctctgagcct ggtggcggag tgatatcggc tgccagggag aaagagcaag ccgagatgat
     6241 cagagaaaac agcaatagtg caaatggaag aagaacaaaa cgagaagtta tttggcgtag
     6301 ccagcgaggt tgagattgtc tgtgcatatt aaatctatga tcaccgataa tggggtacta
     6361 ggctattgta gccccgcaat tggggcagaa acgatcggag gtttgtaccg gacgcccaca
     6421 ctgcgggcag aagccccggg ctttctcact gcgcacgcgg cgccggctgg caatcagggc
     6481 ttccagatcg tcatccggcg aggttcgcag gatgcgacca gggccaccat tagaggcgat
     6541 atcggcgcgg cgcgcggcga taacagcctc cagctgggcc tcggcctcct cttcgctagt
     6601 ctccacccca aacgcatcta gctggcgcag gatatcagcg ccgcgagtta gtagggaggc
     6661 tcgctgccca gggtaatccg cttcggggat tttgccaagc gtgtagtcaa aatccagctc
     6721 ttgtagggcg ttcaggatgc ggtcgcgttc ggcgagtaag ctggaatatt ggtgctcttc
     6781 ctggctaaca accgtggctt ggtgctccag gagaggacga ccgatgaaga gtccgaccag
     6841 gacgagtaaa ccaaggatga gaaagattga gccgatgtcc attttacagt gtttagagat
     6901 tagggatcat tgggttgcga gttgtagttt gtggtgtaca cggttcaagc aaactgctca
     6961 ctaccctcca gttaatagcg ggtcgatggc gcctttgatt tgctccagag aagtgaacgg
     7021 gcctatctgc acaaagcgta gcacgttttg ctgatcgatg atgtaggtct ctggcacgcc
     7081 ctggatgcga aaagcctgag agatgcgcgt gcccaggtct ggcccgttgc tgtaggtaat
     7141 gtcgaacttc tttaggtaag caagggcttc aggctcggtg tccacataat ctacgcccag
     7201 gaagaccaca tcgccgcgtg gcttgtaaaa gcgccaagca gcctccaaat cggcggcttc
     7261 ctgctcgcag ggcttgcacc atgaagccca gaaattgagc acgactacct tgccttgcag
     7321 gtcttccagg ttgatctggg cgccatcaaa agtggtcagg gtaaaattgg ggacccggtc
     7381 gcctactgac actggccctt gctgagatct cttcaaaccc agggcaagga taaccagcaa
     7441 gcccatcaac cctacccaca caagtacacg cccccattga gggcgagtgc gttcaggtgt
     7501 ctgtgttact aggctttctg attgttccat attacctttt cttcaactct tcttctaagc
     7561 gccggacgta ttcgtccgtt tgcgtgctgg atgatgatgc ggcggaatcc tcgagggggg
     7621 ttggctgctt ccatgcttta agagctcgat agaggatgta cacgccagct gcgatagcaa
     7681 gtggcggaac caggtagaca agccaattta gacctcgcgc cgggggagta gccaagactc
     7741 ggtcgccgta ctggtctacg aaatattgct tgatctgtgg ctcgctccac ccttggctga
     7801 gcttttcacg aatcaattct cgccattggg cgcaggcttg agtgccgcaa acatccaatg
     7861 gaatattttc gcacaccgga caatatagct gtttggcgat ggcgttgact tgatcgtcgc
     7921 taggcgctac agggctgccg tcttgtgcta aagcggaacc gctgagaacg aaggcgaaga
     7981 ttaatgcaag cagagggatc aagcgtctca tat
//
LOCUS       DBNGSX010000576         2611 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1930256, whole genome shotgun sequence.
ACCESSION   DBNGSX010000576 DBNGSX010000000
VERSION     DBNGSX010000576.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1930256"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="AC16CB_16800"
     CDS             complement(<1..252)
                     /locus_tag="AC16CB_16800"
                     /inference="COORDINATES: protein motif:HMM:NF014750.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HIR3344184.1"
                     /translation="MHGWTGKLLRIDLNNRSSTIEEIDEDILHRYLGGRGLGTYLVYT
                     EVPPATNPLSPENILAFCTGAMTGVRVPTGGRSSMSTLSP"
     gene            complement(266..928)
                     /locus_tag="AC16CB_16805"
     CDS             complement(266..928)
                     /locus_tag="AC16CB_16805"
                     /inference="COORDINATES: protein motif:HMM:NF012804.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR3344185.1"
                     /translation="MVIFNEYRLRLEITRVTRIVADQGLIRSSDGNISVRLDEDRFLV
                     TPSGLYKMAMEPDDPIVVNHQGEVLAGKQGLKPTSEMQMHLEAYHQRPDIKAVLHAHP
                     PYATALTITRLPFPTDFLPEVLIALGDVPTVPYATPGTTELAESIREHIKNHNSVLLS
                     NHGSISVGKTLEEALIALERLEHAAFTYHLARALGEPQSLSQEDLVILRAMGDKIRST
                     KM"
     gene            complement(928..2412)
                     /locus_tag="AC16CB_16810"
     CDS             complement(928..2412)
                     /locus_tag="AC16CB_16810"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HIR3344186.1"
                     /translation="MSHLSYAAVDLGAESGRVMLGNFDGKRLQIKEVHRFSNGGVRLG
                     KNLYWDVLRLWSEIKAGLSQTGAMSGGNLRSIGLDTWGVDFGLLDSDDTLIGNPYHYR
                     DARTDGMLEAVFEKVPRDEVYAQTGIQFMQLNSLYQLFAMVQAKNPALSNAKTFLNMP
                     DLFNFFLTGVKANEFTISTTTQCYNPVKRAWASNLLTILGIPTHIFGDIVKPGTVLGN
                     LRDTLADELGLPDTSIIASAGHDTASAIAAVPASTADYIYLSSGTWSLMGVELDEPMI
                     TAESLNANMTNEGGVGNNIRFLKNITGLWLVKECRRRWAQQGNKYSYAELTDMAVKAP
                     AFGPLIIPGNSKFLAPDDMPAAIQGSCRETGQTVPEDKGAIIRCALESLALEYRWVTE
                     QIARLTGKEHPTIHLIGGGTQNKLLNQFAASATGRTVITGPVEATAIGNILVQAIATG
                     EISSLAEGRAIVKNSFEVETYEPHETDAWDESYARYLELKKGSI"
BASE COUNT          618 a          671 c          657 g          665 t
ORIGIN      
        1 cggggagagc gtgctcatgc ttgaacgtcc gccagttggg acgcgcacgc cggtcatcgc
       61 cccagtgcaa aacgccagga tattctccgg gcttaagggg ttcgtagcag ggggaacctc
      121 ggtatacact agataggtgc ccaagcccct gccgcctaaa tatcggtgca gaatatcctc
      181 atcaatttct tctattgttg agctacggtt attgagatca atacgcagca attttcctgt
      241 ccaaccatgc atagcggata ttctcctaca tctttgttga gcggattttg tcacccatcg
      301 cgcgcagaat tactaagtct tcttgtgata atgattgcgg ctcacccagg gcgcgtgcca
      361 gatggtatgt aaaagcggcg tgttctaacc tttccagagc aatcaaagct tcttccagtg
      421 ttttgcccac cgaaatggat ccatggttgc tcaataaaac tgagttgtgg tttttgatgt
      481 gttcacgaat gctttcagca agttcagttg ttccaggtgt ggcataaggc acggtcggca
      541 catcgccaag tgcaatcaac acctcgggca gaaaatcggt aggaaatggc aatctggtaa
      601 tcgtcagagc ggtcgcatag gggggatggg catgtaaaac ggcttttatg tcggggcgct
      661 ggtgataggc ttccaagtgc atttgcattt cagatgttgg cttcagaccc tgtttcccag
      721 ccagcacttc gccctggtga ttgacgacaa tcgggtcgtc cggttccatt gccatcttat
      781 ataaaccact gggagtgacc agaaaacggt cttcgtccag gcgaaccgaa atgttcccat
      841 cacttgaacg tatcaatccc tgatctgcca caatccgcgt cacgcgtgta atttctagac
      901 gtaatcggta ctcattaaaa ataaccacta tatacttccc ttctttaatt ccagatagcg
      961 tgcataagat tcatcccagg catcggtttc atgcggctca tacgtctcca cctcaaatga
     1021 gtttttcaca attgcccgac cttcagccaa agaagaaatc tcacccgtgg caatggcttg
     1081 gacgagaata ttgccgatgg ccgtcgcttc caccgggcct gtgataaccg ttctacctgt
     1141 tgcactggcg gcaaactggt tgagcagttt attctgcgtg ccgccgccga taagatggat
     1201 ggtgggatgc tctttccccg tcaaacgggc gatttgttca gtcacccagc gatattccaa
     1261 tgccaagctt tctaaggcac aacggatgat tgcccctttg tcttcgggta cagtctgtcc
     1321 cgtttcccgg caagagcctt ggatcgctgc gggcatatcg tcgggtgcga ggaatttgct
     1381 gttgccgggg atgatcaggg ggccaaatgc cggagctttt actgccatgt ccgttaattc
     1441 ggcatagcta tatttgtttc cttgctgcgc ccacctacgg cgacattcct tcaccagcca
     1501 cagtccggta atattcttca aaaagcggat attgttccct acgccgcctt cgttggtcat
     1561 attggcgttg aggctctcag cggtaatcat cggctcatcc agctctaccc ccatcaatga
     1621 ccaggtgccg gagctaagat agatataatc cgctgtggat gctggtacag ccgcgatcgc
     1681 gctggcggta tcgtggccag cgctggcaat tatggatgtg tccggcaatc ccaattcatc
     1741 tgccagggta tctcgcaaat ttcccaaaac ggtgcccggc ttcacaatat ctccaaagat
     1801 atgagtaggg attcccagga ttgtgagtaa attggatgcc caagcgcgct tgacagggtt
     1861 ataacactgg gtggtggttg aaattgtgaa ttcgttcgcc tttaccccgg tcaagaaaaa
     1921 gttgaataaa tccggcatat tgaggaatgt ttttgcgtta gagagggccg gattcttggc
     1981 ctgtaccata gcaaataatt gatacaagct gttgagctgc atgaactgaa taccggtttg
     2041 ggcatacact tcatcacgag gcactttttc gaagacagcc tccagcatgc catctgtacg
     2101 agcgtcgcga tagtgatagg ggtttccgat cagggtgtcg tcggaatcca gcaggccaaa
     2161 gtccacgccc caggtatcca ggccgatgct acgcaggttg ccgccagaca tggcaccagt
     2221 ctgtgataat cccgctttga tctcgctcca caggcgtaga acgtcccaat acagattttt
     2281 cccaagccgc accccgccat tggagaatcg atggacttct tttatctgga gacgtttgcc
     2341 atcgaaatta cctagcatga cccggccgct ttcagcgccc aagtcaactg cagcatacga
     2401 aagatgagac ataacattgc tcccaaaagg gttccctgac aaagcccgat aactggcttt
     2461 tccagggaac cgaggagaga gaaaaacaga aattaactgt aaagcgggcc gaaattctgg
     2521 caggcgcgga aatctgcgct ttcaaaatta tccgtgccga aagcgttcca ggcgctgggg
     2581 cggaagattc tttctttgga tacgttgtgc a
//
LOCUS       DBNGSX010000577         2964 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_60229, whole genome shotgun sequence.
ACCESSION   DBNGSX010000577 DBNGSX010000000
VERSION     DBNGSX010000577.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_60229"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="AC16CB_16815"
     CDS             complement(<1..248)
                     /locus_tag="AC16CB_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3344187.1"
                     /translation="MTPAMFNILLALADGEKHGYAIMLEVEANTEGAVKMGPGTLYGS
                     IKRMLASGLIEESDERPDPALDDERRRYYRLSGLGQRAL"
     gene            complement(554..1249)
                     /locus_tag="AC16CB_16820"
     CDS             complement(554..1249)
                     /locus_tag="AC16CB_16820"
                     /inference="COORDINATES: protein motif:HMM:NF039623.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5995 family protein"
                     /protein_id="HIR3344188.1"
                     /translation="MISSEAPIVARMQSLIHQWEETSNPQAVFLSCYKMMTSNMLTSI
                     ERQEFMDPPWVTNLLHRFADHYFVALESYEKEPTSAPRVWQLAHDTTRDPQVLPLQNL
                     LLGINAHINYDLVITLVDLLSPEWNDHSDEQRASRYYDFCHVNDVIVRTIDVVQDQVL
                     EPAMPGMDILDKLLGSLDELLVSRLITSWREKVWHNVLSLLEATAQDEQWSLLQQIEE
                     EAVGLGKLISLRD"
     gene            complement(1297..2334)
                     /locus_tag="AC16CB_16825"
     CDS             complement(1297..2334)
                     /locus_tag="AC16CB_16825"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HIR3344189.1"
                     /translation="MNSHLNRIHHFKGNPREIGFAAGRLLGDKLAQNINHYIGSLEHS
                     KDMEELHTGALPWLRRLPERFQEEYEGLAEGANIPLQRLAEWGYLEACKSIQCSGTVY
                     LFENQAWVARNNDFYLPELWGYVTVREVDGRIPTINFPMEGDVFTPTGVNKEKLWLHY
                     NYLPVWDNPSPNKPHVPAYVFLTEALEICHTIRDVETFLNEMDRDGGMLLFAVDGKNN
                     EFALFDCMCSKHYRREPSDGWIVGTNHYCAVEDLTLGDDEDSANTINRFRRMESLMQD
                     LATSKTPPSLPADLIKILADDEIERRDVEVITVYSNVACPSTGEIWFTFGGHPAASQG
                     NWQKIDWPWKD"
     gene            complement(2354..2731)
                     /locus_tag="AC16CB_16830"
     CDS             complement(2354..2731)
                     /locus_tag="AC16CB_16830"
                     /inference="COORDINATES: protein motif:HMM:NF013384.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HIR3344190.1"
                     /translation="MTEPSGTMANQVALPVLTHPGDDVILNRESHAVWHETGASAANS
                     GVQFTAVGAKGIFTLDGFLEAIKPRGHVIYPPTTLVEIDNTHNRAGGVIFPDSVCTHP
                     THHSGSDTPFQPTWLSYAAEPKR"
BASE COUNT          724 a          738 c          705 g          797 t
ORIGIN      
        1 agggcgcgct gccccaggcc actcaaccgg tagtagcgcc ggcgctcgtc gtccagcgct
       61 gggtcagggc gctcgtcgct ctcttcgatc aagccactgg ccagcatgcg cttgatcgag
      121 ccgtatagcg tcccgggacc catcttgacc gccccttcgg tgttggcttc cacttctagc
      181 atgatggcgt aaccgtgctt ttcgccgtca gccagcgcca gcaggatatt gaacatagcc
      241 ggagtcatgg ggaggttatc ttttggtagg ggggaggtaa cagtcactga tgctccaaag
      301 acggaagtat atatatccgt gaacgatata tctattatgg atattttata tcgtatatat
      361 ccgattgtca agaggcaaat ccgacggata atgtactgcg cttgccatcg ccccctaaaa
      421 agttgctttt ttcaccaatt catcattagg accgagttgc gaaaaacaaa tcccgataaa
      481 atctgacaga gtgttcttca cgtagcatag cggtttgaag cgccaagttt cttctatatt
      541 gagacgggct gcgctaatcg cgcaagctga tcagcttgcc taacccgaca gcttcttctt
      601 caatttgctg aagcaaagac cattgctcgt cttgtgcagt ggcctcaagc agacttagta
      661 cattgtgcca cactttctcc cgccaacttg taatcaggcg cgaaaccagc aattcgtcga
      721 gagacccaag cagtttgtcg agaatatcca tccccggcat ggcgggctcc aacacctggt
      781 cctgaacaac gtcaatggta cggacaatga catcgttgac atgacaaaaa tcgtagtagc
      841 gcgaggcacg ctgctcgtcg gagtggtcat tccattctgg gctcagcaag tcaaccaacg
      901 ttatcaccag gtcgtagttg atgtgggcgt tgatgccaag cagcaggttt tgaagcggca
      961 gcacttgggg gtcgcgagtg gtgtcatgcg ccaactgcca gacgcgtggg gcagacgtcg
     1021 gttccttctc ataggattct agcgctacga aatagtggtc agcaaagcgg tgcagtagat
     1081 tggtgaccca tgggggatcc atgaattcct gtcgctcgat agaggtaagc atattgctcg
     1141 tcatcatttt gtagcagctc agaaacaccg cttggggatt ggatgtttcc tcccattggt
     1201 gaatgagcga ctgcattcgt gcaactatgg gagcttcgga agatatcact atttcctcct
     1261 atactttgta tcccttatct caataaaaaa ttcttatcaa tctttccacg gccagtcaat
     1321 tttttgccaa ttcccttggc ttgctgctgg gtgtcctcca aatgtgaacc agatctcacc
     1381 tgtgctcgga catgccacgt tagaatacac agtaattacc tctacgtcac gcctttcgat
     1441 ctcgtcatcc gccaggatct ttatgagatc ggcgggtaga cttgggggtg tcttcgaggt
     1501 agctaagtct tgcatgaggc tttccatacg tctaaatcga tttatcgtgt tggctgagtc
     1561 ttcatcatcg cccaaagtca ggtcttcgac ggcacaataa tgattggtcc caacaatcca
     1621 gccatcggat ggttcccgtc gatagtgttt tgagcacatg caatcgaaaa gagcgaactc
     1681 attgttcttt ccatcgacag caaataataa catgccacca tctctatcca tttcattgag
     1741 aaacgtctcg acatcgcgga tagtgtggca gatctccagc gcttcggtta agaacacata
     1801 ggcagggaca tgcggtttat taggggatgg gttatcccaa acaggcaggt agttatagtg
     1861 caaccacaat ttctctttat tgacgccagt tggcgtgaac acgtcccctt ccatggggaa
     1921 attgatcgtt ggaatccgtc cgtcaacttc tctaaccgtt acatagcccc ataactcggg
     1981 caaataaaaa tcattattgc gcgcaaccca ggcttgattt tcaaataaat acactgtccc
     2041 actgcactgt atagatttgc atgcctcaag atacccccat tcagccaggc gttgcagcgg
     2101 gatgtttgcc ccttctgcca gtccttcata ttcctcctga aatcgttccg gcaaacgacg
     2161 caaccagggt aaggcgccag tatgtagttc ttccatgtcc ttggaatgtt ctaggctacc
     2221 gatgtagtga ttgatgttct gtgcgagttt atcgccgagc aagcgcccag cagcaaatcc
     2281 aatttcacgt ggattgccct tgaagtgatg tatccgatta agatggctgt tcattgaaat
     2341 gaattctctc cgcctaacgt ttaggttccg ccgcatagct aagccatgtt ggctggaaag
     2401 gtgtgtcact acccgaatgg tgcgtgggat gagtgcaaac actatcaggg aagatgactc
     2461 ctcccgcccg gttgtgagta ttgtctatct caaccagggt ggtgggtgga tatatgacat
     2521 ggccgcgcgg tttgatggct tcgagaaatc catcaagcgt aaatatgcct ttcgccccaa
     2581 cggcggtgaa ctggacaccc gagttcgctg ccgaggcgcc agtctcatgc cagacggcat
     2641 gactttctcg gtttagtatg acatcatccc caggatgagt aagaactgga agggcaactt
     2701 ggtttgccat tgtgccagat ggctcagtca atagatgtat gtaaaactac cgaacatcat
     2761 tcctagtgac agaaagatcc agaatttatt ccttatcgaa tttaatgaaa tccacctatt
     2821 gtattgagta attatatcct ttttgtacct tttgtgaaat tttcactatc aaaattgaaa
     2881 ttttgatcga gaggaaatct ctcaaataat ccgaggattc gtacaggaat tctcgtctct
     2941 ccattttcca gacctttcca gact
//
LOCUS       DBNGSX010000578         2918 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1307642, whole genome shotgun sequence.
ACCESSION   DBNGSX010000578 DBNGSX010000000
VERSION     DBNGSX010000578.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1307642"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="AC16CB_16835"
     CDS             <1..501
                     /locus_tag="AC16CB_16835"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3344191.1"
                     /translation="YLVKDYPNAIINRTMSKAFGLAGFRLGYALADAELVSYFYRVRI
                     PWNVSLLALAGALAALDDQEDQQRKHRTTIEERRFLQDEINNIPGLRAYPSEGNFVLI
                     DASIIGKSSTEIRDEFIERGMFIRPMSPHHMKEGFVRVTVGTHEQNTRFLQVLRAYVA
                     ENLQVN"
     gene            complement(517..1641)
                     /locus_tag="AC16CB_16840"
     CDS             complement(517..1641)
                     /locus_tag="AC16CB_16840"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HIR3344192.1"
                     /translation="MVTRKMLFSPGPVLTSERVKASLIHPDLCHRRPDFEQVLHRVRE
                     NILRLFNADHEYTSVVISGSGTAANETALSSIIKDADEVLLIKNGEFGERLHDILNCY
                     RLRVHILEYPWGSPPELSDVRRYLVGNENIQWVCMVFHETSTGMLNPVHQIGELAYEF
                     QRRFYVDCVSAVGGEDIQVVRDHIDACSGVPNKAVGGLPGVGFVVARRASLPALEEVG
                     RRNIYLNLQKHIESADLHDQTPNTPSVTMIVALDEALQELLEEGLENRMRRYRECSHL
                     IRAEVRNMNLSILVPDHLASSTVTSVFLPDGIHVDEFIDELDRQGYVVYPGKRHLHQQ
                     NMFQIANMGQIQAQDCRKFLQVLNNTLKQFSLKTESADQK"
     gene            complement(1650..>2918)
                     /locus_tag="AC16CB_16845"
     CDS             complement(1650..>2918)
                     /locus_tag="AC16CB_16845"
                     /inference="COORDINATES: protein motif:HMM:NF018012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR3344193.1"
                     /translation="PESLVEKCLSSLPSQITFGARNPKKDLLAGHGLLPITRNGGGSD
                     LTLDLKTDELRPIYSQDLIDYFRLMDGLEHIDFIAPVYARDFPEANRDVKVFELMIAN
                     TDKHMHMRAYSKKSLEIILEMATIVAGSKESLKKRPVLSLLEAPLSPLTFLDITVDAL
                     FLCGEFGIPLELCCMPIAGGTGPVTLAGSVTLANAEFLAGVVISQLYRPGAPLEYAPR
                     CMIMDMKTTVGLTGSIEGAMMAAAGAQLAREKYNAPISLHGPWTDSVLPDEQSNLERT
                     NFAFLAGLAGTNVFSGAGMIEQGKTFSHLQLVMDDEIHAMVKLVLGGMSTTQDHLAVE
                     AIERAGPGGNFLIDPLTLKYFKTERFFPSLLLRETRSSWVSKGGKSYRERAREKVFEI
                     LKHHQPNPMEEEVMNEISAIVKRSEKELEG"
BASE COUNT          696 a          756 c          726 g          740 t
ORIGIN      
        1 taccttgtca aagattatcc gaacgcgatc atcaaccgga cgatgtcgaa agcttttggt
       61 ttggcaggct tcaggctggg gtatgccctc gccgatgcgg aattggtgag ctatttttat
      121 cgagtgagaa tcccgtggaa cgtgagcttg ttagcgctgg ctggcgcgct ggccgcgctg
      181 gacgatcagg aagaccaaca acgaaagcac cggacgacaa tcgaagaacg ccgttttctc
      241 caggacgaaa taaataatat ccctgggttg cgagcctacc catctgaggg gaactttgtt
      301 ctgatcgatg ccagcataat cggcaaaagc tcaacggaaa ttcgggatga gtttatcgag
      361 cggggtatgt ttatccgccc aatgtctcct caccatatga aggagggatt tgttcgggta
      421 accgtgggta cccacgagca aaatacacgc tttttgcagg ttttacgcgc atacgttgct
      481 gaaaatttac aggtaaactg atatcggcga acggccttat ttttgatcag cgctttctgt
      541 tttcaggctg aactgtttca aggtgttgtt cagcacttgt aaaaacttgc ggcaatcttg
      601 cgcctgtatc tgtcccatgt tggcgatttg gaacatgttt tgctggtgca agtgccgctt
      661 gcctgggtaa accacataac cttgacgatc caactcgtcg ataaactcgt ccacatgtat
      721 gccatccggc agaaagacgg atgtcacggt actagaggcc aggtgatcgg gaactaaaat
      781 actaagattc atatttcgga cttcagcacg gatcaggtga gagcattcgc gataacgacg
      841 catgcggttt tccagtcctt cttccaaaag ctcctgcaag gcctcgtcca gggctacgat
      901 catcgtaacc gatggtgtgt tgggggtttg atcatggagg tcggcgcttt cgatgtgttt
      961 ctgtagattc aggtaaatat ttcttctgcc cacttcttcg agtgcaggga ggctggcgcg
     1021 cctggcaacc acaaatccca caccgggcaa gccgccaacc gctttgtttg gaacaccact
     1081 gcaggcatcg atatgatccc gcaccacctg aatatcttct cccccgaccg cgctgacgca
     1141 gtccacgtaa aatcttcgct ggaactcata cgccaattcg ccaatctggt ggacggggtt
     1201 tagcattccg gtgctggttt cgtggaaaac catgcaaacc cattgtatat tctcgttccc
     1261 caccagatac ctgcggacgt cgctcagctc cggcgggcta ccccagggat attccaggat
     1321 gtgtacacgc aaacggtaac aatttaagat atcgtgtaat cgctcgccga actcgccatt
     1381 ttttatcagc aagacctcgt cagcgtcctt gatgatcgac gatagtgctg tctcgttggc
     1441 tgcggtgcca gaaccactga tcacgaccga agtgtattca tggtcggcat taaacagccg
     1501 caaaatattt tcgcgcaccc ggtgtaaaac ttgctcaaaa tctggacgcc ggtggcataa
     1561 gtctggatgg atcagagatg ccttcacccg ctccgaagtt agaacgggac ccgggctgaa
     1621 caacattttt cttgtaacca tatttatgat caaccttcca gttctttctc ggatctctta
     1681 acgattgccg atatttcgtt catcacctct tcctccatgg gattgggttg gtgatgtttc
     1741 aagatctcaa acaccttctc ccgagcacgt tctcgataag acttaccgcc ttttgatacc
     1801 caggaagacc ttgtttcgcg taatagcagg cttgggaaga agcgctcggt cttgaagtat
     1861 tttagtgtca gtgggtcaat caagaagttt cctcctggcc cagctcgctc aattgcctcc
     1921 acggctagat gatcctgtgt agtggacata ccacctaata ccagcttgac catagcatga
     1981 atctcatcat ccatcacaag ctgaagatgg ctgaaggttt ttccttgttc gatcatgcca
     2041 gctccagaaa agacgtttgt cccagctagg ccagccaaga aggcaaaatt tgttcgctcg
     2101 agattcgatt gctcatccgg gagcacagag tctgtccagg ggccatgcag gctgatcggc
     2161 gcgttatact tttcgcgcgc cagttgagca cccgcagcgg ccatcatcgc accttcgatg
     2221 gaaccggtta atcccacagt agtcttcatg tccatgatca tacaacgggg agcatattcg
     2281 agaggcgcac ctgggcgata taactgcgag attaccacgc cggccaggaa ctcggcgttt
     2341 gccagggtca cactacccgc cagggtcacc ggacctgtgc caccagcgat gggcatacag
     2401 cacaattcca gcgggatgcc gaattcacca cacaggaata gagcatctac agtgatatcc
     2461 aagaaagtga gcgggctgag cggtgcttct agcaagctta atactgggcg ttttttcaga
     2521 ctttctttgc tccctgccac gatagtagcc atctcgagga tgatctcaag gctcttcttg
     2581 ctataggcgc gcatgtgcat atgtttatcg gtgttggcga tcatcaattc aaacaccttc
     2641 acatcacggt ttgcttccgg aaaatcccgt gcgtacactg gtgcgataaa atcgatgtgt
     2701 tccaacccat ccatcaggcg gaaataatca attagatcct gggaatagat gggtctcagt
     2761 tcgtcggtct ttaaatccag ggttaaatcg ctaccgccgc cattgcgtgt gatgggtaag
     2821 agaccatgcc cagccagcaa gtctttcttc ggatttcgcg cgccaaaggt aatctgggat
     2881 ggcaaggagg aaagacactt ttccaccagg ctttcggg
//
LOCUS       DBNGSX010000579         8631 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_130045, whole genome shotgun sequence.
ACCESSION   DBNGSX010000579 DBNGSX010000000
VERSION     DBNGSX010000579.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8631)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8631)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8631
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_130045"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="AC16CB_16850"
     CDS             <1..394
                     /locus_tag="AC16CB_16850"
                     /inference="COORDINATES: protein motif:HMM:NF013767.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_function: GO:0043424 - protein histidine kinase binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HIR3344194.1"
                     /translation="GEADEDSASVAGMAVLDQGALNNLLSLLGGEFSYLVELVDSFLE
                     DAPKLLADLNQYVAAGDPEGIRRIAHGLKSNGADLGAIAFSDLCKDLEMIGKSGSLDG
                     SADLLARINAEYAKVEAALKDIQREGQI"
     gene            391..1800
                     /locus_tag="AC16CB_16855"
     CDS             391..1800
                     /locus_tag="AC16CB_16855"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3344195.1"
                     /translation="MIEEHGHILVVDDNRMNRMKLSISLEQQGYTVGLAEDGQQALDI
                     LERQSFDVVLLDIIMPGLDGYQVLERIKGDPVMRDIPVIVISALDEIDSAVRCIEMGA
                     EDYLPKPFNPVLLRARLNASLQKKKLRDLEKAYLQQEVMLRQSEKLATIGKLSAGMAH
                     ELNNPAAAAQRGAEQLKDAFLSLQKIHLKLVELDLTKEQIETLLALNGLAHEKAAKPA
                     EVDSLTRSDREYDLEVWLEEQGVENAWEIAPTLVNLGYGRGDLNKLSTDFPPSEFKTI
                     VAWLHLTYTIYSLQEEIGHGSRRVSEIVKALKAYSYMDQAPIQNVDIHEGLDNTLVIL
                     RSKLKPGIKVHRDYAADLPKIQAYGSELNQVWTNLIDNAIDAMDGVGEITLRTRHDEQ
                     WVIVDVIDNGPGIPEGIQSKLYDPFFTTKPPGKGTGLGLNISHNIIVHKHQGKITIDS
                     QPGHTRFEVRLPLEYVPVE"
     gene            1873..3531
                     /locus_tag="AC16CB_16860"
     CDS             1873..3531
                     /locus_tag="AC16CB_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012407375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3344196.1"
                     /translation="MAKPIILAVDDEPVVLNSVERDLRQKYGREYRVMKSESGEMALA
                     ALKQLQARGESVALFIADQRMPEMNGTQFLEQAIELFPEAKKVLLTAYADTEAAIRSI
                     NRINLDYYLMKPWDPPQENLYPVLDDLLADWKVSASPSYDGIRVAGALWSPQSHAVKD
                     FLARHQIAYQWLDVDSNPDGRALADSYSDGAAKLPVVSFPDGSVFIEPSLQDMANKVG
                     MQTRAEARHYEIIIIGAGPAGLSAAVYGASEGHNVLVIEKHAPGGQAGNSPKIENYLG
                     FPAGLSGMDLARRAITQAKRFGAEILSATEVVNVRIQDQYRIVTLADGTELSCKALLL
                     ASGATFRRLDVPGVEKLTGSGVYYGAAYTEARYYQDQHVFVIGGANSAGQGAMFLSKF
                     ASKVTMLIRRDTQWSSKYLVDAEAANPKIERLFNTEVTEVVGEPGHVSHIVTLNNITN
                     EVRNLEGAAVFIFIGAMPNSGIVRDLVSVNDKGFIITGPDLIRNGKRPKGWTLKRDPL
                     MLETSVPGIFAAGDVRLGTNQRVASATGEGGVAIAAIQQYLSTI"
     gene            3667..4692
                     /locus_tag="AC16CB_16865"
     CDS             3667..4692
                     /locus_tag="AC16CB_16865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3344197.1"
                     /translation="MNRVPKVSILLPCYNAAETLDEALDSLALQSLRDYELVAVDDGS
                     NDSTPEKLRQRAENDPRLRVLALPHGGIIAALNAGLAACRGEYVARMDADDRAHPARL
                     EKQLIYLEDHPEVAVLGCRVAGFPPDQVREGFRIYLQWQNALLSDAEIRREMFVESPV
                     AHPSVMVRRQWLEEVGGYQEHGWAEDYDLWLRLYLAGARFAKLPEILLEWRERPERLT
                     RRDGRYSLENFLRAKAFYLGKGPLAGRDAVMIWGAGMIGRRLSKHLLRQDVPLVAFID
                     IDPRKIGRLRRDRPILSPDELPDWWGRYQNPVVLAAVGARGARALIRERLNAMSLCEG
                     RDWWGVA"
     gene            4711..5127
                     /locus_tag="AC16CB_16870"
     CDS             4711..5127
                     /locus_tag="AC16CB_16870"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF046335.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIR3344198.1"
                     /translation="MDLNQHIQPGLFREDTYFVEEHQSAIHVGSGSLRVLATPWMIAF
                     MEKTARLLLSERLPAGYSSVGTHVDVRHLAPSAVGARLRVRAEVLSVDALKVAFAVQA
                     WDEQEQIGAGQHQRVVIDEERFLRRIAAKTSKAEGS"
     gene            5124..6236
                     /locus_tag="AC16CB_16875"
     CDS             5124..6236
                     /locus_tag="AC16CB_16875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3344199.1"
                     /translation="MTLPDTPLHSVLYDGWSLIYQPNSPQSLHLLAILSCRLEGFQGF
                     LALPGEPPDWLPPVETTRVIFTANMPRNRLVWEQRILPGLRRELEADLLHLTSPTPPL
                     FGPAVNVVSPSGFGGSHRVGGFSSRLRQAVSQGGMARLAGLFWPDDLPDPKGMGAVYR
                     LPPAIPVEFFDLGSKPGDRQLGESLDLPEAYVLYHGPADETALRRLLNAWSWVAGSVG
                     EMYPLLAIGLEDESRKKLDVLAASFDLSETVRSLPAMPPKDLGLIYQRCVAVLHPGDD
                     SPWGGPVRLALASGKPVVSQVSEHMDALVGPAAYLVKGGEARALGAALITVLVEEEIA
                     ERLSQLGRQRAEAWSLETFSQGLLAAYQDMLSKQNK"
     gene            complement(6279..7073)
                     /locus_tag="AC16CB_16880"
     CDS             complement(6279..7073)
                     /locus_tag="AC16CB_16880"
                     /inference="COORDINATES: protein motif:HMM:NF028519.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR3344200.1"
                     /translation="MNRRHIYASLYLLGLLIVLVGLGAPALAAPNLQLTPFPTPTPGP
                     DGRIVYFVQEGDTLLRISLISGITVDELRGLNNLTGDNIVVGQELLLGLGGPSQPSPS
                     PGPSPTVTPPLPTPSPQPGLANLCVILYDDINGDSIRQEEEPFIPGGAISVNNRSGSV
                     AETVQTPSGLEPYCFEELPEGEFSISVAIPEGYNPTTVTNYAMRLNAGDETFLDFGAQ
                     ANTETIAEAPTPTGSGRSPILGIIGGLFLLAGAGLAFFASRLMRSK"
     gene            complement(7135..8307)
                     /locus_tag="AC16CB_16885"
     CDS             complement(7135..8307)
                     /locus_tag="AC16CB_16885"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HIR3344201.1"
                     /translation="MHRLVALFLLTSFLLSACQPQRVANPITDDPSPTNSTPTSSSPP
                     PLSLTPLAPILSTSLPSTNTPSPSPSPTPTQAPRSPTPPPTTTLLFTGVIVPARCVQA
                     AIDASGDPDYPYDEVREVITKADLAIGTLNATMSDYPPHTGCKSTYVLVGNPENADAL
                     GRAGFDLMSVATNHIKNCGLTNCGDRAFFDTLDNLRRVGIQPIGAGANHTEAMQPVVV
                     TLNGVRFGFVSLGQIEPMAFATDERPGIAVLNEDNLRSAIAAARKVSDVVIAMPHWGP
                     EDVPTPNWSQRGLAQQAVEAGADLVVGNHTHVVQAMQQIDGVQVFYGLGNFVFDQWPR
                     DHQQGVILLVRFIGTRYDGYEFIPTHVDRDGRVHMANPDEADEILERIRQASEKLK"
     gene            complement(8316..>8631)
                     /locus_tag="AC16CB_16890"
     CDS             complement(8316..>8631)
                     /locus_tag="AC16CB_16890"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR3344202.1"
                     /translation="LIPELALNPQEFNRKFKDNPVKRAKRRGYLRNVAVALGNTHHTD
                     AISPLIQALEGDAEPLVRIHAAWALGQIGGKEARLGLQSALQSEADNEVRAEILVALE
                     MV"
BASE COUNT         1848 a         2180 c         2684 g         1919 t
ORIGIN      
        1 aggggaagca gatgaggatt ctgcctctgt ggctggcatg gcggttcttg accaaggtgc
       61 gctcaacaat ttgctgtcat tgctcggtgg ggagttcagc tatctggtgg aactggttga
      121 ctcattcctt gaagatgctc cgaaactact ggctgatttg aatcagtacg ttgccgctgg
      181 ggatccagag gggatacgaa ggatagcaca tggacttaaa tcaaacgggg ctgatcttgg
      241 cgccattgct ttctccgact tgtgtaaaga cttggaaatg attgggaaat caggctcgct
      301 cgatggatcg gcagatttgc tggctcgaat caacgccgag tatgcaaaag tcgaagcagc
      361 ccttaaggat atccagcggg aaggccaaat atgatagaag aacacggtca tattttggtc
      421 gtagacgaca accgcatgaa tcggatgaag ttatccatta gcctggagca acagggttat
      481 accgttggtc tggctgaaga tggacagcag gcgttagata ttctcgagag acaatcattc
      541 gatgtcgtgc ttctggatat catcatgccg gggttagatg gttatcaggt gctagaacgc
      601 attaagggcg atccggttat gcgtgatatt ccagtcattg tcatctcggc attggatgag
      661 atcgacagcg ccgtgcgctg tatcgagatg ggggcagaag actacctgcc caagccgttt
      721 aatccggtat tgctgcgagc gcggctgaac gccagcctgc agaagaaaaa gttgcgtgat
      781 ctcgaaaaag catacttgca acaggaggtc atgctgcggc agagtgaaaa actggcgaca
      841 attggtaagc tcagcgcggg tatggctcac gagctgaaca atccagccgc ggctgcccaa
      901 cgcggcgcag agcaattgaa ggatgcattc ttgagcctcc agaaaatcca tctaaagctg
      961 gtagagttgg acctcaccaa ggagcaaata gagacgctct tggcgctcaa cgggctggca
     1021 cacgaaaaag cagctaaacc cgcggaggtc gattcactga ctcgcagtga tcgcgaatat
     1081 gatttggaag tctggctcga ggagcaaggg gtagagaatg cctgggaaat cgcgcctacc
     1141 ctggtgaacc ttgggtatgg gcgcggtgac ttaaataagc tctcaacgga tttccccccg
     1201 tctgagttca agactatcgt tgcatggctg catctaactt atacgattta cagcctccag
     1261 gaagaaattg gacacggttc aagacgcgtc tccgaaattg tgaaagctct gaaggcctat
     1321 tcctatatgg atcaggcacc catccagaat gtggacattc acgagggatt ggataacact
     1381 cttgtcatac tgcgcagcaa gcttaaaccc ggcatcaaag ttcaccgcga ttatgcagcc
     1441 gacctgccga agatacaggc ctatggcagt gaactcaacc aggtctggac aaacctgatt
     1501 gacaacgcca tcgacgcgat ggacggggtg ggtgagatca ccctgcgcac gcggcatgac
     1561 gagcagtggg tgattgtcga tgtgatcgat aatggtccgg gcattccaga ggggatccaa
     1621 tcgaagttgt atgacccatt cttcaccacc aaaccgcccg gtaaagggac tggtttgggg
     1681 ttaaatatca gtcataatat cattgtgcat aaacatcaag gaaagattac gattgattcg
     1741 cagccgggcc atacccgttt tgaggtgcgg ctccccctcg agtatgtgcc ggttgagtaa
     1801 aatggcgctc tcggggttat atgccgggag agcaacaaga aattttgact tttagtaagg
     1861 aataagatac ctatggcaaa acccatcatt cttgcagttg atgatgaacc agttgtactg
     1921 aattcagtgg aacgcgactt gaggcaaaaa tatggtcgcg agtatcgtgt gatgaaatcc
     1981 gaatcgggcg agatggcgct tgccgcgctt aaacagttgc aggcgcgcgg cgaatcagtc
     2041 gccctcttta tagccgatca gcgcatgccc gagatgaatg gaacccaatt cctggagcag
     2101 gcgatagagc tgtttccaga ggcgaagaag gtgttgctga ccgcctacgc agatacagag
     2161 gcggctattc gctccatcaa ccgcattaac ctggattact acctgatgaa accttgggat
     2221 ccacctcagg agaatctcta cccggttctg gatgatctgc ttgcggattg gaaggtgagc
     2281 gcatccccct cctacgatgg catccgcgtt gcgggagctt tgtggtcgcc ccagtcacac
     2341 gccgtcaagg acttcctcgc ccggcaccaa atcgcttacc agtggctgga cgtggattcg
     2401 aatcctgatg gacgcgccct ggcagactcc tatagcgatg gcgccgccaa acttccggtt
     2461 gtttcctttc cagatggcag cgtttttatc gaaccgtcct tgcaggatat ggccaacaaa
     2521 gtcggcatgc agacaagggc ggaagcccgc cattatgaga tcattattat cggcgctgga
     2581 ccggcgggtt tgtccgccgc cgtgtacggg gcttccgaag gacacaatgt cctggtgatt
     2641 gagaagcacg cgcccggtgg gcaggctgga aatagcccga aaatagaaaa ctatctcggt
     2701 tttccagctg gattatccgg catggacctt gctcggaggg cgattacaca ggccaaacgc
     2761 tttggcgcag agatcctctc agcgactgag gtggtcaacg tgcgcatcca ggaccagtac
     2821 cggattgtca cgcttgcaga cggaacggag ctctcgtgca aagctctgtt gctggcgagc
     2881 ggggcgacct tcaggaggct ggatgtgccc ggtgtggaga aattgacggg ctctggggtt
     2941 tattatggtg cagcttatac cgaggcgaga tattaccaag accaacatgt gttcgttatt
     3001 ggcggggcaa attcagcagg acagggcgca atgttcctga gtaaattcgc cagcaaagtg
     3061 accatgctca tccgccgcga tacccaatgg tcctcgaaat atcttgttga cgcggaagcc
     3121 gccaatccga aaattgagcg gctattcaac acagaggtca ctgaggtggt cggcgagccc
     3181 ggtcacgtga gccacattgt caccttgaat aacatcacca atgaggttag gaacctggaa
     3241 ggggcggcgg tgttcatttt tatcggcgct atgccgaata gcgggattgt gcgcgacttg
     3301 gtttcggtca atgataaagg ttttatcatt acaggacccg atttgattcg caatggcaag
     3361 cgacccaaag gatggacact caaacgcgat cccctcatgc tggaaacaag cgtgcccgga
     3421 atttttgccg ccggcgatgt tcgcctgggt acaaatcaac gcgtggcctc cgcgacgggc
     3481 gaaggtggcg tcgccattgc cgccatccag cagtacctgt ccaccatcta ataccatctc
     3541 cgcaattttt agtagctact cacctattcc cccttccttg gctggaaagg ggatgactgc
     3601 ccgtttttcc ggtatttctc ccatccaggg tatgagtaat aaccgatttt aggtacaatc
     3661 gggacgatga accgtgtccc gaaagtatcc atcctcctgc cgtgctacaa cgcagctgag
     3721 acgctggacg aagccctgga tagcctggca ttacagagcc tgcgggatta tgagctggtg
     3781 gctgtggatg acggctcgaa tgatagcacg ccggagaagc tgcgccagcg cgctgagaat
     3841 gacccgcgcc tgcgggtcct ggcgctgccg catgggggta tcatcgctgc gctgaacgcg
     3901 ggtctggcag cttgccgggg tgagtacgtc gcccgtatgg acgccgacga tcgcgctcac
     3961 cctgctcggc tggagaagca gctcatatac ctcgaggatc accctgaagt ggctgtgttg
     4021 ggctgccggg tagcgggatt cccgcctgac caggtgcgcg aaggattccg gatttacctg
     4081 cagtggcaga atgctttgct gagcgatgcc gagatacggc gtgagatgtt tgtcgaaagc
     4141 cctgtggcgc accccagcgt gatggtccgg cgacagtggt tggaggaggt tggcggttat
     4201 caggagcacg gctgggcgga ggattatgac ctgtggttgc gcctgtatct ggcgggggcg
     4261 cgcttcgcca agctacccga aattctcctc gagtggcgcg agcgaccgga gcggctgacg
     4321 cgccgcgatg ggcgttactc attggagaac ttcctgcgtg ccaaggcatt ttacctgggg
     4381 aaagggccgc ttgcaggcag ggatgccgtg atgatctggg gagcgggcat gatcggtcga
     4441 cggttgagta agcacctgct tcgccaagat gtcccgctgg tggcctttat tgacatcgac
     4501 cctcgtaaga tcgggcgcct gaggcgagac cgccctatcc tttctcctga cgagttgcct
     4561 gactggtggg gacgctacca aaaccccgtt gtgctggcgg cggtgggggc gcgtggggcg
     4621 agagcgctga tccgggagcg cttgaacgcc atgagcctgt gcgaggggcg ggattggtgg
     4681 ggggtggcgt aaaggagata ttggacaagt atggatctta atcaacatat ccaacctggt
     4741 ctgttccgcg aggacaccta ttttgtcgaa gagcatcagt ccgccatcca tgtgggtagc
     4801 ggctcgttgc gcgtgctggc tacaccctgg atgatcgcct ttatggaaaa aacagctcgc
     4861 ctgttgctga gcgagcgcct gcctgcgggt tattccagcg tgggaactca tgtggatgtg
     4921 cgccacctgg ctccctcggc agtgggggcg cgtctgcgag tgcgtgctga agtgctgtcg
     4981 gtggatgctc tgaaagttgc ctttgctgtc caggcttggg atgaacagga gcaaattggt
     5041 gcggggcagc accaacgcgt agtgatcgat gaagagcgct tcctgcggcg tatcgcggca
     5101 aaaacgtcaa aagcagaagg ttcgtgacct tgcctgacac accgcttcat tccgtcttgt
     5161 atgatggctg gtcactgatt taccagccaa atagcccgca atctctgcac ttgctggcta
     5221 tcctatcttg ccgtttggag ggcttccagg gcttcctggc attgcccggg gagccgcccg
     5281 actggcttcc acccgtcgaa actacccggg tgattttcac cgccaatatg ccacgcaacc
     5341 ggctagtgtg ggagcaacgc atactgcctg gtctgcggcg tgaattggag gcagacctcc
     5401 tgcatctaac ttcgcctacc ccgccacttt ttggacctgc agtcaatgtg gtcagcccga
     5461 gcggatttgg cggcagccac cgcgtaggcg gcttcagctc ccgactgcgc caggcggttt
     5521 cccagggagg catggcgcgc ctggctggtt tgttttggcc agatgatttg ccggatccaa
     5581 aagggatggg agcggtttac cggctacccc ctgcgatccc tgtcgaattt tttgacctcg
     5641 gttcgaagcc aggtgaccgc cagctgggtg agtcgcttga cctgccggag gcctacgtgt
     5701 tgtatcacgg accggcagat gaaaccgccc tgcgccgctt gttaaatgcc tggagctggg
     5761 tggctggctc ggtaggtgag atgtaccctc tattagcgat tggattggag gacgaaagtc
     5821 ggaaaaagct ggatgttctg gctgcgagct ttgatcttag cgagacagtg cgttccctgc
     5881 cggcgatgcc tcctaaagac ttggggctca tataccagcg ctgtgtcgct gtcttacatc
     5941 ccggcgatga ctctccttgg ggcgggccgg tgcgtttggc tctggcgagc gggaagccgg
     6001 ttgtgtctca agtgagcgag cacatggacg cgctggtcgg tccagcggct tatcttgtga
     6061 agggtggtga ggcgcgtgcc ctgggggcgg ctttaatcac tgtgctggta gaagaagaga
     6121 ttgcggagcg gctatcccag ctgggacgcc agcgggctga ggcctggtcg ctggagactt
     6181 tttcccaggg gctgttggcg gcatatcagg atatgcttag caagcaaaac aaataacccc
     6241 acaatgagaa attgtggggt tttttcatgt attgtatttt atttgcttcg cattaagcgg
     6301 gatgcaaaga acgccagccc agcacctgct aataggaaca gtccaccaat gatgcccaat
     6361 ataggcgatc tgccgctgcc ggttggagtg ggcgcctcgg cgatagtctc tgtattggct
     6421 tgcgctccaa aatccaagaa ggtctcatca cctgcgttca ggcgcatggc gtaattggtc
     6481 acggtggtgg ggttgtagcc ctctggtatt gcgaccgaga ttgaaaattc tccttcgggc
     6541 agttcctcga agcagtaggg ttccagcccg ctgggtgtct ggacggtctc tgcgacgctc
     6601 ccagagcgat tgttcacgct gatggcgcca cccgggataa atggctcttc ctcctggcgg
     6661 atagagtcgc cattgatgtc gtcatagaga atgacacaca ggttcgccag cccgggctgt
     6721 ggtgaggggg taggcaaggg tggtgtgacg gtggggctgg ggcccgggga gggtgaaggc
     6781 tgggatgggc cgcccagacc gagtagcagt tcttgcccga caacaatatt atccccggtg
     6841 agattgttca acccgcgcag ctcgtccacc gtgatcccgg agatgaggga gatgcgtaac
     6901 aaggtatcac cctcctggac aaagtagacg atgcgcccgt ccgggccagg tgtgggggtg
     6961 gggaaggggg ttagctgcag gtttggcgct gccagggccg gcgcgcccag gccaaccagg
     7021 acgattaaca aacctagaag gtagagactg gcgtagatgt gtctccggtt cataaagaga
     7081 ttataacgga aattatcacc ggagtatgcc tgtattaggg tagttgccta tgaactattt
     7141 gagtttttca ctcgcctgac ggatgcgctc tagaatctcg tcagcctcgt ctggatttgc
     7201 catgtggacc cgcccgtcgc ggtccacatg ggtgggtatg aactcgtagc catcgtagcg
     7261 agtgccaatg aagcgcacca gcagaatcac accttgctgg tggtcgcgcg gccattggtc
     7321 gaagacgaaa tttcccagcc cgtaaaagac ctgcacgcca tcgatttgct gcattgcctg
     7381 tacgacgtgg gtgtggttgc cgaccaccag gtcagcgccc gcttcaaccg cctgttgggc
     7441 cagcccgcgc tgcgaccagt tcggtgtggg aacgtcctcc ggcccccagt ggggcatggc
     7501 aatgaccaca tctgagactt tgcgggcagc ggcaattgcg ctgcgcaggt tatcctcatt
     7561 gaggacggcg atgcccggtc gttcgtcggt ggcaaaggcc attggctcga tctgtcccag
     7621 cgacacgaaa ccaaagcgca cgccgttgag tgtcactacc accggctgca tggcctcggt
     7681 gtgatttgcc ccggcaccta tcggctggat acccacccga cgcaggttgt ccaacgtgtc
     7741 aaaaaaggcg cggtcaccgc agttggtcag gccgcagttt ttaatgtgat tggtagccac
     7801 gctcatcagg tcgaaaccag cacgacctaa ggcgtcggcg ttttcggggt tgcccaccaa
     7861 aacataggtc gatttgcagc cggtatgcgg cgggtagtcg ctcatggtgg cattgagcgt
     7921 gccgatcgcc aggtctgctt tggtgatgac ctctcgaacc tcatcgtagg ggtagtctgg
     7981 gtctccggaa gcatcgatgg cagcttgaac gcagcgcgct ggcacgatca cgccggtaaa
     8041 gagcagcgtt gttgtgggtg gtggagtggg gctgcggggt gcctgggttg gagtgggaga
     8101 gggagaagga gaaggggtgt tcgtggacgg aagagaggtt gagaggatag gggctaaggg
     8161 agtaagggat aagggaggag gggatgagga tgtgggggtg gaattcgttg gcgatggatc
     8221 gtcagttata gggttggcta ctcgctgagg ctggcaggct gatagcagga atgatgtgag
     8281 aaggaagagg gcaaccaatc gatgcatgct gcgagttata ccatttctaa tgccacaaga
     8341 atttcagcac gcacctcgtt atctgcttcg ctttgcaagg cagactgtag ccccaggcgg
     8401 gcttctttgc cgccgatttg ccccaaggcc caggcggcgt ggatgcgcac caggggttct
     8461 gcgtcgcctt ccagggcttg gattaggggg gagatggcgt cagtatgatg tgtgttgccc
     8521 agagcgaccg ctacattgcg caggtagccg cgtcgtttgg cgcgttttac cgggttatcc
     8581 ttaaactttc ggttgaactc ctgggggttg agagccagct cggggatcaa g
//
LOCUS       DBNGSX010000580         2838 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1099934, whole genome shotgun sequence.
ACCESSION   DBNGSX010000580 DBNGSX010000000
VERSION     DBNGSX010000580.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1099934"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="AC16CB_16895"
     CDS             complement(<1..1103)
                     /locus_tag="AC16CB_16895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344203.1"
                     /translation="MKKTHVAYVPGPETSPVEEEFINSLLGQFRALGHYVQDVPDPHT
                     DILITTAPFGQPISWRKALLFTGRARYKYHHSPMVFTLIKVTPEVLDTQLAHFRQALE
                     KEEPDPKDYLFPGLASSAYKVLHEQASRGGAILALERLLQAQSKCINILLLVGDQDIE
                     AAYHFDLVGSHPLTIARDNPNSLKEIVLRMVTRVSAHEVTYHQVVGDPIPGELWNAAK
                     TPAWMLAAARNLDERQFFTDTVRISALVDVPAVGDAVASQYSEGCFATWDPDLNALLA
                     TVTGSARPVSKGSITEDDLAVIVGAKPDGSGALVRLVEGKRNDPPSSEAVEMIGMDSL
                     LPKIVLNFDGRVGAEVPVIRSKLHGHRGISSFNP"
     gene            complement(1118..2122)
                     /gene="add"
                     /locus_tag="AC16CB_16900"
     CDS             complement(1118..2122)
                     /gene="add"
                     /locus_tag="AC16CB_16900"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010635442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HIR3344204.1"
                     /translation="MASFIDPSLPWIDLHRHLDGSLRLETILDLGRKHNLPLPAWDVE
                     NLRPHVQITEPQPGVMAFIAKFKWMVGVLVDYAAVRRIAYENVEDAQKEGLDYVELRF
                     SPWFMAEPHRLRPAGVVEAVVDGVQAGIRDTGMRVKLIGILSRTYGPEIAWKELEALL
                     TQREQLVALDLAGDEANFQGELFNKHIRRGRDAGWNITVHAGEAAGPHSVWHAVRQMG
                     AARLGHAVHAAEDPSLLDYLAEQRIGIEANLTSNVQTSTVPDYPSHPLRRFLEHGILA
                     TINTDDPGISNITLAYEYDVAAPAAGLTPSQIRQAQRNALEVAFLTQEEKESLLAKGS
                     "
     gene            2237..2464
                     /locus_tag="AC16CB_16905"
     CDS             2237..2464
                     /locus_tag="AC16CB_16905"
                     /inference="COORDINATES: protein motif:HMM:NF024143.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HIR3344205.1"
                     /translation="MRRFMAFMMGAIMGGLVGATLALLLAPSSGTELRTQMQERVEGL
                     REEILAAAAERRTELEEQLAALRAPQKSGEA"
     gene            complement(2482..>2838)
                     /locus_tag="AC16CB_16910"
     CDS             complement(2482..>2838)
                     /locus_tag="AC16CB_16910"
                     /inference="COORDINATES: protein motif:HMM:NF044618.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344206.1"
                     /translation="LSQFAGKKVQIRFEYITDAAVNGEGFLLDDVSIPEIDYATDFET
                     DDGGWTAEGFVRIQNTLPQEFRLALIKQGRATEVEYISLTADNTAEIPLQFGDGFNEA
                     VLVVAGTTRFTRQSAS"
BASE COUNT          618 a          763 c          810 g          647 t
ORIGIN      
        1 gggttaaacg atgagatgcc ccgatgtcca tgaagtttcg agcgaatcac cggcacctca
       61 gcccccactc tgccgtcgaa attcagcaca attttgggta agaggctgtc catgccaatc
      121 atttcaactg cctctgagga aggcggatcg ttgcgtttcc cctccacaag gcgcaccagt
      181 gcgccggatc catccggctt agccccgaca atgaccgcta ggtcatcttc cgtgatgctg
      241 cccttggata ccggcctggc gctaccggtg actgtggcaa gcagcgcatt gagatctggg
      301 tcccaggtgg caaaacaacc ctcgctatat tgggatgcta cagcgtcgcc gacggctggc
      361 acatcgacaa gcgcggagat tcgcacggta tcggtaaaga attggcgctc atctaaattc
      421 ctggctgctg caagcatcca agcgggtgtc tttgctgcat tccataattc tccagggatt
      481 ggatcaccaa caacctggtg atatgtcact tcatgggcgc ttacccgggt aaccattctc
      541 aaaacaattt ccttcaagga gtttgggtta tcccttgcga tggtcagcgg atgtgagccg
      601 accagatcaa aatggtacgc cgcctcgatg tcctgatcgc ccactaataa tagaatgttg
      661 atgcattttg attgcgcctg aagcaatctc tccaacgcta agatcgcccc gcctctgcta
      721 gcctgctcgt gaagcacctt gtatgcgctg gaggccagac caggaaagag ataatctttt
      781 ggatctggct cttccttttc caatgcctga cgaaagtgcg ccagttgcgt gtccagcact
      841 tccggagtga ctttgatcag cgtgaaaacc atcggagagt gatggtactt ataacgagcc
      901 cgtccggtaa acagtaaggc ttttcgccag gagattggct gaccgaatgg agcggttgtg
      961 atcaggatat ctgtatgcgg gtctggcaca tcctgcacat aatgcccaag ggctcgaaac
     1021 tgtcccagca gggagttgat gaactcctct tcgacgggag aagtctcagg ccctgggaca
     1081 taagcgacgt gagttttctt caaccaacta tgcatcgtta agaacctttc gcaagcagtg
     1141 attctttttc ctcctgtgtc aggaatgcca cttccagagc gttgcgttgc gcctggcgaa
     1201 tttgactggg ggtcaggccg gcagcggggg cagccacgtc gtactcatat gccagtgtga
     1261 tgttgctgat cccagggtca tcagtgttga tggtcgccag gatgccatgc tccaggaagc
     1321 ggcgcagagg atggctgggg tagtcgggga cagtgctcgt ctgaacattg ctggtcaggt
     1381 tagcttcgat tccaatccgt tgttccgcca ggtaatccag caaggatggg tcctcggcgg
     1441 catgcacggc gtgacccagg cgcgctgcgc ccatctggcg cactgcatgc cagacgctat
     1501 gcggcccggc cgcttctccg gcatggacgg tgatattcca gccggcatcg cgcccgcggc
     1561 ggatatgctt gttgaataat tcgccttgaa aattcgcctc atcgccggcc aggtccaggg
     1621 ctaccagctg ctcgcgttgg gtgagcaggg cttccagctc tttccaggcg atctccgggc
     1681 cataggtgcg gctgaggatg ccgattagct taacccgcat gcccgtgtcg cgaataccag
     1741 cctggactcc atccacaacc gcctcgacca caccggcggg ccgcaggcgg tggggctctg
     1801 ccataaacca ggggctgaag cgtagctcaa cataatccag cccctctttc tgggcatctt
     1861 ccacattttc atatgcaatg cggcgcactg cagcgtaatc gacgagcacc ccgaccatcc
     1921 acttgaactt ggcgataaaa gccatcacac cgggctgcgg ttcggtgatt tgcacgtggg
     1981 gtcgcaggtt ttcgacatcc catgcgggta ggggcaggtt gtgcttgcgc cccaggtcca
     2041 gaatggtctc caggcgcaag cttccatcca ggtgtcggtg tagatcaatc caagggagag
     2101 aggggtcgat aaatgaagcc atggcccagg ctaggtcggt tagtgaagct ttatttcagg
     2161 atacttgatg taatagcggg gtaagttctt gttataatat cctgaaatag aaaaatttac
     2221 aattgaggag gacagtatga ggcggtttat ggcctttatg atgggggcaa tcatgggtgg
     2281 gttggtaggt gcgacgctag cactactgct agctccctcc tccggcacag aactccgcac
     2341 gcaaatgcaa gaacgcgtgg aggggcttcg cgaggagatt cttgcagcgg cagccgaacg
     2401 gcgaactgaa ctcgaggagc aactggcggc tcttagggcg ccccagaagt caggcgaagc
     2461 ctaaggctcg aaataaatct gttaggaagc tgactggcga gtgaagcggg ttgtcccagc
     2521 tacaaccagc acggcttcat taaatccatc tccaaactgg agggggatct cggcggtgtt
     2581 atcggcggtg agagagatgt actcgacctc ggtagcgcgc ccttgtttga tcagcgccag
     2641 gcggaattcc tgcggcaatg tgttttggat gcgcacgaaa ccttctgccg tccaaccccc
     2701 atcatcggtt tcaaaatcgg tagcgtagtc aatctctggg attgagacat cgtcaagtaa
     2761 gaagccttcg ccgttgaccg cggcatcggt gatatattcg aagcgtatct gcactttctt
     2821 gcctgcgaat tgagagag
//
LOCUS       DBNGSX010000581         5054 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1100075, whole genome shotgun sequence.
ACCESSION   DBNGSX010000581 DBNGSX010000000
VERSION     DBNGSX010000581.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5054)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5054
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1100075"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2119
                     /gene="uvrA"
                     /locus_tag="AC16CB_16915"
     CDS             <1..2119
                     /gene="uvrA"
                     /locus_tag="AC16CB_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR3344207.1"
                     /translation="HFSEHLACPEHGSVLPEIEPRTFSFNTPHGACPDCQGLGGKLEI
                     DPDLLIPDPHLSLSEGAIAISEWSGPREEGGYYWQTLEAAAREFHIDLDAPVKTVSPE
                     KLDIVLYGTRGREVTVHYQNRDGRRATFSTPFEGVIGNLQRRYNETSSEYMREKISEF
                     MSERTCPTCKGARLRTEALAVTLDGVNIIEVTTWPVLRTLEWVHRLSAEDTPLNTRQQ
                     VIAERILKEIESRLGFLVNVGLDYLTLKRSAASLSGGEAQRIRLATQIGSRLMGVLYV
                     LDEPSIGLHPRDNSRLLTTLKNLRDLGNTVLVVEHDEETIRSSDWVLDLGPGAGEHGG
                     KVVCEGTVETILSHPESLTGAYLSGRMRVPIPDKRRTGNGKTLVVVGARENNLKNIDV
                     RIPLGRLVCITGVSGSGKSTLMVEILYKALARHLHGARAQPGDHDRVEGLEHIDKIIN
                     IDQSPIGRTPRSNPGTYTGLFDAIRALFAELPESKIRGYKPGRFSFNVHGGRCEACQG
                     QGQLRIEMQFLPDVYVPCDVCHGARFNRETLQVRFKEHNIADVLDMTVDHALEIFEAF
                     PGMVNKLKTLQEVGLGYIRIGQPAPTLSGGEAQRVKLSRELSRRATGRTMYVLDEPSV
                     GLHAADVHKLIEVMQRLVDAGNSVLIIEHNLDTIKVADYLIDLGPEGGDGGGLVVAEG
                     TPEQVCERDDSYTGQYLRPFICK"
     gene            complement(2183..2293)
                     /locus_tag="AC16CB_16920"
                     /pseudo
     CDS             complement(2183..2293)
                     /locus_tag="AC16CB_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002766081.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            complement(2314..3609)
                     /locus_tag="AC16CB_16925"
     CDS             complement(2314..3609)
                     /locus_tag="AC16CB_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3344208.1"
                     /translation="MPPSSWDKSRLQAVAQRVDQPLDAHRQLSTQGTFAALKHPNYRL
                     WFAGQLVSLVGSWMQTTAQGYLIFELTQSPAYLGYVGFAAGIPSWLFTLYGGLIADRM
                     PRRNLLVMTQSAMMVLAFGLATLTFAGIVQPGHILGFAFCLGIANAFDAPARQSFVLE
                     MVSREDLTNAIALNSSMFTAATVVGPAVGGLLYAALGPAWCFTINGASFMAVITALLL
                     MRLKPASHTPRHGSALADVAAGIGYVRKNVIPRTVITHLAMVSMFGISFVSLMPAWAV
                     RVLGGDATTNGFLQSARGVGALSGALMIAAFGGYIVRGRLLTLGGFILPLTLLAFSAV
                     RWLPLSLLTLVGVGWGFMVFVNSSNALLQSHVPDDLRGRVMSIYTLAFFGLMPVGALL
                     AGGAATWVGEPLTVALAASMLLVFGALVWIKVPQLRSSD"
     gene            3787..4302
                     /locus_tag="AC16CB_16930"
     CDS             3787..4302
                     /locus_tag="AC16CB_16930"
                     /inference="COORDINATES: protein motif:HMM:NF012976.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase family protein"
                     /protein_id="HIR3344209.1"
                     /translation="MMIRLKLRSIAVMILVALYLSACTTETTQVSITPTTSQENRNQV
                     AQTCPPTRSDAEGPFYKPDAPLRDKVGEGYVLQGMVLSSAGCVPIPGAQLEFWLAGSD
                     GEYSDDYRATLLADDQGAYRFESNLPPPYSGRPPHIHIRISAEGYQTLVTQHYPNEGE
                     TSANMDIVLIP"
     gene            complement(4308..4643)
                     /locus_tag="AC16CB_16935"
     CDS             complement(4308..4643)
                     /locus_tag="AC16CB_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344210.1"
                     /translation="MPIGWGEGLDQAARYLDQMSNAKDLHVISWYATGPFSYFFKGHT
                     RFMGAESGDEDEDRNWERFVTADYAVVYVSQWQRNIPKKVLGHLSEQSPEYSVWINGI
                     EYARVYKVR"
     gene            complement(4788..>5054)
                     /locus_tag="AC16CB_16940"
     CDS             complement(4788..>5054)
                     /locus_tag="AC16CB_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344211.1"
                     /translation="LTYLWRSTPVVLLGLILAAWGLVRRLKPFDRRDTRSLVYVLLLF
                     VFVFSLMMTLGAKKSDRYLVPVFAPLDICAGLGWVSLAIWIRAR"
BASE COUNT         1263 a         1441 c         1348 g         1002 t
ORIGIN      
        1 gcacttctcc gagcacctgg cctgcccgga acacggctcg gttttaccag agatcgagcc
       61 gcgcaccttc tccttcaata ctccccacgg tgcctgtcct gactgccagg ggctgggtgg
      121 caagctggag atcgatcccg acctgcttat ccccgacccg cacctttcct tgagtgaagg
      181 tgccatcgct atctctgaat ggagcggccc gcgcgaagaa ggcggttact actggcaaac
      241 cctggaggct gcagcccgag aatttcacat tgacttagat gccccagtga aaacggtctc
      301 gcccgaaaag ttggacatcg tgctgtatgg tacgcgcggc agagaagtca ccgtgcacta
      361 tcagaaccgt gacgggcgga gggcaacctt cagcaccccc ttcgagggcg tgatcggcaa
      421 tctacagcgt cgttacaacg agaccagctc cgaatatatg cgtgagaaga tcagcgagtt
      481 catgagcgag cgcacctgcc ccacttgtaa gggggccagg ctgcgcacag aagcgctggc
      541 ggttacgctt gacggtgtca atatcatcga agtgacaacc tggccggtgt tacgcaccct
      601 agagtgggtg caccgtctga gcgcagagga cacgccgctc aatacccgcc agcaggtcat
      661 cgctgaacgc atattgaagg agatcgaatc ccggctggga ttcctggtga atgtcggcct
      721 ggactacctt accctgaaac gctcggcagc ctccctttca ggaggcgagg cgcagcgcat
      781 ccgcctagct acccagatcg gttcacgcct gatgggtgtg ctgtatgtgc tggacgaacc
      841 ctctattggc ctgcacccgc gggataactc ccgcctgtta actacgctaa aaaatctgcg
      901 tgatctgggg aataccgtac tggtggtgga acacgatgaa gagactatcc gctcatcaga
      961 ctgggtactg gatctggggc ctggggctgg cgaacatggc gggaaggtgg tctgtgaggg
     1021 aacagtagag accatcctga gccacccaga atcgctcact ggcgcctacc tctccgggcg
     1081 gatgcgcgta cccatccccg ataaacggcg caccggcaat ggcaagacgc tggtggtagt
     1141 tggggcgcgc gagaacaacc taaaaaacat cgatgtgcgt atcccgctgg gcagactggt
     1201 gtgcatcaca ggcgtctcag gctctggcaa atccaccctg atggtggaaa tcctgtacaa
     1261 agccctggcg cggcatttac atggggcgcg tgcgcaaccc ggcgaccacg accgcgttga
     1321 gggtctggaa cacatcgaca agatcatcaa catcgaccag tcgcccatcg gtcgcacgcc
     1381 gcgctccaac ccgggcacgt ataccggctt gttcgacgcc atccgcgccc tattcgccga
     1441 gctacctgag agcaagatac gcggctacaa gcccgggcgc ttttccttca atgtgcatgg
     1501 cgggcgctgt gaggcctgtc aggggcaagg tcagctgcgc atcgagatgc agttcctgcc
     1561 cgatgtatat gtaccctgtg acgtgtgtca tggagcacgc ttcaaccgcg agaccttgca
     1621 ggtgcgcttc aaagaacaca atattgccga cgtgctggat atgactgttg accatgccct
     1681 ggaaatcttc gaagccttcc ctggaatggt gaacaagctc aaaaccctgc aagaagtggg
     1741 cttaggatat atccgcattg ggcagccagc ccccacgctt tcaggaggcg aggcgcagcg
     1801 cgttaagctg tctcgagagc tttcacgccg cgctaccggg cgcaccatgt acgtactgga
     1861 tgagccttcg gttggcctgc atgctgctga tgtgcacaag ctgattgaag tgatgcagcg
     1921 tttagtcgat gctggcaaca gcgtgttgat catcgagcat aatctcgaca cgatcaaagt
     1981 cgctgattac ttgatcgacc ttggacccga aggtggtgat ggcggcggat tggtggttgc
     2041 agagggcacg ccagagcaag tgtgcgagcg ggatgattcg tacaccggtc aatatctgag
     2101 accgtttatt tgtaagtgat agggagaagg acacgatacg gtatcgggcg cacacagccg
     2161 cgatagcaat attttagcca tttttggact cttctttaga ggcgccccag ccccaatctt
     2221 caccaaaagt catggtgcct aaagccagtt cagatactct cagtccgctt ttacccaata
     2281 gcttatatcc cattctcttc tcctcaatat caattaatct gaactacgca gttggggtac
     2341 cttgatccaa accaaagcgc cgaaaaccaa cagcatcgag gcagccaaag ctaccgtgag
     2401 cggctctcca acccaggtgg cagcgccgcc agctagcagg gcgccaacag gcatcaatcc
     2461 aaagaatgcc agggtgtata tggacatcac ccggccgcgc agatcgtctg gcacgtggct
     2521 ttgcaacagg gcattggatg agttgacaaa caccatgaac ccccagccaa cgccaaccag
     2581 tgtcaacaag gatagcggaa gccagcgcac cgcggagaac gccaacaagg tgagcggcaa
     2641 gatgaagccg cccagggtaa gcaatctccc gcgcacaata tagccaccaa atgccgcgat
     2701 catcaatgcc ccgctcagcg ctcccacacc tcgcgccgat tggaggaaac cgttggtggt
     2761 ggcatcacca cccagcactc tcaccgccca ggcgggcatc aaggaaacaa agctgatccc
     2821 aaacatgctg accatagcca agtgagtgat caccgttcgg gggattacat tcttgcggac
     2881 atagccgatg cccgcggcca catctgccaa ggccgagcca tgtcgtgggg tgtgtgaagc
     2941 tggttttaac cgcatgagta ataaggcagt aatcactgcc ataaaagaag ctccattgat
     3001 ggtgaagcac caggctggac caagggcagc atacagcagc ccgcccactg ccggcccaac
     3061 cacggttgca gcggtgaaca tgcttgaatt taaggcgatg gcattggtca gatcttcccg
     3121 cgagaccatc tccaacacaa aagattgcct ggctggcgca tcaaaggcgt tagcgattcc
     3181 caagcagaaa gcaaaaccca ggatatgccc cggctgcacg atgccagcga aggtcagcgt
     3241 ggccaaacca aacgccagaa ccatcatcgc tgactgggtc atcaccagca gattgcggcg
     3301 gggcatacga tcagcaatca agccgccgta caaggtaaac agccaggagg ggatccccgc
     3361 agcgaacccc acatagccca aataggctgg tgattgggtc agctcaaaga ttaagtaacc
     3421 ttgcgcagta gtctgcatcc agctgccgac cagcgaaacc agttgaccag cgaaccacag
     3481 gcgatagtta ggatgcttga gcgcagcaaa ggtcccctgg gtgctcagtt gtctgtgggc
     3541 atccaaaggt tggtcaaccc gttgggcaac cgcttgcaat ctacttttat cccaggatga
     3601 aggaggcatg cgcccattat aaacctcatg attattcgaa ttatggaatt cagaatgtca
     3661 aatgccagcg cagcgaacct gttgccagcc tacgttataa tctttccaat acctacgcaa
     3721 ttgataaaga attcctcagt tgcgtaactt ttgacggcaa tattagatac aaatcaggag
     3781 tgcgtgatga tgatccgctt aaagttacga tcaatagcag taatgatact ggtagcactg
     3841 tatttatctg cctgtacaac cgagaccacc caggtttcca tcacaccaac tacttcgcag
     3901 gaaaaccgaa atcaggttgc ccaaacctgt ccgcccaccc ggtctgatgc cgaggggcca
     3961 ttctataagc cagacgcgcc attgcgcgac aaagtgggag aaggctatgt actgcaaggc
     4021 atggtgctct cttcggcggg ttgtgttcct atacctgggg cacagctaga gttctggctg
     4081 gctgggtccg atggcgaata cagcgacgac tatcgcgcca cattgctggc ggatgaccaa
     4141 ggcgcctatc gttttgaaag caacctgccc ccaccctact cggggaggcc accgcacatt
     4201 catatccgca tcagtgcgga aggataccaa acgctcgtca cgcagcatta ccccaatgag
     4261 ggggagacta gcgccaacat ggatattgtg cttataccct aatcttttta gcgcacttta
     4321 tacacacggg catactcgat gccgttgatc catacggaat actctggaga ctgctcagag
     4381 agatggccaa gcaccttttt gggtatgttc ctttgccact ggctgacata gacaaccgca
     4441 taatccgcgg tgacgaaacg ttcccagttg cgatcctcgt cctcatcacc cgattccgcc
     4501 cccataaagc gcgtatggcc cttaaagaaa tacgagaatg gacccgtggc ataccaggaa
     4561 atcacgtgta aatccttcgc attcgacatt tgatcgagat atcgcgctgc ctgatccaac
     4621 ccctctcccc aaccgattgg cattatctca atagctttgc ttccgcctcc catccacggg
     4681 ttatagtatg acgcataata ggggaaagta ttcaaggaca gggccatttg ggtgccaacg
     4741 atgaagcata ggagcaggct ggtggcatac cgggtgtaac cggcggccta tcgcgcgcga
     4801 atccagattg ccagagaaac ccacccaagc ccagcgcaaa tatccagcgg agcaaataca
     4861 gggacaaggt agcgatcaga tttcttcgcg cccagggtca tcatgagtga aaaaacaaaa
     4921 acgaataata acaggacata taccaaggac cgcgtatcac gccggtcaaa gggcttcaat
     4981 ctcctcacca atccccatgc agcaagtatt aaaccgagaa gaacaaccgg tgtactccgc
     5041 caaaggtaag tgag
//
LOCUS       DBNGSX010000582         2872 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_199285, whole genome shotgun sequence.
ACCESSION   DBNGSX010000582 DBNGSX010000000
VERSION     DBNGSX010000582.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_199285"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="AC16CB_16945"
     CDS             complement(<1..605)
                     /locus_tag="AC16CB_16945"
                     /inference="COORDINATES: protein motif:HMM:NF040248.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3344212.1"
                     /translation="MTRYIIRRLLQAFPLLFLISLVLFVLMQNMGDPLATMGGRKATR
                     SEDRIRLARQLGLDKPVAVQYLYWLVGNDWAKTDMDGDGVAETPGKRKGVLRGDWGIS
                     LVNRQPVTKVIAQRLSNTLLLMLTAEALIIVFALIIGIYSALNQYSALDNAITTTTFV
                     FYSMPVFFFALLAMYIFAVNFKRWGLPYLPTVGMFEPAAGQT"
     gene            complement(670..2433)
                     /locus_tag="AC16CB_16950"
     CDS             complement(670..2433)
                     /locus_tag="AC16CB_16950"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3344213.1"
                     /translation="MLNKQRLLTWFSLLIIASMLLAACGGPATQPPAEEPPAEQPPAE
                     EPPAEQPPAEAPPTEAPPEPPAERKVVTFIWTQEFDTLNPLYTQMWFVSVLFPVYACQ
                     AWWFDDQNAPVPNLVTEIPSGENGGISEDGRTITLKLRDDIVWSDGTPITSADFKFTY
                     DMELADSNAVASRSPYDLLESLETPDERTVVATFTEPYAPWLASLFSGIAGISIIPAH
                     ILQPVFDAEGTIDTAEWNLFPSVGCGPFVLEEWESGSFARFVANDNFWLGRPKLDELF
                     FRFVPDDASMIAALVAGDGDVGTFFAYGDLPQLEAAGIKILNSFSGYNEGWYFNLHPE
                     KGNPALKDVKVRQAIALAFNREKLVQDLLLGRTGVAATDWDNSPWVDPSIEPWPYDPE
                     RAKALLEEAGWTDTNGDGTVDKDGVELVLKYGTTNREVRQDTQAVAQQDLAAVGIGTD
                     LQSYDSDLFFSSYAEDGPSANFSLDIIEYSDVPDFPDPNASVWRCAEVPSDESPAGVN
                     TSGLCDEELEALFVQQETQVDFAERQQTFYQITKYIYDNVLWLGLWEDPDLFGFSDRM
                     LNVKMSGATSFFNVFEWDIAQ"
     gene            complement(2669..>2872)
                     /locus_tag="AC16CB_16955"
     CDS             complement(2669..>2872)
                     /locus_tag="AC16CB_16955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344214.1"
                     /translation="KKLKIFTVTRYALPERTAKLVKGQSVGPVKALLDNCARARPEVW
                     KTLESGIKKAISARTESSDSDFD"
BASE COUNT          598 a          789 c          847 g          638 t
ORIGIN      
        1 gtctgcccgg ctgccggctc gaacatgccc acagtcggta agtagggcaa cccccatctc
       61 ttgaagttga ccgcgaagat gtacatcgcc agcagggcga agaaaaatac cggcatggag
      121 taaaaaacga aggtcgtggt cgtgatggcg ttatccagcg ctgaatactg gtttaacgcc
      181 gagtagatgc cgatgatcaa ggcgaaaacg atgatcaatg cttcggcggt cagcatcaag
      241 agcaaggtgt tcgagaggcg ctgggcgatc actttggtga ccggttgccg gttgaccagc
      301 gagatgcccc aatcgccgcg cagcacgcct ttgcgcttgc ccggcgtctc cgccaccccg
      361 tcgccgtcca tatcggtctt ggcccagtca ttaccgacca gccagtaaag gtattggaca
      421 gctactggtt tatccagccc gagctggcgc gccaggcgga tgcggtcctc gctgcgcgtg
      481 gccttgcgcc cgcccatggt cgccagcggg tcacccatat tttgcatcaa gacgaacagc
      541 accaggctga tcaggaaaag cagcgggaag gcttgcagca acctgcggat gatatagcgg
      601 gtcatgcgta aatccttctt ggcagcgtgc aggtgacatt agccacctgc acgccaatcc
      661 gaacattgat tattgggcta tgtcccactc aaatacgttg aagaatgagg tggcgccgga
      721 catcttgacg ttcagcatgc gatcgctgaa gccgaacagg tccgggtcct cccacaaacc
      781 caaccagagc acgttgtcgt aaatatactt ggtgatctgg tagaaagtct gctggcgctc
      841 tgcaaagtcg acctgggttt cttgctggac gaacaacgcc tccaactctt catcgcacag
      901 gccgcttgtg tttaccccgg cagggctctc gtccgagggc acttctgcgc accgccagac
      961 acttgcgttg ggatcgggga agtctggtac atcggagtac tcgatgatgt ccagcgagaa
     1021 gttagccgaa ggcccgtcct cggcgtagct ggagaagaag agatccgagt cataactctg
     1081 caggtctgtg ccgatgccca ccgccgccag gtcctgctgg gccactgcct gggtgtcctg
     1141 gcgcacctcg cggttggtgg tgccgtactt cagcaccagc tcgacgccgt ctttgtcaac
     1201 cgtgccgtcg ccatttgtat ccgtccagcc ggcttcttct agcagcgcct tggcgcgctc
     1261 ggggtcgtag ggccagggct cgatgctcgg gtctacccag ggggagttat cccaatccgt
     1321 ggcggcgacg ccggtgcgcc cgagaagcag gtcttgcacc agcttctcac ggttgaaggc
     1381 cagggcgatc gcctggcgca ctttcacatc cttgagcgcc gggttgccct tttctgggtg
     1441 cagattgaag taccagcctt cgttgtagcc tgagaaggag ttgaggatct tgataccggc
     1501 tgcctcgagc tggggcaggt cgccgtaagc gaagaacgtg cctacatcgc cgtcacctgc
     1561 caccagggcg gcgatcatgg aggcgtcgtc cggcacgaag cggaagaaga gctcatccag
     1621 cttggggcgg cccagccaga agttgtcgtt ggctacgaag cgggcgaagc tgcccgattc
     1681 ccattcttcc agaacaaacg gaccacatcc aacactgggg aataaattcc attcagcagt
     1741 atcaatggta ccctcggcat cgaagactgg ctgaaggata tgagccggga taatgctgat
     1801 gccagcaatc ccgctgaata gtgaagccag ccatggggca tatggctccg tgaaggttgc
     1861 aaccacagtg cgctcatcgg gggtctcgag cgactccagc aggtcgtatg gggagcgcga
     1921 tgccaccgcg ttcgagtcgg caagttccat atcataggtg aacttgaagt ccgccgaggt
     1981 gatcggcgtg ccgtctgacc acacgatatc gtcgcgcagc ttcagggtga tcgttctgcc
     2041 gtcttcggat atcccaccgt tctcacccga gggtatctca gttaccaggt ttggaacggg
     2101 ggcgttctgg tcatcgaacc accaggcctg gcacgcatag accggaaaga gcacggagac
     2161 aaaccacatt tgggtgtaca gcgggttgag cgtgtcgaac tcttgcgtcc agatgaaggt
     2221 caccactttg cgctcagcag gtggctcagg gggtgcttca gtaggtggcg cttctgcggg
     2281 tggttgttct gcaggtggtt cttctgcagg tggttgttct gcaggtggct cttctgcggg
     2341 cggctgggtg gcgggtccgc cgcaggcagc cagcagcatg ctggcgatga tcagcagcga
     2401 aaaccaggtc agtaggcgtt gtttattcaa catagtattc tcctcctaaa aatttagaat
     2461 ggactgactt tgatgggcgg gaatttacct tccacagttc atcgatggat aatatgcttt
     2521 tcaaccacct ccttactctg ctaaaagtct ggtttttaat caatgctggg tgttcgtccc
     2581 tgtataccag gcatgagggg cgagacaaat ctttactatt atatatggaa tacgcccaag
     2641 tatataggaa agatcaagga attttaattt agtcaaaatc gctgtcgctc gactcggtgc
     2701 gggcgctgat ggccttctta atccctgatt cgagtgtttt ccaaacttca ggtcgggcac
     2761 gtgcgcaatt atccagcagt gcctttaccg gtccaacaga ttgccctttg accagcttgg
     2821 ctgtccgctc tggcagagcg tatcgggtga cggtgaagat cttgagcttc tt
//
LOCUS       DBNGSX010000583         3450 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_962071, whole genome shotgun sequence.
ACCESSION   DBNGSX010000583 DBNGSX010000000
VERSION     DBNGSX010000583.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_962071"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..319)
                     /locus_tag="AC16CB_16960"
     CDS             complement(173..319)
                     /locus_tag="AC16CB_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HIR3344215.1"
                     /translation="MLFAPKEKGQGLVEYALILVLIAVVVIVILALLGPAVGNVFSNI
                     VRNL"
     gene            complement(446..1501)
                     /locus_tag="AC16CB_16965"
     CDS             complement(446..1501)
                     /locus_tag="AC16CB_16965"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3344216.1"
                     /translation="MVAPETQEENVWEELLHQVQSELEQTKKQLEEISLLSEQSQIEV
                     GKLTQRNAAITSNLQRIQAQFDNLPRGDIRMAYDTALDAQQRLFVMRGQAEKLQADQH
                     HTKRFLELLERVQKFLEGVNADDAPKRGGQYMIETVEMMIEAQEAERLRLSRQMHDGP
                     AQALSNFILQTEIALRLFDLDQQQAREELGNLKTSATGAFQKVRDFIFELRPMMLDDL
                     GLSPTIKRYVDALKGQHGTEILLTVTGVERRLESYIEVMVFRAIQELLNNALRHSQAT
                     QINVQLDIGETNVKVAVEDNGKGFDVEILEERGRVGIKLIKERAEKLGGYLDVDSAAG
                     RGTRISFQVPAPGIDPI"
     gene            complement(1507..2208)
                     /locus_tag="AC16CB_16970"
     CDS             complement(1507..2208)
                     /locus_tag="AC16CB_16970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3344217.1"
                     /translation="MFEISIIIVDDHPLFRQGVADVLSLEPDLRVVAQSSSGEEGLEL
                     ILEHQPMVAIVDVNLPGLNGQQLARKVVAERIPTRIMLLTAYDDTEQKIHAMRVGAAA
                     YCTKDVVPENLVKRIRLVASGKYVIGDDVYDSASLQRWLSIQTEGAVGLYSDAGESFQ
                     PLSSREMEVLTQVTKGMSNKEVAVLLGISQQTVKNHVTSILRKLGVEDRTQAAIFAIK
                     RGWVRVHQANIESQE"
     gene            complement(2287..>3450)
                     /locus_tag="AC16CB_16975"
     CDS             complement(2287..>3450)
                     /locus_tag="AC16CB_16975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344218.1"
                     /translation="LWMFSKLQPASNRMMALLVGALLVGEWHLVWAAVSGMETALFSL
                     IVLAIFSMLIAHNQNWIILGGFIGLSIWIRPDGLTLLAPALLVCIMQASSNYQRVSRI
                     MLLAVGFSLLFSPYLLWNRMLSGVIWPNTFYAKQAEYAVELLEPLGKRLLEQAALPNI
                     GIGFMLIPGFIYFFYRSFRHHNWGVVAGSFWIIGYLVVYALRLPVTYQHGRYVMPMMP
                     PYFLWGLAGTTSLFLWNSPILWRRVLSKAWLVSIGLLCITFWYLGAQAYGRDVAFIES
                     EMVATSRWIEEYTEPDVLIGAHDIGALGYFGGRRILDMAGLVSPEVIPFIRDEAQIDN
                     WLTKENADYLVTFPSWYSELTQGLESVYQTGGKYSQAFDGENMVVYRWRLRIR"
BASE COUNT          981 a          862 c          641 g          966 t
ORIGIN      
        1 tacattgtgt agatgatcca atttgtatct taaacgttct atgtaaaaag tcaagacaat
       61 ttcggtactt tagtcctatc ctatacaaaa ctgtaaagaa atctgccatt aagtgttaag
      121 gctctgcttg tgcagagcct tagacttgga aaaatggata tgaacttgag ggttataaat
      181 ttcttacaat gttgctgaag acatttccaa cggctgggcc taaaagagca agaatgacga
      241 ttacaacaac cgcaatcaaa acaagaatta gcgcatactc tactaagcct tggccttttt
      301 ccttgggagc aaataacatg actcatacct cctttctctt aggattccac acgaacttta
      361 taagataatc tcattataaa aaatctggat gaattttgca ataggttttt cctgaaaaat
      421 tccttgcaat cctgataagt aggtgttaaa ttgggtcaat tccaggggca gggacttgaa
      481 aagatatccg agttcctctg cctgcggcgc tatccacatc taaataccct cctaattttt
      541 ccgcgcgttc tttgatcagc ttaatgccca ctcttccccg ctcttctaag atctcgacat
      601 cgaatccttt gccattatct tcaacagcta ctttcacatt ggtttcccct atatccagct
      661 gtacatttat ttgggtagct tgactatgac gaagggcatt gttgagaagc tcttgaattg
      721 cccgaaaaac catcacttca atataagatt ctaaacgacg ctcaactccg gtcacggtta
      781 gaagaatttc tgtaccgtgt tgccccttca atgcatcgac atatcgtttt attgttggag
      841 ataatccaag atcatcaagc atcatgggtc gtaattcaaa aataaaatcc cgcactttct
      901 gaaacgcacc cgttgccgat gtctttaggt tacccaactc ttcacgagcc tgttgctgat
      961 ctaaatcgaa tagacgcagt gcaatttcag tctgcaggat aaagttagaa agcgcctgtg
     1021 caggaccatc atgcatctgg cgagacaatc gtaaccgttc agcttcctga gcttcaatca
     1081 tcatctccac agtttcgatc atatattggc cgcctctttt aggtgcatca tcggcattga
     1141 caccttcaag gaatttctga acccgctcta ataattcaag aaacctcttg gtatgatgct
     1201 gatcagcctg caacttttct gcctggcctc gcatgacaaa caatcgttgt tgggcatcta
     1261 atgccgtatc atatgccata cgtatatcgc cgcgaggcaa attatcaaat tgtgcttgta
     1321 tcctttggag attagatgta attgcagcat tgcgctgggt tagtttgcct acctcgatct
     1381 gactttgttc agacagtaag gagatctctt ccagttgttt tttcgtttgt tctaattcgc
     1441 tctgaacctg gtgcaataat tcttcccaca cattttcttc ctgagtctct ggtgcaacca
     1501 taaatatcac tcctgagatt caatattggc ttggtgcaca cgtacccaac cacgcttgat
     1561 tgcaaatatc gctgcctgtg tgcgatcctc tacaccgagc tttcgtaaaa tggaagtaac
     1621 gtggtttttt accgtttgtt ggctaatacc caataataca gcaacttcct tattactcat
     1681 tccttttgta acctgagtta acacttccat ttcacgcgat gataggggtt ggaaagattc
     1741 gcctgcatca ctatacaagc ctacggctcc ttctgtctgg atagagagcc atcgttgcaa
     1801 acttgcacta tcataaacat catcacctat tacatatttt cctgatgcaa ctaatcgaat
     1861 tctttttacc agattttcag gaacgacatc ttttgtgcaa tacgccgctg cacctacacg
     1921 catggcgtgg attttctgct ccgtgtcatc ataagctgtc agaagcatga tacgagtggg
     1981 gatcctttcc gcaaccactt ttcgggcaag ctgttgtccg tttaaacccg gaaggtttac
     2041 atctacaata gccaccatag gctgatgttc aagtatcaac tcgagtcctt cttctccact
     2101 cgaagactga gccacaaccc taagatctgg ctctaaggag agtacgtctg caaccccttg
     2161 acgaaagaga ggatggtcat caacgataat gatcgaaatt tcaaacattg tgtctctctg
     2221 tcctgttttt caggtaaccg agagtatagc atagaatatt tacccgacaa catagtttat
     2281 tccaacctat cgtattcgta atctccatct ataaactacc atattctcgc cgtcaaacgc
     2341 ctggctatac ttgcctcctg tctggtaaac tgattccaac ccttgggtta attcagaata
     2401 ccaactcggg aaagtgacca ggtagtccgc attctccttc gtcaaccaat tatctatttg
     2461 agcctcatcg cgtatgaatg ggatcacctc tggtgacacc agccccgcca tatccaatat
     2521 tcttcgccca ccgaaatatc ccagcgcgcc aatatcatgt gcaccgatca acacatctgg
     2581 ctctgtgtac tcctcaatcc agcgcgatgt agctaccatc tcactttcaa taaaagccac
     2641 gtcgcgccca tatgcctggg cgccgagata ccagaacgtt atgcataaca atccaataga
     2701 aaccaaccag gctttgctta gcacgcgcct ccataatatt ggagaattcc aaagaaaaag
     2761 gctcgttgta cctgccaaac cccatagaaa ataggggggc atcatcggca tgacatatct
     2821 gccatgttga taggttacag gtagtctcaa agcataaaca acaagatagc ctattatcca
     2881 gaaagatcca gccacaaccc cccaattgtg atgtcgaaac gaacgataaa aaaaataaat
     2941 aaaaccagga attaacataa aaccgatccc gatattcggt aaggctgctt gttctaataa
     3001 tctcttcccg agcggttcaa ggagttcgac agcgtattca gcttgcttgg catagaatgt
     3061 attaggccag atgacgccag aaagcatccg attccataac aaataagggc tgaaaagcag
     3121 cgaaaatcct actgccaaga gcataattcg gcttacccgt tgataattgc tcgaagcttg
     3181 cataatacat actagcaggg cgggtgctag aagcgtcagc ccatctggac gaatccatat
     3241 gctcaagcca ataaagcccc ccaaaataat ccagttctgg ttatgcgcaa tgagcatgga
     3301 aaatattgcc aatactatca acgaaaataa agcggtctcc attccagata ccgctgccca
     3361 taccaaatgc cactccccca ccaataatgc tcctacaagc aaggccatca tcctgttgct
     3421 cgccggttgc agctttgaga acatccacaa
//
LOCUS       DBNGSX010000584         1755 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1239220, whole genome shotgun sequence.
ACCESSION   DBNGSX010000584 DBNGSX010000000
VERSION     DBNGSX010000584.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1239220"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="AC16CB_16980"
     CDS             complement(<1..238)
                     /locus_tag="AC16CB_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344219.1"
                     /translation="MTNFRGRPRINLPQLGRPSNYNYRVLIAGISLVLLLSLAISTAL
                     SQAPNATWYPVRSFEIDELGISNPAGLAFSPQDNA"
     gene            complement(718..1425)
                     /locus_tag="AC16CB_16985"
     CDS             complement(718..1425)
                     /locus_tag="AC16CB_16985"
                     /inference="COORDINATES: protein motif:HMM:NF015534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HIR3344220.1"
                     /translation="MLALVGSGEYLPDMETVDRFLLDELGTAPRVVCLPTAAGSEGPQ
                     RIAYWSELGVNYFARLGASAEALPVITRQDAQNPILADRVKESNFIYLSGGRPDYLLK
                     TLQDSLVWQAIQDVLIRGGILAGCSAGAMVLGRWIPGFPGMKPAFNLLEEAIVLPHFD
                     EIPNWMVRLILIWVVRRGRMVGVPGFTALVVKGDACKVVGKGNVTIWDSSGKQDYTPG
                     DLLAWSTRTTMGQNQPD"
BASE COUNT          461 a          427 c          414 g          453 t
ORIGIN      
        1 aggcgttgtc ctgggggctg aaagccaggc cggcagggtt ggagatgccg agctcatcga
       61 tttcgaagga gcgcacgggg taccaggttg cgttgggcgc ctgggacagg gctgtggaga
      121 tagccaggga gagcaatagc accaggctga tcccggcgat caatacacgg taattatagt
      181 tacttggacg acccagttgg ggcagattaa ttctaggccg tcccctaaag tttgtcatct
      241 gatgaaatta ttcttttaac caatatgtgc tcacccggtg tacggtgaaa agtaatgatc
      301 tatgatccca gcttttttcg atatggattt ggtataaatg tgatctgcct gcttccattt
      361 gatgtgctat ggctatatcg tgtgcaattc tcacttgtcc caacccctaa caatctagga
      421 gagaatgtac gcgctaataa agtgcggtga ccggtgtttc agtcctgcat atgtaataac
      481 gcctggatac ggcttttatt acacatgggt gtcatcccat aagccgcaaa cgcaaccaac
      541 tatatatagg tcatagctcg tatcactcag taggataacc ctccttaaat gagtgtgttt
      601 gtccaaaata ttatagatta atcttatccg attttagatt aagaaattgt tggaatttaa
      661 atgctgctcg taacaagtgt attaccctgc attcagggag cctacaagtg ccagacatca
      721 atcaggttga ttttgaccca tagttgttct tgtactccaa gccagcaaat ctccgggagt
      781 gtagtcttgc tttccagagc tatcccaaat ggtcacatta cctttcccta ctactttgca
      841 agcgtcccct ttcaccacca aagcggtaaa gccgggcacg ccaaccattc tccctcgccg
      901 gactacccag attaggatca agcgaaccat ccaatttggg atctcatcaa agtgtggcag
      961 gacgatcgcc tcttccaaca agttgaaggc aggcttcata cctggaaaac cggggatcca
     1021 tctccccagc accatcgccc ctgcgctaca tccagccagt atgccgccgc gtataagcac
     1081 atcttggata gcttgccaaa ccaggctatc ctgcagggtt tttaagagat aatccggcct
     1141 gccgccagac aggtagataa aattggattc cttcacgcga tctgcaagga tggggttttg
     1201 ggcatcctgg cgggtaatga ctggcaatgc ctctgcgctt gctccgagcc ttgcaaagta
     1261 gttcactccc aactctgacc aataggcgat acgctgcggt ccttcgctgc cggcagctgt
     1321 gggaaggcag accaccctgg gtgctgtgcc aagctcatcc aataagaatc gatcgacggt
     1381 ttccatatcc ggcaaatatt ctccactgcc taccaatgcc aacattgttc acctcgggtt
     1441 cgtgatcggc tcgacctgtg ccaggaagat gccaagccat gcaataaaaa gaaattttag
     1501 cggatgtttt cccaaatgcc aattccagaa gttacacagt tccagaatga taggggattt
     1561 gggcaggctc ttagttatat aataaaattg aatctaaact gccaatgact tcatcaggaa
     1621 cttgagacct ggaacaacgg tctcaaggga atttgtgatc attttttata atgaaggatg
     1681 gaaaaggatg tcaactcaac cgtcggattt ggatggactt acgatcttag gaggtaaggc
     1741 tcaacccagt aagca
//
LOCUS       DBNGSX010000585         1875 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1862207, whole genome shotgun sequence.
ACCESSION   DBNGSX010000585 DBNGSX010000000
VERSION     DBNGSX010000585.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1862207"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="AC16CB_16990"
     CDS             <1..356
                     /locus_tag="AC16CB_16990"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3344221.1"
                     /translation="DPLNETVLFRIAQEALTNVTRHSGADKATVVLAFEPCQIRLAIQ
                     DEGSGFTPNESSHPPRGWGLVGMRERAESVGGSFHIHATPGIGTLIEVIVPLSDTQPA
                     PIEENNHEPNPVDAC"
     gene            328..1017
                     /locus_tag="AC16CB_16995"
     CDS             328..1017
                     /locus_tag="AC16CB_16995"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3344222.1"
                     /translation="MSPIRLMLVDDHEIVRTGLRTFLQSQEGFNIVAEASSGKQALER
                     AQEAQPDVVIMDITMPQMDGLEATRRLKRILPDCHVLALTVHEDKQYLFEMLAAGASG
                     YITKQSAAEDLVAAIHSVASGNVYLQPALARWLLEDYRRLLSHAPPATGPASEERKLG
                     KGLEILSERELQVLELLSQGLTNPQIGVKLGISPKTVARHRERIMNKLDMHSSTELVK
                     FAIRTGLIDVN"
     gene            1212..1733
                     /locus_tag="AC16CB_17000"
     CDS             1212..1733
                     /locus_tag="AC16CB_17000"
                     /inference="COORDINATES: protein motif:HMM:NF015085.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HIR3344223.1"
                     /translation="MEESITTTTQTSTAGRAKFIVGGLLIIAAIVYLIVSSTQASAQY
                     FLTVEELNAKVDSVAGRELRISGAVIGDSIQYDPQTLTLTFTVAHVPGDNKEIEAQGG
                     LATALHAAVMDPNRPRLEVVYTGVKPDLLRDEAQAIMTGRMGDDGKFHAEELLLKCPT
                     KYEEAVPQQIDQG"
BASE COUNT          447 a          507 c          484 g          437 t
ORIGIN      
        1 tagacccgct gaatgaaact gtgctatttc gcattgccca agaagccctg accaatgtca
       61 cccgccattc aggtgccgac aaagccacgg tggtcctggc atttgaaccc tgccagatca
      121 ggttggcaat ccaggacgag ggttctggct ttacccccaa cgaaagctcc cacccgccac
      181 gtggctgggg tttggtaggt atgcgtgagc gtgctgagtc cgtcggtggc tcgtttcata
      241 tccatgccac ccctggaatt ggcaccctaa ttgaagtgat tgttccactt tcagataccc
      301 agccagcacc gatcgaggag aataaccatg agcccaatcc ggttgatgct tgttgatgac
      361 cacgaaattg tgcgcaccgg cctgcgcaca ttcctccaat ctcaagaagg ctttaacatt
      421 gttgcggagg cttccagcgg caagcaagcc cttgagcgcg cccaggaagc acagcccgat
      481 gtggtcatca tggatatcac gatgccgcaa atggatgggt tagaggcaac tcgacgtctc
      541 aagaggatac tgcccgactg tcatgtgctg gcattaaccg ttcacgagga taaacaatac
      601 ctgtttgaga tgctggcggc gggagcttca ggctacatca ccaagcagtc tgctgccgaa
      661 gatctggtag ctgccataca ctccgttgcc agcggcaacg tgtatttgca acctgcactg
      721 gcccgctggt tgttggagga ctatcggcgg ctactatctc atgcaccacc agcgaccgga
      781 cctgcttctg aagaacggaa gctgggcaaa gggctggaaa tcttgagcga gcgtgagctt
      841 caagtgcttg agcttctctc acagggactc accaatcctc agattggggt caagctgggc
      901 atcagcccaa agacggttgc ccgccatcgc gagcgtatta tgaataagct ggatatgcac
      961 tccagcacgg agctggtcaa attcgccatc cgtaccggcc taattgatgt caattaaagc
     1021 ttgagaactt ctgccgttga agtacttgaa tcatcaaccg tgtagttttt ggttctaatg
     1081 ttttttttgg cacaatctca gctattctta atctgaattt gtaacaatgt gaattagact
     1141 attaaccgca atccaagcgg cgattttatg ccgcccttag atcattttac tgaactgatg
     1201 aggtgttcaa catggaagaa agcataacca cgacaacaca gacctccact gcgggacgcg
     1261 ccaagtttat cgtaggcgga cttttaatta tcgccgccat cgtgtacctg atcgtctcct
     1321 cgacccaggc cagcgcccag tatttcctta ccgttgagga gctgaatgcc aaagtcgata
     1381 gtgtcgccgg gcgcgagttg cgcatctctg gcgcagtgat cggtgattcc atccaatatg
     1441 atccccagac gctgacgctc acctttacgg ttgctcacgt gccgggagat aacaaagaga
     1501 tcgaggcgca gggtggtctg gcaacagcac tgcacgccgc cgtaatggac cccaaccgac
     1561 cgcgcttgga agtggtctac actggtgtga aacctgacct gcttcgtgac gaagcgcagg
     1621 caattatgac tggacgcatg ggcgacgatg gcaagttcca cgccgaagaa ctcctgctca
     1681 agtgcccgac aaaatacgaa gaagctgtgc cccaacagat cgatcaaggt taaactttat
     1741 catggctgct aacatcggat acggtgcgct tgtcattaca tttgtaatat ctttgtacgg
     1801 catctttgca gcgatttacg gcgtgcgcaa gagctctcct gcctgggtag acagtgctcg
     1861 caacgccatg ctgct
//
LOCUS       DBNGSX010000586         2438 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_824052, whole genome shotgun sequence.
ACCESSION   DBNGSX010000586 DBNGSX010000000
VERSION     DBNGSX010000586.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_824052"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="AC16CB_17005"
     CDS             complement(<1..820)
                     /locus_tag="AC16CB_17005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344224.1"
                     /translation="MKRVVFFALVIAFLLVPAFTPVVLSKAAEKTHFPLVVRNRTGGE
                     VTIALVRTDGPGVYWLTVPPGAEGRFEVRSGSYTHTTVACGKTESGVLQVTRGLRLIF
                     TPCPGVAPNAGAPTFEKIHLSDAPSGKLWSYQYGPARGGAASGGDSAVGGACQYTATA
                     EVTIYTRPSTSADEFSTQGAGFSIQPSARTSNGWLGFDPGVAQAANIGPFRLRWLPPG
                     SGTQTAGCNSLPVVWAPLPGICYDMPMGDTKVYASPDTSATVLFVLHLGEFAELL"
     gene            complement(971..1042)
                     /locus_tag="AC16CB_17010"
     tRNA            complement(971..1042)
                     /locus_tag="AC16CB_17010"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1008..1010),aa:Ile,seq:cat)
     gene            complement(1048..1119)
                     /locus_tag="AC16CB_17015"
     tRNA            complement(1048..1119)
                     /locus_tag="AC16CB_17015"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1085..1087),aa:Gly,seq:tcc)
     gene            complement(1187..1381)
                     /locus_tag="AC16CB_17020"
     CDS             complement(1187..1381)
                     /locus_tag="AC16CB_17020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344225.1"
                     /translation="MGQTRGEVARIWLFLAPAVCLVGAYGLLRSFRARRYSIYLLLGA
                     QWVIAVLIFQFLDYGCKLCP"
     gene            complement(1362..>2438)
                     /locus_tag="AC16CB_17025"
     CDS             complement(1362..>2438)
                     /locus_tag="AC16CB_17025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344226.1"
                     /translation="LILTMFVSRQVLRAPHRGRWNVVLILILGYASQLSFGIAQGQGF
                     DSLRLKYVSSGMSVFSKNACSDVGVLESVRRYDELYGTGFWEGTKPPGLQAIFLLIGN
                     GVELIQPGASAQACFEALTTFAAYIFPLIAVLVVFPLVYVGRFLLNENTGAYLAAVFY
                     FTLPNFTQFPLFHDQFLFPLIFMSVMALMLFASQKRSFWLSTLTGVALYLSAFTSFSL
                     LPVLGFALVWFVLAFISSKEAAWMDFLKVLLGLVLGLALAALIFYQFLEYDPIARYQA
                     AFAQHREIKFFNTDQGQFWSILLQNNLEYFILIGFPHGCVVHLPILRFAAVVCGEPPK
                     PHGYIHRHALRDLYSAQPDGTDQG"
BASE COUNT          624 a          687 c          686 g          441 t
ORIGIN      
        1 ccagcaattc ggcgaattcc ccgaggtgca gcacgaagag aaccgtagcg ctcgtatccg
       61 ggctggcata gaccttggta tcgcccatcg gcatgtcgta gcagattccc gggagcggtg
      121 cccagactac cggcaagctg ttacagcccg cggtctgcgt acccgaaccc ggcggcagcc
      181 agcgcaagcg gaacggcccg atgttggccg cctgcgccac cccgggatca aagccgagcc
      241 agccgttcga ggtcctggcg ctgggctgga tcgaaaagcc tgcgccctgc gtcgaaaatt
      301 catcggccga agtgctcgga cgggtataga tggttacttc tgcggtggct gtatactggc
      361 aggcacctcc gacagcgcta tccccacccg atgctgctcc ccctctggcc ggtccgtatt
      421 ggtaagacca cagctttcca ctgggagcat cgctcaggtg gattttctca aaggtcgggg
      481 cacctgcatt gggtgcaacc cccgggcagg gggtaaagat caacctcaat ccccgggtga
      541 cctgcaacac gccggattcg gttttgccac aggcaaccgt ggtgtgggta tagctgccgc
      601 tccgtacctc gaaccgcccc tcagcgccgg ggggtactgt cagccagtat actcctgggc
      661 catctgtgcg caccagggca atggtaacct ccccgccagt cctgttgcgg actaccagcg
      721 ggaaatgggt tttctccgcg gccttggaga gaacaaccgg cgtaaaagcc gggaccagta
      781 aaaaggcgat gaccagggca aagaatacaa cccgtttcat gcttccctca ctttcactgg
      841 tccagggaga accctggatt tgagatcata tcaactaatg attatttgac cgaattatca
      901 ggtaggcgca agcgacgttg atactcttgg acatcgttgg tgcatcccct ctaatgtggc
      961 gttgccagtg tgggcccggc aggattcgaa cctgcgacca aacggttatg agccgtccgc
     1021 tctgccgctg agctacgggc ccgaaggagc gggcgaccgg attcgaaccg gcgaatgtca
     1081 gcttggaagg ctgatgcctt accacttggc gacgcccgcg ttgggatgtt tattttacct
     1141 gcggtcgagg cttctggcaa gagcacaccg aagaagctcg ggtgtgctat gggcagagct
     1201 tgcaaccgta gtccagaaat tgaaaaatta acaccgcaat tacccactgc gccccaagga
     1261 gcaggtagat agagtaacgc ctggctcgaa atgacctgag caacccgtaa gcccccacca
     1321 ggcagacagc cggcgccagg aacagccaga tgcgcgccac ctcacccctg gtctgtccca
     1381 tcaggttgag cgctatatag gtcacgaaga gcgtgacggt gaatatatcc atgcggcttt
     1441 ggcggctccc cgcaaacgac agcagcgaag cgaaggattg gtagatgaac aacgcagcca
     1501 tggggaaagc cgatcaggat gaaatactcc aaattgttct gcaacagtat cgaccagaat
     1561 tgtccctggt cggtattgaa gaattttatc tcgcgatgct gggcaaaggc agcctggtac
     1621 ctagctatgg ggtcatactc caagaactgg tagaaaatca aggcggcgag ggccagtccc
     1681 aagacgaggc ccaacaagac cttcaggaaa tccatccagg cagcttcttt ggaagatata
     1741 aatgccagca caaaccacac cagcgcaaaa cccaacacgg gcaacaggga gaacgaggta
     1801 aaagcagaca gatatagagc cacaccagtg agcgtggaga gccaaaaaga ccttttttgg
     1861 ctggcgaata gcatcagcgc catcacagac ataaaaatca ggggaaacag gaactggtcg
     1921 tgaaacaggg gaaactgggt gaagttcggc aaggtgaaat agaaaaccgc cgccagataa
     1981 gcgcctgtat tctcattcaa taggaaccta ccaacataaa ctaacggaaa aacaaccagc
     2041 acggcaatga gagggaatat gtaggcggcg aaggtggtca aagcttcaaa acaggcctgc
     2101 gctgaagcgc ccggctggat gagctccacc ccattgccaa taagcaggaa gatagcttgc
     2161 agcccgggcg gcttggtccc ctcccagaag ccggtgccgt acagttcgtc gtagcgcctc
     2221 acgctttcca agactccgac atcgctgcaa gcattttttg aaaagacact cattccagac
     2281 gagacgtact tcaagcgcag agagtcaaat ccctgaccct gggcgatgcc gaagctaagc
     2341 tgggaggcat aacccaaaat aagaataagc accacattcc agcggcccct gtgtggggct
     2401 cgaagcacct ggcgggagac aaacatcgtc aggattaa
//
LOCUS       DBNGSX010000587         3466 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1654449, whole genome shotgun sequence.
ACCESSION   DBNGSX010000587 DBNGSX010000000
VERSION     DBNGSX010000587.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1654449"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="AC16CB_17030"
     CDS             <1..695
                     /locus_tag="AC16CB_17030"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR3344227.1"
                     /translation="PLAIVFENQDLIVVNKPAGMVVHPGAGHTRGTLVHAALAHAPDM
                     AGIGGEGRPGVVHRLDKDTSGLILLAKNDAAHRRLQEQFRQRQVVKGYMALVDGHPPT
                     PSGRIEAPIGRDPAQRRQMAVVAAHKGRAAVSEYHTLESFPKHALLEVHPITGRTHQI
                     RLHLSFLGCPVVGDSIYGRRKPSLPISRHFLHAARLTIRLPKENAPRTFEAQLPTELV
                     QILDDLRSSSEL"
     gene            692..1729
                     /gene="ltaE"
                     /locus_tag="AC16CB_17035"
     CDS             692..1729
                     /gene="ltaE"
                     /locus_tag="AC16CB_17035"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HIR3344228.1"
                     /translation="MNYIDLRSDTVTKPTPAMWEAMARADVGDDVYGEDPTINRLQEM
                     AAERVGKEAALFLPSGTKGNLSAILAHCGRGDEVILGDKAHSFLLEAGGISALGGIHS
                     RQLPNQPDGTLALDDIRLAIRSDDAHFPISRLVTLENTHNRCGGAALSVEYTRSVGDL
                     AHEHDLLLHIDGARLFNAAVAQGVDACALAAPADSVTFCLSKGLCAPVGSLLCGSTEF
                     INRAHRMRKQLGGGMRQAGVLAAAGIVALETMVERLAEDHQRARMLAEGLATIHGLVL
                     DPDTPRTNMIFCSLADTVLMNASQVVAALAERGVRVGAVGTRRFRLVTHYWIGDGDVS
                     QAVEAFREVLV"
     gene            1911..2747
                     /locus_tag="AC16CB_17040"
     CDS             1911..2747
                     /locus_tag="AC16CB_17040"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR3344229.1"
                     /translation="MSEFITLEQIDQATNHIRSRITTSPRVGLILGSGMGILAESVEN
                     GTQISANELPNWPVSTVEGHQGRLVIGKLEGKQVMVMQGRSHYYEGYSMGQITLPVRV
                     MQRLGLEILVVTNAAGAVNPSFEPGDLMLITDHLNLVGMTGLNPLRGPNLDEFGPRFP
                     DMSQAYDRQLGKIARQVAVENQIGLQEGVYACLSGPSFETPADLRFLRAAGADAVGMS
                     TVPEVTVARHGGLRVLGISGISNKANLDGSTITTHEEVLESGRVITPKLLAILRGVLR
                     NV"
     gene            2814..>3466
                     /locus_tag="AC16CB_17045"
     CDS             2814..>3466
                     /locus_tag="AC16CB_17045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344230.1"
                     /translation="MVEALRFFRAYEIWIYLILGLGGLIYIRKFILAWQELREAAFGL
                     ERESALGRLNQSASILVVVLALTITEFFLVSFVAPSLPEAIPLLTPTLDLLATTTSTL
                     PPTTLEAGTSPIQATATVASTPAGLAEGCIPGQVMITSPPSGTEISGVVVISGTASIT
                     NFGFFKLEMKRVEESNWLTILAGNEVKVDDTLGAWNTSLLTPGDHELSLVVTDNQGNS
                     "
BASE COUNT          752 a          990 c          995 g          729 t
ORIGIN      
        1 tcccgctagc gatcgttttc gaaaaccaag acctgatcgt agtgaataag cctgctggca
       61 tggtggtgca ccctggggcg ggtcataccc gaggcaccct ggtacacgcc gccctggcac
      121 acgcccctga catggcgggc attggcggcg aggggcgtcc gggcgtggtg catcgcctgg
      181 ataaagacac ttctggcctg attttgctcg ctaagaacga cgccgcccac cgccggctac
      241 aagagcaatt ccgccagcgc caggtagtca agggctacat ggcgctggtt gacggacacc
      301 cacccacccc cagcggcagg atcgaggcgc ctatcgggcg agacccagcc cagcgtaggc
      361 agatggcagt tgtcgctgcg cataagggac gagccgccgt cagcgaatac cacacgctgg
      421 aaagctttcc caaacacgcc ctgctggagg tgcacccgat caccgggcgc acacaccaga
      481 tccgcctgca tttgagcttc ctgggctgcc cggtggtggg tgactcgatc tacgggcggc
      541 gcaaacccag cctgcccatc tcgcgccact tcctgcacgc agcccgctta accatccgcc
      601 tgcccaaaga aaacgccccg cgcacatttg aagcccaact gccaacagag ctcgtgcaga
      661 tattagacga tttaagatca tcgagtgaac tatgaactat atcgacctgc gctcagatac
      721 cgttacaaaa ccaaccccag ccatgtggga agccatggcg cgcgccgacg taggcgacga
      781 tgtgtacggt gaagacccta ccatcaaccg cctgcaagaa atggcagccg aacgagtggg
      841 taaagaagca gcccttttct taccgtctgg gacgaagggc aacctgtctg ctatcctggc
      901 tcactgcgga cggggggatg aggtcatcct gggggataaa gcccacagtt tcctgctcga
      961 ggccggcggc atctctgccc tggggggcat ccattcccgc cagcttccaa accagcctga
     1021 tggcaccctg gcactggacg acatccgcct cgccatccgc tccgacgatg ctcacttccc
     1081 tatatcgcgc ctggtaacgc tggagaacac ccacaatcgt tgtggtggcg cagcgctcag
     1141 tgtcgagtac actcgaagtg taggcgatct ggcacatgaa cacgacctgc tcctgcacat
     1201 tgatggagcg cgcctcttca acgcggctgt tgcccagggg gtagatgcct gcgccctggc
     1261 tgcgccagcc gattcggtga ccttttgcct gagcaaaggg ttgtgcgcac cggtgggctc
     1321 gttgttgtgc ggctccacgg agttcatcaa ccgagcgcac cgcatgcgca agcagttggg
     1381 cggtgggatg cgccaggcag gtgtcctggc ggcagctggc attgtcgctt tagagaccat
     1441 ggttgagcgc ctggctgaag accaccaacg cgccagaatg ttagccgaag ggctagccac
     1501 catccatggt ctggtattgg accccgatac acccagaacg aatatgatct tttgctcatt
     1561 agccgataca gtgcttatga acgcttccca ggtcgtcgca gccctggcag agcgcggtgt
     1621 gcgcgtgggc gcagttggaa cgcggcgctt tcgcttggtg acacattact ggatcgggga
     1681 tggggacgta tcccaagccg tagaggcgtt tagggaggta ttggtttaat agagagcggt
     1741 cgctcattta ccctagtata ggagattttt ggacttgctc ctatcgcccc gtgaaggagt
     1801 gggggggaca ttagagcgat tgccctgcgg ggtaggaaag cacgaagaga tacgcatgtt
     1861 ataattgtca actgcggtgg ggtcatatcc caattcaatg gaaacgatct atgagcgaat
     1921 tcatcacact agaacaaatc gatcaggcca ccaaccacat ccgctcccgt atcaccacat
     1981 ctccccgcgt tgggctaatc cttggctcgg gcatgggcat cctggcagaa tctgttgaaa
     2041 atggaacaca aatctctgcc aatgagttgc caaactggcc agtatccact gtagaaggtc
     2101 accagggacg cttggtcatc ggaaaactag aaggcaagca agtcatggtc atgcaaggcc
     2161 ggtcgcacta ctatgagggc tatagtatgg ggcagatcac gctgccagtg cgcgtcatgc
     2221 agcgcctagg gctggagata ctggttgtca ccaacgcagc tggagcggtc aaccccagct
     2281 tcgaaccggg cgacttaatg ctgatcactg atcatctaaa cctggttggc atgaccggtc
     2341 tcaatccgct gcgcggccct aacctggacg agtttggacc acgcttcccg gatatgagcc
     2401 aggcatatga ccgtcagctc ggcaagattg cccggcaggt ggcggttgaa aatcagatcg
     2461 gattgcaaga aggcgtttac gcctgcttgt ctggtccatc gttcgagaca cccgccgatt
     2521 tgcgttttct acgtgcagcc ggggctgacg cagtgggcat gtctactgtg cctgaagtga
     2581 ctgttgcccg gcatggtggg ttgcgtgtgc tgggtatctc gggcattagc aataaggcta
     2641 atctggatgg cagtacaatc accacacacg aagaggtatt ggaatctgga cgggtgatca
     2701 ctccgaaatt attggccatt ctcagggggg tgctgcgcaa cgtataacct tatcccttcc
     2761 tttggagtcc ggcgtccgct agcttcaaga ttgcccaggt atcgaatttc tttatggtag
     2821 aagcgctccg tttttttcga gcctacgaaa tatggatcta cctgattcta ggcttggggg
     2881 ggctaatcta tatccgtaaa tttatactgg cctggcaaga gctgcgcgag gcggcttttg
     2941 gcttggaacg cgagagtgct ctggggcgcc taaaccaatc ggccagcata ttggtggtgg
     3001 tgctggcgct tacgattaca gagttttttt tggtatcttt tgttgctccc tctcttcctg
     3061 aggctatccc cttgctaacc cctacactgg atttgctggc tacaaccact tccacactcc
     3121 ctccaaccac actcgaagca gggacgtcac ctatccaggc aaccgccacg gtagcctcca
     3181 ccccagcggg attggccgag ggatgcatcc ccggacaggt gatgattacg tctcccccaa
     3241 gcggcaccga aattagcggg gtggtggtta tcagcggcac cgccagcatt accaacttcg
     3301 gtttctttaa gctggagatg aaacgggtgg aagaaagcaa ctggttgacc atcctggccg
     3361 ggaatgaggt caaagtagat gatacgctcg gagcttggaa caccagcttg ctcaccccgg
     3421 gtgatcacga actcagcctg gtggtcactg acaaccaagg gaactc
//
LOCUS       DBNGSX010000588        10069 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1239728, whole genome shotgun sequence.
ACCESSION   DBNGSX010000588 DBNGSX010000000
VERSION     DBNGSX010000588.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10069)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10069)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10069
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1239728"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="AC16CB_17050"
     CDS             <1..356
                     /locus_tag="AC16CB_17050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344231.1"
                     /translation="MDGAKTFRDEGGNSSYGQHEPGRISNKSIGRFRQVLSKRQIAFM
                     QSMAGREMEAFGYILDKIHLSPVEQSLYYLLDVPINLARMAAWLLREAYLDQIGRPVP
                     SYRIVAGPDAIKAGA"
     gene            445..1473
                     /locus_tag="AC16CB_17055"
     CDS             445..1473
                     /locus_tag="AC16CB_17055"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR3344232.1"
                     /translation="MPKNYLVTGGAGFIGSNYAARLLQRGENVTIYDNLSRAGGPLNL
                     RWLEDTFGKGSFRLVEGDLRDAALLTENTRDADVIVHLAAQVAVTTSVVDPRDDFEIN
                     ALGTFNMLEAARLSERKPAILYASTNKVYGGMEDVRVDENESRYHYADYPYGVPETFP
                     LDFHSPYGCSKGAGDQYVRDYHRIYGLPTVVFRQSCIFGPRQFGVEDQGWVAWFIIAT
                     LTGKPITIYGDGKQVRDVLYIDDLLDVYDAALIQIDDVAGEVFNIGGGPNNVMSVWGE
                     FGPILKRLLGSKIQVSWGDWRPGDQKVYISDIRYAEKKLGWKPKINVEAGVECLFEWA
                     RENRKLFT"
     gene            1621..3030
                     /locus_tag="AC16CB_17060"
     CDS             1621..3030
                     /locus_tag="AC16CB_17060"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR3344233.1"
                     /translation="MASSIGIVAAYLIVKGEWQIAFSLALSIPGLIILHKYPFLGVII
                     WLILTPFLLHTDTGAERRVYWIIHRGLPPLTVILIFLKSALYIERRKMPKLGMPELLM
                     AGYVVASLLSIYFQSSDPLATAYRYYDRVISPMCLYLIIRLLAPNQKEMQWLIPVAFF
                     LSMTQSIIGIVAWFAPHYLPNDWLDKAGSRTVGTLINTSVFTTAITFSGLLSLQGAFF
                     SNSRIFRNSLIVAFLLSIYSIFIAFSRAGWLAGILVILGLLYLYPKFMLKLGLLFIPL
                     AFILAGVFADQIYWARERLLSERAENSALSRLPVFYAAYRMFQIKPIFGWGYDNFDEF
                     DRRYQGRVADLANDNKDHASHNTYLTIIAEQGIVGLITFLGPAIWWLIYTLKILPGLP
                     KYTFWSSKLLILLWLVILTQFINNNFMNLLVVFGHGIWWVVLGLIANIVHAYRLPADA
                     GKIETRIPSAWIFRMSNET"
     gene            3048..3983
                     /locus_tag="AC16CB_17065"
     CDS             3048..3983
                     /locus_tag="AC16CB_17065"
                     /inference="COORDINATES: protein motif:HMM:NF024860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HIR3344234.1"
                     /translation="MQKSLERIWVSFLDSPRFRRFANQRVGMAIFRSPAYRSILMQYN
                     YVSSYYKCLAHPSAYKNVKTFCLFIGHNKSGTSLIGSLLDAHPNVILADEVNALAYVS
                     AGFKRDQIYHILLKGSRRELMKGRVTARRLKPYSYLVPGQWQGRYSTLQVIGDSTSGS
                     STQRFAREPDLLQRLERSMAGVEVKFIQVIRNPFDPISAMMIRGKRSFENAIEHYFAN
                     SESLVELRKRIDSSNLMPVRYEDFINGSGSDLENICHYLGINADSDYLKACQSILHKH
                     PDKSRQMVNWEDKWIRIVEDKIGQYDFLQGYSFNE"
     gene            4172..5560
                     /locus_tag="AC16CB_17070"
     CDS             4172..5560
                     /locus_tag="AC16CB_17070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HIR3344235.1"
                     /translation="MQGLTDLDLLVDPEDAQVFKQVLAEHDIKPFLAAPGKRYPALED
                     YMGFDPSSGKFFHLHVHYQLVLGEQFVKNYRLPLEDQFLDSIQYRSGVKIPAPELELI
                     VLSIRALLKYRDRDAIKDILSIRSPGIPAHIMGEINWLLNQTCLNKVSKTLDEVADAI
                     SPDLILDFLNTVMMTPRAGYRLFRLRQSVRRAISVYQRHNRLWASLTYFKEFWRRRKS
                     FLRFTPERKMTNPRGGLTLALVGVDGAGKTTISRELAAWLDWKLDIHTYYLGSKQPSW
                     MSDRSYLLFRIARRSHRSISKVLGEKNFISRLIEALRQALLYSHYLFTGYDRYRRYLA
                     GNRNAKNGSISLYDRYPLEAPLDGPQIHLASENDPWRIGDIFSKLERKLYQEIKMPDL
                     LFVLEVSPEVSLERKPDHDLAAIEAKNIALGKLATFIEASPKKVRVSYINASLPLEDV
                     LGKIKQEIWGAL"
     gene            5589..6386
                     /locus_tag="AC16CB_17075"
     CDS             5589..6386
                     /locus_tag="AC16CB_17075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344236.1"
                     /translation="MIIEFIGTPGAGKTTLLPVVMDYFDGLGFRAYTVVQAARPFAAR
                     TLLGRAISRLAPAGLRQQLLWQVFYHLSKLYRLRFMLKNRELIYSVQRFQRKRPITSQ
                     DREHVLYWFLHLIGYYEFLKSQLEPNEALIFDEGFIHRVVQLFASEVEEPDPDQITAY
                     INLIPKPDLVVFPYSPKEICEKRVYARGIWDRFRLKSREEISRYMANSHEIVNLAVDY
                     IQKKGWVVLSVDNGGEDPGKAKAELHKELSSSFTYSFQIPEFQPQAQ"
     gene            6394..7512
                     /locus_tag="AC16CB_17080"
     CDS             6394..7512
                     /locus_tag="AC16CB_17080"
                     /EC_number="2.7.-.-"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase enzyme family protein"
                     /protein_id="HIR3344237.1"
                     /translation="MVSVSRIYYLPRPSRFTAYIQSRFRSPDIELNTVNEILAHYGVT
                     LTATPRNLPNTRRNRNLIINTSAGKKILKLYRKDWRTSTIVFEHSVLTRLAQLSFPAP
                     RLVSTTDGATYVNKSKNNYALFDFADGTNYSTSFLLRAHRLRLMAIAGQTMASLHRQL
                     KGFLPEGQHHLGFKSYVDGRHRDMNWYREKVDELESKSLTLTDPQEIAIAKELIQQRI
                     YVLDELSKLDQELSSAPLLRTIIHGDYGLHNILIQDSAKATPVDFELTRLEWRLSDLV
                     SCLSRIRFATGEYDFESLQWFMDGYQTEFPFSDEEWDLFPQVWRHYKLKGAIGYWVSY
                     FETGGPTRKLNSARDAIAQAKWALKHPDQLLSIRTKKT"
     gene            7596..8822
                     /locus_tag="AC16CB_17085"
     CDS             7596..8822
                     /locus_tag="AC16CB_17085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3344238.1"
                     /translation="MLRSKINVMQVVSNLDVGGGQEVVRTLAENLAESGCAPVVCTFK
                     DGPLREPIESLGIPVEVLPERRANILALPSFLMEMLRIRRDLANLIQKYEIDVIQTHL
                     LRSLDFLVLSLRFGRDLLVFWTFQNAVFDLREDHLTRHKWLLKPKRFAHRLLYRLGSR
                     WVTGFIAVSQDTKKAILEYLGHLPDDKITVIFNSVDVKRYHREVDRGSIRRQLGLADS
                     DHVMSVVATFKKQKGHCYLIEATSLIIQEMPDIHILFIGDGELREELMTQTKSLGLER
                     NIHFLGTRKDVPELLAASDYFILPSLWEGLSMALVEAMASQIPVIATDVSGTKQVIIP
                     GETGILVPPGNAQQLANAMLQIVSDPEKAKAMAIAAKQRVEKYFSAQKQAKDHIALFE
                     RELNKTNWQPALSIRR"
     gene            8826..9857
                     /locus_tag="AC16CB_17090"
     CDS             8826..9857
                     /locus_tag="AC16CB_17090"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR3344239.1"
                     /translation="MSYDPIFIAGPDRSGTTLIYALLASHPNISMVRRTNMWRYFHKR
                     YGELSQPENFERCLNDMVRFNRMRQLQPDPERIRQEFWQGEPTYGRLFALFHEHVAER
                     RGKKRWGDKSLHTERHADRVLAEYPNAKIIHISRDPRDRYASVRKRHGKDMPRLGAAT
                     GRWLFSMSAARRNLKRYPNNYMIIKFEELASQPEEVLRKVCAFIGEEYTPAMLTMKGA
                     ADHRDKGGNSSFGEIKPGVISKSPIGRFRTVLSKPEIAFIQLFTAREMKALGYSRERV
                     RFSPVEFLKYYLWILPNHMARMFGSMMLTARGIRRGERIPAHRLLDLPVTTPGGYDAK
                     VKETAQNQI"
     gene            9817..>10069
                     /locus_tag="AC16CB_17095"
     CDS             9817..>10069
                     /locus_tag="AC16CB_17095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344240.1"
                     /translation="MMQKLKRPPKIKSSPLAQPRVLILSPYYRVLIAGISLVLLLSLA
                     ISTALSQAPNATWYPVRSFEIDELGISNPAGLAFSPQDNA"
BASE COUNT         2693 a         2388 c         2378 g         2610 t
ORIGIN      
        1 gcatggatgg tgcaaaaact tttcgagatg agggcggcaa tagctcctat ggacaacacg
       61 agccaggccg catttcaaac aaatcaatcg ggcgttttcg gcaggtatta tctaaacgcc
      121 aaatcgcctt catgcagagc atggccggac gcgagatgga agcgtttggc tatatattgg
      181 ataaaatcca tctttcacct gttgaacaaa gcttgtatta cttattagat gtcccaatta
      241 atctggcgcg catggcagcc tggctgttaa gagaagcgta tttagatcag attggccgtc
      301 cggtgccatc ttatcgaata gtcgctggac cagatgccat caaggctgga gcgtaataat
      361 caatctacta ttcattgata cactcacaaa acgctggatt tctccagcgg atagacataa
      421 tatcatttat taacaggaat taatatgccg aaaaactact tagttaccgg aggagctggt
      481 ttcattggct caaactatgc tgcccgcctg ctacaacgag gcgagaacgt aaccatctat
      541 gataatcttt ctcgggctgg tggaccgctc aatctgcgtt ggctggaaga tacctttgga
      601 aaaggttctt tccggttggt ggaaggagac ttgcgcgatg cggccttgct caccgagaat
      661 actcgcgacg ctgatgtgat tgtccacctg gctgcccaag tagctgttac cacatccgtg
      721 gtcgacccgc gcgatgactt cgagatcaat gccctgggca cattcaatat gctagaagca
      781 gcccgcttgt cagagcgaaa accagcgatc ttgtatgcct ctaccaataa ggtatatggc
      841 ggcatggaag atgtgcgggt tgatgaaaac gagtcgcgct atcactatgc cgactaccca
      901 tacggcgttc cagagacctt tccgctggac tttcattcac cttatggctg ttcaaaggga
      961 gcgggagatc agtacgtgcg cgattatcac cggatttacg gcttgcccac cgtcgtcttt
     1021 cgccaaagtt gcattttcgg ccctcgccag tttggagtcg aagaccaggg ctgggtagcc
     1081 tggtttatta ttgccacgtt aacaggtaaa cccatcacaa tttatggcga tggcaaacaa
     1141 gtacgtgatg tgctgtatat cgatgatctt ctcgacgtct atgacgcggc tctgatacag
     1201 atagatgatg tagctggtga agtcttcaac attggtggag gtcctaataa cgtcatgtcg
     1261 gtatggggcg aatttggacc tatactgaaa aggttgctgg gaagcaagat ccaagtatcc
     1321 tggggtgatt ggagacccgg cgatcagaaa gtttacatat cagacatccg ctacgctgag
     1381 aaaaaactgg gatggaaacc caagataaat gttgaagctg gcgtggagtg tttattcgag
     1441 tgggccaggg agaaccggaa actatttacg taagtggact aaggactcct ggtagcgaaa
     1501 tcaaccacat ttgctttgta atcacctgaa atgtcaacca tctcaagtat ttcacctcaa
     1561 gagaataagc tcaaaaacac agtcaagtca ggtctggttt tgaattggct catcatcctt
     1621 atggcatcaa gcatagggat tgtcgcggct tatctcatcg tgaaagggga atggcaaatt
     1681 gcttttagcc tcgcgctctc cattccaggg ctgatcatct tacacaaata cccctttctc
     1741 ggcgtaatca tatggctgat attgacacca ttcctcttac ataccgacac aggcgctgag
     1801 cgcagagtgt actggatcat ccacagaggc ttacccccac tgacagtgat tcttatcttc
     1861 ctcaagtctg cgctttacat cgagcgacgc aaaatgccca aattgggtat gccagaatta
     1921 ttgatggctg gatacgtggt agcaagcctg ttatcaatct acttccagag ttcagatcct
     1981 ctagctacag catatcgcta ttatgatcgg gtcatcagcc ccatgtgcct gtatttaatc
     2041 atccggctat tggcccccaa tcagaaagag atgcagtggt taattccggt cgctttcttc
     2101 ttgtcaatga cccagtcaat catcggtatt gtagcctggt tcgcgcctca ctatctccca
     2161 aatgattggc tggataaagc aggtagtcgc acagttggca ctttaatcaa taccagcgtt
     2221 tttactaccg cgatcacatt ttctggcttg ttatctcttc aaggcgcctt tttttcaaat
     2281 tccaggatct ttcgcaattc attaatcgtt gcgtttcttt tatccatata tagcatattt
     2341 atagcatttt cgcgagcggg ctggctggca gggatcctgg tcattttggg cttactctat
     2401 ttgtatccaa aattcatgct caaattgggc ttattgttta ttcctttagc gtttattcta
     2461 gcaggtgtgt ttgcagatca aatttattgg gctagagaac gactactatc cgagcgagca
     2521 gagaactccg cattgagtcg gctaccagtt ttttatgctg cctaccgcat gttccaaatc
     2581 aagcccatct tcggctgggg ttatgacaat tttgacgagt tcgatcgacg ttatcaaggt
     2641 cgggtggcgg atctagccaa tgacaacaag gatcacgctt cgcacaacac atacttgacc
     2701 ataattgctg agcaaggaat cgtgggcctg atcactttcc tagggccggc tatctggtgg
     2761 ctgatctata cgctaaaaat ccttccggga ttacctaaat acaccttctg gagcagcaag
     2821 ctcctgatcc tactttggtt ggtcatcctg acccagttca tcaacaataa tttcatgaac
     2881 ctgctggttg tttttggtca cggcatatgg tgggtcgtgc tgggcttgat tgccaatatt
     2941 gtgcatgcct atcgtttgcc agcggatgct gggaaaatag aaacccggat tccaagtgcc
     3001 tggatcttca gaatgtcaaa tgaaacttaa cctagcgaga tcatcgtatg caaaaatctc
     3061 ttgagaggat atgggtatct ttcttggatt cgcctcgatt ccggcgattt gccaaccaga
     3121 gagttggcat ggcgatcttt cgtagcccgg catatcgatc gatcttgatg caatacaact
     3181 atgtatcctc ctattacaaa tgcttagcac atccctccgc ctataaaaat gtcaagacgt
     3241 tttgtctctt cattggtcac aataaaagtg gcactagctt gatcggatcg cttttagacg
     3301 ctcacccaaa tgtcattctc gcagatgagg taaacgccct tgcatatgtt tctgctggat
     3361 tcaagcgaga ccagatatac cacattctgc tcaaaggatc gcgccgcgaa ttgatgaaag
     3421 gcagggtgac agccagacga ttgaagccgt attcgtactt ggtacctggt caatggcaag
     3481 gtcgttatag cacattacaa gttatcggtg atagcacatc tggatcttct acccaaaggt
     3541 tcgctagaga acccgacctg ctccaaagat tggagagatc aatggcgggg gtagaagtca
     3601 agtttattca agtaatccgc aatccatttg atcccatcag cgccatgatg atcaggggga
     3661 aacgatcctt cgaaaatgcg attgaacact atttcgccaa ttccgagtca ctggtcgagc
     3721 tacgaaaacg gatagatagc tcgaatttga tgcctgtgag gtacgaggat tttatcaacg
     3781 gctcaggtag cgacctggaa aatatttgcc attatttggg tattaatgca gacagtgatt
     3841 atttgaaagc ctgtcagagc attcttcaca aacaccctga caaaagtcgc cagatggtga
     3901 attgggaaga taaatggata agaatcgttg aagacaaaat agggcagtac gacttcttac
     3961 aaggatattc tttcaacgag taagattact tgcgattatt gattcacggc tcagttcgag
     4021 gactatgata caggatacca ctgttagaat tcagacacct ctcgaagaca agacaaatat
     4081 tcttcaaaca gttgagggtt tattccaggc attaaaccat tctggtatca ggtattgcca
     4141 ctggaaaagc aatttgagat tggaatgggg catgcaaggt ctgacagacc ttgatctgct
     4201 tgtggaccct gaagatgctc aggtctttaa gcaggtgctc gccgaacacg atattaagcc
     4261 gtttttagcc gcccctggta aacgctaccc agcccttgaa gattatatgg gatttgatcc
     4321 aagcagcggc aagttctttc atttacacgt tcattatcag cttgtcttag gcgaacaatt
     4381 tgtcaaaaat taccgcttac cccttgaaga tcaattccta gactcaattc aatatagatc
     4441 gggtgtaaaa atccctgcgc ctgagttaga actgatcgtc ctctctattc gggctctcct
     4501 taagtaccga gaccgggatg ccattaaaga cattctttct atcagatcac ccggcatacc
     4561 cgctcatatt atgggagaaa tcaactggct attgaatcaa acctgcctca acaaggtctc
     4621 caaaacatta gacgaagtag ctgatgccat ttcccctgat ctgatcctgg attttctaaa
     4681 caccgtgatg atgactccca gggcagggta tagactgttt cgattgcgcc agagcgtgcg
     4741 cagagccatc agcgtttatc aacggcacaa ccgcttgtgg gcctcgttga cctatttcaa
     4801 agaattctgg cggcgacgaa aatcgttcct gcgatttacg cccgaaagaa aaatgaccaa
     4861 tcccaggggt ggattgaccc tggcgttggt tggtgtggat ggcgctggaa agacaactat
     4921 aagccgcgaa ttggctgcct ggttggattg gaagctggat attcacacct attaccttgg
     4981 aagcaaacag ccttcttgga tgagcgatcg ctcttacctg ctattcagga tagctcgcag
     5041 gagccatcga agtatcagca aagtgcttgg ggaaaaaaac tttatcagca ggctgattga
     5101 agctctccgc caggctctgc tatacagtca ttatcttttc accggctatg atcgttatcg
     5161 tcgatacctg gctggcaatc gtaatgcaaa aaatggctca atcagccttt atgatcgcta
     5221 tccattggaa gcgccattgg acggaccgca aatccacctc gccagcgaga atgatccttg
     5281 gcggattggc gacattttct ccaagttaga gcggaagctt tatcaagaaa ttaaaatgcc
     5341 cgatcttctc tttgttctcg aagtgagccc agaagtctcc ttagagcgaa aacccgatca
     5401 cgacttggcg gcaatcgaag caaaaaatat cgcactaggg aaactggcca ctttcattga
     5461 agcatcaccg aaaaaagttc gggtatcgta catcaatgcc agccttccgc tcgaggatgt
     5521 cctggggaaa ataaagcagg agatatgggg ggcgttgtaa agcacctttt tttcacttca
     5581 aaccattcat gatcatagaa tttataggca cgcctggggc gggcaaaaca accctcctgc
     5641 ccgtggtgat ggattatttc gatggccttg gctttagggc atatacggtt gttcaagcag
     5701 cccgaccttt cgccgccagg acattgttag gcagggctat cagccggctg gctcctgcgg
     5761 gtttgcggca gcaattattg tggcaagtgt tctaccacct cagcaagcta taccggctca
     5821 gatttatgct caaaaaccgg gaactaatct acagcgtgca gagattccaa agaaagcgac
     5881 cgattaccag tcaagatcga gaacacgtcc tgtattggtt tctccatttg atagggtatt
     5941 acgagttctt gaagtcgcaa ttagaaccga acgaagcact tattttcgat gaagggttta
     6001 ttcaccgggt agtacaactc tttgcctcgg aggtcgaaga acctgacccc gatcaaatta
     6061 cagcgtatat caaccttatc cccaagcccg acctggtagt ctttccgtat tcccctaaag
     6121 aaatatgtga aaaacgcgtt tacgcccgag gcatttggga tcgatttcgt ctgaaatccc
     6181 gtgaagagat ttcccgttac atggccaatt cacacgagat cgtcaatctt gctgtggact
     6241 atatccaaaa aaagggctgg gtagtgcttt ccgtagacaa tgggggtgag gatcccggaa
     6301 aagccaaagc ggagttgcac aaagaactat ccagctcatt tacctactct ttccaaatac
     6361 ccgaatttca acctcaagca cagtaatcta cctatggtca gtgtatctcg tatctactat
     6421 ctgccccgcc catcccgttt cacggcgtat attcaatctc gctttcgttc gcctgacatt
     6481 gagctcaaca cagtcaatga gatactcgcc cattatggtg tgactttgac ggcgactcct
     6541 cgcaatctcc ccaatacccg cagaaacaga aacctcatta tcaatacctc agcaggcaag
     6601 aaaattctaa aactgtaccg gaaggactgg cgcacatcga caatcgtctt tgagcattcg
     6661 gtgcttacaa ggctagctca attgagcttt ccagctccac gtcttgtttc cacaaccgac
     6721 ggcgctacct atgtcaacaa gtcgaagaat aactatgcat tgttcgattt tgctgatgga
     6781 acgaattact ctacaagttt tctgttgcgt gcccatcgcc tgaggctcat ggcaatcgca
     6841 ggccaaacaa tggccagcct ccacaggcag cttaaaggtt ttctgccaga agggcaacat
     6901 cacttgggat tcaaatcgta tgtggatggc aggcaccggg atatgaactg gtatagagag
     6961 aaagtcgatg agctggaatc aaaatcgctt acccttacag atccccagga aatagccatc
     7021 gccaaagaac ttattcaaca acgcatctac gttttagacg agttgagcaa actggatcag
     7081 gaattaagca gcgcgccttt gctgcgaacc attattcacg gcgactatgg tctccacaat
     7141 atccttatcc aggactcggc gaaagctaca cccgtcgact ttgagctgac acgactcgaa
     7201 tggagactta gcgatctggt ttcttgtctc tcgaggatac gatttgctac gggggagtat
     7261 gattttgaaa gcttgcaatg gtttatggac ggataccaga ccgaattccc gttcagtgat
     7321 gaggagtggg atctatttcc tcaggtatgg cggcattaca aattgaaagg tgccattgga
     7381 tactgggttt catattttga gaccgggggg ccaacgcgca aactgaactc tgcccgagat
     7441 gccatcgctc aggcgaaatg ggcattgaaa cacccagatc aactcttgtc gataagaact
     7501 aaaaagactt gatttaaatt tcggctaaag ctacagaata aacttcatag tgctttagtg
     7561 gcctaagatg gtatgaaaag cttaaggacg gatagatgct tcgatctaag ataaatgtga
     7621 tgcaggtggt aagcaacctg gatgtgggtg gtgggcagga agtggtgcgc accttagccg
     7681 aaaatctagc agagtctggt tgtgccccag tcgtgtgtac tttcaaggat ggtccattgc
     7741 gcgaaccaat agaaagcctg ggtattcccg tagaagtgct acccgaacgg cgcgcaaata
     7801 ttttggctct tccttcattt ctcatggaaa tgctgcgcat acggagagac ttagcaaatt
     7861 taattcaaaa atacgaaatt gatgtcatcc aaacgcacct attaaggagc ctggattttc
     7921 ttgttttgag cctgcgattt ggaagagatc tcctggtttt ctggactttc cagaacgccg
     7981 tatttgacct gcgagaagat cacttaacgc ggcacaaatg gttgcttaaa ccgaagcgtt
     8041 tcgcccaccg cctgttgtac cgcttgggat cccgctgggt gaccggtttc atcgctgttt
     8101 cgcaggatac caaaaaagcg attctagaat atctcggtca tctccctgat gataagatca
     8161 ccgtcatttt taacagcgtg gatgtcaaac gctaccatag agaagtggat agaggctcca
     8221 ttcgccgcca attaggatta gctgattctg accacgtgat gtcagtggtt gccacattca
     8281 agaagcaaaa ggggcactgc tatttgattg aagccacatc cctgatcatc caggaaatgc
     8341 ccgatataca cattctgttc attggcgatg gcgaattacg agaagagttg atgacacaaa
     8401 cgaaatcctt agggcttgag cgaaatatcc atttcttagg tacccgcaag gatgtgccgg
     8461 aattattagc agccagcgat tacttcatcc tgccctccct ttgggagggg ctttcgatgg
     8521 ctctggtgga agccatggcc agtcagatac cagtgatcgc gacggatgtg tcagggacca
     8581 agcaggtgat cattcccggc gaaaccggta tcttagtccc acctggtaat gcacagcaac
     8641 tagccaacgc gatgctacag atcgtgtccg acccagaaaa agccaaggca atggcaatag
     8701 cagccaagca acgggtagaa aaatatttta gtgctcaaaa acaggctaag gaccatatcg
     8761 ctctgttcga aagagagctg aataagacga actggcaacc cgccttatcg ataaggagat
     8821 agtgaatgag ttacgatcca atatttattg ctgggccaga tcgcagtggc acaacgttga
     8881 tttacgccct gctcgcctca catccaaata tttccatggt ccgacggaca aatatgtggc
     8941 ggtattttca caagcgatat ggagaactaa gccaaccgga aaattttgag cgttgcctta
     9001 acgacatggt gcgtttcaac cgcatgcgac aattacagcc cgatcccgag cgcattcgac
     9061 aagagttctg gcaaggcgaa ccaacttatg gtcgcttgtt tgccttgttt cacgaacatg
     9121 ttgcagaacg aagaggtaaa aagcgctggg gtgataaatc cttgcataca gaacgccatg
     9181 cagatcgggt attagcagaa tatcccaatg ccaaaatcat tcacattagt cgggatcctc
     9241 gcgacaggta cgcatccgtg cgaaagcgac atggtaaaga catgcccagg ctcggtgctg
     9301 caaccggtag atggttgttc tctatgagcg cggcgaggcg caacctgaag cgatatccaa
     9361 acaattacat gattatcaaa tttgaagaac tggcaagcca accagaagaa gtgctgcgca
     9421 aggtgtgtgc atttatcggt gaagagtaca cacctgctat gctgacaatg aaaggcgcag
     9481 ctgatcatcg cgataaaggt gggaacagct catttggcga gattaaacct ggtgtgatat
     9541 cgaaaagtcc gattggccgt ttccgaacgg tgctttcgaa gccagagatc gcgtttatac
     9601 agttgttcac cgcgcgtgaa atgaaagctt tgggttactc acgcgagcgt gtccgattct
     9661 cacctgttga attcttgaag tattacctat ggatattgcc aaaccatatg gctcgaatgt
     9721 ttggttcgat gatgctaact gcccgcggta ttcgaagggg tgagcgcatt ccagcccatc
     9781 gactactcga tttaccggta accacaccag gaggatatga tgcaaaagtt aaagagaccg
     9841 cccaaaatca aatctagccc attggctcag ccaagagttt tgattctctc gccctattac
     9901 cgtgtattga tcgccgggat cagcctggtg ctattgctct ctctggctat ctccacagcc
     9961 ctgtcccagg cgcccaacgc aacctggtac cccgtgcgct ccttcgaaat cgatgagctc
    10021 ggcatctcca accctgccgg cctggctttc agcccccagg acaacgcct
//
LOCUS       DBNGSX010000589         3856 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_617452, whole genome shotgun sequence.
ACCESSION   DBNGSX010000589 DBNGSX010000000
VERSION     DBNGSX010000589.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_617452"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..1736
                     /locus_tag="AC16CB_17100"
     CDS             252..1736
                     /locus_tag="AC16CB_17100"
                     /inference="COORDINATES: protein motif:HMM:NF013697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3344241.1"
                     /translation="MRKFNLIFTSLAVLAIMVAACQPAPAPTPERIVETVIVEKEGAT
                     VIETVVVTQEVEREVQVAGKTITFWSTETQPSRAEATKEIIRRFTEQSNIGVELVLTD
                     EDSLPNLMTAAVAAGTLPDVVFHPLDFTLGWYEQGILDAGAAAAVVDSLGRDTFAQGH
                     LDLVSVDGQVAAVPTDGWGQLIVYRADWFEAAGLDVPDTYEKIMAAAEALNDPGNNIY
                     GITAATDGGAVFTQQTFEQVALANGCQLVDADGNITLNSPECIEAVTFFDDLLTNYSA
                     PGVQDVVSTRATYFAGQAGMIIWSPFILDEMAGLRDNAFPSCPECTDDPAYLAKNSGI
                     VPAFSGPSGSPAQYGQISYMGITTNADVESAQQFLEFWFNDGYLDWLAVSVEGKFPMR
                     RGTSENPTQFIDGWKELETGVDRKATLGSIYSEDVINTLIEGASSFDRWGFPQGQGPL
                     VTAVYEALPVPRLLRDVLDNTLTPEAAVEEMQLEVEDLKAGLEQ"
     gene            1813..2769
                     /locus_tag="AC16CB_17105"
     CDS             1813..2769
                     /locus_tag="AC16CB_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018563722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3344242.1"
                     /translation="MARTTTKRLTLAQREARLAYLLLTPTFLVVALIVIFPVVWNLWL
                     SFKPISLGGLQAGDLLSTANLTLRNFERVLSGRNFWPVFQTTVVYSIFGTILSILLGL
                     AAAMVARDAFPGRQLFRGFLLVPYIAPIVAMTFLWRMIFNAQFGIFNEFTSNVLGWPR
                     TDYLSQRFWDMQLFGSTVQWPLALTMVILFEAWRYFPFAFLFILARLQALPDDLYEAA
                     AVDGAAPSQRFWYITLPQLRPVIATLFLLRFIWTFNKFDDIFLLNGGVAGTEVITIRI
                     FDLLFGQFQVGRAAALAVILAIFLGVFLFFYFRYVIVEEEAL"
     gene            2766..3614
                     /locus_tag="AC16CB_17110"
     CDS             2766..3614
                     /locus_tag="AC16CB_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018838309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3344243.1"
                     /translation="MTREQFETRLFKVLKWLGIIFFFLISFFPLFWMLVLSFRPLSQV
                     LQRPSALPSIDQILKAEPYLGVWVQQNLGKFIVNSLQVSLTTVVVTVVLGILGAYAAT
                     RLQFRGKTAFNAGILLIYMFPAMILAVPLFVVFTRLGLRNSLSGLVIVYLAQTLPVAL
                     YMLRSYFQTLPPELEEAALIDGCNRLQVIWRISIPLSVPAIASVSLYVFMIAWNEFLF
                     AILWLLERPEVWTISLGVQQLDKQEIPKTWLMAGSVIITIPIVVLFFFFERFLTKGLT
                     AGAVKG"
BASE COUNT          929 a          874 c         1011 g         1042 t
ORIGIN      
        1 ggttagggag ttaaaactgt tttctttcaa ctgcgcacgc ttgagggatt tcggttttta
       61 taacttaatc aaaacgtatc gggcaccaag gggctctagg ttgagtaatt gaaaggagga
      121 agtgagaatc aattcaaaag gaaataacag ttcataaccg gaaagcatcg tagcatcttt
      181 atgcaccgca gtattacagt tgggatgaag acaaattaag aattacaaca taacaataag
      241 gagcgagtaa gatgaggaag tttaatctaa tattcacatc acttgcggta ttggcaatca
      301 tggttgcagc ttgccaaccg gcaccagcgc ctactcccga acggattgta gagaccgtca
      361 ttgtggagaa ggaaggcgca acagtaattg agacggtggt agtcacacaa gaagttgagc
      421 gcgaagtaca ggtggctggg aagacaatca ccttttggag cacagaaacc caaccgtcac
      481 gcgctgaagc caccaaagag atcatacgcc gctttactga acaatccaat atcggggtgg
      541 agctagtgct caccgatgag gactcgctgc ctaacctaat gacagcagcg gtggcagccg
      601 gtacactgcc ggatgtagta ttccatccgc tggactttac acttggctgg tatgaacagg
      661 gcatcctgga tgcaggggcg gctgcagctg tggttgactc ccttggaaga gataccttcg
      721 cacaaggcca tctagacctg gtttcggttg atggtcaggt tgcggcagtg ccaaccgatg
      781 gctgggggca gctaatcgta taccgagcag actggtttga agctgccggg ttggatgtcc
      841 ccgataccta cgaaaagatt atggccgcag cagaggcgct caacgaccca ggcaacaaca
      901 tctacggcat tacagcagcg acggatggag gtgctgtgtt cacccagcaa acctttgagc
      961 aagttgcatt agccaatggg tgtcaactgg ttgacgcaga tggcaatatc accctaaatt
     1021 cacctgaatg catcgaagcg gtgaccttct tcgatgacct cttaacgaac tactctgcac
     1081 ccggtgtcca agatgtggtc agcactcgcg ccacctactt tgctggacag gcggggatga
     1141 tcatctggtc tccatttata ttggacgaga tggctggtct gcgagataat gccttccctt
     1201 cctgcccaga gtgcacggat gaccctgctt atctggcgaa gaacagcggc atcgtacccg
     1261 ctttctcagg ccctagtggc tcgccggcgc aatacggcca aatcagttat atgggtatca
     1321 ctacaaacgc agatgtagaa tccgcgcagc agttcctaga gttctggttc aatgatggtt
     1381 acctggactg gctggcggta tcggtagaag gtaagttccc aatgcgtagg ggcacttctg
     1441 agaatccaac tcaattcatc gatggctgga aagagttgga gaccggcgta gaccgaaagg
     1501 cgacgttggg ttcaatctac agcgaagacg tcatcaatac cttgatcgaa ggcgccagct
     1561 catttgatcg ctggggcttt ccgcagggtc agggtccctt agtcacagct gtatacgagg
     1621 ctctgcctgt tccgcgcctc ttgcgagatg tattagacaa taccctcaca ccagaagcag
     1681 cagtcgaaga gatgcaactg gaagtagagg atttgaaggc aggtcttgag caatagctga
     1741 acgttgtacc aaaaggggtg ccacgccttt aagggtgtgg cactcctgtt aacaatcttt
     1801 tggaggtaag caatggctag gacaacgaca aaaaggctaa ccctggccca aagggaagct
     1861 cgtctggcat atctgctact tactccaacg ttccttgtgg tggcgcttat cgtaattttt
     1921 ccggtagttt ggaatttgtg gctgagtttc aaaccgattt cgctcggagg attgcaagct
     1981 ggagacttgc tttcgacagc aaatctaacc ttgcggaatt ttgaacgggt gctaagcggg
     2041 cgaaacttct ggccagtgtt ccagacaacg gtggtctact ccatctttgg aacaattctc
     2101 tcgattcttc tcggtttagc tgccgcgatg gtggcacggg atgccttccc aggacgacaa
     2161 ctttttcgtg gattcctgct agtaccttat atcgccccga tcgtagcaat gacattcttg
     2221 tggcggatga tattcaacgc gcaatttggt attttcaatg aatttacatc taatgtattg
     2281 ggctggccgc gaactgatta tctaagccaa cgcttctggg atatgcaatt gtttggcagt
     2341 actgtccaat ggcctctagc cttgacaatg gtgatccttt ttgaggcatg gcgctatttc
     2401 ccgtttgctt tcttgttcat cctggcacgt ttacaagcct tgccagatga cctttatgaa
     2461 gcagccgccg tggacggggc tgctccttcg cagcgatttt ggtatataac cctgccgcaa
     2521 ttgcgaccgg tgatcgcgac ccttttcctg ctcagattta tttggacatt caataaattt
     2581 gacgatatat tcttgcttaa tgggggagtt gcaggaacgg aggtaatcac tatccggatc
     2641 ttcgacttgc tgtttggtca attccaggta gggagggcgg ctgcacttgc ggtgatattg
     2701 gcaattttcc taggtgtctt tctgtttttc tactttcgtt atgtgattgt tgaggaggaa
     2761 gcgctatgac tcgagagcaa tttgaaactc gcctcttcaa ggttctgaaa tggttgggga
     2821 ttatcttttt cttcctgatc tccttcttcc ccctgttttg gatgttggtg ctttcatttc
     2881 gaccgctctc acaggtgctt caaagaccct ccgcgctgcc gagtatcgat caaatcttaa
     2941 aggcagaacc ttacctcgga gtttgggttc agcaaaacct tggaaagttc atcgttaaca
     3001 gtttgcaagt atccctgacc acggtggtcg tgacggttgt cttgggcata ctgggtgcat
     3061 acgcagctac tcgattgcaa tttcggggaa aaacagcctt caacgcgggt atcttattga
     3121 tatatatgtt tcccgctatg atcctggctg tgcctctgtt tgtcgttttt actcgtctgg
     3181 gcttacgcaa ttcgctctcg ggtttggtga ttgtctacct agcacaaact ctacctgtgg
     3241 cactgtatat gctacggagt tatttccaaa ccctgcctcc agagttagaa gaagctgctc
     3301 tgatcgatgg gtgcaaccgg ttacaggtga tttggcggat ctctattcca ttatctgtgc
     3361 cggcaatcgc ctcggtctcg ctatatgtat tcatgattgc ctggaacgaa tttcttttcg
     3421 cgattttgtg gctccttgag agaccagagg tatggacgat ttccctaggt gtccaacaat
     3481 tagataagca ggaaatccct aaaacgtggt taatggcagg gtcggtgatc atcacgattc
     3541 cgattgtggt gctgttcttc tttttcgagc gatttctgac aaaaggactg acggcgggag
     3601 cagtaaaagg ttaatagaga attatttgag tggattaatt cacccacgct gaggggacaa
     3661 taagcatcgg gtaagaagga atatgttaaa atttctaccc caccgattat gtgcaatggt
     3721 ggggttttag cgagtattac gatccgcaag atgggagtgg ttgtggctcg ctggatttct
     3781 catggtcggc ggcattggcg attgaaatga ttgcaaataa tgtagctaga agaaggtgat
     3841 taggatgata gaccct
//
LOCUS       DBNGSX010000590        19660 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_896461, whole genome shotgun sequence.
ACCESSION   DBNGSX010000590 DBNGSX010000000
VERSION     DBNGSX010000590.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 19660)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19660)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19660
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_896461"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="AC16CB_17115"
     CDS             <1..262
                     /locus_tag="AC16CB_17115"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3344244.1"
                     /translation="VRRHSGAKNVRITFARDNGDFVGEIQDDGHGFALDSIGANGESP
                     HGYGLLSMQERVAQCCGQLEVISRPGSGTIIRVRFPIEENCA"
     gene            255..905
                     /locus_tag="AC16CB_17120"
     CDS             255..905
                     /locus_tag="AC16CB_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3344245.1"
                     /translation="MPDLIRVIIADDHTIVRSGVRLLLAAEPDIEVVGEALNGREAVA
                     LVEELQPDIVLMDITMPEMDGLEATRQIKAKYSQIQVLILTMHRSDEYFFEMLKAGAS
                     GYILKGAKTSELIDAVRAVCQGEVYLNPSMAQKLVQGYLNLVEWNTEDGLSLSPREKE
                     ILRLLAEGYSSKEIAEKLVVSSSTVHTHRSNLMSKLGLSTRRELMQYARQHGLTQD"
     gene            1060..1401
                     /locus_tag="AC16CB_17125"
     CDS             1060..1401
                     /locus_tag="AC16CB_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3344246.1"
                     /translation="MTSHQVFVIWKHPLFHESVRLLLKHPDVNLVGSSSDYTNAYEQI
                     QSLQPDTILMEAAEKLYSDEVVKLLESCPWSLRVMLISLGDNQLSMYYREQKTVGKAD
                     DLLHLIISHEL"
     gene            1514..2836
                     /locus_tag="AC16CB_17130"
     CDS             1514..2836
                     /locus_tag="AC16CB_17130"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3344247.1"
                     /translation="MQRKIIFQIGLITLLAFILAACAGTPGPEGPPGPAGPQGEPGPA
                     GTELTEEQLQAIETAGNLAGIQFPALDEVRRGCPACHVLVDNETGQYTLPYEAHERAE
                     ARGEEHPEVAPDGTSLAPTEEVSVTVCLQCHAAGTGDRAGKGVIAPLSLRDIVHPAHM
                     FSQWFKLHYGGNCFVCHNVDGEGAWELLTEKVDVNEKGVPNPENLPIPGAIPIGAEEA
                     ALSGSLSRGGKLYDKWWSEAGVDEPSADQPVWATQSTNTRSGSDTWRCKECHGWDYKG
                     AEGAYGTGSHFTGFPGVFGAQSKSLDEIVAILDGSSNPDHDFSAMGDSAMADLGAFLK
                     DGLVDYSPLIDAETKAANGGDVAHGEELYAGCVACHGEDGRAVNFGSDAEPEYVGTIA
                     LDNPWEFLHKVYAGQPGTAMPAALDGDWSMDDLLDLLAFSQTLPVTAP"
     gene            3123..3635
                     /locus_tag="AC16CB_17135"
     CDS             3123..3635
                     /locus_tag="AC16CB_17135"
                     /inference="COORDINATES: protein motif:HMM:NF025875.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR3344248.1"
                     /translation="MNRWLSFIALLIVAGVIGGVILTRTYVQAAPEQPLAYSHQTHVE
                     AGVQCLFCHSSALRLDIAGIPSVQKCMGCHTTVATDSEAVQVLSGYWERGEPIPWIRV
                     NQQPEFVYFSHQAHLGAGVNCETCHGDVGRMTVTRSAVKMDMGWCLDCHLKAPEDKIA
                     RLADCLTCHK"
     gene            3692..5908
                     /locus_tag="AC16CB_17140"
     CDS             3692..5908
                     /locus_tag="AC16CB_17140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR3344249.1"
                     /translation="MPKTLSRRNFLKIASGATVATGLAPAIRQMVIEPFVKSPEDTLP
                     GQATWYASTCRQCPAGCGIVVRTINGRAKKIEGNPLHPLNRGKLCARGQAALQVLYNP
                     DRLKNAVRQTGGRNSRKFEPLYWSEAIDLLKEKIASLSSADRLAFLGGLMPDHVYYLV
                     DRLLGALGAPPAVIYDLQSALEGRASASRLSQLFFGVPDLPIYDLAQSDVILSFGANL
                     FETWMSPVAQSIAYGTMRQGGMGRRGLLVQFEPRLSSTAASADKWIPLKPGAEGFVAL
                     AIGRIIVEERLGHVGSHRPHAVLYQNVDVRQMAAASEVPVETLRQLAQAFADADRPVA
                     IPGGYLAGQHNGISSMLAVQALNLVVAQVGREGGVFLSNPGPTVALRQKPAAHTFNEM
                     LDLIARMKAGEVDLLIVHSTNPMFELPAAAGFAEAISRVPFVVSFSQFVDETTVWANL
                     VLPDHTPLEAWGYQVPEPGADRPVISGQQPVVRPLYDTRATADVLLTLAARLGGKAAE
                     ALPWEDEVAYLEEITGELYGSSLGAYDARTSDGFWSLFRQYGGWWSEKEILREPELTE
                     VVQSPLPISEPRFDGDLQDYPFHLYPYSSIALSDGRGANLPWLQETPDPMTSVQWATW
                     VELNPETAGSLGVSDNDIVLVESPYGKLEAPVVVYPGIRPDVVAIPIGQGHKDYGRFA
                     QVASGSNPLALVAPVTDNEIGVLAWGTTRVRLKPTGRKHILARVESLDGKGRESLG"
     gene            5880..6605
                     /locus_tag="AC16CB_17145"
     CDS             5880..6605
                     /locus_tag="AC16CB_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3344250.1"
                     /translation="MAKVANLSAEGNHRWVLVVDLDRCTGCQACVVACHAENNVPVVG
                     EKQAAYGRWMHWIVIHRYWEGEYPNIKARFMPVMCQQCTEAPCEPVCPVFATYHNAEG
                     LNAQIYNRCIGTRFCGNACPYKTRTYNWFEPEFPEPLNEQLNPDVSVRTRGIMEKCTF
                     CIQRIRRTEIDAQAEGRAPADGEIQPACVQTCPTNALVFGDLGDPDSLASQMARSRRS
                     FRLLPELGTDPGVIYLKGGEIIV"
     gene            6598..8016
                     /gene="nrfD"
                     /locus_tag="AC16CB_17150"
     CDS             6598..8016
                     /gene="nrfD"
                     /locus_tag="AC16CB_17150"
                     /inference="COORDINATES: protein motif:HMM:NF015851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR3344251.1"
                     /translation="MSEPNNSTHEATQDSFYDERLLLAERTDEEINQAVLQPMFQSGK
                     GFWVLVTVLSLIVVWGMYAWGYLIYWGIGVSGKNRPVYWTLFITTFVFWVGISHAGTM
                     ISAVLRLLKADWRRPITRGAEAMTAFALMMAGLYPLIHLGRVWKFYWMIPYPNNRLMW
                     PNFQSPLMWDMVAIFTYLIGSSLYLYLALIPDMAMARDHTDSWRYKLYRVMALGWRGT
                     EAEWHKLHRALSIFSVAIIPIFLSVHSIVSWDFAVTIQPRWHSTIFAPYFVIGAVYSG
                     LSALASILVIVRRGMRLQEFLRAEHFDSLGKLVMVAGIAWFYFWFAGFIVEWYGNEPV
                     TRELIYQEVAGHLAPFFYLQMGANLIPVVLLVFKRIRTTPVLLLLTTLLVNVGMFTER
                     ALIVIGSLQRNEIPFNWGVYRPTWVEISIVAMTFAGFALLYTLFSRIIPFVPVWEIKE
                     GRLRYTVRRIGRLLVPTAAEIE"
     gene            8062..8586
                     /locus_tag="AC16CB_17155"
     CDS             8062..8586
                     /locus_tag="AC16CB_17155"
                     /inference="COORDINATES: protein motif:HMM:NF023249.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:electron acceptor oxidoreductase subunit
                     ActD"
                     /protein_id="HIR3344252.1"
                     /translation="MNETQALLALFYESTDTAESIDELHSLGIPDDKVVVITGVPYPE
                     RALGRHSEWLTLPYIVIAGAIGGMLFGFFLAVITPHLYRLDVGGHPPVGFPPAAVIVF
                     VFTMMATIVSTFLGVLWEMDFPHFGPVPYHKLVTDGHLAVLVEYPTDLERQVHQVFES
                     HHAHYVGEPERMTL"
     gene            8583..9089
                     /locus_tag="AC16CB_17160"
     CDS             8583..9089
                     /locus_tag="AC16CB_17160"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3344253.1"
                     /translation="MTSRRQRGLIFIAIILAPFIIGLLFTYEIIKIPFLTDMADQPSI
                     GYQEGPRILPPEGAVPVQGPSLIIQEFPVNPVESDEVSLQRGAILYDIHCQLCHGSQG
                     HGDGPISHYFDRTPQNLTAAQITGEFDGSVYLTILQGFGQMPSLAENLTPRERWDVIN
                     YVRTLPPQ"
     gene            9105..10157
                     /locus_tag="AC16CB_17165"
     CDS             9105..10157
                     /locus_tag="AC16CB_17165"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HIR3344254.1"
                     /translation="MRYYTHLVFLIIQALLVVGCAGPRSLPVGPTPIPTLIPATEPAT
                     LPGAAATPSFTILSYPARPPSTALGQPLYQTHCASCHGEDGHGVVPGARNFNDLDYIR
                     GETPAFFYSAVTEGRGEMPAFLNELTSDERWDVVFYTWRFSTNVETLALGKSIYDTNC
                     SACHGIDGVGKVLGAADFTDLRVVDARAPRDFYLTVTQGIGSMPAWQGRLSQEERWAV
                     IDYLRTFSYDATLPGEVAATPTAQVAAPEIACTTTYVSNTNPFAWDDAAASEAGELIY
                     SQSCSICHSVDGSGAMPGAPDLTTAEKQDELRSEDGEFPCIMAEGQGAMPGWKETLSA
                     DQIWQVLTYIGSLTAP"
     gene            10189..12570
                     /locus_tag="AC16CB_17170"
     CDS             10189..12570
                     /locus_tag="AC16CB_17170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR3344255.1"
                     /translation="MKSSRRDFLKLSGAIGATAIGASLVRPFFEATAQTSTLPYMQQW
                     ISTICLMCPGGCGILVRTINGRAVKIDGNPLHPVNTGKVCPKANAALQVLYDPDRLRG
                     PMVQKGERGSDGWEAISWDEATQLVAERLADIRVNPGPQAMAFMSGRVRGQMGGLIDR
                     FMAAYGSPNHIRHDSLSIDGERIGNELTFGHREQLAYDWDNCNYVLSFGRAFLEASRP
                     TVRQIRGFSAMRRDRPNRRAKFVQIDTRFSITAAKADEWVPIRTGTDGALALGIAHIL
                     IAEELYDRRFVSEHTHGFEDWTDENGVAHEGFRSMVMREYHPVQVESITGVSADTIIR
                     LAREFANAQPGVACGGRGAVMQTNGVFNWMAIQALNALVGSIEVPGGVLVQNRPPYSP
                     WPDLPADPVADAGLAMTRFDGAGTRAYPLAHSVYQDLPERILAGEPYPLGALFIYYTN
                     PLFSSPDVKRFYEAIQKVPFVVSFSPFLDDSSAYADLILPDHSFLERLQDDEIEPSLG
                     YAVLGLRQPVVAPLFDTRDTGDVLIQIARAMGGSMEAAFPWSDFSQVIQSRLSGLWAF
                     ESGSIRSTNFSHFWDELISQSVWSDAPYAFGQWGRVFNTASGKFEFFSQKLYEKLDGL
                     RGEQTWGEWLSELGIDASGDMAFMPHYESPRHIGRESEYPFFLNTYKLMTHAEGRGAN
                     TPWLQEKLGMHIKKSWDAWVEINPRVAERLGIEDDDWVWIESPIGKIKLRAVLYPGAR
                     PDTLNIPFEQGHKHYGRWAKERGENPNWVVANESDQLAGIAAWFSTRVKIYKA"
     gene            12579..13208
                     /locus_tag="AC16CB_17175"
     CDS             12579..13208
                     /locus_tag="AC16CB_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3344256.1"
                     /translation="MPHWGMVIDLDRCVACQACSAACRAENNVSFAGPEETALGRAIY
                     WQEVLAEVDGEYPNVRGTFIPRPCMHCENPPCVRVCPVGATYQNEEGLVLQRYERCIG
                     CRYCMVACPYAVRYFNWRAPEWAEGMERYQNPDVPIRPKGVVEKCTFCTHRLPVPACN
                     QACPGNARFFGDLEDPDSVVSQLARSPRAFRLLEDLGTEPKVYYLREGA"
     gene            13210..14532
                     /gene="nrfD"
                     /locus_tag="AC16CB_17180"
     CDS             13210..14532
                     /gene="nrfD"
                     /locus_tag="AC16CB_17180"
                     /inference="COORDINATES: protein motif:HMM:NF015851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR3344257.1"
                     /translation="MNKDDKEAVLFKPVEKTGRGFYLVVAGLVLILAWAAYAYSRQIV
                     VGLDVTGLNRPVYWGLYITNFVFFIGISHAGTLVSAILRVFGAEWRRPITRAAEAITV
                     FALVLGTGNIMIDMGRPDRLLNIIRYGRFQSPLVWDVTSLTVYLTASLTYLYLPLIPD
                     LAILRDRPGLSPWRRWLYTKLSIGWQGTEEQKHRLERLIGIMAVLIIPIAVSVHTVVS
                     WVFAMTVQPMWHSTIFGPYFVIGAIFSGIATLIIVMVILRRAFNLYAYITDQHFSNLG
                     LLLMIFAFLWAYFTFSEYLTEFYGNEPAGMRVLFAKLVEEFSLMFWAMWLFNFILPFI
                     LLSNRRTRTVTGTVFTSILVNVGMWLERYNIVVPTLTRPRLPSPITSYTPTWVEWSVL
                     VGSFAVFAMLYVLFSKLFPIVSIWEVREGEEIAAGGVVAGHHAEAGAD"
     gene            14535..15485
                     /locus_tag="AC16CB_17185"
     CDS             14535..15485
                     /locus_tag="AC16CB_17185"
                     /inference="COORDINATES: protein motif:HMM:NF014651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HIR3344258.1"
                     /translation="MENELLTRAEGYAYLASMFRRPETATAAEPGSTSLDIVQAYRRL
                     FLGPGRPIVSPYESVHLEGQLMGASAARVVESYAEANLRLSPDEHELPDHVSIELAFM
                     AYLAEQEQCDPDQAEVWRERQRCFLLDHLGRWLPLFCQKVEASRMHPFYSEAAQITRQ
                     LVGSDMAEFLPSVRKRRYANICLEVDGARCTLCSLCTDSCPKDALHVVSTPAQLSLLF
                     DSSHCNGCRVCLRLCPEQAINLKRNPPQDFPVLAAKQVVAVADRAVCPECHRPHVAEP
                     WLDRLAERMGGGAFALHTLKLCPFCKVAVGNSLPVSSGIP"
     gene            15502..16881
                     /locus_tag="AC16CB_17190"
     CDS             15502..16881
                     /locus_tag="AC16CB_17190"
                     /inference="COORDINATES: protein motif:HMM:NF024827.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HIR3344259.1"
                     /translation="MRKYITLIIIIFIGLLLSPAVFDVRGQESETEDTPQNDYVGSVT
                     CSRCHANTYETFKQTWHPNMLRPATDDNILGDFNTQDPDLDFTREEVDWVLGGQYKQR
                     YLTEEDGQLFVLLSEWNVETREWSAYVTGEWVPYEGQIWRKRPYAPYCAGCHTTGFNP
                     VTQTWVEPGVGCEACHGPGGDHVASTGNRTKIINPANLEFRDQVEICAQCHSRGEDPS
                     EQFPYPVGYYPGGPAKLGETFILTTDPNDFWPNGTAKRHHMQYQDWQIGEHKDSVSCI
                     FCHTSHSVGETDHQTRMVGNDRCVICHEGQTDLAAHIPFMASVADQVNCTDCHMPQIS
                     KLVPTDFEILSHTFSPPDPAVSIAYGGFEQMPNACNLCHTDKSPEWAAGVLGQEIPTA
                     DATPVPQPTPMPLPTSVFAQAGLFASEEVNPPDTQAGSGALTLWWLGLLAVILVFAFL
                     WARRRMNSE"
     gene            16878..18071
                     /locus_tag="AC16CB_17195"
     CDS             16878..18071
                     /locus_tag="AC16CB_17195"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3344260.1"
                     /translation="MKINSLFLTLLIFFASLLLVGCLGEADLPALESRPVPPLAPPAV
                     DPTVAALPRQDIFTGAQVSVSNQQAPLIPHDMIGRTDCLMCHKQGVSNAPRIPDGHRG
                     LESNTCQTCHASLASLELSGAEMYQRICTRCHGEDGEGGAGPALNTKSYLRNVTDEEI
                     RAAIVRGLGESQMLAWGDLGLLTDRQIDELVEMIRAWEPTAPEVAIIAHQPPSAAQGD
                     PGRGSELFAQFCSGCHGIEGETVLVDGFVLSQLVSQLEDDVISKQINDGTEEMPSFHA
                     LLTRDDINDLLAFMRETFEGGGETSMTSLPSFTQEILPLFNQKCVFCHGASGGWQSGS
                     YDEVMNSGMNAPVVIPGDAEGSLLVQKLRGTQAEGAPMPPGTPLSETEIDLVVRWVEA
                     GAPDN"
     gene            complement(18127..19248)
                     /locus_tag="AC16CB_17200"
     CDS             complement(18127..19248)
                     /locus_tag="AC16CB_17200"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR3344261.1"
                     /translation="MEFDLVFEGGGAKGLAFVGALQTFERRGHTPRRVIGTSAGSILA
                     VLIAAGYNAAECMAALNEKLPDGSSRFTSFLETPQIDEKIEINNSLRYWLKTELDNPM
                     IPNMIEPVVDQLVMGMVQKDLARHLISLLVWGGWYSGNALMGYLHEKLDAGGRNLGQS
                     TLLEFHHSTGRDLSVVASDVTGREMLVLNHRTAPDCPTTWAVRMSMSCPFVWHEVIWR
                     KEWGMYRGRDLSGHKVVDGGLLSNFPINLLVSTDDTIEEIMGRDSASEQVIGMLLDDS
                     MPVPGAEDTQKSESDAPSFLERMDLLAEMMLRIQGMAETVLGAHDKTIIDAHQHFVCQ
                     LPAKGYGILEFDLSAERMEPILKAGEATMEAHFQKYYDR"
     gene            19405..>19660
                     /locus_tag="AC16CB_17205"
     CDS             19405..>19660
                     /locus_tag="AC16CB_17205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344262.1"
                     /translation="MLAALKSKMMRPLTNRLARPVDAVAHERLRLRLLFAVAILKLFN
                     FIGGAWDIQWHVAIGRDSLFIPPHLLVIFAFVAGLALVLAL"
BASE COUNT         4306 a         5109 c         5400 g         4845 t
ORIGIN      
        1 cgtaagacgt cattctggag ctaaaaatgt gcgcatcact ttcgcacggg ataatggcga
       61 ttttgtgggc gaaattcaag acgatggaca tggctttgcc ctagattcca ttggcgctaa
      121 cggggaaagc ccacatggct atggtctact aagcatgcaa gagcgcgttg ctcaatgttg
      181 tgggcaatta gaggttatat ccaggcctgg cagcggtaca ataatacgag ttcgtttccc
      241 tattgaggag aattgtgcct gatctaattc gcgtgattat tgcagatgac cacaccatcg
      301 ttcggtcggg tgtgcgcctc ctcctagcag cagagcctga tattgaggtg gttggcgagg
      361 cgctcaacgg tcgtgaagct gtcgccttgg tggaagagct tcaacccgat attgtcttga
      421 tggatatcac catgcctgag atggatggtc tggaggctac acgccaaata aaagctaagt
      481 attcccaaat tcaagtgtta atattgacga tgcatcgctc agatgagtat ttctttgaaa
      541 tgctcaaagc aggtgcctcc ggatacatac tgaaaggcgc taaaaccagc gagctcatcg
      601 atgccgtgcg ggctgtgtgt cagggtgaag tctacctgaa tcccagcatg gcccagaaat
      661 tagtccaggg atatctaaac ctggtggaat ggaatactga ggatggccta tcgctaagcc
      721 ctcgcgagaa agaaattcta cgcctgcttg cagaaggata ttcgagtaaa gagatcgctg
      781 aaaagctggt tgtcagttcc agcacagtgc acacacaccg cagtaattta atgagcaagc
      841 ttggactgag cacccgtcgt gagttgatgc aatatgcccg ccaacatggc ttgacgcagg
      901 attagtctgt cacatcaaaa ctgggcaaaa acgccagatt atccaaaaaa tcgccagaac
      961 agttggcgat tttttcattt agaaaccgca ctttgctcga tacccaaatg agaaattttt
     1021 cactatattt taatatagtt ctcaggcagc ggagattaga tgacgagtca tcaggtattt
     1081 gtgatatgga aacatccgct ttttcatgag tcggtccgtt tgctcttgaa gcatccggat
     1141 gtaaatctgg tgggctcgtc ctcggattat acaaatgctt atgagcaaat ccagagcctg
     1201 cagcccgata cgatcctcat ggaggcggcg gaaaaactgt attccgatga agtggtgaag
     1261 ctgcttgaat cctgtccatg gagtttacgg gtgatgctga tcagtttggg cgataaccag
     1321 ttgagtatgt attaccgcga acagaaaacg gtcggaaaag cagatgattt gctccatttg
     1381 attatcagtc atgagttata aggagggaga aaatgtcata aatgccatgt tgttgttcag
     1441 ttacttatca ctgacacgaa ccaatccatc atagttagga cagatcgaca agtaatctcg
     1501 aggagtgtgc agaatgcaaa gaaaaattat attccagatt ggtttgataa ccttgctggc
     1561 attcatcctg gcagcctgtg cggggacacc cggacctgaa ggtcctccgg gaccagcagg
     1621 accgcaagga gaaccagggc ctgctggtac agaattgacc gaggagcagc ttcaggccat
     1681 cgaaactgcg ggcaatttag ctggcatcca gtttccagct ctagatgagg tgcgccgagg
     1741 ctgccctgct tgccatgttc ttgttgataa cgagactggt caatataccc tcccttacga
     1801 ggcgcatgaa agggctgaag cccgcgggga ggagcatcca gaagtcgcac ctgatggtac
     1861 atcccttgcg ccaacagaag aggtaagtgt gacggtctgc ctgcaatgcc acgcagcggg
     1921 cacgggtgac cgggctggaa agggtgtcat cgcgccgcta tcgttgcgcg atatcgtcca
     1981 tcccgcccat atgttcagcc aatggttcaa gctgcactat ggtggcaact gctttgtttg
     2041 ccacaacgtt gacggcgagg gggcgtggga gcttcttact gaaaaagttg acgtcaatga
     2101 gaagggcgta ccaaacccgg agaatctgcc gatcccgggt gcaatcccaa ttggggcaga
     2161 agaagctgcg ctgtcaggtt cgctctctcg gggcggcaag ctatatgaca agtggtggag
     2221 tgaagccgga gtagatgagc caagtgcaga ccaacctgtg tgggccactc aatccaccaa
     2281 cacccgtagc ggttctgaca catggcgttg caaggaatgt cacggttggg actacaaagg
     2341 agcagagggc gcttatggca ccggttccca cttcactggc ttcccaggtg tattcggtgc
     2401 acaatcgaaa tccttggatg agatagtagc gatcctagat gggagttcca atcctgatca
     2461 cgacttttcg gcgatgggcg atagtgccat ggctgatctt ggtgctttcc ttaaggatgg
     2521 ccttgttgac tattcacccc tgattgacgc tgagacaaaa gcggccaacg gtggtgatgt
     2581 cgcacatggt gaggagctct atgctggatg cgttgcctgc cacggtgaag atggtcgtgc
     2641 cgtaaatttt ggaagcgatg ccgaaccgga gtacgtcggc acgatcgctt tagacaaccc
     2701 ctgggagttc ctccacaagg tgtacgctgg tcaacccggt acagctatgc cagctgctct
     2761 ggatggagat tggagcatgg atgatttgct agatttgctg gctttctctc aaaccttacc
     2821 tgttacggca ccttgagttt aagcaagata tattccacaa ttgggccggg gttgaatatc
     2881 tcggcccaat tcctgaatag agaaatgaat atatctcgac gcgaaaacat caagctatcc
     2941 acgtccattg ctaattttgt ggacgactta gatgaaaagc ctaacctggc acgctcatgc
     3001 ttcgttaagc agtgcccgcg gaaacccgaa agtcgcgcaa cagtacgaga ggatatccgg
     3061 ttcgttgcta tgaaagccgc acggcaaact caggtgcatt ttaccaggga aaatttgatc
     3121 acatgaatcg ttggctttct tttattgcct tgcttattgt agccggagtc atcggcggag
     3181 taattctcac tcgcacctac gtccaggccg cgccggagca gcctctggcc tatagtcacc
     3241 agactcatgt ggaggctggc gtgcagtgtt tgttctgcca ttctagtgca cttcgtctcg
     3301 atatagcggg cataccctcc gttcagaaat gcatgggctg ccataccacc gttgccacag
     3361 atagtgaggc ggtccaggtc ctatcaggtt actgggagcg cggcgagccc attccgtgga
     3421 tacgtgttaa tcagcaacct gaatttgtct acttcagcca tcaagcccac ctgggggctg
     3481 gcgtcaattg tgagacttgc catggcgatg ttggccgcat gacggtcact cgatcggcag
     3541 tgaagatgga tatgggttgg tgtctagact gccacttaaa agcccccgaa gataaaatcg
     3601 cccgtttggc agactgcctg acgtgtcaca aatgaaatct aaaaggtgag acgaaaacaa
     3661 aatcacattg aattttacac tctgaaaaac tatgcccaaa acactctcgc gcagaaactt
     3721 tcttaagatt gcctccggag ctacagttgc caccggctta gcaccagcta ttcgccaaat
     3781 ggttatcgag cccttcgtga agtcgcctga agatacgctt cccggacaag ccacctggta
     3841 tgccagtacc tgccgccagt gtcctgcggg ttgtggtatc gtggtgcgga ctatcaatgg
     3901 gcgggcgaag aagattgaag gcaatccctt acatcccctc aatcgaggaa agctgtgcgc
     3961 ccgaggacag gcagctttgc aggtgcttta caaccccgat cggcttaaaa acgccgttcg
     4021 ccagacggga ggccgcaatt cacgcaagtt tgagccactc tactggtcgg aggcgattga
     4081 cttgttgaaa gaaaagattg catccctcag tagtgcggac cggttggctt tcctgggcgg
     4141 gctgatgcct gaccatgtgt actatttggt tgaccgtctg ctcggtgccc ttggcgcccc
     4201 ccctgcggtg atctatgact tgcaatcggc gctggaaggc cgtgcctcag cctccaggct
     4261 gtcgcaacta ttctttggtg taccggatct gcctatttat gatctagccc agtctgatgt
     4321 gattctttcc tttggcgcga atctctttga aacttggatg tcaccagttg cccaaagcat
     4381 tgcgtatggg accatgcgcc aaggagggat gggcaggcgg ggattgctcg tgcagtttga
     4441 gccgcgcctt tcgtcgacgg cagcgtcagc tgataaatgg attccgttga agcctggagc
     4501 tgaggggttt gtcgcccttg ccatcgggcg catcatcgtt gaggaacgac tcggtcatgt
     4561 tggcagtcat cgacctcatg ccgtgttgta ccagaatgtg gatgtgcgcc agatggccgc
     4621 tgctagtgaa gtgccggttg aaacactgcg tcagcttgca caggcttttg ccgatgccga
     4681 tcgacctgtg gctatccctg gtggttactt ggctggacag cacaacggta tttccagcat
     4741 gctggccgta caggcgctta atctagtagt agcacaagtt ggtcgggagg gtggagtatt
     4801 tctttcaaac ccgggcccaa cagtggcttt gcgacaaaaa cctgcggctc atacgttcaa
     4861 cgagatgctg gatctgatcg cccgaatgaa agccggggaa gttgacctgt tgattgtcca
     4921 tagcacgaat cctatgtttg agctaccagc tgcagccggt ttcgcagaag ctatctctcg
     4981 tgtgcctttt gttgtttcct tcagccagtt tgttgacgaa acaacagtat gggcgaacct
     5041 ggtcttgcct gaccacactc ccttggaggc ctggggctat caggtgcctg agccaggcgc
     5101 agaccgtcct gttattagcg ggcagcaacc ggtggtgcgt ccactttatg atactcgcgc
     5161 caccgcggat gttctgctta ccctggctgc acgcctcggt ggtaaggcag cagaggcact
     5221 tccttgggaa gatgaggtag cttatttgga agaaatcacg ggtgaattgt acggttctag
     5281 cctcggcgca tacgatgctc gaacctcgga tggcttctgg tcactcttcc gccaatatgg
     5341 gggctggtgg tcagaaaagg agatcctccg tgagcccgaa ctaacagagg tagtgcaaag
     5401 ccctctacct atatccgagc cgcgatttga cggtgacctg caagattatc ctttccacct
     5461 gtatccctat tcttcaatag ctctttcgga tgggcgtggc gcaaatttgc catggcttca
     5521 ggaaacgccc gacccgatga cgtcagtgca atgggcaacc tgggtggaat taaatcctga
     5581 aactgccggc tcgctgggtg tgagtgataa cgatatcgtg ctagttgaat cgccgtacgg
     5641 taagttggag gcaccggttg ttgtctatcc ggggattcgt ccagacgtgg ttgccatccc
     5701 gatcggtcag ggacacaaag attatggacg ttttgctcag gtggccagtg gtagcaaccc
     5761 gttggccttg gtagctcccg tcacagacaa tgaaataggc gtattggcct ggggaaccac
     5821 acgcgtacgc ctgaagccaa ccggacgcaa gcatatttta gcgagagtgg agagtttaga
     5881 tggcaaaggt cgcgaatctc tcggctgagg gtaatcatcg atgggtgctg gtggtcgatc
     5941 tggatcgctg tactggctgc caagcgtgcg tcgttgcttg ccatgcagag aacaatgtac
     6001 cggtggtcgg tgagaagcaa gcagcctatg ggcgttggat gcactggatc gtcattcacc
     6061 gctactggga gggtgagtac ccgaatataa aggcacgttt catgcctgtg atgtgccagc
     6121 agtgcacaga ggccccctgt gagccggtct gcccagtgtt tgcgacctat cacaacgctg
     6181 agggcttgaa cgcgcaaatc tacaaccgct gtattggcac gcggttctgt ggcaatgctt
     6241 gtccgtataa aactcgaact tataactggt ttgaaccgga gttccccgaa ccgctgaacg
     6301 agcaactcaa cccggatgtg tccgttcgga cgcgtggcat catggagaaa tgcacctttt
     6361 gtatccagcg catccgccga acagagatcg atgcgcaagc ggagggacgc gcacctgcgg
     6421 acggtgaaat tcaacctgct tgcgtacaga cttgtcccac caatgcgctg gtttttggcg
     6481 atctgggcga tccagacagc ctagccagcc aaatggcacg cagccgccgc tcttttcgtc
     6541 ttttgccaga attgggtacc gatccgggag ttatctacct caaaggaggc gagataattg
     6601 tctgagccta ataattcgac acacgaagca acacaagact cgttttacga cgagcgcttg
     6661 ctcctggccg aaagaactga tgaagagatt aaccaggccg tactccagcc aatgtttcaa
     6721 agtggcaagg gtttctgggt gctcgtgaca gtcttaagtt tgatcgttgt ctggggcatg
     6781 tatgcatggg gttatcttat ctattggggc attggcgtat ctggtaaaaa ccgaccagtc
     6841 tactggactc tctttatcac cacattcgtc ttttgggtcg gcatcagcca tgctggcacg
     6901 atgatttcgg ctgtattacg cttgctaaaa gccgattggc ggcggccaat tacccggggt
     6961 gccgaagcta tgaccgcttt tgcgttaatg atggctgggt tatatcccct tattcacctg
     7021 ggccgggtat ggaaattcta ctggatgatc ccttacccca ataatcgctt gatgtggcca
     7081 aactttcaat ccccgttgat gtgggatatg gtcgccatct ttacctattt aattggctca
     7141 tccctgtacc tatatctggc gctgatccca gatatggcta tggcgcgcga tcacaccgat
     7201 agctggcgct ataaacttta ccgtgtgatg gcgctcggtt ggcgtggcac ggaagccgaa
     7261 tggcacaagc tgcatcgcgc attgagtatc ttttcggtcg ccattatccc aatcttcctc
     7321 tccgttcact cgattgtgtc gtgggatttt gcagtaacca tccagccgcg ctggcacagt
     7381 accatctttg caccctactt cgtcatcggt gcggtttatt ccggtctctc tgccctagcc
     7441 agcatcctgg tgatcgtgcg gcgtggaatg cgactgcagg aattcctgcg cgctgaacat
     7501 tttgattcat tgggcaagct ggtcatggtg gcaggaatag cctggtttta tttctggttt
     7561 gctgggttta tcgttgagtg gtatggcaac gagccggtta cccgcgaatt gatctaccag
     7621 gaagtggctg ggcatctagc gccgtttttc tatttgcaaa tgggtgccaa tctgattcca
     7681 gtcgtccttt tggtttttaa acggatccgt actaccccgg tgttgctgct gttaaccacc
     7741 ttgctggtca acgttggtat gtttaccgag cgggcgctca tcgtaattgg aagtctgcaa
     7801 cgtaacgaga tacccttcaa ttggggtgtc tatcgtccga cttgggttga gattagcatc
     7861 gttgccatga cctttgccgg ctttgcgctg ctgtacacgc tgttttcccg tatcatccca
     7921 ttcgttccag tgtgggagat caaggaagga cgcttgcgtt acaccgtaag gcgaatcggg
     7981 cggttgttgg tgccgacggc agcggagatt gaatgagaag aatccaatca catgatagct
     8041 ggtggagatg gttgacgaca tatgaatgaa acacaggctt tattagccct cttttacgag
     8101 agcactgata cggctgaatc aatcgatgag ttgcacagcc taggtatccc cgacgacaaa
     8161 gtcgtggtga tcactggcgt gccatatcct gaacgcgcct taggacggca tagtgagtgg
     8221 ctgactctcc cctatatcgt gattgctgga gcgatagggg gaatgctgtt tggcttcttt
     8281 ctggcggtta tcacgcccca cctttaccgc ctggatgtag gtggccatcc cccagttggc
     8341 ttcccgccgg ctgcagtgat cgtctttgtt tttaccatga tggctaccat cgtctcaaca
     8401 tttctgggtg tgctttggga gatggatttc ccacattttg ggccggttcc gtatcataaa
     8461 cttgttacag atggtcacct ggctgttttg gtggaatatc caactgatct ggagcgtcag
     8521 gtacaccagg tatttgaatc ccatcatgca cactatgttg gcgagccaga aaggatgacg
     8581 ctatgacctc gcgccggcaa cggggcttga tttttatcgc tataattctt gcgccattca
     8641 tcatcggctt gttattcaca tacgagatta tcaagatccc gtttctcacc gatatggctg
     8701 atcaaccttc aataggttac caggaaggac cgcgtatact gcctcctgag ggggcggtgc
     8761 ctgtgcaggg gccttcctta attatccagg agttccccgt aaacccagtg gaatccgacg
     8821 aggtttctct ccagcgcggg gctatcctat atgatatcca ctgccaactc tgtcatggtt
     8881 cacaggggca cggcgatgga ccaatctccc actattttga ccgaacgccg cagaacttaa
     8941 ccgccgcaca gattactggc gagtttgacg gctcggtcta cttgaccatt ctgcagggat
     9001 tcggccaaat gccttcgctg gctgaaaacc tgacaccacg cgagcgctgg gatgttatta
     9061 attatgtccg aactttgccg ccgcaataat gagaggtcac cttaatgaga tactacacac
     9121 acctggtatt tcttatcata caggcgttgc tcgtggtagg ttgtgccggt ccgcgcagct
     9181 tacctgtcgg tcccactcct atacctacgc ttatcccggc aaccgaacca gctaccctcc
     9241 caggcgccgc tgccacgcca tccttcacca tccttagtta tccggctcgg ccaccctcaa
     9301 ctgctctagg ccagccgctc taccagactc attgtgcaag ttgccatggc gaagacgggc
     9361 atggcgttgt gcctggggca cgtaacttca atgatctgga ttacattcgt ggcgagaccc
     9421 ccgccttctt ctactcagca gtcacagagg gacgcggcga aatgccagca tttctgaatg
     9481 agctgacatc tgatgaacgg tgggatgtgg ttttctacac ctggcgtttt tccaccaacg
     9541 ttgagaccct cgctcttggt aagagtatct atgataccaa ctgcagtgcc tgccatggca
     9601 tcgacggtgt gggtaaagta ttgggcgcgg ctgacttcac tgacctgcga gtggtggacg
     9661 ctcgggctcc ccgcgatttc tacttgaccg ttacacaggg catagggtcc atgcctgcct
     9721 ggcaagggcg tctctctcaa gaagaacgct gggcagtgat cgactacctg cgaacattct
     9781 catatgacgc tactctgccg ggagaagtcg ccgcaacgcc tacagcacaa gtagccgccc
     9841 ctgagatcgc ctgcactacc acgtatgtat ccaatacaaa tcccttcgcc tgggacgatg
     9901 cagccgcaag tgaagccggt gagttaatct acagccagag ctgtagtatt tgtcatagtg
     9961 tagacggttc gggtgccatg ccaggtgcac ctgacttaac tacagctgaa aagcaagacg
    10021 aattacgctc agaagatggg gaatttccgt gcattatggc tgaaggtcag ggcgcgatgc
    10081 ccggttggaa ggagacccta agcgccgacc aaatctggca ggtgcttacc tatattggct
    10141 cactcacagc cccatgacat cctttggatc gaccatatga gttacaaaat gaaatcctca
    10201 cgtcgtgact ttctgaaact ttcaggcgct atcggcgcaa ccgccatagg agcgagcctg
    10261 gtgcgcccgt tcttcgaagc cactgcccaa accagcaccc tcccttacat gcagcagtgg
    10321 atatcaacga tctgcttaat gtgcccaggt gggtgcggaa ttttggtccg gacaatcaat
    10381 ggcagggcgg tcaagattga tggcaatcca cttcatcctg tcaacacagg aaaagtatgc
    10441 cccaaagcaa acgccgcttt gcaggtgctt tacgatccag accggttaag gggcccgatg
    10501 gtgcaaaagg gagagcgcgg aagtgacggt tgggaggcaa tcagttggga cgaagcgacc
    10561 caattggtcg ccgagcgcct ggcagatatc cgcgtgaatc ctggacctca agccatggcg
    10621 ttcatgtctg gccgcgtgcg gggccagatg ggaggcctga ttgaccgctt catggctgcc
    10681 tacggctcgc ccaatcacat ccgccatgat agcctcagta tcgatgggga gcgcattggc
    10741 aatgagctaa cttttggtca ccgcgagcaa ttggcttatg actgggacaa ctgcaactat
    10801 gtgctcagct tcgggcgcgc ttttctggaa gcctcccggc caaccgtgcg ccagatacgt
    10861 ggattcagcg cgatgcggcg cgatcgaccg aatcgacgcg ctaaatttgt gcagatcgac
    10921 acgcgctttt cgattacagc tgccaaggca gacgagtggg tgccaattcg cactggtact
    10981 gatggcgcac tggcacttgg aattgcccac atcctcatcg cagaggagct ctatgacagg
    11041 cgttttgttt cggagcacac acacggcttc gaagattgga ccgatgaaaa tggcgtcgct
    11101 catgagggct tccgctccat ggttatgcga gagtaccatc ccgtacaggt ggaatcgatt
    11161 acgggtgttt cggccgatac gatcatccgc ttagcacgcg aatttgccaa cgcgcagcct
    11221 ggcgtggcgt gcggagggcg tggtgctgtg atgcagacca atggtgtttt caactggatg
    11281 gcgatccagg cgctgaatgc cctggtcggt agcatcgaag ttcctggggg agtgctggtg
    11341 caaaatcgtc cgccttattc accttggcct gacctgcccg cagatccagt tgctgatgca
    11401 gggcttgcca tgacgcgctt tgatggtgcc ggtacgcgtg cataccccct ggcgcacagc
    11461 gtttatcaag acttgcctga acgcatcctg gctggcgagc cctatccgct tggcgcgctt
    11521 tttatttact acaccaaccc gcttttctcc tcgccggatg tcaagcgatt ttacgaagct
    11581 attcagaagg tgccgtttgt tgtttccttt tcgcctttcc tcgacgatag cagcgcttat
    11641 gcggacttga tcttacctga tcattccttc ttggaacggc tgcaggatga cgagatcgag
    11701 cctagcttag ggtatgctgt tttgggtttg cgacagcccg tggttgcacc attgtttgat
    11761 acccgcgata ccggcgatgt gctcatacaa attgcacgcg ccatgggcgg gtctatggag
    11821 gcggcttttc cctggtctga tttttctcag gtcatccaaa gccgtctcag cggcttgtgg
    11881 gccttcgaaa gcgggtcgat ccggtcgacg aatttctccc acttctggga tgagctgatc
    11941 agccagagtg tctggtcgga tgcgccttac gctttcgggc agtggggtcg cgtcttcaat
    12001 accgcttccg gtaaatttga attcttttcg cagaaattat acgaaaaact ggatgggttg
    12061 cgtggagagc aaacctgggg cgagtggtta agtgagttag gaatcgatgc cagcggagac
    12121 atggccttca tgccccacta tgagtccccc aggcacatcg ggagagagag tgagtatccc
    12181 ttcttcctta atacctataa gctgatgaca catgccgagg ggcggggagc aaataccccc
    12241 tggctacaag agaaactcgg catgcacatc aagaaaagct gggacgcgtg ggttgagatt
    12301 aatcctaggg tagcggagcg gctaggcata gaggatgatg attgggtatg gatcgagtct
    12361 cccatcggta aaatcaagct cagggccgtc ctctatccag gtgcccgtcc agatacgtta
    12421 aacataccct tcgaacaggg tcataaacac tacggtcgtt gggccaagga gcgcggagaa
    12481 aacccaaatt gggtggttgc gaatgaaagc gaccaactgg cgggcattgc ggcctggttt
    12541 tcgacgcgcg tgaagatcta caaagcttag gagtaaatat gcctcattgg ggaatggtga
    12601 ttgacttgga tcggtgtgtg gcctgccagg catgctcggc tgcctgccgg gcagagaaca
    12661 atgtttcctt tgccggacct gaagaaacag ctctcgggcg ggcaatttat tggcaagaag
    12721 tgcttgctga ggtggatggc gaatatccca atgtgcgcgg aacgtttatc cctcgccctt
    12781 gtatgcactg tgaaaacccg ccttgtgttc gcgtgtgccc ggtaggggct acctaccaaa
    12841 atgaagaggg attggtattg cagcgctacg aacgttgcat tggatgccgc tactgtatgg
    12901 tggcctgtcc ttatgcggtg cgctacttta actggcgtgc ccccgaatgg gctgagggga
    12961 tggaacgtta ccaaaatcca gatgtcccta tccgccccaa gggcgttgtc gaaaaatgca
    13021 ccttctgtac tcaccgcttg ccagttccag cttgtaacca ggcctgtccg ggaaacgcgc
    13081 gcttttttgg cgatttggaa gaccctgata gcgtggtatc ccaattagcg cgcagcccgc
    13141 gcgccttcag gttgttggag gatttgggta cagagccaaa agtttattac ttgcgagaag
    13201 gagcgtaatg tgaacaaaga cgataaagaa gctgtcttgt tcaaaccggt agaaaagacg
    13261 ggacgcgggt tttatctggt cgtcgctggt ctggtattga tccttgcctg ggctgcatat
    13321 gcctactcga gacagattgt ggttggtctg gatgtcacgg ggctgaacag gccggtttat
    13381 tggggcttgt acatcaccaa cttcgtcttc ttcatcggca tcagccatgc tgggacgttg
    13441 gtttcggcta tcctgcgcgt gtttggagct gaatggcgcc gcccaattac cagggctgcc
    13501 gaagccatca cggtgttcgc cttggtcctt ggaaccggca acatcatgat tgacatgggc
    13561 cgccccgacc gtttgttaaa catcattcgc tatgggcgct ttcaatcgcc tctggtgtgg
    13621 gatgtgacct ccttgacagt ttacctgact gccagcctca cctatctcta cctgcctctc
    13681 atccctgatc tggccatcct gcgcgatcgc cccgggttgt caccctggcg acgctggctt
    13741 tacaccaaac tctctatagg ctggcaggga accgaagaac agaaacatcg tctggaaagg
    13801 ctcatcggta ttatggcagt gttgatcata ccgattgctg tgtcggtgca caccgtggtt
    13861 tcgtgggtgt ttgccatgac ggttcagccc atgtggcaca gcactatttt tggaccgtat
    13921 tttgtgattg gcgccatctt ctccggcatt gccaccctaa tcatcgtgat ggtgatcctg
    13981 cggcgcgcat ttaatttgta tgcctatatc accgatcagc atttcagcaa cctgggattg
    14041 ttgctgatga tattcgcttt cctgtgggcc tactttacat tctcggagta cctgaccgag
    14101 ttctatggta acgaaccagc tggtatgcgg gtcttgttcg ccaagctggt tgaagaattc
    14161 tccttaatgt tctgggccat gtggctgttc aacttcatct tgccatttat cctgttgagt
    14221 aaccgccgca cgcgtaccgt gacaggcact gtatttacat cgatcctggt taatgtcggc
    14281 atgtggctgg agcgctacaa catcgtcgtt cctacgctca cgcgtccacg cctgccatcc
    14341 cccattactt catacacccc aacctgggtg gagtggtcgg tgctggttgg cagttttgcc
    14401 gtgtttgcca tgttgtatgt gctattttct aagctgttcc ctatcgtttc gatttgggag
    14461 gtgcgtgaag gggaagagat cgctgctgga ggggttgtgg cgggtcatca cgctgaggcg
    14521 ggtgcagact gatcatggaa aacgagctgc tcacccgcgc agaaggttac gcctatctgg
    14581 catcgatgtt ccggcgtcct gagaccgcca cagcggctga accgggttct accagtttgg
    14641 atatcgtcca ggcctaccgg cgcctcttcc tgggtccagg tcgtcccatc gtaagccctt
    14701 acgagtcagt ccacctggaa gggcagttaa tgggggccag cgctgctcgt gtggtcgagt
    14761 cgtatgccga agcaaacctg cgattgtctc ctgatgaaca cgagttgcca gatcatgtct
    14821 ccatcgaact ggcctttatg gcttaccttg cagaacagga gcaatgtgac cctgatcagg
    14881 cggaagtttg gcgggagcgc cagcgctgct ttcttctaga ccacttgggt cgctggttgc
    14941 ccctgttttg ccagaaggtc gaagccagcc ggatgcatcc tttctatagt gaggcagcgc
    15001 agatcacgcg gcagcttgtt ggatctgaca tggcagagtt cctgccttct gtacgaaaaa
    15061 ggcgttacgc caatatttgt cttgaagtgg atggcgcccg ctgcactctg tgctcgcttt
    15121 gcactgatag ctgcccgaaa gatgcgctgc acgttgtcag cacgccagca cagttaagcc
    15181 tcctttttga ctcatcccat tgcaatggtt gccgggtatg cctgcggctg tgtccagaac
    15241 aagcgatcaa cctaaagcgc aatcccccgc aggattttcc tgtgttggct gccaagcagg
    15301 ttgtcgcggt ggccgatcgc gctgtctgtc ctgagtgcca ccgcccgcac gttgctgagc
    15361 cgtggctgga tcgtttagcc gagcgtatgg gtggtggagc atttgctttg catacgctca
    15421 aattgtgccc tttttgtaaa gtggctgtag gaaacagcct gcctgtctcg agtggcatcc
    15481 cataggagtc gatttcaagt gttgagaaaa tatatcacgc tcatcatcat catcttcatc
    15541 ggattgttgc tctctccagc tgtttttgat gtcaggggac aagaatcaga gaccgaagat
    15601 accccgcaaa acgattatgt tggttcggtc acttgctctc gctgccatgc aaacacctat
    15661 gagacgttca aacagacctg gcatccgaat atgttgcgac ctgccacgga tgacaatata
    15721 ttaggtgatt tcaacaccca ggatcccgac ctggatttca cgcgcgaaga agtggattgg
    15781 gtgctgggtg ggcagtataa acaacgctac ctcacggaag aagatggtca gctatttgtc
    15841 cttctgtcag agtggaatgt cgagaccaga gagtggtctg cctatgtgac tggggagtgg
    15901 gtgccctatg agggccaaat ttggcgcaaa cgtccctatg cgccatattg tgcaggttgc
    15961 cacaccacgg gctttaatcc ggtgacccag acctgggtgg agcccggggt ggggtgcgag
    16021 gcatgtcacg gccccggggg ggatcacgtg gcctccactg gcaaccggac aaaaatcatc
    16081 aaccctgcca atctggaatt tcgagatcag gtcgaaatct gcgcccaatg ccacagccgt
    16141 ggtgaagacc cttcggagca attcccatat ccagtgggtt attacccagg tggtccagcc
    16201 aaattgggag agacctttat ccttaccacg gacccgaatg atttctggcc caatgggaca
    16261 gcgaaacgcc accacatgca atatcaggac tggcagatcg gtgagcataa agacagtgtg
    16321 agctgcatct tctgccacac ctctcacagc gttggcgaga ccgatcacca gacccgcatg
    16381 gtgggcaatg accgctgtgt gatttgccac gaaggtcaga cggacctggc tgcacatatt
    16441 ccttttatgg ctagcgtggc agatcaggtc aactgcaccg actgccatat gccccaaatt
    16501 tccaagctcg tccccaccga ttttgagatc ttgagtcaca cgtttagccc gcccgatcca
    16561 gcagtcagca ttgcctatgg tggttttgag cagatgccta atgcctgtaa tttgtgccat
    16621 accgacaaat cacccgaatg ggctgctggt gtgctcggac aagaaatccc aacagcggat
    16681 gcaaccccgg tgccccaacc aacaccaatg cctttgccaa caagcgtttt tgcccaggca
    16741 ggcctgtttg cgagtgaaga ggtcaacccc ccggatacac aagccggatc tggagctctc
    16801 acgctctggt ggttgggact cctggccgtg atcttagtat ttgctttctt atgggcacgt
    16861 cgcaggatga actctgaatg aaaataaact ctttgttcct aaccctcctc attttctttg
    16921 cttcgttact gcttgttggc tgtctgggag aagcagatct gccagccttg gaaagcaggc
    16981 cagtaccgcc cctcgcaccc ccggcggtgg atccaaccgt ggctgctttg ccccgtcaag
    17041 acatcttcac tggggcacag gtgtcggttt ctaaccagca agccccgttg ataccgcacg
    17101 acatgattgg tcggaccgac tgtctcatgt gtcacaaaca aggcgtgagt aatgccccgc
    17161 gcatcccaga tggccatcgg ggtttagaga gcaatacttg ccagacctgc cacgcctcgt
    17221 tagcaagcct cgagctgagc ggtgcggaga tgtaccagcg aatttgcact cgctgccatg
    17281 gtgaggacgg cgaggggggg gctgggccag cgctgaacac gaagtcctat ttgcgcaatg
    17341 tcactgacga agagatacgc gcagccatcg tacgcgggct gggcgaatcg cagatgctgg
    17401 cttggggtga tcttggattg ctaactgatc gccagatcga tgagctcgtt gaaatgatac
    17461 gagcctggga acctacagcc ccagaagtag caatcattgc ccaccagccc cccagcgcgg
    17521 cccaaggtga cccgggacgc ggctcggagc tatttgccca gttttgctcg ggttgtcacg
    17581 gtattgaggg ggagaccgta ttagtagatg ggtttgtgct cagccagttg gtcagccagt
    17641 tagaagatga cgtgatctcc aagcaaatta atgatggcac tgaagagatg ccatcattcc
    17701 atgccttgct taccagggat gatatcaacg atctactggc gttcatgcgc gagactttcg
    17761 aaggcggggg tgaaactagt atgacaagcc tgcctagttt tacacaggag atactccctc
    17821 tgttcaacca gaaatgcgtg ttttgtcacg gtgcgtctgg agggtggcaa tctgggagct
    17881 acgacgaagt gatgaatagc ggtatgaatg caccagtagt cattcctggc gatgcagagg
    17941 gcagccttct ggttcagaaa ctgcgcggca ctcaggcgga aggcgctccc atgccgcctg
    18001 gcacgcctct ttctgaaaca gagatcgatc tggtagtgcg ctgggttgaa gccggtgcgc
    18061 cggataactg agcggcttta gcctctggcg aagaacaaaa caaagatagg tttgggtgac
    18121 cccaggctac ctgtcgtagt atttttggaa gtgcgcttcc attgttgcct ctccggcctt
    18181 caggattggc tccatccgct cggctgagag atcaaattcg agaatgccat agcccttggc
    18241 tggcagctgg cacacaaagt gctggtgggc atcgatgatg gttttgtcgt gcgcgcctaa
    18301 taccgtctca gccataccct gaattcgcag catcatttct gccagcaggt ccatccgctc
    18361 caggaagctc ggggcatcgg attcgctttt ctgggtatct tctgcaccgg gtactggcat
    18421 actgtcatcc agcagcatac caattacctg ttccgaggca gaatccctgc ccatgatttc
    18481 ttcgatggta tcatcggtcg agaccagcag gttaatcggg aaattggaga gcagaccgcc
    18541 gtccaccacc ttgtgcccgc tcaggtctcg cccgcgatac atcccccatt cctttcgcca
    18601 gatcacctca tgccagacga aggggcagct catcgacatg cgcaccgccc atgttgttgg
    18661 gcaatctggc gctgtgcggt ggttcaacac caacatctca cgaccggtca catcggaggc
    18721 gaccaccgat aagtcccgtc ctgtggaatg gtgaaactcc agcagtgtgg actggcccag
    18781 attacgcccg cctgcatcca gcttctcatg caggtagccc atcagcgcgt tgccagagta
    18841 ccaacctccc caaaccagca aggagatcaa gtgtctggcg agatccttct gcaccatgcc
    18901 catcaccagc tggtccacta ccggttcgat catgttgggg atcattgggt tgtccagctc
    18961 tgtcttcagc cagtaacgca agctgttgtt gatctcgatc ttctcatcga tctggggcgt
    19021 ctccaagaag ctggtaaacc gcgaactgcc gtctggaagt ttttcgttga gggctgccat
    19081 gcactccgcg gcgttatatc ccgctgctat cagcacagcc aggattgacc ctgccgatgt
    19141 gccgatcacg cgccgcggcg tgtgccctcg ccgttcaaag gtttgtaaag caccgacaaa
    19201 tgccaggcct ttggcgccac cgccttcaaa taccaagtca aattccatct tagtctgttc
    19261 ctagatatca tgattttaag ggatgggacg gaatgtgatc ctgcatcccg cctaaggaga
    19321 tacattatac tggattttgc tattgatgga aaatattatt tttgttagaa tgctctaccg
    19381 aatccgccga ccgaggagat tctgatgctt gctgccttaa aatcgaaaat gatgcgcccc
    19441 ctcaccaata gactagctcg ccctgttgat gcggttgctc acgagcgact tcgtctgcgc
    19501 ctcctgtttg ccgtagcaat cctgaagctg tttaacttta ttggaggggc ctgggatatc
    19561 cagtggcatg tcgctatcgg ccgcgatagc ctgtttatcc cgccacactt gctggtcatc
    19621 ttcgcgtttg tcgcaggcct tgcgcttgtc ctggctctga
//
LOCUS       DBNGSX010000591         9038 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1379631, whole genome shotgun sequence.
ACCESSION   DBNGSX010000591 DBNGSX010000000
VERSION     DBNGSX010000591.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9038)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9038)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9038
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1379631"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="AC16CB_17210"
     CDS             complement(<1..268)
                     /locus_tag="AC16CB_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3344263.1"
                     /translation="MRKIAWIISIFVIAAVLLAACGGPQATEAPPPTEAPPPTEAPPE
                     GGIPDLGGQTIAVAVENAYPPFNTIDETTNEGVGWDYDTVREICA"
     gene            480..884
                     /locus_tag="AC16CB_17215"
     CDS             480..884
                     /locus_tag="AC16CB_17215"
                     /inference="COORDINATES: protein motif:HMM:NF014132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HIR3344264.1"
                     /translation="MPHFPSPEWLPSLKDKLNTDERYAQIAQNWEGDMLVFIEPDGAL
                     TQPFAYYLDLWHGKCREVYIVDQNQEVKPFLTLSGRYGIIVRVIKGELDPIQAMLTRK
                     LHVEGNMIVLVRNVPTVMEFIRCCRENTDSYT"
     gene            996..2174
                     /locus_tag="AC16CB_17220"
     CDS             996..2174
                     /locus_tag="AC16CB_17220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344265.1"
                     /translation="MKSRLTLVLFILFSAGIFFVTASIGVLGGYQTGMREYSRLQSLD
                     EVKFLQEQYLLAVQDFDSERYDLARQRFEYVLEQDPAFPGAAAKLVQVVQILYATATP
                     TAIPPTVTPTPTRDLRPVQDQFNQAQVDYSQGNWNSAIDVLLGLRKEDAAYNVVEVDS
                     LLYRSLRNRGLQKIREEANLEGGMYDLSLAENFGPLDTQADNLRNLARLYVIGSGFWE
                     VYPEQAVFYFSQVAAAAPGLRDASGWSASGRLWASLVQFGDFLAERDDWCAAEEKYNA
                     AFAMQSNTELMEKISQAYDKCHPPTETPKPTPKPSETATNTIVPPVITVETPTSTTLP
                     PAVTQTATRTATIQVTAPPPTTTEAPPPSPTNTSPPPTETDTPPPPPEEPTQTSTSES
                     "
     gene            2233..3621
                     /locus_tag="AC16CB_17225"
     CDS             2233..3621
                     /locus_tag="AC16CB_17225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR3344266.1"
                     /translation="MTTRVILVVFIFSLACLVGFASFVLAREFTVSNLTLTRKTGPEL
                     QESQATPVIEEQPQASEPSSFQPQIEPTLTPWDGASRVTVLLLGLDYRDWESGSGYAR
                     SDTMILLTLDPLTRTAGVLSIPRDMWTAIPGFKHGKINTAYYLGDAYKLPGGGPGLAV
                     KTVEQFLGVPINFYAQIDFGAFVRFIDEIGGVKINVPEPIKVDLLGDGYRTIKRLQPG
                     VQVLPGEIALAYARARYTEGGDFDRAERQQQVILGIRDRILSFDMLPLLIQNREALYQ
                     ELSTGVHTNLTLDQAVQLAIIASQVPQENIKRGVIGKGYVLFGQSPDNLSILIPLVDK
                     IHTLRDEIFASSSALSPLTPGSELERMLAEGARVSIENQSRTTGAGLAERTSQFLVNQ
                     GVNVASTSESPQNLTSTTLIDHTGKPFTARYLVNLIGISPGKIILDETPGSPVDIEIL
                     LGDDWARENPIP"
     gene            complement(3623..5911)
                     /locus_tag="AC16CB_17230"
     CDS             complement(3623..5911)
                     /locus_tag="AC16CB_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344267.1"
                     /translation="MSMIPYLVGLPPAKPEPLSRYLPPIPVGVVRTWLQTNLPRGAGE
                     TKAAWVLEPFGATPRVAIEAARAGYRVLVAANNPVTRFLLEMAANPPAEDELISALAE
                     LAASRRGDERLELHIRSLYHSNCNICSNEVEVEAFLWDRVVLSPQAEKPSPSGPVPFA
                     KIYHCPQCNTQGEHPITEEDLARAAQFSTSSLHRARALERVAPLDDPDRHHAEEALSV
                     YLPRSVYALFNLINRLDGMSLAPQRRNHLTALLLSACDLGNALWPYPSGRARPRALTI
                     PPKFRENNLWFVLEQAIQQWSHPSDQVALSTWPEQPPHEGGISIFEGRWRDLAAELAD
                     IPIEAVVTALPRPNHAFWTLSALWAGWLWGQEAVRPFKSVLRRRRYDWNWHTTALHAA
                     FENLPMILKPGSLFLGLIGEAEPGFLSAALVAADNANFELSGIAMPTEGGQAQINWRL
                     LSQVHDTPYSEEERVLRMRTAARDYLSTRGEPSHYLPIHTAVLSSLAQIHALRSSEQA
                     PMSMLVKINTAIDEALSYRGGLLRFGGSEKSMEVGYWWLRETDNISSALADRVEIELV
                     QYLITHPGASLPDIYSAICHTFPGLLTPSAELIQICLESYGEETSPGSGDWHIQAQNT
                     PSARKTDLKDIASLIIQMGANLGYQVEGDQPILWRDAANQVAYAWHVTASAVIGDAIF
                     KQDYAPGISLVALPGGRANLVAFKLQHDPRLRQAFEEGWRFVKYRHLRWLAQNPILSR
                     DTLDHLLTQDTLTYESPQMRLF"
     gene            complement(5962..6879)
                     /locus_tag="AC16CB_17235"
     CDS             complement(5962..6879)
                     /locus_tag="AC16CB_17235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3344268.1"
                     /translation="MHRLTAITGAFGYIGKYIARVLLENGERIVTLTNKPTPDDTLRE
                     KIPAYPLDFDQPEALVSALSSVDTLYNTYWVRFNYGQTTFDKAIQNTQTLIHAAKKAG
                     VGRIVHISIANPSTDSNLPYYRGKALLEQFIQQSGLTYAILRPTVVFGLEDVLINNIA
                     FLLRYLPIFAIPGSGNYRLQPIFVEDLAKIAFNAGHSSENQVLDAVGPDIFTYKFLVH
                     LIAEAIGSRSWIIHLPPSMALTLSRLIGLALNDVVLTRDELTGLMADLLVSREPPLGK
                     TSLRDWLASNAEACGTKYASEIRRHFSSP"
     gene            complement(6883..8331)
                     /gene="pyk"
                     /locus_tag="AC16CB_17240"
     CDS             complement(6883..8331)
                     /gene="pyk"
                     /locus_tag="AC16CB_17240"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR3344269.1"
                     /translation="MDRRTKIVATIGPASEDEATLTRMIHAGMNVARLNFSHGDHADH
                     AARIQRIRQISQRLEKPVTILQDLQGPKIRTGDLRNGSILLTPGDTLTLTTTPVLGEE
                     GLVSVDFSGLPGSVKQGGRILLDDGNLELIVTKVHGDRVETQVSLGGTLKPHKGVNLP
                     GAQLDVPSFTEKDEDDLSFGLEHRVDAVAISFVRSAQDILTIRQAIANIAPDRSWIPI
                     IAKLERPEAVVNLEEIVESADGVMVARGDLGVEMSPEAVPIAQKEIIESANNHARIVI
                     TATQMLDSMISKPRPTRAEASDVANAIFDGTDAVMLSGETAAGKYPVQSIETMNAIIS
                     QAEPHLSEWGHWKGTCMPELSQDDAYYMTLAARELAHDRNVAAIVVFTKSGRTALLMS
                     KARPHLPILAFTPDPNTYQRLGLYWGVYPNLIPQADTIEEMLQVVETAMVASSSMQAG
                     QQVVVICGFPVSQIRPTNLALLYTIGEISTYT"
     gene            complement(8343..>9038)
                     /locus_tag="AC16CB_17245"
     CDS             complement(8343..>9038)
                     /locus_tag="AC16CB_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HIR3344270.1"
                     /translation="AGHIAELIPDGATIQLGIGAIPDAVLKFLFEKKDLGIHTELFSD
                     GIINLVEAGVLTNARKTLHPGKIIAGLILGTRSLYDWVDDNPLIELHPSEYVNDPFVI
                     AQNERMVAINSAIEVDLTGQVCADSIGTQLFSGAGGQLDFIYGASRSKGGVPIIALPS
                     QAKNFSRIVTTLKPGAGVVTTRNHVRYVVTEYGVADLYGKTIRQRARQLINIAHPQFR
                     DQLTNEAKLLHYI"
BASE COUNT         2231 a         2167 c         2378 g         2262 t
ORIGIN      
        1 gagcgcagat ctcgcgcacg gtgtcatagt cccagcctac gccttcgttg gtggtctcat
       61 caatggtatt gaagggcggg taggcgttct ccaccgctac tgcgatagtc tggcctccca
      121 ggtcagggat tccaccttct ggcggcgctt cagtcggtgg cggtgcctcg gtaggcggcg
      181 gtgcttcagt agcttgaggc cctccacagg ctgccaatag tacggctgcg atcacaaata
      241 tggaaattat ccaggcgatt tttctcattg ctgtctcctt attatattat tttcagtgat
      301 gttaaacata ttgtgttttg tcatgtgcct cctctgttag tagcttggaa tttggttaga
      361 atacaagaaa attgcgaata gagcaattga aaacagtata atcataatac gtattcactt
      421 agttgaaaag ttgctcgttt attatttata aagttgttat atagagagag taaaaccaaa
      481 tgccccattt tccaagtcca gaatggctgc ccagcctgaa agataagttg aacacggatg
      541 agcgttatgc acaaatagcc caaaattggg aaggagatat gcttgtattt atcgaaccag
      601 atggcgctct tacccaacca tttgcttatt atttagattt atggcacggc aagtgtcgag
      661 aagtttatat cgtggaccaa aatcaggagg tcaagccgtt cttgacgctc agcgggcgat
      721 atgggattat tgttcgcgtc atcaagggcg aactcgaccc gattcaagcg atgctcactc
      781 gtaagcttca cgttgagggg aatatgattg tcctcgtgcg caatgtgccc acagtgatgg
      841 agtttatacg ttgctgccgg gaaaatacgg atagctatac ctaattggga acgtcgatgc
      901 cgcaatttgc gtctataagg ggcgagctct caactcgtta ttgatctatg caatctcaaa
      961 ctccaaatcg ctctccttcg caacgtcggg ctggcatgaa atccaggttg actttggtgc
     1021 tatttattct attctcggct ggaattttct tcgtgacagc atccattggg gtgttggggg
     1081 gataccaaac cggcatgcga gagtatagca ggttgcaatc ccttgatgag gttaaatttc
     1141 tccaagagca gtatctcttg gcggtacagg attttgattc ggagcgttat gacctggctc
     1201 gccaacgctt tgaatatgtc ttggaacaag atcccgcttt ccctggagcg gcggcgaaac
     1261 tggttcaggt ggtgcagatc ttgtacgcaa ctgccacccc taccgctatc ccgccgacgg
     1321 tcaccccaac gccgacgcgt gatttgaggc ctgtgcaaga tcagtttaac caagctcaag
     1381 tcgattattc ccaaggaaat tggaacagcg cgatcgatgt tttattggga ttgagaaaag
     1441 aagatgcagc ttataacgtg gttgaggtcg atagcctgct atatcgttca ctaagaaatc
     1501 gtggcttgca aaaaattcgt gaagaggcca atttagaagg aggcatgtac gatctttcgt
     1561 tagctgagaa cttcggtcca ctggacaccc aggctgataa tttgcgtaat ctggcgcgct
     1621 tgtatgtgat tggatctggc ttctgggaag tatatcccga gcaggctgta ttttatttca
     1681 gccaggtggc tgcagcagcc cctggactcc gcgacgcttc ggggtggtcc gcctcgggtc
     1741 gcctttgggc tagcctggtt caatttggag atttcctcgc ggagcgggat gattggtgtg
     1801 cggcagagga gaaatataat gcagcctttg ccatgcaaag caacacggag ctcatggaga
     1861 aaatatccca ggcctatgac aaatgccacc ctccgacaga gacgccaaaa ccgacgccga
     1921 agccctcgga gactgccacg aacacaattg tgcctcctgt gatcacggtt gaaacgccaa
     1981 catctaccac ccttcctcct gcggtgacgc aaacagcaac acgtacagcg acgatacagg
     2041 tgactgcccc gccgcctacg actacggaag cacctcctcc aagcccgacg aatacatcgc
     2101 cacctcctac tgaaacggat acaccaccgc cacctcccga ggaaccaact caaacctcaa
     2161 cttcggagag ctagaggcgt attagcaatg agacagataa ggaattttgt gtcaattcta
     2221 atcaagcatt caatgacaac gcgagtcatc ctcgtggttt tcatatttag cctcgcttgc
     2281 ctggtggggt tcgcgtcatt tgtgctcgcc cgggaattca ctgtctcgaa tcttacgctt
     2341 actcgcaaga ctggacctga gcttcaagag agccaagcca cgcctgtaat cgaagagcag
     2401 ccacaagcaa gcgaaccaag ctctttccag ccgcagatcg aacccaccct cacgccttgg
     2461 gatggcgcca gccgggtaac agtactgcta ttgggcctgg attaccggga ttgggagtcg
     2521 ggctctggct acgcgcgcag cgatacgatg attttgctca ccttggatcc gctaacacgt
     2581 acagctgggg ttctctctat cccgcgtgat atgtggacgg caatccctgg ttttaagcac
     2641 ggtaagatca acacggcata ctaccttggc gacgcgtaca aactgccggg tggaggacca
     2701 ggtctggcag ttaagacggt ggaacaattt ttgggcgtgc cgattaactt ttatgcccag
     2761 atcgattttg gtgcatttgt acgctttatt gatgaaattg gtggagtgaa aattaacgtg
     2821 cccgagccga tcaaggttga tctgctaggt gatggatacc gcaccatcaa aaggcttcaa
     2881 ccaggggtgc aggtgttgcc cggtgaaatc gcactggcat atgcccgcgc caggtacact
     2941 gaggggggtg atttcgaccg ggctgagcgc caacagcagg tgatcttggg tatccgggat
     3001 agaatcttga gctttgatat gcttccattg ctcatccaaa acagggaagc gctctatcag
     3061 gagttgtcta cgggggtaca caccaattta acactagacc aagctgtcca attagctatc
     3121 attgccagcc aggtgcctca ggagaacatt aaacgtggag tgattggtaa aggttacgta
     3181 cttttcggtc agtcaccgga taacctgagc atcttgatac cacttgttga taagattcat
     3241 accctcaggg atgagatttt tgcatcatcg agtgcgctta gtcccttaac accaggttca
     3301 gagcttgaac gtatgctcgc agaaggtgcg cgcgtctcaa tagaaaacca aagccgtacg
     3361 acgggagcag gcttggctga acgaaccagt caattcctgg taaaccaagg ggtgaatgta
     3421 gccagcacga gcgaatcgcc ccagaatttg acttcaacca ctttgatcga tcatactggt
     3481 aagccattca ccgccaggta tctagtgaat ttaatcggta tttccccagg aaagattatt
     3541 ttggatgaaa caccaggaag cccagtggat attgagatcc tgcttggaga cgattgggct
     3601 cgggaaaacc caataccgta gtttaaaaca accgcatttg tggggactcg taggtcaatg
     3661 tatcctgggt gagcaagtga tccagggtgt cacgcgacaa gatcgggttc tgtgccagcc
     3721 agcgcaggtg ccgatatttg acaaaacgcc acccttcctc gaacgcctgg cgcaagcgag
     3781 gatcgtgttg aagcttaaag gcgaccagat tcgcgcgccc gccaggtaag gcaactaaag
     3841 aaattccggg agcgtagtcc tgtttgaaaa ttgcatcacc aatcactgct gaagcggtga
     3901 catgccatgc gtaagcgact tgattcgccg catcccgcca aagaatgggt tgatcacctt
     3961 ctacctggta tccaagatta gcgcccattt ggatgatcag gctagcgata tcttttagat
     4021 ctgtcttgcg tgctgaaggg gtattctgag cctggatatg ccaatctcca cttccgggtg
     4081 aggtctcttc accataggat tccaggcaaa tctggatcaa ctcagcactt ggggtgagca
     4141 gtccagggaa ggtatggcat attgcggaat agatatcggg aagcgaagct cccgggtgag
     4201 taatcagata ttgcacaagc tctatctcca cacgatcggc aagcgccgaa gaaatattat
     4261 ctgtttcgcg tagccaccaa taaccaactt ccatggattt ttcactgccc ccaaagcgta
     4321 gaagacctcc acggtaagag agcgcctcat ctatggcagt atttattttc accaacatac
     4381 tcatcggagc ttgttcagac gaacgtaggg catgaatctg tgccaaagaa gacaggacgg
     4441 cagtgtgtat tgggaggtag tgacttggtt cacctcgtgt ggataaataa tctcttgcag
     4501 cagtgcgcat ccgcaaaacg cgttcttcct ctgagtacgg agtgtcgtgg acttggctca
     4561 agagtcgcca atttatttgt gcttgaccac cttcggtggg catagcaata cctgatagct
     4621 caaaatttgc gttatctgcc gcaaccaatg ccgcagatag gaagccaggc tcagcctcgc
     4681 cgatcaatcc taagaatagt gatcccggct tcaaaatcat tgggagattt tcgaaagcgg
     4741 cgtgcagggc ggttgtatgc cagttccaat catagcggcg cctgcgcaaa acgctcttga
     4801 atggcctgac tgcctcttgg ccccatagcc aacctgccca tagggcggag agtgtccaaa
     4861 aggcgtggtt gggtctgggt agggcagtta ccacggcctc aatagggata tctgccagct
     4921 ccgccgccag atctcgccag cgcccttcaa agatggagat acctccttca tgtggtggtt
     4981 gttctggcca ggtggataag gcaacttgat cacttgggtg agaccattgt tggatggctt
     5041 gctcaagaac aaaccataag ttgttctcgc gaaatttcgg cggaatggtc aaagcgcgag
     5101 gacgggcgcg cccagatgga tagggccaaa gggcgtttcc caggtcgcat gcgctgagca
     5161 agagagcggt tagatgattg cggcgctgag gggcaaggct cattccgtcg agcctattga
     5221 taaggttaaa cagagcgtac acagagcgag gaaggtagac tgacagggct tcctctgcgt
     5281 ggtggcgatc tggatcatcg agcggtgcaa cacgctccag tgcacgggca cgatgtaaac
     5341 tgctggtgga aaactgagca gcccgcgcta ggtcttcttc cgtgatggga tgctcacctt
     5401 gggtattgca ttgagggcag tgatagattt tggcaaatgg tactggccct gaaggtgagg
     5461 gtttctctgc ctggggagat aagacaacgc gatcccataa aaatgcctct acctcgactt
     5521 catttgagca tatattgcaa ttggaatggt ataaggagcg aatatgaagc tccagacgct
     5581 catcacctct gcgtgaggcg gccagctcgg ccagagcgga gatcagctca tcctcagcgg
     5641 ggggattggc tgccatttcc agaaggaagc gagtgactgg gttattggca gcgaccaata
     5701 cacgatatcc agcacgcgca gcctcgatgg caacccttgg agtagcccca aacggctcca
     5761 gcacccaggc agccttggtt tcgccagcgc ctcgcggcaa atttgtttgc aaccaagttc
     5821 taaccacccc tacgggaatt ggaggcaagt aacgggacaa aggttctggc ttggcaggag
     5881 gaagcccaac cagataaggg atcatagaca tagcagtttc tatggatagt atatagattt
     5941 cgtaaaaaaa tacaactggc tttacggtga tgagaaatga cgacggattt ccgatgcgta
     6001 tttagtgcca catgcttccg cattggatgc cagccaatcg cgcaaagatg tcttgcctaa
     6061 aggtggctcg cgggaaacca ataaatcagc catcagccct gttagttcgt ctcgtgtcag
     6121 gacaacatcg tttaaggcga ggccaatcag tcgagatagt gtaagcgcca tactgggtgg
     6181 tagatggatg atccacgaac ggctgccaat ggcttccgcg atcaggtgca ccaagaattt
     6241 ataggtgaat atatcaggtc cgacagcatc caatacctga ttttcgctgc tatggccagc
     6301 gttaaaagca atttttgcca gatcttcaac gaagataggc tgtaggcggt aattgcccga
     6361 gccaggaata gcgaaaattg gtaagtagcg caataggaaa gcaatgttgt tgatgaggac
     6421 atcttcaaga ccaaaaacaa ctgttggtcg aaggatagca tatgttagac cagattgctg
     6481 gataaattgt tccagaagtg ctttaccgcg gtaatagggg agatttgaat cagtcgaagg
     6541 gttggcgatg ctaatgtgca caatgcgccc cacaccagct tttttggcag cgtggatcag
     6601 ggtctgcgtg ttttgaatag ctttgtcaaa agtcgtttga ccataattaa aacgcaccca
     6661 ataggtgttg tacaatgtgt ccacactgga gagtgctgaa actagcgcct caggctggtc
     6721 gaaatctaag gggtaagctg ggattttttc tctaagggta tcgtcagggg tgggtttatt
     6781 ggtcaatgtg acaatgcgct cgccattttc aagtagaacg cgagcgatgt atttgccaat
     6841 ataaccgaaa gctccagtga tcgcagttag cctatgcatg gatcaagtat atgttgatat
     6901 ttctcctatt gtatagagaa gagcaagatt tgtcggacgg atttggctaa ctggaaaacc
     6961 acaaatgaca accacctgtt gtccagcctg catggatgaa gaagctacca tggcggtttc
     7021 aaccacttgg agcatttctt cgattgtatc ggcttgcggg attaagtttg gatacactcc
     7081 ccagtacaat cccagccgct ggtaggtgtt cgggtcgggt gtgaatgcca gaataggtaa
     7141 atgtggacgc gccttggaca ttaataaggc agttcgcccg ctcttggtga ataccacgat
     7201 ggcagctacg ttgcggtcat gtgcaagctc gcgtgccgcc aatgtcatgt aataggcatc
     7261 atcctggctt aactcgggca tacaagtgcc cttccaatgc ccccattcag acaggtgagg
     7321 ttctgcctgg ctgataatag cgttcattgt ttcaatcgat tgcacaggat attttcctgc
     7381 ggctgtctct cccgagagca ttacagcatc agttccatca aatatggcgt ttgctacatc
     7441 ggatgcctcg gcgcgtgtgg ggcgtggttt gctaatcatc gaatcgagca tctgtgtggc
     7501 ggtgatcacg atccgtgcat ggttattagc gctttcaatg atctcttttt gagcaatggg
     7561 cacagcctct ggggacattt caactcccag atcacctcgg gcaaccatga ccccatctgc
     7621 gctttcaacg atctcttcca gatttacgac agcttctggt cgttctaatt tggcgatgat
     7681 tggtatccag ctgcggtctg gagcaatatt agcgatggcc tggcggatag ttaggatatc
     7741 ttgggctgag cggacaaagg atatcgccac ggcatctact ctgtgttcta gaccgaagga
     7801 gagatcatct tcatcctttt ctgtgaagct cgggacatcc aactgcgcgc caggcaggtt
     7861 gaccccttta tgaggcttga gcgtaccgcc caaggatacc tgtgtctcga cgcggtcacc
     7921 gtgcaccttt gtgacgatta attccaaatt cccgtcatcc aaaaggatcc gtccgccttg
     7981 tttgacgctc ccgggcaagc ctgaaaaatc aactgagact aaaccctcct cgccgaggac
     8041 gggtgtcgtg gttagcgtaa gagtgtcacc tggagttagg aggatactcc catttcttaa
     8101 gtcccctgtt cgaatttttg ggccttgcag atcttgcaag atagtgacag gtttttctaa
     8161 cctctgagat atttgcctta tccgttgaat ccgggctgcg tggtcagcgt gatccccgtg
     8221 agagaagtta aggcgtgcaa cgttcatccc tgcatgaatc atcctggtga gggttgcttc
     8281 gtcctcgctg gcaggcccaa ttgtggcgac aatttttgtc cggcgatcca ttatttcctt
     8341 cgctatatgt aatgcaataa ttttgcttcg ttggtcaatt ggtctcggaa ttgtgggtgg
     8401 gcaatattga ttaattgtcg agcgcgctgc cgtatcgtct tcccatacag atcagccact
     8461 ccatactcgg tgacaacata acgtacatga ttgcgagtgg tcacaactcc agccccgggc
     8521 tttagggtag ttacgatgcg actgaaattc tttgcttggc taggcaaggc gatgatgggc
     8581 actcctccct tggagcggga tgcaccgtaa atgaaatcta attgcccgcc agcaccactg
     8641 aatagttggg taccgatact gtctgcacat acttgcccgg taagatcgac ttcgatggct
     8701 gagttaatag caaccatgcg ctcgttttga gcgataacaa aagggtcgtt gacatattcg
     8761 cttggatgga gctcgattaa cgggttatcg tctacccaat catatagaga gcgtgttccc
     8821 aaaatgaggc cggcgataat tttgcctggg tggagagttt ttcgtgcatt tgtaagcact
     8881 cctgcctcta ccagattaat gatgccatct gagaataact ctgtgtggat gccaagatcc
     8941 tttttctcaa aaagaaactt caatacggcg tcgggaatgg cgccaattcc caattgtatg
     9001 gtggcaccgt ctgggataag ctcggcaata tgcccagc
//
LOCUS       DBNGSX010000592         4373 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1173579, whole genome shotgun sequence.
ACCESSION   DBNGSX010000592 DBNGSX010000000
VERSION     DBNGSX010000592.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1173579"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="AC16CB_17250"
     CDS             <1..666
                     /locus_tag="AC16CB_17250"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3344271.1"
                     /translation="PQRDAILLSRLHEAGINLDGDTFLEEFNANLSAYYIERDTEFIE
                     HTTAYLLRSLLAEWGYTQVSEEVIQHILSGMYAVSQAHWKAEPDAHPTLRSLRELGYR
                     LGLISNASDDADVQALVDQVGFRTYFDVIITSAAQGIRKPNPRIFKTALDHWGVQPSR
                     AAMVGDSLGADILGAQNGGLYSIWITRRADTAANRAHEDTIQPDAVISALGELPQLLN
                     SLP"
     gene            complement(737..2122)
                     /locus_tag="AC16CB_17255"
     CDS             complement(737..2122)
                     /locus_tag="AC16CB_17255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3344272.1"
                     /translation="MSSATADVLICGAGICGVSAAYHLTVKQGFKRVILVDERAPMSF
                     TSDKSTECYRNWWPGPGDTMVRFMNRSIDLLEELAQESGNYFGLNRRGYVFLTADPQK
                     AAQYERTAREISQLGAGPLRIHRGLPADPSYMPSPDDGFQNLPTGADLILDPELIRQT
                     FPFVSERAVAMLHIRRCGWMSAQQLGAYMLDQAKARNLEVLQGKVTQVKVEGGRVRSV
                     TVETSLGSQSVATDHLVIAAGPYVKQVSSLVGVNLPVYNELHAKVAIQDYLGIVPPHV
                     PLMLWDDPIQLRWSAEEQRELAESHETRWLLETFPSGVHFRPEGKGQSAFLLGLWTYD
                     IQEQEPLPNPSYDPYYPEVILRGLTHMIPGLGVYIGRMRKPRVDGGYYCKTRENRPLV
                     GPLPVEGAYIFGAVSGFGIMAGMAGGELLAAHVAGLTLPDYANDFLLSRYENPQYQRL
                     LAQWETTSGQL"
     gene            complement(2268..3446)
                     /locus_tag="AC16CB_17260"
     CDS             complement(2268..3446)
                     /locus_tag="AC16CB_17260"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR3344273.1"
                     /translation="MKQQGYRRSIYVLLVGLLLPVLLFSCRFFTPENVQTIPDQPPTV
                     VVSTPVSPERTTTIEITGEDEVLVDLYTRVNPSVVNVTVYQRQSGDLLPVGQGSGFVF
                     DTNGNIVTNAHVVHAAEQVEVTFSSGVVRRAELVGEDLNSDLAVVRAENLPEDAVPLP
                     LGDMDSLAVGQSVVAIGNPFGLDGTLTRGIISALGRTIPALTPFSIPQSIQTDAAINP
                     GNSGGPLLNLAGEVIGVNAQIETGGTTRSNLGIGFAIPVSIVRLVVPDLIQDGVHHWA
                     WLGIRGGSLTPPLVEAMDITVDKGAYIAEVISRGPSSKAGLRGSNNTVRVNGRDIDVG
                     GDVITAIDGQPVNTFDDLLIYIALKSPDQEVNLTILRDGETLEIPVTLEERPESLEQP
                     "
     gene            complement(3577..4215)
                     /locus_tag="AC16CB_17265"
     CDS             complement(3577..4215)
                     /locus_tag="AC16CB_17265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344274.1"
                     /translation="MKLPIIKRAALFLVAQVAALMLMACYLVQALQPDGVTPSAPVPL
                     AQVVDLPTAVLPSPTNVPTETLPEPPTPTASSPPTLADPTATPIVPTTTITASAIPMT
                     PTVEPTPTDHILWSDASEYLGEERTVCGPVAGTHYAKESRGKPTFLNIGEDYPSPDRF
                     VVLIWVEYRDNFSNKPEDYYDGKTICAQGEILEYEGIFEIEVQSPSDIEVQQ"
     gene            complement(4227..4364)
                     /locus_tag="AC16CB_17270"
     CDS             complement(4227..4364)
                     /locus_tag="AC16CB_17270"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344275.1"
                     /translation="MDAALQQLDQHGVELPWGVEADAHSRWAMFYDPAGNLIEIVQFG
                     R"
BASE COUNT         1048 a         1216 c         1152 g          957 t
ORIGIN      
        1 ccgcagcgcg acgcgatact gctcagccgg ctgcacgagg cgggcatcaa cctggatggc
       61 gacacgttct tggaagaatt caacgcgaat ttgagtgcgt actacatcga gcgcgatacc
      121 gagttcatcg agcataccac ggcttacctg cttcgctcct tgctggccga gtgggggtac
      181 acacaagtct ccgaggaagt catccaacat atcctatcgg gaatgtacgc cgtgagccag
      241 gctcactgga aagccgaacc tgatgcccac ccaaccctga gatccctgcg agaactgggg
      301 taccgtctcg gcttgatctc gaacgcaagc gacgacgccg atgtgcaagc cctggtggac
      361 caagtggggt ttcgaaccta ttttgatgtg atcattactt cggcagccca gggaatccgc
      421 aagccaaatc cgcgcatttt caaaactgcc ctggatcatt ggggcgtgca gccctcgcga
      481 gccgcgatgg tgggcgattc gctgggggcg gatatcttgg gtgcccaaaa cggcgggttg
      541 tacagcatct ggatcacgcg ccgggcagat actgccgcca atcgtgccca cgaagacaca
      601 atccagccgg atgcagtcat ctcggcactc ggagagctgc cccagttgtt gaattcattg
      661 ccctaaagtt tttcgctaac cccttggatt acgtaaatcc atcccccaaa aaactgcaag
      721 aaacaaccat gctccctcag agctgaccgg aggtggtctc ccactgcgcc agaagccgtt
      781 gatattgggg gttttcgtag cggctgagca ggaagtcatt ggcataatct ggcagggtca
      841 aaccagccac atgggcagcc agcaactcac cgccagccat gccagccatg atgccgaagc
      901 cggataccgc accaaaaata taggcaccct ctaccggtaa agggcccacc aaggggcggt
      961 tttcccgcgt cttgcaataa taaccaccat ccacccttgg ttttctcatc cgcccgatat
     1021 aaacacccag tcccgggatc atatgagtca ggccgcgcag gattacctcc gggtagtatg
     1081 ggtcgtagct agggttaggc agcggttctt gttcctggat gtcatatgtc cacaacccca
     1141 acaaaaacgc gctctggcct tttccctccg gacggaaatg cacaccggac gggaaagttt
     1201 ccagcaacca gcgagtctca tgactttcag ccaactccct ttgttcctct gcactccaac
     1261 ggagctggat tggatcatcc catagcatca agggaacatg cggaggaaca attcccagat
     1321 agtcttggat ggctacctta gcatgcagct cgttataaac tggcagattc acgccaacca
     1381 atgaagatac ctgtttcaca taagggccag ctgcaatcac cagatggtct gtcgccacgc
     1441 tttgggaacc gagcgaggtt tcaacggtga ccgagcgcac gcgcccgcct tctaccttta
     1501 cttgcgtgac ctttccctga agaacttcaa gattcctggc ttttgcttga tccagcatgt
     1561 aagctcccaa ttgctgggcg ctcatccatc cgcagcgccg tatatgcaac atggctaccg
     1621 cccgctcgct cacaaaaggg aaggtttgac ggatcaactc tggatcaaga attaaatctg
     1681 ctccagtggg aaggttttgg aagccatcat caggcgaggg catgtagctt ggatcagcgg
     1741 gcaggccgcg atggattcgc aaagggccgg ctcctagctg agagatctcg cgtgcggtgc
     1801 gctcatattg agccgctttt tgcgggtctg cggtaaggaa gacatacccc ctgcggttca
     1861 aaccgaagta attaccgctc tcctgcgcca gttcttccag cagatcgata ctgcggttca
     1921 taaaccgcac catggtgtcg cccgggccag gccaccaatt gcggtagcac tcggtggact
     1981 tgtcgctggt gaagctcatg ggagcacgct catccaccag gatgaccctc ttaaagccct
     2041 gtttgacagt aagatgatat gctgccgaga caccacaaat cccggcacca cagatcaaca
     2101 catcggctgt agcagaagac atgaggctat tcagataccc ttggcgatta tctgtcgtca
     2161 cccccctcaa gtgggaggtg acgacaatgg aagtatcagg aattacgagc taaacaagaa
     2221 gggtggttgc aaaacaccct gcccaacacc ttttgaggtg aggactacta tggttgttcc
     2281 aggctctcag ggcgttcctc taaagtaacc ggaatctcca gggtctctcc atcgcgcagg
     2341 atggtcaaat tgacttcttg atctgggctc ttcagggcaa tatagatcaa cagatcgtca
     2401 aaagtattca ccggttggcc atcgatagcg gtaatcacat cgccgccaac atcgatatca
     2461 cgcccattaa ctctcacagt attgttcgaa ccacgcaagc cagccttgga cgatggtcct
     2521 ctagagatga cctccgcaat gtaagcgcct ttatctactg ttatatccat agcctctacc
     2581 aatggaggag tcaggctgcc gccgcggata cccagccatg cccaatgatg cacgccatct
     2641 tggatcaagt cgggaaccac caggcggacg atgctcactg ggatggcaaa cccaataccc
     2701 aggttgctgc gtgtggtgcc accggtttcg atctgagcgt tgacaccaat aacctcacca
     2761 gccaagttga gcagcgggcc gccagagtta cccgggttga tagcggcgtc cgtctggata
     2821 gactgcggga tagagaaggg ggtgagggct gggatggtgc gccccaaggc actgatgatg
     2881 ccacgcgtta gagtgccatc caatccaaag ggattgccga tggcgactac cgattgccca
     2941 accgccagcg aatccatatc acccaggggc aatggaactg catcctcggg caggttctct
     3001 gcgcgaacca ccgccaggtc gctattcaaa tcttcaccga caagctcggc gcgacgcacc
     3061 acgccactgg agaaagtcac ctcaacctgt tcggcagcat gcacgacatg agcattggtt
     3121 actatgtttc cgttggtgtc gaatacaaag cccgatccct gcccaaccgg gagcaaatcg
     3181 ccgctttgtc tctggtacac ggtcacattc accactgatg ggttcacccg cgtgtagagg
     3241 tcaaccagaa cttcatcttc accggtaatt tctatggtag tagttctctc cggggaaacc
     3301 ggtgtgctga cgacaaccgt cggcggctgg tctgggatag tctgcacgtt ctctggggta
     3361 aagaatcgac aggagaacag tagcacaggc aacaataaac cgaccaggag aacatagatt
     3421 gatctacgat atccttgttg tttcatagtt aatctttcta acctccgata ggttagtata
     3481 gtcctctaag ttaaatattt ggttagaaaa tcatattaaa aagatcaaaa gttgcgcagc
     3541 caggcaagtc ttgttcgact gcgcaagtcc taaagtttat tgctgtacct caatatctga
     3601 tggagactgg acctcgatct caaaaatgcc ttcgtactcc aggatttccc cttgggcaca
     3661 gatggtcttc ccatcataat aatcttctgg tttgttggag aagttatccc gatattccac
     3721 ccagatcagc acaacgaaac ggtcgggcga tggataatct tcgccaatgt tcagaaaagt
     3781 aggtttgcct cgcgattctt tcgcgtagtg tgtgcctgcc accggaccgc acacagtacg
     3841 ctcctcaccc aggtattcgc tggcatcaga ccaaaggata tgatccgttg gggtcggctc
     3901 aacggtgggc gtcatgggaa tagccgatgc cgttatcgtt gttgtgggca cgatgggtgt
     3961 tgctgtggga tcggcaagag ttggagggct agaggccgtc ggggtcggtg gttcaggcag
     4021 ggtttccgtt gggacattcg taggtgaggg cagtacagcg gtaggcaaat cgacaacctg
     4081 cgccagcgga acgggcgcgc tgggagtgac gccatctggt tgaagcgctt gaaccaaata
     4141 gcaagccatt aacataagcg cagcgacctg ggcgacgagg aataaggcgg cgcgtttaat
     4201 gataggtagt ttcatgtctg tcccccttag cgaccaaact gcacaatctc aatcaggttc
     4261 cccgctggat cataaaacat tgcccagcga gaatgcgcgt ccgcctctac gccccagggc
     4321 aactcgacac catgttggtc aagctgctgg agggctgcat ccagattgtc tac
//
LOCUS       DBNGSX010000593         1719 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1657836, whole genome shotgun sequence.
ACCESSION   DBNGSX010000593 DBNGSX010000000
VERSION     DBNGSX010000593.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1657836"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1356)
                     /locus_tag="AC16CB_17275"
     CDS             complement(70..1356)
                     /locus_tag="AC16CB_17275"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019588037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR3344276.1"
                     /translation="MQHTIPVETLGAMGEHMANAVGKCVHCGFCLPACPTYNLLGEEM
                     DSPRGRIILMKSVLEGEVELEEALPYIDRCLGCLGCVSACPSGVPYGELVTPFRSFAQ
                     ERRRRPLTQRAARQLALDSLPSPERFRIAAKAGKAARPFQGVLPKQVRAMLDLLPGVL
                     PVSQPLPDLFPALGPRKARVALLTGCAQQVLAPQINWSTLRVLARNGVEVVIPQGQGC
                     CGALALHAGEISLARAQATYNLRLFPKDVDAVVTNAAGCGSGMRDYPLLFSLSQNGVD
                     EEDGWSDLVEQFAHKIYDISAFLDDLGIEKPPPLPNPLKLAYHDACHLVHAQGIMEAP
                     RRLLRLIPNLTLVSLPESDLCCGSAGLYNIEQPETARRLGERKAMHIASVEAQAVVTG
                     NIGCIVQMRTHLAQLGHSLPVWHTIEVLDRAYHGWG"
     gene            complement(1385..>1719)
                     /locus_tag="AC16CB_17280"
     CDS             complement(1385..>1719)
                     /locus_tag="AC16CB_17280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344277.1"
                     /translation="EAELWRSAREFLWVKPGHTLVKVPLTSQRVPPLDEWLADHGALR
                     RYSAGGNLAWIAWQGSIEPLHALLQEQGFSGLAILGSVDQPFLGKRVGDAFARRVKNA
                     LDPQGKFG"
BASE COUNT          386 a          510 c          475 g          348 t
ORIGIN      
        1 tgaaccagtg gtttcaaggt cttcagggct tcgttagctt gttcaacagt gaagtagcgc
       61 ggcatctttt caaccccatc catgataggc tcgatcgagc acttcgatag tgtgccagac
      121 cggtaaagaa tgccccagct gcgctaaatg ggtgcgcatt tgtacaatgc agccgatgtt
      181 gcccgtgaca accgcttggg cttcgacgct cgcgatgtgc attgctttgc gctcgcctag
      241 tcgacgagca gtctcgggtt gctcgatatt atataagccc gccgacccac agcacagatc
      301 actctcgggg agagatacca gggtcaggtt ggggattagc cgcaatagcc ggcgaggtgc
      361 ttccatgatg ccctgggcat gtaccaggtg gcaagcgtca tgataagcca gtttgagcgg
      421 attcggcagt ggtggaggtt tttcgatacc caaatcatcc aggaaggcgc tgatatcgta
      481 gatcttatga gcaaattgct cgaccaaatc tgaccagcca tcttcttcat ccaccccatt
      541 ctggctcaag ctgaacagca agggataatc tctcatccct gacccacacc cggcggcgtt
      601 ggttaccact gcatcaacat ccttggggaa gaggcgtaga ttgtaggtgg cctgagcgcg
      661 cgccagggaa atctcgccag cgtgcaaagc cagcgccccg caacaacctt gcccttgagg
      721 gatcaccacc tcaacgccat tgcgcgccag aacacgcagg gtagaccaat tgatctgagg
      781 ggcaagcacc tgttgggcac agccggtcag cagcgccacg cgtgcttttc taggtccaag
      841 ggctggaaat aaatctggta ggggctggct gaccggcaac accccgggga gcaagtccag
      901 catagcgcgc acctgcttag gcagcacccc ttgaaacggg cgggcagcct tgccagcttt
      961 agctgctata cggaacctct caggggatgg caggctatcc agagctagtt ggcgggctgc
     1021 gcgctgagtc aaaggcctgc gccggcgctc ctgggcaaag gaacggaacg gcgtgacgag
     1081 ttcgccataa ggcactcccg aggggcaggc gctgacacag cccaggcatc ccaggcagcg
     1141 gtctatgtat ggcaaggcct cttccagctc aacctccccc tccaagacgg atttcatcag
     1201 aataatccgc ccgcgcggtg aatccatctc ctcaccaagc aagttatagg tggggcaggc
     1261 tggcaggcaa aaaccgcaat gcacacattt tcccactgcg ttagccatgt gctcgcccat
     1321 ggcacctaaa gtttcaaccg gtatagtgtg ttgcatactc agcacatgtc ctgatattca
     1381 tggttcaccc aaacttaccc tgggggtcta aggcgttttt caccctgcgg gcaaaagcat
     1441 ctccaaccct cttcccgaga aaaggctggt cgactgaacc taaaatcgcc aggccagaga
     1501 acccctgctc ttgcagtaat gcatgcaacg gctcgatcga gccctgccag gctatccagg
     1561 ccaggttgcc ccccgcgctg tagcggcgta atgcgccgtg atctgcaagc cactcatcca
     1621 agggcggaac gcgttgcgag gtgagtggca ctttcaccag ggtgtgtccc ggcttcaccc
     1681 ataaaaattc acgcgccgag cgccatagtt cagcctcgg
//
LOCUS       DBNGSX010000594         2349 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_65913, whole genome shotgun sequence.
ACCESSION   DBNGSX010000594 DBNGSX010000000
VERSION     DBNGSX010000594.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_65913"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="AC16CB_17285"
     CDS             <1..939
                     /locus_tag="AC16CB_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HIR3344278.1"
                     /translation="MRVIITGGTGLIGRALVNELNNTDYELIILSRNPQKTNGLPYNV
                     RVEQWDAQTSEGWGSLVEGAEAIVNLAGESIAGDSMKSLILDRWSPARKRTILESRVK
                     AGRAIIQAVQQAKQKPKVVIQASAVGYYGNRGDEELLESSSSADDFLAQVCKEWEAST
                     AEIESMGVRRAIIRTGGVALSTEGGAFPFMLLQSKLFVGGPIGSGKQWFSWIHIADEA
                     RAIRFLIENTEASGAFNLCAPQTITNADFSRTLGRVLKRPSFFPVPGFAMSLTIGEKA
                     GLLLGSQKQIPKHLLDLGFSFKFPDAESALRDLLIK"
     gene            936..1379
                     /locus_tag="AC16CB_17290"
     CDS             936..1379
                     /locus_tag="AC16CB_17290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3344279.1"
                     /translation="MRRYRHTFTVKANKQAVADFHSHSSSMASITPPPVIVQMHKAPE
                     NLGENSEMEFTLWLGFLPIRWLASIEDISPTGFTDRQIRGPFKEWSHRHTYIPIDEKH
                     TEIIDEISYSFKEDLYWGLIGRGMATSLPLLFAYRAWKTRRILKS"
     gene            1411..>2349
                     /locus_tag="AC16CB_17295"
     CDS             1411..>2349
                     /locus_tag="AC16CB_17295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3344280.1"
                     /translation="MNSATPRVVVIGAGIGGLTTAAVLAREGMDVTVLEAQVYPGGCA
                     ATYYHQGYRFDAGATLAGGFYPGGPMDLVARATGIKEWQAHLAQAAMMVHLPDDQKIT
                     LWNDERRWSERERLFGDQGIRFWRWQERTADAMWDLAMRLPPWPPQSYRELAGLTQLA
                     SSWLINLPNKTITSQLLSDAVRPVAAHLKNTPDVLRLFTDAQLLISAQATSDRVNALY
                     GASALDLSRRGVVHLEGGMGSIAQSLVRIIQQHGGQVMFRQEATRVALQKGTPKHVET
                     KRGSIFPADIVIANLPPANLRNLLGDNAPSRIKNAST"
BASE COUNT          606 a          555 c          612 g          576 t
ORIGIN      
        1 atgcgggtaa tcattacagg aggcactgga ttgatcggaa gagctctggt caatgagctc
       61 aacaacaccg attatgagct catcatactt agccgcaatc ctcaaaaaac aaatggcttg
      121 ccatacaatg ttcgcgttga acaatgggac gctcagactt cggagggatg gggatcgctt
      181 gttgaaggag ctgaagccat tgtcaacctg gctggcgaat cgattgcagg tgattcaatg
      241 aaatctttga tactagaccg ttggtcaccg gctcgcaagc gcacaatcct ggagagccgc
      301 gtaaaggctg gtagagcgat tattcaagct gttcagcaag ccaagcagaa gcccaaagtg
      361 gtgattcagg cttccgcggt tggctactat ggcaaccgag gtgatgagga actacttgaa
      421 agctcatcat ccgctgatga ttttttggct caggtttgca aggagtggga agccagcacg
      481 gcggaaatcg agagcatggg agtacgcaga gcaatcatcc gcaccggggg cgttgcactc
      541 agcaccgaag gtggagcttt tccctttatg ctcttgcagt ctaagttatt cgtaggcggt
      601 cctattggct caggaaagca gtggttttcc tggattcata ttgctgatga ggcgcgcgct
      661 atccgctttc tcattgagaa taccgaggca agcggggcat tcaatttatg cgcaccacag
      721 acaattacca atgcggattt ctcacgcact ttggggcgtg tcctaaaacg gccttcgttt
      781 ttccctgtac cgggatttgc gatgtcgctc acaatcggcg agaaagccgg cttgctactg
      841 ggtagtcaga agcaaattcc caagcactta ttggatctgg gtttttcctt taagtttccg
      901 gatgctgaat cagccttacg ggatttgttg atcaaatgag acgctatcgg cacacattca
      961 ctgtcaaagc taacaaacag gcagtcgctg attttcattc tcattcatcg agtatggcat
     1021 ccatcacacc tccgccagtt attgttcaga tgcacaaagc accggaaaat ttaggtgaaa
     1081 atagcgagat ggagttcacg ctttggttgg gcttcctgcc gattcgctgg ctggcaagca
     1141 ttgaagatat atcccccacc gggtttactg accggcaaat ccgtgggcct tttaaggaat
     1201 ggtcccatcg tcacacatat atcccaattg acgagaaaca cacggagatc atcgatgaaa
     1261 ttagttattc attcaaagag gatctctatt gggggttgat aggcagggga atggcaacga
     1321 gcttgcctct actgttcgct tatcgcgcct ggaagacacg gcgcatattg aaatcataat
     1381 caatttttcc aattgagatt gttggtgata atgaatagcg ctacgccacg ggttgtagta
     1441 attggtgcgg ggattggggg cttgaccaca gctgcggttc tcgctcgcga aggtatggac
     1501 gtgacagtct tggaggctca ggtttacccc ggaggctgcg cagcgacata ttatcatcaa
     1561 ggctatcgtt ttgatgctgg agcaacatta gcaggaggct tctatcctgg cggcccaatg
     1621 gaccttgtcg caagagcaac cgggatcaaa gaatggcagg ctcatcttgc gcaagccgcc
     1681 atgatggtgc atctccccga cgatcagaag atcacgcttt ggaatgatga gcgccgttgg
     1741 agcgagcgtg aaaggctatt cggagaccag ggaattagat tttggcgatg gcaagaacgc
     1801 actgcggatg cgatgtggga tctcgcaatg cgtctccccc cttggcctcc ccaatcttat
     1861 cgtgaactcg ccggcctgac tcagctcgcc tcatcctggc tgataaactt gccaaataaa
     1921 accatcacat cgcaactatt atctgacgca gtcagaccag ttgcagcaca tcttaaaaat
     1981 acgcctgatg tcctacgcct atttactgat gctcaattat tgatatctgc tcaggcaacg
     2041 agcgacagag tgaatgcact atatggtgca tctgcattgg atttgtctag gcgtggagta
     2101 gttcaccttg aaggcggcat gggcagtatc gcccaatccc tggtgagaat catccaacag
     2161 cacggcgggc aggtaatgtt tcgccaagaa gccacacggg ttgcattaca aaaagggact
     2221 cccaaacatg tggagacaaa aaggggtagt atattccctg ccgacattgt catagcaaat
     2281 ttaccccctg caaatttacg aaatttatta ggagataatg ctccgagcag gataaaaaac
     2341 gcgtcaaca
//
LOCUS       DBNGSX010000595         2466 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_342083, whole genome shotgun sequence.
ACCESSION   DBNGSX010000595 DBNGSX010000000
VERSION     DBNGSX010000595.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_342083"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2145
                     /locus_tag="AC16CB_17300"
     CDS             <1..2145
                     /locus_tag="AC16CB_17300"
                     /inference="COORDINATES: protein motif:HMM:NF019313.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HIR3344281.1"
                     /translation="HKNTRTPLAIGVSGPWGSGKTTLLRRVQRQLDQTASLLEKSEPA
                     AIEFVNPDETPEKDFRVCRTVWFNAWKYADEDALLVALVRVIVQTMYADDFISKGLAT
                     IFEPFTDRRDVIDTVLGWFSIKTPFGDVKPNTGEPQPTAFTENSAMLDLFNKAFDRLM
                     AAWVHRKLDVKKINPQDGVLVVFIDDLDRCLPAKAVQVLEAIKLFMDKPGCVFVLGAD
                     AEVVRQAVESHYQNTKVTGQNAADYLEKVIQLRFDLPPVPDADMQEFLKEQQVSDEML
                     VEWRTLLAAAEVNPRRVKAVLNDIELKWRMLVNSGQAAGVSRGDFIRWSALMRAAPAS
                     FRVRVADIDDLDLRFKFVQDALRWGGGEADETLARTFQEYEKDGRRMRRVLRQIKAFS
                     PEFDAHTLDAFIHLAAPPSSPTLAGEPAERAVSGEKIGGRLAGPEVARVGSITWGGIE
                     FVRVPSGRFLMGSKEDNELAYDDEKPQHTLDILQEFGIGRYPVTNAQFEAFVERTNYR
                     TIAEVEGSGYTWDGKEWKDVKGADWRHPRGPKSNLDGKENHPVVLVSWLDARAYCEWL
                     NQEHSEDLPGGWKFRLPTEAEWEKAARGEYGNEWPWGDEQPDAKWCNFNMNVGDTTPV
                     GQYSPQGDSPYGAADMVGNTWEWTHTLFKDYPYDPADGREDSAGSGTRVLRGGSFSDV
                     LKIVRGASRGGNSPDFRFIHSGFRVVASSIHL"
BASE COUNT          528 a          684 c          773 g          481 t
ORIGIN      
        1 cacaagaaca cgcgcacccc gctcgctatt ggtgtaagcg gaccatgggg ttcgggaaag
       61 accacgctgc tacgccgcgt ccagcgacag ctcgaccaaa ctgccagctt gttagaaaaa
      121 agtgagccgg ctgccattga gtttgtcaac ccggacgaga cccctgaaaa ggatttccgc
      181 gtttgccgca cagtatggtt caacgcctgg aaatacgccg atgaagatgc ccttctggtg
      241 gcgctggtgc gtgtcatagt gcagacgatg tacgcagacg acttcatctc caagggtctg
      301 gctaccatct tcgagccatt cactgaccgg cgggacgtga tcgatacggt gttagggtgg
      361 ttctcgatta agaccccctt cggggacgtg aagcccaaca ctggggagcc tcagcccacc
      421 gcctttacag aaaattcagc tatgctggat ctatttaata aagcgtttga ccgcttgatg
      481 gccgcctggg tgcaccgcaa gctggatgtg aagaagatca atccccaaga cggtgtgcta
      541 gtggtgttca tcgatgatct cgatcgctgc ctgccggcca aggcagtcca ggtgctggaa
      601 gccatcaagc tgttcatgga taaacccggc tgcgttttcg tgctgggagc ggatgctgaa
      661 gtggtgcgcc aggctgtgga gagccattac cagaatacca aagtcaccgg gcagaacgcg
      721 gcagattacc tggaaaaggt aatccagctg cgcttcgacc tgcctcccgt accggatgct
      781 gacatgcagg aattcctcaa agagcagcag gtgagcgacg aaatgctggt tgaatggcgc
      841 acgctcctgg cggcggccga ggtcaacccg cgccgggtga aggcggtgtt gaatgatatc
      901 gagctaaagt ggcgcatgct ggtcaactcc gggcaggcag ccggtgtgtc gcggggagat
      961 tttatccgct ggagcgccct gatgcgcgcc gctcccgcca gcttccgggt ccgcgtagca
     1021 gatatcgacg acctggacct gcgctttaag ttcgtgcagg atgcactgcg ctggggaggt
     1081 ggggaggccg atgaaaccct ggcgcgcacc tttcaggagt atgagaagga tgggcgccgc
     1141 atgcgccgcg tgctgcgcca gatcaaggct ttcagcccgg aattcgacgc acataccctg
     1201 gatgccttta tccacctggc agctccacca tccagcccta ctctggccgg cgaaccagcc
     1261 gagagagccg tgtcaggcga aaaaattggc ggacgcctcg caggccccga ggtcgcgcgg
     1321 gttggctcga taacctgggg tggaatcgag ttcgtgcgcg tgccgtctgg caggttttta
     1381 atgggcagca aggaagataa cgagctggca tatgacgacg agaaacccca gcacacgctg
     1441 gatatccttc aggaattcgg gatcggccgc tacccggtga ccaacgccca atttgaggca
     1501 ttcgtggagc gcactaatta ccgcaccata gcggaggtgg agggatcggg atatacctgg
     1561 gacggtaaag aatggaagga tgtaaaaggc gctgactggc ggcacccgcg cggcccgaaa
     1621 agcaacctgg acggaaaaga aaaccacccg gtggtgctgg tatcttggtt ggacgcccgg
     1681 gcttactgcg agtggttgaa ccaggaacac agcgaagatc tgcccggtgg gtggaagttt
     1741 cgcctgccca ccgaggcgga gtgggagaaa gcggcccgcg gcgagtatgg caacgagtgg
     1801 ccctggggag acgagcaacc agacgccaag tggtgcaact ttaatatgaa cgttggcgac
     1861 acgaccccag tggggcagta ctccccacag ggcgatagcc catacggcgc tgccgacatg
     1921 gtgggcaaca cctgggaatg gacgcatacc ctgttcaagg attaccccta cgatccagcg
     1981 gatgggcgcg aggacagcgc tggctctggc acgcgcgttc tgcggggcgg ctcgttcagc
     2041 gatgtactca agatcgttcg cggtgcctcc cgcggcggga acagtccgga cttccgtttc
     2101 atccacagcg gttttcgggt ggttgcgtcc tccatccatc tctgaactct ggttctctga
     2161 tctctgatct ctgactcctt gggtggaaag tcgtgctcct tcaagaacaa ctctatacct
     2221 gggaaaacct gtggctggcg tatcaaaatg cagcgcgcgg caagcgtggc cggggtgcaa
     2281 gcccgcaggg gcgcctgccc tgagcttcat ttagggtggc aatctcctga catttctatg
     2341 agatcgcttc gcctgccaga ttgcggcagg ctcctaagaa actgtcactg cgagtttgtc
     2401 ccaacgaagc tcgttgggac gagcgaagca gtctcagccg tcgttgcgct gagattgctt
     2461 cagtcg
//
LOCUS       DBNGSX010000596         3712 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_550865, whole genome shotgun sequence.
ACCESSION   DBNGSX010000596 DBNGSX010000000
VERSION     DBNGSX010000596.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_550865"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /gene="rlmB"
                     /locus_tag="AC16CB_17305"
     CDS             <1..739
                     /gene="rlmB"
                     /locus_tag="AC16CB_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIR3344282.1"
                     /translation="MREWINGRNPVYEVLRAARRQVFRLQIAQGVQEKGRLAEIASLC
                     QRGRIPIERPQRNQLDARFPNNQGVALEVSGYPYSDLIDVLALAEKRKEPPLLLILDT
                     LQDPQNLGTLLRTAEIVGVHGVLLPLRHTATVTPAVVHASSGASEHLLITQLNLAQAI
                     ETLKREDVWVVGLEGDPAEAVPSDVRLDGPLALVVGSEGEGMRSLVRKSCDVLLRLPM
                     RGHVESLNAAVAGSVALYLAWQARRWG"
     gene            complement(832..2181)
                     /locus_tag="AC16CB_17310"
     CDS             complement(832..2181)
                     /locus_tag="AC16CB_17310"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3344283.1"
                     /translation="MSSEIQNKYVVAVIGAGPAGLFGARELASQGAHVLLFNRDIKPG
                     GLAEYGIYPSKHKMKEGLRKQFRQILQNPNIDYYGNVIIGGQGDLTLDDIKDAGVQAL
                     LVTVGAQGTKWLGLPGEELSKGVYHAKDIVYHYNKLPPFSQENFHIGNRVALVGAGNV
                     MLDIARWLIRDLKVDEVIAVVRRGPAEVKFDKKEMESVASNLDLKGLDEEIARVAPAM
                     EAVGQDIQAAKDFILSALPKAYESVSESRLLFDFLASPVRILGDASGQVQGLEVEETK
                     LIPFNGDTKARGTGIKRILDVDTVIFCIGDKVDETFGLPVQWNEFVKNPNPSYPVNGI
                     SHEVFDAQTGQPIQGIFVAGWSREASAGLVGVARKDGESGAQAMLQYLQTMPAMENPT
                     AVADNVNKLLQKISKPIITKANILRLEAVEQSEAERRNLDEFKFGSNEEMLAAIGSG"
     gene            2520..>3712
                     /locus_tag="AC16CB_17315"
     CDS             2520..>3712
                     /locus_tag="AC16CB_17315"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3344284.1"
                     /translation="MLKEIHTAPIISHWVWYRVGSRNEVAGFTGVSHWVEHMQFKGTP
                     QFPSTVLDKAISREGGFWNAFTYLDWTTYFETMPAEKIDLALRLEADRMVNSQFDPQE
                     VASERTVIISERQGNENEPLFRLGEELQALAFRVHSYHHEVIGDLADLETMQRDDLYR
                     HYRQYYTPNNALIAVAGDFESEVMLGRIRELFELIPAGEALLQRIRPEPAQSGERRFT
                     LEGPGETTYIQFAHRAPAASDPDFFPLTVLDSLLTGPSSLNMFGGGISNKTSRLYRAL
                     VEGELAVSVYGGLQATIDPFLHTITIYVRPGCSVEKVLSKLEDEIKRLQEAPPSPDEL
                     ARAVKQARALFAYGSESITNQAFWMGFSEMFASYDWFTGYLDNLAAVTPGDVQRMAQV
                     YLRPQ"
BASE COUNT          818 a          983 c         1016 g          895 t
ORIGIN      
        1 tatgcgcgaa tggatcaacg ggcgaaaccc ggtttacgag gtgctgcgcg ccgcgaggcg
       61 acaagttttc cgtctgcaaa ttgcccaggg tgtccaggaa aagggtcgcc tggctgagat
      121 cgcgagcctg tgccaacggg ggagaatacc cattgaacgc cctcaacgga atcaattaga
      181 tgcgcgcttc cccaacaatc aaggagtggc actcgaagtg agcggctacc cctattcgga
      241 tttaattgat gtgttggctc tggctgaaaa gcgcaaagag ccgcctttgc tgctgatcct
      301 ggacacctta caggatccgc agaacctggg gaccttgctg cgtacggcag agattgttgg
      361 tgttcatggc gtgctgttgc cgctgcggca tacggcgacc gtcactccag cagtagtaca
      421 cgcctcatct ggggctagcg agcatctatt gattactcaa ctcaacttgg cccaggcaat
      481 cgagaccttg aaacgagaag atgtttgggt ggtgggcctg gagggagacc ctgctgaggc
      541 agtgccaagc gatgtcaggc tggatggccc tctggcgctg gtagtaggca gcgaagggga
      601 ggggatgcgc tcattagtgc gcaagtcttg cgatgttttg ttgcgcttgc ccatgcgcgg
      661 ccatgttgag tcactcaacg cagcggtcgc cgggtctgtg gcgctatacc tggcctggca
      721 ggcccgcagg tggggataga aatacaccat ccctataagc agggatggtg atattcagca
      781 gaatggggat tttgatcccc aaattcagtt ggcaagagca ttaggctgca ttcagcctga
      841 ccctatagct gccagcatct cctcattgct accgaacttg aattcatcta gattgcggcg
      901 ctcggcctcc gactgttcga cggcttccag gcgcaggata ttcgccttcg taatgatcgg
      961 cttgctgatt ttctggagaa gtttatttac gttgtccgca actgccgttg gattctccat
     1021 tgcaggcatt gtctgcaaat attgcagcat ggcttgagcg ccgctttctc cgtctttccg
     1081 tgcgactccc accaatcctg cgctggcttc tcgtgaccag ccggcgacga agattccttg
     1141 aatgggttga ccggtttgcg catcaaaaac ttcatgggat atcccattca ccgggtagct
     1201 gggattagga tttttaacaa attcattcca ttgcacaggc agcccgaaag tttcatccac
     1261 cttgtcgccg atacagaaga tgaccgtatc cacatccagg atgcgcttga tcccggtgcc
     1321 cctggctttg gtgtcgccat tgaaggggat gagcttggtc tcttcaactt ccagcccctg
     1381 cacctgtccg ctggcatctc ccaggatgcg cacgggtgaa gccagaaaat caaagagcag
     1441 gcgggattca gagacggatt cataagcttt tggtagcgct gagaggatga agtccttggc
     1501 agcctgaatg tcctgtccaa ccgcctccat tgccggggcg acgcgggcga tctcttcgtc
     1561 caggcctttc aaatccaggt tggaggcaac cgactccatc tctttcttgt cgaatttaac
     1621 ctcagccgga ccacgcctca ctacggcgat gacttcgtcc accttgagat cgcggatcaa
     1681 ccagcgggca atatccagca tcacattgcc cgccccaaca agggctactc gatttccgat
     1741 atgaaagttt tcctggctga atggaggcag tttgttgtag tggtagacga tgtccttggc
     1801 gtggtagaca cccttggata gctcctcacc aggcagacct agccacttgg tgccctgcgc
     1861 gccgactgtc accagaagcg cctgcacgcc ggcatcttta atgtcgtcca gtgtcaggtc
     1921 gccttgcccg ccgatgatga cattgccgta ataatcgata tttggattct gcaggatctg
     1981 gcgaaattgc ttacgcaatc cttctttcat tttgtgtttc gaagggtaaa tgccgtactc
     2041 agccaatccc cctggtttga tatcccggtt gaataagagt acatgggcgc cttgggatgc
     2101 taattcccgc gctccaaaca gaccagccgg tccagcgccg atgacagcga ctacatactt
     2161 attttggatc tcagagctca atgtaattct ccttgatttg tggcttatta gaaatggatg
     2221 atgttccagg aaacgctctt tgattatatt gggttgggcg aaaaatcaca agtgccctcc
     2281 tgcacaatct gagtaaagga agatcatatg gcttcaaata ggataggctg tttttatccc
     2341 aaaaaataaa actgtgctat atttgtgctt tgaacaaatc tatgcctgtt gtgcttcgag
     2401 agggatctta ggggctataa aagaatccgc aactcgttcc aggcgttcac aatgttttgc
     2461 gagaggtaaa agtgggaaca aatttcacga agacgcagct ttctaatgga atacaagtta
     2521 tgctgaaaga aatccatacc gctccaatca tcagtcattg ggtatggtat cgagttggct
     2581 cccggaacga agtggcgggt ttcacggggg tctcgcactg ggttgagcac atgcaattta
     2641 aggggacacc gcaattcccc tccaccgtac tggataaggc gatctcacgc gagggtggtt
     2701 tttggaacgc atttacttac ctggactgga ccacgtactt cgagacaatg cccgctgaga
     2761 aaatcgacct ggccttgcgc ctggaggctg accggatggt taacagccag tttgatccgc
     2821 aagaagtcgc ctctgagcgc acagtgatta tttccgagcg gcagggcaac gagaacgagc
     2881 ccctttttcg gctgggcgag gagctgcagg cattagcttt ccgggtgcat tcctatcacc
     2941 acgaggtgat cggtgatctg gctgacctgg agactatgca gcgcgatgac ctataccggc
     3001 attaccggca atattacact cccaataatg ccctgattgc cgttgccgga gattttgaaa
     3061 gcgaggtgat gctaggacgc atccgagagt tgtttgaact aatccctgct ggtgaggcgc
     3121 tgctccagcg tatccgtccg gagccggccc agtcaggtga gcgccgcttc accctggaag
     3181 gtccgggcga gaccacctac atccagtttg cccaccgtgc cccagcggca tcggaccccg
     3241 atttctttcc gctcactgtg ctggacagcc tgcttaccgg ccctagcagc ctcaatatgt
     3301 tcggaggggg catctcgaac aagacgtcgc gcctttaccg cgccctggta gagggggagc
     3361 tggcggttag cgtgtatggg gggctgcaag caacgataga cccattcctg cataccataa
     3421 caatatacgt acgccctggc tgttcggttg agaaggttct ctcgaagcta gaggatgaga
     3481 tcaagcgctt gcaagaagct cccccttctc ctgacgagct agcccgcgct gtcaagcagg
     3541 cgcgtgccct gtttgcctat ggcagcgaat ccattaccaa ccaggcattc tggatgggat
     3601 tttctgagat gtttgccagt tacgattggt tcacgggata tctggataac ctggcagcgg
     3661 ttacgcctgg cgatgtgcaa cgcatggcgc aggtctatct gcgcccccaa aa
//
LOCUS       DBNGSX010000597         4125 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_758541, whole genome shotgun sequence.
ACCESSION   DBNGSX010000597 DBNGSX010000000
VERSION     DBNGSX010000597.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_758541"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="AC16CB_17320"
     CDS             <1..851
                     /locus_tag="AC16CB_17320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrogen fixation protein NifH"
                     /protein_id="HIR3344285.1"
                     /translation="ILSEMDESGYWAKSGPGYLPKYRSTVWSIIMLAQLGALAEDDPR
                     LERACAYLIDHALASGGQFSASGAPSGTADCLQGNMCAALLDLGYDDPRLEAAFEWMA
                     RTLTGEGIAPNSERDAPVRYYASKCGPLFACGSNNKLSCAWGGVKVMLAFARWPASRR
                     TPLIERAIQQGVDFFFSTDPAKADYPSGFSDKPSGNWWKFGFPVFYVTDLLQIVEALV
                     ALGYAADSRLSNAIELIRQKQDKHGRWALEYSYTGKTWVDFGPKKQPNKWVTLRALRV
                     LKATGD"
     gene            860..1354
                     /locus_tag="AC16CB_17325"
     CDS             860..1354
                     /locus_tag="AC16CB_17325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HIR3344286.1"
                     /translation="MKNSSQPPKLIWRVMRKLNLRIARNYLRGIGPGSIVLLLTTTGR
                     KSGLPRLTPLQYEEYEGMYYIGSARGQQADWFRNIQANPQVEVQVRRKRFPGLAEPIT
                     DPKRIADFLEMRLRRHPLMIGALMLLEGLPLRHTRADLERIAAQKALVIIRPILGSAS
                     STDR"
     gene            complement(1470..1643)
                     /locus_tag="AC16CB_17330"
     CDS             complement(1470..1643)
                     /locus_tag="AC16CB_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HIR3344287.1"
                     /translation="MAKDPVCGMDVDPKTAQWRSEYKGQMYYFCAPGCKRSFEKDPEK
                     FLSKLGDPAHHVH"
     gene            complement(1766..2215)
                     /locus_tag="AC16CB_17335"
     CDS             complement(1766..2215)
                     /locus_tag="AC16CB_17335"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3344288.1"
                     /translation="MKRVFYLLVLLCLIALLSACGGGSSQNADAVQQGEVAGEKVKVA
                     GGAYTDVDVAELQSMLANKDFTFVNVHIPFEGDIANTDVSIRYDQIEQNLSLLPPEKD
                     AKIVLYCRSDRMSNIAAETLVSLGYSNVYNLKGGMVAWERAGLPLVK"
     gene            complement(2307..4034)
                     /locus_tag="AC16CB_17340"
     CDS             complement(2307..4034)
                     /locus_tag="AC16CB_17340"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3344289.1"
                     /translation="MVETNPRPYSPWRGLTLQLFVLTILPLTVLLLFITVNSLSVHQR
                     AMRALVGERDERAVRTASRALESEIFHRASAVRSLALRAGNADSTALDYILSSSAFLQ
                     ADFDGGLAFFDQQGRLLSFTGETSLWENMASQGTTWIEAVLSSDAFPAYISPTFRDQI
                     SSEAMVLVAASAESTEIIAVGVFSPGSLVGRALEGAFASESQIGILLVDASGNALYES
                     GSLSSGEGIGAYSGVEEALRGKAGTIYIGSGDDEHVVAYAPVEPVGWAIVTEESWELV
                     TTPTLRTSQVMPLALVPALLIAVVGLWFGIRQVVQPIQKLEEKAARLAWGDFGSIEEP
                     VGGIPEVQHLQVELAQMARKLRAAQKGLHDYIGAITAAQEEERRRLARELHDDTIQSL
                     IALKQRVQLTRLSIKERQAADSMAELEGLAEQTIDNLRRLTRALRPIYLEDLGLVTAL
                     EMLARETSQSAGIPVTFHLDGSERRLPPEVEMAFYRITQEALSNVIRHSKASEASVRL
                     DYQPVSVSVEISDNGLGFQVPRSPAEFVPRGHFGLLGLHERAELLGARLEIRSAPGKG
                     ASIIVDMPT"
BASE COUNT          928 a         1210 c         1088 g          899 t
ORIGIN      
        1 ccatcctatc cgagatggat gagagcggat actgggcaaa gtcaggccct ggttatctcc
       61 ccaaatatcg ctctacagtc tggtcaatca tcatgctggc gcagttgggc gcgctagccg
      121 aagatgaccc gcgcctggaa cgcgcctgtg cctacctgat cgatcatgcc ctggcatctg
      181 gcgggcaatt ttccgccagc ggggcgcctt ctggcactgc tgactgtctg caaggcaaca
      241 tgtgtgctgc cctgctagac ctgggctacg acgacccgcg gctggaggcg gcttttgaat
      301 ggatggcgcg caccctgacc ggcgaaggta ttgccccgaa ctccgagcgc gatgcccctg
      361 tgcgctatta tgctagcaag tgcggaccgc ttttcgcctg cggctcgaac aacaagctgt
      421 cctgcgcttg gggtggggtc aaggtcatgc tggccttcgc tcgctggccg gccagccgcc
      481 gcacgcccct catcgagcga gccatccagc agggtgttga ctttttcttc agcaccgacc
      541 cagccaaagc cgattatcca tccggattca gcgataaacc cagcggcaac tggtggaagt
      601 ttggcttccc ggtattctac gtcaccgact tgctgcaaat tgtggaagcg ctcgtcgctt
      661 tgggctacgc ggctgacagc cgcttgtcaa atgccataga gctcatccgc cagaaacaag
      721 acaagcacgg gcgctgggcg ctggaataca gctacaccgg caagacctgg gtcgactttg
      781 gtccgaagaa gcaacccaat aaatgggtga ccttgcgcgc tttgcgcgtc ttgaaggcaa
      841 caggggatta aatgccttaa tgaagaattc gagccaaccc ccaaagctga tctggcgcgt
      901 gatgcgtaag ctgaacctgc gcatagcacg aaactacctg cgaggcattg gcccaggtag
      961 catagtgctg ctgctcacca ccaccggcag gaaatcaggc ttgccgcgcc tgacgcctct
     1021 ccagtacgag gaatatgagg ggatgtatta cattggttca gcgcgaggcc agcaagctga
     1081 ctggttccgc aatatccagg caaacccgca ggtcgaggtg caggtgcgcc gcaagcgctt
     1141 ccctgggctg gcggagccga tcactgatcc gaaacgcatc gccgactttt tagaaatgcg
     1201 cctgagacgc caccccttga tgatcggcgc tctgatgctc ctggaagggc tgccgctcag
     1261 gcacacccgt gctgatttag agcgcattgc ggcgcaaaaa gcgctggtca tcatacgacc
     1321 gattcttggt tcggcgtcat ctacagacag gtagcgaata aaagtcacat agtgggggaa
     1381 gtaatcgatg cagttaggca agggaataca agccaaaatt tgatagtctg ggaggcttcg
     1441 ctgctcaatc tatcaaattt tggctatgtt tagtgaacgt ggtgggccgg atctccaagc
     1501 ttgctcagga atttttcggg gtctttttca aatgaccgct tgcagcccgg ggcacagaaa
     1561 taatacatct ggcccttgta ttccgatctc cattgagcgg tcttggggtc gacgtccatt
     1621 ccacatacgg ggtctttagc cattggcgca atctcctttc tagctgactt ctattgacct
     1681 acagaatttt tggttatgaa cgtcccgcag gtttaggatc acacaggctt gtttcagggg
     1741 aacctgcggg acgtttttac atagatcact tcaccagagg caggccggct ctctcccagg
     1801 ctaccatacc accctttaag ttataaacgt tgctataccc caaactaacc agcgtctcgg
     1861 cagcaatgtt gctcatccgg tcgctgcggc agtacaagac gattttggcg tccttctcgg
     1921 ggggcaacag agatagattc tgctcgatct ggtcataacg gatggataca tcggtatttg
     1981 caatgtcccc ttcgaagggg atgtgcacat tgacaaatgt aaaatccttg ttcgccagca
     2041 tggattgcaa ttcggcgaca tcaacatcgg tgtaggcacc gccggcaacc ttcaccttct
     2101 cacccgcgac ctccccctgc tgtacagcgt ccgcattctg tgatgatccc cctccacagg
     2161 ctgaaagcaa ggcaatcaaa cacagcaaaa ccaaaaggta aaatactctc ttcatcgatc
     2221 atttactcct tctacaaatc tagatctcat cctgatatgc ttgcttgagg gttttggcac
     2281 cctcaatttt tttaactgaa cgatacttat gtgggcatat ccacgataat gctggctccc
     2341 tttccaggcg cggaacggat ctccagcctg gctcccagca attcagcgcg ctcatgcaac
     2401 cccaacaatc caaagtgccc gcgcgggaca aactccgctg ggctgcgggg cacctggaat
     2461 cccaggccat tatcacttat ctccacagac acagagactg gttgataatc gagccgtacg
     2521 ctcgcttcgc tggccttgga atgacggatc acgttgctca aagcttcctg ggttatccgg
     2581 tagaaggcca tctccacttc tggaggcagc cttcgttcgc tgccgtccaa atggaatgtc
     2641 actgggattc cagcgctctg gctcgtctct cgagccagca tctccagagc agtcaccaaa
     2701 cccaagtcct ccaagtatat tgggcgcagg gcacgcgtca atcggcgcag gttatcgatg
     2761 gtttgctcag ccagcccctc aagctccgcc attgagtcag cagcctgccg ctccttaatg
     2821 gacagtcgcg ttagctggac gcgttgcttg agtgcgatta aggactggat agtatcatcg
     2881 tggagctccc gcgccaggcg gcggcgctcc tcttcctggg cagctgtgat agcgccaata
     2941 taatcgtgca agcccttttg ggcggcgcgc agtttgcggg ccatttgagc cagctcaacc
     3001 tgcaggtgct gcacttctgg gatgccgccg accggttcct caatggagcc aaaatctccc
     3061 caggcgagcc gggcggcttt ttcttccagc ttttgaatcg gctgaaccac ctgccgtatg
     3121 ccaaaccaca agccaaccac agcaatcaac aaggctggca ccagggccag gggcataacc
     3181 tgcgatgtgc gcagggtagg ggtagtcacc aactcccacg actcttcggt gacgatagcc
     3241 cagcccacag gctctactgg tgcataggcg accacgtgct cgtcatcccc agatccgata
     3301 tagattgtcc ccgccttgcc gcgcagtgcc tcttctacac ctgaatatgc acctattccc
     3361 tcgccagatg ataacgatcc gctctcatac agcgcattac cactggcatc caccaaaagg
     3421 atgccgatct ggctttccga ggcaaaggct ccctccagag cgcgtcctac cagcgaacca
     3481 ggagagaaga ctcccactgc aatgatctcg gtggattcgg cactcgctgc aaccaggacc
     3541 atcgcttcac tgctgatttg atcgcggaat gtgggtgaga tgtaggcagg gaaagcatcg
     3601 gacgagagca ccgcttcgat ccaggtcgtc ccttgagatg ccatgttctc ccacagactg
     3661 gtctccccag taaatgacag caacctcccc tgctggtcaa aaaatgccaa tcctccatca
     3721 aaatcagcct ggagaaaggc gctgctggat agaatataat ccagtgcagt ggagtcggcg
     3781 tttccagccc gcagagcaag gctgcgcact gcggatgcgc ggtgaaaaat ttcagactct
     3841 agagcgcgcg aagccgtgcg tacggcgcgc tcgtcccgct caccgaccag ggcgcgcata
     3901 gcgcgttgat gcaccgacag actgttgacc gtgatgaaca ataacagcac agttagcggc
     3961 aggatggtta acacaaataa ttgcaatgtc agcccgcgcc agggagagta aggccgaggg
     4021 tttgtttcca ccatgcaagg tacctataag ctaatcgtct ttgatctccc caatggattg
     4081 ggagatccac cctaaggaaa ccgcacgcat aaccgcctcg gtacg
//
LOCUS       DBNGSX010000598         7236 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_481714, whole genome shotgun sequence.
ACCESSION   DBNGSX010000598 DBNGSX010000000
VERSION     DBNGSX010000598.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7236)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7236)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7236
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_481714"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="AC16CB_17345"
     CDS             <1..789
                     /locus_tag="AC16CB_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase-like TK C-terminal-containing
                     protein"
                     /protein_id="HIR3344290.1"
                     /translation="HFGVREHAMGSIVNGMALHGGVIPYGGTFLVFSDYMRPAIRLSA
                     LSDFPSIWVFTHDSIGVGEDGPTHQPVEHLAALRSIPKLVVVRPADANEAREAWKIAI
                     SRRAGPTALIFTRQNLPIIDRNIYGSASGVQQGAYVLADLGDGAPELILMASGSEVSL
                     IVQAGIILAAEGLNVRLVSFPSWELFNMQTKEYRDSVFPSNIRSRLAVEAGVSQGWER
                     WVGDQGIVLSIERFGASAPYQVIYEKYGLTVENIVTYARTMVSR"
     gene            812..1504
                     /locus_tag="AC16CB_17350"
     CDS             812..1504
                     /locus_tag="AC16CB_17350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344291.1"
                     /translation="MEYDNLHKSDTQPRLFSLGQASSGIAELFPEIWTASEDLSSPNP
                     TIRRLALEFLEHSGAARISPLVVYIIATRLDDTDLVVRMQVLKILGDVLAPDELGNSP
                     PEMVTSYLTNNLSNMRTRQVYAILQALVQDINLDLQVIRILKTCPYAGNHLMEMANSR
                     KVPLNMRRKAIWLVGQIGYLEAIPALERMQIRMETRLTGQQSMPFAPPIGIDDIDLLD
                     DVISTLGLLQSP"
     gene            1517..3022
                     /gene="zwf"
                     /locus_tag="AC16CB_17355"
     CDS             1517..3022
                     /gene="zwf"
                     /locus_tag="AC16CB_17355"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HIR3344292.1"
                     /translation="MFDSEKVEKAAPTAFIIFGVTGDLTKRKLVPALYELALTQRLPD
                     AFYIIGFARRDWDNLTMAKTLTQNVSDFARTNTVDTKVLEALCSKMRYVQSTFEEEDG
                     YRRLGQILDELGIINRLYYLAAPPEDYVNIVRLLGNAQLCKSERGWTRIVIEKPYGRD
                     LDSAMRLDEEVHRVFKENQVYRIDHYLGKETVQNLLVFRFANGIFEPLWNRENVDHVQ
                     ITVAESVGVGSRAGYYERSGVIRDMFQNHMLQLLALTAMEAPVAFNADSVRDEKVKII
                     KSLRPLEGIEALANTYRAQYAAGTVDGKRVPGYKDEPGVSPESITETYLALRLFVDNW
                     RWAGVPFYLRSGKRLPRRVTEVSVHFKQVPLPLFGWRNLAGDAPNILTINIQPDEGIA
                     LTFGAKAPGPANQILPVEMQFDYVKSFGGEPPEAYERLLLDSLSGDATLFTRTDEVHT
                     AWSFTTQIIDSWNANPVRNLPVYEAGTWGPPGADEFVTASDGHSWHNTGDS"
     gene            complement(3019..3606)
                     /locus_tag="AC16CB_17360"
     CDS             complement(3019..3606)
                     /locus_tag="AC16CB_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR3344293.1"
                     /translation="MEPRIFHGDITTQYFASHLLSQFNRGNFRAQQVGGGNLVAVQIM
                     TRERPTSGGETALTVNLQKVEDGVSVQIGKQAWLGVAASLGVTALTALRNPLAFFSRI
                     DDLAQDIENLQLSEQVWDTIEAAAKAANASFELSERLRRLVCEYCLTANPIGEPSCIA
                     CGAPLGISQPQTCNNCGFVIKRAEVNCPNCGQKLD"
     gene            3722..4348
                     /locus_tag="AC16CB_17365"
     CDS             3722..4348
                     /locus_tag="AC16CB_17365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR3344294.1"
                     /translation="MRKNIRLWLIAFGFLLVIILLGGVASVYASELGQIQIPTVSVPT
                     VTSSPVGAFVTVTRDFDQVNVRGGPGGDGAYPVVGVLIAGQQVPALGRSEGGLWIQIA
                     YPGAPGGVAWVYTPLVEVSGSLPIVEPPPTPTPRTTPTIDPTLAAQFIIEIPPTHLPT
                     FTPPPPLSIPTFSADVVPATERVPMGLVIIGMGLIGMFGLLISLLRGR"
     gene            complement(4416..4814)
                     /gene="erpA"
                     /locus_tag="AC16CB_17370"
     CDS             complement(4416..4814)
                     /gene="erpA"
                     /locus_tag="AC16CB_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HIR3344295.1"
                     /translation="MLADQVHTGTITLTPTAAQAVVDLLNKRNLTDYALRVFISGGGC
                     SGYQYGMALENQIRETDLEIEMHGVKVVVDEISIDYLRGCTIDYVDDVMGSGFKIDNP
                     NAVSSCGCGSSFRTKNSSEAHDSGGGCGCG"
     gene            4939..6102
                     /locus_tag="AC16CB_17375"
     CDS             4939..6102
                     /locus_tag="AC16CB_17375"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR3344296.1"
                     /translation="MEFKRSFLITFILGICLTLGFAGGYYAHAHFDLWRDQFPVLNQA
                     FDILGDNGLKPMPEKPALEYGMIRGMLQAYDDPYTLFVEPPQHELESNALQGSFGGIG
                     VRLTRDLEGYVILYPFQGSPASEMGIMDGDRLIFVDDMEISPEVTIESVQAALRGPVG
                     QSVRVTISRPPDNGQLEFIIRRAEIPLPSVAWHIDSDIPEVGVIELNIIAASTVQEVQ
                     SAIRDLQQRGAQYFILDLRDNFGGLLTAGVDVARLFLDNGVIMEYENRDKKIEVYRVE
                     QPGEFVDVPLVVIVNENTASASEIIAGALKAHQRAQIVGTHTYGKNTIQLVFDLMDGS
                     SLHVTSAQWWIPGLEPPIGKGGLEPDIYTETTPDEAGVDAAIREASLVLMRLK"
     gene            6195..7037
                     /locus_tag="AC16CB_17380"
     CDS             6195..7037
                     /locus_tag="AC16CB_17380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR3344297.1"
                     /translation="MKAKKRRVGCGCGSFFGGLLLALVIILTIYLFAPLRTNLLLLGI
                     DYAPPGSSVSRSDTIILATVIPTEPYIGVLSVPRDLWVNIPGIGENRVNTAHFFAEAQ
                     QVGSGPKAAIDTIQQNFGVDVDYFVRIRFDGVRSVVDAMGGVDIDLDRPMAGYPAGRH
                     HLTGNKALAFARHRLGADDFFRMEQGQLLIKAIFRQLLHPSMWPRLPSVVVAIMNEVD
                     TNIPLWQWPRLALALLRAGPDGLDNRTLERDMTTPFTTNQGASVLLPDWSKINPLIED
                     MFGY"
     gene            7111..>7236
                     /locus_tag="AC16CB_17385"
     CDS             7111..>7236
                     /locus_tag="AC16CB_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HIR3344298.1"
                     /translation="MGIKIVAKNRKASHEYFLLDTYEAGLVLQGSEIKSIRAGQIS"
BASE COUNT         1844 a         1606 c         1841 g         1945 t
ORIGIN      
        1 cactttgggg tccgtgagca tgccatgggg tccattgtta atggcatggc tttgcatggg
       61 ggagtgatac catatggcgg tacattcctc gttttttctg attatatgcg cccagccatc
      121 cgcttatctg ctctgtctga cttccctagc atttgggtat ttacacatga tagtattgga
      181 gtgggtgaag acgggcctac gcaccaacca gtggagcatc ttgcggcatt aaggagcatc
      241 ccaaagctgg ttgtggttcg tccagcagat gcgaatgagg cgagggaagc ctggaaaata
      301 gctatttcac ggcgggctgg acccacggca ttgattttta cgcgtcaaaa cctgccaata
      361 attgatagaa atatatacgg ttccgcaagc ggtgtgcagc agggggcata cgtattggct
      421 gatctgggag atggagctcc tgaattaatt ttgatggctt cgggatccga ggtatcatta
      481 atagtccagg ctggtataat cctggctgcc gaaggcttga acgtcaggtt ggtatcattc
      541 cctagctggg aactttttaa catgcaaacg aaagaatacc gtgattcggt tttcccttcc
      601 aatatacgtt cacggctggc tgtcgaggcg ggtgtctcac agggttggga gcgctgggtt
      661 ggagaccagg gcatagtcct ctcgattgag aggtttggtg cctcagctcc gtatcaagtc
      721 atttatgaga aatatgggtt aacggtagaa aatattgtga cctatgccag aacaatggtc
      781 tccagataag tctcggggaa ggtagctatc gatggaatat gataatctgc ataaatccga
      841 tacacaaccc aggttgttta gcctaggcca agcaagctct ggaatagccg aattatttcc
      901 ggaaatatgg acagcatcgg aagatctatc cagcccaaat cctactattc gccgtctagc
      961 tttggaattc ttagagcatt ccggtgcagc gcggatttct cctcttgtgg tttacatcat
     1021 tgcaactcgc ctggatgaca ctgaccttgt agtgagaatg caggtgttga aaatactggg
     1081 agatgtgctt gccccggatg aattgggaaa cagtccgcca gagatggtca ccagctatct
     1141 cacaaacaat ctgagcaata tgcgcacccg ccaggtatac gcgattttgc aagcattggt
     1201 gcaagatatc aatttagatt tgcaggttat ccgtattcta aaaacctgcc catatgctgg
     1261 taatcatctg atggaaatgg caaattcacg caaggtacca ctgaatatgc gccgtaaagc
     1321 catatggttg gttggtcaaa taggctattt ggaggcaatt cctgcattgg aacggatgca
     1381 aatccgaatg gagacacgcc taaccggtca gcaaagcatg ccatttgcgc ctccgattgg
     1441 aattgatgac atcgacctat tagacgatgt gatttccact ttaggattgc tccaatctcc
     1501 ttaattctca ttacccatgt tcgattctga aaaagtcgaa aaagcagctc caactgcatt
     1561 tattatcttt ggtgtaacag gtgatcttac caagaggaag cttgtgccag cgctctatga
     1621 gcttgcactt acccagcggc ttccagatgc tttttacatc attggatttg cgcgtcgtga
     1681 ttgggacaat ttgaccatgg caaaaacgct cacccaaaat gtgagcgatt ttgcgcgtac
     1741 aaatacggta gatacaaaag tcctggaagc tttatgctcc aagatgcgct atgtccaatc
     1801 gacattcgaa gaagaggatg gctacaggcg gcttggccaa atattggacg agttggggat
     1861 tatcaatcga ttatattatt tggctgctcc tccagaggac tatgtaaaca ttgtccgatt
     1921 attgggcaat gcccaattgt gcaagtctga acgtgggtgg acgcgaatcg tcatagagaa
     1981 accctatgga agagatctgg attctgcaat gaggttagat gaggaggttc accgagtctt
     2041 taaagaaaac caggtttacc gaattgatca ttatctagga aaagagacgg ttcagaattt
     2101 actcgtcttc cgctttgcaa acggcatctt tgaaccgttg tggaaccggg aaaacgtgga
     2161 ccacgtccaa attacagtag ccgaatccgt tggagttggg agcagagctg gatattacga
     2221 acgctcgggt gtaattcggg atatgttcca gaatcacatg cttcagctgc tagctctgac
     2281 agcgatggag gcaccggtag cctttaatgc ggattctgtc agagatgaga aagtcaaaat
     2341 aattaaatcc ttgcggcctt tagaaggaat agaggctctg gcaaacacct atcgcgccca
     2401 atatgctgct ggtacagttg atggtaagcg agtacctggg tacaaagatg aacctggggt
     2461 ttctccggaa tcgattactg agacatactt agctttgcgc ctatttgtgg ataattggag
     2521 atgggctggt gtgccctttt atctacgctc gggtaaacgc ttgccacgac gggttactga
     2581 agtttctgtt cattttaaac aagtgcccct gccgctcttt gggtggcgaa acctggcggg
     2641 ggatgcaccg aatattttga caatcaatat ccagccggac gaagggattg cgctgacctt
     2701 tggcgcgaag gcaccaggac ctgcaaacca aattttaccg gttgagatgc aatttgatta
     2761 tgtaaagtct tttgggggcg agccgcctga agcttacgag cgcttgctgc ttgacagtct
     2821 atctggggat gccactcttt ttacccggac tgacgaagtt cataccgctt ggtcttttac
     2881 tactcaaatt atcgatagtt ggaatgctaa tccggtaaga aacctaccgg tgtacgaggc
     2941 cggtacctgg ggcccaccgg gagcagatga gttcgtaaca gcctcagatg gtcatagctg
     3001 gcataatact ggggatagtt aatctagctt ttgaccgcag tttgggcaat ttacttcagc
     3061 gcgtttgatc acaaagccac aattattaca ggtttggggt tgggatattc ctaacggagc
     3121 gccacacgca atgcagctgg gctcacctat cggattggca gtcaaacaat actcacaaac
     3181 caatcgacgg agcctctcgg agagctcaaa agatgcattt gccgcttttg ctgcagcttc
     3241 aattgtatcc caaacctgtt cgcttagttg gagattctcg atgtcttgtg cgaggtcatc
     3301 gatgcgggaa aaaaaagcta gagggttgcg caatgcggtt aaagcagtta cacccaggct
     3361 ggcagctaca cctaaccagg cttgcttgcc aatttgaacg gataccccat cttccacctt
     3421 ctgcagattg accgtcaagg ctgtttcgcc accagaggtt ggtctctccc tagtcattat
     3481 ttggacagca acgagattgc cgccccctac ctgttgggct cgaaagttgc cgcggttgaa
     3541 ttgcgatagc agatggctgg caaaatattg tgttgtgata tcgccgtgaa atatgcgagg
     3601 ttccatttta cttactcttt cttcataatc tgtagtgaag ggttcgtgtg atgatcattg
     3661 cctgtggctt cgagtagttc tgcattagat tataatcggt acgaaataat cgggagaaag
     3721 aatgcgcaaa aatattaggc tttggctcat agcctttggt ttcttgcttg taattattct
     3781 acttgggggt gtggcaagcg tgtacgctag cgaattggga caaatacaaa tcccaacggt
     3841 ttccgtacca accgtcacca gcagcccggt tggtgcgttt gtgacggtta cccgagattt
     3901 cgatcaagtt aatgtacgcg gaggacctgg tggagatggc gcttaccctg tggttggagt
     3961 gctaatcgcc ggtcaacagg ttccggcatt aggccgctct gagggcgggt tgtggatcca
     4021 aattgcatat ccaggcgcgc ctggcggagt tgcctgggta tatactcctc tcgtagaagt
     4081 tagcggttca cttccgattg tagaaccacc tccaacgcct acacctagga caacccccac
     4141 aatagaccca accctggcag cgcaatttat tattgaaata cctccgacac acttgcccac
     4201 atttactccg ccacccccgc tttcgattcc cactttttct gcggatgtag tcccagccac
     4261 cgagagggtg ccgatggggt tggttatcat tggcatgggg ctaattggga tgtttggctt
     4321 gctaatctct ttgttgcgag ggcgataaaa ttgatcattg agaaaaaaag cgggcgatgt
     4381 gatcgcccgc tcatttatca tatgattagc gtggattatc cacagccaca accgccacct
     4441 gaatcatggg cttcagatga attttttgtc cggaaggagc tgccacaacc acacgaagaa
     4501 acggcattgg gattatcgat tttaaacccg ctgcccatca cgtcatcaac ataatcgatt
     4561 gtacaaccac gtaaataatc gattgagatc tcatccacaa caacttttac gccgtgcatc
     4621 tcaatttcca aatcggtctc tctaatctga ttttccagtg ccatcccata ctgataacct
     4681 gaacaacctc caccagagat gaatacacgg agtgcatagt ctgtgagatt gcgcttattt
     4741 agtaaatcca ctacggcttg agcagctgtg ggggttagtg taatggtgcc ggtgtgtacc
     4801 tggtctgcca acataactta atctctccta catcatcgta aggcaatgat gctcaatttt
     4861 ggtaaatttt atcaagatta acgaaaccag tcaatagtac ctcaaaaggc ataaaaggtt
     4921 ataatcccag attgttctat ggaattcaaa cgctctttct tgataacttt cattctagga
     4981 atttgtctaa ccctcggctt tgctggaggc tactatgccc atgcacattt tgatctatgg
     5041 agggatcaat tcccggtctt gaatcaagcc ttcgatatct tgggtgataa tggattaaaa
     5101 cccatgccgg aaaaacctgc cttagagtat ggcatgatcc gtgggatgtt gcaggcatat
     5161 gatgatccat ataccttgtt tgttgagccg cctcagcatg aactggaaag taacgcattg
     5221 cagggtagct ttggaggtat tggtgttaga ttgactcgcg atctggaagg atatgtcatt
     5281 ttatatccat tccagggaag tcctgccagc gagatgggta ttatggatgg tgatcggctt
     5341 atcttcgtag atgatatgga gatatcgccg gaagttacaa ttgaaagcgt ccaggcggcg
     5401 ctcaggggac cagttggaca aagcgtgcgt gtaaccatat cacgcccgcc cgataatggg
     5461 cagctcgaat ttatcatccg acgcgcggaa attccattgc catcggttgc ctggcatata
     5521 gactctgata ttccagaagt tggggtgatc gagctaaaca tcatcgctgc cagtactgtg
     5581 caggaggttc aatccgcgat acgcgatttg caacagcgag gtgcgcaata tttcatcctc
     5641 gacctgcgag acaattttgg gggtttgctt acagcaggag tggatgtcgc cagactgttc
     5701 ttggataatg gggtcattat ggaatacgag aatcgcgata aaaagattga ggtttatcgc
     5761 gtggagcagc caggagaatt tgttgatgtg ccgcttgtgg tgattgtcaa tgagaatacc
     5821 gctagcgctt cggaaataat tgctggggcg ttgaaagcgc atcagcgtgc ccagattgtt
     5881 ggcacgcaca catatggaaa gaatacaatc caattagtgt tcgacctgat ggatggctcc
     5941 agcctgcatg taacctcagc gcagtggtgg ataccgggcc ttgaaccgcc tattggaaaa
     6001 ggtggtctgg agccggatat ctatacagaa accactccag atgaagctgg agtggatgca
     6061 gctattcggg aggcatcctt ggtcttaatg aggttaaagt aatgtcacac gaaaattggg
     6121 tcgaccccca atctcagcca aatgcatatt atcctccccc gatgccccca tcctactcaa
     6181 gcccgccaaa atggatgaaa gcgaaaaaaa gacgggttgg gtgcggatgt gggtcttttt
     6241 tcggagggtt gctgcttgca ttggtcataa tactaacaat atatttgttt gcacccctgc
     6301 gaacgaattt attgctttta ggtattgatt atgctccccc tggtagctca gtcagccgga
     6361 gcgacacaat tatcttggcg acagttatcc caacagaacc atatattggc gtgttgtcgg
     6421 tcccacgaga tttgtgggta aatattccag gcataggtga aaatcgcgtc aacaccgccc
     6481 acttcttcgc ggaggcgcag caggtaggca gtggaccaaa agctgccatt gataccatac
     6541 agcagaattt cggtgttgat gtagattact ttgtgcgcat ccgttttgat ggtgttcgca
     6601 gtgtggttga tgcaatggga ggtgtggata ttgatcttga tcgcccaatg gctggctatc
     6661 ctgcaggtcg ccatcatctg acaggcaata aggcccttgc ttttgcgcgg caccggcttg
     6721 gcgcggacga tttcttccgc atggagcaag gccaactatt gatcaaagcg atatttcggc
     6781 aactgctgca cccttctatg tggcctaggt tgcccagcgt agttgtggcg atcatgaatg
     6841 aagttgacac gaatatccca ttgtggcagt ggcctcgtct tgccctagcg ttattgcgcg
     6901 caggaccgga tggcttggat aaccgaacct tagaacgtga tatgacgaca ccttttacga
     6961 caaatcaggg ggcgagtgtt ctgctgccag attggtcaaa aatcaatcca ctaatcgaag
     7021 atatgtttgg ttattgattt agacattgtg gagctggaga acatctccca aactacttat
     7081 atcatgtggt tatgttaaaa tttgattgca atgggaataa aaatagttgc caaaaatcga
     7141 aaagccagcc atgagtattt tttattggat acgtacgagg ctggtttggt gctgcaaggg
     7201 agtgaaatta aatctatacg cgccggtcaa attagc
//
LOCUS       DBNGSX010000599         8923 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_620262, whole genome shotgun sequence.
ACCESSION   DBNGSX010000599 DBNGSX010000000
VERSION     DBNGSX010000599.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8923)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8923)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8923
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_620262"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..2915
                     /gene="gyrA"
                     /locus_tag="AC16CB_17390"
     CDS             258..2915
                     /gene="gyrA"
                     /locus_tag="AC16CB_17390"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR3344299.1"
                     /translation="MELGLVRQIDIDAEMQQAYLDYAMSVIVARALPDARDGLKPVHR
                     RIMYAMYDMGLRSDSAYKKSARIVGEVLGKYHPHGDMAVYEAMARMAQDFSMRCLLVD
                     GQGNFGSVDGDPPAAMRYTEARLASPAQHMLADIQKNTVDFEPNFDDTLNEPTVLPAA
                     IPNLLVNGATGIAVGMSTSIPPHNLSEVIDALKYLLGNWERLDDIDLEDLMRFIQGPD
                     FPTGGVIIQEGGEETLATAYGSGRGRVMVQARAHLEEMERGRNRIIVTELPYMTNKST
                     LIERIAELARDGKLEGLADLRDESDRQGMRIVIELNKAADPETVLRDLYKYTPMQSTF
                     SIIMLALVEGEPRMLTLKQALRFYLEHRLEVIRRRSQYDLDKARQRAHLLEGLRIALK
                     NLDEIIGLIRKAPDVETARGRLIRRFKLSDLQAQAILDMQLRRLAALERKKIEEEYKE
                     ILAQIKELEGLLRSPKKMRQLCADELQAVKDAYGDRRRTQIVQLEAGREKRSLLTIEN
                     LAPEKTSWLVVTTDGLLSRTLEDKVPRQSGNDAPNWLLRVNTRDTLYLVCEQGETAAV
                     PVLAIPEADKPSEGVIYSKVSALKDENKLAVLFTLPPKGERADDWYVITATRKGMVKK
                     SAINDLPGTSANIFTLVKVNEGDRLGWLQLTNGGKEVLLVTALGMAIRFNEDEVRPMG
                     LVAAGVMGMKLQSKDEVAGMEILPQPGEVFIAVSDGSAKRVAPAQFPKQGRYGQGVVA
                     WKLPGKARVIGLAVGKGTARVTLHLSKLAPKMSRLDEAPLQSRVARGARIQELKPGEQ
                     VLRLTVPWDVERPIAGVKAKVGKRVRRSTAESTSSRSAKPAPRKRGPAKASQAEAKPV
                     KSRSKKPSTRSGSAKPAPAKRTQRRSGPK"
     gene            complement(2938..4041)
                     /locus_tag="AC16CB_17395"
     CDS             complement(2938..4041)
                     /locus_tag="AC16CB_17395"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3344300.1"
                     /translation="MPLKGKGFFIWQIRNTEGGNASAIASLADQANLSHVLIKIADGT
                     YSYNVNSSGVDLVPPVAQALRARGIQVWGWHYVYGDSPIDEANKAIQRIQQTNMQGYV
                     IDAEGEYKQPGKDKAAAQFMSRLRSALPNFPIALCSYRYPSYHPQLPWKEFLEKCTYN
                     MPQVYWVSAHNPGDQLRRSVSEFQAMTPFRPIIPVGSAYKSGSWAATASDVTEFLRTA
                     QSLNLSAANFWEWANCRTYLPSVWNAIRDYKWDAEPPPPPPTDITEQYITALNTHNPA
                     QVVGLYTPAAVHVTAARTIQGTVAIQNWYQTLFTQLLPNASFTLVSFSGSGSSRHFTW
                     TATSSAGKVNNGSDTLGLIEGKIAYHFSSFSIT"
     gene            complement(4043..5116)
                     /locus_tag="AC16CB_17400"
     CDS             complement(4043..5116)
                     /locus_tag="AC16CB_17400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR3344301.1"
                     /translation="MSTMKRLLILALLASTLSACFMPGAIGMPNSLPVLPPTETARPP
                     LLEAPPDATATPTPFQPIPPTAAFEPTEIPTETPIPTPEVLTFESLPTDFGPVGSIEE
                     PKGITNILLLGSDMRSWWGKGSLFRTDTIILASLNPEAGTVSLVSFPRDLYITIPGHG
                     QDRINTAWGRGGFSKLSAAFEYNFGVQVDHYVLIEFSNFKRVVDSLGGLDVQVGERLS
                     DYYRGRLTTIKPGMRTMNADMVLWYVRSRKTSNDFARNRRQQEILQALINKFLSLDAI
                     QRAPELYDLYDESVKTDLNLFDLLPYLPLAAKLGDGKRVKNYFVGSGKVTPYVTAGGA
                     QVLLPQKDKIMRLLRKAMDGQAE"
     gene            complement(5133..6050)
                     /locus_tag="AC16CB_17405"
     CDS             complement(5133..6050)
                     /locus_tag="AC16CB_17405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR3344302.1"
                     /translation="MSSLRQVALGILTALFSIALVFGSVLIALTEGGVHLAMLPTPSQ
                     TAAPLVATPKPGEATFTPTLSPTPTETPAQPSSACKDTPAGWLSREVLPGETLDSIAA
                     EYGISVEILRQANCLTLDTLLPRSLISVPPPTQTSTPTATSTLRPTAAEQKKTSKQAT
                     RAPSRCSGHPARWVAYKVKRGDTIFRIAISYGLSSRELMVANCLTSDVIRVGQIIYVP
                     GYPPITPKPTATPKRTATSVPVATEPPVLTTESPPQITEPPADTTEPPSDTTEPVPVQ
                     TQSQPVRTEPPPVVTEFVPIRTEPPIPYP"
     gene            complement(6047..8437)
                     /locus_tag="AC16CB_17410"
     CDS             complement(6047..8437)
                     /locus_tag="AC16CB_17410"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3344303.1"
                     /translation="MDTFLQELLTLLTQSPGNLTYHVVLSFSVAGSLQASLSQWRNNQ
                     FPQGRRLIIGLSLLLAIRLVIFVLAGIAWQGLVNEHILLPPVDRAATLISLLIIIWMW
                     CIPEPLRLADAATWLLGLLGLTGLALNLVWWLGQGSGIDYNGSWPDFIGEVVAISLIV
                     IGGVILLVRKPNSWGYGLGMLGIALAGHVAYLALPAPEGDYSGVLRMAQMAYYPFLFA
                     LPQRFPTSTQQVPIPAVPAEHKRPTSDPKLLESFLYLSSESEPDRICQAITRTLATAL
                     VADICLLLTPPNEQNEINIQCGFDLIREENLTGSSLSAKSVSLISTALTRRLPLRLPA
                     SSTSTDLRTLASHLHLERPGHMLFVPVVSADDSPIAGLLLLSPYSNRAWTSEDQTQLS
                     RFATAMAQLLQRTQMLAYLQIELNQTQEKLESAKAKAKALQSENEDLHYQVQIANEQG
                     EKNLLRAESLAAIIANQGETGLTTPDQIAGVAPKGSTETEVSRLQSLLHAQTPDAHEP
                     TYMEGELRLALEEVARLKSALSEADQRHLAFINEQSDRLPINDKNDEIANIIQEMRQP
                     MSSVSGYTDFILSESIGILGALQRKFMERIKISTERMSQLVNELLRLTTVEMNLGKFT
                     ATGGADLYALVESAIAESMEAIRAKEINLRVDVAEKLPLLRADEHAIKQVLIKLLENA
                     KDVTPAKGEITLQASVQGGDHRQGYVLIQISDQGGGIPPEYVPLLFTRFSQANGKSIP
                     GTGGASSQLSIVKMLVENNGGRIWVDSQPNKGSTFSILLPVASENLFGDEPEDPLA"
BASE COUNT         2113 a         2139 c         2555 g         2116 t
ORIGIN      
        1 ttgagcttga gcaggcaagc cccactgcga aggagcccgg aatccaatgt tggagcgcag
       61 aaaactgcaa gcaaatggaa ttcgcaagat aacctgcttg acgtttccct ctttatcctt
      121 taagatacac ttcgcacaaa cattctaact ttacgtgcgg agtgcgaagt tatcacgtat
      181 cacattgcat gtgttatgtc tttcggcgcg tggcacatgg catttggtac gttaaatttg
      241 aaacgggaga acggattatg gaactagggc tggtccgcca aattgacatc gacgctgaga
      301 tgcagcaagc ctacctggat tatgccatga gcgtgattgt agcgcgcgcc ttgcccgatg
      361 cgcgcgatgg tctaaagccg gttcaccgcc ggattatgta tgccatgtat gatatgggac
      421 tgcgctcaga ctcagcgtat aaaaaatcag cccgcatcgt aggtgaagtg ttgggtaagt
      481 accacccgca tggcgatatg gcggtatatg aagccatggc gcgcatggcg caggattttt
      541 ccatgcgctg tctcctggtc gatgggcagg gtaactttgg ctctgtggat ggcgacccac
      601 cggctgccat gcgttacacc gaggcaagat tggcgtcgcc cgcccaacat atgctggcag
      661 atattcaaaa aaatacggtg gacttcgagc cgaatttcga cgataccctt aacgagccca
      721 cggtgttacc cgctgcgatt ccaaacctgc tggtgaacgg ggctacaggt atcgccgtag
      781 ggatgtccac cagcatccca ccccacaatt tgagtgaagt gattgatgcc ttgaagtacc
      841 ttctgggaaa ttgggagcgt ttggatgata tcgacctgga agacttgatg cgcttcatcc
      901 aagggcccga tttccccacc ggaggggtca ttatccagga gggcggcgag gagactctgg
      961 cgaccgccta tggctcgggg cgaggacgtg tgatggtgca agcgcgggcg catttggaag
     1021 agatggagcg cgggcgcaac cgaatcattg tcaccgaact gccgtatatg accaataaat
     1081 caacgctgat cgagcgcatc gctgaattag cgcgggatgg aaaactggaa gggctagccg
     1141 atctgcgcga tgaatctgat cggcaaggca tgcgcatcgt catcgaatta aacaaagccg
     1201 cagatccaga gacagtgcta cgtgacttgt acaagtacac ccccatgcag tctaccttca
     1261 gcatcatcat gctggcgctg gtagagggtg agccgcgcat gctaacctta aaacaggctt
     1321 tgcgcttcta cctggaacac cgcctggaag tcatccggcg taggagccag tatgatctag
     1381 ataaagcacg ccaacgggca catttgcttg agggcttgcg cattgccctg aagaatcttg
     1441 atgaaatcat tggcttgatc cgaaaagccc cagatgttga gacagcgcga gggcggttaa
     1501 ttagacgatt caagttaagc gatcttcaag cccaagcgat cctcgatatg cagttgcgcc
     1561 gtctggcagc actggaacgc aagaaaatcg aagaggaata caaagagatt ctcgcgcaga
     1621 tcaaagagtt ggaagggcta ttgcgttcgc cgaaaaagat gcgccagctt tgcgcagatg
     1681 agctgcaggc agtcaaagat gcatatggcg accgtcgccg cacccagatc gtacaattag
     1741 aagcgggaag agagaagcgc tctctattga ctattgaaaa ccttgcccca gagaagactt
     1801 cctggcttgt ggtgaccacc gatgggctgc tctctcgtac actggaggac aaagttcccc
     1861 gccaaagcgg caatgatgcg ccgaactggc tgctacgtgt gaatacccgt gatacgcttt
     1921 acctggtctg tgagcaaggg gagactgctg cagtacctgt ccttgctatc cctgaggcgg
     1981 ataaaccctc cgaaggcgtg atctacagca aggtttctgc cttgaaagac gaaaacaagc
     2041 tggcggtttt atttacctta ccaccaaaag gtgagcgcgc cgacgactgg tacgtgatca
     2101 ctgccacccg taagggtatg gtcaagaaat ctgcgattaa cgacctgcct ggcacatcgg
     2161 caaatatatt tactttggta aaagtcaatg aaggcgatcg tcttggatgg ttgcagctga
     2221 cgaatggggg caaggaagtg ctattggtga cagctttagg tatggccata cgatttaatg
     2281 aagacgaggt gcgaccgatg gggttggttg cggctggggt aatggggatg aagctccagt
     2341 caaaagatga agtagcaggt atggaaatcc tgccgcagcc aggcgaagtt tttattgcag
     2401 tctcggatgg cagcgccaag cgcgtcgcgc ctgctcaatt ccctaagcaa gggcggtatg
     2461 ggcaaggggt ggtcgcctgg aaactacctg gaaaagcgcg cgtgatcggc ttggcggtgg
     2521 gcaaaggtac ggcacgcgtc accctacact tgagcaagct ggctcctaag atgtcgcggt
     2581 tagatgaagc cccattgcag agcagggtgg cacgcggtgc caggatacag gagcttaagc
     2641 ctggagagca ggtgttacga ctgacagtgc cctgggatgt ggagcgcccc atagcaggag
     2701 ttaaagcaaa ggttggcaag cgggttcgac gatcgacggc agagtccaca tcgtccagat
     2761 ctgcaaagcc tgctccccgc aaaagaggcc cggccaaggc cagccaggct gaggctaaac
     2821 cagtcaagag cagatcgaag aaaccgtcta ctcgctcagg cagcgctaag ccagctcccg
     2881 ccaagcgaac ccaacggaga agcgggccaa aataaagctc agttgtgagg atctggtcta
     2941 ggtgattgag aaagaactga agtggtaagc gatcttgcct tcgatcagcc ccagagtatc
     3001 gctgccgttg ttcactttac ccgcgctcga ggtcgctgtc caggtgaagt ggcgtgagct
     3061 gccgctgccc gaaaaactaa ccagggtaaa gctggcattg ggcaggagtt gtgtaaacag
     3121 tgtttggtac cagttctgaa tggcgacggt accctggatg gtgcgagcgg cggtgacatg
     3181 cactgctgct ggggtataca agccgaccac ctgggcaggg ttgtgcgtat taagtgcggt
     3241 aatatattgt tctgtaatgt ccgtaggggg tggtgggggc ggctcggcgt cccatttata
     3301 atctcgaata gcattccaaa ccgatggcag gtaggtacgg caattggccc attcccagaa
     3361 gttggctgca ctcagattga gactttgcgc cgtgcgcaga aattccgtca cgtctgatgc
     3421 ggtggcagcc cagctacccg atttatatgc tgatccgacc gggataatgg gacgaaaggg
     3481 ggtcatggct tgaaattcgc tcaccgagcg acgtagctgg tcaccagggt tgtgggcgct
     3541 gacccagtac acctgcggca tattatatgt gcacttttcc agaaattcct tccagggaag
     3601 ctggggatga taggatggat agcggtatga gcacagtgca atggggaagt tgggtaacgc
     3661 cgagcgcagg cgactcatga attgtgctgc tgctttatcc ttcccaggtt gtttatactc
     3721 gccttcggca tcgatcacgt agccttgcat attggtctgc tggatgcgct ggatggcttt
     3781 attagcttca tcaatgggcg agtcgccata cacataatgc catccccaga cctggatgcc
     3841 gcgcgcccgc agcgcctggg ctacaggcgg gaccagatcc acaccgctgg aattaacgtt
     3901 ataactgtag gtgccatcgg cgatcttgat cagcacgtga ctcaagttag cctggtcggc
     3961 tagggaggca atcgccgagg cgttgccgcc ttccgtattg cgaatttgcc agataaagaa
     4021 ccctttcccc ttgagtggca tcttattctg cctgcccgtc catcgccttg cgcaacagtc
     4081 gcataatttt atccttttgg ggcagcaaca cctgcgctcc accggctgtt acgtaaggag
     4141 tgaccttgcc cgagccgaca aaatagttct tcacgcgttt gccatccccc agtttagcgg
     4201 caaggggcag ataaggcagc aaatcgaata ggttcaggtc cgtttttacg ctttcgtcgt
     4261 acaagtcgta tagttcaggg gcgcgttgga tagcatccag gctcaggaat ttgttgatca
     4321 atgcttgaag tatctcttgt tggcggcgat tacgagcaaa gtcgttgctg gtctttcggg
     4381 agcgtacata ccaaagaacc atatcggcgt tcatcgtccg cataccgggt ttgatggttg
     4441 tcagacgccc gcggtagtag tctgataatc tctctccaac ttggacatcc aaaccgccaa
     4501 gactatcgac aacccgcttg aagtttgaaa actcaatcaa gacgtaatgg tccacctgaa
     4561 cgccgaagtt atattcgaag gcggcggaaa gcttggagaa acctccccgc ccccaagcgg
     4621 tattgatgcg gtcctgtcca tggcccggga tagttatgta caggtcgcgc gggaacgaga
     4681 ccaggctaac cgttcctgct tcgggattca aactagccag tatgattgtg tcggtgcgaa
     4741 ataggctgcc tttgccccac caggagcgca tgtctgaacc aagcagaagg atattggtta
     4801 tgcctttggg ctcttcgatt gatccaactg gaccaaaatc ggtgggtagc gattcgaatg
     4861 tcagaacttc cggcgtggga attggggttt cggtggggat ttccgtgggc tcgaaagctg
     4921 ctgtgggcgg tatgggttgg aagggagtgg gtgtggccgt ggcatctggc ggtgcctcaa
     4981 gcagtggggg acgggcagtt tcggtggggg gcaataccgg cagtgaattg ggcatgccaa
     5041 ttgccccggg catgaagcag gcagacagcg tgctggctaa tagggcgagg atcaataggc
     5101 gtttcatagt ggacatagac ccagtctcat cattaaggat agggtatcgg aggttctgtg
     5161 cggatgggta cgaattccgt caccacaggg ggaggttctg ttcgaacagg ttgcgattgt
     5221 gtctggacgg gtacaggttc tgtagtatct gaaggcggtt ctgtggtatc agcaggtggc
     5281 tcggtaatct gcggtggcga ttctgtagtt aaaacaggtg gttcagtagc tacaggcaca
     5341 gaggtagctg ttcgttttgg tgtggcggtc ggcttgggag taataggagg atacccgggc
     5401 acgtaaatga tttgccccac tcggatcacg tctgatgtca ggcagttagc taccatcagt
     5461 tctctgctac tcagcccgta ggaaattgcg atgcggaata tggtatcgcc tcttttgact
     5521 ttgtaggcga cccaccttgc tggatgaccg ctgcagcgtg acggtgcgcg cgtggcttgc
     5581 ttgcttgttt tcttttgctc ggcagctgta ggacgcaggg tgctggtggc ggttggggta
     5641 gaggtttgtg ttggtggagg tactgaaatc agactcctag gtaaaagcgt gtctaaggtt
     5701 aggcagttgg cctgacgcag gatttctaca cttatgccat actccgctgc tatactatca
     5761 agcgtttccc caggcaacac ctcccgcgaa agccaaccag ctggtgtatc tttgcaagcc
     5821 gaggaaggct gtgcgggggt ttcggtgggg gtcggcgaca gggttggtgt gaaggtagcc
     5881 tcaccaggct ttggggtggc aacaagcggg gccgcagttt gggatggtgt gggtagcatt
     5941 gccaggtgta ccccaccttc tgttagggcg atgagtacac taccaaagac gagggctatg
     6001 gagaagaggg cggtcaatat tcctagggca acttgacgca agctgctcat gcaagcgggt
     6061 cctcgggctc atccccgaat aaattttcgg aagcaacagg caataggatg ctaaaggttg
     6121 agcccttatt aggttggcta tcaacccaaa tgcgcccacc attattctct acaagcatct
     6181 tgactatcga gagctggctg cttgctccgc ctgtacctgg aatagacttt ccattggctt
     6241 ggctaaaacg agtgaataat aacggcacat attctggcgg tatgccccca ccctggtcgc
     6301 tgatctggat aagcacatag ccttgacggt gatctcctcc ttgtacacta gcctgtaggg
     6361 tgatttcccc cttggctggg gtgacgtctt ttgcgttttc gagcaactta atcaagactt
     6421 gtttgatggc atgctcgtca gcgcggagta gaggtaactt ttcagcaaca tcgacgcgta
     6481 ggttgatttc ttttgcgcgg atcgcctcca tgctctcagc gatggcgctt tcaactaaag
     6541 cgtaaagatc agcccctccg gtggcggtaa atttacccag gttcatttca acagttgtca
     6601 atctcaacag ttcattaacc agttgggaca tgcgctcagt ggagatcttg atacgctcca
     6661 tgaatttacg ttgcagagcg cctaggatac caatcgattc acttaagatg aaatctgtat
     6721 aaccggatac tgaagacatt ggttgtcgca tttcttgtat gatgttagca atttcatcgt
     6781 ttttatcgtt gattggtaat ctgtcgcttt gttcatttat aaaggccaga tgcctttgat
     6841 ccgcttcgga gagagccgac ttcaatcgag ccacctcttc cagtgccagg cgtagctcgc
     6901 cttccatgta ggtcggttca tgggcatctg gtgtctgggc gtgcaagagg ctttgcagtc
     6961 ggctgacttc tgtctccgtg cttcccttgg gtgccactcc ggctatttga tcgggggtgg
     7021 ttagccctgt ctcaccctga ttggcgatga ttgctgccag ggactcggct cgaagaaggt
     7081 tcttctcacc ctgctcgtta gcgatttgga cttgataatg taaatcttcg ttttcgcttt
     7141 gaagtgcttt cgccttcgcc ttggcgcttt ccagtttctc ctgggtttgg tttagctcga
     7201 tttgtaaata ggcgagcatc tgagtacgtt gcaagagctg tgccatggcg gtggcgaaac
     7261 gagaaagttg tgtctggtct tcactcgtcc aagcccgatt ggagtatggt gatagcaata
     7321 aaagccctgc aattgggctg tcgtcggctg agacaaccgg cacaaacagc atgtgtcctg
     7381 gtcgctcaag atgcaagtgg cttgccagag ttcgcaaatc tgtggaagtg ctgctggccg
     7441 gcaggcgcag gggtaagcgg cgagtcaagg cggtggatat cagcgatact gattttgctg
     7501 aaagtgatga gcctgtcaga ttttcttcgc ggatcagatc aaaaccacat tggatattaa
     7561 tctcattctg ctcattgggt ggcgtaagca acagacaaat atcagctaca agggctgtgg
     7621 cgagggttcg ggtgatggcc tgacaaattc tatcgggttc actttcgcta cttaggtaca
     7681 gaaaactctc caagagcttg gggtcgcttg tggggcgttt gtgctccgct ggcacggcgg
     7741 gtattgggac ctgctgagta cttgttggga atcgctgagg caaagcgaac aagaaaggat
     7801 aatatgccat ctgggccatg cgcagcacgc cggaataatc accttcgggc gcaggcagtg
     7861 ccaggtaggc aacatgccca gcgagagcta tgcccagcat gccaagacca taaccccagc
     7921 tgtttggttt gcggacgagc aatatcacgc ctccaatgac aatcagggag atggcaacca
     7981 cttctccaat gaagtctggc caggagccgt tgtagtcaat accactccct tgccccaacc
     8041 accagaccaa gttgagcgcc aggccggtca ggccgagcag tccaagcagc caggtagcgg
     8101 cgtcagccaa gcggagtggt tcgggaatgc accacatcca gatgatgatg agtagactga
     8161 tcagggtcgc agcgcggtcc acaggaggaa gcaaaatgtg ctcgttgacc agaccttgcc
     8221 aggcgatgcc cgccaggaca aatatcacca agcgtatcgc cagcagcaag ctcagcccga
     8281 taatcagacg cctcccctgg ggaaactggt tgttgcgcca ttggctcaaa ctagcctgca
     8341 acgagccggc tacggaaaaa gacagcacca catggtaagt caggttgccg ggagattgtg
     8401 tcagcagggt gagtagttct tgtaggaaag tatccatagc catctcaacg attatagcat
     8461 gtcgcatagg catagaaaag gaggtaagac taacatttat gccaatcaaa atcaccagat
     8521 ttgtatcaca tctcgattga aagagggata aattgttacc aacttaggct taaggctttc
     8581 aacttttgaa aaccctgtca acttgaggcc agacaatttg agataagagg gcattatgga
     8641 gccgttttga ggttcattgg cgtaaaaatg cctcaacaag accgttttgc cgctaaatta
     8701 gccataaaat tacttgccaa agctactaaa atggtaaagt gtagctatga taaaccaatt
     8761 tcacccgcaa aatgactttc tgagtgtcaa aataaggtta aattccttat ctcaatttgt
     8821 ctgatttcaa ccggaaatgc tttacatcgc atatatcttt ctgtataatt taaaaaggtc
     8881 acaggcttag ttctacgaat attgggggag accatacatc ttt
//
LOCUS       DBNGSX010000600         2195 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1589580, whole genome shotgun sequence.
ACCESSION   DBNGSX010000600 DBNGSX010000000
VERSION     DBNGSX010000600.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1589580"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="AC16CB_17415"
     CDS             <1..938
                     /locus_tag="AC16CB_17415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344304.1"
                     /translation="KSYAFSENRLGGRQVLNHDGNGIGFGSRMILVPEHDLGIFISTN
                     HRQILFDANTPTPAFEFIRDLANAILEREVPETPQNISALTPLPDAAGRAGRYAGHYQ
                     LAGSPLKDFFKIQLLLDNVDVSDNGDGSITIGSKSYVEVEPLVFQRQDNASSFVVFIE
                     NARGEVEYLTFGGTASYKKAGWYQTLNFTIGLVAGMLLIFLSYIVTWPFLRQGSWLAW
                     AISLLNLIFLVGFGIMIATADIVLFFKMIPLPMRLLMALPWISALGTLGLLVSQFRRS
                     SRQGTSRWGQIHTSLMTIASLAFLWFVAYWNLLLR"
     gene            complement(1042..1461)
                     /locus_tag="AC16CB_17420"
     CDS             complement(1042..1461)
                     /locus_tag="AC16CB_17420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03667 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HIR3344305.1"
                     /translation="MIIDLTTEYGARVARRLQDEQVIWLTTVGRDGTPQPNPVWFIWE
                     NDSFLIYTQDTAYKLRNIRRNPRVSLHFDSGESGEDVVVFTGRASLDESAPLAHRHAA
                     YLEKYRQGIADIDMTPESMGKDYHVAIRVKPDKMRGF"
     gene            complement(1557..>2195)
                     /locus_tag="AC16CB_17425"
     CDS             complement(1557..>2195)
                     /locus_tag="AC16CB_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344306.1"
                     /translation="GAWLFINAIFGRRWHHIAAGFPPVTTFTVAMLLATALHWDRFDI
                     RHFPFILWLGLYVITPFLVPWMWLRNRVTDPGIPEANDLLVPVVARWSLRLLGVFLFI
                     FAVVGFIAPQWVIGLWPWQLTPLTARVLSGWFALLGVGGIVISRDPRWSAWRVGLQSI
                     GLWHILVVIGALFNQADFFNGLLNWYLISVTLVLIGMAVLYIQMESSRRSLA"
BASE COUNT          483 a          598 c          610 g          504 t
ORIGIN      
        1 gaaagtctta tgcattttcc gaaaaccgtc tcggtgggcg gcaggtgttg aatcatgatg
       61 gcaacgggat cgggtttggt agccggatga tcctggtacc ggagcacgat ttgggtatct
      121 ttatcagtac caatcatcgc caaattctat ttgatgccaa taccccaacg ccggctttcg
      181 aattcatccg ggatctggcc aacgcgattt tagagcggga ggttccggaa acgcctcaaa
      241 atatctccgc gctgacacca ttgccagacg cagccgggcg agcgggccgt tatgctgggc
      301 attaccagct ggcagggtcc ccgttgaaag atttcttcaa aatccaatta ctgctggaca
      361 atgtggatgt gagcgataat ggcgatgggt cgataaccat cggctcaaag agttatgtcg
      421 aagtggagcc gctggtcttc cagagacaag ataatgccag ttccttcgtc gttttcatcg
      481 aaaacgcccg aggtgaggtg gaatatctga cttttggtgg tactgcctcg tacaagaagg
      541 caggctggta ccagacgctg aacttcacca tcgggttggt ggctggcatg ctgctcatct
      601 ttctgtctta catcgtgact tggcctttcc tgcgtcaagg ctcctggctg gcctgggcaa
      661 taagcctgct caacctgatt tttctggtcg ggtttgggat catgatcgca accgcagata
      721 tcgtcctgtt cttcaaaatg atcccactgc ccatgcgctt attgatggcg ctgccctgga
      781 ttagtgccct gggcacgctt ggattgcttg taagccagtt taggcgctcg agtcggcaag
      841 gcacttcccg gtggggccag atccacacct cgctcatgac gatcgcctcc ctggccttcc
      901 tctggtttgt cgcttactgg aaccttcttc tgagatgagt gcaagaaatt agcacatgcc
      961 tcacgagtcg acttaatgtt cagccccctg cccacgcaat gaacgggtga aggggctgag
     1021 ccgcgcttgc tttgggatag cttaaaaccc gcgcatctta tctggcttaa ctcggatggc
     1081 aacatgataa tctttgccca tgctctccgg tgtcatgtcg atgtcggcga tgccttgccg
     1141 gtacttttcg agataagccg cgtgacggtg cgcaagggga gcgctctcgt ctagggatgc
     1201 cctcccggtg aagacaacta catcttcgcc gctctccccg gagtcaaaat gcagtgagac
     1261 gcgcggattg cggcggatat tgcgcagttt atatgcagtg tcttgggtgt agattaggaa
     1321 tgaatcattt tcccaaatga accatactgg gttgggctgc ggcgtgccgt cacgtcctac
     1381 cgtggttagc cagatgacct gctcatcttg caggcggcgc gccacgcgcg caccatactc
     1441 ggtggttagg tctatgatca tcagttctcc tttcactttc caacttgtcc caacaatcaa
     1501 cgggacaccg atgtagctta ctggctgttc ggggtgggtt aacctttgct ctggtgttaa
     1561 gcaaggctgc gccgactgct ttccatctgg atgtacagaa ccgccattcc gatcaagacc
     1621 aacgttacac tgatcaaata ccaattcaat agcccgttga aaaagtctgc ctggttgaat
     1681 agcgccccga tgacgaccag gatgtgccac aggccaatgc tttgtaatcc tacccgccag
     1741 gcactccagc ggggatcgcg cgatatgaca atgcccccca cacccagcag ggcaaaccag
     1801 ccgctcagca cgcgcgcggt cagcggggtg agctgccatg gccataagcc gatgacccac
     1861 tgcggggcga taaacccaac cacggcgaag atgaacagga aaacgcctag caatcgcagg
     1921 ctccagcgtg caaccactgg gaccagcaga tcgttggcct cgggaatgcc cggatcagtt
     1981 acgcggttgc gtagccacat ccaagggacg agaaatggcg tgatgacata caggcccagc
     2041 cacaggatga aaggaaaatg tctgatgtcg aagcggtccc aatgaagcgc agttgccagg
     2101 agcatggcga ccgtgaaggt tgtcaccggc gggaaccctg ccgcgatgtg gtgccagcgc
     2161 cggccgaaga tggcgttaat gaatagccat gcgcc
//
LOCUS       DBNGSX010000601         5522 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_759026, whole genome shotgun sequence.
ACCESSION   DBNGSX010000601 DBNGSX010000000
VERSION     DBNGSX010000601.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5522)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_759026"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="AC16CB_17430"
     CDS             complement(<1..295)
                     /locus_tag="AC16CB_17430"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR3344307.1"
                     /translation="MLVSRLLVAIILVPVALLAISYGGLPYNLLVVLILMLASWEYTN
                     LFRAGGLQPAGFLVLAGTLLLGLGRIWDGFGSASWMASLFILASMSFHLIAYER"
     gene            complement(285..1034)
                     /locus_tag="AC16CB_17435"
     CDS             complement(285..1034)
                     /locus_tag="AC16CB_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HIR3344308.1"
                     /translation="MMKEKNPVFLPEKIPTHIAIIMDGNGRWALSRGLPRLAGHRAGT
                     ENLRRVIEACIEFGIRYLTIYAFSTENWGRPQEEVQGLMRILEDVIDRELQELHDQGV
                     QLHHLGRLDRLHPSLRKKVLYAVDFTKHNSRLILNVAFNYGGRDEIVCAIQRMIEEGV
                     KADDVTDVMVSKHLFTAGVPDPDLIIRTSGELRGSNFLIWQGAYSEWYFTPTYWPDFD
                     KDALLQALHEFSSRDRRYGGVDLKDTAAHAR"
     gene            complement(1050..1607)
                     /gene="frr"
                     /locus_tag="AC16CB_17440"
     CDS             complement(1050..1607)
                     /gene="frr"
                     /locus_tag="AC16CB_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR3344309.1"
                     /translation="MLKDIYKEAEERMKGAIKALEEDLNGIRTGRASPVLIERLQVDY
                     YGNPTPLIQLASISVPEPRSLLIRPFDMSTLKAIERAILASDLGLTPNNDGKTIRLNL
                     PPLTEERRRDLVKVVHNRVEEARVAARNVRRDSIKDLREFEQAKMISEDERKNGEDEL
                     QKLTDRLIDEINHVGDQKEKEILEV"
     gene            complement(1719..2438)
                     /gene="pyrH"
                     /locus_tag="AC16CB_17445"
     CDS             complement(1719..2438)
                     /gene="pyrH"
                     /locus_tag="AC16CB_17445"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR3344310.1"
                     /translation="MENSLKYHRILLKLSGEALAGPQGFGIDPRRAEDIAKRVGEVRK
                     LGVDVAIVIGAGNVWRGRVGIERGMERATADYMGMLATVMNALALMDAMERIGLVTRV
                     QSAVEMHAVAEPYIRRRAIRHLEKGRVVILGGGTGNPFFSTDTAAALRAMEIGAEVLI
                     KATKVDGVYDSDPMINPDAVRFERLTYMDSINRRLDVMDSTAISLCMDNHLPILVLNL
                     WQTDVLRQAVFGEEVGTLITE"
     gene            complement(2497..3102)
                     /gene="tsf"
                     /locus_tag="AC16CB_17450"
     CDS             complement(2497..3102)
                     /gene="tsf"
                     /locus_tag="AC16CB_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIR3344311.1"
                     /translation="MAVSIEQIKALREATGAGIMDCRKALEASDGDYEKALEILREKG
                     IATAAKRADREASDGVVELYSHGNGRVGVMVEVNCETDFVARSEAFRTFAHELALQIA
                     AGAPQFIRQEDIPAEVLDLEVEKARQLAKEEGKPDAVIERIIEGKLEKYKNEVVLLRQ
                     LYIRDDSLTIEKLLMQNIGSIGENIIIRRFARWELGETTAN"
     gene            complement(3105..4088)
                     /gene="rpsB"
                     /locus_tag="AC16CB_17455"
     CDS             complement(3105..4088)
                     /gene="rpsB"
                     /locus_tag="AC16CB_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIR3344312.1"
                     /translation="MAVIAMKSLLESGVHFGHRTHKWHPAMKPYIFTERNGIHIIDLQ
                     KTVKALEQSYNLVRDTVAAGSSVLFVGTKRQAQETIKEDAIRAGMPYVTERWLGGMLT
                     NWRTIRQRVNELDRLERMRDKGDFERITKKEALLLSRKIERLDTLLGGIRNMNRLPEM
                     VFVVDVRREETAIHEANLLNIPVIALVDTNCDPRNVDYVIPSNDDAIRAIKLLVGRIA
                     DAAQEGLALRKEEVVEEMPAMAPMGAEEVELSDEELLGEATLAKLTSRTTFIAAEDED
                     VDIEIEEEEEVQEDGEEELEEEDWVEEEEDEVSGKHFEDEDEEEFEDEDEE"
     gene            4558..5301
                     /locus_tag="AC16CB_17460"
     CDS             4558..5301
                     /locus_tag="AC16CB_17460"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR3344313.1"
                     /translation="MRYFATVGSTNDLATRWVEASAPDLSLVVADEQTRGRGRLNRRW
                     FTPPGAALALSLILRPGVGEQDAFSPYFYTALGALATCDALPPQVRAQIKWPNDVIIT
                     QDLDPGKLAGVLAEAHWQGKELAAIILGIGINVAPSSVPPDEQLHYPATCVEAVVKAK
                     IDRWSLLHAVLERLLYWRERLSTQEFLAAWDARLAFRGEWVRVSFADQPPVEGQILGL
                     TAEGRLQLRDRQGVLHELTGGELNVRKGV"
     gene            5303..5506
                     /locus_tag="AC16CB_17465"
     CDS             5303..5506
                     /locus_tag="AC16CB_17465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344314.1"
                     /translation="MLDDFRQQADEITFEEEDYQPPARPKKYFLGMTPFQRFVIATML
                     LVITCVLSSFALLVTEKVVIPGF"
BASE COUNT         1165 a         1642 c         1446 g         1269 t
ORIGIN      
        1 cgcgctcgta ggcgatcaag tgaaaagaca tgctagccag gataaacagg ctggccatcc
       61 aggaggcgct cccgaaacca tcccatatcc gccctaagcc cagcagcagt gtccccgcca
      121 gaactaaaaa accagcgggc tggagcccgc cggctcgaaa caggttggtg tattcccaag
      181 aagccagcat caggatcaag accaccagca ggttgtaggg cagcccgcca tagctgatcg
      241 ccagcagcgc aacgggtacg aggatgatag ctaccagcag gcggctaacg agcatgcgcg
      301 gcggtatcct tcaggtcaac ccccccataa cgccggtcgc gtgacgagaa ctcatgcagc
      361 gcctgcagta atgcgtcctt atcgaagtca ggccagtaag tgggcgtgaa gtaccactca
      421 gaataagcgc cctgccagat taagaagttg ctgccgcgca gctctccaga tgtgcggatg
      481 atcagatcag gatcgggtac accagccgta aacaggtgct tgctcaccat gacatcggtc
      541 acatcgtcgg ctttcacccc ttcctcgatc atgcgctgga tggcgcacac aatctcgtcg
      601 cgcccgccat agttgaaagc gacattaagg atcaggcgtg aattatgctt ggtgaagtct
      661 acagcgtaga gcactttctt gcgcaaggaa gggtggaggc ggtcgagccg gccgaggtga
      721 tgcagttgca ccccctggtc atgcagctcc tgcagctcgc ggtcgatcac gtcctccaga
      781 atgcgcatca gtccctgcac ctcttcctgg ggacgacccc agttctcggt agaaaaggcg
      841 tagatggtca ggtagcggat accgaactca atgcaggcct caatcacccg gcgcaggttt
      901 tccgtgcccg cccggtgccc cgccaaccgt ggcaggccgc gcgacaatgc ccaacgcccg
      961 ttgccgtcca taataattgc gatgtgggtt gggattttct ctggcaggaa gacaggattt
     1021 ttttctttca tcatcacact gagattcggt cagacttcca gaatctcttt ctctttttga
     1081 tcaccaacat gattaatctc atcgatgaga cggtcggtaa gcttctgcag ctcatcttca
     1141 ccatttttac gctcatcttc cgagatcatt ttcgcttgct caaactcgcg caggtccttg
     1201 atactgtcac gccgcacgtt gcgagcagcc acacgggctt cttcaacccg gttgtgcacc
     1261 acctttacca aatcgcggcg gcgctcctcg gtgagcggcg gcaagttgag acggatggtc
     1321 ttaccatcat tattaggcgt caaccccagg tcggaagcca agatggctct ttcgatagcc
     1381 ttgagcgtgc tcatgtcgaa tggacggatt agcaaagagc gcggctctgg cacgcttata
     1441 ctggcaagct ggatcagggg cgtggggttg ccgtaataat cgacctgcag cctttcaatg
     1501 agcaccgggc tagcccgccc tgtccggatg ccgttcaggt cttcttctaa ggcttttatt
     1561 gctcccttca tgcgctcttc ggcttccttg taaatgtctt tcaacatgac ggcctcctac
     1621 ccctatagga tacccgaaaa tgtgaaggga gaaaaggggc aaatttctgg gttttcaata
     1681 ggtcaagacc cccttctggc gaagctctgg ggacaaattc actccgtaat cagggtgccc
     1741 acctcctcgc caaatacggc ctggcgcaac acatcggttt gccacaggtt tagcaccaga
     1801 atgggcaggt ggttgtccat gcacagcgag atagccgtgg aatccatcac atccaggcgg
     1861 cggttgatac tgtccatgta ggtgagccgc tcaaagcgca cagcatcagg attgatcatt
     1921 ggatcgctgt cgtagacacc atccaccttg gtggctttga tgagcacctc tgccccgatc
     1981 tccatggcgc gcagggcagc ggcagtatcg gtggagaaga agggatttcc cgtgccgccc
     2041 cccaggatca ctacgcgccc cttctccagg tggcggatgg cgcggcggcg aatgtacggc
     2101 tcagccacag cgtgcatctc taccgcagat tgcacccggg tcaccaatcc aatacgctcc
     2161 atcgcatcca tcagggctag ggcgttcatg actgtcgcca acatacccat atagtcggca
     2221 gtggcgcgtt ccatgccgcg ctcgatgccc acccggccgc gccaaacatt gcccgccccg
     2281 atcacaatgg ctacatccac accgagcttg cgtacctcgc ccacccgctt ggcgatatcc
     2341 tctgcccgcc ggggatcaat gccgaatcct tgcggaccgg ccaatgcttc accgctcaac
     2401 ttaagcagaa tgcggtgata tttgagagaa ttttccattg agttgctcca caattcagac
     2461 acaaaaagag ccaaccggtg gttggccctt caataattaa ttcgctgtcg tttcacccag
     2521 ctcccagcgc gcaaagcgcc ggatgataat attttcaccg atcgagccaa tgttttgcat
     2581 cagcagcttc tcgatggtca gagagtcgtc acgaatatag agctggcgca ataggacaac
     2641 ctcattttta tacttctcaa gcttcccttc gataatgcgc tcgatgaccg cgtccggctt
     2701 tccttcttcc ttggcaagct ggcgtgcttt ctctacctca aggtcgagca cctcggcggg
     2761 tatatcttcc tggcgaatga actgcggggc gcctgcagcg atctgcaggg ctaactcgtg
     2821 ggcaaaagta cggaaagctt ctgagcgagc gacgaagtcc gtctcgcagt tgacctccac
     2881 catcacgccc acgcgcccgt ttccgtggga atacagctcg accacaccgt ccgatgcttc
     2941 tcggtcggcg cgcttggcag cggtcgcaat gcccttctcc cgcaggattt ctagcgcttt
     3001 ctcatagtcg ccatccgacg cttctagagc cttacggcaa tccatgatgc ccgcgcctgt
     3061 ggcttcgcgc agcgctttga tttgctctat cgaaactgcc atgattattc ctcgtcctcg
     3121 tcctcgaatt cttcttcgtc ctcgtcttcg aaatgctttc cgcttacctc gtcctcttcc
     3181 tcttctaccc agtcttcctc ttccagctct tcctcgccat cctcttggac ttcttcttct
     3241 tcttcaatct cgatatcgac atcttcgtct tcggcagcaa taaaggtggt gcggctggtt
     3301 agcttggcaa gtgtggcctc acccaacagt tcttcgtccg agagctcaac ttcctctgca
     3361 cccatcgggg ccatggcggg catctcctcg accacttctt ctttgcgcag agctagaccc
     3421 tcctgagcgg cgtcggctat ccggcccacc agcagcttaa tggcgcggat ggcatcgtcg
     3481 ttggatggga tgacataatc cacattgcgc gggtcacagt tggtgtccac cagggcaatg
     3541 actgggatat ttagcaaatt ggcttcgtga atggcagttt cctcgcggcg cacatccact
     3601 acaaagacca tctctggcag gcgattcata ttgcggatgc cgcccaaaag cgtatccagt
     3661 cgctcgatct tgcggctcag caacaaagct tccttctttg tgatgcgctc aaaatcgcct
     3721 ttgtcacgca tgcgctccag gcgatccagc tcattaactc gctggcggat ggtccgccag
     3781 ttggtgagca taccgcccaa ccagcgctca gtcacatagg gcatgccagc acggatggcg
     3841 tcttccttga ttgtttcctg ggcctggcgt ttggtgccaa caaaaagcac cgaactccca
     3901 gcggcaaccg tgtcgcgcac cagattgtag gattgctcca gcgccttgac cgtcttctgc
     3961 aaatcaataa tgtggatgcc attgcgctcg gtaaaaatat agggtttcat cgcagggtgc
     4021 cacttgtggg tgcggtgacc gaagtgcacg ccgctttcga ggagcgattt catcgcgata
     4081 acagccattg ggtattctcc tttgcgttaa acctccgcct ctttcaaccc ttcccggtac
     4141 cgcgggcagg tagacaacct gcctaatcgg cggcacgccc gggaaagtcc ggagacgtgt
     4201 gagatgggat tttgtgccac aaacgccttc aggtgggcac aattgctccc gccacaaggc
     4261 gggagcagac cggagtatat catgtggtac aggattcgtc cagaggaaaa ttggcaatcg
     4321 gtttcggaaa ttagaaaaat ttatctccat tacattttta acaacccagg gcaattgcat
     4381 ataccctgag gggcataccc cccaccaaaa caccccattt tctctcctca gtggtgggaa
     4441 atcgcccaaa tgcgattgcc ctattaacaa cctaagttct cggtttttat gcggtaaaat
     4501 cgagatatag accagacctt tttacaatcc cttctcgccg atctacccct gggtgcagtg
     4561 cgttattttg ccacagtcgg ttccaccaat gatctggcaa cgcgctgggt tgaggctagt
     4621 gcaccagatc tctcgctggt ggtcgctgac gagcagaccc gcgggcgcgg gcgccttaat
     4681 cgtcgctggt ttaccccacc cggtgctgcc ctggctctta gcctgattct tcggcccggc
     4741 gtgggggagc aagatgcatt ctctccctac ttttataccg cgcttggggc gctggccacc
     4801 tgcgacgcct taccgcccca agttcgagcg cagatcaagt ggccgaacga tgtgatcatc
     4861 acccaagacc tagaccctgg taagctggca ggcgtcctgg ctgaagctca ctggcaaggc
     4921 aaggaactgg ctgccataat ccttggaatc ggcattaatg ttgcaccttc ttcggtaccc
     4981 ccggacgagc agctccatta ccctgcgacc tgcgtggaag ccgtagtgaa agcaaagatt
     5041 gatcgttgga gcttgctgca cgccgtgcta gagaggctgc tttactggcg cgagcggctc
     5101 agcacgcagg agttcctggc agcctgggac gcccggctgg cctttcgtgg tgaatgggtg
     5161 cgggtttcgt ttgcagatca gcccccggtg gaaggccaaa tactgggatt aaccgccgaa
     5221 gggcgcctgc aactgcgcga ccgccagggc gtgctgcacg agctgaccgg cggcgagctc
     5281 aatgttagga aaggagtttg agatgctaga tgactttcgc caacaggctg acgagataac
     5341 ttttgaggaa gaggattacc agccacccgc ccgcccgaag aagtatttcc tgggaatgac
     5401 accctttcag cgctttgtga ttgcgacgat gttgcttgtg attacctgcg tgctgtcatc
     5461 cttcgcgctg ctggtgacag agaaggtagt gattccagga ttttgagaca aacacaatcc
     5521 tt
//
LOCUS       DBNGSX010000602         3820 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_620477, whole genome shotgun sequence.
ACCESSION   DBNGSX010000602 DBNGSX010000000
VERSION     DBNGSX010000602.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_620477"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..1300)
                     /locus_tag="AC16CB_17470"
     CDS             complement(233..1300)
                     /locus_tag="AC16CB_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR3344315.1"
                     /translation="MKKLPVLLTIIMVLSLLLTACGGQATPTEEAAPEVATSEPAETE
                     GAAPAAEDKWCSGTNIIFFPGGAPGGPFETVVYNGAVAAAADLGPNVEYVWSDWNPEK
                     MITQFSEAMATNPDGIAVMGHPGDEAFGPLVDEAEAKGIVVTSMNTQLAALQAKYSSS
                     GFGYVGAILYDAGWALGKESITRAGLQSGDKAFVWGLLAQAGRGERTKGIVEALEDSG
                     IEVIYQEIDDATNADAAAGIPVFVGIMSSNPDIKLVVTDHGNLTGTQQSFFESAGLGP
                     DDVFGSGFDLSAATVEAIRGGWTDLVIDQQQWLQGYMAILQICITHNYAFTGLHIDTG
                     AGFAHKDNVELLAGLVDKQIR"
     gene            complement(1586..2227)
                     /locus_tag="AC16CB_17475"
     CDS             complement(1586..2227)
                     /locus_tag="AC16CB_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HIR3344316.1"
                     /translation="MLEQLKQALWEYHMELPRNNLVVWTSGNVSALDRESGYVVIKAS
                     GVRYEALRPEHMIVVDMDGKVIEGNLKPSSDTASHLYIYRHRSDVGGIVHTHSRYATA
                     FAAVGKTIPVYLTAHADEFGCEIPCAGFALIGGEEIGMQVVENIGKSTAVLLKNHGVF
                     TIGKDAESAVKSAVMVEDIAATVWIALQLGTPDRISSEDVEKLHHRYTHVYGQ"
     gene            complement(2235..>3820)
                     /gene="araB"
                     /locus_tag="AC16CB_17480"
     CDS             complement(2235..>3820)
                     /gene="araB"
                     /locus_tag="AC16CB_17480"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HIR3344317.1"
                     /translation="TAVYPYANGVIDERLPESGVLLEPDWALQDPEDYIRVFQNAIPK
                     VLQQSGVDAADVIGVGIDFTACTMLPVKADGTPLCTLPEWRATPHAWVKLWKHHAAQP
                     EADHINEAAREMNQAWLDRYGGKISSEWFFSKALQILNDAPDVYAAADRLIEAADWVI
                     WQLTGVETRNSCTAGYKAIWSKRDGFPANEYFASLHPQLTNVIEDKMSRNIQPIGQKA
                     GGLSEKAAAWTGLKPGTAVAIANVDAHVAVPAATVTEPGRMVMIMGTSICHMVLGTEE
                     HMVPGMCGYVEDGIIPGYFGYEAGQSCVGDHFAWFVENCVPASYEREAKERGVDIHAL
                     LEEKAAQLKPGESGLLALDWWNGNRSVLVDVDLTGLLIGATLSTKPEEIYRALIEATA
                     FGTRVIVETFQQNGVPIYELVACGGLPEKNKLLMQIYADVTGRPFKVSSSQQTSALGS
                     AMFGAVAAGSAAGGYDSIYEAAQKMAKLKDEVFIPILEHQKVYDQLYAEYLRLHDYFG
                     RGENTVMKKLKRIKVEARM"
BASE COUNT          904 a         1063 c          904 g          949 t
ORIGIN      
        1 attctggcct aattgaaaat cgatattctc aagggcctgc acttccccaa agtttttgga
       61 tacattcctg agctctaggc gatattgctc catattcctc tccaaaagtg ctcaatcacg
      121 aatacataaa ttgtaacatt catcaaggtg acagtcacct cggacgtgac tgtcacctga
      181 ttgctcatcc ggtcagatag accaaaattg gaatacggaa ccggatttaa ccttagcgaa
      241 tttgtttatc gaccaaccca gccaacagct caacattgtc tttatgggca aagcctgcgc
      301 cggtgtcgat atgcagcccg gtgaacgcgt agttgtgggt gatgcagatc tgcaagattg
      361 ccatatatcc ctggagccat tgttgctgat cgatcaccag gtctgtccag ccgccgcgga
      421 tagcttctac agtggcggcg gagaggtcga acccagatcc gaagacatca tccgggccca
      481 agccagcact ttcaaagaag gactgctgtg ttccggtcaa gttaccgtgg tccgtgacaa
      541 ccagtttaat atccgggttg gaggacatga ttcccacgaa taccggtatg ccagcagcag
      601 catccgcgtt ggtggcatcg tcgatctcct gatagattac ctctatacca gaatcttcga
      661 gagcctcgac aatccccttg gtgcgctcgc cgcgtcccgc ctgcgccagc aacccccaga
      721 cgaaagcctt atcaccggat tgcaatccag cgcgagttat tgactctttg cctaaggccc
      781 aaccggcatc gtaaaggatc gcgccgacat agccaaagcc actagaggaa tactttgctt
      841 gcaaggcggc aagctgagta ttcatgctgg tcacgacaat gcccttggcc tcagcctcat
      901 ccacaagagg gccgaaagct tcatcgccag ggtgacccat gaccgcaatc ccatcgggat
      961 tcgtagccat ggcctccgag aattgggtga tcatcttttc gggattccaa tcggaccaca
     1021 catattccac gtttggacct aggtcagccg cggcggcaac cgcgccatta tagaccacag
     1081 tctcaaaggg accaccggga gcaccacccg ggaaaaagat aatgttggtg cccgagcacc
     1141 atttgtcctc agcagcgggt gcagcgcctt cggtctcagc gggttcgctt gtcgcgacct
     1201 caggtgcagc ttcttcggtg ggggtcgctt gtccgccaca ggcagtcagc aacaagctga
     1261 ggaccataat aatagtgagc aacactggaa gctttttcat cttttcatct ccttatactc
     1321 atctggtatt gatagaatgg aaacaaaatg cggggagtct tttacttacc catggacacc
     1381 tcctcttgca ggtgtggtct ggcatcaagt ggatcattaa gataggtgca tgattggtca
     1441 aattcatgtg atcttgttat cgttcccgtt atcgttcaca aaatcgtaac tatattatcg
     1501 ataaaaaaag ggggggtgtc aagaggttgc gaatttgtgc tgatccatat caaacccggt
     1561 tcatgtatga tcgtgcgata gaaatttact gcccatatac gtgggtatag cggtggtgta
     1621 gtttctccac atcttcagat gagatcctat ctggtgtgcc aagctggagc gctatccaga
     1681 cggtggcagc aatatcctcg accatcactg ccgacttgac ggcagattct gcatcctttc
     1741 caattgtgaa gacgccatgg ttctttaata gaacagccgt cgattttcca atattttcga
     1801 ctacttgcat gccaatctct tcacctccaa tcaacgcgaa tccagcacaa gggatttcac
     1861 agccaaattc atctgcgtgt gctgttagat aaacagggat agttttgcct accgctgcaa
     1921 atgcggtggc atatcgcgag tgtgtgtgta caataccgcc aacatcagag cgatgacgat
     1981 agatatacaa atggcttgcc gtatcagaag atggctttag gttcccttcg atcactttcc
     2041 catccatgtc aaccacgatc atatgctcgg ggcgcaatgc ttcatatcgt accccagatg
     2101 ccttgatcac cacataacct gattctcggt ccagtgcact gacattaccg ctcgtccaga
     2161 ccaccagatt gttcctgggc aattccatat ggtattccca aagagcttgt ttcagttgtt
     2221 ctaacataat tcactcacat tcttgcttca accttgatac gcttaagctt cttcatcaca
     2281 gtattttcac cacgcccaaa atagtcatgc aaacgtaaat actctgcata caactggtca
     2341 tagactttct ggtgttcaag aatgggtata aacacttcgt cttttagctt ggccatcttt
     2401 tgcgcggcct catagatcga gtcgtaacca cccgccgcgc tacctgccgc taccgcgccg
     2461 aacatggcgg aacctagagc agatgtttgt tgagatgaac taaccttgaa tggtcgtccc
     2521 gtcacatcgg cgtaaatttg catcaggagt ttgttctttt ctggcaaacc accacatgcc
     2581 accaactcgt agatgggtac tccattttgc tggaatgtct ccacaattac gcgtgtgcca
     2641 aaggcagtgg cttcgatcag agcccggtag atctcttcag gttttgtgct cagggtagca
     2701 ccgatcagga gaccggtcag gtccacatcc accagcacag agcgattgcc gttccaccaa
     2761 tccagcgcca gcaggccgct ttcccctggc ttgagttggg cggctttctc ttcgagcaag
     2821 gcgtggatat ccacaccgcg ttcttttgcc tcgcgctcat aggatgccgg gacgcagttc
     2881 tccacaaacc aggcaaaatg gtcaccgaca caggattgcc cggcttcata gccaaaatac
     2941 ccgggaataa tgccgtcttc cacgtatccg cacatgccgg gcaccatgtg ttcctctgta
     3001 cccagcacca tgtggcagat ggaggtcccc atgatcatga ccatgcgtcc cggttcggtc
     3061 actgtcgccg ccggaacggc cacatgtgca tctacgttgg cgatggctac agccgtgccg
     3121 ggtttcagtc ctgtccatgc cgccgccttc tcgctcagtc cgccagcctt ctgaccgatg
     3181 ggttggatat tgcgggacat tttgtcctca ataacgtttg taagttgcgg atggagtgag
     3241 gcgaaatact catttgcggg gaaaccatcc cgcttagacc atattgcttt ataaccagcc
     3301 gtgcaagagt tcctcgtctc cacccctgtc agctgccaaa tcacccaatc ggcagcttcg
     3361 atcaagcgat cagcggcggc ataaacatct ggcgcatcgt tcaagatttg caaggctttc
     3421 gaaaaaaacc actcggatga aatcttgccg ccatagcgat caagccaggc ttgattcatc
     3481 tctcgagctg cttcatttat atgatccgct tcgggttgag cggcgtggtg cttccacagt
     3541 ttcacccaag cgtgcggcgt agcgcgccat tcaggcaatg tgcacagcgg agttccatct
     3601 gccttcaccg ggagcatagt gcaggcggtg aaatcgatgc ccaccccgat cacgtcggca
     3661 gcatctacac ctgattgctg tagaaccttc gggatggcat tttgaaacac acggatataa
     3721 tcttcagggt cttgcagcgc ccagtccggc tcaagcaaaa caccgctttc cggcagcctt
     3781 tcatcgatga cgccgttcgc atacggatat accgccgtcg
//
LOCUS       DBNGSX010000603         2992 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_135829, whole genome shotgun sequence.
ACCESSION   DBNGSX010000603 DBNGSX010000000
VERSION     DBNGSX010000603.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_135829"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="AC16CB_17485"
     CDS             <1..208
                     /locus_tag="AC16CB_17485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344318.1"
                     /translation="KDAQISALQAENLSQGQQLADLDARITALERHSFKSGAQIYWYL
                     AMGAALLLGVMLGRGERFYSKVRR"
     gene            395..2980
                     /locus_tag="AC16CB_17490"
     CDS             395..2980
                     /locus_tag="AC16CB_17490"
                     /inference="COORDINATES: protein motif:HMM:NF016906.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HIR3344319.1"
                     /translation="MKKYTARFLFAAIFAAMMMGGCGKGPPPTATPSAPTSIPPTTKP
                     TTPPPTPAATSVPQGKTILVTSAADSGPGSLRQALQDAEQHDTITFDTNVFPPGSPVT
                     IYLTSGLPELTQGNLTIDASDSGVILDGSKMTTPDPIHGLSISSNNNTIRGLQLVSFS
                     DAGIALHSGAQHNIIGGERSTGAGPLGQGNLLVGNGNFGIGLWGEGTSFNVIQGNYIG
                     IDLEATATWGHPRDGIHSNGANDNLITGNLIGGNDTGVYLCCVAEGRNRVTDNIIGTD
                     ASGTISLGNRQAGVLIDRTGNNMIGPGNIIAHNDGAGIMFWGDTPRNTITQNSIYDNG
                     GQGIGSDFSSNVRLDAPLILDFDLQAGSLAGLACSNCTIEIFSDNSDEGAIYEGQATA
                     DSSGVFAFSKGSSFTGPHLTAAVSDPEGNTSQFSLPTSGASLSVMLQKENDLPRATIV
                     RGGFTQLADNRIGDMFPLDRHSPPCPPAEEDWSFTHLSLLGLKWARLSLDRLELDQAR
                     DMGDYSQFEINACQDEMVSLLAENDISILYTIVYWDEGLHAESYPDYRNEDEIQRFLD
                     YTRLIVRHFKDRIQYYEILNEALVYVEPIDYINLIRRVAPIIRDEYPQAKIVVGGSSN
                     LLYKECRDYLFAVLRSDIMPLVDGVALHPMYGASPQYDDTRQYYEGYPSLVQEIKDVA
                     SDHGFAGEFIAEEMAWRTTLNPNPYEPWEYTPTVAAKYYARGIMMHLGMDLWAGIGGE
                     NYDQIPPIVKVVQNLSGSMAGAKPAEIAVEIQSEAANIMSFGFSLPDGEMLLALWTNG
                     AAIEDDPGVSATLTFPGLSAQKVVGIDVLNGFEQEMLIDNQDDNLLINNLLVKDYPIL
                     LRLIP"
BASE COUNT          734 a          770 c          776 g          712 t
ORIGIN      
        1 gaaggacgca caaatcagcg ctttgcaagc cgagaatctc agccagggtc aacaactggc
       61 ggatttggat gcgcgtatca cagccttgga aaggcattct tttaaatctg gggcgcaaat
      121 ctattggtat cttgccatgg gcgccgccct tctacttggt gtgatgttag ggcgaggtga
      181 gcgattttat tcaaaagtaa gacggtgaaa tacaagacga tttgcgtgga ccaggctgga
      241 tgcgaatggc gctgtgttga gcagcgccag cagagaaagt gttcattgca aaatacatag
      301 gcgcgttttt acaatattat gtaataataa attgcaataa cgcttgtttt tccgctaatg
      361 ttgactgttg aaacacttga agttgaggtt agcgatgaaa aaatataccg cgcggttcct
      421 gtttgctgct attttcgccg ctatgatgat gggtggctgc ggaaagggtc ctccccccac
      481 tgctacaccc tcagccccta catcgattcc ccccacaaca aagcccacga cacctccgcc
      541 cacacctgcg gcgacgtccg tcccgcaagg aaagactatc cttgtcacta gtgccgcaga
      601 tagtggaccg ggcagcctgc gccaggcttt gcaggatgct gagcagcacg acacgatcac
      661 attcgatact aatgtcttcc cgccaggcag cccggtaacc atctacctta ccagtggctt
      721 gccagaactc actcaaggga acctgacaat agacgccagc gactccgggg tgatcttgga
      781 tggaagcaag atgaccaccc cagaccccat acacggcctt tcgatctcat ccaataataa
      841 taccatccgc ggtttgcaac tcgtaagctt ttccgatgcg gggattgcct tgcatagcgg
      901 cgcacagcac aatatcatcg gcggcgaaag gagcaccggt gcaggccctt tgggtcaagg
      961 caacctgctt gttggcaatg gcaactttgg tatcgggctt tggggcgaag gcacttcttt
     1021 taatgtcatt cagggcaact atattggcat cgacctggag gctacagcga catgggggca
     1081 tccacgcgat ggaatccact cgaacggtgc caacgataac ctgatcacag gcaacctgat
     1141 cggcggcaac gataccgggg tgtacttatg ctgtgtggcc gaaggaagga atagggtcac
     1201 agacaacatt attggtacgg atgccagcgg aacgatttcg ctgggcaacc gccaggctgg
     1261 cgtgcttatc gaccgcaccg gcaacaacat gatcggcccg ggcaatatta tcgctcacaa
     1321 tgatggagcg ggaatcatgt tctggggtga tacccctcgc aataccatca cccagaacag
     1381 catttacgat aacggtgggc aaggcattgg ctcagacttt agctctaatg tcaggttgga
     1441 tgctccgcta atcctcgatt ttgacttgca ggctggcagc ctggccggct tggcctgctc
     1501 caattgcaca atcgagattt tctctgataa tagcgatgaa ggtgccatct acgagggaca
     1561 ggcgacggct gacagttctg gagtttttgc tttttcaaaa gggtcttcat ttacaggccc
     1621 tcatctgacc gccgcggtct ctgaccctga aggcaacaca agccagtttt cattgcccac
     1681 cagcggcgcg tctctatctg tgatgcttca aaaggaaaac gatctgccga gagccacaat
     1741 cgtccgcggt ggatttacgc agctggcaga taaccgtata ggggacatgt tcccgctaga
     1801 caggcattca cccccctgtc ctccggcgga agaagattgg tctttcactc acctgagtct
     1861 gctgggtttg aagtgggcta ggctgtctct cgaccgctta gaattggacc aggccaggga
     1921 catgggggat tattctcaat tcgaaatcaa cgcctgccag gatgagatgg tttccttact
     1981 agcagaaaat gatatttcca ttttatatac catcgtatat tgggatgagg gactccacgc
     2041 ggagagctac ccggattatc ggaacgagga tgagatacag cgctttctcg attacactcg
     2101 attgatcgtg cgccacttca aggatcgtat tcaatactat gaaatattga atgaggcgct
     2161 tgtttatgtg gaacctattg actacatcaa cctcatccgc cgtgttgctc caatcatccg
     2221 ggatgaatac ccacaggcga aaatcgtggt gggtggctcg tccaatttgc tctataaaga
     2281 gtgccgcgat tacctgtttg ctgtactcag atccgacatc atgcccctgg tggatggcgt
     2341 cgccctgcac cccatgtacg gagcctcacc ccaatatgat gacacccgac aatattatga
     2401 aggctatccc tctctggtgc aagagataaa ggatgtggct tccgatcacg gattcgctgg
     2461 agagttcatt gccgaagaga tggcttggag gacgactctc aatcccaatc cctatgagcc
     2521 ttgggaatat actccaactg tagctgccaa gtactatgct cgaggcatca tgatgcacct
     2581 gggaatggat ctttgggcag ggattggtgg ggagaattat gaccaaatcc cgcctatcgt
     2641 gaaggtagtc caaaatctta gtggcagcat ggctggagcc aaacctgcag aaatagccgt
     2701 cgaaatccaa agtgaggcag ccaacatcat gagctttggt ttctcactgc ccgatggtga
     2761 gatgctgtta gccctctgga ccaatggcgc ggcaatcgaa gacgaccctg gcgtgagcgc
     2821 aaccctcact ttccctgggc tgtctgctca aaaagtggtt gggatagacg tgctaaatgg
     2881 cttcgagcag gagatgctca tagacaacca ggacgacaac ctgctcatta ataatttgtt
     2941 ggttaaggac tatcctatcc tccttcgctt gatcccctag attctgtttg ca
//
LOCUS       DBNGSX010000604         7286 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_898728, whole genome shotgun sequence.
ACCESSION   DBNGSX010000604 DBNGSX010000000
VERSION     DBNGSX010000604.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7286)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7286)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7286
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_898728"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="AC16CB_17495"
     CDS             complement(<1..389)
                     /locus_tag="AC16CB_17495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3344320.1"
                     /translation="MSKQSPLLYSQLADWFHLLTRPEDYAEEAEFYRRTILSTSPLPP
                     STLLELGSGGGNNASHMKAHFQLTLVDISPHMLSISRSINPECKHILGDMRTIRLSRV
                     YDAVFIHDAISYLTTLSDLEQAIQTAW"
     gene            complement(425..1348)
                     /locus_tag="AC16CB_17500"
     CDS             complement(425..1348)
                     /locus_tag="AC16CB_17500"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3344321.1"
                     /translation="MLRRSDPRWAYVLVNLATLLWASNIVLGRFLRHEIGPLSLSAAR
                     FSVAGLIFLLLLRKTQKNQALIDQPLGSNPTQPPRMEWLLYLGMSLVGVFGFTTLLYF
                     ALRYTTASHAALINAATPLVTAVLAAVFLKEQFTRPLIVGSILSLAGVGLVIGVGTTN
                     IAGSPQALFGDLLCLVAAVLWGIYSILGRLATKTKSSLQVTAFSIWFALPLLYLAAGV
                     EWQTTPPSLTSPVLLAAVYIGVFPSVIAFSAWNEGVRMAGPNQAMAFYNMLPVYGTLL
                     GVLLLGESLSWNALFGGILVIAGGIMIARLR"
     gene            complement(1371..2441)
                     /locus_tag="AC16CB_17505"
     CDS             complement(1371..2441)
                     /locus_tag="AC16CB_17505"
                     /inference="COORDINATES: protein motif:HMM:NF024891.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransacetylase family protein"
                     /protein_id="HIR3344322.1"
                     /translation="MKSLYITSVERYSGKTATCLALGKKLQSDGYKVGYLKPFSLQPW
                     RVSGHVADEDAAFVIEALGLEAQPWELSPVVVTSEALRQRLHQPQPEDLLDRVKAVAQ
                     SIGANVDMLLVEGGASLREGYIFDLSTPRVAEALGSPVLVLIKYQDPVRLMDDALTAT
                     FRLGKLLVGAIINRVPDEARQFVTQDAVPALERLGVPVFGVLPERRGLEALTVEELIE
                     VLNPQVLAAGSDPQALVETLTVGAMTADAALSRFRRQMNKAVITGGDRTDIQLAALET
                     STTCLILTGNLQPSPLIVKQAEEFGVAVLLVPTNTMETIEAIEKVFGKTHLAQPSKLE
                     HFQELVAANVDMARLYKAIGLT"
     gene            complement(2488..4653)
                     /locus_tag="AC16CB_17510"
     CDS             complement(2488..4653)
                     /locus_tag="AC16CB_17510"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012901660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043758 - acetate-CoA ligase (ADP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HIR3344323.1"
                     /translation="MLKPFFQPDSVAVIGASSNPSKLGHAVLKNLVDGGYAQRGMVYP
                     INPGANSILGHKAYPSVLDVPAAIDLVVIVIPYPQVPDALRECGQKGIPAAIVISAGF
                     REAGMEGFERERELIQIAKEYNLRLIGPNCLGVIDTFTPLNASFSAGTPPSGPMAFMS
                     QSGALGTAVLDIALAGRLGLSKFVSLGNKADVSEIDLLQAWKDDEHTRVILIYTEGMP
                     NGEEFIRVARQITPKKPVIAIKSGVTQSGSRAVSSHTGSLAGSEQAYQAAFNQAGILR
                     AESMQALFDMALAIGYQPELRGNRIAIITNAGGPGILATDALERAGLSMARFEIETIH
                     ALEQYLPDAASAANPVDVLGDARADRYQFAMEKVIADPNTDGLMIVLTPQAMTDIEAT
                     AEAIGALADATDKPILACFMGEARVEKGIEILNRFGVPNYPFPERAADAFRAMAKYQE
                     IKSRQTSQTQPIEADIKLVQSVINRVRSEKRTTIGDSEARDILQAYGLHIPRSEIASS
                     PERAIEIASQIGYPVVLKIASPDILHKTDVGGVKVGLQNAEAVRDAFELMVYRAQRYI
                     PEARIWGCLVQEMAPAGGLEVLVGMNRDPQFGPLVTFGLGGIYVEILKDVTFRIAPFS
                     REEAEAMIGEIRAHALLDGVRGKPPMDKEAILDTIMRIGQLVQDFPEIAELDINPLMV
                     YPHGQGAIAIDMRLVLQDSHKEASQTPTSPLPTPLSKEA"
     gene            complement(4664..5326)
                     /locus_tag="AC16CB_17515"
     CDS             complement(4664..5326)
                     /locus_tag="AC16CB_17515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344324.1"
                     /translation="MTFQKIRFALVIVTLLIATLACQLVSEIGETKATVESVATEAKE
                     GLNIIGTARALVTEVGGSEMIQTAQALATEVGESGVLQTAIAVATQEGPSVIETAKAV
                     ATQEGPGLQETAKAAMTQIAGSMDEAPPDIPIVNGETDNFLGNEFMVSYMTPLSLPEV
                     VEFYKNQMPVMGWTFVDKGTVETENMAALNYEKPDRAAAITLQVNPLDERTIVLILIS
                     EK"
     gene            complement(5516..>7286)
                     /locus_tag="AC16CB_17520"
     CDS             complement(5516..>7286)
                     /locus_tag="AC16CB_17520"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF025038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HIR3344325.1"
                     /translation="LRPLMENLQNKYQGVESVLTHLEAIQKDIVEHSDQFRRSEGSPS
                     SSNSSSQQAKQDWLGRYDVNVLVDNSDLKGAPVILESYPSYTNLLGRIEHEVIMGASR
                     TDFSMIRSGALHRANGGYLVIPARDILINPYAWEGLKRVLRDSEIRIVELGQQLGLLS
                     TVTLEPEPFPLNVKIVLVGTPVLYYLLRMYDEDFAKLFKVRAEFATTMKRTLETEQEY
                     GLFVKSVLDDNQLSPFDRSAVARIIEHSARMAEDQNRLTTRFGKIADLIREAAYWAEQ
                     ADKAADEQAGMPKKRKSKSAKSAQKSIIVSGEAVKKAIEESIYRSNLIEERIQELFAI
                     GTLMIDLTGSVIGQINALSVLPLGDYAFGRPSRVTAVAYAGKGGVVDIERLAKLGGPV
                     HTKGVLILSGLLGSRYGRKQTLGLSASLTFEQSYDEIEGDSASAAELFALLSAIAGIP
                     LRQDRAMTGSINQRGQIQAIGGVNEKIEGFYLTCKTKGLTGEQGVIIPLSNVGNLMLR
                     DEVVQAVEDGQFHVWPIHTIEEGIHLLTEMEAGELQPDGTYPAGTFNHAITQKLGEFA
                     KLAETLKDSNTKGNTANRAPGSS"
BASE COUNT         1625 a         1922 c         1982 g         1757 t
ORIGIN      
        1 atccatgcag tttggatagc ctgttcgagg tcgctgagcg tggtgagata actgatcgcg
       61 tcatggatga agaccgcatc gtagacgcgg ctgagcctga ttgtccgcat gtcacccagg
      121 atatgcttac attcggggtt aatcgagcgg cttatagaga gcatgtgtgg agagatatcc
      181 accaaagtga gctggaagtg cgccttcatg tgggaggcat tattgccgcc cccgcttccg
      241 agctcaagaa gtgtgctcgg cggcagaggt gaggtagaca ggatggtgcg ccggtagaat
      301 tcagcttcct cggcgtaatc ctctgggcgg gtgagcagat gaaaccaatc cgcgagctgg
      361 ctgtataaga gtggggattg cttcgacaat tagcagcttg ctcctagtgc aggtgtggaa
      421 gatgctagcg caatcttgcg atcatgatgc ccccggcaat gaccagtatg ccgccgaaca
      481 gggcattcca actcagagac tctcccagga gcaatacccc gagcagcgtg ccatatacgg
      541 ggagcatgtt ataaaacgcc atggcttgat ttggccctgc catgcgcacc ccctcgttcc
      601 aggcggaaaa agcgatcaca gatggaaaga cgccgatata cacggctgcg agtagtacag
      661 gagaggtcag tgaaggaggt gttgtttgcc attcaactcc ggcagccaga tagagcaaag
      721 gcaaggcgaa ccagatggaa aatgctgtaa cctgcaggct ggatttggtt tttgtcgcca
      781 gccggcctaa gattgaatag ataccccaga gtacagctgc caccagacag agcaagtcac
      841 caaacaaggc ttgtggagat ccagcgatat tggttgtccc aactccaatg actaaaccta
      901 cgccagccag ggataagatg ctcccgacga tgaggggacg ggtaaattgc tctttcaaaa
      961 atactgctgc caatactgct gtaaccaatg gggtagctgc attgatcagt gctgcgtggc
     1021 tggcggtggt atagcgcaag gcgaagtaaa gcaaagtcgt aaaaccgaat acaccaacta
     1081 aactcatccc aaggtatagg agccattcca tccggggtgg ctgggttgga tttgagccca
     1141 gcggctggtc aataagagcc tgattttttt gtgtctttct tagcaggagg agaaaaatca
     1201 acccggcgac actgaagcgt gccgcggaga gagagagggg gccaatctca tgacggagaa
     1261 accgtccgag aacaatattg cttgcccaga gtagcgtggc aaggtttacc agaacatatg
     1321 cccagcgagg gtcactgcga cgcaacattg ttcgagagga aattaattct ctaggtcaag
     1381 ccaattgctt tataaagcct agccatgtct acattggctg ccaccagctc ctggaaatgc
     1441 tccagcttgg atggctgagc taggtgagtt ttaccaaata ctttctcaat cgcctcgatt
     1501 gtctccatag tgttggtggg taccagtaaa accgctacgc cgaactcttc ggcttgcttg
     1561 acgattaacg ggctgggttg caggttgccg gtcaatatca gacacgtggt ggatgtctcc
     1621 agggcggcca gttggatgtc ggtacgatca ccgccagtga tgaccgcttt attcatttgt
     1681 ctgcggaagc ggctcaaggc ggcatcagcg gtcattgccc ccacggttaa tgtctcgacc
     1741 aatgcttgag gatcagatcc agcagccagc acttgagggt tcaaaacctc gataagttcc
     1801 tcaactgtca atgcttccag gccacgtcgc tcgggtaaaa ccccgaagac cggcacgcca
     1861 agtctctcga gggcaggtac tgcgtcctga gtgacaaact gcctggcttc gtctggaacg
     1921 cggttgataa tcgccccaac cagcaacttt cccagacgga aggtcgctgt aagagcgtca
     1981 tccatcaagc gcactggatc ctggtatttg attaacacta ataccggact gcccagcgcc
     2041 tcagccaccc gcggggttga aaggtcaaag atgtatcctt cccgcaggct agcgccccct
     2101 tcaacgagaa gcatgtcgac gtttgcgcca atggattgcg caacagcttt gacgcggtcg
     2161 agaagatctt ccggttgagg ctggtgaagt cgttgcctta acgcctcgct cgttactacc
     2221 actggagaga gctcccaggg ttgtgcttca aggcctaaag cctcgattac gaatgcggcg
     2281 tcttcgtcag ctacatgacc gcttactcgc cagggttgca gactgaaggg ttttaaatag
     2341 ccgaccttat atccgtctga ttggagcttc ttacctagtg ccaagcaagt ggctgtctta
     2401 cctgaatagc gttcgaccga ggtgatatag agtgatttca taggatcttc ctatcagcat
     2461 gagtatatga ggtcgccttc gccaatttca cgcctctttg ctaagagggg tgggtagggg
     2521 ggaggttggg gtttgcgagg cttccttgtg agaatcttga agcacaagtc gcatatcaat
     2581 agcaatggct ccctgaccat gtggatacac cattaatgga ttgatgtcta actcggcaat
     2641 ctcggggaaa tcttgcacaa gttgcccaat gcgcatgatt gtatctagga tggcttcttt
     2701 atccatgggc ggtttgccgc gcacaccatc caggagggcg tgggcacgaa tttcgccgat
     2761 catcgcctca gcctcctctc gagagaaggg ggcaatgcga aaagtcacat ctttaaggat
     2821 ttctacgtag atgccaccca agccgaaggt gaccagcgga ccgaactgtg gatctcggtt
     2881 catgcccacc agaacttcca gtccacccgc tggggccatc tcttgtacca ggcatcccca
     2941 aatccgcgcc tcgggtatgt agcgctgggc tcggtagacc atcaactcaa aggcatcccg
     3001 aacagcttca gcgttttgca gaccaacctt tacgccgccc acgtcggtct tatgcaggat
     3061 atctggtgaa gcgattttga gcactacagg gtagccgatc tggctggcaa tttcaatagc
     3121 gcgttcaggt gaagaggcaa tctctgagcg cgggatgtgc aagccatatg cttgcaggat
     3181 gtcacgcgcc tcggaatcac cgatcgttgt gcgcttctct gaacgaactc gattgatcac
     3241 cgattggacc agcttgatat ctgcttcaat gggttgagtt tggctggtct gcctgctttt
     3301 gatctcctga tatttggcca tcgcgcgaaa agcgtccgct gcccgctcgg ggaagggata
     3361 atttggaacg ccgaagcgat tgaggatttc aatgcccttc tcgactcgcg cctctcccat
     3421 aaaacatgcc aggataggtt tatccgtcgc atcagcgagc gcacctatgg cttcggcggt
     3481 ggcctcgata tctgtcattg cctgtggagt gagcactatc ataagaccat cggtgtttgg
     3541 gtcagcgata actttttcca tggcaaattg atagcggtca gctcgcgcat cgcccaacac
     3601 atctacgggg ttcgccgcgc tggctgcatc cggcaagtat tgttccaaag catggatggt
     3661 ttcgatttcg aaacgcgcca tgctcagacc agcgcgctcg agggcatcgg tagctaaaat
     3721 gcctgggcca cccgcgttgg ttatgatcgc aatacgattg cccctaagtt caggctggta
     3781 gccgatcgct aaggccatgt cgaacagggc ttgcatggat tcggcgcgca agatacccgc
     3841 ctggttaaaa gctgcctgat aggcttgctc cgagccggct agcgagccgg tgtgcgatga
     3901 gaccgctcga gaaccggatt gggtgacacc agacttgatg gcgatgaccg gttttttggg
     3961 ggtgatttgg cgggcgacgc ggataaactc ttccccgtta ggcatgcctt cagtgtagat
     4021 caaaatgacg cgggtgtgct catcgtcttt ccaggcttgc aggagatcga tttcgctaac
     4081 atcggcttta tttcccaggc tgacgaactt tgagagcccc agccggcccg ccaaggcgat
     4141 atccagtacc gcagtgccaa gcgctcccga ctgggacatg aaagccattg ggccagaagg
     4201 cggtgttcca gcagaaaagg atgcattgag tggcgtgaaa gtgtcgataa cgcccaggca
     4261 gttcgggccg atcaagcgca gattgtactc tttggcgatt tggatgagct cacgttcgcg
     4321 ctcgaatcct tccataccgg cttcacggaa gccggcactg atcacaatcg cagccgggat
     4381 gcctttctgc ccgcactcac gcaatgcatc gggcacttga gggtagggga taacgatcac
     4441 taccagatcg atggcagcgg gtacatccag cacggagggg taagctttgt gtcctaagat
     4501 agagttagcg cctggattga tggggtaaac catcccgcgc tgcgcgtagc caccatcaac
     4561 aagatttttt agtaccgcat ggcctaactt ggacgggtta ctcgatgctc caatgacggc
     4621 taccgagtct ggttgaaaga acggtttcag catatcaggt atattacttc tcggaaatca
     4681 ggattaatac gatagtccgc tcgtcaaggg gattgacttg cagggttatg gctgcggcgc
     4741 ggtcaggttt ttcgtaattc agcgcggcca tattctcggt ttctaccgtg cccttatcaa
     4801 cgaacgtcca gcccatgaca ggcatttgat tcttgtagaa ttccaccacc tcaggcaagc
     4861 tcagaggggt catataggag accatgaact cgttgcccag gaaattgtct gtttctccat
     4921 tgacgatggg tatgtccggt ggtgcctcat ccatcgagcc tgctatctgt gtcatggctg
     4981 ccttggctgt ctcctgcagg ccggggcctt cttgagtggc aacagctttg gcagtttcta
     5041 tgacgcttgg accttcctga gtcgccacag cgatggcggt ctgtagcaca ccgctctcac
     5101 caacctcggt tgccagtgct tgggcggtct ggatcatttc gctgcctccc acctcagtga
     5161 ccaaggcgcg cgcggttccg atgatgttta atccttcctt ggcttcggtg gcaacggact
     5221 caactgtagc cttagtctcg ccaatctcgg acaccaattg gcaagccagg gtggcaatca
     5281 acagtgtgac tatcaccaaa gcaaagcgaa tcttttggaa agtcattaat cctcttgtct
     5341 aattattgaa gctttatctg ccactgtgcg catatttaca caccacgcac aaattataac
     5401 ggcattcgct ggcatcacac atctctactg cccccggcta gttgaaatgg atccaggtaa
     5461 tgaactctct agtgtgagca gtgagctaga tcatattatt acctagggct aattattatg
     5521 agctgcctgg ggctcgattt gctgtattgc ccttcgtgtt gctgtctttg agcgtttcag
     5581 ctaacttggc gaattctccc aatttctgcg tgatggcgtg attaaatgtg cccgctgggt
     5641 aagttccgtc tggctgaagc tcgccagcct ccatctcggt gagcaggtgg atgccctctt
     5701 cgatggtatg aatcggccag acatggaatt gtccatcttc gacagcctgg actacctcat
     5761 cgcgtaacat caggtttcct acgttgctta gcgggatgat cacgccttgc tctccggtca
     5821 gccctttggt cttgcaggtc aggtagaatc cctcgatctt ctcattcacc ccgccgatgg
     5881 cttggatttg gccgcgttgg ttgatggagc ccgtcatggc acggtcctgg cgcaggggaa
     5941 tgccggcgat ggctgaaagt aaggcgaaaa gctctgcggc tgaagcgctg tcaccttcaa
     6001 tttcatcata cgattgctca aaggtcaggc tagcagagag gccaagcgtc tgcttgcgcc
     6061 cgtagcgact gcctaataac ccgctgagga tcagcacgcc tttcgtgtgg accggaccac
     6121 cgagctttgc caggcgctcg atgtccacca cgccgccttt gcccgcgtaa gccaccgcag
     6181 tgactcggct gggacgacca aaggcataat ctccgagcgg aagcactgag agcgcgttga
     6241 tctgaccgat aacactgcct gtcaggtcga tcatcagcgt accgatggca aacaactcct
     6301 ggatacgctc ctcgatcaaa ttgctgcggt atatgctctc ctcaatcgcc tttttgactg
     6361 cttcgccgct cactatgatt gacttctgag cgcttttcgc tgatttactt ttgcgcttct
     6421 tgggcattcc ggcttgctca tccgcagcct tgtctgcttg ctcggcccaa tatgctgctt
     6481 cacgaattaa gtcagcgatt ttgccgaaac gcgtagttag cctattttgg tcttcggcca
     6541 tacgggcgct atgctcaatg atgcgcgcta ccgctgagcg atcgaagggt gacaattggt
     6601 tgtcatctaa taccgacttg acaaacaggc catattcctg ctcggtttcc aatgtgcgct
     6661 tcattgtcgt ggcaaattcg gcgcgcactt taaacagctt ggcgaaatct tcatcataca
     6721 tgcgcagcag ataatatagc accggtgtgc ctaccagcac gatcttaacg ttcagcggga
     6781 agggctcggg ctccagagtg acggtactga gcagccccag ttgctgtcca agctccacaa
     6841 tacgaatttc gctgtcacgc agaacacgct tgagcccttc ccatgcgtac ggattaatca
     6901 ggatgtcgcg agctgggatg accagatagc ctccattggc gcgatgcagc gcgccagagc
     6961 ggatcatgga aaaatccgtc cggcttgcgc ccataattac ttcatgttcg atgcgaccca
     7021 ggagattggt gtaggacggg tagctttcca ggatgaccgg ggcgccttta agatcgctgt
     7081 tatccacaag cacgttgaca tcatagcgcc cgagccaatc ttgttttgcc tgctggctgc
     7141 ttgaattgct agacgaagga ctgccctcgc tccggcggaa ttggtcggaa tgctcaacga
     7201 tgtctttctg gatggcttcc aggtgcgtca agacgctttc taccccctga tatttgtttt
     7261 gcaggttctc catcaatggt ctgagc
//
LOCUS       DBNGSX010000605         5687 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1106162, whole genome shotgun sequence.
ACCESSION   DBNGSX010000605 DBNGSX010000000
VERSION     DBNGSX010000605.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5687)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1106162"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="AC16CB_17525"
     CDS             complement(<1..1285)
                     /locus_tag="AC16CB_17525"
                     /inference="COORDINATES: protein motif:HMM:NF012263.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HIR3344326.1"
                     /translation="MAIVPPPTIRAAEISTPTPTPSVSSKLQRPVMPDDPLLADKGSL
                     SYWLICLACHGDKGQGLTEEFRREIFGEDMNCWQSKCHASNHPQEGFDLPRVVPAIVG
                     PGTLSRFLTAEDLNQYILSAMPWWNPGSLSSNEAWELTAYILRENGSLPQNVEFIPQT
                     ASSAPVHLPIRSRTNEQSWQWVFLISMTIVAGALIAGSVVKSGATAAQPVKGATRPSF
                     MHHLHPPSIPLPQARWRYTLGAGGLAIYLSLVLGVTGILLMFFYIPTPEHAGPSIQTI
                     TFLVPFGSLIRGIHYWAAQALVIVALIHLLRVIFTGSFVTPRRFNFFIGLGLLVLTLF
                     LDFTGYVLRWDEGIRWALTVGTNLLQTIPLIGTQVYGFVIGGDKPGLAALTRFYAWHI
                     FGLTLVMVVLIAWHIFRVRRDGGISAPSPEQRGDTR"
     gene            1730..2374
                     /locus_tag="AC16CB_17530"
     CDS             1730..2374
                     /locus_tag="AC16CB_17530"
                     /inference="COORDINATES: protein motif:HMM:NF012523.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatA-like O-acetyltransferase"
                     /protein_id="HIR3344327.1"
                     /translation="MRHINMETWPRREHFKVFNTFDHPHFGMSANVDLTTFYPVVKRS
                     GIPITVAIIYVLSRAANAIPEFRYRLRGENVIEHEIVHPSITLLVDDNLFSFCTFDYV
                     ERFSEFSAKAIEKMAYVKAHPTLEDEGELDNLLFMTALPWVSFTSFMHPMQLHPADSV
                     PRFAWGKFFKDGDLLKMPLGVQGHHALMDGIHMGWCYEKVQDYLDHPGFVAGEA"
     gene            complement(2445..4313)
                     /gene="typA"
                     /locus_tag="AC16CB_17535"
     CDS             complement(2445..4313)
                     /gene="typA"
                     /locus_tag="AC16CB_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIR3344328.1"
                     /translation="MLIERTDLRNIAIIAHVDHGKTTLVDGLLRQARVFRENQHVVER
                     VMDSNDLERERGITILAKNTAIEVWDNLRKQTVKINIVDTPGHADFGGEVERVMNMVD
                     GVLLLVDAAEGPMPQTRFVLKKALEMGHRAIVVINKVDRKDAIPEAVLNQTFDLFIEL
                     GATDEQADFPVIYANAVTAQSGLTPALSPDLQHLFHAILRHIPAPRVDPDAPLQLLVT
                     SLGYDEYRGITAVGRIFAGQIKVGQALARIKVDGDMLPERARYLYVHQGLARIEVDQA
                     QAGEIVALAGLEAIAIGETLADPVNPQALPTIRVEEPTVRMTFGVNTSPFSGREGRWS
                     TSRKLRERLYEELRTNVALRVTDTSNADTLLVSGRGELHLAILIETMRREGYEFQVSR
                     PEVIMRQGEQGEMLEPFEEVHIETSPESVGVVVEMLGARRGQMLNMQNSAQNSVYLKF
                     LAPIRGLIGFRYQFLSATRGAGVMHTLFHGYLPHAGPISGRSSGSLVSWETGVATTYG
                     LKNAEERGVLFCDPGTEVYEGMVVGEHQRPGDLSVNVCKKKHLTNMRAARGDMELRLT
                     PPRRMSLDEAIEYLAEDELLEVTPLEFRLRKRVLSSHERGRLNKLEKEAPIRVLAQ"
     gene            complement(4627..5502)
                     /locus_tag="AC16CB_17540"
     CDS             complement(4627..5502)
                     /locus_tag="AC16CB_17540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344329.1"
                     /translation="MEKVSSASRSLILSIRLYQALLTVYPSEFRRAYGGPMLQVFRDC
                     SQRALHEGGTAGLLALWSRTMLDTVQTALEEHMQRGVDMSKEKFFKLSGWALMLGGLA
                     TMLGWLASTRPDYNRYNARSLPIDQYANAIEFPLIVMGMLLLSVGLIGLFLRYGQGAG
                     GFGRFSLGLGALSGAISAIGAIGLGIFDSEPWWSTFFIGMVVQYLGLTLFGVANLRQR
                     ALPRWNGLPILAGIWIPLSVVVGLIIEQGTGQWVEWPEAVFLIVWLLSMAGLAGLGYL
                     MQSDPQPADPAAAMA"
BASE COUNT         1351 a         1518 c         1496 g         1322 t
ORIGIN      
        1 gccgggtatc accgcgttgc tcgggtgaag gcgctgagat gccgccatcg cgccgcacgc
       61 ggaagatgtg ccaggcaatc aatacaacca tcaccaaggt gagaccaaag atgtgccagg
      121 cgtagaatcg ggtcaaagct gccaaacccg gcttgtcgcc cccaatgaca aagccgtaaa
      181 cctgggttcc gatgagtggg atggtttgca aaaggttggt gcctactgtt aaagcccagc
      241 ggatgccttc atcccaacgg aggacataac cggtaaagtc aagaaatagg gtcaagacga
      301 gcagtcccag cccgatgaaa aagttgaagc ggcgtggggt gacgaacgat ccggtaaata
      361 tcacgcgcaa tagatggatg agggctacga taacaagcgc ctgtgctgcc caatagtgga
      421 tgccccggat caggctgccg aagggcacca ggaatgtgat ggtctggatg gaaggtcccg
      481 catgctcagg ggttggtatg tagaaaaaca ttaacagaat gccggtgaca cccagcacca
      541 atgacagata gatggcgaga ccgcctgcgc caagcgtgta tcgccagcga gcctgtggta
      601 acgggatcga gggagggtgc aagtggtgca tgaaacttgg acgcgttgcg cccttaaccg
      661 gttgcgctgc agtggctccc gacttaacaa cacttccggc tatgagcgca cccgctacaa
      721 ttgtcataga aatcaaaaag acccattgcc agctctgttc gtttgtgcgc gaacggatag
      781 ggaggtgcac cggtgctgag gatgcagtct gtgggatgaa ttcgacattt tgggggagag
      841 agccgttttc acgcaggata tatgcggtta gctcccaggc ttcgtttgac gacagagaac
      901 ctgggttcca ccagggcatt gcgctgagaa tatattgatt taaatcttca gcggttagga
      961 atcgactcag cgtgcccggc ccgacaatgg ctggaaccac ccgtggcaaa tcaaatcctt
     1021 cttgtggatg gttgctggca tggcatttcg actgccaaca attcatatct tcgccaaaga
     1081 tttcacgccg gaattcttcg gtcaaaccct gacctttatc tccgtgacag gccaggcaga
     1141 ttaaccagta gctcaaagag cccttgtcgg ccaggagcgg atcatccggc atgacaggtc
     1201 tttgcaattt tgaggaaacc gatggtgttg gagtaggggt gcttatctct gcagcgcgga
     1261 tggtaggagg ggggactata gccatccaga ccacaagagc caggataaat aaaatgaata
     1321 gagaacctgc aactagcgta cggtactttg gattattggg agaaggggat gaattacgaa
     1381 agaacacgtt gtttttaaca gtccggtgct gtggttgctt cgaaatccgc tgttattttg
     1441 aatactccca aattgatgat aatgttcatc tcccttcgat atttaccgat tttcatactg
     1501 tggctcgaat cagtctggag aggtaaataa tactacaggg caattgcgta tgccctgagt
     1561 ggcatgcgca atcactctct tcagtggtgg gaaatcgtcc tcatgcgatt gccctaaata
     1621 atactatact ataagtgctg atcgattgtt atttcattat aataggagtt cggctgcaca
     1681 agaaatatat gccgtgcgaa agcaagcgcg tatcaatcgg gaggtgatta tgcgccacat
     1741 taacatggag acctggcccc gacgcgagca tttcaaggtt tttaacacct tcgaccatcc
     1801 acactttggc atgagtgcca atgtagacct gacaaccttc tatccggttg tcaagcgatc
     1861 tggcatccca attaccgtgg ctatcattta cgtgctctct cgagcggcaa acgcgatccc
     1921 tgagttccgc tatcgccttc gaggggaaaa cgtgattgaa catgagatcg tgcatccatc
     1981 cataacgctc ttggtggatg acaatttgtt cagcttctgc acgtttgatt atgttgagag
     2041 gttctcggag ttttctgcta aagcgatcga gaaaatggct tatgtaaaag cccatccaac
     2101 actggaagac gaaggagaac tagacaactt gttgttcatg actgcgctac cctgggtgtc
     2161 gttcaccagc ttcatgcacc caatgcagtt gcatccagcg gactctgtgc cacgcttcgc
     2221 atgggggaag ttttttaagg atggagactt gttgaagatg ccgttgggtg tgcaaggaca
     2281 tcacgcacta atggatggaa tacacatggg atggtgctat gaaaaagtgc aagactactt
     2341 agaccatccc ggttttgtag cgggtgaggc ttaagtgcgc agcctaagcc ctacctgttg
     2401 ggataatcat ccttgatctg gcttccaaca actgggtcaa accgttattg agccagcact
     2461 ctgatggggg cctctttttc caatttattg agccgcccgc gctcgtggct tgagaggacg
     2521 cgcttgcgaa ggcgaaattc aagaggcgta acttccagta gttcgtcttc agccaggtat
     2581 tcgatggctt catccagact catccggcgg ggaggggtga ggcgaagttc catgtcacct
     2641 cgggcagcgc gcatattcgt caggtgtttt ttcttgcaca cattaacaga tagatcgccg
     2701 ggtcgctggt gttcgcctac taccataccc tcatagacct cggtacccgg atcacaaaat
     2761 agcacgccgc gttcttctgc attcttcaat ccgtaggttg tggcgacccc ggtttcccag
     2821 gatacaagag agccagacga tcgtccgctg attgggcctg cgtggggtaa gtacccatga
     2881 aacaaggtgt gcataacacc tgcgccccgc gtggcggaga ggaactggta gcggaagcct
     2941 atgagcccgc ggatgggcgc caaaaacttc agatatacgc tgttttgggc gctgttttgc
     3001 atattgagca tttgaccccg gcgcgcaccc agcatttcta ccacgacacc caccgattcg
     3061 gggctggttt ctatgtgaac ctcctcgaat ggctcaagca tctccccttg ctccccctgg
     3121 cgcatgatca cttccgggcg cgagacctgg aattcgtacc cttctcggcg catggtttcg
     3181 attaatatag ccaggtgcag ctctccccga cctgatacca ggagtgtatc agcattgctg
     3241 gtgtccgtaa cgcgcagcgc cacgttggtg cgcaattctt catacaatcg ctcacgcagc
     3301 ttgcgcgagg tgctccaacg cccttcgcgc ccagaaaaag gtgaggtatt tacgccaaag
     3361 gtcatgcgca cggttggctc ctcgacccgg atggtaggca gtgcctgcgg attcaccgga
     3421 tcggctagtg tctcgccgat ggcgatggct tccaatccag ccagggcgac aatctcgcct
     3481 gcttgggctt gatcgacttc tatacgcgct aatccttgat gtacatacaa gtatcgggcg
     3541 cgctcaggca gcatatcacc atccaccttg atccgtgcaa gggcttgtcc taccttaatc
     3601 tgtccggcga agatgcgccc gacagccgtg ataccgcgat attcatcata gcccagtgag
     3661 gttaccagta attgaagggg tgcatccggg tcgacacgcg gcgcggggat atggcgcaag
     3721 atagcgtgga agagatgttg caggtcggga cttaatgccg gcgtcagacc agactgagca
     3781 gttaccgcat tggcataaat taccgggaaa tctgcctgct catcggttgc acctagttca
     3841 ataaacaggt caaaggtttg gttgagcacg gcttccggga tggcatcttt acgatcgacc
     3901 ttgttaatca ctacaatagc acggtgaccc atctcgaggg ctttcttgag cacaaaacga
     3961 gtttgcggca tgggcccttc ggcggcatcc accaaaagca acacaccatc aaccatgttc
     4021 atcacccgct cgacctcacc gccaaagtcg gcgtgtccgg gcgtgtcgac gatgtttatt
     4081 tttacagttt gcttgcgcag gttatcccac acctcgatgg cagtgttttt tgccaggatg
     4141 gtgataccgc gctcgcgttc caggtcgttg gaatccatta cacgctcgac cacatgctgg
     4201 ttttcacgaa aaacccgcgc ctgcctgagc aggccatcca ccagggtggt tttaccatgg
     4261 tccacgtggg caatgatagc gatattgcga agatcagttc tttcaattaa catactatgc
     4321 taaactttct acatctatct cagtttctat atttgaacag ataacgaccc cgaccattca
     4381 ggccggggtc gcctttcagc cactcaatat taccaaaaag cctccaattt gagaaggggt
     4441 atattaattt ttgatttaca gtattcagcc ctttgactaa agtctgggca atactacatc
     4501 aggtctattc aggcgcgggt ctacgaatgt ttgcttcttt ttggtccgtt taattaacat
     4561 tgccaagatc atttcccacc tacccagtgg atatatttga tgcttgtcac ctcggccaaa
     4621 aatcttttat gccatagctg ctgccgggtc ggcgggctgt gggtcagatt gcatcaggta
     4681 gcccaagcct gccaatcctg ccatgctgag tagccagacg attagaaaaa ccgcttcagg
     4741 ccactccacc cattgacctg ttccttgttc aataatcaag cccaccacga cggacaaggg
     4801 gatccatata cctgccaaga tgggcagccc gttccaacgc ggcagtgcgc gctggcgcaa
     4861 attagccacg ccgaacagtg tcaatcccag atactgaacc accataccaa tgaagaaggt
     4921 tgaccaccag ggctcgctgt caaagatgcc cagaccaatg gcgccgattg cactgatcgc
     4981 tccgctgagc gcgcccagcc ccaggctaaa ccgcccaaaa cccccagccc cctgcccgta
     5041 gcgcaggaac agcccgatca agccaacgct cagcaagagc atgcccatga caattagtgg
     5101 aaattcgatc gcattggcgt actggtcaat aggcaggctg cgggcattgt agcggttgta
     5161 atccgggcgt gtgcttgcca gccagcccag catcgttgcc aggccgccga gcatcaatgc
     5221 ccaaccactc agtttgaaaa atttctcttt tgacatatcc acacctcgtt gcatatgttc
     5281 ctcgagcgcc gtttgaacgg tgtcgagcat cgtccggctc cacaaagcca gcagaccagc
     5341 tgtgccgcct tcatgcagcg ctcgctggct gcagtcgcga aatacctgca gcataggacc
     5401 gccatacgcc cggcggaact cggacgggta aacagtgagc agcgcctggt aaaggcggat
     5461 ggacaagatc aacgatcgtg atgcgctgga gactttttcc atctttgcct ccatcaggct
     5521 gatcctttca ggacctgctt taaacgcgcc aggcggactt gcccttccag gcgctcggct
     5581 tccaggcgca gggcgcgctg ccccaaacca cttaagcggt aatagcgccg gcgctcatca
     5641 tccaacgctg ggtcagggcg ctcgtcgcac tcttcgatca ggccact
//
LOCUS       DBNGSX010000606         2770 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_691075, whole genome shotgun sequence.
ACCESSION   DBNGSX010000606 DBNGSX010000000
VERSION     DBNGSX010000606.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_691075"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /gene="gltX"
                     /locus_tag="AC16CB_17545"
     CDS             complement(<1..868)
                     /gene="gltX"
                     /locus_tag="AC16CB_17545"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR3344330.1"
                     /translation="MENRDKPVRVRFAPSPTGHTHLGSGRTALYNYLIARQTGGQFLL
                     RIEDTDRKRYVPGAEEELIDSLHWLGLQWDEGPDVGGPYAPYRQSKRKEIYQHHARKL
                     VEMGHAYYCFCTPQRLAQVRQEQQKLKQSTLYDGLCRKLDPDEADKRVANGESHVVRF
                     KTPKEGSITAVDRLRGPITVENHTLDDYILLKSDGLALYHLAAMVDDHLMKITHVLRS
                     SEWLPTFPLHAHIIRSFGWQEPEWVHLSVFLKPSGKGKMSKRESADLKQSGYSIFIKD
                     LEELGYLPEAVVN"
     gene            complement(881..2014)
                     /locus_tag="AC16CB_17550"
     CDS             complement(881..2014)
                     /locus_tag="AC16CB_17550"
                     /inference="COORDINATES: protein motif:HMM:NF013279.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR3344331.1"
                     /translation="MQTSSSSNWRHFDFWLFGAVVVLTIFGITMIRSAVAGNIELTTE
                     TNLVNRQITFTIVGFVAMFLTASIDYHYWSSASRVLYLGTAVALIILNVIAQTVFGSA
                     RWFQVLTINIQPSEIAKIVIILVLADFFTQNYEEVGNLKIIRNSLLYTLGIMIWVLLQ
                     PNLSTSIVIMVIWFSLLWAGGLKLKHLLLFIGIGLLAGIILVPIMIQLDIIKEYQIAR
                     VTSFLFPDPNARFGDIYNIQQALISIGSGGWFGQGYGGGSQVQLRFLKVRWSDYIFSA
                     MAEEFGFVGTLIVLAVLAFVIYRCLRAARLARDTFGALIAYGVSTLIAFQAMVNIGVN
                     LNLLPATGLTLPFVSYGGSSLLSALLGIGLVESVLLRQKALEF"
     gene            complement(2095..2364)
                     /gene="minE"
                     /locus_tag="AC16CB_17555"
     CDS             complement(2095..2364)
                     /gene="minE"
                     /locus_tag="AC16CB_17555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HIR3344332.1"
                     /translation="MNRFFDRLLGRDQKSARQAKERMKLVLIHDRTDLTQSAMEALKD
                     ELIAVISRHVEIEPEAVRIEMAQDGREQRLIADIPLRTVRRRRVG"
     gene            complement(2366..>2770)
                     /locus_tag="AC16CB_17560"
     CDS             complement(2366..>2770)
                     /locus_tag="AC16CB_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559399.1"
                     /note="ATPase; with MinC inhibits cell division by
                     blocking formation of the polar Z ring septums; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HIR3344333.1"
                     /translation="APADIVLVITNPEVSAVRDADRIIGLIEAEEKGPARLVINRIKP
                     DMVRRGDMLSADDVLELLAIELVGLIPEDENVITSTNRGLPIALNGKSRAGQAFRNIA
                     KRLSGEEVPFLELENDGNLIGRLARFFRPGGN"
BASE COUNT          684 a          717 c          734 g          635 t
ORIGIN      
        1 agttaaccac ggcttctggc aggtagccca attcttcaag atctttgata aagatagaat
       61 agccgctctg tttcaaatcg gcggattcac gcttgctcat ttttcccttg ccgctgggtt
      121 taagaaatac tgaaagatgc acccattcgg gttcctgcca accgaaagag cggatgatat
      181 gagcgtgcaa tgggaaggtt ggcagccatt cagagctgcg gagaacatgg gtgattttca
      241 tcaaatggtc gtcgaccata gcagccaggt gatagagcgc caacccgtca gatttgagca
      301 ggatgtaatc atccagggtg tggttttcta ccgtaatagg gccgcgcaat cggtctaccg
      361 cggtgatgct gccttctttt ggggttttga agcgcactac atgagactcg ccgttggcga
      421 cacgcttgtc ggcctcatca gggtccagct tgcggcataa gccatcatat aaggtgcttt
      481 gctttagttt ttgctgctcc tggcgcacct gggcgaggcg ctggggggta caaaagcagt
      541 aataggcatg gcccatctcg actaacttgc gggcatggtg ctggtatatc tccttacgtt
      601 tggactggcg atatggagca taaggaccac ccacgtcggg gccttcgtcc cattgcaatc
      661 ccagccagtg cagactatca atgagctcct cctcagcgcc gggaacgtat cgcttgcggt
      721 cggtatcttc gatgcgcagc aagaactgtc caccggtctg gcgggcaatc agatagttat
      781 acagcgcggt gcgcccacta cccagatggg tgtggccggt tggggagggc gcaaagcgca
      841 cgcgaacagg cttatctcta ttttccattg acaaaaccca ttaaaattcg agcgcttttt
      901 gccgcaagag tacgctctcg accagcccga tgccgagcaa ggcggagagc aatgaacttc
      961 caccgtaact cacaaaaggt aaggtcaagc cggtggctgg cagcaagttt aaattaacgc
     1021 cgatgttcac catcgcctgg aaggcgatca aggtggagac accgtaagcg ataagcgctc
     1081 caaaagtatc ccgcgccagg cgagcagcgc gtagacagcg gtagatgacg aaagccagca
     1141 ccgccagcac gatcagtgtg ccaacgaaac cgaattcctc agccatggcc gaaaagatgt
     1201 aatctgacca gcgtaccttg agaaagcgta attgcacctg agaaccacca ccgtatccct
     1261 gcccaaacca gccgcccgag ccaatgctga tcagcgcctg ctggatattg taaatatcgc
     1321 cgaagcgggc gtttggatca gggaataaga aactggtgac gcgagcaatt tgatattcct
     1381 tgatgatgtc tagctggatc atgatcggca caaggatgat gccggccagt aggccgatgc
     1441 cgatgaagag cagcaggtgt ttcaatttca aaccacctgc ccacaacaag gagaaccaga
     1501 tcaccataat tacgatggat gtgctcaagt tgggctggag taacacccag atcatgatcc
     1561 ccagtgtata cagcagactg tttctgatga tcttgaggtt gccgacttct tcataatttt
     1621 gggtgaagaa atcagctagc accaggatga tgacgatttt ggcgatctcg gaaggttgga
     1681 tattgatggt tagaacctgg aaccagcgtg ccgagccaaa cacggtttgg gcgatcacat
     1741 ttaatatgat cagcgccaca gcagtgccca gatacaatac tctgctggca gacgaccagt
     1801 agtgatagtc gatgctggcg gttaagaaca tggcgacaaa accaactatg gtaaaggtga
     1861 tctgccgatt gaccaggttg gtctcagtag ttaactcgat attcccggct accgcagagc
     1921 ggatcatggt gatgccgaag atggttaaga ccaccactgc cccaaagagc cagaaatcga
     1981 aatgtcgcca atttgaagac gaacttgttt gcatgaacaa tccacaactt gctgagaaaa
     2041 ttgctgccca gttagatcag gtcaatcggg cagatttctc aagcacacct gtcattatcc
     2101 cacacgacgg cgccggactg ttcgcagggg aatatcggca atcaagcgtt gttcgcgtcc
     2161 atcctgtgcc atctcgatgc gcactgcttc tggttctatt tcaacatggc gggagatgac
     2221 ggcgatcagc tcatccttga gggcttccat ggcactttgc gttaagtctg ttcgatcatg
     2281 gatgagaacc agcttcatgc gctctttcgc ctgtctggca ctcttttggt cgcgtccgag
     2341 aagacgatcg aagaaacggt tcatttcaat tgcctcccgg gcggaaaaag cgtgccagcc
     2401 gccctatcaa gttgccgtca ttttctaatt ccaagaaagg cacttcttca ccgctaagac
     2461 gcttggcaat attgcggaaa gcctgtcctg cgcggctctt gccattaagc gcaatcggca
     2521 acccacgatt ggtgctggtg atgacatttt catcttcagg tatgaggcca accagctcga
     2581 tcgcgagtaa ctccaacaca tcgtcggcag agagcatgtc cccacgcctg accatgtcgg
     2641 gtttaatgcg gttgatcacc agacgtgctg gccctttttc ctcggcttcg atcaagccaa
     2701 tgatgcgatc cgcatcccgt actgccgata cctcgggatt ggtgattacc aagacgatgt
     2761 cggcgggtgc
//
LOCUS       DBNGSX010000607         7693 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_621420, whole genome shotgun sequence.
ACCESSION   DBNGSX010000607 DBNGSX010000000
VERSION     DBNGSX010000607.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7693)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7693)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7693
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_621420"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3088)
                     /locus_tag="AC16CB_17565"
     CDS             complement(<1..3088)
                     /locus_tag="AC16CB_17565"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3344334.1"
                     /translation="MTYQLFIVVVFALIPIFAIRWYKQPFIDLLTAFVIPYLVGLVYL
                     GTSIWIYSLRRSNTSSRLFSFLTASIGLNIAGFFDLFTTNYLATLWTANLMLIGGALI
                     NFALLFPEEARLLSRYPYLRWVGFVPSAIFVGYTLLTQKPHSYSNLLQLGYLYTWFAA
                     GVFVARITHQRLNSASPITREQSRLIIWGVVISFTPILVWLLSSIFKPGQEFSSIILV
                     PTVTFPVLTAYAMLRYRALNTDYLLSKTTLYATLSIVSAAGYALAVSGLSLVFGNLLP
                     PNHPLLVGLLVFLLAMLLNPLRLKLQRFVDSIFFRKQTDYQDRLQAFRRELTQTIELP
                     AIVQSLRTYIAKSLYPNQIHIFIRDEQSGQYAPEPGGDGKPTTDIRFPPNSVLVQILE
                     RRHGSIYLGGTETLPTLLQTERARLALLAVQLFVPLPGQSSLIGWLSLGTRQSGEPYS
                     NNDLRYLESLCDQAALAIERAQGVANLEQRVHEMDVLTRIAQGVNITLDFDDILELIY
                     AQTNVLIPTRDFRVTLRNQSNQALYYAFFLENDERLQGRENNNLLDGQTLEEVVIRSQ
                     RALLTDDYERESRGRGILAHEPDLFSWIGVPLNSGADTIGAISLGSRDPSKIFTQHQA
                     NLLQAIADQAAGAIIKARLLQESERRARQLATLNEVGKSLTSTLELRPLLNKILKSAT
                     DILNCEAGSLLMVDEHTSELVFEVTVGPVAAELIGQHLPPGTGKVGEAVQNRQPIIAN
                     DVRQSQGWFDQTDKKTGFITRDLLAVPMMLHERVIGVIELINKNDGSPFNQDDQELLA
                     TFASQAAIAVENARLYTQTDKALSARVEELSVMQRIDRELNVSLDLERAMRITLEWAM
                     RQSRTDAGLVGLLEEVIDDQATAIRVIAFQGYDAELSQSPDQDDNSPGARRLPVQMES
                     IYKAIESGQPYAVIQTENSSDETAADDPSQPPLILLSRGKSQVVVPIRRTGDVIGIIL
                     LESEHPTAYQEEIIAFLSRLSDHAAIAISNAQLYADLQAANIAKSDFVSLVSHELKT"
     gene            complement(3261..4475)
                     /locus_tag="AC16CB_17570"
     CDS             complement(3261..4475)
                     /locus_tag="AC16CB_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HIR3344335.1"
                     /translation="MSIAYPGHQKPEINFILREGHPDFSDLPWHLPLSNWAEICPRLE
                     DVPRGLTRHTVVFVNYDGILFALKEMPLRLAEKEYNTLIQIGALRIPAVTPIGLVSIQ
                     IPHDQIGVLITRYLEHSIPYRSLFMTGNLVRYREHLLDAISGLLVQLHLLGTYWGDCS
                     LSNTLFRRDAGALQAYLVDAETATIYPERTPASMRHQDLDIMEENVDGDIADLATAHL
                     LSAGIPTQDTGAYIRLRYQKLWEEITQEQIISSGESYRIQERIRALNELGFSVSDMEM
                     LNTESGEQLRLRLVVSDRNFHRNQLFDLTGLEAEERQARQMMNEIQELRANLSHTHNR
                     STPLSVAAYQWLLEIYQPTIQRLNPLIKKDMNLAELYCQVLEHKWFLSEKAQRDVGHS
                     AAIDDYLNKSTE"
     gene            complement(4441..6258)
                     /locus_tag="AC16CB_17575"
     CDS             complement(4441..6258)
                     /locus_tag="AC16CB_17575"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3344336.1"
                     /translation="MSFFSNLDTERYDRQYPDRQLVRRMLDYFRPHRGRLGIVAALLL
                     IISLAGAAQPWIVARGVDLLRADLALRNIILLGGTILVIGIGNWLANWLRRRITGRVI
                     GDTVLTLRTEAFRAAADHDLSFYDEFSSGRIVSRITSDTQEFGQVVMLITDLISQFAQ
                     AFILGVVLFNIDLRLSIYLFGFIPVLFVTALGFRRLARTVTRRGMRAMANVNAAIKET
                     VSGIAIAKNFRQENSIFAEFNEANQQSYRVNVQRGFVLSLVFPSLNALGGIATAILVF
                     VGGLSAAQGAVTIGAWFLFLQSLDRFFFPVLNLSAFWAQVQSGLSASERVFALIDAET
                     AVVQTNNQIVKQLKGEISFEQVRFRYNEKEQVLEDFNLLIPPGETIALVGHTGAGKSS
                     IAKLIARFYEFQNGNILIDGRDIRTLDLNSYRRQLGIVSQVPFLFSGSVLENICYASQ
                     DGVDFCDVEKIAMQIGEGEWLETLPQGLQTQVGERGVLLSMGQRQLISLMRVLVHKPV
                     IFILDEATASIDPFTESQIQQALNMILANTTSIMIAHRLSTVIAADRIIVLDQGRIIE
                     EGNHASLLAAKGQYASLYNTYFRHQSLAYVDSLSWTSKT"
     gene            complement(6307..>7693)
                     /locus_tag="AC16CB_17580"
     CDS             complement(6307..>7693)
                     /locus_tag="AC16CB_17580"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3344337.1"
                     /translation="TMARATNDVREINLMFSPGINLVIGSASFLIMPLFLAPRYHPSL
                     IITPALFMIGYFLSLWQYLRELDPVTGEVRKSFGHLNSRLAEALDGIETVKGMVQENS
                     EVGLFTRYARGYRDAFIRQIDIEARFLPLLLLGLAEAGGLLHAILLFWQGQLDVGAVV
                     AYFGLLQLFGFPTFVSLFSYSQVSLGIASARRILELINRENNLDQNVSGYSGSMRGEI
                     EFRNVTFSYGDEEPVLHEVSFKVQAGQTVAIVGQTGAGKTSLVRLVNRTYDVAGGQVL
                     VDGIDVRQWNLEALRRNISMIEQDIFLFSRSIADNVAFGKQEASMQSIIQAANAAQAN
                     DFIESFKDSYQTVIGERGVTLSGGQRQRLALARAFLTDPRILILDDSTSAIDSATEDK
                     IQRAIYTAARGRTTLIITHRLSQIRWANLIVLLRKGRVAAIGTHEHLMQTSESYRRIF
                     VSRETDPDNNG"
BASE COUNT         1958 a         1815 c         2028 g         1892 t
ORIGIN      
        1 gtgtcttaag ctcgtgtgaa accagggaaa caaagtcgct cttggcgata ttggcagctt
       61 gtagatcggc atagagttgg gcgttggaga tggctatagc ggcatggtcg cttagacgcg
      121 agagaaatgc gatgatttct tcttggtaag cggtgggatg ctcactttct aggagtatga
      181 tgccgatcac atcgccggtt ctgcggattg gcaccacaac ctggcttttt cccctagaca
      241 ataagatcaa aggcggttgg gatgggtcgt cggctgctgt ttcatcactg gagttttcgg
      301 tttggataac ggcgtacggt tgaccggatt ctatggcctt ataaatggat tccatctgga
      361 caggtaaacg tcgagcacct ggagaattgt catcttgatc tggagattgg ctgagctcag
      421 catcatatcc ttgaaaggca atcactcgaa tagccgttgc ttgatcatcg atgacttcct
      481 cgagcaaccc taccagacca gcatcagtgc gcgattggcg cattgcccat tccagggtaa
      541 tgcgcatggc gcgctcgaga tctaggctga cgttgagttc gcggtcgatg cgctgcatga
      601 ccgagagctc ttctacacgg gcagagagag ctttatcggt ctgggtgtac aagcgggcat
      661 tttctacggc aattgcagcc tggctagcga aggtagctaa cagttcttgg tcgtcctgat
      721 tgaaaggtga accatcattt ttatttatta actcgatgac accgataacc cgctcgtgca
      781 acatcattgg gacagccagt aaatcgcgcg tgatgaagcc ggtttttttg tcagtttgat
      841 caaaccaacc ttgtgattgt cgcacatcgt tggcgatgat gggttgcctg ttctgcaccg
      901 cctcacccac ttttcctgtt ccaggtggta agtgttgtcc gatgagctca gcggcgactg
      961 ggccaacagt aacctcaaaa acaagctcag aagtgtgctc atcgaccatc agcaggctgc
     1021 cagcctcgca attcaagatg tcagtagcgc ttttcagaat tttgttcaga agtggtcgca
     1081 gctccagggt ggaagtaaga cttttgccaa cctcgttgag ggtagctagt tgccgagctc
     1141 tccgctcgct ctcctgaagt aggcgagctt tgatgattgc tcctgcagct tggtcagcta
     1201 ttgcctggag caaattggcc tgatgctggg taaagatctt ggatggatcg cggcttccta
     1261 ggctaatggc accgatggtg tccgctccgg aattcaatgg cacaccaatc catgagaaaa
     1321 gatccggttc gtgtgccagg atgccgcggc cgcgggattc gcgttcataa tcgtcagtca
     1381 acaaggcacg ttgactgcgt atcactactt cttcgagggt ttgtccatct agaagattgt
     1441 tattttcgcg gccttgcagg cgttcatcgt tttctagaaa aaatgcataa tataaggctt
     1501 ggttggattg atttcgcaac gtcacccgaa aatcgcgggt ggggataaga acgttggtct
     1561 gagcgtaaat caactcgagt atatcgtcaa aatctagggt gatattcact ccctgggcaa
     1621 tgcgtgtgag cacgtccatc tcatgtacgc gctgttccag gttggcaacc ccctgggcgc
     1681 gctcaatcgc cagggctgct tgatcgcaga gggattctag gtaacgcagg tcgttgttag
     1741 aataaggttc gcctgattga cgcgtcccca agctcaacca gccgatcagg cttgattggc
     1801 ctggtaaggg cacaaaaagc tgcacagcca gcaatgctaa acgagcgcgt tccgtttgca
     1861 gcaaggtggg cagcgtctcg gtgcccccta aatagataga gccatgccgt ctttcaagaa
     1921 tttgcaccaa tacactgtta ggcggaaatc ggatatctgt agtgggtttc ccatctccac
     1981 ctggttctgg agcatattgc ccgctctgct catccctgat gaaaatatga atttgattcg
     2041 ggtataagct tttagcgata tatgtgcgca atgactgcac gatggcaggt aattcaatgg
     2101 tttgagtgag ttcccgtcta aatgcttgta ggcgatcctg gtagtcagtt tgtttccgaa
     2161 agaatatcga atcgacaaat cgttgtaatt tcaagcgcag agggttgagc agcatggcaa
     2221 gaaggaatac taacaagcca accagcaagg ggtgatttgg gggaagtagg ttgccgaaga
     2281 ccaagctgag cccgctaact gctaatgcat atccagctgc ggagacgata gatagcgtgg
     2341 catatagggt tgtcttgcta agtaaatagt cagtattgag ggcacggtaa cgcagcattg
     2401 catatgctgt cagcaccgga aatgtgactg tgggaaccaa tattatggat gagaattctt
     2461 gaccaggctt gaatatggaa gacaataacc aaaccagaat aggggtaaaa gaaataacca
     2521 ccccccatat gatcaggcgg gattgctcgc gagtgatcgg cgaagcggag tttaatcgtt
     2581 ggtgtgttat ccgcgccacg aatacccctg cagcgaacca cgtgtacaga tatccaagct
     2641 gcaataaatt ggaatatgaa tgcggtttct gagtgagcag cgtgtaaccc acaaaaatag
     2701 cgctaggcac aaaaccgacc cagcgcaggt atggatatcg cgagagcaat cttgcttctt
     2761 cggggaaaag gagggcaaag ttaatcagcg ctccgccgat taacattagg ttcgcagtcc
     2821 acaatgtagc taaataattg gtggtaaata aatcgaaaaa cccagctata tttagaccga
     2881 ttgaggccgt tagaaatgaa aacaacctgc tactggtatt gctgcgcctc aaactgtata
     2941 tccagatact ggtgcccaaa tagaccaacc cgacgagata tgggatgaca aaggcagtca
     3001 gcaaatcgat aaatggttgt ttataccaac ggatcgcgaa aatcgggatt aaggcaaaaa
     3061 caacgacgat gaatagttgg taggtcaaaa cagccacagg gcctgacctg tgttgcaaga
     3121 aagcaagtaa tgatgggcgc gcgaacctta gcgttagacg catagagtaa ttttatggcg
     3181 taatacgcca gcggtcaatt tttggaggcc ctgtttagcc ttatggtttt gtaaggtatg
     3241 ccgcgccttt gctcattgaa ttattctgta gatttgttaa gatagtcatc aatggcagcc
     3301 gaatgaccca catctcgttg ggctttctca gacaggaacc atttatgctc taatacctga
     3361 cagtacaatt cagccagatt catatccttc tttatgagag gattgaggcg ctggatagtt
     3421 ggctggtata tctctagcag ccactggtac gccgcgacgc tcaggggtgt gctgcggttg
     3481 tgcgtatgcg agaggttggc gcgcaattcc tgaatttcgt tcatcatctg ccgggcttgc
     3541 ctttcctctg cctccaatcc ggtcagatcg aaaagttgat tgcggtggaa gttgcggtct
     3601 gagactacca agcgcaggcg gagttgttcg ccgctttctg tatttagcat ttccatatca
     3661 cttaccgaga agcccagttc gttcaaggcg cgtatgcgtt cctgtatgcg atagctctct
     3721 cctgaggaga taatctgctc ctgagtgatc tcttcccaca acttttggta acgcaagcgg
     3781 atgtaagcgc ccgtatcttg tgttggtata ccagcggaga gtagatgggc agtagccagg
     3841 tctgcaatat caccatctac gttttcttcc ataatatcca gatcttggtg gcgcatgctc
     3901 gctggggtgc gttcgggata tatcgttgca gtctctgcat ctaccaggta agcttgcagt
     3961 gcgcctgcat cgcggcggaa gagcgtattg gaaagcgaac aatcgcccca ataggttccg
     4021 agtagatgaa gttgcacaag caacccagag atggcgtcaa gtaggtgttc gcgataacgc
     4081 acaagatttc cggtcataaa aagcgaccga tacgggatag aatgttccag gtagcgtgta
     4141 atcaagaccc caatctggtc gtgcgggatt tggatgctca ctaatccaat cggtgtaaca
     4201 gcgggtatgc gcagcgcacc gatttggatc agagtgttat attctttctc agccaggcgg
     4261 agcggcatct ctttcaaggc gaataggatg ccatcgtagt tcacaaagac caccgtgtgg
     4321 cgagtcagac cgcgcggaac atcctctaag cgcgggcaaa tttcagccca atttgaaagg
     4381 ggcaggtgcc agggcaggtc gctgaaatcg gggtgaccct cacgcaggat gaaattaatc
     4441 tcaggttttt gatgtccagg ataagctatc gacataggca aggctctgat gacggaaata
     4501 tgtattgtac aggctagcgt attgtccttt agctgctagc aaactggcat gattaccttc
     4561 ttcgatgatg cgcccctgat ccaggacaat aatgcgatct gcagcgataa ctgtagataa
     4621 gcggtgagct atcattatgc tggtggtgtt agccaggatc atatttagcg cctgttggat
     4681 ttgggactcg gtgaatggat cgatgctggc ggtggcctca tctaatataa atatcacggg
     4741 tttatgcacc aggacacgca tcagggagat caactggcgc tgccccatgg atagcaacac
     4801 gccgcgctca ccgacttggg tttgcagacc ttgaggcagc gtctcaagcc actccccttc
     4861 gccaatttgc atggcgattt tctcgacgtc acagaaatcc accccgtcct gcgatgcata
     4921 gcagatattt tccagcacgc tgcctgagaa gaggaagggt acctgggaga caattcctag
     4981 ctgacgcctg tagctattca ggtccaacgt gcggatgtct cgcccatcga ttaatatgtt
     5041 accattttgg aactcataga aacgagcaat cagcttggcg atagaacttt ttccagcccc
     5101 tgtgtgaccc accagagcaa ttgtctcgcc aggagggata agcaaattga agtcttccaa
     5161 aacctgctct ttttcattgt agcgaaagcg aacctgttcg aaggaaattt caccctttag
     5221 ttgtttgacg atctggttat tggtttggac tacagctgtc tcagcatcga tcagggcaaa
     5281 gacgcgctct gatgctgaaa gcccagattg gacttgggcc caaaaggctg acaagttcag
     5341 aacggggaag aaaaatcggt ccaggctttg caagaacaaa aaccaggcgc cgatggttac
     5401 tgccccctga gcggcactca agcctcccac aaacaccaga atcgctgtag ctatgccccc
     5461 cagggcgttc agcgacggaa ataccagcga cagaacgaat ccgcgctgga cattcacgcg
     5521 gtatgactgt tgattggctt cgttgaactc ggcaaatata ctgttctcct ggcgaaagtt
     5581 ttttgcgata gcaattccac tgactgtctc tttgattgcg gcatttacgt tagccatggc
     5641 gcgcataccc cgtcgggtga cagtgcgggc taagcggcgg aaacccaacg ctgtaacaaa
     5701 aaggaccggt atgaaaccaa atagataaat ggacaggcgg aggtcgatat taaacagaac
     5761 tactcctaga ataaaagcct gggcaaactg ggagatcaga tcggtgatca acatgaccac
     5821 ctgaccgaac tcttgtgtat cggaggtgat gcgcgaaacg atgcgtcctg aagagaattc
     5881 gtcgtaaaag gagagatcat gatctgcagc tgctcggaat gcctcggtac gcaaggtgag
     5941 cacagtatca ccgattacgc gccctgtgat acgccggcgc agccagttgg ctaaccagtt
     6001 acctatgcct ataactaaga tggtgccacc caatagtatg atattgcgca gggcgaggtc
     6061 agcgcgtaac aaatccaccc cgcgggcgac gatccagggt tgagcagccc cagccagcga
     6121 gataatcaga aggagcgcgg ctacaatacc taaacgccct ctatgcgggc ggaagtaatc
     6181 cagcatccgc cgtactagct gacggtcagg gtattgtcga tcatagcgct cagtgtccag
     6241 attagaaaaa aaactcatga tctaactcta agggcaggtc acatagtaaa gcatacaaag
     6301 aagtctctag ccgttgttat ctggatcagt ttctctgctg acgaatatcc tccgatagga
     6361 ttcggaagtt tgcattaaat gctcgtgggt gccaattgca gcgacgcggc cctttcttag
     6421 caggacgatc aaatttgccc accgaatttg ggatagtcta tgtgtgatga tcagggtggt
     6481 tcgtccgcgt gctgcggtat agatagcgcg ttggattttg tcctctgttg cgctatcgat
     6541 tgcgctggtc gaatcgtcca gaattaagat gcgtggatcg gtgaggaagg ctcgcgccaa
     6601 cgccaggcgc tgtcgctgac cacccgagag agtaacgcca cgttcgccga taacggtctg
     6661 gtagctatct ttgaacgatt cgataaaatc atttgcctga gctgcattgg cagcctggat
     6721 gatcgattgc atcgaggctt cttgctttcc aaaggctacg ttatcagcga ttgagcgtga
     6781 gaaaaggaat atatcctgtt ctatcatgga tatattgcgt ctcaacgcct ccaggttcca
     6841 ctgccgcaca tctattccgt ctacaagtac ttgaccgccc gccacatcat aggttcggtt
     6901 aaccagcctg acaaggctag tctttccagc gccggtctgg cctactattg ctactgtttg
     6961 ccctgcttga actttaaaag agacctcgtg gagaactggt tcttcatcgc cgtaagaaaa
     7021 tgtcacattg cgaaattcaa tctctcctcg cattgaacct gagtagccgc ttacattttg
     7081 gtctaggttg ttctcgcgat taataagctc taatatacgg cgtgcgctgg ctattcctaa
     7141 tgagacctgg gaataggaga aaagcgagac aaaggttggg aagccaaaga gttggagaag
     7201 tccaaaatag gcgaccactg cacccacatc tagttgccct tgccaaaata gcaggatggc
     7261 atgtaataag ccaccagctt cggccagacc taacaataaa agtggtaaaa agcgggcttc
     7321 gatatcgatc tggcggatga atgcatcccg atacccgcgg gcgtagcgcg tgaaaagacc
     7381 tacctcgctg ttctcctgga ccatcccttt aacggtctca attccatcca aggcttcagc
     7441 cagtcggcta tttaaatgcc caaatgattt ccgaacttca ccagtgacgg gatctaattc
     7501 gcggaggtac tgccacaagg agagaaagta tccgatcata aacaatgctg gtgtaatgat
     7561 cagggaaggg tgataacgcg gggcgaggaa tagcggcata ataagaaaac tagccgagcc
     7621 aatcaccagg ttgatgcctg ggctgaacat aagattgatt tcgcggacat cgttggttgc
     7681 ccttgccatg gtg
//
LOCUS       DBNGSX010000608         8779 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1729588, whole genome shotgun sequence.
ACCESSION   DBNGSX010000608 DBNGSX010000000
VERSION     DBNGSX010000608.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8779)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8779)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8779
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1729588"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1825)
                     /locus_tag="AC16CB_17585"
     CDS             complement(<1..1825)
                     /locus_tag="AC16CB_17585"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HIR3344338.1"
                     /translation="MALGGYADKVAHIDLTKGQVTYDAIPEDWKLKYIGGRGVGVKYV
                     FDNGPQVDPLSPDNLLSFMNGPLTGTEVNLSGRIAIVTKSPLTGTITDSHHGGWSGAR
                     LRWAGFDGLNFKGKAEKPVYVFVKAGKVEIHDASDVWGKGVHDTVKYFKDKYGEKDLS
                     VITIGQAGEKLSRFACWVNENDRASGRGGTGCVGGSKNLKAVVIKATKKMPKAVDREL
                     WKEAHANALAQLKDPKYITSPRKGGLSTLGTNVLMNMVNVIGAMPTKNSQLTYFEDHE
                     LISGEYVRDNILVNNPTCHACPVACKKEVIIKDGPYAGLQMESVEYESAWALGANCDN
                     SDVASVAKLIDQCNDYGFDTIEMGNVLSVYMEASEKGYTNGAGGLKWGDHPAMVETVR
                     KIASREGIGNVLAEGAERAAKTFGHPEIAMTVKGMAIPAYDPRGIKGMGIGFATSNRG
                     ACHLRGYTPAAEVVANVLGSTDLTDPLEWKGKGQLTVIFQNVHTMTDCLDICKFATFA
                     EPLDSFAAQYTAITGVPADAGMLLKAGERVYNLERYYNNLIGFVEGSDSLPERFLKEP
                     STGPGSKGHVCELPEMLEEYYEARGWRNGVVTEEKLKELELI"
     gene            complement(2238..2432)
                     /locus_tag="AC16CB_17590"
     CDS             complement(2238..2432)
                     /locus_tag="AC16CB_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIR3344339.1"
                     /translation="MMTTRQALRRSRSNRVFMGVCGGLAEFFGISAFWFRLAFIIALI
                     PGGVPGILIYLLLWLIIPSE"
     gene            complement(2490..3134)
                     /locus_tag="AC16CB_17595"
     CDS             complement(2490..3134)
                     /locus_tag="AC16CB_17595"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR3344340.1"
                     /translation="MTIYNDQISQYTTNLFAIHDKGLQRAWDETPRKGLPAISIKPEE
                     GRFLQFLTRACGAKKALEIGTLGGYSGIWIARGLVPGGKLITLELESRHAEIAREHFE
                     FAGVGESIEIRLGDAHHALEQLSSEAPFDFVFIDAEKTGYPKYLAWALEHTRVGGIIA
                     AHNAYRKGSVAGLAEPDAYTETMREFNISIATNPRLMSTIYPAGDGTLVSVKVA"
     gene            complement(3154..4071)
                     /locus_tag="AC16CB_17600"
     CDS             complement(3154..4071)
                     /locus_tag="AC16CB_17600"
                     /EC_number="3.5.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000118 - histone deacetylase complex
                     [Evidence IEA];
                     GO_function: GO:0004407 - histone deacetylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIR3344341.1"
                     /translation="MKVFYSDRFTVPLPEGHRFPMPKYSLLRQRAFESGLLHPDEFIE
                     AEPASDIQLLRVHTAEYLEKVKVGLLSEREIRRIGLPWSSQLFERSRRSVGCTIEACR
                     VALQEGLAANLAGGTHHAYPDHGEGFCVFNDVAVAVRAMQAEDHASRMMILDCDVHQG
                     NGTAAIFAGDPTVFTFSIHGEKNFPYHKERSDLDIAMPDGTSDQVYLEALQDGLRRCL
                     EAFQADLAIYLAGADPYIHDRLGRLSLSKTGLVERDRTVLAACHQFGLPVAITMAGGY
                     AKDIHDVADIHMNTLIIAQQFFQNGKQSN"
     gene            complement(4296..4850)
                     /locus_tag="AC16CB_17605"
     CDS             complement(4296..4850)
                     /locus_tag="AC16CB_17605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR3344342.1"
                     /translation="MNVSEAIRMKRAVRQFQEKALPEEAILAILNAGRRSQSSKNTQP
                     WHFIAIRDKATLKALSEYGTWAGHLAGAPLGVAILHSDPSQRFQILFDIGQCAAYMQL
                     AAWEMGIGSCLASIYEPEKARDLLGFPEDLHMRIGISFGYPLDPQDMSRLPQKGGRRS
                     PSEIYHWEKWSLALTKSGRTLVNN"
     gene            complement(4946..7519)
                     /locus_tag="AC16CB_17610"
     CDS             complement(4946..7519)
                     /locus_tag="AC16CB_17610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR3344343.1"
                     /translation="MTLLTNTQATRNAQSEEIHQHEVFTGLTSAEVLKQRDIYGKNVL
                     PSEEKTSAWTIFINQLKSPLVYIILFAATVSLIVGEYGDFGIIMAVVVVDAVLGFFQE
                     YQAKKTYTALKSLIKPTTTVIRDGQRQEVEVHELVPNDLVLLNAGEHVPGDGFLLESA
                     KISVDEAILTGESEPVNKTATSSADVSAESLATDDNRLAMGTTVLTGRGLMRVTGTGS
                     NTELGKIATSLQEHVEEETPLQVRLKVFSKTLTRIVALFTIAILVSGLLLGRGFLDML
                     RTSIILAIAAVPEGLLIAVTVILVLGMRKILKRNGLVKKLLAVETLGSVTTICTDKTG
                     TLTEGRMRVTRTDLADPQRALETMILCNNLEGPVDIALWEYAKDQNQADPQDLVNTSE
                     RLAEELFSSETKYMSTYTRLNGERNDYLKGAPEIVLEMCPISEIDKNQILARVDAWAS
                     EGLRLLGLAYRPHKNLEDHSDYNWVGLLAMEDPIRDEVIEAVRIAHKAGIKIKMITGD
                     YRRTAERIGRSIELLNGDEEILEGGSLAAISDEELSKRVEKISIFARIRPNDKLRIIK
                     ALQANGEITAMIGDGVNDAPALKRANIGVVVGSATDVAKETADLILMDNNFRTIVAAV
                     EEGRIIFENIRKVVAYTLSNSFAEVLTIFIAMMLRWPAPLAVAQILWIHLICDGPSDI
                     VLGFEPKEDGIMDKKPMSIKEPILTPLGMSLIAVISIASTVTALLLFDHFFQMHNNPV
                     EGRSIIFASFAVNSMIYIFAYRSMHQSIFRMRPLIENKPLIWAVAAGLLMAVLPFLVP
                     SLQRLLGIVPLSLSEWALVAGVALGLLLIVELGKWISNYFYNQSESREMRGNQPVGVQ
                     R"
     gene            complement(7554..8021)
                     /locus_tag="AC16CB_17615"
     CDS             complement(7554..8021)
                     /locus_tag="AC16CB_17615"
                     /inference="COORDINATES: protein motif:HMM:NF024212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HIR3344344.1"
                     /translation="MSQPFEEMHFIEDIGLFFEQMGMPRMAGRILGVLLISDPPAQSI
                     TDLSEKLKASKSSISIMARLLVENGLIERVASRVPRRDYYRFKPGGWILYMEQWLGLM
                     AALHQITERGLSLIDEKPPELKERLQEAHDLFSFIEEEFPALLEALQKKRASE"
     gene            complement(8289..>8779)
                     /locus_tag="AC16CB_17620"
     CDS             complement(8289..>8779)
                     /locus_tag="AC16CB_17620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3344345.1"
                     /translation="HILGDMRTIRLSRVYDAVFIHDAISYLTTLSDLEQAIQTAWIHC
                     RTGGVALFAPDFTRETFQAGTSHGGYDAAMRGMRYLEWRWNPDPSDATYVVDFAYLLR
                     DESGDVRVEADRHHMGLFSRLEWLETIHQAGFEASAIPFEHSEIEAGSSEIFVGVKPT
                     ER"
BASE COUNT         2067 a         2293 c         2175 g         2244 t
ORIGIN      
        1 aaatgagctc taactccttg agcttctctt cggtgacaac accgtttctc cagccgcgcg
       61 cttcgtagta ttcttccaac atctcaggca actcgcaaac atgtcccttc gagccagggc
      121 ctgtggaagg ttctttcaga aagcgctcgg gcagggagtc ggagccttct acgaatccta
      181 ttaggttgtt atagtagcgc tccaggttat acacgcgttc gcccgctttg agaagcatgc
      241 cggcatcggc tggcacgcct gtgatagctg tgtattgggc ggcaaacgaa tccaagggtt
      301 cagcgaaggt agcgaacttg caaatatcca aacaatctgt catggtgtgg acattttgaa
      361 agatcactgt gagttgcccc ttgcctttcc actccagcgg gtcggtcaga tctgttgaac
      421 caagtacatt ggcgaccact tcggcagccg gggtgtaacc gcgcagatgg caggcacccc
      481 ggttgctcgt cgcaaatccg atgcccatac ccttgatacc gcgagggtca tacgccggga
      541 ttgccatacc tttgacggtc atggctattt ctggatgccc gaatgtcttc gccgcacgtt
      601 ccgcaccctc agccaggaca ttaccaatac cctcccgtga ggctatctta cggactgttt
      661 ctaccatggc gggatggtct ccccatttca atccacccgc tccattggta tagcctttct
      721 cgctggcttc catgtagacc gagagcacat tacccatctc gatggtatcg aaaccgtaat
      781 cattgcactg gtcaatcaat ttggcgacgc tggcaacatc ggaattgtcg cagttcgcgc
      841 ctaatgccca tgcagactca tattcgacac tttccatctg caagccggcg taaggcccat
      901 ccttgattat tacttctttc ttgcaagcca ccgggcaagc atggcaggtg ggattgttta
      961 ccaggatatt atcgcgtaca tactcgccac tgatcaactc atgatcctca aaataggtca
     1021 gctgggagtt cttggttggc atggcgccga tcacattgac catattcata agcacgtttg
     1081 tgccgagggt cgacaaacca cccttgcgcg gtgatgtgat atacttggga tcctttagtt
     1141 gagccaaggc gtttgcatga gcctctttcc agagctcgcg gtcaaccgct tttggcatct
     1201 tcttggttgc tttgatgacg acagctttga ggttcttgct gcctcccacg cagcctgtgc
     1261 ccccacgacc gctggctcga tcgttttcat tcacccagca tgcaaaccgc gagagtttct
     1321 cgccagcctg accaatggtg atgacactca ggtctttttc tccatactta tccttaaagt
     1381 atttgactgt atcgtgaacg cctttccccc agacatctga agcgtcatga atttcaactt
     1441 taccagcctt gacaaaaaca tatactggtt tttcagcttt tcctttgaaa tttaaaccat
     1501 caaagcctgc ccagcgtaaa cgagcgcctg accagcctcc atggtggctg tcggtgatgg
     1561 tgcccgtaag cggcgacttt gtgacgatgg cgatacgccc actgaggttt acctcagtac
     1621 ccgtcagtgg accattcata aagcttaaaa ggttatcggg agaaagagga tctacctgag
     1681 ggccattgtc aaagacgtac ttcacgccca cacctcgccc cccgatgtac ttaagcttcc
     1741 agtcttcggg gattgcgtcg tacgtgacct gccctttggt taaatcaatg tgagctactt
     1801 tatctgcata ccctccgagt gccatatctg cctccttttg agaatggtga atggaaattc
     1861 tgtgcgataa atcaagctat atcgtaatct atgtcgcctt tgtacctcct tttcaagggg
     1921 cattctgaaa tttattttat ctatttaacc aagttgggat gaaaacttaa atactaaaga
     1981 gagcaatgaa attgctcaaa gtcgaattta tcaatttgga ctttttgaca atagcagaaa
     2041 aataaacttg attcaagtga caaacgtcat gtcacatttc ctcaatgagg aaaaaggttg
     2101 ttccgaaaat aaaaggggtt tgcgtggacg catttcagct gccacacaaa cccaatcctg
     2161 caattaagca aatacacatc tctaaattgg tttggttata tgagatctcc tcaccttatt
     2221 tcgagatact ttcaagatta ttcgctgggg atgatcagcc acagcaagag gtagatcaaa
     2281 attcccggga cgccgccagg gataagggcg atgataaacg caagccggaa ccagaatgcg
     2341 cttatcccga agaactccgc cagcccacca cacacgccca taaaaacacg attgctgcgc
     2401 gaacgacgta atgcttgtcg agtagtcatc atattacctc ctgtaaacct tacatatcca
     2461 tttacgcagg tgaagaataa aagttgcact caggcaacct tgaccgagac cagtgtgccg
     2521 tctccggctg gatagatggt actcatcaag cgtgggttag tggcgatgct gatattaaac
     2581 tcgcgcatag tttcagtgta cgcgtccggt tcagcaagcc cagctacact tccctttctg
     2641 tatgcgttgt gggcggctat gatgcctcct acccgtgtgt gctccaatgc ccaggctaag
     2701 tattttggat aaccagtttt ctccgcatcg ataaagacaa aatcgaatgg tgcctctgac
     2761 gacaattgct ccagggcatg gtgggcgtcg cctaaacgta tttcgatcga ttcgccgacc
     2821 cccgcgaatt cgaagtgctc gcgggctatt tcggcatggc gggattccaa ctcgagcgtg
     2881 atcagtttcc cgccagggac aaggccgcga gcgatccaaa tgccgctgta accacctagc
     2941 gtgccaattt ccagggcttt ttttgcgccg caagcccgcg tcaggaattg caggaaccgc
     3001 ccctcctctg gcttgatgct gatggcaggc agccctttac gaggggtctc atcccaagcg
     3061 cgctggaggc ctttgtcatg gatggcgaac aggttggttg tgtattggct gatctggtcg
     3121 ttatagatgg tcatgtaagg ttactccata atattaattg gattgcttcc cattctgaaa
     3181 gaactgttgg gcaattatga gtgtattcat gtggatatcc gcgacatcat gaatatcctt
     3241 ggcatatccg cctgccatgg tgatcgctac cggcaaccca aattggtggc aggcggcgag
     3301 aaccgtgcgg tcacgctcta caagaccggt tttgctcagg gagagacgcc ccaggcggtc
     3361 atggatgtat gggtccgcgc cggcgaggta aatcgccaaa tctgcctgga aggcttctaa
     3421 acagcgtcgc aacccatctt gcaaggcttc caaatagact tgatcactgg ttccatccgg
     3481 catggcaata tccaggtcgg agcgttcttt gtgatacggg aaatttttct caccatgaat
     3541 ggaaaaggtg aaaaccgtgg gatcgccagc gaagatagca gccgttccgt tgccctggtg
     3601 gacatcgcaa tccaggatca tcatgcggct agcatgatcc tctgcttgca tggcgcgcac
     3661 cgccacggcg acatcgttga aaacgcaaaa gccctcaccg tggtccggat aggcatggtg
     3721 agtgccccct gccaggttgg cagccaggcc ttcctgtaat gctacccggc aagcctcgat
     3781 ggtgcagcct acgctgcgcc gcgagcgctc aaataattgc gaagaccatg gcagaccaat
     3841 tcggcgaatt tccctttcgc tcagcaaccc gactttgact ttttccaggt actcagccgt
     3901 gtgcacccgc aggagttgaa tatctgaggc aggttcggct tcaataaatt cgtctggatg
     3961 cagcaaacct gactcaaacg ctcgttgccg caacaggctg tacttgggca tggggaagcg
     4021 gtgcccctct ggcaagggga cggtaaatcg gtctgaataa aagaccttca taatcgctat
     4081 tgtatcgcga ttaatatggc ggattattta tttcgcaggg caattgcata tgccctgagg
     4141 ggtatgcccc caccaaaaca ccccattttc tctcctcagt ggtgggaaat cgcccaaatg
     4201 cgattgccct atttatttcg ggagttgtgc aaatctggag attatgtctt gctgtctagg
     4261 agacatggtg taagctgctt gttcggaggc acaatttagt tgttaaccaa ggttcgacca
     4321 ctcttggtta aggcgagcga ccatttctcc caatgataaa tttcgcttgg tgaacgcctg
     4381 cccccttttt gtggcagtct gctcatatcc tggggatcaa gtggatagcc aaacgagatg
     4441 ccgatgcgca tgtgtagatc ctccgggaaa ccaagcaagt cacgtgcttt ctctggttcg
     4501 tagatgctcg ccaggcaaga gccgattccc atctcccagg cagctaactg catgtaggcc
     4561 gcgcattgtc cgatatcaaa aaggatttga aagcgctggc tgggatcgga atggagaatc
     4621 gccacaccca gcggcgcgcc agccaggtga ccagcccagg taccgtattc cgagagtgct
     4681 ttgagtgtgg ctttatcgcg tatggcgata aaatgccagg gttgggtgtt cttggatgat
     4741 tgcgagcggc gacccgcgtt cagtatagcc aggatggcct cttcgggcaa agctttctcc
     4801 tgaaactggc gaacagcgcg cttcatgcgg atggcttcgg agacattcat gttttagaac
     4861 ctccaccact attggtctgg aataatcaaa tatatgctga tcagagaaaa gcactcattt
     4921 cagcgctcgt atctcagcaa atcaactatc tttgcactcc aacaggctgg ttaccccgca
     4981 tctccctgga ttcggactgg ttgtagaaat agttgctgat ccacttgccc aattcaacga
     5041 tcagaaggag gccgagtgct acacccgcaa caagcgccca ctcactcagg cttagtggga
     5101 caatacccag caggcgttgc aaggagggca ccaggaaagg cagcactgcc atcaacaggc
     5161 ctgccgccac tgcccagatt aagggcttat tttcgatcag ggggcgcatg cggaatatcg
     5221 actggtgcat gctgcgataa gcaaagatat agatcattga gttgaccgcg aagctggcaa
     5281 agatgatact gcgtccctcc actggattgt tgtgcatctg gaaaaagtga tcgaaaagga
     5341 gcaaagcggt tactgtactc gcaatgctga tcaccgcgat gagggacata cccaacggtg
     5401 tcaagatagg ttctttaatc gacatgggct tcttgtccat aatgccatct tctttgggtt
     5461 cgaagcccag cactatatcc gatggcccat cgcaaatcaa gtggatccaa aggatttgag
     5521 ctaccgccag tggcgccggc cagcgtaaca tcatcgcgat aaagatagtc aacacctcgg
     5581 caaatgagtt ggaaagggtg taggctacta ctttgcgaat attctcgaaa attatcctgc
     5641 cttcttccac ggccgctaca atagtgcgga agttgttgtc catcaggatc agatcagcgg
     5701 tttccttggc gacatccgtc gcgctgccca ccacgacacc aatgttcgcc cgtttgagtg
     5761 ctggggcatc gttgacccca tcgccgatca tagcggtgat ctcgccattg gcttgcaagg
     5821 ctttgataat tcgcaattta tcattgggcc gtatgcgggc gaagatagat atcttttcta
     5881 cccgcttaga gagttcctcg tcggagattg cggctaagct cccaccttcg aggatttctt
     5941 catccccgtt caacaactca atacttcgcc cgatgcgctc agctgtgcga cggtaatcgc
     6001 cggtgatcat cttgattttg atgcccgctt tatgcgcgat gcggacagct tcaatgacct
     6061 catcccgaat tgggtcttcc attgccagca gccccaccca gttatagtcg ctatgatctt
     6121 ctaagttttt atgaggtcgg tacgctagac cgagcaggcg cagcccttca ctggcccacg
     6181 cgtcgactcg ggccaatatt tggtttttat cgatctccga aatggggcac atttccagca
     6241 caatctctgg ggcccccttt agatagtcgt tgcgctcacc gttcaagcga gtgtacgtgc
     6301 tcatgtactt tgtttcgctg ctgaaaagct cttctgccag ccgttcagat gtgttcacaa
     6361 gatcttgtgg atcggcctga ttttgatctt ttgcgtactc ccacaatgca atatcaactg
     6421 gaccttccag gttgttgcac aggatcattg tctcgagagc gcgctgggga tcggctaaat
     6481 cagttcgagt tacgcgcatg cgtccttctg tcagcgtgcc agtcttgtca gtgcaaattg
     6541 tggtcactga ccccagggtt tcaactgcaa gtaatttctt gaccaggcca ttgcgtttga
     6601 gaattttacg catgcccagc accagaatga ctgtgacagc gatgagcagg ccttcgggaa
     6661 ctgcggcgat agccaggata atcgatgtgc gcagcatatc cagaaagccg cgcccgagca
     6721 ataagcccga gaccaagata gcaatcgtga agagagccac gatgcgcgtc agtgttttac
     6781 tgaatacctt taaccgcacc tgtagtggtg tttcctcttc tacgtgctcc tggaggctgg
     6841 tcgcaatctt ccctaactcg gtgtttgaac ctgtgccagt cacgcgcatc aatccacggc
     6901 cagtcaaaac cgtagtcccc atagctaagc ggttgtcatc cgtagctagc gattcagcag
     6961 aaacatccgc agatgaggtg gctgtcttat tcaccggttc gctctcgccg gttaatattg
     7021 cttcgtcaac ggatattttt gcgctctcca ggagaaaccc atcgcctggg acgtgctcgc
     7081 ctgcgttgag gagaaccagg tcgtttggta ccaactcgtg cacttccact tcttggcgtt
     7141 gcccgtcacg gattacggta gtggtgggtt tgatcaagct ttttaaagcg gtataggttt
     7201 tctttgcctg atattcctgg aaaaacccga gaacggcatc cacaacgacg acagccatga
     7261 tgataccgaa atcgccgtac tcaccgacga tgagggagac cgttgctgca aataatatga
     7321 tatataccag cgggctcttg agctggttta taaaaatcgt ccacgctgag gttttctcct
     7381 cagagggcag aacgtttttg ccatagatgt ccctctgttt aagcacctcg gcgctggtca
     7441 ggccggtaaa tacttcgtgc tggtggattt cttcagattg cgcgtttcta gtcgcttggg
     7501 tattggtgag caaagtcatg atgatcccct cgattattgt gatgaaagca tgattattct
     7561 gaggcgcgct ttttttgtaa tgcttcgagc agcgcaggga attcttcctc gatgaaagaa
     7621 aacaagtcgt gggcttcttg taaacgttcc ttcaattctg gaggtttttc atctatcaaa
     7681 gataagcctc gttcggtgat ctgatgcaag gccgccatta accccagcca ctgttccata
     7741 tacaaaatcc agccaccggg cttaaagcgg tagtaatcgc ggcgcggtac acgcgaagcg
     7801 acgcgctcga tcaggccgtt ttccaccagc aagcgcgcca taatgctgat ggagctcttg
     7861 ctggctttta atttttcgga caggtctgtg atggactgtg ctggtggatc tgagatcagc
     7921 aacacgccta agatgcgccc agccatgcgc ggcatgccca tctgttcaaa gaacagacca
     7981 atatcctcga taaaatgcat ttcttcaaaa ggttggctca tagtcaatac tcgcagagtg
     8041 agatgatgga attaagtata aaactttaga aacgttttgt aaatactaaa cgttacaaac
     8101 aaaacggcaa ttaaacggtt gtccattact acaaatgagc agccggatat gaatttattg
     8161 cttcgagata ttagatggaa aaatatttat cgggggacta atctaattgc tcgttctatc
     8221 atattcccta gcgagtggtt gctgaagggc ggcgatctcg attgtgctac gagtgcatca
     8281 tagctgggtt atcgttcagt tggcttgacc cccacgaata tctcgctgct accggcttcg
     8341 atttcactgt gttcaaaggg tatcgcgctt gcctcaaaac cagcctgatg gatcgtctcc
     8401 agccactcca atcggctaaa aagtcccata tggtggcggt ccgcctcaac ccgcacatcg
     8461 cctgattcat cccgtaatag gtaggcgaaa tccaccacat aggttgcatc agacgggtct
     8521 ggattccacc tccactcaag atatcgcatc cccctcatgg cggcgtcata gccgccgtgg
     8581 cttgtgcctg cttgaaacgt ctctcgagtg aaatcaggtg cgaagagcgc cacacctcca
     8641 gtccggcagt ggatccatgc agtttggata gcctgttcga ggtcgctgag cgtggtgaga
     8701 taactgatcg cgtcatggat gaagaccgca tcgtagacgc ggctgagcct gattgtccgc
     8761 atgtcaccca ggatatgct
//
LOCUS       DBNGSX010000609         9928 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_344028, whole genome shotgun sequence.
ACCESSION   DBNGSX010000609 DBNGSX010000000
VERSION     DBNGSX010000609.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9928)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9928)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9928
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_344028"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      319..464
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            680..1489
                     /locus_tag="AC16CB_17625"
     CDS             680..1489
                     /locus_tag="AC16CB_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3344346.1"
                     /translation="MDITRTEKTLPWPVRNLGRSRIYLALFSMLLTLLLWGAVTSLTD
                     FPAFILPGPWLVWARFWQLVIDGSLLRHILVTLQEVLLGLALGVSAATVLGYLLAKSP
                     VFERLLSPYIVASQSVPVVAIAPLLIIWFGPGMFSKILICALIVFFPVLVNTIVGLRS
                     VPEELEDLMRSLQATRWQMAAYLQVPAALPVFLGGLRIGATLSVIGAVVGEFVGADRG
                     LGFLINLGRGQYDTALVFVAVFTLVTLALGMYGAVVFIEYRWLSWQQRIEL"
     gene            1524..2537
                     /locus_tag="AC16CB_17630"
     CDS             1524..2537
                     /locus_tag="AC16CB_17630"
                     /inference="COORDINATES: protein motif:HMM:NF020650.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3344347.1"
                     /translation="MAHKTILTVALVFLLALAACSPSATPSQEITESPQETEPPLAHI
                     RLPLGYIPNIQFAPLYVAVEKGYYQAEGIEIEFDYSFETDGVSLVGAGELQFAVVSGE
                     QVLLARAQELPVVYVMAWYQDYPVGVVAKTSQSIRTPQDLAGKKIGLPGPFGASYIGL
                     RALLNAGGLQESQVTLDSIGFNQVEALAADQEQAVVIYIANEPVQLRAKGYEVDEIRV
                     SDYVRLASNGLITNEATIQQNPDLVRAMVRATLRGIADVIANPDEAYKISLEYVEELA
                     QADEAIQKEILATSITFWQADQLGVSDISAWENMQAVLLDMGLLAQPLDLSKAFTNEF
                     ISR"
     gene            2518..3285
                     /locus_tag="AC16CB_17635"
     CDS             2518..3285
                     /locus_tag="AC16CB_17635"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3344348.1"
                     /translation="MNSSADKPIVAINHLSAIFDNGNGGLRALEDISFSVSSQEFVSV
                     LGPSGSGKSTLLRILAGLLPPTRGQVFYQGEELSGPRRGVGFVFQRANLMPWRTVLEN
                     ITLPLELLGTPHQTTLERAQELVNLVGLQGFEDSLPRDLSGGMAQRVAIARALIHDPD
                     VLLLDEPFGNLDALTRERMGAELLRIWQARRKTVIMVTHSISEALFLADRVLVLTPRP
                     GRLRLDLSVELPRPREDDVRYTPVFGKLVRQVREAIG"
     gene            3408..4337
                     /locus_tag="AC16CB_17640"
     CDS             3408..4337
                     /locus_tag="AC16CB_17640"
                     /inference="COORDINATES: protein motif:HMM:NF024637.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HIR3344349.1"
                     /translation="MMHKYSLVILVIMIIFAAYAPLTPAFAQDEIKLSNLEVDLWPEY
                     DRPSVLVIYRITLPPTISLPVDLTFRIPAAAGDPSAVALRQMTPEGDSGLFTIPYQRQ
                     VVGEWGMITITATVPEIQLEYYDPDLVKQDDARQFEYRWPGDYAIDALILQVQQPLGA
                     SEMVISPASDPGVSGNDGLVYYNKQVGSLAAGQTFSLTVNYNKASDDLTAANLQVQPS
                     APVSVTPGQNSLMTLLPWALGILGVLLILGGVVWYRQSGRGKPGDRPRRRRATASREP
                     EESTSFIYCHQCGKRASPGDRFCRTCGTKLRTE"
     gene            complement(4556..6733)
                     /locus_tag="AC16CB_17645"
     CDS             complement(4556..6733)
                     /locus_tag="AC16CB_17645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344350.1"
                     /translation="MKSKYLLMGVALLALAMVIAACAQAPAPVETTPCPEAPPCPDCP
                     APPEPPSCPEAVVKDVPFEAEWVGSPHNDTEAEAFNHWNEDDPQEVPTSCARCHSTPG
                     YQDYLGADGSAVGEVDQAAPIGTTIQCVACHNAATATLSSVTFPSGVEVTGLGREARC
                     MVCHQGRASKVQVDSSLEQAGLLEDLDTVNAELGFINIHYYAAGATLYGGVTMGGYQY
                     EGKMYDAKFRHVEGIDTCVGCHNPHTTELKLDVCAGCHEGVAAKEDLKKVRMNGSLAD
                     YDGDGDTSEGVAEEMDGLRAMLLTAIQAYASEVAGAPIGYNSAAYPYFFIDTNADGEI
                     SDDEAVSDNRYVSWTGRLLRAGYNYQVSVKDPGAHAHGGKYIIQLLYDSIEDLNTALS
                     SPVDLSTAHREDPGHFAGDTEPFRHWDEEGEVPGSCARCHSATGLRTYIQEGVNVAAE
                     IANGFMCVTCHNEEEWPARYAVNEVTFPSGATLTFGEGKESNLCIVCHQGRSSTPTVN
                     RALGDKPADTVDDTIRFSNIHYFAAGATIFGNDAQGAYVFDGKEYVGPHPHVAQAGFE
                     CKSCHDVHALDVKVETCAGCHGGSSDPTDPDTYRIDATDWDGDGDTTEGVKSEIASFG
                     ERLYAGLQAYAKDKGTPLVYDSHSYPYFFVDADENGEPDANAEGGLVGYNAWTPNLLK
                     SAYNYQYWQKDPGNFAHNAKFMLQILYDSIENVGGDVAGLTRP"
     gene            complement(6886..9039)
                     /locus_tag="AC16CB_17650"
     CDS             complement(6886..9039)
                     /locus_tag="AC16CB_17650"
                     /inference="COORDINATES: protein motif:HMM:NF012262.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3344351.1"
                     /translation="MEEKEKQQYYEKYRKAKEKGIFFYPDAIFKDAVVVFVIFLILVG
                     LAYFIGAPLEERANPADTSYTPRPEWYFLFLFQLLKYFPGELEVLGVVVLPTLAIILL
                     FALPFLDRSSRRHFRSRPLFVGVTALMMIGVVALTVQSYREAPPPAEEIAGDQTAALY
                     AKNCAGCHGPSIDVAAGTNLHAIIAQGKHEGMPAWSADLTSDQIDALAGFVLSPGGSK
                     LFTANCGECHSAADMVAGDPIELKKAIEQGKEYPVHADVDIPPWNEVLSQGERTALLN
                     FLVAPDGQRLFATNCSPCHGRSVAVSGGESELRTLISQGGLHLEMPPWREQLSDTELD
                     SLARYVVDPGSAPESEGLFGTYCASCHGERVPTSEDVDQAREIIASGGSHETMPVWGA
                     VLTPEQLDALVSYTLSAAKGTSLDVGQELFSTNCAACHGDFGEGGANPTRSNDIIAPI
                     STSEYLKTRDDFTLRAIIAQGQPNFGMSPFSSSFGGPLDDEEIDAIVAYMRSWEENPP
                     VELPPEVVPTQVSISETDIYANLCAQCHGPNGEGGLGPSISDPEFQAAYTDQEIFDAI
                     NRGHKATSMIGWGEVLSADQIQKLVGFIRKLQPAAEQGPAPGASTVPSATPSPGAAPA
                     AVSFSQDVLPIFEAKCTMCHGVLGGWDGTSYEAVINTGNNAPVVIPGDPENSLLAQKL
                     LGTQTEGAIMPPAGKLPDAEIQIILDWIAAGALEE"
     gene            complement(9043..9702)
                     /locus_tag="AC16CB_17655"
     CDS             complement(9043..9702)
                     /locus_tag="AC16CB_17655"
                     /inference="COORDINATES: protein motif:HMM:NF012263.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - quinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HIR3344352.1"
                     /translation="MATKIGNWLDERLAWRQVWTAIFLRKIPKVNWLFTLGSASLFLA
                     VNQIVTGILLTIYYVPTPDHAYDSVQYITTQVPMGWLIRGLHHWGASAMVVVVVLHML
                     RIIFYGAYKFPREVTWFTGVILLIVTIGFGFTGYLLPWDQKAYWATTVGTRIAGTPPV
                     IGEWILLIIRGGEELSAVTLARFYGVHIWVLPTVLLLITLIHLYLVIRIGISAVPGRE
                     E"
     gene            complement(9704..>9928)
                     /locus_tag="AC16CB_17660"
     CDS             complement(9704..>9928)
                     /locus_tag="AC16CB_17660"
                     /EC_number="7.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="QcrA and Rieske domain-containing protein"
                     /protein_id="HIR3344353.1"
                     /translation="YVLTDNGRDFIAMSNICSHLGCRVRWISDQEIFFCPCHNGVFDK
                     EGAVEAGPPPGPLDRYELKVEDDQLFILGG"
BASE COUNT         2238 a         2814 c         2629 g         2247 t
ORIGIN      
        1 cttttccgag atattggtca ggcgtggcga ttgtgcctcc agcaacgccc ggatgatctt
       61 cgatccttgc tcagctagtt tttcgtcatc caccaggtag cgcataaatt caccaaagtt
      121 gatacacttg tccatggaag cctcctttga gtttcccgtt catagcggtt actcaattta
      181 gaggcttctt cctttttttg caatactttg tccgaacttt cgtccgaagg tcacgattga
      241 cggattgaat tgcaggggcg aataggttat aattgaaatc aacaaacgat gtgagttatc
      301 aatcacatcg tccataacca aaaccttcgg ggcagggtga aaggcgtatg aagcgcctca
      361 ttcccgaccg gcggtaaagc ccgcgagcgc ccggcagata gacgggtgca cgatccggtg
      421 aaactccgga gccgacggta tagtccggat gaaagaaggt agcgctgttc acgttgccag
      481 ggcaagcgcc ccagctagtt gagcagtcaa tgctagccca gagcgggacc atctccgcgc
      541 cacatgcccc gaaaaccatc cggttttcgg gtttctcttt acagcaaggt gtgccgcaga
      601 ttttgagcat ccgcgaaggc tgtactgcgc ctgccccgga gtccctaaac tctggggcgc
      661 tttatttgac aggatgaata tggatatcac gcgcacagaa aaaacacttc cttggccggt
      721 tcgcaatttg ggacgctcca ggatatatct ggcactattt tccatgctgc ttacgctcct
      781 gttatggggt gctgtaacga gcctgacgga ttttccagcc ttcatactcc ctggcccgtg
      841 gctggtctgg gcgcgcttct ggcagcttgt cattgatggc agcctgctgc ggcatatttt
      901 ggtgacattg caagaagtcc ttttagggct ggcgctgggt gtgagcgctg ctacagtctt
      961 aggctacctg ttggcaaaat cgccggtatt tgagcgactg ctctcgccat acatcgtcgc
     1021 cagccaatct gtgcctgttg tggctatcgc cccactgctc atcatctggt ttggaccagg
     1081 catgttctca aaaatactga tctgcgccct gatcgtcttt ttccccgtgc tggtcaatac
     1141 catcgtcgga ctgcgctctg taccggagga gctggaagat ctgatgcgct cgctgcaagc
     1201 cacccgctgg caaatggccg cctacctgca agtgcctgcc gccttgccag tcttcctggg
     1261 aggcctgcgc atcggggcca ctctctcggt tatcggcgca gtagtgggtg agttcgtcgg
     1321 cgcagaccgc ggtttgggct ttttgatcaa ccttgggcgc ggccagtacg ataccgccct
     1381 ggtattcgtg gcagtcttca cgctggttac actggcgctg ggcatgtacg gcgccgtcgt
     1441 ttttatcgaa taccgctggc tttcctggca acaacgcata gagctgtagg atcagccaga
     1501 tagattttgg gaggattttt tggatggcac acaaaacgat actcactgtt gctctcgtct
     1561 tcctactagc gctggcagct tgctcgccga gcgcgacccc atcacaagag ataaccgaat
     1621 caccgcagga gaccgagcca cctctggcgc acattcgcct accactgggc tacatcccca
     1681 atatccagtt cgcgcccctg tacgtcgctg tagaaaaggg ctattaccag gctgagggca
     1741 tagagataga gttcgattac agctttgaga ccgacggcgt ctccctggtg ggggcaggcg
     1801 agctacaatt tgccgtggtc tccggcgagc aagtgctgtt ggcgcgcgcc caggagctac
     1861 cagtcgtcta tgtgatggcc tggtaccagg attacccggt gggtgtggtg gcaaaaacca
     1921 gccagagtat ccgcactccg caggatctgg cgggaaagaa gatcggccta ccaggaccat
     1981 tcggagccag ctatatcggc ttgcgcgcct tgctcaatgc aggcgggttg caagagagcc
     2041 aggtaacttt ggattcgatc ggcttcaacc aggtcgaagc gctggccgca gaccaggagc
     2101 aggcagtggt catttatatc gccaatgagc cggttcaatt gcgcgccaaa ggatatgaag
     2161 tggatgaaat ccgcgtctcc gattacgtcc gcctggcatc caacgggcta atcaccaacg
     2221 aagccaccat ccagcaaaat ccagacctgg tgcgcgccat ggtgagagca acactgcgcg
     2281 gtattgctga tgtcatagcc aatccagacg aggcgtacaa aatcagcctc gaatacgtag
     2341 aagagctggc tcaggcagac gaggcgatcc aaaaagagat cctcgccaca tccatcacat
     2401 tctggcaagc cgatcaattg ggcgtatcgg acatttccgc ttgggagaac atgcaagctg
     2461 ttctgctgga tatggggctg ctcgcccaac cgctggattt gagcaaggct ttcacgaatg
     2521 aattcatcag ccgataaacc catcgtcgcc atcaaccacc tgagcgccat ttttgacaat
     2581 ggcaacggag gcttgcgagc gctggaggat atctccttct cagtgagctc acaggagttt
     2641 gtcagcgtcc tcggtccgtc tggcagcggc aagagcaccc tgcttcgcat actcgcaggt
     2701 ttattgcctc ccacccgagg ccaggttttt tatcagggtg aagagttaag cggaccacgg
     2761 cgcggggtcg gattcgtgtt ccagcgcgcc aacctgatgc cctggcgtac ggtattggaa
     2821 aatattactc ttccgctgga gctgctgggc acaccccacc agacaaccct ggaacgcgcc
     2881 caggagctcg tcaatctcgt cggattgcag ggtttcgagg acagcctgcc gcgcgacctc
     2941 tccgggggta tggcgcagcg tgtcgccata gcccgcgccc tgattcacga tccagatgtg
     3001 ctgctgctag atgaaccatt cggcaacctg gatgccttga cgcgcgagcg catgggagct
     3061 gagctgctgc gcatctggca ggcaaggcgc aagaccgtga taatggtcac gcactcgatc
     3121 tctgaggcgc ttttcctggc cgaccgtgtc ctggtactca cacctcgccc cgggcgcttg
     3181 cgcctagact taagcgttga actgccccga cctcgcgagg acgatgtgcg ctacactccg
     3241 gtcttcggca agctggtgcg gcaggtacgc gaagcaattg ggtagtccct aacttcctgg
     3301 gaatctacct cggaaatggg tataagtccc ctttccgcta actctgccct ccccgtatgc
     3361 ctcattgcat gataagattc ccaaccgaat ttcattacga ggaagaaatg atgcataaat
     3421 attcccttgt tattctggtg atcatgatca tcttcgccgc ctacgcgccg ctcacgccag
     3481 ccttcgctca agatgagatc aagctgagca atctagaagt ggacctgtgg cctgaatacg
     3541 atcgtccatc tgtcttagtc atctatcgca tcacgctgcc ccccactata tctctgccag
     3601 ttgacctgac atttcgcatc ccggccgccg cgggtgaccc gagtgccgtg gcattgcgcc
     3661 agatgactcc cgagggcgat tcagggttat tcacaatccc ttaccaacgc caggtcgttg
     3721 gggagtgggg catgataacc atcacagcca ctgtacccga gatccagctc gagtattacg
     3781 acccggattt agttaaacag gatgacgctc gccagttcga gtatcgctgg ccgggagatt
     3841 acgccattga tgcgctaatt ttacaagtcc aacaaccgct gggtgccagc gaaatggtca
     3901 tctcgcctgc atcagacccg ggtgtgtccg gcaatgatgg gctggtgtat tacaacaagc
     3961 aggttggctc actggctgct gggcaaacct tctcgctgac agtcaactac aacaaagcct
     4021 ctgatgatct gactgccgct aatcttcagg tgcagcccag cgcgcctgtc agtgtcacgc
     4081 ctggtcaaaa cagcttgatg accctcttac cctgggctct gggcatcctg ggggtactgc
     4141 tgatccttgg cggtgtagtt tggtaccggc agtcaggcag aggcaaacca ggcgatcgcc
     4201 ctcgacgacg ccgggcgaca gcctcgagag aaccagaaga atcaaccagt ttcatctact
     4261 gccaccaatg tggtaaaaga gcctcgcctg gagaccgctt ctgccgcacg tgcggcacaa
     4321 aattaagaac cgagtaaccc tcaaaaaaca tataacaccc cgaataataa atttcgggta
     4381 tttcgcctga ttgacggaaa aacgggttat tgggtggcgg taccgccgcc caataacccg
     4441 tttttccgtt atttcccccg ccctcccgcc gggagggcgg ggttaggggt gggtgggttg
     4501 ataaaaatca tgactcaacc cgaaaaagtt attcgggatg agtcactttt atggactaag
     4561 gtcttgtcaa gccagcgaca tcaccaccaa cgttctcgat gctgtcatac aagatctgca
     4621 gcatgaactt ggcattgtgg gcgaagttgc ccggatcctt ctgccagtac tggtagttgt
     4681 aggcagactt gagcaagttg ggcgtccagg cattgtagcc taccaggcct ccctcggcat
     4741 ttgcgtccgg ctcaccgttc tcgtctgcat cgacgaagaa gtacggatag ctgtgggagt
     4801 cgtaaacgag cggggtgccc ttatccttgg cataggcctg gagaccagca tacagacgct
     4861 caccgaagct agcgatctca ctctttacac cctcggtggt atcgccatcg ccgtcccagt
     4921 ccgttgcgtc aatgcggtag gtgtcgggat ctgtcgggtc tgatgacccg ccatggcagc
     4981 cggcgcaagt ttcaaccttg acatccaaag catggacgtc atggcaggat ttgcactcaa
     5041 agccagcctg tgccacatgg ggatgcggcc caacgtattc tttgccgtca aacacgtacg
     5101 cgccctgggc atcattgcca aagattgtag cgcccgccgc gaagtagtgg atgttggaga
     5161 atctgatcgt gtcatccacc gtgtcggcag gtttatcccc aagcgcccga ttgaccgtgg
     5221 gagtagagga gcgtccctgg tggcatacaa tgcacaggtt ggactccttg ccctcgccga
     5281 aggtcagcgt ggcgccgctg gggaaggtga cctcattgac tgcatagcgg gcaggccatt
     5341 cttcctcgtt gtggcaggtg acgcacatga aaccgttggc tatttcggcc gcgacattca
     5401 caccttcttg aatgtaggta cgcaagccgg tagcgctgtg gcaccgtgca caacttccag
     5461 gtacctcacc ctcttcatcc caatggcgga atggctcggt gtcgccggca aagtgaccgg
     5521 gatcctcacg gtgtgctgta gacaggtcca ccggtgagct cagggcagtg ttcaggtcct
     5581 cgatcgagtc gtagagcagt tggatgatgt acttaccgcc atgagcgtgc gcaccgggat
     5641 ccttcaccga cacttggtag ttgtaaccag ctcgcagcaa acgaccggtc caggagacgt
     5701 atctattgtc agatacagct tcgtcgtcgc tgatctcgcc gtctgcgttg gtgtcaatga
     5761 agaagtacgg gtaagccgcc gagttgtagc caatgggagc accagctacc tcgctggcat
     5821 acgcctgaat agcggtcaac agcatggcgc gcagcccgtc catctcttct gcaacgcctt
     5881 cgctggtatc accatcgcca tcgtagtcag ccagagaacc attcatgcgc actttcttca
     5941 ggtcctcctt agcagctacg ccctcatggc atccagcgca cacatccagc tttagctcag
     6001 tggtgtgtgg gttatggcaa cctacgcaag tgtcgatacc ttccacatgg cggaacttgg
     6061 catcgtacat cttgccttcg tattggtagc cgcccatagt cacaccgccg tacagagtgg
     6121 caccagccgc gtagtagtgg atattgatga agcccagttc ggcgttcact gtatccaggt
     6181 cttcaagcaa gccagcttgc tccagggaag aatccacctg gactttggaa gcgcgaccct
     6241 gatggcagac catgcagcgc gcttcacgcc ccagcccggt gacttccacg ccagatggga
     6301 aagtcacgct ggacagagtc gcggtagccg cgttgtggca agccacacac tgaatagtcg
     6361 tgccgattgg ggctgcttga tccacctcgc ctaccgcaga tccatcagcg cccaggtaat
     6421 cctggtagcc aggcgtgctg tggcatcttg cacaggaggt ggggacttcc tgagggtcgt
     6481 cctcattcca gtgattgaag gcctctgcct ctgtatcatt gtgtggcgag ccaacccact
     6541 cggcttcgaa tgggacatcc ttcaccacag cctccgggca agatggaggt tctggtggag
     6601 ccgggcaatc cggacaaggc ggcgcctctg gacaaggcgt cgtctcaacc ggagcaggtg
     6661 cttgggcgca ggctgctatg accattgcca gggctagcag cgcaactccc ataagcagat
     6721 acttggattt catatacata ctcctctctg atgatgaaac tcattggtca ggacgtattc
     6781 tttatgtatt tttctcttgt ctcacctcct tgattctcca aagtcttatt actcaagtag
     6841 tgggcgtaac gaccctgatg ggtgacaagc tacatgaagg gtcgactact cctccagggc
     6901 gccagccgca atccagtcca ggatgatctg gatttcggcg tcaggcaatt tccctgcggg
     6961 tggcatgatg gcaccttcgg tttgtgtgcc gagcagcttc tgcgccaaca ggctattttc
     7021 aggatcgccc ggtataacta ccggagcgtt attcccggtg tttatcacag cctcgtagct
     7081 ggtaccatcc caaccaccta aaacaccgtg acacatggtg catttcgcct caaaaatggg
     7141 caaaacatcc tgggagaaag atactgcagc cggagccgcg ccagggcttg gggttgcact
     7201 tgggactgtg gatgcacctg gtgcaggtcc ctgctcagca gcgggttgca acttccggat
     7261 aaagcctacc agtttctgaa tttgatctgc actcagtacc tcgccccatc cgatcatgga
     7321 tgtggctttg tgacctctat tgatggcatc gaaaatttcc tgatctgtgt atgcagcctg
     7381 gaattccgga tcactgatag aaggtccaag cccaccctct ccatttggac catggcattg
     7441 cgcacacaga ttagcataga tatctgtttc actgatcgat acttgggttg ggacaacttc
     7501 agggggaagc tccaccggcg ggttctcttc ccaagagcgc atatacgcca cgatggcatc
     7561 gatctcttcg tcatccagcg gcccaccgaa actgctactg aagggcgaca tgccaaaatt
     7621 gggctgtccc tgggcgatga ttgcccgcag tgtgaagtca tcacgcgtct tcaagtattc
     7681 gctggtgcta atcggggcaa tgatgtcatt cgagcgggtt ggattggcac ccccttcgcc
     7741 aaagtcgcca tggcaagctg cacagtttgt ggagaataat tcctgcccca catccaagga
     7801 tgtcccttta gcggcagaca gggtgtagct taccagggca tccagctgct caggggttaa
     7861 tacagccccc caaaccggca tggtctcatg agagccgccg ctggcgataa tctcacgcgc
     7921 ctgatcaaca tcttcgcttg taggaacacg ctctccgtgg caggaagcac aataagtgcc
     7981 gaacaatccc tccgattcag gtgccgagcc tggatcgacc acgtaccttg ccagactgtc
     8041 taactcggta tcactcaatt gctcccgcca aggtggcatc tccagatgca gcccgccctg
     8101 gctgatcaag gtgcgcagtt cgctttctcc acccgaaact gcaaccgagc gaccatgaca
     8161 gggagagcag ttggtcgcaa atagccgctg accatctggt gcaaccagga agttaagcaa
     8221 ggctgttcgc tcgccctggc tcaagacctc attccatggg ggaatatcca catccgcgtg
     8281 aactgggtat tctttacctt gctcgatggc ctttttaagt tcgattggat cgcccgcaac
     8341 catgtccgct gcgctatggc attcaccgca gttcgccgta aagagcttgc tcccccccgg
     8401 agagaggacg aagccagcca gggcatcgat ttgatccgag gtgagatccg cactccaggc
     8461 aggcattcct tcatgcttgc cttgtgcaat aatggcatgc aggtttgtgc ccgctgcaac
     8521 atcaatagat ggcccgtgac aaccagcgca gtttttagcg tagagcgcag ccgtttgatc
     8581 accagcaatc tcttctgccg gaggtggggc ttctcgatag gactggactg tcaaagcaac
     8641 aaccccgatc atcatcaaag ccgtcacacc cacgaacaat ggccggctac gaaaatgccg
     8701 cctggatgac cggtctagaa atggcagcgc aaataacagt atgattgcca gggtaggcaa
     8761 taccaccaca cccaagacct cgagttcgcc cggaaagtat ttcagcagct ggaacaagaa
     8821 caggaaatac cactctgggc ggggcgtgta ggaggtatcg gctggatttg cgcgctcttc
     8881 cagcggcgct ccaataaaat aggccagccc gacgaggatc aggaaaatta caaagacaac
     8941 gactgcatct ttgaagatgg catccggata aaagaatata cctttttcct tggcctttcg
     9001 atacttttcg taatattgtt gtttttcttt ttcttccatt acttattcct cgcgcccagg
     9061 gacagcactg ataccgatac ggatcaccag gtataaatgg atcaaggtga tcaacaacag
     9121 aacggtaggc aaaacccaga tatgcacacc atagaaacgc gctaaagtaa ctgcagagag
     9181 ctcctcacca ccgcgaatga tcagcaatat ccactcacca atcactggcg gggtccctgc
     9241 aatccgcgtg cctacggttg tagcccagta tgctttctga tcccaaggca gtaggtaacc
     9301 ggtaaaacca aacccaatcg tcacaattag taaaatcacg cccgtgaacc aggtcacctc
     9361 tctagggaat ttataggcgc catagaagat aatgcgcagc atgtgcagta caaccacaac
     9421 caccatggca cttgcacccc aatggtgtaa gccgcggatt aaccatccca tcggaacctg
     9481 ggtggtaatg tactgaacac tatcataggc atgatcaggg gtgggcacat aataaatcgt
     9541 caacaatata cccgttacga tctggttgac agccaagaac agggaagcgc tgccaagcgt
     9601 aaaaagccaa tttactttcg gaatcttccg aaggaatatg gctgtccaaa cttgacgcca
     9661 agccagacgc tcatcgagcc aattcccaat ttttgtagcc aacttaacct cctaaaatga
     9721 acaactgatc gtcttctacc tttaattcgt accgatcaag tggaccgggc ggaggacctg
     9781 cttccaccgc tccttctttg tcaaaaactc cgttatgaca agggcagaag aagatctctt
     9841 gatcagagat ccaacgcacc cgacacccaa ggtgagaaca gatattggac atggcgataa
     9901 aatcgcgccc gttgtcggtt aacacgta
//
LOCUS       DBNGSX010000610         2711 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_413765, whole genome shotgun sequence.
ACCESSION   DBNGSX010000610 DBNGSX010000000
VERSION     DBNGSX010000610.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_413765"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1439
                     /locus_tag="AC16CB_17665"
     CDS             <1..1439
                     /locus_tag="AC16CB_17665"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3344354.1"
                     /translation="NWDDNHGHGTHVTGSIGGSGVQSGANPAQHLYSGSFAGIAPEAG
                     LVIQAFEATPAGEIIGLDPDYYTLFQQAYEDGARLHSDSWGDLTGPISDTASAYGGYP
                     FGAARTDAFLWDHPDMAIFIAAGNSGKDGTPGALGFCFGGDGVVDPDSLLSPGTAKNV
                     ITVGATESDRSIGGYGGMPWLLLSLCFTAQPIAIDTIANNSAGMAAFSSRGPSDDGRI
                     KPDIVAPGTNIVSNVSHVPGSSSLWGFFENNPDYAYSGGTSMATPLAAGAGVLVRQWL
                     VQKGLSNPSAAAVKATLLNTTADIAPGQYGTVEFQEIPHSRPNYVEGWGRVNLDFTIS
                     DPPNLFWLDDRLVGLSTGQAVTYNSTITQPLEILTDTLPLRVMLAWTDPPASLSATSQ
                     LVNDLDLTVTGPDGTVYHANDNATFDRTNNVEGVVIDKPPLGEYSITVHGFNVPISSQ
                     PYALAVWGPLQPDISSFFQSVYLPVLMK"
     gene            complement(1563..2288)
                     /locus_tag="AC16CB_17670"
     CDS             complement(1563..2288)
                     /locus_tag="AC16CB_17670"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3344355.1"
                     /translation="MSSNYSPVVDDVISVIRHLGQQSAALELTKSFRGIIFKQDVSIL
                     EVSPDDATFRASNIEMCAATETEVYLHNQLFPKPVMAQLKSLNIRKGMLVLSDFAYAD
                     MEWKKRQHERVQPKCPIYVTLHWKGKALRACMENISVDGLGLLAYKLFEKGMNIRPGS
                     NIRLDFQLSPGQKFSALRGRIIYINTIDRYSTTMGIRLFPKVKEVRVLEKYVARRKQE
                     ILEELNQEYSELSKPRGVDSLYF"
BASE COUNT          652 a          685 c          692 g          682 t
ORIGIN      
        1 agaactggga tgataatcac gggcatggca cccacgtgac tggctcgatt ggtggctcag
       61 gagtgcaatc cggagcgaat ccagcgcaac atctctatag cggttctttt gcagggattg
      121 cgccagaagc cggtctggtg atccaagcat ttgaagctac gcctgctggc gagatcatag
      181 gtctggaccc agattactat actctcttcc aacaagccta tgaagatggg gcgcggctgc
      241 actccgatag ttggggagac ctgactggac caatcagcga tactgccagc gcgtatggcg
      301 gttatccctt tggcgcggcg cgcaccgatg cgttcctctg ggatcatcct gatatggcaa
      361 tcttcatcgc tgccggcaac tcgggcaagg atggcacgcc gggagcattg ggattctgct
      421 ttggagggga tggcgtggtc gatcccgact cgctgctctc acctggcacg gcgaagaacg
      481 tgatcaccgt aggggcgacg gagagcgatc gcagcatcgg cggctacggt gggatgccct
      541 ggctgctgct cagcttatgc ttcaccgccc agcctattgc aatcgataca atcgccaata
      601 attccgccgg gatggctgcc ttctcctcgc gcggccccag cgacgacggg cgtatcaagc
      661 cggatatcgt cgcgcctggg actaatatcg tttcgaatgt ctctcacgtg ccgggatcat
      721 ccagtttgtg gggctttttc gagaacaacc cggactatgc ctattctggg gggacatcca
      781 tggcgacgcc gctggcggca ggggcaggcg tgctagtaag acagtggctg gtccagaaag
      841 gtctgtcgaa tcccagcgcg gcagcggtca aagccacgct gctcaatacg accgccgata
      901 ttgctccggg tcaatatggc acagtcgagt tccaggagat accccatagc cgcccaaact
      961 atgtagaggg ctggggacgg gtgaacctgg attttacgat atcagatcca ccgaatcttt
     1021 tctggttgga tgatcgcctg gtgggcttga gcaccggaca agcggtgact tacaacagca
     1081 ccatcactca accactcgag atattgacag acacactgcc ccttagagta atgctcgcct
     1141 ggactgaccc gccggcttcc ctctcggcta cttctcagtt ggtcaatgac ctggacctta
     1201 ccgtgactgg gcctgatggg acagtgtatc acgccaacga caatgctacg ttcgaccgaa
     1261 ccaataatgt cgagggagtg gtgatagata agcctcctct tggagagtat tctattacgg
     1321 tgcatggctt taatgtgcct atttcttccc aaccctatgc tttagcggtt tggggaccgc
     1381 tgcagcctga tatttcgtcc ttttttcaat ccgtgtacct tccggttttg atgaaatgag
     1441 ttcgatttta tataagcaag ctaatcagct tatgagggaa agtcaatccg ccagggtctg
     1501 cacggtgtac agccatgatt atgtgggcga cgcttgtcta tgctaaaaag gtccgcaaga
     1561 gattagaaat aaaggctatc cactcccctg ggcttgctca attctgaata ttcttggttg
     1621 agctcctcga gaatctcctg cttgcggcga gcaacatatt tttccaaaac acgcacttct
     1681 ttaacctttg gaaacaaccg gatacccatt gtggttgagt atcgatctat tgtatttatg
     1741 tagatgatcc tgcccctcaa agctgagaat ttttgtccag gagagagttg gaaatccaat
     1801 cgaatatttg agccggggcg gatattcatc cccttttcga aaagcttata agccaaaagt
     1861 cccaatccat cgaccgagat attctccatg caagcgcgaa gggctttccc tttccaatgc
     1921 aaggtaacat aaatgggaca ttttggctgg accctctcgt gctggcgttt tttccactcc
     1981 atatcggcat aagcgaagtc tgagagcacc aacatacctt tgcgaatatt cagactcttg
     2041 agctgcgcca tcactggttt gggaaataac tggttatgca ggtaaacttc cgtctccgtg
     2101 gcagcgcaca tctcgatgtt ggatgcccga aaggtagcat catctgggct cacctcaagg
     2161 atgctgacat cctgcttaaa gatgatcccc cggaatgatt ttgtcaattc caaggcggcg
     2221 ctttgttgac caagatgcct gataacggat attacatcgt caacgacagg cgagtaattt
     2281 gaggacacgg ataaatcctc ctttggcccc cgtaagccaa tcagttttta gagcgatgga
     2341 tgtaatccac cctcaattga gatggtccat gctcacaaga aagtgtacac aggttccttt
     2401 ataaatacat cataaggcag gattgaggtt ttcttcttaa caaaattgca attatttagt
     2461 catcaatcta agtagattag ctacatagct cgattttatt gaccagcgcc ttcttagagg
     2521 aattgattcc aagcgggatt tacactatac tggatataat ctacagcgtt atggttcatt
     2581 catcccccta gcctgaaagg ggatctggtc aagaaggaaa taatatatga gtacagatga
     2641 cgaacttcgc gaattgttgg atgggactgc agcggaaata tcacgactat ccagtaaccc
     2701 gcgcacctgg t
//
LOCUS       DBNGSX010000611         4014 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_899994, whole genome shotgun sequence.
ACCESSION   DBNGSX010000611 DBNGSX010000000
VERSION     DBNGSX010000611.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_899994"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="AC16CB_17675"
     CDS             <1..241
                     /locus_tag="AC16CB_17675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344356.1"
                     /translation="VFYGMDNNVDRRQGISRFSQQIIDQSQDANSLHDLLKAHNIGYV
                     YIGARGGVLSPRRLQNNPLYQTLYHQDGTWVFKVK"
     gene            274..1248
                     /locus_tag="AC16CB_17680"
     CDS             274..1248
                     /locus_tag="AC16CB_17680"
                     /inference="COORDINATES: protein motif:HMM:NF014476.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HIR3344357.1"
                     /translation="MDAPQLLYLSRKDVERVALDMATIIQLLESAFREKGEGRVEMPP
                     KPGIHTQPDAFIHAMPAYIPGLRSAGIKWVSGYPASQALGLPYISGLLILNDDETGLP
                     YAVMDCTWITAYRTAAATALSARFLARPESSVAGILACGVQGRTNLEALKVIFPIQRA
                     YAYDIVPEVQKRYVEEMSKKLDLEIIGVDDPHKAVAESDLVVSSGPILKHPQPTIQKD
                     WLKPGSFGSAVDFDSYWHPSAMAQMDKIATDDHSQFQYYRSVGYFGQTPDPYADLGEI
                     VAGFKPGRQSDDERTLAINLGLALDDMAVAPEIFRRAKELGLGVWLPL"
     gene            1261..1941
                     /locus_tag="AC16CB_17685"
     CDS             1261..1941
                     /locus_tag="AC16CB_17685"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3344358.1"
                     /translation="MAIDKYPRPSLTVDIVIFTLRENRLQVLLIRRANQPFAGYWALP
                     GGFVQVDESLEDAAARELSEETGIEQAYLEQLYTYGDPERDPRGRVVSVAYFALIPAD
                     ASVRSEGGSDASQASWFPLDELPDLAFDHAEIISYAIRRVRYKLEYTAVGFELLPESF
                     TLSEIQKTYEIILGEPLDKRNFRRRMLESGIIEPTQFKRGKGGRPARLYRYREDAVAE
                     IKARRLFP"
     gene            1956..3170
                     /locus_tag="AC16CB_17690"
     CDS             1956..3170
                     /locus_tag="AC16CB_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3344359.1"
                     /translation="MPAPRLHRLPTNVWVLSAASFLTDVSSEMLNNLLPLFLYNVLGV
                     GTAIIGLIDGIAETTASLVKIYSGVFSDRIGKRKPLTISGYLLSTLAKPFLYLATSWG
                     WVLGVRFVDRIGKGIRTAPRDALLAGSVSTEQRGFAFGLHRASDTAGAFTGLGIAACL
                     IWINQAGKTQLTQATFQWVVLASILPAALAVLILVIGARDVEATKTANSIVPTFRFTW
                     NNLDRRIKAFILVIALFTLGNSSDAFIILRSQERGLSVLQILGMLLTFNAIYTLLSSP
                     AGALSDRIGRKGLILAGWTIYGLVYLCLAFAKTGFQVWLFFSLYGIYYALTEGTARAY
                     IADLVSQDQRGGAYGLFHAAIGLASLPASLIAGILWQGIGPWAGFGPSAPFVFGAGMA
                     ILSVGLLVSWVK"
     gene            complement(3264..3439)
                     /gene="ssrS"
                     /locus_tag="AC16CB_17695"
     ncRNA           complement(3264..3439)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="AC16CB_17695"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3493..>4014)
                     /locus_tag="AC16CB_17700"
     CDS             complement(3493..>4014)
                     /locus_tag="AC16CB_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HIR3344360.1"
                     /translation="PDPDVLAFCWVIDFPFVNWNEDEQRWDPSHHLFTSPMLEDIPFL
                     DNEPGKARGQQYDLVLNNYEVGGGSIRIHDRGLQERVFQLIGLDAEVAQQRFGHMLEA
                     FEFGTPPHGGIAPGIDRICMILADERNIREVIAFPKNQAARDVMGNAPSPVESEQLKE
                     LHIKLDLKPERPN"
BASE COUNT          938 a         1054 c         1015 g         1007 t
ORIGIN      
        1 agttttttac ggtatggaca acaatgtcga tcgaagacag ggaatctctc gcttcagcca
       61 gcagatcatc gatcaaagcc aagacgccaa ttccctgcat gatttgctaa aagcacacaa
      121 tattggctat gtctacatag gcgctcgcgg aggtgtgtta tcaccgcgca gattgcaaaa
      181 caatcctcta tatcagaccc tctatcatca ggatggtacc tgggtattta aagtaaaata
      241 gggtagggac aaattcgcta ccaggaggct accatggatg cacctcaatt gctctatctc
      301 tcccgcaaag atgtcgagcg ggttgctctt gacatggcaa ccatcatcca gctactggaa
      361 agtgcttttc gcgaaaaagg cgaggggcgc gtggagatgc cccccaaacc gggtatccac
      421 acccagccgg atgccttcat ccacgcaatg ccagcctata ttcctgggct gcgatccgca
      481 ggaattaaat gggtgagcgg ttatcctgcg agccaggcgc tcggcctgcc ctatatcagt
      541 gggttgctca tcttgaatga tgacgagact ggcctgccgt acgcagtcat ggactgcacc
      601 tggatcaccg cctaccgcac ggcagccgct actgctctgt ctgcccgctt tcttgctcgc
      661 cctgaaagca gtgtagcagg tatcttagcc tgcggcgtgc aaggtcgcac caatcttgaa
      721 gcgttaaaag tcattttccc catccagaga gcctatgcat atgacattgt gccagaggtt
      781 cagaaacgtt acgttgaaga gatgtcgaaa aagctggacc ttgagattat cggtgtggac
      841 gacccgcaca aggctgtcgc agaaagcgat ttggttgtat cctccggccc gattctcaag
      901 cacccacaac caaccattca aaaagactgg ttaaaaccag gctcatttgg cagtgccgta
      961 gattttgaca gttactggca tccatccgcc atggcgcaga tggataaaat tgccacagat
     1021 gatcattccc aatttcaata ttatcgctcg gttggctact ttgggcaaac accagatcca
     1081 tacgctgacc tgggtgagat cgtggctggg ttcaaacctg gtcgccaaag cgacgatgag
     1141 cgcaccctgg ctatcaacct cggccttgcg ctggatgata tggctgtagc ccctgagata
     1201 tttcggcgtg ccaaagaact tggattggga gtctggctgc cattatagag agacgattgc
     1261 atggccatag ataaatatcc tcgcccttcg ctcaccgtcg acattgtgat cttcacactg
     1321 cgtgaaaatc gccttcaagt attgctcatc cgacgtgcaa accagccatt cgctggctac
     1381 tgggcgttac ctggtggatt tgtacaggtt gacgaatcct tggaagatgc tgcggcgcga
     1441 gaattaagcg aggagactgg tattgagcaa gcctacctgg aacagcttta cacctatggt
     1501 gatccagaac gcgatccccg tggacgggtg gtcagtgtag catacttcgc tctgattcca
     1561 gcagatgcat ctgttcgcag cgaaggtggt agcgatgcca gccaagctag ttggttccca
     1621 ttggatgagt tgcctgatct agccttcgat cacgccgaga tcatctctta tgctattcga
     1681 cgcgtgcgtt ataaacttga atataccgcc gttgggtttg agctcttgcc agaaagtttc
     1741 acgttgtctg aaatccagaa aacgtacgag atcattctcg gcgagccatt ggataagcgc
     1801 aacttccgca ggcgtatgct tgaatctggc atcatcgaac caacccagtt caagcgaggc
     1861 aaaggaggtc gtcccgcgcg cctctaccgc taccgggaag atgcggttgc cgagattaaa
     1921 gccaggcgtt tattcccgta aagatctaat cccatatgcc cgcaccacgc ctccatcgct
     1981 tgcctaccaa tgtctgggtg ctatcagcag cctcattcct gaccgatgtt tcttcagaaa
     2041 tgctcaacaa cctgttaccg ttgtttctat ataacgtatt gggtgtgggc acagccatta
     2101 taggcctcat tgacggtatc gcagagacca ccgccagtct agtcaaaatt tactcagggg
     2161 tattctctga tcgcattggg aaacgaaaac cgctgacgat atcaggttac ttgctttcca
     2221 ccctggcgaa accatttcta tatctcgcca ccagttgggg atgggttctg ggtgtccgct
     2281 ttgtcgatcg gattggtaaa ggcatacgta cagcccctag agatgcttta cttgccggaa
     2341 gtgtctccac agaacagcgc ggctttgcct tcggtctgca ccgagccagc gatacagcag
     2401 gggcattcac ggggctgggc atagctgcct gtttgatctg gatcaaccag gcaggcaaaa
     2461 ctcagctcac acaagctaca ttccagtggg tcgtgctggc gagcatattg cctgcagcac
     2521 ttgccgtctt gatccttgtc attggagctc gcgatgtcga agctacaaaa acagcgaatt
     2581 ccatcgtccc aacatttcgc tttacgtgga acaacttgga tcggcgcatc aaagccttta
     2641 tcctggtgat tgccctgttc acgttgggta actcttccga cgcctttatt attttacgca
     2701 gtcaggaaag aggtcttagc gtgctgcaaa tcttggggat gctgttgacc tttaacgcca
     2761 tctacacact gctatccagc ccagcgggtg ccttgtctga tcgtattggt cggaaagggt
     2821 tgattctggc tggatggacc atctatggtt tggtatatct ttgcctggca tttgccaaga
     2881 ctggcttcca ggtgtggctg tttttcagct tgtacggcat ctattatgca ctgaccgaag
     2941 gcaccgccag agcttacatc gccgaccttg tctcacaaga ccagcgcggg ggagcatacg
     3001 ggcttttcca cgccgccatt ggactggcat cactgccagc atctcttatc gctgggatcc
     3061 tatggcaagg cataggaccc tgggcagggt ttggtccaag tgcgccattt gtattcggcg
     3121 caggcatggc catcttgtca gttgggcttt tagtgagctg ggttaagtaa atttgctctt
     3181 gcttcgaaaa aaaacacctc tcgaggtcaa ttggcctcga gaggtgtcgt ctttctggat
     3241 atcacctagc ggcgattcag aaatgacacc ccactgtgcc gtgtgcagct aagttttgaa
     3301 cctgccttcg caggttggga cccgccccga gtgctccgcc ttggctcagg cctatcgcgt
     3361 cacactcgca agattgggac ccgatttgtg ggtgttggct caaaaccggg ttgcagcaac
     3421 acgcacacag cagggttatt tatcttatac cataaaacca tttgcgtgaa taggtaaaac
     3481 gagattaata atttagttag gtcgctcggg cttcaagtcc agcttgatat gaagctcctt
     3541 aagctgttcc gattctacgg gggagggagc attacccata acatcacgtg ctgcctgatt
     3601 tttagggaat gcaatcactt cgcgtatatt gcgctcgtca gccaggatca tacagatgcg
     3661 gtcaatacct ggagcgatcc caccatgagg cggcgtgcca aactcaaatg cctcaagcat
     3721 gtgcccaaaa cgctgctggg ctacctctgc atctagtccg attagctgga aaactctttc
     3781 ctgtaatccg cggtcatgga tgcggatcga tccaccgccc acttcatagt tattcaacac
     3841 caggtcatat tgctggccgc gcgctttgcc gggctcgtta tccaagaagg ggatatcttc
     3901 tagcattggt gatgtgaaga ggtgatggct gggatcccaa cgctgctcat cttcgttcca
     3961 gttgacaaac ggaaaatcta tgacccaaca aaatgccaac acatctggat ctgg
//
LOCUS       DBNGSX010000612         2551 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1868505, whole genome shotgun sequence.
ACCESSION   DBNGSX010000612 DBNGSX010000000
VERSION     DBNGSX010000612.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1868505"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            319..972
                     /locus_tag="AC16CB_17705"
     CDS             319..972
                     /locus_tag="AC16CB_17705"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR3344361.1"
                     /translation="MFNALDLAPSILISHLVVLIVAFTIHEFAHAWTANYFGDTTPEA
                     NGRLTLNPLAHLDPIGSLLLLVAGFGWAKPVPVNPYQLQRRSPSAMMWVSLAGPLSNL
                     LLAIVAAIPFRLGVFSVLDAQMALFTARDQFLPTLPQLLYIFIQINLVLLLFNLIPLA
                     PLDGEKIAEYFLPASWLRTLDRIRPYGPVILMVIVFFGVLTYIITPPLRLLMNLLVG"
     gene            969..1511
                     /locus_tag="AC16CB_17710"
     CDS             969..1511
                     /locus_tag="AC16CB_17710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344362.1"
                     /translation="MIAERILYRARQFWNALFSAPSLEDLETISPLLNPAQMALFSQM
                     HRSEQVHSIKVMRTVMVASDGVSAELRKNLLQAALLHDVGKNHYPLRLWERVLIVLAK
                     TLTPNRVKDWGEGEARGWRRAFVIASQHPAWGAQMASAAGASPLTVSLIQRHQDLLSE
                     AGEALEDQLLRLLQSADHEE"
     gene            1524..2519
                     /locus_tag="AC16CB_17715"
     CDS             1524..2519
                     /locus_tag="AC16CB_17715"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HIR3344363.1"
                     /translation="MTTQITIVGLGQIGASIGLALAEQEQLFQRIGHDRDLETARRAQ
                     KMGAIDKVAINLPSAVREADLVILSVPADQLRETLEIIAQDLKENAVVMDTGPIKVSM
                     SEWIKELLPDNRFYVGLVPSINPAYLYQEEIGIDAAHADLFQRGIMAIITPPNTSSDA
                     LQLASKLASLLGSDPLFVDPLEIDGLLAATHALPQLLAVALLNATVSQPGWREARKIA
                     GRAYADVSAASLHAGMADGLGGMTLHNRDNVVRVLDRAIASILSLREAIEANDGEFLV
                     RQLESAQQGYAEWLKERLAANWAFKGMPESVSKSSGRVDFLSRLLGSGVKRKDRK"
BASE COUNT          581 a          631 c          666 g          673 t
ORIGIN      
        1 agcacttctt cggtatcttg ttggtaccat gttcccataa tgcctcacaa aggacaaaat
       61 cagcttgatt gtaccttatt tatatctgca ttttgcactg ttactatgcc cttgcgtgca
      121 tgtgtaatcc aacaaaactt ggatttaggg tcttcttgtg ggggcatact ctcaccagga
      181 taccccaatc tctccctcca gtggcaggaa atcgctctaa tgcgattgct ctagattgcc
      241 ctgtgccctg gcatgctcca ggaggttaga agaaaggctg tggtcgaaaa tggtataatc
      301 ctctcattct ggaggaatat gtttaatgct ttagatttag ctccatcaat attgatatct
      361 catttagtag tattgattgt ggcttttacc attcacgaat ttgctcacgc atggacagcc
      421 aattattttg gcgataccac acccgaagcc aatggtcgcc tgacgttgaa ccctctggcg
      481 cacctggatc ctatcggatc gctgctgttg ttggtggctg gatttggttg ggcgaaacct
      541 gtgccggtca atccatatca attgcaaaga cgctccccgt ctgccatgat gtgggtctcg
      601 ttagctggcc cgctctcgaa tttgctcctg gcgattgtgg cagccatccc gtttcgttta
      661 ggtgtttttt cagtgctgga tgcacagatg gcgttgttta ctgccaggga ccaattttta
      721 cccacgctac cacaacttct atatatattt atccaaatta atttggtatt gctcctgttc
      781 aacttaatcc cactggcgcc tttggatggg gagaaaattg ccgagtactt cctcccagct
      841 tcttggttgc gcaccctcga ccgcatccgt ccatacgggc cggtcatcct gatggtcatt
      901 gtgttcttcg gtgtgctgac ttacattatc acacccccac tcaggctgtt gatgaaccta
      961 ctggtgggat gattgccgaa cgtatcctat accgcgcccg acagttctgg aatgccctgt
     1021 tttcggcgcc ctcgcttgag gatcttgaga cgataagccc gctactcaat cccgcgcaaa
     1081 tggcgctctt tagccagatg caccgcagcg agcaagtgca cagcatcaag gtgatgcgga
     1141 ctgtgatggt tgcaagtgat ggggtctcag ccgagctgcg aaaaaatttg cttcaggcgg
     1201 ccttgcttca cgacgtaggt aaaaatcatt atccactcag gctgtgggag cgggttttga
     1261 tcgtcttagc gaagacgctc acacccaaca gggttaaaga ctggggagaa ggagaagcgc
     1321 gtggttggcg gcgtgcattt gtgatcgcca gccagcatcc ggcatgggga gctcaaatgg
     1381 catctgcggc tggagcttcg ccactgacgg tttctctgat acaaagacat caagatcttc
     1441 tttccgaagc cggggaggcg ttagaagatc aattactccg attattacaa tccgcagatc
     1501 atgaggaata ggatgaatta tctatgacga cccaaataac cattgtaggt ttaggacaga
     1561 tcggagcatc aattggattg gccttggccg agcaggagca gttgttccag cgaattggcc
     1621 acgatcgaga cctggagact gcccgccgcg cccagaaaat gggtgccatt gataaagtcg
     1681 ccatcaattt gccgtccgcg gtgcgcgagg cggacctggt tatattgtct gtacctgcag
     1741 accaattgcg cgagacctta gaaatcattg cccaggatct aaaggaaaac gcggtggtga
     1801 tggataccgg tccgattaag gtttcgatgt ctgagtggat aaaagagttg ctaccagaca
     1861 atcgcttcta tgtggggctg gtaccatcta taaatccggc ttatttatat caagaagaga
     1921 tcgggatcga tgccgcccat gctgacttat tccagcgtgg tatcatggcg attatcaccc
     1981 cacccaatac atccagcgat gccctccagt tagccagcaa actggcgagc ctgctgggtt
     2041 cagatcccct gtttgtcgac ccattggaaa ttgacggctt actggctgcc acgcacgctt
     2101 taccccaact gttggctgtt gcgttgctca atgccacagt tagtcagccg ggttggcgtg
     2161 aggcgcgcaa gatcgccggg cgggcttatg ccgatgtatc tgctgccagc ctgcatgccg
     2221 gtatggcaga tggcttgggt ggcatgacat tgcacaaccg tgataatgtg gttcgtgtcc
     2281 ttgacagggc tatcgcctca atactatcgc tgcgagaagc catcgaagcg aatgatggcg
     2341 agtttctagt gaggcagtta gaatctgccc agcaaggtta tgctgagtgg ttgaaagagc
     2401 gactggccgc caactgggct tttaaaggca tgcccgaatc tgtatcgaaa tcgtcaggga
     2461 gagttgactt cctatcccgc ttgcttggtt cgggggtcaa gcggaaggat cgtaaataaa
     2521 ttgcgaggga aaaccatgac taccaggatt c
//
LOCUS       DBNGSX010000613         2884 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1730403, whole genome shotgun sequence.
ACCESSION   DBNGSX010000613 DBNGSX010000000
VERSION     DBNGSX010000613.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1730403"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="AC16CB_17720"
     CDS             complement(<1..512)
                     /locus_tag="AC16CB_17720"
                     /inference="COORDINATES: protein motif:HMM:NF012267.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR3344364.1"
                     /translation="MSEENCTGCGTCVEKCPWKKIPSEFDLGLGTRPAIYFPFAQAVP
                     RVPVIDTQNCAYFLRGKCRACEKFCQREAIDFEQQDQLIDVQVGTIILATGFKDFDPR
                     QSPKYEFSNLDNVVTGMELERLINTGGPTTGNVLLKDGRKPKSIGLLHCVGSRDPEHH
                     EYCSRVCCMYS"
     gene            complement(642..1280)
                     /locus_tag="AC16CB_17725"
     CDS             complement(642..1280)
                     /locus_tag="AC16CB_17725"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3344365.1"
                     /translation="MSSTDNMIFESSLSQNGRVKPRIGVYICHCGTNIAGMIDVSSVA
                     DYAATLNNVIVSRHYPYMCSEPGQDLIKQDILHEGLNRVVVASCSPLMHETTFRRACE
                     EAGLNPYLFQMTNIREHCSWVSDDPEKATQKAKALVSAATKRVALNTELTTRRVDVQQ
                     AALVVGGGIAGIEAALRLADAGKQVYLVERNPSIGGHMAQLYKTFPTLDCAA"
     gene            1486..2877
                     /gene="cysS"
                     /locus_tag="AC16CB_17730"
     CDS             1486..2877
                     /gene="cysS"
                     /locus_tag="AC16CB_17730"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR3344366.1"
                     /translation="MNLRLYDTYTRSLREFEPIHPEEVGLYTCGPTVYDYAHIGNFRT
                     YIFTDILRRVLAINGYSVKHVMNITDVGHLTSDADEGEDRMEKGSRRTGMTAWEIAEL
                     YTQEFKLDLERLNILEPTIWCRATDHISDQIEVIQCIEKNGYTYRTSDGIYFDTSKMR
                     DYGLLARLDIEGLQAGARIDIGEKRHLTDFALWKFSTSDQKRQMEWDSPWGVGFPGWH
                     IECSAMSAKYLGPLFDIHCGGEDHISVHHTNEIAQTQACYGTRLANFWMHGYFLQIDE
                     ARMGKSAGNFLRLQTLIDMGYDPLAWRMFCLNAHYRAKLNYTVDGLDGAMISLDRLRK
                     AAYDWGEPSMVDEEYVAKFLETVNDDLNMPRAMALTWDLVKSDLPNGTKKATILYFDQ
                     ILGLRLDEWEPIEEIIPDEIMELVQKRQEARQDKRWKDADALRDEVSVAGFEIEDTPQ
                     GPRVKRRKIRIEN"
BASE COUNT          744 a          645 c          727 g          768 t
ORIGIN      
        1 gagtacatac aacataccct tgaacaatat tcatggtgct cagggtcgcg gctgcccacg
       61 caatgtaata atccaatgct ttttggcttg cgtccgtcct ttagcagcac atttccagta
      121 gttgggcctc cggtattgat caggcgctca agttccatac cggtcactac attgtctagg
      181 ttgctaaact catacttcgg tgactggcgt ggatcgaagt ctttaaaacc ggtcgcaagg
      241 ataattgtcc ctacctgaac atcaatcaat tgatcttgtt gctcaaaatc gatggcttct
      301 ctttggcaaa acttttcgca ggcgcggcat tttccccgca gaaaatatgc gcagttctgc
      361 gtgtcgatca caggcacgcg cggtactgct tgggcaaaag gaaaatatat cgctgggcga
      421 gtgcctaatc ctagatcgaa ttctgagggg atttttttcc agggacactt ctctacgcac
      481 gtaccgcatc cggtacaatt ttcctcactc acgtaacggg ctttctttct cacgcttact
      541 gtgaagttgc ccacatagcc cgaaactgat tcgacctcgc tgttgcttag tagtctgatg
      601 ttttcgtggc gtccaacgga caccattttg ggggtgagaa ttcaagccgc acagtccaat
      661 gtcgggaacg ttttgtagag ttgagccata tgtccaccga tgctggggtt gcgctctacc
      721 agatagactt gctttcctgc atcagctaag cgcaatgcgg cctcgatccc agctattcca
      781 ccacccacga ccaatgcagc ctgttgcacg tcaacccgtc gtgtggtgag ctccgtattc
      841 agtgcgacac gcttggtagc agctgatacc aacgccttgg ctttttgggt tgctttttct
      901 ggatcatcag agacccaaga acaatgctcg cgaatgttgg tcatttgaaa cagatatgga
      961 tttaaccctg cttcctcgca agcacgccga aatgttgtct cgtgcatcaa tggagagcat
     1021 gaggcgacca ctactcgatt cagtccttcg tgcaggatat cttgcttaat gaggtcttgg
     1081 cctggttcag aacacatata ggggtagtgt ctggaaacga tcacattatt taaagttgcg
     1141 gcatagtccg caacactgct gacatcaatc attccagcga tgtttgttcc acaatggcag
     1201 atgtatactc cgatacgggg tttaacgcgt ccattttgac tgagtgacga ttcaaatatc
     1261 atgttatccg ttgaactcat gcggaagtct ccttatttgt ccccacccga tccaatatca
     1321 gttgatgatg catctcgttc atcagtgtaa ttagaagaac aaccagatat attataacac
     1381 agattgtgca aaaattcaca attgaaaaat attctttttc ctctcaatat ttgttttgcg
     1441 taattgcggt aaaatagcga agatcaattc agcaaagggt aaagaatgaa tctcagattg
     1501 tatgacactt acacgcgcag tttgcgggag tttgagccga tccacccgga agaggtgggt
     1561 ctgtatactt gcggcccgac ggtatatgac tatgcccata ttggcaattt ccgcacctat
     1621 atctttacag atatcttaag acgggtgtta gctatcaatg gatattcggt caaacatgtg
     1681 atgaatatta ctgacgtggg ccacctgaca tcagatgctg atgaggggga agatcggatg
     1741 gaaaaaggct ctcggcgcac gggaatgacg gcctgggaga tagctgagct gtatactcaa
     1801 gaattcaagt tggacttaga acgccttaat atccttgaac caacgatctg gtgccgcgcg
     1861 accgatcata tatccgatca aatcgaggtc atccagtgca ttgaaaagaa tggttataca
     1921 taccgcacat ctgatgggat atattttgat acctcgaaga tgcgtgatta tgggctcctg
     1981 gcccgcttag acattgaagg cttgcaagca ggggcgcgta tcgacattgg agaaaagcgt
     2041 cacctaacgg atttcgcgct gtggaaattt agcacatcag atcaaaagcg acagatggag
     2101 tgggacagtc cttggggagt aggtttcccg ggatggcata tcgaatgctc ggccatgtct
     2161 gctaagtacc tgggaccact ttttgatatt cattgtggcg gcgaggatca tatcagtgtg
     2221 caccatacca atgagatcgc ccaaacgcag gcttgttatg ggacgcgcct ggcgaatttt
     2281 tggatgcatg gatatttcct tcagattgat gaagctcgga tgggtaaatc tgctggcaat
     2341 ttcctgcgtc tgcagacgct gattgatatg ggatacgatc cgctggcgtg gcgcatgttt
     2401 tgcttgaatg ctcactaccg tgcaaaattg aactatacag tggatggctt ggatggagcg
     2461 atgatttcgc tggaccgcct tcggaaagca gcttatgatt ggggtgagcc aagtatggtg
     2521 gatgaagaat atgtggctaa atttttggag actgttaatg atgacctgaa tatgccgcgt
     2581 gccatggcat taacatggga tttggtaaag tccgacttgc ctaacggaac caagaaagct
     2641 acgatccttt attttgatca gattttaggt ctccggctcg acgaatggga accaattgag
     2701 gagatcatcc cggatgagat catggaattg gtacaaaaac ggcaggaagc ccgccaagat
     2761 aaacgctgga aggatgccga tgcgctgcgc gacgaggtga gcgtagctgg atttgagatt
     2821 gaagatacac cacagggacc ccgggtaaag cgcaggaaaa taaggattga aaattagaga
     2881 cggg
//
LOCUS       DBNGSX010000614         2853 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_900174, whole genome shotgun sequence.
ACCESSION   DBNGSX010000614 DBNGSX010000000
VERSION     DBNGSX010000614.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_900174"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="AC16CB_17735"
     CDS             <1..555
                     /locus_tag="AC16CB_17735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344367.1"
                     /translation="RVIGLFVIACLIFSLGSSPVSAASDLQIDIDMDYTVVKTGDKVE
                     FNTLVTNNDSEVSPPLIVAMNVINLDAKGDVVDPEDWSPQRTQYIDSLGSGESTNLGW
                     IINTILDGDYMVYMVLIPAPSSTESTSQPIASSGIHLTVTPFTRLNPGGVLPYVIGAP
                     LLLMIVIILVYRRRRQAIDMGDAS"
     gene            596..1381
                     /locus_tag="AC16CB_17740"
     CDS             596..1381
                     /locus_tag="AC16CB_17740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344368.1"
                     /translation="MNATQRWIFVILIMAGLQLSACKPASKMVEKIQPAQLEPIEGSD
                     FKRVILTEKAAERLDIQTVTVREEQIERKLTVGGQIMTVSEDSALSDNVTAVAGGIKE
                     ALLVRIPLSQNELKIVDRSQPVYIQSLDDEEDFDDEIDDLYAEWDEGLQDDDDEDDAL
                     YYMVNGAKNGLLPGHGVLVELSLVGGATTQMVVPYAAVLYGVHGETWVYTNPEPLVFV
                     RQPIVIDFIDGDMAVLSEGPEAGTEIVTIGAAELFGAETGVSK"
     gene            1401..>2853
                     /locus_tag="AC16CB_17745"
     CDS             1401..>2853
                     /locus_tag="AC16CB_17745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3344369.1"
                     /translation="MRWIVQTSLRYRYLVVFISIALMIFGFARLSGSSVDVFPEFAPP
                     KVEIQTISLGLSAADVEELVTVPLEQALNGVPGLDVMRSRSVPDLSDIVLIFKPGTDL
                     IFARQLVAERIAAIYPTLPTWAAPPVMIQPLSSTSRAMKIGISSDQYDLMDLSMIAYW
                     KIRARLLDVPGVANAPIWGERIKMPQVQVDLERMRAYNVSLNEVMDVTADALDVGLLT
                     YSEGSVIGTGGFIDTPDQRYGIRSVLPVITPEKLGEVPINDRIKPDGTPLLIKDIGDV
                     VWDTWPLFGDAVINDGYGLMMIVEKLPWANTLDVTRGVEEAIEKMKPGLKGIDIDTTI
                     FRPATFIEQSIDNLTESLLIGALLVVVVLLFFLYEWRVALISATIIPVSLMASLLVLN
                     LRGVTINVMTLAGLAIAIGAVVDDAIVDVENIVRRLRRLRKEGSTQSTASIVLEASLE
                     VRGAIVYASLIEISALLPVFFLEGLSGAFFQPLA"
BASE COUNT          648 a          688 c          788 g          729 t
ORIGIN      
        1 cgtgtaatag gtctttttgt gatcgcctgc ctcatcttct ccctggggtc atcgccggtt
       61 tcagcggcct cagatctgca gatagatatc gacatggatt acacggtagt gaagacggga
      121 gacaaagttg aattcaatac gctggtaacc aacaatgatt cggaggtttc tcctcctctc
      181 attgttgcga tgaatgtcat caatctagat gccaaaggtg atgtggttga tccagaagat
      241 tggtcgccgc aacgcaccca atatatcgat tccctgggat cgggcgagtc cacaaatctg
      301 ggatggatta tcaataccat tctggatgga gactatatgg tgtatatggt tctcatccct
      361 gcaccaagta gcacggagtc caccagccag cctattgcca gttcaggtat ccacctgaca
      421 gtgacgccat ttaccaggct aaacccaggc ggtgtacttc cctatgtcat cggagctccg
      481 ttgctgctga tgattgttat tattcttgta taccggcggc ggagacaggc aattgacatg
      541 ggtgatgcct cataagaatg ttcgaggctg tgctgtaaat taggaggaaa taaaaatgaa
      601 cgccactcag cgatggatct ttgtgatttt gatcatggcg ggattacaac tttcggcctg
      661 caaaccggca tcgaagatgg tagagaaaat tcagcctgct cagcttgagc caatcgaagg
      721 ttcagatttt aagcgcgtga tcttgactga gaaggccgcc gagcggctcg acatccagac
      781 agtcacagtt cgtgaagagc agatcgagcg gaagctaacc gttggaggtc agatcatgac
      841 ggtgagcgag gattctgctc tatccgacaa tgtaactgca gtcgctggtg gcataaaaga
      901 agccttactg gttcgtattc cccttagtca gaatgaacta aaaattgtag accgaagcca
      961 acccgtgtat atccaatccc tagacgatga ggaagatttc gacgacgaaa tagatgactt
     1021 gtatgccgaa tgggatgaag gtctacagga tgatgacgat gaggatgatg cgctgtatta
     1081 catggtaaat ggtgcaaaaa acggcctgct tccggggcac ggtgtcttgg tcgagctttc
     1141 cctagtgggt ggcgcaacga cacagatggt cgtcccttac gctgcagtgc tgtatggagt
     1201 acatggcgag acctgggtat acactaaccc cgaacccctg gtatttgtcc ggcagcctat
     1261 tgttatcgac ttcatagatg gggacatggc agtcttgtcg gaaggaccag aggctggtac
     1321 tgagattgta acaattggag cagctgaatt gtttggtgcc gagaccgggg tttcaaagta
     1381 acagagcagg gaggtatatc atgcgatgga ttgtacaaac aagcttgcgc tatcgctatc
     1441 tggtggtgtt tatttccata gcgctgatga tcttcggctt tgctcgccta agtggctcaa
     1501 gtgtagatgt ctttcccgag ttcgcgccgc ctaaggtgga gattcaaacc atctccctcg
     1561 ggctttccgc ggccgatgtc gaggaactgg ttactgttcc gttggagcaa gcgctcaatg
     1621 gagttccggg tttggacgta atgcgctcca ggtcagttcc tgacctgtcg gatatcgtgc
     1681 tcatctttaa gcccgggact gacctaattt ttgcacggca gctggtggcg gagcgcatag
     1741 cggcgattta ccccacactc cctacctggg ctgccccacc ggtcatgatc cagcccctct
     1801 cgtcaaccag ccgggcgatg aagattggaa tatcgtcaga tcaatatgat ctgatggatc
     1861 tttcgatgat cgcttactgg aagatcagag cccgcttgct ggatgttcct ggagtggcta
     1921 atgcgcctat ctggggcgag cgtatcaaga tgcctcaggt gcaagtcgac ctggaacgca
     1981 tgcgggctta taacgtttcc ctcaacgagg ttatggacgt taccgccgac gcgctggatg
     2041 tgggattatt aacctattcg gaaggatcgg tcattggcac tggaggcttt attgatactc
     2101 ccgatcagcg ctacggcatt cgttccgtat tgccggtcat aacacccgaa aagctgggcg
     2161 aggtgcctat caatgatcgc attaagcccg acggcacgcc gttactcatc aaggacattg
     2221 gcgatgtggt ttgggatacc tggccactct ttggagacgc ggtcattaac gacggctatg
     2281 gtctcatgat gatcgtcgag aagcttccct gggctaatac cctggacgtg acgcggggcg
     2341 tggaagaggc gatcgaaaag atgaagcccg gccttaaagg tatcgatatt gacaccacca
     2401 ttttccgacc ggcaaccttc atagagcaat cgatcgataa tcttaccgag tctttgctca
     2461 tcggcgcgct tcttgtggtg gtcgtgctgc ttttcttcct gtatgagtgg cgagtggctt
     2521 tgatcagtgc cacgattatc cccgtatctt taatggcctc cttgcttgtg ctcaacctac
     2581 gcggcgttac aatcaatgta atgactttgg ccgggttggc gatcgccatt ggggcagtcg
     2641 tcgacgatgc catcgttgat gtagaaaata tcgtgcgacg cctacgccgg ctgcgcaagg
     2701 agggcagtac ccagtcgacg gcaagtattg tcctggaggc ctcgctcgaa gtgcgcggtg
     2761 ctatcgttta tgcctcgctg atcgaaatct cagctttgct gccggtcttc ttcctggagg
     2821 gcttgtcggg agcgttcttc cagccattgg cca
//
LOCUS       DBNGSX010000615         1973 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1245689, whole genome shotgun sequence.
ACCESSION   DBNGSX010000615 DBNGSX010000000
VERSION     DBNGSX010000615.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1245689"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="AC16CB_17750"
     CDS             <1..335
                     /locus_tag="AC16CB_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR3344370.1"
                     /translation="SSADFLHGPLAVIEHGFPAIIIAPSGAMLPELQEFMRTIQQRQA
                     EILAISDDDEILGLSDIPLRLPIAVPEWLSPITAIIPGQLLAMHLASIRNYDPDHPRG
                     LRKVTETV"
     gene            455..1765
                     /gene="melA"
                     /locus_tag="AC16CB_17755"
     CDS             455..1765
                     /gene="melA"
                     /locus_tag="AC16CB_17755"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010496524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HIR3344371.1"
                     /translation="MIKVSIIGAGSNEFSAELTRDILATPALSQGTFALIDIDAERLE
                     LAHRMVERLIEQTGRNWTVEASIERKDIMAGSDYIINAIEVAGLANVRHDYDIPMKYG
                     VDQCIGDTSGPGGLFKALRTLPAWIEILKDAERLAPNCLVMNYTNPMSLTVLAGLRAT
                     RLPIVGLCHSVQHTSMQLSSYLDIPYPQIRWQVAGINHLAWFVELSRDGEDLYPRLRQ
                     RARLPEVYEQDPVRLEIMLHLGAFPTESSGHVSEYVGYFRKRPDLLQKYMRNGYRGES
                     GFYANNWPAWRQRGDQRTRAYLRGDGSYPLERGVEYASYIIEAIETNLPTVIYGNVLN
                     SGLISNLPQDGVVEVACLVDKRGVQPTHHGPLPAQLAALNYPHMAFHNLVVTAVLERD
                     REAAIHALMVDPLTSAVCSLEEIRRMFEEMVEAQKAYLPEFLAA"
BASE COUNT          423 a          563 c          551 g          436 t
ORIGIN      
        1 atagcagcgc cgattttcta catggtcctc tggcagtgat agagcatggc tttcccgcca
       61 tcatcatcgc gcctagcggg gcgatgttgc ccgagctgca agagttcatg cgcacgatac
      121 agcagcgtca agccgaaata ctggcgatca gcgatgacga tgagattctg gggctatccg
      181 acatccccct gcgactgcct attgcggtgc cggaatggct ctctccgatc actgctatca
      241 tccccgggca gctcctggct atgcacctgg catccattcg gaactacgat ccagatcatc
      301 cacgtggcct gcgcaaggta acggagacag tgtgacctta gcccccaccc ccgacacctt
      361 ccccatacgg gggaggggag tataagccta atttccatca cattttccca ttcccagctt
      421 tggtggatct tatatgtcca ccacaggaga ttttatgatc aaagtgtcga tcattggcgc
      481 gggcagcaac gagttttccg ccgagctgac ccgcgatatc ctcgctaccc ctgctcttag
      541 ccaggggacg tttgctttaa tcgatattga cgctgaacgc ctggagctgg cccaccgcat
      601 ggtggagcgc ttgatcgagc agaccgggcg caattggacg gtagaagcca gcattgagcg
      661 caaagatatc atggctggca gcgattacat catcaatgct atcgaggtag ccgggctggc
      721 caacgtgcgc catgactacg atatccccat gaaatacggc gtggatcagt gcatcggtga
      781 tacctccggc cccggcgggc tgttcaaggc gttgcgcaca ttgccagcct ggatcgagat
      841 cctcaaagac gccgagcgac tggctcccaa ttgcctggtg atgaactaca ccaatcccat
      901 gtcgctcact gtgctggccg ggctgcgcgc cacgcgcttg cctattgtcg ggttgtgcca
      961 ctccgtgcag cacacatcga tgcagttgag cagctacctg gatatcccct acccgcagat
     1021 acgctggcag gtggcgggca tcaaccacct ggcatggttt gtggagctct ctcgcgatgg
     1081 cgaagacctg tacccccgcc tgcgccagcg cgcccgcctg cccgaggtct atgagcaaga
     1141 tccggtgcgc ctggagatca tgctgcacct gggagccttc cccaccgaat cgagcgggca
     1201 cgtctccgag tatgtcggct acttccgcaa gcgccctgat ttgctgcaaa agtacatgcg
     1261 caacggttac cgcggtgaaa gcggcttcta cgccaataac tggccagcct ggcgccagcg
     1321 gggtgaccag cgcacgcgcg cctatctaag gggcgacggg tcttacccgc tggagcgcgg
     1381 cgtggagtac gcctcttaca tcatcgaggc cattgagacc aacctcccta cggtcattta
     1441 cgggaatgtg ttgaacagcg gcttgatcag caacctgccg caggatggcg tggtggaggt
     1501 ggcttgcctg gtggataagc gcggggtaca gcccactcac catgggccgt tgcctgcaca
     1561 attggcggcg ctcaactacc cccatatggc cttccacaac ctggtggtga ctgccgtgtt
     1621 ggaaagggat cgagaggcgg cgatacacgc tttgatggta gacccgctga cctcggcggt
     1681 gtgctcgctg gaagaaatcc gacgtatgtt tgaggagatg gtggaagctc aaaaagctta
     1741 cctgccggag tttttagccg cttaattgct ggggtaacac agttggaaag aggatattgg
     1801 tcgagtgatg cccgcccaaa atcccctcca ttcttctttg tttacgagaa ccataaattg
     1861 attaataatt gctgacaaat tctgattagt acttgccaaa atcgataaat ttgggtataa
     1921 acgaatgggg taatttatat cgatctaggt gaattttttc gacaagactg ttc
//
LOCUS       DBNGSX010000616         4243 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1660547, whole genome shotgun sequence.
ACCESSION   DBNGSX010000616 DBNGSX010000000
VERSION     DBNGSX010000616.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1660547"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1141)
                     /locus_tag="AC16CB_17760"
     CDS             complement(149..1141)
                     /locus_tag="AC16CB_17760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344372.1"
                     /translation="MLRAELQTTRELAEQLLSLAQRLKDPELLLEAHRVLGPNMLWLG
                     ELVLARKHLEQGVALYDPQKHRSHAFVYGQNPAVACLSLAAWPIWGLGYPDQSLRSIH
                     KALTLAQELAHPFSLAYALTWASTVYQLRGELQAVQERAEALIALSTEQGFPFWFAFG
                     TILRGWAMTAQRAGAEGIAQIRQGLISYRATESELLRPYFLSLLAEAHGKVGQIEEGL
                     ALLNEALDTVKRTGERNWEAELHRRNGELLLMGHGHKMVEAEECFRKALDIARRQQAK
                     SLELRAAMSLSRLWQQQGKQGEAYQLLAGIYGWFTEGFDTRDLKEAKVLLEESA"
     gene            complement(1232..3346)
                     /locus_tag="AC16CB_17765"
     CDS             complement(1232..3346)
                     /locus_tag="AC16CB_17765"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3344373.1"
                     /translation="MSTSATNDSQTTAHAFKARLPGAERRQLTVMFCDLVGSTELSAR
                     LDPEDLREVVRSYQEKAADVIQGFDGHIAQYLGDGLLVYFGYPQAHEDDAHRAVQAGL
                     GIVEAMGKLNTRLESEYGVQLAVRLGIHTGPVVVGEMGGGGRHEQLALGETPNVAARL
                     ERLAEPDTVVISAMTKRLVEGAFALEDLGSNSIKGVPELIAVSRVLNPIVAQTDEEEV
                     GTGVITTLVGRDEEIGLLLRRWEQSKEGFGQVVLINGEPGIGKSALADVLREHVASEG
                     YTRVTYRCSPYHTNSALYPVTTHLQRLWRWERDDSPAKKLAKMEQTLQTTSLPLKEVV
                     PLFAALFSLPLPEERYPPLGLTPQQEKQQTQDTLTAWLLEEAEHQPMLVVYEDLHWAD
                     PSTIESIGLLVEQVPTAPLLAVLIFRPQFAPLWPNRSHMTPITLNRLERPQVEAMITQ
                     LASGKALPAEVVHYIVTRTDGVPLYVEELTKMLLESDLLREEADHFALAGTLSGLAIP
                     ATLQDSLMARLDRLPMVREVAQLGAVLGREFAYEMLKALAAVEESTLQERLGQLVSAE
                     LLYQRGRPPNARYIFKHALIQDAAYASLLKRTRQQYHQKTAQMLEAEFPETVEAEPEL
                     VAHHYTEAGLNDQAVGYWLQAGKRAAQRSANAEAINHLNRGIEVLMKLPDTVERARME
                     LDLHVTLGPALTAVKGFASLDT"
     gene            complement(3419..3550)
                     /locus_tag="AC16CB_17770"
                     /pseudo
     CDS             complement(3419..3550)
                     /locus_tag="AC16CB_17770"
                     /inference="COORDINATES: protein motif:HMM:NF024183.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
     gene            complement(3781..>4243)
                     /locus_tag="AC16CB_17775"
     CDS             complement(3781..>4243)
                     /locus_tag="AC16CB_17775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3344374.1"
                     /translation="NINEDIKNKFISYALGVLQTFVGITAVLGGFGLVSDPSGTKMNI
                     PLALLKNLPFNNYLIPGLILLVFNGVGNVLAGIITFLRTRYTGNLAIFFGVFLILYIA
                     IEVWFIGLHNFSQPLYFILGVVELIFGFKLSKSGKTEHEIWIELAGGKLAT"
BASE COUNT          939 a         1225 c         1095 g          984 t
ORIGIN      
        1 ggataagtga aaataggtag gactatgaag agcctccctt taacttatct catcttgggt
       61 cgttgacctc ctggggtaat tcggccaatg ttcctctctg aacctatgta gctcaattaa
      121 ggttttaaca acgttctgtt ctccgccttc acgcggattc ttccaacaag accttggctt
      181 ctttaaggtc cctggtgtca aatccctcgg tgaaccagcc gtaaatccct gctaatagtt
      241 ggtaggcttc tccctgcttt ccctgctgct gccacagtcg actcagactc atcgcggccc
      301 gcagctccaa cgactttgcc tgctggcgac gggcaatgtc gagagctttc cgaaaacatt
      361 cttcggcttc caccatcttg tgcccgtgcc ccatcagcag aagctctcca ttgcgcctat
      421 gcagctctgc ctcccaattg cgctccccgg tcctttttac cgtatccaat gcttcgttca
      481 gcagggcaag cccttcctct atctgcccga ctttcccatg cgcctcagct agcagagaaa
      541 gaaaataggg tcggagtaat tctgattccg tggcccggta ggagatgaga ccctgacgga
      601 tctgggcaat cccctctgct ccagctcttt gcgcagtcat cgcccagcct cgcaaaatcg
      661 tcccgaaagc aaaccagaat ggaaaaccct gctcggttga tagtgctatt agtgcctcgg
      721 cgcgctcctg aaccgcctgt agttcaccgc gtaactgata gacagtggaa gcccaggtca
      781 gagcataagc caggctgaag gggtgagcaa gctcctgagc taatgtcagc gctttgtgga
      841 tgctccgcaa agactggtcc ggatagccaa gtccccatat gggccacgcc gcaagggata
      901 gacacgctac tgcggggttc tggccataca caaaggcatg ggagcggtgt ttctggggat
      961 catagagcgc cactccctgc tccagatgtt tgcgagccag aaccagttct ccgagccaga
     1021 gcatgtttgg ccccaacacc cggtgggcct ccaggagaag ttcagggtct ttgagccgct
     1081 gggccaggct taagagctgc tccgccagct cacgtgttgt ctgcaactcc gcgcggagca
     1141 tatgaaacct ggtcagacca tgtagcaccg gaaagagttg cggggtttct cccacctgct
     1201 gacagagctc ccgggcccgg gcataagccc gttacgtgtc cagagacgca aagcctttga
     1261 ccgccgttaa tgccggaccc agagtgacat gcaggtccag ctccatacgg gcgcgctcaa
     1321 cggtgtcggg cagcttcata agcacctcga ttcccctgtt gaggtggttg atggcttccg
     1381 cattcgctga gcgctgggcg gcgcgtttgc ctgcctggag ccaatagcca accgcctggt
     1441 cattgagacc tgcctccgta tagtggtggg ccaccagctc aggctcggcc tcgaccgtct
     1501 cgggaaactc cgcttccaac atctgggcag tcttttggtg gtactgctgc cgggtccgtt
     1561 tgagcaacga ggcataggct gcatcctgga taagggcgtg cttgaagatg tatctggcgt
     1621 tcggtggccg gccccgctgg taaagaagct ccgcactcac cagctggccc agtctttcct
     1681 gcagcgttga ctcctccaca gcggccagcg ctttgagcat ttcgtaggcg aactcccggc
     1741 ccagcaccgc accgagctgg gccacctcgc gcaccatggg cagacgatcc agtctggcca
     1801 tcagcgaatc ctgcagcgtg gccgggatcg ccagaccgga caaggtgccg gccagggcga
     1861 agtgatcggc ctcctcccgc aaaagatccg attccagaag catcttggtc aactcttcca
     1921 catacagcgg cacgccatcg gttctggtga caatgtaatg gaccacttcg gccggaagcg
     1981 ccttgccgct tgccagctgc gttatcatgg cctccacctg gggacgttca agacggttga
     2041 gcgtaatcgg agtcatatgg gagcggttag gccacagggg ggcaaactgc gggcggaaaa
     2101 tcagcacagc cagcagcggg gctgtgggaa cctgctccac gagcaggccg atactctcga
     2161 tggtagaagg atcggcccag tgcagatctt catagaccac cagcatcggc tggtgctcgg
     2221 cctcctccag cagccacgca gtcagcgtat cctgtgtttg ctgtttttcc tgctggggtg
     2281 tcagccccag cggcggatag cgctcttcgg gcagcggaag agagaacagc gcagcaaaca
     2341 gcggcacaac ctcctttaac ggcagactcg tagtttgcag tgtctgctcc attttggcaa
     2401 gtttctttgc cgggctgtcg tcccgctccc agcgccaaag ccgctgcagg tgggtggtca
     2461 ccggataaag cgcactgttg gtgtgataag gagaacagcg gtaggtgacg cgcgtatacc
     2521 cttcgctcgc tacatgttcg cgcaatacgt cggccaaggc ggacttgccg atacccggct
     2581 caccattgat tagcaccacc tggccgaatc cctctttact ctgttcccag cgccgcagca
     2641 acagccctat ttcctcgtcc cgtcccacca gggtggtaat gacaccggtt ccgacctcct
     2701 cctcgtcggt ttgtgccaca atggggttga gaactcttga cacggctatt agctctggca
     2761 cacctttgat gctattgctc ccaaggtcct ccagggcaaa tgcaccctcc acaaggcgct
     2821 tcgtcatcgc actgatcacc acagtgtcgg gctccgcgag ccgttcaagc cgggccgcca
     2881 cattgggggt ttcccccaaa gccaattgtt catgccgccc tccaccgccc atttctccca
     2941 ctacgaccgg tccggtgtga atgccaagcc gaacggcaag ttgaacgcca tactcggact
     3001 ccaagcgggt gttcagcttc cccatggcct cgacaatccc cagacctgcc tgcactgcgc
     3061 ggtgcgcatc gtcttcgtgt gcttggggat agccaaagta aaccagaagc ccgtcaccga
     3121 gatactgtgc tatgtggccg tcaaagccct gaattacatc tgctgccttt tcttggtagg
     3181 accgcacaac ctcgcgaagg tcttcggggt cgagtcgggc ggacagttcg gtcgatccaa
     3241 ccagatcgca aaacatcacg gtcagttgac gccgttcggc gcctgggagg cgggccttaa
     3301 acgcatgggc tgtcgtctgg ctgtcgttcg tggctgatgt ggacatggca ttcaaaggcg
     3361 ctgaatcatc tttggaaggg gcttctttgg gctcactaag attatgcccg catttgttgc
     3421 aaaacttaaa gcctgcagga ggttttgagc cgcagtttgg acaaactacc tctatttcca
     3481 gtgacgagcc gcatgctccg caaaacaggg agtcttcagg attctcaaat tggcattttg
     3541 gacattccat atttgaaacc tccgccgtgt gttgagataa aaacgatatg gcattccaat
     3601 tgtacgaaat ttataattca tgcctggata gcatgtcgtt ttcggtatct tcattatgcc
     3661 cattttaagg ttatctatga tgtggtagca tgcgtattct ctcagcagat gccataagca
     3721 tctgttacat cgaggagcat gccttagaac tgctcaagag cgaatttagc gcctatcgtt
     3781 tcaagtcgct aattttcccc cggctaattc aatccaaatt tcatgctctg ttttacccga
     3841 tttggaaagc ttgaaaccaa agattaattc aaccacccca agtataaaat atagaggctg
     3901 cgaaaaattg tgcaggccaa taaaccagac ttcaattgct atataaagta tcaaaaagac
     3961 accgaagaaa atcgctaagt ttccggtata cctggtacgt aaaaaagtaa ttatccctgc
     4021 cagcacatta ccaaccccat taaataccag aagaattaga ccgggaatga gataattgtt
     4081 gaaaggcaaa ttttttagca gcgccaacgg aatgttcatt ttggttccac tcgggtctga
     4141 taccaagccg aatccaccaa gaactgcggt aatacctaca aaggtttgaa gaactcctaa
     4201 tgcgtaagat ataaatttat ttttgatatc ctcattaata ttt
//
LOCUS       DBNGSX010000617         2400 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1731299, whole genome shotgun sequence.
ACCESSION   DBNGSX010000617 DBNGSX010000000
VERSION     DBNGSX010000617.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1731299"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="AC16CB_17780"
     CDS             complement(<1..263)
                     /locus_tag="AC16CB_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X swivel domain-containing protein"
                     /protein_id="HIR3344375.1"
                     /translation="MLIHGRTIFAGVAQGEALVTSMGISFYGGVDPESGIVVERGHEL
                     EGLSIAGKVLAFPTGKGSTVGSYTLYRLKKNGLAPAAILNSEC"
     gene            complement(343..1542)
                     /locus_tag="AC16CB_17785"
     CDS             complement(343..1542)
                     /locus_tag="AC16CB_17785"
                     /inference="COORDINATES: protein motif:HMM:NF016310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HIR3344376.1"
                     /translation="MKLTDSEQEMLDGVHGRATQKAMQILVALGTIYGAERMLPVSSV
                     QIAGVSYDNLGEAGLQFLTEMADGGGKARVLATLNPAGMDTENWQALGIPEDFARNQE
                     RVIEEFARMGVVTTCTCTPYLSGNLPQFGEHIAWAESSAVCYANSVLGARTNREGGPS
                     ALASALSGITPAYGMHLEQERQPELTIQVEAHVEGTHTFGALGMVMGQKLESTRRKPI
                     PYIVGIEHASLEELKTFCASLATYGGAALFHMAGITPESTRYSPPSTRIKITQADIAA
                     AQRSLNPSTEQDVDFVSLGCPHLSIQEIARLADLLKDKHVTKEFWITTARPTKEIADR
                     IGYTQIIEASGAKFAADTCCVVAPIRGRFQVMATDSAKACYYASGKNQFKTVFKPFDE
                     VVEEALR"
     gene            complement(1590..>2400)
                     /locus_tag="AC16CB_17790"
     CDS             complement(1590..>2400)
                     /locus_tag="AC16CB_17790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HIR3344377.1"
                     /translation="AFLGDLGEVEVAVCIGNPPNLMAAAATSVEVGVNELEIANALQP
                     LPVTRAKTVDVLVPAEAEFVLEGVVYRDRRHAEGPFVDLTETYDVVRQEPVFVVKTIT
                     HRKDAIWQALLPGALEHKLLMGMPREPTIYRKVNEVTRCLDVNVSPGGCSWLHAVVQI
                     DKQNENDGLVAIQAAFDGHRSCKHVFVVDKDIDIYDPQQVEWAMATRFQGDTGLVVRE
                     REPGSSLDPSAEAGTHLTTKIGFDLTKPFKTKGKSFEKAAFPKVDLRQFTG"
BASE COUNT          492 a          687 c          664 g          557 t
ORIGIN      
        1 tcacactcgc tattcaggat agcagcaggg gcaagtccgt ttttcttgag gcgatagagg
       61 gtgtacgatc ccaccgtaga gccttttccg gtagggaagg ccagcacttt gccggcgatg
      121 gatagccctt ccaactcgtg cccgcgctcc actacgatac cgctctctgg gtcgacgcca
      181 ccataaaagg agatgcccat ggaagtaacc agagcctctc cctgggctac ccctgcaaag
      241 attgttcttc catgtattaa catattcatg ccagggggtt tatccgcgaa taatgccaat
      301 tgcgcaaagg gaaataccag aaattggcgc aatggtggat aattatctca aagcctcctc
      361 aacgacttcg tcaaaaggct tgaagacagt tttgaattga ttcttcccgg aagcataata
      421 gcacgccttg gcgctatcgg ttgccataac ctggaaacgc ccgcggatgg gtgctaccac
      481 gcagcaagtg tcggcggcaa actttgctcc gctcgcctcg atgatttgcg tatagcctat
      541 gcggtcggcg atctccttag tcggtctggc agtggtgatc caaaattcct tggttacatg
      601 cttatccttg agcaagtcgg ccaatcgggc aatctcctgg attgagaggt gcgggcagcc
      661 caggctgacg aagtcgacgt cctgctcagt gctggggttc agtgaacgct gggcggctgc
      721 aatatcagcc tgggttatct taatccgggt cgatggtggt gaatagcggg tcgattctgg
      781 ggtgatcccg gccatatgga aaagcgcagc gccgccatag gtcgccaggc tggcgcagaa
      841 agttttcaac tcttccagcg atgcatgctc gatacctaca atgtatggga taggcttgcg
      901 acgggtcgat tcaagtttct ggcccatgac catccccaag gcgccaaagg tatgcgtacc
      961 ctccacatgt gcctccacct ggatggtcaa ttcaggctgc cgctcctgct ccagatgcat
     1021 gccgtaggcg ggtgttatac cgctgagggc ggacgccagg gcgcttggcc ctccctcccg
     1081 attggtgcgc gctcccagga cggagttggc ataacacacg gcactgctct cagcccaggc
     1141 gatatgctcc ccaaattggg gcaggttgcc cgagaggtat ggggtgcaag tacaggtggt
     1201 gaccactccc atacgagcga actcttcgat cacgcgctcc tggttgcggg cgaagtcctc
     1261 tgggatgcct agagcttgcc aattttcagt gtccatgcca gctgggttga gcgtggcgag
     1321 tacacgtgcc ttaccaccgc catcagccat ctcggtaagg aattgcagtc cagcctcacc
     1381 caggttgtca tagctcactc cggcgatctg taccgagctc accggcagca tgcgttcggc
     1441 gccatatatc gtgcctagtg caaccaggat ttgcatagcc ttctgggttg cccggccatg
     1501 cactccgtcc agcatttctt gttctgagtc tgtcaatttc atattgagta tagcccgctc
     1561 attcctgcca ctattaagcc atgcgcaggt tagccggtaa attgtctaag gtccactttc
     1621 gggaaagctg ccttctcaaa ggatttcccc ttggttttaa agggcttggt caggtcaaaa
     1681 ccgatttttg tggtgagatg cgtgcctgcc tctgcgctgg gatccaggct tgagccgggc
     1741 tctctctccc tgacgaccaa tccggtatct ccctgaaagc gcgtcgccat cgcccattca
     1801 acttgctggg gatcgtaaat gtcgatatcc ttatcaacca cgaagacatg cttgcaagag
     1861 cggtgaccgt caaaagcagc ctggatggct accagcccgt cattttcgtt ttgcttgtcg
     1921 atttgcacga ctgcgtgcag ccaggaacac ccgccagggc tgacgttcac atctaaacac
     1981 cgggtgacct cgttgacctt gcggtagatg gtgggctcgc gtggcatgcc catcagcagc
     2041 ttgtgctcca gcgcgccggg tagcaaggct tgccagatgg catctttacg atgtgtaatg
     2101 gtcttgacca cgaacactgg ctcctggcgc actacatcat aggtctcggt caggtcgacg
     2161 aatggacctt cggcgtgacg gcggtcgcgg tagaccacgc cctccaggac aaactcggct
     2221 tccgctggaa caagcacatc tacagttttg gcacgcgtca ctggcaaggg ctgcaatgcg
     2281 ttggcgatct ccagctcgtt gacgccgacc tctaccgagg tggctgctgc tgccatcaga
     2341 ttgggagggt tgccgatgca taccgccacc tcgacctcgc ccagatcgcc caggaaggca
//
LOCUS       DBNGSX010000618         2108 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_207289, whole genome shotgun sequence.
ACCESSION   DBNGSX010000618 DBNGSX010000000
VERSION     DBNGSX010000618.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_207289"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..638
                     /locus_tag="AC16CB_17795"
     CDS             183..638
                     /locus_tag="AC16CB_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016859385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HIR3344378.1"
                     /translation="MSNEEHMQKLLEGNEAWEAWRAKNPDTIPDLDGIDLSNADLSQS
                     NLKRADLRGSDLSGANLSEANLFKANLFKAKLIGADLSKANLSKATLLWANLSGADLS
                     GADLKEANLDGAVLDTANLSGANLSGAIYDSLTRWPDGFEVENSGAILC"
     gene            778..1203
                     /locus_tag="AC16CB_17800"
     CDS             778..1203
                     /locus_tag="AC16CB_17800"
                     /inference="COORDINATES: protein motif:HMM:NF013003.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HIR3344379.1"
                     /translation="MAEHEHLIQWQEGFEAWNKWRAENQDTRPNLLGADLSWASLLWA
                     DLNDTDMRRVNLNGANLSGARLIGADLRFANLTMTDLRGANMGGSNLSGANLTGANLT
                     GVNLHGADLSETRLTGARYNSATRWPIGFDVNASGAILG"
     gene            1417..>2108
                     /locus_tag="AC16CB_17805"
     CDS             1417..>2108
                     /locus_tag="AC16CB_17805"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3344380.1"
                     /translation="MLNSITPHVYWLPPDAATDRPILGAIVGEQRTLIVDAGNSPAHA
                     NLLLGELSKLHIAFPDYLVLTHWHWDHVFGSSAFKLPTFASKETRRIVAEMARLDWSD
                     AALDRRVEDGLEISFCRDMIKAEMPDRSNLTITPPDVAFDSPIQLDLGVLTCYIVPVG
                     GDHSSDASIIYVEEDKIMFLGDCLYTDIYSGPPRFTTRKLFPLLDRLLSFDTDYYLAA
                     HESNPMSRTDMV"
BASE COUNT          533 a          500 c          572 g          503 t
ORIGIN      
        1 agcccatcca ccaaagatag attatttgga ttacactcac cgtgggcagg gggatagagg
       61 tagaggcttt tatgctaaaa tcattcatct tttgttcgat gcagaactgc tacaaaatta
      121 tggtccgcta aaacgtacct cttgaaggaa tgcgctgaat ccatgcacaa ggaggtgtga
      181 acatgtcaaa tgaagaacat atgcagaagt tgctagaggg taatgaagct tgggaagctt
      241 ggcgagctaa aaatccagat actatacctg atctggatgg gattgacctg agtaatgccg
      301 acttgagcca gtccaacctg aaaagagctg acctgcgcgg gagcgacctc agtggagcca
      361 acctgagcga ggcaaatctt ttcaaggcca acctcttcaa ggccaagctc attggggcag
      421 acctgagcaa ggcgaacctg tccaaggcta ccctcctgtg ggctaacttg tcgggagcgg
      481 acttaagtgg ggctgatctc aaagaggcga acctggacgg ggctgttcta gacacggcaa
      541 acctgagtgg ggcaaatttg agtggggcga tctacgacag cctcacccgc tggccggatg
      601 gctttgaggt ggaaaattcc ggggctatac tctgctgaga ggacaatcat catgcttcaa
      661 cgggtgtgcc tgcgatgtca gcaggggtag gtgacttgat tttataatgg tagaccctgc
      721 ggtgtgcttt ctgattctta attgaattat cgacccaatg taccaggagt gttgcaattg
      781 gcagaacatg aacatctaat acagtggcaa gagggctttg aagcctggaa taagtggcga
      841 gctgaaaatc aagatacccg tccaaacctg cttggagcgg acctgagctg ggcaagcctg
      901 ttgtgggctg atctaaacga taccgatatg cgccgggtga acttgaatgg ggctaacttg
      961 agcggagcgc gcttgatcgg tgctgacctg aggtttgcca acctgaccat gaccgatctg
     1021 cgcggtgcca atatgggtgg gtctaacttg agcggcgcca acttgaccgg ggccaacctg
     1081 accggggtaa acctgcacgg cgctgacctg agcgagacca gactgaccgg ggcgcgctac
     1141 aacagcgcca cccgctggcc cattggcttt gatgtgaatg cttcaggggc tatactgggc
     1201 taatcatcgg tggttgatgg ctctctcttt ttcgatcagt ccttgcaggg gaacgagacg
     1261 agagggaaga ggggatggcg ttagacattg aatcagcaag ccgatcctgt aacaaattgg
     1321 gaaattgggc caaaatgagt atgtgagagc ctctaatctc cctggagcca tggtgatgct
     1381 caataaaagc ggatacaaat tgataaagct cattccatgt tgaattcaat cacgccccat
     1441 gtgtattggt taccacctga tgccgcaaca gatcgcccaa tcctgggtgc cattgtgggc
     1501 gagcaacgca cattgattgt tgatgcaggg aattctccag cgcacgccaa cctgttattg
     1561 ggtgaattgt cgaagctcca tattgcattt cccgattacc tagtgctcac tcactggcat
     1621 tgggatcatg tatttggatc atcagcattc aaattaccca ccttcgcttc aaaagaaacc
     1681 aggcgtattg tcgccgaaat ggcacgttta gactggagtg atgcggcgct ggatagacgc
     1741 gtcgaagacg ggctcgagat atcattttgc cgggatatga tcaaggctga gatgcccgac
     1801 cgtagcaatc tcaccattac gcctcccgat gtagcctttg attctcccat ccagcttgat
     1861 cttggagtcc tcacttgcta catagttccc gtgggaggcg atcactcatc agatgccagt
     1921 atcatctatg tggaagaaga caaaatcatg tttctgggtg attgtttata caccgatatt
     1981 tacagcggtc cgcccagatt cactacgcgt aaactatttc cgctcctcga ccgcctgcta
     2041 agttttgaca cggattacta tctcgcagcg catgagtcta accctatgtc cagaacggat
     2101 atggtcga
//
LOCUS       DBNGSX010000619         3604 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG05332
            SRR25158437_k127_1869721, whole genome shotgun sequence.
ACCESSION   DBNGSX010000619 DBNGSX010000000
VERSION     DBNGSX010000619.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414740
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:16:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,564
            CDSs (total)                      :: 3,525
            Genes (coding)                    :: 3,511
            CDSs (with protein)               :: 3,511
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 3 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1869721"
                     /isolate="MAG05332"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1318)
                     /locus_tag="AC16CB_17810"
     CDS             complement(146..1318)
                     /locus_tag="AC16CB_17810"
                     /inference="COORDINATES: protein motif:HMM:NF046269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HIR3344381.1"
                     /translation="MEHYTELLTKAISTLRQNDSGIFTKPGPRQYPHQWNWDSALIAL
                     GLSHFDLPRALTEIRALLTGQWRDGMLPHVVYHDKPSDYFPNPEFWQIKNSPDAPEVA
                     TSGITQPPVLASIIRQIHRRAPIRDFVDEVYPHLLRWHRWLHTARDADGSGLVYIIHP
                     WESGTDDSPRWLEVMSKITPHDLPVFQRGDTKHVAAEERPFREDYERFIYLIDIFRRN
                     NYEPSRLLAQSPFLIQDVLFNSVLFRANEDLRALALEVGESTAEVDDWLERMRANFDE
                     HFWDSQRGLYLDYDLRTGRRIPVNTAMTFLPLYAGIASQEKAARLIKTYWLNPDEYSP
                     GTRLHYAITSVSQKEPTWESRRYWRGPIWINLNWLLIIGLRSYGYDDLADRNTLLN"
     gene            complement(1629..2711)
                     /locus_tag="AC16CB_17815"
     CDS             complement(1629..2711)
                     /locus_tag="AC16CB_17815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3344382.1"
                     /translation="MAEIRIENVRKVYGKVIAVKDISLTIPDGQFIVLLGPSGCGKTT
                     LLRCIAGLEQVDQGRVYFDGKDITDLPVRKRAISMVFQSYAVFPHMKVYDNIAFGLRM
                     QRKSSSEVKQRVNAAAEMLHIEDLLDRFPAQLSGGQRQRVAVARAIAYDAKVLLMDEP
                     LSNLDALLRLEMRAELKRLLGGLGRTVVYVTHDQVEALSLGDRIAVMRYGEFLQVDSP
                     IRVYDYPAAYFVGSFIGNPPMNFLKAQVIEDAAAIQVEGSRLPLKPEISQALRSGGQQ
                     EILLGIRSENITPQKASTNGVLPARVLVVEPLGSHNLITVKVGETTVKVNAHPDMEFH
                     MEEKIFLALDVDKIRLIDPETEKAIL"
     gene            complement(2728..3561)
                     /locus_tag="AC16CB_17820"
     CDS             complement(2728..3561)
                     /locus_tag="AC16CB_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3344383.1"
                     /translation="MESKRKIPPSYVMSRVLVYLVAIVIALFIIIPIYLIAIMALSPR
                     DVIYEYPKPFIPNMVTFDTMQFFLNSYGILDSVKNSLVVAIVSLTLSLLVGAPAGYAL
                     ARYVFRGKDTYRVMILTTRAFPVVILSIPLITTYIRWGIDDTIQGVAFMHTAMALPTT
                     ILVSSSIFVALSKDLEEAAMTMGCSRFDAFRRIALPLALPGLAASAIFTFVLSWNEVF
                     AAAVLTVRNQTLPARLIVQLNQSPLPFRFAGGFFILIPAIIFMFFVRRYLLSLWGIKL
                     K"
BASE COUNT          977 a          905 c          806 g          916 t
ORIGIN      
        1 agggtctatc atcctaatca ccttcttcta gctacattat ttgcaatcat ttcaatcgcc
       61 aatgccgccg accatgagaa atccagcgag ccacaaccac tcccatcttg cggatcgtaa
      121 tactcgcgaa aaccccacct ttgcatcaat tcaagagagt gttacgatcc gcaagatcat
      181 cataaccata gctccttagc ccaataatga gaagccagtt caggttgatc caaattgggc
      241 cgcgccagta tcgtcgtgac tcccaagtcg gttctttctg gctgacactc gttatagcat
      301 agtgtagtct ggtcccaggt gagtattcat cagggtttaa ccagtacgtt tttattaacc
      361 tcgctgcctt ctcttggctc gctattcctg cataaagcgg gaggaaagtc atcgccgtat
      421 taactggaat gcgtcgtcct gtccgaagat cgtaatctag atataaaccg cgttgactat
      481 cccagaaatg ttcatcgaaa tttgcacgca tcctttccag ccaatcatcc acctcagctg
      541 tcgattcacc cacctctaaa gccagcgccc ttaagtcctc attcgcacgg aagagcacgg
      601 aattgaaaag cacatcttga attaaaaaag gtgattgtgc taaaagccgg ctaggttcat
      661 aattgtttcg acggaatata tcaatcaaat agataaagcg ttcataatct tcgcgaaaag
      721 ggcgctcttc cgcagcaaca tgtttggtat cacctcgttg gaatacaggc aggtcatgtg
      781 gagtgatttt gctcatgacc tccaaccagc gaggcgagtc atctgttccc gattcccagg
      841 ggtggataat ataaactagc ccgcttccat ccgcatcacg ggctgtgtga agccagcgat
      901 gccatcgcaa taggtgtggg tacacctcgt caacaaaatc ccgaatggga gcccgtctgt
      961 gaatttgcct aatgatgctg gcaagcaccg gaggttgggt gatgcctgat gtggcaactt
     1021 ctggggcgtc tggactgttt tttatctgcc agaattctgg attgggaaaa taatctgatg
     1081 gtttatcgtg atatacgaca tggggcagca taccatctcg ccattgtccg gttaacaaag
     1141 cccggatttc tgtcaatgct ctggggagat cgaaatgcga aagtcctaat gcaataaggg
     1201 ctgaatccca gttccactgg tgcgggtact gtcgcgggcc aggcttggta aaaatcccgc
     1261 tatcattttg tcgcagggta gatatggctt tggttagtaa ttctgtgtaa tgttccaatc
     1321 tcttttatat cttcctttgt gcttaatatt atcggagtta aattacttag cgaaacatag
     1381 aatcttagaa gtgattccaa aagtgatttt attccatgat gaatgatcta atagctctgg
     1441 acagacagca ttcgatcctc cacagtcgaa ccacctactc ctaagttagg ctcaggaata
     1501 gcattgacgc gttaaggtca accagtgatt gcctgttgga aagccgagtc tgccagttga
     1561 attgtcctta gggcatgatc cgaatcatgg acaccgagcg atattacctt aaaacaaagg
     1621 agcttttact acaggattgc cttctccgtc tccggatcaa tcaatcgaat tttatcaaca
     1681 tccagagcca agaagatctt ttcttccata tgaaattcca tatccggatg ggcgttcacc
     1741 tttacggtgg tttcacccac tttgacggtg atcaggttat gtgaccccaa gggttcaacg
     1801 accaacactc gtgcgggcag tacgccattc gtagacgctt tttgcggggt aatattttca
     1861 gaacggatac ctaacaagat ctcctgctgc ccaccgcttc gcaaagcctg gctgatttcc
     1921 ggcttgagcg gcaacctgct accctccacc tgtatggcag ccgcatcctc aatgacctgc
     1981 gctttcagga aattcatcgg tgggttgcca atgaagcttc ctacaaaata tgcagcgggg
     2041 taatcataaa cgcgtatcgg agaatccacc tgcaaaaatt cgccataccg catgaccgca
     2101 atgcggtcac ccaggctcag cgcttcaacc tgatcgtgag tcacatatac tacagttcga
     2161 cctaacccac ccagcagacg tttcaattca gcgcgcattt ccaatcgcag gagagcatct
     2221 agattgctta gtggctcgtc catcaataac acttttgcat cgtaggcaat cgcgcgcgcg
     2281 accgctactc gctgtcgctg ccctccactg agctgtgcag gaaagcgatc cagtaagtcc
     2341 tcgatgtgca acatttccgc agctgcgttc acgcgctgct tgacttcact acttgattta
     2401 cgctgcatgc gtagcccaaa ggcaatgtta tcgtagactt tcatatgcgg aaacactgca
     2461 tagctttgga agaccatcga aatcgcgcgc ttacgcactg gcaggtctgt gatatccttg
     2521 ccatcgaaat acaccctgcc ctgatccacc tgctccaagc cagcaataca acgcagcagt
     2581 gttgttttgc cacacccgct tggcccaagc aaaacgataa attgtccatc ggggatagtt
     2641 aaagaaatat ctttaactgc tataactttt ccatatacct tgcggacgtt ttcaatacgt
     2701 atctcggcca tatatcacct tctgcgatca cttaagctta atgccccaca ggctgagtaa
     2761 gtagcgccgt acaaaaaaca tgaagataat cgcggggatg aggataaaaa acccgccggc
     2821 gaatcgaaat ggcagtggcg actgattgag ttgaacaatc aacctagcgg gaagagtttg
     2881 gtttctcaca gtaagtactg ctgcagcaaa gacctcattc caggaaagaa caaaagtaaa
     2941 tatcgctgat gcagccagcc ctggtaatgc cagcggcaag gcaatcctac gaaaggcatc
     3001 gaatcgacta caccccatgg tcattgcggc ttcctccaaa tctttagaga gagccacaaa
     3061 gatactactg ctgaccagga tcgtggtagg aagcgccata gctgtatgca taaaagcaac
     3121 tccctggatt gtgtcatcga tgccccagcg gatatatgtc gtgataagag gaattgaaag
     3181 gatcaccact gggaaggcgc gcgttgtcag gatcattact cggtaagtat ctttcccgcg
     3241 aaaaacgtac cgcgccagag cgtagccagc tggcgctcca actaatagag aaagagtaag
     3301 tgagacaatc gccactacca agctattctt gaccgaatcc aggataccat acgagttaag
     3361 gaaaaactgc atggtatcga acgtcaccat attgggaatg aagggtttag gatattcata
     3421 gattacatcg cgtggactga gggccataat agcgatcaga tatataggaa tgatgataaa
     3481 caaggctatg acaattgcca ctagataaac caataccctg ctcattacat aagatggggg
     3541 aatttttcgt ttagattcca tcaatcttct cctttgatca gaccgatgtc ccctcttggg
     3601 agct
//
